LOCUS       DAZGBC010000001        89962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGBC010000001 DAZGBC010000000
VERSION     DAZGBC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.001"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHK1J_000001"
     CDS             complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHK1J_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO7909350.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2110..2976
                     /gene="yghU"
                     /locus_tag="ACHK1J_000002"
     CDS             2110..2976
                     /gene="yghU"
                     /locus_tag="ACHK1J_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO7909351.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="ACHK1J_000003"
     CDS             complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="ACHK1J_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO7909352.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3360..3701)
                     /gene="hypA"
                     /locus_tag="ACHK1J_000004"
     CDS             complement(3360..3701)
                     /gene="hypA"
                     /locus_tag="ACHK1J_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7909353.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIIYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3694..4182)
                     /gene="hybE"
                     /locus_tag="ACHK1J_000005"
     CDS             complement(3694..4182)
                     /gene="hybE"
                     /locus_tag="ACHK1J_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO7909354.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPLMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4175..4669)
                     /gene="hybD"
                     /locus_tag="ACHK1J_000006"
     CDS             complement(4175..4669)
                     /gene="hybD"
                     /locus_tag="ACHK1J_000006"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO7909355.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4669..6372)
                     /gene="hybC"
                     /locus_tag="ACHK1J_000007"
     CDS             complement(4669..6372)
                     /gene="hybC"
                     /locus_tag="ACHK1J_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO7909356.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6369..7547)
                     /gene="hybB"
                     /locus_tag="ACHK1J_000008"
     CDS             complement(6369..7547)
                     /gene="hybB"
                     /locus_tag="ACHK1J_000008"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO7909357.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7537..8523)
                     /gene="hybA"
                     /locus_tag="ACHK1J_000009"
     CDS             complement(7537..8523)
                     /gene="hybA"
                     /locus_tag="ACHK1J_000009"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO7909358.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8526..9644)
                     /gene="hybO"
                     /locus_tag="ACHK1J_000010"
     CDS             complement(8526..9644)
                     /gene="hybO"
                     /locus_tag="ACHK1J_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO7909359.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVIGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9833..10120)
                     /gene="yghW"
                     /locus_tag="ACHK1J_000011"
     CDS             complement(9833..10120)
                     /gene="yghW"
                     /locus_tag="ACHK1J_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO7909360.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10239..11126)
                     /gene="yghX"
                     /locus_tag="ACHK1J_000012"
     CDS             complement(10239..11126)
                     /gene="yghX"
                     /locus_tag="ACHK1J_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO7909361.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11283..12323
                     /gene="gpr"
                     /locus_tag="ACHK1J_000013"
     CDS             11283..12323
                     /gene="gpr"
                     /locus_tag="ACHK1J_000013"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO7909362.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12363..12857)
                     /gene="yqhA"
                     /locus_tag="ACHK1J_000014"
     CDS             complement(12363..12857)
                     /gene="yqhA"
                     /locus_tag="ACHK1J_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO7909363.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13048..13932
                     /gene="yghA"
                     /locus_tag="ACHK1J_000015"
     CDS             13048..13932
                     /gene="yghA"
                     /locus_tag="ACHK1J_000015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7909364.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIHLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVS
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14204..14629)
                     /gene="exbD"
                     /locus_tag="ACHK1J_000016"
     CDS             complement(14204..14629)
                     /gene="exbD"
                     /locus_tag="ACHK1J_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO7909365.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14636..15370)
                     /gene="exbB"
                     /locus_tag="ACHK1J_000017"
     CDS             complement(14636..15370)
                     /gene="exbB"
                     /locus_tag="ACHK1J_000017"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7909366.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15622..16809
                     /gene="metC"
                     /locus_tag="ACHK1J_000018"
     CDS             15622..16809
                     /gene="metC"
                     /locus_tag="ACHK1J_000018"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO7909367.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16949..17608
                     /gene="yghB"
                     /locus_tag="ACHK1J_000019"
     CDS             16949..17608
                     /gene="yghB"
                     /locus_tag="ACHK1J_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO7909368.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17648..18547)
                     /gene="yqhC"
                     /locus_tag="ACHK1J_000020"
     CDS             complement(17648..18547)
                     /gene="yqhC"
                     /locus_tag="ACHK1J_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO7909369.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18741..19904
                     /gene="yqhD"
                     /locus_tag="ACHK1J_000021"
     CDS             18741..19904
                     /gene="yqhD"
                     /locus_tag="ACHK1J_000021"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO7909370.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20009..20836
                     /gene="dkgA"
                     /locus_tag="ACHK1J_000022"
     CDS             20009..20836
                     /gene="dkgA"
                     /locus_tag="ACHK1J_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO7909371.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21036..21962
                     /gene="yqhG"
                     /locus_tag="ACHK1J_000023"
     CDS             21036..21962
                     /gene="yqhG"
                     /locus_tag="ACHK1J_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO7909372.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSVRIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITETD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRIDEIRVLEKTADSARIHIRFTLTNGNNEEQELVLQR
                     RDGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22011..22268
                     /gene="yqhH"
                     /locus_tag="ACHK1J_000024"
     CDS             22011..22268
                     /gene="yqhH"
                     /locus_tag="ACHK1J_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO7909373.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22311..24530)
                     /gene="ygiQ"
                     /locus_tag="ACHK1J_000025"
     CDS             complement(22311..24530)
                     /gene="ygiQ"
                     /locus_tag="ACHK1J_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO7909374.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGGRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24641..26053)
                     /gene="ftsP"
                     /locus_tag="ACHK1J_000026"
     CDS             complement(24641..26053)
                     /gene="ftsP"
                     /locus_tag="ACHK1J_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO7909375.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26128..26865)
                     /gene="plsC"
                     /locus_tag="ACHK1J_000027"
                     /gene_synonym="parF"
     CDS             complement(26128..26865)
                     /gene="plsC"
                     /locus_tag="ACHK1J_000027"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO7909376.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27099..29357)
                     /gene="parC"
                     /locus_tag="ACHK1J_000028"
     CDS             complement(27099..29357)
                     /gene="parC"
                     /locus_tag="ACHK1J_000028"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO7909377.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMLRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29495..31102)
                     /gene="ygiS"
                     /locus_tag="ACHK1J_000029"
     CDS             complement(29495..31102)
                     /gene="ygiS"
                     /locus_tag="ACHK1J_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7909378.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTILQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31211..31693)
                     /gene="ygiV"
                     /locus_tag="ACHK1J_000030"
     CDS             complement(31211..31693)
                     /gene="ygiV"
                     /locus_tag="ACHK1J_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO7909379.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(31746..32138)
                     /gene="ygiW"
                     /locus_tag="ACHK1J_000031"
     CDS             complement(31746..32138)
                     /gene="ygiW"
                     /locus_tag="ACHK1J_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO7909380.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32290..32949
                     /gene="qseB"
                     /locus_tag="ACHK1J_000032"
     CDS             32290..32949
                     /gene="qseB"
                     /locus_tag="ACHK1J_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO7909381.1"
                     /translation="MRVLLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALGERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32946..34295
                     /gene="qseC"
                     /locus_tag="ACHK1J_000033"
     CDS             32946..34295
                     /gene="qseC"
                     /locus_tag="ACHK1J_000033"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO7909382.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFVRTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(34341..34673)
                     /gene="ygiZ"
                     /locus_tag="ACHK1J_000034"
     CDS             complement(34341..34673)
                     /gene="ygiZ"
                     /locus_tag="ACHK1J_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO7909383.1"
                     /translation="MLKHEIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLDSGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIIIILRKLFVREMFEFWLYVCSFVICLVCGWWLF
                     WGRFIFCY"
     gene            35031..35612
                     /gene="mdaB"
                     /locus_tag="ACHK1J_000035"
     CDS             35031..35612
                     /gene="mdaB"
                     /locus_tag="ACHK1J_000035"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO7909384.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35643..35957
                     /gene="ygiN"
                     /locus_tag="ACHK1J_000036"
     CDS             35643..35957
                     /gene="ygiN"
                     /locus_tag="ACHK1J_000036"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO7909385.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36005..37897)
                     /gene="parE"
                     /locus_tag="ACHK1J_000037"
     CDS             complement(36005..37897)
                     /gene="parE"
                     /locus_tag="ACHK1J_000037"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO7909386.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(37926..38507)
                     /gene="yqiA"
                     /locus_tag="ACHK1J_000038"
     CDS             complement(37926..38507)
                     /gene="yqiA"
                     /locus_tag="ACHK1J_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO7909387.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38507..39334)
                     /gene="cpdA"
                     /locus_tag="ACHK1J_000039"
     CDS             complement(38507..39334)
                     /gene="cpdA"
                     /locus_tag="ACHK1J_000039"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO7909388.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39359..39781)
                     /gene="yqiB"
                     /locus_tag="ACHK1J_000040"
     CDS             complement(39359..39781)
                     /gene="yqiB"
                     /locus_tag="ACHK1J_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO7909389.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39782..40411)
                     /gene="nudF"
                     /locus_tag="ACHK1J_000041"
     CDS             complement(39782..40411)
                     /gene="nudF"
                     /locus_tag="ACHK1J_000041"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO7909390.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWV"
     gene            40616..42097
                     /gene="tolC"
                     /locus_tag="ACHK1J_000042"
     CDS             40616..42097
                     /gene="tolC"
                     /locus_tag="ACHK1J_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO7909391.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42245..42916
                     /gene="ygiB"
                     /locus_tag="ACHK1J_000043"
     CDS             42245..42916
                     /gene="ygiB"
                     /locus_tag="ACHK1J_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO7909392.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            42922..44082
                     /gene="ygiC"
                     /locus_tag="ACHK1J_000044"
     CDS             42922..44082
                     /gene="ygiC"
                     /locus_tag="ACHK1J_000044"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7909393.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(44120..44908)
                     /gene="ygiD"
                     /locus_tag="ACHK1J_000045"
     CDS             complement(44120..44908)
                     /gene="ygiD"
                     /locus_tag="ACHK1J_000045"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO7909394.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45051..45824
                     /gene="zupT"
                     /locus_tag="ACHK1J_000046"
     CDS             45051..45824
                     /gene="zupT"
                     /locus_tag="ACHK1J_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO7909395.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(45882..46052)
                     /gene="yqiD"
                     /locus_tag="ACHK1J_000047"
     CDS             complement(45882..46052)
                     /gene="yqiD"
                     /locus_tag="ACHK1J_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO7909396.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46314..46967)
                     /gene="ribB"
                     /locus_tag="ACHK1J_000048"
     CDS             complement(46314..46967)
                     /gene="ribB"
                     /locus_tag="ACHK1J_000048"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO7909397.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47071..47219)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47341..47640
                     /gene="ubiK"
                     /locus_tag="ACHK1J_000049"
     CDS             47341..47640
                     /gene="ubiK"
                     /locus_tag="ACHK1J_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO7909398.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(47675..47875)
                     /gene="glgS"
                     /locus_tag="ACHK1J_000050"
     CDS             complement(47675..47875)
                     /gene="glgS"
                     /locus_tag="ACHK1J_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO7909399.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            48144..48773
                     /gene="yqiJ"
                     /locus_tag="ACHK1J_000051"
     CDS             48144..48773
                     /gene="yqiJ"
                     /locus_tag="ACHK1J_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7909400.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            48800..50461
                     /gene="yqiK"
                     /locus_tag="ACHK1J_000052"
     CDS             48800..50461
                     /gene="yqiK"
                     /locus_tag="ACHK1J_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7909401.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            50700..50759
                     /locus_tag="ACHK1J_000053"
     CDS             50700..50759
                     /locus_tag="ACHK1J_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7909402.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            51076..51135
                     /gene="ibsE"
                     /locus_tag="ACHK1J_000054"
     CDS             51076..51135
                     /gene="ibsE"
                     /locus_tag="ACHK1J_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO7909403.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(51255..52688)
                     /gene="hldE"
                     /locus_tag="ACHK1J_000055"
     CDS             complement(51255..52688)
                     /gene="hldE"
                     /locus_tag="ACHK1J_000055"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO7909404.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQQARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(52736..55576)
                     /gene="glnE"
                     /locus_tag="ACHK1J_000056"
     CDS             complement(52736..55576)
                     /gene="glnE"
                     /locus_tag="ACHK1J_000056"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO7909405.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTVALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSENDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPTHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDVMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(55599..56900)
                     /gene="ygiF"
                     /locus_tag="ACHK1J_000057"
     CDS             complement(55599..56900)
                     /gene="ygiF"
                     /locus_tag="ACHK1J_000057"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO7909406.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAEEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            57142..57762
                     /gene="ygiM"
                     /locus_tag="ACHK1J_000058"
     CDS             57142..57762
                     /gene="ygiM"
                     /locus_tag="ACHK1J_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO7909407.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            57826..59064
                     /gene="cca"
                     /locus_tag="ACHK1J_000059"
     CDS             57826..59064
                     /gene="cca"
                     /locus_tag="ACHK1J_000059"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO7909408.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(59245..60066)
                     /gene="bacA"
                     /locus_tag="ACHK1J_000060"
     CDS             complement(59245..60066)
                     /gene="bacA"
                     /locus_tag="ACHK1J_000060"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7909409.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(60157..60525)
                     /gene="folB"
                     /locus_tag="ACHK1J_000061"
     CDS             complement(60157..60525)
                     /gene="folB"
                     /locus_tag="ACHK1J_000061"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO7909410.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            60630..61247
                     /gene="plsY"
                     /locus_tag="ACHK1J_000062"
     CDS             60630..61247
                     /gene="plsY"
                     /locus_tag="ACHK1J_000062"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO7909411.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(61260..62192)
                     /gene="ttdR"
                     /locus_tag="ACHK1J_000063"
                     /gene_synonym="dan"
     CDS             complement(61260..62192)
                     /gene="ttdR"
                     /locus_tag="ACHK1J_000063"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO7909412.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            62399..63310
                     /gene="ttdA"
                     /locus_tag="ACHK1J_000064"
     CDS             62399..63310
                     /gene="ttdA"
                     /locus_tag="ACHK1J_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO7909413.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            63307..63912
                     /gene="ttdB"
                     /locus_tag="ACHK1J_000065"
     CDS             63307..63912
                     /gene="ttdB"
                     /locus_tag="ACHK1J_000065"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO7909414.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            63961..65424
                     /gene="ttdT"
                     /locus_tag="ACHK1J_000066"
     CDS             63961..65424
                     /gene="ttdT"
                     /locus_tag="ACHK1J_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO7909415.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLMITGLLWMPVVLL"
     gene            complement(65467..66480)
                     /gene="tsaD"
                     /locus_tag="ACHK1J_000067"
     CDS             complement(65467..66480)
                     /gene="tsaD"
                     /locus_tag="ACHK1J_000067"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO7909416.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            66718..66933
                     /gene="rpsU"
                     /locus_tag="ACHK1J_000068"
     CDS             66718..66933
                     /gene="rpsU"
                     /locus_tag="ACHK1J_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO7909417.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            67044..68789
                     /gene="dnaG"
                     /locus_tag="ACHK1J_000069"
     CDS             67044..68789
                     /gene="dnaG"
                     /locus_tag="ACHK1J_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO7909418.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            68984..70825
                     /gene="rpoD"
                     /locus_tag="ACHK1J_000070"
     CDS             68984..70825
                     /gene="rpoD"
                     /locus_tag="ACHK1J_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO7909419.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(70904..71410)
                     /gene="mug"
                     /locus_tag="ACHK1J_000071"
     CDS             complement(70904..71410)
                     /gene="mug"
                     /locus_tag="ACHK1J_000071"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO7909420.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            71535..71610
                     /locus_tag="ACHK1J_000072"
     tRNA            71535..71610
                     /locus_tag="ACHK1J_000072"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71568..71570,aa:Ile,seq:cat)
     gene            complement(71664..72428)
                     /gene="nfeF"
                     /locus_tag="ACHK1J_000073"
                     /gene_synonym="yqjH"
     CDS             complement(71664..72428)
                     /gene="nfeF"
                     /locus_tag="ACHK1J_000073"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO7909421.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            72716..73339
                     /gene="nfeR"
                     /locus_tag="ACHK1J_000074"
                     /gene_synonym="yqjI"
     CDS             72716..73339
                     /gene="nfeR"
                     /locus_tag="ACHK1J_000074"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO7909422.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(73446..74966)
                     /gene="aer"
                     /locus_tag="ACHK1J_000075"
     CDS             complement(73446..74966)
                     /gene="aer"
                     /locus_tag="ACHK1J_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO7909423.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSEMVKHRASRLEDAVTVLH"
     gene            75384..76763
                     /gene="ygjG"
                     /locus_tag="ACHK1J_000076"
     CDS             75384..76763
                     /gene="ygjG"
                     /locus_tag="ACHK1J_000076"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO7909424.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(76805..77137)
                     /gene="ygjH"
                     /locus_tag="ACHK1J_000077"
     CDS             complement(76805..77137)
                     /gene="ygjH"
                     /locus_tag="ACHK1J_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO7909425.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            77356..78339
                     /gene="ebgR"
                     /locus_tag="ACHK1J_000078"
     CDS             77356..78339
                     /gene="ebgR"
                     /locus_tag="ACHK1J_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO7909426.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            78523..81615
                     /gene="ebgA"
                     /locus_tag="ACHK1J_000079"
     CDS             78523..81615
                     /gene="ebgA"
                     /locus_tag="ACHK1J_000079"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO7909427.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHVEVRAEGETLTTRQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGKRYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            81612..82061
                     /gene="ebgC"
                     /locus_tag="ACHK1J_000080"
     CDS             81612..82061
                     /gene="ebgC"
                     /locus_tag="ACHK1J_000080"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO7909428.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            82124..83557
                     /gene="ygjI"
                     /locus_tag="ACHK1J_000081"
     CDS             82124..83557
                     /gene="ygjI"
                     /locus_tag="ACHK1J_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7909429.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            83691..84761
                     /gene="ygjJ"
                     /locus_tag="ACHK1J_000082"
     CDS             83691..84761
                     /gene="ygjJ"
                     /locus_tag="ACHK1J_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO7909430.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            84778..87129
                     /gene="ygjK"
                     /locus_tag="ACHK1J_000083"
     CDS             84778..87129
                     /gene="ygjK"
                     /locus_tag="ACHK1J_000083"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO7909431.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKSDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            87949..>89962
                     /gene="fadH"
                     /locus_tag="ACHK1J_000084"
     CDS             87949..>89962
                     /gene="fadH"
                     /locus_tag="ACHK1J_000084"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO7909432.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ANQLQEFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT        22011 a        24200 c        22794 g        20957 t
ORIGIN      
        1 tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact ttttcaccac
       61 aattaacggt tcaatatcac tctctttttt aataaccagt gattcatcac cgcgcaaaca
      121 cgtcccaccg tagttgccgc caacggtgaa ggtacatacc tgaatgtatt taccgtccac
      181 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccactggt
      241 tttgtccagc acctcttcat gatggctgac gaggtcgatg ttactgccac agcgaccggc
      301 gatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga aatcggtatc
      361 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
      421 ggggatcacc gtccacagcg gctcaaagac cagcacttcc gggcgcagca atacgtcgat
      481 aagacgcact tcattttgcg gatgaccggt acggatcggc accgcagcaa actcacggtc
      541 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
      601 aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca gttcatccag
      661 cccgcgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca taaactgcgc
      721 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
      781 gtgtttccag gcaccagcca gttcgttaat cagcccttcc gccggattga agccgttgcc
      841 tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat gacatgaggc
      901 ggagtcggcg ttgtactcgt aaaccttcag accacgctca tccatgcaga aatccatacg
      961 accggtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     1021 tttcggaatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg
     1081 aagatacatc aggtgcagct cgttggtggc tttaatcagc tcctgctcgg cgctctcggt
     1141 aatggtgtag taatgatacg gatcctgatt gatcacctga ccgttggcct gcacataggc
     1201 gttttgcagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     1261 acgcgctccg ctgattttca gcagctcgcc tgcaatttcc ggctgcggta agctgtattc
     1321 agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag tgtctttaag
     1381 ggtatagcag ccgttttcga ccaccatctc cagttcgcgc gtccactgtt gcccttgcgg
     1441 caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gtaattgggt
     1501 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     1561 acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
     1621 attaaccact tcacgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
     1681 aaagaccaca ccgtagttca gaaagagaaa acggcgagca aattcaacgc attgccattt
     1741 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     1801 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     1861 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     1921 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     1981 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     2041 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     2101 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
     2161 gctggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg
     2221 ctgccggtgg gcaaacatcc attacaactt tattcgctgg gaacgccgaa cggtcagaaa
     2281 gtaacgatta tgcttgagga gctgctcgcg ctgggcgtta ctggcgcaga gtacgatgcc
     2341 tggctgatac gcataggcga tggtgatcaa ttctccagcg gctttgtcga agtgaaccca
     2401 aattcgaaga tcccggcgct gcgcgatcat acccataatc cgccgatccg cgtgtttgag
     2461 tcaggatcga ttctgcttta tctggcggag aaatttggct acttcctgcc gcaggatctg
     2521 gcaaaacgga ctgaaacgat gaactggctg ttctggttac agggtgctgc gccgttcctc
     2581 ggcggtggtt ttggtcactt ttaccattac gcgccggtga aaattgagta cgccatcaat
     2641 cgctttacca tggaagccaa gcgccttctc gacgtgctgg ataagcaact ggcgcagcat
     2701 aagtttgttg cgggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
     2761 aatgtggtgt taggcggcgt atatgatgcc gcagagtttc ttgatgcggg cagttataag
     2821 catgtgcaac gctgggcaaa agaagtgggc gaacgtccgg cggtgaaacg tgggcgtatt
     2881 gttaaccgca ccaacgggcc gctgaacgag cagttgcatg agcgccatga cgccagtgat
     2941 tttgagacga atacggaaga taagcgtcag gggtaagggt tagcgttcgt cacagcaagc
     3001 catccaggcc ggataaggca ttcacgccgc atccggcaat cgagcacaat gcctgatgcg
     3061 acgctgccgc gtcttatcag acctacgaaa gctaatcatc acgcgctggt aatgtcgtaa
     3121 tccatttggc gcagtgcgtc taatgtggct ttggcttcat cttcgtcgat gatgctcatg
     3181 gcaaatccga cgtgtaccag cacccactgg cccagtagat cggcagggtt accttcacaa
     3241 atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc aagctggtga
     3301 atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattgt taactccggt
     3361 tattcaactt caatactttt gacgatcagc gaatcgccgg tatcgacacg caaccgctcg
     3421 ccatgacaga gcggacactg cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc
     3481 cagcaccagg cctgggcggg tttatagatg atatgtaaat cgcacccttg cgccaccgtt
     3541 ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca ggagagcgcg
     3601 ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc ctgccgctgg
     3661 ataatttcaa cggcgctctg gcaaagcgac aactcatgca ttttcgccac tcctgcgacc
     3721 aaacagcagg gcgcgacgcc ctgcgtgtgg tacatccgga ttggtgaccg gcagcgaaag
     3781 gatcattcgt gcgcagtcat ccgtcaggcg ttgaccctct tcaatcgaca tactgtgcga
     3841 aagcggtgac atcagcgaac aggagaggta ttgcgaaaca ccgtccagtt cgccaacggt
     3901 aaaggtcatg gtgccatacg gcagttgcag accgattttt tcactcactt tgcgcagcgg
     3961 ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg tgatcacaca
     4021 ccccgtccac tgaccttcga acagcgtaaa atcagaaaca tacaccggca ttaaaggatg
     4081 cagaaaagag agatcgtgca tcgaacgccg ggcaatttct tcaaacgctg cctgtacttg
     4141 cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgaatcgcc tcccgtggga
     4201 tggcttccac gccagattca cgcagcgcag ccagaacctg ctcaagcgca ggttcaatca
     4261 tcgcttcgac cgtcggcgtc aagccgatgt gcggctccag tgattccgga attacgccga
     4321 ccagggtcag ctttttcgga aactcgccgg tgaagcgcag ggccgacaag acgtcggcca
     4381 ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct tcatcccgca
     4441 ggatcatcat cgttcccggc gcgctcttct tcgacacaat ggcatccgca ataatcagat
     4501 gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc cgtgccgcca tcgaggatct
     4561 caacataatc cggcagaatg tatcgttgct ctaacgcttc gacaatccgc acaccgatgg
     4621 cttcatcggt cagcaaaata ttgccgaccc ctaagactaa aatacgcatt acagaacctt
     4681 cactgaaacc acttcgttgc cgtcagcatc cactacgtgt accgcacagg ccatgcacgg
     4741 gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcgg caaccggtgt
     4801 acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg gaccagagtt
     4861 ccaggttgat ggaacaaccg cctggtagtt gctgatgata ccgtctttaa taaccatcca
     4921 gtgagagagc ataccgcgcg gcgcttcgag gaagccaaca cctttgaact cacccgttgc
     4981 cggaatgttc ggcttcacaa aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc
     5041 actgtattgg ttttgcagga tatcctgcaa ttcacagcag tgaacggtac gaccaataat
     5101 acggcccagc gtggagtgca gctgtgccac ttccagcgtg ttgccagtca gtttctgata
     5161 aatcgcaacg atttcattca gtttgttctg ggtagattcg cgacctgccg ccagtttcac
     5221 cagcatatta gccagcggac ccacttctac cgttttgccg tagaaagtcg gtgatttcac
     5281 ccaggaatat ttcccgtcgt cagaccaacc atcataagcc ggaatggtgg taccttccca
     5341 cggtgcctgc ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct gaatgccttt
     5401 gatcaggtat tcatcggaat gagaagtgat cggacgatac gaggacagat ccgcattctc
     5461 aatgtagccg cccgggaaca ggaagctgcc gtttttactg tcggtcggga attccggcac
     5521 gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga acgcagcaat
     5581 aactgcggta tcaaccttat aaacctgttc aacaaagtcg ctcagcttgt cgatgaaaga
     5641 cttgatgtac atcaggcgct caaggttcag cacgcccaga ccgtcgaggt tgattgggtt
     5701 agcgacccca cctaccgcca ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac
     5761 gacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta ccgcgatcag
     5821 gttcacttcc ggcggcagtt tcatcgccgg gtgaccccag tagccattgg cgaaaatacc
     5881 caactgaccg ctggcaacca gatctttgat cttgttctga actttagtga actcttccgg
     5941 actgttcaga tgccaggtcg aaacgccttt cagcatttcg gaggctttgg ttgggtcagc
     6001 ttgcagtgca gaagtgatgt ccacccagtc cagcgccgaa agctgataga aatgaacgat
     6061 atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg cgttaaccgg
     6121 aacgtcgata ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt gagtggtagt
     6181 acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac
     6241 gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa cgacgccatt
     6301 ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg gttaccggat caatagtaat
     6361 tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt taaaggagga
     6421 agtatcggca gtagacgaat gagtacgatg taagcgcaaa tctcaatagc cacaaaacca
     6481 atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat
     6541 gccaccagcg aataggtcag acgccaggtt gcacaaccta acagcgcgct cagtgccgac
     6601 aggaacagca tgcgggaatc attacgcagc ttcgccacac gcagaaccac cagcgggaag
     6661 agcatcagca ggacttcaat ccagaacatc acggagtaga agtcaccggc aaacgctaac
     6721 gacagcttgt cgcgatagat aagctcgcca aagcgcagca cgatgaaaat cgccagcaac
     6781 acactgatgg tgttggtcag cttaacgaac agactctttt catccggacc gttgccacgc
     6841 agacccgcct gcaccagcga accttcaaag atgacaatcg agaagcccat gatgaacgcc
     6901 gtcagcagcg agaacagcgg caacatttca tagctctgcc acagcggatg caccttgtag
     6961 cccgccgaga tcatcagcga ccccattgaa gactggtgca tggtcggcag cagcgcaccg
     7021 agcgcgatga tgaagaacat caccttgttc agacgctgta gcgacacctt ccagcccaga
     7081 cgttcaaaca gtgccggagc aaactccaga gccatcacgc cgatgtagat ggtcatacag
     7141 accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta gaagtacggc
     7201 aggttccagt agcgacccac gtcgatagtg atcgacaagc cacccagtga gtaaccaaac
     7261 agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt aaagacgtat
     7321 accgcccacg ccagcgccca gccgccacag gcaaagccgg tgccaatcag caggtcaaac
     7381 gcgatccaca cgccccacgg gaagccgccg ttcaggtcag agacagagcc cagaccgaac
     7441 accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa aatcatgacc
     7501 ggtttactga tgattttgcc gcccagcggt tttggatcat gactcatgat cgtctcctcc
     7561 gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg ccagcacagc
     7621 cagtggtagc atcatgcctt tatacagggt gtgttgaata ttttcggaac gcgcaccggt
     7681 agaaagatcg tccagtttcg gcaggtcgag attttcataa ggcacacccg tcagtaccag
     7741 aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt tcggcaccgt
     7801 atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt cgctgccagg
     7861 cttcagcgcc agacgttttt tcgcctccgc catcagctct tcacgcgtac caaaaatcac
     7921 cgcgcccgcc gggcacactt ctacgcagcc aggcagaccg cctttatcga gacgttccac
     7981 acctttctgg ttgcacagct cgcacttatg cagcgcacca aacgggttgt tgtagtcgta
     8041 cttcggcacg ttgtacggac aggcgaccat gcagtaacgg cagccggtgc acacgtcttt
     8101 gtcgtaatga acaatgccgg ttttcggatc ttttttcagt gcagagaccg ggcacacaga
     8161 gacacagttc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt agccgttctc
     8221 ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt tattggtata
     8281 cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac gttcagggaa
     8341 gttgatatcc tgacacttgg tgacgcaagc ctggcagcct acgcacaagg tcgagtcgta
     8401 caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat gactgacaga
     8461 cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat tacgtctgtt
     8521 cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc cagttcacgc
     8581 accgccatca cactgacacc ggcaaccagc ccaatcacac cgccgagcaa accaatagcg
     8641 cctgcagaga cgttgccgcc ctctttagcg ttaacatccg gtttctgtga acgcggagtc
     8701 tgattttcga cgttggcaag ctgatggatg cctttatgga agccgatacc ttcttcgtta
     8761 cagccatagc acgggtgacc aatcgccacc ggccacacac cgccaacatc gcagaattgc
     8821 agcgttgagc agttgccgta agtttctggc cctttacagc cgaggtggta caggcaccag
     8881 ccttcgcggt ggccttcatc accgaactct ttggcaaaac gaccagcatc gaagtgcggg
     8941 cgacgttcgc agtgttcgtg aatcagacgg ccataggcga aggtcggacg gtttttgtca
     9001 tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgcgag gaagttgtgc
     9061 gggttcggcg ggcagcccgg aatgttgata acggttttgc ctggcagaac ttcttgcagg
     9121 ctgactgcgc cagttgggtt aactccagct gcggcaacac cgccccacgc agagcaggaa
     9181 ccgatagcga taatggctgc tgcgccttcc gccgctttgc ggatgtgatc cacaatcggc
     9241 tcaccggcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc atccaccact
     9301 aacacatact gccctttgta cttctcgaga gcgttatgtt tgttctcttc gacctgatga
     9361 ccgaaggcgg cggaaagcac ttcgtgatac tccagagaga tagtctccag tacgaggttt
     9421 tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca ctcctgcgcg
     9481 ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcggccat ctctgcagcg
     9541 gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat gaaatcacga
     9601 cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag ttattccgtt
     9661 gcgaagacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca
     9721 tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc gctaaaacga
     9781 agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt gatcaatttt
     9841 cgaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc tcacgaaaga
     9901 aatccattac catctcttta tccagtccat agcggcgcgt cagaataccc gcttcccagg
     9961 cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct aatacaaaac
    10021 cgcgtttata atcggcacag aattttgtca cattaaccgc actgtcggca tgcgttgctt
    10081 ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacctc attcgctata
    10141 tatcgtcata tatagcgata ttttaggcaa agaacgactt ttgcgcacgc tattttttca
    10201 tgcttgttta agacgtaatc ccgtaagaga taaacctatc aggagagata tttatcgaac
    10261 catttcagtg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa
    10321 tcattatgga atccgtgatt aacccccgga tagatatacg cctcataaac cttattattg
    10381 gctttcaacg ccgcctcgta agcaggccag ccctcgttga ttcgggtgtc cagttccgcg
    10441 aagtggagta gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc ctgacgacca
    10501 taaaacggca ccgcgcaggc cagttccgga tacgcgacag ccgccgcgtt cgatacgcca
    10561 ccgccatagc aaaatccggt aatacccact ttgcctgtcg cttgcggata gcgttgcata
    10621 aactcaattg cggcaaagaa atcattcatc agtttggttg gatcaacctg ctgttgcagc
    10681 tcacgacctt tatcatcatt tcccggataa cctccaacgg aacttaagcc gtcaggtgcc
    10741 agggcgatat accccgcctt cgccactcgc cgtgccacat cttcgatata cggattcagt
    10801 ccacgattct catgcaccac caccacggct ggcgttttgc cgctcatttt tgcgggtttc
    10861 accagataac cccgtacctc gccgtgacca tttggcgaag gatacgtgat gtactcagca
    10921 acaatctccg ggtcggtaaa ctctacctga gtcgccagcg cataatttgg cttgagcaaa
    10981 tcaaacaacg ctaatgccgt catcccgccc accgcatact tcgccgcaag gttgaggaac
    11041 tcacgtttcg agattttccc gtgagcgtaa tagtcgtagt aatccaacaa ctcttgtggg
    11101 aaatctttgg cggttaaacg cggcatcgtt gcactcctca gctggtgttt ttttaagcaa
    11161 agcataagca cgtgtttttg cccagttttt cgtcactctg tgagccagac tacgggatac
    11221 gcgctggcga atcgctaaac tagaaacatt gtttcgaaat tgaacggtgg aaaggagagg
    11281 tcatggtctg gttagcgaat cccgaacgtt acgggcagat gcagtaccgc tattgcggaa
    11341 aaagcggttt acgcctgccc gcgttatcgc tcggtttatg gcacaatttc ggtcacgtta
    11401 acgcgctgga atcacagcgt gcgatcctgc gtaaagcgtt tgatttgggc attacgcact
    11461 ttgatttagc caacaattac gggccgcctc caggaagcgc agaagagaac tttggtcgcc
    11521 tgctgcggga ggattttgcc gcttatcgcg atgaactgat tatctctacc aaggctggct
    11581 acgatatgtg gcccggccct tacggctctg gcggttcacg taaatacctg ctcgccagcc
    11641 tcgaccaaag cctgaagcgt atggggcttg agtatgtcga tatcttttac tctcatcgcg
    11701 tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct ggctcatgcg gtacaaagcg
    11761 gtaaggcgct gtatgtcggg atctcctctt actcgccaga gcggacgcaa aaaatggtcg
    11821 agttgctgca cgagtggaaa attccgctgt taattcatca accttcgtac aatttactaa
    11881 accgctgggt ggataaaagc ggcctgctgg ataccctgca aaataacggc gtgggctgca
    11941 ttgcctttac tcctctggct cagggattgc tgaccggaaa atatctcaac ggcattcctg
    12001 aagattcacg gatgcatcgt gaagggaata aagttcgtgg tctgacgccg aaaatgctca
    12061 ccgaagccaa cctcaacagc ctgcgcttat tgaatgaaat ggcacagcag cgtggacaat
    12121 caatggcgca aatggcgtta agctggttgc tgaaagatga gcgagtgacg tcggtattgg
    12181 ttggtgccag ccgcgcggag caacttgagg agaacgtgca ggcgctgaat aatctgacat
    12241 ttagcaccga ggagctggcg cagatcgatc agcatatcgc cgatggcgag ctgaatctgt
    12301 ggcaggcgtc ttccgataaa tgacctgtta ataacgggcg cgagaaccgc gcccgaatca
    12361 gatcagtgat tatgacgagt cagtcggtca agatagccca ttacaaatgc agagagcaca
    12421 aacgtcagat ggataatgac gtaccacatc agtttgttat cagggacatt tttcgcatcc
    12481 ataaagacgc gcagtaagtg aatggaagaa attgccacaa tcgacgctgc tactttgttt
    12541 ttcagcgacg ttgcgtccat tttccccagc cagttcagct tctctttgtt ctcggagata
    12601 tcgagttgtg agacgaaatt ctcataaccg gaaaacatca ccatcaccag caaaccgcca
    12661 accagcgtca tatccaccag cgacagcaac acgaggatca aatctgattc cgccatcgag
    12721 aagatattcg gcagtacgtg aataatctcc tggaagaact tcagcgccag ggcaactaac
    12781 gcaagcgaaa ggccaaagta cacgggggca agcagccagc gagaagcata cattgcattt
    12841 tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa cgggcaacag tatatcgcaa
    12901 ttgagtaaac agcgcgcaac agcaaatcaa catatccgtt aaacctttgt cgcataattc
    12961 tgagggttag ggatcttcct ggttccctta tcaacgcatc gagagcgtct acccttaaag
    13021 gacgttgaac ctgaagggag aaaaacgatg tctcatttaa aagacccgac cacgcagtat
    13081 tacactggtg aatatcccaa acagaaacaa ccgacgccag gcatccaggc gaagatgaca
    13141 ccggtaccgg attgcggcga gaaaacctat gttggtagcg gtcgcctgaa agatcgtaaa
    13201 gccctggtga cagggggcga ttccggaatc ggtcgcgctg ccgccatcgc ttacgcgcgt
    13261 gaaggggctg acgtggcgat cagttatctt cccgtggaag aagaagacgc tcaggatgtg
    13321 aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc tgccaggcga tttaagcgat
    13381 gagaaatttg cccgttcgct ggttcacgaa gcgcataagg ccttaggtgg gctggatatt
    13441 atggcgctgg tcgccgggaa acaggttgcc attcctgata ttgcagacct caccagcgaa
    13501 cagtttcaaa agacctttgc cattaacgtt ttcgcactgt tctggctaac ccaggaagcg
    13561 atccacctgc taccgaaagg tgcaagtatc atcaccactt cgtcaatcca ggcataccag
    13621 ccaagtccgc atttactgga ctatgcggct acgaaggcgg cgattctgaa ctacagccgt
    13681 ggcctggcaa aacaggtctc ggagaaaggt attcgggtga atattgtcgc gccaggcccg
    13741 atctggacag cactgcaaat ttccggcgga caaacgcagg ataagatccc gcagtttggt
    13801 cagaaaacgc cgatgaagcg tgcggggcaa ccggcggaac tggcccctgt atatgtttat
    13861 ctggcaagtc aggagtcgag ctacgtcacc gcagaagtgc acggcgtgtg cggcggcgag
    13921 catttaggtt aaaaaaatgc ccggttgtga aaagcaaccg ggcatcattg tgaattcact
    13981 cttacctgat gtggcgaaaa cgccttatcc gcctacaaac gcatgaagat tcaacatgtt
    14041 gcaatagatt ttgtaggcct gataagcgta gcgcatcagg cattgagcac ctaatgccgg
    14101 atgcggcgtg aacgccttat ccggcctaca aacgcacgaa gattcaacat gttgcaatag
    14161 attttgtagg cctgatgagc gtagcgcatc aggcaattct actttacttc gctttggcgg
    14221 tttcttcacc gaccagacct atcttcaggt aacccgcctg atgcagcgta tccattacct
    14281 tcatcaacgt ctcgtaatcg acggttttgt ccgctcggaa gaagatggtg gtgtctttct
    14341 taccttcggt taacgcattc aacgccgtaa tcattgtttc atcggtgacc ggatcgttac
    14401 cgataaacat cgagttgtct gccttcaccg acagataaac cggtttttcc ggacgcggct
    14461 gcggcgtgct ggtagaagca ggcaagttca ccttcacatc taccgtcgct aacggtgccg
    14521 ccaccataaa gataatcagc agaaccaaca tcacgtcgat aaacggcgtc acgttgatat
    14581 catgcatttc gccgttatcg tcgaggtttt cgttaagatg cattgccatc ggatattatc
    14641 ctgcacgtaa tttttgtgcg acacgaaccg gatgcgcagc ggcgctggct tccagatcca
    14701 ggtcacggct ttgcagcaac aatacctgcg ctgcgacatc acccagcatc gctttaaagc
    14761 cgccaatctg acgcgcgaat acgttataga taacgaccgc cggaatcgct gccaccagac
    14821 cgattgccgt tgctaacaga gcttctgcga tacccggcgc aacgactgcc aggttagtag
    14881 tctgcgtttg cgcgatacca ataaagctgt tcatgatgcc ccagaccgta ccaaacagcc
    14941 caacgaacgg cgaaatcgcg ccgatggttg ccagatagcc gttaccgcgg cccatttgac
    15001 gacctactgc ggcgacccga cgctccagac gaaagctggt acgttcttta ataccttcgt
    15061 tatcgtcgct gccttctgac agttccagct cattctgcgc ttcattgagc aaatgcaggc
    15121 ttaagctttt gctaccaaaa tcagcggcga tatcgttggc ctggtttaag gaacgcgctt
    15181 cagccagcag ttgctgctcg cgcttaaggc gacgcttctg attgaagaac tctacgctct
    15241 tactgaagaa gattgcccag gtgactacgg aggccaaaat aagcccaatc atcacgcact
    15301 taacgacaat atcggcgtgc tgatacatac cccagacgga aaggtccgtc tgcattaaat
    15361 tattacccac tgtgtatctc caggacgcaa gtcacaaaat ctgcgcataa taatatcaaa
    15421 acgacgtcga attgatagtc gttctcatta ctatttgcat actgccgtac ctttgctttc
    15481 ttttccttgc gtttacgcag taaaaaagtc accagcacgc catttgcgaa aattttctgc
    15541 tttatgccaa ttcttcagga tgcgcccgcg aatattcatg ctagtttaga catccagacg
    15601 tataaaaaca ggaatcccga catggcggac aaaaagcttg atactcaact ggtgaatgca
    15661 ggacgcagca aaaaatacac tctcggcgcg gtaaatagcg tgattcagcg cgcttcttcg
    15721 ctggtatttg agagtatgga agccaaaaag cacgcgacgc gcaatcgcgc caatggcgag
    15781 ttgttctatg gacggcgcgg aacgttaacc catttttcct tacaacaagc gatgtgtgaa
    15841 ctggaaggtg gcgcaggctg cgcgctattt ccctgcgggg cggcagcggt tgctaattcg
    15901 attcttgctt ttgtcgaaca gggcgatcat gtgttgatga ccaacaccgc ctatgaaccg
    15961 agtcaggatt tctgtagcaa aatcctcagc aaactgggcg taacgacatc gtggtttgat
    16021 ccgctgattg gtgccgatat cgttaagcat ctgcagccaa acactaaaat cgtgtttctg
    16081 gaatcgccgg gttccatcac tatggaagtc cacgacgttc cggcgattgt tgccgccgtg
    16141 cgcagtgtgg tgccggatgc catcattatg atcgacaaca cctgggcagc cggtgtgctg
    16201 tttaaggcgc tggattttgg catcgatgtt tctattcaag ccgccaccaa atatctggtt
    16261 gggcattcag atgcgatgat tggcactgcc gtgtgcaatg cccgttgctg ggagcagcta
    16321 cgggaaaatg cctatctgat gggccaaatg gtcgatgctg ataccgccta tataaccagc
    16381 cgtggcctgc gcacattagg tgtgcgtttg cgtcaacatc atgaaagcag tctgaaagtg
    16441 gccgaatggc tggcagaaca tccgcaagtt gcgcgagtta accaccctgc cctgccaggc
    16501 agtaaaggac acgaattctg gaaacgagac tttacaggca gcagcgggct attttccttt
    16561 gtgcttaaga aaaaactcaa tgatgaagag ctggcgaact atctggataa cttcagttta
    16621 ttcagcatgg cctactcgtg gggcgggtat gaatcgttga tcctggcaaa tcaaccagaa
    16681 catatcgccg ccattcgccc acaaggcgag atcgatttta gcgggacctt gattcgcctg
    16741 catattggtc tggaagatgt cgacgatctg attgccgatc tggacgccgg ttttgcgcgg
    16801 attgtataac attgccactt ttggacaatt ttgcagacat tttattgtga aaagtcttaa
    16861 attgttgcgt ccgggatcaa ggcgtcccgg acgattcagg agtacaatag gcagataaag
    16921 gcttaaacgc tgttccacag gaaagtccat ggctgttatt caagatatca tcgctgcgct
    16981 ctggcaacac gactttgccg cgctggcgga tcctcatatt gttagcgttg tttactttgt
    17041 catgtttgcc acgctgtttt tagaaaacgg cctgctgccc gcctcatttt tgccaggcga
    17101 cagcttgttg atattggctg gcgcattgat tgcccagggg gttatggatt ttctgcctac
    17161 gattgcgatt ctgaccgccg cagcaagtct gggctgctgg ctaagttata ttcaggggcg
    17221 ctggttaggg aataccaaaa cggtgaaagg ctggctggca cagcttcctg ctaaatatca
    17281 tcagcgcgcc acctgcatgt ttgaccgcca cggtctgctg gcgctgctgg ctggacgttt
    17341 tcttgcattt gtccgtacgc tgctgccaac catggcggga atttccggtc tgccaaaccg
    17401 ccgtttccag tttttcaact ggttaagcgg attgctgtgg gtcagcgtgg taaccagttt
    17461 cggctatgcc ttaagtatga ttccgttcgt taaacgccat gaagatcagg taatgacgtt
    17521 cctgatgatc ctgccaattg ccttgttaac cgctggcttg ttaggcacgc tgtttgtggt
    17581 gattaaaaaa aaatactgta acgcctgacg attttccctg ttcccggttg ctgtaccggg
    17641 aacgtattta attcccctgc atcgcccgca ttcttgccgc atcttccccc ggcgtcacac
    17701 cgaagtaacg tttaaactca cggctaaatt gcgatgcgct ttcatagccg acgcgcatcg
    17761 ctgctgcgct ggccttcatg ccgtcatgga tgatcatcat ccgcgcctta tgcagacggt
    17821 aattcttcaa atactgcaac ggcgaggtgc tggtgacaga cttaaaatta tggtggaacg
    17881 ccgatacgct catattggct tctgccgcca gttgctcgac gctcaggttt tcggtgtatt
    17941 tattctcaat ccgtttcagc acgcggctaa tcagactgaa gtgagtctgg cgactgacca
    18001 gcgccagtaa cgcgccgccg caaggtccgg tcagcacgta gtacagaatt tcgcggatga
    18061 tctgtttgcc gagaatacgc gcatccagtg gtcgctccat cacgtcgagt aaccgctccg
    18121 ccgcgcataa aatctcttct gataacgtgg cggagttaat cccgctggct gccatcgacg
    18181 gctggaaatg ctcatcttcg ccaatgtcca tcaacagttc ctgtaactgc aaaatatcga
    18241 cattgagacg caaccctgcc agcggcacct ctgacgtggc ataggtttcg cactcaaacg
    18301 gcaacggcac cgtcagcagc aggtattcat tggcatcata acgaaacacg cgttcattga
    18361 tataaccgat tttatgtccg gaaaagagaa ttatgatgcc aggctcgtac atcaccggtg
    18421 tacgtgcgaa aggcgtctcg ccatacaaca aacgcacatc gggcaacagt tctgacaaac
    18481 tattttcttt atttttcagt ttattaactt tatccgccag caagcggcaa atctcttcac
    18541 gtttcatatc gcgtaatttc ttaggaataa tgcggcaatt tgattgtgcg caattttgta
    18601 gcatttctcc agcactctgg agaaataggc aagacattgg cagaaatgag cattgagagc
    18661 cagggcgctg gcgatcacaa tgaaaaacat caggcagatc gttctctgcc ctcatattgg
    18721 cccagcaaag ggagcaagta atgaacaact ttaatctgca caccccaacc cgcattctgt
    18781 ttggtaaagg cgcaatcgct ggtttacgcg aacaaattcc tcacgatgct cgcgtattga
    18841 ttacctacgg tggcggcagc gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc
    18901 tgaaaggcat ggacgtgctg gaatttggcg gtattgagcc aaacccggct tatgaaacgc
    18961 tgatgaacgc cgtgaaactg gttcgcgaac agaaagtgac tttcctgctg gcggttggcg
    19021 gcggttctgt actggacggc accaaattta tcgccgcagc ggctaactat ccggaaaata
    19081 tcgatccgtg gcacattctg caaacgggcg gtaaagagat taaaagcgcc atcccgatgg
    19141 gctgtgtgct gacgctgcca gcaaccggtt cagaatccaa cgccggtgcg gtgatctccc
    19201 gtaaaaccac aggcgataag caggcgttcc attcagccca tgttcagccg gtatttgccg
    19261 tgctcgatcc ggtttatacc tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg
    19321 acgcctttgt acacacggtg gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg
    19381 accgtttcgc agaaggcatt ttgctgacgc tgatcgaaga tggtccgaaa gccctgaaag
    19441 agccagaaaa ctacgatgtg cgcgccaacg tcatgtgggc ggcgacgcag gcgctgaacg
    19501 gtttgattgg cgctggcgta ccgcaggact gggcaacgca tatgctgggc cacgaactga
    19561 ctgcgatgca cggtctggat cacgcgcaaa cactggctat cgtcctgcct gcactgtgga
    19621 atgaaaaacg cgataccaag cgcgctaagc tgctgcaata tgccgaacgc gtctggaaca
    19681 tcactgaagg ttccgacgat gagcgtattg acgccgcgat tgccgcaacc cgcaatttct
    19741 ttgagcaatt aggcgtgccg acccacctct ccgactacgg tctggacggc agctccatcc
    19801 cggctttgct gaaaaaactg gaagagcacg gcatgaccca actgggcgaa aatcatgaca
    19861 ttacgctgga tgtcagccgc cgtatatacg aagccgcccg ctaagctttt tacgcctcaa
    19921 actttcgttt tcgggcattt cgtccagact taagttcaca acacctcacc ggagcctgct
    19981 ccggtgagtt catataaagg aggaacgtat ggctaatcca accgttatta agctacagga
    20041 tggcaatgtc atgccccagt tgggactggg cgtctggcaa gcaagtaatg aggaagtaat
    20101 caccgccatt caaaaagcgt tagaagtggg ttatcgctcg attgataccg ccgcggccta
    20161 taagaacgaa gaaggtgtcg gcaaagccct gaaaaatgcc tcagtcaaca gagaagaact
    20221 gttcatcacc actaagctgt ggaacgacga ccacaagcgc ccccgcgaag ccctgctcga
    20281 cagcctgaaa aaactacagc ttgattatat cgacctctac ttaatgcact ggcccgttcc
    20341 cgctatcgac cattatgtcg aagcatggaa aggcatgatc gaattgcaaa aagagggatt
    20401 aatcaaaagc atcggcgtgt gcaacttcca gatccatcac ctgcaacgcc tgattgatga
    20461 aactggcgtg acgcctgtga taaaccagat cgaacttcat ccgctgatgc aacaacgcca
    20521 gctacacgcc tggaacgcga cacacaaaat ccagaccgaa tcctggagcc cattagcgca
    20581 aggagggaaa ggcgttttcg atcagaaagt cattcgcgat ctggcagata aatacggcaa
    20641 aaccccggcg cagattgtta tccgctggca tctggatagc ggcctggtgg tgatcccgaa
    20701 atcggtcaca ccttcacgta ttgccgaaaa ctttgatgtc tgggatttcc gtctcgacaa
    20761 agacgaactc ggcgaaattg caaaactcga tcaaggcaag cgtctcggtc ccgatcctga
    20821 ccagttcggc ggctaacgtg caaattctcc cggtggcggt aatgttccgc taccggactt
    20881 ttcagaaatc atttattccc ctcgcgtccc gcccgttgtt actcttcctt gttcaggaat
    20941 gccaaatata aggacatcat catgcagagc cggaagctct taaaagaaca actcatctat
    21001 atccgggata aacgcaacgg agaggtgaaa aacagatgaa aataatactt ctgtttttag
    21061 cagccctggc aagttttacc gtacacgcac agcccccctc acagaccgta gaacaaacag
    21121 tccggcatat ttatcagaac tataaatcag atgccaccgc cccttatttt ggtgaaaccg
    21181 gagagcgggc gataacttct gtgcgtattc aacaggcgct taccctgaac gacaatctta
    21241 cgctgccggg taatattggc tggctggatt atgatccggt ttgtgattgt caggattttg
    21301 gcgatctggt gctagaaagc gtagcgataa ccgaaactga cgccgatcat gccgatgccg
    21361 ttgtgcgctt tcgtatcttt aaagatgata aagaaaagac cacgcagaca ctgaaaatgg
    21421 tggcggaaaa tggtcgttgg gtcattgacg atattgtcag caatcatggc agcgtcttac
    21481 aagcagttaa tagcgagaat gaaaaaacgc tggccgcttt agcttcgttg caaaaagaac
    21541 agccggaagc ctttgttgcc gaactctttg aacatattgc cgattatagc tggccgtgga
    21601 cgtgggtggt ttccgactct taccgccagg cggtcaatgc cttctataaa accaccttca
    21661 agacggccaa caatcccgat gaagatatgc aaatagaacg gcaatttatt tacgacaatc
    21721 cgatctgttt tggcgaagag tcgctatttt cacgcattga tgaaattcga gtcctggaga
    21781 aaaccgccga ttccgcccgc attcatattc gttttacgct gaccaatggc aacaacgaag
    21841 agcaagaact ggttttacaa cggcgcgacg gtaagtggga aatcgctgat tttatccgcc
    21901 cgaacagcgg cagcctactt aagcagattg aggcaaaaac cgccgccaga ttaaagcaat
    21961 gagctgaatt aaataacaat tagccggaac aataaaaaag ggaacactat atgaaaacga
    22021 ttttcaccgt gggagctgtt gttctggcaa cctgcgtgct cagtggctgc gtcaatgagc
    22081 aaaaggtcaa tcagctggcg agcaatgtgc aaacattaaa tgccaaaatc gcccggcttg
    22141 agcaggatat gaaagcacta cgcccacaaa tctatgctgc caaatccgaa gctaacagag
    22201 ccaatacgcg tcttgatgca caggactatt ttgattgcct gcgctgcttg cgtatgtacg
    22261 cagaatgata aaaaaatccc cggcagcatg tcagttgccg gggatttttt ttaacgtcca
    22321 accgccgctt tagggcgttt cttcgcacca gcattcaccg gacgagattg cgtagacgac
    22381 gctttttttg ccgtagcagg cgtctgacgc tgggtcgcca tcggcgtatg tttcgtcaac
    22441 gctggacgag tattgcggtt ctgacgacga gcctcacgca tctcttcaat ggttggcgca
    22501 ggcactaagc aatcgcgacg gctgccaatc agatgctttt tacccattgc ttccagcgcc
    22561 tgacggatta acggccagtt tgccggatcg tggtaacgca acaacgcttt atgcaaacga
    22621 cgctgtttgt cacccttcgg tacaaagacg tcttcactct tataaccaat cttcgccagc
    22681 gggtttttcc cggtgtaata catggtggtc gagttagcca gcggcgatgg gtagaaattc
    22741 tgtacctggt cgagacggaa acgatgcttt ttcagccaca gcgccaaatt caccatatct
    22801 tcatcacgcg taccggggtg cgcagagatg aaatacggga tcagatactg ctctttacct
    22861 gcctgtttcg agtaagtatc gaacagctct ttaaagcggt catagctgcc catgcccggc
    22921 ttcatcatct tcgacaacgg cccttcttcg gtatgttccg gagcaatctt cagataaccg
    22981 ccgacgtgat gggtcgccag ttctttgata tagcgcggat cttccacggc gatgtcataa
    23041 cgtacgcctg aggcaatcag aatcttttta atgcctttca gatcgcgagc gcggcgatag
    23101 aggttgatcg tcggttcgtg gttagtgtcc atatgcggac aaatatccgg gtaaacgcag
    23161 gacaaacgac ggcaggtttg ttcagcgcgt ggcgatttgc agcgcaacat atacatgttg
    23221 gcagttggcc caccgagatc ggaaattacg cccgtaaaac ctggaacggt gtcgcggatc
    23281 gcttcgatct cattaatgat cgaatcttcg gaacggctct gaataatgcg cccttcgtgc
    23341 tcggtgatag aacagaaaga gcagccgcca aagcagccgc gcataatgtt gaccgaaaaa
    23401 cggatcattt cgtaagccgg aatacgggca ttgccatagg ccggatgtgg cacgcgcttg
    23461 tatggcagcg caaaaacgct gtccatctct tcggtagaaa gcggaatagc aggcgggttg
    23521 atccacacat agcggccgcc gtgtttttgc atcaatgcgc gggcacagcc tgggttggtt
    23581 tcgtggtgca gaatacgcga agcatgggcg tacagcactt tatcgccctt cactttctcg
    23641 aaagaaggca gcaacacgta ggttttttcc cacggtttcg ggcgcggtgg ctgcacggtt
    23701 acggctttgg cttcctgctt tttcggtgcc accggtttgt tatccgcgca cggcaaatct
    23761 tcgccatacg gatgcgggat cgggtcgatt ttcccagggg tatcaagacg agtagaatcc
    23821 acaccgctcc agcctggcaa cgcctctttt acgataatcg cagtattacg cacatcgcgg
    23881 atttcgctaa ttggctcgcc catcgccaga cggtgcgcca cctccaccag cggacgctca
    23941 ccgttaccaa acatcagcat gtcggctttc gaatccacca gcacggaacg gcgcacggta
    24001 tcggaccagt aatcataatg cgcggtacgg cgcagactag cctcaatacc accgaggatc
    24061 accggtacat ctttccacgc ctctttacaa cgctgggtat aaaccagtgt ggcgcgatcc
    24121 gggcgcttac ccgcgacgtt atctggcgtg taggcatcgt catgacgtaa acggcgatcg
    24181 gcggtataac ggttgatcat cgaatccatg ttgccagcag taacaccgaa aaacagattc
    24241 ggtttaccca gacgcatgaa atcgtctttg ctgctccagt ctggctgagc gatgatccca
    24301 acgcgaaagc cctgcgcttc cagcatacga ccgcaaatcg ccatcccgaa gcttgggtga
    24361 tcgacatacg cgtcgccagt aaccaaaatg atgtcgcagc tatcccagcc aagttgatcc
    24421 atctcttcac gagacatcgg caaaaacggt gccggtccaa aacaggcggc ccagtactgc
    24481 ggccatgaga acaggtcgcg atccggttgg atcagggaga tagagctcat tttgcttcca
    24541 gaaatgataa aaaaataatc aaaggccggg gattataagc cggaacgaaa gagaaatcga
    24601 aaagtattcc atactcgccc tcctcgggcg agtatgaaga ttacggtacc ggattgacta
    24661 acagttgccc aatcgagcca cggtccgcca tttccagcgt ctgactgttg aagtagaacg
    24721 ggaagtgcgc ccaggaaggc tgaccgaaat aaacaagcag ctccacttgt ccgtcaaccc
    24781 aaacggtatc tttccagcct ctgtcttccg ggaacggcat cgcaccattc acgttacgga
    24841 tctggaacat cacgccttca atatggaacg cttgcggctc gtccgcgcgt accgtccagc
    24901 gttcccacgt tccttgctgc gcggtgacat caatacggtt gacgtcccac agttgtccat
    24961 taatacccgg gtcatcaccc agactgatat cgcgactgcg aatcggcgaa ccggccatga
    25021 tttcagttgg cagcaagcgc atcggaagac tgtctgtgac cagcggcaga aggccggttg
    25081 ggcgcagcgt tagcaccagg gtagaaacca gaatgctgga tggctcaaag aagccacgaa
    25141 tacgatcaac aatgctcgcc gcttcgccac aggtgatcga cacttcatca ccgttgctca
    25201 tatccaccag aatctcgcgg cgctcgcccg gtgccagcga aagttgcttc accgatacag
    25261 gagcaggcag gaatccctga tcgccagaaa tcacatgtaa cgggcgacca tcgctcatct
    25321 gtagttgata gcgacgagag ttcgacgcgt tcagcagtcg caagcgcacc cagccacgcg
    25381 agacttcaac gtacgggctt tgtacaccgt taaccagcag cgtatcacca acaaagccgc
    25441 cgcttcccgg ttcgttgtat tctggcgtac caaagttatc cagccgttta tcctgaataa
    25501 tgaccggaaa atcatccaca ccataatggt tggggatagg cagcgacttg ctgacttcat
    25561 cttccaccag ccacattccg gcaaggccgt tatagacctg ctgagccgtg cggttgggag
    25621 tattggcgtg ataccacaga gtagctgcgt tctggcgaat gggcagtacg ggtgcccagt
    25681 cagcgtttgg cgacatcatc cgtgccggac cgcccatcag cgggcctggt acctgtagcc
    25741 cggccaccgt cattgagaca ttttctgtca ggcggttgct gtaaataagc ttaacatcgt
    25801 cgcccttcca gacgcggata gtcggcccca ggtaacgacc attgattccc cagaccgacg
    25861 cgcgtgtccc tggcgtaaat gaccagtgcg cacgttgtac agtcataaac agcggttgcc
    25921 cacggcgaga ttcaagcagc ggcggaacgg gtagcggttg ctgttgcccg gctgcgctgg
    25981 ccttcagggg aacagcgcct gcacaaagtg caatccccga tgcctgaatg aactgacgcc
    26041 gactgagtga catatttgct ccatgtaaaa ctggctaaag taataccgtg caggaaagtg
    26101 ttccccgcaa aaatacagat tgccgactta aacttttccg gcggcttcgc gttctgcgac
    26161 ttctttatcg agctcggcga ttttttgttc cattatcgaa cgacaatggg cagccagctc
    26221 acgaacctga tctttgccat actgactgac gtcaattggc ggcagcattt cgacaatcac
    26281 cagaccgttg tgcagtcgat taagattaat cttattcgaa gttgtagaga cgcacacggg
    26341 aataatcggg acgcctgctg caattgccgc gtgaaatgct ccagtcttga acggtagcag
    26401 gccgcgacca cgactgcggg ttccttccgg gaacatccaa atggaaatac ggcgtttttt
    26461 gaaatgattc actacttccg caatagtgcc gtgagcttta gtgcgattgt ttctgtcgat
    26521 caataagttg ccggttaacc agtacaactg cccgaagaaa gggatccaca gcaagctctt
    26581 tttacctacc gtcaccgtcg gcggttgcac aatgttcgat gctgtcacca tgtcatagtt
    26641 gttctggtgg ttagcgatat agatagcatt gccgtagctt tcagcatctg caggtttgcg
    26701 gcactcaact ttcaggccga acagcggcgc aagacgacca aacatatgcc caaaggtggc
    26761 cacatgtttc gggttacgcg ggctgaaaag gcagtaaatg gagccgaata cacagactaa
    26821 gatgctgtaa atcacggtaa taataagacg aaagatatat agcataacac cctctgaagg
    26881 tctgagagac tggcattgta cgtcacctga aatcaggtta gggcattgtt aaaagccgct
    26941 ctctggaaaa gcgtattgtt aatcgcaacg catgaataaa caacggtttc aagggaattt
    27001 ttatcgtttc cgggggttga atacgctgcc ggatgacgac ttaacgtttc atccggcgtt
    27061 ccttgcaagc gggaggaaac agcgccctcc ccggcatatt actcttcgct atcaccgctg
    27121 ctggcacggc gaggagagtc gatctcaaca cgatcgatac gctgcaaacc gcgcatcaac
    27181 gtaccgcggc gtccacgttc gccagtgact ttctgtaact cttccgggcg cagtttaatt
    27241 ttgcgtttcc caacatgaat ggtcagcgtg ctttgcggcg gcagaacgta cagttgcgcc
    27301 agaccatctt ctccacgcgc ggcttctgcc gatggaatgt tgataatctt gttgcctttg
    27361 cccttcgaca gctgcggcag atcgcttacc gggaacatca acatacggcc tgcctgagtg
    27421 attgccagca gcatatcgga agcatcttca attaccaccg gcggcataac atgggcattt
    27481 tccggtaagg tgatcaaagc cttacctgca cggttacgcg ccaccagatc gttaaaggtg
    27541 cagacgaaac cgtaacccgc atcggaagcc atcagcagtt tctgatcgtc gctttccatc
    27601 agcatatggt caacggtcgc cccaggcggc aacgttaatt tgccggtgag cggttcgccc
    27661 tgaccacgcg ccgacggcag cgtaatcggg tcaatggcat agctacgacc ggtggaatca
    27721 acaaacacta ccggttggtt gctcttacct ttcaccgccg ctttgaagct atcacccgct
    27781 ttataattca ggcccggcgc gtcgatatca tggcctttag cgctgcgtac ccagcccatc
    27841 tgcgacagca caatggtgac aggttcagac ggcagcatgt cgtgctcgct catcgctttc
    27901 gcttcttcgc gttcctgcaa cggcgaacga cgatcgtcac cgtaggcttg cgcgtctgcc
    27961 tgcagttctt tcttcagcag gttattcatt ttacgctcgg aagccaaaat gccctgcaac
    28021 tggtcgcgct ctttttccag ttcactctgc tcaccgcgaa tcttcatctc ttccagtttg
    28081 gcaagatgac gcagtttcag ttcgaggatc gcttccgcct gggtttccgt aaggccaaac
    28141 cgcaacatca gcgccggttt cggttcatct tcattacgaa tgatctcaat cacttcgtcg
    28201 atattgagaa acgccaccag caaaccttcg aggatatgca ggcgcttgag gactttctcc
    28261 agacgatagt tcagtcggcg gcgcacggta tcgcggcgga acaccagcca ttcggagaga
    28321 atttccagca ggtttttcac cgccggacga ccatccagac cgatcatatt gaggttaatg
    28381 cgatagctct tttccagatc ggtggtagcg aagaggtggt tcatcacctg atccatatcc
    28441 acgcggttgg aacgcggcac aatcaccaga cgggtcgggt tctcgtggtc agattcatcg
    28501 cgcagatcgt caaccatcgg cagctttttg ttgcgcattt gcgcagcaat ttgctccagt
    28561 acgcgcgcac ctgaaacctg atgcggcaat gcgctgataa ccaccgcgcc atcttctttc
    28621 ttccacaccg cacgcatacg cactgaacca cgtccgttct cgtagatttt acggatctcg
    28681 gcgcgcgaag tgataatttc cgcttcagtc ggataatccg gcccctgcac gatatccagc
    28741 agctgatcga gcgtggtttt cggctggtcg attaatgcga ttgccgcctg agccacttca
    28801 cgcaggttat gcggtggaat atcggtcgcc atgccgacgg caataccggt ggtgccgtta
    28861 agcaaaatgt ttggcagacg ggcaggtagc attttcggct cctgcaaagt gccgtcgaag
    28921 tttggcaccc agtcagccgt cccctgcccc aactcgctca atagcagctc ggaatatttc
    28981 gacaaccggg attcggtgta acgcattgcc gcgaacgatt tcggatcgtc cggcgcgccc
    29041 cagttcccct gaccatcaac cagcggataa cggtaagaga acggctgcgc catcaggacc
    29101 atcgcttcat aacaggcgct atcgccgtgc ggatggtatt tacccagtac gtcaccgacg
    29161 gtacgggccg attttttaaa tttggcgctg gcattcaggc ccagttcaga catcgcatac
    29221 acaatgcggc gctgaacagg tttcagacca tcaccaataa acggcaacgc acggtccatg
    29281 atcacgtaca tggagtagtt taagtaggcg ttttccgtaa attcatgtag cgcaaggcgc
    29341 tctgccatat cgctcattaa ttctgattcc tcaacttatt cgccagcctt caaaggggca
    29401 atattgccgg agatactaca ttatctgccg cgtcgagtca caggagatca gcgtgaatag
    29461 cgccgccgga cgtgctccga cggcgataaa ggcatcaatg tgccttgata tacaactctt
    29521 tgctgtagac ataatcctgg ggattatgca gcggaaaacc gccaacgtag ggtttaagca
    29581 gtttgattaa cggctgatag tagataggaa tcagcggtgc ctgttggttg atgatcactt
    29641 ctgcctgctg atacaacgca ttacgctttg tcgcatcagt gatctgcgtg gcctggttta
    29701 gtaaggcgtc atactgcgca tttttccagt gaccgacgtt ttcttcacta tcgcttttga
    29761 gcgtgttcag gaagctggaa gcatcattgt acgtcgcatc ccacgactgc cgagacagca
    29821 tgaaatcacc ggctcgtcgg gcatcaagat aggttttcca ctccattgtg cgcagcgtca
    29881 cctgtgcacc cagccatttt ttccattcgg aagacaacgc tatcgcggtc ttttcatgca
    29941 gatcgtactt gttgtagaac agctcaaagc gtagcggatg agaggcgtcg tatcccgcct
    30001 gtttcagcaa ggcttttgcc atcgcgacgc gctcactcat tggcttttgc agttcatcga
    30061 acgtcgtcgc gctaaagcct tttacctctg gcggcgtcag cgtggttgcg ggcgttctca
    30121 accccagtac cttttgcgca ataagctgtc gatcaaccgt aagatatagc gcccgacgca
    30181 cccgcacatc gttaaatggc ggtttctcaa ggttgaagtt gtaatattcg ctgttcagac
    30241 gcggaataat tcgtagctcg ccaggcagtg atttttcaat ggcgggaatt tgctgcgccg
    30301 gaacccaggt gagatcgacc tctcccgcgc gatagcggtt atagccggtg accgaattat
    30361 ccagcgccag atactcaacc tgttgcaata ttgtatgttg cgcatcgcgg tactttggat
    30421 ttttgcgtgc agtaatcttt tcgttaacta cccactgatc aagcacaaag gcaccgttgt
    30481 aaaccatgtt ctctggctta ctccagctat cgccatgttt agcgatgaca tgatgaggaa
    30541 ccgggaacag cgtcggccag gcgagcatcg tcgtgaacca aggaaccggc tgctcaagcg
    30601 taacttcaag agtacgatca tccgtcgctt tgacacccag cgatgtaaca tctgctttac
    30661 ccgcaacaat agccgcggca ttgttaatgt gcgcctgtgc cagatagcca gcaaaagggc
    30721 ttgccgtttt cgggtcaacc gcgcgctgcc agccgaggac aaaatcctct gccgtcagag
    30781 gctgaccgtc ggaccactgc aaaccgctac gcagatggaa aatatagcgc ctgccgccgt
    30841 ccagtatctc ccagcgttca gcctgagcgg gctgcacctg gccttcacca tccatccata
    30901 ccagaccttc aaacagatcc agcacaatct gcgcggcagt attctcctcc accttttgcg
    30961 ggtcgagcgt acctgggtcg ctatgattgt tgtaacgaaa gacttgttgc ggagcgagcg
    31021 gtgtgttggt gggaacgtca gcagcgtaga gaggagccgc acttaccagg ctgaccagcc
    31081 ataataaatt tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa
    31141 tacgcctgtg gcattgttcg ctcaaactta tcacgagtga tttggctcac actccggtaa
    31201 agattcccca tcacgctaac ggcacataaa catccgtttc cagtcgctgc tctgtcaccc
    31261 cttcggcaag attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg
    31321 caggcagcca gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa
    31381 cgtggcgggc cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat
    31441 tatcgggaat cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg
    31501 tggtggcagg gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc
    31561 ccgtctcttt acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac
    31621 agcggtgcgg caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg
    31681 tcaggtttgt cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt
    31741 aacaattacg gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct
    31801 ttatcgactt caccctgaat ctcaaccgta tctttcggcg tcaccgtcac gccgttccag
    31861 cgtttgtgat cgatatcaac attgatagta ccgctggcat ctttgaacac gtagagatcg
    31921 tcagagatgc gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat
    31981 tttgcgcttt ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt
    32041 tgcgttgccg atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcgctgcac
    32101 agggccatta ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc
    32161 tgtttccgag catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt
    32221 tagtgctgta cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg
    32281 gatgaaaaaa tgcgagtttt actgatagaa gatgacatgc tgattggcga cggcatcaaa
    32341 acgggcctta gtaaaatggg ttttagcgtc gactggttta cacaaggtcg tcagggaaaa
    32401 gaggcgcttt atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg
    32461 gatggtcgcg atattttgcg cgaatggcga gaaaaaggtc agcgtgagcc ggtactgatc
    32521 ctgaccgcgc gcgatgcgct gggggaacgt gtagaagggc tgcgtctggg agctgacgat
    32581 tatctgtgta aaccttttgc gttgatagaa gtcgccgcca ggctggaagc tctgatgcgc
    32641 cgaaccaacg gccaggccag caacgagctg cgccacggta acgtcatgct cgaccccggc
    32701 aaacgtatcg ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg
    32761 ctggaattac tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa
    32821 ctgtatacct gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg
    32881 cgacgcaaac tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt
    32941 gagaaatgaa atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa
    33001 ttctggcctc ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata
    33061 acgtcgatga attgttcgac acccaactga tgctttttgc caagcggtta agtacgctcg
    33121 atctcaacga aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg
    33181 gtcatgttga tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc
    33241 ttaacgatgg cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg
    33301 acgggcaact ggtcggtgaa gacgatccct ggcgttttgt ctggatgacc tcacctgatg
    33361 gcaaatatcg catcgttgtt ggtcaggaat gggaataccg tgaagacatg gcgctggcga
    33421 ttgttgccgg gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga
    33481 tggtactact gggtcgtgaa ctcgcgccgc tgaacaagct ggcgctggcg ctacgtatgc
    33541 gtgaccctga ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac
    33601 tggttgagtc gctaaatcaa ctgttcgtcc gcacacatgc gatgatggtt cgtgaacgac
    33661 gctttacctc cgacgcagct cacgaacttc gtagcccgtt aacggcgctg aaagtgcaaa
    33721 ccgaagttgc gcagctctct gacgatgatc cgcaggcgcg aaaaaaagca ctgcttcaat
    33781 tacattacgg gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc
    33841 tggactcact ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat
    33901 cgtcggtgat ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac
    33961 tcaatgtcca gggcatcaaa cgcaccggac aaccgctatt gctaagtttg ttggtgcgaa
    34021 atttgctgga taacgccgtg cgttacagtc cacagggcag cgtggtggac gtcacgctga
    34081 atgccgataa tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc
    34141 gaattggcga acgcttctat cgcccacccg gacaaaccgc taccggcagt ggacttgggc
    34201 tatcgattgt ccagcgaatc gccaagctgc atggcatgaa tgttgagttt gggaatgcgg
    34261 aacaaggtgg atttgaggcg aaggtaagct ggtaacttct ctgtctatga gagccgttaa
    34321 aacggctctc atagatttta ctaatagcaa aatataaacc gtccccaaaa aagccaccaa
    34381 ccacaaacca gacaaataac gaacgagcat acatataacc aaaactcaaa catttcccgc
    34441 acaaagagtt ttcttaagat aataataata agtggcgtca gaagaaaaaa tgctgcaatc
    34501 attgcttgca atatacgatc ttcgcctgag acggaaggta tactgcaaat attgtctccc
    34561 gagtcaagca tccagtcata cttattgaaa gcaaaaaaag catctattaa ccaataagta
    34621 agcattatat ataacagagc ttcaaatatt gtttgaattt catgctttaa catatgttag
    34681 cactctctaa aaaagtccag tcggagatct ggttttcgat accctccagt aaatctattt
    34741 aaaataatct gcgtaacacg ctgatatttt catttttagt tattcaacac cacaattatc
    34801 aactcgcaga aagtccacac caacgttgta aattaaccct gtgctagttg gaatgatgag
    34861 aaattgctat cagtattgtc attttcagaa aatatttatc ctgcatcggt gagtcagagc
    34921 aagatcagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat ttcaccgtac
    34981 cgctctgtga cgtactatag tcggcaaacg tctcaccttg aggttaaaaa atgagcaaca
    35041 tcctgattat caacggcgcg aaaaaattcg cccactccaa tggtcaactg aacgacaccc
    35101 tgaccgaagt cgcggatggc acactgcgcg acctcgggca tgatgttcgc atcgttcgcg
    35161 ccgacagcga ctacgatgtc aaagcggaag tacaaaactt tctctgggct gatgtggtga
    35221 tctggcagat gccaggctgg tggatgggcg cgccgtggac agtgaaaaaa tacattgatg
    35281 atgtattcac cgaaggtcac gggactctgt atgccagcga tggtcgtacc cgcaaagatc
    35341 cgtcgaaaaa atacggttcc ggcggcctgg tacagggcaa aaaatatatg ctttctctga
    35401 cctggaacgc gccaatggaa gccttcaccg aaaaagatca gttcttccac ggcgttggcg
    35461 ttgacggtgt gtatctgccg ttccataaag caaaccaatt cctcggtatg gaaccgctgc
    35521 cgacatttat cgctaatgac gtgataaaaa tgcctgatgt tccccgctat actgaagaat
    35581 atcgcaagca tcttgtggaa atttttggtt aactagagct caggctttag aaggagttaa
    35641 ccatgcttac cgtaatcgca gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcgg
    35701 tattggatca gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt tgccacggct
    35761 atgcgccaat ggtggattgc gctgctggcg tgagtttcca gtctatggca ccggattcta
    35821 tcgtgatgat tgagcagtgg gaaagcatcg cgcatcttga agcgcatctg caaaccccgc
    35881 acatgaaggc gtatagcgaa gccgtaaaag gtgacgtgct ggagatgaat attcgtattc
    35941 tgcaaccggg gatttaaacc tgccttgttt gcccggccat cctgaccggg caatgttctt
    36001 tcctttaaac ctcaatctcc gccatgtcgc ctttctcttg caaccaattg cggcgatctt
    36061 ccgagcgttt cttcgccagc agcatatcca tcatcgcgtc agtacgctga tcgtcttcat
    36121 catcgatagt cagctgcacc agacggcgag tgttcggatc aagcgtggtt tcgcgcaatt
    36181 gcatcgggtt catttccccc agacctttaa aacgctggac gttcggcttg cctttcttgc
    36241 gttttaattg ctcaagtacg ccctctttct cttcttccgt cagcgcgtaa taaacctctt
    36301 tcccgagatc aatacggtag agcggtggca gtgcgacgta aacgtgaccg tgtttcacca
    36361 acgcgcggaa gtgttttacg aacaaagcgc agagcagcgt ggcaatgtgc agaccatcag
    36421 agtccgcatc cgcgaggata cagattttgc cataacgaag ctggctcagg tcgtcgctgt
    36481 caggatcgat accgatcgct accgaaatat catgcacttc ctgcgaggcc agcacttcgt
    36541 cggaagaaac ttcccaggtg ttaaggatct tacctttcag cggcatgatc gcctgatatt
    36601 cacgatcgcg cgcctgcttg gcagatccgc ctgcggagtc accttccaca aggaacagtt
    36661 cggtacggtt aagatcctgc gcggtacagt cagccagttt gccaggcagc gccgggccgc
    36721 tggtcagctt tttacgcacc acttttttag ccgcacgcat acgacgctgg gcgctggaaa
    36781 tcgccatctc tgccagcaac tccgccgcct gaacgttctg gttcagccac aggatgaagg
    36841 catctttcac cacgcccgaa acgaatgccg cacactgacg cgaagagaga cgctctttgg
    36901 tctgcccggc aaactgcgga tcctgcattt ttactgacag cacataggcg cagcgatccc
    36961 agatatcttc cgccgacaac tttacaccgc gcggcaaaat gttgcggtat tcacagaact
    37021 cacgcatcgc gtccagcagg ccctgacgca ggccattaac atgggtaccg ccctgcatcg
    37081 ttgggatcag gttgacgtag ctttcggtca gcagttcacc gccttccggc agccacagta
    37141 gcgcccagtc caccgcttca gtatcgccag cgaaattacc gataaagggt ttttctggca
    37201 gcgtcggtaa accgtttacc gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc
    37261 agcgctgttc ggtgttgttg atctcatctt taaaggtaat ctcaacgccg gggcacagta
    37321 ctgctttggc tttcagcaca tgcgtcaggc gtgaaacaga aaaacgcggg ctatcgaaga
    37381 aggtttcatc cggccagaaa tggacgctgg tgccggtatt gcgtttaccg caagtgccga
    37441 caacctgtaa atcctgcacc ttttcgccat tttcaaaggc gatgttataa acctgaccat
    37501 cgcggcgcac gttaacttct acgcgcttcg acagggcgtt aaccaccgaa atccccacgc
    37561 catgcaggcc gccagagaac tggtaatttt tgttagagaa tttaccgcct gcatgcagac
    37621 ggcaaagaat cagttcaacc gccggtacac cctcttccgg gtgaatatcc accggcatcc
    37681 cgcgcccatc gtcaataact tctaacgact ggtcagcatg taaaataacg tccacgcgtt
    37741 ttgcgtgacc cgccagtgct tcatccacac tgttatcaat gacttcttgc cccaaatggt
    37801 tagggcgagt ggtatcggta tacatccccg gacggcggcg aaccggctca agcccggtga
    37861 gtacctcaat ggcatcagcg ttataagttt gcgtcatggt ttaagttagc aattcgagtt
    37921 gatcgtcaga gatggtgcag accaagaaaa tcgacgatcg ggttgaaata atcttcgaag
    37981 cccgtgaatg cgtggttgcc gccttctatg acagtctggc ggcaggaagc gtagtacgcc
    38041 accgcctggc ggtaatccag cacttcatct cccgtctgtt gcagcagcca gatcaaatcc
    38101 ggcgcttcca gcgggtcaat ctgcatgact ttaagatcgt aaatatggcg tgactctagc
    38161 acatattgct gcccggtgta ggggttctcg ttctgaccga gatagtccgt cagcagttca
    38221 aacgggcgca ccgccgggtt taccaccact gcgggcagca taaaacattg tgacaaccag
    38281 gtggcgtaat atccccccag tgacgaaccg acaataccca gcgaatcgcc gccatgttcc
    38341 aggacaatgg attccagcag ctctgccgcg tcggaaggat acggcggcaa ctgcggaatg
    38401 atcatctcaa cgtcagggtg atgttccgcc agccagtttt ttaacaagct cgcttttgca
    38461 gagcgcggcg agctgttgaa accgtgtaaa taaagaagcg tagacatcag tagccttctg
    38521 aagcggtatc aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc
    38581 catcagcatg taactcgaga gtacgccagc cgggcgcgat agtatccagc gtaaagttgg
    38641 aacagtgcgg cttaaactgc acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc
    38701 agtcgagatc cagctcctga tgaatatgac cgcacagcaa gtatttgacg tgcggaaact
    38761 tcgccagcac ggtatccagt tcgcccgcgt tacgcaggct gtgttgatca agccaactac
    38821 aacccgcagg tagcggatga tgatgcagca gcagcaacgt atggcgctct ggcgcatcgg
    38881 ccagtttacg ttccagccac tcaagctgaa actcgcttag ctcaccgtgc ggcacgccaa
    38941 acacctggct atccagcaac aggatttgcc attgctcacc aataaacacg cgcttcgccg
    39001 gggagatacc cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg tggttgcccg
    39061 gcagccagac gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc tgataggccg
    39121 cagaggattg atcctgcgct aaatcacctg tcgcgacaat caggtcgaat tcgtgctggt
    39181 gtggacgaat cgcctccagc accgcctggt agctttccca ggtgtttacc cctaacaggg
    39241 cttcgtgctt ttgtgcaaac aggtgagtat cggtaatttg taaaatcctg actctggcct
    39301 caccagccag aggaagggtt aacaggcttt ccaaatggtg tccttaggtt tcacgacgct
    39361 agtaaaccgg aatcgccatc gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa
    39421 actggttaat ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga taatcatacc
    39481 gcgctttgaa gcgaaaaatc tgctggcttg aacacacttc agccaccatc gcgtcatgat
    39541 acagacgcac cgtcattgac ggaaggctcc agtaactgat cgcgggcgca gtctgttcta
    39601 ttgtcaccag ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg
    39661 ccacctgata gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt
    39721 gtgaaaagtt catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca
    39781 tttataccca ctcatttttt aacgcttgat gatgcagctg cagccattgc aaagcgatga
    39841 ccgacgctgc gttgtcgatt ttcccctctt ctacccactg gtatgcctgt tcccggctta
    39901 ccacatgaac gcgaatatct tcgttttcat cagccagacc gtgaataccg cttgcggtcg
    39961 tggcgtccac ttcgcccacc ataattgacg aacgctcact ggtgcccccc gggcttgcca
    40021 ggaaacttaa caccggtttg gtccgtttga ctatcagccc cgcctcttca atcgcttcgc
    40081 gacgggcaac atcttccaca ctttcaccct cttctatcat cccggcaacc atctccagta
    40141 gccagggggt ttcgctagtg tcgtacgcgg caattcgaat ctgctcaatc agcacaactt
    40201 catcacgcac tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc
    40261 gccgtacctc atgactcatt tgcccgttga atagacgatg acgaaatcta taaagatcta
    40321 atgaaaaaaa gccgcgataa agtgtttctc gtgcaataat ttctacatcg tttttgccaa
    40381 atgtaacggg caggttgtct ggcttaagca ttgttaatgt cctggcacta atagtgaatt
    40441 aaatgtgaat ttcagcgacg tttgactgcc gtttgagcag tcatgtgtta aattgaggca
    40501 cattaacgcc ctatggcacg taacgccaac cttttgcggt agcggcttct gctagaatcc
    40561 gcaataattt tacagtttga tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa
    40621 gaaattgctc cccattctta tcggcctgag cctttctggg ttcagttcgt tgagccaggc
    40681 cgagaacctg atgcaagttt atcagcaagc acgccttagt aacccggaat tgcgtaagtc
    40741 tgccgccgat cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc
    40801 acagctaggt ttaggtgcag attacaccta tagcaacggc taccgcgacg cgaacggcat
    40861 caactctaac gcgaccagtg cgtccctgca gttaactcaa tccatttttg atatgtcgaa
    40921 atggcgtgcg ttaacgctgc aggaaaaagc agcagggatt caggacgtca catatcagac
    40981 cgatcagcaa accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat
    41041 tgacgttctt tcctatacac aggcgcaaaa agaagcgatc taccgtcaat tagatcaaac
    41101 cacccaacgt tttaacgtgg gcctggtagc gatcaccgac gtgcagaacg cccgcgcgca
    41161 gtacgatacc gtgctggcga acgaagtgac cgcacgtaat aaccttgata acgcggtaga
    41221 gcagctgcgc cagatcaccg gtaactacta tccggaactg gcggcgctga atgtcgaaaa
    41281 ctttaaaacc gacaaaccac agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa
    41341 cctgtcgctg ttacaggcac gcttgagcca ggacctggcg cgcgagcaaa ttcgccaggc
    41401 gcaggatggt cacttaccga ctctggattt aacggcttct accgggattt ctgacacctc
    41461 ttatagcggt tcgaaaaccc gtggtgccgc tggtacccag tatgacgata gcaatatggg
    41521 ccagaacaaa gttggcctga gcttctcgct gccgatttat cagggcggaa tggttaactc
    41581 gcaggtgaaa caggcccagt acaactttgt tggtgccagc gagcaactgg aaagcgcgca
    41641 tcgtagcgtc gtgcaaaccg tacgttcctc cttcaacaac attaatgctt ctatcagtag
    41701 tattaacgcc tacaaacaag ccgtggtttc cgcccaaagc tcattagacg cgatggaagc
    41761 gggctactcg gtcggtacgc gtaccattgt tgatgtgttg gatgcgacca ccacgctgta
    41821 caacgccaag caagagctgg cgaatgcgcg ttataactac ctgattaatc agctgaatat
    41881 taagtcagcc ctgggtacat tgaacgagca ggatctgctg gcactgaaca atgcgctgag
    41941 caaaccggtt tccactaatc cggaaaacgt tgccccgcaa acgccggaac agaatgctat
    42001 tgctgatggt tatgcgcctg atagccctgc acccgtcgtt cagcaaacat ccgcacgcac
    42061 taccaccagt aacggtcata accctttccg taactgatga cgacgacggg gcttcggccc
    42121 cgtctgaacg taaggcaacg taaagatacg ggttatctgc cgcattcttc ccccttctcg
    42181 cttcaatttc gaccagccat cctctattct gatgggtatt taccactggt cccggaagac
    42241 aaaaatgaaa cggacaaaat ccatacgcca cgcatcgttc cgcaaaaact ggagcgcacg
    42301 ccatctgaca ccagtcgctc tcgcagttgc cactgttttt atgctggctg gctgtgaaaa
    42361 gagtgatgaa acagtgtctc tctatcaaaa tgctgacgac tgttcagctg caaacccagg
    42421 caaaagcgcc gaatgtacca ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc
    42481 gccgaaatac gccacccgtg aagactgtgt tgctgaattt ggtgaaggtc agtgccagca
    42541 ggcaccagcc caggctggca tggcaccaga aaaccaggcg caggcccagc aatccagcgg
    42601 gagtttctgg atgccgctga tggccggtta catgatgggt cgtctgatgg gcggcggcgc
    42661 gggatttgca cagcagccgc tgttctcctc gaaaaaccca gccagcccgg cttacggtaa
    42721 atataccgac gcgacgggta aaaactatgg cgcagcccag ccaggccgca ccatgaccgt
    42781 accgaagaca gccatggcac caaaaccggc gaccaccact accgttaccc gtggcggttt
    42841 tggtgaatct gttgccaaac aaagcactat gcagcgtagc gcaaccggta cctcttctcg
    42901 ttcaatgggt ggctgatacc gatggaaaga gtcagtatta ccgagcgccc ggactggcgt
    42961 gagaaagccc acgaatacgg tttcaatttt cacaccatgt acggcgagcc gtactggtgt
    43021 gaagatgctt actacaagtt gaccctcgcc caggttgaaa agctggaaga agtcaccgcc
    43081 gaactgcacc agatgtgcct gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg
    43141 accaaattcc gcattccaaa acacacctgg agttttgtgc gccagtcatg gctgacgcac
    43201 cagccatcgc tttattcgcg tcttgatctg gcgtgggatg gcactggcga acctaaactt
    43261 ctggaaaata acgccgatac gccaacgtca ctatacgagg cggcgttctt tcagtggatc
    43321 tggctggaag atcagcttaa cgccggtaac ttgccggagg gcagcgacca gtttaacagt
    43381 ctgcaagaaa aactgatcga tcgcttcgtt gagctgcgtg aacagtatgg cttccagttg
    43441 ctgcatctca cctgctgtcg cgacacggtg gaagatcgcg gtaccattca gtatttgcag
    43501 gactgcgcga cggaagctga aatcgctacc gagttcctct acattgacga tatcgggtta
    43561 ggtgaaaaag gtcagttcac ggatttacag gatcaggtaa tttccaacct gttcaagctg
    43621 tatccgtggg aatttatgtt gcgtgagatg ttttccacca agctggagga tgcaggcgta
    43681 cgctggctgg aaccggcgtg gaagagcatt atctccaaca aggcacttct accgctactg
    43741 tgggagatgt tcccgaatca cccgaacctg ctgcccgctt attttgcgga agatgatcat
    43801 ccgcaaatgg aaaaatatgt ggttaaaccg atcttctccc gtgaaggcgc aaacgtgtcg
    43861 atcattgaga acggcaaaac cattgaagca gcggaaggtc cgtatggcga agaagggatg
    43921 attgttcagc aattccaccc gttaccgaaa ttcggcgaca gctatatgct gattggtagc
    43981 tggctggtga acgatcaacc cgccggaatt ggcattcgtg aagaccgtgc attgatcacc
    44041 caggatatgt ctcggtttta tccacatatt tttgttgaat aatcaatgat gccggatggc
    44101 attcgccatc cggtaattgt tagcctatct gcaccgacag catacttaaa ctgcccattt
    44161 ctataccatc gaccggaatg gtaattggct cctgcccatc ccacgcacct aacacataca
    44221 acaacggcag atagtgctct ggcgttgggt tcgataacgc gccaccttca tggtcgagat
    44281 aattcaccag aggatgctgc tctactggcc cttgccacgt cagattcgct ttcacatact
    44341 cattaaacga cgtcgcccac ggatacggtg aactatcacc gtgccacttc actgtacgca
    44401 ggttatgcac cacgttgccg ctggcgacca acattattcc ttcatcacgc agcgccgcga
    44461 gttttcgccc catttcgaaa tgccaggcgg caggtttgct actatcgata ctcaactgca
    44521 ccatcgggat atcggcgtca ggatacatct taatcagcac gccccaggaa ccgtggtcaa
    44581 agccccaggc ttctttatcc agcgtcaccg ggaccggcgc taacagctca accagatgct
    44641 gtgccagcgc aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc
    44701 caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa
    44761 accagtgagc cgaaactacc acaatcgctt gcgggcgtgg caatgtcatc cccaacgtct
    44821 gccagctgcg ggtatacaaa ttatcttcca gcacgttcat cggactaccg tgacctaaaa
    44881 acaatgctgg catacgtgtt gaagacatga tgatatcctt aactaaaggt gtcattttga
    44941 tatcctcaca atacgcttgt tcggcggagt aagaacccgg ataacaatga tgatgatcat
    45001 cagttatttt gacgatctgc ctgaaggtga agatttataa ggagttgtcg atgtcagtac
    45061 ctctcattct gaccatactg gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc
    45121 tcgggcaaaa accctcgaac cgcttactgg cgttttcgct aggatttgcg gcggggatca
    45181 tgttgctcat ctcattaatg gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc
    45241 ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg ctattttggc ctggaccgca
    45301 tgttgccaca tgcgcatccg caggatttaa tgcaaaaatc ggtgcagccg ttgccaaaat
    45361 cgatcaagcg cacagccatt ctgctcactc tcggcatcag tctgcataac ttcccggaag
    45421 ggattgccac ctttgtcacg gcgagcagca acctggagct gggatttggc atcgctctgg
    45481 ccgtagcgtt gcacaatatt cctgaaggtc tggcagtggc aggcccggtt tacgcagcaa
    45541 cggggtctaa acgcacggcg attctgtggg cggggatttc tggactagca gaaattcttg
    45601 gtggcgtgct ggcgtggtta atcctcggta gcatgatttc cccggtggtc atggcagcaa
    45661 tcatggcggc ggtcgcagga attatggtgg cgctctcggt tgatgaatta atgccgctcg
    45721 ccaaagagat tgaccctaat aataacccca gctatggcgt actatgtgga atgtcagtga
    45781 tgggattcag tttggtgctg ctacaaacgg cgggaattgg ttaaaaacaa catcagaagt
    45841 tgtccagcgc atggacaact tctgctagtt tcactaaagc cttattctac cttttcggct
    45901 gtctcttctc tcgtactgtt taagtatttg ttccgcgctt caagcagtgc gtctctgtcc
    45961 tggcgaaatg cccgacaata acgtttcaaa tgcaggaaat aacccaccac aaccagaaca
    46021 atcaatacaa tccagtacca ggcgataaac atccttttac ccttaatatt taacaatact
    46081 aaacatttag cgcataaatt tcacatatcc tttttcggat atattcatat ggtcgcataa
    46141 taacggaaca attatatgat tttgatcata atgaaaaaga atgcctgata ttaatgaaat
    46201 tttctcatag aaagagaagc ggaattaaca tcttgcatag cagggaaaaa taaaaaccga
    46261 agcctgctac aacttcggtt tcttgattaa ggcagtaaat taagcagcgg ttttcagctg
    46321 gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac aagcgccata
    46381 ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta
    46441 gtcagctcac acagcacacc agccggtttg aagcctgcca gcgtcattag atcaatagtt
    46501 gcttcagtat gaccgccacg cgtcagtaca ccaccagcct gagcgcggag tgggaaaacg
    46561 tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc
    46621 gtaatacggt cagcggcaga aacaccggta gtcacacctt cagccgcttc aatggtcacg
    46681 gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag atcgagttgt
    46741 ttacggcgat cttcagtaat gcacaggcaa acaataccac taccgtggcg aatggtcagc
    46801 gccatctgct caacagtcat ggtttctgcc gggaagatca tatcaccttc gttttcacgg
    46861 tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag tgcattttca
    46921 acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt aaaaaaacct
    46981 cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat atagccaaaa
    47041 taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg actctaaccg
    47101 tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg
    47161 gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg agaataagcg
    47221 gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata attctggttt
    47281 atgacttacc cttatcgcac tacaatggca ctcaacacct atcagtacag ggaaactaca
    47341 atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa
    47401 ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct acaagcgcag
    47461 ctgacgcgtc tcgatctggt aagccgcgaa gaattcgacg ttcagacgca agtcctgttg
    47521 cggactcgtg aaaaactggc gttactggag cagcgcatca gtgaactgga agcgcgcaat
    47581 aattcggtag cagatcttca atctcctccg gctatcccgc ctattgataa agcagaataa
    47641 agcaacacaa aaacgggcca gacggcccgt aatatcagtg ttctaattca agttctctgg
    47701 tttgcatcat ctgttggcaa taccaggcat aacgtgcgca aaaccaggtt ctatgttctt
    47761 catccatgtt accagtaacg gccagaatat cgaccgggcg accttctgcg tgcattctgg
    47821 caaaactacg cgccaggaaa tcgaaattat ttaaagaatt aagactatga tccataagca
    47881 catcctccgg ttatcgcata ccttttaagg ccatatgctt tttctgattt cagtattccc
    47941 caatcagtgt agtgccttta aacataacgt gcgtaaatat attgtccccg atcacacttt
    48001 ttagtgaaaa ataaaaaacc atattaaaag actttctctt ccagaacttc tctaacatgt
    48061 gttattatta acaaagacaa atgtaagcaa ggaaactatc ccaaagtaaa tattgcgtca
    48121 ctaaatggac attggagtga catatgattt tattcgccga ctataacacc ccttatcttt
    48181 tcgctatttc ctttgtactg ttgatcggcc ttttagaaat ttttgcgctt atctgcggtc
    48241 acatgctctc cggtgctctt gatgcacatc ttgaccatta tgattccata accacaggtc
    48301 atattagcca ggcacttcat tatctcaata tcggaagatt gccagctctc gtcgttctct
    48361 gcttgctggc tggttttttt ggtcttattg gcatcctgtt gcagcatgcc tgcatcatgg
    48421 tctggcaatc accactgtcg aacctgttcg tggttcctgt cagtttactg tttacgatta
    48481 ttgcggtgca ttataccggt aaaatagttg cgccctggat tccacgcgac catagttcag
    48541 ctattacaga agaagaatat attggtagca tggcattaat taccggtcat caagccactt
    48601 caggtaatcc ctgcgaagga aaactcaccg atcaatttgg ccaaatacat tatttactac
    48661 tggagcctga agaaggaaaa attttcacta aaggagacaa agtgttaatt atttgccgac
    48721 tttctgcaac gcgatatttg gcggaaaaca atccctggcc ccaaatattg taatttatta
    48781 atcaaaagga aatttaataa tggatgatat tgttaattct gtgccctcct ggatgtttac
    48841 cgcgattatt gccgtatgca ttctgtttat tattggaatt attttcgcca ggctctatcg
    48901 tcgcgcttcg gcagagcaag cttttgttcg tactggttta ggtgggcaaa aagtggtaat
    48961 gagcggtggc gcaatcgtga tgccgatctt tcatgaaata atccccatca atatgaatac
    49021 tctgaagctg gaagtcagcc gctcaaccat tgatagcctg attacgaaag atcgtatgcg
    49081 cgtcgatgta gtagtcgctt tctttgtgcg ggtaaaacct tcagtagaag ggattgccac
    49141 cgctgcccag acgctggggc aacgcaccct gtcgcctgaa gacttacgta tgttggttga
    49201 agataaattt gtcgatgccc tccgtgcaac agctgcgcaa atgaccatgc atgagttaca
    49261 ggatacccgc gagaacttcg tgcagggagt acaaaatacc gtagctgaag atctgtcgaa
    49321 aaacggcctg gaactggaaa gcgtttcact taccaacttt aaccagacct cgaaagaaca
    49381 tttcaatccg aacaatgcct ttgacgccga aggtttaacc aaactgactc aggagacgga
    49441 gcgccgtcgc cgcgagcgta acgaagttga acaggatgta gaagttgcgg tgcgtgagaa
    49501 aaaccgtgat gcactttcgc gcaagttgga gattgaacaa caagaagcgt ttatgacgct
    49561 tgagcaggag cagcaggtta aaacccgtac cgctgagcag aatgcgaaaa ttgcggcttt
    49621 tgaagctgaa cgtcgtcgtg aagcagagca gacgcgaatt ctggctgaac gacagattca
    49681 ggaaacagaa atcgaccgcg aacaggccgt ccgctcaaga aaagttgaag ctgaacgtga
    49741 agttcgcatt aaagagatcg aacagcagca ggtcaccgaa atcgctaacc agacgaaatc
    49801 gatcgctatt gccgccaaat cggaacaaca gtcccaggca gaagcgcgtg ctaaccttgc
    49861 actcgcagaa gcagtaagcg cccaacaaaa cgttgaaacc actcgccaga ccgcagaagc
    49921 cgatcgtgct aaacaagttg ccctaatcgc tgccgcgcag gatgcagaaa ccaaagcggt
    49981 tgaactgacc gtgcgggcaa aagcagaaaa agaagccgca gagatgcagg cggcggctat
    50041 cgttgagtta gccgaagcta cacgtaaaaa gggtctggcg gaagcagaag cacaacgtgc
    50101 gctgaacgat gctatcaacg tactttctga tgaacaaacc agccttaaat tcaaactggc
    50161 cttgttgcag gcgctgcctg cggtaataga aaaatccgtt gagccgatga aatccattga
    50221 cggtatcaag attattcagg tcgatggtct gaatcgtggc agcgctgcgg gtgatgcaaa
    50281 cacgggtaat gtggggggcg gaaacctggc ggaacaagcg ttatcagccg cactctctta
    50341 ccgcacacag gcaccgctga ttgactcctt gctcaatgaa attggcgttt caggcggctc
    50401 actggcggca ttgacttcac ccttaacctc aacaactccc gtcgcagaaa acgtagaata
    50461 aattttcaac tatgaacatc cctggcccaa aaagccgggg attttttata tctgcgttcc
    50521 gctaaaaact gcaaatgatc aggttgttgc aacgttttgc gcgaccgctc ggggaaggca
    50581 aaactgccac agggaaagca ttgcgcgagg tccggcgctc atcaacaatc gggaagtcag
    50641 caaggggctg aaacgggaaa gcccctcccg aagaaggggc cttacagaag gaaagggtta
    50701 tgatgaagct cgtcatcata ctgattgtgt tgttactcgt aagtttcgca gcttattaac
    50761 agccaatcag aggggggaga aatcctccct cacccttgtt cctttacttt aggttgaaaa
    50821 aaaatcagcg tcaagaaggt ttcattaaca catatccctg gcccaaaaag ccggggattt
    50881 tttatatctg cgttccgcta aaaggtgcaa atgctcagga cgttgcagcg ttttgcgtga
    50941 ccgctcgggg aaggcaaaac tgcttcaggg aaagcgttgc gcggggtccg gcgctcatca
    51001 acaatcgggg agacagcaag gggctgaaac gggaaagccc ctcccgagga aggggcctta
    51061 tagaaggaaa gggttatgat gaagctcgtc atcatactgg ttgtgttgtt actgttaagt
    51121 ttcccgactt actaacaact catcagaggg gggagaaatc ctcccttacc cttgttcctt
    51181 tactctaggt tgaaaaaaca acagcgtcaa taggcctgcc atgtacgaag cgagatctgt
    51241 gaaccgcttt ccggttagcc ttttttatcc tgttggatct tcttgataat gttagtggtc
    51301 gagcaaccgt cttcaaagtt gagcaccaac acttcgccgc cgttggccca gacttcttta
    51361 ctcccggcga tctcttccgg tttgtagtca ccgcctttca ccagcaggtc tggcaggatc
    51421 ccggcgatca gacgctgcgg cgtgtcttct tcaaacgaca ctacccagtc gaccgcttcc
    51481 agtgcgccca gcacaatcat acgctgttcg agtgggttta ccgggcggga atcccctttc
    51541 agccgtttgg tggaggcatc gctgttgacg gcaacaatca agcggtcacc cagcttgcgg
    51601 gcatttgcca gataagagac gtgcccggca tgtaggatat cgaagacacc gttggtcatt
    51661 accacttttt caccacgttt acgcgctgcc gctacagcca gcttcagttc ctcttcggtc
    51721 atcacgccaa agccggtatc tgcgcgtcca cgtacagcgt tttccagctc gatcggcgaa
    51781 accgtggagg ttcccagttt gccgaccacc acgccagccg ccgcattggc aaagaagcag
    51841 gcttcttcca gcgaattacc cgctgccagc gttgccgcca ggacgccaat caccgtgtcg
    51901 cccgcaccgg taacgtcata cacttcctgc gcttgggttg gcatatgcag cggcgcttta
    51961 cccggttgca gcagcgacat accctgttcg gaacgggtca ctaacagagc cgagagttcg
    52021 taatcggcaa tcagtttcat gccgcgctca acaatctctt cttcggtctt acatttaccg
    52081 acaacagctt caaattccga gagattcggc gttaacagcg tagcgccgcg gtagcgctca
    52141 aaatcggtac cttttggatc aatcagcacc ggaacacccg ctttacgcgc ctgttggatc
    52201 atctgctgta cgcttgccag cgcacctttg gcgtagtcag aaagcaccag cgcgccaatc
    52261 gaactcagcg cctgattaat ccgttcgtgc agcggttgcg gatcaacacc ttcgaaacct
    52321 tcttcaaaat ccaggcggat cagctgttgg ttgcgggaaa gtacccgtaa tttggtgatg
    52381 gtcggatgcg tcggtacaga aacgaagtcg catttgacgt tgacgtcggc cagagattta
    52441 ctcagcgcgc gcgctgcatc gtcaatgccc gtcaacccga ccaggcgtgc attagcaccg
    52501 agagaagcga tattcatcgc cacgttagcc gcgccgcccg gacgttcttc gatggtattc
    52561 actttaacca cgggcaccgg cgcttccggc gagatacgac tggtggggcc gtaccagtaa
    52621 cgatccagca tcacatcacc aaccaccatc actcctgcac gttcaaactc tggcagcgtt
    52681 actttcattc ctgtctcctg agagattcaa aatttgcgcg cgataatacc atacttcatt
    52741 cttccaccag ccacttctgc cagcttgccc gcaccaaatc acgctctgcg gtgaagcaat
    52801 cctccgacac atggcccggc aattcctgta atgccagatg atgaagttca tcgcgcagcg
    52861 tcgtgtaagc acgggtcagc gccatcgctt cctgctcttc cataatgtcg ttttgcgcca
    52921 gcaattccag aatgcgcacg ttgtctgacc agcgcgttaa cttcggtttt tcatgagcgt
    52981 agcgcaacac cagatattga gtaataaatt cgatatcggt aattccgcct tcatcggctt
    53041 tgatatcaaa gcgatcgcga tgtttattgc cgagatgagc gcgcattttt tcgcgcattt
    53101 cccgcacttc cgtttgcaga gttttacctt cacgcggcag cgtcataatc tcgcggcgca
    53161 ccgcgtcaaa gtgcgcggtg agctgcggat cgccgtacac tacacgcgca cgcaccagcg
    53221 cctgatgttc ccacgtccag gcctcgtttt tctgataatc agcaaatgct tctgcggatg
    53281 tcaccagcat tcccgccgcc ccggacgggc gtagccgtgc atccacttca tacaaaatgc
    53341 cggaagaagt acgcgtgctg aacagatgca taatgcgttg cgccagacgc agataaaact
    53401 gccgcccgtc gatttcccgc tcgccgtccg tcattacatc catcgggcaa tcatggagaa
    53461 agatcaggtc aagatcagaa ctgtagccta actcccagcc gcccagcttg ccgtagccga
    53521 ccaccgcaaa gccgcgacct tcacgttcat tcaggtgagt cggttttccg taacgggcaa
    53581 ccatttgaac ccacgcttgc tggaccaccg catctatcat tgcctccgcc agccaggtta
    53641 agtgatcgct cactttcatt accggcagcg taccggcgat atccgctgcc gcaatgcgta
    53701 acagttgcgc ctgtttgaac tgacgcagcg cctcaagctg ttgttcttcg tcatcttccg
    53761 gcacgcgcag caaatactgg cgcaactcat cacggtaagc atcggtcgcc gtcggctggt
    53821 agagagtgtt tggatcgagc aattcatcca gcaataatgg ataacgcgcc agctggctgg
    53881 caatcatcgg cgacgcggca cacagagaaa tcaaatgttt aagcgccgcg gggaattcac
    53941 tgagcaattc taaataggtg gtgcgggtaa caatccccac cagcaaggcg gtaatgcgcg
    54001 acagcgtgac ggcagcgtct tcacgcgcac agacatcact tagcagatgt ggcatcagat
    54061 ggtcgagcac ctgacgtcct cgcggcccga tggtgcgctt atccagctct ttgcggaaat
    54121 cagcaatcag cgttagcacc tgtttgcgat cattttcgct aagatgcgcc agcactggcg
    54181 tggtgtcatc ttcctgcaac gcatcctgcc acagctcacg ccactgttcc gacagcgact
    54241 cttcctgagt ttcactttca tcgtcgccaa tcaattcatt aaacacccgg cgcacattgg
    54301 tcatatgtgc ggtcagcgcc acggtcagtt gcggccagtc agcaaagtcc atcgcccacg
    54361 ccagccgcgc acggttaagc tcatcagaag gaagcgtctg ggtttgttcg tcgttaatgc
    54421 tttgcagcag gttttccaga cgccgcagga acagatacgc cactcgcaat tgttcagcat
    54481 cgttttcaga aagcagatgc agcgcggcaa tgacgctgag cgttggcagt aaagagcgcg
    54541 actgcagcga cggttcgcgc ccaccgcgaa tgagctggaa cacctgaacg ataaattcaa
    54601 tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt caaaccacgt cgacgcactt
    54661 cacgggcaat catccctttc atgttgcgca gcgactgaat cacgctgaaa tcgatgtaac
    54721 gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt agcatagacg ccttcgctat
    54781 cgcccataat ccgcgccttg accatcgcgt aacgctccca gtcgcgcccc tgctcctggt
    54841 aataatcttc cagcgccgca aagctcagca ccagcgggcc actttcgcca aacggacgca
    54901 gccgcatatc cacgcgatag acgaagccat cctgcgttgg ttgatccagc actttaatca
    54961 gccgctgccc catgcgggta aaaaactggg cgttatccag ttcccggcgt ccaccctgcg
    55021 tacaaccatg ttccggccag gcaaaaatca gatcgatatc agaggagaaa ttcagttccc
    55081 cgccgcccag cttacccatg cctaaaatca gcagcggttg cgcttcgccc tgcgcattgc
    55141 acggcgttcc ccactcgcgg cagcaggcgt catatagcca gtcacgcgcc gcaacaatca
    55201 gcgtctccgc cagatagctg agctgctgca atatgctctc ttcagtaacc agtgccagcg
    55261 tttgcgccca ggcgatacgt accatgatgc gccgacggaa cagccgcagc tcgcgcatta
    55321 acccggcttc gtcactcaca ttactgagcg cctcctgcaa ccatgcaacg taatgctgcc
    55381 attcgtcggc ctgcggcggt tggctttcca gttccgtcag ccactctgga tgcgcaatca
    55441 cgctgtcctg cacaaaatca ctgaaagtaa gtactgactt cgcctgtgcg ctaagtgatt
    55501 cctcggctaa aggctctggc agccgctcaa caacggtctg ccagtactgc tgtaacggtg
    55561 aagagagcgg cttcataagc gattttatcc ttgcctgatt aacgttttcc gctgtgcaac
    55621 cagaacggtt cctgattgtt tgcctcatta cggaaatgtt caatttcgat gcgttgcccg
    55681 gtcgcaatag cgtgatgcag cccctgccag ttctccagcc aggcttgcgc gacgacagga
    55741 tcatagtaac ccgccagcaa cagtattgag tcaatatcac gcgtcaggcg tggcaactgg
    55801 tcacggtagc gatcgcctaa cggctggcag aaaacgcttt tcagttcagc ggcatggcgg
    55861 gaaagatgga tatcggcaaa gcgtttgaag gagtcgctga ttttgccctg cgctttggca
    55921 tctaaaaatg gctgccatgc tttgcttacc aaccattcgg tcaacgccag cttcgccatt
    55981 gccgtttcgg tagagtagac cgccgtcacg gcagaaaccg cagaagcaat ggtcgcttcg
    56041 cattgggtca gcaaatcacg taagtgagtg ctcgctttac gcggcacaat accaccgaac
    56101 agcatcagcg tatgacggac caggctaatg gctgccagca cctgttcttt cgccgcatcg
    56161 ttgccgcgta cccacagttc ttcatgatac tgccattgcg ctaacgccag ctcgagcgcc
    56221 gcttccagcc cctgttccac atcggctttt gccgcaacat gcaaaatggt ggtcggtttg
    56281 atttcacgcg ccggattgcc ctgcgccaga tgatagccac gcgccgcttt gctcaggctg
    56341 ccctggcgta atccggtttg cgataccagt tggttcgcca gtttcagcac cgcgcgcgtg
    56401 tcgccgctaa gcagttccag ttccagctca cagataggtt cagcaaattc ctccgctttc
    56461 acctccccct gatcgagagc gatttcaatt cggctgccat cgaccgccac cagccatttt
    56521 tcgcgataaa aatcggtgct gaacagcggt tgtacgcggg aagcgagatc ggcaggcaat
    56581 tcgccgttcg gccagacttc cgagggaaac tgcgccaggt cgagcgtcgg ttcgctcaac
    56641 gccacgttat attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc aactttcatg
    56701 gtcatctcat agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg cccacgcagc
    56761 cagttatccg gcgtttcgta gtaaatattc agcaactgca cggggtcatg gtgctcgccg
    56821 cccagcgtat tgagatggtc acgcaacgcc tcaacggcac tgtgattaac aataaacttt
    56881 aattcgattt cctgagccat ggcctttact tatcggttat gtcacataag tgacgatgaa
    56941 cggcgaactt aatgcgatct tttttgtcag tagatagtat tttgcgccaa attgccatgc
    57001 aacgggcaat ttgacgggcg taaaagtttg aagcagtggc aagacctgac acagaggatt
    57061 ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc actactatcg ttccactttc
    57121 catgacaata acgacagcct gatgccaaaa ttacgcctga tcggattaac tttactcgca
    57181 cttagcgcga ctgccgtctc acacgctgaa gaaacgcgct atgtttctga cgaactgaat
    57241 acctgggtcc gtagcggtcc gggagatcat tatcgcctcg tgggcacggt taacgccggc
    57301 gaggaagtga ccttattaca aactgacgcc aacaccaatt atgcccaggt gaaagacagc
    57361 tctggccgta ccgcctggat cccgttgaaa caacttagca ctgagccaag cctgcgctcc
    57421 cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg ataaactcac caatatcgat
    57481 aacacctgga atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag cgacagcgtg
    57541 atcaacgggt taaaagaaga aaatcagaaa ctgaaaaacg agctgattgt cgcgcagaaa
    57601 aaggtcgatg ccgccagcgt acagctggat gacaaacagc gcaccatcat catgcaatgg
    57661 tttatgtatg gcggtggcgt gctggggctt ggcttgctgc tcggtctggt actgccgcac
    57721 ctgatcccaa gccgcaaacg caaagatcgc tggatgaact aaatcgcctt cttcgttgca
    57781 ctgtcttatt attaggaatt gtgtatttaa cgaagggatg atggcgtgaa gatttatctg
    57841 gtcggtggtg ctgttcggga tgcattgtta gggctaccgg tcaaagacag agattgggtg
    57901 gtggtcggca gtacgccaca ggagatgctc gacgcgggct accagcaggt aggccgcgat
    57961 tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg cgctggcacg taccgaacgg
    58021 aaatccggtt ccggttacac cggttttacc tgctatgccg caccggatgt cacgctggaa
    58081 gatgatctta agcgtcgcga tctgaccatt aatgcgctgg cccaggacga taacggcgag
    58141 attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg ccatgtttcc
    58201 cccgcttttg gcgaagatcc gttacgcgta ctgcgcgtgg cgcgttttgc tgcgcgttat
    58261 gcccacctcg gttttcgtat tgccgatgaa actctggcgt tgatgcgcga gatgacccat
    58321 gcgggtgaac tggaacacct gacgcctgaa cgggtgtgga aagagacgga aagcgccctc
    58381 accacccgca atccccaggt gttcttccag gtactgcgcg attgcggtgc acttcgcgtt
    58441 ttattcccgg aaattgacgc cctgtttggc gttccggccc ctgccaggtg gcatccggaa
    58501 atcgacacgg gtattcatac cttaatgacg ctctcaatgg cggcgatgct gagtccgcag
    58561 gtcgatgtcc gtttcgcaac tttatgccac gatctcggta aaggactgac gccgccagaa
    58621 ctctggccgc gtcatcatgg tcatggcccg gcgggtgtta agttagtgga acaactatgc
    58681 cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca gactggtggc tgagtttcac
    58741 gatctcatcc acaccttccc aatgctgaac ccaaaaacca tcgtcaaatt atttgattcc
    58801 atcgatgcct ggcgtaaacc gcagcgtgtc gagcaactgg cgctgaccag cgaggctgac
    58861 gtgcgcggca gaaccggttt tgaatcagcg gactacccgc aaggccgttg gttgcgcgaa
    58921 gcctgggaag tggcgcagtc agtgccgaca aaagccgtcg ttgaagcggg atttaaaggc
    58981 gtggagattc gcgaggagct gacccgacgg cggattgcgg cggtagccag ctggaaggaa
    59041 caacgttgcc caaagcctga atgagtattg ggcattttgc cggatgcggc gtaaacgcct
    59101 tatccggcta catgtcaacg acagttgtag gcctgataag acgcgcaagc gtcgcatcag
    59161 gcgttgattg ccggatgcgg cgtgaacgcc ttattcggcc tacgcaatcg tggacaacgc
    59221 caggcatcca acactattcc tcaatcaaaa gaacacgaca tacaccgcag ccgccacaat
    59281 aaagcgataa atggcgaacg ggataaacga aatgcgctta atcaattgca ggaaggtttt
    59341 aatcgctatc agcgccacca caaaagcggt gataaaccca acggcaaaca tcgggatatc
    59401 gccggttgtc aggaagcccc agcttttgta gagatcgagc gccgttgcgc ccatcatcat
    59461 cggcaccgcc agcaggaacg aaaactcgga agcagcgtaa cggctcaccc ccatcagcat
    59521 cccacctgaa atggtcgccc cggaacggga gaaacccggc cacagcgcca gacactggaa
    59581 acagccaatc ataaatgcct gacgataggt catatcatca agacccggcg cacgcggctc
    59641 tttcggcttc aggcattcgg cggcaatcag caacaaaccg ccaacgacca gcgcatacat
    59701 cacatttatc gggttaaaca atgacttaat cgtgtcgtgg aacaacagcc ccagtaccac
    59761 cgccggaatc atccccagca aaatgtggat cagcgttaaa cgacctttgc tttctccttc
    59821 gtgctgcaac gggcggccaa agtggatgcc aatcaggcca aacagacgcc gccagaacat
    59881 cactactacc gccagaattg atcctaactg gatcacaact tcaaaggttt tcgccgtgtc
    59941 gccctcaaac cccaacaagt gaccgacaat aatcatatgg cccgtgctgg ataccggcag
    60001 aaattctgtc aatccttcga ccacacccaa tattgccgct atcagcagcg agtgcatatc
    60061 gctcatcaat aaacccctaa attattaaaa tgtaccgctt gtccgaacta ctgcagtatg
    60121 accaggttat aaccgtttgg tttaacagct gtaaaattaa ttattttctt tcagattatt
    60181 gccacgctca atgattacgc caacattcgc cgcccgcgcc actgcgcctg gcttgctgag
    60241 tttgatacgc acccacggcg agttgaagcg tgctaacagc agctccgcca cctcttccgc
    60301 cacgcgctcc accagcgcaa aacgtgcccc ctcgacgtgg ctgaccaccg tttctgcaat
    60361 gtcagcgtag ctgaggcaat ccgccacatc atcacttttc gccgctttac ggttatccca
    60421 cgccatttcg atatcgaaca ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc
    60481 aatagtggtg attaccgaaa gttgctctat aaatacaata tccatcacgt cctgcctgct
    60541 ttttggctaa ccggatacca cttccggcga aatgtgcgta ttatccacag attcatcgtt
    60601 gaacacgaat tttcaaaacg gaacagctta tgagtgcaat cgcgcctgga atgatcctca
    60661 tcgcgtacct ctgcggctcc atttccagtg ccattctggt ttgccgcttg tgtgggctgc
    60721 ccgatccgcg aaccagcggc tccggcaatc caggcgcaac caatgtgtta cgtatcggtg
    60781 gcaagggagc agccgtagca gtactgattt tcgacgttct gaaaggaatg ttgcccgtct
    60841 ggggcgcgta tgaattaggt gtcagcccct tctggctagg cttaattgcc atcgccgcct
    60901 gtcttggaca catctggccc gttttcttcg gatttaaagg aggaaaaggc gttgctaccg
    60961 cttttggtgc catcgcaccc attggctggg atctcaccgg agtaatggcg ggaacctggt
    61021 tactgaccgt gctattgagc ggatactcgt cgctgggagc gattgtcagt gcactgattg
    61081 ctccgtttta tgtctggtgg tttaagccac aattcacctt cccggtttcg atgctctctt
    61141 gcctgatcct gctgcgtcat catgacaaca tccagcgtct gtggcgtcgt caggagacaa
    61201 aaatctggac gaaattcaaa agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc
    61261 tacatgacct gatagccttc atcgggtttg cccagccgtt gctggcacca tgccgccaga
    61321 aattccacgc agacacgtaa tttcatgctg cgatagagcg gctcccggta aacagcccag
    61381 atattggcgc tttgtgcata ctccggcaat acttgcacca gtttgccact ctccagaaac
    61441 ggcaacacat cccactcgga acgcagcata atccctttgc cctccagcgc ccattgcagc
    61501 acaatctcgc cgctattgga ggaaagatgc ccgctaacct tcaccgattt tttctcctga
    61561 ccgttcccca actcccatat tccgtgggtc atatcgcgct ctttggtcac cagacagtca
    61621 tgacgactta attcctgtag ggattgtggc tgcggatatt tttgcagata ctcaggtgct
    61681 gcgcataata ttcttttatt ttttgtcaaa agatgcgcaa tataataatc aggaatttcg
    61741 tcattaatac gaatatccag atcaatatta tcctgcacta aatcaatttg tcgatcgaac
    61801 aattcaaaat gcacctgtaa ctcaggataa ttgcgcatca gttcggtaat ggctggcgca
    61861 atatggctgc gcccaaaacc aaaactgcaa ccaatacgaa tcatcccctc cgggcgcgtt
    61921 ttgatttgcg tgacgtcatc gaccagtcgc tgatattggg tgaggatttc cagcgcatgt
    61981 tcgtaacaac gttgaccact ttcagtcagc gccacgccgc gagcggagcg gttcagcagc
    62041 gttgttgcca acgtattttc aaggatttgg atccgcttgg tgacaaatgc gggcgtctgc
    62101 ccaagcgtcg ccgctgcggc gctaaaactc ccgctgtgga cgatttctac cagtacctgc
    62161 aaatctttgg ctaaaggcca gctattcagc atcgttaatt atccgcagtt gtgataagcg
    62221 cagtgtattt cgcaaaacat tgccattccc tgctctggcg caatattcat acttattttc
    62281 gcgttgattc acgaactgtt aattacatat tcacggtggc aaaaaatata aaaccacatt
    62341 tttagtggta gtttgtggcg gtgaattttt ccagacaaat acagaaactg gagttgccat
    62401 gatgagcgaa agtaataagc aacaggcagt gaataagttg acggagattg tcgctaactt
    62461 taccgccatg atttctaccc gaatgcctga tgacgtggtg gataaactaa aacagctaaa
    62521 ggatgccgaa acgtcgtcga tggggaaaat tatctaccat acgatgttcg acaacatgca
    62581 aaaagcgatt gacctgaatc gtcctgcctg tcaggacacc ggggagatta tgttcttcgt
    62641 taaagtcggt tcccgcttcc cactgcttgg cgagctgcaa agcatactca aacaagccgt
    62701 ggaagaggca accgtcaaag cgccactacg tcacaatgcg gtagaaattt ttgacgaagt
    62761 gaacacaggc aaaaataccg gcagcggcgt accgtgggtc acctgggata tcgtccccga
    62821 cggtgacgat gcggaaatcg aagtttacat ggcaggcggc ggctgcacgc taccaggccg
    62881 ctcgaaagtg ttaatgccgt cagaaggcta cgaaggcgtg gtgaaattcg tcttcgaaaa
    62941 tatctccacc ctcgcagtaa acgcctgtcc accggtactg gtgggcgtgg gcatcgccac
    63001 ctcggtggaa accgccgccg tgctctcgcg aaaagccatt ttgcgcccga ttggcagccg
    63061 ccaccccaat ccaaaagcgg cagagctgga gctacgcctg gaagaaggac tcaaccgtct
    63121 ggggattggt ccacaagggc tgactggcaa cagttcagtg atgggcgtgc atatcgaatc
    63181 tgccgctcgc catccgtcaa ccatcggcgt tgctgtttct acaggctgct gggcgcatcg
    63241 tcgcggcaca ctgctggttc atgccgatct ctcctttgaa aatctgtctc acacccggag
    63301 cgcgttatga aaaagatcct gacaaccccg atcaaagctg aagatctgca agatattcgc
    63361 gtcggcgatg tgatctacct gaccggtacg ctggtgacct gccgcgacgt ttgtcaccgc
    63421 cgcttgatcg aactgaaacg tccgatccct tacgatctca acggcaaagc gattttccac
    63481 gctggcccca tcgtgcgcaa aaacggcgac aaatgggaga tggtctccgt cggtccgaca
    63541 accagtatgc gtatggaaag ttttgaacgt gaatttatcg agcagaccgg cgtgaaactg
    63601 gtggtcggca aaggcggaat ggggccgctg accgaagaag gctgccagaa attcaaggcg
    63661 ctacatgtga ttttcccggc aggctgcgcg gtgctggcgg caacccaggt ggaagagatt
    63721 gaagaagtgc actggacaga actcgggatg ccggagtcac tgtgggtctg ccgggtcaaa
    63781 gagttcggcc cgctgattgt ctctattgat acccacggca acaacctgat agccgaaaac
    63841 aaaaagctgt tcgccgaacg ccgcgatccc atcgtggaag agatctgcga gcacgtccat
    63901 tacatcaaat aaccctcccg gagaggctcg ccccctctcc tttttcgcag gcataacacg
    63961 atgaaacctt ccactgaatg gtggcgatac cttgcgccgc tggcggtcat cgccattatt
    64021 gctctaattc cggttcccgc agggctggag agtcatacct ggctctactt tgccgttttt
    64081 actggcgtaa tcgttggact gatcctcgaa cccgtgccgg gtgccgtggt ggcgatggtg
    64141 ggtatatcca tcatcgccat actctctccc tggctgctgt tcagccccga acagctcgct
    64201 cagccaggct ttaaattcac tgcaaaatcc ctctcgtggg ccgtttccgg tttttctaat
    64261 tcggttatct ggctgatttt cgccgccttt atgtttggca caggctatga aaaaaccggg
    64321 ctggggcgac gtatcgcgct gattctggtg aaaaagatgg ggcatcgcac gctgtttctt
    64381 ggctatgcgg tgatgttctc cgagctgatc ctggcacctg tcacaccgtc caactcggcg
    64441 cgtggtgcgg ggattatcta tcccatcatc cgtaacctgc cgccgctcta tcaatcacaa
    64501 ccaaacgaca gcagttcgcg cagtattggc tcgtacatca tgtggatggg gattgttgcc
    64561 gactgcgtga ccagcgccat tttcctgacg gcgatggctc ctaacttgct gttaattgga
    64621 ctgatgaaaa gcgcatctca cgccacgctg agttggggcg actggttcct cgggatgttg
    64681 ccgctcagta ttttactggt cctgctggtt ccctggctgg cttacgtgct gtacccgccg
    64741 gtactgaagt ctggtgatca ggtaccgcgc tgggcagaga cggaactgca ggcaatgggc
    64801 ccgctctgtt cgcgtgaaaa acggatgctg gggctgatgg taggcgcgct ggtgctgtgg
    64861 attttcggcg gtgattatat cgatgccgcg atggtcggtt acagcgtggt ggcgctgatg
    64921 ctgcttctgc gcattatcag ttgggacgat attgtcagta ataaagcggc gtggaacgtt
    64981 ttcttctggc tggcctcgct tatcaccctc gcgaccggac tcaacaacac cggttttatt
    65041 agctggtttg gcaaactgtt agcaggcagc ttaagcggtt attcgccaac gatggtgatg
    65101 gtggcgttga ttgtggtgtt ttatctactg cgctactttt tcgccagcgc cacggcgtat
    65161 acctccgctc tcgcaccgat gatgatcgcc gccgcgctgg cgatgccgga aatcccgctg
    65221 ccggtgttct gcctgatggt tggcgcggca attggtctgg ggagcattct tacaccatac
    65281 gccaccggac ccagtccgat ttactacggt agtggttatc tgccaacggc ggattactgg
    65341 cgactggggg cgatttttgg gctgatattc ctcgtgttgc tgatgattac cggcttactg
    65401 tggatgcccg tggtgttgct ttaagtgagg acggtgccgg aaattgctct ccggcaccca
    65461 atagttttac gcagccggta actccgccag cggccagcgc ggacgcacgc taacaccgag
    65521 atccgccgtc gcgcctgctt taaaccgcac cattccggca taggcgatca tcgcaccgtt
    65581 atcagtacaa aattccggac gcgcgtagaa cacttcgccg cggcgttttt tcatcatttc
    65641 cgccagtttc gcccgcagcg tgcggttagc actcacgccg cccgccatga ccagtcgctt
    65701 aaagcccgtc tgatccagtg ctcgcttaca cttaatcatc aacgtatcaa ccaccgcatc
    65761 ttcaaaggcg cgggcgatat cagcacgcgt ctggtcgtcg gtgccgttgt cacgaatcgt
    65821 gttcgccgca aaggttttca gaccagaaaa gctgaaatcc agccccggac ggtcggtcat
    65881 cggacgcggg aaaacaaagc gcccggcagt accctgagcc gccattttcg acagtaacgg
    65941 tccgccagga taatccagcc ccagcagctt cgcggtttta tcaaacgctt caccggcggc
    66001 atcatcgata gactcgccga gcagctcgta ctgaccaatg ccagtcacgc tgattaactg
    66061 cgtatgaccg ccggaaacca gcagcgcaac aaacggaaat tccggcgggt tatcttccag
    66121 catcggcgct aacagatgcc cttccatatg gtgtacaggg atcgccggaa cgttccaggc
    66181 aaacgccaga gaacgcccca cggtcgcgcc aaccagcagt gcgccgacta atccagggcc
    66241 tgcggtatag gccacagcat caatgtcttt tgccgttaaa ccagactcct ttagcgccgc
    66301 ctggatcaac ggtacggttt tacgcacatg atcgcgggag gccagctcag gcacaacgcc
    66361 cccgtagtca gcgtgcaatt tcacctgact atacaattgg ttggctaaca aacctttttc
    66421 atcgtcgtaa atggcgatgc cggtttcatc gcaggaagtt tcaataccca gtacacgcat
    66481 gacttgtttt acctcgcttt attaccgcgc agtgtaggac caatgcgggt tgatgtaaaa
    66541 ctttgttcgc ccctggagaa agcctcgtgt atactcctca cccttataaa agtccctttc
    66601 aaaaaaggcc gcggtgcttt acaaagcagc agcaattgca gtaaaattcc gcaccatttt
    66661 gaaataagct ggcgttgatg ccagcggcaa accgaattaa tcaaaggtga gaggcacatg
    66721 ccggtaatta aagtacgtga aaacgagccg ttcgacgtag ctctgcgtcg cttcaagcgt
    66781 tcctgcgaaa aagcaggtgt tctggcggaa gttcgtcgtc gtgagttcta tgaaaaaccg
    66841 actaccgaac gtaagcgcgc taaagcttct gcagtgaaac gtcacgcgaa gaaactggct
    66901 cgcgaaaacg cacgccgcac tcgtctgtac taattccccg agagcgttgc tctccgatca
    66961 gaccgagttg tagttgtaag gccgtgcttc cgaaaggaat gcgcggctta ttttcgttta
    67021 tgaattgcta aaaatcgggg cctatggctg gacgaatccc acgcgtattc attaatgatc
    67081 tgctggcacg caccgacatc gtcgatctga tcgatgcccg tgtgaagctg aaaaagcagg
    67141 gcaagaattt ccacgcgtgt tgtccattcc acaacgagaa aaccccgtca ttcaccgtta
    67201 acggtgagaa acagttttac cactgctttg gatgtggcgc gcacggcaac gcgatcgact
    67261 tcctgatgaa ctacgacaag ctcgagttcg tcgaaacggt cgaagagctg gcagcaatgc
    67321 acaatcttga agtgccattt gaagcaggca gcggccccag ccagatcgag cgccatcagc
    67381 ggcaaacgct ttatcagttg atggacggtc tgaatacgtt ttaccaacaa tctttacagc
    67441 aacctgttgc cacgtctgcg cgccagtatc tggaaaaacg cggattaagc cacgaggtta
    67501 tcgctcgctt tgcgattggt tttgcgcccc ccggctggga caacgtcctg aagcggtttg
    67561 gcggcaatcc agaaaatcgc cagtcattga ttgatgcggg catgttggtc actaacgatc
    67621 agggacgcag ttacgatcgt ttccgcgagc gggtgatgtt ccccattcgc gataaacgcg
    67681 gtcgggtgat tggttttggc gggcgtgtgc tgggcaacga tacccccaaa tacctgaact
    67741 cgccggaaac agacattttc cataaaggcc gccagcttta cggtctttat gaagcgcagc
    67801 aggataacgc tgaacccaat cgtctgcttg tggtcgaagg ctatatggac gtggtggcgc
    67861 tggcgcaata cggcattaat tacgccgttg cgtcgttagg tacgtcaacc accgccgatc
    67921 acatacaact gttgttccgc gcgaccaaca atgtcatttg ctgttatgac ggcgaccgtg
    67981 caggccgtga tgccgcatgg cgagcgctgg aaacggcgct gccttacatg acagacggcc
    68041 gtcagctacg ctttatgttt ttgcctgatg gcgaagaccc tgacacgctg gtacgaaaag
    68101 aaggtaaaga agcgtttgaa gcgcggatgg agcaggcgat gccactctcc gcatttctgt
    68161 ttaacagcct gatgccgcaa gttgatctga gtacccctga cgggcgcgca cgtttgagta
    68221 cgctggcact gccattgata tcgcaagtgc cgggcgaaac gctgcgaata tatcttcgtc
    68281 aggaattagg caacaaatta ggcatacttg atgacagcca gcttgaacga ttaatgccaa
    68341 aagcggcaga gagcggcgtt tctcgccctg ttccgcagct aaaacgcacg accatgcgta
    68401 tacttatagg gttgctggtg caaaatccag aattagcgac gttggtcccg ccgcttgaga
    68461 atctggatga aaataagctc cctggacttg gcttattcag agaactggtc aacacttgtc
    68521 tctcccagcc aggtctgacc accgggcaac ttttagagca ctatcgtggt acaaataatg
    68581 ctgccaccct tgaaaaactg tcgatgtggg acgatatagc agataagaat attgctgagc
    68641 aaaccttcac cgactcactc aaccatatgt ttgattcgct gcttgaactg cgccaggaag
    68701 agttaatcgc tcgtgagcgc acgcatggtt taagcaacga agaacgcctg gagctctgga
    68761 cattaaacca ggagctggcg aaaaagtgat ttaacggctt aagtgccgaa gagcgatcgg
    68821 gaagcccccg acagccgcac tgagaggcag cggcaaatat ataagtacgc cctcgtaatt
    68881 atcgttggcg gtaaataacc gttggatttc agcgttaacg gctgaaggac atcgggtcaa
    68941 tcgcccaaca ccaacctcat gaaataagtg tggataccgt cttatggagc aaaacccgca
    69001 gtcacagctg aaacttcttg tcacccgtgg taaggagcaa ggctatctga cctatgccga
    69061 ggtcaatgac catctgccgg aagatatcgt cgattcagat cagatcgaag acatcatcca
    69121 aatgatcaac gacatgggca ttcaggtgat ggaagaagca ccggatgccg atgatctgat
    69181 gctggctgaa aacaccgcgg acgaagatgc tgccgaagcc gccgcgcagg tgctttccag
    69241 cgtggaatct gaaatcgggc gcacgactga cccggtacgc atgtacatgc gtgaaatggg
    69301 caccgttgaa ctgttgaccc gcgaaggcga aattgacatc gctaagcgta ttgaagacgg
    69361 gatcaaccag gttcaatgct ccgttgctga atatccggaa gcgatcacct atctgctgga
    69421 acagtacgat cgtgttgaag cagaagaagc gcgtctgtcc gatctgatca ccggctttgt
    69481 tgacccgaac gcagaagaag atctggcacc taccgccact cacgtcggtt ctgagctttc
    69541 ccaggaagat ctggacgatg acgaagatga agacgaagaa gatggcgatg acgacagcgc
    69601 cgatgatgac aacagcatcg acccggaact ggctcgcgaa aaatttgcgg aactgcgcgc
    69661 tcagtacgtt gtaacgcgtg acaccatcaa agcgaaaggt cgcagtcacg ctgctgctca
    69721 ggaagagatc ctgaaactgt ctgaagtatt caaacagttc cgcctggtgc cgaagcagtt
    69781 tgactacctg gtcaacagca tgcgcgtcat gatggaccgc gttcgtacgc aagaacgtct
    69841 gatcatgaag ctctgcgttg agcagtgcaa aatgccgaag aaaaacttca ttaccctgtt
    69901 taccggcaac gaaaccagcg atacctggtt caacgcggca attgcgatga acaagccgtg
    69961 gtcggaaaaa ctgcacgatg tctctgaaga agtgcatcgc gccctgcaga aactgcagca
    70021 gattgaagaa gaaaccggcc tgaccatcga gcaggttaaa gatatcaacc gtcgtatgtc
    70081 catcggtgaa gcgaaagccc gccgtgcgaa gaaagagatg gttgaagcga acttacgtct
    70141 ggttatttct atcgccaaga aatacaccaa ccgtggcttg cagttccttg acctgattca
    70201 ggaaggcaac atcggtctga tgaaagcggt tgataaattc gaataccgcc gtggttacaa
    70261 gttctccacc tacgcaacct ggtggatccg tcaggcgatc acccgttcta tcgcggatca
    70321 ggcgcgcacc atccgtattc cggtgcatat gattgagact atcaacaaac tcaaccgtat
    70381 ttctcgccag atgctgcaag agatgggccg tgaaccaacg ccggaagaac tggctgaacg
    70441 tatgctgatg ccggaagaca agatccgcaa agtgctgaag atcgccaaag agccaatctc
    70501 catggaaacg ccgatcggtg atgatgaaga ttcgcatctc ggggatttca ttgaggatac
    70561 caccctcgag ctgccgctgg attctgcgac caccgaaagc ctgcgtgcgg caacacacga
    70621 cgtgctggct ggcctgaccg cgcgtgaagc gaaagttctg cgtatgcgtt tcggtatcga
    70681 tatgaacacc gatcacacgc tggaagaagt gggtaaacag ttcgacgtta cccgcgaacg
    70741 tatccgtcag atcgaagcga aggcgctgcg caaactgcgt cacccgagcc gttctgaagt
    70801 gctgcgtagc ttcctggacg attaatcggt aggccggatc aggcgttacg ccgcacccgg
    70861 cacctgtccc tttgcacaaa cgccaccttt tcggtggcgt tttttatcgc ccacgcacca
    70921 ccagcgcctg gtccagctcg cgatacgctt caaccagttt ctccagtgaa acgcgactta
    70981 aaccactggg atttggcagc acccaaatct gcgtcgaacc aatgctgagc gtttgtttcc
    71041 cccactgtgc accgcgctgg ctgaatccct gttcatatgc ttgtttaccc agaatcgcca
    71101 acgcttgcgg ctgataatct tcaatttttt caatcagctt acgcccacct gcgtgtagct
    71161 cttgctttga aatttcattg gcttgcaccg ttggacggtc taccagtttg gtgacgccac
    71221 aacgataatc cagcagatgc tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg
    71281 cctgatatat caccttccag aagcgatttg ccggatgagc aaagggaaaa ccagtcccgg
    71341 cggatgaaag cccagggttg ataccgcaaa acacgacccg taaccctgga gccaaaatat
    71401 cctcaaccat atttactcct ttctaacatc atcaggaaaa gtataacgga ttgaaaatac
    71461 attgtttata aaaacagcag gcgcgcggta atggctggat tgcgacacgg agttacttta
    71521 taatccgcta ccatggcccc ttagctcagt ggttagagca ggcgactcat aatcgcttgg
    71581 tcgctggttc aagtccagca ggggccacca gatatagcaa aggctgacga gaaatcgtca
    71641 gcctttttct ttttatatat cagttacttt gcgtgccagt aagccgctgc acgtacccgc
    71701 tgtgggtcat actgttccgc ttcaaagcgg cggcttaaat tcttaacgac tttaccttcg
    71761 ccggttatcc agatgaagta atcatcggca gggatttgca tctgcgccag acgcgcatct
    71821 accgcctgct catcatgtgc cagccattcg atattaaaac catcaaggtg cgcgagataa
    71881 tcctgacagg cgttatcccg cacgctaacc agcgcactaa cttgcggttt aacggcaagt
    71941 ttgctcaacg tttccaggcg gcggcgcaat gcgggcattc cggattcatc gcagacatac
    72001 agctgatacg cgtaatcttc cggcaccacc agcgaaccgc gcggacctgc caccgtgagt
    72061 ttatcgcccg gttgggcctg cattgcccag ctactggcga cgccgtcttc gtgaataaag
    72121 aaatcaatcg ccagttcatg gcgtagttcg tcatacagtg gcgtatagtc acgcgacggt
    72181 gggcgtggtc cttccggcca gacgatgccc tcttccgtta ccgttggcgg cacaaagtga
    72241 gcgtcaggtt gaggaaagaa gagtttgctg tgatcgtcaa agccgcgcga tgtaaaaccg
    72301 tccagcgcct cgccgccaag gacaatgcgc tgaaaaccgg cgctgatacg ctcaacgcgt
    72361 agcacagtca gttcacggaa ccgcagatca ttgcgaacgc gctgcgggta gcggggggag
    72421 ttattcattg ttatcgcctt cgtgatggta atcagatata tctaaataat tttcgcaaat
    72481 gataatgatt gttaatcatg ataaatgcaa gcgatttgta gaactgatat atctatagtc
    72541 tgataagacg aaccgcctct tctcaggcat cattactcaa cgccggatgc ggcgtgaacg
    72601 ccttatccgg cctacgtgtg agatgagtcc acatttaaaa atcattttta ttcttgcaag
    72661 agtgctcata tcagatataa attagatata tctaattaag caaaaggagg ctgatatgag
    72721 ccatcatcac gaagggtgtt gtaaacatga aggccagcca cgccatgagg gctgctgcaa
    72781 aggtgagaag tcagaacacg agcactgcgg acacggtcac cagcatgaac acggtcaatg
    72841 ttgcggtggt cgccacggtc gcggcggcgg tcgtcggcaa cgtttctttg gtcacggtga
    72901 attacgtctg gtgattctgg atattctctc gcgcgatgac agccacggtt acgaattgat
    72961 taaagcgatt gagaatctaa cccaggggaa ttacacccca agcccgggcg tcatctaccc
    73021 aacgctggat tttctgcagg agcagtcgct gattaccatc cgcgaagagg aaggtggtaa
    73081 gaagcagatt gcactgaccg aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt
    73141 ggagatgatt gaagaacgca tcaaagcgcg ttgcgttggt gctgcgctgc gccagaaccc
    73201 gcaaatgaag cgggcgctgg ataattttaa agcggtgctg gatttacgcg tcaaccagag
    73261 tgatatcact gatgcacaaa taaaaaagat cattgcggtg atcgaccgcg ccgcttttga
    73321 tattacgcaa ctggattaat caccgattgc cggatgcggc gtaccctaca taccccgtag
    73381 gcctgataag atgcgccagc atcgcatcag gcattgtgct ccaaccgccg aatcctgcat
    73441 accgattaat gcagcaccgt caccgcgtct tccagtcggc tggcgcggtg tttcaccatc
    73501 tccgacacct gcgcactctc ttccaccagc tcggcatttt tctgggtgat gaggttaagc
    73561 tcatccactg cacgggtcag gctggaaaga ccatcggcct gttccagcgt tgaatggcta
    73621 atctgggcaa tcaactgggt gacgtttttc acctgtgcca caatatcttc catcgtacgt
    73681 ccggcggcgt gtacctgctg cgaaccggat tgcaccttat cagcactggc atcaatcagc
    73741 ttgcgaatat cgttggcagc attggcgctg cggctggcta aatgacgcac ttcccccgcc
    73801 accactgcaa aacctttgcc ctgttcgccg gctcgcgccg cttccaccgc cgcattcagg
    73861 gccagaatat tggtctgaaa cgcaatatcg ttaatcagcg aagtaatggt gccaatgcgc
    73921 tgggtactgt cggcgatatc gtccatagtc ttgatcaccg tggtcatcgc ctccccgccc
    73981 tgcactgccg cattactggc agtgattgac agtttatcgg cagccgacgc cgtggcagag
    74041 ttctgtttca ccgacgccgc catttggttc atggtcgcca ccgtttgctg aacgttatca
    74101 actgtctgct gggtatgttc gttcagttca tcggtgcctt tcgccagcgt ctcactgcca
    74161 tttctgacac tggacacctg gcttgagaca tcgttaatta gccaacggca catcaggcca
    74221 agctgcccta ccgcgcgtaa tgtcagcccc agctcatcgc tgcgattcag atgctcaaca
    74281 ctattacgct ctccggtcgc caccttcagt gcctgacggg cgacattttc tatcgggcgg
    74341 acaatctgcc attcaaaaca ggcgcttgcc aacaatacca ctaacgcaca gaggatatac
    74401 gtcaccaccg gggcagcaac aaaccaaagc atggccgcca gcaagataaa catcagggtc
    74461 atcactccac gcgcccgcca gcgaagcggt aatgaaggca gtttacccag ccagccttta
    74521 cgcaccacca ggcctttatg aatacgctta ccggtacgtc cggcgttcag cgctttgtac
    74581 agcggctcca ccgccgcgat ctcttcatcc gtcgcccggg tacgaatcga catatagcca
    74641 ctgatttttc cctcgcgcac catcggtacc gcattggccc gcacccaata atggtcacca
    74701 tttttgcggc gatttttcac gatgccgctc cagggctccc cttttttcag ggtgaaccac
    74761 atatctgcaa acgccgcttt tggcatatcc gggtgacgca ccatgttgtg cggctgccct
    74821 tgtaactctt gcaaggtata gccgctcacc tgcacaaaag tgtcattagc atgagtgata
    74881 tagctttgca gatcggtagt ggacatcaga gtggtatcgt ccgccagcgg ggtattttgc
    74941 tgggtgacat acggatgaga agacatgttc gcgtcctgtg caggttatat ggttgttaac
    75001 ttcttgtcag agtttatgtc ggccccgctg cggttatctt taaccgatta atttgattta
    75061 gatcgcaatt tgcgattaaa gcacaaatct aattcgttga tttaaaatac tttcactctg
    75121 ttactatacg aaaacgttaa ttatcttgcc caaaaatcag gcaattattg ccctgaaaac
    75181 gtgcatttgc gcagcaatta tcaaatccat acccgacaaa aaccgtgcaa aataacaacg
    75241 gatgttaaca aatagcatta aatattgcgc aaatgataac cacatttgtg tttatcccga
    75301 ttttcgcgat cgcagccgga gtggcgcaat ccctgcaata cttaaatcgg tatcatgtga
    75361 tacgcgagcc tccggagcat attttgaaca ggttaccttc gagcgcatcg gctttagcgt
    75421 gcagcgccca cgccctgaat ctcattgaga agcgaacgct ggatcatgag gagatgaaag
    75481 cacttaaccg agaggtgatt gaatacttca aagagcatgt caatccgggg tttttagagt
    75541 atcgcaaatc tgttaccgcc ggcggggatt acggagccgt agagtggcaa gcgggaggtt
    75601 taaatacgct tgtcgacacc cagggacagg agtttatcga ctgcctggga ggttttggaa
    75661 ttttcaacgt ggggcaccgt aatccagttg tggtttccgc cgtacagaat caacttgcga
    75721 aacaaccact gcacagccag gagctactcg atccgttacg ggctatgctg gcgaaaaccc
    75781 ttgctgcgct aacgcccggt aaactgaaat acagcttctt ctgtaatagc ggcaccgagt
    75841 ccgttgaagc ggcgctgaag ctggcgaagg cttatcagtc gccgcgcggc aaatttactt
    75901 ttattgccac cagcggcgcg ttccacggta aatcacttgg cgcgctgtcg gccacggcga
    75961 aatcgacctt ccgcaaaccg tttatgccac tgctgccagg gttccgtcat gtgccgtttg
    76021 gcaatatcga agccatgcgc acggctctta atgagtgcaa aaaaaccggt gatgatgtgg
    76081 ctgcggtgat cctcgaaccg attcagggcg aaggtggcgt aattctgccg ccgccgggct
    76141 atctcaccgc cgtgcgtaag ctgtgcgatg agttcggcgc actgatgatc ctcgatgaag
    76201 tacaaacggg catggggcgc acgggcaaga tgtttgcctg cgaacatgag aacgtccagc
    76261 cagacattct gtgtctggca aaagccctcg gcggcggcgt gatgccgatt ggcgcaacca
    76321 tcgctactga agaggtgttc tcagtcttgt tcgacaaccc gttcctgcat accaccacct
    76381 ttggcggcaa cccgctggcc tgtgcggcgg cgctggcgac catcaatgtg ttgctggaac
    76441 aaaacttacc ggctcaggct gaacaaaaag gcgatatgtt gctggacggt ttccgtcaac
    76501 tggcgcggga atatcccgat ctggtgcagg aagcacgtgg taaagggatg ttgatggcga
    76561 ttgagtttgt tgataacgaa atcggctata actttgccag cgagatgttc cgccagcgcg
    76621 tactggtggc cggaacgctc aataacgcca aaacgatccg cattgaaccg ccactgacac
    76681 tgaccattga acagtgtgaa ctggtgatca aagcggcgcg taaggcgctg gcggccatgc
    76741 gagtaagtgt cgaagaagcg taatatcaaa tcggatggcg atacgacgtc gccatccgat
    76801 ctttttatac gatacgtacg cccgcaggca tcatccgctc cggcgttaac agcacgcttt
    76861 cactgccgtc atcggtttcc gcacacaaca gcatgcattc cgacgtttca ccgcgcattt
    76921 tcgctttttg cagattgcat aagaccacca ccgtttttcc catcaactct tcttcgctgt
    76981 agtacggcac caggctggtc acggtttgca gtgttttttc ccccacatca acctgtacga
    77041 tgtacagctt gtcggcgttt tcatggcgtt tcacttccac aatctttccg acgcgcattt
    77101 ccagacgtgc aaaatcagcg taagccacgg tttccattgc ttcctccctt cgagtaaaat
    77161 tttactaaac tatagcaaaa gtttttctca atcctgtagg ctaaaaatgg agaatgcagg
    77221 cgtgatcaca ttcgtgagcc gctgtgttac cgttacagcg tcaaagaaac gcgctttatt
    77281 tactgaaaac aggtgacccg ataagcactt cctctacaat gggggcgcac atcagggaaa
    77341 gtaaaaaagg taaacatggc aacactaaaa gacatcgcaa tcgaagctgg cgtatccctg
    77401 gcgacagtat ccagggtctt aaatgacgat ccgacattga atgtgaaaga agagacgaaa
    77461 catcgcattc tcgagatcgc cgaaaagctg gagtacaaga ccagtagcgc ccgtaaacta
    77521 cagacaggcg cagtcaacca acaccatatt ctggctatct acagctacca gcaggagctg
    77581 gagatcaacg atccttacta tctggcgatc cgccacggca ttgaaactca gtgcgaaaag
    77641 ctggcgatcg aactgaccaa ctgttatgaa cacagcggat taccagacat taaaaacgtc
    77701 accggtattt taattgtcgg caaacccaca cccgccctgc gcgccgccgc ctgtgcgttg
    77761 accgacaata tctgttttat cgactttcac gaacccggca gcggttacga tgcggtggat
    77821 atcgatctgg cacgcatcag taaagaaatc atcgacttct atatcaacca gggcgttaat
    77881 cgcattggtt ttattggcgg tgaagatgag cctggcaagg cggatattcg tgaggtcgcc
    77941 tttgcggaat atggccgtct gaaacaagta gtacgcgaag aggatatctg gcgcggcggt
    78001 ttttccagtt cgtcgggtta tgaactggca aaacagatgc tggcgcggga agactatccg
    78061 aaggcactgt ttgttgcttc cgattccatt gctatcggcg tactgcgggc aattcatgaa
    78121 cgtggcctga acatcccaca ggatatttcg cttatcagcg ttaacgatat ccccaccgcg
    78181 cgatttacct ttccgccgct ctccaccgtg cgcatccatt ccgagatgat gggaagtcag
    78241 ggcgttaacc tggtgtatga aaaagcccgc gatggtcgcg cgctgccgct gttagtcttc
    78301 gttcccagca aattaaaact gcgcggcacg acccgttaaa tccccttaca cactgtccgg
    78361 caatcatttt tgccggacag tgctgccgtt tattttcgtg atccagttaa agtaaatgca
    78421 tttacctgct attttttagt aaaaatttta ctaaactccc cagcaattac acaaactacc
    78481 atcaccatga atggttccga tttctctcta ccgggaggcc ctatgaatcg ctgggaaaac
    78541 attcagctca cccacgaaaa ccgacttgcg ccgcgtgcgt actttttttc atatgattct
    78601 gttgcgcagg cgcgtacctt tgcccgcgaa acgagcagcc tgtttctgcc cttaagcggt
    78661 cagtggaatt tccacttttt tgaccatccg ctgcaagtgc cagaagcctt cacctctgag
    78721 ttaatggctg actgggggca tattaccgtc cccgccatgt ggcaaatgga aggtcacggc
    78781 aaactgcaat ataccgacga aggttttccg ttccccatcg atgtgccgtt tgttcccagc
    78841 gataacccaa ccggtgccta tcaacgtatt ttcaccctca gtgacggctg gcagggtaaa
    78901 cagacgctga ttaaatttga cggcgtcgaa acctattttg aagtctacgt taacggtcag
    78961 tatgtgggtt tcagcaaggg cagtcgcctg accgcagagt ttgacatcag cgcgatggtt
    79021 aaaaccggcg acaacctgtt gtgtgtgcgc gtgatgcagt gggcggactc tacctacgtg
    79081 gaagaccagg atatgtggtg gtcggcgggg atcttccgcg atgtttatct ggtcggaaaa
    79141 caactaacac atattaacga tttcaccgtg cgtaccgact ttgacgaagc ctattgcgat
    79201 gccacgcttt cctgcgaagt ggtgctagaa aatctcgccg cctcccctgt cgtcacgacg
    79261 ctggaatata ccctgtttga tggcgaacgc gtggtgcaca gcagcgccat tgatcatttg
    79321 gcaattgaaa aactgaccag cgccagcttt gcttttactg tcgaacagcc acagcaatgg
    79381 tcagcagaat ccccttatct ttaccatctg gtcatgacgc tgaaagacgc caacggcaac
    79441 gttctggaag tggtaccaca acgcgttggc ttccgtgata tcaaagtgcg cgacggtctg
    79501 ttctggatca ataaccgtta tgtgatgctg cacggcgtca accgtcacga caacgatcat
    79561 cgcaaaggcc gcgccgttgg aatggatcgc gtcgagaaag atctccagtt gatgaagcag
    79621 cacaacatca actccgtgcg taccgctcac tacccgaacg atccgcgttt ttacgaactg
    79681 tgtgatatct acggcttgtt tgtgatggcg gaaaccgacg tcgaatcgca cggctttgct
    79741 aatgtcggcg atatcagccg tattaccgac gatccgcagt gggaaaaagt ctacgtcgag
    79801 cgcattgttc gccatattca cgcgcagaaa aaccatccgt cgatcatcat ctggtcgctg
    79861 ggcaatgaat ccggctatgg ctgtaacatc cgcgcgatgt accacgcagc gaaagcgctg
    79921 gatgacacgc gactggtgca ttacgaagaa gatcgcgatg ctgaagtggt cgatattatt
    79981 tccaccatgt acacccgcgt gccgctgatg aatgagtttg gtgaataccc gcatccgaag
    80041 ccgcgcatca tctgtgaata tgctcatgcg atggggaacg ggccgggcgg gctgacggag
    80101 taccagaacg tcttctataa gcacgattgc attcagggac attatgtctg ggaatggtgc
    80161 gaccacggga tccaggcgca ggatgacaac ggcaatgtct ggtataaatt cggcggcgac
    80221 tacggcgact atcccaacaa ctataacttc tgtcttgatg gtttgatcta ttccgatcag
    80281 acgccgggac caggcctgaa agagtacaaa caggttattg cgccggtaaa aatccacgcg
    80341 ctggatctga ctcgcggcga gttgaaagtc gaaaataaac tgtggtttac cacgcttgat
    80401 gactacaccc tgcacgtaga agtgcgcgcc gaaggtgaaa cgctcacgac gcggcagatt
    80461 aaactgcgcg acgttgcgcc gaacagcgaa gcccccttgc agatcacgct gccgcagctg
    80521 gacgcccgcg aagcgttcct caacattacg gtgaccaaag attcccgcac ccgctacagc
    80581 gaagccgggc attctatcgc cacttatcag ttcccgctga aggaaaacac cgcgcagcca
    80641 gtgcctttcg caccaaataa tgcgcgtccg ctgacgctgg aagacgatcg tttgagctgc
    80701 accgttcgcg gctacaactt cgcgatcacc ttctcaaaaa tgagtggcaa accgacatcc
    80761 tggcaggtga atggcgagtc gctgctgacc cgcgagccaa agatcaactt cttcaagcca
    80821 atgatcgaca accacaagca ggagtacgaa gggctgtggc aaccgaatca tttgcagatc
    80881 atgcaggaac atctgcgcga ctttgccgta gaacagagcg atggtgaagt gttgatcatc
    80941 agccgcacgg ttatagcacc gccggtgttt gacttcggga tgcgctgcac ctacatctgg
    81001 cgcatcgctg ccgatggcca ggttaacgtg gcgctttccg gcaagcgtta cggcgactat
    81061 ccgcacatca ttccgtgcat cggtttcacc atggggatta acggcgaata cgatcaggtg
    81121 gcgtattacg gtcgtggacc gggcgaaaac tacgccgaca gccagcaggc taacatcatc
    81181 gatatctggc gcagcaccgt cgatgccatg ttcgagaact atcccttccc gcagaacaac
    81241 ggtaaccgtc agcatgtccg ctggacggca ctgactaacc gccacggcaa cggtctgctg
    81301 gtggttccgc agcgcccaat taacttcagc gcctggcact atacccagga aaacatccac
    81361 gctgcccagc actgtaacga gctgcagcgc agtgatgata tcaccctgaa tctcgaccac
    81421 cagctgcttg gcctcggttc caattcctgg ggcagcgagg tgctggactc ctggcgcgtc
    81481 tggttccgtg acttcagcta cggctttacg ttgctgccgg tttctggcgg agaagctacc
    81541 gcgcaaagcc tggcgtcgta tgagttcggc gcagggttct tttccacgaa tttgcacagc
    81601 gagaataagc aatgaggatc atcgataact tagaacagtt ccgccagatt tacgcctctg
    81661 gtaagaagtg gcaacgctgc gttgaagcga ttgaaaatat cgacaacatt cagcctggcg
    81721 tcgcccactc catcggtgac tcattgactt accgcgtgga gacagactcc gcgaccgatg
    81781 cgctatttac cgggcatcga cgctattttg aagtgcatta ctacctgcaa gggcagcaaa
    81841 aaattgaata tgcgccgaaa gagacattac aggtagtgga atattatcgt gacgaaactg
    81901 accgtgaata tttaaaaggc tgcggagaaa ccgttgaggt ccacgaagga caaatcgtta
    81961 tttgcgatat ccatgaagca tatcggttta tctgcaataa cgcggtcaaa aaagtggttc
    82021 tcaaagtcac catcgaagat ggttattttc ataacaaata acaactacgg cggcaaaagg
    82081 agtttgccgc caccgctacc ctactcattt tcggagatgt gttatgtctg ataccaaacg
    82141 taatacaatc ggcaaattcg gcttgctctc gctgactttt gccgccgttt acagctttaa
    82201 caacgttatc aataataata ttgagcttgg actggcctcg gcaccgatgt ttttcctcgc
    82261 gacgattttt tattttattc ccttctgtct gatcatcgca gaatttgttt cgttaaataa
    82321 aaactcagaa gccggtgtct acgcgtgggt aaaaagttcg ctgggcggac gttgggcatt
    82381 tattactgcc tatacctact ggttcgtaaa cctgttcttt ttcacctcgc tgttgccgcg
    82441 cgttattgct tatgcttcgt atgccttcct cggctacgaa tatattatga cgccggttgc
    82501 caccaccatt atcagtatgg tgctgttcgc cttctctacc tgggtttcca ccaacggggc
    82561 gaaaatgctg gggccaatta cctccgtcac ttcaacgctg atgctgctgt taacgctctc
    82621 ctacatttta ctggcaggta cggcgctggt tggcggcgta cagcctgctg accccatcac
    82681 cgttgacgcg atgatcccga acttcaactg ggcgttcctc ggcgttacca cctggatttt
    82741 tatggccgca ggtggcgcgg agtccgtcgc tgtgtacgtt aacgacgtca aaggcggttc
    82801 gaaatcgttc gttaaagtga tcatcctcgc cgggattttt atcggcgtac tatattccgt
    82861 ctcctcggtg ctgattaacg tcttcgtcag cagcaaagag ttgaaattta ctggcggatc
    82921 ggtgcaggta ttccacggca tggcggcgta ttttggtctg ccggaagcgt tgatgaatcg
    82981 ctttgtcggt ctggtgtcct ttaccgcaat gttcggttcc ctgctgatgt ggaccgcaac
    83041 gccggtgaaa attttcttct ccgaaatccc ggaaggcatc tttggtaaga aaaccgtaga
    83101 actgaacgaa aacggcgttc cggcgcgcgc agcgtggatc cagttcctga tcgtcatccc
    83161 gctgatgatt atcccgatgc tcggttccaa taccgtgcag gatctgatga ataccattat
    83221 taatatgacc gccgcagcgt ccatgcttcc gccgttattc atcatgctgg cttacctgaa
    83281 tttacgcgcc aaattagatc acctgccacg cgatttccgt atgggctccc gccgcaccgg
    83341 tattatcgtt gtttcaatgc tgattgcgat atttgccgta gggtttgtcg cttcgacatt
    83401 cccgactggc gcgaatattc tgaccatcat tttttataac gtcggcggta ttgttatctt
    83461 ccttggcttt gcgtggtgga aatacagtaa atatataaag ggattaacgg ctgaagagcg
    83521 ccatattgaa gcgacgccag ccagcaatgt tgattaaaca gactaataaa aaatgagcag
    83581 gcattttccc tcttcctgga tgaggagagg gaaaatagtt tctgccttta tatttttata
    83641 attacaacga taaaaggctg tactttttct ttagctcatg gattaacaca atgaaattaa
    83701 tcactgcacc atgcagagca ttacttgctc tgccgttttg ctacgccttt tctgcggcag
    83761 gagaagaagc acgtccggca gaacatgacg acacaaaaac acccgcaatt acctcgacat
    83821 cttctccttc atttcgtttt tacggcgaat taggggttgg tggatatatg gatttagagg
    83881 gtgagaataa acataaatac agcgacggca cctatattga aggtggcctg gagatgaagt
    83941 acggctcctg gttcggcctg atttacggcg aaggctggac cgtgcaggcc gaccacgacg
    84001 gcaatgcctg ggtgcctgac catagctggg gcggtttcga aggtgggatt aaccgtttct
    84061 atggcggtta tcgaaccaat gatggcaccg aaatcatgct cagtctgcgt caggattcct
    84121 cgctggatga cctgcaatgg tggggcgact tcacacccga tctgggctac gtcattccca
    84181 atacccgcga cattatgacc gcgctgaagg tgcagaactt aagcggcaac tttcgttata
    84241 gcgtcaccgc cacacctgcc ggacatcatg acgaaagcaa agcatggcta cattttggca
    84301 agtacgatcg ctacgacgac aaatacacct atccggcaat gatgaacggt tacatccagt
    84361 atgaccttgc cgaaggcatc acctggatga atggtctgga aattaccgac ggcacaggac
    84421 aactctatct cacgggcctg cttaccccta actttgccgc tcgcgcctgg caccataccg
    84481 gacgtgccga cgggttggac gtaccgggaa gtgaaagcgg gatgatggtg agcgccatgt
    84541 atgaagcgtt aaagggcgtt tatctctcca ccgcttacac ctacgccaaa catcgccctg
    84601 accacgctga cgatgaaacc acctctttca tgcagtttgg tatctggtac gaatatggcg
    84661 gcggacgttt cgcaacggct tttgatagcc gcttctacat gaaaaatgcc tctaacgatc
    84721 ccagcgacca aatcttcctg atgcaatatt tctactggta ataaggactg taatttcatg
    84781 aaaatcaaaa ctattttaac gccagtaacc tgcgctctgc tgataagttt ttccgcccat
    84841 gccgctaacg ccgacaatta taaaaacgtg attaaccgta ctggtgcgcc gcagtacatg
    84901 aaggattacg attacgacga tcaccagcgt tttaatccgt ttttcgatct cggagcctgg
    84961 catggtcatc tgttgccaga cggccctaac accatgggcg gttttccagg cgttgcgctg
    85021 ctgacggaag agtacatcaa ctttatggcc agcaatttcg accgcctgac cgtctggcag
    85081 gacggcaaga aagtcgactt cacgctggag gcatacagta ttcccggcgc gctggtgcaa
    85141 aaactgacag caaaagatgt gcaggtcgaa atgactctgc gcttcgccac gccgcgcacg
    85201 tcactactgg aaactaaaat catcagcaat aaaccgctgg atctggtgtg ggatggcgaa
    85261 ctgctggaaa aactggaagc gaaagaaggg aaaccgcttt ccgataaaac cattgctggc
    85321 gaataccctg actaccagcg caaaatcagc gccacccgtg atggcctgaa agtcaccttt
    85381 ggcaaagtgc gcgccacttg ggatctgctg acctccggtg aatcagaata tcaggtgcat
    85441 aaatccctgc cggtgcagac tgaaatcaac ggtaatcgct ttaccagtaa ggcgcatatc
    85501 aacgggtcga cgacgctcta taccacctac tcccacctgc tgaccgctca ggaagttagc
    85561 aaagagcaaa tgcagatccg cgatattctg gcgcgtccgg cgttttacct caccgcctcg
    85621 cagcaacgct gggaagagta tctgaagaaa gggttaacca atccggatgc gacgccggaa
    85681 cagacgcgcg tcgcagtaaa agcaatcgaa acgctcaacg gtaactggcg ttcgcctggc
    85741 ggggcggtga aatataacac cgtcacgccg tcggtgaccg ggcgctggtt ctccggcaat
    85801 cagacctggc cgtgggatac ctggaagcag gcgtttgcga tggcgcattt caatccggac
    85861 atcgccaaag agaatatccg cgcggtcttc tcctggcaga tccagcctgg cgacagcgtg
    85921 cgtccgcagg atgtgggctt tgtccccgac ctgatagcgt ggaaccttag ccccgagcgt
    85981 ggtggcgatg gcggcaactg gaacgaacgt aataccaaac ccagccttgc cgcctggtcg
    86041 gtgatggaag tgtataacgt cacccaggat aaagcctggt tggcagagat gtacccgaaa
    86101 ctggtggcct atcatgactg gtggttacgt aaccgcgatc ataacggcaa cggcgtgccg
    86161 gaatatggcg cgacccgcga caaagcccac aacactgaga gcggcgagat gctgtttacg
    86221 gtgaagaaaa gcgacaaaga agagacgcag tctgggctga acaactacgc ccgcgtggtg
    86281 gagaaaggcc agtacgacag tctggaaatt ccggcacagg ttgctgcatc gtgggaatcg
    86341 gggcgtgatg atgccgccgt ctttgggttt atcgacaaag aacagctgga taaatatgtc
    86401 gcaagcggcg gcaaacgtag cgactggacg gtgaaattcg ccgaaaaccg cagtcaggac
    86461 ggaacgttgc tggggtactc gctattgcag gagtcggtgg atcaggccag ctatatgtac
    86521 agcgataacc attatctggc ggagatggcg acgattctcg gtaaaccgga agaggctaag
    86581 cgctatcgcc agttggcaca gcagctcgcg gactacatca acacctgtat gttcgacccg
    86641 gctacgcagt acttctatga cgtgcgtatt gaagataaac cgctggcgaa cggctgcgcg
    86701 ggcaaaccga ttgttgagcg cggtaaaggg ccggaaggct ggtcgccgct gtttaacggt
    86761 gcggcaacgc aggccaatgc cgacgcggtg gtgaaggtga tgctcgatcc taaagagttc
    86821 aatacctttg ttccgctggg aacggcggcg ttaaccaatc cggcttttgg cgctgatatc
    86881 tactggcgcg ggcgcgtatg ggtggatcag ttctggtttg gtctgaaagg gatggagcgt
    86941 tacggttatc gtgatgatgc cctgaagctg gcggatacgt tcttccggca tgccaaagga
    87001 ttaaccgccg atggcccaat tcaggagaat tacaacccgc tgactggcgc gcagcaaggc
    87061 gcaccaaatt tctcctggag tgccgcacat ttgtatatgt tgtataacga ttttttccgt
    87121 aagcaataat ttgcaacggc tggcggatgc ggcgtgaacg ccttatccgc cctacatatt
    87181 cgttcccgta ggctggataa gacgcgacaa gcgtcgcatc cggcatctgc accgcgccac
    87241 cggcggatgc tgcgtgaacg ccttatccgc cctacatgtt cgttcccgta ggctggataa
    87301 gacgcgacaa gcgtcgcatc cggcatctgc acctcgccac tggcggatgc ggcgtgaacg
    87361 ccttatccgc cctacatgtt cgttcccgta ggctggataa gacccgacaa gcgtcgcatc
    87421 cggcgtctgc accgcgccac tggcggatgc ggcgtgaacg ccttatccgc cctacatgtt
    87481 cgttcccgta ggctggataa gacgcgacaa gcgtcgcatc cggcatctgc accgcgccgg
    87541 cggatgcggc gtgaacgcct tatccgccct acatgtgcaa tcccccaggt cggataggac
    87601 ccggcaagcg tcgcatccgg catctgcacc gcgccggcgg atgcggcgtg aacgccttat
    87661 ccgccctaca tgttcgttcc cgtaggctgg ataagacgcg acaagcgtcg tatccggcat
    87721 ctgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca tgtgcaatcc
    87781 cccaggtcgg ataggacccg gcaagcgtcg catccggcgt taccaccggg cgtattcttt
    87841 ttgaatccca tcacaaaccc cgcactcccc ttttcccttt tctccggcga cggctaaatt
    87901 agaactcatc cgaccacata acaattattt tacatactgg acaattttat gagctacccg
    87961 tcgctgttcg ccccgctgga tttaggtttt accacgttaa aaaaccgcgt gttgatgggt
    88021 tcaatgcaca ccgggctgga ggaatacccg gacggcgctg aacggctggc tgcgttttat
    88081 gccgaacgcg cccgtcacgg cgtggcgctg attgttagcg gcggcattgc accagattta
    88141 acaggcgttg gcatggaagg cggcgcaatg ctcaatgacg ccagccagat cccacaccat
    88201 cgcaccatta ccgaagccgt acatcaggaa ggcggcaaaa tagccctgca aattttgcat
    88261 accgggcgct acagctacca accgcatctg gtcgcaccgt ccgcattgca ggcccccatc
    88321 aaccgtttcg ttcctcatga actcacccat gaagagatcc tgcaactgat cgacgatttc
    88381 gcccgctgcg cgcaactggc gcgggaggca ggatacgacg gtgtagaggt gatgggttcc
    88441 gaagggtatt tgatcaacga atttctgacg ctgcgcacca atcagcgtag tgaccagtgg
    88501 ggcggcgatt accacaaccg gatgcggttt gccgttgaag tggtgagtgc ggtacgcgaa
    88561 cgcgttggca acgacttcat tattatctac cgactgtcga tgctcgacct ggtcgaagac
    88621 ggcgggactt ttgccgaaac ggtagagctg gcgcaggcca ttgaagcggc gggcgcaacc
    88681 attatcaaca ccggcattgg ctggcatgaa gcacgtattc cgaccattgc cacgcccgtg
    88741 ccgcgcggcg catttagctg ggtcacgcgc aaactgaaag gccacgtttc gctgccgctg
    88801 gtgaccacca accgaattaa cgatccgcag gttgccgacg acattctctc gcgcggcgat
    88861 gccgatatgg tgtcgatggc acgaccgttt cttgctgatg cggagctgct gtcaaaagcg
    88921 caatcagggc gagccgatga gatcaacacc tgcattggct gcaatcaggc ctgcctcgat
    88981 caaatcttcg ttggcaaagt cacctcgtgc ctggtgaatc ctcgtgcctg ccatgaaacc
    89041 aaaatgccga tccttcccgc catgcagaaa aaaaatctgg cggtggtcgg tgcgggacca
    89101 gctgggctgg cgtttgccat taacgcggcg gcgcgtgggc atcaggtaac attgtttgat
    89161 gcccacagcg agatcggcgg gcagtttaat atcgccaaac agatccccgg caaagaggag
    89221 ttttacgaaa cgctgcgcta ctaccggcgg atgatcgaag taacgggcgt gacgctgaaa
    89281 ctcaatcaca ccgtaacagc gaatcagtta caggaattcg atgaaacgat cctcgccagt
    89341 gggatcgtgc cgcgcactcc gcccatcgac gggatcgatc atccgaaggt attgagttat
    89401 ctcgatgtac tgcgcgacaa agcgccggtg ggcaacaaag ttgccatcat cggttgtggc
    89461 gggattggtt ttgatacggc gatgtattta agtcagccgg gcgaatccac cagccagaat
    89521 atcgctgagt tttgtaatga gtgggggatc gacagtagcc tacaacaggc tggtggctta
    89581 agcccgcagg gaatgcagat cccgcgtagc ccacggcaga ttgtgatgct ccagcgcaaa
    89641 gccagcaaac cgggacaggg gttaggcaaa accaccggct ggatccatcg caccaccctg
    89701 ctctcgcggg gtgtgaaaat gatcccaggc gtaagttatc agaagattga cgatgacggg
    89761 ctgcatgtgg tgatcaacgg cgaaacgcag gtattagcag tggacaatgt ggtgatctgc
    89821 gcagggcaag agccaaaccg cgcgctggcg cagccgctga ttgatagcgg gaaaacggtg
    89881 catttaattg gcggctgcga tgtggctatg gagctggacg cacgacgggc aattgcccag
    89941 ggaacacggc tggcgctgga ga
//
LOCUS       DAZGBC010000002        89198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGBC010000002 DAZGBC010000000
VERSION     DAZGBC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.002"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(314..859)
                     /gene="hemG"
                     /locus_tag="ACHK1J_000085"
     CDS             complement(314..859)
                     /gene="hemG"
                     /locus_tag="ACHK1J_000085"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO7909433.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            complement(871..2322)
                     /gene="trkH"
                     /locus_tag="ACHK1J_000086"
     CDS             complement(871..2322)
                     /gene="trkH"
                     /locus_tag="ACHK1J_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO7909434.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFRQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2361..2975)
                     /gene="yigZ"
                     /locus_tag="ACHK1J_000087"
     CDS             complement(2361..2975)
                     /gene="yigZ"
                     /locus_tag="ACHK1J_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO7909435.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2975..4306)
                     /gene="pepQ"
                     /locus_tag="ACHK1J_000088"
     CDS             complement(2975..4306)
                     /gene="pepQ"
                     /locus_tag="ACHK1J_000088"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO7909436.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4496..6685
                     /gene="fadB"
                     /locus_tag="ACHK1J_000089"
     CDS             4496..6685
                     /gene="fadB"
                     /locus_tag="ACHK1J_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO7909437.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLVEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6695..7858
                     /gene="fadA"
                     /locus_tag="ACHK1J_000090"
     CDS             6695..7858
                     /gene="fadA"
                     /locus_tag="ACHK1J_000090"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO7909438.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8050..8751)
                     /gene="fre"
                     /locus_tag="ACHK1J_000091"
     CDS             complement(8050..8751)
                     /gene="fre"
                     /locus_tag="ACHK1J_000091"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO7909439.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8797..10290)
                     /gene="ubiD"
                     /locus_tag="ACHK1J_000092"
     CDS             complement(8797..10290)
                     /gene="ubiD"
                     /locus_tag="ACHK1J_000092"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO7909440.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10457..10945
                     /gene="rfaH"
                     /locus_tag="ACHK1J_000093"
     CDS             10457..10945
                     /gene="rfaH"
                     /locus_tag="ACHK1J_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO7909441.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10942..11724)
                     /gene="tatD"
                     /locus_tag="ACHK1J_000094"
     CDS             complement(10942..11724)
                     /gene="tatD"
                     /locus_tag="ACHK1J_000094"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO7909442.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11766..12542)
                     /gene="tatC"
                     /locus_tag="ACHK1J_000095"
     CDS             complement(11766..12542)
                     /gene="tatC"
                     /locus_tag="ACHK1J_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO7909443.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12545..13060)
                     /gene="tatB"
                     /locus_tag="ACHK1J_000096"
     CDS             complement(12545..13060)
                     /gene="tatB"
                     /locus_tag="ACHK1J_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO7909444.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13064..13333)
                     /gene="tatA"
                     /locus_tag="ACHK1J_000097"
     CDS             complement(13064..13333)
                     /gene="tatA"
                     /locus_tag="ACHK1J_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO7909445.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13412..15052)
                     /gene="ubiB"
                     /locus_tag="ACHK1J_000098"
     CDS             complement(13412..15052)
                     /gene="ubiB"
                     /locus_tag="ACHK1J_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO7909446.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15049..15654)
                     /gene="ubiJ"
                     /locus_tag="ACHK1J_000099"
     CDS             complement(15049..15654)
                     /gene="ubiJ"
                     /locus_tag="ACHK1J_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO7909447.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYIGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15668..16423)
                     /gene="ubiE"
                     /locus_tag="ACHK1J_000100"
     CDS             complement(15668..16423)
                     /gene="ubiE"
                     /locus_tag="ACHK1J_000100"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO7909448.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16518..17945)
                     /gene="rmuC"
                     /locus_tag="ACHK1J_000101"
     CDS             complement(16518..17945)
                     /gene="rmuC"
                     /locus_tag="ACHK1J_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO7909449.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINSDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18086..18847)
                     /gene="udp"
                     /locus_tag="ACHK1J_000102"
     CDS             complement(18086..18847)
                     /gene="udp"
                     /locus_tag="ACHK1J_000102"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO7909450.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            19109..19924
                     /gene="ysgA"
                     /locus_tag="ACHK1J_000103"
     CDS             19109..19924
                     /gene="ysgA"
                     /locus_tag="ACHK1J_000103"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO7909451.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGSQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            complement(19964..22225)
                     /gene="metE"
                     /locus_tag="ACHK1J_000104"
     CDS             complement(19964..22225)
                     /gene="metE"
                     /locus_tag="ACHK1J_000104"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO7909452.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            22461..23414
                     /gene="metR"
                     /locus_tag="ACHK1J_000105"
     CDS             22461..23414
                     /gene="metR"
                     /locus_tag="ACHK1J_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO7909453.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(23302..24201)
                     /gene="bioP"
                     /locus_tag="ACHK1J_000106"
     CDS             complement(23302..24201)
                     /gene="bioP"
                     /locus_tag="ACHK1J_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO7909454.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(24277..25077)
                     /gene="yigL"
                     /locus_tag="ACHK1J_000107"
     CDS             complement(24277..25077)
                     /gene="yigL"
                     /locus_tag="ACHK1J_000107"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO7909455.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(25085..26107)
                     /gene="pldB"
                     /locus_tag="ACHK1J_000108"
     CDS             complement(25085..26107)
                     /gene="pldB"
                     /locus_tag="ACHK1J_000108"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO7909456.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            26218..26838
                     /gene="rhtB"
                     /locus_tag="ACHK1J_000109"
     CDS             26218..26838
                     /gene="rhtB"
                     /locus_tag="ACHK1J_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO7909457.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(26900..27520)
                     /gene="rhtC"
                     /locus_tag="ACHK1J_000110"
     CDS             complement(26900..27520)
                     /gene="rhtC"
                     /locus_tag="ACHK1J_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO7909458.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(27584..29413)
                     /gene="recQ"
                     /locus_tag="ACHK1J_000111"
     CDS             complement(27584..29413)
                     /gene="recQ"
                     /locus_tag="ACHK1J_000111"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO7909459.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(29546..30415)
                     /gene="pldA"
                     /locus_tag="ACHK1J_000112"
     CDS             complement(29546..30415)
                     /gene="pldA"
                     /locus_tag="ACHK1J_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO7909460.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            30580..31047
                     /gene="yigI"
                     /locus_tag="ACHK1J_000113"
     CDS             30580..31047
                     /gene="yigI"
                     /locus_tag="ACHK1J_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO7909461.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            31099..31989
                     /gene="rarD"
                     /locus_tag="ACHK1J_000114"
     CDS             31099..31989
                     /gene="rarD"
                     /locus_tag="ACHK1J_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO7909462.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            32084..32464
                     /gene="yigG"
                     /locus_tag="ACHK1J_000115"
     CDS             32084..32464
                     /gene="yigG"
                     /locus_tag="ACHK1J_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFO7909463.1"
                     /translation="MLRMFIPTSNGKISRRRYIFSFILINFIFSFLIIFFNDGEAGFL
                     VIVSTIVLHYLVINMNCQRLRDSGFIYIKTYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            32478..32858
                     /gene="yigF"
                     /locus_tag="ACHK1J_000116"
     CDS             32478..32858
                     /gene="yigF"
                     /locus_tag="ACHK1J_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFO7909464.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(32901..33851)
                     /gene="corA"
                     /locus_tag="ACHK1J_000117"
     CDS             complement(32901..33851)
                     /gene="corA"
                     /locus_tag="ACHK1J_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO7909465.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(34003..34059)
                     /gene="ysgD"
                     /locus_tag="ACHK1J_000118"
     CDS             complement(34003..34059)
                     /gene="ysgD"
                     /locus_tag="ACHK1J_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO7909466.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            34221..34985
                     /gene="yigE"
                     /locus_tag="ACHK1J_000119"
     CDS             34221..34985
                     /gene="yigE"
                     /locus_tag="ACHK1J_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO7909467.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYVKGGAIPWQRYPFVTMISAERKG"
     gene            35068..35970
                     /locus_tag="ACHK1J_000120"
     CDS             35068..35970
                     /locus_tag="ACHK1J_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO7909468.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTGRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAISFEQVGPELPAKVVELPYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSEKKYEGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(36015..38177)
                     /gene="uvrD"
                     /locus_tag="ACHK1J_000121"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(36015..38177)
                     /gene="uvrD"
                     /locus_tag="ACHK1J_000121"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO7909469.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(38261..38977)
                     /gene="yigB"
                     /locus_tag="ACHK1J_000122"
     CDS             complement(38261..38977)
                     /gene="yigB"
                     /locus_tag="ACHK1J_000122"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO7909470.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(38977..39873)
                     /gene="xerC"
                     /locus_tag="ACHK1J_000123"
     CDS             complement(38977..39873)
                     /gene="xerC"
                     /locus_tag="ACHK1J_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO7909471.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(39870..40577)
                     /gene="yigA"
                     /locus_tag="ACHK1J_000124"
     CDS             complement(39870..40577)
                     /gene="yigA"
                     /locus_tag="ACHK1J_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO7909472.1"
                     /translation="MKQPREELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(40574..41398)
                     /gene="dapF"
                     /locus_tag="ACHK1J_000125"
     CDS             complement(40574..41398)
                     /gene="dapF"
                     /locus_tag="ACHK1J_000125"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO7909473.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLAYRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(41435..41638)
                     /gene="yifL"
                     /locus_tag="ACHK1J_000126"
     CDS             complement(41435..41638)
                     /gene="yifL"
                     /locus_tag="ACHK1J_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7909474.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            41797..41939
                     /locus_tag="ACHK1J_000127"
                     /pseudo
     CDS             41797..41939
                     /locus_tag="ACHK1J_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            42101..42421
                     /gene="cyaY"
                     /locus_tag="ACHK1J_000128"
     CDS             42101..42421
                     /gene="cyaY"
                     /locus_tag="ACHK1J_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO7909475.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VRFR"
     gene            complement(42461..45007)
                     /gene="cyaA"
                     /locus_tag="ACHK1J_000129"
     CDS             complement(42461..45007)
                     /gene="cyaA"
                     /locus_tag="ACHK1J_000129"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO7909476.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPDRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            45394..46335
                     /gene="hemC"
                     /locus_tag="ACHK1J_000130"
     CDS             45394..46335
                     /gene="hemC"
                     /locus_tag="ACHK1J_000130"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO7909477.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            46332..47072
                     /gene="hemD"
                     /locus_tag="ACHK1J_000131"
     CDS             46332..47072
                     /gene="hemD"
                     /locus_tag="ACHK1J_000131"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO7909478.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            47094..48299
                     /gene="hemX"
                     /locus_tag="ACHK1J_000132"
     CDS             47094..48299
                     /gene="hemX"
                     /locus_tag="ACHK1J_000132"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO7909479.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMNNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQAAPQAAPQAD
                     TPAAAPQGE"
     gene            48302..49498
                     /gene="hemY"
                     /locus_tag="ACHK1J_000133"
     CDS             48302..49498
                     /gene="hemY"
                     /locus_tag="ACHK1J_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO7909480.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            50177..51832
                     /gene="aslA"
                     /locus_tag="ACHK1J_000134"
     CDS             50177..51832
                     /gene="aslA"
                     /locus_tag="ACHK1J_000134"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO7909481.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     DYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(52000..53235)
                     /gene="aslB"
                     /locus_tag="ACHK1J_000135"
     CDS             complement(52000..53235)
                     /gene="aslB"
                     /locus_tag="ACHK1J_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7909482.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRYYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(53382..53458)
                     /locus_tag="ACHK1J_000136"
     tRNA            complement(53382..53458)
                     /locus_tag="ACHK1J_000136"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53422..53424),aa:Pro,seq:tgg)
     gene            complement(53501..53587)
                     /locus_tag="ACHK1J_000137"
     tRNA            complement(53501..53587)
                     /locus_tag="ACHK1J_000137"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53551..53553),aa:Leu,seq:cag)
     gene            complement(53608..53683)
                     /locus_tag="ACHK1J_000138"
     tRNA            complement(53608..53683)
                     /locus_tag="ACHK1J_000138"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53648..53650),aa:His,seq:gtg)
     gene            complement(53742..53818)
                     /locus_tag="ACHK1J_000139"
     tRNA            complement(53742..53818)
                     /locus_tag="ACHK1J_000139"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53782..53784),aa:Arg,seq:ccg)
     gene            complement(53921..55306)
                     /gene="yifK"
                     /locus_tag="ACHK1J_000140"
     CDS             complement(53921..55306)
                     /gene="yifK"
                     /locus_tag="ACHK1J_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7909483.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(55497..56237)
                     /gene="wecG"
                     /locus_tag="ACHK1J_000141"
     CDS             complement(55497..56237)
                     /gene="wecG"
                     /locus_tag="ACHK1J_000141"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO7909484.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(56240..57592)
                     /gene="wzyE"
                     /locus_tag="ACHK1J_000142"
     CDS             complement(56240..57592)
                     /gene="wzyE"
                     /locus_tag="ACHK1J_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO7909485.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILIGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(57589..58668)
                     /gene="rffT"
                     /locus_tag="ACHK1J_000143"
                     /gene_synonym="wecF"
     CDS             complement(57589..58668)
                     /gene="rffT"
                     /locus_tag="ACHK1J_000143"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO7909486.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDNCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKSR
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(58665..59915)
                     /gene="wzxE"
                     /locus_tag="ACHK1J_000144"
     CDS             complement(58665..59915)
                     /gene="wzxE"
                     /locus_tag="ACHK1J_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO7909487.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVIGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(59917..61047)
                     /gene="rffA"
                     /locus_tag="ACHK1J_000145"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(59917..61047)
                     /gene="rffA"
                     /locus_tag="ACHK1J_000145"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO7909488.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(61052..61726)
                     /gene="rffC"
                     /locus_tag="ACHK1J_000146"
                     /gene_synonym="wecD"
     CDS             complement(61052..61726)
                     /gene="rffC"
                     /locus_tag="ACHK1J_000146"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO7909489.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(61704..62585)
                     /gene="rfbA"
                     /locus_tag="ACHK1J_000147"
     CDS             complement(61704..62585)
                     /gene="rfbA"
                     /locus_tag="ACHK1J_000147"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO7909490.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(62604..63671)
                     /gene="rffG"
                     /locus_tag="ACHK1J_000148"
     CDS             complement(62604..63671)
                     /gene="rffG"
                     /locus_tag="ACHK1J_000148"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO7909491.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(63671..64933)
                     /gene="wecC"
                     /locus_tag="ACHK1J_000149"
     CDS             complement(63671..64933)
                     /gene="wecC"
                     /locus_tag="ACHK1J_000149"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO7909492.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(64930..66060)
                     /gene="wecB"
                     /locus_tag="ACHK1J_000150"
     CDS             complement(64930..66060)
                     /gene="wecB"
                     /locus_tag="ACHK1J_000150"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO7909493.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(66116..67162)
                     /gene="wzzE"
                     /locus_tag="ACHK1J_000151"
     CDS             complement(66116..67162)
                     /gene="wzzE"
                     /locus_tag="ACHK1J_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO7909494.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(67174..68277)
                     /gene="wecA"
                     /locus_tag="ACHK1J_000152"
     CDS             complement(67174..68277)
                     /gene="wecA"
                     /locus_tag="ACHK1J_000152"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO7909495.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(68517..69776)
                     /gene="rho"
                     /locus_tag="ACHK1J_000153"
     CDS             complement(68517..69776)
                     /gene="rho"
                     /locus_tag="ACHK1J_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO7909496.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(69861..69962)
                     /gene="rhoL"
                     /locus_tag="ACHK1J_000154"
     CDS             complement(69861..69962)
                     /gene="rhoL"
                     /locus_tag="ACHK1J_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO7909497.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(70103..70432)
                     /gene="trxA"
                     /locus_tag="ACHK1J_000155"
     CDS             complement(70103..70432)
                     /gene="trxA"
                     /locus_tag="ACHK1J_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO7909498.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            70563..71828
                     /gene="rhlB"
                     /locus_tag="ACHK1J_000156"
     CDS             70563..71828
                     /gene="rhlB"
                     /locus_tag="ACHK1J_000156"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO7909499.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            71962..73446
                     /gene="gppA"
                     /locus_tag="ACHK1J_000157"
     CDS             71962..73446
                     /gene="gppA"
                     /locus_tag="ACHK1J_000157"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO7909500.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(73493..75514)
                     /gene="rep"
                     /locus_tag="ACHK1J_000158"
     CDS             complement(73493..75514)
                     /gene="rep"
                     /locus_tag="ACHK1J_000158"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO7909501.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            75731..76180
                     /locus_tag="ACHK1J_000159"
     CDS             75731..76180
                     /locus_tag="ACHK1J_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO7909502.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFITTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNIINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            76375..76656
                     /gene="ppiC"
                     /locus_tag="ACHK1J_000160"
     CDS             76375..76656
                     /gene="ppiC"
                     /locus_tag="ACHK1J_000160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO7909503.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(76702..78177)
                     /gene="ilvC"
                     /locus_tag="ACHK1J_000161"
     CDS             complement(76702..78177)
                     /gene="ilvC"
                     /locus_tag="ACHK1J_000161"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO7909504.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            78327..79220
                     /gene="ilvY"
                     /locus_tag="ACHK1J_000162"
     CDS             78327..79220
                     /gene="ilvY"
                     /locus_tag="ACHK1J_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO7909505.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(79272..80816)
                     /gene="ilvA"
                     /locus_tag="ACHK1J_000163"
     CDS             complement(79272..80816)
                     /gene="ilvA"
                     /locus_tag="ACHK1J_000163"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO7909506.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(80819..82669)
                     /gene="ilvD"
                     /locus_tag="ACHK1J_000164"
     CDS             complement(80819..82669)
                     /gene="ilvD"
                     /locus_tag="ACHK1J_000164"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO7909507.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(82734..83663)
                     /gene="ilvE"
                     /locus_tag="ACHK1J_000165"
     CDS             complement(82734..83663)
                     /gene="ilvE"
                     /locus_tag="ACHK1J_000165"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO7909508.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(83683..83946)
                     /gene="ilvM"
                     /locus_tag="ACHK1J_000166"
     CDS             complement(83683..83946)
                     /gene="ilvM"
                     /locus_tag="ACHK1J_000166"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO7909509.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(83943..85589)
                     /gene="ilvG"
                     /locus_tag="ACHK1J_000167"
     CDS             complement(83943..85589)
                     /gene="ilvG"
                     /locus_tag="ACHK1J_000167"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO7909510.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLSTTKMPATCTLKGLGAVAADYSYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLSDEHAWRYDHPGDTIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(85592..85642)
                     /gene="ilvX"
                     /locus_tag="ACHK1J_000168"
     CDS             complement(85592..85642)
                     /gene="ilvX"
                     /locus_tag="ACHK1J_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO7909511.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(85729..85827)
                     /gene="ilvL"
                     /locus_tag="ACHK1J_000169"
     CDS             complement(85729..85827)
                     /gene="ilvL"
                     /locus_tag="ACHK1J_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO7909512.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            86180..87700
                     /gene="yifB"
                     /locus_tag="ACHK1J_000170"
     CDS             86180..87700
                     /gene="yifB"
                     /locus_tag="ACHK1J_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO7909513.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIANHL
                     QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSNIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(87725..88063)
                     /gene="maoP"
                     /locus_tag="ACHK1J_000171"
     CDS             complement(87725..88063)
                     /gene="maoP"
                     /locus_tag="ACHK1J_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO7909514.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            88182..89021
                     /gene="hdfR"
                     /locus_tag="ACHK1J_000172"
     CDS             88182..89021
                     /gene="hdfR"
                     /locus_tag="ACHK1J_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO7909515.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(89117..89192)
                     /locus_tag="ACHK1J_000173"
     tRNA            complement(89117..89192)
                     /locus_tag="ACHK1J_000173"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89157..89159),aa:Trp,seq:cca)
BASE COUNT        21710 a        24178 c        22637 g        20673 t
ORIGIN      
        1 attcattttt cgtcttgcga cgttaagaat ccgtatcttc gagtgcccac acagattgtc
       61 tgataaattg ttaaagagca gtgccgcttc gctttttctc agcggcgcgg ggtgtgcata
      121 atacactttc ccgctacaga gtcaagcatt tatttttgct ttctctgccg gaattctcag
      181 gagaatcccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg tggcgcatta
      241 tagggagtta ttccggcctg acaagaggaa atttaaaata attttctgac cgcgcatttt
      301 ttattcttta tgcttatttc agcgtcggtt tgtcggttaa atgggcgatt tctcgggcga
      361 aattcgccac ctgctcccaa tcggtataga caacttcttt gcgcgtatcc gtttcaccgc
      421 ctgacatctt cataatcagc ttgatcataa aacggtcgta ccagcgataa cgtgggtaac
      481 gcagcgcccc ggcaatgacc gcgcagcgat cgggacgcca ttgcgagttc atcagaaact
      541 tccgcgcgta gctgttggtc tgtggagtac gcttctccgg tttgcgcgcc accaggttta
      601 cggaataaaa ggcgctcggc atcgaattca gtcgcgtcgc atgttttttg acaaattcct
      661 ggaacgcaga gtggtagtga ccatagcgaa tagaagcacc aatgaccaca cggtcgtagt
      721 tttcccactg tggttcttca atgcggtgca cattggcgac atccgcctgg atccccagtt
      781 ctttcagttc cgaagccagg taggaggcaa tctcgcgcgt ttgtccgtcc cttgttgaga
      841 aaagaattaa tgttttcacg tattactcca tcattcacgc cagaaagtcg gggtaaagag
      901 caccagcaat gtaaagacct cgagacggcc aaacagcatg ttggcaatca ggatccattt
      961 agccaccggg ttcatgctgg taaagttatc agcaaccacg ccaagccctg gccccaggtt
     1021 attcaatgtc gcaacaaccg aggcaaaggc agaaaagtca tccacgcccg tggcgataat
     1081 cgccagcata ctgacaataa acaccaatgc ataggcggag aaaaatcccc agacggcttc
     1141 gaggatacgt tccggcagtg cgcgattccc cagcttaatg ctatagacgg cgttcggatg
     1201 caccagtcgt ttcagctcac ggttcccctg cctaaacagc agcaggatgc ggatcacttt
     1261 caggccaccg cccgttgacc cggcacaacc gccgataaaa gctgaacata aaagcagtac
     1321 cggcaaaaag agcggccagc gggcaatgct gtcagtcgta aacccggcgg ttgtcgccat
     1381 cgacaccacc tggaaaaacg cctggttaat tgtcatcagc gccgaactgt agacattatg
     1441 aaaccacagt acgagggtac aaataaccac cagcgtaaac tgtacgccga taaacatgcg
     1501 aaattccgga tcgcgccaat aaaccttcag actacgccca cttaacagtg aaaagtgcag
     1561 accgtagtta cagccggaga tcagcaggaa gatagcaatg atagtgttaa tagtcgggct
     1621 atcgaaataa ccgatactgg catcatgtgt cgagaagccg ccaatagcga tagtcgcaaa
     1681 gctatggccg atggcatcaa aggcatccat tccggcaaac cacaacgcca gcgcacaggc
     1741 gacggtcagc aagacataaa tcaaccacag ggttttcgcc gtttccgcaa ttcgcgggcg
     1801 cattttgtta tctttcagcg ggccgggcat ttctgcacga tagagctgca tcccacccac
     1861 gccgaggata ggcagtatcg caaccgctaa cacgatgatc cccatcccgc caaaccattg
     1921 cagcatctgg cgataaaaga ggatggcgtg agggagcgaa tccagcccca ccagcgtagt
     1981 ggccccggtg gtggtcaggc cagagaaaga ttcaaaaaac gcatcggtaa tcgtgaggtt
     2041 cgggctttcc gagaagataa aagggagcgc accgacacta cccagcacgg tccagaacag
     2101 caccactatc agaaacccct cacgggattt cagttcgccc ttctctttgc ggttcggcca
     2161 ccacagcata gagccaatgg cgagggcgac aaaaaaggtc tgggtaaaag cgcggcccgc
     2221 tccatcccgg tagatgagtg ctaccagccc agggataatc atggtccctg aaaataagat
     2281 gaccagtagt ccaacgattc gggtaatggc gcgaaaatgc atctctgccg cttccttagt
     2341 tctgcaaaac gaagtgggga ttattcttca atcgctaaca attgcaatga accacggcta
     2401 aaatccgcca gctttgcgga aaattcagcc actttcgccg ccggaagcgc cacccgcagc
     2461 agaacgaatg cctgataatc actgttgata attttgccgt cacactgccc cagcaacgct
     2521 tcaatgccgg ttaactgact atattcacat tgcaaagtat attcggttaa tggcgtcttg
     2581 cgttgggtcg ttagctgccg cagcgcctga ttcacgccgc cgccatacgc tttcactaac
     2641 ccaccggtgc caagcaatat gccgccgtag tagcgcacca ccacggcggt aatttccccg
     2701 acgccgctgc ccatcagttg ggcgagcatc ggtttacctg ccgttcccgc cggttccccg
     2761 tcgtcagaga aacccaactg ttgtgaatca tccggcgcac ccgccaccca cgccacacaa
     2821 tggtggcggg catcgggatg ttctgcccgc accgattcaa caaacgcttt cgccgcctca
     2881 acgccatcgg tatgcgccaa catcgttatg aagcggcttt ttttgatctc ttcaacgacc
     2941 gtgaccggtg ccgcaggaat taaccagctt tccatcacgc cagtttcaga tcccgggtca
     3001 tgttttccac gttattttcg tggatcacca cgttgtcttc gatacgaatg ccgccgaacg
     3061 gtttcagtgc ttcaattttc tgccagttga agtgcttgct gaactgccct tcacgccacg
     3121 gtgccagtag cgattcaatg aagtagatac ccggttcgat ggttaacacc atgcccggct
     3181 ggagaatacg ggtgcagcgc aggtacggat attttgccgg tgccgcgagg tgcgtaccgc
     3241 tatcatcctg cataaaaccg gcgacgtcat gcacctgcag gcccagcgga tggccgatac
     3301 cgtgcggcat aaacggtccg gtgagatcgt tttcgaccat cgcttcttca ctcatatcgg
     3361 tgatgatttg atgtttacgc agcaatttgg cgatgcgctg atggaactgg atgtggtaat
     3421 ccacatagct aacgccagct ttcatggtcg cgatcagcgc cagttgttca tcatttacgt
     3481 ctttcaccag ctgtgcgtaa tcgttgtcac tttttgccga ccaggtacgg gtcaggtcag
     3541 cggcatagcc gttatattcg gccccggcat ccagcaggaa gctgcgcatc tcttccggcg
     3601 cctgatgatc cagtttggtg taatgcagca ccgcagcgtg ttcgttaagc gccacaatgt
     3661 tgctgtaagg tacgtcggta tcacgatgac cagtcgcggt cagataggca atgttgatat
     3721 cgaactcgct catgccagaa cggaacgctt cttctgccgc acgatgaccg ttgaccgcca
     3781 ttttctgcgc ttcacgcata caggccagtt cgtactcggt tttgaaggag cgatagtaat
     3841 gcaggtagtc gataacccct ttcgggttga tgttgctggc ctcaataccc agttgcagcg
     3901 cacgctccgg caccggaccg atataaccga tattgccgcg cgcagcaggc agcaggctac
     3961 caatgccatc ggctttcggc agcgcgatca cttccacatc ttcagtccag aaggaggtcg
     4021 gcagcggttc gacgttgtgc cagtaatcaa ccggcagata aaaccacagt ttcggcttgt
     4081 tcacgccatc caccagcaac cagcagtttg gcacctgagt taccggcacc cacgctttga
     4141 attgcgggtt cactttaaac ggatagggat gatcgtcgag aaaaacattg aacagctcgc
     4201 cggagtgaat aagtaacgca tccagcttga agcgcgccag cgcatcgcga gtccgttctt
     4261 gtaaggtagc tatatgattt ttatagagcg aggccagtga ttccattttt tacccttctg
     4321 tttttttgac cttaagtctc cgcatcttag cacatcgttc atccagagcg tgatttctgc
     4381 cgagcgtgat cagatcggca tttctttaat cttttgtttg catattttta acacaaaata
     4441 cacacttcga ctcatctggt acgaccagat caccttgcgg attcaggaga ctgacatgct
     4501 ttacaaaggc gacaccctgt accttgactg gctggaagat ggcattgccg aactggtatt
     4561 tgatgcccca ggttcagtta ataaactcga cactgcgacc gtcgccagcc tcggcgaggc
     4621 catcggcgtg ctggaacagc aatcagatct aaaagggctg ctgctgcgtt cgaacaaagc
     4681 agcctttatc gtcggtgctg atatcaccga atttttgtcc ctgttcctcg ttcctgaaga
     4741 acagttaagc cagtggctgc attttgccaa tagcgtgttt aatcgcctgg aagatctgcc
     4801 ggtgccgacc attgctgccg tcaacggcta cgcgctgggt ggcggctgcg aatgcgtgct
     4861 ggcgaccgat tatcgtctgg cgacgccgga tctgcgcatc ggtctgccgg aaaccaaact
     4921 gggcatcatg ccgggctttg gcggttctgt acgtatgcca cgtatgctgg gcgctgacag
     4981 tgcgctggaa atcattgccg ccggtaaaga tgtcggcgcg gaccaggcgc tgaaaattgg
     5041 tctggtggat ggcgtagtca aagcagaaaa actgattgaa ggggcgatgg cgattttacg
     5101 ccaggccatt aacggcgacc tcgactggaa agcgaaacgt cagccgaagc tggaaccgct
     5161 aaaactgagc aagattgaag ctaccatgag cttcaccatc gctaaaggga tggtcgcaca
     5221 aacggcgggg aaacattatc cggcccccat caccgcagta aaaaccattg aagctgcggc
     5281 ccgttttggt cgtgaagaag ctctaaacct ggaaaacaaa agttttgtcc cgctggcgca
     5341 taccaacgaa gctcgcgcac tggtcggcat attccttaac gatcaatatg taaaaggcaa
     5401 agcgaagaaa ctcaccaaag acgttgaaac cccgaaacag gccgctgtgc tgggcgcagg
     5461 cattatgggc ggcggcatcg cttaccagtc tgcgtggaaa ggcgtgccgg ttgtcatgaa
     5521 agatatcaac gacaagtcgt taaccctcgg catgaccgaa gccgcgaaac tgctgaacaa
     5581 gcagcttgag cgcggcaaga tcgatggtct gaaactggct ggcgtgatct ctacaatcca
     5641 cccaacgctc gactacgccg ggtttgaccg cgtggatgtt gtggtagaag cggttgtcga
     5701 aaacccgaaa gtgaaaaaag ccgtactggc agaaaccgag cagaaagtac gcccggatac
     5761 cgtgctggcg tctaacactt caaccattcc tatcagcgaa ctggccaacg cgctggaacg
     5821 tccggaaaac ttctgcggga tgcacttctt taacccggtc caccgaatgc cgttggtgga
     5881 aattattcgc ggcgagaaaa gctccgacga aactattgcg aaagtcgtcg cctgggcgag
     5941 caagatgggt aaaacgccga ttgtggttaa cgattgcccc ggcttcttcg tcaaccgcgt
     6001 gctgttcccg tatttcgccg gtttcagcca gctgctgcgc gacggcgcgg atttccgcaa
     6061 gatcgacaaa gtgatggaaa aacagtttgg ctggccgatg ggtccggcat atctgctgga
     6121 tgtcgtgggc attgacaccg cgcatcacgc tcaggcggtg atggcagcag gcttcccgca
     6181 gcggatgcag aaagattatc gcgatgccat cgacgcgctg tttgatgcca accgctttgg
     6241 tcagaagaac ggcctcggtt tctggcgtta taaagaagac agcaaaggta agccgaagaa
     6301 agaagaagac gccgctgttg acgacctgct agtagaagtg agccagccga agcgcgattt
     6361 cagcgaagaa gagattatcg cccgcatgat gatcccgatg gtcaacgaag tggtgcgttg
     6421 tctggaagaa ggcattatcg ccactccggc agaagcggat atggcgctgg tctacggcct
     6481 gggcttccct ccgttccacg gcggcgcgtt ccgctggctg gacaccctcg gcagcgcgaa
     6541 atacctcgat atggcacagc aatatcagca cctcggcccg ctgtacgaag tgccggaagg
     6601 tctgcgtaat aaagcgcgtc ataacgaacc gtactatccc ccggttgagc cagcccgtcc
     6661 ggttggcgac ctgaaaacgg cttaaggagt cacaatggaa caggttgtca ttgtcgatgc
     6721 aattcgcacc ccgatgggcc gttcgaaggg cggtgctttt cgtaacgtgc gtgcagaaga
     6781 tctctccgct catttaatgc gtagcctgct ggcgcgtaac ccggcgctgg aagcggcggc
     6841 cctcgacgat atttactggg gttgtgtgca gcagacgctg gagcagggtt ttaatatcgc
     6901 ccgtaacgcg gcgctgctgg cagaagtgcc gcactctgtc ccggcggtta ctgtcaatcg
     6961 cttgtgtggt tcatccatgc aggcactgca tgacgcagca cgaatgatca tgaccggcga
     7021 tgcgcaggca tgtctggttg gcggcgtgga gcatatgggc catgtgccga tgagtcacgg
     7081 cgtcgatttt caccccggcc tgagccgcaa tgtcgccaaa gcggcgggca tgatgggctt
     7141 aacggcagaa atgctggcgc gtatgcacgg tatcagccgt gaaatgcagg atgcctttgc
     7201 cgcgcggtca cacgcccgcg cctgggccgc cacgcagtcg gccgcattta aaaatgaaat
     7261 catcccaact ggtggtcacg atgccgacgg cgtcctgaag cagtttaatt acgacgaagt
     7321 gattcgcccg gaaaccaccg tggaagccct cgccacgctg cgtccggcgt ttgatccagt
     7381 aaacggtacg gtaacggcgg gcacatcttc tgcactttcc gatggcgcag ctgccatgct
     7441 ggtgatgagt gaaagccgcg cccatgaatt aggtcttaag ccgcgcgctc gtgtgcgttc
     7501 gatggcggtc gttggttgtg acccatcgat tatgggttac ggcccggttc cggcctcgaa
     7561 gctggcgctg aaaaaagcgg ggctttctgt cagtgatatc ggcgtgtttg aaatgaatga
     7621 agcctttgcc gcgcagatcc tgccatgtat taaagatctg ggactaattg agcagattga
     7681 cgagaagatc aacctcaacg gtggcgcgat cgcgctgggt catccgctgg gttgttccgg
     7741 tgcgcgtatc agcaccacgc tgctgaatct gatggaacac aaagacgttc agtttggtct
     7801 ggcgacgatg tgtatcggtc tgggtcaggg tattgcgacg gtgtttgagc gggtttaacc
     7861 acttgccgga tgcggcgtga acgccttatc cggtctaccg atccggcacc aatgtaggcc
     7921 tgataagacg cgccagcgtc gcatcaggca tcgtgtgccg aatgccggat gcgacataga
     7981 tctaaatgcc actgaataaa gttttagttg ccgttcttcc cgcctgtcag gggcgggttt
     8041 ttttatatct cagataaatg caaacgcatc gccaaacagg cgatcttccc gcgcattacg
     8101 ctcactgcaa aacaggtcac gggcaatttt cgccatctca aaacgtccgg caatatagat
     8161 atcatgctct gccagcgtac cgtgatcctg caataccgcc gttaacacgg tgccagtacg
     8221 cccacgccag cccgcttccg gttgttctac caccggcacc acttgcagac caggatgctt
     8281 caacgaaagc gcctcaagct cgcagagatc atacagatgc tgctcttcac gcccgcccca
     8341 gtaaatggtg atatcacggt ttgggttacg cgccaacgct gtcagcaaaa tcgagcgggc
     8401 ataagagaac ccggtgccgc ccgcaatcaa aatcatcgga cgctcttcat catcgcgcag
     8461 ccacgcttct ccgtggggaa tatcgaccac gatttgatga tctttgagga tgcggtccat
     8521 gactgctttc gcgtaaaggt tgatttcaga agcgccaata tgcagctcga taaacccttt
     8581 ttcatccggc gtcgaagcca ttgagaacgg acgtttgtcg cgctcatcca tcactaccat
     8641 caaatactga ccagcacgaa aagaaaaggc cgcgtctggc acgatgcgga cacgatatac
     8701 ggtatccgtg atagcttcta ccgaggtcac tttacagctt aaggttgtca tgcgctttct
     8761 ctgtcggatc gataaatagg gcaaaacaaa cgcgcatcag gcgcttttac cgttgttaaa
     8821 aatagccagt tcatcccaga tggcgtcaat atgcgcgaca acatctggat cttttttgat
     8881 gggacgtccc cattcacgct gggtttcccc cggccattta ttcgtggcat ccagccccat
     8941 ttttgaaccc agcccggaga caggcgaggc aaaatccaga taatcaatag gcgtattttc
     9001 taccagaaca gtatcccgcg ccgggtccat acgggtggta atcgcccaaa tcacatcgtt
     9061 ccagtcgcgt gcgttgacgt catcatcgca aacgatcaca aatttagtgt acataaactg
     9121 gcgtaagaac gaccagacgc ccatcatgac gcgcttcgcg tgtccggcgt actgtttttt
     9181 gatcgtcact accgccagac gataagaaca gccttccggc ggcaggtaaa aatcgacaat
     9241 ttccgggaac tgtttttgca gaatcggtac aaacacttcg ttcaacgcca cgcccagtac
     9301 cgcgggctca tctggcggac gcccggtata ggtggaatgg taaatcgcat cttcacgctg
     9361 ggtaatatgc gtcacggtaa ataccgggaa actatcgact tcgttatagt aaccggtgtg
     9421 gtcgccatac ggcccttccg gcgccgtttc accttgttcg atataccctt ccagcacaat
     9481 ctccgcactg gcgggcactt caaggtcatt ggagatacac ttcaccactt cggtcttggt
     9541 accgcgcagc aatccggcaa acgcatactc tgaaagtgta tccggaacgg gggtgactgc
     9601 accgagaatc gtggcgggat cggcacccag cgccacagaa accgggaaac gttcgcccgg
     9661 atgcgccgca caccactcct gataatccag cgcgccgccg cgatgcgaca gccagcgcat
     9721 aatcagtttg tttttaccaa tcagctgctg gcgataaatg cccagattct gccgctcttt
     9781 atgcgggccg cgcgtcactg tcagccccca ggtaatcagc ggcgcggcat cttccggcca
     9841 gcaggtcata atgggaatgc gattgagatc gacgtcatcg ccagagacga ttttttgttg
     9901 gcagggcgca ccacgcagcc gctttgtcgg catgttcaat acttgcttaa actgcggcag
     9961 tttatcaaac aggtcgcgga aaccttttgg cggctccggc tctttcagaa acgccaataa
    10021 tttaccaact tcacgcagcg ccgaaacatc ttcctgcccc atgcccatcg ccacgcgctt
    10081 tggcgtaccg aacaggttgc acagcaccgg cattgagtag cctttagggt tttcgaacaa
    10141 cagcgcaggc ccaccggcac gcaaagtgcg gtcagcaatt tcagtgattt ccagatgcgg
    10201 atccaccggg agcgtgatac gttttagctc accctgctgt tcaagcagcg tcaagaagtc
    10261 gcgtaaatcg ttatatttca tggcgtccat tgtagcctct taatctacgc ccattatacg
    10321 gcgttcatct ttgcgatgct gtaaatttgt taaattagcg tgaactctga cggtataacg
    10381 caaaccgggg aatataatta acttagcgta aagcttttgc tatccttgcg ccccgattaa
    10441 acggataaga gtcattatgc aatcctggta tttactgtac tgcaagcgcg ggcaacttca
    10501 acgtgcccag gaacacctcg aaagacaggc tgtgaattgc ctggcaccga tgatcaccct
    10561 ggaaaaaatc gtgcgtggca aacgtactgc agtcagtgag ccattgttcc ccaactacct
    10621 gtttgtggaa tttgatccag aagtgattca taccacgact atcaacgcga cccgcggtgt
    10681 cagccacttc gtgcgctttg gcgcgtcgcc agcgatagtc ccatcggcgg tgattcatca
    10741 gctatcggta tataaaccga aagacattgt cgatccggca accccttatc cgggtgataa
    10801 ggtgattatt accgaaggcg cgttcgaagg ctttcaggcc attttcaccg aacccgatgg
    10861 tgaggctcgc tccatgctat tgcttaatct tattaataaa gagattaagc acagtgtgaa
    10921 gaacaccgag ttccgcaaac tctaaaacgc aatcccaaac agtgttttga cattagcatc
    10981 cgtggtggca gccagccatg cggcatcttc tccacgccag tgcgcaatac gttgcaaaat
    11041 atggggcaga tgggctggct cgttgcgccg ggatgatggc tttggcgtga gatcgcgagg
    11101 gagcagatac ggcgcatcag tttcgatcag taatttttcc gccggaatca acggcaaaag
    11161 ttcccgcagc tccagtccgc gtcgttcatc gcaaacccaa ccggtaatgc cgatataaat
    11221 tccatgcgcc acgcacgcct gcatctcttc gcgtgtgccg gtaaagcaat gaagaaccgc
    11281 accaggcagt ttatccagcc acggctccag caatgtcata aaccgctcgt gggcatcgcg
    11341 acagtgcata aataccggca tgtttaaatc tgcggcaatg cgtagctggg caacaaaagc
    11401 gcgttcctgc tcttccggcg tcgaaaagtt gcggttaaag tcgagaccac attcaccaat
    11461 cgccaccact tctggctgcg cggccagctc aataatcgct tcttcagtcg cagcttgcca
    11521 ctggctgctg tcgtgaggat gtacgcccgc cgttgaccaa caggatgaat actgacgcgc
    11581 cagcttttgc gcctgctggc tttcacgcag gttagtgccg gtgatgagta gcccattaac
    11641 tcccgcgtca aaagcgcgcg ctacaacatc atcacggtct ttcgcaaatt gcgaactggt
    11701 caaattaacg ccgatatcaa acatcctgta ctccatatga caaccgccct gacgggcggt
    11761 tgaatttatt cttcagtttt ttcgctttct gcttcagcgt cgttttcctc ttcccgattt
    11821 cgccctttac caacgtaaaa gcgtgagaag aagacaccga tttcaaacag acagtacatc
    11881 gggatcgcca acagcgtttg cgagaagaca tccggcggcg tcagcaacat cccgacaacg
    11941 aatgcaccaa ccagcacata cggacgtttt ttgcgtaagt cttctggcga ggtaatcccc
    12001 atccagcaca gcagcacaat tgccaccggc acttcaaagg agacaccaaa cgccataaac
    12061 agcgccataa cgaagcttaa atagctggcg atgtcggtgg atacctgcac cccttccggc
    12121 gcggtattgg caaggaagcc aaatgccagc ggaaagacca caaagtaggc gaacgccatg
    12181 ccgatataaa acagcagaga gctggaaacc agcagcggca ccaccaggcg acgttcatgc
    12241 ttatacagcg ctggggcgat aaatgcccac acctgataga gaatcaccgg cgctgacaga
    12301 atcagcgaca ccataaaggt cagcttgatc ggcgtaaaga acggcgaggc cacgtcggtg
    12361 gcgatcatcg ttgaaccttg cggcaactgc ttgatcaatg gcgcggatac caggtgatag
    12421 atgtcattgg cgaaatagac cagacacagg aatatcacga tcaccgcgat aatgcagttc
    12481 agcagacgct tacgcagctc aatcagatgc gtgataagcg gttgagtatc ttctacagac
    12541 atgtttacgg tttatcactc gacgaagggg aaggtgcagc ggttttcggt tcagcgtccg
    12601 cagcaggttt taccaccggc tctggcgtgg tttctggctt ctgttccggc gaactggcct
    12661 gcgtttgtgc agcggcaggc gttacgccct catgcgcagc ttcattatct ttcaccaccg
    12721 ggttatggat ggtgtgcgct tcatcgctcg ccttttcagg atcgtttgca acgtaggaac
    12781 gcttcatcga ctccgcggcc tggcgtagtt catccatcga cgctttcagt tcgggcgtca
    12841 ggttagtgag gctcgccttt tcaacctttt tcagactgtc ctgaaactcc tggagtttta
    12901 actcctgggt cagttcgttc tgcaccgttg tcgccagtga acgcaacgcg cggatccagc
    12961 ccgctaccgt ttttaccgcc acaggcagtc gttgcggccc cagaacgacg aggccgatga
    13021 tgaacaccaa tagcagttcg ctaaaaccga tatcaaacac ggattacacc tgctctttat
    13081 cgtggcgctt cgcgtcttct gttttagcct gttcctgatt cgtatccgcc tgcttatcgg
    13141 cgatagtttt cgcagtaaaa tcagcatcct gactggtttt atcctgcttt ggttcatcat
    13201 cgctcattgc ttttttaaag cctttgatcg acgcaccaag atcggaaccg atggagccga
    13261 gctttttggt gccaaaaagc agtacaacga tgacggcaat aatcaataac tgccaaatac
    13321 tgataccacc catacatgtt cctctgtggt agatgatgat taaacaaagc cgcattatac
    13381 gttacacggc ccgccttgag cgatgaaaaa atcagcgtgt tttgcgccaa ccgacaaacc
    13441 aggcgatcag accacctgcc attaaccagc cgggcatcag cccccattca ggtcggctga
    13501 ccaacaagaa tgtgccactt aatactaacg tagcgccaat tccgagaaaa taacgcgatt
    13561 gtccctgacg tacatgattt gactgaagct cgcgggcaat cttatcaaca ctgtgctgta
    13621 aatacttgcc ctggcgcaaa ctgtcgtaaa ccagttcagg cagttctggc attttttcga
    13681 cccagaacgg cgctttttct ttaaatgctc tcaccagcgc aggaataccg acctgatctt
    13741 taatccacga ctccaggaaa ggcttcgccg ttttccataa atcgagttgc ggataaagct
    13801 ggcgtcctac cccttcgacg tagagcaggg ttttctggag taacaccagt tgcggctgca
    13861 cttccatatt gaagcgacgc gccgtattaa acagatttaa cagtacatgt ccaaacgaaa
    13921 tttcggccag cggtttctca aagataggtt cacagaccgt acgaatagca aattcgaact
    13981 cttcaacgtt ggtatctggt ggcacccagc cagaatcgac gtgtagctct gccactttgc
    14041 gatagtcgcg attaaagaag gcgataaagt tttctgccag atagcgttta tcttctttgt
    14101 ttagcgagcc aacaatcccg caatcaatgc cgatatattt cgggttttcc gggtgttcat
    14161 agcttacgaa gatgttgcca gggtgcatat cggcatggaa aaagctgtcg cgaaagacct
    14221 gagtgaagaa cacctgcacg ccgcgttccg ccagcaattt catgttggtg ccgtttttct
    14281 ccagcgccgc aacatcagac accggaatgc cgtaaatgcg ctccatcacc atcatccctt
    14341 cactacaata gtcagggtaa acttccggga tgtagagcat cgggctgtct tcaaaattgc
    14401 gccgaagctg aatggcgttg gcagattccc gcagcaaatt cagttcatca atcaatgtct
    14461 tttcgtactc gcgcaccact tcggttgggc gcagacggcg accatccggc agcaaacgcg
    14521 gcacccagcg agccagacgg tagataagtt tcagatccgc tttaataacc ggcaaaatat
    14581 ccgggcggat gactttaatc accacctctt taccattcga tttcaatcgc gcggtatgaa
    14641 cctgggcgat agaagcagaa gccagcggct tgatttcaaa atcgtcaaac cacgcttcta
    14701 ccggcaagcc gcccattgca gcttcaatct gctgcttcgc cagcttgcca tcaaacggag
    14761 caactttgtc ctgcaataac gccagctgat cggcaatatg cggcggaaaa agatcgcggc
    14821 gggttgataa catttgcccg aacttgatcc ataccggccc cagttcttgc agggccagtc
    14881 gtagtcgctc acctaaaggt ttgtctttat gccgatttgg catccagaat aatgagtatc
    14941 gccatagccg tagcggcagg gtgatacgca ttttggggat cagttcatca agtccgtagc
    15001 ttaaaaaagt gcgaatgatg aaatataggc gccgtacttc acctggcgtc atttagcctc
    15061 cagtttttcc agccgtttgg tcagggcatc aacagcacgc tcgacggcag ccgtctcctc
    15121 cgcaaaccag gccacttcaa gcggaccggg tgccatacgc cactcttcag taatggcttc
    15181 cgccacataa cgttgctggc gcttaatgcc gtgatgcagg aactttgcgc ctccgcgcat
    15241 ggctttgctg attccttcag cggcgatatc accgatataa ggggccagca gttccgcagg
    15301 gtcgaactct gccagatctg ccagcgcaac gaagttttgc accacctgaa tatcgccctg
    15361 cacttccagc tcaccactgc gaatcagggc ggcaagctgc tggcgatcgc gaagtttcgg
    15421 caacacactg gcgtaggcga taacggtgca gtcagcatcg cctgcccatt cgcccagtac
    15481 atcaacctgg cgttcgctga acaccagaat caatgacgtc gaaaagcctt ttacctccac
    15541 gcgcagcact ttgcccaaca gacgcgagcg ggccgttttc agcgcgggtg agcgatacag
    15601 gaaggtgttg agcagacttt caattcctgc cgtcactaaa ggtttaaaag gcatttccgg
    15661 tctcctgtca gaacttataa ccacgatgca gcgccacaac ccctgccgtc agattgtagt
    15721 agtcgacact ttcgaatccg gcatcctgca tcatggcttt cagggtatcc tgatcgggat
    15781 gcatacggat ggattctgcc agataacggt agctgtcggc gtcgttcgcg accagtgagc
    15841 caatacgcgg cagcacatgg aaggagtatg catcataggc tttgctcagc ggctcgataa
    15901 ttggcttcga gaactcaagc accagcaggc ggccgccggg tttcagcacg cgatacattg
    15961 aacgcagtgc tttatctttg tcggtgacgt tacgcagacc aaacgaaatg gtgatgcaat
    16021 caaaggtgtt atccgggaac ggcagcgcct cagcgttcgc ctgaacatac tcaacgttgc
    16081 caatcacacc gatattacgc agcttctcgc ggcccatttt gagcatggat tcattgatat
    16141 cagcaaggac cactttgcca gtttctccga ccaggcggga gaatttcgct gtcaggtcgc
    16201 cggtgccacc agccagatcc agcacggtct gcccacggcg tacgccgctg caatcaatcg
    16261 tgaatcgctt ccacaaacga tgaataccaa atgacatcaa atcattcatg acatcgtatt
    16321 ttgatgccac ggaatggaaa acgtgggcga ccatatccgc tttttgttcc ttcgcgacgg
    16381 tctgaaaacc aaagtgcgtc gtttcttgtg acttatccac catctcaatg cctgctcatc
    16441 aaaaaattgt tccagaagtg taacagattg ggcgtcgatg ccctagattt ctacccggct
    16501 taactactcc caatgggcta gcgcgactgc tgattatatt catcatcgcg ttgataagct
    16561 tcatcattcg gctgctccgg aaccgaccga agtcgatact cttcatcctg gctcaccgcc
    16621 tgttcagcca aatccgaatt aatctcgcgt ttaatttcta ctcctaaacc gcgaaacgct
    16681 tctgcctgcg ccagcacatt tccgcgccct gaagagagtt ttttcattgc ctgccgataa
    16741 ttatcctgcg ctttgtcgag actttgacca atcgcggaca tgtcatcgat gaacaaacgc
    16801 atcttgtcgt acagcttgct ggcacgatcg gcgatttgct gggcgttgcg gctttgatgc
    16861 tcataacgcc acaggttggc gatagtgcgc agcgccacca gcagcgtagt cgggctaacc
    16921 agcatgatat tgtttttcaa cgcttcggtg atcagctccg gctggcggtc aagcgccagt
    16981 aaaaaagcgg gttcaacggg aataaacatc agcacgtaat ccagagttcg cagccccggc
    17041 agctgttgat aatctttgcg tcccagcaaa cggatatggt tacgcaccga cgcgatatgt
    17101 tcctgtagcg cgctttcgcg ggtgtagtcg tcttcggcgt taaaatagcg ttcataggcg
    17161 accagcgtca ttttggcgtc gatcaccaca tcttttccct gcggcaggcg cacgatgaca
    17221 tccggctgca tccgcgagcg ggcgtcattt tcgatgctga cctgggtttc atattcatac
    17281 ccttcacgca gaccggaagc ctccagcacc cgcgtcaata ctacctcgcc ccagttgccc
    17341 tgggttttat tgtcgccttt cagcgcgcgc gtcaggttga tcgcttcctg ggccatttgc
    17401 gcgttgagtt gctggagatt gcgaatttcg tgggtcaggg tatggcgttc ttgtgcttct
    17461 ttaccgaagc tgtcctgaac ctgacggcgg aaaccgtcca gttgttcacg tagcggcgac
    17521 aacaggctgt tcagactctg acggttttgc tcatcaaccc ggcgattgct gtgctcaaaa
    17581 atacggttgg cgaggttttc aaactgctca ctgaggcgct gctcgctgtt aatcatctgg
    17641 cgaattttat cgtcagcatg ttgctgtgcg gcttccatcc gcgtggttac ttcacgcaga
    17701 tcggcctcca gagaggtgtt aatactttgc aggctgcgca cttcgttatt gagtaactcg
    17761 cactctgcac gccagtgctc gctttgggta atttgttgtt ttgccgcgct taactccgcg
    17821 accatctctt cacgttcagc taattgctcg gctttttgct gcgcatgttg ataactggca
    17881 aacagccagc caattgccac acccaccaac gcaataactg cgtaaaccat gattgagaaa
    17941 tccacaacgc ctcctgacgc aacaacttac ccgcacaaaa tagaattgtg ctgtacaaac
    18001 gtccagttgg aaaggcattt cctgcgaggc gctacgcaaa aatacaaaag gccgaacgcg
    18061 tcggccttca gacaggagaa gagaattaca gcagacgacg cgccgcttcc accacgattt
    18121 tcaccgcatg gctttcggtt tgtttcatcg tctcagcatt cgggatctct tgctgggtgc
    18181 ggttaacgat aacacccgct accataccgg cacgcaggcc ctgacttgca cacatggtca
    18241 gcagggttgc agattccatt tcatagttca ttacgcccat cgcctgccac tcttccatag
    18301 aacctttaaa gtgacgaact acgcgaccag agtaagtatc gtaacgttcc tgacctgggt
    18361 agaaggtatc agaagaagct gtcacgccaa cgtgagttgt cgctccaatg gatttcgcag
    18421 cttcaaccag cgcagtcgta cattcgaaat cagcgacagc cgggaattcc agcggtgcga
    18481 agtgcaggct cgcgccatcc agacggacag acgccgtggt aaccaggaca tcacccacat
    18541 taatatgcgg ctgaatagcg cccgttgtac cgatacgcag gaaggtgcga atgcccagct
    18601 gtgccagctc ttcaacagca atagaggtag acgggccgcc gataccggta gagcagacga
    18661 taacaggttt accatccagc tctgcacgcc aggtagtgaa ttcgcggtga gatgccagct
    18721 taaccggctt atccatcagc gcggcgatct tttccacacg atccgggtcg ccagggacga
    18781 tggcaagcgt agccccttgt aaatcgtttt tagtgaggcc gagatgaaaa acatcagact
    18841 tggacatata caactcctct gtgaatcggt ttagtcagaa gaagcaaaaa gacactttac
    18901 cgaagggttt aacatttttt cgtgatactc atcaccatga cgcaaatgcg ttgcataaat
    18961 ttacccacca aaagtgattc aaatcacata aatcactctt cattctgcag gactgcataa
    19021 aagatgagcc gattcaggca atgtgatttg ttgcatgctg actatagtta acattgcgca
    19081 acatttttat aagggtacgg agaataccat ggcaacaaca caacaatctg gatttgcacc
    19141 tgctgcatcg cctctcgctt cgaccatcgt tcagaccccg gacgacgcga ttgtggcggg
    19201 cttcacctct atcccttcac aaggggataa catgcctgct taccatgcca gaccaaagca
    19261 aagcgatggc ccactgccag tggtcattgt agtgcaggaa atttttggcg tgcatgaaca
    19321 tatccgcgac atttgtcgcc gtctggcgct ggaggggtat ctggctatcg cacctgaact
    19381 ttacttccgc gaaggcgatc cgaatgattt tgccgatatc cccacgttgc ttagcggtct
    19441 ggtagcaaaa gtgcctgact cgcaggtgct ggccgatctc gatcatgtcg ccagttgggc
    19501 ttcccgcaac ggcggcgatg ttcatcgttt aatgatcacc ggattctgct ggggtggacg
    19561 tatcacctgg ctgtatgccg cgcataatcc acagctaaaa gccgcagtgg cgtggtacgg
    19621 caaactgacg ggcgacaagt cgctgaattc accgaaacaa cctgttgata tcgcaaccga
    19681 tcttaacgcg ccggttctcg gcttatatgg cagtcaggat aacagcattc cgcaggagag
    19741 cgttgaaacc atgcgccagg cgctgcgggc tgctaatgcg aaagcagaga ttatcgtgta
    19801 cccggatgcc gggcatgcat tcaacgctga ttatcgcccg agctatcatg ccgaatctgc
    19861 gaaagatggc tggcagcgta tgctggaatg gtttaagcag tatggtggga agaagtcgct
    19921 gtaatgagaa aagaccgggt ggtattacca cccggtttgg attttacccc cgacgcaaat
    19981 tctgcgccgc ctgtaccatg ttcgccagcg cggcgcgggt ttctggccag ccgcgcgttt
    20041 tcagaccaca gtccgggtta acccacaggc gctctgccgg aatgcgttgt gctgctttct
    20101 tcagcaaggc ttcaatccat tctacgctcg gtacgtttgg cgagtgaatg tcatagacgc
    20161 ctggaccgat ttcattcgga taatcaaact cttcaaacga ctccagcaac tccatgtcgg
    20221 aacgcgaggt ttcgatggtg atgacgtctg catccagtgc cgcaatcgaa tccatgatgt
    20281 cgttgaactc gcaataacac atgtgagtgt ggatttgtgt gtcatctttc gccacggcgg
    20341 cgttgatacg gaaggcctcc acgccccact ggagatatgc atcccagtcg ctacgacgca
    20401 gcggtaaacc ttcgcgcagc gccggttcat caatctggat gatgccaatt cctgcggctt
    20461 ccaggtccgc cacttcatca cgcagcgcca gcgcaatttg tttggcgatg gtttcacggc
    20521 tgacatcttc acgcgggaac gaccagcaga gaatggtcac cgggccagtc agcatccctt
    20581 tcaccggttt atcagtcaga gattgcgcat acttcgccca ctccacggta atcggtgccg
    20641 ggcggctaat atcaccaatg acaatcggtg gcttcacgca gcgggaacca tagctctgta
    20701 cccaaccgtt ttgcgtaaag acaaacccgt cgaggtgctc gccaaaatat tccaccatgt
    20761 cattacgctc ggcctcgcca tgtaccagca catccagtcc caaacgttcc tgttcaacaa
    20821 tggcctgcct gatatgttcc gcaatgcccg tgcggtagtt attggcgtcg agattgccct
    20881 ttttgaaatc cagacgcagg gtacgaattt ccgtggtttg cgggaaggaa ccaatcgtgg
    20941 tggtcggcca cgctggcagt ttaaaacgcg cacgctgggc ttcggcacgc acttcataga
    21001 cattcgcacg ctggctgtcc tgggcggtga tcgccgccag acgcttttct actgccgggt
    21061 tatgtacacg agtagagtga cgacgcgcct gaattggggc gctccactct gccagagctg
    21121 ccgtgtcacc actgttcagc gcatcgcgca gcagtgccag ttcatggcat ttttgtaggg
    21181 cgaaggcaaa ccagcttttc acttctgcat caagacgcgt ttccacgctc aggtcgatgg
    21241 ggctgtgcag caacgagcag gatgatgcca cccacaaatc acgtttgccg acaatgtcct
    21301 taatttgcgc atatttctca gtaagatcgg cgcgccagac gttacgacca ttgatcagac
    21361 ccgcagacag cagccagtca gaaggcaggc gcttgtgcag ttcaacaacg tcatctttac
    21421 catgtacaag gtccacatgc agcccctgaa caggcagcgc agtaatcgtg tcgagatttg
    21481 gcgttacgcc ttcaaaatag gtggtcagca gcagtttcac ctgtccctgg agcgcgtcgt
    21541 aagcgggttt gtatgcgtcc agccacgcct gtggtagttc cagtaccagc gcgggttcat
    21601 caatctgtac ccactcgatg ccgcgtttcg ccagttctgc cagcacttgc tgataaaccg
    21661 gcagaatgtc gttcagcagg ctaaggcggt caaactgttc acctttcact ttccccagcc
    21721 acagccaggt aatcggcccc agcagcacag gtttcacctt gtggcccagc gccagcgcct
    21781 cgtccacttc gtccagcagc tgcgtccagg tcagtttgaa ctgttggcct ttaacgaact
    21841 ccggcaccat gtagtgatag ttggtgttaa accatttggt catttccgct gccgccgcag
    21901 gttcgccagt cggcgcacgt ccacgaccaa tacggaacag ggtgtcgata tctaccgaac
    21961 catctttgtt ctgatgacgc gccggaacgt tacccagcag cagactggtg gtcagtacat
    22021 gatcgtacca ggcaaaatcg cccaccggca gcaggtcgat acccgcttgc ttttgttgat
    22081 cccagtgacg agcacgcaat tcacgcccta ccgccagcag ttcttcacgc gtggagttcc
    22141 ccgcccaata actctcttgc gcttttttca gctcgcgacg caggccaacg cgagggaaac
    22201 cgagggtgtg attcaatatt gtcatttttc ttcctctaat tatatgtaaa tcctatggat
    22261 tttgaattta gggaaggcgg caagtttatt catccccggg agcttactga agtaagtgac
    22321 cggggtggag aaacgcagcc aacgcaccat aaatttaaaa gacgaaggat tttagccgtc
    22381 cagatgttta cacatccata attggcggtt actgtatatt cctcaagcgc aaattattca
    22441 tgccgaagtg aaggactttc atgatcgaag taaaacacct gaaaacgcta caagcgttgc
    22501 ggaactgcgg ctctctcgca gccgctgcgg cgacgttgca tcagacgcaa tccgccctgt
    22561 ctcaccagtt tagcgatctg gaacaacgcc ttggcttccg gctctttgtg cgtaagagcc
    22621 agccgctacg ctttacaccg cagggagaaa tcctgttgca actggcaaac caggtgctgc
    22681 cgcaaatcag ccaggcactg caagcctgca atgaaccgca gcagacgcgt ctgcgcattg
    22741 ccattgagtg ccatagctgt attcagtggc tgacgcctgc gttagaaaat ttccataaga
    22801 actggccgca ggtagagatg gattttaaat cgggcgtgac gtttgacccg cagcccgcgt
    22861 tgcaacaggg cgagctggat ctggtgatga cctccgatat cctgccgcgc agtggcctgc
    22921 attattcgcc gatgttcgat tatgaagtac gcctggtgtt agcacctgac catccattgg
    22981 cggcaaaaac gcgtattaca ccggaagatc tcgccagcga gacgctgttg atttatccgg
    23041 tgcagcgtag ccgactggat gtctggcggc attttcttca gccggcaggc gtaagcccgt
    23101 cactgaaaag cgtcgataac accttattgt tgattcagat ggttgccgcg cggatgggta
    23161 ttgccgccct gccgcattgg gtagtagaga gttttgagcg ccagggtctg gtggtgacca
    23221 aaaccctggg cgaaggcttg tggagccgac tgtacgccgc cgtgcgcgat ggcgagcagc
    23281 gtcagccagt tacggaagcg tttattcgct cagcgcgcaa tcacgcctgc gatcatctgc
    23341 cgtttgtgaa gagcgcggag cgacccactt acgatgcacc cacagtgagg ccaggatcac
    23401 cagcgcgcct gtaataaacg ttggccagtg cggctgttgg tgccagatag ccaggtttac
    23461 cagcagccct gccggaacgt gcatattatt cataattccc agcgttccgg cgtccacctg
    23521 cgtcgcgccg tagttccaca taaagtagcc aatcccggaa gccaccacgc caagaaacac
    23581 cagaatgccc cattgcaacg tggtttgcgg cattttctgc gcatttccca gcaagaacca
    23641 tgcaattacc gccaccagga acgcgccaag atagaaccac gcaaaggcgt tatgctgtgg
    23701 catcgggcga gtttccatca ggcgtttgta acccaccatg ccgatggcaa aagtgatatt
    23761 ggagagttgc accagcaaca agccagtcca gaaatggtcg gtgacctgat catagcgaat
    23821 aatcccggca ccaatcaccg ccaacaaggc actaaaagca tatccccagc gcagacggcg
    23881 cttactcatg atgtcataaa tcagcgtgat gtagagcggc gtcagtacgg tgaacagcag
    23941 cagttcggaa accgtcaggt agagataagc gcggaaactc agcatataca tcacgccgag
    24001 ctgcatcgcg cccaccagca tatacaggcc gactgttttc aggctattgc cacgggtacg
    24061 cagaaacggc agaaaaacga gtgccgccag gccaacgcgc accagcaccg caaaatagct
    24121 atcgacgtgc cccgcaaggt actcgccata aaaactaaag gagaaggccc acaaaatcgt
    24181 ggtgatgata agtagcgcca caatggaaaa ctctttgatt aacgagtatc cattgtagcg
    24241 aagtatcagg ttgacaactg accaaataaa gaacgattac gataaataga gtttacgcag
    24301 ataatgcggc accgcgtcgt cggcattagt accaatcact tccagctcgg gatgaaggtc
    24361 tttcagacgc tggtgcgcac tgcccataat gcagcctttc cccgccatcg acagcatttc
    24421 ggcatcgttc atcccgtcac caaacgcaat acaatccttc aggctgtagc ccagtttctt
    24481 cgccaccgct tccagcgcat ggccttttga aacgccgccc gccatcactt ccagacaggt
    24541 taaggtagag aaactgacgt tgacgcgatc gccccaacga gcgttaatcg cctgctccag
    24601 cggcagcagt tgttcatggg aatcgcaggt gaagaacact ttgctgacac cttccggctc
    24661 cagtaatcca ggctcataca gcgcatattt gaacaccgct tctttaaaaa agcgcatctc
    24721 ttccgggcga tggcgattca taaaccattc gtcgtcgcga taaacgttag taatgatgtc
    24781 cggattgtcg ttgactacgc caaacagatc gctggcaatg tcgcgatcca ggttatgagc
    24841 aaaaatcaga ttaccatcca gatcgtgaac gcgcgcacca ttggaggtaa tcatgtaaga
    24901 cttaatctcc agattatcgc gaatttgccc cacatcaacg tggtgacgac cggtcgcaaa
    24961 cacaaagttg atgccgcgcg cggtgagcag cttcagggtt tctttggcgt aaggggataa
    25021 cgtatggtcg ggagaaagta acgtgccatc taaatcagac gcaacaacct ggtacataag
    25081 aaatttaaac ctctgtagag cggtttccgc tgggtgagtt atgcctgttg aaaaaatcaa
    25141 cgatggcgtg gagcgcaact gagcgcattg cgtccttttc aaaaaggatc tcatggtacg
    25201 cacctttaat taccagcggt cgtccccctt cgacaggatg gcccgcggcg gtgcggagtt
    25261 cacaaaaacg gtcatgcatg cggttatcca ccacgcgttc ctcttcagcc tgcaagagaa
    25321 gcgttggcgt ggcgtcatca cccgcaccgg ctaacacctg ttcgccagcc agaatacttt
    25381 cgcgtaccca atggtaggtc ggcccaccga cgcgaatcgt tgggtcatca gcatagaagc
    25441 gtaagttacg tcgatatcgc tgtctgctgt gggtcagtac gttgatagca aacggcaacg
    25501 cgcgccagcg cccggtgcct attgcatagc catcacggaa acgtggatgc gcttcggccc
    25561 agttgaggat ctgccgtgcc ataaatgacg gcatacgaat cacgatccca aacattggcg
    25621 cagttagcgc aatggcgtca catacacctg gatggcgttg cagaaataat gtggagatcg
    25681 caccgcccat cgaatgtgcc agtatatagc gtttacgcca cggaccgggc tggacctcct
    25741 gttgccagaa tgccgccaga tcatcaacat aatcattaaa gcgattaaca tgcccgagat
    25801 gcggatcggc taacaggcga ccggaacgtc cctgcccgcg atggtcgatg attaagacat
    25861 caaaccccaa atggaacaaa tcgtaggcca gttccgcata ttttacgtag ctctcaatac
    25921 gccccgggca gatgactacc acccggtcat ggtgctgtgc gcgaaaacgg acaaagcgca
    25981 ccggaatgtc atccacacca gtaaactctg cttcatcacg ctgacgccag aaatcagtca
    26041 gcggtcccat ggtaaaagca gcaaacgcgt tttctcttgt ttcccagtct ttttgctgct
    26101 gaaacatcgg gtaatctgcc tcttaaacca cgtaaaatcg ttttttttag cgtgcctgac
    26161 acaacgctgc gacagtagcg tattgtggca caaaaataga cacaccggga gttcatcatg
    26221 accttagaat ggtggtttgc ctacctgctg acatcgatca ttttaagcct gtcgccaggc
    26281 tctggtgcaa tcaacactat gaccacctcg ctcaaccacg gttatcgcgg cgcggtggcg
    26341 tctattgctg ggcttcagac cggactggcg attcatattg tgctggttgg cgtggggttg
    26401 gggacgctat tttcccgctc agtgattgcg tttgaagtgt tgaagtgggc aggcgcggct
    26461 tacttgattt ggctgggaat acagcaatgg cgcgccgctg gcgcaattga tcttaaatcg
    26521 ctggcctcta ctcaatcgcg tcgacatttg ttccagcgcg cagtttttgt gaatctcacc
    26581 aatcccaaaa gtattgtgtt tctggccgcg ctatttccgc aattcatcat gccgcaacag
    26641 ccgcaactga tgcagtatat cgtgctcggc gtcaccacta ttgtggtcga tattattgtg
    26701 atgatcggtt acgccaccct tgctcaacgg attgctctat ggattaaagg accaaagcag
    26761 atgaaggcgc tgaataagat tttcggctcg ttgtttatgc tggtgggagc gctgttagca
    26821 tcggcgaggc atgcgtgaaa aataatgtcg gatgcggcgt aaacgcctta tccgacttac
    26881 tctgaagacg cgtctggcat caccgcgaaa taatcaaatg aatgccaaat ccggcaaata
    26941 acgccccggc aaaaccatca atccacttcg ccagacgttg ataaccacgg cgcatttgcg
    27001 gcagggcaaa caggctggca acgacggtaa accacgccag tgtttcgaca atgatcagcg
    27061 caaaaatgcc ccagcgcgcg gtagtgccaa cgttatcacc gacaaacaat gagaacaccg
    27121 agccaaagta gataatcgct ttcggattag cgagattggt cagtaaacct ttcaggaaac
    27181 tgcgcccact tttcgccagc tcgacctgtg gcgcaggtgc agaaaccgcc tcttttttca
    27241 gcgcgccgcg cagcatctgg taacccatcc agcagagata caggccaccg ccaaccataa
    27301 tcagcgtatg cagccaggcc attttttcga taatcaaatg caggccaagc aacgcaatcc
    27361 ctgcccaaac cataacgccg caggtaatac ccagcacgcc catcatcgct tctttacggg
    27421 aacgactgac agcggtctga gagacaaaaa agaaatcggg accgggactc ataagcgcca
    27481 caatgtgcac catggcgacg gtgagaaata acatcaacat acatttgact cgcgggggaa
    27541 acgtttacgg agtcttcatc ctggcacttt tttatgctgc tgactactct tcgtcgtcgc
    27601 catcgacatg cgcacgaatc agcgccataa acggtttacc aaagcgttcc agcttgcgca
    27661 tcccaacgcc gttaacgctg agcatttcgc tggcggtgat cggcatctgt tcagccatct
    27721 caatcaaggt tgcgtcgtta aacaccacgt acggcgggac attactttca tcggcaatcg
    27781 atttacgcag tttgcgtaat ttggcgaaca gtttgcgatc atagttgccg ccgaacgatt
    27841 tctgcatcgc tttcggtttg agcgccacga tacgcggcac ggcaagttgc aaagaggatt
    27901 cgccgcgcag caccgggcgc gcggcctctg tcagttgtag ggcagaatgc tgggcaatat
    27961 tttgcgtcac caggccgagg tgaatcagct ggcggatcac gctcacccaa tgttcatggc
    28021 ttttatcacg gcccatgcca tagactttca gtttgtcatg accatagtcg cggatacgct
    28081 ggttattagc accacgaatc acttccacca cataacccat cccaaaccgc tgattcacac
    28141 gaccaatggt ggaaagggca atctgagcat cggttgaacc gtcgtactgt ttcggcggat
    28201 cgaggcagat atcgcagttc ccgcacggct cctgacgccc ttcgccaaaa tagttcagca
    28261 ataccagacg acggcaagtt tgcgcttcgg caaacgcgcc catcgcattg agtttgtggc
    28321 gctcgatatc ctgcaactgc ccctgcggct tctcttccag acaacggcgc agccacgcca
    28381 tatcagccgg atcgtaaaac agcatcgctt ccgcaggcag gccatcacgc ccggcgcgac
    28441 cggtttcctg ataataggat tcgatattgc gcggaatatc aaagtggacc acgaagcgaa
    28501 cgtttggttt attgatgccc atgccgaacg ccaccgtcgc caccacaatt tgcaggtcat
    28561 cgcgctggaa tttctcctgc acgtcggcgc ggacattatt ttccagcccg gcatgatagg
    28621 ccgccgcgct aatacccttg ctttgcaggc gcgcagcggt gtcttctact ttcgcccggc
    28681 tgttgcagta gataatgccg gacttaccgc gctgttcctg cacgtagcgc atcaactgat
    28741 cgagcggttt gaatttctcc atcagcatgt agcgaatatt cggacggtca aaactgctga
    28801 tttgaatcag cggatcgttc agccccagca ggcgcacgat atcctggcgc gtggtgtcgt
    28861 cggctgtggc ggtcagcgcc ataaacggca gcgtcgggaa ccgctggcgc aactgaccga
    28921 gcgcggcata ttccgggcgg aaatcgtggc cccattggga gatacagtgc gcttcatcaa
    28981 cggctaataa caccggattc cagtgcgcca gatgctcaag aaagttatcc agcatcaggc
    29041 gttccggggc gatataaagc agacgaattt gcccggtgcg gcagcctgtc atcacttcaa
    29101 gttgctgttc gcgggtttgc gtcgagttaa ggcacgccgc cgccacgccg ttggcttgca
    29161 gttgatccac ctgatctttc atcaacgaaa tcagcggtga aacaaccacg gtaaggccgt
    29221 ttagcaataa ggcaggaatt tgatagcaaa gggattttcc gccaccggtg ggcatgacga
    29281 ccaggcaatc gcgaccggaa agcacagtgt cgataatttc ttcctggccc gggcgaaact
    29341 gttggtagcc aaaggtttct tgtaaaacct gtttagctcc ggactccaga ttcaacactt
    29401 ccgcctgcgc cacattcacc ccgattgcct gaaataaaaa caggcgctat tttcagcgcc
    29461 tgcgcgagaa actgcaatgc ttttagtctc tgagagggta atcctggatg ctggattcca
    29521 gatttgccaa ccactcaacc gttcatcaaa acaaatcgtt tagcataacc cccacaccga
    29581 cacgggtctg gttgaagtta tagtcgatga gcgattcgcc atagccgctg taaacctgag
    29641 tataaaggcg cacatgtttg gtgatcgggt aacttaagcc taactccgcg ccgccgtagc
    29701 cggtgttcca gttgtactgt cctttcgcac tgagcaccgc atcaccgagg tgatagccga
    29761 ttttaagctg gtagtaaccc atatatttgg tgatatccgg gttatcgtca gtattaccca
    29821 ccacatacca cggcttcact tctaccagcc agttaccgtt ttctgccatc aggcgagtat
    29881 aaaggcggtt ccagctgcgg gaggtcgggt ctgaacgccc gttagagtcg tggttatacc
    29941 ccatctctac atcgcgcagc gtccaacctg caaaacggta atcggtggca aaaccgagga
    30001 acaattgcgg ttcgtagttg gtttcacgaa acggtgaaga ctcttcgcta ttggacagtt
    30061 gccaccagga tttttgcgta taagacgcac ccaacaccga gttcgggcct aaaatcccac
    30121 gccacagcgg aaatgccagg ctcaactgaa actttacttc atccttacgc gcattttccg
    30181 cccagtcgta actggcaatc gcttctttgt tcagatcgct ggtttgggtg tagatgaggt
    30241 agttggtgtc ataaggatag agcgtgaacg gattgtcatg ctcctgcagc atattggcga
    30301 taatactgcc acgcaccgct ggcgcgtcat gcacctcttt caccgttgcc tcttgtgcat
    30361 atactgccat aggcaacata aacaccggca acaaccagcc ctgcagagtc cgcatcggtc
    30421 gtgttctccg taacgaatta ttgataaggt gaatgttttt ctgcgggcta ttttacatac
    30481 ttaactccat tgccgttagt tatcccttcc taaagtagaa accaacaaca aagaagcata
    30541 aaatcaacat ttcattaacc aatgaagtgt aaggatgata tgtctgccgt actgaccgct
    30601 gaacaagccc tgaaattagt gggtgagatg tttgtttatc acatgccatt taaccgcgca
    30661 ttggggatgg aactggagcg ttacgaaaaa gagttcgcac agctggcctt taaaaatcag
    30721 ccaatgatgg tgggcaactg ggcgcaaagc attttgcacg gcggggtcat tgcgtcggcg
    30781 ctggatgtcg ccgccggtct ggtgtgcgtg ggaagtacct taacccgcca cgaaaccatc
    30841 agtgaagatg aactacgcca gcggctatcg cggatgggga ccattgatct tcgcgttgat
    30901 tatctgcgcc caggcagggg cgagcgtttt actgctacca gtagcctgtt gcgtgcaggc
    30961 aataaagtcg ccgtcgcccg cgttgaatta cacaatgaag aacagcttta tattgccagt
    31021 gccaccgcca cctatatggt aggttgaaga cataaaagca ggtaaactgc tgttaccaat
    31081 taaaaatgag tattcccgat ggatgcaaaa caaacgcggc agggcgtatt actcgctctt
    31141 gccgcttatt ttatttgggg tatagcccca gcgtacttca agttgattta ctacgtgccc
    31201 gccgatgaaa tcctgacgca tcgcgtgatc tggtcgtttt tctttatggt ggtgctgatg
    31261 agcatttgcc gccagtggtc ctatttaaaa acgctgattc agacgccaca gaaaattttt
    31321 atgctggcag tctctgccgt actgattggt ggcaactggc tactgtttat ctgggcggtg
    31381 aacaatcacc atatgctgga agcgagcctt ggttacttta ttaacccgct ggtgaacatt
    31441 gtgctgggga tgattttcct cggcgagcga ttccgccgga tgcaatggct ggcggtgatt
    31501 ctggcgatat gtggcgtgtt agtccagctg tggacttttg gttcgctacc tattatcgcg
    31561 ctggggctgg catttagttt tgccttctac ggcctggtgc gcaagaagat tgcggtagaa
    31621 gcacaaaccg gcatgttaat tgaaaccatg tggctgctgc ccgtggcggc gatttacctg
    31681 tttgctattg ccgacagctc aaccagccat atggggcaaa acccgatgtc gctgaattta
    31741 ctgctgatcg ccgccggtat tgtcactacc gtaccgctgt tgtgttttac tgccgctgcc
    31801 acgcgcctgc gtctctcaac gttaggcttt ttccagtaca ttggcccgac gctgatgttc
    31861 ctgctggctg tgacgtttta tggtgaaaaa ccgggtgccg ataagatggt gactttcgcc
    31921 tttatttggg tggcgctggc aatttttgtg atggatgcga tttatacgca gcgtagaacg
    31981 tccaaataaa aaataaatat attctgaaaa aatatgccga ctcttgtatg agagtgggca
    32041 tattgccatg ctgacaggag actcacacaa ggatgtaaaa tttatgttac gtatgtttat
    32101 cccaacatct aacggtaaga tttccagacg ccgatatata ttttcattta ttttaataaa
    32161 tttcattttt tcctttctta ttatcttttt taacgatggg gaagcaggtt ttcttgttat
    32221 agtctctacc atcgtactcc attatctcgt catcaacatg aattgccagc gactccgtga
    32281 tagtggtttc atctatataa aaacttatgt ttttggtaca ttagcagtat atatcatctc
    32341 tatcatcaca atgatagccg aagattttgc ctgttcaggc aatggttcga tgattttttt
    32401 gatttgttat ttttccactt tcagtatgct aatgcttgca cctactgatt catcaaaaca
    32461 atagttatgg agtatttatg agtaaggaat atatgaacga tggttcatta agtgaaaaat
    32521 ggaaataccg atttaatttt tatgatcaac atggctttcc tggattctgg ggagcaacac
    32581 cagaatataa agcagcattt aaagcattaa aagtaagaca acgattaact attcaaatga
    32641 atttcattgc tttcttctgt tcctggatct atttatttgt tctgggctta tggaaaaaag
    32701 caattatagt tctattactt ggcattctat ctctatttgt tggtgcatta attggcgtta
    32761 acattctggg aattgccgta gcagcctatg ttgctgttaa cacgaacaaa tggttttatg
    32821 agaaagaggt aaaagggtta aatacctgga gcctttgaat aacaggtggc ttagccaggc
    32881 taaaccaccg ctctcgtttt ttacaaccag ttcttccgct taaagtacag atacggtgcc
    32941 aggcccgcga ggatcataaa gataatcgcg cctgggtagc cgaaactcca cttcagttct
    33001 ggcataaact caaagttcat cccgtagctg gaagcaacca gcgttggcgg caggaacacg
    33061 acggatacca ccgagaaaat tttgatgatg cggttctgct cgatgttgat aaaacccatt
    33121 gccgcctgca tcaggaagtt caccttctgg aacagggatt cgttatgcgg cagcagggat
    33181 tcgatatctc gcaggatttc acgcgcctgc tccagttgac cacccggtaa acgcgcctta
    33241 cgcaccagga agttgagcgc acgctgggta tccatcagac acaggcgcac tttccagccg
    33301 atatcttcca gttccgccag agtggagagc gcctcgtcgt actcatcgcc ctgatgccct
    33361 tccataatca cccggctcaa ctgctccagg tcgctataga tattttcaat ttcatctgcc
    33421 aactgttcga ttttggtttc gaacagatcc agcagcagct cgtaggcgtt accgtccacc
    33481 attgactggc tacgggcgcg catacgatac agacggaatg caggcagttc acgctcacgc
    33541 agagtaaaca gacgaccatc acggatggta aatgccacag tggagttacc ggcgtgatct
    33601 tccgcatctt caaagaagaa gaaggagtga atatgcaggc cgtcatcgtc ttcaaagaaa
    33661 cgtgccgatg cttcgatgtc ttccagttcc gggcgggttg ccaggctctg gccaagttca
    33721 gattgtacgc gcagtcgctc gtcgtcgtcc ggttcgacaa gatcaatcca tactgcattt
    33781 acaaggggtt gtgactcttc gacttccagc cgggtcagtc ggttattttc cagttgaaat
    33841 gcgctcagca tgaccgggac tcccaatgct taaaatatcg gacagttcgg tgggcacaca
    33901 gaaacaaatt gggtttcaga ccattaaaca gcctgactca gcgcgacggg aaaaattgag
    33961 gtcgctgaca accgctaagg ctatcagcaa aaagggatag ccttaggagt tgatcctgga
    34021 tgacaggata gtgagccagt atctactggg tgtgtccaag gcgaatgtcc tcttagagtg
    34081 atcgtgcgcg catgttacgc cagcaaaatt ttgccgtcaa cacgcaacag aacaccacag
    34141 aacaatagtt gaacttcgcg ctttttggta ctcgtcaagg tcgctaacag gataaaaaaa
    34201 tcccacatcc aggacctata atggcgcatc ggctactcat tggtaaagga atgatcacct
    34261 tgaatctcaa acggattttt ctcgccctca ccttacttcc cttgtttgct gttgccgctg
    34321 atgattgcgc actctcagat ccgacgctga ccgtacaggc gtataccgtt aatcctcaaa
    34381 cagagcgggt gaaaatgtac tggcaaaaag ccaatggcga agcgtgggga actttacatg
    34441 ctctgctggc ggatatgaat agtcagggtc aggtgcagat ggcgatgaac ggcggcatct
    34501 atgatgaaag ctatgcgccg ctcggtttgt acatcgaaaa cggtcagcag aaggtggcgt
    34561 taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg ttttatgtcg
    34621 cgggagataa agtcggcatc attcgtctgg atgccttcaa aaccagtaaa gagattcagt
    34681 ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg cgtattcatc
    34741 ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat gggaatgccg
    34801 tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat gccaaagcga
    34861 aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg tatgtgaaag
    34921 gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgcc gagcggaaag
    34981 gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac agctggaaaa
    35041 tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt atttttatta
    35101 acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca aactgatttt
    35161 agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt tgaccataat
    35221 cttaaaatct ttgatttaac acacgaaatt ccaccgtata acatctggga aggcgcttac
    35281 cgcctgtatc agaccgccag ttattggcca caaggttcgg tgtttgttag cgtggtcgat
    35341 ccaggtgtag gtacaggccg taaatcggta gtgctgaaaa ccaaaaacgg tcaatacttc
    35401 gtttcaccgg ataacggtac gctcactttg gtggcggagt cgctgggtat tgagagcgtc
    35461 cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata taccttccat
    35521 ggtcgcgatg tatatgccta cacaggggcg cgtctggctt ccggcgcgat ttcctttgag
    35581 caggtggggc cagagcttcc ggcaaaagtt gtcgaactgc cttatcagaa agcgaaagcg
    35641 acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg taacgtctgg
    35701 agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga tacgttatgt
    35761 gtgaccattt ccgaaggttc cgagaaaaaa tacgaaggga aaatgccgta tgtcgccagc
    35821 ttcggcgatg tgccggaagg acagccgatg gtgtatctga acagcttatt gaatgtttcc
    35881 gtggcgctga atatggataa tttcgcgcag aaacatcagg ttgcgtctgg tgctgactgg
    35941 aatattgatg tgaagaaatg tgataagtaa ggtgctgatg ccggatgcgg tgtggcgcac
    36001 cgcatccggc aacgttatac cgtctccagc cgggcgtaag ccgccaccag ccatttaata
    36061 ccctggccct ggaatgccac ctgcaaacgg ctatgctcgc cgctgccttc catattgacg
    36121 atagtgcctt caccaaactt ggcgtggcgc acgcgctggc caagtttgta gccgctgtcg
    36181 ttctcgacca tcggcgtacc catacgctga tggctgaccg ggcggcttac cgtggcgcgc
    36241 aggcgcacct cttccacaca ctcttccggc aactcaccga taaagcgcga cgggcgatgg
    36301 taaacctctt tgccatacag acggcgagtt tccgcgtagg tcagcgtcag tttctgcatc
    36361 gcacgggtta cgccaacgta ggccagacgg cgttcttctt ccagacgacc gccttcatcc
    36421 agcgacatct ggcttgggaa catgccctct tccataccga cgataaacac ctgcgggaac
    36481 tccaggcctt tcgccgagtg tagcgtcatc aactgcaccg cgtcctgcca ggtatccgcc
    36541 tgcccctcgc ccgcttccag cgccgcatgg gagaggaacg cctgcagcgg cattaaatct
    36601 tcgtcttctt cgttgtagct gaactggcgc gttgccgtca ccagttcctc taagttttcg
    36661 atacgcgtct gacctttttc gcccttctcc tgctcgtaca tggtacgcag gccggagtct
    36721 ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg cagtttcctg cgctaaggcg
    36781 tcgatcagtt ccataaaccg ctgcaaggca ctggctgcac gtccggcgag ggctttttcc
    36841 tgcaacagtt cacgacatgc ctgccagagt gttaactggc gatcgcgcga tgtctgacgt
    36901 accacgtcca gcgtccggtc accaataccc cgcgttggtg tattcactac gcgctcaaag
    36961 gccgcgtcgt cgttgcggtt ggcaatcagg cgcagatacg agagcgcatc tttgatttcc
    37021 tggcgttcga agaagcgcat cccgccgtaa atacggtacg gcatactcgc ctgtaataag
    37081 gcctcttcca gtacacgcga ctgggcgttg ctgcggtaga gaatggcgca ctcggcaagc
    37141 gctccgccgt tgtcctgcca ggttttgatg cggttaacca caaaacgcgc ttcatcgagt
    37201 tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg cgccatcggt ccacagtttt
    37261 ttacccagac gcccgttatt gttttcaatc agggcgttag cggcgctcag aatattgctg
    37321 gtagagcggt agttttgctc cagacgaata gtttcggcac cggggaaatc attaaggaaa
    37381 cgctgaatat tctccacctg cgccccgcgc cagccgtaga ttgactggtc gtcatcaccg
    37441 acgatcatca ctttgccggt gtcgcccgcc agcaggcgga tccacgcgta ctgaatgttg
    37501 ttggtatcct ggaattcgtc caccaggatg ttagtaaaac gttcgcggta gtgttgcagg
    37561 atatgcggct tgttaagcca caactcgtga gcacgcagca gcagctcggc gaagtccacc
    37621 aggcccgcac ggtcgcacgc ttcctgatac gcctgataca ctttctgcca agtctgctcc
    37681 accggattac cgtagctttg aatatgatgc ggacgtaagc cttcatcttt ctggctgttg
    37741 atgtaccaca ttgcctggcg cggcggccac tgcttctcgt cgaggttcat ggctttgatc
    37801 agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga tctggaaatc ctgcggcaga
    37861 ttggcgtcca tatggtgcgc acgcagcagg cggtgcgcca gcccgtggaa ggtgccgacc
    37921 cacatgccgc cctggctggt gcccatcagt tgcccgatac gatgacgcat ctccgccgcc
    37981 gctttgttgg taaacgtcac agccataatc gagtatggcg agcagttttc cacgctcatc
    38041 agccaggcga tacgatgcac cagtacgcgc gtcttaccac tgcccgcgcc cgccagcacc
    38101 agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt tgtcattaag gctgtcgagc
    38161 aggtaagaaa cgtccattgg caccgccgcg taaaaatagg ttgggcggag aagcgcaacg
    38221 ccctccgcca aaaagctggg tatatataca gatttgctga ttatatcagc gaggtcagag
    38281 atgccaaccg ggaaatttcc agatgcggca gtaaacggct atcccaggtt tgcatcagat
    38341 cgccattttc cggtctgatc caacaagcct gcattccgct gcgaattgcc ccacccacgt
    38401 cagtggtgag atcgtcccca acatgtaaga tctcgccgat cggcacgttg agtttttccg
    38461 cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc gtgcgggcca gcgcgcagca
    38521 caaactcaaa ataatccccc aaaccaaaca gttccggctg ggcgttaccg ttggtgatcg
    38581 ccaccagcgg ccattttttc gccagctgtt ttaaggtgtc gtgagtttgc tgcgggacgt
    38641 cgattcggct gcgccatttg gcaaagttaa tcattgctgc gtgtgcgcct gcactggctt
    38701 cttcggcact cagcccggcg tcgagcatcg cttgttcaat cgaacgaaaa cgccaacgcg
    38761 tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc ctggcgcagg cgttgcagat
    38821 cttcattctg gaagctgcgc agcgccggat gataattttg cacaaaggta agcgcctctc
    38881 gctcggtgcg caaaatcacc ggacggttat cgtaaagggt atcatccagg tcaaaggtga
    38941 gcgccgagat gcgccccaaa ggccggtaaa aacgcattat ttcccccgtt tggcgcgtgg
    39001 atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca agatgagtat agatttgcgt
    39061 ggtggagagg ttggcatgac ccagcagctc ctgcacacca cgaagatcgc cgctcgactc
    39121 cagcatatgc gtagcgaacg agtgacgtaa tttatgcggg tgaacgtgat tattcagccc
    39181 ttgttttatg ccccattcgg caaagcgttt ctgcacatta cgcgcggaga tacgcttgcc
    39241 cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac aggtcgcgca aatcaagcca
    39301 gtgctcaatc cacgccacag cgttgcgacc aatcggcagg cggcgctctt tgctgccttt
    39361 ccccattacc cacacttcac cagactccag gtcgaggtgt ttgatatcca gccccaccaa
    39421 ctcggaaaga cgcagaccag cgccgtacat cacttccagc attgcacggt cgcgtacagc
    39481 gaggggatcg ttgatatcaa tatccagcag ccgattcata tcgtcgacgt cgatgttttt
    39541 cggcagatga cgcggcgctt tcggtgccga aacacctttc gccgggttag ctttgagttc
    39601 gttctggctg accagccagt caaaaaagct acgtagcgca gaaagccgta acgcgaggct
    39661 tgctgctccc agccctttac ggcgactgcg tacagcaaaa ttgcgcacca tcgctgcatc
    39721 gcattgttgc cagctttgca ggccgttttc gctggcaaaa ttgatgatcg cctcaagctg
    39781 acgctggtag ttaagtaggg ttatcgggct aagctgacgc tccacgctca gataacgtag
    39841 gtagcgttct acatcggtgt gtaaatcggt catacgcgtt caatccaacg ctccagaagc
    39901 tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc cctgcccttg ttgatagtga
    39961 ctggcatcgc gactggtaaa cagcacgaca cccaaatcag catcgcttcc cagcatcgac
    40021 atcgccaccg atcccaccgc tttcgcttcc ggtagcacca ccagcagctc tggtccgtta
    40081 agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa tacgcagcgg ttcgaaagac
    40141 tgacggctta atgccagatg agtgtggttc gacggcgcac ctaagcgcca gcgatccgga
    40201 aacaggcgca gactcgcacc tgccaggccg agatcgcgcg cccagcggtg aaagcgcatc
    40261 agcatatcgt cgagactgct ggcggcggtg agactgcgct gcaggtagag taagcgataa
    40321 aacaggcctt cgttggcgat agcctgttcc atcaacagcg ccatgttctc ttccagaaca
    40381 tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca acgaaacggt gccgcgtacc
    40441 ggatgcggca cacgtatcgc ttctactgcg cgcgcattgc ggataaaaaa ctcaggattt
    40501 ttaatcagat aatcgacaac cgcccggtca tcaagctccg tgagtgtttc ctgcagttct
    40561 tcccttggtt gcttcataga tgaataaatc cgtcgtagac atgtaccgcc gggccagtca
    40621 tatataacgg gtgacccgga cctttccagg cgatatcaag acgaccgccg gggagttcca
    40681 cgcgtacttc ttcggccagc aaaccttgct gaatccctac cgccaccgcc gcacatgcgc
    40741 cgctgccgca ggcctgggtt tctcctgccc cacgctcata aacgcgtaaa cgaatatgct
    40801 cgcgcttaac cacttgcata aaaccgatat tggctcgctc cggaaaacgc tcgtggcttt
    40861 ccagaacagg accaagcgtt tctaccgccg cggtatcgac atcatcgacc tgaatcacgc
    40921 aatgcggatt tcccatcgac accacgccgc ataagattgt ctgctcggcg gcgcgcataa
    40981 tgtaggtctt ttccgctttg ttagcgcgaa acggcacagc ggaaggttcg aagttgggtt
    41041 cgcccatatt tacgcggacc agatcatcat cggtgacggt cagaaccatc cgcccgttgg
    41101 cggtgctgac gcggatatcg cgcttattgg tcagcccttt caggcgcaca aaacgggcaa
    41161 agcagcgcgc accgttgccg cactgcgcca cttcactgcc atcagcattg aaaatgcgat
    41221 agtgaaaatc cagttcagga tcatacggcg gctcaaccac cagcagctgg tcaaacccta
    41281 cccccaggtg ccgatacgcc aggcgacgaa tcagctccgg tgaaaaaaag acattctgcg
    41341 ttaccgcgtc gacgaccata aaatcgttgc caaggccatg cattttcgag aactgcatca
    41401 tttactccaa tcacgcgggt acagaaactg acttttagta attcacctgg gatggaccat
    41461 cgccagtggc gcgatcgttt ttatccggca ccgtggattg cgtttgcgtc tctaccggtt
    41521 tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata gagcggacct ttcagaccgc
    41581 agcccgtcag gctgaagaga gtaagtaata cagtgagtgc cttaaacacg tttttcatta
    41641 tagattgcct gtaaagttca tcgctttctg ctttctatca tcgcaggaga aggcgtaaaa
    41701 gcaagcgttt agcgactctt tatgaagtct gaaaagtgag gaaatgctgc gagtataacg
    41761 cttactcgtg gggatttatg ttttgctggt agtttcgtga tcggcacctc gcgccaggat
    41821 ggctttatgc actatcgcaa tgtggtttgt ttgttggctt ttttagttcc gtcagcgtga
    41881 ggatgctcgc tcggtaaacc tgatcataat gtttaccaga aacagggaaa ggcgtggtgt
    41941 aactgcgccc taatgaagag acaattcttt cgctacacag agacatcatt attccggtga
    42001 cggatgaatc atgccgtaac gcagccgcag aggtgcaaac tttgaagccc tgaccccatg
    42061 ccgctatact gccgccatca cttaaaaaca ggatacaaca atgaacgaca gtgaatttca
    42121 tcgcctggct gatcaactgt ggctgaccat tgaagagcgt ctggacgact gggatggcga
    42181 cagcgatatc gactgcgaaa tcaacggcgg tgtactgacc attacctttg aaaacggcag
    42241 caaaatcatt atcaaccgcc aggaaccgct gcaccaggta tggctggcaa ccaaacaagg
    42301 cggctaccat tttgatctga aaggcgatga gtggatttgc gatcgcagcg gcgaaacctt
    42361 ctgggatttg ctggaacagg cggcgacgca gcaggcgggt gaaacagtca gattccgcta
    42421 agattgcatg ccggataagc ctcgctttcc ggcacgttca tcacgaaaaa tactgctgta
    42481 atagcggcgt atcgtgatcc tgattggcag gcggcatgtt accgatagat tttgtgcgga
    42541 acggaatcac ctgttcacga ccatcaacct tcacaatctg atagaactgc ggcaggttga
    42601 agttgatgaa gcttgagccg taggtaaaac ggtcatgcga tgacgagtag aagcgactga
    42661 cgtcacgtac cagctcctct ttgctgcctt cgcagtggtg atacacctca acccggttgc
    42721 tttcgtcgag aatgtagata ttaaagccat tctcatcttg cgtttcttcg aaaaagaact
    42781 ggatgatccc ttcgctggca aagccgtcca ccaccgccgg taatttgacg tgattggttt
    42841 caacctgcac tgacaggccg tgcagtttgt tatgcgaaat agcgccataa aactcgatgg
    42901 cgttctccag tttctgtacc gatacattca ggcgttcgaa gaacaagccc caggtttgac
    42961 cagaaacgcg cagcgcctta aaacgcccgg tttcctggcg ggtgctggaa agacgcaatt
    43021 caatacactc agaaaccagt tgctgcacgc gagtacgaat taagccgcgc agatgctggc
    43081 tataacagaa gacttccacg ctatctggcg gtgcggcgtc ctgatgcatt ttgccgagaa
    43141 tagttttcag ggcttcgatc atcgattgct cgccgttgaa gtgcagcgta cgcacttcgt
    43201 tccacgagtt gcggtacagc aggtcaacgc tacctaccag gcaattttga ttctcgccaa
    43261 agctgaagac atccagctta cggaaatcga aatgcaccac ctgattgcgg aacgccgctg
    43321 tcgggtcata ttccaggtta acgataatcg ccagatggcg gatctcacac gggctataga
    43381 gcgccttcgg tgtcggtgca ggtaagcgca gcgggaaatg gtgcgacacg tcggcgacca
    43441 tctcctgcaa cttaggcaaa tcgacaatgc cgttaccttt aatatacaaa cgggtgcgcg
    43501 aggtcagcag gccattaaac catgcccacg ccaccagctt attcaggtaa cggttatatt
    43561 ccagcggctg atggctgatg atcgactcaa tatttggcgc gcggttatac agataccaac
    43621 ctgaacggtt agcccggtcc ggcggcacat aaataaaggt cagattcggt tccgagagat
    43681 cgggtgaaat ctgcgggttt accagcgtca ctttacctgg taatgcttca aacgcggcat
    43741 acagcttacg cgtcagcacg ccgatatcct gcggactggc ggagacgcta aggttattgc
    43801 gacgcgcaaa gcggatcaga ttacggtagc tctgcatcat cgcgtcgagc aactcgttgt
    43861 gcgcctcacg cacctgatca atcttccagt tagcgcggtt atcgagcatt gccagacgag
    43921 cttcgtccca accccactcg ctcactaact ggctcaacac tgcgcgacgc cagcctacgc
    43981 aggcgcgttc acggctgagc ttttcgcaca cttttaaata gaagcagcga cgtactaaat
    44041 ccagacgggt aaaatcttca atcgccgtca ggtattcagt aacacgctcc agcatcatgc
    44101 agtatggatc gagaccaaac gatacaatct cgccgtcgtg caaacgctgt ttgatatctt
    44161 tcgccagcag acgtgggttc gggtattccc aggaataggc ttccagcagc agtgttttca
    44221 gtaccgcttt gtatggggaa tcgatactct tgtagagctg ccaaaggctg gcaccaaagt
    44281 actcttcagc agaaagcgag cttaagccac cgagatccag ccattcattt ggcgtcagca
    44341 cgccttgtgc gtagagcgtc attacgtagt catcgtaatg ctcttcttcg tcgcacggca
    44401 ccatattcca cagaatacgc ttaccggcga gacgcacggc ggtacgataa aattcatcaa
    44461 gcagcagtat atgctgggtg gagccacaat cttcgccccc cagactgcca ctctcgttgt
    44521 ggcgaaaacg gttttcatca atcaggaaga agctaacttc cacacccagc gaggcggccc
    44581 agctttccag caggctgcat ttacgttgta gcaactggcg ctcttcgcta tcgagccagg
    44641 attgatgaca gacccagata tccaggtcag aggaacaact ttgccctacg gacgaggtgc
    44701 tgcccatggt gtatacacca gtaattggaa gctcaccttt cggcgggtcc tgtactgaca
    44761 ttccacgata cagttcaagc tcgttcaggt agtggcgttg agtttcatca ggcgtgtaaa
    44821 ggcaaatgcc tttgggaacg ttaccatcaa ggtaacccgg cattagcgga tggtgatagt
    44881 gcaacaatgt cggcagtaga ctgtagacct gttggaatgc aggccccata gcagcaagcg
    44941 cgcgatccac acgcaattga tttatggcat ccagtctctg tttcagagtc tcaatataga
    45001 ggtacaagac gtatcgcctg atttgctacc cgtcatgact gtgattccgc caacatcaac
    45061 ggtaacacgc ggcattcggg atatttcgta tgtcaaaggt aaccgttacc acttttcgcg
    45121 cctggttttt ttagtttcac gacgaaaaaa tggtctaaaa cgtgatcaat ttaacacctt
    45181 gctgattgac cgtaaagaaa gatgcgctac atacaagtgt agcaccgttt attctctgta
    45241 aattccttat tacgacggcg tgaaacgcct gtcaggatcc actgccagac ctcattttac
    45301 ggtttgcgca ggcgtctacg tttcaccaca acactgacat cactctggca aggatgttag
    45361 gatggaccac ggatgataat gacggtaaca agcatgttag acaatgtttt aagaattgcc
    45421 acacgccaaa gcccacttgc actctggcag gcacactatg tcaaagacaa gttgatggcg
    45481 agccatccgg gcctggtcgt tgaactggta ccgatggtga cgcgcggcga tgtgattctt
    45541 gatacgccgc tggcgaaagt cggcggaaaa ggcttatttg taaaagagct ggaagtcgcg
    45601 ctcctcgaaa atcgcgccga tatcgccgta cattcaatga aagatgtgcc ggttgaattc
    45661 ccgcaaggtc tggggctggt cactatttgc gagcgtgaag atcctcgcga tgcctttgtg
    45721 tccaataact atgacagtct ggatgcgtta ccggcaggca gtatcgtcgg gacgtccagt
    45781 ttacgtcgcc agtgccaact ggctgaacgc cgcccggatc tgattatccg ctccctgcgc
    45841 ggcaacgtcg gcactcgcct gagcaaactg gataacggcg aatacgatgc catcattctt
    45901 gccgtagccg gactaaaacg tttaggtctg gagtcccgca ttcgcgccgc gttgccaccc
    45961 gagatttctc ttccggcggt aggacaaggt gcagtcggta ttgaatgccg ccttgatgat
    46021 acacgcactc gcgagctgct tgccgcgctg aatcaccacg aaactgcact gcgcgttacc
    46081 gcagaacgcg ccatgaatac ccgtctcgaa ggcggatgtc aggtgccaat tggtagctac
    46141 gccgagctta ttgatggcga aatctggctg cgtgcgctgg tcggcgcgcc ggacggttcg
    46201 cagattattc gcggtgaacg ccgcggtgcg ccgcaaaatg ccgaacaaat ggggatttcg
    46261 ctggcagaag agctactgaa taacggcgcg cgcgagatcc tcgctgaagt ctataacgga
    46321 gacgccccgg catgagtatc cttgtcaccc gcccgtctcc cgctggagaa gagttagtga
    46381 gccgtctgcg cacactgggg caggtggcct ggcattttcc gctgattgag ttttctccgg
    46441 gtcgacaatt accgcaactt gctgatcaac tggcagcgct gggggagagc gatctgttgt
    46501 ttgccctctc gcaacacgcg gttgcttttg cccaatcaca gctgcatcag caagatcgta
    46561 aatggccccg actacctact tatttcgcca ttggacgcac caccgcactg gcgctacata
    46621 ccgtaagcgg acagaagatt ctctacccac aggatcggga aatcagcgaa gtcttgctac
    46681 aattacctga attacaaaat attgcgggca aacgtgcgct gatattacgt ggcaatggcg
    46741 gtcgtgagct aattggggat accctgacgg cgcgcggtgc tgaggtcact ttttgtgaat
    46801 gttatcaacg atgcgcaatc cattacgatg gtgcagaaga agcgatgcgc tggcaatccc
    46861 gcgaggtgac gacggtcgtt gttaccagcg gtgaaatgtt gcagcaactc tggtcgctga
    46921 tcccacaatg gtatcgtgag cactggttac tacactgtcg actattggtc gtcagtgagc
    46981 gtttggcgaa actcgcccgg gaactgggct ggcaagacat taaggtcgcc gataacgctg
    47041 acaacgatgc gcttttacgg gcattacaat aactctcata acaggaagcc ataatgacgg
    47101 aacaagaaaa aacctccgcc gtggttgaag agaccaggga ggccgtggac accacgtcac
    47161 aacctgtcgc aacagaaaaa aagagtaaga acaataccgc attgattctc agtgcggtgg
    47221 ctatcgctat tgctctggcg gcaggcgtcg gtttgtatgg ctggggtaaa caacaggccg
    47281 tcaatcagac cgccaccagc gatgccctgg ctaaccaact gacggcattg caaaaagccc
    47341 aggagagcca aaaagccgag ctggaaggca ttattaagca acaagctgca caacttaagc
    47401 aggcgaatcg tcagcaagaa acgctggcaa aacagttgga tgaagtccaa caaaaggtcg
    47461 ccaccatttc cggcagcgat gctaaaacct ggctgctggc tcaggccgat tttctggtga
    47521 aactcgccgg acggaagctg tggagcgatc aggacgtcac gaccgctgca gcgttgctga
    47581 aaagtgcaga cgccagcctg gcggatatga atgacccgag tctgattacc gttcgtcggg
    47641 caattaccga tgatatcgcc agcctttctg cagtatcgca ggtggattat gacggcatca
    47701 tccttaagct taatcagctt tcaaatcagg tagataacct gcgtctggcc gataatgaca
    47761 gcgatggttc gccgatggat tccgacggtg aagagctttc cagttccatc agcgaatggc
    47821 gtatcaatct gcaaaaaagc tggcagaact ttatgaacaa cttcattacg attcgccgtc
    47881 gtgatgacac cgccgtaccg ctgttagcgc caaatcagga tatctatctg cgcgaaaata
    47941 ttcgctctcg cctgctggtc gcagcacaag ctgtaccgcg tcaccagcaa gagacttatc
    48001 gccaggcgct ggagaacgtc tccacctggg tacgtgctta ctacgatact gatgatgcca
    48061 ccaccaaagc gttcctcgac gaggtggacc agttaagcca gcaaaatatc tcgatggatc
    48121 ttccggaaac cctgcaaagc caggcgatgc tggaaaaact gatgcagact cgcgtgcgta
    48181 acctgctggc acaaccggca gcggggacaa cggaagctaa acctgcacct gcaccgcaag
    48241 ctgcaccgca agctgcaccg caagctgata ctccggcagc cgcgccgcaa ggagaataac
    48301 catgctaaaa gtgttattgc tctttgtgtt gctgattgcg gggatcgtgg ttggcccgat
    48361 gattgccggt catcagggtt atgtgctgat ccagaccgac aactacaata tcgaaaccag
    48421 cgtcacgggc ctggcgatca tattgattct ggcgatggta gtgctgtttg ccattgagtg
    48481 gctactgcgg cggatcttcc gcactggcgc gcacacccgt gggtggtttg tcgggcgtaa
    48541 gcgtcgccgt gcacgtaagc agaccgaaca ggcgctgctg aaactggcgg aaggcgatta
    48601 tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac gctgaacaac cggtggtgaa
    48661 ctatctactg gctgccgaag ccgcgcaaca acgtggtgat gaagcgcgcg ccaaccaaca
    48721 tctggaacgc gcagcggagc tggccggcaa tgacaccatt ccggtagaaa tcacccgtgt
    48781 acgtctgcaa ctggcccgta atgaaaacca tgctgcacgc cacggcgtgg ataagctgct
    48841 ggaagttacg ccacgccatc cggaagtatt acgtctggcg gaacaggcgt atatccgcac
    48901 aggtgcatgg agttcgctgc tggatattat cccatcaatg gcgaaagccc atgttggtga
    48961 tgaagaacat cgtgcaatgc tggaacaaca ggcatggatt ggcctgatgg atcaggcgcg
    49021 tgccgataac ggtagcgaag gtttgcgtaa ctggtggaaa aaccaaagcc ggaaaacgcg
    49081 tcatcaggta gcgttgcagg tggcaatggc ggaacatctt attgaatgtg acgatcatga
    49141 tactgcccag caaattatca tcgatggcct gaaacgccag tacgacgatc gcctactgct
    49201 gccgattcct cgactgaaaa caaacaatcc ggaacagctg gaaaaagtgc tgcgccagca
    49261 aatcaaaaac gtcggcgatc gcccgctgtt gtggagcaca ctgggccagt cactgatgaa
    49321 gcacggagaa tggcaggaag catcgctcgc cttccgcgca gcgctgaaac aacgtccgga
    49381 cgcctacgat tacgcatggc ttgccgacgc gctggacaga ctgcacaagc cggaagaagc
    49441 tgcagcgatg cgtcgcgacg gtttgatgtt aacgttgcag aacaacccac cacagtagtt
    49501 ccttctcacc cggaggcaag cacctccggg gccttcctga cacataaaaa aacgcctgct
    49561 cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa caagtgggtg cttcactcaa
    49621 cgttgtgtcc atggtgtctg atgaggcata agcgacatct gtcagtggac gataagcacc
    49681 gtaaacggct ctgcgtcatt ccggagttta tgaggcacta aggcgaacat aagagatgga
    49741 atgagcatct actcgtttat tatgccacag agaatcggga aataacatcc cttaacactt
    49801 gttatgagat aattctgtaa tcctcttggc ttcctgagta ataacttcct gagtgaatat
    49861 ttaccctgag cttgattcta cacatactca ttatgaatga taaaattcat tcaattaata
    49921 acacatatat taattgccgt taaaactaaa aacagcatca ataatcaacg cgatataata
    49981 aacctgcctt acatatcaac tgcgccagag gtaggattga aaacgctctc ctgattttcc
    50041 aattcatttt ctggataaat aaataattta tttttgtcac tattatttat gtaatcatcc
    50101 tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt gatgacatga
    50161 aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cgtcttgttg ttgctgtcgc
    50221 cgccgctctt cctctcatgg ccagcgcagc agataccccg tcaactgcca ccgcacgcaa
    50281 aggctttgcc ggatacgatc acccaaacca gtatctggtt aaaccggcga ccactattgc
    50341 cgacaacatg atgccagtaa tgcagcatcc ggcgcaggat aaagaaaccc agcagaagct
    50401 ggcagaactt gagaaaaaaa ccggtaagaa accgaatgtg gttgttttct tgctggacga
    50461 tgtgggctgg atggacgtcg gttttaacgg tggcggcgtg gcggtgggta accctacacc
    50521 agatatcgac gccgttgcca gccaggggct gattttaact tcggcgtatt ctcaaccaag
    50581 ctcttcccca acccgcgcca ccattctcac cggacaatac tccatccacc acggcattct
    50641 gatgccgcca atgtacgggc aaccgggcgg gctgcaaggg ttaaccacgc tgccacagtt
    50701 gctgcacgat cagggctacg tcactcaggc catcgggaaa tggcatatgg gggaaaacaa
    50761 agagtcgcag ccgcagaacg ttggctttga tgatttccgt ggctttaact cggtgtctga
    50821 tatgtacacc gaatggcgcg acgttcacgt caatccggaa gtggccctga gtccggaccg
    50881 ttctgaatac atcaagcaat taccgttcag caaagatgac gttcatgcgg tgcgcggcgg
    50941 cgaacaacag gccattgccg atattacgcc gaaatatatg gaagatctgg atcaacgctg
    51001 gatggactat ggcgttaagt tcctcgacaa gatggcgaag agtgataagc ctttcttcct
    51061 ctactacggc actcgtggct gccacttcga taactaccca aatgcgaaat atgcaggtag
    51121 ctctccggca cgcacctcgt atggcgactg catggtggaa atgaacgatg tgttcgctaa
    51181 tctgtataaa gcactggaga aaaacggtca gcttgataac acgctgattg tctttacctc
    51241 cgataacgga ccggaagccg aagtaccgcc gcacggacgc acaccgttcc gtggtgcgaa
    51301 aggatctacc tgggaaggcg gcgttcgcgt accgactttc gtgtactgga aaggcatgat
    51361 ccaaccgcgt aaatctgacg gtattgtcga tctggcagat ctcttcccta ccgcgctgga
    51421 tctggcaggg catcctggcg cgaaagtggc gaatttagtg ccgaaaacca cctttatcga
    51481 tggtgtggac cagacatctt tcttcctggg aacaaatggt cagtctaacc gtaaggccga
    51541 gcactacttc ctcaacggta aactcgctgc tgtgcgtatg gatgaattca agtatcacgt
    51601 cctgattcag caaccttacg cttataccca gagcggatat cagggtggat tcaccggcac
    51661 agtaatgcaa acggcgggat cgtcggtgtt taacctgtac accgatccgc aggaaagcga
    51721 ctccattggc gtgcgccata ttccgatggg tgtaccgcta cagaccgaaa tgcacgcgta
    51781 tatggagatc ctgaaaaagt atccaccacg cgcgcagatt aaatctgatt aagccggcgc
    51841 tatcgctgag atctgccttt gccggatgcg atgctgacgc atcttatcca gcctacagaa
    51901 cgctgcaatt tattgaattt gcacgatcat gtaggccgga taaggcgttt acgccgcatc
    51961 cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt tacttactca ccaccagcaa
    52021 atgcgcatgc ataatgtcgc tggccggacg accgtgcgcc agcaaatcag ccatagcttt
    52081 aagatatggc ggtagatggc ggaaataacg ctgatacccg gcacacaaat aattcagccc
    52141 cggtttgccg ctggcatcga gcatgaagcg gtgtttcgga cagcctcccc aacatgcttt
    52201 taacacgtta caactgcgac actgcgccgg taactgctta aatttatctt caccaaaagc
    52261 ctgctgctgc ggagaatcta ccatttctgc aattgtttgc tggtgcatat tccccagtcg
    52321 atattgcgga taaacatagt gatcgcaggc gtaaacgtcg ccgttgtgct caacaatcac
    52381 cgagcgccca caggttggct gatgatggca aaccgcaccc ggcgcaccga caaaattggc
    52441 aaacgcccat tcgatattca tcacaaaaat cttgccgaca tcacgtttga tccagtggtc
    52501 gaaaatcgcc accagaaatt caccaaattc atcggggcgc accgaccatt ccgttagctc
    52561 accctgaata tcaccaggcg catgcaattt cagtccttcg cgggcagccg tttcatcagc
    52621 caagcgctcg accaccggaa taaactggat gaattccact cccgcatcgc acaaaaaatc
    52681 atatacctgc aacggttgct gcgcgctggt gcgattaacg cagaccagca cgttatagtc
    52741 gacatgatgt ttttgcagca gcgtcagggc acgcatcacc agcttatgcg ttggtctgcc
    52801 acctttggtc acgcgatatt gattgtggat ctcagccgga ccatccagcg ataacccaac
    52861 aagaaaatga ttttctgcca gaaatgcaca ccattcgtca tcgagcagca cgccgttagt
    52921 ctggaagctg ttacttatct tcctgccagc accatatttc gcctgtagcg ctacggcacg
    52981 gcggtaaaac tccagcccga gtagcgttgg ttcgccgccc tgccaggtaa aagccacttc
    53041 gttttgcggt tcgctggcag cgatatagta acggacatac gcttccagcg tgtcatcgtc
    53101 catatgcgtg actggttttt cgcggtaaag ggattgtttt tcgagataaa aacagtagtc
    53161 acagttcaga ttacaatcgg aaccgctcgg tttcgccatc acatgaaaag cacgcgttgg
    53221 aacctgttgc agcatggtcg ctccttgcct gaagtcatcc ttcaatacgt taagggcgaa
    53281 atcataccgt ggggcaatga gttattacgg gcgtttattg cgaaggaagg gtgatagaat
    53341 gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt
    53401 cgaacctccg acccactggt cccaaaccag ttgcgctacc aagctgcgct actcgccgaa
    53461 atactgcttt ttgaattttt agttcaattc tttaaagtcg tggtgcgagg ggggggactt
    53521 gaacccccac gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca
    53581 ccttcgcatt gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa
    53641 ctggaatcac aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta
    53701 cgcggtatta ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt
    53761 cgaacctgag acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt
    53821 agcgccgttg cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa
    53881 ataaattgcg cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg
    53941 ctttcccgtg gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga
    54001 tgatgccaac aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc
    54061 aaatcaggaa tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat
    54121 ggctggcaat cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa
    54181 accatggcac catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg
    54241 gattgggaat gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca
    54301 cacccgcaac gggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt
    54361 ttttcgccag tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga
    54421 gcgcagccgt cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg
    54481 caaaagtcag tacgaacggg ctgccgttgc tgcctatttc attccacggg aagatggtga
    54541 cgataacgaa aatcgcgcct acgtagaaaa tcaggatccg ccacagcacc ttgcctacgg
    54601 cactgcgcag cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca
    54661 cgccctggta ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg
    54721 caaagaaacc gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa
    54781 agccaaagaa aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga
    54841 tcatcgcgaa ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg
    54901 ccaccagcgc cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact
    54961 ggacataaac gccaatggcg gtgatttcag agatccccac cgccatccac ataaaccagt
    55021 aagaccaggc ggtgagatag ccaaagaacg ggctcatata acgatgcgca taaacggcga
    55081 acgaaccggt aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga
    55141 cgaacagccc ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac
    55201 tggcggcccc cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat
    55261 gtcgagcttc cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg
    55321 tgtttgcatg ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg
    55381 ttttcgtaat tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat
    55441 aaaaaagcag acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag
    55501 aggttgccgg tgtaatgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg
    55561 ctcggctgcg aaagcaggcg gtagagccac tccagcccca gcgtttgcca gattttcggt
    55621 gcgcgtttca cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca
    55681 tctggatgca ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca
    55741 acggtgacga tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg
    55801 ggtttaaaat aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta
    55861 gcttcagttt gcgccagcac ttcaggttta ccgcccacaa gaaatactgg cgtcccttct
    55921 ttgcctgcgc gcgccatcag ctcttcccaa agatcggcac cggcaacgcg ggaaacctgc
    55981 gcctgcgggt actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat
    56041 tcggcagcgt taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg
    56101 gcattaatgg caaccaacgt tccctgctta agctgcccgt cagcaaacag ataatcgagg
    56161 gcgtgctgca tatcacgcca accgatcaac tgtaagccac gcagcgtata ggtcggtgcc
    56221 gtggtgttgt tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt
    56281 tatgaatgag tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg
    56341 cgccgaagac cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgcg
    56401 ccagcacgat catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat
    56461 agcgattagt ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc
    56521 caaccacaat cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta
    56581 taagcgtagg cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt
    56641 tggctgagtt cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat
    56701 agaaatcgcg gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca
    56761 gcaacgccag attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga
    56821 acgcttcatc gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccaa
    56881 caatccccag cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc
    56941 caataaacag gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca
    57001 tataagtcag caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt
    57061 cctggcgcag aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta
    57121 acgccacgcc ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca
    57181 aaaagccgtt gtgcatgaag aagatgccga cgcttaccag cgcgatgcct atcaggatca
    57241 cccacgtaag attggtttcc acgcggttca tggtaaacag cggacggcgc ggtacatcag
    57301 caacgcgttt gcgtaggcgg gttttgtagg tgacgtagta aaccgcgtag aagcagcccg
    57361 cagaaagcaa cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata
    57421 ccagcacgct ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga
    57481 agacattgaa gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca
    57541 gcgtgcagag cagccagaca acaaacaggc cactgaattg cagcagactc atgcgacctc
    57601 cccggcggca atcgccaacg cccgctgcca gccttgcaga tagttcgggc taaagaaggc
    57661 aatggtgttt ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt
    57721 gagatcgtcg gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg
    57781 attttcccgg ttaagcacac aaggaatgcc cgcctgaatc agtaagcaca gcgtaccaat
    57841 gccctgctgg cgggcaaaaa taaagtaacc gagatcgcac tgacgaagta gcgccagata
    57901 ggcgtcaaat tccagttttt cgctcagaac ttgcagattt tcttcgctga ataactccag
    57961 cccagcctga cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac
    58021 caccactttt accgtatcgc caaattgctg atgaacggcg cgcaaggcag caatatgctc
    58081 attgctgcgg tcgccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc
    58141 gttcgccatc gtattgagag aagagtccat ccgcgtcggg aagtacagca gttcgccccg
    58201 cacctttgga tgcgttttgg caaaaaagct caaatcaccg cgggtggcaa atacacagcc
    58261 gactcgcttt tgcgccaggc gacgtagcgg gtaaaaaagc ttatatctca agccactgga
    58321 aagctcatac agatctgccc cccagatatg ccagaaaaac tggcgggact taatgccacc
    58381 actcagcaaa gccagccaca gtttgggatt gaactgaccg tggaagaaaa aacgctgctg
    58441 acggttagct tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa
    58501 ttgcacagaa agcgccggac agttatcact taagccgtcg tccttgccaa caaccataaa
    58561 ctcgcgcgcg tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg
    58621 gttatggtga gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc
    58681 cagagtaaaa acacgccaca acaaagagaa aaatagacga tataagttgc catataggcc
    58741 tgcgccgcgc ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa
    58801 gtgaactggc tgacttccgc cagaatataa aaccgcagtg aagctttggc gatcaccaga
    58861 taaccaaaga cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga
    58921 tcgcgcatag cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcaac
    58981 cagacggtaa aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact
    59041 tcccgggtga tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg
    59101 aacgatgccg taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc
    59161 cccacttcat cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca
    59221 ggcaaggtca ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga
    59281 ccgttatccc agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg
    59341 atggcggcag gaatcaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca
    59401 taaccgccca aacggtaact gacgtagtac gcgagaacgc caatcaagct gccaacaatc
    59461 agcgataacg cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg
    59521 ccccaggcga tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta
    59581 ttaccaaaca atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc
    59641 gtagagaagc caattaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgggga
    59701 ttatcatggt actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc
    59761 acgccgagca cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca
    59821 aatgacaccg ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg
    59881 gccgccgtcc acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt
    59941 cgcaattacc gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag
    60001 gcgctcgctc tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc
    60061 cgcagggcaa ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag
    60121 aaagttaatc aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc
    60181 gttctgcacg cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg
    60241 tgctaacgca tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc
    60301 tgcttccagt tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc
    60361 aatatcgcgc caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc
    60421 cttttcacgg atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc
    60481 gccttcacca cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc
    60541 aatggttcca agcgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttctac
    60601 cacaaacaga ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc
    60661 cgcgtaatgg accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt
    60721 ttcgtcgatg ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag
    60781 cacaaaggca ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg
    60841 ctggatatcg agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa
    60901 cactttggcg ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc
    60961 atcgccacac agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac
    61021 caccggcggt gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt
    61081 ctacattcgc accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg
    61141 ccacccgcaa agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct
    61201 cagcacctgc accgcgtcca gccagcaggc ctattcgcgc atctgtcgca ttgagttccc
    61261 gtaaagagac atagccgcga atatcgccgg acgccgcacg taaaatcaga cattgatgat
    61321 caaaggtgcc gcgcacggca ttttcaatcc actgtgcata aaagcgactg ctggcgtcag
    61381 gcgcatacca cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta
    61441 atgcgggaat atcggtctct tgtgccacta cagcaccgct atcactggca ttgttcacgg
    61501 gtagcgccaa atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca
    61561 gttcacccgt atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacgcgt
    61621 ctggcgtcag gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga
    61681 aggcgttttc ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc
    61741 agatattggc cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca
    61801 tcgagccagc cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt
    61861 ttttccaccg tctgtacaaa ggtgctggct tcaatcaggc tgtcgtgagt gccagtgtcc
    61921 agccaagcaa atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc
    61981 tggttgatgg aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac
    62041 tccacgactt tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt
    62101 tttggctttt cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag
    62161 cgttccgggt ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca
    62221 tgacgcagct tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa
    62281 gaaggttcac cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg
    62341 ctgggctgtt cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc
    62401 tggaaataac ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc
    62461 atcagcaccg acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc
    62521 gatacgccgc gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct
    62581 ttcatgtttg cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg
    62641 ccgtcctgca cctgcttcca ccaactttca ttagccagat accactgcac cgttttacgc
    62701 attccacttt caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca
    62761 tcaatggcat agcgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga
    62821 taatgcacca cgccttgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt
    62881 tccacaacat cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact
    62941 ttcccggtgg tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca
    63001 cggatttgct gcccgttgcc atataccggc agcgatttac ccgccagcgc gttgaggatc
    63061 atcagcggga tcagtttttc cggaaagtgg taagggccgt agttattcga gcagttggtg
    63121 ataagcgtcg gcagaccata ggtccgcagc caggcgcgca ccagatggtc gctgctggct
    63181 tttgacgcgg aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc
    63241 gtcgagtgca ggtcaccata tacttcgtca gtggagatat gatgaaaacg gaacgctgat
    63301 tttttatctt ccgttagcgc attccagtaa gcccgcgccg cttcaagcaa tgtataagtc
    63361 ccgacaatgt tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt
    63421 tcggctgcca gatgcatgac acagtctggc tgatgctcag tgaatacgcg cgccagttct
    63481 gcccgatcgc agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc
    63541 agcgacatca ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc gtcgctcgtt
    63601 tcgttgatga tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga
    63661 atctttctca tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt
    63721 cgccattgat aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg
    63781 ccagcgcctc gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt
    63841 ggatgttagg ctcaacaacc agagtttcgc cgctatgcca ctgggcgatc agttcagcga
    63901 tttccatcgc cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc
    63961 aggcgatttt cagttcactg gcgcgtttat cggtagccgc caggcaatca gccaccgccg
    64021 ctttcacctg atcgataacc cagaacggtt tgtgatcgtt cacttcgcgc gcggtacgga
    64081 taagccgcgc ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt
    64141 gaccgcccac gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc
    64201 gaatcagttc ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa
    64261 aagcgatatt cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg
    64321 agttagtgac gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggccg
    64381 aacaaaccgg cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct
    64441 ggcccggtaa cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct
    64501 gctgtgggaa agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg
    64561 accccaccgg cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa
    64621 tggagcgagc agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg
    64681 tgggtacagc aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac
    64741 cgccttctac ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc
    64801 cacgattgat agtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc
    64861 gtgaggcaaa cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg
    64921 tcgcaaaact catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc
    64981 ctgaccatca ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt
    65041 taaaagacgc gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt
    65101 acccgccgtc accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc
    65161 cagcgaaggc gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt
    65221 catcagccag acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg
    65281 ccccagaatg cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat
    65341 ctggatgtcc tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc
    65401 acgaccgaag ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat
    65461 aaacgggtaa tttgccgcaa gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg
    65521 cacccataac agtgcatcaa tgactgtatt accggtaatg aagattcggc tatctgcaac
    65581 gttttcacgc agcaagtttt gccgggaagt ttcggttgga gagaaatgat acatcgccag
    65641 atgcccggtc aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg
    65701 taaaccagcc tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc
    65761 cagcgtcgtc gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat
    65821 aggttttagc ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat
    65881 tatgttgaga tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc
    65941 ccgatgctgc gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg
    66001 caccaacgga gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac
    66061 atcgattctc ttcgaataag cggcgagcga ctttgcgctc accgcagcag tgttgctatt
    66121 tcgagcaacg gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca
    66181 taatcatcag gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca
    66241 aatagcgata ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca
    66301 tggcccgatt ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca
    66361 gtcgagcctg gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg
    66421 caggcacatc tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct
    66481 caatgctgtt catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct
    66541 gagctttcat ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc
    66601 tggctgcacg ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg
    66661 cggtttcggc aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa
    66721 actggatgtt gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca
    66781 tccgctgctt gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca
    66841 gttgcataac aaactcttta taggcttcgt ccatgaccga tggttggtcg gcagaagcca
    66901 tgtttgaacg gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca
    66961 tattcaccgt tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaacg
    67021 tatacgccag cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag
    67081 cccacaaggt acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt
    67141 tcccaggcat tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc
    67201 acgatttcta cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg
    67261 cttaatgcaa tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc
    67321 cgggacaaaa tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag
    67381 ggtaatcagc acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat
    67441 atgctgccgg tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc
    67501 caccatatcc attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg
    67561 ggttttgccc tgggtcgttt cgagcaggat ccagatcacg gtaaaaccaa tcagcgtact
    67621 gcccgcatca cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat
    67681 gtatggcagg atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc
    67741 gaaccacaaa atcataccga ttgctgcaaa cgagacacag gacaacccgc ccagcaagcc
    67801 atcaatgcca tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag
    67861 gaagtaacca aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact
    67921 gagataaagc ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg
    67981 gatttttacg ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc
    68041 acaagcgaga tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca
    68101 aatccctgcg taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg
    68161 gaagtttggt ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa
    68221 cagtgtcgtg aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat
    68281 gcatgctctc agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg
    68341 gaaagtccgt tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg
    68401 cataacatgc cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta
    68461 cagatataaa agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat
    68521 gagcgtttca tcatttcgaa gaaatcgtca ttggtcttgg tcattgccag tttattaatg
    68581 aggaattcca ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt
    68641 ttctgcagtt cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag
    68701 tcgatagccg ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg
    68761 cctgtacctt taaactcttc gtagataact tcgtccattt tagaaccagt atcgataagc
    68821 gccgtcgcga taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag
    68881 cgtttcggac gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc
    68941 ggaacaacgg tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg
    69001 tctttcttgt gctcaaccag gcgtttcgcc ttctcgatca ccatttccgc aacctgaacg
    69061 tggcgagatg cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc
    69121 atctcggtta cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg
    69181 tggttgtaag caatgctctg agcaatgttc tgcagcagca tggttttacc ggctttcggc
    69241 ggtgccacaa tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga
    69301 gcagttaaat cttccgtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc
    69361 agcggggtta agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta
    69421 acttcgttaa ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta
    69481 ccagagatgg tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag
    69541 atgtcatcag gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc
    69601 tgcaatatct ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc
    69661 aggatggcaa aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt
    69721 tcgccgagag tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg
    69781 gtgtgagttc ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga
    69841 tcatccatgc ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag
    69901 tacgcagaac tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc
    69961 atttaagata aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt
    70021 aatacgaagt caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg
    70081 ggcacccgaa catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac
    70141 ctttagacag tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag
    70201 tcgggatacc acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt
    70261 ttgcaacggt cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca
    70321 ttttgcacgg accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga
    70381 gtacatccgt gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact
    70441 ccacaggaat aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg
    70501 ttgactttat ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca
    70561 ctatgagcaa aacacattta acagaacaga agttttccga cttcgccctg catccgaagg
    70621 ttgtagaagc ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc
    70681 ttccgctgac gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa
    70741 cgatggcgtt tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc
    70801 gcaaggtgaa tcagccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga
    70861 tccatgccga tgcggaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg
    70921 gtggtgatgg ttacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg
    70981 gcaccacggg tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc
    71041 aggtagtggt actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc
    71101 gctggctgtt ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca
    71161 cgctttcgta ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg
    71221 aagtggaacc ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta
    71281 acgaagaaaa aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga
    71341 ttattttcgc caacaccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg
    71401 gtcatcgtgt cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg
    71461 atgaatttac ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt
    71521 tgcatattcc ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt
    71581 acgttcaccg tattggtcgt acaggtcgcg caggcgcaag cggtcactct attagcctgg
    71641 cgtgtgaaga gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc
    71701 cggtaagcaa atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca
    71761 cgcgcccgcg tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt
    71821 caggttaaat aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat
    71881 gaatcctgaa tcacttactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag
    71941 acagattgaa ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc
    72001 ggttcgaata gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg
    72061 acgcgaataa aacgcaaagt gcgtctggct gctggcctga atagtgaaaa tgccctctct
    72121 aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat
    72181 attcctccct cgcaaattcg cgttgtcgct acggcgacgt tacgccttgc cgtcaatgcg
    72241 ggtgatttta ttgccaaagc gcaggaaatt ctcggttgtc cggtacaggt gatcagcggt
    72301 gaagaggaag cacgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag
    72361 cgcctggtgg tggatatagg cggagccagt actgaactgg taaccggcac gggtgcacaa
    72421 actacctcgt tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc
    72481 gatcgtaatc tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta
    72541 cgtccggttg ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc
    72601 accgtgcagg cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg
    72661 gaaaagttgc agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag
    72721 attgacgggc tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc
    72781 gccattttta ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa
    72841 ggcctggtct acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg
    72901 cgtaatattc aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt
    72961 gcggctaact tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat
    73021 ttgctcatca gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagcg
    73081 ccgcaacacg ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag
    73141 aaaaaattgc tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg
    73201 catcagcaaa atgccgtacc gccgcgcgtc gcagaacaac tctgccgttt actacgcctg
    73261 gccatcattt ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct
    73321 aaccatgaac tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt
    73381 aaagagatta ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg
    73441 cattaatgag taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc
    73501 ctcgttttgc cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc
    73561 gttcttcggc gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct
    73621 ccagcaaaaa gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac
    73681 acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct
    73741 cctcatcgat attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac
    73801 cgaccatgta gacataagga aactccagcc ctttcgacgc gtggagagtc atcagttgca
    73861 cctgatccag ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg
    73921 tcaccacctg ggttaacgtc atcggctcat ccagttcact gccttcgagc atctccgtca
    73981 tccagctaaa cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg
    74041 atgtttcgta cagccaggat tcataatcca tgccgtggat cagatcacgc accgcggcaa
    74101 tcggctcccg ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg
    74161 cttcataacc acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca
    74221 tgcttttatt gcgcgtcatc gcccattcac ccaacttttt tagcgtcgcc gggccaatct
    74281 ctcgcttcgg cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca
    74341 cgcgcagata agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag
    74401 aaattttgta cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt
    74461 taccgcgata aagaatggcg taatctttgt actgcgtttt attgacgaag tgatgggcga
    74521 tcagctcgcc agtaacgcgc tcggcttcat gttcctcgtt attcgcgctt aaaactttta
    74581 gctctgtgcc gtaacccagt tcggagaaca gacgcttttc aaagacgtgc ggattattgg
    74641 cgatcaggat gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct
    74701 taatcacctt cagcgccgga aaatcctgac tcagcagcac caggttttgt ggacgtgcgc
    74761 cgcgccagga gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc
    74821 ccaccagcag tttcaccagc tcatactggc tggtgttggt gtcctgatac tcatccacca
    74881 gcagatagcg aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca
    74941 acgtcggcaa taaaatcaga tcatcgaagt cgagaacgtt acaggctttc aggtgtgcat
    75001 catacagccc ataacaatgg gcaaaaatac gatcccgctc gccctttgcc tctgccgccg
    75061 cctgcgccgg tgttttgaga tcattcttcc agttagagat ggtcgaaatc agttgttgca
    75121 ggagaacttt gtcatcttca atcagcccct cggtcaactc tttaagcaaa gcaagctgat
    75181 cggtatcgtc aaacaacgag aagttcgctt tcatcccaag cgccgcatac tcgcgtttga
    75241 tgatatccag ccccaacgta tggaaagtgg agatcatcag cccacgcgcc tctttgcgcc
    75301 ccagcgtctg ccctacacgc tctttcatct cgcgcgctgc tttattagta aaggtcaccg
    75361 ccgcaatgtg ccgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga
    75421 taacacgagt tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt
    75481 cgacagcttg ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg
    75541 ggggaggatt gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg
    75601 gctgcggatg tttaaatcag agagagaaga ccgaaatcgg agttcatcgt gaatccatta
    75661 gacttagaaa atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag
    75721 aggtttactc gtggcgatta atttcagccc taaggttggt gaaatactgg aatgcaactt
    75781 tggtaactat cctgttagcc aaaatggacc gttcatcacc acgtattacg atggccgcat
    75841 tcctcctgaa atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa
    75901 cgcatgtatc gtcgttccat tatcaaccac tcgcgaccat gacaagttaa atcgtggaat
    75961 gcatgttgaa atagccagta atattatcaa tgacctgcaa ttctttgatc agcagattcg
    76021 ttgggccaaa gccgacctgg tacaacaagt cagtcgaaac agattaaaca gagcaagaac
    76081 ttacagaggg tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc
    76141 ggtcatcaag tcaatcaatg ccatatcgct cataaattga caataaaatc accatccccg
    76201 ataatgactc tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc
    76261 ccggattttc cgggcttttg ccatatatgt gacccgtcct gactgacaat tacgttagca
    76321 tctgcggatc atgctaaaat cgccgcctga caattattca ctcaaggcac gatcatggca
    76381 aaaacagcag cagcactgca tatccttgta aaagaagaga aactggctct ggatcttctc
    76441 gagcagatta agaacggggc cgattttggc aagctggcga agaaacactc catttgccca
    76501 tcaggcaaac gcggcggtga tttaggtgaa ttccgccagg gtcagatggt tccggcgttc
    76561 gataaagttg tgttctcttg tccggtactg gagccgactg gcccgctgca tacccagttc
    76621 ggttaccaca tcattaaggt gctttaccgt aactaacagt aaggccttct ccaggagaag
    76681 gccttgaact ctgaatcgct attaacccgc aacagcaatg cgtttcatat ctgtcatata
    76741 gccgcgcagt ttcttaccta cctgctcaat cgcatggctg cgaatcgctt cgttcacatc
    76801 acgcagttgc gcgttatcta ccgcgccttc cggaatagct ttacccaggt cgcccggttg
    76861 cagctctgcc ataaacggtt tcagcaacgg cacacaagcg taagagaaca gatagttacc
    76921 gtactcagcg gtatcagaga taaccacgtt catttcgtac agacgcttac gggcgatggt
    76981 gttggcaatc agcggcagct cgtgcagtga ttcataatat gcagactctt caatgatgcc
    77041 ggaatcgacc atggtttcga acgccagttc aacgcccgct ttcaccatcg cgatcatcag
    77101 tacgccttta tcgaagtatt cctgctcgcc gattttgcct tcatactgcg gcgcggtttc
    77161 aaacgcggtt ttgccggttt cttcacgcca ggtcagcagt ttcttatcat cgttggccca
    77221 gtccgccatc ataccggaag agaattcgcc ggagatgatg tcatccatat gtttctggaa
    77281 cagcggtgcc atgatctctt tcagctgttc agacagcgcg taagcacgca gtttcgccgg
    77341 gttagagaga cggtccatca tcagggtgat gccgccctgt ttcagcgctt cggtgatggt
    77401 ttcccagccg aactgaatca gtttttctgc gtatgccggg tcggtgcctt cttccaccag
    77461 cttgtcgaag cacagcagcg agccagcctg caacataccg cacagaatgg tttgctcgcc
    77521 catcaggtca gatttcactt ccgcaacgaa ggacgattcc agcacgcccg cacggtgacc
    77581 gccggtcgct gccgcccagg ctttggcaat cgccatgcct tcaccttttg gatcgttttc
    77641 cgggtgaacg gcaatcagcg tcggtacacc gaatccacgt ttgtactctt cacgtacttc
    77701 ggtgccaggg catttcggcg caaccattac tacggtgatg tctttacgga tctgctcgcc
    77761 cacttctacg atgttgaaac catgcgagta acccagcgcc gcgccgtctt tcatcagtgg
    77821 ctgtacggtg cgcactacat cagagtgctg cttgtccggc gtcaagttaa ccaccagatc
    77881 cgcctgcggg atcagttctt cgtaagtacc aactttaaag ccgttttcgg tcgctttacg
    77941 ccaggacgcg cgcttctcgg caatcgcttc cttacgcagc gcgtaggaga tatccagacc
    78001 agaatcacgc atgttcaggc cctggttgag accctgtgcg ccacagccga cgatgaccac
    78061 ttttttaccc tgaaggtagc tcgcgccgtc agcgaattcg tcgcgaccca taaagcgaca
    78121 tttgcccagc tgtgccagct gctggcgcag gttcagtgta ttgaagtagt tagccatggt
    78181 gattcctcgt gatgttgtgc ttcttattgt tcggttcgct tttgcgagat tgaattcact
    78241 atatgacagg aaatttattg cggaaattga tatattcaca acgtcacatt gcaatttttg
    78301 caacgtcaac atcgagggct gtccctgtgg atttacgcga tctgaaaacc ttcctgcatc
    78361 tggcggaaag ccgccatttt ggccgcagcg cgcgggcgat gcacgttagc ccatccacgc
    78421 tctcacggca gattcagcgc ctggaagaag atctcggtca gccgctgttt gtgcgcgata
    78481 accgcacggt gacgctgact gaagcgggcg aagaactgcg cgttttcgcc cagcaaacgc
    78541 tgttgcaata tcagcaattg cgccacacca tcgaccagca aggaccgtcg ctctctggcg
    78601 aattacatat cttctgctcg gtgaccgctg cctacagcca tctgccgccg attctggatc
    78661 gcttccgcgc ggaacatccg tcggtggaga tcaaactcac cactggtgat gcggcagatg
    78721 caatggaaaa agtggtcacg ggagaagcgg atctggcgat tgccgggaaa ccggagacgc
    78781 tacccggcgc tgtggcgttt tcgatgctgg agaatctggc ggtagtgctg attgcgcccg
    78841 cgctgccctg cccggtgcgt aatcaggtct cggtagagaa gccggactgg tcaacggtac
    78901 cgtttatcat ggccgatcag gggccagtac gccgccgcat tgaactgtgg tttcgacgca
    78961 ataaaatcag caacccaatg atttacgcca cggttggcgg gcatgaagcg atggtatcga
    79021 tggtggcgct cggctgtggt gtggcgctgt tgccggaagt ggtgctggaa aacagcccgg
    79081 agcctgtacg caaccgggta atgattttag atcgcagcga tgagaaaacg ccgtttgaac
    79141 tgggcgtgtg tgcacaaaaa aagcggctgc atgagccgct gattgaggcg ttctggagga
    79201 ttttgccgaa ccacaaatga cgttgtcgcg cgagtaggcc tgataagcga agcgctatca
    79261 ggcatttttc cctaacccgc caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg
    79321 caatcgtagc ccagctcatt tagccgggtt tcgaaatccg gttcatggtc gccaagctcg
    79381 aacgccgcca gtacacgccc gtagtcggta ccgtgactgc gataatggaa cagagaaata
    79441 ttccagtgcg tacccagcgt gttgaggaag cgcagcagcg cacccggcga ttccggaaat
    79501 tcgaagctgt agagacgttc ctgcaacgga tgcgatggac gccccccaac catatagcgc
    79561 acgtgtaact tcgccatttc gtcgtcggag agatcaacca cgctgtagcc gccgtcgttg
    79621 agcatctgca aaatttcttt gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag
    79681 atgcaggcgt ttttggcatc ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca
    79741 agcagttggc agaatttgag gaagctgcct ttttcttccg gaatggtcac cgccaacaac
    79801 gcttcacgct gttcgcccag ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc
    79861 acgttggcac cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata
    79921 tattttttca ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc gcgcacatct
    79981 tcgaataaat ccttcatcgc cgcacagatc gcatcactat cgacggtgat gatatcgtcg
    80041 agatactcct ggcacaaacg gaaggtttcg tcgccgatgc gttttaccgc aacgccttca
    80101 gcaaacagcc ccacgcgcgg cagatcaacc ggatgacccg catccagcgc tgctttcagg
    80161 caggcggagt cttccgcttc tacggcgatc actttgattt gcggcatcag ttgtttgatc
    80221 agcaccgcca cgcccgcagc cagaccgccg ccgccgactg gcacaaatac gcggtcgaga
    80281 tgggcgtcct gctggagcag ttccagcgcc agcgtgcctt gcccggcaat caccatcgga
    80341 tgatcgaacg gcggcaccca ggtgaacccc tgctgctgtg acagttcgat cgctttggct
    80401 ttcgcttcat caaagttcgc gccgtggagc agcacttcgc cgccgaagcc gcgcaccgcg
    80461 tcgactttga tatcggcggt ggcggttggc atgacgatca gcgccttcac gcctaaccgt
    80521 gcggaagaaa acgcgacgcc ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc
    80581 gctttttgtt cttccgtcag gcccgccatc atggcgtatg cgccgcgcag cttaaagctg
    80641 tgcactggct ggcgatcttc gcgcttcacc agaatgacgt tatcaagacg cgacgacagt
    80701 ttttccattt tttgtagcgg cgtaacctgc gccgcctcgt aaaccggcgc gcgcagcact
    80761 gctcttaaat attcggcacc ttccggagca ccggacaggg gttgcgagtc agccattatt
    80821 aaccccccag tttcgattta tcgcgcaccg cgcctttgtc ggcgctggtt gccaggctgg
    80881 cataagcacg cagggcaaag gagacttgac gttcacgatt tttcggcgtc caggctttgt
    80941 caccccgagc ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt acctgtaact
    81001 gaatgccacg gttcggaatg tcgatggcga tcaggtcgcc gtcttcaatc aggccaatgc
    81061 tgccgccgct tgccgcttcc ggtgagacgt ggccgataga aaggccagag gtgccgccag
    81121 agaaacgacc gtcggtgatc agcgcacagg ctttgccgag gcccattgat ttcaggaagc
    81181 tggttgggta gagcatttcc tgcatgccag ggccgccttt cggaccttca tagcggatca
    81241 ccaccacatc gcccgcgaca accttgccgc cgagaatcgc ttctaccgcg tcgtcctggc
    81301 tttcgtatac tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt
    81361 taacgatgca gccgttttct gcgaaattac cgtagagcac tgccaggccg ccgtctttgc
    81421 tgtacgcatg ctccagcgag cgaatacagc catttgcgcg atcgtcgtcc agcgtatccc
    81481 aacggcaatc ctgcgagaat gcctgtgtag tacgaatgcc cgccgggcct gcacggaaca
    81541 tattttttac cgcgtcatcc tgggtcagca taacgtcgta ttgttccagc gtttgcggca
    81601 acgtcaggcc aagtacgttt ttcacatcac ggttcagtaa ccccgcacga tccagctcgc
    81661 cgagaatacc gataacacca ccagcacggt gaacatcttc catatggtat ttttgggtgc
    81721 tcggcgcaac tttgcataac tgcgggactt tacgcgaaag tttgtcgata tcactcatgg
    81781 tgaagtcgat ttccgcttcc tgcgccgccg ccagcaggtg aagtacggtg ttagtcgatc
    81841 cacccatcgc gatatccagc gtcatggcat tttcaaacgc cgctttgcta gcaatattgc
    81901 gaggcagtgc actttcgtcg ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt
    81961 taccagcatt gaggaacagc tgcttacggt cggcgtgagt tgccagcagc gagccgttgc
    82021 ccggctgcga caggcccagc gcttcggtca ggcagttcat tgagttagcg gtaaacatcc
    82081 cggagcagga accgcaggtt gggcatgcgg aacgttcaac ctgatcgctc tgggagtcag
    82141 aaactttcgg gtctgccccc tggatcatcg catcaaccag atcgagcttg atgatctgat
    82201 cggacagttt ggttttcccg gcctccatcg ggccgccgga aacaaagatc accggaatat
    82261 tcaggcgcag agaggccatc agcatccccg gggtgatttt gtcacagtta gagatgcaga
    82321 ccatggcgtc ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg atcagttcgc
    82381 gagatggcag tgaataaagc atccccccat ggcccatggc aatcccatca tccaccgcaa
    82441 tggtgttgaa ctctttggca acgccgccag ccgcttcaat ttgttcggcg accagtttac
    82501 cgagatcgcg cagatggacg tgacccggta caaattgggt gaacgagttc acaaccgcga
    82561 taatcggctt accgaaatcg gcgtcggtca ttccggtggc gcgccacagc gcacgagcac
    82621 ccgccatatt acgaccatga gtggtggtgg cggaacggta cttaggcata ctttatttac
    82681 tcccagtgtc tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg tatttattga
    82741 ttaacttgat ctaaccagcc ccatttatct tcggtttcgc cggtgaagag gccgaagaag
    82801 gcttgctgaa tacgtttggt aaccgggcca caacggcctt cgccaacctg aataccgtct
    82861 acgctgcgca ctggcgtgat ttctgccgcc gtaccggaca taaacacttc atccgccagg
    82921 tacagggatt cgcgcgacag tacctgctca cgcacttcaa ttcccagctc tttcgccagt
    82981 ttgatgatgg catcacgggt aatacccggc agcgcggagg aggtgaacgg tggggtgaac
    83041 agcacaccat ctttcacttc aaacaggttt tcgcctgcgc cttcagagat gtaaccattc
    83101 acatccagcg cgataccttc ctgataaccg tggcggcgtg cttcgctacc caccagcagg
    83161 gaagagagat agttaccacc ggctttcgcc gcggttggga tggtgtttgg tgctgcacgg
    83221 ttccaggagg aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc cagatacgct
    83281 ccccacggga aagcggcgat aatcacatcg gttgagtatc ccgctggcgg gttaacgccc
    83341 ataccaacat caccgacgaa gatcagcgga cggatatagg cgctggtgag attgtttttg
    83401 cggatcacgt cacgacaagc ttccatcagc tcatcaatgc tctgcgaaac cgggaagcga
    83461 tagattttgg cggagtcatg cagacgctgc atatgctcac gatggcggaa tacaaccggt
    83521 cctttgtgcg agtcgtagca acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg
    83581 tgcgacatca catgcacctt cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg
    83641 taatcagctt tcttcgtggt catttttata ttccttttgc gctcaggcgc ggatttgttg
    83701 tgatgtggtt gtgctctggc agatggcaac gtgtgcgacg tccaccagtt tatttaactg
    83761 actaaacagt aagtcgaccg accgtgggct ggcaacggtc aattcgatat ttatattttg
    83821 tgcatcgctg gcggcggcca tattcattga gcagacgtgg aaaccacgat gacgcaccac
    83881 gcgtaaaaca cgttctaagg tttctggatt gaagcgagcc gatacattga cctgatgttg
    83941 catcatgata atttctccaa catttctgaa ttactggcgc caggcggcac cagcggccag
    84001 acgttctcaa gttcgtcgat tgagacatga agcaggtatg gcccatcact gttcagcatg
    84061 gtgtcgagtg ccgcttcaac ctggtcttta cgggtgatgt gttggccagg gatgccgaag
    84121 gcgctggcta acatgaggaa atcggggtta tcagtaaggg tggtttcgct gtatcgttcc
    84181 tgaaaaaaca gttgctgcca ttgtcgaacc atccctaacc gttggttatc gagtaagacg
    84241 attttcaacg gtaactgctt gcgttttacg gtgcccagct cttgcacatt catcatgaaa
    84301 gagccgtcac cggagataca gacaacggta tcgttcggtc gcgcgacttg tgcgccaacc
    84361 gccgccggta aaccaaaacc catggtgcct aagccgctgg aggtaatgaa attttccggg
    84421 cgagtgtgtg cgatgtgctg cgcggcccac atctgatgct gccccacatc tgtggtcaca
    84481 acgcaatccg caggtttacg atccgacagt tgttttaaca acaacggcgc gtagatagtg
    84541 tcaccgggat ggtcgtaacg ccaggcatgt tcatcactca gctgcgcgca gtattgctgc
    84601 cagtcattga tatttaatgg ctgctgtaat gctggtaaca gcgcatttaa atcaccctgt
    84661 aatgccacat gtgcctgacg cagcttgttc atttctgccg ggtcgatatc catatggata
    84721 acactggcgt gtggtgcgaa ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca
    84781 cccacggcga tcagcaagtc gcactcctgc accgcgaagt tcgccgcttt ggtgccgtgc
    84841 attcccagca ttcccagata gtacgaataa tctgctgcaa ctgcgcccag ccctttcagc
    84901 gtgcaggtgg caggcatttt tgtggtagag agaaattctc gtaaagcagg aactgcctgc
    84961 gccataccca cgccaccacc aacgtacagc atcggttttt gcgcttttgc cagcatctgg
    85021 cgcgcttgct cgacttcggc atgggggaaa gtcacttcgt tttcaacggt ggtgaaccac
    85081 ggttccaggt cgccgctggc taactggata tcttttggga tatcgaccag aaccggacca
    85141 ggacgacctg agctggcaac gtcgaatgct tcagccatga tgcgcggcaa ctcttccagc
    85201 gactgcacca ggaagctgtg cttagtacag gctaacgaca atcccaggac atccacttcc
    85261 tgaaatgcgt cagtgccgat aaacggtgcg gacacttgac cggtgatggc aacaacaggg
    85321 atagaatcta acagtgcgtc cgcaagcccg gttatcaggt tggttgctcc cggaccagac
    85381 gtggcgatac atacgccagt tttgccggta gcacgagcat aaccgatagc cgccattgcc
    85441 gcgccctgtt catgtcggca cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa
    85501 accggcataa ttgcgccacc cggataaccg aaaacggtgt tcacaccctg tgcccgcaac
    85561 gcatgtacca cccactgtgc gccattcata gttagttccc cgtcctgaat cttgagaaac
    85621 agaattttgt gctgttattc attgtctgct cctcggttat gtttttaagg tcaaaaaaaa
    85681 cccccggacc tttcggtgcg ggggtcttag ttcgttaagg cttgatctct aagcctttcc
    85741 tcgtccaagt gcagccccgc acggtgggat aataatcacc accacgctaa tcacgaccag
    85801 gctaatcact cgtagaaggg ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg
    85861 cctaaagagt taccatagat tttgcgaata gcacaagata ttttttggcc gatttttatg
    85921 atgcctttaa taatttcaat agaaattgtt gttttttaaa aagaaaatag tgagtgaaaa
    85981 aaattcaatt tcagtataaa tgttgttccc ttcctcgtag ttcatccttt gtccgctgca
    86041 tttttgcgag catcattcca ccgccagaac aactcaactt cacttgcaca aataaacagg
    86101 aatcagccac taaaattcac gaattagcgg gctacagcag cgatatctta tgcacataat
    86161 ggtgactcaa ggagggctta tgtcactgtc aattgttcat acccgcgcag ccctgggagt
    86221 aaatgcgccc ccaatcactg ttgaggtaca tatcagtaaa ggtctacccg gcttaacgat
    86281 ggtgggctta ccagaaacaa cggtaaaaga agcccgcgat cgcgtgcgca gcgccattat
    86341 caatagcgga tatgaatatc cggccaaaaa aatcaccatc aacctagctc cagccgatct
    86401 gccaaaagaa gggggacgat atgatttacc tatcgccatt gcgttgctgg cggcctcaga
    86461 acagcttaca gccaataagt tagatgaata tgaattagtc ggagaactgg cgcttacagg
    86521 cgctctgcgt ggcgttcccg gtgcaatctc cagtgcaact gaagctatta agtcgggcag
    86581 aaaaattatc gtcgcgaaag ataacgaaga tgaagtgggg ctaattaacg gtgaaggatg
    86641 cctgatagcc aatcatctgc aggctgtctg tgcgtttctg gaaggtaagc acactctcga
    86701 acgcccgaaa ccaactgatg cagtatcccg ggcgctacaa catgatctca gtgatgttgt
    86761 cggtcaggag caaggaaagc gaggactgga aattaccgcc gctggcgggc acaacctttt
    86821 actgatcgga ccgccgggaa caggtaaaac aatgctcgcc agccgtatta atggtctttt
    86881 gccagattta agcaatgaag aggcactgga gagcgctgcg atattaagtc tggtaaatgc
    86941 tgaatcagta caaaaacaat ggcggcagcg cccgttccgc tcacctcatc acagtgcgtc
    87001 gttaactgcg atggtgggcg gtggtgcaat tccagggcct ggtgaaattt cgttggcaca
    87061 taacggcgtg ctttttcttg atgagctacc tgaatttgaa cggcgtacac tggatgcttt
    87121 gcgagagccg attgaatccg ggcagatcca tctttcacgc acacgagcaa aaataaccta
    87181 tccagcccgt ttccagcttg ttgcggcgat gaatcccagc cctaccggac attatcaggg
    87241 aaaccataac cgctgcacgc cagaacagac attacgttat ctcaaccggc tctcggggcc
    87301 ctttctcgac cgcttcgatc tctcactgga gatcccatta ccaccccccg gcattttgag
    87361 taaaacggta gtgccgggag aaagcagcac caccgttaaa caacgtgtaa tggctgccag
    87421 agagcgccaa tttaagcggc agaataaact gaatgcctgg ctggatagtc cggaaatacg
    87481 caaattctgc aagcttgaga gcgaagatgc gcagtggctg gaagaaacgc tgatccatct
    87541 ggggttatcg attcgtgcct ggcagcggtt attgaaagtt gcacgaacca ttgctgatat
    87601 tgatcagtct aacattatca cacgtcagca tttgcaggag gcagttagct atcgtgcgat
    87661 tgaccgtttg ctcatccatc tgcaaaaact actgacataa aaaaagggca tttcgccctt
    87721 tttattaatc gtcagaatcg gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg
    87781 aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat atacttggac cacacgcgtt
    87841 ctgcttctgt cactggctca cgttcgccac ggcatactgc tacgaagagt ttctcttcct
    87901 cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa ggcataacca tgacgctcaa
    87961 gcagttgtgc ctctttgatg gtgaaatcac catgacgaga gaatccacgt ggataatgtt
    88021 tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc catcctgcac gctcctaatt
    88081 ctttgaccga gctagttatg gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa
    88141 acatttaaat cataacgaca aataattttg cggagagcac tgtggatacg gaattgttaa
    88201 aaactttcct ggaagttagc cgaacgcgtc actttggtcg agcggctgaa tcgctctatc
    88261 tgacccagtc agcagtgagc tttcgaatca gacaactgga aaatcaactg ggtgtgaacc
    88321 ttttcacccg ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa ctactgcctt
    88381 atgcagaaac gctcatgagc acctggcagg ccgcccgtaa ggaggtggcg catacctcac
    88441 gacataacga gttttctatc ggtgccagcg cctcattgtg ggaatgtatg cttaatcagt
    88501 ggctgggacg tttgtatcaa aatcaggatg cccatacagg cttacagttc gaagcgcgaa
    88561 ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat cttcttatta
    88621 ccactgaagc gcccaaaatg gacgaattta gtagtcagtt gctggggtat ttcactttag
    88681 cgctttatac cagtgcccct tccaaactaa agggagatct taactatctg cgactggagt
    88741 gggggccaga ttttcaacag catgaggcag gtttgatcgg tgctgacgaa gttcccattc
    88801 tgacaaccag ttctgctgaa ctggcacagc aacagattgc gatgcttaat ggttgcacct
    88861 ggctacccgt cagctgggcg cgtaaaaaag gcggcctaca caccgttgtc gatagcacaa
    88921 cactttcgcg gccgctttat gccatatggc tgcaaaatag cgataaaaat acgctgattc
    88981 gcgatctatt gaaaattaac gtgctggatg aagtgtatta atctgaatgg ctggcaagga
    89041 tgccggtaga aggatttact tcagagaggg ttatttcaga taaaaaaaat ccttagcttt
    89101 cgctaaggat gatttctggc aggggcggag agactcgaac tcccaacacc cggttttgga
    89161 gaccggtgct ctaccaattg aactacgccc ctaattag
//
LOCUS       DAZGBC010000003        80164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGBC010000003 DAZGBC010000000
VERSION     DAZGBC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.003"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..591
                     /gene="yraH"
                     /locus_tag="ACHK1J_000174"
     CDS             7..591
                     /gene="yraH"
                     /locus_tag="ACHK1J_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7909516.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            671..1366
                     /gene="yraI"
                     /locus_tag="ACHK1J_000175"
     CDS             671..1366
                     /gene="yraI"
                     /locus_tag="ACHK1J_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7909517.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGLVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1395..3911
                     /gene="yraJ"
                     /locus_tag="ACHK1J_000176"
     CDS             1395..3911
                     /gene="yraJ"
                     /locus_tag="ACHK1J_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7909518.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYSYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3922..5013
                     /gene="yraK"
                     /locus_tag="ACHK1J_000177"
     CDS             3922..5013
                     /gene="yraK"
                     /locus_tag="ACHK1J_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7909519.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5056..5916)
                     /gene="rsmI"
                     /locus_tag="ACHK1J_000178"
     CDS             complement(5056..5916)
                     /gene="rsmI"
                     /locus_tag="ACHK1J_000178"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO7909520.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5981..8017
                     /gene="lpoA"
                     /locus_tag="ACHK1J_000179"
     CDS             5981..8017
                     /gene="lpoA"
                     /locus_tag="ACHK1J_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO7909521.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7975..8370
                     /gene="yraN"
                     /locus_tag="ACHK1J_000180"
     CDS             7975..8370
                     /gene="yraN"
                     /locus_tag="ACHK1J_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO7909522.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8390..8980
                     /gene="diaA"
                     /locus_tag="ACHK1J_000181"
     CDS             8390..8980
                     /gene="diaA"
                     /locus_tag="ACHK1J_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO7909523.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8990..9565
                     /gene="dolP"
                     /locus_tag="ACHK1J_000182"
     CDS             8990..9565
                     /gene="dolP"
                     /locus_tag="ACHK1J_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7909524.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9679..10719)
                     /gene="yraQ"
                     /locus_tag="ACHK1J_000183"
     CDS             complement(9679..10719)
                     /gene="yraQ"
                     /locus_tag="ACHK1J_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7909525.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10792..11427)
                     /gene="yraR"
                     /locus_tag="ACHK1J_000184"
     CDS             complement(10792..11427)
                     /gene="yraR"
                     /locus_tag="ACHK1J_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO7909526.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11555..12073
                     /gene="yhbO"
                     /locus_tag="ACHK1J_000185"
     CDS             11555..12073
                     /gene="yhbO"
                     /locus_tag="ACHK1J_000185"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO7909527.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12053..12496)
                     /gene="yhbP"
                     /locus_tag="ACHK1J_000186"
     CDS             complement(12053..12496)
                     /gene="yhbP"
                     /locus_tag="ACHK1J_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO7909528.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12547..12849
                     /gene="yhbQ"
                     /locus_tag="ACHK1J_000187"
     CDS             12547..12849
                     /gene="yhbQ"
                     /locus_tag="ACHK1J_000187"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO7909529.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12836..13339)
                     /gene="yhbS"
                     /locus_tag="ACHK1J_000188"
     CDS             complement(12836..13339)
                     /gene="yhbS"
                     /locus_tag="ACHK1J_000188"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7909530.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13333..13857)
                     /gene="ubiT"
                     /locus_tag="ACHK1J_000189"
     CDS             complement(13333..13857)
                     /gene="ubiT"
                     /locus_tag="ACHK1J_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO7909531.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14066..15061
                     /gene="ubiU"
                     /locus_tag="ACHK1J_000190"
     CDS             14066..15061
                     /gene="ubiU"
                     /locus_tag="ACHK1J_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO7909532.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMASNIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15070..15948
                     /gene="ubiV"
                     /locus_tag="ACHK1J_000191"
     CDS             15070..15948
                     /gene="ubiV"
                     /locus_tag="ACHK1J_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO7909533.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMSVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16029..17036
                     /gene="yhbW"
                     /locus_tag="ACHK1J_000192"
     CDS             16029..17036
                     /gene="yhbW"
                     /locus_tag="ACHK1J_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO7909534.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17154..18398)
                     /gene="mtr"
                     /locus_tag="ACHK1J_000193"
     CDS             complement(17154..18398)
                     /gene="mtr"
                     /locus_tag="ACHK1J_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO7909535.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18552..20441)
                     /gene="deaD"
                     /locus_tag="ACHK1J_000194"
     CDS             complement(18552..20441)
                     /gene="deaD"
                     /locus_tag="ACHK1J_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO7909536.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20434..20514)
                     /gene="yrbN"
                     /locus_tag="ACHK1J_000195"
     CDS             complement(20434..20514)
                     /gene="yrbN"
                     /locus_tag="ACHK1J_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO7909537.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20621..21505)
                     /gene="nlpI"
                     /locus_tag="ACHK1J_000196"
     CDS             complement(20621..21505)
                     /gene="nlpI"
                     /locus_tag="ACHK1J_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO7909538.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21614..23749)
                     /gene="pnp"
                     /locus_tag="ACHK1J_000197"
     CDS             complement(21614..23749)
                     /gene="pnp"
                     /locus_tag="ACHK1J_000197"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO7909539.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23996..24265)
                     /gene="rpsO"
                     /locus_tag="ACHK1J_000198"
     CDS             complement(23996..24265)
                     /gene="rpsO"
                     /locus_tag="ACHK1J_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO7909540.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24414..25358)
                     /gene="truB"
                     /locus_tag="ACHK1J_000199"
     CDS             complement(24414..25358)
                     /gene="truB"
                     /locus_tag="ACHK1J_000199"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO7909541.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25358..25759)
                     /gene="rbfA"
                     /locus_tag="ACHK1J_000200"
     CDS             complement(25358..25759)
                     /gene="rbfA"
                     /locus_tag="ACHK1J_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO7909542.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25924..28596)
                     /gene="infB"
                     /locus_tag="ACHK1J_000201"
     CDS             complement(25924..28596)
                     /gene="infB"
                     /locus_tag="ACHK1J_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO7909543.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIESESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28621..30108)
                     /gene="nusA"
                     /locus_tag="ACHK1J_000202"
     CDS             complement(28621..30108)
                     /gene="nusA"
                     /locus_tag="ACHK1J_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO7909544.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30136..30588)
                     /gene="rimP"
                     /locus_tag="ACHK1J_000203"
     CDS             complement(30136..30588)
                     /gene="rimP"
                     /locus_tag="ACHK1J_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO7909545.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30795..30871)
                     /locus_tag="ACHK1J_000204"
     tRNA            complement(30795..30871)
                     /locus_tag="ACHK1J_000204"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30835..30837),aa:Met,seq:cat)
     gene            31219..32562
                     /gene="argG"
                     /locus_tag="ACHK1J_000205"
     CDS             31219..32562
                     /gene="argG"
                     /locus_tag="ACHK1J_000205"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO7909546.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVDNLENKGQ"
     gene            complement(32570..34195)
                     /gene="yhbX"
                     /locus_tag="ACHK1J_000206"
     CDS             complement(32570..34195)
                     /gene="yhbX"
                     /locus_tag="ACHK1J_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7909547.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34654..34740)
                     /locus_tag="ACHK1J_000207"
     tRNA            complement(34654..34740)
                     /locus_tag="ACHK1J_000207"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34704..34706),aa:Leu,seq:gag)
     gene            complement(34755..35087)
                     /gene="secG"
                     /locus_tag="ACHK1J_000208"
     CDS             complement(34755..35087)
                     /gene="secG"
                     /locus_tag="ACHK1J_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO7909548.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35315..36652)
                     /gene="glmM"
                     /locus_tag="ACHK1J_000209"
     CDS             complement(35315..36652)
                     /gene="glmM"
                     /locus_tag="ACHK1J_000209"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO7909549.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36645..37493)
                     /gene="folP"
                     /locus_tag="ACHK1J_000210"
     CDS             complement(36645..37493)
                     /gene="folP"
                     /locus_tag="ACHK1J_000210"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO7909550.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37583..39517)
                     /gene="ftsH"
                     /locus_tag="ACHK1J_000211"
     CDS             complement(37583..39517)
                     /gene="ftsH"
                     /locus_tag="ACHK1J_000211"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO7909551.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39617..40246)
                     /gene="rlmE"
                     /locus_tag="ACHK1J_000212"
     CDS             complement(39617..40246)
                     /gene="rlmE"
                     /locus_tag="ACHK1J_000212"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO7909552.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40372..40665
                     /gene="yhbY"
                     /locus_tag="ACHK1J_000213"
     CDS             40372..40665
                     /gene="yhbY"
                     /locus_tag="ACHK1J_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO7909553.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40821..41297)
                     /gene="greA"
                     /locus_tag="ACHK1J_000214"
     CDS             complement(40821..41297)
                     /gene="greA"
                     /locus_tag="ACHK1J_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO7909554.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41545..42978
                     /gene="dacB"
                     /locus_tag="ACHK1J_000215"
     CDS             41545..42978
                     /gene="dacB"
                     /locus_tag="ACHK1J_000215"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7909555.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHTRFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43018..44190)
                     /gene="cgtA"
                     /locus_tag="ACHK1J_000216"
     CDS             complement(43018..44190)
                     /gene="cgtA"
                     /locus_tag="ACHK1J_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO7909556.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44206..45171)
                     /gene="yhbE"
                     /locus_tag="ACHK1J_000217"
     CDS             complement(44206..45171)
                     /gene="yhbE"
                     /locus_tag="ACHK1J_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7909557.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45298..45555)
                     /gene="rpmA"
                     /locus_tag="ACHK1J_000218"
     CDS             complement(45298..45555)
                     /gene="rpmA"
                     /locus_tag="ACHK1J_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO7909558.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45576..45887)
                     /gene="rplU"
                     /locus_tag="ACHK1J_000219"
     CDS             complement(45576..45887)
                     /gene="rplU"
                     /locus_tag="ACHK1J_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO7909559.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46146..47117
                     /gene="ispB"
                     /locus_tag="ACHK1J_000220"
     CDS             46146..47117
                     /gene="ispB"
                     /locus_tag="ACHK1J_000220"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO7909560.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPKEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47345..47623
                     /gene="sfsB"
                     /locus_tag="ACHK1J_000221"
     CDS             47345..47623
                     /gene="sfsB"
                     /locus_tag="ACHK1J_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO7909561.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47671..48930)
                     /gene="murA"
                     /locus_tag="ACHK1J_000222"
     CDS             complement(47671..48930)
                     /gene="murA"
                     /locus_tag="ACHK1J_000222"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO7909562.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48985..49239)
                     /gene="ibaG"
                     /locus_tag="ACHK1J_000223"
     CDS             complement(48985..49239)
                     /gene="ibaG"
                     /locus_tag="ACHK1J_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO7909563.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49399..49692)
                     /gene="mlaB"
                     /locus_tag="ACHK1J_000224"
     CDS             complement(49399..49692)
                     /gene="mlaB"
                     /locus_tag="ACHK1J_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO7909564.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49692..50327)
                     /gene="mlaC"
                     /locus_tag="ACHK1J_000225"
     CDS             complement(49692..50327)
                     /gene="mlaC"
                     /locus_tag="ACHK1J_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO7909565.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50346..50897)
                     /gene="mlaD"
                     /locus_tag="ACHK1J_000226"
     CDS             complement(50346..50897)
                     /gene="mlaD"
                     /locus_tag="ACHK1J_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO7909566.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50902..51684)
                     /gene="mlaE"
                     /locus_tag="ACHK1J_000227"
     CDS             complement(50902..51684)
                     /gene="mlaE"
                     /locus_tag="ACHK1J_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO7909567.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51692..52501)
                     /gene="mlaF"
                     /locus_tag="ACHK1J_000228"
     CDS             complement(51692..52501)
                     /gene="mlaF"
                     /locus_tag="ACHK1J_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO7909568.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAKALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52711..53688
                     /gene="yrbG"
                     /locus_tag="ACHK1J_000229"
     CDS             52711..53688
                     /gene="yrbG"
                     /locus_tag="ACHK1J_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO7909569.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53702..54688
                     /gene="kdsD"
                     /locus_tag="ACHK1J_000230"
     CDS             53702..54688
                     /gene="kdsD"
                     /locus_tag="ACHK1J_000230"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO7909570.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54709..55275
                     /gene="kdsC"
                     /locus_tag="ACHK1J_000231"
     CDS             54709..55275
                     /gene="kdsC"
                     /locus_tag="ACHK1J_000231"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO7909571.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55272..55847
                     /gene="lptC"
                     /locus_tag="ACHK1J_000232"
     CDS             55272..55847
                     /gene="lptC"
                     /locus_tag="ACHK1J_000232"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO7909572.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55816..56373
                     /gene="lptA"
                     /locus_tag="ACHK1J_000233"
     CDS             55816..56373
                     /gene="lptA"
                     /locus_tag="ACHK1J_000233"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO7909573.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56380..57105
                     /gene="lptB"
                     /locus_tag="ACHK1J_000234"
     CDS             56380..57105
                     /gene="lptB"
                     /locus_tag="ACHK1J_000234"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO7909574.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57153..58586
                     /gene="rpoN"
                     /locus_tag="ACHK1J_000235"
     CDS             57153..58586
                     /gene="rpoN"
                     /locus_tag="ACHK1J_000235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO7909575.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58609..58896
                     /gene="hpf"
                     /locus_tag="ACHK1J_000236"
     CDS             58609..58896
                     /gene="hpf"
                     /locus_tag="ACHK1J_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO7909576.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59014..59505
                     /gene="ptsN"
                     /locus_tag="ACHK1J_000237"
     CDS             59014..59505
                     /gene="ptsN"
                     /locus_tag="ACHK1J_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO7909577.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59551..60405
                     /gene="rapZ"
                     /locus_tag="ACHK1J_000238"
     CDS             59551..60405
                     /gene="rapZ"
                     /locus_tag="ACHK1J_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO7909578.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60402..60674
                     /gene="npr"
                     /locus_tag="ACHK1J_000239"
     CDS             60402..60674
                     /gene="npr"
                     /locus_tag="ACHK1J_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO7909579.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60888..61520
                     /gene="yrbL"
                     /locus_tag="ACHK1J_000240"
     CDS             60888..61520
                     /gene="yrbL"
                     /locus_tag="ACHK1J_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO7909580.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGERTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61517..62245)
                     /gene="mtgA"
                     /locus_tag="ACHK1J_000241"
     CDS             complement(61517..62245)
                     /gene="mtgA"
                     /locus_tag="ACHK1J_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO7909581.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPERWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62242..62895)
                     /gene="elbB"
                     /locus_tag="ACHK1J_000242"
     CDS             complement(62242..62895)
                     /gene="elbB"
                     /locus_tag="ACHK1J_000242"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO7909582.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63125..65461)
                     /gene="arcB"
                     /locus_tag="ACHK1J_000243"
     CDS             complement(63125..65461)
                     /gene="arcB"
                     /locus_tag="ACHK1J_000243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO7909583.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65557..66486)
                     /gene="yhcC"
                     /locus_tag="ACHK1J_000244"
     CDS             complement(65557..66486)
                     /gene="yhcC"
                     /locus_tag="ACHK1J_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO7909584.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            67161..71621
                     /gene="gltB"
                     /locus_tag="ACHK1J_000245"
     CDS             67161..71621
                     /gene="gltB"
                     /locus_tag="ACHK1J_000245"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO7909585.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIIETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSAFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71634..73052
                     /gene="gltD"
                     /locus_tag="ACHK1J_000246"
     CDS             71634..73052
                     /gene="gltD"
                     /locus_tag="ACHK1J_000246"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO7909586.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73236..74363
                     /gene="yhcG"
                     /locus_tag="ACHK1J_000247"
     CDS             73236..74363
                     /gene="yhcG"
                     /locus_tag="ACHK1J_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO7909587.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSLYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(74423..74887)
                     /gene="nanQ"
                     /locus_tag="ACHK1J_000248"
     CDS             complement(74423..74887)
                     /gene="nanQ"
                     /locus_tag="ACHK1J_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO7909588.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74884..75759)
                     /gene="nanK"
                     /locus_tag="ACHK1J_000249"
     CDS             complement(74884..75759)
                     /gene="nanK"
                     /locus_tag="ACHK1J_000249"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO7909589.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75756..76445)
                     /gene="nanE"
                     /locus_tag="ACHK1J_000250"
     CDS             complement(75756..76445)
                     /gene="nanE"
                     /locus_tag="ACHK1J_000250"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO7909590.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVEMLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76493..77983)
                     /gene="nanT"
                     /locus_tag="ACHK1J_000251"
     CDS             complement(76493..77983)
                     /gene="nanT"
                     /locus_tag="ACHK1J_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO7909591.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78092..78985)
                     /gene="nanA"
                     /locus_tag="ACHK1J_000252"
     CDS             complement(78092..78985)
                     /gene="nanA"
                     /locus_tag="ACHK1J_000252"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO7909592.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79107..79898)
                     /gene="nanR"
                     /locus_tag="ACHK1J_000253"
     CDS             complement(79107..79898)
                     /gene="nanR"
                     /locus_tag="ACHK1J_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO7909593.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19764 a        21685 c        19882 g        18833 t
ORIGIN      
        1 ttataaatga ataaagttac aaaaacagct attgcgggct tacttgcgct tttcgcaggt
       61 aatgccgctg caaccgatgg tgaaatagtt tttgatggcg agattttaaa atccgcttgt
      121 gaaatcaatg actctgataa gaaaattgaa gttgctcttg gtcactataa tgccgaacag
      181 tttcgttctg taggcgatcg tagcccaaaa attccattca ctattccttt agttaactgc
      241 ccagtgactg ggtgggagca cgataacggc aacgttgaag cgtctttccg tttatggctg
      301 gaaacgcggg ataatggtac cgtccctaac ttccctaact tggcaaaagt gggctctttt
      361 gctggcacag cagcaacagg tgtgggtatc cgtattgacg atgcggaaag cggaaacctt
      421 atgccactaa atgctatggg caatgataac acagtttatc agatccctgc ggactccgct
      481 ggaattgtca atgttgacct catcgcttac tacgtatcaa ccgttgaagc atccgaaatc
      541 accccagggg aagcagacgc tgtcgttaac gtaacgctgg attaccgtta atacgttacg
      601 gcgttatctg acctttcaga taacgccctt ttccttcctc tttctcgttg tatcaggttg
      661 aaaaataact atgtcaaaac gaacattcgc ggtgataata accttgttgt gtagcttctg
      721 tatcggccag gcgcttgcag ggggtatcgt tttacagcga acgcgagtga tctatgatgc
      781 cagccgcaaa gaggctgcgt tacctgtcgc aaacaaaggc gcagaaacgc cttatttact
      841 gcaatcatgg gtagataata tagatggtac aagccgtgcc ccatttatta taaccccacc
      901 gctatttcgt cttgaagctg gcgatgattc atcactgcga attattaaaa cagctgataa
      961 cctgcctgaa aataaagagt cgctgttcta cattaatgtt cgagccattc cagcaaagaa
     1021 aaaatcagat gatgttaatg ctaacgagtt gacgctggta tttaaaacac ggatcaaaat
     1081 gttttatcgc cccgcacact tgaaggggtt ggtcaacgat gcgtggaaat cactggaatt
     1141 taaacgtagt gaccattcac tcaatatata taacccaact gaatattacg tcgtatttgc
     1201 cggactggca gtcgataaag ccgatctcac aagcaaaatt gaatatatcg cgcccggaga
     1261 acataaacag ttaccacttc cagcatctgg cggaaagaac gtgaaatggg ctgcgatcaa
     1321 tgattatggc ggcagttccg ggacagaaac tcgtccactg caataacaaa tataaaaaca
     1381 caggtcatca gggaatgcca caacgacacc accagggaca taaacgcaca ccgaaacagt
     1441 tggcgctcat tatcaaacgc tgtttgccga tggtgctcac tggcagcggc atgctttgca
     1501 ctaccgctaa cgccgaagag tattatttcg accccattat gctggaaacc acaaaaagtg
     1561 gtatgcaaac aaccgatctg tcacgttttt caaaaaaata cgcacaacta ccaggaactt
     1621 atcaggttga tatctggctg aataaaaaga aggtttcaca gaaaaaaatt acatttaccg
     1681 ccaatgcaga gcaacttctg cagccacagt ttacggtaga acaactacgt gagctgggta
     1741 ttaaggtgga tgaaatcccg gcgctggctg aaaaagatga cgatagcgtg atcaactcgc
     1801 ttgaacaaat cattcccggt acagctgctg aatttgattt caatcatcag cgacttaatt
     1861 tgagcattcc ccaaattgca ctgtaccgtg atgcaagagg ttacgtctcc ccttctcgtt
     1921 gggacgatgg tataccaacg ctgtttacca actactcgtt tacaggttct gataaccgtt
     1981 accgccaggg caatcgtagc caacgacagt acctgaatat gcaaaatggt gctaattttg
     2041 gcccctggcg attacgtaac tattctacgt ggacacgcaa cgatcaggcg tcaagctgga
     2101 acactatcag tagttattta caacgtgata tcaaggcgtt gaagtctcag ttgcttctgg
     2161 gagaaagcgc caccagcggc agtatttttt ccagctacac ctttactggc gtgcaactcg
     2221 cttccgacga taatatgttg ccaaacagcc agcgcggatt tgccccaacg gtacgcggta
     2281 tcgcaaacag tagtgcaatc gtgactatca ggcaaaatgg ttatgtgatc tatcaaagca
     2341 acgtgccagc gggtgccttt gaaattaacg atctctaccc ctcttccaac agcggcgatt
     2401 tagaagtcac gattgaagaa agtgacggta cacaacgtcg ctttatccag ccttattctt
     2461 cattacccat gatgcagcga cctgggcatc taaaatatag cgcgaccgct ggacgctatc
     2521 gcgctgatgc aaacagtgat agcaaggaac ccgaatttgc tgaagccacg gcaatatatg
     2581 gtttgaataa tacttttacg ctgtatggcg gcctgctcgg ttctgaagat tattatgcgc
     2641 tggggatcgg tatcggcggc acacttggcg cactgggcgc gttgtcgatg gatatcaaca
     2701 gagctgacac ccaattcgat aaccagcact cttttcatgg ctatcaatgg cgtacgcagt
     2761 acatcaaaga tatcccggaa accaacacca atatcgctgt cagctactat agctatacca
     2821 acgatggcta ttttagtttt gatgaagcca atacccgcaa ttgggactat aacagtcgcc
     2881 aaaaaagtga aattcaattc aacatcagcc agacaatatt tgatggggta agtctgtatg
     2941 cctccggttc acagcaagac tattggggca ataacgagaa aaacaggaat atctctgttg
     3001 gggtttccgg ccagcaatgg ggaattggtt acagcctgaa ttatcaatac agccgctaca
     3061 ctgatcaaaa taatgaccgc gcactctctt tgaatctcag tattccgtta gaacgctggt
     3121 taccgcgtag ccgggtttcc tatcagatga ccagccagaa agatcgccca acccaacatg
     3181 aaatgcgtct tgatggctca ctgctggatg atggtcgcct gagctatagc ctggaacaaa
     3241 gtctggatga cgataacaac cataacagta gcctgaacgc cagttaccgt tcaccttatg
     3301 gaaccttcag tgccggatac agttacggta atgacagcag ccaatacaat tacggcgtta
     3361 ccggcggcgt ggttatccat cctcatggcg tgacgctctc gcaatatctg ggcaacgctt
     3421 ttgcgcttat cgatgctaac ggggcatctg gcgtgaggat acaaaactat ccggggattg
     3481 ctaccgatcc ctttggctat gcagtggttc cttatctcac aacttatcag gaaaaccgtc
     3541 tctcggtaga tactacgcag ctgcccgata acgtcgatct tgaacaaaca acacagtttg
     3601 tggtgcccaa cagaggtgca atggtagcgg cgcgtttcaa cgccaatatc ggttatcgcg
     3661 tacttgttac agtcagcgat cgcaacggta aaccgttgcc ctttggcgct cttgccagca
     3721 acgatgatac ggggcaacaa agtatcgtcg atgagggcgg catactatat ctctctggga
     3781 tatcgagtaa atcacaaagc tggactgtac gctggggaaa tcaggcagat caacaatgtc
     3841 agtttgcttt tagtacaccg gattcagaac caacaacctc tgtattacaa ggcacagcgc
     3901 agtgccatta aggataaaaa aatgaaaaga gcgcctctta taacaggact tttgttgata
     3961 tccacatcct gcgcttatgc ctcctcagga gggtgtggag ccgacagcac tagcggtgcg
     4021 acaaattaca gcagtgtggt tgatgatgtt acggtgaacc agacagataa cgtgacagga
     4081 cgggagttta cctctgcaac gctaagtagc actaactggc aatacgcctg ttcctgctct
     4141 gcgggtaagg cagttaaact tgtctatatg gtcagccccg tacttaccac cactggacat
     4201 cagacaggat attacaaact caatgacagc ctggatatta aaaccacatt acaggcaaac
     4261 gacattccag gactcacaac cgaccaggtt gtctctgtta acacccgatt cacacagata
     4321 aaaagcagca ccgtatattc tgctgcaacc caaacgggtg tttgccaggg tgacacgtct
     4381 cgttatggac ccgttaatat tggtgcaaat accaccttta ccctgtatgt caccaagcca
     4441 tttctcggct cgatgaccat tccgaaaacg gatattgccg tcattaaagg cgcgtgggtc
     4501 gatggaatgg gaagcccgtc tacaggtgac ttccatgatt tagtcaagtt atcgatacag
     4561 ggaaatctca ccgccccaca gtcgtgcaaa attaatcagg gcgatgttat taaggttaat
     4621 tttggattca tcaatggtca gaagtttacc acccgcaatg ccatgccaga cggttttact
     4681 ccagtagact ttgatatcac ttatgactgt ggtgatactt caaagattaa aaactcgttg
     4741 caaatgcgca tcgacggtac aactggggta gtagaccagt acaacctggt cgccagacga
     4801 agaagttcag acaatgcgcc cgatgtcggt attcgtattg aaaatctcgg tggcggagtt
     4861 gcaaatattc cttttcagaa cggtatcctt cccgttgatc cttccgggca tggcaccgtc
     4921 aatatgcgcg cctggccagt taatctggtc ggtggtgagc tggaaacagg aaaatttcag
     4981 ggcacagcca ccattaccgt catcgtgcgg taaaagaaga cgttgcgttt tgattaacgc
     5041 aacgcttcgg cactcttacc cctgctgctc cagcgcatac ttatatagcg catttttctt
     5101 cacgccgtga atttctgcgg ccagcgccgc cgcttttttc agcggcagtt ctgcctgtag
     5161 cagcgccagc gtgcgcaggg catcggcggg taagtcttct tcctgtgctt tatgaccttc
     5221 gacaatcagc accatttcgc ctttgcgacg gttttcatct tcctttaccc acgccagcag
     5281 ctcgccaacg ggcgcgccgt gaatggtttc ccaggttttg gtcagctcac gcgccagaac
     5341 cacgtagcgg gattcgccta ataccgcaac gatatcttcc aggctatcta acagacggtg
     5401 ggtagattca taaaaaatca gcgtgcgcgg ctccgcttca atggctttta gcgcatcacg
     5461 gcggcctttt gatttggcag gtaaaaagcc ttcgtaacag aaacggtcag agggtaaacc
     5521 cgcggcgctt aacgcagtga tagcagcaca cggtccgggt agcggcacca cacgaatccc
     5581 cgcttcacgg caggtacgca ccagatggta gccaggatcg ttaattagcg gcgttccggc
     5641 atcggaaacc agcgcaatgt tttgcccctc ttgcagcttc gccagcagcg tttcggcttt
     5701 ttgttgttcg ttatggtcgt gcagcgcaaa caaccgggca ttaatcccaa aatgttgcag
     5761 caataaaccg gtgtgacgag tatcctcggc ggcaatcaga tcaacggcct gtaatacctc
     5821 taacgcacgc tgggtgatat ccgccagatt gccgattggc gtcggtacaa tgtaaagctg
     5881 gccctgagaa ttatccgccg attggtgttg tttcattgtg tcgtccgtat tgccgattta
     5941 atattgagca ttgcgtaaaa aaaatatcac tggatacatt atggtaccct caacattttc
     6001 tcgtttgaaa gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgtcggttg
     6061 tggcacccat actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc
     6121 tgccttttat cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt
     6181 actcgccatt cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa
     6241 ccaactaccg caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga
     6301 gattaaactg gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaattacacc
     6361 cgccgattta gaacaaaacc agcaagcccg ttactggcag gcaaaaatcg atgccagcca
     6421 ggggcgtcct tccattgatt tactgcgcgc gttaattgct caggaaccgc tgcttggcgc
     6481 gaaagaaaaa cagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga
     6541 acaggcgaat acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct
     6601 gcagcgcgtc tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga
     6661 ctggcagaaa cgttatccga acaacccggg cgcgaaaatg ctgccaacgc agttggttaa
     6721 cgtaaaagcg tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cactgaatgg
     6781 ccaggcagcg gtatttggtc gcactattca gcaaggcttt gaagcggcga aaaatatcgg
     6841 cactcagcca gtggcagctc aggtagctgc cgcacctgcc gcagacgtag cagaacaacc
     6901 ccaaccgcaa accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac
     6961 cggtgaacag cctgcagccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc
     7021 ggtaagcgca cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc
     7081 acttagccag atcttaagcc aggttcagca ggatggcgcg agtattgtgg tcggtccgtt
     7141 gctgaaaaat aacgttgaag agttgctgaa gagcaacact ccgctgaacg tactggcact
     7201 gaaccagccg gagaatatcg aaaatcgcgt caatatttgt tacttcgcgc tttcaccgga
     7261 agacgaagcg cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt
     7321 gctgatccca cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca
     7381 gaaactgggc ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc
     7441 gggtgttaac ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac
     7501 aaccgactcc ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca
     7561 gttcaccaat aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat
     7621 cgcttttatc aaaccgatga tcgccatgcg taacggtagc cagagcggtg caacgctgta
     7681 cgccagctcc cgcagtgcgc aagggaccgc tggcccggat ttccgactgg agatggaagg
     7741 cttgcagtac agcgaaatcc cgatgctggc aggcggtaat ctaccgttaa tgcagcaggc
     7801 actcagcgcg gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc
     7861 ctggtcgctg gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg
     7921 taataccgga agcctgacgg ctaacccgga ttgcgtgatt aacaggaagt tatcatggct
     7981 acagtaccaa caaggtcagg tagtccccgc cagttaacca ccaaacagac cggcgatgcg
     8041 tgggaagtac aagcgcgtcg ctggctggaa ggcaaaggac tgcgatttgt cgccgctaac
     8101 gtgaacgagc gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt
     8161 gtcgaggtgc gttaccgccg ttctgcgctt tatggcggcg cggcagccag tgtgacccgc
     8221 agcaaacaac acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt
     8281 tttgatactg tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg
     8341 attaaggatg cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag
     8401 aattaaagct tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc
     8461 ggatgccatc tcccgtgcgg ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat
     8521 cctctgttgt ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat
     8581 caaccgtttc gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt
     8641 tgtcttaacg gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg
     8701 ggcgctgggt catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga
     8761 tattgtaaaa gcggtagaag ctgccgttac gcgtgatatg accattgtgg cattgaccgg
     8821 ctatgacggc ggtgaacttg caggtttgtt agggccacag gatgtggaga tccgcatccc
     8881 ttcgcatcgc agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga
     8941 tctgatcgat aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt
     9001 atcgccaatc gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt
     9061 agtgggtacc gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca
     9121 ggtggacgat ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat
     9181 taagaaagaa gcgcgcatta atgtaacggc ctatcagggc aaagtgctgc tggttgggca
     9241 gtcaccaaac gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc
     9301 caacgaagtg tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcgtctaa
     9361 tgatacgtgg atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc
     9421 gtccaacgtg aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga
     9481 acgtgaagcg aaagcggcgg cagatattgc cagccgggtg agcggcgtga agcgggtaac
     9541 tacggcgttt acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc
     9601 gtcttatcag gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc
     9661 gacatgtttt ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac
     9721 tgccaccatc gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat
     9781 cagtgacggc aaactcaccg ccgggagcgt catcaacaat gccagcgccg gagcagttcc
     9841 catacctgcc agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa
     9901 caagcatcct gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc
     9961 ggcatggggg aataaccaga cgcgagcggc acccaacacc agtactgcaa ggatgtaaac
    10021 cgggatcgta ctccagaaaa gcgtccatag cgccctgccc cagcggctaa aaaatccgcc
    10081 ctgtgcttcc ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca
    10141 tttttgcacc agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc
    10201 cgcaaaaccc cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg
    10261 attgcccatc cagaatgcca gcgcaccgcc catcgacacc tgttggcgac gcattcccgc
    10321 cgcgaccgga gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag
    10381 cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaac aaccagtcac gcgggatcag
    10441 cacctgaatc aacgaaccga ggatcacccc cagcactgcc gctttccata ccgcgaggaa
    10501 atagatcatc gcgtaatcca acgccgcttg ccacggatta gcatccgcct gcgcaaggat
    10561 agatttaccg atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca
    10621 tttcacatac cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg
    10681 aatgggcgtt gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt
    10741 atgataattg agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc
    10801 tcttttgcgt aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc
    10861 ctctgccagc atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggtaa
    10921 caaacggaac agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag
    10981 taacatcgac gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc
    11041 catctcccct ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact
    11101 gaccaccagc atatgctgcg cgcccagccg ccgcccggtt aatgcggtat ccactaccag
    11161 cgtgtaatcg gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag
    11221 acaacaaaac acaatgtcga tgggatcggt gacctgcgcc agcgcgtcag tcagttgcgg
    11281 atcatgggga ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat
    11341 tgcgttaact ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt
    11401 tgcgcctgta atcagtacct gactcatctt cgctccttta cagaattgtc taccttgcgc
    11461 tccacggctc atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgggaaa
    11521 ttcgcctcat ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat
    11581 cactgatgaa tttgaagatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg
    11641 acacgaagtg attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga
    11701 agccagcgtg accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct
    11761 gttgctaccg ggcggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt
    11821 tacccgtgat tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct
    11881 gctgatcagc gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat
    11941 tgatgttaaa aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca
    12001 gctggttacc agccggacgc cggacgatct gccagcgttt aaccgcgagg cgttacgcct
    12061 gctcggtgcc tgagccgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg
    12121 tgaatttgat ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg
    12181 gaaagcgacg attgtacgcc ttgcgcgcga ggtcgctttc ctcaccttcc agcctgcgga
    12241 tctcaccttt aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg
    12301 ttccggcaac cgcagcctgc ggcccactca tctgcgcatg acgcgttttt tcttccgtca
    12361 aaatgtagaa ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccagagtt
    12421 ccccttcctg ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg
    12481 tgatgagtgt ttccattgct gttctctttt atactgtggg cctgacctta acatgcgacg
    12541 cagacgatga caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc
    12601 gggatcacca cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa
    12661 gcactgcgcg ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg
    12721 ctggcgttac gggcggaata tcgcgttaaa caactgacca aacggcaaaa agagcgtctg
    12781 gtagcggaag gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa
    12841 agcgattgaa atgctcgtga tactcaacca gaccagttac gccattcaac gcgtcatccg
    12901 ctaaacgatg tacctggaag gcgctttcag tgcctggcca gcggcaacgc agatcgtgat
    12961 gtgccgccag ttcaaagcca aaacggctgt acagcgccgg atcgcccagc gtcaccactg
    13021 cggcatagcc gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc
    13081 cctgtccacg gtatttttca tctaccgcca acggtgccat gccgacccat tgcaggtctt
    13141 cgccctgcac atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg
    13201 tcgccaccag ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt
    13261 ccgcatcact ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa
    13321 tttctactcg aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt
    13381 cattcccgcc tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat
    13441 ttgctccagt tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc
    13501 ttcaatcacc agccgacgct ggaagaagag cgtatccgga tcctgtttac gcgccgcaat
    13561 catcagcaga tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac
    13621 caccagtttg ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat
    13681 acttaaccag cggccttcaa gaaactccag ctcgccatca tccagcgcct gacggaattg
    13741 ccagcttaag acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg
    13801 tacactcaac agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag
    13861 ttttactccc tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt
    13921 ataaatcaac aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa
    13981 atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt
    14041 gatagcgcaa ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc
    14101 gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac
    14161 caatgcccgt cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag
    14221 ttttgtccat caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga
    14281 cggttacgcc cgttggcagc gtgccgtgga tatggcggcg cagctgggtg ccgacgcgct
    14341 gatcctcgcc gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg
    14401 tcatgtgtca gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca
    14461 ttttgacgtt gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct
    14521 ggcacgggtc acacctgtcc cgctggaagt gtttgctttc ggcagcctgt gcattatgtc
    14581 ggaaggtcgt tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc
    14641 gtgttctccg gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct
    14701 gaacgaagtg ctgatcgatc gttatcagga cggcgaaaac gcaggttatc cgacgttgtg
    14761 taaagggcgt tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct
    14821 caataccctg gaactgctac cggagttaat ggcgtcgaat atcgcttcgg tgaaaattga
    14881 aggccgccag cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat
    14941 cgaccgttgt aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct
    15001 cgggtcgatg tccgaaggca cgcaaaccac ccttggcgcg tatcaccgta aatggcagtg
    15061 agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa agagacgctg
    15121 gaagattttt atcagcaggc cgccaccagc agcgccgacg tgatttatct tggcgaagcg
    15181 gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc
    15241 gccggaagtg gtaagcagat agtgctctcc acgctggcgc tggtgcaggc atcatctgaa
    15301 ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc
    15361 ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccggaca cgcgctgaac
    15421 tgctataacg cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg
    15481 tcggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagctgggc
    15541 attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc
    15601 gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc
    15661 attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc
    15721 aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct ggcatccatg
    15781 cagggcctgg ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc
    15841 gacgccttcc gcgctaatga aaatggcgct gcaccactgc cgttgacggc gaacagcgac
    15901 tgtaacggct actggcggcg gctggcagga ctggagctgc aagcctaagt aaatagctca
    15961 ctttgttaac aactttaact actctttaat gcagtattaa agattaatcg gtaacaaagt
    16021 gagctgttat gactgataaa accattgcgt tttcgctact cgatctggcc cccattcccg
    16081 aaggttcttc agcgcgagaa gcattctccc actctctcga tctcgcccgt ctggctgaaa
    16141 agcgcggcta tcatcgctac tggctggcag aacaccacaa tatgactggc attgccagtg
    16201 ctgccacgtc ggtattgatt ggctatctgg cggcgaatac caccacgctg catctggggt
    16261 ctggcggcgt gatgttgcct aaccactcac cgttggtcat tgcagaacag ttcggcacgc
    16321 tcaatacact ctatccgggg cgaatcgatt tggggctggg tcgtgctccg ggtagtgacc
    16381 agcgaaccat gatggcgcta cgtcgtcata tgagcggcga tattgataat ttcccccggg
    16441 atgtcgcgga gctggtggac tggtttgacg cccgcgatcc caatccgcat gtgcgcccgg
    16501 taccaggcta tggcgagaaa atccccgtgt ggttgttagg ctccagcctt tacagcgcgc
    16561 aactggcggc gcagcttggt ctgccgtttg cgtttgcctc acacttcgcg ccggatatgc
    16621 tgttccaggc gctgcatctt tatcgcagca acttcaaacc gtcagcacgg ctggaaaaac
    16681 catacgcgat ggtgtgcatc aatattatcg ccgccgacag caaccgcgac gctgaatttc
    16741 tgtttacctc aatgcagcaa gcctttgtga agctgcgccg tggcgaaacc gggcaactgc
    16801 cgccgccgat tcaaaatatg gatcagttct ggtcaccgtc tgagcagtat ggcgtgcagc
    16861 aggcgctgag tatgtcgttg gtaggtgata aagcgaaagt gcgtcatggc ttgcagtcga
    16921 tcctgcgcga aaccgacgcc gatgagatta tggtcaacgg gcagattttc gaccaccagg
    16981 cgcggctgca ttcgtttgag ctggcgatgg atgttaagga agagttgttg ggatagtgtg
    17041 tcttaacgcg ggaagcctta tccgagctgg caacgctgtc ctacatagac ctgataagcg
    17101 aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc tgattactga
    17161 tacaccggca gtaaattaaa gctcgataaa atatgcacca gtgcgttgcc gacgccaaac
    17221 accagaatca gcgcaatcat cggcttgcca ccccagacgc ggaatttcgg gctgccaaag
    17281 cgtttacgcg atgcgcgggc cagcagtgcc ggaacaattg ccgcccagat ggtagccgct
    17341 aaaccagcat aaccaatggc gtacaggaat ccgttcggga acagcaggcc ccccacgaca
    17401 ggcggggcaa aggtcagcaa tgctgttttc aagcggccca cagctgagtc atcgaaacca
    17461 aacagatctg ccagatagtc aaacaaaccc agcgttacgc caaggaacga actcgctacc
    17521 gcaaagtttg agaacacgac cagcagcaga tccagactac ggctgttcag tacgccgctt
    17581 aacgcctgta ccagcacatc aatattaccg cccttctctg caataccgat aaactccgga
    17641 cgcgggatgt tacccatcgt cgccagcaac cagatggtat acagcgccag cgccatcagc
    17701 gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata cttcatcagg
    17761 cttggcacgt taccgtgata accaaacgat gccagacaga acggcagggt catcaacaga
    17821 tacggtgcat aagacgcatt gctttcggcg acgttgaaca atgtcgcagg ctgcacatgc
    17881 cccagcaggc taccaaaggt gaggaagaag gtaatgactt tcgcccccag cacaatcgct
    17941 gtcatgcgac tgacggcttt agtgctcaac cacaccacaa acgctaccaa caatgcaaaa
    18001 ccaaaacccg ccgcccgtgc cgggacgttt agtgacatct ctgcgaaggt gtgatgcaga
    18061 atcgaaccac tggcagaaat ataggcatag gtcaggatat agagcacaaa ggcaatggaa
    18121 atgccgttga ccacgttcca gcctttgccc agcaaatctt tggtgatggt gtcaaaactc
    18181 gaaccgattc tgtaattcag gttagcttcc agaatcatca agccggaatg cagcatacag
    18241 aaccaggtaa agatcagcgc cgccattgac cagaaaaacc acgccccgga catgaccact
    18301 ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac gccgccaagc
    18361 agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct ccagtgaaaa
    18421 atagtgcgac tgcgttgtta tgtattgcac tgtaccagta cacgagtaca aaagacagaa
    18481 aaaaagcccc gatggttcaa atcggggctg tatatattat tttacagact gtgttcgctg
    18541 ttcagcgatg attacgcatc accaccaaaa cgacgacgac cggtagagtc atcacgacgc
    18601 ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac catcaccacg accaccttca
    18661 cggcgttcac cgctgaagtt acggccgcct tcacgacgct cgccactgaa accacgacca
    18721 ccgccacgac gctcaccgcc agtatgcggc tgtgcatcgc ccagtaattg catattcatc
    18781 ggcttgttca gaatgcgagt acgtgtaaag tgttgcagca cttcacccgg catacctttc
    18841 ggcagttcga tggtggagtg agaagcaaac agcttaatgt taccgatgta acggctgctg
    18901 atgtcgcctt cgttagcaat cgcaccaacg atatgacgaa cttcaacacc atcatcgcgg
    18961 cccacttcaa tacgatacaa ctgcatgtca ccaacatcac gacgttcacg acgcggacga
    19021 tcttcacggt cgccacgcgg gccacggtcg ttacgatcgc gcggaccacg gtcatcacgg
    19081 tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag agtacgttca
    19141 ccctgtgcca ttttcagcag tgcggcggcc agagtttcga gatccagctc ttcaccttct
    19201 gcagtcggct gaattttgct cagcagtgcg cggtattgat ccagatcgct gctttccagc
    19261 tgctgctgta ctttagcggc gaatttttcc agacggcgtt tgcccagcag ttctgcgttc
    19321 ggcagttcta cttccggaat agtcagcttc atagtacgtt caatgttgcg cagcagacga
    19381 cgctcgcggt tctcaacgaa cagcagcgcg cggccagcac gacccgcacg accggtacga
    19441 ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac caccaggctg
    19501 atacgctcaa cgtccaggcc acgggctgca acgtcggtcg caatcaggat gtccagacga
    19561 ccatctttca ggcgttccag tgtctgctca cgcagcgcct ggttcatgtc accgttcagc
    19621 gcggcgctgt tgtagccgtt acgctcaaga gcttcagcca cttccagagt cgcatttttg
    19681 gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg taccagtgct
    19741 tcgtttttac gcatacccca gacagtccag tagctctggc tgatgtcagg acgggtagtc
    19801 acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt aatgcgacga
    19861 atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg gatctgcgcc
    19921 ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc ttcatccaga
    19981 accagaccgc tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc cagcagacgg
    20041 cccggagtac cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg cacgtcataa
    20101 cgctggccgc cgtacagggc aaccacattt acgccgcgca tgtgtttaga gaaatccgtc
    20161 attgcttcag caacctgtac cgccagttcg cgggtcggtg ccagcaccag aatctgtggt
    20221 gctttcagct caggatcaag attctgcagc agaggtaaag agaatgctgc agtttttccg
    20281 ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg aatacactcc
    20341 gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag gataggagcc
    20401 ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt gtgcctcaaa
    20461 attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt tcattgaaaa
    20521 gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc cgttaaggtg
    20581 atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg tccgattctg
    20641 ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat cggtgctcaa
    20701 caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg ctgtccaaat
    20761 cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga tgctcagcga
    20821 gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt tcgctaatgt
    20881 tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat ttttcgaagt
    20941 gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg agataaagcc
    21001 acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga tcatcttgcg
    21061 ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc gcgtagttgt
    21121 aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca aaattgcctg
    21181 cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt cggattgcca
    21241 gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca tacaacactc
    21301 cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg gcaaggattt
    21361 gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt accgcgagga
    21421 cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct gtcgcaacga
    21481 aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg aatgaacgtc
    21541 ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc gcagcggagg
    21601 gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct gcagcaggtt
    21661 gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga tcaacttcca
    21721 gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc tcaacgcgtt
    21781 tgtcagcgat ttgagagatg tgtaccagac cttctttacc gccgccgatg gcaacaaatg
    21841 cgccgaagtc aacgatacgg gtcactttac cagtgtagac gcggcccact tcgatttctg
    21901 cagtgatctc ttcgatacga cgaatagcgt gtttcgcttt ctcgccgtcg gtcgctgcga
    21961 tcttcacagt accgtcatct tcgatttcga tggtggtgcc ggtttcttcg gtcagggcac
    22021 ggataacaga gccgccttta ccgataacat ctttgatctt gtccgggttg atcttgatgg
    22081 tatggatacg cggtgcgaac tcagagatat cgccacgcgg cgcgttgatc gcctgttcca
    22141 ttacacccag gatgtgcaga cgcgcacctt tagcctggtt cagtgcaacc tgcatgatct
    22201 ctttggtgat accttcaatt ttgatatcca tctgcagtgc agagataccg tcgcgggaac
    22261 ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg tcagacagta
    22321 caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg gcagctttga
    22381 ttggcacacc tgcgtccatc agcgccagag acgcaccgca cacggaagcc atagaagaag
    22441 aaccgttgga ttcagtgatt tcagacacaa cacgtacggt gtacgggaat ttgtccatat
    22501 ccggcattac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt tcacgacgct
    22561 tcggagaacc gaccatgccg gtttcgccta cggagtacgg agggaagttg tagtggaaca
    22621 ggaaggtatc ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca cgagcagtac
    22681 ccagcgttgc ggtaaccagc gcctgcgttt caccacgggt gaacagcgca gaaccgtgag
    22741 tacgcggcag cacgccagta cgcacatcca gaccacggat catatctttt tcacgaccat
    22801 cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac gtttttctcg atcgcgtgca
    22861 gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc gtcgcgatgg
    22921 tttcagattt gatgacatca acctgcgcat aacgctcttg tttgtcggtg atgcggtaag
    22981 catcgctcag acgagcttca gccagtgcag caacgcgcgc gttcagcgct tcgtttaccg
    23041 gctccggctg ccagtcccaa cgcggtttac cggcttcttt caccagttca ttgatgttct
    23101 gaataacaac ctgctgttgt tcatgaccga acactactgc gcccagcatc tggtcttcgc
    23161 tcagcagttc agcttcagat tcaaccatca gtacagcggc ttcagtaccc gcaacaacca
    23221 gatccagttt gctctctttc agctcgtcct gagtcgggtt cagtacgtac tggtcattga
    23281 tgtaacctac gcgggcagca ccaatcgggc cattgaacgg aataccagac agagacagtg
    23341 ctgcggaagc accaatcatc gcgacgatat ccgggttaac ctgcgggtta acagaaacca
    23401 cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg cgaatcgggc
    23461 gatcaatcag acgcgcgatc agggtttcgc cttcgcttgg acgaccttca cgacggaaga
    23521 agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg gtcagtggga
    23581 agaagtcctg acctggtttg gcttttttct ggccaacaac ggtaacaaat accgcggtgt
    23641 catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg gtttccagag
    23701 tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat gtaatatcct
    23761 ttctctttct tagacagtac cttacggcac tggtgttaat acccgatctt ctgcgcatcc
    23821 tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca ttagccgcgc
    23881 gaacctctgc aacggaagat cattcatagc aacaatacat tagtttccag tgaattgctg
    23941 ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc aagaattagc
    24001 gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta cgtttcaggt
    24061 agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg ctgtggtgat
    24121 cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc agcagtgcta
    24181 cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa acgattttag
    24241 ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat gaaaggacgc
    24301 cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa tttacgcgac
    24361 gttaagcggc agtattctac tcgtagcgac ctgttatcgc aagacggtta acattacgcc
    24421 gggtattcaa ccaccagacg acgaggcgca acgcggcctt catcgtcaat ttcgcccata
    24481 ccgataaatt tgccgttctc accttccgtg acgcgaacca gtccttccag cggcgcgcca
    24541 gatgtacgaa ccgggttacc atttttgaag taaacagaag acgttaacgg aagattcacc
    24601 accggatagt ccgaagctgg actgtccatt ggcatcagta atggatcaag taactccgca
    24661 gctggaatat cctgctgttc ggcttgttcg accagttcac gcagatgttc cagggtcacc
    24721 atccgttcaa ccggatattt acttaccgcc agacggcgca ggtaaataac atgcgcgcca
    24781 caaccgagtt tctcacccag gtcgtcaatg atagtgcgga tataagtgcc ttttgagcag
    24841 tgaatttcca gctccagctc attgccttca tggcgaataa acagcaattc ataaacggta
    24901 atcggacgcg cttcacgcgg aacttcaatg ccctgacgcg catattcgta cagttttttg
    24961 ccctggtatt tcagtgctga atacatcgat gggatctgtt cgatatcgcc acggaaagta
    25021 tccagtgccg ccgccagttg ttctgcacta aaggttaccg gacgctcttc aacgatttgt
    25081 ccgtcggcgt cagaagtatc agtacgctgt ccaagacgcg caatgacccg atagcgtttg
    25141 tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat cggcaacatg
    25201 ccggtcgcca gcgggtccag cgcaccggta tgaccggcac ggttggcgtt ataaatccgt
    25261 ttcacttttt gcagcgcatc gttgctggac ataccctgag gtttatccag caacaaaacg
    25321 ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg ctgtcgtccg
    25381 ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt gacatgcgca
    25441 tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata cgcaggcgca
    25501 tcgctttccc cagcaggctg cggatgaaac cagaagcttc ctgcaacgct ttgatgcccg
    25561 ctttaactgc gtcttcatct ttgtcgttga ggaaagtgac atacactttg gcatacgcca
    25621 ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccagacga ggatctttaa
    25681 tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg cgctgcgggc
    25741 gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt agcccctttt
    25801 taaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg cgctagcgtc
    25861 gcaacaggca atccatgccg gatgcggcgt aaacgcctta tcccgcatag aaccctaaaa
    25921 accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca catcgccagt
    25981 gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac ggacttcgtt
    26041 aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcatagataa ccacgttgtc
    26101 acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca tacagcctgc
    26161 gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac cgataatctg
    26221 ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt cgatcaggtt
    26281 atagatgacg gagtagtaac gtagatccag gctttcggat tcaatcactt tacgtgcaga
    26341 ggcatcagca cgtacgttga agccaaccag gatggcgttg gatgccgcag ccagggtggc
    26401 gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa cttcgtcagt
    26461 agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt ctgccttcag
    26521 gacgatattc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt tctcgagttt
    26581 agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat agagtgcaac
    26641 ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag ccggaacacc
    26701 ggacaagccg aggatttcca ccggaatgga cggacccgct tccagcactt cctgacccag
    26761 ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga tatcgccctt
    26821 gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt tatcgaggaa
    26881 ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct ccagaacttc
    26941 cgcctgcagc aggatagcgt ccagcagttc atcgataccg gtacccgctt tcgcagatac
    27001 gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccgtact gggagagttc
    27061 gttcttaacg cgatccggat cggcttctgg tttatcgatc ttgttcactg caaccaccac
    27121 cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca ttacgccgtc
    27181 gtcggcagca acaaccagga ctacgatgtc agttgcctgc gcaccacgtg cacgcattga
    27241 agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt cagtttcaac
    27301 gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcca ctttcgttga
    27361 acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga tggtcacaac
    27421 cggagcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg cctcttccag
    27481 ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca gctgtgcggt
    27541 ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca tcgctttgat
    27601 gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga tagtttcgcc
    27661 gatcacaacg tcacggttaa cggcctgagc aggcttctgg aagccttgct gcagcgaaga
    27721 acctttacgt tttccgccct taccgccacg tactgctgcg cgtgcttctt cacgatcagc
    27781 ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt tacgaccacg
    27841 gccacggccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag catgttgaga
    27901 agtagtgacg tgataatcgc tggaatcctc agtcggttcc gcgttatcag tccatttgtt
    27961 ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt cgagtttacg
    28021 acgcgcttct tcttcggctt tacgcttgag ctctgcagct tcctgctcac ggcgggcttt
    28081 ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt ctttttccgc
    28141 agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg cctcacgttc
    28201 agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg cttgctcttc
    28261 cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt
    28321 cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc caccggtacc
    28381 aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt
    28441 tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag agtcgtcagc
    28501 agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg
    28561 ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg ctacagttta
    28621 ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg ctccggcttt
    28681 ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg cgagatcttc
    28741 cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta ccccttcaag
    28801 gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg cctgtgcaat
    28861 ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct catcaaggcc
    28921 ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg tcgagaagcc
    28981 ttcctctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc
    29041 gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg tcatcacgtt
    29101 gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac ggccaatcgc
    29161 ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt catccaccac
    29221 gatagaagca acgtctgctg gtgccattgc gttaatcaca aactgcgccg ggttatcatc
    29281 ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct gaacacgcgc
    29341 gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc
    29401 gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc
    29461 gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg aacgagtgac
    29521 gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac gaacgcggtc
    29581 gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg cttcagcgtt
    29641 gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat
    29701 ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt cacgcacttt
    29761 ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa cagactcaat
    29821 ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg cggcttcaag
    29881 ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac ggaaggtgtc
    29941 aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt catatttttt
    30001 ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc
    30061 cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg cgggcttttc
    30121 acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat tactcagcgc
    30181 gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat
    30241 aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag tcacctcttc
    30301 tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac ccggtgagga
    30361 gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag cacttacctg
    30421 gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa tatagatgcg
    30481 cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac ccagggcctc
    30541 aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc ccacccccaa
    30601 gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa aaaccccgat
    30661 aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga gcactttcca
    30721 gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat ttgaaaaagg
    30781 actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg ggttatgagc
    30841 ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc tactcgtttt
    30901 gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt aaaatctgca
    30961 ggcattatag tgatccacgc cacattttgt caacgtttat tgctaatcac gtgaatgaat
    31021 atccagttca ctttcatttg ttgaatactt ttgccttctc ctgatccccc ttaagcgcat
    31081 tattttacaa aaaacacact aaactcttcc tgtctctgat aaaagatgat taaatgaaaa
    31141 ctcatttatt ttgcataaaa attcaatgag agcggaaatc caggctcatc atcagttaat
    31201 taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt
    31261 ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat gcgacaaaag
    31321 ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga ggattatgat
    31381 gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat cgactgccgc
    31441 aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca taacaccacc
    31501 ggtggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg caccatgctg
    31561 gttgctgcta tgaaagaaga tggcgtgaat atctggggtg acggcagcac ctataaagga
    31621 aacgatatcg aacgtttcta tcgttacggt ctgctgacca atgctgaact gcagatttac
    31681 aaaccgtggc ttgatactga ctttattgat gaactgggcg gtcgtcatga aatgtctgaa
    31741 tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagctta ctccacggac
    31801 tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc
    31861 aaaatcgtca acccgattat gggcgtgaag ttctgggatg agagcgtgaa gatcccggca
    31921 gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg taaaaccttt
    31981 agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca cggtctgggc
    32041 atgagcgacc agattgaaaa ccgtatcatc gaagcgaaaa gccgtggtat ttacgaggct
    32101 ccggggatgg cgctgctgca cattgcgtat gaacgtctgc ttaccggtat tcacaacgaa
    32161 gacaccattg aacagtatca tgcacatggt cgtcagttgg gccgtctgct gtaccaggga
    32221 cgttggtttg attcccaggc gctgatgctg cgtgactctc tgcaacgctg ggttgccagc
    32281 cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc aatcctgaat
    32341 accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa aggcgactcg
    32401 gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaacctgga tatcaccgat
    32461 acccgcgaga aacttttcgg ttatgccaaa accggcctgc tttcctcctc tgccgcttca
    32521 ggcgtgccgc aggtggataa tctggaaaac aaaggccagt aattcgacat caaccctgcc
    32581 cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc atcacataat
    32641 cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt gcaatcactt
    32701 cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta ataaggtaat
    32761 tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacacca tccccaagaa
    32821 cggggctata ccagataaac atcggaacat gatatgcctg ctggctggcc tccctgcctc
    32881 catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg gcaaaataca
    32941 tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa ctatctgtgt
    33001 aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac acggcgctgg
    33061 attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga acaatcagtt
    33121 tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac aattcatcaa
    33181 atcctctgac ataaactgtt tccatggcac gcatggcgat actggtaact gctgtaccat
    33241 tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct tgattagcca
    33301 tattaataat gttgtcgggg taattatgaa tgtcatggct caaaacagaa tcagcagtta
    33361 aagaaagggg aaccgacagc gcagtgtaag gtgcaccgct tattgcttga ttaaacagtt
    33421 tgatctgttt tctttgtgct tcgacttgcg gtgtcgtaga gcgtgtatat ccatacaaag
    33481 acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca atacctgtat
    33541 ccctgactga taattgaaaa tacggcactg tgtttgcaat tgagagtaat ctttgatgct
    33601 ctttcgctgc taaagcaaaa taattgagat tgaaaaacgg cgtataggta gcaaatcgcg
    33661 acgctagtat atatggactg aacgcatttt tatttttggc atctttataa gcaaattgac
    33721 aagcggaaaa taaacttccc gaaatgacaa tcagcaataa tattcctgtc actttttttg
    33781 tcggcaagga aacatcatat tttattatta cagccaaaaa aagaagggat aaaaaggcaa
    33841 aagcacatag ataagggcta tacattccca gcattttggc aacttcatcc ggatcactct
    33901 gcagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac catgaataga
    33961 ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca agcaggcgaa
    34021 gcggaaggga tgatatacgt ttaacaacca ggatggtcag aacgaagaac agcaatcgct
    34081 gaaccatatg cgctccggaa gaggcgacta agttcgtaat accaaaaaca ataacactaa
    34141 gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact gtcatccaga
    34201 aatccaagtt cacaaccaat ataaacaatt atcaacaatg tgcaatttta tatcagacat
    34261 gtttttcaac tctgatccat atcagaacga aacactgtta gttttttctt tttaaatgat
    34321 gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa acattacgca
    34381 gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag aagtaaacat
    34441 atccgtattc gtaccggaac tggagttaac acaataaagt gccaattatg tcagcagtag
    34501 ggaaaagttt ataacgaagg cgtaccggca ccatcgtttc aaggtgccag ctacgagtaa
    34561 agcaactgga cgagatacag atacctgaca accattcctc agaccaggac caaaacgaaa
    34621 aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga cttgaacccg
    34681 taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca ccacctcggc
    34741 acggaaacta ctttttagtt cgggatatcg ctggtcggct tagccggagc agctggctga
    34801 gtttgttcgg ttttcgccgg cgcactcagg ttttcccatt cgctaccttt attggttttg
    34861 ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc cagcaacgcc
    34921 gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc ggaagcgcct
    34981 gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat cagaccaaca
    35041 aggccaattg ccacaataag gaaaactacc aaaagagctt cgtacataat caacctgttc
    35101 cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt taatctttcc
    35161 cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc aattttatcg
    35221 cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga
    35281 caatcgccac ctttttagcc agttatctaa cgctttaaac ggcttttact gcatcggcga
    35341 tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta
    35401 acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc gccgcttcaa
    35461 cctctgcggt cacagctttg actgattcat gctccagcgg atcgccgcta cctgcggtgt
    35521 aacgcacgtt taccaggatt tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc
    35581 tcatatggtt acgcgccatc gccgccagca cctgcaaacc agcaacgatg ccgtcaccgg
    35641 tagtggtttt atccagcagg atcacatgac cggaattctc tgcaccgata cgccagcctt
    35701 tctcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca aatggaattc
    35761 ctaactgttt caacgccagt tcaagcccca tgttgctcat caatgtaccc acagcgccac
    35821 cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc tgatcgccat
    35881 cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa
    35941 taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg tcggtagccc
    36001 ccacttcggc attgatgttt acaccgttcg gctcacaacc gatagcgata acgttcgccc
    36061 ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca caatccacca
    36121 caatcttcag ttcactaagg ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat
    36181 agcgacccgc agcatcaacg atacggctgg ctttacccag ttctgccgaa tcaacgcagc
    36241 tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc agtttggtgc
    36301 cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgcgat gcagatatca
    36361 caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt gttggcatcg
    36421 ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt gctgactcca
    36481 gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag ccgtggcgcg
    36541 ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggggtg atcggcgcat
    36601 cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc atagcgtttg
    36661 ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt ttctttgacg
    36721 tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca ggccagacta
    36781 ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga ttttcgtgac
    36841 atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa
    36901 tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa tttctctttt
    36961 gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt cacttctgca
    37021 aagacatcgt catacttcgg agcttcctgc atggttttgg gatttccctg catatgcatc
    37081 agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc ggaaagggag
    37141 cggatatcat taataatgtg agcgccaact tttgctgact cacggatgac ttctggtttg
    37201 gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac cacaggaata
    37261 acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt ggactcgcca
    37321 ccaacgtcaa tgatcgtcgc gccagcgttg atcatcagat tcgcatgttt caccgcatct
    37381 atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt gaggatcccc
    37441 attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt catgatgtta
    37501 tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa
    37561 tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg gtgttacccg
    37621 ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca ttgtcgccag
    37681 aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca
    37741 tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca tctttcatcg
    37801 catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag ttacgttcaa
    37861 tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata tgtttcgctt
    37921 tcgctacgct acggccgagg aacacttcac cttcttcttc cgcgtacagc agtggaccca
    37981 atttctcaga gaagccccac tgagtcacca tgttacgtgc caggttggtc gcaactttaa
    38041 tatcgttgga cgcaccggta gaaacatgtt ccggcccgta gatgatctct tctgccagac
    38101 ggccgccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg ctgattgcgt
    38161 cgccctcagg caggaagaaa gtcacaccca acgcacgacc gcgtgggata atcgtcactt
    38221 tgtgcaccgg atcgtgctcc ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt
    38281 aagccgtcga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc gcacccatca
    38341 tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag
    38401 cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag aaaccaggag
    38461 taccacgggc aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa
    38521 ccttcaggat ctgctcacga ccgcgaacat ctggcaagcc aaccacaacc tgacggtcga
    38581 aacggccagg acgcagcagc gctgggtcga gaacgtccgg acggttagtc gcggcgataa
    38641 cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg ttcagagtct
    38701 gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct acggcgtcga
    38761 tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa
    38821 cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag atagtaaaga
    38881 acggaacttt cgcttcgcct gcaatcgctt tcgccagcag cgttttaccg gtacccggag
    38941 gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag cggctcggct
    39001 cgcgcagata ctcaaccagt tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt
    39061 cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg
    39121 acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc cagacaccaa
    39181 tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt ggttcttcag
    39241 gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtagt ttcggatcct
    39301 gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg atttcacgtc
    39361 cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag gtagagtaat
    39421 ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac atcagcacaa
    39481 cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga ttaacctctt
    39541 attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt tcaaactttc
    39601 gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact tcccgcgaac
    39661 gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag cgaatttccc
    39721 ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg ccacctggcg
    39781 ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca cgggggatat
    39841 ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc tggactttgc
    39901 tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa tcgccctgaa
    39961 gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg atgcggcctt
    40021 tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca ccaaggtcga
    40081 caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca agtttaaacc
    40141 aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg ctaaagtgtt
    40201 cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa ctttcccatg
    40261 gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt gatatatggg
    40321 agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac gatgaatctg
    40381 agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc agttgttctg
    40441 cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc gttagagcac
    40501 catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac cttgatcgtg
    40561 gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg
    40621 ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta tcctaaagtt
    40681 acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa aatgccacgc
    40741 tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa tacatcaaca
    40801 tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa ttctacttcg
    40861 ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag gccacgagca
    40921 ataggtgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc gccaacgatg
    40981 cgataagtct gttcttcgtc agaatcgaga ttcagcaccg ttacggtagc accaaaaata
    41041 acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga cagcttggct
    41101 tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc gtggtattcg
    41161 gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat gatttcagga
    41221 cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc
    41281 atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca agtcttcacc
    41341 caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag caaaaaaata
    41401 ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc attttaccct
    41461 gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta tgacggctcg
    41521 attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg attgaccagc
    41581 tgtatagcgt tcagtgttca ggccgcaaat gttgatgagt acattactca actccccgct
    41641 ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc tattgattac
    41701 cacagtcagc agatggcgct gcccgccagt acccagaaag tgattactgc gctggcggcg
    41761 ttgattcaac tcggccccga ttttcgtttt accacgacgc ttgaaaccaa aggcaatgtg
    41821 gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa
    41881 cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat
    41941 ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc cggctggcca
    42001 tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ccgccatagt tgaccgcaac
    42061 tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt tatacgcgtg
    42121 gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg tggttctgcc
    42181 gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt tacgctgacg
    42241 ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca ggatggagcc
    42301 agctatgccg gtgcaattct gaaagatgag ttaaaacagg cgggtatcac ctggagcgga
    42361 acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag taaacagtcg
    42421 gccccgctgc acgatctgct taagattatg ctgaaaaagt cggacaacat gatcgccgat
    42481 acggttttcc gcatgatagg ccatacgcgc ttcaatgtgc ctggaacatg gcgggccggg
    42541 tcggacgctg tgcgtcagat cctgcgccag caagccggtg tcgatattgg aaacaccatt
    42601 attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac catgatgcag
    42661 gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat gctgccgctg
    42721 gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg cgtggatgga
    42781 aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg attcattacc
    42841 acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc agtagaaccg
    42901 gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt gtataaagat
    42961 atttatcaga acaattagtc aaaaagaaac cccggcacat ggccggggtt ttgctgatta
    43021 acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcgt catcccagtc
    43081 ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga tgataatcat cccacatgaa
    43141 ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt caatgataaa
    43201 ggtcatcaca tcccagcaga gatctttcac gcccagtcca ctcgccgcag agatcagata
    43261 atatttatct tcccagccca gcgcttcagc gatcgctttc gctttctctt cggcttctgc
    43321 cttatccagc agatcgatct tattgaagac taaccaacgc ggtttcgccg ccagatcctg
    43381 gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat cggtgccgtc
    43441 aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt gcttcaggaa
    43501 gcgaatgccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa tatcggcaac
    43561 aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca gagtggtaaa
    43621 cggataatcc gccactttcg gtttagccgc cgataccgca cgaataaagg tcgatttacc
    43681 cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca gcagcagctc
    43741 acgcttatcg cccggcgtac cgttagtttt ctgccgtggt gtacggttaa ctgacgattt
    43801 gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac gctgaccgtg
    43861 tttggtcata tcgcccatgg tttcaccagt accctggtcg attacacgcg tacctaccgg
    43921 cactttaatt gtgacgtctt taccgcgctt accggtacag tcgcggcttg cgccattctg
    43981 accgcgctct gcacggaaag atttttcaaa acgataatcg ataagcgtgt tcaggttctc
    44041 gtcggcttcc atccatacgt caccaccatc accgccgtcg ccgccatccg ggccgccttt
    44101 cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac ctgcaacgac
    44161 cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt cgcctgcgcg
    44221 gggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat gaccaatggc
    44281 ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa ggtttaacat
    44341 cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa acagcgtaaa
    44401 cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag ccatcgcttc
    44461 cgccagggcg ccatatccta ccaaggtatt cagtccgcaa aaaattaaac atgcgagctg
    44521 ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg caattgtaca
    44581 taaagtgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata aaaccttttg
    44641 cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa ctccccaggt
    44701 gtaatcggtt agcttggtaa atatctcgac cagactggtg ttaaaaaaca tcaccaggcc
    44761 gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt tcaggataaa
    44821 tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg aagcggtcgg
    44881 actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc cggcggtcgc
    44941 cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg gcaacctctt
    45001 cttcaccgca agaatggcac ccaggccaat actcgccatc aagaaacggt aaaacacgat
    45061 tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg ccccccagca
    45121 aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca taccgtattc
    45181 cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc atttttaccg gttatcgaat
    45241 gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc gaaaaactta
    45301 ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga atttcacttt
    45361 accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag cgccagcgtg
    45421 gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt cgccaccgaa
    45481 acgcttaacg cccaggcgtt tagcttctga atcgcgaccg ttacgtgtgg agccgccagc
    45541 ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc agtaattttc
    45601 acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg acgacgaaac
    45661 ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt gattacgccg
    45721 ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat cagcacttca
    45781 gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac ggtctgacct
    45841 tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata aaactccgct
    45901 tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat agggcgcgaa
    45961 tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag aaaaaaaaca
    46021 caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcaccc tatattccta
    46081 accataaacc ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa agcccggctt
    46141 ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg ggtgttaatg
    46201 cggcaatcct tgagcagctt aattccgacg tccaactgat caatcagtta ggctattaca
    46261 tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc tgtactggct gcacgagctg
    46321 ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt atccacacgg
    46381 cgactctgct acacgacgac gttgtggatg aatcagatat gcgcaggggt aaagctaccg
    46441 ccaacgccgc atttggcaat gccgccagcg tgctggtagg cgattttatt tatacccgcg
    46501 cgttccagat gatgaccagc ctcggttcgc tcaaagtgct ggaagtcatg tcagaagccg
    46561 taaacgtcat cgcagagggt gaagttctgc aactgatgaa cgttaacgat ccggacatca
    46621 ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt gaggctgccg
    46681 cgcagtgttc cggaattctg gcgggctgta cgccgaagga agagaaaggc ctgcaggatt
    46741 atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat tacaatgccg
    46801 atggcgaaca gttaggtaaa aacgtcggcg acgatctgaa cgaaggtaaa ccgacgctgc
    46861 cgctgctgca tgcgatgcat catggcacac cagaacaggc acagatgatc cgtaccgcca
    46921 tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg aacgcttgtg
    46981 gatctcttga atggacgcgt cagcgcgccg aggaagaagc agacaaagcc atcgcagcgt
    47041 tacaggtgct cccggacacc ccttggcgag aagcactcat cggcctcgcg cacatcgctg
    47101 ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag tgcgcgggat gattccagta
    47161 aactccataa gaatacttat tcagcgtaat aatgcctgaa aaaccagtgt atacatcttt
    47221 atatattctg aatattcaca ctctttacag gaacttttta gagcaatcgg ccatcaggag
    47281 tatagtgatg ctcgacagaa gaagtgttct gaatgaaagc gactaactta aggagtgagg
    47341 aaagatggaa agtaatttca ttgactggca tcccgctgac atcattgcgg gtttgcgcaa
    47401 gaagggaacg tcaatggcgg cggaatctcg cagaaatggt ttgagttcct caacgctggc
    47461 gaacgcatta tcgcgcccat ggccgaaagg agagatgatt attgcgaaag ccctgggaac
    47521 tgacccctgg gttatatggc catcacgcta ccatgatccg cagacccatg agtttatcga
    47581 cagaacgcag ttgatgcgta gctacactaa accgaaaaaa tgagtggtct ggcggtagcc
    47641 ccgcgaacgg ggctgccagc tctcagacga ttactcgcct ttcacacgct caatatttgc
    47701 acctaaagcg cgcagtttgt cttcaatgcg ttcgtagcca cgatcgatgt gataaatacg
    47761 atcaaccacc gtcgtccctt ccgcaataca gccagccagc accaggcttg ctgatgcacg
    47821 cagatcggtt gccataacct gtgcgccaga aagtttttca acaccgtgac aaataacggt
    47881 attgctttcg atttcggcgt gcgcgcccat acggctcagc tctggcacat gcataaagcg
    47941 gttttcaaaa accgtttcgg tgatgaatcc ggtcccttct gccaccaggt tcaacagcgt
    48001 gaactgggcc tgcatatcgg tcgggaatgc cggatgcggc gcagtacgta cgttaacagc
    48061 cttcggacgt ttgccatgca tatccaggct aatccagtct tcgccgactt cgatgtccgc
    48121 tccagcatca cgcagtttcg ccagcacggc gtcgagagta tctggctgcg cgttacggca
    48181 gataattttg ccgcgagaga tcgccgccgc caccaggaaa gtaccggttt cgatacgatc
    48241 cggcagcacg cgatagacac cgccgcctaa acgttccaca ccttcgatga cgatacgatc
    48301 ggtgccctga ccgctaattt tcgcacccag cgtaatcagg aagttcgcgg tatcgacgat
    48361 ttccggttca cgcgctgcat tttcaataat cgtggtgcct tccgccaggg ttgcagcaca
    48421 catgatggtc accgttgcgc caacgctgac tttatccatc acgatatgtg cgcctttcag
    48481 acgaccatcg acggaagctt taacgtaacc ttcttccagt ttgatggtcg cgcctaattg
    48541 ttcgaggcca gaaatgtgta gatcaaccgg acgcgcaccg atcgtacaac cgccaggtag
    48601 tgaaacttgc ccctgaccaa agcgcgctac cagcggcccc agtgcccaga tagaagcacg
    48661 catggtttta accagatcgt aaggtgcgca gaatacatta acgtcgcggg catcaatatg
    48721 cacagaacca ttacgttcta ctttcgcacc cagctggctt agcagcttca ttgatgtatc
    48781 gacgtctttc agtttcggga cgttctggat ctctaccggt tcttccgcca gtagagcggc
    48841 aaaaaggata ggcagagcag catttttagc gccggaaatt gtgacttcgc cctggagctt
    48901 cgttggcccc tgaacacgaa atttatccat ttagtttgtt ctcagttaac aattcatatc
    48961 cgctaccggc gaatcgccca tagctcaaaa gccgttcagt ttgcgatcgc gcgcccactc
    49021 cgcaggggta tacgctttga tcgacacagc atgaatgcgg ttatccgcaa tatattccat
    49081 cagcggacca tagaccgtct gctgtttttt aacccgactc atgccgtcaa acaactcacc
    49141 cacggcaata acctgaaagt ggctgccatc gccggaaacg tggacttcct ggagggagag
    49201 agcgttcatc aacacgctct gaatttcatt attttccatg ggatcttcaa tcatcagtta
    49261 ataaaccagc gaaacatctt agagcaaagt tgcgctggca taaataagca aaaagcctcg
    49321 ctgataaatc agacaaggct cgacttgcag gcaggtttgc cggacaggcg gttaacgcca
    49381 tatccggcct gaaaaaattt aacggggcag aacatcagca ggcaaattat acaatttcgc
    49441 cagggtatac actttgtcgt ttaccccctg aagcgtcaca ttgttgccct gctttttcgc
    49501 cagatcgata agatggagca gcagtgccag tccacccgta tccacgcggg acacacggct
    49561 aagatcgatg caggtaaccc ccttcaccgc ttcctcacgc atttcccaaa gcggtagcag
    49621 aacgtcctga tccagctctc cggataacgc cagcgtgtca cccgtctgca tccagctcag
    49681 tgactcgctc attatttttt ctcttccaga gtgattttct gttgagaaat cgatttcagt
    49741 tgcgcagtca ggccgtcgat acctttggta cgcagcagcg ttccccactc gttttgtttg
    49801 gtggtgatca tactgacgcc ttcagcaatc atgtcgtaag cctgccagtt gcccgtctgg
    49861 gagtttttac gccactggaa gtccagacgc accggcgggc ggccattcgg gtcaataatg
    49921 gtaacgcgaa taggcacaat ggttttatca cccagcggct gttctggcgc aatctgatag
    49981 gtttgaccgt gatacatcgc cagcgcctga ccgtaagcct gcttcaggta ctcacggaaa
    50041 gcggcaaagt aggcttcacg ttgagctggg gtcgcactct tgtaatactg gcccagcacc
    50101 agcgcaccgg cgtatttcac ctgtacgtat ggcagcagtt cctgatcaac aatggtacgc
    50161 agataatccg ggttggcccg aatttgcggt tgctcattct tcaggcgatc gaacgttttc
    50221 tgcgccgcct cgtccatcag cttatacgga ttggtctggt ctgccgcggt tgccgcactc
    50281 agcggtgcaa tcaccagcaa agcgaccatc attaaacgtt taaacatgcg tcggttctcc
    50341 tgaaattatt tcgttgtacc cacaggttca gtggtttcat tattacctgg cgcagcagct
    50401 ggcgcatcgc cactattctt attgtcatcg cctttactac cgtaaaggaa ctgaccaatg
    50461 agatcttcca gcaccatcgc agacttggtg tcctgaattg tatcgccatc cttcaggata
    50521 gcagtcccca gttccgggtc ttcaaaaccg acgtttaatg ccagatattg ttcccccagc
    50581 aggccggaag tacgaatgct cagcgaactg gtatcaggaa tatggttata acgttgttca
    50641 atttccagcg ttacgcgcgg cagataggtt ttcgggtcca gcgtaatatc cgccacccgg
    50701 cccacaacaa cgccaccaat actgaccgga gagcgcgctt tcaggccgcc aatgttatcg
    50761 aacgtcgcat aaagcgtata ggtcggttca gtacgtattg acgtcacgtt cgccgccttc
    50821 aggcaaacaa acagcgccgc cagcagtgct gctaataaaa agatacccac ccaaatttca
    50881 ttttttttcg tttgcatgaa ctcaattccc aaacatcaat gcggtcagca caaaatccag
    50941 ccccagaaca gccagagacg agtggacaac ggtgcgagtg gttgcccggc taatcccggc
    51001 agacgtcggg atggcgtcgt agccgttaaa caacgaaatc cacgtcaccg tgatggcgaa
    51061 caccacgctc ttaatcagac agttgaccag atccatacgc cagtcgacgg cattttgcat
    51121 tgccgaccag aagaacccgc tatcaatgcc tttccaactg acgccgacca gcgatccgcc
    51181 ccagatcccc acggcgacga agataaccgt caacagtggt aatgaaataa ccccagccca
    51241 gaaacgggga gaaataaccc gacgcagcgg atccaccgcc atcatctcca tactggagag
    51301 ttgctctgta gcgcgcatca ggccgatttc tgcggttaac gccgaaccag cacgcccggc
    51361 aaacaacagc gcggcaacca ccggccccag ttcacgcagt agcgataacg ccaccagcat
    51421 acccagactg gtttccgcac tataagtggt cagaaccaga taaccttgca gccccaacac
    51481 cattccgatg aacacgccag aaactacaat aatcagcatc gacaggacgc cgacattata
    51541 gagctggcgc accagcagcg gcgcatgttt gcgaaattcc ggtttgccga ccagcgcatt
    51601 gaataacatt aacccggccc gcccgaacgt tctcagggtt ttaatccctt tatgtccgag
    51661 cgacgccagc gcatttaaca gcatgagtgg cttaactccc tggtaaaaga tcagcgtgat
    51721 aatcgccggc aggatagcgg aacggaacag gcccgtcagc tatcccgtcc agaaactgac
    51781 gtacgcgcgg atcaggattc gcctgcaacg ccttggcact gccatgagcg acaatttttt
    51841 tgtccgccag gatccaggcg tgatccgcaa tacttaacac ttccggcaca tcgtgagaaa
    51901 ccaccacaca agtcacgccc agcgcgctgt tcagctcaga aatcagcttc accagtacgc
    51961 ccatggtaat gggatcttgc ccaacaaagg gctcatcaaa catgatgaga tccggctcca
    52021 gcgcaatcgc acgcgccagc gctgcacgcc gcgccatccc accggaaagt tcagaaggca
    52081 ttagtttagc cgctccacgc agccccacgg cctccagctt catcatcacc gtactatgca
    52141 acaatggcgc gggaagttgg gtatgttcgc gcagtggata ggcgacgttg tcaaatacgt
    52201 tcatatcagt gaacaacgcc ccggactgaa ataacatgct catccgtttg cgcactgtat
    52261 acaggcgcga acgagacatc gccggaatat tctcaccatc gaaaaggatc tcaccatgat
    52321 ctggtgcgat ttgcccgcca atcagacgga gtagcgtcgt tttaccgatg cccgatggcc
    52381 ccatgatcgc cgtgatcttc cctcgcggca cggtcaggga aatattatcg aagatgcagc
    52441 gattgccacg cgtaaaactg acatcgcgca tatcgactaa attcgccaca gactgctcca
    52501 taattcaccc ttcgtcttgc gttgattttc taagcatggc gctcaattta accttgaacc
    52561 caacatattt acagaatatt acccgccgtg gttagcgaaa gctggcattt gttttacttt
    52621 ttagccgcat aaagtcaaaa ttaagaatcc gttacggctt tctgaaaatc ttcagcggac
    52681 cggcgagtat acctgaagaa aggacgttag atgcttttag ctacggcact gttaattgtt
    52741 ggtttacttt tggtcgttta cagtgccgac cgcctggttt ttgccgcgtc tattctttgc
    52801 cgaacctttg gcatcccgcc gctgatcatc ggcatgacgg tggtcagtat tggtacatcg
    52861 ttaccagaaa tcatcgtctc gcttgccgcg tctctgcacg aacaacgcga tttagccgtt
    52921 ggtacagccc tcggctcaaa cattatcaat atattgctga tcctcggtct ggctgcgctg
    52981 gttcgtcctt ttaccgtcca ttctgatgtt ctacgccgtg aattaccctt aatgttgttg
    53041 gtcagcgtgg tggccggttc cgtactctat gacggacaac ttagtcgcag cgatggtatc
    53101 tttctcctct ttctggctgt gctatggctg ctgttcattg ttaaacttgc acgtcaggct
    53161 gaacgtcagg ggactgacag cctgaccaga gagcagcttg cagagctgcc gcgtgacggc
    53221 ggattgcccg tcgcgttttt atggctcggc attgcgctta tcatcatgcc agtggccacg
    53281 cggatggtgg ttgataacgc cacggtgctg gcgaattact ttgccatcag cgagttgacg
    53341 atgggtctga cggcaattgc tatcggaacc agcctgccgg aactggcaac cgcaatcgcg
    53401 ggggttcgca aaggtgaaaa cgacattgct gtcggaaata tcattggcgc aaacattttt
    53461 aatattgtca tcgtgttggg tttacccgcg ctgataacgc caggagagat tgatccactg
    53521 gcgtacagtc gtgactacag cgtgatgttg ctggtgagca ttatttttgc gttgctgtgc
    53581 tggcggcgct ccccgcaacc gggccgtggt gtaggggtat tattaactgg cggatttatc
    53641 gtatggctgg cgatgttgta ctggttatcg ccaatactcg ttgaataact ggaaacgcat
    53701 tatgtcgcac gtagagttac aaccgggttt tgactttcag caagcaggta aagaagtcct
    53761 ggcgattgaa cgtgaatgcc tggcggagct tgatcaatac atcaatcaga atttcacgct
    53821 tgcctgtgaa aagatgttct ggtgtaaagg gaaagttgtc gtcatgggga tggggaaatc
    53881 ggggcacatc gggcgcaaaa tggccgcaac gtttgccagc accggtacac cttcattttt
    53941 cgtccatcct ggtgaagccg cgcatggtga tttaggcatg gtcaccccac aggatgtggt
    54001 gattgctatc tctaactcag gtgaatccag cgaaatcact gccttaattc cagtgcttaa
    54061 gcgtcttcac gtaccgttaa tctgcatcac cggtcgcccg gagagcagca tggcgcgcgc
    54121 cgcagatgtg catctgtgtg ttaaagtagc gaaagaagcc tgtccgttag ggctggcacc
    54181 gaccagcagc accaccgcca cgctggttat gggcgatgcc ctcgctgtcg cgctgttaaa
    54241 agcacgcggc tttactgctg aagattttgc gctctcacac ccaggcggcg cactgggtcg
    54301 taaacttctg ctgcgcgtaa acgatattat gcatacgggc gatgagatcc cgcatgttaa
    54361 gaaaacggcc agtctgcgtg acgcattgct ggaagttacc cgcaaaaatc ttggtatgac
    54421 tgtcatttgc gatgacaata tgatgattga aggcatcttt accgacggtg atttacgccg
    54481 tgtcttcgat atgggcgtgg atgttcgtca gttaagtatt gccgatgtga tgacgccggg
    54541 gggaatacgc gtgcgccctg gcattctggc cgttgaggca ctgaacttaa tgcagtcccg
    54601 ccatatcacc tccgtgatgg ttgccgatgg cgaccattta ctcggtgtgt tacatatgca
    54661 tgatttactg cgtgcaggcg tagtgtaaag attcaaggat aaacaacaat gagcaaagca
    54721 ggtgcgtcgc ttgcgacctg ttacggccct gtcagcgccg acgttatagc aaaagcagag
    54781 aacattcgtc tgctaatcct cgacgttgat ggcgtactgt cagatggcct gatttatatg
    54841 ggcaataacg gcgaagagct gaaagcgttc aatgttcgtg acggttatgg cattcgttgt
    54901 gcgctcacct ctgatattga agtcgctatc attaccgggc gaaaggctaa actggtagaa
    54961 gatcgttgtg ccacattggg gatcactcac ttgtatcagg ggcagtcaaa caaactgatc
    55021 gcctttagcg atctgctgga aaaattggcg attgccccgg aaaatgtggc ttatgtcggc
    55081 gatgatctca tcgattggcc ggtaatggaa aaagtgggtt taagcgtcgc cgtggccgat
    55141 gcgcatccac tgttgatccc gcgcgccgat tacgtgacgc gcattgctgg cggtcgtggc
    55201 gcagtgcgag aagtttgcga cttattactc ctggcgcagg gcaaactgga tgaagccaaa
    55261 gggcaatcga tatgagtaaa gccagacgtt gggttatcat tgtgctatca ctggcggttc
    55321 tggtgatgat cggtattaat atggccgaaa aagacgatac cgcccaggtg gtcgtcaaca
    55381 acaatgatcc cacctataaa agcgagcata cggacacgct cgtctataac ccagaagggg
    55441 cgctaagcta tcgattgatt gctcaacacg ttgaatatta ttccgatcag gccgtttcgt
    55501 ggtttacgca gccggtactt accacgtttg ataaggataa aatcccgaca tggtccgtaa
    55561 aagcagataa agccaagctg accaatgacc ggatgctcta tttatatgga cacgttgaag
    55621 tcaacgcact tgtgccggac tctcaacttc gcagaatcac gacggataac gcgcagatca
    55681 atctggtgac gcaggatgtt acctctgaag acctcgtcac gttatacgga acaacattta
    55741 actccagcgg tctgaaaatg cgcggcaact tacgcagcaa gaacgccgag ctgattgaaa
    55801 aggttagaac atcctatgaa attcaaaaca aacaaactca gccttaatct tgtgcttgcc
    55861 agctcacttc tggccgccag cattccggca tttgccgtaa ccggagacac tgatcagccg
    55921 atccacattg aatcggacca gcaatctctt gatatgcaag gcaacgtggt tacctttacc
    55981 ggtaatgtca tcgtcaccca gggcaccatc aaaattaatg ccgacaaagt ggtcgttacc
    56041 cgtccgggcg gcgaacaagg taaagaagtg attgacggct acggtaaacc ggcaacgttc
    56101 taccagatgc aggacaacgg taaacccgtt gaaggtcacg cttcccagat gcactacgaa
    56161 ctggcaaaag attttgtcgt tctgacgggt aatgcttatc tgcagcaggt cgatagcaac
    56221 attaagggcg ataagatcac ttacctggtg aaagagcaga aaatgcaggc tttcagcgac
    56281 aaaggcaagc gcgtaaccac cgttctggta ccgtcgcagc tgcaggacaa aaacaacaaa
    56341 ggccagaccc cggcacagaa gaagggtaat taattcgtta tggcaacatt aactgcaaag
    56401 aaccttgcaa aagcctataa aggccgtcgc gtggtagaag acgtcagcct gaccgtcaac
    56461 tccggggaaa ttgtcggtct gctggggcca aacggtgccg gtaagaccac cactttctac
    56521 atggttgtag gcattgtgcc gcgcgatgcg ggcaacatca ttattgatga tgacgatatc
    56581 agtctgctgc ctctgcatgc acgcgcgcgc cgcggtatcg gctatctgcc acaggaagcc
    56641 tccattttcc gtcgcctcag cgtttacgat aacctgatgg cggtactgca aattcgtgac
    56701 gacttgtctg ctgaacaacg tgaagaccgc gcgaacgagc tgatggaaga gtttcacatt
    56761 gagcacctgc gtgacagcat ggggcagtca ctctccgggg gtgaacgtcg ccgtgtagaa
    56821 attgcccgcg cactggctgc gaatccgaaa tttattctgc tcgacgaacc gtttgccggg
    56881 gttgacccga tctcggttat cgacattaaa cgcatcattg agcacctgcg cgacagcggc
    56941 ctgggcgtgc tgatcactga ccacaacgtg cgtgaaacac tggcggtttg tgaacgcgct
    57001 tatatcgtca gtcaggggca tttgatcgcc cacggcacgc ctacagaaat cttacaagac
    57061 gaacacgtta agcgtgtata ccttggggaa gacttcagac tctgataggg tagaagtttg
    57121 cgacgtttta gcaggagagt acgattctga acatgaagca aggtttgcaa ctcaggctta
    57181 gccaacaact ggcgatgacg ccacagcttc aacaggcaat tcgtctgttg cagttgtcga
    57241 cgctggaact tcagcaggag ctacagcagg cgctggagag taatccgctg cttgagcaaa
    57301 tcgacactca tgaagaaatc gacacccgcg aaacgcaaga cagtgaaacg ctggacaccg
    57361 ccgacgcgct cgaacaaaaa gagatgccgg aagagctgcc gctcgatgcc agttgggata
    57421 ccatttacac cgctggtaca ccatccggca ccagcggtga ctacattgac gacgagctgc
    57481 cggtctatca gggcgaaacg acgcagacct tgcaggatta cctgatgtgg caggtcgagc
    57541 tgacaccgtt ttccgacact gaccgcgcta ttgctacctc tatcgtcgat gccgttgatg
    57601 acaccggtta tctgactgtc ccgctggaag atattctcga aagtatgggc gatgaagaga
    57661 tcgacatcga cgaagttgaa gccgtcctta agcggatcca acggtttgat ccggtcggtg
    57721 tggcggcaaa agatctgcgt gactgcctgc tgatccaact ctcccaattc gataaaacca
    57781 cgccgtggct ggaagaggcc agactgatca ttagcgatca tctcgatctg ttagccaatc
    57841 acgacttccg cactttaatg cgcgtcacgc gtctgaaaga agatgtgctg aaagaagccg
    57901 tcaatctgat ccagtcgctc gatccgcgcc ccgggcaatc gatccagact ggcgaacctg
    57961 agtatgtcat tccagatgtg ctggtgcgta agcataacgg tcactggacg gtagaactca
    58021 acagtgacag cattccgcgt ctgcaaatca accagcacta cgcctcgatg tgcaataacg
    58081 cgcgcaacga tggtgatagc cagtttatcc gcagcaatct gcaggatgcc aaatggttga
    58141 tcaagagtct ggaaagccgt aacgatacgc tactgcgcgt gagtcgctgt atcgttgaac
    58201 agcagcaagc cttctttgag caaggtgaag aatatatgaa accgatggta ctggccgata
    58261 tcgcccaggc tgtcgaaatg catgaatcga cgatatctcg cgtgaccacg caaaaatacc
    58321 tgcatagtcc acgaggcatt tttgaactga agtatttctt ttccagtcac gtcaataccg
    58381 agggcggcgg cgaagcttcc tccacggcga ttcgtgcgct ggtgaagaaa ttaatcgcgg
    58441 cggaaaaccc agcgaaaccg ttgagcgaca gcaagttaac ctctttgctg tcggaacaag
    58501 gtatcatggt ggcacgccgc actgttgcga agtaccgaga gtctttatcc attccgccgt
    58561 caaaccagcg taaacagctc gtttgaccca accgataagg aagacactat gcagctcaac
    58621 attaccggaa ataacgtcga gatcaccgag gcactgcgcg aatttgttac agccaaattt
    58681 gccaaacttg agcaatattt tgaccgaatc aaccaggtct atgttgttct gaaagtggag
    58741 aaagtcaccc acacctcaga tgcaacactg catgtaaacg gtggtgaaat tcatgccagc
    58801 gcggaaggtc aggacatgta cgctgccatt gatggcttaa ttgataagct ggcacgccag
    58861 ttgaccaaac ataaagataa actgaaacaa cactaattgt ccgggcaatt agcatgtgca
    58921 tggcggcctg ttgtgcggca caacgggcca tttgtacggt taatgctccg agcctgttcc
    58981 actgtttgag tgggcaggtt cttaggtgaa attatgacaa ataatgatac aactctacag
    59041 cttagcagtg ttcttaacag ggaatgtacg cgaagccgcg tccactgtca gagcaaaaaa
    59101 cgcgccctgg aaatcatcag cgagctggcg gcgaaacaac ttagcctgcc gcctcaggtg
    59161 gtttttgaag ctatcctgac gcgggaaaaa atgggcagta ccggtatcgg caatggtatt
    59221 gccattccgc atggcaaact ggaagaagat actctgcgtg ccgttggcgt tttcgttcag
    59281 ctcgaaacgc ctatagcttt cgatgccatc gacaaccaac cggtggactt actttttgcc
    59341 ctgctggtgc cggcagacca aactaaaacg cacctgcata ctctgtcgct ggtggcgaaa
    59401 cgtctggcgg acaaaaccat ctgccgccgt ttacgcgcag cccagagcga tgaagagctg
    59461 tatcaaatca ttacggatac cgaaggtact ccggatgaag cgtagttatt cggtaatgtc
    59521 tcttttagac gttgtgagga gaaacagtac atggtactga tgatcgtcag cggacgttca
    59581 ggttcaggta aatctgtcgc cctgcgtgcg ctggaagata tgggttttta ctgcgtggat
    59641 aaccttcccg tagtgttgtt acccgatctg gctcgaaccc tggccgatcg cgagatttct
    59701 gccgccgtca gcattgatgt tcgtaatatg ccggagtcac cagaaatatt cgaacaggcg
    59761 atgagtaacc tgcctgacgc tttctcaccg caactcctgt tcctggatgc cgaccgtaat
    59821 accttaattc gtcgttacag tgacacgcgc cgactgcatc cgctttccag caaaaacctg
    59881 tcgctggaaa gtgctatcga caaagaaagc gatctgctgg agcctctgcg ttcgcgagcg
    59941 gatctgattg tcgatacctc agaaatgtcc gttcacgagc tggcagaaat gctgcgtacc
    60001 cgtctgctgg gtaaacgtga acgcgaactg acaatggtct ttgagtcttt cggcttcaaa
    60061 cacggtatcc ctatcgatgc agattacgtc tttgacgtgc gcttcttgcc gaacccgcac
    60121 tgggatccga aactgcgtcc aatgacaggt cttgataagc ctgtcgccgc gttcctcgac
    60181 cgccacacag aagtacacaa ttttatctac cagacgcgaa gctatcttga gctatggtta
    60241 cctatgctgg aaaccaacaa ccgtagctac ctgacggtcg ccattggttg taccggcggg
    60301 aagcaccgtt cggtgtatat tgcagagcaa ctggctgact acttccgctc gcgcggtaaa
    60361 aacgtccagt cacgccatcg tacgctggaa aaacgtaaac catgaccgtc aagcaaactg
    60421 ttgaaatcac aaacaagctg ggcatgcatg cccggcctgc aatgaagctg tttgaattaa
    60481 tgcagggttt tgacgctgaa gtgctgttac gtaatgacga aggcaccgag gctgaagcca
    60541 acagcgttat tgcgctgctg atgttggatt ctgccaaagg acggcagatt gaagttgaag
    60601 cgaccggtcc acaggaagag gaagcactgg ccgccgttat cgccctcttt aattctggtt
    60661 ttgatgaaga ttaatcttca tcactttgac atacaactcc cttcaaactc cccccgacaa
    60721 taagaaaatc acgtactgaa atcgttctca atcaacgtca attgtacatt tcgtgcgctt
    60781 tttactttca gaagaacctt aagaaaacct taagaagcat tgtttaggtt tcgtttaagt
    60841 taatcgacca tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat
    60901 ctgaacaaag tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg
    60961 cccaacgctg tatcaagatt gtctaccatc gtggcgatgg tggcgataaa gagatccgcc
    61021 gtgagttaaa gtactacgcg catcttggtc gccggttaaa agactggagt ggaataccgc
    61081 gctatcacgg taccgttgag acggattgcg gaaccggtta tgtctacgat gtgattgccg
    61141 attttgacgg caagccctcc attacgctga ccgaatttgc tgaacaatgc cgttacgaag
    61201 aagacgttgc acaactgcgc caattactga aacagctgaa gcgttatttg caggataacc
    61261 gtatcgtgac gatgtcgctg aagccgcaga atatcctctg tcaccgcatt agtgagtcag
    61321 aggttatccc cgtcgtctgc gacaacattg gtgaaagaac attgattcca ctggcgacct
    61381 ggtcaaagtg gtgttgtctg cgtaagcaag agcggttgtg gaaacgattt atcgcccaac
    61441 cggcgctggc tattgccctg cagaaagatc tgcaacctcg tgagagcaaa acgctggccc
    61501 tcacttcgcg cgaagcttaa tccagctggt gttgttgcat aaacggctca ccgcctaact
    61561 gatacatctg ccgtaaaatc cacgcctgac ggctgcgcac gtagcccgat ggtgcggaga
    61621 ctttgaaacg aagtggatta ggtaatacag ctgccagtaa ggcagcttcc gatcgggtaa
    61681 gtttgctcgc gggtttgtgg aaatagcgtt gtgccgcagc ttcgacgcca aacacaccgt
    61741 cgccaaattc ggcgatattc aggtaaaccg tcaggatacg ctttttgctc cagactgttt
    61801 ctatccccag cgttaatccg gcttccagcc cttttcgaac ccagctacgc ccatcccata
    61861 aaaagagatt tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat
    61921 tgcgctcgtt gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagcgctcag
    61981 gaaatttctg atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca
    62041 tactgaccca gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct
    62101 gtcgctcgac cattaccgct gagaaaggaa caggcgcaac gctaaacaac gcgatgcccc
    62161 cgccccagaa aacggcgagg acaaccatta accgcagaag aaaacggcga acgaaactaa
    62221 acaccgttaa gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc
    62281 aatgccgctc gccgcttctg caatgttctg cgccagcata tatgccgggg tggtgacaat
    62341 cttattgtct tcatcaacca cgatatcatc gacaggacac ggcacatgct ctgcgcccat
    62401 ctcttccagc acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc
    62461 gaaaattttc ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg
    62521 atgcatcgct tgtgccagcg cctttaattc acggtcaacg gtgcattcgc gaccaagact
    62581 ggcaaaattg cttaaattct ttgccgcgcc aaaccccccc ggcacaatca acgcatccag
    62641 ttcagcggca tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc
    62701 aatcagcaca ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac
    62761 ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa
    62821 cgtcaacacc gcttcatgaa tttcagaacc gtcatagaca ccgcatccgc tcagaattac
    62881 gccaattttc ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta
    62941 ataaaaatgg tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc
    63001 tatcttaact gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat
    63061 ttacggcgca gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc
    63121 ggggtcattt tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc
    63181 actcttcttt catctcttca atccattcac caacgttatc ttcccaggca ggaaggtcag
    63241 gagactgaat ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac
    63301 ctttaatttt atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact
    63361 ccagcacgct gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca
    63421 gcttcggtcc tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg
    63481 atttactgtt ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt
    63541 tcttgatcat cgcggttagc gccggaacag aaagcggctt actcagcaca tcatccattc
    63601 cagcattgag gtactcttgc ttgtctttca gcacgttagc ggttaaagcc accagcggcg
    63661 gtaaatcctc gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat
    63721 ctggcaactg aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg
    63781 ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg
    63841 cgacaatcac gttcagttca atgtcttcca ccagtagcac attcagcgcc ggtaaaggca
    63901 tatcgtcttc atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca
    63961 acgtaaaggt tgaacctttg ccctgttcgc tggtaaccgt aatatcgccg cccatatttt
    64021 tcgccagacg acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat
    64081 gactgtcttt cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc
    64141 cgataccaga atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca
    64201 cggtaacctg gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct
    64261 gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt
    64321 taaagcgcaa tcctttttgt tgcgcctgca aggcggagag attttccaga tcggcaagga
    64381 agctggtgaa atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca
    64441 tgtcgataat atcgttgaag atattcccca gcgtgacggc cgaaacatgg atagttttga
    64501 gatatttttc ctgctcggcg gtgagttcgg tatccagtag gatgcggctc aggccgacga
    64561 taccgttcag cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc
    64621 ggctggcgcg ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc
    64681 ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg cactttacga atttcaaagc
    64741 aggctttgcg tccgtcggga taatccagcc actgctcata ggtcagtgac acattatgac
    64801 ggaacacttt ttcatcggtt tcaatgactt tcgcagcggc ttccggtgag taaacatcag
    64861 caggtttcag atgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt
    64921 tacagccgga aaactcttta tcttcgttac gataaaaaac caggtcgggt gaagcatcaa
    64981 ggaaggaacg taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt
    65041 tgatttcaat tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa
    65101 cggcaatttc ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat
    65161 cgcgctcgcg catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct
    65221 ccagttgctc gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa
    65281 agaagataga acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg
    65341 ccatttgtac cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca
    65401 ccagacctaa cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca
    65461 ttagggaatt ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat
    65521 aaattgtgtg agaaatgcgg aattcccctc aatgacctac tccgtaggag gtagccacgg
    65581 acgtcccagc gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat
    65641 tcccgtccat cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc
    65701 ggaaatgcgg tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag
    65761 tgtgtaatcc tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat
    65821 gctgcctttc acaatatgca gcggatgcag ctttatgccg tccacgccgg tttcaaccac
    65881 gcgctccagc gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg
    65941 ggaacacacc ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa
    66001 atcatggccg cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc
    66061 cagccacact tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg
    66121 cacgcagtcc gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg
    66181 ctgatacata gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta
    66241 ggccagatag cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat
    66301 ggaacgatgt tgctgcgctt catcagcaaa cgaggcaaca ttacagaatg tgcagccgcc
    66361 acgcccgatg gtaccgtcac ggttagggca gctaaaaccg ccatgcagcg tcagcttatg
    66421 caccttttgc ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa
    66481 ctgcataatc tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcat
    66541 ttcggcgatg acctggatca atcgtcccgg cctgccttta ccaactgcat aatcaatcaa
    66601 aattaccgaa atttcatgca taatcacata aatcactttt gcttatcttg tgtcagattt
    66661 ttttatctcc ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag
    66721 gcagtataaa atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt
    66781 atgacagtca ggaattggct gtttctctaa cgacgctcct tttatcctta attagaaaat
    66841 ctattttaat ttcagtcaat taataaagaa ttttgcgcta aagcacattt ctgtaccaat
    66901 aagcttgcca tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt
    66961 ctggatgagc agcctgctca ttatatttat gcagtaattg agatcccctc ttcaccgtat
    67021 taatcgatgc gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac
    67081 cccgtcgcca cgctctttct gtgcccgtgc gcagcggttc ggtagtgggg ttcccgcaga
    67141 gcctggggga ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt
    67201 tcggcctgat cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac
    67261 acgcactggc ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggtgacg
    67321 gttgcggctt gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg
    67381 gctggcgttt agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac
    67441 tcgctgctgc cgcacgccgc atcgttgaag aagagctgca acgcgaaacg ttgtcgattg
    67501 tgggctggcg tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc
    67561 tgccacgcat tgagcaaatt tttgtgaacg ccccggcagg ctggcgtccg cgtgatatgg
    67621 aacgccgtct gtttatcgcc cgccgccgca tcgaaaagcg tctcgaagcc gacaaagact
    67681 tctacgtctg tagcctgtcg aatctggtca atatttataa aggtctgtgt atgccggcgg
    67741 atctgccgcg cttttacctg gatcttgcgg acctgcgcct ggaatcggcc atctgcctgt
    67801 tccaccagcg cttctccact aacaccgtac cgcgctggcc gctggcgcag ccgttccgct
    67861 atctggcgca taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc
    67921 gtacctataa attccagaca ccgcttatcc ctgacctgca cgacgccgca ccgttcgtca
    67981 acgaaaccgg ctctgactcc agttcgatgg ataacatgct ggaactgctg ctggcaggcg
    68041 ggatggatat catccgcgcc atgcgtctat tagtaccacc cgcctggcag aacaacccgg
    68101 atatggaccc ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg
    68161 atggcccggc gggcatcgtg atgtctgacg gtcgttttgc tgcctgtaac ctcgaccgta
    68221 acggtctgcg tccggcgcgc tacgtcatca ccaaagataa gctcatcacc tgcgcctctg
    68281 aagtcggtat ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag
    68341 gcgaactgat ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg
    68401 acgatctgaa aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg
    68461 taccgtttga agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc
    68521 ttgccagcta ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg
    68581 tactgggcga aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg
    68641 tgctctccag tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga
    68701 ctaacccgcc aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagcattg
    68761 gtcgtgaaat gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat
    68821 cgccgattct gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc
    68881 gcgcagatac gctggatatc acctttgacg tcaccaaaac cacgctcgaa gcgacggtca
    68941 aagagctgtg cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct
    69001 ccgaccggaa tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga
    69061 tccagacccg tctggtcgat caaagcctgc gctgcgatgc caacatcatc atcgaaaccg
    69121 ccagcgcccg cgatccacac catttcgccg tgttgctggg cttcggtgcg acggctattt
    69181 atccgtacct tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag
    69241 attatcgtac cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca
    69301 tgtccaaaat gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg
    69361 tcggtctgca cgatgatgta gtgggcctgt gcttccaggg ggcggtcagc cgcattgggg
    69421 gggcgagctt tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg
    69481 cgcgcaagcc catcagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg
    69541 cctacaaccc ggacgtggtg cgcacgctgc aacaggcggt acagagcggc gagtatagcg
    69601 actatcagga atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc
    69661 tggcaattac gccgggtgaa aacgcggtca acattgcgga tgttgaaccg gcaagcgaac
    69721 tgtttaaacg cttcgatacc gctgcgatgt ctatcggcgc gttaagcccg gaagcccacg
    69781 aggcactggc ggaagcgatg aacagcatcg gcggtaactc taactccggt gaaggtggtg
    69841 aagacccggc gcgctacggc accaacaaag tgtcgcgcat caagcaggtg gcttccggtc
    69901 gctttggggt tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc
    69961 agggcgcgaa gccgggcgaa ggcggtcagt tgccgggcga caaagtcact ccttacattg
    70021 ccaaactgcg ctattcggtg ccgggcgtga cgctgatctc cccgccgccg caccacgata
    70081 tctactctat cgaagactta gcgcagctca ttttcgacct caagcaggtt aacccgaaag
    70141 cgatgatctc cgtgaagctg gtttccgaac cgggcgttgg taccatcgcc accggtgtgg
    70201 cgaaagctta tgccgattta atcaccatcg caggctatga cggcggcacc ggcgcaagcc
    70261 cgctgtcgtc agtgaaatac gcaggttgcc cgtgggagct ggggcttgtt gaaacccagc
    70321 aggcgctggt tgccaatggt ctgcgtcata agatccgttt gcaggtcgat ggcggcctga
    70381 aaacgggtgt cgatatcatc aaagcggcga ttctcggcgc ggaaagcttc ggcttcggca
    70441 ctggcccaat ggtggcgctc ggttgtaaat atctgcgtat ttgccacctg aacaactgcg
    70501 ccacgggtgt agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt
    70561 tcaaggtgac gaattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc
    70621 ttggcgtaac acgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg
    70681 gtttcaccgc caaacagcag aaactggcgc tgtcgaagct gctggagacc gccgaaccgc
    70741 atccaggtaa ggcactctac tgcaccgaaa ccaacccgcc gtttgataac ggcctgctga
    70801 acgcgcagtt gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaactttct
    70861 ggttcgatat ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc
    70921 agacgcacgg cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg
    70981 caggccagag tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg
    71041 ccaacgacta tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg
    71101 gttccgcctt ccgcagccat gaagcaagta ttatcggcaa cacctgcctg tatggcgcga
    71161 ccggtggtcg tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cgtaactccg
    71221 gtgcaatcac cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta
    71281 tcgtctgtat tctgggtaaa accggcgtta acttcggtgc gggtatgacc ggcggcttcg
    71341 cttacgttct cgatgaaagc ggcgatttcc gcaaacgcgt taacccggaa ctggtggaag
    71401 tattaagcgt tgacgatttg gcgatccatg aagagcatct gcgtggtctt atcaccgagc
    71461 atgtgcagca taccggttcc cagcgtggtg aagagattct ggcgaactgg tcagccttcg
    71521 ccactaaatt tgcgctggtt aaaccgaagt ccagtgatgt caaagcactg ctgggtcacc
    71581 gtagtcgtag cgcagcagag ttgcgcgtgc aggcgcagta aggggtagct acaatgagtc
    71641 agaatgttta tcaatttatc gacctgcagc gcgttgatcc gcctaagaaa ccgctgaaga
    71701 tccgcaaaat tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc
    71761 aggctgaccg ctgcctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca
    71821 actacatccc gaactggctt aagctcgcca acgaggggcg tatttttgaa gcggcagaac
    71881 tgtcgcatca gaccaacact ctgccggaag tatgcggacg tgtctgcccg caagaccgtc
    71941 tgtgcgaagg ttcctgcacc ctgaacgatg agtttggcgc ggtgaccatc ggcaacattg
    72001 agcgttatat caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga
    72061 aacagaccgg taaaaaagtg gcgattatcg gcgcaggtcc ggcaggtctg gcgtgtgcag
    72121 atgtcctgac gcgcaacggc gtacaagcgg tcgtctttga ccgtcacccg gaaattggcg
    72181 gcttgctgac cttcggtatt ccggctttca aactggaaaa agaggtaatg acgcgccgcc
    72241 gtgaaatctt caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg
    72301 tacagctgga cgatctgctg agtgattacg atgccgtgtt ccttggcgtc gggacttatc
    72361 agtcaatgcg cggcgggctg gaaaacgaag acgctgatgg cgtgtacgca gcgctgccgt
    72421 tcctcatcgc caacaccaaa cagttaatgg gctttggtga aaccaacgac gaaccgttcg
    72481 tcagcatgga aggtaaacgc gtggtagtac ttggcggtgg cgataccgcg atggactgcg
    72541 tgcgcacgtc tgtacgccag ggagcgaagc acgtaacctg tgcctatcgt cgtgatgaag
    72601 agaacatgcc aggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggt gttgagttca
    72661 aattcaacgt ccagccgctg ggtattgaag tgaacggcaa cggcaaagtc agcggcgtaa
    72721 aaatggtgcg taccgaaatg ggcgaaccgg acgccaaagg ccgtcgccgc gcggagatcg
    72781 tggctggttc cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc
    72841 cacacagcat ggaatggctg gcaaaacaca gcgtcgagct ggattcgcaa ggccgcatca
    72901 tcgccccgga aggcagcgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg
    72961 gcgatatcgt ccgtggttcc gatctggtcg ttaccgctat tgccgaaggt cgtaaggcgg
    73021 cagacggcat tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa
    73081 aagagtcggc gctgaaattc tcctttcgtt ctggaaagcg cctcgcataa tctgaagaaa
    73141 accaatgtaa tacgcacatc tggattgtta ccggcagatg cccggcgtat agctgattcc
    73201 attattcaac actgcgccat ataaagggaa agataatgga atcgctctca gagagaacct
    73261 caacaggcta ccagcaaatc cacgacggca ttattcatct ggtcgatagc gcccggacgg
    73321 aaacggtacg tagcgttaac gcgctgatga ccgcgacata ctgggaaatt ggccgacgaa
    73381 ttgtcgaatt tgaacaaggt ggcgaggcca gggctgcgta tggtgcgcag ctaatcaagc
    73441 gactatcaaa ggatttaagt ctaaggtata agcgtgggtt ctctgcaaaa aacttacgcc
    73501 aaatgaggct tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg
    73561 aattaacacc attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt
    73621 ggcagacgct gtctgccaaa tctttccccc tgccctggtc cacctacgtc cgtttacttt
    73681 ccgttaaaaa cgctgacgcc cgcagccttt atgaaaaaga gacgctccgc tgtggctggt
    73741 ctgttcgcca gctagaacgg caaattgcga cccaatttta tgagcgaaca ctgctgtcac
    73801 atgacaaatc agccatgctg caacaacacg ctcctgccga gacgcatatt cttccgcaac
    73861 aggcgatacg cgatcccttt gtgctcgaat ttctggaatt gaaagatgaa tactcagaat
    73921 ccgattttga ggaggcgctg atcaaccacc tgatggattt catgctggaa cttggggatg
    73981 attttgcctt tgttggtcgg cagcgaaggt tacgcattga tgacaactgg tttcgtgtcg
    74041 atctgctgtt tttccaccgc cgtttacgct gcctgctaat cgtcgatcta aaagtgggca
    74101 aattcagcta tagcgatgcc ggacagatga atatgtatct caactacgcc aaagagcact
    74161 ggacgctacc ggatgaaaat ccgcccatcg gtctggttct ctgtgcagag aaaggagtcg
    74221 gagaagcgca ttatgcactg gctggtttgc ctaacaccgt tctggcgagt gaatataaga
    74281 tgcaactacc tgatgaaaaa cgactcgcgg atgaactcgt tcggacacag gcggtgctag
    74341 aagaaggcta tagactccgt taatttcagc ggtttcgaat tctgcagaca ctgtctgcag
    74401 aatctttaaa acgaactaag ttttaagcca ttaaatcagc cttaaccttc accacaacct
    74461 ttttaatctc accaggttcg ccgacaacgc atcctggttt atgcggttct cccggcataa
    74521 acacggcgaa cattcccggt tttaagttga tggtttgctc gttttcaatg gcgctgcaaa
    74581 gctggtaatc atcttcatgg tggaactctt cacactggcg cgcagtgcct gccgtgccaa
    74641 acagaatccg ttcctcacct tttaataaca gctggatatc aatgtattgc tcgtgcaatt
    74701 ccgctttttt ctcgacgggc gattgagtgt taaacgtcat gacattcata aaaatattgt
    74761 cgccctgtaa ttcgtaacga cccggcgctt tttcttgcgg tctggcagct aatgccagcg
    74821 ttaacgcgtc ctgtaacgca ggatgtaacc cagcagacgg taatgactgt acttcaccca
    74881 tcatcataat ttttctccct gggccaacag cgcagcccca agtaaacctg catcatggcg
    74941 gtagtgcgcc gccagtaaat caacatgaaa tgccgctggc tcctgcgcca gatacgtttc
    75001 caccagcgcc agataccctt ctgccagacc aacgctgcca ccgaccacca cgcactgaca
    75061 atcagttgtg gctttaatat cagcgatcag ccttgccagc acatgtgcgg agcggtgaat
    75121 cagctgctgc gcctgctcgt cgccctgtcc ggcgcgcatg aaaatagttt tcgcatccgc
    75181 gccagccaac tccccctgcg ctgccgctgc aataccgcga ccagaagcaa tcgcttccac
    75241 gcaacctgtg cgtccacagc cgcagactgg gccgtgtgga tcggcaagcg tatgcccgat
    75301 gtgccccgcc agaccgccag ggccggtaag cagtttgccg ccgctcacta caccgccgcc
    75361 gacgccagtg gaaacggtga taaacaccat ctcggttaca tcgccttcca gcgcctgata
    75421 ctctgcccat gctgcggcct gcgcgtcgtt aatggcaatg gtcggcaaat tggtaagttg
    75481 ttccagcgtt ttgactaacg gaaagtgtaa caatccacca agattatgcg gattcagcgc
    75541 cagcaagctg ccgtcgcgaa taatcccggt tgaagcgatg gcaacccgct gcgcatgagc
    75601 ttgcaacgga gagactaatg cggataaggc atcacgcagt gcctgcggag tctggctggc
    75661 aggcgtagga agttctcggc gatcgcggat ctgcccgtca gcgccaatca gcgcggcggc
    75721 aagtttagta ccgccgatat caatcgccag cgtggtcata gcaccgcctt tttcatcgct
    75781 gtgttgtacc actgacaaat gtgttcaaga cgcgtaatgg cagaaccgac cgtcaccgcc
    75841 cacgcgccgt ggcgcatcgc atcagccgcc tgagcaggcg tgttgtaacg cccttcggca
    75901 atcacccgac atccggcgtc gctcaacctt ttcaccagcg tcagatccgg ctcttctggc
    75961 gtttcaggcg tggtatagcc agaaagcgta gtgccaataa tttcggctcc cagcttttgg
    76021 catgccaggc cgtcttccgg cgttgagcag tcggtcatcg ccagtaaacc gtgatggtgg
    76081 atacgtgcca gcagcatttc aacaggcacc ggacgcgggc ggtcggtgcc gtcaatggcg
    76141 ataatgtccg cgcccgcctg cgccagcgca tcgacatctt caatataggc cgtgatgcgt
    76201 accggagaat cctccagatc gcgtttcaca attccaataa tcggcacgct caccaccgca
    76261 cgcgtggctt gcagatttgc cacaccttcg atgcgaatgg caaccgcgcc cgcctgctct
    76321 gccgctaatg ccatggctgc gacgatttcg ggtttatcga gcgggctgtc aggaaccggc
    76381 tggcaggaga caatcaggcc accgttagca gcgatttttt gatccagttg tgcaagtaac
    76441 gacatacatc ttcccttagc gaaaggcccg gtacatagac cgggcaacag gattaacttt
    76501 tggttttgac taaatcgttt ttggcgctgc caaatggcac ggcaccgcta aatggtttgc
    76561 cgtcgatagc gtcatgagta cgcaatgctt ccgggcgcag ccaacgctga acgcgggaag
    76621 gcatatccag accaatcagc aggatcacca caaatgtcag actgaacgag agcgatgcca
    76681 gcgcagtacc cagatccaga cgttgagcga tcaacgcgcc gatgattggg gccagtgcgc
    76741 cacccaatgc gccaacgtta taggtaaagc ccaggcccgc tgcacgctgg tcggtatcga
    76801 aataaccgcc aatcagtttc ggtaagatcc cggcgatccc ttgtccaagc atttgctgga
    76861 agaacagtaa cagaccgagc acccagacgt ttgcaccgcc aatcgcaaat accggaataa
    76921 tcagcagctg cgaggccaac agactacaaa cgtacgcttt gcgggttccc agccagtcac
    76981 cgaggaagcc accgacgcag caccccaccg ccgcgccaaa gccactaaag aacagcacat
    77041 tggcgacagt atgcgggtta taagccagat cggttttcag atacgttggc agcagcgcct
    77101 gaatcggcca tgagtagagg aaagcaaaca acacgaccac catcagcatt acgccagttg
    77161 gccagcgttt gcccgtactc tgcaccataa agctgataaa gattgcggcg cataacagcc
    77221 caagaacagc gacgatcgcg gcattttgca ggttaccggc gaagcagaac cacagcgcag
    77281 tagccgccgc cagtgtcatt acgatattgg caatgcgatg ttcaccacgg tagagaatat
    77341 ccaccattgt gcgtactggt gctttacctg cgtgtttctc tttccagtct tccgcttccg
    77401 ggatgttttt acgcagccag agagcaaaga taattggcaa aatgccgata aagaacagcg
    77461 cacgccagcc ccagaccgga accaccaggc tgtagacctg agcggcaacg acggccccca
    77521 cagagaagcc tgaaatcaga aagccactgg ctttgttacg cagatgtttt ggccagcttt
    77581 caatgacata ggtggcgctg gaaccgtatt cacccgccat ccccatgccg atgaccagac
    77641 gagcgataaa catggtgatg tagcctggcg caaagccgca ggccagcgtc ccggccgaga
    77701 agagaacgat gctggtgacc attgccagac gacgcccgta gcggtcaccc atagcgccga
    77761 gcatcaggcc gccgaaccag cgagagataa aggctgcgga gatcagactt gccgcctgca
    77821 ccgtcgtcag cccgaattca ccttgtactt cggtgagtac cagggcgatt aaaacgaaat
    77881 caaaaccgtc aagcagatat cccaaccagg cagcggaaaa tgcccgccat tgtgcacgat
    77941 tgagatggcg ataccacggg atattctggg ttgtagtact cattgtgagt ctcccgcggt
    78001 gggcgatgcc cacacgcttt ggtatgaaaa ttgtagggta cagatgcgtt tatttcccct
    78061 cacccggtag gggcgagcga ggggaaacaa ctcacccgcg ctcttgcatc aactgctggg
    78121 ccagcgcctt cagttctggc agatattttt catctaccgg accaaacggt ttgcggcaca
    78181 gcggcacaga cacgacatcc atataatgca gaacagtttt caggccgcgg aatacgcccg
    78241 ttttgatcag taaatcaatg actttattgc actcagtttg cagtttctgc gcggtctgga
    78301 tatcgccttc tttcagcgcc ttcacgatcc cctgatagcg ccagcccatg atgttgtaag
    78361 tactgccgat accaccatca gcgcctgcca gcaagccgga agcgaagatt tcgtcgtaac
    78421 cgttatagag caccagatca ggatgttcac gacggatctg ttccatctga tagagatcgc
    78481 cagaagtctg tttcagcgca ccgacgccag gcaacgtaac aagtgtgttg atctgatcca
    78541 gggtcagttt taccccactc agggctggaa tgttgtacac caccatcggc aacccatccg
    78601 ctgaatcaat aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga
    78661 acggcgtgac ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt
    78721 ggctttcggc ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct
    78781 cttcggcgac gatttccagt acctgttcac gctcggaaag gctttgtaca aaggcttcgc
    78841 cggtcgaacc acccacgtat aaaccgtcaa tgccctgctg aatattgaac tgaaccaggc
    78901 ggcgcagact cgctttatcc agtgcttgtt gttgatcaaa aggagtcagg agtgcagcca
    78961 ttacgccacg taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga
    79021 tataccttta tacctgttat accagatcaa ttaagcaaca ccccatacag aaagctgata
    79081 atgcgatctg cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaggcg
    79141 tgccaggtag cagagacgct gttgagatgc gattgcaacg cacgatcggc ttcgtcagga
    79201 tcatgacgcc ggatcgcgtc aacgatcgca atatgctgtt gatagctaac gttgttatgc
    79261 tcgtgcagag cctgatcagc cacggttgga cgtgcggcga tcagccagtc gagcagggca
    79321 acgtggatcg ccatgaagat tgggttacct gggatctccg ccagcacgcg gtggaaatca
    79381 acgtctgagc gaatgaatgc cgcgttgtta tccagcgact gactgttgat ttccagtgct
    79441 tttgccagca aatcgatttg ctcatcggtg gcgtgttcag ccgcatagcg caccaggctg
    79501 gattcaaaga acagacgtaa ttgttcgaaa tgggcaatcc caccaggatg agaaaggaaa
    79561 tctttcgcca tgccggaaag ctcaccgatg atggtgtccg cagaaggacg cgagacgcga
    79621 gcgcgttcgc cgttgtttat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttca
    79681 cgcaccgaag gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt
    79741 tcaccttcgc caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg
    79801 gagagttttt tacgcgccag cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa
    79861 tcttcggttt gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca gtgtgattaa
    79921 catcatcagt gacatcctat cacaggattg aaagtagggg aaaatggcag ggttttctct
    79981 ttgtgcctca tcattaccat aattaacgga ataattaact attgcgaaaa attaatgtaa
    80041 cgcagataaa aacatcccga ttgaattatt tacaagacta ttcacgagca ttatgaatat
    80101 tatgaatgtg ttcttacaaa ataatcataa gcgcatattt tttaatgaaa aatcacctca
    80161 ccta
//
LOCUS       DAZGBC010000004        77617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGBC010000004 DAZGBC010000000
VERSION     DAZGBC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.004"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..791
                     /gene="ynaI"
                     /locus_tag="ACHK1J_000254"
     CDS             <1..791
                     /gene="ynaI"
                     /locus_tag="ACHK1J_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO7909594.1"
                     /translation="CTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKIT
                     IIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGD
                     WIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTI
                     GLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVW
                     AEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
     gene            complement(842..2455)
                     /gene="mppA"
                     /locus_tag="ACHK1J_000255"
     CDS             complement(842..2455)
                     /gene="mppA"
                     /locus_tag="ACHK1J_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO7909595.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKSGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2792..3691)
                     /gene="pgrR"
                     /locus_tag="ACHK1J_000256"
     CDS             complement(2792..3691)
                     /gene="pgrR"
                     /locus_tag="ACHK1J_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO7909596.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSYPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLRGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3829..4749
                     /locus_tag="ACHK1J_000257"
     CDS             3829..4749
                     /locus_tag="ACHK1J_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7909597.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            4749..5678
                     /locus_tag="ACHK1J_000258"
     CDS             4749..5678
                     /locus_tag="ACHK1J_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7909598.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHL"
     gene            5699..6337
                     /locus_tag="ACHK1J_000259"
     CDS             5699..6337
                     /locus_tag="ACHK1J_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7909599.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            6628..7356
                     /gene="mpaA"
                     /locus_tag="ACHK1J_000260"
     CDS             6628..7356
                     /gene="mpaA"
                     /locus_tag="ACHK1J_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO7909600.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(7331..8296)
                     /gene="ycjG"
                     /locus_tag="ACHK1J_000261"
     CDS             complement(7331..8296)
                     /gene="ycjG"
                     /locus_tag="ACHK1J_000261"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO7909601.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQQILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFNLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEQALQFTTGELHL"
     gene            8415..8921
                     /gene="tpx"
                     /locus_tag="ACHK1J_000262"
     CDS             8415..8921
                     /gene="tpx"
                     /locus_tag="ACHK1J_000262"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO7909602.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(8965..10506)
                     /gene="tyrR"
                     /locus_tag="ACHK1J_000263"
     CDS             complement(8965..10506)
                     /gene="tyrR"
                     /locus_tag="ACHK1J_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO7909603.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(10654..11715)
                     /gene="ycjF"
                     /locus_tag="ACHK1J_000264"
     CDS             complement(10654..11715)
                     /gene="ycjF"
                     /locus_tag="ACHK1J_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO7909604.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQVQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(11712..13109)
                     /gene="ycjX"
                     /locus_tag="ACHK1J_000265"
     CDS             complement(11712..13109)
                     /gene="ycjX"
                     /locus_tag="ACHK1J_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO7909605.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13264..14262
                     /gene="ycjW"
                     /locus_tag="ACHK1J_000266"
     CDS             13264..14262
                     /gene="ycjW"
                     /locus_tag="ACHK1J_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7909606.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPHQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMSLK"
     gene            complement(14313..15218)
                     /gene="ompG"
                     /locus_tag="ACHK1J_000267"
     CDS             complement(14313..15218)
                     /gene="ompG"
                     /locus_tag="ACHK1J_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO7909607.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(15263..16345)
                     /locus_tag="ACHK1J_000268"
     CDS             complement(15263..16345)
                     /locus_tag="ACHK1J_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7909608.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(16359..17018)
                     /gene="pgmB"
                     /locus_tag="ACHK1J_000269"
     CDS             complement(16359..17018)
                     /gene="pgmB"
                     /locus_tag="ACHK1J_000269"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO7909609.1"
                     /translation="MKLQGVVFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGDKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLCAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDFLTWSRLSAFWQN
                     V"
     gene            complement(17015..19282)
                     /gene="ycjT"
                     /locus_tag="ACHK1J_000270"
     CDS             complement(17015..19282)
                     /gene="ycjT"
                     /locus_tag="ACHK1J_000270"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO7909610.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLCLLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGLSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHREDE"
     gene            complement(19279..20334)
                     /gene="ycjS"
                     /locus_tag="ACHK1J_000271"
     CDS             complement(19279..20334)
                     /gene="ycjS"
                     /locus_tag="ACHK1J_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7909611.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVNPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQARVMRDTARRQGKVLAYDFHHRFALDTQQLREQVTNGILGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(20344..21132)
                     /gene="ycjR"
                     /locus_tag="ACHK1J_000272"
     CDS             complement(20344..21132)
                     /gene="ycjR"
                     /locus_tag="ACHK1J_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7909612.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEVLAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(21150..22202)
                     /gene="ycjQ"
                     /locus_tag="ACHK1J_000273"
     CDS             complement(21150..22202)
                     /gene="ycjQ"
                     /locus_tag="ACHK1J_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO7909613.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPAGAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(22233..23075)
                     /gene="ycjP"
                     /locus_tag="ACHK1J_000274"
     CDS             complement(22233..23075)
                     /gene="ycjP"
                     /locus_tag="ACHK1J_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7909614.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(23062..23943)
                     /gene="ycjO"
                     /locus_tag="ACHK1J_000275"
     CDS             complement(23062..23943)
                     /gene="ycjO"
                     /locus_tag="ACHK1J_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7909615.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(23964..25256)
                     /gene="ycjN"
                     /locus_tag="ACHK1J_000276"
     CDS             complement(23964..25256)
                     /gene="ycjN"
                     /locus_tag="ACHK1J_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7909616.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            complement(25270..26949)
                     /gene="ycjM"
                     /locus_tag="ACHK1J_000277"
     CDS             complement(25270..26949)
                     /gene="ycjM"
                     /locus_tag="ACHK1J_000277"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO7909617.1"
                     /translation="MKQEITDYLAEIYGGTFTSTHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSGKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSEEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSN
                     AEGNCLTGLFNVSKNIQHVNITGLHGRDLISEVDILGNKITLRPWQVMWIK"
     gene            complement(27159..27473)
                     /gene="pspE"
                     /locus_tag="ACHK1J_000278"
     CDS             complement(27159..27473)
                     /gene="pspE"
                     /locus_tag="ACHK1J_000278"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO7909618.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(27548..27769)
                     /gene="pspD"
                     /locus_tag="ACHK1J_000279"
     CDS             complement(27548..27769)
                     /gene="pspD"
                     /locus_tag="ACHK1J_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO7909619.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(27778..28137)
                     /gene="pspC"
                     /locus_tag="ACHK1J_000280"
     CDS             complement(27778..28137)
                     /gene="pspC"
                     /locus_tag="ACHK1J_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO7909620.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(28137..28361)
                     /gene="pspB"
                     /locus_tag="ACHK1J_000281"
     CDS             complement(28137..28361)
                     /gene="pspB"
                     /locus_tag="ACHK1J_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO7909621.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(28415..29083)
                     /gene="pspA"
                     /locus_tag="ACHK1J_000282"
     CDS             complement(28415..29083)
                     /gene="pspA"
                     /locus_tag="ACHK1J_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO7909622.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            29250..30227
                     /gene="pspF"
                     /locus_tag="ACHK1J_000283"
     CDS             29250..30227
                     /gene="pspF"
                     /locus_tag="ACHK1J_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO7909623.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIILDPFKRRPPEEANAVSENTSLPMLP
                     LDLREFQMQQEKELLQHSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(30347..31612)
                     /gene="puuE"
                     /locus_tag="ACHK1J_000284"
     CDS             complement(30347..31612)
                     /gene="puuE"
                     /locus_tag="ACHK1J_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO7909624.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHDVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(31650..32930)
                     /gene="puuB"
                     /locus_tag="ACHK1J_000285"
     CDS             complement(31650..32930)
                     /gene="puuB"
                     /locus_tag="ACHK1J_000285"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO7909625.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTTKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(32932..34419)
                     /gene="puuC"
                     /locus_tag="ACHK1J_000286"
     CDS             complement(32932..34419)
                     /gene="puuC"
                     /locus_tag="ACHK1J_000286"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO7909626.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDLDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(34569..35126)
                     /gene="puuR"
                     /locus_tag="ACHK1J_000287"
     CDS             complement(34569..35126)
                     /gene="puuR"
                     /locus_tag="ACHK1J_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO7909627.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(35153..35917)
                     /gene="puuD"
                     /locus_tag="ACHK1J_000288"
     CDS             complement(35153..35917)
                     /gene="puuD"
                     /locus_tag="ACHK1J_000288"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO7909628.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            36129..37547
                     /gene="puuA"
                     /locus_tag="ACHK1J_000289"
     CDS             36129..37547
                     /gene="puuA"
                     /locus_tag="ACHK1J_000289"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO7909629.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQDGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            37696..37860
                     /locus_tag="ACHK1J_000290"
     CDS             37696..37860
                     /locus_tag="ACHK1J_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7909630.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            37850..39235
                     /gene="puuP"
                     /locus_tag="ACHK1J_000291"
     CDS             37850..39235
                     /gene="puuP"
                     /locus_tag="ACHK1J_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO7909631.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            39369..39614
                     /gene="ymjA"
                     /locus_tag="ACHK1J_000292"
     CDS             39369..39614
                     /gene="ymjA"
                     /locus_tag="ACHK1J_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO7909632.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            39927..41570
                     /gene="sapA"
                     /locus_tag="ACHK1J_000293"
     CDS             39927..41570
                     /gene="sapA"
                     /locus_tag="ACHK1J_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO7909633.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVSVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            41567..42532
                     /gene="sapB"
                     /locus_tag="ACHK1J_000294"
     CDS             41567..42532
                     /gene="sapB"
                     /locus_tag="ACHK1J_000294"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO7909634.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            42519..43409
                     /gene="sapC"
                     /locus_tag="ACHK1J_000295"
     CDS             42519..43409
                     /gene="sapC"
                     /locus_tag="ACHK1J_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO7909635.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            43409..44401
                     /gene="sapD"
                     /locus_tag="ACHK1J_000296"
     CDS             43409..44401
                     /gene="sapD"
                     /locus_tag="ACHK1J_000296"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO7909636.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            44403..45209
                     /gene="sapF"
                     /locus_tag="ACHK1J_000297"
     CDS             44403..45209
                     /gene="sapF"
                     /locus_tag="ACHK1J_000297"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO7909637.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(45260..46423)
                     /locus_tag="ACHK1J_000298"
     CDS             complement(45260..46423)
                     /locus_tag="ACHK1J_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO7909638.1"
                     /translation="MARVSPSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(46432..47805)
                     /locus_tag="ACHK1J_000299"
     CDS             complement(46432..47805)
                     /locus_tag="ACHK1J_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO7909639.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(47809..50916)
                     /locus_tag="ACHK1J_000300"
     CDS             complement(47809..50916)
                     /locus_tag="ACHK1J_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO7909640.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYLVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     SRRQE"
     gene            complement(50916..52037)
                     /locus_tag="ACHK1J_000301"
     CDS             complement(50916..52037)
                     /locus_tag="ACHK1J_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO7909641.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VTELTGRTSAAFSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(52186..52752)
                     /locus_tag="ACHK1J_000302"
     CDS             complement(52186..52752)
                     /locus_tag="ACHK1J_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7909642.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            53230..54018
                     /gene="fabI"
                     /locus_tag="ACHK1J_000303"
     CDS             53230..54018
                     /gene="fabI"
                     /locus_tag="ACHK1J_000303"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO7909643.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSNIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            54162..55289
                     /gene="yciW"
                     /locus_tag="ACHK1J_000304"
     CDS             54162..55289
                     /gene="yciW"
                     /locus_tag="ACHK1J_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO7909644.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISTLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            55357..57291
                     /gene="rnb"
                     /locus_tag="ACHK1J_000305"
     CDS             55357..57291
                     /gene="rnb"
                     /locus_tag="ACHK1J_000305"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO7909645.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(57410..57472)
                     /locus_tag="ACHK1J_000306"
                     /pseudo
     CDS             complement(57410..57472)
                     /locus_tag="ACHK1J_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            57526..59511
                     /gene="pdeR"
                     /locus_tag="ACHK1J_000307"
     CDS             57526..59511
                     /gene="pdeR"
                     /locus_tag="ACHK1J_000307"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7909646.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(59493..59621)
                     /gene="ymiD"
                     /locus_tag="ACHK1J_000308"
     CDS             complement(59493..59621)
                     /gene="ymiD"
                     /locus_tag="ACHK1J_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO7909647.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            59659..59832
                     /gene="yciZ"
                     /locus_tag="ACHK1J_000309"
     CDS             59659..59832
                     /gene="yciZ"
                     /locus_tag="ACHK1J_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO7909648.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            59922..60671
                     /gene="yciT"
                     /locus_tag="ACHK1J_000310"
     CDS             59922..60671
                     /gene="yciT"
                     /locus_tag="ACHK1J_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO7909649.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            60940..61158
                     /gene="osmB"
                     /locus_tag="ACHK1J_000311"
     CDS             60940..61158
                     /gene="osmB"
                     /locus_tag="ACHK1J_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO7909650.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(61284..61610)
                     /gene="yciH"
                     /locus_tag="ACHK1J_000312"
     CDS             complement(61284..61610)
                     /gene="yciH"
                     /locus_tag="ACHK1J_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO7909651.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAPPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDIDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(61610..62347)
                     /gene="pyrF"
                     /locus_tag="ACHK1J_000313"
     CDS             complement(61610..62347)
                     /gene="pyrF"
                     /locus_tag="ACHK1J_000313"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO7909652.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPYGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(62540..63709)
                     /gene="lapB"
                     /locus_tag="ACHK1J_000314"
     CDS             complement(62540..63709)
                     /gene="lapB"
                     /locus_tag="ACHK1J_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO7909653.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(63716..64024)
                     /gene="lapA"
                     /locus_tag="ACHK1J_000315"
     CDS             complement(63716..64024)
                     /gene="lapA"
                     /locus_tag="ACHK1J_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO7909654.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(64173..64937)
                     /gene="pgpB"
                     /locus_tag="ACHK1J_000316"
     CDS             complement(64173..64937)
                     /gene="pgpB"
                     /locus_tag="ACHK1J_000316"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO7909655.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            65107..65697
                     /gene="ribA"
                     /locus_tag="ACHK1J_000317"
     CDS             65107..65697
                     /gene="ribA"
                     /locus_tag="ACHK1J_000317"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO7909656.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(65761..68436)
                     /gene="acnA"
                     /locus_tag="ACHK1J_000318"
     CDS             complement(65761..68436)
                     /gene="acnA"
                     /locus_tag="ACHK1J_000318"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO7909657.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(68600..68695)
                     /gene="ymiC"
                     /locus_tag="ACHK1J_000319"
     CDS             complement(68600..68695)
                     /gene="ymiC"
                     /locus_tag="ACHK1J_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO7909658.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(68809..68976)
                     /gene="yciX"
                     /locus_tag="ACHK1J_000320"
     CDS             complement(68809..68976)
                     /gene="yciX"
                     /locus_tag="ACHK1J_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO7909659.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(68979..69119)
                     /gene="ymiA"
                     /locus_tag="ACHK1J_000321"
     CDS             complement(68979..69119)
                     /gene="ymiA"
                     /locus_tag="ACHK1J_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO7909660.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(69438..70412)
                     /gene="cysB"
                     /locus_tag="ACHK1J_000322"
     CDS             complement(69438..70412)
                     /gene="cysB"
                     /locus_tag="ACHK1J_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO7909661.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(70622..73219)
                     /gene="topA"
                     /locus_tag="ACHK1J_000323"
     CDS             complement(70622..73219)
                     /gene="topA"
                     /locus_tag="ACHK1J_000323"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO7909662.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKHQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            73599..73850
                     /gene="yciN"
                     /locus_tag="ACHK1J_000324"
     CDS             73599..73850
                     /gene="yciN"
                     /locus_tag="ACHK1J_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO7909663.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(73886..74935)
                     /gene="sohB"
                     /locus_tag="ACHK1J_000325"
     CDS             complement(73886..74935)
                     /gene="sohB"
                     /locus_tag="ACHK1J_000325"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO7909664.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            75155..75913
                     /gene="yciK"
                     /locus_tag="ACHK1J_000326"
     CDS             75155..75913
                     /gene="yciK"
                     /locus_tag="ACHK1J_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO7909665.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            75910..76500
                     /gene="cobO"
                     /locus_tag="ACHK1J_000327"
     CDS             75910..76500
                     /gene="cobO"
                     /locus_tag="ACHK1J_000327"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO7909666.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(76540..77415)
                     /gene="rluB"
                     /locus_tag="ACHK1J_000328"
     CDS             complement(76540..77415)
                     /gene="rluB"
                     /locus_tag="ACHK1J_000328"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO7909667.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
BASE COUNT        18896 a        20478 c        18966 g        19277 t
ORIGIN      
        1 tctgtacgtc cctgattgcg gttattctga cgattaagct atttttgctg attaatcagt
       61 ttgaaaaaca gcagattaaa aaaggtcgcg atatcaccag tgcgcggatt atgtcgcgca
      121 ttatcaaaat caccattatt gtggtgcttg ttctgcttta tggcgaacat ttcggcatga
      181 gcctttctgg cttgctgacc tttggtggta ttggtggtct ggctgtcggt atggccggta
      241 aagatattct gagtaatttc ttttccggga tcatgctcta tttcgaccgt cctttcagta
      301 ttggcgactg gatccgttca ccggacagaa atatcgaagg tacagtagcg gaaattggct
      361 ggcgaattac caaaattacg acctttgata atcgtccatt gtacgtaccg aactcgctgt
      421 tttcgtcgat cagcgtagaa aacccaggac gaatgaccaa ccgccgcatt accacgacca
      481 ttggtttacg ttatgaggat gcggcaaaag tgggcgttat cgtcgaagcc gtacgtgaga
      541 tgctgaaaaa tcacccggcc atcgaccagc gacaaacctt actggtttat ttcaaccagt
      601 ttgctgactc gtcattgaat attatggttt attgctttac caaaaccacg gtatgggctg
      661 agtggcttgc tgcacagcaa gacgtttatt tgaagattat cgatattgta cagtcacacg
      721 gcgcggactt tgccttcccc agccagacgc tgtatatgga taacattaca ccgccggaac
      781 agggtcgcta aatcagacat cactggcgtt atccacaagg gtaacgccag ttcccatcac
      841 atcaatgctt cacaatatac atagtccgac tgtacgccac atcttcaggg ttattaatgg
      901 gataaccttt cagccacggt ttgattaatc gtccattggt atattgataa attggtgcaa
      961 tcggtgcttg ctccatgagg attttttctg ccgcgttgta atcggcatta cgcgctttaa
     1021 cggtattttc cgtcgatgcc tgggccagaa ctttgtcgta tgccggattg ttaaagcgtg
     1081 aaatatttcc tgaatgcgtt gacgttaata atgtcaggaa agtggagggt tcattataat
     1141 cccccaccca tgaggcgcgg ataacatcaa aattgccggt gttacggcta tcgatatagg
     1201 ttttccattc ctgattttgc aatttaacat ctacgccaag gttctttttc cacatcgatg
     1261 ctacagcaat cgcaattttt tgatggtttt ctgaagtgtt atacaaaagc gtcagcttca
     1321 gcggtttttg cggaccataa ccagctgcgc tcaacaaagt ttttgcctgc gcattcagtt
     1381 cttcctgact catttgttca aacggcgaag gttccggcgt aaatccggcg gtaacatctg
     1441 gtgtaaaatg ccacgctggc ttttcgcccg tccctaatac tttttcggtc atcaggcggc
     1501 gatctatcgt catacttaat gccagacgaa cgcgctgatc tgccgtcgga cctttttgcg
     1561 tgttaaacgc ataataatag gtcccgagct gcggcggcgt ataaacctgc cccggaatat
     1621 ccttcaacag cttctgatac atatttttcg ggaaggattc ggtgatatca atatcccccg
     1681 cgaggtaacg cttagtggct gcggattcct gattaattgg caggaaggtc actttttgca
     1741 gtaccgtttt ggcgttatcc caataatggg tattcggtac cacgaccagt ttttcattga
     1801 ctacgcgttc tttaagaaca taagcgccat tgccgatcag atttccggat ttcgtccact
     1861 ctttaccgct ttctacgttg gctttttgca ccgggaagaa ggcaaagtta gcggttaaat
     1921 tcacaaacca cggcaacggt ttatcaagct gaattttcaa agtatgggca tcaactgcgg
     1981 tgacgccaag ctggtcaggc gtagctttac catcaataat cgcctgtgcg ttgttgattc
     2041 ccgccagcgc ggcaaaccat gcaaatggcg atagcgtttt cgggtccacc agacgctgcc
     2101 agctgtagac aaaatcttgc gccgttaccg gtgtgccatc cgcccatttt gcgttatcgc
     2161 gcagggtaaa agtccagata cggttgtcat tacttttcca ctgagtcgca acgccgggga
     2221 caatttcccc tttctcgttc tgattcacca gtccttcaaa cagatcgcga atgacctgaa
     2281 tctctggcag gcccacggct ttagcgggat ccagcgacgc aggctcatct ttaatgtggc
     2341 gcaccagctc ctgcttctct gccagtactg tgccgctcgg aacttctgca gcatacgaaa
     2401 gagaaatgct gctgaccaac agcgcacaac acgtgactga aacagagtgc ttcataagtt
     2461 tccctctaac cgacattaaa attgatgcgt aattatttgt ttcgccttaa agaaatgcaa
     2521 acaacttcca tcaaaaaagt gacacaggaa gcataaaatg ttttatgtat caatatatta
     2581 aatcagattt agtgtaaata atcgacgcca aaatgcggca aagcacatag aacaccgaac
     2641 actacgttcg ggcttcgcac gttcaaacca gttttcggta tcataatcag catctgtcgc
     2701 cattgtacga agaataaaaa ataaccaaaa cgttgaaagg aactgagaat acgttaagag
     2761 aagaattaaa ttaatgtatc agcaagtagt gctaaacagc acctttatac ttcagcctat
     2821 ctataaataa tgagaatgcc gagccagcat gtcgacggtg tggatagtac aggtgataac
     2881 cgcgtaaatc tggtgtgaat ttatccaaaa cacgtatcag ttttttttct ttaatagccc
     2941 gctcaacctg atcataaggt agatacgcca atccatgccc atcaattgca gcatcaatga
     3001 tcaggtctat cgtattcagt aaaagctgac cttccatgcg aacacgaact tcacgtccac
     3061 cgcgtattaa tctccagcga tttgctgtac ccgatgtggg aagatacaaa ttaattgcct
     3121 gatgatctat taattgtgac actgacgttg gaacacttcg gcgagaaaaa taatccggtg
     3181 agccaacaat agccattggt atatctggcc cgattcgaat ggcgatcata tctttatcca
     3241 tctccccacc cagacggacg cctgcatcaa aacgttcaga aacgacatcg gtcaaaccat
     3301 aatcaatggt gagctgaata tcaatttcag gatacgattt caggaatgtg cgcattgctg
     3361 gtaacaatat cgtttttgct gcatgttcta cagtagtaat acgtattgtc ccggatgggc
     3421 ggttctgcag atcgctcagg gatgtcaggg cagaatctat gtcatgcaac atagggccaa
     3481 gaacagacaa aagatgctcg cccgcttcag tgggtacaac gctgcgcgta gttcgcgtca
     3541 gaagccgcaa tcccaatcgt tcttctatac ggcgcactat ctggcttaaa gctgactgcg
     3601 ccatgctcag gcgggctgct gcgcgagtga agctacgctc ctctgcaacg acgacaaacg
     3661 ccatcagatc agcgatttct tcacgcttca ttcagtatct ccatacaatc tattaatcat
     3721 ttaaatgata ggtctaatca tgattttggg tctaatcaac ttaatgatca atatctatag
     3781 tgatatcacc aaccgttaaa acgagccaac aactccatga ggaacggaat gatgaataat
     3841 aaagtcagct tcactaacag caataatcca accatctctt tgtctgcagt gatctatttt
     3901 cctccaaaat ttgatgaaac ccgtcaatat ccggcaattg tggtatctca cccaggtggg
     3961 ggcgttaaag aacaaacggc cgggacctat gccgaaaaac tggcggaaaa gggatttgtc
     4021 actattgctt atgacgcatc ttatcagggt gaaagtggcg gcgagccgcg tcagttagaa
     4081 aacccataca ttcgtactga agacatcagt gccgtgattg attacctgac aacgctttct
     4141 tatgtcgaca atacccgaat tggtgcgatg ggaatttgtg ccggtgcagg atatacagcc
     4201 aatgctgcaa ttcaggatcg ccgcatcaag gccatcggta ccgttagtgc ggtcaatatt
     4261 ggttcgatgt tccgtaatgg ttgggaaaac aatgtgaaat ctattgatgc cctgccatac
     4321 gttgaagcgg gttcaaacgc cagaaccagc gatattagca gcggagaata cgccattatg
     4381 ccactggcac caatgaagga gtccgatgcg ccgaacgaag aactgcgtca ggcctgggag
     4441 tattaccaca cccctcgcgc gcagtatcca acagcaccgg gttacgctac tttgcgcagc
     4501 cttaaccaga ttattaccta tgatgcttac catatggcgg aagtgtacct gactcagccg
     4561 acgcagattg tggcgggcag ccaggcaggg agcaaatgga tgagtgacga cctgtacgat
     4621 cgggcatcaa gccaggataa acgctatcac attgttgaag gcgcaaacca tatggatctt
     4681 tatgacggca aagcctatgt ggctgaagca atttcagtat tagctccgtt ctttgaggaa
     4741 actctgtaat gaaacatatt caaatccgca atagtgatat ggcctggcat attgctgcca
     4801 atattcagtt tccaccaaat tttgatgagt caaaacagta tccggcgatt atcagcgtcc
     4861 atccttttgg cagttgtaag gagcagacgt ctggcaatat ctatggcaag gcattggctg
     4921 aaaaaggcta tgtcgttctc gcttatgacg ccagtttcca gggagaatct ggtggggagc
     4981 ctcgttggat agaagatccg acacagcgcg tagaggatat tagccgtgtt atcgattatg
     5041 cagtaactct gccatatgtt gatgctgaac gcattggcgt gctgggtgtg tgtggggggg
     5101 gtggctatgc cattaatgcc gcgctgacag aaaaacgaat taaagctgta gtgagtatta
     5161 cgggtgttaa tatcgggcgt ctttttcggg aaggtttcag caattacgat ccgattggcg
     5221 cattgaatgc aatggcgtcg caacgaacca aagaggctcg tggtggagaa ttacagatca
     5281 acgaattact tcccgccagc ctggatgcag caaaagccca tggactgacc gaacgcgatg
     5341 tttatgaagc aactgactac tataaaacac cacgcgggca acaaccggga ggtgcgacaa
     5401 aaatgttgtt ctcacatgca cagaaaacgc tggcctggga tgccttcgca tttactgaag
     5461 tgttactgac tcagccggtg atggtagtag ttggtgaaaa agtcggtgcc ttcggtgctt
     5521 atcgtgatgg actggaagtc tatggccggg cgatggtttc gcaggatcgt cagttagttt
     5581 cattacccga tttctcacat tatgaactgt atgacaaacc ggaagcagtt caggaggcgc
     5641 tggaaaaagt tattcctttt ttcaacaccc atctgtgata agtttcatga gaaatattat
     5701 gaaaaatgta ctcattcttg gtgctggcgg tcagatagcc cgccatgtga ttaatcaact
     5761 ggcagataaa caaaccatta aacaaacttt atttgctcgt cagccagcaa aaatacacaa
     5821 accctatcca actaatagcc agattattat gggggatgta ctgaatcatg cagcactgaa
     5881 gcaggccatg cagggacagg atattgtgta tgccaacctt acgggggaag atttagatat
     5941 tcaggcaaat tcagtgatcg ctgcgatgaa agcctgtgat gttaaacgtc tgatctttgt
     6001 tctttccctc ggcatttatg atgaagttcc aggaaaattt ggcgaatgga ataatgcagt
     6061 tattggtgag cccctaaaac cattccgcag ggctgcggat gcaattgagg cttctggact
     6121 ggaatataca attcttcgcc ctgcctggct gacggatgaa gatattattg attatgagct
     6181 tacctcacgc aatgaaccat tcaaaggtac gatcgtatca cgtaagagtg tcgcagcatt
     6241 aattacagat atcatcgata aacctgagaa acacattggt gagaatatag gcatcaatca
     6301 acccgggact gatggcgaca agccattctt catgtaaatc aattatatag ccaccatcgt
     6361 actgtactat gtgcagtacg atttattaaa cttattaagg tgaactgatc aatcagatgc
     6421 gattaacatg tgtagaacca gtaagaataa aatacgccat gtatataaca ttacgacaat
     6481 tctaatatct gcacatcaat gaaaatacaa acaacttccg gcaaaaaagt gatacaagat
     6541 cgatgacata gcgttgaaga aaaggatttg ataatccaca aaatttgcac aacactgcct
     6601 gtaatacttc ctgaaccaga gacgatcatg accgtaaccc gcccacgcgc cgaacgcggc
     6661 gcatttccgc ccggaacaga acattacgga cgttcattat tgggcgcgcc gttaatctgg
     6721 tttccggccc ctgcagccag tcgcgaaagt ggtttgattc tggctggcac tcacggtgat
     6781 gaaaactctt cggtcgtcac gctctcctgt gctctgcgga ctttgacacc ttctttgcgc
     6841 cgccatcatg tggtgctgtg tgtgaatcct gacggctgcc agctggggtt acgggccaat
     6901 gctaatggtg tggatttaaa cagaaacttt ccggcggcga actggaagga aggtgaaacg
     6961 gtttatcgct ggaacagcgc cgctgaagaa cgtgatgtcg tattactgac aggtgacaaa
     7021 cctggctccg aacctgaaac ccaggcgctg tgccagctta tacaccgcat tcaacctgcc
     7081 tgggtggtct cattccacga tccactggct tgtattgaag atcccagaca cagcgaatta
     7141 ggcgaatggc tggcccaggc gtttgaattg cctctggtaa ccagcgtcgg ttatgaaacg
     7201 cctggctctt tcggcagttg gtgtgcagat ctcaacttgc attgcatcac cgcagagttt
     7261 ccgcccatct cctccgacga agccagcgaa aaatatctct ttgcgatggc aaacttgctg
     7321 cgctggcatc ctaaagatgc aattcgcccg tcgtgaactg aagcgcctgt tccacatcta
     7381 ccgccagcca ggtcggtccg tcaagatcgg cgaaactgac ctgcggcacc agcggtaaag
     7441 cggcgctaat ggcacgagag gtacacaaca tgcagcccag catcagattg aaaccttgtg
     7501 cacgcgcttc agtcgccagc gccagcgctt ccgtcagccc cccggtttta tcgagcttaa
     7561 tgttaaccat ctcatagcgc cctttcagcg ccttcagatt gctacgagta tgacaacttt
     7621 catcagcaca aatcggcaac ggatgaataa aattctccag tgccgcatcg tcctgcgcag
     7681 gaagcggttg ttcaagcatc gcaacgccta aatccgccaa tagctggcaa cgtgccgcca
     7741 acccttctgc acgccaggat tcatttgcat caacgatcag cgtggcatcg ggcacagctg
     7801 tgcgaattgc caccatccgc tcactgataa gatggttatc cagcttcact ttcagtaatt
     7861 tcgcgccggc ctgccagagt gttgatgcac tattggccat ctgatcaggc gtaccgatga
     7921 ctaccgtctg tgcagtgata actgtctcgg gaagcgttat gccgatcaaa tcagccagcg
     7981 attgctgctg ttttcgcgcg gccagatccc acaatgcaca atccagcgca ttacgtgctg
     8041 cgccggcagg gagaatttgt tgcaactcct cccgtgtcag ccctttctct aattgcggca
     8101 cgacgctcat aatttgcgcc attaccgagg catcactttc tccataacgc ggatacggcg
     8161 tgcattcgcc ggtgccttta ataccctctt cttccagttc aaccaccacc acgcgcgctt
     8221 cactgcgact tccccgggca atcacaaacg gggtatgtaa gggccaggct tcctcgaata
     8281 ccttaacggt tctcatcttt actccttgtt gtcgcggtgg tctgcggatt aagcgcaaat
     8341 agcgtttgct gtgctattga cagttagcat aaactaggtg tgacgttaac tatatgtaaa
     8401 caggaaagat aatcatgtca caaaccgttc atttccaggg caacccggtt acagtcgcca
     8461 actccatccc gcaggcgggt agcaaagcgc agacttttac tctcgtggca aaagatctgt
     8521 ctgacgtcac cctcggtcag tttgcgggta aacgcaaagt gctgaacatt ttcccgagta
     8581 ttgataccgg tgtttgcgcc gcatcagtac gtaagtttaa ccaactggca accgagatcg
     8641 acaacaccgt tgtgctgtgt atttctgccg atctgccgtt cgcccagtct cgtttctgcg
     8701 gcgcagaagg tctgaacaac gttatcaccc tctccacttt ccgtaacgct gaatttctgc
     8761 aagcctacgg tgtggcaatt gctgatggcc cactgaaagg tctggcagcg cgtgccgttg
     8821 tggttattga tgaaaatgac aatgtgattt tcagtcagct ggtggatgaa atcaccaccg
     8881 agccggatta cgaagcagct ctggctgtac tgaaagcata agttaatatg cctgatggtg
     8941 ttgcaccatc aggcatattc gcgcttactc ttcgttcttc ttctgactca gaccatattc
     9001 ccgcaactta ttggcaatcg cggtatgtga aacgccgaga cgttttgcca gtttgcgcgt
     9061 gctgggataa ttgcgataaa gctgggttaa taccgagcgt tcaaaacggc tggtgatttc
     9121 atccagcgaa ccttccatcg catcttcgcc cacggctacc gttgcggcgt cataatccgg
     9181 caacaaaata tcctgtggac gcagttcata accgtccagt tgtgtcagtg cgcgatagat
     9241 agcgttcttt aactgccgca catttcccgg ccacgcataa cgcgtaagta cagtattcag
     9301 gtcagcggcc agtttcggac gcggcacgcc ctgctcgtcg gcaaagcggg cgacgaacag
     9361 ctcggttaac ggcatgatgt cctgcgggca gtcgcgtagc ggcggcagat tgagcgtcaa
     9421 cacgttcaga cgataataga gatcttcacg gaacacgcct ttttgcacca gttcgaccag
     9481 attcttctgc gtagcgcaaa tcacccgcac atcgacatgc acctcatggt cttcgccaac
     9541 ccgacggaaa gtgccatcat taaggaaacg cagtaatttc gcctgcatcc gtggtgacat
     9601 ctcacctatt tcatccaaca gcaccgaacc accgttcgcc tgctcaaaga atcctttctt
     9661 cccttccgga gcatgaccaa acagttcact ctcgaccgca tcttccggta tagacgcaca
     9721 gttcagcgcc aggtaaggtt tgctcgctct tgggcttgcc tgatggcagg cgtaggcaaa
     9781 aagatcttta cctgtacctg tgtcacccgt aatcagcagc ggcgcgctta gcatcgccag
     9841 tttctgcgcc tgttcgacaa catgcttcat tttcgggctg acagcgacaa tttgactgaa
     9901 ggcgctgacg tcctgggcgg cgacattttg caactgacgg cccatacgaa tcgttgatcg
     9961 caacatcacc accgcacctg tcaggacgtg ttgatcattt tcatcctgaa gataaacagg
    10021 cgtaatctcc atcaggaagt tctgtccatt aataacaaca tgctcgttat gggaatcttg
    10081 cggttcgctt tccagccaac gtaaaaaatt aaagccgtta atcaattgtg cggcggtatg
    10141 gttgcgcagg cgatccaatt tttgcccaaa aagctgacag ctcgccgggt tcgccatatc
    10201 cactttgctt ttcatatcga cagagagcac aggttcaggc aacgcctcca gtaacgcgct
    10261 caacgccaga tgctcacgtt cggaaggcat ccacgggaca gtacgcacat cggtaacacc
    10321 tgcaatacgg cgtatttcgg ccatcagact gctgaaactc tcaaactcca gttcagcaaa
    10381 attgaggtag attcgcccaa tgggatcaat ctcaataccg cgtaaatcaa tgcctcttag
    10441 cacgagtaga tcgagtaatt cgcgggtcag accgagtcgg tcttcacaaa agacttccag
    10501 acgcatggga accttcacct gaaaaaagaa caattaatat gatgatatga cactatttgg
    10561 atagcaggaa ggtttctgtc aacaatcatt gacacaaaga cggaaaaacg ggcacttcgg
    10621 cgtaaagata tccggtcagc acccgatatt gcattatttt tcgctgggcg ttttgccttt
    10681 ttgcagcgtt tctttcacct gaccgataag ctgacgacgg aaatccccga ggcgaggttt
    10741 gtcatcgtca atccacggca gcgggcggca aagctccata gctttaatcc cgagtcgtgc
    10801 cgtcagaagc ccagcaccaa tcccctgagc tgcgcgggta gacaaacgag cagcgagatc
    10861 ttgcgacatc cagtccatcc ccacttcgcg caccaattcg ctggctccgg cgaaagcgat
    10921 attcagcaat accagcttga acaggcgcaa acggctgtaa tatcccagtt caatgccata
    10981 cagcgtggcg atgcgattaa tcaaacgcag attgcgccag gcgataaatg ccatatccac
    11041 cagcgccagc gggctgaccg caatcatcaa cgttgattcc gctgccgaac ggctgatttc
    11101 gcgccgcgcc tgggcatcta aaactggctg gaccaaatgc gcatacaggc tgaccacttc
    11161 acggtcgttt tgcgtttcat ggattgaggc ataccagcgt tgcagcgccg gatgcgactg
    11221 atcaataccc gcctgctgcg ccagtttttc gcaaaatgcg cggcctttgc cggttccgtg
    11281 gctatgcaat aaatcacgcg cttcgtcgcg ttcatgggcg cgctgtcgca agcgccataa
    11341 gcgccgccac tctgttacca cagaacctac gccagcgccg ataatcaatg ccccagcggc
    11401 acatccaccc agcgccaccc agtcctgagt ttgccaggca ttcattgtcc actgtatacc
    11461 ctgcccgaca acgcttgcgc caaacagagc cagcccgccc atcaccattt tgcgccacag
    11521 gctgcgtttc ggacgtaacg ctgcgtccat taccgcttcg acttgcccct cttcttcctg
    11581 cgcttcgtcg agcgtggccg gggcaaaatt ttgcacctga ttttcgtcaa aggtctgctg
    11641 cgccctgaat ttaggattct gatcgacctc cagaggaccg tcgaaatcaa tacgtggttt
    11701 taacggttcg gtcatcgcaa tttatctcct attaaaaatt ccagcgcggc atcaagacga
    11761 atatgcggca gcggtttgtc gacatccatc acctgcggac gaaacgcctc aaactggaag
    11821 ccttgcttat cccagaacgc ctgaccaggc aaacgtgcgg ggacttcgcc aggataaaca
    11881 gtgagcggtg cgccatcact aagtcgatta ccgcgcagcg ccgggatttt ctcaccgtta
    11941 acatcaataa tgccgctggt ggtcgcctga acagacgcca gccccaggca gtccatgctg
    12001 atcccttcga acgccgcatt ttgccaggca tcctgaatca gttgttgcag taatgaaacc
    12061 atattagcgt gctgatcgat ggtcacatgg tccgctttag tggcagcaaa caatagctta
    12121 tcgataaccg gcgaaaacaa acgccggaac agggtacgct gcccgtagtg aaaactttgc
    12181 atcagttgcg tcagcgccag acgcatatca ttaaatgcct gtggcccact gttgagaggt
    12241 tgcaggcagt ccaccagcac aatctggcgg tcaaagcgca gaaaatgatt cttatagaac
    12301 cccttcacca ccttctcgca gtaataatta aaccgctcgc gcagcattcc ggcattggtg
    12361 tgcttatcgg cctgcgccag tttggactcg ccccaggcat cgacatccgg ccacgggaag
    12421 aattgcagcg cgggcgcacc tgccatatct cccggcaaga caaagcgccc tggctgaata
    12481 aagtgcagcc cctgctgttt acagtggtgg agataatcgg tccacgcggc ggcaatgtct
    12541 gccagccggt tttcgtcggc aggcgctagc gggtccagcc cttcgcacat cattcgccat
    12601 ttcgccgacc attcgccgcg ctgaccattg agtaagcccg tcatctggcg cgaccagctt
    12661 aaatagtcct gcgccagcat cggcaggtcg agcaaccatt cgccggggta atccacaatc
    12721 tccagataca gcgtggaggt atccttaaag tggcgcagca gcgaatcgtt cgatttatag
    12781 cgtagcgcca ggcgaatttc gctgacgccg cgcgttggcg tcggccaggc gggaggatcg
    12841 ccatacagct gcgccaaccc ttcgtcgtag gtaaaacgcg gaatgccaaa gtcacgctga
    12901 ggaatgcgtt ttacgcccag caggcgctct tcacgcaccg cacttaacag cggcaaacgt
    12961 gctccggcat gaatattgag caactgatta accatcgcgg tgataaacgc tgttttgccg
    13021 ctgcggctaa gtccggttac agcgaggcgc agatgtctgt cgacaccccg attcaccagc
    13081 gcattaagtt catttttaag tcgcttcatt gctgtcctgt gctgctctga agaccagaaa
    13141 aaaatcattc ataaggatat gaatgtgggg gccaggccat tgattatcaa ctacccgtgt
    13201 tgtcatcgac gggcgaactc cataagatgt gcagcattca cgttcggacg gagttcctga
    13261 cctatgtccc ctactattta tgatattgcc agggttgcag gcgtatcaaa atccaccgta
    13321 tcacgcgtgc tgaataagca aaccaatatc tccccggaag cgcgcgaaaa agtgttacgg
    13381 gccattgaag aattacagta tcaaccaaac aagctggccc gcgcgctgac ctcttcgggt
    13441 tttgatgcca ttatggtgat ttctacccgt tcgaccaaaa ctacggcggg taatccgttt
    13501 ttctctgaag ttttgcatgc catcaccgcc aaagctgaag aagaaggttt cgacgtgata
    13561 ttgcagacgt cgcacaaccc ggcagaagat ctgcaaaaat gtgaaagtaa aattaagcag
    13621 aaaatgatta aaggcattat tatgctaagc tcacctgctg atgaatcgtt ttttgcgcaa
    13681 ctcgataaat acgatattcc ggtggtggtg attggcaaag ttgaaggtca atatgcccat
    13741 gtttattctg tcgataccga taattttggc gacagcattg cgttgaccga tgcgttaatt
    13801 gaaagtggac atcaaaatat tgcctgtctg catgcaccgc ttgatgtcca tgtttcagtg
    13861 gatcgggtaa atggttataa gcagagtctg gaagatcata atattgcagt gcgtgatgaa
    13921 tggattgttg atggcggtta tacccatgaa acagcactgc aagcagcacg gcaattatta
    13981 agtcagtcac cgttgcctga ggcagtgttt gccactgaca gcctgaaatt aatgagcatt
    14041 tatcgtgcag cggcagagaa aaacattgct attccgcatc agttagcggt ggtgggttat
    14101 agtaatgaaa cgctgtcgtt tatattaacg cctgcaccgg gcggcatcga tgttccgacg
    14161 caggagttag gtcaacaaag ctgcgagtta ttattccgct taatttctgg aaaaccgtca
    14221 ccacagaata ttaccgttgc cacgcatatg tcgttgaaat aaatgtatgc cggatttggc
    14281 gctcgcgccg catccggcaa tttagcccat tatcagaacg agtaatttac gccgacacct
    14341 gcataatgga atttatcact gtctccttcg tcgtgatcct gccactcaaa cgcgtattcc
    14401 agcgaaacgg aaagaccatt ctggaaatca taaccgtaaa ataaaccaac acggttaaaa
    14461 tcatggcctt cacgttcaat atcatcctgc cagtcccagt tactccagcg atccagccca
    14521 atgcgcgtat acggcgtcac cgagtggttg ccgagcgtca gcggcaaata ggcgcgaatt
    14581 tcttgcgtgg aaaactcacc gttattgcgg ctgtcgtcca tattgaagcc gcgctcgaga
    14641 taatagttca ctcgcaaggc aaccgtttcg ttgaaggtat attgcagacc tgtttccgtt
    14701 tcgacacggg tatcagcgta accggtcgtg ttcagatcgt tggcaaattt atacatcgac
    14761 aaccaaccgt tgaaacgtaa atcgtcagtc agtttcacat cccagtctgg cgcgattttc
    14821 cagcgctgca tattcgccgt atctttaccc ggttcatcaa cgtagtgata accataatta
    14881 cggaaaccgc cggtcaggcc gaaactgaaa tcatcgcttt cgaggaactg ataatgcacc
    14941 tccagctccg ggcgatcaaa ccacgttcca cgtttacccg cgctataatc taccggccct
    15001 tcctgataat aggccagagc aattctccac ggcccgttgg cggcattaaa atagactgaa
    15061 ggctccgcca gcccatccat atcttcgcca taaccctcga cgttttctat ttcgtacatc
    15121 gcgccgatat taaagtgcca gtcgttcttt tcctcggcct gtgcgcaggc cattcccgca
    15181 cacatcacca gtgcggtaca gggtaataac tttttcatta ttgttccttg tattattctg
    15241 ggggaatgcc ttccccctgt atttagcgaa ttgctatttc cgtttctgca tcaaagaaat
    15301 gacatttcgt catatcaaaa tgaatagtga tattttctcc tgcatgataa tcatttaacg
    15361 caccagcacg gaccactaac tcgtgccccc caaccgtggt gtagagcata aattccgcac
    15421 cggttaattc tgccacgcta attttggcgg aaatgttatt ttcctcttgc gcgtccggat
    15481 gaatatcttc cggacgtatt cccatcacga tgggcttatg caaacttccc tgtgttttca
    15541 gaaccgctaa tttctcttcg ggaatggtta atttaagcgt ttccgtaacg aatttatcgc
    15601 catcgatcgt gccacgaata aaattcatcg ccggtgatcc aataaatccg gcaacaaaca
    15661 tattcgctgg ttggttataa accgttttcg gcgcaccaac ttgctgaaca atcccatcct
    15721 tcataatcac aatccgcgtc gccatggtca tcgcttcggt ctgatcgtgg gtcacgtaga
    15781 tcatggtggt attcagcttc tggtgcagct tgctgatctc tgcgcgcatt tgcacgcgca
    15841 gcttggcatc aaggttagag agcggttcat ccattaaaaa cacgcccgct tcgcgtacaa
    15901 tcgcccgccc gagcgccaca cgctggcgtt gcccgccgga aagcgcgccc ggcttgcgtt
    15961 tcaggtactc acgcaggccg agaatttgcg cagcccagtt aacgcgctca tcaatcacct
    16021 ctttggcgat tttttgcatc ttcaggccaa acgccatgtt gtcgtagacc gtcatatgcg
    16081 gatacagcgc atagttctgg aacaccatcg cgatattgcg ctctttagcc ggaacgtcat
    16141 tcattcgttt gccgtcgatc aacagatcgc cgccgctgat ctcctcaagc ccggcaatca
    16201 tgcgcagcgt agtcgattta ccgcagcctg acggaccgac aaatacgatg aactctttat
    16261 cggcaatttc caggttgaag tccttcacca catgcacctg gttatcgtag attttttgaa
    16321 tatgttgtaa cgaaagctga gccatgttga ttcctttgct atacgttttg ccagaaggcc
    16381 gataaccgcg accaggtcag gaagtccgtt gagggcaaca gtaattgcgc cccggttaag
    16441 cccgcgccga tccccaccga gcgcatacca ctggcgttaa tggcgtcaat gcccgcctgc
    16501 gcatcttcaa tgccgataca tgcctgcggc ggcacgccca gcccggcaca ggcagcgaga
    16561 aagatttccg ggtccggttt cgagttttta agttgggaag cgtccgcgca gaaggtgaaa
    16621 aactcacgta gctccaacgc cgctaaaatc gtcggcgcat tcagggagac agaagccagc
    16681 cctataggga tctgctgtgc gcagagatct gccagcaaag agcgaatgcc gggcaacaca
    16741 gcattaaccg tcagttcgcg cagtgagtgg acgtagagca gatttttgcg atacgccagt
    16801 tgcgcccgct cctgcgggtt aaagtcgccc tctttgtccc cgtgttgcag aatgcgccgc
    16861 agagactcat cgcggctgat cccttttagg gattcgttaa actgcgcatc aatgctgatg
    16921 ccaatttcag cagcaatctg ctgccacgcc ttgaaatgca gatgcgcggt atcggtgatt
    16981 acaccatcca ggtcgaaaac tactccttgc agtttcattc atcctcccga tgcgtggtag
    17041 cggtcccatt gaagggcaaa ataaaatcgc ccaaacagaa aacagattct tcagccacgg
    17101 atataagctg gccgttcaaa cgcagtgaaa cgggcgcaga agttcgaatc gcaatacgct
    17161 gcgcgtcgag cgtgacctgt aattcgcagc cctgccagaa cagagggaaa gacaactgtt
    17221 gccactgctc aggtaacgcc ggattgagat gcaattcacc gtcacgcacg ctcaacccgg
    17281 caaaaccctg aatcgccccc agccagatag cgccagttgc ggcagcgtgg ataccatcat
    17341 cacaactatg cggatcagca ccaagatcga tttcagtccc ctcgcgccag aactgataac
    17401 tttgggtcag caggccacag cgtgcggcaa caatgccgtg gattgcttta cttaatgacg
    17461 agtcgtgaat agtgcgcggt tcataaaatt gcagattggc aagacacgat gccgctgaga
    17521 actgctccgg cagcatgtaa ttgagcatca ccacatcagc ttgtttgagg atctgcatct
    17581 cgttcacttc tgcgcgtgaa taatccagca gaatggtttg ctttcccgcc gccgctttgt
    17641 atttcgccag gttaatcgcc ggcttagcca taaacgaatc atcctgcggc aaaacgccgt
    17701 cgggctgaat ttctggcagc cgcagttctt taaggaacat ttcggcgcga tggataaacg
    17761 catcgtcgct acagccaaat tgacgagcga tgttcagcgc ctgctggacg ttgtaatacg
    17821 ccatatagct ggtgaaggcg ttattattga catgttcggt atattcgtct ggcccaataa
    17881 catcatgaat ttccagacga tcgttaactc tcaccgcgcg gctaatccag aactttgccg
    17941 tctccagaag tagcgccatg ccttcatgag caatgaaact ttcatcccct gtggtctgcc
    18001 agtattgaat aaccgcccag gcgatatcgg cgaccagatg atgttccgcc tgcgccgaag
    18061 ccactttttg ccgcagcccg gtgcgaatgt taatggcggc aaattccggc gtctcttctt
    18121 cgccgctgcg cgcgctttcc cacggaaata gcgcgccctg ccagccgttg cgtcgcgctt
    18181 tctcctgcgc gcctggcaag ttgtgccagc gataacgcag taaacttcgg gcaaccgtcg
    18241 gatcgctaaa cagatgaaac ggtagcaaaa atacttctgt atcccagaaa acgtggcctt
    18301 tgtagccttc tccggttaag ccttttgccg caatactgct gcgctcgtcg tgagcaggcg
    18361 tcatgatgcg cagatgataa agcgcataat ctaacgcttg ctgatcgtgc gcatcgccgc
    18421 cgtttaccgt gatacgacgt ttctgccacc attgacgcca gttttctgtt gatgctgcaa
    18481 gaagttggtc gtaactctgc tgcgcgcaca tttcaagcag gcaaagcgac gcgctgcccc
    18541 actcgtctaa aacggcttgc ctgtcatccc gccagtcgat ccacaccagt ttttgcaacg
    18601 tcactgtctc gcctgcatga agctgcgcac tggtatgttg cagcaaacgg cgctctttag
    18661 ccgtatagca ttgctgaacg tcaccactca cctggcagca acagctaatt gccacatcac
    18721 tgcgtccatc ctgagtggta tagattcctt gcatcaaatg ctgtccaaac actcgcacct
    18781 gggtttcgtc gagatgctgg cgaccgtggt tggtttgcgt ggcgtcaatg cctgttgaaa
    18841 tcagcactga cgcgtcggcg tccagtggag taatagtgat ttccagcgca atgagcggca
    18901 gttggtctgc cgaaacaaag cgacggctgg cgatggtgta acctgtgccg ttgctggtac
    18961 gccagacaac gctgcggcgt aattcgccac tggcaaagtc aagctcacgc tgccaggctt
    19021 cgcgggataa tgagaaaatc tcaccattta tggcaatctc catccccacc acatcgggca
    19081 ggttcaccag ttcgttgatt tcacctttac ccgcccgatg atacagcccc gccagataca
    19141 tgccgcgcgt ctggcgcgtg taatcttctt catggctggc gcgaagccca agatagccgt
    19201 tcccctgcgc catcagtgat gcatacttgt tcagggtatg ctggctgaaa tggggttctg
    19261 ataacgttac tggcctggtc ataattccac acgcgtccct gtttctgcgg attgatacaa
    19321 cgccgccacc agttgctgga tgatgtaccc ctgctcggca tcggctatca tcacgggctt
    19381 gccctgtacg tgattgataa aggcttccat gctgcgcaaa tggcggttgt cgtctgccat
    19441 ttcccgttgc atcagcgtca ttaactcacc gttgttatcg gtgtagatat gtgctggaaa
    19501 cagcgtcgca ccggctttat caccacaaaa gctgacgttc ataatcgact gttcgcggat
    19561 gttgagcgca aatgacgttt ccagccacag aatgccgccg ttatgaaatt caatggtgcc
    19621 aaacagcgaa tcttcgacgc tgtaagttgc cggatcccac tcgccaaatt gaccacagct
    19681 cttttgcgtg ccgatctttt gaaagctatg cgcattcacg cttttcaccg ccggaaagcc
    19741 cagcacatac atcgccgcat ccagcatatg aataccgatg tcgatcagcg ggccaccgcc
    19801 ctgcagttct ttattggtaa agacacccca gccgggaacg ccgcagcgac gcagggcgcg
    19861 ggcggtggtg acgtaaattt cgcccaggat gccgttagtc acttgctcac gcagttgttg
    19921 tgtatcaagc gcaaaacggt gatggaagtc gtaggccagt acttttcctt gtctgcgcgc
    19981 ggtatcgcgc atgacccgag cctgttccgg cgtcatagca ggcggctttt cgcacatcac
    20041 atggcagccg gcttccagtg ccataagggt atgttcatag tgaaaacggt taggtgagca
    20101 gacgctaacc acgtcagggt tcaccgccag cagcatggcc tgcggatcgt cccacacgga
    20161 tgcattaccg tatttatctg ccagcgcctg cgcctgggaa aggcggctgt cacagacagc
    20221 caccagttcc agatcgttgc gggtgcagta gtacgaagca tgaactttat ccgccacctg
    20281 gcccgcgcct atgatcgcga cccgcagcgg agagcttgtc attgcacttt tcacaaagac
    20341 ctcttagcag gtacgcaacc aggccaaaga atcacggtac gcctgggcag gatcttctgc
    20401 ccgaatgcgc ccttcataca ccacatagcc ctgataatta tcggcgcgca gttgttcaaa
    20461 cagcgcgtgg aaatccaggg tgccgctgcc cggctgataa cgatggttat cggcaatatg
    20521 cacatgaccg agcaggtcgc ggttgtcatg cagcgcctgc gccaggttat cttcttcaat
    20581 attcatgtga tagaaatcgc cgataatctg cacatgttta agatcgtttt cgacgatgta
    20641 acggcgggca tcggcgagag tgttgatcat gtgatcctga taacggttta acggttcgag
    20701 atacaccacg gttccggtac gcgcggcgac ctgttccagt acgcgcaggg aatcactcac
    20761 cattttacga tcgccgtcca ggctgcgcgg cgaggtcatc ggcggtaagc ggaaggtaaa
    20821 catgccccac gctgccggaa cgacgatacc tttaccgccc acttctgcca gcacttcgag
    20881 aatgcgctct atctgcttta agccattaag acgacgctct tcgataaagt caccaatcca
    20941 gccgtcatag ccaccgcagg cggtggtcac cggtaaaccg gtttctttga ttgccgcttt
    21001 gacttcttcg atgttgttga ccagcagttt gccgtcaatc tcaaaaccat cgaagcccat
    21061 ctctttgata taacgaaatt tctccagaat attttccggg aagaacgcct gattctgtgt
    21121 gcctattttc attttttcat tcctttgaat taaaacgtga cgcccatttt gatgctctgt
    21181 tccggatgct ggtcgacata ctgcatgtag ctttccgggc tgttagcaaa ggtcaccacc
    21241 gggtcgatta aatcctcaca gttgagataa ccgttcatca gcagctccca gcaggtttct
    21301 tcgatacgct tgcggctcca gcgcggataa tccgggttcg gttcgctgca cgcgcgggag
    21361 aaaacaattt tggcgttatt gaaatgcgct tcgcgtccga ggttaaaacc ttcggcaaac
    21421 ggtttagcaa acgcgacata ggagatggtg ccaccgtaag ccaggccgcg cagcgccgac
    21481 tgcagcgcgt cggcgtaacc gctggtttcg ataatcacat cggcaccctg cttgccggtc
    21541 agcgttttga tctctttgcc tacgtcagtg ccaatgggat taaggcagaa atccgcaccg
    21601 tgacgacgag caatatcaca acgatgggca ataggatcga cgccaattac caccgacgcg
    21661 ccagcgcgtt tagccagttg gatggcaatt tgaccgatcg cgccaagccc taccaccact
    21721 acaaaatccc ctacgcgtac gttggcatcg cgaacgccac tcatggcgaa ctgcgccggg
    21781 tcgtagcaga cggcgttttt ccaggagctg ccttccggca ttttgcgcaa cttgtagtta
    21841 ttcactgcgt taatgatgac cgtctcggag agcgggccgt agccgcatac gctgtcgccc
    21901 accgcatagt cggtaacgtc gctgccgcac tcgataatgt cgccaaccac catgttgcca
    21961 agctggaatt tgccaaactc aatgccgcgc ggcgcacctg ccggacgcgg ggtgaacatc
    22021 tgccactcgc cgttaaagtc ttcatcgata aacgggctgg cggcgcggaa gtcgaccact
    22081 tccgttccgt gtttcggtgc gccgaaacgc acgcggatct tcacttcatt ggctaaaatc
    22141 gcccgatctt catactcaac cagcgcagca acacgcggtg ctgtggctac taactttttc
    22201 atgtaaaact ccttgtaaag aaactggccg ctttagccct tcacgccacc ggcggtcaaa
    22261 ccactcttaa taaaacgttc cgaaagcgca tacataatca ccaccggcaa tgcggtcacc
    22321 agcgaggccg ccatcatccg tccccagatg taatcaggcg tactgaacag cgcgttcagg
    22381 cccactggta aggtgaaatt gctggcgctg gagaggaaaa tcgacgcaaa cagatagtcg
    22441 ttccacgcca ccataaagca gtagacgaac accgatatca gcccggacat cgccaggggc
    22501 acggtgatac ggaagatgat ttgcagacgg ttgaggccat ccatcatcgc cgcttcttcg
    22561 atctcatcag ggatggtgtc gaagtaactt ttcagcataa acacggcggt gggcagagtt
    22621 tgcgtcacca tggtaatgat cagcgccatc tcggtgtcgt aaatgccaag tgcggtgata
    22681 attttgaaca gcggcaccac cagcaaaatg ccggagaaca tgtacaccgt gtaaaagctg
    22741 gcgttgatgg tcatccgacc tttaaagcgc aggcgagaaa gcgcgtaagc cccgagaata
    22801 ccgagaaata ccgccaccac ggatgaaacc accgacacca ccatgctgtt acggaagtag
    22861 tcgacaaacg ggaaaatcac cgggttaaaa atgtcgacgt aatgctccag cgtccactgc
    22921 tgtggcagca gcgtaggatg cagcgagatc gcctcttttg cgctcttgaa cgaggtcatc
    22981 agcatcacaa aaaacggaaa tagcgtgatg atgagaaaca gcgccagccc gcagtaaaaa
    23041 ccgatgcgac tgagtgtgcg tttatttgtt gccattgagg ttcacccgtt ttctggtcag
    23101 cagaatgaca gcgaaaataa tgatgaatag caccaccgag atcgccgccg ctttccctaa
    23161 gtcattaaag gcaaaggcgg ttttgtagag atagacacca agaatatcga ccttggttgt
    23221 cagcagataa acatcagcga acatgtagaa catccagatg gtgcgcaggg tcaccaccgt
    23281 cgccaggacc ggcataattg cgggcagcgt gacgatgcga aaccgctgcc aggcattagc
    23341 gccatccatc tctgccgctt cgtacagcga tttgtcgatg gtctgcaaaa tcgccagaaa
    23401 cgagataaag gcatatggga aatagcgcca gatggcgaac agcaccacca gcacaaagct
    23461 actgcccggg ttatcgaacc acagcggtgc ctgctcgtag agatgcagaa gatcgacgcc
    23521 gaggtagttc acaatgccgt agccgttgtt gaacatgtat ttccaggcga acaccagcga
    23581 aatggacggc gttacgtagg agaggatcac cagcgaacgc gcggttttgc gcaggcggaa
    23641 ctcgcggttg aaaaacatcg ccaccgccag cccgagcacg gtgctgcccg ccaccaccag
    23701 cgcggtatac cagaccgtca tccacagcga atgccagaag ccgggatcgg agaggatacg
    23761 cacatagttg ctcaccccaa caaacgttga ctcgatgttg ggattgagcg gcagacgtaa
    23821 aaaactgatt tcgatattcg acaccatcgg ccacgccacc agaccgccca gcagtaataa
    23881 gctgggcgcg agaagcagca gcgcaaaggg catatcggaa cgacctgaaa aaagcctgtt
    23941 cattgcggat tcctgtcctg ggtttagtgc tgttcgatca gttcatccag ttttttctgg
    24001 ctcgcttgca gcgtagtaga gagatcggct ttacccacgg tgacgttatg caccattgaa
    24061 ctcaccacgc cagaacccgt cacatcaccc atgcgggtaa agtttttatc ccctactgcg
    24121 ccaaaaacct gaatatttgg cagttcacca attaactgat tcggcagttc acccagcgcc
    24181 ttgatcacgt cgttgtcttt ccaggtggcg gtagtgacaa ccgctttgtt caccggcagc
    24241 gccgcgcctg gcgacatcat cacccagtcg gcaatgttgt ctgcctgctc cataaaggtg
    24301 acaaattttt ctgctgcttc ggtctcttcg gtcttttgtc cggcggtaat ggtcagcgag
    24361 gtcaacatgc cgtagaccgc agagtttttc tcggttggca ccacgaaacc gacgtttttc
    24421 gggtcgcctt ctttaattac agccggaagg atataggtgg agtaaatcgc catcggcgcg
    24481 gtgccgttca taaaggcgtc tttcacttcc atgatgtcgt tagaacccgg catggtgttg
    24541 gcagcaaggt cgcggtaata ggtcagcgcc tgcatcatct ctggtgtatc aagggtgatt
    24601 ttgccttcgg cgttaaagac gttggcctgg ttggataacg caaactggga gaaggattgt
    24661 tccgtcagca cgctttctgc cgtaggcagc gcaataccgt attttttatt cgccgggtca
    24721 ttcagtttct gtgcaacgtc cagcagctgt tgccagtttt tcggctcctc cagcccggct
    24781 tttgccagca catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg
    24841 gtccaggcac tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct
    24901 tcaccaacgt tgctgatgac cgtggcaacc gccttgcgat cgataagctg ctctttgtcc
    24961 ataactttgg cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa
    25021 agggtgatga ctttggtgtt ataagcgtct tcttccacgg gcacttgttt aacggtgata
    25081 cctgggtttt ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc
    25141 tccaccgaag aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct
    25201 gaaatcaggg cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga
    25261 tgttcctttt tatttaatcc acataacctg ccaggggcgc agcgttattt tattacccaa
    25321 tatatcaact tcactaatca gatcccgacc gtgcaggcca gtaatattta catgctgaat
    25381 atttttactg acattaaaca atccggtcag acaattaccc tctgcgttac ttctttgaat
    25441 acgcattacg gatgaattaa ccgtatcgat ggtaaaatta ttatccggat gaaactcgtt
    25501 atggctgcga cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt
    25561 agcttcatcg ttcagttctc gggttatctc ttcactgtga tattttttac ggttaatcgc
    25621 acggttatat ccgagttttt cgacacctgc gtaatcatta cgcgagccca gaatactttg
    25681 aatatatatc gctggcacac cggggaaact taacaaaatc gcatgggcaa ggataaacct
    25741 ggcgcaacgt tcttcatcgc tactctcgcg gcggcttaac gcatccatat aggtgacatt
    25801 tatttcatag ggactgcgcg taccgtcggg attatttttc cagtttacta atgcaccttc
    25861 ctgctgtaat gcctcgacca gcgctaatat ttcgctttct ggtaacaagc cccgcagtgg
    25921 gttcagcccg atgccatcgt gagaggcgag gaagttaaac caggtcgttt taccggaggg
    25981 caagctcaga ctttgcgccc actggcacag cgcctcgacg ttctgttttt gcaccgcatg
    26041 cagcaccagc ggcggcagcg agaactgata caccatatgg gcttcatcat cgccgttgcc
    26101 aaagtaagca atgttgtctt tatgcggaac attggtctcg gtaatgatca ctgtgcctgg
    26161 cgcgacgtta tcaataatcg accgtaacag tttgataatc agatgtgttt tttccagatg
    26221 gatgcagctt gttcccggct ctttccacat aaagccaacg gcatccagac ggacatactc
    26281 tgcgcccttt tccaggtagc acagcaatac atccaccatc gccagcaaca cttcagggct
    26341 acggtaattg aggtcaattt gatcgtcgct aaaggtggtc cataaatggc gcgtggaatt
    26401 atcatccatt tgaaacggcg ttaatagcgg taacgcacgc ggacgggtga cggcactgag
    26461 atcggtttgc ggatcaaccg caataaagaa atcttcaaaa cccggaagct gttgcaaata
    26521 gtttttaaac cattcacttt ttgccgacat atggttgcag acaaaatcaa acattaaatg
    26581 actgcattca ccgagctgct gaatatcctg ccactccccc gcttcactgg cgacctgatg
    26641 ataatcaatt accgaaaatc catcatcaga tgaccatgga taaaaaggta ataaatgaac
    26701 atgtgaaaaa atgctttgca gccattgatg gtaaaactga ttaaatgtgg gtagtggttt
    26761 taaatcatta ctgtgaaatt gatcggcata ggtaattaac acgacgtcac tttcatccca
    26821 gtgtttttta cgtcgctgtg taattaatcg tttcgcactc tcaagacgcg ttacaagttt
    26881 ctgtaaatga gttgaggtaa atgttccacc gtaaatttct gccaagtaat ccgtaatttc
    26941 ctgtttcatg tgcctttccg tggtagcggt cccatgaaac gagagactac tccgcagaaa
    27001 ataaactgtc aattgagcac aagggtttta gtcacgcagg aaatcatttt ttaccaggga
    27061 aaaagcgtat ttgtgagctg acgcaaataa tctataggga tgtaaataag gtgtgacaga
    27121 aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac cttcggcatt
    27181 gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc gctaaggatc
    27241 tcttttgcct gcccggactg gcgtccggca ttgcaataca ctttcacggt gtcgttttta
    27301 tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt gatggccccc
    27361 tgaacgtgct cctgctgata ctgttctgga acacgaacat cgatccagtg ttcagcggcg
    27421 aaaacgggca gtgaaaacac cagtgtcaga gcaagtaagc cttttttaaa catgtaagcg
    27481 tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa tttttgcgaa
    27541 actcccctca ccttttataa cgctgcgcca gtttattggc agcccgactt aacagcggtt
    27601 ccagcgccac agccagcaac attttcagcg gtcggcgagc aactgatttt atcgcccagc
    27661 ccgccacacc cgctgggcca tagcgcagtg cggtaagaag taccagcttg cctgctaatt
    27721 tgaaaccagg ctttaccttt tgcccggcct gttgccagcg agtattcata actttcctca
    27781 cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac gttccatctc
    27841 gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca attcgctgct
    27901 ggaaggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg caaatgacaa
    27961 aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca gcaccaccag
    28021 gatacgcacc agttttaccg gtacatcaaa atagttggca atcccggcgc agacgccacg
    28081 gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc ccgccattag
    28141 cgatccctcc agttcggatg ttctgcatca agaatagatt ccagcgcctg aatacgttcg
    28201 cgcatccgtt ttgcttcatc agccagttgc gctaatcgct gctgctcact ttgcgacaat
    28261 tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa aacaaacagc
    28321 acaaaaatgg ttaatggaat agccagaaat agcgcgctca taagtactcc ttagacgatg
    28381 agcggcggcg cgttcggacg ccgcctggat gttattattg attgtcttgc ttcattttgg
    28441 cttttaattg tgccagttgc tcgctgattg catcatcggc tttcagttcg gcaaactgat
    28501 cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa
    28561 ttcgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca ctgtccagct
    28621 gacgacgcac atcgcgcgac gagtttgccg cctgatggcg taacatcaat gcctgctggc
    28681 gagcacgggt ttcgctcaat ttgttttcca gctcgccgat ctctttcttc atacgcacca
    28741 gcgtatcgtc caccagcgtc acttcatgtt ccagggactt aatcagatcg gtcagtttct
    28801 gtttttcaat taacgccgca cgcgccagat cttctctctc tttcagtagt gccagttcgg
    28861 ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga gtcagctgtt
    28921 tcttttccgc cagcgcacgc gccgaagtag aacgtacttc aaccaatgta tcttccatct
    28981 cctggatcat cagacgcacc agtttctgtg gatcttccgc tttctctaac agagcgttga
    29041 tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatattg tcctcttgat
    29101 ttctgcgttc aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca attttttatc
    29161 tttttgattt ataaagatct gattgaagaa tcaacagcaa catgccagga tgaattagcg
    29221 aattacacta acaagtggcg aatttcatca tggcagaata caaagataat ttactcggtg
    29281 aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg ctggacaaac
    29341 cggtgctcat catcggcgag cgcggcacag gtaaagaact gatcgccagc cgtctgcatt
    29401 atctctcctc ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg ttaaatgaaa
    29461 atctgctgga ttccgaactg tttggtcacg aagctggcgc gtttaccggt gcgcaaaaac
    29521 gtcatccagg gagatttgaa cgtgccgacg gcggtacgct atttcttgat gaactcgcta
    29581 cggcaccgat gatggtgcag gaaaaattat tgcgcgtgat tgagtacggt gaactggagc
    29641 gcgtaggcgg tagtcagcca ttgcaggtga atgtgcggtt ggtatgcgcg acgaatgccg
    29701 atctcccggc gatggtcaat gaaggcactt ttcgcgcaga cctgctcgac cgactggctt
    29761 ttgatgttgt acaactgcca ccactgcgcg agcgcgaaag cgacattatg ttgatggcag
    29821 aacactttgc catccagatg tgtcgggaaa tcaagctgcc tctgttcccg ggttttaccg
    29881 agcacgccag agaaacattg ctgaattatc gctggccggg aaatattcgt gaattgaaaa
    29941 acgtggtgga acgttcagtg tatcgccacg gcaccagcga ttatccgctt gatgacatca
    30001 ttcttgatcc ctttaaacgg cgtccaccag aagaagccaa cgccgtttca gaaaacactt
    30061 cgcttccgat gctgccgctg gatttacgtg agtttcagat gcagcaggaa aaagagttgc
    30121 tgcaacacag tttgcaacaa gggaaatata accagaagcg cgcagctgaa ttgctggggt
    30181 taacctatca tcagttccgc gcgttgttga aaaagcacca gatttagcga agttgggcaa
    30241 tacaacttgc cggatgcggc gtgaatgctt tatccggcat acataatcaa tacgccgttt
    30301 gcaggcctga taagcgcagc gcatcagaca ttattgcgtt gggctattaa tcgctcagcg
    30361 catcctgcaa aattttcatt gccgcatcga attgcgcatc cgggatggtc agcggataca
    30421 ggaaacgaat cacgttgccg tatgcgccac aggtcagcag aagcagcccc tgcgccagcg
    30481 cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc ggatcgttaa
    30541 actctgccgc aatcatagac cccagaccgc gtaccgccgc gatggccgga acgctttctt
    30601 tggcatcaat cagcgtgttt gtcagacgct ggcccagttg attcgcgcgt tcgcagagtg
    30661 attctttgtc gataatgttg agcaccgcgt gcgcggcagc caccgccagc gggttaccgg
    30721 cgtaggtgcc gccaagcccg cccggcgcgg gtgcgtccat aatattcgcg ttaccgacca
    30781 cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat cgtcattaag tccggcttat
    30841 cggcgtaatg atccatcgca aacagtttgc cggtacgcgc aaagccgctt tgcacttcat
    30901 cggcaatcat cacaataccg tgttcgtcgc acaggcggcg aatggcggca accaattctt
    30961 ttggcgccac gttgaaaccg ccctcgccct gaaccggttc aaaaataatc gccgcgacct
    31021 gtttcgcttc gatgtctgat ttaaacaaac gttcgatggc gtccagggag tcctgcgttg
    31081 aaacgtcatg taaatctgac ggatacggca cgtgatacac cgaaccgggg aacgggccga
    31141 agccgatttt gtacggcgcg acctttccgg tcagcgccat ggtcatgtac gtacgaccgt
    31201 ggaagccgcc gctaaacgca atcacacctg ggcgtccggt atgggcgcgg gcgattttca
    31261 ccgcgttctc aaccgcttcc gcaccggtgg tgaagaacgc ggttttagcc tgcccgctta
    31321 ccggggcaag ggcgttgatt ttctccgcca gggtgacgta gctttcatac ggcacaatct
    31381 gatacgcggt gtgggtaaac tgttgcagtt gctgctccac cgccgcgacc agatcagggt
    31441 ggcgatgtcc ggtattcagc accgcaatgc ctgcggcgaa atcgatgtac tcgttgccct
    31501 caacatcctt cagcgtagcg ttttcagccg actgggcgaa gaagttacac atcacgccaa
    31561 ccccgcgcgg ggtggcagaa agacgacgct gatggaattc attgttgctc atgtttcaca
    31621 ccttttgact cttcactgtt aatcggaaat taaacgccca gacgatcgcg caggctgtaa
    31681 taagccgcgc ccatcgcggt aaacggcaca cgcagcgtgc gcccgccggg gaacgggtaa
    31741 tgcggcagat tggcgaaggc atcgaaacgt tcggcgtcgc cgcgcagcag ttcggcaatc
    31801 aaacgtccgg caagatgagt acaggtgacg ccgtggccgc tgtaaccctg catgtaatag
    31861 atgttggtat cgaggcgacc gaactgcggc atacgcgaca gggtcagcag gaagttgccc
    31921 gtccagcgat agtcgatttt cacgcccttc aactgcggga aggttttcag cagtttcggc
    31981 accacaagac gctcaacgtc atccgggtcg cgcgcgccgt acaccacgcc gccgccgtac
    32041 agcaaacggt tgtcggcggt aagacggtag taatcaagca gatagttaca gtcttccaca
    32101 cagtagtttt tcgggatcag cgaacgggct aaatcttctg acagccgctc agtggtgatc
    32161 acctgcgtgc cgcacggcat actacgcttc gccagttccg gctccacttt atcgcccagg
    32221 tacgcattcc cggcgacaat cacatacttc gccgtcacct ggcctttggt agtacgcacc
    32281 acggcgggcg tggtgtgctg gatctgcgtc accgcagaaa gctcatacac gcgcccgccg
    32341 ttcaggcgga tagcgtccgc ttcaccaatg gcgaggttca gcgggtgaat atgcccgccg
    32401 ctgtgatcca gcagcgcacc agtgtagcga tcgctggcga cctcgcggcg aatggcgttg
    32461 gcgtccagca gctccagctg tttattgccg tagcgttccc agttctcttt ctgctcttcg
    32521 agtgtggcga gctgtttatc attcatcgcc acaaacaggc cgccggggcg gtagtcgcag
    32581 tcaatctgat agcgtttgat acgttcgcgg atgatctcgc caccctcgaa catcatgctg
    32641 ccgagcatac gggcggtgtc catgccgtag cttttttcga tcacgtcgat gtcgcggctg
    32701 taggagttca caagctgccc cccgttgcgt ccgctggcgc caaagccgat gcgtgaggct
    32761 tcgagaacca ctacgtcaaa gcccgcttcc gccagatgta gcgcggaaga cagcccggta
    32821 tagccgccgc cgaccacgca tacgtcgcag gtgattgact cattcagtgt gtcgaatggc
    32881 gcgtatttat tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat ttcaggcctc
    32941 caggcttatc cagatggttt tcagttcggt gaatttttca agggcatgca gggatttgtc
    33001 gcgaccgttg ccgctctgct tatagccgcc aaacggcacg gtcatatcgc cgtcgttgta
    33061 gttattgacg aagacggaac cggctttcag gcgtcggctc atgcggtgcg cgcgggagag
    33121 gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg tcgttggcaa gctgtagcgc
    33181 ctgttcttct gatgtgaaac gcgtgaccac cagcaccgga ccgaaaatct cttcgcggct
    33241 taaggacgca ttcgggtcta aatccacaaa gatggtcggg ccgatggcgg cagccagccc
    33301 ggcgttacgg ccatccaaca acagttgccc tttgctttcg ccttcccgaa taaagctatg
    33361 gaccgagtcg gcgtgggcgc agtcgattaa ggtgcccatg gtggttgcgg gatcaagtgg
    33421 atggcccggc tgccagtttt gcgcctgctg ttttaacagg gctaagaatt catcggcgat
    33481 gctctcttcc agcaacaggc gcgttccggc gatacacacc tgcccctggt tgtagaaaat
    33541 gcccgcagcg gtcgcactcg ccgctttttg cagatccggg cagtcagcga aaacgatgtt
    33601 ggcgcttttg ccacctgctt ccagccagac gcgtttcatg ttgctgtcgc ccgcatcttt
    33661 cagcagctgt ttcccggtac gggtcgaacc ggtaaaggcg atggcatcga tatcgttatg
    33721 gcgcgacagc gcctgccccg cttcatgacc aaaacccgtt actacgttca acacaccatc
    33781 cggcaagcct gcttctttcg ccagccccgc gagacgaatc gcactgagcg gtgatttttc
    33841 agacggtttt agaatcacgc tgtttcccgc cgccagcgcc gggccaagct tccagcaagt
    33901 cagcagcagc gggaagttcc acggtacaat ggcggcaatt acgccgaccg gttcacgcac
    33961 gatcatcgcc agctcatggc tactggtggt cgccacttcg ccatacactt tgtcgatcgc
    34021 ttcggcgtac cagcgaatgg cgcgcgccgc gccgggaata tcatctcgca gactgtgacg
    34081 aatcggtttg ccggtgtcga gagtttccag cagtgccagc tcttcggcgt tggcttccat
    34141 taaatcggcg agtttattca gcaccgcttt acgtttagcc ggagaagaga gtgaccagtc
    34201 gccgcgttca aatacgccgc gtgctgcgct caccgcacgg tcgatatcga cgctcttgcc
    34261 gcgggcaatt ttcgccagcg gtgcctgggt gaccggatca acggtttcaa aggtttcatt
    34321 ttccgccgca gcagtatatt caccgttaat aaataagcgg gtttcaatgg cgagacttaa
    34381 cgctttatcc tgccagtaag ccagatgatg aaaattcatt atgactcctg tttcacgact
    34441 atcagatata tgcagtttgt gaacgtcagg gcgcagagat ccccgggccg tatgcccgtt
    34501 tatttctggc gacattcttt atggattgcc gggcgcattg cgcttacccg gcttacagaa
    34561 caaaaagatt aaaacgtggt gggcgtatgg gcgctaataa ttcggcaaat acctgccgat
    34621 gtattactga aactgtgcgg gatgccggta ttaatggcat aactttgccc cgcgacgaga
    34681 tggtaatcct gaccattaat cgtcagaaca atttcacctt ccagtacagt gcctatttcc
    34741 tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtacgt ttcaaagatc
    34801 atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac accctgactg
    34861 cccatctcaa ttaaatcgtc ctgattaatg acgacctgcg gctcatcagg tttttccggc
    34921 tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg cagcgtactg
    34981 atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt cagcccggag
    35041 agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga caagcgtttt
    35101 cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt ggtcagagtc
    35161 gctgtttttc agcgatatgg tgctgacaag cggtgataaa gccctcgaac aatatacgcg
    35221 aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgcccagc gcaaaaggat
    35281 gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca acacgcaacc
    35341 gtgggctaac gaccttcgcc ccttgtccat gcagagagtt tacccaaaag ttgctacatt
    35401 caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc gacggtgcat
    35461 attgctgttc caccggcagt tcgggatctt cccgatgttc cagcaattca ggctgttcgc
    35521 acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcctgtaaa ccccggcaga
    35581 tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat taacgccatg ctcagaagat
    35641 cacgcccggg atcggcgtca ggctcatcgc cgttttcacc atataggtgc ggctgcacat
    35701 tgctgggact accaggaaga taaatgccat cgagtttcgg caaaagttgt tcaagtaagg
    35761 acggttccgc cagcgcatgt ggcagcgcaa ttggcaagcc gcctgcatgg atgatggcat
    35821 tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc ctgcacatta
    35881 cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca aagtggacta
    35941 aattatcgcc attactgcgg tttaaatgac ctttatgttc aatatttttt caatctagca
    36001 gtgggttttc atttttgcaa actcaattta acatttgaca aacatttagt ttgcatacag
    36061 attcgaatgg tggtcattat attttacgct ttgataacga gcggaaaaca aaccaaaggc
    36121 gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag tcagaagaga
    36181 ggcggggtag cgcctttacg caggaagtga aacgctacct ggagcgctac ccgaatacgc
    36241 aatatgttga tgttttactt accgatttga atggttgctt ccgtggaaaa cgtattcctg
    36301 tttcaagcct gaagaagctc gagaaagggt gttatttccc ggcctcggta tttgcaatgg
    36361 atattctggg caacgtagta gaagaagcag gtctgggtca ggaaatgggc gagccggatc
    36421 gtacctgtgt tcctgttctc ggttccttaa ccccttctgc cgccgatcca gagtttatcg
    36481 gtcagatgct cctgaccatg gtcgatgaag atggcgctcc ctttgacgtt gagccgcgga
    36541 acgttcttaa ccgcctgtgg cagcagctgc gccagcgcgg attgttcccg gtcgtagcgg
    36601 tagagctgga gttctattta ctggatcgcc agcgcgacgc tgaagggtat ctgcaaccgc
    36661 cctgcgcgcc cggcactgat gaccgcaata cacaaagcca ggtttactcc gttgataacc
    36721 tcaaccactt cgccgacgtg ctcaatgata ttgatgaact ggcgcagtta cagctgattc
    36781 cggcagatgg cgcggtcgct gaggcctcgc cgggtcagtt tgaaatcaac ctttaccata
    36841 ctgataacgt gctggaagcc tgcgatgatg cgctggcact aaaacgcctg gtgcgtctga
    36901 tggcagaaaa gcataagatg cacgccactt ttatggcgaa gccgtatgaa gagcacgcgg
    36961 gcagcggaat gcatatccat atcagtatgc aaaataatcg tggcgagaac gtgctttctg
    37021 acacagaagg cgaagattcg ccgctgctga aaaagatgct cgccggaatg atcgatctga
    37081 tgccgtcatc aatggcgttg ttggcaccaa acgtgaactc gtatcgccgc ttccagccgg
    37141 ggatgtatgt accgacgcag gcgtcgtggg gccataataa ccgtaccgtc gccctgcgta
    37201 ttccatgcgg cgatcgtcat aatcaccgcg tggaatatcg cgtggcgggt gccgatgcca
    37261 acccatatct ggtgatggca gcgatttttg ccggtatttt gcacggtctt gataacgagc
    37321 tgccgttgca ggaagaagtc gaaggcaacg ggctggaaca ggatggctta cccttcccga
    37381 ttcgccagag cgatgccctg ggtgagttta tcgagaatga tcacctgcgc cgctatttag
    37441 gcgaacggtt ttgccatgtg tatcacgcct gtaaaaatga tgagctgttg cagtttgagc
    37501 gcctcatcac agaaacagaa attgagtgga tgttgaaaaa cgcatagtac cccttgcggg
    37561 gttgtttcat cggcctgagc ggagccaggc ctgacatttg ctggtaatcc agacatttcc
    37621 cggatgtcgc gttatggagc gcaggcggca acgggcttcc gttaacgatt aagactgtga
    37681 cgaggaaatg agatgatgcc gatgattaaa tccccgcatg gcgagggagg ctgcgtatgc
    37741 gcccctcctg cgactgactg gactccgccc cctttgcttc ccttgctaaa caggtttgat
    37801 ttcaggtcga cacgaccgca aaccttatta cgcaggggag gcagcaatta tggctattaa
    37861 ttcaccactg aatattgctg cgcaacccgg caaaacccgt ctgcgaaaat cactgaaatt
    37921 gtggcaggtg gtgatgatgg gtctggccta tctcacgccg atgactgtgt tcgatacctt
    37981 tggcattgta tctggcatta gcgacggtca cgtcccggca tcctatttgc tggcgctggc
    38041 gggcgtgctg tttaccgcta tcagctacgg caaactggtt cgccagtttc cggaggccgg
    38101 ttcggcctat acctacgcgc aaaagtcgat taatccgcac gtcggattta tggtcggctg
    38161 gtcatcactg ctggattatc tctttttgcc gatgatcaac gtactgttgg caaaaatcta
    38221 tctctccgcc ctcttcccgg aagtgccgcc gtgggtgtgg gtggtgacct tcgtcgccat
    38281 tttaactgcc gcgaacctga agagcgttaa cctggtcgcc aacttcaata ccctgtttgt
    38341 gctggtgcaa atctccatca tggtggtgtt tatcttcctg gtggttcagg gactgcataa
    38401 aggagaaggc gttggcaccg tctggtcact tcaaccgttt atcagcgaga acgcgcacct
    38461 gatcccgatt attaccgggg cgacgattgt gtgcttctcg ttcctcggtt tcgatgcggt
    38521 gaccacgctt tcggaagaga cgccggacgc cgcacgcgtg atcccgaaag ccatcttcct
    38581 gacggcggtc tacggtggcg ttatctttat cgcggcgtcg ttctttatgc agctgttctt
    38641 tcccgatatc agccgcttta aagacccgga cgctgcactg cctgaaattg cgctgtacgt
    38701 cggcggcaag ctgttccagt ctattttcct ctgcaccaca tttgtgaaca cattggcctc
    38761 tggcctggct tcacatgcca gcgtgtcgcg cctgctgtat gtaatggggc gtgacaatgt
    38821 gttcccagaa cgtgtgtttg gctatgtaca cccgaaatgg cgtaccccgg ctctgaacgt
    38881 cattatggtc ggtattgtcg cgatgtcggc attgttcttt gatttagtca ccgcgacagc
    38941 attgattaac ttcggtgctc tggtggcgtt taccttcgtg aatctgtcgg tgtttaatca
    39001 tttctggcgg cgtaaaggaa tgaataaaag ctggaaggat cacttccact atttgctgat
    39061 gccgctggtt ggcgcgctga cggtgggtgt gctgtgggtt aacctcgagt caacgtcact
    39121 gacactcggt ctggtatggg cttcgttagg cggcgcatat ttgtggtatc tgatccgccg
    39181 ctatcgcaaa gtgccgttgt atgatggtga cagaacgcca gtgagcgaaa cgtaatagtc
    39241 ggatgcgcgg cgcattatcc tgcctgaggg taatgcgccg cgccattaac tataaaaaga
    39301 tgtatgaccc ggatcacgag tgctatcatc cgcccttatg cttagcgcat taaccccagg
    39361 ggcaaataat gaaccacgat atcccgctaa aatattttga tattgccgat gagtacgcga
    39421 ccgaatgcgc agagcctgtc gcagacgctg aacgcacacc gctggcccac tacttccagt
    39481 tactgctcac ccgtttgatg aacaatgaag agatcagcga agaagcccaa catgaaatgg
    39541 ctgctgaagc ggggattaat cccgtacgca ttgatgagat cgcagagttt ttgaatcaat
    39601 ggggtaatga gtaattattt aattgcagac gatcggtcag tatcgttgtc tggataaagc
    39661 gttcacgccg catccggcta acaatgacac gcactgcagc gtgctttaat gggtttctca
    39721 cgatcgtacc gctctgctcc tcgctaagtt gttctgtgag ttatagttat agagaaatca
    39781 cgggtacgaa tgttgaacag atggcgacgc cacctgttca acgaccaacc acacggccct
    39841 cattacgacc cctgaacaca aacggtaatg gcaaatgtca ttaaatatta gtaagatagc
    39901 ttctttacat aaaatccctt aatattatgc gccaggtatt atcgtctctt ttggtgattg
    39961 ctggacttgt gagtggtcag gcaatcgccg cgcctgaatc ccccccgcat gctgatatcc
    40021 gcgacagcgg ttttgtctat tgcgtcagcg ggcaagtcaa cacctttaac ccatccaaag
    40081 cgagcagtgg gttaattgtc gatacccttg ccgcccagtt ttatgatcga ctgctggatg
    40141 ttgatcccta tacctatcgc ctgatgccag aacttgccga aagctgggaa gtactcgaca
    40201 acggcgcgac ctatcgcttc cacctacgtc gcgatgttcc gtttcaaaaa accgactggt
    40261 ttactcccac tcgtaaaatg aatgccgacg atgtggtgtt taccttccag cgaatttttg
    40321 accgcaacaa cccgtggcat aacgtcaacg gcagcaactt cccctacttc gacagcctgc
    40381 aatttgccga taacgtgaaa agcgtccgta aactggataa tcataccgtt gagttccgtc
    40441 tggctcagcc ggatgcttct tttttgtggc acctcgcaac ccattatgct tcggtcatgt
    40501 cggcagaata tgcccggaag ttagagaaag aagatcgcca ggagcaactc gaccgtcaac
    40561 cggtcggcac tggaccgtat cagttgtcgg aataccgcgc cgggcaattt attcgcctac
    40621 aacgtcatga tgacttctgg cgcggtaaac cgttaatgcc gcaggtggtg gtggatttag
    40681 gctccggcgg caccggacgt ctgtcgaaac tcctgaccgg ggaatgcgac gttctggcct
    40741 ggcctgctgc cagccagcta tccattttgc gtgacgaccc gcgcttgcgt ttaacgctgc
    40801 gtcctgggat gaacgtcgcc tatctggcat ttaacaccgc caaaccgccg ctaaataatc
    40861 ccgctgtccg ccatgcgctg gcactggcga ttaataacca gcgcctgatg caatccatct
    40921 attatggtac ggctgaaacg gcggcctcta ttttaccgcg cgcctcgtgg gcctatgaca
    40981 acgaggctaa aattactgaa tacaatccgg cgaaatcgcg cgaacagttg aagtcgttgg
    41041 ggctggaaaa tttaacgctg aaactgtggg tgcccacacg ttcgcaggcg tggaacccca
    41101 gtccactgaa aactgccgaa ctgattcagg cggatatggc gcaggttggc gtaaaagtgg
    41161 tgattgtgtc ggtagaaggt cgctttcagg aggcgcggtt gatggatatg agccatgatc
    41221 tgacgttatc cggttgggcg acggacagta acgacccgga cagtttcttc cgtccgttac
    41281 tgagctgcgc ggcaattcat tcacagacca acctcgccca ctggtgcgat ccgaaattcg
    41341 acagcgtgtt gcgtaaggcg ctctcctcgc agcagctggc ggcgcgtatt gaagcctatg
    41401 acgaagcgca gagtattctg gcgcaggaat tgcccatttt gccgctggcg tcgtcattgc
    41461 gtttgcaggc ctatcggtac gatatcaaag gtctggtact tagcccgttt ggtaacgcct
    41521 cctttgctgg ggtgtatcgc gagaaacagg atgaggtgaa aaaaccatga ttatcttcac
    41581 cttacgccgc attttgttat tgattgtcac cttgttcctg ctgaccttcg ttggcttcag
    41641 cttgagctat ttcactccac acgcgccgtt acaaggcgcg tcactgtgga atgcctgggt
    41701 gttctggttt aacggcctga tccactggga ttttggtgtt tccagcatta acggtcaacc
    41761 aattgccgaa cagttaaaag aggtcttccc ggcgacgatg gaactgtgca tcctcgcctt
    41821 cggttttgcg ctgattgttg gcatccccgt ggggatgatt gccgggatta cgcgccataa
    41881 gtggcaggat aacttgatca acgccatcgc cttactgggc ttttcaatcc cggtgttctg
    41941 gctggcgctt ctgttgacgc tgttttgttc actcacgcta ggttggttgc ccgtttcagg
    42001 gcgtttcgat ctgctctacg aagtgaaacc gattaccggt tttgcgttga ttgatgcctg
    42061 gctttcggac tcaccatggc gggatgaaat gatcatgagc gcaatccgcc atatgatatt
    42121 gcccgtgatc accctgtcgg ttgcgccaac aactgaagtg atccgcctga tgcgtatcag
    42181 cactattgaa gtgtatgacc agaactatgt taaagcggcg gcgacccgcg gtttgtcgcg
    42241 ctttactatt ttgcgtcgcc acgttctgca taacgcgtta ccgccggtta ttcctcgcct
    42301 gggcttacag ttttccacca tgctgacgct ggcgatgatc accgaaatgg tttttagctg
    42361 gccgggttta gggcgctggt taattaacgc catccgccag caggattacg cagccatttc
    42421 cgccggagtg atggtgtgtg gctcactggt cattattgtt aacgtgattt ctgatatttt
    42481 gggtgccatg gctaaccctc tgaaacataa ggaatggtat gccttacgat agcgtataca
    42541 gcgaaaagcg cccgccgggc acgctgcgta ccgcctggcg caaattttat agtgacgcct
    42601 ctgcgatggt cggcctgtac ggctgcgcgg ggctggctgt actgtgtatt tttggcggct
    42661 ggtttgcgcc ttacggcatc gaccagcaat ttctcggtta ccaattattg ccgccgtcat
    42721 ggtcgcgcta tggcgaagtt tctttcttcc tggggactga cgacctcgga cgcgatgttt
    42781 taagccgttt actgagcgga gctgcgccaa ccgtgggtgg cgcatttgtg gtgacgcttg
    42841 ccgcgacgat ctgtggcctg gtactgggca ccttcgccgg ggcgacacac ggcctccgct
    42901 cagcggtgct taaccatatt ctggatacct tactggcgat cccttccctg ctgttggcaa
    42961 ttatcgttgt agcgtttgcc ggaccgagtt tgtcccacgc tatgtttgct gtctggctgg
    43021 cattgctgcc gcgtatggta cgttcgattt acagcatggt gcatgacgaa ctggaaaaag
    43081 agtacgttat cgccgcccgt ctggatggcg catcaacgct gaatattctc tggtttgctg
    43141 tgatgcctaa catcaccgcc gggctggtca ccgagatcac ccgcgcactg tcgatggcaa
    43201 ttctcgatat cgccgcgctg ggctttctcg atctcggcgc acaactcccc tcgcctgaat
    43261 ggggagcgat gctcggtgat gcgctggaac tgatttatgt cgccccgtgg actgtcatgc
    43321 tgccaggtgc ggcaattatg attagcgtgc tgttggttaa cctccttggc gacggtgtcc
    43381 gtcgtgcaat tattgcgggg gtggaataat gccattactc gatattcgta acctgactat
    43441 tgaatttaaa accggggatg agtgggttaa agccgtcgac cgggtaagta tgaccttaac
    43501 cgaaggtgaa atccgcggtc ttgttggtga atccggttcc ggcaagagtt tgattgcgaa
    43561 agcgatttgt ggggtgaata aagataactg gcgtgttact gctgaccgta tgcgttttga
    43621 tgatatcgat ttgctgcgtc tctccgcacg cgaacggcgc aaactggttg gccataacgt
    43681 gtcgatgatt ttccaggaac cgcagtcgtg tcttgaccct tcagaacgtg tgggtcgcca
    43741 gttgatgcaa aacatcccag cctggaccta taaaggccgt tggtggcagc gttttggctg
    43801 gcgcaaacgg cgtgccattg aactgctgca ccgcgtgggg attaaagatc acaaagatgc
    43861 gatgcgcagt tttccctatg agttgaccga aggtgaatgt cagaaagtga tgatagccat
    43921 tgcactggcg aatcaaccgc gtctgctgat tgctgacgaa ccgacaaact caatggagcc
    43981 aacaacccag gcgcaaatct ttcgcctgct gacgcgtcta aaccaaaaca gcaataccac
    44041 tattttgctt atcagccatg acttacaaat gcttagccaa tgggcggata aaattaacgt
    44101 gctttactgc ggtcaaacgg tggaaaccgc gccgagtaag gagttagtga cgatgccaca
    44161 tcatccttat acccaggcgc tgatccgcgc gataccagac ttcggcagcg cgatgccgca
    44221 taaaagtcgc ctcaatacgc tacccggcgc tatcccactg ctggaacagt taccgattgg
    44281 ttgtcgtctg gggccacgtt gcccatatgc acaacgagaa tgcattgtga cgccacgttt
    44341 gacgggggcg aaaaatcatc tctatgcctg tcatttcccg ctgaacatgg agaaagagtg
    44401 agatgatcga aacgctgctt gaagtgcgta atttaagtaa aacgttccgc taccggaccg
    44461 gttggtttcg tcgccagacc gtagaagcgg taaaaccctt gagctttacg ctacgtgaag
    44521 gccagacact ggcgattatt ggcgagaatg gttcgggtaa atccacgctg gcaaaaatgc
    44581 tggcgggaat gatagaacct accagcggcg agttattgat tgacgatcat ccactgcatt
    44641 ttggcgatta ttccttccgc agtcagcgta ttcgcatgat ttttcaggat ccttcgacct
    44701 cgttgaatcc ccgtcaacgt atctcgcaaa ttctggattt tccactgcgc ctgaacacca
    44761 acctggaacc ggaacagcgt cgcaaacaga ttattgaaac gatgcgtatg gttgggctgc
    44821 taccggatca cgtcagttac tatccgcata tgttggcccc cgggcaaaaa cagcgtctgg
    44881 gtctggcgcg cgcgctgata ttgcgcccaa aagtcattat tgccgatgaa gccctcgctt
    44941 cactggatat gtcgatgcgt tcgcagttga ttaatctgat gctggaatta caggaaaaac
    45001 agggcatttc gtatatttat gttacccagc atatcggaat gatgaagcac attagcgacc
    45061 aggtgctggt gatgcatcag ggcgaggttg tcgagcgagg cagcaccgcg gatgtgctgg
    45121 catcaccgct gcatgaactc accaaacggc tgattgccgg tcattttggc gaggcattga
    45181 cggcggatgc gtggcgtaag gaccgttagt gttctgcacc tcatcctgac aggggccccc
    45241 cctcaggatg aggtattcat tacttttgat tgtcgattct ggtgagagca accaggaatg
    45301 ccagtgtata acacaccgta attgccagac tcatttttaa cagtgtttct ccgccaaatc
    45361 ccgacaatgg cgcggctata ccgccgaaga caaacatgct catccccatc aacgcagacg
    45421 ccgttccaga ttcctgtgtc cctactgcac tcatagcctc tgccccgcca acagtgccga
    45481 tgccgctaca aaatgcaata gtgaaaaata atgccaccag tgccggaatg ggcaattgtg
    45541 tccatgcgca taagaccgtg agcaatgcac acaaaattgc cgctatcagg ccacctcgca
    45601 acaatgtcat cgcattaatc cgtcgcgcca gacgcgaaaa gatccatgaa gcaatgataa
    45661 gtccgatgcc gttaaggcca aacaccaggc taaattgcat tggactaaag ccaaattcct
    45721 tctgcaacac gaacgacgaa gagccgatat acgcaaacaa gcctgccagc ataaaacttt
    45781 gaatcaggca aaagcgcata aaacggcggt tctgcaccac gcttcgtccg gttaatagca
    45841 atgatgagcc ccttttattt tctggcaagg tctcattaat aaataacacg cagcccagca
    45901 acaatacggt gctaatttca gccattaccc agaataaagt gcgccagtca aaagtgctaa
    45961 caatgtaccc gcccagcact ggcgacaaca ccggtgccag gccattcacc gtcattaaca
    46021 gcgcaaaaaa ctgggtcaac gttactccct gatatttgtc acgagcaata gaacgagaga
    46081 gcaccgaacc acccgccccc gcgatccctt gaataaaacg ccagaccacc agccagtaaa
    46141 tattgttcgt tgtcgcgcac aaaatggaag aaacaataaa caatagcaac gacaagatta
    46201 acggacgttt acgccctatt ttgtcgctca gagggccaaa taacagttgt ccgacgccaa
    46261 gaccgatcag tgatgcagtc agcgttaatt gcgttatggt cgtggttgcc gccagttgct
    46321 cagacatctc cggcaaagcc ggcaaataaa gatcggtaca catcgggccg ataccggcta
    46381 aaagaccaag aatcaatgcc catgaaggag agactctagc catattattc cttacttgag
    46441 tgatgacatg ccgccgccca gcgactgcca gagcgtgatt cggtttgtaa aatcagtcag
    46501 ttgcagtgcc agcagctcct gctgtgcgct ccacaagctg cgctgcgccg tcaacacggt
    46561 caggtaatca ccaacacccg tctgatagcg acgcaagcca acatcgaccg tttgttgctc
    46621 agctttaaca tactggcgct gtgcatccag ttgctcttca agcgtggtcc gccgcgccag
    46681 tgcgttagcg acatctttaa atgcgctctg aacggttttt tcataggtgg caatgagtcc
    46741 acgcttttgt gcttctgcgt aacgaagctg cgccagatta cttccgccag tgaacagcgg
    46801 taatgtgacc gatggcgtaa atgaccagat ctgcattccg tggctgaaca acgacgacaa
    46861 tgcgtcactg ccaacaccag cgctggcagt cagcgaaatc gtcgggaaaa agttagcccg
    46921 cgccgcgcca atatcggcat tggcgctctt caaattatgt tccgcttcct ggatatccgg
    46981 gcgacgcagc agaacgtcag atgacactcc cgcaggaacc agacttatca tctgttccgg
    47041 caggctttcc agtgttcccg gtagcaaatt ttcctcaagc gttgtacctg ccagtaagtt
    47101 cagcgcattt ttatcctgca tcacttgtgt ctgatagctg gcaacgctgg cgcgcgcttg
    47161 ctgataaaca ctcatcgcct cactgacatc tgtcgctgcc gccgtgccaa cctgttgctg
    47221 gcgctggata attttcaatg agttttctgc actggtcatc gtttcttttg ccagggccag
    47281 gttgctgtta tcggccgcca gtgtcagcca ggcggtgctg atttcagcaa tcagcgttaa
    47341 acgggtgttc tgggctgtaa attcactggc aagccaggtt tctcgcgccg cccgagataa
    47401 actctgatta cgaccaaaca gatctaacgt aaaactggac accgtgccgt cagcctcggc
    47461 agtggtttcc gtaccattcg ccagcgaacg actgcgggtt gaacttaagg ccgcattcac
    47521 cgtcgggaat aacgacgcat tagtttgccc atagagcgcc cgtgctgagt caatatcagc
    47581 aatcgctttt tgcacatcgc ggttactgtt cagtgcaata gtcaccacct gctgcaatcg
    47641 gggatcgtgg atcacctgct gccagtcatg gctgattgcc tttccctggc cctgtgctcc
    47701 cggcaaggtc gccgggattg gggattcagg cgtgctgtag tggggatcga gcgagacaca
    47761 tcccgcactc aacagcacca gaaaaataag agaacgacgc aacataactt actcctgacg
    47821 gcgcgattta ccggcaaaca aacgtttcac cagtacaaaa aacagaggaa caaagaaaat
    47881 agccagcaac gtagcggtca gcgtaccgcc aataatgccc gtaccaatgg cgatgcggct
    47941 gttcgcccct gcgccggttg ctatcgccag cggcattacc cccgcaataa acgccagcga
    48001 ggtcatgatg attgggcgta aacgagtctg cgccgcgcgt aatgccgcac gactcaagga
    48061 ataaccctct gcaaccgccg cttcagcaaa ttccacaatc agaatggcgt ttttcgacga
    48121 caagccgata gtggttaata gcgccacctg gaagtaaaca tcgttgttta aatcacgcat
    48181 ccaggccgcc agcgccgcgc caagaagccc cagagggatc accagaatga ccgagaacgg
    48241 gactgaccag ctttcgtaca gtgctgcaag gcagaggaaa accaccagaa tagaaactgc
    48301 atacagactc aatgcctgac cgctggccag tttctcctgc aatgacaaac cactccaggc
    48361 ccaggttgtt cccgcaggaa gactgtttgc cagttcctcc atttttgtca ttgcatcgcc
    48421 ggaactaaag ccagtcgcat tttccccctg aatttcatag gctgcagaac cgttgtagcg
    48481 caccagtctt tccggtccat acagccagcg ggtggtcgca aaagcagaga atggtgtcat
    48541 ggcgttatca ctaccgcgca caaaccattt acctaagtct gacggcgcgg aacgaaattc
    48601 actgtcgccc tgaatgtaga cttttttcac acgaccacgg tcaataaagt cattcacata
    48661 agtaccgccc cacgcactgg acagggtgtc ggtgacatca ttcaaactca accctaatga
    48721 caccgcttta ttactatcaa tatctacctg caattgcggc atttgtggca aatcattggc
    48781 acgaacagaa tgtaactccg gactttgatt cgctttttct atcaactgat tacgcatttg
    48841 cagtagtgtt tcgcgatcgg ttccaccgtt agctaacaat tcaaacgtaa aaccattgct
    48901 ttgccccagc ccatcaacgg ctggcggcgt catcgcaaat accgtggcat cacgaattgt
    48961 gcccagctct ttggttgccc gtagggcgat agcctgggcg gtgttttctg cccctttacg
    49021 ttgagaccag tttttcaacg aaacaaacgc catcccggtg ttctgtccgc tgccgctaaa
    49081 actgaaacca tcaacggtaa aaatgacatc ggtatttgct ttctcgttaa tcaggaacca
    49141 gtcaacaatc tggcgattca cttctgctgt acgggcagcg gtcgccccgg ccggcagcgt
    49201 gtactgcacc atgatttcgc cctggtcttc agtgggtaag aaactgcccg gcagtttcca
    49261 catcatcagg gccattccac cacccagtac gagataaagc cccatcgttc gagcagcacg
    49321 gcgcaggaca taaattacgc ctcgctgata tttatcttca gtacggcggt aaaagcggtt
    49381 aaatgcgccg aaaaagcctt ttttatgtgg cggaacatgc tggaggacgg aaccgcacag
    49441 ggcgggagtc aaggtcaatg ccaccaccac ggaaagcagc attgcggaga taatggttat
    49501 cgaaaactga cgataaatta cccccgtaga tcccccaaag aaggccatag gcaggaatac
    49561 ggctgacaac accagcgcaa tggcaaccag tgcgccagaa atctcgccca ttgatttttc
    49621 cgtggcttca cgcgcgggca acccttcgtc acgcataatg cgttcgacgt tctccactac
    49681 cacaatggca tcgtccacca gcaagccgat cgccagcacc attgcaaaca acgtcagggt
    49741 attaatcgaa tagccaaaca acgcaagaac gccgaatgtg cccagcaaaa cgacggggac
    49801 agctaacgcc ggaatcagtg tggcacgcag gttttgcagg aataaataca tcacgcaaac
    49861 caccaatacg atggcttcaa acagcgtctg aattacgtcc tctacagaga ttttaataaa
    49921 ttcagtgctg tctttcgggt aagcgatgtc gtatccctgc ggcatgttcc gctggaattc
    49981 ggcaatctta tccttgacca gcgtcgccgt attcagcgca ttcgcaccgg gcgaaagcat
    50041 tacagcaacc ccggcagacg ggtgaccgtt aagtttgccg atagcggtat aatcttcact
    50101 gcccatctca acgcgagcca catcttttat acgaacaact gcgccgtctg actggctttt
    50161 cacgataata tttttgaact gatccaccgt ctgcaagcga gactgcgcac gtaccgttgc
    50221 ggtcagttgc tgagtattcg gtgaaggcaa tgcccctatt ttcccggcag taacctgcac
    50281 gttttgcgct tcaatagcac tttgcacgtc tgaaggcatt agcgagtaag acgccagttt
    50341 ggccgggtca agccagatgc gcatagcgta ttccgcgcca aagacttgca gactccccac
    50401 accttcaaca cgcgccagcg ggtcctgaac gttactaacc aaccagtcgg cgatatccga
    50461 actggaggct ttgtcagtgg tgtcatacac ggcagcaatc agcaaaaagt tactttgtga
    50521 tttctccacc gtaacacccg tttgctgcac ttcgctgggt aggcgcgact ccgcctgctg
    50581 aattttattc tgcacctgaa cctgtgcagt atctggatcg gtaccttgtt caaaggtcac
    50641 attaatactg accgaaccat cagagctgct ggttgagctg aaatagagta aattatcaag
    50701 cccagtgagt tgttgctcga taacctgagt cacgctgttc tccagcgttt cggcagaagc
    50761 accagtataa gtggctgaaa ttttaatggt cggtggcgca acgtcaggat attgcgcgac
    50821 gggcaatgtg cgaatagcca gaatccccgc caacataatc aaaatggcga taacccaggc
    50881 aaagaccggg cggcgaacaa aaaagcgcga aaacatcagg cgttgcctcc attagcctga
    50941 acttcaacag ccttgaccgt ctgccctgaa gtgacttttg cagaaccttc aacaatcagt
    51001 cggtcgccgt tgtgcaggcc gtttagcacc agccatttat caccatatgt ttctcccgtt
    51061 tcgagcgttc gctgctctac tttattgtct ttattcacca ccagcgcagt tgcattgcct
    51121 ttagcatcgc gcgtgacgcc ctgttgcggc gcgagaatag cgtcttccat cacgccttca
    51181 tcgacacgag cgcgaacaaa cattcccggt aataactgct gttgtggatt ggggaaaatc
    51241 gcccgtaatg tcaccgaacc ggtagactca tctacagcga cttcggtgag ttccagacgc
    51301 cctttttcgc tgtaggttgt gccatcttcc agaataagtg agacgctcat ggtgtcactg
    51361 ttggtcgcca gtgactgttt acgcagacgt aataaatcga cactggagcg agtgaggtcg
    51421 acatacattg tatccagacc acgaatagtc gttaacgctg tatcttgcga cgcggtcacc
    51481 agtgcgccag gggttaccga cgaaatgcca atgcgccctg aaatcggtgc ggttaccgtg
    51541 gtccagtcaa gattaatgcg cgcagtttcc agtgcggctt ttttcgcctc tacactggct
    51601 ttatcttgtg cacaggtaga ctgagcatca tcagcatcct gttgtgaaac accgttctct
    51661 ttcaccagtc gggcataacg ctgcgctttc tggcaatcgg cttttaccag tgcctgtgct
    51721 tgttgtaatg ctgcccgagc ttcattccat gcagcctgat aactggccgc atcaatctgg
    51781 tagagcggct gtccagcctt gaccagatca ccttccttaa ataagcgttt ctggataatt
    51841 cccccaacct gcggacgtac ttcggcactg aacgcagcac tggtgcgtcc ggttaattca
    51901 gtgactaccg agaccggttg agatttgact gtaacaacgc cgacttctgt ttctgacggg
    51961 gatgaattgt tactttgcgt attatcacaa ccagataaga gcagcattgc cactacagat
    52021 gttgctatat acttcattat tttctccagg gtgagaaata ccctcctcat taaaattaat
    52081 gaggatatgt cagggatatt cagttataac gaacccgcat cgcgaaatga atttcgcaat
    52141 gaaataaggg ggattaatat cccccgctat tggttaattc tgttcttatt tatttatcaa
    52201 catactaaca atatcctgat atatttcctg cagatgttgt ggatcgcttt tttgcggtgt
    52261 taaacgacga taaaccgtac cttccatcat ggtagcaaac acttcaacgc agcaacgaat
    52321 gtgctcgtcg ctaagatggg gatgcattct tttcatatgt gcacaaccgt tagcgaacat
    52381 tctggcgtca gcttcttcaa gcattgccat aacccgcggg ttgcgtgtgg cttctgcagc
    52441 cacttccagc atcagcgcgt cgtcgtcctc atttaaggat ttacgcaagg caaggacttc
    52501 cgggaagtga tcggtacgcg cgtcaatgtc catttgggca atacgaaagt cgatgatacg
    52561 gcgaaccatc tcttcaataa tggcgtcttt gttggcgaag taacgatata tctgacccac
    52621 gctaagctga gcttcgctgg caatttgcga catactggcg gcatgaaacc cgcagcgacg
    52681 aaaacaccga cgggcggcgt taatgatttc atcctgacgc tgtttgtggc gtatctccag
    52741 cttgctggtc atttctgtct cctggcctat cacttggtga gaatgatcgt tctcattttt
    52801 gctgattata cgtttctcca aagtaatgga aagtattttt atcttattca tggtgcatat
    52861 tctgataatg aagagattgt ggagattctg tgaagatagt tgacaatatt aagttggggt
    52921 caattcatca gaaaggctgc tacttgttgt cgccagttct gttttcatca tcaaacttat
    52981 cggagctaat acgaatagtt tcatagtcag attctataag atattttccc aacctacccc
    53041 ttgcaggcgt caagagtaga taacgcaaaa tgctctgtac cgcagcttct ctccggtaca
    53101 gaaagcgcaa ctatagccac ccacagccag gttgattata ataaccgttt atctgttcgt
    53161 actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg
    53221 attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa
    53281 ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc
    53341 acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttcc
    53401 aatatcgttc tgcaatgcga tgtggccgaa gatgccagca tcgacaccat gttcgctgaa
    53461 ctggggaaag tttggccgaa gtttgacggt ttcgtacact ctattggttt tgcacctggc
    53521 gatcagctgg atggtgacta tgttaacgcc gttactcgtg aaggcttcaa aattgcccac
    53581 gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg
    53641 ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac
    53701 gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg
    53761 ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac tctggcggcc
    53821 tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc
    53881 cgtaccgtta ctattgaaga tgtgggtaac tctgcggcat tcctgtgctc cgatctctct
    53941 gccggtatct ccggtgaagt ggtccacgtt gacggcggtt tcagcattgc tgcaatgaac
    54001 gaactcgaac tgaaataatc gttctgttgg taaagatggg cggcgttctg ccgcccgtta
    54061 tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct
    54121 tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa cgccacatca
    54181 ccggcaaaag ccactggtat catgaaacgc aatccagtac tacggagtat gacgttctgc
    54241 ctctggtccc ggaagccgca aaggtcagcg atccctttct actcgacgtg atccttgaaa
    54301 aagaaacgct ggcccccttc ctttcatggc tggaccctgc gcgtgttctt gcagtggagt
    54361 tgttccctga ccagcttacc gtgacccgtt cacagacctt caccgcttat gaacgcttgt
    54421 cgacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac tactattcgg
    54481 cgcgacttac gccgctcccc gggcctgatt ccaccaggga aagtaatcat cggttggcac
    54541 aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc
    54601 gccagcatct gaatgacgtc ggtcttactg cctgggactg tgtgatcatt aaccaaatca
    54661 ttggttttat tggctttcag gcgcggacaa ttgcgacatt tcaggcttat ctcgggcatc
    54721 cggtacgctg gttacccggg ctggagatac aaaactacgc cgacgcgtca ctgtttgctg
    54781 atgaatcatt acgctggcga agcagctatg aggtggaaaa actacctgaa gaacacacaa
    54841 aaagttcaac tgcagaactt tgccaactgg ccgaaatact ctctctccac cctatttcac
    54901 tttcccttct cgaaaggttg ttaaacagca cacgggttaa tacacagccg gataatcagc
    54961 ttgcggcgtt gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc gcctgtatgg
    55021 attcatcaaa tgaatataaa aaaatcagca cccttctgcg caagggcgaa aatgaaatta
    55081 accaatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata caatggctga
    55141 cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa cacgaaaaat
    55201 catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg ataaatcgct
    55261 taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac
    55321 tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa cccgctgcta
    55381 gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc
    55441 acagaaaaag gctttggctt cctggaagtc gacgcgcaaa aaagttattt cattccgccg
    55501 ccacagatga aaaaagtcat gcatggcgac cgaattatcg cggtgatcca cagtgaaaaa
    55561 gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg tttcgtgggt
    55621 aaagttcagg gcaaaaatga ccgtctggcc atcgttcctg atcatccact tttaaaagac
    55681 gccattcctt gccgcgcagc ccgtggtctg aaccacgagt ttaaagaagg cgactgggct
    55741 gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc agaactgaca
    55801 caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct tgcgcgccat
    55861 aatctggaaa aagaagcgcc agacggcgtc gctaccgaaa tgctcgatga aggtctggtt
    55921 cgtgaagatc tgaccgcgct ggattttgtc accatcgaca gtgccagcac agaagatatg
    55981 gatgacgccc ttttcgctaa ggcgttgccg gatgacaaac ttcagctgat tgtggcgatt
    56041 gccgatccaa ccgcgtggat tgctgaaggc agcaaactgg acaaagccgc gaaaattcgc
    56101 gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac
    56161 gatctctgct cactgcgcgc caatgaagtc cgcccagtac tggcatgccg catgacgctc
    56221 tccgctgatg gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa
    56281 gcgaagctgg tgtatgacca ggtttctgac tggctggaaa ataccggtga ctggcagcct
    56341 gaaagtgaag caattgccga acaagtccgt ttgctggcgc aaatttgcca acgacgcggc
    56401 gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg ctttattctc
    56461 ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc
    56521 gtcgaagaag cgatgattgc cgctaacatt tgtgcggctc gcgtactgcg cgataagctc
    56581 ggttttggta tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgctggca
    56641 gcgttgctga aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac gctggacggt
    56701 ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga cagccgcatt
    56761 cgtcgcttcc agtcatttgc tgaaattagc actgaacccg gtcctcactt tggcctcggt
    56821 ctggaagcat acgccacctg gacttcgccg atccgtaaat atggcgacat gatcaaccac
    56881 cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccacagga tgaaatcact
    56941 gtgcaaatgg ccgagcgtcg ccgtctcaac cggatggcag aacgtgatgt tggtgactgg
    57001 ttatacgcac gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc agcggaaatt
    57061 gtcgatatca gccgtggcgg catgcgtgtt cgtttggttg ataacggcgc tatcgccttt
    57121 attcctgcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca ggaaaacggc
    57181 accgtacaaa ttaaaggtga aacggtttat aaagtaactg acgttattga cgtcaccatt
    57241 gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta atctcctttc
    57301 acggcccatt ccttatgaat gggccgttta tttccccgct ctaccttcat catatcccgg
    57361 cagtttttta actgactttc gtttgaaaac tggcgttttt ccaaaactag attacaaata
    57421 aatatataaa aaaaacaatg tgtttagccg ccgttaattt catgtaagtc atgttgttcc
    57481 ttttatttaa ccgcggactc cgctgttaac cggaggatat gcatcatgaa aaccgttagg
    57541 gagtccacaa cgttgtacaa ctttctcgga tcgcacaatc catactggcg gttgacggaa
    57601 agcagcgatg ttttgcgctt ttctaccacc gaaaccacag aacctgaacg tacgttgcag
    57661 ttatctgccg aacaggctgc tcgcatcagg gaaatgacgg tcatcacctc cagcctgatg
    57721 atgagtctga ccgtcgatga gagcgatctt tctgtgcatc tggtaggacg aaaaatcaat
    57781 aaacgggaat gggctggcaa cgcgtctgca tggcatgaca caccggcagt tgctcgtgat
    57841 ttatcacacg ggctttcctt tgctgagcag gtagtttctg aagcacattc cgcaatagtg
    57901 attctcgaca gccgggggaa tatccaacgc ttcaatcggt tatgtgaaga ttacacaggg
    57961 ttgaacgaac acgacgtcat tggccaaagc gtgtttaaac tgtttatgag ccgtcgtgaa
    58021 gctgcggcat ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc atatgaagtc
    58081 gaactgtgga taccaacacg taaaggccag cggctgtttc tgtttcgcaa taaatttgtc
    58141 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggcaccga cattaccgaa
    58201 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    58261 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcatgc agataacaat
    58321 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    58381 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    58441 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaacacc
    58501 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg gctccccttt
    58561 cgcattggtt taattgaagt ttataccagc tgttcagtag gtattgcact ctctcccgaa
    58621 catggttcgg acagcgcggc tattattcgt cacgccgaca cagcaatgta cacagcgaag
    58681 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg agtatttgaa
    58741 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    58801 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    58861 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc agaagagtca
    58921 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcgaag
    58981 tggcgggata aaggcattaa cctgcgagtg gcggtaaaca tttctgcacg tcagctcgcc
    59041 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    59101 cctattgatg ttgaattgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    59161 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    59221 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    59281 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggttcgggc aatcgtcgct
    59341 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    59401 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    59461 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    59521 tatatatact tagcgctata ttccgtcatt atatatcgta ttcccggatg ataattagcc
    59581 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccccctttt
    59641 gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga acgtattgat
    59701 attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa tctcgagata
    59761 cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc gaaggcaccg
    59821 tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt
    59881 atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc cgacaacaaa
    59941 ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg gcaaaagcca
    60001 ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa ctgagttacc
    60061 tccgccgtgc acatggcttt gcagtttcgc ttgatagtga tgacgtcgaa acccgtatga
    60121 tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcgctg gttcaaccgg
    60181 gcgaaaccat ctttatcgaa aatggcagca gcaatgccct gctcgcccgg actctggggg
    60241 agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat ttgctgaaag
    60301 acgcgccttg tgaagttatt ttgctcggtg gcgtgtacca gaaaaaaagc gaaagtatgg
    60361 ttggcccgtt gacacgccag tgcatccaac aggtacattt cagcaaagca tttattggta
    60421 ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt accgatgtgg
    60481 tgaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg
    60541 gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt
    60601 cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgtcg
    60661 ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa tcatccgctc
    60721 taagatgatt cctggttgat aattaagact atttacctgt tattaacact ctcaagatat
    60781 aaaattatta tcagcgatat aacaggaagt cattatcacc tgcgtgatat aaccctgcgc
    60841 gcgagcggat ttcacggaat aatttcacca ggcttattct tagctattat agttatagag
    60901 agcttacttc cgtgaatcat aaattcagga gagagtatta tgtttgtaac gagcaaaaaa
    60961 atgaccgcgg ctgttctggc aattaccctg gcaatgtctc tgagtgcctg ttctaactgg
    61021 tctaaacggg accgcaacac cgcaatcggc gcgggtgcag gggcattagg cggtgcagta
    61081 ctgaccgatg gcagtacgtt gggtacatta ggtggcgcag ctgtcggtgg tgttattggt
    61141 caccaggtcg gtaaataatc gtaaataaat gtcgattatc gactcgcaat tataatacgc
    61201 ttgattctgt tcagtacgaa ataactgaat aatacacaca gtaaaatatt cgaaagccac
    61261 ggatatatcc gtggcttttt atgttaaccg cctgcgagtt ttaccttcat ccctttcgct
    61321 tcaagtagtg attttaataa atcacgctta tcgccctgga tttcaataac tccatcttta
    61381 actgctccgc cgcagccgca ttttttcttc agttccgctg caagttttgt cagttcggca
    61441 tcatcgatat cgataccggt aatcaggcaa acgcccttac ctttacgtcc actggtctga
    61501 cgctgaatac gcaccacacc gtcgccttta gggcgtactg ggggcgcttt tggctcatca
    61561 atacgtccgg tttctgttga gtagaccaga cggctgttgg aatcactcat catgcactcc
    61621 gctgtaaaga ggcgttgatc gctttcagcg tctgcgctgg atctaccgat tgcgttaccg
    61681 ggcgaccaat caccatataa tcaacaccag ccgccaacgc ctgttctggc gtcataatgc
    61741 ggcgctggtc accagcttca ctcccctgcg gacgaatgcc cggtgtaacc agtttgaact
    61801 cctgaccgaa tacctgttta aagcgcacag cttcctgagc agaacacacc acaccatcaa
    61861 ggccacattt ttgcgtcagt gccgccagac gttctgcata atctgcaggt gacagtgtca
    61921 cgccaagatc ggccaggtcg ctggcttcca tgctggtcaa cactgtcaca gcaatcaaaa
    61981 gcggtgcatc tttgccatac ggaaccagtg cctcacgcgc tgcggtcatc atacgcgccc
    62041 caccagaggc atgaacattc accatccaca cgcctaagtc agctgcagca gcgacagcgt
    62101 gcgctgcagt gttggggata tcgtggaatt tcaggtcaag aaagatatca aaaccacgct
    62161 gttgaagttc gcgcacaaac tgtggcccaa acaatgtaaa catctctttg ccgaccttca
    62221 gacgacaatc gcgtgggtcg atcttgtcga caaaggccag cgcggcatca cgattatgat
    62281 aatcaagggc aacaaccaca ggagaattcg taacagcgcg ggaagaagat gaagcagtta
    62341 acgtcatgac cagaccttct tgatgatggg caccggaagt ggcgcgaaac aggtaaacgg
    62401 cgagcattct acccgtggaa ataaaaaatt aacagttgcg atttctcccc tggcaggctt
    62461 tttctgcgcc gtcgttgccg gagcacaatg gaacatcaat aacaagtatg ttgtaactaa
    62521 agtcggattt ttttaaaaat tacaggccat caagaccgcg aatcggttta attgttgacc
    62581 aggcccgaca agacggacaa tgccagtaga gggtgtatgc ggtaaaacca catttctggc
    62641 agcgataacg aggcttacta cgtacctttt cgccaaccat gtcacgcagc accatcaggc
    62701 tctctttggc acgcccttct tccgcttcat ttaagtggta atccattaac ttatggaaca
    62761 cacgcatggt cggatgacgc tgaagctggc gcgtaatata gacctgtgca gcctcactac
    62821 cgtcgcgcgc ttcgatgata tccgcaagca tcaattcagc atcggcaccg gtgttctctt
    62881 ccaccgcgcg ctgcaggaat tctgcccatt cggcagtttt acccaactgc tgatagcagg
    62941 tttgcaacat ttccagcgtt tcgctaacca gttctctgtc ctgggatatg acacgttgca
    63001 gactttcgac ggctttggcg tattctcctt tcgccataaa cacgcgtccc atcatgatgg
    63061 atacgcgggc gctgttttta tctgccgccg cccctttttt tagcaaggtc atggcacgat
    63121 cgagatcgtc gctggccata tgctgcaggg ctaactcaca gtagaaatgg gcaatttcga
    63181 cgcgctgttt atctttaccc agcttcacca ggcgttcggc aacatcaatt gctttctgcc
    63241 actcactggt agcctggtag atttgtagca actgttgcag cgcgccaatg cggaagtcag
    63301 tttcatcggt cagctgattg aacatgtctt ccgcgcggtc atataacccg gcggccatgt
    63361 aatcacgccc cagttgttga atcgccaaca gacgctgttc ataggtcagc gaggcgcttt
    63421 ccattagggt ctgatggatg cgaatagcgc gatcaacttc gccacgcgaa cggaacaggt
    63481 ttccgagcgt aaggtgggct tcaacggtgc ctgtatcctc tttaagcata tcgagaaaca
    63541 ggtctaccgc tttatcctgt tgattactaa gcaggaagtt aacccccgct acgtaatcac
    63601 gcgacaagcg gttggcttca tcttgcttgt tttgttgcgc acttctgcgg cccatatacc
    63661 agccataggc agcggctaca ggcaaaagca gaaacaacaa ctccagcata gaaagttatt
    63721 ccttcgccgc tgacgagtgc ggcactacag ccacgtcagt cgcgggtgaa agctggtttt
    63781 ccagtcgctt tattttacgt tcagcgcgcg ccagggaaac acgaactcgc agccagaaca
    63841 ggccacaaat caaccaaccg atagcaaacc ccgcagcaaa caataccgcc agcaatgtgg
    63901 aaatacggta ctccccttgc gctaacagat aattaaacgt cacctgttga tcgttctgcg
    63961 cacccaacgt gaccgaaatc acgaagatcg ctaacaccag taagaaaatg agtaaatatt
    64021 tcacattact tcccgttatg tggttagagc gaataaatta tggtcaactt accgcaatag
    64081 ccctttaaag taccattttc gcgccgtctg cgaaatggca tccacgcgca ttgttgatga
    64141 tttaagggct aaattcggaa aatcaaccag cgttaacttt cttgttctcg ttgcgctatt
    64201 tcgcgatttt cttccgcagg tggtgttaat ggcccacaaa ttcgttgcgc aagccacgtt
    64261 gccaccgcca ccagcgccca cgaaatcaac gtagctacta ccagatcgcg tggccaatgc
    64321 atcccgagca gcaggcggct tcccatgact ccagttgccc agaccaacaa gatagcaatg
    64381 gttaacgttc gccgacgcgg ccacagcaaa ccaacggcca gtagtgccca actggcagca
    64441 aacatcgtgt gaccggaagg aaaggcaaac cccgtctctt tctgccagtg tgaacgcaaa
    64501 tattgtggga tatttttctc ttcagccaac tgttctttca ctagatttcc gcgttctgct
    64561 cgctttaaag tgtagaactc atcaaccgga atatgatgtg ttttttccaa ccagataaca
    64621 aaaggtcgtg gttcctggac tttgtctttg atccaggatt taacgccttg tccaacaagg
    64681 attgcggccg ccagaatggc aaataataca aaggcagcct taatgcgaaa acgcagacac
    64741 cagagaaacc agccgaataa aatcaaatgt gtaatgacgc cccagggctg ggtgacagtt
    64801 tcagtaaccc aaaaagccgc ttttagtagc caactttgtt ctccaggttg ccaacgccag
    64861 ccagaaatcc atacggctac tggcataaca agcaatagtg cagctcccac tgcggtacgt
    64921 ctggcaatcg aacgcatggc ctctcctttt tgataagtcc cacaatcata actgaaaatg
    64981 ccagttccag gaaaaattga cagatttgtg ccattccgtg aacgatcgac gcgtcgtgat
    65041 taggtgaacc ccttctcgtt atggcaaaat aagccaatac agaaccagca ttatctggag
    65101 aatttcatgc agcttaaacg tgtggcagaa gccaaactgc caaccccatg gggcgatttc
    65161 ctgatggtgg gatttgaaga actggcaacc ggacacgatc atgtcgcgct agtctatggc
    65221 gatatttccg ggcatacccc ggtacttgcg cgcgtccatt ccgaatgtct gaccggtgac
    65281 gccctgttca gcttgcgctg cgattgtggc ttccagctcg aagcggcatt gacgcaaatt
    65341 gccgaggaag gccgtggtat tttgctgtat caccgtcagg aaggtcgtaa cattggtctg
    65401 ctgaataaaa tccgcgctta cgcactgcag gatcaaggtt acgatactgt agaggctaac
    65461 caccagttag gcttcgccgc tgatgagcgc gacttcactc tttgcgctga tatgttcaaa
    65521 ctccttggcg tcaatgaagt ccgcttgtta accaataacc cgaaaaaagt cgaaattctg
    65581 accgaagcgg ggattaatat tgttgaacgc gtaccattga ttgtaggtcg taaccccaat
    65641 aacgaacatt atctcgatac caaagccgag aaaatgggcc atttgctgaa caaataaccc
    65701 tcttgcattg tgtaattaat ttgcttgccg gaagcaaaat aaccggcaag caaatacttg
    65761 ttacttcaac atattacgaa tgacataatg caaaatgccg tcgttctggt agtaggtcaa
    65821 ctccgtcgcg gtgtcgatac gacaacggca gggtacgact tcctggctac catccgcgcg
    65881 cgtaagcgtc accggaaccg tcgcgccggg ttgtagattt tgcagatcgc caatatcaat
    65941 cttctcttcc ccggttagcc ctaacgtttt acgcgttacg ccttgcggaa attccagcgg
    66001 caggatgccc atgccaatta aattcgaacg gtgaattcgt tcaaacgatt cggcaatcac
    66061 cacacgaatt ccaagcagac gcggaccttt tgccgcccag tcacggcttg agcctgatcc
    66121 atactctttc ccggcaatta ccgccagcgg cgtttgctcc tgcttatagc gcatcgcagc
    66181 atcataaata gagactacgt cgctgtcagg taaatgccgc gtcatccccc cttcaacgcc
    66241 aggcaccatt tcattacgga tgcgaatatt ggcgaaggtg ccgcgcatca tcacttcatg
    66301 gttaccacgc cgcgaaccgt aggagttaaa gtcttttcgc tcaacacccc gaccttgtag
    66361 atatcgaccc gccgggctgt cgggcttaat actgcccgcc ggagagatat ggtcagtggt
    66421 gactgaatcc cccagcattg cgaggatccg cgcaccgtga atatcttcca caggtgctgg
    66481 tgttgcctgc atttcatcaa agaaaggcga taaacgaata taggttgaat cctcctgcca
    66541 tccgtaggta tcggatcgtg cgacgttaat ttctttccac tctgcagtgc cttcaaaaac
    66601 ttctgcgtac tctttgcgga acatttctgt ggagacttgt tctaccgcac gggcaatttc
    66661 ttgtgccgat ggccagatat ctttcagata aaccggatcg cctttgcgat catggccgat
    66721 aggctcagaa gccaggttga tattcatatt tcccgccagc gcataggcaa ccaccagcgg
    66781 cggcgaggcc agccagttag ttttaaccag cggatggata cggccttcaa agttacggtt
    66841 gccggacagc accgcaccga cggttaaatc gccttttttg attgccgttt cgataggatc
    66901 gggcagcggc ccagagttac caatacaggt ggtacaaccg tatcccacaa ggttaaaccc
    66961 cagttcgtcg agatacggtg tcagtttcgc ttttgccaga tagtcagaaa caactttcga
    67021 gcctggtgcc agcgacgctt tgacccacgg ttgccgcttg aggcccagag ttacggcttt
    67081 tttcgccagc aagcctgcag ccatcagcac acttgggtta gaggtgttgg tgcacgaggt
    67141 tatcgcagca atgaccacag cgccatcagg taactgatac tgatgtccgt tcataacata
    67201 atcgaccggc tggcgatctt tatgcgtggc attcacttcc agttcgttac tggcggcaaa
    67261 tgcttttggt acatcgggca gtgcaacgcg atcctgtggg cgttttggcc ctgccaggct
    67321 cgcttcaacg tcattcatat ccagttctaa cgtactggta aaaatcggtt catcgcccgg
    67381 gttacgccac atgccctgcg ctttggcgta tttttcgacc aactcgacct gatcttcact
    67441 gcgcccgctt aaacgcatgt aatcgagggt gacagcatcg attgggaaga agccacaggt
    67501 ggcaccatat tctggcgaca tattggcaat ggtggcgcga tccgccaacg gtagtgaatc
    67561 cagaccatca ccataaaatt cgacgaattt cccgaccacg ccatgtttgc gcagcatttg
    67621 ggtaacagtg agaaccaggt ctgtggcggt aataccttca cgtaattttc cggtaagttt
    67681 gaagcccact acgtccggga taagcatgga aaccggctgg cctaacattg cggcttctgc
    67741 ttcgatccca ccaacgcccc accccagcac gccaaggccg ttgatcatgg tggtgtgcga
    67801 gtcagtacca acgagtgtat ccggataagc aatccattca ccgtcctgca attcactcca
    67861 cactgctttg ccgagatatt cgaggttaac ctgatggcaa atgcctgtgc ctggcggcac
    67921 gacgctaaac cgactgaacg cctgctttcc ccatttcagg aacacataac gttcgtggtt
    67981 gcgctccatt tccaggcgta cgttttcttc aaatgcctca tcatcaccaa aacgatcgac
    68041 agtcaccgag tggtcaatga ccaggtcgac cggtgagagc ggattaacct ttgcagtatc
    68101 gccgccgagg cgtttaaccg cttcgcgcat tgccgccaga tcaacaacgg caggtacgcc
    68161 ggtaaagtcc tgcatcagca cccttgccgg gcggtaggca atttcacggt cagcatgggc
    68221 atttttcagc catcccgcca gcgcgtggat atcctcttcg gtaaccgagt taccatcctg
    68281 ccagcgcagc aggttttcga gcaaaacttt gagtgacttg ggtagacggg tgatatcgcc
    68341 cagtgatttg gcagcaagcg gcaggctgta gtagtggtaa gttttatctt tggcctgcaa
    68401 cgtgtcctta ctggcttctc gtagggttga cgacatagct cctccttaat gacagggttg
    68461 cgaagccctg attctcttca gggtgaatat taaagataac acaaacatcg cgtaacgatt
    68521 tgataacaac ccaaattgat aaaagaggag aaaccttgtg gcaaagcaaa tgacaacccc
    68581 gccgcagcgg ggtcaaggat cagtgagtaa tgatccagag gagttcggcc cagaagagca
    68641 tcgacagaga aaatgcaccc atccaggacc aatatttcat atttgtgttg atcataggct
    68701 acacccttaa cggttattac tttgtgttga ggtaactcaa cctgttattg ctgcggtgta
    68761 ttaaaagccc tttaattcat taaaaatggg taataagaat gatgataatt attgtttcgg
    68821 ctgatcatct tcagcaaaca gttctataaa ggcttgctgc tgcgatgtta gttgccagtc
    68881 gtctggaata acgacggaac attcccgctt cgtttctggt tcattgtcac tatcctggca
    68941 taatggtgac gactccagtt gctcctgacc aaccatagtt atccccacac tttccaaatc
    69001 agcagtgcga caaccaccca gaaaagtgca gaaccaagaa aaaccgccag ccaggcttta
    69061 cgttttaatt caggatcgcg gcggggttcc tgatttccag aaggcattgc taacctcata
    69121 taatcgccat cacttatcag caagacgttg aacgattggt gtaattaatc atacgatgag
    69181 acaaatccta aagaaacttt agtcaaaaat ccagcgaatt tacgaatcgt ctccagtgaa
    69241 taattcaatc ttttgacctg aaatagatat ttgaaaactg gaatttgcga ggcgggtaat
    69301 tagacactac tttacaggta atttacccta taaatatgag ggataaaaat attgtgcacg
    69361 atatacaaat aagtaacaaa agaaaatata tttcggcacc ttcgctacaa aaaaggtgcc
    69421 gaaaataacg caagaaatta tttttccggc agttttatat ctttaaacat gacctcaatt
    69481 tcttcattgg agcgcaatgc gacagccgca tcaacgacat cacgcgttaa atgcggtgca
    69541 aaacgctgaa tgaaatcata catataactg cgcaaaaaag tactacggcg aaaaccaatt
    69601 ttggttgtac tgtggctgaa gatatcgtga gcatcaacgc gcacaaggtc gggatcggcg
    69661 accggatcca ccgccatgct ggcaatgacc cctaccccca gccctaaccg gacgtaagtt
    69721 ttaatgacgt cagcatccgt tgccgtgaag acgatacgcg gcgttaaccc tgcgcggtta
    69781 aacgcagtat ccagttctga acgtccggta aagccgaagg tatatgtcac caacggatat
    69841 tgcgccagtt cttcaatggt aatggctttt ttgcctgcca gcgggtgatc cggagtgact
    69901 acaatagccc gattccagtg gtagcacggt aacatcacta aatcttcata cagatgcagc
    69961 gcttctgtgg cgatagcgaa atctgcatta cctttagaga cggcatcagc aatttgtgtc
    70021 ggcgagccct ggtgcatatg caaagaaacg cggggataac gctcaataaa gcctttgatg
    70081 acgtttggta acgcgtagcg tgcctgggta tgcgtggtgg cgatatacag cgagccttta
    70141 tccggccagg tgtgctcacc ggcaaccgat tttatggcat caactttcga caggacttcg
    70201 cgagcgatac gaattatttc ttgtcctgct ggcgttacct gcgtcaggtg cttgccgctt
    70261 cgggaaaaaa tttgaatgcc tagctcgtct tccagcattc tgacttgttt actgatcccg
    70321 ggttgtgatg tgtaaagtcc ttccgctgtt gatgagacat tcaggttatg attgaccacc
    70381 tcaacaatat agcgaagttg ttgtaatttc atgttaaacc atccacttag acgccatcag
    70441 aacatcgttt tttcgtcaca cctggcgggt gactaatacg atttaataat aaaaaaggtg
    70501 ctaactataa ccactatatc atttatagcc ttagtgtata ggtgtgaaga aaaaataata
    70561 tgcaataaaa aagggccgct ttcgcgaccc tttgtttata aaaacctgac agaattaaag
    70621 gttatttttt cccttcaacc catttgccat caacataaaa tgctgaccag ccagtcgcct
    70681 ttccgtcttt ttccgaagag acatattgat gtttggtttt acggctaaag cgaaccatag
    70741 tcttattacc ttccggatcc tgctgcggcg catcggccag ataacgcagt ttttccggca
    70801 gacgatcgcg gaagcgatac agctcttcca ccagcggcgc acgcgtttca cgcgatttcg
    70861 ggaaggtatt ggccgccagg aacacaccgg cagcaccgtc acgcagcacg aaataggcat
    70921 ctgatttttc gcacggcagc tccggtaatg gtaccggatc ttctttcggt ggagccactt
    70981 cgccgttacg taaaatctta cgcgtgtttt tacactcttc gttggtgcag gccatatatt
    71041 taccgaaacg ccccattttc aggtgcattt cagaaccaca tttttcacac tcaacgatcg
    71101 ggccgtcata acctttaatg cggaattcgc cctcttcgat ctcgtaaccg tcgcaggttg
    71161 ggttattacc acagacatgc aacttacgtt tcggatcgat gagatagctg tccatcgccg
    71221 tgccgcattt cgggcaacga cgttttgcgc gcagcgcgtt ggtttcagcg tcttcgcctt
    71281 ccagcacgtt cagcacttcg ttttccggca ccaggttaat ggttgttttg caacgctctt
    71341 tcggcggcag cgcatagcca gaacagccaa ggaatacccc ggtgctcgct gtgcgaatcc
    71401 ccattttgcg accacaggtc gggcagtcga tgctggtcag aaccatctgg ttcggacgca
    71461 tacccccctc ttccggatct ttttcagctt tatctaactg ctgagtgaaa tccgagaaga
    71521 agtgatccag tacagctttc cactctgctt cgtgatttgc cacctggtcg aggctgtttt
    71581 ccatctgcgc ggtgaagtcg tagttcatta actcgcggaa attctcttcc aggcgatcgg
    71641 tgacgatttc gcccattttt tccgcataga aacgacggtt ttctacacgc acatagccac
    71701 gatcctgaat ggtcgaaatg atcgacgcat aggtagacgg acgaccgata ccgcgttttt
    71761 ccagttcttt aaccagcgat gcttcactga aacgggctgg cggcttggta aagtgctggg
    71821 ctggtgtcag ttcaacgagc gtcagagcat cgcctttatc gactgccggt aagatacgat
    71881 cttcatcgcc tttacgcagt gcaggcatca cttttgtcca gccatcaaag cgcaaaatac
    71941 gaccgcgtgc tttcaggcgg aaatcgcccg caccaacggt cagcgtcgtg gagtcatatt
    72001 tcgctggggt catctggcag gcaacgaact gacgccagat taactggtac agtttctgcg
    72061 catctgcttc catatccttc agcgattccg ccatcacatt gacgtcagaa ggacgaatcg
    72121 cttcgtgcgc ttcctgtgag ttttctttgc tggcgtactg attcggactt tccggcagat
    72181 atttcttacc aaaattatcg ctgatataac cgcgaaccat atttaccgcg tcctgactca
    72241 ggttagtgga gtcggtacgc atgtaagtga tatagcctgc ttcatacaaa cgctgcgcca
    72301 tcatcatggt ttttttcacg ccaaatccaa gacgggtgct ggcagcttgt tgcagcgtag
    72361 aggtaataaa aggagcgcca ggtttactgg ttgtcggttt gtcttcacgt tccagcacgc
    72421 tgtagcgcgc tttttccagc agacttaccg cagcctgagt ttgttctttg ttgaccggac
    72481 ggaacggttt gtcgttctga tgagtcacct gcaacgccaa cgcttcacca gatggcgtgg
    72541 tcgtgctggc atcgacttcc cagaactctt ccggcacgaa cgctttaatt tcacgctcac
    72601 gctcgaccac caggcgaacc gccaccgact gcacacgacc ggcagacaga ccacgagcga
    72661 tctttttcca tagcagcggc gaaaccatat accccaccac gcggtccata aagcgacgcg
    72721 cctgctgggc attaacacga tcaatattca gctcacccgg tttgttaaac gcctggcgga
    72781 tcgcgttttt agtaatttcg ttaaacacca ctcggctata gcgcgcatca tcaccaccaa
    72841 tcacttcccg caggtgccat gcaatggctt ccccttcgcg gtcaaggtcg gttgcgagat
    72901 agatgtggtc ggctttttca gccagttgtt tcagttcaga gacgaccttc tctttaccag
    72961 gcaacacttc atagtgcgcc tcccaattgt gccacgggtc aacccccata cggttgacga
    73021 gagcgccacg ttcatccttt ttaggctttt tagccgtctt ggtggaggta gagtcggcac
    73081 tctttttggc agctgagcca ctggtcggca aatcgcggat gtgaccgacg ctggatttaa
    73141 ccacgtagtc actacccaga tacttgttga tcgttttggc ttttgccggg gactcaacga
    73201 tgacaagagc tttacccata ttcaccttac ctaatttgat tcttccagga atgcgtcgca
    73261 cgttgataaa ccttccactg gcaacgagtt accgatatgg actcgctctc tgcggatatc
    73321 aaccccttgt cgtgactctg ttcacgaaaa ttcaggtgac tgagttaaca ggtcttggcc
    73381 tacaggcgct atagcacgac ggaattttgg tcgaatgtca agcaaatcta ttgccagatt
    73441 gacgaaagcg tcacactgta cctgataaaa tccgatacgc aactttatta gcatgcaaaa
    73501 tccaaagttg ccagtaaggc gctcttgcca ccagcgtggt atatttgtct gcaagccatg
    73561 cctggcgtag actaatgtcc ttgtttaagg agtaaataat gaacaaagag acacaaccca
    73621 tcgaccgcga aacgctgttg aaagaggcca acaaaatcat tcgtgagcat gaggatacgc
    73681 tcgcagggat tgaagccacc ggcgtcacgc agcgtaatgg cgtgctggta tttaccggcg
    73741 actacttttt agatgaacaa ggattgccaa cagccaaaag caccgcggtg tttaatatgt
    73801 tcaaacacct ggcccatgtg ctttcagaaa aatatcatct ggttgattaa agcaaaacgc
    73861 gaggtcttac cctcgcgttt gtcttttaca tcaatggctt ttgcccacgc tgccaccagc
    73921 gtagcaataa tctatcggcg ctctctgccg cgctgccggt gaatcggtca atgagtcgtt
    73981 tacgctgcat atagcgtaca ttgaccactt cacggccttc catcaggcta agaataactt
    74041 catcactggt gttgatttca tcaaccaggc ctttctctac cgcctgttgt ccgtaccagt
    74101 gttcacccgt tgccacctgt tcaatatcca gagacggacg catacgcttc acaaaatctt
    74161 taaataactg atgcgtttcg ttcagctctt cgcggaattt ctcccgccct tcttcggtat
    74221 tttcacccag caaggtcagc gtgcgcttat actgcccggc ggtgtgcagt tcgatatcaa
    74281 tatctttgct tttcaggaag cggttaaagt tgggcatttg cgccaccaca ccaatggaac
    74341 ccacaatagc aaacggtgcg gaaacaattt tgtccgccac acaggccatc atgtaaccgc
    74401 cgctggcagc gactttgtct accgtaacag ttaaaggaat gtttttatca cgcagacgct
    74461 gcagctgcga agccgccaac ccgtaaccat gcaccatgcc accagggctt tccagacgta
    74521 gcacaacctg atcctgcggt ttgaatgctg cgagtacagc cgttatctct tcacgtagcg
    74581 agttcacttc atgggcgtcc atgctgcctt taaaatccag cacccagacg cggggtttac
    74641 tgtcagttgc cacctcgccc agtttggctt tcgcttttgc tgctttcgct tcttgcttat
    74701 gctttttctt ctgcgctttg tgccactgtt tttgctgatg tgagtccatc agcgccgcgg
    74761 ccagttcttc tttcatctcc ttatactgtt cgctgagatt gttgacccgt aactcgccac
    74821 gctggcgttt attacgttga gcaacattga caataatggc ggcaatcgcc gcaatcgcta
    74881 gcacaacggt aacgattttc gccaaaaaca aaccatattc agacaacaat tccacgagtc
    74941 cccaccttgg ttaaacaacg ctttggctca cagtttacaa caggcatgtt aagccgtctc
    75001 gcagagatgt tattgcagcg aaagtatgac aaagtatcca ttttgcggtg ttatcgcccc
    75061 aggctgattg aaatgccctt ctgtttcagg cataaagccc caaagtcata aagtacactg
    75121 gcagcgcgac agacaacgtc gttgaggagt agccatgcat taccagccaa aacaagattt
    75181 actcaatgat cgcattatcc tggtgacggg agccagtgat ggtattggtc gtgaagccgc
    75241 gatgacgtat gcacgctatg gtgcgacagt gattctgttg ggccgtaatg aagaaaaatt
    75301 acgtcaggta gccagccaca taaacgaaga aactgggcgt cagccacagt ggtttattct
    75361 cgatttgctg acctgcaggt ccgaagattg ccaacaactg gcacagcgca ttgccgttaa
    75421 ttatccgcgt ctggatggtg ttttgcataa tgccggattg ctcggcgatg tttgtccaat
    75481 gagcgaacaa aatccacagg tctggcagga cgtcatacag gtcaacgtta atgccacctt
    75541 tatgctcacc caggcactgc ttcctttatt actcaaatcg gacgccggtt cactggtctt
    75601 tacttcatca agcgttggac gtcagggacg agccaactgg ggcgcatatg cagcgtcgaa
    75661 atttgccacc gaagggatga tgcaggtact ggccgatgaa tatcagcagc gcctgcgcgt
    75721 caactgcatt aacccaggcg gtacgcgcac cgcaatgcgt gccagcgcct tcccgaccga
    75781 agatccacag aaacttaaaa ctcccgctga tatcatgccg ctctacctct ggctgatggg
    75841 cgatgacagc cgccgtaaaa ccggcatgac ctttgacgcc caaccgggcc gtaaaccagg
    75901 aatttcccaa tgagtgatga acgctaccaa cagcgtcagc agcgagtgaa agaaaaagta
    75961 gatgctcgtg tggcccaggc ccaggatgaa cgcggtatta tcatcgtctt taccggcaat
    76021 ggaaaaggca aaaccaccgc ggcatttggt acggcaacac gcgcagttgg tcacggaaaa
    76081 aaagtgggcg tcgtgcagtt tattaaaggc acctggccta atggcgaacg caatctgctg
    76141 gagccacatg gcgttgagtt tcaggtgatg gcaacgggct ttacctggga tacacaaaac
    76201 cgcgagtctg ataccgccgc ctgccgcgaa gtctggcaac atgcaaagcg gatgcttgct
    76261 gattcctcac tggatatggt tttgctcgat gagctgacat atatggtggc gtatgactat
    76321 ttgccactgg aagaagtgtt gcaggcgtta aatgaacgtc cacatcaaca gacggtgatt
    76381 atcacgggtc gaggctgtca tcgggatatt cttgaactgg cggatacggt gagtgagttg
    76441 cgccccatca aacatgcgtt tgatgccggt gtaaaagcgc agatagggat cgattattaa
    76501 tctgtaaaaa tgccggatat gacgtccggc atactttgat taaccgttat tattacgtcc
    76561 gccagaacga cgaccgccgc tcacctgact gtgacgtttc accgcacgac gaatctgatt
    76621 cgccttcatg cgacgacggt ctttttctac agcgacttta gagctggttt ctggcggtag
    76681 ctccaccagt tcgcgcagat agttagtctg ggcgaggtcc agctccgtcc agccaccgcg
    76741 aggcagacct tttggcagtg ggatatcacc gtaacgaaca cggatcaggc ggctcacctg
    76801 cacaccaacc gcttcccaca gacgacgaac ttcacggtta cggccttcgg tcagagtcac
    76861 gttgtaccac tggttgatcc cttcgccgcc gctgaacttg atggttttaa aagctgccgg
    76921 accatcttcc aactgcacgc cacgactcaa atcacgcagt ttcgcgtcgt caacctgacc
    76981 aaatacacgc acggcatatt cacgttcaac ttcacggctt gggtgcatta aacggtttgc
    77041 cagttcacca tcggtggtga acagcagcag accacaggta ttaacgtcca agcgacccac
    77101 ggcaatccag cgtgcgccac gcagttttgg cagacggtca aacaccgttg gacgtccttc
    77161 cggatcgtta cgggtacaca actcaccttc cggtttgtaa taggccagaa cgcgacaaat
    77221 ttgttcagcg gactcacgta ccgaaatcag gtgaccatcg atacggattt tcaatccagg
    77281 ggtaacttca acgcgatcgc cgagtttagc aattttgcca tcaacactca cacgaccggc
    77341 ttcaataata gattcgattt cacggcgaga accgtggccg gcacgcgcca gcactttctg
    77401 tagcttttcg ctcatagagc ttccttttgg tgtcgccttc ccaggcgtct gatatgcttt
    77461 tgtgcaacag tgagtcagaa cttacatctg actcactgat agtaaagtct gtttgaactc
    77521 ttcaggcctt aagtaaacga ctcgacgttt taactggcct agctttgagg cgcaacatac
    77581 tacactatct ttatggttaa agcttgtttc gattaac
//
LOCUS       DAZGBC010000005        75346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGBC010000005 DAZGBC010000000
VERSION     DAZGBC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.005"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(235..1239)
                     /gene="trpS"
                     /locus_tag="ACHK1J_000329"
     CDS             complement(235..1239)
                     /gene="trpS"
                     /locus_tag="ACHK1J_000329"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO7909668.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1232..1990)
                     /gene="gph"
                     /locus_tag="ACHK1J_000330"
     CDS             complement(1232..1990)
                     /gene="gph"
                     /locus_tag="ACHK1J_000330"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO7909669.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1983..2660)
                     /gene="rpe"
                     /locus_tag="ACHK1J_000331"
     CDS             complement(1983..2660)
                     /gene="rpe"
                     /locus_tag="ACHK1J_000331"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO7909670.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(2678..3514)
                     /gene="dam"
                     /locus_tag="ACHK1J_000332"
     CDS             complement(2678..3514)
                     /gene="dam"
                     /locus_tag="ACHK1J_000332"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7909671.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3621..4907)
                     /gene="damX"
                     /locus_tag="ACHK1J_000333"
     CDS             complement(3621..4907)
                     /gene="damX"
                     /locus_tag="ACHK1J_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO7909672.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(4999..6087)
                     /gene="aroB"
                     /locus_tag="ACHK1J_000334"
     CDS             complement(4999..6087)
                     /gene="aroB"
                     /locus_tag="ACHK1J_000334"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO7909673.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(6144..6665)
                     /gene="aroK"
                     /locus_tag="ACHK1J_000335"
     CDS             complement(6144..6665)
                     /gene="aroK"
                     /locus_tag="ACHK1J_000335"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO7909674.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(7066..8304)
                     /gene="hofQ"
                     /locus_tag="ACHK1J_000336"
     CDS             complement(7066..8304)
                     /gene="hofQ"
                     /locus_tag="ACHK1J_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO7909675.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSTKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(8216..8620)
                     /gene="hofP"
                     /locus_tag="ACHK1J_000337"
     CDS             complement(8216..8620)
                     /gene="hofP"
                     /locus_tag="ACHK1J_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO7909676.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPRWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(8610..9050)
                     /gene="hofO"
                     /locus_tag="ACHK1J_000338"
     CDS             complement(8610..9050)
                     /gene="hofO"
                     /locus_tag="ACHK1J_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO7909677.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRVSHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(9034..9573)
                     /gene="hofN"
                     /locus_tag="ACHK1J_000339"
     CDS             complement(9034..9573)
                     /gene="hofN"
                     /locus_tag="ACHK1J_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO7909678.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(9573..10352)
                     /gene="hofM"
                     /locus_tag="ACHK1J_000340"
     CDS             complement(9573..10352)
                     /gene="hofM"
                     /locus_tag="ACHK1J_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO7909679.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            10472..13024
                     /gene="mrcA"
                     /locus_tag="ACHK1J_000341"
     CDS             10472..13024
                     /gene="mrcA"
                     /locus_tag="ACHK1J_000341"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO7909680.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYYGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESVWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(13185..13745)
                     /gene="nudE"
                     /locus_tag="ACHK1J_000342"
     CDS             complement(13185..13745)
                     /gene="nudE"
                     /locus_tag="ACHK1J_000342"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO7909681.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            14065..16200
                     /gene="igaA"
                     /locus_tag="ACHK1J_000343"
     CDS             14065..16200
                     /gene="igaA"
                     /locus_tag="ACHK1J_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO7909682.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLIDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            16265..16933
                     /gene="yrfG"
                     /locus_tag="ACHK1J_000344"
     CDS             16265..16933
                     /gene="yrfG"
                     /locus_tag="ACHK1J_000344"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO7909683.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEFMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16944..17345
                     /gene="hslR"
                     /locus_tag="ACHK1J_000345"
     CDS             16944..17345
                     /gene="hslR"
                     /locus_tag="ACHK1J_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO7909684.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            17370..18248
                     /gene="hslO"
                     /locus_tag="ACHK1J_000346"
     CDS             17370..18248
                     /gene="hslO"
                     /locus_tag="ACHK1J_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO7909685.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(18311..20035)
                     /locus_tag="ACHK1J_000347"
     CDS             complement(18311..20035)
                     /locus_tag="ACHK1J_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO7909686.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYCYFYQSVWHNVLILQVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            20414..22036
                     /gene="pckA"
                     /locus_tag="ACHK1J_000348"
     CDS             20414..22036
                     /gene="pckA"
                     /locus_tag="ACHK1J_000348"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO7909687.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(22112..23464)
                     /gene="envZ"
                     /locus_tag="ACHK1J_000349"
     CDS             complement(22112..23464)
                     /gene="envZ"
                     /locus_tag="ACHK1J_000349"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO7909688.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(23461..24180)
                     /gene="ompR"
                     /locus_tag="ACHK1J_000350"
     CDS             complement(23461..24180)
                     /gene="ompR"
                     /locus_tag="ACHK1J_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO7909689.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            24408..24884
                     /gene="greB"
                     /locus_tag="ACHK1J_000351"
     CDS             24408..24884
                     /gene="greB"
                     /locus_tag="ACHK1J_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO7909690.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            24981..27302
                     /locus_tag="ACHK1J_000352"
     CDS             24981..27302
                     /locus_tag="ACHK1J_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO7909691.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            27740..27967
                     /gene="feoA"
                     /locus_tag="ACHK1J_000353"
     CDS             27740..27967
                     /gene="feoA"
                     /locus_tag="ACHK1J_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO7909692.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            27984..30305
                     /gene="feoB"
                     /locus_tag="ACHK1J_000354"
     CDS             27984..30305
                     /gene="feoB"
                     /locus_tag="ACHK1J_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO7909693.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            30305..30541
                     /gene="feoC"
                     /locus_tag="ACHK1J_000355"
     CDS             30305..30541
                     /gene="feoC"
                     /locus_tag="ACHK1J_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO7909694.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISKTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            30744..31622
                     /gene="rpnA"
                     /locus_tag="ACHK1J_000356"
     CDS             30744..31622
                     /gene="rpnA"
                     /locus_tag="ACHK1J_000356"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO7909695.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(31649..32419)
                     /gene="bioH"
                     /locus_tag="ACHK1J_000357"
     CDS             complement(31649..32419)
                     /gene="bioH"
                     /locus_tag="ACHK1J_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO7909696.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSAREEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            32457..33140
                     /gene="gntX"
                     /locus_tag="ACHK1J_000358"
     CDS             32457..33140
                     /gene="gntX"
                     /locus_tag="ACHK1J_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO7909697.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            33199..33774
                     /gene="nfuA"
                     /locus_tag="ACHK1J_000359"
     CDS             33199..33774
                     /gene="nfuA"
                     /locus_tag="ACHK1J_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO7909698.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            34135..35451
                     /gene="gntT"
                     /locus_tag="ACHK1J_000360"
     CDS             34135..35451
                     /gene="gntT"
                     /locus_tag="ACHK1J_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7909699.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(35496..37580)
                     /gene="malQ"
                     /locus_tag="ACHK1J_000361"
     CDS             complement(35496..37580)
                     /gene="malQ"
                     /locus_tag="ACHK1J_000361"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO7909700.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(37590..39983)
                     /gene="malP"
                     /locus_tag="ACHK1J_000362"
     CDS             complement(37590..39983)
                     /gene="malP"
                     /locus_tag="ACHK1J_000362"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO7909701.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            40595..43300
                     /gene="malT"
                     /locus_tag="ACHK1J_000363"
     CDS             40595..43300
                     /gene="malT"
                     /locus_tag="ACHK1J_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO7909702.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(43343..44359)
                     /gene="rtcA"
                     /locus_tag="ACHK1J_000364"
     CDS             complement(43343..44359)
                     /gene="rtcA"
                     /locus_tag="ACHK1J_000364"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO7909703.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFKTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFPL
                     HEQYIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(44363..45589)
                     /gene="rtcB"
                     /locus_tag="ACHK1J_000365"
     CDS             complement(44363..45589)
                     /gene="rtcB"
                     /locus_tag="ACHK1J_000365"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO7909704.1"
                     /translation="MNYELLTTENASVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDGSDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            45776..47374
                     /gene="rtcR"
                     /locus_tag="ACHK1J_000366"
     CDS             45776..47374
                     /gene="rtcR"
                     /locus_tag="ACHK1J_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO7909705.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIVSVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(47356..48114)
                     /locus_tag="ACHK1J_000367"
     CDS             complement(47356..48114)
                     /locus_tag="ACHK1J_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO7909706.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(48131..48961)
                     /gene="glpG"
                     /locus_tag="ACHK1J_000368"
     CDS             complement(48131..48961)
                     /gene="glpG"
                     /locus_tag="ACHK1J_000368"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO7909707.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(49006..49332)
                     /gene="glpE"
                     /locus_tag="ACHK1J_000369"
     CDS             complement(49006..49332)
                     /gene="glpE"
                     /locus_tag="ACHK1J_000369"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO7909708.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            49522..51027
                     /gene="glpD"
                     /locus_tag="ACHK1J_000370"
     CDS             49522..51027
                     /gene="glpD"
                     /locus_tag="ACHK1J_000370"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO7909709.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHFARTYGSNSELLLGNAGAVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLSAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(51242..51832)
                     /locus_tag="ACHK1J_000371"
     CDS             complement(51242..51832)
                     /locus_tag="ACHK1J_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7909710.1"
                     /translation="MNKIRLYIYPLYLGFVIVPLLVWPTVVVVGITLSFLMEMLFSNS
                     FMHRRISLLELQLWLSAEYALFFCVMCCVGWQFTRRTQSELKSRLHCWLVFAPVYLWL
                     ILWNIIFYIAPAKIALLESIRNFFLLVVWLPLNFSPFWPQPWTDFIGPISAQLGFALG
                     YYCQWRSKNRCQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(51847..52611)
                     /locus_tag="ACHK1J_000372"
     CDS             complement(51847..52611)
                     /locus_tag="ACHK1J_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7909711.1"
                     /translation="MQCSPAMQALIEQIYHIFRRYPVPSRFHVCCPYCFSEEQQQALR
                     GVSLRAIPFPLLHAWGTSIGPTPQNDDEIRYLLPRLFEFIAQRQFPGIYEGCCLQRIA
                     EADNQNWRTDELQIISDFAYLYMQDWVSAKEVVELEIILEMFYRGGIKIEPLLDAIMS
                     ISGYWPTASMACLLWRNSKEYVENSDFEQSDDNKTITTQINTWARNNHPLLKERARQA
                     IENPLKQPEPMTEYQVWEDDWMIDECLCAMYDASPE"
     gene            complement(52714..54222)
                     /locus_tag="ACHK1J_000373"
     CDS             complement(52714..54222)
                     /locus_tag="ACHK1J_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7909712.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDIWAW
                     QPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSMTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIIDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(54347..56794)
                     /gene="glgP"
                     /locus_tag="ACHK1J_000374"
     CDS             complement(54347..56794)
                     /gene="glgP"
                     /locus_tag="ACHK1J_000374"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO7909713.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(56813..58246)
                     /gene="glgA"
                     /locus_tag="ACHK1J_000375"
     CDS             complement(56813..58246)
                     /gene="glgA"
                     /locus_tag="ACHK1J_000375"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO7909714.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(58246..59541)
                     /gene="glgC"
                     /locus_tag="ACHK1J_000376"
     CDS             complement(58246..59541)
                     /gene="glgC"
                     /locus_tag="ACHK1J_000376"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO7909715.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(59559..61532)
                     /gene="glgX"
                     /locus_tag="ACHK1J_000377"
     CDS             complement(59559..61532)
                     /gene="glgX"
                     /locus_tag="ACHK1J_000377"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO7909716.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDTNGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(61529..63715)
                     /gene="glgB"
                     /locus_tag="ACHK1J_000378"
     CDS             complement(61529..63715)
                     /gene="glgB"
                     /locus_tag="ACHK1J_000378"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO7909717.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(63988..65091)
                     /gene="asd"
                     /locus_tag="ACHK1J_000379"
     CDS             complement(63988..65091)
                     /gene="asd"
                     /locus_tag="ACHK1J_000379"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7909718.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            65283..65876
                     /gene="yhgN"
                     /locus_tag="ACHK1J_000380"
     CDS             65283..65876
                     /gene="yhgN"
                     /locus_tag="ACHK1J_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO7909719.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(65922..67217)
                     /locus_tag="ACHK1J_000381"
     CDS             complement(65922..67217)
                     /locus_tag="ACHK1J_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO7909720.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(67124..69220)
                     /locus_tag="ACHK1J_000382"
     CDS             complement(67124..69220)
                     /locus_tag="ACHK1J_000382"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO7909721.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(69360..70700)
                     /gene="gntU"
                     /locus_tag="ACHK1J_000383"
     CDS             complement(69360..70700)
                     /gene="gntU"
                     /locus_tag="ACHK1J_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7909722.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(70704..71231)
                     /gene="gntK"
                     /locus_tag="ACHK1J_000384"
     CDS             complement(70704..71231)
                     /gene="gntK"
                     /locus_tag="ACHK1J_000384"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO7909723.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(71370..72365)
                     /gene="gntR"
                     /locus_tag="ACHK1J_000385"
     CDS             complement(71370..72365)
                     /gene="gntR"
                     /locus_tag="ACHK1J_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO7909724.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(72589..73284)
                     /gene="yhhW"
                     /locus_tag="ACHK1J_000386"
     CDS             complement(72589..73284)
                     /gene="yhhW"
                     /locus_tag="ACHK1J_000386"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO7909725.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(73407..74444)
                     /locus_tag="ACHK1J_000387"
     CDS             complement(73407..74444)
                     /locus_tag="ACHK1J_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7909726.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            74779..75267
                     /gene="yhhY"
                     /locus_tag="ACHK1J_000388"
     CDS             74779..75267
                     /gene="yhhY"
                     /locus_tag="ACHK1J_000388"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7909727.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        18287 a        20463 c        18824 g        17772 t
ORIGIN      
        1 tatcttcatt aaaatgaaga taagtggcaa cggtgagatg tggaaacggc gaggcgcttc
       61 acattttttc ggattattgc aaatgaggat aaaaaaaccg ggtttccccg gtttcagagt
      121 gatgacgaag tgatttattc gacaatgcga gcaaagggta attcccactg gcgcttgcct
      181 gctcccctca ccctctcccc aaaggggcga ggggaccgtc cgagcgtttt gatattacgg
      241 cttcgccaca aaaccaatcg cttcgtacac cgcttttagc gtacgggaag cgtgcgcgct
      301 ggctttttct gcgccgtctt tcatcacctg ttgcaggaag gcttcatcgt tgcggaaacg
      361 gtggtaacgt tcctgcaatt cagtcagcat accggaaacg gcatcagcca cttcgccttt
      421 cagatgacca tacatcttgc cttcgaactg tttttccagt tccgggatgc tctggcccgt
      481 taccgcagaa aggatatcca gcaggttgga aacgcccgct ttgttctgca catcgtagcg
      541 aactaccggc ggctcgtcgg aatcagtgac cgcgcgtttg attttcttca ctaccgattt
      601 cggatcttcc agcaggccaa taacgttatt gcggttatcg tcagacttgg acatcttctt
      661 ggtcggctcc agcagcgaca ttacgcgcgc gccggatttc ggaataaacg gctccggcac
      721 cttaaagatc tcgccgtaca gcgcgttgaa acgctgagcg atatcgcggc tcagttccag
      781 gtgctgtttc tggtcttcac ccaccggtac cagattagtt tgatacagca ggatgtccgc
      841 agccatcagc accggatagt caaacagacc agcgttgatg ttctcggcat aacgcgcaga
      901 tttatcttta aactgcgtca tgcggctcag ttcgccgaag taggtatagc agttcagtgc
      961 ccaacctaac tgtgcatgtt ccggcacgtg ggactgaaca aaaatggtgc ttttctcagg
     1021 atcgatacca caagccagat acaaggccag cgtatccagc gtcgctttac gcagcttctg
     1081 tgcatcctgg cgcacggtga tcgcgtgttg gtcaacgata cagtaaatgc aatggtagtc
     1141 atcctgcatg tttacccact gacgcagcgc acccatgtag ttaccaatgg tcaattcacc
     1201 tgagggctgt gcgccactaa aaacgatggg cttagtcatt tttcgattcc tgattttcgc
     1261 tatgcggaag ccctaatgcg ggcagaaggt catttataga ctgataaatt acatcaggct
     1321 ggctgagatc gatagcctcg ccgtagttat atccgtaggt taagccaact gatgggcaac
     1381 ctgccgcttt tgctgcctga atatcattgc gtgagtcgcc aacaaacagc atctgttgtg
     1441 gagcaattcc catccgctca gccaccagta acagcgggtc cggatgcggt tttttgtttt
     1501 gcacatcatc accaccaatc accacgctga agtatttagc gatatctaag gcttcgagca
     1561 gcggcgcgac gaacggcgtc ggtttgttgg tgaccaggcc tagcggcagg cctttagcct
     1621 gcaacgcgcc caacgtatcg gcaacgtgcg ggaacaaaaa cgtcccctct tcggcaacct
     1681 cgccatagta gcgatcgaac agtttacgca gaatacgtac ctgttcttct gccggaatgt
     1741 cgtcatcaac gggcggttta cccattgttt tacgctgagt cgcacgttcc tgacgcgccc
     1801 aggtcaatgc gcgctccatc agaacatctg cgccgttacc aatccaggta ataacgcgtt
     1861 cttcacctgc gacgggcaac tccagcgcat acagcgccat atctaccgca gcagcaagac
     1921 caggagcact gtcgaccagc gtaccatcaa gatcaaaagc gacgccgcga atatcttcaa
     1981 acttattcat gacttacctt tgccagttca ctgcgcattt catcaatgac ttttttatag
     2041 tctggctggt cgaagattgc cgaaccggcg acgaacatat ccgcgcccgc cgcagcgatt
     2101 tcgccaatgt tgttcacctt cacgccaccg tccacttcca gacgaatgtc aaagccagac
     2161 tcgtcgatac gacgacgtac ttcgcgcagt ttatccagtg tttgcggaat gaaagactga
     2221 ccgccgaaac cagggttgac ggacatcagc aggatcacat ccagcttatc catcacgtaa
     2281 tccagatagc tcagaggtgt cgccgggtta aataccagac ccgctttaca gccattttct
     2341 ttaatcagtt gcagcgtgcg gtcaacatgc tcggaggctt ctggatgaaa ggtaatgatg
     2401 ctggcaccag cggcagcgaa atcaggcaca atgcgatcga cgggtttcac catcagatgt
     2461 acgtcgatag gggcggtaat gccatagtta cgcaaggatt tcagcaccat tggcccaatc
     2521 gtcagattgg gaacatagtg gttatccatg acgtcaaaat gcacgacatc agcgccagct
     2581 gccagggctt ttgcggtatc ttcacccagg cgggcaaaat cagccgacag aattgagggg
     2641 gcaatcaaat actgtttcat ccgcttctcc ttgagaatta ttttttcgcg ggtgaaacga
     2701 ctcctggttt gtacaaagcc agcagttcgt ccaccttttt acgtgtgccg ccgttgctgc
     2761 ttatactgcg tcgaactttg acgacatgca attttgcgcg ctgataccac tcacgcgtta
     2821 acatcgtatc gtgattggag atcagcacag gaatatggcg atcaaccaga ccttcggcga
     2881 tctccgccag atgcgcttgt tgttcaagcg taaaactgtt tgtgtgatac gccgtaaagt
     2941 tggcggtcgc agacagcggc gcataaggcg gatcgcaata gacgacggat gcatcatctg
     3001 cacgcgccat gctatcggcg taagactcac aatagaaaaa ggcattctgc gctttttcag
     3061 cgaagtgata caactctgct tccgggaaat agggtttttt gtagcggcca aacggcacat
     3121 taaactcacc gcgcagattg taacgacaca ggccgttgta accgtagcgg ttcaaatata
     3181 aaaacagtac cgcccgacgg aacggatcct ggcttttgtt gaactcttcg cggaactgat
     3241 agtaaacctc ggcgcaattt gtttcgggaa caaacagctc gcgtgcggcc tgtacgtact
     3301 catcagtacg catcttcaca atgttataga gactgatcag gtcgctattg atatcggcaa
     3361 ggatataacg agaaaagtcg gtgttgagaa acaccgaccc ggcacctaca aaaggctcaa
     3421 ccagacattc gcccttgggc aaatgccgtt taatatcatc aagcagggga tacttgcccc
     3481 ctgcccactt caaaaaagcg cgattcttct tcatgctgac taactaatta caccttctcc
     3541 ggctgtggag aaagctccga cagcatccag cgcttcaggc aactcccgca acgataaacc
     3601 attgcgggag ataaccttga ttacttcaga tcggcctgta cctgacgcag cggtttcgcc
     3661 cacgggtttt tggcctggac atctgctggc aatgtagata ccgctttttt cgcctcttct
     3721 ttcgaagcgt acacgccaga aaccaggaca taccacggct gaccattacg cgtcgtttca
     3781 tagacaacgt agtttttcag attctctttc ttcgcccaac cgttcaggtt gtcgtagtta
     3841 gaggaactgc tcagctgcag agtgtaatgg ctggacggtg ccgatttcaa cgaaccaaca
     3901 ttacctgcgg tcttccctcc agcggctggt gttgccgtgg tttgcgccgg ggatgccgtc
     3961 tgtactggag ccgtagtcgc cgtctctttt ggtgctggcg tagaagtcgc agccggtgcc
     4021 ttcgtgctcg ccacaggagc agctggttca gtacgcttcg gcgtctgtgc taccggcttc
     4081 ggttccgttt tcacggttgc ttgcggtttt ttcggttcaa tcaccgcctg ctgacgagct
     4141 ggacgcgtag tggccggacg ttcagcagtt tgcgttttcg ccgtgtcacg cgatgcattg
     4201 ccattgcgta ctggcgcgac agttgctggt tcagtcggca atgtagaatt gaccgccaca
     4261 ttgttcaact gttgctgatt ttgtggctgg gtcagcgcat tgttcaggtc accctgcact
     4321 tcaacacgtt gttgaccatc cgttgccacc ggggtttgcc cttgagtcgg cgtagaagag
     4381 atcggtggca gagaaacatc ctgctgagta ttttccgcag acgtggttcc cggcgctggc
     4441 tgcacgccat tcgcctgatc ggtcgcattg ccagcaagat caatactctt ctcgccagac
     4501 gcggtttgat cgctggaagt ggtcgagggg gcttttagcg cagaaccgat accgatgatc
     4561 aacagcagta gaaccagaat gccgacgccc atcatcatat actgacgaga agcgggttta
     4621 ctggctgctt ttttgcgctt acgcggacga cgctctacgc gctcttcatc cacggtttca
     4681 tcttcggatt cgtcaatttc ttcttcgatt tccggttcct cattgcgctc tttttgcgca
     4741 cgagtcggac ggcgatcgtc agtgtcatca agttcaatat catcaaaatt gatctgcggt
     4801 tcgccacgtt cagtacgctc agaacgttca gaagattgac gagaacgacc agtacgacga
     4861 tcgctgggat cgggtttcag ctcgtcttct ggtttgaatt catccattta acaccccact
     4921 aaaaggttaa tgcttaccac gttgcaatta acctgaagct aatagaccgc ttgataagcg
     4981 gcctgacctt tcttgttgtt acgctgattg acaatcggca atggcgttaa gaacaagctc
     5041 gtgcgaaacg ccgctgcgaa cttcactctt accaattgcc aacggaagaa ttaagcgcat
     5101 ctctcccgca aggactttct tgtcacgcag catatgcggt aaatacgcct gcgcggacat
     5161 ttcgcgcggc ccattgaccg gtaacccagc ccgcttgagc agggttataa tacgctgcgt
     5221 ttcggcagaa ctaaactgcc cgagacgttc cgacgtccgc gccgccatca ccatacccgc
     5281 agcgaccgct tcaccatgta accaattgcc ataccccatt tcagcttcaa tggcatgacc
     5341 aaaggtgtgt cccagattca gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac
     5401 aacttctgcc ttcagttcac aacaacggcg aatacagtac gccattgccg gaccgtccag
     5461 acgcaacaac gcatccagat tctcttccag ccagttaaaa aacgcaccgt caagaataat
     5521 gccgtatttg atgacttctg ccagccccga cgctaactca cgcgggggaa gcgttttcag
     5581 acagtcgaga tccaccacca ctgaagcagg ctggtagaac gcgccaatca tgtttttacc
     5641 gaggggatgg ttgaccgcag ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt
     5701 cgtcgggact tgaatgaaac gaacaccgcg ctgataactc gccgccgcga agccggtcag
     5761 atcgcccact acgccgccgc caagcgccac cagcgtagta tcgcgaccat gcggtttttg
     5821 taacaacgcc gtaaagacgg tatcgagtac agccaggctt ttatactgct cgccgtcagg
     5881 gaggataacg ctatcgacgt taacacccgc ctgttcaagt acgccgcgga ccttatcgag
     5941 atacagagga gccagggttt cgttggtgac caacatgacc tgctcgcccg atttcagcgg
     6001 taagaatgaa gctggttcat taaacaaacc agatgcgatg gtaattgggt aactacgttc
     6061 cccgagagta acgacaatcc tctccataac gcgacatcca ccttaattac tgtacccgca
     6121 gacgagtgta tataaagcca gaattagttg ctttccagca tgtgaataat ctggtttgca
     6181 accactttag cgctttgatc atcagtacga atggtcacgt cggcaatctc ttcatacagc
     6241 ggattgcgtt cattggccaa cgcttccaga acttcacgtg gcggtgtttc aacgtgcagc
     6301 aacgggcgtt ttttatcacg ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga
     6361 taaacgacaa cgccacgagc ggaaagacgg ttacgcgttt cacgggattt cacagagccg
     6421 ccgccagtag ccagcacaat accctgtttc tcggtcaact cattgatgac cttttcttcg
     6481 cgatcgcgga agccttcttc gccttctaaa tcgaaaaccc agcccacatc agctccggtt
     6541 cgtttctcaa tctcttgatc ggaatcgtaa aattccatat tgagttgttg agctaactgg
     6601 cgcccaatag tgctttttcc ggcacccata ggcccaacca gaaagatatt gcgtttctct
     6661 gccatttttt cggtactact aagactattc gttaatgata aacccgcttc gctcagagag
     6721 cgccgcagga catgaactga aacctcataa gatattgcga gagtcagact gaaaattatc
     6781 tcaatactcc agcgggtttg gcaactgaat aaatcaccca tccctctgca tatctggtcg
     6841 ctgcaagcgc gctgccttgc taccaccgct ctggcgataa atcaccgggt aagattagcg
     6901 taaaaaagac agcaaaatgc cgcctgaatg ataaatcatc atcatggaac gcaggcagcg
     6961 aagagcacca actctcaaat cggtactcct tgtatgctaa atacctgcgc gcgtcaaata
     7021 gatgaaacac gttcagcgta aaaacattac cgcttttacg gctgtttact cactggaaac
     7081 cagtcgtggc gtgataaaca ccactaactc gcgtcgttca tcttcttttc cgtcatgacg
     7141 aaataattgc ccgaaccagg gaatgtcgcc aagcaacggc acgctatcct gacccgattt
     7201 atttttacgg gtaaaaatgc cgcccagcgc caacgtttct ccgcttttga cctcgacctg
     7261 cgtttcgatc tcctgcttat caatcgccag cacttcgcca tcggcctgct gtagcacctg
     7321 ccccggaacg ttctggctga tgtgtaattt cagccggatg cgaccttttt gtaacaccgt
     7381 gggcgtgacc tccatcccca ggactgcctc tttaaattcc accgacgtcg cgccactttc
     7441 cccgctggaa acttgatatg gaatttcgct cccctgttta atgctggcag gctgaagatg
     7501 tgaggccagc agacgcggac tagcgataat atccagctgc tgtttttgtt cgagcgcaga
     7561 aagctcaaga tccagcaaac gtccgttgat gcgcccaatg ttaaaaccga catgcgttgt
     7621 cgccgtcgct acggagaggt cgctgccaag cgtggtgact tgcccaacgc caccagcttg
     7681 ttgcgcatcg gccagcgtcc atttcacgcc taactcacgc aaactttttt cattaatggt
     7741 gacaatatgc gccgacagct caacctgccc gaccggcaga tccatttgcg ctacccactg
     7801 ttcaagggcg cttaacgccg ttttgttatc tcgtagcaaa aggcgattgg tgcgtttatc
     7861 gacggtcata ctccctttgg tactcagtag cttctcccca gctttcgcca gctctccggc
     7921 gtcggcgtat tgcagagtaa tattgcgatt ttccagcggc agatttgcct gcgcccgcgc
     7981 ctgctccgct tcctgacggg cgatattgtc attctgccag gcaatggaat gcactgagag
     8041 aatgttgccc tcctgacgcg ttatcagtcc ggcgcttttc actacagttt gtagtgcctg
     8101 cttccaggga acatctgtta gatgtaacga caccgtaccg ctgacgtctg gcgacaccac
     8161 caggttcaac ttctcctgtt cagccagcgc ctgcaacacc tgagctaccg gaacatcatc
     8221 caccatcagc gtcacttttt gcggctttgc cgcctgtacg ccgggtatca gcatcaacag
     8281 tagtgcggct atccattgct tcatttgtat ctccttgccg ttgccacaac catcgtggcg
     8341 gttcgcagtt tgtcccggta cccagcgtta gtacgtctgg cgtcagctgt aaaattgtcc
     8401 agccgttttc cagcacatcg ttttgctgca cccgtcgcca tttcttttgc ccgtctttta
     8461 ttacaccgat gatgcgctcg cctcgcccta ccatcccctg atagcgccac tggctaagtt
     8521 cgctaatccg gcatagatct tccggcggtt taaaagggtc acgcataccg gttaaaaggc
     8581 acaatgcaat acctgccaac aaccagcgtt taaccctcat gcggcgtctc cagttgtagc
     8641 gtgaacaaaa gatcatcacc ttccacgctt aatgaaaaac ggctcacgct gacgttgcgc
     8701 tctgccagcc gtgtaaatgc cgacggcact gcttcccaca gcgttttcaa cgccaactcg
     8761 cctccctgcg cggatggatg ccagaaaacc agttgtgcgc cgggcaactg aaaatccagt
     8821 ggcgaaaagg gcagcgtttt ttcttcgctg aagggagtgg tgtcaaccag gcgatacagt
     8881 gtggcccact gctgatgatg acttacccgt agtcgaatta atgcgtcgcg ctcttcatgg
     8941 tgtgtcgatg acagaaaaat gagcgtaact aacatcagca accagactgc ccagcaaagc
     9001 tggcggagac ggggggatgt ggcgaaccac cagtcaaaga acatgttcat cgctaacctt
     9061 ccttgttaac tgatactcaa attgccagcg tccctgcgca tcctgctgcg tggccccccg
     9121 ctgattgaga tgaaaagatg catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt
     9181 aatgcttgtt gtaagcccct tgatctccag cgttccctgc tgccagctta tcgttgtcag
     9241 ccaggcgtgc tcgggtaaaa gcgccgccag tgcttccagc gcagattgcc agtcgcgggt
     9301 aaattgtcgc tggcgctgcc tttgcaaacg ctgctcacga atttgttggc gctccagcaa
     9361 acgtggtttc gttatctgta agctgcgggc gagttgttgt tccccctgaa gcaaaacggc
     9421 gtctacgcga gcttcggcgc tgcttgtcag acgcagtatt agcgttatcc cgacggccag
     9481 cagcagaggc gcaacgaaca tcagcaacca gaaacgaaga aaagcggtcc ggcgttgctg
     9541 tcgccagggc aaaaaattaa ttggcgggtt catcagtacg ccttcccaag cgccagcccc
     9601 agcgcgatgg caaagtctcc acctggcggc ggtaacggcg gctgacgaac agaaacggcc
     9661 tcccagggat caaatccgcc ttcgccacat atcgcgacgc tttcgggatc aacggataac
     9721 gctgccgcca gctccttcgc gctagtcatc cccaccgcca gtttgcgccc ccagcgatag
     9781 cgcgtcgccc acagccactg ttcgttatca cgccaggcca gacattgctg atgagaaggt
     9841 aaaaaaggca ggaatcgctg taatgcactg gcatccgggg tgatcgcact aacatgaaca
     9901 cgcaaccttt ctgccagagt aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg
     9961 ttataggcgg gactgagtga gtcttcgctg taatcgaagc gcagggagtc cggatccata
    10021 tccagctcgc gagccatcgt cccggacagc caggccgttt gctcccgctc accgagggac
    10081 atcgacgggc gcggaaatga ccgctgtaat gtgcgactgg cgggaaacgc caacataatg
    10141 tgatgacgct gcggcagttc gcgactccac ggtaacaacg ttttagccag ccgctgcgca
    10201 tcaacaatcc gcccatcttt gataatgtcg tgcgccaacg gcaaccgcca ccagcgttgc
    10261 aaaaagcatt cttttgcgcc ccgcacaata gcaaccgcta ccgcttcttg ctgttgtaaa
    10321 tgcaaaccaa tttgccagat cttaaatgcc attgtgatga tctccttatc acccgtcact
    10381 ctgacgggta tatcaatgcg tctggcttgc ctttatacta ccgcgcgttt gtttataaac
    10441 tgcccaaatg aaactaaatg ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct
    10501 tgcagtctgt tgcattctgc tgggagcagg ctcgatttat ggcctatacc gctacatcga
    10561 gccacaactg ccggatgtag cgacattaaa agatgttcgc ctgcaaattc cgatgcagat
    10621 ttacagcgcc gatggcgagc tgattgctca atacggtgag aaacgtcgta ttccggttac
    10681 gctggatcaa atcccgccgg agatggtgaa agcctttatc gcgacggaag acagccgctt
    10741 ctacgagcat cacggcgttg acccggtggg gatcttccgt gcggcaagtg tggcgctgtt
    10801 ctccggccac gcgtcacaag gggcaagtac cattacccag cagctggcga gaaacttctt
    10861 cctcagtcca gaacgcacgc tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat
    10921 tgaacagttg ctgacgaaag acgaaatcct cgagctttat ctgaacaaga tttacctcgg
    10981 ttaccgcgcc tatggtgtcg gtgctgcggc acaagtctat tacgggaaaa cggtcgacca
    11041 actgacgctg aacgaaatgg ctgtgatagc cgggctgccg aaagcgcctt ccactttcaa
    11101 cccgctctac tcgatggatc gtgccgtcgc gcggcgtaac gtcgtgctat cgcggatgct
    11161 ggatgaagga tatatcaccc agcaacagtt cgatcagaca cgcactgagg cgattaacgc
    11221 taactatcac gcgccggaga ttgctttttc tgcgccgtac ctgagcgaaa tggtgcgcca
    11281 ggagatgtat aaccgttatg gcgaaagtgc ctatgaagac ggttatcgca tttacaccac
    11341 catcacccgc aaagtgcagc aggccgcaca gcaggcggta cgtaataacg tgctggacta
    11401 cgacatgcgc cacggctatc gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggt
    11461 gtgggataac aacaagatta ccgatacgct gaaggcgctg ccgacctatg gtccgctgct
    11521 gcctgccgca gtcaccagcg ccaatcctca ggaagcgacg gcgatgctgg cggacgggtc
    11581 gaccgtcgca ttgagtatgg aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca
    11641 gcagggaccg acgccgcgta aagtgaccga tgttctgcaa acgggtcagc aaatctgggt
    11701 tcgtcaggtt ggcgatgcat ggtggctggc acaagtgccg gaagtgaact cggcgctggt
    11761 gtcgatcaat ccgcaaaacg gtgccgttat ggcgttggtc ggtggctttg atttcaatca
    11821 gagcaagttt aaccgcgcca cccaggcact gcgtcaggta ggttccaaca tcaaaccgtt
    11881 cctctacacc gcggcgatgg ataaaggtct gacgctggca agtatgttga acgatgtgcc
    11941 aatttctcgc tgggatgcag gtgccggttc tgactggcag ccgaagaact caccaccgca
    12001 gtatgccggt ccaattcgct tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt
    12061 acgcgcaatg cgggcgatgg gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt
    12121 cccggcgcaa aacattgtcc acaccgaatc gctggcgctg ggttcagcgt ccttcactcc
    12181 aatgcaggtg gcgcgcggct acgcggtcat ggcgaacggc ggcttcctgg tggacccgtg
    12241 gtttatcagc aaaattgaaa acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt
    12301 agcctgcccg gaatgtgata ttccggtgat ttacggtgat acgcagaaat cgaacgtgct
    12361 ggaaaataac gatgttgaag atgtcgctat ctcccgcgag cagcagaatg tttctgtacc
    12421 aatgccgcag ctggagcagg caaatcaggc attagtggcg aagactggcg cgcaggagta
    12481 cgcaccgcac gtcatcaaca ctccgctggc attcctgatt aagagtgctt tgaacaccaa
    12541 tatctttggt gagccaggct ggcagggtac tggctggcgt gcaggtcgtg atttgcagcg
    12601 tcgcgatatc ggcgggaaaa ccggtaccac taacagttcg aaagatgcgt ggttctcggg
    12661 ttacggtccg ggcgttgtga cctcggtctg gattggcttt gatgatcacc gtcgtaatct
    12721 cggtcataca acggcttccg gagcgattaa agatcagatc tcaggttacg aaggcggtgc
    12781 caagagtgcc cagcctgcat gggacgctta tatgaaagcc gttcttgaag gtgtgccgga
    12841 gcagccgctg acgccgccgc cgggtattgt gacggtgaat atcgatcgca gcaccgggca
    12901 gttagctaat ggtggcaaca gccgcgaaga gtatttcatc gaaggtacgc agccgacaca
    12961 acaggcggtg cacgaggtgg gaacgaccat tatcgataat ggcgaagcac aggaattgtt
    13021 ctgattaaaa aggcgcttcg gcgccttttt aagtttgctg acaaagtgca ctttgtttat
    13081 accggatacg gcgtgaacac tttacccgtc tagcaacatc tagaaaaatc aatatattgc
    13141 agaaacttcg taagcctgat aagcatcagg caaacttcac gcatttacac tcgcccctgc
    13201 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    13261 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    13321 ggctcatcgc cttccagcga ttccgggtag agatcttgcg ctaccacgat attcattttg
    13381 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    13441 aatccaacct cttcttttag ctcacggtta gcggcttcga agacgctttc acccggatca
    13501 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcata ttcgcggatc
    13561 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    13621 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    13681 aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    13741 ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    13801 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat
    13861 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga
    13921 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac
    13981 gcttgccgat agccaaccgc agaatcatgt attgtgtccg gtgcgactga ccacgcctga
    14041 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    14101 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    14161 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    14221 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagcc
    14281 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    14341 cacgctatca cacgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    14401 gattcggtag aagtccacct gccccccttc tgggaacagt acatcaacga tgagaatacc
    14461 gttgaactga ttcataccga ttcgctgccg ttggttattt cactcaacgg tcatacgctg
    14521 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    14581 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    14641 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    14701 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggt
    14761 ggcgcgttac tgctgctggg cgcaggtctg tgggggctat tcgccccccc ggcaaaatcc
    14821 tccctgcggg aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    14881 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    14941 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatatcga tatctatctc
    15001 gaccgccatg tagtgcgtca gggacgatat ctttcgctgc atgatgaagt aaaaaacttt
    15061 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt
    15121 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    15181 ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    15241 ggcgatacat tgcgtattag tggtacggga atgtgtaata ttcgcacttc cggaacctgg
    15301 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac
    15361 gcccgctcat taccattacc agaatctgag ctggtcaaca aagcgacggc attgactgaa
    15421 gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta
    15481 cgttcagcga ttcaaaaatc cggcatggta ttgattgatg attttggcga cattgtactg
    15541 aagacagcgg atctgtgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    15601 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    15661 gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca
    15721 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    15781 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    15841 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    15901 ctggcacaaa tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc
    15961 actgacgcca atggtacgca gcatataggc cttcatccga tcccggatcg ttccggcctg
    16021 tggcgctatc tcagcaccac actgctgcta ctgacgatgc tgggtagcgc catttataat
    16081 ggcgtacagg cctggcggcg ttaccagcgt catcgcactc gcatgatgaa gattcaggcc
    16141 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    16201 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttt
    16261 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    16321 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    16381 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tttatgcgcc agcaatatca
    16441 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    16501 ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat
    16561 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca
    16621 tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt
    16681 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    16741 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    16801 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgactcagg
    16861 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    16921 ctcgctaatg tgaaggagag gccatgaaag aaaaacctgc tgttgaggtt cgactggata
    16981 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    17041 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    17101 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    17161 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    17221 agaaacgcga aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg
    17281 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    17341 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    17401 tgtttgaaaa ctttgccgtg cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    17461 tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    17521 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcagg
    17581 gcgacggtcc gatgaatctg gcagttatta acggtaacaa taaccagcaa atgcgcggtg
    17641 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    17701 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtagttggtc
    17761 tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc
    17821 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    17881 tgttgcaggt aatgcctgcg caaaatgccc agcaggacga cttcgaccac ctggcgacgc
    17941 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    18001 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    18061 cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagtcgata
    18121 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    18181 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag
    18241 ttcattaatg agtatgtccg gcagagagag ctctgccgga ttttacgact aatacacctg
    18301 atgcgctttg tcactcattc agattcacat ccagcctttc accatccact acaagatcgc
    18361 gccacgcttt aggcttcgtt cccagttttc catccttcgc agctgtgagt aatttttctt
    18421 tcgttatatc acgcggaagt aaactcatcg taagctcttg ccattctttc ttgtcaggat
    18481 caaagccata gacaatgtat ctttcatcat caaaggcaaa gaggatccgc tctgccctgc
    18541 catcactatt cagatcttgc tcgaccagta cgcaggcgtc tttttcaata caggtcatca
    18601 cgttatagcg atcctggatt aaggccgacc agaatgtcgc atccggttta ccagaaccag
    18661 gggcaattaa cacattttcg gctaatactt tttccgatat ctgttgctga agatgttgct
    18721 ctccatctaa cgccatcagt agatcccgcg cacgtttcgg gtctttcata tactcggcat
    18781 cgcttttcag cgactcaagc gccgcacgtc catagcgacc gctgtgttcg agcatgtaga
    18841 tagttacctg gtctggcgtg tttttgccgc tctgataacg cgccatatga ctgttcacac
    18901 taatgcgcat actgtccagc accggcgaat taagaagcac cagtattacc aacaccaata
    18961 acgaaaccgc aaggttcact ttgccctgaa gaacaagggg attttgccct ttacgccaga
    19021 cgatactgac aaaatacccc agcgaccata ccagtaacac cagcaccgcc agcgcgccct
    19081 gcaagcggtc gacagtccag ccgtactgag cgacccgcag ccataacgcc caggcggcga
    19141 tgaacacata cagcggagca accagcagag cggttttaat caggcaacgc aacggccccg
    19201 tccagggaag tgacgctttt tgcggatcgc ggacaatagc cattaagatc aattgcaaaa
    19261 aggccagcgt taacagcaac ccggcggcgg agatgtggcg agaaatcgcg ctcaggcccg
    19321 taaacggcag ggtgatgata aacatcaggg ttagcaatga tactaacggc agcaaccccg
    19381 tggcgattag cgtgaacaac ttttgaatag agtcgattaa acgtgactgt gttcgcgcga
    19441 ggatcaccgc cagcgcggta accagaccta atgtaagata cataaaccag tcggttgcaa
    19501 aaaagagcgt attgaaaaac gtgatgccaa caagtttaaa caactcaccc cacagtaaaa
    19561 gcaccagcca cgtcaggcca ttggcgagaa aaataacctg taatataagt acgttatgcc
    19621 atactgactg gtaaaaatag caatagcggc tactgtcatt gcggatacga aggctttgtt
    19681 gtatccacgg tagcaacaac attgccatca gcagcagata gcagccaaaa tcccaaagcg
    19741 ccttttcagc tctccagggg ttcatgccat cagtttgcca cttcaaccag ccgctcatcc
    19801 ccagcgtggc aataaacacc agcgccagcc atccccaaag gcgcttctgt ttaaaagaga
    19861 tcacggagaa gagtaaaacg gatgaaaacg caacggtcgc aggcacgcca taaacaatcc
    19921 aactgtgatt ttttccggca agccaggcga tcagcagata gcaaaccagc ccctgaagta
    19981 atccggtagc aatcatcccc cagcgtgttg cgggtgaaag ttcaacgtta tccatgttgg
    20041 ttatccagaa tcaaaaggtg ggttaattat cgcatccggg cagtagtatt ttgctttttt
    20101 cagaaaataa tcaaaaaaag ttagcgtggt gaatcgatac tttaccggtt gaatttgcat
    20161 caatttcatt caggaatgcg attccactca caatattccc gccatataaa ccaagattta
    20221 accttttgag aacattttcc acacctaaaa tgctatttct gcgataatag caaccgtttc
    20281 gtgacaggaa tcacggagtt ttttgtcaaa tatgaatttc tccagatacg taaatctatg
    20341 agccttgtcg cggttaacac ccccaaaaag actttactat tcaggcaata catattggct
    20401 aaggagcagt gaaatgcgcg ttaacaatgg tttgaccccg caagaactcg aggcttatgg
    20461 tatcagtgac gtacatgata tcgtttacaa cccaagctac gacctgctgt atcaggaaga
    20521 gctcgatccg agcctgacag gttatgagcg cggggtgtta actaatctgg gtgccgttgc
    20581 cgtcgatacc gggatcttca ccggtcgttc accaaaagat aagtatatcg tccgtgacga
    20641 taccactcgc gatactttct ggtgggcaga caaaggcaaa ggtaagaacg ataacaaacc
    20701 tctctctccg gaaacctggc agcatctgaa aggcctggtg accaggcagc tttccggcaa
    20761 acgtctgttc gttgtcgacg ccttctgtgg tgcgaatccg gatactcgtc tttccgtccg
    20821 tttcatcacc gaagtggcct ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag
    20881 cgatgaagag ctggcaggtt tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac
    20941 taaccctcag tggaaagaac agggtctgaa ctctgaaaac ttcgtggcgt ttaacctgac
    21001 cgagcgcatg cagctgattg gcggcacctg gtacggcggc gaaatgaaga aagggatgtt
    21061 ctcgatgatg aactacctgc tgccgctgaa aggtatcgct tctatgcact gctccgccaa
    21121 cgttggcgag aaaggcgatg ttgcggtgtt cttcggcctt tccggcactg gtaaaaccac
    21181 cctttccacc gacccgaaac gtcgcctgat tggcgatgac gaacacggct gggacgatga
    21241 cggtgtgttt aacttcgaag gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc
    21301 ggaacctgaa atctacaacg ctatccgtcg tgatgcgttg ctggaaaacg tcaccgtgcg
    21361 tgaagatggc actatcgact ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta
    21421 tccgatctat cacatcgata acatcgttaa gccggtttca aaagcgggtc acgcaacgaa
    21481 agttatcttc ctgaccgcag acgcctttgg tgtgctgccg ccggtttctc gtctgactgc
    21541 cgatcaaacc cagtaccact tcctctctgg cttcaccgcc aaactggccg gtactgagcg
    21601 tggcatcacc gaaccgacgc caaccttctc cgcttgcttc ggcgcggcat tcctgtcgct
    21661 gcacccgact cagtacgcag aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc
    21721 ttatctggtt aacactggct ggaacggcac tggcaaacgt atctcgatta aagatacccg
    21781 cgccattatc gacgctatcc tcaacggttc gctggataat gcagaaacct tcactctgcc
    21841 gatgtttaac ctggcgatcc caaccgaact gccgggcgta gacacgaaga ttctcgatcc
    21901 gcgtaacacc tacgcttccc cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact
    21961 gtttatcgac aactttgata aatacaccga cacccctgcg ggtgccgcgc tggtagcggc
    22021 tggtccgaaa ctgtaatgac ttgaagctgg agaatatcta tccagtatct tatagaaagc
    22081 aaaacgggag gcaccttcgc ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc
    22141 gcgttaccgg cacaggcagc caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa
    22201 gctccagcat cccgttatgg ttatccacga tacgctgcac aatcgccagc cctaatcccg
    22261 tgccgctaat ggtgcgtgca ctgtcgccac gaacaaacgg ctggaaaagg tgcttacgtt
    22321 gttccggcgc aattcccgga ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg
    22381 tcccgctgct gactttgatc cagccattgc cgtaacgggc ggcgttgacc accatattcg
    22441 ccaccgcgcg tttgatcgac agcgggtgca ttttcacttc aatgctgccg gggtaaagcg
    22501 cggtttcaat ttcccgctca tagccacttt cggcagcaat aacctcaccg agtactgcgt
    22561 tgagatccgc catttccatc ggcatctcct gcccggtgcg caggtagtcg ataaactgct
    22621 caatgatggc gttgcactct tcgatatctt tattgatcga ttctgccagg tagccatcct
    22681 gctcgctcat catctcggtc gccaggcgaa tacgcgtcag cggtgtacgc aagtcgtgac
    22741 ttacccccgc catcagcagc gtgcggtcat ccgccagttg cttaacacca gccgccatat
    22801 ggttaaaggc gcgggtaacg gaacgcacct cggacgcgcc atactcacgc agcggcggcg
    22861 gaataatccc tttaccgacc tgcaaagcag catgttcgag atcgaccaac ggtcggttct
    22921 ggatacgaat aaacagccac gccccgccta tcgccaatag cataatcgcc aacgtatagc
    22981 ggaacagcgg agagaaatcg ccctgatgaa tttcggtcag cggcacgcgt acccagatat
    23041 tgggcgacag ccaagttttc agccagacga caggcgaact tttgttgacc tcaacgcgca
    23101 cttccgtcgg gccgcccagt tgctgcgcca tctgatggct taagaattca tagtgttgcg
    23161 cccaacgcag acctgcctct tcggcagcct cgttggagta gagagagatc cccagctcac
    23221 ggtagatctc ccgacggaaa gcgggaggca caaccaactg cgtgccgtcc tccagttgca
    23281 gtttgtcggt catcaacata cgcacttcgt acgcgaggac tttattaaac tgctggaggc
    23341 tcggcaaaat cgcgaagttc agcaccacca gataagtcgt caccaggctg gcgaacagca
    23401 aggtgacgat gagcaataac gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc
    23461 tcatgcttta gagccgtccg gtacaaagac gtagcccaag ccccaaacgg tctgaatgta
    23521 acgcggatgc gctggatctt cttccaccat gcggcgcaga cgcgaaatct gcacgtcgat
    23581 ggagcgttcc attgcggaat attcacgacc acgggcaagg ttcatcagct tatcgcggga
    23641 gagcggctca cgcggatggc tgaccagtgc cttcagtacc gcaaactcac cgctggtgag
    23701 cggcatcggc tcgtcttcgc ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc
    23761 gaaagcaatt accgcctctt cctgtgacgg tgcgcctggt agctcgttcg cctgacgacg
    23821 cagcaccgca cggatacggg ccagcagttc acgcgggtta aacggttttg gaatgtaatc
    23881 gtcagcgcca atctccaggc caacgatacg gtccacttct tcccctttcg ccgtcaccat
    23941 aatgatcggc atcgggttgc tctgactacg aagacgtcgg caaatcgaca agccatcttc
    24001 accaggtaac atcaaatcca gtaccataag atggaaagat tcacgagtca gcaggcgatc
    24061 catctgttct gcattagcga cgcttcgaac ctggaagcct tgttcggtga gataacgctc
    24121 cagcagcgcg cgcaggcgca tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat
    24181 tgtttgtact cccaaaggtt cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg
    24241 ttcgtcacca gctaaatctg gtatgaattt cagcctaaat tgttacaaag catattaaac
    24301 agcagcttaa gtatacaatt tattcggcga aacattattg attctgttga tatgatcacg
    24361 ttatacccaa tgtgcgcatt atcaaacaga caaagggaat caacgagatg aaaacgcccc
    24421 tggttacccg ggaagggtat gaaaaactca aacaagagct taattatctc tggcgtgaag
    24481 aacgcccgga ggtcacaaaa aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa
    24541 atgctgacta tcagtataat aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc
    24601 tcactaaatg catggaaaat ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag
    24661 tcttttttgg cgcgtgggtg gagattgaaa acgacgatgg cgtgactcac cgtttccgta
    24721 ttgtcggcta cgatgaaatt tttggccgta aagattacat ctctatcgat tccccgatgg
    24781 cccgcgcatt gctgaaaaaa gaagtcggcg atctggcggt ggtgaatacc cctgccgggg
    24841 aagcgagctg gtatgttaat gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct
    24901 cctgccgatg gctggcattt ttgccagcca gtccgtataa ctatcccctg atttttgatc
    24961 cgaaaagatg aactcaaacc atgatgaatg attcgttctg ccgcattatt gcgggtgaaa
    25021 ttcaggcgcg cccggaacag gttgacgctg ccgttcgcct gcttgacgaa gggaataccg
    25081 tgccttttat tgcacgttat cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc
    25141 gtaatctgga aacgcgtctg agctatctgc gcgagctgga agagagacgt caggcgatcc
    25201 tcaagtccat ttccgagcaa ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca
    25261 ccttaagcaa aaccgaactc gaagacctct acctgcccta caaacctaaa cgccgcaccc
    25321 gcgggcaaat cgccattgaa gcagggcttg agccgttggc tgacctgctg tggagcgatc
    25381 cgtcacacac gccagaagtc gccgctgcgc aatatattga tgcagataaa ggcgtggcag
    25441 ataccaaagc cgcgctggac ggcgcgcgct atatcctgat ggaacggttt gccgaagatg
    25501 ccgcgctgct ggcgaaagtg cgtgattatc tgtggaagaa cgcgcatttg gtttcgacgg
    25561 tggtgagcgg taaagaagag gaaggggcga aattccgcga ctatttcgat catcacgaac
    25621 cgttgtccac ggtgccttct caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg
    25681 tactccagct ttcgctgaat gccgatccgc agttcgatga gccgccaaaa gagagctatt
    25741 gcgagcaaat catcatggat catctcggcc tgcgcctgaa caatgctccg gcggatagct
    25801 ggcgcaaagg cgtggtgagc tggacctggc gcatcaaggt gctgatgcat ctggaaaccg
    25861 aactgatggg caccgtgcgc gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta
    25921 acctgcacga tctgctgatg gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc
    25981 cgggtctgcg tactggggta aaagtggcgg tggtcgatgc cactggcaaa ctggtagcga
    26041 ccgataccat ttacccgcac accggacaag ccgcaaaagc agcgatgact gttgctgcgc
    26101 tgtgtgaaaa gcataacgtt gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa
    26161 ccgaacgttt ctatctcgac gtgcagaagc agttcccgaa agtgaccgca cagaaagtga
    26221 tcgtcagcga agctggcgcg tcggtttact cggcttccga gctggcagcg caggagttcc
    26281 cggatctcga cgtttcgctg cgtggcgcgg tttctatcgc ccgccgtttg caggatccgc
    26341 tggcggagct ggtgaaaatc gatccgaaat ctatcggcgt aggtcagtat cagcatgacg
    26401 tcagccagac gcaactggcc cgcaaactgg atgcagtagt agaagactgc gtaaacgccg
    26461 ttggcgtcga tctcaacacc gcttctgttc cgctattaac tcgcgtggcg ggcctgacgc
    26521 gcatgatggc gcaaaacatc gtggcctggc gcgatgagaa cggccagttc cagaaccgcc
    26581 agcaactgct gaaagtcagc cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc
    26641 tgcgcattaa ccacggtgac aacccgctgg acgcctctac cgttcacccg gaagcctatc
    26701 cggtggtgga acgcattctg gcagcaacac agcaggcact gaaagatctg atgggtaaca
    26761 gcagcgaact gcgtaacctg aaagcgtctg actttactga tgaaaaattc ggtgtgccga
    26821 cagtaactga catcatcaaa gagctggaaa aaccgggtcg cgatccgcgt ccggaattta
    26881 aaaccgctca gtttgccgat ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc
    26941 tcgaaggcgc tgtcaccaac gtcaccaact ttggcgcgtt tgtcgatatc ggcgtgcatc
    27001 aggacggcct ggttcacatc tcttcattgt cgaacaagtt tgtggaagat ccgcataccg
    27061 tggtgaaagc gggcgacatt gtgaaggtga aagtgctgga agtggatctt cagcgtaaac
    27121 gtatcgccct gactatgcgt ctggacgagc agcctggcga aaccaactct cgtcgcggcg
    27181 gcggtaacga tcgcccgcaa aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc
    27241 agcctgccgg taacagcgcg atgatggacg cgctggcggc agcaatgggc aaaaaacgtt
    27301 aaacgcccgg actggcccct aaacctgcgc gataaggcgt tcacgccgca tctggcaacg
    27361 aacgccttgc ctgacataaa agtgccggag aatatctccg gcatatttat tccacagcca
    27421 aactcacaat atatttcgtt aatatttatc acttcattaa caactgaaac cttaattaaa
    27481 cattagccag tcctggtaat tcgctattcg aattatattt tcgctgcgat ataaccttga
    27541 gccacatcaa cattgagtca gattattatt caaaccaaca ttcgcacaca ttttaagtat
    27601 tgctgataga aaccattctc attatcattg tactgttgat tatttaatct ctccttcgtt
    27661 ggcaaatcat ctggtctcat gtcgctgtca aacgccccat gaggtagtta tccagttaat
    27721 gagaaacaag taggcaccta tgcaatacac tccagatact gcgtggaaaa tcactggctt
    27781 ttcccgtgaa atcagcccgg catatcgcca aaaactgctt tctcttggca tgttacctgg
    27841 ctcctctttt aatgtggtgc gcgtcgctcc actcggcgac cccattcata tcgaaacccg
    27901 tcgtgtgagc ctggtattac gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc
    27961 ctgttaatac ggtgataaca acaatgaaaa aattaaccat aggcttaatt ggtaatccaa
    28021 attctggcaa gacaacctta tttaaccagc tcactggcgc acgtcagcgt gtaggtaact
    28081 gggctggcgt taccgtcgaa cgtaaagaag ggcaattctc caccaccgat catcaggtca
    28141 cgctggtgga cctgcccggc acctattctc tgaccaccat ctcatcgcag acctcgctcg
    28201 atgagcaaat cgcctgtcac tacattttga gtggcgacgc cgacctgctg attaacgtgg
    28261 tggatgcgtc taaccttgag cgtaacctgt acctgacgct acaactgctg gaactcggca
    28321 ttccctgcat cgtggcactg aacatgctcg acattgccga gaagcaaaat attcgtattg
    28381 aaattgatgc tctgtcggcg cgtcttggct gcccagtgat cccgctggtt tcaacccgtg
    28441 gtcgcggtat tgaagcgctc aagctggcga ttgatcgcta taaagctaac gagaatgtgg
    28501 aattggtgca ttacgcacag ccgctgctca acgaagcaga ttcactggca aaagtgatgc
    28561 cttccgacat cccgctgaaa caacgtcgct ggctgggcct gcaaatgctg gaaggcgata
    28621 tctacagccg cgcctacgcc ggtgaagcgt cgcagcatct ggatgccgcc ctcgcccgtc
    28681 tgcgtaatga gatggacgat ccggcgctgc acattgccga tgcgcgttac cagtgcattg
    28741 ctgccatctg tgatgtggta agcaacaccc tgacggcaga acccagccgt ttcaccactg
    28801 cggtagataa aatcgtgctc aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt
    28861 acctgatgtt cctgctggct atcaacatcg gcggggcgtt acagccgctg tttgacgtcg
    28921 gctccgtggc gctatttgtg catggtattc aatggattgg ctacacgctc cacttcccgg
    28981 actggctgac tatcttcctc gcccagggcc tgggtggcgg tattaacacc gtgctgccac
    29041 tggtgccgca gatcggcatg atgtacctgt tcctctcctt ccttgaggac tccggctata
    29101 tggcgcgtgc ggcgtttgtg atggaccgcc tgatgcaggc gctgggcttg ccggggaaat
    29161 cctttgtgcc gctgatcgtc ggtttcggtt gtaacgtacc gtcggtaatg ggtgcacgta
    29221 cacttgatgc accgcgtgaa cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg
    29281 gcgcgcgtct ggctatcttc gcagtattcg cggctgcctt cttcgggcag aacggtgcgc
    29341 tggcggtctt ctcgctgtat atgctcggta tcgtgatggc ggtgctgact ggcctgatgc
    29401 tcaagtacac catcatgcgc ggtgaagcga cgccgtttgt catggagctg ccggtctacc
    29461 atgtaccaca cgttaaaagc ctgattatcc agacctggca gcgcctgaaa ggcttcgttc
    29521 tgcgtgctgg taaagtgatt atcatcgtca gcattttcct gagcgctttc aacagcttct
    29581 cgttgagcgg gaaaatcgtc gataacatca acgactcggc gctggcttcc gtcagccggg
    29641 tgatcacccc ggtcttcaag ccgattggcg tgcatgaaga taactggcag gcaacagttg
    29701 gcctgtttac gggtgcaatg gcgaaagaag tggtggtggg tacgctcaac accctctaca
    29761 ccgcagaaaa tattcaggac gaagagttca atccggcgga atttaacctc ggtgaagagt
    29821 tgttcagcgc ggtagatgaa acctggcaga gcctgaaaga caccttcagc cttagcgtac
    29881 tgatgaaccc cattgaagct agcaaaggcg acggcgaaat gggtaccggg gcgatgggtg
    29941 tgatggatca gaaattcggt agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc
    30001 tgtatgtacc gtgcatctcg gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga
    30061 tgggcttctc catcctgtgg gggctgaata tcgcttactc gctggcaaca ttgttctatc
    30121 aggtcgccag ctacagccag catccaactt acagcctggt gtgcattctg gcggttatcc
    30181 tgtttaacat cgtggttatc ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac
    30241 tgctggcaac ccgcaagtcg gtaagtagtt gctgcgcagc cagcaccacc ggtgattgcc
    30301 attaatggct tcacttattc aggtgcgtga tttgttggcg ttacggggcc gtatggaagc
    30361 ggcccagata agcaagacat tgaacactcc acagccaatg attaacgcca tgctgcaaca
    30421 actggaaagt atgggcaaag ccgtgcggat tcaggaagaa cctgacggct gcctctccgg
    30481 cagttgtaaa agctgcccgg aaggaaaagc ctgtctgcgc gagtggtggg cgctgcgtta
    30541 accatactcc atcgcctgtt ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc
    30601 gtgcggcttt ccataaaaat gcaattcttg cagcgcgacg taagttcctc tgaaagcgtc
    30661 tcgcagggat gaaaactggc taatgcacag gtgtggagtg gcgcgtagag tcgcggcatt
    30721 caaacaacag gtgaaggaac gccatgagca aaaagcagag ttccacccca cacgatgcgc
    30781 tgttcaaact atttttacgc caaccggaga cggcacgcga ttttcttgcg tttcatttac
    30841 cggcacccat tcacgcgctc tgtgatatga aaaccctcaa gctggagtcg agcagcttta
    30901 ttgatgacga tctgcgtgaa agctattccg acgtgctgtg gtcggtgaaa acggaacaag
    30961 gaccaggata catctattgt ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat
    31021 tccgcatgat gcgttacgcc attgccgcaa tgcaaaatca ccttgatgcc ggatacaaaa
    31081 cgttgccgat ggtggtgcca ttgttgtttt atcacggtat tgaaagcccc tatccctatt
    31141 cgctgtgttg gctggattgt ttcgccgatc ccaacctggc aaggcagctc tatgcctctg
    31201 catttccgct gattgatatc accgtcatgc ctgatgatga aatcatgcag caccgacgca
    31261 tggcgctgct ggagttaatt caaaaacata ttcgtcaacg cgacctgatg gggctggtag
    31321 aacaaatggc ctgcttatta agtagtggat acgctaatga cagacaaatc aaagggctgt
    31381 ttaattacat actgcaaacc ggcgatgcgg tacgttttaa cgattttatc gacggcgttg
    31441 ccgaacgttc accgaaacac aaggagagtt taatgactat tgcggaaaga ttgcggcagg
    31501 agggggaaca atccaaagcc ttgcatatag ccaaaataat gcttgaatcc ggagtccctc
    31561 ttgcagacat catgcgcttt accgggctgt cagaagaaga gttggctgcg gcgagtcagt
    31621 aaagttctgt ctcgccattc aaaagtgcct acaccctctg cttcaacgcc accagcaggt
    31681 gacaaaactc gaccggatgc gaaataaacg gcgcatgggc cgctttggcg aagatatacg
    31741 actcgctgtg cggccagagt ttatccagca tcggcaccac tttgcgcggc accagaccgt
    31801 cgagataacc atacagtcgc aaaaacggca tgggtacgtt ttgcagcggc tggcggagat
    31861 cgaccgtttt caggatttcc agcccaccat tgagcacgtc aacctccggc atcggtaacg
    31921 ccagaacggt tttcttcaac gcccgcgcat cctgacgcgc cgtttcagta cccatggttt
    31981 gtaacgcaag gaaccgttcc accgtacgct gaaaatcatc actgagttgc tgctgaaatc
    32041 ccgccagcac gtccggtttt atccccggcc actcttcacg agcgctgaaa cacggcgacg
    32101 acgccacggt gaccagcgcc tgaacacgct cgggatgggt taacgcaatc tggcttgcca
    32161 ccagcccgcc cagactccag cctaaccaaa tggctttatc aggtgcctgt cgcagcacgg
    32221 cttcggccat atcagcaagt gacatcgcac caaatccccg gctacgcccg aagccgggca
    32281 ggtcaacaag atgcagcgta aaatgcgagc taagttcctc gtcaatgcaa cgccacactt
    32341 cggcattcag tccccatccg tgcagcagca caagatgaac attcccctga cctttggtct
    32401 gccaccagat gttattcatc cgctattgtt ctcttttgac ttacaaggat gaacatatgc
    32461 taacagtacc gggattatgc tggctatgcc gaatgccgct ggctttaggt cattggggga
    32521 tttgttcggt ctgctcacgc gccgcccgta cagataaaac gttatgccca caatgtggat
    32581 tacccgccac acactcccat cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc
    32641 aaagactggt cacggttgcc gactatgcgc cgccgttaag tctgctcatc caccagctta
    32701 agttttcccg ccgcagcgaa atcgccagcg ccctgtcacg tctgttacta ttggaagtct
    32761 tacacgctcg tcgcaccacc gggttgcaat tgccggatcg tatcatcagc gttccgttat
    32821 ggcagcggcg tcactggcgt cggggattta atcagagcga tttgctgtgt cagccgttat
    32881 cacgctggtt gcactgccaa tgggatagcg aagccatcac acgtacacgg gccactgcga
    32941 cccagcattt tcttagcgcc cggctacgca ggcgcaacct gaaaaatgcc tttcgccttg
    33001 aattgcctgt gcaaggtcgc catatggtga ttgtggatga tgtcgttacc accggaagta
    33061 ccgtcgcaga gattgcgcag ttgcttttac gcaatggtgc ggcgactgtc caggtctggt
    33121 gcctttgtcg aaccttgtag agcctcgatg atgggcgtat tataaccaac taaaatagtc
    33181 aactattagg ccattactat gatccgtatt tccgatgctg cacaagcgca ctttgccaaa
    33241 ctgctggcaa atcaggaaga agggacacaa atccgcgtat ttgtgattaa ccctggcacg
    33301 cctaacgctg aatgtggtgt ttcttattgt ccgccggatg ccgtggaagc caccgacacc
    33361 gccctgaaat ttgacctgct gaccgcgtat gttgatgagt taagcgcacc atacctggaa
    33421 gatgcagaga tcgattttgt taccgaccag ttgggttctc agctaacact gaaagcgcct
    33481 aacgccaaaa tgcgtaaagt ggcagacgat gcaccactga tggagcgcgt ggagtatatg
    33541 ctgcagtcgc agatcaaccc acagcttgct ggtcacggtg gccgcgtttc actgatggaa
    33601 atcaccgaag acggttacgc cattctgcaa tttggcggcg gctgtaacgg ttgttccatg
    33661 gtcgatgtga cgctgaaaga agggatcgag aagcagctac taaacgaatt cccggagctg
    33721 aaaggtgtgc gcgatctcac cgaacaccag cgcggcgaac actcctacta ctaagttatc
    33781 ccctcttttg tagattgccc gatgcgacgc taaagcgtca tttcgggcct acgttgaaca
    33841 tgtgccagac gttggcgcag cattgctttg gcaaatccct cccttcgttt ccccgcgata
    33901 atatgaccaa cctctcataa tttaaattta ccccgctctg gtgattctca aacgccagat
    33961 gttacccgta tcattcacat gggtaccaaa catactcctg acatctgact acaataatta
    34021 gttttagtgg gtatcagtcg tggtgccgca atatctctgt tcccgattgg gataattaga
    34081 gtttgtcgtc agaaaattga cgttacccat aacaaatgaa aggccaggta aatcatgcca
    34141 ttagtcattg ttgctatcgg tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg
    34201 aacggcttca tcgctctcgt cctcgtggcg cttgctgttg gattaatgca aggaatgccg
    34261 ctggataaag ttattggctc catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc
    34321 ctgatcatgg gttttggcgc aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa
    34381 cgtatcgcca ccacgctgat tgccaaattt ggtaaaaaac acatccagtg ggcggtggta
    34441 ctgaccggtt ttaccgttgg ttttgccctg ttctatgaag tgggctttgt gctgatgctg
    34501 ccgctggtgt ttaccatcgc ggcttctgcg aatattccac tgctgtatgt tggtgtacca
    34561 atggcggctg cactgtctgt gacccacggc ttcctgccac cgcatccggg tccgactgcg
    34621 attgccacca ttttcaatgc cgatatgggt aaaaccctgc tgtacggtac tattctggca
    34681 atcccgaccg tgattctcgc cggtccggtt tacgctcgcg tgttgaaagg tatcgataag
    34741 ccaattccgg aaggtctcta cagcgcgaaa accttcagcg aagaagagat gccgagcttt
    34801 ggcgtcagcg tctggacctc tctggtgccg gtagtactga tggcgatgcg tgcgattgcc
    34861 gaaatgatcc tgccgaaagg tcacgctttc ctgccggtag cggagttcct cggtgacccg
    34921 gtaatggcta cgctgattgc cgtgctgatt gcgatgttca cctttggtct gaaccgtggt
    34981 cgttcaatgg atcagattaa cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg
    35041 ctgttgatca tcggtggtgg cggtgcgttc aagcaggtgc tggtagacag cggcgtggac
    35101 aaatacattg cttccatgat gcacgaaacc aacatttctc cgctgctgat ggcctggtcg
    35161 attgctgccg tactgcgtat cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt
    35221 ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc cggagctgat ggttattgcg
    35281 gttggttccg gtagtgtgat tttctctcat gtgaacgatc cgggcttctg gctgttcaaa
    35341 gagtacttta acctgactat cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt
    35401 atctcggtgt gcggtctggt aggctgtctg ctgctgaata tggtgatttg ataacactga
    35461 ctgccggatg catcgcatcc ggcatcaacc gcactctact tcttcttcgc tgcagctctg
    35521 cgccgtctgt ccaaatcctt cagcaacttg ttcacgccat catcggcaaa catcgtctca
    35581 agcgttgcgg aaagcttgcg tcgccagttt ttatactggt aactggtgcc aggaatattc
    35641 accggttcgg ccatatccag ccagtcttcc ggctgtagtc ctaacagagc actgttactg
    35701 tcggcaatgt agcgctgcaa accacggttc agcgtcggcg tcatcgacat caacgatgcc
    35761 ttatgcccgg cacgttttgg cagacaacca tatttatgca gtgcatccag cagcccttgc
    35821 ttcgccagtt cgcgatcctg atacagaccg cgcagtacca cttcatccgg atacagcccc
    35881 agggttttgc ccagagttag atccccgctc tcccagtaac cacgcagcgt tggcaggtcg
    35941 tgtgtcgccg caaccgccat cgactgctcc gggtacgctt tcggtgcacg gaacgtcttc
    36001 tcgtggtcgt tttcgaaata gagcactttg taggagtaca caccgctgct gcgcagctta
    36061 ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt
    36121 ttactttcga gtgccagaat cgagagcaga tcatccaccg gatagtgaac atacgcgccc
    36181 tgatctgccg tctcgccata cggtatccac cacaaacgca gcatcgacat aacatggtca
    36241 attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca gctcgataaa cggttcatag
    36301 gcacgcgcgg tgatgatatg cgggtccatt ggcggcaatc cccagttctg ccccaacggg
    36361 ccgaggatat ccggcggcgc gccaaccgac gctttcaggc aatacagttc acggtcgcac
    36421 caggtttccg ccccaccttc cgctacgcca accgccagat cacgatacaa gccaatcggc
    36481 atttcatagc cctggcttat ctcccagcag gcggcaaact ggctgtaagc cagccactgc
    36541 agccagagat aaaaatcaac gtcatcgcga tgttcttcgc agaactgacg cacttctggt
    36601 gaatccacgt tctgatacat ctctggccat gcaggccagc cccagcgcat ttcgtcctct
    36661 ttcacttgct gggcatgtag cgcatcaaag gctgcctgcc agaacaggct gtcgccttga
    36721 tctgcaacaa actggcgaaa cgcggccatc tgctcatcat cacgttgcgc gaaacctttc
    36781 cacgccattc gtaatgctgt catttttagg gcggtaaccg tggagtaatc gacccagtcg
    36841 gcatcccgcg cctgttgcag cgtctgttgc gtggtcggca actgccacca ggcctgagcc
    36901 tcttcgctaa gatggaaatc ttcgacggcg ttaacgtcga tataaatcac attcagccag
    36961 cggcgagaag acgggctgta tgggctggcg ctctccggat ttgccggata gagcgcatga
    37021 atcgggttca ggccgatgaa cgacccgcca cgttttgcca catccaccag catcgctttg
    37081 agatcgccaa aatccccaat accccagttt ttttccgatc gcagcgtata aagctggacg
    37141 caggcacccc acagcttttg tttattcagc aacgcctgcg gttcgtaaca gcgtttcggg
    37201 gcgacaatca ccctgcaatg cgcgcgctgg tcttcctggg taagcgtcag cgtgtgataa
    37261 ccttccggca gcttcgtcgg tagattgaac gctttgcccc cagttacatg gcctttgtac
    37321 tgcgttcctt cttcggtggt cagcagccag ctatattcgc cgctgccctc caccaccatc
    37381 ggcatttttt tgccgctggt ataaaccatg acattcggga ctggcgttac cgccactttc
    37441 gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac
    37501 tgcggtttac cgtgggcatt gatgtaattg gggctaatcc ccgccgccag cgcggcatta
    37561 tccagacgtt tgctttccat cgagcttcct tagcgttttg cctgccagat acgagcctga
    37621 taatcgcgaa tagagcgatc cgagctaaac ataccgcagc gggcggtatt gaggatcgcc
    37681 gcgcgagtcc aggcctcctg gtcgcggtac agcacatcca cctgcttttg tgcctctaca
    37741 taggctgcga aatccgccat caccagatac ggatcgccgc cctgtttgcc gatactgtgc
    37801 agcatctggt cgaaggcatg cttatcgccg tcgctgtatt taccgctttc cagctctttc
    37861 aatactgcgt ccagcacctt atctttcttc cgccatttca ccgggtcgta gcctttggcc
    37921 agaattgcct tcacttgttc cacggtatga ccaaaaataa agatattttc ttcaccgact
    37981 ttctcggcga tttcaacgtt cgccccatcc agcgtaccga cagtaagcgc accattgagc
    38041 gccagtttca tattgccggt accggaagct tctttacctg cagtcgaaat ttgttcggag
    38101 atatccgccg ccgggatcag tttttccgcc gccgaaacgc aataatccgg caggaacacc
    38161 accttcaact tatcgccaac cagcggatcg ttgttgatca cgtcagccac tttgttgatc
    38221 gcaaagataa tattcttcgc caggtagtag cccggtgccg ctttcgcgcc gaagaggaag
    38281 acgcgcggta cgcgatcagc ctgcgggttt tcacgaattt ctttgtacaa cgccagaata
    38341 tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa
    38401 atcgcctgtg gattgatctc aataccggta cgaactttca caaactccgc cagacggact
    38461 ttattcgcct gcttgatctc gcgatattgc tgacggaatt tcgcatcatc agcgaatttt
    38521 tccagattga tcagctgatc gagatcgtta gcccactctt ttttcagtga tttatccaac
    38581 agagccgcca gtgccgggtt gcactgtttg atccagcgac gtggggtaat accgttggtg
    38641 acgttatgga atttgttcgg ccatagctgg tgatattccg ggaacagatc tttcaccacc
    38701 agatccgagt gcagcgccgc aacaccgttc accgcgaaac cgccaaccac acacaggttc
    38761 gccatatgca cttgtttgtc gtgcaccacc gccagtttgg cccacacttt ttcatcgccc
    38821 ggccaggttt tctccaccag cgttttaaag cgagtattaa tttcgttaat aatctgcatg
    38881 tggcgcggca gtaagccttt caccagtttc acatcccagc gttccagcgc ttctggcatc
    38941 agggtatggt tggtgtaagc gaaagttttg ctggtaatgg cccaggcgtc atcccagctc
    39001 atctggtgct catcgatcag cacgcgcagc agttctggaa tcgcgatagt tgggtgggta
    39061 tcgttcagct gaataacttc gtaatccgcc agttcgtgca gtttacgccc cgccagatga
    39121 tggcgacgca aaatatccgc taccgaacag gcacactgga agtactgctg catcaagcgc
    39181 agctttttac cggcagtatg gttgtcgttt ggatagagaa ctttggtcag tttttccgca
    39241 ttgatgccct gctgttcggc acgcaggaaa tcaccgtcgt taaatttagt cagatcaaac
    39301 ggatgcgcgt gcgtcgcctg ccacagacgc agcggctgcg ccacgccatt acgatagccg
    39361 acaacgggga gatcccacgc ttgaccggta atggtaaact ccggctccca gcgtccgtct
    39421 ttcgtcactt taccgccaat ccctacctgc acatccagtg cttcgttgtg gcggaaccac
    39481 gggtagttac tgcgatgcca gtcatccggc gcttcaacct gtttgccatc gacaaaagac
    39541 tggcggaaca aaccatattg atagttcaga ccgtaacccg tcgcagactg accgacagtt
    39601 gccattgaat cgaggaagca cgccgccaga cgtcccagac caccgttacc cagcgccggg
    39661 tcgatctctt cttccagcag gtcagtcaga ttgatgtcat aagccttcaa cgaatcctgt
    39721 acatcctgat accagccgag attcaacagg ttgttgcccg tcagacgacc aatcaaaaac
    39781 tccattgaga tgtagttaac atgtcgctga ttcgccaccg gcttggcgaa tggctgagca
    39841 cgcagcattt cggccagtgc ttcactcact gccagccacc actggcgagg agtcatttca
    39901 gccgcagaat ttaagccata acgctgccac tgacgtgaaa gcgcttcctg aaattgctta
    39961 tcgttaaaaa taggttgtga cataggagtt ccacttttct tagattttca acacaacgtt
    40021 atcgctagtt tgccaggctc gatgttgacc ttcctcatcc tgcgggggat taggcaggga
    40081 ggagttgcgg ggatgagcaa ggaaatgtga tctcaaccac ttaaagctag tgcaaaccac
    40141 aggattagca gcaaatcaat gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat
    40201 ggccgttgcg tattttcaaa aagcggaagg taactctata aattaagtaa aggagtgaaa
    40261 cagtttcaca agtaaaatat ccagtgtgct ccatctcatt cttaatagat ttattaagat
    40321 catcttttta gatagcactt tcatcaggaa tgaagaagaa acccttgctt aaatgaatct
    40381 gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg tgagttgtta
    40441 ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac gtcatcgctt
    40501 gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca aaaaaaataa
    40561 tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa aattaagtcg
    40621 tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc
    40681 gaacaacttc cggctggcgc tgatcacaag tcctgcgggc tacggtaaga ccacgctcat
    40741 ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggacg aaggtgataa
    40801 ccagcaagag cgtttcgcca gctatctcat tgccgccgtg caacaggcaa ccaacggtca
    40861 ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat gccagcctga cgtcactctt
    40921 cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg tcatcgatga
    40981 ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta ttcgccatca
    41041 accagaaaat ctcaccctgg tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa
    41101 tctgcgtgtt cgtgatcaac tgctggaaat tggcagtcag caactggcat ttacccatca
    41161 ggaagcgaag cagttttttg attgccgtct gtcatcgccg attgaagccg cagaaagcag
    41221 tcggatttgc gatgacgttt ccggttgggc gacggcacta cagctaatcg ccctctccgc
    41281 ccggcagaat acccactcag cccataagtc ggcacgccgc ctggcgggaa tcaatgccag
    41341 ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg caacgcgcca
    41401 ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gcactcatca cccgtgtgac
    41461 cggcgaagaa aacgggcaaa tgcgcctcga agagattgag cgtcaggggc tgtttttaca
    41521 gcggatggat gataccggcg agtggttctg ctatcacccg ctgtttggta acttcttgcg
    41581 ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg ccgccgcaga
    41641 aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg cggcaggcga
    41701 tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg agtctgttca accatagcga
    41761 actgtcgctg ctggaagagt cccttaaggc cctgccgtgg gacagcttgc tggaaaatcc
    41821 gcagttggtg ttattgcagg cgtggctgat gcaaagccaa catcgctacg gcgaagttaa
    41881 caccctgcta gcccgtgctg aacatgaaat caaggacatc agagaaggca ccatgcacgc
    41941 agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg atgaagcgga
    42001 acggctggca aaactggcac tggaagagct gccgccgggc tggttctata gccgcattgt
    42061 ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc gaattgaccc gctcactggc
    42121 gctaatgcag caaaccgaac agatggcacg ccagcacgac gtctggcact acgccttatg
    42181 gagtttaatc cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga
    42241 aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat ctggaacagt tgccgatgca
    42301 tgagtttctg gtgcgcatcc gagcacaact gttatgggcc tgggcacggc tggatgaagc
    42361 cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct tatcagccac agcaacagct
    42421 tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag ataacgcccg
    42481 tagccagctg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca gcgactggat
    42541 ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg accggcgata aagccgccgc
    42601 tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact tcctgcaagg
    42661 tcaatggcgc aacattgccc gtgcacaaat cttgctgggc gagtttgaac ctgcggaaat
    42721 tgtcctcgaa gaactcaatg aaaatgcccg cagtttgcgg ttgatgagcg atctcaaccg
    42781 taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg acgcccagcg
    42841 tgtgttgctg gacgcattaa aactggcgaa tcgcaccgga tttatcagtc attttgtcat
    42901 cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt caactcaata cgctgccgga
    42961 actggaacag catcgcgcac agcgtattct gcgagaaatc aatcaacatc atcggcataa
    43021 attcgcccat ttcgatgaga atttcgttga acgtctgcta aatcatccag aagtgccaga
    43081 actgatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc tgatttactc
    43141 cggttacagc aacgaacaaa tagcgggtga actggaagtg gcggcaacca ccatcaagac
    43201 gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca
    43261 cgcgcagaaa ctgctgaaga tgatgggtta tggagtgtga gtttagccgg ataatgcatt
    43321 ggatccggct tacatcttta tatcattcaa tactcacccg cgttacgcca tccgtttcta
    43381 tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc gatattggtc agcagatggc
    43441 atgagggatg ggcgaccgta aattgtcccg cgcccgccag cgccattggc aacactagct
    43501 ggtcagcaag atattccccc actgccgccg ggcttgccag gtagcgtttc acctctttca
    43561 ccaactgtgc cgcaaccacc tcggcactga cgcgcttttc accgacgaca aaaaagcgtt
    43621 cggtgatatt ttcactttcg acttcaagcg agacggtatt acccggcccc tggtcgcgcg
    43681 gcaggttatg aatatactgc tcatgcaggg gaaaactccc cgccagtgta gcgatttcac
    43741 gctcagcaac atggcgcggc acaccagcca gtagcacctc tccacgcatc tgcacaatgt
    43801 tcccgcgctc gccaagttgc aaggttttaa acgatgccac tggcgagact tctgttgcca
    43861 ccacgccgcc tccggcaggg taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc
    43921 ctattttcgc cagcagcggc tccaacaccc ggcggataaa atccgcaggc ggggccgacg
    43981 ggttatcggt accgccgctc acttcaacac gcgaaggtcc atcggcaaac cacagcgcag
    44041 gcagcaccgt ttgcagcacc agcgtacaac ttcctgcgct accgatagca aagcggtaat
    44101 cgccgccacg cacggtgccg ggccggaaga ccagacgctg cgaccccagc tccgcgcctt
    44161 ccaccgttgc cctacaaatt tccgcagctg cttttaccgc ggtcagatgc tggcgcaaca
    44221 gtcccggttt tgcccgcccg gcacgaatgc cggtgatggt aaatggctga ccggttatca
    44281 tcgacagact cagcgccgag cgcaggatct gcccgccacc ttcgccctgt gcgccatcca
    44341 gcgcaatcat ccttttcatc atttatcctt ttacgcacac cacctgacgc agggtataga
    44401 taacttccac cagatcgctt tgtgccgcca tcaccgcatc aatatcttta tacgccatcg
    44461 ggatttcgtc gatcacttcg gcatctttac ggcattccac atgcgcggtg gcacgaattt
    44521 gatcttccac gctgaacatt tttttcgctt tagtacggct cataacccgc ccggcaccgt
    44581 ggctgcacga acagaacgac tcttcatttc ccagcccacg gacgataaag cttttcgctc
    44641 ccatcgaccc tggaataatt ccatattgac cagcacgcgc agacaccgcg cctttacgcg
    44701 tcacgtagat ctcttcgcca aagtgctgtt ctttttgcac atagttgtgg tgacagttga
    44761 tctcttccat tgccagcgtt tgtggctgtc ttaccgtttt ctgcgtaatg ctctgcaacg
    44821 ccgttaccac gttttccatc atcgcatcgc ggttaaggct ggcaaaaagc tgcgcccagg
    44881 ccaccgcttt caggtaatca tcaaagtatt ccgtaccttc cataaagtac gccagatcac
    44941 gcgacggcaa cgtttcaagc tggtcctgca tctctttttg cgccagatcg ataaaataag
    45001 ttccgatggc gttaccaatt ccgcgtgaac cggagtgcag cataatccac acctgatccg
    45061 acccatcaag gcagatttca ataaagtggt tacccgttcc cagcgttccc aggtgtttat
    45121 agttgttagt gttaaggaaa cgcggatatt tttgcgttaa ccactgataa ccggcctcca
    45181 gctcagccca tttcgcatcg acgttaacag gcgggttttc ccatgcgcct ttatcacgtt
    45241 tacaacggcc agtggtacgc ccgtgcggca ccgcagtttc aatcgcctga cgcagctccg
    45301 ccaggttttc cggcaggtca gccgccgtta aagcggtacg tagtgcgttc attccacaac
    45361 cgatatccac gcccaccgcc gccggaataa tcgccccttt ggtcgggatc acgctaccaa
    45421 tggtggaacc tttacccagg tgtacatcag gcattaccgc aatatgtttg aaaataaacg
    45481 gcatcttcgc cgtattaata agttgctgac gcgcatcggc ttctaccggc acgcctttgg
    45541 tccacatttt taccgaggca ttttcagtgg tcagtaattc gtaattcatt ttgtttttct
    45601 cttttcgttg gtatgtcctg ataattgcaa ccgtcgtgcc agaaaattaa ataagcagat
    45661 taatttttta attcattgtt ttaaaaaaga ttatatcttt acgtccgtaa ccggagattt
    45721 cccgcaaagc cgatttaccg ataatgaaat atcgtctttt ataaggatat ctaagatgcg
    45781 taaaacagtg gcttttggct ttgtcggcac cgtactggat tatgccggac gcggcagtca
    45841 gcgctggtcg aaatggcgtc cgtcactctg tatatgccag caagaatcgt tagtcatcga
    45901 tcgactggaa ttattgcacg acgcccgctc gcgctcgctg tttgaaacgc ttaaacgcga
    45961 tatcgtcagc gtttcgccag aaacagaagt ggtgagcgtt gagattgagc tgcataaccc
    46021 gtgggatttc gaagaggttt acgcctgcct gcatgatttc gcccgtggtt acgagtttca
    46081 gccagaaaaa gaagactatt taattcacat caccaccggc acccacgtcg cgcagatttg
    46141 ctggtttctg ctggcggaag cgcgttacct gcccgcccgg ttgatacaat cttcacctcc
    46201 gcgcaaaaaa gaacagccgc gcggcgcagg tgaagtgacg attatcgatc tcgatttaag
    46261 ccgttataac gccatcgcca gccgctttgc cgaggaacgc cagcaaacgc ttgattttct
    46321 taagtccggt attgccacgc gtaaccccca cttcaaccgc atgattgagc agattgaaaa
    46381 agtggcgatt aaatcccgcg caccgatcct gctaaacggc ccaaccggtg cgggcaagtc
    46441 atttctggcg cgacgtatat ttgaattaaa acaggcgcgg catcagttta gcggcgcgtt
    46501 tgtggaagtg aactgcgcaa ccctgcgcgg cgataccgcc atgtcgacgc tgtttggtca
    46561 tgtgaaaggc gcgtttaccg gggcgcggga atctcgtgaa ggattattac gcagcgccaa
    46621 cggcggaatg ttgtttcttg atgagattgg cgaactgggc gcagacgaac aggcaatgct
    46681 gcttaaagcc attgaagaaa aaacctttta tccgtttggc agcgatcgcc aggtgagcag
    46741 tgattttcag cttatcgccg gaacggtgcg tgatttgcgc cagctggttg ccgaaggaaa
    46801 atttcgcgaa gacctgtacg cgcggatcaa tctctggacc ttcaccctgc cggggctacg
    46861 ccagcgtcag gaagatattg aacctaacct ggattatgaa gtggagcgcc acgcttcact
    46921 caccggcgac agcgtgcgtt ttaacaccga agcgcggcgc gcctggctgg cctttgcgac
    46981 atctccccag gcaacatggc gcggtaactt tcgcgagctt tctgccagcg tcacgcggat
    47041 ggctaccttt gccactagcg gacgcatcac tctggaagtg gtggaagatg agataaaccg
    47101 tctgcgctat aactggcagg agagtcgccc ctccgcgctt acgcagctac tgggcgcaga
    47161 ggcagaaaac atcgatctct tcgaccgtat gcaactggaa cacgttatcg ctatctgccg
    47221 ccaggcaaag tcactttctg cagccggacg acagcttttt gatgtttcgc gccagggcaa
    47281 agccagcgtc aacgacgcgg atcggctacg caaatacctg gcgcgttttg gtctgacgtg
    47341 ggaagcattg caggatcagc acagctccag ttgaatatgg tggtccgtca gcacctgcat
    47401 cacgcttact ggcggcgggg catcggtgta gacggcatct accatgctga tgctgcccat
    47461 attgaccatc gcgttacggc caaatttcga gtgatcgaca accagcataa cgtggcgcga
    47521 gttctcaata atggcgcgtt tggtgcgaac ttcgtggtaa tcgaactcca gcagcgagcc
    47581 gtcgctatcg atgccgctta tccccagaat gccgaaatca aggcggaact gggagataaa
    47641 atcgagcgtc gcttcgccaa tgatcccgcc atcgcggctg cgtaattcgc caccggcgag
    47701 aataatacgg aaatcctctt ttaccatcag cgtgttagca acgttaagat tgttggtgac
    47761 aatgcgcaaa ttgctgtgat taagcagcgc gtgcgccacc gcttccggcg tggtgccgat
    47821 atcgataaac agcgtcgagc cgttggggat ttgctccgcc actttacggg cgatgcgctc
    47881 tttttcttcg gtctgggtgg ctttacgatc gtgccacggc gtgttaaccg aactggaagg
    47941 cagtgccgca ccgccatgat ggcgcagaat caggttttgc tccgccagct cattgagatc
    48001 gcggcgaata gtctgcgggc tgacggagaa gtgctccacc agctcctcgg tgctgacata
    48061 accctgctgt ttaaccagtt cgataatacc gttgtgacgt tgtgtttgtt tcatttataa
    48121 atccctggaa ttattttcgt tttcgcgcat tgagcgaatc aacaaaagcc atcgctaaac
    48181 ccacggctaa cccggcgatg tgtgctccgt tcgccatcga catcccaaac aaatcaaacc
    48241 atccggcgac aatccagatc agcgcaaaga taattaaccc acgttgcagg taaatgccac
    48301 tttgcggatc gcgttcgcca cgtagccaga cgtagcccat cagcgcatac accacgccag
    48361 aaagcccgcc aaaccacggc ccgctgaatt tttgctgcac atagccgctt aacagggcgc
    48421 taatgagagt aataacaatt agcttaccgc taccgaggcg tttttccacc gcgccgccga
    48481 gataccacca ccagagcagg ttaaagagga tatgcatcag cgagaagtgc attaacgcgt
    48541 gggtgaagta acgccagaac tcaaatttca gtgctggatc gaatggccag gccagccata
    48601 acatcacttc ctgatcgccg agaatttgca tggcaataaa caccaccacg caggcgatca
    48661 tcatcaccca ggttaccgga cctgcgcgtt cacgcaaggc ggcaaagaaa ggataacggc
    48721 gataatgcag gccactgccg gtatggcctg cctgccagct cgccgccaga taacgcggat
    48781 ctgccgggtt ttcgagaaaa cgcgccagct ccgcccgtac gcgctcggcc tgggactcat
    48841 ccgccagcca gacatcgctt tggttatgtt gttgaatcgt gaggataaca ccctgcgtcg
    48901 ccatgtaatc aacaaacgcc tgcgccacgc gggggttagc aaaagaggta atcatcaaca
    48961 tcgttgctgt cgcttattcc acacaaaagg ggacagtata aagcgttacg cgccgtacgc
    49021 cacctctgcg ggaaactgac gttgccaggc ttcaaagccg ccgtcaatgc tatagaccac
    49081 atcgtagccc tgttgcagca gatactgcgc cgcgcctttg ctgctattgc cgtgataaca
    49141 catcaccatc accggagtgt caaagtcgtt attacgcata aaagcgccca gcgtgtcgtt
    49201 ggttaaatgg aaagcctgca ccgcatgtcc cattgcgaaa ctctgtggat cgcgaatatc
    49261 gaccagcacc gcctcttttt cctgcaactt ctggtgcgcg tcggcaacgt taatacattc
    49321 gaactgatcc atgcgtctct ctttctttac aaacaagtgg gcaaatttac cgcacagttt
    49381 acgtcgaagc ggcagataaa cgccataatg ttatacatat cactctaaaa tgttttttca
    49441 atgttaccta aagcgctatt ctttgctaat atgttcgata acgaacattt atgagcttta
    49501 acgaaagtga atgagggcag catggaaacc aaagatctga ttgtgatagg gggcggcatc
    49561 aatggtgctg gtatcgcggc agacgccgct ggacgcggtt tatccgtgct gatgctggag
    49621 gcgcaggatc tcgcttgcgc gacctcttcc gccagttcaa aactcattca cggtggcctg
    49681 cgctaccttg agcactatga attccgcctg gtcagcgagg cgctggctga acgtgaagtg
    49741 ctgctgaaaa tggccccgca tatcgccttc ccgatgcgtt ttcgcctgcc acatcgtccg
    49801 catctacgcc cggcgtggat gattcgcatt ggtctgttta tgtacgatca tctgggtaaa
    49861 cgcaccagcc tgccgggatc aactggtttg cgttttggcg caaattcagt gttaaaaccg
    49921 gaaattaagc gcggattcga atattccgac tgttgggtag acgatgcccg tctggtgctc
    49981 gccaacgccc agatggtagt gcgtaaaggc ggcgaagtgc ttactcgtac tcgtgctact
    50041 tctgctcgtc gcgaaaacgg cctgtggatt gtggaagcgg aagatatcga taccggcaaa
    50101 aaatatagct ggcaagcgcg cggcctggtt aacgccaccg gcccgtgggt gaaacagttc
    50161 ttcgacgacg ggatgcatct gccttcgcct tatggcattc gcctgatcaa aggcagccat
    50221 attgtggtgc cgcgcgtgca tacccaaaag caggcctaca ttctgcaaaa cgaagataaa
    50281 cgtattgtgt tcgtgatccc gtggatggac gagttttcca tcatcggcac caccgatgtg
    50341 gaatacaaag gcgatccgaa agcggtgaag attgaagaga gtgaaatcaa ttacctgctg
    50401 aatgtgtata acgcgcactt taaaaagcag ttaagccgtg acgatatcgt ctggacctac
    50461 tccggtgtgc gaccactatg tgatgatgag tccgactcgc cgcaggctat tacccgtgat
    50521 tacacccttg atattcatga tgaaaatggc aaagcaccgc tgctgtcggt attcggcggt
    50581 aagctgacca cctaccgaaa actggcggaa catgcgctgg aaaaactaac gccgtattat
    50641 cagggtattg gcccggcatg gacgaaagag agcgtgctac cgggtggcgc catcgagggc
    50701 gaccgcgacg attatgccgc tcgcttacgc cgccgttatc cgtttctgac cgaatcgctg
    50761 gcacgtcatt tcgcgcgtac ttatggcagc aacagcgagc tgctgctcgg caatgcggga
    50821 gcggtaagcg atcttgggga agatttcggt catgagttct acgaagcgga gctgaaatat
    50881 ctggtcgacc acgagtgggt ccgccgcgcc gacgatgccc tgtggcgtcg cacaaaacaa
    50941 ggtatgtggc taagtgcgga acaacaatct cgtgtgagtc agtggttggt ggagtatacg
    51001 cagcagaagc tgtcgctggc gtcgtaaatt aacgtaaggt gatcaggtca gatttcaatc
    51061 tggcctgaga ctgatgacaa acacaaaatt gcctgatgcg ctacgcttat caggcctacg
    51121 tggtttatgc aatatattga atttgcatga ttttgtaggc ctgagaaggc attcacgccg
    51181 catcaggcat gaacaaagtg ccacgttgtc aacaatctga cctacttttt gattcaggga
    51241 attaccgcaa agcccacgcc atcataccca acgtaacaaa acttaagcac gttccccact
    51301 cactccactt ccgtatctgg catctatttt tgctgcgcca ctggcaataa tatcccaacg
    51361 caaaaccaag ctgtgcgctt attggaccga taaaatccgt ccacggctga ggccaaaatg
    51421 gcgaaaaatt caggggtagc cagacaacaa gcagaaagaa attgcggata ctttccagca
    51481 acgcaatttt cgctggcgcg atgtaaaaaa tgatattcca gaggatcaac cataaataga
    51541 ccggtgcgaa taccaaccaa caatggagcc tgcttttcaa ttcagattga gttctacgag
    51601 taaattgcca accaacgcaa cacattacac agaagaaaag cgcatattca gccgataacc
    51661 aaagttgtaa ttccagaagg gaaatacgcc tgtgcatgaa cgaatttgag aacagcatct
    51721 ccattagaaa actcagtgtg ataccaacaa caaccaccgt cggccacacc aacaacggca
    51781 caataacaaa gccgagatat agcggataaa tataaagtct gattttattc atctttattg
    51841 tcctgattat tcgggtgatg cgtcatacat cgcgcataaa cactcatcaa tcatccagtc
    51901 gtcttcccag acttgatatt ctgtcattgg ttcaggctgt tttagtgggt tctcaatcgc
    51961 ttgtcgcgct ctttctttca gcaaaggatg attatttcta gcccaggtat taatttgagt
    52021 ggtaatagtt ttattatcat cactctgctc aaaatcgcta ttttcgacgt attctttact
    52081 gttacgccag agcaaacatg ccatagacgc cgtcggccag taaccggaaa tggacataat
    52141 ggcgtcaagt aagggttcaa tctttatacc accgcgataa aacatttcta atattatttc
    52201 cagttccacg acctccttag ccgacaccca atcctgcata tataaatagg cgaaatcgct
    52261 aattatttgt agttcatccg ttcgccagtt ctggttatct gcctctgcta ttctctgtaa
    52321 acagcagcct tcataaatgc ctggaaattg cctttgggca ataaattcaa acaatctggg
    52381 taataaatag cgaatctcat catcattttg cggggtagga ccaatacttg ttccccaggc
    52441 atgtaataaa gggaaaggta tggcacgcag tgaaacccct cttaaggctt gttgttgttc
    52501 ttcgctaaag cagtaaggac aacaaacatg aaatctcgac ggcaccggat aacgacgaaa
    52561 aatatgataa atctgctcaa ttaatgcctg catggcgggg ctgcattgca taattactcc
    52621 ctcttccgct gattgcgtgt aaacggttga taaacaaaac gagcattggc cgcgttcagg
    52681 cgaataatac cggtatccat gtgattatcc tgcttaaggt aaggttttat acatcggcac
    52741 ataacctaca tcttccacca aactctgccc ctgcggcgtt aaaaaccatt ccaccaattt
    52801 ttgtgtctct gacgtcgtat tttccctcgt taccataaag gcatcgatga tatagggata
    52861 tttgccattg cgaatatttt ccgcagtcgg tgcaataccg ttaatcgcta gcaattttat
    52921 atttttatca gcattcattt gcgtcgcgta atagcggaag gtatagccta ttgcgttgtt
    52981 tgtattacgg tattcggcaa cgactttaat cattccctcc atcacgcttg ccacttccgt
    53041 ttcctgcggc gagatcatgc ggacattttt catgacctgt gattgcatca ctgtctggct
    53101 gccagagtct tccgggcgct gccaggtctg gatctcctga tcgttaccgc caacagtacg
    53161 ccagttggta attgcaccac tgaaaatgtc acgcacctgt tgttcagtca tggaattaac
    53221 cggattatcc gcattgacga tgaaaacgaa tgcttcacgg gcaaaggggg tgtacagcaa
    53281 agtgacacca gattcctttg cacgtttttt ttgcccaccg gaaggttgcg ccacgaaaat
    53341 aatatcagca tcgcccttaa cgattttgtt atatgcttct ggcgtacgag agttttccag
    53401 ataatcccaa acgtgaaaat cctctggtat tacacttaat gcataaaatg cagaagcata
    53461 aattgggtac gcagccgtgg cgccatcgat tcgcggccag ttttgcctaa actgaatttg
    53521 cggtttgcca cgcagcggcg tcagtcgatt attgagttta tcgggttgcc aggcccagat
    53581 atcaatattt tcgcgtacac cgacaccagg attgagctgt ttatataaat aaccctgata
    53641 accagcgaca aaaccaagca tgagaaggat aaacaggagg gaattacggc aacgtagccc
    53701 ggatgtaccg gttaaaggat gtctggttgg ccggccgtac cccagcgaaa aactgatatg
    53761 cgcgccaacg ggtataatga ctatccacca gtactcacct ccgactactg agactaacaa
    53821 aacgcccagc caggggagca taagatcagc caaccaaccc gatcccatct ggtgaaattg
    53881 ataatctgcc agatgcagcg cgattatcca gccaaccagg gttaatagcg cgggtaagag
    53941 aaaaaagcat tgccgccaga cggctatttc tctgttttct ggcgcgctat caccggccca
    54001 cacgcgacca gagaaaaata ttattatcgc ccccagcgtc agcataaaga cgttgagtag
    54061 aagataaatt gttggcgtga taaggatatt acaaacttca ataataccgg taagcccgac
    54121 acccagcatg gcaacaaccg ctgccaaaaa ttgcgccagt atggggatgc cgaaaaaagt
    54181 cattatcagc gaggtcaaaa tccattttct atttggcatc attctgtcca ttcttcctga
    54241 atgatggaat tatactcccc gagaaccctc gccccctctg gacacttttc cgaaatgatg
    54301 gcgaaaaaaa cgggaccctt tggccccgtt ctatttattg gtgaaattac aatctcaccg
    54361 gatcgatatg ccagatatga tcagcgtact ctttgatagt acggtcagaa gagaagtagc
    54421 ccatattggc aatgttcagc atcgcttttg cggtccactc ttcctgaagc tcgtaaagtt
    54481 cgtcaacttt atcctgacaa tcgacatagc tgcgataatc cgccagtacc tggtagtgat
    54541 cgccgaagtt gatcagcgaa tcaaccagat cgcgatagcg acccggatct tccggactga
    54601 atacaccgct gccgatttgc gtcagcacct gatgcagctc ctcatctttc tcatagtatt
    54661 cacgcggttt gtagccctga cgacgcagtt cttccacttc ttccgctgtg ttaccaaaaa
    54721 taaagatatt gtcagcaccg acatgatcca gcatctcgac gtttgcaccg tccagcgtac
    54781 cgatagtcag cgcgccgtta agcgcaaact tcatgttact ggtgccggaa gcttccgtcc
    54841 ccgccagcga aatctgttca gacagatcgg ccgccggaat gatcaactgc gccaggctaa
    54901 cgctgtagtt cgggatgaac acgactttca gcttgtcgcc aatctgcgga tcgttgttga
    54961 tcactttcgc tacgtcattg atcaaatgga taatatgctt cgccatgtaa taggccgaag
    55021 ccgccttacc gccaaaaata ttcacgcgcg gtacccactt cgcatccggg tcggccttga
    55081 tgcggttata gcgggtaatc acatgcaaca cattcatcaa ttgacgtttg tattcgtgaa
    55141 tacgtttgat ttgtacatcg aacaacgcct taggattcac caccacattc agctgctggg
    55201 cgatatactc cgccagacgc tttttgttct ccagcttcgc ctgatgcaca gcgtgattaa
    55261 ccattgggaa atcacagtgt tgttgcagct cattaagcag gctaaggtcg gtgcgccagt
    55321 tacggcccag gtgttcgtcc agcacggctg aaagcgatgg gttcgctacc gccagccagc
    55381 gacgcggcgt cacaccgttg gtgacgttgg tgaaacgacc cgggaagatt ttcgcaaagt
    55441 cggcaaacaa cgattgcacc atcagattag agtgcagttc cgatacaccg ttaactttgt
    55501 ggctcacaac aaccgccagc caggccatac gcacacgacg accgttggat tcatcaatga
    55561 tcgacgcccg tcccagcaga tcggtatcgt tcggatactg ttcctgcaag gttttcagga
    55621 aatagtcgtt gatttcaaag atgatctgca ggtgacgcgg cagaatttta cccagcatat
    55681 caaccggcca ggtttccagc gcctcgctca tcagcgtgtg gttagtgtag gagaagacct
    55741 gacaacacac ctcaaacgcg tcgtcccagc taaattggtg ctcatcgatc agcagacgca
    55801 tcatctcagg aatcgacagt accggatggg tatcattgag atgaatcgcg attttatccg
    55861 ccaggttatc gtaggtttta tgcaactgat aatggcggct taaaatgtcc tgaatggtcg
    55921 aggaaaccag gaagtattcc tgacgcaggc gcagctcacg cccggagtag gtggagtcat
    55981 ccggatacag tacgcgagat acgttctcgg agtggttttt atcttccact gccgcgaagt
    56041 agtcaccctg gttgaattta ccgaggttaa tttcgctact ggcttgcgca ctccacaaac
    56101 gcagcgtgtt ggtcgcgtcg gtgtcgtaac cagggattat ctgatcgtaa gcgactccca
    56161 gaatctcttc ggtttcaatc cagcgcgttt ttttaccttc ctgctgaatg cgaccaccaa
    56221 aacggacttt atagcgcgtg ttgtggcgtt tgaattccca cgggttacca tattccagcc
    56281 agtagtcagg cgactctttc tggctaccgt taacgatgtt ctgcttgaac ataccgtagt
    56341 catagcggat gccgtaaccg cgccccggca accctaacgt cgccagagaa tcgaggaagc
    56401 aagccgccag acgtcccagg ccaccgttac cgaggcccgg gtcattttct tcatcaatca
    56461 gctcttcgag atttaacccc atcgcttcca gtgcgccctg tacatcttcg taaattccta
    56521 gcgacaacat ggcgttggag agcgtacggc caatcaaaaa ctccatcgac aggtagtaaa
    56581 cctgacgagt ttcttgcgac aactgggcac ggtttgaacg taaccagcgc tccacgagac
    56641 gatcgcgcac agcaaataac gttgcgttca gccattcatg tttattggcg acgaccgggt
    56701 cctttccaat cgtaaacatc agcttgtaag cgatagagtg cttaagagct tctacgctaa
    56761 gcgtgggcga tgaatatgta aacggagcat tcatataggc gtttcctgaa aactatttca
    56821 agcgatagta aagctcacgg tacgacttcg ccgcgacctg ccagctaaaa tccattgcca
    56881 tagcctgacg ttgcacaaac cgccacagtg aaggacggga ccacagtaca aaagcacgtc
    56941 gaatagcccg taacagcgac caggcattac tatcttcaaa gacaaaccca ctggcgacgc
    57001 catctgcaag gttctcaaga gaacagtcag aaaccgtatc agcaagccca ccggtgcgcc
    57061 gcactaacgg cagcgtaccg tacttcaatc cataaagttg cgttaagccg cacggttcaa
    57121 aacggctggg caccagaatg acgtccgcgc cgcccataat gcgatgcgaa aatgcttcgt
    57181 gatagccaat ctgaacgccc acttggccgg ggtattccgc tgccgccgca aggaaacctt
    57241 cctgcagcac cggatcgccc gcgccgagta gcgccagctg cccgccctgc tccagaaggc
    57301 ccggtaaggc ttccagcacc aggtcgagac ctttctggct ggtcagacgg ctcaccactg
    57361 caaaaagcgg cactttatcg tcaaccttaa gccccattgc gatttgtaac tggcgcttat
    57421 tttccgcttt atcttccaac gtatcgcggg tgtaacgcga ggccaacagt aagtccgtct
    57481 ctggactcca gattttctcg tccacgccgt tcagtacgcc ggaaagacgc ccttcacggt
    57541 gacgctgttg caacagacct tccataccgt aggcaaactg cggttcggtg atctcgcgag
    57601 cgtaggttgg actgaccgcc gtaatgtgat cggcatagta cagaccggcc ttcaggaaag
    57661 agatttgtcc gttgaattcc agcccatgaa tattaaagaa tgaccatggc aattggatgt
    57721 cattcatgtg atgtgcataa aacatgcctt gataggccag gttgtgcaca gtaaacaccg
    57781 acttcgccgg acgcccgcgc gccgccagat acgcaggcgc aaggcctgca tgccagtcgt
    57841 gcgcatgcac cacatcagga cgccagaatg ggtcaagccc gctggccatt tctgccccaa
    57901 cccaccccag cagcgcaaaa cgcaatacgt tgtcggtata ggcaaataag ttggtatcgt
    57961 gatacgggct tcccggacga tcatagagat gcggcgcgtc aatcaggtat atgccaaccc
    58021 cgttgtaatg accgaacaac agcgtgatat gcccggcaaa ggtatcacga cgggatacta
    58081 cctgcgcatc ggtcacgcca cggcgaatat cgggaaatgc aggcaacagt acgcgagcgt
    58141 caacgccgtc tgcgatttgt gctgcgggta atgccccaat aacatcagcc agaccgccgg
    58201 ttttaagcag cgggaacatc tctgaacata catgtaaaac ctgcattatc gctcctgttt
    58261 atgccctaac ttccgtagca tttcgcgcgt taccagcacg atgccttctt ctgaacgata
    58321 gaaacgacgt gcatcttcct ctgcgttttc accaatcacc atgccttccg gaataacaca
    58381 agcacgatcg atgacgcagc ggcgcagacg gcacgagcga cctacccata cttccggtaa
    58441 caatacggcg gaatcaatgt tgcagaatga attcacgcga acgcgcgaga acagaacgga
    58501 ctgcaccacc accgaaccgg agatcacaca accgccggaa accagtgagt taagggtcat
    58561 cccgtggcta ccggagcgat cctgcacgaa tttcgctggc ggtaatgatt cattgtaggt
    58621 gcgaattggc caattgcgat cgtacatatc cagctccggc accacagagg ccagatcgag
    58681 gttcgctttc cagtaagctt ccagcgtacc cacatcgcgc cagtacggct cggcatccgg
    58741 atcggattgt acgcaagaga gcgggaacgg gtgcgcatag gccagaccgg cttcggtgat
    58801 cttgggaatc aaatctttgc caaagtcgtg gctggagttc tcatcgcgat cgtcttcttc
    58861 cagcagttca tacagatagt cggcgtcaaa gacgtagata cccatactcg ccagagattt
    58921 gctcggatcg ttcggcattg acggtgggtt agcaggtttt tcaacgaatt cgataatttt
    58981 atcgttctca tcaaccgcca taacgccaaa tgcggaggct tcttcaatcg gtactggcat
    59041 acaagcaacg gtgcaacgtg cgcctttttc gacgtgatcg ataagcatac gcgagtagtc
    59101 ttgcttgtag atatggtcgc ccgccaggat caccacgtat tccgctttat aacggcgaat
    59161 aatatcgagg ttttgggtga ccgcatctgc ggtgccgcga taccagtttt cccctttcat
    59221 tctctgctgt gctggcagca gatcgacaaa ctcgttcatt tcttcattga agaatgacca
    59281 gccgcgctga atgtgctgca ccagagtgtg ggactggtac tgggtgatca cgcccatacg
    59341 acggatcccg gagttgatgc agttagacag cgcaaagtcg ataatgcgga acttaccgcc
    59401 gaagtgtacg gccggttttg ctcgcttatt ggttaaatcc ttcaggcggg taccacgtcc
    59461 tcccgccagt atcagggcaa cagatttcaa tggcagctgg cgcgccaaca ttaagtgatc
    59521 gttcttctct aaactaacca tgactaactc cttttttatc atctctggaa cacacacaat
    59581 ccgtgtgcag gtccctgcca gacagccgta atcactgggt tatcctctcc agcgaatggg
    59641 ggaatggcgt gccactcccc agcaggtaaa acaatctctg ttacctcaag cgtggcgtta
    59701 attgcgatca aaaagcgatc cgagagcaga atttgcagct gtttcggccc gttttgccac
    59761 tcatccgtgc ttaaaggttg agcatatcga tttagccaac ggacattgcc gtcgccttct
    59821 tcccaccagc gattctccac caaagcggga atacgcttgc gcagatggat taacgcggcg
    59881 gtaaatgcgg ttaaaccact gcttgcctgc gaccagtcca accaggttaa ttggttatcc
    59941 tgacagtagg cattgttatt gccatgctgg ctgtgaccat gttcgtcacc ggccagtaac
    60001 atcggcgtgc cctgggagag caacaacgtt gttaacaggg catgaatgct gtcgcgccgc
    60061 cgttcaacca gatcaagagt accgcctaac ccttctttac catgattgtt actgtaattg
    60121 ttgttggtcc cgtcgcgatt ttcttctccg tttgcttcat tgtgtttatg gttgaagcaa
    60181 acgcagtcgc gaagcgtaaa accgtcatgt gcggtgacga gattaatcgc ggcactcggc
    60241 agacgaccat tacgtttaaa aacatcgctg gaggcagcaa aacgcccggc aaacgccccc
    60301 agaggcaaat cataatgcag ccagaaacga cgggcagcat cgcggaaatg atcgttccac
    60361 tcggcaaaca gcggcgggaa atttcccacc tgataaccac caggagcgat atcccacggt
    60421 tcagcaatta acttcacctg cgagagcacc gggcagttct ggatagcggt aaacaacggc
    60481 gcatcctgac ggaactctgg cgtacggccc atgactgccg ccagatcaaa gcggaaacca
    60541 tcgacgtggc aggtttctac ccaataacgc aggcaggcgc tggcataatc caccaccgcc
    60601 gggtgactca aattgagcgt gttgccacaa ccggtccagt tgtgataatc gccgtcttct
    60661 cttatccaat aatagctacg gttgtcgatc ccacgcagcg agaataacgg gccgtcgagg
    60721 tccagttccg cactatggtt gagcacgata tcaagaatga cttcgatacc cgctttatgc
    60781 agtgctttga ttgcatcgcg aaactcatgc agcgccgttt ctggcgagca ggcatacgcc
    60841 ggatgcagcg caaacatcgc caccgggttg taaccccagt agttacttag ccccatgcgt
    60901 tgcagacgtg gttcactggc aaactgcgcc actggcagca gttccagcgc ggtaatgccc
    60961 aactgtttca aatagttgat catcaccgga tgcccgaggg ctttataagt gccacggatc
    61021 tcgaccggga tctccgggtg caggtacgtt aatcctttaa catgggcttc ataaatgatg
    61081 gtgctgcccc acggcgtgcg cggcggggca tcatcttccc agtcatagtg atcaaccacc
    61141 actacgcatt tcggcgcaat ggcggcgttg tcgcgatagt caggttcatt atgaccggcg
    61201 tgcagcagcg ggttatcttt aaactccccg tcaatttgcc gcgcgcaagg atcaatcaac
    61261 aacttcgccg ggttaaagcg atgcccctcg gcgggttgcc aggggccatg aacgcgataa
    61321 ccataacgca aacccgggcg cgcatccggc agataaccgt gccaaatgtc gccactgtgc
    61381 cctggcaagt catagcgatg ttcctggcca ttggtgtcaa agacacacag ctctacccgc
    61441 tcggcatgag cggagaaaag tgtgaagttg acgccctgac cgtcgtaatg cgcgccgagg
    61501 ggagcgggtt tgccaatggc gagttgtgtc attctgcctc ccgaaccagc cagatagtgg
    61561 ccagcggtgg tagcgtcagg cttagtgaat gctgacgacc atggctggca atctcatcgc
    61621 tgtgtaccgt gccgccattg cctgcattac tgccgtgata gtgcatggaa tcggtattga
    61681 ggatctcacg ccatttgcct ggctgattaa tgccgaagcg ataatcatga cgcggcaccg
    61741 gcgtaaagtt actggcaacg atgatttcgt taccctcttt atcgcgacgc acaaagatca
    61801 gcaccgagcg ttctttgtca tccaccacca gccattcaaa gccgtacggg tcaaaatcca
    61861 gttcatgcat cgctttatgg tggcggtagg tgaggttcag atcgcgcacc agacgctgga
    61921 caccgtggtg ccagttatcg ccgccttcca acagatgcca gtcgaggctg gcgtcatggt
    61981 tccactcgcg gccctgggca aattcgttcc ccatgaacag cagtttcttg cccggaaatg
    62041 cccacatcca gccatagtaa gcgcgcaggt tagcgaattt ctgccatgcg tcgcccggca
    62101 tgcggtcgag aatcgatttt ttaccgtgga ccacttcatc atgcgacaac ggcaggacga
    62161 agttttcagt gtagttgtag agcatcccga aggtcagttt atcgtgatga tactgacgat
    62221 aaatcgggtc gagcttcatg tagtccaggg tgtcatgcat ccagccgagg ttccacttgt
    62281 accagaagcc cagaccgccc atatcctgcg gacgagaaac gccagggaaa tcggtagact
    62341 cctcagccat tgtcaccgca ccggaaacct gctcaccaag aatacggttg gtattacgca
    62401 agaattcaat cgcttcaaga ttctcgcgcc cgccaaattc gttcgggatc cactccccct
    62461 ctttacggct gtagtcacga taaatcatcg acgccaccgc atcgacgcgc agcgcatcaa
    62521 taccaaaacg ttcaatccag taaagcgcgt taccgacgag gaagttactg acttcacggc
    62581 gaccatagtt gtaaatcagc gtgttccagt cctgatgata gccttcgcgc ggatcgctgt
    62641 gttcatacaa gttcgtacca tcaaattcgg caagcgcaaa gtcatcagtc gggaagtggc
    62701 ctggcaccca gtcgagaatc acgttcagac cagctgcgtg tgcagcatca atgaaataac
    62761 ggaagtcgtc gcgagtacca aaacggcggg ttggtgcata caggccggtt ggctgataac
    62821 cccaactgcc atcgaaggga tgctcgttaa tgggcagtag ttcgaggtgg gtaaagccca
    62881 tccatttagc ataaggcacc agttgatcgg ccagctcgcg gtagctcaac cagaaattgt
    62941 tgtcggtgtg gcgacgccag gagcccaggt gaacttcata tatagagatt ggcgcatcaa
    63001 actgattcgc ttttttgcgc tcttcagtct gtacaacctt ttccggcagc ccgcaaataa
    63061 gagacgcggt ttccgggcgc atttgcgctt cgaaggcata agggtcggac ttcagacgca
    63121 agttgccatt ggcatcaatc atctcgtatt tatagagctg accgttatgc gccccaggga
    63181 taaacagttc ccagatgccg ctctctttac gcaggcgcat cgggtgacgg cgaccgtccc
    63241 agtagttgaa ttgcccaacc accgagaccc gacgggcgtt tggagcccag acagagaaac
    63301 gcgtacctgt gacgccatcc atagtatctg catgcgcgcc taaagtttca tacgggcgca
    63361 ggtgagtacc ttcagataat agccaggcat ccatttcctg gattagcgga ccaaaacggt
    63421 aaggatcatc aatcaggttt tgctgcccat gccagacaac agccaactga tagcggaaaa
    63481 aattcttacg tcgcggaatg acgccactaa agaatccccg tgagtcgaga cactccagtt
    63541 ttgcgagttt gcgcccggtt ttcggttcaa tcacccacac atcggtagcg tcgggtaaaa
    63601 gggcacggac ttccagtccc gcggtggttt tatgcattcc cagtacggaa aaaggatccg
    63661 caaaatggcc tgcaattagc gcgttaatca cgtctctatc gatacgatcg gacatgcttg
    63721 tcttcctgtt ttattgtgtc accccatcca attggatttt tgacttctgg ttgtgacatt
    63781 tttttgacct gaacggcgca gcactctgtg catcctctct gcgtcgtcct cacttcaggt
    63841 aaggctgtga atactcatgt attccgccac ccttaaagaa tagccaatgc tctatttaac
    63901 tcccggtaaa tcatgaaaca tctgcgctta ctcctgtatt acgcactaac aggggcggca
    63961 tcgcgcccca gatttaatga ataaagatta cgccagttga cgaagcatcc gacgcagcgg
    64021 ctccgcggcc ccccacagca gctggtcgcc cacggtaaag gctgacagga actctggtcc
    64081 catattcagc ttacgcaggc ggcctaccgg cgtggtcagc gtgccggtaa cggcagctgg
    64141 ggttagctca cgcatagtga tttcccgatc gttcggaacg actttcgccc acggattgtg
    64201 cgcagccagc agttcttcca cggtcggaat cgacacatct tttttcaatt taatagtgaa
    64261 tgcctggctg tggcagcgca atgccccgac acgcacacat aaaccatcta ccggaattac
    64321 ggaagatgtg ttgaggatct tgttggtttc cgcctgccct ttccactctt cgcggctctg
    64381 accgttatcg agctgtttgt cgatccacgg aatcaggcta cccgccagcg gcacgccaaa
    64441 gttatccacc ggcagctcac cgctacgggt taaggttgtg actttgcgtt cgatatcgag
    64501 aatagcagag gacggggtcg cgagttcatc tgctacatgg ccatacagat ggcccatctg
    64561 ggttaataac tcacgcatat gtcgcgcacc accgccggaa gcggcctggt aggttgcaac
    64621 ggacacccaa tcaacaagat cattggcgaa taaaccaccc aacgacatca acatcaggct
    64681 tacggtacag ttaccgccaa caaaagtcct gatgccatta tttaatccgt cggtaatgac
    64741 gtcctgattg acggggtcaa gaataatgat ggcgtcatct ttcatgcgca gagacgatgc
    64801 tgcgtcaatc cagtaaccct gccatccgct ttcacgaagc tttggataga tttcgttggt
    64861 ataatcgccg ccctgacagg tcacaatgat atcgagggcc tttagcgcct ccagatcaaa
    64921 ggcatcctga agtgtgccag tggttccgcc aaaagacggc gcagcctgac caagctgaga
    64981 agtagaaaag aagacagggc gaatggcgtc gaagtcgcgc tcttcaacca tgcgttgcat
    65041 gagaacggag ccgaccatac cgcgccagcc gataaaacca acatttttca taagcgtttt
    65101 ttcctgcaaa gatgtgtgct gtataaatgt gccggtctcc tcttggcaca tctttcacca
    65161 tacaaaaagc agccaaagtc gcaagtgaaa ttaatcaatg atagcgaagc catcagtaat
    65221 gcgacttatc ctgctttgtt agcacgcaga aagtccgcgg caattatcag ggaatttgag
    65281 ttatgaatga aatcatttct gcagcagttt tattgatcct gattatggat ccgctcggaa
    65341 acctacctat tttcatgtcc gtactgaaac atactgaacc gaaaagacgg cgggcaatca
    65401 tggtgcgaga gttgcttatt gctctcctgg tgatgctggt gttcctgttt gcgggtgaga
    65461 aaattctggc atttctcagc ctgagagcag aaaccgtctc catttctggc ggcatcattc
    65521 tgtttctaat tgccattaag atgattttcc ccagcgcttc aggaaatagc agcgggcttc
    65581 cggcaggtga agagccattt atcgtgccgc tggcaattcc gctggtcgcc gggccaacta
    65641 ttctcgccac gctgatgttg ttgtctcatc agtacccgaa tcagatggga catctggtga
    65701 ttgctctgct gctggcctgg ggcggcacct ttgtcatcct gctacagtct tcgctatttt
    65761 tacgtctgct gggcgagaaa ggggtgaacg cacttgaacg cctgatggga ttgattctgg
    65821 tgatgatggc aacccagatg ttcctcgacg gcattcgaat gtggatgaag gggtaaggtt
    65881 caaacaggtg ctgcacacaa ccagacgcgg cacctgtttt attacctgaa ctgataatga
    65941 ccttcgccca atcggataac ccttccctct tcttctgcgc ggttcagcca cacactaatt
    66001 tgttcgatgg ttaaaccagt acaagacgcc agcctttcaa tacttatcga ttcctttgca
    66061 aggatcgcca gttccgccac aaagagttga taaaaatcga caggcgtata cgcgggcatt
    66121 actgcgtaat ttgcgtcatc ttcatgcagc gaaaacagtt ctggttgagc gttacggagt
    66181 gctctgtcat acatcagccc aggaacgtct ggcagtctga tcgtcacatt ctcagcctgt
    66241 tcagcactcc atgacgcccc attttctact aatcgtgaat tggctgattg catatcctga
    66301 tttggtttaa cctgcacagg cagccactga tgttttaatg cctccatcgc cccggttatc
    66361 gttccaccgg tcattcccgc acgtacaacc attgcgcttt cagcaaggca ataaatatat
    66421 ttatttcgcg ccatcgcatt gccgacggtg aaacgtacct ctgggtaaaa cggcgaaatc
    66481 aacaccaggt tgcctgctat aagcccttca cgccatttca ctaacgtact cgtttttaac
    66541 aagctatcag ctaatacgcc aacagcagtt cccccggcct ccagtgccga cgccattgca
    66601 cattcatcaa tacctcgcgc accaccagag ataacgcaaa tcccctgttg ggccagtttc
    66661 gcggccagtt gttgggtata gcgcaaatcg tcagtcggag catctcgcga gccaacaatc
    66721 gccatacctt ccgcttgcag taatgcttta ttcccgcagc caaataaaac gggaggtgca
    66781 tccgttcgca atcggttttt caagcgaacg ggataatcag catctccgcg ggttaagatc
    66841 cataaaccgg cgcgttgcca cttatctacc gccaacgcca gactatgacc acgcgccagc
    66901 aagccaagaa tacgctcctg agaaatacgc ggatcgctcc agcctgtaag tttctcttgc
    66961 ggttgcggca ccagaagctc ggcgggactg atacgttggt gcttcagcca taatgcaaac
    67021 ttcccccatt cactattact aagaggttta tactcactcg ccgccgcgcg agaaaaatcg
    67081 ctggttagca ggagagttgc ttgtgcattg gctgaaagat tcatcaattt tttaccgagg
    67141 tagaagcaag ggcaagagga taaaccgtcg ggcaacctgc ctggcgtaac agtgctgtca
    67201 gaactgtcag cgtccatgca gaatccacga tatcgtcaac cagcaacgcc ggaccaggca
    67261 tcaaaggagg ggtaatcaca aacgccccgt cgagattttg acactggtgg aaacggtttt
    67321 gctgcatttt ctgcggtgga ttgtccacga ctttttcaat ggcatcaata aaaggtaagc
    67381 caagtttcgc cgccagtcgc cgggcaaaat caggaaccag gtcgaggtgc tttaatgaag
    67441 gaacgcagca tacccaggtt ggttcaggat gcggattcca gcgttggcga accatttccg
    67501 cacatgcctc caccagttca tcactgaatc gccccgtttt tttctcctgt gctacctgct
    67561 ttccccaccc ggaatctccc caacgagaaa ggattcgccc cgtagatcct tgtaaactcg
    67621 caggcaagtt ccctttaaat ccgtattgcg taaaagcccc agctgccacc tgtttattga
    67681 gatttaatgg caggtcagcg tgtttaataa ataagtttgc cgcatggagt aagccgctat
    67741 caatgtcggg cgacaacaac ggatgctgaa gacaactgct acatttacca caatgttcgg
    67801 cacctggctc atcgagtgcg cggcgcagaa attgcatttt gcactccttg gtcgtgatat
    67861 agctttcaac gtctgccagc tcactttttc tctgattcat caaatggtta attcgttcat
    67921 ggtcaggaga aaaactgaca attgttctgc gccataattt ctcggtatac accacaggcg
    67981 atggattttc agctaccaat aatttcagtg ctttttctat ttgcccataa cggagattcg
    68041 tccgttgttc aatgcctcgt aaggtaagac cgtcattcac gctaagtacg ttgagtattt
    68101 catgaatttg cgcctccgca ggaaaggcgc tttcacggaa gaatttatga atagcacggt
    68161 cttcactgcc gcaaagcagt atgccaacag cagaatcgat accgcgaccg gcacgcccca
    68221 cctgttggta gtaaccaact atcgaacctg gcatctggta atgaataaca aaccccaaat
    68281 ccggtttgtc aaagcccatt cccagcgccg ttgtcgcaac cagcacttta attttatttg
    68341 ccagcaacgc ctgctccaga tattccctgg cagtattaga atcttccgcg ccttcgcaag
    68401 tcacgccgct gtagtaagca aatgcactga taccattttt ccttaaccac tcggcgacaa
    68461 gttcggcatc gcgagtagtc agggtataaa caattcccga tttggaaaac tgagggataa
    68521 ccgttgctaa ccatgccaga cgggatgact gttcacctaa gactaaggca tcaagcgcca
    68581 aactttcgcg agccagcgtt ccacgctgaa tcacaatgtc acccaattgc tgacggatat
    68641 cctcaacgac acggttattc gctgtcgcgg ttgtacccag aacaggggta ttcgcaggta
    68701 gttggcgcaa aatatctaat atacgtcggt aatccgggcg aaaatcgtgg ccccaatcag
    68761 agatgcagtg cgcctcgtcg accacaagca agccaatacg atcggctata ggatataaaa
    68821 ccgtttcgat aaagtcctgg tttgccaaac gttccgggga aaccagcaag caatcgacct
    68881 caccttgcag caacttatcg ctaatgcgct gccattcttc tctgtttgta gaattcaacg
    68941 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
    69001 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
    69061 agtacacagc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgttggt
    69121 tcaccaccgc gtctatggct tcccattgcc cttccctgaa agtagcggtg gtatttccta
    69181 acatccgctg taaaagcaat tcagctgcgt gcttttccat ctttatctcc tgcgatcttt
    69241 aggcagtgat tagaacggat tatttgcagg taaatttgcg agagagatca aaaagatgaa
    69301 gagaggagaa aagagaaatg aattactgcg agcaagctcg cagtaatttg tcgcagtaat
    69361 taactcaaca gctggaacgc aatcatccca acgatggcac cgacagtgcc gaggatggtt
    69421 tccatcatgg tccaggtttt cagcgtttcg gcttcggtcg cgccggtaaa tttaccgaac
    69481 aaccagaaac cagcgtcgtt aacgtggctg acaacaatcg aaccaccggc gatacaaatc
    69541 gacagcgccg ccatttgcgc accggagtag ttcagttgtt caataaccgg catcaccagt
    69601 cccaccgccg ttaaacaggc aacggttgca gaaccctgaa tgatgcgcac tgcagcggcc
    69661 agcacgaagc aggtgatggc aatcggcagg cccatgccgg ttaacgcttc acccagcgcc
    69721 ggacctacgc cagagtcaac cagcacctgt ttgaacacgc cgcctgcacc aatcaccagc
    69781 agaatgatcc ccgccggttg cagcgcgtga ccgcaaatct ccatcacttt gtcttttggc
    69841 atgccctgac gcattgccag gccataaatc gctaccagac aagcaaccag aatcgcggta
    69901 aacggatgac caataaactc gaaccattcg taagcggtag agccttccgg cacaaaacgc
    69961 gcggcaatgg ttttcagccc caccagcacc agcggcagca ggatcagcga caggctgaat
    70021 ccgaaggacg gcattttgcc ttcgccgaga tgcggctcgc tgatgtcgtc aggaatatgc
    70081 agctcaacgt aacggctgat gaagttaccc cacagcggcc cggcaataat cattcccgga
    70141 attgccgcac acaggccaat caggatcatc cagccaaaat cggcgttcat ctgcgatgcc
    70201 agcagcattg gcgctggccc tggcaccagg aacgccgccg ctgccgccac gcctgcgaat
    70261 aatgggatta ccagcttcac caggttcgta ccggtgtggc gcgccattga gaaagcaacg
    70321 ctaatcagca gaacaatcgc cacttcaaag aacagcggta gcgcacagac cagccccgca
    70381 aggccgatgg cataatgcgc gcggctgtga ccgaaggatt taagcatttt gacggcaatc
    70441 tgatcgactg cgccggtttc atgtaagatc ttgccaaaca tagctcccag ggcgacaacc
    70501 accgccagga agccgagggt gcctcccatc cctttttcca tcgtcgctgc gattttatcg
    70561 agcggcatac cggaaaaaag gccagccccc atggacacca ccattaaagc caggaaagcg
    70621 tgcatacgcg ccttcatgac taaaaacagc agcagtaaaa cagaccctac tgctgttaaa
    70681 acaagcgtta atgtagtcac tacttatttg ccttttttaa taacctcaat ggtgcttgcc
    70741 acaacacctt ccagcggttg atcgatatcc accaccagta catcggtttc gtccgcaccc
    70801 ggctcctgca gcgtttcaaa ctgcgtcacc aacatttggg ttttaaagaa atggcctttg
    70861 cgcgctttca ggcggctttc aatcacatca aaatcgcctt tcaggtagat gaaagagaga
    70921 ttcggattac cttcacgcag caagtcgcga tagtgttttt tcaatgcaga acagacgatc
    70981 agcgacactt tattagtgcg ctgcatagca aacgcggcgt cgttcagcgc ctgcaaccac
    71041 ggtttgcgat cgtcgtcatt cagcggttcg ccagacgcca ttttttcgat attgcgccgt
    71101 ggatggagga aatcgccatc aagaaacgcg gcatgaagtt gatgcgccac ttcacttgcg
    71161 accgcagatt tgccgctgcc cgatacgccc atcaagacgt aaatgtggtg atcatggtta
    71221 gtcgtgctca aagtggtgcc cccacaatac aagaattaaa aatgttacgg gtaactgtta
    71281 tcggtaacat tctccggccg gacaatatca gaagcaatat ccatccgtgc cttaagtgta
    71341 taagtgtgag ctacttcaaa tttgtgggct taaatagatc cgcccggtga caaggtgaaa
    71401 cctaaatcta acattttcgg tgtcacagat tcgccacgaa tacgcgccag aaggcgttca
    71461 gcgccaatac tgcccatccg ctcacgcggc gtcagcacgc tcgcaagtcg tggctccatc
    71521 acctgaccaa tgtcatgacc gtggaaaccg gcaatcgcca tatcgtcagg aacttttaac
    71581 cccagacgct gacattcaaa cgccgcgccg accgccagat catcattagt acagaacacg
    71641 ccatccagct gcggatattc ccgccgcgcc tggcgaatca gttcaatacc ggaagagtaa
    71701 gaagaagatt gctcaaccat cacgctatac ggcaccaggc ctgcatcccg catcgcctgt
    71761 tcgtatccct tctgtttgat gatagtacgt tcgtcgagac gtgcgccgag ataggcaata
    71821 tggcgatgcc cgcgagcaat aatggcggcg gtcatctggc gtgccgcttc aaagttatca
    71881 aaaccgacgg caatatcgag gcatggcgac ttgctgtcca tcagttccac cacgggaatc
    71941 cccgccactt caatcatctt taaggtgcgc ggcgtgtggg tacgttcggt gaggataagt
    72001 ccgtcgatat tccatgagag cattgattcg agacgttctt gctccatttc cggtttataa
    72061 ccgtagtggg ccagcatggt ctgataaccg tgcgcgtcgg tgacgctttc gattccgcgt
    72121 aatacttccg cgaaaacctg gttggtgaga gaaggtaaca ggacgccaat cgcccggctg
    72181 gtggcgttag agaggatatc gggcgcacga ttgggaatat agcccagttc atcaagagcc
    72241 gcggcaatct tgccgcgtag agcgacggaa acctgctccg ggttgcgtaa aaaacggctg
    72301 accgtcattt tggtcacgcc tacacggtca gccacatcct gaagtacggg tcttttcttt
    72361 ttcatcgtcc tgaaggtaca aaagagatag attctcttag tttaacacgg acgcatcaga
    72421 accttcccgg ggaaaacagt gcttgtttac aattattaga tatacactaa atcaattgca
    72481 tcgcattgtg ctaacgacgc ggcaaaaccc cggaagcgta catcagcacg cgaccggagc
    72541 gagcgaggaa gcctgcaaaa gggcaggctt caaagatgcc gttgagtttt aaaccggcgg
    72601 cagatcgaac agtaacactt cgctgtcgct atccgcatgg atggagattg cctgctcatc
    72661 ccagattgcc agaccatcgc tggtcgaggc tttcacgccg ttaatggtga cattgccttt
    72721 caccacctgg atccagacgc ggcgttcagc ggcaatctga tgcaccgact gctcatcttt
    72781 cagcaacgcc cagcggtaca gttccatatc ctgatgcact ttcaatgaac catctcgcgc
    72841 atctggcgaa agcaccagtt gtttgccctg cacggcatcg aagcgacgct gttcataacg
    72901 cggcgtaata ccgttttctt caggcatgat ccagatctga tacagatgca gacgctcggt
    72961 gctgcttggg ttgtactctg agtgacgaat acccgtaccc gcactcataa tctggaactc
    73021 gcccgccgga acctgctctt tattgcccat gctgtcctgg tgctcaacag taccttccag
    73081 cacgtaggtc aaaatttcca tatctttatg cgggtgagtg ccgaagccct gccctgcttc
    73141 aatcacgtcg tcgttaatca cgcgcagcgc ggagaagccc ataaagttcg gatcgtaata
    73201 gttggcaaaa gagaaagtat gccaggagtc cagccagcca tgatttgcat gaccacgttc
    73261 atttgctttg cgtaagtaga tcatttgtat tcgccccctg aatgatttcg atgggcttag
    73321 tgtggacccg atctgcctgg gatgatagag ggtgaaaatt gacccctctg ttcaaaaaaa
    73381 ttgaactatt tgaggggcca tcaaacttac ttcgcgagag tcacggtgga gggagaggct
    73441 tgctcgaatc cgcgttcaag gatttccaga ttcgtaagaa cttcagattc cttgacgtaa
    73501 tttggcgcac cgtgggtgat ggtttgatac aacgcatcat aaacgcgccc gtaatcgccc
    73561 atctccggct tcatctcttc tctgaccgtc acgccctcgt cattgacata ctccagcaca
    73621 ccgaccgaat catccgctgc gaatcccggt tcgcccggca taatattagc cttcaggctg
    73681 gtttcctgct ggtcgatacc gtatttaata aacgaacctt tcttaccgtg aacgataaat
    73741 ttcggataat cgattttcac cagatggctg gttttgacga tggcttttag atcgccataa
    73801 aacagttgcg cttcgaaagt atcgtcagga ttggctttat tgcgcaggct gcggatgtca
    73861 taagcgacgt gatccgggcg accgaacaga gaaataatct ggtccatcgt atgcacacca
    73921 aggccataga acgcgccatc ctgcggcagc ccaggtttgg tttctgccac cgggcggtaa
    73981 tagtcaaaat ggctttccac ttcgacaatc tctcccagct tgccgctttc aatcgctttt
    74041 ttcgctgtca ggaagcagga atcaaagcga cgattctgat atggcgtgac ggtcagccct
    74101 ttgcttttcg ccagtgcaaa cagctctttc gcctgcgcaa gtgtcggagt gaacggtttt
    74161 tcgaccagca catttttccc ggcttccagc gcgcgtttcg cgtactcgaa atggctgtcc
    74221 gcgtgggtgc agaccacaac cagcttaaca tcgggatcgt ttagcacttc gtcgagatcg
    74281 ctggtgaaat ggatatgaga gtaaatggga gcctgttctt ccggcttcgc atggcgacga
    74341 aaaatatgcg cgacatgcca gctatccttg cggttaagta catacggcag atggtaacgg
    74401 gtggtgcttt tgccgaagcc aataaaggcg cagttgatga ccatgatttc gtccttttta
    74461 aggtggttat ttacacctta gcgcaaagcg gacgtggttc ctactggagt gcgcataacg
    74521 aacacaagca cttcccgagg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
    74581 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
    74641 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
    74701 tgcaaaaaaa atgcatttga ctcgcattcg aaagtcaatg atgttgaaag ggacatttac
    74761 cccaaagagg acaaaggaat gagtgagata gtgatacgcc atgctgaaac gcgcgattac
    74821 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacatt acaggtgcct
    74881 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caaacagtta
    74941 gttgcctgta ttgatggaac cgtcgtgggt cacctcacca ttgacgtgca acagcgccca
    75001 cgccgcagtc atgttgccga ttttggtatt tgtgtcgact cccgttggaa gaatcgcggc
    75061 gtcgccagcg cccttatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
    75121 cgtatcgagc taacggtgtt cgtcgataac gcgccagcaa ttaaggtcta taaaaaatac
    75181 ggctttgaaa ttgaagggac tggtaagaag tacgcattgc gtaatggtga atatgtcgat
    75241 gcgtattata tggcgcgggt gaagtaaaga atattctcaa attgcctgtt accaatgtga
    75301 ataattagtt ttgttcatct aatttgagtt tttccttata ttacca
//
LOCUS       DAZGBC010000006        69388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGBC010000006 DAZGBC010000000
VERSION     DAZGBC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.006"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            90..524
                     /gene="slyA"
                     /locus_tag="ACHK1J_000389"
     CDS             90..524
                     /gene="slyA"
                     /locus_tag="ACHK1J_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO7909728.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(571..1038)
                     /gene="slyB"
                     /locus_tag="ACHK1J_000390"
     CDS             complement(571..1038)
                     /gene="slyB"
                     /locus_tag="ACHK1J_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO7909729.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1312..2421
                     /gene="anmK"
                     /locus_tag="ACHK1J_000391"
     CDS             1312..2421
                     /gene="anmK"
                     /locus_tag="ACHK1J_000391"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO7909730.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2519..2848
                     /gene="mliC"
                     /locus_tag="ACHK1J_000392"
     CDS             2519..2848
                     /gene="mliC"
                     /locus_tag="ACHK1J_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO7909731.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNSRR"
     gene            2907..3563
                     /gene="pdxH"
                     /locus_tag="ACHK1J_000393"
     CDS             2907..3563
                     /gene="pdxH"
                     /locus_tag="ACHK1J_000393"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO7909732.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3692..4966
                     /gene="tyrS"
                     /locus_tag="ACHK1J_000394"
     CDS             3692..4966
                     /gene="tyrS"
                     /locus_tag="ACHK1J_000394"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO7909733.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5025..5888
                     /gene="pdxY"
                     /locus_tag="ACHK1J_000395"
     CDS             5025..5888
                     /gene="pdxY"
                     /locus_tag="ACHK1J_000395"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO7909734.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5932..6537)
                     /gene="gstA"
                     /locus_tag="ACHK1J_000396"
     CDS             complement(5932..6537)
                     /gene="gstA"
                     /locus_tag="ACHK1J_000396"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO7909735.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6643..8145)
                     /gene="dtpA"
                     /locus_tag="ACHK1J_000397"
     CDS             complement(6643..8145)
                     /gene="dtpA"
                     /locus_tag="ACHK1J_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO7909736.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(8755..9390)
                     /gene="nth"
                     /locus_tag="ACHK1J_000398"
     CDS             complement(8755..9390)
                     /gene="nth"
                     /locus_tag="ACHK1J_000398"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO7909737.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(9390..10085)
                     /gene="rsxE"
                     /locus_tag="ACHK1J_000399"
     CDS             complement(9390..10085)
                     /gene="rsxE"
                     /locus_tag="ACHK1J_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO7909738.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADSLLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(10089..10709)
                     /gene="rsxG"
                     /locus_tag="ACHK1J_000400"
     CDS             complement(10089..10709)
                     /gene="rsxG"
                     /locus_tag="ACHK1J_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO7909739.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(10713..11771)
                     /gene="rsxD"
                     /locus_tag="ACHK1J_000401"
     CDS             complement(10713..11771)
                     /gene="rsxD"
                     /locus_tag="ACHK1J_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO7909740.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLTLCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(11772..14090)
                     /gene="rsxC"
                     /locus_tag="ACHK1J_000402"
     CDS             complement(11772..14090)
                     /gene="rsxC"
                     /locus_tag="ACHK1J_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO7909741.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAISRA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQPANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVP
                     EEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(14083..14661)
                     /gene="rsxB"
                     /locus_tag="ACHK1J_000403"
     CDS             complement(14083..14661)
                     /gene="rsxB"
                     /locus_tag="ACHK1J_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO7909742.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(14661..15242)
                     /gene="rsxA"
                     /locus_tag="ACHK1J_000404"
     CDS             complement(14661..15242)
                     /gene="rsxA"
                     /locus_tag="ACHK1J_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO7909743.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(15319..15759)
                     /gene="ydgK"
                     /locus_tag="ACHK1J_000405"
     CDS             complement(15319..15759)
                     /gene="ydgK"
                     /locus_tag="ACHK1J_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO7909744.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(15845..16060)
                     /gene="ydgT"
                     /locus_tag="ACHK1J_000406"
     CDS             complement(15845..16060)
                     /gene="ydgT"
                     /locus_tag="ACHK1J_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO7909745.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(16333..16458)
                     /gene="blr"
                     /locus_tag="ACHK1J_000407"
     CDS             complement(16333..16458)
                     /gene="blr"
                     /locus_tag="ACHK1J_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO7909746.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            16701..17741
                     /gene="ydgJ"
                     /locus_tag="ACHK1J_000408"
     CDS             16701..17741
                     /gene="ydgJ"
                     /locus_tag="ACHK1J_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7909747.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKSGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(17776..18777)
                     /gene="add"
                     /locus_tag="ACHK1J_000409"
     CDS             complement(17776..18777)
                     /gene="add"
                     /locus_tag="ACHK1J_000409"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO7909748.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLDHGIRASIN
                     TDDPGVQGVDIIHEYNVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(18881..20053)
                     /gene="malY"
                     /locus_tag="ACHK1J_000410"
     CDS             complement(18881..20053)
                     /gene="malY"
                     /locus_tag="ACHK1J_000410"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO7909749.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNATFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(20063..21655)
                     /gene="malX"
                     /locus_tag="ACHK1J_000411"
     CDS             complement(20063..21655)
                     /gene="malX"
                     /locus_tag="ACHK1J_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO7909750.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGIVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            21830..22858
                     /gene="malI"
                     /locus_tag="ACHK1J_000412"
     CDS             21830..22858
                     /gene="malI"
                     /locus_tag="ACHK1J_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO7909751.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            22970..23737
                     /gene="hdhA"
                     /locus_tag="ACHK1J_000413"
     CDS             22970..23737
                     /gene="hdhA"
                     /locus_tag="ACHK1J_000413"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO7909752.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            23966..24556
                     /gene="uidR"
                     /locus_tag="ACHK1J_000414"
     CDS             23966..24556
                     /gene="uidR"
                     /locus_tag="ACHK1J_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO7909753.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            24947..26758
                     /gene="uidA"
                     /locus_tag="ACHK1J_000415"
     CDS             24947..26758
                     /gene="uidA"
                     /locus_tag="ACHK1J_000415"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO7909754.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNYASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            26755..28128
                     /gene="uidB"
                     /locus_tag="ACHK1J_000416"
     CDS             26755..28128
                     /gene="uidB"
                     /locus_tag="ACHK1J_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO7909755.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            28167..29432
                     /gene="uidC"
                     /locus_tag="ACHK1J_000417"
     CDS             28167..29432
                     /gene="uidC"
                     /locus_tag="ACHK1J_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO7909756.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(29477..30985)
                     /gene="ydgA"
                     /locus_tag="ACHK1J_000418"
     CDS             complement(29477..30985)
                     /gene="ydgA"
                     /locus_tag="ACHK1J_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7909757.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(31086..32261)
                     /gene="manA"
                     /locus_tag="ACHK1J_000419"
     CDS             complement(31086..32261)
                     /gene="manA"
                     /locus_tag="ACHK1J_000419"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO7909758.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIL
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            32460..34106
                     /gene="fumB"
                     /locus_tag="ACHK1J_000420"
     CDS             32460..34106
                     /gene="fumB"
                     /locus_tag="ACHK1J_000420"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7909759.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            34249..35652
                     /gene="fumC"
                     /locus_tag="ACHK1J_000421"
     CDS             34249..35652
                     /gene="fumC"
                     /locus_tag="ACHK1J_000421"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO7909760.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(35649..36578)
                     /gene="tus"
                     /locus_tag="ACHK1J_000422"
     CDS             complement(35649..36578)
                     /gene="tus"
                     /locus_tag="ACHK1J_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO7909761.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHNPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(36654..37955)
                     /gene="rstB"
                     /locus_tag="ACHK1J_000423"
     CDS             complement(36654..37955)
                     /gene="rstB"
                     /locus_tag="ACHK1J_000423"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO7909762.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(37959..38678)
                     /gene="rstA"
                     /locus_tag="ACHK1J_000424"
     CDS             complement(37959..38678)
                     /gene="rstA"
                     /locus_tag="ACHK1J_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO7909763.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            38807..39142
                     /gene="ydgC"
                     /locus_tag="ACHK1J_000425"
     CDS             38807..39142
                     /gene="ydgC"
                     /locus_tag="ACHK1J_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO7909764.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(39139..39861)
                     /gene="folM"
                     /locus_tag="ACHK1J_000426"
     CDS             complement(39139..39861)
                     /gene="folM"
                     /locus_tag="ACHK1J_000426"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO7909765.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(39898..41280)
                     /gene="ydgI"
                     /locus_tag="ACHK1J_000427"
     CDS             complement(39898..41280)
                     /gene="ydgI"
                     /locus_tag="ACHK1J_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7909766.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(41466..42410)
                     /gene="ydgH"
                     /locus_tag="ACHK1J_000428"
     CDS             complement(41466..42410)
                     /gene="ydgH"
                     /locus_tag="ACHK1J_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO7909767.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            42934..44466
                     /gene="pntA"
                     /locus_tag="ACHK1J_000429"
     CDS             42934..44466
                     /gene="pntA"
                     /locus_tag="ACHK1J_000429"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO7909768.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            44477..45865
                     /gene="pntB"
                     /locus_tag="ACHK1J_000430"
     CDS             44477..45865
                     /gene="pntB"
                     /locus_tag="ACHK1J_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO7909769.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(45890..46924)
                     /gene="tqsA"
                     /locus_tag="ACHK1J_000431"
     CDS             complement(45890..46924)
                     /gene="tqsA"
                     /locus_tag="ACHK1J_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO7909770.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            47336..47701
                     /gene="mdtJ"
                     /locus_tag="ACHK1J_000432"
     CDS             47336..47701
                     /gene="mdtJ"
                     /locus_tag="ACHK1J_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO7909771.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            47688..48017
                     /gene="mdtI"
                     /locus_tag="ACHK1J_000433"
     CDS             47688..48017
                     /gene="mdtI"
                     /locus_tag="ACHK1J_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO7909772.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(48056..48877)
                     /gene="ydgD"
                     /locus_tag="ACHK1J_000434"
     CDS             complement(48056..48877)
                     /gene="ydgD"
                     /locus_tag="ACHK1J_000434"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO7909773.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(48977..49060)
                     /locus_tag="ACHK1J_000435"
     CDS             complement(48977..49060)
                     /locus_tag="ACHK1J_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7909774.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(49153..49461)
                     /gene="asr"
                     /locus_tag="ACHK1J_000436"
     CDS             complement(49153..49461)
                     /gene="asr"
                     /locus_tag="ACHK1J_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO7909775.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            49732..49836
                     /locus_tag="ACHK1J_000437"
     CDS             49732..49836
                     /locus_tag="ACHK1J_000437"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO7909776.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(49885..51138)
                     /gene="ynfM"
                     /locus_tag="ACHK1J_000438"
     CDS             complement(49885..51138)
                     /gene="ynfM"
                     /locus_tag="ACHK1J_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7909777.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            51245..52138
                     /gene="ynfL"
                     /locus_tag="ACHK1J_000439"
     CDS             51245..52138
                     /gene="ynfL"
                     /locus_tag="ACHK1J_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7909778.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            52273..53493
                     /gene="mlc"
                     /locus_tag="ACHK1J_000440"
                     /gene_synonym="dgsA"
     CDS             52273..53493
                     /gene="mlc"
                     /locus_tag="ACHK1J_000440"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO7909779.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            53618..54313
                     /gene="bioD"
                     /locus_tag="ACHK1J_000441"
     CDS             53618..54313
                     /gene="bioD"
                     /locus_tag="ACHK1J_000441"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7909780.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(54266..55558)
                     /gene="clcB"
                     /locus_tag="ACHK1J_000442"
     CDS             complement(54266..55558)
                     /gene="clcB"
                     /locus_tag="ACHK1J_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO7909781.1"
                     /translation="MHHLHIYPNLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(55719..56333)
                     /gene="dmsD"
                     /locus_tag="ACHK1J_000443"
     CDS             complement(55719..56333)
                     /gene="dmsD"
                     /locus_tag="ACHK1J_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO7909782.1"
                     /translation="MTHFSQQDNFSVVARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGKLARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(56376..57230)
                     /gene="ynfH"
                     /locus_tag="ACHK1J_000444"
     CDS             complement(56376..57230)
                     /gene="ynfH"
                     /locus_tag="ACHK1J_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO7909783.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(57232..57849)
                     /gene="ynfG"
                     /locus_tag="ACHK1J_000445"
     CDS             complement(57232..57849)
                     /gene="ynfG"
                     /locus_tag="ACHK1J_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO7909784.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(57860..60283)
                     /gene="ynfF"
                     /locus_tag="ACHK1J_000446"
     CDS             complement(57860..60283)
                     /gene="ynfF"
                     /locus_tag="ACHK1J_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO7909785.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPKMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(60344..62770)
                     /gene="ynfE"
                     /locus_tag="ACHK1J_000447"
     CDS             complement(60344..62770)
                     /gene="ynfE"
                     /locus_tag="ACHK1J_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO7909786.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(62968..63273)
                     /gene="ynfD"
                     /locus_tag="ACHK1J_000448"
     CDS             complement(62968..63273)
                     /gene="ynfD"
                     /locus_tag="ACHK1J_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO7909787.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            63381..64091
                     /gene="ynfC"
                     /locus_tag="ACHK1J_000449"
     CDS             63381..64091
                     /gene="ynfC"
                     /locus_tag="ACHK1J_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO7909788.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(64094..64654)
                     /gene="speG"
                     /locus_tag="ACHK1J_000450"
     CDS             complement(64094..64654)
                     /gene="speG"
                     /locus_tag="ACHK1J_000450"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO7909789.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(64689..65030)
                     /gene="ynfB"
                     /locus_tag="ACHK1J_000451"
     CDS             complement(64689..65030)
                     /gene="ynfB"
                     /locus_tag="ACHK1J_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO7909790.1"
                     /translation="MKITLSKRIGLLAFLLPCTLALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            65165..65491
                     /gene="ynfA"
                     /locus_tag="ACHK1J_000452"
     CDS             65165..65491
                     /gene="ynfA"
                     /locus_tag="ACHK1J_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO7909791.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            65697..66911
                     /gene="rspA"
                     /locus_tag="ACHK1J_000453"
     CDS             65697..66911
                     /gene="rspA"
                     /locus_tag="ACHK1J_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO7909792.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTITPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            66923..67942
                     /gene="rspB"
                     /locus_tag="ACHK1J_000454"
     CDS             66923..67942
                     /gene="rspB"
                     /locus_tag="ACHK1J_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO7909793.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHVDGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWVINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            68000..69367
                     /gene="ydfJ"
                     /locus_tag="ACHK1J_000455"
     CDS             68000..69367
                     /gene="ydfJ"
                     /locus_tag="ACHK1J_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO7909794.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
BASE COUNT        17459 a        16983 c        17846 g        17100 t
ORIGIN      
        1 agcaatacat ttgttttagc aatacaattg ctgcacacta ttctaaaagc cgcataatat
       61 cttagcaagc taattataag gagatgaaat tggaatcgcc actaggttct gatctggcac
      121 ggttggtgcg catatggcgt gctctgatag accatcgcct gaaaccgctg gagttaacac
      181 aaacccattg ggttacgtta cacaatatcc atcagttacc tccagaccag tcgcaaattc
      241 aactggcaaa agcgattggc atcgagcagc catcactggt ccgtactctg gaccaactgg
      301 aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct aaacgtatta
      361 aactgacgga aaaggcagag ccgctgatca gcgaaatgga agctgttatt aacaaaaccc
      421 gcgcggaaat cttacatggc atctccgcag aggaactgga acaactgatt acgctcatcg
      481 caaaacttga gcataatatc attgagttac aggccaaagg gtgaaatgaa gggggcgtgt
      541 ggtcagtaac ctgaccacac gcaaacttat ttagcgcgga gaaacggtca cctgactgcc
      601 attgctggcc agtacgacac gttggcccgg agagaaacga gtgttgcctt gtttctgtac
      661 caccatgatg gtattaccat cgtctttacg aatttccagc tcgacacctt gcgttttgtt
      721 cattgcactc tgtacgccct gaccagctac gccacctgca acagcgcctg ctgcagtagc
      781 cagagaacgc ccggttccgc caccaacagt atttcccagg aaaccaccaa gaacagcacc
      841 gccaattgca ccgataacgt tggaatcatc accgccctga atctgtaccg gacgtacgtt
      901 aacgatggtt ccatagctga cattctgtac ttgtttcgct tcagaagcgg tataaacatc
      961 ccctgacagg gtgtcgttat taacacaacc gacaagagac agacctacca ttgaaacaac
     1021 caatacgcgt ttaatcattg aaaaatctcc tgttcaccat gaaacgccac gcaagcatcc
     1081 ctcatggtca aagtatatgg catatgtgaa tccatggtta catgttctac caaatatgag
     1141 caaatcataa tgaaagtagg atcaaccaat tataaacaaa acattcatga tgattattga
     1201 tttgaagata atgttttact accaacggta aaaatcggtc attccgcatg gtattcacga
     1261 aaagtttatg tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac tatgaaatcg
     1321 ggccgcttta ttggcgttat gtcaggcacc agccttgatg gtgttgatgt tgtgttggcg
     1381 acaattgatg aacaccgggt cgcacagctg gcaagtttga gctggccgat cccggtgtct
     1441 ctgaaacagg ctgtactgga tatttgccag ggccagcagc ttacactttc gcagtttgga
     1501 cagcttgata ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt gcttaaggaa
     1561 caaaacctgc aggcgagaga tatagttgcg atcggctgtc acggtcaaac cgtctggcat
     1621 gaaccgacgg gcgtggcacc acacacttta cagattggcg ataacaatca aattgtggca
     1681 cgcaccggaa ttacggttgt cggtgatttt cgccgtcgcg atattgcctt gggaggacaa
     1741 ggcgcaccgc tggtacctgc gttccatcat gccttgctgg ctcacccaac cgagcgacga
     1801 atggtgctca atattggcgg catcgccaat ctgtcactgc tcattcctgg gcagccggtt
     1861 ggcggctacg ataccggtcc tggtaacatg ctgatggatg cctggatctg gcgtcaggcc
     1921 ggtaaacctt acgataaaga tgccgagtgg gcacgggcgg gtaaagttat tctcccactg
     1981 ctgcaaaata tgctcagcga cccatatttc tcgcaacctg caccgaaaag caccggacgc
     2041 gaatacttta actacggctg gctggagcgc catttacgcc attttccggg tgttgatccc
     2101 cgtgatgttc aggccacact ggcagaactc accgccgtga ccatttctga acaagttttg
     2161 ttgagcggtg gctgcgaacg attgatggta tgtggtggag gtagtcgtaa tccgctactc
     2221 atggcgcgtc tggcggcatt actgccaggc acagaagtca ccaccaccga tgccgttggc
     2281 attagtggcg atgacatgga agcattggct ttcgcctggc ttgcctggcg gacgctggcg
     2341 ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt actgggggct
     2401 attttcccgg ctaacccgtg atatcagagt taactgaatt tttcttaccg cgtctgccgt
     2461 tacacttgga acgttaggga gggcgtattg ccctccagac caggaaagtc ttcgggaaat
     2521 gtctatgaaa aaactgttac tgatctgttt gccggtgctt ctcaccggct gtagcgcgtt
     2581 caatcaactg gttgagcgca tgcaaaccga tacgctggaa taccagtgtg atgaaaaacc
     2641 gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg ataatcaact
     2701 actgcatctc aaacagggca tttcagcctc tggtgcacgt tacactgacg gaatctatgt
     2761 tttctggtcg aaaggcgagg aagcgactgt ctataaacgc gaccgcatcg tcttgaataa
     2821 ctgtcagtta caaaattcac ggcgttgaga tttttccagg ggcggcgcac aatagcgtca
     2881 cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag caaatcgcgc
     2941 atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc gccgatccat
     3001 taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg gaccctaccg
     3061 cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc gttttactca
     3121 aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt aaagcacatc
     3181 aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt gaacgccagg
     3241 tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa tattttcata
     3301 gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt cgcatttctg
     3361 cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa cagggcgaag
     3421 tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt gagttctggc
     3481 agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat gatgcgtgga
     3541 agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg ctggtactcc
     3601 tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa
     3661 aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga ttaaacaatt
     3721 gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag agcgactggc
     3781 gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct tgcatttggg
     3841 gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca agccggttgc
     3901 gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa
     3961 gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgcaagc aggttgcccc
     4021 gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact atgactggtt
     4081 cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct ccgttaacca
     4141 gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt
     4201 cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga acaaacagta
     4261 cggtgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt ctggtatcga
     4321 cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc tgatcaccaa
     4381 agcagatggc accaaatttg gtaaaactga aggcggcgca gtctggttgg atccgaagaa
     4441 aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg acgtttaccg
     4501 cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctgg aagaagaaga
     4561 taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg tgacccgtct
     4621 ggttcacggt gaagaaggtt tgcaggcggc aaaacgtatt accgaatgcc tgttcagcgg
     4681 ttccttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg gcgtaccgat
     4741 ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg aactgcaacc
     4801 ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta acggtgaaaa
     4861 acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc gttttacctt
     4921 attgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca ttaagtggaa
     4981 agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg aaaaatattc
     5041 tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca gagtttccga
     5101 tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct aatcacaccc
     5161 aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag
     5221 gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc tatctgggat
     5281 cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagcc gcgaatccgc
     5341 aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac
     5401 cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc attgcgccga
     5461 atcttgttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc
     5521 tggcagcgcg cgaactcatt gcgcaagggc cacaaattgt gttggttaaa cacctggcgc
     5581 gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat gaagcctggc
     5641 atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga
     5701 cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag gccctggaac
     5761 atgtgaccgc tgcagtatac gaaatcatgg tgaccaccaa agcaatgcag gaatatgagt
     5821 tgcaagtggt tgctgctcag gatcgtattg ccaaaccaga acattacttc agcgcaacaa
     5881 agctctgaaa tatctcattt cggcccacaa cgaactgtgg gccgacggct attactttaa
     5941 gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt gcataaatgc
     6001 cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc gcagtaccgt
     6061 aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct catccttcag
     6121 tgcctcgttc acatattgca gcttcttctc cagctgcgcg cgaactgtcg gtttgtactc
     6181 ttccggtgta tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg tggcgatgta
     6241 attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca gcaactggcg
     6301 gtcggggacg ctgtcggcaa gatactgcat aatcgctaca ccttccgtca gcaaagtacc
     6361 gtcatccagc agcaatgcag gtacctgtcc cttagggtta acggcaaagt aatcgtcacc
     6421 gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct ttccgctctc
     6481 acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca atttcatcgg
     6541 taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg
     6601 ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta cagcagcttt
     6661 cgccgctttg tcagcagcgt catcctgcgt catgcggtgc agtttcggcg cggtaaacag
     6721 catcagtact gcaataacgg cagtagcgac accaatctgc aagaatacgc gaccatagac
     6781 ttccagtgac atcagcggat cggtaacgtt atccggcaca gccatcatac ccgcaacata
     6841 accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat
     6901 cagacgctgc ggaacgagtt gagcaaccat tgccagaccc agaccagaga tcataagttc
     6961 cccgatactc tgcaggccat agcttgcgac cagccagctt acagacacga tacctgcgtc
     7021 agacgcgaat tttgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatacc
     7081 gattgcaaac ttagttggca tcggcagggt atcgcccatc ttgttataga tagcggccag
     7141 aatcgggcta ccaatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac
     7201 ggccaggccc agaatggtgt gttcaacgtt acgaatcgca aagaagttca gtgacgttgg
     7261 catctggctg tacagcacga agaagataat ggcttcgagc atcaggatga aggcaacgat
     7321 cattttacga cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga taaccacgat
     7381 accaaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca gcagccaggt
     7441 ggcgatagcg atcagtgcca caacaccaat aatggtcagc agcaggttac ggtagttgat
     7501 aggctcgaag tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt
     7561 aacaatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc agccgtattt
     7621 cgcggccagc cacggggtag cgatcataga gaagaaagag ccgatgttga cggacatgta
     7681 gtacatggtg aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag
     7741 agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata
     7801 aacgataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat
     7861 agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat
     7921 agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc
     7981 agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc
     8041 aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt gtttgaaagc
     8101 gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt
     8161 tacatcccat attaacgggg gtgctttctc gcgttatgcc taaaaacata cgcgcaattg
     8221 ttatagtggg aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct
     8281 ttgtaataat ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat
     8341 ccagcgttaa atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga
     8401 tgatggcttg acggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt
     8461 ttctgaaaaa tagccagtgt aatgttttgt aggtcaatat ttgttgcgtg tgtaaattta
     8521 aatgatttta acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc
     8581 acgaatgaat agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg
     8641 caaacggatt acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt
     8701 taaaagagga taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat
     8761 gtcaactttc tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt
     8821 gcgggcaatg caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa
     8881 ctctgccgga accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc
     8941 aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc
     9001 gaatgcagtg tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag
     9061 cgcagcacga tcttccggaa cctcgccatt atgctgctcc agtaagatac ggcaggtttt
     9121 gatgatattt tctgctttgc tgttataaag cccaatcgtt ttgatatagg ttttcacccc
     9181 ttcaacgccc agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt
     9241 cgccttatta acactgacat cggttgcctg agcggaaagc agtacggcaa tcagcaattc
     9301 aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt
     9361 gaggatctcc aggcgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat
     9421 gcacgttcgg cagctgcttc agtacggcgc tttttcattc tttcatcaat caggtatttt
     9481 cctgccagca tcagtcccag gccaataaat gcacctggtg gcagcatcgc cagcaggaaa
     9541 ggggagtcgg tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgaa
     9601 tctgcaccgt caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac
     9661 atggcgcagg ttgcgcccat accaattgaa aagccgtcca gtgccgaaag cgccggacct
     9721 tttttggcgg cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga
     9781 ataaaaatcc ctaatgattg atacaggcca aaagcgtagg cgttgatcag catctgtaca
     9841 gcgctgacca ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc
     9901 cagtgacgca gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt
     9961 cctaaaccca gagcgttagt ggcagtggac gtgaccgcca acagaggaca aaggccgagc
    10021 aactggacca gcgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg
    10081 ctcacgcttt attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg
    10141 tacaatccgg cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg
    10201 aactggtcga aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg
    10261 atttttttac ccgcaaaatg ggtgatccag tcagaaaggc gcagttcgat tttatcgcca
    10321 agccctggcg tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc gttaaaatcg
    10381 gctcccacca gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg
    10441 gcggctaccg gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac
    10501 tctggcgcag ttaccagata gcaactctgt gccagcgcat tgttatagcg ttcggctggc
    10561 agcacctgat caaataacgc cttttgttgc agactggcct gttcagcaat cgtcgttttg
    10621 gtcatctggt tgatggccgc agttaaccct gttgaacccg ctgcaaatag cgccaacgta
    10681 atgccgtgtt ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc
    10741 ggacgggtgt agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc
    10801 acgccgtcag gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgcaagcgcg
    10861 ccgaaaataa gacgaccacg attggtcgta gaagcggtaa ccgggtcagt caaaataaag
    10921 aatgcgccga gcatggtcgc tccagacagc agatgaattt gcggtgcggc cagtgtttct
    10981 ggtgagaaca accagcccaa cgttgcgcat aacgtcagcg ttactaagaa gctgagggga
    11041 atatgccagc gaatagcttt ctgccatagc aaccacacgc cgccagccag ccaggcgaga
    11101 tttacccatt gccaaccagc gcccgccagc ataccgctgt agatcggata ttgcataatc
    11161 tgttcaaccg aatgaccggc acggacagag gttttaaatg tatccagcgg tgtcgcctga
    11221 ctaatgccat caatacctaa gcgtagtgtg ttcatatcac caccactggc ggtatgcccg
    11281 ctgaaaataa cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc
    11341 ggtaaccagc tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt
    11401 gccggattaa acgggttttg ccccagaccg ccatacaact gtttagcgat gataaccgca
    11461 aacaccgtac ccagcacaac catccaccat ggcgcgaggg ggggaatact taccgccagc
    11521 aataagcctg tcagcaatgc tgagttatct ttcaacgtgg cggctaccga ctgcttgcgt
    11581 agtttgagta cgagagcttc ggctaacaga gcactaaccg atgccaacag gatctgaacg
    11641 agagtacccc aaccaaaaaa ccacagttgc gctgcgattc ctggcacggc tgcgagcaac
    11701 actaacagca taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt
    11761 ctgaatacca tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg
    11821 cgggcaatag ccgcggcaac tgccgctttg cgcggatcaa cctgttcttc tggtaccgca
    11881 ttagcctgtt gctgttccag cttgcgtgct ttggcgcgtg caatagccgc ttcgacggca
    11941 gctttgcgcg gatcaacctg ttcttctggc tccgcattag ccggttgctg ttccagcttg
    12001 cgtgctttgg cgcgggcaat agcggcttcg acggcggctt tgcgcggatc gacctgttct
    12061 tctggttccg cattagcctg ttgctgttcc agcttgcgtg ctttggcgcg ggcaatagcg
    12121 gcttcgacgg cagctttgcg cggatcgacc tgttcttctg gctccgcatt agcctgttgc
    12181 tgttccagtt tgcgcgcttt ggcacgggca atagcggctt cgacggcggc tttgcgcgga
    12241 tcgaccggtt cttctggttc cgcattagcc tgttgctgtt ccagcttgcg tgctttggca
    12301 cgggcaatag cggcttcgac ggcagctttg cgcggatcga cctgttgttc tggttccgca
    12361 tttgcctgtt gctgttccag cttgcgcgct ttggcgcggg agatagctgc ttcgacggca
    12421 gttttacgtg gatcagtaac ggttgctgcg tcgttagttt gctgcagttc tgcctgtttc
    12481 gctctggctt gcgctttacg ggcttcccgt gctgcaataa ttgcactgtt atccgggcgt
    12541 tcgcccgctt taatcacaat aggctgtgta gcctgggcct gtttctcttt cacccgcgcc
    12601 agagcggcag caatcgcatc tttatctttg gctgcaggtt gaacggctgc gctcttatgt
    12661 cgttcaaggc gagccgcttt ttcgcgctcc agacgagcct ggcgcgcttc gaaacgcgct
    12721 ttggcttctg cggcgcgctt ttcttcctga cgaatagccg caatttcagc tttttcctga
    12781 cggaaatatt gcaccagggg aatattgctc gggcaaaccc acgcgcaagc accacattca
    12841 atgcagtcgg caatgttatg cgtggtagct ttatcgtgtt gctgaccttt gctgaaccag
    12901 tacaactgtt gcggcaaaag atccgcaggg caggcgtcag cacaggcgct acaccggatg
    12961 cagctttgtt cttcctgtgg ttcgccaagc tcattggcag agggagccaa cagacagttg
    13021 gtaatcttta cgaccgggac atccagccat ggcaaggtaa agcccattag cgggccgccc
    13081 ataatcacca tttgatcggc agaggggcag aatccggcgt cattcaataa atgacgcact
    13141 ggcgtcccca gccgtgccca gacgttgccc gggcgagcga ttgcttcgcc agtcagggtt
    13201 acgacacgct cggtaatcgg ctcgccatca ataacggcac gtttcactgc ataagcagtg
    13261 ccgacgtttt gcattaatac gccgatatca gatgaacgcc cgccatgtgg aacctgcttc
    13321 ccggtcagaa tgtaggttaa ttgtttagca ccgccagaag gatatttggt tggaatcacc
    13381 cgcagagaaa tatcgtgaga gtccgccagc accgcgcgca gcatggaaat cgcctgcggt
    13441 ttgttatctt caatgccgat aagaatttcg cgtggctgca gaatatgcgc aagaatgcga
    13501 ataccctcta cgacctgagc cgcgcaatcc tgcatcaaac ggtcatcggc ggtaatgtac
    13561 ggctcgcact cagcagcgtt gataatcaac gtttcaatct tatctccgcc gccctgcaat
    13621 ttaacgcctg tcgggaatcc cgcaccaccc agcccggcaa caccaaactg atgaatgcgc
    13681 tcgattaact cttcgcgact gcgagagcga taatcggccc agccgtctcg tgggatccag
    13741 cagtcttcac catcggcatc aataatcacg cttaattcag ctaaagctga aggatgagcc
    13801 gtagagtggg gcgcaatagc cgtaacggta cccgaggtgg gcgcgtgaac aggcagcatt
    13861 ttgccgcgac cacgggtaag cggctggccg cgcaatactt tatcgccgac gctaacgcac
    13921 aactcacctt cagcgccaat atgctgtttc agtggaatga caaaacgctg cgccaggggt
    13981 acctggcgca ggggtgtacc gttggactgg gttttcatct ccggtggatg gatgccgccg
    14041 ttgaaatccc agattttatt ttttctgaat gcagagaata acttaagcat ggtgttccac
    14101 gggaatgata cgcacgggaa tggtgttcag atcccatttc caggagtcag gtgtttctgc
    14161 gaccggttgc aacgagatgc agtgcgtcgg gcacggatca acacataaat tgcagcccgt
    14221 acagagatca ctcattaccg tatgcatggc acgggtagcg ccaacgatgg cgtctaccgg
    14281 acacgcctga atacatttag tgcagccaat acagttattt tcatcaataa ccgccaccat
    14341 ccgcgcaggc gttagctctt gcgcttcgcc atccagcggc tgcggctcga cattaagcaa
    14401 ctcggcaatt tttagcatca cagcttcgcc acctggggcg caacggttga ttttttcacc
    14461 gttacagctg atggcttccg cgtaggggcg acagccggga taaccgcact gaccacactg
    14521 gctctgcggt aagatttcgt caattttctc aacgaccgga tcgtcttcca ccgcaaaacg
    14581 gcgggaggca taacccagaa tggcgccaaa cgccaggccc agcaggctca cggcggcaac
    14641 ggcaatccag atagcattca ttacaacttc accaaaccac taaagcccat aaaggccaga
    14701 gacataagac ctgcggtaat taacgcaatg gcattaccgc gaaaaggtgc cgggacatca
    14761 gccacagcaa ggcgttcgcg gatggctgcg aagagaacca tcaccagcga aaaaccgaca
    14821 gcggcggaaa aaccgtacag cgccgactgc aagaaattgt gcccgagatt gatattcagc
    14881 aacgccacgc cgagcacggc acagttggtg gtgataagcg gcaaaaaaat ccccagcaaa
    14941 cggtaaagca ccgggctggt tttgcgcacc accatctcgg tgaactgcac gaccacagca
    15001 atcaccagaa taaatgccag ggtgcgcagg taaatcagat taagtgggat caaaatccac
    15061 gtatcgataa gccaggcgca aatagacgcc agcgtcatca caaacgttgt tgccagcccc
    15121 atgcccatcg cggtttccag ctttttggaa actcccataa acggacagag accgagaaac
    15181 ttgaccagta caaagttatt gaccagtaca gttccaacaa acagtaacag gtagtcagtc
    15241 atttttcggc ctgaaataaa aaagccgcct attatccgtt aatccagagc aggcgacaac
    15301 aggttaactg taaggttatt acggattcac aaacgtcgct ttcacccgcg acgagcgttt
    15361 gaaatagggg acgatcaatg cggttgccaa caaagtaaac agcaactggc gaacggctat
    15421 gccatcttcc acgggtgaaa aagcgaacgc ttttactgcc agcaatacgg aaataagcag
    15481 ccagataata tagtgtttag gaacacaacg ccgacgttta aagaaagcga tggtcaacca
    15541 cagtgtgtaa taccacatgg cgatagcagt aataaaagaa acgccccata agatttgtgt
    15601 cgtcagtgct tgtgcgccga gcgtttgaaa tgtttgcgga gaagataacg cagcggtata
    15661 cagcaacaac gccagcgtcg tacttaacag agcgactaaa agccaggcca acgggccaag
    15721 caaccagcct ccaatacgtt gtggcgttgt ggtggtcata ggttctccag aatagcgacg
    15781 caaacaggca aatctaattt taggggcgtt attataaggc ttttataacc gatcgctact
    15841 ttctttattg gacatagtgc cagacggact tcggtacctg gccgaggtca aacaaacgcc
    15901 cgccagaaac cagctctgcg cgacggtgat cggcagcacg atacatattg atcagttcct
    15961 gatcgtccgt cagggtgtaa ttaagatggt cgtaaagttt ttccagactt tcgagtgaac
    16021 tgattttgcg aaattttaat aagtagtcct gaacagtcat aaaaggtcca taaaataatt
    16081 aataccaaag gggtaggtat tattagtgat gataaaattt caccaaaagt gaacgcttgt
    16141 gttattacaa taataagaat gcgctgatgg caaatattca gggatgaaaa actgctatga
    16201 aaagagtaac gcgctttact atccctgaca atcaccaaca acaccgaaca agataataaa
    16261 ttcctggttt aatatccgac aagtgaaaac atgcaccctg acgggcagca tgtcgctcta
    16321 caagcgcaga gcttacttgt gttgtaccgt agtactctgt tcaggtggct gatagttgtc
    16381 aatgtgactc gccacgccaa gaagaatgac tgaaacgaca agaacgatcc aacctgttaa
    16441 ttcaataaga cgattcatta caactcacat ctctgttgat tgatccatta acttcagggg
    16501 gtaaatgtta cttagcaata atagctccag caagattttt actgaggttt ttgcgatatt
    16561 tagcttttgt cgttggaaaa atcgctattt ttttgactta agttaaacaa catcccttat
    16621 tgctggcagg ttattaaact gttgagcgag ggtaaaggat agtgtcaaat agccatcata
    16681 cttcaatgag aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt
    16741 atgcgagcaa aaccttccat gcgcccctga ttgcgggcac gcccgggctg gaactggcgg
    16801 taatctccag cagtgatgaa acaaaagtaa aagccgactg gccaacggtt acggttgtct
    16861 ctgagccgaa gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca
    16921 acgataccca tttcccgtta gccaaagcag cgcttgaggc gggtaaacat gtggtcgttg
    16981 ataaaccctt taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc
    17041 tggggcgtgt gctgtctgta ttccataacc gtcgctggga tagcgatttc ttgacgctaa
    17101 aaggtttact cgcggaaggc gtgctgggtg aagtcgctta ctttgagtct cattttgacc
    17161 gcttccgtcc gcaggtgcgc gatcgttggc gtgaacaggg tggcccaggc agtggtatct
    17221 ggtacgattt agcaccgcat cttcttgatc aggctattac attatttggt ttaccggtca
    17281 gcatgacggt ggacttggca cagttacggc ctggagcgca gtcgaccgat tatttccacg
    17341 ccatcttgtc ctatccacag cggcgagtca ttttacacgg taccatgctg gcagccgctg
    17401 agtcagcacg gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc
    17461 cacaggaaga acgtctgaaa agtggcgagc gtctgccgca ggaagactgg ggctacgata
    17521 tgcgtgatgg cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga
    17581 cggtgcctgg gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg
    17641 gtgaaaatcc ggttccggca agccaggcaa tccaggtaat ggagttgatt gagctgggca
    17701 tcgaatccgc caaacatcgc gcgactttgt gccttgcgtg aaaatggatg gtgcaatttg
    17761 caccatccat ttttgttact tcgctgcgac tttttctcgc agtgcgcgtt tttcctcagc
    17821 gctgaggaaa gccatttcca gaccattaat ctgtgcctgg cggatttgct cgcgggataa
    17881 cccagcagct ggcgcggcaa cgttatattc gtgaatgata tccactccct gtacgccggg
    17941 atcgtcagtg ttaatgctgg cacgaatgcc atgatcaagg aacgttttca gcggatgtgc
    18001 agccagctca gccacggtgc tggtctgaat attggaggtc agacaggatt caataccaat
    18061 ttgttgctcg gcgagaaaat ccatcagcgc ccgatcttca atggctttta cgccatgtcc
    18121 aatacgctcc gcacccagtt cacgaatcgc ctgccagatg ctttccggcc cggcagcttc
    18181 gcctgcatgg acggtaatat gccagcccgc atcacgcgcg cggttgaagt gagaaagaaa
    18241 cagacttccc gggaaaccaa gttcatcacc ggctaaatca agtgcggtaa tctggtcacg
    18301 gtgggctaaa aaggcctcca gctcttgctg acaggcggct tcgccgaagg ttcggctcat
    18361 aatgccgata agcttcgcct gcacaccaaa ggtgcggcaa ccttcacgta cgccatcgat
    18421 caccgcttca acaacacctg caacaggcag ctgatgtgcc attgccatgt agcctggaga
    18481 aaaacgcagc tcgacatagt gcaggccgtg acgggctgca tcttcaatgt tttcaaatgc
    18541 cacgcggcga caagcatcaa gagaggcgag aactttaacg ccccagtcaa gtttggtcag
    18601 aaagctcacc agatcgggtt cgttggcaat gacctgaacg tggggaatca gtgtttccag
    18661 ggattgtgca ggaagcgaga tattatactg gcggccaagt tcaagaatgg tctggggacg
    18721 aatgttgcca tcaaggtggc gatgaatatc agttaatggc agggtggtat caatcatggt
    18781 cgcactcttt ttctggatat aaaagtgcgc accattataa aaacaaagca gggtaaaaat
    18841 ccacgattgc gcaacgattt tttacgttgc gcaattgggg ttagcgaaca gcgcggatgg
    18901 cgttaattaa tccagccaca cctttttcca gtttcgaacg tgggcagccg gcattgagtc
    18961 ggacaaaacc acgaccttct tcaccgtagg tataccccgg catgatcgcg actttttctt
    19021 gttcgataag tgctttttgc aaggcgttgt cgtcaatatt caacggacgt aaatcaagcc
    19081 aggccagata agtggattgc gggatctgcc agttgagttc aggaaacgtg gcgttcattt
    19141 tatctgcgat atacgtcagg ttatctttca gatagacgcg taaggcatcc agccacggtg
    19201 caccttgctg ataggcggcg atatgggcag ttaacgccag taccgaaggg gaagaaagcc
    19261 catcacgtcc tttcagtgcc gataaatagg catcacggct gctgctattt tctataatcc
    19321 cgtaagcacc ggtcagcgcg ggaatattga aacttttcga tcccgacgtc agcaacgccc
    19381 agtctccccg cgccacatta ctccagggaa tatgcggctg ctcgccccaa accatatcca
    19441 tatggatttc atcggaaata accctcacac catgacgctc gcacaggtca gccatgatct
    19501 ccagctcatc gcacgtccac acttttccgg taggattctg tgggctacac aggagcataa
    19561 ttttacattc tggtttcgcc aacacggctt ccagcttgcc catatcgcaa aaccagccgt
    19621 cagcctgctt ctctaaagca acgggcatta ctgtgcgctg attaccttca atggccttgt
    19681 aaaatgcgtc ataggcgggt gtgtggatca ccacgccttc acctgtttca gaccactgac
    19741 gaatcagttc tgagaccata tagataacag aagggccata caccaccgac tgaggatcga
    19801 tggcggtgta atgctgggtg gaaaaccagt gggcaatagc cgcgagaaac tcatcgttct
    19861 tccagcggct gtagccaaac acgccgtgca tcaggcgctg attcagcgcc tcgataatgc
    19921 agggggcagt ggcaaaatcc atgtctgaaa tcgtgaaagg taacaggtca gcagtgccga
    19981 aacggtcagc gacataatcc cactgcgtac accatgtgcc atgacgatcc acgacctttg
    20041 aaaaatcgaa catatcttat ccttatgcct ggacagtatg catcagaccg gccatttcat
    20101 cttttactga ctgaacttgt ggcccgataa caacctgcag gttatgttga ttaagttgta
    20161 ctacgccaat tgcccgattg tccttcagtg cctgcacatt aacaagcgac atatctttca
    20221 cagacaaacg caaacgggta atgcagttat caaggctgac aatattgtcg gcaccgccta
    20281 aggcttcgag gattgccgga acgttgtaac ctgatttacc cggcgcaccg gcaacggctt
    20341 tttcgattga actggccact tcgctatcgc gccccggggt tttcagattg aagcgggtga
    20401 tagcgaaacg gaagatgacg tagtaaacga caaaccagat tgccgccacc actggcacca
    20461 tgtaccactt ggttgacaga ccatgcaaaa taccgaacac cacgaagtcg atgatattgc
    20521 cgtcggtatt accgatggtg acgccgagca cagacatgac ggtgaagccg aggccggtta
    20581 acagcgcgtg gatgacatac agaactggcg ctacgaacag gaacaggaat tccagcggtt
    20641 cggtcgtgcc gccaacgaca caggcgatca ggccagaaat cagcagccct ttaattttat
    20701 ggcgattttc cgggcgcgcg cagtgataca tagctaacgc tgcacctggc agaccgccga
    20761 gaaacgcagg cattttgcct tgcgaaagga aacgcgtggc gctttcagaa aaaccgtgag
    20821 tggtcgggca actcaattgc gcctggaaga tggtcagtgc accgctgacg gtttgaccgc
    20881 agacttcctg cgtgccgcct gcgtcggtaa agcgaattaa tgccaccaga atgtgatgca
    20941 gaccaaacgg caacagcaga cgttcaccgg taccaaacag catcggtccg aaatcacccg
    21001 cgctattgat catatggcct aagccgctaa tacccatggc gaaaattggc cagactaatg
    21061 gaatcaccag accgacaatg cccatcacca gcgaggagat gattggtacg aagcgcgtac
    21121 cgccgaagaa tgccagcgca tccggcaggc ggatattatg gaatcgctca tgcagcatcc
    21181 agacgataat accggcgatc accgcaccga ggatcccggt gtcgatcgac tggatcccaa
    21241 gaatgctctg gatgttattg gctttcagaa cggcggcatc cgtggttggc agaatgcctt
    21301 tattggtcaa ccagaagttt accgcgaggt tcattaccgc ataaccgacg aagccagcga
    21361 atgccgctac gcctttgttt tcgcgtgcca ggcccagcgg gatggcaata cagaacatga
    21421 ctggcaggaa actaaaagca aacgagccaa tcttactcat ccaggtaaag atagcctgca
    21481 acacgggatt gcccaagacc gggatcaggg tgatgacatc gtggctacta agggaactac
    21541 caatgccgag cataatgccg cagaacgaca ataatgccac gggtaacatg aaggttttgc
    21601 ctaactgctg gaagaactcc cacagcgtga ctttcggtgc tgttttcgcc gtcataaaac
    21661 gactcctcgt agagaacaat ctggattcag taaattgcgc gagaagataa aacgttttat
    21721 caaattttag tgaggcataa atcacattac gcaacgataa tagcgggtat aagataaata
    21781 aaaggtaaaa cgttttatct gtcacaaaat cagggagtag gtcatctgca tggctaccgc
    21841 caaaaaaata accattcatg atgttgcgct ggctgcgggc gtgtcggtaa gtaccgtttc
    21901 gctggtgctt agtggcaaag ggcgaatctc taccgccaca ggagaacgcg ttaacgccgc
    21961 cattgaagag ctgggatttg tgcgcaatcg ccaggcgtcg gcgctgcgcg gcgggcaaag
    22021 cggcgtcatt ggtttgatcg tccgtgattt atctgcgccg ttttacgccg aattgacggc
    22081 cggattgacg gaagctctgg aagcgcaggg acggatggtt tttttgcttc acggcggtaa
    22141 agacggcgag cagctggcac agcggttttc actgttactg aatcagggtg tcgatggtgt
    22201 ggtaattgcc ggggctgcag gaagcagcga tgacctgcga cggatggcag aagaaaaagc
    22261 tatcccggtg attttcgctt cccgtgccag ttatcttgat gatgttgata cggttcgccc
    22321 ggacaacatg caggctgcac agttgttgac ggagcatctc attcgcaatg ggcatcagcg
    22381 gatcgcctgg ctgggagggc aaagttcctc attaacccgt gcagaacggg tggggggcta
    22441 ttgtgcaact ctactaaaat ttggcctgcc gtttcacagc gattgggtgt tggagtgcac
    22501 ttccagccag aagcaagccg cggaagctat cacggcgctt ttacgtcata acccgaccat
    22561 cagtgccgtg gtttgctata acgaaactat tgcgatgggg gcatggtttg gtttgctgaa
    22621 agcagggcgg caaagcgggg aaagcggagt cgatcgttac tttgagcaac aggtttcgct
    22681 ggcggcattt accgatgcga caccaaccac acttgatgat atacccgtta cctgggccag
    22741 cacgcctgcg cgggaacttg gtaccacact tgcggatcgc atgatgcaaa aaatcaccca
    22801 tgaagagacg cattcacgca atcttattat tcccgcccgg ctcattgcgg cgaaataatc
    22861 ctctctttat ctgctatacc tggtagtgtc ctttcctcaa ggttaatgat aaaaaatgtg
    22921 gcacacaggc ggtgcctttt atttttactg aagttcacag gaggtttatg tgtttaattc
    22981 tgacaacctg agactcgacg gaaaatgcgc catcatcaca ggtgcgggtg caggtattgg
    23041 taaagaaatc gccattacat tcgcgacagc tggcgcatct gtggtggtca gtgatattaa
    23101 cgccgacgca gctaaccatg ttgtagacga aattcaacaa ctgggtggtc aggcatttgc
    23161 ctgccgttgt gatattactt ccgaacagga actctctgca ctggcagact ttgctatcag
    23221 taagctgggt aaagttgata ttctggttaa caacgccggt ggcggtggac ctaaaccgtt
    23281 tgatatgcca atggcggatt ttcgccgtgc ttatgaactg aatgtgtttt cttttttcca
    23341 tctgtcacaa cttgttgcgc cagaaatgga aaaaaatggc ggtggcgtta ttcttaccat
    23401 cacttctatg gcggcagaaa ataaaaatat aaacatgact tcctatgcat catctaaagc
    23461 tgcggccagt catctggtca gaaatatggc atttgacctg ggtgaaaaaa atattcgggt
    23521 gaatggcatt gcgccggggg caatattaac cgatgccctg aaatccgtta ttacaccaga
    23581 aattgaacaa aaaatgttac agcacacgcc gatcagacgt ctgggccaac cgcaagatat
    23641 tgctaacgca gcgctgttcc tttgctcgcc tgcagccagc tgggtaagcg gacaaattct
    23701 caccgtctcc ggtggtgggg tacaggagct caattaatac actaacgaac cggtaaacag
    23761 ccgtatgtgt tgtttaccgg gatagcgtgt gcaattcatc aacgtctctg ctaaacaact
    23821 ggcagccaaa tcacggctat tggttaacca atagctggat gaaaagcata cgaatagagt
    23881 gtgccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg gtaaactagt
    23941 tattcacgct aactataatg gtttaatgat ggataacatg cagactgaag cacaaccgac
    24001 acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat ttcacagtgc
    24061 ctcgatgaaa gccatctgta aatcctgcgc cattagtccc gggacgctct atcaccattt
    24121 catctccaaa gaagccttga ttcaggcgat tatcttacag gaccaggaga gggcgctggc
    24181 ccgtttccgg gaaccgattg aagggattca ttttgttgac tatatggtcg agtctattgt
    24241 ctctctcacc catgaagcct ttgggcaacg ggcgctggtg gtcgaaatta tggcggaagg
    24301 gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga tcactgaatt
    24361 tgttgctcag cggatgcgtg atgcccagca aaaaggcgag ataagcccag acatcaacac
    24421 ggcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg atatcgaagc
    24481 ggaagacctg gcgcgtgaag cgtcgtttgc tcagggatta cgcgcgatga ttggcggtat
    24541 cttaaccgca tcctgattct ctctctcttt ccgcgggctg gtgacaactg agcccgcgtt
    24601 tcatacttta atttccctgt acaaaataca atctcgccgg agcggggaat tcctccctaa
    24661 aatattcact gtagccatat gtcatgagag tttatcgttc ccaatatgct cgaacgaacg
    24721 ttcgattgct tattttatgg cttctgtcaa cgccgtttta aagattaatg cgatctatat
    24781 cacgctgtgg gtattgcagt ttttggtttt ttgatcgcgg tgtcagttct ttttatttcc
    24841 atttctcttc catgggtttc tcacagataa ctgtgtgcaa cacagaattg gttaactaat
    24901 cggattaaag gttgaccagt atttttatct taatgaggag tcccttatgt tacgtcctgt
    24961 agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg
    25021 cgaaaactgt ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat
    25081 tgctgtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgtggg
    25141 caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt
    25201 gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat
    25261 ggagcatcag ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg
    25321 gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc
    25381 gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc atgatttctt
    25441 taactacgcc gggatccatc gcagcgtaat gctctacacc acgccgaaca cctgggtgga
    25501 cgatatcacc gtggtgacgc atgtcgcgca agactgtaac tacgcgtctg ttgactggca
    25561 ggtggtggcc aatggtgatg tcagcgttga actgcgtgat gcggatcaac aggtggttgc
    25621 aactggacaa ggcaccagcg ggactttgca agtggtgaat ccgcacctct ggcaaccggg
    25681 tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta
    25741 cccgctgcgc gtcggcatcc ggtcagtggc agtgaagggc gaacagttcc tgatcaacca
    25801 caaaccgttc tactttactg gctttggccg tcatgaagat gcggatttgc gcggcaaagg
    25861 attcgataac gtgctgatgg tgcacgatca cgcattaatg gactggattg gggccaactc
    25921 ctaccgtacc tcgcattacc cttatgccga agagatgctc gactgggcag atgaacatgg
    25981 catcgtggtg attgatgaaa ctgcagctgt tggctttaac ctctctttag gcattggttt
    26041 cgaagcgggc aacaagccga aagaactgta cagcgaagag gcagtcaacg gggaaactca
    26101 gcaggcgcac ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt
    26161 ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caaggtgcac gggaatattt
    26221 cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa
    26281 tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct
    26341 gaaccgttat tacggatggt atgtccaaag cggcgatttg gaaacggcag agaaggtact
    26401 ggaaaaagaa cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata
    26461 cggcgtggat acgttagccg ggctgcactc aatgtacacc gacatgtgga gtgaagagta
    26521 tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg
    26581 tgaacaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg
    26641 taacaagaag ggcatcttca cccgcgaccg caaaccgaag tcggcggctt ttctgctgca
    26701 aaaacgctgg actggcatga acttcggtga aaaaccgcag cagggaggca aacaatgaat
    26761 caacaactct cctggcgcac catcgttggc tacagcctcg gtgacgtcgc caataacttc
    26821 gccttcgcta tgggggcgct cttcctgcta agttactaca ccgacgtcgc tggcgtcggt
    26881 gccgctgcgg cgggcaccat gctgttactg gtgcgggtat tcgatgcctt cgccgacgtc
    26941 tttgccggac gagtggtgga cagtgtgaat acccgctggg gaaaattccg cccgttttta
    27001 ctcttcggta ctgcgccgtt aatgatcttc agcgtgctgg tattctgggt gccgaccgac
    27061 tggagccatg gtagcaaagt ggtgtatgca tatttgacct acatgggcct cgggctttgc
    27121 tacagcctgg tgaatattcc ttatggttca cttgctaccg cgatgaccca acaaccacaa
    27181 tcccgcgccc gtctgggcgc ggctcgtggg attgccgctt cattgacctt tgtctgcctg
    27241 gcatttctga taggaccgag cattaagaac tccagcccgg aagagatggt gtcggtatac
    27301 catttctgga cgattgtgct ggcgattgcc ggaatggtgc tttacttcat ctgcttcaaa
    27361 tcgacgcgtg agaatgtggt acgtatcgtg gcgcagccgt cattgaagat cagtctgcaa
    27421 accctgaaac ggaatcgccc gctgtttatg ttgtgcatcg gtgcgctgtg tgtgctgatt
    27481 tcgaccttcg cggtcagcgc ctcgtcgttg ttctacgtgc gctatgtgtt aaatgatacc
    27541 gggctgttca ctgtgctggt actggtgcaa aacctggttg gtactgtggc atcggcaccg
    27601 ttggtgccgg ggatggtcgc gaggatcggt aaaaagaata ccttcctgat tggcgctttg
    27661 ctgggaacct gcggttatct gctattcttc tgggtttccg tctggtcact gccggtggcg
    27721 ttggttgcgt tggccatcgc ttcaattggt cagggcgtta ccatgactgt tatgtgggcg
    27781 ctggaagctg ataccgtaga atacggtgaa tacctgaccg gcgtgcgaat tgaagggctc
    27841 acctattcac tattctcatt tacccgtaaa tgcggtcagg caatcggagg ttcaattcct
    27901 gcctttattt tggggttaag cggatatatc gccaatcagg tgcaaacgcc ggaagttatt
    27961 atgggcattc gcacatcaat tgccttagta ccttgcggat ttatgctact ggcattcgtt
    28021 attatctggt tttatccgct cacggataaa aaattcaaag aaatcgtggt tgaaattgat
    28081 aatcgtaaaa aagtgcagca gcaattaatc agcgatatca ctaattaata ttcaataaaa
    28141 ataatcagaa catcaaaggt gcaactatga gaaaaatagt ggccatggcc gttatttgcc
    28201 tgacggctgc ctctggcctt acctctgctt acgcggcgca actggctgac gatgaagcgg
    28261 gactacgcat cagactgaaa aacgaattgc gcagggctga taagcccagt gctggcgcgg
    28321 gaagagatat ttacgcatgg gtacagggag gattgctcga tttcaatagt ggttattatt
    28381 ccaatattat tggcgttgaa ggcggagcgt attatgttta taaattaggt gctcgtgctg
    28441 atatgagtac ccggtggtat cttgatggtg ataaaagttt tggctttgcc ctgggggcag
    28501 taaaaataaa acccagtgaa aatagcctgc ttaaattagg tcgcttcggg acggattata
    28561 gttatggtag cttaccttat cgtattccgt taatggctgg cagttcgcaa cgtacattac
    28621 cgacagtttc tgaaggagca ttaggttatt gggctttaac gccaaatatt gatctgtggg
    28681 gaatgtggcg ttcacgagta tttttatgga ctgattcaac aaccggtatt cgtgatgaag
    28741 gggtgtataa cagccagacg ggaaaatacg ataaacatcg cgcacgttct tttttagccg
    28801 ccagttggca tgatgatacc agtcgctatt ctctgggggc atcggtacag aaagatgttt
    28861 ccaatcagat acaaagtatt ctcgagaaaa gcataccgct cgacccgaat tatacgttga
    28921 aaggggagtt gctcggcttt tacgcgcagc tcgaaggttt aagtcgtaat accagccagc
    28981 ccaatgaaac ggcgttggtt agtggacaat tgacctggaa tgcgccgtgg ggaagcgtat
    29041 ttggcagtgg tggttatttg cgccatgcaa tgaatggtgc cgtggtggat accgacattg
    29101 gctatccctt ttcattaagt cttgatcgta accgtgaagg aatgcagtcc tggcaattgg
    29161 gcgtcaacta tcgtttaacc ccgcaattta cgctgacatt tgcaccgatt gtgactcgcg
    29221 gctatgaatc cagtaaacga gatgtgcgga ttgaaggcgc gggtatctta ggtggtatga
    29281 actatcgggt cagcgaaggg ccgttacagg ggatgaattt ctttcttgct gccgataaag
    29341 ggcgggaaaa gcgcgatggc agtacgctgg gcgatcgcct gaattactgg gatgtgaaaa
    29401 tgagtattca gtatgacttt atgctgaagt aagaaataac gccggagaga aaaacctccg
    29461 gcgttttggc tgtgaattac tgctgcggaa tagcgggtac gaccggaacg tttaatgccg
    29521 gcattgcaaa cataccaacg aaatcttcca gtggcatttt ctgcccattt aacgttatct
    29581 gaccgttagc atattgcagg ctggtggtga tggtattgtc ctgcaaggtg gtcagacgga
    29641 acatctgccc cattgctgat gcaccttcaa cttgctgttt cgccagtttt ttcgcttgat
    29701 cttcctgata accttccagc ttcgctacct gagtcatcaa ctcagttgcc atatccaccg
    29761 gaatggtcag tttcgcatcc agagatttaa ccgaacgatc tacttcctgc gccagcgttt
    29821 gcggcgcttc tttagtcgtt gccgggtctt tcaggaacag cgacagattc agcgcacttt
    29881 caccctgact gtttttccag cttagcggcg cgatagtaat caccggatcg cctttcaaca
    29941 tcagcggcag ggcgctaaag aaggcttccg tcactttctc ctgataaagt tcgggattgt
    30001 tggcaatttc tggctgtgcc agcagcgcct gagtttgcgc gttatattgc tggctaaact
    30061 gatgccaggc ttcaccatca atctggccga cttttaaagt cagcttgccg ctgcccagat
    30121 cctgattctg taccttcagg ctgtttagcg agtaatccag ttggctattg atcgttttac
    30181 cgtcattgac cagatccgat ttaccgctga tctccatgcc ttccagcagt gccagttctt
    30241 tgccttccac tgaaatggtc attttttcca gtgacagttt ttggtttcct acgcgctcac
    30301 caaaacttgc cagcgtgctg gaaccgtcgg ttttcagatt attaaaggtc aactgtactt
    30361 tctggttgta ttcgttaacc gcgtctatcc gaccactttg cgcctccccg gaaagggaga
    30421 tagctttgcc gtctctgtcc gcatttaact ggaactcgcc gccgctaaag gcgacttttt
    30481 catccttttg ctcgtaattc agcggcttga gcgaaatatc ggaactggaa tcaccgctgt
    30541 aaccaatgcg cgagttaatc tcaaaaggcg tttcaccttt tgccatatca aacagtggtt
    30601 tgcttacttc gttattaacc agcgtggttt gaattgatgc catcgatggg atcaggttca
    30661 gttttttaag ctgggcaagg gggaagggac catgatcaac cgattcgttg aagatgacgc
    30721 tctgaccgct tttaatccac ggattttctt tcccggcaat gggtttcacc aacagttgca
    30781 actggctgct gaatacgccg cgatgatagt tttgataact cacttccagg ttggattcag
    30841 gagctgtcag tttgagttgc gcgttcgcct gcgcgaccat ctcttcgaga tgggtttcaa
    30901 tcttcttgcc tgtataccat gcgccgcctg tccagactac gcctagcgca acaatgacgc
    30961 ctaccgctac cagcgattta ttcataatga ttatccataa aatgaaatca ggcggactgg
    31021 ccgcctgaag gtgttataag cctttaataa gcttagcaag agatgttaat tttttcagta
    31081 agctcttaca gcttgttgta aacacgcgct aaacggccgt ggcctttgac agtcaccggt
    31141 gattcgttgg cggcaataaa cgctgattca cccggtttaa gctgtaactg ctgagaacct
    31201 ttccacaacg ttgcatcgcc ttcgacgcag aacaaaatgg cggcactctg ctggctaatg
    31261 gtggtttctt tatcactaag gtcatgcagc gagaaggcaa aatcatcaac aggaatcggg
    31321 aagtccagtt ctgcaccttg tttcaccggc tgggtcaaca actggttagc cggtttggct
    31381 tcgaacttca cattggcaac cagttccgga atatcaatgt atttaggcgt cagacccgca
    31441 cgcagcacgt tatcggagtt tgccatcact tccagcgcca cgccttgcag gtaagcgtgc
    31501 ggtgtttcag cgaacaggaa catcgcttcg ccaggattca atttcaccac attcagcaac
    31561 agcggagaga acaggccgct gtcatccggg taaaattcag aaattaaaag aatcgtttgc
    31621 cacggttcac cctgctgact gtcgagcgcc gattttaaaa tcgccagcgc gcgggatttt
    31681 tcttcaccct gcatattcaa caggctggcg aacagttcgc ttaaacgttc ggcatcaggc
    31741 tgttgtaaaa agtgagcaat cgccggatgt gcacctgcga ccggctggag tagggagaca
    31801 atctcggaaa attcacgaaa cgcgttcatc gcaaggaaag gcgtcagcgc aaaaaccagc
    31861 tcaggcttgt ggttaggatc tttatagtta cgctcggcgg catccatcgg gatacctgcg
    31921 gcattttctt tggcaaaacc gatttcagaa ttgtgtttgt ttggatgaac ctgaatggag
    31981 agcggctgtg ctgcgcataa tactttgaac aggaaaggca gttcgccaaa gcgtttggca
    32041 acggcctctc cgagcagagt cgatttatca ctctcaatca catcacgcag tgaaacgata
    32101 tctccggcgg cattctgcac tcgtgaactg cttttcggat gtgcgcccat ccacagctcg
    32161 gccatcggct ggctggacgg attttccata ccataaagtt cagtcaacgc cgttttgctg
    32221 ccccaggcat agttttgcac tgagttaatg agtttttgca tgatcaatcc ctgttttaat
    32281 gtggaaatta atcccgctat taaagcaaga atcctacggg aagtaacctg gagccgcaaa
    32341 aagtcgtact agtctcagtt tttgttaaaa aagtgtgtag gatattgtta ctcgctttta
    32401 acagggcaac ggaacacccg cccagagcat aaccaaacca ggcagtaagt gagagaacaa
    32461 tgtcaaacaa accctttcat tatcaggctc cttttccact caaaaaagat gatactgagt
    32521 attacctgct aaccagcgaa cacgttagcg tatctgaatt tgaagggcag gagattttga
    32581 aagtcgcacc cgaagcgtta actctgttgg cgcgtcaggc gtttcatgat gcgtcgttta
    32641 tgctgcgtcc ggcgcaccaa caacaggtgg ccgacattct gcgtgacccg gaggccagcg
    32701 aaaatgataa atatgtggcg ctgcaattcc tgcgtaactc cgacatcgcg gcgaaaggcg
    32761 ttctgccaac ctgtcaggat accggcaccg cgattattgt tggtaaaaaa gggcagcgtg
    32821 tatggaccgg tggtggtgat gaagcggcgc tggcgcgcgg tgtctataac acttatatcg
    32881 aagataatct gcgctactcg caaaacgcgc cgctggatat gtataaagaa gtgaataccg
    32941 gcaccaatct accagcgcag atcgatcttt atgccgttga tggcgacgag tacaaattcc
    33001 tctgtatcgc caaaggtggt ggttcggcaa acaagacgta tctctatcag gaaaccaaag
    33061 cgttactgac gccggggaaa ctgaaaaatt acctggttga gaagatgcgc acgctgggta
    33121 cggcggcctg tcctccgtat catattgcgt tcgttattgg tggaacttct gcagaaacga
    33181 accttaaaac ggtgaaactg gcttccgcga aatactatga tgaactgcca acggaaggga
    33241 atgagcacgg tcaggcgttc cgcgatgtgg aactggaaaa agaattgctg atcgaagcgc
    33301 aaaatcttgg tctgggtgcg cagtttggtg gtaaatactt cgctcacgac atccgcgtga
    33361 ttcgcctgcc acgtcacggc gcatcctgcc cggtcggtat gggcgtctcc tgctctgctg
    33421 accgtaatat caaagcgaag atcaaccgtc aggggatctg gatcgaaaaa ctggaacata
    33481 atccaggcaa atatatcccg gaagagctgc gcaaagcggg agaaggcgaa gcggtgcgcg
    33541 ttgaccttaa ccgtccgatg aaagagatcc tcgcacagtt gtcgcagtat cccgtttcta
    33601 cacgcttatc gcttaacggc acgattatcg ttggtcgtga tattgcccac gccaaactga
    33661 aagagcggat ggataacggt gaagggctgc cgcagtacat taaagatcat ccgatttact
    33721 acgcgggtcc ggccaaaacg ccggaaggtt atgcctccgg ttctcttggc ccaacgaccg
    33781 ccggacggat ggattcttat gtcgatcaac tgcaagcgca gggcggaagt atgatcatgc
    33841 tggcgaaagg caaccgcagc cagcaggtga cggatgcctg taaaaaacac ggcggcttct
    33901 accttggcag tatcggtggt ccggccgctg tattggcgca gggaagtatt aagagcctgg
    33961 aatgtgttga atatccggaa ctgggaatgg aagccatctg gaaaattgaa gtggaagatt
    34021 tcccggcgtt tattcttgtg gatgataaag gaaatgactt ctttcagcag atacaactca
    34081 cacaatgcac ccgctgtgtg aaataaacag agccgccctt cggggcggtt tttttacatg
    34141 gcacgaaaga ccaaacattt gttatcaaat ggtaaataat aagtgagcta aaagttgctt
    34201 aacgaaagca aaacagaaag aaaaaattaa tcaggtgagg agcaggccat gaatacagta
    34261 cgcagcgaaa aagattcgat gggggcgatt gatgtcccgg cagataagct gtggggcgca
    34321 caaactcaac gctcgctgga gcatttccgc atttcgacgg agaaaatgcc cacctcactg
    34381 attcatgcgc tggcgctaac caagcgtgca gcggcaaaag ttaatgaaga tttaggcttg
    34441 ttgtctgaag agaaagcgag cgccattcgt caggcggcgg atgaagtact ggcaggacag
    34501 catgacgacg aattcccgct ggctatctgg cagaccggct ccggcacgca aagtaacatg
    34561 aacatgaacg aagtgctggc taaccgggcc agtgaattac tcggcggtgt gcgcgggatg
    34621 gaacgtaaag ttcaccctaa cgatgacgtg aacaaaagcc aaagttccaa cgatgtcttt
    34681 ccgacggcga tgcacgttgc ggcgctgctg gcgctgcgca agcaactcat tccgcagctt
    34741 aaaaccctga cacagacact gaatgagaaa tcccgtgcat ttgccgatat cgtcaaaatt
    34801 ggtcgtactc acttgcagga tgccacgccg ttaacgctgg ggcaggagat ttccggctgg
    34861 gtagcgatgc tcgagcataa tctcaaacat atcgaataca gcctgcctca cgtagcggaa
    34921 ctggctcttg gcggtacagc ggtgggtact ggactaaata cccatccgga gtatgcgcgt
    34981 cgcgtagcag atgaactggc agtcattacc tgtgcaccgt ttgttaccgc gccgaacaaa
    35041 tttgaagcgc tggcgacctg tgatgccctg gttcaggcgc acggcgcgtt gaaagggttg
    35101 gctgcgtcac tgatgaaaat cgccaatgat gtccgctggc tggcctccgg cccgcgctgc
    35161 ggaattggtg aaatctcaat cccggaaaat gagccgggca gctcaatcat gccagggaaa
    35221 gtgaacccaa cacagtgtga ggcattaacc atgctctgct gtcaggtgat ggggaacgac
    35281 gtggcgatca acatgggtgg cgcttccggt aactttgaac tgaacgtctt ccgtccgatg
    35341 gtgatccata atttcctgca atcggtgcgc ttgctggcag atggcatgga aagttttaac
    35401 aaacactgcg cagtgggtat tgaaccgaat cgtgagcgaa tcaatcaatt actcaatgaa
    35461 tcgctgatgc tggtgactgc gcttaacacc cacattggtt atgacaaagc cgcggagatc
    35521 gccaaaaaag cgcataaaga agggctgacc ttaaaagctg cggcccttgc gctggggtat
    35581 cttagcgaag ccgagtttga cagctgggta cggccagaac agatggtcgg cagtatgaaa
    35641 gccgggcgtt aatctgcaac atacaggtgc agccgtggaa tgatcaaacg aagcggctgc
    35701 gcctgaggtt tataacgatg ctgcacattg tcggcatcgt aatttaacaa ctcaccaacg
    35761 tccggcacgc ccgcaccatt atcccgatta atcagtgcaa tcagcggtgt agggcaggcg
    35821 tgttgcactt gtttttgatc tcctttgtac cagacgcggg caatcggctg caccttcacc
    35881 ggacgtttga tttttaactt cgcgttctgt ggcagggcag cgatatcctg atactctcgc
    35941 tccagttttc tttgccactc ctcgcgcgtc cacggtgcga cactgcgtgg tgatttcagg
    36001 cttttttcca gctgtgccag gacttcatca cgatgcaaat ttttaatgat atgtttatta
    36061 gcccagccaa aacgtaaagt ggcggggttg tgcagaacgg tgagcgtgcg gtaagcatta
    36121 agggtgatca gccccggcaa atgacgatgc acccattcaa aacgtgccgc ggtggggagt
    36181 tctgattcaa ccgtgacgat atgctcgaac gtggtcttga gtttattgat gtgctgaata
    36241 tgactgacca acgctgcttg cgaaaggtta tcgacctggt aacacaacac gccaggcaga
    36301 cggacagcgg ctttgctgct gcgattttcg gactgttgtt gaataaataa atgacggaaa
    36361 tgacgcaacg ccagcgactg cgcgtcgttg ccgagatgtt gtttcacctc aatacgatta
    36421 agcggattat gctcatcctc tttttttacc tccggcaaag agaacacgcg ggcaaccaat
    36481 agcttgtgtt gctcaagatg agcggcaaat gcagccagct cttgttccat ctggcgaaaa
    36541 gtagtgttga gtcggtctac gagatcgtaa cgcgccataa tattaaccac tttagttaca
    36601 acatccttaa tttatagcac agtggtggtg acttgaccac cgtgcgcgta gtgtcaagca
    36661 gaggtaaatt gcgggatgtt atgccataac ggccagctaa acgagaagcg ggcaccaccc
    36721 agttcgctgg tgtcacaatt aaccgtaccg cccattgcca gtgctataga gtggacaatt
    36781 gccagcccca gcccgcagcc gccggttgag cgatcccggc tgggatcgag gcgaacaaaa
    36841 ggttcaaaga tatgttcgcg gttttctggg gcaatccctg ggccatcatc ctcaacaatt
    36901 aatgtcgctc tattccccga cagtagcagg ctggtttcaa ccgttgaatg gcagtagcgc
    36961 agggcgttat tgagcaaatt atccagcacg cgctccatta agcgcatatc caacgccgca
    37021 taatggcctt gcatgagcgt ttttatccgt accgttttat cgggcgttac tgcctgaata
    37081 tctgccagat gcgttgatag ccacaacggc aggtctggtt cgctaagatg aagctcgttt
    37141 tgtgggcgat cgagtcgggc ataagtcagc agctcttcaa ttaaagcttc aagttgactg
    37201 atatcacgat tcaacgcctg ggattcggcg gcgctcaggt tatcgctcat ctccagtcga
    37261 taacgcaggc gcactaacgg tgttcgcagt tcgtgagcga taccgtcaat aagctgtttt
    37321 ttgctggcaa tcaaggcgtt gatattgtcc gccatctggt taaatgcgat accaagtcgt
    37381 tcaaagctcg aaccctcatc aaagtggata cgttcattga gatgcccatc gccaaatcgt
    37441 tgcgccgctg cttccagttt taacatatcc tgccagtgcg gacgcatcca gataaacacc
    37501 ggaaaggcga gggaaatagc aataaaagcg atcagggcga tatccagcaa tcgcatctga
    37561 tggaggtaat aaagataagg aacaggacca actgccagca cgtagtggct gcgcgggata
    37621 cgctgcaaaa acgtgtactg atcgtccagg gcgacaattt cgccgccacg cagtcggtgc
    37681 atggaaatat catcaagatg gtatttactc agtggctcga cacgcagatc gaaagagaga
    37741 tttaaatcca tctctttcag cgtcttaccc cagtcgtgtg gggggatctc acgcaattcg
    37801 ctgcgcatca gatacagcga actgttcatc aaatcatcca gcgactgttt gcccgcgcgt
    37861 tcggcggtaa atttgtacac cagcccaacc agcagagaca ttacaaggaa gcagacaaac
    37921 aataataggt aaaactggat aaacagtttt ttcatcgctt attcccatgc atgaggcgca
    37981 aagagatagc ctttgttacg cacagtttta atgcgataag gttctgcggc gttatcgagc
    38041 agtttttttc ttaaccgcga aatagccacg tccacgctac gatccaggcc gtcataactg
    38101 acgccgcgta aatttttcag caatgcatcg cggtccatga tttgcccggc atgggtagct
    38161 aattcccaca ataattcgaa atcagctgtc gagagcgaga tttcagtgtt agccagggtg
    38221 actacgcggt tgatgggatc gatggtcaac gtgccgaaat gcagggcttt gtagggagtc
    38281 agagacgttt cctgaagacc tttggtcagt gtggcttgct cattctgacg caaatgcaaa
    38341 cgtaaacgcg ctagcaaaac agcagggggc gtcgttttga gaatatagtc gcaggcaccc
    38401 atttccagtg ccaggatgtg gttcatatcg ctatcgagag aggttagaag aacaatcggt
    38461 ccagaccact ttgcgcgtaa atcacgacaa atggtcatgc cgtccttgcc tggtagcatg
    38521 atgtcgagta acaccaaatc cggattttct cgcaaaatgg tttcttcggc ctggtcgccg
    38581 cgcggctcta cggtaacctg catatcatgt tttgctaggt acgcggcaat cagtgaaccg
    38641 acttccgcat catcttccac aaatacgata gtgttcataa cattcacggt agatataaaa
    38701 acgtcacaat acaccgcgct gtttttactt actatcaatc gtttctaaac aagttttcat
    38761 caggtattct actgatgact tgttatggaa gtgttaaggt aaaaagatgg ggctggtaat
    38821 caaagccgcg ctcggtgcgc tggtagtgct gttgattggt gttttagcaa aaacgaaaaa
    38881 ttattatatc gccgggctga ttccactttt cccgaccttt gcgctcatcg cgcattatat
    38941 tgttgccagc gaacgcggca ttgaagcctt acgcgcaacc atcattttta gtatgtggtc
    39001 gattattccc tattttgttt acctggtgtc gctgtggtat ttcaccggaa tgatgcgctt
    39061 gcccgccgct tttgttggtt ctgttgcgtg ttgggggata agcgcatggg tgttgattat
    39121 atgctggata aagctgcatt aacgcagatg acgaccgcca tcaagtggga aactgcgtcc
    39181 ggtgacaaag caactggtaa gtaagtaatc gaccaggtcg atcacttctt tctcgccagg
    39241 cgcggttttc atcagtgatt tattcagcgc ctgttgtcga tattcggcat catcatgttc
    39301 attaaacagg atcagcgatg gcgcaataga attcactttc acttccggtg ccagcttgcg
    39361 ggcaaacgag cgggtcatat tatccagtgc cgctttgctt gcagcatacg caatatgctt
    39421 gtcgctaccg cgctccacca cataatcggt aaagtgaatg atatcgctgg cggcgtgtcc
    39481 gtgcccacgc agtaatcttt ccagcgcatg gttgagcagg tatggggtat taacgtggat
    39541 ctgcatcatg caagccagta cgtcggccag tggcgcaccc ggtttttccg ccatccacgc
    39601 actggcgtta tgcaaaatag cacgcagacc atgggtgctt tttagtactt catcggcaaa
    39661 cgccatcaca ccgtcgttgg tcgaaaaatc agcctgaata cactgcgcac ctgcattaat
    39721 cagtccatca atggctggat agtgtgtccg atagctgaca atcaccggtt gcttttgatt
    39781 aatgaaatgc catgcaaggg cgaggccgat gcgacgacct ccgccagtaa ttaatattgg
    39841 caagggctgg gttttaccca tcgttatctc ctttactatc caacgatggg atgaacgtta
    39901 tcccaccagc atccaggtcg ccggaactga ggcaattaac agcatgccaa tcagcaccat
    39961 ttcctgacgg ttcagtacat tatcatgtgt atgcgttttg cgcgcataca ggaaaaccag
    40021 cagccccggt gcatacagaa caacggataa cagcaagtgc attggaccag aggcgtacaa
    40081 taaccataag ccataaatgc aggcaccgac acctaccgct ttatgcagtg gacgggttgc
    40141 gattttcagc aagaacgcgc cgaccaggaa atagggcacc agaatcattt ctgaggcgat
    40201 ggtcagcaac gtgttgtaat cggaaccggt cagccagatg agtaccagac aaatttgcac
    40261 gcagatgttg gtcaaccaca gtgaggcgga tggcgcagct tgtgcgttct ggcgcgcaaa
    40321 aatgcgtggg aatgctttat gagtggcagc caggaacgga acttctgccg ccatgatggt
    40381 ccagcttaag tacgcgccgc aaacggaaac gatcaaaccg gcagcgatga tgatttcgcc
    40441 ccatggtccc atcatttcca ccatcagacc ggccattgat gggttacgaa tttcagccag
    40501 ttcaggacgt gccaccacac ccagcgaaag cagcgttacc agcaagtaaa cgcccagagc
    40561 ggagagaacc gccagtagtg tcgctttgcc aacatcacgt ttattacgcg cacgcgcaga
    40621 aacaaccacg gcaccttcca caccaatgaa aacccacagg gtgatcagca tggtgttttt
    40681 cacctgttcc caaacaggta cgccaagtgc aagtccggtg aagtcgagct tgaaggtatc
    40741 cagtttgaac atcatcatcg ccagcacaac aaacagaccc agcggcaaca attttgccag
    40801 tgtcgccacc aggttaatgc tggcagcggt ttgcaccccg cgcaggatca aaaagtgaac
    40861 aatccacaat aaagccgatg caccgactat cgattgccag gtattaccat cgccaaacag
    40921 gcgcaattcc ggcgtgtccg taaaaaagct taacgcggaa aaaacgatca ccagatagga
    40981 gacgttggcg atgaccgcgc acagccagta tccccatgcg gaacaaaagc cgattagctc
    41041 gccaaaccct tcgcgggcat aggtaaagat accgccgtca agttcgggac gaatgcgcgt
    41101 gaggatcagc atggcaaagg ccagcaataa aatgccagcg ccagtaatac cccagccgat
    41161 gagcagtgct gccgggctgg caactgccgc catattttgc ggcagactga aaacacccgc
    41221 gcccagcatt gagcttaata ccagcgcggt gagtgcgctc agtcccagtt tcttttccat
    41281 cgatatccag tgattaaaac gtgataagaa gaataaatat tagggcaaaa cgcataaaaa
    41341 tggagtttgc cactgaagcg cgggatttta cgaagagatg tgtctgcatg caatggtcgc
    41401 agggaaattt gtgactaaaa atgcgaaagg cggcatcaag ccgccttata ggagtaactg
    41461 aatagttatt tatacagatc tgcgctgacg gtcaggttat taccacgttc ctgccactgg
    41521 cgggtgatgt ggtaatactt agcacctttc ttcgcggcac gtttcgcaac ctgataggag
    41581 acttcggtca tgttgccgta gttgccagag aatttgatgc tgtcgaacgg gaccatcatc
    41641 gctgcggtcg ctttgttcag ttcttcgact ttagtgccat ccgggagcgt gacggtgtaa
    41701 cgcccgcctt ttgatgactg ggtttcaaag aagcgaccga cttcagaact tggtgatgcg
    41761 gtagtcgcaa cacccgggat ctcaactttc ttcgcggctt cgccaccggc agccagagct
    41821 gcacgtcctg cttcggaatc tgccgggatc acatccgggc tctggacgat acgtttctta
    41881 gcatcttttt tatagatgaa tgcagtaata cgctggttgc cgccctggtt ggcatcgatt
    41941 tgacgaacga tgtagaaaga gtaggcacct ttcgcttttg ccgctttggt gatggcatca
    42001 ttgacttctg gctggctacg atagaagccc tggacggtga ccgtgtcaaa cggttcaatc
    42061 agaacagcct gatctttcgg cagttcgaca acaccgttaa ttacgcgatt acttgtttct
    42121 tctgcttttt cagcatcggc tttatagagg tcagcgacca cacgccagtt accgctgtta
    42181 ccaaaatcag aagtgtcgac aacataaaaa gaggcggcac cttctttatc tgcgcgacga
    42241 gaaacggctt tcaccgcttc gccaatagca ttaaaacgac cggtaaccac tacacggtca
    42301 aaaggtttaa ccgctgccgc ttgctccggt gtcagttctg ttgctgcgtt aacggagaat
    42361 gccgtagcag aaagcagtgc cgacgccagg agggtgttct taagcttcat aaaaataatc
    42421 cttcgccttg cgcaaaccag gtactggtat tgttattaac gagaaacgtg gctgattatt
    42481 gcatttaaac ggtgtaactg tctgcgtcat ttttcatatc acattcctta agccattttt
    42541 aatcctgctc aaatgaccgt ctatgcttaa aaaacagccg tatcagcatc attactactg
    42601 aagcaactga attgtataag ttaatttaat gttaagtagt gattcgtgcc ggggcgatgt
    42661 ctcgttttac ccgaccgtcg aagacaatta tcagtcttta tccggcgttc taaggtgttt
    42721 atcccactat cacggctgaa tcgttaatat tttgcgagtt cacgccgaaa tactgatttt
    42781 tggcgctaga tcacaggcat aattttcagt acgttatagg gcgtttgtta ctaatttatt
    42841 ttaacggagt aacatttagc tcgtacatga gcggcttgtg tggctcctga cacaggcaaa
    42901 ccatcatcaa taaaaccgat ggaagggaat atcatgcgaa ttggcatacc aagagaacgg
    42961 ttaaccaatg aaacccgtgt tgcagcaacg ccaaaaacag tggaacagct gctgaaactg
    43021 ggttttaccg tcgcggtaga gagcggcgcg ggtcaactgg caagttttga cgataaagcg
    43081 tttgtgcaag cgggcgctga aattgtagaa gggaatagcg tctggcagtc agagatcatt
    43141 ctgaaggtca atgcgccgtt agatgatgaa attgcgttac tgaatcctgg gacaacgctg
    43201 gtgagtttta tctggcctgc gcagaatccg gaattaatgc aaaaacttgc ggaacgtaac
    43261 gtgaccgtga tggcgatgga ctctgtaccg cgtatctcac gcgcacaatc gctggacgca
    43321 ttaagctcga tggcgaacat cgccggttat cgcgccattg ttgaagcggc acatgaattt
    43381 gggcgcttct ttaccgggca aatcactgcg gccgggaaag tgccaccggc aaaagtgatg
    43441 gtgattggtg cgggtgttgc aggtctggcc gccattggcg cagcaaacag tctcggcgcg
    43501 attgtgcgtg cattcgacac ccgcccggaa gtgaaagaac aagttcaaag tatgggcgcg
    43561 gaattcctcg agctggattt taaagaggaa gcgggtagcg gcgatggcta tgccaaagtg
    43621 atgtcggacg cgttcatcaa agcggaaatg gaactctttg ccgcccaggc aaaagaggtc
    43681 gatatcattg tcaccaccgc gcttattcca ggcaaaccag cgccgaagct aattacccgt
    43741 gaaatggttg actccatgaa ggcgggcagt gtgattgtcg acctggcagc ccaaaacggc
    43801 ggcaactgtg aatacaccgt gccgggtgaa atcttcacta cggaaaatgg tgtcaaagtg
    43861 attggctata ccgatcttcc gggccgtctg ccgacgcaat cctcacagct ttacggtact
    43921 aacctcgtta atctgctgaa actgttgtgc aaagagaaag acggcaatat cactgttgat
    43981 tttgatgatg tggtgattcg cggcgtgacc gtgatccgtg cgggcgaaat tacctggccg
    44041 gcaccgccga ttcaggtatc agctcagccg caggcggcac aaaaagcggc accggaagtg
    44101 aaaactgagg aaaaatgtgc ctgttcaccg tggcgtaaat acgcgttgat ggcgctggca
    44161 atcatccttt tcggctggct ggcaagcgtt gcgccgaaag agtttcttgg gcacttcacc
    44221 gttttcgcgc tggcctgcgt tgtcggttat tacgtggtgt ggaatgtatc gcacgcgctg
    44281 catacaccgc tgatgtcggt caccaacgcg atttcaggga ttattgttgt cggagcactg
    44341 ttgcagattg gccagggcgg ctgggttagc ttccttagtt ttatcgcggt gcttatagcc
    44401 agcattaata ttttcggtgg cttcaccgtg actcagcgca tgctgaaaat gttccgcaaa
    44461 aattaagggg taacatatgt ctggaggatt agttacagct gcatacattg ttgccgcgat
    44521 cctgtttatc ttcagtctgg ccgggctttc gaaacatgaa acgtctcgcc agggtaacaa
    44581 cttcggtatc gccgggatgg cgattgcgct aatcgccacc atttttggac cggatacggg
    44641 taacgttggc tggatcttgc tggcgatggt cattggtggg gcaattggta tccgtctggc
    44701 gaagaaagtt gaaatgaccg aaatgccaga actggtggcg atcctgcata gcttcgtggg
    44761 tctggcggca gtgctggttg gctttaacag ctatctgcat catgacgcgg gaatggcacc
    44821 gattctggtc aatattcacc tgacggaagt gttcctcggt atcttcatcg gggcggtaac
    44881 gttcacgggt tcggtggtgg cgttcggcaa actgtgtggc aagatttcgt ctaaaccgtt
    44941 gatgctgcca aaccgtcaca aaatgaacct ggcggctctg gtcgtttcct tcctgctgct
    45001 gattgtattt gttcgcacgg acagcgtcgg cctgcaagtg ctggcattgc tgataatgac
    45061 cgccattgcg ctggtattcg gctggcattt agtcgcctcc atcggtggtg cagatatgcc
    45121 agtggtggtg tcgatgctga actcgtactc cggctgggcg gctgcggctg cgggctttat
    45181 gctcagcaac gacctgctga ttgtgaccgg tgcgctggtc ggttcttcgg gggctatcct
    45241 ttcttacatt atgtgtaagg cgatgaaccg ttcctttatc agcgttattg cgggtggttt
    45301 cggcaccgac ggctcttcta ctggcgatga tcaggaagtg ggtgagcacc gcgaaatcac
    45361 cgcagaagag acagcggaac tgctgaaaaa ctcccattca gtgatcatta ctccggggta
    45421 cggcatggca gtcgcgcagg cgcaatatcc tgtcgctgaa attaccgaga aactgcgcgc
    45481 tcgtggtatc aacgtgcgtt tcggtatcca cccggttgcg gggcgtttgc ctggacatat
    45541 gaacgtattg ctggctgaag caaaagtacc gtatgacatc gtgctggaaa tggacgagat
    45601 caacgatgac tttgctgata ccgataccgt actggtgatt ggtgctaacg atacggttaa
    45661 cccggcggcg caggatgatc cgaagagtcc gattgctggt atgcctgtgc tggaagtgtg
    45721 gaaagcgcag aacgtgattg tctttaaacg ttcgatgaac actggctatg ctggtgtgca
    45781 aaacccgctg ttcttcaagg aaaacaccca catgctgttt ggtgacgcca aagccagcgt
    45841 ggatgcaatc ctgaaagctc tgtaaccctg acggcctctg cggaggccgt cactctttat
    45901 tgagatcgct taacagaacg gcgatgcttt gacctcccgc tgtttgttca agcgcaattt
    45961 tgacaataat tgttaacggc acggaaagca gcatacccac cggtcctaac aaccatcccc
    46021 aaaaaatcaa cgacaaaaat accaccaatg tggaaagtcc cagcccacgc cccatgatgc
    46081 gtggctccag aatattgccg aagaccagat taatcagcag atatcccgcc agcaccaaca
    46141 acgcttcgta gaagccatta aacaccagta cctgagcgat aggggggatt gccgcgagaa
    46201 ctgaaccaat attagggatg taattaagcg caaaggccag caatccccag acaaaagcga
    46261 agcgaacatc gagggcggcg agcatcgccc atgcgaccag gccggtgatg atgctgatgg
    46321 ctgttttcag caccagataa tgagacacac tgtcaatcgc acgctgaatc gccgccatcc
    46381 cttcaaccgg acgcgccatc atttgctgaa attttccggg caattgtggc acttcgagca
    46441 gcataaacag caccgtcagc agcaataaaa atattgatga catggcatta gataactgcg
    46501 tcaataagtt ggtgagcaac gtcatcgccg cgttcggatc aatataatgc gccagctggt
    46561 caactgagac gtcaatccct acgcgttgca acaacggttc aagcgcttgc agcggcgtca
    46621 taatagagtt gcgatattgc ggtaacgtcc gcgtcaactc gttgagcgcg gaacccaagt
    46681 aagctaatag caacaccatc gccatcacga tgatggtcat caaaatcgac actgccagta
    46741 cacgcggcac acgccagcgg accatgtgtt gcaccagcgg gttaagaata acagcaataa
    46801 ataatgcgag aataaacggc acgatgatct cggcggcaaa acggataccg cagagaataa
    46861 tgaccagcat tcccaacata atgacgattt ttaggccatt gagcgtgatg atcggctttg
    46921 ccatgcttgt ccccgttttt tcttcctttg ctaataataa tgtctttttc gctccgtcat
    46981 cataacgaaa atcaaaccgc tcaggtaaag aaatgaaaat attttgagtt aattcttaag
    47041 ctatgataca aatcaggcgt gttcaactac cgaggacaat tatcatccgc gatgacgaga
    47101 agcaacactg cggataattg taatattatg gacaatttgt tcaggacgtt attttctaca
    47161 tttacccatc ttcgtacctc atccactatc ttgctcgtag gtgagcagca ctggcgcaac
    47221 gcgctttagt cctttcttct gccaaagctt gcgctaagcg cggggcttga ccactttact
    47281 ttggtttcgc tgaattaagc gaaaattaaa ataattctct tgcaggagaa ggacaatgta
    47341 tatttattgg attttattag gtctggctat tgctacagaa attaccggta cgctgtccat
    47401 gaaatgggcg agcgtcagtg agggaaatgg cggctttatt ttaatgctgg tgatgatttc
    47461 tctgtcgtat atatttctct ctttcgccgt taaaaaaatc gccttaggcg tagcttatgc
    47521 gctgtgggaa ggtatcggta ttttatttat taccttgttt agcgttttgt tattcgacga
    47581 aagtttatcg ctgatgaaaa ttgccgggtt aaccaccctg gtcgccggga ttgtgttgat
    47641 aaaatcaggt acccgtaaag cgcgtaaacc tgaactggag gtgaaccatg gcgcagtttg
    47701 aatgggttca cgccgcctgg ctggcattgg caatcgtgct ggaaatcgtt gctaacgtct
    47761 ttttgaaatt ttctgacggc tttcgtcgca aaatatttgg cttgctctcc cttgcggcgg
    47821 tgctggctgc ctttagtgcg ctttctcagg ccgttaaagg gatcgacttg tctgtcgctt
    47881 atgcattgtg gggcgggttt ggtattgccg ccacgttagc cgcaggttgg atcttgtttg
    47941 gtcaacggtt aaatcgtaaa ggctggattg gcctggtctt gctgttggct ggaatgatca
    48001 tggtgaaact tgcctgatga agacgctgcc cgcgctgtcg ggcagcgttt gaacattatt
    48061 tttgcgacag ttgatccagc ttgtcgcgaa aaccggtaac agaaatggcc cggttatcgg
    48121 cgcgccagcg atctttcgcg gcaggagccg aactttgcac cccaattaat tgccagccgt
    48181 catcggtatg caacatcaga ggcgaaccgc tgtcacccgg caaggtatcg cactgatgtg
    48241 acatcaccga cgtttgcgcc cagccagtca cttcacagtt ttgatgactg tacaacgtat
    48301 cgagatgatc ttcagggtat cctgcctgag tcactttacg acctgccgct tttaatgcgg
    48361 cggtaagcgc ggctttatct ccctcaaata acggcaacgg cgtaatgcca gaagggggat
    48421 tacgtagcac aatcaatccg aagtcccacg gcgcggctgc gggaggtaca atccaaccat
    48481 ccccatctgc ttttaaccgc tttcccagtg tcggatcaac gcggccttct atgtcgtgga
    48541 tctcatagcg ccaaagacct ttatttgaca caaaacgcag cgccaccgct ttatcggctt
    48601 taccctttgg aggtgtcaat aaacagtgtc ctgccgttaa tgccagattg ggtgcaatca
    48661 gcgtcgccgt acataaattg ccgctggccg tttccagttg cccaaccgca tcccacggtg
    48721 attgggtcgt gtcattcact ggcacacgat catcatgacc aaaaaacaag gtgctgacct
    48781 catcgtttgc cgatttggca acgtctggtt tatctgcaaa cacaaaagca gacgtcaaac
    48841 taattgcacc caacactaca gcaatggttg tacgcatatc acactctggt gggtaattat
    48901 gattattaaa agcaaaccct cataaatact atagacggga cgggatgaaa gtgggagtaa
    48961 aatcagataa ctacagtcag gaaagataaa aacgggcggc aatcagtgcg cataaaataa
    49021 ggatgataag aatgaactca aagcgataac ggcccaccat actaccctcc gctaaaggcg
    49081 gtgctaaacg ttacccgctt agcaccgtaa ttttcaggcg cgagggggcg cgccagcatt
    49141 actgttgaaa acttacgctg cgggttgtgc agcaggtttt gccggttgct gatggctgtg
    49201 tttcttggcg tgtttcttcg ctgcctgcgc tttttgttca ggggctttct gttcagcttt
    49261 cgcattttta tgatgctttt tagccgcctg cgctttctgg gcaggtgctg ctttatgctg
    49321 ttttttatga tgtgtagttt tcgccggcgc tgctttggtg gtcgtcgcag tcggagcagg
    49381 tgtggtcgca gtctctgcag caaaggcggc agaagacaga cccatagcag cggcaacaac
    49441 cagagctaat acttttttca ttgtcatacc ctcaatttgt tttttcattt aaccccactg
    49501 cggggccgtt gaaataacta tatccctggg aatggcagac ttccgtgagt ggttggtttc
    49561 agcgtgtaac gatatgtaca aacgctgaat aaattacagc gttgataata acgtattgtg
    49621 acttaaggga aatttagcta ccaataatag tagtcttgat cgggttaact attttactac
    49681 ttacaaagcg gtagaataac tgcgcatcag taataaatga cacacagcaa aatgaatccg
    49741 tttatttggg tacttataat cctgatgacg ctagacgcgc tgcgggaatt ggctggcgct
    49801 tcgtctattt taggatggct attaacgttg gtttgagcag gcaataagtc cggacgggta
    49861 tttaccgcag tccggactta tttttcaggc gtgcagacga cgatgcaaac gcgtcccgac
    49921 cagcagagca atgaccagca tcagagcaat aaatgcgccg acgccgttcc agccatagtt
    49981 atgccagaaa acaccaccca gcgtcccggc aatactcgac cccagatagt aactgaacag
    50041 atacagcgag gaggcctggc ctttagcgcg ttttgcgcgg gggccgatcc agctgctggc
    50101 tactgagtgg gctgcgaaga atcctgctga gaagagtaac attccggcaa agatcagcca
    50161 cagcgagctg aataaggtca tcagtaaacc aaacagcata acccccgtcg aaaacaacat
    50221 caccggaccg cgcccatagc gggtggtcat ggttccggct ttgggtgagc tccatgtacc
    50281 ggtcaaataa gccagcgata ataagccaac cacggcctga ctgacatgcc agggggagag
    50341 catcaaccga tagccgatgt aattaaacag cgtgacgaac gaccccatca gcaaaaagcc
    50401 ttctgcgaac aataacggta atccccggtc acgccagtgc aaacgaaagt tgataaacaa
    50461 cgacttaggt cgcagcgagg tcgggcgaaa atggcgtgat tccgggagga ttttccagaa
    50521 catcaacgcc gaggctagcg cgaaacaacc gatagccgcc agagcaattc gccagttgaa
    50581 aaagtccgtg aagacaccac taattaagcg tccgctcatg ccgccaattg agttgccgct
    50641 gatatacaac cccatcgaaa aggccacgaa actggggtgg atttcctcgc taagataagt
    50701 catgccaaca gctgccacgc cacttaacga aagcccaatc aaggcgcgca taatcaaaat
    50761 gccgtgccag ctggtcatca ttgtcgaaag taacgtacaa atggaggcca acagtagcgc
    50821 cgtgaccatc actggtttgc gaccaatggc atcggatagg gggccagtaa acagcaaacc
    50881 aatagccaac atcgccgtgg aaatggacag tgaaatacta ctgttcgcgg gcgttaagcc
    50941 aaactcctgc gaaagcaccg gaaggatagg ctgcacacaa tagagaagtg caaatgttgc
    51001 cagtccggca gagaacagcg ccagggtgac gcgcataaat tgcggcgtac cgcgtttaat
    51061 aaattgattt ggctgagaaa tgctttgctt gtcagtgtcg cttgccggag cgccatcaac
    51121 agttgtagta cggctcactt gaaatccttg ctaaatatgc ctgtggatca ggcttataca
    51181 taggttagga aaatcgaatt gttctgtcta atatattaat aatctcaaat aagatgtttt
    51241 aaatatgaat attgaacttc gtcatctgcg ttactttgtt gctgttgcgg aagagctgca
    51301 tttcgggcgc gccgctgccc gcctgaatat ttcgcaaccg ccgctaagtc agcagattca
    51361 ggcgctggag caacaaattg gtgcccgact gctggcacga accaatcgca gtgtattgct
    51421 gacggcagca ggaaaacagt ttcttgcaga tagtcggcaa atcctgtcta tggtggatga
    51481 cgctgccgct cgcgctgaaa gactgcatca gggtgaagcg ggggagttgc gcattggttt
    51541 tacttcgtcg gctcctttta ttcgggcggt gtccgatacg ttatcgctgt ttcgccgaga
    51601 ttatcccgat gtccatttac aaacccgcga aatgaacact cgcgagcaaa tcgctccgct
    51661 cattgaagga acgctggata tgggattgct gcgtaacaca gcgttaccgg agacgcttga
    51721 acacgcagtc atcgtccatg aaccgcttat ggcgatgatc ccgcacgatc atcccctggc
    51781 aaataacccg aatgtaacgc tggctgaact ggcgaaagaa ccctttgtct tttttgatcc
    51841 gcacgtcggg acagggctgt atgacgatat tctcgggctg atgcgacgtt accatttgac
    51901 gcccgtcatc actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg
    51961 tctgggtgtt tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg
    52021 ggtgccgatt gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca
    52081 tgaacaaagt ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg
    52141 gaaatttcag cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt
    52201 gacgacacgt attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat
    52261 agggagtatg cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc
    52321 aacgcgggcg cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt
    52381 tcccgtctgg cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa
    52441 gcacacctgg tgcaagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg
    52501 gggctggtgg ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag
    52561 attttccttg ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaactg
    52621 gcgttaaaag atgactcgcc attgctggat cgtatcattt cccatatcga tcagtttttt
    52681 atccgccacc agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt
    52741 attgatacgg aaaatggcat tgtacatcgc atgccgttct acgaggatgt aaaagagatg
    52801 ccgctcggag aggctctgga gcagcatacc ggcgttccgg tttatattca gcatgatatc
    52861 agcgcatgga cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt
    52921 caggtggtta tcgatcacaa cgtgggggcg ggcgtcatta ccgatggtca tctgctacac
    52981 gcaggcagca gtagcctcgt ggaaataggt cacacacagg tagacccata tggtaaacgc
    53041 tgttattgcg ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag
    53101 ctggcacagc tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc
    53161 gtggactcat tgtgtcaggc ggcattgcgc ggcgatctgc tggcaaaaga catcattacc
    53221 ggggtgggcg cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa
    53281 aaaatactga ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca
    53341 gatagcatcc gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact
    53401 cagttttcta accagggcac gatggcaggg gctgcgctgg taaaagacgc gatgtataac
    53461 ggttctttgt tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt
    53521 gcgctatctc aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc
    53581 tggtttatat ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac
    53641 cggtacagac acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc
    53701 ctcccaggga aaaacggttg cgggatataa acccgtagcg aaggggagca aagagacacc
    53761 cgaagggctg cgtaataaag atgccctggt gttgcagagt gtttcaacca tcgaactgcc
    53821 ttatgaagca gttaatccta tcgcgttaag cgaagaagaa agtagcgtag cgcacagttg
    53881 cccaatcaat tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca
    53941 tgtcgtggta gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc
    54001 tgaatgggtg gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg
    54061 cattaaccat gcactgctaa cagctcaggc gattgccaac gacgggctgc cgctcattgg
    54121 ctgggtggct aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct
    54181 cggtaaaaaa cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca
    54241 gcgtgaactg gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag
    54301 agtcacggtg taacgtccgc gagattaccg acgcaattac gcaggcaatc aataaaccgg
    54361 ggagtagctg atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt
    54421 gcgtggttgc cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt
    54481 cgccatcagg gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga
    54541 taaatagcgt cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg
    54601 cacacagttt acaaaggaag atcccggcaa tgatcattaa cagtggcggg gcggttaaaa
    54661 aggattgtac ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac
    54721 ccacgatcaa cccgcctagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc
    54781 cacgatgaca ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac
    54841 ctgtactgat aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgctgt
    54901 agagtaacgc gtcgctatga ttaatcagat tgctaaccag caatgccacg acggcggaaa
    54961 taatcaccgg gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata
    55021 aactgccagc aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga
    55081 tccataattt ccactcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg
    55141 cagctaaaag aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca
    55201 gagaggcaag cgatttaacc aggcttgctg cgtaatcaaa ctgaccatcg gtttgcaacg
    55261 cttccatata atcggtcggc gcatgagggc gttgctgggt aaatttctgc cagcccatca
    55321 gcaacaaacc cgccgccagt ccgccgagcg ccggagttag cagccgtcgc cagggggaaa
    55381 ggtttgtcgc tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta
    55441 gcagcatcgc atgacgaaac ccggcaacgg caaaggccgc gagaataccg acgactgttg
    55501 cgataagcag acggcggaac atagtgcgca gattcggata gatgtgtaga tgatgcatga
    55561 gcagaaatgg gcaatgaaac acgggcgact attttgcggc aaaagaggcc agccgcaaaa
    55621 gcagtgcgtg ctttaagaga taaaaaaaaa cgtgacactg taacgtatta ttgccgaatt
    55681 gcgcgtaatc gtcaccatcc ggcaatatta tggtgatcct atcgaaacag cggtttaacc
    55741 gcgacaggaa ttaacagttg tgattgccat tgcgccagcg ttaagcgagc cagtttaccc
    55801 agtgcacggt agaaagggtg ttctgctttt tcgataaaaa cgtcaagaaa acgtgttgac
    55861 cacggaaaaa ggtgccatgc cagcaattct tcgcattctg tctggcgacc attctccgcc
    55921 aaccacgcag ccatcaacag cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt
    55981 tcaaactgaa tgcctttctc gcgcatccac tgacgaagtg ccaatgttga atcgccaaac
    56041 agcacagatt cgcgatccag ccagaccgac ccccacggcg gtgaaggcag tgcccacggg
    56101 ccgacaaaca aacgctgcca ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc
    56161 gtcaccagcg gcgctaatga cgcttctggt agaggccact gagtttccca gccgtcacgg
    56221 gtgagtaccg caaccagagg tgcggcttcc gcactctctg gagcgtaata aaacagcgcg
    56281 cccaacacgc gcgccacgac agaaaaatta tcttgctgtg aaaaatgggt cattcctaac
    56341 atcctgaaat gtgcgggttg ccccgcacca atttgttaac ctgcaatagc cattcctacc
    56401 gtcatgtgca ggccataaaa gagcacacgc ccgatcatct caccgccaag aatgaggatt
    56461 aaccccagaa taagtccggc aacgtgaggt tcacggcgac gaatgagcgg acaaagccag
    56521 catcctagac ctgcgcataa caataccaca cgccagacct gtaaggaggc gtaatctggt
    56581 accagtgcgc tggcttgttg cacggatgag tgaatagacg acagagacaa accttgcagc
    56641 acaatcaccc cggcacaagc gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta
    56701 ttaaatgtaa cgcgggctgc ccgcagaatt gcggcagcaa gtattggacc actcaacaat
    56761 accgtcagga agaaagccag cgtagtgtaa ccgttatgcc aggttggcac agtgtcgatt
    56821 tgatacacgc aggtcatcat ccagacgaaa atgacgccga gcgccatgct gacaagtaac
    56881 cagagtttcc ccaacgcttg cggcatttta ccgatgaccg ccaccagcca ccatagtccg
    56941 ccaacggcaa aaaatatgga accggcagca atctcgttac ttagaccaga agctccgatt
    57001 cgattcagcg agttaaacgc ccgcagtggc gatcccaggt gcatgacaga ggcaataaac
    57061 ccgatgccca tcaacagcca gaggaaaaac atgccacgaa caatgtgctg acggtctgcg
    57121 tcatttttgg cggcgaacca gccaattccg ctaacaatca gcgcgcctac tacgcattga
    57181 cccaatacgg taaagataac cagtggccat tcatgccatc catttcccat tttacacttc
    57241 ctccggatta gccagataac cagtggtatc gccggtcggg cggctgttgg cgttaggttt
    57301 gataacgata ttgggttttg tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt
    57361 gccgtgcttc tggcgcagct cttcaattgg accgaactca agtgcacgca gcgggcagga
    57421 ttcgacacat atgggttgtt tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt
    57481 catgtgccct ttttcagcat tgtactgtgg cgcgccgtac ggacaagcca tgtggcagta
    57541 gcggcagccg atacaaacat cttcatcaac caccacaaag ccatcttcac gcttatgcat
    57601 tgcgccgcta gggcagactt ttgtacatgc ggggtcatcg caatggttac aggagatgga
    57661 gagataataa gcgaacacgt tctggtgcca gacgccatta tcttcctgcc agtcgccgcc
    57721 agcatattca taaatacggc ggaaactgac ttccgggcca agatctttaa aatctttgca
    57781 cgccagttcg caggttttac agccagtaca gcggctggaa tcgataaaaa atccatattg
    57841 tgtggtcatg ggctactcct taaacctttt cgatctggac aagattgctg tgcgacgggt
    57901 ttccctttgc cagcggtgaa gggcggtgag aggtcagaat attaatactg ccgccgtgat
    57961 cgacccggtc accaaacata tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc
    58021 caggcagaat acgcggagtc acttttgcgg caatcagcat ttctccatta ttgttaaata
    58081 cccgcacggt atcgccatga cggataccgc gagcctgagc atcaatgggg ttgatccaca
    58141 cctcttgtgg gcaggcctgc tgtaacacat caatattgcc gtagctggag tgggtacgcg
    58201 ctttgtagtg gaagcccgtt aactgcagtg gataggtttt ccgcagggga tcgtcccagc
    58261 catcaaaacc tggggtatac gcaggaaggg gatgaataat ttcatctttt ttcaattccc
    58321 aggtatctgc aatcttcgcc agtcgttcag aataaatttc gattttcccc gaaggtgttt
    58381 tcaacgggtt tgcctgtgga tcttcacgga atgcgcggaa agcgacgtag tgttcttccg
    58441 ggcatttttt cttaaagatc ccggtcgttt tcatctcctc gtagtcgggc atcttagggt
    58501 tacgttcctt cgttttcgca tggagatatt tgatccattc atgctgactg cgaccttcag
    58561 taaaggtttg ataaacgtcc ggtcctaagc gtttggcgac ttcactcagc atccagtaga
    58621 tgggtttgcg ttcaaatttt gctgaggttg cgggttgggc gaggatcaca tagcccatat
    58681 tccctgcaga ttcatgagag ataaggtctt cttgctctgt tggcatcagg tcgggcaaca
    58741 ggatatcgca atacttagcc gaggccgtca tgaagtggtc aatgccaaca atcatctcgc
    58801 acttgctgtc atcctgaagc acctcatggg tgtgattgat gtcgccatgt tgattgatca
    58861 atgtgttact ggcgtagcac cataaaaact tgatggggac atccagtttt tcttttccac
    58921 gaacaccatc acgggtcgcg gtcatttccg taccatggtc gatggcatct gtccatgtaa
    58981 agacggaaat ctgcgtttta acaggattct cgagcatcgg gaaccattct acccccagat
    59041 cccagctacc ttcgcgtacg cctgagttgc cgccgtttat gccgacgtta ccggtgagaa
    59101 cggaaagcat ggcaatagcg cgggatgttt gttcgccgtt ggagtgtcgt tgtggccccc
    59161 aaccctgaca aatataagca ggttttgctg aaccgatctc tcgtgccaac tggataattt
    59221 tttctgccgg gatgctggtg atttttgctg cccattccgg cgttttagct atgccgtcag
    59281 gcccttcgcc cagaatatag gctttataat gcgcgttacg tggtgcgttg gcgggcagcg
    59341 ttttttcatc gtaaccaaca caatatttgt cgagaaatgg ctgatcgacc atgttttcag
    59401 taatcagtac ccaggcaatc gcacaggcca gtgcgccatc ggtgccaggg cgaatgggca
    59461 gccattcatc ttcacgcccg gcagcagtgt cgttataacg tggatcgatg acgatcatgc
    59521 gtgcgtttga acgttcgcgg gcttgctcga cgtagtaagt gacaccaccg ccgctcatcc
    59581 gcgtttctgc tgggttattt ccgaacataa cgaccagttt cgtattggcg atatcatccg
    59641 ggctgttgcc atcattggca ccgaacatat aactcattgc ggcactgatc tgtgcggtac
    59701 tgtagctacc atagcgactg agaaagccac cgcaagagtt catcagacgg tacgggacgt
    59761 ttgagttggt gatgtttccg ccatctacac ctgttccgta caggacatgt acagcctcat
    59821 tgccgtaatc tttcaggatc cgccgaagat tatcactgat ggtatccagg gcttcgtccc
    59881 aacttatccg ttcaaattta ccttcaccgc gcttgccgac gcgcttcatg ggatatttca
    59941 acctatcagg atgattcatc cgtcggcgga tagagcgccc gcgtaaacac gctcgaacct
    60001 gatgattacc gtagacgtcg tcacctgtcg tatcagactc cacccagtac acggtgtcat
    60061 ctttcacatg caaacgtaac agacagcggc tcccgcagtt aacggtgcag gaactccaga
    60121 ccgctttctc ttctaccgga gcctctgccg cccggaccat ttgggaaaat ggcagagtga
    60181 aagcactgct tgccagcgca agactgccaa gtgcggaggt tttcatcaga cttctacggc
    60241 tgatttcagc cttcatgagc gcctctgtgg tatggatttt catcattact cacttattgc
    60301 ttttcaaaca aaatgtcatg ccagaattta tggttgtagt gggttatatt ttttcgatct
    60361 cgaccagatt cgtatgctgc gggtttccct tcgccagtgg tgaagggcgc agagtggtta
    60421 gcgtattcac acagcctcca tggtcgattt tatcgccaga catattggcc tcgtgccagg
    60481 ctccctggcc catagcgcta accccaggga gaatgcgtgg tgtcactttg gctggtagcc
    60541 gaacttcgcc acgatggtta aacacccgca ccatatcgcc gttggcaatc ccacgtttct
    60601 gcgcatctat agggttgatc cacacctcct gacggcaggc agccttcagg agatcaatat
    60661 tgccgtaggt cgagtgagta cgggatttgt aatggaaacc aaacagttgc agtgggaagg
    60721 ttctacgttc aggtgagtcc cagccttcaa aggtagaggc ataaactggc agtgggctta
    60781 tcacttcatc tttttccagt tcccaggtac gggcaatttc cgccagcttg ctggaataaa
    60841 tttcaatctt accggaaggt gttttaagtg gatttgcctc ggggtcgtca cgaaatgctt
    60901 tgtaggcgac aaaatgacca ttgggatctt tacgcttata gatacccatt tttttcagtt
    60961 cgtcgtaaga cggtaacgcc ggatctttgg caagcatttt ggcgtacaga tgttgtaacc
    61021 attgttcctg cgtgcgacct tctgtgaact tttgatagac gtcaggtcca agacgtttcg
    61081 cgacttcact caggatccag taaatcggtt tgcgttcgaa tttttcgctg gtgacaggct
    61141 ggaggaaaat gagatatccc atgttaccgg cgtagtcgtt aggaataata tcttcctgct
    61201 caacggtcat caggtctggc agcagaatgt cggcatattt tgccgatgag gtcataaagt
    61261 tttcgatgac cacaatcatt tcgcatttcg attcgtcttg cagaatttca tgcgttttgt
    61321 tgatgtcaga atgctgatta accagggtat ttcccgcgta gttccagatg aacttaatgg
    61381 gcacatccag tttatctttg ccgcggacgc cgtcgcggat tgccgtcatt tgcgggccat
    61441 gatcgatagc atccgtccag ctgaagcagg agattgacgt tttgaccgga ttatccagca
    61501 ccggcaggcg ctctatggta atggtatagg tcgattcacg cgcgccacta tttccgccgc
    61561 tgatgccgac attgcccgtc aaaataggca acatagcaat agcgcgtgca gtcagctcgc
    61621 cgtttgcctg gcgttgcggc ccccagccct ggcagatata agcgggtttt gctgtgccaa
    61681 tttcacgcgc cagtttgatg atacggtcca ccgggatacc ggtaatttgc gaagcccact
    61741 gcggcgtttt cgctgtgtta tcatcacctt caccaagaat ataggcttta tagtgaccat
    61801 tttttggtgc atctgcgggt aaggtttttt cgtcatagcc gacgcagtat ttatcgagaa
    61861 aaggttgatc aacgagattt tcgttaatca atacccaggc aatccccgca accagcgcgg
    61921 catcggtgcc cgggcgaata ggaagccatt catcctcacg accagcagcc gtatcggtat
    61981 atcgcggatc gataacaatc attttggcgt tcgatttctc gcgcgctttt tcaagaagat
    62041 aagtgatgcc accgccgctc atgcgggttt ctgccgggtt gttaccaaac atcacgacca
    62101 gcttgctgtt ttcaatatcc gtggtgctgt tgccatcatt actgccgtag gtgtagggca
    62161 tggcacagga aatttgtgcg gtgctgtagg agccatactg attgagtgaa ccgccgtagc
    62221 agttcatcag gcgtttgacc gccgaggctg atggcgaaga gcgggtcata ttgccgccaa
    62281 cgatccccga agagtactga atatatacag cctcattgcc atattgttcg acggttttct
    62341 tcaggctact ggcgatagta tccagggctt catcccagct aatccgttcg aatttgcctt
    62401 caccgcgttt acccacgcgt ttcattgggt aattcaagcg atcgggatga ttaatacgcc
    62461 ggcggatgga gcgaccgcgc aaacaggcgc gtacctgatg gttgccatac tcatcgctgc
    62521 cggtgttgtc agtttccacc caggccactt cattatcttt aacatgtaga cgaagtgcac
    62581 agcggctacc acagttgacg gaacaggcac cccagaccac ttgttcgctg gcctgttgta
    62641 ctgctgccgc tgcactgcgc agggtgaacg gcaaagaaaa accgcctgca gccagcgcca
    62701 gagaacctat cgcggtagat ttaacgagtg ttctgcggct gatgcccacc atttgttcat
    62761 ttttggacat aactcactcc ctgttcttta tcgttatata aaagtttata tattgaatat
    62821 ttagcgcgct aacaatagag ggagtctacc cattttgggt taagaattat taatccatat
    62881 caatagaagg gtatgagtaa taaggtgggt tatgttatat gttcaaatcg ccggatgcgt
    62941 cgtatccggc gttcagtcga taatgtatta ctgcggttcg gcaggcgcgc catcctgggt
    63001 agactgcgcg ggagcagaga cgttaccgct ggtggtgcgg gtgtagagaa ttttatgcgt
    63061 atcattagcg caatgcccga cgacctggga atcaggctga tcaacctggt cattgggtac
    63121 aatacttaac gtgaagctgc tttcgggtac gccattattg ataatgcgct gtgatatatc
    63181 gctttgtatg cgctcacagg atcccggcgc ggcgagtacc gcgggtgagg cgagggcgag
    63241 cagaagcgcg gcacagcagg ttgagagttt catcataagc tccttacgcg aagataactt
    63301 ctttaagcat agcatttaac gtgtaaagta ctgtatttgc tactatgatt gagaatcatc
    63361 tctactctct ggtgactgat gtgaaataca aattactgcc atgcttactc gcgatattac
    63421 tcacaggatg tgaccgcaca gaggtaacac tttcatttac tcctgagatg gccagtttct
    63481 ctaatgaatt cgattttgat ccgctgcgtg gtccggtaaa agatttcact cagacattaa
    63541 tggatgagca aggtgaagtg acgaaacgtg tttcggggac tttgtcggaa gaaggctgtt
    63601 ttgattcact cgaattactg gatctggaaa ataataccgt ggtcgctctg gtactggacg
    63661 ccaattatta ccgtgatgcc gagacgctgg agaagagagt acgtttacag ggaaaatgcc
    63721 agctagcaga attaccttct gccggggtga gttgggaaac cgatgataat ggcttcgtga
    63781 ttaaagccag cagcaaacaa atgcagatgg aatatcgcta tgatgatcag ggttatccac
    63841 tgggtaaaac cacgaaaagt aacgacaaaa cattatctgt cagcgccacg ccatcaacgg
    63901 acccgatcaa aaaattagat tacacagcgg ttactttact gaataatcaa cgagttggta
    63961 atgtaaaaca gagctgtgaa tatgacagtc acgccaatcc ggtggactgt cagctgatca
    64021 ttgttgatga aggagtaaaa cccgccgtcg aacgggttta caccatcaaa aatacgatcg
    64081 attattatta atgctattgt gcggtcggct tcaggagagt ctgacccggt gttttgtgct
    64141 ctgccagata ctgatgctgg aatatacaca tgcgaatggc attacgatat tgaccattaa
    64201 taaagaactc atgcatcaat tcaccttcaa ccgtaaagcc aagcttgcgg taaatgtgaa
    64261 tcgctttttc attctcttta tcaacgatca gatacagctt atagagattg agaacggtga
    64321 agccatagtc cattgctaat ttggcggcac gggttgccag acctttcccc tgatactccg
    64381 gggagataat tatctgaaat tctgcgcggc gatgtacatg gttaatttcc accaactcca
    64441 ccagaccggc tttttcgccg tcacattcca ccacaaagcg tcgttcgctc tgatcgtgaa
    64501 tatgcttatc atacagatca gagagttcaa caaaggcttc gtagggttcc tcaaaccagt
    64561 aacgcatcac actggcgtta ttgtcgagtt gatgtacata gcgtaaatct tcacgctcca
    64621 gcgggcgtag cttaacactg tgggcgcttg gcataacgtg tccttacatt ccttaaatca
    64681 ataacaggtt agggggtaat aacgcggcca gttcgacggt ccaggcagcg caaagtattt
    64741 ggctcccagt aggcattgat gttggcgctt tgctcacatt tatcgcggtt atcaaaagcg
    64801 gcgtcggctt tatcccactc tttttcagtg cgtttattca ctttctgacg cagattgcgc
    64861 gtgtcattcc attgctcttt ttccatagcg gcgtgctggc ggctttgtgc actgtcgcca
    64921 gactcaatca ccagtttgtt agtttcggca tgaacagttg tgctcaatgc cagtgtgcaa
    64981 ggcagcagaa aagcgagcag gccgattcgt ttgctgagag tgattttcat aattcattcc
    65041 ctgtatgaat gattaaaggt gattctacac catccactgc gaacgcaaaa cgtaccagga
    65101 gggtgtttat attgatgata ttatgtcgcc ctataactat acatgatgtc aataagtgac
    65161 aaagatgatt aaaacaacgt tactattttt tgctactgcg ctgtgtgaaa ttattggatg
    65221 ctttctgccc tggttgtggt taaaacgaaa cgccagtatc tggctgttgc ttccggcggg
    65281 gatttcactg gcgctgtttg tctggttgtt aacgttgcat cctgcggcga gtgggcgtgt
    65341 ttacgcggct tatggtggcg tttatgtctg cacggcgttg atgtggctgc gcgttgtgga
    65401 tggcgtgaaa ctgactcttt atgactggac gggtgcgttg attgcgcttt gcggcatgtt
    65461 gatcattgtt gccggctggg ggcgcacgta aggacataaa tctattttat caataagata
    65521 cgaggaagcg tcagctgata aaaggtactc tatttcatct tttgtcaacc attcatggcg
    65581 aaaatatacg cctttttttg tgatcactcc ggcttttttt gatcttcata cttgtatggt
    65641 agtagctcag ttgcgtagat ttcatgcatc acgacaagcg atgcaaggaa tcgaacatga
    65701 agatcgtaaa ggctgaagtt tttgttacct gtccggggcg taatttcgtc acattaaaaa
    65761 tcaccactga ggacggtatt acgggccttg gggatgccac cctcaatgga cgtgagcttt
    65821 ctgtggcctc ttatttgcag gatcaccttt gtccgcagct tattggtcgc gatgcgcacc
    65881 gtatcgaaga tatctggcag tttttctata aaggtgctta ctggcgtcgc ggtccggtta
    65941 cgatgtcggc catttcagcg gttgatatgg cgctgtggga tattaaagcc aaagctgcca
    66001 acatgccgct ttaccagtta ctcggcggcg cgtctcgtga aggggtgatg gtttattgcc
    66061 ataccaccgg tcacagtatt gatgaagctc tggatgatta tgcccgtcat caggagctgg
    66121 gattcaaagc catccgcgtg cagtgcggca tccctggtat gaaaaccacc tacggcatgt
    66181 cgaaaggtaa aggtctggct tatgaacccg caaccaaagg acagtggccg gaagagcagc
    66241 tgtggtcgac ggagaaatac ctcgatttca tgccgaaatt gtttgacgcg gtacgtaaca
    66301 agtttggttt taatgaacat ttgctgcatg acatgcacca tcgcttaacg cctattgaag
    66361 cggcgcgctt tggtaaaagc attgaagatt atcgcatgtt ctggatggaa gacccgacgc
    66421 ctgcggaaaa ccaggaatgc ttccgtctca ttcgccaaca taccatcaca cccatcgcgg
    66481 tgggtgaagt cttcaacagc atctgggact gcaagcaact gattgaagag caacttatcg
    66541 attatatccg caccacgctg acccatgcag gcggaattac cggtatgcgc cggattgccg
    66601 attttgcttc gttgtatcag gtacgtactg gctcacacgg tccttccgat ttgtcaccag
    66661 tctgcatggc tgcggcgctg cactttgatc tgtgggtccc caatttcggt gtccaggaat
    66721 acatgggtta ttccgaacaa atgctcgaag tcttcccgca caactggact ttcgataacg
    66781 gctatatgca tccgggagac aaaccgggtc ttggcatcga attcgatgaa aagctggcgg
    66841 cgaaatatcc ctatgaacct gcttatctac cagtcgcacg tctggaagat ggcacgctgt
    66901 ggaactggta aggagtaagt taatgaaaag cattttaatt gaaaaaccga atcaactggc
    66961 gattatcgaa cgtgaaatac ccaccccgtc agcgggtgaa gttcgagtaa aagtaaaact
    67021 tgccggaatt tgtggttcag atagccatat ttatcgtggg cataatcctt ttgcgaaata
    67081 tccgcgcgtc attggacatg aattctttgg cgtcattgat gcggtgggtg aaggtgtgga
    67141 aagcgccaga gtcggtgaac gtgttgctgt cgatccggtg gtcagctgtg gtcattgcta
    67201 tccgtgctct ataggtaagc cgaacgtttg tacgacactc gctgtattag gtgtgcatgt
    67261 tgacggtggt ttcagtgaat atgctgtggt tccggcaaaa aatgcgtgga aaattcctga
    67321 agcagtggcc gatcaatatg cggtgatgat cgaacctttt accattgcgg ctaacgtaac
    67381 cggacatggt caaccgactg aaaatgatac cgttctggtt tatggtgccg gtccaatcgg
    67441 cctgacgatc gttcaggtat taaaaggcgt ctataacgtt aaaaatgtga ttgttgccga
    67501 tcgcattgat gaacgactgg aaaaagcgaa agagagcggg gctgattggg tgattaataa
    67561 cagccagaca ccgcttggcg agattttcgc tgaaaaaggc ataaagccga cattaattat
    67621 cgatgcggct tgtcatcctt ctatcctgaa agaggccgta acgctggctt ctccagcggc
    67681 acgtattgta ttgatgggct tctccagtga accttctgaa gtgattcagc aaggaattac
    67741 cggaaaagaa ctctctattt tctcttcacg cttaaatgca aataaatttc cggtcgttat
    67801 cgactggtta agtaaagggt taattaaacc agaaaaatta attacccata cgtttgattt
    67861 ccagcatgtt gctgatgcca ttagtttatt tgaacaggat caaaaacatt gctgcaaagt
    67921 cttactcact ttttctgaat aataccaata acggcgagta agtagtacgc atcttacctc
    67981 ttttttagag ataaccatta tgacaataga aaaacacgaa agaagcacta aggatttggt
    68041 gaaagcagca gtatcgggat ggctgggcac tgcgcttgaa tttatggatt tccagttata
    68101 ttcgctcggc gcagcgttag tgtttcatga aatatttttt cctgaatcat caacggcaat
    68161 ggcgttaatt ctggcaatgg gaacctacgg tgcaggttat gtggcgcgta ttgtcggagc
    68221 atttattttc ggcaaaatgg gcgacagaat agggcgtaaa aaagtgctct ttattaccat
    68281 caccatgatg gggatctgta ccaccttaat tggagtgtta ccgacctatg cacagattgg
    68341 tgtttttgcc cccatcttgc tggtgacgct gcgtattatt cagggattgg gtgcaggtgc
    68401 ggaaatttcc ggtgccggta cgatgctggc ggaatatgcg ccaaaaggta agcgcggaat
    68461 tatctcctca tttgtggcta tgggaactaa ctgcggaacc ttgagcgcaa cggcaatctg
    68521 ggcctttatg ttcttcattc tcagtaaaga ggaactgctg gcgtggggat ggcgtatacc
    68581 gttcctggcg agcgttgtcg tgatggtctt tgctatctgg ttgcgtatga atctgaaaga
    68641 aagcccggtc tttgagaagg ttaacgacag caaccaaccg acagcaaaac ctgcacctgc
    68701 tggtagcatg ttccagagca aatccttctg gctggcaaca gggctgcgtt ttggtcaggc
    68761 aggtaactcc gggttaattc agactttcct tgcaggctat ttagtacaga cgttattgtt
    68821 taacaaagca attccaacag atgcattgat gatcagttcg attctcggct ttatgaccat
    68881 tccgttcctt ggttggttat ccgataaaat tggtcgccgg atcccgtata ttattatgaa
    68941 tacctctgcg attgtgctgg catggccaat gctttctatc atcgtagata aaagctatgc
    69001 cccgagcacc attatggttg cactgattgt gattcataac tgtgcggtgc tgggattatt
    69061 tgctctggaa aacattacca tggcagaaat gttcggctgt aaaaaccgct ttacccggat
    69121 ggctatttct aaagaaattg gtggtcttat cgcttccggt tttggtccta tccttgcggg
    69181 tattttctgc accatgacgg aatcctggta tccgatcgcc attatgatca tggcatattc
    69241 agtgattggt ttaatctctg cgctgaaaat gccagaagtg aaagaccgtg atttaagtgc
    69301 gctggaagac gctgcggaag atcaaccgcg tgttgtaaga gctgcgcaac cttccagaag
    69361 tctttaaatc ctcaatccct tctcttac
//
LOCUS       DAZGBC010000007        68299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGBC010000007 DAZGBC010000000
VERSION     DAZGBC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.007"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..1051)
                     /gene="dusC"
                     /locus_tag="ACHK1J_000456"
     CDS             complement(101..1051)
                     /gene="dusC"
                     /locus_tag="ACHK1J_000456"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO7909795.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIDKLR"
     gene            complement(1211..2404)
                     /locus_tag="ACHK1J_000457"
     CDS             complement(1211..2404)
                     /locus_tag="ACHK1J_000457"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO7909796.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2419..3063)
                     /gene="maiA"
                     /locus_tag="ACHK1J_000458"
     CDS             complement(2419..3063)
                     /gene="maiA"
                     /locus_tag="ACHK1J_000458"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO7909797.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3072..3773)
                     /locus_tag="ACHK1J_000459"
     CDS             complement(3072..3773)
                     /locus_tag="ACHK1J_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO7909798.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3789..4817)
                     /gene="gtdA"
                     /locus_tag="ACHK1J_000460"
     CDS             complement(3789..4817)
                     /gene="gtdA"
                     /locus_tag="ACHK1J_000460"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO7909799.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4829..6187)
                     /locus_tag="ACHK1J_000461"
     CDS             complement(4829..6187)
                     /locus_tag="ACHK1J_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7909800.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6314..7222
                     /locus_tag="ACHK1J_000462"
     CDS             6314..7222
                     /locus_tag="ACHK1J_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7909801.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7353..7751
                     /gene="yohJ"
                     /locus_tag="ACHK1J_000463"
     CDS             7353..7751
                     /gene="yohJ"
                     /locus_tag="ACHK1J_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO7909802.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7748..8443
                     /gene="yohK"
                     /locus_tag="ACHK1J_000464"
     CDS             7748..8443
                     /gene="yohK"
                     /locus_tag="ACHK1J_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO7909803.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPMILAVMG"
     gene            8573..9457
                     /gene="cdd"
                     /locus_tag="ACHK1J_000465"
     CDS             8573..9457
                     /gene="cdd"
                     /locus_tag="ACHK1J_000465"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO7909804.1"
                     /translation="MHPRFQTAFAQLAENLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            9607..10326
                     /gene="sanA"
                     /locus_tag="ACHK1J_000466"
     CDS             9607..10326
                     /gene="sanA"
                     /locus_tag="ACHK1J_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO7909805.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10329..10568
                     /gene="yeiS"
                     /locus_tag="ACHK1J_000467"
     CDS             10329..10568
                     /gene="yeiS"
                     /locus_tag="ACHK1J_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO7909806.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10762..12000
                     /gene="preT"
                     /locus_tag="ACHK1J_000468"
     CDS             10762..12000
                     /gene="preT"
                     /locus_tag="ACHK1J_000468"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO7909807.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCIAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MVADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            11994..13229
                     /gene="preA"
                     /locus_tag="ACHK1J_000469"
     CDS             11994..13229
                     /gene="preA"
                     /locus_tag="ACHK1J_000469"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO7909808.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(13472..14482)
                     /gene="mglC"
                     /locus_tag="ACHK1J_000470"
     CDS             complement(13472..14482)
                     /gene="mglC"
                     /locus_tag="ACHK1J_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO7909809.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14498..16018)
                     /gene="mglA"
                     /locus_tag="ACHK1J_000471"
     CDS             complement(14498..16018)
                     /gene="mglA"
                     /locus_tag="ACHK1J_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO7909810.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16079..17077)
                     /gene="mglB"
                     /locus_tag="ACHK1J_000472"
     CDS             complement(16079..17077)
                     /gene="mglB"
                     /locus_tag="ACHK1J_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO7909811.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17357..18397)
                     /gene="galS"
                     /locus_tag="ACHK1J_000473"
     CDS             complement(17357..18397)
                     /gene="galS"
                     /locus_tag="ACHK1J_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO7909812.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(18539..19696)
                     /gene="yeiB"
                     /locus_tag="ACHK1J_000474"
     CDS             complement(18539..19696)
                     /gene="yeiB"
                     /locus_tag="ACHK1J_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO7909813.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDTQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFTQFDR
                     LGLLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(19713..20381)
                     /gene="folE"
                     /locus_tag="ACHK1J_000475"
     CDS             complement(19713..20381)
                     /gene="folE"
                     /locus_tag="ACHK1J_000475"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO7909814.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20639..21475
                     /gene="yeiG"
                     /locus_tag="ACHK1J_000476"
     CDS             20639..21475
                     /gene="yeiG"
                     /locus_tag="ACHK1J_000476"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7909815.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21507..23486)
                     /gene="cirA"
                     /locus_tag="ACHK1J_000477"
     CDS             complement(21507..23486)
                     /gene="cirA"
                     /locus_tag="ACHK1J_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO7909816.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(23778..25247)
                     /gene="lysP"
                     /locus_tag="ACHK1J_000478"
     CDS             complement(23778..25247)
                     /gene="lysP"
                     /locus_tag="ACHK1J_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO7909817.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25452..26333)
                     /gene="yieE"
                     /locus_tag="ACHK1J_000479"
     CDS             complement(25452..26333)
                     /gene="yieE"
                     /locus_tag="ACHK1J_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO7909818.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26432..27481
                     /gene="yeiH"
                     /locus_tag="ACHK1J_000480"
     CDS             26432..27481
                     /gene="yeiH"
                     /locus_tag="ACHK1J_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO7909819.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27555..28412
                     /gene="nfo"
                     /locus_tag="ACHK1J_000481"
     CDS             27555..28412
                     /gene="nfo"
                     /locus_tag="ACHK1J_000481"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO7909820.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28415..29503
                     /gene="yeiI"
                     /locus_tag="ACHK1J_000482"
     CDS             28415..29503
                     /gene="yeiI"
                     /locus_tag="ACHK1J_000482"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7909821.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29559..30809)
                     /gene="nupX"
                     /locus_tag="ACHK1J_000483"
     CDS             complement(29559..30809)
                     /gene="nupX"
                     /locus_tag="ACHK1J_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7909822.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     MLSPATESSQVSFNNLSFTETPPKSIIEAAATGAVTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30909..31850)
                     /gene="rihB"
                     /locus_tag="ACHK1J_000484"
     CDS             complement(30909..31850)
                     /gene="rihB"
                     /locus_tag="ACHK1J_000484"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO7909823.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            31980..32678
                     /gene="yeiL"
                     /locus_tag="ACHK1J_000485"
     CDS             31980..32678
                     /gene="yeiL"
                     /locus_tag="ACHK1J_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO7909824.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32746..33996)
                     /locus_tag="ACHK1J_000486"
     CDS             complement(32746..33996)
                     /locus_tag="ACHK1J_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7909825.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFTNASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34090..35028)
                     /gene="psuG"
                     /locus_tag="ACHK1J_000487"
     CDS             complement(34090..35028)
                     /gene="psuG"
                     /locus_tag="ACHK1J_000487"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO7909826.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(35016..35957)
                     /gene="psuK"
                     /locus_tag="ACHK1J_000488"
     CDS             complement(35016..35957)
                     /gene="psuK"
                     /locus_tag="ACHK1J_000488"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO7909827.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQSAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNLIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36381..38072)
                     /gene="fruA"
                     /locus_tag="ACHK1J_000489"
     CDS             complement(36381..38072)
                     /gene="fruA"
                     /locus_tag="ACHK1J_000489"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO7909828.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVASLAYAFLKRPEVDAVAKAA"
     gene            complement(38089..39027)
                     /gene="fruK"
                     /locus_tag="ACHK1J_000490"
     CDS             complement(38089..39027)
                     /gene="fruK"
                     /locus_tag="ACHK1J_000490"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO7909829.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(39027..40157)
                     /gene="fruB"
                     /locus_tag="ACHK1J_000491"
     CDS             complement(39027..40157)
                     /gene="fruB"
                     /locus_tag="ACHK1J_000491"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO7909830.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40525..41706
                     /gene="setB"
                     /locus_tag="ACHK1J_000492"
     CDS             40525..41706
                     /gene="setB"
                     /locus_tag="ACHK1J_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO7909831.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41703..41957)
                     /gene="yeiW"
                     /locus_tag="ACHK1J_000493"
     CDS             complement(41703..41957)
                     /gene="yeiW"
                     /locus_tag="ACHK1J_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7909832.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42112..42684
                     /gene="yeiP"
                     /locus_tag="ACHK1J_000494"
     CDS             42112..42684
                     /gene="yeiP"
                     /locus_tag="ACHK1J_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO7909833.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42907..44373
                     /gene="yeiQ"
                     /locus_tag="ACHK1J_000495"
     CDS             42907..44373
                     /gene="yeiQ"
                     /locus_tag="ACHK1J_000495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO7909834.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            44491..45477
                     /gene="yeiR"
                     /locus_tag="ACHK1J_000496"
     CDS             44491..45477
                     /gene="yeiR"
                     /locus_tag="ACHK1J_000496"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO7909835.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNGLQSALLKLRLATTA"
     gene            45516..46229
                     /gene="lpxT"
                     /locus_tag="ACHK1J_000497"
     CDS             45516..46229
                     /gene="lpxT"
                     /locus_tag="ACHK1J_000497"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO7909836.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFSDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46641..47207
                     /gene="mepS"
                     /locus_tag="ACHK1J_000498"
     CDS             46641..47207
                     /gene="mepS"
                     /locus_tag="ACHK1J_000498"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO7909837.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47388..48944
                     /gene="pdeN"
                     /locus_tag="ACHK1J_000499"
     CDS             47388..48944
                     /gene="pdeN"
                     /locus_tag="ACHK1J_000499"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7909838.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGIEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            49026..50840
                     /gene="yejA"
                     /locus_tag="ACHK1J_000500"
     CDS             49026..50840
                     /gene="yejA"
                     /locus_tag="ACHK1J_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO7909839.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSAKQQGE"
     gene            50841..51935
                     /gene="yejB"
                     /locus_tag="ACHK1J_000501"
     CDS             50841..51935
                     /gene="yejB"
                     /locus_tag="ACHK1J_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO7909840.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPLHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            51935..52960
                     /gene="yejE"
                     /locus_tag="ACHK1J_000502"
     CDS             51935..52960
                     /gene="yejE"
                     /locus_tag="ACHK1J_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7909841.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            52962..54551
                     /gene="yejF"
                     /locus_tag="ACHK1J_000503"
     CDS             52962..54551
                     /gene="yejF"
                     /locus_tag="ACHK1J_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO7909842.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(54555..54899)
                     /gene="yejG"
                     /locus_tag="ACHK1J_000504"
     CDS             complement(54555..54899)
                     /gene="yejG"
                     /locus_tag="ACHK1J_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO7909843.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55232..56422)
                     /gene="bcr"
                     /locus_tag="ACHK1J_000505"
     CDS             complement(55232..56422)
                     /gene="bcr"
                     /locus_tag="ACHK1J_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO7909844.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56450..57145)
                     /gene="rsuA"
                     /locus_tag="ACHK1J_000506"
     CDS             complement(56450..57145)
                     /gene="rsuA"
                     /locus_tag="ACHK1J_000506"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO7909845.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57294..59054
                     /gene="radD"
                     /locus_tag="ACHK1J_000507"
     CDS             57294..59054
                     /gene="radD"
                     /locus_tag="ACHK1J_000507"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO7909846.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAELRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59179..59463
                     /gene="rplY"
                     /locus_tag="ACHK1J_000508"
     CDS             59179..59463
                     /gene="rplY"
                     /locus_tag="ACHK1J_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO7909847.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(59602..60609)
                     /gene="yejK"
                     /locus_tag="ACHK1J_000509"
     CDS             complement(59602..60609)
                     /gene="yejK"
                     /locus_tag="ACHK1J_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO7909848.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60791..61018
                     /gene="yejL"
                     /locus_tag="ACHK1J_000510"
     CDS             60791..61018
                     /gene="yejL"
                     /locus_tag="ACHK1J_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO7909849.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            61038..62798
                     /gene="yejM"
                     /locus_tag="ACHK1J_000511"
     CDS             61038..62798
                     /gene="yejM"
                     /locus_tag="ACHK1J_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO7909850.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFITFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62873..62949
                     /locus_tag="ACHK1J_000512"
     tRNA            62873..62949
                     /locus_tag="ACHK1J_000512"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62907..62909,aa:Pro,seq:ggg)
     gene            complement(63051..65642)
                     /gene="yejO"
                     /locus_tag="ACHK1J_000513"
     CDS             complement(63051..65642)
                     /gene="yejO"
                     /locus_tag="ACHK1J_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO7909851.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKATTRVMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            65962..66609
                     /gene="narP"
                     /locus_tag="ACHK1J_000514"
     CDS             65962..66609
                     /gene="narP"
                     /locus_tag="ACHK1J_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO7909852.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(66644..67696)
                     /gene="ccmH"
                     /locus_tag="ACHK1J_000515"
     CDS             complement(66644..67696)
                     /gene="ccmH"
                     /locus_tag="ACHK1J_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO7909853.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVLEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(67693..68250)
                     /gene="dsbE"
                     /locus_tag="ACHK1J_000516"
     CDS             complement(67693..68250)
                     /gene="dsbE"
                     /locus_tag="ACHK1J_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO7909854.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT        16693 a        18098 c        16822 g        16686 t
ORIGIN      
        1 gcgccagtgg caatatgtgg cttagttcat gaaaggagcg caacaagatg atgaagctga
       61 ataagcgatt cattgtctct gataaactgc gattccacta ttatcgcagc ttatcgatat
      121 caattgcctg aatagccctt gcaatatcat gggaattatt caacacccga acatgctgaa
      181 ataattccgt tgcttcatcg tattctttac gcaaataact caaccactgt ttaatccgcg
      241 ctacgtgata taacccggta tcgccctgct tttccagacg ggtatatttt tgcagcaaag
      301 caaccacctc cggccacggc attcgcggtt cgttatattt taccacccgg ctcaggttgg
      361 gaatattgag cgccccgcga ccaatcatca ctgcgtcgca gccgctgatc gccatgcatt
      421 gttgcgcgct ctgccagtcc cagatttcac cgttggcaat caccggaata ttcagccgct
      481 ggcgaatctc accaatcgcc tgccagtcaa tatgctccgc gcggtaaccc tgctcttttg
      541 tccgtccatg caccaccagc tccgtagcac cagcctgttg aaccgcatcg gcgatttcaa
      601 atttcttctc accgctgtcc cagcccagac gcactttcac gctgacgggc aaatgcgccg
      661 gtacagcttc acgcatcgct tttgcaccct ggtagatgag ttcaggatct ttgagtaacg
      721 tcgccccacc accgctaccg ttgaccgttt tcgacgggca gccgcaattg agatccacgc
      781 cccaggaacc taattccacc gcacgggcgg cgttctctgc cagccattgt gggaactgac
      841 ctaacaactg aacgcgcacc agcgtaccgg atggtgtccg gctggcgttt tgtaactcag
      901 ggcaaatgcg atgaaagact tttaccggca gcagttgatc caccacgcgg acaaactcgg
      961 tgatgcacag atcgtagtcg ttaacttcgg tcagcaattc acgcaccaga gagtcaagca
     1021 caccctccat cggtgccagt aacacacgca tatcatcacc cgcaaaaaaa tgaggcgcta
     1081 tgttagcgcc tcctgtgttt gcagacaaac tgacttactg attaattcgg gcaagatttt
     1141 taccctcacc ctaaccctct cccgaagggg cgaggggacc gcccgtgcgc ctgattgggt
     1201 gaagaagtgc tcagcgtggt tgcaaacagt tatcttcttt ccagccgtac aaccactcca
     1261 tgccgcgata aaattctgcc tgcgatttcc ctttccacag cagattacgt acctggcgtt
     1321 ctacccctgc ggcgtggtaa acccgcccca tttcacgggt cgaccagacg atgcgcgcgg
     1381 tgcgcggaat tcgtaccgac tcatacagcg caaaggcctg cgccgcatca ccctcacact
     1441 gcgccagcgc cttacccagc gttactgcat cttccagcgc catacaggct ccctgcgcca
     1501 tatattgcgc caccggatgg gccgaatcgc ccacaagcgt tatgcgcttc gttccccact
     1561 tcgccaccgg ttcacggtca gcggttgacc agcgtcgcca tgaagtcggt ttatccaaca
     1621 tctggcgcgg acgggggtga atcccttcaa agtaagagag cacttcctct ttactaccat
     1681 ccttcacgcc ccactcttcc tgctggcggc tgtggaatgt taccaccagg ttatactgct
     1741 tgccgccgcg caaagggtaa tgcaccaagt gacaatgcgg ccccgcccac agtaccggtg
     1801 cgttaatgcg caaatcttcc ggcatatcgg cacgatcgat aaccgcacgg tagacaacat
     1861 gtccggtgac acgcggcgca tcgcccaata agctctggcg caccactgac ttcacgccgt
     1921 cacagccaat cagaatatcg ccggtccagc tattgccgcg atcgtcaaac acggtgacat
     1981 cgtcttccgt ctcgcgaata tccacgacgt tggtggaggt gtgatattcc acgtccggat
     2041 gcgtcagtac cgcttcccac accgaggcgt gaatatcaac ccggtggatc accgcgtaag
     2101 ggccgccgaa atggtcacga aaagcctgcc cggtttcaat gcacaccact tcttcggcgt
     2161 tcaccgcatc catcatggta atgtgatcgg taaacaccgc gcgctggcgg gcaacttcgc
     2221 cgacgccgag gctgtccagc gccgaaaacg catttggccc caactggatg cccgcgccaa
     2281 tctcaccgat ctcatgcgcc ttttccagca gcaccacttt gatccccagg cgggccagtg
     2341 acagcgcagt tgccgcaccg ccgatcccgc ctcccacaat tactgcgcgt gtcactttcg
     2401 tcattatagt tctcctggtc aggctgaaat tttatcttgt tgattttctg gcgctgcggc
     2461 aatgaacgct ggcagctgtg tgcaggcgtc gtacaccgct ttgcagcgtg gatacccgct
     2521 caaatcgcag ttcatgcgta gcgcgttcgc ccactgcggg acaaggcagc aatctgccag
     2581 cgtcggcgtc tcgcccacac aaaattgccc cgactggttc tggcgcaaca gttgttccac
     2641 cgcgcttaac ccttgctgga tccagtgcgc ataccaacgt tttttttctt cttcactgac
     2701 gttcagttct tcggtcaggt agcgcagcac gcgcaagtta ttcaatggat gaatatcaca
     2761 agcgatggca tagacaattt ccagcacctt gttgcgggcc ggctcttcct gcggcaccag
     2821 cggaacttgc gggtagtgcc tctccagcca gtcaataatc gccagcgatt gtccgagtga
     2881 ttcgccctcg tcagtcagca gcgtcggcac cagcccgacc ggattcatcc gccgataggc
     2941 cagttcattt tgctgaccaa tacggatatt caccccttcg gtctggtaat taatgccttt
     3001 cagcgccagc gcaatgcgta cccggtacga cgctgaacta ttaaaaaaac tgtacagctt
     3061 catcatgcac ctcagacaat cttcaccgcg ataggcgtta agccctcgac gttgcctgtg
     3121 atcacatcac ctttcactac cgggccaacc ccttccggcg taccggtgaa gatcagatcg
     3181 cccggttgca gctcaaagaa gcctgacaga tagctgatgg tttcattcac cgaccagatg
     3241 agatggcgaa tatcgctgcg ctgatgatct tcgccgttaa cctgtaacca gattggcgca
     3301 ttatcaatac cgccgatatc gcgggctttg tgcagcggcg cgatgggcgc tgagagatca
     3361 aacgctttac cgatttccca cgggcggccc atctggcgca tttccatttg acggtcacgc
     3421 cgagtcatat caagacccgt ggcataaccc cagacatatt catgggcgtg ttccagcggg
     3481 atatcgctgc cttttttacc aatcgccacc accagttcaa tttcgtaatg gtagttatcg
     3541 gtctgtgccg gatacggcag cgccagcgtt tcaccagcgg caaccggaac aacggcatcc
     3601 gccggtttgc agaagaagaa tggcggttca cgatccggat caaagcccat ttcgcgggcg
     3661 tgagcggcat agttacgtcc gacgcagtaa acgcggcgca cggggaattg ttcatcactg
     3721 ccaacaaccg gtacggtaac gggcgcttgc ggttcgaata cgtattgagt cattttttct
     3781 ctcctaaatt aataacgcgc ttcgcggaac agccccaggg cttcttgtac cggtctgtcc
     3841 gaaaaactaa ataaaacgga atcttgcgtg gtctggaacg acacgccatg ccaggtcggc
     3901 accacgaaga tatcttttgc actaaagctg aaggtttcat tgccgatgat gacctgcccg
     3961 ctaccttcca ccacatggta gatggtgctg tcagtggtac gcgcaacgcg tgaggcaaac
     4021 cctttcggca gcagttgcag gaaagcgccc atcgacggca tcggatagcc gccggtgacc
     4081 gggttgacgt agcgcatttt gtagccatcc cactcgtcgg catcgcccag tcgggttaaa
     4141 tcgtgcagca cttcgcggct gcggtcataa cgatagttaa agatcggcga ggagttcccc
     4201 gtctgatgcc gcagcggcaa catattggca gcgtaacgcg gcagataatc tccctcttta
     4261 cgcgttaccg gttgttgctc ttccggataa tcttcggcaa aaccgcagcc cagaatattc
     4321 actaatggca gatccagccc atcgagccag ataaccggtt cgtcgccagg gttaccgtga
     4381 tcgtgccagc gccactgcgg ggtcaggata aaatcgcctt cattcattgg cgtgcgttcg
     4441 ccatcaacgg cggtaaatgc ccctttgcct tcaacaataa aacgcagcgc cgactgatta
     4501 tgacgatgac tcggtgccac ttcgcccggc atgatcagtt gcaaaccggc atataaagtc
     4561 gcggtaatgg aagactgccc gcgcagcgcc gggttttcca gcaccagcac gcggcgcacg
     4621 gcctctttcg caccaatcag gccgccgctc tccagtagca gcgggcggat ctcctgataa
     4681 ttccagtacg ctggcgcaca gttagcgttg ggcgttttcg gcaccagatg gtgcagtgac
     4741 tcccacagcg gggtcaggtt ttgcccggaa atatgctgat aaaattgctc gcggctattt
     4801 tgattgttat cagtcattgg gatgctcctt attcgttcac aacgcctgcg cgaggcagtg
     4861 agtcagggac agaggtcgac tggcgaacca cggtaatcag cagggttaac atcaccgcac
     4921 tgatcgccgc aggcacagcg ataatgaaaa acagggtgtc gaaagagaaa ttcatcgcca
     4981 tcatcacgcc gccagacagc gaaccaacaa tcgcgccgca gcggccgata gcatttgacc
     5041 agctcacgcc cgtggcgcga ctttgtgttg gataaagtgt tgcggtcagc gcattcagcc
     5101 ccacctgcga tccgctaatg ccaatgccag tgccgaaaat cgccagcgcc atcaaccata
     5161 atccatcctg gcttaagcca atcatcacga tgcatatagc gcctatggca tagctcagcg
     5221 ccagcacgcg gaaaggattg aacttatcca tcagcacacc cagcgccagc gcgcctaacg
     5281 taccaccaat ctggaacgcg gcggtgaccc aggacgcgtg ttgcaggtcg atgccgcgat
     5341 ggttcagtag cgtcggcatc cagcttgaga gcaggtaaat aatcagcagg ctcataaaga
     5401 acactaccca cagcatcaac gtgatgggta actgacgtcc cataaacagt tgacgaatgc
     5461 tgccttttgt aacggaggct gattcaatga gataaaagtg ggtatcgaca taacgctcgc
     5521 gggtgatggc gctgacggtt ttcgcaatca ccgcctgcgg tagctggcgg cgcacctgcc
     5581 agcgtggcga ttccggcaga actaccagca gtgcgacaaa cagcatcagc ggtaacacgc
     5641 cccccaacac cagaatgccg tgccagccaa tcaccggcac cagctgcgca ctgacaatcc
     5701 cgccaaacgc cgaacccagc gtaaagccgc aaaacatcag cgtcaccagc gcgccgcggc
     5761 ggcgggcagg cagatactcc gaggtcatgg tgatggtgtt cggcatcgcg ccgcccagac
     5821 ccaggccggt gagaaagcgc aggaagacca gcgtttgcag atccggtgag aaagccgagg
     5881 cgaggcttaa cgcgccaaac aaaaagacgc acagctcaat cacccgcttg cgaccaaagc
     5941 gatcggacaa cgggccacaa agcagtgcgc ctgcggttaa tcctagcaga cccgcgccaa
     6001 aaagcggggc gagatcgcct gcggtgagtt gccagtgagt gcgaatatca ggggcgataa
     6061 atccgatagc cgcggtgtca aaaccatcga gcattaccac cagaaaacag cagatgataa
     6121 cgcgccactg catcttgccg accggagcag catcgataag tgcttgtaaa tcacgtcgtt
     6181 gagtcatagt aaatgcctca gtgcaggtaa ggtagatgtt tcttttgttt tgtatatgtt
     6241 gcgatattgt gattaaagag atgagatgta caatggcatt tcgggcagag agataacctg
     6301 gaggttatgg caaatggcga actgggcgca aaaattaaaa ctacatcacc ttgaaatgct
     6361 ggtggcgctg ggtgaacagg gcaatctaac ccatgtcgca cgaatgatga atatcaccca
     6421 acctgcgctt tcgaaatggt tgtcacaact cgaagatgag attggaatca cactttttga
     6481 gcggcacagc aaaggattac gtccgactga aggcggcaaa ttgttattgc aacatgccca
     6541 acggttaatc aatgacatgg agcgatcgca acatgaactg gcccgtttta aacaaggtgg
     6601 actggtcggc agtctgaaaa ttggctgttc tcccgtcgcc actgactgcg tatcccaggc
     6661 gattttgcat ttgcttacga agatgccaac tctacacctg aatatcgaag agaaggtgat
     6721 gaccccattg ctgcaagatc tattggcagg ttctgtcgat gtggtcgttg ggcgaattgg
     6781 cggtcgcgca ttgcagctgc cactgaatta tcaggtgctg tacaccgagc cagtatgctt
     6841 tgtcgcccgc cctgagcacc cactggcgaa atacgccacc ctcagctgga atgatcttgc
     6901 caactggcgc tggatcgtct ggccgacggg tacccccatt cgcattagta ttgataatgc
     6961 gctggtagac cacggcgtaa tgctgccaga aaacacgatt gagtcggcgt cgatgaacgt
     7021 cagtaccaac ctgttacaaa gcagcgatat gatctctatt ttgtcattac gcctggcgca
     7081 acgttatgcc agccagggac aactggtgat actcaacctg ccaaagatag aacagaaagg
     7141 tagcgtcgga gtgttctggc gcaataacga aacgccttct tatgctttaa gtcagtttct
     7201 gcacttttta cagcagaatt agtgtgctga acggggcgct cgcccgaggt tacctcccta
     7261 aaattcaact atgctgagaa ttcttaatgt gatcggtagc acgttttacg aattaattgt
     7321 atgatgaatc catctcatct ggggtgttga ttatgagtaa gacactgaac attatctggc
     7381 aatatttacg cgctttcgtc ctgatttatg cctgcctgta tgcaggcatt ttcattgctt
     7441 ccctgctacc ggtaaccatt ccgggcagca tcatcgggat gctgatcctg tttgtcctgc
     7501 tggccttgca aattcttccg gcgaaatggg tcaatccggg gtgctacgta ctgattcgct
     7561 atatggcgct attgtttgtg ccgattggcg taggcgtcat gcaatatttt gatttgctcc
     7621 gcgcacagtt tggcccggta gtggtttcct gtgcaatcag tacgctggtg gtttttctgg
     7681 tggtgagctg gagttcgcaa ctggtacacg gtgaacgtaa agtcgtaggt cagaaaggat
     7741 cagaagaatg atggcgaata tctggtggtc attaccgctg acgttgattg tcttttttgc
     7801 cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt aacccgttgc tggtagcaat
     7861 ggtggtgatc attccttttt taatgctgac cggcatctct tacgacagtt actttaaagg
     7921 tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc gcgctggcct atcctttata
     7981 tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc atcaccatct gttttatcgg
     8041 cagcgtggtt gcgatggtga cggggacttc cgtggcattg ttgatgggcg cttcaccgga
     8101 aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct attgcaatgg cggttggcgg
     8161 cagtattggc ggtattccgg caatcagcgc agtttgcgtg attttcgtcg gcatcctcgg
     8221 cgcggtattt ggccatacat tgcttaatgc gatgcgtatt cgtaccaaag ctgcgcgcgg
     8281 tctggcaatg gggactgcct cgcacgccct cggtacagcg cgctgcgccg agctggatta
     8341 tcaggaaggt gcatttagtt cgctagcgct ggtgttatgc gggataatta cttcgctgat
     8401 cgcaccgttc cttttcccga tgattctggc agtaatgggc taaaatttgc gatgcgtcgc
     8461 gcatttttga tgtaagtttc acgcgttgca taattaatga gattcagatc acatataaag
     8521 ccataacggg ttcgtaaact gttatcccat tacatgatta tgaggcaacg ccatgcatcc
     8581 acgttttcaa accgcttttg cccaacttgc ggaaaacttg caatctgcac tggaacctat
     8641 tctggcagac aagtacttcc ccgctttgtt gaccggggag caagtctcat cgctgaagag
     8701 cgcaacgggg ctggacgaag acgcgctggc attcgcacta cttccgctgg cggcggcctg
     8761 tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt gcgcgcggtg tgagcggaac
     8821 ctggtatttt ggtgccaata tggaatttat tggtgcgaca atgcagcaaa ccgttcatgc
     8881 cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa aaagcgcttg cagccatcac
     8941 cgttaactac acgccttgtg gtcactgccg tcagtttatg aatgaactga acagcggtct
     9001 ggatctgcgt attcatctgc cgggccgcga ggcacacgcg ctgcgtgact atctgccgga
     9061 tgcctttggg ccgaaagatc tggagattaa aacgctgctg atggacgagc aggatcacgg
     9121 ctatgcgctg actggtgatg cgctttctca ggcggcgatt gcggcggcaa accgttcgca
     9181 catgccttac agtaagtcgc caagcggtgt tgcgctggaa tgtaaagacg gtcgaatttt
     9241 cagcggcagc tacgctgaaa acgccgcatt caacccgact ctgccgccgt tgcagggagc
     9301 gttaattctg ttgaatctca agggttatga ttacccggat atccagcgcg cggttctggc
     9361 agaaaaagcc gatgcgccgt tgattcagtg ggatgccacc tccgcaacgc tgaaagcgct
     9421 cggctgtcac agtatcgacc gcgtgcttct cgcttaagcc tggtgccgga tgcggcgtga
     9481 acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa atcccggcaa acagtttgcc
     9541 gtttcttgcg caaaaccagc gggtaaagta gcctgatgga aattttcctt agatcgagtc
     9601 tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc ttatcggctt gctgctgttg
     9661 actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg cgccttatat ctacgacgaa
     9721 ttgcaggatc tcccctaccg ccaggtcggt gtggtgctcg gaacagcaaa atattatcgt
     9781 actggcgtaa ttaatcagta ttatcgctac cgcattcaag gagcgattaa tgcctataac
     9841 agcggtaagg taaattatct attactgagc ggcgataacg cattgcaaag ttataatgag
     9901 ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg acccatcaga tattgttctc
     9961 gattacgcag gctttcgtac gctggattcc atcgtgcgta cacgcaaagt tttcgatact
    10021 aatgatttca ttattatcac ccaacgtttc cactgtgagc gggcattatt tattgcgctg
    10081 catatgggga ttcaggctca gtgttatgcc gtaccgtcac cgaaagatat gctgtcagta
    10141 cgtattcgcg aatttgccgc ccgtttcggt gcgctggctg acctttatat ttttaaacgt
    10201 gaaccgcgtt ttttagggcc gctggtccct attccggcta tgcaccaggt gccggaagat
    10261 gcgcaggggt atcccgccgt cacacccgaa cagttacttg aattacaaaa gaaacaagga
    10321 aagtagttat ggatgtacag cagttttttg tcgttgccgt ttttttcctt atcccgatat
    10381 tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc gattgataaa cgggttaaaa
    10441 atgcaccgga accggtgtat gtctggcgag caaaaaatcc cggactcttt ttcgcttata
    10501 tggtggcata tatcggcttc ggaattttat ctatcggcat gattgtttat cttattttct
    10561 atcgttaata ttttctcttt tctgttaatt attgagaata attattactt cactcaataa
    10621 gctgcggata taacattctg aaccgcagct atttgtgaat cctttcacag tttaaatttc
    10681 cccacgcgca tagccttaat atcagcacgc aatatttatt tagcgttcgc cttaattact
    10741 catagcatta aggaagatca catgccgcaa caaaattatc tggatgaact cactccggct
    10801 tttacgcctt tactggcgat taaagaagcc tctcgctgtt tattatgtca cgacgctccc
    10861 tgtagtcagg cttgcccggc acagaccgat ccggggaaat ttattcgctc aatctacttt
    10921 cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata atgccctcgg tgccgtttgt
    10981 gccagagtgt gcccgacgga gaaattatgc caaagcggtt gtacccgtgc cggtgttgat
    11041 gctcccattg atatcggccg cttacagcgt tttgttactg attttgaaca acaaacaggg
    11101 atggaaattt atcagcctgg tactaaaacg ctcggcaaag tcgcgattat tggcgcaggt
    11161 cctgccggat tgcaggccag tgtgacactg acaaaccagg gttatgacgt cacgatttat
    11221 gagaaagaag cgcaccccgg tggctggttg cgtaacggta ttccgcaatt ccggttaccg
    11281 caatcagtgc tggatgcaga gatcgcccgt attgaaaaaa tgggcgtgac cattaagtgc
    11341 aataacgaaa tcggtaaaac actcaccctg gagcaactga aagcggaaaa ccgcgcggta
    11401 ctggtcaccg tggggttatc aagcggttcc gggctaccgc tgtttgagca tagcgacgtt
    11461 gagattgccg tcgacttcct gctacgtgca cgacaggcgc aaggcgatat cagcattcca
    11521 caaagcgcat taattatcgg cggcggtgat gtcgcgatgg acgtagccag cacgctgaaa
    11581 gttctcggct gtcaggcggt aacctgcata gcgcgtgaag agttagatga gtttccggca
    11641 agcgaaaaag agtttgccag cgcccgggag ctgggcgttt cgatcatcga tggattcacg
    11701 ccagtagccg tggaaggcaa taaagtcacg tttaagcatg tacggttatc gggcgaactg
    11761 acgatggtgg cagataaaat tattctcgcc gtcggtcagc atgccagact ggatgccttt
    11821 gcggagttag agccgcagcg taacaccatc aaaacacaaa attatcagac ccgcgatccg
    11881 caagtctttg ctgctggcga tattgttgag ggtgacaaaa ccgtggtcta tgccgtgaaa
    11941 accgggaaag aagccgccga ggcgattcat cactatttag agggagcttg ctcatgttaa
    12001 cgaaagatct ttcgattact ttttgcggcg tgaagtttcc caacccgttc tgcctctctt
    12061 cttcgccggt aggcaactgc tatgagatgt gtgccaaagc ctacgacaca ggttggggcg
    12121 gcgtggtgtt taaaacgatc ggctttttta tcgccaacga agtctcgccg cgttttgatc
    12181 atctggtgaa agaagatacc ggttttatcg gcttcaaaaa tatggagcag attgctgaac
    12241 atccgttgga agagaatctg gccgccctgc gtcggctgaa ggaagattac ccggacaaag
    12301 tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg ggaggagctg gcgcgcctgg
    12361 tgcaagaagc tggcgcggat atgatcgagt gtaacttctc ctgtccgcaa atgacttctc
    12421 atgcgatggg tagcgatgtc gggcaaagcc cggagctggt agaaaaatat tgtcgggcag
    12481 tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac gccgaatatc ggtgatatgt
    12541 gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg cattgcggcg attaacaccg
    12601 ttaaatccat caccaatatc gatcttaatc agaaaatcgg tatgccgatc gttaacggaa
    12661 aatcgagtat ttccggatat tccggtaaag cggtaaaacc gatcgccctg cgcttcattc
    12721 agcaaatgcg cacccatcca gaactgcgcg atttcccaat cagcggtatc ggcggcattg
    12781 aaacctggga ggatgcggcg gagtttttat tgctcggcgc agcaacgtta caggtgacca
    12841 ccggcattat gcagtacggg tatcggatag tggaagatat ggcgagcggg ttgtcgcatt
    12901 atctcgccga tcagggattt gattcgttgc aggagatggt aggtctggcg aataacaata
    12961 ttgtcccggc ggaagattta gaccgcagtt atattgtcta tccccgtatc aatcttgata
    13021 aatgtgttgg ctgtggacgc tgttatattt cctgttacga cggcggtcac caggcgatgg
    13081 aatggagcga gaaaacccgc acaccgcatt gtaataccga gaaatgtgtg ggttgtctgc
    13141 tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg ggaagtgaag tttaagaaag
    13201 gcgagaaaga acacccggta acgttgtaaa accagagacg catccggcat ttggttcctg
    13261 tcgccggatg cggcttgaac gccttatccg gcctacaaaa ccatacaaat cagtatgttg
    13321 agatcattgt aggccggata aaacgcgcca gcgttgcatc cggcatttgg ctcctgtcgc
    13381 cggatgcggc gtgaacgcct tatccggcct acaaaaccac acagatcatg atgttatgtg
    13441 cgtttatgac cgaatgcgga ccacaatcat atcacttctt acgcgcgtac ttcagtgaat
    13501 ccagcgctac ggcgaagata ataatcgccc ctttgatgat gtactgccag tatgggttta
    13561 cgccgatata cgtcaggcca tagttgatga cggtaaaaat aattaccccg gtcaccacgc
    13621 caatcaccgt ccccacaccg ccgctgaacg atacgccgcc taccacgcac gccgcgatag
    13681 catccagctc atacataaag ccgaggttgt tggtggcaga gccgatacgt ccggcttcta
    13741 acatcccgcc aaaggcatag aacacgccag acaacgcgta gatcatcagc aggttcaggc
    13801 cgacgttgac accagatact tttgccgctt ccgggttacc gccaatggca aaaatgttct
    13861 taccgaagcg ggttttgttc cacaacaccc agacgaacgc caccgcaatc aacgcgtaga
    13921 aggtgatgta agagagacgg aaactcccca gcgcgacaaa gccctgagca aaggtagaga
    13981 agccactgtc aaaaccagaa attggcgacg ccccgacgaa atcgtaatag agcgagttga
    14041 tgccatagac gatgatcatc gtgccgagcg tggtaatgaa cggcgtaacg ttgagataag
    14101 cgataatcag accgttgatc aaaccgatca ccgcaccaat ggcacagaca atcagaataa
    14161 ccagcgcaat cggcatcgtc gccatttccg ggaacacttt gttggcgtta tccatggact
    14221 gcaataatgt cgctgccacc actgccgcca gccctacttg acgccccgca gaaaggtcgg
    14281 tcccctgggt gacaattaac cctgccacac cgagcgcgat aataatacgc accgatgact
    14341 gggtgagaat attacttaag ttcaacagac ttaaaaatgt tgggtcctgg aaaataataa
    14401 tcgccagcaa aactaaaaga acgacgtaaa taccgccctc tttcaggtaa gtaagaaaac
    14461 ttttcttatt taacgcactc atgggaagcc cctaatctta aaggtgcaaa gacgcaagac
    14521 gcagaatttc gttttgcgtt gttgtttttg tatcgacaat tccggaaacg agaccattgc
    14581 tcatgaccag aatacggtct gttatcccta acaactcagg catttcagag gagataataa
    14641 taatcccttt gcctttcttc gccagttcgg caattaactg atagatctca aacttcgccc
    14701 cgacatcaat accgcgcgtc ggttcatcga gcattaatat ttccggttgc gttagtagcc
    14761 agcgcccaat aatcaccttt tgctgattac caccggagag cgaaccaatt tgcgtccgat
    14821 gccccggtgt ttttacccgc atcgaatcaa tcacccactg ggtatcgctt ttcatccgcg
    14881 agttatccag taaaccaact ttatttttgt agttgcgaat attggaaatt aacgagttaa
    14941 aaccaatatc cagataggca taaattcctg ttgagcggcg ctcctcagtt accagtgcaa
    15001 atccgtggtt tatggcttcg ttggcattat ggttattgat ctttttgccg tgcaacgtaa
    15061 tggtgccagc cgatttctcg cgaataccaa ataacgtctc aacaatatcg gtacgtttcg
    15121 cccccaccag accggcaata ccgaggatct cccctttatg cagatcaaac gagacatcac
    15181 ggatcgacgg ctggcgcagt gacgtcaggt tacgcacctc gaggatgact tcgcccggct
    15241 tgttttcttt gtcagggaaa cgctggttaa gagaacgccc aaccatcatg gcgatgatct
    15301 tatccatcgt cagtcctgcc agcggttcgg tggcgatcca ctgaccgtcg cgcaatacgg
    15361 taacttcatc acataactgg aagatttctt ccattttatg cgagatataa acaataccgc
    15421 agccgcgctc ttttaattta cgaataatag tgaacagatg attgacctct ttttcggtta
    15481 acgaagaagt cggttcatcc ataatcacaa ttttcgcgtt ataggaaaac gctttggcga
    15541 tttcgatcat ctgcatttgc gaaacagata atgtgccgac gcgcgcacgc ggatcgatat
    15601 caatatccag ttcatcaaaa atcgctttgg tttcgcggta cattttgtcc tgatcgacaa
    15661 acatgccttt ggtgggatat cgccccagcc acatgttatc catcaccgaa cgttgtaata
    15721 ccaggtttaa ctcctggtgt accatcgaaa taccattttc cagggcttct ttggccgaat
    15781 ggaaatcgat ctctttaccc tggaataaaa tggtgccgga gtctttttga taaataccaa
    15841 acaggcattt taataatgtc gattttcctg caccgttttc ccccattaat gcatggatag
    15901 aatgtggccg gacttttaaa ttaacgttat caagtgcctt aacaccagga aaggacttgt
    15961 tgataccgct catttccaac aagtattccc cggaagacgg agtcgttgag ctgaccatat
    16021 aattttacct tgttggccat acaataaggg cgcagtaata gactgcgccc aatcagtctt
    16081 atttcttgct gaattcagcc aggttgtctt tatctacgcc aacataaggt acgcggacca
    16141 ctttgttgtc gattttccag ttggtgccat cagccgcacc tttaccatcg gccaggtttt
    16201 tcgccagatc aaaggtcgct ttcgcctggt tgttagcatc gttcagtacg gtgcccgcca
    16261 gtgcaccgga tttcaccagc gccagcgctt ctggcagagc atcgacgcca aacaccggaa
    16321 tgctggactt gttgtgtgct ttcagtgctt ctaccgcgcc cattgccatc gcatcgttgt
    16381 tggcgataac cacttcgatt ttgttggcgt tcgggccaga cagccaggcg tccatcttat
    16441 ctttcgcctg agcggtgtcc cacattgcgg tatctaactg taactgttca gttttaatgc
    16501 ctttgtcgtt cagctcttta atcacgtaag tggtacgtgc ttctgcatcc ggatggcccg
    16561 gttcaccttt cagcagtacg aattgaatct gaccgtcttt attcagatcc caaccctgat
    16621 tcgccgccca gtgtttagca atcaaatcgc cctgaataat gccggactct ttggagtcag
    16681 tgccaacgta gtaggctttg tcgtagctat ccagcgcctt acgagacggc tctttgttaa
    16741 agaaaaccac cggcacgttt tgcccacgcg ctttctcaat caccgtaccc gcagctgccg
    16801 ggtcaaccag gttgattgcc agtgccttca cccctttcgc cagcaatacg tcgatctgat
    16861 cgttctgctt ggactggtca ttctgagaat cattcatcag cagctgaaca tctggcgcgg
    16921 ctttcgcatc ttgctcaata gccttgcgca ctacagacat aaagttatcg tcgtacttat
    16981 agattgttac accaatgcga gtatcagcag cgtgtgcagc ggcaccgaat aacatgctgg
    17041 ccatcacagc agacagggtt aacaccttct tattcatggt atctccggtt tttcttatgc
    17101 agggtagtgc ttgagataaa tgctcggcgg ggcagtagag ttaatgaagt gttactgaac
    17161 gccgaagctc actttttaaa attcgttctt ccatgcccgg taacgctcca gaaaacggct
    17221 ttaattgttg ttatgacgct attacctcgg tgaaagtgat tattcactgt tacatacggg
    17281 ttacaacgtt aaaacggtgc aatcatagct atcacattgt taagatactg tgaaatcact
    17341 cacagattga aagcggttac atcgcctgat ttgttgagtt agtgatcgcc gccgcattct
    17401 ggcgcgttgc gacagaatgg cgacgcacta acgtcggcat aaaacagtgg ctggcacgag
    17461 gatcaatatt gcctgctgca ccctgcaagg ccagttcggt ggctaattta gccattgaag
    17521 caatgggata acgcacggtc gttaattgcg ggtcggtgta acgggcgatg ggaatatcat
    17581 cgaaaccgat gattgagaga tgtaacggaa tcgcaatgcc attatctttt aatgctgtca
    17641 gtgcgccagc agccatattg tcgttataag caaatacagc ggtaagttgt agattgcgcc
    17701 ccagcagttc aaccatcgcc gcctcaccgc ccggcatgtc cggcgtacca gtgccaatcc
    17761 agctttccgg cggaataata tcctgctctt tcaacgcact catccagcct gctttacgca
    17821 tggcgtcatc ttcaatgccg tggctggaag aaagataacc aatacgttga tgaccgttat
    17881 tcagcaacat gcgcgtcgcc attcgggcac cgctgagatt atccaggcaa acgcaacgat
    17941 gggcgtaccc cggcacaacg cggttgatta acaccatacc gggaatgtta tccataaatt
    18001 gcgccagttc atcgtcactc aatgcttttg agtgaacaat caatgcatta caacgctggc
    18061 gaattaacac ctcaatggcg tgacgttctt tttccgcttc atgatagcta ttgccgatta
    18121 gcacgtattt ctgatgctgc tgggcgacca gatccaccgc ttttaccagc gcgccgaaaa
    18181 acgcatcaga aacgtccatc accaccacgc caatggtgtc gctaacctga gttgccagcg
    18241 cctgggcatt ggcgtttggc cgataatcca gctcactcac ggctttcatt actgcttcac
    18301 gcgtgtcggc actgaccagc gtgctgttat tgagcacccg ggaaaccgtt gccacagaga
    18361 cgccagcctg acgcgctaca tcacgaatgg tgatcatatt cactacctgt tatgagattg
    18421 cagtaaatga ctgcttgctg gcggctattt tgtcagcact gaatacagga cttcgtgaat
    18481 cgagtcacag caatggaaac ggttacagcc gttttgttaa cgaatgtgat ccagatcgtt
    18541 atcttgatgt tttagatatt gccggtccgg cagcacgcaa agttaactga cgccagagcc
    18601 attccaccgg cccctggcgg aagtaacgca gccagataac agagaagaga atattcgcca
    18661 gccataccgg aataacaaac gccagcaatc ccagacggtc aaactgcgta aacaaaccga
    18721 ggtgataaaa aagcgtggta caaatcagcg tttgcaatag atagttggtc agcgccatcc
    18781 gtccgacgca ggcgatcgca agcaccagct taaagcggct caattgcggc cagaaaccat
    18841 aaaacagcga cgcatagcca atcgcctgaa acggtgcact cagttcccgc ggcatctgaa
    18901 gtaagaaggc gcaccagcga tatgcccagt ccagccgcca ttgcagggcg atggcaggaa
    18961 ggttaatgat caccccaatc gccaccagca caaaaccagt acgacgatag tgacgtaagc
    19021 tgaactgccc tttcagccag ccactgcgca tcaatgcggc accaatgagc atcatcccag
    19081 ccagttgcca gccatactgt gcgcccagtg ccagtaaact gttgccaaca ccatcggcac
    19141 gattactgat cgcttcaacg ccgccgtgaa gcttccagta tttttcatac aaaatagccg
    19201 atgcatccgg cgtccaggcg cggctggtct gggtatcgga aatcagcccc aataacagca
    19261 aaacgccaag ccccaccaga taaagcatga cgccggtatt aaacaggctt tttaccgatg
    19321 gcgcatcgcg caccagccgc cagcagatta agcccaccag cccgtaagcc agcagaatat
    19381 cgccgtccca gaacaataaa ccgtgaataa agcccagcaa gactaacagc gttaaccgcg
    19441 actggatcca gcgtctgcca cggggcagca acatttgcag gccagcacca aacagcagcg
    19501 caaaaagcgt gaggaatttc acctggccga tgagatcgag aaatgcccag gtccatgcat
    19561 cctgcggcgt aatagcgccg taccaggcgg gattaagata agccgccttt ggtagcccaa
    19621 aggcgctgat gtttaatagc aggatcccca gaatggcgac gccgcgaaca aaatcgagcg
    19681 tgacgttgcg ctccatggtt cctgcctttt aatcagttgt gatgacgcac agcgcgcaga
    19741 aactcgtggc gcgtattctg actggatttg aacaatccac caagagaggt cgttgtcgtg
    19801 gcactggttg catcgcggat gccacgcgcc ttcacgcagt aatgcaccgc gtcgatagag
    19861 acagccacgt tattggtgcc cagcagcgtt tgtagcgcaa taagaatttg ctgcgtcaga
    19921 cgttcctgca cctgcggacg ctgggcaaag aactgcacga tacggttaat ttttgacaga
    19981 ccgatcaccg aatctttcgg gatataagcc accgtcgctt tgccatcgat ggtaacaaaa
    20041 tggtgttcac aggtactggt cagagtgata tcgcgcacgg tgaccatttc atcgaccttc
    20101 attttgtttt caatgagggt gattttcggg aagttggcgt aatccagacc ggagaaaatt
    20161 tcatcgacat acattttagc gatgcgatgc ggcgtttcca tcaaactgtc atcagccagg
    20221 tcgagattca gcagctgcat gatttcggtc atatgaccag caataaggct tttgcgcgtt
    20281 tcgttatcca tttcatgcac gggcgggcgc agcggtgttt ccagtcctcg cgcaactaac
    20341 gcttcatgaa ccagggccgc ttctttactg agtgatggca tttatgattt ctcctgcagg
    20401 tgtgacgcct ccgccctgcg tgggggcaaa gttattaagc tgatttacag cctgattatt
    20461 gtgcgtgagg cggcgcacat aatccagtat tcacagcgat aattattgta attgccgctg
    20521 cctttcatca gcagatgtta aaacatcgtt atgcaaatac ggaagtgaaa gttactcaca
    20581 gcacattgaa taaacggtat gatgaagaaa ttgcaaacaa cacaacaagg agccacgcat
    20641 ggaaatgctc gaagagcacc gctgttttga aggctggcag caacgctggc gacacgactc
    20701 cagtacctta aactgcccga tgacgttcag tatctttctc cccccccctc gtgatcacac
    20761 cccgccacca gtgctgtact ggctttccgg attaacctgc aatgacgaga acttcaccac
    20821 caaggcgggt gcccagcgcg tggcggcaga actggggatt gtactggtga tgccagacac
    20881 cagcccgcgc ggcgaaaagg ttgccaacga cgatggctac gatttaggcc agggcgcagg
    20941 cttttatctt aatgccacgc aaccgccgtg ggcgacgcat taccggatgt atgattatct
    21001 gcgcgatgaa ttaccggcgc tggttcagtc gcaatttaat gtcagcgacc gctgcgctat
    21061 tagcggtcat tcaatgggtg gtcacggtgc gctgattatg gcgctgaaaa acccgggcaa
    21121 atacaccagc gtttccgcct ttgcgccaat tgtgaatccg tgcagcgtcc cgtggggaat
    21181 caaagcgttt agccgctatt taggtgagga caaaaatgca tggctggaat gggacagttg
    21241 cgcgctgatg tatgccagta acgcgcagga tgcgatcccg acgcttatcg atcagggcga
    21301 taatgatcag tttcttgccg atcagttgca acccgcagtt ctggcagaag ccgcgcgcca
    21361 gaaagcgtgg ccgatgacgc tgcgtattca gccgggatat gatcacagtt actacttcat
    21421 cgcctctttt atagaggatc acctgcgctt ccatgcgcag tatttactga agtgaaagtc
    21481 cgcccggttc gccgggcatc ttctcatcag aagcgataat ccactgccat aaagtaacga
    21541 cgtccgtctt cgttatagct gtagtcgtca cgactgagat ccttgtcgcc aaggttcagc
    21601 acgcctgcgc gcagtttgac gtctttagtc acctgccagg ccgcgccggt attccagatg
    21661 gtataaccgc ccggtgtttt agccgtcgcg ctgtcggctc gtttctgccc ggtatagtga
    21721 ccagaaacat agaatgacca gtcttccagc gccagcggtt tccagtccag cgtaccgttg
    21781 gcggtatgga acggcagatc ggatagcggt ttgttttcgc cgttgctgac atcacgacca
    21841 tcgttgtagg tgtagttgag tgacagtttc cattcatcgt tgaacggaat tttcagctcg
    21901 gtttccacgc cctgaatacg tgctttgtta acgttgtagt agctaaatac cggcacaccg
    21961 ttcgctgtaa aaccaacaaa gttttggtag cccggtgcag cattgacgtc agacgtacgg
    22021 ctgatgctga tacgatcttt cacatcgtta cggaaaacgg taacgctgga ttcaacccct
    22081 tccagccagc cttcttcacc catgtagtac agccccagct cccaactttc gctggtttct
    22141 ggtttcagat ccgggctacc cacaatctta catgcgccac ggcaggaatt gctcgtccag
    22201 tcagggctaa gttgcaacag agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc
    22261 gttacggtgt cggtggcgtt ataaaccagg taggcacgcg gactccagtg ttcaccgtag
    22321 gtttcgtgat cgtccatacg cacgccggtc gtcagcgcca gcggctcgaa gatccgccat
    22381 tcatcttcca caaacagcgc gtactggctg gcagacgttt tggagctggt tcccccggtc
    22441 aggttcaccg catcgctaag tttgtcgtga cgccattcac cgccaaccgt gagaaactga
    22501 ttaatcgccg tcagcggcaa cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc
    22561 gggctgctgt tgccagggtt tttgttctcg actttctcac cgtagtattt cagttcgctg
    22621 gtgccgtaat cccaacgccc attatggctg acggagtagt tctggcgttc caggcggttt
    22681 ttgtccagcg agtcggaatc acgatcctga cggtcgaaac cgtatccggc agtaaaatcg
    22741 tgattttgat tcggtgtcca ggcaaattcg acattgccgt cgcggctgga gaatccttca
    22801 atacgcggcg tttctccggt atcggtggtc gttgagtttt gcgggtcatc cttttcacgt
    22861 tttgccaggc tgccgtaagc tttcattccc agcacaccat caattaatgg tccactggta
    22921 aagaactgac cgttataggt atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg
    22981 gtaacggtac ccgaccattt ctgaccgatt tttttggtga tgatattcac tacaccgccg
    23041 agcgcatcgg aaccgtacag cgacgacatc gggccacgga ccacttcaat acgttcgatg
    23101 gaatcgaccg ggatccagtt cagatcgaaa tcattgtggc ggaagacggc attgcgggag
    23161 ttaacgcgtt taccgtcgac aagaatcagg gtgtagctgc tgtccagacc acgaatacta
    23221 acgcccttac ggttatcccc ttcgttcgtc agttgtacgc caggcacttc tttgaggaca
    23281 tccttcagat tctgtaccgg ttttcgctgc aggtcttcct gggtaatgac gctgatactg
    23341 gcgggtgcat ctttaaggtt ttgttcaacg gaagatgcag tgacaaccat cgtttcgcca
    23401 tcatcatcga ccgctaacac aggccatgca caagaaatag cggacaaaca cagcccgacc
    23461 cgtacgaaag ggttcaacct aaacattcca tatctccatg aggtaactac gaaaataaaa
    23521 tgggttatcg ctcacatctt cttcacgtcc ccttgcgtac ggcagcatcg cggtggactt
    23581 atttgtatgc aggtgatcat cccagaaaac cggaccagcc tctgatttgt cagccttttt
    23641 tgattgcggc gtaacgataa tgcaaacgat aacaattatc aatccaaatg ttaaatttta
    23701 tatccgcatg atttgtcagg gaaatggaat tgaaaaagcc ctctcggttg agagggctta
    23761 gcaaggaagg gagaaactta tttcttatcg ttctgcggga acttcatttc gctgtagcgt
    23821 acgaagtggg ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata
    23881 ccaatatacg ttgccgctac accgccccag tcaatggtat ctttcaggaa tgcttcgtag
    23941 ttctggccca aagtgataat cagacacaga atgaatgcga agatcggacc cagtgggaag
    24001 aaacctgaac ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg
    24061 aagcgatagt ggctaatggc aatccccagc caggcgataa aaccggtcat cccggaggtg
    24121 ttcagcagcc acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacagaccg
    24181 gcaatcaccg tcgtcgcata cagcgcatta cgcggcacgc caccacgcga cagtttagcg
    24241 aaaatgcgcg gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac
    24301 ataccggagt tacccgctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc
    24361 gcagagagca gacccgcgtg ctggaacacc agggtgaacg gactaacgct gatgtcttta
    24421 acatcgttac gtagcaggct tggatcggtg tacggaataa tcaggctgat aatcaggatc
    24481 gcgaacacat agaacaacag gattcgccag aacacctgac gtaccgcgcg tggaatgttt
    24541 ttccccggat cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag
    24601 aagccgacaa tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg
    24661 ccgattgtcc agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc
    24721 agcacgccaa cgatgataaa gacaataact gtcgtgactt tgatcagtga gaaccagtat
    24781 tccgcttcac caaagccgcg aacagagatg tagttcagca ggaagataac gccgaggaac
    24841 aacgcactcc agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgagct
    24901 gcaaccaggt caacggcaat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg
    24961 aagccaaagc cctcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc
    25021 atatatgcag ccagttcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc
    25081 atatacgaga gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca
    25141 aaaagacctg taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat
    25201 tcacggcgta agcccggcgc ttctgtggtt ttagtttcgg aaaccataca aaaatgctat
    25261 ccatcttaaa aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac
    25321 acggttataa gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt
    25381 tctggcgaat gcatgctgaa ggcgctgcgt cacgaaatcg acacagcacc agcatgttct
    25441 tgtacagcaa cttaacgcgg gacattcgcg ggatcgcaat agtccagaaa gcgccgtagc
    25501 gcgttggaaa ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggc
    25561 agagggaccg caacttcact taatgtgcct gcctgcaatt ggtcttcaat cacccgtcgc
    25621 gacaggcaac taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct
    25681 aattccatcg ccatctcaaa cttcggtaaa tgtgacagca acagataatc gacaatctcc
    25741 cgcgtgccgg agccgcgttc acgcaggatc cacggcgcag cggccagctg ttctaaggtg
    25801 accggaccac gggccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc
    25861 catggttcag aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg
    25921 cggaaatcca gcaccgcttg catcacgtcc tggctgttcc caacgctaag ttcaatcggc
    25981 aactgcggat aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg
    26041 gtactactgg catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca
    26101 accgcctgtt caagcagtgc caacgcacgc ggatagagca gccgcccgtg ttcattaaca
    26161 accagtcttt tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc
    26221 aaggctgcgc tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat
    26281 ccacttttca atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct
    26341 taccacttat aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc
    26401 gtaaccttat gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca
    26461 tcgtacactg tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc
    26521 cctgtggggt ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc
    26581 aatcttgttg gggatggttt taggcaacac catctatccg cacatctgga aaagctgtga
    26641 cggtggtgtg ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt
    26701 ccgtctgacg ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt
    26761 gacgctgtcc agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga
    26821 taagcacacc agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact
    26881 ggcgactgag ccggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt
    26941 tgttatcttc gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca
    27001 atggtttagt ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca
    27061 ggtggtggcg gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa
    27121 aatgctgcgc gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca
    27181 gctgtctggg gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt
    27241 gttcatcgta gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat
    27301 gctggtaacg ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac
    27361 tcacgtcagc gcgctgaaaa aagccggggc gaaacctctg ctgatggcac tggtactgtt
    27421 tgcctggctg attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata
    27481 aaccactaca tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta
    27541 acaggagtcc tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa
    27601 atgccgcaat tcgcgccgcc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc
    27661 aacgtcagtg gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct
    27721 gtgaaaaata tcactacaca tcggcgcaaa ttcttcccca cgacagctat ctgattaacc
    27781 tcggacatcc ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc
    27841 agcgttgcga acagctgggg ctttctttgc tcaacttcca ccctggcagc catctgatgc
    27901 agatttcaga agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata
    27961 aaactcaagg tgtgaccgcg gtgattgaaa acaccgccgg tcagggcagt aacttagggt
    28021 ttaaattcga acatctcgcg gcgattatcg acggcgtgga agataaatcc cgtgttggcg
    28081 tctgcattga tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat
    28141 gcgagaaaac gttcgcggat tttgcccgta ttgtcggctt taagtatctg cgcgggatgc
    28201 accttaacga tgcgaaaagc acctttggca gccgcgttga ccgccatcat agcctcggtg
    28261 aaggcaatat cggtcatgat gcgttccgct ggatcatgca ggacgaccgc ttcgacggca
    28321 ttccgctgat ccttgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga
    28381 aagcgcaaca aactgaaaaa gcggtagcct gaagatgaat aaccgggaaa aggagatcct
    28441 tgcaatttta cggcgtaacc cgctgattca gcagaacgaa attgcggaca tgctgcaaat
    28501 cagtcgttcg cgcgttgcgg cgcatattat ggatttaatg cgcaaaggcc ggattaaagg
    28561 caaaggttac attctcaccg agcaggaata ctgcgtagtg gtggggacaa tcaatatgga
    28621 tattcgcggg atggcggata tccgttaccc gcaatcggct tctcatcccg gtacaattca
    28681 ttgctcagcg ggcggcgtgg gacgcaacat cgcccacaat ctggcgctgt taggccgcga
    28741 cgtccatttg ctttcagtga ttggcgatga cttttatggc gaaatgctcc tggaagaaac
    28801 gcgccgtgcc ggcgtgaatg tctccggctg cgttcgtttg catggtcaaa gcacatcgac
    28861 gtatctggca attgccaatc gagacgatca aaccgtgctg gcgattaacg atacccatct
    28921 gctggaacag ttgacaccgc aactactgaa cgggtcgcgc gatttacttc gtcatgcggg
    28981 cgtggtactg gctgattgca acctgacagc cgaggcgctg gaatgggtct ttaccctcgc
    29041 tgatgagatc ccggtgtttg tcgataccgt ttcagaattc aaagcgggca aaatcaaaca
    29101 ctggctggcg catattcaca ccctgaaacc cactttaccg gagctggaaa ttttatgggg
    29161 acaggcgatc accagcgatg ctgaccgtaa tgctgcagtg aatgcgttgc atcagcaagg
    29221 tgttcagcaa ctgtttgttt atttgcccga tgagtcagtt tattgcagcg aaaaggatgg
    29281 agaacaattt ttgctgaccg caccagcgca tacgacagta gacagttttg gtgctgacga
    29341 tggttttatg gcgggcctgg tatatagctt tctggaggga aacaatttcc gtgacagcgc
    29401 ccgttttgcg atggcctgcg cggcaatttc acgcgccagc ggcagcttaa acaaccctac
    29461 cctgtctgcc gataacgcgc tttcattagt gccaatggtg taacaatgtt gccggattcg
    29521 acgcttgacc gcatcttatc cggctcataa atcgcacact acgctaaacc aataaagaac
    29581 ccggcaatgg tggcactcat caggttagaa agtgtcgccg ccgccagcgc gcgtaaacca
    29641 agctgggcga tttccggcgc acggtgtggc gcaaccgcag aaaacgcccc caccaccacc
    29701 ccgatagaac caaagttagc gaaaccgcac aacgcgaagg aaataatcgc cacggttttt
    29761 gcatcgagag tgccagctgt ttgcagatag ggtgagaaat tgagataagc gacaaattca
    29821 tttattgcca gtttctgtcc aatcaaactc ccggcaagat tcgcatcagt ccagtcaaca
    29881 cccattaccc acgccagtgg ggccagcagg taacctaaaa tggactccag cgaggcatgt
    29941 tcaaaaccaa accagccgcc aacgccgccg ataataccgt taattaacgc aatgatggca
    30001 acaaatgcca tcaccactgt tgccacaccc gcagcgattt tcagcccggt caccgcccct
    30061 gtcgcggcgg cttcaataat gctttttggc ggtgtttcgg tgaaagagag gttattaaag
    30121 gaaacctgtg aagattccgt agccgggctt agcatgcggg caaacaagat cccgccgggg
    30181 atcgccatta acgatgccgc cagcaaatat tccacaggca cgcccagtgc ggcgtaacca
    30241 atcatcgtcg aaccagcaat tgaggccatg ccactacaaa tcgctgtaaa taactcattg
    30301 cgattcagcc gatcgataaa gggtttgacg attgccggaa tttcgttttg cccgaggaaa
    30361 atggtagtga ccgcgacgaa tgactcgatc ttgctgatat ttaatgcttt ctggaatata
    30421 ccgccgagaa tgcgaattaa gatccccatc acaccgatgt agtagagaat actcaccagc
    30481 gcggtgacga agataattgc cggtaacacc ctgaaaccaa agataaatcc tgcgccatca
    30541 aataaggtgt ccattttcgg tccgaccaga gaaccgaaga taaatgcgct acccgcgtcg
    30601 ctgtacgcca tcactttatg cacgccaaaa gcgacttttt cagcgaccca acgccctggc
    30661 ggtaaccaaa gcataatgcc gccaatcacg acctgtaaca ctaacgccgc gccaacggta
    30721 cgcaggctga tcttcttctt gtttactgac agtaaaaacg caatcgtcag caataccacc
    30781 attcccagaa cacttctcat gacatccata gttattatct cttcatgtca tattgggcga
    30841 cggctttctg cccctcagaa atagccgggc ctgcatcttg cgatactgac ccggcttgtt
    30901 atcaacactt aatgggtttt gatgtagccg cgcacgcact cttcgactaa gccccagaac
    30961 cagtcggtat caatagtgat gccgactttg gtattagcgg gcttgccaag aacgcccagc
    31021 tcgtcgcaga cggtacgccc atagcaaggg ccactgttga cgtccacttc gacgtacatc
    31081 tcctgggttt taatgccatc agggttaatc agataaccga tgcaggtggc gtcgtgcacc
    31141 gggccgccag ccaggccgta gttttcgaac tgggttttga gagtgaagtt catgatgtcg
    31201 ctgaacagct ctccggcagg gccgcctgcc ctttccatcc gagcaattac gtccggggtg
    31261 caaacggtct ggttggtaag atcgaggccc atcatcacta atggaacgcc ggaggtgaac
    31321 accacgcgtg cggcttccgg gtcagcaaag atgttgaact cggcagatgg cgtgaagttg
    31381 cctgtaccgt aagcgccgcc cattagcaca atttcacgga ttttgggcag gattgcgggt
    31441 tgcatacgca ttgccaccgc gatatttgac aacggaccaa ccggcaccag agtgatatcg
    31501 ccatcgcttg ccatcagggt atcaatgatg tatttcaccg cgtgggtact ttctgcctgg
    31561 cgggtcagcg gctcgaatac cgggccatcc agtccggttt caccgtgaat attatcggca
    31621 acgatttgtt gacgcataat gggttgcggc atccccgcat aaaccggaac attaatctcc
    31681 agtttctggc aaacattcag gccattaatt aatgttttat caagcgtctg attacccgca
    31741 acaatggtga tacctaataa atcaatcgcc ggatgtttcg ccgccatcat tatagcaata
    31801 gcatcatcat gacccggatc acaatccaga ataatttttc tcttttccat tgtttatttc
    31861 ctctgtttcc agttgcgtta ttttttctac agcaaagaaa tttcgatcgg gccgatgatt
    31921 gaatcttaac aacagcgtac gtatgctaaa tatgagaaat ctcatagcgg ataaacatcg
    31981 tgaaagaaat ccacaataat gatcttaagc agcaattgat gagtgaatcc gcgtttaagg
    32041 attgcttttc aacggatgtt tcagccgata cgcggctgtt tcatttttta gcgcgtgact
    32101 acattgtgca ggaagggcaa cagccgtcct ggctgtttta cctgacgcga ggccgcgcca
    32161 ggctttacgc cacgctagct aatggtcgcg tgtcgctgat cgatttcttt gccgccccct
    32221 gttttattgg tgagattgag ttaatcgata aagaccatga accgcgtgcg gtacaggcta
    32281 ttgaagagtg ttggtgcctt gcgctcccta tgaaacatta ccgtccgctg ttattaaacg
    32341 acaccctatt tttacgaaaa ctctgcgtta ctttaagcca taaaaattat cgcaatattg
    32401 tttctttaac tcagaatcaa tcatttccgt tagtgaatcg cctggcagca tttatattac
    32461 tctcacaaga aggtgatctt tatcacgaaa agcatacgca agcggcagag tatttaggcg
    32521 tttcttatcg acatctttta tatgttctcg cgcaattcat tcacgacggt ttattaatta
    32581 aaagcaagaa aggatatcta attaaaaaca gaaagcagtt gtcagggctg gcgctggaga
    32641 tggacccgga gaataaattc tccgggatga tgcagtaaaa attatttgca atagcgcgat
    32701 tgccggatgc aacgcttaat aagcgagtac atccggcaat gaaactcaag ctaaaccaat
    32761 aaagaacccg gcaatggtcg cgctcatcag gttggaaagc gttgctgctg ccagcgcccg
    32821 taaaccaagc tgggcgattt ccggagcacg ttttggcgaa atagccgaaa atgccccaac
    32881 gacaacaccg atagaaccaa agttagcaaa accacaaagc gcaaaggaga taatcgcaat
    32941 ggttttcact tccagcgtgc cgcccgtttg caggtagggg gagaaactca ggtaagcgac
    33001 gaattcgtta atcgccagtt tctgcccaat caggctaccc gcaaggttgg catcactcca
    33061 gtccacaccc atgatccacg ccagcggtgc cagcacatag ccaaaaatac tttccagaga
    33121 agcattggta aaaccaaacc agccgccaat tccgccgata atgccgttga tcagcgcaat
    33181 aattgcgaca aacgccatta ccaccgtcgc cacaccggcg gcgattttta gcccggtcat
    33241 cgcaccgctc gccgccgctt cgataaagct ttttggcggc gtttcactga acgacagatt
    33301 ttcaaatgtg acctgcgaag gctcggttgc cggactaaga atacgtgcaa acaaaatacc
    33361 gccggggatc gccatcagcg atgccgccaa caggtagtca attggtacgc ccattccggc
    33421 ataaccaatc atcatcgaac ccgcaatgga cgccatcccg ctacaaatag ccgtaaacaa
    33481 ctcgtttcga ttcatgcgat cgataaacgg tttaacgatc gccgggatct cattttgccc
    33541 gaggaaaata gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg
    33601 gaaaatgctg ccaaggatgc gaattagcag ccccatcacg ccaatgtagt acagcagact
    33661 aatgagcgca gtgacgaaga taatcgccgg gagtacgcga aaggcgaaga taaaacccgc
    33721 accgtcaaag aggacatcca ttttcggccc gaccagcgaa ccaaaaatga aggcgctacc
    33781 ggcatcactg taagacatca ctttatgaac gcctaatgcc gcctgttcta ctgcccattt
    33841 tcccggcgga aaatagagca taatgccacc aatagcgatt tgcagcagca gtgcggctcc
    33901 aaccgtgcgc aaactgatgc tctttttatt taccgacaac agaaatgcta ttgccagtaa
    33961 caccaccatc cccacaacac ttctcattat atccataatg attttccctt catgccagta
    34021 aacccggcgt cagcgccagg ttttggtatg tttgatgagt acgggcgacg gctttctgcc
    34081 cgtcagaaat taacccgcga gacgctgata ttctttggca atttcgctcg ccagaatggc
    34141 gttgttgaac accagctgga tgttggattt caggctgtca ccgccggtca gctcagcaac
    34201 gcgcgccagc aggaatggcg tactttcttt accaataaca ccctgcgctt cagcttcagc
    34261 taccgcctga tcgatcgccg cattgatagt gtgttccggc atagcaaact gttccgggat
    34321 cgggttcgct accacgaggc caccgttcag cccgctttgc catttcaccg ccattgcacg
    34381 ggcaatttcg ctggcgctgt cgagacgaat gctgacgtca aacgggctgg tgcggcagaa
    34441 aaacgcaggc agcgcttttg tctgatagcc aattaacggc acaccgaagg tttctaaata
    34501 ctcagtggtt aatcctaaat cgagaataga tttcgccccg gcacaaacaa cggtgacatt
    34561 agtatttgcc agttcttgca aatcggcaga aatatcgaag gtatgttccg ccccgcgatg
    34621 cacaccacca attcccccgg tggcaaatac tttaattccg gcaagcgccg caataatcat
    34681 cgttgaagcc acagtggttg cgccattttt tccggcggca acaacaaaag gtaaatcgcg
    34741 acgactaact ttggtcacgt tatgcccttc acgacccagt aattcaattt cttctttgct
    34801 taaacccact ttcatcacgc cgccaataat ggcgatagtg gcaggtacag cgccctgttt
    34861 acgaatagtt tcttcaactt caattgcggt ctgggcattt tgtgggaacg gcatcccgtg
    34921 agaaataatg gtcgattcca gcgccacaac cggttttttg ttttttaaag cgtcctgcac
    34981 ttccggggaa atttgtaata attcagggga aatttttaat tcagacattc tgcgttctcc
    35041 actaacgata taacgttggc aatcgataaa tcggggttat tggtgtattc acaggagagc
    35101 gccattgacg aacatccctg tgcgaaacga acagattcgg caaacggcat tccgtctacc
    35161 caacacgaag caagtcccgc catcatggca tcgcccgctc cggtaacatt aataacattg
    35221 gttttgatcg gcgcagacca gccactttca ccgctgatat cgctgtaata aacgccatcg
    35281 ccgcccatgc tcaataccag tcggttcagg ccatgttgat ggaaccaggc agcaactttt
    35341 gccacatctt cacgccctga cagcgcaatc ccactcaggg tttccgcttc aaggcggttt
    35401 ggcttgagag tgtggatcag atttagacgg tcgcgcactt tgacacattt ccacgcggaa
    35461 acaggatcga caaatacggg tacgttggcg gcattatcca gaatccatgc cagtgcctct
    35521 tcactgatat tacagtccgc gacaatgacc tttgcactct gaataaattc acggtgctgt
    35581 gcaagatatt cagctgtaat agcgttgcta atattcatgt catttatagc aaccagcatt
    35641 tcaccggtat tatcgagtaa tgataaataa ctcgacgtat tttctcccgg cacaatcagg
    35701 catttatcga cataaacgcc agattgattg gtttgcgtta gcagcgattg accataaaaa
    35761 tcactgccta cggcgctcag tagccaggct ttgttaccca gcaacgccag gttttgtgca
    35821 atattgcgcc ctactccacc aggcgtaaat tttattttac ctggatttga atccgcataa
    35881 tttaatgatt catgtgaata tccggcgaca tcaatattcg ccgagcctat aattacgaca
    35941 taatcctttt cgcgcatagc acgtcctctg gtaaaaaatt accaaccctg tcaatccaca
    36001 agaatataga tactgcaaag acggtttaaa catgtgttca tatttaaaca tgtgctcata
    36061 gtatgcatgt tttacgtaaa gtaaattacc gccagagagg caaaatgggc agatttgagc
    36121 aggctcacaa aatgcgatta aatgcctgaa ttacaaaagc gaggaatttg tgacgagatg
    36181 cacagaacag gaacactaaa aaagggcaga aaagaatctg cccttgagga aataacctta
    36241 gtatttgatg aacgtttcag gtttctactg aaagataaaa ccatttaaat tcataaaatt
    36301 acattcacaa accggtcatt ctctttgcgc tcatccaatg atacagggca gtaaatttct
    36361 gccctgtaac acacctttta ttacgcggct ttcgctactg cgtccacttc cggacgtttc
    36421 aggaaggcat aggccaaact cgccaccagc gtaccggcaa taattgctac caggtaaccc
    36481 agtaccggcg taatagcgcc agggatcagc agaacaaaca gaccaccgtg cggtgccatc
    36541 agtttcgcac caatcgccat tgagattgcg ccagtcagcg ccccaccaac gatacaacac
    36601 ggcagcacac gcatcggatc acgagcagca aacggaattg caccttccga aataaagcac
    36661 agaccaagaa ccagagcggc tttgccacct tcctgctgcg ctttgtcgaa tttgcgacgc
    36721 gccaccattg ttgccagacc cattgccagc ggtggcacca tacctgccgc cataatcgcc
    36781 gccatcgggc cataggtttg agtactcagc agacccacac cgaatgcgta cgctgcttta
    36841 tttaccggac cgcccatgtc agtacacatc atgccaccga ggatcgcccc cagcagaacc
    36901 gcattcgcag tccccatggt ctgcagccag tgagtcaacc cttcgagaat gccagcaacc
    36961 ggtttaccga tcaggtagat catcgccaga ccgaccacca gactggaaat tagcggaatg
    37021 atcaggatcg gtttcagcgc ctccatactc tgtggcagtt tcagttgcgt actgattaac
    37081 ttcgcaatgt aaccagccag gaagcccgca ataataccgc caatgaagcc agaaccggtg
    37141 ctgaccgcca gcataccgcc aatcagaccc ggcgtgagac ccggacgatc ggcaatggag
    37201 aaggcaatgt aacctgccag taccggcacc atcagcgcaa aggctgaacc accaccaatc
    37261 tgcatcagcg ccgctgccaa cgtacccggc tctttaaacg cttcgatacc aaaagcaaaa
    37321 gaaagcgcga tacacagacc acctgcaacg accatcggca gcatgtaaga gacgcccgtc
    37381 agcaagtgac ggtatgcgcc tgcactctct ttcttacctt cagaggtcgc cgtttgagct
    37441 ttgcccgccg gttcatacgg cgttgcttca gcaaccgctt tatccagttc ctgcgcggtt
    37501 ttcttcagcg ccagaccggt agaggtacga tacatcggtt taccagcaaa tttcgccaga
    37561 tccacttcga tatctgccgc cacaatcacc agatccgctg cggctacttc ttcgggagtg
    37621 attgcattac ccgcaccaac agaaccacgg gtttcaactt tcacccacca gccacgtttt
    37681 ttcgcttcgg tttcaatggc ttcagccgcc ataaaggtgt gtgctacgcc agtcgggcaa
    37741 gcagtcaccg caactacgcg tttcggaccg ctggcggcaa ccggtgctgt cgcagcgacc
    37801 ggcgcagtgt aaggtttcgc atggcctttg gcttcactca ggaacagctc agggtgcgca
    37861 actgcccggg aaatatcgcc cagccagaca tttttaccgt tcagcgcgct gtcattcggg
    37921 atggaatcac cgagaacaat cgccatttct gcgtcattcg gattgtcgat gatttccagt
    37981 tttgcttttc gcgccgccgc gcccagcagg gtcttcgcca tataggcgcg tgcctgaccg
    38041 agattagcgt caataatcag cagcgttttc attatgcctc tcctgctgtc agttaaaagg
    38101 ttgtaagtcg acgcgcgcca tcattgcggc caactgcgga cgatcggtaa tacccacatt
    38161 gctttgactt accgccaggg ctgcaacagc agtcgccaga cgcagtgtgt gttcactgga
    38221 ttcacgcatt agcaagccat aaatcaggcc accaaccata gaatcccctg cgccaacggt
    38281 gcttacgaca tcgactgacg gtggtttggc gatccattcg ccggaggcat taacccaaag
    38341 cgcgccttcg gcacccagtg aaataacgac atgcgcaata ccttgttcac gcagcgcatg
    38401 cgcagcttca atcacatctt tcatttcagg cagtttacgg cctgcccaga tttccagctc
    38461 gcggcggtta ggtttcacca gccacggtgc cgctttcaaa cctgctacta acgcttcacg
    38521 gctactatca aagataatgc aagggcactg actacgcagg cgagtcatcc agtcggtgaa
    38581 cgcttccggg ctgacgcctg acggtaagct tccgctgaca cagaccatat cgaactgacc
    38641 gagccagctc agagaatcag tcacaaagcg ttcccagtcg gcgggagtga cttcaaaacc
    38701 cgagaagttg aagtcggtca cttcgccgtc tttttccgtc agcttaacgt taattcgggt
    38761 gcgcccctgt acaacctgga aacggttggc aatgcccagc tcgctgaaca gttgctgaaa
    38821 accatcctga ttgtctttac cgaggaagcc gccaacggtg acatcaattc ccagatcttt
    38881 taatactttg gccacgttga tgcctttacc cgccgcatgc agaccggtgg ttttcaccag
    38941 gttcacttcg ccgcgttcaa tttccgggca gaaaccaaca aggtcataag ccggattaag
    39001 ggtgatagta gcaacacgtc tgctcattat gcgccctccc caagaccagc agcgatagcg
    39061 tcgccgattg ctttcagcgc ctgttcagca tctgcaccct gggcggtaaa gcgtaggcga
    39121 tgacctttct taacgccaag tgccacaact ttcatcagac tacgtccgtt tgccggtttg
    39181 ccggtgccat caaggtttgt cacggtaata tcactgttaa attgtttaat ggtgttgacc
    39241 agcatggtac ctggacgagc atgcaggccg tgttcattgc gcaccacaaa ctccgcgctt
    39301 aacacgtcgt cggtcggcgc atcatcgctg gtcagcagcg ccagcaacgt tgccgcatcc
    39361 gctttcagca agcggtcagc tttattgtcg agcaacaaat cagcgagacg cttaagaacc
    39421 gcaatgggct gatcgtcatt catcgccaca ctcaccagca tggctgccgt ttcgccgtcc
    39481 acatcaaaag catttgccgc acggcttacc gcaatcgcgc tacgcaaatt gccttcggcg
    39541 ctatcgctca gccagatgcc ctgtccgagg ttcagcggtt gttcattgat ggctttggtg
    39601 acgaaagtgg cgtcaactgc ccccgcttct ttcagacgcg cagcgttcag cgcctgaaga
    39661 gtcagcagat cgctggcgac gatatccagc gtcagcattt cgttgtcgag cttcagctgc
    39721 tcactctgct tttcgcccat cagcaatgcg cgaagttctt ctgctgttgt tgctgacttc
    39781 agttgttcag caacggaatc atcgctcagg acgtgggtca gctggcgtag caggcccaga
    39841 tgttcatccg agctggcagc aataccgatt gccacgtacg ctacctgacc gtcaccccag
    39901 gtgacgcctt ccgggaactg aaatacctga acgccggttt tcagcacctg atcgcgggtg
    39961 tcggtagtgc cgtgtggaat agcaatacca ttgccgagga acgttgaggt ttgctgttcg
    40021 cgcgccagca tgccattgac gtagccttct gctacattac cggcctgcac cagcgccgca
    40081 gcgacctggc gaatcgcctc ttctttgtct ccggcctttt cgcccggatg gatgtcctgt
    40141 acggataact ggaacatagt tctcctctct tgctgaattg aaacgattca gcctctatga
    40201 gaaaaaaagc gccaacctag cttagggtta aagacaagat cgcgctgaaa cgtttcaaga
    40261 aagcataata cttctgtttc agcacgcaag gaaagccgta aagttatgag caaaagtttg
    40321 atgtgctgca catttttttg tcatttttcc taattgctga tgggaaaatc cgttatcagc
    40381 aatttcattt cagcagcgtc agcagccaat cagcaacagt gaaatggcat tttgattatt
    40441 cgtggcgctt ttgatttcgc tgtctgttta ttttctgaca agcagcgtaa actccgcgtc
    40501 ttcctcttcc agtgatcgac cagcatgcat aactcccccg cagtctccag cgcgaaatcg
    40561 tttgacctga cctcgacggc gtttttaatc gttgcctttc tcaccggtat tgcgggcgct
    40621 ctgcaaaccc cgacactcag tatttttctt accgatgaag tacatgcccg tccggcgatg
    40681 gtgggattct tctttaccgg cagcgctgtc attgggattc tggtgagtca gtttctcgcc
    40741 gggcgctctg ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg cctgttaggc
    40801 gtgctggcct gcaccctttt tgcctggaat cgcaactact ttgttttgct attcgttggc
    40861 gtctttctta gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct tgcccgtgaa
    40921 catgccgaca aaaccggacg tgaggcggtg atgttcagct cttttttacg cgctcaggtt
    40981 tcactggcat gggtcattgg cccaccgctg gcttatgcct tagcgatggg tttcagcttt
    41041 acggtaatgt atctgagcgc agcggtagca tttattgttt gcggcgtgat ggtgtggctg
    41101 tttttaccgt cgatgcaaaa agagcttccg ctggcgaccg gcacggttga agcgccgcgc
    41161 cgtaaccgtc gcgatacgct gctgctgttt gtcatttgta cattgatgtg gggctcgaac
    41221 agtctgtaca tcatcaacat gccgctattt ataatcaacg aactgcatct tcccgagaaa
    41281 ctggcaggcg taatgatggg gaccgccgcc gggctggaaa tcccgaccat gttgattgcc
    41341 ggatattttg ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc cgtgggtggc
    41401 gtctgttttt acgcaggaat gctgatggcg cattcacctg ccattctgtt gggcttgcag
    41461 ctgctaaatg ctatttttat tggcattctg ggcggcatcg ggatgctcta ttttcaggat
    41521 ttgatgcccg gtcaggcagg ttcagccacc acgctctata ccaacacgtc gcgcgtgggc
    41581 tggatcatcg caggatctgt agcgggcatc gtcgccgaga tctggaatta tcacgctgtg
    41641 ttctggtttg cgatggtgat gattatcgcc actctgtttt gcttactgcg gattaaagat
    41701 gtttaaggcg cggtcagcat ttccagatcg ataagccagg tcattgcctg ctgacgcgag
    41761 ttaccgcaca tttcagcgct ggcttgtaag cctgcacaga ctttcggccg cagtggcgag
    41821 gtgaaaattt tacagcgttg ctgttcatca agctgaatgc agggcgtatt ggcgggcttg
    41881 ccatctggca taccgggaat agggctggaa attgaagggg cagtgcaaca cgccccgcaa
    41941 cccggacggc attccatacg ttgttctctt aaggttaacc accggattga tgagcgcgca
    42001 cagtaccacc ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa aggcccgtcg
    42061 cgagtacttt gtcgcgatat ttttgacatt ttcgactaca ggaatttttc gatgccaaga
    42121 gcgaacgaaa ttaaaaaagg tatggtactg aattacaacg gcaaactgct gctggttaag
    42181 gacattgata ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa aatgcgtttt
    42241 tctgatgtcc gtaccgggct gaaagtagaa gagcgtttca aaggagatga tatcgttgac
    42301 accgtgacgc tgacccgccg ttacgttgac ttctcctatg tcgatggcaa cgaatatgtc
    42361 tttatggata aagaagacta taccccgtat accttcacca aagatcagat tgaagaagag
    42421 ttgctgttta tgccagaggg cggcatgccg gacatgcagg tgctgacctg ggacggtcaa
    42481 ctgctggcgc ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac cgcaccaggc
    42541 atcaaaggtg catctgccag cgcccgtaac aaaccggcga cattgagcac tggtctggtg
    42601 attcaggtac cagaatactt aagcccgggc gaaaaaattc gtatccatat cgaagaacgc
    42661 cgttatatgg ggcgtgctga ctaacttcag ccgcatgcag aaaagggata gctcaggctg
    42721 tccctttttt aatttattat accaatcttc tattttgcgc ttcctgaaca ccttacgccc
    42781 gcaattttca ctcgtttatt gatcgccctc acacttcatc gcattaacaa tccagaccaa
    42841 tttcaattgc tgtcatataa ctttacactg tcattgttaa ttaatcgtta ctaagacgtg
    42901 actcctatga atacaatcgc ctccgttacg ctcccgcatc atgtacacgc tccacgctac
    42961 gatcgccagc agttgcaatc acgtatcgtt cattttggct ttggagcctt tcaccgcgca
    43021 catcaggcgt tactgaccga tcgtgtgctg aatgcccaga gcggcgactg ggggatctgt
    43081 gaaatcagct tgttcagcgg tgatcaactg atgagccagc tccgcgcaca gaaccattta
    43141 tacaccgtgc tggagaaagg ggcggacggc aatcaggcga taattgtcgg tgccgttcac
    43201 gaatgcctga atgcaaaact ggattcctta gcggcaatta ttgagaaatt ttgcgagcca
    43261 caggtggcaa ttgtttccct gacgattacc gaaaaaggct attgtattga cccggccacc
    43321 ggtgcactcg acaccagcaa tccgcggatt attcacgatc tacaaacccc tgaagaacct
    43381 cactccgcac cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg cggccttaca
    43441 ccgtttaccg tgctctcctg cgacaatatt cccgacaatg gccatgtggt gaaaaacgcg
    43501 gtgctgggaa tggcagaaaa acgttcgcca gaactcgccg ggtggataaa agaacacgtc
    43561 agttttccgg gaaccatggt cgaccgcatt gttccggctg caaccgacga atcactggca
    43621 gaaatcagcc agcatctggg ggtaaatgat ccctgcgcga ttagctgcga accgtttatc
    43681 cagtgggtgg tggaagataa cttcgtcgct gggcgtcctg cctgggaagt cgcaggtgta
    43741 caaatggtga atgatgtcct gccatgggaa gagatgaaac tgcgtatgct taatggcagc
    43801 cactcttttc tcgcttatct gggttacctc tcaggattcg cccatatcag tgattgcatg
    43861 caggatcgcg catttcgcca tgctgccaga acattaatgc tggatgagca agcgccgaca
    43921 ctgcggatta aagatgtcga tttaacacaa tatgcggata agttaattgc acgttttgct
    43981 aatccggcgc tgaaacataa gacctggcaa atcgcgatgg atggcagcca gaaattaccg
    44041 caacgcatgc tggcaggtat tcgcatacat ctggggcgcg aaacggactg gtcgttgctg
    44101 gcattaggcg ttgcaggctg gatgcgttac gtcagcggcg ttgatgatgc cggaaatgcc
    44161 attgatgttc gcgatccgct tagcgataaa attcgcgatc ttgttgcggg cagcagcagt
    44221 gagcaacgcg tcaccgccct gctttccctg cgtgaagttt tcggtgatga tctgccagat
    44281 aacccgcatt ttgtgcaggc catcgaacaa gcctggcaac aaatcgcaca attcggcgcg
    44341 catcaggcgc tattaaacac cctcaaaatt taacgatttc tgcggttaaa gcggatgaag
    44401 ctcgccttcg tccgctctcc ccttctcttt tctgccgttt ttagccagga ttaacgctca
    44461 gttaacttac cagagtattg cggagccgtt gtgaccagga ccaacctcat caccggtttt
    44521 ctcggcagcg ggaaaaccac gtcgattctt catctgttag cccataaaga tcccaacgaa
    44581 aaatgggcgg tactggttaa tgaatttggg gaagtcggaa ttgatggtgc tttgctcgcc
    44641 gatagcggcg cactgctgaa agagatcccc ggcggctgca tgtgctgcgt taatggttta
    44701 cccatgcagg tagggttgaa taccttactg cgtcagggaa aaccagaccg cttgttgata
    44761 gagccgaccg ggctgggcca tccgaaacag atcctcgatc tcttaaccgc accagtctat
    44821 gaaccgtgga tagatctacg cgccacgttg tgcattctcg atccacgcct gctgctggac
    44881 gaaaaaagcg ccagcaatga aaacttccgt gaccagctgg ctgccgcaga catcattgtc
    44941 gccaataaat ccgaccgtgc gacacctgaa agcgagcaag cgctacagcg ctggtggcag
    45001 caaaatggtg gcgatcggca attaattcac agtgagcatg ggaaagttga cggtcatctt
    45061 ctggatttgc cgcgtcgcaa tttagccgag ttgcccgcca gcgccgcgca ttctcatcag
    45121 catgtcgtga aaaaagggtt agcagcgtta agcctgccag agcatcaacg ctggcgtcgc
    45181 agtctgaaca gtggacaagg acatcaggcc tgcggctgga tattcgacgc tgatacggta
    45241 ttcgacacca ttggcattct ggaatgggcg cgacttgcac cggtggaacg cgtcaaaggc
    45301 gtgctgcgta ttcccgaagg gctggtgcga atcaaccgtc agggcgatga cctgcacatt
    45361 gaaacgcaaa acgttgcgcc accggacagc cgtattgagc tgatttccag cagcgaagct
    45421 gactggaatg gcttgcagag cgcgctgttg aagcttcgtt tagcgactac cgcgtaaggt
    45481 tgcctgcgtt tttcagtaag ataattagag aaaatatgat taaaaatttg ccgcaaatag
    45541 tgttgttgaa tattgtcggc ctagcgctgt ttctttcctg gtatattccc gttaatcatg
    45601 gattctggtt gccgattgat gcggatattt tttatttctt taatcagaaa ctggtcgaaa
    45661 gtaaggcctt tttgtggctg gttgcattga ccaacaatcg cgctttcgac ggttgttcac
    45721 tgctggcgat gggaatgttg atgctgagtt tctggctgaa agaaaacgcc ccaggcagac
    45781 gacgtatcgt gattattggt ctggtcatgc tattaactgc agtggtatta aatcagctgg
    45841 gtcaggcatt aattcctgta aaacgggcca gcccaacatt gacttttacc gatattaacc
    45901 gcgtcagcga actgctctct gtccccacga aagatgcctc acgagatagc tttcccggcg
    45961 atcacggcat gatgctgctt attttttcgg cattcatgtg gcgttatttc ggcaaagttg
    46021 caggtcttat cgcccttatt atttttgtgg tttttgcgtt tcccagagta atgattggcg
    46081 cacactggtt ttctgacatc attgtcggtt cgatgaccgt gatattgatc ggtttgccct
    46141 gggtgttgct gacgccatta agtgatcgat taatcacctt ttttgacaaa tcactaccgg
    46201 gaaaaaacaa acatttccaa aacaaataac tcacagtaat taacatcatc aggtttattt
    46261 ttatagtgag gataatcctg atgatgcgca ccgtgctttc atccatcgaa agcaaaaatc
    46321 attctctaag taaatgaatg gattgcatgc gtttcactca atcgtacttt aattgaccaa
    46381 tcccgcttat taactttctg tatcactttt tcttataaaa aatcatgtaa aaccgctcgc
    46441 caagaccgca ccaatcgggt aatctcgaac tcgttttgcc tcggcggtag attatcctca
    46501 cagcatataa ttttgtgcgt tagtccacag atttggcctt aaggaattgt ttcaacatgc
    46561 ccaggtaatt agtctcgtgt cgcttggcat ttttttataa cgatatttgt cgttaaggac
    46621 ttcaagggaa aacaaacaac atggtcaaat ctcaaccgat tttgagatat atcttgcgcg
    46681 ggatccccgc gattgcagta gcggttctgc tttctgcatg tagtgcaaat aacaccgcaa
    46741 agaatatgca tcctgagaca cgtgcagtgg gtagtgaaac atcatcactg caagcttctc
    46801 aggatgaatt tgaaaacctg gttcgtaatg tcgacgtaaa atcgcgaatt atggatcagt
    46861 atgctgactg gaaaggcgta cgttatcgtc tgggcggcag cactaaaaaa ggtatcgatt
    46921 gttctggttt cgtacagcgt acattccgtg agcaatttgg cttagaactt ccgcgttcga
    46981 cttacgaaca gcaggaaatg ggtaaatctg tttcccgcag taatttgcgt acgggtgatt
    47041 tagttctgtt ccgtgccggt tcaacgggac gccatgtcgg tatttatatc ggcaacaatc
    47101 agtttgtcca tgcttccacc agcagtggtg ttattatttc cagcatgaat gaaccgtact
    47161 ggaagaagcg ttacaacgaa gcacgccggg ttctcagccg cagctaataa accgtttgga
    47221 tgcaatccct tggctatcct gacgagttaa ctgaaagcac tgcttaggca gtgctttttt
    47281 gttttcattc atcagagaaa atgatgtttc cgcgtcttga tccaggctat agtccggtca
    47341 ttgttatctt ttaaatgttg tcgtaatttc aggaaattaa cggaatcatg ttcatacgcg
    47401 ctcccaattc tggacgtaag ctcctgctta cctgcattgt tgcaggcgtg atgattgcga
    47461 tactggtgag ctgccttcag tttttggtgg cctggcataa gcacgaagtc aaatacgaca
    47521 cactgattac cgacgtacaa aagtatctcg atacctattt tgccgacctg aaatccacta
    47581 ctgaccggct tcagccgctg accttagata cctgccagca ggctaacccc gaactgaccg
    47641 cccgcgcggc gtttagcatg aatgtccgta cgtttgtgct ggtgaaagat aaaaaaacat
    47701 tctgttcatc tgcgaccggt gagatggaca ttccactcaa tgaattgatt ccggcgctcg
    47761 acattaataa aaatgtcgat atggcgatct tacccggcac gccgatggtg ccgaacaaac
    47821 ccgcaatcgt catctggtat cgcaaccctt tgctgaaaaa tagcggcgtc tttgccgctc
    47881 tgaatctcaa cctgacgcct tcactctttt atagttcacg gcaggaagat tacgatggcg
    47941 tcgccctcat tattggcaat actgcgctat ctaccttttc ttcacgtttg atgaacgtta
    48001 acgaattaac cgacatgcca gtccgtgaaa ctaaaattgc gggcattcct ctgaccgttc
    48061 ggctttatgc agatgactgg acatggaacg atgtgtggta cgcattttta ctgggcggca
    48121 tgagtggaac tgtcgttggc ctgctctgct attacctgat gagcgtacgt atgcgccccg
    48181 gcagagaaat catgaccgcc atcaagcgcg aacaatttta cgtggcgtat caaccggtgg
    48241 tggatacaca agctttgcga gtaacgggcc tggaagtact gctacgctgg cggcatcctg
    48301 tcgcgggaga aattcccccg gatgccttca ttaactttgc cgaatcgcaa aagatgattg
    48361 tgccgctgac tcagcacctg tttgagttaa ttgcccgcga tgccgcagaa ttagaaaaag
    48421 ttctgccggt aggcgtcaaa tttggcatta acattgcgcc ggaccatctg cacagcgaaa
    48481 gctttaaagc agatatccag aaactgctca cttccctacc cgcacaccat ttccagattg
    48541 tgctggaaat taccgagcgc gatatgctga aagagcaaga agccacacaa ctcttcgcct
    48601 ggctgcactc ggtcggcata gaaattgcta ttgatgactt cggcaccggg cacagcgcgc
    48661 ttatctatct tgagcgtttt acgctcgatt atctgaaaat tgaccgtgga tttatcaacg
    48721 ccatcggtac ggaaacgatc acttcccccg tacttgacgc ggtgctgacg ctggcgaaac
    48781 gcctcaatat gctgacggtt gctgaagggg tcgaaacgcc ggaacaggcg cgatggctaa
    48841 gcgaacgcgg cgttaatttc atgcaaggct actggattag tcgcccgtta ccgctggacg
    48901 attttgtccg ctggctgaag aaaccgtata cgccgcagtg gtaaggtgtg cttacgtccc
    48961 ttattattca tagtgaaagc atgccggatt gcggctaatg atgagtaaaa ggaaatccgt
    49021 tgcagatgat tgtgcgcata ctgctgctgt ttatcgctct gttcaccttt ggtgcgcagg
    49081 cgcaggctat caaggaaagc tatgcctttg ccgtgctggg cgaaccccgg tacgcgttta
    49141 atttcaacca ttttgattat gtgaaccccg ccgcgccaaa aggtgggcag ataacgttgt
    49201 cagccctcgg taccttcgat aatttcaacc gctatgcact gcgcggcaac ccgggcgcac
    49261 gcaccgagca gctgtacgac acgctattta cgacttccga tgacgaacca ggcagttatt
    49321 acccgctgat tgctgaaagc gcacgctatg ctgacgatta ttcctgggtg gaggtcgcta
    49381 ttaatccgcg cgcccgtttt catgatggtt ctcccattac tgcccgcgat gtagagttta
    49441 cttttcaaaa atttatgacc gaaggcgtgc cgcaatttcg tctggtctac aaaggcacca
    49501 ccgtcaaagc cattgcgccg ttaaccgtgc gcattgagtt agctaaaccc ggcaaagaag
    49561 atatgttgag tctgttttcg ctgccggtat ttccagaaaa gtactggaag gatcacaaac
    49621 ttagcgatcc gctcgccacg cctccgcttg ccagtggtcc gtaccgaatt acgtcctgga
    49681 aaatggggca aaatattgtc tattcccgcg tgaaagatta ctgggcagca aacttaccgg
    49741 taaaccgtgg acgctggaat ttcgacacca ttcgctacga ttattacctc gatgataatg
    49801 tcgcctttga agcgtttaaa gcaggtgctt ttgatttgcg tatggaaaac gacgctaaaa
    49861 actgggccac gcgttatacc ggtaaaaatt tcgataaaaa atacatcatt aaagatgagc
    49921 aaaagaacga atcagcccag gatacgcgct ggctggcgtt taatatccaa cgtccggtat
    49981 tcagcgatcg ccgggtccgc gaagcgatca ccctcgcctt tgactttgaa tggatgaaca
    50041 aggcgttgtt ttacaatgcc tggagtcgca cgaacagtta ttttcagaat accgaatacg
    50101 cggccagaaa ttaccccgac gccgcggagc tggtgcttct ggcaccaatg aaaaaagatc
    50161 taccgccaga agtcttcaca caaatctacc agccgccggt atccaaaggc gatggctacg
    50221 atcgtgacaa cctgttaaaa gccgacaaac ttctcaacga agcgggctgg gtgctgaagg
    50281 gtcagcaacg cgttaatgcc acaacgggtc agccactcag ctttgaatta ttgcttcctg
    50341 caagcagcaa tagtcagtgg gtattgccgt tccagcacag cctgcaacgg ctgggtatca
    50401 acatggacat tcgcaaggtg gataactcgc aaatcaccaa ccgcatgcgc agtcgcgact
    50461 atgacatgat gccgcgcgta tggcgggcga tgccgtggcc cagttccgat ttacagattt
    50521 cctggtcatc ggaatatatc aattccactt ataatgcccc cggcgtgcaa agcccggtta
    50581 tcgactcgct gatcaaccaa attattgccg cgcagggaaa taaagaaaaa ttactgccgt
    50641 tggggcgagc actggatcgc gtattaacgt ggaattatta catgctgcca atgtggtaca
    50701 tggcggaaga ccgtctcgcc tggtgggata aattctccca acccgctgta cgccctgttt
    50761 acagcctggg tatcgatacc tggtggtatg acgttaataa agcggccaaa ctgccgtcag
    50821 ccaagcaaca gggagagtag atgggcgctt atctgattcg ccgtctgttg ctggtgatcc
    50881 caacgttatg ggcgattatc actatcaact ttttcatcgt gcaaattgcg cctggcggtc
    50941 cggtcgacca ggccatcgcc gccattgagt ttggtaatgc cggagtatta cccggcgcag
    51001 gcggtgaagg cgttcgcgcc agccatgcgc aaacgggcgt cggtaatatc agcgacagta
    51061 attaccgtgg cggacgcgga ttagatccag aagtgatcgc cgagatcact catcgctacg
    51121 gtttcgataa gccgctccac gaacgttact tcaaaatgct ctgggactac atccgctttg
    51181 attttggtga tagcctgttt cgcagcgcct cggtgctgac gctgattaaa gacagtctgc
    51241 cggtttccat caccctcgga ttatggagca cgctgattat ctatctggtg tcgattccgt
    51301 taggcattcg caaagctgtt tataatggga gccgctttga cgtctggagt agcgcattta
    51361 tcatcatcgg ctacgccatt ccggcctttt tgtttgccat cctgctgatt gtcttcttcg
    51421 caggcggcag ctatttcgac ctgttccctc tacgcggcct ggtttccgct aactttgatt
    51481 cgctgccgtg gtatcagaaa atcaccgatt atctgtggca tatcacgctg ccggtgctgg
    51541 cgacggtgat tggtggcttt gcggcgctga ctatgctgac aaaaaactca ttccttgatg
    51601 aagtgcgcaa gcaatacgtg gtgaccgcgc gtgcgaaagg ggtaagtgaa aaaaatattc
    51661 tctggaaaca tgtgttccgc aacgccatgc tgctggtgat tgccggtttt ccggcgacgt
    51721 ttatcagcat gttttttacc ggctcgctgc tgattgaggt gatgttttca ctcaatggtc
    51781 ttggcttact gggctacgaa gcgaccgtct cgcgcgatta tcctgtaatg tttggtacct
    51841 tgtatatttt caccctgatt ggcctgctgc tgaatattgt cagtgatatc agctatacgc
    51901 tagtcgatcc gcgtatagat tttgagggac gttaatgtcg cgactcagcc ccgtcaatca
    51961 ggcccgttgg gcgcgttttc gccataaccg tcgcggctac tggtcgttat ggattttcct
    52021 cgttttgttt ggtttgagtt tgtgttctga acttatagcc aacgataaac cgttgctggt
    52081 gcgttatgac ggcagttggt atttcccgtt gttgaaaaat tacagtgaaa gcgattttgg
    52141 cggcccgctg gcaagtcagg ctgattatca ggacccgtgg ctgaaacaac ggctggaaaa
    52201 taatggctgg gtactgtggg caccgattcg ctttggtgct accagtatca actttgctac
    52261 cgataagccc ttcccttctc ccccctcccg gcaaaactgg ctgggaacgg atgccaacgg
    52321 cggcgatgtg ctggcacgta ttctctatgg cacgcggatc tcggttctgt ttggcctgat
    52381 gctgactctc tgttccagcg tgatgggcgt gctggcgggg gcgctacaag gctattacgg
    52441 cggtaaagtc gatctctggg ggcaacgctt tattgaagta tggtcgggga tgccgacgct
    52501 gtttttgatt attttacttt ccagcgtcgt acagcctaac ttctggtggc tgctggcaat
    52561 tactgtcttg tttggctgga tgagtctggt cggcgtggtg cgggcggagt ttttacgtac
    52621 ccgtaatttc gactacattc gtgcggcgca ggcgcttggc gtcagcgatc gcagtatcat
    52681 cctgcgtcat atgttgccta atgccatggt cgcgaccctc acctttttac cgtttatttt
    52741 atgtagttcg atcaccaccc tgacctcact cgatttcctc ggcttcggtc tgccgctcgg
    52801 ttcaccgtca ctcggtgaac tgctgttaca agggaaaaat aaccttcagg ctccgtggct
    52861 tgggatcacc gccttcttgt cggtggcgat attattgtct ttgctgatct ttattggtga
    52921 agccgtccgc gacgcatttg atcctaataa ggcggtgtag catgacgcaa actctgttag
    52981 cgattgaaga tttgtcggtg ggttttcgcc atcagcaaac cgtacgtaca gtagtcaatg
    53041 atgtttcact acagattgag gctggcgaaa cgctggcgct ggtgggtgag tcaggttcag
    53101 gcaaaagcgt taccgcgctg tcaattttac gcctgctccc atccccgccg gttgaatatc
    53161 tctccggcga tattcgtttt catggcgaat cgctgcttca tgccagcgat caaacgttgc
    53221 gcggtgtacg cggtaataag atcgccatga tttttcagga gccgatggtg tcgttaaatc
    53281 cattgcatac cctggaaaaa cagctttatg aagtgctttc actccaccgc gggatgcgtc
    53341 gggaagcggc tcgtggcgaa attcttaact gccttgatcg cgttggtatc cgccaggcgg
    53401 caaaacggct gacagattat ccgcatcagc tctccggcgg cgaacgccag cgggtgatga
    53461 ttgcgatggc gctgttaacg cgaccggaat tattaattgc cgatgaacca accaccgcac
    53521 tggacgtctc tgtccaggcg cagattttac agctgttgcg cgaactgcaa ggcgagctga
    53581 atatgggcat gctgtttatt actcataacc tcagcattgt cagaaaactg gcccaccgcg
    53641 tggcggtaat gcaaaacggt cgctgtgtcg agcaaaataa cgccgctacg ctatttgcct
    53701 cacccactca tccttacaca caaaagctac tcaacagtga accgtcaggc gatccagtgc
    53761 cgttgccaga acctgcctca acgttgctgg atgttgaaca gcttcaggtt gccttcccca
    53821 ttcgcaaagg gattttgaag cgcattgtgg atcataatgt ggtggtgaaa aacatcagtt
    53881 ttacgctacg ggcgggtgaa acgctgggtt tggtaggcga gtctggttcc gggaaaagca
    53941 cgacgggact ggcgctgctg cgactgatta attctcaggg cagcatcatc tttgacggtc
    54001 agccactgca aaatttaaat cgccgccagc tgttacctat tcgtcatcgc attcaggtgg
    54061 tatttcagga tccaaactcc tcgctcaacc cacgactcaa cgttttgcag attattgagg
    54121 aaggcttacg ggttcaccag ccgacgcttt ctgccgcaca acgcgaacaa caagtgatag
    54181 ccgtgatgca tgaagtggga ttagatcctg aaacacgcca ccgttatccg gcggagttct
    54241 ctggtggtca gcgacaacgt attgcgattg ccagggcatt aattcttaag ccctcgctga
    54301 tcatacttga tgaaccgaca tcatcactcg acaaaacggt acaggcgcaa atattgacgc
    54361 tattgaaatc attgcaacaa aagcatcaac tggcctattt gtttatcagc catgatttgc
    54421 acgttgtccg cgcgttatgt catcaggtta tcgtactgcg acaaggggaa gtagtggaac
    54481 aaggaccgtg cgcgcgcgtg tttgccacac cgcagcagga gtatacgcgt cagctactgg
    54541 cgttgagctg acgcttaaaa aggattgtag tttgaaaagg gttcggcaat tgccacacca
    54601 aaatttttca atcggcatgt tgcagcaaac tcgtcctggc gatttacaaa caggcacggc
    54661 tcaccttcac actccagcac cgaacatgga acttcgatgt cgcttaacgc ctggcttagt
    54721 ttatacatca ccgaccatgc attatcacca tccgggctaa gcaatttaag cgccaccagg
    54781 ctgttgtcac cgcacggtcc attttgcgga atcggttcaa ccttcgaacc tgcgaaacca
    54841 gcagaccagc gatagttctg cggcagtcga tggacgattg agagttgtaa tgaagtcact
    54901 tattttcccc ggaagcacat ttacttcaca atttattcac atcaatttta acacatcatc
    54961 aacaatccgt ctttatacag atcgtaaaga ttgatgcctc gcctgcgtgg cctctatggc
    55021 tctgatttaa gtaatatctg cgggcgtggt cacgtttgcg gggctatatg tacccgtttc
    55081 tgcgatctaa ctcaaccttt ttaactacaa tgatgtgact ttttacataa attgatttta
    55141 cataaaataa acatatatcg ggggaatgat agatttgtgg ttgacatgca tcaacaaagg
    55201 aagcctttta gcttcctcgt tgtgcaatag atcaccgttt tttcggccga ctggcgtaca
    55261 gacagaagag aatggagctg gttgcgcaga atgcaattga ccaaatcatc ggccatgcag
    55321 agttaaaggt cgcaagagaa agcaatgcgc caacaattgc ccctatgcca aaacggaagg
    55381 ttcctgccag cgaagatgcc gttcccgcca tatggggaaa ctcatcaaga atgaccgcca
    55441 tcgcattgga tgacaccatc gacacgcagc ccacaaacgc cgcaacgcca accaccagcg
    55501 accagaatcc cagccccagt agcgcactga tgaccatcca cgctgccata ataaactgta
    55561 tccacaaccc cgagcggaac atatttaacg cgccaatacg gcggacgaag cggctattaa
    55621 agatggtcat cacgaacagg aaaacaatgt ttagtgcaaa gtaataacca aagttttccg
    55681 gcgcgacgtg gttaatttca atgtagacaa acggtccggc gcttaagaac gagaacatcc
    55741 cggcaaagct gaaaccactg gccagcatat aactcaggac acgcttatga cggaacagcg
    55801 ccgcaaagtt accaatagtg gtacgaatgt gaaaaggctg gcgacgctcc ggtggtaagg
    55861 tttctttgat cagaaagaag atcattgccg aagccagaat cgccgccacg gcgaggatcc
    55921 agaagatgta atgccagctc agccacacca gcacccagcc gccaactatc ggtgccatca
    55981 gcggtgcaat ggttgtcacc agcatgacaa acgacatcat ccgcgagaac tcttctttag
    56041 ggtagatatc acgcatcaaa gcgttgataa ccacgctggc cgcagccgcc gccagcccgt
    56101 ggaagaaacg catcacaatc agctgatcga tggtttgtgc caacgcacac gccaccgcgg
    56161 cggcggcaaa caccagcgta ccgccaagaa ccaccggctt acgcccgaag ctgtctgcca
    56221 tcggcccgta gattaactgc cccaacgcaa agcccagaat ataagtactg agggtcatct
    56281 gcgtactgcc cgccggtacg ccaaactgcg ctgaaattac cggtagcgcg ggcagataca
    56341 tatcaatcga cagcggcatc aacatggcca gcaggccaag gataaaaaca atagcaaacg
    56401 acgaatgctg tcgggtggtc acaacgggct cctgaaagtc attgaaaagt tagacgacgc
    56461 tggcaatttc ttcttcagtt aacggacgat attcaccggg ggctaaatca gcatccagcg
    56521 taatgccgcc aatacgttca cgatgcagct caaccacatg gttacccacg gcggcgaaca
    56581 tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag acgaacctgc gttggggtaa
    56641 tcacttccag caccgccggc ttagtgagat ctttttcgtt atgcagctgc acgcctttag
    56701 caaattgctc tgccgtatcg tcagctacgg gcgattccag cgtcaccaga taggtcttct
    56761 cgcaatgatg gcgcggagaa gtaatgcggt gcgaccactg accatcatct gtcattagta
    56821 ccagaccggt ggtatcgata tccagccgcc ctgcggcgtg cagtttccac gccactggtt
    56881 catccagaaa atagagcacc gttgggtgat cagggtcgtc cgtggagcaa acatagccct
    56941 gaggcttatt gagcatgaag taacgtggac cgtgttgctg cgccagcggg ttgccatcat
    57001 aagcgacatc atgttcagga agcagtttga acgctgcatt acggacgatt tcgccatcga
    57061 cggtgacacg attgccgcgg atttcacgcc cggcaatagc acggctaacg ccgagttgct
    57121 gtgcgataaa tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaacg gaggtcgggc
    57181 attgacccga aaatctgaac tgttgtctgc ccagtatagc ggtctaataa cttccctcaa
    57241 gggaaaaaga accgtggcat actattagca gaataatcta cctacgcgag accatgattt
    57301 ttacacttcg cccatatcag caagaagccg tggatgccac gctcaaccat tttcgtcgtc
    57361 ataaaacccc tgccgttatc gtgctgccca ccggtgcagg taaaagcctg gtgatagcgg
    57421 aacttgcgcg gctggctcgt ggtcgcgtac tggtgctggc acacgttaaa gaactggtgg
    57481 cgcaaaacca tgcaaagtat caggcgctgg ggctggaagc cgatattttt gccgccgggc
    57541 taaaacgcaa ggagagccac ggtaaagtgg tatttggcag cgtgcagtcg gtcgcccgta
    57601 atcttgatgc ctttcagggt gaattttcgc tgttgattgt cgatgaatgt caccgtattg
    57661 gtgatgacga agagagccag tatcagcaaa tcctcactca cctgacaaaa gtgaatcccc
    57721 acttacgcct gctggggctg actgccacgc cttttcgatt gggcaaaggc tggatctacc
    57781 agttccatta tcacggcatg gtacgcggcg atgagaaagc ccttttccgt gactgcattt
    57841 atgagctgcc gctgcgttat atgattaaac acggctatct gacgccgcca gaacgactgg
    57901 atatgccagt agtgcaatac gatttcagcc gcttgcaggc acagagtaac gggctgttca
    57961 gcgaggccga tctcaaccgc gagctaaaaa aacaacaacg tattaccccg cacatcatca
    58021 gccagattat ggagtttgcc gaaaagcgta aaggagtgat gattttcgcc gcgacggtcg
    58081 aacacgcaaa agagattgtg ggattactac ccgccgaaga tgctgcactg attactggcg
    58141 acacccccgg cgctgagcgc gatgtgttaa ttgaagattt taaagcccag cgttttcgct
    58201 atctggtcaa cgtcgcagta ctgaccaccg gatttgacgc cccgcacgtc gatcttatcg
    58261 ccattctgcg ccctaccgaa tcagtgagtc tttaccaaca aattgtcggg cgcggtctgc
    58321 gtctcgctcc gggcaagact gattgtttaa ttcttgatta tgcgggtaat cctcacgatc
    58381 tctacgcgcc ggaagttggt acaccgaaag gcaaaagtga caacgttccg gtacaggttt
    58441 tctgccctgc ctgcggtttt gccaacacct tttgggggaa aacgaccgcc gacgggacat
    58501 tgattgaaca ctttggtcgc cgctgtcagg gatggtttga agatgacgac ggtcatcgcg
    58561 aacagtgtga cttccgtttc cgttttaaaa attgcccgca atgtaacgcg gaaaacgata
    58621 ttgccgcccg ccgctgccgc gaatgtgaca ccgtactggt tgatccagat gatatgttaa
    58681 aagcggagct acgactgaaa gacgcgctgg tattacgctg tagcggcatg tctttacagc
    58741 atgggcacga cgagaaaggc gaatggttga aaatcaccta ttacgatgaa gacggcgcgg
    58801 atgtgagtga gcgtttccgt ctgcaaacgc ccgcccagcg aactgccttc gagcagcttt
    58861 ttatccgccc gcatacgcgc acaccgggca tccctctgcg ctggatcacc gccgccgata
    58921 tcctcgccca gcaagcctta ttgcgacacc cggattttgt cgttgcccgc atgaaaggtc
    58981 agtactggca agtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt cgggcgcacg
    59041 aattacgcgg ttaatggtag ttttgattga tgtataaggc gattgagtat agaatctcgc
    59101 ccgcttttgc atacgcaaag cagatcactt acctgttgct gggtcgcctg tagcaggatt
    59161 aatttaagag agaaagaaat gtttactatc aacgcagaag tacgtaaaga gcagggtaag
    59221 ggtgcgagcc gccgcctgcg tgccgctaac aagttcccgg caatcatcta cggtggcaaa
    59281 gaagcaccgc tggctatcga gctggatcac gacaaagtca tgaacatgca agctaaagct
    59341 gaattctaca gcgaagttct gaccatcgtt gttgacggta aagaaatcaa agttaaagct
    59401 caggacgtac agcgtcaccc gtacaaaccg aagctgcagc acatcgactt cgttcgcgct
    59461 taattgctga ataagttgta aaaaaccccg ctccggcggg gttttttgta tctgcagatt
    59521 atgcctgatt acgatattgc tattttttgc aggccagata aggcgttcac gccgcatctg
    59581 gcattcataa cacaaaaccg attaattgcc gccggacgtc cggcgctgca attgatcgcg
    59641 caaattcggc ggcgtgcctt taatagtcag ggtatcggtc gccggatccc agaaaatccg
    59701 ttcgcccaac agcatcgcat caaaattaat agtcaagcca ccgccgctac cggcaaactt
    59761 cgtcaactga cgcagtgtgc tacggtctgc cgggaagctc tcttccagtt cgtagccttt
    59821 ttcagccgca aactccgtga agctcacttc gctgacgcct gccagctctt tcgacagcga
    59881 ttccagttcg atctcttcgc ctgcctgcag ctgctcatta cagtagctat aaacctgctg
    59941 gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat catcaaccgc
    60001 ttgcaacagc ccacggtttt gcgctttcgc gttcaatcct tcgctggccc cgaggaaatc
    60061 cataaagaaa tccgccactt tgcgccctac tctccctttc aggaaagtga gatagcgggt
    60121 cgactctgga ttggtttccc attcggttaa atcgatacgc gcaacaatat ccgcatgatt
    60181 gatatcaaga taatgggttg ggttgatgtc gagattttcg ttaacgcgca tactgctcag
    60241 gttgctcaga actgccacca gcaaatactc caccgccaga tagcgatagt ggcaaaacag
    60301 cacgaatcca ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc gaccggttgc
    60361 cgcgcggcta aatgccagaa agtcctcttc accctgacgc tgtaagcgca gcgtctgcgc
    60421 cagttcactc tcttcgctaa acaagccgta agctttattt ttggcgctat agacccgatg
    60481 cagttctgct accatctcaa cgacggtttc tgtcggttcc agcaatgaat cgcgcaacac
    60541 cagctcaagg ttctgctcat cacgcttgat aagctggtgc agagcaatct ggttgatatc
    60601 cagactcatg ataaactctc ctttaagacc gggcggtatt caaccaccgc cggttaagcg
    60661 acgcaataaa agatggcggt cattttacag aaggcggcgt aacgagaatg atttaacgca
    60721 taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat caactaactg
    60781 atttcgattt atgccacaaa tttcccgcta cagtgatgaa caggttgaac aattgctcgc
    60841 agagctgctc aacgtactgg aaaaacataa ggctccgacc gacctttccc tgatggtgtt
    60901 aggcaacatg gtcactaacc ttatcaacac cagcattgcc ccggcccaac gccaggcaat
    60961 tgccaactct tttgcccgcg ccttacagtc ctctatcaac gaagacaaag cgcactaagg
    61021 gaaacagata acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa gtctcccaga
    61081 tggtcagttg ggggcactgg tttgcactgt tcaatattct gctttcgctc gtcattggca
    61141 gccgttacct gtttatcgcc gactggccga ccacgcttgc tggtcgcatt tattcctacg
    61201 taagcattat cggccatttc agcttcctgg tgttcgccac ctacttgctg atcctcttcc
    61261 cgctgacctt tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc attctggcaa
    61321 cggcgggaat gacgctatta ctgatcgata gcgaagtctt tactcgtttc catctccatc
    61381 ttaatcccat cgtctggcaa ctggttatca acccagacga aaatgagatg gcgcgcgact
    61441 ggcagctgat gttcatcagc gtgccggtta ttttattgct tgaactggtg tttgcgacgt
    61501 ggagctggca aaagctgcgc agcctgacgc gtcgtcggcg cttcgcgcgc ccgctggccg
    61561 cattcttatt tatcaccttt atcgcctcgc atgtggtgta tatctgggcc gatgccaact
    61621 tctatcgccc tatcaccatg cagcgcgcta acctgccgct ttcgtacccg atgacggcgc
    61681 gacgttttct tgagaagcat ggcctgcttg atgcgcagga gtatcaacgc cgtcttattg
    61741 agcaaggtaa tccagacgcc gtttccgttc agtatccgtt aagcgaactg cgctatcgcg
    61801 atatgggcac tgggcagaat gtgctgttga ttactgtcga tggcctgaac tactcacgct
    61861 tcgagaagca gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg ttcacgcgcc
    61921 atatgagctc cggcaacact acagacaacg gcatctttgg cctgttctat ggcatctcgc
    61981 cgagctatat ggacggcatt ctgtcgaccc gtacgcctgc ggcattaatt actgcgctta
    62041 atcagcaagg ctatcagctg gggttattct catcagatgg ctttaccagc ccgctgtatc
    62101 gccaggcatt gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc gacgagcaga
    62161 ccgccacgca gtggatcaac tggctgggcc gctacgcaca agaagataac cgctggttct
    62221 cgtgggtctc tttcaatggc actaacattg acgacagcaa tcagcaggca tttgcacgga
    62281 aatatagccg ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc aatgcactgc
    62341 gtgattctgg caaactggac aacacggtgg tgattatcac tgccggtcgg ggtattccac
    62401 tgagcgaaga ggaagaaacc tttgactggt cccacggtca tctgcaggtg ccattagtga
    62461 ttcactggcc aggcacgccg gcgcagcgta ttaatgcgct gactgatcat accgatctga
    62521 tgacgacgct gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa tattcgcaag
    62581 gtcaggattt gttcaaccct caacgccgtc attactgggt taccgcagcg gataacgata
    62641 cgctggcaat taccaccccg aaaaagacgc tggtgctgaa caataacggt aaataccgca
    62701 cttacaactt acgtggtgaa agagtgaaag atgaaaaacc acagttaagt ttgttattgc
    62761 aagtactgac agacgagaag cgttttatcg ctaactgatt aattataaat cagttagcga
    62821 aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta acagccacga gtcggcacgt
    62881 agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca aatcctctcg
    62941 tgccgaccaa aaatcccaaa aaaaaccaac ccttacggtt ggttttttta tatctgcaat
    63001 taattcgata aacagaccgt gacacatcac agcctgttta ttttctgtta tcagaacgtc
    63061 cagaccacac ccgcctgagt attccacggc gattctaccc ctgcgccatt gccatagctg
    63121 actgacaaat gaccgcttaa cgtcggggta aacgatgacc ttatcccagc ctgataaacg
    63181 ccactggttc cagccacatc attattaaat ttgccatcgt cattaacttt cacctggtta
    63241 gaatcggcgt attcctgacg cacggcggct ttcagccagg gttccagcgt cgtaccgttt
    63301 tgcaggtcca tgtgatagct taccgccgtt cccgcttcag cgcgtaatat ccgggtattt
    63361 cccacgtccg cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt ggtaaagccg
    63421 gtaaaggcca gatagggtct aacactccac aatccgtcaa cccaacggaa cccgctctca
    63481 acatgagcac ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc attactcatc
    63541 ttgccatgga tggtgttggc aaaacggtca actttcacca ccccatcaac ataggcaccg
    63601 ttctgatgct cccaaccggc ataagccccc agggtatagc tatcgatatt accttttccg
    63661 ccgcgatcaa aaccaatatc agaatgagag taaccaaaga acaagccgcg aattgtactg
    63721 ctttcttcac gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca
    63781 aaaccagctc ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga actccacatc
    63841 gccgtatcgt aactaacgcc ttttacgcta ccaagacgct cacgcacggt gtccagttct
    63901 gcatcaaata ccagcggttg tgctgccgcc atatttagca catcagtggt tgaaggggta
    63961 atttgcgcgc gattctctgc cagactccag ctatggttac cattatccag caaggtatat
    64021 tcatacgtac cgatatcaac aacgcctccg gcattgccca acgtaaatgc agcatcaccg
    64081 ccgcccgttg ttaccagtgt aaggctatct cctgctgccg ggctggcacc ggtgtccgtc
    64141 acgaatattt tgaaatcacc tgttgcctga ccggtgacgt tgagctgatc gctctgatga
    64201 ttagccatat cggtacgcat ataaaaattg ccgtttcccg acagggtatt ggtggtcaac
    64261 gtaatgaaat tttccgcact tgccgtcact gatgaacgag tcactggctc atccatcata
    64321 atcacatttg cgtcagacat cgcaaggttg gctaagttga ggagggtgtt ttggttcgtc
    64381 gtagtgatat cggcaatgat ggtccatacg ctattttcga gcgaaacgtc cgctttgctg
    64441 gtatccacgg cgccatgcgt tctaaaagaa gcgccctcag aaaccactac cttacccacc
    64501 aacattgcgg gtgcataatt tatttgcggc ttcatgacct caagaatgcc atcattccct
    64561 ctgactgaaa cgttaaccat tgtgccagcc cagacgttag cgcggccatt gttgataacc
    64621 atattttcag agatggcttt tgaggaatac gtgatacttc cattctgatc tgatcgaccg
    64681 agatcatata ccgcatttgc ctgcactctc gtcccagtat cctttcccag cgattgcata
    64741 gtggcatgct catcaaggat agtgtcaatg gcctgcgtgt cctccataac aataagcccg
    64801 gaaccatcat ccagttcata attttttgac acaccatctt ttatactaaa ttggccttta
    64861 ctgtttgtac cactcacttc cagcgcattc gttgagacaa ttaattttcc gcctgctttt
    64921 ttatcaacgc cagttgcagt cccgccatcc ataacctcca gtaagccgcc actatctaca
    64981 gtagtattat aagcgaagtc attttcttca actcgcaaac ttccgccgtt ttccaacagc
    65041 atattgtttg caattccatt cttgatatcg aactgaccga gacggtttgt tccgaatacc
    65101 tccatgaccc gcgtggttgc tttaatagca ccgcctgctt tctgatctac cgcaatcgct
    65161 aatcccccct ctttaacctc cagtaatcct cccttttcga tagcggcaag gacggtaatc
    65221 ccatcttttt caacgattaa gcgcccgcct ttaatcgtgg gatcattcgc cgcgccataa
    65281 acctgtagca caccattatt gagggtagtc tggtttgccg cgccacccat ttgaacaatt
    65341 tgataaccat catttaatac ggagtattct gcggtgccat tcatttcaat gtactgatac
    65401 ccgccgttaa tttcagtatt actacttata ccaaattctt taatatgctg ttctccgcca
    65461 ggattgattt tcgtattatt ggcggtccca taaaccagct ggatatcttt ttccaggaca
    65521 acaccatcaa ctgtctcacc atactcaact gttgatgcca atgctatggg tgaatataac
    65581 gctgtagcca ccagaacaga tattgcggaa cgacaaagag aaacagaacc agattgatgc
    65641 attgagcttt catcctatga aattaattgc tgttaaaagc attgggtaca gaaaataccc
    65701 atagctccat acccggaatc agtttttaaa aactgtttaa agaaatgcac aagtattgtg
    65761 attgattttt tagttgtttt tcttgatgag aagctgatgc aaaattccgt ctttataatg
    65821 aaaatgatgc caaagcgaac ggcaaggttg tagttttcac tacatgtcca tacataaaat
    65881 ggggtaacat tcacgcgcct ggtagcgtta ccaacgctac gctcaaacat aatgattcta
    65941 ataaaacctc aggagactac tatgcctgaa gcaacgcctt ttcaggtgat gattgtggat
    66001 gatcatccac ttatgcgacg cggtgttcgt cagttactgg agcttgatcc tggctttgaa
    66061 gtggtcgccg aagcgggcga cggcgcgagc gcgatcgatc tggcgaatag actggatatc
    66121 gacgtgatct tgctggatct gaatatgaaa ggtatgagtg gcctggatac cctcaatgcc
    66181 ttgcgcaggg atggcgttac cgcgcaaatt attatcttga ccgtatcaga tgcctccagc
    66241 gatgtctttg cactgataga cgcaggcgca gacggttatc tgttgaaaga cagcgacccg
    66301 gaagtgttgc tggaagcgat tcgtgccgga gcgaaaggca gcaaagtctt tagcgaacgc
    66361 gtcaatcagt acttacatga acgtgaaatg tttggcgcgg aagaagatcc cttcagcgtg
    66421 ctgacggagc gcgagctgga tgttctgcac gagctggcac aggggctgtc aaataaacag
    66481 attgcctcgg tgttgaatat ttcagagcag acagtaaaag tacatattcg caatctgctg
    66541 cgcaaactca atgtccgctc acgcgtggca gccaccattc tgttcctgca acaacgcggg
    66601 gcacaataaa aatagcccga tggcatcacc atcgggcctc tttttattta ctctcctgcg
    66661 gcgacaaatg ttgcatcgcc tgcgcgatac tacgttcaat caccgcacgg cgagtatcgt
    66721 tggcaggtaa gagtttcaac atcatctccc acgcggcaac ggcttcgcca aatcgctgct
    66781 gctcaaaggc attaaacgca tacatgctta acacacgaat attgctatgg tccgttctca
    66841 ccaactggcg cagcagttcg ccgccgaggc ggttgtcgtt gggatcagat gaacgtgtca
    66901 acgcttcagc ataacccagt gcggcatcgc tgtttttcgg atcgaggcga tacgcagtag
    66961 cgtatgcatc ggtggcgata ctggcgttac ccagcgccat gccaacgcga cccaacataa
    67021 tccaaccttc tatatctccc ggattttttt gcagttgagt acgcatcccc agcgcaaggc
    67081 gcgacatctc ttcttcgttg agcggatcgg ctttcggatc cagcgccctg tccagcaacg
    67141 ccggagcctg tgccgtggcc tgctgccaga ttttcacctg ctgataattg ccagtctggt
    67201 agtagctgac gccagccaca attaacgcca ccacaatacc cggcagataa acaacatatc
    67261 cggcacgctt accttccggc acgctttgtt caggaaacgc ttccagcacc acgcgtaccc
    67321 gacgccgcga gcgggcgtaa atgacccaac cgccaatgcc aatagccact actggcagca
    67381 cccacagcag cacggtcagc ggcgttaacg gcggatcgta agtgacgaag ttgccgtaac
    67441 gcgccaccat ataatcgaca atctctttct tacttttacc ttcctgcatc agttcataca
    67501 ctttctggcg caggtcggtg gcaatcatcg agttggaatc ggcaatgctg ttattctgac
    67561 atttcgggca gcgcagttct tcagtgagct gacggaactg ttgttcctgt gcttcatctt
    67621 taaactgcaa cacgtcgatg gtcgccagcg ctgagccgga gatcatcagc atcagcacgc
    67681 ccaataaaaa cctcattgtg cggcctcctt actgtatttc tcccacagcg gcttgatctc
    67741 ttcttcccag acgcgaggat tcagatcgcc cgcatggcga tagcgaatga tgccgttgcc
    67801 gtcaataagg aacgtttctg gcgcgccata gacaccgaga tccagcccta acatgccgtc
    67861 gccatcaaac aggcttagcg cgtaaggatt gcccagctct ttcagccagc tgattgcctt
    67921 ctggcgatcg tctttatagt tcatgccgac cacgcggatg ccctgcgcag aaagctgatt
    67981 cagatattga tgttccgcac ggcaggtcgg acaccaggtc gcccagacgt taagcagcac
    68041 tggtttgccc tgagtcagca catccgcctg ataaaactgc cccggattgt ccagtgattc
    68101 gaggcgaaac ttcggcacag gcttgccaat gagcgccgat tccagattgg tcggatcatc
    68161 cccttcggca ttacgcgcca actgccacag cagcgccgcg gcaatcgcca ggaagataat
    68221 caaaggaatt aacaatactt tgcgcttcat acagcctccg gcgcagtttt ctgcgggttc
    68281 acgcgcttac gatagcgag
//
LOCUS       DAZGBC010000008        65283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGBC010000008 DAZGBC010000000
VERSION     DAZGBC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.008"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..568)
                     /gene="folK"
                     /locus_tag="ACHK1J_000517"
     CDS             complement(89..568)
                     /gene="folK"
                     /locus_tag="ACHK1J_000517"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO7909855.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIESQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVPQPAIW"
     gene            complement(565..1962)
                     /gene="pcnB"
                     /locus_tag="ACHK1J_000518"
     CDS             complement(565..1962)
                     /gene="pcnB"
                     /locus_tag="ACHK1J_000518"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO7909856.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2022..2918)
                     /gene="gluQRS"
                     /locus_tag="ACHK1J_000519"
     CDS             complement(2022..2918)
                     /gene="gluQRS"
                     /locus_tag="ACHK1J_000519"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO7909857.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPGNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2985..3440)
                     /gene="dksA"
                     /locus_tag="ACHK1J_000520"
     CDS             complement(2985..3440)
                     /gene="dksA"
                     /locus_tag="ACHK1J_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO7909858.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3618..4322)
                     /gene="sfsA"
                     /locus_tag="ACHK1J_000521"
     CDS             complement(3618..4322)
                     /gene="sfsA"
                     /locus_tag="ACHK1J_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO7909859.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEDQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4337..4867)
                     /gene="thpR"
                     /locus_tag="ACHK1J_000522"
     CDS             complement(4337..4867)
                     /gene="thpR"
                     /locus_tag="ACHK1J_000522"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO7909860.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCIQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4941..7370
                     /gene="hrpB"
                     /locus_tag="ACHK1J_000523"
     CDS             4941..7370
                     /gene="hrpB"
                     /locus_tag="ACHK1J_000523"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO7909861.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRMEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGISLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVRRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7566..10100
                     /gene="mrcB"
                     /locus_tag="ACHK1J_000524"
     CDS             7566..10100
                     /gene="mrcB"
                     /locus_tag="ACHK1J_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO7909862.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10320..12509
                     /gene="fhuA"
                     /locus_tag="ACHK1J_000525"
     CDS             10320..12509
                     /gene="fhuA"
                     /locus_tag="ACHK1J_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO7909863.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTIEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12560..13357
                     /gene="fhuC"
                     /locus_tag="ACHK1J_000526"
     CDS             12560..13357
                     /gene="fhuC"
                     /locus_tag="ACHK1J_000526"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO7909864.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLEAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVESLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13357..14247
                     /gene="fhuD"
                     /locus_tag="ACHK1J_000527"
     CDS             13357..14247
                     /gene="fhuD"
                     /locus_tag="ACHK1J_000527"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO7909865.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14244..16226
                     /gene="fhuB"
                     /locus_tag="ACHK1J_000528"
     CDS             14244..16226
                     /gene="fhuB"
                     /locus_tag="ACHK1J_000528"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO7909866.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFLQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16261..17541)
                     /gene="hemL"
                     /locus_tag="ACHK1J_000529"
     CDS             complement(16261..17541)
                     /gene="hemL"
                     /locus_tag="ACHK1J_000529"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO7909867.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAGSVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17707..17754
                     /gene="yadX"
                     /locus_tag="ACHK1J_000530"
     CDS             17707..17754
                     /gene="yadX"
                     /locus_tag="ACHK1J_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO7909868.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17766..19187
                     /gene="clcA"
                     /locus_tag="ACHK1J_000531"
     CDS             17766..19187
                     /gene="clcA"
                     /locus_tag="ACHK1J_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO7909869.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19211..19276
                     /gene="yadW"
                     /locus_tag="ACHK1J_000532"
     CDS             19211..19276
                     /gene="yadW"
                     /locus_tag="ACHK1J_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO7909870.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19269..19613
                     /gene="erpA"
                     /locus_tag="ACHK1J_000533"
     CDS             19269..19613
                     /gene="erpA"
                     /locus_tag="ACHK1J_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO7909871.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19660..20283)
                     /gene="yadS"
                     /locus_tag="ACHK1J_000534"
     CDS             complement(19660..20283)
                     /gene="yadS"
                     /locus_tag="ACHK1J_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO7909872.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20321..21121)
                     /gene="btuF"
                     /locus_tag="ACHK1J_000535"
     CDS             complement(20321..21121)
                     /gene="btuF"
                     /locus_tag="ACHK1J_000535"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO7909873.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSSEQVLARSPQAIVITGGP
                     DQIPKIEQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21114..21812)
                     /gene="mtnN"
                     /locus_tag="ACHK1J_000536"
     CDS             complement(21114..21812)
                     /gene="mtnN"
                     /locus_tag="ACHK1J_000536"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO7909874.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21896..23413
                     /gene="dgt"
                     /locus_tag="ACHK1J_000537"
     CDS             21896..23413
                     /gene="dgt"
                     /locus_tag="ACHK1J_000537"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO7909875.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23543..24967
                     /gene="degP"
                     /locus_tag="ACHK1J_000538"
     CDS             23543..24967
                     /gene="degP"
                     /locus_tag="ACHK1J_000538"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO7909876.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25122..26279
                     /gene="cdaR"
                     /locus_tag="ACHK1J_000539"
     CDS             25122..26279
                     /gene="cdaR"
                     /locus_tag="ACHK1J_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO7909877.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26368..26754)
                     /gene="yaeH"
                     /locus_tag="ACHK1J_000540"
     CDS             complement(26368..26754)
                     /gene="yaeH"
                     /locus_tag="ACHK1J_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO7909878.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27068..27892)
                     /gene="dapD"
                     /locus_tag="ACHK1J_000541"
     CDS             complement(27068..27892)
                     /gene="dapD"
                     /locus_tag="ACHK1J_000541"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO7909879.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVYIGQSTKIYDRE
                     TGEVHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27923..30595)
                     /gene="glnD"
                     /locus_tag="ACHK1J_000542"
     CDS             complement(27923..30595)
                     /gene="glnD"
                     /locus_tag="ACHK1J_000542"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO7909880.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30657..31451)
                     /gene="map"
                     /locus_tag="ACHK1J_000543"
     CDS             complement(30657..31451)
                     /gene="map"
                     /locus_tag="ACHK1J_000543"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO7909881.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31819..32544
                     /gene="rpsB"
                     /locus_tag="ACHK1J_000544"
     CDS             31819..32544
                     /gene="rpsB"
                     /locus_tag="ACHK1J_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO7909882.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32679..33530
                     /gene="tsf"
                     /locus_tag="ACHK1J_000545"
     CDS             32679..33530
                     /gene="tsf"
                     /locus_tag="ACHK1J_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO7909883.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDSGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33677..34402
                     /gene="pyrH"
                     /locus_tag="ACHK1J_000546"
     CDS             33677..34402
                     /gene="pyrH"
                     /locus_tag="ACHK1J_000546"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO7909884.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34552..35109
                     /gene="frr"
                     /locus_tag="ACHK1J_000547"
     CDS             34552..35109
                     /gene="frr"
                     /locus_tag="ACHK1J_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO7909885.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35201..36397
                     /gene="ispC"
                     /locus_tag="ACHK1J_000548"
     CDS             35201..36397
                     /gene="ispC"
                     /locus_tag="ACHK1J_000548"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO7909886.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36586..37344
                     /gene="ispU"
                     /locus_tag="ACHK1J_000549"
                     /gene_synonym="uppS"
     CDS             36586..37344
                     /gene="ispU"
                     /locus_tag="ACHK1J_000549"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO7909887.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37357..38214
                     /gene="cdsA"
                     /locus_tag="ACHK1J_000550"
     CDS             37357..38214
                     /gene="cdsA"
                     /locus_tag="ACHK1J_000550"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7909888.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38226..39578
                     /gene="rseP"
                     /locus_tag="ACHK1J_000551"
     CDS             38226..39578
                     /gene="rseP"
                     /locus_tag="ACHK1J_000551"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO7909889.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39608..42040
                     /gene="bamA"
                     /locus_tag="ACHK1J_000552"
     CDS             39608..42040
                     /gene="bamA"
                     /locus_tag="ACHK1J_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO7909890.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVQVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42162..42647
                     /gene="skp"
                     /locus_tag="ACHK1J_000553"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42162..42647
                     /gene="skp"
                     /locus_tag="ACHK1J_000553"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO7909891.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42651..43676
                     /gene="lpxD"
                     /locus_tag="ACHK1J_000554"
     CDS             42651..43676
                     /gene="lpxD"
                     /locus_tag="ACHK1J_000554"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO7909892.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43781..44236
                     /gene="fabZ"
                     /locus_tag="ACHK1J_000555"
     CDS             43781..44236
                     /gene="fabZ"
                     /locus_tag="ACHK1J_000555"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO7909893.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44240..45028
                     /gene="lpxA"
                     /locus_tag="ACHK1J_000556"
     CDS             44240..45028
                     /gene="lpxA"
                     /locus_tag="ACHK1J_000556"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO7909894.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45028..46176
                     /gene="lpxB"
                     /locus_tag="ACHK1J_000557"
     CDS             45028..46176
                     /gene="lpxB"
                     /locus_tag="ACHK1J_000557"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO7909895.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTEYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46173..46769
                     /gene="rnhB"
                     /locus_tag="ACHK1J_000558"
     CDS             46173..46769
                     /gene="rnhB"
                     /locus_tag="ACHK1J_000558"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO7909896.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46806..50288
                     /gene="dnaE"
                     /locus_tag="ACHK1J_000559"
     CDS             46806..50288
                     /gene="dnaE"
                     /locus_tag="ACHK1J_000559"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO7909897.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50301..51260
                     /gene="accA"
                     /locus_tag="ACHK1J_000560"
     CDS             50301..51260
                     /gene="accA"
                     /locus_tag="ACHK1J_000560"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO7909898.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51359..53500
                     /gene="ldcC"
                     /locus_tag="ACHK1J_000561"
     CDS             51359..53500
                     /gene="ldcC"
                     /locus_tag="ACHK1J_000561"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO7909899.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53557..53946
                     /gene="yaeR"
                     /locus_tag="ACHK1J_000562"
     CDS             53557..53946
                     /gene="yaeR"
                     /locus_tag="ACHK1J_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7909900.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54011..55309
                     /gene="tilS"
                     /locus_tag="ACHK1J_000563"
     CDS             54011..55309
                     /gene="tilS"
                     /locus_tag="ACHK1J_000563"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO7909901.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(55358..55618)
                     /gene="rof"
                     /locus_tag="ACHK1J_000564"
     CDS             complement(55358..55618)
                     /gene="rof"
                     /locus_tag="ACHK1J_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO7909902.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55605..55805)
                     /gene="yaeP"
                     /locus_tag="ACHK1J_000565"
     CDS             complement(55605..55805)
                     /gene="yaeP"
                     /locus_tag="ACHK1J_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO7909903.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55971..56516
                     /gene="yaeQ"
                     /locus_tag="ACHK1J_000566"
     CDS             55971..56516
                     /gene="yaeQ"
                     /locus_tag="ACHK1J_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO7909904.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56513..56935
                     /gene="arfB"
                     /locus_tag="ACHK1J_000567"
     CDS             56513..56935
                     /gene="arfB"
                     /locus_tag="ACHK1J_000567"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO7909905.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56949..57659
                     /gene="nlpE"
                     /locus_tag="ACHK1J_000568"
                     /gene_synonym="cutF"
     CDS             56949..57659
                     /gene="nlpE"
                     /locus_tag="ACHK1J_000568"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO7909906.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQVAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57859..58683)
                     /gene="yaeF"
                     /locus_tag="ACHK1J_000569"
     CDS             complement(57859..58683)
                     /gene="yaeF"
                     /locus_tag="ACHK1J_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO7909907.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQAIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQICSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58736..60454)
                     /gene="proS"
                     /locus_tag="ACHK1J_000570"
     CDS             complement(58736..60454)
                     /gene="proS"
                     /locus_tag="ACHK1J_000570"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO7909908.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60565..61272)
                     /gene="tsaA"
                     /locus_tag="ACHK1J_000571"
     CDS             complement(60565..61272)
                     /gene="tsaA"
                     /locus_tag="ACHK1J_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO7909909.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MTVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61269..61673)
                     /gene="rcsF"
                     /locus_tag="ACHK1J_000572"
     CDS             complement(61269..61673)
                     /gene="rcsF"
                     /locus_tag="ACHK1J_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO7909910.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61791..62606)
                     /gene="metQ"
                     /locus_tag="ACHK1J_000573"
     CDS             complement(61791..62606)
                     /gene="metQ"
                     /locus_tag="ACHK1J_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO7909911.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62646..63299)
                     /gene="metI"
                     /locus_tag="ACHK1J_000574"
     CDS             complement(62646..63299)
                     /gene="metI"
                     /locus_tag="ACHK1J_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO7909912.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63292..64323)
                     /gene="metN"
                     /locus_tag="ACHK1J_000575"
     CDS             complement(63292..64323)
                     /gene="metN"
                     /locus_tag="ACHK1J_000575"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO7909913.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64511..65086
                     /gene="gmhB"
                     /locus_tag="ACHK1J_000576"
     CDS             64511..65086
                     /gene="gmhB"
                     /locus_tag="ACHK1J_000576"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO7909914.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFINGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15453 a        16175 c        17963 g        15692 t
ORIGIN      
        1 gtaagataag aaaataattt ttcttggtgg atgaaatagc cgtatcattt tgtattcctc
       61 tcgtcaagaa aataaaataa tacagctatt accagatggc aggttgtggt actgctagtg
      121 aagtgagtat ttcacgtaat gtttccccat caggaaacac caactccggc gcgatttcaa
      181 acagcggcca cagcataaat ccacgatttt tcatatcgta gtgcggaacg gtcaggcgtt
      241 cggtatttat tacttcatta ccaaacagca tgatgtcgag atccagcgtg cgcggtcccc
      301 agcgttcagc tttacggacg cgaccttgct gcgattcaat acgctgtgtg tgattgagta
      361 gctcttcagg tgcaagagag gtttccagcg ctacggctgc atttaagtaa tcgggttgat
      421 cttgcggccc cagcggtgga gtgcggtaaa acgaagaaac ggcaagaatg tggctttcag
      481 ggatatcgcc taatgctttc agggcagcat tgacctgctc cagcggagag gccagattgc
      541 tgcctatggc aatatacgcc actgtcatgc ggtaccctca cgacgtggtg cgcgtttgcg
      601 tggacgacga gtacgacgac gcggtgacgg ttcttcatcc agctcgttga gcatcccttt
      661 ttggtctggt ggcgcggaaa cctggaactc accccaccat ttcaccagac gctgcagttc
      721 agcgttacgt tcaacttcag ctcgcaaggc caacaggtca taagccgcac ggaacttagg
      781 atgctccagc agtttccatg cacgtttacc ctgacgtcgg gacatacgca actgcaactg
      841 ccagatatcg cgggttaacg tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc
      901 gtccagcacg tcgttcatcg ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg
      961 ggcgatcttc tgtgccgtct ccagcagtgg gtaccagaac atggcggcaa acaaaaacgc
     1021 cgggttaacg cgcatatcgt tatgaatacg cgtatcggtg ttcttcagta cctgctcaat
     1081 gatccgctcc attgggctgt cgccattttc cgtgaagtag cgggtaatgg tcgggaacag
     1141 cggctggaac agatgatatt cacacagcag cttataggtt tcgtaaccgt agcccgcttg
     1201 tagcagttta agcgattctt caaacaggcg tgccggtggg atatcgttca gcagggtagc
     1261 gaggcgaggg atcggttctg cggtttccgg gctgatgcgc atacccaatt tggcggcaaa
     1321 acgtactgcg cgcagcatac gtaccggatc ttcacggtag cgcgtttccg ggttaccaat
     1381 cagacggata acaccgtcct tcagatcctt cataccgcca acgtaatcac ggacggtaaa
     1441 atccgctacg ctgtaataca ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc
     1501 gatggagccg aaaatgttgt cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt
     1561 gcggtcgctg acgttacctt cgtggtgtcc acggaaggtc gcaacttcga taatctccgg
     1621 gccaaacatc acatgagcca gacggaaacg gcgacccacc aggcggcagt tacggaacag
     1681 tttgcgcacc tgctcaggcg tggcgttagt ggttacgtca aagtctttcg gctttttgcc
     1741 aagtaacagg tcgcgcacgc cgccgccaac cagccaggct tcgtatcccg ctttattgag
     1801 cctgtacatt accttcaggg cattttcact gatatctttg cgggaaatag catgctgctc
     1861 acgcgggatc accgtcacct gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg
     1921 gcttagcacc ttgcggcaaa aattagcgac tcgggtaaaa atagtacacc tcggtagtgt
     1981 caaacatcat tcaggacaaa aaaatagcgg ctaatcatag ctcagcatga cgcatttgag
     2041 aatgttgaat ttacaattgc cgactcaggc actgcggtaa gccgccagtt tttgacggct
     2101 gactgaagga tttgttcgac gctgaaatcc tgccagtgtg cttctacctg ctgacccaga
     2161 aattgaagtg ccgcgattag taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga
     2221 ttctgcttgg aaagtttagc gccttgtgga ttaagcgcca gcggcagatg aatgtaatct
     2281 ggcactttcc agccaaaaag ctggtacagc gagatttgcc gcactgtcgg ttcgatcaaa
     2341 tccgccccgc ggacaatttc gctcacgccc tggaagtgat catccaccac caccgccagg
     2401 ttataggcaa acaaaccatc acggcgatga ataataaaat cttcccgtgc cagtttttcg
     2461 tcggcgtgaa taatgcctcg cagcaggtca gtaaactggg tgaccggatg ctgctggcgg
     2521 atacgcactg cggcgttgcc tggtccatga tgcaattccc ggcaatgacc gtcgtaaata
     2581 ccgccaatgc tttgaatacg tgcacgcgtg caggtgcagt aataacttag tccttgctca
     2641 tgtaaccagg cgagtgcttc gcgataggcg tggtgacgtt gcgattgcca gagaacatcg
     2701 ccgtcccagt gcaggccgta atgttccagc tggcgcagga tagtttctgc ggcaccggga
     2761 acttcacgag gcgggtcgat atcttctatg cgtaccagcc agcgaccttg ccgggcgcga
     2821 gcctgcaaat agctgccaag cgcagcaatc agagaaccaa aatgaagctc gccggaagga
     2881 gagggggcga agcggccaat atagtgtgtg tctgtcatct ctttgaacaa aaaataaggc
     2941 gggagcattt cccgcctgtg gtaaacgtga tggaacggct gtaattagcc agccatctgt
     3001 ttttcgcgaa tttcagccag cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct
     3061 tccagacggc gaataccaat ttcaacaccg caggattcac agtagccgaa atcttcgtct
     3121 tccacttttt tcagcgtctt ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta
     3181 cgcagttcga ggctgaactc ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg
     3241 gctgcttcat cctgcatatg tgtaacggtg cgatcgactt catccctgag ttgattacgc
     3301 catgcttcca gaatacgacg gaagtgcgcc agctgggctt cattcatata ctcttcgccc
     3361 ggcttctcct gatatggttc caccccagcg atggcgagaa tactcaggga cgatgtttta
     3421 cggttttgcc cttcttgcat gttgcttctc cttaacacgc actatcgatc cccatgttcg
     3481 ggggaaaaat gaggccgcta taaatagcag atgcttttcc ggatagcaat tatctaaacg
     3541 taacacttga caactgtgtg aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta
     3601 accagttact tactttacta caatgtaacc ggcagtgatt ttttaagagc catgccttca
     3661 gcagaaattt ccgctttgta agccagaatt tctacccccc tctgttgagc ttctgacaat
     3721 agttgcgcgt atttctcatc gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc
     3781 agcacggcga aaaagataac cgcacgctgg tcttcagccg ctacgctcat caactcccga
     3841 aggtgtttct gacctcgttc agtgaccgca tcgggaaaat atccctgttc gttctccgct
     3901 aacgtaaccg atttcacttc aatatagcag tctggacgcg aatccgcctg caacataaag
     3961 tcaatacggc tgcgttctgc gccgtatttt acttcgcttt tcagcgagct atagcctgac
     4021 agttctgaaa ttgattcatt aaggatagcc tctttcgtca acctgttagc ccaaagcgtg
     4081 ttgacgcaaa taaatgcgcc gctctggctt tgagttaatt cccaggtgtg tgggtatttc
     4141 cgtttagtgt tgtctgaagt cgaataccag acggtatcgc caggcgttgc acaaccggtc
     4201 atcgcacccg tattcgggca gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg
     4261 gctaaaaaac gtttgtaacg ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc
     4321 attgcgacaa tccttgttat tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc
     4381 gtccacgggc aaacgaggag gcgtaaaggg tgaactccgt caccgcatac gaccagttaa
     4441 aacctggcgg cgggattgtc accgcctcgc tggcgtcgcg caataaggta atatgtggat
     4501 gaaacggacg attgctttga atacaaccgc tgcgggcagc ctgtgaacgg agcatattcg
     4561 ccagctggat taagccgcgt ggaggctgac gcatccctaa ccacaccaca cgcgaacgca
     4621 gccattgtcc ggcgtcatca agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta
     4681 aaagagaaag cgccttctct ttctcggcgc tcacttcgcc taaaaatgcc agagtcagat
     4741 gcaaattatc ggcggcgacc ggacgtcccg cctcaagtgg gaagtgtgtg gcgcgccagt
     4801 ggataatctg ttcgcggatt tctgcaggta agtcgatagc aaagaacaga cgttgcgggt
     4861 cagacatgtg aggcactcgg ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg
     4921 ttaaccctct ggagcgtttt gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac
     4981 ttaccgctct cgattgtgcg ccgcaggtat tattaagtgc gccgaccggg gccgggaaat
     5041 caacctggct gccgctgcaa ctgctggcgc atcccggcat taacgggaaa attatcctgc
     5101 tggagccgcg tcgtctggcg gcgcgtaacg tcgcgcaacg gctggcggaa ctgcttaacg
     5161 aaaagccagg cgataccgtt ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata
     5221 cccgcatgga agtagttacc gaaggcgtgc tgacgcggat gatccagcgt gacccggaac
     5281 tgagcggtgt tggactggtg atccttgatg aatttcatga gcgcagcttg caggcggatt
     5341 tggcgttggc gctgttactc gatgtgcaac aaggtctgcg tgatgacctt aaactgctga
     5401 ttatgtcggc tacgctggat aacgaccgct tgcagcaaat gctgccagaa gcgcccgtcg
     5461 tcatctcaga agggcgctcg tttcctgttg aacgccgtta tttaccgctg cccacgcatc
     5521 agcgttttga cgaagccgtc gcggttgcca ccgccgaaat gctgcgtcag gaaagtggat
     5581 cattactgtt atttttacct ggcgtcggag aaattcagcg tgtgcaggaa caactggctt
     5641 cgcgcatcgg cagtgatgtg ttgctctgcc cgctgtatgg cgcgttgtcg ctgaacgatc
     5701 agcgaaaagc gatcctcccg gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata
     5761 ttgctgaaac cagtttaacc attgaaggta ttagtctggt ggtggattgt gcccaggagc
     5821 gtgtggcgcg ttttgatccg cgcacggggc ttacgcgact gattactcaa cgcgttagcc
     5881 aggcatccat gacgcagcgt gccgggcgcg ccgggcgtct ggagccgggt atctgcctgc
     5941 atttaatcgc caaggaacaa gcagaacgcg ccgcggcgca aagtgaaccg gagatcttac
     6001 aaagcgatct ttccggtttg ctgatggaat tactgcaatg gggatgcagc gatccggcgc
     6061 agatgagctg gctggatcaa ccgccaacgg tgaatctact ggccgcgaaa cgtctgttac
     6121 aaatgctggg ggcgctggac ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc
     6181 tgggtaacga tccgcgttta gcggcaatgc tggtgagcgc gaagagcgac gacgaagctg
     6241 ctaccgcggc aaaaattgcc gccattctcg aagagccgcc acggatgggc aatagtgacc
     6301 tgggcgtggc gttttcgcgc aatcaaccag cctggcagca acgtagtcag caactgttaa
     6361 aacgcttaaa cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg ctacttgccg
     6421 gggcgtttgc cgatcgcatt gctcgccgcc gtgggcaaga tggacgctat caactggcaa
     6481 acggcatggg agcgatgctc gatgccgacg acgcgctaag ccgccacgaa tggttgatcg
     6541 caccgttatt attgcagggc agcgcctcgc cggatgcgcg gattttactg gcgctaccgg
     6601 tcgatattga tgaattagtg cgacgctgtc cgcagctggt gcagcaatct gacaccgtgg
     6661 aatgggatga cgcgcaaggt acgctgaaag cctggcgtcg gctgcaaatc ggtcagttga
     6721 cggtgaaagt gcagccgttg gcgaaaccgt cagaagacga gttgcatcag gcgatgctta
     6781 acggcatccg tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg gaacagctac
     6841 gcttgcgttt gttatgcgcc ggaaagtggt tgccagaata tgactggcca gcggttgatg
     6901 atgaaagttt gttggcgacg ctggaaacgt ggctgctgcc acatatggcc ggtgtacatt
     6961 cactacgcgg cctgaaatca ctcgacattt atcaggcact acgcggatta cttgattggg
     7021 gaatgcagca acgtctggat agtgaattgc ctgcgcatta cactgtgccg acgggaagcc
     7081 ggatcgccat tcgttatcat gaagataacc cgcccgcgct ggcggtgaga atgcaggaga
     7141 tgtttggtga ggccaccaat ccgacgatcg cccaggggcg cgtgccgctg gtgctggagt
     7201 tgctttcccc tgcccaaagg ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag
     7261 gggcgtatcg tgaggtgcaa aaggagatga aagggcgtta tcccaaacac gtctggccgg
     7321 acgacccggc aaacacggca ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt
     7381 ctccctctcc ctgtgggaga gggccggggt gagggcatca gcgcgcacgt tcaccctcac
     7441 cctaaccctc tcccttaagg gagaggggac cgatcgagca caaattttga gagatatctt
     7501 cttctgtctt gtaacagaag aacagaaaat cgggcttttg cgcctgaata ttgcggagaa
     7561 aaagcatggc cgggaatgac cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg
     7621 tcaaacaaaa ggtaagccgt cgtcgttacg aagatgacga tgattacgac gattatgatg
     7681 actatgagga tgaagaaccg atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc
     7741 ctcgtggcaa acgcggctgg ctatggctgc tgttaaaact ggctatcgtt tttgccgtgc
     7801 tgatcgccat ttacggcgtt tatctcgatc aaaaaattcg tagccgtatt gatggcaagg
     7861 tctggcaact gcctgcggca gtttatggtc gaatggtcaa ccttgagcca gacatgacca
     7921 tcagcaagaa cgaaatggtg aagctgctgg aggcaaccca gtatcgtcag gtgtcaaaaa
     7981 tgacccgtcc tggcgaattt accgtgcagg ccaacagcat tgagatgatt cgccgtccgt
     8041 ttgatttccc ggacagtaaa gaaggacagg tgcgcgcgcg tctgaccttt gatggcgatc
     8101 atttggcgac gatcgtcaat atggagaaca accgtcagtt cggtttcttc cgtcttgatc
     8161 cgcgtctgat caccatgatc tcctcaccaa acggtgagca gcgtttgttt gtgccgcgca
     8221 gtggtttccc ggatttgctg gtggatactt tgctggcgac agaagaccgc catttctacg
     8281 agcatgatgg aatcagtctc tactccatcg gacgtgcggt gctggcaaac ctgacagccg
     8341 gacgcacggt gcagggggcg agtacgctga cgcaacagct ggtgaaaaac ctgttcctct
     8401 ccagcgagcg ctcttactgg cgtaaagcga acgaagctta catggcgctg atcatggacg
     8461 cgcgttacag caaagaccgt attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga
     8521 gtggcgacaa cgaaatccgc ggcttcccgc tggcgagctt gtattacttt ggtcgcccgg
     8581 tagaagaatt gagcctcgat caacaggcgc tgttagtcgg tatggtgaaa ggagcgtcca
     8641 tctacaaccc gtggcgtaac ccgaaactgg cgctggagcg acgtaatctg gtactgcgtc
     8701 tgctgcaaca gcaacagatt attgatcaag aactctatga catgttgagt gcgcgtccgc
     8761 tgggtgttca gccgcgcggt ggggtgatct ctcctcagcc agcctttatg caactggtgc
     8821 gtcaggagct tcaggctaaa ttaggcgata aggtaaaaga tctctccggc gtgaagatct
     8881 tcactacctt tgactcggtg gcccaggacg cggcagaaaa agccgccgtg gaaggcattc
     8941 cggcacttaa gaaacagcgt aagctgagcg atctggaaac cgcgattgtg gtcgtcgacc
     9001 gctttagtgg tgaagttcgt gcgatggtcg gaggttctga gccgcagttt gcgggctaca
     9061 accgtgcgat gcaggcgcgg cgttcgattg gttcccttgc aaaaccagcg acttatctga
     9121 cggctttaag ccagccgaaa atctatcgtc tgaatacgtg gattgcggat gcgccaatcg
     9181 cgctacgtca gccgaatggc caggtctggt caccgcagaa cgatgaccgt cgttatagcg
     9241 aaagcggcag agtgatgctg gtggatgcgt tgacccgttc gatgaacgtg ccgacggtaa
     9301 atctggggat ggcgctgggg ctgcctgcgg ttacggatac ctggattaaa ctgggcgtgc
     9361 cgaaagatca gctgcatccg gttccggcga tgctgctggg ggcgttgaac ttaacgccaa
     9421 tcgaagtggc gcaggcattc cagaccatcg ccagcggcgg taaccgtgca ccgctttctg
     9481 cgctgcgttc ggtaattgcg gaagatggca aagtgctgta tcagagcttc ccgcaggcgg
     9541 aacgcgctgt tccggcgcag gcggcgtatc tgacactatg gaccatgcag caggtggtac
     9601 aacgcggtac gggtcgtcag cttggggcga aatacccgaa cctgcatctg gcagggaaaa
     9661 cagggactac caacaataac gtagatacat ggtttgcggg cattgacggc agcacggtga
     9721 ccatcacctg ggtcggccgt gataacaacc agccgaccaa actgtatggt gccagcgggg
     9781 caatgtcgat ttatcagcgt tatctggcta accagacgcc aacgccgctg aatcttgttc
     9841 cgccagaaga tattgcagat atgggcgtgg actacgacgg caactttgtt tgcagcggtg
     9901 gcatgcgtgt cttgccggtc tggaccagcg atccgcaatc gctgtgccag cagagtgaga
     9961 tgcagcagca gccgtcaggc aatccgtttg atcagtcttc tcagccgcag caacagccgc
    10021 aacagcaacc tgctcagcaa gagcagaaag acagcgacgg tgtagccggt tggattaaag
    10081 atatgtttgg tagtaattaa cgtctaaacg tgacataccg gatggcgagt tgccatccgg
    10141 taaaataaca tcccacctaa agtattaacc ctttcttttc atctggttgt ttattaaccc
    10201 ttcaggaacg ctcagattgc gtaccgcttg cgaacccgcc agcgtttcga atattatctt
    10261 atctttataa taatcattct cgtttacgtt atcattcact ttacatcaga gatataccaa
    10321 tggcgcgttc caaaactgct cagccaaaac actcactgcg taaaatcgca gttgtagtag
    10381 ccacagcggt tagcggcatg tctgtttatg cacaggcagc ggttgaaccg aaagaagaca
    10441 ctatcaccgt tactgctgca cctgcgccgc aagaaagcgc atgggggcct gctgcaacta
    10501 ttgcggcgaa gcactctgct actgcgacta aaacggatac accaattgaa aaaacgccgc
    10561 agtctatttc ggttgtgaca aatgaagaga tgcagatgca tcaatttcag tctgtaaaag
    10621 aagcattagg ttatacacct ggtgttacgg ttagcagtcg tggtgcttct aatacatatg
    10681 attttgttat cattcgtggt ttctcatctg ttggtctgaa tcaaaataac taccttgatg
    10741 gactaaaact tcagggcaac ttttataacg atgctgtgat tgatccttac atgctcgaga
    10801 gggttgaact gatgcgtggg ccgacgtctg ttctctatgg aaaaagtaat cctggtggga
    10861 ttatttctat ggtgagtaag cgtccgacca ttgaacccct gaaagaaatt cagtttaaaa
    10921 tggggacgga taatctgttt cagaccggat tcgatttcag tgatgcgctg gatgataacg
    10981 gtgagttctc ctaccgtttg acggggctcg ctcgttcaac aaacgaacag cagaaaaatt
    11041 ctgaatctca gcgttatacc attgctccat cattctcatg gcgtccggac gacaaaacca
    11101 attttacttt cctgtcctat ttccagaatg aaccagaaac gggttattac ggttggttgc
    11161 cgaaagaggg gaccgttgag ccattgccta atggtaaacg tctaccgact gacttcaatg
    11221 aaggtgcatc gaataataca tactcccgta accagaaaat ggtgggatat agttttgaac
    11281 atggttttaa tgacaccttc accgtgcgtc agaatctgcg tttcagtgaa atgaaaacct
    11341 cacagaaaag tgtttatggc acagggattg ccaacgatgg tcatactcta aaccgcggga
    11401 cagtggtgga taatgagcgt ctgcaaaact ttagcgttga tacccaactt gaaagtaaat
    11461 ttgctacagg tgaagtcgag catactttgc tgacaggggt agacttcatg cgtatgcgca
    11521 atgatatcaa tgccagcttt ggatctgcac catccatcga tctttataac aaatatcatc
    11581 ctgaatactt tgcatttggt aacgcagagc cataccaaat gaatgaaagc aaacaaacag
    11641 gtatttatgt tcaggatcag gcggaatgga ataaatgggt attcactctg gggggacgct
    11701 acgattggtc taagcaagcg accactgttc gtgaaaactc ttatacgccg actgaaggtt
    11761 atattgagcg taatgatcat cagttcacct ggcgcggtgg tgtaaactac ttattcgata
    11821 atggtatttc accttacttt agctatagcc agtcctttga accgagtgct ttcgatctgt
    11881 ggagcaaccc gcgcgtttcc tataagccat cgaaaggtga acagtatgaa gctggcgtaa
    11941 aatatgttcc gaatgatatg ccggtcgttg ttacgggcgc agtctatcaa ttgacgaaaa
    12001 caaataacct gacagcagac ccaacaaacc cgttagcgca agtcccagca ggtgagattc
    12061 gcgctcgtgg tgtggaactt gaagcaaaag cggcgttaaa tgccaatatt aacttgacgg
    12121 cttcttatac ctacacggat gcggaataca ccaaagacac caatctcaaa ggtaaaactc
    12181 ctgaacaagt accggagcat atggcatctc tctgggggga ttataccttc aatgaagggc
    12241 cgctttctgg tttaacattg ggaacaggtg gtcgttttat tggttccagc tatggtgatc
    12301 cggcaaacac ttttaaagtg ggtagcgcag ctgtaatgga tgctgttgta aaatatgatc
    12361 tggcacgctt tggtatggcg ggatccagcc ttgctgtgaa cgtcaacaat ttgctcgatc
    12421 gtgagtatgt tgccagttgc ttccagacct atggctgctt ctggggcgca gaacgtcagg
    12481 tcgttgcaac cgcaaccttc cgtttctaat tttctctttg gggcacgggt ttccgtgccc
    12541 gcttcacaag ttggctgtta tgcaggaata cacgaatcat tccgatacca cttttgcact
    12601 gcgtaatatc tcctttcgtg tgccagggcg cacgcttttg catccgctgt cgttaacctt
    12661 tcctgccggg aaagtgaccg gtttgattgg tcacaacggt tctggtaaat ccacgctgtt
    12721 aaaaatgttg ggccgtcatc agccgccgtc agaaggtgaa attcttcttg aagcccagcc
    12781 gctggaaagc tggagtagca aagcgtttgc ccgcaaagtg gcttatttgc cgcagcaact
    12841 tcctccggca gaagggatga ctgtgcgtga actggtggcg attgggcgtt acccgtggca
    12901 tggcgcgctg gggcgctttg gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct
    12961 ggttggctta aaaccgctgg cgcatcggct ggtagaaagc ctctctggcg gcgaacgtca
    13021 gcgggcgtgg atcgccatgc tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc
    13081 gacttcggcg ttggatatcg cccaccaggt tgatgtgctg gcgctggtgc accgtttaag
    13141 tcaggagcgt ggcctgacgg tcattgccgt gctgcacgat atcaatatgg ctgcgcgtta
    13201 ctgtgattat ctggtcgccc tgcgcggcgg tgaaatgatt gctcagggaa cgcctgcgga
    13261 aattatgcgc ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta ttctgccgca
    13321 tccggcgggt gctgcacctg tgagttttgt ttattgatga gcggcttacc tcttatttcg
    13381 cgccgtcgac tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc
    13441 cacgcggcgg ctattgaccc aaatcgtatt gtggcgctgg agtggctgcc ggtggaatta
    13501 ctactggcgc tcggcatcgt gccttacggc gtggcggaca ccatcaacta tcgcctgtgg
    13561 gtcagcgaac caccactacc tgaatcggtg attgacgtcg gtttgcgtac agaacctaac
    13621 ctcgaactgc tgaccgaaat gaaaccgtcg tttatggtct ggtcggcggg atatggtcct
    13681 tcgccagaaa tgctggctcg tattgcgccg gggcgtggat ttaacttcag cgacggcaaa
    13741 cagccgctgg cgatggcgcg taaatcgctg acggaaatgg cggatttact taacctgcaa
    13801 agcgcagcag aaacgcattt agcgcaatat gaagacttta tccgcagcat gaaaccccgc
    13861 tttgtgaagc gtggtgcgcg tccgttattg ctgacgacgc ttatcgatcc gcgccatatg
    13921 ctggtcttcg gtccaaacag cttgttccag gaaattcttg atgagtacgg catcccaaat
    13981 gcctggcaag gggaaaccaa cttctggggc agtaccgccg tcagcatcga tcgtctggca
    14041 gcgtataaag acgttgatgt gctctgtttt gatcacgaca acagcaaaga catggatgcg
    14101 ctaatggcaa cgccgctgtg gcaggccatg ccgtttgtcc gcgccggacg ctttcagcgc
    14161 gtacccgcag tctggtttta tggtgcaacg ctctcggcaa tgcactttgt gcgcgttctg
    14221 gataacgcca ttggaggtaa agcgtgagta aacgtattgc gcttttcccg gcgttattgc
    14281 tggcgctgct ggtgattgtc gctacggcgc tgacctggat gaactttttg caggcgctgc
    14341 cgcgtagcca gtgggcgcag gccgcctggt cgccggatat taacgtcatc gagcagatga
    14401 tttttcacta cagcttgtta ccgcgtctgg cgatttctct actggtgggc gcgggcctgg
    14461 ggctggtggg cgtgctgttt cagcaagtgc tgcgtaaccc gctggcggaa ccgacgacgt
    14521 tgggcgttgc tacaggcgcg caactgggga ttaccgtcac gacgctctgg gcgatccctg
    14581 gtgcgatggc gagccagttt gctgcgctgg caggggcttg tgttgttggc ttaattgtct
    14641 ttggcgtcgc gtgggggaaa cggctgtcgc cggtaacgct gattctcgcg gggttggtag
    14701 tgagccttta ttgcggcgca atcaatcagt tactggttat cttccatcat gaccaactgc
    14761 aaagcatgtt tctgtggagc actggaacgc tgacgcaaac cgactggggc ggtgttgagc
    14821 gtttatggcc gcagttgctg ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa
    14881 cactgatggg gcttgatgat ggcgtggcgc gcaatctcgg gctagccttg tcgctggcgc
    14941 gtctggcggc gctgtcgctg gctattgtca tcagtgcgct gctggtgaac gcggtgggga
    15001 ttatcggctt tatcgggttg ttcgcgccgt tgctggcaaa aatgctgggg gcgcggcgtc
    15061 tgctgccgcg actgatgctg gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc
    15121 aaatcatcct ctggctgact cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt
    15181 tgatcggtgc gccgctgcta ctgtggttgc tgccgcgttt acgcagcatt agcgcgccgg
    15241 atatgaaggt caacgatcgt gtcgcggctg aacgccaaca tgtgctggcg tttgccctcg
    15301 cgggcggcgt gctgctgttg atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc
    15361 acggctggac gtgggcgagc ggggcgttgc tcgatgattt aatgccctgg cgctggccgc
    15421 gaattatggc ggcgctgttt gcgggcgtca tgctggcggt ggcgggctgt attattcagc
    15481 ggctgaccgg caacccgatg gcaagcccgg aagtgctggg gattagctct ggcgcggcgt
    15541 ttggcgtggt gttaatgctg tttctggtgc cgggtaatgc ctttggctgg ctgttacccg
    15601 cgggcagtat cggtgccgcg gtgacgctgt tgattattat gatcgccgcc gggcgcggtg
    15661 gattttcccc acaccgcatg ttactggcgg ggatggcgtt aagtaccgca ttcaccatgc
    15721 ttttaatgat gttgcaggcg agtggtgacc cgcgaatggc gcaagtgctg acctggatct
    15781 ctggttcgac ctacaacgcc accgatgcgc aggtctggcg caccggaatt gtgatggtga
    15841 ttttgctggc gattaccccg ctgtgccgcc gctggctgac cattttaccg ctgggtggtg
    15901 ataccgcgcg ggccgtgggg gtggcgttaa cgccgacgcg aattgcgctg ctgctgttag
    15961 cggcttgcct gacggcgacc gcgacgatga ccattggacc gttgagcttt gttggtttaa
    16021 tggcaccgca tatcgcgcgg atgatgggct ttcgacggac gatgccgcac atcgtgattt
    16081 cggcgctggt gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt
    16141 ttccgttcca gatcccggcg gggctgctgt cgacctttat cggcgcgcca tattttatct
    16201 atttgttgag aaagcagagc cgttaataaa atgcctcgcc cggtgggcga ggcaaaatca
    16261 tcacaacttc gcaaacaccc gacgtgcagc atcgatggtg ttattgatat cttccatgct
    16321 gtgcgccaca gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc
    16381 cagcatcata tggaagaaac gcttaaaacg ctcgacgtca caggccatca catcctgata
    16441 gcacgtcacg gacccggcgt cggtaaagaa aataccgaac atgccgccaa cgtggtttac
    16501 caccagcgga attccggctt cttctgccgc ttccagcaga ccttctgcca gacgtgttgt
    16561 cagctcatcc agcgtttcgt gaacgcccgg ctgcgcgact tcattcagac aggcgaaacc
    16621 ggctgccatc gcaatcgggt taccggaaag cgtacccgcc tgatagaccg gacccgtcgg
    16681 ggccagcgca tccattacat cacgacgacc accgaatgcg cctaccggca ttccaccgcc
    16741 gatgattttg ccgaggcagg ttaaatctgg cactacgccg taataatcct gtgcgccagc
    16801 tagcgctacg cggaaaccgg tcatcacttc atcgatgatc agcaacgcgc caaattcgtc
    16861 acacagcgca cgcagacctg gcaggaactc tggcagcggc ggaacacagt tcatattgcc
    16921 tgccaccggc tcgacgataa tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg
    16981 tacagaagcc agatcgttat aagtacaggt taaggtatgt ttggcgaaat ctgccggaac
    17041 gcccggcgag tttggctggc ctaacgtgag tgcgccagaa ccggctttta ccagcaggca
    17101 gtcagcgtga ccgtggtaac aaccttcaaa tttaataatt ttgtcgcgac cggtaaaacc
    17161 acgggccagg cggatggcgc tcatggtcgc ctcggtgccg gagttcacca tgcgcaccat
    17221 atccatggtc gggaccagtt cggtcaccag ttgcgccatt ttcacttcca tttcggtcgg
    17281 tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg gcattgcgga tcgccggatg
    17341 gttatggccc agcaccatcg gcccccagga accgacataa tcgatatagg ctttgccatc
    17401 aacatcgtac agataagcgc cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc
    17461 agtaaaggcg cgaacagggg agttcacacc gccagggatc agctcgcgcg ctgcgctgta
    17521 aagattttca gacttactca tggagggttc ctgattcgta gaaaaagtga atggctgcta
    17581 ttctatgtta ttcataacaa gttaaatact cgtcaaacat caggctgctt gtactggtgc
    17641 aatcctgatt tcttagagta taaaagtttt gtgcatttga aacattacgc tttgcaaagg
    17701 attttcatgg aacgtgcgag taaaatgccg tcatcttatt tgtatgacca ataagtgatc
    17761 attggatgaa aactgatact ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca
    17821 gacaactgat tcgccaactt cttgagcgcg ataaaacccc gttagccatt ttgtttatgg
    17881 cggcagtcgt cggcacgctt gtcgggctgg ctgcggttgc ttttgacaaa ggtgtcgcct
    17941 ggttgcagaa ccaacgtatg ggggcgctgg tacatactgc tgataattat ccgcttctgt
    18001 taaccgtcgc ttttctctgt tcggcggtgc tggcgatgtt cggctacttt ttagtgcgca
    18061 aatacgcgcc ggaagcaggt ggttcgggga tcccggaaat tgaaggggcg ctggaagatc
    18121 aacgtcccgt tcgctggtgg cgtgtattgc cggtgaaatt ctttggcggg ctggggacac
    18181 tcggcggagg catggtgcta gggcgcgaag ggccaaccgt gcagatcggc ggtaatatcg
    18241 ggcggatggt actcgatgtt ttccgcctga aaggtgatga agctcgccat acgctgttgg
    18301 caaccggtgc ggcggcgggt ctggcggcgg cctttaacgc gccgctggcg ggtattttgt
    18361 ttattatcga agagatgcgt ccgcagtttc gctatacgtt aatttcgatt aaagcggtat
    18421 ttattggcgt cattatgtcg accattatgt accggatttt taatcatgaa gttgcgttga
    18481 ttgacgtcgg taaactttct gacgcgccgc ttaatacgct gtggctttat ctgatcctcg
    18541 gtattatttt tggcattttc ggccctattt ttaataaatg ggtgctgggg atgcaggatt
    18601 tgctgcaccg tgtgcacggc ggcaatatta ccaaatgggt actgatgggt ggtgcgattg
    18661 gcggtctgtg tggattgctg gggtttgttg caccagcaac gtcgggcggc ggttttaacc
    18721 tgattcctat cgctaccgcg gggaatttca gcatgggaat gctggtgttt atcttcgtcg
    18781 cgcgggtcat taccacctta ctctgcttct cttccggcgc gccgggcggt atttttgccc
    18841 cgatgctggc gctgggtact gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt
    18901 ttccgcaata tcaccttgag gcggggacgt ttgctattgc cggaatgggg gcgttattgg
    18961 cggcatctat tcgcgcgccg ttaacgggga tcattctggt tctggagatg accgataact
    19021 accagctcat tttgccaatg attattaccg gtcttggcgc aacactatta gcgcaattta
    19081 ccggcgggaa accgctatac tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg
    19141 agcaactggc gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg aaataccagg
    19201 gtattagata atggcgatta ttattgggtt agaatttgcc caattgccga tgtcgtttgg
    19261 agcaaaatat gagtgatgac gtagcactgc cgctggagtt taccgacgca gcagccaaca
    19321 aagttaaaag cctgatcgct gacgaagata acccgaatct gaaattacgc gtgtatatca
    19381 ccggtggcgg ttgcagcggc ttccagtatg gtttcacctt tgatgatcag gtgaacgaag
    19441 gcgatatgac catcgaaaaa cagggcgttg gcctggtggt tgatccgatg agcctgcaat
    19501 atctggtcgg cggttccgtt gactataccg aaggactgga aggttctcgt ttcatcgtga
    19561 ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc ctttagtatc taatctgttg
    19621 ttcatgtgcc ggataaacta tccggcacat cttctctctt caacgcccat tctcatccag
    19681 cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc agccgaatca atagcgtcac
    19741 gaccatgccc atcatactgg ctgtttccag tggtacggaa aatgtgtaat aagccgtagc
    19801 gtggacaata ccgccgataa tacaggcagt tgcgtagatt tctgtacgta aaatcatggg
    19861 gatttcgcgg gccagaacat cacgaatgat cccgccgcca acgccagtaa tgacgcccat
    19921 acaaaccgcg attaacggac cggcttccgc attaaaggct ttattcacgc caatgccgac
    19981 aaacaccgcc agaccaacgg cgtccagcac cggcaacatc cattttggta agcgtcttgg
    20041 ctggcgcacc agcacgatgg tcagcatgct ggttaccatt gcaacgacca gatcggtggg
    20101 atctttcacc caaaataccg ggccgtgatc cagcgccatg tcgcgaattg tcccgccgcc
    20161 tacagcggta accacgccca gtaccagaac accaaaaggg tccatacgca attttccggc
    20221 taacaaaacg ccggagatgg caaataccgc tgtgccgact atatccagcc aatagacgag
    20281 cattgttcaa tccccactga gcacctaaaa aggtcaggcg ctaatctacc tgtgaaagcg
    20341 cattacagag ctgttgtgca gcgaggataa tacgtgggct tgcacgttca aaccagtcac
    20401 tcgtgagagg aataacggga attttgagct gttcacccca gtattgttcg attttaggaa
    20461 tttggtccgg tccgcctgta atgacaatcg cctgtggcga gcgtgctaac acctgttcgc
    20521 tgctaacttg cggccaggga acccggctgt ctttaaagat gttttctccg ccacaaactt
    20581 cgagtacctg gttctgaatc gactcttttc cactggtaaa tggcggatta atgccgaatt
    20641 gcagaaaaac acgtttttta ggtttatcag catattgcgc tttcaattgc gcgtactgat
    20701 ccagcaggga ttgcgcggct tgttcggcct tatccggttg cggactccag ggggccagtt
    20761 gacgtaacgc attggcaatt tgttcaatgc ttgtcgcatc gacccacatc acttttattc
    20821 ccagcgaagc cagctggtca acctgccgct cggcattacc tccacgccag gcaatcacca
    20881 gatcgggttt cagcgcgaca atgcgttcca gattcatccc ctgccaggtg gaaacctgct
    20941 caatcttttg cgcttgtgga ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc
    21001 cggcggcaaa ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc ggtgccgcgt
    21061 tgagccacag tggcgcgaga aaagacaggg cgaccagcgc cctgaacagt gacttagcca
    21121 tgtgccagtt tctgcaccag tgactcaacc atcaggctgg actgtttagc ggcaacagcc
    21181 aggaactcat cgaagctaag atgagactgt tgatcggcca cgtcggagat ggcgcgtacg
    21241 acaacaaacg ggacgttgaa attgtggcag acatgggcga ttgccgtcgc ttccatctct
    21301 acagcaatgg cctgtgggaa gttgtggcgg attttcgcca gaccaacaga accgttgatg
    21361 aaagcgtcgc cgctaacaat caggccacgt acagcgttaa gattcagttc ggcaatgcag
    21421 gcctcagcgg cagcgatcag tttatcgtca gctttaaagc ctgccggaca gcctggtaac
    21481 tgaccgtatt cataaccaaa tgccgtgaca tccgcgtcgt gataacgtgc ttcgtccgag
    21541 acaacgatat cgcccacttt caacgttggt gccaggccac cggcagaacc ggtgttaata
    21601 atcacatctg gcttgcagtg ttccaacagc aaagtggcac ccagcgccgc agcgacttta
    21661 ccgatgcccg atttcagaag cgcaacctcg gttccattca gttggccggt atagatttcg
    21721 caaccgccga gactgatagt ttgacggttt tcgattttgt cacgcagcag cgtaacttct
    21781 tcttccattg caccaatgat gccgattttc atagatttac tcgcgataag cccgatttga
    21841 aggcatagtt taccatgcgc ttacggggaa gcgtatttct cacgcgggag aggacatggc
    21901 acagattgat ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac cgcagggcgt
    21961 taaaaccgaa catgagatcc tgcggatctt cgagagcgat cgcgggcgta tcatcaactc
    22021 tccggcaatt cgtcgtctgc aacaaaagac ccaggttttt ccactggagc gcaatgccgc
    22081 cgtgcgcacg cgtcttaccc actcgatgga agtccagcag gtggggcgct acatcgccaa
    22141 agaaatttta agccgtctga aagagcttaa attgctggaa gcatacggcc tggatgaact
    22201 gaccggacct tttgaaagca ttgttgagat gtcatgcctg atgcacgata tcggcaatcc
    22261 gccgtttggt cattttggcg aagcggcgat aaatgactgg tttcgccagc gtttataccc
    22321 ggaagatgcc gaaagccagc ctctgactga cgatcgctgc agcgtggcgg cactacgttt
    22381 acgggacggg gaagaaccgc ttaacgagct gcggcgcaag attcgtcagg acttatgtca
    22441 ttttgagggg aatgcacaag gcattcgtct ggtgcataca ttgatgcgga tgaatctcac
    22501 ctgggcgcag gttggcggta ttttaaaata tacccgtcca gcgtggtggc gtggcgaaac
    22561 gcctgagaca catcactatt taatgaaaaa gccaggttat tatctttctg aagaagccta
    22621 tattgcccgg ttgcgtaaag aacttaattt ggcgctttac agtcgttttc cattaacgtg
    22681 gattatggaa gctgccgacg acatctccta ttgtgtggca gatcttgaag atgcggtaga
    22741 gaaaagaata tttaccgttg agcagcttta tcatcatttg cacgaggcgt ggggccagca
    22801 tgagaaaggt tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa
    22861 tagtttaagc cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa acaccctaaa
    22921 taaactggta ccctacgcgg cacaacgatt tattgataat ctgcctgcga ttttcgccgg
    22981 aacgtttaat catgcattat tggaagatgc cagcgaatgc agcgatcttc ttaagctata
    23041 taaaaatgtc gctgtaaaac atgtgtttag ccatccagat gtcgagcagc ttgaattgca
    23101 gggctatcgg gtcattagcg gattattaga gatttatcgc cctttattaa gcctgtcgtt
    23161 atcagacttt actgaactgg tagaaaaaga acgggtgaaa cgtttcccta ttgaatcgcg
    23221 cttattccac aaactctcga cgcgccatcg gctggcctat gtcgaagctg tcagtaaatt
    23281 accgtcagat tctcctgagt ttccgctatg ggaatattat taccgttgcc gcctgctgca
    23341 ggattatatc agcggtatga ccgacctcta tgcgtgggat gaataccgac gtctgatggc
    23401 cgtagaacaa taaccaggct tttgtaaaga cgaacaataa atttttacct tttgcagaaa
    23461 ctttagttcg gaacttcgcg ctataaaacg aatctgaaga acacagcaat tttgcgttat
    23521 ctgttaatcg agactgaaat acatgaaaaa aaccacatta gcactgagtg cactggctct
    23581 gagtttaggt ttggcgttat ctccgctctc tgcaacggcg gctgagactt cttcagcaac
    23641 gacagcccag cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt
    23701 cagcattaac gtagaaggta gcacaaccgt taatacgccg cgtatgccgc gtaatttcca
    23761 gcagttcttc ggtgatgatt ctccgttctg ccaggaaggt tctccgttcc agagctctcc
    23821 gttctgccag gggggccagg gcggtaatgg cggcggccag caacagaaat tcatggcgct
    23881 gggttccggc gtcatcattg atgccgataa aggctatgtc gtcaccaaca accacgttgt
    23941 tgataacgcg acggtcatta aagttcaact gagcgatggc cgtaagttcg acgcgaagat
    24001 ggttggcaaa gatccgcgct ctgatatcgc gctgatccag atccagaacc cgaaaaacct
    24061 gaccgcaatt aagatggcgg attctgatgc actgcgcgtg ggtgattaca ccgtagcgat
    24121 tggtaacccg tttggtttgg gcgagacggt aacttccggg attgtctctg cgttggggcg
    24181 tagcggcctg aatgccgaaa actacgaaaa cttcatccag accgatgcag cgatcaaccg
    24241 tggtaactcc ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta tcaacaccgc
    24301 gatcctcgca ccggacggcg gcaacatcgg tatcggtttt gctatcccga gtaacatggt
    24361 gaaaaacctg acctcgcaga tggtggaata cggccaggtg aaacgcggtg agctgggtat
    24421 tatggggact gagctgaact ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg
    24481 tgctttcgta agccaggttc tgcctaactc ctccgctgca aaagcgggca ttaaagcagg
    24541 tgatgtgatc acctcactga acggtaagcc aatcagcagc tttgccgcac tgcgtgctca
    24601 agtgggtact atgccggtgg gcagcaaact gaccctgggc ttgctgcgcg acggtaagca
    24661 ggtcaacgtg aatctggaac tgcagcagag cagccagaat caggttgatt ccagctccat
    24721 cttcaacggc attgaaggcg ctgagatgag caacaaaggc aaagatcagg gcgtggtagt
    24781 gaacaacgtg aaaacgggca ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat
    24841 tattggcgcg aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag
    24901 caaaccgtct gtgctggcac tgaacattca gcgcggcgac agcaccatct acctgttaat
    24961 gcagtaatct ccctcaaccc cttcctgaaa acgggaaggg gttctcctta caatctgtga
    25021 acttcaccac aactccatac atcttcatca tcctttaggc atttgcacaa tgccgtacgt
    25081 tacgtacttc cttatgctaa gccgtgcata acggaggact tatggctggc tggcatcttg
    25141 ataccaaaat ggcgcaggat atcgtggcac gtaccatgcg catcatcgat accaatatca
    25201 acgtaatgga tgcccgcggg cgaattatcg gcagcggcga tcgtgagcgt attggtgaat
    25261 tgcacgaagg agcattgctg gtactttcac agggacgagt cgtcgatatt gatgacgcgg
    25321 tggcacgtca tctgcacggt gtgcggcagg ggattaatct accgttacgg ctggaaggtg
    25381 aaattgtcgg cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac
    25441 tggtctgcat gacggctgaa atgatgctgg aacagtcgcg gttgatgcac ttgttggcgc
    25501 aggatagccg tttgcgggag gaactggtga tgaacctgat tcaggcagag gagaatactc
    25561 ccgcacttac tgaatgggcg caacggctgg ggatcgatct caatcaaccg cgagtggtgg
    25621 ctattgttga ggtcgacagc ggtcagcttg gtgtggacag cgcaatggcg gagttacaac
    25681 aactgcaaaa cgcgctgact acgcccgaac gtaataatct ggtggcgatt gtctcgctaa
    25741 ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg gcgctgggat gcagaagatc
    25801 atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga gtacggccag ctgcgttttc
    25861 gcgtttcact gggcaactat tttaccggtc ctggcagtat tgcccgatcc tatcgtacgg
    25921 cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc tatttttatc
    25981 aggatctgat gttacctgtg ttactcgaca gtttgcgtgg cgactggcag gccaacgaac
    26041 tggcgcgacc gctggcgcgg ctgaaagcga tggacaataa cggcttgctg cgacgaacgc
    26101 tggcggcgtg gtttcgtcac aatgtgcaac cgctggcaac gtcaaaggcg ttgtttattc
    26161 atcgtaatac cctggagtat cggcttaatc gtatatcgga actgaccggg cttgatttgg
    26221 gcaattttga tgacaggttg ctgctgtatg tggcgttaca actggatgaa gagcggtagg
    26281 ttatgcgtta aggttgagga ggcgctggct cattcccctc agcccgaagg ggagagggga
    26341 atgcccgagt taaccgtcaa ttcgggatta tttacgcgtt agtttttcca gatcggcttc
    26401 aatctcgctg atcttattgg ttacgactga ctccaggtga cgtaagtcgt cgaggatctt
    26461 acgcttaaga tcaacttcgc tgcggtcacg ctgacagatt tgatcaagct catcaatgat
    26521 ataccgtaga ttcgggctga tttcctggac ttctttataa ccctgaccca caccatcagc
    26581 gacgaccgtt ttacgctgac gcggatattt aaacttaacg ctcttggcga aaaactcgcc
    26641 tttgtctttc tggaaataga ttttcagaat atcgttgttg gcttcctgcc ggaggctgta
    26701 acgatcaatt tcttcaggat tggtaatacc cagacttttc agattgtcgt acatagcgtt
    26761 acctcaaaat gagtcagtaa attgtgctta ttttagcatt tggcctggcc cgcccggctc
    26821 gactgtgatc gcagaaagcc tttttcttcc agacttctat catggcgtaa aaatcaaaaa
    26881 attacctgct ttattgtggc gatattccat gggatggatt agcacattta tcttcgtttt
    26941 gcgaattaca tgacagtccg aaacaagatg aaaaagtatt taaaaaaggg ggaggaagaa
    27001 tgagtaggac gaattattct gaaccagaaa gcaatcgggc agcgtcgtgc tgcccgtgtg
    27061 catactttta gtcgatggta cgcaacagtt cgttaatgcc gactttgccg cgagttttcg
    27121 cgtcaacttt cttaacgata accgcacagt aaagactgta tttgccatct ttcgacggca
    27181 gattgcctga aacaaccaca gaccccgccg gaacgcgtcc gtagtggact tcgccagttt
    27241 cgcgatcata gatcttggtg ctttgcccaa tgtaaacacc cattgagatc accgaacctt
    27301 cttctacgat gacgccttca acgatttctg aacgcgcgcc gatgaagcag ttatcttcaa
    27361 taatggtcgg gttagcctgc agcggttcca gcacaccgcc gatgcccacg ccaccggaaa
    27421 ggtggacgtt tttaccaatc tgcgcacaag aaccgacggt cgcccaggta tcgaccatgg
    27481 tgccttcatc aacatatgcg ccgatgttga cgtaagacgg catcagcacg gtgttacggg
    27541 caataaacgc accctgacgt accgccgcag gtggtacaac gcggaagcct tctttctgga
    27601 aacgtgcttc gtcgtagtcg gcgaatttca tcggcacttt gtcgaagtag cggctttctg
    27661 ccccttcgat cacctgatta tcattaatac ggaaagagag cagcaccgct tttttcaacc
    27721 actgatgcgt cacccactga ccgtcaattt tttccgctac acgcagtgcg ccggaatcca
    27781 gcagggcgat cacctgattt accgcttcgc gggtaacggt gtctgcattg gctggcgtga
    27841 tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg taactgctgc attgttaaac
    27901 tcttttcata tcagtaaaca catcaccctt tatcgtttgg attgagggcc tctgtcaacc
    27961 gctgatgcac ttcctgctgc aactcgttat taagtgcacg ccggtcagcg gtggcaatta
    28021 tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg ggcaccatga agcgaaattc
    28081 ccagatcggc aaaaattttc ccgactcgcg ccagcagccc aggttggtcg agggcgatca
    28141 gttcgaggaa cgatttgcgg tcggtatggg ttggcaaaaa cgtcacttcg gtttcaacag
    28201 taaaatggcg caatttggcg ggttggcgac ggggctgcgg tggctgccag ctactttgtg
    28261 tcagtacttg ctccagacca aaccgaataa cctcatggcg atctgcggac agcgggctgc
    28321 catcgggttc cagcacgata aaggtatcca tcgccatacc gtcgcgagtg gtgaatattt
    28381 gtgcgtcgtg aacgcttaag ttgcgtctat ctaactcggc acaaacagca gcaaacaagt
    28441 aagggcggtc cgggctccag ataaaaatct cggtgcctcc acgcgtagcc tgcgggctaa
    28501 gcaataccag cggtttgctt aaatcatgct gtaataaatg gcgcgcatgc caggccagtt
    28561 gatttgggct atggcggaca aaatagttag cacgacagcg tgaccaaatt tggtgcagcg
    28621 cctcttcgtc gatgttatcc atgcgcagca gtgccagcgc ctggagttgg tgatggcgaa
    28681 cccgttcgcg catatccggc gtgttttgca ttccgcgtcg tagctgtttt tcggtggcga
    28741 aatagagttc gcgcaacaga ctttgcttcc agctattcca cagcgtttcg ttggtggcgc
    28801 aaatgtcagc cactgtcagg cataccagat agcgcagacg attttccgtt tgaacttctt
    28861 cggcaaactg cttgatgact tccgggtcct gaatatcgcg acgctgagct gtcaccgaca
    28921 tcagcaagtg ttggcgaacc agccaggcga ccagctgtgt ttcgcgtgag ttcagcccgt
    28981 ggagttcggc aaaatgcact acatcctgag caccgagaat ggaatggtcg ccgccgcgtc
    29041 ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag ctcagttgac ggtaggcgcg
    29101 gccagacgtc cacacacaac ggatggcgct ggcgcgtttc ttcactggca aaactctcca
    29161 gtttcagcat cacgcggata gtatgttcat ccaccgtgta ggcgtggaac agatcaaact
    29221 gcatctgccc gacgatatgt gaccattgcg gcatatacgc gccgagtacg ctatggcgat
    29281 gcattggcaa tagcccgcgc cgcaccgctc cggggtgacg cagaatgctc aaaaacagtt
    29341 ttcgtgcttc cggaatatta cacagcggtt gttgcagatg gcgacgggca tggcgtaact
    29401 ggcgcagtgt ggtggagtaa atgccggtga tcgcactgtt gcgcaccatg gtgtagaaca
    29461 tacgcaagat ggcttccggc tggcgcataa atagtgtttc atcacgcagg tcgattagcg
    29521 taccgcgtag ctgaaactca tcgtcgattg gacgtggttt ttcgtcggcg ggaagggcga
    29581 ggatggcttc atcgaacagt tgcagcagca tctggttgag ttcactgacg cggcgtgtca
    29641 cgcggaagta atccttcatc atccgctcga ccggttcgtt accttcgcca ctgtaattca
    29701 gacgctgggc gacgctaagc tggcgatcga ataacaggcg attatcgtaa cggctgacga
    29761 ccagatgcag ggcaaagcga atacgccaca atatatgcag acattcgttt aattccgccc
    29821 gctccgctga ggttaagaag ccaaacccaa ccatttcatc cagcgatgtt gcgccaaaat
    29881 gacggcgggc cacccattgc agagtgtgga tatcgcgcaa gccgccaggg ctacttttga
    29941 tgtctggttc aagattgtag ctggtgccgt ggtaacgctg atggcgctgg ttctgttctt
    30001 caactttcgc cgcgtagaac ttgtcggaag gccagaatcc ttcgctgaaa atatgttttt
    30061 gcagttcaag aaatagcgca acatcgccaa ttaataagcg ggattcgatc aaattggtgg
    30121 cgacggttaa atccgataac ccttccagca tgcactcttc aagcgtgcgc acactatgac
    30181 cgacttccag ctttacatcc cagagcagcg ttaacagctc gcccactttt tgcgcctgat
    30241 cgtccgggag ctttttacgg cttaaaatca gtaaatcgat gtcagaaagt ggatgcagct
    30301 cgccacgacc gtagccaccg acggcgacca atgccaggtc ggcaatctgg ctgaatcccg
    30361 cttcaatcca taatcgttgc aggagctggt cgataaactc ggtgcgcgcc tcaatcaact
    30421 gttctgcaga gatcccattg tcaaaggcat cacccagcca acgctggaaa gtatcgatat
    30481 gggcttttat cccaccgacg gttaattcat cacggggcca gacgcatgga ttttgcggtt
    30541 gaccgggcag ggtggggaga gcggtgtttg cgtactgttc tggaagggta ttcattgtgc
    30601 gccacccata agattaaatt atcgcattaa aaaagccggc attcgccggc ttcattttat
    30661 tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc gtagtgtcag aatttcgcag
    30721 ccgttatcag tcaccacaat agtatgctca tattgtgcag acaagctgcg atctttggtt
    30781 tttaccgtcc agccatcttt catggtgcgg atctcttttt tacccgcgtt gaccattggc
    30841 tcgatggtga acgtcatccc aggtttcagt acgacgttgg tttcacggga gtcatagtgc
    30901 agcacctgcg gttcttcgtg gaagccgcga ccaataccgt gtccgcaata ttcacgaacg
    30961 acggagaagc cttctgcttc gacaaatttc tgaatcgccg caccgatttc gcgcagatta
    31021 atgcctggtt ttaccatgcg tagcgccagg tacaggcttt cttgcgtgat acggcacaga
    31081 cgttcgccca tgatggtcgg cttaccgacg ataaacattt tcgaggtatc gccgtggaaa
    31141 ccatctttga ttacggtgac atcaatgtta acgatatcgc catctttcag cagcttcgca
    31201 tcgtccggga taccgtggca caccacttca ttaattgaga tgcaaacgga tttcggataa
    31261 ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat taacaatgta atcattacag
    31321 atgcgatcca gctcgccggt gctgacgccc ggtttaacat acggttcgat catctccagc
    31381 acttcggcag ccagtcggcc agcgacgcgc attttttcga tatcttctgg ggtcttgatt
    31441 gagatagcca ttaattctgt ccatcagcgt cggtgatacc gacaatatat atgtaagtgc
    31501 cgtcaatggt atcacaccct ggcaaattga gaatcattct gaatttcgcc aaacgtgcca
    31561 ctgaaggttt tctataatag aaaattcgac gtctgatgct gtacacagcg ccaacaatta
    31621 ttggtgtcca cggcgtattt gtggtataaa gcgcgccgga cttccgatcc atttcgtata
    31681 cacagactgg acggaagcga caatctcact ttgtgtaaca acacacacgt atcggcacat
    31741 attccggggt gccctttggg gtcggtaata tgggatacgt ggaggcataa ccccaacttt
    31801 tatatagagg ttttaatcat ggcaactgtt tccatgcgcg acatgctcaa ggctggtgtt
    31861 cacttcggtc accagacccg ttactggaac ccgaaaatga agccgttcat cttcggtgcg
    31921 cgtaacaaag ttcacatcat caaccttgag aaaactgtac cgatgttcaa cgaagctctg
    31981 gctgaactga acaagattgc ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc
    32041 gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg accagttctt cgtgaaccat
    32101 cgctggctgg gcggtatgct gactaactgg aaaaccgttc gtcagtccat caaacgtctg
    32161 aaagacctgg aaactcagtc tcaggacggt actttcgaca agctgaccaa gaaagaagcg
    32221 ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc tgggcggtat caaagacatg
    32281 ggcggtctgc cggacgctct gtttgtaatc gatgctgacc acgaacacat tgctatcaaa
    32341 gaagcaaaca acctgggtat tccggtattt gctatcgttg ataccaactc tgatccggac
    32401 ggtgttgact tcgttatccc gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg
    32461 ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg atctggcttc ccaggcggaa
    32521 gaaagcttcg tagaagctga gtaataaggc acgctcgtac gagcccttat agaccaggta
    32581 gtacacgttt ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg
    32641 ttcaatgccg ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc
    32701 tggtaaaaga gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga
    32761 ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta
    32821 aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg
    32881 gcaactacgg catcattctg gaagttaact gccagactga cttcgttgca aaagactctg
    32941 gtttccaggc gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg
    33001 ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg
    33061 aaaacatcaa cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc
    33121 acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac
    33181 acatcgctat gcacgttgct gcaagcaagc cagaattcat caaaccggaa gacgtatccg
    33241 ctgaagtggt agagaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc
    33301 cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt
    33361 ctctgaccgg tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag
    33421 agcataacgc tgaagtgact ggcttcatcc gcttcgaagt aggtgaaggc atcgagaaag
    33481 ttgagactga ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcgaaaa
    33541 ggagccgcct gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg
    33601 gggctgcgct gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca
    33661 gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa
    33721 gttgagtggc gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga
    33781 tcgcatggct caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat
    33841 tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt
    33901 gggcgaccac atggggatgc tggcgaccgt aatgaacggc ctggcaatgc gtgatgcact
    33961 gcaccgcgcc tatgtgaacg ctcgcctgat gtccgctatt ccattgaatg gcgtgtgcga
    34021 cagctacagc tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc
    34081 cgccggtaca ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga
    34141 aattgaagcc gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ctgctgatcc
    34201 ggcgaaagat ccaactgcaa ccatgtacga gcaattgact tacagcgaag tgctggaaaa
    34261 agagctgaaa gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat
    34321 tcgtgttttc aacatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga
    34381 agggacttta atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa
    34441 gtatcgcggg ggatcttacc cgtaatatgt ttaatcaggg ctatacttag cacacttcca
    34501 ctgtgtgtga ctgtctggtc tgactgagac aagttttcaa ggattcgtaa cgtgattagc
    34561 gatatcagaa aagatgctga agtacgcatg gacaaatgcg tagaagcgtt caaaacccaa
    34621 atcagcaaaa tacgcacggg tcgtgcttct cccagcctgc tggatggcat tgtcgtggaa
    34681 tattacggca cgccgacgcc gctgcgtcag ctggcaagcg taacggtaga agattcccgt
    34741 actctgaaaa tcaacgtgtt tgatcgttca atgtctccgg ccgttgaaaa agcgattatg
    34801 gcgtccgatc ttggcctgaa cccgaactct gcgggtagcg acatccgtgt tccgctgccg
    34861 ccgctgacgg aagaacgtcg taaagatctg accaaaatcg ttcgtggtga agcagaacaa
    34921 gcgcgtgttg cagtacgtaa cgtgcgtcgt gacgcgaacg acaaagtgaa agcactgttg
    34981 aaagataaag agatcagcga agacgacgat cgccgttctc aggacgatgt acagaaactg
    35041 actgatgctg caatcaagaa aattgaagcg gcgctggcag acaaagaagc agaactgatg
    35101 cagttctgat ttcttgaacg acaaaaacgc cgctcagtag atccttgcgg atcggctggc
    35161 ggcgttttgc tttttattct gtctcaactc tggatgtttc atgaagcaac tcaccattct
    35221 gggctcgacc ggctcgattg gttgcagcac gctggacgtg gtgcgccata atcccgaaca
    35281 cttccgcgta gttgcgctgg tggcaggcaa aaatgtcact cgcatggtag aacagtgcct
    35341 ggaattctct ccccgctatg ccgtaatgga cgatgaagcg agtgcgaaac ttcttaaaac
    35401 gatgctacag caacagggta gccgcaccga agtcttaagt gggcaacaag ccgcttgcga
    35461 tatggcagcg cttgaggatg ttgatcaggt gatggcagcc attgttggcg ctgctgggct
    35521 gttacctacg cttgctgcga tccgcgcggg taaaaccatt ttgctggcca ataaagaatc
    35581 actggttacc tgcggacgtc tgtttatgga cgccgtaaag cagagcaaag cgcaattgtt
    35641 accggtcgat agcgaacata acgccatttt tcagagttta ccgcaaccta tccagcataa
    35701 tctgggatac gctgaccttg agcaaaatgg cgtggtgtcc attttactta ccgggtctgg
    35761 tggccctttc cgtgagacgc cattgcgcga tttggcaaca atgacgcccg atcaagcctg
    35821 ccgtcatccg aactggtcga tgggacgtaa aatttctgtc gattcggcta ccatgatgaa
    35881 caaaggtctg gaatacattg aagcgcgttg gctgtttaac gccagcgcca gccagatgga
    35941 agtgctgatt cacccgcagt cagtgattca ctcaatggtg cgctatcagg acggcagtgt
    36001 tctggcgcag ctgggggaac cggatatgcg tacgccaatt gcccacacca tggcatggcc
    36061 gaatcgcgtg aactctggcg tgaagccgct cgatttttgc aaactaagtg cgttgacatt
    36121 tgccgcaccg gattatgatc gttatccatg cctgaaactg gcgatggagg cgttcgaaca
    36181 aggccaggca gcgacgacag cattgaatgc cgcaaacgaa atcaccgttg ctgcttttct
    36241 tgcgcaacaa atccgcttta cggatatcgc tgcgttgaat ttatccgtac tggaaaaaat
    36301 ggatatgcgc gaaccacaat gtgtggacga tgtgttatct gttgatgcga acgcgcgtga
    36361 agtcgccaga aaagaggtga tgcgtctcgc aagctgagga taatccggct acagagagtc
    36421 gcgctatttg ttagcgtagg gcttcagtga tatagtctgc gccatctgat cgtaagtagt
    36481 tggctttata aggtcaggta tgccgtggtt ttacacggct tttttttgta taggcttcag
    36541 tattcctgag taccgtaaac cctgtcaggg aataaaaaac gcgtgatgtt gtctgctact
    36601 caaccactta gcgaaaaatt gccagcgcat ggctgccgtc atgttgcgat cattatggac
    36661 ggcaatggtc gctgggcaaa aaagcaaggg aagattcgtg cctttgggca taaagccggg
    36721 gcaaaatccg tccgccgggc tgtctctttt gcggccaaca acggtattga ggcgttaacg
    36781 ctgtatgcct ttagtagtga aaactggaac cgaccagcgc aggaagtcag tgcgttaatg
    36841 gaactgtttg tgtgggcgct cgatagcgaa gtaaaaagtc tgcaccgaca taacgtgcgt
    36901 ctgcgtatta ttggcgatac cagtcgcttt aactcgcgtt tgcaagaacg tattcgtaaa
    36961 tctgaagcgc taacagccgg gaataccggt ctgacgctga atattgcggc gaactacggt
    37021 ggacgttggg atatagtcca gggagtcagg caactggctg aaaaggtgca gcaaggaaac
    37081 ctgcaaccag atcagataga tgaagagatg ctaaaccagc atgtctgtat gcatgaactg
    37141 gcccctgtag atttagtaat taggactggg ggggagcatc gcattagtaa ctttttgctt
    37201 tggcaaattg cctatgccga actttacttt acagatgttc tctggcccga tttcgatgaa
    37261 caagactttg aaggggcgtt aaatgccttt gctaatcgag agcgtcgttt cggcggcacc
    37321 gagcccggtg atgaaacagc ctgatggggg tcgcttttgc tgaagtatcg cctgatatct
    37381 gcttttgtgt taatacccgt cgtcatcgcg gcgttgtttc tgttgccgcc ggtggggttc
    37441 gccattgtaa cgctggtggt ctgcatgctg gcagcgtggg aatggggaca gcttagcggt
    37501 tttaccactc gttcgcagcg agtatggttg gcggtgttat gcgggttatt gttggcgctg
    37561 atgctttttc tgttgccgga atatcaccga aatattcatc aaccgctggt tgaaatctca
    37621 ctttgggctt cgctgggttg gtggattgtc gcgctattgc tggtgctgtt ttacccaggt
    37681 tccgcagcaa tctggcgtaa ctctaaaaca ttgcgcctta tttttggcgt gctaaccatt
    37741 gttcccttct tctggggcat gctggcgtta cgggcctggc actacgacga gaatcattac
    37801 agtggcgcaa tatggctgct ctatgtcatg atcctggtat ggggcgctga ctctggcgca
    37861 tatatgtttg gcaaattgtt tggtaaacat aagctggcac cgaaggtttc tccgggtaaa
    37921 acctggcaag gctttatagg tggactcgcg accgcagcag taatctcatg gggttatggc
    37981 atgtgggcga atctcgacgt cgctcccgtc accttactca tttgctctat tgtcgcagcg
    38041 ttagcctcag tgctcggcga tctgaccgag agtatgttta agcgtgaagc aggaattaag
    38101 gacagcggtc atttaattcc aggacacggt ggtattttag atcgtattga tagcctgacg
    38161 gctgcggtac cggtctttgc ttgcttgttg ttactggtat tcaggacgct ttaacggaag
    38221 gtaatatgct gagttttctc tgggatttgg cttcgttcat cgttgcactg ggtgtactta
    38281 tcaccgtgca tgaatttggt catttctggg ttgcccggcg ttgtggtgtt cgcgttgagc
    38341 gtttctcaat agggtttggt aaggcgctct ggcggcgaac tgataagctc ggcaccgaat
    38401 atgttatcgc cctgatcccg ttgggcggtt atgtcaaaat gctggatgag cgcgcagaac
    38461 cggtcgttcc ggaactccgc caccatgcct tcaataataa atctgtcggc caacgagcgg
    38521 cgattattgc cgcaggtccg gttgcaaact tcatttttgc tatctttgcc tactggctgg
    38581 tttttattat tggtgtgcct ggcgtacgtc cggtggttgg cgaaatagca gccaattcga
    38641 tagctgcgga agcacaaatt gcaccaggta cggaactaaa agccgtagat ggtatcgaaa
    38701 cgcctgattg ggatgccgtg cgtttgcagt tggtcgataa aattggcgat gaaagcacca
    38761 ccattacagt agcgccattt ggcagcgacc aacggcggga tgtaaagctc gatttacgtc
    38821 actgggcgtt tgagcctgat aaagaagatc cggtatcttc gctggggatt cgtcctcgtg
    38881 ggccgcaaat tgaacctgta ctggaaaatg tgcagccaaa ctcggcggca agcaaggcag
    38941 gtttgcaagc aggcgacagg atcgttaaag tcgatggtca gcccttaacg cagtgggtga
    39001 cctttgtgat gcttgtccgg gataacccgg gtaaatcctt agcgttagaa atcgaaaggc
    39061 aggggagtcc cttgtctttg acattaatcc cggagagtaa accgggtaat ggtaaagcga
    39121 ttggttttgt cggtattgag ccgaaagtca ttcctttgcc agatgagtat aaagttgtac
    39181 gccagtatgg gccgttcaac gccatcgtcg aagccacgga caaaacgtgg cagctgatga
    39241 agctgacggt cagtatgctg ggaaaattga tcaccggtga tgtgaaactg aacaacctca
    39301 gtgggccgat ctctatcgcc aagggggctg ggatgacagc ggaactcggg gtagtttatt
    39361 acctgccgtt tcttgcgctt attagcgtga acttagggat aattaacctg tttccgttgc
    39421 ccgtacttga cggggggcat ctgctgttcc ttgcgatcga aaagatcaag ggcggaccgg
    39481 tatccgagcg ggttcaagac ttttgttatc gcattggctc gattctgctg gtgctgttaa
    39541 tggggcttgc acttttcaat gatttctctc ggttatgaga gttagttagg aagaacgcat
    39601 aataacgatg gcgatgaaaa agttgctcat agcgtcgctg ctgtttagca gcgccaccgt
    39661 atacggtgct gaagggttcg tagtgaaaga tattcatttc gaaggccttc agcgtgtcgc
    39721 cgttggtgcg gccctcctca gtatgccggt gcgcacaggc gacacggtta atgatgaaga
    39781 tatcagtaat accattcgcg ctctgtttgc taccggcaac tttgaggatg ttcgcgtcct
    39841 tcgtgatggt gatacccttc tggttcaggt aaaagaacgt ccgaccattg ccagcattac
    39901 tttctccggt aacaaatcgg tgaaagatga catgctgaag caaaacctcg aggcttctgg
    39961 tgtgcgtgtg ggcgaatccc tcgatcgcac caccattgcc gatatcgaga aaggtctgga
    40021 agacttctac tacagcgtcg gtaaatatag cgccagcgta aaagctgtcg tgaccccgct
    40081 gccgcgcaac cgtgttgacc taaaactggt gttccaggaa ggtgtgtcag ctgaaatcca
    40141 gcaaattaac attgttggta accatgcttt caccaccgac gaactgatct ctcatttcca
    40201 actgcgtgac gaagtgccgt ggtggaacgt ggtaggcgat cgtaaatacc agaaacagaa
    40261 actggcgggc gaccttgaaa ccctgcgtag ttactatctg gatcgcggtt atgcccgttt
    40321 caacatcgac tctacccagg tcagtctgac gccagataaa aaaggtattt acgtcacggt
    40381 gaacatcacc gaaggcgatc agtacaagct ttctggcgtt caagtgagcg gcaaccttgc
    40441 cgggcactcc gctgaaattg agcagctgac taagatcgag ccgggcgagc tgtataacgg
    40501 caccaaagtg accaagatgg aagatgacat caaaaagctt ctcggtcgct atggttatgc
    40561 ctatccgcgc gtacagtcga tgcctgaaat taacgatgcc gacaaaaccg ttaaattacg
    40621 cgtgaacgtt gatgcgggta accgtttcta cgtgcgtaag atccgttttg aaggtaacga
    40681 tacctcgaaa gatgccgtcc tgcgtcgcga aatgcgtcag atggaaggtg catggctggg
    40741 aagcgatctg gtcgatcagg gtaaggagcg tctgaatcgt ctgggcttct ttgaaactgt
    40801 cgataccgat acccaacgtg ttccgggtag cccggaccag gtagatgtag tctacaaggt
    40861 gaaagaacgt aacaccggta gcttcaactt cgggattggt tacggtactg aaagtggcgt
    40921 gagcttccag gctggcgtgc aacaggataa ctggttaggt acaggttatg ctgttggtat
    40981 caacgggacc aaaaacgatt accagaccta tgctgaattg tcggtaacca acccgtactt
    41041 cactgtagat ggtgtaagcc tcggtggtcg tctcttctat aatgacttcc aggcagatga
    41101 cgccgacctg tccgactata ccaacaagag ttatggtaca gacgtgacgt tgggcttccc
    41161 gatcaacgaa tataactcgc tgcgtgcagg tctgggttat gtacataact ccctgtccaa
    41221 catgcagcct caggttgcga tgtggcgtta tctgtactct atgggtgaac atccgagcac
    41281 ctctgatcag gataacagct tcaaaacgga cgacttcacg ttcaactatg gttggaccta
    41341 taacaagctt gaccgtggtt acttcccgac agatggttca cgtgtcaacc tgaccggtaa
    41401 agtgaccatt cctggatcgg ataacgaata ctacaaagtg acgttagaca cggcgactta
    41461 tgtgccgatc gatgacgatc acaaatgggt tgttctgggg cgtacccgct ggggttatgg
    41521 tgatggttta ggcggcaaag agatgccgtt ctacgagaac ttctatgccg gtggttccag
    41581 caccgtgcgt ggcttccagt ccaataccat tggtccgaaa gcagtttact tcccgcatca
    41641 ggccagtaat tatgatccgg actatgatta cgaatgtgcg actcaggacg gcgcgaaaga
    41701 cctgtgtaaa tcggatgatg ctgtaggcgg taacgccatg gcggttgcca gcctcgagtt
    41761 catcaccccg acgccgttta ttagcgataa gtatgctaac tcggttcgta cttccttctt
    41821 ctgggatatg ggtaccgttt gggatacaaa ctgggattcc agccaatatt ctggatatcc
    41881 ggactatagt gatccaagca atatccgtat gtctgcgggt atcgcattac aatggatgtc
    41941 cccattgggg ccgttggtgt tctcctacgc ccagccgttc aaaaagtacg atggagacaa
    42001 ggcagaacag ttccagttta acatcggtaa aacctggtaa gtgttctcca caaaggaatg
    42061 tagtggtagt gtagcgatga ctttaggcga tcaatataag atcgccgggc cacgcaaaga
    42121 actgcaccct ccggtgcaaa tgggatggta aggagtttat tgtgaaaaag tggttattag
    42181 ctgcaggtct cggtttagca ctggcaacat ctgctcaggc ggctgacaaa attgcaatcg
    42241 tcaacatggg cagcctgttc cagcaggtag cgcagaaaac cggtgtttct aacacgctgg
    42301 aaaatgagtt caaaggccgt gccagcgaac tgcagcgtat ggaaaccgat ctgcaggcta
    42361 aaatgaaaaa gctgcagtcc atgaaagcgg gcagcgatcg cactaagctg gaaaaagacg
    42421 tgatggctca gcgccagact tttgctcaga aagcgcaggc ttttgagcag gatcgcgcac
    42481 gtcgttccaa cgaagaacgc ggcaaactgg ttactcgtat ccagactgct gtgaaatccg
    42541 ttgccaacag ccaggatatc gatctggttg ttgatgcaaa cgccgttgct tacaacagca
    42601 gcgatgtaaa agacatcact gccgacgtac tgaaacaggt taaataagta atgccttcaa
    42661 ttcgactggc tgatttagcg cagcagttgg atgcagaact acacggtgat ggcgatatcg
    42721 tcatcaccgg cgttgcgtcc atgcaatctg cacaaacagg tcacattacg ttcatggtta
    42781 acccaaaata ccgtgagcat ttaggcttgt gccaggcgtc cgcggttgtc atgacccagg
    42841 acgatcttcc tttcgcgaaa agtgccgcgc tggtagtgaa gaatccctac ctgacttacg
    42901 cgcgcatggc gcaaatttta gataccacgc cgcagcccgc gcagaacatt gcacccagtg
    42961 cggtgatcga cgcgacggcg aagctgggta acaacgtatc gattggcgct aacgcggtga
    43021 ttgagtccgg cgttgaactg ggcgataacg tgattatcgg tgccggttgc ttcgtaggta
    43081 aaaacagcaa aatcggtgca ggttcgcgtc tctgggcgaa cgtaaccatt taccatgaga
    43141 tccagatcgg tcagaattgc ctgatccagt ccggaacagt ggtaggcgca gacggctttg
    43201 gttatgccaa cgatcgtggt aactgggtga agatcccaca gattggtcgc gtaattattg
    43261 gcgatcgcgt ggagatcggt gcctgcacaa ccatcgatcg cggcgcgctg gatgacacta
    43321 ttattggcaa tggcgtgatc attgataacc agtgccagat tgcacataac gtcgtgattg
    43381 gcgacaatac ggcggttgcc ggtggcgtca ttatggcggg cagcctgaaa attggtcgtt
    43441 actgcatgat cggcggagcc agcgtaatca acgggcatat ggaaatatgc gacaaagtga
    43501 cggttacggg catgggtatg gtgatgcgtc ccatcactga accaggcgtc tattcctcag
    43561 gcattccgct gcaacccaac aaagtctggc gcaaaaccgc tgcactggtg atgaacattg
    43621 atgacatgag caagcgtctg aaatcgcttg agcgcaaggt taatcaacaa gactaacgtt
    43681 ccatcttttg ttcgccaaac tttacggcct gtctcattct tacgattgcg gcaggccgtg
    43741 ttattattgt cgtttcttat attttgacag gaagagtatc ttgactacta acactcatac
    43801 tctgcagatt gaagagattt tagaacttct gccgcaccgt ttccctttct tactggtgga
    43861 tcgcgtgctg gattttgaag aaggtcgttt tctgcgcgca gtaaaaaatg tctctgtcaa
    43921 tgagccattc ttccagggcc atttccctgg aaaaccgatt ttcccgggtg tgctgattct
    43981 ggaagcaatg gcacaggcaa caggtattct ggcgtttaaa agcgtaggaa aactggaacc
    44041 gggtgagctg tactatttcg ctggtattga cgaagcgcgc ttcaagcgcc cggtcgtgcc
    44101 tggcgatcaa atgatcatgg aagtcacttt cgaaaaaacg cgccgcggcc tgacccgttt
    44161 taaaggggtt gctctggtcg acggtaaagt agtttgcgaa gcaacgatga tgtgtgctcg
    44221 tagccgggag gcctgatacg tgattgataa atccgccttt gtgcatccaa ccgccattgt
    44281 ggaagagggc gcgtcgattg gcgcgaacgc acacattggt cctttttgta tcgttggacc
    44341 ccatgtcgaa attggtgagg gtaccgtact gaaatctcac gttgtcgtga atggccatac
    44401 taaaattggc cgcgataatg agatttatca gttcgcctcc atcggtgaag ttaaccagga
    44461 tctgaaatat gctggcgaac cgacccgtgt ggaaatcggc gatcgtaacc gcattcgcga
    44521 aagcgtcacc attcatcgtg gcacagtcca gggcggtgga ttgacgaagg tgggcagcga
    44581 caacttactg atgatcaacg cgcacattgc gcacgattgt acggtaggta accgctgtat
    44641 tctcgccaac aacgcaacgc tggcgggtca cgtatcggta gacgacttcg cgatcatcgg
    44701 cggcatgacc gcagtccatc agttctgcat cattggtgcg catgtgatgg ttggcggttg
    44761 ctccggtgtg gcgcaggacg tccctcctta tgtcattgcg cagggtaacc acgcaacgcc
    44821 gttcggtgtc aatatcgaag ggctgaagcg ccgcggattc agccgcgagg cgattaccgc
    44881 tatccgcaat gcgtataagc tgatttatcg tagcggtaaa acgctcgatg aagtgaaacc
    44941 ggaaattgct gaactggcgg aaacgtatcc ggaagtgaaa gcttttaccg atttctttgc
    45001 acgctcaacg cgcggtctga ttcgttaatg actgaacagc gtccattaac gattgccctg
    45061 gtcgccggag aaacctccgg cgatatcctg ggggccggtt taatccgcgc tctgaaagaa
    45121 cgtgtgccca acgcccgctt tgttggcgtt gccgggccac gaatgcaggc tgaaggctgc
    45181 gaagcctggt acgaaatgga agagttggcg gtgatgggca ttgttgaagt gctcggtcgt
    45241 ctgcgtcgct tactgcatat tcgtgccgat ctgacaaagc gttttggtga actaaagcca
    45301 gatgtttttg ttggcattga tgcgcctgac ttcaatatta ccctcgaagg taaccttaaa
    45361 aagcagggta tcaaaaccat tcattatgtc agtccgtccg tctgggcgtg gcgacagaaa
    45421 cgcgttttca aaataggcag agccaccgat ctggtgctcg catttctgcc tttcgaaaaa
    45481 gcgttttatg acaagtataa cgtgccgtgc cgttttatcg gtcacacgat ggctgatgcc
    45541 atgccgttag atccagataa aaacgccgcc cgtgatgtgc tgggtatccc tcacgatgcc
    45601 cactgtctgg cattgttgcc gggcagccgt ggtgcggaag tcgagatgct gagcgccgat
    45661 ttcctgaaaa cggcccagct tttgcgccag acgtaccccg atctggagat cgtggtgccg
    45721 ctggtgaatg ccaaacgccg cgagcagttt gaacgcatca aagctgaagt cgcgccagac
    45781 ctttcagttc atttgctgga tgggatgggc cgtgaggcga tggtcgccag cgatgcggca
    45841 ctactggcgt cggggacggc agccctggag tgtatgctgg cgaaatgccc gatggtggtg
    45901 ggatatcgca tgaagccttt taccttctgg ttggcgaagc ggctggtgaa aactgaatat
    45961 gtctcgctgc caaatctgct ggcgggcaga gagttagtca aagaattatt gcaggaagag
    46021 tgtgagccgc aaaaactggc tgcggcgctg ttaccgctgt tggcgaacgg gaaaaccagc
    46081 cacgcaatgc acgatacctt ccgtgaactg catcagcaga tccgctgcaa tgccgatgag
    46141 caggcggcac aagccgttct ggagttagca caatgatcga atttgtctat ccgcacacgc
    46201 agctggttgc gggtgtggat gaagtcggac gcgggccgtt agttggcgcg gtcgtcaccg
    46261 ctgcggtgat ccttgacccg gcgcgcccga ttgccgggct gaatgattcc aaaaagctga
    46321 gcgaaaaacg ccgtctggcg ctctatgaag agatcaaaga gaaagcgttg agctggagtc
    46381 tgggccgcgc ggaaccccac gaaatcgacg agctgaacat tcttcatgcg accatgctgg
    46441 cgatgcagcg tgccgtcgct gggctgcata ttgcgccgga atatgtgttg attgatggta
    46501 accgctgccc gaaattaccg atgcctgcga tggctgtggt gaaaggcgat agccgcgtac
    46561 cggaaatcag tgccgcgtct atcctggcga aagtgacgcg tgacgccgaa atggcggcgc
    46621 tggatattgt tttcccgcaa tatggttttg cccaacacaa agggtatcca accgcttttc
    46681 atctggaaaa actggctgaa tatggcgcga ctgaacatca tcgtcgcagc tttgggcctg
    46741 tcaaacgagc actgggactt gcgtcctgat tcttgtgtcg agattaagta aaccggaatc
    46801 tgaagatgtc tgaaccacgt ttcgtacacc tgcgggtgca cagcgactac tcgatgatcg
    46861 atggcctggc caaaaccgca ccgctggtaa aaaaggcggc ggcgttgggt atgccagcac
    46921 tggcgatcac cgatttcacc aacctttgcg gtctggtgaa gttctacgga gcgggacatg
    46981 gcgcagggat taagcctatc gtcggggcag attttaacgt ccagtgcgac ctgttgggtg
    47041 atgagttaac ccacctgacg gtactggcgg cgaacaatac cggctatcag aatctgacgt
    47101 tgctgatctc aaaagcgtat cagcgcgggt acggtgccgc cgggccgatc atcgatcgcg
    47161 actggcttat cgaattaaac gaagggttga tccttctttc cggcgggcgc atgggcgacg
    47221 tcggacgcag tcttttgcgt ggtaacagcg cgctggtaga tgagtgtgtc gcgttttatg
    47281 aagaacactt cccggatcgc tattttctcg agctgatccg caccggcagg ccggatgaag
    47341 aaagctatct gcacgcggcg gtggaactgg cggaagcgcg cggtttgccc gtcgtggcga
    47401 ctaacgatgt gcgctttatc gacagcagcg actttgacgc acacgaaatc cgcgtcgcga
    47461 tccacgacgg ctttaccctc gacgatccta aacgcccgcg taactattcg ccgcagcaat
    47521 atatgcgtag cgaagaggag atgtgcgagc tgtttgccga catccccgaa gctcttgcca
    47581 acaccgttga gatcgccaaa cgctgtaacg taactgtgcg tcttggtgaa tacttcctgc
    47641 cgcagttccc gaccggggac atgagcaccg aagattatct ggtcaagcgt gcaaaagagg
    47701 gcctggaaga gcgtctggcc tttttattcc ctgatgagga agaacgtgtt aagcgccgcc
    47761 cggaatatga cgaacgtctg gagactgaac ttcaggttat caaccagatg ggcttcccgg
    47821 gctacttcct tatcgttatg gaatttatcc agtggtcgaa agataacggt gtaccggtag
    47881 ggccaggccg tggctccggc gcaggctcac tggtggccta cgcgctgaaa atcaccgacc
    47941 tcgatccgct ggaatttgac ctgctgttcg aacgtttcct taacccggaa cgtgtctcca
    48001 tgcctgactt cgacgttgac ttctgtatgg agaaacgcga tcaggttatc gaacacgtag
    48061 cggacatgta cggtcgtgat gcggtatcgc agattatcac cttcggtacg atggcggcga
    48121 aagcggtgat ccgcgacgta ggccgcgtgc tggggcatcc gtacggcttt gtcgatcgta
    48181 tctcgaaact gatcccgccc gatccgggga tgacgctggc gaaagcgttt gaagccgagc
    48241 cgcagctgcc ggaaatctac gaagcggatg aagaagttaa ggcgctgatc gacatggcgc
    48301 gcaaactgga aggggtcacc cgtaacgccg gtaagcacgc cggtggggtg gttatcgcgc
    48361 cgaccaaaat taccgatttt gcgccgcttt attgcgatga agagggcaaa catccggtca
    48421 cccagtttga taaaagcgac gttgaatacg ccggactggt gaagttcgac ttccttggtt
    48481 tgcgtacgct caccatcatc aactgggcgc tggagatgat caacaagcgg cgggcgaaga
    48541 atggcgagcc gccgctggat atcgccgcga tcccgctgga tgacaagaaa agcttcgaca
    48601 tgctgcaacg ctcggaaacc acggcggtat tccagcttga atcgcgcggc atgaaggacc
    48661 tgatcaagcg tctgcaacct gactgcttcg aagatatgat cgcactggtg gcactgttcc
    48721 gccctggtcc gttgcagtca gggatggtgg ataactttat tgaccgtaaa catggtcgcg
    48781 aagaaatctc ctatccagac gtacagtggc agcatgaaag cctgaaaccg gtactggagc
    48841 caacctacgg cattatcctg tatcaggaac aggtcatgca gattgcgcag gtgctttctg
    48901 gttataccct cggtggcgcg gatatgctgc gtcgtgcgat gggtaagaaa aagccggaag
    48961 agatggcgaa gcagcgttct gtatttgctg aaggtgcaga aaagaacgga atcaacgccg
    49021 aactggcgat gaaaatcttc gacctggtgg agaaattcgc gggttacgga tttaacaaat
    49081 cgcactctgc ggcctatgct ttggtgtcat atcaaacatt atggctgaaa gcgcactatc
    49141 cggcagagtt tatggcggcg gtaatgaccg ccgatatgga caacaccgag aaggtggtgg
    49201 gcctggtgga tgagtgctgg cggatggggc tgaaaatcct gccaccagat ataaactccg
    49261 gtctttacca tttccacgtc aacgacgacg gcgaaatcgt gtatggtatt ggcgcgatca
    49321 aaggggtagg tgaaggtccg attgaggcga tcatcgaagc ccgtaataaa ggcggctact
    49381 tccgcgaact gtttgatctc tgcgcccgaa ccgacaccaa aaagttaaac cggcgggtgt
    49441 tggaaaaact gattatgtcc ggggcgtttg accgtcttgg gccacaccgc gcagcgctga
    49501 tgaactcgct gggcgatgcg ttaaaagcgg cagatcaaca cgcgaaagcg gaagccatcg
    49561 gtcaggccga tatgttcggc gtgctggccg aagagccgga acaaattgaa caatcctacg
    49621 ccagctgcca accgtggccg gagcaggtgg tattagatgg ggaacgtgaa acgttaggtc
    49681 tgtacctgac gggacaccct atcaaccagt atttgaaaga gattgagcgt tatgtcggag
    49741 gcgtaaggct gaaagacatg cacccgacag aacgtggtaa agtcatcacg gctgcggggc
    49801 tcgttgttgc cgcgcgggtt atggtcacca agcgcggcaa tcgtatcggt atctgcacgc
    49861 tggatgaccg ttccgggcgg ctggaagtga tgttgtttac tgacgccctg gataaatacc
    49921 agcaattgct ggaaaaagac cgcatactta tcgtcagcgg acaggtcagc tttgatgact
    49981 tcagcggtgg gcttaaaatg accgctcgcg aagtgatgga tattgacgaa gcccgggaaa
    50041 aatatgctcg cgggcttgct atctcgctga cggacaggca aattgatgac cagcttttaa
    50101 accgactccg tcagtctctg gaaccccacc gctctgggac aattccagta catctctact
    50161 atcagagggc ggatgcacgc gcgcggttgc gttttggcgc gacgtggcgt gtctctccga
    50221 gcgatcgttt attaaacgat ctccgtggcc tcattggttc ggagcaggtg gaactggagt
    50281 ttgactaata caggaatact atgagtctga atttccttga ttttgaacag ccgattgcag
    50341 agctggaagc gaaaatcgat tctctgactg cggttagccg tcaggatgag aaactggata
    50401 ttaacatcga tgaagaagtg catcgtctgc gtgaaaaaag tgtagaactg acgcgcaaaa
    50461 tcttcgccga tctcggtgca tggcagattg cgcaactggc acgccatcca cagcgtcctt
    50521 ataccctgga ttacgttcgc ctggcatttg atgaatttga cgaactggct ggcgaccgcg
    50581 cgtatgcaga cgataaagct atcgtcggtg gtatcgcccg tctcgatggt cgtccggtga
    50641 tgatcattgg tcatcaaaaa ggtcgtgaaa ccaaagaaaa aattcgccgt aactttggta
    50701 tgccagcacc agaaggttac cgcaaagctc tgcgcctgat gcaaatggct gaacgcttta
    50761 agatgccaat catcaccttt atcgacaccc cgggggctta tccgggcgtg ggcgcagaag
    50821 aacgcggtca gtctgaagct attgcacgca acctgcgtga aatgtctcgc ctcggtgtac
    50881 cggtagtttg tacggttatc ggtgaaggtg gttctggcgg cgcgctggcg attggcgtgg
    50941 gcgataaagt gaatatgctg caatacagca cctattccgt tatctcgccg gaaggttgtg
    51001 cgtccattct gtggaagagc gccgacaaag caccgctggc agctgaagcg atgggtatca
    51061 tcgctccgcg tctgaaagaa ctaaaactga tcgactccat catcccggaa ccgctgggtg
    51121 gtgctcaccg taacccggaa gcgatggcgg catcgttgaa agcgcaactg ctggcggatc
    51181 tggccgatct cgacgtgtta agcactgaag atttaaaaaa tcgtcgttat cagcgcctga
    51241 tgagctacgg ttacgcgtaa ttcgcaaaag ttctgaaaaa gggtcacttc ggtggccctt
    51301 ttttatcgcc acggtttgag caggctatga ttaaggaagg attttccagg aggaacacat
    51361 gaacatcatt gccattatgg gaccgcatgg cgtcttttat aaagatgagc ccattaaaga
    51421 actggagtcg gcgctggtgg cgcaaggctt tcagattatc tggccacaaa acagcgttga
    51481 tttgctgaaa tttatcgaac ataaccctcg aatttgcggc gtgatttttg actgggatga
    51541 gtacagtctc gatttatgta gcgatatcaa tcagcttaat gaatatctcc cgctttatgc
    51601 cttcatcaac acccactcga cgatggatgt cagcgtgcag gatatgcgga tggcgctctg
    51661 gttttttgaa tatgcgctgg ggcaggcgga agatatcgcc attcgtatgc gtcagtacac
    51721 cgacgaatat cttgataaca ttaccccacc gttcacgaaa gccttgttta cctacgtcaa
    51781 agagcggaag tacacctttt gtacgccggg tcatatgggc ggcaccgcat atcaaaaaag
    51841 cccggttggc tgtctgtttt atgacttttt cggcgggaat actcttaagg ctgatgtatc
    51901 tatttcggtc accgagcttg gttcgttgct cgaccacacc gggccacacc tggaagcgga
    51961 agagtacatc gcgcggactt tcggcgcgga acagagttat atcgttacca acggaacatc
    52021 gacgtcgaac aaaattgtgg gtatgtacgc cgcgccatcc ggcagtacgc tgttgatcga
    52081 ccgcaactgc cataaatcac tggcgcatct gttgatgatg aacgatgtag tgccagtctg
    52141 gctgaaaccg acgcgtaatg cgttggggat tctgggtggg atcccgcgcc gtgaatttac
    52201 tcgtgacagc atcgaagaga aagtcgctgc caccacgcag gcacaatggc cggttcatgc
    52261 ggtgattacc aactcaacct atgatggctt gctctacaac accgactgga tcaaacagac
    52321 gctggatgtc ccgtcgatcc acttcgattc cgcctgggtg ccgtacaccc attttcatcc
    52381 gatctaccag gggaaaagtg gtatgagcgg cgagcgtgtt gcgggaaaag tgatcttcga
    52441 aacgcaatcg acccacaaaa tgctggcggc gttatcgcag gcttcgctga tccacattaa
    52501 aggcgagtat gacgaagagg cctttaacga agcctttatg atgcatacca ccacctcgcc
    52561 cagttatccc attgttgctt cggttgagac ggcagcagcg atgctgcgtg gtaatccggg
    52621 taaacggctg attaaccgtt cggtggagcg ggcgctgcat tttcgcaaag aggtccagcg
    52681 gttgcgggaa gagtctgacg gttggttttt cgatatctgg caaccgccgc aggtggatga
    52741 agccgaatgc tggcccgttg cgcctggcga acagtggcac ggctttaacg atgcggatgc
    52801 cgatcatatg tttctcgatc cggttaaagt cactattttg acaccgggga tggacgaaca
    52861 gggcaatatg agtgaggagg ggatcccggc ggcgctggtg gcaaaattcc tcgacgaacg
    52921 tgggatcgta gtagagaaaa ccggccctta taacctgctg tttctcttta gtattggcat
    52981 cgataaaacc aaagcaatgg gattattgcg tgggttgacg gaatttaagc gctcttacga
    53041 tctcaacctg cggatcaaaa atatgctgcc cgatctctat gctgaagatc ccgatttcta
    53101 ccgcaatatg cgtattcagg atctggcgca agggatccat aagctgattc gtaaacacga
    53161 tcttcccggt ttgatgttac gggcattcga tactttgccg gagatgatca tgacgccaca
    53221 tcaggcatgg cagagacaga ttaaaggtga agtagaaacc attgcgctgg aacaactggt
    53281 cggtagagta tcggcaaata tgatcctgcc ttatccaccg ggcgtaccgc tgttgatgcc
    53341 tggagaaatg ctgaccaaag agagccgcac agtactcgat tttctactga tgctttgttc
    53401 cgtagggcaa cattaccccg gttttgaaac ggatattcac ggtgcgaaac aggacgaaga
    53461 cggcgtttac cgcgtacgag tcctaaaaat ggcgggataa cttgccagag cggcttccgg
    53521 gcgagtaacg tgctgttaac aaataaagga gacgttatgc tgggtttaaa acaggttcac
    53581 catattgcga ttattgcgac ggattatgcg gtgagcaaag ctttctactg cgatattctt
    53641 ggtttcacgc tgcaaagcga agtctatcgc gaagcgcgcg actcgtggaa aggggatttg
    53701 gcgcttaatg ggcaatatgt gattgagctt ttctcatttc cgttcccgcc ggagcgcccc
    53761 agccgaccgg aagcttgcgg cctgcgtcat ctggctttta gcgttgatga catcgatgcg
    53821 gcagtggcgc accttgaaag ccataacgtg aagtgtgaag ccatccgtgt cgatccatac
    53881 acgcaaaaac gcttcacctt ctttaacgat ccggacgggc tgccgttgga actgtatgag
    53941 cagtaaggct tgtcatcgcg gcatttgccc ggtaacgtgc cgggcattgc tactgtaaaa
    54001 tcgcaccatc atgacactca cgctcaatag acaacttctc acctcacgcc agattctggt
    54061 ggcctttagc ggcgggcttg actccaccgt tctgctgcat cagttggtgc agtggcggac
    54121 ggaaaatccg ggcgtcactc tacgcgctat ccatgtgcat cacggtttaa gtgccaatgc
    54181 cgatgcctgg gttaggcatt gcgagaacat ctgccaacag tggcaggtgc cgctggtggt
    54241 cgagcgcgtg caacttgcgc aagaaggact gggcattgag gcccaggcgc ggcaggcgcg
    54301 ttatcaggca tttgcccgca ccttgttgcc cggtgaagtg ctggtcaccg cgcaacatct
    54361 cgacgatcaa tgtgaaacct ttctgctggc gctaaaacgt ggtagcggcc ctgccgggct
    54421 ttcggctatg gcggaagttt cggagtttgc cggaacacgg cttatccgcc cgttgctcgc
    54481 ccgcacgcgg ggggaactgg cgcagtgggc attagcgcat ggtttacgct ggattgaaga
    54541 cgaaagtaat caggacgact catacgatcg taactttctg cgcctgcgtg tagtgccgtt
    54601 attgcagcag cgttggccgc attttgccga agcaacggct cgcagcgccg cactttgtgc
    54661 tgaacaagag agcctgctgg atgaattgct ggcagatgat ttagcgcact gccaaacgcc
    54721 gcaggggacg ctgcagattg cgccaatgct ggcgatgagt gatgcccgtc gcgcggcaat
    54781 tatccgccgc tggctggcag ggcagaatgc accgatgcct tcccgcgacg cgctggtgcg
    54841 gatctggcag gaagtggcgc tggcgcggga agatgcctcc ccctgtttac gctttggcgc
    54901 gtttgaaata cgccgctatc aatcgcaact gtggtggatt aaatccgttg ccgggcaaag
    54961 cgaaaccatt gtgccgtggc agacgtggct acaaccgctg gaattaccgg cggggctggg
    55021 aagtgtacag cttactgcgg gaggcgatat tcgccctccg cgtgcagacg aagcggtcag
    55081 cgtgcgtttc aaagcgccag gattgctgca tattgtcggt cgcaacggcg gacgtaagct
    55141 gaagaaaatc tggcaggagc tgggcgtgcc gccgtggcta cgtgacacca cgccactgct
    55201 gttttatggc gaaacgctga ttgcggcggc aggggtattt gtgacgcaag aaggtgtggc
    55261 tgaaggtgag aagggcgtaa gttttgtctg gcagaaaacg cttagttaag tgaaagccgg
    55321 ataagacgca tcaaacgtcg catccggcaa aagtcaatca ggattcgctt accaccaccg
    55381 taccgatttc cgggtggcta aagctggtaa ttttatccag acgtagctca cgggtttcgc
    55441 cagcggcctc gacgaccaga tactccacat ttttgcggga gactaaatcg ctggcttttg
    55501 cctgcaattt ttcgccatct ttcagctcaa gtgtcagcat taaatgatgc tggcaggcga
    55561 gctcaagatt atcgtaatca tcacaattga ttggttgata cgtatcattc attgacataa
    55621 tcgctcacca gtaagtttgc cgcagcgtat gctgcttttt ccctgacagc ctcagaaagg
    55681 gcgtcgtcgg cagccatttc attcagcact ttcaaaacgc agcccagcgc gtccggaacg
    55741 tatcctaagt ctccgctggc gatttccgcg taccgcttgc gtattaactc acaatatttt
    55801 tccacatgcc ctcctgtcag cactctgact taaccgtgga tgcaagtcta agcctacgaa
    55861 gataaactct gtttcgcaag gtgactatac cacactcatt tctgcaatat cagcgccgca
    55921 actgcacgta ttccgttaca atggcctcct gattcgaaag gagttttctt atggcgctta
    55981 aagcgacaat ttataaagcg acggttaatg tggccgatct cgaccgcaac cagtttctcg
    56041 atgcctctct gacgctggcg cgccaccctt cagaaaccca ggagcgtatg atgctgcgct
    56101 tgctggcgtg gttgaaatat gctgatgaac gtctgcaatt tacccgtggt ttgtgtgccg
    56161 atgatgagcc ggaagcgtgg ctgcgtaacg atcacctggg cattgatttg tggattgagc
    56221 tggggctgcc ggatgagcgg cggattaaga aagcctgcac ccaggccgca gaagtggcgc
    56281 tgtttaccta taatagtcgg gcggcgcaaa tctggtggca gcaaaatcag agcaaatgtg
    56341 tgcagtttgc caatctttcc gtctggtatc tggacgatga acaactggcg aaagtaagcg
    56401 cctttgccga tcgtaccatg acgctgcagg caacgattca ggatggcgtg atctggttat
    56461 cggatgataa gaataatctg gaagtgaact taaccgcctg gcaacaacct tcatgattgt
    56521 gatttcccga catgttgcta ttcccgatgg tgagcttgag atcaccgcca ttcgtgcgca
    56581 gggcgcgggc gggcagcatg ttaataagac ctcaacggct attcatctgc gttttgacat
    56641 tcgggcgtcc agcctgccag agtattacaa agagcgtctg ctcgccgcca gccatcattt
    56701 gatcagcagt gatggcgtga ttgtcattaa ggcacaggaa taccgcagtc aggaactgaa
    56761 ccgcgaagca gctctggccc ggctggtggc tgtgattaaa gaattaacaa cagaacaaaa
    56821 agcccgacga cccacgcggc ccacccgtgc atcgaaagag cgcaggctgg catcgaaagc
    56881 acaaaaatca agcgtgaagg cgatgcgcgg caaagtgcgc agcggtcggg aataaaaaga
    56941 aggaatggat ggtgaaaaaa gcgatagtga cagcgatggc tgtaatcagc ctctttactc
    57001 tgatgggttg taataatcgg gccgaagtcg atacgctttc tccggcgcag gttgccgaac
    57061 tgaaaccgat gccgcaaagt tggcgcggcg tgctgccgtg tgccgattgc gaaggaatcg
    57121 aaacctctct gttcctcgaa aaagacggaa catgggtgat gaatgagcgt tatctcggtg
    57181 ctcgtgaaga accttcctcc ttcgcttcct acggtacatg ggcgcgaacc gctgacaagc
    57241 tggtattaac cgacagcaaa ggtgaaaagt catattatcg ggcgaaaggc gatgcgctgg
    57301 agatgctcga tcgtgaaggc aatccgattg aatcgcagtt caactatacg ctggaaccgg
    57361 cacaatccag tttacccatg acgccgatga ccctgcgggg catgtatttt tatatggctg
    57421 atgcggcgac cttcactgat tgcgcgaccg gaaaacgttt catggtagcg aataacgcag
    57481 agctggagcg tggctacctg gctgcgcgcg gccacagtga aaaaccggtg ttactgtcag
    57541 tagaaggtca ctttacgctt gaggctaatc cggataccgg tgcgccaacc aaagtattag
    57601 cacccgatac ggcagggaaa ttttacccaa accaggattg cagtagtttg gggcagtaac
    57661 ccgtcttgag acagaaacaa acgcaaaaca ggccagaagg atatatttca acattttgaa
    57721 tttgcacgtt ttttgtaggc cggataaggc gtttacgccg catccggcaa tggtgctcaa
    57781 cgcctgatgc gacgctggcg cgtcttatca tgcctacaac ccccctcata ccttaaggcg
    57841 gataaggcat ttactttatc accgggtcaa accaacaaag cgacccgcct tgatataaat
    57901 ccctggcttc aggtgcccaa catactgtaa ctgcgtttct ggcttaaacg cagagacatc
    57961 accaatacgc atatgcatca gatcggcagg gctaatccag cccgattgcg ccagcgtaag
    58021 cggatgcccg gctttggcaa atgcatcagt gacaaattcc gaacaaaacc acgacttttt
    58081 gtctccttcg cccacactgc ttaactgcgc tttcgccagg ccgctgacgc actgttggcg
    58141 aaaatctgcg gaaaaaggat tcagtgagca tatctggcgg gtcaccataa atggaatgaa
    58201 ctcgacaatg ccgcgatagt tatacccgct atctttaatt ttgttggcga acgcggtgat
    58261 ttctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcgctatg
    58321 cttcatcgcc tttttaaggg aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt
    58381 attttcaccc agatagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
    58441 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtac
    58501 cgttatttct tttattgctt gttcggtgaa agagctttga tgctggaatt tgacagccca
    58561 tgtttttgcc tcgacatcta ccgctgtggc agaggagtct ggctggctga tatcaacggt
    58621 acaggccgaa agtaaaagaa agcaggggag aagcaggcgg cagtacgcct ttggtttatc
    58681 cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc tggcatcagc
    58741 ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac tgtttctcgc
    58801 cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa
    58861 tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga cgctctttgc
    58921 ggtcatccag cagcacttcg ataccttgcg cgcgcagttc gctgtacagt ttctcagcaa
    58981 gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc tggaacggcg
    59041 cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc gccgcagcta
    59101 ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta cggccatctt
    59161 caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg aagatgtgac
    59221 caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt ggatcgccag
    59281 ccaccacgtt acggatatcc gcaacttccg gggtagcgac atcgcgatcc cagttgatgc
    59341 cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc gccgcaacgg
    59401 tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc ggaccggcgt
    59461 taaccacggc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca acctgcggca
    59521 gtttttctgc tttaacttcg ttcagttcgt ggtcaccgcg caccagcagt gcaaccagcg
    59581 ggaagctact gccttcaacc gctttaacca gcagagtctt aaccgttttc tcaatcggca
    59641 gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca
    59701 tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc agttcaatgt
    59761 tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca
    59821 cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcctgt acggcgcgga
    59881 aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca tcgtaggttt
    59941 cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg aattcgcggg
    60001 aacgcatgac gccgaaacgc ggacgcactt catcgcggaa cttggtctgg atctgataga
    60061 agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca gtgataactt
    60121 cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt
    60181 ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc tgaaccaccg
    60241 gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc acgcacgatg ttttcgactt
    60301 ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc agcttgcgga
    60361 tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca ggtgtctcct
    60421 tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag ttggaaggta
    60481 gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa
    60541 atgtcaaaag agaagggcgt gaatttaacg cggttccagc gcaaagactt caaaacctgc
    60601 gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag acggcatagg ttttgcccgt
    60661 ttcctcacct ttacgatagg ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag
    60721 cgttaactgc ggataacgct tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa
    60781 actcaccgtc atctctgccg ctggcgcact ttgcgcatag ctggcactgg catcgggaag
    60841 cgattcggca aaggggagat acggtttgat atccaccacc ggcgtaccat cgaccagatc
    60901 cagactgccg agcttcagaa tcacgctgtc tttatggcaa acaacctctt tcagctctac
    60961 cagcgacata ccaattgggt tagggcgaaa agtggaacgc gtagcgaaaa cacccattct
    61021 ggcattaccg ccaagacgag gcggacgcac agtcggacgc cagccgcctt ccatcgtttg
    61081 atgaaagacg aaaagtatcc ataaatggct gaatgcttcc aggccgcgaa cggcgtcggc
    61141 ctggttgtag ggagcaatga gatgcagttc gccgttggcg ctttttacca gacctggctg
    61201 gcgcggaacg gcgaattttt ctttataggg cgagcgaata acgcctattt gctcgaactg
    61261 gaaactgctc atttcgccgt aatgttaagc gcagaaccga tacatacagc ctgacgatag
    61321 cagcctggcg taccgctggt gacttcgcag ctatgcagta ataccgcatt ggctttcatt
    61381 ttcgaggcgt tgatttgcat ccgcttacgc gcggttggaa tgctcggcgg agagtcctga
    61441 ttagaggcct ggcaagagtc gccactgact tcaccgagat cgcggaacgg tttgccgact
    61501 aattcttctg cattggtata aattcggacc ggcgtggcgc gcggcgcttt cggttttgca
    61561 ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt cgacagggga tctgcttaac
    61621 atggaacagc cgcttagcat gagtgctact aaacagatcg gtaaagcacg catagtattt
    61681 cctcaatgta tgatcaaaac gtcaatattg aatcaggagc ttgtaaaaat gacaagacgg
    61741 gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac agcctaaaaa ttaccagcct
    61801 ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt aaacttcgtc agactgataa
    61861 gcctggacga atttcttcac gttctcggcg tctttgttat cttcacgcgt tacgatcagg
    61921 tttacgtacg gggagtcttt atcttcgaca aagataccgt ctttcgctgg agtcaggcca
    61981 atctggctgg cataggtggt attgataact gccagagcga tttgcgcatc gtccagagaa
    62041 cgcggcagtt gcggtgcttc cagttcaaca attttcagat tttttgggtt ctcaacaaca
    62101 tcaagaacgg tcggcagcag gccaacgcca tctttcagtt tgatcaagcc cactttttgc
    62161 agcagcagca gtgaacgacc aaggttagtt gggtcgtttg gcacggcaac ctgcgaacca
    62221 tcctgcagtt catccagtga tttgattttc ttggagtaac cagcaatcgg ataaacaaaa
    62281 gtgttgccta ctgcgaccag tttgtagcca cgatctttca gttgctgatc aaggtacggt
    62341 ttatgctgga aggcgttggc gtcgatatcg cctttgctca atgcttcgtt tggcagaaca
    62401 tagtcgttga aggttaccag ctcaacgtcc aggccatatt tgtctttcgc aactttctgc
    62461 gcgacttctg caacctgctg ttcggcacca acaatcacgc cgactttaat gtggtttgga
    62521 tctttttcat cctgaccgca gcctaccagt gccagtgatc caatcagggc tcccactgcc
    62581 gcaaaggttt tgaatttgaa cgccatacct tattccttct tcaattattt atgttgtgtt
    62641 gaacgttact tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg aattaaataa
    62701 accagaatga ccagcaatac cagtaccgta ttcatcaccg tcgcgttgta gccgatgtag
    62761 ccatactgat agccaatctg acctaaacca ccggcaccga ctgcaccacc catcgcggaa
    62821 taaccgacca gggtaatcag ggtgatagtt gccgcattca ccagacccgg caacgcttcc
    62881 ggtaacagca ctttacggac gatctgcatt ggcgtggcac ccattgcgcg ggaagcttca
    62941 attaacccgg ttgggatctc cagcagagcg ttctcgacca tacgggcaat aaacggtgct
    63001 gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa tcgatgtacc gacaataacg
    63061 cgggtaaacg gaatcatcca taccagcaag ataatgaacg ggatggaacg gaaaatgttc
    63121 acaatcgcag aaacggtacg atacagcttc gcgttagcaa taatttgccc cggacgcgtg
    63181 acataaagca ggacgccaac cggcagacca atcacaaacc caaaaaatcc ggatacgaag
    63241 gtcattgcca gcgtttccca tacgccacga accagcagcc acatcatcgg ctcagacata
    63301 acccagtacc tctactttta catggtgttc ctgcagccag gcaatggcgg cttgcgtatc
    63361 ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat ttaacgccac cagcgtagtc
    63421 catctgcgcg ctaataatgt tgttgttgac gttgaaacga cgcgcggttt cagaaagcag
    63481 tggggcatcg accgattgac cggtaaactc cagacgcagc atcggcacgc agtcagtgaa
    63541 tggctccgct tgcagacgtt cctggtaatc ttccgggata tccagatgca gggttgactg
    63601 aataaacttc tgcgccagcg gcgttttcgg atgtgagaac acttcactta ccgtgtcctg
    63661 ctcgattagt tcgccattgc tgatgaccgc aacgcaatca caaatacgct tcacaacgtc
    63721 catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg cggttgatgt ctttcagcag
    63781 ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat cacacagcaa
    63841 tactttggga ttgctggcta atgcacgggc aatcgccaca cgttgtttct gcccaccgga
    63901 aagattcgac gggtagctat catgcttatc gccaagacca accaatgaca gcaattccgt
    63961 cacgcgacgt ttgatctcgt ctttcggtgt attgtccagc tccagcggca gagccacgtt
    64021 gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac caatctggcg
    64081 acgagctttg gtcaactcgg attctgacag cgtggtcagt tcctggccat cgaccagcac
    64141 gctaccctcg gttgggcgct ccagcaggtt tacacaacgt ataagcgtac tcttacccgc
    64201 gcctgaggca ccgataacgc cataaatttg tccagctggc acatgcaggc tgacgttgtt
    64261 caacgcctgg atgatgcggg tgccctggtg gaacactttg gtgatattcg aaagttttat
    64321 cattgattat ttattatcgt cattaagtta gtcgtggcat ctcgaatgcc tgaaacgggc
    64381 aacgccgtca atgaaatgga tgttaaggca tccagacgtc taaatcaatc aggtttatgc
    64441 gaagagcact ttcttgcagg tcgaaacatg cgatactagc gtcacatgcc ttattaagga
    64501 gctataaaag gtggcgaaga gcgtacccgc aatttttctt gaccgtgatg gcaccattaa
    64561 tgtcgatcac ggctatgtcc atgagatcga caactttgaa tttatcaacg gtgttattga
    64621 cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg gtagtaacca accagtctgg
    64681 cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg ctgaccgagt ggatggactg
    64741 gtcgctggcg gaccgagatg tcgatctgga tggtatctat tattgcccgc atcatccgca
    64801 gggtagtgtt gaagagtttc gccaggtctg cgattgccgc aaaccacatc cggggatgct
    64861 tttgtcagca cgcgattatt tgcatattga tatggccgct tcttatatgg tgggcgataa
    64921 attagaagat atgcaggcag cggttgcggc gaacgtggga acaaaagtgc tggtgcgtac
    64981 gggtaaacct attacacctg aagcagaaaa cgcggcagat tgggtgttaa atagcctggc
    65041 agacctgccg caagcgataa aaaagcagca aaaaccggcg caatgattaa aagatgagcg
    65101 gttgaaataa aaatgcattt ttccgcttgt cttcctgagc cgactcccta taatgcgcct
    65161 ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa
    65221 atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca
    65281 gtg
//
LOCUS       DAZGBC010000009        60678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGBC010000009 DAZGBC010000000
VERSION     DAZGBC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.009"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..1480)
                     /gene="rhlE"
                     /locus_tag="ACHK1J_000577"
     CDS             complement(116..1480)
                     /gene="rhlE"
                     /locus_tag="ACHK1J_000577"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO7909915.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQLRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1709..2380
                     /gene="cecR"
                     /locus_tag="ACHK1J_000578"
     CDS             1709..2380
                     /gene="cecR"
                     /locus_tag="ACHK1J_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO7909916.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2383..3378
                     /gene="hlyD"
                     /locus_tag="ACHK1J_000579"
     CDS             2383..3378
                     /gene="hlyD"
                     /locus_tag="ACHK1J_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO7909917.1"
                     /translation="MKKPVVIGLAVVALAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3371..5107
                     /gene="ybhF"
                     /locus_tag="ACHK1J_000580"
     CDS             3371..5107
                     /gene="ybhF"
                     /locus_tag="ACHK1J_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7909918.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5100..6233
                     /gene="ybhS"
                     /locus_tag="ACHK1J_000581"
     CDS             5100..6233
                     /gene="ybhS"
                     /locus_tag="ACHK1J_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7909919.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6244..7350
                     /gene="ybhR"
                     /locus_tag="ACHK1J_000582"
     CDS             6244..7350
                     /gene="ybhR"
                     /locus_tag="ACHK1J_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7909920.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTYQTVPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7312..7722)
                     /gene="ybhQ"
                     /locus_tag="ACHK1J_000583"
     CDS             complement(7312..7722)
                     /gene="ybhQ"
                     /locus_tag="ACHK1J_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO7909921.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7855..8616
                     /gene="ybhP"
                     /locus_tag="ACHK1J_000584"
     CDS             7855..8616
                     /gene="ybhP"
                     /locus_tag="ACHK1J_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7909922.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8613..9854
                     /gene="clsB"
                     /locus_tag="ACHK1J_000585"
     CDS             8613..9854
                     /gene="clsB"
                     /locus_tag="ACHK1J_000585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO7909923.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9854..10810
                     /gene="ybhN"
                     /locus_tag="ACHK1J_000586"
     CDS             9854..10810
                     /gene="ybhN"
                     /locus_tag="ACHK1J_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO7909924.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGENTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10846..11559)
                     /gene="ybhM"
                     /locus_tag="ACHK1J_000587"
     CDS             complement(10846..11559)
                     /gene="ybhM"
                     /locus_tag="ACHK1J_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO7909925.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11765..12469)
                     /gene="ybhL"
                     /locus_tag="ACHK1J_000588"
     CDS             complement(11765..12469)
                     /gene="ybhL"
                     /locus_tag="ACHK1J_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO7909926.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12605..13057)
                     /gene="moaE"
                     /locus_tag="ACHK1J_000589"
     CDS             complement(12605..13057)
                     /gene="moaE"
                     /locus_tag="ACHK1J_000589"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO7909927.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13059..13304)
                     /gene="moaD"
                     /locus_tag="ACHK1J_000590"
     CDS             complement(13059..13304)
                     /gene="moaD"
                     /locus_tag="ACHK1J_000590"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO7909928.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13297..13782)
                     /gene="moaC"
                     /locus_tag="ACHK1J_000591"
     CDS             complement(13297..13782)
                     /gene="moaC"
                     /locus_tag="ACHK1J_000591"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO7909929.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13785..14297)
                     /gene="moaB"
                     /locus_tag="ACHK1J_000592"
     CDS             complement(13785..14297)
                     /gene="moaB"
                     /locus_tag="ACHK1J_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO7909930.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14319..15308)
                     /gene="moaA"
                     /locus_tag="ACHK1J_000593"
     CDS             complement(14319..15308)
                     /gene="moaA"
                     /locus_tag="ACHK1J_000593"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO7909931.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15296..15440)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15705..16613
                     /gene="yvcK"
                     /locus_tag="ACHK1J_000594"
     CDS             15705..16613
                     /gene="yvcK"
                     /locus_tag="ACHK1J_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO7909932.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16804..18825)
                     /gene="uvrB"
                     /locus_tag="ACHK1J_000595"
     CDS             complement(16804..18825)
                     /gene="uvrB"
                     /locus_tag="ACHK1J_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO7909933.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19404..20081)
                     /gene="bioD"
                     /locus_tag="ACHK1J_000596"
     CDS             complement(19404..20081)
                     /gene="bioD"
                     /locus_tag="ACHK1J_000596"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7909934.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPRLAENPENAATGK
                     YINLALL"
     gene            complement(20074..20829)
                     /gene="bioC"
                     /locus_tag="ACHK1J_000597"
     CDS             complement(20074..20829)
                     /gene="bioC"
                     /locus_tag="ACHK1J_000597"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO7909935.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLVWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYRHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20816..21970)
                     /gene="bioF"
                     /locus_tag="ACHK1J_000598"
     CDS             complement(20816..21970)
                     /gene="bioF"
                     /locus_tag="ACHK1J_000598"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO7909936.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVTQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFIHNDVAHLAR
                     LLASPCSGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTSAHEMQDIDRLLEVLHGNG"
     gene            complement(21967..23007)
                     /gene="bioB"
                     /locus_tag="ACHK1J_000599"
     CDS             complement(21967..23007)
                     /gene="bioB"
                     /locus_tag="ACHK1J_000599"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO7909937.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23094..24383
                     /gene="bioA"
                     /locus_tag="ACHK1J_000600"
     CDS             23094..24383
                     /gene="bioA"
                     /locus_tag="ACHK1J_000600"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO7909938.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIIHPQQLQRLTAGVNRAVQDETFFCQ"
     gene            24442..24918
                     /gene="ybhB"
                     /locus_tag="ACHK1J_000601"
     CDS             24442..24918
                     /gene="ybhB"
                     /locus_tag="ACHK1J_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO7909939.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25070..26353
                     /gene="ybhC"
                     /locus_tag="ACHK1J_000602"
     CDS             25070..26353
                     /gene="ybhC"
                     /locus_tag="ACHK1J_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO7909940.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26494..28755)
                     /gene="ybhJ"
                     /locus_tag="ACHK1J_000603"
     CDS             complement(26494..28755)
                     /gene="ybhJ"
                     /locus_tag="ACHK1J_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO7909941.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(28938..30371)
                     /gene="ybhI"
                     /locus_tag="ACHK1J_000604"
     CDS             complement(28938..30371)
                     /gene="ybhI"
                     /locus_tag="ACHK1J_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO7909942.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTIGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(30447..31499)
                     /gene="ybhH"
                     /locus_tag="ACHK1J_000605"
     CDS             complement(30447..31499)
                     /gene="ybhH"
                     /locus_tag="ACHK1J_000605"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO7909943.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIIPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            31683..32636
                     /gene="ybhD"
                     /locus_tag="ACHK1J_000606"
     CDS             31683..32636
                     /gene="ybhD"
                     /locus_tag="ACHK1J_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7909944.1"
                     /translation="MKHELSSMKAFVILAESNSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(32677..33672)
                     /gene="pgl"
                     /locus_tag="ACHK1J_000607"
     CDS             complement(32677..33672)
                     /gene="pgl"
                     /locus_tag="ACHK1J_000607"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO7909945.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            33827..34645
                     /gene="ybhA"
                     /locus_tag="ACHK1J_000608"
     CDS             33827..34645
                     /gene="ybhA"
                     /locus_tag="ACHK1J_000608"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO7909946.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(34646..35704)
                     /gene="modC"
                     /locus_tag="ACHK1J_000609"
     CDS             complement(34646..35704)
                     /gene="modC"
                     /locus_tag="ACHK1J_000609"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO7909947.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(35707..36396)
                     /gene="modB"
                     /locus_tag="ACHK1J_000610"
     CDS             complement(35707..36396)
                     /gene="modB"
                     /locus_tag="ACHK1J_000610"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO7909948.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(36396..37169)
                     /gene="modA"
                     /locus_tag="ACHK1J_000611"
     CDS             complement(36396..37169)
                     /gene="modA"
                     /locus_tag="ACHK1J_000611"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7909949.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(37336..37485)
                     /gene="acrZ"
                     /locus_tag="ACHK1J_000612"
     CDS             complement(37336..37485)
                     /gene="acrZ"
                     /locus_tag="ACHK1J_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO7909950.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            37614..38402
                     /gene="modE"
                     /locus_tag="ACHK1J_000613"
     CDS             37614..38402
                     /gene="modE"
                     /locus_tag="ACHK1J_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO7909951.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            38470..39942
                     /gene="modF"
                     /locus_tag="ACHK1J_000614"
     CDS             38470..39942
                     /gene="modF"
                     /locus_tag="ACHK1J_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO7909952.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILCGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            40204..41220
                     /gene="galE"
                     /locus_tag="ACHK1J_000615"
     CDS             40204..41220
                     /gene="galE"
                     /locus_tag="ACHK1J_000615"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO7909953.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPMFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMCAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            41230..42276
                     /gene="galT"
                     /locus_tag="ACHK1J_000616"
     CDS             41230..42276
                     /gene="galT"
                     /locus_tag="ACHK1J_000616"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO7909954.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            42280..43428
                     /gene="galK"
                     /locus_tag="ACHK1J_000617"
     CDS             42280..43428
                     /gene="galK"
                     /locus_tag="ACHK1J_000617"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO7909955.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            43422..44462
                     /gene="galM"
                     /locus_tag="ACHK1J_000618"
     CDS             43422..44462
                     /gene="galM"
                     /locus_tag="ACHK1J_000618"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO7909956.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALTSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHLWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            44664..45416
                     /gene="gpmA"
                     /locus_tag="ACHK1J_000619"
     CDS             44664..45416
                     /gene="gpmA"
                     /locus_tag="ACHK1J_000619"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO7909957.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(45574..46626)
                     /gene="aroG"
                     /locus_tag="ACHK1J_000620"
     CDS             complement(45574..46626)
                     /gene="aroG"
                     /locus_tag="ACHK1J_000620"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO7909958.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            46942..47322
                     /gene="ybgS"
                     /locus_tag="ACHK1J_000621"
     CDS             46942..47322
                     /gene="ybgS"
                     /locus_tag="ACHK1J_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO7909959.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            47436..48377
                     /gene="zitB"
                     /locus_tag="ACHK1J_000622"
     CDS             47436..48377
                     /gene="zitB"
                     /locus_tag="ACHK1J_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO7909960.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(48374..49093)
                     /gene="pnuC"
                     /locus_tag="ACHK1J_000623"
     CDS             complement(48374..49093)
                     /gene="pnuC"
                     /locus_tag="ACHK1J_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO7909961.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALTWLAVCVIAIGLMTVFINPVFAFLTRVAVIIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(49131..50174)
                     /gene="nadA"
                     /locus_tag="ACHK1J_000624"
     CDS             complement(49131..50174)
                     /gene="nadA"
                     /locus_tag="ACHK1J_000624"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO7909962.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(50452..50577)
                     /locus_tag="ACHK1J_000625"
     ncRNA           complement(50452..50577)
                     /ncRNA_class="other"
                     /locus_tag="ACHK1J_000625"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(50608..50683)
                     /locus_tag="ACHK1J_000626"
     tRNA            complement(50608..50683)
                     /locus_tag="ACHK1J_000626"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50648..50650),aa:Lys,seq:ttt)
     gene            complement(50717..50792)
                     /locus_tag="ACHK1J_000627"
     tRNA            complement(50717..50792)
                     /locus_tag="ACHK1J_000627"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50757..50759),aa:Lys,seq:ttt)
     gene            complement(50939..51014)
                     /locus_tag="ACHK1J_000628"
     tRNA            complement(50939..51014)
                     /locus_tag="ACHK1J_000628"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50979..50981),aa:Lys,seq:ttt)
     gene            complement(51018..51093)
                     /locus_tag="ACHK1J_000629"
     tRNA            complement(51018..51093)
                     /locus_tag="ACHK1J_000629"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51058..51060),aa:Val,seq:tac)
     gene            complement(51145..51220)
                     /locus_tag="ACHK1J_000630"
     tRNA            complement(51145..51220)
                     /locus_tag="ACHK1J_000630"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51185..51187),aa:Lys,seq:ttt)
     gene            complement(51223..51298)
                     /locus_tag="ACHK1J_000631"
     tRNA            complement(51223..51298)
                     /locus_tag="ACHK1J_000631"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51263..51265),aa:Val,seq:tac)
     gene            complement(51335..51410)
                     /locus_tag="ACHK1J_000632"
     tRNA            complement(51335..51410)
                     /locus_tag="ACHK1J_000632"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51375..51377),aa:Lys,seq:ttt)
     gene            complement(51575..52366)
                     /gene="cpoB"
                     /locus_tag="ACHK1J_000633"
     CDS             complement(51575..52366)
                     /gene="cpoB"
                     /locus_tag="ACHK1J_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO7909963.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(52376..52897)
                     /gene="pal"
                     /locus_tag="ACHK1J_000634"
     CDS             complement(52376..52897)
                     /gene="pal"
                     /locus_tag="ACHK1J_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO7909964.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(52932..54224)
                     /gene="tolB"
                     /locus_tag="ACHK1J_000635"
     CDS             complement(52932..54224)
                     /gene="tolB"
                     /locus_tag="ACHK1J_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO7909965.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(54357..55622)
                     /gene="tolA"
                     /locus_tag="ACHK1J_000636"
     CDS             complement(54357..55622)
                     /gene="tolA"
                     /locus_tag="ACHK1J_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO7909966.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(55687..56115)
                     /gene="tolR"
                     /locus_tag="ACHK1J_000637"
     CDS             complement(55687..56115)
                     /gene="tolR"
                     /locus_tag="ACHK1J_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO7909967.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(56119..56811)
                     /gene="tolQ"
                     /locus_tag="ACHK1J_000638"
     CDS             complement(56119..56811)
                     /gene="tolQ"
                     /locus_tag="ACHK1J_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO7909968.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(56808..57212)
                     /gene="ybgC"
                     /locus_tag="ACHK1J_000639"
     CDS             complement(56808..57212)
                     /gene="ybgC"
                     /locus_tag="ACHK1J_000639"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7909969.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(57362..57655)
                     /gene="ybgE"
                     /locus_tag="ACHK1J_000640"
     CDS             complement(57362..57655)
                     /gene="ybgE"
                     /locus_tag="ACHK1J_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO7909970.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     DLEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(57655..57768)
                     /gene="cydX"
                     /locus_tag="ACHK1J_000641"
     CDS             complement(57655..57768)
                     /gene="cydX"
                     /locus_tag="ACHK1J_000641"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO7909971.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(57783..58922)
                     /gene="cydB"
                     /locus_tag="ACHK1J_000642"
     CDS             complement(57783..58922)
                     /gene="cydB"
                     /locus_tag="ACHK1J_000642"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7909972.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(58938..60506)
                     /gene="cydA"
                     /locus_tag="ACHK1J_000643"
     CDS             complement(58938..60506)
                     /gene="cydA"
                     /locus_tag="ACHK1J_000643"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO7909973.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        14728 a        16159 c        15320 g        14471 t
ORIGIN      
        1 atagatcata aagcaaattt attaaaacaa taaaaacaga catgtcatgc atgtctgacc
       61 gtgttacctg aataaagggc aaaagcctgt gatactcttt tgcccttaca tggtattact
      121 gcgcagcggc aggtttacgc ggacggcggc gacgttgttg ttcgcctgcc ggtttggcat
      181 cgccgaggcg gcgagacggt ttttctgcag gcttcgcgct tgcagatttt gcaccacctt
      241 ctccacggcg tagttgctgt tgaccgcgac caccgccctg cccacgacca ccgccgccac
      301 gttgctggcg accgttctgg atcggttcgg ctttgattga cgggtccggc tcatagcccg
      361 gaatcgcaat gcgcgggatc tcttttttca gcagtttttc gatatcacgc agcagtttgt
      421 gttcatcaac acacaccaac gacaacgctt ctccggtggc tgcggcacga ccggtacgcc
      481 cgatacggtg gacataatct tccggtacgt ttggcagttc atagttgacg acgtgcggca
      541 actcttcgat atccagaccg cgcgcagcga tgtcagttgc caccagtaca cgaatatcgc
      601 ccgatttaaa atcagccagc gcacgggtac gcgcaccttg cgatttatta ccgtggatcg
      661 ccgcactacg gatgccatct ttattaagct gttcagcgag atggttagcg ccatgtttgg
      721 tacgggtaaa caccagcacc tgctgccagt tccctttccc aatcatgtgc gacagcaatt
      781 cgcgtttgcg cttcttatca acaaagtgaa catgctgggt cacctgatca gacgcggtat
      841 tgcggcgtgc cacttcgatt tccagcgggt tgtgcaacag tttttccgcc agggctttaa
      901 tatcgtcaga gaaggtcgcg gagaacaaca ggttctggcg cttcgcaggt agttttgtta
      961 acacgcgacg gatatcgtgg ataaagccca tgtcgagcat gcggtcagct tcatcgagga
     1021 cgaggatttc aacctgatcc agcttcactg cattctgatg ttccaggtcc agcaaacgtc
     1081 ccggcgttgc caccagcaca tcaacgccgc cgcgcaactt catcatttgt gggttaatgc
     1141 tgacaccgcc aaacaccacc agcgaacgaa tgttcagata tttgctgtaa tcacggacgt
     1201 tttcgccaat ctgcgcagcc agttcacggg tcggggtaag aatgagagca cgtaccggac
     1261 gacgcccttt ggcgtgcggc tggcgagtga tcaggtgttg caacagcggc agcgtaaagc
     1321 ccgctgtttt gccggtgccg gtctgggcgc tagccatcag gtcgcggcct tccagcaccg
     1381 cagggatcgc ctgctgctga ataggggtgg gttcacggta accctgctcg gcaacggcgc
     1441 gcaggatatc agggcttaaa cccaaagaat cgaaagacat aactactccg aaccgccccg
     1501 accgttaccg gtgtagtttt cagggagata ctgaaaagga aaatgacaaa aaccacaatg
     1561 tcatgaggga gcggagtgta gcagtttttg tgacgcagcg cataaattat ccctgtgact
     1621 ggcgtagcta aaattcttag ccatactgga caactcccct atggagtcat aatcttaatc
     1681 aatcatttga ttaagaatgg actgtgcgat gaataatcct gccatgacaa tcaagggtga
     1741 acaggcgaaa aaacagctga ttgctgccgc actggcgcag tttggtgaat atggaatgaa
     1801 cgccaccacc cgcgagatag ccgcccaggc cgggcagaat atcgctgcca tcacctacta
     1861 cttcggttcg aaagaagatt tgtacctcgc ctgcgcccag tggattgccg attttattgg
     1921 cgagcagttc cgtccgcatg ccgaggaagc cgaacgcttg ttcgcccaac cacagcctga
     1981 tcgggctgcc atccgtgaac tgatccttcg cgcctgcagg aacatgatta agctgctcac
     2041 ccaggatgac accgtcaacc tcagcaagtt tatctcccgt gagcagctct ctcccacggc
     2101 agcctaccac ctggtgcacg aacaggtgat tagtccgcta cacagccacc tgacacgcct
     2161 gattgccgcc tggaccggct gcgacgccaa tgacacccgc atgatccttc atacccatgc
     2221 gttgattggt gagattctgg cgttccgtct tggcaaagaa acaattctgt tacgtaccgg
     2281 ctggaccgcg ttcgatgaag aaaagaccga actgatcaac cagacggtga cctgtcatat
     2341 cgacctgatt ttgcaaggat tatcgcaaag gagtttgtag tgatgaaaaa acctgtcgtg
     2401 atcggactgg cggtagtggc acttgccgcc gtggttgccg gaggctactg gtggtatcaa
     2461 agccgccagg ataacggcct gacgctgtat ggcaacgtgg atattcgtac ggtgaatctt
     2521 agtttccgtg ttggggggcg cgttgaatcg ctggcggtgg acgaaggtga tgcgatcaaa
     2581 gcgggccagg tgctgggcga actggatcac aagccgtatg agattgccct gatgcaggcg
     2641 aaagcgggcg tctcggtggc acaggcgcag tatgacctga tgcttgccgg gtatcgcgat
     2701 gaagaaatcg ctcaggccgc cgctgcggtg aaacaggcgc aagccgccta tgactatgcg
     2761 cagaacttct ataaccgcca gcaaggactg tggaaaagcc gcaccatttc ggcaaatgac
     2821 ctggaaaatg cccgctcctc gcgcgaccag gcacaggcaa cgctgaaatc agcacaggat
     2881 aaattgcgtc agtaccgttc cggtaaccgt gaacaggaca tcgcccaggc gaaagccagc
     2941 ctcgaacagg cgcaggcgca actggcgcag gcggagctga atttacagga ctcaacgttg
     3001 atagcaccgt ctgatggcac gctgttaacg cgcgcggtgg agccaggcac ggttctcaat
     3061 gaaggtggca cggtgtttac cgtttcacta acgcgtccgg tgtgggtgcg cgcttatgtt
     3121 gatgaacgga atcttgacca ggcccagccg gggcgcaaag tgctgcttta taccgatggt
     3181 cgcccggaca agccctatca cgggcagatt ggtttcgttt cgccgactgc tgaatttacc
     3241 ccgaaaaccg tcgaaacacc ggatctgcgt accgacctcg tctatcgcct gcgtattgtg
     3301 gtgaccgacg ccgatgatgc gttacgccag ggaatgccag tgacggtaca attcggtgac
     3361 gaggcaggac atgaatgatg ccgttatcac gctgaacggc ctggaaaaac gctttccggg
     3421 catggacaag cccgccgtcg cgccgctcga ttgcaccatt catgccggtt atgtgacggg
     3481 gctggtaggg ccagacggtg cgggtaaaac cacgctgatg cggatgctgg cgggattact
     3541 gaaacctgac agcggcagtg ccacggtgat tggctttgat ccgatcaaaa atgacggcgc
     3601 gctgcacgcc gtgctcggct atatgccgca gaaatttggt ctgtatgaag atctgacggt
     3661 gatggagaac ctcaatctgt acgcggattt gcgcagcgtc accggcgagg cacgtaagca
     3721 aacttttgct cgcctgctgg agtttacgtc tcttggcccg tttaccggac gcctggcggg
     3781 caagctctcc ggtgggatga aacaaaaact cggcctggcc tgtaccctgg tgggcgaacc
     3841 gaaagtgttg ctgctcgatg aacccggcgt cggcgttgac cctatctcac ggcgcgaact
     3901 gtggcagatg gtgcatgagc tggcgggcga agggatgtta atcctctgga gtacctcgta
     3961 tctcgacgaa gccgagcagt gccgtgacgt gttactgatg aacgaaggcg agttgctgta
     4021 tcagggagaa ccaaaagccc tgacacaaac catggccgga cgcagctttc tgatgaccag
     4081 tccacacgag ggcaaccgca aactgttgca acgcgccttg aaactgccgc aggtcagcga
     4141 cggcatgatt caggggaaat cggtacgtct gatcctcaaa aaagaggcca caccagacga
     4201 tattcgccat gccgacggga tgccggaaat caacatcaac gaaactacgc cgcgttttga
     4261 agatgcgttt attgatttgc tgggcggtgc cggaacctca gaatcgccgc tgggtgcaat
     4321 attgcatacg gtagaaggca cacccggcga gacggtgatc gaagcgaaag aactgaccaa
     4381 gaaatttggt gattttgccg ccaccgatca cgtcaacttt gccgttaaac gcggggagat
     4441 ttttggtttg ctggggccaa acggcgcggg caaatcgacc acctttaaga tgatgtgcgg
     4501 tttgctggtg ccgacttccg gccaggcgct ggtgctgggg atggatctga aagagagttc
     4561 cggtaaagcg cgccagcatc tcggctatat ggcgcaaaaa ttttcgctct acggcaacct
     4621 gacggtcgaa cagaatttac gctttttctc tggtgtgtat ggcttacgcg gtcgggcgca
     4681 gaacgaaaaa atctcccgta tgagcgaggc atttggcctg aaaagtattg cctcacacgc
     4741 caccgatgaa ctgccattag gttttaaaca gcggctggcg ctggcctgtt cgctgatgca
     4801 tgaaccggat attctgtttc tcgacgaacc gacttccggc gttgaccccc tcacccgccg
     4861 tgaattctgg ctgcacatca acagcatggt agagaaaggc gtcacggtga tggtcaccac
     4921 ccactttatg gatgaagcgg aatattgcga ccgcatcggc ctggtgtacc gcgggaaatt
     4981 aatcgccagc ggcacgccgg acgatttgaa agcacagtcg gctaacgatg agcaacccga
     5041 tcccaccatg gagcaagcct ttattcagtt gatccacgac tgggataagg agcatagcaa
     5101 tgagtaaccc gatcctttcc tggcgtcgcg tgcgggcgct gtgcgtcaaa gagacgcggc
     5161 agatcgttcg cgatccgagt agctggctga ttgcggtagt gatcccgctg ttgctgctgt
     5221 ttatttttgg ttacggcatt aacctcgact ccagcaagct gcgggtcggg attttactgg
     5281 aacagcgtag cgaagcggcg ctggatttca cccacactat gaccggttcg ccctacatcg
     5341 acgccaccat cagcgataac cgtcaggaac tgatcgccaa aatgcaggcg gggaaaattc
     5401 gcggtctggt ggttattccg gtggattttg cggaacagat ggagcgcgcc aacgccaccg
     5461 caccgattca ggtgatcacc gacggcagtg agccgaatac cgctaacttt gtacaggggt
     5521 atgtcgaagg gatctggcag atctggcaaa tgcagcgagc ggaggacaac gggcagactt
     5581 ttgaaccgct tattgatgta caaacccgct actggtttaa cccggcggcg attagccagc
     5641 acttcattat ccccggtgcg gtgaccatca tcatgacggt catcggcgcg attctcacct
     5701 cgctggtggt ggcacgagaa tgggaacgcg gcaccatgga ggctctgctc tctacggaga
     5761 ttacccgcac ggaactgctg ctgtgtaagc tgatccctta ttactttctc gggatgctgg
     5821 cgatgttgct gtgtatgctg gtgtcagtgt ttattctcgg cgtgccgtat cgcggttcgc
     5881 tgctgattct gttttttatc tccagcctgt ttttactcag taccctgggg atggggctgc
     5941 tgatttccac gattacccgc aaccagttca atgccgctca ggtcgccctg aacgccgcgt
     6001 tcctgccgtc gattatgctt tccggcttta tttttcagat cgacagtatg cccgcggtga
     6061 tccgcgcggt gacgtacatt attcccgctc gttatttcgt cagcaccctg caaagcctgt
     6121 tcctcgccgg gaatattcca gtggtgctgg tggtaaacgt gctgtttttg atcgcttcgg
     6181 cggtgatgtt tatcggcctg acgtggctga aaaccaaacg tcggctggat tagggagaag
     6241 agcatgtttc atcgcttatg gacgttaatc cgcaaagagt tgcagtcgtt gctgcgcgaa
     6301 ccacaaaccc gcgcgattct gattttaccc gtgctaattc aggtgatcct gttcccgttc
     6361 gccgccacgc tggaagtgac taacgccacc atcgccatct acgatgaaga taacggcgag
     6421 cattcggtgg aacttactca acgttttgcc cgcgccagcg cctttacaca tgtgctgctg
     6481 ctgaaaagcc cacaggagat ccgccccacc atcgacacac aaaaggcgtt actactggtg
     6541 cgtttcccgg ctgacttctc gcgcaaactg gatacctacc agaccgtgcc gttgcagttg
     6601 atcctcgacg ggcgtaactc caacagtgcg caaattgccg ccaactacct gcaacagatc
     6661 gtcaaaaatt atcagcagga actgctggaa ggaaaaccga aacccaacaa cagcgagctg
     6721 gtggtacgca actggtataa cccgaatctc gactacaaat ggtttgtggt gccgtcgctg
     6781 atcgccatga tcaccactat cggcgtaatg atcgtcactt cactttccgt cgcccgcgaa
     6841 cgtgaacaag gtacgctcga tcagctactg gtttcgccgc tcaccacctg gcagatcttc
     6901 atcggcaaag ccgtaccggc gttaattgtc gccaccttcc aggccaccat tgtgctggcg
     6961 attggtatct gggcgtatca aatccccttc gccggatcgc tggcgctgtt ctactttacg
     7021 atggtgattt atggtttatc gctggtggga ttcggtctgt tgatttcatc actctgctcg
     7081 acacaacagc aggcgtttat cggcgtgttt gtttttatga tgcccgccat tctcctttcc
     7141 ggttacgttt ctccggtgga aaacatgcca gtatggctgc aaaacctgac gtggattaac
     7201 cctattcgcc actttacgga cattaccaag cagatttatt tgaaggatgc gagtctggat
     7261 attgtgtgga atagtttgtg gccgctactg gtgataacgg ccacgacagg gtcagcggcg
     7321 tacgcgatgt ttagacgtaa ggtgatgtaa cttcttatct ttcgccagca aagacactac
     7381 cgccgggccg gcaaggattg ccagccctgc aattgccagc aaaaagttgt tttccagaac
     7441 gcgggacaac agccacagca caatcagcgc tgcgccaaaa taccaggtcg tggtcagctc
     7501 ttccagcacg tctgccacct cacgccagcg ttgctcgggg tggcgctgaa acaccagcat
     7561 catcactacc gtgacacaat acagtgcgca taatcccacg ccgacgtgaa ccagagtctt
     7621 actcatccag cccaccacca gcagcgccac taccagtaaa tgcaacataa tctgccagca
     7681 acttagaccc gttgcgacac gaacacgttg ttgccacttc atggcttctc tcctgaagaa
     7741 tttatctcta cttactcaga gtaccgaact ttacggaaaa ttccgtaaga ccgcaccttt
     7801 ttgtgacgcc gactacacta tttcaatgag ataagaggca aagacaggaa aattatgccc
     7861 gatcaaacac aacaattttc gttcaaggtg ctcaccatca atattcacaa aggctttacc
     7921 gcgtttaacc gacgcttcat tttgccggaa cttcgcgatg ccgtgcgtac cgtcagcgcc
     7981 gatattgttt gcctgcagga agtgatgggc gcgcacgaag ttcatccgct gcatgtggaa
     8041 aactggcccg atacctcgca ctacgagttt ctcgccgaca ctatgtggag cgattttgcc
     8101 tacggtcgca atgccgtata cccggaaggg catcacggca acgccgtact gtcgcgttat
     8161 cccattgaac attatgagaa tcgcgatgtt tcggtcgatg gtgcggaaaa gcgcggcgtg
     8221 ctctactgcc gcattgtgcc gccgatgacc ggaaaagcga ttcatgtgat gtgcgtacat
     8281 ctgggcctgc gtgaggcgca ccgtcaggcg cagcttgcga tgctcgccga atgggtgaat
     8341 gagctaccgg acggcgaacc ggtattggtg gcgggtgatt tcaatgactg gcggcaaaaa
     8401 gctaatcatc cgttaaaagt gcaggccgga ctggatgaga tttttacccg cgcccacgga
     8461 cgcccggcgc gcacgtttcc ggtgcaattt cctctactac gactggacag gatctacgtc
     8521 aaaaatgcca gcgccagcgc gccaaccgcg ttgccgctgc ggacatggcg acacctttct
     8581 gatcatgccc ctttaagtgc ggagattcat ttatgaaatg tagctggcgc gaaggcaata
     8641 agatccagtt gctggaaaac ggcgagcaat attatcccgc ggtgtttaag gcgattggcg
     8701 aggcacaaga acgcatcatt cttgaaacgt ttatctggtt tgaggatgac gtcggcaaac
     8761 aactgcatgc ggcactactg gcagcagcgc aacgcggggt taaagcggaa gtcttgctgg
     8821 atggctacgg ttcgccggat ctcagcgatg agtttgtcaa tgaactgacg gcagctggcg
     8881 tggtgttccg ctactacgat ccccgccctc gcctttttgg tatgcgcacc aatgtgtttc
     8941 gccggatgca tcgcaaaatt gtggtgatcg atgcgcgtat cgcctttatt ggcgggctga
     9001 attattccgc cgagcatatg tccagctacg gtccagaggc caaacaggat tacgcggtgc
     9061 gccttgaagg gccgattgtc gaagatattc tccagtttga gctggaaaac ctgcctggac
     9121 agagcgcggc acgacgctgg tggcgacgtc atcacaaagc ggaagagaac cgccagccgg
     9181 gagaagcgca ggtattgctg gtctggcgcg ataacgaaga acatcgcgat gatattgaac
     9241 gtcactatct gaaaatgctc actcaggcgc gtcgggaagt gattattgcc aacgcctact
     9301 tcttccccgg ctatcgattt ttacacgcct tgcgtaaagc ggcacggcgc ggggtgcgga
     9361 tcaaactgat cattcagggc gaaccggata tgccgattgt cagagtcggt gcgcgcttgc
     9421 tgtataacta tctggttaaa ggcggcgttc aggtgtttga gtaccgccgc cgcccgctcc
     9481 acggcaaagt ggcattgatg gacgatcact gggcgacagt agggtccagt aatctcgatc
     9541 cgctcagttt gtcactgaat ctcgaagcaa atgtcatcat ccacgatcgt cattttaacc
     9601 agacgctgcg cgataatctg aacggcatta ttgccgcaga ttgtcagcag gtcgatgaga
     9661 ccatgctgcc gaaacgcacc tggtggaacc taaccaaaag cgtgctggcg ttccactttt
     9721 tacgccactt cccggcgctg gttggctggc ttccggcaca cacgccacgt ctggcgcagg
     9781 ttgatccccc cgcacaaccg acaatggaaa cgcaggatcg ggtagaaact gaaaacacgg
     9841 gggtaaaacc ctgatgagta aatcacaccc gcgctggcgc ttagcaaaga agatcctcac
     9901 ctggctgttt tttatcgcgg tgattgtgtt gctggtggtc tacgccaaaa aagtggactg
     9961 ggaagaggtc tggaaggtca tccgcgacta caatcgcgtt gcgctgctta gtgcggtcgg
    10021 gctggtggtc gtcagctatc tgatttacgg ctgctatgac ctgctcgccc gcttttactg
    10081 cggtcacaaa ctggcgaagc gccaggtgat gctggtgtcg tttatctgct acgccttcaa
    10141 cctgacgctc agtacctggg tcggcggcat tggtatgcgc tatcgtttgt actctcgtct
    10201 ggggttgccc ggtagcacta ttacgcggat tttctcgctc agtattacca ccaactggct
    10261 gggctatatt ttgctggcgg ggattatctt taccgcaggc gtggtggagc taccggatca
    10321 ctggtatgtc gatcaaacca cactgcgcat tctcggcatt ggcttactga tgattatcgc
    10381 ggtttatttg tggttttgcg ctttcgcgaa gcaccgccat atgaccatca aagggcaaaa
    10441 gctggtgctg ccctcatgga aattcgccct cgcccagatg ttgatttcca gtgttaactg
    10501 gatggtcatg ggagcgatta tctggctgtt acttggtcaa agcgtgaact atttctttgt
    10561 actgggcgtg ttgctggtta gtagtattgc aggcgtcatc gtgcatattc cggcggggat
    10621 tggtgtgctg gaagcggtat ttatcgcatt actggcaggg gagaatacat ccaaaggcac
    10681 aattatcgcc gccctgctcg cttatcgcgt cctgtattac tttatcccgc tgctgctggc
    10741 gctgatctgt tacctgctac tggaaagcca ggcgaagaag ctacgggcga aaaatgaagc
    10801 ggcgatgtga agttcagtta agttctttag tatgtgcatt tacgactaat gaaaaaaacg
    10861 cgtatgcctt tgccagacaa gcgttatagc tatcgcggca agtatcccaa agaagccgat
    10921 aaaatagtaa tagagtgtta atgcacctat aaaagcgcaa cgaaccggac tccacaactc
    10981 ttcactatgg catttccccg ccagttcaag gagcttattt cgtccatgcg agataatgcc
    11041 tgaccataac acaatcatta agcaacttat tacccaaatg ggccgttcac tcattaatgc
    11101 cgcattcacg atgattacca gggccaaccc tgtcaacgtc atcataatga taaaacggtg
    11161 agaaccagga tcaacattaa ataataaacc ggccagcata ctgattaaaa acatcgcgcc
    11221 cgcagtgctg aacacgatga taatggcata tacaggaact aaacttgcaa cgacaatcgc
    11281 caccaaagag agtaaaaaca gatatattac gcctgcgatg gcggcattta tcttgttgat
    11341 atccttactt aagccataca tacaaagtaa taataaccct atggcgacat acattggtgg
    11401 tgaatcatat ttggttgccg taatttcgag atacttagca ccccaggcca gccctgcggt
    11461 tgccaccaaa cctaaagcgg taatgagcca tataccgttt aatatcctgg tctcgtgctg
    11521 aaaatctaat ttattgctgt tttgcaagta tgattccatt cttttagttg tccttataga
    11581 aaaatattaa tattcatcat gaataaattt aaacccacgc atacaaaata cttttgtatt
    11641 tatggatacg gaaaacgctt ccgctcggcc ataaggcata ttttatacct ggtacttgtt
    11701 aagtgtcgtt tttagtaaga aaaaaaatcc cggcaccagg ccgggatcag caggaagaag
    11761 agaattaacg tcgattgcca aaaattcgca gcaacatcag gaacaggttg atgaagtcca
    11821 gatacaaggt taacgcgcca agaatggaat atttgcgcag gttcgacgcg tcgcgggtat
    11881 ctatctgctc acccatattt ttcagtttct gggtgtcata cgccgtcaat ccgacaaaga
    11941 caatcacgcc gatgtaggta actgcccaca tcaatgcttc gcttttcagc cagaagttga
    12001 ccagcgatgc cagcacaatg ccgattaacg ccataaacag catattgcca aagccactta
    12061 aatcgcgctt cgtggtgtaa ccgtacaggc tcattgcgcc gaacatcccg gcagtaacga
    12121 cgaaggtact ggcgatagaa gcagcggtat aaacaatgaa aatactggaa agcgtaagac
    12181 ccgtcagcgc cgaataaagc ataaagagca tcgtcgttac acctgcgctc agcttttgaa
    12241 tcatcgctga taacacaata accaatgcta attgcgcgat gatcagaccg attaaaaaga
    12301 cacggttagt gaacaacagc tccatcacgg ccgcggaatt agccgcatac caggcaacaa
    12361 atgcggtcag caacaagcca acggtcatcc agccatagac ttgcgccata taagtctgca
    12421 agccagcccg gggttgtaca attgaatcag aacgtgggaa tctgtccatg acgatctcct
    12481 gaagatataa ggaatatctt aaggatactg caaaatgatg aggctgtgca tcgacgcagc
    12541 gtaaacgcat gtactgagcg gtgaaattgc cggacgcagc ggtgccttat ccggctaaca
    12601 aaaactacca gcgttttgcc gcctgctgat cgctctcccg agcttcgacc cagcggtcgc
    12661 cctccggcgt ggcttcgcgc ttccagaacg gtgcgcgggt tttgagataa tccataataa
    12721 actgcccggc ttcaaacgca ctgctgcgat gcgcactggt gacaccgaca aaaacgattt
    12781 catcgcccgg ccataattcc ccgatgcggt gaatcaccgt gacgcgcccc agcggccagc
    12841 ggttacgcgc ttcatcgaca atttctgcca gtgctttttc agtcatcccc ggatagtgtt
    12901 cgagggttaa tgctttgacg ctgtcgccca ggttatggtt gcgcacctta ccggtaaagg
    12961 tgactaccgc accgtcttcg tcacgctccg ccagccacgg gtactcttct cctacgctga
    13021 acggctgcgg accaacaaca attttggttt ctgccatctt aacctccggt taccggcggg
    13081 aagaaagcta cttcgtcgcc gtcagtcagc ggatggtcaa aactcaccag cgtctggttg
    13141 acggcagcca gtaatttgcc atcttccagc gccagcgccc agcgatcgct ctgcgcagcc
    13201 aggtgctggc gtaacgcttc aacggttggg aaatccgcag ccacttcggt tgcatctgtt
    13261 cctaccaact cgcgcacctg ggcgaaaaaa agaactttaa tcatccgctt ccaccttaaa
    13321 gtcacccgac ttgccgccgc tcttcgccag caaacgtacc ggaccaatca ccatatcttt
    13381 ttgcaccgct ttgcacatgt cataaatggt cagcgccgcc acggaggccg cggttaacgc
    13441 ttccatttcg acaccggttt tcccggtcag gcggcataag gtttctatac gcacccgatt
    13501 gtgctccggc tcggcctgta aattgacttc aactttgctg agcatcagcg gatgacacag
    13561 cgggatcaga tcccaggtgc gttttgccgc ctgaataccg gcaatacgcg cagtggcaaa
    13621 tacgtcgcct ttgtggtggc gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt
    13681 gacaaaggct tccgcccgcg cttcacgcac ggtttccgct ttggcggaga catccaccat
    13741 gtgcgcttcg ccagcggcgt tgatatgggt cagttgcgac atacttattt cttcaaatgt
    13801 ggatggaaat tacacggacg cgtacgggca tccagctgcg gcgcgatgat attttcccat
    13861 gcggtacggc acgctttggt cgaacccggc atggcgaaaa tcagcgtttt gttagcgacg
    13921 cccgctaccg cacgagattg caacgtggaa gtgccaatct cttcaaacga caacatacgg
    13981 aacacttcac caaaaccttc aacttcacgg tcgaacaacg gcagcaatgc ttcgggagcc
    14041 tgatcacctt ccgtcaggcc agtccccccc gtaatcaaca ccacttgtac atcgtcgctg
    14101 gcgatccacg cagatacctg agcgcgaata gcgtagcggt tttctttcac aatggcttta
    14161 tcgacaacgt gatggcccgc ttcttgcgcc gaatcgcgca aatagtgacc ggaggtatcg
    14221 tcttcttcac cgcgacgatt agaaaccgta agaatagcaa tacgggtcgg gataaattca
    14281 gtgcttacct gactcatctg atctctcctt ttgacgtttt agccgccaat gtacgataag
    14341 ttttgcgtaa taccggtgtt gttttgatgc aggaaatggg tctgtttctt ctcccgcagc
    14401 gccgctgaaa tacgcgcttc cagcgcctgt tgctgggtat cgtcttccag cagatcgcgc
    14461 aggttaacgc cgccttcacc aaacaggcag agatggagtt taccaatgga ggaaacgcgc
    14521 agacggttgc aagtggcgca gaagtctttt tcatacggca tgataaggcc aatctctccg
    14581 gcgtaatccg gatgacaaaa gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg
    14641 atccagccgc gacgcagtag ctcgtcacgc agaacctgac cagagatgtg atgcttacgg
    14701 aagagctcgc tgccctcgcc cgtttccatc agttcgatga aacgcagctg gatagggcga
    14761 tgctggatcc agttcagaaa ggtgtcgagc tggtgatgat taacatcacg catcagcacg
    14821 gtattgactt tgaccttctc aaaaccggcc tcaaatgcag catcaatccc tgccatcacc
    14881 tggttgaatt tatcctgccc ggtaatagcg tgaaactggc gggcgtccag actatcaacg
    14941 ctgacgttga tgccagtaag tccagcatcg cgccagttcg ccacatcgcg ttccagacgg
    15001 taaccattgg tggtgaccgc tatctggcga atagcgtcgt tttctcgcac agcggcgatg
    15061 atatcggtaa agtcgcggcg taaagacggc tcaccgcctg tcaggcgcac tttttcggtg
    15121 cccagactgg cgaaggcgcg cgtaacccgg cgaatttcat cgacggtcag aaagccttta
    15181 ttggtgacgc cgctcggttt gtagccatcc ggcaggcagt aggtgcaacg aaagttacac
    15241 acatcggtaa tcgacaggcg caagtagtaa aacttacgcg caaatgcatc agtcagttgt
    15301 gaagccatgt acacctttcc agatacggga ggcgaagtca tttcttcctt cgccctggtg
    15361 gcaatcttta cgagcattgt cacggccaaa gcaccatatc agttgaccca ggtgcagagg
    15421 ctagagtgtt tagtggttaa gccgatacta gcgtgtaaat gagattttta ccatccacac
    15481 tttcgctatg taatcatgta tatagcgtca tgatcgtgct atttcgctac agaataaggg
    15541 aaaatggcgg ttttactttg atatacatca tcagggaggc atagcgtgca tcgtccttta
    15601 gaggtagaag aaaatagttt gtatcctcaa tattggcagg ttaattgctg tttccccgca
    15661 atttgcgcta ctgtagcgcg gataatctga ctccaggaaa ctatatgcgc aatcgtacgc
    15721 tggctgatct tgatcgtgtc gttgctctcg gcggagggca tggactggga cgcgttctct
    15781 catcactttc gtctttgggt tctcgtttaa cgggtatcgt caccaccacg gataatggtg
    15841 gctcgacggg gcgtattcgc cgttcagaag gcggcattgc ctggggcgat atgcgcaact
    15901 gccttaacca gctgataact gaacccagcg tcgcttccgc aatgtttgaa tatcgtttcg
    15961 gcggtaatgg cgaactttcc gggcacaacc ttggaaacct gatgttgaag gcactggatc
    16021 accttagcgt gcgacctctt gaagcgatca atctgattcg aaatctgctg aaagtggacg
    16081 cacatttgat tccgatgtcg gagcatcctg ttgatctgat ggcgattgac gatcaggggc
    16141 atgaagttta cggcgaggtc aatatcgacc agttaactac gccgattcaa gagttattgt
    16201 taacgcctaa tgtacccgca acgcgtgagg cggttcacgc tatcaatgaa gcggatctca
    16261 tcattattgg gcctggcagt ttttatacca gcctgatgcc cattctgctg ctgaaggaaa
    16321 tcgcgcaggc attacgccgc acgccagcgc cgatggtcta tatcggcaat ctggggcgtg
    16381 agttgagttt acctgcggcc aatttgaagc tggaaagcaa gctggcaatt atggagcagt
    16441 atgttggtaa aaaggtcatt gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg
    16501 tgaaagagcg gattgtgatt caggaggtgc tggaggccag cgatattccg tatcgtcatg
    16561 atcgccagtt gttacatagc gcactggaaa aggcgttaca ggctttaggt taacggaaat
    16621 ccgaaggaaa attccggctt cctattgaag acaaagtgcg cgttgtttat gccggatgcg
    16681 gcgtgaacgc cttatccggc ctacaaaccg cgctaattca atgtattgca gaatcatgta
    16741 ggcctgataa gcgtagcaca tcaggcaatt ttgcttttgt catctgtctt cttcgttatc
    16801 ctgtcacgaa gccgcgataa acaattcacg cagctgatgc aactggtcac gaatttgtgc
    16861 cgcttcttcg aactccagat tctgcgcgtg ttgcatcatt aacccttcca gctcatggat
    16921 tttctgttgc aacgctttag gcgacatatc catcggcaca ttatccggct caacaatcgg
    16981 gcgcgatttt cctctgccct tcgctttggt tttggcgatg ttctgcccca gcgccaggat
    17041 atcgaccact ttcttgttca agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg
    17101 ctgtttctcg cggcgacgtt cggtttcgcc aatcgctttc gccattgatg gggtgatctt
    17161 atcgccgtag agaatcgctt taccgttaac gttacgtgcc gcacgaccaa tggtctggat
    17221 caacgaacgt tcggaacgca ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga
    17281 aacctccggc atatccagac cttcgcgcag taagttgatc cccaccagca cgtcgaactc
    17341 acccagacgc aagtcgcgga taatctccat acgttcgacg gtgtcgatat ccgagtgaag
    17401 ataacgcacg cgctcgccat gttcttcgag atattcggtg agatcttccg ccatccgctt
    17461 ggtcagtgtg gtgaccagta cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag
    17521 aagatcatca acctgtgtcg ccactggccg cacttcgata atcggatcaa gcaatccggt
    17581 tggacgcacc acctgatcca ccacatcgcc gccggatttt tccagctcat aattacctgg
    17641 cgtcgccgaa acatagatgg tttgcggcgc taatgcttcg aactcttcaa atttcagcgg
    17701 acggttatcc agcgctgatg gcaggcggaa gccgtactcc accagtgtct ctttacgcgc
    17761 ccggtcaccg cgatacatgc cgccaatttg tggaatggtg acgtgagatt catcgaccac
    17821 cagcagccca tcggcaggca ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg
    17881 accagagagg aagcgcgagt agttttcaat ccccgaacag tagcccagct cgttcatcat
    17941 ctccagatca aactgggtac gctgggtcag ccgctgctct tccagcagtt tgttgttttc
    18001 caacagcact ttgcgtctgg cggccagctc ttctttgatc tcctccatcg cctgtacgat
    18061 gcgctcgcgc ggtgtgacgt agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga
    18121 aacaatctgc ccggtcagcg ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag
    18181 ttccacgcga agtgcaatgt catccgattc tgccgggaat atatcaatca cctcgccacg
    18241 aacgcggaaa gtaccgcgct ggaatgcctg atcattacga gcgtattgca gctccgccag
    18301 tcggcgcaga atcgcgcgct gatcgataat cataccgacc gtgagatgga gcatcatctt
    18361 gagatataaa tcaggatcgc ccagaccata aatcgcggaa acagacgcca ccacaaccac
    18421 atcacgtcgc tccagcatcg ctttggtggc ggacaaacgc atctgttcga tatgttcgtt
    18481 taccgaggca tctttctcaa tgaaggtgtc agaactcggt acataggctt ccggctgata
    18541 gtagtcgtag taggagacga aatattccac cgcgttttcc gggaagaact ctttcatttc
    18601 gccatacagc tgggccgcca gcgttttgtt gggcgcaagt accatggtag ggcgctgaag
    18661 gtcagcaatg acattggcaa tggtgaaggt tttcccggag ccagtcacgc caagtaacgt
    18721 ctggtgcgcc aggccatctt ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc
    18781 gccagaaggt ttaaaagcgg aattcagttt gaacggttta ctcatgagtc tctacctgaa
    18841 ggagttgggc gggcaggtat gtaattttac tcgtcgtact taattatgcc aacaaattat
    18901 actggataaa aaaacagttc atcaccataa tatttctgat acagcgtaaa ctccgctgtc
    18961 aatcatgagc aaaatttact ctgtggcgag ataaaactcc ggccttaccg ggttatcccc
    19021 aaagcaacgg cttttttaac atttgtcaag atgagtggtg acagttttat ggcaagagat
    19081 gcctcgttag tgactgccat tgcaattatc taaccagtta aaaaataaaa gaaatttctt
    19141 tgagccgtct ttaacgccaa ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt
    19201 ttctaactct aatacacatg gttatccaca ggaatagtgg ataactgtct gcagccccta
    19261 tcccccgccg cctgggcaat tcccacattc gccgcatgat gcggaagtaa aatttttttc
    19321 gtgattattt tagaatttaa ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg
    19381 caatttaacc aaacacagga tggctacaac aaggcaaggt ttatgtactt tccggttgcc
    19441 gcattttctg gattttctgc aagcctgggg atctctccca gcagcggcgc gggaatcatg
    19501 cgggtgagcg tggtcatata ttcagcgtga cgttttcccg gaggcgtgac atcgttcgcc
    19561 acccaacccg ccagagtcag cccggcgtgt tgtatcgcct gtgccgtcaa catcgcgtga
    19621 ttaatacagc caagtttcac gccaaccacc agtattaccg gcagttgttc ctgtgttacc
    19681 caatccgcaa aagtgaaagt gtcagaaagc ggcgtaaacc agccgccagc gccttccact
    19741 aacacccagt cagcctgttg ttcaagcgcg cgtaatccgg agctcattac cgatgattct
    19801 atcgacctgc cctcttgcgc gctgatgatg tgcggtgagg tgggttctgc gaaggtgtaa
    19861 ggatttaccg ttgcgtaatc cagctgcagg ctgctgttgc gctgtaacgc cagcgcgtca
    19921 ctattacgta aaccctccgg ggtcttttcg ctgccagagg cgaccggttt ataacctgcc
    19981 gtccgatagc ctgctgcctt tgcggcttgt aaaagtgcac aactggcgac agttttcccc
    20041 acttcggtat ccgttccggt gacaaaataa cgtttactca cgaacaatca ctcccaaaaa
    20101 aagatgatac gtcagaggat atcgcccctg ttgttgcggc caggccagtt gcaatcgctg
    20161 caactgtgaa cgcgttaata ttcgctggtc gcgcccttca tgaagatgcg tggcaccgat
    20221 gcctttcagc gaacgcatgg cactgagcgc atcatcaaac cacagcgtga tgggctgaat
    20281 atgatgtcga taatgcacgc cgttcagtga ctgttcgatt tcatctggcg gtaaaaagcg
    20341 attagcatgc gggcgctcgt ccaccgcctg ccacgcctga tgcagttcgg gtaacgatcc
    20401 ctgcaccagc gtggtaaacg cgaccacgcc tccggggcgc accacccgat acagctcgcg
    20461 gagtgccgta gataaattac cgcaccactg caccgcgaga ttgctccata caagatcgaa
    20521 cgtcgcagtc gctaacggca gggattcgat atctcccgcc agataatgat ctgcggcatc
    20581 tttatggcgg gcttgcgcaa gcattggcgg cgagagatct aacgccgtca cctgcgcgtg
    20641 acgctctcgc cagtggcggc tcatccagcc tggcccacac cccgcgtcca gtacgtgggt
    20701 gtatttacgc tgtgggagca ttgccagtaa gacgtcagca ctctggcgct gtaactctgc
    20761 gtgttgctca tagtgcgcgg ctgcccgacc aaatgccgct gcaatggctt gtttattaac
    20821 cgttgccatg cagcacctcc agcagacggt cgatatcctg catttcatgc gcggaggtta
    20881 gtgttaagcg cagtcgcgca gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc
    20941 agcagccttg ctggcgcagt ttttctgcca gttgtaacgc acggctgtta tcaccgacga
    21001 tcaatggctg gatggcgctg catgaatcag caagcgtaaa cggcaaatcc tgtactccgg
    21061 cacgaaaacg cgtaatgagt gccgccagtt tttcgcgccg tgcatcaccc tcatcactgc
    21121 gaatgaccgc cagtgacgca cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt
    21181 agataagatg gcgggcgaat tgcagcagat aatccgccac cgtactggag caaagcaccg
    21241 cagccccgct gacgccaaat cctttgccaa aagtcactac cagcaattct ggttttacct
    21301 tttgcaacca gcaggtgccg cgcccctgct ccccgataac gcccgtgccg tgggcatcat
    21361 cgaccatcaa ccagccattg tgctgttgcg ttacctgctg gatttccgcc agtggcgcac
    21421 tatcgccgtc catgctgaac acgccttctg tcaccaccag ttgctgcccc gaacaggggg
    21481 aagcaagcag tcgcgccaga tgagcgacat cgttatggat aaaacggcga agctgcgacg
    21541 ggcttaaact ggcagcctcc agcaatgagg catggctaag ccggtcggca acaatacggt
    21601 cctctttcgc catcatcgcg gcaatgactg cctgattcgc ggcaaaacca gagataaaca
    21661 gcagcgcccg cgaatagcca agccactcgg ccagttcttc ttccagcgcc tgatgcgcca
    21721 cgctataacc gctgacatgc ccggaaccgc cgctacctac accaaacttt tctgcgccct
    21781 gctgccaggc gcggataatt tgcggatgat ggcttaaacc taaataatcg ttactggaaa
    21841 agttcagata ctggcgatca tccgccacca gccagcgtcc ggctccttgc gtcaccggat
    21901 aacggcgacg cagggcatcg gcagcacgcc gcgcatcgag cgccgcgttg attttctcct
    21961 gccagctcat agtgctgccg cgttgtaata ttcgtcagta tccggggtca tcagtgcctg
    22021 ctccagacgc tgctgttgtt cgttatcccc tgccagcacg gcagtttgct gcggatttaa
    22081 ccccagtttg cgaaacagtt gcaggtcttt atcttcttcc ggattcggcg tagtcagcag
    22141 tttgcagccg tagaaaatcg agtttgcgcc agccataaag cacatcgcct gagtctgttc
    22201 gttcatctgc tcgcgtccgg cagaaaggcg tacgtaagag gtcggcatca tgatccgcgc
    22261 tacggcaatg gtgcgaataa aatcaaaggc atcaacgtca tcgttatcgg caagcggcgt
    22321 gcctttcacc ttcaccagca tgttgattgg cacgctttcc ggcggcgtcg gcaggtttgc
    22381 cagttgcagt aataatccgg cgcgatcttt taccgtttcg cctaaaccca caatgccacc
    22441 tgagcagact ttgatcccgg catcgcgcac tttttccagc gtatcaaggc gttcctgata
    22501 agtacgtgtg gtgatgatat tgccgtaaaa ctccggcgag gtgtcgaggt tatggttgta
    22561 gtaatccagc ccggcattcg caaggcgctg cgcttgagat tcactcaacg tgcccagcgt
    22621 catacacgcc tccagcccca tcgcttttac cccctgcacc atttgttcca ggtacggcat
    22681 atcgcgttcg tggggattct tccacgccgc gcccatacag aaacgcgtcg atcctgccgc
    22741 tttcgctttg cgcgccgact ccagcacctg ttcaacttcc atcaacctct cggcttccag
    22801 cccggttttg tagcgcgagc tttgcgggca gtatttgcaa tcttccggac aagctccggt
    22861 cttaatcgac agcaacgtgc tgacctgcac ctgacgagga tcgaaatgct ggcgatggac
    22921 ctgctgcgct tcaaacagca gatccagcaa cggtttttca aataattctg tgacttgcga
    22981 caatgtccag cgtgggcggt gagccatggg gcttctccaa aacgtgtttt ttgttgttaa
    23041 ttcggtgtag acttgtaaac ctaaatcttt tcaatttggt ttacaagtcg attatgacaa
    23101 cggacgatct tgcctttgac caacgccata tctggcaccc atacacatcc atgacctccc
    23161 cgttaccggt ttatccggtg gcaagcgccg aaggttgcga gctaattttg tctgatggca
    23221 gacgcctggt tgacggtatg tcgtcctggt gggcggcgat ccacggctac aatcacccgc
    23281 agcttaatgc ggcgatgaag acgcaaattg atgccatgtc gcatgtgatg tttggcggta
    23341 tcactcatgc gccagccatt gagctgtgcc gcaaactggt ggcgatgacg ccgcaaccgc
    23401 tggagtgcgt ttttctcgcg gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg
    23461 cgttgcagta ctggcaagcc aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg
    23521 gttatcatgg cgataccttt ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca
    23581 gcctgtggaa aggctatctg ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg
    23641 atggtgaatg ggatgagcgc gatatggtgg gctttgcccg cctgatggcg gcgcatcgtc
    23701 atgaaatcgc agcggtgatc attgagccga ttgtccaggg cgcaggcggg atgcgcatgt
    23761 accatccgga atggttaaaa cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga
    23821 ttgccgacga gatcgccacc ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg
    23881 cagaaatcgc gccggacatt ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc
    23941 tttccgccac actcaccacg cgcgaggttg cagaaaccat cagtaacggc gaagccggct
    24001 gctttatgca tgggccaact tttatgggca atccgctggc ctgcgcagca gcaaacgcca
    24061 gcctggcgat tctcgaatct ggcgactggc agcatcaggt agcggctatt gaagcgcagc
    24121 tgcgcgagca acttgccccc gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg
    24181 gggccattgg cgtggtcgaa accactcgcc cggtgaatat ggcggcgctg caaaaattct
    24241 ttgtcgaaca gggtgtctgg atccggcctt ttggcaaact gatttacctg atgccgccct
    24301 atattattca cccgcaacag ttgcagcgtc tgaccgcagg ggttaatcgt gcggtacagg
    24361 atgaaacatt tttttgccaa taacgggaag tcagcgtgag ggtttctggc tacactttct
    24421 gcaaactaga aaggagggtt tatgaaactc atcagtaacg atctgcgcga tggcgataag
    24481 ttgccgcatc gtcatgtctt taacggcatg ggttacgatg gcgataatat ttcaccgcat
    24541 ctggcgtggg atgatgttcc tgcgggaacg aaaagttttg ttgtcacctg ctacgacccg
    24601 gatgcgccaa ccggctccgg ctggtggcac tgggtagttg ttaatttacc cgctgatacc
    24661 cgcgtattac cgcaagggtt tggctctggt ctggtagcaa tgccagacgg cgttttgcag
    24721 acgcgtaccg attttggtaa aaccggatac gatggcgcag cgccgccgaa aggcgaaacc
    24781 catcgctaca tttttaccgt tcacgcgctg gatgtagagc gtattgatgt cgatgaagga
    24841 gccagcggcg cgatggtcgg gtttaacgtt catttccact ctctggcaag cgcatcgatt
    24901 accgcgatgt tcagttaatt actctgccag atggcgcaat gctatctggt atcacttaaa
    24961 ggtattaaaa acaacttttt gtctttttac cttcccgttt cgctcaagtt agtataaaaa
    25021 agcaggcttc aacggattca tttttctatt tcatagcccg gagcaacctg tgaacacatt
    25081 ttcagtttcc cgtctggcgc tggcattggc ttttggcgtg acgctgaccg cctgtagctc
    25141 aacaccaccc gatcaacgtc cttctgatca aactgcgcct ggtacctctt ctcgcccgat
    25201 tctgtcggca aaagaagcgc agaatttcga tgctcaacac tattttgcat ccctgacacc
    25261 aggtgctgca gcgtggaatc cgtccccgat aaccctgcct gcgcaacctg actttgttgt
    25321 cggcccggcg ggtactcaag gtgtaacgca taccacgatt caggcggcgg tagatgcggc
    25381 aattatcaag cgcaccaaca agcgccagta tattgccgtg atgcctggtg agtatcaggg
    25441 aacggtgtat gtccctgctg ctccaggtgg aattactctg tacggtacag gtgaaaaacc
    25501 gattgatgtg aagattgggc tttcccttga tgggggcatg agtcctgccg actggcgtca
    25561 cgacgtcaac ccgcgcggca aatatatgcc aggtaaacca gcgtggtata tgtacgatag
    25621 ctgccagagc aaacgcagcg acagtatcgg tgttctgtgt tctgcggtct tctggtcaca
    25681 aaacaatggc ctgcaactgc aaaacctgac catcgaaaac acgctgggtg atagcgtaga
    25741 tgcgggtaac catccggcgg tggcactgcg tactgacggt gaccaggtac agattaacaa
    25801 cgttaacatt ctcggtcgtc agaacacctt ctttgtcacc aacagcggtg tgcagaaccg
    25861 tctggaaacg aatcgtcagc cgcgtacgct ggtgaccaac agctacattg aaggggatgt
    25921 ggatatcgtt tctggtcgcg gcgcagtggt gttcgataac accgaattcc gcgtggtgaa
    25981 ctcacgtact cagcaagaag cgtatgtgtt tgcaccggct acgctgtcca acatttacta
    26041 cggtttcctc gccgtaaaca gccgtttcaa tgcttccggt gatggcgtgg cacaactggg
    26101 ccgctcgctg gatgttgatg ccaataccaa cggtcaggtg gtgatccgtg atagcgccat
    26161 caacgaaggt tttaacacgg ctaaaccgtg gggcgatgcg gtgatctcta atcgtccgtt
    26221 tgcgggtaac accggcagcg ttgacgatag cgacgaaata cagcgcaatc tgaatgacac
    26281 taactacaac cgcatgtggg aatacaataa ccgcggcgtg ggtagcaaag tggttgcaga
    26341 ggcgaagaag taagagctat taactatttg ccggatgcgg cgtaaacgcc ttatccggcc
    26401 tacggttcga tgcgatttgt aggtcggata agatgcgcca gcatcgcatc cgacaataag
    26461 tgccgaatgc gacctacatt cacatggcgc tttttacatc tgacggtttt tattgaagtt
    26521 aatcaaacta cccgccttga taatctcgcg ctcttcagca gtcagacttt ccatatagag
    26581 cgtaatttcc gttaccggcg catcttcatg gatcacataa cctttaaacg tcgtacccgg
    26641 attatccagc gccgctttaa tgccagggat gtaaatgtaa tcccccactt caaaggttgg
    26701 cacttccgcc atctgcagag gtaacatccc ccagttgatg acgttagaac gatagcgttt
    26761 agtcgcgtac tcctcggcaa tattcgccag accgccaatc acacgctgac aactcgccgc
    26821 ctgttcacgc gcagaaccat cgcctggttt caccgcatag accatgctgc caatttcagt
    26881 ttgcagcgga tcaatatgct cctgaccagc aatctgctta atgcgcgcaa acacctctgt
    26941 cagctcgctg acattccccg ccagacgctg attttccagc tcagcagtcg ctttacttct
    27001 gccaacataa cctggatcgc ggcgtgacag ggtaaactcc gccagaccaa tcggatttga
    27061 acgataagaa gaggtttcac cggaagggat cagttcgtcg gtggtggtca cttcgtcgag
    27121 gatcttcgag cacactttga ggacgatatt gtcagtcagc gcacctaatt ccggccagtc
    27181 tttaatgttc ggtccgtaaa tcagcggttg ctgagttgcc cctttaacaa agccctgata
    27241 aacacggttt ttatacggcg ttacatcgaa ggcgtactcc ggcacgttgt cccagcaatc
    27301 gagttcgctg gcagaagtta aatagccacc gtttgccgca gtcgcagcga tagaacgagc
    27361 gtccatcaac gccaccgctg acatctgccc attggctggc ttagaacctt cgcggttcgg
    27421 gaagttacgc gtggtgtggc gaatactcaa accgttgttg attggcgtat cgcccgcgcc
    27481 aaagcatggg ccgcagaacg cggttctgat gattgcgcct gcgccaatca aatctgctac
    27541 cacacctttt ttggctaaat ccataaatac tggctgtgat gacgggtaaa ccgctaacga
    27601 gaaggtgtca ttgccacagg attgaccgcg cagtgcattc gccgccgcga tgacgttttc
    27661 gtagttaccg ccagaacagc ccgcgataat cccctgctgc actttcaggc gaccattttc
    27721 cactttgtcc agcagcgaga gtttggcttt accgtgcgcc acgcgttcgg actcaatttc
    27781 aatctcacgc agaatgtcgg tcaggttctg gttcagtgtg tcgatttcat acacgttgct
    27841 cgggtggaac ggcagcgcaa tcattggttt gatggcgctt aaatcaacgc tgatgcagcc
    27901 atcgtagtac gccatcggtt gagggttaag ctggcagtaa tcctggccgc gaccgtgcag
    27961 cgccagccag ttatggactt cttcatcggt ttgccagaca gaacttaaac aggtcgtttc
    28021 agtggtcata acgtcaacgc tgttacggaa atcggtagag agcgcagcaa caccgggacc
    28081 tacgaattcc atcactttgt ttttgacgta gccgtttttg aacacggcac cgatgatagc
    28141 cagcgccaca tcctgcggcc ccacatacgg cgctggtttt ccggtcagat gcaccgcaac
    28201 aactcccgga tagtcgatat cccaggtgtc attaagcaat tgttttacca actcaccgcc
    28261 gccctcaccg actgccattg tccctaatgc accgtaacgg gtgtggctgt ctgacccgag
    28321 gatcattttg ccgccgcctg ccatcatctc acgcatatat tgatggatga ccgcaatatg
    28381 cggaggcaca aaaataccgc cataacgctg ggccgccgat aaaccaaaaa catggtcatc
    28441 accgttaata gtaccgccga cggcgcagag tgagttatgg cagttggtca gcacatacgg
    28501 cagcgggaaa cgttccattc cggacgcttt agcggtctgt acaataccaa caaaggtaat
    28561 atcgtgagag gctaatgaat caaacttaat tttaagttta tccatatttc cggacgtatt
    28621 atgagaagag agaatagacc aggcaatagt gccttttttt gcttcttctt ttttaatttc
    28681 gccggtgaaa tgttcttcgg caattatttc gttattactg gcgagaaaca cgcctttttc
    28741 agataacttg atcatcaaat gctccagata tctgcgcacc gtgaagtgcg cagaaagata
    28801 ataaatggat atactctaaa taattcgagt tgcaggaagg cgacaagcga gtgaatcccc
    28861 aggagcttac ataagtaagt gactggggtg agcgaacgca gacgcagcac atgcaacttg
    28921 aagtatgacg agtatattta cagcatgttc cagccgatca gcatattcca ccaccacacg
    28981 ccgagggtga tatgcaccag gaaggttaat atcgtcagta ccgcaccgac caaccaccag
    29041 gatttaatat cgttgtaacc cacgccaaag atgaccggac ctgccgcgcc gccatagtga
    29101 gtaaccatgc cgccatagga gttggagaac aacagtgcca gcgcggttaa cattaacggt
    29161 gcgccggaga cgttcgccag catggcaaat acaggtaaca tagcaacgat ataggcacta
    29221 ccggaagcga agaaataacg cacgatgatg ctgaggaaaa taataacgaa gaaagcaacg
    29281 ttaccgtgac catcaaatgc caggttattt ttaaagactt cagctaacca ttcgaagaat
    29341 ttaactttcg ataataagga gcttaagccg ataataccgc cgtaccaaat taaagtattc
    29401 cagccgcctt tatttttaac cacgtcttcc caggtaacaa tacccagcag cagcatggtt
    29461 gccataacaa cgattgccac ggtggattca tcaaccccca gagacttact gaaaatccag
    29521 cccagcagcg ccagcacaaa gacaccgagc agcatttttt cgcggatttt catcggtccc
    29581 agttcggcaa ggcccgcttt agcgatggtt ttgttatcca ccttcttaat ttctggcgga
    29641 tacatggtgt aaatcaccag cggagtaacc agcagcataa tgatgcccgg caatccggcg
    29701 gccagcgccc atccccccca gctaatttgc aggtgcagaa tgtcgttgat cattttcagc
    29761 gccagaatgt tccccgccat tgcggtaaag aacatatagc tggtggtttt ggtgaccatg
    29821 taaatggaca tcatcaggta atgtccgaca cgacgcggac ttttttccgg ttcggacccc
    29881 aaagccaccg ccacgctgtt gatgatcggt aacacaatgc cgcccgcacg cgcggtgtta
    29941 gacggtgttg ccggagccag taccagatcg aggaaaaccg taacgtaacc cagacccagc
    30001 gtggtgttac cgattttacc aatcagcaga taggcaatac gtttacctaa accgatggtc
    30061 acaaatgcgg cgcttaaggt aaacgccgag aacaccagcc aggtagtacc tgaagagtaa
    30121 ccgcttaata cggcggtggt tttaaacgca ccgtcggata agttaccgac caccaccatg
    30181 gaggcagcaa cggcaattaa cagtacgaca ggttccggga aaggcttgat aaccagcccc
    30241 acaatggccg ctaggtaaat accaaaaagc acccacgcca gttcgcttaa tcctgccgga
    30301 gctggcataa agccaataat acatgggatc gctaatatca gaattagctt ccataacgat
    30361 ttcttattca tttaaaatcc tgttctttct gtatgactca tacagaagtc ctttattgat
    30421 tgtccttatc ctgacaacga atcttttcag ggaagataaa cttcaccgga aaatattttt
    30481 ctggtcgtcc gaataacaga tgcgcgtaac gtcgtggcat cctgaccttc attacttaaa
    30541 tgaacgtcga gcgcaccact ggggtgttca atattgatat tgccatatcc tacagaaggg
    30601 atgatttgtc gggtgacggt gccttccaat gcacaactac tggaaatagc aatagcaccg
    30661 gttatcgcca acgcgcgatg gcaagaatgc ggcataaaat aacgcacatt aattgccccg
    30721 cctttctgcg ctggagaaat aagcacaggt ttagggataa ccatattact gacatcacct
    30781 aagcccattg ctttacccgc ttgtagacgg atagattcaa tgcgggctaa taatgctttg
    30841 tcggcatcca gttccgccgg taattcatag cctgtttttc ccagatattc agccggaata
    30901 atgacgactg gcatcgccat atcgatacag gtcaccggga catcgtcaaa ataatcaatc
    30961 tgattatcag tcgggaaaac ttttccggtt ttggttccag cggcattcag gaaagtgagc
    31021 gcaaccggtg cggcagtacc cggtacgccg tcaattctgg cactaccctc gtactcgaca
    31081 acaccatttg gcgtttgcac atcagcttcg atgaacgtac ccgtattgac gttgcggata
    31141 cgtacgcggg taactggcga agtcgctgca atcaaaccat tttcaatggc gaatgcccca
    31201 acgccagaca gcatattgcc gcagttaggc gtggtatcga cacgttgctc atggacgata
    31261 acttgagcaa acagataatc aacatcagca cgcggatcgc tggaacggct aataatggcg
    31321 actttactgg tcagtggatt accgccgcca ataccgtcaa tttccagatc gttaccggaa
    31381 cccataattg ccatcaatat tttatcgcgc tgcgtttgat cttcgggtaa atgttccgct
    31441 aacaggaacg cgcccctcga ggttccacct cgcatcatca cgcagggtat ttttttcata
    31501 ataatgccct caatttaaaa acataactta ttgcgatatg gttacattaa gggcaaagca
    31561 tctctaatga ctttagctca acgattaata acattttttc attaatttaa gttggctaaa
    31621 tgcagttaaa atttttaacg gccagccacc caaaatcgtc gcttccattt cccggagtaa
    31681 taatgaagca tgaattatca agtatgaagg catttgtcat actggcagag tccaattcat
    31741 ttaacaatgc cgctaaatta ctcaatatta cgcaacctgc attaacacga agaataaaaa
    31801 agatggagga agatttacat attcaactat ttgagcgtac aactcgcaag gttacgttaa
    31861 caaaagcagg aaaaaggttg ctcccagagg cgcgggaatt aataaagaaa ttcgatgaaa
    31921 cgctttttaa tattcgtgat atgaatgctt atcatcgtgg tatggtgacg ttagcgtgta
    31981 ttccgaccgc agtattttat tttttaccgc tggcaatcgg taaatttaat gagctgtatc
    32041 ccaatattaa agtgcggatt ctggaacaag gcacgaataa ttgcatggaa tcggtgctat
    32101 gtaacgaatc agactttggc ataaacatga acaacgtcac aaattcatcc attgatttta
    32161 gcccactggt caacgagccg ttcgtgctgg cctgtcggcg tgaccatcca ttggccaaaa
    32221 agcagcttgt agaatggcag gagttagtgg gttacaaaat gattggcgta cgctcctcca
    32281 gcggcaaccg actgttgata gaacaacagc tagccgacaa gccctggaag ctggactggt
    32341 tttacgaagt gcgtcatctt tcgacgtcat taggactggt ggaggcagga ctggggattt
    32401 cagcgcttcc tggtctggca atgccccatg cgccctattc ttcgattatt ggtatcccac
    32461 tggtggaacc cgttatacgt cggacattgg ggattattcg ccgcaaagat gccgtgcttt
    32521 ctccggcagc tgaacgcttt tttgccttgc tgattaattt gtggactgac gataaagaca
    32581 atttgtggac caatatcgtc gaacgccaga gacatgcgtt acaggagata ggttaataca
    32641 ccggcgcagg agagactgcg ccgggtaaat cagcggttag tgtgcgttaa ccaccaccca
    32701 catcggtccc tgcccgaccg catagcggcc tttttcatgc agtagcccct gctcaccaac
    32761 aatttcgtat accgagatat ggtgagattt ttgcccggcg gcaatcagat acttaccacg
    32821 gtgatcgata ttaaagccgc gcggctgggt ttcagttggc tggaagccct ctttactcaa
    32881 cacgctgcca tcttccgaaa cgctgaagac agtaatcagg ctggcggtac ggtcacaggc
    32941 atataagtga cgaccatccg gggtaatatg aatatcagcc gcccaacggg tgtcggagaa
    33001 gttttccggc atcatatcca gcgtctggac acattcgata ttgccgtgcg gatctttcag
    33061 ttcccagaca tccactgagc tgtttaactc attgacgcaa tacgcatatt gttcgtttgg
    33121 atggaatacc atatgacgcg ggccggcccc ttcaacggta gtcacttccg cagggtcctg
    33181 cgccaccaga tgaccatcat cgctgaccgt aaacaggcaa atgcgatcct gctttaatgc
    33241 cggaacccac agcgtacggt tgtccggtga gatattggcg gaatggcaac cgtccagccc
    33301 ctcgaccaca tcgacgacgc caactggcag gccatcttcc agacgcgtta cgctcacgtt
    33361 acccgcattg taagaaccta caaagacaaa ctgcccctgg tgatcggtgg aaatatgcgt
    33421 cggactaccc ggcagcgcag actctgcggc aaaggtcagt gcgccatcgt ccggggcgat
    33481 acgatacgcc aggacgcgaa actcagggcg aacaccaaca tagagataac gtttgtccgg
    33541 gctgaccacc atcggctgca cctgccccgg cacatcgaca acctgtgtca gcgtcagtgc
    33601 gccttcatga ttcaggttcc agacgtgaat ttgctggctc tcagggctgg cgatataaac
    33661 tgtttgcttc atgaatgctc ctttgcattt agcaactgac tgtaacagct aaaattagtc
    33721 gcttttggcg gtgaatgctg aattaaaacg cagaattttg tccgatagtg gtatcggtgt
    33781 accattcgcg cagaagaaaa ttcttaatct taatctggaa tacgccatga ccacacgcgt
    33841 gattgctctc gacttagacg gcaccttatt gaccccgaaa aagaccctgc taccttcatc
    33901 gatagaagcc ctggcccgcg ctcgcgaagc aggttatcaa ttaatcatcg tcacaggtcg
    33961 ccatcacgtc gctattcatc ctttttatca ggcgctggcg ctggatacac ctgctatttg
    34021 ctgtaatggc acctatttgt atgattatca tgcaaaaact gtgcttgaag ccgatccaat
    34081 gcctgttaac aaagccctgc aactcattga gatgctgaat gaacaccaca ttcacggtct
    34141 gatgtatgtc gatgacgcga tggtttatga gcacccgacc gggcatgtca ttcgcacgtc
    34201 taactgggcg caaaccctgc cgccggaaca gcgtccgact ttcacacaag tcgcttctct
    34261 ggctgaaacg gcgcaacaag ttaacgccgt atggaagttc gccctcacgc acgatgacct
    34321 gccgcaattg cagcattttg gtaagcatgt cgaacatgaa ctgggactgg agtgtgaatg
    34381 gtcctggcac gatcaggttg atattgcacg cggcggcaac agcaaaggta aacgtttgac
    34441 gaaatgggtt gaggcgcaag gctggtcgat ggaaaacgtc gtggcgttcg gcgataactt
    34501 taatgatatc agtatgctgg aggccgctgg tacaggcgtg gcgatgggca acgccgatga
    34561 cgcggtaaaa gcgcgcgcca acattgtgat tggtgataac accaccgaca gcattgccca
    34621 gttcatttat agccacctga tttaatcagg cggttatcga cacactttta atttgcgcgt
    34681 acagccacag gccaggtttg atcgccagtt catccctggc ccacgggctg atacgcgccc
    34741 acagcgtttt accgccgacc tccagttcca cttccacctg gccgttgtcg tcataactat
    34801 taacaacttt tgcccgcaat acgttacgaa tgctggtttg ctgcggcggt tgtaaaacca
    34861 gagaaacatc ggaagcctga atgcggatac gtagcgcagc ttgcagcggt tcgtccagct
    34921 tattgaccca caaatgctga tcgcccagcg ccagcgcagt catcgcgtaa tgcggatgat
    34981 gctcaagtac cgtcactttc agaatgctac tttgttgctc tttcggcagc cacggattca
    35041 tcacgctact accccagact tcttccagcg cgccaaaggc tttcacctga ccgttttcca
    35101 gtaccatcac tctgtctgcc agatggagga tctcatccag cgaatggctg acatacaaca
    35161 tcgggatgtt gatttcccgc gtcagacgtt gcagataagg caacagttcg cgtttacgcg
    35221 gaatatccag tgacgccagc ggttcatcca gcaacagcaa ttctggtgcc gtcagcaaag
    35281 cccgcccaat cgctacgcgc tgtttttcgc ctccggacag gctgcctggt aaacggtcaa
    35341 gcaacggttc aatgcctaaa agcgccacca gcttatcgaa ctgatcgacc atgcttttcg
    35401 ccatgccgta gcgcagattg ccacgcactt tgtaatgcgg gaacagccgc gcatcctgaa
    35461 aaacatagcc aacgcgacgc ttttccggcg tcaggcagat acctttctcg gcatcattta
    35521 gtacccgacc attgaggaca atcctccctt tttgcgggcg cgtcagtcca ctgatggcgt
    35581 taatcagcga agtttttccg gcaccggaga cgccaaagat agcagtgatg ccattggcgg
    35641 gcagcgtttc attaatagtc aggcaatggt tgcccaacgt ctgggaaaaa ttcagttcca
    35701 gcatgattag cgccccgccc gttcacggct gattctggcc agccattctg aaatcaacag
    35761 ggagatcatc gccagcgcaa tagaaataat gcacagtctc gctgcgccac tttccccgcc
    35821 gggggtctga atcagggtat acatggcaga aggaatggtt cgcgtttcac caggaatgtt
    35881 cgacacaaag gtgatggttg caccaaactc accgagagaa cgagcaaaag ccagtaccgt
    35941 accaacaata atgcccggta atgtcagcgg taacgtgata gtaaagaaaa cgcgccagcg
    36001 cccggcaccc agtgttcttg cggcctgttc cagtttgaca tcaactcctt ccagcgccag
    36061 acgaattgcc cgcaccatca gcggaaacga catgacggcg gcagcgagaa ccgcgccgcg
    36121 ccagctaaag gcgaaggtaa tgccaaacca gtcatacaaa cgttcaccga taaatccgcg
    36181 ccgtcccatc gaaactaata ataagtaacc gacgaccacg ggcggtaaca ccagcggtag
    36241 atgcagtacg ctgtcgagca gagctttgcc cggaaacgtg caacgcacca gtaaccaggc
    36301 aaaaaagatc ccaaacggca ggctaaacag cacagccagg gaagaaactt tcaggcttaa
    36361 taaaactgcc tgccattctg gatcggtcag tatcattact tggttgtaaa tccgtaacgt
    36421 ttaaagattt ccgcagcctg cggtcccttc agataatcat aaaatgcttt cactgtcgca
    36481 ttgttatgcc cttccacaac agcaaccgga tattccactt ttttatgtga atcttccggg
    36541 aaggtggcaa ccacttttac ccctttgctg gcaactgcgt cagaaccgta aacaatgccc
    36601 aacggcgctt cattgcgttc gaccaacgcc agcgcgccac gaacatcttc cgccggagcc
    36661 agtttcgggg agagcgtatc ccatgcgccc agtttttgca gtgcttcttt tgcataaatg
    36721 ccagcgggaa catgttccgg atcgccaacc gccaggcgac cgccattcag cagtgaagtc
    36781 cagttggttt tgctgtcgat ggtaaaatct ttctgctcgc tggctttcgg tgctacaacg
    36841 accaggctat tgccgagcag tgtctgacgc gtagctgtat cgatcgcttt tttatcaacc
    36901 gcataatcca tccatttctg atcggcagaa ataaacagat ccgcaggggc acccgcttca
    36961 atctgacggg cgagagtaga tgacgaagcg aaagaagaaa ccacatccac gcctttctct
    37021 tttttatact gcgtagcaat gtcctgcatt gcgttagtca gtgatgcggc ggcgaacacc
    37081 gtgattttcc cttcatctgc cagtgcattg ccagcaacag cgaaagagag tgccgcccca
    37141 gcaaacaagt tcaaccattt acgagccatt ggtaacccct taatgtaacg ttatgtaggc
    37201 gacaatataa cgactaactc ggaattttcc cagcagttat tgctaacctt tgtgaggtag
    37261 ataagaaaaa atatcggcag gaaaagcagg aagttgagaa aaagaaaatg cccgactaag
    37321 cgggcattca gggaatcaat gattttgtcc aggctggtct tttttaccaa caccagaaaa
    37381 gatgttgaat acttcaccaa gaccgtaaat cagacccagg atgatggcca tcacgacagg
    37441 taccatgatt acggcgaata ccagactttt taataactct aacatggtca actccagata
    37501 tagtcatgag actattctaa ccgctaagca cagaaaagca ctcccctttt gtgcggtcag
    37561 ctttgcgtgg cgttcgtttt ccgtcacaat aagacttttg ccaggacatt gttatgcagg
    37621 ccgaaatcct tctcaccctt aagctccaac aaaaattatt cgccgacccg cgccgcattt
    37681 cgctactaaa acacattgcg ctttccggtt ccattagcca gggagcgaaa gatgccggta
    37741 tcagctataa aagcgcctgg gatgccatta acgagatgaa tcagttaagt gagcatattc
    37801 tggtcgagcg cgcaacaggc ggtaaaggtg gcggcggcgc agtactgacc cgctatggtc
    37861 agcgactgat tcagctctat gacttactgg cgcaaatcca gcaaaaagcc tttgatgtgt
    37921 taagtgacga tgacgccctg ccgctgaaca gcctgctggc cgcgatctca cgtttttcac
    37981 tgcaaaccag cgcccgtaac cagtggttcg gtaccatcac cgcccgcgat catgatgacg
    38041 ttcaacagca tgttgatgtc ttactggctg atggaaaaac acgcctgaaa gtcgcaatta
    38101 ccgcacaaag cggcgcgcgt ctggggctgg atgaaggcaa agaagtgttg atattgctaa
    38161 aagcgccgtg ggtaggcatt actcaggacg aagcggtcgc gcaaaacgct gacaaccaat
    38221 taccgggtat tattagtcat attgagcgcg gcgcagagca gtgcgaagta ttaatggcgc
    38281 taccggacgg gcaaacactg tgcgccacag tgccggtaaa tgaagcgact tctcttcagc
    38341 aaggacagaa tgtcacggcc tactttaatg ccgacagcgt gattatcgcc acgctgtgct
    38401 aagcgtgttg acaatttgtt atgaaacacg tatccctgtc agtaatcgct gcacaaaatg
    38461 ggatataaaa tgtcatcgtt gcaaattttg caaggcacgt ttcgtcttag cgacacaaaa
    38521 acgctgcaat tgcctcagct aacgttaaac gcgggtgata gttgggcgtt tgtcggttcg
    38581 aatggaagcg ggaaatcggc cctggcccgc gcgctggcgg gggaacttcc gcttttgaaa
    38641 ggtgaacggc aaagccagtt ttcccacatc actcgtctct ccttcgagca attgcaaaag
    38701 ctcgtcagcg acgaatggca gcggaataac accgatatgc tcggccctgg cgaagatgac
    38761 accggacgca ctacggctga gattattcag gatgaagtaa aggatgcacc gcgctgcatg
    38821 caactggcac agcagttcgg tattaccgcc ctcctcgacc gacgctttaa atacctttcc
    38881 actggcgaga cgcgaaaaac cctgctgtgt caggcgctga tgtcggagcc tgacttgttg
    38941 attcttgatg agccgttcga tggcctggat gttgcctcac gtcagcagct ggctgagcga
    39001 ctcgcctcgt tacatcagtc cggtattact ctggtactgg tgctcaatcg cttcgatgag
    39061 atcccggagt ttgtccagtt tgctggcgtg ctggcggatt gcacgttagc ggaaactggc
    39121 gctaaagagg aactgctcca acaagcactc gtcgcgcaac tggcgcatag tgaacagctt
    39181 gaaggtgtgc aactgccgga gccggatgaa ccttcagcac gtcacgcctt acccaccaac
    39241 gaaccgcgca ttgtgctgaa taatggcgtg gtttcttata atgatcgccc cattcttaat
    39301 aaccttagct ggcaggtgaa tcctggcgag cactggcaaa ttgtcgggcc aaatggtgcg
    39361 ggaaaatcga cgttattaag cctgattact ggcgatcatc cgcaaggtta cagcaacgat
    39421 ttgacgcttt tcggacgacg tcgtggcagc ggcgaaacca tctgggatat caaaaagcat
    39481 atcggttacg tcagcagtag tttgcatctg gattaccggg tcagtactac tgtgcgtaat
    39541 gtgattcttt gtggctattt tgattcgatt ggcatttatc aggccgtttc ggatcgccag
    39601 caaaaactgg tgcagcagtg gctggatatt ctcggcattg ataaacgcac ggctgacgct
    39661 ccgttccata gtctttcctg gggacagcag cgtctggcgc tgatcgtccg cgcactggtg
    39721 aaacatccga cgttgcttat tctcgacgaa ccattgcagg ggcttgatcc gctgaatcgc
    39781 cagcttattc gccgttttgt tgatgtgctg attagcgaag gtgaaacgca attgttgttt
    39841 gtttcgcacc atgctgaaga tgcgccagct tgtattaccc atcgtctgga gttcgtgccg
    39901 gacggtggcc tctatcatta tgcgctgaca aaaataaact aagtcggtaa tgctgactgt
    39961 gccggatgcg gcgtaaacgc tttatccggc acacggtcag acgcatccag gcatgaaact
    40021 gcgtcttttt ttcagataaa aagcgcaatc attcacaatc cctctgtttt ataatcactt
    40081 aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat tccactaatt tattccatgt
    40141 cacacttttc gcatctttgt tatgctatgg ttatttcata ccataagcct aatggagcga
    40201 attatgagag ttctggtcac cggtggtagc ggttacattg gaagtcatac ctgtgtgcaa
    40261 ttactgcaaa acggtcatga tgtcatcatt cttgataacc tctgtaacag taagcgcagc
    40321 gtactgcctg ttatcgagcg tttaggcggc aaacatccga tgtttgttga aggcgatatc
    40381 cgtaacgaag cgttgatgac cgagatcctg cacgatcacg ctatcgacac cgtgatccac
    40441 ttcgccgggc tgaaagccgt tggcgaatcg gtacaaaaac cgctggaata ttacgacaac
    40501 aatgtcaacg gtactctgcg cttgattagc gccatgtgcg ccgctaacgt caaaaacttt
    40561 atttttagct cctccgccac cgtttatggc gatcagccca aaattccata cgttgaaagc
    40621 ttcccgaccg gcacaccgca aagcccttac ggcaaaagca agctgatggt ggaacagatc
    40681 ctcaccgatc tgcaaaaagc ccagccggac tggagcattg ccctgctgcg ctacttcaac
    40741 ccggttggcg cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat tccgaataac
    40801 ctgatgccat acatcgccca ggttgctgta ggccgtcgcg actcgctggc gatttttggt
    40861 aacgattatc cgaccgaaga cggtactggc gtacgcgatt acatccacgt aatggatctg
    40921 gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt acacatctac
    40981 aacctcggtg ctggcgtagg cagcagcgtg ctggacgtgg ttaatgcctt cagcaaagcc
    41041 tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg agggcgacct tccggcctac
    41101 tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg cacactcgat
    41161 gaaatggcgc aggacacctg gcactggcag tcacgccatc cacagggata tcccgattaa
    41221 ggaacgacca tgacgcaatt taatcccgtt gatcatccac atcgccgcta caacccgctc
    41281 accgggcaat ggattctggt ttcaccgcac cgcgctaagc gcccctggca gggggcgcag
    41341 gaaacgccag ccaaacaggt gttacctgcg cacgatccag attgcttcct ctgcgcaggt
    41401 aatgtgcggg tgacaggcga taaaaacccc gattacaccg ggacttacgt tttcactaat
    41461 gactttgcgg ccttgatgtc tgacacgcca gatgcgccag aaagcaacga tcctttgatg
    41521 cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct tttcaccgga tcacagtaaa
    41581 acgctgccag agctcagtgt tgcggcattg acggaaatcg tcaaaacctg gcaggagcaa
    41641 accgcagaac tggggaaaac gtacccatgg gtgcaggtct ttgaaaataa aggcgcggcg
    41701 atgggctgct ctaacccgca tccgcacgga caggtttggg caaatagctt cctgcctaac
    41761 gaagctgagc gcgaagaccg cctgcaaaaa gaatattttg ccgagcagaa atcaccaatg
    41821 ctggtggatt atgttcagcg cgagctggca gacggtagcc gtaccgttgt tgaaaccgaa
    41881 cactggttag ccgtagtgcc ttactgggct gcctggccgt tcgaaacgct actgctgccc
    41941 aaagcccacg ttttacggat caccgatttg accgacgccc agcgcagcga tctggcgctg
    42001 gcgttgaaaa agctgaccag tcgttatgac aacctcttcc agtgctcctt cccctactct
    42061 atgggctggc acggcgcgcc atttaatggc gaagagaatc aacactggca gctgcacgcg
    42121 cacttttatc cgcctctgtt gcgctccgcc accgtacgta aatttatggt tggttatgaa
    42181 atgctggcag agacccagcg agacctgacc gcagaacagg cagcagagcg tttgcgcgca
    42241 gtcagcgata tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa agaaaaaacg
    42301 caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca ggcgcctggc
    42361 cgcgtgaatt tgattggtga acacaccgac tacaacgacg gtttcgttct gccctgcgcg
    42421 attgattatc aaaccgtgat cagttgtgcg ccacgcgatg accgtaaagt tcgcgtaatg
    42481 gcagccgatt atgaaaatca gctcgacgag ttttccctcg atgcgcccat tgtcgcgcat
    42541 gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca actgcgtaac
    42601 aacagcttcg gcggcgtgga catggtgatc agcggcaatg tgccgcaggg tgccgggtta
    42661 agttcttccg cttcactgga agtcgcggtc ggaaccgtat tgcagcagct ttatcatctg
    42721 ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca gtttgttggc
    42781 tgtaactgcg ggatcatgga tcagctaatt tccgcactcg gcaagaaaga tcatgccttg
    42841 ctgattgact gtcgctcact ggggaccaaa gcagtttcca tgccgaaagg tgtggctgtc
    42901 gtcatcatca acagtaactt caaacgtacc ctggttggca gcgaatacaa cacccgtcgt
    42961 gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag ctctgcgcga tgtcaccatt
    43021 gaagagttca acgctgttgc acatgagctg gacccaatcg tggcgaaacg cgtgcgtcat
    43081 atcctgactg aaaacgcccg caccgttgaa gctgccagcg cgctggagca aggcgacctg
    43141 aaacgtatgg gcgagttgat ggcggagtct catgcctcta tgcgcgatga tttcgaaatc
    43201 accgtgccgc aaattgacac tctggtagaa atcgtcaaag ctgtgattgg cgacaaaggt
    43261 ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg tcgcgctgat cccggaagag
    43321 ctggtgcctg ccgtacagca agctgtcgct gaacaatatg aagcaaaaac aggtattaaa
    43381 gagacttttt acgtttgtaa accatcacaa ggagcaggac agtgctgaac gaaactcccg
    43441 cactggcacc cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac gcagggatgg
    43501 tagtcacgct gatggactgg ggtgcgactt tactttcagc ccgtattccg ctttccgatg
    43561 gcagcgtccg cgaggcgctg ctcggctgtg ccagcccgga atgctatcag gatcaggccg
    43621 cgtttctggg ggcctctatt ggtcgttatg ccaaccgcat cgccaatagc cgttatacct
    43681 ttgacggtga aaccgtgacg ctttcgccaa gtcagggcgt taaccagctg cacggcggcc
    43741 cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca gaacgatcgt caggtgctgt
    43801 ttgccctgac ttcagatgat ggcgatcagg gcttcccggg taatctcggc gcgacggtgc
    43861 aatatcgtct gaccgacgat aaccgtatct ccattactta tcgcgccaca gttgataaac
    43921 cttgcccggt gaatatgact aatcacgtct atttcaatct tgacggcgag cagtctgacg
    43981 tgcgcaatca caagttgcag attctggcgg acgaatatct gccggttgat gaaggcggca
    44041 ttccgcacga cggcctgaaa tctgtcgccg gaacgtcttt tgatttccgc agcgccaaaa
    44101 tcatcgccag tgagtttctt gccgacgacg atcagcgcaa agtaaaaggt tacgatcacg
    44161 cgttcttgtt acaggccaaa ggcgatggca agaaagtggc ggcgcatctc tggtcagcag
    44221 atgaaaaatt gcagctgaag gtctacacca ctgctccggc tctgcaattt tactccggca
    44281 atttcctcgg cggcacaccg tcgcggggaa ccgaacctta cgccgactgg caagggctgg
    44341 ctctggaaag cgagtttctg ccggacagcc cgaaccaccc tgaatggccg caaccggact
    44401 gcttcctgca tcctggtgaa gagtattcca gcctgacgga atatcagttt attgctgagt
    44461 aagtttaagc cctcgtcatg agggctttat ctcatattgt tcaaatcacc agcaaacacc
    44521 gacatatttg caactcaata ttcacaacaa ccttacactg cgccactatt ttcgctatgg
    44581 ttatgcgtaa gcattgctgt tgcttcgtca cggcaatata atgagaatta ttatcattaa
    44641 aagatgattt gaggagtaag tatatggctg taactaagct ggttctggtt cgtcatggcg
    44701 aaagtcagtg gaacaaagaa aaccgtttca ccggttggta cgacgtggat ctttctgaga
    44761 aaggcgtaag cgaagcaaaa gcagcaggta agctgctgaa agaggaaggt tacagctttg
    44821 actttgctta cacttctgtg ctgaaacgcg ctatccatac cctgtggaat gtgctggacg
    44881 aactggatca ggcatggctg cccgttgaga aatcctggaa actgaacgaa cgtcactacg
    44941 gtgcgttgca gggtctgaac aaagcggaaa ccgccgaaaa gtatggcgac gagcaggtga
    45001 aacaatggcg tcgtggtttt gccgtgactc cgccggaact gactaaagat gatgagcgtt
    45061 atccgggtca cgatccgcgt tacgcgaaac tgagcgagaa agagctgccg ctgactgaaa
    45121 gcctggcgct gaccattgac cgcgtgatcc cttactggaa tgaaactatt ctaccgcgta
    45181 tgaagagcgg tgagcgcgta atcatcgccg cacacggtaa ctccctgcgt gcgctggtga
    45241 aatatcttga taacatgagc gaagaagaga ttcttgagct gaatatcccg actggcgtgc
    45301 cgctggtgta tgagttcgac gagaatttca aaccgctgaa acgctattat ctgggtaatg
    45361 ctgacgagat cgcagcgaaa gcagcggcgg ttgctaacca gggtaaagcg aagtaaacgt
    45421 cattcgttta aaatgagaaa gccgactgca agtgagtcgg cttttttgtt gctaacaatg
    45481 gagcacattg cctgatgcgg cgctgcgcgt cttatcaggc ctacggtgat tcatcggata
    45541 cgccactctg gcggtgcatc cgacaattaa accttacccg cgacgcgctt ttactgcatt
    45601 cgccagttga cgtaacagag catcggtatc ttcccagcca atgcaggcat cagtgatgct
    45661 cttaccgtag gccagcggct ccccgctctc caggctctga ttgccttcaa ccagatgact
    45721 ttccaccatc acgccaataa tggccttttc gccaccggca atctgctggc aaacgtcagc
    45781 acaaacatcc atctgctttt tgaattgttt tgacgagtta gcatggctga aatcgatcat
    45841 cacctgtgct ggcaggcctg ctttgttcag cccttctttc acttcagcaa cgtgcttcgc
    45901 gctgtagtta ggctctttac cgccgcgcag aatgatatgg caatcgccgt taccgctggt
    45961 attcacaatc gccgaatgcc cccatttcgt tacggacagg aagcagtgcg gcgcaccggc
    46021 ggcattaatg gcatcgatag ccactttaat cgtaccgtcg gtgccatttt tgaagccgac
    46081 cggacaagaa agccctgatg ccagttcgcg gtgcacctgc gattcggtgg tacgtgcgcc
    46141 aattgcgccc cagctcatca ggtcagcgag atattgtggg gtgatcatat cgagaaactc
    46201 acccgccgct ggcagaccgc tgtcgttaat atcaagcagc aatttacggg caatacgcaa
    46261 accgtcgttg atctggaagc tattgtccat atgcggatcg ttaatcagcc ctttccagcc
    46321 caccgtggtg cgcggctttt caaaatagac gcgcattacg atttccagct catctttcag
    46381 ctcttcacgc agcgccagca agcgagtggc gtactcttta gccgctacag gatcatgaat
    46441 tgagcacgga ccaatcacca ctaacaggcg atcatcattc cctttcagga ttttatggat
    46501 cgcttttcgg gcatgggcaa ccgtattagc ggcattttca gtagcgggga atttttccag
    46561 caatgcgaca ggaggaagta actctttgat ttctttgatg cgtaaatcgt cgttctgata
    46621 attcatgtct gttccagtgt tgccatactt atcttagtga atgcaatacc tccaatctat
    46681 atcttccgtc agaatgtgta aacggggttt tacactatga acggattaat cctggaatta
    46741 gcaagaaaaa cgccagattg tcgcgaaaaa cgagatctct cctacaattt ctaactgtaa
    46801 ctcctttgtt tgttaattat ttcaagattc tctgctggat ttctttgcct ggctgaaatc
    46861 ttaaaccaat gccttatatt cacctgcaaa tgcactgttg gaagaggtta tccgacataa
    46921 cgaccataac aggagcatcc tatgaaaatg acaaaactgg ccacattatt tctgactgcc
    46981 actctaagcc ttgccagcgg tgccgcactg gctgccgata gcggagcgca aactaataac
    47041 ggccaggcaa acgccgccgc tgatgcgggc caggtagccc ctgacgcccg tgaaaatgtc
    47101 gcgccaaata acgtcgacaa taacggggta aatacgggtt ctggcggcac aatgctgcat
    47161 ccggatggtt cttcaatgaa caatgacggc atgaccaaag atgaagagca caagaacacc
    47221 atgtgcaaag atggtcgctg tccggatatc aataaaaagg tacaaaccgg tgatggcatc
    47281 aataatgatg tcgataccaa aaccgacggt accacgcagt aaccctgcaa aatagccaat
    47341 aatgggagag cggagtctct cccttttaat ttttcccttc taaaagcagt atgatgtcat
    47401 aaagttaagt gagaataatt aagggatgac tcgtcatggc gcactcacac tcacacacgt
    47461 cctcacacct gccagaagat aataatgctc gtcgcttgtt gtatgctttc ggcgtgactg
    47521 ccgggtttat gctggtagaa gtcattggtg gttttctttc tggttctctg gctttgctgg
    47581 ccgatgcggg tcatatgttg accgatactg ccgccctgct tttcgcccta ctcgccgtgc
    47641 aattttcacg tcgtcctccc accattcgcc acactttcgg ttggctaaga ctgaccacgc
    47701 tcgcggcgtt tgtgaatgcc atcgccctcg tggtaattac cattttgatt gtctgggagg
    47761 cgatagaacg cttccgcacg ccgcgtccgg tcgagggcgg catgatgatg gcaattgccg
    47821 tggctgggtt gctggcaaat atactttctt tctggttact tcatcacggc agcgaagaga
    47881 aaaacctcaa cgtacgagca gcggcactgc atgtgctcgg ggacctgctg ggttcggttg
    47941 gggctattat tgccgcgttg attattatct ggaccggctg gacacctgct gaccccattc
    48001 tctcgatact ggtgtcgctt ctggttctgc gtagcgcgtg gcgactgttg aaagatagcg
    48061 tgaatgaatt acttgagggt gcaccggtat cgctggatat cgctgaactg aagcgtcgta
    48121 tgtgccggga aatcccggaa gtccgcaatg tgcaccatgt gcatgtatgg atggtaggcg
    48181 agaagccggt gatgacgttg catgtgcagg tgatcccgcc acacgatcac gatgccttgt
    48241 tggatcagat ccaacactac ctgatggatc actatcagat tgagcacgcc accattcaga
    48301 tggaatatca accttgtcat gggccagact gccacctgaa tgaaggggtg tctggccatt
    48361 cacatcatca ccattaatgg gacagcgcgc gtgagcctct ttcacgtgcg ctgttgatcc
    48421 acatccggct gccgttgagc gcaataaagg tcaggatgat gtactccaga gacatggcgt
    48481 aaacgccctg aagtgcaaaa ataacgacgc taatcacgtt aataatcacc cacaacagcc
    48541 agttttccac atacttacgc gtcatcagaa tcattgccac gatagataac accatcatgc
    48601 atgaatccca gaacgggaaa gcgtccggtt gcagttcagg catcacaacc tgtaatccta
    48661 atgcttgcat gatgatgact gccacgcggg tcaaaaatgc aaacaccgga ttgataaata
    48721 ccgtcatcag accaatagca atgacacaaa ccgccagcca ggttaatgcc ttcggcaatg
    48781 gtaaccagcg aattttcaac tccgcctcgt tctgactggt ttgtcgcgac cacgcatacc
    48841 agccgtaaat attcgcggca aagaaaaaca cctgtaatag caggctggca tacagctgaa
    48901 tctgaaagaa aataatgcca aacaaggtga cgttgatcag gccaaagaag tagttgctga
    48961 tcttctccag actggcaagg ccaatacaca gcaacccggc gatcgtgcct accgcttcga
    49021 tccatgagag atcataaccg cctgccccta ttggtatatg taccaggata ttctgcacac
    49081 taaaaaaatc catctttccc ccaaagcgta acgttacgcc ttattattcg ttatccacgt
    49141 agtgtagccg caaaatccag catacgattg agcggcacca gcgccctctc tcgcagcctc
    49201 tcatcaacat gaacctcgtg attacttcct tcctgttcta atgcctcagc gatagcctga
    49261 aggccattca tggccatcca cggacaatgc gcgcagctgc ggcaggttgc gccctcaccc
    49321 gcggttggtg cttccagtaa ctctttatct ggcaccgcct gctgcatttt gtagaaaatg
    49381 ccccgatcgg ttgccacgat aagccgctga tgtggcaatg tttttgcagc agcgatcagt
    49441 tgactggtgg aaccgaccgc atccgccata tcgacaatag cttgtggtga ttctggatgc
    49501 accagtatgg cagcatccgg gtattcttct tgcaagcggg ttaacgcctg agtcttaaat
    49561 tcatcatgca caatgcaagc accctgccag cataaaatgt ctgcgcccgt ctgtttttgc
    49621 acgtaacgcc ccagatgttt gtcgggtgcc cagatgattt tttcacccaa actatcaaga
    49681 tgatcaataa gttcgacggc aatgcttgaa gttaccaccc aatctgcgcg cgcttttacc
    49741 gcagcagaag tgttggcgta gacgacgaca gtacgatcgg gatgggcatc gcaaaatgcg
    49801 ttaaattctt caacagggca gccgagatcc agtgaacact cagcctgaag tgtcggcatc
    49861 agaattgttt tttccggact gagaattttg gcggtttctc ccataaatct caccccagcg
    49921 actaacaacg tagaagcggg atgctttgca ccgaagcgcg ccatttccaa agaatcagaa
    49981 atacagccac cggtttcttc tgccagttgt tgaatttcgg gatcggtata gtagtgggca
    50041 accatcaccg cattacgttc ttttagcaga cgttttatct tctcgcggta atacgctttt
    50101 tcatcaatgc ttaacggcgt cggcttcggg gggaaaggat aaatcgccgt gtctggatca
    50161 aacatcacgc tcatcttacc atctcgtttt acaggcttaa cgttaaaacc gacattagcg
    50221 tgatattcgc tgttatcggt gtaatttgtt ttatatgcta aacaagatag tcgaatggtg
    50281 gggaaaagtc actcgaatta cgtatgagat gccgaatgcc acactttgcg tcttattcct
    50341 ccgacgtaaa gttgtttacg cagcaagcgc ggagtatgtc atcagggtca agaattgaaa
    50401 ttgagagatg cctgaatagc aaaaaagcgc ctttagggcg cttttttaca ttggtgggtc
    50461 gtgcaggatg actcgcttcg ctcgcccttc gggccgtcgc cgcgagcggc tccgttgttt
    50521 cgctacgctc aactcaaacc tgctgcaggt tcgaatcttt gagtatcgca gatagcaaaa
    50581 aagcgccttt agggcgcttt tttacattgg tgggtcgtgc aggattcgaa cctgcgacca
    50641 attgattaaa agtcaactgc tctaccaact gagctaacga ccccttgcgg gattatgctg
    50701 ctttaatcat tcaggatggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    50761 gtcaactgct ctaccaactg agctaacgac ccatacgggt gttacctgaa agatttttac
    50821 tcggggccaa tctttaagat tggtgggtcg tgcaggatga ctcgcttcgc tcgccctacg
    50881 ggccgttgct ggcgcaacgt tatccttcac gcttaacatc tgagtttgat gttaaaactg
    50941 gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac
    51001 tgagctaacg acccgagtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta
    51061 agggaggtgc tctcccagct gagctaatca cccgatactg cgctggatac tacaaactac
    51121 ttccaccgaa cccaccgtaa ttagtggtgg gtcgtgcagg attcgaacct gcgaccaatt
    51181 gattaaaagt caactgctct accaactgag ctaacgaccc ggtggtgggt gatgacggga
    51241 tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcacccga
    51301 tactacgctg gatactacaa ctaactaggt tagttggtgg gtcgtgcagg attcgaacct
    51361 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc actttttcgt
    51421 tgctttcggt ttgtttgata tcccgtggca acggcggcat atattactga tttcagactt
    51481 gagcgcaaca aaaatttcga tgtagatcac tcaactgctt atgattcgca cgacacgacc
    51541 agaaataatg cgacttctgg tcgtgtgtta tgcattacat cgcgttcaga cgtttttgcg
    51601 cctgtttagc gccatcagta ccagggtatt tactgataac ctgctggtat acggctttcg
    51661 cttttgcggt gtcacctttg tcctgcatga tgacgccgac tttaaacatc gcatctgcag
    51721 cctttggtga cttcggatag tttttcacta ccgaagcaaa atagtacgcc gcatcatctt
    51781 ttttaccctt gttgtagttt aactgaccca gccaataatt ggcgtttggc aggtaagttg
    51841 aatcagggta atttttgatg aaattctgaa atgccaccat tgcgtcatcc tggcgggatt
    51901 tatcctgcac cagcgcaata gctgcattgt aatccgtgtt tgcatcaccg cttttcaccg
    51961 gcgcgccagc attcgcagta ccagcatcag ctgtcggcgt cgttgatgcc gccgcaccgc
    52021 tttgatcacc gctggttgat tgcgccgctg caccaccgct gctgaggctg tcgatctgca
    52081 acaggatctg cttttgccgc tccacgacct gattcagttg atactgattt tcctgaatct
    52141 gaccacgcag ggaatcaata tcggattgat tatcagagag ttgttgctgg agttgggtta
    52201 aaagctggct gtgagcatta gaaatacgct caagttgagt gacgcggtct tcgaccgagc
    52261 ctgagccgac actactgatt ggtgcctgag caaaagcggc ccagggggcc gctataccaa
    52321 ccagtaacga cagactcaat agttgatgtc tgaagttact gctcatgcaa ttctcttagt
    52381 aaaccagtac cgcacgacgg tttttggagt atgccgcttc gtcatgaccc agtactgcag
    52441 gtttttcttt accgtaagaa acgatggaga tctggtctgc agaaacgcct ttaccctgca
    52501 ggtacatctt aacggcgttc gcacgacgtt cacccaggga gatgttgtat tccggagtac
    52561 cacgttcgtc cgcgtgacct tctacggtga ctttgtaaga cgggttgcta cgcaggaagt
    52621 ttgcatgtgc atccagcatt tgagcgaagt cagaacggat atcgtacttg tccagatcga
    52681 agtaaacgat gttgttctgc tgcagctgtt gcatttgcag acgagcctgc tcttcggaag
    52741 acatgttgcc gttgccgccg ttcgcatcca taccagtgcc ggcacccagc atgccttcgc
    52801 tgccgtcatt gctggcgttc ttgttggaag aacatgccgc aattgccata acaggcagag
    52861 caatcatcag ccctttcagc actttgttca gttgcatttc aatgattcct ttactattca
    52921 attaattatt atcacagata cggcgaccag gcagggaatt tgacctgtcc atcagttgcc
    52981 ggaagacgcg ctttgaaacg cccatctgta gaaaccaaat tcagcacgga tcccatcccc
    53041 tgagaagagc tgtagattac catagtgccg ttaggtgcca gacttggcgt ttcatccagg
    53101 aacgtggacg acagaacttg tacgcctccc gttgccagat cttgtttggc aatgtgctgc
    53161 tgcccaccgt tggagctgac cattaccata aatttaccgt cgctgctgac atccgcatcc
    53221 tggttctgcg aaccttccca ggtaatacgt tgtggcgcac cgccgttgat attcacttta
    53281 taaacctgtg gacgaccggc ctggtcagaa gtaaatgcca ggttctggct gtccgggaac
    53341 caggtcggtt cggtattgtt actgcgacca tcagtcacct ggcggatctg accagaagcc
    53401 aaatccatta cgtacaggtt caggctaccg gttttcgaca aggcgaatgc cagtttgctg
    53461 ccgtctggcg agaatgcagg cgcaccgttg tgacgcggga atgaagccac ctgacgtaca
    53521 gcgccgtttg ccagcgtttg gataaccagc gcggaacgac cgctttcgaa ggtcacataa
    53581 gccagtttag aaccgtctgg tgaccacgcc ggggacatca gcggctgtgg tgaacggtga
    53641 acgacaaact ggttgtaacc gtcatagtca gatacgcgca gttcatacgg gaactgaccg
    53701 ccgttggtct gaacaacgta ggcaatacgg gtacggaacg cacctttaat accggtcagt
    53761 ttttcaaaca cttcatcact ggcggtatga ccagcataac gcagccactg cttgttcact
    53821 ttgtacgagt tctgagcaag tacagtaccc ggtgcgccgc cagtgtcaac aagttgataa
    53881 gcaacattgt aagaaccatc cggattcgga gtgacctgac cgacaactac agcgtcaata
    53941 cccagcgcgg accatgcagc tggttgtact tcctgcgcac tacccggctg ctgtggcaga
    54001 cgagcgcgat ctaacggatt aaatttaccg ctgttacgca agtcagcagc aacgataccg
    54061 ccaatatctt caggtgccgc accaggaccc gcccactgga aaggaacaac accaatagga
    54121 cgaccggaat ctacaccgct gtcgatcaca atgcggactt cagcatgcag aactgatgcc
    54181 cacagtatga gaaaaccaaa tgctactcgt aatgcctgct tcatcatatc tcccttatct
    54241 ggaccgatgg cccacgataa tttagcagaa tgttaacaaa ctaaaataca caaaactacc
    54301 agaactccgt ggcaaccggt gcctgatgtt gaccgtcgga acagtcaaca tcgcgattac
    54361 ggtttgaagt ccaatggcgc gtttttgaac acttcatata ctgcctggct tggtggtttc
    54421 gggatcttcg caagtttagc tgctgccaac gcagcctgac aaagtgcggg atcgccacct
    54481 tcaggtttga tatccagtaa catgccatcg ggtgccagtt ttatgcgcag cgtacaggtt
    54541 ttgcctgcat aggacgatgc gtcatagaac ttactttcga tagcagattt aatctgcccg
    54601 gcatagttat tgatatcggc ccctgatgcg ccattgtttt tagtattacc actcccggca
    54661 ggcgaagcat tgttcccttt cgcccctccc cccgttttcg gtgcattctt accagagctt
    54721 agctcaccga aaatatcatc tgcctctgcg gcagcttttg ctgcagcggc tttttcagct
    54781 gctgcttttg cagctgccgc ttttttgtct gcggcggctt tttctgccgc tgctttctta
    54841 tcagctgctg ccttttcagc agccgctttt tctgctgctt ttttgtcggc tgcagctttc
    54901 tctgctgccg ctttcttatc agctgcagcc ttttcagcag ccgctttctt ctcagcttct
    54961 gccttggctt tttcagcagc ttcagttgcc gctttctttc ttgcttcagc tgcagctgct
    55021 tctgccgctt ccgctttctt cttcagtgcc gcagcggctg cctcggcttt tttctgcgct
    55081 tcggctgcgg ctttggcggc ttctgcttct gcttttttct tcgcgtctgc agccgctttc
    55141 ttcgctgctt cttccgcagc tttagcatcc gcttcggcct tcgctttagc atctgccgct
    55201 gctttcgctg ccgcctcttc cgcttgcttc tgctttaact cggcctgttt tgcggcttct
    55261 tcagcctgct ttttctgttc ctgagcagct aaccgctctt tctcaagttg cttcaggcgt
    55321 tcctgttcag ccgcttgttt ctcacgcagt tcttcagcag cctgctgttc cttcatcttg
    55381 cgctgctcat cagaacgctt cgcgcttgat tcctggcttt gcatgcgttt gtactgctca
    55441 actaccgcac ctgaatcaac catgacagcg tcgatggacg aaccaccgcc gcctccggct
    55501 gaagcttcta tattctcatc gaacgaactc cagatcagcg ccgcaaataa gatgacatgc
    55561 agcactgctg aaataattat cgcccgcttg agcttgtcgt tttgttcggt tgcctttgac
    55621 actctcggtt tccaaaaact gttcgcctgt tacccgctct ctttcaagca agggaaacgc
    55681 agatgtttag ataggctgcg tcattaaacc aaccgatttc acacccgcac tatgtaacaa
    55741 gttcagtgct ttaattattt catcgtaagg cacatctttt gcgccaccga tcagaaagac
    55801 cgttttcggg ttggccttga aacggctgga cacttccgcc accacctgct ctggtggtaa
    55861 acgctcaagg cgatctttct caaccaccac ggtgtactga ccaataccag acacttcaac
    55921 aatcactggc ggattatcgt tactgctcac cgcctgtgat tcagtagcgt ctggcagatc
    55981 gacctccacg ctctgggtga tgatgggcgc tgtcgccata aagatcagca acagcaccag
    56041 cagtacgtcc agcaacggta caatgttgat ttcggacttg agatcgcgac gacctcgtcc
    56101 acgcgctctg gccatggctt accccttgtt gctctcgcta acggtaaacg cctggcggtg
    56161 cagaatcgcg gtaaactctt ccataaagtt gtcgtaattc agttccagtt tgtttacgcg
    56221 ctggttgaga cggttataag ccattaccgc cggaatagcg gcgaacagac cgattgctgt
    56281 agcaatcaac gcttctgcga tgcctggcgc aaccatttgc agtgttgctt gttttaccgc
    56341 cccgagggcg ataaaggcgt gcatgatccc ccagaccgta ccaaacagac caatatacgg
    56401 gctgatggag ccaaccgtac cgaggaacgg aatgtgcgtt tccagatttt caagttcacg
    56461 gttcatggaa atacgcatcg cacgcgacgc cccttccact acggcttccg gcgcatggct
    56521 attggcacga tgcaggcgca caaattcttt gaacccgctg taaaagattt gttccgaacc
    56581 ggtcagatta tcccgtttcc cctggctctc ttgatagagg cgagagagtt cgattccaga
    56641 ccagaattta tcctcaaacg cttcggcttc gcgcgccgct gcgttaagaa tacgggtccg
    56701 ctggataata atggcccagg atgcgattga aaaaccaatc aaaatcaaca tgataagttt
    56761 aaccagaagg ctagccttca ggaacaaatc aaggatattc atgtcagtca ctgcttaaac
    56821 tccgcgacaa tagacttggg aagcgcacga ggcttcattt tgagtgggtc aacgcaaaca
    56881 accagaacct ctgcttcatt cagcaaagta ttctcggcgt tgacaatacg ttgcgtgaaa
    56941 accaaagagg tgccacgcat tgatgttatt tcagtctgta tttcgagcat atcgtcgagc
    57001 cgcgcaggtg cgtaatattc caccgtcatt ttacgtacca caaaggcaac gcgttcagcc
    57061 atcagcgcct gctgactgaa gtgatgatga cgcagcatct ctgtgcgtgc tctttcataa
    57121 aaagcgacgt aactggcgtg gtacaccaca ccgccggcat cggtatcttc atagtagacg
    57181 cgaaccggcc atcgaaacag cgttgtattc actttacatc ccggtaatgc aacaaaagtt
    57241 agagctttta aacgctgcta ctatacgcgc gcgattgagg tttgggaatg agggcaagtt
    57301 aagggagcgt aaaaatttat gggcctggcg cggcccataa ttttttaaag agaattctga
    57361 tttaaaagaa gaagaaaatc agcccgacaa tgagaacaat atcggcaagc aatgggcaaa
    57421 aaatcccttg ccaaagaacc ttctgcggac gaaagcccac gccgtgaatc acaccagcac
    57481 agacggccca catcagcaat aaaccatgcc agatttccag atcactggtc ttcgcggcga
    57541 aacgtgatgg gtcccaaaac atacatcctg ctaacagaag cgccatcacg aaggaaagcg
    57601 cccgcagggg gcgcttgtcc attaccgcat acaaagtcgc gataatctta ctcatcagat
    57661 gtcttcttga ccggctttgc ctgattcgac gtgctcaagc gccagcgcgg tgattacccc
    57721 aaacgaacag gcaagaagcg ttcccagaat ccatgcgaaa taccacattt ttagctcctt
    57781 acttagtaca gagagtgggt gttacgttca atatcttctt tggtgatacg accgaacatt
    57841 ttccagtaac accaggcggt gtagagcaga atgatcggta ccagaaccac cgcaacccag
    57901 gtcatgacgt taagcgtcag ctggctggaa gttgcatccc acattgtcag gcttgcgttc
    57961 atcatggtgc tggacggcat cacaaacggg aacattgcaa taccagcagt caggatgatg
    58021 caggccagcg tcagggagga gaacacaaac gcccatgcgg ctttatccat acgtgcagtc
    58081 aggatggtca gcagcggcag aaccacaccc agtgccggaa tagcccacag aattggcgtg
    58141 ttgttgaagt taaccagcca tgcgccagct tcacgaacca cttctttatt cagcgggtta
    58201 gaggctgcgt aatggtccat tgtcgatttc acgacataac catcgatacc gtacatcacc
    58261 catacgccag ccagtgcgaa acagaccagt gtcaccagtg ccgccacctg agccgttgca
    58321 cgggtacgca ggtgcagttc gcccacggta cgcatttgca gataggttgc gccctgagta
    58381 atgatcatcc ctacgctcac cacgcctgcc agcaggccga acgggttaag cagctggaag
    58441 aagttaccgg tgtagtacag acgcagatat tcatcaacgt tgaacggtac gccctgcaac
    58501 aggttaccga acgctacacc aatcaccaac ggcggaacga agctaccaat gaagatgccc
    58561 cagtcccaca tgttacgcca gcgggtttct tcaatcttgg agcggtagtc aaaaccgacc
    58621 ggacggaaga acaaagacgc cagcacgagg atcatcgcca catagaagcc ggaaaacgca
    58681 gcggcataga ccatcggcca ggcagcaaac agtgcgccgc ctgcggtgat cagccaaacc
    58741 tggttaccgt cccagtgtgg tgcgatggag ttaatcataa ttcgacgctc ggtgtcgtta
    58801 cgaccgagga aacgggtgag catgcccacc cccatgtcga aaccatcagt gactgcaaaa
    58861 ccaatcagca gaacgccaac cagcagccac cagataaaac gcaatacttc ataatcgatc
    58921 atttgacgac tcctgtctta gcgtgccggc tgagtagtcg tggaagactg ctcaaagtga
    58981 tagcgaccgg ttttcaggct gcttgggccg aggcgtgcaa acttgaacat taagaacaat
    59041 tctgccacca ggaacagggt atacaggccg caaatcagca ccattgagaa gatgagatcg
    59101 cctgcggtca gtgacgagtt cgccacagct gtcggcagca cttcaccgat agcccacggt
    59161 tggcggccat attcagccac gaaccagccc gcttctacag caatccacgg cagcggaata
    59221 ccgtacagcg cggcgcgcag cagccatttt ttctcgccaa tgcggttgcg gataacactc
    59281 cagaaagaga gcgcgatgat tgccagcagc aggaagccac acgccaccat gatacggaac
    59341 gcgaagtaca gcggcgctac acgcggaatg gagtctttgg ttgcctgttg aatctgagct
    59401 tcagtcgcat cagccacgtt tggcgtatag cgtttcagca gcagaccata accgaggtct
    59461 ttcttcatgc tattgaactg gtcacgaacc gcctggtcgg tagaaccaga acgcagctgt
    59521 tcgagcagag agtacgcctt catcccgtta cgaatgcgtt cttcatgctg caccatcagt
    59581 tctttcaggc cgataactgg agtatccacg gaacgcgttg caatgatgcc cagcgcgtaa
    59641 gggatctgga tcgcaaattt gttcgtctcc tcttcctgat caggaatgcc gaacagagta
    59701 aaggcagcag gcgcaggttg cgtttcccac tcggcttcaa tagcagccag tttggttttc
    59761 tgcacgtcgc ccatttcgta gccggattca tcacccagaa caataacaga cagaacggca
    59821 gccataccga agctggcagc gatagcaaag gagcgtttag cgaaggcgaa gtcacgacct
    59881 ttcagcatat accatgcgct gataccgagg atgaacatcg cgccagtaac ataaccagac
    59941 gctacagtgt gaacgaattt cacctgagca accgggttaa gcaccagctc ggagaagctc
    60001 accatctcca tacgcatagt ttcaaagttg aaatcggacg cgattgggtt ttgcatccag
    60061 ccgtttgcaa ccagaatcca cagtgcggac aggtttgaac cgagcgccac cagccaggtg
    60121 acacacatat gctgaacttt acccagacga tcccaaccga agaagaacag acctacaaag
    60181 gtggattcga ggaagaaggc catcagacct tcgattgcca gcggcgcacc gaagatatcc
    60241 cctacatagt gggaatagta agaccagtta gtcccgaact ggaactccat ggtcagaccg
    60301 gtagccacac ccagagcgaa gttgatacca aacaacttgc cccagaactt ggtcatatct
    60361 ttataaatct gtttgccgga aaggacgtag accgtttcca taatggccag caggaacgcc
    60421 ataccgagcg tcagtggcac aaaaaggaag tggtacatcg cggtcaaggc aaactgtaag
    60481 cgcgacagtt cgactatatc taacatcatg actccttgct catcgcatga agactccgag
    60541 agtgaacccc gttagaaagg gtcacacgca tgccccaata caaaattatt tgctcgcctc
    60601 tatcgttgcc atttcatcta aagaggaaaa gacaatgata aaaaagttac aaatatgtta
    60661 atgaataaac caaattga
//
LOCUS       DAZGBC010000010        57528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGBC010000010 DAZGBC010000000
VERSION     DAZGBC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.010"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(133..294)
                     /gene="rhuM"
                     /locus_tag="ACHK1J_000644"
                     /pseudo
     CDS             complement(133..294)
                     /gene="rhuM"
                     /locus_tag="ACHK1J_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(373..467)
                     /locus_tag="ACHK1J_000645"
     tRNA            complement(373..467)
                     /locus_tag="ACHK1J_000645"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(431..433),aa:Sec,seq:tca)
     gene            760..2142
                     /gene="yicJ"
                     /locus_tag="ACHK1J_000646"
     CDS             760..2142
                     /gene="yicJ"
                     /locus_tag="ACHK1J_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO7909974.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISIAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIVAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2152..4470
                     /gene="yicI"
                     /locus_tag="ACHK1J_000647"
     CDS             2152..4470
                     /gene="yicI"
                     /locus_tag="ACHK1J_000647"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO7909975.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANAQGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEVPAADGSVIFTLKATRTGNTITVTGAGEVRNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            4765..6318
                     /locus_tag="ACHK1J_000648"
     CDS             4765..6318
                     /locus_tag="ACHK1J_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO7909976.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVKIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            6345..6815
                     /locus_tag="ACHK1J_000649"
     CDS             6345..6815
                     /locus_tag="ACHK1J_000649"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO7909977.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            6833..7147
                     /locus_tag="ACHK1J_000650"
     CDS             6833..7147
                     /locus_tag="ACHK1J_000650"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO7909978.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            7172..8263
                     /locus_tag="ACHK1J_000651"
     CDS             7172..8263
                     /locus_tag="ACHK1J_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO7909979.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            8275..9126
                     /locus_tag="ACHK1J_000652"
     CDS             8275..9126
                     /locus_tag="ACHK1J_000652"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO7909980.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAYAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADMMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            9207..10067
                     /locus_tag="ACHK1J_000653"
     CDS             9207..10067
                     /locus_tag="ACHK1J_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO7909981.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMNILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            10115..11047
                     /locus_tag="ACHK1J_000654"
     CDS             10115..11047
                     /locus_tag="ACHK1J_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7909982.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            11028..11708
                     /locus_tag="ACHK1J_000655"
     CDS             11028..11708
                     /locus_tag="ACHK1J_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO7909983.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEASLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(11752..13461)
                     /gene="yicH"
                     /locus_tag="ACHK1J_000656"
     CDS             complement(11752..13461)
                     /gene="yicH"
                     /locus_tag="ACHK1J_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7909984.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PAQ"
     gene            complement(13582..14973)
                     /gene="xanP"
                     /locus_tag="ACHK1J_000657"
     CDS             complement(13582..14973)
                     /gene="xanP"
                     /locus_tag="ACHK1J_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO7909985.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            15252..16457
                     /gene="gltS"
                     /locus_tag="ACHK1J_000658"
     CDS             15252..16457
                     /gene="gltS"
                     /locus_tag="ACHK1J_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO7909986.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(16633..18714)
                     /gene="recG"
                     /locus_tag="ACHK1J_000659"
     CDS             complement(16633..18714)
                     /gene="recG"
                     /locus_tag="ACHK1J_000659"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO7909987.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALNVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18720..19409)
                     /gene="trmH"
                     /locus_tag="ACHK1J_000660"
     CDS             complement(18720..19409)
                     /gene="trmH"
                     /locus_tag="ACHK1J_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO7909988.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(19416..21524)
                     /gene="spoT"
                     /locus_tag="ACHK1J_000661"
     CDS             complement(19416..21524)
                     /gene="spoT"
                     /locus_tag="ACHK1J_000661"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO7909989.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(21543..21818)
                     /gene="rpoZ"
                     /locus_tag="ACHK1J_000662"
     CDS             complement(21543..21818)
                     /gene="rpoZ"
                     /locus_tag="ACHK1J_000662"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO7909990.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(21873..22496)
                     /gene="gmk"
                     /locus_tag="ACHK1J_000663"
     CDS             complement(21873..22496)
                     /gene="gmk"
                     /locus_tag="ACHK1J_000663"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO7909991.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            22754..24436
                     /gene="ligB"
                     /locus_tag="ACHK1J_000664"
     CDS             22754..24436
                     /gene="ligB"
                     /locus_tag="ACHK1J_000664"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO7909992.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVADVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNQARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLVSTEQFWQQLPGIGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(24433..25050)
                     /gene="yicG"
                     /locus_tag="ACHK1J_000665"
     CDS             complement(24433..25050)
                     /gene="yicG"
                     /locus_tag="ACHK1J_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO7909993.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(25342..26166)
                     /gene="dinD"
                     /locus_tag="ACHK1J_000666"
     CDS             complement(25342..26166)
                     /gene="dinD"
                     /locus_tag="ACHK1J_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO7909994.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQISIKQLENCVKITEKK"
     gene            26891..27265
                     /locus_tag="ACHK1J_000667"
     CDS             26891..27265
                     /locus_tag="ACHK1J_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7909995.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDAAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            complement(27950..28342)
                     /locus_tag="ACHK1J_000668"
     CDS             complement(27950..28342)
                     /locus_tag="ACHK1J_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7909996.1"
                     /translation="MRDIQQVLERWGAWAANNHENVTWSSVAAGFKGMIPPKITSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGKTFMALARKHGCSDGHIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(28825..29014)
                     /locus_tag="ACHK1J_000669"
                     /pseudo
     CDS             complement(28825..29014)
                     /locus_tag="ACHK1J_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999882.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29724..30800)
                     /locus_tag="ACHK1J_000670"
     CDS             complement(29724..30800)
                     /locus_tag="ACHK1J_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7909997.1"
                     /translation="MNLTAMNLVNAIANLPKNRQFDYVNESNAGKIVIDSIKLPEGPI
                     RFKRFNPTKGETLRDAKVDSISSQMLWRLANAIQEDAPINLDRVFGASYNSRSVIESL
                     LAHTPQFYWCKLDRLEVINTKQTIKKGHKHLIYLPNLPHENGKLSEYKTNIVISEMNT
                     EVVYQSVDSETIRPIEGMSIEESRRHAQIQIALVKIGHHLGYRTWVAANDRGLKYNGK
                     AIAQMDGVIDSLSNEKVLSSYSEAIAGARLIDCIWFRNGKLMPAVMEIEHSTGIIRGL
                     ARMKNFCDYAPRLQDIRWTIVAPDEYREKVIEFANRPQFRELDTKFFPYSAVEELYSL
                     CERRKPQGITDQFLDSFMEKCIKH"
     gene            complement(30736..31875)
                     /locus_tag="ACHK1J_000671"
     CDS             complement(30736..31875)
                     /locus_tag="ACHK1J_000671"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO7909998.1"
                     /translation="MSTLKPAIFSFFSGSGFLDLGFENSGYEVVFVNEFHPPFMDAYK
                     HSRAVMNKPIPRFGYAEESIEEVDHNKLKDNIKALKKEKRLIGFIGGPPCPDFSVAGK
                     NKGRDGENGKLSQSYINIIVEMKPDFFLFENVKGLYRTLKHREFFEHLKKQLMDVGYI
                     LHERLINSIEYGVPQDRERIILLGFQNKSLTKIFNWSGRLYPDSSAFEHNWPTTNEFV
                     ENSTLAMPESLPEELTVDYWFRKNKVSEHVNAKHVFTARAGLVRFKAVAEGDDSKKSY
                     KRLHRWRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKEFELPSYMTLSNMFKT
                     VGNGVPYLAALGIAKSILEHLGIKDEFNSNESCECDSKSTQKQAV"
     gene            complement(32922..33083)
                     /locus_tag="ACHK1J_000672"
     CDS             complement(32922..33083)
                     /locus_tag="ACHK1J_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7909999.1"
                     /translation="MTDRSPTEVIHGIIEEQEERDLILMIEEQRERQRRQEASQPDSV
                     SAENQADVP"
     gene            complement(33617..33715)
                     /locus_tag="ACHK1J_000673"
                     /pseudo
     CDS             complement(33617..33715)
                     /locus_tag="ACHK1J_000673"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
     gene            complement(33786..34088)
                     /locus_tag="ACHK1J_000674"
     CDS             complement(33786..34088)
                     /locus_tag="ACHK1J_000674"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7910000.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKTDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            34173..34559
                     /locus_tag="ACHK1J_000675"
     CDS             34173..34559
                     /locus_tag="ACHK1J_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910001.1"
                     /translation="MRLTQYKKEILSYFTPDNLSWVRGEIGNPPFDVSGVAYLLYGID
                     SHNNRYQLESTRRTLEAMVRDGLLERINVYERRDSVGHGVRRKVARYGLPGRSVVARD
                     GEGRNGAIDGECIRINATDVVLVEGQ"
     gene            complement(35010..35303)
                     /locus_tag="ACHK1J_000676"
     CDS             complement(35010..35303)
                     /locus_tag="ACHK1J_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7910002.1"
                     /translation="MIADSVAQKLEERGLWRRAATRWSDVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFADVKKAADRTLKEMGINPQDEWKNYSFSDAGDDLALS"
     gene            complement(35323..35607)
                     /locus_tag="ACHK1J_000677"
     CDS             complement(35323..35607)
                     /locus_tag="ACHK1J_000677"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7910003.1"
                     /translation="MFGAENIKPLKAGVIHDMLQDAKARELAIGHGVLRGALTSYIHT
                     TRYLKAIVAGGARYDLNGQPCGEVTEEDKSVASELLKRRLEQMKQKNSQQ"
     gene            complement(35756..36205)
                     /locus_tag="ACHK1J_000678"
     CDS             complement(35756..36205)
                     /locus_tag="ACHK1J_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7910004.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMTLTRMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGTRGNQPPAGGDDPFSDDIPF"
     gene            complement(36202..36651)
                     /locus_tag="ACHK1J_000679"
     CDS             complement(36202..36651)
                     /locus_tag="ACHK1J_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910005.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERTGKIYEKYAANS
                     KRAARAITYHFMTLYDLKADDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            complement(36930..38753)
                     /locus_tag="ACHK1J_000680"
     CDS             complement(36930..38753)
                     /locus_tag="ACHK1J_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO7910006.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKERGKGDDATSLALNQMGAS
                     QRGEVLLAHYGGELVINADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDVCYSQNA
                     IKSAVDTMKLSLSVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPA
                     EGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGK
                     SVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAIT
                     GGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEIVPENER
                     DPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGY
                     LMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMAE
                     YGREYMKKRTKEGVRSNVILTDESEDWLPSCAPVKNEGDKN"
     gene            complement(38759..38872)
                     /locus_tag="ACHK1J_000681"
                     /pseudo
     CDS             complement(38759..38872)
                     /locus_tag="ACHK1J_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(38939..39133)
                     /locus_tag="ACHK1J_000682"
     CDS             complement(38939..39133)
                     /locus_tag="ACHK1J_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910007.1"
                     /translation="MSDLFDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            complement(39137..39322)
                     /locus_tag="ACHK1J_000683"
     CDS             complement(39137..39322)
                     /locus_tag="ACHK1J_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910008.1"
                     /translation="MLKTFRVFSRAVNARGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDKE"
     gene            complement(39315..39503)
                     /locus_tag="ACHK1J_000684"
     CDS             complement(39315..39503)
                     /locus_tag="ACHK1J_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO7910009.1"
                     /translation="MATIPALVHSQTAFIWRFIIFGASESQIINVTAWTEREARNRCP
                     SGCVAVFAARIRQEVTHA"
     gene            complement(39534..41369)
                     /locus_tag="ACHK1J_000685"
                     /pseudo
     CDS             complement(39534..41369)
                     /locus_tag="ACHK1J_000685"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            complement(41356..42156)
                     /locus_tag="ACHK1J_000686"
     CDS             complement(41356..42156)
                     /locus_tag="ACHK1J_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO7910010.1"
                     /translation="MKRPNMPVQGQGFVQPEELSQQYFAAIIPVSPGIIGGYETGIVS
                     ARALHKALGVGRDFTNWIKGRIEEYEFKKGMNFEVVENLSSPNPASAKSRQQLAHDYL
                     LTVNMGKELAMVERTEQGRAIRQYFIKCEEELHKAAPERAAALRRELKARVTVASFFK
                     PMCAALELYRVELGKETHQHHYTTESNMLARIVLGGMTAKQWAQANGIKGKPRDSMSV
                     MQLEHLSYLEQSNITLIELGMTYQQRKAELIRLSQRWLARRMGEHEHA"
     gene            complement(42224..42439)
                     /locus_tag="ACHK1J_000687"
     CDS             complement(42224..42439)
                     /locus_tag="ACHK1J_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7910011.1"
                     /translation="MTANHQEAIPTTGYIRRCNLAKLLDTSVSTIDRWVRIGKLPKPT
                     KLGEKNTVFNAVEINHWLNERRNNTKQ"
     gene            complement(42826..43476)
                     /locus_tag="ACHK1J_000688"
     CDS             complement(42826..43476)
                     /locus_tag="ACHK1J_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910012.1"
                     /translation="MINILKKSPIERLCASVSITPHEMALALADLNPSMRIGDVPQDK
                     FEQVESARTHIARAIRLHSGVKTSKDEPYRAGDIFLASFPFMEAGTPEAIIKAVTDTI
                     DDLRGTKNWEEKALNLGGRDLVRYIKETSRSGRGQHRKLDEEQGNMKMMGLLVLLLVK
                     KSGTTAYIQDGEPNRSAIYRDVEALMKEKGISPKGIAKSTFMQKISAALLAVSQAD"
     gene            complement(43473..44735)
                     /locus_tag="ACHK1J_000689"
     CDS             complement(43473..44735)
                     /locus_tag="ACHK1J_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7910013.1"
                     /translation="MPKLTDIQIRAWIKADERFDGKADGNGLYLCYPKNYTMPFWRFR
                     YKFAGKQRAMVIGSYAELSLSRARETAKELSARVALGYDVAAEKQERKAEALAKMEAE
                     KNAIRVSELAAEYFERQILPRWKHPDILRRRIDKDINPRIGHMKVEDVKPRHVDEMLK
                     AIVDRGAPTIATDVLRWTRRIFDYGVKRHVLEINPCSAFEVSDAGGKESSRDRWLTRE
                     ELIRFFQAMRTAKGFSRENELTFKLLLALCVRKMELCAARWEEFDLDNAVWHLPEERS
                     KNGDPIDIPLVHPVIEWLRELRVLSCNSAWVLPARKMQHRMIPHIQESTLPVALAKVR
                     AEMPEVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRYQYFNERRD
                     ALAKWTNLLVSLERGEHYNVTPIRRAKS"
     gene            complement(44927..45790)
                     /gene="yicC"
                     /locus_tag="ACHK1J_000690"
     CDS             complement(44927..45790)
                     /gene="yicC"
                     /locus_tag="ACHK1J_000690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO7910014.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            45917..46633
                     /gene="rph"
                     /locus_tag="ACHK1J_000691"
     CDS             45917..46633
                     /gene="rph"
                     /locus_tag="ACHK1J_000691"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO7910015.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            46699..47340
                     /gene="pyrE"
                     /locus_tag="ACHK1J_000692"
     CDS             46699..47340
                     /gene="pyrE"
                     /locus_tag="ACHK1J_000692"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO7910016.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(47377..47973)
                     /gene="slmA"
                     /locus_tag="ACHK1J_000693"
     CDS             complement(47377..47973)
                     /gene="slmA"
                     /locus_tag="ACHK1J_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO7910017.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(48080..48538)
                     /gene="dut"
                     /locus_tag="ACHK1J_000694"
     CDS             complement(48080..48538)
                     /gene="dut"
                     /locus_tag="ACHK1J_000694"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO7910018.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(48516..49736)
                     /gene="coaBC"
                     /locus_tag="ACHK1J_000695"
     CDS             complement(48516..49736)
                     /gene="coaBC"
                     /locus_tag="ACHK1J_000695"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO7910019.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            49908..50576
                     /gene="radC"
                     /locus_tag="ACHK1J_000696"
     CDS             49908..50576
                     /gene="radC"
                     /locus_tag="ACHK1J_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7910020.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            50793..51029
                     /gene="rpmB"
                     /locus_tag="ACHK1J_000697"
     CDS             50793..51029
                     /gene="rpmB"
                     /locus_tag="ACHK1J_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO7910021.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            51050..51217
                     /gene="rpmG"
                     /locus_tag="ACHK1J_000698"
     CDS             51050..51217
                     /gene="rpmG"
                     /locus_tag="ACHK1J_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO7910022.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            51315..52124
                     /gene="mutM"
                     /locus_tag="ACHK1J_000699"
     CDS             51315..52124
                     /gene="mutM"
                     /locus_tag="ACHK1J_000699"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO7910023.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(52163..52642)
                     /gene="coaD"
                     /locus_tag="ACHK1J_000700"
     CDS             complement(52163..52642)
                     /gene="coaD"
                     /locus_tag="ACHK1J_000700"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO7910024.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(52650..53927)
                     /gene="waaA"
                     /locus_tag="ACHK1J_000701"
     CDS             complement(52650..53927)
                     /gene="waaA"
                     /locus_tag="ACHK1J_000701"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO7910025.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSSSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            54340..55398
                     /gene="rfaQ"
                     /locus_tag="ACHK1J_000702"
     CDS             54340..55398
                     /gene="rfaQ"
                     /locus_tag="ACHK1J_000702"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO7910026.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTSLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            55395..56519
                     /gene="waaG"
                     /locus_tag="ACHK1J_000703"
     CDS             55395..56519
                     /gene="waaG"
                     /locus_tag="ACHK1J_000703"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7910027.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            56512..57309
                     /gene="rfaP"
                     /locus_tag="ACHK1J_000704"
     CDS             56512..57309
                     /gene="rfaP"
                     /locus_tag="ACHK1J_000704"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO7910028.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPSDVRVKRMLIKRVATMVRNMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT        13726 a        14065 c        14592 g        15145 t
ORIGIN      
        1 atcttgatgg cagtctgatt attggtgaag tttataggtc agtttttttg ccagctgtta
       61 aaggaataga acagtcgtat aaaaaaaggg tgttctggag cgtgtaaaac aacatcttca
      121 ccttaatggc gatacagttg gctgagagtc agcagggggg aaaatcacct gtaaataaac
      181 acggcgcatg ctggcgcgaa tatcgcggat gcgttcgagg acagaagaac cgacaggcgg
      241 attcttcagc tgctcatcgt ccatcacaaa acctttgacc aggtattcct gaagccgatt
      301 gaatattgag cagtataaaa tatctggatt gacagtatta cgaggggttg gagaacgtat
      361 gaagaggcat tttggcggaa gatcacagga gtcgaacctg cccgggaccg ctggcggccc
      421 caactggatt tgaagtccag ccgcctcacc ggagacgacg atcttccgcg cctcgattgc
      481 tacatggagg cggggcgcat tatagctact tccttgagtt tctacatccc ccagatcagt
      541 tttttccgcc taatacacgc agagtattat cacgaaagca ttatagttca tataaaaccc
      601 atattttaca atgatatatg agcggaatta acgctaccac gatcacataa aacaagaatc
      661 ataaattaat aaccagatat cggaatattc gctctcacag ggatggttta caaaatgcgt
      721 tatcggtctc agcaccccta tagcatcaag gaaaagcaga tgaagagtga agtgttgtcc
      781 gttaaagaga aaattggtta tggcatggga gacgccgcca gccacattat tttcgataac
      841 gtaatgttat atatgatgtt cttttatacc gatatttttg gcattcctgc cggatttgtc
      901 ggaactatgt ttttggtcgc tcgtgcactg gatgcgattt ccgatccttg catggggttg
      961 ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc cgtgggtact gtttggcgca
     1021 ctgccattcg ggatcgtctg tgtactggcc tatagcacgc cagatctcag tatgaacgga
     1081 aaaatgattt atgcagcaat tacttacacc ctacttacct tactttatac cgtcgttaat
     1141 atcccttatt gcgcattggg tggtgtaatc accaatgacc cgacgcagcg tatctcgctg
     1201 caatcctggc gttttgtgct ggcgaccgcg ggaggcatgc tttctacggt tctgatgatg
     1261 ccattggtta atttaattgg tggtgataat aaaccactcg gtttccaggg cggtatcgcg
     1321 gtcctttccg tggtggcatt catgatgctg gcattctgtt tcttcaccac taaagaacgc
     1381 gttgaagcac cacctacaac gacgtctatg cgggaagatt tacgtgatat ctggcaaaac
     1441 gaccagtggc ggattgtcgg tttactaacc attttcaata tcctggctgt gtgcgtacgc
     1501 ggtggagcga tgatgtatta cgtcacatgg attttgggta cgccggaagt gtttgtcgct
     1561 tttctcacca cttattgcgt gggtaacttg attggttccg cactggcaaa accgctgacc
     1621 gactggaaat gtaaagtcac tatcttctgg tggacgaacg ccctgctggc agtgattagc
     1681 atcgcgatgt tctttgttcc catgcaggcc agcatcacta tgtttgtctt catcttcgtg
     1741 attggtgtgt tgcatcaact ggtgacacct atccagtggg taatgatgtc cgataccgtc
     1801 gactacggcg agtggtgcaa tggtaaacgc ctgaccggga tcagttttgc tggcacgctg
     1861 tttgtgctca aactggggtt ggccttcggc ggcgctctta tcggctggat gctggcttat
     1921 ggcggatatg atgcggcaga aaaagcgcag aacagcgcca cgattagcat cattattgcg
     1981 ctattcacga ttgttccggc gatctgttat ttgctgagcg cgattgtcgc taaacgctac
     2041 tactcactca cgacgcacaa tctgaaaacc gttatggaac agctggctca gggtaaacgc
     2101 cgttgccagc aacaattcac ctctcaagaa gtgcagaact aaggaacggc aatgaaaatt
     2161 agcgatggaa actggttgat tcaacctggc ctcaatttga ttcacccgct tcaggtattc
     2221 gaggttgaac agcagggtaa tgaaatggtg gtctatgctg ccccccgtga tgtacgtgaa
     2281 cgtacctggc agcttgacac gcctttattt acgctgcgct ttttctcccc acaggaaggt
     2341 attgtcgggg tgcggattga gcattttcag ggggcgctga ataacggccc tcattatccg
     2401 ctcaatattt tgcaggacgt gaaggtcaca atcgaaaaca cagaacgtta tgctgagttt
     2461 aaaagtggca acttaagcgc gcgtgtcagc aaaggtgagt tctggtcact ggattttctg
     2521 cgcaacggcg aacgtattac cggtagtcag gtgaaaaata atggctacgt gcaggacacg
     2581 aataatcaac gaaattatat gtttgaacgg cttgatcttg gcgttggcga aacagtttac
     2641 ggcctgggag agcgctttac tgccctggtg cgcaatggtc agacggtaga gacctggaac
     2701 cgggacggcg gcacaagtac tgaacaggcg tataaaaata tcccgtttta tatgactaac
     2761 cgtggttatg gggtactggt caatcatccc caatgcgtct cttttgaagt gggatcggag
     2821 aaagtctcca aagtacagtt cagcgttgag agtgaatatc tcgaatactt tgttatcgac
     2881 ggcccgacgc cgaaagcggt acttgatcgt tatacccgct ttactggtcg tccggcgctg
     2941 ccgcccgcgt ggtccttcgg tctgtggcta accacttcat ttaccaccaa ctacgacgaa
     3001 gcgacggtaa atagctttat tgatggtatg gcggaacgca atctgccgct gcatgttttc
     3061 cactttgact gtttctggat gaaagccttc cagtggtgcg attttgagtg ggacccgctg
     3121 acttttccgg acccggaagg gatgatccgc cgtctgaaag cgaaagggct gaaaatctgc
     3181 gtctggatta acccctatat tgggcaaaaa tcccccgtct ttaaagagtt acaagagaaa
     3241 ggttatttac tcaaacgccc ggacggttcg ctgtggcagt gggataaatg gcagccaggt
     3301 ctggcgattt atgactttac caatccggat gcctgcaaat ggtacgccga caaactgaaa
     3361 ggtctggtcg cgatgggcgt tgattgcttt aagaccgact ttggcgaacg cattcctacc
     3421 gatgttcaat ggtttgacgg ttccgattcg cagaaaatgc ataaccatta tgcgtacatc
     3481 tacaacgaac tggtgtggaa cgtgctcaag gaaaccgtcg gagaggaaga agccgtcttg
     3541 tttgcccgct cggcctccgt cggtgcgcag aaattcccgg tacattgggg gggtgactgt
     3601 tacgctaact acgaatcaat ggcggaaagt ctgcgcggtg gtttgtctat tggcctttca
     3661 ggttttggct tctggagcca cgatatcggc ggctttgaga ataccgctcc ggcgcacgtt
     3721 tacaaacgct ggtgcgcgtt tggtttgctc tccagccata gtcgtttaca tggcagcaaa
     3781 tcttatcgtg tgccgtgggc ctacgatgat gagtcctgtg atgtggtgcg cttcttcacg
     3841 caactgaaat gccgcatgat gccgtatctg tatcgtgaag ctgctcgtgc gaacgcgcag
     3901 ggtacgccga tgatgcgggc catgatgatg gagttcccgg acgatccggc ttgtgattac
     3961 cttgaccgtc aatacatgtt aggcgacaac gtgatggttg cgccggtgtt cactgaagcg
     4021 ggcgatgtgc agttctacct gccagaaggt cgctggacac acctgtggca caacgatgag
     4081 ctcgacggta gtcgctggca taaacagcag cacagcttcc tgagtctgcc catttatgtg
     4141 cgtgataaca ccctactggc gctgggcaac aacgatcaac gtcccgatta cgcgtggcac
     4201 gaaggcacgg cattccagct ctttaatctg caagacgggc atgaagccgt ctgtgaagtg
     4261 cccgctgctg acggttcggt gatctttact ctaaaagcaa cacgtactgg taacacgatt
     4321 actgtgactg gtgcgggcga ggtgaggaac tggacactgt gcttgcgcaa tattgtgaaa
     4381 gtaaatggtc tgcaaggcgg ttcgcaggct gaaagtgagc aggggctggt ggtgacgcct
     4441 caagggaatg cgctgacaat tacgttgtaa tctattgaag aatgccggat acgaatggtt
     4501 tcgtgtcgta tccggtaaat aatctcacat ctggtaataa atatttcttc catcgtattt
     4561 gcgagcatga tcacgattat tcatcgcgaa aatgtgatct gtgctgaaaa atatccctta
     4621 accgccttgc tttgttttat cactgtccgg attacggaca gattttagta ttgttgtgaa
     4681 attattctgc tttatattta ccctcagaga tggcaactga agctcttcct catggggctt
     4741 cttaatctgt gataagggta aaaaatgcaa atgattacct cgcgacaaaa cagattatta
     4801 cgattccttt tacctcgaag ggaatatacg actattgtta agattgccgg ctatttaaat
     4861 gtttcggaaa aaaccattca acgtgattta cgtttacttg agcaatggct ggggcaatgg
     4921 agaataaatg ttgagaagcg tgctggcgcg ggtgtgatgt taagcgcgga gaatattgct
     4981 gatttgctgc atcttgatca tttgttggga gcagaatgtg aagagattga tggtgtaatg
     5041 aataatgcca ggcgcgttaa aatagcgtcg cagttattaa gtgaaacacc gaatgaaacg
     5101 tcgatcagta aattgtcaga acgctacttt atcagcggag cctctattgt taatgacctg
     5161 agagtaattg agtcctggct tgcgccgttg gggttatcgt tgatccgcag cccaagtggt
     5221 acgcatattg aaggtagtga agggcaagtc cgacaggcaa tggcattact gattaacggc
     5281 attattaacc ataatgagcc gcaaggtgtc gtgtattcac gtctggatcc cggaagttat
     5341 aaagcattag tccattattt tggagaagaa gaggtgttat ttgtccagtc attgttactg
     5401 gatatggaaa atgaattaag ttggtctttg ggagaacctt attacgttaa catttttact
     5461 cacatcctta ttatgatgta tcgcaacacg cacgggaatg cgttatcaag agaagaagat
     5521 caaaccaggc aatatgatga aaatatcttt aatgttgcca gtcagatgat tcataagata
     5581 gaacaacgaa ttgcacatac attgcccgat gatgaagtct ggtttattta tcaatatatc
     5641 atttcatcag gtgtggcgat tgatggacaa aaagatgtta gcattatttc acatatgcag
     5701 gccagcaatg aagcgcgtct tattacctgg cgtttaatta cggtattcag tgacatcgtg
     5761 gactgcgatt ttagtgaaga cagcgcatta tatgatggct tattggtgca tattaaaccg
     5821 ctgattaacc gactaaatta tcgtattcat atccgtaatc cattgctgga agatattaaa
     5881 gcagaattag cggatgtctg gcggttgacg caatatgtgg tgaatcaggt atttaaaacc
     5941 tggggtgaga atgcagtgag cgaggatgaa gtgggttacc tgaccgttca ttttcaggct
     6001 gcgatggagc ggcaaattgc ccgtaaacgt gtattactgg tctgttcaac cggaatcgga
     6061 acttcgcatc tactgaaaag ccgtattctg cgagcatttc ctgaatggac gattgttgat
     6121 gttatttcag cagcgaattt atcacaggtt ttgcctgaca atatcgaact gattatttcg
     6181 accattaatt tgcctacagt cactatgccg gtcgcttatg ttaccgcttt ttttaatgat
     6241 gccgatatta agcgggtcac tgaaatggtg attacggaaa aattacatca tgcgacgtct
     6301 cgggtcgttg aaatttaatg tcagaatttt atgaggtatg acgaatggac ataacaaaaa
     6361 tcctgaatac gaatcgcgtt attttagata tgcaagcgac gaataaagcg gaggcgattg
     6421 aagaattaac taatcttctc aaaaaagatg gtgcgatcga ttgtcgggaa acctttatta
     6481 aagatgtctg gcagcgcgaa gcggaaggct cgactggttt tgaaaatcat attgcgatcc
     6541 cgcatggtaa atcttctgcg gtaattaata ctacgctggc aattggccgt acccgccagg
     6601 atattccctg ggaaacgctg gatggtagta gcgtgcgctg tattatttta tttgcggtac
     6661 gtcttgaaga tcaaaatacc acccatatcc gtttactttc ccaggtcgcg ggcgcgctgg
     6721 cggatgatga catcatagaa caattgttag ttgagacgag tccgcaaaaa atcattgatc
     6781 tctttagtca gtatgcacaa tccgatgtct gctaaataac agggagtatg tcatgaatat
     6841 tgtctgtgtt gccgcctgta cggcgggtat tgctcatacc tatattgctc gtgaaaagtt
     6901 aatcaaagga gcaaaagctc tgggccatac cattaaagtt gaaacccagg gaaccattgg
     6961 tacagaaaat gaattacagg cggcagatat ctctgctgct gacgtcgtga ttcttgctgt
     7021 agatgtgaaa ataaaaggag aagaacgctt caccaataaa cgtattgtgc gagtaaagac
     7081 agagatcgtc attaaatcgc ccgttcaatt tcttgaaaag gtcgaaaaat cactgggaaa
     7141 caaatgatcc ttatactgga gggatcttaa tatgaaagac aataaaattc cggtttcaca
     7201 ggaaattaaa aaacatttat taaccggcat ctcgtggatg atacccctta ttgtggccgc
     7261 cgggatatgt attgcgttgg ggcaagttct tggcggtaca gatgttggtg aaaaaacagg
     7321 aacaatcccg tggatgctta atcagattgg cggctgggga atggggctga ttgtgccgct
     7381 gattagcgcg gcaattgcct attctattgc cgatcgtccc ggatttgccc cgggtctgat
     7441 tgtcggcttt ctctgtgggc aaatccatac cggatttatt ggcggtatgt tgggtggttt
     7501 cctggtgggc tacaccattt tgctacttaa acgctatatc cgcctgccgc agtcgatgca
     7561 aggactcatg ccaatcatgg tgttgccggt attaagcacc attatcggtg ggctgttaat
     7621 gatgacgctg attggtaaac ccatcgcctg gttacaagaa gcactgattc atttgctcga
     7681 gtcgatgcag ggcggatcgc gcttcctgat gggggccata cttggcgcga tggcaacctt
     7741 cgatttcggt ggtccggtca acaaaaccat gtcgctgttt tccgatggct tgctggtcag
     7801 tggcgtctac ggaccagaag ccgtgaaatt tgtcggctca atcatccctc cgtttggcat
     7861 cacactttcg ttcttactga cacgccataa atacacccgt gcggagcgcg aggcgctgaa
     7921 agccgcgttt ccgatgggga tctgcatgat cactgaagga gtcattccga tcgccgcgcg
     7981 tgacctgctg cgagtagtgg gatcttgtgt ggtggcatcg gcggtcgcgg gcggcttaat
     8041 catggtgtgg ggcgtggaga gtccggttcc gcatggcggc atgtttgttg tgcctctgtt
     8101 cacccatcct ctgctgtttt gcctggcgct gggtataggc acggtgattt gtggcgtgat
     8161 gctttcactc tggaagaaac cggtaacgga acgcgacgaa gagtttgacg aactgagcga
     8221 tcaaaagctg aaagatgaag agatcacttt caccctcgaa taaccggagg ccctatgttt
     8281 gccgatatga aaagtatggt gatgaaagcc tggcatgaac attacgcctt gctggcgatc
     8341 aactgtatga atctggaaag tgcttacgca gctatccgcg tggcggagaa gaatcgcgct
     8401 ccgatcattc tcaatctcta tcaggggcat ctggcacatt ttcctgcacc cgtcgcggcg
     8461 gcagtggtta aaacgctcgc ggaatctgcc tcagtaccgg tcactctggc gcttgatcat
     8521 ggcaaaaatc cggactgtat tcgtcaggcg tttcgtgctg gtttcagcgg attaatgatt
     8581 gatgcttctg cttttcccct ggaggaaaac gtgcgccaga ccagggcggt ggtggaactc
     8641 gccgccagcg cgcagctctg cgtggaaggc gaactgggcc atctggcaga tgcgccgcgt
     8701 tacgaccagg ccgccaatgc agacatgatg acgcaaccca ctgatgtgga accgtttatc
     8761 gcgcagacag gcattgatct actggctgtc tctgtgggta cggcgcacgg catgtacgcg
     8821 cctggtgttg tacctgctat tgattttcag cgtctggcgg agattttcca ctgctcgagc
     8881 gtgcctctgg cattacatgg cggtagcggt acgccgttcg atcagctcca gctctgtacg
     8941 acgctggggg tagcaaaaat caacgtgggg gcggccattt ttgaacgggg gaaatccgcg
     9001 ctcctgcata ccctctgtca cgatatatcc attgaactgg tggacgcgct gaaagctatg
     9061 gaactggcct tcgaagaagc gattacaccg tatttacagg cgagcggctc tatcggcaaa
     9121 gcctgattca ttttctgtca tggcctttat gccagtgcaa taacgcaccg gcagggcgga
     9181 ctacacgctg aaactggaga tttaacatgt tagtttcgat gaaagaatta ctgcaaccta
     9241 cccgccagca cggctttgcc attggcgcat ttaatgtggc ggataactgt ttccttcgcg
     9301 cagtcgtgga tgaagcagaa gcgacaaata ctccggcgat tattgccatc catcccagcg
     9361 aacatgattt tgtgggcgat gcctttttcg ctcacgttcg cgaaattacc cagcgtagtc
     9421 cagtcccgtt tgtactgcac ctcgatcatg gtgcctctgt agagcatgtt ttgcgtgcga
     9481 ttcgctgcgg atttacctca gtaatgatcg acggttcgct gctgccgtat gaagaaaacg
     9541 tggcgctgac cgcagaagtg gttcgcctcg ctcacgcggt tggcgtgtcg gttgagggtg
     9601 aactgggaac catcggacaa acaggaacgt cggtagaagg tggtgtttca caagtgacct
     9661 acacagatcc ggcgcaggcc gccgatttta ttgctcgtac aggtgccgac acactggctg
     9721 tggcgattgg tacagcgcat ggcatctatc caaaagggat gcaaccgaag ctgcaaatga
     9781 acattttgcg cgacattgcc ggacgtattg atattccgct ggtactacac ggtggttcgg
     9841 caaacccgga cgcggagatt gctgaatcag ttacgctcgg cgtcggcaaa atcaatatct
     9901 ccagcgatat gaaatacgcc tatttccaga aagtgcgtga aattctcgct aaagagagct
     9961 ggtgggaccc gaacgtcatc tatccggatg cgattaacgc ggcgcgggaa gtcattcgcc
    10021 acaaaatgaa actgttcggt tcgctcggta aagcatcact gtactaagcc agaagggcgg
    10081 ggtgacccgc cttttacgca acaaggaacg agatatgcac tatctggggt tggatattgg
    10141 tgggaccaaa atcgccgccg tcgtcatgga tgcgcatggc tgggagattc gccgttaccg
    10201 ctgcccgacg caaaagtcga catatcaaca atttgtctca tgcgttgtgg cacttatcga
    10261 gcagattagg cgggacgttc aacgaccgat gctgacaggg atcgccttac ccggcagtat
    10321 ctcgccactc actggcctga ttaaaaacgc gaatattcag gtgattaacg gtcatgcgtt
    10381 acaggctgat ttgcagcaat tgcttgggca accggtggtg atagccaatg atggtaactg
    10441 tttcgcgcta tcagaagctt gcgacggtgc cgggcaagat tatgacgtgg tatttggtat
    10501 tacgcttggt acgggctgcg gcggtggcat tgccatcaag caacgaccgt ttataggggc
    10561 ctggggaaat gctgccgaat gcggtcatat cacgttgcca ggctatacgg agcaggaaga
    10621 tggtccatca gtcagttgct attgcggcaa acacaactgc gtggagtcgt ttgtttccgg
    10681 cagcggtttt agtgaacgct atcaacagat gactggtaac ttgctcactc ctgcggcgat
    10741 tgtcaccctg gcacaacgtg gtgatgcttg tgccatgcag caggtggcac gttttcgcca
    10801 acagcttgcc cgcacgctcg caaccatcgt taatgttgtt gaccctggcg tgattgtgat
    10861 cggcggcggg ctttcgaatg tggaactgct tatcaccgat ctgaacgcag aagtcgctcc
    10921 actggttttc accgatcaat tcaccactcc cattgtaaaa gcacagcacg gcgacagtag
    10981 cggaatgcgt ggcgctgcct ggcttgctat gcgcaacgga gaagccaatg aaacgttcac
    11041 aaattaatta tgttattgat aaagcacatg ccattgcgca gaccttccgc gtttgtttgc
    11101 cagagttcgc ctattttacg gtggatgact ggctgcagcg ggaacgggat aactggcaag
    11161 aagtggtcga cttacagctc ggttgggata tcaccgattt tggtcgtggt gatttcaacc
    11221 aaacgggact aacactgcta acgatgcgca acggcgcgct ggggtcggcg gcatatccaa
    11281 aaccgtatgc tgagaaaatg ttgcagatcc aacaagatca gcagactccc tggcattttc
    11341 acacccacaa aatggaagat atcctcaatc ggggcggtgg agacttatgc atgcagttgg
    11401 cctgggccaa cgaagccagt ctctgtgatg atcaacgagt cattaccgtg agtgttgatg
    11461 gtcagcggcg taccatgaaa cctggtgaaa cgctggtgtt aaagccgggg cagggcattt
    11521 gcctgccgcc gcgtctgtat catcgcttct gggcggaaaa agcgtttgtt ctcggctggg
    11581 aaatttcgat ggtaaatgat gaccaacacg ataactattt tcttgagccg ggaggacgtt
    11641 ttcctgcgat agaagaagat gaaccggtga aatggttatt gtgcagtgag tatggaattt
    11701 tgcgctaata acggggaacg ctagcgctga tatccgctag cgtccttatt tttactgcgc
    11761 cggctccgtc gacgtaactt caccactgga aactacgccc gcattcatgg tttgagtcac
    11821 ttgctgcttt gcggcgttca cggcatgcaa ttgcccgcta accgtaggtt tcagcgggac
    11881 attggcctga atatcgccac tggcggtaag ctgaatattg ccgtcgccag tcaacggtaa
    11941 ttcaggccat ccccaatgtt gcaaaatatt caccggcaca ccgcgtccat tcaggctgat
    12001 atgggtctga cgttgtggcg tttgtgaaac actggcggtg gcttccagaa tgcctttttc
    12061 agtaaatgca ctcagttcgc tgatattcac cgtgctgctg ttggcggtta gcgccagcga
    12121 tggacgacgg acatcaacac gattaaacgt cgcggcggcg gcattcagat tcgccgaacc
    12181 actccagacg ccccatttat gatcggtaac cagcgtcagg ttggcaccgt aaccatcgag
    12241 cgcggtgagc tgccacggga agtcagggtc gatatcaatg atcagattgc ggctggcgct
    12301 aaatctcttc agttgcaggc tgtttaacca acccggtgtc gtttccatcc acaactgttg
    12361 ccagtttttc ggcaaggtat attccagccc ggcaattgcc gcatcatcaa ggagtaacgt
    12421 tttcccgtca cgcagccagt tccctgacgt tctgaccata cccccttccc agcggctggt
    12481 gaactggcgc agcgctacgc cctgcgggga aaattccgcg tttataatcg ggtcaaataa
    12541 atgcagcgaa ccataaatga actcgctagc gttcatcgac agtttgcctt cttgtgtctg
    12601 ccagtcatct ttactgaagg tcatgttgcg caagctgaga tcgaggtcgg tcaccgccca
    12661 gtccggacct tgcagacgag catcgatcac ttccaggcga ccaatctgca acgacgggac
    12721 agagcgtaat ggtgcaaaga agtcggttag cgatttttcg ctttgtagac ggatatccgc
    12781 catgcgcaga ttttccactt gccagctgcc gtcggcgtta cgctgcgcgt ttccggttaa
    12841 tgtcccgcgc gcgatgtcgg cacccaggtt agtcagcgta acgcgatcgt tatcaatact
    12901 gccttcaatc agtacattgg tggcaggaac atcattgagc gaaagcgatc cggcactaaa
    12961 ctgaatctgc gccttcgtac ccagcacttt accggcttct ggtgaccacg gaaccacgcc
    13021 gccatttacc cgctgcgcgc tcagtttcca ttcgctattc gggctattaa acgccatatc
    13081 acgcagttgc agacgatcgg ctttgaacgg tagcggcgcg gtctggtcgg tgagattcag
    13141 cgttccgttt tccagcagga tggtatcgac atggcgtggt tcggtcagtt gccgactact
    13201 tagcgcaatg tcgacacttt tggccaccag ggtcgcgggc tggccatcac gaccaaacgt
    13261 gacgttctcc agcacaatat gagatggcgc ggaaaaacgg tgatccatcg ccccgaaggc
    13321 cagatgatag tcgctattct cggaaaccca tgcgctgata tgttctgctc cccagcgggt
    13381 ttgcagaaga aaatagaggc cagcgatcac cactaacaga gcgatgagaa tgtagagaag
    13441 cagcttccca ataaatttca tggtcttcca tcccgtgaaa tgcacataag ggagttatgc
    13501 acgatttacg cgcaatcctc aaggcgggaa tggtgaaaga ggtgagactg cggcggtaat
    13561 tatcttaccg ccgccagtga attattgttt ttctggtggg aaaatcagat tcagcacgat
    13621 ggcagtaata ccgcccgcgg cgatcccgga ggagagcagg tttttcagcc attcaggggc
    13681 aaactgcaaa atcagcggct gctgagacac gcccagacca accgccagcg acagggcgat
    13741 aatcagaatc gcccgacggt tcagcggctc acgagaaacg atacgcacac cggaggcggc
    13801 gatggtgcca aacattacaa gcgttgcgcc gcccagaacc ggttctggaa tgtgttgtac
    13861 aaaaccgctc actgccggga acagacccag cacgatcaac atcagcgcga cgacaaaacc
    13921 gacatagcgg ctggcaacac cggtcaactg gatcactccg ttgttctgcc cgaagcagga
    13981 gttcgggaag gtgttaaaca ccgccgaaac aaacgagttc aggccgtttg ccagcacgcc
    14041 gcctttcagg cgtttcatgt acagcggacc ggacactggc tgttcggaaa cgtcagaggt
    14101 cgccgtgata tcgccaatgg tttccagcga agtgatcata aagaccagca tcagcggcag
    14161 cagcagactc cattcaatgc caagaccgta atagagcggc gttggcacca taatcagttc
    14221 ttgcgtcatc ggttcgttgc tttctggcaa catgcccata aaccacgcca gcgcatatcc
    14281 ggccgccatc gcaatcacca gtgaggccac gcgtaagtaa gggttacgtt gacggttaag
    14341 caggataatt aaggctaaga ccacgcctgc cagcagcaga tttttcggtg cgccgaaggt
    14401 gttatcgctc atggctgcgt aaccgccgcc aattgaggtc agccccacct gaattagcga
    14461 caggccgata atcatcacca caacgccaga aaccagcggc gtaataatgc ggcgcgccag
    14521 atgcagaacg cgggagatca ccatctcggt gcaacttgcc agcatcaacg tgccgaacaa
    14581 agccgccatc atggtaggaa catcagcacc accggttttc agcgcggtac cgcccataat
    14641 cagcggggca acaaagttga agctggtgcc ctgaatagac aacagcccgg agccaaccgg
    14701 accccaggcc ttaatttgaa taatcgatgc cacaccggag gcaaacagcg acatactaat
    14761 aatgtgttgc gtgtcttgtg ccggtaaacc cagcgcctgg cagattaata gcgctggcgt
    14821 gatcaccgca acgaacatcg ccagcaaatg ctgacaggcg gcaaacaggg tttgaggaag
    14881 cggcggacga tcttcaagac ggtaaatcag ttcgctgttt tgagtctgcg caaccggttg
    14941 cgcattttct gactcgaggg tggaaacaga catcgaaaac aatcccgtgg tagcaaagcg
    15001 ggcattttag ctgaccgaaa gagaaaagca aacgtttgcc acatcctcat ctaaatattt
    15061 aaatgagata aatgcacttt tttatataac ttttggtatt tttctgctta aaatccatgc
    15121 catttgttca ttaacagctg agaatttgac ccaggaaagc ataactcgat acccgtcata
    15181 gttcataagt tgccactgcg cgatgaagta tgacgagtat gaaagagtga tgcggataca
    15241 aaggagtaac tatgtttcat ctcgatactt tagcaacgct tgttgccgca acgctgacgt
    15301 tgctgctcgg gcgtaagttg gtccattccg tctccttttt gaagaaatac accataccgg
    15361 aacctgttgc gggtggtttg ttggtggcgc tggcgctact ggtactgaaa aaaagcatgg
    15421 gctgggaagt caactttgat atgtccctgc gcgatccgtt aatgctggca ttcttcgcca
    15481 ccattggcct gaacgccaac attgccagtt tgcgtgccgg tgggcgtgtg gttggcatct
    15541 tcctgattgt ggttgttggt ctgttggtga tgcaaaatgc cattggcatt gggatggcta
    15601 gcctgttagg gcttgatccg ttgatggggc tgttggccgg ttctattacg ctttccggcg
    15661 gtcacggtac gggagctgca tggagtaaat tgttcattga acgttatggc ttcaccaatg
    15721 cgacagaagt ggcgatggcc tgtgcaacgt tcggtctggt gctgggcggc ttgattggcg
    15781 gtccggtggc gcgctatctg gtgaaacact ccaccacgcc gaacggtatt ccggatgacc
    15841 aggaagtccc gacggcgttt gaaaagccgg atgtgggccg catgatcacc tcgctggtgc
    15901 tgattgaaac tatcgcgctg attgctatct gcctgacggt ggggaaaatt gttgcgcaac
    15961 ttttggctgg cactgctttt gaactgccga ccttcgtctg tgtactgttt gttggcgtga
    16021 ttctgagcaa cggtctgtca atgatgggct tttaccgcgt ctttgagcgt gcggtatccg
    16081 tgctgggtaa cgtaagcctg tcgttgttcc tggcgatggc gctgatgggg ctgaaactgt
    16141 gggagctggc ttcgctggcg ctgccgatgc tggcgattct ggtggtacag accatcttca
    16201 tggcgttgta tgccatcttc gttacctggc gcatgatggg caaaaactac gatgcggcag
    16261 tgctggctgc gggccactgt ggttttggcc tcggtgcaac gccaacggca atcgccaaca
    16321 tgcaggcgat cactgaacgt tttggcccgt cgcacatggc gtttctggtg gtgccgatgg
    16381 tcggtgcgtt ctttatcgat atcgtcaatg cgctggtgat taagctgtat ttgatgttgc
    16441 cgatttttgc gggttaaccg atgaagcggc ggtagaagtg ccgccgcaac aaagacaaat
    16501 gcctgatacg cttcgcttat caaggctgcg cattgattca caatttattg aatttataag
    16561 tttttgtagg acgaagcaca tccggcagga aggtagggta acctgaaatg gcggtcttct
    16621 cactgccgcc ttttacgcat tcgagtaacg ttccgtctcc ggcatccagc gttctatcag
    16681 ggcttttgcc tgttgtgggt aacgttcgtg aatatggcgt gccaggcgct gaacttccgg
    16741 gatcatcgcc tgatcgcgca gtaaatccgc cactttaaat tcagcattac ccgtctgacg
    16801 agtgcctaac aattcgccag ggccgcgaat ctccagatct ttttgtgcaa tcacaaaccc
    16861 gtcgttgctg tcgcgcagca cttgcaggcg aatttgcgcc gttttagaaa gcggcgtttt
    16921 atagagcagc acgcagtgag aagccaccgc gccacgacct acacgcccac gtagctggtg
    16981 caactgcgcc agacccagac gctccgggtt ttcgataatc atcagactgg cgttaggcac
    17041 atcaacgccg acttcaataa cggttgtggc aaccagcagg tgtagctcac cttgtttaaa
    17101 cgacgccatc accgcctgtt tctcggcagg tttcatccgc ccgtgtacca ggccaacatt
    17161 caactctggt agcgccagtt tcaactcttc ccaggtagct tccgccgcct gcgcttccag
    17221 caattccgac tcttcaatca acgtacaaac ccagtacgcc tgacgacctt cagtcatgca
    17281 ggcgtggcgc acgcggtcaa tgatgtcggt acggcgagta tcaggaatag cgaccgtagt
    17341 cactggcgtg cggcctggcg gcagttcatc aatgaccgag gtgtcgagat cggcatacgc
    17401 agtcatcgcc agcgtgcggg ggatcggcgt ggcggtcatg atcaactgat gcggatggaa
    17461 gccttgctgc tggcctttct cccacaatgc cagacgctga tgcacgccaa aacgatgctg
    17521 ttcgtcgata atcaccagcg ccagaccgtt aaactgcacc tgttcctgga agatggcgtg
    17581 cgtaccaaca atcatctgca cctgaccgct ggcgatggct tcctgctgag acagtcgcgc
    17641 tttacctttc tgcttaccgg cgagccagcc cacttcgata ccgagcggtg caaaccagtt
    17701 gcggaagtta ttggcgtgct gttcggcgag taattcggtt ggtgccatca atgccacctg
    17761 tttgccgtgg gcaatcgcac gcagcgcggc gagggcagcg accagcgttt taccggaacc
    17821 tacatcgccc tgtaccaggc gcatcatcgg cacattcagc gccatatcgc gctcgatctc
    17881 cgccaccacg cgagcctgag cgcccgttgg cttgaacggt aaggcggcaa gaagtttatt
    17941 tttcagcgtg tcattggcgc tcagcggctg ggcatggaaa cgttgcgctc cggcgcgtaa
    18001 ggctaacatg ctgaggttat gcgccagcag ttcttccaga atcagacgac gttgcgccgg
    18061 atgctgcccg gtttccagat cgctaagttg cagcgtcggc ggcgggcggt gcaaagtgcg
    18121 caacgcttcc ggtagcgtca tcattccttg tgacagttcc ggcggcagga gttcttcaat
    18181 ggcgcaggtg tcgagcagat ccagcgcctg gtcggttaat ttacgcagcg tggcctgctt
    18241 tacgccttcg gttgttggat aaaccggcgt gagcgtttcc tgcaactctg gcgtactgag
    18301 atcgccctgc acgcggtatt ccgggtggat catctccgca ccatatttac cgcgctttgc
    18361 ttctccatag gccaacacgc ggcggcccgt cgccaggcta tttttcattg ccgcgttgaa
    18421 gttgaaaaag cgcatggtga gaatgccgga accgtcgctg atctggcagg tcatcatccg
    18481 ccgaccgccg aaggagatat tgcagttcag cacttcgcct tccaccgtgg cataaacgcc
    18541 cggtagtagt tctccgatgg ggtagagatg ggtgcgatct tcgtagcgca ggggaaggtg
    18601 taagagtaga tcctgcacag tctgcaggtt gattttcgcc agcttgttac taagcgctgc
    18661 gccaacgccc gttagggaac tgagcgggac agcatctaac aggcgacctt tcatggcact
    18721 taccctgcag cctgcatggt agcccaccag tcggcatcag cttcgatctc gccttgctga
    18781 ttgacgtggg ggtaaggcag gcctttgcgt tttgcgactt tcgccagcac cggatagccg
    18841 ccttcaaaca acaggcgttg ttgctctgct tccggcaaca tgctgttttc acgcaggtac
    18901 atgcctgcat tttgccgctg acgctgggct tcgtaaagaa tgagggctga ggcaacggaa
    18961 acattcagcg actgcaccat gccgatcatc ggaatgatga tgtcctgatc tgccagggcc
    19021 aacgcttcct cagtgatgcc cgttttctcc tgacccatca aaatgcaggt cgggcgagtg
    19081 taatcaattt cgcgaaaatc gacagcgtta tcagaaagat gggttgccag aatttgcatg
    19141 ccctggcctt tgagatgagc gacggcatcg ccaatggtgc ggtgcgtttt cacctgcacc
    19201 caactgttgc tacccgccgc cgcagaggcc atggtacgca tgcggctacc aggccagacg
    19261 gcgtgaactt catgtacgcc aacggcatct gcggtacgaa taatcgcaga aacgttatga
    19321 ggtttgtgga cctgctccat gcagacggtc agatcaggct gccgcctggc gagcatttcg
    19381 cagatgcgtg cataacgtgt tgggttcata aaacattaat ttcggtttcg ggtgacttta
    19441 atcacgtctg gcatcacgcg gattttgcgc atgatattcg ccagatgcac acggtcacga
    19501 gcggtcagac gaataaaggc gctgtagacg cgaccatctt tctcttccgt attcaaactt
    19561 tgaatattcg aagtcgtggt gttaattgcc gccgtcaggt ttgccagcgc gccctgatga
    19621 ttgaacatct ccaccttgat ttcggtgatg aactcctgcg ccgtctcttt atcccattcc
    19681 acagccataa acttctctgg ctctttctgg tagccacgga tgttacggca ggattcatgg
    19741 tggatcacca gacctttacc ggggctgacg tgggcgataa tcgggtcgcc aggaataggg
    19801 cggcagcatt tcgcaaaggt gatcagcacg ccatcggcac ctttaatggg cagatgtccg
    19861 tgactttggg ttgccggtgg aatggaggcg tccccatgtt gcagattttt cgcgaccacc
    19921 acgctcattg cgttaccaag tccgatttct gccagcagat cgtcaagcgt tgccagcttc
    19981 atacgatcca gctcgcgctg aatattttcc tgcgggattt cattcagctt acggctacca
    20041 cccaaagcat ggttgagcag acgacggccc aggcttacag aatcatcacg cttgaggttt
    20101 ttcagcaact gacgaatttt ggcgcgcgct ttcgagctaa cgacaaagtt cagccacgcg
    20161 gcattcgggc gagcgcccgg tgcggtaatg atttcaacgg tttgaccgct agtaagcggc
    20221 tgcgacagcg ggtaaggctg gcggtcgacg cgtgcgccca cgcaggcatg accgatatcg
    20281 gtatgcactg cataagcgaa gtcgacgggc gttgcaccgg caggcagttc gacaatgcgc
    20341 ccttccggtg tgaaaacgta aatctcatcc gggaagagat cggatttaac gctctcgata
    20401 aattcaaacg agctaccggc gctttgttgc agctccagca ggctttgcat ccagcgctgg
    20461 gcgcggattt gtgcagtcgt gctggtttcg ccgtgctctt tataagccca gtgcgcggca
    20521 acacccatct ccgccatctg atccatatct tcggtacgga tttggacctc aaccggcacg
    20581 ccgtgcgggc caatcatcga ggtgtgcaac gactgatagc cgttcgcttt tggaatagcg
    20641 atgtagtctt tcacgcgacc cggacgcggc ttatacaggc tatgcatctg gccgagcacg
    20701 cgataacagg tgtcggaatc attgacgatc acgcggaaag cgtagatgtc catgatcgag
    20761 tgaaaacgct gctctttgag caccattttg cagtaaatcg aataaagatg cttctcgcga
    20821 ccactgacgc ggcacggtat tcccgcttcc tgcaaacgcc cttcgatttc agagaggatt
    20881 ttttggatca tctctttacg gttgccgcgc gcggctttca ccacttcttt aattacgcgg
    20941 taacgattgg gatacagcgc ctcaaaaccc agctcttcga gttcggtttt aatgtggtgg
    21001 atacctaaac ggtgcgccag agggctataa atttcgagag tttcacgggc gatgcggcga
    21061 cgtttgtccg ggcgaagtga gcccagcgtg cgcatgttgt gggtacggtc ggcaagtttg
    21121 atgagaatga cgcggatatc ctgcaccatc gccataatca tcttgcgaaa gttttcggcc
    21181 tgcgcctctt tcttatcgcg gaacttgagt ttatcaagtt tcgacacccc ctctaccagc
    21241 tcggcgacgc ttttaccaaa aagctgttcc atatcctggt aggtggcggg agtatcttca
    21301 atcacgtcat gcagcagcgc tgccatcagg gtttcatagt cgagtttcat ctcggccaga
    21361 atgcaggcaa cagctaccgg gtgcgtgata tagggttcac cgcttgaacg tgtttgcccc
    21421 tcgtgagcat cacgtgcaac gagatacgcc tgccgcagac gcttgatttg gtcttccggc
    21481 aggtagtttt gaatcagctg attcaggctt tcaaacagat acaagggcga cccgctttgt
    21541 gattaacgac gaccttcagc aatagcggta acggcttgta attcagcggc ttcctgctct
    21601 tgctgttcct ggcgttcgcg aacgtcgagg atctggttgt tgatcagacc ttcttcgatt
    21661 tcgcgcagcg cgattacagt ggttttatcg ttttcttccg gtaccagcgg atcctttccg
    21721 cctacctgca tctgacgagc gcgacgcgcg gcgaccagta ccaggtcaaa acggttacca
    21781 attttctcta cagcgtcctg aacagttacg cgtgccatac ttaaaaagct ccacaggtga
    21841 agaaatgact gggcatgata ctgaaatcag gttcagtctg ccaacaattt gctgattaaa
    21901 gcgtcatgac gctgcttttg gcggctcatg cgcagacgtt cggcgcgaat aatggtcttc
    21961 aaatcggtca acgcggtatc gaagtcatca ttcacaatca gataatcata ttcggcgtaa
    22021 tggctcattt ctgcaacagc ttgcgccata cgctttgcaa tgacctcttc gctgtcctga
    22081 ccgcgaccgc gtagacggcg gtccagttca attttggacg gcggtaaaat aaagatactc
    22141 cgcgcgtgcg gcatcttctg gcgaatttgc tgcgcaccct gccagtcgat atcgagaaaa
    22201 acatcgacac cggtcgccag tacttgctca atggcctcac gcgaagtgcc atagtaatta
    22261 ccaaaaactt ctgcgtgttc gaggaacgca tctctgctaa tcatttcttt aaattcatca
    22321 tgattaacaa agaaataatg ttcaccgtgg acttcaccag gacgcggttg gcgtgtggtg
    22381 tgtgaaacag aaacttgggt gtcatacaac ggttgggttt ttaataaagc ctgaatcagg
    22441 ctggatttac ccgcgccact gggggcagaa acaatataaa gcgtgccttg agccatgagt
    22501 atctttcgta tgtgattagc gaaataaagc ctacatacga gcttattata cacggcgcgg
    22561 ccatgtgacg tagcccttgc tcacactttt tgcgcaaagt tgttgcgttt tgcgtagctg
    22621 cttccgcatt tgccagtgat tcactgcaat aaagacgatt ctcaggaagc gtcctcgcag
    22681 actccgtttt tgccactcgg ctttgctttc atcacgttgt atatctgcct gactatctac
    22741 agcggaggtg atgatgaaag tatggatggc gatattaata agtatcttgt gctggcaatc
    22801 atctgtgtgg gcggtctgtc cggcctggtc gccagccaga gcacaggaag aaatttcccg
    22861 cctgcaacag caaataaaac agtgggacga tgactactgg aaggaaggaa aaagtgaggt
    22921 ggaagacggt gtttacgatc agttaagcgc ccgtcttacg cagtggcaac gctgctttgg
    22981 gagcgagccc cgcgacgtga tgatgccgcc tttaaatggt gcggttatgc atccggttgc
    23041 ccatacgggt gtacgcaaaa tggtggataa aaacgcatta agtctgtgga tgcgagaacg
    23101 tagcgatctt tgggtgcagc caaaagttga tggcgttgcg gtaaccctgg tttatcggga
    23161 cgggaaactg aacaaagcaa tcagtcgcgg taatggcctg aaaggcgagg actggacgca
    23221 gaaagttagc ttaatttccg ctgtgccgca aaccgttagc gggcctttag ccaacagtac
    23281 gcttcagggg gaaatatttc tccagcgcga ggggcatatc caacaacaaa tggggggaat
    23341 aaatgcccgc gcaaaagttg ctggcctgat gatgcgccag gggaatagcg acacgctaaa
    23401 ctcactggct gtttttgtct gggcatggcc ggatggaccg cagttaatgt ccgatcgttt
    23461 aaaagagctg gctaccgctg gttttactct aacgcagacg tatacccgtg cggtgaaaaa
    23521 tgctgatgaa gttgcgcgcg tacgcaatga gtggtggaaa gcgaaattac ccttcgtcac
    23581 cgatggcgta gttgtacgag cggcgaaaga gccagaatcc cgccattggc taccgggcca
    23641 ggcagagtgg ctggtagcct ggaaatatca acctgtagct caggttgccg atgtgaaggc
    23701 gattcagttt gcggtgggta agagcggtaa aatatcggtg gttgcgtcac tcgcacctgt
    23761 catgctggat gataaaaaag tccagcgggt gaatattggt tccgtcaggc gctggcagga
    23821 gtgggatatt gcacctggtg atcagatcct cgtcagcctt gccggtcagg gtattcctcg
    23881 cattgatgat gtggtctggc gcggtgcaga acgtacaaaa ccgacaccac cagaaaaccg
    23941 ctttaactcg ttgacctgct actttgcttc tgatgtttgt caggaacagt tcatttcacg
    24001 cttagtctgg ctggggtcaa aacaggttct ggggctggat ggcattggtg aggccggttg
    24061 gcgcgcgctg catcagactc atcgctttga gcatatcttt tcctggcttt tattaacgcc
    24121 agagcaatta cagaacacgc cggggatcgc gaaaagtaaa agtgcgcagt tatggcatca
    24181 gtttaatcag gctcgtaagc agccttttac tcgctgggtg atggcaatgg gaataccgct
    24241 aacccgggcg gcgcttaatg ccagtgatga acggtcctgg tcgcaacttt tagttagcac
    24301 ggaacagttc tggcagcagc tgccggggat tggatctggg cgcgccagac aggttattga
    24361 atggaaggaa aatgcgcaaa tcaagaagct gggcagttgg ctggctgccc agcagatcac
    24421 aggttttgaa ccttagtgac cttcgtggct gtagtaaaaa accggtaatc ccagcttaag
    24481 acgtagcgcc agtaaacggg caaaaaagcc gaataccagg gtggagataa tcaccacatc
    24541 atggttagaa acatagtgtt gcagcgcgat gtacagcacg gcggaggcaa atgagacacc
    24601 ggcatacaac tctttctgga ataccagtgg gatgcgttta cagaacatat cgcgcaaaac
    24661 gccgccaaac acgccggtgg ttaccgccgc gacaacggca ataattgggc cgtgccccat
    24721 atccagtgca acctgtgcgc caatgataga aaagaccacc agtccgagcg catcgagcac
    24781 cagaaacact ttgcgcaggt aaggcattac aggagcaacg atagtggtga gtactgcggc
    24841 ggtagcgacg ataatcacat attccgggtg tttgacccag ccaagcggat agtggcccag
    24901 cagaatatcg cgcactgacc ctccgccaat tgcggtggcg gtcgcaataa taattacgcc
    24961 aaatgtatcc atgcgccgtc gcccggccgc cagcgccccc gtcatggctt cggcagtaat
    25021 gccaaccaaa tacaaaatgt gtaacagcat ggttctctcc ctaaaaatct gtcttgcagg
    25081 gtagcgattt gtgcgcattg tcacgattga gattttttaa ggtgaggggg aattgataac
    25141 ttgtagttat gtgaaatcgc tattttctgt agcggagatg cattcttctg acttcttgtc
    25201 tgagaaaatg cataagaaaa aataggttaa aactgagggg tggaaaagaa aggtgcaatg
    25261 tttagctatt gtattcaccg cacctggact ttggcttacg catcgggtgt ctcaatcaac
    25321 aaaaagagtt tttagtttct gctatttctt ctctgtaatt tttacacagt tttccaactg
    25381 cttgatgctg atctgcgggg tcggcaactc ttcaggcatg gttccgccaa gttcctgaat
    25441 ggtttgtctc accttgcgac ccacgtcaaa gtgtgtggtg ttagcttgct gttttgaatt
    25501 cacgccatcc cgcttgagtt tttcttctgt ttgggtagct cgaaagagat tagccgccag
    25561 ttctgttgaa cccatatgat ccaggatctt ctgactcttt ttcagcccct tccgctgatg
    25621 aatagctttc tgatctaatc caccatacag cccccggtaa ccatgattct gaaatatggc
    25681 gaaatcgata gctgtcgcta ccccagcttg ctgcgctgcc tcaactaatt gtttgttatg
    25741 ttctttcaat tcatttctca gaaaaagacg cttttcgtct tcacgaagtt gcctgaaagc
    25801 ttcatcgtca gccagctcct gccgtcgggt ctggatagca aaataagttt gccctgccgc
    25861 aataaccggt ttcgcagggt cgccgttttg tactaccagg taacaggcat agcgggagag
    25921 atgaacatct tcaagttctc gttgagcccc tgaacctaat acaaccattt tggtggtttc
    25981 aacgaaatgg tcagatgcag cttgattgct ggcttcgcac gcttgagtcg cgcgggccag
    26041 taccttttga aaattgcgcc agtcccgata atccagtaac ggtgccaatt ctctcgctga
    26101 ccaaaattct tgcccttcag tgccataatg ccgtatttct tcaaaaggtt gatgatgttc
    26161 gttcattgcc cactccatgt gatatcgatt gcaaagaaag taaatctgta actgtattta
    26221 tatacagttg tgagtatctc acgttctcag tgtgctgggc aaaaagacat cgcaaaatct
    26281 ggaacaggca cgcaaaacag aattgaaagt tgcggagaag aaaaatgccg ctctgtttga
    26341 gtgggcggca tttgtgaagg ctgaaaagta agaattggcg ttacgagtta ttcaatgttg
    26401 aatctgttca ttgagtatct gcatcaaagt tgcttcattg cttgggtttc tgtaatgaag
    26461 attttgattg tgtacaccct tgtgtacacg attttttcag gtgtagcgcc gatatggtga
    26521 ctattagggc aaatactcag cgtatttttt ccggttatcc gttatgaagc tggtgggctt
    26581 tacctgtttt tatccgactg tgtcacataa cttttgttcc ggcatgataa aatcgtctgg
    26641 tcatatccat tactcattgt tgacctcttg ttgtgtttct ttggtggcat gccgtagtgt
    26701 tatcgcacta cggttttttt gcccgtcaga ctgaaaggga ttttttccgg ttaattacac
    26761 ttccgcctga tacatccgtc tacccaatgt gtggtaagca cctcatacag caaacgaggc
    26821 aatcggtatt ctggtccttc atggcgtttt tagcgccgtg gtgaataaca gaatgacgga
    26881 acgatagcct atggatactc acgacaagat accaccagca tattcatact ccccgcaacc
    26941 agtaccggaa gcctggaaaa tgcgattcag cgctaacagt gcgtggttct tcaccgatga
    27001 tttaaccgtg gttgatgcct ggctaaaggc ccacagcgac ggcgtggagg ttgtaccgct
    27061 gttttgtggc tctgacagtg acggtaagcg ggatgccgca tttgaggaag tgtgcgcggc
    27121 tcttgcccgt atgctgcata tgcaccgctt gttacttgag aaaacaagat accaggaaca
    27181 tgtaactgac cctgaatgtc tgcgggaact ccacgaagcc ccgatacagg cgctaaatat
    27241 tctggttaaa cacaaactgt cctgaccgag tcatggaggc ctgcgctttt tgtgtaggtc
    27301 tcaccatcct gccctgtcat ccgtccatct aattttaagc attctgacag cctgtatatc
    27361 atttcctgcg gagttgccct gatagcgaat actgcggcag aatgcgattt atccccattg
    27421 ctgtggatat ttgagcgtga gcgctgataa ctggttagcg aggtcgttcc tgtgcgcttg
    27481 cacttaacgg gcaaccgtgg tcagggagag atgggggtta tatcgatggg ggagcatctg
    27541 gaaccctacc tggctttttt tccagttaac tgtttaccag actgaaaaac ctaattctcg
    27601 cgattcgcga taattaagtt taatcaatgg cttgtagtga ttatttaata cattgtgcgg
    27661 gggagggtta cgaaattttc gtaggccagc cttccttgtt ccggtaggga aggcatgatc
    27721 gccgtaatga tgattcagat gtgcaccttt tccaccaatg gtggtagaag ctccaactgt
    27781 aacgatgttc gttatggttg tctggatggt ctgcgcggtg attatcacca ttcagggtgc
    27841 accgcccttt gtctcgtggc ataattatca ggcgattacg tttttcatga ttgactggca
    27901 agtcttactt ctccaccgca gttctcatag ctgcggtttt ttctgcgcgt tatgccgtca
    27961 ctgaatcgcg ctcaacatac cgatccatct ccagcttcac atccagcatc atcagcatgc
    28021 cttcaacaac accttccgct ttctgtaacc gctttccgat atgtccatca gagcacccat
    28081 gcttacgtgc cagcgccata aacgttttcc ccagcacgta gtaatcaacc agcaagtcat
    28141 gcagatcgcg attgttccgg taaaggcggg ctatgcaccc gcatatcacc atcgcgtcat
    28201 cgtcgcagca ctgtggacga gatgttatct ttggcggaat catcccctta aatccggcag
    28261 caacggatga ccaggtcaca ttctcgtgat tatttgccgc ccatgccccc catcgttcca
    28321 gaacctgctg aatatcacgc atcaacaatc acctcgtaat ggctgtggtt acatacccgt
    28381 aattctgggt atgtactgga tagaagttaa cctacgaaaa tttcgtagct tgtgactgtc
    28441 tttcaggcag cttgttaaca gtcctcagaa gcgagggatg tagccagcgc aattttgcgt
    28501 ttgcggtctc ctcaaattga ggtttccgaa aatatcagta ggttaggcca aagctcaaaa
    28561 atgagctttg taacaatatc agccagttaa cgccgtcagc tacccggctc agaattgagc
    28621 cgccttttaa tcaacaagtt accgccgtaa ccgctccggc ttatgccact ggtacggggt
    28681 cgcgttctgg cgttgctcct gccgtctcct gaccaccata tgttccaccg tgtacagcgt
    28741 gcttagaaaa aaccccagat tcgtggtttt tctccggctt tttttgcgac tcgtttaaaa
    28801 ccagtcacga agtcgcttaa agatactcag ttcgacacca tcacggcaca tcagccacaa
    28861 cacgcgcata attttctgcg atcacgctgt ccggctgcgt ggtctcctgt ttacgctggc
    28921 gttctctctg ctcttcaatc atgaggatta actcgcgctc ctcctgctct tcaataatcc
    28981 cgtgaattac ctctgttggt gaacggtcgg tcattggtcc ccctgtgagt tgcggttatg
    29041 ccaggaatgt taaccagtac tctcgtttcc tgccctgtaa aaacgttcat ttttgcgggt
    29101 gacttttagt cattcacaaa atacaagggc ggaaatctgc cccttgctct ggtcagttct
    29161 ggcgtaaaaa tcgatcatca cgtatcggtt tcgttttgcg cggctgttgt gtttcgtttt
    29221 gcgcactttt tccggtggtt ttccccatgc aagccgcgtc gttactggct ttgtgctggt
    29281 ggtcagcata atcgattttg tgatcggttt tatgatcgaa tttgcgcagt tttctgcgca
    29341 cttttcgggg aattgttgtc ctacggaatg tgtgcatcag gagcgatatg taactgactt
    29401 tgtaactgac ttactgtcag ccgcaatcac tacgggtaat tgcagatttc ttacgcgatt
    29461 ttaaaatcag gccttccgtt gtgacatttc tttgatgtgc atgaggtata ttgctcgcaa
    29521 gttatctttc gcccatgtcg tcaatagttt tacggcctta attagatcgt taaacgtatc
    29581 gacatcgctc agtccgattt gcttatttgg gatttgtgtt attgatcaga tcgcataatc
    29641 tggattgagt tccctcaaat acgaagtgat ctactattcc gaacagctat ttagtcatca
    29701 gaaccgggtt tatcccggct tttttaatgt ttgatgcatt tttccatgaa agaatctaag
    29761 aattgatcgg ttattccttg aggctttcgg cgctcacaaa gagaatacaa ttcttctacg
    29821 gcagagtaag gaaaaaattt tgtgtctagc tcacgaaatt gtggtctatt tgcaaactca
    29881 attacttttt ctctatattc atctggagct acgattgtcc atcgtatatc ttgcagacgg
    29941 ggagcataat cacagaagtt cttcatacgt gctaaacccc ttattattcc ggtactatgc
    30001 tcgatctcca tcacggcagg catcaattta ccatttctaa accagatgca atctatcaat
    30061 cttgcgccag caatggcttc gctgtaggaa cttaggactt tttcattaga cagactgtca
    30121 atgacaccat ccatttgagc gattgcttta ccattgtatt ttaatcctcg gtcattagct
    30181 gcaacccatg ttctgtatcc cagatgatga ccgattttta ctaatgctat ctggatttgt
    30241 gcatgtcttc ttgattcttc aatagacatg ccttcaattg gacggattgt ttctgaatca
    30301 acactttggt atacaacttc tgtgttcatc tctgatatta caatattagt tttgtattca
    30361 gaaagcttgc cgttttcatg tggtaaattc ggtaagtata taagatgctt gtggcctttt
    30421 ttaatcgtct gtttcgtatt aataacctct aagcggtcga gtttacacca ataaaattga
    30481 ggtgtatgag caagcaaact ttcaataact gaacgagagt tatagcttgc accgaatact
    30541 ctatctaaat tgataggcgc atcctcttga attgcgttag ccaaacgcca tagcatttgg
    30601 ctagatatgc tatcaacttt ggcatctctg agcgtttcac ctttggttgg attgaaacgt
    30661 ttaaagcgta ttgggccttc tggtaactta atcgagtcga taacaatctt cccggcatta
    30721 ctttcattca cataatcaaa ctgcctgttt ttgggtagat ttgctatcgc attcacaaga
    30781 ttcattgctg ttaaattcat cttttatccc taaatgctct aatatggatt ttgctatgcc
    30841 taaagccgca aggtaaggaa caccattgcc aacagtttta aacatatttg aaagagtcat
    30901 gtaagatggc aattcgaatt ctttcggtaa agactgaatg gcaagtgctt ctgcaactgt
    30961 tattctacgt ggcttatatg gatgcaaatg tacttcatta ttaccgtaag cagctgttgg
    31021 agaatatctc cagcgatgaa gacgcttgta agattttttg ctatcgtccc cttcagctac
    31081 agctttaaag cgaactaacc cagccctagc agtaaacaca tgttttgcgt tcacatgttc
    31141 agaaactttg tttttcctaa accagtagtc aacagttaac tcttcaggaa gtgactcagg
    31201 cattgccagt gttgagttct caacaaattc attcgtagtt ggccaattat gttcaaaagc
    31261 actactatct gggtataatc ttccactcca gttaaagatc ttggttagac ttttattttg
    31321 gaaacccaac agaataatgc gttcacggtc ttgcgggact ccgtattcaa tcgaatttat
    31381 taacctttca tgaaggatgt aaccaacgtc cattaactgt tttttgagat gctcaaaaaa
    31441 ctcacggtgt ttgagcgtgc gataaagacc tttgacgttc tcgaacaaga agaagtcagg
    31501 cttcatctca acaattatat tgatgtaact ttgagacagc ttaccgttct ctccatcgcg
    31561 acctttgttc ttacctgcaa cggagaaatc agggcacggt gggccaccga tgaagccaat
    31621 tagtcttttt tctttcttga gagctttaat attatctttc aacttattgt gatcaacttc
    31681 ctcgatgctc tcttctgcat atccaaaacg tgggataggt ttgttcatca ccgctcgtga
    31741 gtgcttgtaa gcatccataa atggtgggtg aaattcattc acaaaaacca cctcgtagcc
    31801 tgagttttcg aagccaagat ctagaaaacc tgagccggaa aagaaagaga agatggcggg
    31861 tttaagtgtg gacatttttt acctatcggt gatcgttgag tcatgcaaaa acgcccctgc
    31921 atcttaccag atgaaagggg cattgcaacg ggattgcagg ctcttgtaaa ggtcgtccgt
    31981 tgtcattttt atggatatag tctttaatca acgtgttcac ctcctatgat gtgtttaagg
    32041 ttgctatgtc ttatgctgat tgctgtaagg tccatcagcc aaatacatct ctcgattgtg
    32101 agggatgtaa aaatcatgtc tggtcaggtc ttgttttcaa tgatgtgact gtctttaaga
    32161 caggtggttc acatacccag atatcgggga tgtacaaatc aggggttacg gctggtggat
    32221 ttgctcaaat caaatagagt tcaagcaatc gaataaatta gccaaaaatc aattgtttag
    32281 gtgcgagcaa cagagattat cgactttttg cattgcaata agttgaataa gcgggcaagg
    32341 ccaaagctca aaaaagagcg ctggaacaat atcagcaagt taccgccgtc agctacccgg
    32401 ctcagaattg agccgccttt taatcaacaa gttaccgccg caaccgctcc ggcgtcttcc
    32461 actggcggcc tgattgcgct atcccttcac aagcaagagc ggcgtgcccg ctgctaaaat
    32521 attacgatcg ccgataaaaa caaaagcctc cacgcgggag gctatgttga ctcagcgttg
    32581 accaagcttg ttatcgctgc ggtcacgctg gctacaatgg acgcacctta gagtagtctc
    32641 aaatgcctgt catcagatat tactgacgca ggagacaatt taagcctacc ataaaatgct
    32701 tggttttagg cattgagtgc gctggcaatt tgtagtgata gttccgtatg acatccggct
    32761 ctactgtact gcaagcaaca acagtggcca ggttagccct gcccgctata tttccagata
    32821 aaatgattac tggccttgat gatttgacca tttccggctc ttttttgcaa ctcgtttaaa
    32881 actggtcaca gagtgacttt aagttactca gttcgacacc atcacggcac atcagcctga
    32941 ttttcagcgc tcacgctgtc cggctgcgag gcctcctgtc tgcgctggcg ttctctctgc
    33001 tcttcaatca tgaggattaa atcgcgctcc tcctgctctt caataatccc gtgaattacc
    33061 tctgttggtg aacggtcggt cataggtccc tccctgtgag ttgctgttat gccaggaatg
    33121 ttaaccagta ctctcgtttc ctgccctgta aaaacgttca tttttgtagg ttactttaag
    33181 taacttacaa aaaatcgatc atcacgtatc ggtttcgttt tgcgcttcct gttgcgtttc
    33241 gtttttgaga aaaatgcgca gttttttgcg cactttttga ggtgtctatc cccctgcaag
    33301 ccgcgtcata tctagctttg ctctgtttgt ctgtgtgatc gattttgtga tcggttttat
    33361 gatcgatttg cgcacttttt tgcgcatttt ttcagggatt tttcacccca tcgaagaggc
    33421 ttctaagggg ctgtgaagtg ggtagcattt tgctgtgatg gaaggactac cgctactgtc
    33481 tgataatggt gacggaggcc gctatgttcg gctctgattg cagcctgccg tatattcacc
    33541 aatttacctg gctgttattt ccataacccg taatgtcgcc gatcccgtct gattcccata
    33601 tcgtcaacag tcttatttac agcatcctga agttcgcaag gcatatttcc cgccgtctgg
    33661 ctatatgctc tcgctgcgga tcggtgtggg cctctttcat tacgtcaagc caacgagcgg
    33721 cagctcttca ccatagtccc tgagattcca gttgttcggc ttttttatca gctattgtgt
    33781 ccatattaac ttccgtctct tcgccctgtt tcacccctgt acttcgtgcc ccgtaaagga
    33841 tcttcttttt tctggtcaat ccccatatct ttaagcgttc tggtcgctgc tgccctgacg
    33901 gcgcatacat gcccccactc tgttgttttt ggtctggtgg cttttgccgt gcattgcgcc
    33961 cttcgttggg taatatagtg cctctcttca tcagtttttg cctgtctgaa caattctccc
    34021 cagcgttcag cagcacgccg ccagaggccg ctattctcca gcgcttcagc cttgtcatca
    34081 cgtatcatct gtaccccgcc gattctgtcc tttcaaaatt tgtcttatgg cttattgtat
    34141 gagggattca tcctacaaca agaaaggtca ctatgagact gacgcaatac aaaaaagaga
    34201 tcctcagtta cttcactcct gacaatctgt cgtgggtcag aggtgaaatt ggtaacccgc
    34261 cgtttgatgt ctccggtgtg gcttacctgc tgtatggcat agattcacac aacaaccgat
    34321 atcagctcga atcaacaagg cgcacgcttg aagcaatggt gcgtgatggt cttcttgagc
    34381 ggattaatgt gtatgagcgg cgtgacagcg ttggtcatgg cgttcgtcga aaggtggcgc
    34441 gttatggcct gccaggtcgt tctgttgttg cgcgtgacgg tgagggccgg aacggtgcaa
    34501 ttgacggcga atgtatacgc atcaatgcta cagatgtggt gctggtggag gggcaataaa
    34561 aaaccctgaa taactccgct gggtgcttca attgcacggg gcgaaatcat tcagggtcag
    34621 ctgtcacggg cctctgtaca gagggctcca gttgcccaga gatgagcacc cgctacagcc
    34681 tgctgtgata agccgcttta cagtgagctt caattgctca aagaggcgcg tttttatcac
    34741 aacctgaccg cgataacccg ctgtacagag ggctccagtt gcccaaagag gcgcattctt
    34801 atcgcggcat gacgaaatca ccgtgatgtt ttcgtcgttc actcatcccg atgtgtgtga
    34861 caagtacaca tcaaaatgag tgccgaaaag ccgctggctg gtttcattcg gcgaggctcc
    34921 cgttgcctgc ggagaacacc atcaacggcc tttcggtggt gattatagcg aatcgcgata
    34981 attgagcaaa tacggcgatg agatgcgtat cacgatagcg ctaaatcgtc gcctgcatca
    35041 ctgaagctgt aatttttcca ttcatcctgc ggattaatcc ccatctcttt cagcgttctg
    35101 tctgcggcct ttttcacatc ggcaaaggtg acgaactgct caggcatacg acgggattta
    35161 atgatacata tcgcccgacg tctggcagct tcctcgcgtt ccctgtccgt ttctgcgtga
    35221 agcaggacgt cagaccatcg ggtcgccgca cgtcgccata aaccgcgctc ctcaagtttc
    35281 tgtgccacgc tgtccgctat cactggcttt acctccggct gattactgct gactgttttt
    35341 ctgcttcatc tgctccagtc tccgcttcag tagttcgctg gctactgatt tatcttcttc
    35401 cgtgacttca ccgcaaggtt gcccgttcag gtcataccgt gcgccgccag cgacaatggc
    35461 tttcaggtat cgcgtcgtgt ggatgtatga ggttaatgcg ccacgtaata cgccatgacc
    35521 aatcgccagt tcccgcgcct tcgcgtcctg caacatatcg tgtatcacgc cagccttaag
    35581 gggttttata ttctctgccc caaacagttc aggtcagtac tcagccagtc ggtttatcct
    35641 cctgcgtttt tttcgctgcc gctttgtcat gtgctgccac ggtatgcgcc gtgatggttt
    35701 acgtgtggtc tggtcctgtt gtgtggtctg ctggttcatg ttgtgctcct gtcattcaga
    35761 acggaatatc gtcagagaac gggtcatcac ctcccgctgg tggctgattc ccccgtgttc
    35821 cggtctgtcg ttttgctctt tccagtgctt cggtcgcctt cccctgttgc ccccgcttcc
    35881 cgccaggacg cgccgaacgg gcactgatta cgctgtcagc gataacctgc cagccctgcc
    35941 gtgtttctcc gttctgtccg gtccactgac tgatctgcat gttacccgcc acgctgataa
    36001 ggtcgccttt ttcatgtctc gccagcgcgt cggcctgctt accaaatgcc aggactgata
    36061 gccataacgt cgcctgtccg tcctgtgcct gactgcatgg caaagatacc gccatacgcg
    36121 tcagcgtcat cggtgtgccc ttgctggtct gtttttcctg cgggtcgtcc accagccgcc
    36181 cgtaagctgc aatctgtgct gtcatggtct gcgctccttg ttccggtact cctggataat
    36241 acccattgtc atatcgaata aatcacatgc cgctttttct gcatcatccg ctttcaggtc
    36301 atacagcgtc atgaaatggt aagtgatcgc ccgtgcggct cttttgctgt ttgctgcata
    36361 tttctcatag atttttcctg tccgctcttc aatatcccgc cggaactctt cacgttcaaa
    36421 caggtcacgg tgtatcaaca tcagcgaatc attcagccat gtcacatcgg cgtataccgt
    36481 tcgcttccag acaatcagga tcggaagttt atgtttgtcg cagtgatgca gatgttcgtc
    36541 aggtatataa agcgtggctc tgcctcgtac cgatacgaac ctcacaacag attccggttc
    36601 ggttaaatcg atagcgtgtg aatgtgattt tttggcgtgt ttctctgtca taaaattttt
    36661 ctcagaggaa taaataaaaa tttatggcaa aaaactgttc aactgttcac cttttgctat
    36721 ttcgccttta gattcatgaa gttaaggggt gaacactttt tgtaaaggtg ttcaccaact
    36781 gttcacccgt gactgttcga aaatcataca aaaaaggtga acaggtgaat actttggtga
    36841 atagttttac aaaactgttc accccttaac atattgatac taaatacatt tattgcaggg
    36901 tgaacagtgg tgaacagttt ttctataact taatttttat caccctcatt tttcactggt
    36961 gcacatgacg gcagccagtc ctcagactca tccgtcagta tcacgtttga tcgcacacct
    37021 tctttagttc tctttttcat atactccctg ccatattccg ccattgcgcc tggcatatct
    37081 gcaccgaagc gcgtcagtgt tacgggctta ctgaaaccgt gcgccctcat atacgagaga
    37141 taggcgtgat aaagataacg gcgtggactg aaaggaataa tctcagcatt accgactaac
    37201 aggccgtcac acattaccga cgacatgaga tagccgcaga agtccaccag cgaatccccc
    37261 tcacgcttta tcgccagtgc ttcttctgat ttctgctgct catataaaag gcgtctggct
    37321 tcgtcctgat cagcaaaccg tgtaagcaga tgacgaatca ccacagcaag ctcaccttct
    37381 attttttccg gcagcatcgg atcgcgttcg ttttccggta caatctccga aaaattgaat
    37441 atcacacgac gacgtgagat ccccccgctg cggtcactga atgacatggc gctgttgtta
    37501 accgccagca caaccgccgg aatacgcgtt gagtatggcg ctttgtgttt cgggtcaatt
    37561 gccaccttgt caccgcccgt aatggccttt atccctgcac catcaccagc gtagcgggtc
    37621 atatccggca gaataatcag tgaaaagcca accactaatg cgcgttccct tgcatcctcc
    37681 agtgccttca tgcttgccga taccgtgttg gccttacctg ccagcatggt acaaatctcc
    37741 gccataacac ttttaccgct tccaccagga cctgttacct caatgaataa ctgccagtcg
    37801 taccggttcg ccagcaccat aaataacgct gccagaacgc gatctgcctt gcggtcattc
    37861 tcagccaccg aacggcgtaa ccacttccag aaattcggcg catgtgttgc cagcgtttca
    37921 ccctctgctg gtggggtgaa cggtaattca ctggcaatta acagccagtc atttttatcg
    37981 tgttcacgga aatcacctgt acgggtatca aataccccgt tactgaatcc aatcaggtta
    38041 cgggcggtat tccccattac ggataaactt aacttcatgg tatcaaccgc cgattttatg
    38101 gcgttttgtg aatagcagac atcttcgtca atgaagattt gcgccattgt gcgctgtagc
    38161 tctttatcct gtacgggaac ccatacaaca ctgttgtaat aatgcactgt gtcagagtcg
    38221 gcgtttatta ccagttcgcc gccataatgt gccagaagaa cttcgccgcg ctggcttgcc
    38281 cccatctggt ttagcgccag tgatgtggca tcatcacctt ttccccgctc ttttttcttt
    38341 acaggcagct caataaccag cccgtcagaa atgtttttgc gctcagtctc cagatagtta
    38401 cgccagttct gccgctcgtt atggtgcatt ccttcggggt agtaatcagc atcctgtacg
    38461 cctgccgccg ccagcttctg acctatgcct ttgaccatca cctgatcgag gtgtccggcc
    38521 ttgtatatcc gcgctgattt gcgcccgtcg ggtacaattt gcagattgcc aatatcttta
    38581 agttgctcat cagccagcac gacaggcggt atattatctc cggcaagttt tccgtcctgc
    38641 tccagccact gtttcgcgtg cgcccacgca tcactgccag cgaaaataat cacttccgta
    38701 tcggcgtgtt ttttccctcg aggctgtttt tttaagttcg gtgcgctttt catttcttac
    38761 ctctgctgat catcgtcatg tggctaccgt attcattgcg gtaaatatcc ccgcacataa
    38821 attcggggtg agttttgcta ctggcagtta agccagaata tttacgtttc atcgttttat
    38881 ttcctgatat gtaacgcttt aattcgctgg tatttatttc atcctgttac ttcttccctt
    38941 attgcatatc acccgatgca gcaatcagcc agctacctgc cttacctgta agctgcttaa
    39001 tcagggcgag caatgactca atctcaccct cagtcatttc acctgtgtta cccatcatca
    39061 gagaacaaat aagttctgcc tgcattgcgc tttttgctgc ttcatcaaca gtcatatcga
    39121 aaagatcact catggattac tccttatcct gaatacatgt aacggcgtgg ataattacgc
    39181 cggataaacc ggctttagct gatttgttaa ttactgtgct aattgcggcc tggacgtttt
    39241 cggccttcac gttctgcgtg atgccaacgg tatagccgcg tgcattaacg gcacgggaaa
    39301 agacgcggaa ggttttaagc atgagtcacc tcctgacgga tacgggcggc gaatacagca
    39361 acgcaacctg acgggcaacg gttacgcgct tcgcgttccg tccaggcggt tacgttgatg
    39421 atttgggatt ctgacgcgcc aaagatgata aagcgccaga taaaggcagt ttgagaatgt
    39481 acgagggcag ggatagtagc catgttggca gcctccttac agtgatgaat aacgtcaccg
    39541 caggagttct cacgctcaca cagggcggtg acactggcgg gggtgagaaa accggactgt
    39601 aaggactccg gcctaccttt cggtagcccc gccagcatca ccattgtttg ctggtggcgc
    39661 tgtgcaaaat gcaactcgtg aaatgactgg atggtgcacg agtacgcaca atacacaatg
    39721 ccattgatat ggcgtaaagc gctggcagaa aaaaagacgc ttctggcgtc atttatcgcc
    39781 ttacagttaa acgggttctc acgcccgatc cccgttttat aagggatagc ctcagtatat
    39841 gccgcttttt tgcttaagtg gtcggatgtg ttgcgagaga agtttgtgcg aaaaatattc
    39901 acatatcctt ccagaaagga tggataccag aaagaaagac aacgtaccag aaaataacca
    39961 gcgccgtgat atgaggcatc acgacataat gcattaccgg aatccagtat gagtaagcac
    40021 agattttcgc ccgttacccc gaatatcagc gcactacaac gccagcgact gatacgtgac
    40081 atcattgaaa ccaggcaaga aaaattaacc ccgccagaaa aaaccagcga accgccaccg
    40141 cttgaggtgt ttctcagtgg cagaaaaaac caacgcaaca aattacaaca acagaaacca
    40201 tcatcgctgt agcgtggtgt gttagggcgc attgctttac agggcagcag catgaacaaa
    40261 aagtaccaat ccattacaga tatttttgcc gaacaacgaa gacagctttt gcaccgcata
    40321 cttgagcgcc aactggaact gaaacgcgct aaagagatat cagaacccga agagttaacg
    40381 cctcttcccg gagtgaaaaa acacccacaa aaacgatttg cccttgatga ctttattgag
    40441 aaatacccac ggcggttaaa agccactaaa aaccgaccgc caggctgata gctcgctgtt
    40501 tcttctgcgg ctctatgaaa cagacagaaa ccatcagcgc cgtgatgtga ggaagtacgg
    40561 cgcattgttt tacaggtgca caatatggat aacacacaac acacttcaca atcagagaat
    40621 aaaaaagatg atctcacagc atatgagcaa catttcgttg aaaaaaaaca gtcaataacg
    40681 agacacatcg cagatatgga tatgcgcaag cgatttgaaa aaactttcaa taccaatttt
    40741 gcgcttgagg attttttaaa gcagcattcc agccgatcaa gcaataaggg atagcttatg
    40801 gatgaccaga aaaaaaactc ctttgccatg atggttacac tgaccagcgc ccgtataact
    40861 gaacatgaac gaaagcggaa agctgcacgc gaacaggcaa gagcaacaga attaacacgt
    40921 aaagaactta acgctctggc acgggaacac agagaagcac ctgaagcttt tgcacttgat
    40981 aattttctga aaagtacatg ccgtaagaaa atacgaacat cgggaccaag gggccattcg
    41041 tcgtaaactg catattgtcc gccagcctca gatatggcat tgtggataat gcgcttgatt
    41101 tcctttatct ccactggcga ccctttcgtg gtcgcctttg ttttgcctgc cattcctgca
    41161 tcatgcggat attcgcgccg tacagggcca ccagcgcgat aatttgataa aacctccctc
    41221 aagtattgct taacgtgttt catgcgctca gcggcgaaat ggctcacgat atggcgcatg
    41281 taatccgcat ggttgatgtt cagtagtgcg aacttatccg ccgtgtcgat gtaccggaca
    41341 gcaggtttga ggatgtcagg catgttcatg ctctcccatg cgacgggcca gccagcgctg
    41401 tgaaaggcgg attaattcgg cttttcgctg ctggtaagtc atacccaact caatcagcgt
    41461 gatattgctc tgctcaaggt aggaaaggtg ttcaagctgc ataacgctca tgctgtcacg
    41521 cggttttcct ttgatgccgt tcgcctgcgc ccattgtttc gccgtcatgc cgcccagcac
    41581 gatacgcgcc agcatatttg attctgtggt gtagtggtgt tgatgagtct cttttcccag
    41641 ctctacgcgg tacaactcca gcgcagcgca cataggctta aaaaaactgg caacggtgac
    41701 acgcgctttc agttcacggc gcaaagctgc ggcacgttcc ggcgctgcct tgtgtagctc
    41761 ttcttcacat ttgatgaagt actggcgaat tgcgcggccc tgttcagtgc gttcgaccat
    41821 cgccagttct ttacccatgt tcacggtgag caagtaatca tgagcaagct gctggcgaga
    41881 ttttgcgctc gccggatttg gcgagctcaa attttctaca acctcaaaat tcattccctt
    41941 tttaaattca tactcctcaa tacgcccttt aatccaattg gtgaaatcac ggcctacacc
    42001 gagcgcttta tgcagtgcgc gtgcgctgac aatgcccgtt tcatatccgc caataatgcc
    42061 aggtgatacc ggaataattg cggcgaaata ctgttgactc agttcttcag gctgaacgaa
    42121 gccctgcccc tgtacgggca tattcggacg tttcattaaa aactcctgct gtagaatcag
    42181 gtttggtgct gacgggcgcg gaactgtacg cccgtcatgc tgattactgc ttggtgttgt
    42241 tgcggcgctc gttcagccag tggttgatct ccaccgcatt aaatacggtg tttttctccc
    42301 ccagcttcgt gggcttcggt aatttgccga tacgcaccca gcggtcgata gttgacacgc
    42361 tggtatcaag caacttagcc aggttgcagc ggcggatata gcccgtggta gggatagctt
    42421 cttgatggtt agctgtcatg attccctctt gggttaaaag ttgatgtttt ctccattcta
    42481 ttggttgttt gtacagcatt ccatcaaatc tggaatctat taaaatattc acaaattcta
    42541 ttgaaataat atgtttccag taaaagattg ggtgagcggg aagaggtgct aagactttga
    42601 aagtttttgc tttattggcg ataagtcact gaatccgtat acatcctgat accgtgatat
    42661 acccactgcg tcgttatgcg ctgttatggt ttgccatctt ttgccagtct ttaacgtgct
    42721 aatcctgttt atatcgattt ataacgatta ataacggtga gtaaattttg ctggtggggg
    42781 atgtatgggg attacagccc cgtgaataca gggcgtgttg gctggttaat ctgcctggct
    42841 tactgccagt aatgcggctg atattttttg cataaacgtg gatttggcga tcccttttgg
    42901 tgatatccct ttctccttca tcaacgcttc cacgtcgcgg taaattgctg aacggtttgg
    42961 ctctccgtcc tgaatgtatg cggttgtgcc tgattttttc acgaggagta aaaccagtaa
    43021 ccccatcatc ttcatgtttc cctgctcttc gtcgagtttc ctgtgctgcc cccttccgct
    43081 gcggcttgtt tctttgatgt aacggaccag atcccgaccg ccaagattaa gggctttttc
    43141 ctcccagttt tttgtcccac gcaaatcatc gatagtatct gttaccgcct tgattattgc
    43201 ctctggcgtg cctgcctcca tgaacgggaa ggaagcaagg aatatatccc ccgctctgta
    43261 tggctcatcc ttgcttgttt ttactccact atgcagcctg atagcacgtg cgatatgagt
    43321 tctggctgat tcgacctgtt cgaacttgtc ctgcggtacg tcgcctatcc tcattgacgg
    43381 attaagatca gctaacgcaa gggccatttc gtggggagtg attgaaacag aagcgcaaag
    43441 gcgctctatt ggcgatttct tcaggatgtt aatcatgatt tagcccttct gattggtgtc
    43501 acgttgtagt gttctccgcg ttccagcgac actaacagat tggtccactt cgccagtgcg
    43561 tcccgtcgtt cattgaaata ctgatagcgg ttgtatatgc cttcgactcc cttgatgcgg
    43621 tgattgaggc atctttcagc gactaccgga tcaacaccaa gcgcagctag atgtgtgcgt
    43681 gctgtacggc ggaagtcgtg aatagtgaaa tttggaactt ccggcatctc tgcgcggact
    43741 tttgccagcg caacaggtag ggtgctctcc tgaatgtggg ggatcatcct gtgctgcatt
    43801 ttccgcgccg gaagcaccca ggcactattg catgacaaca cacgcaactc tcgcagccat
    43861 tcaataacgg gatggacgag tggaatatcg atggggtcgc cgtttttgct gcgttcctcc
    43921 ggcagatgcc atacagcgtt atcaaggtcg aattcttccc atctggcggc gcacaactcc
    43981 atcttcctga cacaaagcgc cagcaatagc ttaaatgtga gttcattctc gcggctgaaa
    44041 ccctttgcgg tccgcatagc ctgaaagaat cggattagct cttcacgagt cagccaacgg
    44101 tcgcgggagg actccttgcc accagcatca gagacttcaa acgcagagca tgggttaatc
    44161 tccagcacat gacgttttac cccgtagtcg aatatgcgac gcgtccagcg cagaacgtcc
    44221 gttgctatgg ttggcgctcc acggtcaaca attgctttta gcatctcgtc aacgtgtcgt
    44281 ggttttacat cctcaacctt catatgacca atacgcgggt ttatgtcctt gtcgatgcga
    44341 cgacggagga tatcgggatg tttccagcgc ggcaggatct ggcgttcgaa atattcggcg
    44401 gcaagctctg atactcgtat ggcgtttttt tctgcctcca tttttgccag tgcttcggct
    44461 ttacgctcct gtttctccgc tgctacgtcg tacccaagtg cgacgcgggc ggatagttcc
    44521 tttgctgttt ctctggctct ggatagtgat aactctgcat atgacccgat aaccatcgcc
    44581 cgttgtttgc cagcaaactt atagcggaat cgccagaagg gcattgtgta atttttggga
    44641 tagcagagat acaggccgtt accgtcagct ttgccgtcaa acctctcgtc tgcctttatc
    44701 caggcacgga tctgaatatc tgtaagtttt ggcattaagg catccctaaa cattgaatag
    44761 aggtgtgtac ataaagcgat gtacataaat ggcattatgt acacctttat gtacacgcta
    44821 atcatgcgct ttcttgcgtc gttatgatag tcattaatta caaaaaaaac ccgcattttc
    44881 gcgggtttga ttggattatt gcgctttatt gatttcctct gatagcttac tccacatttt
    44941 gaatctgctc gcgcatctgc tcaatcaaca ctttcagctc aatcgcggag tttgtcactt
    45001 cggcattgat agacttcgaa gcaagagtgt tcgactcgcg gttgaactcc tgcatcataa
    45061 agtccagacg acgaccaacg gcttcttttt tcttcagaat gttgtacgtc tctttaacat
    45121 gcgcttcgag gcggtccagt tcttcggcaa cgtcaattcg ttgtgccagc agaaccaact
    45181 cctgctccag acggttgttt tccaactgca cctgagcatc ttccagcttc gcgaccaagc
    45241 gttcacgctg ccattgcagg atttccggca tatgggagcg gactttgacc acttcggcgg
    45301 tgacgccttc cagacgctgc tcaatcaacg ctttcagcgc ctgaccttcg gtttcgcgag
    45361 cgatgataaa gtcatccagg gtgccatcca acgccgcgag aatttcagcg gcaatcgcgt
    45421 cgagatcctg ctcctgggcc gccatcacgc ccggccaacg gagaatatca actgggttga
    45481 tttctccttc gtcgctctgc atttttaccc agttcgcggc agttaccagc tgtttagcca
    45541 gtttttcgtt gaggatcagc tcaccttgcg cgctaacatc tggctcatag cgcagggtac
    45601 attccacttt accgcgtgtc aggcgagaac gaatacgctc acgaacgaca ggttcaaggc
    45661 tacggaactg ctccggcaga cgaaagtaag tttccagata acgctggttt accgagcgca
    45721 tttcccaggt tgcgctcccc cattcaccct tgatttcacg ccgggcgtag gcggtcatac
    45781 tgcggatcat agacgttcct gtttataaaa ggagaggtgg aaggattata gccatcgatg
    45841 ccttgtcagg ataggaataa ccgccggaag tccgtataat gcgcagccac atttgtttca
    45901 agccggagat ttcaatatgc gtccagcagg ccgtagcaat aatcaggtgc gtcccgttac
    45961 cctgactcgt aactatacaa aacatgcaga aggctcggtg ctggtcgaat ttggcgatac
    46021 caaagtgttg tgtaccgcct ctattgaaga aggcgtgccg cgcttcctga aaggtcaggg
    46081 ccagggctgg atcaccgcag agtacggcat gctgccacgt tctacccaca cccgtaacgc
    46141 tcgtgaagcg gcgaaaggaa agcagggtgg acgtacaatg gaaatccagc gtctgatcgc
    46201 ccgtgcgctt cgcgcggcag tagatttgaa agcgctgggt gagttcacca ttacgctgga
    46261 ctgcgacgtg cttcaggctg atggtggcac gcgtaccgct tcgattacgg gtgcctgcgt
    46321 ggcgctggca gatgcgttac agaagctggt ggaaaacggc aagctgaaaa ccaatccaat
    46381 gaaagggatg gtagccgcgg tttctgtcgg tattgttaac ggcgaagcaa tttgcgatct
    46441 ggaatacgtt gaagactcag ccgcagagac cgacatgaac gtagtgatga ccgaagacgg
    46501 gcgcatcatt gaagtgcagg ggacggcaga aggcgagccg ttcacccatg aagagttact
    46561 caccttgttg gcgctggccc gagggggaat cgaatccatt gtagcgacgc agaaggcggc
    46621 gctggcaaac tgatttttaa ggcgactgat gagtcgcctt ttttttgtct gtagaaaagt
    46681 aagatgagga gcgaaggcat gaaaccatat cagcgccagt ttattgaatt tgcgcttagc
    46741 aagcaggtgt taaagtttgg tgagtttacg ctgaaatccg ggcgcaaaag cccctatttc
    46801 ttcaacgccg ggctgtttaa taccgggcgc gatctggcac tgttaggccg tttttacgct
    46861 gaagcattgg tggattccgg cattgagttc gatctgctgt ttggccctgc ttacaaaggg
    46921 atcccgattg ctaccacaac cgccgtggcg ctggcggagc atcatgacct ggacctgccg
    46981 tactgcttta accgcaaaga ggcaaaagac cacggtgaag gcggcaatct ggtcggtagc
    47041 gcgttacaag gacgcgtaat gctggtggat gatgtgatca ccgccggaac ggcgattcgc
    47101 gagtcgatgg aaattattca ggccaatggc gcgacgcttg ctggcgtatt gatttcgctt
    47161 gatcgtcagg aacgcgggcg cggcgagatt tcggcgattc aggaagttga gcgtgattac
    47221 aactgcaaag tgatctctat catcaccctg aaagatctga ttgcctacct ggaagagaag
    47281 ccggaaatgg cggaacatct ggcggcggtt aaggcctatc gcgaagagtt cggcgtttaa
    47341 agaaactcgc cggatgaaaa gtcatccggc gtcatattac tgcaactgcg cggcaattag
    47401 cggccagcgg gcgtcaaaat catccgtcgg gcgatattta aattcgctgc ggacaaaacg
    47461 tgacagcata ccttcacaga aggccagcaa ctggcttgcc agcagcgttt catcagtggt
    47521 gtaaccttca ccctcacgca ttctcttttc acgcaatacc tgacgcagct gcgcttcaat
    47581 acgctcgaaa agttggttga tgcgcccttg caggcggtct tgttcaaaca tcagcgcatg
    47641 cccggtaagg atgcgggtca ggccaggatt acgctcacca aaaccgagaa gcagcaacac
    47701 tatcagacgc aggcgcgctg tggtgtcttt ctcatctttc agaatcagat tgatgcgagt
    47761 aatcaggcta tcttcgataa actcaatcag gctatcgaac atgcgggtct tactggggaa
    47821 gtggcgatac agtgccgctt cggaaacgcc gacagaggcg gccagttttg ccgtcgtgat
    47881 acgttggctt ccatcgctgg attccagcat cagcgccaga gactgaagta tttcctcgcg
    47941 acggttcctt ttcgcagttt gtttttctgc catgttacaa aatacccctg aaaataagca
    48001 cttgccaggc ggcacccacg ctatgaccgc aaacgaaatg tttgcggcta tgttatgacg
    48061 ttattcggat gcgtatgtgt tactgacgac cagagtgacc aaagccgcct tcaccgcggt
    48121 cggtggcgtc gaaatcttcc accagattaa attcagcctg tactaccgga acaaaaatca
    48181 tctgggcgat gcgttcgcca ggttgaatgg tgaagctgtc ctgaccacgg ttccacacgg
    48241 aaatcatcaa ctggccctga tagtcggaat cgatcaatcc taccaggtta ccaagcacga
    48301 taccgtgctt atgtcccaat ccggagcgcg gcagcatcat tgccgccagt gaaggatcgg
    48361 caatatgaat tgccagcccg gtcggaacca gcgtagtgtc acccggagcc agttctacgg
    48421 cgtcgtcgag acaggcacgc aggtcaagtc cggcagagcc agaggtggca taagtcggga
    48481 gcggaaattc cttcccaacg cgcgggtcca gaatcttaac gtcgattttt ttcatcataa
    48541 cgggtcacga tctcgtcgag taataattgg ccaaggagct ctttgcgctc aagcggtaag
    48601 actttatctc cgtcctgcca gaaaaggtgt aatgcgttgt tgtcgctgtt aaatccttga
    48661 gttggctggg aaacatcgtt cgcgcagatc agatcaaggt ttttacggat acgtttttgc
    48721 cgggcgtatt cttccacatt atttgtttcg gcggcaaatc caacgacgta gggtcgatgg
    48781 tcttttagtg cggcaacgcc tgcaacgata tcggggtttt taaccatttt tattgttaat
    48841 tcatcaccct gcgtggcctg ctttttgatt ttttctgggg ccacggtagc tgcgcgataa
    48901 tccgccacgg cggcgcagcc gataaaaata ttttgctgct gtacagaagc attcacggcg
    48961 gcttccattt ccagcgcggt catcacatca acacgtttaa caaacggtgg cgtcggtagt
    49021 gaaactggac ctgataccag cgtgacgttc gcgccacgac gggcagcggc ggcggcgata
    49081 gcaaaaccca tcttgccgga gctgtgatta gagatataac gcaccggatc gagcggttca
    49141 cgcgtcgggc cggcggtaat cataatgttc agatgtttca ggtcgttgac gggcgaaaaa
    49201 tgcgctactg ccatatccac aatggttaac ggatcgagca ttcgcccagg accgatatca
    49261 ccacaagcct gactgccact gtctggcccc cagatgagca aaccacggga agcaagcacc
    49321 tctaaattat gctgcgtggc agcggcacgg tacatctgct ggttcatggc ggggagcacg
    49381 gctacaggcg caggtgtagc cagacaaatc gtcgatacca ggtcattcgc cattccggca
    49441 gcaacacggg caatcaaatc tgccgtggcg ggggcgagaa tcactaaatc agcccattta
    49501 cccagctcaa tatggcccat agcggcttcg gctgccgggt caagcagact gtcggaaacg
    49561 ggatacccag aaaccgcctg caagctaagt ggggtgataa aggcttttgc cgcttcggtc
    49621 atggctacgc ggacgtcggc cccgcgatcg cgcaaacgac gcaccagttc aggggtttta
    49681 taggcagcaa taccgccgct aacgccgaga acgatttttt taccggccag gctcatgatg
    49741 atttttcctg tagggtgaca ccagaagttg gcgattttat cacaatactt ttgttgtcgt
    49801 gccttcacct gagattcact ttgcgaggcg ctttccagga ttgaaaactg accgtcgatt
    49861 taacggaacg gctatgacag gatgcgagca ccacaaagga ggtgaaggtg aaaaacaatg
    49921 cacagctgtt gatgccgcgc gaaaaaatgt tgaagtttgg tattagcgcc ttaacggatg
    49981 tcgagctgct ggcgctattt ctgcgtaccg gaacgcgcgg taaagatgta ttaaccctgg
    50041 caaaagagat gctggagaat ttcggctctc tttatggctt gttaacctct gaatatgaac
    50101 aatttagtgg tgttcatgga attggcgtag cgaaatttgc ccagttaaag gggattgctg
    50161 aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga aagcccttta ctcagcccgg
    50221 agatgacgcg ggaattttta caaagccagc tcacgggtga ggagcgggag atctttatgg
    50281 tgatctttct cgactcacaa caccgggtta taacgcatag ccgtcttttt tccggcacgc
    50341 taaaccatgt tgaagtccat cctcgggaaa ttatccgcga agcgataaaa ataaacgcct
    50401 cggcgctgat ccttgcacat aatcaccctt cgggttgtgc tgaacccagt aaagcggata
    50461 aactcattac tgaacggata ataaagagtt gtcagttcat ggatttacgc gtgctcgacc
    50521 atatcgtgat tgggcgtgga gagtatgttt cttttgccga acgcggctgg atttaacccg
    50581 ctatgcgcga tccttcggga tctttgtctg ttcgggactt gagcacatcg ctgagtcagc
    50641 gtatactacg ccacctttga gaatctcggg tttggcattt gggcctggca atcgagagtt
    50701 cacatagaac tgcgatgacc gggctgtaaa gcctgacgag gcgccaatac cccatacgaa
    50761 gctcgagcta atttgatttt tggagaatag acatgtcccg agtctgccaa gttactggca
    50821 agcgtccggt gaccggtaac aaccgttccc acgcactgaa cgcgactaaa cgccgtttcc
    50881 tgccgaacct gcactctcac cgtttctggg ttgagagcga gaagcgtttt gtcaccctgc
    50941 gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg catcgataca gttctggctg
    51001 aactgcgtgc ccgtggcgaa aagtactaag tacttagagg aaataaatca tggctaaagg
    51061 tattcgtgag aaaatcaagc tggtttcttc tgctggtact ggtcacttct ataccactac
    51121 gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa aaattcgatc cagttgttcg
    51181 ccagcacgtg atctacaaag aagcgaaaat caaataattc tcgctttgat gtaacgaaaa
    51241 acctcgctcc ggcggggttt tttgttatct gcttaccccc atattgattg catctgttca
    51301 ttcctggaga tgctatgcct gaattacccg aagttgaaac cagccgccgc ggcatagaac
    51361 cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg caacggacgc ttgcgctggc
    51421 cggtttcaga agagatctac cgtttaagcg accaaccagt gcttagcgtg cagcggcggg
    51481 ctaaatatct gctgctggag ctgcctgagg gctggattat cattcatttg gggatgtctg
    51541 gcagcctgcg catccttcca gaagaacttc cccctgaaaa gcatgaccat gtggatttgg
    51601 tgatgagcaa cggtaaagtg ctgcgctaca ccgatccgcg ccgctttggt gcctggctgt
    51661 ggaccaaaga gctggaaggg cacaacgtgc tggctcatct tgggccggag ccgcttagcg
    51721 acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa gaaaaaaacg gcgattaaac
    51781 cgtggctgat ggataacaag ctggtggtag gcgtagggaa tatctatgcc agcgaatctc
    51841 tgtttgcggc ggggatccat ccggatcggc tggcgtcatc actgtcgctg gcggagtgcg
    51901 aattgctggc tcgggtgatt aaagcggtgt tgctgcgttc gattgagcag ggcggtacaa
    51961 cgctgaaaga ttttctgcaa agtgatggta aaccgggcta tttcgcgcag gaattgcagg
    52021 tttacgggcg aaaaggcgag ccctgtcggg tgtgcggtac gccgattgtg gcgactaaac
    52081 atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa gtaattcatg cgcgccggat
    52141 ggcataccat ccggcataaa cgctacgcta acttcgccat cagcgcctga tggacattct
    52201 ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac ctctttcacc aacgatgaag
    52261 agataaacga ccactctttc gacggcatca gaaacacact ttccagttcc ggcattaagt
    52321 ggcgattcat atgcgccagc tgcatttcat attcaaaatc cgccaccgca cgcaggccac
    52381 gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc catcaaatca ctaaacccga
    52441 ccacttccac gttccccaga tgcgcggttg cctgctgtgc cagctcaaca cgctcttcca
    52501 gggtaaacat cggtttttta ctggggctgg cggcaatcgc cagaataacg tgatcgaaca
    52561 tctgcgtggc gcgcgtcacg atatcgatat gaccattggt aatgggatcg aaagtacccg
    52621 gataaatcgc ccgtttttgc ataacaacct caatgcgttt tcggtggcag gtaaggttcc
    52681 agcagttgaa gcagacgctg tagcgcgccc tggttttgat acagtacttc aacggcatga
    52741 cggccataga aactacggta atcggcgtcg gtgagtaaag aggaaacctc ttttgcaagc
    52801 gtagtggcat cggtaacggt aatcagccca cttgcctgct ccagccgcgc gcaaatgtct
    52861 ttaaagttaa aagtatgcgg ccccatcaat accggaattg cgtgtgcggc agcttccagc
    52921 ggattatgcc caccacgttc aaccagtgaa ccgccaacaa aggcgagatc ggcaatgccg
    52981 tacagtaaca tcagctcgcc catcgtatcg ccaaccacaa cctgcgtgct ggaggaaggg
    53041 acttcccctg aagagcgtgt gatatagctt agtccagcct ggcggacaag gttaatcgca
    53101 tccgggaagc gttccggatg acggggtacc aggatgagca gtaaattcgg gaattgctgt
    53161 aacaatgcct gatgtgcggc gatcaccaca ctctcttcgc cttcgtgagt gctggtggca
    53221 atccataccg ggcggtgtgg tgcccactgg cggcgcagcg tcacggcttt agcagccaac
    53281 tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa cggttacttg gttatttttt
    53341 gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt gggcggcaat cagcgtaata
    53401 cgacgcagca agcgacggac gaatttaccc agtttggcat aacctgcggc cgagcgggca
    53461 gagagtcgcg cgttagcgat caccagcgga attttacgtt tatgtagcgc cgcaatcagg
    53521 ttaggccata gttcggtttc cataatcaac accagtttag ggtcgacttt attcaggaaa
    53581 cggttgagtg catcgggcag atcatacggc agataaacgt gctgaacatc cttcccgaaa
    53641 gccgattgta cgcgctccga accggttggc gtcatggttg taacggtaat cggtaaatca
    53701 ggataacgat gacgcagcgc gcgcaccaac gggatcgccg ccagagtttc accgacggag
    53761 acggagtgca gcataatgcc gcctggtttt agcggatggc ggtaaaaacc gtaacgttca
    53821 ccccagcgtt ttcgataggc cggagcctta cgtccgcgca cccagagccg tatccagatc
    53881 agcggctgaa taaggtagag aagggcggtg taaagcaatt cgagcatagt aaatagctga
    53941 cttatggatg tgctggggat tctatgtatt tagctgtggc tttaccatta cttttcccgt
    54001 ttttgactta aatagcaatt tacccgccaa ttcttgctga acatctatac tttttttgta
    54061 tttttatgcg attcattgat ttctgctgta cgtgcaggaa ggaatcgcag agcaacagtg
    54121 aaagaatatg gttgctaatg attttatttg attgatttct ttattgaaaa tcaacaatgt
    54181 gcttaaaaat cattcgggtc ggattgccgc gaaagaagtg atacactagc gcgtcaatat
    54241 cagtgcggta tcagtattca ggtagctgtt gagcctgggg cggtagcgtg ctttttctgc
    54301 ttcaccttaa tcggataatc tcaacaaaaa gagttacttg tggataagcc atttcgaaga
    54361 attttgctca ttaagatgcg ttttcatggg gatatgttat taactactcc cgtcattagt
    54421 tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc tgctttatca ggacaccatc
    54481 ccgatcctgt ctgaaaatcc agagattaac gcgctctacg gcataaaaaa taaaaaagca
    54541 aaagcctcag aaaaaattgc caactttttt catctcatca aggtattacg tgccaataag
    54601 tatgacctta tcgtcaatct taccgatcaa tggatggttg ctatactggt tcgcttatta
    54661 aatgcccgtg tgaaaatttc ccaggattat catcatcggc agtctgcttt ttggcgtaaa
    54721 agtttcaccc atttggtgcc gttgcagggt ggaaatgtgg tggaaagtaa cttatccgtg
    54781 ctgacatcat tgggacttga ttcgttggtg aagcagacaa ccatgagtta cccgcctgca
    54841 agctggaaac gtatgcgtcg cgaacttgat cacgctggtg ttggacaaaa ttatgtggtt
    54901 atccaaccta cggcgcggca aatcttcaaa tgctgggaca acgccaagtt ttccgctgtg
    54961 attgatgcct tacatgctcg tggttatgaa gtcgttctga cgtccggccc agataaagac
    55021 gatctggcct gcgtcaatga aattgcgcag ggatgccaga cgccaccagt aacggcgctg
    55081 gctggaaagg tgaccttccc ggaacttggt gcgttaatcg atcatgcgca gctgtttatt
    55141 ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta atacgccgct gatatcgctg
    55201 tttggtgcga cagaccatat tttctggcgt ccctggtcaa ataacatgat tcaattctgg
    55261 gcgggagatt accgggaaat gccaacgcgc gatcagcgtg accgaaatga gatgtatctt
    55321 tcggttattc cggcggcaga tgtcattgct gctgtcgata aattattgcc ctcctccacg
    55381 acaggtacgt cgttatgatc gttgcttttt gtttatataa atattttccc tttggcggtt
    55441 tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc ccgaggtcat catgttcggg
    55501 tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt tgaactgatc aaagtgccgg
    55561 ttaaatcgca taccaatcac gggcgcaatg cggagtattt tgcctgggtg caaaaacatt
    55621 tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa aatgccgggg ctggacgttt
    55681 attatgccgc tgatgtttgt tatgccgaga aagtagcgca ggaaaaaggc tttttctatc
    55741 gcctgacgtc acgttatcgc cattatgccg cctttgagcg ggcaaccttc gaacagggca
    55801 agccgacaca gctgctgatg ctgacagata agcaaatcgc cgatttccag aaacattatc
    55861 agactgaagc ggagcgtttt catattctgc caccggggat ttatcctgat cgtaaatata
    55921 gccagcagcc agccaatagc cgtgaaatct tccgtaagaa gaatggaata accgaacaac
    55981 aatatttatt gttgcaggtc ggttcagact tcacgcgtaa aggtgtcgat cgttccattg
    56041 aagcacttgc ttcgttaccg gattcgctgc gccacaacac attgctatat gttgttgggc
    56101 aggataaacc gcgaaaattt gaggcgctgg cagaaaaacg cggcgtgcgc agtaatgttc
    56161 acttcttctc ggggcgcaac gatgtctcgg aattaatggc ggcggcggat ttattactgc
    56221 atcctgccta ccaggaagcg gcgggaattg tgctgctgga agcgattact gcaggattac
    56281 cggtactaac aacagccgtt tgtggctatg cgcattatat tgtcgaggcc aattgcggcg
    56341 aggctattgt tgagccattc cgccaggaaa cattgaatga gattttacgc aaagcgttaa
    56401 cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg acattatgct gatacacaag
    56461 atttatacag tctgccagag aaagcggcgg atatcataac gggtggtctg gatggttgaa
    56521 cttaaagagc cgtttgccac gttatggcgc ggtaaagatc cttttgagga agttaaaacc
    56581 ttgcagggtg aggtatttcg tgaactggaa actcgccgta ctctgcgctt tgaaatggcg
    56641 ggcaaaagct attttctcaa atggcatcgc ggcacgaccc tgaaagagat aatcaaaaat
    56701 ttactctcat tgcggatgcc agtattaggc gcagaccgcg aatggaatgc gattcatcga
    56761 ctgcgggatg tcggcgttga tactatgtat ggggtggcat ttggcgaaaa aggcatgaat
    56821 ccgctgacca gaacttcatt tattattacc gaagatctga caccaaccat aagtctggaa
    56881 gattactgtg ctgactgggc gactaaccct tcagatgttc gcgtaaagcg tatgcttatt
    56941 aagcgtgtcg cgacgatggt gcgcaatatg catgctgtgg gcattaacca ccgtgactgt
    57001 tatatctgtc atttcctgct gcacttgcct ttttccggta aggaagagga gttaaaaatt
    57061 tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg ttccacgtcg ttggcgcgat
    57121 aaagatctta ttgggcttta tttttcatcg atgaatatcg gcctgactca gcgggatatc
    57181 tggcggttta tgaaagtgta ttttgccgcc ccgcttaaag acattctcaa gcaggaacaa
    57241 ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca gggaaagaac gattcgaaaa
    57301 tcgttgtaac ccgtaactta tttttgccaa aattttggat acagaataaa tatgtctcaa
    57361 ctcaatgata gtgacatcat cctttttgag tataattttc attatcaaaa tataagatct
    57421 aaaaatactc ttgatatagc atttggtatt gacagaaatt ttctttttgg atgtggtgta
    57481 gccatcgcat ctattctatt aaacaataga gaaatctctt gtgaattt
//
LOCUS       DAZGBC010000011        54186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGBC010000011 DAZGBC010000000
VERSION     DAZGBC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.011"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2293)
                     /gene="pbpC"
                     /locus_tag="ACHK1J_000705"
     CDS             complement(<1..2293)
                     /gene="pbpC"
                     /locus_tag="ACHK1J_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO7910029.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARQGKAGKLRLQPDDPLLERPLMSSEAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQAQQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIAS"
     gene            complement(2294..7255)
                     /gene="yfhM"
                     /locus_tag="ACHK1J_000706"
     CDS             complement(2294..7255)
                     /gene="yfhM"
                     /locus_tag="ACHK1J_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO7910030.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLEVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRCEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSTEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7462..8307
                     /gene="sseA"
                     /locus_tag="ACHK1J_000707"
     CDS             7462..8307
                     /gene="sseA"
                     /locus_tag="ACHK1J_000707"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO7910031.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8667..8792
                     /gene="timP"
                     /locus_tag="ACHK1J_000708"
     CDS             8667..8792
                     /gene="timP"
                     /locus_tag="ACHK1J_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO7910032.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNLSLSGNNLLVVNNLQNSR"
     gene            complement(8824..9600)
                     /gene="sseB"
                     /locus_tag="ACHK1J_000709"
     CDS             complement(8824..9600)
                     /gene="sseB"
                     /locus_tag="ACHK1J_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO7910033.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDVEEIIQAAGSVATDTLPADEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9743..11026)
                     /gene="pepB"
                     /locus_tag="ACHK1J_000710"
     CDS             complement(9743..11026)
                     /gene="pepB"
                     /locus_tag="ACHK1J_000710"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO7910034.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVFACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11085..11285)
                     /gene="iscX"
                     /locus_tag="ACHK1J_000711"
     CDS             complement(11085..11285)
                     /gene="iscX"
                     /locus_tag="ACHK1J_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO7910035.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11297..11632)
                     /gene="fdx"
                     /locus_tag="ACHK1J_000712"
     CDS             complement(11297..11632)
                     /gene="fdx"
                     /locus_tag="ACHK1J_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO7910036.1"
                     /translation="MPKIVILPHQELCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11634..13484)
                     /gene="hscA"
                     /locus_tag="ACHK1J_000713"
     CDS             complement(11634..13484)
                     /gene="hscA"
                     /locus_tag="ACHK1J_000713"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO7910037.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13501..14016)
                     /gene="hscB"
                     /locus_tag="ACHK1J_000714"
     CDS             complement(13501..14016)
                     /gene="hscB"
                     /locus_tag="ACHK1J_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO7910038.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14112..14435)
                     /gene="iscA"
                     /locus_tag="ACHK1J_000715"
     CDS             complement(14112..14435)
                     /gene="iscA"
                     /locus_tag="ACHK1J_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO7910039.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14452..14838)
                     /gene="iscU"
                     /locus_tag="ACHK1J_000716"
     CDS             complement(14452..14838)
                     /gene="iscU"
                     /locus_tag="ACHK1J_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO7910040.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(14866..16080)
                     /gene="iscS"
                     /locus_tag="ACHK1J_000717"
     CDS             complement(14866..16080)
                     /gene="iscS"
                     /locus_tag="ACHK1J_000717"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO7910041.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16192..16680)
                     /gene="iscR"
                     /locus_tag="ACHK1J_000718"
     CDS             complement(16192..16680)
                     /gene="iscR"
                     /locus_tag="ACHK1J_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO7910042.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(16951..17691)
                     /gene="trmJ"
                     /locus_tag="ACHK1J_000719"
     CDS             complement(16951..17691)
                     /gene="trmJ"
                     /locus_tag="ACHK1J_000719"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO7910043.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17810..18613
                     /gene="suhB"
                     /locus_tag="ACHK1J_000720"
     CDS             17810..18613
                     /gene="suhB"
                     /locus_tag="ACHK1J_000720"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO7910044.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18758..19612
                     /locus_tag="ACHK1J_000721"
     CDS             18758..19612
                     /locus_tag="ACHK1J_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7910045.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKNGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            19803..21083
                     /gene="csiE"
                     /locus_tag="ACHK1J_000722"
     CDS             19803..21083
                     /gene="csiE"
                     /locus_tag="ACHK1J_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO7910046.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVCFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21075..22214)
                     /locus_tag="ACHK1J_000723"
     CDS             complement(21075..22214)
                     /locus_tag="ACHK1J_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO7910047.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22374..23264)
                     /gene="hcaR"
                     /locus_tag="ACHK1J_000724"
     CDS             complement(22374..23264)
                     /gene="hcaR"
                     /locus_tag="ACHK1J_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO7910048.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23400..24761
                     /gene="hcaE"
                     /locus_tag="ACHK1J_000725"
     CDS             23400..24761
                     /gene="hcaE"
                     /locus_tag="ACHK1J_000725"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO7910049.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24758..25276
                     /gene="hcaF"
                     /locus_tag="ACHK1J_000726"
     CDS             24758..25276
                     /gene="hcaF"
                     /locus_tag="ACHK1J_000726"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO7910050.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25276..25596
                     /locus_tag="ACHK1J_000727"
     CDS             25276..25596
                     /locus_tag="ACHK1J_000727"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO7910051.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25593..26405
                     /gene="hcaB"
                     /locus_tag="ACHK1J_000728"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25593..26405
                     /gene="hcaB"
                     /locus_tag="ACHK1J_000728"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO7910052.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAAIGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26415..27617
                     /gene="hcaD"
                     /locus_tag="ACHK1J_000729"
     CDS             26415..27617
                     /gene="hcaD"
                     /locus_tag="ACHK1J_000729"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO7910053.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27714..28136
                     /locus_tag="ACHK1J_000730"
     CDS             27714..28136
                     /locus_tag="ACHK1J_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7910054.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28184..29056)
                     /locus_tag="ACHK1J_000731"
     CDS             complement(28184..29056)
                     /locus_tag="ACHK1J_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO7910055.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNHFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29068..30162)
                     /locus_tag="ACHK1J_000732"
     CDS             complement(29068..30162)
                     /locus_tag="ACHK1J_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7910056.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMEHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(30195..31193)
                     /locus_tag="ACHK1J_000733"
     CDS             complement(30195..31193)
                     /locus_tag="ACHK1J_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7910057.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31217..32728)
                     /locus_tag="ACHK1J_000734"
     CDS             complement(31217..32728)
                     /locus_tag="ACHK1J_000734"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO7910058.1"
                     /translation="MFTATEAVPVAKMVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSGLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEIRALRHKP
                     KLDDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITHPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASRILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32751..33734)
                     /locus_tag="ACHK1J_000735"
     CDS             complement(32751..33734)
                     /locus_tag="ACHK1J_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO7910059.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQFVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEITQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33831..37112)
                     /locus_tag="ACHK1J_000736"
     CDS             complement(33831..37112)
                     /locus_tag="ACHK1J_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO7910060.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDSLDYRCNLALAMLEYNR
                     ADFPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASKLL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKALGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37230..38423
                     /locus_tag="ACHK1J_000737"
     CDS             37230..38423
                     /locus_tag="ACHK1J_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7910061.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(38486..39739)
                     /gene="glyA"
                     /locus_tag="ACHK1J_000738"
     CDS             complement(38486..39739)
                     /gene="glyA"
                     /locus_tag="ACHK1J_000738"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO7910062.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40067..41257
                     /gene="hmpA"
                     /locus_tag="ACHK1J_000739"
     CDS             40067..41257
                     /gene="hmpA"
                     /locus_tag="ACHK1J_000739"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO7910063.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41302..41640)
                     /gene="glnB"
                     /locus_tag="ACHK1J_000740"
     CDS             complement(41302..41640)
                     /gene="glnB"
                     /locus_tag="ACHK1J_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO7910064.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41701..43035)
                     /gene="glrR"
                     /locus_tag="ACHK1J_000741"
     CDS             complement(41701..43035)
                     /gene="glrR"
                     /locus_tag="ACHK1J_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO7910065.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEMFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMVRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43025..43738)
                     /gene="qseG"
                     /locus_tag="ACHK1J_000742"
     CDS             complement(43025..43738)
                     /gene="qseG"
                     /locus_tag="ACHK1J_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO7910066.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDALRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(43903..45330)
                     /gene="qseE"
                     /locus_tag="ACHK1J_000743"
     CDS             complement(43903..45330)
                     /gene="qseE"
                     /locus_tag="ACHK1J_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO7910067.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(45906..49793)
                     /gene="purL"
                     /locus_tag="ACHK1J_000744"
                     /gene_synonym="purI"
     CDS             complement(45906..49793)
                     /gene="purL"
                     /locus_tag="ACHK1J_000744"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO7910068.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPVHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIYADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50051..51607
                     /gene="mltF"
                     /locus_tag="ACHK1J_000745"
     CDS             50051..51607
                     /gene="mltF"
                     /locus_tag="ACHK1J_000745"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO7910069.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQHMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51604..52107)
                     /gene="tadA"
                     /locus_tag="ACHK1J_000746"
     CDS             complement(51604..52107)
                     /gene="tadA"
                     /locus_tag="ACHK1J_000746"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO7910070.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52165..52800)
                     /gene="yfhb"
                     /locus_tag="ACHK1J_000747"
     CDS             complement(52165..52800)
                     /gene="yfhb"
                     /locus_tag="ACHK1J_000747"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO7910071.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53009..53857
                     /locus_tag="ACHK1J_000748"
     CDS             53009..53857
                     /locus_tag="ACHK1J_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO7910072.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
BASE COUNT        12761 a        15008 c        13702 g        12715 t
ORIGIN      
        1 ctgacgcgat ttgccctacg tcatccatca ccagtaattg ataatcacct ttatccgtca
       61 aatgcagggt cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc
      121 gttcacctgc ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac
      181 gtttaataat ctcgccatcg cgaacgcctg tcagctgcag cgggagctgg gcatcgtgac
      241 cgtatggcgg gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg
      301 gcaaccaggg ttccagcggt agcggccaga catttatcat ttcttgttgc gcctgagggc
      361 aatcagcggc aacacgtttg ccattttcat ccagccagat ggggaaacga atgccattaa
      421 tgccttcctg ctccggcagt aatagagttg gcggctgact cccgtccagc agccaggttg
      481 ccaggcggcg gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaaa
      541 taacgccacg agtgactgag ttcgggcgcg ggtcttccgg cagattagca ctgcgcgaca
      601 gtaagatatt attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa
      661 caacgggcgt gccgtccggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc
      721 caatcgccca ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga
      781 cgcgcggcaa ggcaccatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga
      841 tccacgcagc ctccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc
      901 gcaatttgcc tgccttgccc tggcgagcaa acgcggtata cgctgccgcc atatcttcca
      961 gttttgcacc agcgccgccg agaatgagcg aaagattcgg cgccgcaccg ttgggcaaat
     1021 ataacggcaa tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca
     1081 gtacctgcac agcaggtaaa ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc
     1141 catgaaaacc gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt
     1201 gcaacagcga tgccggatgg atcaagcctt catccagcgc cagaccgtaa acaaacggtt
     1261 tgagcaccga ccctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat
     1321 catcgttgag atcaaccgaa cccacccagc cgcgaacacg catatcggta tgatcaacca
     1381 cgatcatcgc cagtgagctg cgcggtggta atcgtccttt ccagttttgc gccagttctt
     1441 ccagacgtcg ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac
     1501 cgagcatcat gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga
     1561 tgggttcttc ccttgactct tttacctgct cacgggacca cacaccttgc acggccatcc
     1621 gttcgagcac tttattacgc gcgacttcgg cacgctccgg ccaacgatcc gggcgaagac
     1681 ggctgggcgc ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag
     1741 gcgactttcc gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg
     1801 gagcgcggtt aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca
     1861 acgcgcgcca gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac
     1921 gagcaacctg catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt
     1981 gccatgctgc gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat
     2041 cttcataatt gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat
     2101 aacgccagat gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga
     2161 ccactcgtgc gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg
     2221 ctgcaagaaa aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta
     2281 acaagcgagg catttacggt ctgacaatca gcaaatcttc agccgcgccg gtcgcccgcc
     2341 attggggaac atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca
     2401 ccgcccgcgc cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca
     2461 caaagcgatc gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta
     2521 agttctgcac ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt
     2581 tttccagttc cagacccgca ggcagcagat ccacgactaa cgcatccggc acgctgttac
     2641 tggcttttac ctgcaaccag accagcacca gatcgccgct acgtaacgag tccagcgatt
     2701 tgctcttacc atcagtacca agaatatgac gctcaatttg cagcacattg ttcgccggta
     2761 aaggtgcgga ttgcggataa ccgctggcat ccacacgcag ccataacggc tgctcgccac
     2821 tgttggtcac ctgcaaggtg gcaagttgat cgctattcag attgctgttt tgcgctttct
     2881 cgcctgtcag cggctcagtt gagaaagagg tttgcgcctg ccatttacct ggtaaatcct
     2941 gaatcgtacg ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac
     3001 caaacgcctg ctgcgaaagt gtgttcagca gggagtactg ctcatcgggt agcagtttgt
     3061 tctcttccag caaggagagc atcaacgcgt tgtcgcgcag tggactaccg taatcaccca
     3121 gccatatccg ctcatcacta ttacgcggcg tttttagcgc cagcgcaatc gcttcttcac
     3181 aacgcgtcgc atcacccatg gttttcagcg caacgccaag ttgcagcagc ggtaaaccag
     3241 aagcggcatc tgcacgatgc tcccagattt cacgcagcgc acccagcgga gccttttgct
     3301 gacgggccaa caccagcgca gcgtatgact gtacggcgaa tttactggct ttgagattat
     3361 ccgcgtacgg gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac
     3421 cccggttaat ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat
     3481 ccatcacgta agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc
     3541 cgccgttatc acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc
     3601 gtttctcatc actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa
     3661 gtgacgggaa caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact
     3721 ctttgatata acgtgcgatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca
     3781 gcgtaacagg tgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct
     3841 gtaacgccgt accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc
     3901 actgcttatg ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct
     3961 gaatttctcc atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca
     4021 cacctggcgc taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg
     4081 cggtcagggc aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca
     4141 gacgcgaggt atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg
     4201 cggcaactat cactttgctt tcgttgctac cgaagtcatc tgccgtccag gcttgcgcca
     4261 tgacgcgcaa ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt
     4321 cgttgagcgt taccggcagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt
     4381 taccaccacg tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct
     4441 gaccttcaat aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa
     4501 agaacgcctg ccacggatct ggcgtgacgt agtcagtaat attcagcaca ccactatcaa
     4561 cggcagacac cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa
     4621 ttttcacggt taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga
     4681 ggcgacggtt ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg
     4741 cggagcgaga tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat
     4801 tccaggtttt atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt
     4861 gccaccacag cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg
     4921 cggcaatatg caacttaatg gtatcgccag ggcgataact ggctttatct agtttcagcg
     4981 tgacacggtc gggtcgcacg gcaccgctcc cgtcgctgtt gtcctgccag ctatagccag
     5041 cccagaaacg cacactactg accgcttcat tcggcgcttt gacttccaga cgataagcgc
     5101 cccactctac cgggaaactg accttgccgg tttcgtccgc tttcagatcc agagtttgtt
     5161 cattttcaat cagatctttt tgatcaaact gtgattgcca gccttcatct tctgaccagt
     5221 tccagtagta atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt
     5281 tcacgccttg cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa
     5341 tgggctgttt caccgtgctg tcagtgcgat aatcgtaaac cgatttcgag gcgaactgcg
     5401 gacggatccc cggcaatgca tcggcaggcc agatagcctg ctcagcgcga cgcgtcactg
     5461 ggcgaccgcc cgattccagc aaactgccct ggaaaataac ctgtaatggg gaatgcgttt
     5521 ccttccactg gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa
     5581 cttcatccag cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta
     5641 aggctgacac agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat
     5701 tagcaggtgc accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg
     5761 taagcggggt tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga
     5821 cgtggaaatc ccacatccga tactggttat cgcccgtgtt agcgcgaata tgccacatac
     5881 cggttgccgc attgctatcg agtggccagg taaagtggta gaggccattt tccggctgac
     5941 taacgacgct cctgagtacc tgcccatcgg gtttaatcac ttctaacttg atgggttgat
     6001 tgggcaacgc tttaccgtct gcatcacgca gcaaaccatt gaggatgacc gtttcacccg
     6061 ggcgataaag atcgcgcggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag
     6121 caatgttaaa ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac
     6181 cgtctttacg tgccagcaat aatgcggcgt ttttatcatt ttccagctgc acatgcccct
     6241 gagcgtcact ggttgcctga gtcagagtct gccctttctc atttaataaa gagacttcaa
     6301 ttccttgctg ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat
     6361 aacggtgagc tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat
     6421 cgtaacgtcc agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga
     6481 tatcgcccag cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc
     6541 gtccggtgta gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg
     6601 aattgcggta ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga
     6661 agttaacatc gacattatta acgttgagcg ccattaccgg cagcccttca acgactttac
     6721 caggcagcag cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta
     6781 tagttttttc gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa
     6841 tagtaacgat caaatcacgt ttcggttcga ggtggcgtaa acgcagctct ttaagattat
     6901 ctgacagctc ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc
     6961 gtgagaaatc ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac
     7021 catcaagttg gacttcagag agatccagca atgtcagcgc cttaccggca ctctgttgtg
     7081 ccagtttttg tcgctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg
     7141 ctttagtaac ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt
     7201 cgcaccctgc cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc
     7261 atccctgcca caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt
     7321 tatgtaattc agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca
     7381 gccaaatcag cctcttttgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc
     7441 ccggcaaaaa tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc
     7501 gaacatattg atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag
     7561 gaagatcgta acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt
     7621 gatatcgaag cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa
     7681 acgttcgccg tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat
     7741 gacgaaggta atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta
     7801 gagaaagtgt cgattctggg gggtggactt gcaggctggc agcgcgatga tctgttgtta
     7861 gaagaaggtg cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagca
     7921 gtggtgaaag taaccgatgt gttattggca agccatgaaa atacggcgca aattattgat
     7981 gcccgcccgg ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc
     8041 ggacatattc ccggtgcatt gaacgttccg tggacggaac tggtgcgcga aggcgaacta
     8101 aaaacgaccg atgaactgga tgcgatattt tttggtcgcg gcgtcagcta tgacaaaccg
     8161 attatcgtca gctgcggctc tggtgtaaca gcagccgtgg ttttgttggc actcgcgacg
     8221 ctggatgtgc caaacgtaaa attatacgac ggtgcgtgga gtgaatgggg cgcacgggca
     8281 gatttaccgg ttgagccatt gaaataagct ttatacaggc aataaaaaac cgccgaaaat
     8341 aaaaaaccgc cgaatttggc ggttttttat tgctggtctg gttcgcggcc tttccagcag
     8401 gttgacttgt gttgccggag caacgcaggc accccacagt gttgaaatac tctcttttat
     8461 gcctggctgt gtcaagataa taaacagagt aattattcaa acataagagt attattctta
     8521 atggcccttt tcgccgtctc gcaaacgggc gctggcttta ggaaaggatg ttccatgacc
     8581 gtaaatacag gcaccccaca gtgcttgcta tcgcggcaat atcgccagtg gtgctgtcgt
     8641 gatgcggtct tcgcatggac cgcacaatga agatacggtg cttctgtatc gtacttattg
     8701 tttctggtgc gctgttaacc gaggtaaata ataacctgag tctctccggc aacaatttac
     8761 tggtggttaa caaccttcag aacagcaggt aagcccgaat gccgcccttt ggggcggcat
     8821 attttagatt atccggttct gtttaaagtc acgcaaaaaa ccaccccaac gacgttcata
     8881 gaatggcgca atatgttcgg taataaagtg gctaattcct ttttcccctt ttttcacctg
     8941 acaaatatcg attggttcat cggcaggtaa tgtatcggtc gctacacttc ccgccgcctg
     9001 aataatttct tcgacatcac catcggcttc aatgccaata agtaaattag gctgtgcctc
     9061 ttcgttctct ttaattgaac atataaaagc acgcttcacc ggcttaatag ttttaaataa
     9121 ggtggtgagt gaatcaatca tttgtgctgg cggctctgcg acttccgata atatcagcga
     9181 ttcaccgcct tccaggattt cctggctgct cagcggattt ccctcttcac caatcaacaa
     9241 actgatttca cgcggcataa attctttacc ggtcggcagt ttggcattaa ggaagagcgt
     9301 ttcgccaagg gtcatctcaa acaatgtgcg aacgggcatt acgacaaatg cctgttcgtc
     9361 ttcaaccgcc tgttgaagtg cttctaacga ggtgaaaaaa ggaataacgc tggtgccgtc
     9421 ttctttttcc cagtgctgta aatcaagcgc gctatcttca accacagcct cgccctgcgc
     9481 cgccgtacca ggcacccaga cggtggattc cagtagagta cggaaaaagg ccgggcggtg
     9541 cgccggttcg gttgctgctt tttccagcag gtcttcaagt tcattttttg tttcggacat
     9601 aagaaccaca attcattcaa cgttcggggc acaagtgcct gatgcgccat acttatcagg
     9661 cctacaaggg agtcgcaatg tattgaattt gcaatacttt gtaggccgga taaggcgttt
     9721 acgccgcatc cggcatatta gtttacgccg ttaacagatt agcaatcgtg cgcacaccaa
     9781 gtcccgtagc acccgcagac cactgttcaa ccggcgcttt acggtaagtc gccgagcagt
     9841 cgatatgcag ccagccttgc tgatagttct caacaaagtg cgacaggaag cccgccgccg
     9901 tgctcgcgcc tgccggatac gccgcgcttc cggtattgtt cagttcggca aagttagacg
     9961 gcagctggct gcggtggaac tccgccagcg gcagacgcca gaatggttcg ttctcttgtg
    10021 aggcactcgc cagcaaacga ccggccagcg catcgtcaaa actgaacagc gcatgataat
    10081 cattacccag cgcggttttc gccgccccgg tgagggtcgc cgcatcaatg atcagttccg
    10141 gtttctgcgc actggcatca atcaggccat cggccagcac cagacgccct tcggcatcag
    10201 tgttcatcac ttcaactttt ttaccgttgc gataggtgat gatatcgccc agcttgaacg
    10261 cattgccgct aatcaggtta tccgcacagc agaggaacag cttcacgcgc ttgttcagtc
    10321 cgcgcgtaat ggcaaatgcc agcgccccgg taaccgttgc cgcgccgccc atgtccgact
    10381 tcatcgagtc cataaacgca gtctgtttga tgctgtagcc gccggagtca aaagtgatac
    10441 ctttacctac caggcacgcg aacactggcg cttctttatc gccagttggg ttgtaatcca
    10501 gcgccagcaa taccggagaa cgttctgaac cgcgtccgac ggtgtgcagc cccatataac
    10561 cttgctcacg cagatcttcg cctttggtga tccgataagt cacacgatcg cccgcgacgt
    10621 tgctgatcag atcaacagca cgctgtgcca gttgcgatgg tcccaattct tctgccggtg
    10681 cgttgatggt gtcacgcacc cagtcgatga tcatcaggcg gttatccagt tcctggcgct
    10741 gggcatcgtc cagatccggc cactccactt tacgcgtgcc tttcggggct ttgtaacctt
    10801 gccagaatgc ccagcagcga tccgcatccc agccttcacc gcttagctga acatgcttga
    10861 tgcccagacc gtcaatcttg cgcgccgcac gctggatcag ccccagatcg tctgccccgt
    10921 tcaaatgcag agtaatgcca tcattattaa tgctgtaagt tgctttttct ccccagcgcg
    10981 cgtcggcagg ttgggtagag agggtaattt tcatcgcttc tgtcatttta gttatcctta
    11041 ttcgtttaac agcaaaacgg gctaccccca ggcagcccgt tcattcactc ggcttcgtcc
    11101 agccagacta acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg
    11161 aaatcttcca gatcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca
    11221 agatcgggat acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccacttaagt
    11281 cccatactaa cctctgttaa tgctcacgcg catggttgat agtgtaacgc gggatttcga
    11341 ccactaaatc ttcgtcggta acgcgcgcct ggcagcttaa acggctttcc ggctccagtc
    11401 cccaggcttt gtccagcatg tcgtcttcct gctctgagct ttccggcagc gagtcaaaac
    11461 cttcgcgaac gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa
    11521 tctcgatacc gttacgcaga gctgcgtcga gaatggtttc accgctatta gcttccagaa
    11581 cagcgccatc agggcagagc tcctgatgag gcaaaataac aatctttggc atattaaacc
    11641 tcgtccacgg aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg
    11701 aaatcctggg tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacatcatcg
    11761 ccctgcgcca cttcactcag gtgagcggca gcatcgtcaa tgacctgacg ttctgcggcg
    11821 cttaacagcg cggcatcagc agccagcgcg ccgtgcagac tttccagcac acgcgccgct
    11881 tctacttttt gttctgccag cattcgggct tttacgtcct gctcggcata gctcattgag
    11941 tctttgatca tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt gacctgaata
    12001 gacgcctcaa cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg
    12061 acctggaacg tcacgcgaat atgcgcaccg ccagccggta gcgccggaat accacgcagc
    12121 gcaaaacgcg ccagtgagcg gcagtcctgc accagttcgc gctcaccctg cattacatgg
    12181 atagacatcg ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg ggccaccgga
    12241 atagtggtat tacgcggaat cactttctcc accaggccgc ccatcgtttc gaggcccagc
    12301 gacagtggga tcacatcaag caacagcatt tcgctgtctg gcttgttacc caccagaata
    12361 tccgcctgaa tcgccgcgcc aatagcgacg actttatccg ggtcgatgga agtcagcggt
    12421 ggacgaccga aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt agaaccgccc
    12481 accatcacca cttccagcac ttcatcagct tctacacccg cgtctttcag cgcgcgacga
    12541 caagccagta aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg
    12601 atttcgccct gccagcccgc aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt
    12661 ttggctgcaa tggcggcatc cagcagttca cgctgaacgc ggttatcgct acgatcagga
    12721 atgcccgcct gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc gccgccgagc
    12781 gcggaatcac cgccggttgc cagcacttca aacacgccgc gacttaagcg cagaatggaa
    12841 atatcaaacg tcccgccacc gaggtcataa acggcgatca cgccttcctg accggaatcc
    12901 agcccgtagg cgatagccgc agcggtcggt tcgttaagta agcgcaggac gtgaaggccc
    12961 gccagacgcg ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta cgccggaacg
    13021 gtgataacga caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct
    13081 ttgaggatgt ccgcagaaac gcgcaccggg ttcagcagcc ccgccgccgt ttcaatcatc
    13141 ggcaggccgt tttcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata
    13201 tcagccagcg agcgtcccat caggcgttta acagaactaa ttgtgttggc ggtatcgagc
    13261 gctgcattag tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca
    13321 gatggcagca ggtgacggcc ttcatggtcg gctaacgttt cggcctgacc gctgcgcact
    13381 gtcgccacca gcgagttggt tgtgcccagg tcaataccgg ccgccagacg acgctgatgc
    13441 ggcgcagcac tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa
    13501 ttaaaaatcg agcagttttt cttcgagttg ttcggcactg cttcgcagtt tatcgagaaa
    13561 acgcagctta cgcacggtat ccgccgccgc gtcccaggtc tcgttgtcta actgttcaac
    13621 catcaactga tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg
    13681 cgcttcatct ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca actgttccat
    13741 caggaacgcg gtgtcgcgca cagtatgctg ctcgctggcg agatcaaagc cgtgcaaaga
    13801 aagcaaatat tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct ggttaatggt
    13861 tgcagattgc tgtacggcgg cgagttgttc cgcctggctt ccgctggcga atttatcagg
    13921 atgatactga cgttgtagat cctgaaaacg caggctcagc gcctgggtat cgagttgata
    13981 acgggcaggc aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc
    14041 cgcgataacg gcgaatcagg taaaacaaac cccacgcgca ggcgaccacg gtggggttat
    14101 cggtatgcgc atcaaacgtg gaagctttcg ccgcaaccac actcatcttt aacgttcggg
    14161 ttggtgaatt taaacccttc gttcaggcct tcttttacga agtccagctg cgtaccgtcc
    14221 agaaattgca ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg
    14281 tcttccggcg tcggttcgtc aacaaattcc agtacataag ccatacctga acatccggag
    14341 gttctcacgc ccagacgcag gccaaaccct ttgccgcggt tagccagaaa ggtatttact
    14401 cgcgctgctg cactgtcgct cagtgtaatc gacataacca aacctcaact cttattttgc
    14461 ttcacgtttg cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga
    14521 acagtgaatt ttcaccggcg gcagttcaag ttcttcagca atatcggtgt ttttgatcgc
    14581 ctgcgcttcg tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat
    14641 agcggaaccg cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac
    14701 tttaatctgc aacttcatca cgtcgccaca ggccggtgca cccaccatgc cgctgccgac
    14761 gttctcgtcg ttgttgtcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac
    14821 tttttcgctg taagccatta taaattctcc tgattccgat accgattaat gatgagccca
    14881 ttcgatgctg ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg
    14941 cagacgaccg atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt
    15001 aaaacgacct aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc
    15061 gcgcagcacg taggacggtt cgaggcttgc tgacgtacag gcggagccgg aagaaaccgc
    15121 gaggtctttc agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag
    15181 aatgttcggc gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat
    15241 gccgttccac agacggttac gcagtccgcg cagacgttcc atctcggtcg ccatctcttc
    15301 ttttgcgatg cgataggctt cgcccattcc gacgatctgg tgaacaggca gagtgccgga
    15361 acgcataccg cgttcgtgac cgccgccgtg catttgcgct tcgatgcgta cgcgcggttt
    15421 acgacgcaca tacagcgcac caataccttt cgggccatag attttgtgac cggagaaaga
    15481 catcaggtca actttcaact ggctcagatc gataggcagt ttgcccacgc tctgggttgc
    15541 atcaacgtga tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg
    15601 caccacgccg atttcgttat tcacgtgcat gatagaaacg aggatggtgt cgtcacgcat
    15661 cgccgcttcg agttctttca ggtcgataat accgttacgc tgcggtgcca ggtaggtgac
    15721 ttcgaaacct tcgcgctcca gctgacggca ggtatccagt accgctttgt gttcggtttt
    15781 gctggtgatg atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag
    15841 gttgtcagat tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag
    15901 atcggcaatc tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg
    15961 agaacgggag gccgggttac caaaggttcc gtccatcgtc ataaactgca tcattttctc
    16021 ggcaacacgc gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat
    16081 tgctctataa actccgtaca tcactcaatg caaggaatca ggctaccggc tggatgtacg
    16141 accgtgttta cggagtattt agcactccgg cctgattctg aattcttttt attaagcgcg
    16201 taacttaacg tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg
    16261 accagacaca tccagcactt cctggttatt aaccagttcg cctaaagtaa tgttgttgag
    16321 aaaaccggtg agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc atttatcccc
    16381 gccctggcag ccgcctttac cctgacaacg ggtagcatct acagattcgt caacggcgct
    16441 aatcacttcg ccaacggcga tgctgctggc atctttgcct aacagataac caccgcctgg
    16501 tccacgtacg ctggaaacca gaccattttt acgcagacgg gaaaacagtt gttccagata
    16561 agaaagggaa attccctgac gttcggaaat atcagccaac ggtaccgggc ccgcttcaga
    16621 gttgagcgca acgtcaagca ttgcggtcac ggcatagcgc cctttagatg tcagtctcat
    16681 gtcttacttc acctcaaact cgcccctgcc cggggttttt tattgtaaaa gtgggggtat
    16741 tgcatagcag ggtcaagtct gacattcccg agtaaattgg tcaactattt acttgactga
    16801 tttagtcggg tatttaacct tcagtgccat ttttttatcg tggcgtttgt acctgttgtc
    16861 ggatgcggtg tgaacacctt gtccgacctg ggattccgtg cacttacagg cctgataaga
    16921 cgcgttaagc atcgcatcag gcaaccactg ttattcggcc ttattacctt tattctgctg
    16981 ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    17041 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    17101 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    17161 atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat
    17221 acgcacttca taggcaataa cctgaaccgc cattgccagg ttcagcgaac tgtattccgg
    17281 gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    17341 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cacttttcag
    17401 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    17461 taccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    17521 aatcacatcg ctggcccccg ccgccagggc aatagcctgg gagtcaggtt tcaccagtgg
    17581 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    17641 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    17701 tcgtctaaag attattcgcg catcttatca taaaacgaag acagatgccg atctcgctgc
    17761 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    17821 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    17881 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    17941 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttacccac agcacaccat
    18001 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    18061 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    18121 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    18181 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggcagcac
    18241 cgctcgcgat ctcgacggta ctattctggc gaccggcttc ccgttcaaag caaaacagta
    18301 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    18361 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    18421 tgaaatcggt ctgcgcccgt gggacttcgc tgcaggcgag ctgctggttc gtgaagcggg
    18481 cggcatcgtc agcgacttca ccggtggtca taactatatg ctgaccggta acatcgttgc
    18541 tggtaacccg cgtgttgtta aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    18601 tctgaagcgt taatgactca ggcgggtgat atcactcacc cgccctcgcc tttcaggcgc
    18661 tattccgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    18721 cgattaatgg atgataaaga agtcgcaagg aaaggttatg gcactgccag tgaacaaacg
    18781 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    18841 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    18901 ggcggaatcc gtcgaattta cggctaagaa cggtactcgt ctgcaaggct ggtttatccc
    18961 ttcttcgacg ggccctgctg acaacgccat cgcaaccatc attcatgctc acggcaatgc
    19021 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    19081 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    19141 gctggacgat acgcaaagcg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    19201 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    19261 gggtgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    19321 catcgccaac cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    19381 aaattacatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    19441 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagagc caaaacggtt
    19501 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    19561 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    19621 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    19681 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    19741 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    19801 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    19861 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    19921 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    19981 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    20041 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    20101 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    20161 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    20221 tgcaaaaacg ctttgaatcg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    20281 tgctgcagca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    20341 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    20401 tccagcctgt accacctgat gagccactgt ttatggcact acttttttcc atgttgcggg
    20461 ttcccgatcc attgcgggat gcgcatcagc gggacagaca attgcgtcag tctatcaaac
    20521 gtctggtaaa ccattttcgt gagctgggaa atgtttgttt ttatgatgaa caggggttat
    20581 gtgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    20641 ttgataatac cctgccggaa gagttcgcca gactgtatcc acgcctggta cgcaccaccc
    20701 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagtg
    20761 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    20821 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    20881 gcgaactaac gttactgccg ctcaacatta aacatatgtc ggtaaaggca tttttgcaga
    20941 caggcgctcc gcgtggcgcg gcactgatta ttgctcctta taccatgccg ttaccgctct
    21001 tttcaccacc gctgatctat acggacctga cactgacaac acatcaacag gagcagatcc
    21061 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    21121 gccaccagcg ccatcaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    21181 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    21241 cggatgactt cgctaccctg gcgagcggca atatagcgca tggcggccag gtggcagacc
    21301 gtgaaggtgc cgcaatgcag aatttgcacc actatcaacc acggcaacgc cgtagttgct
    21361 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgtgca
    21421 ctacaacggc ggaaaagttt attactcagt gcaaagataa tgacttccgc caccacgccc
    21481 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    21541 ctaaaaccgt aataggccgc atgcgccccc tgcaataaac aaacgcaggc cagaaagcgc
    21601 cagttctggc gaaccagcgc caaccacgct gaccaaccgg tgctctcctg ttggcggctt
    21661 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    21721 aacgtcaaca gcacgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    21781 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    21841 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    21901 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    21961 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgagaagga
    22021 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    22081 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    22141 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    22201 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggagtta atcgaatgtt
    22261 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    22321 acaagcactc aaaaactgcg atccgtgacg catgttgcaa agataatgtg taattatgcc
    22381 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    22441 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    22501 aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    22561 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    22621 tggctgtttt cttgtgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    22681 ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc tttttcatgt
    22741 gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa
    22801 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    22861 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    22921 ctgagacgaa acatcggtaa tacttttggc agtaaattca cttccgccga tggcacaaag
    22981 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    23041 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    23101 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    23161 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    23221 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    23281 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccagca
    23341 acatccttat ggcacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    23401 tgaccacacc ctcagatttg aacatttacc aactgattga cacccaaaac ggtcgggtca
    23461 ctccgcgtat ttataccgac ccggacattt accaactgga gcttgagcgt attttcggtc
    23521 gttgctggct atttctcgcc cacgaaagcc agatcccaaa acccggtgat ttctttaaca
    23581 cctacatggg agaagatgca gttgtcgtgg tgcgccagaa agacggcagc atcaaggcgt
    23641 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    23701 gcgcctttac ctgcccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    23761 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg gggctaaacg
    23821 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    23881 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    23941 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgcaactgga
    24001 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    24061 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    24121 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    24181 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    24241 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    24301 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca
    24361 cgctccgcgt ctggcatccg cgcggccctg atcaagtcga agtgtgggcg ttctgtatta
    24421 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    24481 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    24541 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    24601 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    24661 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    24721 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    24781 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    24841 atttcgtgac tggctggcgc agctcgacga agagattcgt tacaccatgc gcaccacggt
    24901 taacgcgcaa acgcgcgacc gccgcaaagg cgtccagccg ccgacgacct ggatttttaa
    24961 tgacactaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    25021 agaagagcca ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    25081 catcccaaac gtatttgctg ttcgggtgaa ttatctgctt tatcgggcac aaaaagagcg
    25141 cgatgaaacg ttctatgtcg ggactcgttt tgacaaagtt cgccgtctgg aagatgacaa
    25201 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    25261 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    25321 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    25381 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    25441 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    25501 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    25561 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    25621 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    25681 gcgcgcaggt tgccacgctg gaactgtcgg cggcgaaagt cgccagtctg cgtcagcgat
    25741 ttggtgaaca tattctggcg gtggaaggca acgtgacctg ttatgccgat tatcaacgcg
    25801 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcgggca
    25861 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    25921 acgagctgtt taacgtcaac gtactcggtt acctgctggg tgcaaaagcc tgcgctccgg
    25981 cgttaatcgc cggtgaaggc agcatgattt tcacactatc aaatgccgcc tggtatcccg
    26041 gcggcggtgg cccgctgtac accgccagta aacatgccgc aatcgggctt attcgccaac
    26101 tggcttatga actggctccg aaagtacggg tgaatggcgt cggcccgtgt ggtatggcca
    26161 gcgacctgcg cggcccacag gcgctcgggc aaagcgaaac ctcgataatg cagtctctga
    26221 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    26281 cgggtccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    26341 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagctgct gggctggatc
    26401 tttaaggaag tacgatgaaa gaaaaaacga tcattattgt cggtggtggg caagcggcgg
    26461 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    26521 atgagcaaca tcttccttat gaacgccctc cgctctcgaa atccatgttg ctggaagatt
    26581 ccccacagtt gcagtctgtg ttacccgctc actggtggca ggaaaacaat gttcatctgc
    26641 attccggtgt aaccatcaaa acattgggcc gcgacacacg agagttagtg ttagccaacg
    26701 gcgaaagctg gcactgggat cagcttttta tagcaaccgg cgcggcagct cgaccgctgc
    26761 cgttgcttga tgcactggga gaacgctgct ttaccttgcg ccatgccggt gatgccgcca
    26821 gactgcgaga agttctgcag ccagaacggt cagtcgtaat tgtcggtgcc ggaactattg
    26881 gtctggaact ggctgccagc gccacgcagc gcggatgtaa ggtgacagtg attgaactgg
    26941 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    27001 atcaacaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    27061 aaaaagtaga actgacgctg caaagtggcg agacgcttca ggccgatgtg gtgatttacg
    27121 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actaccaatg
    27181 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    27241 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    27301 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgcttccg ctactgccgc
    27361 cgccgtggtt ctggagtgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    27421 gcgatgactg gctttgtcgt ggcaacccgg aaacccagaa agcgatttgg tttaatctgc
    27481 aaaacggcgt gcttatcggt gcggtaacgc tgaatcaggg acgtgagatt cgctcaatcc
    27541 gcaaatggat ccagagcggc aaaacgtttg atgcgaaaca gctgacagat gagaacatcg
    27601 cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg gcgtggcgag
    27661 gtaccagagt gtgtattacg tttaaatcac attatcttgc aaagggattg gttatgaact
    27721 cactacgtta ttttgatttt ggtgctgccc gccccgtttt gttattaatt gcccgtatcg
    27781 ccgtggtcct aattttcatt atttttggtt ttcccaaaat gatgggcttt gacggtacgg
    27841 tccaatatat ggcctcgctg ggcgcaccga tgccgatgct ggcagcgatt attgcggtag
    27901 ttatggaagt gcccgccgcg atattaatcg tgcttggctt tttcacccgt ccgctggcgg
    27961 tactgtttat tttctacacg ctgggtacgg cggtgattgg tcaccattac tgggatatga
    28021 ccggcgatgc cgtcgggcca aatatgatta atttctggaa gaatgtcagt atcgctggcg
    28081 cgttcttgct attggcaatt accgggccgg gggcaatttc tctcgatcgc cgttaggaaa
    28141 gatgccggat gcggcgtgaa cgccttatcc ggcattttat aaattacagc cactctaccc
    28201 gcaacgacat ctctgaggtt gttgattccc ccggcgcaag cgcaatgagg tcaccccctt
    28261 ccgggcgatg atggtcatcc ggcgcatggc tcatcggctc cagacagaag aaatcaaacg
    28321 catatccttt atcaaacgcc gggtcggaaa caaagatgaa ataacacggt gcaggtggcg
    28381 tcgtttctat gatgatggca taaccctcct gcggctgctc gatgcgggcc tgaccattcc
    28441 atccggcaaa accattgttc acccactggc gcggcaacgg cgctagctgg ttaaaatcca
    28501 gttcttgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgc tccagccagt
    28561 aaccgctcgc ctgtgcctga atccgcgttt gcggcgacaa cggaaaataa ggatgccagc
    28621 cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agagagcgtc accgtcagcg
    28681 tatccgccgt taaatgaaac gcctgactta cccgatagtg atagacaccg ctgcgatgtt
    28741 catacaccag acacaaacta tcatcgctgt gcgagacaca ttgccactcg cccagccagc
    28801 catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga tactcacgcc
    28861 cctgccagac aaaatggttg ccgctcaccc ggtttgcaaa tggcactagc ggaaaacaag
    28921 aggcatcggt tgccacaccg ctttttttac caggacgtaa gagcggcgtc gtatcacgcc
    28981 agaaaccttc gatgacaccg ccctggtcag agacatccag ctttagcggt ccgtgagata
    29041 aggtatagat ggtcatccgc taactcctta atccgggaag ttaatcacaa ctttcccgca
    29101 tttgccgctc gccatcagcg cataggcatc acctgcctgt tccagcgaga agcgatgggt
    29161 aatggcgtta cgcggccaca gcttccagtc agtcaaatcg tgggcgcatt tttccatatg
    29221 gaacagactg gtcacccagg agccaataat ccgccgttga tggtgcatca gatcggcgct
    29281 gacctcgaat tccacttttc cggtttcacc aatgtaaacc acccgccccc agtcagcagt
    29341 ggattgcagt gccagcaagc gacctgcggc attaccggaa caatcgagcg caacatccgc
    29401 gccaccgtgg gtaagttcag caattatctg cggcagacct tcggtggtcg ctaaataacc
    29461 gtgctccatc acccctaact gttttgccat cgccagacgt tccggcagca tatcaacgcc
    29521 gatgatccgt tttgcaccgc gacctttcgc cagcatcatc gccatcatgc cgaccggccc
    29581 cagaccgacc accagcacgt tatcgctgcc ggaaacttcg ccgcgcaaaa tcccttcata
    29641 ggccgtgcca acgccgcaac tgataaacgc accatcttcg tagctcagcg catccggcag
    29701 gaggatcaga tctttttctt ccgccagcag atattcggca tgaccgccgt cacgctgcca
    29761 gccgtaagcc gcttttcctt tgccagtaca agagatagga aagccgcggc ggcagttcgg
    29821 gcaaaaacca cagccagaaa tgtgatacac cagcacgcga tcaccctctt taaaatggcg
    29881 acagccttgc cccatcgcca caatttgccc gcacggctca tgaccgttga taaagccctg
    29941 gtataacggt ttatcgggcg ccgccgccgt ggcgcggtgt tgatggtaga tatagtggac
    30001 atcgcttccg caaatcccgg aggatttcat tttgatcagt acctggttaa tccccggcgt
    30061 cggcaccgca acttcccgca gatcgacggt cgaatttcct ggtaaataag ctgccagcat
    30121 cgttttcata aatccctcat taacaatacg attaattttc atccctgccg cacccgcgcc
    30181 aggggcgtta aggtttagcg tttcgcttta ctgctacgct gggtcagcaa gatattcgcc
    30241 agcaccgcca ccacgatgat gacgccgcgc accacctgct ggaaaaagga gttaatcccg
    30301 agcagcacca gaccgttacc gattagcgta atcaccagta cgccaagcag cgtaccgaac
    30361 aacgagccgc gaccaccgga aagcgccgta ccgccgacca cgaccgcggc gatgacgtca
    30421 aactccagac cgtttgcggc acctgcgtta ccggaaccga ggcgcgccgc caacaaaatg
    30481 ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa gaatgcgtac ccgacgaacg
    30541 ttaataccgc acaactgcgc cgccgtggca ttaccgccaa cggcaaaaac cgagcgcccg
    30601 aaggcggttt ttcggctaat gaacacaaac agcgcaaaca gcaccatcat gatcagcgcg
    30661 gataccggca caccgagaaa ttgtccgccc agccagtcca gcacctcgtt ttcgtcaatc
    30721 ggcactggca gggcgttggt cataaacaag cccattccgc gcagggcgct ccacagcccc
    30781 agcgtggcca cgaaacttgg cacgttaaac acgccacgca gcaccccggc gagcgttccc
    30841 atcagcgcgc ctaacagcaa caccagcaga caagcaatcg ccaacggaac ttcaaattgc
    30901 agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg gcccaacgct gacatcaatt
    30961 tcaccggaga taataatcag cgtcatcgcc caggcggcaa tcccaatggt ggcggcatcg
    31021 cgcagcacgt tcatctggtt attcaatgag ataaagccag gcgcgttcag ggagaagacc
    31081 agataaagaa tggcaatcac caccagcaaa ccgatctcat taatatggcg actgacaaat
    31141 tgtttgagcg agacgctctt gccctgcggc aatggtaatg acgaagcaga catcttaggt
    31201 tcctcggtaa aattcatcag tgcacagaca gaatggcgga catcagctca tccacattga
    31261 ccggagcgtg aaactcctgc gagaacgtac cgtgctgtaa caacagaatg cggtcgcaca
    31321 ccagcggtaa ctcctccact tcactggaga taaacaccac gctttttcct tcggcagcca
    31381 gctcacggac aatacggtaa atctgctgtt tggcttcgat atcgacgccg cgcgtcggct
    31441 cgtcgagcaa caaaatccgg ctggcggcat agacccaacg accgatcacc actttttgct
    31501 gattgccgcc agaaagcgta ccgatgggcg tttcactact ggcggccttg accgtcatcc
    31561 gctgcatcac ctcttcggtt aggcggcgga tggtggacca ttgcagcaca ccgttggcgc
    31621 tgattttttg ccgattggtc agcactgtat tttcgtcaac gcccaaccag ggaatgatcc
    31681 ccgcttcttt gcggttttct ggcgtatagc caatgccgcg tttcagcatg tcgccgtaat
    31741 cggggtgcgt gattttctcg ccgttgataa caatttcgcc ctgttcatac tcctccagcc
    31801 caacaatcgc tttcagcaat tcactgcgtc ctgcccccag caggccagca atgccgagca
    31861 cttcgccacg acgtagcgta aagctgatgt cgtccagctt gggcttatgg cgtaacgcac
    31921 ggatttccag cacggcctga tccacaattt cctgaggggc aaccggcgca atatcaacgt
    31981 gatcgcgccc gagcatcagc gacacaatat gatgcgtgga ggtgttttcg agcatcacat
    32041 cgcccgccac ctgaccatcg cgcatgacgg tggcacagga ggcaatgcgg cgaatttctt
    32101 ccatccggtg gctgacataa atcaccgcca cgcccagtgc cgacatcttt ttcacggcgc
    32161 ttatcaccag ttcaacttcc gcactggcaa gcgaactggt aggttcatca agaatgacca
    32221 cgcgcggttc gcctttcatc acccgcgcaa tttccaccag ctgcttttgc gccgggctta
    32281 gccctgaaac aagttgttca gggctaacgt caacgcccag cgcctgtaag caacgttggg
    32341 catcctgcgc catttgcagg taatcaatca tgccgttgcg gcggggccac tgaccgaggc
    32401 agaggttttc cgccactgtc agcccttcca ccaaacttaa ctcctgataa accgcgcgta
    32461 cccccagttc agcggcacgg cgagtcaacg tagcttcgtc gccttccagt cgcgtctcac
    32521 caatccagat atcaccgcta tccggacgtt cgctaccggt aagcattcga atgagggtcg
    32581 atttgcccgc gccgtttttg cctaacagcg cacgaacctc gcctttattg agcgtgaagt
    32641 taacgttatc caacgcaacg acgccgggat aacgcttatt tcctgccacc atttttgcta
    32701 ccgggactgc ctctgttgcc gtgaacatag ggacctctgc gaatcagcga ttagggcaga
    32761 ccatcaacgt gcgttgccag ccactgttta ccgtcttccg ttttggtata gagatcgatt
    32821 ggcacctgaa tcactttttc accgtcggct tgtttattga taaccttcaa tgtttgcgcg
    32881 aaaacagcat tgcccatttt cttaccggaa atatccacta ccgctttcag cacctgattg
    32941 ttttccagct cctgagtaat ttcggttgtc atatccgaac cgaaaacagc aatttttccg
    33001 gcctgatttt gattacgtac cgcttttacc gcgccgagtg tcgcaccgcc cgattccccc
    33061 ataatggcgt tgagatccgg cgtggagata atcagttttt caccaacgga aatcgcttta
    33121 tctaaaacag tcccttcctg attagcgaca aattgcgcgc cgggaacgcg ggcttttaat
    33181 acttcttcaa accctttacg tcgttgcaca caaacttcaa aggcttcgca attgatgacg
    33241 gcaattttcg gctggtcaat tttgttggca ataaaataat cggcggcagc gttacccagt
    33301 tttttaccaa attccagcgg gtcacccacc agatatgccg agacatattt atcgaccccc
    33361 ttttgattaa tacaggtgtt gtagcaaatc accggaatac ccgcttcact ggcgcgacga
    33421 acggtacggc tactgccatt ttcagacact gccgataaaa taatggcatc gacattacgc
    33481 gccacgaggg tatcaacaaa ggtactttct ttcgaaatat caccctgggc gttagtttca
    33541 attaactgta cctgtactga agaatctttt gccgcatcct gaacgccctg acgcactcca
    33601 gcgtaatatc cctgagtatc aaggtatatt gcgccaatgg tcatttcttt ttccgcagcc
    33661 ctggcaaata aagcgctacc taatagcgta gccattaata atagattacg ggttgttctc
    33721 atttttttag gcatagagcc tcctgtaggg tttttattaa caacggctta ttctaattat
    33781 tttgtgatga gcggcagcgc gtgccgctca aatattacag ttaatagggt ttagtgaacg
    33841 tagttaaaaa taaaaggcat cacggtggtg aataatgccg ctttcggcca ggccggatta
    33901 atcgccgtca attcagcgcg agcagactca tattgcgcaa cctccccgag tcccgcggac
    33961 gccagcatcg ccaccatcag gcatttttct ttatgctgct gttgtaaatc gccatacagc
    34021 gacaacagat caggttgtga aacagcaaag aaatccgcct caatactggt tttcgccatc
    34081 tcttgcgccc actgtttcat ttcactaaac agttgctgtg cggtgtgttg ttcgcccagt
    34141 aatcgcagcg ccattccttg ccagaagagg taatcaaccg gctgatcgtt gtaatagctg
    34201 tggatgttaa tggtgcgatc gccggtcgcc gccagacgta aacagcgcgt cgcttcagtt
    34261 tcatcaccca gggctttggc acatatcgcc tgccagaacc agatgtcgtt gtcagtttgc
    34321 cccggtaatc ggccttcgct taaattctcc ggatagtgca gcgcggcatg aagcagtttg
    34381 ctggcctgct gcggttgtct ggcatcaagg tgttgccagg cgcgtaacaa ctggttgagg
    34441 ataaactgac tggtgacttt cccttctccg ccttcccacg ggtggaattt gcgcgtggcg
    34501 agaatgtccg ccgctttgtc tgcctgcccc gtgagatgcc acaaattgag cagttctgcg
    34561 gtcatgtcgt cgcgtttcag cgcaatttcc agattatttt ccagacgcgc cagtcgtttc
    34621 tccggtgtgg ctccacttaa cttatcaagc aaatcccgtt cgaaaagcag acgtgcatcc
    34681 tgcggcgcaa gctgataagc attatcaaga taacgcgcgg ccagctcata atcgtgttgc
    34741 ttattccacg catggatcgc taacccgcgc cagccgtcgg caaactccgg cgacatctct
    34801 acgcaacgtt gccataaggc aatggctttg ttgtagctac gtttgttgta gtagaagcag
    34861 gccagtaaat ggcgagcaaa ccagcactct tcaatactct ccagcgccgc cacttcttcc
    34921 agcgtattcg ggaaacggac aaactgcggg aagacatcaa tggcttttgc gaccagttcg
    34981 ccacgttcgg ctttcggcag caagctggct tgcaggtaaa gcggcagcgt gcgctggcag
    35041 tccagtgcgt tcagcatctc tgccgccagg gtgggcattc cccagttaat cagttgcccg
    35101 gcggtcatca gggcgttaac gtcgcgtccc tgacacagac cgcgccactg cgcgagagct
    35161 gattcgctac gaccatcgaa ccagttcagc caccacagag tggcgttcag cggataatcg
    35221 cgcagcagtt tctcgcgctg caaacgcgcg ttgtcctgac gaccacttaa caccagcagc
    35281 agattatgca ggcaaagcac ttcctgattg gttgggcagg cgcgaagact ttgttggcaa
    35341 aaatccagac ccgcgtcgaa gttaccatta cgcgccgcca gtcgtgccag accataatag
    35401 cctccggctt tactgttgcc gctccagacc gcacgccaga aatcctcttc ggcttgttga
    35461 tattgtccct gacgttcgta agcactggcg cgaatcaaac tggcctgtcc gcactgcgga
    35521 tttttgttca gcgcatgtgc gcgtttcagc gcctgactgg catacgccac cgcttgcggg
    35581 aaatctgcgc ggttatattc cagcatcgcc agcgccagat tacagcggta atccagcgaa
    35641 tccagcgcca cgccgcgcag gtagtaatcg aacggtgaac ggctggcgtg atgatattgc
    35701 tccagatgct gaccgataaa ccaggcttca tctgtactgg taatgtcttg tgctgacagt
    35761 ggcgctttgg cgacgtccgg caacggcaac tcttgcggct gatgttcctg ataactcagt
    35821 acaatattgc cgtcggcatc ggagagctca atggtcagcc tttcaggatt gataccgtgc
    35881 aacacgccct ggatggcggt cgctggtgtc agggccacgg catcatcaag taacgcgttg
    35941 catttgccga tttcgcggat cgccaggcgg tatccgttca acggagagat ggcatacagc
    36001 ccccactcaa tcccccgctc actacgctgg agttttatca ccgcatcgcg ggaggcattt
    36061 tgcaccatgc ccagagaatg ataaggcagg aaatactgct caaaacgctt ctcttcgtaa
    36121 gcatcaagcc aggtaaaatc gggctggtta tcggcaaaaa taccggtcat cagttcgata
    36181 tacgggccgt tattatcagt taggctctta tcccacgcct ggccaaattc actgtgtccc
    36241 cagctccact gctttttacc tggcgcaata tggtggttgg caacgtgtag caaaccgcca
    36301 tcttcatcgt gacaccacgc gccaacaaaa tcgtactgcg atttttcggc catatatgag
    36361 gttggaacgg gcacattttt atagcgagaa atgtccactc cagcggagta gtccactttg
    36421 tagtaagtgc cggtggcgat ggggaaagcg gagacggccc gtttgccgtg atcaaacacc
    36481 gcagtcacat ccggcgggaa aacgctctga tgcccttccc cccctttcac tgccgggttg
    36541 gcccaccaca agaaatgacg cggcgtggcg ttgccgttat agacgcggct ggcgatttcc
    36601 agcgccgccc ggtcagggcg cagggtgaaa cctgtcatca cctgtaaacc atgcatcggc
    36661 tccgtttcgc ctacccacac cgtctgtgcg ccgtcttcat gggcttcgag ggtgaaatca
    36721 acgggcataa aggtggtcgg gcgatggtgt tgcggccagt taaactcaat cccgccggag
    36781 atccacggtc ccagcagccc caccagcgca ggtttaatga cttcattgtg atagacaaaa
    36841 tcgcgttgtt tcactttatc ccatgcgcga tgcacccgac cgcccaattc cggcaggatc
    36901 atcactttga tgtagtcgtt ttccagccac accgcctgcc aggatttcag ggttttctgc
    36961 tcgctcagcg tatcggtcac gccgtaggga taaaccgcgc cggacgatcc ctgataaacg
    37021 cgattttcca ggaacatggg atgtatatcc tgcggcccgg tttcataggt cggtatctca
    37081 acgcgctctt gccacacttt tactggagtc atggtgccct caatattaac aagacatact
    37141 gaattaaaag atttgtggca gtgtattgaa caatctggca atgttttcgc ggaataatca
    37201 cgcaattaac taaacaaggt ttagtgaaga tgagagcctg cattaacaac caacagattc
    37261 gccaccataa caaatgcgtg attctggaac tgctgtaccg gcaaaagcgc gccaataaat
    37321 caacgctggc ccggctggcg caaatttcga ttccggcagt cagtaatatt ttgcaggaac
    37381 tggaaagcga aaaacgggtg gtgaatatcg acgatgaaag ccagacgcgc gggcatagta
    37441 gcggtacatg gctgattgcg ccggaaggtg actggacgct gtgtctgaac gtgacgccca
    37501 ccagtattga gtgtcaggtt gctaatgctt gtttaagtcc gaaaggtgaa tttgagtatt
    37561 tacagattga tgcaccgacg ccgcaggcgc tgctgtccga aatcgaaaaa tgctggcatc
    37621 gccaccgtaa attgtggccg gaccgcacca tcaatctggc gctggcaatc cacggtcagg
    37681 ttgatccagt gacaggagtg tcgcaaacca tgccgcaagc gccgtggaca acgccggttg
    37741 aggtgaagta tctgctggaa gagaagctcg gcattcgggt gatggtcgat aatgactgcg
    37801 tgatgctggc gctggcggaa aaatggcaaa ataattcgca ggaacgggat ttctgcgtga
    37861 tcaacgttga ttacggcatt ggctcgtcgt tcgtgattaa cgagcaaatt tatcgcggca
    37921 gtttgtatgg tagcggacag attggtcaca ccatcgttaa tccggatggc gtcgtctgcg
    37981 actgtggacg ttacggctgc ctggaaaccg tcgcctcgtt aagtgcttta aaaaaacagg
    38041 cgcgggtatg gctaaaatca caaccggtta atactcagct tgatcctgaa aaactgacaa
    38101 ccgcgcagtt aatcgctgcc tggcagagtg gagaaccgtg gatcaccagc tgggttgacc
    38161 gcagtgccaa tgccattggt ttgagtctgt ataacttcct caacatcctc aatattaatc
    38221 agatttggtt gtacggtcgc agttgtgcct ttggtgagaa ctggcttaat actattattc
    38281 gccagacagg atttaacccg ttcgaccgcg acgaaggacc gagcgtgaaa gcgacgcaaa
    38341 ttggcttcgg gcaattaagc cgcgcacaac aggtgctggg aattggctat ttgtatgttg
    38401 aggcgcagtt acgacagatt tgatggcgcg ataacgtaga aaggcttccc gaaggaagcc
    38461 tttttcaaca gattaaccgt ttcgcttatg cgtaaaccgg gtaacgtgcg cagatgtcga
    38521 gaactttacc tttgatgcgc tcgataacgg cttcatcatt gatgctgtcc agcacgtcac
    38581 acatccagcc agccagttct ttcgcttctg cttctttgaa gccgcgacgc gtaatcgccg
    38641 gagtacccac gcgaataccg gaggtcacaa acgggctctt cggatcgttc ggtacgctgt
    38701 ttttgttgac ggtgatgtta gcacggccca gagcggcgtc tgcttcttta ccggtcaggt
    38761 ttttatcgac cagatcaacc aggaacagat ggttatcagt accgccggaa accactttgt
    38821 agccgcgttc caggaacact tctaccatcg ctttagcgtt tttagcgacc tgctgctggt
    38881 aagttttgaa ctcaggctcc atcgcttctt tcagagcaac cgctttacca gcgattacgt
    38941 gcatcaacgg accgccctga ccaccaggga aaacggcaga gttcagtttt ttgtacagct
    39001 cttcgctacc acctttcgcc aggatcagac cgccgcgcgg acccgccagg gttttgtgag
    39061 tggtggtagt aacaacgtga gcatgaggaa ccgggttcgg gtagacgcca gcagcaacca
    39121 ggcccgcaac gtgcgccata tcaacgaaca ggtaagcacc gatgctgtca gcgatttcac
    39181 gcattttcgc ccagtcaacc acgccggaat atgcagagaa accaccgata atcattttcg
    39241 gcttgtgttc tttggcttgt ttttccagat cggcgtagtc gatatgaccg gtagcatcga
    39301 taccgtaagg aacgatgttg tacagtttac cggagaagtt aaccggagaa ccgtgagtca
    39361 ggtgaccgcc atgcgccagg ttcataccca gaacggtatc acctggttcc agcagcgcgg
    39421 tgtacaccgc aaagttagcc tgggagccgg agtgcggctg gacgttagcg tagtcagcgc
    39481 cgaacagttc tttcgcacga tcgatcgcca gttgttcaac gatatcaaca tactcgcaac
    39541 cgccgtagta gcgtttgccc ggataacctt cagcatattt gttggtcagc tgagaaccct
    39601 gcgcctgcat tacgcgcggg ctggtgtagt tttcggaggc gatcagttcg atgtgctctt
    39661 cctgacgtac tttttcctgc tccatagcct gccacagttc ggcatcataa tcggcaatgt
    39721 tcatttcacg ctttaacatc cgcatctcct gactcagcta acaataaaat ttttggcctt
    39781 tataggcggt cctgttggac aacggcgaac agtataaccg aatcattgtg cgataacagg
    39841 tcttgacaaa ggaatttacg caaacgatta ccttcaggct acgcaaggct ttggagaata
    39901 aagagcttgc aaccggaaac ggatttcttt tcaggtttgt gatgcaaatt tttcacttca
    39961 tcacattctt tctgaaaaac accaaagaac catttacatt gcagggctat tttttataag
    40021 atgcatttga gatacatcaa ttaagatgca aaaaaaggaa gaccatatgc ttgacgctca
    40081 aaccatcgct acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac
    40141 cgcccatttc tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat
    40201 gagtaaccag cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc
    40261 cagtaatatt gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca
    40321 caccagtttc cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac
    40381 gctggacgaa atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg
    40441 tgtactggct aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa
    40501 agccggtggt tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc
    40561 gcttatcacc agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc
    40621 ggggcaatat ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca
    40681 gtactctttg actcgcaaac cggatggcaa aggctatcgt attgcggtga aacgcgaaga
    40741 gggtgggcag gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact
    40801 ggtcgctccg gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat
    40861 ctctgccggt gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg
    40921 ccacacagca caagtgaact ggttccatgc ggcagaaaat ggcgatgttc acgcctttgc
    40981 cgatgaagtt aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg
    41041 tcagccgagc gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt
    41101 gagcaaactg gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt
    41161 tggcttcatg cagtttgccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat
    41221 tcattacgaa tgctttggcc cgcataaggt gctgtgattt gatgttgccg gatggaaaca
    41281 tccggcaacc cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg
    41341 aatgacccgt gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc
    41401 agtgcgaata atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat
    41461 tttcactttc ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc
    41521 tttctggcgg ccaaaacctt tcacctcagt gaccgtcatg ccggtaatgc cgacttcagc
    41581 cagtgcttcg cggacatcgt ccagcttgaa gggtttaata atcgcatcaa tctttttcat
    41641 gctattcctt gaaaaggtcg cctgtctttt gatctgctaa acgtaacaca taacgccaat
    41701 tcattccttg aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt
    41761 ccggttgcgc cccgccattc tcgccgcatg ggtgacgttg cctttggtaa tttgcagcag
    41821 cttacgcaaa tagttgagtt caaactgatt acgtgcctca acaaaggttg gcagcgccgt
    41881 gttttcgccc tccagcgcct gctctactaa tgcatcacta atcaccggag atgaggtcag
    41941 cgccacgcac tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt
    42001 catcaggcgt ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc
    42061 cgcctggcgc aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ctgccagagc
    42121 cggaattttc aggctgacaa cgttaaggcg gtaatagaga tcttcacgga attccccgcg
    42181 caccatcgct tttggcaggt cgcggtgagt ggcggaaata atccgcacgt cgatatcaat
    42241 atcgcggtta ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac
    42301 ctgcaacggt gcgggcatat cgccaatctc atcgagaaat agcgtaccgc cttccgccgc
    42361 ctggaataaa ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa
    42421 cagctccgac tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt
    42481 gctattgcgc gggctggcgt tgtggatagc ctgggcgaac atctctttcc cggtgccgct
    42541 ctgaccgtta atcaaaacgc taacgtctga ttgcgccacc agccgcgcct gttccagcaa
    42601 acgcagcatc agcgggctgc gggtgacaat cgcctcgcgc cagcgttcgt cggtggctgg
    42661 cgcggattgc tccagcgcat catcaattgc ctgatatagc gcgtctttat cgacaggctt
    42721 ggtgaggaaa ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggaa tagaaccatg
    42781 cgcggtaaga ataattaccg gcattcccgg ctgcactttc tggatttcag caaacagctg
    42841 cataccgtcc atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt
    42901 cagtacccgt aaaccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt
    42961 caggcgcagg ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc
    43021 aggtttatgg ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt
    43081 gtggcgtatc cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat
    43141 cagtcaggtt ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt
    43201 gctggcgcag agcgtccagc tcgctgtcgc tggactgctg gagtttactg tagcgttggc
    43261 gctcttccgc cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc
    43321 gaacctgtgc cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct
    43381 catacggcgt aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc
    43441 tgccgtcgtc atattggcgg gcttgctgac gcgactgtgc aggcattaaa cgatcagcac
    43501 aatccatcgc ccgcagccag taaagcggat tggtttcagt tgatttacct tgcagcgccc
    43561 agatgtcgct acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt
    43621 ctgctggcgt atcaatggct ggcttattgt gactctgcac gcaacccagc aatgccagac
    43681 atggcagccc tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat
    43741 tcaccagact taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac
    43801 agcgcggaga aaccggagcg tacatgtcgt acgtgaggat ttcgagcact gcccggggcc
    43861 gaactgacaa ataaaatagc ctgatgggat agactgttag cattatttcg tgtttttcga
    43921 cgacggtaat tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc
    43981 cccttgcata cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac
    44041 cgcccctttt cgctggtggc ttccctgaaa aaagggttcg aagatcatgg cgcgttcctc
    44101 ttgcggaatg ggcgtgcctg tattgatgac atcaatataa acccgcgcgc catgtaaact
    44161 gctgcgaagg caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt
    44221 atccagtacg ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac
    44281 gtcggtatgc atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac
    44341 cagcggtgct aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta
    44401 atcaagcagt tgttcgatca gtttttgcaa attgcggctg ctgctatcaa gaatgctcac
    44461 cacctctttt tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc
    44521 ctcgcgcata ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg
    44581 gcgttgggat tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga
    44641 gcgtaactcg ctcggtccac tgaacgaaac gctattgccc agagaacgcc cttcccccaa
    44701 ccgattgatc atccgttcaa tatttttcac cggcccgata atcatccgcg tgaaaagtag
    44761 caccatcacc agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg
    44821 ttcggcgatt tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg
    44881 caccatttcg gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg
    44941 accactgttg ttgcactgaa gttgggccag attgttcaag tcctgacgca atgcctggta
    45001 aagtttatcg tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg
    45061 ctggctttga taaaccttcg caagcgtagg gtcatccagc acgcaatact gacggtagct
    45121 acgctccatc tccagcgccg cgttggtcat tgcttcgctg cgccgggcat cgataagcgt
    45181 ggtgcggttg accagcgccg cctggtcgct aagcgcattc aggctttgcc aggcttgcca
    45241 tgccagcacc aacaggggca gcagaatcag caaaaatgcc agcattacca gttgtcgtaa
    45301 tgagcgggga aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct
    45361 gatgctaacc gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg
    45421 gaataaggcg gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc
    45481 agaagttgga cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga
    45541 aggggctgac ataagtcggt gaatgagcca ctgattacta ttatgcagta actgtgccaa
    45601 taaagaaaat agtttggtaa cgtactgatt atacgttgct ttgaggggtt tatggcttcc
    45661 ccgctatttg aatgtaaatc agccactgtg tctctaaaaa gagacaactt aagaaaaact
    45721 tattagatta caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac
    45781 ccattgtcgt gatttacaga cacaaaaaag ctcccgaagt tgggagctta taatagcggt
    45841 tgtatttaat gccggatgcg gcgtgaatgc ctcatccggg ctgcaaaacc aatgagtttg
    45901 acggcttacc ccaactgctt acgtgcattg cggaaaatgc gcatccatgg gccatcctcg
    45961 ccccagtttt caggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc
    46021 atcataatgg tgactcgacc gctttcagtc gtgaccgccg taataccgtt cggagaaccg
    46081 ttcgggttag ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc
    46141 accagccctt tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct
    46201 tcaccatgag agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagcgac
    46261 gggctttggg tcacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtattgcgc
    46321 acaaaacgtg gccacaactc actacctggg atcagttcac gcagattaga catcatctgg
    46381 caaccgttac atacccccag cgccagcgtt tgcggacggt ggaagaaggt tgcaaactca
    46441 tcgcgtacac ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca
    46501 tcaccgtagg agaaaccacc gcacgcgacc agggcgtgga aatcctccag gcccgtgcgt
    46561 ccagccagca ggtcgctcat atgcacgtcg atagcatcga agcctgcacg gtggaaagct
    46621 gccgccattt caacatgcga gttcacgccc tgctcacgca gcacagcaac tttcggacgt
    46681 gcgccagtgg caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca
    46741 ttcaggcccg gatcggcgtc gtttgatttc gcctgatgct cctgatcggc acactccggg
    46801 ttgtcacgca ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg
    46861 cggctttcgc tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc
    46921 gcctgcccaa cataatggac gcaatcagca agcccatgct gtgccagtac ggactcgacc
    46981 gcttcacggt cagcggcacg aacctgaatg accgcaccca gttcttcgtt aaacaacgcc
    47041 gccaggcgat cgtcacccag agtggcgata tccgcgtaaa tgccgcaatg accagcaaag
    47101 gccatttccg ccagcgttac cagcagaccg ccatcagagc ggtcgtgata cgccagcagc
    47161 ttacgctgtg caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg cgcgacatcg
    47221 cgtacatctg ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc
    47281 agcgcgttat tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtagaaagc
    47341 tgcggcgtga tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac
    47401 agcggcgacg tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata
    47461 gagtctttac ccaccgggat cgtcaggccc agcgccggac aaagctcttc gcccacggct
    47521 ttaacggctt catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg
    47581 gcggaaagtt taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct
    47641 tcaccgaccg ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg
    47701 ccaatcgcca tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta
    47761 gcgaccggca cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg
    47821 cggtcaccga tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt
    47881 ttcaccgcgt ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc
    47941 gtttgtacat cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg
    48001 atcggctgat tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca
    48061 ccaatcaccg cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaactga
    48121 tcggcagcaa ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg
    48181 ctcatgcccg gttcgtcgct caggatatcg cgcagttcaa atttaccgcc gcgcccgccg
    48241 tcgcttacca gttccggcat cgcgttagaa agaccgcctg caccaacgtc gtggataaac
    48301 aggattgggt tggcatcacc aagctgccag caacgatcga tcacttcctg gcagcgacgc
    48361 tccatttccg ggttgtcgcg ctggacggaa gcaaagtcga ggtcggcatc agactgacct
    48421 gacgccatgg aagacgccgc tccgccgcca agaccgatgt tcattgccgg gccgccgaga
    48481 acgaccagct tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg
    48541 ccgatcccgc ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccgttgtgg
    48601 ctgttcactt tttcttcata agtacggaag tagccattca gtgccggacg accaaattcg
    48661 ttgttaaacg ccgcgccgcc cagcgggcct tcggtcatga tgtccagcgc ggtgacaatg
    48721 cgctcaggct taccgaaatc ttcttcccac ggctgttcga agcccggaat acgcaggttg
    48781 gaaacggaga aaccgaccag accagctttc ggcttcgcgc cacgcccggt ggcgccttca
    48841 tcgcggattt caccgccgga accggtcgcc gcgcccggcc acggtgaaat cgccgtcggg
    48901 tggttgtggg tttcaacttt catcaggata tgcaccggtt cctgatggaa atcgtagcgg
    48961 cccgtttcgt ggtcggcaaa ataacggccc acttcagaac cttccattac ggcggcgtta
    49021 tctttataag cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg
    49081 aacagcgatt tcggctgctg ttcaccgtcg ataatccagt cggcattaaa aattttgtgg
    49141 cggcagtgct cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga
    49201 ccaagctttg tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaggcca
    49261 agacgcaggt tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgctggta
    49321 accggagtcg gttgatggtg ggcaaacaac tgctctgcat catctaaagc aaaaaagacc
    49381 gtttccatca tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc
    49441 gtaccggctt ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg
    49501 cagttatggg caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg
    49561 gtcaccagca ggagtttgcc ttgcggggcg tggctggcga gtgccgggcc atatttcagc
    49621 aggcgttcaa gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg
    49681 acatactcgg cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt
    49741 ttgttgattc ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc
    49801 tcgtcttcta agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct
    49861 gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt
    49921 gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt
    49981 tatgagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag
    50041 aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct
    50101 gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc
    50161 cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa
    50221 cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta
    50281 cctcggtgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct
    50341 cgataatggt aacgccgacc tgctggcggc aggacttgtc tataacagtg agcgggtaaa
    50401 aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg
    50461 tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg
    50521 tcatgtggtg gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg
    50581 gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact
    50641 cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct
    50701 cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg
    50761 cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac
    50821 gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac
    50881 gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa
    50941 atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg
    51001 ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac
    51061 cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt
    51121 gcgttatttg cagcatatga tgagtaaagt gccggaaagt gtgccggaga acgagcggat
    51181 ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct
    51241 gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt
    51301 acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc
    51361 ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga
    51421 gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc
    51481 gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc
    51541 aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca
    51601 aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg
    51661 aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac
    51721 tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt
    51781 cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca
    51841 cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat
    51901 caccagaccg ccctgcctca gggccatgat ttctgcatgt gcggtgggat catgtcgacc
    51961 aatcggacgg ttccagcctt cgccaataac acgattgtta tgcactaata ccgcgccgac
    52021 cggtacttcc cgctcatccc aggcacgctt cgccagcgtc attgcatgac gcatccagta
    52081 ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat
    52141 tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac
    52201 tcgccagcga tgctggcaaa agtaaagcag cgggttgtcc tgattactgt cgctatagcc
    52261 actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgcgcgacct tttcatgtcc
    52321 cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag
    52381 attaactcgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga
    52441 gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg
    52501 aaccagcgga aaggcggtaa cattgtcacg aaaccagcgt acgaaatcgg cctgcaacgc
    52561 ctgcaaacgc gcttcattgt gaccaaaagt gcatccccac agaagcagac tcatcggcca
    52621 gcgtgccgca cgacctttta tcaataacgc aatggctata atcggcaaca acggcaggac
    52681 aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc
    52741 ctgctgatgc aatgttccat ctaaatcaaa aaacaccaca cgacgctcgt gagttgccaa
    52801 accatactcc tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga
    52861 tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc
    52921 atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg
    52981 tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta
    53041 ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac
    53101 ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt
    53161 cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag
    53221 tgaagcgctg gcaagccagc cggaatcgcc ctcagtgccc attcataacc aaatccgcgg
    53281 tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta
    53341 cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc
    53401 tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc
    53461 ctggaagctg atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc atgcgctact
    53521 cgctaccgta caggcgtcgt cccctgacga tctgttatta gccatttctt acaccggtgt
    53581 acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc
    53641 gattaccggc tttactccca atgccctgca acagcgtgct tctcattgcc tgtataccat
    53701 tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgcgc agggaatgtt
    53761 aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat
    53821 tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc
    53881 cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca
    53941 tcaattgtga tatgtgtgaa atcatataaa aacacattta ttttcatagt gatacagtaa
    54001 tgtgttattg aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa
    54061 cacaaagcct ccccctttct ttagcatttt ctactgagca gccatccgct ttcagacctc
    54121 acttaaatta cgcttattca gagaagagcg tcagaaacac gatcggcatc actgtttctg
    54181 caaact
//
LOCUS       DAZGBC010000012        53130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGBC010000012 DAZGBC010000000
VERSION     DAZGBC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.012"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..518
                     /locus_tag="ACHK1J_000749"
     CDS             <1..518
                     /locus_tag="ACHK1J_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7910073.1"
                     /translation="FCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIG
                     RFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIF
                     SLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFI
                     FARFRSQTATN"
     gene            551..2326
                     /gene="fucI"
                     /locus_tag="ACHK1J_000750"
     CDS             551..2326
                     /gene="fucI"
                     /locus_tag="ACHK1J_000750"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO7910074.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            2435..3853
                     /gene="fucK"
                     /locus_tag="ACHK1J_000751"
     CDS             2435..3853
                     /gene="fucK"
                     /locus_tag="ACHK1J_000751"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO7910075.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGIGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            3855..4277
                     /gene="fucU"
                     /locus_tag="ACHK1J_000752"
     CDS             3855..4277
                     /gene="fucU"
                     /locus_tag="ACHK1J_000752"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO7910076.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            4335..5066
                     /gene="fucR"
                     /locus_tag="ACHK1J_000753"
     CDS             4335..5066
                     /gene="fucR"
                     /locus_tag="ACHK1J_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO7910077.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(5110..6210)
                     /gene="rlmM"
                     /locus_tag="ACHK1J_000754"
     CDS             complement(5110..6210)
                     /gene="rlmM"
                     /locus_tag="ACHK1J_000754"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO7910078.1"
                     /translation="MNKVVLLCRPGFEKECAAEISDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(6203..6598)
                     /gene="ygdD"
                     /locus_tag="ACHK1J_000755"
     CDS             complement(6203..6598)
                     /gene="ygdD"
                     /locus_tag="ACHK1J_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO7910079.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(6617..7534)
                     /gene="gcvA"
                     /locus_tag="ACHK1J_000756"
     CDS             complement(6617..7534)
                     /gene="gcvA"
                     /locus_tag="ACHK1J_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO7910080.1"
                     /translation="MYKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(7885..8112)
                     /gene="ygdI"
                     /locus_tag="ACHK1J_000757"
     CDS             complement(7885..8112)
                     /gene="ygdI"
                     /locus_tag="ACHK1J_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO7910081.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            8304..9509
                     /gene="csdA"
                     /locus_tag="ACHK1J_000758"
     CDS             8304..9509
                     /gene="csdA"
                     /locus_tag="ACHK1J_000758"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO7910082.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            9509..9952
                     /gene="csdE"
                     /locus_tag="ACHK1J_000759"
     CDS             9509..9952
                     /gene="csdE"
                     /locus_tag="ACHK1J_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO7910083.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRKTFAPLTQWEEKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAEHGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(10003..10809)
                     /gene="tcdA"
                     /locus_tag="ACHK1J_000760"
     CDS             complement(10003..10809)
                     /gene="tcdA"
                     /locus_tag="ACHK1J_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO7910084.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(11047..12144)
                     /gene="mltA"
                     /locus_tag="ACHK1J_000761"
     CDS             complement(11047..12144)
                     /gene="mltA"
                     /locus_tag="ACHK1J_000761"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO7910085.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            12353..12429
                     /locus_tag="ACHK1J_000762"
     tRNA            12353..12429
                     /locus_tag="ACHK1J_000762"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12387..12389,aa:Met,seq:cat)
     gene            complement(12503..13756)
                     /gene="amiC"
                     /locus_tag="ACHK1J_000763"
     CDS             complement(12503..13756)
                     /gene="amiC"
                     /locus_tag="ACHK1J_000763"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO7910086.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            13988..15319
                     /gene="argA"
                     /locus_tag="ACHK1J_000764"
     CDS             13988..15319
                     /gene="argA"
                     /locus_tag="ACHK1J_000764"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO7910087.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(15381..17207)
                     /gene="recD"
                     /locus_tag="ACHK1J_000765"
     CDS             complement(15381..17207)
                     /gene="recD"
                     /locus_tag="ACHK1J_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO7910088.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(17207..20749)
                     /gene="recB"
                     /locus_tag="ACHK1J_000766"
     CDS             complement(17207..20749)
                     /gene="recB"
                     /locus_tag="ACHK1J_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO7910089.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESRLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLSDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYSLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAELITLMDEMFAGMTLEEA"
     gene            complement(20742..23630)
                     /gene="ptrA"
                     /locus_tag="ACHK1J_000767"
     CDS             complement(20742..23630)
                     /gene="ptrA"
                     /locus_tag="ACHK1J_000767"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO7910090.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAASIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(23806..27174)
                     /gene="recC"
                     /locus_tag="ACHK1J_000768"
     CDS             complement(23806..27174)
                     /gene="recC"
                     /locus_tag="ACHK1J_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO7910091.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(27187..27510)
                     /gene="ppdC"
                     /locus_tag="ACHK1J_000769"
     CDS             complement(27187..27510)
                     /gene="ppdC"
                     /locus_tag="ACHK1J_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO7910092.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(27495..27902)
                     /gene="ygdB"
                     /locus_tag="ACHK1J_000770"
     CDS             complement(27495..27902)
                     /gene="ygdB"
                     /locus_tag="ACHK1J_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO7910093.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMYSWQTQPAVQCLQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRAGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(27899..28462)
                     /gene="ppdB"
                     /locus_tag="ACHK1J_000771"
     CDS             complement(27899..28462)
                     /gene="ppdB"
                     /locus_tag="ACHK1J_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7910094.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(28453..28923)
                     /gene="ppdA"
                     /locus_tag="ACHK1J_000772"
     CDS             complement(28453..28923)
                     /gene="ppdA"
                     /locus_tag="ACHK1J_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7910095.1"
                     /translation="MKTQRGYTLIETLVAMLILVTLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(29108..29902)
                     /gene="thyA"
                     /locus_tag="ACHK1J_000773"
     CDS             complement(29108..29902)
                     /gene="thyA"
                     /locus_tag="ACHK1J_000773"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO7910096.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(29909..30784)
                     /gene="lgt"
                     /locus_tag="ACHK1J_000774"
     CDS             complement(29909..30784)
                     /gene="lgt"
                     /locus_tag="ACHK1J_000774"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO7910097.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(30935..33181)
                     /gene="ptsP"
                     /locus_tag="ACHK1J_000775"
     CDS             complement(30935..33181)
                     /gene="ptsP"
                     /locus_tag="ACHK1J_000775"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7910098.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(33194..33724)
                     /gene="rppH"
                     /locus_tag="ACHK1J_000776"
     CDS             complement(33194..33724)
                     /gene="rppH"
                     /locus_tag="ACHK1J_000776"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO7910099.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(34077..34223)
                     /gene="ygdT"
                     /locus_tag="ACHK1J_000777"
     CDS             complement(34077..34223)
                     /gene="ygdT"
                     /locus_tag="ACHK1J_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO7910100.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFGGSVLNLVAHSVDK
                     YGIG"
     gene            34409..35098
                     /gene="mutH"
                     /locus_tag="ACHK1J_000778"
     CDS             34409..35098
                     /gene="mutH"
                     /locus_tag="ACHK1J_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO7910101.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            35167..35880
                     /gene="ygdQ"
                     /locus_tag="ACHK1J_000779"
     CDS             35167..35880
                     /gene="ygdQ"
                     /locus_tag="ACHK1J_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7910102.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            36018..36236
                     /gene="ygdR"
                     /locus_tag="ACHK1J_000780"
     CDS             36018..36236
                     /gene="ygdR"
                     /locus_tag="ACHK1J_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO7910103.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            36344..37384
                     /gene="tas"
                     /locus_tag="ACHK1J_000781"
     CDS             36344..37384
                     /gene="tas"
                     /locus_tag="ACHK1J_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO7910104.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(37416..38609)
                     /gene="lplT"
                     /locus_tag="ACHK1J_000782"
     CDS             complement(37416..38609)
                     /gene="lplT"
                     /locus_tag="ACHK1J_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO7910105.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(38602..40761)
                     /gene="aas"
                     /locus_tag="ACHK1J_000783"
     CDS             complement(38602..40761)
                     /gene="aas"
                     /locus_tag="ACHK1J_000783"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO7910106.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            41347..42378
                     /gene="galR"
                     /locus_tag="ACHK1J_000784"
     CDS             41347..42378
                     /gene="galR"
                     /locus_tag="ACHK1J_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO7910107.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(42385..43647)
                     /gene="lysA"
                     /locus_tag="ACHK1J_000785"
     CDS             complement(42385..43647)
                     /gene="lysA"
                     /locus_tag="ACHK1J_000785"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO7910108.1"
                     /translation="MPHSLFSTDTALTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            43769..44704
                     /gene="lysR"
                     /locus_tag="ACHK1J_000786"
     CDS             43769..44704
                     /gene="lysR"
                     /locus_tag="ACHK1J_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO7910109.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(44691..45383)
                     /gene="ygeA"
                     /locus_tag="ACHK1J_000787"
     CDS             complement(44691..45383)
                     /gene="ygeA"
                     /locus_tag="ACHK1J_000787"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO7910110.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEELSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(45512..46930)
                     /gene="araE"
                     /locus_tag="ACHK1J_000788"
     CDS             complement(45512..46930)
                     /gene="araE"
                     /locus_tag="ACHK1J_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO7910111.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(47245..48006)
                     /gene="kduD"
                     /locus_tag="ACHK1J_000789"
     CDS             complement(47245..48006)
                     /gene="kduD"
                     /locus_tag="ACHK1J_000789"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO7910112.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(48036..48872)
                     /gene="kduI"
                     /locus_tag="ACHK1J_000790"
     CDS             complement(48036..48872)
                     /gene="kduI"
                     /locus_tag="ACHK1J_000790"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO7910113.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVTDEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASDDTATPAKFYYNCVPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(49159..50340)
                     /gene="yqeF"
                     /locus_tag="ACHK1J_000791"
     CDS             complement(49159..50340)
                     /gene="yqeF"
                     /locus_tag="ACHK1J_000791"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO7910114.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVIDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            50595..51824
                     /gene="yqeG"
                     /locus_tag="ACHK1J_000792"
     CDS             50595..51824
                     /gene="yqeG"
                     /locus_tag="ACHK1J_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7910115.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            52284..52916
                     /gene="yqeH"
                     /locus_tag="ACHK1J_000793"
     CDS             52284..52916
                     /gene="yqeH"
                     /locus_tag="ACHK1J_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7910116.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT        13213 a        14259 c        12960 g        12698 t
ORIGIN      
        1 aattctgcta cgttggcgca caaacggcct gctggagcta tttgattcgc tacgctgtag
       61 aagaaattcc aggtatgact gcaggctttg ccgctaacta tttaaccgga accatggtgt
      121 gcttctttat tggtcgtttc accggtacct ggctcatcag tcgcttcgca ccacacaaag
      181 tcctggccgc ctacgcatta atcgctatgg cactgtgcct gatctcagcc ttcgctggcg
      241 gtcatgtggg cttaatagcc ctgactttat gcagcgcctt tatgtcgatt cagtacccaa
      301 caatcttctc gctgggcatt aagaatctcg gccaggacac caaatacggt tcgtccttca
      361 tcgttatgac catcattggc ggcggtattg tcactccggt catgggtttt gtcagtgacg
      421 cggcgggcaa catccccact gctgaactga tccccgcact ctgcttcgcg gtcatcttta
      481 tctttgcccg tttccgttct caaacggcaa ctaactgaac atattttccg aataaagtga
      541 ggaatctgta atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg ttattgacgg
      601 tcgtcgcatg ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg cgaaagctac
      661 ggccgcactg ctgaccgaga aactgcgcca tgcctgcgga gctgccgtcg agtgtgtcat
      721 ttccgatacc tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa aattcagcag
      781 tcagaatgta ggcctcacca ttacggtaac gccttgctgg tgctatggca gtgaaaccat
      841 cgacatggat ccaacccgcc cgaaagccat ttggggcttt aacggcactg aacgtcccgg
      901 cgctgtttac ctggcagcgg ctctggcagc tcacagccag aaaggcatcc cggcattctc
      961 catttacggt catgacgttc aggatgccga tgacacatcg attcctgccg atgttgaaga
     1021 aaaactgctg cgctttgccc gcgccggttt ggccgtcgcc agcatgaaag gtaaaagcta
     1081 tctgtcgctg ggcggcgttt cgatgggtat cgccggttct attgttgatc acaacttctt
     1141 tgaatcctgg ctgggaatga aagtccaggc ggtggatatg accgaactgc gtcgccgtat
     1201 cgatcagaag atttacgacg aagccgaact ggaaatggca ctggcctggg ctgataaaaa
     1261 cttccgctat ggcgaagatg aaaataacaa acagtatcaa cgtaatgccg agcaaagccg
     1321 cgcagttctg cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga tgcaaggcaa
     1381 cagcaaactg gccgatattg gtcgcgtgga agaatcactt ggctacaacg ccatcgctgc
     1441 gggcttccag gggcaacgtc actggaccga tcaatatccc aatggtgaca ccgccgaagc
     1501 gatcctcaac agttcatttg actggaatgg cgtgcgcgaa ccctttgtcg tggcgaccga
     1561 aaacgacagt cttaacggcg tggcaatgct aatgggtcac cagctcaccg gcaccgctca
     1621 ggtatttgcc gatgtgcgta cctactggtc accagaagca attgagcgtg taacggggca
     1681 taaactcgat ggactggcag aacacggcat catccatttg atcaactcag gttctgctgc
     1741 gctggacggt tcctgtaaac aacgcgacag cgaaggtaac ccgacgatga agccgcactg
     1801 ggaaatctct cagcaagagg ctgacgcttg cctcgccgct accgaatggt gcccggcgat
     1861 ccacgaatac ttccgtggcg gcggttactc ttcccgcttc cttaccgaag gcggcgtccc
     1921 gttcaccatg actcgtgtca acatcatcaa aggcctggga ccggtactgc aaatcgcgga
     1981 aggctggagc gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac gcaccaactc
     2041 aacctggcca accacctggt ttgcaccgcg cctcaccggt aaagggccgt ttacggatgt
     2101 gtactcggta atggcgaact ggggcgctaa ccatggggtt ctgaccatcg gccacgttgg
     2161 cgcagacttt atcactctcg cctccatgct gcgtatcccg gtatgtatgc acaacgttga
     2221 agagaccaaa gtgtatcgtc cttctgcctg ggctgcgcac ggcatggata ttgaaggcca
     2281 ggattaccgc gcttgccaga actacggtcc gttgtacaag cgttaataca ttactccctg
     2341 atgtgatgcc cggtcgctcc ggctaccggg cctgaacaag caagagtggt tagccggata
     2401 agcaatgtta tccggctata ttgcaggagc gattatgaaa caagaagtta tcctggtact
     2461 cgactgtggc gcgaccaatg tcagggccat cgcggttaat cggcagggaa aaattgttgc
     2521 ccgcgcctca acgcctaatg ccagcgatat cgcgatggaa aacaacacct ggcaccagtg
     2581 gtctttagac gccattttgc aacgctttgc tgattgctgt cggcaaatca atagtgaact
     2641 gactgaatgc cacatccgcg gtatcgccgt caccaccttt ggtgtggatg gcgctctggt
     2701 agataagcaa ggcaatctgc tctatccgat cattagctgg aaatgtccgc gaacagcagc
     2761 ggttatggac aatattgaac ggttaatctc cgcacagcgg ttgcaggcta tttctggcgt
     2821 cggagccttt agtttcaata cgttatataa gttggtgtgg ttgaaagaaa atcatccaca
     2881 actgctggaa cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc accgtttaac
     2941 cggcgaattc actactgata tcacgatggc cggaaccagc cagatgctgg atatccagca
     3001 acgcgatttc agtccacaaa ttttacaagc caccggtatt ccacgccgac tcttccctcg
     3061 tctggtggaa gcgggtgaac agattggtac gctacagaac agcgccgcag caatgctcgg
     3121 cttacccgtt ggcataccgg tgatttccgc aggtcacgat acccagttcg ccctttttgg
     3181 cgctggtgct gaacaaaatg aacccgtgct ctcttccggt acatgggaaa ttttaatggt
     3241 tcgcagcgcc caggttgata cttcgctgtt aagtcagtac gccggttcca cctgcgaact
     3301 ggatagccag gcagggttgt ataacccagg tatgcaatgg ctggcatccg gcgtgctgga
     3361 atgggtgaga aaactgttct ggacggctga aacaccctgg caaatgttga ttgaagaggc
     3421 tcgtctgatc gcgcctggcg cggatggcgt gaaaatgcag tgtgatttat tgtcgtgtca
     3481 gaacgctggc tggcaaggag tgacgcttaa taccacgcgg gggcatttct atcgcgcggc
     3541 gctggaaggg ttaactgcgc aattacagcg caatctacag atgctggaaa aaatcgggca
     3601 ctttaaagcc tctgaattat tgttagtcgg tggaggaagt cgcaacacat tgtggaatca
     3661 gattaaagcc aatatgcttg atattccggt aaaagttctc gacgacgccg aaacgaccgt
     3721 cgcaggagct gcgctgttcg gttggtatgg cataggggaa tttaacagcc cggaagaagc
     3781 ccgcgcgcag attcattatc agtaccgtta tttctacccg caaactgaac ctgaatttat
     3841 agaggaagtg tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa ctattgaaag
     3901 tgctggcaga gatgggacat ggagatgaaa ttattttttc cgatgctcac tttcccgccc
     3961 attcgatggg accgcaggtg atccgcgctg atggcctgtt ggtgagcgac ttgctccagg
     4021 cgattatccc gttatttgaa ctggacagtt atgcaccgcc gctggtgatg atggcagcgg
     4081 tagaaggtga cactctcgat cctgaagtag aacgacgtta ccgtaatgcg ctttcactac
     4141 aagccccgtg tcctgacatc atccgcatca atcgttttgc gttttatgaa cgggcgcaaa
     4201 aagcctttgc gatcgttatc acaggcgaac gagcgaagta cgggaatatt cttttaaaaa
     4261 aaggggtaac accgtaatct cataccggta cgcccgcatg acgcgggcgg ttatcgaatg
     4321 atggggtgaa aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc
     4381 ataccagcct gaccacggaa gctctctctg aacagctaaa ggtcagtaaa gaaaccattc
     4441 gtcgcgatct caatgaatta cagacgcagg gtaaaattct gcgcaatcat ggacgcgcta
     4501 aatatatcca ccgtcaaaat caagacagtg gcgatccctt tcacatcagg ctgaaaagcc
     4561 attatgcgca taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg
     4621 tgatagcctt agacgccagt tcaacctgct ggtatctggc acgccagttg cctgacatca
     4681 acattcaggt cttcaccaat agccatccga tttgccatga actcggtaaa cgcgaacgca
     4741 ttcaactgat cagttccggc ggcacacttg agcgcaaata tggctgttac gtcaatccct
     4801 cgctgatttc ccaacttaaa tcgctggaaa tcgatctgtt tattttttct tgtgaaggga
     4861 tcgatagcag cggcgcactg tgggactcca atgccatcaa cgctgattac aaatcgatgc
     4921 tattaaaacg tgccgcgcaa tcgttgttat tgattgataa aagtaaattt aatcgttcag
     4981 gggaagcccg catcgggcat ctggatgagg taacgcacat tatttctgat gagcgccagg
     5041 ttgcaacttc tttggtaaca gcctgacgga aagggtagca ggccggagac gacgccccgg
     5101 ccttgcctgt tatcgctcgt cgcgacgacc acccaccgca gcccagatgc ggcggacgtg
     5161 caccgtcact tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc
     5221 atgttcatca agctgtgcct gaatatacgc cagattgtgt gacacttctt cgtagcgttt
     5281 tttcatcggc agtttgaggt tgaaaatggt ttcacggcac cagccattaa ccagccactg
     5341 cgccatcaat gccgcaactt tcgccggttt ttcaaccata tcgcatacca tccaggagat
     5401 attgctgcgt gtcggacgga atttgaaccc gtcttcccgt agccacgtca cctgcccggt
     5461 atccatcaga ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt
     5521 caccagttgg taggtccagc cgcctgggca agcgcccaaa tccaccgccc acatcccgtt
     5581 cgccaggcgt tcatcccact catccgcagg aataaacaca tgaaatgcct cttccagttt
     5641 gagcgtggaa cgactcggcg catctgccgg aaatttcagg cgcggaatgc ccatatagaa
     5701 cggcgaatta ttgttgctgt atgagtaacc ggtataacag cagcctggtg caatgaagaa
     5761 tacatgcact accggacgct tcggcgtttc atagttcgcc agcacccccg catcgcgcaa
     5821 ggcagcgcgt agcggcacgg tgaatttacg gcagaatttc agcaactctt tgctttcgtt
     5881 ggtatcggca acttcaacac gcagttcacc gcccttctct actacgccct gtaacatgcc
     5941 gacaatgggg gtaatacgat cttctggcgg caaatgctgc aggagttccc ccaccacaaa
     6001 ccactggcgg gcaaaaatta atgaactgaa cggcaactca cggattaact tatcgccatc
     6061 atcaggttga taacattcat aaatgacata acccgcattc tctttcacgc gggcaaaacc
     6121 gaaaatttcc cgctggccgg ctttatcgct aatttctgcg gcgcactctt tttcaaagcc
     6181 cggacggcac agcaatacaa ccttattcat gacttacgcc cttgcgcttt aaacggatag
     6241 caccaactaa cattaacgcc cagcccgcaa ggaagctcac gccgccaacc ggagtgacaa
     6301 acgcccacaa acgcagatgg gacagtgcca ggcaataaag gctgccgctg aacagcaccg
     6361 tgcctaacgc gaggaaaacg ctactccagt aaaaccagat actgatgcga cgctgcattg
     6421 ccaccgccag acctaagatc gccagcgtat gaaacgcctg gtattcgagg ccggtttgga
     6481 tccagcccat ctcaacggcc cccatggttt tacttaacac atgcgcgcca aaagcgccca
     6541 gagccacaaa aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg
     6601 tcgtacccta cgtaaattat tgttcataac gaaagcggaa tttttcttgt tcagcagcgg
     6661 ctttcgccag gatccattgg cgaaaggcgg ctattttacc cagttctgcc tggctgtcat
     6721 gacaaaccag ataaaaagcg tttttactga ccagaacatc attaaacggg caaacaagac
     6781 gtccggcttc aatttcagat tgcgccatca cgttatttgc cagcgccact ccctgcccgt
     6841 gaatagccgc ttgcagcacc atggcgctat ggctaaaaat tggcccttgc tgaacgttga
     6901 tatgatttaa ccccaattga cgagtatatg tctgccagtc acggcgtgac gcatcatgta
     6961 ataacgtatg tttagccaga tcttccgggg tcttcaaggg tttttcgcca gtcagcagta
     7021 gcggcgaaca caccggcaat aaatattcgg cgtacagttt ttccacccgt agccccggcc
     7081 agttgccccg accataaaat atcgccacat caacatcatc cgccagctta tcttcctgac
     7141 gatcaaccgc ctggattcga acgtcaattc ccggataagc tgaattaaag ctggaaagtc
     7201 gcggaaccaa ccaatggatg gcgaaactgg ggagtaaact gaccgtcaac gcccccttgg
     7261 cgctacgggc ctggagttta cgcgtcgctt cggttaattg cgaaaatatc tctttgatat
     7321 cgaggaaata gctttgacct tcctcggtca gcaggagtga acgattacgg cggcggaaca
     7381 gttttagccc cagaaaatcc tcaagagact tgatttgatg acttactgcg gcctgggtca
     7441 caaaaagctc ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca tcaaaaactc
     7501 gtaaggcatt tagcggtggt aatcgtttat acatggcttt taaactttga tgttaaatga
     7561 atttaacaat tagatcacac tgtgtaacct attagttttt ttaatctgag ccattataaa
     7621 ttgtccgttg agcttctacc agcaaatacc tatagtggcg gcacttcctg agccggaacg
     7681 aaaagtttta tcggaatgcg tgttctggtg aacttttggc ttacggttgt gatgttgtgt
     7741 tgttgtgttt gcaattggtc tgcgattcag accatggtag caaagctacc ttttttcact
     7801 tcctgtacat ttaccctgtc tgtccatagt gattaatgta gcaccgccta attgcggtgc
     7861 ttttttttac cttgcgatcg cgaattactg atccagttcg accatctctt tcacgtcggt
     7921 acggttgatc tgctgtttgt tgccattagc gtctttatac gaaatcatac cggtatcgtt
     7981 atcagtctgt ggtttgccgt cagagacgat ggtacgtccg tcattggtgt gcatcacata
     8041 gttcgaaccg gaacaggcgc tcagggcaaa agtcagcata caggcagaaa taattgcggc
     8101 agtctttttc atcatcttct ccttaaagcg attaataaat cttaatagta ttgattaaca
     8161 ggctaaaatt aacgcctaac actattcagc atatgttact gacgcggctt cgccaggata
     8221 tccagataat tctgatggtt agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc
     8281 ggtgcatcaa gccgaggagt accatgaacg tttttaatcc cgcgcagttt cgcgcccagt
     8341 ttcccgcact acaggatgcg ggcgtctatc tcgacagcgc cgcgaccgcg cttaaacctg
     8401 aagccgtggt tgaagccact cgacagtttt acagcctgag cgccggaaac gtccatcgca
     8461 gccagtttgc cgaagcccaa cgcctgaccg cgcgttatga agctgcacga gagaaagtgg
     8521 cgcaattact gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc accactgaat
     8581 ccatcaacat ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc gatgagatta
     8641 ttgtcagcgt ggcagaacac cacgctaacc tcgtcccctg gctgatggtc gcccaacaaa
     8701 ctggagccaa agtggtgaaa ttgccgctta atgcgcagcg actgccggat gtcgatttgt
     8761 tgccagaact gattactccc cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtta
     8821 ctggcggttg cccggatctg gcgcgagcga ttacctttgc tcattcagcc gggatggtgg
     8881 tgatggttga tggtgctcag ggggcggtgc atttccccgc ggatgttcag caactggata
     8941 tcgatttcta tgctttctct ggtcacaaac tgtatggccc gacgggtatc ggcgtgctgt
     9001 atggcaaatc agaactgctg gaagcgatgt cgccctggct aggcggcggc aaaatggttc
     9061 acgaagtgag ttttgacggc ttcacgactc aatctgcgcc gtggaaactg gaagcaggaa
     9121 cgccaaatgt ggctggtgtc ataggattaa gcgcggcgct ggaatggctg gcagattacg
     9181 atatcaacca ggccgaaagc tggagccgta gtttagcaac actggcggaa gatgcactgg
     9241 cgaaacgtcc aggcttccgt tcattccgct gtcaggattc cagcctgctg gcctttgatt
     9301 ttgctggcgt tcatcacagc gatatggtga cgctgctggc ggagtacggt attgccttgc
     9361 gggccgggca acattgcgct cagccgctac tggcagaatt aggcgtaacc ggcacactgc
     9421 gcgcctcttt tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg
     9481 accgcgcgct ggaattattg gtggattaat gacgaacccg caattcgccg gacatccgtt
     9541 cggcacaacc gtaaccgcag aaacgttacg caagaccttc gcaccgttga cgcaatggga
     9601 agaaaaatat cgccagttga tcatgctggg gaaacagctt ccggctttgc cagacgagtt
     9661 aaaagcgcag gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg gatataccgt
     9721 ggctgaacac ggtaaaatgc acttctttgg cgacagcgaa ggacgtatag tgcgcggcct
     9781 gctggcggtg ctgttgactg ccgttgaggg gaaaaccgcc gccgaattgc aggcacagtc
     9841 gccgctggca ttgtttgatg agctgggatt gcgtgcgcaa cttagcgcct cacgcagcca
     9901 ggggctgaat gcgttaagcg aggcgattat cgctgcggcg aagcaggttt aagcacagta
     9961 ccaggtcgga taagacgttc gcgtcgcatc cgacctgatt atttacccct gacgagccgc
    10021 tttcgccatc atctttttca gcgcatgaga aaccgcaaca aaaccaaagg tggcggtcac
    10081 catcgtcgcc gcgccaaatc cagatgcaca atccatccgc ttcggccctt ctgccgtggc
    10141 tttcatagca cacaccgtac cgtctgactg cgggtacacc aatgcttcgg tagagaacac
    10201 gcaatcgacg ccgagcttac ctttactgtt tttcactacg ccaaaatcgc ttttcaggcg
    10261 ctcacgcaac ttcgccgcca gcgggtcctg aatcgttttc gccagatcgg taacctgaat
    10321 ctgcgtcggg tcaatctgcc cacccgcgcc accggttgtg accagtggga ttttattgcg
    10381 ccgacaataa gcaatcagcg ccgctttggg ccgtacacta tcaatggcat caatcacgta
    10441 cgaataaccc acgctcatat actgcgctac gttatccggc gtcacgaaat catccaccac
    10501 cgttacacgg cactccgggt taatctggcg aatacgctcc gccataactt ccgcttttgc
    10561 cagcccgacg ttatcgcgca gagcgtgaat ttgccgattg gtattggtga cgcacacatc
    10621 atccatatcg ataagcgtga ttgcgccaat ccccgtgcgc gccagcgctt ccgccgccca
    10681 ggaaccgaca ccgccaatac cgaccacaca aatatgcgcg tcagcaaaca gttgcagcgc
    10741 tttttcacca tacagacgcg ctgtgccacc aaaacgctga cgccatgcat cactaattac
    10801 cacagacata gcacctcaga ataaaaaggg cgaggttatc cctcacccta aacgcataaa
    10861 tgcctgatgc gctacgctta tcaggcctac ataactcctg caatattttg gatttgcatg
    10921 atttgtaggc cggataaggc gttcacgccg catccggcat gaacaacgcg cacttagtca
    10981 ataatcagga tgtgaggtta cccctcaccc tgtcatatcc gtaaaaacgg catacagaat
    11041 atcacatcag ccactaaaga cgttacctgc gcccggagcc gttttcagca cccaaacccg
    11101 tccatagtgg ttgtaccaac ctgcgcggtg tccggcttcc ggcccgatcc cttgatagat
    11161 atcgaagtgt tggcctttga ttgcgccacc gacatccagc gccaccatca gacgcaattc
    11221 gtactgaccg ttaaatttgc cgttattatc cagcagcggc acttctgcca gcaaggttgt
    11281 acctggcgga ataatggaac gatcagaggc aactgaagcg cgaccaacca gcggcaccgc
    11341 acttgcccct ttcaccggtg caaaagattg cggtttaaag aagacaaaag acgggttctg
    11401 ttccagcagc tcgcgaacct cggcttcact gtgtgtttcg ccccagtgac gaatcgcctg
    11461 catcgacata tcttcttttt tcacttcgcc acggtcgatc agcaccttac caatgctgcg
    11521 ataggcatga ccgtttttcc ctgcatagct gaaaaagtta agcggactgc catcaccaaa
    11581 gtcgatatac ccactaccct gcacatccat aatgaagtta tccatcaggg agttactgta
    11641 agcgagaata tatttatcac tcaatgcccc cgcgtagatc tccgcacgag acggcagacg
    11701 accacgtttt ggcggcatac ggtaaatagg atactggaac tcgccctggc gggtatggcg
    11761 cgcctgaatt accggcgtgt aatagcctgt aaactgcacg tttccatagt tgtcggcacc
    11821 ttccatctgc caggcatcaa tgccgaactg gcgcatattg cgggtatcac cgcctgcgcg
    11881 cagccactct tgcaccgcgt tataaacatt actctggttg ccatacagac gcggtgacga
    11941 attacggata tggttaattt gctcggcaaa atcaccggcg ttaatcggcg cgccaacggc
    12001 atctggctgg ttcaccagag agaaaggctg ggtaaatttc ccgtctttat attgctgtcc
    12061 gcgatcggtt ggtttggaag agcaggcggc aagcattgcc acaaccgtgc ccataagaag
    12121 gtactttacc caacgtcctt tcattgttct cttcttcagg ttaaaaaata aggcgcaacg
    12181 aagataacaa accgccgcgt ttaatgaaat gagcagcctc tccctgacgc aaattttgca
    12241 caaaaaatag gctttagtga tttgtttttg ttcaaaatca tgccaaatcc gtgatcgggg
    12301 taaaaaaaag gttgcatgaa aacgcgagcg gagtatagtg cgcatccacg gacgcggggt
    12361 ggagcagcct ggtagctcgt cgggctcata acccgaaggt cgtcggttca aatccggccc
    12421 ccgcaaccaa ttattgaaca ccctaacggg tgtttttttg tttctggtct cccataaaaa
    12481 agcgccattc agcgcctttt tatcatcccc ttctcgccag cgtcgcccca tcggcaaaat
    12541 acgctttaat ccccgcaaga atagactccg caacttcctg ctggaaagtc gccgttttca
    12601 gtttacgctc ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat
    12661 ctggtgcctt tagtacggca aacccggcct gttcaacttg atttttatgc agcttgttga
    12721 ttttaccgag cttattcagc actgctttac caaacttcag gctgtcggca atggtcagcg
    12781 actgcaccat atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac
    12841 caatcaagtc cgaggcgttc tgggtttgtg ccagatattt tgccgcagta ctggttgcac
    12901 ctttggttga gagcgcaaac acagaggaac cgctcggctg acgactggta aaggcgtcgg
    12961 catggataga gacaaacagg tcagcacgct gtttctgagc ttttgctacg cgcacttgca
    13021 acggaatgaa gatgtcttca ttgcgcgtca tgtacacctt catattgccc tctttctcga
    13081 tcagagagcg cagacggcga gctatttgca ataccacgtc tttttcgcgc gttttgtatt
    13141 tccccaccgc accggagtct tcgccaccgt ggccagggtc aagcataatg acaatcggac
    13201 gatcgcgccc tgctttacct ggttgtggac cactttgcgc tggtggcacc tgcttttcga
    13261 ggtcgccttt gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat
    13321 tggccggata gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa
    13381 acagctgcgg ttttacgttt tgctttaatt caaaaaccat ccgtacggtt tgcgggtcaa
    13441 attgcccgac gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc
    13501 ccttgagtac cgagttcagg tttacatctt cgatatccac caccacgcgt tccgggttac
    13561 tcaacgcgaa ctgcttatat ttcagctgac gatttgattc taccgtcacg cgggtgtagc
    13621 tggacgcagg ccagacgcgc accgccacga cctggctgac cgcagccaga ctgacctgac
    13681 ttacgctcaa tagccacatg gcacccgcgc cttgcagtaa acgacgacgg ctgattgcag
    13741 tgttggatcc tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac
    13801 gactaatttg accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa
    13861 acattctttt tatattcacg gcattactga taaaaaagtc gctctcgcat aaaatttaca
    13921 cttgcaccct gcgaaaaaac agaataaaaa tacaataatt tcgaataatc atgcaaagag
    13981 gtgtgccgtg gtaaaggaac gtaaaaccga gctggtcgag ggattccgcc attcggttcc
    14041 ctatatcaat acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga
    14101 gcatgagaat ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg
    14161 tctggtggtg gtctatggcg cacgtccgca gatcgacgca aatctggctg cgcatcacca
    14221 cgaaccgctg tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa
    14281 gcaggctgcg ggaacattgc aactggatat tactgctcgc ctgtcgatga gtctcaataa
    14341 cacgccgcta cagggcgcgc atatcaacgt cgtcagtggc aattttatta ttgcccagcc
    14401 gctgggcgtc gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga
    14461 agacgcgatc catcgtcaac tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt
    14521 ttcagtcact ggcgagagct ttaacctgac ctcggaagag attgccactc aactggccat
    14581 caaactgaaa gctgagaaga tgattggttt ttgctcttca cagggcgtca ctaatgacga
    14641 cggtgatatt gtctcagaac ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga
    14701 agagaaaggc gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg
    14761 ccgcagcggc gtgcgtcgct gtcatttaat cagttatcag gaagatggcg cgctgttgca
    14821 agagttgttc tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat
    14881 tcgtcgcgca acaatcaacg atatcggcgg tattctggag ttgattcgcc cgctggagca
    14941 gcaaggtatt ctggtacgcc gttctcgcga gcagctggag atggaaatcg ataaattcac
    15001 cattattcag cgcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga
    15061 gaagattggg gaaatggcct gtgtggcagt tcacccggat taccgcagct catcacgggg
    15121 tgaagttctg ctggaacgca ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt
    15181 tgtgctgacc acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cggtggatat
    15241 tgatttattg cccgagagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt
    15301 gatggcggat ttagggtaag cgatgaaaat cgccggatgc ggcaggcgta acactcgtgc
    15361 gtcgcatccg gcaattacgt ttattcccgc gaactaaaca acgccgccag accactgcgc
    15421 cgctcagtac gagtggcgat tgccgcactt aatatacgct catcggcata cagcgacaga
    15481 cggcgacgcg cgcgggtcac cgcggtgtat accagctctc gcgttactac cggcgtgcgt
    15541 tggctcggta aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc
    15601 atcgcccacg tagtttcgtg ctctggcaag cgactcggtt gcacagactt aatattgccg
    15661 tccggcatcg caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg
    15721 ccattaaaca acccaagcgc gctgtcatta cgggcaatca tcaccggtcg gccttcgtac
    15781 caacgagagt gcggattacg atgaattttg cgcttctgtt gcataaactg ctcaattcgc
    15841 tcattcagtc cagccacgcc aaatggccct tcccgcaggg cgcacaaaag ctggtactca
    15901 ttgaacgcct gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt
    15961 ccgtaacccg caagagcttc ctcaagcatc gcaatataat cttcgccgct ctgtaaaagt
    16021 cgtttttcga tatcagtaaa atcctgctga aaaacggttt tcactgccgt tttatcacca
    16081 cggttgatcg ccgcagctaa ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt
    16141 tgcagcaggc agagactgtc gcgcaaagat gccgcttctg tgccagttcc tgccggaacg
    16201 tgagtccccg tcaggcggct tagctgcctg gcacgctcgg cggtaaagcc cgcgttggca
    16261 taagcgcaga tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg
    16321 ccgagaaaga tcactcgcgc atgatctggc aaggcgtcga tcagtctcga catcataggc
    16381 agatcgatca ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca
    16441 tgatgacgta aacgctggct acccggctgc gcgcccagca atcggtgcaa agtgctggca
    16501 tcttccggaa tgcgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg
    16561 agagattcgg ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag
    16621 cgttcgccgt ctgccatttg aattaacgct gccagcaact tcgctacggt ggtcgtttta
    16681 ccggtgccag ggccgccgga aatcaccgag atccgccgcg tcagcgccac tgccgccgca
    16741 actttttgcc agttaatttc atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt
    16801 aaagcttcat caacctcaat ggcatgattc acttcgttga aaaagcgtgc cactgtgcgc
    16861 tcgttacacc acatgcgatt caaataaaga cgatcaccac agaggatcat cggcgtgggt
    16921 tcatctcccc ggctgaccgc ttgtgaagcc agcaagcatt cttcccaatt ttgtagctca
    16981 ccgatttcac tgacacaggt cgccaacagc ggatgcgacg cctcattatt ttccagtcgt
    17041 gaaagcggca aacaaacgtg tccctctccg gcatcatgac ttaacagtgc cgccgcgagg
    17101 gtgacggcag gatgttcatc tcccgccacg gtcagggcaa attgcacatc cagcgggcgt
    17161 agctgtttgt gctccacagc ttccagtaat tgcttctgca atttcattac gcctcctcca
    17221 gggtcatacc ggcaaacatc tcatccatca gggtaatcaa ctcggcgttg gggcgagttg
    17281 tgtaaatccc ctgttgcgga tgttctttat caacgccacg caggaacaga taaataacgc
    17341 cgccaaagtg gcgctcatag tcgtaatcag caatgcgatg gcgcagataa cgatgcagcg
    17401 ccagggtata aagctgatat tgcagatcat agcggtgtgc ctgcattgcc gctgccatag
    17461 cctgttgggt gtaagccgaa ctgtcttcac ccaaccagtt ggatttatag tcgagcaggt
    17521 aataacgccc ttcgtggcgg aacaccaggt cgataaagcc ttttaacatg ccacgtacct
    17581 gcatgaactc cagcggcggg cagcctgcgg atagcgggtc aaactggcgg attaacgtat
    17641 caagctgact ggcgataagc ggttcactaa tcggcagata aaactccatc tccacctgtt
    17701 tattgcgggc ggaaagttga ctcaggctta cgccggtttc attgagaggt gcctggagga
    17761 cagccgtgat ccactcggtc aataccggtt cccactgcga ttcaaagccg ccgagttcca
    17821 gtttttcccg cacccagttc gggtcaaccg gctgggtaaa atccaggtct tcaaacaaac
    17881 tgtgcaagaa cgtccccggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt
    17941 cttcaacgac gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag
    18001 cgataccgtg accacgctgc tgcaagccgg agtagctggt gacacgccag ttatcgccgg
    18061 gcaatcgttg taacgtcttc gcattcagct ctgctgtaga aacatcatta acctgccagg
    18121 gttggttatc accagtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa
    18181 tacaggtgcg aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg
    18241 cactttggtg gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa
    18301 cgccgagact acaatgccaa accgaacgcg tcagcgccac ataaagcaaa cgcagatctt
    18361 ccgccagacg ttcggcctcc gcgagatcga cactttctgg cgcagcgtta agatccagaa
    18421 cagcctcaaa cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa
    18481 taaatggcaa ccagacaagc gaatattcca gcccttttga tttatggatc gtgacaatct
    18541 gcaccagatg tttatcactt tcgaggcgca tttgttggct ggaggcatta ctgtctggct
    18601 cgaggatatg ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg
    18661 cttcttgtag cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg
    18721 ttgccagcaa gttttctgca atgttacgcg ccgacatcag cgcccgcagc atcggcataa
    18781 cgccacgttt gcgccagatt tgccgataac catcgaactc ttcgaccacc gcgtcccacg
    18841 catgttcgtc attattcagc gtttcgatat ccagcgcgtt cagccccatc attgacgttg
    18901 ccagcgcact acgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacaaccaaa
    18961 gcatttcctg cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag
    19021 ggatttccag caacgttaag gcatcgcgca cctgggcagc ctcctggcgg ctgcgcacca
    19081 gcacactgat gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt
    19141 cgccccgctg tccggcttgt agccagtcgc ggatttgcgc agcacatacc tgcgccatgg
    19201 tactttgata gtcgccaacg ccgcagcttt cgccttccat cagccacatt ttcatcgcag
    19261 gctgcgtttc acctttaaat acaaaacgta acgcctgatt tttcccggct gatttcactg
    19321 gaataaacgg tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa agcttgttca
    19381 cgctgttcac cattcctggt gcggaacgcc agttggtgtc taaagtgtag tgggcgtgaa
    19441 cttcgctacg cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct
    19501 gcttcgggtc gccaattagc aacaatgcgg tttccggctg atggtgccag atacggcgaa
    19561 aaattcggta ctgctggggg tcggtatcct gaaattcatc gatcattgcc accgggaatc
    19621 gcgtacggat cgccgctgcc aacacctcac cgctttcgct acgcagcgcg gaatcgagcc
    19681 gacttaacat gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt
    19741 cgcggatctc agccaatgcg cgggtgatca ccagatcgcg gatcgacaat ggttctgcaa
    19801 gcagttgatc gatcgcctca aacagtggat gtcgcggggt ttcccccccg gccttcgtgc
    19861 gatcttctaa gaaacgctgg gagaattttt ccagcgactc cggcaactga taactgtttg
    19921 tctcttcttc tgcccaggcg ctgatcttgt cgatccattt agcctgattg ctacggttaa
    19981 acttgcgtcg atcaatacca gaagattcga tcagcgcatc cagttcaccc actgcgtcgc
    20041 gccactgctg ttttaccgta tcaatacgcg ccacaatttg cgcgtgacgg gaagccagcg
    20101 tttcatcatc gggcggcggt gctttgataa ccggcgcttc gccttgcaga taacgattaa
    20161 tatcgctcag caacgcctgc ggccctttcc aggtttcaaa gacgacctgg gctatttcac
    20221 gcggcagcgg gtagcagtgg cgacgccaga aatcggcgca ggcctggtag cgtagccgag
    20281 actcatcttc aatcagctgc tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga
    20341 gcatgcgctg gcaaaagccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg
    20401 ctaacaacaa ccactgcgcg gcttgcgctt tatcgtcgat ctcttccagc agacgttcgt
    20461 acagtggatt gtcggtggtt tcacgcagac aggcgatgcg caactcgtgg atattgctac
    20521 ggatacgacc gcgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt
    20581 caacggtcag cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat
    20641 agagcgccgc aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc
    20701 cctgtaaggg caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc
    20761 atcaggggca ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcagga
    20821 tgcacatatt cggctttccc gttctggctg ccggaaatct gcgacagaat agccatgcct
    20881 tgcggctcga ccaccgcctg atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt
    20941 atctgggcca cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta
    21001 cttaacttcg atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc
    21061 gcctgctgga tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt
    21121 gggaaaaacg ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa
    21181 aggaagccca tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc
    21241 aattgttctt cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga
    21301 gagctatagg ctgagctggt gtattcatcg tagccagtcg gtacaaatac cgctgccagt
    21361 gcggagtcgg tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact
    21421 acatctttgt ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt
    21481 gccagcgttg ttgcctgggc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc
    21541 cctgatttta aggcgtcgcg ataggccagc acctctttca acgtaatgga gggcaaaatt
    21601 ttacgccgtt catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc
    21661 gcctgctcaa acgctttacc cttttctgcg gaatccatca tctggttata ccaggacttc
    21721 gcctgctcaa gctgatcttc cgtagcggta tagctaaagt atccctcgag caatgcctgg
    21781 aacagctgcg gcagacgctg ggtgtaacca ttagcattaa ccataaggcc gttgttagcg
    21841 ttggtggaaa aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc
    21901 cctgcgagat aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc
    21961 ggattacgca aaatcaggct gacatcagct ttgggttcgc tggcaaaata acggcttggc
    22021 gcatacacca cgcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttc
    22081 tctgacttaa tcagcgagaa atcgtcagga atataggggt taagctccgg taaggagagc
    22141 gcaatactgg cagctttttg ctgccagtca gcgaaagttt gttcgctgat tttatcgacc
    22201 tgatacggcg catcgacaaa ataagccgtt ttattgtgcg gctctttcgg gctgatatac
    22261 cagatacgcg cattctgcgg cgtcatcatc gccagacgtt cttttactgc tttagcatcg
    22321 taccgatcgg caatattgac tgcatccagc gtatgctcaa ccggaacgcg aatcatggta
    22381 tctgccagcc attcgacgta atccatatca cgggtgattg acggataacg gaagtcgata
    22441 tccagcacat tcgccagttc atcgaagtat tgtttatcga tccctttttc acgtaacaaa
    22501 ttgaggtagc taaaaatagc cgccacaacc tgatcgcgat tcgccagacc tttatcggtt
    22561 aaagacgcag agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg
    22621 ctaatgccct caactaaccc ctgcttttgc agccagtcag acagtgtacc tgggctgcga
    22681 ttaccaatca gataggtaat caattcatcc gttttactgc ggaacttcgc tgagttgtta
    22741 tcgatgcgaa actcaacgcg cagaacttta cgcggcagcg ctgggacgta atgaatgata
    22801 atgccctttt gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg
    22861 ttcggcacgc gaccaaaggt gtccgccgcc atttttgcca actccggcag cggtttatta
    22921 ctgtaaataa ccgccttcat caaattggcg gaatagtact tctcgtggaa atctttcagc
    22981 gcctgctgca ccggattacc aggtttgtcg cttaaagttt cgaggttacc accagaaaac
    23041 tttgaaccgg ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg
    23101 tcacgcgtac gcgccatggt taattcagcg ttcactgcat tacgctcacg ttcggcgtat
    23161 ttcttgtcga gcaagggttc tgcaatagca tcggccaggc ggtctaccgc accgggcaag
    23221 gcgtcgttct caacttccag atagaaagcc gtgcgatacg gtgccgtgct ggcattgtga
    23281 ctaccgccgt gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac
    23341 cccatcagac tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc
    23401 agcgacccaa cgggcaccac cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc
    23461 agcaagacca ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca
    23521 cttttacgga tggtttcctg aatcggctgc catcccgttt ctgcctgact taatggtgcc
    23581 caaagggcaa ctaacaacaa taatgctttg aaccaggtgc tgcggggcat tcacggacct
    23641 cataatcaac tttattttct ggccagattc aacaggttaa cgcaaatcat ctgccagaat
    23701 ttaatcttgt gctgcacgag tcagcctatg tttatataac catcagtccg tgactggtgc
    23761 gcatcataaa gtaagcggat agattgcgca atttttatac agcgctcatg actgattaaa
    23821 gcgaaacagc ggtaacagga aacgttgcga ctgttcaacg attgcctcca ttgtctctgg
    23881 tgttaattgc cgccagagcc tttgatacca gatatcatca ccttcgccac gcaccatcat
    23941 gttgccttcg taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc
    24001 cagcatggca tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg
    24061 taacaccagc aatggcgcgg acatcccttc acgatacccc tcaatcagtt gtgagaggta
    24121 atgcaaagcc tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa
    24181 aaggcgactt tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg
    24241 cattccctgc gccacactta ataaagaggg acgccagcgc aacaagccat ccggctgcac
    24301 ctgcggcaac caaccagtta tctgcacacc gttgcaagcg agatcaattt ccatactctg
    24361 ccccggctgg cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgtgt
    24421 ttcccagaaa atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcggcg
    24481 gaacaagcgt tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg
    24541 ataacggcta agtccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcagt
    24601 acggaagttc acctgcaaac gcatctggaa gaacgcccgc accggatgtg cccagaatcg
    24661 ttgtagcgtt tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc
    24721 agaatgtgct ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg
    24781 ttcgcctggc tgatagtttt gtggatcaaa cggcattcgg gtatggaggc aagtaagatg
    24841 cgcttttacc cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg gtagataatg
    24901 actttgcccg atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt
    24961 atcctgaatg gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc
    25021 ttccaggaac agatagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg
    25081 gctcatcagg tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc
    25141 cagcaggcaa accactttga acggaattga acgcattggc atcagagtac aaatgttaac
    25201 cggcccggcg agaaaacgct ggctgatacg ttcttgatcc aggcgctgtg ccagttcatc
    25261 acgcaatagt gacagcggca ccgcatcgcc atactgcgca cctaaacctt cggcgataat
    25321 cgcctgccat tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa
    25381 gaaggcgttg agcatatcgc gacaaaccgg caaccactct tccagcggac gctcctgtgc
    25441 cagcccgcga cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc
    25501 tgcaattaag ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct
    25561 ctccatcgcg tagcccaaca acatacgcgt caggccaaat cgccaggtgt gttgtccggt
    25621 ggcggggagt tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc
    25681 gttgacccac tgacgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag
    25741 caccggcaca tccagtaatg ccagcacgtc ttccgacaca aagcggctgt ctggcaacga
    25801 taacaggctg ataaacgctt caagtacagg atgcgactgc cgcgcccgac ggtcggaaat
    25861 ggcgtaaggc aggtaacgat ccgcaggtgc gctaccaaac acagcctgaa taaacggact
    25921 gtagctgtcg atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc
    25981 ttccagcatc gccagcaggc gatcgtgcaa aacttcaact tcacgctgcg ggctatggca
    26041 aacgtggaag gtgatactgc tatccagtgg atcaagcggg cgtttgttat cgctacggga
    26101 aaactcttcg atgttcacac cagcaacggc gcggttttcc agttccagaa tgtcagactg
    26161 aatgttatgc agcaggttat ctggcgtgac atcgacaaag gcgtccagct cctggctgct
    26221 ctccaggtca gaaaggagat aaatgtagtc gcgcccaagc ttaccccatg aagccagcag
    26281 cgggttgccg acatcctgtt caccatcgct gttaaagagc tgcccggcat tttcgctatc
    26341 gcgaaatagc ggcaattcgc gatcttcaaa actgtgtcga cgctgacggg tcagcagttt
    26401 cgccagataa gcaggatctt taatatcgcc ccagtaataa cggcaggggt tggtaaacag
    26461 gagatggatt tcaatatgtt tacccagcgc ctggagcgcc tggagataaa caggcggtaa
    26521 cgcggaaata ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga
    26581 ctccagcgtt tcgataaagc gctgatagag attcgcgcgg tgccagcgcg gttgcccgag
    26641 ttcatgggta tattccacca gagccttcca caacggcgct tgccaggcct gtgcttctcc
    26701 cagcccttca accaaatgtc ctgtttccca ttgtgccagc cactccgggc gatagaccag
    26761 atactggtca aacaggtccg ccgcttttga ggaaagctgg aacagttttc gcttgtcgct
    26821 atcgtcagtc agataatgcc gcaacagggt aaagtcttcg cgctccagca attgcggcag
    26881 cagagtcatc agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatctc
    26941 cggtaacacc cggacgaaca tatcccagat aaagctcgca ggcagcggaa aatcaatgtt
    27001 tgccgcaata ccaaactttt gcgacagggt catttgcagc cactgtgcca taccggtact
    27061 ttgcaccaga atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa
    27121 ctccatcaac gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc
    27181 tcctgactac tgacgattcg ggcaatgcag gcgggtcatc tcgcctgctc tgcccccggg
    27241 tgaaactagc gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac
    27301 ctgccagttg gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca
    27361 gagctgttgg tactggtttc tgctggcaag actgttcatt aatgttcgct ggaaacccga
    27421 taacgcagtg acaatcatca ccatcaacac catcgccaac attacctccg gcaggctaaa
    27481 gccttgttga ttcttcaggg aagctgacat aacgccccct ctttcagcgg acaaaaatcg
    27541 ctccagccgc gtggcgaaaa aacaattttt ccatcgatga cttcacctgc tcgccacaac
    27601 gaaacgcctt cataaccggc aatcaataag gcttcattat ctgccaataa acgcaaacaa
    27661 acccgggcat cggttccagc gtactgcaag cactgaactg ctggctgcgt ctgccaggag
    27721 tacatttttc cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt
    27781 tccatgctca cgcgagaggc aaaactgcga tcctgttggc tcattccttg taatagcaag
    27841 ctacccaaaa ctagcagcat caggaccaga gccagtgacg aaacaccctt ttcgcggttc
    27901 acaggttgaa tcctgtcacg ctatagctgg cattcaccac agtttgcggt tcagacttac
    27961 tggcagcacg catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga
    28021 cctgaaaagt gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt
    28081 cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct
    28141 ggtcggactc ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca
    28201 caatgacaca gtcaccctgt ccgacaattt ccagcccttc gccggtacag atgccatgac
    28261 aataacccgc cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt
    28321 ccagtgccag cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg
    28381 cagcccccaa caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa
    28441 aaccttgctc ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc
    28501 ccacggcgaa accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc
    28561 ccatgcggta ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac
    28621 ttcgggccag cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tcgccccagc
    28681 aacggaactc acaaggcacc ataacgtccc ctccctgata aaactgatac tgtggtcgcg
    28741 gttatgccag ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt
    28801 ttgccaaagc cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag
    28861 cgtgaccaaa atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt
    28921 catgccggca gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc
    28981 gcatcttccg gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg
    29041 aagaatttaa tggagggtaa aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt
    29101 ttcgtaatta gatagccacc ggcgctttaa tacccggatg cggatcgtag ccttcaatct
    29161 caaagtcttc gaaacggtag tcgaagatgg attcgggttt acgtttgata atcaacttcg
    29221 gcagcggacg cggttcgcgg cttaattgca gatgagtttg atccatatga ttgctgtaca
    29281 ggtgcgtatc gccaccggtc cagacaaaat cacccacttc cagatcgcac tgctgcgcca
    29341 tcatatgcac cagtaacgcg tagctggcaa tgttgaacgg caggccgagg aagacgtcac
    29401 aggagcgctg ataaagctgg caagagagtt tgccgtctgc cacatagaac tggaagaatg
    29461 catggcacgg tgccagcgcc attttatcca gttcgcctac gttccacgct gaaacaataa
    29521 tacggcggga atccgggtcg tttttcagct ggttcagtac cgtagtgatc tggtcaatat
    29581 gacgaccatc cggcgttggc caggcgcgcc actgtttacc atacactggc ccgaggtcgc
    29641 cgttttcatc ggcccattcg tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt
    29701 tagtgtcgcc ctgcagaaac cacagcagtt catggatgat ggaacgcagg tggcaacgtt
    29761 tagttgtcac cagcgggaat ccatcctgca ggttaaaacg catctgatga ccaaaaatgg
    29821 aaagcgttcc ggttccggta cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca
    29881 tcagttctaa atactgtttc atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat
    29941 atgcccagac catcatgatc acacccgcga caatcatcgg gatggaaaga atttgcccca
    30001 tgctgatgta ctgcacccag gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa
    30061 caatgatgcg aaacgcgccg tagccaatca ggaacaagcc tgagacagct cccattggcc
    30121 gcggtttacg aatatacagg ttgaggataa taaacagcac cacaccttcc agcagcagtt
    30181 cgtaaagctg tgatgggtgg cgcggcagca caccgtaagt gtcgaaaatg gattgccact
    30241 gcgggttggt ttgcagcagc aaaatatctt ctgtacggga gcctgggaac agcatggcaa
    30301 acgggaagtt cgggtcaacg cggccccaca attcaccgtt aataaagttg cccagacgcc
    30361 cggcgccaag accaaacgga atgagcggtg ctataaaatc agatacctgg aagaaggaac
    30421 gtttggtgcg gcgggcgaag ataatcatca ccacaattac gccaatcagg ccgccatgga
    30481 aggacatgcc gccgtcccag acgcgaaaca gatacagcgg atcagccata aactgcggga
    30541 agttgtagaa cagaacatac ccaatacgtc cgccaaggaa aacgccaagg aagcccgcat
    30601 agagtaagtt ttcaacttca tttttggtcc agccgctgcc cggacgattc gcccgacgtg
    30661 ttgccagcca cattgcaaaa atgaaaccca ccagatacat caggccgtac cagtgaagcg
    30721 ccacgggtcc tattgagaaa atgaccggat caaactccgg aaaatgcaga tagctactgg
    30781 tcatctgtca ccacaagttc ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg
    30841 ccgcaggttt caggcgctcc aaaggtgcga ataatagcac aaggggacct ggctggttgc
    30901 cggataccgt taaaagatat gtatatgatc cgcgctataa ccctccgcga atcagcccgc
    30961 ccatgccgcg acgctccata aaggctgcaa cctgatggcg aacttcggtc gccagttgcg
    31021 cttccagact acgctgcgca agattttctg cttcggcata atcaatgcgc cgcagcaggt
    31081 attttacccg cgctacagaa cgtccgttca tagacagatg gcgataccca agcccaatga
    31141 ggattgccac gcacatggga tcgcccgcca tttcaccgca caaacggaga tcgattccat
    31201 gtatttccgc ttcccgggcg atcatcgcca gagctcgtaa cattgcagga tgaagactgt
    31261 cataaatgtt cgccacccgg gtattgttgc gatcaacggc cagaatgtat tgagtcagat
    31321 cgttggtgcc aacagagatg aaatcgaccc gctttgccag atgcggcagc ataaatacca
    31381 ttgacggtac ttccagcatg atgccgatac gtggtttggg aatttcgtaa ccgatcatct
    31441 cctcgacttc acgtccggca cgttcaatca ggcggcgagc ttcgtcaact tcatcgaggc
    31501 tggtgaccat cggcaacaga atattcaggt tgcccgtagc ggcgttagca cgcagcatcg
    31561 cccgcacctg gatcaagaag atctccggct gatcgagcgt aatgcgaatc ccacgccaac
    31621 ccaggcatgg attctcttcg ctgatcggca tgtaaggcag ctgcttatct gctccgacat
    31681 ccagcgtacg caaggtgacg ggtttatcat tgaacatttg cagcatcccc tgatactgcg
    31741 ccacctgttc ttcttccgac gggaaaccac tttgcagcat gaatgggatt tcagtgcgat
    31801 aaagaccgat gccatcaata cggctgccca gtttttcttc atgttccggg cttaaaccag
    31861 cattgagcat gacttttata cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat
    31921 cttccgccag acggctaagc tcaatctctt cactaattag ccgctgatat tcttgcagca
    31981 gtaccggctc cggatcgacc agcaattcac cgcgatagcc atcaacgatc agcgtcctac
    32041 gatgcagcac cgaaggctga atatccgcgc ccatcacggt agggatcccc agcgcacgta
    32101 ccatgatcgc agcatgggag ttggctgcgc catctcgcac gacaacaccg actaagcgat
    32161 cctggggcag ctcagcaaga gttgtcgctg acagttcatc tgccaccaga atgaaacgtt
    32221 ccggccaggc gttcggccct tgattagcgt catcaagatg aaacagcaat cgctgaccca
    32281 gcgcacgtaa atcgccagcg cgctctttga gatagttatc gcttagcgcg gcaaactgtt
    32341 cggcaaattt ttcaatgacc gtttttaccg cccactctgc caccgagcct ttatcaacct
    32401 cggcaaacaa ttcgcgacgc agccgggtgt ccgaaagcag gtgcgagtaa agatcgaaaa
    32461 tagccgccgt ttctttttgt gcaccggcgg caaagcgttt gctgtagcgg cgaaactcgt
    32521 ttgccgcttc ttccagcgcc ccggtcagtc gttcgcgttc cagagccgga tccagcgttg
    32581 atgcctgata cacctgttcc attaaaggta acgtggcatc ctgccagcct tcggcaatcg
    32641 ccacaccagg tgctgccggt aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg
    32701 cagtcaactg cgactgagaa agaatagctg ccatctgggt ggcaagcgtc accaggaagg
    32761 attcttcact ttcgtcatac tggcgcaact ctcgttgctg taccaccagt acaccaagca
    32821 actggcgacg ttgaataatt ggtacgccta aaaacgcgcg gaagcgttct tcttttacgg
    32881 aggggatgta tttgaagctg gggtgctttt gcgcatctgc aaggtttatc ggttccgcca
    32941 gcctgccaac caggccgacg atcccttcat caaacgcgag cgttacagtg cgaccgcgtg
    33001 gttttttcag cccccgggtc gccatcaggt agtaacaacg tcgatcatga tcggccaggt
    33061 agaccgaaca gacctcggta tccatcgcaa gacagatgtc ggtaaccaga atatttaacg
    33121 cctcattcag gcgtggtgcg ctggctacct tttcgactat ttcgcgcagg cgagtgagca
    33181 taattggcgt gacttaacct cttttacgtc gataagcaga tgcgttttgt ggttttggcg
    33241 tattttcctg cagtgacatc accacactcg cgaactcttt cattacccta cggtagacat
    33301 cacgtttaaa tgacaccacc tgtctgaccg gataccagta acttacccat cgccagccgt
    33361 caaactctgg tgtactgctg gtttgcatat tgatttctgc atcgccgctc accagctgca
    33421 agagaaacca tttttgtttt tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac
    33481 gtttcggtaa tttgtagcgc aaccagttac gcgttgaagc aaggattcga acgtctttgc
    33541 ggcttaatcc tacttcttca aacaattcac ggtacatcgc ctgctctgcg gattctccgg
    33601 ggttaattcc gccttgcgga aattgccagg agtgctgacc aaatcgccgg gcccacatta
    33661 cctgcccctg gcgattacaa atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa
    33721 tcaccggact acctcaatat aaagctttat atacgaatga ttgtttcata ctccaggaag
    33781 acggtaaacc actctctgca gggcattaca cactaataac aattgaataa ctcacagtta
    33841 tgtgcagagt tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg
    33901 atagagttgt gaagaactac ggaattacta cgggaaaacc cggagcattc tgaataagcc
    33961 cgatatagac attttaaaat attcattatc agtcaatgca ttacatgtaa tttaagtaat
    34021 gaaaaagagt aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa
    34081 ccaatgccgt atttatccac agaatgtgcc actaagttaa gcactgaacc accaaaaact
    34141 ggagtttcgt cgcacgtcaa ggctgtaaat ggaaacagta gtggaggttt ttcacagtta
    34201 tcccagcttt ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg
    34261 gaaaacccgt tgcagtgttg cgcaactcga ttaccggcaa cctaaaaagc aatataaatc
    34321 agagaattga acaacgcatg tggaaaaagt tacactgcga atattcggca cataattgct
    34381 gtttgttttt taaccaaggt atcatgacat gtcccaacct cgcccactgc tctctcctcc
    34441 cgaaactgaa gaacagttgt tagcgcaagc acagcaactt tctggttata cattgggaga
    34501 actggcggca cttgccgggc tggttacgcc agagaattta aaacgcgata aaggctggat
    34561 tggcgtgtta ctggagatct ggctaggtgc cagcgcagga agtaaacctg agcaagattt
    34621 tgctgctctg ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga
    34681 aacaacattc gtttgtgttg ccccgttaac gggcaatagc ggggtgacct gggaaaccag
    34741 ccacgtgcgc cacaagctca aacgcgtgct gtggataccg gttgaaggcg agcgcagcat
    34801 cccgctggcg cagcgtcgcg ttggatcacc gttgctgtgg agcccgaatg aagaggaaga
    34861 ccggcagcta cgcgaagact gggaagaatt aatggatatg attgttctcg gtcaggttga
    34921 gcggattacc gctcgtcacg gggaatattt acagatacga ccgaaagcag cgaatgcgaa
    34981 agcgcttacc gaagccattg gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt
    35041 ttatttgaag aaaaatttca ccagtgcgct gctggcccgt cattttctga tccagtagcc
    35101 atcgctttga cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct
    35161 ttttagatgt tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacgctg
    35221 acgctgctgg agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca
    35281 aagcttccca cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtt
    35341 atgcgtctgg cgctgctggc atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt
    35401 acaatattca gtcaggaaat ttctgcccgt gacttgattc tgcttctggg tggcttgttc
    35461 cttatctgga aagccagcaa ggaaatccac gaatccattg aaggtgaaga agaagggctg
    35521 aaaacacgcg tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc
    35581 ttcagcctcg attcagtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg
    35641 gcagccgtgg tgattgccgt aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt
    35701 gtcgaacgcc atccttcggt aaaaatgctg gctctctctt tcctgattct ggtgggcttt
    35761 accctgattc tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg
    35821 ttcttctcta ttgcggttga aagcctcaac ctgattcgca acaaaaagaa tccgctctga
    35881 tactccgtac gctctcctgc gggagggcgt actcactcct gttaccctca cagtttaaga
    35941 ttttcctgct ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtgggc
    36001 caaatgagga taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt
    36061 ttgctgtttc cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt
    36121 tgaccgatgg aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc
    36181 aaggtaacgc gatgcaaatt aaccgtgatg atgtttcgca aataattgaa cgttaacaaa
    36241 taaggtcagc atccggctgg ccttaagatt tttctctttc ccttttccct tccctctgcc
    36301 atttttatat tccttatgtc gtgattataa aaaggaaacg gctatgcaat atcaccgtat
    36361 accccacagt tcgctggaag tcagcacgct ggggcttggc acgatgacgt ttggtgaaca
    36421 gaacagcgaa gccgacgccc acgcacaact cgactatgcc gtcgctcagg gcattaacct
    36481 tatcgacgtt gccgaaatgt acccagtacc tccgcgcccc gaaacgcaag ggttaaccga
    36541 aacctacgtc ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc
    36601 caaagtgagc ggaccgtcgc gcaataatga caagggcatc cgcccggatc aggcgctgga
    36661 tcggaagaat atccgcgaag cgctgcatga cagcctcaag cgtctgcaga ctgattacct
    36721 cgatctttat caggtgcact ggccgcagcg cccaaccaac tgcttcggca aactcggtta
    36781 tagctggaca gattctgcgc ctgcggtttc gctgctggat acgctggacg cactggcaga
    36841 gtaccaacga gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg catttggcgt
    36901 aatgcgctac ctgcatctgg cggacaaaca cgatctgccg cgtattgtca ccattcagaa
    36961 cccttacagt ctgttaaacc gcagttttga agtaggtctg gcagaagtca gccagtatga
    37021 aggggtcgaa ctgctggcct attcgtgcct gggtttcggc acgctgaccg ggaaatatct
    37081 caatggtgca aaacccgctg gcgcacgtaa tacgctcttt agtcggttca cccgctatag
    37141 cggtgagcaa acgcaaaaag ccgtcgcggc gtatgttgat atcgccagac gtcatggcct
    37201 ggatcctgct cagatggcgc tcgcttttgt acgtcgtcaa ccgtttgttg ccagcactct
    37261 gctgggcgca accacgatgg agcagctgaa aactaacgtc gaaagtttgc atctggagtt
    37321 gagcgaagac gtattagctg aaattgaagc ggtgcatcag gtttatacgt atcctgcacc
    37381 ataagattaa cgccggttaa aaccggcgtt aaatattaat gacggcgctg ccagatccac
    37441 agcgccgtta ttgccagcgc aaatagcgca ccgaagccaa tgccaatggg cacgaccggg
    37501 atgcctacca ttaccgccag cgagtaaatg cccagcatca acaacatggc gctattttcg
    37561 ccaaggtttt gtactgcaat cgcattcccc gccccgacgc tttttttacc ccgctcctgt
    37621 agcaacgcat tgagcggaac gacaaaaaag ccccccagca cgccaatcag catcaacaag
    37681 gcataggctg gcagcagctc gtgttgcagg gagaaaatca gtaccaccac gccaatcaaa
    37741 atcccggctg gcatacagcg tgacacggtt tccagcgtaa ccagcttcgc tgccgcacct
    37801 gcgccaacca cgataccaat cgctaccatc gcgttgagat aggtcggcgt agcgttatcg
    37861 gtaatgccca gcgccaccgg tacccacagc accaacagga aacgcagcgt gacacccgct
    37921 ccccagaata aactggtgcc caccagcgaa aaacgcgttt caccattgca ccatagcgag
    37981 gtgcaggcat tcaggaaact gcgggtcatg ttgatgagat tccaggactg ccccggacgc
    38041 gccgccgcca gtttgggaat gtagatattg gcaacgaccg caccaccgta ggccagtgcg
    38101 catgcggcca gggcgaccag gacatgccag tcagccagca caccaccggc tacggaaccg
    38161 agcaaaatcg ccgctatggt agaagcttcc attaaaccgt tagcttttac taacttacta
    38221 cccgtggtta attcgccgag aataccgtat ttcgccggtg aataggctgc agcaccaaca
    38281 cccaccagcg tatagccgag aaacggattg ataccaaagc agatactggc tgcgcccagc
    38341 agcttcaggc cgttggcaaa catcatcacc cggcctttgg cgaagctatc cgccacctgc
    38401 ccgacaaacg gcgcaaaaag aatgtaagca cctacaaaca ccatttgcag gatgggctgg
    38461 ctccactccg gatagaactg cgctttcagt aacgccagag tggcaaacag tagtgcatta
    38521 tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt tcatcccctt cgaccacaac
    38581 gaagtattag tgtgcactga ctcactcatc gtgttgttcc gcttcgtcta cccagctttt
    38641 caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc atctgtttca gataacgaat
    38701 atcacgcggt acagcaagct ccggcacacc gtgctcgcgg gcatactgtt gcaacttatc
    38761 gcgcgtcagt tcgttatctg tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct
    38821 cttaattgca gtggcatgga ctttatctgg cgaaacacca agtgccagtt gttccaccat
    38881 ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt tttgcgcgac cctgaatctg
    38941 cacaaagccc tgctcgtcaa aacgcacgat atcgccagtg tcgtaccagc cgcgctccat
    39001 ttcgccgcgc acattctcgg cggtgggcac ttccagtaca cctggcttct ccacccgcag
    39061 atagccgttc attatgttcg gccctttcag ttgcaggcgt ccgccctctt cgataccagg
    39121 aaccgacaac aggcgcgcat ccattcctgg tagaatacgc cctaccgtac cgggtttcgc
    39181 cgccatcggt acgttgatag aaacgacagg cgcgcactcg gttacaccat agccttcaag
    39241 gatgcgcagg ccaaatttat cctgccaaag ctgtttggta ctttcttgta gtttttctgc
    39301 gcctgccacc acatagcgta gacgatagaa atcatacggg ttggcgaaac gcgcgtagtg
    39361 accgaggaaa gtggaggtac cgaacaacac ggtgcaactg cggtcataca ccaactccgg
    39421 cacaatgcgg taatgcagcg ggcttggata aaggaacact tctgcacctg tgagcagtgg
    39481 cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc ggtaacgccg acataaagcg
    39541 atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg acattcgcca gaatgctttt
    39601 atggctatgg acgacgcctt tcggatggcc ttcagaaccg gaggtaaaaa ggatcagcgc
    39661 ctcttcttcc ggctgctgtt taacctgtgc cagacgcggc atcagcaaat gagcgaagat
    39721 ccatactttg tcggcagtgg tgacatctgc ttttaaatct tccagataga cccagcgcac
    39781 ctgagtaagt tgctccggca gatgccagag tttgccttta tcgagaaact ggcgggaagt
    39841 gaagatggtt ttgatttcag ccgccgtaat ggcgctggtc agccctttta ccccagcggt
    39901 gtagttcatc attgcgggaa tgcggcgacg ggcgatggcc ccaaaaatca ctgccgcact
    39961 gatgcctgca ttgggcagca ttaagccgat gcgttcgcct tcaacactgt atttttcaag
    40021 gatgcgtcca acaaacagcg ttttagtaag caatttgcga taggagtctg gggtaaagtt
    40081 gacgtcttcg acacatttct tcccggctcc gaagcggtac attgcactca gtaaagattc
    40141 gtacagcgtt tcacgcgggc gcaccgccat acgcgcttcc atcattattt gatgcagcat
    40201 ttcgccagcg attttgcgac ggtcacgggc acgcggcgca tccggcatct ccacctgcgt
    40261 tggtggcaaa atatgcagag taatttgcgg gaacaagcga cgtttaacca gacctttcag
    40321 gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca ggaataaccg ttgcaccaga
    40381 cttcgccgcg acaaaacccg cgccatcgta gattttcatc agcgagcctg tggtggtgat
    40441 gcgtccttca gggaaaatca ccactggtcg gccctgttcc accagacgta ccagatgttt
    40501 aatcgccata ggttgcgtcg ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg
    40561 catataccac tgttgactta ttgaggtgta aacggcaaat accggacgta cgggtaaaaa
    40621 caatgccagc agtatgccat caataaaaga gacgtgatta ggcgtgatta gaacgcgctc
    40681 gcccttcaat gcctgggtgt cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt
    40741 tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg attcagtaaa agcgaatgga
    40801 gggagattac acgagataaa gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg
    40861 cgcatccgcg caggttggtg caagagacag ggtacgaaga gcgtaccgaa taatctcacc
    40921 aatcaatacc tctgggatct tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa
    40981 cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt gtcggttact gttacagatt
    41041 gatgaccggc aaaaaaaaac ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac
    41101 ccgaagttga cttcacctat caatacctct gggatcacca ctttagcaac ctgaagccaa
    41161 acgccaccag cggtcaatcg caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct
    41221 ctttttttgt cttacttatt tgcgtttggc tcacattccc acgatgaaaa cacgccaccc
    41281 cttgaaccaa cgggcgtttt ccgtaacact gaaagaatgt aagcgtttac ccactaaggt
    41341 attttcatgg cgaccataaa ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt
    41401 tcccgcgtca ttaataattc acccaaagcc agcgaagcgt cccggctggc tgtgcatagt
    41461 gcaatggagt ctcttagcta tcacccgaac gccaacgccc gtgcgctggc gcagcagacc
    41521 actgaaacgg tcggtctggt cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg
    41581 aaagcggtcg aacaggtggc ttatcacacc ggtaatttct tattgattgg caacggttac
    41641 cacaacgaac aaaaagagcg tcaggctatt gagcaactga tccgccatcg ctgtgctgcg
    41701 ttggtcgtcc atgccaaaat gatcccggat gccgatttag cctcattaat gaaacaaatg
    41761 cccggtatgg tgctgatcaa ccgtatcctg cctggctttg aaaaccgttg tattgctctt
    41821 gacgatcgtt acggtgcctg gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc
    41881 attggttatc tgtgctctaa ccactctatt tctgacgccg aagatcgtct gcaagggtat
    41941 tacgatgccc ttgctgaaag tggtattccg gccaatgatc ggctggtgac atttggcgaa
    42001 ccagacgaaa gcggcggcga acaggcaatg accgagcttt tgggacgagg aagaaatttc
    42061 actgcggtag cctgttataa cgattcaatg gcggcgggtg cgatgggcgt tctcaatgat
    42121 aatggtattg atgtaccagg tgagatttcg ttaattggct ttgatgatgt gctggtgtca
    42181 cgctatgtgc gaccgcgcct gaccaccgtg cgttacccaa tcgtgacgat ggcgacccag
    42241 gctgccgaac tggctttggc gctggcggat aatcgccctc tcccggaaat tactaatgta
    42301 tttagcccga cgctggtacg tcgccattca gtgtcaactc cgtcgctgga ggcaagtcat
    42361 catgcaacca gcgactaacc gcagttaaag caattccagc gccagtaatt cttcgatggt
    42421 ctggcgacgg cgaatcaacc gcgcctgacc attatcaaac agaacttctg gtaacagcgg
    42481 acggctgttg tagttggatg acattgatgc gccatatgcc cctgtatcat gcagtaccag
    42541 ataatcaccc gccttcactt ccggcaaggc gcgggtttcg acatttcccc cttcctgctg
    42601 ggtaaagaca tcgcccgatt cacataacgg cccggcgacg acggtttcca ccgttggcgc
    42661 gtgttccaga gaacgaccat cagctgccag agcactgata tggtggtaac taccgtacat
    42721 tgctgggcgc atcagatcgt tgaacccggc atcaacaagc acaaagtggc ggctacccat
    42781 ttgtttgacg ctccgcacct gagtaattaa tacgccagac tgcgctacca ggaagcgacc
    42841 cggttcaatt tccagtttca cagggtggcc caaatggcgg gcaatttgct cacgcgcggc
    42901 attccacaga ccataataat gttcggtatc aaccgcctct tcaccctgtt gataaggaac
    42961 agaaagccca ccgcccgcag aaatagcctg taaatcctga ccgaattcga ggacctgacg
    43021 caccatagca ccacacacct gttccagatg ggcataatca acgccagaac caatgtgcat
    43081 gtgaatgccg accagctgca gatgatgacg ttgtatcacg tccagtgcgg cgggcagatc
    43141 ggtgtaccag ataccgtgct tgctgttttc gccaccggta ttggtttttt ggctatgtcc
    43201 gtgaccaaac cccgggttaa cgcgcagcca tacccgatgc ccaggcgaaa cctggcccag
    43261 ttggtcgagc atatcaacag aacccgcatt caccggaatt tgcaattcac tgacgcgttc
    43321 aagcgtcgcc tgatcgataa catctgccgt aaaaacaata tcatcggggt gcgtttgcgg
    43381 attgtaaccc gccgccaacg cacgctctat ttcgcctaac gagacggaat ccactttcac
    43441 gccctgctca cgcattaagc gcaaaatatg aatattggaa caggctttct gtgcaaagcg
    43501 caccacatca aactgtttca gcgctgcaat ctgccgacga ataatttgcg catcgtagac
    43561 ccacaccggg cagccaaatt cagcgggcaa acgcagcaga ttttcggcgg tgagagcggt
    43621 atcggtgctg aacagtgaat gtggcatagc aaactccaga taagtgcttt tttatgatta
    43681 cgccacatca taaaaagaat aaaaaatatc gatttatgtc gagtctatgc aaaaatgata
    43741 tggattaccg gattgcgaga gagcgctaat ggccgccgtt aacttacgtc atattgaaat
    43801 ttttcatgcg gtaatgaccg ccggaagcct gactgaggca gcacacctgc tacacacctc
    43861 acagccaacc gtcagccgcg aactggcgcg ctttgagaag gtgatcgggc tgaaattgtt
    43921 tgagcgcgta cgtggacgat tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt
    43981 gcaacgatcc tggtacggac tggatcgcat tgtcagcgcc gcagaaagtc tgcgcgagtt
    44041 tcgccaggga gaactgtcta ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca
    44101 gctcctgcaa ccctttctgg cacgttatcc cgatgtcagc ttaaatatcg tgccccagga
    44161 atcaccgcta cttgaagagt ggctctcggc ccagcgtcat gatttaggac tcactgagac
    44221 actccatacg cctgcgggaa cagaacgtac cgaattactc tctttagatg aagtgtgtgt
    44281 gttacctccg ggtcatccac tggcggtaaa aaaggtatta acgccggatg attttcaggg
    44341 tgagaactac atcagccttt cccgtactga cagctatcgc cagttgctgg atcagctatt
    44401 tactgaacat caggttaaac gacgcatgat cgtagaaacc cacagcgccg cgtcagtgtg
    44461 cgcaatggta cgggcggggg taggtgtttc ggtggttaac ccgctcaccg cactggatta
    44521 tgcggcaagc ggtttagtgg tgcggcggtt cagtattgcg gttccgttca ccgtcagcct
    44581 gatccgcccc ctgcatcgcc cgtcatcagc gctggtgcag gcgtttagtg agcatttaca
    44641 agcgggttta ccgaaactgg tcacttctct tgacgcgatt ttgtcgtcag ctacgacagc
    44701 ataaaagcga cagcatcctc ggcatggatc gccgcggtat caaacacagg caaaacactg
    44761 agctcttctg gcaccagtaa accaatttct gtgcagccaa aaatgacgcc ctgtgcgccc
    44821 tgttctgcaa ggcgagcaat cacttgcgca taataagcgc gtgacgcttc ggtaaattgc
    44881 cccagacaca gttcttcaaa aataatctga ttaattttcg cccgttcatc cgcttcagga
    44941 ataagacaat tgatggaaaa ttgttccgtc agccgcccgc gataaaaatc ctgttccatg
    45001 gtgtaacgcg tacccagcag cgccacacga gtcattccgg ccccggtaat tgcacgtccg
    45061 gtggcatccg caatgtgtaa gaaaggcaga gtgcaacgtg actcaatggc atccgccact
    45121 ttatgcatcg tattggtaca taacacaata ccttctgcgc ccgcccgctg taagccaagc
    45181 gccgcctcag ccagaatgtc cccggtttta tcccattccc cgcgacgctg gcactcttct
    45241 atttcatgaa aatcgacgct atgtagcagc acttgcgcag agtgaagccc accaagccgc
    45301 tgtttaatgc cttcatttat cagacgatag taaggaatag tggattccca gctcattcct
    45361 cccagcaaac caattgtttt cattcctctc tcctgatgtg tgttaccgca acaaggctga
    45421 ataaggaacg agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg
    45481 gccggatgta cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc
    45541 ctgccatcag tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc
    45601 agaaagtgat gcctacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag
    45661 cgccaatgct atcaagtagt gtcaggaagg tcgcgccgat aatcatattc gacacccagt
    45721 ttgtcgtcgt tgagcaggtg atgccaaaat cgcggcattt cagcggctga atttcagagc
    45781 acaggatcca caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca
    45841 tgccaacaga gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc
    45901 aatagcccag caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt
    45961 tacgccctgc tttatctacc gtaaagaccg caatgaaggt cgcgaacata aaggtcagtc
    46021 ccacgaccag agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga
    46081 tacgcggcgc gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca
    46141 acataccgag aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc
    46201 cctgttttaa cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg
    46261 tatcgcgcag catacgcaac acttcttccg cctcaatatg acgccccttt tccgctagcc
    46321 agcgcgggct atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa
    46381 gaacccccaa cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg
    46441 ccagcacgat gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt
    46501 tttcacttgc catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa
    46561 tgcccagcac cacacgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta
    46621 tagaaccgag tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga
    46681 acgacagcca accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca
    46741 cccattcctg caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg
    46801 cgattacgcc gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa
    46861 acatattcat acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga
    46921 tagtaaccat tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac
    46981 tcacacaggt gtcaggtcgg aaacagcata aatatggatt aaattgctac gacatgtcgt
    47041 tatgtgatgg atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac
    47101 ctaattttac agcagataaa attcataaag ttcattaatt gataattgat atggattatt
    47161 tcataaccat gatatggatt atgatgatct acaggtataa aaaaccctgc catgcggcag
    47221 ggtcataaaa gtaagagaaa tgaattaacg cgccagccaa ccgccatcca cggcaatggt
    47281 ataaccattc acataatctg aagcgctgga ggcaaggaac actaccggcc ccatcaggtc
    47341 actcggcagt ccccaacggc cagctggaat gcggtcgaga atttccgcgc tacgttgttc
    47401 atctgcccgt agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac
    47461 attaatgttg tgtttagccc attcgttcgc catcaatcgc gtcacgccca taacaccgct
    47521 ttttgatgcg gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgcgat
    47581 attgataatc ttgccgccat tgccttgcgc gataaagtgt ttcgccgctg cctgagacat
    47641 gaagaatacg ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc
    47701 gagagcatct tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa
    47761 ctcagctacc gcgcgatcca gcagtgctgg aataccatca atctttcgca gatcggcggt
    47821 caggcttaaa aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac
    47881 gatgttaatg ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc
    47941 cagtccagta tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa
    48001 aatcataaca ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccaca
    48061 tggtccatat catcaaagac ctggttttca ccgaccatgc cccagataaa ggtataagct
    48121 ttggttccga cgccggaatg gatcgaccag ctcggggaga tcaccgcctg ctcgttatgc
    48181 atcacaatgt gacgcgtttc ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca
    48241 tccatattga aatagaaata aacttccatc cggcgctcgt gggtgtgaca cggcatggtg
    48301 ttccacaagt tacctggagc cagctccgtc agccccatac tcaattggca ggtttccagc
    48361 acatccggga caaaatattt gttaatcgtg cgacggttac tggtgaggtt atcccctaac
    48421 gtgactggag atacttcgtc cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt
    48481 acgcaattgt aataaaactt cgccggagtg gcggtatcat cactggcaaa gacaacttct
    48541 tttgcacctt tacccacata cagggcgtcg cggtgaccga tttcatagca ttgaccatcg
    48601 acagtaatcg taccggcacc gccaatattg ataacaccta actcgcgacg ttcaaggaaa
    48661 tagcttacgc cgagttgttt accaacttcc ccgccaacgg aaaccgtttt agttaccggc
    48721 ataatgccgc caacaataat acggtcaatg tggctgtaaa ccatggtgta ctcatcggtg
    48781 acaaatacct tttcaaccaa aaattcattg cgcagcccct gggtatccag tgtttttgcg
    48841 tgcgcactgt ggatgctctg tcttacgtcc acatcaacct ccgataatca gtgaagcgtg
    48901 aaaatagaaa acgatagtga aacactgttt cgttttctct ataaggggat gatatgccca
    48961 tgggataggg ttttcaatta aaaataaaac aatgtttcat ttttaagtta aggattaaaa
    49021 aaagtgccgt gtcgatcacg aatatgtcat gagaatggaa aaatcaggga gcccagaaaa
    49081 tgtatgtcga cttgccagcc atcattattc aacagattaa tgacggcaga tatttatcgg
    49141 taaggaggaa atgcagagct actcgtcacg ttcaatggtc aatgctacgc cctggccccc
    49201 gccgatacaa agcgttgcca gtcctttgcg ggcattacgt ttcaccattt catgaaccag
    49261 agaaaccagg attcggcaac cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc
    49321 attgacattg acccgacgct catcccattc aagcatcttg ccaaccgaaa gcgcctgtgc
    49381 agcaaacgct tcattagcct cgataagatc gacatcagcc aactgccagc ccacacgctc
    49441 caggcaacgg cgggtcgcat acaccggcgc aattcccatc aatgctggat ctacaccaac
    49501 gctggcaaat gcgcggatgc gggccagcac gggtaaattc aacgctcgtg ctttggcttc
    49561 gctcatcatc attaccgcag ctgcgccatc gtttatggat gatgcattac ccgctgtcac
    49621 agaaccgaga ctatcaaatg aaggatttaa acgggctaag ccttctgcac tggcgtcagt
    49681 gcgtggctgt tcatcggtat caataaccaa cgtctgacca ttactttggg tcattaccgg
    49741 gacgatctca tctttaaatc gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct
    49801 aagtgcgtaa gcatcctgca actgacggct gatgccatat tcgcgagcca gattttcggc
    49861 ggtgacacca atatgataat cattgaaggc atcccacaac ccatcatgca ccagactgtc
    49921 aaccaactgg ctattgccaa gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc
    49981 gcggctcatg ttttcctggc caccggcgat gacaatatca gcctcgccac actgtatcgc
    50041 ctgagtagcc agatgcagtg ccttaagccc ggaaccgcaa acgtcattaa tcgtgattgc
    50101 agaaacgcta ttaggaagac cacctttaat agccgattgc cttgccggat tctgccctgc
    50161 ccctgcagtc aacacctgac caagaattac ttcatccacc gcatatgcag gaacgccggt
    50221 acgttctatt aacgctttca cgaccagact accaagttcc acggcggaat gacccgctaa
    50281 cgcaccacga aagcagccga taggtgtccg taacgccccg acaatcacaa cgtctttcat
    50341 cacaacctcg caccaattaa cagtgcaata gtaaatgatt gttaattaca gttatcttaa
    50401 ttgtttaaaa aaagtgattt ttatcacaaa ggaaatatgc ctgagcagca gtcagagaca
    50461 taactggcac gtaaggtttg caaccactaa cccaccaata gaggggtaga tagggcgtta
    50521 atctcccata cttaacctgg tttatggtaa attgccctct attttgttta atttgtagat
    50581 gatacgttca gataatgtct aatatttggt caaaagaaga aactctgtgg agtttcgcgc
    50641 tctacggcac agccgttggt gcaggcacgc tcttccttcc tattcagtta ggttcggcag
    50701 gggcagtggt cctgtttatt actgctctgg tcgcctggcc tttaacatat tggccacata
    50761 aagccttatg ccagttcatc ctctcatcga aaacatcagc aggtgaaggg ataacgggcg
    50821 cagtaacaca ctactatggc aagaagattg gtaatctgat taccacgctg tacttcatcg
    50881 ccttttttgt cgtcgtgttg atatatgcag tggcaattac caactcactt acggaacagc
    50941 tggcaaagca tatggttatt gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat
    51001 taattctgaa tctcattttt ctgatgggac gtcatgccac tattcgggta atgggatttt
    51061 tggtattccc attgattgcc tatttcttat ttctttccat ttaccttgtc ggtagttggc
    51121 aacctgatct attaacaacc caggtagagt tcaatcagaa tacccttcac cagatatgga
    51181 tatcgattcc cgtgatggtt ttcgccttta gccatacgcc cattatttct acgtttgcca
    51241 tagacagacg tgaaaaatat ggcgaacacg ctatggataa atgcaaaaaa attatgaaag
    51301 tcgcttatct catcatctgc ataagtgtac tgttctttgt ctttagctgc ctgctttcta
    51361 ttccaccttc gtatattgaa gcggctaaag aagaaggggt caccatttta tcggcgcttt
    51421 ctatgctgcc gaacgcccca gcatggttgt caatttccgg gattattgtc gcagtagttg
    51481 cgatgtcgaa atcattcctg ggtacgtact ttggcgttat tgaaggtgcc acagaggtcg
    51541 tcaaaacaac attacagcag gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat
    51601 caattatgtt ggtatcgctg atcaccttca ttgtttgttg cattaacccg aacgcgattt
    51661 cgatgattta tgcgatcagc ggcccactca ttgccatgat acttttcatc atgcctacgc
    51721 tgtcaacgta tctcatcccg gcgcttaaac cctggcgttc catcggaaat ctgattacgc
    51781 tgatcgtggg tattctgtgc gtatcggtaa tgttctttag ctaatatcca ttgtgaatgg
    51841 ttcaaaattg ctacaatttt gaaccattcc atttttcata atgttcattt gttattattc
    51901 cgccctatca cttctctcat catatcctag aattatcagt tagttaaaca cacaaagaga
    51961 tctgaacatc ttaaataaga ttatttaaga tgcataactt agattcgcaa gatatcttct
    52021 ggtcattcaa aaacaatttg cgatatagat attctcatac catgagatag ttatctggac
    52081 cactctcaac catgcaagat taaatcagtg aaacataatc atatttaata acgagaaatg
    52141 catttttaaa tgtcttaatc agaattatag caaatacaga ttaatcgcaa tttttcccag
    52201 caacattaca aaatcatggt gtattgacat catcaataac caatgaaatt atgcaattat
    52261 atacggatag ggaggttctt aacatggggc tttgtagtcg ttataaaagt cttacatgca
    52321 atagttgctc aatgcattgc caaataatgc cagaagagtc accgcgtttg cagtattgcg
    52381 caaactcgtg tttttgtatg tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg
    52441 aaggtatctt aacaaaaaac cacaatgcga gattaagcgg atatattttt gtcgattttt
    52501 cagtaagttt tttacgtcta ttccttgaaa aagactggat tgactatctt gcaagtactg
    52561 acatggggat tgttttagtc agcgaccgca atatgcaatc gttagctaac tactggcgaa
    52621 agcacaactc tgcaatatct gctgtcatat ataatgatga tggtcttgat gttgccaatg
    52681 aaaagatcag acaactattt attggtcgtt atttatcatt taccagaggt aacacattaa
    52741 ctcagatgga attcaccata atggggtata tggtttctgg ttataaccca tatcagattg
    52801 ctgaagttct ggatatggat atccgtagca tctacgcgta caagcaacga atcgaaaaga
    52861 gaatgggtgg taaaataaac gagttattta ttcgttcaca ttcggtccaa cattaatact
    52921 caacgatctg ccagaatcat actctgctta tacgggcatc tattggaatg cccgtatttt
    52981 ataaataagc agtaacccca agagtaaaca acatagtatt taaaaccatc acacgaaaaa
    53041 ttcagaagca ttacgaaata tgaatttatc aacaatagtg gcataaatgt taaccatgtt
    53101 aatttatata aagttttacg ttgcgccatt
//
LOCUS       DAZGBC010000013        50821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGBC010000013 DAZGBC010000000
VERSION     DAZGBC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.013"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..575
                     /gene="elfA"
                     /locus_tag="ACHK1J_000794"
     CDS             36..575
                     /gene="elfA"
                     /locus_tag="ACHK1J_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910117.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            658..1359
                     /gene="elfD"
                     /locus_tag="ACHK1J_000795"
     CDS             658..1359
                     /gene="elfD"
                     /locus_tag="ACHK1J_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7910118.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1384..3984
                     /gene="elfC"
                     /locus_tag="ACHK1J_000796"
                     /pseudo
     CDS             1384..3984
                     /gene="elfC"
                     /locus_tag="ACHK1J_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3975..5045
                     /gene="elfG"
                     /locus_tag="ACHK1J_000797"
     CDS             3975..5045
                     /gene="elfG"
                     /locus_tag="ACHK1J_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910119.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRNGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARINVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5057..5599
                     /gene="ycbU"
                     /locus_tag="ACHK1J_000798"
     CDS             5057..5599
                     /gene="ycbU"
                     /locus_tag="ACHK1J_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910120.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANIVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5607..6122
                     /gene="ycbV"
                     /locus_tag="ACHK1J_000799"
     CDS             5607..6122
                     /gene="ycbV"
                     /locus_tag="ACHK1J_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910121.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6088..6825
                     /gene="ycbF"
                     /locus_tag="ACHK1J_000800"
     CDS             6088..6825
                     /gene="ycbF"
                     /locus_tag="ACHK1J_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO7910122.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSLRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6936..7946
                     /gene="pyrD"
                     /locus_tag="ACHK1J_000801"
     CDS             6936..7946
                     /gene="pyrD"
                     /locus_tag="ACHK1J_000801"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO7910123.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8120..8662
                     /gene="zapC"
                     /locus_tag="ACHK1J_000802"
     CDS             8120..8662
                     /gene="zapC"
                     /locus_tag="ACHK1J_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO7910124.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8659..9768)
                     /gene="ycbX"
                     /locus_tag="ACHK1J_000803"
     CDS             complement(8659..9768)
                     /gene="ycbX"
                     /locus_tag="ACHK1J_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO7910125.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10012..12120
                     /gene="rlmKL"
                     /locus_tag="ACHK1J_000804"
     CDS             10012..12120
                     /gene="rlmKL"
                     /locus_tag="ACHK1J_000804"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO7910126.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12132..14039
                     /gene="uup"
                     /locus_tag="ACHK1J_000805"
     CDS             12132..14039
                     /gene="uup"
                     /locus_tag="ACHK1J_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7910127.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14169..15422
                     /gene="pqiA"
                     /locus_tag="ACHK1J_000806"
     CDS             14169..15422
                     /gene="pqiA"
                     /locus_tag="ACHK1J_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO7910128.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15427..17067
                     /gene="pqiB"
                     /locus_tag="ACHK1J_000807"
     CDS             15427..17067
                     /gene="pqiB"
                     /locus_tag="ACHK1J_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO7910129.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17064..17627
                     /gene="pqiC"
                     /locus_tag="ACHK1J_000808"
     CDS             17064..17627
                     /gene="pqiC"
                     /locus_tag="ACHK1J_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO7910130.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17883..18050
                     /gene="rmf"
                     /locus_tag="ACHK1J_000809"
     CDS             17883..18050
                     /gene="rmf"
                     /locus_tag="ACHK1J_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO7910131.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18120..18638)
                     /gene="fabA"
                     /locus_tag="ACHK1J_000810"
     CDS             complement(18120..18638)
                     /gene="fabA"
                     /locus_tag="ACHK1J_000810"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO7910132.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFNKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18707..20467)
                     /gene="ycbZ"
                     /locus_tag="ACHK1J_000811"
     CDS             complement(18707..20467)
                     /gene="ycbZ"
                     /locus_tag="ACHK1J_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7910133.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20653..21105
                     /gene="matP"
                     /locus_tag="ACHK1J_000812"
     CDS             20653..21105
                     /gene="matP"
                     /locus_tag="ACHK1J_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO7910134.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21181..22236)
                     /gene="ompA"
                     /locus_tag="ACHK1J_000813"
     CDS             complement(21181..22236)
                     /gene="ompA"
                     /locus_tag="ACHK1J_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO7910135.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKAHNNVTGESEKNHDTGVSPVFAGGVEWAITPE
                     IATRLEYQWTNNIGDAHTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTK
                     HFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQ
                     GLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRR
                     VEIEVKGIKDVVTQPQA"
     gene            complement(22593..23102)
                     /gene="sulA"
                     /locus_tag="ACHK1J_000814"
     CDS             complement(22593..23102)
                     /gene="sulA"
                     /locus_tag="ACHK1J_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO7910136.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23321..23950
                     /gene="sxy"
                     /locus_tag="ACHK1J_000815"
     CDS             23321..23950
                     /gene="sxy"
                     /locus_tag="ACHK1J_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO7910137.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23913..26075)
                     /gene="yccS"
                     /locus_tag="ACHK1J_000816"
     CDS             complement(23913..26075)
                     /gene="yccS"
                     /locus_tag="ACHK1J_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO7910138.1"
                     /translation="MAYMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26085..26531)
                     /gene="yccF"
                     /locus_tag="ACHK1J_000817"
     CDS             complement(26085..26531)
                     /gene="yccF"
                     /locus_tag="ACHK1J_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO7910139.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26654..28708
                     /gene="helD"
                     /locus_tag="ACHK1J_000818"
     CDS             26654..28708
                     /gene="helD"
                     /locus_tag="ACHK1J_000818"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO7910140.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28740..29198)
                     /gene="mgsA"
                     /locus_tag="ACHK1J_000819"
     CDS             complement(28740..29198)
                     /gene="mgsA"
                     /locus_tag="ACHK1J_000819"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO7910141.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29294..29956)
                     /gene="yccT"
                     /locus_tag="ACHK1J_000820"
     CDS             complement(29294..29956)
                     /gene="yccT"
                     /locus_tag="ACHK1J_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO7910142.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30129..30542
                     /gene="yccU"
                     /locus_tag="ACHK1J_000821"
     CDS             30129..30542
                     /gene="yccU"
                     /locus_tag="ACHK1J_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO7910143.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30587..30904)
                     /gene="hspQ"
                     /locus_tag="ACHK1J_000822"
     CDS             complement(30587..30904)
                     /gene="hspQ"
                     /locus_tag="ACHK1J_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO7910144.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30962..32152)
                     /gene="rlmI"
                     /locus_tag="ACHK1J_000823"
     CDS             complement(30962..32152)
                     /gene="rlmI"
                     /locus_tag="ACHK1J_000823"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO7910145.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCNQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32247..32525
                     /gene="yccX"
                     /locus_tag="ACHK1J_000824"
     CDS             32247..32525
                     /gene="yccX"
                     /locus_tag="ACHK1J_000824"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO7910146.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32522..32851)
                     /gene="tusE"
                     /locus_tag="ACHK1J_000825"
     CDS             complement(32522..32851)
                     /gene="tusE"
                     /locus_tag="ACHK1J_000825"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO7910147.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32942..33601)
                     /gene="yccA"
                     /locus_tag="ACHK1J_000826"
     CDS             complement(32942..33601)
                     /gene="yccA"
                     /locus_tag="ACHK1J_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO7910148.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34009..35028)
                     /locus_tag="ACHK1J_000827"
     CDS             complement(34009..35028)
                     /locus_tag="ACHK1J_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7910149.1"
                     /translation="MGRPRKNKKDNALPPRVRSNGYSYVWKPEGSTKTIGLGRVRETS
                     IAKVWQNYELEKAKRHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     VLADNVKIEQVRIFMDKRGIESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKHAA
                     EKKSGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(34997..35248)
                     /locus_tag="ACHK1J_000828"
     CDS             complement(34997..35248)
                     /locus_tag="ACHK1J_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO7910150.1"
                     /translation="MYELTLSPAEIQEITRYERYTKQQQQLRLHGIPFVPGPKNEPIV
                     LRSDVPRGLTAIPKVSELVSADPDFEALNNGKTKKKQKR"
     gene            complement(35315..37753)
                     /locus_tag="ACHK1J_000829"
     CDS             complement(35315..37753)
                     /locus_tag="ACHK1J_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7910151.1"
                     /translation="MSTDKEEFSLYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINGGLA
                     AKCKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQQETEQNG
                     QKAEQNSPNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCGAVMGDATYQET
                     FNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANPLIKVNGHREITS
                     TSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVDIS
                     TIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVILR
                     RSYERQEIPCPWRYTNDRDVRTIVALGLVMDFDARSVITFEGERHNALHDARYQAKYV
                     SAIWQKLFPNQADF"
     gene            complement(37846..38034)
                     /locus_tag="ACHK1J_000830"
     CDS             complement(37846..38034)
                     /locus_tag="ACHK1J_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7910152.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(38031..38219)
                     /gene="dicB"
                     /locus_tag="ACHK1J_000831"
     CDS             complement(38031..38219)
                     /gene="dicB"
                     /locus_tag="ACHK1J_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7910153.1"
                     /translation="METLLPNVNTSEGCFDIGILLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(38230..39084)
                     /locus_tag="ACHK1J_000832"
     CDS             complement(38230..39084)
                     /locus_tag="ACHK1J_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7910154.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            complement(39603..39755)
                     /locus_tag="ACHK1J_000833"
     CDS             complement(39603..39755)
                     /locus_tag="ACHK1J_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7910155.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            40030..40332
                     /locus_tag="ACHK1J_000834"
     CDS             40030..40332
                     /locus_tag="ACHK1J_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO7910156.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            40335..40694
                     /locus_tag="ACHK1J_000835"
     CDS             40335..40694
                     /locus_tag="ACHK1J_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7910157.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(40741..41133)
                     /locus_tag="ACHK1J_000836"
     CDS             complement(40741..41133)
                     /locus_tag="ACHK1J_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7910158.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            41260..41520
                     /locus_tag="ACHK1J_000837"
     CDS             41260..41520
                     /locus_tag="ACHK1J_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7910159.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANEQAAAGVDS"
     gene            41517..41954
                     /locus_tag="ACHK1J_000838"
     CDS             41517..41954
                     /locus_tag="ACHK1J_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7910160.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            42041..43051
                     /locus_tag="ACHK1J_000839"
     CDS             42041..43051
                     /locus_tag="ACHK1J_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO7910161.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAERKVFTQVQWEQKFARHVQHVRAQVKPVSKGGSHAASGGTAS
                     RAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            43044..43505
                     /locus_tag="ACHK1J_000840"
     CDS             43044..43505
                     /locus_tag="ACHK1J_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7910162.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKYPKGPTPIEWLKQEYERRKAAGFI"
     gene            43539..44300
                     /locus_tag="ACHK1J_000841"
     CDS             43539..44300
                     /locus_tag="ACHK1J_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7910163.1"
                     /translation="METVFDALKAMGKASSVGLAERLDISREEVLNELWELKRNGVVD
                     KAGHIWFLAGEGESGVTEEQPEQSEVPDVLTGEVEQKVTADMMIEFIGQEGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAVPVTENDGKA
                     FPQPAGVALPVQESATQEDIKTETVADIVQSLPSFTETQADELIFLSLCRANLALRRA
                     KSDVQKWERVCAALRELNKHRDILRDITATRELLR"
     gene            44333..44614
                     /locus_tag="ACHK1J_000842"
     CDS             44333..44614
                     /locus_tag="ACHK1J_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7910164.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            44611..44916
                     /locus_tag="ACHK1J_000843"
     CDS             44611..44916
                     /locus_tag="ACHK1J_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO7910165.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            44903..45553
                     /locus_tag="ACHK1J_000844"
     CDS             44903..45553
                     /locus_tag="ACHK1J_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7910166.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLSEVRALGVDA
                     AIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLIGKVEMTEWLVDFAAQLRKGVQS"
     gene            45550..45879
                     /locus_tag="ACHK1J_000845"
     CDS             45550..45879
                     /locus_tag="ACHK1J_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910167.1"
                     /translation="MRASISYVDDCHLSVRVDEIVSSVPTFPTKNAAVNAGAPFGWRT
                     AVRIERRFENVWVVGKKCFQSDRSAGLNFEAYRFPFLRWEKEGGITKCPILSVRRFKQ
                     EATSEQD"
     gene            45866..46210
                     /locus_tag="ACHK1J_000846"
     CDS             45866..46210
                     /locus_tag="ACHK1J_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7910168.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVGIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            46212..46565
                     /locus_tag="ACHK1J_000847"
     CDS             46212..46565
                     /locus_tag="ACHK1J_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910169.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAKP
                     VASCIIEDGGMCVDGFGEYVGHSLPDGTHQLYAAPPVPSKLPREYRNGWPLAYSDYAE
                     GWNDCREAMLQGDKS"
     gene            46562..46924
                     /locus_tag="ACHK1J_000848"
     CDS             46562..46924
                     /locus_tag="ACHK1J_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7910170.1"
                     /translation="MINRIKLEHILEYARQQKHTGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPDTKTAVLVAREFDRKGDWRMKWATYIPGHPDANDGWVVPGAS
                     WIPSHWMPLPEPPKEVRQ"
     gene            complement(47127..47426)
                     /locus_tag="ACHK1J_000849"
     CDS             complement(47127..47426)
                     /locus_tag="ACHK1J_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7910171.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNIIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(47432..47689)
                     /locus_tag="ACHK1J_000850"
     CDS             complement(47432..47689)
                     /locus_tag="ACHK1J_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO7910172.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRVAIRSGK"
     gene            47825..48103
                     /locus_tag="ACHK1J_000851"
     CDS             47825..48103
                     /locus_tag="ACHK1J_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910173.1"
                     /translation="MAQDTKLHNSDNSTAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            48105..49163
                     /locus_tag="ACHK1J_000852"
     CDS             48105..49163
                     /locus_tag="ACHK1J_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7910174.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISSTQERE
                     LSLAELSWWAVCNQVVDALPEAVVRRSLGLPAEKIRSVYRESDIIPGEQAATSILKQR
                     TKNIALPLHVHQQQNPPQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            49164..49544
                     /locus_tag="ACHK1J_000853"
     CDS             49164..49544
                     /locus_tag="ACHK1J_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7910175.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVR
                     GKPVSGGRLGVKIYPIMLEGQVKK"
     gene            49541..50362
                     /locus_tag="ACHK1J_000854"
     CDS             49541..50362
                     /locus_tag="ACHK1J_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7910176.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDCVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCSCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVSKFDIEEAWAERQLKKVTR"
     gene            50589..50786
                     /locus_tag="ACHK1J_000855"
     CDS             50589..50786
                     /locus_tag="ACHK1J_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7910177.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNMYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
BASE COUNT        13119 a        11899 c        13258 g        12545 t
ORIGIN      
        1 attatcatat ttacgaagag atcaggatga taacgatgaa aaaaagtgta ttgacggcgt
       61 ttataactgt ggtatgtgca acgtccagtg ttatggcagc tgatgataat gctatcacgg
      121 atggttcagt aacatttaat ggtaaagtta ttgctccagc ttgtaccctg gtcgctgcga
      181 cgaaagattc cgtggtgact ttgccagatg ttagtgccac gaagttacaa accaatggtc
      241 aggtttctgg cgtgcaaact gatgtgccaa ttgaattaaa agattgtgat actaccgtaa
      301 caaaaaatgc aacgttcacc tttaatggca ctgcggatac tactcagatt acagcgtttg
      361 ctaaccaggc ctcatctgat gctgctacaa acgtggccct gcaaatgtat atgaatgatg
      421 gtacaacggc catcacgcca gacacagaaa ccgggaatat tttgttgcaa gatggagatc
      481 agacgttgac ttttaaagtt gattatatcg ctacggggaa agcgacttct ggtaatgtga
      541 atgcggtaac aaatttccat attaactatt attaatagaa ctcattaatt gttttattaa
      601 ttagtacccc tccagtgttc tggaggggat attcatattt tttaagagtg actatttatg
      661 aaaacttgca taacaaaggg aattgtgacc gtaagtttaa cggcaatatt actcagctgc
      721 tcatccgcat gggcggcagg taaaggcggg attggacttg cagcaacacg tcttgtttat
      781 tcagaaggtg aagagcaaat ttcactgggt gtacgtaaca ccagtccgga tgttccttat
      841 cttattcagt catgggtgat gaccccagat aataaaaaat cagcagactt tattattaca
      901 ccaccgctat ttgtgctgaa tccggctaat gagaatctgt tacgcattat gtacattggt
      961 gcgccgttgg cgaaagacag agaaactctt ttcttcacta gcgtacgggc agtcccttca
     1021 acaacgaagc gggaagaagg caataccctg aagattgcca cacaaagcgt catcaaactt
     1081 ttctggcgac caaaaggttt agcgtatccc ttaggcgagg ctccggcgaa actgcgttgc
     1141 acttcgtcag ctgacatggt tacggtcagt aacccaacac cttatttcat taccctgaca
     1201 gacctgaaaa taggtggaaa ggtagttaaa aatcaaatga tttccccctt tgataaatac
     1261 caattctctc tgccaaaggg tgccaaaaat agcagcgtaa cgtatcgaac catcaatgac
     1321 tacggggcgg aaacgccgca actcaactgt aaatcgtaat tcgtcttcag ttaagagagc
     1381 gagatgtata gaactcaccg acaacacagc ctgttaagct ctggtggcgt gccatcgttt
     1441 attggtgggc tggtggtgtt tgtgtcggca gcgttcaatg cacaagctga aacctggttc
     1501 gatccagcct ttttcaaaga tgatccctca atggtggccg atttgtctcg tttcgaaaaa
     1561 ggacaaaaaa taacgccagg ggtttatcgt gtcgatattg ttctgaatca gacaattgta
     1621 gatacgcgca acgtcaattt tgttgagata acgccagaga aggggattgc cgcctgtttg
     1681 acgaccgaaa gcctggatgc aatgggcgtg aatactgatg catttccggc ttttaaacaa
     1741 ctggacaaac aagcgtgtgc gctattggcg gagattattc cggatgccag cgtaactttt
     1801 aatgtgaata aactccgtct ggaaatttca gtaccgcaaa ttgccatcaa aagtaatgct
     1861 cgtggttatg tcccccctga acgttgggat gaagggatca acgcgctatt actgggatat
     1921 tcatttagcg gggctaacag tattcatagc agcgcagaca gtgattctgg cgacagttat
     1981 tttctgaatt taaacagtgg cgttaattta ggcccatgga gattgcgcaa caattcaaca
     2041 tggagtcgta gtagtggcca aaccgcagaa tggaagaatc tcagcagcta tttgcaacgg
     2101 gcggttattc cactaaaagg cgaactgacc gtaggtgatg attatactgc aggcgatttt
     2161 ttcgatagcg ccagctttcg tggtgtgcag ctggcatcag atgacaacat gctgccagac
     2221 agcctgaaag ggtttgcgcc tgtagtgcgt ggtatcgcca aaagcaatgc ccagataacg
     2281 attaagcaaa atggttacac catttaccaa acttatgtat cgcctggtgc ttttgaaatt
     2341 agtgatctct attccacgtc gtcgagcggt gatttgttgg ttgaaatcaa agaagcggac
     2401 ggcagcgtca atagctatag cgtgcccttt tccagcgtgc cattactcca gcgtcagggg
     2461 cgaatcaaat acgcggtgac actggcgaaa tacaggacca atagtaatga acagcaggag
     2521 agcaaatttg cccaggtcac gttgcagtgg ggcggaccgt ggggaacgac ctggtatggt
     2581 ggtggacaat atgctgaata ttaccgtgct gccatgtttg gtctgggatt taacctcggc
     2641 gatttcggag caatttcgtt cgatgcgacc caggcgaaga gtacgctggc agaccaaagc
     2701 gaacataaag gtcagtcata tcgttttctg tatgccaaaa cgctcaacca attgggcact
     2761 aattttcaat tgatgggcta tcgctattcg acgtcgggtt tctacaccct ttccgacacc
     2821 atgtataaac atatggatgg ctacgaattt aatgacggtg atgatgaaga tacgccgatg
     2881 tggtcgcgtt attacaattt gttttacacc aaacgtggca aactgcaggt caatatctcc
     2941 cagcaattag gcgagtacgg ttcgttttat ttaagtggta gccagcaaac ttactggcat
     3001 accgatcaac aggatcggct attacagttt ggctataaca cgcaaattaa agatctctcg
     3061 ctggggatct cctggaacta cagtaagtcc cgtggtcaac ctgatgctga tcaggtgttt
     3121 gcactaaatt tttccctgcc gctcaatctg ttgctcccca gaagtaatga tagctatacc
     3181 aggaaaaaaa attacgcctg gatgacctct aacaccagta tcgataacga agggcacatt
     3241 acacaaaacc tgggtttaac ggagacacta ctcgatgatg gtaacctgag ctacagcgtg
     3301 caacagggat ataacagcga ggggaaaacg gctaatggta gcgccagcat agactacaaa
     3361 ggggcgtttg cagatgcccg agtgggctac aactacagcg ataacggcag tcaacaacaa
     3421 ctgaactacg ctctttcagg cagtttagtt gcccattcac agggcattac cctggggcaa
     3481 tcgctggggg aaactaacgt gctgattgca gcaccaggcg cagagaatac tcgtgtggcg
     3541 aacagcaccg ggctgaaaac tgactggcgc ggatataccg ttgttcctta tgccacttct
     3601 tatcgtgaaa attgaatcgc acttgatgcg gcgtcgttaa aacgtaacgt ggatcttgaa
     3661 aatgcagtag tcaacgtggt tcccaccaaa ggggcgttgg ttctggcgga gttcaatgcc
     3721 catgcgggag caagggtatt aatgaaaaca acaaagcagg gtataccgct gcgttttggc
     3781 gcgatagcga cgctggacgg ggtacaggct aatagcggca taattgatga tgatggctcg
     3841 ctctatatgg cgggtttacc ggcgaaggga acaataaccg tacgctgggg cgaagcaccc
     3901 gatcaaattt gtcatatcag ttacgagctt accgaacaac aaattaactc tgcgattacg
     3961 cgaatggatg ccatatgcag ataatctttg gagaaaaatg cgtgtcatta ctacgactat
     4021 tttttgccgc cgtcttaatg ctatggtgcg ctcaaaccgc tgcttatagc gggcagtgtc
     4081 ataccactca ggggaatccg tatattggcg tcaattttgg cgttaaaacc ctggaggaag
     4141 aagaaaatac tgcaggggta gttaaagaca aattttatca gtggaacgaa tcgaatgatt
     4201 attatgtttc ctgtgattgc gataaagaca atgtcagaaa tggtcgatgg gcattcgccg
     4261 cggattcacc gttagtctat ttaggcgaca actggtacaa aattaatgac tatcttgccg
     4321 ccaaagtttt attgcaggtt aaaggcagtt ctcctactgc ggttcctttc gaaaacgtgg
     4381 gcacaggggg ggatacccga tggcatattt gcgaccctgg cggtcaacgt ttaggcggcc
     4441 agggggctag cggtaatagc ggtagctttt ccctgaaaat attgcagccg ttcgttggct
     4501 cggtcgtcat tcctcctatg gcgctggcgc gattatttga atgctacaac atacctgcag
     4561 gtgattcctg cacgactaca ggtacaccgg ttttagtgta ttacctgtct ggtacgatca
     4621 attcacttgg ctcatgttcc gtcaatgccg gagaaacaat cgaggttgat ctgggcgacg
     4681 tatttgcggc taactttcgt gttgtagggc ataagcctct tggggccaga acggcagaac
     4741 ttgcaattcc ggtcaggtgt aacacgggaa acgcggggtt agttaacgtc aacctgagtc
     4801 tgactgcaac ctcagacccc agctatcccc aggcgattaa gacgtcacgt ccaggcgtgg
     4861 gcgtggtggt gaccgatagc cagaacaaca ttatttcccc ggctggtgga acactaccgc
     4921 tctctattcc tgatgatgca gacagtatcg cgcgaataaa tgtctatcca gtcagcacga
     4981 caggtgtacc accagaaacc gggcgatttg aagccacggc aacggtgaga ataaattttg
     5041 attaacgcgt gaacgtatga agaaaaaaac gatatttcag tgcgttatct tgttctttag
     5101 cattcttaac atccatgtcg ggatggctgg gcctgaacaa gttagtatgc atatttatgg
     5161 gaatgtggtc gatcagggct gtgatgtcgc caccaaaagt gcattacaaa atattcatat
     5221 tggtgatttt aatatcagtg attttcaggc cgcgaatatc gtaagcactg ctgctgattt
     5281 gaatatcgat atcaccggtt gtgccgctgg tattactggc gcggacgtcc tttttagcgg
     5341 cgaggctgac acccttgcgc cgacactgct caaactaact gacacaggcg gaagcggtgg
     5401 tatggcaacg gggattgccg tgcaaattct tgatgcgcaa agtcagcaag aaatcccgct
     5461 caatcaggtc cagcctctta cgcccttaaa agccggggat aacacactca aatatcaact
     5521 tcgttataag tccacaaagg cgggagcaac gggcggtaat gcgacggcgg ttctctattt
     5581 tgatctggtt taccagtgaa ggagtgatgt tgaaacggat aatctggatt ctgttcttac
     5641 tgggattaac ggggggttgt gagctatttg cccatgatgg cacggtcaac attagcggat
     5701 cgtttcgccg taatacatgc gtgctggcac aggatagcaa gcaaatcaat gtgcagttag
     5761 gcgatgtctc actgacacgt ttttctcatg ggaattatgg cccggagaaa tctttcatca
     5821 ttaatttgca ggattgcgga acagatgtca gcacggttga cgtcaccttt tcaggaaccc
     5881 ccgacggagt gcagagcgaa atgttgtcca ttgaaagtgg tacggatgca gccagcgggc
     5941 tggcaattgc gattctggat gatgcgaaga tattgattcc gcttaaccag gcgagtaaag
     6001 actacagcct acatagcggt aaggtgccat taacttttta tgcgcaattg cgacctgtca
     6061 atagcgatgt gcagtcgggt aaagtgaatg ccagcgcgac atttgtactt cattatgact
     6121 aatacgtgga acagattggc gctcttgatt ttcgccgttt tatcgctgct ggtggcgggt
     6181 gaattgcagg caggagtggt ggtcggcgga acgcgattta tctttccggc agacagagaa
     6241 tcgatatcta ttttactgac taataccagt caggaatcct ggcttattaa cagtaaaatc
     6301 aaccgcccaa cgcgttgggc cgggggtgaa gcgtcgacag tgccggcacc attactggcc
     6361 gctccgccac ttattctcct gaagcccggt acgacaggca cgttgcgctt gctgagaacg
     6421 gaaagcgaca tcttgcctgt ggatcgcgaa acgctatttg agttaagcat tgccagcgtg
     6481 ccatccggca aagttgaaaa tcagagcgta aaagtggcga tgcgctcggt atttaaactg
     6541 ttctggcgac ccgaaggttt gccgggcgac ccgctggaag cttatcaaca attacgctgg
     6601 acacggaatt cgcagggtgt acaactcact aacccaacgc cttactacat taacctgatt
     6661 caggtaagcg taaatggtaa agcgttaagt aatgtgggag ttgtgccgcc taaaagcctg
     6721 cgtcagacaa gctggtgtca ggcaatagca ccgtgtcatg ttgcctggcg ggcgataaat
     6781 gattacggcg ggttgagtgc aaagaaggag caaaatctgc cctgaaacag gttcggaaaa
     6841 cgtttgcgtt ttttttgccg caggtcaatt cccttttggt ccgaactcgc acataatacg
     6901 cccccggttt gcacaccggg aatccaggag agttcatgta ctaccccttc gttcgtaaag
     6961 cccttttcca gctcgatcca gagcgcgctc atgagtttac ttttcagcaa ttacgccgca
     7021 ttaccggaac accgtttgaa gcactggtgc ggcagaaagt gcctgcgaaa cctgttaact
     7081 gcatgggcct gacgtttaaa aatccgcttg gtctggcagc cggtcttgat aaagacgggg
     7141 agtgcattga cgcgttaggc gcgatgggat ttggatcgat cgagatcggt accgtcacgc
     7201 cacgtccaca gccaggtaat gacaagccgc gtctctttcg tctggtagat gccgaaggtt
     7261 tgatcaaccg tatgggcttt aataatcttg gcgttgataa cctcgtagag aacgtaaaaa
     7321 aagcccatta tgacggcgtc ctgggtatta acatcggcaa aaataaagat acgccagtag
     7381 agcagggcaa agatgactat ctgatttgta tggaaaaaat ctatgcctat gcgggatata
     7441 tcgccatcaa tatttcatcg ccaaataccc caggattacg cacactgcaa tatggtgaag
     7501 cgctggatga tctcttaact gcgattaaaa ataaacaaaa tgatttgcaa gcgatgcacc
     7561 ataaatatgt gccgatcgca gtgaagatcg cgccggatct ttctgaagaa gaattgatcc
     7621 aggttgccga tagtttagtt cgccataata ttgatggcgt gattgccacc aataccacac
     7681 tcgatcgttc tctggttcag ggaatgaaaa attgcgatca aaccggtggc ttaagtggtc
     7741 gtccgcttca gttaaaaagc accgaaatta ttcgccgctt gtcactggaa ttaaacggtc
     7801 gcttaccgat catcggtgtt ggcggcatcg actcggttat cgctgcgcgt gaaaagattg
     7861 ctgcgggtgc ctcactggtg caaatttatt ctggttttat ttttaaaggt ccgccgctga
     7921 ttaaagaaat cgttacccat atctaattat ttcttcgaat tcctctttat aagataatca
     7981 gggctttatt ttcagccctg gttgttttat attcatcact gttgcttatt tagacatttt
     8041 gtacttttat tgttgaggtt attaagcgaa gcgacaatgg attgtgttgc tgcggtttta
     8101 taaggttggg gagaggcaga tgcgaattaa accagacgat aactggcgtt ggtattacga
     8161 tgaagagcac gatcgaatga tgctcgattt agccaatggt atgctatttc gctcacgttt
     8221 tgcgcgcaaa atgttgactc cagatgcctt ttctcccgca ggcttttgcg ttgacgacgc
     8281 cgcgctctat ttctcttttg aagaaaagtg ccgcgatttt aatttatcta aagaacaaaa
     8341 agccgaactg gtgctgaatg cactggtagc aattcgctat ctcaaaccgc aaatgccgaa
     8401 aagctggcat tttgtttccc atggtgaaat gtgggtgccc atgccggggg atgcggcctg
     8461 cgtttggttg agcgacacta acgagcaagt gaatttactg gtcgttgaat ctggcgagaa
     8521 tgccgcgcta tgcctgctgg cacaaccctg cgttgttatt gcgggtcgcg cgatgcagtt
     8581 gggcgacgcc attaaaatca tgaacgacag gctgaaaccg caggttaatg ttgacagctt
     8641 cagcctcgaa caggcagtct aacgcgccaa cttaagtgca gtcttcggta cacagctaca
     8701 gcaaagaatg gtgccatcat cgcccattgc tgattttttc agcggcgtga cttcgccttc
     8761 taaaagctga acacggcaac ttccacaaat gcccgcgcgg caagaataag ggatacgaat
     8821 tccctgattt tctaattgct ccagcaacac ctgttggtta tttccacgaa atgcctgtcc
     8881 ctgccagtca atatctacat tggcgtccgg ttgttgcgtg atgttggcag tatcatcagc
     8941 ggcagctgcg ccgtaaattt ttgccggagc cgttgtcaga atttccacct catcgccaac
     9001 gcgaatcacg ccgctattac gggcaattaa attctgacca aaatcgacat cgccgttatc
     9061 ctgggcagtg cggaaagatt gcaatgtttt taatggttcg cctgccggat gtttttgccc
     9121 tttttctggg ctgacggtgg tgaaaataca gcggctacaa ggtttaacca catcaaacac
     9181 cacatcacca atgcgaatca ctttccaact atcttcttcc caggctgacg cgccggaaac
     9241 caccagattg gggcggaatt gctccatttt tacactggcc ggacaacgtt gttggagatc
     9301 acgtaacgag gcttcgttag caagaaggta aggatagcca tcagcaaatg acaggggtac
     9361 agtgttgtgg cgtttcacgc gccgggtcat ttgtggcccc acccagcgta attgcacttc
     9421 gcgggagaaa aatccactta accatttgtt gatcgcttct ggcgcaattc gcgcggtaaa
     9481 atgtgtgccc caaacttcgg ttggtgcgtc ttgtgtggcg aaatcagcaa aacgaacata
     9541 tgcgctactg ccatctggtg cggtgagatg caagccatca tgcacaggtg aaggagtaaa
     9601 ccgtaccatc tggggaaact ggcgagcggt aataaacgta ccgtcaggtt ccgtgatcat
     9661 aaagatgcga tcgaaggcca gaccactgac atcagccaga gcatgtgtaa gaccaatgcc
     9721 gcgcatcgat ttaacaggat gaataaaaag ccgggttaat gtcgccacgg tgtggtcctc
     9781 aaatgaaaat aagccctcaa ctttatgaca tgacgcgctt attagctata atgcgcaaca
     9841 attttcttag cgcctgtccc attaggctat tttatttgtc agtttggccc tgggttgtgc
     9901 tcgaaatcct cacgtacttg tgtacgctcc ggtttctccg cgctgtccat gtccagactg
     9961 actgcaacaa ttacgcctac tgcggtaggc tcttagagta aaagtgacga tatgaattct
    10021 ctgtttgcca gtacggcccg tgggctggaa gagctgttaa aaactgaact ggaaaacctg
    10081 ggggccgttg aatgccaggt ggttcagggt ggggtccatt tcaagggcga cacacggctt
    10141 gtttaccaga gcctgatgtg gagccgcctg gcctcgcgta ttatgttgcc gctgggcgag
    10201 tgtaaggttt acagcgattt agacctctat ctcggtgttc aggcgatcaa ctggacagag
    10261 atgtttaatc ctggcgcgac cttcgctgtc cacttcagtg gtttgaatga cacaatccgc
    10321 aacagtcagt acggcgcgat gaaagtgaaa gacgcaatcg tcgatgcttt cacgcggaaa
    10381 aatctgccgc gtccaaatgt tgatcgcgat gcgccggata tccgcgttaa cgtctggctg
    10441 cataaagaaa ccgccagtat cgctcttgat ctgagtggtg atggtttaca tctgcgtggc
    10501 tatcgcgatc gtgctggtat tgcgccgatc aaagaaaccc tggcagccgc tattgtgatg
    10561 cgatccggct ggcagccagg aacaccgctg ctcgatccga tgtgtggttc cggtacgttg
    10621 ctgattgaag cggcgatgct ggcgaccgat cgcgcaccag gcttgcaccg tgggcgttgg
    10681 ggctttagcg gctgggcgca gcatgatgaa gctatctggc aggaagtgaa agcagaagcg
    10741 caaactcgcg cccgtaaagg cctggctgag tatagctctc atttttacgg ttcggacagc
    10801 gacgcacggg tgattcaacg tgcgcgcact aacgcccgtc ttgcggggat tggtgaactg
    10861 atcacctttg aggtgaaaga tgtcgcgcaa ctgaccaatc cgctgccgaa agggccgtac
    10921 ggcacagtgt tgagcaaccc gccatacggg gaacgtctgg acagcgaacc ggcgctgatt
    10981 gcgctgcata gcctgctggg tcggatcatg aaaaaccagt ttggtggctg gaatctctct
    11041 ttgtttagtg cctcgccgga tctgctaagc tgcttgcagc tgcgtgcaga caaacagtac
    11101 aaggcgaaaa acggcccgct ggactgcgta cagaaaaatt accacgttgc cgaaagcact
    11161 ccagacagca aaccggcgat ggtagcggaa gattacgcca accgcctgcg taagaacctc
    11221 aaaaaattcg agaagtgggc tcgccaggaa ggaattgaat gttaccgcct gtatgacgcc
    11281 gatctgccgg aatataacgt tgccgttgac cgttatgccg actgggtggt ggtgcaggag
    11341 tatgcaccgc caaaaactat tgatgcgcac aaagcgcgtc agcgtctgtt cgatattatc
    11401 gctgcaacca tttcggtact ggggattgcg ccaaacaaac tggtgctgaa aacccgtgaa
    11461 cgccagaagg gcaaaaacca gtaccagaaa ctgggcgaga agggcgagtt tcttgaagtt
    11521 accgaatata acgctcactt gtgggtgaac ctgacggatt atctcgatac tggtctgttc
    11581 ctcgatcacc gtatcgcccg ccgtatgctc ggtcagatga gcaaaggcaa agatttcctc
    11641 aacctgtttt cttataccgg cagcgccacc gtgcatgcgg gattaggcgg tgcacgcagc
    11701 accaccaccg tggatatgtc gcgtacttat ctggaatggg cagaacgcaa cctgcgtctg
    11761 aatggcctga ccgggcgtgc gcatcgcctg attcaggccg attgcctggc gtggctgcgt
    11821 gaggcaaatg aacagttcga tctgatcttt atcgatccgc caaccttctc taactcaaaa
    11881 cgaatggaag atgcgtttga tgttcagcgc gatcatctgg cgctgatgaa agatttgaaa
    11941 cgtctgctgc gtgcaggtgg gacgatcatg ttctcgaaca acaaacgtgg cttccgtatg
    12001 gatctcgacg gcctggcaaa actgggactg aaagcacaag aaattacgca aaaaacgctc
    12061 tcccaggatt tcgcccgtaa ccgccagatc cacaactgct ggctgattac cgcagcctga
    12121 aaggaatagt aatgtcatta atcagtatgc atggcgcatg gctgtcgttc agcgacgcgc
    12181 cgcttctcga taacgcagaa ctgcatatcg aagataacga acgtgtttgt ctggtgggcc
    12241 gcaacggcgc aggcaaatcg acgttaatga aaatcctcaa ccgtgaacaa gggctggatg
    12301 acggtcgcat tatttacgag caggatttga ttgtagcgcg tctgcaacag gatccgccgc
    12361 gtaacgttga gggtagtgtt tatgatttcg ttgccgaagg cattgaagaa caagcggaat
    12421 acctgaaacg ctatcacgat atttcgcgcc tggtgatgaa cgacccgagc gagaaaaatc
    12481 tcaacgaact ggcaaaggtt caggaacagc tggatcacca caacctgtgg cagctggaaa
    12541 accgcatcaa cgaagtgctg gcgcaactgg ggttagatcc taacgttgcg ctgtcgtcgc
    12601 tttccggcgg ctggttgcgt aaagcggcat taggacgcgc gctggtgagc aatccgcgcg
    12661 tattgctgct ggacgaaccg actaaccatc tggatatcga aaccatcgac tggctggagg
    12721 ggttccttaa aaccttcaat gggacgatta ttttcatctc ccacgaccgt tcgtttatcc
    12781 gcaatatggc gacgcgcatt gttgatctcg atcgcggcaa gctggtgacc tatccgggca
    12841 attacgatca gtacctgctg gaaaaagaag aagccctgcg cgtggaagaa ctacaaaatg
    12901 ccgagttcga tcgcaaactg gcgcaggaag aagtgtggat tcgccagggg atcaaagcac
    12961 gccgtacccg taatgaaggc cgcgtacgcg ccctgaaagc gatgcgtcgc gagcgtggcg
    13021 aacgtcgcga agtgatgggt accgcaaaga tgcaggtgga agaggccagc cgctccggta
    13081 agatcgtttt cgaaatggaa gacgtttgct accaggttga cggaaagcaa ctggtgaaag
    13141 atttttctgc ccaggttcta cgtggcgaca aaattgccct gattgggccg aatggctgcg
    13201 gcaaaaccac gctgctgaaa ctgatgctcg gtcagcttca agcggacagc gggcgtattc
    13261 acgttggcac caaactggaa gtggcttatt tcgatcagca ccgcgcggaa cttgatcccg
    13321 ataaaacggt gatggataac cttgccgaag gtaagcaaga ggtgatggtt aacggcaagc
    13381 cacgccacgt tttgggctat ttgcaggact tcctgttcca tccgaaacgg gcgatgacgc
    13441 cagtacgtgc gctttctggc ggtgagcgga accgcttgct gctggcgcgt ttgttcctca
    13501 aaccaagcaa cttattgatt cttgacgaac cgaccaacga tcttgatgtc gaaacgctgg
    13561 agctgctgga agaactgatc gacagctacc agggcacggt attgctggta agccacgatc
    13621 gtcagtttgt tgataacacc gttacagaat gctggatctt cgaaggcggc ggtaaaattg
    13681 gtcgttatgt cggcggttat catgacgccc gtggtcagca agagcagtat gtggcgctca
    13741 aacagcctgc ggtgaaaaaa aacgaagaag ccgccgcgcc aaaagcagaa actgtaaaac
    13801 gcagcagtag caaactaagc tataaattgc agcgcgaact ggagcagcta ccgcaattgc
    13861 tcgaagatct ggaggcgaag ctggaagccc tacagacgca ggtggcggat gcttccttct
    13921 tcagtcagcc gcatgagcag acgcaaaaag tgctcgctga tatggctgcc gcagaacagg
    13981 aactggagca agcctttgaa cgctgggagt atcttgaagc gttaaaaaat ggtggctgat
    14041 cgcaaaatag tcgatttaaa cagagacagc tgacaacggt agaattgcct gatgcactgg
    14101 gtttatcagg catgaaaacc aacactttgt tagtcatctg taaaatagcg catcattaag
    14161 gagtaccaat gtgcgaacat catcatgccg cgaagcacat cctgtgctcg cagtgtgaca
    14221 tgctggtagc gttaccgcgc cttgagcatg gtcagaaagc ggcatgtccc cggtgtggca
    14281 caacgttaac cgtggcgtgg gatgcccccc ggcagcgtcc taccgcctat gcgttggctg
    14341 cactgttcat gctgttgctg tccaacttgt ttccttttgt gaatatgaac gttgcaggag
    14401 ttaccagtga aattacatta ctggaaattc ccggcgtgct tttttctgag gactacgcca
    14461 gcctcggcac ctttttcctg ttgtttgtgc aactggttcc cgcgttttgt ctgataacca
    14521 ttctgttact ggtgaatcgc gcggaattac cggttcgttt aaaagagcaa ctggcacggg
    14581 tgctttttca actcaaaacc tggggaatgg cggagatttt cctcgcgggt gtgctggtca
    14641 gtttcgttaa actgatggct tacggcagca ttggcgtagg cagcagcttt ctcccctggt
    14701 gtttattttg tgtcctgcaa ctgcgtgctt ttcagtgcgt tgatcgtcgc tggttatggg
    14761 acgatatcgc cccgatgcca gaactgcgcc agccgctaaa accaggcgtc acggggatac
    14821 gtcaggggct gcgttcgtgc tcctgttgta cggcaatcct tcctgctgat gaacccgtgt
    14881 gcccgcgctg tggtactaaa gggtacgttc gacgtagaaa cagcctgcaa tggacactcg
    14941 cgctgcttgt tacgtccatc atgctgtatc ttccggctaa tattttgccc atcatggtga
    15001 cggatttatt aggctcgaag atgccatcga cgattctcgc tggggtcatc ctgttatgga
    15061 gcgaaggctc ttatcccgtc gctgcggtga tcttccttgc cagtattatg gtgccaacgt
    15121 taaagatgat cgccatcgcg tggctgtgtt gggatgccaa agggcatggc aagcgcgaca
    15181 gtgaaagaat gcatttgatt tatgaagttg ttgagtttgt aggccgctgg tcgatgattg
    15241 acgttttcgt tatcgcggtg ctctcggcgc tggtgcgtat gggaggttta atgagtattt
    15301 atccggcaat gggtgcatta atgtttgctt tagtcgtcat aatgacaatg ttttctgcta
    15361 tgacgtttga cccgcgtttg tcgtgggatc gtcaacctga atcagagcat gaggagtcct
    15421 gacagtatgg aatctaataa tggggaagcc aaaatccaga aagtgaagaa ctggtctccc
    15481 gtgtggatat ttcctatcgt cacggcgctc attggggcct gggttctttt ttatcattac
    15541 agccatcagg gaccggaagt gaccctgatc accgcgaatg cggaaggaat tgaaggtggc
    15601 aaaaccacca ttaaaagccg tagcgttgac gtcggcgtgg ttgaaagcgc cacactcgct
    15661 gatgatttga cacacgttga aatcaaagcg cggctgaatt ccggtatgga aaaattgctg
    15721 cataaagata ccgtcttttg ggtggtgaaa ccgcagattg gtcgagaagg gattagcggt
    15781 ctgggaaccc tgctgtctgg ggtttatatc gaactgcagc caggcgcgaa aggcagcaaa
    15841 atggataaat acgatttgct ggactcgcca ccgttggccc cgcctgatgc gaaaggtatc
    15901 cgtgtgattc tcgatagcaa aaaagccggg cagctctcgc caggagatcc ggtgctgttc
    15961 cgtggctatc gggtaggttc ggttgaaacc agcaccttcg atacgcaaaa acgcaatatc
    16021 agttatcaac tgttcatcaa tgcaccttat gaccgactgg tgaccagcaa tgttcgcttc
    16081 tggaaagata gtggcattgc ggttgatctg acgtcagcgg gaatgcgtgt ggagatgggc
    16141 tcattgacaa cgctgctgag tggcggtgtc agctttgatg tgccggaagg tctggattta
    16201 gggcagccag tggcaccgaa aacagctttc gttttgtatg atgatcagaa gagcattcag
    16261 gattcgttgt acaccgatca cattgattat ctgatgttct ttaaagattc ggtacgcggt
    16321 ctgcaaccgg gagctccggt agaattccgg ggtattcgcc tcggtaccgt aagcaaagtg
    16381 ccattctttg cgccgaatat gcgtcagaca tttaacgatg attaccgtat tccggtgctg
    16441 attcgtatcg agccagagcg gctgaaaatg cagcttggcg aaaatgcgga tgttgttgag
    16501 caccttggcg aattgttgaa acgtggttta cgcggatcgc tgaaaaccgg aaacctggtc
    16561 actggcgcac tgtatgttga tctcgatttc tatccaaata cgcctgcaat aaccggtatt
    16621 cgtgaattta atggttatca gattatcccg accgttagcg gcggcctggc gcaaatccag
    16681 caacgactga tggaagcgtt ggataagatc aacaaactgc cattgaatcc gatgattgaa
    16741 caggcaacca gtacgctttc tgaaagtcag cgcacaatga aaaacctgca aacgacgctg
    16801 gatagcatga acaagatcct cgccagccag tcgatgcagc agttgccgac ggatatgcag
    16861 tcaacgttgc gtgaattgaa tcgcagcatg cagggcttcc agcccggctc cgcagcctac
    16921 aacaagatgg tggcggatat gcagcgcctt gatcaggtgt tgcgagaact gcaaccggtg
    16981 ctgaaaacgc tcaatgagaa gagtaacgcg ctggtatttg aagcgaagga caaaaaagat
    17041 ccagagccga agagggcgaa acaatgaaaa agtggctagt gacgattgca gcactgtggc
    17101 tggccggatg cagctccggc gaaattaata aaaactatta ccagttacct gtggtgcaga
    17161 gcggtacaca aagtaccgcc agccagggca atcgcctgtt atgggtagag caggtcgctg
    17221 tacctgacta tctggcgggg aatggtgtgg tttatcaaac cagtgatgtg aagtatgtga
    17281 ttgccaacaa caacttgtgg gccagcccgt tggatcaaca gttgcgcaac accctggtag
    17341 ccaacctgag cacgcaactg cccggctggg tggttgcctc ccagcctctg ggaagcgccc
    17401 aggacacgct caatgttacc gtaacggagt ttaacggtcg ctatgatggc aaggtcattg
    17461 tcagtggtga gtggctgttg aaccaccagg gacaactgat caaacgtccg ttccgtctgg
    17521 aaggagtgca aactcaggat ggttacgatg agatggttaa agtgctggcc ggtgtctgga
    17581 gtcaggaagc cgcttctatt gcacaagaga taaagcgtct accttaatta taaagatttg
    17641 taaatataac cgtctccggt atgttgcctg aggcggtttt tttgtttcta acgtgcggaa
    17701 aaatttgttc ctcttcacat tttttgtaca accgacatgc ccgtgtagct cacaaatatg
    17761 acagtggcgt gaattttgcg cattgacggc agttatgatt cgcggtattg cttaactgtg
    17821 attgcacatt tagtaatcac tgttttcttt tccaccagaa accagtatga gggaaacgag
    17881 gcatgaagag acaaaaacga gatcgcctgg aacgggcaca tcaacgtggt tatcaggccg
    17941 gcatcgccgg acgctcaaaa gaaatgtgtc cctatcagac gctgaatcaa aggtcacaat
    18001 ggctgggagg ctggcgagaa gccatggcgg acagggtagt aatggcctga ttctgtctct
    18061 ttaaaaagaa acctccgcat tgcggaggtt tcgccttttg atactctgtc tgatgataat
    18121 cagaaggcag acgtatcctg gaacagaccg actttcaggt cgctggcggt atagatcaga
    18181 cgaccatcaa ccagcacttc gccatctgcc agacccataa tcagacgacg attaacaatg
    18241 cgtttaaagt gaatacggta ggtcactttt ttcgctgtcg gcagtacctg accagtgaat
    18301 ttcacttcgc caacgcccag cgcgcggcct ttaccttcgc cgcccagcca gccgaggtag
    18361 aaccctacca gctgccacat tgcgtccagg cccaggcatc ccggcataac cggatcgcca
    18421 ataaagtggc atccgaagaa ccacagatcc ggattgatat ccagttctgc ttcaacatac
    18481 cctttgttga agttaccacc cgtttcggtc attttgacca cacggtccat catcagcatg
    18541 ttcggtgctg gcaattgcgg gcctttagcg ccaaacagtt caccgcgacc agaggcaaga
    18601 aggtcttctt ttgtatagga ttcgcgttta tctaccatgt tctctgtaag ccttatttta
    18661 ttgaagcacg caggatagct aacacgtgta cgctgaacaa gtccgatcag ttcggaataa
    18721 accagttcag ccaacgtaat ggccatggaa aacggtgacg tccttcctgt tgcgatgctt
    18781 gcgcgatacg ttcctggatg gtttgcatca gcgtcgtttg gccttcgcca tcccacacca
    18841 gatttaataa taacggcagt gcgtcagtca catcgtctac tgcccagatg gtgaatttgc
    18901 cttcttctac cgctttcacc agttcactgt gaagacttaa atggcgaacg ttagcagtgg
    18961 ggataatgac accttgttta cccgttaact cacgttgctg gcaaatagca aagaagcctt
    19021 cgattttctc atttaaacca ccgaccggct gggcgcgacc gaactgatcg actgaacctg
    19081 tgatagcgat actctgattc accggcacat cggcgagggc gcttatcaga gcgcagagtt
    19141 cagccatcga ggcactatcg ccatccactt cactgtatga ctgctcaaat gtcagcgatg
    19201 ctgagaaggg gatctgttgc tcaagctgta gttccgacat caggaacgcc tgcatgatca
    19261 tcatcccttt cgcatggata ttgccgccaa gctccgcttt gcgttcgatg tcggtgaatt
    19321 caccatcgcc aatatgcaca acgcagctaa tgcgagaagg ttcgccaaaa gcgcgtggat
    19381 gacccggaaa ttcaatgacc gaaagggcgt taatttgccc gatgcgttcg ccttcggttt
    19441 caatcaggat ttgctcctga aggatctcat cctgcatgcg ttcagcgagg aaaccttcgc
    19501 gccattcacg ctgctgtagc attaagttta gctgctcgcc ggagaaggtg tcgccatcac
    19561 acagggaggc gacttcttta cactggcgga ggatccactg cgggctaagc ggaagtgttt
    19621 cttgttcacc ggtgtagcgt gctgcttcgc ggataagtat cggccaggca tccgctcccg
    19681 gtgcaggtaa gtgattatgt ctggcggtaa atgtcaccca gcgacaccac tggcttactg
    19741 actccgcatc gacaatctgg agagtatctt caaattcgct ataaatagcc tgctctgaaa
    19801 gctctggctc catctcctgg aaatcagcca atgattcgcg ttcgcctacc agaatgactt
    19861 tcagcttcaa tggcatcgaa ggcacagaga cggggagagg gcgcgactca tcaaacgcaa
    19921 cccagtcaaa acgctcgcgg ttaacgatat ttttcagccg catccacagc agaggttgcg
    19981 ccagcagtgt acgcaaagag atgatgagaa taccgccatt tgcctgatgc accagaccag
    20041 gctgcagggt aatgtcgcca ttaaactggc gcaggcagcc aaagagttgc tccgcttcta
    20101 cccagtcggc agcgacaact tgcgttaaag tcgcaaaatt atcatctgca ctcactgcgt
    20161 tacgtaagcg gatggtgtgg ccggaaacct catagtgacc gcccaccagt tggcctgcat
    20221 cactttgtag cgcacgtgcc gcatcggcaa taagattcag atactcagac tcttccgggg
    20281 ccttcgccag cataaaagag gaggatgctc gcgtatgcag caactgctcc agcgcaaatt
    20341 gcagtcgcgg ttgagtatca ctgaataaag gatcgttttc gtcaatcaaa tgtggctgag
    20401 caaatatttc ctgatagcta tcggtatcag gaaccaggtc acgccatgca agtttcgtaa
    20461 tggtcaaagt tgatgttttt tagtctgttg tcaaagccgc gattataccg taaccggcac
    20521 tacagcacac gtagaaagca ccgacaatac tcctggcatg ggcgttaaag ctcacaggat
    20581 ggagattctt ttcttcactg gcctaaaaag ctgatattct gtaaagagtt acacggtaac
    20641 attgagatcg ctatgaaata tcaacaactt gaaaatcttg aaagcggttg gaaatggaag
    20701 tatctggtga agaagcatcg cgaaggggag ttaattaccc gttacataga agccagtgcc
    20761 gcccaggaag ccgtcgatgt gttgctctcg ctggaaaatg aacccgtgct ggtaaatggc
    20821 tggattgaca agcatatgaa tccggaactg gtcaatcgca tgaagcaaac cattcgggcc
    20881 agacggaagc gccattttaa tgcggaacac cagcatacgc gcaaaaaatc gatcgatctg
    20941 gaatttattg tctggcaacg tctggctggt cttgcgcagc gtcgcggtaa aacactttct
    21001 gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag agaaatacgc gaataaaatg
    21061 tcttctttga agcaggatct gcaggcattg ctgggtaagg aataactgat gaaactcagt
    21121 tcaatttact aaaggcgaaa aaaaaccccg cagctgcggg gttttttacc agacgagaac
    21181 ttaagcctgc ggctgagtta caacgtcttt gataccttta acttcgatct caacgcgacg
    21241 atccggagcc aggcagtcga tcagtgcagc acgctgtttc acgttgtcac aggtgttgcc
    21301 agtaaccggg ttggattcgc ccataccacg tgcggagatc ttgtctgccg ggataccttt
    21361 ggagatcagg taatcaacaa cagactgagc acggcgctcg gacagaccct ggttgtaagc
    21421 gtcagaaccg atgcggtcgg tgtaacccag aacaactacg gaaccgtctt tcggatccag
    21481 gttgctcagc tggctgtaca gctgatccag agcagcctga ccttccggtt tcagggttgc
    21541 tttgttgaag ttgaacagaa cgtcagactt cagagtgaag tgcttggtct gtacttccgg
    21601 tgccggagct ggagccggag caactaccgg agctgcttcg ccctgaccga aacggtagga
    21661 aacacccagg ctcagcaggc cgttgtccgg acgagtgccg atggtgtgtg cgtcaccgat
    21721 gttgttggtc cactggtatt ccagacgggt agcgatttca ggagtgatgg cccattcaac
    21781 accacctgcg aataccggag aaacgccggt atcgtggttt ttctcagatt cacctgtcac
    21841 attgttgtga gctttggtgt ctgcacgcca aaccatacca cccagacgag tgtacacgtc
    21901 caggtcgtca gtgattgggt aacccagttt agcggtcaac tgaacgccct gagctttgta
    21961 tgcaccgttt tcaacgctgc ctttgtacgg catacgacct aaccagtcgt aacccatttc
    22021 aaagccaaca tacgggttaa cctggtaacc accaaaagca ccagcgccca gttggttttc
    22081 atgggtcggg ccattgttgt cgatgaaacc agtgtcatgg tactgggacc agcccagttt
    22141 agcaccagtg taccaggtgt tatctttcgg agcggcctgc gctacggtag cgaaaccagc
    22201 cagtgccact gcaatcgcga tagctgtctt tttcattttt tgcgcctcgt tatcatccaa
    22261 aatacgccat gaatatctcc aacgagataa cacggttaaa tccttcaccg ggggatctgc
    22321 tcaatattaa ctctaccgat atcttcggct tatgccgagc acccctggcg atgtaaagtc
    22381 tacaacgtag ttgaaaactt acaagtgtga actccgtcag gcatatgaaa aaaaagtctt
    22441 gtataaggta tgtttaatct tttttgtcag cgacaattta cagaagaaaa tcgcggaaac
    22501 cgcttcagac aagcctccgc aaggaaaatt aatcacgact gaaagcattg gctggacgac
    22561 aaaaaaagtt ccaggattaa tcctaaattt acttaatgat acaaattaga gtgaattttt
    22621 agcccggaaa gttgtctcgt tgcgtgagag gatgcgctta ccggacgcat aataaacccc
    22681 atagcgttac cttcatttgc cgcatcaaca agttcagcat gctcttcttc agtcaaatca
    22741 tctgccaacc aaccgatcac cacactgtaa ttgcccgtgc gtaaagcgcg aaccattgac
    22801 tccacagtgt ggcaaggggg gagctggcta atctgcatta ctttcgttaa gggtagccca
    22861 gatgcctgaa cccattcccg actcagtttt tgttgcggtg ttaaccagag ttgccagcgc
    22921 gattgctgac cgagttgctg taacaatggc aacagtagaa gttgcgtcat catgggctga
    22981 tcttcgcgat agacaacttc actgataagc ccggctgtag tgttttccgt agagacacgc
    23041 gcaattttac ttgctgcgga tgagaacgac gaagaacgat gtgcatagcc tgaagtgtac
    23101 ataatcaatc cagcccctgt gagttactgt atggatgtac agtacatcca gtgacgacaa
    23161 agatcaaccc tattttcgga aagagcctcg caaattttgt cgttggtgac gggaaaacat
    23221 aaattaatct tgcccctaaa gaataagttg cctattttcg tatttaacgg atccgttaat
    23281 gtgaaccatt cttttatgtt atgattttaa aaggaatttt atgaaaagcc tctcctataa
    23341 gcggatctat aaatcacaag aatacctggc aacgttgggc acaattgaat accgatcatt
    23401 gtttggtagt tacagcctga ccgttgacga cacggtgttt gcgatggttt ctgatggtga
    23461 gttatatctt cgggcttgtg agcaaagtgc acagtactgt gtaaaacatc cgcctgtctg
    23521 gctgacatat aaaaagtgtg gccgatccgt taccctcaat tactatcggg ttgatgaaag
    23581 tctatggcga aatcaactga agctggtgcg tctgtcgaaa tattctctcg atgcagcgct
    23641 gaaagagaaa agcacgcgca atacccggga aagactgaaa gatttgccca atatgtcttt
    23701 tcatctggaa gcgattctcg gggaggtggg gattaaggat gtacgggcgt tacgtatact
    23761 tggggcaaaa atgtgttggt tgcgactgcg gcagcaaaac agtctggtga cagaaaagat
    23821 tctgtttatg cttgaaggtg ccattatcgg cattcatgaa gctgcgctcc cggtggcacg
    23881 ccgccaggag cttgcagaat gggctgactc tcttacgccg aaacaggagt ttcctgcgga
    23941 acttgagtaa tctggcgttg cagacgacca atctcaggca gtaatgcaat caataaccca
    24001 acttgttgta cgaccagagg ttctttgctg tctgcccgtg gttcaagttg ctgcatccgc
    24061 tgtttcaggc cagctaatgc ctgattgacg cgttcttcat cagcaggttg atgatgtaac
    24121 gcgtcatcaa catagcaaac tgcgtcatca agaaatgcca gaatttcagg attagttaac
    24181 tgctcccggt gagcaccgag ggctgagata tagctggtaa acgtatggtt aaggcacagc
    24241 aaccgaaacg cggcttcgcg aatttgcggg gtaacgttcg gttcgctgga catatttgat
    24301 accaccgacg ccagttcagc atcacggttg tgtgcatcgc ggcgggcaat acgatacgcc
    24361 agacggttat cacgcccctg atggtattgc tccagtatgg catcgagata gcgacagttg
    24421 gcctctgtgg cgcgttcgag catgcgcggc agattgcgaa actgccagtc aggccagatg
    24481 tagctcactg ccgcccacgc aatggcacaa ccaatcagcg tatcgattac gcgaggtaac
    24541 gctacttcaa aaccttcacc cagtaagtta aaacacagta gcaccaaaag tgtgatgaac
    24601 atcgttgcat gagcgtattg cacgttacgg aaggcaaaaa agagcacgcc ggtaataacc
    24661 agcagcacca gctgcccttc cagtgatggc acaaaccaca gcacaggaat gccaatggcg
    24721 atacctacca gcgtaccaat aatccttaac ttcaggcggt ggcgcgtggc gttatagttt
    24781 ggctggcaga caaacaaact tgtcagcaag atccaatacc cgtgatgcat tccggttatt
    24841 tgaatgatgg cgtagccgaa gcacaacacc agcgacattc ttaccgcatg acggaagagg
    24901 gcggattccg gcgtgaagtg acggctaaga cgcagccaga tatcactcaa tccgtgcggg
    24961 ctgtcatcag cgagctcatt ttcgtcatta ttatggggca gtgcctgggc ctgttctgat
    25021 tcaattgttg ccagttgggc atcaatggcg cgtaaattgt tcagcaaaaa tcccagtgtt
    25081 ttgagtaaat cggcaggcgc gccgttatcg cgcatccgct ccagggcagc atcaatatgc
    25141 gtaaaagcgc gctcaaaatg cggatcatgt tgataaggct gacgcagcaa aatacagcgt
    25201 gacagttgct ggcacgcctg gccctgcatc gacatcagcc gctgaaaacg gaacagcacg
    25261 tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa tatgagaaga gctggcacgc
    25321 tcgtgaatat cctgtgcgac aaagtaataa tgcagcgtgc gacgcgttcc ccgttgacca
    25381 cgatcgccac gtaagcgggt cagcagcgag agtttcgtct gattcaatgt cgccatcagc
    25441 tgaccgttgg cgagagccaa atcgtacagc ggtgcctggc tttcatcttc aatatcagga
    25501 tcaaacatgc gcgacttgag ctcaagataa cgcgccagtt gttcatagca acgcgccagg
    25561 ttgtcctgca gcgggcggac cgggaacagc agatgaccaa taagtgtcag gacgttgtac
    25621 cagacggcac cggccagcag atacatcggc tgctgatacc agtgctcata cagtgatgtt
    25681 cccaacatag tgtaaatagc gatcagcaat gcaccgaagg caattgttgc atagcgttga
    25741 cccagaccgc cgagcaaaat gaagccgctg gtagagagcg ttaagccaat cgcaaatagc
    25801 cagggccagg gaaacagcaa ttctactgag gccgaggcga taaaaaagca gaacagcgta
    25861 atgatgaggt tacgcaaacg tcccgccagt cggtcatcga gatcggtcag cgccgctgcc
    25921 accatcccca gcgtcagcgg aatcgtcagt tttacatcac ccagccacca cggaaacgct
    25981 gtggttccac aaagcgcaat aaaaatacgc gcgtaataca gccaggcgct gttccaggta
    26041 tagcgtttga gcaaaggact tagcatatag gccatattag tggattattc aaaacgacga
    26101 cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg ataccacgcg acgaccaacc
    26161 ggccatagtg caatagcggc aattttaaag ttcgcaatac cgacaggaat gccaatgatg
    26221 gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc ataaccacca gccaaagaaa
    26281 atcagccaga aaatattcaa taccgtaccg ccagtattca gcagcacatt tttgccagcc
    26341 gggttcagtt catcgacatg aatagcttca ttgccataag gcaccagaga cagtttagtg
    26401 atctcccagc aggatcgtgt cagcggtaag gtaaaaatca gcacaatact gaccagagtc
    26461 gccaacagcc agcccagagt ggtggcaaat ccgccaagca caaagtttag aatgttcaaa
    26521 acggtacgca taaaacctcg ctttactgtg gttttcagta atgggcggca attgtaacgt
    26581 ttttttgggc tggagcacgt tttctctgac ggttacactg ataagaaata atttcgtgtg
    26641 gatctacaga gtcatggaac tgaaagcgac aacgcttgga aaacgtctgg cacagcaccc
    26701 ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc tccggcgatc gccacgaata
    26761 ccttattcct ttcaatcaat tactggcgat tcactgtaag cgcggtctgg tatggggcga
    26821 gctggaattt gtactgccgg acgaaaaagt ggtgcgtctg cacggtaccg aatggggcga
    26881 gacgcagcgt ttttaccatc atcttgatgc tcactggcgg cggtggagtg gcgagatgag
    26941 cgaaattgcg tctggtgttt tacgccagca actggatttg attgccacgc gcactggaga
    27001 aaataaatgg ctgacgcgtg agcaaacctc tggcgttcag caacaaatcc gccaggcttt
    27061 gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc gataactgcc gtgaggcgtg
    27121 gcgtaaatgt caggcctggt tgaaagatat tgaaagcgct cggttgcagc ataaccaggc
    27181 gtataccgaa gccatgctta ccgagtatgc ggattttttc cgccaggtcg agtcttcacc
    27241 gctgaatccg gcgcaggccc gggcagtcgt taatggcgag cattctctgt tagtgctggc
    27301 aggtgcagga agcggaaaaa cgtcggtgct ggtggcccgt gcaggctggt tgctggcgcg
    27361 tggtgaagcg tcccctgagc aaattttatt gctggcgttt ggtcgcaaag ccgctgaaga
    27421 gatggacgag cgtattcgcg aacggctaca taccgaagac attaccgcac gcacgtttca
    27481 tgcgctggcg ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag tcagcaaact
    27541 ggaaaatgat accgctgccc gtcatgaact ctttattgct gagtggcgca agcaatgcag
    27601 cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg acggaagaaa tgcagtggtc
    27661 agtgccagaa ggtaacttct gggatgatga aaaattacag cgtcgtcttg cctcacgcct
    27721 cgatcgttgg gtaagtctga tgcggatgca cggtggtgca caggcagaaa tgattgccag
    27781 tgcacccgaa gagattcgcg atctgttcag taaacgtatc aagttgatgg ccccgttatt
    27841 aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg gccttattca
    27901 tcaggcgatt gtgattctgg agaaaggtcg ctttatcagc ccgtggaagc atattctggt
    27961 tgatgaattt caggatatct cgccgcagcg ggcagcgttg ttagcggcat tacgcaagca
    28021 aaacagtcag acgacgttgt tcgccgttgg tgatgactgg caggcgattt accgattcag
    28081 cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag gcgaacgctg
    28141 tgatttagac acgacttacc gttttaacag tcgtatcggt gaggtggcaa accggtttat
    28201 tcagcagaac ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg gagacaaaaa
    28261 agccgtcacg ttattggatg agagtcaact tgacgctttg ctggataagc tctctggtta
    28321 tgccaaaccg gaagagcgca ttctgatcct ggcgcgttac catcacatga ggcctgccag
    28381 cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc gactttatga ccattcatgc
    28441 cagcaaaggg caacaggcgg attacgtcat catcgttggc ttgcaggagg gaagtgatgg
    28501 ttttccggct gcggcgcggg agtcgattat ggaagaggcg ctactgccac cggttgagga
    28561 tttcccggac gctgaagaac ggcggttaat gtacgtggcg ctgacccggg cacgccatcg
    28621 ggtatgggca ctgtttaaca aagagaatcc ctctcccttt gtggaaatac tgaaaaatct
    28681 ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc tgtgcaatat
    28741 tacttcagac ggtccgcgag ataacgctga taatcgggga tcagaatatc gaccgcgtcg
    28801 ttgaaatgcg gcgactggat aatgaagtct gccgttgcca cgttggtggc gaccggaatg
    28861 ttccataccg tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg cacggcattt
    28921 agtggatccc agaagaaaat caatacatca attttccctt ctgagatcaa tgcgccaacc
    28981 tgctggtcac cccccattgg gccactcaac atcgcgttga cgttcatgcc ggttgcgcgg
    29041 gaaatcaagt taccggtagt gcctgttgca tacagtacgt gttgttccag taacggttga
    29101 tgtcgttcca cccagctcat cagcatttgt ttgcagtgat cgtgtgctac cagcgcaata
    29161 tgtttccgcg caggtaaagt gcgagtcgtc agttccataa tgtacatccg tagttaactt
    29221 tcctacagat tactgtaagc acttatcgct gcaagataaa gaccgaaaaa gcctgcgcac
    29281 aggcacaaaa atctcaggaa gatggttgtt tttccgccca ttgcaggaaa gtatttcgcg
    29341 tttgtgggtc agccagttta aaccaatact tcagccgttg ttctgtgagc acctgagact
    29401 gcggtggaat agcatccagc tcggaaacac ccgaaagcag cgtactgtca tctgccatca
    29461 tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt tcaacctcga
    29521 cctcataatc aatcgttttt gccgttgagg taatggcgag aatatccagc tttaccggaa
    29581 tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc ggcatcaaaa tggttagctt
    29641 cccgctcatt ctccaggcga ggcaggcgaa aattgacctg gttgatgagc tgggtattaa
    29701 aactgaccac cagcggtggg gagatataca gccgttcttc actattggaa agatgaatcg
    29761 tcttctcaac gcgaaacact aactgatgcg gtccattatc cagttcaatg ctgtccgcac
    29821 cgcgcagcag agagctggaa accttcttgc cgtcgagtac cagaagatcg acatcggttg
    29881 aaagccgcag cgtggtggca aaaacggata ccggcaaaca taatgcaatc aaggtggtca
    29941 cgatgccggt tttcataggt agctcctgtc aaaagaccgc ccgaataata atgtgtcaaa
    30001 atttttcttt aatcatactt actaacatat agacatattt cccgcttttg ctctcattca
    30061 ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta cactttgtga aacagtctgg
    30121 aggaaataat gaaagaaacc gatattgcag gcattttaac gtccactcat accattgcgc
    30181 tggtgggggc aagcgacaaa cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg
    30241 accaggggta tcacgtcatt ccggtttcgc caaaagttgc cggcaaaacg ctgctaggcc
    30301 agcaggggta tggcacgctg gcagacgttc cggaaaaggt cgatatggtc gatgttttcc
    30361 gtaattcaga agcggcgtgg ggcgtggcac aggaagctat tgccattggc gcgaaaaccc
    30421 tctggatgca actgggggtg attaatgagc aagcggcagt actggcacgg gatgccgggt
    30481 taaatgtagt gatggatcgc tgcccggcta ttgagatccc tcgcctgggc ctggccaaat
    30541 aaaaaatccc cggaaggcaa aaaccttccg gggatttgtt cagggattag ttacgcagac
    30601 gcggggcctg gagttgtttg cggatggtct gcgccagctc atccatggat ggctgttcag
    30661 gatgttcatc ctgcaattcg ctacttagct gggcttcagc caggtaggta tgaaccggta
    30721 ggccgttatc gtcctccatc accacgtgat accacggagc ggcgcgaagc tcgtcattca
    30781 ccgccagctc atcaggcgac ggttcagaga gcgaataaac cgggtcgata tccacgacca
    30841 ctccgagata acctaacagg gaatggcgga cctgctggcc gataccgaat ttgctggcaa
    30901 tcatagtcac ctcccgggaa atctttacct ttgatataag ggtaaaattc cacatttcaa
    30961 gttacatgac gcgacaggca aaccctttca gatatagccc ttccggatag gtagcgatca
    31021 ccggatgatc ggctgcctga cggaactgct ctataaattg tacatcacgg ccggcatcaa
    31081 ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt catcagaccg gaacaggaga
    31141 aagtcaggag aataccgcct tcattcagca actgaatcgc cagcatgttg atgtctttat
    31201 agccacgaca cgcgcccatc aactggcttt tattctcaac aaacttcggc gggtccatca
    31261 cgataacgtc aaatttttca ccgcgatcgc gataagtacg cagcaattta aagacatcat
    31321 cacggacaaa ctcagccttg ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa
    31381 tatccagggc ttcctgggag gtatcaacgc tgacaacctg gttgcaaccg cccatcagtg
    31441 ccgatacggc gaaaccaccg gtataggaga aacagttcag tacgcgttta ttttcaacgt
    31501 agcggcgggt agccaggcgg ctgtcacgct ggtccaggta gtagcccgtt ttgtgtccgt
    31561 gctgaatatc taccagcagc ttcatgccat gttcttcaat cggcagcagg gcaggcggca
    31621 actcgccggt gacgaggccc tgggtcagct ccatcccttc ttttttacgt accgcaacat
    31681 cgctgcgatc gtaaatcgca cattccgggt acagcgtttg cagggcgcta attaatgccg
    31741 cgcgctgata ctctgcccca gcactcagca gttgcagcac cagaaaatta ccgaaacgat
    31801 cgatagtaat acccggcagg ccatcagatt ctccggcgat taaacgatag ctgtcgaggc
    31861 catctttttg cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg cgggaaaaaa
    31921 aagcaatgtc gatagactca gacgggtcaa acgtccagac gcgcgcccgg atttgcgaag
    31981 ctggcgaata agcgccgcgt gctaaccatt ttccctgatg atcaacaata tcgatggttt
    32041 caccgaggct ggctttacct tccatacggg caacggcccc ggaaaagacc cacggatggc
    32101 gacgaagtaa tgatttttcg cgccctttgg ctaacactaa acgtacactc ataatttact
    32161 tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga tttgcaacgc
    32221 gtccattaag gataaggaga gcagcaatgt cgaaagtctg cataattgcc tgggtttacg
    32281 ggcgagttca gggcgtagga tttcgctaca ccacacagta cgaagcgaaa agactgggct
    32341 taaccgggta cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag
    32401 aagggcaggt agaaaaatta atgcagtggt taaagagtgg cggcccgcgt tctgcgcgtg
    32461 tagaacgggt gctcagcgag ccgcatcatc cctcggggga attaaccgat tttcgtatcc
    32521 gttaaataca ttttaccggt tttggcaggc cagcaatttt ggtggcttgc ttagccggac
    32581 ctttcggaaa cagtcggtat aaatagcggc tgttaccttt ttcttcgcca aatttattcg
    32641 ccatcgcttt taccagcata cgaatcgccg gagaagtatt gaattccaga tagaaatcac
    32701 gcacaaaacg caccacttcc cagtgttctg gcgacagcga aatcccttcg ttctctgcaa
    32761 tcaccactgc cagcggctca ctccactggc tgctttcttt gagatagcct tcggtatccg
    32821 tttctatctc tttaccttcg aagatcagca taattattac taccttaatc agactgccgg
    32881 cagtgtaaca aaaaacaaag ccccgcataa agcgaggcta tgaaagtgtt agcgggtgag
    32941 attaatcgcg gctggcgaag cccagaatgc tcagcaggct gacgaagatg ttgtacagcg
    33001 aaacatacag gctaaccgtg gcacgaatat agttcgtttc accgccatga atgatgttgc
    33061 tggtttcaaa caaaatagcg ccagaggaga tcaaaatgaa gaccgcgctg atcgccagat
    33121 gcagagcagg cagttgcagg aagatattag caaccatacc aatcagcacc accacaatac
    33181 ccgccatcag cataccgccg aggaacgaca tatctttgcg ggtggtcagc acatatgcag
    33241 agcaacagaa gaacactaac gccgttccgc ccagtgccat agcgattacg tcacccattc
    33301 cggcagacag ataggtattc agaattggtc cgaggatata acccagaaaa ccggtaaagg
    33361 cgaatgcgga gataatcccg gtcggcttat tcgccgtttt ataggtcagg aacatcaaac
    33421 catacatacc caccagcgtc agaatcagac ccggagatgg cagcatcagc accgtactgg
    33481 cagttgcggt aatcgccgaa aaggccagcg tcaggctcag cagaaaatag gtattacgca
    33541 gcaccttatg ggtgctaagc agtgatgtac ggtcatgtga agaactaaca atacgatcca
    33601 ttgagtcact ctctatgaca gatgtaatta attaagcagc ataatgataa tgcgtaaggg
    33661 cacccagaag ttttacccat ctttacgcat ttgatctgga acaggtttaa cagcggatta
    33721 tcaggtcatt aagcaaatat aacgccctga gaatttcgac aggcaaaaga aaaaggggtt
    33781 agcatttagc taacccctta tcttatctgg cggaagcgca gagattcgaa ctctggaacc
    33841 ctttcgggtc gccggttttc aagaccgatg aaaaataaat aaaaatcaat gcaatactaa
    33901 aaacagctga aaaattaacc aaacttaaca cctagaaaaa tcaggctgtt agggagcttt
    33961 cagagttagt ataccaagtc ttttttgcca gaaaatttcc tgagttgcct acttgctgac
    34021 cacaagtggc aaatccagtg tcggcgtgat ttttgtttta cgatcataaa tcaacacctg
    34081 attttctgtt ttatgtccac tgaaaatctg tttgtcgcga ctgcttcctt cgtaatctga
    34141 tattcctttg gcttttatgt catggaaatt gcacccaaac gggacgccgg attttttctc
    34201 ggctgcgtgt ttagcctggt tccaccagtt gtttagcgtt ttagctatga ccttcccgcc
    34261 cttggttgtg ttgatcacat attcacatgt gcctgaagat acatttctgg ctaactggat
    34321 cgcggttcgt aatcgtggag accattcctt gatttgtttg gtgccggtct tgttttgctc
    34381 aatgtagatt cctttatcca taatatcctg ccatttcagc tcgagcacat caccgagtct
    34441 tgccgcacaa agataggata tctccattgc aatgcgtaac tgtggtattg cttccgcata
    34501 tatcgccgca tactgttcat ctgtgatgta aacagtgcgg gctttaagcg taaatttcct
    34561 gactcctttg catgggttat tcttcacata tccacgctca tatccccacc cgtatacacg
    34621 actgagactt gccagttcat ggtttgcctg ggtcttgctc tcaatccccc gtttatccat
    34681 gaaaattctt acctgctcaa tttttacatt atcagccagt acttttccga agactgccag
    34741 taacgccctc tgatgttgcc gataatcttt ttgggttctg ggggccagtt ctgtaaatgc
    34801 gggggaatcc ataaacatgt gccataattt agcgacggtc attatgttgt ggcgttttgc
    34861 tttttccagt tcataattct gccagacttt agctatgctg gtttcccgca ctcttcctag
    34921 ccctatagtt tttgtgcttc cttcgggttt ccatacgtaa ctgtaaccat tcgatctaac
    34981 acgtggcggt agtgcattat cttttttgtt ttttcttggt cttcccattg ttcagcgcct
    35041 caaaatcggg gtcagcagaa accagttcag atactttcgg tatcgcagtc agtccgcgcg
    35101 ggacatcact gcgaagaact attggttcgt ttttaggacc gggtacaaat gggataccgt
    35161 gcaaccttaa ctgttgttgc tgttttgtat accgttcgta tctcgtgatc tcttgaattt
    35221 ctgctggcga tagagttaat tcgtacatgt ggtcacgtgc cttacagcat gaccgccgcc
    35281 actatagctt gtgggcggcg atatgggttg aacattaaaa atcagcctga ttcgggaaca
    35341 gtttttgcca gatagctgaa acgtattttg cctggtaacg cgcatcgtgc agggcattat
    35401 ggcgttcacc ctcaaatgtg atgacacttc gagcatcgaa atccatcacc agccccaggg
    35461 caacaatcgt tcttacatca cgatcattgg tgtaacgcca cgggcagggg atctcctgcc
    35521 gttcatatga acggcgtaaa atcacgttgt cgaagttggc tccatttccc cagacctgaa
    35581 caaaaaactc accggagttt tcgtctataa attcccgtaa ttgtaaaagc gcatcatcaa
    35641 gcggtattcg atcagctaaa atggcggcac gggcttcgcc agagcgttga agccaccatt
    35701 cgatggtgct tatgtctaca gtgccgccac atgtacccat atcaattatt ttgctgaatt
    35761 caggcccctg ctctccggtt tgtggatcga aaaatacagc gcctattgag gctattggtg
    35821 cgtcaggatt tttgcccatt gtttcaaggt caatcatcag atggtgccac aacctgctgg
    35881 tggatgtgat ttcacgatga ccgttcacct taattaaggg atttgctgtc tcgcctgttt
    35941 cattattgcc attggcatgt tgatcgcctt cggtgcactc cttgttcggg agtccggcac
    36001 tttccatttc ctccggatct ttttcccggg cttcatcctg gctttcttcg ttgaatgtct
    36061 cctggtaggt tgcgtccccc atcactgcgc cacaatcagg gcagttacca ccaccagtct
    36121 gattgcatgc ggtacaaact ttttcctctt cctgttgcac tactggttca ggttgtttcg
    36181 tttctggctc gttttgtaac gcatttgggc tgttttgttc cgctttctga ccgttctgtt
    36241 ccgtttcttg ctggttctgg ttcacagaat cgcaggtttc aatcccctta acccatttcg
    36301 aatcattcgg gtcgctaatc ccttcaacaa actcaccacg agaagcggca agcaatttgt
    36361 cggcatctgg tcgactgatg tctgctgcct ccataatttt atttacttct tcagcagtaa
    36421 cttttacctg tgacttgtca tccagcggct gcgggtcctg atgatgttca actgtatccg
    36481 ctgccattgt ttcagccgtt gcctgttcat ctgccattgc gccagatggt tgcgggtttt
    36541 cttcatcatt tttttcttct tctgttttgc acttagcagc cagtccgccg ttaatttctt
    36601 ccaggatatc tttttccggt gtatgccggg cagccatgag cgtttcagct gtgggattct
    36661 cgtgatcagt ttctgtcagg ttggcattga tatacccctg aaggcgtccc gggtagtgat
    36721 aaaactcagg gtgggcgctc cggataagtg caaaaatagc tgcgcgggaa tagtccagga
    36781 taccaggggt tgcgcgaagt gctgcggacc attccctgaa cggactttct tttttcagga
    36841 cgatttcttt tgcgcgacga taaacgctca cgggtatttc ataaatatta aaatccatcg
    36901 gaagtgtggc tgctgcagcc tccacatcca gagtatcgag ggtgtgtact aagtcaggat
    36961 tacgatcggt tttatttccg ccaccggcat ttgcaccgga agccgtgcgg gtgatgcgag
    37021 aaacacgatt tcctttcatc cactcttttg tcagcagacc tcgatcggta tagtcagcgt
    37081 ccaggtatgc ttcgaaaaaa gcagtcatta accccagact ggaattaacg ggattaggga
    37141 aaactttgtc tgtgtcgcgt accagtttgt gaaggtcgcg aatctccagc gggtcgagca
    37201 gctttgtgtt gtgggaaata gccagggcag taacagccgg cagttcttca gctcgtgcga
    37261 tatgtaatgc ctgaagttct tcccgtgaaa cgtgcgttac cggtttttcg ctgccatgtt
    37321 gcgcaagcca gcgaatgggc agttcctgac cggaaatcgg caggagcata ttatcctcaa
    37381 tctcagccat gtcttcgcca ttgacgttgg tattgtcagt gctggctggt ttgtcctgaa
    37441 ctgaggggga aggcgctata aatatcattg tgatgccatc ttccccgcct ttttcatagc
    37501 ggttgcagaa ttcggtatca aacacgcctt ccggtggaag gtcattcaca acggggaaat
    37561 ggacgcggac aggttttttg aaatcatcag cgtcgtagcc tgcctcgtcc attgcaacaa
    37621 cgcaccgtga gatggcaaca gataattttt ttgctgtagt ccagtaaaaa cctcctttaa
    37681 tcccaaggcg ttttctgacc ttgtcatttt ttgcttcgca atatagcgaa aattcttctt
    37741 tatcagtgct catggataaa cctcataact attttaaggt tgtacgaacc cctgccattg
    37801 ctggcatgct taatcaacgg gtatggcgtt aatatggctg gtggattatc cagccggtat
    37861 ttcgttattc aggtacagcg atactttttt taacgggagg cattcgccag aaattttttg
    37921 ctcgtctctt gcacggaggc atgattcttt actggcataa attccggtaa tcacattctg
    37981 tggctcaccc gttataagaa aaaccgtcat caccagtgca aatgcagaag tcactgctgc
    38041 cctccgaaaa taccaagttt aagaagggca attctggaga gtatggaatt atcattgaga
    38101 agataaggct catattttct catcttaatg gcatcttcag taaactcccg gttactgagc
    38161 agaataccaa tatcaaaaca cccttcagac gtattaacgt ttggtaataa cgtttccatt
    38221 atcgcgtcct caacaatgaa tttttagtaa ttgctccaca gtcatatttt taattgcgct
    38281 ccggtttaca agagtccagc cttgtttctc cagataaaac tggaaggtat ccagggtgca
    38341 gaccattgca ccatcaggaa cggtttcagt gaatttgata ttgccgtgtt cgtcgagacg
    38401 gataaccagg gtacgtccgt ccccgtgaat cattttgtcg ggagacgggg cgttattctg
    38461 gcgcagttct ttctccatgc gatcgaactc agcgatgtag gcttctttaa atgcagcggc
    38521 ttttttgccg gtgaagccca tcaccaggaa aacgaagccg tttttggtga tttggtacat
    38581 tgggagtttg cgaccggttg agtcggtata ttcgctcgac acaaaattgt gctcagtgaa
    38641 ttttgctgaa cagtccagat tgcgaatttt atccaacact cgttcgtggc gtttaccaaa
    38701 gaactcagcg atcgcaatag acgtagtgac agcgcgtcca ttttcgatgg ttacgtcagg
    38761 gtgagaaagg gcagggatag tagccatgat ggcagcctcg atagtcagtt taaataactc
    38821 accaccaagg ctttccacga ccataagggt ggtgagacgt acaggggtgg aaataccggt
    38881 gactaccgaa accggccagc ctttcggctg ccctgcacgc cccaccataa tctgaatgtg
    38941 gctgtgcatt acgcataaaa aaaccgcctg agcgcggtta tgcgcagtag tcatcatcgg
    39001 gtttccacgc ccggcacccg ctttatgagg tgcaggtgca ctataattcc acccgttctg
    39061 gttttcaata gctacattca acattttctc ttacccttca tcactgaagt gaactttgtt
    39121 gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg ccggaataca
    39181 gaaggatgcc cgttacgccc cgtaaaagac cactttacgg ttttaactgt tccgcgtgcg
    39241 cttagccgca ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagaaagcta
    39301 ataaactgat gccgccaaag gtaccactgc cattgaaaca tggagatgtt gtggcggggt
    39361 tgtcactcag gcatatggtc aacctgacaa cccggtgtcc tcaatgaggg gaaaagaaat
    39421 aaaaaccccc agccatattt accgccgcgc catttcgcgg attgccacaa ccggaagcac
    39481 acggtcgaag aaatctaacg acaagccttc taaggaaaag acgccttcgc cgtgtgcttt
    39541 ctgttgtgcc ctgacttttc agggtaaatt aaccagtggc aacctgtttt tactggtgct
    39601 aatcagttag tgtttctggc taaccagcga cgcgcgccag cttcggtttt aaacttttta
    39661 cttttggtat acgtcatcgc ggtaaacgtg ccgtcctggt tagggaacac accgcatacc
    39721 agagattcgt tgttgccaag attgagcgta tccatgttga cctcatttcc ccttaacgcc
    39781 ggggtagcgg aacaaaaaac ctgctgcata gttaaacgta atccccgccg tcatgttcat
    39841 acgcctcagg ctggctactt aacccctgac cactgccggg taactcgaag tattgccctg
    39901 cgttctgtgg ggcggggtgg gtttatggat acaatttaca aattaaaatt taactggtca
    39961 acatgtttat tattaaattt gtaattgcga gctgttgcat cagtcccaaa atgggactac
    40021 tatacagtta tgaagattat ctcagttaaa acactcaggg atttttgggc ggagaatcct
    40081 gatgcagaac aaccgctaag ggcatgggtg gatgaggcgg caaaagctga ctggaaaagc
    40141 ccagcagaca ttaaggcaca ataccgaacg gccagtatat tgaaaaaccg gcgcgtggta
    40201 ttcaacataa aaggtaatca ttaccgttta attgttgcaa ttgcgtatca gcgaggatgg
    40261 gtatttgtta aattcatcgg aaaccacaag gaatacgatg ctattgatgc tgataccatc
    40321 gaactggagt aagcatgaac atcaaaccta ttcgtacaga gcaagattat gaagccgcgt
    40381 tgcgtgctgt tgaacccatg ttcgacaatg agcccgaaat tgacacgcct gagggggatt
    40441 tttttgaggt gatgtgtttg ctaatagagg aatatgaaaa aaaacattat cccattgagc
    40501 caccatcccc aattgaagct ataagatttc gcatggaaca gcaggggctg actgtgaaag
    40561 atttggaacc cgcaattggg aaaaaaaatc gggtttatga ggtgttgaat ggtaccagaa
    40621 gccttacgtt accaatgatt cgccgtcttc ataatcaatt tggtatcccc ctggaaagcc
    40681 tggttggatt ataaaatctg ctagtcattt gcctgatgct cgttccagaa aaggaatgca
    40741 tcaggcagtt ttgtttttct gccgcagtaa ctcttcaagt ttccgtttat agaaatcgcg
    40801 tttttgctcc atatcacgaa tgatctgctc tgcgtcgctt tgaggtaact catctaaaag
    40861 cgatatgatt tttcgttgtt gttctgtaag ttgcggttgg ttgtcattac tggatacagc
    40921 cattttatcg ccgagagttt cttcttccat aaagaaccaa tggacgggat gttgtgagag
    40981 ctctgctaat ttttccagtt tatccattct tggcatcacg cctttcaacc aaccttgcac
    41041 ggattggggt tttacaccaa gacgtcttcc cagctctgac tggtttatat tcaattcctg
    41101 caacacctgc tgaaggcgtt ttacaaagat catcaccacc cctcgtaaac tagttccgcg
    41161 atcctacaga aaaaattgat aagtggcatt acaaatagaa gttgaaattt aaatttaaat
    41221 ttgtaatcat cggtgtcatc gtaaagttcg gagggaaaca tgcaaaaaag tactcaagtg
    41281 aaaatcctgt caataatgag ccaatcagaa ttagggcgtc gtcttggtaa aacaccgcaa
    41341 accataagtg ggtggtttaa aaaacgagtg ccagcggagg aggttatacc agcatgtgag
    41401 gcgcttgact ggggagtaac tccgcatgaa ttgcgccctg ataaataccc taatccaacc
    41461 gatggtttac ctgttgagta tcaggctaac gaacaagcag cggcgggagt tgattcatga
    41521 aaatcaagca tgagcacatc cgcatggcga tgaatgcgtg gctgctttat ccgagggtag
    41581 ggcgcaaaaa aatcgctgat gatatagcga cagcatattt tgagcttgaa atgacttatc
    41641 caccaatgca tgacacctct acgacagagg gtattggatt gaatatacaa aatatttttc
    41701 gctggcttga aaaggatacg cctgatgctg ttgaaaaaat tcaggcacta attccggcta
    41761 tcctgactgt tcttccgcgt gaactgcgtt atcacctcag tatttttgac actgttgagc
    41821 gccgtgcatt actggcggcg caggaagcgt tgagcacggc aattgatgca catgatgatg
    41881 ctgtccaggc cgtttaccgg aaagcgtatt tcagcgacgg cgggtcatcc ggcgagtctg
    41941 ttgtggtgca ttgatattta tgccagcccc ctgctgattc tgttgattgg ggaatcacag
    42001 aatatcacca gaggatggtt cgtcacaaga tgaggcaatt atggccgcat taccatacat
    42061 gcaactgtac atagctgatt acctagctga caccatgcat ttgtccgcag aggagcacgg
    42121 tgcgtatttg ttgctgatgt tcaattactg gcaaacaggg aaaccaatac ctaaaaacag
    42181 gctggcaaaa atttcccgtc tgactaacga gcgatgggct gatgtggaac catccttgcg
    42241 ggagtttttt tgcgataacg gcgatgaatg ggtgcatctt cggattgagg aagatctggc
    42301 atcagtcagg gaaaaattaa ccaaaaaatc agccgccgga aaagcatctg ttcaggccag
    42361 aagaagcaga aaggaagcat atgttcaaac aaaacaagag agagatttaa caggcgttca
    42421 aacaaatgtt ggtgttgtgt ttgaacatga cgcaaacaca aaagcaacta ataaagatac
    42481 agatctaaaa gaattaaacc ccacacataa cgtgcgtgaa cgcgagagta ttccgaccag
    42541 tgagtcgcat ggtgcgccgt tgcagacagc cgaacctgaa tacctggacg gcctgagcga
    42601 accgatcggg aaattttcga tgaccactgt ctggcagccg tcgccggatt ttcgacaacg
    42661 ggcagcagtg tggggtatgg ctctgcctga gccggaattt acacctgctg agcttgccgc
    42721 attccgggat tactggatgg cggagaggaa ggttttcaca caggttcagt gggagcagaa
    42781 atttgcccgc cacgtgcagc acgtcagggc acaggtaaaa ccagtcagca aggggggaag
    42841 ccatgcagca tcaggtggca cggcatcacg ggcagttcag gaaatccggg cagcacgcga
    42901 acagtgggaa cgagacaacg gatttatcag caacggaaac ggcctggaag ctgtgggagc
    42961 ttatggggga ggtgtattcg aaccgctgga ctcagaagaa cggggccgca ccttcgaagc
    43021 tctggattgc ccagattggt gcgatgactg aacagcaaat ccggcaggtc tgccgtcagt
    43081 gcatggaccg ctgccgggcg ggtgaaacgt ggcccccgga cctggctgag tttgttgcac
    43141 tgatttcgga gagtggggca aatccatttg gtcttacggt ggatgcagtg atggaagagt
    43201 accggcgctg gcgcaatgaa tcctggcgat acgacgggag tgataaatac ccgtggccac
    43261 agcctgtgct gtaccacatc tgcctcgaaa tgcgtaccag agggattgag cgccagatga
    43321 cgcagggtga gttaaaacga cttgcggaac ggcaactgac gaaatgggca aagcatgttg
    43381 gtaacgggat gagtgttccg ccagtgcgac gacaactgga aggggcgaaa tacccgaaag
    43441 ggccaacgcc aattgaatgg ctgaaacagg aatacgaacg ccggaaggca gctggtttta
    43501 tttgaatctg agaagcgatt ttgtaggagg aaattttaat ggaaaccgtt tttgacgcac
    43561 tgaaagcaat gggaaaagcc agctctgttg gactggctga gcggcttgat atcagtcgtg
    43621 aagaggttct caacgaactg tgggaactca aaagaaatgg cgttgttgat aaagcgggtc
    43681 acatctggtt tctggctggc gaaggtgaat ccggggtaac cgaagagcag ccagaacagt
    43741 ctgaagtacc ggatgtgctg accggggagg tcgaacaaaa agttaccgca gacatgatga
    43801 ttgagtttat cggtcaggag ggggctaaaa cgtgtgagga actggcggat aagttcggtg
    43861 ttagcattcg caaggttgct tccacgttgg cggtggtaac cgcaacggga cgcctggcac
    43921 gcgtaaatca gaacggtaaa tttcgttact gcatgccggg cgataattta ccagcagagc
    43981 cgaaagccgt gccggtaacg gaaaatgatg gtaaggcctt tcctcagcca gcaggtgttg
    44041 cgttaccagt acaggaatct gcaacacagg aagatattaa aacagaaaca gtggcggaca
    44101 ttgtgcagtc gttgccatcg tttaccgaaa cgcaagcaga tgagctgatt tttctgtccc
    44161 tgtgcagggc aaacctggcg ctgcgcaggg cgaaaagtga tgttcagaag tgggagcgag
    44221 tctgtgccgc gctgcgggaa ctgaacaaac acagggatat tctccgggat attaccgcca
    44281 ccagagagct gctgcggtga gtggctggaa gaagtggcgc tgggcggaaa ttatgatact
    44341 ccggcagtgt gcgggaacga tgacagtcga aagcatcggc agtctgattg gccgtagtgg
    44401 ggctgccgtc aggacgaaag cgcgggaact gggtatctgc atgatgttac ggggtgatta
    44461 tcaccagtca gccaaatgtt cacagcgtga tattgagctg gcgcggcaac tgcatcagcg
    44521 tggcgtaccc cgacgggaaa ttgccgaaaa gtttgggatg aagttgggcg cagtgaataa
    44581 ctacgtttat ttcgacagga gggttcaggc gtgagggtga gaatttatat cgccggtccg
    44641 atgacgggat atgaaaattt caaccgtgag gcgtttcaca gggcggaaga agcactgaaa
    44701 cgggaagggc ataccgtttt aaacccggca gtacttccgg acggactgac acagccacac
    44761 tacatggata tttgcatggc gatgatacgc tgcgtggatg cgatttacat gctgaaaggc
    44821 tggcagcggt cggcaggcgc taaggcagaa ctggcactgg cggagaaact gggacatgcg
    44881 gttattttcc aggaggcaac cagtgagaaa aattaattat cagacgctac gtgagatagc
    44941 aaaactggca acccagggcg aatgggtcgc attcatttcg ccgggcactg atacatatgc
    45001 ggtgcatacg ccgggggatg aacgttgcgg agacattatc aaatggcctg gctttgatga
    45061 ccagaaaaat gcagagaaca acgcagaatt tattgcagct ttcaaccctg aagtagtaca
    45121 ggcactgctg gatgaacggg aagcccaaag caaacgtatt gcagagctgg aagctaatct
    45181 tgtggcgctg gcggcggaga atgcacttgc tcgtaaagca gttcaggcat tctgcgatgt
    45241 tgttggcgac aacaccgagg ttatctgcga ggaggttggg agagatggcg ttctggttat
    45301 tttggaggcc atgaaggcaa caggaaatat gccagccacc gatgctttcc tgtctgaagt
    45361 gcgggcgctg ggggtggatg ctgctataga agctgcaaaa aatctggtgg cccaagaata
    45421 tgagtataag gatttcaaag cggcgcagag tgattgctgt atgcaccctg gttcagacct
    45481 gatagggaag gttgaaatga ctgagtggtt agttgacttt gctgcccagc ttcgcaaagg
    45541 agttcagtca tgagagcttc aattagttat gtcgacgatt gccacctaag cgtgcgtgtt
    45601 gatgaaattg tgtcatccgt gccaactttt ccgacaaaaa atgcagccgt taatgcaggc
    45661 gcgccattcg gatggaggac ggcagttcgt attgagcgtc gattcgaaaa cgtttgggtg
    45721 gtaggtaaga aatgcttcca aagcgatcgc tctgcggggc ttaattttga agcatatcga
    45781 ttcccgttcc tccgatggga gaaagagggc gggattacaa aatgcccgat tctttccgtg
    45841 cgcagattca agcaggaggc aaccagtgag caagattgac tatcaggcat tgcgtgaggc
    45901 agcacaaaac tatcggtcga tgctggcgtg gtatcaggag aaaccagaca gtccaaacgc
    45961 tgagcaggat tgtgatgcag ctttggctgc gtttaagcgt gaaatccgtc atcgtgaagt
    46021 gggtattatc gctgatttgc tggatgaact ggaggaagca aaacagcgta tcgacgagca
    46081 ggagtcccgc atagtgaagt taccagaacc attcaagctg gctaaatcat caagtggatt
    46141 aacgtactac tacgctgatg aggtcaatgc cgcactgact gctgctggta ttcgcataga
    46201 aggagagtga gatgaccact attaccaaag agcgactgca atggctggct aacatttctg
    46261 gccgcgatga cattgacgat atagacggcg gtgaaattcg tgagctggcg cttatcgcgc
    46321 tggcatcgct ggaagcgaag cctgtagctt catgtattat tgaagatggg ggcatgtgtg
    46381 ttgacgggtt cggtgagtat gttggtcact cgctgcctga tggaacgcac cagctttacg
    46441 ccgccccgcc agtgcctagt aagttgccgc gtgaatacag aaacggttgg cctcttgcat
    46501 atagtgatta tgctgaaggc tggaacgact gccgcgaagc catgcttcag ggagataaat
    46561 catgattaat cgtatcaagc tggagcacat cctcgaatat gccaggcagc agaagcatac
    46621 tggtcagcat tgtaaaattc caccagggga tatggttgaa atcatggaga ttgccatgcg
    46681 caaggctggc aattctccgg taactccgga tggttggata agctgtagtg agcgaatgcc
    46741 ggataccaaa acagccgttc ttgttgccag ggagtttgac aggaaaggtg actggcgaat
    46801 gaaatgggcg acttacatcc cggggcatcc tgacgctaat gatgggtggg tagtacctgg
    46861 tgcgtcgtgg ataccatcac actggatgcc gctaccagaa ccgccgaagg aggtgcgcca
    46921 atgagctggc ctgaggcctt ctcaaatgtt ggtattgcaa tggcggtggc gctggtgggg
    46981 tattcgattt gccgctgggg ataaatcgcc gaaaaaagat cccgccacaa acatgagccg
    47041 ggatctttga tttatatagc ctacgaatcc gcagtaagag aggggcagac ggtttattct
    47101 aacaccggaa tgatgtgggt aaaagtttat aagaaatcgg tttcataact ttgcccacca
    47161 tgatagatac cgacaataaa gacttttctg ccatcaacgg caaaagcaat aatcgttctg
    47221 tggcggaaat gagttacccg catcccctgg cgaatatcat cgcgtttatt gccccgatgc
    47281 gggaatgtag aaaacccatc aagataatca agaagcgcat tggcataatt gtcagcaatg
    47341 atgttccctg ctttctccgt tatatatctg tgcaggttga ttatttgttg ttcggcctca
    47401 ggagtaatga tgacttcata tgtcatgcag attacttccc ggatcgaatc gcgacgcgaa
    47461 cctgtgaaat ggagcgtccg ttgtttgggt tttcgcggat agaatcaaga gagggggcgg
    47521 ctgaatgcgt taaccacgct tcgattgctt tatcgcgctc attcagtgcg cgaagccctt
    47581 cacgaatgac ctcgctttct gaagcatagg cgccggaagc cacacgggcg cgcaccatgt
    47641 cagccatctc gttagttaat gtaatgctga attgttgggt tgtacgcatg gtaaacctca
    47701 cggagtagga tagaacacta ttcgatgata gcacgctgcc tgttgacgac aacagaaatc
    47761 agagacaata ttgccgcacg ccagcctgaa caactggcac ctgctgcgcc agcagagaca
    47821 accgatggcg caagatacca aattacacaa ttcggataac tccaccgccc ctgccagcag
    47881 gcacgggcgg cgttctcatg cattcaaatc tgactggttc cagcatgacc catgcactga
    47941 agaacaggcc gaatggctga tccagtgcta ccgcagacgt ggttacgagt ttcagaaaga
    48001 tctcagcttc gatcgtcgtc actggataat ctccgtcagg ctcccttatt ccgaacgccc
    48061 accgcgtccg tcccgcacat tccagcagcg catctggagg taacgtgcgg gtattacttc
    48121 gacctgttct ggtaccggaa ctcgggatgg tgctccttaa gccgggccgt gaatcaatgt
    48181 cagcattcca taacggcaga atattggtgg aaccggaacc gaaaaacatg cgcggtctgc
    48241 cgtccggagt cgttcctgcc gttcgccagc cgctggcaga ggataaaaca ttactgccat
    48301 ttttcagcga tgagcgggtt attcgtgcag caggtggtgc aggtgcactg tctgactggt
    48361 tattacgtca cgtgaaatcc tgccagtggc cacacggtga ttatcatcac agcgaaaccg
    48421 tcattcaccg ttacggtacc ggcgcgatgg tgttgtgctg gcactgtgac aaccagctgc
    48481 gcgaccagac atcagaatca ctcgatcaac ttgctcagca gaatctggtt gcctggatga
    48541 ttgacgtcat ccgtcacgca ataagcagta cgcaggagag ggagttatcg ctggccgaat
    48601 tatcctggtg ggcggtctgc aatcaggtgg tggatgcact acctgaggca gtagtgcgtc
    48661 gttcgctggg attaccagcg gaaaaaatcc gctccgtata ccgtgagagc gacatcatac
    48721 cgggagagca ggccgccacc agcatactga agcagcgcac aaaaaatatt gcgctgccac
    48781 ttcacgtcca ccagcaacaa aatccccccc aggaaaagac gttagtaagc atcgccgttg
    48841 atccggagtc tccggctcag tacctccagc gccagaaacc acaacgggaa gagatgcctg
    48901 tatacacgcg ctgggtaaaa acgcagaaat gcatgacgtg tggcaatcag gcagatgatc
    48961 cgcatcacat cattggtcat ggactgggag gtatgggaac aaaggctgac gatttgtttg
    49021 ttattccgtt atgccgtaaa tgccataacg aactacacgc cggggtaaaa gattttgaag
    49081 agaaacacgg cagtcagctg ttgttgctga ttcgtttttt aatgcacgcg agaaattcgg
    49141 gtgttctgaa gtggaaagca taaatgaccg agcgcataga atttgttttg ccttacccgc
    49201 cgacggtgaa cacttactgg cgacgccgtg gcagcacata ttttatatcg gaggagggaa
    49261 agcgctatcg ccgggcagtg gcgcttattg ttcgccagca gcggctgaaa ttaagcctgt
    49321 ccggacggct ggcaataaaa attattgcag agccaccgga taagcgtcgt cgtgacctgg
    49381 acaatattct gaaagcaccg ctggatgcgc tgacgcatgc gggagtgctt attgatgacg
    49441 agcagtttga tgaaatcaat attgtacgtg gcaagccagt atctggtgga cggctggggg
    49501 tgaagattta ccccataatg cttgaagggc aggtcaaaaa atgaaactgg aagatttacc
    49561 gaaatactac tccccaaaat cccccggcct gactgatgca tcggcctcaa cgtcgaaaga
    49621 tgcgctgagt atcactgatg tgatggccgc gcagggcatg acacagaatc gggctgagat
    49681 ggggttttct gcgttccttg ggaaaatggg cattagtatg aatgacagag aacgggcaac
    49741 agaattgctg acagaatatg cactcagtcg gtgtgattgc gtggcggcgt taagaaaact
    49801 cccggcagaa ataaaaccgg cagtgatgcg tattatggct tcgtatgcgt ttgaagatta
    49861 tgcccgtagc gcggcgagca aaaaacagtg ctcctgttgt catggaaaaa aatttattga
    49921 aagcgaggtt tttacaaaca agatccagta tccggatggt aagccgccag tgtgggcaaa
    49981 gtgcacaaaa ggcgtgtatc cgtcttactg ggaggaatgg aaaaaagtca gggaggtggt
    50041 aaaagttgcc tgtccggagt gtggcggaaa gggggaggtt tccaccgcct gtaaagattg
    50101 tcgtgggcgc ggtgttgcca ttcatcgtga agagtctgaa aaacggggta tgcctgttat
    50161 cagggactgc cagcgttgtg gtggtcgtgg ctatgaaaga ttgccgtcaa cagaggcatt
    50221 taatgctata tgcgaggtga caaaccagat aacacgcgcg tcatgggaaa aaacagttaa
    50281 gaaattctat gatgcgctgg tgagcaagtt tgatattgag gaagcatggg ctgagaggca
    50341 gttaaaaaag gtaaccaggt aacaaggttg atttttccgg aatctgtggt aaatttgccc
    50401 taacgatggg cgttttatgc ctgacgttag aagacttttt acacccgtcg ccaggcgggt
    50461 ttttttatga ctgaaatcac tccagtacag taaacgcgct ggcggcggtg aataccggtc
    50521 tttcagcttg ctggcttttt cgacaagagt tattggtgtg tcacgttaac cggaaaaggg
    50581 aaaaagacat gctgaaacag caggatatga ccgaaaccgc cagagtggtg tttaatgaat
    50641 taagcgtcac cgaaccggcg acagtcgggg agattgcgca gaatatgtac ctttcacgcg
    50701 aacgctgtca gttaatactg acccagcttg ttatggcggg tctggcagac tatcagttcg
    50761 gttgttacag acgccttcag tcctgaaggc ttttttattt gtggtaaatg ggcggctggt
    50821 g
//
LOCUS       DAZGBC010000014        50659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGBC010000014 DAZGBC010000000
VERSION     DAZGBC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.014"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..427
                     /gene="eutS"
                     /locus_tag="ACHK1J_000856"
     CDS             92..427
                     /gene="eutS"
                     /locus_tag="ACHK1J_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO7910178.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            440..919
                     /gene="eutP"
                     /locus_tag="ACHK1J_000857"
     CDS             440..919
                     /gene="eutP"
                     /locus_tag="ACHK1J_000857"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO7910179.1"
                     /translation="MKRIAFVGTVGAGKTTLFNVLQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            894..1595
                     /gene="eutQ"
                     /locus_tag="ACHK1J_000858"
     CDS             894..1595
                     /gene="eutQ"
                     /locus_tag="ACHK1J_000858"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO7910180.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESSPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWDNAFFPWTLNYDEIDMVLEGELHVRHAGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1592..2395
                     /gene="eutT"
                     /locus_tag="ACHK1J_000859"
     CDS             1592..2395
                     /gene="eutT"
                     /locus_tag="ACHK1J_000859"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO7910181.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2392..3408
                     /gene="pta"
                     /locus_tag="ACHK1J_000860"
     CDS             2392..3408
                     /gene="pta"
                     /locus_tag="ACHK1J_000860"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7910182.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTE
                     PLMFAAAMVSAGIADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3447..3740
                     /gene="eutM"
                     /locus_tag="ACHK1J_000861"
     CDS             3447..3740
                     /gene="eutM"
                     /locus_tag="ACHK1J_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO7910183.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3847..4134
                     /gene="eutN"
                     /locus_tag="ACHK1J_000862"
     CDS             3847..4134
                     /gene="eutN"
                     /locus_tag="ACHK1J_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO7910184.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4146..5549
                     /locus_tag="ACHK1J_000863"
     CDS             4146..5549
                     /locus_tag="ACHK1J_000863"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO7910185.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5560..6396
                     /gene="eutJ"
                     /locus_tag="ACHK1J_000864"
     CDS             5560..6396
                     /gene="eutJ"
                     /locus_tag="ACHK1J_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO7910186.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRIPLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6386..7573
                     /gene="eutG"
                     /locus_tag="ACHK1J_000865"
     CDS             6386..7573
                     /gene="eutG"
                     /locus_tag="ACHK1J_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO7910187.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVCLYA
                     AAQ"
     gene            7790..9016
                     /gene="eutH"
                     /locus_tag="ACHK1J_000866"
     CDS             7790..9016
                     /gene="eutH"
                     /locus_tag="ACHK1J_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO7910188.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHILGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            9013..10416
                     /gene="eutA"
                     /locus_tag="ACHK1J_000867"
     CDS             9013..10416
                     /gene="eutA"
                     /locus_tag="ACHK1J_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO7910189.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10428..11789
                     /gene="eutB"
                     /locus_tag="ACHK1J_000868"
     CDS             10428..11789
                     /gene="eutB"
                     /locus_tag="ACHK1J_000868"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO7910190.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11810..12697
                     /gene="eutC"
                     /locus_tag="ACHK1J_000869"
     CDS             11810..12697
                     /gene="eutC"
                     /locus_tag="ACHK1J_000869"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO7910191.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCTTTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12707..13366
                     /gene="eutL"
                     /locus_tag="ACHK1J_000870"
     CDS             12707..13366
                     /gene="eutL"
                     /locus_tag="ACHK1J_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO7910192.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13379..13879
                     /gene="eutK"
                     /locus_tag="ACHK1J_000871"
     CDS             13379..13879
                     /gene="eutK"
                     /locus_tag="ACHK1J_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO7910193.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13925..14977
                     /gene="eutR"
                     /locus_tag="ACHK1J_000872"
     CDS             13925..14977
                     /gene="eutR"
                     /locus_tag="ACHK1J_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO7910194.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14983..15882)
                     /gene="hemF"
                     /locus_tag="ACHK1J_000873"
     CDS             complement(14983..15882)
                     /gene="hemF"
                     /locus_tag="ACHK1J_000873"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO7910195.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGTEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRKEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15886..16755)
                     /gene="amiA"
                     /locus_tag="ACHK1J_000874"
     CDS             complement(15886..16755)
                     /gene="amiA"
                     /locus_tag="ACHK1J_000874"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO7910196.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16969..17394
                     /locus_tag="ACHK1J_000875"
     CDS             16969..17394
                     /locus_tag="ACHK1J_000875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO7910197.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17381..17830
                     /locus_tag="ACHK1J_000876"
     CDS             17381..17830
                     /locus_tag="ACHK1J_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO7910198.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGFGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            17891..18466
                     /locus_tag="ACHK1J_000877"
     CDS             17891..18466
                     /locus_tag="ACHK1J_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO7910199.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18562..19461
                     /gene="yfeX"
                     /locus_tag="ACHK1J_000878"
     CDS             18562..19461
                     /gene="yfeX"
                     /locus_tag="ACHK1J_000878"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO7910200.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19519..20823)
                     /gene="pbp4b"
                     /locus_tag="ACHK1J_000879"
     CDS             complement(19519..20823)
                     /gene="pbp4b"
                     /locus_tag="ACHK1J_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO7910201.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20828..22252)
                     /gene="murP"
                     /locus_tag="ACHK1J_000880"
     CDS             complement(20828..22252)
                     /gene="murP"
                     /locus_tag="ACHK1J_000880"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO7910202.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSSQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22256..23152)
                     /gene="murQ"
                     /locus_tag="ACHK1J_000881"
     CDS             complement(22256..23152)
                     /gene="murQ"
                     /locus_tag="ACHK1J_000881"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO7910203.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRKVLDKE"
     gene            23316..24173
                     /gene="murR"
                     /locus_tag="ACHK1J_000882"
     CDS             23316..24173
                     /gene="murR"
                     /locus_tag="ACHK1J_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO7910204.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKQLALEQTCALFDFARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24302..25093
                     /gene="ucpA"
                     /locus_tag="ACHK1J_000883"
     CDS             24302..25093
                     /gene="ucpA"
                     /locus_tag="ACHK1J_000883"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO7910205.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPVSVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25397..26413
                     /gene="cysP"
                     /locus_tag="ACHK1J_000884"
     CDS             25397..26413
                     /gene="cysP"
                     /locus_tag="ACHK1J_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO7910206.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26413..27246
                     /gene="cysT"
                     /locus_tag="ACHK1J_000885"
     CDS             26413..27246
                     /gene="cysT"
                     /locus_tag="ACHK1J_000885"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO7910207.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27246..28121
                     /gene="cysW"
                     /locus_tag="ACHK1J_000886"
     CDS             27246..28121
                     /gene="cysW"
                     /locus_tag="ACHK1J_000886"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO7910208.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            28111..29208
                     /gene="cysA"
                     /locus_tag="ACHK1J_000887"
     CDS             28111..29208
                     /gene="cysA"
                     /locus_tag="ACHK1J_000887"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO7910209.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDNAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29328..30239
                     /gene="cysM"
                     /locus_tag="ACHK1J_000888"
     CDS             29328..30239
                     /gene="cysM"
                     /locus_tag="ACHK1J_000888"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO7910210.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQRAGI"
     gene            complement(30242..30610)
                     /locus_tag="ACHK1J_000889"
     CDS             complement(30242..30610)
                     /locus_tag="ACHK1J_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO7910211.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30715..31566
                     /gene="pdxK"
                     /locus_tag="ACHK1J_000890"
                     /gene_synonym="thiJ"
     CDS             30715..31566
                     /gene="pdxK"
                     /locus_tag="ACHK1J_000890"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO7910212.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLVEA"
     gene            complement(31609..32118)
                     /gene="crr"
                     /locus_tag="ACHK1J_000891"
     CDS             complement(31609..32118)
                     /gene="crr"
                     /locus_tag="ACHK1J_000891"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO7910213.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32159..33886)
                     /gene="ptsI"
                     /locus_tag="ACHK1J_000892"
     CDS             complement(32159..33886)
                     /gene="ptsI"
                     /locus_tag="ACHK1J_000892"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO7910214.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33931..34188)
                     /gene="ptsH"
                     /locus_tag="ACHK1J_000893"
     CDS             complement(33931..34188)
                     /gene="ptsH"
                     /locus_tag="ACHK1J_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO7910215.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34572..35543)
                     /gene="cysK"
                     /locus_tag="ACHK1J_000894"
     CDS             complement(34572..35543)
                     /gene="cysK"
                     /locus_tag="ACHK1J_000894"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO7910216.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDIFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35728..36489)
                     /gene="cysZ"
                     /locus_tag="ACHK1J_000895"
     CDS             complement(35728..36489)
                     /gene="cysZ"
                     /locus_tag="ACHK1J_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO7910217.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36719..37717
                     /gene="zipA"
                     /locus_tag="ACHK1J_000896"
     CDS             36719..37717
                     /gene="zipA"
                     /locus_tag="ACHK1J_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO7910218.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37788..39803
                     /gene="ligA"
                     /locus_tag="ACHK1J_000897"
     CDS             37788..39803
                     /gene="ligA"
                     /locus_tag="ACHK1J_000897"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO7910219.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39805..40023
                     /locus_tag="ACHK1J_000898"
     CDS             39805..40023
                     /locus_tag="ACHK1J_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO7910220.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(40020..41018)
                     /locus_tag="ACHK1J_000899"
     CDS             complement(40020..41018)
                     /locus_tag="ACHK1J_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO7910221.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            41108..42034
                     /locus_tag="ACHK1J_000900"
     CDS             41108..42034
                     /locus_tag="ACHK1J_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7910222.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(42025..42366)
                     /locus_tag="ACHK1J_000901"
     CDS             complement(42025..42366)
                     /locus_tag="ACHK1J_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO7910223.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(42486..42561)
                     /locus_tag="ACHK1J_000902"
     tRNA            complement(42486..42561)
                     /locus_tag="ACHK1J_000902"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42526..42528),aa:Lys,seq:ttt)
     gene            complement(42566..42641)
                     /locus_tag="ACHK1J_000903"
     tRNA            complement(42566..42641)
                     /locus_tag="ACHK1J_000903"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42606..42608),aa:Val,seq:tac)
     gene            complement(42688..42763)
                     /locus_tag="ACHK1J_000904"
     tRNA            complement(42688..42763)
                     /locus_tag="ACHK1J_000904"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42728..42730),aa:Val,seq:tac)
     gene            complement(42808..42883)
                     /locus_tag="ACHK1J_000905"
     tRNA            complement(42808..42883)
                     /locus_tag="ACHK1J_000905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42848..42850),aa:Val,seq:tac)
     gene            43142..44557
                     /gene="gltX"
                     /locus_tag="ACHK1J_000906"
     CDS             43142..44557
                     /gene="gltX"
                     /locus_tag="ACHK1J_000906"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO7910224.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(44609..45001)
                     /locus_tag="ACHK1J_000907"
     CDS             complement(44609..45001)
                     /locus_tag="ACHK1J_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7910225.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(45003..45362)
                     /locus_tag="ACHK1J_000908"
     CDS             complement(45003..45362)
                     /locus_tag="ACHK1J_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7910226.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            45583..45658
                     /locus_tag="ACHK1J_000909"
     tRNA            45583..45658
                     /locus_tag="ACHK1J_000909"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45616..45618,aa:Ala,seq:ggc)
     gene            45698..45773
                     /locus_tag="ACHK1J_000910"
     tRNA            45698..45773
                     /locus_tag="ACHK1J_000910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45731..45733,aa:Ala,seq:ggc)
     gene            45981..48170
                     /locus_tag="ACHK1J_000911"
     CDS             45981..48170
                     /locus_tag="ACHK1J_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO7910227.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(48220..49422)
                     /gene="nupC"
                     /locus_tag="ACHK1J_000912"
     CDS             complement(48220..49422)
                     /gene="nupC"
                     /locus_tag="ACHK1J_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO7910228.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            49758..>50659
                     /locus_tag="ACHK1J_000913"
     CDS             49758..>50659
                     /locus_tag="ACHK1J_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO7910229.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLS"
BASE COUNT        11832 a        12848 c        14036 g        11943 t
ORIGIN      
        1 cctcaccttt taaacgcgct tgccgaattt tgttatttac tctgacgaaa aattgtcacg
       61 atacacgcaa gtttttcaca ggcggcgact catggataaa gaacgcatca ttcaggaatt
      121 tgtgcccggc aaacaggtca cgctggcgca tctcatcgcg catcccggcg aggaactggc
      181 gaagaaaatc ggcgttccgg atgcgggcgc aattggcatt atgacgctaa ctcctggcga
      241 aacggcgatg atcgccggtg atttagcgct gaaagctgcc gatgtgcata tcggttttct
      301 cgaccgtttt agcggcgcgc tggtgattta tggctcggtg ggggcagtag aagaagcgtt
      361 atcacaaacc gtcagtggtt taggtcgctt attaaattat acgctttgtg aaatgaccaa
      421 aagttaatac gaggcattca tgaaacgaat tgcgtttgtg ggtaccgtcg gggcagggaa
      481 aacaacgtta tttaatgtgt tgcaggggaa ttataccctc gccagaaaaa cacaggccgt
      541 ggaatttaat gataagggcg atattgatac gccgggtgaa tattttagcc atccccgctg
      601 gtatcacgcc ttaattacca cgctacagga tgtggacatg ctgatctatg tccacggcgc
      661 gaatgacccg gaaagtcggt tgcctgccgg gttgctggat attggcgtca gcaaacggca
      721 aatcgccgtc atcagtaaaa cggacatgcc ggatgctgac gtcgccgcaa cgcgaaagtt
      781 attgcttgag acgggcttcg aggagccgat tttcgagcta aacagccacg acccgcaaag
      841 cgtgcagcaa ctggtggatt atctggcatc tctcaccaaa caggaggaag caggtgaaaa
      901 aactcatcac agcgaatgat attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg
      961 tggttctgcg cgccagcatt attaccccgg aagcccgtga agtggcggat ctactgggtt
     1021 ttaccatcac cgaatgtgat gaatcgtccc cggtaacggc gtccgttccc gccagcgtgc
     1081 cagccgataa aaccgaaagt cagcgcatcc gcgaaaccat cattgcccag ctgccggaag
     1141 gccagtttac cgaaagcctg gtcgcgcagt tgatggaaaa agtgatgaag gaaaaacagt
     1201 cgctggagca gggtgcaatg cagccgagct tcaaatcggt gaccggcaaa ggcggcatca
     1261 aagtgattga tggtagcagc gtcaagtttg gtcgtttcga cggcgcagag ccgcattgcg
     1321 tgggcttaac tgaccttgtg accggagacg acggcagcag catggccgcc ggttttatgc
     1381 agtgggacaa cgcattcttc ccgtggaccc tgaactacga cgaaatcgat atggtgctgg
     1441 aaggggagct gcatgttcgc cacgcaggag aaaccatgat cgccaaagcg ggcgatgtga
     1501 tgtttatccc gaaaggctcc agcatcgaat ttggtacgac atccagcgtg aaattcctgt
     1561 atgtcgcctg gccggctaac tggcaatccc tatgaaagat ttcatcaccg aagcatggct
     1621 aagagcgaac catacgctca gcgaaggcgc agagatccat ctccctgcgg acagtcgcct
     1681 gacgccctct gcccgggagt tactggaaag ccgccatctg cgcatcaagt ttattgacga
     1741 gcagggccgc ctgtttgttg acgatgagca gcagcagcca cagcccgttc acgggctgac
     1801 cagtagcgat gaacatccgc aggcgtgctg cgaactgtgc cgccagccgg tggcaaaaaa
     1861 gccggatacg ctgactcatc tgtcggcgga aaaaatggtc gctaaaagcg atccgcgtct
     1921 gggttttcgc gcggttctcg acagcaccat tgcgctggca gtgtggctgc aaattgaact
     1981 ggcggaaccg tggcagccgt ggctggcgga tatccgttcg cgtcttggca acattatgcg
     2041 cgccgatgcg ctgggggaac cgctgggctg ccaggcgatt gtcgggcttt ctgacgaaga
     2101 tctgcaccgg ctttctcatc agccgttgcg ttatcttggt cacgatcatc tggtgccgga
     2161 agccagccac gggcgcgatg cggcgttgct caatctgcta cgcaccaaag tgcgggaaac
     2221 cgaaaccgta gcggcgcagg tgtttatcac ccgcagcttt gaagtattac gcccggacat
     2281 cttgcaggcg ctgaaccgcc tttcgagcac ggtctacgtg atgatgatcc tgagcgtaac
     2341 gaagcagccg ctaacggtga agcagatcca acaacgactg ggggaaacgc aatgattatt
     2401 gaacgttgtc gtgaactggc gttgcgagcg cccgccagag tggtttttcc ggatgcgtta
     2461 gatcaacgtg tgctgaaagc ggcgcaatat ttacatcaac aaggtctggc aacgcccatt
     2521 ctggtcgcca atccgtttga acttcgtcag tttgcgctca gtcatggcgt agcgatggac
     2581 gggctacagg tgattgatcc gcatggcaac ctcgcaatgc gggaagaatt tgctcatcgc
     2641 tggctggccc gcgcgggcga aaaaacgccg ccggatgcgc tggaaaaact caccgaaccg
     2701 ctgatgttcg ccgccgcaat ggtcagcgcc ggtatagcgg atgtctgtat cgcgggcaac
     2761 ctctcttcca cggcgaatgt gctgcgtgcc ggattacgca ttatcggctt gcagccaggc
     2821 tgtaaaacgc tctcatccat tttcctgatg ctgccacagt acagtggccc cgcgttgggc
     2881 tttgccgatt gcagcgtggt accacagccg acggcggcgc agctggcgga tatcgcgctt
     2941 gccagcgccg aaacctggcg cgccatcacc ggagaagagc cgcgcgtggc gatgctgtcg
     3001 ttttccagta acggtagcgc ccgtcacccc tgcgttgcca acgtccagca ggcgacagaa
     3061 atcgtccgtg agcgcgcacc aaagctggtt gtcgatggcg agttgcagtt tgacgccgcc
     3121 ttcgtgccgg aagtggcggc gcaaaaagcg cctgccagcc cgctacaggg caaggccaat
     3181 gtgatggttt ttccgtcgct ggaagccgga aatattggtt acaaaatcgc acagcgactt
     3241 ggcggatatc gtgccgtcgg gccattgata caaggacttg ccgcgccgat gcacgatctc
     3301 tctcgtggtt gtagcgtgca ggaaattatc gagctggcgc tggtggcagc tgtgccgcgt
     3361 cagacagaag tgaaccgcga aagcagttta caaacactgg ttgaatgaac ggtcccgttc
     3421 tggaccctga taaagaggaa agcacaatgg aagcattagg aatgatcgaa acccggggcc
     3481 tggttgcact gattgaggct tctgacgcca tggtgaaagc tgcgcgcgtc aagctggttg
     3541 gtgttaagca gattggcggc ggcctgtgta ctgccatggt tcgtggtgat gttgcagcct
     3601 gtaaagcggc gaccgatgca ggtgccgcag cggcacagcg tatcggcgag ctggtatctg
     3661 tacacgttat cccgcgtccg cacggcgacc tggaagaagt gttcccgatc ggcctgaaag
     3721 gcgatagcag caacctgtaa tcaccctgtg tgacgtcgat tcgggcgtca cgctctctca
     3781 tttttcattt taccttccgc ggagggtagg gggagctttt acctgaaata cgccacggag
     3841 gcgggtatga aactggcagt cgtcactgga caaattgttt gtaccgtacg ccatcacgga
     3901 ctggcgcatg acaaattgct gatggtggaa atgattgatc cacatggtaa tcctgacggg
     3961 caatgtgccg tcgccatcga caatattggc gcgggaaccg gggagtgggt gttgctggtg
     4021 agtggcagtt ccgcccgcca ggcgcataaa agcgaaacgt caccggtcga tctgtgcgtg
     4081 attggcatcg tcgatgaggt ggtgtctggc ggtcaggtaa ttttccacaa ataaggcaga
     4141 acatcatgaa tcaacaggat attgaacagg tggtgaaagc ggtactgctg aaaatgcaaa
     4201 gcagtgacac gccgcccgcc gccgttcatg agatgggcgt tttcgcgtcc ctggatgacg
     4261 ctgttgcggc agccaaagtc gcccagcaag ggttaaagag cgtggcaatg cgccagttag
     4321 ccattgctgc cattcgtgaa gcaggcgaaa aacacgccag agatttagcg gaacttgccg
     4381 tcagtgaaac cggcatgggt cgcgttgaag ataaatttgc caaaaacgtc gctcaggcac
     4441 gcggcacgcc aggcgttgag tgtctctctc cgcaagtgct gacgggcgac aacggcctga
     4501 ctttaattga aaacgcgccc tggggcgtgg tggcttcggt gacgccgtcc actaacccgg
     4561 cggcaaccgt aattaataac gccatcagcc tgattgccgc gggcaacagc gtcatttttg
     4621 ccccgcatcc ggcggcgaaa aaagtctccc agcgggcgat cacgctgctc aaccaggcga
     4681 ttgttgccgc aggcgggccg gaaaacttac tggttactgt ggcaaatccg gatatcgaaa
     4741 ccgcgcaacg cttgttcaag tttccgggta tcggcctgct ggtggtgacc ggcggcgaag
     4801 cggtagtaga agcggcgcgt aaacacacta ataaacgtct gattgccgca ggcgctggca
     4861 acccgccggt agtggtggat gaaaccgctg acctcgcccg tgctgctcag tccatcgtca
     4921 aaggcgcgtc tttcgataac aacatcattt gtgccgacga aaaggtactg attgttgttg
     4981 atagcgtagc cgatgaactg atgcgtctga tggaaggcca gcacgcggtg aaactgaccg
     5041 cagaacaggc gcagcagctg caaccggtgt tgctgaaaaa tatcgacgag cgcggaaaag
     5101 gcaccgtcag ccgtgactgg gttggtcgcg atgcggcgaa aatcgcggcg gcaatcggcc
     5161 ttaaagttcc gcaagaaacg cgcctgctgt ttgtggaaac caccgcagaa catccgtttg
     5221 ccgtgactga actgatgatg ccggtgttgc ccgtcgtgcg cgtcgccaac gtggcggatg
     5281 ccattgcgct agcggtgaaa ctggagggcg gttgccacca cacggcggca atgcactcgc
     5341 gcaatatcga gaacatgaac cagatggcga atgccattga taccagcatt ttcgttaaga
     5401 acggaccgtg cattgccggg ctgggtctgg gcggggaagg ctggactacc atgaccatca
     5461 ccacgccaac cggtgaaggg gtaaccagcg cgcggacgtt tgtccgtctg cgtcgctgtg
     5521 tattagtcga tgcgtttcgc attgtttaag gagtaacaga tggcgcacga cgaacaatgg
     5581 ctcaccccac gtctgcaaac ggcggcaacg ctgtgtaacc agacgcccgc cgcgacggaa
     5641 tctccgctgt ggctgggggt ggatctgggc acctgcgatg tggtgtcgat ggttgtcgac
     5701 cgcgacggtc agccagtggc ggtatgcctc gactgggccg atgtggttcg cgacggcatc
     5761 gtctgggatt tcttcggcgc ggtcaccatt gttcgtcgcc atctcgacac gctcgagcag
     5821 caattcggtc gtcgttttag ccatgcggcg acttcatttc ctccaggtac ggacccgcgt
     5881 atttccatca acgtgctgga atctgccgga ctggaagtga gccatgtgct ggatgagcca
     5941 accgcagtcg cggatctgct gcaactggac aacgccggtg tagtggatat cggcggcggc
     6001 actaccggca ttgccatcgt gaaaaagggc aaggtgacgt actcggcgga tgaagcgact
     6061 ggcggacatc acatctctct gactctcgcc ggaaatcgtc gtattccgct ggaagaggca
     6121 gagcagtaca agcgcggtca cggtgacgag atttggcccg cggtgaaacc ggtgtacgaa
     6181 aaaatggcgg acattgtcgc ccggcatatt gaagggcagg gcattactga tttatggctg
     6241 gcgggcggct cttgtatgca accgggcgtg gcagaactgt ttcgcaaaca attcccggcg
     6301 ttacaggtgc atttaccgca gcacagtttg tttatgaccc cgctggcgat cgccagtagt
     6361 gggcgagaga aagcggaggg gctatatgca aaatgaattg cagaccgcgc tctttcaggc
     6421 gttcgatacc ctgaatctgc aacgggtaaa aacatttagt gttccaccgg tgacgctttg
     6481 tggtccgggc tcggtgagca gttgcggaca gcaagcgcaa acgcgtgggc tgaaacatct
     6541 gttcgtgatg gcagacagct ttttgcatca ggcagggatg accgccgggc tgacgcgtag
     6601 cctggccgtt aaaggtatcg ccatgacgct ctggccatgt ccggtgggcg aaccgtgcat
     6661 taccgacgtg tgtgcagccg tggcgcagtt gcgtgaatca ggttgtgacg gggtgatcgc
     6721 atttggcggc ggctcggtgc tggatgcggc gaaagccgtg gcgttgctgg tgacgaaccc
     6781 ggatagcacg ctggcagaga tgtcagaaac cagcgttctg caaccgcgct tgccgctgat
     6841 tgccattcca actaccgccg gaaccggctc tgaaaccaca aatgtaacgg tgattatcga
     6901 cgcggtgagc gggcgcaagc aggtgttagc ccatgcctcg ctgatgccgg atgtggcgat
     6961 cctcgacgcc gcattgaccg aaggtgtgcc gtcgcatgtc acggcgatga ccggcattga
     7021 tgcgttaacc catgccattg aagcatacag cgccctgaac gctacaccgt ttaccgacag
     7081 tctggcgatt ggtgccattg cgatgattgg caaatcgctg ccgaaagcgg tgggctacgg
     7141 tcacgacctt gccgcgcgcg agagcatgtt gctggcttca tgtatggcgg gaatggcgtt
     7201 ttccagtgcg ggtcttgggt tgtgccacgc gatggcgcat cagccgggcg cggcgctgca
     7261 tattccgcac ggtctggcga acgctatgtt gctgccaacg gtgatggagt ttaaccggat
     7321 ggtttgtcgc gaacgcttta gtcagattgg tcgggcactg cgaactaaaa aatccgacga
     7381 tcgtgacgct attaacgcgg taagtgagct gattgcggaa gttgggattg gtaaacgact
     7441 gggcgatgtt ggcgcgacat ctgcgcatta cggtgcatgg gcgcaggccg cgcaggaaga
     7501 tatttgtctg cgcagcaacc cgcgtaccgc cagcctggag cagattgtct gcctgtacgc
     7561 agcggcgcaa taaatgccgg atgcgacgct tgccgcgtct tatccggcct acgggattgc
     7621 acatgtagag cggataaagc gttcacgccg catccgccag tggcgcggtg caattgccgg
     7681 atgcgacgct tgccgcgtct tatccggcct acgggattgc acatgtaggg cggataaggc
     7741 gttcacgcag catccgctaa taaataacaa ctaacaggga gtaaaggcga tgggaattaa
     7801 cgaaatcatc atgtacatca tgatgttctt tatgctgata gctgccgtag acaggatcct
     7861 gtcacagttc ggcggttctg ctcgtttcct cggtaagttc ggtaaaagta tcgaaggatc
     7921 aggcggtcag ttcgaagaag gctttatggc aatgggcgca ctgggcctgg cgatggtcgg
     7981 tatgaccgcg ctggcaccgg tactggctca catactcggg ccggtaatta tcccggttta
     8041 cgaaatgctc ggcgcaaacc cgtcaatgtt cgccggaacg ctgctggcgt gcgatatggg
     8101 cggcttcttc ctcgccaaag agctggcggg cggcgacgta gcagcgtggc tatactcagg
     8161 gttaattctc gggtcgatga tggggccaac gattgtgttt tccattccgg tggcgctcgg
     8221 cattatcgaa ccttctgacc gtcgttatct ggcgctcggc gtgctggcgg gcattgtgac
     8281 cattccgatt ggctgtattg ctggtggtct ggtagctatg tactccggtg tgcagatcaa
     8341 cggccagccg gtggaattca ctttcgccct gatcctgatg aacatgatcc cggtgatcat
     8401 tgttgcgatt ctggtggcgc tggggctgaa attcatcccg gaaaaaatga tcaacggctt
     8461 ccagatcttc gccaaattcc tcgttgcatt gatcaccctc ggtcttgccg ctgcggtagt
     8521 gaaattcctg cttggctggg aactgatccc cggtctggat cctatcttta tggcccctgg
     8581 cgataaaccc ggtgaggtga tgcgcgccat tgaagttatc ggttctatct cctgcgttct
     8641 gttaggggcg tatccgatgg tgctgctgct gacccgctgg tttgaaaaac cgctgatgag
     8701 cgtcggtaaa gtgctgaata tgaacaacat cgcggcagcc ggcatggtgg caacgcttgc
     8761 caacaacatc ccgatgttcg gcatgatgaa gcagatggat acccgcggca aagtcatcaa
     8821 ctgcgccttc gccgtttccg ctgctttcgc cctgggcgac cacttaggct tcgccgctgc
     8881 caacatgaat gcaatgatct tcccgatgat tgtcggcaag ttgatcggcg gcgtaacggc
     8941 gattggcgtg gcgatgatgc tggtgccaaa agacgacgcg accgcggcta aaaccgaagc
     9001 ggaggcacaa tcgtgaacac tcgccagcta ctgagcgtcg gtatcgatat cggcaccacc
     9061 accacccagg tgattttctc ccacctggaa ctggttaacc gtgcggcggt gtcgcaggtg
     9121 ccgcgctacg aattcatcaa acgcgaaatt agctggcaaa gcccggtgtt ctttacccct
     9181 gtcgataaac agggtggttt aaaagaagcg gaactgaaat cgctgatttt agagcaatat
     9241 caggctgcgg gcattgcgcc ggaaagcgtt gattctggtg ccatcatcat caccggtgaa
     9301 agcgcgaaaa cccgcaatgc tcgcccggcg gtgatggcgc tctctcagtc gctgggcgat
     9361 tttgtcgttg ccagcgccgg gccgcacctc gaatccgtga tcgccggtca cggagccggg
     9421 gcacaaaccc tttctgaaca acggctgtgt cgggtactga atatcgacat cggcggtggc
     9481 accgcgaact acgccctgtt cgatgccgga aaaatcagcg gcactgcctg cctcaacgtc
     9541 ggtggtcgcc tgctggaaac cgacagccag gggcgcgtgg tttacgctca taaaccgggg
     9601 cagatgattg tggatgagtg cttcggcgca ggcaccgacg cccgttcgct gaccggcgct
     9661 cagctggtgc aggttacccg gcggatggca gagctgattg tcgaagtgat tgacggaacg
     9721 ctttcgccgc tcgcgcaggc attaatgcaa accggtttgc tgcccgcagg tgttacgccc
     9781 gaaatcatta cgctttctgg cggcgtgggc gaatgttatc gccaccagcc cgccgacccg
     9841 ttctgttttg ccgatattgg cccgctgctg gcaacggcgc tgcatgacca tccgcgcctg
     9901 cgtgagatga acgtacagtt tccggcgcaa accgtgcgcg ccacggtgat tggtgcgggt
     9961 gcacataccc tttcgctctc tggcagcaca atctggcttg agggcgtaca actgccgctg
    10021 cgcaatttgc cggtggcgat cccaattgat gaaacggatc tggtgagtgc ctggcaacag
    10081 gcgctgcttc agctggatct tgatcccaaa actgacgcgt acgtgctggc gcttcccgcc
    10141 tcgctgcctg tgcgttacgc cgcggtactg acggtcatca acgcgttggt cgatttcgtc
    10201 gcgcgttttc cgaatccgca tcccctgctg gtggtggccg ggcaggactt tggtaaagct
    10261 ctgggcatgt tgttgcgccc acagctacaa caactcccgt tggcagtcat tgacgaagtg
    10321 attgtccgcg cgggggacta tatcgacatt ggtacgcctc tttttggcgg atcggttgtg
    10381 ccggtgacgg tgaaatcact cgcatttcct tcctgaggga acgacttatg aaactaaaga
    10441 ccacattgtt cggcaatgta tatcagttta aggatgtaaa agaggtgctg gctaaagcca
    10501 acgaactgcg ttcgggggat gtgctggcgg gcgtcgcagc ggcaagctca caggagcgcg
    10561 tggcggcaaa gcaggtgttg tcggaaatga ccgtagcgga catccgcaat aatccggtga
    10621 ttgcctatga agatgactgc gtgacgcggc tgattcagga cgatgttaac gaaacggcct
    10681 acaaccagat taaaaactgg agcatcagcg aactgcgtga gtatgtgctg agcgatgaaa
    10741 ccagcgtgga cgacattgcc tttacccgca aagggctgac ctcggaagtg gtcgcggcgg
    10801 tagcgaagat ttgctccaac gcggacctga tctacggcgc gaagaaaatg ccggtaatca
    10861 aaaaggccaa taccaccatc ggtattccgg gcacctttag cgcccgtttg cagccaaatg
    10921 acacccgtga cgacgtgcaa agtatcgccg cgcaaatcta cgaagggctt tccttcgggg
    10981 tgggcgatgc ggtgatcggc gttaacccgg tgactgacga tgtggaaaac ttaagccgcg
    11041 tgctggatac catttatggc gtgatcgaca aattcaacat cccaactcag ggctgcgtac
    11101 tggcgcacgt caccacccag atcgaagcga ttcgtcgcgg cgcgcctggc ggactgattt
    11161 tccagagtat ctgtggcagc gaaaaagggc tgaaagagtt tggcgtggaa ctggcgatgc
    11221 tcgacgaagc gcgcgcagtg ggcgcagagt tcaaccgtat cgccggggaa aactgcctct
    11281 acttcgaaac cggacaaggc tctgcgctat ccgctggcgc taacttcggc gctgaccagg
    11341 tgacgatgga agcacgtaac tatgggctgg cgcgtcatta cgatccgttt atcgtcaaca
    11401 ccgtggtcgg ttttattggg ccggagtatc tctacaacga ccgccagatt atccgtgcgg
    11461 gcttagaaga tcactttatg ggcaagctga gcggcatctc tatgggctgt gactgctgtt
    11521 acaccaacca cgctgacgct gaccagaacc tcaacgaaaa cctgatgatc ctgctcgcca
    11581 ccgcaggctg caactacatc atggggatgc cgttgggcga tgacatcatg ctcaactacc
    11641 agaccaccgc attccacgat accgccactg tgcgtcagtt actcaacctg cgtccgtcac
    11701 cggagtttga acgctggctg gaaagcatgg gcattatggc aaacggtcgc ctgaccaaac
    11761 gggcgggcga tccgtcactg ttcttctgat gacgcggaga taacacatca tggatcaaaa
    11821 acagattgaa gaaattgtac gcagcgtgat ggcgtcaatg ggacaaacgg cccccgcgcc
    11881 gtcagaagca aagtgcacca ccaccacctg tgcggcaccg gtgacctcgg aaagctgcgc
    11941 gctggattta ggttctgcgg aagcaaaagc gtggattggt gttgaaaacc cgcatcgcgc
    12001 agacgtatta acagaactgc gccgcagcac cgtggcccgc gtttgtaccg gtcgtgccgg
    12061 tccgcgtcca cgtacgcagg cgctgctgcg tttcctggcc gatcactctc gttcgaaaga
    12121 taccgtactg aaagaagtgc cggaagagtg ggtgaaagca caaggcttgc tggaagtccg
    12181 ctccgaaatt agcgacaaaa atctctacct gactcgcccg gatatgggcc gccgcctgtg
    12241 tgcagaagct gttgaagcgc tgaaagcgca gtgtgttgcc aatccggacg tgcaggtcgt
    12301 tatttctgat ggcctgtcaa ctgatgcgat caccgtgaac tacgaagaga tcctgccacc
    12361 gctgatggcg ggcctgaaac aggccggtct gaaagtcggt acaccattct ttgttcgtta
    12421 tggtcgcgtg aagattgaag atcagatcgg tgagatcctc ggcgcgaaag tagtgatcct
    12481 gctggtgggc gaacgtccgg ggttgggtca gtcagaaagc ctctcctgct acgccgttta
    12541 ctcgccgcgt atggcgacca ccgttgaggc cgatcgcacc tgcatctcta acattcacca
    12601 gggcggcacg ccgccggttg aagccgcagc cgtcattgtg gatttggcga aacgtatgct
    12661 ggagcagaaa gcatccggca tcaacatgac ccgataagga ggcatcatgc cagctttaga
    12721 tttgattcga ccgtcggtaa ccgccatgcg ggtgattgcc tccgttaacg ccgattttgc
    12781 gcgtgaactg aaattgccgc cgcatattcg tagcctcggt ctgatttctg ccgactccga
    12841 tgacgtcacc tacattgccg ctgatgaagc gaccaagcag gcgatggtgg aagtggtgta
    12901 tggccgctcg ctgtatgcgg gcgctgcaca cggcccgtca ccgactgccg gtgaagtgct
    12961 gattatgctt ggtgggccaa acccggcgga agtgcgtgcc ggtctggatg cgatggttgc
    13021 gcatattgaa aatggcgctg ctttccagtg ggctaatgat gcgcaagata cggcattcct
    13081 ggcacatgta gtttcgcgta ccggttctta tctctcatca accgccggga tcacgcttgg
    13141 cgatccgatg gcgtatctgg tggcaccgcc gctggaagcg acctacggca ttgatgcagc
    13201 gttgaaatct gccgacgtac agctggtgac ctatgtcccg ccaccgtctg aaactaacta
    13261 ctcggcagca tttttaaccg gtagccaggc agcgtgtaaa gcggcctgta acgcctttac
    13321 cgatgcagtg ctggaaatcg cgcgtaatcc aatccagcgt gcgtaatgga ggttgccgat
    13381 gatcaatgca ctgggattgc tggaagtgga cggcatggtc gctgcgatag atgcagcgga
    13441 tgccatgctc aaagcagcta acgttcgtct gctcagtcac gaagtgcttg accctggtcg
    13501 cttaacgctg gtggtggaag gcgatctggc ggcgtgtcgt gctgcgctgg acgcaggttg
    13561 tgctgccgcg atgcgtaccg ggcgtgtcat cagccgcaag gagatcggtc ggccagacga
    13621 tgacacccag tggctggtta ctggctttaa ccgccagccg aagcaacccg taaaggaacc
    13681 cgacgcgcca gttatcgtcg cggaatctgc tgacgagttg ttggcgctgt taacatcagt
    13741 acgtcaggga atgacggcag gagaagtggc tgcccacttt ggctggccgc tggaaaaagc
    13801 cagaaatgcg ctcgaacagc tcttttctgc cgggacgtta cgtaaacgca gtagtcgtta
    13861 tcgtctcaag ccccattaac ctgtcggagg tgccgggtgt cctacaacac ccggcattaa
    13921 catcatgaaa aagacccgta cagccaattt gcaccatctt tatcatgaac ccttacccga
    13981 aaacctgaag ctcacgccga aggtcgaagt ggataatgtt catcaacgac agacaacgga
    14041 tgtctatgaa catgctttaa cgattaccgc ctggcagcag atttacgatc agctgcatcc
    14101 gggcaagttt catggtgaat ttacggaaat tctactcgat gatattcagg tttttcgtga
    14161 atacaccggt ctggcgctgc gtcagtcgtg cctggtctgg cctaactcgt tctggtttgg
    14221 cattccggcg acgcgcggtg agcagggatt tatcggttcg caatgtctgg gaagcgcaga
    14281 aatagcgacg cgccccggtg gaaccgagtt tgaattaagt acgccggacg attacacgat
    14341 cctgggcgta gtgctgtctg aagatgtcat cacccgtcag gctaactttt tgcacaatcc
    14401 ggatcgggta ttacatatgc tgcgtagcca atcggcgctg gaagtgaaag agcagcataa
    14461 agccgcgctg tggggctttg tccaacaggc gctggcgacg ttttgcgaga acccggaaaa
    14521 tctccatcag cctgcagtgc gaaaagtgct gggggataat ttgctaatgg cgatgggggc
    14581 gatgctggaa gaagcgcaac caatggtgac ggcggaaagc atcagtcatc agagttaccg
    14641 tagattactt tcccgcgccc gtgaatatgt gctggaaaac atgtccgaac cggtgacggt
    14701 gctggacttg tgtaatcaac tgcatgtcag ccgccgcacg ctacaaaacg cgtttcacgc
    14761 tattttaggc attggcccga acgcgtggct gaaacgcatt cgcctgaacg ccgtacgccg
    14821 cgaactgata agcccgtggt cgcaaagcac aacggtaaaa gacgccgcca tgcagtgggg
    14881 attctggcat ctggggcaat ttgccacgga ttaccagcag ctgtttgccg agaagccgtc
    14941 gttgacgttg catcagcgga tgcgggaatg ggggtgaggg agttacaccc aatccctgac
    15001 cttaataaac tcacttaacg ccgcttctgg gctgccatct tttggctgat aatcatactc
    15061 ccagcgtacc agtggcggca ttgacatcag gatcgactcg gtgcgcccgc cagtttgcag
    15121 gccaaacaga gtgccgcgat cccagaccag attgaactcg acataacgac cgcgacggta
    15181 gagctgaaaa ttgcgctcgc gctcgccgta ggccatcgct ttccgtcgct ctacaattgg
    15241 taaataagcg tcggtgtagc ctttgcctac cgcctgcata aaggcaaaac agtggtcgaa
    15301 atctggcgtg ttcagatcat caaagaacag cccgccaatg ccgcgctgtt ctttgcgatg
    15361 tttaaggtag aagtattcgt cgcaccactt tttgtaacgg ggataaacgt cttcgccaaa
    15421 cggcaggcac aggtcgcggg cggtgcggtg ccagtgaatg gcatcttctt caaaaccata
    15481 gaaaggggtt aaatcgaagc cgccgccaaa ccaccagact ggctctgcac ccggtttttc
    15541 ggcaataaaa aagcgcacat tcgcgtggct ggtgggaaca tacgggttat gcggatgcac
    15601 taccagtgaa acgcccatcg cctcgaaact gcgcccggca agttccgggc gatgagcggt
    15661 ggcggaagca ggcatcgcct caccgtggac gtgagaaaag ttgacgcccg cctgttcgaa
    15721 aacaccacca ttacgcaaca cccgactacg cccgccgccg ccaccttcgc gctgccaact
    15781 atcttcgaca aattctgtgc catcgacggc ggtcaactgc tgacaaatcg tatcctgaag
    15841 gttgagcaga aactgtttaa cctggtgtgc gtcgggtttc ataacttatc gctttttcga
    15901 atgtgctttc tggttgtcga accagtggaa ataactgatc acgccttcag caatcgctgt
    15961 ggcgattttc tgacgaaacg ccgccgtgcc taacagccgc tcttcttccg ggttggtgat
    16021 aaacgaggtt tccaccagca ccgaaggaac cgacggcgat ttcaacacca caaacgcggc
    16081 ttgttcggtg ttgcggctgt gcagtttatg caccggctta atcttcttca gaatatgcga
    16141 gccaagcgtc aggctgtttt taatagtatc ggtttgcacc agatcaaaca gcacttgctg
    16201 caataggtga tccttgtcag tcgccttttt accggcaact tcatcggcgc ggttttcgcg
    16261 ttcagacagg tatttcgcca ttgcgctact tgccccacgg ttagagaggg caaataccga
    16321 agcaccggca gctttcgggt tggtaaagcc atcggcatga attgacataa acagatctgc
    16381 gccatgttta tgggcgattt caacgcgatc gtaaagtggg ataaacgtat cgccagaacg
    16441 cgttaaacgc gcatcaatcc catgattacg caaaatggaa cggacgtttt tagcaatcgc
    16501 cagcaccaca tgtttttctt tcgaaccgtt gcgcccgatc gctccggtat caatcccgcc
    16561 gtggcctgga tcgagaacaa cgacacgttt gccgccagat tttttagctt tcggcttgct
    16621 gtgtccgttg ctggttttta aaggttcgtc tttggcgatg gcttgcgaca ttcctgacaa
    16681 cgtcagggca gccaaaccgg ctttcagcac ctggcggcgc gaagtgagtg tttttagtgg
    16741 tttaaaagtg ctcatacggc ctgagttgta ataataaagt tccagatgtt atatcgtatc
    16801 gcgtaatccg ttacgaccgt ttgctgttga gaattgtttt acttttcatt tcaatagttg
    16861 acaactcccc attatgccac tttttcaacc gattttcgtc agatattggc taattcgacc
    16921 gttcacgcca taatcagcct tttaacccgc aaaaaggtaa cgaataccat ggagatacgc
    16981 gtatttcgcc aggaagattt cgaagaggtc atcacccttt gggagcgttg cgacttgctg
    17041 cgtccgtgga acgatccgga aatggacatc gagcgtaaga tgaaccatga cgtcagtttg
    17101 tttctggtcg ctgaggtgaa cggtgaagtg gtcggaacgg tgatgggcgg ttatgacggg
    17161 catcgcgggt ctgcttatta tcttggcgtg catccagagt ttcgtgggcg tgggattgcc
    17221 aatgcgttgc ttaaccggct ggagaaaaag ctgattgctc gcggctgccc gaaaattcag
    17281 atcaacgtgc cggaagataa cgacatggtg ctcggaatgt atgaacgcct gggatatgaa
    17341 cacgccgacg tgctgagtct gggtaagcgt ttgattgaag atgaagagta ctgagtttca
    17401 tcctgtccat tatgatgcgc acggtcgcct gcgtttaccc ttgctcttct ggcttgtgct
    17461 attgcttcag gcgcgaacct gggtgctgtt tgtcattgcc ggtgcgtcgc gcgagcaggg
    17521 tactgcgctg ctaaatctgt tttatcccga tcacgataat ttctggctgg ggctgattcc
    17581 tggcattccg gcggtgctgg cgtttttgtt gagcggtcgg cgggctacgt ttcctcgcac
    17641 ctggcgtgta ctctattttc tgttgctgtt ggcgcaggtg gttttacttt gctggcaacc
    17701 gtggctgtgg ctgaacggtg aatccgttag cggtttcggt ctggcgctgg tagtggcgga
    17761 tattgtggcg ttaatctggc tgctgaccaa tcgacgttta cgcgcttgtt ttaatgaagt
    17821 aaaagaataa cggcactttt tggtgaattt gcactccaag caacgttatt gaataaccaa
    17881 aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct atgccattga tgctgaccgg
    17941 ctgttccacg atgtcgtcag ttaactggtc tgccgctaac ccgtggaact ggtttgggtc
    18001 gtccaccaaa gtgagcgagc agggcgttgg tgaattaacg gcgtccacac cactgcaaga
    18061 acaagccatt gccgatgcgc ttgatggcga ttatcgcctg cgcagcggaa tgaaaaccgc
    18121 gaacggcaac gtggtgcgct tttttgaagt gatgaagggc gacaacgtgg cgatggtgat
    18181 taacggcgat caaggcacga tcagtcgcat tgacgtgctg gatagcgata ttcctgctga
    18241 caccggtgtt aaaatcggta caccgtttag cgacctttac agcaaagcat ttggcaattg
    18301 ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt aaagccgaag gcagtcaaca
    18361 tattagctac cagttcagcg gggaatggag tggtcctgaa gggttaatgc cttcggacga
    18421 taccctgaaa aactggaaag tcagtaaaat tatctggcgg cgttaatttg cgtctgaaca
    18481 aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca atgccacgga ttgcgtggca
    18541 ttattatttt caggaggaac aatgtctcag gttcagagtg gcattttgcc agaacattgc
    18601 cgcgcggcga tttggatcga agccaacgtg aaaggggaag ttgacgccct gcgtgcggcc
    18661 agtaaaacat ttgccgacaa actggcgact tttgaagcga aattcccgga cgcgcatctt
    18721 ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc tgagcggcgg cgttggggcc
    18781 gaagagctga aagattttcc gggctacggt aaaggccttg cgccaacgac ccagttcgat
    18841 gtgttgatcc acattctttc tttgcgtcac gacgtaaact tctctgtcgc tcaggcggcg
    18901 atggaagctt tcggcgactg cattgaagtg aaagaagaga tccacggttt ccgttgggtt
    18961 gaagagcgtg acctgagcgg ctttgttgac ggtacggaaa acccggcggg tgaagagacg
    19021 cgtcgcgaag tggcggttat caaagacggc gtggatgcgg gcggcagcta tgtgtttgtc
    19081 cagcgttggg agcacaacct gaagcagctc aaccgtatga gcgttcacga tcaggagatg
    19141 atgatcgggc gcaccaaaga ggccaacgaa gagatcgacg gcgacgaacg cccggaaact
    19201 tctcacctga cccgcgttga tctgaaagaa gatggcaaag ggctgaaaat tgttcgtcag
    19261 agcctgccgt acggcaccgc cagtggtacg cacggtctgt acttctgcgc ctactgcgcg
    19321 cgtctgcata acattgagca gcaactgctg agcatgtttg gcgataccga tggtaagcgt
    19381 gatgcgatgt tgcgtttcac caaaccggta accggcggct attatttcgc accgtcgctg
    19441 gacaagttga tggcgctgta agcgtttctg attcattgat taaaggccag gcagtaacat
    19501 tactgacagg cctttttatt acttttgctt taacgccgca tacacctgat caactaccca
    19561 accatacgtt gcaatcggca gctgaccgct ttcgaacata ttgggattct tttgcggatc
    19621 ggcaaccggc gaatgtggct tgttgcttaa catcacaatc gccatatgat tcaccggatc
    19681 gataacggtc accgttccgg tccagccagt gtgaccgtag gtttgcgggc ttgccagcgt
    19741 gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc cagccgaggc caaaagtggc
    19801 atcttcctta gagctggtgg tgaacatctt caccgtttcc gcactgaaca gctgcacatc
    19861 gccatagccc ccgccgttca gcatcgtttg cattaacacc gcaatatcgc cggtattgga
    19921 aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa aaggcttttt catcgtgcac
    19981 ctgaccccag agagtggagg tgcggatatt cgggaagtgg atcactccat cgcgggtatt
    20041 gccgttcaat tccgtggcgg caatttgctg cggtttaaag cctttcagca gcgggttaaa
    20101 taccgtatgt gtcaggccga gcgggcgata aatcgactct tcaacatagc ggtcaagcgg
    20161 ctgaccggtg accgactcga cgataaatcc aagcagcata taatcgacat cactgtaaat
    20221 atgcttgctg ccgggctgat actctagcgg cgtgcgcttg atcatctcca gcgtctggcc
    20281 tttatcctgg gaatataacg cgcccgcgac cgctttattc ggatattgtg ggtccgccgg
    20341 gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagc gtgtttttgc ctttgatggt
    20401 gtcatttgga ctatcggcaa atcccggaat atatttcgca atccgatcgt caggatgcag
    20461 cttgccttcg ctcattagct tttgcagagc aaagttagtg gcgtacattt tggtattgga
    20521 agccaggtca tacagcgtcc cggtggtggc tttgacaggc tgttccatca atacgctgcc
    20581 atcgtacttt ttcgccgctc cccaggcttt gcgatacaca atctgattat cttttataat
    20641 cagcaggttt acgccgggat aaccggcgtc cacttgctgg ctaatccagc gatccatctg
    20701 gttaagccgt tcgacgttga accctgcttt ctctggcgaa ctttctgtca gaacagggta
    20761 ttttgcggcg ctgacgctac aactaacagc cagcagagaa agatagagca ttgtccgttt
    20821 catgatatca gtccagattg acgttacggc agccaaagag cgtggtgaaa ataaacccgc
    20881 aaacccatgc caccagaatc ccgccagcat aaaccgccat tgccggaagg atgccttgtg
    20941 aggaagtcat cagcggcagc gccaccagac cagacggccc aaaggcgctg tttaagccca
    21001 tcggcagacc ccaccaggct atcaatccga taaacaaacc gcccgccgcg ccgcctaaac
    21061 aggcggtaat aaacggtttc atgcggggca gggtgacgcc ataaatcagc ggttcgccaa
    21121 cgccaagcag accgggaata atcgccccgc gtacctgact gcgtaatgca ctgtgcggtt
    21181 gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc cgcgcctgcc attgaaagga
    21241 tgggaaataa gctgttgaat ccctggctgt ccattaacgc gaggtaaaca ggaataaagc
    21301 cctgatgcac gccaaatacc acggcgatca ggaacagccc ggctaaaacc gcacaaccga
    21361 gcggattact gttcaggtgc ataaacagcc acgacatacc ttcgaataac cagccgccca
    21421 gcggcataat gatcaggtag gccagcgtag cggtgatcag tagggtgatt aacgaggtca
    21481 gcagcatgtc gagatcgtcc ggcataaagc ggcgtaccat gccttcaatg cgagcgcagg
    21541 cccacgcggc aatcaacacg ccgataatat tgccgcgcgg atcgatgggc agaccaaaga
    21601 aatcgtgaaa accggcgtag taaccggtgg ttgcagcggg gttataaccg agcaaaaaga
    21661 gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa tgcctgggcg gcgttatagc
    21721 ccaccagaat caccaggaag gtgaacaaac ctttgctgaa caccttcata aaattcagcg
    21781 cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat caccgtggca attaacgtcg
    21841 ctatccccag cagcagaccg gcggcaataa aaccggggat aagcggcgtg aagatggtgg
    21901 cgaatttggc gagaaattgt tgcacgccgg aggtttgttt ggcttttaac tgacgtttgt
    21961 tctgggcggc gatttctgcg gcatcctgta ccggtgcttc tcccagcagc tcgctcatcg
    22021 cttttgcggc acgatgcgct ttaccaggcc caaaaaccac ctgcacctga tcgctggtca
    22081 aaatgacgcc cttcacgcct tccagcgttt tgatatcggg atcaaccagt gaactgtcat
    22141 gtacacccag acgcaggcgc gtcatacagt taccacaact ggcgatattt cccggtccgc
    22201 cgacacgggt aagaatggtg ttcagaagtt cactgctgat ctctttggcc atgagttatt
    22261 ccttgtctaa aaccttgcga ataaagccgc cgtgttgatc caggcgtttt ttagcttctg
    22321 cggcatcgag atttttcagc accatcacaa tggccgtttt acagttgcgc tcgcaggcaa
    22381 ttaacgccgc ttccgcttgc tctgcgctac atccggtagc gtttttgacg atattgacct
    22441 ggcgcacgtg cagtttttcg ttggtggcga ccacatcgac catcaggttg ccgaacactt
    22501 tgccggattt aatcatcagc ccggtggaga gcatattgag caccagtttc tgcgctgtgc
    22561 ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc tacaatcggt gtaatggcaa
    22621 actcggcggt ggttgaaacg gcactccccg gattacagga aattcccact gtacggcagc
    22681 cgagctggcg tgcatattcc agtccggcaa tcacatacgg cgtgcgaccg ctggcagcaa
    22741 tgccaaccac cacatcctgc gccgttaaat taatattttt cagatcgtta atgccgcctt
    22801 cccggctatc ttccgcgcct tccaccgcgt gctgaatggc atattcgccg ccagcaatca
    22861 aacccacgac cagacccggt ttcacgccgt aggtcggcgg acattcgctg gcatccagaa
    22921 tccccagacg accggatgtt cccgcaccga gataaatcag acgaccgccg ccgctgacct
    22981 gggcgtgaat aacatcgatc gccgcggcga tatccggcag tacgcgctca acggcgagtg
    23041 gtacggtttt atcttcatcg ttgataatcc ggcacatttc cagcgtcgat acgcggtcaa
    23101 tttcagccga agcggcgttc gagccttcgg taatcatctt ttcaagttgc atcggtggtg
    23161 accttactat ttacgatcaa ataattgcat atgtatggaa tcatatatcc cgtattgacc
    23221 gacttaaagg aatcattgag tctacactcg cagagtgtga agagcgtcat aagaacttaa
    23281 gcggaatttt tgctgccagc ccaggagagt ggaagatgtt gtacctgacg aaaatacgca
    23341 atgcggaaag tgaatttacc gggaacgagc aaaaaattgc tgactttctg cgggccaacg
    23401 tgagtgagct gaaatccgtc tcttcgcgca agatggcgaa acagcttggc attagccagt
    23461 cgagtattgt gaagtttgcc cagaagctcg gcgcgcaagg gtttactgaa ttgcgtatgg
    23521 cgttaattgg cgaatacagc gccagccggg agaaaacgaa cgctacggcg ctgcacctgc
    23581 acagttcgat taccagtgac gattcgctgg aagtgattgc ccgtaaactg aatcgcgaaa
    23641 agcagctggc gctggagcaa acctgcgcgt tgtttgactt cgcgcggctg caaaaaatca
    23701 ttgaggtcat cagtaaagca ccgtttatcc agataacggg cctgggcggg tcggcgctgg
    23761 tggggcgcga tctgtcgttc aaactgatga aaattggcta tcgcgtggcc tgcgaagccg
    23821 acacacatgt acaagccacc gtttcccagg cgctgaaaaa aggcgatgta caaatcgcta
    23881 tctcttacag cggcagtaaa aaagagattg tcttgtgcgc cgaggcagca cgaaagcagg
    23941 gggcaacggt gattgccatt acctcgttaa ccgattcccc gctgcggcgg ctggcgcatt
    24001 acaccctcga taccgtttct ggcgaaaccg aatggcgtag ctcgtcgatg tctacccgca
    24061 cggcgcaaaa ttctgttacc gatttactgt ttgttggcct tgtgcaactc aatgatgtcg
    24121 agtcattgaa aatgattcag cgcagcagtg agctaacaca acgcctgaaa tgatgcataa
    24181 agcagcgact ggattgagat tttcctgaat tagtgagctg aaccgcagca atattttgtt
    24241 tatcctgtat tttcagaggg aatggagtgt aacgctctgt attaacaagg agagcattaa
    24301 aatgggtaaa ctcacgggca agacagcact gattacgggc gcattgcagg gaattggcga
    24361 aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta atcttgctgg atatctcccc
    24421 tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt catcgctgta cggcggttgt
    24481 cgccgatgtg cgtgacccgg tgtcggtagc cgcagctatc aaacgcgcga aggaaaaaga
    24541 agggcgcatt gatatcctgg tgaataacgc aggcgtttgt cgtctgggca gtttcctcga
    24601 tatgagcgat gaagatcgcg atttccatat tgatatcaat attaaaggcg tatggaacgt
    24661 cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat ggtcgcattg tgatgatgtc
    24721 ttcggtcact ggcgatatgg tggccgatcc gggtgaaacg gcgtatgcct taacgaaagc
    24781 ggcgattgtt ggcctgacta aatcgctggc ggtggagtat gcgcagtctg gtattcgcgt
    24841 taacgccata tgccctggat acgttcgcac accaatggcg gaaagcattg cccgccagtc
    24901 gaacccggaa gatccagaat cggtgctgac tgaaatggcg aaagcaatcc cgatgcgtcg
    24961 cctcgccgat ccgttggaag ttggcgaact ggcggccttc ctcgcatcgg atgaatccag
    25021 ctatttaacc ggtacacaga atgtgattga tggcggcagc acactgccgg agacggttag
    25081 cgtcggtatc tgattcacct ctgtttcctc cctgcatttg tggggaggat ttcgtcttga
    25141 actacgttca ccaggctatt ttatttgtca ttttggcccc gggcagtgct cgaaatcctc
    25201 acgtactatg tgtacgctcc ggtttctccg cgctgttcgt gtccaaactg actgcaacaa
    25261 ttacgcctgt tgaaccaagt tcttattccc ttttcaactt ccaaatcacc aaacggtata
    25321 taaaaccgtt actcctttca cgtccgttat aaatatgatg gctattagaa agtcattaaa
    25381 tatataaggg tgcgcaatgg ccgttaactt actgaaaaag aactcactcg cgctggtcgc
    25441 ttctctgctg ctggcgggcc atgtacaggc aacggaactg ctgaacagtt cttatgacgt
    25501 ctcccgcgag ctgtttgccg ccctgaaccc gccgtttgag caacaatggg ccaaagataa
    25561 cggcggcgat aaactaacga taaaacaatc tcatgccggg tcatcaaaac aggcgctggc
    25621 gattttgcag ggcttaaaag ccgacgttgt cacttataac caggtgaccg acgtacaaat
    25681 cctgcatgac aaaggcaagc tgatcccggc ggactggcaa acgcgcctgc cgaataatag
    25741 ctcgccgttc tactccacaa tgggcttcct ggtgcgtaag ggcaacccga agaatatcca
    25801 cgactggaac gacctggtgc gctccgatgt gaagctgatt ttcccgaacc cgaaaacgtc
    25861 gggtaacgcg cgttacacct atttagccgc gtggggcgca gcggacaaag ccgacggtgg
    25921 cgataaagcc aaaaccgaac agtttatgac tcagttcctg aaaaacgttg aagtgttcga
    25981 taccggcggt cgtggcgcga ccaccacttt tgccgagcgc ggcctgggtg atgtgctgat
    26041 cagcttcgag tcggaagtga acaacatccg taaacagtat gaagcgcagg gttttgaagt
    26101 ggtgattccg aaaaccaaca ttctggcgga attcccggtg gcatgggttg ataaaaacgt
    26161 gcaggccaac ggtacggaaa aagccgcaaa agcctacctg aactggctct acagcccgca
    26221 ggcgcaaacc atcatcaccg actattacta ccgcgtgaat aacccggaag tcatggacaa
    26281 actgaaagac aaattcccgc agaccgagtt gttccgcgtg gaagacaagt ttggctcctg
    26341 gccggaagtg atgaaaaccc acttcaccag cggcggcgag ttagacaagc tgttagcggc
    26401 ggggcgtaac tgatgtttgc tgtctcttcc agacgtgtgc tgccgggctt taccttaagc
    26461 ctcggcacca gtctgctgtt tgtgtgcctg attttgctgc tgccgctctc cgcgctggtg
    26521 atgcaactgg ctcagatgag ctgggcgcag tactgggagg tgatcaccaa cccgcaggtg
    26581 gtcgcggcct acaaagtgac gctgctgtca gcgtttgtgg catcgatttt taacggcgtt
    26641 tttggcctgc tgatggcgtg gattttaacc cgttatcgct tccctggccg cacgctgctt
    26701 gatgcgctga tggatttacc ctttgcgctg ccaacggcag tcgctggttt aacgctggcc
    26761 tcgctctttt ccgtaaacgg tttttacggt gaatggctgg cgaagtttga tatcaaagtc
    26821 acctacacct ggcttgggat tgcggtggcg atggccttta ccagcattcc gtttgtggtg
    26881 cgtaccgtgc agccggtgct ggaagagtta ggcccggaat atgaagaagc ggcggaaacg
    26941 ctcggtgcca cgcgctggca gagtttctgc aaagtggtgt taccggagct ttctccggcg
    27001 ctggtggcgg gtgtggcgct gtcgtttacc cgcagcctcg gtgagtttgg cgcggtgatt
    27061 tttatcgccg ggaatatcgc ctggaagaca gaagtgacgt cgctgatgat ctttgtgcgc
    27121 ttgcaggagt ttgattaccc ggcggcgagc gcgattgctt cggtgatcct cgcggcatcc
    27181 ctgctgctgc tgttctcaat taacactctg caaagtcgct ttggtcggcg tgtggtaggt
    27241 cattaatggc ggaagtgaca cagttgaagc gttatgacgc gcgcccgatt aactggggca
    27301 aatggtttct gattggcatc gggatgctgg tttcggcgtt catcttgctg gtgccgatga
    27361 tttacatctt cgtgcaggca ttcagcaagg ggctgatgcc ggttttacag aatctggccg
    27421 atccggacat gctgcacgcc atctggctga cggtgatgat tgcgttgatt gccgtgccgg
    27481 taaatctggt gttcggcatt ctgctggcct ggctggtgac gcgctttaac ttccccggac
    27541 gccagttact gctgacgcta ctggacattc cttttgccgt gtcaccggtg gttgccggtc
    27601 tggtgtatct gctgttctac ggctccaacg gcccgctcgg cggctggttg gatgaacata
    27661 atctgcaaat tatgttctcc tggcccggaa tggtgctggt cactatcttc gtgacgtgtc
    27721 cgtttgtggt gcgcgaactg gtgccggtga tgttaagcca gggcagtcag gaagacgaag
    27781 cggcgatttt gcttggcgca tccggctggc agatgttccg ccgtgtcaca ttgccgaaca
    27841 tccgctgggc gctgctttat ggcgtagtgc tgaccaacgc ccgcgcgatt ggcgagtttg
    27901 gcgcggtgtc ggtggtttcc ggctcgattc gtggcgaaac actttcgctg ccgttacaga
    27961 ttgaattgct ggagcaggac tacaacacag tcggctcctt taccgccgcc gcgctgttga
    28021 cgctgatggc gattatcacc ctgtttttaa agagtatgtt gcagtggcgc ctggagaatc
    28081 aggaaaaacg cgcgcagcag gaggaaaatc atgagcattg agattgccaa tattaagaag
    28141 tcgtttggtc gcacccaggt gctgaacgat atctcactgg atattccttc aggtcagatg
    28201 gtcgcgttgc tggggccgtc cggttccggg aaaaccacgc tgctgcgcat tatcgctggg
    28261 ctggagcatc aaaccagcgg gcatattcgc ttccacggca ccgacgtgag ccgcctgcac
    28321 gcacgtgatc gtaaagtcgg tttcgtgttc cagcattacg cgctgttccg ccatatgacg
    28381 gtgttcgaca atatcgcttt tggcctgacg gtgctgccgc gtcgcgagcg cccgaatgcc
    28441 gcggcgatca aagcgaaagt gacaaaattg ctggagatgg tgcagcttgc tcatctggcg
    28501 gatcgttatc cggcgcagct ttccggcggc cagaaacagc gtgtggcgct ggcgcgtgcg
    28561 ctggctgtgg aaccgcaaat tctgctgctt gatgaaccgt ttggcgcgct ggatgcgcag
    28621 gtgcgtaaag agctgcgtcg ctggctgcgt caactccatg aagaactaaa attcaccagc
    28681 gtttttgtga cccacgatca ggaagaagcg accgaagtag ctgatcgtgt agttgtgatg
    28741 agccagggca atattgaaca ggctgacgcg ccggatcagg tatggcgcga accggcgacc
    28801 cgttttgtgc tcgaatttat gggcgaagtg aaccgcctgc agggaaccat tcgcggcggg
    28861 cagttccatg ttggcgcgca tcgctggccg ctgggctaca cacctgcgta tcaggggccg
    28921 gtggatctct tcctgcgccc ttgggaagtg gatatcagcc gccgtaccag cctcgattcg
    28981 ccgctgccgg tacaggtact ggaagccagc ccgaaaggtc actacaccca attagtggtg
    29041 cagccgctgg ggtggtacaa cgaaccgctg acggtcgtga tgcatggcga caatgccccg
    29101 cagcgtggcg agcgtttatt cgttggtctg caacatgcgc ggctgtataa cggcgacgag
    29161 cgtatcgaaa cccgagatga ggaacttgct ctcgcacaaa gcgcctgata ggttgagtga
    29221 atgttaaacg cccggaggcg cttcccgcga tccgggcttt ttaatggtgt aggcctgata
    29281 agacgcgcaa gcgtcgcatc aggcaacacc acgtatggat agagatcgtg agtacattag
    29341 aacaaacaat aggcaatacg cctctggtga agttgcagcg aatggggccg aataacggca
    29401 gtgaagtgtg gttaaaactg gaaggcaata acccggcagg ttcggtaaaa gatcgtgcgg
    29461 cgctttcgat gatcgtcgag gcggaaaagc gcggggaaat taaaccgggt gatgtgttaa
    29521 tcgaagccac cagtggtaac accggcattg cgctggcaat gattgccgcg cttaaaggct
    29581 atcgcatgaa attgctgatg cccgacaaca tgagccagga acgccgtgcg gcgatgcgtg
    29641 cttatggtgc ggaactgatt ctggtcacca aagagcaggg catggaaggt gcgcgcgatc
    29701 tggcgctgga gatggcggat cgtggtgaag gaaagctgct cgatcagttc aataatcccg
    29761 ataaccctta cgcccattac accactaccg ggccggaaat ctggcagcag actggcgggc
    29821 gcattaccca ttttgtctcc agcatgggaa caaccggcac tatcaccggc gtgtcacgct
    29881 ttatgcgcga acaatccaaa ccggtgacca ttgtcggcct gcaaccggaa gagggcagca
    29941 gcattcccgg cattcgccgc tggcctacgg aatatctgcc ggggattttc aacgcttctc
    30001 tggtggatga ggtgctggat attcatcagc gcgatgcgga aaacaccatg cgcgaactgg
    30061 cggtgcggga aggaatattc tgtggcgtca gctccggcgg cgcggttgcc ggagcactgc
    30121 gggtggcaaa agctaaccct ggtgcggtgg tggtagcgat catctgcgat cgtggcgatc
    30181 gctacctttc caccggggtg tttggggaag agcattttag tcagagggcg gggatttaag
    30241 attattgcgc aggcactgtt ttatccgtct gcgcaattaa cgcatgtaaa aaaggctgtg
    30301 cgttctcatc gctcttaccg gggatcttca caatatacgg cttcccggta atcgatgacg
    30361 acgaagcaac cttatcaata aactgctcgg cagtgtcaat gcggttacgg gtattgccca
    30421 gcttcagacg cagatgagaa accgcttcat cgcaggtatg ttcatcaccg ttgcgcacaa
    30481 atatcaaatc ctttttctgt gctaatccct ccagcatggc gttgatgcgg gcttcttcat
    30541 gggcagttaa cttcgcgtaa gcggggagtg tcattagtag tgtaatgacc agacaaatga
    30601 ttttcttcac tgaattacct tatccttgtt ttgtaatagc cgatttgact gccagaatca
    30661 ggtaaagttt cggttggaat acgatgagga tcatcggcaa ggagaaaaaa ttaaatgagt
    30721 agtttgttgt tgtttaacga taagagcagg gcactgcagg cggatatcgt cgccgtgcag
    30781 tcgcaggtag tgtacggcag cgtaggcaac agcattgccg tgcctgctat caaacagaac
    30841 ggcctgaatg tctttgccgt gccgacggta ttgctgagca atacgccgca ttatgacact
    30901 ttctacggtg gtgcgattcc ggacgaatgg tttagcggct atttgcgtgc gcttcaggag
    30961 cgtgatgcgc tgcgccaact tcgagctgta accacgggct atatgggaac ggcatcgcaa
    31021 atcaaaatcc ttgccgagtg gctgactgcg ctacgcaaag accatcctga cctattgatc
    31081 atggtcgatc cggtgattgg cgatattgat agcggaattt atgtcaaacc tgaccttccc
    31141 gaggcgtatc gacaatattt actgccgctg gcgcagggaa ttacccccaa tatctttgag
    31201 ctggaaatcc tgaccggtaa aaattgccgc gatctcgaca gtgccattgc tgccgcaaaa
    31261 agtctgcttt cagacacact aaaatgggtg gtgattacca gcgcctccgg taatgaagaa
    31321 aatcaggaga tgcaggttgt ggtggtcact gccgacagcg tgaatgtcat ttcccattca
    31381 cgggtaaaaa ccgacctgaa agggactggc gacctgtttt gtgctcagct catcagtggc
    31441 ttgctgaaag ggaaggcgtt aaccgacgcg gtgcaccgcg cgggtttgcg tgtactggaa
    31501 gtgatgcgct acacccagca acatgagagc gatgaattga ttttgccacc gctggtggaa
    31561 gcataaaaaa atggcgccaa tcggcgccat ttttcactgc ggcaagaatt acttcttgat
    31621 gcggataacc ggggtttcac ccacggttac gctaccggac agtttgatca gttctttgat
    31681 ttcgtccatg ttggagataa caaccggagt cagggtagac ttggctttct cttccagcag
    31741 cggcagatca aattcaatga cagtatcgcc aactttcacg cgctgacctt cttcagcaat
    31801 acgcttgaag ccttcgcctt tcagttcaac ggtgtcgata ccgaagtgga cgaacagttc
    31861 aacgccgcta tcagattcga tagagaatgc gtggttggtt tcaaagattt taccaatggt
    31921 gccgtctaca ggcgcgacca ttttgttacc cgttggtttg atagcaatac catcaccaac
    31981 gattttttcc gcaaaaacga catccggcac gtcttcgata ttgacgatct cgccagagag
    32041 cggagcaatg atctcaatag ttccggtatc cttcttgtcg tcggaaacca gagatttcag
    32101 tttatcgaac aaacccatga tcttctccta agcagtaaat tgggccgcat ctcgtggatt
    32161 agcagattgt tttttcttca atgaacttgt taaccagcgt cattaactcg tccgttgtcg
    32221 gttgagcaag agcctgctct gctaacacct tcgcatcttc gaagttcgtg ttacggataa
    32281 tcttcttaat gcgcgggata gaaatggcgc tcatagagaa ttcgtccaga cccatcccca
    32341 gcaacagaag tgtagcacgt tcatcgccag caagctcacc acacatgcca gtccatttgc
    32401 cttcagcatg agaagcatca ataacttgct tgatcaagtt cagcacggac ggtgacattg
    32461 gctggtaaag gtgtgaaatc atatcattac cacggtcaac tgccagagtg tactgcgtta
    32521 aatcattggt gccgatacta aagaaatcaa cttctttggc taaatgacgt gcaattgttg
    32581 cggcagccgg tgtttccacc attacgccga tttcaattga ctcgtcaaac gctttacctt
    32641 cgtcgcgcag ttcctgtttg tagatttcga tctctttgcg cagtgcacgc acttcttcaa
    32701 cagagatgat catcgggaac ataatgcgca atttaccgaa agccgaggca cgcaggatag
    32761 cgcggagctg atcgcgcagg atctctttac gatccatcgc gatacggata gcgcgccagc
    32821 cgaggaacgg gttctcttct ttcgggaagt tcatgtatgg cagctctttg tcgccgccga
    32881 tgtccatggt acgaacgata accgcctgcg agccacacgc ttcagccact gctttgtaag
    32941 cagcaaactg ttcttcttca gtgggcagcg cgtcgcggtc catgaacagg aactcagtac
    33001 gatacagacc aacgccttca gcgccgttac gctctgcacc ttcaacgtca cgaaccgtac
    33061 caatgttagc gcatacttct acctggtgac cgtccagcgt aatagctggc agatctttca
    33121 gtttagcaag ctctgctttt tcagaagcca cttgctcctg aacagcgcgc attttatcaa
    33181 taacttcgtt ggttggattg acgtaaacct gattatttac ggcatccaga atcagatagt
    33241 cgtcattttt cacctgagag gtgacgctac cggtacccac gatagcaggc agttccagag
    33301 aacgcgccat gatagaggtg tgggaagtac ggccacccgc gtcggtgatg aaacccagca
    33361 ccttcttcag gttcagctgt gcggtttcgg acggcgtcag gtcagcggca accagaatga
    33421 cttcatcctg aatggcgctc aggtcgataa tcttcaggcc caggatgttg cgcagcaggc
    33481 gcttaccgat atcacgtacg tcagccgcac gttctttcag gtattcatca tccagctctt
    33541 ccagggcaga agcctgacct tcgataactt catgagcagc tgcgtcagct gtcatgtgct
    33601 tatctttaat cagggctatg atttcctgct ccagctcctc atcttcgagc agcataatat
    33661 gcccttcaaa gatggcttct ttttcttcac cgaacgtttc accagctttc gttttgatcg
    33721 tttccagctg ggctgatgcc ttggcacgac cgctcagaaa acgttcaact tcctgatcaa
    33781 cctggtcggc agaaattttt ttccggtcaa tgacaatttc gtcttctttc agaagcagag
    33841 ctttaccgaa agcgataccc ggggatgcta aaatgcctga aatcataacc ctaccttact
    33901 tgtgactgat ttttaaaaga acccgggaaa ttactcgagt tccgccatca gtttaaccag
    33961 atgttcaacc gctttctgct cgtcttcgcc ttctgcggag atagtcacaa cggtaccttg
    34021 agtcaggccc agagtctgca gtttaaacag gcttttcgcg ctggcgcttt tgccgttgga
    34081 agtcacagta atttcagaag tgaagccctt agcttctttt acaaactggg cagcagggcg
    34141 ggtgtgcaga ccgttcggag cggtaatggt aacttcttgc tggaacattg tatttcccca
    34201 acttataggt ttagtgttgt ggaactaaag tctagcctgg cggttcgact ttagcctgta
    34261 ttgttagcgc cagtttttaa cagacgcgac gcacgaagag cgcaatacag tccattgctg
    34321 gcgggtgttg accgctgacg tttcgtacgt cattaaacat tatgccgcta aaggaagaat
    34381 tgaaccaaat cataaaatcg attcagcttt ggcttttcct gtaacgatta atttcgcgca
    34441 tcaaaataat tagtctggtt aaataccagt tccgacaggc tgaatcaatg ccaggagggg
    34501 cgaaagtttg aagcaggcca caaaaaagca cctaaaaagg tgctttttta cgcattttta
    34561 acaagctggc attactgttg caattctttc tcagtgaaga gatcggcaaa caatgcggtg
    34621 cttaaataac gctcacccga tgacggtaga ataaccacaa tattcttgtt ggtaaagctt
    34681 tcatcttctt gtagtttcaa cgccgcggca acagctgctc cagaagagat acctgcaaga
    34741 ataccttctt cttccatcag acgacgggcg gtagaaatcg cttcttcatt ggtgatgcca
    34801 atgactttat cgaccagctt gagatcgagg ttagccggga taaaaccagc gccaataccc
    34861 tgaattttat gcgggccagg tttaatctct tcacctgcca gcgcctgggc gataactgga
    34921 gaatcggttg gctcaacggc gacagagata agatcggtct tacctttggt gcctttaatg
    34981 tagcggctga cgccagtcag cgtaccgcca gtcccaacgc cagcaataaa tatatcaacc
    35041 tgaccgtcgg tatcttccca gatctccgga ccggtggttt tttcgtgaat ttcagggttt
    35101 gccggattgc tgaattgttg cagcagcagg tatttctctg gattgctggc gacaatttct
    35161 tctgcttttt ggattgcgcc tttcatgcct ttagcacctt cggtcagcac caggtttgca
    35221 cctaacgctt tcagcagctt gcggcgttca atactcatgg tttctggcat ggtcagggtg
    35281 agtttgtaac cgcgagcggc agctacatag gccagtgcaa tcccggtatt accgctggtt
    35341 ggttcaacaa gttcaacgcc tggtttcagc acgccgcgct tttcggcatc ccaaatcatg
    35401 ttggcaccga tacggcactt aacgctgaag ctggggttac gagattccac cttcgccaga
    35461 atgcgtccgt taccgatgcg attcaggcga accagcggcg tgtgaccgat agtcagcgag
    35521 ttatcttcaa aaatcttact catggcctgt ccttaactgt atgaaattgg gatacaacag
    35581 gtagcatacc cgctcagaga atatgcggaa gtaaggattt agcatatcta tatgcagaag
    35641 ggaaataatg acatgcaaga tggaataagg ggcggcataa gccacccctg tttcacacaa
    35701 acggtttaca aaataaccgg tagattgtta ccgccacatc gcgtgtttat cgcgatagca
    35761 atcgacccac atcgccgtcg cgccacaaac ggcaacgggc atgatgaaca gattaagcag
    35821 cgggatcatc gtaaacaggc tggttaaagc accaaactgc atattggtga ttttgcgtgt
    35881 gcgcagggcg gtgcgcatct ctttaaacgg cactttgtgg ttatcgaagg ggtaatcgca
    35941 atactggatg gctaacatcc aggcgctaaa caggaaccac agtaccggcg cgacggtttg
    36001 cccaatacca gggatgaagt aaagaattag taatacaatt gcgcgcggca gataccaggc
    36061 aaatttttgc cattcgcgtt tcatgattcg cggcacatct ttcatgatac cgaaaatccc
    36121 ggtatctggc ggtgtagcgc cagtcaatcg tgcttccagt tgttcagcca ataaaccgtt
    36181 aaacggagcg gcaatccagt tagcaatcgt ggagaagaaa tagccaaaca ctaacagcac
    36241 agagatgaca gccagaggcc acaacagata gctcagccat tgtaaccagt ccggaacata
    36301 actcatgaga gtcgggatcc agacatcgag ttgcgtaaag agccaccaga atgcgccccc
    36361 catcaacaaa atattgacca gcagcggtaa aataacgaaa cgccgaatcc caggttgcga
    36421 gacgagcttc cagccttgcg caaaatagta aaaaccgctg cgtggggcag atgtgaatga
    36481 tgaaaccata atcaggatga gctccttttg accaatccca ggaaaattgt gcgtatttta
    36541 ccgggtaatt gcgcaatgga cagttaggat atgttcgaaa aaacagcaaa aagcacgatt
    36601 tcatctatct ttgtgctgtg aaagttaata gtgcacttgc acttgaggta atcggcaaat
    36661 actcttagtg agtaaatgtt tgccgtggtg gcaaggtgtt agaacaacag agaatataat
    36721 gatgcaggat ttgcgtctga tattaatcat tgttggcgcg atcgccataa tcgctttact
    36781 ggtacatggt ttctggacca gccgtaaaga acgatcttct atgttccgcg atcggccatt
    36841 aaaacgaatg aagtcaaaac gtgacgacga ttcttatgac gaggatgtcg aagatgatga
    36901 gggcgttggt gaggttcgtg ttcaccgcgt gaatcatgcc ccggctaacg cccaggagca
    36961 tgaggctgct cgtccgtcgc cgcaacacca gtaccaaccg ccttatgcgt ctgcgcagcc
    37021 gcgtcaaccg gtccagcagc cgcctgaagc gcaggtaccg ccgcaacatg ctccgcgtcc
    37081 agcgcagccg gtgcagcaac ccgtgcagca gcctgcctat cagccgcagc ctgaacagcc
    37141 gttgcagcag ccagtttcgc cacaggtcgc gccagcgccg cagccagtgc attcagcacc
    37201 gcaaccggca caacaggctt tccagcctgc agaacccgta gcggcaccac agcctgagcc
    37261 tgtagcggaa ccggctccag ttatggataa accgaagcgc aaagaagcgg tgattatcat
    37321 gaacgtcgcg gcgcatcacg gtagcgagct aaacggtgaa ctgcttctta acagcattca
    37381 acaagcgggc ttcatttttg gcgatatgaa tatttaccat cgtcatctta gcccggatgg
    37441 cagcggcccg gcgttattta gcctggcgaa tatggtgaaa ccgggaacct ttgatcctga
    37501 aatgaaggat ttcactactc cgggtgtcac tatctttatg caggtaccgt cttacggtga
    37561 cgagctgcag aacttcaagc tgatgctgca atctgcgcag catattgccg atgaagtggg
    37621 cggtgtcgtg cttgacgatc agcgccgtat gatgactccg cagaaattgc gtgagtacca
    37681 ggacatcatc cgcgaagtta aagacgccaa cgcctgatac acttaaggca aattaactcc
    37741 tcttcgaacc cccgcttgtc gggggttttt agcattgatg gtgcgatatg gaatcaatcg
    37801 aacaacaact gacagaactg cgaacgacgc ttcgccatca tgaatatctt tatcatgtga
    37861 tggatgcgcc ggaaattccc gacgctgaat acgacaggct gatgcgcgaa ctgcgcgagc
    37921 tggaaaccaa acatccagaa ctgattacgc ctgattcgcc tacccaacgt gtaggtgctg
    37981 cgccgctggc ggctttcagc cagattcgcc atgaagtgcc aatgctgtcg ctggataacg
    38041 tttttgatga agaaagcttt cttgccttca acaaacgagt gcaggaccgt ctgaaaagca
    38101 acgagaaagt cacctggtgc tgtgagctga agctggatgg ccttgctgtc agtattctgt
    38161 atgaaaatgg cgttttagtc agtgccgcga cccgtggcga tggcaccacc ggggaagata
    38221 tcacgtctaa cgtgcgtacc attcgcgcca ttccactgaa gctgcacgga gagaatatcc
    38281 cggcgcgtct ggaagtgcgt ggtgaagtgt tcctgccgca ggcggggttc gaaaagatta
    38341 acgaagatgc gcgacgcacg ggcgggaaag tttttgctaa cccacgtaat gcggcagctg
    38401 gttcactgcg tcagcttgat ccgcgtatta cagcgaagcg accgctcact tttttctgct
    38461 atggcgttgg tgttctggaa ggtggcgagc tgccggatac tcatcttggc cgtttactgc
    38521 aatttaaaaa gtgggggttg ccggtcagcg atcgggtaac gctttgtgaa tcggcggaag
    38581 aagtgctggc gttctatcac aaagtggaag aagaccgccc gacgctgggc tttgatatcg
    38641 acggcgtggt gattaaggtc aactcactgg cacagcagga gcagcttggc tttgtcgcgc
    38701 gagccccgcg ctgggcggta gcgtttaaat tcccggcgca ggaacagatg acctttgtgc
    38761 gtgacgtcga gtttcaggtt gggcgtactg gcgcgattac gcctgttgcg cgtctggaac
    38821 ctgtccatgt tgcaggcgtg ctggtgagta acgcaacctt acacaatgcg gatgaaatcg
    38881 aacgtcttgg tttacgcatt ggcgataaag tggtgattcg ccgcgctggc gacgtgatcc
    38941 cgcaggtggt gaacgtcgtg ctttctgaac gcccggaaga tactcgtgag gttgtattcc
    39001 cgacgcattg tccggtatgt ggttctgacg ttgagcgtgt ggaaggtgaa gcggttgccc
    39061 gctgtaccgg tggcctgatt tgcggtgcgc agcgtaaaga gtcgctgaaa cactttgttt
    39121 cccgccgtgc gatggatgtt gacggaatgg gcgacaaaat catcgatcag ctggttgaaa
    39181 aagaatatgt ccacactccg gcggatctgt tcaaactcac cgcaggcaaa cttaccggac
    39241 tggagcgtat ggggccaaaa tcggcacaaa acgtggttaa cgcgctggaa aaagcgaaag
    39301 aaaccacctt tgctcgcttc ctctatgcac ttggcatccg tgaagtcggc gaggccaccg
    39361 cagcaggtct ggcggcatat ttcggcacgc tggaagcgct ggaagccgct tcgattgaag
    39421 agctgcaaaa ggtgcctgat gttggcattg tcgttgcatc ccacgttcac aacttctttg
    39481 ccgaagaaag caaccgcaat gtcatcagcg agctgttggc ggaaggtgtt cactggcctg
    39541 cgccgatcgt tatcaacgcg gaagagattg acagcccgtt tgctggtaaa accgtggtgc
    39601 ttacgggcag cttaagccag atgtcgcgtg atgacgctaa agctcgactg gtcgaactgg
    39661 gcgcgaaagt cgcgggcagc gtgtcgaaga aaaccgatct ggtgatagcg ggtgaagctg
    39721 caggatctaa actggcgaag gcgcaggaac tgggcattga agtcatcgac gaagcggaaa
    39781 tgctgcgttt gctgggtagc tgagatggaa aaagagcagc tgattgaaat agccaatacg
    39841 ataatgccgt ttggcaaata caaagggcgt cgcttaatcg acctgccgga ggagtatctg
    39901 ctgtggtttg cccgcaaaga tgagttcccg gcagggaagc tcggtgagct aatgcaaatc
    39961 acgctgctga ttaaaaccga ggggctgacg caactggtcc agcccctgaa gcgtccgctt
    40021 taagctttat cggtgctgct ttcctgctgc gcctgtaact gttcggtctg gcgtttgtat
    40081 cgacgcgcca gcaccgcaca gaccatcaat tggatctgat ggaaaatcat caggggcagc
    40141 accatcatac cgatcaccga tgtggggaac agaatgtttg ccatcgggat gccatttgcc
    40201 agactctttt tcgaaccaca aaagacgata gtaatttcat ctgccttatt gaagcccagt
    40261 cggcgtgcca taaagacgtt aactacaatc acgatagcca gaagaacgca gctgaccacc
    40321 acgataaaca gcaatgatcc ccagccaact ttatgccaga taccattaac gacggcttcg
    40381 ctgaacgctg tataaaccac caacagaatg gacgtctggt cagttttcgc aatccatttt
    40441 ttattgcgcg acacccagtc accaatccac ggccgggaaa gatgccccaa cacaaacggc
    40501 agcagcagtt gcagcataat tttaccgacc tgctcaaggc tgccccctgc accgtgaaca
    40561 ttcatcacca gaccaaccag caatggtgaa aggaaaatcc ccagcaggct ggatgctgac
    40621 gcagaacaaa ccgccgccgc gacgttaccg cccgccattg acgtgaaggc gattgcagac
    40681 tgcacggtag ccgggagaat gcacaagtag agaaaaccgg agtagagcat cgggtcgaca
    40741 tttaccggtt tccaccaggc aaacagtaca cccagaatcg gaaacagcac gaaggtgctg
    40801 cacattaccc acaaatgcag tcgccagtga ccaccgccag caataatcgc ctcacgcgac
    40861 aacttcgcgc cgtgcataaa gaacagcagg gcaatagctg cggtggtcag attttcaaag
    40921 aaggggacga aatcgcctct ggccggaaag aaagaggcca gcaacaccac cgtgatcagg
    40981 gttaaggtga aaggatcgag gatacgaaaa agtttcataa agactccaga tgatcgatgg
    41041 ttctattgtg cttcttccag tttgagaaat aaaattgatt tattgcatcc attcatgaag
    41101 aaaacagatg aattattctt taaaacaatt aaaggttttc gtcacagtag cgcaggagaa
    41161 aagttttagt cgtgcaggag agcgtatcgg cctgagccag tcggcagtaa gtcacagtgt
    41221 gaaggagctg gaaaatcata ctggtgttcg cctgctggac agaaccacgc gtgaagtggt
    41281 gcttacagat gcagggcagc agttggcttt gcgtcttgag cgactgctgg atgaacttaa
    41341 cagcacgttg cgcgataccg ggcgtatggg gcaacaactg agcggaaaag ttcgggtcgc
    41401 tgccagccag accatttccg cgcatcttat tccgcaatgc attgctgaaa gccatcgccg
    41461 ctatccagat attcagtttg tcctgcacga tcgtccgcag cagtgggtga tggaaagtat
    41521 tcgccaggga gatgtcgact ttggcatcgt catcgatcca ggcccagtgg gcgatctgca
    41581 atgtgaagcg attctttccg agcctttctt tctgctttgc catcgggata gcgctttggc
    41641 cgcggaagat tatgtaccct ggcaggcatt acagggagcg aaattggtgt tgcaggatta
    41701 cgcgtcaggc agccgaccgc tgattgacgc agcgctggcg cgcaatggta ttcaggcgaa
    41761 tattgtgcag gagattggac atccggcgac gctattcccg atggtagccg caggcatcgg
    41821 catcagtatt cttcccgcac tggcgctacc attgcctgaa ggtagcccgc tggtggtaaa
    41881 gcgcattacg ccggttgttg aacgccagtt gatgctggtg cgcaggaaga accgttcact
    41941 ttccaccgcc gctgaagcgt tatgggacgt ggtacgagac cagggcaatg cgctaatggc
    42001 tgggcgggaa ggggatccgt tgtatcagat atagatatcg atatgagtcg ttttttcaac
    42061 aggatttaga agggatacat tcagagacaa acgctgctct ttttcctgcg ccttttctgc
    42121 ctgctgtctc tgcaactgcg ccagttgtgt ttccagtagg gtgatttgct gttgaatcaa
    42181 ttcggcctgt ttttgtttgt cttcagcact gccgctagtg tctacaatct ctttaatttg
    42241 ttgagtgagt ttgatgattt gctgacttat ccgcgcgatt tgtgaagcaa catcgctacc
    42301 ttgtgatgcc ctgttactac tctgtgtttg tatggaagga attgaagttg aagagatagt
    42361 ggccattgtt cagtcctcgt cactgtacga ggtaatatcg gtagaagaac aaggaacttg
    42421 agaagaaaaa tcaatcttac aaatcgcaga tagcaaaaaa gcgcctttag ggcgcttttt
    42481 tacattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
    42541 taccaactga gctaacgacc cgaagtggtg ggtgatgacg ggatcgaacc gccgaccccc
    42601 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cacttcggta cttcacattt
    42661 ttacaagaaa tttagctggt gagaaagtgg tgggtgatga cgggatcgaa ccgccgaccc
    42721 cctccttgta agggaggtgc tctcccagct gagctaatca cccacttctc aatttcttgc
    42781 tacacggcgg agactacata tagtagttgg tgggtgatga cgggatcgaa ccgccgaccc
    42841 cctccttgta agggaggtgc tctcccagct gagctaatca cccccgctgt gtggagtcgc
    42901 attataggga gagttcaaaa tgagtcaacg cattttctaa agaaattatt cgttcgtcgt
    42961 aaatttaagc aagatgatcg caaaacagac cgtgttgcgc aatttgtcaa cgaaaacaat
    43021 aatgcgtaag gttgcaaccc gaactacatt gaggaatcag gcgggagtga tagaatatcg
    43081 cccactcaat ttttccagga tttgccggtt gtcggcatct ttctaaacgt aaggccattt
    43141 catgaaaatc aaaactcgct tcgcgccaag cccaacaggc tatctgcacg ttggcggcgc
    43201 gcgtactgct ctttactcct ggctttttgc acgtaaccac ggcggtgagt tcgtgctgcg
    43261 tattgaagac accgatcttg agcgttccac gccggaagct atcgaagcca ttatggatgg
    43321 catgaactgg ctgagcctgg agtgggatga aggtccgtac taccagacca aacgttttga
    43381 tcgctacaac gcggtgatcg atcagatgct ggaagagggc acagcttata aatgctattg
    43441 ctctaaagag cgcctggaag cgctgcgcga agagcaaatg gcgaaaggtg agaagccgcg
    43501 ttatgacggt cgctgccgcc acagtcatga gcatcacgct gatgatgagc cgtgtgtcgt
    43561 acgttttgct aacccgcagg aaggttctgt tgtttttgac gatcagatcc gtggtccgat
    43621 cgagttcagc aaccaggaac tggacgatct tattatccgc cgtaccgatg gttccccaac
    43681 ctataacttc tgtgtggttg tcgatgactg ggatatggaa atcacccacg ttatccgtgg
    43741 cgaagaccat atcaacaaca cgccacgcca gatcaacatt cttaaagccc tgaaagcgcc
    43801 ggtaccggtt tacgcgcacg tttctatgat caacggcgat gacggcaaaa aactgtccaa
    43861 acgtcacggg gcagtcagcg taatgcagta tcgtgatgac ggttatttgc cagaagcact
    43921 gctgaactat ctggtgcgtc tgggctggtc ccacggcgat caggaaatct tcactcgtga
    43981 agagatgatc aaatacttca ctttgaatgc cgtcagcaaa tctgccagtg cgttcaacac
    44041 cgacaagctg ctgtggctga accatcacta cattaacgcg ctgccgccgg agtatgttgc
    44101 tactcactta cagtggcaca ttgagcagga aaatatcgat acccgtaacg gcccgcagct
    44161 ggctgatctg gtgaaactgc tgggcgaacg ctgcaagacg ctgaaagaga tggcacagag
    44221 ctgccgttat ttctacgaag attttgctga gttcgatgcc gatgccgcga aaaaacatct
    44281 gcgtccggta gcgcgtcagc cgctggaagt agttcgtgac aaactggccg cgattactga
    44341 ctggacagct gaaaacgttc atcacgctat tcaggcgacg gcggatgagc tggaagtggg
    44401 tatgggtaaa gttggtatgc cgctgcgtgt cgccgtaacc ggtgcggggc agtctccggc
    44461 actggatgtt accgttcacg caatcggtaa gacacgtagc attgagcgta tcaacaaagc
    44521 gctggccttt attgctgaac gcgaaaacca gcagtaatta gcgtgtaaaa agataaacgg
    44581 caggagataa tatcctgccg ttttttattt atgctgattc gtcaatatcg agacgggcga
    44641 ggaaattgcg aattccttca cgtgacaaaa attgcgcgac tttttcctgc tcaatgtctg
    44701 tcatattatc tatcgcgttg atgatttgtc gatacgccgg ggcgcgtgct ccaggagcgt
    44761 attcagcaat tctctcttca gcttccatca acattggtgt gttatggaac gcaggcgtgt
    44821 tctgaataaa ctcgcaaact tgcgtatcga ccagaataag tctggcacga ccgcctttca
    44881 cgccgggaaa tttttccgtt ttccagcctt tttctctgat ccaacggtta acagtttgtt
    44941 tagccacacc gaggcattca gccagttctt cggtggtcat tttattgcgt aatcttttca
    45001 tattatttgc tatcgcgaat ccctaagcgt tgtaacaatc cgataatccc ttcccgcaga
    45061 agcaaggatg taaactgttt ttgttcaact ggcgtcatct ctttcgccag tgtcaccagc
    45121 aatacttcaa gcggcgcatc accagaaagg gcaggcgctt ctccctgacc ttctggacgt
    45181 tcagcattac gaatatattc acgaacctgt tcattgacgt gaaccagtcg ggctttgcca
    45241 ccctggacgc ctggttttgg tgacgtcgtc cagccttcct tgcgtaccca tttattaatg
    45301 gtctggcggc tatagccggt cagtctggca agttcatctg gcgtcatcct ctccttgaac
    45361 ataacaattc cctgaatgta tgtgggtcta ttagtagttc attttatagc accactttac
    45421 aggaaaccgt gacatgaata actactgaat gcgagcggtt ttccaaaatt gttcatttgc
    45481 ctgatttttc agcgattgaa actacacgct gcaatttgcc gttgacacat tcgggcggaa
    45541 ttcatatgat gccgcccgtc aactcgacaa gctttacgtg acggggctat agctcagctg
    45601 ggagagcgct tgcatggcat gcaagaggtc agcggttcga tcccgcttag ctccaccaaa
    45661 ttttgcaccc agcaaatttg gtacgtaaac gcatcgtggg gctatagctc agctgggaga
    45721 gcgcttgcat ggcatgcaag aggtcagcgg ttcgatcccg cttagctcca ccaaatttcc
    45781 aaccctcgct gcaaagcggg ggttttttgt ctctgctttt tgccgctttt gtaatacagt
    45841 ctacgtccgg gttagtgccg cctggtgaaa gcatcattgg atgaaaaatc ggcaacagga
    45901 tggcccctgt ttgcttcgca atgcgaataa acttattatt tgtgtgcctg aaaaccccga
    45961 tcagtgagag tagtgtactc atgtttgtgg agcataacct gataaaaaat atcaagatat
    46021 tcacactagc gtttacgctc accgtggtac ttattcagct atcccgtttt atttcgccac
    46081 ttgccattat ccattccagt tatatctttc tggcgtggat gccactgtgc gtaatgctgt
    46141 caatcttgtt tatctttggc tggcgcggtg tcgttcccgt tttatgcggg atgttttgca
    46201 ccaatctgtg gaactttcat ctctcttttt tacagactgc ggtcatgctc ggcagccaga
    46261 catttgtcgt gttgtgtgcc tgcgcaatat tacgctggca gctggggacg cgttggcgtt
    46321 atggattgac cagccgatat gtctggcaac gtctgttctg gcttggtttg gtgacgccga
    46381 tcggcatcaa atgcagcatg tatcttgtgg ggagcttctt tgatttcccg ctaaagatat
    46441 ccaccttttt cggcgatgcg gatgccattt tcacggtcgt ggatttacta agccttttca
    46501 ccgctgttct gatttacaac atgcttttct actatctcac tcgcatgatt gtaagtcccc
    46561 actttgcgca gatattgtgg cgcagggata tcgctccgtc attgggcaaa gagaaacgcg
    46621 catttacctt aagctggctg gcagcgttaa gcgtgctgct acttctgctg tgcacacctt
    46681 atgaaaacga ctttattgca ggttacctgg tacccgtttt cttcatcatc tttaccctcg
    46741 gggtcggtaa gcttcgctat ccgtttttaa atctcacctg ggctgtttca acgctttgcc
    46801 tgctgaatta caaccagaac tttttgcaag gggtggaaac cgaatattcg ctggcattta
    46861 ttctcgcggt gctgatttcc tttagcgttt gcctgctcta tatggtgcgc atttatcatc
    46921 gcagtgaatg gcttaatcgc cgctggcatt tgcaggcgct gaccgatccg ttaacgctcc
    46981 tgcccaactt tcgtgcgttg gaacaagcgc cggagcaaga ggcgggcaag agtttttgct
    47041 gcctgcgcat tgataatctt gagtttatga gtcgtcatta cgggttgatg atgcgcgttc
    47101 actgtatccg atcaatttac cgtacgctgc tgccattgat gcaggaaaac gaaaagttgt
    47161 atcaattgcc gggtagtgaa ctgctgttag tgctgagcgg gccggaaacg gaagggcgac
    47221 tccagcatat ggttaacatc ctgaatagtc ggcaaattca ctggaacaat accgggctgg
    47281 atatgggcta tggtgctgcc tgggggcgtt ttgatggaaa tcaggaaacc ctgcaaccct
    47341 tgttggggca gttaagctgg ctggcggagc aatcctgcgc acatcatcat gtgctggcgc
    47401 tggatagccg agaggagatg gtttccgggc agaccactaa acaggtgcta ttgctgaata
    47461 ccattcgcac ggcgttagat cagggtgatt tgctgctcta cgcccagcca attcgcaaca
    47521 aagagggtga aggttatgat gagatcctcg cgcgactgaa atatgacggc ggcattatga
    47581 ccccggataa gtttctgccc cttattgctc agtttaatct tagcgcgcgt tttgatttgc
    47641 aagtgctgga atccttgttg aagtggctgg caacacaccc ttgcgacaaa aaaggcccgc
    47701 gcttttcagt taatttaatg ccgctcacgc tgctgcaaaa gaatattgcc gggcggatta
    47761 ttcgtctatt taagcgttat cacatctccc cgcaggcggt cattcttgag atcaccgagg
    47821 agcaggcgtt ttctaacgca gaaagcagca tgtacaacat cgagcagctg cataagtttg
    47881 gtttccggat tgcgattgat gactttggca ccggctatgc caactacgaa cggttaaagc
    47941 gtttgcaggc tgatatcatc aaaattgatg gcgtctttgt gaaagatatt gtcacgaata
    48001 cgctggatgc gatgattgtg agatcaatta ccgatctggc gaaagcgaag tcattgagtg
    48061 tggtcgcgga gtttgtcgag acgcaacagc agcaggcgtt attgcataag ctcggggtgc
    48121 aatatctgca ggggtatttg attggtcgcc cgcagccatt agctgattaa caggcgtaaa
    48181 aaaaccgggg aattatcccc ggctttttta tgtatggtct tacagcacca gtgctgcgat
    48241 tgacgcagac agcacactca ccagggtaga gccgtaaacc agtttcagac cgaagcgaga
    48301 aaccacatta ccttgctctt cattcaggcc tttaactgca cctgcaataa ttccgattga
    48361 agagaagtta gcgaaggaaa ccaggaacac agagatgatg ccttcagcac gcggagagag
    48421 cgtggaagca attttctgca gatccatcat cgcaacgaac tcgttagaaa ccagtttggt
    48481 cgccatgata ctgcccactt gcagtgcttc gctggacgga acacccatca cccatgcaat
    48541 cggatagaag atgtagccca ggatgccctg gaaggagatg ctgtagccaa accagccagt
    48601 cacggtggca aacagtgcgt tcagcgcggc gatcagggcg ataaagccga tcagcatcgc
    48661 agcaacgata atggcaactt tgaaacctgc cagaatgtat tcacccagca tttcgaagaa
    48721 gctctgacct tcgtgcaggt tggacatctg aatgttttct tcactggcat caacacggta
    48781 agggttgatc agcgacagca cgataaaggt gctgaacata ttcagtacca gcgctgcaac
    48841 gacatatttc ggctccagca tggtcatgta tgcaccaacg atggacatag aaacggtgga
    48901 catcgccgtt gctgccatgg tgtacatacg gttacgggag attttgccaa ggatatcttt
    48961 ataggcaata aagttttcag actgacccag aatcagggag ctgacggcgt taaaggattc
    49021 cagtttgccc ataccgttga ctttggagag caggaaacca attgcgcgga taatcaccgg
    49081 caacacgcga atgtgctgga gaataccgat cagtgcagag ataaagacga ttgggcacag
    49141 cactttcagg aagaagaatg ccaggccttg atcattcatg ctaccaaaga cgaagttagt
    49201 cccttcgttg gcaaatccga gcagtttttc gaacatttcg gagaagcctt tcacgaagcc
    49261 taaaccaacg tcggagttca ggaagaacca cgccagtaac acttcgataa caagcagttg
    49321 aataacataa cggatacgaa tttttttgcg gtcgctgctt accagcagtg cgagaatcgc
    49381 aacaacggca agtgccagta caaaatgaag gacgcggtcc atatttgctc caaatatgag
    49441 gcaggttaaa tttccgtgca cattctatgt aacatggtta aggaaaacga gatctgacac
    49501 acactataat gacgtcctgt gacaagcttc aaaaatagtg agctgtcata catttctgct
    49561 atgttgtgta tggaagctga aagttatgta aatgtgctaa cattaaacat aaccataaca
    49621 ttcaagtcat caatcttttc gtagtcataa tcctggtcta tcagagaaat caccacaacc
    49681 catttaaatg aatttgataa tcattctcgt ttggcatagc atgaaacata gcaaaggcta
    49741 tgttttagag gcacaagatg acgaactatc gcgttgagag tagcagcgga cgggcggcgc
    49801 gcaagatgag gctcgcatta atgggacctg cgttcattgc ggcgattggt tatatcgatc
    49861 ccggtaactt tgcgaccaat attcaggcgg gtgccagctt cggctatcag ctactgtggg
    49921 ttgtcgtttg ggccaacctg atggcgatgc tgattcagat cctctctgcc aaactaggga
    49981 ttgccaccgg taaaaatctg gcggagcaga ttcgcgatca ctatccgcgt cccgtagtgt
    50041 ggttctattg ggttcaggca gaaattattg cgatggcaac cgacctggcg gaatttattg
    50101 gtgcggcgat cggttttaaa ctcattcttg gtgtttcgtt gttgcagggc gcggtgctga
    50161 cggggatcgc gactttcctg attttaatgc tgcaacgtcg cgggcaaaaa ccgctggaga
    50221 aagtgattgg cgggttactg ttgtttgttg ccgcggctta cattgtcgag ttgattttct
    50281 cccagcctaa cctggcgcag ctgggtaaag gaatggtgat cccgagttta cctacttcgg
    50341 aggcggtctt cctggcagca ggcgtgttag gggcgacgat tatgccgcat gtgatttatt
    50401 tgcactcttc gctcactcag catttacatg gcggttcgcg tcaacaacgt tattccgcca
    50461 ccaaatggga tgtggctatc gccatgacga ttgccggttt tgtcaatctg gcgatgatgg
    50521 ctacagctgc ggcggcgttc cacttttctg gtcatactgg tgttgccgat cttgatgagg
    50581 cttatctgac gctgcaaccg ctgttaagcc atgctgcggc aacggtcttt ggattaagcc
    50641 tggttgctgc cggactgtc
//
LOCUS       DAZGBC010000015        45164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGBC010000015 DAZGBC010000000
VERSION     DAZGBC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.015"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..570
                     /locus_tag="ACHK1J_000914"
     CDS             <1..570
                     /locus_tag="ACHK1J_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7910230.1"
                     /translation="RIMTSRANKYLLAIYPEYHTRLFPDSKLVNESPDIVKDISHANS
                     IHKIYICGMRSVMGMKRGDIIVIYRTGDKKGPARYRSVASTLCVVESVKNISEFLSED
                     SFVDYCIRFSVFSEDELRKIYKERRYPFIIRFTYNLSLPKRPNRAILIDHVGLNGSRA
                     FRWSHFKLTNEQFLKIIELGKINESFIIH"
     gene            548..925
                     /locus_tag="ACHK1J_000915"
     CDS             548..925
                     /locus_tag="ACHK1J_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO7910231.1"
                     /translation="MKVLLSIKPEFAEKILNGTKRFEFRKGIFKNPQISTVVIYATMP
                     LGKVVGQFRIESILSDEPESLWKKTEKHAGISKQFYDSYYSGREKAYAIKIGEVERYK
                     EPIPISALGSNIKPPQSYLYLPA"
     gene            1406..1612
                     /locus_tag="ACHK1J_000916"
     CDS             1406..1612
                     /locus_tag="ACHK1J_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO7910232.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            1687..1983
                     /locus_tag="ACHK1J_000917"
     CDS             1687..1983
                     /locus_tag="ACHK1J_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7910233.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPLHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1989..2774
                     /gene="bet"
                     /locus_tag="ACHK1J_000918"
     CDS             1989..2774
                     /gene="bet"
                     /locus_tag="ACHK1J_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7910234.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2771..3451
                     /locus_tag="ACHK1J_000919"
     CDS             2771..3451
                     /locus_tag="ACHK1J_000919"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7910235.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVTPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3448..3606
                     /locus_tag="ACHK1J_000920"
     CDS             3448..3606
                     /locus_tag="ACHK1J_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO7910236.1"
                     /translation="MTHPHDNIRVGAITFVYSITKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKQEDI"
     gene            3603..4667
                     /locus_tag="ACHK1J_000921"
     CDS             3603..4667
                     /locus_tag="ACHK1J_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HFO7910237.1"
                     /translation="MSQVGNHSFEFPASQGVQGGTVTLFLTIPGRSLARFLASDNYGH
                     TLARSQREINPNRVRKFLNYLTNADSRNEPFIIPPLVGNCDSNIEFVPFGNTNVGIAR
                     IPLDAEIKLFDGQHRAAGIEIFCRSSPSTLMVPMMLTMNLPLKTRQQFFSDINNNVSK
                     PSATINMAYNGRDDIAQGMISFLTQHTVFADITDFEHNVVPLKSNMWVSFKALTDATS
                     KFARNGNQQLEMGYIESVWEAWITLTQIDSIRHGVHHATYKRDYIQFHGVMINAFGFA
                     VQQMMVNHSIAEITSMIEKLCATTSSAEREDFFLMDNWAGICTKASQEKLSVIANVAA
                     QKAAANRLIQAFTKGSLETT"
     gene            4821..5042
                     /locus_tag="ACHK1J_000922"
     CDS             4821..5042
                     /locus_tag="ACHK1J_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7910238.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(5253..5855)
                     /locus_tag="ACHK1J_000923"
     CDS             complement(5253..5855)
                     /locus_tag="ACHK1J_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910239.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            6098..6442
                     /locus_tag="ACHK1J_000924"
     CDS             6098..6442
                     /locus_tag="ACHK1J_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910240.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            6566..6766
                     /gene="torI"
                     /locus_tag="ACHK1J_000925"
     CDS             6566..6766
                     /gene="torI"
                     /locus_tag="ACHK1J_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO7910241.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            7010..7183
                     /locus_tag="ACHK1J_000926"
                     /pseudo
     CDS             7010..7183
                     /locus_tag="ACHK1J_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7278..8525)
                     /locus_tag="ACHK1J_000927"
     CDS             complement(7278..8525)
                     /locus_tag="ACHK1J_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO7910242.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8597..9511)
                     /locus_tag="ACHK1J_000928"
     CDS             complement(8597..9511)
                     /locus_tag="ACHK1J_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO7910243.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDERGMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9727..11160
                     /gene="cscA"
                     /locus_tag="ACHK1J_000929"
     CDS             9727..11160
                     /gene="cscA"
                     /locus_tag="ACHK1J_000929"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO7910244.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNCKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(11168..12163)
                     /locus_tag="ACHK1J_000930"
     CDS             complement(11168..12163)
                     /locus_tag="ACHK1J_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7910245.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            12394..12612
                     /locus_tag="ACHK1J_000931"
                     /pseudo
     CDS             12394..12612
                     /locus_tag="ACHK1J_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12630..13958
                     /gene="dsdA"
                     /locus_tag="ACHK1J_000932"
     CDS             12630..13958
                     /gene="dsdA"
                     /locus_tag="ACHK1J_000932"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO7910246.1"
                     /translation="MENAKMNSLIAQYPLVKVLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELIAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMT
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(14066..15604)
                     /gene="emrY"
                     /locus_tag="ACHK1J_000933"
     CDS             complement(14066..15604)
                     /gene="emrY"
                     /locus_tag="ACHK1J_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO7910247.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15604..16767)
                     /gene="emrK"
                     /locus_tag="ACHK1J_000934"
     CDS             complement(15604..16767)
                     /gene="emrK"
                     /locus_tag="ACHK1J_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO7910248.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            17183..17797
                     /gene="evgA"
                     /locus_tag="ACHK1J_000935"
     CDS             17183..17797
                     /gene="evgA"
                     /locus_tag="ACHK1J_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO7910249.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            17802..21395
                     /gene="evgS"
                     /locus_tag="ACHK1J_000936"
     CDS             17802..21395
                     /gene="evgS"
                     /locus_tag="ACHK1J_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO7910250.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAEQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADFYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVVHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(21451..22596)
                     /gene="yfdE"
                     /locus_tag="ACHK1J_000937"
     CDS             complement(21451..22596)
                     /gene="yfdE"
                     /locus_tag="ACHK1J_000937"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO7910251.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(22670..23614)
                     /gene="yfdV"
                     /locus_tag="ACHK1J_000938"
     CDS             complement(22670..23614)
                     /gene="yfdV"
                     /locus_tag="ACHK1J_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO7910252.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(23684..25378)
                     /gene="oxc"
                     /locus_tag="ACHK1J_000939"
     CDS             complement(23684..25378)
                     /gene="oxc"
                     /locus_tag="ACHK1J_000939"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO7910253.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(25432..26682)
                     /gene="frc"
                     /locus_tag="ACHK1J_000940"
     CDS             complement(25432..26682)
                     /gene="frc"
                     /locus_tag="ACHK1J_000940"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO7910254.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISIDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSDFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(27194..27808)
                     /gene="yfdX"
                     /locus_tag="ACHK1J_000941"
     CDS             complement(27194..27808)
                     /gene="yfdX"
                     /locus_tag="ACHK1J_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO7910255.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQKTAAAERISE
                     QGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNVNDDQ
                     YIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQYLMP
                     LKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            28104..28379
                     /gene="ypdI"
                     /locus_tag="ACHK1J_000942"
     CDS             28104..28379
                     /gene="ypdI"
                     /locus_tag="ACHK1J_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO7910256.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKASYLIHPVR"
     gene            complement(28456..28698)
                     /gene="yfdY"
                     /locus_tag="ACHK1J_000943"
     CDS             complement(28456..28698)
                     /gene="yfdY"
                     /locus_tag="ACHK1J_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO7910257.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            29051..29971
                     /gene="lpxP"
                     /locus_tag="ACHK1J_000944"
     CDS             29051..29971
                     /gene="lpxP"
                     /locus_tag="ACHK1J_000944"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO7910258.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            30327..30398
                     /gene="ypdK"
                     /locus_tag="ACHK1J_000945"
     CDS             30327..30398
                     /gene="ypdK"
                     /locus_tag="ACHK1J_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO7910259.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(30463..31701)
                     /gene="alaC"
                     /locus_tag="ACHK1J_000946"
     CDS             complement(30463..31701)
                     /gene="alaC"
                     /locus_tag="ACHK1J_000946"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO7910260.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVSGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            32078..33775
                     /gene="ypdA"
                     /locus_tag="ACHK1J_000947"
     CDS             32078..33775
                     /gene="ypdA"
                     /locus_tag="ACHK1J_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO7910261.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            33790..34524
                     /gene="ypdB"
                     /locus_tag="ACHK1J_000948"
     CDS             33790..34524
                     /gene="ypdB"
                     /locus_tag="ACHK1J_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO7910262.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            34537..35394
                     /gene="ypdC"
                     /locus_tag="ACHK1J_000949"
     CDS             34537..35394
                     /gene="ypdC"
                     /locus_tag="ACHK1J_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7910263.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRENVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(35397..37892)
                     /gene="ptsP"
                     /locus_tag="ACHK1J_000950"
     CDS             complement(35397..37892)
                     /gene="ptsP"
                     /locus_tag="ACHK1J_000950"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7910264.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGEIRYLPLL
                     LGLDLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVIGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(37917..38954)
                     /gene="ypdE"
                     /locus_tag="ACHK1J_000951"
     CDS             complement(37917..38954)
                     /gene="ypdE"
                     /locus_tag="ACHK1J_000951"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7910265.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(38954..40039)
                     /gene="ypdF"
                     /locus_tag="ACHK1J_000952"
     CDS             complement(38954..40039)
                     /gene="ypdF"
                     /locus_tag="ACHK1J_000952"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7910266.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIVNEIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSTESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(40054..41301)
                     /gene="fryC"
                     /locus_tag="ACHK1J_000953"
     CDS             complement(40054..41301)
                     /gene="fryC"
                     /locus_tag="ACHK1J_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO7910267.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(41323..41649)
                     /gene="fryB"
                     /locus_tag="ACHK1J_000954"
     CDS             complement(41323..41649)
                     /gene="fryB"
                     /locus_tag="ACHK1J_000954"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO7910268.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(41868..42833)
                     /gene="glk"
                     /locus_tag="ACHK1J_000955"
     CDS             complement(41868..42833)
                     /gene="glk"
                     /locus_tag="ACHK1J_000955"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO7910269.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPIEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            43037..44294
                     /gene="yfeO"
                     /locus_tag="ACHK1J_000956"
                     /pseudo
     CDS             43037..44294
                     /gene="yfeO"
                     /locus_tag="ACHK1J_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
     gene            44409..44735
                     /gene="ypeC"
                     /locus_tag="ACHK1J_000957"
     CDS             44409..44735
                     /gene="ypeC"
                     /locus_tag="ACHK1J_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO7910270.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(44876..>45164)
                     /locus_tag="ACHK1J_000958"
     CDS             complement(44876..>45164)
                     /locus_tag="ACHK1J_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFO7910271.1"
                     /translation="IRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFGIA
                     LALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
BASE COUNT        12316 a        11095 c        10338 g        11415 t
ORIGIN      
        1 cgaataatga catcaagggc caacaaatat ttactggcga tttatcccga atatcacact
       61 agactattcc ctgattcaaa acttgtaaat gaatcaccag atattgtcaa agatatatcc
      121 catgctaaca gcattcataa aatttacata tgtggaatgc gttctgtgat gggaatgaaa
      181 agaggagata tcattgtcat ctatagaacc ggagacaaaa aagggccagc tcgctatcgt
      241 tctgtagcca gtacattatg tgtagttgag agcgtaaaaa atatttctga atttttaagc
      301 gaagatagtt ttgtagacta ttgtattcgt tttagcgtat tttctgaaga tgaactcaga
      361 aaaatctata aagaacgtcg ataccctttc attataagat tcacatacaa tctgtctttg
      421 ccaaagagac ccaatcgtgc tattttaata gatcatgtgg ggctaaatgg ttcgcgtgca
      481 ttccgatgga gtcactttaa actcacaaat gagcagttct taaagatcat cgagttaggc
      541 aagataaatg aaagttttat tatccattaa gcctgagttt gcagaaaaaa tattgaacgg
      601 aacaaagcgg ttcgagtttc gtaaaggtat attcaaaaat ccgcaaatta gcaccgttgt
      661 tatttatgcc acgatgccat taggtaaagt tgttggtcaa ttccgtattg aatcaatact
      721 aagtgacgaa ccggaatctc tttggaaaaa gacggaaaaa cacgcaggta tttctaagca
      781 attttatgac tcatattatt caggtagaga aaaggcctac gcaataaaaa ttggtgaagt
      841 ggaaagatat aaagaaccaa ttcctatctc tgctctaggt agtaatatta agccaccaca
      901 atcatatctt tacctacctg cgtaagaatc ccggccaccg tgccgggttt tcttttgtcc
      961 cctcccctca tcacacaaac cgttcgaaaa accaccacaa cctcccttca gttatcgcta
     1021 tgcgatgcaa gtcacaaaat taattctttt tgctatcaaa cagttaatat caaaacacat
     1081 caatcaatat caataagtat tgatgcaacc aatagcaata gctattatca ccatgtcgca
     1141 acaacacaac gatacggcaa tcacctgatt caccgttgcg atgaccgctt agatccgcag
     1201 cttgaatttc agcaggctcc ggggagtgcg aggggtgaag cggacgcgtg aacgtcggtg
     1261 tgaccagctg aaatcaactc aacacctcat acctcagtcg cttcaacgag gcggcttagt
     1321 tatgacaacc ggcggccatc caccgcctga atacgcgcag aagtctctat atgttcagca
     1381 gcccagctta cgggcaggag tttttatggt tcatcaacat tacggaacgc agaccgttaa
     1441 tcgcggtgcg gtcatgccag gaatgctggt caaacacaaa gatggtacct ggactgcatc
     1501 agctaattta cgcggacggc tttatctgca tcgcggcatc gagcgcactt atacccgtga
     1561 tttgctcgtg gaagtttttc tcgacggacg cggcaacggc ctgaatcact aatccccttt
     1621 cctgtttacc taatcagccc ggcatttcgc aggcgatatt ttcacagcta tttcaggagt
     1681 tcagccatga acgcttatta cattcaggat cgtcttgagg ctcagagctg ggcgcgtcac
     1741 taccagcaga tcgcccgtga agagaaagag gcagaactgg cagacgacat ggaaaaaggc
     1801 ctgcccctgc acctgtttga atcgctatgc atcgatcatt tgcaacgcca cggggccagc
     1861 aaaaaagcca ttacccgtgc gtttgatgac gatgttgagt ttcaggagcg catggcagaa
     1921 cacatccggt acatggttga aaccattgct caccaccagg ttgatattga ttcagaggta
     1981 taaaacggat gagtacagca ctcgcaacgc tggctgggaa gctggctgaa cgtgtcggca
     2041 tggattctgt cgacccacag gaactgatca ccactcttcg ccagacagca tttaaaggcg
     2101 atgccagcga tgcgcagttc atcgcattgc tgatcgtcgc caaccaatac ggccttaatc
     2161 cgtggacgaa agaaatttac gccttccctg ataagcagaa cggcatcgtt ccggtggtgg
     2221 gcgttgatgg ctggtcccgc atcatcaacg aaaaccagca gtttgacggc atggactttg
     2281 aacaggacaa cgaatcctgc acatgccgga tttaccgcaa agaccgcaat catccgatct
     2341 gcgttaccga gtggatggat gaatgccgcc gcgaaccatt caaaacccgc gaaggcagag
     2401 aaatcacggg gccgtggcag tcgcatccca aacggatgtt acggcataaa gccatgattc
     2461 agtgtgcccg tctcgccttc ggatttgctg gtatctatga caaggatgaa gccgagcgca
     2521 ttgtcgaaaa taccgcatac actgcagaac gtcagccgga acgcgacatc actccggtta
     2581 acgatgaaac catgcaggag attaacactc tgctgatcgc cctggataaa acatgggatg
     2641 acgacttatt gccgctctgt tcccagatat ttcgccgcga cattcgcgca tcgtcagaac
     2701 tgacacaggc cgaagcagtg aaagctcttg gattcctgaa acagaaagcc actgagcaga
     2761 aggtggcagc atgacaccgg acattatcct gcagcgtacc gggatcgacg tgagagctgt
     2821 cgaacagggg gatgatgcgt ggcacaaatt acggctcggc gtcatcaccg cttcagaagt
     2881 tcacaacgtg atagcaaagc cccgctcagg aaagaagtgg cctgacatga aaatgtccta
     2941 cttccacacc ctgctggctg aggtttgcac cggtgtgact ccggaagtta atgctaaggc
     3001 gctggcctgg ggaaaacagt acgagaacga cgccagaact ctgtttgaat tcacttccgg
     3061 cgtgaatgtt actgaatccc cgatcatcta tcgcgacgaa agtatgcgca ccgcctgctc
     3121 tcccgatggt ttatgcagtg acggcaatgg ccttgagctg aaatgcccgt ttacctcccg
     3181 ggatttcatg aagttccggc tcggtggttt cgaggccata aagtcggctt acatggccca
     3241 ggtgcagtac agcatgtggg tgacacgaaa agatgcctgg tactttgcca actatgaccc
     3301 gcgtatgaag cgtgaaggac tgcattatgt cgtggttgag cgggatgaaa agtacatggc
     3361 gagttttgac gagatggtgc cggagttcat cgaaaaaatg gacgaggcac tggctgaaat
     3421 tggttttgta tttggggagc aatggcgatg acgcatcctc acgataatat ccgggtaggc
     3481 gcgatcactt tcgtctactc cattacaaag cgaggctggg tatttcccgg cctttctgtt
     3541 atcagaaatc cactgaaagc acagcggctg gctgagaaga taaataataa acaggaggat
     3601 atatgagtca ggttggtaat cattcattcg aatttccggc atcgcaaggt gtacagggtg
     3661 gtactgttac actcttcctt accataccag gaagatcgct ggctcgtttc ctcgcttcag
     3721 ataattacgg ccatacactg gcacgctctc agcgagaaat taatccaaat cgagtacgaa
     3781 aatttttaaa ttatctcact aacgcagact caagaaatga gccttttatc attccccctc
     3841 tcgtaggtaa ctgtgattcg aatatagaat ttgtaccgtt tggcaacaca aatgttggta
     3901 tagccagaat tcccctcgac gccgaaataa aactttttga tggtcaacat cgtgcagctg
     3961 gcattgagat attttgccga agttccccat caacgctcat ggttcccatg atgcttacaa
     4021 tgaatctgcc gctaaaaacc cggcagcagt tcttttcgga cataaataac aacgtttcta
     4081 agccatcagc gaccatcaat atggcgtata acggccggga tgatattgct cagggaatga
     4141 tatccttcct gacccaacat actgtatttg ccgatataac cgattttgaa cacaacgtag
     4201 tgccattaaa aagtaatatg tgggtgagtt tcaaggcact cactgatgca acgtcaaagt
     4261 tcgctaggaa cggcaatcaa caacttgaaa tgggatatat agaatctgtc tgggaggcat
     4321 ggattacact aactcagatt gactcaatcc gacatggtgt acaccacgct acgtacaagc
     4381 gcgattatat tcagttccat ggagtaatga ttaacgcttt cggttttgcg gttcaacaga
     4441 tgatggttaa tcattccatc gcagaaataa cttctatgat cgaaaaacta tgtgcaacta
     4501 ccagctctgc agaaagagag gatttttttc tgatggataa ctgggcgggg atctgcacga
     4561 aagccagcca ggaaaaacta tctgttattg ccaatgtggc agcgcagaaa gcagcagcaa
     4621 acagactgat acaagctttt accaaaggaa gtctggaaac aacttaatga atcaacattg
     4681 tctcatatca gcatgctgta cggcgtcttt aaggaacggt gagcatgaaa aacaaaatca
     4741 tcatggagct acaggctcct tttttattat tcgcattcac cctcaagcgt attaaccaac
     4801 aattcaggga ttaatgaaag atggcagaca tcattgattc agcatcagaa attgaagaat
     4861 tacagcgcaa cacagcaata aaaatgcgcc gcctgaacca ccaggctata tctgccactc
     4921 attgttgtga gtgtggcgat ccgatagatg aacgaagacg cctggccgtt cagggttgtc
     4981 ggacttgtgc aagttgccag gaggatctgg aacttatcag taaacagaga ggttcgaagt
     5041 gagcgaaatt aactagaagc caaagataaa atcatcgctg agcaggagaa aatcgctaac
     5101 ggagaaaaga cagtaagtca gtatatgaaa accgcatgat atcatcagat aaaaaccgat
     5161 cgtaaagcga aatattaata ccagaataaa cgagtcgagg taaattatat tacctcgata
     5221 aattaactaa aacttgcccg ctatatacta tctcattcag tatcatcacg cgcggtctgt
     5281 gcatatgtca ctaccgcacc taatgtatta attttctttt caacatagat aatattatcg
     5341 tactcataat tgccatacgg atagcaaatg cgaatattct catgtagatc ggggtcatcc
     5401 acctcagctc cagaacaact ttttgaacta ccggaagtat accgatacgg tgcaacataa
     5461 gacgatgtct ctccaggcaa aaaataagtt agtgttgtaa ggggtataat cagaaaaaat
     5521 ccagaaaata tgcacatccc tgcataaacc ttaaggtatg ctgacagact cttccagccg
     5581 ctttgtttta ctatcccctt cttaacccaa aacagagata acagaaaagc tattcccatg
     5641 ctaaacagaa tgtaatagtg ggatatactc tgattaagaa acgtgaccct gtagatatct
     5701 gcccgccacc agaagaaaag gaaaataaag atcagccctg aaactgtcat gcaaatcaaa
     5761 taaggatacg aatctttttt catgtttagc gcccataaaa tttttcctgc accggacaaa
     5821 tttaccatcc attttttgcg cagaaaatag ctcattactt actgcacaat aatacacaaa
     5881 attgcgtaaa ttttttgcat ggattttagc tctttcagcc gacgtttaag gggtaaatag
     5941 catttcctaa aagcaactgc accaacccaa cagaatgggc taccgcttac gttgagagca
     6001 aaaaagtgta tagcagcaat gaacagcatc ctcgcactga cggggatttc ttttatctga
     6061 gctcgctacg gcgagttttg ttttatggag acaagaaata tcagatttgg ctatgaaggt
     6121 tttgaaatgg caatcaactg gcgatgtcgg catcagtagc gcaactcttg cctcaatcgc
     6181 atgtggcctg aaaaagaata tctatggtca tcacttcggc gctccccatg acgcagcaga
     6241 ctttcggcga tgcgttgcac ttgttgagca gattccagaa atcagagatt cattcgacaa
     6301 ggttgcaaag cgcgttccgg cattcaaagg aatcctcaac gaatgggatt cactcgttgc
     6361 tctgttgaag tctgaaatga agacgtacgg gaacaaagca ccagagactt acagaagaat
     6421 cagcgagcta cgcaaggact aacccgcctc acactcgatg aggcattttc atttatcaag
     6481 atatccagac ctaccatcgc cgcatcaatg cggttttttt attacctgat ttgcaggttc
     6541 gattccctat tcggagatag cactcatgca acacgaacta cagcctgatt ccctggttga
     6601 tttgaaattc atcatggccg atactggctt cggtaaaacc ttcatctatg accggattaa
     6661 gtccggagac ctgcctaaag ccaaagttat ccacgggcga gcaagatggt tatatcgtga
     6721 ccattgtgaa ttcaaaaata agctcttaag ccgcgccaat gggtaaaata gcgggtaaaa
     6781 tatttctcac atctaaaaaa caccattcta atcaaccacc tgccgcctca agtagatgtc
     6841 tgcaggggac accagatacc cttcaaacga aatctacctt caccccgtaa aagatgggtt
     6901 tggcagcaca cttgccctat atctactcat ttttactgca acaggttgaa atctcagcac
     6961 tgtcagaaag cgctgatgac taaacagcct tgggccgggc gatgtaacca tcacacagaa
     7021 tcctgatagc gaaatatggc gtgactcgat acttcactcc gcaatgcatt ccttgatgaa
     7081 ttcgcaggcc cgtgatacac gggacaggtc gctgaatgac gacaatgtcc tggaaatcag
     7141 cgaaccgcgt atccgaagta catttgagcg actgtaccag aacataggtg attattagca
     7201 gggctaagca ttttagtatt attattttcc ggttgaggga tatggagata tcgacaacaa
     7261 ccggaaaaag tttacgtcta tattgctgaa ggtacaggcg tttccataac tatttgctcg
     7321 cgttttttac tcaggaagaa aatgccaaat agcaacatca ggcagacaat acccgaaatt
     7381 gcgaagaaaa ctgtctggta gcctgcgtgg tcaaagagta tcccagtcgg cgttgaaagc
     7441 agcacaatcc caagcgaact ggcaatttga aaaccaatca gaaagatcgt cgacgacagg
     7501 cgcttatcaa agtttgccac gctgtatttg aagacggata tgacacaaag tggaacctca
     7561 atggcatgta acagcttcac taatgaaata atccaggggt taacgaacag cgcgcaggaa
     7621 aggatacgca acgccataat cacaacaccg ataagtaatg cattttttgg ccctacccga
     7681 ttcacaaaga aaggaataat cgccatgcac agcgcttcga gtaccacctg gaatgagttg
     7741 agataaccat acaggcgcgt tcctacatcg tgtgattcga ataaacctgc ataaaagaca
     7801 ggaaaaagtt gttgatcaaa aatgttatag aaagaccacg tccccacaat aaatatgacg
     7861 aaaacccaga agtttcgatc cttgaaaact gcgataaaat cctctttttt tacccctccc
     7921 gcatccgccg ctacgcactg gtgatcctta tctttaaaac acatgttgat catcataaat
     7981 acagcgccaa atagcgagac caaccagaag ttgatatggg gactgatact aaaaaatatg
     8041 ccggcaaaga acgcgccaat agcatagcca aaagatcccc aggcgcgcgc tgttccatat
     8101 tcgaaatgaa aatttcgcgc cattttttcg gtgaagctgt caagcaaacc gcatcccgcc
     8161 agatacccca agccaaaaaa gagcgccccc agaattagac ctacagaaaa attgctttgc
     8221 agtaacggtt cataaacgta aatcataaac ggtccggtca agaccaggat gaaactcata
     8281 caccagatga gtggtttctt cagaccgagt ttatcctgaa cgatgccgta gaacatcata
     8341 aatagaatgc tggtaaactg gttgaccgaa taaagtgtac ctaattccgt ccctgtcaac
     8401 cctagatgtc ctttcagcca aatagcgtat aacgaccacc acagcgacca ggaaataaaa
     8461 aagagaaatg agtaactgga tgcaaaacga tagtacgcat ttctgaatgg gatattcagt
     8521 gccataatta cctgcctgtc gttaaaaaat tcacgtccta tttagagata agagcgactt
     8581 cgccgtttac ttctcactat tccagttctt gtcgacatgg cagcgctgtc attgcccctt
     8641 tcgccgttac tgcaagcgct ccgcaacgtt gagcgagatc gataattcgt cgcattcctc
     8701 tctcatctgt agataatccc gtagaggaca gacctgtgag taacccggca acgaacgcat
     8761 ctcccgcccc cgtgctatcg acacaattca cagacattcc agcaaaatgg tgaacttgtc
     8821 ctcgataaca gaccaccacc ccttctgcac ctttagtcac caacagcatg gcgatctcat
     8881 actcttttgc cagggcgcat atatcccgat cgttctgtgt ttttccactg attagtcgcc
     8941 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
     9001 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt gggatcgaag ctgacaaaac
     9061 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
     9121 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
     9181 tctctaaaaa aagatcggca ctggggcgaa ccataaacgt aaatgaacgt tccccttgat
     9241 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
     9301 tatcgactcc ctcagttagc agagttcttt gcattaacgc accaaaagga tcatcaccga
     9361 cccgacctat aaacccactt attccgccta atctggcgat tcccaccgca acgttagctg
     9421 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctactaccg
     9481 catcccctaa aacccatact ttggctgaca tttttttccc ttaaattcat ctgacttacg
     9541 catagtgata aacctctttt tcgcaaaatc gtcatagatt tactaaaaca tgcatattcg
     9601 atcacaaaat gtcatagtta acgttaacat ttgtgatatt catcgcattt atgaaagtaa
     9661 gggactttat ttttataaaa gttaacgtta acaattcacc aaatttgctt aaccaggatg
     9721 attaaaatga cgcaatctcg attgcatgca gcgcaaaacg cactagcaaa acttcatgag
     9781 caccggggta acactttcta tccccatttt cacctcgcgc ctcctgccgg gtggatgaac
     9841 gatccaaacg gcctgatctg gtttaacgat cgttatcacg cgttttatca acatcaccca
     9901 atgagcgaac actgggggcc aatgcactgg ggacatgcca ccagtgacga tatgatccac
     9961 tggcagcatg agcctattgc gctagcgcca ggagacgata atgacaaaga cgggtgtttt
    10021 tcaggtagtg ctgtcgatga caatggtgtc ctctcactta tctacaccgg acacgtctgg
    10081 ctcgatggtg caggtaatga cgatgcaatt cgcgaagtac aatgtctggc taccagtcgg
    10141 gatggtattc atttcgagaa acagggtgtg atcctcactc caccagaagg aatcatgcac
    10201 ttccgcgatc ctaaagtgtg gcgtgaagcc gacacatggt ggatggtagt cggggcgaaa
    10261 gacccaggca acacggggca gatcctgctt tatcgcggca gttcattacg tgaatggact
    10321 ttcgatcgcg tactggccca cgctgatgcg ggtgaaagct atatgtggga atgtccggac
    10381 tttttcagcc ttggcgatca gcattatctg atgttttccc cgcagggaat gaatgccgag
    10441 ggatacagtt accgaaatcg ctttcaaagt ggcgtaatac ccggaatgtg gtcgccagga
    10501 cgactttttg cacaatccgg gcattttact gaacttgata acgggcatga cttttatgca
    10561 ccacaaagct ttgtagcgaa ggatggtcgg cgtattgtta tcggctggat ggatatgtgg
    10621 gaatcgccaa tgccctcaaa acgtgaaggc tgggcaggct gcatgacgct ggcgcgcgag
    10681 ctatcagaga gcaattgcaa actcctacaa cgcccggtac acgaagctga gtcgttacgc
    10741 cagcagcatc aatctatctc tccccgcaca atcagcaata aatatgtttt gcaggaaaac
    10801 gcgcaagcag ttgagattca gttgcagtgg gcgctgaaga acagtgatgc cgaacattac
    10861 ggattacagc tcggcactgg aatgcggctg tatattgata accaatctga gcgacttgtt
    10921 ttgtggcggt attacccaca cgagaattta gacggctacc gtagtattcc cctcccgcag
    10981 cgtgacacgc tcgccctaag gatatttatc gatacatcat ccgtggaagt atttattaac
    11041 gacggggaag cggtgatgag tagtcgaatc tatccgcagc cagaagaacg ggaactgtcg
    11101 ctttatgcct cccacggagt ggctgtggtg caacatggag cactctggca actgggttaa
    11161 cataatatca ggtggaacaa cggatcaaca gcgggcaagg gatccgcgtc acacttcccc
    11221 cttcacgacc ttcaataata tgcaatgcag cttcccgccc gataatgtca tgtggaagct
    11281 gaattgtggt cagcggcggt aaaaacagat gcccgacgcc aaccagatta tcaaagccca
    11341 ttacggcgac atcctgcggg atacgtaccc ccttagccag gagaacctga taagccacaa
    11401 aggctgcgcg atcgttacca catatcagaa catcaaaatc tggtttgccc gatttgaagt
    11461 ggtcattgag taaacttgcg agatcggtgt agtgatcatc acctgttgcc atgtgaaatt
    11521 gtttcacctc agccagatct cgtccagcat cacgccaggc ctgctcaaat ccctgccgac
    11581 gataccctgt tgccaacgca ctttccggta gccagaagca taacggttga cgatagcccg
    11641 ccgcgagcaa atgctgtgtt gattcatatt gtgcagtgta atcatcaggg atataactgg
    11701 gtaacgctgg gtcatccgcc acacagttcg ccaatacaat attttcacca tacagagact
    11761 caggcagcgt gatatgtcgc agccccattg tagtatagat aatgccatcc ggacggtggg
    11821 caagcagctg acgtgccgcg cgggcagcgt catcttcaga aaaaatattg attaaaaaac
    11881 tattccagcc gaactcgctg gcggtttgct caatggcaag cagaatatca acagagaaag
    11941 gagtggtagc agtgtcctgc gccagcacgg cgagagtcga cggcttacgt ccttgagcgc
    12001 gcatcttacg ggcggaaaga tcaggaacat aattcagggt ctggattgcc tgcaatacgc
    12061 ggtcacgcgt tgcaggacgc acagattctg cattatgcat cacccgggag actgtcatca
    12121 tcgacactcc cgccaggcgt gcgacatcct ttaatgaagc catacccaag ccgtttgccg
    12181 taaaacgggc actgtagcag aaacagacgt cactggcgag atccaacgcc ctatcacctg
    12241 acacggcaat acaataaaaa ataacaataa ttcccggata attgtaccca attccgcctc
    12301 tgttctcgcc aacgagtaac catataggca agtaagtaaa acatgccaac tggcatcgcc
    12361 aacctggaga agaaaatata aatatggatc attgtgctgc cgctgtatcc cgacatcagc
    12421 ccggaaatta ttgcgattgc tatcggttca ggtgcgattg gctgcacgat cgttacggac
    12481 tcgcttttct ggctggtgaa gcaatattgc ggcgctacgc tcaatgaaac atttaaatac
    12541 tatacgacag cgacatttat cgcttcagtc gtcgctctgg cgggcacatt cctgctgtca
    12601 tttatcatct aagcgcaaag agacgtacta tggaaaacgc taaaatgaac tcgctcatcg
    12661 cccagtatcc gttggtaaag gttctggttg ctcttaaaga aaccacctgg tttaatcctg
    12721 gcacgacctc attggctgaa ggtttacctt atgttggcct gaccgaacag gatgttcagg
    12781 acgcccatgc gcgcttatcc cgttttgcac cctatctggc aaaagcattt cctgaaactg
    12841 ctgccactgg ggggattatt gaatcagaac tgattgccat tccggctatg caaaaacggc
    12901 tggaaaagga atatcaacaa ccgatcagcg ggcaactgct actgaaaaaa gatagccatt
    12961 tgcccatttc cggctccata aaagcacgcg gcgggattta tgaagtcctg gcacacgcag
    13021 aaaaactggc tctggaagcg gggttgctga cgcttgatga tgactacagc aaactgcttt
    13081 ctccggagtt taaacagttc tttagccaat acagcattgc tgtgggctca accggaaatc
    13141 tggggttatc aatcggcatt atgagcgccc gcattggctt taaggtgaca gtgcatatgt
    13201 ctgctgatgc ccgggcatgg aaaaaagcga aactgcgcag ccatggcgtt acggtcgtgg
    13261 aatatgagca agattatggt gttgccgtcg aggaaggacg taaagcagcg cagtctgacc
    13321 cgaactgttt ctttattgat gacgaaaatt cccgcacgtt gttccttggg tattccgtcg
    13381 ctggccagcg tcttaaagcg caatttgccc agcaaggccg tatcgtcgat gctgataacc
    13441 ctctgtttgt ctatctgccg tgtggtgttg gcggtggtcc tggtggcgtc gcattcgggc
    13501 ttaaactggc gtttggcgat catgttcact gcttttttgc cgaaccaacg cactcccctt
    13561 gtatgttgtt aggcgtccat acaggattac acgatcagat ttctgttcag gatattggta
    13621 tcgacaacct taccgcagcg gatggccttg cagttggtcg cgcatcaggc tttgtcgggc
    13681 gggcaatgga gcgtctgctg gatggcttct atacccttag cgatcaaacc atgtatgaca
    13741 tgcttggctg gctggcgcag gaagaaggta ttcgtcttga accttcggca ctggcgggta
    13801 tgaccggacc tcagcgcgtg tgtgcatcag taagttacca acagatgcac ggtttcagcg
    13861 cagaacaact gcgtaatacc actcatctgg tgtgggcgac gggaggtgga atggtgccgg
    13921 aagaagagat gaatcaatat ctggcaaaag gccgttaata acgtttcaac gcagcatcgc
    13981 catcctttcc ctgggtgagc gatgctgccg atggcgcaga cttaagatcc ccggtcttac
    14041 ccgctataac cccctgcttt taccatcacc caacgccttt cgctgtaaac ggcggtttcg
    14101 caaaccaaac caaaaccgta agtaagataa aagcaatagc cgccatacgg aaaatctcat
    14161 ttgcagaaat agaaagtgac tgctgggtta tttcattatt aatttcatta agaactcctg
    14221 aaagcgaacc atagtattta tccataattt gtgacgatga attaaacacg gggttaaatt
    14281 gatcgatggt tgctgtcaac tgactatggt gtaacgattc gcgtcgtccc cacagcgtca
    14341 ttgtcaacga cgtaccaacg gatcctgaca aggtacgaaa aaaattactc atactcgagg
    14401 cattggcaaa tttattatct ggcaagcctg aaaacgaaat cgttgttaag ggtaaaaaga
    14461 aacaggcaac ggcgaatccc tgaaaaaact gcggcaaaat gatacctgta aaatcaatcg
    14521 ttggcataaa tgtcacagaa cgccagtaat agcaaaccgc atacatcaaa aaactaaatg
    14581 tcaccaacac ccgcatgtca attttgttgc cataacgtcc tatcaaaggc gaaatcaata
    14641 gtggcatgat gccgatgggc gcataagcaa gtccggccca tatcgcatta taccccatcg
    14701 tttcctggag taactgcggc ataaggacta tcgctccaga gtaaaataaa tacgcgcagg
    14761 tgatactcac aataccaatg gtgaaattac gggacttaaa caaactgagg tcaagaatcg
    14821 ggttctctga ggtcgactcc caaatgacta aagagatcag agaaataact gatactactg
    14881 ttaatattat tattgtactc gagttgaacc aatccagatc gcgcccttta tcaagcataa
    14941 tttgcaagcc accaacaccg agcactaaca gggtcagtcc tggtagattc attttgaccg
    15001 gtgaagtctc agtttctctt cctttaagta aggttaagca taatgtcagg acgataatcc
    15061 ccatagggac attgattaaa aatatccaac cccagctaaa gttatcgcaa atatatccgc
    15121 ctaatatcgg cccacatatc ggagcgataa tcacggtcat tgaccataat gccagagcaa
    15181 atgttctttt ttctggtgga taattcctta ataataaact ctgtgacagt ggaattaacg
    15241 gccctgccat taacccctga acgactctaa aaaatatcag cacatcaaga ttggtcgata
    15301 aactacacat taatgaagac agcgaaaaaa aagtgactga aagtaaaaat aaccttaatt
    15361 cgcctattct ttgtgccagc ctgccggtaa cagggatcgc aatggcattt gctacgccaa
    15421 acgaggtgat aacccaggtg ccttcgtctg ttgatgctcc tagaaagcca gatattgtcg
    15481 gtattgcgac gttagaaata gtggaatcca acatttgcat aaacgtcgct aatgacaatg
    15541 caatagtgac gcaccataac gtcccaccgg ttaatggtgc cggagttgat ttagtgattg
    15601 ccattaaagt tgtccattat gcgaaataat gttgctaatt tctttttcta tcggactggt
    15661 atcgataact aaagccttac tggtataagc cggcatggac gtcacggttg aagccagctc
    15721 aggcatctca gcaatgtctt cgttcttcgt atcaatagtt gcagtcatcg ataaaccaat
    15781 gcgcaagggg tgttccatga gttcttttgg atcaagagaa acttcaaccg gtacacgctg
    15841 aacgattttg atccagttcc ctgtcgcatt ttgtgcaggt aataaggaga acgcattgcc
    15901 ggttcccata ttgatccctg tcacccggcc atgaaacaca acattctcac cataaagatc
    15961 gctgataata ttgaccgatt gaccaatccg tacatccgtg agttgtgttt ctttaaagtt
    16021 ggcattaacc cacatttgac gtgctggtac gacagccatt aacgattgtc cgggactcac
    16081 tgtttcgccg acctgaacac ttcgctgggc aatatagccg gtaaccggac tcttaatatc
    16141 cgtacgttta agcgccaacc aggcttcttt ggttgcatcc gcagcttcaa cgacttgtgg
    16201 ctgacggttt aatggtgtgt tcattactaa agctttattc gctttataag cctggatagc
    16261 ggcattcaat gccgctttgc tacttattaa cgtatctttg gtatgctcca gcgtttcttt
    16321 tgaaataacc ccctgcttcg ctaaaggcac acgacggtta taatcttcta aagattgttg
    16381 atactgaata cgtgctgaag cgacttcggc actgtattgt ttatcctgta agtacagttt
    16441 attcgtttgc cgaacaatat ttgccagatt atttttagct ttattgagtg cgatagtggc
    16501 atcagtttta tccagtgaaa ctaaaatgtc accctgtcga acgtagttcg tatctttatg
    16561 attaactaca gtgacactac ctgagacttg tgcagaaatt ggatctgcat tccccgtgac
    16621 ataggcgtca tctgtactaa tcatgtcttc taattccatt gaccaatagg cataagcacc
    16681 tgaaaacgca ataaataaaa ctatcgccaa taaagaaaag tattttcttc tgttagaatg
    16741 ttttttattt gaattaatct gttccacttt tatctctcat ttcgcataga tgaaatttat
    16801 gaattgaatg aaaagagtat tcattcttca tatcaaacac agtgcatgtg ttgcacatta
    16861 tcccaggaac gttggaggcc taatttaaat gaggtgcaaa aaaaacaaca gaggactaaa
    16921 ccgtggcttt tgcaatacaa ttcttacgcc tgtaggatta gtgagaagac ttatagtgct
    16981 aatgtgaaac tataaatcat cggtacaatc cctgatttta ttgttgacat ttcatatatg
    17041 ccgactattt atatggtata cttgtcgaat tatcttaaag gaagctcaaa ttttcttatt
    17101 tttattgaga aaatgagatg atgccttatg tctgtattac tacagggaga agggagatgc
    17161 ttcattgcaa agggaataat ctatgaacgc aataattatt gatgaccatc ctcttgctat
    17221 cgcagcaatt cgtaatttat tgatcaaaaa cgatattgaa atcttagcag agttgactga
    17281 aggcggaagt gccgttcagc gggtggaaac acttaagcct gatatcgtca tcattgatgt
    17341 cgatatcccc ggagttaacg gtatccaggt gttagaaacg ctgaggaagc gccaatatag
    17401 cggaattatt attatcgtct ccgctaaaaa tgaccatttt tacgggaaac attgtgctga
    17461 tgctggcgct aatggtttcg tgagtaaaaa agaaggcatg aacaatatca ttgcggctat
    17521 tgaagctgca aaaaatggct actgctattt ccccttctct ctcaaccggt ttgttggaag
    17581 tttaacgtcc gaccagcaaa aactcgactc cttatcgaaa caagaaatta gtgtcatgcg
    17641 ctatattctt gatggcaagg ataataatga cattgctgaa aaaatgttca tcagcaacaa
    17701 aactgtcagc acttataaaa gtcgcctgat ggaaaaatta gaatgtaaat cactgatgga
    17761 tctttacaca ttcgcacaac gtaacaaaat cggctaacca catgaagttt ttaccctata
    17821 tttttcttct ctgttgtggt ctttggtcga ccataagttt cgcagacgaa gattacatcg
    17881 aatatcgtgg catcagtagt aacaaccgtg tcacacttga tccactacgt ctgagcaaca
    17941 aggaattacg ttggttagcg agcaaaaaaa atcttgtgat tgcagtacac aagtcccaaa
    18001 cggctacgtt gttgcatacc gattcgcagc aacgggttcg tggtattaat gctgattatt
    18061 taaatctttt aaaaagagcg ttaaatatca aattaacact ccgggaatac gccgatcatc
    18121 aaaaagcaat ggacgcgctg gaagaaggtg aagtcgatat agtgttatca catttagttg
    18181 cttcgccgcc tcttaatgat gacattgctg caaccaaacc actgataatt acctttccgg
    18241 cgctggtcac cacccttcac gattcaatgc gaccgcttac ctcatcaaaa ccagtaaata
    18301 ttgctcgagt agcaaattat ccccctgacg aggtaattca tcaatcattt ccaaaagcaa
    18361 caattatctc ttttacaaat ttatatcagg cattagcatc cgtctcagcc gaacagaatg
    18421 attactttat tggtagtaac atcattacca gcagtatgat ttcccgctat ttcactcact
    18481 ccttaaatgt agtgaaatat tataactcac cgcgtcaata taattttttc ttgaccagaa
    18541 aagaatctgt cgttcttaat gaagtactca atcgatttgt tgatgctttg acaaatgaag
    18601 ttcgctatga agtatcacaa aattggcttg atacaggaaa cctggccttt ctgaacaaac
    18661 cattagaact cactgaacat gaaaaacaat ggattaagca gcatcccgat ttaaaggtgc
    18721 tggaaaatcc ttactcgccc ccctattcta tgacggatga aaatggctcg gttcggggcg
    18781 ttatggggga cattcttaat attattacct tgcaaacagg tttaaatttt tctccgatca
    18841 ccgtttcaca caatatccat gctggaacac agctgaatcc cggcggatgg gatatattac
    18901 ccggtgctat ttatagtgaa gatagagaaa ataatgtttt atttgctgaa gccttcataa
    18961 caacgcctta cgtttttgtc atgcaaaaag cgcctgacag tgaacaaaca ttaaaaaaag
    19021 gaatgaaagt tgccattcca tattattatg agcttcattc gcaattaaaa gagatgtatc
    19081 cggaggtaga gtggataaaa gtcgataacg ccagcgctgc atttcacaag gtcaaggaag
    19141 gcgaacttga tgctctggtc gcgacacagt taaattcacg ttacatgatc gaccattact
    19201 atcctaatga actttatcat tttcttattc ccggcgttca gaatgcatca ctttcgttcg
    19261 cttttcctcg cggagaaccg gaacttaagg atattattaa taaagcactg aatgcaatcc
    19321 ccccaagcga agttctgcgc ctgacggaaa aatggattaa aatgcccaat gtgaccattg
    19381 acacatggga cctctatagc gagcaatttt atattgttac gacattatcc gttttattag
    19441 ttggcagtag ccttttatgg ggattctacc tgttacgctc agttcgtcgt cgtaaagtca
    19501 tccagggtga tttagaaaac caaatatcat tccggaaagc actctcggat tccttaccga
    19561 atccaactta tgttgtaaac tggcaaggta atgtcattag tcataatagt gcttttgaac
    19621 attatttcac tgcggatttc tataaaaatg caatgttacc attagaaaac agtgactcac
    19681 cctttaaaga tgttttttct aatgcgcatg aagtcacagc agaaacgaaa gaaaatcgaa
    19741 caatatacac acaggtattt gaaattgata atggcatcga gaaaagatgc attaatcact
    19801 ggcatacatt atgcaatctg ccagccagcg atcatgctgt ttatatttgt ggttggcaag
    19861 atattaccga gacgcgtgat ttaattcatg cactcgaagt agagagaaat aaagcgatca
    19921 atgcaactgt cgcaaaaagt cagtttctgg caacaatgag tcacgaaata agaacaccaa
    19981 taagctccat tatgggcttc ctggaacttc tgtcgggttc tggtcttagc aaggagcaac
    20041 gggtggaggc gatttcacta gcctacgcca ccggacaatc actcctcggc ttaattggtg
    20101 aaatccttga tgtcgacaaa attgaatcgg gtaactatca acttcaacca caatgggtcg
    20161 atatccctac tttagtccag aacacttgtc actctttcgg tgcgattgct gcaagcaaat
    20221 cgatcgcatt aagttgcagc agtacatttc ctgatcatta cctggttaag atcgaccctc
    20281 aggcgtttaa gcaggtctta tcaaatttac tgagtaatgc tctcaaattt accaccgagg
    20341 gggcagtaaa aattacgacc tccctggttc acattgatga caaccacgct gtaatcaaaa
    20401 tgacgattat ggattctgga agtggattat cacaggaaga acaacaacaa ctgtttaaac
    20461 gttatagcca aacaagtgca ggtcgtcagc aaacaggttc tggtttaggc ttaatgatct
    20521 gcaaagaatt aattaaaaac atgcagggtg atttgtcatt agaaagtcat ccaggcatag
    20581 gaacaacatt tacgatcaca atcccggtag aaattaccca acaagtggcg gctgtcgagg
    20641 caaaagcaga acaacctatc acactacctg aaaagttgag catattaatc gcggatgatc
    20701 atccgaccaa caggctatta ctcaaacgcc agctaaatct attaggatat gatgttgacg
    20761 aagccactga tggtgtgcaa gcgctacaca aagtcagtat gcaacattat gatctgctta
    20821 ttactgacgt taatatgccg aatatggatg gttttgagtt gactcgcaaa ctccgtgagc
    20881 aaaattcgtc cttacccatc tgggggctta cagccaacgc acaggctaac gaacgtgaaa
    20941 aagggttaaa ttgcggcatg aacttatgtt tgttcaaacc gttgaccctg gatgtactga
    21001 aaacacattt aagtcagtta caccaggttg tgcatattgc acctcagtat cgccaccttg
    21061 atatcgaagc cctgaaaaat aatacggcga acgatctaca actgatgcag gagattctca
    21121 tgactttcca gcatgaaacg cataaagatc tacccgctgc gtttcatgca ctagaagctg
    21181 gcgataacag aactttccat caatgtattc atcgcatcca cggtgcggct aacatactga
    21241 atttgcaaaa gttgattaat attagccatc agttagaaat aacacctgtt tcagatgaca
    21301 gtaaacctga aattcttcag ttgctgaact ctgtaaaaga acacattgca gagctggacc
    21361 aggagattgc tgttttctgt caacaaaatg actaaatagc ggctcccaca atgttcaaat
    21421 gtgggagcta tttaccagca catcttacat ttatgatgag aactcctggc gaatttgttc
    21481 cccatgctgg tcgagcgttg ccgctcccgg cataacatgc gggtccgcgc agccactgat
    21541 ttttatcgga ttacccggca tcattattcc cccggcttca atcaacatat ttctcgcctg
    21601 agtttgtggc aaattaatgg cctcagccac acttaataac ggcgcgacgg gcacaccaac
    21661 ttcatgtatt ctggctaacc aaacttcagc tgcctgtgtt tttaacgtcc gctcaatata
    21721 ttgtttaaga atagcctggt tttgtacgcg taaaatattg ctgctaaatc tgggatcatt
    21781 aaccagttcc gtaagctcca gtgcctggca taacgcagag aaaagcttgt cattaccaca
    21841 acaaatcgta atcggcttat cctgagtatc gaaaacatca aaaggtgcca tgtaggggtg
    21901 gcgatttccc aaacgttgtg gtgacttccc agtcgcgata tatgccatca gcccgtgctc
    21961 cagaaaactc agcgtggcat caaacatcgc tatatcgaca tgcgcccctc tctggctctt
    22021 ttcgcggcca taaagtgcac tcactattcc actgaataaa tagacaccgc cacatagatc
    22081 cgcaagagaa gtgccaacgc gcactggcgg agcatcaggg tatcccgttt ccatcattat
    22141 cccgctcatt gcctgaatga tggtatcgta ggcaggagca tcttttagcg gaccggtatg
    22201 tccgaaacct gacgatgaag catatatgag gcgcgggttg atttcttgta acctttccca
    22261 tgaaaacccc agtttttcca ttgtacctgg gcgaaaattc tcagctaata catcagcttg
    22321 tttaagcata tttataaata tacttttatc gtgatcattc tttaaatcaa gaaccacact
    22381 ctctttacca tgattaataa aactgtaata gagtgactgt ccatccacat agggaccaaa
    22441 tgtgcgggta tcatcaccat gacctggtgg ctcaacttta attacccttg cgcccatatt
    22501 acaaagaagt tgagttccga aagggccatt aaggacatgt gtcatatcga taactaataa
    22561 gccttcaaac ggccctttgc tttcattatt tgtcatttgc ttatgccttg aaaaaagccg
    22621 aaggtgaaaa gacacttcgg catttatgta aaatcaataa catgattaat tatgaaacca
    22681 gtcgactgac ataaatccac aagggagccg tgacgacaaa acccagtacg ctcacagcca
    22741 atgacgctgt accagtgcgg gtgtagacat taaaccggct ggcaataatg attccggaga
    22801 atgccggcgg taatgcgcct gccagtacca tcatctgcag atgttcgctg ttcaaatgac
    22861 atgccatacc aacaagaagc agcgccagtg gcatcagaat cagcttcagg aaggtgttat
    22921 aagcaatttc ggcactgaac tcgaatttat gtgcagccag agtcaacccc gcagcgaata
    22981 ccgctacccc tgagttagcc ttcgcaatca gattaaaggt tgggtcccat gctgccggaa
    23041 tttttacccc aaccagcacc aggatcgttg ccagaacagg tgcccatact acaggctcct
    23101 ttgccgcaga aattaatgcg ctcagattac tattcttctt accatccgct cctgaggaag
    23161 gattcagcaa atacagacca ataggaatag taattgcgtt aacaataata gaaataattg
    23221 ctaccactaa acctgttgat acggaatcac cataaatagg atcgagaact gcaaatccca
    23281 ggaatccaat ggtaggtgaa cctgcaatta atgcacatac agctgcttct gcatgggtac
    23341 gtttaaaaaa tttgtagcaa ccaaaccagg agaaaaagaa acatccgaca ataaccacaa
    23401 gtgatacaag ggtcagacga gtgtccgcaa aaatcatttc cctgtttgcc cgggtaatag
    23461 aaacaaatag agccgcagga agagcgtagt ttaataccag tttattaaat gcccgcgctt
    23521 gatcttctga aaaagtttct cgtctgccgc taaaataacc caacagcatg attacgataa
    23581 taggcaataa atcgccaata aaaaatgtta gcataatagt atttccgata agggtcccct
    23641 caccttcgca taatgagttg gcaagaggag acgatgtcag tatttaatta ccagcgactt
    23701 gttttgggtt aagtttggta atatggccac tttcagttcc tgctgcaggg tcgatgacca
    23761 cattaataat ggtcggtttg cgcgactgaa taccggtggt taaagcatga cgaagttcat
    23821 ctgtcgtggt gacgttatag ccaacgccac gaaacgcatc cattaatttg tcataccttg
    23881 catggtgcaa cagatctgtt ggtgatggtg caccagcgcc actgagatca acaccgtctc
    23941 ctctgtagat gccgccatta ttaaaaataa cgatcgtcac cggcaggtta tatcgacaaa
    24001 tcgtttcaat ctccatccca ctgaaaccaa aagcactatc accttcaatg gcgacaaccg
    24061 gagaaccaga ggtcacgcta gcaccgatgg catagcccat accgatgccc atgacacccc
    24121 aggtaccaca atccagacga cgacgtggtt tatacatatc aataatattt cgtgcattat
    24181 ccagggtatt tgcaccttca ttaactaaat aaatatcctg gttctcacgc aatacatcgc
    24241 gcacagcact taatgcatta aagtaattta atggttgggt atctgtactt aatttttcat
    24301 gcattttttg tgcattttgc tgcttgtgga tatttaaaat atcgcgccat accagtggag
    24361 tcgtaaatgt gttttgtttc agttctgcca gcataccttg catactggat gcaatatcgc
    24421 caacgactgg cacagcaatg gggcggttgc tgtcaatttc ctgcggttca atatccagtt
    24481 gaataaactg tgtatctgcc gcccatcctt ttttaccgtg tgccagtaac caattcagtc
    24541 gtgcaccaac aagcatgaca acgtcagcat ttgccagggc aaacgaacgc gcagctgccg
    24601 cagaaagtgg atgcgtatct tcgaggatcc ctttcgccat agacattggc aggaatggaa
    24661 tctgggcact ttcaataaat tcacgaagct gttcgtcagc ttgtgaatac gccgcgcctt
    24721 tgccaaggat aattaatggc cgttcagctt ttgctaaaag cgaaattgcg ctagtgactg
    24781 acttcgggca tggcaataat gctggcgacg gattttcaac ttttacaatc gtggttaacg
    24841 cttcgtcttt ttccatcgtc gcggccagga catttgctgg caaatcaaga taaactccgc
    24901 cagggcgacc cgatactgag acccggatag cgcgtgccaa tgcaatgcca agatcctgcg
    24961 gctgattaac gcgaaatgct gctttggcat acggttttgc cgcattcatt tggtccagct
    25021 cttcataatc accttgctgt aggtcgacga tcgcgcggtc gctggagccg ctaatcatga
    25081 tcatcggaaa accatttacc gttgcgttgg ccaatgcggt caaaccattg aggaatcctg
    25141 gcgcagacac tgtcaggcag atccctggtt tttgggtaag aaaaccgctt gccgcagcgg
    25201 catagcctgc cgactgctca tgacgaaaac caatataacg aatgccttcc gcctgggcat
    25261 ggcgcgccat atccgtcaca ggaataccta caacaccata aatagtgtca atattattct
    25321 gttttaatgc ttcaacgatg atatgcatac catctgtcat ttgaagttga tctgacatga
    25381 ttaataacct cgaaattttt ataaattggg agccagaagc ccccgttaat atcagatggc
    25441 gtggttttgc ttcattgcag caatttcatc gtcgctataa cccagctcct gcaatacagc
    25501 agcggtatgt tcacctaata gcggcgcagc tttaatatcc ggcgtaaagt cagagaattt
    25561 cattggacaa ccaactgtca gatattttcc acgcaacggt tgttccactt cgacaacact
    25621 gccactttgg cgcaaagagg gatcaattga aatttctttc atacttaaaa ccggtgcaca
    25681 aggaatatca aattgagtca aataggccac cgcttcatgt ttatcaatag tgacagtgta
    25741 tttttcaatt tcagcaaaaa tatcgaaaat atgtggctgt cgggcatggg ctgtactgta
    25801 tgccggatcg gtaatccaat ctggtttgcc gatggcttta caggtgtttt cccagttttg
    25861 ctcctgaata gtgaaataaa tataggcgtt aggatcggtt tcccaacctt tacatttcag
    25921 gatccagcca ggctgaccgc caccacccgc attacctccg cggggaactg catcaccaaa
    25981 tgtgccattc ggatactgcg ggtattcttc cagataaccc aatttatcga gacgctgctg
    26041 gtcgcgtaat ttcacgcggc aaaggttcaa tacggcatcc tgcattgaca tggtgactcg
    26101 ttgcccacgc cccgtttttt cgcgatgcag caaagcagca agtaaaccga tcagtaaatg
    26161 cattccggtg ttgctgtcac ccaacgctgc agcgcttacc agcggcggac cgtcccaaaa
    26221 acccgtagtg gatgccgcgc cacccgctgc ctgagcaacg ttttcatagg cttttacatt
    26281 cacataaggc gaacactcat caaacccttt gatcgaacca aaaatcagac gtggattgat
    26341 ttcttgaata tgctcccagg tgaaacccat gtgatcaatg gcccctggat gaaagttctc
    26401 gactaagata tcagcttcgc ggatcagctt ttccattacc tctttgcctt ccgctgtttt
    26461 ggtatttaac tcaatagaac gtttgttact gttaagcatg gtgaagtaaa gcgcatcgat
    26521 atcaggaata tctcgcagct ggtgacgcgt tacgtcacca acgccgggac gttcaatttt
    26581 aataacgtca gcgccaaacc aggccagcat ttgagtacaa gatgggccag attgcacacc
    26641 ggtgaaatcg agaactttaa ttccttgaag tggagttgac atagcaatac ctattagtta
    26701 attaaatatg tgttaattta gcgtttattt aaggcataac gcctctataa ataatccgca
    26761 aaatatcgtc gtagcggcac tcgatatttt tacattcctg tatcaagtgc tgaaaatcat
    26821 ttcatcgcta cttccaattc ctttctgcat ggtaagtagt gaggacgaaa agaagaatat
    26881 gataaatatt ggttcgtggt aatcagagag agtcgcgaca tttgtgatgt tattcttaca
    26941 actgtaagaa gcgccggtga tttacaagta agggtatgat tacgcttttt aaagcgtacc
    27001 ataaaatata acatattaaa tgatttgtga tgacacggga ttattgcggt ttactttgag
    27061 gaatataaaa tgcgagttta tagaacagcg agcgccaaat aattttatga ccacaacctg
    27121 tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta cctcaatata
    27181 ctgcctgtca gcattagttc acaaacagtg cttcgctatc gacgatgatt ccatcttccg
    27241 cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt ttctgcgcat
    27301 cagcaagtgc attacgagtc tgtttaagcg gcatcagata ttggttttcc attacaccaa
    27361 caccggccag gcgaagctct tccattgcgc cctttttatc ccctttcgcc attttttcat
    27421 tggcaatttt tatcgcggct tctttttcgg gtgtcgctac ataactctca gaaattccga
    27481 cagatgcatt aatgacaata tattggtcat cattgacatt ggtcttttta cctggcttag
    27541 cgaactttgc ccattcggta ctgtcatctg acaacaaagc tgaagcttca ttggtcagtt
    27601 cttttgcctt ttccggatcg ccatgaaaca gagccaggcg cgctacctgc acgtcacgca
    27661 tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg gtttgctgtt
    27721 gtgccgctac cggtgcatta tcagcagccc atacagttga agatgccaga attgctgtga
    27781 ccatcgtagc cataattaaa cgtttcatac ttacctcatt ctttaacagt tttagaaaac
    27841 gccgccatca tattgggtga gcatgaaatg agaaatcaga gagcaacggt cattttgaaa
    27901 gggttatctt acagttgtag ttattatcgc aagaagtatt ttagtcttac aattgtagga
    27961 atatgcttcc tgtcacagag aactattctt atgtaccaca aagagatgtc atttattata
    28021 tcgatgtaaa tattgattaa ttatatgtaa atacagccat aacatcgtac attctcagtg
    28081 aaccattttc aggataactt tctatgaaag taaacttaat actttttagc ttatttttat
    28141 tggtctctat tatggcatgc aatgtttttg cattttctaa ttctggtggt ggtagtgaga
    28201 cgagctataa agagccagaa aaaacgtcag cgatgacgac aactcactct acaaaatttc
    28261 agccatcaca ggcgattttg tttaagatga gagaagatgc accaccatta aacctcacag
    28321 aagaaaccac gccccctttt ccgacaaagg cgagttatct tattcatcct gtgcgatagc
    28381 gtctgtcaca tggcaggtga gcaatcatgt ctgatgctga tgtgaacacc tgcccaaatg
    28441 agccatcctg acccactacc gcctgccata ccccaccata aagcgaatca tcaagccaca
    28501 agccggggca agaaacataa atatccccgt caccagttca ttaccgccag ttaaccacat
    28561 ggtgaagtaa taaatgagtg cagcgaggat caccatgccg aagaaagaag agaccgccga
    28621 taccacgttc agtacctgac cgatgtagcc gcttacgcag acaatacaca gggcgagaaa
    28681 catccataaa taaatcatag ctcaacctcc tgccgcgcta aaaaacctgt aacttcagtc
    28741 actttaatag taagacaatc cttaacctct ggcaattttt gctaagcatc aatgaaaaca
    28801 gcatgttaaa tgcaacacta ttgcatgcgg aaaatttttt actttgcacg attaataatc
    28861 agtagctgaa agcagtcagc cagtgataaa gtagagccac cgcattcatg ctcctcgtgg
    28921 ttatgtcctg actagtcttt acactcttta caggaaccat tgtcgtacat gatggcccaa
    28981 ccaattgagt gtaccgctgt cgacatggaa tcttctgcga tacaacaatt cgtatctaca
    29041 gaaggtaact atgtttccac aatgcaaatt ttcccgcgag tttctacatc ctcgctactg
    29101 gctcacatgg tttggacttg gtgtactctg gctttgggta cagcttcctt atcctgttct
    29161 ctgctttctc ggcacgcgta ttggcgcaat ggcgcgacca ttcctgaaac ggcgtgaatc
    29221 tatcgcccgt aaaaacctgg aactttgctt cccgcagcat tctgcggaag aacgcgagaa
    29281 gatgattgcc gaaaactttc gttcactcgg catggcgctg gtagaaaccg gcatggcgtg
    29341 gttctggccc gacagtcgcg tacgtaaatg gtttgatgtt gaagggctgg ataaccttaa
    29401 acgcgcacaa atgcaaaatc gcggcgtaat ggttgtcggc gtccatttta tgtcgctgga
    29461 actgggcggc cgcgtgatgg gactttgtca gccaatgatg gctacctatc gtccacataa
    29521 taatcagctg atggaatggg tgcagacccg tggtcgcatg cgctctaaca aagcgatgat
    29581 cggcagaaat aatctgcgcg gcattgtcag tgcactgaag aaaggtgaag cggtatggtt
    29641 tgctcccgat caggattatg gtcgtaaagg cagctccttc gcgccgttct ttgcggtaga
    29701 aaatgtcgcc acaaccaatg gcacctatgt tctctcccgt ctctctggcg cagccatgtt
    29761 gaccgtaacg atggtaagaa aagcggatta cagcggatat cgtttgttca tcaccccaga
    29821 gatggaaggc tacccgacag atgaaaatca agccgctgcc tatatgaaca agattatcga
    29881 gaaagaaatc atgcgcgcac cggagcagta cctctggatc caccgtcgct ttaaaacgcg
    29941 cccggtggga gaatcgtcgt tatacattta acaaactgct ctgactcttt acctcctcac
    30001 ataagccata ataacctcat ggtttttatg gcttttttct taaggttttt tccttcgctg
    30061 aatatctaat tattaccgtc agaaattaaa ctgtcgccga tatgagacac ctgtaagtta
    30121 ctaaaagtaa ataagaaaat gcctcttgtc tcgtttcagt tttaggcgta cattagcgcc
    30181 gtctcgagca gtttagctca gaattctggg gtgatgccca cggtaaaata caggactttt
    30241 aaggtcgttt tgcccggtgt caactcactg tatcaggtgt aatgaagtca ttcaggcgta
    30301 acagcaatta cgcggagaga tgtaaagtga aatatttctt tatgggcatt tcttttatgg
    30361 tcatcgtttg ggccggtact tttgccctga tgatctaaaa gcaaactgtg caatgaaaaa
    30421 caggagccat caggctcctg tttgcatttc tgaggtcggt gtttattccg cgttttcgtg
    30481 aatatgtttg ctgctggcgg gtagtaaacc gtcggcacgg aacatcgctt taatcccacg
    30541 aatcgcctga cgaatacggt cgcggttttc aatcagtgca aagcgcacat gcgtatcacc
    30601 atagtcgcca aagccaatcc ccggagagac acagaccttc gcttcgttaa gcaatttctt
    30661 ggcaaattcc agcgagccca tggcagcata cggttccggt attttcgccc agacatacat
    30721 cgaagccttc ggcatttcga ccatccagcc cgcttcatgc agccctttaa ccaatacatc
    30781 acggcggcgt ttgtactgtt cggcaatgtc gcgcacgcac tgttgatcgc cctccagcgc
    30841 cgcaatcgcc gccacctgca acggcgtaaa ggtgccgtaa tcgtgatagc ttttaatacg
    30901 tgccagcgca ctgaccagcg ttttgttgcc aaccataaag ccgatacgcc agcccgccat
    30961 gttgtagctt ttcgacagcg taaagaactc gaccgccaca tcgcgtgcac ctgacacctg
    31021 catgattgac ggcgctttcc agccatcgta gacgatatcg gcataggcca ggtcatggac
    31081 caccagcaca tcgtagcgct tcgccagcgc cacaaccttt tcgaagaact ccagttccac
    31141 gcattgcgcg gttgggttcg acgggaagcc gaggatcatc atcttcggtt tcggataact
    31201 ttcacgaatg gcgcgctcca gttcgttgaa gaaatcgaca ccttccacca gcggcactga
    31261 gcgtacctga gccccggcaa tcaccgcgcc gtaaatatga atggggtaac ttggattcgg
    31321 caccagcact gtgtcaccgt gatccagtgt cgccagcatc agatgcgcca ggccctcttt
    31381 cgaaccaata gtgacgatgg cttctgattc cgggtcgatt tcaacgtcgt agcgatcctg
    31441 ataccagcgg gaaatggcgc gacgtagccg cggaataccg cgtgaagtgg agtaaccatg
    31501 cgtgtccggg cgctgggcca cagtacataa tttttcgacg atatgcggcg gagtcgcacc
    31561 gtccgggtta cccatactga aatcgataat atcttcgccg cgccgacgcg cagccatttt
    31621 cagttcagcg gtaatgttaa aaacataggg cgggagacga tcaatgcgcg taaagcgacg
    31681 ttcagggcga gtgtcagcca taatttcctc agattaacgt tagcgcccgg accgtccgag
    31741 cgacgctgcc acgatggtgg ctgttttgaa aatagcctga ttaatttctg tctgtccagc
    31801 gttttagaac atttttatta atgacgttaa atgaggaact ctgctttaaa aacaggaaaa
    31861 ttatctgcgc taacaagaaa attaccactc attttattaa cataataatt tcaataaatt
    31921 tactaaccag aagtgcatta tcaagagatg cattgctaat acaccattta tctatcaatc
    31981 cccttttaaa attgtggttt ttcctcaaaa acggcctgct ggtcatcagc cttcaggtta
    32041 cctatcatag aggtttaatc cttattcaga gtcacccgtg cacgaaatat tcaacatgct
    32101 gctggcggtc ttcgatcggg cagcgttaat gcttatctgc ctgttctttc tcatccgtat
    32161 ccgcctgttt cgcgaactgt tgcacaagtc ggcacactca ccgaaagagt tgctcgccgt
    32221 taccgccatt ttctcgctgt tcgccctctt cagcacctgg tccggcgttc ccgtagaagg
    32281 ctcgctggtg aacgtacgta ttatcgcggt gatgtccggc gggattctgt ttggcccgtg
    32341 ggtgggcatc attaccggcg tgattgcggg tattcatcgt tatttaattg atatcggtgg
    32401 cgtgacggcg atcccctgct ttatcaccag cattctggct ggttgtatat cgggctggat
    32461 caacctgaaa atccccaaag cacagcgctg gcgcgtcggt attctcggcg gcatgttgtg
    32521 tgaaacgttg accatgattc tggtgattgt ctgggcacca actaccgcgt tggggatcga
    32581 tatcgtctct aaaatcggga ttccaatgat cctcggcagc gtctgtatcg gctttattgt
    32641 gcttctggtg caaagcgttg agggcgaaaa agaggccagc gccgcgcggc aggccaagtt
    32701 ggcgctggat atcgccaaca aaacgctacc tctgtttcgc catgtcaata gcgagtcatt
    32761 acgcaaagtt tgtgaaatta ttcgcgatga cattcacgcc gatgcggtgg cgattaccaa
    32821 taccgatcat gtgctggcct atgttggtgt gggtgaacat aactatcaga atggcgatga
    32881 cttcattagc ccgactaccc gtcaggcgat gaattacgga aaaatcatca ttaaaaacaa
    32941 tgatgaagcc caccgcacac cagagattca ttccatgctg gtgatcccat tgtgggagaa
    33001 aggcgttgtg accggaacgc tgaaaattta ctactgccac gcgcatcaga taacctcgtc
    33061 attacaggaa atggcggttg gtctgtcgca aatcatctcc acgcaactgg aggtttcacg
    33121 cgccgagcag ctacgtgaaa tggcaaataa ggcagagctt cgcgccctgc aaagcaaaat
    33181 taatccccat tttctgttta acgctctgaa cgctatttca tcgtcgatcc gtctgaatcc
    33241 ggataccgct cgccagctga tctttaatct gtcgcgttat ctgcgctata acattgaatt
    33301 aaaagacgat gagcaaatcg atatcaaaaa agagctgtat caaattaaag actatattgc
    33361 cattgagcag gcccgctttg gcgacaagct cacggttatc tatgatattg atgaagaggt
    33421 gaattgctgc attcccagcc tgctgatcca gccgttggtg gagaatgcca ttgtccacgg
    33481 tatccagcct tgcaaaggta aaggcgttgt caccatcagc gttgcagagt gcggcaatcg
    33541 ggtacgcatt gccgtgcggg ataccgggca cggcatcgat ccaaaggtga ttgagcgggt
    33601 cgaagcgaat gaaatgccgg gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa
    33661 gttattgtat ggcgaggggc tgcatatccg ccgcctggaa ccaggaacgg aaattgcgtt
    33721 ttacattcct aaccaacgca ccccagtcgc ctcacaggct acgttattgc tttgagccgg
    33781 agtgatattg tgaaagtcat cattgttgaa gacgaattcc tggcacaaca ggaactgagc
    33841 tggctaatta aagagcacag ccagatggag attgtcggca cctttgacga cggtctggac
    33901 gtgttgaagt ttttgcagca taaccgcgtc gacgccattt ttctggatat caatattccg
    33961 tcgctggatg gtgtgttgct ggcgcaaaac atcagccagt tcgcccataa accgtttatt
    34021 gtgttcatca ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac
    34081 tacattctca aaccgtatca ggagtcacgc attaccggga tgctgcaaaa actggaagcg
    34141 gcctggcaac aacagcagac cagcagcacg cctgccgcga cggtaacgcg tgagaatgac
    34201 accattaatc tggtgaaaga tgagcgaata atcgtcacgc caattaacga tatctattac
    34261 gccgaagcgc acgagaaaat gacctttgtc tatacgcggc gtgaatccta cgtaatgccg
    34321 atgaacatta ccgaattttg cagcaaactg ccgccgtcgc attttttccg ctgccatcgc
    34381 tcattttgtg tcaatctgaa caaaatccgc gagatcgaac cgtggtttaa taacacctat
    34441 attctgcgac tgaaagatct ggattttgaa gtgccagtca gccgcagcaa agtaaaagaa
    34501 tttcgccagt taatgcatct ttaatcggga actttcatga aagcaccagg cttgcccgcc
    34561 gatcaacaat ttttcgccga tctgttcagc ggcctggtgc ttaacccgca actactgggg
    34621 cgcgtctggt ttgccagcca gcctgcctcg ttgccggtgg gcagtttatg tattgatttt
    34681 ccccgtctgg atatcgtgct gcgcggcgaa tacggcaatc tgctggaagc aaagcagcaa
    34741 cgtttggtgg aaggagaaat gctgtttatt ccggcgcgcg cggctaattt accggtcaac
    34801 aacaaaccgg tgatgctgtt aagcctggtg ttcgctccga cctggcttgg gttatcgttt
    34861 tacgatagcc gcaccacgtc gttgttgcat cctgctcgcc agatccagct tcccagcctg
    34921 caacgcggtg aaggtgaagc gatgcttacc gccctcaccc atcttagccg ttcgccgctg
    34981 gagcaaaata tcattcagcc gctggtgtta agtttgctgc atctttgccg caacgtggtg
    35041 aatatgccgc cgggtaattc gcaaccgcgc ggcgattttc tctatcacag catttgtaac
    35101 tgggttcagg ataattatgc ccagccgctc acccgtgaga acgtggcgca gttttttaat
    35161 atcacgccca atcatctgtc aaaactgttt gcgcagcatg gaacaatgcg ttttatcgaa
    35221 tatgtgcgtt gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata tcatctgtcg
    35281 attcatgaag tggcgcagcg ttgcggcttt ccggacagcg actatttttg tcgcgtcttc
    35341 cggcgtcagt ttggcctgac gccgggagag tacagcgccc gttttcaggg ctaacgtcag
    35401 aaggttaatt ctgtttccag cagcgtcagg atactttgtg catcttgcgc ggcaaacaac
    35461 gactggcgga agtttttatt caccagttta cgcgccagct gcgagaagac tttcacatga
    35521 ttcatccctt cgttagcgcc cagcgtcagc atgattacca gttcgacttc gcccatctct
    35581 gactgccagt caaccggctt caccagccgg gcaatgctga tactggaatg acggatccac
    35641 tgagatttgg tgtgcgggat cgccacgcca aaaccaacgc cggtggtgac aatctcttcc
    35701 cgctgccaga catcctcttc cagctcgaac ggatgttcag tgcgcccgat aacgccgagg
    35761 ttaccgcaca ggaactggat cgcctgctct ttattgctaa aggcctgatc aacgaagata
    35821 ttctccagcg ccaacaatgg gcgaacgtct tcttccggcg taaaggcggt gagtaacgct
    35881 tcgatttcct gcgcgctgcg acattcacac gcctgacgtg ccagttcccg acacgcctcg
    35941 ctatccagtt gacgcagctg gcttttcacc gccggaatgc gcgggctact catactcagc
    36001 tcatccaggt ctagcccaag cagtagcggc agataacgga tttcaccgcc cagttcaccg
    36061 caaatgccta cccatttgcc ccgctgatgc gcagtggtaa caatctgctg caacatgcgc
    36121 aggaacgatg gcgtaatcgg gttataaagc ggcgatacgc gcgggttatt acgatcgacc
    36181 gcatacagat actgggtcat atcgttggag ccgatactga agaaatcgac ctcatcgcag
    36241 aagtgatcga tgatgtagca caccgacggc acttccacca tgatacccag cgtaatcgtc
    36301 tctgcatgac gcaggccatc gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc
    36361 acccataaga tctgatcgag gctgtgaacc atcgggatca tcaactgggc gttgccgaaa
    36421 ctggcggcgc gcaaaatggc ccgcagttga gtgcggaaca ggccagcaaa ttccgggtaa
    36481 atacgtaccg cgcgatagcc gaggaacggg ttctcttcct ggggaatatt cagataagga
    36541 atgcttttat cgccgccgat gtccattgtg cggaagataa tcggcttgtc gcccgccgcc
    36601 agtagcacct gctggtaggc ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc
    36661 atatagagca tttcggtacg gaacagcccg acgccttccg cgccgttggc aaacgcgcca
    36721 ggcgcttcca gagcggtgcc aatattcgcc gcgatatcaa tacgcttgtt atcacgggaa
    36781 taagccagct gcgcggcagc ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgc
    36841 gcgacctgat aataaccgct caccgcgtca ttcgggttaa tcgccagcac gccgcactgg
    36901 gcgtcaagca ctgcaggttg cccggcataa cgggcaatcg catccagcgg caggccgctc
    36961 agtaccggga tcgccgaggc gcgggccaga atcagggtat gcgaggtgcg cccggttttc
    37021 tccagaatca tgcccgcaag attttttaaa tccaggctta aaaactggct tggggttaaa
    37081 tcctcggcca ccagaatagt cggtttttcc agcaccagat tgttgcgcgg cttcagttcc
    37141 ggccaggtaa tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc
    37201 agataatcgc tggcagaagc agaaagtttg gcgcaaacct gctccatatt gctgatgatc
    37261 gccgccccca gcccctgatg ctgttctgtc atcaggcgac ggatattgcc tgcaaattca
    37321 tcatcctgaa tcagcgacaa atgggcgctg aggatagttt tgctttcgcc gtcacgctca
    37381 cgcaattgct ggttcagttg ctcggcaagc gttgccaggc tgtgctccag ccgggtggaa
    37441 tcttgcgcac tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc
    37501 agcgtaccca cgccgacgcc gcttgccagc acattgccgt acagtaaatc cgggttaagg
    37561 cggcttaatg aacgcggcag cggatgcgcc gtcagttctg cctgcgtagg ctgaacgctg
    37621 tcgctgtcga taaagcgcac ctggatgtac tcttccagca cgcgccgcgc ctgctcttca
    37681 tcgctgccgc taatgttcag gctgcaactg tcattaaata gggtgccggt gccaatcagc
    37741 gccagcgagc ttttggcatc ggcctttgcg ttctggcgat ggttaataaa agtgatttcg
    37801 ctttgccact ggctgcactg ttctttaagt tcccacgccg gacgagcatg tagaccgtta
    37861 ggcaaaggac agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat
    37921 ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac gttgaataag ggcagataaa
    37981 agttgctcca tctgcaaaat gtcgcggcaa tcggcaatcg atgcggcgca atgtccgtgg
    38041 cgggtggcag gccccatcac cactgtgggt acgccggtac cggttaagtg caccgctcca
    38101 ccgtcagtgc cgccgttact gaacatatcc gcctgtaacg gcacgccaat ttctgccgcc
    38161 acggtttcga tccaggcggt gagttttggc ggcgcaatca gtgacttgtc gcttaacacc
    38221 agcatcgggc cgttaccaat ctggcgatgg ttagccgcgc cataatcaaa gtttttcgcc
    38281 cagcaggcgg tatcaaggac aatggcgacg tccggcgaca ccgcgcgggt ggcagtttgc
    38341 ccgccgcgta atcccacctc ttcgctggaa cttgccacca gccacacttc cgcaggcagt
    38401 tcggcgtcgt gcagctcacg tagtaacgtc accagcagat agcaaccgag gcggtcatca
    38461 aaggctttcc ccatcacccg ctggtggggg agaacctgaa aagtggtatc gaacgtgacg
    38521 cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat acgagcgcgc gccaatatcc
    38581 acgcgcatgg cgctgacatc attcccctgc cggtcgccat caagcagacc gggaattttg
    38641 cactcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgtacgttg
    38701 ccaaccggca gtacatcaat cgccccttcg cgggagatgc tgcgcaccat aaatcccact
    38761 tcatccatat gcgcacagat catcaccttc ggacctgtcg attcattcag gcggatcagc
    38821 accgatccca gaccatcaaa tcgcacttct ttttgcaggc gatccgcttc ttccagcagg
    38881 atctgccgta cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat
    38941 agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca
    39001 cttctgcgcc ttgcggggtg accagcacaa catcctcgat gcgcacgccc ccttgccctg
    39061 gcaaataaat ccccggctcc acggtcagta acatgcctgg ctgtagcgtc gtggtatccc
    39121 gcggtgaaaa gcgcggatct tcgtgtactt caatgccaat agcatgaccg gtgttgtgac
    39181 caaaatagtc gccaaatcct gcctcggtaa tcacccgacg cgcggcttcg tcaacctgct
    39241 ggcagcgcac accggggcga attgcagaga ttgctgcgag ctgtgcctgc aaaacaatct
    39301 gatagacgtt aaacagcgga tgagattcgg tgctcacccc ttcgccattc accagcaagg
    39361 tgcgcgtcat atcagagcag tagccctgat acagcgcacc gaaatcgaga gtgacaaact
    39421 cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg cagcgcccca cgccagccac
    39481 tggcaacaat ggtgtcaaaa gaggtttttt ctgcgccctg ctggcgcata aaccactcca
    39541 gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctctg
    39601 caccgcgatc ggcaatccca caggcaaggc ggattttctc cacctcctct ggcgttttga
    39661 tttgccgcag cacatccggc gtggcgctaa ccagtttcgc attgagttca gactgccagc
    39721 ggtgcgcggt ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgttcat
    39781 cggcaatgat ttcattgacg atggttgcaa gcgtgtgtgt cgcgtcaagc aaatgcagct
    39841 ggtaaccttg cgtacgagct tctacatccg cgtaatagcg cgagtccacc agaatgtgcg
    39901 cactttcacg gctaatcagc acatagcctg atccggtgga gatccccaga tgcggctgtt
    39961 tgttctgccg tgaggagaga agcactgcat ccagttgttg cgccttaagc cagtcgcgca
    40021 gcgaagcgag taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc
    40081 tttacggaac atcatcagac gcaggaacac caccatcagc gcagtaatga ctgcccccag
    40141 cgcgatcccc gccatataga cgcccaggtt agtgaccagc ggccatgccc agatagcgga
    40201 ttccgggaac cattgcactg cgcccagcca gacagcggcg gttgagccga caatcgcgcc
    40261 gaccatatac gacggaatgg cggtgatggg gctttccagc gcaaacggaa tcgcaccctc
    40321 actgatcccc ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa gttgtgcgtt
    40381 gaacaggcgt ttgccggtta aacgacggtc aataatggtc gccagcccca gaccaatcgg
    40441 cggaataacg atagcgatag aacgtgcggt gaccggtaat acgtggtcag tggtaaagct
    40501 gaaggcaacg aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc
    40561 ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacagtacg
    40621 gataccgccg ttgatccagc caccaaaggg ggtgatgacg tagtacatcg ccagcatgac
    40681 aaaaatagcg gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg
    40741 taactggatt ttctggttca tccatttcac aaggtaacca gcaacgatag aaataatcag
    40801 ggcaccaatg aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc
    40861 gaagttcagc agttgcgtcg gctgggtaga catcaagccg ccgataaaac cagccgggaa
    40921 cgccagtttg ccaccgatag agttcgccac aaatgcagca aacatcggaa tggcaaaacc
    40981 aaacaacacg ccgccgaagg actgcgacaa ccaggcaaat ttcagtaagg agaggtcgaa
    41041 accggagaat ttcccgctat taagagcatc catgatgccg atatcagcag gaattttaag
    41101 ccagctataa gcaatcagct ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg
    41161 caccatgcgt gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt
    41221 agaggcctgc ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc
    41281 actgcgtttt ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc
    41341 aatcatctct tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt
    41401 gatttcataa acgtcgcgtg attcgaaacg ttcgttatct tccggggtaa ctgccacgga
    41461 atggatgatg atggtcgctt cggcgatatc ctgcgccgtc aggcgattct ggataccgtc
    41521 cgcgccctgg gtttcaattt tcacttcata accggcttct accgctgctt cttccagcgc
    41581 ctgagcggcc ataaaggtgt gagccaggcc catcgggcag gcacataagg caatcagttt
    41641 cttactcatc gttaattcct cgttcagtga tagttgacga gtgaaattat gaacgtgcaa
    41701 aacaaatcgc cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg
    41761 aagtgtgacg caggtcggcc ttgtgagggg acacaaagaa aaattgaatt aactgtatga
    41821 tttaaaagat tatcgggaga gttatctccc gatataacag gaaggattta cagaatgtgc
    41881 cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg
    41941 agatacaccg gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcggca
    42001 cggaaaccag aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac
    42061 acgccgccaa atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg
    42121 caaaacagcg acaacgcacg gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta
    42181 atatcttttg gcttgagatt ttctggcagg cggttgtcgg ctttaacaat tgcgcgatac
    42241 aaattcacta gcccagggcc agaaagcacg cgttccgccg aaacatggcc aatttccgca
    42301 cgcagtattt cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg
    42361 ccttcgcccg gcaggcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc
    42421 cccgttccgg caccgtaaac cgcaataggc ttaccttcta tcggctctgc gccaccaaac
    42481 tgaatcagat gctctttttt cagcatcgga atcgccattg atacagcggt aaaatcgtta
    42541 ataatttcca gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag
    42601 gtatggttgg tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg
    42661 tctttcacct cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg
    42721 taatcaagcc ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga
    42781 gcaagacgtg cgttggtgcc gcccacatca ccgactaatg catactttgt cattcttcaa
    42841 ctgctccgct aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa
    42901 caacgccgga aaggcaggct ccctgtaaat atcgatctgg gtcacacaat tactttatcg
    42961 tttcagcacc aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc
    43021 gcataaggaa tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc
    43081 cgccgtggca attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc
    43141 ggtattacag aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc
    43201 acccctctgg atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg
    43261 tttcagccag ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccggt
    43321 tccgccctct gcgctacctg gacttatcgt agcattaatt ctcggtcttg ctggcggcgt
    43381 cagcctgggg ccggaacatc cgatcatgac cgtcaatatc gcccttgcgg ttgccattgg
    43441 cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac
    43501 catcggcgcg ctgtttggta cacctgttgc ggcggcgttg atattttcgc aaaccttaaa
    43561 tggcagtagt gaagttccgc tatgggatcg tctttttgcg ccgttaatgg cggcagcagc
    43621 tggtgcactt actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta
    43681 cggacagatg gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc
    43741 agcagggatg gtcgccgtat ggtgcttacc acggttgcac gcgatgatgc atcaaatgaa
    43801 aaatccggtg ctcgtgctgg gtattggcgg atttattctc ggtattctgg gggttattgg
    43861 tgggccggtt tcgctgttta aaggactgga tgagatgcag cagatggtgg caaatcaggc
    43921 tttcagcacc agcgattact ttttgctggc ggtgattaaa ctgaccgccc tggtcgttgc
    43981 tgccgccagt ggctttcgcg gtggacgaat tttcccggca gtgttcgtcg gcgtggcatt
    44041 agggttgatg ctgcatgagc acgtccccgc cgttccagcg gcaataaccg tttcctgcgc
    44101 tattctcggc atcgtgctgg tggtaacacg cgatggctgg ttaagccttt ttatggcggc
    44161 agtcgttgta cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg
    44221 gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agcccccccc
    44281 acgataacgt ttagcaaaaa atgctctcct ttatgattta agagttatgg cgatgatacg
    44341 gggctttacc tccccgtaat attgcgttaa caggccgctg acggcttcga tcgtgaagga
    44401 gaataacaat gttcaggtca ctgtttctgg cggccgccct gatggcattt accccgcttg
    44461 cagcaaacgc aggcgaaatc accctactgc catcaatcaa attacaaatt ggcgatcgcg
    44521 atcattacgg taattactgg gacggtggtc actggcgcga ccgtgactac tggcatcgca
    44581 attatgaatg gcgtaaaaac cgctggtggc gtcatgataa tggctaccat cgtggctggg
    44641 ataagcgtaa agcatatgag cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc
    44701 gcggaaaagg ccgcggacat ggacaccgcc attaaacaca ttcgccaatg gagcacaatg
    44761 cctgatgcga cgctagcgcg tcttatcagg catacaaacc ggctctttaa cgcgtagggc
    44821 ggataaggcg tttacgccgc atccgccaat agtgccagat gcgacgctca ttcaactaca
    44881 atcccagcgc cgtccccacc aacaaccaga tattcagcgc cacgaccagc actacaatca
    44941 cccagcctgt ctgttttaca cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac
    45001 tggtgaaaat cagcagtgga accagcgcca gggcgatacc aaaacttaac agtacctgac
    45061 tcataaccag aatccgtgtc ggatctaatc ccatcagaat gacaataaat gacggcagca
    45121 tggtgactgt acgacgcacc cacagcggga tatgaaagcg aatg
//
LOCUS       DAZGBC010000016        44736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGBC010000016 DAZGBC010000000
VERSION     DAZGBC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.016"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(276..818)
                     /gene="ycfP"
                     /locus_tag="ACHK1J_000959"
     CDS             complement(276..818)
                     /gene="ycfP"
                     /locus_tag="ACHK1J_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO7910272.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(841..1866)
                     /gene="nagZ"
                     /locus_tag="ACHK1J_000960"
     CDS             complement(841..1866)
                     /gene="nagZ"
                     /locus_tag="ACHK1J_000960"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO7910273.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1877..2701)
                     /gene="thiK"
                     /locus_tag="ACHK1J_000961"
     CDS             complement(1877..2701)
                     /gene="thiK"
                     /locus_tag="ACHK1J_000961"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO7910274.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLHDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENAEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2682..3323)
                     /gene="lpoB"
                     /locus_tag="ACHK1J_000962"
     CDS             complement(2682..3323)
                     /gene="lpoB"
                     /locus_tag="ACHK1J_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO7910275.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3337..3714)
                     /locus_tag="ACHK1J_000963"
     CDS             complement(3337..3714)
                     /locus_tag="ACHK1J_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO7910276.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3717..4076)
                     /gene="hinT"
                     /locus_tag="ACHK1J_000964"
     CDS             complement(3717..4076)
                     /gene="hinT"
                     /locus_tag="ACHK1J_000964"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO7910277.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4410..6599
                     /gene="fhuE"
                     /locus_tag="ACHK1J_000965"
     CDS             4410..6599
                     /gene="fhuE"
                     /locus_tag="ACHK1J_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO7910278.1"
                     /translation="MLSTQFNRDNQHQAITKPSLIAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQTTLNATHSEVE
                     FDSKMMYVDAYINKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGRYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(6659..8092)
                     /gene="ptsG"
                     /locus_tag="ACHK1J_000966"
     CDS             complement(6659..8092)
                     /gene="ptsG"
                     /locus_tag="ACHK1J_000966"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO7910279.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8387..9184)
                     /locus_tag="ACHK1J_000967"
     CDS             complement(8387..9184)
                     /locus_tag="ACHK1J_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7910280.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9195..10199)
                     /gene="holB"
                     /locus_tag="ACHK1J_000968"
     CDS             complement(9195..10199)
                     /gene="holB"
                     /locus_tag="ACHK1J_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO7910281.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYNTLAPEKGKNTLGIDAVREITEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10196..10837)
                     /gene="tmk"
                     /locus_tag="ACHK1J_000969"
     CDS             complement(10196..10837)
                     /gene="tmk"
                     /locus_tag="ACHK1J_000969"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7910282.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRSRYLELAAQDNSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10827..11849)
                     /gene="yceG"
                     /locus_tag="ACHK1J_000970"
     CDS             complement(10827..11849)
                     /gene="yceG"
                     /locus_tag="ACHK1J_000970"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO7910283.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11852..12661)
                     /gene="pabC"
                     /locus_tag="ACHK1J_000971"
     CDS             complement(11852..12661)
                     /gene="pabC"
                     /locus_tag="ACHK1J_000971"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO7910284.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12781..14022)
                     /gene="fabF"
                     /locus_tag="ACHK1J_000972"
     CDS             complement(12781..14022)
                     /gene="fabF"
                     /locus_tag="ACHK1J_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO7910285.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14110..14346)
                     /gene="acpP"
                     /locus_tag="ACHK1J_000973"
     CDS             complement(14110..14346)
                     /gene="acpP"
                     /locus_tag="ACHK1J_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7910286.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14557..15291)
                     /gene="fabG"
                     /locus_tag="ACHK1J_000974"
     CDS             complement(14557..15291)
                     /gene="fabG"
                     /locus_tag="ACHK1J_000974"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO7910287.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15304..16233)
                     /gene="fabD"
                     /locus_tag="ACHK1J_000975"
     CDS             complement(15304..16233)
                     /gene="fabD"
                     /locus_tag="ACHK1J_000975"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO7910288.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WAMTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16249..17202)
                     /gene="fabH"
                     /locus_tag="ACHK1J_000976"
     CDS             complement(16249..17202)
                     /gene="fabH"
                     /locus_tag="ACHK1J_000976"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO7910289.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17270..18340)
                     /gene="plsX"
                     /locus_tag="ACHK1J_000977"
     CDS             complement(17270..18340)
                     /gene="plsX"
                     /locus_tag="ACHK1J_000977"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO7910290.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18421..18594)
                     /gene="rpmF"
                     /locus_tag="ACHK1J_000978"
     CDS             complement(18421..18594)
                     /gene="rpmF"
                     /locus_tag="ACHK1J_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO7910291.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18646..19167)
                     /gene="yceD"
                     /locus_tag="ACHK1J_000979"
     CDS             complement(18646..19167)
                     /gene="yceD"
                     /locus_tag="ACHK1J_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO7910292.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19365..19949
                     /gene="yceF"
                     /locus_tag="ACHK1J_000980"
     CDS             19365..19949
                     /gene="yceF"
                     /locus_tag="ACHK1J_000980"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO7910293.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20061..21020)
                     /gene="rluC"
                     /locus_tag="ACHK1J_000981"
     CDS             complement(20061..21020)
                     /gene="rluC"
                     /locus_tag="ACHK1J_000981"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO7910294.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            21593..24778
                     /gene="rne"
                     /locus_tag="ACHK1J_000982"
     CDS             21593..24778
                     /gene="rne"
                     /locus_tag="ACHK1J_000982"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO7910295.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQTQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24973..25926)
                     /gene="flgL"
                     /locus_tag="ACHK1J_000983"
     CDS             complement(24973..25926)
                     /gene="flgL"
                     /locus_tag="ACHK1J_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO7910296.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPGGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25938..27581)
                     /gene="flgK"
                     /locus_tag="ACHK1J_000984"
     CDS             complement(25938..27581)
                     /gene="flgK"
                     /locus_tag="ACHK1J_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO7910297.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27647..28588)
                     /gene="flgJ"
                     /locus_tag="ACHK1J_000985"
     CDS             complement(27647..28588)
                     /gene="flgJ"
                     /locus_tag="ACHK1J_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO7910298.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28588..29685)
                     /gene="flgI"
                     /locus_tag="ACHK1J_000986"
     CDS             complement(28588..29685)
                     /gene="flgI"
                     /locus_tag="ACHK1J_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO7910299.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29697..30395)
                     /gene="flgH"
                     /locus_tag="ACHK1J_000987"
     CDS             complement(29697..30395)
                     /gene="flgH"
                     /locus_tag="ACHK1J_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO7910300.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30448..31230)
                     /gene="flgG"
                     /locus_tag="ACHK1J_000988"
     CDS             complement(30448..31230)
                     /gene="flgG"
                     /locus_tag="ACHK1J_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO7910301.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31402..32157)
                     /gene="flgF"
                     /locus_tag="ACHK1J_000989"
     CDS             complement(31402..32157)
                     /gene="flgF"
                     /locus_tag="ACHK1J_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO7910302.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGLVLQADPTLRVMSGVLEGSNVNTVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32177..33385)
                     /gene="flgE"
                     /locus_tag="ACHK1J_000990"
     CDS             complement(32177..33385)
                     /gene="flgE"
                     /locus_tag="ACHK1J_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO7910303.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLKFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(33410..34105)
                     /gene="flgD"
                     /locus_tag="ACHK1J_000991"
     CDS             complement(33410..34105)
                     /gene="flgD"
                     /locus_tag="ACHK1J_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO7910304.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34117..34521)
                     /gene="flgC"
                     /locus_tag="ACHK1J_000992"
     CDS             complement(34117..34521)
                     /gene="flgC"
                     /locus_tag="ACHK1J_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO7910305.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34525..34941)
                     /gene="flgB"
                     /locus_tag="ACHK1J_000993"
     CDS             complement(34525..34941)
                     /gene="flgB"
                     /locus_tag="ACHK1J_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO7910306.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTTPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35096..35755
                     /gene="flgA"
                     /locus_tag="ACHK1J_000994"
     CDS             35096..35755
                     /gene="flgA"
                     /locus_tag="ACHK1J_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO7910307.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNASVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35831..36124
                     /gene="flgM"
                     /locus_tag="ACHK1J_000995"
     CDS             35831..36124
                     /gene="flgM"
                     /locus_tag="ACHK1J_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO7910308.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36129..36545
                     /gene="flgN"
                     /locus_tag="ACHK1J_000996"
     CDS             36129..36545
                     /gene="flgN"
                     /locus_tag="ACHK1J_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO7910309.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36585..38120)
                     /gene="murJ"
                     /locus_tag="ACHK1J_000997"
     CDS             complement(36585..38120)
                     /gene="murJ"
                     /locus_tag="ACHK1J_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO7910310.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38230..39153)
                     /locus_tag="ACHK1J_000998"
     CDS             complement(38230..39153)
                     /locus_tag="ACHK1J_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7910311.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSAGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39155..39802)
                     /locus_tag="ACHK1J_000999"
     CDS             complement(39155..39802)
                     /locus_tag="ACHK1J_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO7910312.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39813..40397)
                     /gene="rimJ"
                     /locus_tag="ACHK1J_001000"
     CDS             complement(39813..40397)
                     /gene="rimJ"
                     /locus_tag="ACHK1J_001000"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7910313.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40633..41841
                     /gene="mdtH"
                     /locus_tag="ACHK1J_001001"
     CDS             40633..41841
                     /gene="mdtH"
                     /locus_tag="ACHK1J_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO7910314.1"
                     /translation="MSRVSQARSLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41905..42552
                     /gene="grxB"
                     /locus_tag="ACHK1J_001002"
     CDS             41905..42552
                     /gene="grxB"
                     /locus_tag="ACHK1J_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO7910315.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42686..43246
                     /locus_tag="ACHK1J_001003"
     CDS             42686..43246
                     /locus_tag="ACHK1J_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7910316.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43352..44398
                     /gene="pyrC"
                     /locus_tag="ACHK1J_001004"
     CDS             43352..44398
                     /gene="pyrC"
                     /locus_tag="ACHK1J_001004"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO7910317.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESTCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44472..44717
                     /gene="dinI"
                     /locus_tag="ACHK1J_001005"
     CDS             44472..44717
                     /gene="dinI"
                     /locus_tag="ACHK1J_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO7910318.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10787 a        12116 c        11145 g        10688 t
ORIGIN      
        1 aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgagatc
       61 aaaaaacaga gtttattgtt tattatttga gcgaatctgg aagggtgata tagggtgaag
      121 cgtatatcga gggcgtttgg tcgtactgat aagatgcgca gcatcgcatc aggcattgat
      181 gctcgactgt cggatgcggc gtgaacgcct tatccgactt acagaatccg gagcgcatca
      241 ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt gaacgcttta
      301 atgcgctgca aatgcggaga gatattcttg aatttgtgcg tctgctcttc gtcccagaca
      361 atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagcgc ttcatcatta
      421 cgcgacaaaa tcaccagaca acgatcgcga ttcttctcgc ggaagttggt cacacactta
      481 gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt ctcataaggg
      541 aacaaattag ggttgaagat cacctggcgg atgtcgcaaa gaaaaccaat ccgttccgcc
      601 cagtatccgc ccaagccaac gccgcaaatt agcggacgct cgtcaacgtt cagttgcaac
      661 attttgtcca cttctttaag cagatgctgc atatcatgtt tcggatgccg cgtactgtag
      721 cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc gtggttaccc
      781 ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca taagccaggg
      841 ttagtgacct gctttctctt cctgccagcg ttcatgtaac tgattcagac gggcgctgat
      901 cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt tatgatacaa
      961 acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga cggccccttt
     1021 acgattattg cagaccagga tcatatcgca acccgcatcc agtgatgcct gcccgcgttc
     1081 ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga aaatcacgcc
     1141 gtcaaaaccc agttcctgac gcaaaacggt tttcagccag tagggagaac cgctcgccgg
     1201 acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga gtttgttttc
     1261 gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt gtggacgtgg
     1321 atcgcacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtccag ggaagtgttt
     1381 cccggtcgtt ttcattccgg cttcatgcat accatcaata aaccggctgg caattgccag
     1441 ggctttttgt ggatcggcat gataagaacg ctcgccaatc gccgcgctga tatgcccgac
     1501 atccagcaca ggcgcaaagc tgatatcaat atccatagcg atcatttcgc tggccatcaa
     1561 ccaacctgcc tcctgcgcca gtttgccacc ctcttccatt cctgacagcg cagcgaatga
     1621 ttgcgccgct ggcaagcggg taaaaccttc acgaaaacgc tgcacgcgtc caccttcctg
     1681 atcaaccgcc accaccagat gattgcgcga agctgcgcgg atctggcgca ccagttcacg
     1741 taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca ccagcggatg
     1801 cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca acattactgg
     1861 acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca tcggccagcc
     1921 tgataaattg ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct gctttgagca
     1981 tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga taaatcttcg
     2041 cgcgagtggc atagtcattg accaattgtc ggtgctgttc agcattttcc acccacaccg
     2101 ccgccagttc cagcgcgata tcaccatctc ctgcatactc ccagtcgatg agttttaacc
     2161 ctgacgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga cttaagcgta
     2221 aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcaaccaa cccactgtcc
     2281 gccgcgccgg atcgctttgc tgccagtaca gttccagtaa cggcaacaac gttattcgcc
     2341 agccaaaacg tggttgttga tgtagataat acagcaagcc tgccagttcg ttggtatccg
     2401 gcaaatacgt ttttaccgcg ccgggcagat agtcgactac catccagtca tggagatata
     2461 aatgcggctt cggtgcaatg catgcgggta gttgtgataa agcccgatac tggcgcaaga
     2521 acgctgactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc tggccctggt
     2581 gttcaatgag aaaactcccg ccactaagcc cactattaaa cgtcgtgacg ggatgaaact
     2641 gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattgctg cgaaacggca
     2701 cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat ttgtagggtc
     2761 ggagcgttaa cgttgccaga ggcgctggag tacagcacgt aatgagcgcc gacattgcgg
     2821 gcaatgccta tggctttact acgggtgcct aaactgtcct gcggcgacaa acctaactgt
     2881 tgcttcgcca tcgacagctg ctgggcggaa accagggtaa atttcccgtt attagccagc
     2941 gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt acggttgtta
     3001 acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag catcttactg
     3061 accatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc aggcggtgcc
     3121 gtttgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac cgagggcact
     3181 gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac ttcttctacc
     3241 ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc ggtaatcaag
     3301 gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat aaagtctgac
     3361 tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat
     3421 ggtaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt cataccagta
     3481 aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg aggatgctga
     3541 aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa
     3601 agatgactcc atcaccagcg attgctcatc atttaccgga atttccggat gtgaacgaca
     3661 gcccaccagc agtaacaacg ccagagacac cagcccaaag catccttttc tcatcgttac
     3721 agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg
     3781 tggtaaacct cttgtccgcc atggcggttg gtattcatga tcagacgata accatcttcg
     3841 gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatacgtcc cagcgcctgc
     3901 tcatgctcag ctgacacgtc gttcacagtc ggaatgagga tattcggaat gatcagaata
     3961 tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg
     4021 atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca
     4081 ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat
     4141 agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct taagcaaaaa
     4201 atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa
     4261 agacattctc tgtttggttt acacgtcaaa tatgaatgcg tatatttctc atttgcattt
     4321 acatacaaaa ttattcgcac actaaatata accgctcatc accctgctcc ggcatgagcc
     4381 tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac agggataatc
     4441 aacatcaagc catcaccaaa ccgtcactaa ttgccggttg catagcactg gcactattac
     4501 cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca
     4561 cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa
     4621 tgcagatgac tcaacgtgat attcctcagt cggtcactat tgttagccag cagcggatgg
     4681 aagatcagca gctacaaacg ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa
     4741 gtcaggcgga ttccgatcgt gctctttatt attcccgcgg attccagatc gataactata
     4801 tggttgatgg tatccctacc tattttgaat cgcgctggaa tctgggcgac gcactttctg
     4861 atatggcact ttttgaacgc gtagaagtag tgcgtggcgc gacaggactc atgaccggga
     4921 cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt cgtgaattta
     4981 aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg gcggatttac
     5041 aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata
     5101 acgactcatg gctggaccgc tacaatagtg aaaagacctt cttctccggc attgtcgatg
     5161 ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc attgatgtta
     5221 atagcccaac ctggggcggt ttaccgcgct ggaatactga tggcagcagc aacagttacg
     5281 atcgtgcacg cagtaccgca cctgactggg cgtacaacga taaagagatc aacaaggtct
     5341 ttatgaccct gaagcagcgg tttgctgata cctggcaaac gacgctgaat gccacccact
     5401 ccgaagtcga atttgacagc aaaatgatgt atgtcgatgc ctatataaac aaagcggatg
     5461 gtatgctggt tgggccatac agtaattatg gacctggttt tgattatgtc ggcggcaccg
     5521 gttggaacag cggcaaacgt aaagttgatg cgctggattt gttcgctgac ggtcgttatg
     5581 aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa caaaacaatc
     5641 gttacttcag ttcatgggcc aatattttcc cggatgaaat tggcagtttc tacaacttta
     5701 atggcaattt cccacaaacc gactggtcac cacagagcct ggcgcaggac gataccacac
     5761 atatgaaatc gttatatgct gccactcgtg tcacccttgc cgatccgctg catctgatcc
     5821 tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg gagaaaaacc
     5881 acaccacgcc ttatgctggt ctagtgtttg acatcaatga caactggtcg acctacgcca
     5941 gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc
     6001 caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat agccgtctga
     6061 ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc accggtacac
     6121 ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag
     6181 gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa
     6241 cgcgctatat tgcagaggat aacgaaggaa acgccgttaa tcctaatctg ccacgcacca
     6301 cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg
     6361 gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc accttccgcg
     6421 ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg acgaaaaact
     6481 tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc aacgtggaag
     6541 gttctatcgt ctacggcaca ccgcgtaatt tcagcattac cggcacgtat caattctgaa
     6601 taacacctgt aaaaaaggca gccatctggc tgccttagtc tccccaacgt cttacggatt
     6661 agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta gtaccgaaaa
     6721 tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc ttcaggccgg
     6781 cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata catgcgtcga
     6841 ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc atttcgctgg
     6901 tacctgtcgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc agatccagtg
     6961 ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata ccgacgatcg
     7021 ggaacagcca cagtttgctg ctgttaccag acagaacgat gaagtcgatc agaccgtgcg
     7081 agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc aggcctgcca
     7141 gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaaggagaac tcgatcggct
     7201 cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg cccactttcg
     7261 cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc agaccgtaca
     7321 ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc atataacgcg
     7381 gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc tgcatctgga
     7441 aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt
     7501 aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag gtctggattg
     7561 cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg gcagccaggc
     7621 cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca ggcagcttaa
     7681 tacggtagaa acggttaaac atgtacgctg cgatcgcacc ggagataatc cctccgagta
     7741 cgccagtatc cgccaggtgt ttagaggcga tttcttcagc aggtaaatgc agtaccagtg
     7801 gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg gccagcgcgg
     7861 atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt
     7921 ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg ggcagccagc
     7981 tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggcagtacg gataccggca
     8041 gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac ataattgaga
     8101 gtgctcctga gtatgggtgc tttttttacg ttctcacgcg tggcaagggg ggagagcctc
     8161 gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat aattcagtgc
     8221 caatttgttt gacggctatc acgtttcaac ttcagatcat tggagaagtt gcacattttg
     8281 acaaaacata tgtcacaata tttcaaacgt ggttattcac cgcccaattt tcggcggtga
     8341 actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa cggatggatt
     8401 gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt acctgcgcca
     8461 gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg
     8521 caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca
     8581 ccagtaaccg atccaggggg acataacgcg cagcatcgcg cagttgctcc gcattacgga
     8641 aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta cccgccgttt
     8701 ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca
     8761 gaattgccag cgtatcggca cgggcgtcac gggtatggac gataaccggc ttgttcagtt
     8821 cacgaccaat ctggatatga tggatgaagg actcttgctg acgtacttta gtttccggtg
     8881 tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct gccgccagac
     8941 ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg ccacaagaaa
     9001 atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc ggtaatgttg
     9061 tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca
     9121 cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag tcgactaaaa
     9181 acatgatgtc tctcttaaag atgaggaacc ggtagcacaa cgcccggttg caggtaatgc
     9241 tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt gacagacatt
     9301 aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcgcga gggagaaaga
     9361 tggtttgcca gttcgacgac caggcccggc acatcaacat tggtcacctg cgcagcgcca
     9421 tgatggcgtt ttagcgcatc catcagcaac gttgccagcc agtgtaaacg cgccggagct
     9481 tgttcatgat taagggccgc tagcagcgaa taccagtcgc ccgatggcac gctatatgcc
     9541 aacgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa cgccagtgcc
     9601 gcgccaggcg aaccggcgct taaacgcaat gcggcaagta atgcatcctg tgacattgtc
     9661 acttcgcgtg aaagccaggt cacggcgtac tgttccggcg gcggcgcaag gtaatgtaac
     9721 cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac
     9781 caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc
     9841 agtaaggcag catcggttac ccagacaact ttcgcaccac ctaagcgtgc gtgctcattc
     9901 agcttttcgg tgatctcacg taccgcatca atgcccagcg tatttttccc tttttcggga
     9961 gccagggtgt tgtaatcggg atgcgtgcca gcctgcatca actgacatcc acgacagtga
    10021 ccgcaacttt tgtggccctg cggttgttgg cagagtaaat aacggctcag ggcgtagatt
    10081 aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg acctcttccg
    10141 gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata ccatctcatg
    10201 cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc acggcctcca
    10261 gcggctgggt ggcatcaatg gtatgaatac tgttatcttg tgcagccagt tccagatagc
    10321 gggagcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc agctcgccgc
    10381 gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc gttaagtcgg
    10441 ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg tcaataccac
    10501 gtccgccgcc ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc acccaggtgc
    10561 cgttagccag cgcaggtttg atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca
    10621 tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata tccagcacca
    10681 ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgcgtgaaa accatgtcgc
    10741 ggatacccag ttgctcaagc gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc
    10801 cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag cactttcaga
    10861 taatcctgca cagacttgtt atgacttgca agattggtat taaacgtgtg accaccttta
    10921 ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc cttcagcgaa
    10981 tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata ggtgttatac
    11041 gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc tcccatcccg
    11101 taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc
    11161 gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat tgatgccatc
    11221 gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc ccaggcgcta
    11281 tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta acgcgacatc ggtggtattg
    11341 gcggtataca tccaggtgtc tggccagaac caaccttcaa tccactccgg gttttccagt
    11401 tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt gatatacgga
    11461 gcctcgcgca attgcttgag gtagtcgctc agacgcatcc cttctaccag tcgcagcggg
    11521 aactgtgctt ctttaccgct ttccaacaat ttcagcatct cgcgcacggt catctgcggt
    11581 gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg cagcaaccat
    11641 tgaaaaaccc gtgggcgatt gatgatctta tcggcataaa gctgttcacc aagcgccaga
    11701 cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg
    11761 gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac caccagcaat
    11821 aacaagataa tcaataacac ttttttcatg actaattcgg gcgctcacaa agtggggcta
    11881 aatattcata taacgttgct gacgaaaagg agacatcgcc acaggcacgt acgggcatca
    11941 ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctct tccagagagg
    12001 cttgcacttc gacaagctga taagaggatt gtgccagtaa acggatacag aattgtcgca
    12061 taatgccgtt aacacctgcc tgatccagtc gcggcgtata aactacgttg cccttccgcc
    12121 agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg accagcgcct
    12181 catcagcgtt tgtctgctca agatgagagc gaatcaatac ttgctcaaga cgattgagat
    12241 gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc gccaacgtca
    12301 tcccctcgtt acgcaaacgg tcgtaatgtg caggataagc cgtaacggag agaatccgcg
    12361 ttgccggtcc actgttcaat gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga
    12421 ctttcagtac accattttgc tgttctgctg ccagcgtttt catctcttgt tcaagctgtg
    12481 gccagaagtc acaggaaatc atcaaccgct gacaagcatc ctgtagtcgc tggatatgcg
    12541 ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa
    12601 actgcgttgc ccgatcgctt actgccagcg attcctgctt ataaccgtta attaagaaca
    12661 taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg aacaagtcgg
    12721 aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtgga aaatgacaac
    12781 ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg agttacacag
    12841 cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc
    12901 ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca gcgccaggat
    12961 ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca tagatttcgt
    13021 ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg cctgcgcttc
    13081 agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc
    13141 ttcaatgcct gcatcacgca gagcatttgc cattgccaga gctgcgcctg cgccattttc
    13201 tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga gttcagcgta
    13261 aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca taccggcacc
    13321 atcgcccagt acgaagccat cacgctcttt atcccacggg cggctcgccg cttgcgggtt
    13381 atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacgccca gcggcgtact
    13441 ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga taatacgcgc
    13501 agcatggcca atgttgtgca cgccggaagt acaggcggtc gcgatagaga tgcttgggcc
    13561 acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg ttgacggaac
    13621 gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt tttcttcgat
    13681 cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg cgttctcttc
    13741 cgttatttca aggccagaat cctgcatggc ctgaacgcca gcgacaattc catattgaat
    13801 gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagt taaaatcctt
    13861 tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga ttaggctgat
    13921 gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc cgacaggaga
    13981 caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc tccagggagg
    14041 gaaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg accgcctgga
    14101 gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat
    14161 tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag ccattaccag
    14221 ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac
    14281 ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt cttcgatagt
    14341 gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg tagtgtataa
    14401 aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta cgttattttg
    14461 aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat aaccacgcag
    14521 actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt
    14581 cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc
    14641 gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca ggatacccgc
    14701 acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg gagcaacaac
    14761 gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga agccgatcaa
    14821 gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac caaccacaga
    14881 accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta ccgcttttga
    14941 cagacggaaa acagatgaaa ggttggtttc gataatatcg ttccactctt catctttcat
    15001 tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat ccacttcacc
    15061 aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg tcacattcaa
    15121 catcagacct ttgccgttag cacctaaata atcactgatc gcctgagcgc cattttcact
    15181 ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc
    15241 aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca taattttcct
    15301 cttttaaagc tcgagcgccg ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag
    15361 ggtgtcgaca atgcgtttcg tcaggccagt aagcactttg cccgggccga cttcatagag
    15421 atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa ccgggttata
    15481 caactgacgt accagtgcgt cacggatggc atcaccattg gtttcgcatt tcacatcaac
    15541 gttattcaca acaggaactg ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag
    15601 tttgtcggct gctggtttca tcagcgcaca gtgagatggt acgctcactg gtaacggcag
    15661 agcgcgtttt gcgcccgcag ctttacaggc agcgccagca cgctcaaccg cttctttatg
    15721 accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga cctgaccttc
    15781 tgcagcttct tcacacgctt tcgcaataga cgcatcatcc agaccgatga ttgccgccat
    15841 agcgcccgtt ccttccggta cggcttcttg catgaacttg ccgcgcatct caaccagacg
    15901 caccgcatca gcgaaatcaa tcacaccagc gcaaaccagc gcggagtatt cccccaggct
    15961 gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc ggtacagcgc
    16021 aacagaggca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt cttcagctgg
    16081 cccctgctgg gtcatcgccc acaggtcgta gcccagcgcc gcagaagctt ctgcaaacgt
    16141 ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg tttgagaacc
    16201 ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct agaaacgaac
    16261 cagcgcggag ccccaggtga atccaccgcc aaaggcttca agcagaacca actgccccgg
    16321 cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg ccgcagaggt
    16381 attaccgtgg cgatccagcg tcaccacgac attgtccatc gacataccga gttttttcgc
    16441 cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca gttgagaacg
    16501 gtcaagatta ttcgccgcca gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac
    16561 cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat tcacgcggtc
    16621 ggcgtttggc agcgtcagca attcaccata actaccgtcg gcatgcagat gggtggagat
    16681 gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc caaaaataat
    16741 aatagtccca cgatctgttg gatcgcaggt gcgcgccagt acatcggaac cgacgaccag
    16801 agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg cataggtgaa
    16861 acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgccca acatgctttg
    16921 aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag
    16981 gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc
    17041 catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt
    17101 gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg tccgcacttg
    17161 ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc agtcactttt
    17221 cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc taccgcagag
    17281 ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat tccaggcgag
    17341 cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg
    17401 caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc aatcctaaca
    17461 gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc aggctctttt
    17521 gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca ccctgagatt
    17581 tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaatgtg acatttcctg
    17641 taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg gcttcaagat
    17701 agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc cgaatactgt
    17761 cgagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga ttgggaattt
    17821 ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac attgtgctgt
    17881 cacaatcgac gttggcccct aaatcgagga ccaccgtttt gcccttttgc tgatgcggta
    17941 ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat aattttgcca
    18001 gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga ccttctttca
    18061 ccagctccag ggccacgcgc attgaactcc cacgactggc gcggatagct tgcgaaggcc
    18121 gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa cgttgttcaa
    18181 agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga
    18241 gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat
    18301 cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt
    18361 tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga
    18421 ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag
    18481 gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca gcgcgtcatg
    18541 ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg ccatggacct
    18601 tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact tacgctttaa
    18661 gctggctaat acggcaaatg ggtttggctt ttgcgcttct tcaggcagtt caccaaagac
    18721 catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta ccggcaaggc
    18781 gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt cgttaacctc
    18841 aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca caggactaaa
    18901 acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac actcgagcgt
    18961 taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga tagcgaacga
    19021 catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc gctcaacctg
    19081 atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag
    19141 agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta acgtcataga
    19201 gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac attgcgggtt
    19261 actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg aagcgcatag
    19321 tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaatatgcct aaacttattt
    19381 tagcctccac ctcgccctgg cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat
    19441 gtgcagcacc tgaggtcgac gaaaccccac gcagcgacga atcaccgcga cagttggtgc
    19501 ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat catttaatta
    19561 ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag
    19621 aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc tatactggac
    19681 tggcgttgtt taattcggcg aatgggcatc tgcaaacaga agtggagcct tttgacgtcc
    19741 atttccgtca cctgagcgag gcggagattg ataattacgt gcgtaaggag catcccctgc
    19801 actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag cgtttagagg
    19861 ggcgtgaccc taacacgctg gttggtttac cgctaatcgc gctgtgtcag atgttacgtc
    19921 gggaagggaa aaatccgctg atgggatagt tttttgcgcc agatgcggtt acgtcgcatc
    19981 cggcaatcgg tgctggcggc ctgatgcggc gtaaacgcct tatcaggcca accgcccatt
    20041 atcaggctta tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa
    20101 ccatcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg ggtaaacttc
    20161 aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt gagctgtctg
    20221 tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc atactgtgta
    20281 tgcacacgga tctgatgagt acgtccggtt accgggctac agcgcaccag ggtggcaaat
    20341 gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg actcacacgc
    20401 acgatacgtt cgccgctttg cagaatattt ttcagtaacg gcgcttgaac gctcttcaca
    20461 tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcatccc tttttcacgt
    20521 aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa tacacctgaa
    20581 gtgtcccggt cgagacgatg aaccagttca aggaaccgcg cttccgggcg caacgcccgc
    20641 aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt accggaaggt
    20701 ttattcagca ccaggatatg atcatcttca tataagatga cgtccgccag cgccgccacc
    20761 ttttgcagat gtggtgaaac cgcctcttct tcccgctcag caacgcgaac cggtgggata
    20821 cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt gttcacccgc
    20881 acttcgcctt tacgcaaaat acggtaaatc atactttttg gtacgccttt caattgggta
    20941 cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac aatttttacg
    21001 gatggagtct ctgttttcat ggttggcgat tctaattagc caacaggatt cgcgccactc
    21061 atttttctat gcttatattt acttttgcac cttattactt cactgcgtgc tcactttatt
    21121 gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa
    21181 atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg
    21241 tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga ccgatcatcc
    21301 acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg cgggttgcag
    21361 tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt ctttgataat
    21421 tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt tgagctgacg
    21481 ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac ccggaaaggc
    21541 gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta cgatgaaaag
    21601 aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg
    21661 tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa acatctacaa
    21721 aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt acggcgctga
    21781 acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg ctaactacag
    21841 tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggagg tcattgttca
    21901 gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta tcagtctggc
    21961 gggtagctat ctggttctga tgccgaacaa cccgcgcgcg ggtggcattt ctcgccgtat
    22021 cgaaggcgac gaccgtaccg aattaaaaga agcactggca agccttgaac tgccggaagg
    22081 catggggctt atcgtgcgca ccgctggcgt cggcaaatct gctgaggcgc tgcaatggga
    22141 tttaagcttc cgtctgaaac actgggaagc catcaaaaaa gccgctgaaa gccgcccggc
    22201 cccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg attacttacg
    22261 tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg cacgtcagca
    22321 tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca ccggcgagat
    22381 cccgctgttc agccactacc agatcgagtc gcagatcgag tccgccttcc agcgtgaagt
    22441 ccgtctgcca tccggtggtt cgattgttat cgacagcacc gaagcgttaa cagccatcga
    22501 catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa
    22561 cctcgaagcc gccgatgaga ttgctcgtca gctgcgcctg cgtgacctag gcggcctgat
    22621 tgttatcgac ttcatcgaca tgacgcctgt acgccaccag cgtgcggtag aaaaccgtct
    22681 gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt ctcgctttgg
    22741 cctgctggaa atgtcccgtc agcgcctgag cccatcactg ggtgaatcca gtcatcacgt
    22801 ttgtccgcgt tgttctggta ctggtaccgt gcgtgacaac gaatcgctgt cgctctctat
    22861 tctgcgtctg atcgaagaag aagcgctgaa agagaacacc caggaagttc acgccattgt
    22921 tcctgtgcca atcgcttctt acctgctgaa tgaaaaacgt tctgcggtaa atgccattga
    22981 aactcgtcag gacggtgtgc gctgtgtaat tgtgccaaac gatcagatgg aaaccccgca
    23041 ctaccacgtg ctgcgcgtgc gtaaagggga agaaacccca accttaagct acatgctgcc
    23101 gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg
    23161 tccggaacaa cctgcgctgg caacctttgc catgccggat gtgccgccag cgccaacgcc
    23221 agctgaacct gccgcgcctg ttgtagcccc agcaccgaaa gctgcaccgg caacaccagc
    23281 aactcctgca caacctgggc tgttgagccg cttcttcggc gcactgaaag cgctgttcag
    23341 cggtggtgaa gaaaccaaac cgaccgagca accagcaccg aaagcagaag cgaaaccgga
    23401 acgtcaacag gatcgtcgca agcctcgtca gaacaaccgc cgtgaccgta atgagcgccg
    23461 cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg
    23521 tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcagg ctgaggtaac
    23581 ggaaaaagca cgtaccaccg acgagcaaca agcgccgcgt cgtgaacgta gccgccgccg
    23641 taacgatgat aaacgtcaga cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc
    23701 tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg
    23761 tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg tagttgcacc
    23821 ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagcgccag ctccacgcac
    23881 agaactggtg aaagtcccgc taccagtcgt agcgcaaact gcaccagaac agcaagaaga
    23941 gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg
    24001 tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca
    24061 atcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg
    24121 gatccgctat ccgattgtac gtccacaaga tgtacaggtt gaagagcagc gcgaacagga
    24181 agaagtgcaa gtgcagccga tggtgactga ggtgcctgtc gccgccgctg tcgaaccggt
    24241 tgttagcgcg ccagttgttg aagaagtggc tgaagtcgta gaagcccccg ttcagattgc
    24301 cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtaattgccg ccgcggtaac
    24361 tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag tcgcagaaca
    24421 cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag ttgccgaaac
    24481 tgctgaggtt gtagttgctg aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc
    24541 tgaagtcgca gcagaagttg aaacggtaac cgcggttaaa ccagagatca ccgttgagca
    24601 taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc
    24661 gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg
    24721 tcatacggca acacatcatg cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata
    24781 attagctcaa gtaatcaagc cctggtaact gccagggctt ttttatttca tctttgaatc
    24841 aacatattaa aattcatcga atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa
    24901 aatgcccgcc ccggcgatcc cagagcaggc agacaaaaca tacccagttt catgatatgt
    24961 tttaaagcga aattatttgt tgagctggaa gagcgacaat ccctgcatat cggtaaatgc
    25021 tttatacgat gcctgcaatg ccgtttgctg catgatgtaa gatgaaatag ttgcattcca
    25081 gtccacatca accagatcgc tcatctgctg cgtttgccct aaagcgcgat cgctacctaa
    25141 tgaatccagc gactccagtt cgttcagctg cgtgcctaat tccgcgcgaa cagtcagcac
    25201 attgttcagc gagtttttca gtccgcggtt ggttttatcc agtgccgcag cggcggtttc
    25261 tttatccgct tcgctatccg cgaccggcgt tttcagggct gcgatggcac tatccagcat
    25321 ggcaaaaaga ttggtttcag aagcgctacc gcctggttcc gctaccgcgt tgctggtaat
    25381 actgtcgaaa attttgtcac ccgtgtgccc tattaccatc gaacgcgaag catcgacctg
    25441 ttgtttaata ctttctgttc cgccgacata atcaccgtta gcctcactaa acggcgcagt
    25501 ctctgtttta taaccggcaa aaatgtagcg cccgttaccg tcagtggtgt tcgccagatt
    25561 cagcaactgg tcacgaagcc cctgaatatc cgtagccagc gaggcccggt catcgtcact
    25621 caaagtgcca ttgctggcgt agacaatttt ttcctgagca ttctggatag ctgtggtgac
    25681 ctggctaagt acactctctt ccagtgacac tttttgagtg gcgaaagtac gcgccagcgt
    25741 gtactggctg ttttgcgcct gtgcctggga gagaactacg gcttgtgatg ctgcaatggg
    25801 atcgtcagaa gggttaacga ctcgcttacc tgtcgacatc tgctcgccgt acttcatcca
    25861 ttccgcctga gaattggtga tgccacgcat gttttgctgg tacatcatct gggtactgaa
    25921 acgcatctta tctcccctta gcgaatgtta atcagcgcat caaaaatcgc gtttgccgtc
    25981 tgcagaacct gcgcattcgc caggtaatac tgctgaaaac gttgcagatt tccgtactcc
    26041 tcatcgagat tgacaccgga aatcgactgc tgctgattgg aaagctgcgt caccacatta
    26101 ccttgcgtgg tgctactggt tttcaacgtc gcggttttat taccgatatc actcactaac
    26161 gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg ttttactgtt gctttgcaga
    26221 tccagcaggg cctgaccgtt gcggttgtcg ctatcacccg catcttcttc gctcgccatc
    26281 gcgattttcg cttcgtcggt gattaataca tccatgttga cgatggcgtc acttactggt
    26341 ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa acgtcaactc cagaccatca
    26401 aatgccactt taccgttggc atccggcgtc accgtaaaag tggtattgct ggcaaggcgg
    26461 gtgacctgcc actgattatt atcgaacgag attttgtaat ctgtcgccag taccgcggag
    26521 gcatcagtta ccgtggcacc gatcgcaacg ttaccgttgt ttttcgtgtt ttgcagaacc
    26581 gcgggcttac cgatagcaaa gaaatcttca ccctcatcgc cgttggcatc aaatccggct
    26641 ttgtgttggg tgttgaaagc ctcagcaaat gccagcgcca gttgtccaag cgtattacgc
    26701 gtctggtcca gatcctgaga acggaatgtc agaatgccgc ccagcgaccc ggtattcagt
    26761 aatttctccg ggatctcaat attgcctgcc gtcccatcaa cataagcgac agtcgtacga
    26821 gaagggtcgg cgctggaagg aactgccgcc aattgccgcg ccgtacttcc ctgaaccagt
    26881 gagtaaccat tggccatcgt gatgttataa gtgccgccat cctgaacgct gacttctaca
    26941 ccaacaatct ggtttaattc gctcaccagt tgatcgcgtt gatccagcag attgttaggt
    27001 gacgcccctg cccccacgcc tgtcaggcgg gagatttgat cgttcaggct ggcgatttgt
    27061 ttagcgtaat tgttgatctg atcaacgctg gcacctatcg cgatattgac ctgtttgtcc
    27121 tggtcgcgaa gatactgatc ggtggtttta aactgattca ccaacccttc tgatttccca
    27181 atcagcgcct ggcgcgctgc cgggtcttcc gcgttactca ccagcgtttg caggctggtg
    27241 aagaaatcct gcatctgtgt tgccagcgaa gaggtactgg tggagagcat attgtcgatt
    27301 ttcgacatct gctcatagcg ggcagtcagg ccgctacttt gcgtctgcgc cgcacgtaac
    27361 tggttggtaa taaatgcatc atactcacgc tgcacaccag aaacgtagac gccattgcca
    27421 acccagccgc cagcgcccaa cgtgctattg gcctgcgcca taatagtagt ttggcgggta
    27481 tatccggcaa cgttatagct ggagatatta ttacttgctg tatttaacgc cgcctgggcc
    27541 gcgttcagtc cgctcatggc gttattgatc aagctggaca tggaggttcc ttataagcct
    27601 tcaattacag agtattatcg gcagcgcccc gccggacttg agttattcag aacagattat
    27661 caatgttcat actgtaggtt ttgctcacct tgtcgcttat cgatttcatc tgctgaatca
    27721 tgttggtgag tttacgggca tagtgaggat cggtggcata gcccgcgtcc tgtagggcct
    27781 gcgccccctg ctccgcactc gcggcggtcg tcacggcggc gtagcgcggg ttacgtgtta
    27841 acagcccaac gtaatccgac aatgcttcca gatacgagct gtagacccga aacttcgctt
    27901 ttactttctt cgcttcgcca ttttcatatt cagtcgtggt gatttcagtg actggccctt
    27961 tccagttgcc agaggctttg acaccaaaca ggttatagct cggctcgccg ttttcgcggc
    28021 ggatttgccg ttgcccccag ccagattcca gcgccgcctg agcgaggatc aaatgatgtg
    28081 gcacaccgct ttgctggctt gccagttggg cgggcagcga gagttgcgcg aggaatgctt
    28141 tactgtcacc tggcagcgaa tcatcgtagt tacgtggcac ggccttttgc accagctgcg
    28201 aaagcgcctg attttgataa cgcaccacgg tttcgagcgg gaatttcatc ggtgctgctg
    28261 gcgtggactc ctctggcaat ggttgttctg gcgtcatctg cttaaccatc atctctgcaa
    28321 gccccagacc tttgcccgcc gtcatctgtt gggcaatctg ctggtcatac atactggtat
    28381 acaggcgagt gtgctcgctg ctgaacaggc catcttttgg taaagcgtcg cgcatgcttt
    28441 tcaacatcat ctgcacgaac atcccttcca cctgacgggc caccggacgg atatttgccg
    28501 ccgggtcttc gcccgctttc gcctttagtt cgttgagtga ttgcgcatcc caggccgcac
    28561 ttgccagtag tttgctgtcg ctgatcatca gatgatttcc agttttgccc gcagacatcc
    28621 cgcactttgc attgattgca gtatggacat taaatccatt ggggtggctc ccaaggcgtt
    28681 gagcgcgcgc accacgttat tgaggctggc gctggaacgt acgctttgca gcgaaccgcc
    28741 gctctggcgt aaatcgatct gcgtttgtgg agtaaccaca gtctgtccac caccaaacgg
    28801 tgtatctggc tggctgacat tggcctgacg attgactgta actgagagat tcccctgcgc
    28861 taccgcgcag ctgtcgaggg tcacttcgcg attcatcacc accgaaccgg tgcgcgagtt
    28921 aatcactact ttagcgtcct gcggggtgac attaacctgc atattctgga tatcggcaag
    28981 gaaacggacc tgggagctgt tgccacttgg tacgcgcacc tgaatagtcc gcgcatccaa
    29041 cgcggtggcg ctgccatatc cacgcactcg gttgatggtg tcagcgattt gctgcgccat
    29101 gctgaaatct tcgtcgttaa gttgcaaatt aagggtattc ccgacgccaa actggctggg
    29161 taattcacgt tcaataactg caccattggt gatccgtcca ccgttcagtt ggttcacctg
    29221 aacactgcta ccgccagcgg aggctccagc gccgccaacc agaatattgc cctgtgccag
    29281 cgcatacacc tgactgtcaa cgcccttaag cggtgtcatc aacaacgtac ctccacgcaa
    29341 gcttttggca tttcccatgg aagaaaccac cacatcgatg gtttgcccct gacgtccaaa
    29401 cggaggaagt gacgctgtca ccattaccgc agcgacgttt tttagctgca tattggtgcc
    29461 cgtcggaacg gtaattccca gctgtgagag catgttatta agcgtttgtg tggtaaacgg
    29521 cgtctgggtt gtctggtcac cggtgccatc cagccccacc accagaccat agccaatcag
    29581 tgagttttgc cttaccccct gaacactggt gagatcgcga atacgctcag cctgagccgc
    29641 cgtcgtaacc agtagaagaa ttaatgcaga gagaaattta atcaccacag cctcacttac
    29701 attggcgaca ggttaaggaa gaaacgctgc aaccagccca tattttgcgc ttcgttaatg
    29761 tagccattgc ctacgtattc aatgcgcgca tccgccacct gagtagacgg tacggtattg
    29821 ctgccgctga tggtgcgtgg attaaccacg ccagagaagc gaataaattc ggtaccctga
    29881 ttaatggcaa tctgtttttc acccaccaca tgcaggttgc cgttgaccag tacctggtca
    29941 accgtcaccg tcaacgtgcc gctaaaggta ttgctggcat tggccccgcc ctttccgttg
    30001 aacgtgttac caccggaggc ttcgacatcg gcacgagcgt taccaaacag cccctgcaaa
    30061 tagcgcggca cagtatcaaa gccaaaatta gttttaccgt cacggctggc attcgcagag
    30121 gagcttttgc tggcgctgac gttctcctgc aacacgatgg tcagcgtatc gccaatattg
    30181 cgtggtcgac gatcttcaaa cagcggttga tagccatagt taatcggctg agcagactgg
    30241 aaaatagaac cgttggcgac gggcgtcgga ccgggaaccg gttgtgcact ggtcgccccc
    30301 tgcaccagcg gcgtggaggg tatccaggcg cagccggtta gtgaaagcac caacaagctg
    30361 gaaatggcat aagtatgcgc agcgtttttt tgcatggcta tcatcttcaa aaatcaataa
    30421 accggtgaac ctgccaccgg ttatgcctta gagttgcgtc agtttttgca gcatctgatc
    30481 ggtggtggac accgctttac tgttgatttc gtaagcgcgt tgcacctgaa tcatattgac
    30541 cagttcttcc gccacattga cgttagacgt ttcaacatac ccttgataca gcagtcccgc
    30601 gccgttcagg cccggcgtgc tttcgttcgg tgcaccggag gattgcgttt cggtgtagag
    30661 gttttcgcca atgctctcca gcccggtgtc attcataaag gtggtgagat tgagctgccc
    30721 aacctgaacc ggagctgcct ggccttgttg ggttacgctg accacgccat cacgaccgat
    30781 ggtgatactt aacgcattcg ccggaatggt gatcgctggc tgcacctgaa aaccaccagc
    30841 cgtcaccagc tgcccgttct gatccacctg gaaagagccg tcacgggtat aggctgatga
    30901 gccatctggc aacatcacct ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt
    30961 gttggtctgc gacaggttgc cctggctgtg taagcgttca gtggcgaccg ggcgtacccc
    31021 cgtgccgatt tgtaatccgg agggtaaggt ggtttgttcg gaagactgtg cccccggctg
    31081 gcgaatggtt tgataaagca gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt
    31141 actgacgttt gccaggttgt tggcaatgac gtccatattg gtttgctggg cgtcaaggcc
    31201 cgttttggcg atccataatg aactgatcat aaaatgtcct gtagtggatt agtgacaaag
    31261 tgcatatgtc ttcttgccgg atgcggcgta aacgccttat ccggcctaca agtgctcgca
    31321 aaatcgataa attgcgattg cccccataga cccgataaac ttgcgcatcg ggcaacttat
    31381 acttgtcatg tatcctttca attaactcat cgacagcagt tggttggcac ggcctgcgtt
    31441 atcatcgacg ctgctgatca ccttcatctg catttcaaaa cgccgcgcgc tggcaatcat
    31501 gtcgctcatt gccgcaacgg tattgacatt actgccttcc agaacacccg acatcacacg
    31561 caaggttgga tctgcctgca gtacaagccc acgcgtggcc tgggtttctg cgcttaaacg
    31621 aaaaatgccg tcgtcaccgc gctgcacttc gctgcccgtg gctttcacca gtttaagacg
    31681 ccctactggc gcaaccgtat ttgccggatc gcccggattc agcgccgaga ttgtgccatc
    31741 ggcagcaata gtgatttccg ccccttccgg cacagcaatt ggccccgcct cgcctatcac
    31801 cggatgcccc tgaattgtca gttgcccggt gggatcaacc tgaatgctgc cattacgcgt
    31861 atacccttcg ctgccgtcag cggtctgcac ggccagccag ccatcctgct gcaatgcaac
    31921 gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc atgtctgcac ccggtgttga
    31981 cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc accggcactg cgcgcaaggc
    32041 gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc agattactgg cggttaccgc
    32101 ctgttgattc agcgtctggc tggctgctcc catcgcggta taaattgcgt gatccattga
    32161 gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt gaggatctgg tcctgggttt
    32221 tgatggtctg ggcgttagac tgatagttac gctgggcaac gatcatattg accagttctt
    32281 tactgagatc gacgttggac gcttccagcg caccgttggt cagggtgcca aagtttcccg
    32341 tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag acgttgtcgc
    32401 cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc cccagcagtt
    32461 gggtttgttc attggaatag ttgccgacaa ccgtaccgtc atcattgatt tgataactca
    32521 ccagatcgcc cggtttgtag ccattctggg tggttgccac aatgttgtta gcgccggtat
    32581 tttgctgcat ggagttgagg aagctcaggc taaacgtggc gggttctgca ccgttaattg
    32641 cgccggttgc gatattattc gccatcgcac catccactaa tgtgccatta gcattaaatt
    32701 tcagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg gtgtagacct
    32761 gccagttatt atccccggtc ttcacaaagt acacgctcat gtcatgagca ttaccctgac
    32821 tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcatcaaatg
    32881 cgttaacaga gggaagcgga tcgctggaat tcaggttgat ctgcatcgac gccgtggtgg
    32941 tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc gccccttgct
    33001 gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct tgcatattca
    33061 ccaggttacg attttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt
    33121 tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg
    33181 tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt acccccagtc
    33241 ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtatg
    33301 tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca gcgtttaatc
    33361 cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct
    33421 gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgctgt
    33481 tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac
    33541 caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg ctgccatcag
    33601 tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg
    33661 tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt
    33721 gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg
    33781 caagaacagt ggtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg
    33841 actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt ttttcaatcc
    33901 cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg
    33961 tcgggtcctg gtttttcagc tgcgccacca acaaagtcag aaaactgctt tgtaaatctg
    34021 cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat
    34081 cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt
    34141 cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc
    34201 cgacatggtg ttaaccatct ctccgacaac atcaacgttc ggcattttta cgtagccctt
    34261 tgcatccgcc agcggattac ccggttcata aaccagtttg tccggggcct gactttctat
    34321 aacatcggca acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctgaaatac
    34381 cacctgtttt gcccgatatg gctgtccatc gggaccggtc acgctatcag cattcgccag
    34441 attactggcc gccacgttca ggcgctggga ctgggcagtt aacgccgacc cggcgatatc
    34501 gaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt
    34561 gatttgcccg ctcaacgcac taaggctcat ctggtattgc aggctgttat cggcaaactg
    34621 ggtgcgttcg cgatccatat cgacggtatt accgtcaagc gaaggctggt ccggaatacg
    34681 gtattgcagt tctgcggtag gagtcgtcag cgcctgcgcc ggaatgtgtt gcgttgaggt
    34741 catcgtcagt gcaaccacac tggttgcatc ccgtccacgt tgcatgactt ttttaagttc
    34801 actggcaaaa tcgatatcgc gcgcctgata accaggggta tcggcattgg cgatgtttgc
    34861 tgccagcact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc
    34921 ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattctgcc atcagcttaa
    34981 atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt
    35041 tgatggtcat tgcggacagg tacaattcac gttgtagaaa tggctggggg cgaaaatgct
    35101 ggcaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa gtgcggcgag
    35161 caatctcaca tcgcaattgc aaaccttttt tagctcccag ctcgcaggga taagtgatga
    35221 ggttcgtgtt tctattcgta cagcgcccaa tctactaccg ccgtgcgagc agccattgct
    35281 ttcgatgagc aataattccc gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa
    35341 cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg ttgccgcgat
    35401 gcccattgtg cggggaggaa agctggaagc tggcaatgtc aaactaaaac gcggacggct
    35461 ggataccctg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg ccgttagcct
    35521 gcgcgatcta tcacccgatc aacctatcca gttaacccag tttcgccagg catggcgggt
    35581 aaaagcggga caacgcgtca atgtgatcgc cagcggtgat gggtttagcg ccaacgctga
    35641 aggtcaggcg ctgaacaatg catccgtcgc acagaatgcg cgggtgcgca tggtatctgg
    35701 acaggtagtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc tgtaagctgt
    35761 taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg ccctcaatga
    35821 ggaataaacc atgagtattg atcgcacttc gcctctgaag cccgtaagca ccgttcaacc
    35881 gcgcgaaacc actgacgcgc cggtaacgaa cacccgggcg gcaaaaacaa cagcatccac
    35941 cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga
    36001 tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat
    36061 ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact tgcagagtaa
    36121 ttaatcgtat gacgcgtctt gcagagatcc tcgaccagat gtccgctgtg cttaacgatc
    36181 tcaaaacggt aatggatcaa gagcagcaac atctctctat ggggcagatc aacggcagcc
    36241 agttgcaatg gattacagaa caaaaaagct cactgctggc aacgttggat tacctcgaac
    36301 agttacgcag gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg
    36361 aaattgctgt gaaaacgcag caactacgcc aaatgaatca acataacggc tggttactgg
    36421 aaggacagat tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga
    36481 cgctatatgg ggcgaacggt cagacctcaa caacccatcg cggcagtaaa aagatttcga
    36541 tctgaagatt atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg
    36601 gcaaattctt taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc
    36661 gccagcacga ccgccattaa acgcagcaag cgccagggca tggtacccaa tgaccactcc
    36721 ggcatgatat gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga
    36781 cgcaacagaa acgccatcca gccaggttgc ggggtaaaga ttttctgctt acgcaactgc
    36841 cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca
    36901 tgtttcaacg gaccgataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg
    36961 gcaattttca ctggcgtttt aatgtcctgg cgggaataaa agccaggagc caacactttc
    37021 actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc
    37081 gcatcaaacg cggtaaattt accgtactgg aacagcgaaa cggtcaacgg acctgaaaga
    37141 atgcccaacg caaccgcact cggcagcgcc aacaggaaac aaagacgcag cccccagtcc
    37201 atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggcagc
    37261 aaaatggtgc caagcgccac gcccagcaca ccggacggaa actccattaa gcggtcggcg
    37321 taatacatcc acgacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt
    37381 aaggagatct ggctcacaga gacgccaagg attgccggtc ccatctgttt caccacacgc
    37441 attgctccgg catcgtggaa gttaatgcgt ggcaaaacca gcatgccgat cttcttcagg
    37501 tgcggtagct gatacaccag ctgcaggacg ccgccgaccg taacagccca cgccagcgcc
    37561 agcaccggtg ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta
    37621 agcagtgttg gagcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc
    37681 accagcgacg ccagggagat cagcaagata tagggaaagg taatctttag tagctggctg
    37741 gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat gatcacccac
    37801 ggtgcggcga gcatgccagc gaccgtcaca accgccagcg cgagtgtcag caggccagaa
    37861 acataagaga caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga
    37921 atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga
    37981 agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca
    38041 attgcgtcac gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc
    38101 agcgatttta ataaattcat cggtgttcta atccagaccc acgggcaaaa cgcctgcaag
    38161 gcaggcgctg aaagaggcga atagtctacc tggattatgg tgaattgcta ccgccagatg
    38221 ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag
    38281 cacggcctgt tcgccggcgg tttgcggaac tgtctgattt tgcacacatt caatgaagtg
    38341 ccgcgcacag ccgacaaatc cacgttgctc aagcgtgctc tgccaaccag gaatcggttt
    38401 attcactaca ccctgcccac gctcctcacg ccattcgcgc atttccgtaa tgtcgatgag
    38461 cgcaccgtca gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca
    38521 ggtggtgatc tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc
    38581 gtcgttagtc agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc
    38641 ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccggc
    38701 gctattgcta cgatgtttat ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa
    38761 ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg
    38821 cgccgccagt tccaccagcc gttcagcatc gcgcagattt tctgccagcg gtttatcgac
    38881 acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtgac tggcggtgct
    38941 ggaatgtaca aaaaccgcat cgcaactggc ggcaaggctg gataacgaat cggcataagg
    39001 aatgcgccag ctttcacaaa ttggcagggc tttcgcgcgc gtaggcgacc aggctccttg
    39061 taacgtccag tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc
    39121 taatcccact acgccgatac gtaatttttt cactttaatc tcccaggtgg gccagcaacg
    39181 aatcaagacg ctgtttcagt tctgccactt cgatttccag ggcttcgacg cgggcctgta
    39241 aatcaccgtc aaccgcgttc gacatatccg ttaccgccgg ctgatcctct acctcaccac
    39301 tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa
    39361 aaggaccatc ttcgcgattt gccagttgtt ccagcgtcga ctccacttcc gccatatcgc
    39421 tgaattcata cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca
    39481 ataacaacgt ggtgattaac gccacttctg cggcgctcag cttcagatcg ccaaattctg
    39541 aattacaaaa acgttgttca tatttggtga ctcgattacc aaagccactc accgtgcgca
    39601 gataatggcg ttttaccaga ttatccagtt gctcctgcac ctcggattca ctcagattca
    39661 tgaccggttc acggttcgtt ttctgattac aggccgttac tacaccattg actgagagcg
    39721 ggtattgctc cggcgtggtc acctgctttt ccagcaagca gccaatcacg cgggcttcca
    39781 gagcagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta
    39841 gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca
    39901 tagccttctt tttcaaaacc cagtcgtgcc agtaaatcac cgctgcgttt attgtgcggc
    39961 atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg
    40021 gtcagagctt caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga
    40081 tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc aataatctct
    40141 ttttcgtccg ggtcgaataa accaaagtag aaagctgaac cttgtttatg aaattcgtta
    40201 atcatcccca gcctggcctg ccagcctgat ggatagcagt ggctttcgtc gcgcactggc
    40261 tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgccagacg ccaggcatca
    40321 cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta
    40381 ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag
    40441 tattactata cctgcgcaac cagaccctgt gaaaagtccc aaatgccatt atctgcatta
    40501 ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atattgtccc
    40561 cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc
    40621 ccgggagggg aaatgtcccg cgtgtcgcag gcgaggagcc tgggtaaata tttcctgctc
    40681 atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc
    40741 ttcgttgatc aaatgggctg ggccgccgtc atggtcggta ttgctctcgg tctacgccaa
    40801 tttattcagc aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa
    40861 ccgatgattg ttaccggtat gctgatgcgc gccgccggat tcgccacaat gggtatcgcc
    40921 cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg gactcggcgg cacgttgttt
    40981 gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc cacaacagcg tggtcgtttt
    41041 ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca ttggcgcatt gttggggagc
    41101 tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggcagttct atttgtgctg
    41161 tgtgcggcgt tcaatgcgtg gttgttacca gcatggaaac tctccaccgt acgcacgccc
    41221 gttcgcgaag gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta tgttctgacg
    41281 ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat tatggtcaac
    41341 gacgtggctg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg
    41401 ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg
    41461 ttgatggctg ggctgttgat aatgtcatta agcatgatgc cggtgggcat ggtcagcggc
    41521 ctgcaacaac tttttaccct gatttgtctg ttttatatcg ggtcgatcat tgccgagcct
    41581 gcgcgtgaaa ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg
    41641 tttagccgtc tgggtctggc gattggtggc gctattggtt atatcggtgg cggctggctg
    41701 tttgacctgg gcaaatcggc gcaccaacca gagcttccgt ggatgatgct gggcattatt
    41761 ggcatcttca ctttccttgc gctgggctgg cagtttagcc agaaacgcgc cgcgcgtcgt
    41821 ttgctggaac gcgacgcctg atttgatact cccccctgac tcctccatac tgaaagtgaa
    41881 ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg cccttactgc
    41941 ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca tgttctgctc
    42001 aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc cattctgcaa
    42061 aaagatgaca gccgctatat gccagaaagc atggatatcg ttcactatgt cgataaactc
    42121 gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag
    42181 gtcaatggct acgccaacaa actgctgttg ccgcgttttg ccaaatcggc atttgatgag
    42241 ttttctactc ccgccgcgcg caaatatttc gtcgacaaga aagaggccag cgcgggtaat
    42301 tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga tgatttacgt
    42361 gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc ggaagatgat
    42421 attcagctat ttccactact gcgtaatctg acgctggtag ccggaatcaa ctggccaagc
    42481 cgcgttgctg attaccgcga taatatggcg aaacagacac aaatcaattt gttatcatca
    42541 atggcgattt aatcctgacc cgccgcggca gcctgtattc gcgggttgcc gccgttttca
    42601 gttttttctg ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc aggatattga
    42661 cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt
    42721 cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat
    42781 taaccagtcg cttgcgaaac ataataattt ctcaaaagat atcggtttac ccggcgtggc
    42841 tgacgcccat attgttctga caaacctgac cagccaaatt ggtcgcgaag agccgaataa
    42901 ggttacccta accggagacg ccaatctgga catgaactcc ctgttcggta gtcagaaagc
    42961 gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt
    43021 cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat
    43081 gcaaacgttg cttccctatt tgaaccaggc attacgcaat tactttaacc agcaacctgc
    43141 ttacgtcctg cgcgaagatg gcagccaggg cgaagcaatg gcgaaaaaac tggcgaaagg
    43201 cattgaagtg aagccaggcg aaattgtcat tccatttact gattaatcac gagggcgcat
    43261 tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa
    43321 aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg tattaaagat
    43381 ccgccgccca gacgactggc accttcacct ccgcgatggc gacatgttaa aaactgtcgt
    43441 gccgtatacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt
    43501 gaccaccgtt gaggctgccg tggcgtatcg ccaacgtatt cttgacgccg tacctgccgg
    43561 gcacgatttc accccattga tgacctgtta tttaacagat tcgctggatc ctaatgagct
    43621 ggagcgcgga tttaacgaag gcgtgttcac cgctgcaaaa ctttatccgg caaacgcaac
    43681 cactaactcc agccacggcg tgacgtcgat tgacgccatc atgccggtac ttgagcgcat
    43741 ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg acacatgcag atatcgacat
    43801 ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa ccgctgcgcc agcgcctgac
    43861 tgcactgaaa gtcgtttttg aacacatcac caccaaagac gcagccgact atgtccgtga
    43921 cggaaatgaa cggctggctg ctaccatcac tccgcaacat ctgatgttta accgcaacca
    43981 tatgctggtt ggtggcgtgc gtccgcacct gtattgtcta cccatcctca aacgcaatat
    44041 tcaccaacag gcactgcgtg aactggtcgc cagcggtttt aatcgagtat tcctcggtac
    44101 ggattctgcg ccacatgcac gtcatcgcaa agagagcacc tgcggctgcg cgggctgctt
    44161 caacgcccca accgcgctgg gcagttacgc taccgtcttt gaagagatga atgctctgca
    44221 gcactttgaa gcattctgtt ctgtaaatgg cccgcagttc tatgggttgc cggtcaacga
    44281 cacattcatc gaactggtac gtgaagagca tcaggttgct gaaagcatcg cactgacaga
    44341 tgacacgctg gtgccattcc tcgccgggga aacggtacgc tggtccgtta aacaataaaa
    44401 atcgtagctt cctgttgtca ttaggttatt ttacctgtat aaataaccag tatattcaac
    44461 agggggctat tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg
    44521 ctattgacgc cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg
    44581 aaggccacgt atcggtacgt tacgccgcag cgaataattt atcggttatt ggcgcgacaa
    44641 aagaagataa acagcgcatt agcgaaattc tccaggaaac gtgggaaagc gccgatgact
    44701 ggtttgtcag cgaataatat gcagtgattt ttgttt
//
LOCUS       DAZGBC010000017        43913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGBC010000017 DAZGBC010000000
VERSION     DAZGBC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.017"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            85..459
                     /gene="tomB"
                     /locus_tag="ACHK1J_001006"
     CDS             85..459
                     /gene="tomB"
                     /locus_tag="ACHK1J_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO7910319.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            485..703
                     /gene="hha"
                     /locus_tag="ACHK1J_001007"
     CDS             485..703
                     /gene="hha"
                     /locus_tag="ACHK1J_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO7910320.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            875..1426
                     /gene="maa"
                     /locus_tag="ACHK1J_001008"
     CDS             875..1426
                     /gene="maa"
                     /locus_tag="ACHK1J_001008"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO7910321.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1542..2012
                     /gene="ylaC"
                     /locus_tag="ACHK1J_001009"
     CDS             1542..2012
                     /gene="ylaC"
                     /locus_tag="ACHK1J_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO7910322.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2176..3726
                     /gene="pdeB"
                     /locus_tag="ACHK1J_001010"
     CDS             2176..3726
                     /gene="pdeB"
                     /locus_tag="ACHK1J_001010"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO7910323.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(3768..4121)
                     /gene="ybaA"
                     /locus_tag="ACHK1J_001011"
     CDS             complement(3768..4121)
                     /gene="ybaA"
                     /locus_tag="ACHK1J_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO7910324.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4241..4337)
                     /gene="ffs"
                     /locus_tag="ACHK1J_001012"
     ncRNA           complement(4241..4337)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK1J_001012"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4500..4811
                     /locus_tag="ACHK1J_001013"
     CDS             4500..4811
                     /locus_tag="ACHK1J_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO7910325.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(4842..5414)
                     /gene="ybaY"
                     /locus_tag="ACHK1J_001014"
     CDS             complement(4842..5414)
                     /gene="ybaY"
                     /locus_tag="ACHK1J_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO7910326.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5632..6492
                     /gene="tesB"
                     /locus_tag="ACHK1J_001015"
     CDS             5632..6492
                     /gene="tesB"
                     /locus_tag="ACHK1J_001015"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO7910327.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKKMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6541..7827)
                     /gene="amtB"
                     /locus_tag="ACHK1J_001016"
     CDS             complement(6541..7827)
                     /gene="amtB"
                     /locus_tag="ACHK1J_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO7910328.1"
                     /translation="MKIATIKTGLASLAMLPGLVIAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(7857..8195)
                     /gene="glnK"
                     /locus_tag="ACHK1J_001017"
     CDS             complement(7857..8195)
                     /gene="glnK"
                     /locus_tag="ACHK1J_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO7910329.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8376..10157)
                     /gene="mdlB"
                     /locus_tag="ACHK1J_001018"
     CDS             complement(8376..10157)
                     /gene="mdlB"
                     /locus_tag="ACHK1J_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7910330.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10150..11922)
                     /gene="mdlA"
                     /locus_tag="ACHK1J_001019"
     CDS             complement(10150..11922)
                     /gene="mdlA"
                     /locus_tag="ACHK1J_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7910331.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(11952..12410)
                     /gene="decR"
                     /locus_tag="ACHK1J_001020"
     CDS             complement(11952..12410)
                     /gene="decR"
                     /locus_tag="ACHK1J_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO7910332.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12564..13382)
                     /gene="cof"
                     /locus_tag="ACHK1J_001021"
     CDS             complement(12564..13382)
                     /gene="cof"
                     /locus_tag="ACHK1J_001021"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO7910333.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13482..15182
                     /gene="ybaE"
                     /locus_tag="ACHK1J_001022"
     CDS             13482..15182
                     /gene="ybaE"
                     /locus_tag="ACHK1J_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO7910334.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15247..15942
                     /gene="queC"
                     /locus_tag="ACHK1J_001023"
     CDS             15247..15942
                     /gene="queC"
                     /locus_tag="ACHK1J_001023"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO7910335.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLTDKP
                     TVMAAMKQKTGLK"
     gene            complement(15994..16392)
                     /gene="fadM"
                     /locus_tag="ACHK1J_001024"
     CDS             complement(15994..16392)
                     /gene="fadM"
                     /locus_tag="ACHK1J_001024"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO7910336.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16498..16869)
                     /gene="ybaV"
                     /locus_tag="ACHK1J_001025"
     CDS             complement(16498..16869)
                     /gene="ybaV"
                     /locus_tag="ACHK1J_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO7910337.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(17020..18891)
                     /gene="ppiD"
                     /locus_tag="ACHK1J_001026"
     CDS             complement(17020..18891)
                     /gene="ppiD"
                     /locus_tag="ACHK1J_001026"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7910338.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDKVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(19083..19373)
                     /gene="hupB"
                     /locus_tag="ACHK1J_001027"
     CDS             complement(19083..19373)
                     /gene="hupB"
                     /locus_tag="ACHK1J_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO7910339.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(19564..21918)
                     /gene="lon"
                     /locus_tag="ACHK1J_001028"
     CDS             complement(19564..21918)
                     /gene="lon"
                     /locus_tag="ACHK1J_001028"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO7910340.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(22106..23380)
                     /gene="clpX"
                     /locus_tag="ACHK1J_001029"
     CDS             complement(22106..23380)
                     /gene="clpX"
                     /locus_tag="ACHK1J_001029"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO7910341.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(23506..24129)
                     /gene="clpP"
                     /locus_tag="ACHK1J_001030"
     CDS             complement(23506..24129)
                     /gene="clpP"
                     /locus_tag="ACHK1J_001030"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO7910342.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGKSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(24375..25673)
                     /gene="tig"
                     /locus_tag="ACHK1J_001031"
     CDS             complement(24375..25673)
                     /gene="tig"
                     /locus_tag="ACHK1J_001031"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO7910343.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFSIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(26017..26334)
                     /gene="bolA"
                     /locus_tag="ACHK1J_001032"
     CDS             complement(26017..26334)
                     /gene="bolA"
                     /locus_tag="ACHK1J_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO7910344.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            26639..27217
                     /gene="yajG"
                     /locus_tag="ACHK1J_001033"
     CDS             26639..27217
                     /gene="yajG"
                     /locus_tag="ACHK1J_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7910345.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            27261..28736
                     /gene="ampG"
                     /locus_tag="ACHK1J_001034"
     CDS             27261..28736
                     /gene="ampG"
                     /locus_tag="ACHK1J_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO7910346.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            29195..30142
                     /gene="cyoA"
                     /locus_tag="ACHK1J_001035"
     CDS             29195..30142
                     /gene="cyoA"
                     /locus_tag="ACHK1J_001035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO7910347.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANDPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            30164..32155
                     /gene="cyoB"
                     /locus_tag="ACHK1J_001036"
     CDS             30164..32155
                     /gene="cyoB"
                     /locus_tag="ACHK1J_001036"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO7910348.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            32145..32759
                     /gene="cyoC"
                     /locus_tag="ACHK1J_001037"
     CDS             32145..32759
                     /gene="cyoC"
                     /locus_tag="ACHK1J_001037"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO7910349.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMAPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            32759..33088
                     /gene="cyoD"
                     /locus_tag="ACHK1J_001038"
     CDS             32759..33088
                     /gene="cyoD"
                     /locus_tag="ACHK1J_001038"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO7910350.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            33100..33990
                     /gene="cyoE"
                     /locus_tag="ACHK1J_001039"
     CDS             33100..33990
                     /gene="cyoE"
                     /locus_tag="ACHK1J_001039"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO7910351.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            34139..35503
                     /gene="yajR"
                     /locus_tag="ACHK1J_001040"
     CDS             34139..35503
                     /gene="yajR"
                     /locus_tag="ACHK1J_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7910352.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCIGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(35631..36122)
                     /gene="yajQ"
                     /locus_tag="ACHK1J_001041"
     CDS             complement(35631..36122)
                     /gene="yajQ"
                     /locus_tag="ACHK1J_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO7910353.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            36290..37201
                     /gene="panE"
                     /locus_tag="ACHK1J_001042"
     CDS             36290..37201
                     /gene="panE"
                     /locus_tag="ACHK1J_001042"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO7910354.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            37164..37754
                     /gene="yajL"
                     /locus_tag="ACHK1J_001043"
     CDS             37164..37754
                     /gene="yajL"
                     /locus_tag="ACHK1J_001043"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO7910355.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(37808..39256)
                     /gene="thiI"
                     /locus_tag="ACHK1J_001044"
     CDS             complement(37808..39256)
                     /gene="thiI"
                     /locus_tag="ACHK1J_001044"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO7910356.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGVDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            39462..39704
                     /gene="xseB"
                     /locus_tag="ACHK1J_001045"
     CDS             39462..39704
                     /gene="xseB"
                     /locus_tag="ACHK1J_001045"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO7910357.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            39704..40603
                     /gene="ispA"
                     /locus_tag="ACHK1J_001046"
     CDS             39704..40603
                     /gene="ispA"
                     /locus_tag="ACHK1J_001046"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO7910358.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            40628..42490
                     /gene="dxs"
                     /locus_tag="ACHK1J_001047"
     CDS             40628..42490
                     /gene="dxs"
                     /locus_tag="ACHK1J_001047"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO7910359.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRLMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            42545..43519
                     /gene="yajO"
                     /locus_tag="ACHK1J_001048"
     CDS             42545..43519
                     /gene="yajO"
                     /locus_tag="ACHK1J_001048"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO7910360.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEDLGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            43750..>43913
                     /locus_tag="ACHK1J_001049"
     CDS             43750..>43913
                     /locus_tag="ACHK1J_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910361.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKS"
BASE COUNT        10373 a        11642 c        10888 g        11010 t
ORIGIN      
        1 aaacgagagg gcaagtccag gtcagtaagt tttttccatc ccgaaaggtg tccgttagtt
       61 caaccgctaa gaaggggacg cgttatggat gaatactcac ccaaaagaca tgatatcgca
      121 cagcttaagt ttctctgtga aactctgtat catgactgcc ttgcaaacct tgaagaaagc
      181 aatcatggct gggttaacga cccaacctcg gcgatcaacc tccagttgaa tgaactgatt
      241 gagcatattg cgaccttcgc acttaattac aaaattaagt ataatgaaga caataagctc
      301 attgagcaga tcgacgaata tctggatgac acctttatgt tgttcagcag ttatggtatt
      361 aatatgcagg atcttcagaa atggcggaag tcaggtaatc gactattccg ttgttttgtc
      421 aatgcgacga aagagaatcc tgcgagttta tcttgttaga attattacaa ccatgggtag
      481 aagtatgtcc gaaaaacctt taacgaaaac cgattattta atgcgtttac gtcgttgcca
      541 gacaattgac acgctggagc gtgttatcga gaaaaataaa tacgaattat cagataatga
      601 actggcggta ttttactcag ccgcagatca ccgcctcgcc gaattgacca tgaataaact
      661 gtacgacaag atcccttcct cagtatggaa atttattcgc taataaataa ttcgctttcg
      721 gagctataac cggctgttta ttaagaattt tatacttttt cgccatgaag acatacccta
      781 tgtgatcttt atcacacaga tgtaatggga acgttctctt cactgacttt tcgtcttact
      841 gtgttgccgc attttcagca accggaggtc agtaatgagc acagaaaaag aaaagatgat
      901 tgctggtgag ttgtatcgct cggcagatga gacgttatct cgcgatcgcc tgcgcgctcg
      961 tcagcttatt caccgataca atcattccct ggcggaagag cacacattac gccagcaaat
     1021 tctcgctgat ttattcggtc aggtgacaga ggcttatatt gagccaacgt ttcgctgtga
     1081 ctatggctat aacatttttc tcggtaataa ttttttcgcc aacttcgatt gcgtgatgct
     1141 tgatgtctgc cctattcgca tcggtgataa ctgtatgttg gcaccaggcg ttcatatcta
     1201 cacggcaaca catcccatcg accctgtagc acgtaatagc ggtgctgaac tggggaaacc
     1261 cgtcaccatc ggtaataacg tctggattgg cggacgcgcg gtcattaacc ctggtgtgac
     1321 cattggtgat aacgtcgtgg tagcctcagg tgcagttgtc acaaaagatg tcccggacaa
     1381 cgttgtcgtg ggcggtaatc cagccagaat aattaaaaaa ttgtaatcgg tttttcgcaa
     1441 ctgttatgca aaattgtggt aaatctgtta ctttccctct actattccca cgttaaaata
     1501 gggtgttccc tggaaagttg cagataccac gaaggcaaac gatgaccgaa atacaacgcc
     1561 tgctgaccga aacgattgag tctctgaata cccgcgaaaa acgcgacaac aaaccccgct
     1621 ttagtatcag ttttatccgt aaacatccgg ggctgtttat cggtatgtac gttgcttttt
     1681 ttgccaccct ggcggtgatg ttgcagtccg aaacgctttc aggctctgtc tggctactgg
     1741 ttgtattatt tatcctgctt aatggtttct tctttttcga tgtctaccca cgctaccgct
     1801 atgaagatat cgacgtgctg gatttccgcg tttgctataa cggcgaatgg tacaacacgc
     1861 gctttgtacc tgccgcgctg gttgaagcca tcttgaactc tccgcgtgtc gcggatgttc
     1921 ataaggaaca actgcaaaaa atgatcgtcc gtaaaggtga attgtctttt tacgatattt
     1981 ttaccctcgc tcgcgccgaa tcaacatctt aagttaggat tacataccag gcgtaaagct
     2041 ctgcgcctgg tcaaatgaca acgatcgttt ccacccatca cttcatgaaa taccagctct
     2101 acctccttat ctcctgccag cctttttcca caatcagata tactttccct acactgtgtt
     2161 aataaggata tgctggtgag aacacgacat ctggtcggcc ttatttcggg agtactgatt
     2221 ctttcagtat tgctgcctgt cggcttaagc atctggctgg cccatcagca ggtagaaaca
     2281 tcgtttattg aagagctgga tacctattcc tcccgcgtcg ctattcgggc caataaggtg
     2341 gcgacacaag ggaaagatgc gctgcaagag ctggaaagat ggcaaggcgc tgactgtagc
     2401 gaagcccatc tcatggaaat gcgtcgggta tcttacagtt atcgctatat tcaggaagtg
     2461 gtttatatcg ataacaacgt tccccaatgt tcgtctctgg agcatgagag tccgcccgat
     2521 accttccccg agccaggtaa aatttcgaaa gatggttatc gtgtctggtt aacatcgcat
     2581 aacgatttag gcattatccg ttacatggtc gccatgggaa cggcacatta tgtcgtcatg
     2641 atcgaccccg cttcctttat tgatgtcatt ccctatagct catggcaaat tgatgccgcc
     2701 attattggca atgcccataa tgttgtcata accagcagcg atgaaattgc tcagggaatt
     2761 attaccaggc tacaaaaaac acccggtgag catatcgaaa ataatggaat catttacgat
     2821 atcctgccct tcccggagat gaatatttcg atcatcacct gggcttcaac gaaaatgttg
     2881 cagaaaggct ggcatcggca agtctttatt tggttaccgc tcgggttggt gattggcctg
     2941 ctggcagcga tgtttgtgct gcgtattttg cgccgcatcc agtcaccgca tcatcggctg
     3001 caggatgcta tcgaaaatcg tgatatttgc gtgcactatc agccgattgt ctccttagcc
     3061 aatggcaaaa ttgtcggtgc tgaggcactg gcgcgctggc cgcagacaga cggcagttgg
     3121 ttgtcaccag acagttttat tccgctggca cagcaaacgg gcctttctga gccattgacg
     3181 ctactgatta taagaagcgt ctttgaagat atgggcgact ggctgcgtca gcatccacag
     3241 cagcatattt cgatcaatct tgaatccacc gtgctcacct cggaaaaaat cccgcaattg
     3301 ctgcgtgaaa tgatcaatca ctatcaggtt aatcccagac agatcgcgct tgaactcact
     3361 gaacgcgagt ttgccgatcc gaaaaccagc gccccgataa tttctcgcta ccgggaggcg
     3421 ggccatgaaa tttatcttga tgattttggt acggggtatt caagtttaag ttatttacag
     3481 gatctggatg tcgacattct gaagatcgat aaatctttcg ttgatgcgct ggaatataaa
     3541 aatgtcacgc cgcatatcat cgaaatggca aaaacactga aactgaaaat ggtagcggag
     3601 ggaatcgaaa ccagtaaaca agaagagtgg ctacgccagc atggcgtgca ctacggtcag
     3661 ggctggctct acagcaaggc attaccgaaa gaagatttct tacgctgggc cgagcaacat
     3721 ttgtgaattt ttgggctgac ggcacgccgc acagcccgtc acgctaccta ttcgtcgatg
     3781 attgactcga atccgccata gatcattcgc ttgccgtcaa acggcatgga ctcgccgaac
     3841 tctttcatcc gtgggtccga catcatcttt tgattagctg cgtcgcggac ctcttttgaa
     3901 gggtattcaa tccagctaaa gacaacctct tcattctctt ccgctttcac cgccatacga
     3961 aaatcggtca ctttgccatc cggtacatcg ctggcccagc attcgacaat acgaagcgcg
     4021 ccaaactctt taaacaatgg cgcggcctta gcggccattt cccgataggc atccttttta
     4081 tcggcaggaa cggcaaccac aaaaccatca acatacttca tcagacatac ctccgaagcg
     4141 tactgcgcag ccagacaatt cttgcgacgt cagttgtggt taaagtttag tcacgctggg
     4201 attttttgac gaaacggtgg gaagcagaaa tgggtggggg ccctgccagc tacatcccgg
     4261 cacacgcgtc atctgccttg gctgcttcct tccggacctg acctggtaaa cagagtagcg
     4321 ttgcgggaga accaacagag cccccattga gagcgttgag aaccaacgcg caggcgcatt
     4381 atcactgctg ccccggctaa ttgcaaccca ttgcgtgcca gatgctggtt tcttgcgcaa
     4441 tgcgacttca ctcgggcgtt tttccagatt atgctgaaaa attacctcag gaagaaaaga
     4501 tggaaaaaga agattcattt ccccaacgcg tctggcaaat cgtcgccgct attcccgaag
     4561 gctatgtaac cacttacggt gatgtggcga aactggcggg atcgccccgc gccgcgcgcc
     4621 aggtgggcgg tgtgttaaag cgtctccctg aaggcagcac cttaccctgg caccgggtgg
     4681 ttaatcgcca cggcacaatt tcgctaaccg gaccggattt acagcgtcag cgacaggcat
     4741 tactggcaga aggtgtgatg gtatcgggaa gcgggcaaat cgacttgcag cgttatcgct
     4801 ggaactacta accctctccg ggcggagagg gttaacgtcg cttaatattg cgtaggagct
     4861 ggaactgccg aagacggatt cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg
     4921 ccaccgctgg cctgaaccgg cacggcagtt tgctgtaccg gcaccaatgt caggtcggct
     4981 ttagttccgc cctggttgat caccggctga acggtatcgg tgataaatac cagtttgtca
     5041 ttcacggtaa tcgccgcact caacagaata cgcgcgttcg gctgaacatc tgccgggtta
     5101 aatggcagaa caaagctgaa tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc
     5161 agcacttttg acggtgcatc ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca
     5221 tcaggcggca gtgcgacttt ctgacggatc cagacggtac cggagacatt cggttgctgg
     5281 atagcaggtt gttgtgttgc tgaaatagac gtatttgcag ccggggctgg cgtctgaata
     5341 tccgcgcttt tatctgcgca agccgccaac gcaatcgcaa ccgctaaacc actggccatg
     5401 tgcacgagtt tcattcattt ctccttatta tcaatgcacc agcgggctaa ctttcctcgc
     5461 cggaagagtg gttaacaaaa tagtaacgtc aacaagtgtg gcacacatca cgcatttctg
     5521 cctgtaatta gcccgtaatt cagaccattg cacccatcgg accagtagca aatttgcgtt
     5581 atactcaact cactttggct tgctgcggca gctttgttac tggagagtta tatgagtcag
     5641 gcgctaaaaa atttactgac attgttaaat ctggaaaaaa ttgaggaagg actctttcgc
     5701 ggccagagtg aagatttagg tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc
     5761 ttgtatgctg caaaagagac cgtccctgaa gagcggctgg tacattcgtt tcacagctac
     5821 tttcttcgcc ctggcgatag taagaagccg attatttatg atgtcgaaac gctgcgtgac
     5881 ggtaacagct tcagcgcccg ccgggttgct gctattcaaa acggcaaacc gattttttat
     5941 atgacagcct ctttccaggc accagaagcg ggtttcgaac atcaaaaaaa aatgccgtcc
     6001 gcgccagcgc ctgatggcct gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg
     6061 ctgccgccag tgctgaaaga taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg
     6121 gagtttcata acccactgaa aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc
     6181 gcaaatggta gcgtgccgga tgacctgcgc gttcatcagt atctgctcgg ttacgcttct
     6241 gatcttaact tcctgccggt agctctacag ccgcacggca tcggttttct cgaaccgggg
     6301 attcagattg ccaccattga ccattccatg tggttccatc gcccgtttaa tttgaatgaa
     6361 tggctgctgt atagcgtgga gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt
     6421 gagttttata cccaagacgg cgtgctggtt gcctcgaccg ttcaggaagg ggtgatgcgt
     6481 aatcacaatt aaaaaaatag ccggaggtga aaaccgtccg gctgtttttt gcagtgcttg
     6541 ttacgcgtta taggcattct cgccgtgact gttgacgtcc agcccttctc gctcttgttc
     6601 ttccggcaca cgcaggccaa ccgtcagatc cgccagtttg tagccaataa atgccaccac
     6661 accggaccag acgatcgtaa tggcaatgct ttccaactgt accagcaact gatggcccat
     6721 cgtcacacct tcagcgaagc ccacgccgcc cagcgaactg gcggcaaaaa tcccggtcat
     6781 gatacagccg acaatgccac aaacgccgtg cacaccgaag acatcgcagg gatcatccac
     6841 ccgcagcaag cgtttgagca tggtaacgcc ccacaagccc gccagaccag ctaccacgcc
     6901 gataatcaac gcgccgccaa ccccaatgta gccacaggct ggcgtcacgc cgaccagacc
     6961 ggcaatcgcg ccagaacacg cccccagcag tgaaggctta ccacgcagtg cccattcacc
     7021 gaagatccag ccaagaattg cagccgccgt tgcgaccaca gtattcacaa aagccagtgc
     7081 cgcgatttca ttcgccgtgc ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag
     7141 aatggcagtc ccggtgaaga ccatcggcag gttgtgtggt ttaaacgcct ctttaccgaa
     7201 gcccacgcgt tttcctatca gatacgcgcc caccagaccg gcgattgcgg cgttaatgtg
     7261 caccaccgtg ccacccgcga aatccagcgc accgtgagaa gccagcaaac caccgcccca
     7321 gaccatatgc gcaattggaa tgtaagagag cgtcagccat accaccacga aaatcaacac
     7381 agctgagaag cggattcgtt ccgccagcgc cccaactatc aagcctacgg taatgcaggc
     7441 aaacgatccc tgaaacgcta cgtggatata ctgataaatg ctgcccatca ccgccgtcag
     7501 ttcgatgttt ttcagcatca gccagttaat gttgccgaag aagttgttgc cctcaccaaa
     7561 cgccagcgag taaccgtaaa ccacccagag aatacagacc agtgcaaacg tcaccgtcac
     7621 ctgcgtcagc atcgacagca cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat
     7681 ccccggaata gtcataaaca gcaccagcgc agtacaaatc atcataaacg cattgtcggc
     7741 tttatcggcc accgcaggtg cagctattac cagtcccggc agcatcgcca gtgaagcaag
     7801 cccagttttt atcgtcgcta tcttcatttt tcgttcctgt tgctgtgtgc cagagattac
     7861 agcgccgctt cgtcggcttc gccggtacga atacgaatga cgcgttgcaa ttcagcgacg
     7921 aagattttgc cgtcgccaat ttttccggtg taagccgcct tactgacgat atcgatcact
     7981 tcatcgagtt ggtcatcagc aatcgccaca tcaattttta cttttggcag gaagttgacg
     8041 ctgtattccg ccccccggta cagctcggca tgccctttct gacgcccgaa acctttcact
     8101 tcggtgacgg tcaggccctg aataccaatg gaagataacg cttcacgaac gtcttccagc
     8161 ttgaatggtt tgattatcac ggtcaccagc ttcatagatc ccctccggtc agaattcggt
     8221 aatggttctg ctacacgaag aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa
     8281 agagagcagg aagttaagaa gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt
     8341 tacatttttg caccaacgat gaaatattgc gctattcagg cgctcaatga ttcttcttca
     8401 cgcacgctgg ctgccagctc ttcgcccgca agttgcagtt gatacatctg ccagtagcgt
     8461 ccctgggccg ccagcagttg ctggtgagtg ccctgctcca cggcttgccc acgatgaagc
     8521 accagaatag tgtcggcatc aacaatggtc gataagcggt gagcaatcac caccagcgtg
     8581 gtatgttcac gcaccgccgc cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg
     8641 ctggcggttg cctcatcaag gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt
     8701 gccagcagtt gcttttgccc gactgagaga ttatttccct gctcgcccag cggcgtgtaa
     8761 ataccgtcgc tcatgctacg cgccagctcc gccagttgca cggtttccag cgcctgccag
     8821 acgcgttctt cggagatatc acgccccagc gtcacgttgg cgaggaaggt atccgccagc
     8881 accaccggat cttgctgcac cattgccacg ccctggcgca gcgcgctgtg acttagcgaa
     8941 cttaatggac gaccatcaag gcgaatctca ccttccgtta gcgggtaata gcccatcaat
     9001 aaactggcga gggtactttt accactgccg gtatgcccga ccagcgccac aaagttacgc
     9061 gaaggcacag agagattaat gttctttagc accagattgt catcgcgata agcaaatgac
     9121 acgttatcga cttcgatggt gccgctctgt aacgggcgat catcattgcc atattgctgg
     9181 cgcggtccgt ccatcagctc aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc
     9241 gcctgctgcg tggtcagttc gattaagggt tcgttaagtc gcccaagata gctgataaac
     9301 gcatacaaca cgcccacttc aatggtgccg ctggcggaga agccaaacag catcaacaag
     9361 ccacagagaa tgagcgatga aaacagactc agcagcggac gcaggagaaa accgtcgagg
     9421 cgcagggttt gcatcctcgc catatagtgt gaacgactgg cctcacccat gcgttcgcca
     9481 aatcgcgcct gctggcggaa ctgctggata acgctcatgc cattgatgat ttcgttaaag
     9541 ccgtcgttga tatccgccag ataggcgcgc acacgacgga caatcggcgt gctgtaacgc
     9601 tggtatatca ccattaccac cagcaccacc gggaaaatca ttatcgccac cagcgccatt
     9661 cgccagtcga ggctgaacat cgccaccagc atcgcgccca ccagtgcggc actgcgcagg
     9721 acagttgcca ctacggtaac gtagagatcg cggatcactt cagtgtcatt agtgacgcgg
     9781 gaaatcacct gcccgacggg ttgggtatca aactcgctta atggctggcg taacgcagca
     9841 tccatcacgt cggtacgcaa ctgttgcact acgcctactg ccgcccgatt aaacagcagc
     9901 gactgcgcgt aatgtagccc ggcggcaaac agttgtagcc caacatacgc cgcagccagc
     9961 cctgcaacca ctttcaacgg caggttattt ttcgctacca tattgtcgat aaaatagctg
    10021 ataagcagcg gcccactgac ttctgccgct gccgcaaccc acatcatcag gaccgcaatc
    10081 cccagcggtt tacgccacgg cgaaccgtac gctaacagac gcttgagagt cggccacagt
    10141 tggctaaaac tacgcatcga cggcctcctc gcgaatttcc ggagcgtcgt cgagcgccgc
    10201 ctccagttgt tgatagcgat acatatcgcg ataccagcca ctttgttgcg ccagctcatc
    10261 atgattgcca cgctgggcga tatgtccgtg ctgcatcaca ataatttcac tggcttccgt
    10321 cagtgcagaa aggcgatggg cactgatgat taccgttctt ccctgccccc actgacgcag
    10381 gttatgcagg atctggtgct ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat
    10441 gaggatttcc gcgttgacta ataacgcacg agcaatggag atacgctgtt tttgcccgcc
    10501 ggaaagcatc acaccgcgct cgcccacctc tgtatcgtaa ccttgcggta gacgcaaaat
    10561 atcgtcatgt acgctggcta accgcgcgac atgctcaatc tcttgctggg tggcattcgg
    10621 gcaacccagc gcgatgttat tcgccacggt gtcagaaaaa aggaatggcg tctggctaac
    10681 taccgccagg cggctacgcc agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa
    10741 gcgaatatcc ccctcgctga cgtcgaaatg acgctgaatg agcgacaaca gggtactttt
    10801 gccggaacca gtcggcccgc agatacccag catctgaccg ggtttcaggg cgaaattgac
    10861 gttttccagc gcaggatggt cagtctgcgg atacgtgaac tggtgaatat ttacatccag
    10921 ttcgccacgc ccttccggca cgggttcgct accatcgttc accaccggcg cttccgccag
    10981 catcgcgcga atacggctgt acgcagcact accacgttcc acaatgttaa acatccatgc
    11041 cagcgccagc attggccaaa tcatcagacc taaatacatc ataaaactgg tgagctggcc
    11101 cagcgttaaa ctgccctgca ccaccatcca actaccgccg ccaatcgcca gcaagttcgc
    11161 cataccaatc gcgatataga tggtcgggtc gaaacgagca tcaatacgcg ccacccgcat
    11221 gttttttttg ccggtatctt cggcatccgc ggcaaataac gccgactggc gatcttccag
    11281 accaaaggct ttgatcatgc ggatactggt gaggctttcc tgggtgcggt cattaagact
    11341 ggaaaacgcc gcctgtgcca gcttaaagcg ttcatgcaaa gcatcgccgt tgcgcttaat
    11401 catgatcgcc atcactggca tcggtaacag ggcaaataag gtcaactgcc agctaatttg
    11461 cgtagacatc ataatcaaca cagcgcagcc cattaccagt gaatccacca gcgtcagcac
    11521 cccttctccg gcggcaaaca cgacgcgatc gacgtcatta gtcgcacgag ccatgaggtc
    11581 acccgtgcga tgacgcaggt aaaactcagg atgctgccgg cttagctgac ggtaataatc
    11641 ttcacgcagt tcaacagcca gttgataaga cgcaccaaac agcaatacgc gccagacgta
    11701 acgcaggaga taaaccacaa cggcaatcag caccatggtg gcgatccaca tcaggatctg
    11761 cccggtagta aagtgttgtt ctgtcacgcc atcgacaaca ataccaacca cttttggtgg
    11821 aaccagttgc agcatcgcga taatgacaag caaggcgaca gccccgagat agcgacgcca
    11881 ttcccgacgg aaataccagc ttaattgagc aaataatcgc acgcgttgtg tcctgacctg
    11941 attctggata tttattcgat gggtaaagaa gtggtgtatt taatctgttc catcgcgaag
    12001 ctggaagtga cgtccgacag ccccggcacg ctgtttacca gacgcttata aaactcgtcg
    12061 tagcgtttca tgtcggcaac ctggacgcgc atcagataat cgtattcacc agccatgcgc
    12121 cagaacccca gcacttctgg catttcggta accaccgtga caaagcggca ataccattcg
    12181 ctgctgtgat gttgcgtttt tatcagcaca aaggcggtca ggccgaggcc tattttttcc
    12241 ggatccagca gggcgacttt gccgataagg ataccgtcgt cctccagccg tttcaggcgc
    12301 ttccagcaag gggtggttgt cagattaacg gcttcagcca gtgcctgcaa agagagggtg
    12361 caatcctgct gcagtaaggc cagcagctta cggtcaattt tatctaacat agcccttcca
    12421 cagagaattt ttttctcttt taattctatt ttaaaggtga aatcgcgaca atttattctg
    12481 tgcggtttaa acaccacggc acaaaatgac aaattcaacg caaaaaaaag ccgggcaatt
    12541 gcccggtgct ggaaggaatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    12601 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    12661 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaatccgct accgacgctg
    12721 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    12781 cctaaatgtt gggtcagtac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
    12841 tcaaggcaat ccgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    12901 tggatctgca aacgtgtaag atcgtcgtga tcgccacaga agcagatctt ggtgacgctg
    12961 ccgagtggca tttttttgac atcgattatt tgataacgaa aaccgctata gacaaatgcc
    13021 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
    13081 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    13141 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    13201 tacgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    13261 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    13321 cctaaatgat gatcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    13381 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    13441 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgactg ctcaaccgtc
    13501 ttaaccagta tcaacgtctg tggcaacctt ccgccggaaa gccgcaaacc gtcaccgtca
    13561 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    13621 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    13681 tacgctttct ggtcacgcca gaatcgctac gcaatgcgat gatggaaaag gcactggaaa
    13741 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag ctgcgcactc
    13801 tgttacagcc gtttatgggc ggacaatggc aaaacgatac gcccacgttg cgtattccct
    13861 actatcgccc gctcgaaccg ctacaaccgg gctttttgcc cggccgtgcc gagcagcatc
    13921 tcgccggaca gatattttcc ggcctgaccc gcttcgataa taatacccag cgcccgattg
    13981 gcgatttagc gcatcactgg gaaacctctg ctgacgggtt acgctgggac ttttatcttc
    14041 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    14101 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcgtattg
    14161 aggtcaccca tccgcagtgt ctgaccttct ttttacatcg ccctgattac tggcttgcgc
    14221 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcacgg
    14281 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggaaagc catgattatt
    14341 accatttacg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    14401 aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    14461 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    14521 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtaatctcc attattcatc
    14581 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    14641 tactgccagg ctggactatt ccgcattggc aagtaccgga tgaagtcaaa ctaccgaaaa
    14701 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
    14761 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
    14821 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    14881 caccggaata tactctggag caatggctac gttgcgatcc actgtggcca catgttttcg
    14941 acgctccagc atatgcccat ttgcaatcga cactggacgc ggtgcaagta atgcctgatg
    15001 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    15061 tgacgccgct attcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    15121 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    15181 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    15241 ttatctatga aacgtgctgt cgttgtgttc agtggaggtc aggattccac cacctgtctg
    15301 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcgc
    15361 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggggc acgcgcgcat
    15421 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac gcgtgacagc
    15481 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtcccaggg
    15541 cgtaatattt tgttcctgac actggcggca atatatgcgt atcaggtaaa agcagaagcc
    15601 gtaattactg gcgtctgcga aacggatttc tccggctacc cggattgccg cgatgagttt
    15661 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    15721 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    15781 gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga cggttgcggt
    15841 cattgtgcgg cgtgtaattt acgcgccaac ggtttgaacc attatctgac cgataaaccg
    15901 acggtgatgg cagcgatgaa gcagaaaaca gggttgaagt aattattcca agtattgccg
    15961 gatgcggctt gagcatccgg cacaacaaaa cgtttactta accatctgct ccagcttttc
    16021 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    16081 aataagcgca tccgctacta cctgcccttc cggctccagt gtaatgacct ggcttaagat
    16141 gccgctttta ccgtttaatt gctgtaactg gctcgtaatg gttaacaagt cgcttaatac
    16201 cgccgggcga cgatagttaa tattgatatt caccaccacg aaagcgatgt tatgggccgt
    16261 catccagtga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    16321 aaggtagcgg gcgttgttta cgtgctggta aacgtcgaga tgatatccac gaactttgat
    16381 ttgtgtttgc atagcgcaat aaccttacgt tgtttttata aaaactgtta taagtacaga
    16441 ggtcataact ggtatgacct cttcagtctg gcaaaaaatt gccactatgc aaattaatta
    16501 cagggttaat accgccagat tacgttccac cagcgaattg cccatccccg gcacctgctt
    16561 tagatcctcc accgttttaa acggaccgta ctcttcgcga taactgacaa tcgcctgcgc
    16621 tttcttcagg ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg cattattaat
    16681 gctgacccgg gtgccttctt cgtcactggc tttcgccggt actgctgctt tactttgtgc
    16741 tgcaggagct tccgctttgg tttctaccgc cgtcggtttc gccacagaag ctgccgccag
    16801 cgcgctatga gacattccgg cacaggccag ggacagggta atgagcagtg ctttaattcc
    16861 gtgtttcata ctgttttctc cttgtttgct aacagtggaa tcaccttaac gggagtgaaa
    16921 acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac cttttcttgt
    16981 tacagctgca aagcattttt ttgcgaggcg gcttcaggat tattgctgtt ccagcgcatc
    17041 gccaattttg attttcgcct ctttacgcag gttactcatc agagcttcaa agacgatttg
    17101 tgcgttgttc tgggtgatac cctgcaccat cgcttttttc tgatcttccg gcattgaacc
    17161 ttgtttcact tcatccagcg ccagcagaac cacattacct tgcatatcgg tcgccatacc
    17221 gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc taatcgggtc
    17281 acgaccggaa cggcttaagg ttttcggctc tccaaatttc agaccggcag cctgcatagc
    17341 ttccgcacct ttgccggctt tcaaatcaac cagcagtttc tcagcatcca ctttcgcctg
    17401 ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt tcctgaacat ctgccaacgg
    17461 tttcaccgct tccggtttgt gctcgctgat gcgcagcacg aatgcgcggt cgccgtctac
    17521 ggtgatgatg tcagagttga tgcccggcgc gccgttttca cctaccagac cgccgttaaa
    17581 gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc aggttatctt tgctgaacca
    17641 acccgtctga gtggctttaa cgccggcagc ttgctctgca ccggccagag actcggtgtc
    17701 gttgcttgcc gcatcgctca ctttctgctg cagcgcgtag tacgcatcga gggctttttc
    17761 gtgtttcact ttcgccgcaa tgtcgtcacg tactttgtct aacgatttca ctttcgctgg
    17821 ctgaatgtcg tccagacgta caatcaggaa accgaccgaa gatttgatga caccagacag
    17881 ttggcctttt tctttcagac cagcattttt cagttcatcc gggatagtgg catcttctaa
    17941 ccaacccata tcgccgccgt tacgagcaga gataatatcg gcagattttt ctttggctaa
    18001 tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc gctttcgctt catcttcagt
    18061 tttggtctgg atgatgctgt agcgggtacg ctgcggctgg gtgaattgat cctgatgctg
    18121 gtcgtagtag ctctggatat ccgcatcgct aaccggttgc tgcatcgttg cggcatccag
    18181 cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt tttgttcgta
    18241 gtagctggca atctcctgtt cggtcacagg ctgcttcgcc gccagcgcgt taacatcgat
    18301 agtcgcttca cgtaccacgc gttgttgagc gactagtgcc gccagctcgt cggtttcacc
    18361 tttcagcata aaatcggtac cggcaacgcc gttaatcagc tgttgggtag tgagctggtt
    18421 acgcagcgcc tgggcgtact gatcggcggt catccccatc tggttgagga taccgttata
    18481 gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa tcgcctgttt
    18541 aacctgctca tcgctgatac ccagtttcag ctcacgtgcg tactgatcca gcagcgcctc
    18601 gtcgatcaga cgattcagca cctgttgacg cagggttttc atatagcctt cgttcgctgc
    18661 cagctcggag tattgatcgc ccagctgttg ctgcatgcga ttacgctcgc tgttgaaggc
    18721 attctcgaac tgcccacggc tgatttcctg gtcattcact tttgcggcgt agttattgcc
    18781 tccgccaatc aggtaaccac tcacgccggt caatatgaac gacacgataa tgataccgaa
    18841 aataatcttg agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca tggtgtaaca
    18901 acactccgct gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc aaaggcctat
    18961 tgtgacaaga aacgggggca attgtcagcc cacaagacac agaaagtcac cgaatacaaa
    19021 taaaaaaggc acatcagtag atgcgccctt gaacttcgtc acatccccac tggggacaac
    19081 gcttagttta ccgcgtcttt cagtgcttta cctgcacgga agctcggtac tttagcggca
    19141 gcgatggtga tctctttacc ggtctgcggg ttgcggccag tacgggcagc acgctcttta
    19201 acggcaaaag taccaaaacc taccagtgct acatcatccc cttctttcag agattcagtt
    19261 acggaagcaa taatagcatc taacgcacgg ccagccgcag ctttagagat atcagcccct
    19321 gcagcaatct tgtcgatcaa ttgagattta ttcactcttc tcttcctctt tataatttat
    19381 atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc agccatcccc
    19441 ttacaacacc ccttaataag ggcaagcccg atccgccatc taacttagcg agacaaaaaa
    19501 aggctggcaa gcccgaatta gcctgccagc cctgttttta ttagtgcatt ttgcgcgagg
    19561 tcactatttt gcagtcacaa cctgcatgcc agacggttca ttttgcagcg ccagagtcag
    19621 aacttcctca atgcgcttca caggatgaat gtccagatcg gcaattacgt tgtcaggaat
    19681 ctcttccaga tcgcgtttat tttcgaacgg aattaacact gttttaatcc caccgcgatg
    19741 cgctgccagt agtttttctt tcaaaccacc gatcggcagt acctgaccac gcagagtgat
    19801 ctcaccggtc attgccacat cggcacgaac cgggttaccg gtcaggcaag aaaccagcgc
    19861 ggtgcacata gcaataccag cactcggacc atctttcggc gtcgcacctt ccggtacgtg
    19921 gacgtggatg tcgcgttttt cgtaaaaatc agggttgatc cccagttttt ccgcacgcgc
    19981 acgaaccacc gttaacgccg cctgaatgga ctcctgcatc acttcgccga gcgaaccggt
    20041 ataggtcagt ttgcctttcc ccggaacgca cgcggtttca atggtcagca agtcaccgcc
    20101 cacttccgtc cacgccagac cggttacctg gccgacacgg ttttcgttat cagcgcgtcc
    20161 atagtcgaaa cgctgaacac cgagatagtc atgcaagtta tcgccgttaa tttcgatatg
    20221 ttttaatgac ttatcgagca gtaactgctt aaccgcttta cggcacagtt tggagatttc
    20281 acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa cgaataatgc cgataatggc
    20341 gctatcgtcg acggtcagct cacctttttt cagtgcatta cgttcaatct gcttcggcag
    20401 caggtgacgt ttggcgatgt tcagtttttc atcttcggta taaccggaga ggcgaatcac
    20461 ttccatacga tccagcagcg gtgccggaat gttcatggag ttcgacgtcg cgacaaacat
    20521 cacgtcgctg agatcgtaat ccacttccag gtagtggtcg ctgaacgcta cgttctgctc
    20581 tggatccagc acttcaagca gtgcagaggc cggatcgcct cgcatgtcag aagacatttt
    20641 gtcgatctca tcgagcagga acagcgggtt tttcacgccc actttcgcca ttttctggat
    20701 cagtttaccc ggcatagaac cgatgtaagt acggcggtga ccacggattt ccgcttcatc
    20761 acgcacgccg cccagcgcca tacggacata tttacgcccg gtggctttgg cgatggactg
    20821 acccagggag gttttaccta ctcccggcgg ccctaccagg cagaggatcg gtcccttgat
    20881 tttgttgaca cggctttgaa ccgcaagata ctcaaggatt cgatctttca cgcgctccag
    20941 accataatgg tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt tgaccttgct
    21001 gcgcgcattc cacggtacct gtaccatcca gtcgatataa ccacgcacta cggtcgcttc
    21061 tgccgacatc ggagacatca ttttcagctt ctgcaactct gcttccgctt tctcttttgc
    21121 ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg gcttcgtttt cgtccggcgc
    21181 gtcgtccatt tcaccgagtt ctttctgaat agctttcatt tgctcgttca gatagtactc
    21241 acgctgggat ttctccatct gctttttaac gcggttgcga atgcgtttct caacctgcag
    21301 cagatcgatt tccgattcca tcattgccat cagatattcc agacgttcgt taacgtcgga
    21361 catctccaga acggactgtt tgtcagccag tttcagcggc atatgtgcag caatggtatc
    21421 cgccagacgc gccggatcgt cgatgctatt cagcgacgtc agcacttctg gtgggatttt
    21481 tttgttcagc ttgatgtagc cttcgaactg gctgattgca gtacgcacca gcacttcctg
    21541 ttctcgctca tcaatggtcg gcgactccag atactccgcc ttcgcagaaa agtgttcgcc
    21601 attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca ctttgacggt
    21661 gccgtcaggc agtttcagca tctgcaatat agaggccacg gtcccgacgg tgaaaagatc
    21721 gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg accagcataa tttttttatc
    21781 atggtccatc gccgcttcca gacaacggat agatttttcc cgcccgacaa ataaggggat
    21841 gaccatgtgc ggataaacca ccacatcgcg cagcggcaat acggggattt caatgcgttc
    21901 agaacgctca ggattcatag agctctctct tagtttaatt tccgccaggt aatcagatga
    21961 cacgactgtg cttcacgcca tctattaaca tgtacgtcag tatatgggga tgtttccccc
    22021 acattcaacg ccgagaatag aggaaaaatt aaaggggaga taaaatcccc cctttttggt
    22081 taactaattg tatgggaatg gttaattatt caccagatgc ctgttgcgct tccggcttgc
    22141 cataaatcag caacggtttg ctttgaccat caattaccga ctcgtcgata accacttttt
    22201 cgacatcttc catggacggc agatcgtaca tggtatcgag cagtgcggct tctacgatgg
    22261 aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt cttagcgata gcatccagcg
    22321 cctcgtcacg gaattccaga tccacgcctt ccagattaaa cagcgcctga tactgcttgg
    22381 tcagggcgtt tttcggctct ttgaggatct gaatcagagc ttcttcgctc agttcattca
    22441 acgttgcgac aaccggcaga cgaccaataa actcagggat aagaccaaac ttgatcagat
    22501 cttccggttc aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac ttcgctttta
    22561 ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg atgggaaatc actttatcca
    22621 gaccggcaaa cgcaccgcca caaataaaca ggatcttaga ggtatcaacc tgcaagaatt
    22681 cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc agctaccgta ccttcgatca
    22741 gtttcaacag tgcctgctgt acgccttcac cggaaacgtc acgggtaata gacgggttgt
    22801 ctgacttacg agaaatcttg tcgatttcat cgatgtagac aatcccgcgc tgcgctttct
    22861 gtacgtcgta atcgcacttt tgcaacagct tctgaatgat gttttcaacg tcttcgccca
    22921 cataaccggc ttcggtcagc gtggttgcgt cggccatggt gaacggaacg tccagcaggc
    22981 gcgccagcgt ttcagccagc agcgttttac cggaaccggt cggaccgatc agcagaatgt
    23041 tacttttgcc caactcgacg ccattgctgg tatcgccgtt gcgcagacgt ttgtagtggt
    23101 tgtataccgc gaccgccagc acttttttcg cctgttcctg gccgataacg taatcgtcca
    23161 ggtggttacg aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc ggtgcaactt
    23221 ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg cagatataca
    23281 cggatggacc ggcaatcagc ttgcgcactt catgctggct tttgccgcaa aaagagcaat
    23341 acagcaattt gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac ctcttctttg
    23401 ttctttgtgc cgcacacgac gacgcaaatg ccattctcag gcgcaagccg cttacagcgt
    23461 tgtgccgccc tggataagta tagcggcaca gttgcgcctc tggcatcaat tacgatgggt
    23521 cagaatcgaa tcgaccagac cgtattccac cgcttcaggg gcggaaagga agcgatcgcg
    23581 ctcggtatca cgttcaatct gttctaatga tttacccgta tgaagcgcca taagttcatt
    23641 catacgccct ttaactttca gaatttcacg ggcatgaatt tcgatatcgg tcgcctggcc
    23701 ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa ttcggcaggc aaaaacgttt
    23761 accttttgcc cctgcggtca gcaagaaagc gcccatcgag gccgcctggc ccatacagat
    23821 ggtgctgaca tcaggcttga taaactgcat ggtgtcatag atagacatcc cggcagtgat
    23881 caccccgcct ggggagttaa tgtacagata gatatctttt tctgggtttt ctgcttccag
    23941 gaacagcatc tgcgccacaa tcaggttagc catgtggtct tcaacctggc cagtcagaaa
    24001 aatgacgcgt tccttaagta gacgagaata gatatcaaaa gagcgctcac cacgtgaggt
    24061 ctgttcaatg accatcggca ccagcgccat atggggtgca aagttatctc gttcgccgct
    24121 gtatgacatt tccgtctcct ggataaaatt gaaaaaacct gctgtaccga ttgtaacctt
    24181 atggacggat tatcagctag tccctgtgtt atgggtacgg catcaccata tttcaagcat
    24241 aacaatcttt tgttgttacg ctaacaccga aagaggcttt tcgcacggtt ccatgcaaaa
    24301 ttcatgacaa aaaaaagccc accacctggc ggtgacgggc ctttgtgcga atttagcgcg
    24361 ttatgctgcg taaattacgc ctgctggttc atcagctcgt tgaaagtggt ttctttttca
    24421 gtcactttcg ctttcgccag tacagcttca acagcctgtt cttccagagc aacattgcgc
    24481 atgttgtcca tcagttcttt gtttttgctg tagaactcga taacttcttt cggatcttcg
    24541 tacgcagaag ccatctcttc gatcaggcct ttcacgcgct cttcgtcagc tttcagctcg
    24601 ttggtgcgga taacttcgcc cagcagcagg ccaacaacta cgcggcgttt agcctgttct
    24661 tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt tgccaccgaa acgctgtgca
    24721 gcctggcgac gcagaacgtc gatttcgctg tcaatcagcg cagccggtac gtcgatgtcg
    24781 ttagctttta ccagaccttc gatcgcctga gacttaacgc ggttacggat ggcgctcttc
    24841 agctcgcgct ccatgttttt acgcacttca gcgcgcagac cttctacgga accatcttca
    24901 acgccgaaac gtttgatgaa ttctgcagtc agttccggca gttcacgctc ttcaactttc
    24961 ttcaggttga tagcgaattt cgctgcttta cctttcaggt tttctgcgtg gtattcttcc
    25021 gggaaggtca cgtcgatgga gaactcttcg ccagctttgt ggcctttgat accgtcttca
    25081 aagcccggga tcatacgacc ctggcccatc gccagtacga aatcagacgc tttaccgcct
    25141 tcgaactctt cgccgtctac agaaccggtg aagtcgatgg ttacgcggtc ttctgcttca
    25201 acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac gcagagtatc cagcatgccg
    25261 tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa cttcgatcgc ttccagaccc
    25321 tgcagttcaa cttccggata aacttcaaac tctacagagt aggtgaagtc ttcacccagc
    25381 ttgtattcgc ccggaacata agtcggtgcg ccagccggat tgattttttc tttaatgatg
    25441 gcgtcaatga agttacggct catcaggtca cccagaacgt cctggcgtac agacgcgcca
    25501 taacgctgag caacgatatt cattggcact ttgcctttgc ggaagccgtc aatacgtact
    25561 ttttttgcaa cgttgaccag ctcgctttta acagcggtct cgatgctgtc agcagcgata
    25621 gtaatcgtta cacggcggcc aaggccttga gtggtttcaa ctgaaacttg catcttgtta
    25681 cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca agcacaggtt gttggcttcg
    25741 cggaaccggg atgttcttat aatcaatcac attccctgtt accagaatca tcccgaaagc
    25801 attcaaatag gacgcggcat tatagcggca tcacttttat gagtcgagaa cggttattgc
    25861 gcgctgtcgc gtattttttc acaattcccg ggcaattttc gtctgaatgc tgggcaaaaa
    25921 gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt tttcataaaa tctttagcaa
    25981 catactggaa aagcgccgac agttgcaaat gcgtttttac gcgatgcttc ctgctccacg
    26041 acagggagga gaggcaaaga cggtgtcctg caacccttcc cactccttaa tagtgtaagt
    26101 atgcagagcc agcgcatgaa cggtagtaga gagttcctcc gctaaagtac tgtaaatcat
    26161 tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg ctgaccagca caactttaaa
    26221 atggctttca gagccggctg ggacattgtg acgatagctt tcatccacta cttcgaggaa
    26281 tacgggttgg aacgccgccc ttaatttttc ttctatccgc tcacgtatca tcatgaaata
    26341 tctcctccga caacgctgag atgtcatcca tccctttaaa tactagccgc ttttaccgtt
    26401 tccattgcag cttaacaaca aatatttagg tttcgtcctg gtttttccgt caagcgtatg
    26461 aagtttactg atttaaagat tgaaacacag gcctcgcatc agcgtttctt ttaccagggg
    26521 cgattatttc gtcaacatga tgaatttaca tgccttacct acttcccctc gccgttggca
    26581 atgttatgat ggcgggaatt tttaccaaac acgcttaaga gtctctgaga gcccgaacat
    26641 gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg cttgcaggat gcgcaaaacc
    26701 gccaacaact attgaagttt ccccgacgat tacactgcca cagcaggatc caagcctgat
    26761 gggcgtcacc gtaagcatta atggtgccga tcagcgtacc gatcaggcgc tggcaaaagt
    26821 cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc gatctgcgtt tcctgctgca
    26881 agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg gttggtccga atggcccggt
    26941 taatctgcaa atcatcgtta gccaactgta tgctgacgtg tcccagggca acgtgcgcta
    27001 caacatcgct accaaagcgg atatcgccat catcgctacc gcgcagaatg gcaacaaaat
    27061 gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg ttccaggcct ccaacaaaaa
    27121 tatcgccgat gcggttaaca gtgtgctgac cgataccatc gctgatatgt ctcaggacac
    27181 cagcattcac gaattcatca agcagaacgc gcgttaattt ctgccctctg gcccggtgca
    27241 agccgggcct gtagacgccc atgtccagtc aatatttacg tatttttcaa cagccgcgtt
    27301 cagccatatt gctgatcctg ggttttgctt ccgggctacc gctcgccctg acatccggca
    27361 ccttacaggc ctggatgacg gtcgagaata tcgatctcaa aaccattggt ttcttctctc
    27421 tggtaggcca ggcttacgtc tttaaattcc tctggtcacc gctgatggac cgctacacgc
    27481 ccccattttt tgggcggcgg cgcggttggc tgctcgccac gcaaatcctg ttattagtcg
    27541 ccattgcggc gatgggtttt ctcgaaccag gcacccaact ccgctggatg gcggcgctgg
    27601 cggtggtgat cgctttttgc tctgcctccc aggatattgt cttcgatgcg tggaaaaccg
    27661 atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat cagcgtgctg ggttaccgtt
    27721 tagggatgtt ggtttccggc ggcctagccc tgtggctggc agataaatgg ctgggctggc
    27781 agggcatgta ctggttgatg gcggcgctgt tgatcccctg tattatcgcg acgttgcttg
    27841 caccagaacc aaccgacacc attcctgtgc ccaaaacgct ggaacaagcg gttgttgcac
    27901 ctctgcgaga tttctttggt cgcaataatg cctggcttat tttgcttctt atcgtgctgt
    27961 ataagctggg cgacgcattc gccatgagcc tgacaaccac gtttttgatt cgcggcgtcg
    28021 ggtttgatgc gggtgaagta ggcgtggtta acaaaacact gggcttactg gcgaccattg
    28081 ttggcgcatt gtacggtggg attttgatgc agcgcctgtc actgttccgg gcactgctga
    28141 ttttcggcat tttacaaggt gcgtctaacg ctggttactg gctgctgtcg attactgata
    28201 agcatctcta cagcatgggc gcagccgtct ttttcgaaaa cctctgtggc gggatgggca
    28261 catcagcctt tgtcgcgctg ttaatgacgc tatgtaataa gtcattttcc gctactcaat
    28321 ttgccctgct ctcagcgctt tctgctgtag ggcgagttta tgtcggcccc gtggcgggtt
    28381 ggtttgttga agcacacggc tggtcgacat tctatctatt ctccgtcgcc gctgccgtac
    28441 cagggcttat tttgctgctg gtttgccgcc agacgcttga atatacacga gtaaatgaca
    28501 actttatctc ccgtaccgca tatccggcag gttatgcctt tgccatgtgg acactggcgg
    28561 cgggcgtcag cctgttggcc gtgtggttac tgctgttgac gatggacgcg ctggatttga
    28621 cgcacttctc tttcctgcct gctctgctgg aagtcggggt tttagtcgcc ctttctggcg
    28681 tcgtgcttgg tggtttgctg gattatctgg cgctacgaaa aacgcatctg acgtaatctg
    28741 taaatattat ttagctgtag taatcactcg ccgtaattat tacggctaaa taaacatcag
    28801 catcgcttat taatatttgt tcttttgtgc ggcttagcgt ttggaaatta ttggcgccat
    28861 ttataaattc tatttttctt acacgattca gctaatgagt ctttattttc tcacccccag
    28921 ttgtcactcc aatgataatt atttgttaaa taattgtttt atttcgtatt ggttatacca
    28981 attgcccgcc cagactcctt ctgcactccc cttttgttat aacgcccttt tgcaacagct
    29041 tcttaaaatc aacctgatat gttttgcaac atatgtgacc tgacagccaa atccaagtaa
    29101 caggaattta atcatgttta cagtaatgta accttcccgt aaaatgccca cacactttaa
    29161 acgccaccag atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag
    29221 tttgggatgg ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct
    29281 gttagatccc aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg
    29341 cctgatgttg attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta
    29401 ccgtgcgagc aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga
    29461 agctgtggtc tggacggtac ctatcttaat catcatcttc cttgcggtac tgacctggaa
    29521 aaccactcac gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat
    29581 cgaagtggtt tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgctac
    29641 cgtgaatgaa atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc
    29701 cgtgatgaac tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat
    29761 gcagactcgc ctgcatctga tcgccaacga ccccggcact tatgacggta tctccgccag
    29821 ctacagcggc ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc
    29881 cgcattcgac cagtgggtcg caaaagcgaa acagtcgccg aactccatgt ctgacatggc
    29941 agcgttcgaa aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt
    30001 gaaaccagac ttgtttgccg atgtaattaa caagtttatg gctcacggta agagcatgga
    30061 catgacccag ccagaaggtg agcacagcgc acacgaaggt atggaaggca tggacatgag
    30121 ccacgcggaa tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc
    30181 acttgatgca gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt
    30241 gggaggtctg gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa
    30301 agagtggctg acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat
    30361 tgtgatgttg ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc
    30421 ctcggcgggc gaagcgggct tcctgccacc tcaccactac gatcagatct ttaccgcgca
    30481 cggcgtgatt atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt
    30541 ggttccgctg cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt
    30601 ctggtttact gttgttggtg tgattctggt aaacgtttct ctcggcgtgg gcgaatttgc
    30661 gcagaccggc tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg
    30721 tgtcgattac tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat
    30781 caacttcttc gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc
    30841 agtatttacc tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct
    30901 gacggttacc gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa
    30961 cgatatgggt ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga
    31021 agtttacatc ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc
    31081 gcgtaaacgt ctgtttggtt atacctcgct ggtatgggca accgtctgta tcaccgtgct
    31141 gtcgttcatc gtttggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc
    31201 cttctttggt atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg
    31261 gctgttcacc atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggactatcgg
    31321 ttttatcgtc accttctcgg tgggcggtat gactggcgtg ctgctggcag taccgggcgc
    31381 ggacttcgtt ctacataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg
    31441 cggcgtggtc ttcggctgct tcgcagggat gacctactgg tggcctaaag cgttcggttt
    31501 caaactgaac gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt
    31561 tgcctttatg ccactgtacg cgctgggctt catgggcatg acccgtcgtt tgagccagca
    31621 gattgacccg cagttccaca ccatgctgat gattgcagcc agcggtgcag tactgattgc
    31681 gctaggtatt ctctgcctcg ttattcagat gtacgtttct attcgtgacc gcgaccagaa
    31741 ccgtgacctg actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc
    31801 acctccgttc tataactttg ccgttgtgcc gcacgttcac gagcgtgatg cattctggga
    31861 aatgaaagag aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc
    31921 gaaaaacagc ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat
    31981 gatctggcat atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat
    32041 cgtgaaaagc ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact
    32101 ggaaaaccag catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt
    32161 tgacgcacgc gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa
    32221 tcttcggatt ttggatctac ctgatgagcg actgcattct gttctctatc ttgtttgcta
    32281 cctatgccgt tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac
    32341 tgccgttcgt tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg
    32401 cggctatcgc catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct
    32461 ggttgtttgg cgccggattt atcgggatgg aaatctacga attccatcac ctgattgtta
    32521 acggcatggc tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc
    32581 acggtctgca cgtcacttcc ggtcttatct ggatggcggt gctgatggta caaatcgctc
    32641 gtcgcggcct gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact
    32701 tcctggatgt ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat
    32761 gagtcattct accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg
    32821 ctttatcctg tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc
    32881 ctctccggcc gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca
    32941 tctggtgtgc ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt
    33001 tgtcttcacc gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa
    33061 cctcaactac aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg
    33121 caagtaacga aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg
    33181 ctggcctcaa agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca
    33241 ctggttgtgg cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag
    33301 atggaaagga cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg
    33361 gtgtacgcca ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg
    33421 ctggcctgct ggctgggggt gatgggcttt gtggtttatg tcggcgttta tagcctgtac
    33481 atgaaacgcc actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg
    33541 gtgattggtt actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct
    33601 atcttcagcc tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat
    33661 taccaggcgg caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat
    33721 cacatcacgc tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt
    33781 tacgctggat ataaatatct ggtggtcgcc gcggcggtga gcgtctggtg gttaggtatg
    33841 gccctgcgcg gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc
    33901 tctatcatcg ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtgccggac
    33961 tcgcatacgc tgctggctgc tgtgtggtaa cacaacctct ctattaaaaa ggtgctacgg
    34021 cacctttttt cttagcatta gaaacatatc cctctcgaaa tatttactaa aaaatccgca
    34081 tgtttacccc attcgtttgc cgctttacac tagtcgcgaa tttaaaacag aggtggtaat
    34141 gaacgattat aaaatgacgc caggtgagcg gcgcgcgacc tggggtttag ggaccgtatt
    34201 ctcgttgcgc atgctgggca tgttcatggt tctgccggtt ctgaccacgt acggcatggc
    34261 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
    34321 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
    34381 tgtcggtggg ctggcagtgt ttgccgccgg tagcgttatc gctgcgctct ctgactccat
    34441 ctggggaatt attctgggtc gggcgctaca aggctccggt gcgattgccg ctgccgttat
    34501 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcaatgg cgtttatcgg
    34561 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
    34621 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacgaccg gcattgcgtt
    34681 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
    34741 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
    34801 tatgtgtctg catattttgc tgatgtcgac ctttgttgcc ctgcccggac aactggctga
    34861 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacaatgc taatcgcctt
    34921 tggctcggtc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
    34981 tgtcttctgc atcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cgcaaacgca
    35041 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
    35101 cctcctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
    35161 tgtttactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
    35221 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
    35281 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
    35341 cccggcgaac attgccgcaa acgaggcgtt aaaagtgcgt ttgctggaaa ctgaaggcat
    35401 caaagaagtg ttgattgcag aagaagaaca ttcagcttat gtgaaaatcg acagcaaagt
    35461 gacgaatcgc tttgaggttg aacaggcaat tcgccaggca taaaagcgag gcgaaattgc
    35521 ctgctgcact acgcttatca ggcctactaa gttcctgagt tacaaagctt tgtaggccga
    35581 atcaggcgtt catgccgcat ctgacatgaa caaagcgcag gttgtcgcga ttaatcgcgg
    35641 aagtttttga actggaacgg ctgacccaga tcgccaccgc gcaccatcgc catgacagcc
    35701 tgcaaatcat cacgagattt gccagtcacg cggatctcat cgccctgaat ttgcgcctgc
    35761 actttcagtt tgctgtcttt gatcatcttg acgattttct tctgggtcgc gctctcaatg
    35821 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
    35881 acatccagcg aactgccttc aatgccgcgc ttcagcagct tggcacgcaa aatatccagc
    35941 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
    36001 agctcaaatg aggcttcaac gttacggaag tcaaaacggg actccacttc gcggctcgcg
    36061 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
    36121 atcttttcct ctcccttcat ttttgatgcg aagcataata cctgcaaagt taaataacca
    36181 acagtcctgc accaggacgt atcccttaat tgtgcatagt cgcaacagca tgcacaatta
    36241 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
    36301 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
    36361 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
    36421 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
    36481 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
    36541 gtccacactg cctgtaacta cgccaatact gttaattcac aacggcatgg gcaccatcga
    36601 agaattgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
    36661 cgacggcaat gtcattattc atgtggcaaa cggtatcacg catattggtc cagcacggca
    36721 acaggacggc gattacagtt atctggcgga tattttgcaa accgtgttgc ctgacgttgc
    36781 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gcagtcaact gtgtgattaa
    36841 tccactgact gccatctgga attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat
    36901 tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc gaagggcatc atacttcagc
    36961 ggaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
    37021 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
    37081 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
    37141 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
    37201 gtgaagagac tgaagccgtc accacgatcg acctcttggt tcgcggcggt atcaaagtca
    37261 ccactgccag cgtagccagc gatggcaacc tggcgattac ctgctcgcgc ggcgtgaagc
    37321 tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga atatgacgtg atcgtactgc
    37381 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
    37441 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
    37501 tggtcccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
    37561 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
    37621 tgaccagcca ggggccgggt acagctatcg actttggtct gaaaattatc gacctgttgg
    37681 ttgggcgtga aaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
    37741 attattacga gtagtgtcgg atgcggtaag cgtcgcatct gaccagatgc gacgttacaa
    37801 ccacaaatta cgggcgatat accttcacat tgttaaagcc ctgctcgcgc agatagagcg
    37861 cctgcagacg gctcatcacc ccgcgctcac accacaacag ccaggtttta ctctggtcga
    37921 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacatca accccttcga
    37981 ctttcagtgg cttatcttct tgttcatcga tagaacggat atcgaggatg acgtcattcg
    38041 ggccgaagcc attgacagtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
    38101 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatgctg aagtcgaact
    38161 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
    38221 caccgcagta ttccggcatc gtgcgagcaa agtcttcggt gccaatctgg cgagccaggt
    38281 tgatgatgtg ctctttgtcg taagagatca gcggacgcag gatcagcgta tcagagacgt
    38341 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
    38401 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
    38461 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
    38521 gctcgaaatt aatagcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
    38581 aatgcgccac ctgacgtacg ccaatttcat gcgccgcgcc gccgaggtta aagaagcagt
    38641 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
    38701 cggaaatgag cgacagtaca tcttcctggg tgccgatcgg gaaaccacca ataccttcgt
    38761 agcggccttt aatcagcagg agacgatcgt cttccacttc caaatggaca gtcacatccg
    38821 gattggtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
    38881 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
    38941 ttttgccttc cagctgatcg cgatactgaa ccaacgcttt ctcgaaaata tcgtgcatgt
    39001 cggtaaacgg cacatcttcg acttcgagaa tatggtggat acccggaata cgggtcagag
    39061 cgtcgcgaat agccagacgc tggttttcat cttttgcgcg aacttcgatg ttatcccagt
    39121 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
    39181 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
    39241 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
    39301 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
    39361 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
    39421 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
    39481 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
    39541 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
    39601 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
    39661 atgaagacgc ctctctaacc ccttttacac cggacaatga gtaatggact ttccgcagca
    39721 actcgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
    39781 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
    39841 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
    39901 ggacgcaccc gctgctgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
    39961 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
    40021 cgaagcgaac gcgattctcg ctggcgatgc tttacaaacg ctggcgttct cgattctaag
    40081 cgatgctgat atgccggaag tgtcggatcg cgacagaatt tcgatgattt ctgaactggc
    40141 gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag acgcggaagg
    40201 caaacatgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
    40261 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
    40321 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
    40381 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
    40441 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
    40501 gatcgacgat gcccgccagt cgctgaaaca actggctgaa cagtcactcg atacctcggc
    40561 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
    40621 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
    40681 ggagttacga ctgttgccaa aagagagttt accgaaactc tgcgacgaac tgcgccgcta
    40741 tttactcgac agcgtgagcc gttccagtgg gcacttcgcc tccgggctgg gcacggtcga
    40801 gctgaccgtg gcgctgcact acgtctataa cactccgttt gaccagttaa tctgggatgt
    40861 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
    40921 ccgtcagaaa ggtggcctgc acccgttccc gtggcgcggc gaaagcgaat atgacgtatt
    40981 aagcgtcgga cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttgctgccga
    41041 gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc gatggcgcga ttaccgcagg
    41101 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggttct
    41161 caacgacaat gaaatgtcga tttccgaaaa tgtcggtgcg ctcaacaacc atctggcaca
    41221 gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag ttttctctgg
    41281 cgttccgccc attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtggt
    41341 gcctggcaca ttgtttgaag agctgggctt taactacatc ggcccggtgg acggtcacga
    41401 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagtttct
    41461 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc caatcacctt
    41521 ccacgctgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggtggttt
    41581 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagataacaa
    41641 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
    41701 attcccggat cgctacttcg atgtggcaat tgccgagcaa cacgcggtga cctttgccgc
    41761 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
    41821 cgcctatgat caggtgttgc atgacgtggc gattcaaaag cttccggtcc tgttcgccat
    41881 cgaccgtgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgatctctc
    41941 ttatctgcgc tgcataccgg aaatggtcat tatgactccg agcgatgaaa acgaatgtcg
    42001 cctgatgctc tataccggct atcactataa cgatggcccg tcagcggtgc gctacccgcg
    42061 tggcaacgcg gtcggcgtgg aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat
    42121 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagaagc
    42181 ggcgaaagtc gccgaatcgc tgaacgccac gctggtcgat atgcgttttg tgaaaccgct
    42241 tgatgaaacg ttaattctgg aaatggccgc cagtcatgaa gcactggtta ccgtagaaga
    42301 aaacgccatt atgggcggtg caggcagcgg agtgaacgaa gtgctgatgg cccatcgtaa
    42361 accagtaccc gtgctgaaca ttggcctgcc ggacttcttt attccgcaag gaactcagga
    42421 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
    42481 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
    42541 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
    42601 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
    42661 cagccgtccc ataattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
    42721 tagctattcc gatggcagca gcgaagagat cgtcggtcgc gcactgcggg atttctcccg
    42781 tcgtgaagac gtggtcgttg ccaccaaagt gttccatcgc gttggtgatt taccggaagg
    42841 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgacgtc tcggcatgga
    42901 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
    42961 ggaagccctc aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatcaat
    43021 gcacgcttcg cagtttgctc aggcgctgga gctacaaaaa cagcacggct gggcgcagtt
    43081 tgtcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
    43141 gctgtgttat caggaaggcg tggcggtgat tccgtggagc ccgctggcac ggggacgtct
    43201 gacgcgtccg tggggagaaa ccactgcacg actggtgtct gatgaggtgg ggaaaaatct
    43261 ctataaagaa agcgatgaga atgacgcgca aattgcggag cggttaacgg gcgtcagtga
    43321 agatctgggc gcgacacgag cacaagtcgc actggcatgg ttgttgagca aacctggcat
    43381 tgctgcaccg attatcggta catcgcggga agaacagctt gatgagctac tgaacgcggt
    43441 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatcc
    43501 ggtcgtagga tttaagtaaa tttcgctttt cgcccatttt atctgctgaa ttccccatcg
    43561 tctgcgatga tggggacaac gtacctccca tcaccttctg taaccataat ccacctctgc
    43621 aagagaaatt cttcgtgatt aaatttacag ctattcatca tcttcatcgc tttgagcgat
    43681 ttttgatagt ttctcccgat aatatcccct aaaaatgtct tatttagtca atgtaagagg
    43741 attacaatca tgatgaaaac catcaccaaa caaccgatac tgtttactga tgtaccggtt
    43801 gcagatttaa gaaactcaat gaagcaagat ttaaatcaaa atcttattga aaggctgtgg
    43861 aacaaaattc gtgatttttt tctggacagt gataaacaaa aggcatttaa atc
//
LOCUS       DAZGBC010000018        43293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGBC010000018 DAZGBC010000000
VERSION     DAZGBC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.018"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..183)
                     /locus_tag="ACHK1J_001050"
     tRNA            complement(107..183)
                     /locus_tag="ACHK1J_001050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:Arg,seq:acg)
     gene            complement(382..458)
                     /locus_tag="ACHK1J_001051"
     tRNA            complement(382..458)
                     /locus_tag="ACHK1J_001051"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(422..424),aa:Arg,seq:acg)
     gene            complement(521..597)
                     /locus_tag="ACHK1J_001052"
     tRNA            complement(521..597)
                     /locus_tag="ACHK1J_001052"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Arg,seq:acg)
     gene            complement(797..873)
                     /locus_tag="ACHK1J_001053"
     tRNA            complement(797..873)
                     /locus_tag="ACHK1J_001053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Arg,seq:acg)
     gene            complement(877..969)
                     /locus_tag="ACHK1J_001054"
     tRNA            complement(877..969)
                     /locus_tag="ACHK1J_001054"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(933..935),aa:Ser,seq:gct)
     gene            complement(1285..1470)
                     /gene="csrA"
                     /locus_tag="ACHK1J_001055"
     CDS             complement(1285..1470)
                     /gene="csrA"
                     /locus_tag="ACHK1J_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO7910362.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1705..4335)
                     /gene="alaS"
                     /locus_tag="ACHK1J_001056"
     CDS             complement(1705..4335)
                     /gene="alaS"
                     /locus_tag="ACHK1J_001056"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO7910363.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4463..4963)
                     /gene="recX"
                     /locus_tag="ACHK1J_001057"
     CDS             complement(4463..4963)
                     /gene="recX"
                     /locus_tag="ACHK1J_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO7910364.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATEEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLSTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(5200..6261)
                     /gene="recA"
                     /locus_tag="ACHK1J_001058"
     CDS             complement(5200..6261)
                     /gene="recA"
                     /locus_tag="ACHK1J_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO7910365.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6341..6838)
                     /gene="pncC"
                     /locus_tag="ACHK1J_001059"
     CDS             complement(6341..6838)
                     /gene="pncC"
                     /locus_tag="ACHK1J_001059"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO7910366.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6983..8068)
                     /gene="mltB"
                     /locus_tag="ACHK1J_001060"
     CDS             complement(6983..8068)
                     /gene="mltB"
                     /locus_tag="ACHK1J_001060"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO7910367.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8324..8887
                     /gene="srlA"
                     /locus_tag="ACHK1J_001061"
                     /gene_synonym="gutA"
     CDS             8324..8887
                     /gene="srlA"
                     /locus_tag="ACHK1J_001061"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO7910368.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8884..9843
                     /gene="srlE"
                     /locus_tag="ACHK1J_001062"
                     /gene_synonym="gutE"
     CDS             8884..9843
                     /gene="srlE"
                     /locus_tag="ACHK1J_001062"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO7910369.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLVQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9854..10225
                     /gene="srlB"
                     /locus_tag="ACHK1J_001063"
                     /gene_synonym="gutB"
     CDS             9854..10225
                     /gene="srlB"
                     /locus_tag="ACHK1J_001063"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO7910370.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            10229..11008
                     /gene="srlD"
                     /locus_tag="ACHK1J_001064"
     CDS             10229..11008
                     /gene="srlD"
                     /locus_tag="ACHK1J_001064"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO7910371.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            11114..11473
                     /gene="gutM"
                     /locus_tag="ACHK1J_001065"
     CDS             11114..11473
                     /gene="gutM"
                     /locus_tag="ACHK1J_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO7910372.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11540..12313
                     /gene="srlR"
                     /locus_tag="ACHK1J_001066"
                     /gene_synonym="gutR"
     CDS             11540..12313
                     /gene="srlR"
                     /locus_tag="ACHK1J_001066"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO7910373.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            12306..13271
                     /gene="gutQ"
                     /locus_tag="ACHK1J_001067"
     CDS             12306..13271
                     /gene="gutQ"
                     /locus_tag="ACHK1J_001067"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO7910374.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(13268..14782)
                     /gene="norR"
                     /locus_tag="ACHK1J_001068"
     CDS             complement(13268..14782)
                     /gene="norR"
                     /locus_tag="ACHK1J_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO7910375.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAARFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14969..16408
                     /gene="norV"
                     /locus_tag="ACHK1J_001069"
     CDS             14969..16408
                     /gene="norV"
                     /locus_tag="ACHK1J_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO7910376.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            16405..17538
                     /gene="norW"
                     /locus_tag="ACHK1J_001070"
     CDS             16405..17538
                     /gene="norW"
                     /locus_tag="ACHK1J_001070"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO7910377.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVMNIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(17764..20016)
                     /gene="hypF"
                     /locus_tag="ACHK1J_001071"
     CDS             complement(17764..20016)
                     /gene="hypF"
                     /locus_tag="ACHK1J_001071"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO7910378.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRVFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLTTFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(20169..20696)
                     /gene="hydN"
                     /locus_tag="ACHK1J_001072"
     CDS             complement(20169..20696)
                     /gene="hydN"
                     /locus_tag="ACHK1J_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO7910379.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20846..21856)
                     /gene="ascG"
                     /locus_tag="ACHK1J_001073"
     CDS             complement(20846..21856)
                     /gene="ascG"
                     /locus_tag="ACHK1J_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO7910380.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVMGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            22116..23573
                     /gene="ascF"
                     /locus_tag="ACHK1J_001074"
     CDS             22116..23573
                     /gene="ascF"
                     /locus_tag="ACHK1J_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO7910381.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEIVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAISGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            23582..25006
                     /gene="ascB"
                     /locus_tag="ACHK1J_001075"
     CDS             23582..25006
                     /gene="ascB"
                     /locus_tag="ACHK1J_001075"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO7910382.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(25165..25635)
                     /gene="hycI"
                     /locus_tag="ACHK1J_001076"
     CDS             complement(25165..25635)
                     /gene="hycI"
                     /locus_tag="ACHK1J_001076"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO7910383.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(25628..26038)
                     /gene="hycH"
                     /locus_tag="ACHK1J_001077"
     CDS             complement(25628..26038)
                     /gene="hycH"
                     /locus_tag="ACHK1J_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO7910384.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26035..26802)
                     /gene="hycG"
                     /locus_tag="ACHK1J_001078"
     CDS             complement(26035..26802)
                     /gene="hycG"
                     /locus_tag="ACHK1J_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO7910385.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGNMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26802..27344)
                     /gene="hycF"
                     /locus_tag="ACHK1J_001079"
     CDS             complement(26802..27344)
                     /gene="hycF"
                     /locus_tag="ACHK1J_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO7910386.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(27354..29063)
                     /gene="hycE"
                     /locus_tag="ACHK1J_001080"
     CDS             complement(27354..29063)
                     /gene="hycE"
                     /locus_tag="ACHK1J_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO7910387.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(29081..30004)
                     /gene="hycD"
                     /locus_tag="ACHK1J_001081"
     CDS             complement(29081..30004)
                     /gene="hycD"
                     /locus_tag="ACHK1J_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO7910388.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(30007..31833)
                     /gene="hycC"
                     /locus_tag="ACHK1J_001082"
     CDS             complement(30007..31833)
                     /gene="hycC"
                     /locus_tag="ACHK1J_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO7910389.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31830..32441)
                     /gene="hycB"
                     /locus_tag="ACHK1J_001083"
     CDS             complement(31830..32441)
                     /gene="hycB"
                     /locus_tag="ACHK1J_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO7910390.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(32566..33027)
                     /gene="hycA"
                     /locus_tag="ACHK1J_001084"
     CDS             complement(32566..33027)
                     /gene="hycA"
                     /locus_tag="ACHK1J_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO7910391.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33238..33588
                     /gene="hypA"
                     /locus_tag="ACHK1J_001085"
     CDS             33238..33588
                     /gene="hypA"
                     /locus_tag="ACHK1J_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7910392.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            33592..34464
                     /gene="hypB"
                     /locus_tag="ACHK1J_001086"
     CDS             33592..34464
                     /gene="hypB"
                     /locus_tag="ACHK1J_001086"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO7910393.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     TPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            34455..34727
                     /gene="hypC"
                     /locus_tag="ACHK1J_001087"
     CDS             34455..34727
                     /gene="hypC"
                     /locus_tag="ACHK1J_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO7910394.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34727..35848
                     /gene="hypD"
                     /locus_tag="ACHK1J_001088"
     CDS             34727..35848
                     /gene="hypD"
                     /locus_tag="ACHK1J_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO7910395.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35845..36855
                     /gene="hypE"
                     /locus_tag="ACHK1J_001089"
     CDS             35845..36855
                     /gene="hypE"
                     /locus_tag="ACHK1J_001089"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO7910396.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLIAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLASLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36929..39007
                     /gene="flhA"
                     /locus_tag="ACHK1J_001090"
     CDS             36929..39007
                     /gene="flhA"
                     /locus_tag="ACHK1J_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO7910397.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            39052..39480
                     /locus_tag="ACHK1J_001091"
     CDS             39052..39480
                     /locus_tag="ACHK1J_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO7910398.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(39519..39863)
                     /gene="ygbA"
                     /locus_tag="ACHK1J_001092"
     CDS             complement(39519..39863)
                     /gene="ygbA"
                     /locus_tag="ACHK1J_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO7910399.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(39940..40074)
                     /locus_tag="ACHK1J_001093"
                     /pseudo
     CDS             complement(39940..40074)
                     /locus_tag="ACHK1J_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40150..42711
                     /gene="mutS"
                     /locus_tag="ACHK1J_001094"
     CDS             40150..42711
                     /gene="mutS"
                     /locus_tag="ACHK1J_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO7910400.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIAPGYNEELDEWRALADGATDYLERL
                     EIRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42817..>43293
                     /locus_tag="ACHK1J_001095"
     CDS             42817..>43293
                     /locus_tag="ACHK1J_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO7910401.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FELNDMEQKEAIGLLLKFHQLPHIIEITNDIIKYVIAHADYPGNEYLFGKQIAESEL"
BASE COUNT        10174 a        11896 c        11374 g         9849 t
ORIGIN      
        1 cggctcgccc ttcgggccgt tgctaaagca acgttatcct ccctggtgcc tgcggttaat
       61 accgctacct cttcgatatc ttcattgctg aagatttcgg ataatatggt gcatccggga
      121 ggattcgaac ctccgaccgc tcggttcgta gccgagtact ctatccagct gagctacgga
      181 tgcatcggga aaatttattt tactgctgat attgatatcg ctactaaagc catatcaagt
      241 aagagatggt gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgctg
      301 gcgcaacgtt atcctccctg gtgcctgcgg ttaattccgc tacctcttcg atatcttcat
      361 tgctgaagat ttcggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt
      421 tcgtagccga gtactctatc cagctgagct acggatgcat cgggaaattt attttactgc
      481 tgatattgat accgctacta aagccatatc aagtaagaga tggtgcatcc gggaggattc
      541 gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc
      601 gggaaactta ctttactgca gattttttga taccgctact aaagccgtat caagtaagag
      661 atggtgcatc cgggaggatt actcgccttg cggctcgccc ttcgggccgt tgctaaagca
      721 acgttatcct ccctggtgcc tgcggttagt accgccacct cttccatacc ttcatcgctg
      781 aaggttacgg agaatatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
      841 gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg ggattcgaac
      901 cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca gcctctcggc
      961 cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct catcgtcgct
     1021 gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc cacactttta
     1081 tcgtttgcac acttcacgtt caattagtct gtaaaaacgt caaaaagagt gttttatcaa
     1141 cagaaaaatg gaggtctgac agatagcagt aatgtaaaaa aatggagact taagttgaat
     1201 gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg tgcgtctcac
     1261 cgataaagat gagacgcgga aagattagta actggactgc tgggattttt cagcctggat
     1321 acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta cgccaatacg
     1381 tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa tcatgagggt
     1441 ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt aaaagagtcg
     1501 ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc aatgacaaac
     1561 acattacatc taagcagtca tggcattaca ttctgttaaa cctaagttta gccgatatac
     1621 acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg tgaagcgagt
     1681 ccacggcatt gccttacgct tatattattg caatttcgcg ctgacccagc ctttcacact
     1741 ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca tgtcaggacg
     1801 tccaccaccc ttgccaccca cctgctgagc gaccatacca atcagttccc ctgctttcac
     1861 acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt cggctaccgt
     1921 tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca tggtacgcaa
     1981 cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat taacatcaat
     2041 tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa gctgttgtag
     2101 ttctttttct aactgacgcg tacgttccag tactgagcgc actttatcag ccagattatt
     2161 gctatcgcct ttcagcagat gtgcgacttc gcttaagcga tcgctgtctg catgaacggt
     2221 ggcgatagcg ccttctccgg ttaccgcttc aatacgacga acgcctgcag cagtacccga
     2281 ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag taccgccaca
     2341 cagctcggtg gagaaatcgc ccatgctcag cacgcgtacg cgctcatcat acttctcgcc
     2401 gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga tccatgatgt tggtttcgat
     2461 cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa tctcttctgg
     2521 tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa ccagtgaacc
     2581 tttctgcgat acatgagtac ccagaacctg gcgcagcgca gcatgcatca ggtgcgttgc
     2641 ggagtgattc agacgaatac gggcgcgacg agcctcatca acatcagcct gcacagcgtc
     2701 gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct ggccgtattt
     2761 ctgcgtatct tccaccacga aggagaagtt agcgcctttc agttcgcctt tatcaccaac
     2821 ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag cttcctggcc
     2881 tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt tgccgttcag
     2941 ttccagatgg tcatagcctt taaattcaga tgcactgtca acacggatca ttgcgttgta
     3001 atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca ttgcagcttc
     3061 aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg tcaggtcaac
     3121 cgggaagcca taggtgtcgt acagacggaa agcagtttca ccatccagcg tatcaccaga
     3181 aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac gagcaaactg
     3241 ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca ggtcttcacc
     3301 cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg tttctttcgc
     3361 gccgagcata ttaccgtgac gcactgcgcg acgaatgata cgacgcagta cataaccacg
     3421 gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac gaatgtggtc
     3481 agcgattacg cgcagcgatt tattgctcag atcggttgcg ccagtgactt tcgctaccgc
     3541 ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt gcagcaccgc
     3601 agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg gttccatcgt
     3661 gccatcggcc tggcggttga actgcatgaa gacgatgttc cagatctcaa tgtagcggtc
     3721 gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt gatcgtagaa
     3781 gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt tgtcagatgc
     3841 gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga tcccgacttc
     3901 tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc acagacgctc
     3961 tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact gaatggcatc
     4021 gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg tatggtgacg
     4081 cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca cacagcgttg
     4141 ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat ccttgaactg
     4201 gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca gggagctgct
     4261 ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac ggatctcagc
     4321 ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca ccagctccag
     4381 cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt ttcttgactg
     4441 ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc tggatatctt
     4501 ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct gaaaagacag
     4561 ttgacaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg caccagtcga
     4621 tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc tgattcagtt
     4681 cctggcgaat acgcgcaggt ccataacctt tgcggctacg gctggcgata aagcgcgcaa
     4741 caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg cgctcgtaat
     4801 cttcttccgt ggcatcaatc tcttccgggc catttttgcc cataatcggt gccgcgagtt
     4861 tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc gcacgatcca
     4921 acaggcgagc atatgccggg cgacgggacg ttgattctgt catggcatat ccttacaact
     4981 taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag atttaatagt
     5041 caatgagtgt gcagaatgtt cccgcaggcc caattcgtta gaggatcgag gtgagagtaa
     5101 tttttcacgc cagtgctggc ctgttcccct caccctctcc ccaaaggggc gaggggaccg
     5161 tccgtgcaca cattatctat tatcatcaat aacgaacgat taaaaatctt cgttagtttc
     5221 tgctacgcct tcgctatcat ctacagagaa atccggcgtt gagttcgggt tgctcagcag
     5281 caactcacgt actttcttct cgatctcttt cgcggtttcc gggttatctt tcagccaggc
     5341 agttgcattc gctttaccct gaccgatctt ctcacctttg tagctgtacc acgcgcctgc
     5401 tttctcgatc agcttctctt ttacgcccag gtcaaccagt tcgccgtaga agttgatacc
     5461 ttcgccgtag aggatctgga attcagcctg tttaaacggc gcagcgattt tgttcttcac
     5521 cactttcacg cgggtttcgc tacccaccac gttttcgccc tctttcaccg cgccgatacg
     5581 acggatgtcg agacgaacag aggcgtagaa tttcagcgcg ttaccaccgg tagtggtttc
     5641 cgggttaccg aacatcacac caattttcat acggatctgg ttgatgaaga tcagcagcgt
     5701 gttggactgc ttcaggttac ccgccagctt acgcatcgcc tggctcatca tacgtgccgc
     5761 aaggcccatg tgagagtcgc cgatttcgcc ttcgatttcc gctttcggcg tcagtgccgc
     5821 cacggagtca acgacgataa cgtctactgc gccagaacgc gccagggcgt cacagatttc
     5881 cagtgcctgc tcgccggtgt ccggctggga gcacagcagg ttgtcgatat cgacgcccag
     5941 tttacgtgcg tagattgggt ccagcgcgtg ttcagcatcg ataaacgcac aggttttacc
     6001 ttcacgctgc gctgcggcga tcacctgaag cgtcagcgtg gttttaccgg aagattccgg
     6061 tccgtagatt tcgacgatac ggcccatcgg cagaccacct gccccaagcg cgatatccag
     6121 tgaaagcgaa ccggtagaga tggtttccac atccatggaa cggtcttcac ccaggcgcat
     6181 gatggagcct ttaccaaatt gtttctcaat ctggcccagt gctgccgcca acgctttctg
     6241 tttgttttcg tcgatagcca tttttactcc tgtcatgccg ggtaataccg gatagtcaat
     6301 atgttctgtt gaagcaatta tactgtatgc tcatacagta tcaagtgttt tgtagaaatt
     6361 gttgccacaa ggtctgcaat gcatacgcag tagcctgacg acgcaccgca tcacggtcgc
     6421 cgctgaagca ttcccgccgg gtaatgcctt caccgcgggc agtggcaaaa gcaaaccaga
     6481 cggtgccgac aggcttctct tcactgccgc catccggccc ggcgatacca ctaatagaca
     6541 cggcgtaatc agcacgagcc gctttcagtg cgcctatcgc catttccacc acgacgggtt
     6601 cgctcaccgc gccatgctgc gccagcgtct cttcacgtac gccgatcatc tgcgctttgg
     6661 cttcgttact gtaggtgaca aatccgcgtt caaaccaggc ggagctaccg gcaatatcgg
     6721 taatcacttt cgctacccaa ccgccggtac aagactcagc agttgttacg gttgcgccac
     6781 gggctttcag cgcctgccca acctgttcgc ttaactgcat cagttcactg tcagtcatca
     6841 caacctctgt cagtcaaaaa tttatgccgg acaagatagc actttcgtca ggaagatggt
     6901 gttggcgttg caattttacg aggaggattc agaaaaaagc tgattagcca gagggaagct
     6961 cacgctcccc tcttgcaaat agttactgca cgcgcgccag cgccacggct tgtcctaact
     7021 gccagaccgc cattgcgtaa tgggtgctgt ggttataacg ggtaatggtg tagaagttcg
     7081 gcagaccgta ccagtactgg taaccggtac cgacatccag acgcagcagg ctggcttgtt
     7141 gatggttgcc cagcggctgc tgtggcgtta aacctgccgc ggcaagctgc gaaatgctgt
     7201 acttggtttt gaagccattt ggcaagcctg gagcctgacc gtttgccatt accgcgacct
     7261 gatcgccttt cacccaaccg tgcgctttga aataattcgc cacgctaccg atcgcatcaa
     7321 ccggatccca caggttgata tgcccgtcgc cgctgaaatc taccgcatat tgtttgtaag
     7381 acgacggcat aaactgtccg tagcccatcg ccccggcaaa ggaacctttc agattgagcg
     7441 gatcgtcctg ctcgtcgcgc gccatcagca ggaaggtttc cagctcgcca gagaaatact
     7501 ccgcgcggcg tgggtagtta aatgacagcg ttgccagcgc atcgaggatg cgagttttcc
     7561 ccatcacgcg cccccagcgg gtttcaacgc cgataatccc gacgataatt tccggcggta
     7621 cgccatacac ctgccacgcg cgattcaacg catcttcata ctgattccag aaaaccacac
     7681 cgttctgcac gttgtccggc gtaataaatt ttttgcgata acgcagccat gcgccgttcg
     7741 gacctgatgg cggtttcacc gatgtggttg gtgcctggtt atccatcagc cgcagtaccg
     7801 aatccagacg tttcgcctgg gagagaattt cctgcaactg ctgacgatcg aaaccgtgtt
     7861 tgttcaccat tttgtcgatg aactgctggg cattcgggtt attggcgaag tcaccgccca
     7921 tctgcatcac attgtgctgc ggctcaagca ggaagccgcc agacggcgtt ccggtggtcg
     7981 tttcagtctc agtaggtttt ggcttgctgc tacaggcggc aagcaacaca aaaaggggaa
     8041 gcaatgttac ataacgacgc ttgaacatga ggggtccatt taacagattc aaccaggggc
     8101 aagtatggta aagcatcacg ccccgcacaa ggaagcggta gtcactgccc gatacggact
     8161 ttacataact caactcatcc ccctcgctat ccttttattc aaactttcaa attaaaatat
     8221 ttatctttca ttttgcgatc aaaataacac ttttaaatct ttcaatctga ttagattagg
     8281 ttgccgtttg gtaataaaac aataaatcct gaaggagaga acaatgatag aaaccattac
     8341 tcatggtgca gagtggttta tcgggctgtt ccaaaagggc ggagaggtgt ttaccgggat
     8401 ggtgaccggc attcttccgc tgttgattag cctgctggtt atcatgaacg cactgattaa
     8461 ttttatcggt cagcatcgaa ttgaacgttt tgctcaacgt tgcgccggta accctgtttc
     8521 ccgttacctg ctgctaccgt gcattggcac gtttgtcttt tgcaatccga tgaccctaag
     8581 tctgggtcgc tttatgccgg aaaagtacaa acccagctac tacgcggccg cctcttatag
     8641 ctgccactca atgaatggcc tcttccccca tatcaaccct ggcgaactgt ttgtttatct
     8701 tggcattgcc agcggtctga caacgctgaa cctgccactt ggcccactgg cggtgagcta
     8761 tctgctggtt ggtctggtca ccaacttctt ccgcggctgg gtgaccgatc tgaccaccgc
     8821 catttttgag aaaaagatgg gtattcaact tgaacaaaaa gttcacctgg caggagcaac
     8881 atcatgacgc gtattcggat cgaaaaagga acgggtggct ggggcggccc gcttgagctg
     8941 gaagccacgc cgggcaaaaa aatcgtctat atcaccgccg gtacccggcc tgcgattgtt
     9001 gacaaactgg tacagcttac tggctggcag gctattgacg gatttaaaga aggtgaaccc
     9061 gcggaggcgg aaattggtgt cgcggtaatc gactgtggcg gcacattacg ctgcggcatt
     9121 tatccgaaac ggcgtattcc caccattaat atccactcga cgggcaagtc cggtccactg
     9181 gcgcagtaca ttgtggaaga tatttatgtc tctggcgtaa aagaagaaaa catcactgta
     9241 gtgggtgatg cgacaccaca accctcttcc gtgggccgtg actatgacac cagcaagaaa
     9301 atcaccgaac aaagcgatgg tttactggcg aaggtgggaa tgggtatggg ttctgccgtt
     9361 gccgtgttgt ttcaatctgg tcgtgacacc atcgacactg tattaaaaac cattctgccg
     9421 tttatggcat tcgtctcggc gctcattggc atcattatgg cttctggcct tggtgactgg
     9481 attgcccacg gtcttgctcc gctggcgagc catccactgg gtctggtcat gctggcgctc
     9541 atctgctcct tcccgctgct ttcacctttc ctcggcccag gcgcagttat cgcacaggtt
     9601 atcggcgtat tgattggcgt gcagattggt ctcggcaata ttccgccgca tctggcttta
     9661 cctgcgctgt ttgccatcaa cgcgcaggcg gcctgcgact tcatcccggt cggtttgtcg
     9721 ctggcggaag cccgtcagga cacggttcgc gtcggtgtcc cttctgttct ggtgagccgc
     9781 tttttaaccg gcgcaccgac tgtactgatc gcctggtttg tctccggttt tatctatcaa
     9841 tagaggctga aacatgaccg ttatttatca gaccaccatc acccgtatcg gcgcgagtgc
     9901 cactgacgcc ctcagcgacc agatgctcat cacctttcgt gaaggcgcgc ctgcggatct
     9961 cgaagagtat tgcttcattc attgccacgg cgagttgaaa ggtgcactcc atcccggttt
    10021 gcaattttcg ctcgggcagc atcgctatcc ggtgaccgcc gttggcagcg tggcggaaga
    10081 caaccttcgc gaactgggtc atgtcaccct gcgcttcgat ggtttaaacg aagcggaatt
    10141 tccgggcact gtccatgtgg caggccctgt ccccgacgat atcgcgccgg gatcggtttt
    10201 gaagtttgaa tctgttaagg agtaaaaaat gaatcaggtt gccgttgtca tcggtggtgg
    10261 gcaaacctta ggcgcgttcc tgtgccacgg tctggctgcc gaggggtatc gcgtcgcggt
    10321 tgtcgatatt cagagcgaca aagccgcaaa tgtggcacaa gaaattaacg ccgaatatgg
    10381 tgaaggtatg gcgtacggtt ttggtgctga cgccaccagt gagcaaagcg ttctggcgct
    10441 ctctcgtggg gtagatgaaa tctttggtcg cgtggatttg ctggtctaca gcgccggaat
    10501 tgcaaaagca gcctttatca gcgacttcca gctcggtgat tttgaccgtt cgctacaggt
    10561 gaatctggtg ggttatttcc tgtgtgcgcg tgaattttcg cgtttgatga tccgcgatgg
    10621 gattcaggga cgcattattc agatcaactc gaaatccggc aaagtgggca gcaaacacaa
    10681 ctctggctac agcgcagcga aatttggtgg cgtagggctg actcaatcac tggcgctgga
    10741 tctggcggag tacggcatta cggtgcattc actgatgctc ggtaacctgc tgaaatcgcc
    10801 gatgttccag tcgctgttgc cacaatacgc gaccaagctg ggtatcaaac cggatcaagt
    10861 cgagcagtat tacatcgata aagtaccgct caaacgcggc tgcgattatc aggatgtgct
    10921 gaatatgctg ctgttctacg ccagtcctaa ggcatcgtac tgcactggtc agtcgatcaa
    10981 tgtcaccggc ggtcaggtga tgttctgatc aacagcggag atccgttaag gatctccgtg
    11041 ggactaatag aatgcctgat gcgctacgct catcaggcat acaggacttc cgccactaca
    11101 ttaaggaaaa gttatggtat ccgcactcat cactgtcgcc gttatcgcct ggtgtgcgca
    11161 actggcctta ggcggctggc aaatttctcg ttttaaccgt gccttcgaca cactatgcca
    11221 gcaagggcgg gttggcgtgg gccgttccag cgggcgcttt aaaccgcggg tcgtggtcgc
    11281 catcgccctg gacgatcagc agcgcatcgc cgacaccttg tttatgaaag gactgaccgt
    11341 cttcgcccga ccgcaaaaaa ttcccgcaat taccggcatg catgcgggtg atttacagcc
    11401 cgatgtgatc tttccccatg atccactatc acagaatgct ctatcattgg cgcttaaact
    11461 gaaacgtgga taatttcgtt gtgaatgtta cttgcttgcg aagttatcat tttgaaacct
    11521 aaatcaggta atcacgccca tgaaacctcg tcagcgtcag gccgccattc tggagtatct
    11581 gcaaaagcag ggtaaatgct cggttgaaga attggcgcaa tactttgaca ccacaggcac
    11641 aaccattcgc aaagatctgg tcattctgga acatgccgga accgtcattc gtacttatgg
    11701 cggagtggtg ttgaataaag aggaatccga tccgcctatc gatcataaaa cactcatcaa
    11761 cacccacaag aaagagctga tagcagaagc tgccgttggt tttattcatg atggcgattc
    11821 gatcattctt gatgctggca gcaccgtttt gcagatggtg cctctgctct cgcgctttaa
    11881 taacatcacg gtgatgacca acagcctgca tatcgtcaat gcgctatccg aactggataa
    11941 cgaacaaact atcctgatgc caggcggaac gtttcgtaaa aaatcggcct catttcacgg
    12001 gcagctggca gagaatgcct tcgagcattt caccttcgac aaattattca tgggtactga
    12061 cggcatcgat ctcaatgcgg gcgtaaccac cttcaacgag gtctataccg tcagtaaggc
    12121 aatgtgcaat gccgcgcgcg aagtgatttt gatggcggac tcatcaaagt ttggccgtaa
    12181 aagccccaac gtagtttgca gtcttgaaag cgtcgataag ctgattaccg acgcagatat
    12241 cgatccggcg tttcgtcagg cgctggaaga gaaagggatc gatgtgatca taaccggaga
    12301 gagcaatgag tgaagcacta ctgaacgcgg gacgtcagac gttaatgctg gaattgcagg
    12361 aagcaagccg tttaccggaa cgtctgggcg atgattttgt tcgcgccgcc aatatcatcc
    12421 tgcactgcga aggcaaagtg gtggtttcgg gaattggcaa atcgggccac attggtaaga
    12481 aaatcgccgc aacgcttgcc agtaccggca caccggcttt ttttgtccat ccggcagaag
    12541 cgctgcacgg cgatctgggg atgatcgaaa gccgcgatgt gatgctgttt atctcttact
    12601 ccggtggggc gaaggaactg gatctgatta ttccgcgtct ggaagacaaa tctatcgcgc
    12661 tgctggcgat gaccggcaaa ccgacgtcac cgctgggcct ggcggcgaaa gcggtgctgg
    12721 atatctccgt agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc agcaccgtca
    12781 ataccctgat gatgggtgac gcgctggcga tggcagtcat gcaggcgcgc ggatttaatg
    12841 aagaagattt tgcccgctcc caccctgctg gagcactggg cgctcgcttg ctgaataaag
    12901 tgcatcatct gatgcgccgt gacgatgcca tcccacaggt ggcgttaacc gccagcgtga
    12961 tggatgcgat gctggaactc agccgcaccg gtctggggct ggtggcggta tgtgacgctc
    13021 aacaacaggt acaaggcgtc tttaccgacg gcgatttacg tcgctggctg gttggcggcg
    13081 gcgcactcac cacgccagtc aatgaagcga tgacgacagg cggcaccaca ttacaggcac
    13141 aaagtcgcgc catcgacgcc aaagagatcc tgatgaagcg caaaatcact gccgcaccgg
    13201 tggtggatga aaacggcaaa ctcgtcggcg caattaacct gcaggatttc tatcaggccg
    13261 ggattattta atccttcaat cccagacgtt tcgccagccg atgcaggttg gcgacgtcgg
    13321 tttccagcat tcgcgcgcag gctgcccagt tgtgatgatt ttgtgccagt gcctgacgaa
    13381 tagtttcacg ctggaacgct tctgtcgctt cacgcaggtt ttgcttaaca acgggcaccg
    13441 ccgccgcttc tggcggcggt aacgtcacct caggaaaagc aaaatgttgc gcctcaagaa
    13501 tcacttcatc gccgttgcgg gtggctctcg ccagcactac cgcccgatga atagcatgtt
    13561 ccagttcgcg cacgttcccc ggaaaacgat agtgttgcag taaatttcgc gctccggcac
    13621 ttaataccac gcgggagagc ccctgccgca aacgacactg ctcgcagaaa taccccgcca
    13681 gcagaatgac atcatcgccc cgctcacgca gcggcggcac cgaaagtgga aacacgctca
    13741 ggcggtggaa caagtcagcg cgaaatcgcc ctgccagcac ctcttcgcgt aaatcgcggt
    13801 tagtcgccgc cagcacgcgc acatcgaccc gcaaactacg gtcatcgcca acgcgctgaa
    13861 tatcgccata ctgcaacacc ctcagcagct tggcctgcaa tgccaacgac aactcgccga
    13921 tctcatccag aaacagcgtg ccgttatccg ccatttcaaa cttcccactg cggttactga
    13981 ttgcgccagt aaacgctcct ttcacatgcc cgaacaactc actttccgcc acactttccg
    14041 gcagtgcggc acagttgaga tagaccagcg gattcaccgc ccgtggcgag gcctcatgaa
    14101 tcgctttcgc caccagctcc ttaccggttc ccgtctcacc gctgatcagg acgttgagat
    14161 cggacgccgc cacaatctca atctcttttt tcagttgcgt catgcctggc gacagaccga
    14221 tcatctgcgt ctgtttcacc gcttcaaatc gagcggcatc gcccggcagc atattctggc
    14281 tttccagttg ctcaatcagc aacgcattgc ttaacgctcc cgccgccagc gcggcaatca
    14341 ggcgtaactc ttcgtcgctg aaaacatcga actgatcggg ctgcatcccg tcgagcgtca
    14401 atgcgccgat caggttttgc ccggcaaaca gcggcagacc aacgcaggcg tgaaccttca
    14461 gactctcctg ccctggaatc aaaccgtcat agggatcggg caattcgctg tctgcgggaa
    14521 agcgcaccac atcacccgcg cgggcaatcg cttccagccg tggatgacct tccagcgcaa
    14581 agcgtctacc gagtacatcc ttcgccagcc cgtcgatggc aagcggaata aactgccgcg
    14641 agtcgtaacg tagcaacgca gacgcatcgc actccagcac ctgacgcagc gtggtgatca
    14701 ggcgctgaaa acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg atattcgcca
    14761 gcacatcaac ggaaaaactc atctttgcct cactgtcaat ttgactatag atattgtcat
    14821 atcgaccatt tgattgatag tcattttgac tactcactaa tgagcataat tttatttata
    14881 gagtaaaaac aattagataa aaaactggca cgcaatctgc aattagcaag acatcttttt
    14941 agaacacgct gaataaattg aggttgctat gtctattgtg gtgaaaaata acattcattg
    15001 ggtgggtcaa cgtgactggg aagtgcgtga ttttcacggc acggaatata aaacgctgcg
    15061 cggcagcagc tacaacagct acctcatccg cgaagaaaaa aacgtgctga tcgacaccgt
    15121 cgaccataaa ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg atctggcgga
    15181 tatcgattac atcgtgatta accatgcaga agaggaccac gccggggcgc tgaccgaact
    15241 gatggcacaa attcccgata cgccgatcta ctgtacagcc aacgctatcg actcgataaa
    15301 tggtcatcac catcatccgg agtggaattt taatgtggtg aaaactggcg acacgctgga
    15361 tatcggcaac ggcaaacagc tcatttttgt cgaaacacca atgctgcact ggccggacag
    15421 catgatgact tacctgacag gcgacgcggt gctgttcagt aacgatgctt tcggtcagca
    15481 ctactgcgac gagcatctgt tcaacgatga agtggatcag acggagcttt tcgagcagtg
    15541 ccagcgttac tacgccaata tcctgacgcc gttcagccgc ctggtaacgc cgaaaattac
    15601 cgagatcctg ggctttaact taccagtcga tatgatagca acctcccacg gcgtggtctg
    15661 gcgcgataac ccgacgcaaa ttgtcgagct gtacctgaag tgggccgctg attatcagga
    15721 agacagaatc accattttct acgacaccat gtcgaataac acccgcatga tggctgacgc
    15781 tatcgcccag gggattgcgg aaaccgaccc acgcgtggcg gtgaaaattt tcaacgtcgc
    15841 ccgaagcgat aaaaacgaaa tcctgaccaa tgtcttccgc tcaaaaggcg tgctggtcgg
    15901 cacttcgacg atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg aggagatgac
    15961 cggattacgc ttccgtaaca aacgcgccag tgctttcggc tctcacggct ggagcggcgg
    16021 tgcggtggat cgtctttcca cgcgcctgca ggatgcgggt ttcgaaatgt cgcttagcct
    16081 gaaagcgaaa tggcgaccag accaggacgc tctggagtta tgccgtgaac acggtcgcga
    16141 aatcgcccgt cagtgggcgc tcgcgccgct gccgcagagc acggtgaata cggtagttaa
    16201 agaagaaacc tctgccacca cgacggctga cctcggccca cggatgcagt gcagcgtctg
    16261 ccagtggatt tacgatccgg caaaaggcga gccaatgcag gacgttgcgc caggaacgcc
    16321 gtggagtgaa gtcccggata acttcctctg cccggaatgc tccctcggca aagacgtctt
    16381 tgacgaactg gcatcggagg caaaatgagc aacggcattg tgatcattgg ttcgggcttt
    16441 gccgcccgcc aactggtgaa aaatattcgc aaacaggacg ccactattcc attaaccctg
    16501 attgccgccg acagcatgga tgagtacaac aaaccggatc tcagccatgt tatcagccag
    16561 gggcaacgtg ccgatgacct tacccgccag acggcgggtg aatttgccga gcagtttaat
    16621 ctgcgcctgt ttccgcacac ctgggtgatg aatatcgatg ccgaagcccg tgtggtgaaa
    16681 agccagaata atcagtggca atacgacaag ctggtgctgg caactggtgc cagcgccttt
    16741 gtcccgcctg tgcctgggcg tgagttaatg ctgacgttaa atagtcagca agagtatcgc
    16801 gcctgtgaaa cgcaactgcg ggatgcccga cgcgtgttga ttgttggcgg tggtttgatt
    16861 ggtagcgaac tggcgatgga tttttgtcgt gcaggcaaag cggtcacgct aatcgacaac
    16921 gctgccagta ttctggcgtc gttaatgcca ccagaagtaa gcagccgctt gcagcatcgg
    16981 ttgacggaga tgggcgttca tctgctgttg aaatctcagt tgcaggggct ggaaaaaacg
    17041 gattctggca ttctggcaac gctggaccgc cagcgtagca tcgaagtgga tgcggtaatt
    17101 gccgccaccg gactgcgccc ggaaaccgcc ctggcacgac gcgccgggct gacgattaat
    17161 cgcggcgttt gcgtcgatag ttatctgcaa accagtaatg ccgatattta cgcgctgggc
    17221 gattgcgcgg aaattaacgg tcaggtactg ccgttcctgc agccgattca acttagcgcg
    17281 atggtgctgg caaaaaatct tctcggcaat aacacgccgc tgaaactacc ggcgatgctg
    17341 gtgaaaatca aaacgccgga attaccgctg catctggcag gcgaaaccca gcgtcaggat
    17401 ttacgctggc agattaatac cgaacgccag ggaatggtgg cgcgcggtgt tgacgatgca
    17461 gaccaacttc gcgcctttgt ggtcagtgag gatcggatga aagaggcatt tggattgttg
    17521 aaaacattgc cggtgtaggc gggctactgt gcccaaaatg tcggatgcga cgctgacgcg
    17581 ccttatccga cctacgggga gcgcatgtgt tggtcacata atgcgttcat gtcgcatccg
    17641 cctactgtgc ccaaaatgtc ggatgcgacg ctggcgcgtc ttatccgacc tacggggacg
    17701 catgtgtagg ccggataagg cgtttacgcc gcatccggca atggtgtcca aatgcaacac
    17761 gatttatccg ttctggactt cacccgctaa ccaacgcgcc gcagcaataa ccccctgccc
    17821 cagagacaaa ccgccatcac ccgccggtaa actctgtgga aagagcaatg tgaaatcagc
    17881 gagataatgc gccagacgtg cacgcagcaa acggttatga ataaccccgc cgctaaatac
    17941 cagcgtagtg ataccacgca tcgtggcctg ctcacgcatc aacgcggcaa aaccctgcgc
    18001 cagcgcatca tgaaacgccc acgcgcgttg attaaccggt gcctgccagt tcagccactg
    18061 ctgccagaaa gtggtgagat ccagttgatt gtccaccagc ggcattgtca ccggatgcgt
    18121 cactccgtgg catgaggctg cgagcgcctc cagagcacaa gccgcttcac cttcataact
    18181 taacgtggct ggcgcacagc ccagtgccgc cgccactgca tcgaacaccc gcccacacga
    18241 tgacgccagc ggcgcgttaa ttccacgctc aatggcccgc gccagaacgc tccagttttg
    18301 ctgttgtaca cttgccgttt cagggtaatt ctgccactcc ggcacaaagc gcaggcactg
    18361 cgccagcagg ttacgccacg gctgcttcgc cgccaaatcg ccacccggaa gcgccactgc
    18421 aggcaagccg cccaggtgct cacattcgcg atagttcacc cgcaggcact cgccgcccca
    18481 caaagcgccg ttctccccca taccgatacc gtcgagcgtc aaagcaatga catcaccgcc
    18541 ctccagtggc cagtgatgtt ctgccagaca cgccgccgcg tgggcatgat gatgcagcac
    18601 cgtttgcgtc ggcagattca tttcgcgcgc ccactggctg gagacatagc ccggatgtgc
    18661 gtcatgcaca acgtattgcg gggtgaaatc gtagatgttt tgcatcaggc gtaacgcttc
    18721 gcgccactgc atctggatgc catcgtcact taaatcgccc agatgctggc tcaacaccgc
    18781 ttgttcaccg cgcaccaggc agaaggtatt tttcagatcc gcgccgagac acagcacagg
    18841 cggaacattt ttaaagcccg gaggcaaagc cagcgcatcc ggcacatacc cccgcgaacg
    18901 gcgtagcatt tcgccgcttt cgcgcaccac cgaatcatcc atccgctgca cgatgtcgcg
    18961 gttatgtatc aagaatccgt cggcaatgcc ctgcaaatct gccagcgcct gttcgttgct
    19021 gatagccggt ggtttaccgc tcaggttgcc ggaggtcatc accagcgggc attgcagttc
    19081 ctgtaacagc aaatgctgga gcgggttcgc aggcaacatt accccgactt cgttaaggtc
    19141 aggggcgata tcatcacaaa gctcaggtac gtatttttta tccaccagca caatcggcgc
    19201 ggcgggcgtg gtaagcaact ggcgcgcagc gtctggtaaa ccgtcagcca ctggcaacat
    19261 gaccgccagc ggtttcgccg ggcgatgttt gcgcgcccgc agtgtcgcca ccgcgttact
    19321 gttacgtgca tcgcaggcaa gatgaaatcc gccaatccct ttgatggcga caatgttgcc
    19381 catttttaac tgtgcgatag ccgcctgtaa tgccgcctct tgttctgcat gttcaccatg
    19441 acttacccat tcaagatgcg ggccacactc cgggcaggcc accggctggg cgtggaagcg
    19501 acgatcgagc gggtcgcggt actctttatc acaggctgga cacagcggaa acgccgccat
    19561 cacggtaaac gggcgatcgt aaggcatggc gcgaataatg gtgaaacgcg gaccgcagtg
    19621 ggtacagttg ataaacggat aacgccagcg tcgctcgcca ggggtattca tttcggcaag
    19681 gcaggcaggg caagtggcgg catcgggaac aatttgcgta ttcatggcgc cgcccgcgct
    19741 ctggcggata gtgaactcgg tgggcagttg tgaccagata aacggctcac gctcgacgct
    19801 atcaatacgc gccagcggcg ggcagtgctg atgcaattga acaagaaacg tttccgggtc
    19861 ttccagcagc cggacttcta cgccatcgcc gtcattacag acatcgccgt gaagatttaa
    19921 ttgctgtgcc agctgccaga caaacggacg aaaaccgacg ccctgcactt tgccacgaat
    19981 acgcagttgg acaccgcaag atgtgttttt tgccattgag ttattcccgc catcatgaat
    20041 tgcgtaaccc gtccagccgg acacgacagc gtcgcatccg gtagtcacag gtcggcgata
    20101 ctgcccgctt cgtattctac gaatatttcc gggaattcct ttgatgccag aacagttctg
    20161 taagattttt agaacatcag cgccgtgcgg cgacgttttt ctgcgctcag ttgttcaagt
    20221 ttattacgat cgacacaaat cagcgcatga gtcgggcaag ccgccataca cgccggaccg
    20281 tcttcacgat ggttgcacag gtcgcattta ttggcttcgg ctttgtcagc ccgtacattc
    20341 agccctgcgc cgctgttgcg aatcaccgga cgtaccacca cttccatcgc gccatacggg
    20401 caagccacaa cgcaggtttt gcaaccaatg caacgttcct gcatcacatg aacaaaccct
    20461 ttatcacggc tgatagcacc attcgggcag acgttagcgc acggtgcatc ttcacactga
    20521 cggcaaactg tcgccgtgga aatgttcaca cctttaatga catggatacg cggtaaaaat
    20581 gtttccgggg tcagcgatgc acagtcctga ttttcctgat gagaaaccac gcacgccact
    20641 tcacaggtac ggcaaccaat acatttactc gcgtcagcaa tgatgaaacg gttcatcaaa
    20701 ttctccagca atgacagtta atgcgccgat acattcacaa atcatgccag tttttaattc
    20761 actgttattt aaggaaatta atttctgtaa tgcaggaaaa aaagatgtca tcgacactag
    20821 tgacgatgac atgtgatgac aatgtttatc gcgaaagagc aatgagtgag tcgcggcgga
    20881 tcagttttcc gctgaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca
    20941 gccgtccaat aatttcctga atcatctctg ttaccggaat ttttacgctg gagagcgccg
    21001 gaacggtgta gggggcaata gcgatatcat cgaatcccat aactgacacc tgctctggca
    21061 ccgctacgcc gcgctcgtgt aacgctttca tcgcacctat cgccatatcg tcgttactgg
    21121 caactaacgc gctaaattta gccccacgtt cgagcaacat ttctacccct tcggccccgc
    21181 tggcaggcgt ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg
    21241 acagcgcgtc tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg
    21301 taaggaaagc aatctcctga tgtccggcgt ttatcaactc tgccacggcg ttaaagctgg
    21361 tctgtttatg atcgcaccag acgctatggc tgctgttttt gcgcaggcgg cgattgagca
    21421 ccattatcgg ctgactgtgc gcgtcaatga tgtcatcgat ctcatccacg cttaaaaaac
    21481 gcgggtaaat cataatcgcg tcgcagcgca aatccagcag atactgaatc gcctggcgct
    21541 cttcttcggc gctgtgttta ccatctgcca atagtaactg tcgccctttc tcttccgcca
    21601 ttcgcgcggc atgaaacagt aattcactaa aataaatgcc gtggtaaagc gtgttggtca
    21661 ctaccagccc cagcgtctga gtactcttcg ccgacagatt acgcgccagc aagtttggac
    21721 ggtaaccgct ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc
    21781 cattacctga aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt
    21841 ccagcatcgt cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg
    21901 tttgccgatt gtccttgcgc aatcggcggg aaaaatattc aggtgaccgg tttcacaaat
    21961 ataaaaaatg aacaattaac tctcttgctt atttggtgac aactattcat gattttgtga
    22021 aaccggtttc ttaattccgt ttcagcatcg gcatttttcc gtcacgtcga ctgataacaa
    22081 ccacatctac cctactgata acaggataaa atccgatggc caaaaattat gcggcgctgg
    22141 cacgctcggt gatagcggca ctgggcggcg ttgataacat ctcggcagtc acgcactgta
    22201 tgacgcgttt gcgctttgtt atcaaagatg atgcgcttat cgacagcccg acgttaaaaa
    22261 ccatccccgg cgtgctcggc gtggtacgca gtgacaacca gtgtcaggtg attatcggca
    22321 ataccgtttc acaagccttt caggaaatcg tcagcctgct gccgggagat atgcagcccg
    22381 cacagcccgt aggtaaaccc aaactcacgc tacgtcgcat tggtgcgggg atcctcgatg
    22441 cgctgatcgg caccatgtca ccgctgatcc cggcgattat cggcggatcg atggtcaaac
    22501 tgctggcgat gatcctcgag atgagcggcg tgctgacaaa aggatcgccg accttaacca
    22561 ttctgaatgt gattggtgac ggtgctttct tcttcctgcc gctaatggtc gcggcatctg
    22621 ccgccatcaa atttaaaacc aatatgtcgc tggcgattgc catctcgggt gtgctggtac
    22681 atccgagttt tattgaactg atggcgaaag cggcccaggg ggaacatgtc gaatttgccc
    22741 tgatcccggt caccgcggtg aaatacacct acacggtgat cccggcgctg gtgatgacct
    22801 ggtgcctgtc atatatcgaa cgctgggtgg atcgcattac acccgcggtg accaaaaact
    22861 tcctcaagcc aatgctgatt gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc
    22921 cgattggtat ctggatcggt agcgccattt cggcgctggt ttacaccatt catggttatc
    22981 tgggctggct ttcagtcgcc attatgggcg cgctgtggcc tctgctggta atgaccggga
    23041 tgcaccgcgt ctttacgcca accatcattc agaccattgc cgaaaccggc aaagaaggga
    23101 tggtcatgcc gtcagagatc ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg
    23161 cgtggaaaac gaaaaacccg gaactgcgcc agactgcgct ggctgcggcg gcatcagcca
    23221 ttatggcggg gatttccgaa ccggcgttat acggcgtggc gatccgcctg aaacgtccgc
    23281 ttatcgccag tcttatcagc ggttttattt gcggcgcggt tgccggtatg gcggggcttg
    23341 ccagccactc aatggcagcg ccggggctat ttaccagcgt gcagttcttc gatccggcga
    23401 atccaatgag catcgtctgg gtgttcgcgg tcatggcgct ggcggtggtg ctgtcgttta
    23461 tcctcacact gttgctcggc tttgaggata ttcctgttga ggaagcggct gccgaggcgc
    23521 gaaagcatca gagcgtacaa ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa
    23581 aatgtcagta tttccagaag gttttttatg gggcggcgcg cttgccgcca accagtctga
    23641 aggtgcgttc cgtgaaggtg gcaaaggact gacaacggtc gatatgatcc cacacggcga
    23701 gcatcgaatg gcggtgaaac tggggctgga aaaacgtttt cagttgcgcg atgacgagtt
    23761 ttatcccagc catgaggcga cgggttttta tcatcgttat aaagaagata tcgccctgat
    23821 ggcagagatg ggattcaagg ttttccgtac ctcaattgcc tggagccgtc tctttccgca
    23881 gggcgatgaa atcacgccca atcagcaggg cattgctttt tatcgttctg tctttgaaga
    23941 gtgtaaaaag tacggtatcg aaccgctggt cacgttgtgc cacttcgatg tgccgatgca
    24001 tctggtcacc gaatatggct cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta
    24061 cgcccggacc tgctttgaag catttgatgg tctggtgaaa tactggctaa ccttcaatga
    24121 aatcaacatt atgttgcata gcccgttctc cggcgcgggt ctggtgtttg aagaaggtga
    24181 aaatcaggat caggtgaaat atcaggccgc gcatcaccag ctggttgcca gtgcgctagc
    24241 caccaaaatc gcccatgagg ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg
    24301 caacttctac ccttacagtt gcaagccgga agatgtctgg gcggcgctgg agaaagatcg
    24361 ggaaaacctg ttttttatcg atgtgcaggc gcggggcacg tatccggctt actctgcccg
    24421 cgtattccgc gaaaaagggg taaccatcga caaagcaccg ggcgatgatg aaatcctgaa
    24481 aaacaccgtc gattttgtct ctttcagcta ttacgcctcg cgctgcgcct cggcggagat
    24541 gaacgccaac aacagcagtg cggcgaacgt ggtgaaatcg ctgcgtaacc cgtatctgca
    24601 agtgagcgac tggggctggg gaattgatcc actcggtctg cgtatcacca tgaacatgat
    24661 gtacgaccgc tatcagaagc cgctgtttct ggtggaaaac ggcctcggcg caaaagatga
    24721 acttgctgcc aatggcgaga ttaacgatga ctatcgcatc agctatttac gcgaacatat
    24781 ccgcgcaatg ggcgaagcga ttgcagacgg cattccgctg atgggctaca ccacctgggg
    24841 ctgtattgat ttagtttccg cctctacggg tgaaatgagc aaacgctacg gttttgtctt
    24901 tgttgaccgt gacgacgcag gcaacggcac gctgacgcgc acacgtaaga aatcgttctg
    24961 gtggtataaa aaagtgattg ccagtaatgg tgaagattta gagtaagtaa ccgtgccgga
    25021 tgcggcgtga acgccttatc cggcctacat agttcgtgcg acatgtaggc ccgataagcg
    25081 cagcgcatcg ggcaatttag catttggcat cagagctggt tcaccccatc aagaacatcc
    25141 ctgtcctgat tccttattga aaaactactc ttcttccacc gctaactgcg cgaagccgcc
    25201 gttcccttcc cagccttcca gtcgttgata aacggtttct accgcatctt taatcggctg
    25261 ggtcatcggg tagtaaaagc cgacgatatc cggctggatg ccgaggaaaa tcacttcgcc
    25321 aatatcttct ttcaactgat cgataaggta gttcaacggc atgttatggg tagtcatcat
    25381 aaacatctcg gcgatatcat ccgggtcgat gatgcggatt tcgccggggt ttagccccat
    25441 atcagtagcg tcaacaatca gtaaccgcgt agggcgcagt tcacggatgg cgacgatgtc
    25501 gttttccggt gcgctaccgc cgtcaatcac cacccagtta cctttcggcg cggcggcgca
    25561 cttttccgcc agcagcggac ctgcgccatc atcgcccatc atgctattgc caacacagag
    25621 taaaacgtca gtcacgcagt ctcctcacca tcaaatagat ggcgttttcc tgatgaatat
    25681 catgcagcat acttaacagc attgtactcc attcctgctg ctgcgaagtt tgcgctgccc
    25741 gggctttatc gaatgcattg gccagcatcg gcacatggtt aatgtcgatg acgatctcac
    25801 catatttcgg cacaccttcc attttgcggc gggcttcgct gcccgcctcc agagtggcaa
    25861 tccatgccag atattcatcc cacgggcagg tgagcgccgc ttccaggcaa tcgataaccc
    25921 caaggtggtg accaatcgcc aggctgtaat agaccacctg ctgcgcctcg gcgggcgtgg
    25981 catcgttctc atcaataaat ttacggctca gttgactgaa caccaccttt tcactcatcg
    26041 gatacgcgcc tcttctacaa catgattcag atggctgaca atctcgttca gacgcggatc
    26101 gttttccgct tccagccagc gtgcaacctg ttcttcgccc tgccctaact gtgtaaggta
    26161 atcatcggca atctgacgac cgtaacgata acccgccagt cgacgtgctt cgcgatccac
    26221 tttcacacgc agcggctgca ccatattgcc atgcagaatt tctgccggtt gttcatcctg
    26281 ctcacccggc ccacgggcgt gaattttctg ctccagcagg ccgagcgcca ttgcaaagcc
    26341 gtacagcgtg gcggcaggcg ttggcgggca gccagggata taaacatcca ccgggacgat
    26401 tttatccgta ccgccccaca cgcagtagag atcgtggaag atcccgccgc tgttaccgca
    26461 ggcaccgtag gagatacaaa ttttcgggtc cggcgcggac tgccacgcac gcagcgcagg
    26521 ggatcgcatt gcacgggtga ccgcgccggt aaacagtaaa atatccgcat gacgcggtga
    26581 aggaacgact ttaatgccga agcgttctgc atcgaacagc ggcgacaaag tggcgaaaat
    26641 ttcgatttcg cagccgttgc agccgccgca gtccacgcgg taaacatagg cagaacgttt
    26701 gatttttttc agtaacgacg ccttcatgct ggcgatggat tcatccaccg tcatcgggac
    26761 cggaatgccg ttggcgtcac gggggcctaa taaattgctc atcagatggc ctctttcata
    26821 tggcgagtca gttcaatacg gtcggacggc accaggcatt tctggcgctt acattccggg
    26881 caagtctcaa agctttcgcg gtggttttcc gcgcggctgt cgccgttgtg cttaagcagc
    26941 gcaatggcgt agtcgatctc tttctggacg gcgaaaggac gattgcagac gcgacagttg
    27001 cacagcgcga agcgggactg ttgcaggaag tcttctttct tccacaccgc cagttcgtac
    27061 tcttgcgaca gcttaatcgc cgccgtcggg cagacttctt cgcagcgtcc gcagaagatg
    27121 caacgcccga gattaaactc ccaggccagc tctcctgtgg cgaggtcagt ttcaaccgtt
    27181 aaggcgtttg acgggcaggc attgacgcag gccgcgcagc cgatgcactg ctgcgggttc
    27241 tgctctggct taccacggaa gtttttatca accgcaatcg gctccagcgg ataagacgag
    27301 gtcgccgtgc cggttttgat gacttttttg ataaaggtaa acatggcgat tccttatttc
    27361 agcggcgagt ttttacgctc aatgctgtaa cgctcgagtt ctttgtacgg caccactttg
    27421 ctcttcttct tacgcacatc gaccacggtc atgcggtcgg tacaggagta gcaagggtcg
    27481 aggctaccga taatcagcgg cgcatcggaa acggtattgc cacgcagcat gtagcgcagg
    27541 gtcggccagt tcgcgtaagt cgcggcacgg cagcgccagc ggtacagctt ctggttgtcg
    27601 ccggtcatgc tccagtggat atcatcgccg cgcggcgctt cggcaaagcc cagcgcaaag
    27661 cggtgcggaa tgtaggtaaa gccttccacc atcagtgggc cacccggcag gttatccaga
    27721 ccgtagtcga tcatgttcag cgcggtatag acttcgttga tacgcacttt cagacgggaa
    27781 ataacgtcgc agccctgctc gctgtggact tccattggca gcaggccata accaacaaac
    27841 gggtgatcgg cgcgagtatc gcgagcgtga ccgctggcac ggaccatcgg gccgacgtta
    27901 ctgaagtcgc gagcgatttc cgggtccaga cgaccaatgc cgacagtgcg ttgttccatg
    27961 ttcggtgtgc tcaacagcac atccaccagc tcctgcactt cacgacgcat ctgttgcgcc
    28021 agctggcggg tctggatcat gtcatctttc agcagatcgc gacgaatccc gccgatcagg
    28081 ttcaggccgt aggttttacg cgcaccggta aggatctctg ccattttcat ggaggtttca
    28141 cgcacgcgga agaactgcat aaagccggag tcaaagccgg taaagtggca ggcaagaccg
    28201 aggttgagca gatgcgagtg caggcgttcc acctccagca gaatggcgcg gatcatctgc
    28261 gcacgttctg gcacctgaat acccatcgcg ttttccaccg acgtggtgta ggcggtgctg
    28321 tgagcaaagc cgcagatccc gcacacacgg tcagagagga aggtcacttc gttgtaaccc
    28381 atacgggttt ccgccagttt ttccatgccg cgatgaacgt agaacagacg gtagtcggcg
    28441 tcgataatgt tttcgccatc gacgaacaga cggaagtgac ccggttcgtc agaagtgacg
    28501 tgcagcggac caatcggcac gacgttgttt ttcttgtcgc ccagttcgtt gatgaactcg
    28561 taggtttcag catcggtggt cggtgccgga cgttgacgat aatccatgct gtctttacgc
    28621 agcggataaa gttcatccgg ccagtcatcc ggcagcacca gacggcgttc atccggcagc
    28681 cctaccggaa tcaaaccgta catatcgcgc acttcgcgct cgccccacac cgccgccgga
    28741 acgcgcggcg tcacggacgg atactccggt ttgttggcgt caacttcgac gcgaaccgta
    28801 atccaacact tagtgccctt ctccatcgac agcacgtagt aaacggcgta atgaccattc
    28861 agtttgcgtt cgtcgttacc aaacagcacc gacagccagc caccctgttt gtagtagaga
    28921 aactccacca cttccggcag atagttcacc ttcacggtca cggtcagctg atctttggtc
    28981 tgccaggcgt ggtccagcac gacgcccgga aatgcctcat tcagcgcggc gagataatgt
    29041 tgacctaatt tttcttcaga catgctcaaa ctctctttaa tcacgccgcc agcaaggaga
    29101 cgaacgctaa aaatgcaaag ccaaacccag cccaggtaat gcgcggagta atatcaagac
    29161 gcagacgggc catgctgttt tcgaacagcg cgataaccag gacgccgacc accagtttga
    29221 cgatggcaat caccagcgcc agcagcagtc caccagcggt gaaggtttcc atttgtcccc
    29281 acggaataaa caccccgacg aacatctgca gcaccaccag ctgtttcagg ctgatacccc
    29341 atttcatgac gccaaagccg ctgccgctat actcagagag cggaccttcc tgcaactcct
    29401 gctcggcttc cgccaggtcg aacggcagtt tgcccatttc gataaaagtg gcgaacgcac
    29461 aggcacaaag cgccagcacc agcgggatgc tctggctcag cggccagtga taaacggtgt
    29521 cggtgatgtt gctgatgttg gtggaaccgg caacctgtgc ggcaacccac agaccaagca
    29581 gcaacatcgg ttcgaccagc acgccaagca tcgcttcacg gctcgcgccg atagcggtaa
    29641 acgggctacc ggtatccaga ccagaaatgg caaagaagaa acgcgcgatg gcaaagagat
    29701 acagtaaggt gatcaaatca cccagttgcg gcagcggaga accaacggtc accaccggca
    29761 gcgcagtagc gatagtcagc atgacgccca ccatcacata cggcgtcagg cggaacaccc
    29821 agccggaggc atccgggcca acgctctggc gacccagcag tttgataatg tcgcgatact
    29881 cctgcaacac gcccggcccg cgacggttat gcaagcgggc gcgcgccacg cgggttatac
    29941 cggagagcag cggcgcaacg gcaaatagca ccagcgcctg aattaacgga tataaaacac
    30001 tcattctcag gctcctcgtg aaacaataat caccaccagc accgccagtt caaccagcgc
    30061 catccggcgg aacagcaacg cactcccctc gcactgccag cccggcacca gagacaccgg
    30121 attcagccat ttgcgtagtt tcagcaccgg cgcaaacgcc tgtttcaccg gcatggcaaa
    30181 accgtgagcg gtaatcacca ttgatttttc gtggtcgtag ccgcacaccc aggccgcgcc
    30241 gcgggaacgc gatggcaaac gatcgccttt gcaaatcgcc ataatgatga atggcagcag
    30301 cgggcaggca atcagcagca acgtgatcat tggttgagaa acggtggtgt tagcaggctc
    30361 cagaggcaga ggtactgcag cagagagcat cggcagcagc cacggcgcag caacaccgcc
    30421 aattacgcag caaatcgcca gtgccactac gcttacgctc atcaggagcg gtgcacaggt
    30481 ggcgttttcg gcttctttgg tacgcggcgc gccgaggaac gtgacgccat agactttcgc
    30541 catacacatc accgccagcg caccggtaat tgccagcccc acagcgagca gcggccccag
    30601 cagacgggca acaaacgcgc cactattgct cagtttgaaa aaggattgat agataaccca
    30661 ttccccggca aacccgttca gcggcggcag cgcagccatt gccatcaacc cgactaacat
    30721 ggcgatggag ataaccggca ttttcttgcc aataccaccg agtttttcga tatcgcgatg
    30781 accggtacgg aaccagacgc tccccgcccc gaggaacagt acgcttttga acaggctatg
    30841 gttaagcaga tggtacagac caccgaccag accaagggca atcagcgccg gttgttcgag
    30901 cgcgataccc gttacgccag cgcccagccc cagcaggatg atgccgatat tttccagggt
    30961 gtggtaagcc agcaggcgct ggatgttgtg ctccatcagc gcatacagac caccgacaaa
    31021 cgcggtgatc atgccgagca ccagcagcgc gatcccccac cacagcggtg cattaccgcc
    31081 cagcagtgac agggttaaaa tgcccagcag gccaattttc atgactaccg tagaaaacaa
    31141 cgcggcagct ggcgcagagg cgttagcatg tgcctgcggc acccagccgt gcagcggaat
    31201 aatcccggcc agcaggccaa agccaatcac accgagcagc cagatatcgg aaccgagcgg
    31261 cagctgttgc atacgcatat ccagcaggcg cagatccagc gtgccataac gctgccacag
    31321 cagccagcaa gcaatcacca gcagcagagt gccaagacgc cccagcgcaa accacagttt
    31381 gccctctttg ctgttgctgg tgaggaacac cgcacacaag gccatgactt cggccattac
    31441 cacgaacatg ccgaggttgc tggcaatgac ggcgcagacg gcggcagcca tcaacatatt
    31501 gatctgcaag ccgttgcact tcacctgcgc gtggcgatgc cagtcaatgt tgtagaggct
    31561 gacaaacaga ccgcacagac cgagcgtaat cagccaaatc gcgttaagcg gagagatttg
    31621 cacatcgtag cttaccagcg acagcgcacc gctcacgcca accgcgccag tcagtacagt
    31681 gaagcccgcg gctgccgtat acagactacc aaccgcgccg ccaattccgg ctatccagcc
    31741 gcttagcgcc ttttgaaaag aaaagagaaa tgccagaaca gcggcggcga caaaccacgc
    31801 cacgccgcta ttgatcaggg aaattgcgct catttagcct ctccactttg agcctgctga
    31861 aacaaggtga gatcgccaaa gtccgtgtta aaggtcagct cacgcttacg tttgctgacg
    31921 cgggcgatat cggtgttatc caccagatgc agggctttag tcgggcacat ccgcacgcag
    31981 gccggacctt gttcatcaaa gctacaaagg tcacatttga cggcgatcgc gcgaatacct
    32041 ggcacccagt caagcaatgt gctgacacgc gccggagcag gcggtgccgg tggcgctttc
    32101 ggggtattgg cgtttgccgg aatatccagc ggacggctgc cggaaaattc aattgcgcca
    32161 aacgggcagg cgatgccgca cagtttgcag cttacgcaca ggctttcatt caactgcacg
    32221 gccccatcaa cgcgggtgat ggcgttaacc gggcagacca ccgcgcaggg tgcatcttca
    32281 cagtggtgac agagctgcgg cgcagattct ttttcattca gcatcactct caggcgcggc
    32341 attgattgca ggccgtgctg gcgatgcgtc tctgaacagg cggcctcaca agtgtggcag
    32401 ccgatacaga gcgtggagtc agcaattaca aaacgattca ccaggcattc ctcaggtgat
    32461 tgtcattttt gacgaaaaca tgccgatgaa atgtcatttt cgacactcat cgacacgccc
    32521 atccccaaac aggcgtaacg cctgcaaaac gggcaaagcc tcagctcatg ctgccgggct
    32581 ttgtcccttt accagttggc ttaaattcac cggcacattg ttttcaatgg cggtgtataa
    32641 gctgtcgtaa acgccagcga ttttttcgag atagtgttcc agaacctgtt cgcgatcgcg
    32701 gatgttgacc ttgccatcaa tcatgccgtc aatgctcagt tgcgcctgcg caatccgctg
    32761 tttgtcttcg ccatcttttg tttcgacgta atactcgatg gtgtggctgt taaagccatc
    32821 cgccagggtg acgtaaatac gaaaatgttc aaaaaggacg aaacagagtt ctttgtccgg
    32881 cgcgcagctc atggcgtgat gcaggcgcgc tttcgataac gtgatcccct gaaccagcga
    32941 attgcagtag atgtgccact ggtcctgtag gcgacgatgc cgctgtgcga tgtaatcggc
    33001 tttctcgctt atttcccaaa tagtcattgt caggttaccc gtttaacaga gatgccagct
    33061 ttaagcattt tttgtgccaa cttttaattt attgttatta agcgattttt aagattttgg
    33121 tgaatttcgt cgccgtgtcg acgtgtcatt tcgacatcat cgacattatt cactgcaggg
    33181 ataatcaaca ctggcacaat tattgcttgt agctggcaat agttaatggg aggcgatatg
    33241 cacgaaataa ccctctgcca acgggcactg gaattgatcg aacagcaggc cgcaaaacac
    33301 ggcgcaaaac gcgtaactgg ggtctggctc aaaattggcg cattttcttg tgtcgaaacc
    33361 agctctcttg ccttttgttt tgatctggtt tgccgcggca gcgtggcgga aggttgtaaa
    33421 ctgcacctcg aagaacaaga ggccgaatgc tggtgtgaaa catgccaaca gtatgtgacg
    33481 ctactgaccc agcgcgtccg ccgctgtcca cagtgtcatg gtgacatgct acagattgtg
    33541 gcagacgacg gtttacagat tcggcggata gaaatagacc aggagtgagc gatgtgtaca
    33601 acatgcggtt gcggtgaagg caacctgtat atcgagggtg atgaacataa ccctcattcc
    33661 gcgtttcgta gcgcgccatt tgccccggcg gcacgcccga agatgaaaat caccggcatt
    33721 aaaacgcctg aatttacccc cagccagact gaagaaggcg acctgcatta cggtcatggc
    33781 gaagcgggca ctcacgcgcc aggcatgagc cagcgccgga tgctggaagt cgaaattgac
    33841 gtgctggaca aaaacaaccg tctggctgaa cgcaaccgcg cgcgctttgc tgcccgcaag
    33901 caactggtgc tcaacctggt ttccagccct ggttccggta aaaccaccct gctgacggaa
    33961 accttaatgc gcctgaaaga cagcgttccg tgcgcggtta ttgaaggtga ccagcaaacc
    34021 gtgaacgatg ccgcacgcat tcgcgctacc ggcacaccag cgattcaggt gaacaccggt
    34081 aaaggctgcc atcttgacgc acagatgatt gccgacgccg caccgcgtct gccactggac
    34141 gataacggta ttctgtttat cgaaaacgtt ggcaacctcg tatgcccggc cagcttcgat
    34201 ctcggtgaaa aacacaaagt ggcggtgctt tccgttaccg aaggtgaaga caaaccgctg
    34261 aaatatccgc atatgtttgc cgccgcctcg ctgatgctgc tcaacaaagt tgacctgttg
    34321 ccgtatctca actttgacgt tgagaagtgc atcgcctgcg cccgcgaagt caatccagaa
    34381 attgaaatca tccttatttc cgccaccagc ggcgaaggga tggaccagtg gctgaactgg
    34441 ctggagacac agcgatgtgc ataggcgttc ccggccagat ccgcaccatt gacggcaacc
    34501 aggcgaaagt cgacgtctgc ggcattcagc gcgatgtcga tttaacgtta gtcggcagct
    34561 gcgatgaaaa cggtcagccg cgcgtgggcc agtgggtact ggttcacgtt ggctttgcca
    34621 tgagcgtaat taatgaagcc gaagcacgcg acactctcga cgccttacaa aacatgtttg
    34681 acgttgagcc ggatgtcggc gcgctgttgt atggcgagga aaaataatgc gttttgttga
    34741 tgaatatcgc gcgccggaac aggtgatgca gttaattgag catctgcgcg aacgtgcttc
    34801 acatctctct tacaccgccg aacgccctct gcggattatg gaagtgtgcg gtggtcatac
    34861 ccacgccatt tttaaattcg gcctcgacca gttactgcct gaaaacgttg agtttatcca
    34921 cggtccgggt tgcccggtgt gcgtactgcc gatgggcaga atcgacacct gcgtggagat
    34981 tgccagccat ccggaagtca tcttctgtac ctttggcgac gccatgcgcg tgccggggaa
    35041 acagggatcg ctgttgcagg caaaagcacg cggtgccgat gtgcgcatcg tttactcgcc
    35101 gatggatgcg ttgaaactgg cgcaggagaa tccaacccgc aaagtggtgt tcttcggctt
    35161 aggttttgaa accaccatgc cgaccaccgc tatcactctg caacaggcga aagcgcgtga
    35221 tgtgcagaat ttttacttct tctgccagca cattacgctt atcccgacgc ttcgcagttt
    35281 gctggaacag ccggataacg gtatcgacgc gttcctcgcg ccgggccacg ttagtatggt
    35341 tatcggcact gatgcctata attttatcgc cagcgatttt cagcgtccgc tggtggtcgc
    35401 tggtttcgaa ccccttgatc tactgcaagg cgtggtgatg ctggtggagc agaaaatagc
    35461 ggcccacagc aaggtagaga atcagtatcg tcgggtggta ccggatgccg gtaacctgct
    35521 ggcgcaacag gcgattgccg atgtgttctg tgtcaacggc gacagcgaat ggcgcggctt
    35581 aggcgtgatt gaatcttctg gcgtgcacct gacgccggat tatcaacgat tcgatgccga
    35641 agcacatttc cgcccggcac cgcagcaggt ctgcgatgac ccgcgcgcgc gttgtggcga
    35701 agtcttgacg ggcaaatgta agccgcatca atgcccgctg tttggtaaca cctgtaatcc
    35761 tcaaaccgcg tttggtgcgc tgatggtttc ctccgaagga gcgtgcgccg cgtggtatca
    35821 gtatcgtcag caggagagtg aagcgtgaat aatatccaac tcgcccacgg cagcggcggc
    35881 caggcgatgc agcaattaat caacagcctg tttatggaag cctttgccaa cccgtggctg
    35941 gcagagcagg aagatcaggc acgtcttgat ctggcgcaac tgatagcgga aggcgaccgt
    36001 ctggcgttct ccaccgacag ttacgttatt gacccgctgt tcttccctgg cggtaatatc
    36061 ggcaagctgg cgatttgcgg cacagccaat gacgttgcgg tcagtggtgc cattccgcgc
    36121 tatctctcct gtggctttat cctcgaagaa ggattgccga tggagacact gaaagccgta
    36181 gtgaccagca tggcagaaac cgcccgcacg gcaggcattg ccatcgttac tggcgatact
    36241 aaagtggtgc agcgcggcgc ggcagataaa ctgtttatca acaccgcggg catgggcgca
    36301 attccggcga atattcactg gggcgcacag acgctaaccg caggcgatgt attgctggtg
    36361 agcggtacac tcggcgacca cggggcgact atccttaacc tgcgtgagca gctggggctg
    36421 gatggcgaac tggtcagcga ctgcgcggtg ctgacgccgc ttattcagac gctgcgtgac
    36481 attcccggcg tgaaagcgct gcgtgatgcc acccgtggtg gtgtaaacgc ggtggttcat
    36541 gagttcgcgg cagcctgcgg ttgcggtatt gaaatttctg aatcagcgct gccggttaaa
    36601 cctgccgtgc gcggcgtttg cgaattgctg ggactggacg ccctgaactt tgccaacgaa
    36661 ggcaaactgg tgatcgccgt tgaacgcaac gcggcagagc aagtgctggc agcgttacat
    36721 tcccatccac tggggaaaga cgcggcgctg attggtgaag tggtggaacg taaaggtgtt
    36781 cgtcttgcca gtctgtatgg cgtgaaacga accctcgatt taccacacgc cgaaccgctt
    36841 ccgcgtatat gctaataaaa ttctaaatct cctatagtta gtcaatgacc ttttgcaccg
    36901 ctttgcggtg ctttcctgga agaacaaaat gtcatataca ccgatgagtg atctcggaca
    36961 gcaagggttg ttcgacatca ctcggacact attgcagcag cccgatctgg cctcgctgtg
    37021 tgaggctctt tcgcaactgg taaagcgttc tgcgctcgcc gacaacgcgg ctattgtgtt
    37081 gtggcaagcg cagactcaac gtgcgtctta ttacgcatcg cgtgaaaaag acacccccat
    37141 taaatatgaa gacgaaactg ttctggcaca cggtccggta cgcagcattt tgtcgcgccc
    37201 tgatacgctg cattgcagtt acgaagaatt ttgtgaaacc tggccgcagc tggccgcagg
    37261 tgggctatac ccaaaatttg gtcactattg cctgatgcca ctggcggcgg aagggcatat
    37321 ttttggtggc tgtgaattta ttcgttatga cgatcgcccc tggagcgaaa aagagttcaa
    37381 tcgtctgcaa acatttacgc agatcgtttc tgtcgtcacc gaacaaatcc agagtcgcgt
    37441 cgttaacaat gtcgactatg agttgttatg ccgggaacga gataacttcc gcatcctggt
    37501 cgccatcacc aacgcggtgc tttcccgcct ggatatggac gaactggtca gcgaagtcgc
    37561 caaagaaatc cattactatt tcgatattga cgatatcagt atcgtcttac gcagccaccg
    37621 taaaaacaaa ctcaacatct actccactca ctatcttgat aaacagcatc ccgcccacga
    37681 acagagcgaa gtcgatgaag ccggaaccct caccgaacgc gtgttcaaaa gtaaagagat
    37741 gctgctgatt aatctccacg agcgggatga tttagccccc tatgaacgca tgttgttcga
    37801 cacctggggc aaccagattc aaaccttgtg cctgttaccg ctgatgtctg gcgacaccat
    37861 gctgggcgtg ctgaaactgg cgcaatgtga agagaaagtg tttaccacta ccaatctgaa
    37921 tttactgcgc cagattgccg aacgtgtggc aatcgctgtc gataacgccc tcgcctatca
    37981 ggaaatccat cgtctgaaag aacggctggt tgatgaaaac ctcgccctga ccgagcagct
    38041 caacaatgtt gatagtgaat ttggcgagat tattggccgc agcgaagcca tgtacagcgt
    38101 gcttaaacaa gttgaaatgg tggcgcaaag tgacagtacc gtgctgatcc tcggtgaaac
    38161 tggcacgggt aaagagctga ttgcccgtgc tatccataat ctcagtgggc gtaataatcg
    38221 ccgcatggtc aaaatgaact gcgcggcgat gcctgccgga ttgctggaga gcgatctgtt
    38281 tggtcatgag cgtggggctt ttactggtgc cagcgcccag cgcattggtc gttttgaact
    38341 ggcggataaa agctccctgt tcctcgacga agtgggcgat atgccactgg agttacagcc
    38401 gaagttgctg cgtgtattgc aggaacagga gtttgaacgc ctcggcagca acaaaatcat
    38461 tcagacggac gtgcgtctaa tcgccgcgac taaccgcgat ctgaaaaaaa tggtcgccga
    38521 ccgtgagttc cgtagcgatc tctattaccg cctgaacgta ttcccgattc accttccgcc
    38581 actacgcgag cgtccggaag atattccgct gctggcgaaa gcctttacct tcaaaattgc
    38641 ccgtcgtctg gggcgcaata tcgacagcat tcctgccgag acgttgcgca ccttgagtaa
    38701 tatggagtgg ccgggtaacg tacgcgaact ggaaaacgtc attgagcgcg cggtattgct
    38761 aacacgcggc aacgtgctgc agctgtcatt gccagatatt gctttaccgg agcctgaaac
    38821 gccgcctgcc gcaacggttg tcgctcagga gggcgaagat gaatatcagt tgattgttcg
    38881 cgtgctgaaa gaaactaacg gcgtcgttgc cgggcctaaa ggcgctgcgc aacgtctggg
    38941 gctgaaacgc acgaccctgc tgtcacggat gaagcgactg ggaattgata aatcggcatt
    39001 gatttaacag atgtggttat actcgatcaa cattcaatga aaggaggcag tatggcagtt
    39061 tcagcaagaa atcaattaac cggaacagtt agcgcagtag caatgggcgc ggtgaatgac
    39121 gaagtagaac tgacgctggc aggtggtgca aagctggtgg caattgtgac tcacagcagc
    39181 cagcaggctc tgggtctggc gaaagggaaa gaagcgattg ccctgattaa agccccgtgg
    39241 gtaacgctgg caacggaaga ctgtggactg aaattctctg ctcgcaacca gtttgctggc
    39301 agcgtgtcga caatcaccga aggcgcagtg aacgccactg tccatatcaa aacggatgcg
    39361 ggttttgaaa ttgtggcagt ggtaacaaat gaaagtcagg atgaaatgaa actcaccact
    39421 ggctcacgcg ttattgcgtt gatcaaagca tcagcaatcc tgattgctac caaagcgtaa
    39481 gcgccttatt tgccggagag tatggctctc cggcagattt atttctttgg acgatatttt
    39541 tctggcaact ccggcaccgg acgcttgtca tcgatgagat gacgcacggt taagattgga
    39601 tgacgccaca gcattctcgg cccggcccaa cgcataatct gtttcatctc ttcacgcttt
    39661 gcaggctggt aacagtgcac cggacactgc ttacaggctg gtttctcttc gccgaacaca
    39721 catttatcca gccgcttttg cgcgtaaaca aacaacgcct cgtaatgctc cggctccgct
    39781 gacgcctgcg ggcatttcgc ttgataaaga tcgatcattt ttttaatcgt cagtttttca
    39841 cgagagatac gcttgccgga catactgcct ccatactctt aaggtgtatt tatactacaa
    39901 cttaatttga aaggggacta tggctctgaa ggaggaggtt tattcaaccg atacaatttt
    39961 gcgtacttgc ttcataagca tcactcaaaa gctgcaaaac agcatctttc ccggaaccag
    40021 catcaagaac tcgccgttcg cttcttcccc tgaaatgatt aactccggta tcatgtgcgc
    40081 cttatgtgat tacaacgaaa ataaaaacca tcacacccca tttaatatca gggaaccgga
    40141 cataacccca tgagtgcaat agaaaatttc gacgcccata cgcccatgat gcagcagtat
    40201 ctcaagctga aagcccagca tcccgagatc ctgctgtttt accggatggg tgatttttat
    40261 gaactgtttt atgacgacgc aaaacgcgcg tcgcaactgc tggatatttc actgaccaaa
    40321 cgaggtgctt cagcgggaga gccgatcccg atggcgggga tcccctacca tgcggtggaa
    40381 aactacctcg ccaaactggt gaatcagggc gagtccgttg ccatctgcga acaaattggc
    40441 gatccggcga ccagcaaagg tccggttgag cgcaaagttg tgcgtatcgt tacgccaggc
    40501 accatcagcg atgaagccct gttgcaggag cgtcaggaca acctgttggc ggctatctgg
    40561 caggacagca aaggtttcgg ctacgcgacg ctggatatca gttccgggcg ttttcgcctg
    40621 agcgaaccgg ctgaccggga aacgatggcg gcagaactgc aacgcactaa tcctgcggaa
    40681 ctgctgtatg cagaagattt tgctgaaatg tcgttaattg aaggccgtcg cggcctgcgc
    40741 cgtcgcccgc tgtgggagtt tgaaatcgac accgcgcgcc agcagttgaa tctgcaattt
    40801 ggcactcgcg atctggtcgg ttttggcgtc gagaacgcgc cgcgcggact ttgtgctgcc
    40861 ggttgtctgt tgcagtatgc gaaagatacc caacgtacga ctctgccgca tattcgttcc
    40921 atcaccatgg aacgtgagca ggacagcatc attatggatg ccgcgacgcg tcgtaatctg
    40981 gaaatcaccc agaacctggc gggtggtgcg gaaaatacgc tggcttctgt gctcgactgc
    41041 accgtcacgc cgatgggcag ccgtatgctg aaacgctggc tgcatatgcc agtgcgcgat
    41101 acccgcgtgt tgcttgagcg ccagcaaact attggcgcat tgcaggattt caccgccgag
    41161 ttacagccgg tactgcgtca ggtgggcgat ctggaacgta ttctggcgcg tctggcgttg
    41221 cgtactgctc gcccgcgcga tctggcccgt atgcgccatg ctttccagca actgccggaa
    41281 ctgcgcgcgc agttagaaaa tgtcgatagt gcaccggtac aggcgctgcg tgagaagatg
    41341 ggcgagtttg ccgaactgcg cgatctgctg gagcgggcaa tcatcgacac accaccagtg
    41401 ctggtacgcg acggtggtgt tatcgcaccg ggctataacg aagagctgga tgagtggcgg
    41461 gcgctggctg acggcgcgac cgattatctg gagcgtctgg aaatccgcga gcgtgaacgt
    41521 accggcctgg acacgctgaa agtcggattt aacgcggtgc atggttatta cattcagatc
    41581 agccgtggac aaagccatct ggcccccatc aactacatgc gtcgccagac gctgaaaaat
    41641 gccgagcgct acattattcc agagctaaaa gagtacgaag ataaagttct cacctcaaaa
    41701 ggcaaagcac tggcactgga aaaacagctt tatgaagagc tgttcgacct gctgttgccg
    41761 catctggaag cgttgcaaca gagcgcaagc gcgctggcag aacttgatgt gctggttaac
    41821 ctggcggagc gggcctatac cctgaactac acctgcccga cttttattga taaaccgggt
    41881 attcgcatca ccgaaggccg ccatccggtg gttgaacagg tgctgaatga gccgtttatc
    41941 gccaacccgc tgaacctgtc gccacagcgt cgcatgttga tcattaccgg tccgaatatg
    42001 ggcggtaaaa gtacctatat gcgccagacc gcactgattg cgctgatggc ctacatcggc
    42061 agctatgtac cggcacaaaa agttgagatt ggacctatcg atcgcatctt tacccgcgta
    42121 ggggcagcgg atgatctggc atccgggcgt tcaaccttta tggtggaaat gaccgaaacc
    42181 gccaatattt tacataacgc caccgaatac agtctggtgt taatggatga gatcgggcgt
    42241 ggaacgtcca cttacgatgg tctgtcactg gcatgggcgt gcgcggaaaa tctggcgaat
    42301 aagattaagg cattgacgct gtttgctacc cactatttcg agctgaccca gttaccggag
    42361 aaaatggaag gcgtcgccaa cgtgcatctc gatgcactgg agcacggcga caccattgcc
    42421 tttatgcata gcgtgcagga tggcgcggcg agcaaaagct acggcctggc ggttgcagct
    42481 ctggcgggcg tgccaaaaga ggtgattaag cgcgcacggc aaaaactgcg tgagctggaa
    42541 agtatttcgc cgaacgccgc cgctacacaa gtggatggta cgcaaatgtc tttgctgtca
    42601 gtaccggaag aaacctcgcc agcggtcgaa gctctggaaa atctcgaccc ggattcactg
    42661 actccgcgtc aggcgctgga atggatttat cgcttgaaga gtctggtgta ataacaattc
    42721 ccgatagaaa aatactatcg ggaatattat ctacagctga cgaatcaaat aaaaataccc
    42781 tgtataatag gaaagtttat tttacagggt aaaaccatgc catctacacg ctatcaaaaa
    42841 atcaatgccc atcactatcg ccatatatgg gtcgttggtg atattcatgg tgaatatcag
    42901 ttattacaat cccgcttaca tcaactctct tttttccccg aaaccgactt acttatttct
    42961 gtcggcgata atattgatcg tgggccggag agtcttgacg tcctgcgcct gctaaaccag
    43021 ccctggttta tctctgttaa aggcaaccac gaagcaatgg cgctggatgc gttcgcgacc
    43081 ggcgatggca atatgtggct tgccagcggt ggtgactggt tctttgagtt aaatgatatg
    43141 gaacaaaaag aagctatagg tctattgctg aaatttcatc aacttccaca tattattgaa
    43201 attaccaacg acattataaa atatgtcatc gcacatgcgg attatccggg caatgaatat
    43261 ctctttggca aacaaatagc ggagagcgaa tta
//
LOCUS       DAZGBC010000019        41463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGBC010000019 DAZGBC010000000
VERSION     DAZGBC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.019"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..1392
                     /gene="alaA"
                     /locus_tag="ACHK1J_001096"
     CDS             175..1392
                     /gene="alaA"
                     /locus_tag="ACHK1J_001096"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO7910402.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1476..2075
                     /gene="yfbR"
                     /locus_tag="ACHK1J_001097"
     CDS             1476..2075
                     /gene="yfbR"
                     /locus_tag="ACHK1J_001097"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7910403.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2134..3966)
                     /locus_tag="ACHK1J_001098"
     CDS             complement(2134..3966)
                     /locus_tag="ACHK1J_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO7910404.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4053..4703)
                     /gene="hxpA"
                     /locus_tag="ACHK1J_001099"
     CDS             complement(4053..4703)
                     /gene="hxpA"
                     /locus_tag="ACHK1J_001099"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO7910405.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLDQITVTKQPNGDVIIQ"
     gene            complement(4714..5208)
                     /locus_tag="ACHK1J_001100"
     CDS             complement(4714..5208)
                     /locus_tag="ACHK1J_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO7910406.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5291..5746)
                     /gene="yfbV"
                     /locus_tag="ACHK1J_001101"
     CDS             complement(5291..5746)
                     /gene="yfbV"
                     /locus_tag="ACHK1J_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO7910407.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6084..7286
                     /gene="ackA"
                     /locus_tag="ACHK1J_001102"
     CDS             6084..7286
                     /gene="ackA"
                     /locus_tag="ACHK1J_001102"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO7910408.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKDSGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7361..9505
                     /gene="pta"
                     /locus_tag="ACHK1J_001103"
     CDS             7361..9505
                     /gene="pta"
                     /locus_tag="ACHK1J_001103"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7910409.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9695..11215
                     /gene="yfcC"
                     /locus_tag="ACHK1J_001104"
     CDS             9695..11215
                     /gene="yfcC"
                     /locus_tag="ACHK1J_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO7910410.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVIFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11248..11790)
                     /gene="yfcD"
                     /locus_tag="ACHK1J_001105"
     CDS             complement(11248..11790)
                     /gene="yfcD"
                     /locus_tag="ACHK1J_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO7910411.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11848..12399)
                     /gene="yfcE"
                     /locus_tag="ACHK1J_001106"
     CDS             complement(11848..12399)
                     /gene="yfcE"
                     /locus_tag="ACHK1J_001106"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO7910412.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12455..13099)
                     /gene="yfcF"
                     /locus_tag="ACHK1J_001107"
     CDS             complement(12455..13099)
                     /gene="yfcF"
                     /locus_tag="ACHK1J_001107"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO7910413.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13235..13882
                     /gene="yfcG"
                     /locus_tag="ACHK1J_001108"
     CDS             13235..13882
                     /gene="yfcG"
                     /locus_tag="ACHK1J_001108"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO7910414.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13939..14301
                     /gene="folX"
                     /locus_tag="ACHK1J_001109"
     CDS             13939..14301
                     /gene="folX"
                     /locus_tag="ACHK1J_001109"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO7910415.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14322..15215
                     /locus_tag="ACHK1J_001110"
     CDS             14322..15215
                     /locus_tag="ACHK1J_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO7910416.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCTRWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15263..16153)
                     /gene="rpnB"
                     /locus_tag="ACHK1J_001111"
     CDS             complement(15263..16153)
                     /gene="rpnB"
                     /locus_tag="ACHK1J_001111"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO7910417.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLTAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16350..17123)
                     /gene="hisP"
                     /locus_tag="ACHK1J_001112"
     CDS             complement(16350..17123)
                     /gene="hisP"
                     /locus_tag="ACHK1J_001112"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO7910418.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGAIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17131..17847)
                     /locus_tag="ACHK1J_001113"
     CDS             complement(17131..17847)
                     /locus_tag="ACHK1J_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7910419.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17844..18530)
                     /gene="hisQ"
                     /locus_tag="ACHK1J_001114"
     CDS             complement(17844..18530)
                     /gene="hisQ"
                     /locus_tag="ACHK1J_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO7910420.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18620..19402)
                     /gene="hisJ"
                     /locus_tag="ACHK1J_001115"
     CDS             complement(18620..19402)
                     /gene="hisJ"
                     /locus_tag="ACHK1J_001115"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO7910421.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQKIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19623..20405)
                     /gene="argT"
                     /locus_tag="ACHK1J_001116"
     CDS             complement(19623..20405)
                     /gene="argT"
                     /locus_tag="ACHK1J_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO7910422.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20671..21240)
                     /gene="ubiX"
                     /locus_tag="ACHK1J_001117"
     CDS             complement(20671..21240)
                     /gene="ubiX"
                     /locus_tag="ACHK1J_001117"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO7910423.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21335..22852)
                     /gene="purF"
                     /locus_tag="ACHK1J_001118"
     CDS             complement(21335..22852)
                     /gene="purF"
                     /locus_tag="ACHK1J_001118"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO7910424.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22889..23377)
                     /gene="cvpA"
                     /locus_tag="ACHK1J_001119"
     CDS             complement(22889..23377)
                     /gene="cvpA"
                     /locus_tag="ACHK1J_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO7910425.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23818..24480)
                     /gene="dedD"
                     /locus_tag="ACHK1J_001120"
     CDS             complement(23818..24480)
                     /gene="dedD"
                     /locus_tag="ACHK1J_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO7910426.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24470..25738)
                     /gene="folC"
                     /locus_tag="ACHK1J_001121"
     CDS             complement(24470..25738)
                     /gene="folC"
                     /locus_tag="ACHK1J_001121"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO7910427.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25808..26722)
                     /gene="accD"
                     /locus_tag="ACHK1J_001122"
     CDS             complement(25808..26722)
                     /gene="accD"
                     /locus_tag="ACHK1J_001122"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO7910428.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26878..27537)
                     /locus_tag="ACHK1J_001123"
     CDS             complement(26878..27537)
                     /locus_tag="ACHK1J_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7910429.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27620..28432)
                     /gene="truA"
                     /locus_tag="ACHK1J_001124"
     CDS             complement(27620..28432)
                     /gene="truA"
                     /locus_tag="ACHK1J_001124"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO7910430.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRFDLPKPPMGPLFLAD"
     gene            complement(28432..29445)
                     /locus_tag="ACHK1J_001125"
     CDS             complement(28432..29445)
                     /locus_tag="ACHK1J_001125"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7910431.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29511..30647)
                     /gene="pdxB"
                     /locus_tag="ACHK1J_001126"
     CDS             complement(29511..30647)
                     /gene="pdxB"
                     /locus_tag="ACHK1J_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO7910432.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKKAGIGFSAAPGCNAIAVVEYVLSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRNLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHIALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30746..31741
                     /gene="flk"
                     /locus_tag="ACHK1J_001127"
     CDS             30746..31741
                     /gene="flk"
                     /locus_tag="ACHK1J_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO7910433.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31738..32916)
                     /locus_tag="ACHK1J_001128"
     CDS             complement(31738..32916)
                     /locus_tag="ACHK1J_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HFO7910434.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33225..34445)
                     /gene="fabB"
                     /locus_tag="ACHK1J_001129"
     CDS             complement(33225..34445)
                     /gene="fabB"
                     /locus_tag="ACHK1J_001129"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO7910435.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34604..36610
                     /gene="mnmC"
                     /locus_tag="ACHK1J_001130"
     CDS             34604..36610
                     /gene="mnmC"
                     /locus_tag="ACHK1J_001130"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO7910436.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHLHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEVPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIYYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDISEKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLVEQKDKAVSAPVYDDLFMLDALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36731..37009)
                     /locus_tag="ACHK1J_001131"
     CDS             complement(36731..37009)
                     /locus_tag="ACHK1J_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO7910437.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37043..37591)
                     /gene="epmC"
                     /locus_tag="ACHK1J_001132"
     CDS             complement(37043..37591)
                     /gene="epmC"
                     /locus_tag="ACHK1J_001132"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO7910438.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37591..38400)
                     /locus_tag="ACHK1J_001133"
     CDS             complement(37591..38400)
                     /locus_tag="ACHK1J_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO7910439.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38400..39224)
                     /gene="mepA"
                     /locus_tag="ACHK1J_001134"
     CDS             complement(38400..39224)
                     /gene="mepA"
                     /locus_tag="ACHK1J_001134"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO7910440.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKRVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39228..40313)
                     /gene="aroC"
                     /locus_tag="ACHK1J_001135"
     CDS             complement(39228..40313)
                     /gene="aroC"
                     /locus_tag="ACHK1J_001135"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO7910441.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40348..41280)
                     /gene="prmB"
                     /locus_tag="ACHK1J_001136"
     CDS             complement(40348..41280)
                     /gene="prmB"
                     /locus_tag="ACHK1J_001136"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7910442.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
BASE COUNT        10185 a        11285 c        10384 g         9609 t
ORIGIN      
        1 gcatatacac ctcattattt tgtcattatt aagttattaa cagcacaatc gagccttccc
       61 ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag tacattgttc
      121 taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc
      181 cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg
      241 aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac
      301 ccagccccgt tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg
      361 cctaccgctc aagggtattg cgattccaaa ggtctttact ccgcgcgtaa agccatcatg
      421 cagcactatc aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat
      481 ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg
      541 ttggttcctg caccagatta cccactatgg accgcggcgg tttcgctttc cagcggtaaa
      601 gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc
      661 gctaaaatta cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc
      721 gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc
      781 attatcttcg ccgatgaaat ttatgacaaa attctctacg acgacgctga gcatcactca
      841 attgcgccgc tggcacctga cctgctgacc attaccttta acggactgtc gaaaacgtac
      901 cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa
      961 ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg
     1021 caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct
     1081 ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaat tgatcaacga tattccgggc
     1141 gtttcctgcg tgaaacctcg tggtgcgctg tacatgttcc cgaaaatcga cgccaaacgc
     1201 tttaacattc acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg
     1261 ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta
     1321 ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg cgcgtttcct ttctggttat
     1381 catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagtgtt atcccgccac
     1441 aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc
     1501 atctctcccg cctgaaactc attaaccgct ggccgctcat gcgcaacgtg cggacggaaa
     1561 atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa
     1621 atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg
     1681 atgcctcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa
     1741 tcgctcagga gtacaaggct attgaaaaaa tcgctcagca aaaactggtc gatatggttc
     1801 cggaagagct gcgggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag
     1861 aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac
     1921 tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag
     1981 cgcgtcgcag ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt
     2041 cgctcgatga gattagccag gattcaccgc tgtaagcagc gggagtctgc gtcgcatcag
     2101 gcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat
     2161 caccacacaa accgccatca cgataatggt gaacggtacc cccagcttca caaagtcact
     2221 gaagctgtaa tttcccggac ctaaaaccag tgtgttaacc ggtgaagaaa ccggtgtcat
     2281 aaaggcggcg gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat
     2341 cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat
     2401 aaatagccca ataaccgccg acaagacaaa caaacaaccc agcatcatat gtggcccgta
     2461 accaccgcca atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa
     2521 tgccacagca aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt
     2581 ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc
     2641 ggcaacagga ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa
     2701 aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt
     2761 cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac
     2821 aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac
     2881 gccattgcgc tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg
     2941 caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc
     3001 aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact
     3061 acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc
     3121 gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc
     3181 aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc
     3241 aatcattggc gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc
     3301 acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc
     3361 ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag
     3421 gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact
     3481 gttgactacc aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa tcagcccggc
     3541 aaaacttaac ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg caacgcttaa
     3601 caccacggga ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc
     3661 gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcaa ctttgaccag
     3721 ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa
     3781 ggcggcaatc aggacaacgt taggatcaga aaagccggaa aatacttctg ggactgtcag
     3841 cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct
     3901 gcccgtcgca aacaagacga tggcaacggc cagtaatgaa agaacccaaa tcaattcacc
     3961 gttcacaact tatccttgtt aattgagggg gatgacttga ttctgccata aaaaagcccc
     4021 gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc
     4081 tgtttggtca cagtaatttg atccagactg tggaggacca aatcgacctc attcaggcgc
     4141 ggggtatctg ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc
     4201 acgccagcgg gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgt
     4261 gcgcctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggtcaca
     4321 aacacctctg gcgcgggaag ccccgctatt ttatggcgcg ctcgcgctac cggcatggag
     4381 ccagaagtca caatggccca cggaatacct gctttattca aatgattgag taaggcaatt
     4441 gcccccggaa gcgcggtaat accttcggtt tccgtggcct cgatttgctc cagacgcgta
     4501 aactcggcgg caatatcagc ctcggatttg cccgccataa aatggcgcag agaggtgatc
     4561 gcctgtttac cgtgaatgaa agccagcacc tcttccggcg ctaacccatg acgtctggcc
     4621 cagttgctcc acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca
     4681 aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc
     4741 gttggcgctc aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata
     4801 tttttcccac attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata
     4861 gcgcccttcc acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc
     4921 gtcaaagccg aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga
     4981 ccaggaaaca tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc
     5041 ttctttcagc tcgccaaact cgcgatccag ttcacgcatc tgtaatccat aaccacgctc
     5101 aatgattgtt tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat
     5161 cttgtactgg ttagacaaaa taagacgttg cgcgttggtc atttccattg ttgactcctg
     5221 tatcactcta ttacggtgaa aaaaaagaag gctgagtatg ccttctttta tatgcgtaat
     5281 caggggtcaa ttacaaatca tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa
     5341 gcgtgtcagc taatgcctgg taatcaggct tgccttcaac gggtgccaac acctgtccag
     5401 actcctgcaa tttaccgcga acttcataaa accagttgag gattgcaggg ggtaatggcg
     5461 tgacagaacg cttgcccagc caccacaatc cctgcatggg taaacttaag gcgaacaggg
     5521 cagtggcaac tgccggccca agctgaccgc ccagggcaat ctgccagcag agagtaaata
     5581 cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat cttgataacg cgattttcga
     5641 caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc tgtccccggc
     5701 gaaacaagct aaaaaaatta acagaacgat tatccggcgt tgacatgctt cacctcaact
     5761 tcacatataa agattcaaaa atttgtgcaa attcacaact cagcgggaca acgttcaaaa
     5821 cattttgtct tccataccca ctatcaggta tcctttagca gcctgaaggc ctaagtagta
     5881 catattcatt gagtcgtcaa attcatatac attatgccat tggctgaaaa ttacgcaaaa
     5941 tggcatagac tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg catgatgtta
     6001 atcataaatg tcggtgtcat catgcgctac gctctatggc tccctgacgt ttttttagcc
     6061 acgtatcaat tataggtact tccatgtcga gtaagttagt actggttctg aactgcggta
     6121 gttcttcact gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac ctttctggtt
     6181 tagccgaatg tttccacctg cccgaagcac gtatcaaatg gaaaatggac ggcaataaac
     6241 aggaagcggc tttaggtgca ggcgccgctc acagcgaagc gctcaacttt atcgttaata
     6301 ctattctggc acaaaaacca gaactgtctg cgcagctgac tgctatcggt caccgtatcg
     6361 tacacggcgg cgaaaagtat accagctccg tagtgatcga tgagtctgtt attcagggta
     6421 tcaaagatgc agcttctttt gcaccgctgc acaacccggc tcacctgatc ggtatcgaag
     6481 aagctctgaa atctttccca cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt
     6541 tccaccagac tatgccggaa gagtcttacc tctacgccct gccgtacaac ctgtacaaag
     6601 agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta acccaggaag
     6661 cggcaaaaat gctgaacaaa ccggtagaag aactgaacat catcacctgc cacctgggca
     6721 acggtggttc cgtttctgct atccgcaacg gtaaatgcgt tgacacctct atgggcctga
     6781 ccccgctgga aggtctggtc atgggtaccc gttctggtga tatcgatccg gcgatcatct
     6841 tccacctgca cgacaccctg ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag
     6901 attctggcct gctgggtctg accgaagtga ccagcgactg ccgctatgtt gaagacaact
     6961 acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat
     7021 acatcggtgc ctacactgcg ctgatggatg gtcgtctgga cgctgttgta ttcaccggtg
     7081 gtatcggtga aaatgccgcg atggttcgtg aactgtctct gggcaaactg ggcgtgctgg
     7141 gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca
     7201 acaaagaagg tacccgtcct gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc
     7261 aagacgcgag ccgcctgact gcctgatttc acaccgccag ctcagctggc ggtgctgttt
     7321 tgtaacccgc caaatcggcg gtaacgaaag aggataaacc gtgtcccgta ttattatgct
     7381 gatccctacc ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat
     7441 ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc gtaccggtgg
     7501 cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac tcttccacca cgacggccgc
     7561 tgaaccgctg aaaatgagct acgttgaagg tctgctttcc agcaatcaga aagatgtgct
     7621 gatggaagag atcgtcgcaa actaccacgc taacaccaaa gacgctgaag tcgttctggt
     7681 tgaaggtctg gtcccgacac gtaagcacca gtttgcccag tctctgaact acgaaatcgc
     7741 taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca
     7801 gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat
     7861 caccggcgtt atcgttaaca aactgaacgc accggttgat gaacagggtc gtactcgccc
     7921 ggatctgtcc gagattttcg acgactcttc caaagctaaa gtaaacaatg ttgatccggc
     7981 gaagctgcaa gaatccagcc cgctgccggt tctcggcgct gtgccgtgga gctttgacct
     8041 gatcgcgact cgtgcgatcg atatggctcg ccacctgaat gcgaccatca tcaacgaagg
     8101 cgacatcaat actcgccgcg ttaaatccgt cactttctgc gcacgcagca ttccgcacat
     8161 gctggagcac ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct
     8221 ggttgccgct tgcctggctg ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg
     8281 cggctacgaa atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct
     8341 gccggtattt atggtgaaca ccaacacctg gcagacttct ctgagcctgc agagcttcaa
     8401 cctggaagtt ccagttgacg atcacgagcg tatcgagaaa gttcaggaat acgttgctaa
     8461 ctacatcaac gctgactgga tcgattctct gactgccact tctgagcgca gccgtcgtct
     8521 gtctccgcca gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat
     8581 cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca gccgctatct gtgctgaacg
     8641 tggtatcgca acttgcgtac tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc
     8701 tcagggtgta gaactgggtg caggcattga aatcgttgat ccagaagtgg ttcgcgaaag
     8761 ctatgttggt cgtctggtcg agctgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg
     8821 cgaacagctg gaagacaacg tggtgctcgg tacgctgatg ctggaacagg atgaagttga
     8881 tggtctggtt tccggtgctg ttcacactac cgcaaacacc atccgtccgc cgctgcagct
     8941 gatcaaaact gcaccgggca gctccctggt atcttccgtg ttcttcatgc tgctgccgga
     9001 acaggtttac gtttacggtg actgtgcgat caacccggat ccgaccgcag aacagctggc
     9061 agaaatcgcg attcagtccg ctgattccgc tgcagccttc ggtatcgaac cgcgcgttgc
     9121 tatgctctcc tactccaccg gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga
     9181 agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta
     9241 cgacgctgcg gtaatggctg acgttgcgaa atctaaagcg ccgaactctc cggttgcagg
     9301 tcgcgctacc gtgttcatct tcccggatct gaacaccggt aacaccacct acaaagcggt
     9361 acagcgttct gccgacctga tctccatcgg gccgatgctg cagggtatgc gcaagccggt
     9421 taacgacctg tcccgtggcg cactggttga cgatatcgtc tacaccatcg cgctgactgc
     9481 gattcagtct gcacagcagc agtaatctcg tcatcatccg cagctttgcg ctgcggatat
     9541 ctgaaccgga aatagtcact atttccggtt ttttattctc ttaatttgca ttaatccttt
     9601 ctgattatct tgcttaactg cgctgcatca atgaattgcg ccatttcact ttgcatactt
     9661 accactttgt tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg aatccaaacc
     9721 aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg ctattttaac
     9781 cagccttgcc acctgggtag ttccggtggg gatgtttgac agtcaggaag tgcagtatca
     9841 ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca cactcatttc gcattctgac
     9901 taacgaagca ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg
     9961 cccgggcctg atgaacttcc cgtttgaagg gttaacctca ggatcgaaat acgggacagc
    10021 cgttggcatc atcatgttta tgctggtgat tggcggcgcg tttggcattg tgatgcgtac
    10081 aggaaccatt gataacggta tcctggcgct tattcgccat acccgcggga atgaaattct
    10141 ctttattcct gcgctgttta ttctgttttc acttggcggc gcggtatttg gtatgggaga
    10201 agaggccgtc gcctttgcca ttatcatcgc accgctaatg gtccggctgg gctatgacag
    10261 tattaccacc gtcctggtga cctatattgc cacgcaaatc ggttttgcca gttcgtggat
    10321 gaacccgttt tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc tttctggctc
    10381 cgggttgcgc atcgtggtgt gggttatcgc cactctgatt ggcctgatct ttaccatggt
    10441 gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt
    10501 ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt acctttggtg actggctggt
    10561 attgattgtc ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg
    10621 gtttattcca gaaattgcca gccagttctt caccatgggt ctggtgattg gcatcatcgg
    10681 cgttattttc cgccttaacg gcatgacggt taataccatg gcttcatcct ttaccgaagg
    10741 ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc gccaaaggga ttttgctgct
    10801 ggttggtaat ggtgaagcag gtgatgccag cgtgttaaat actatcctca acagcattgc
    10861 caatgccatt agcggtctgg acaacgcggt cgcggcctgg tttatgttgc tcttccaggc
    10921 ggtatttaat ttcttcgtga cgtccggttc tggtcaggcg gcgttaacca tgccgttact
    10981 ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg cgttccagtt
    11041 tggtgatggc ttcagtcaca tcatttaccc gacctcagct tcgttaatgg cgacgctcgg
    11101 tgtttgccgg gtggacttcc gtaactggct gaaggtgggc gcgaccctgc ttggactgct
    11161 gtttattatg tccagcgtcg tagtgatcgg cgctcagttg atgggctacc actaaaaatg
    11221 ttaagagccg caattgcggc tcttttttca ttctgccgtt tcagtctcta cggcttcatt
    11281 tttggcattg cgcttcatcc acaacgctag cgctttcagc gagtctggag tgaactcatc
    11341 gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact tcactgactt catcttcctg
    11401 tagggcgaag ggaccgtgag agacgcagct gaacaatgcg ccccagacac ggcaattttt
    11461 atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca ccggcaatgc ccaactcttc
    11521 ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg gcctgaacca caccgcctgc
    11581 ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat
    11641 tttgcccatg ccatcatgca cgacgatgta agttgcacga tgacgcagac actgtgcccg
    11701 catttgctcc cggctggctt gtgcaatgac ttcgttctct tcattgacaa tatccaccca
    11761 ttcagtactt gccaaacgac gctgttccat tatcaggaaa ccttcttttt ctggcgctct
    11821 tacggcgcat tttgagttgt gggtaaatta cggattaatc gcgacctgcg caatgatact
    11881 ttgatcattg agtgcgatta cactaagtac gtcattatcc agcatgccat aactcgccgg
    11941 attaccgcct ttcggaatac tcaccgagcc ggggttgaag tggaaaatct ccccccgctg
    12001 ttctgctact ggcagatggg tatgaccgta aaccagcaca tcgttctggt ttaaagcagg
    12061 cagattttcc gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa
    12121 tacctgttgc cacggcgcgg ttatcgggaa atgcagcagc atttgatcca cttcgctgtc
    12181 gcagttgccg cgcacagcga taaccttatg tgccacttca ttaagccgtt cagcgacttt
    12241 ggctggcgcg taaccctccg gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag
    12301 gatcaccagc cactgggcac cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg
    12361 taacgaccca tgaatgtccg atgcaaacat cagtttcatc actcactcct cgtcgaaaaa
    12421 acgtaagcta tgatactgga ttctgtcgtt gctatcagcc agattgcttc gccgaaagtg
    12481 caataaaacg ctggacagac gctcgctgcc actggaatgt tgcataatcc accaggcgtt
    12541 ctggcacctc atcgccatgt agtaccaggc ggttaatcat tagcgccaga tcagtatcag
    12601 caatgcacca ttcaccaaat aaattcggct gacccagtac taacaaatgt tctgccatcg
    12661 cgaacagttt ctctgcactg gcttttccct cggccgttag tggcgctttt ttcgcccccg
    12721 caaagacaac atccgtcgga cgctcttcgc ggatgggcat cagatcgctg cgcaaccagg
    12781 cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag cggataaata cgttcccagg
    12841 tcggtggcgc aaatcgatct tccagatact ccgcaatggc agaagattca ctcaactcaa
    12901 aatcatcgat ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc cacgtcggtt
    12961 gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg aaacgacagg cctttttcct
    13021 gcaacgccac ccaggcggat aacacataag gggagaaaca gtgggcatct gaccaaagcg
    13081 tgatagcggg tttactcatt atgtcctcgg caataaaggg ggctggctat caaactatag
    13141 agcctttttg tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct ttctatactt
    13201 gtggtgttca gatgattaga caaccggagc tgcaatgatc gatctctatt tcgccccgac
    13261 acccaatggc cacaaaatta cgctgtttct cgaagaagca ggtctggatt atcgcttgat
    13321 aaaggtagac ctggggaaag gcggtcagtt tcgcccggaa tttttgcgca tttcgcctaa
    13381 caacaaaatt ccggcaattg ttgatcattc tccagccgat ggcggcgaac cgctaagcct
    13441 ctttgaatct ggtgccattt tgttgtatct ggctgagaaa acggggctct ttttgagtca
    13501 tgaaacgcgt gaacgcgccg ccacattaca gtggttattc tggcaggtag gcggactggg
    13561 gccgatgctt gggcaaaatc atcattttaa tcacgcagcc ccccaaacca ttccttacgc
    13621 tattgaacgt tatcaggttg aaactcagcg tctgtaccat gtactgaaca agcggctgga
    13681 aaactcgccc tggctgggag gcgagaacta cagcattgcg gatattgcct gctggccgtg
    13741 ggttaatgcc tggactcgcc agcgaattga cctcgcaatg tatccggcag tcaagaactg
    13801 gcatgagcgg atccgttcgc gccctgccac cgggcaggca ctgctaaaag cacaactcgg
    13861 tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg tattatttca tcctaagtaa
    13921 aacaacggag aacctgcaat ggcacaacct gccgctatta ttcgtataaa gaaccttcgt
    13981 ttacgtacgt ttatcggaat taaggaagaa gaaattaaca accgtcagga tattgttatc
    14041 aatgtgacga tccactaccc cgccgataaa gcgcgcacta gcgaagatat caacgatgcg
    14101 ctgaattatc gcaccgtaac gaaaaacatt attcagcatg tagagaataa ccgtttctct
    14161 ttgctggaaa aattaactca ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg
    14221 tatgctgaag tggagatcga taaactgcac gcgctgcgct acgccgattc ggtatccatg
    14281 accttaagct ggcagcgtta accgccatat cgggaggctg catgaacata gtgatcaccg
    14341 gagggacggg attaattggt cgccatttga ttccacgttt gctggagctg ggccatcaaa
    14401 tcacggtaat gacgcgtaac ccgcagaaag ccagttccgt tctcggccct cgggtgacac
    14461 tatggcaagg gcttgccgat caaagcaacc tcaacggcgt tgatgcggta atcaacctgg
    14521 ccggagaacc gattgctgat aaacgctgga ctcacgagca aaaagagcgt ctctgccaaa
    14581 gccgctggaa tatcacgcaa aaactggtcg atttgattaa tgccagcgac acgccaccgt
    14641 cggtactcat ttccggctcg gcaacgggct attatggcga tttaggtgaa gtggtggtta
    14701 ccgaagagga accgccgcat aacgaattta cccataaact ctgcacccgc tgggaagaaa
    14761 ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgtacc ggcgtagtgc
    14821 tggcaccgga tggcggtatt ctcggtaaaa tgctgccgcc gtttcgtctt ggcctgggcg
    14881 ggccgattgg ttccggtcgg cagtatctgg cctggattca tatcgatgat atggtcaacg
    14941 gcattctctg gctgctggat aacgagctgc gcgggccatt taatatggtt tcgccttacc
    15001 cgatacgcaa tgaacaattt gcccatgcgc tcggtcatgc gctgcatcgc ccggctattt
    15061 tgcgcgtccc cgcaaccgcc attcggctgt taatgggcga atcttcagta ctggtattag
    15121 gtggacaacg cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt cgttggtacg
    15181 atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg tttactgcaa acatccgcca
    15241 gttaactccc ggtgttacag ggttagtggc tttgcgcgat aagatcgtct ggtgaaagtc
    15301 gggtcagcgt caggatgact tcgtggtcaa atcctttttc cagcatctcc tgagcaatac
    15361 gcagagcttc ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg tcagcaatgg
    15421 tcatcagttt ctccttttct tgtggtgcgc gttctgttat ctcaccaata aatgcacgga
    15481 aacgctgggc atctcccgtt tgtaatacat aattaaacag ggcttttagc tgtctgtcat
    15541 tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct
    15601 gacgaatatg tttctgaatt aactccaaca gcgccatttt gcgatgttgc ataatctcgt
    15661 catccggcac cacggtaata tccaccaacg gaaaagccga tgaatatatt ttacgggcaa
    15721 tcgcaggctc ggcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac
    15781 catgataaaa cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt
    15841 tttgcatcgc cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac
    15901 tttggtgctc tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca
    15961 agaggtcgga ataatattgc cgcaggtctt catcaataaa actgtttggt tccagtttaa
    16021 gcgtcgttaa atcacacagt ttgcgcagcg gcgcggtaag atgaatatca ataaaatccc
    16081 gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg
    16141 tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg
    16201 attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt
    16261 agcggactgc aacttttaca tttcagcgga agctgactcg caaattcccc ttgaaaatac
    16321 tccggcagcg ttagccaccg gagaggcaat tatttcagcg aacctttaag gaactgttgc
    16381 agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccttcttc ctctattttc
    16441 ccctgatgga ggaaaatgac gtgagtagaa acatgacgag caaagcccat ttcgtgagtc
    16501 actaccacca tggttttccc ctcttctgcc agctgctgca taatgcgcaa cacttcgcct
    16561 accagttcag gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc
    16621 gccagcgccc gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat
    16681 ttcccctgtg cacgttcgtc tatcccgact tttgccagat acttcaccgc ccgctcgcgc
    16741 gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc
    16801 gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag
    16861 cgcagttgat ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac cagattgatc
    16921 gtctggccat tgaccacgat cgccccttca ctcggttttt cgaggaagtt aatgcagcgc
    16981 agaaaggtac ttttccccga tcccgacgat ccaatgatgc ttattacatc tccggcattc
    17041 gcttgcagtg acaccccttt cagcacttcg tgttcgccat agcgtttgtg caaatcgata
    17101 acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg
    17161 caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata
    17221 gagcaccgcg gcaatgccga aggcagtaaa aggttgatac gtggcggcgt taatatcgcg
    17281 ggctattttc agcagatccg gcaccgtggc agtaaatgcc aacgcagtag agtgcagcat
    17341 caggatcact tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat
    17401 gcagcgatac attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg
    17461 cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt
    17521 cagcgccagc acggtacagt tcaggccact gcggaaaaag gcgttaagaa actcggtccc
    17581 cttaacaatc tccagcgtat acatgccgga atagaacacc agcaactgaa catacagcgg
    17641 cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga
    17701 gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag
    17761 cagccacagg gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt
    17821 ccagtattca tgtaagattt cgatcacaga tcagccctct tcacacccac ggagtagcgg
    17881 cgctcaagga acagcagcac accattggaa acggtggtga aaaccaagta aatcacgcca
    17941 cagacgatgg cgaaatagaa cggttcccag gtacttttgc ctgccagctg cgtggctttg
    18001 accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag
    18061 ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac
    18121 acttgcccac gagtaaaacc gaacgccgtc gccgcctcta tatgtccttt cggcactgcc
    18181 ataaaagcac cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtgata
    18241 ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg
    18301 tttagcgcaa tctgcaaacc gtagaaaatc agcagcatca acactaagtc cggcacgcca
    18361 cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta
    18421 ccgccagcgc caattaaacc gatgattaca gcgagcacta cagagctgat agccagctcc
    18481 agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc
    18541 ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc
    18601 tttctttcac cgatggcgat taaccaccat aaacatcaaa atcgaagtac tttttcgcta
    18661 atttctcgta agtaccgtca gctcgcattt cggcaaaggc tttgttcagt gcctcgcgca
    18721 gttcgttatc ttctttacgc aggcccatgc cggtccctac gccaaacagt ttttcatctt
    18781 taacagacgg gccaccgaat ttgtaatctt taccgacagg ttgtttgagg aaaccttcgc
    18841 tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa
    18901 tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccag tgttcgttac
    18961 cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg tttgcctttc agcgactcga
    19021 ctgtcggctg aatgtcagaa tttttcgcca ccaccagacg agaatcggca gcgtacagtt
    19081 tgtcggtgaa ggctatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg
    19141 cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg
    19201 tacattgcgt attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca
    19261 gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga
    19321 ttttttgcgg aatcgcagca aatgccgcag ttgcgctgga gaaggccaga accagagaga
    19381 gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata gggaatattt
    19441 acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat atctcgcccc
    19501 gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag
    19561 tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt ccctgcgacg
    19621 tatcagtcac cgtagacatt aaagtcaaaa tactttttcg ccatcttgtc gtaggtgccg
    19681 tcctgacgca gctctccaag cgccttattg aaagcagccg tcagttcagc atcatcttta
    19741 cgtagcccta caccggtgcc atcaccgaag tattttttgt ctttcactga tgagccagca
    19801 aaggcgaagt ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatcc
    19861 tgcagtgcag catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca
    19921 taggccacca catccacacc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt
    19981 gatccctgca gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggc
    20041 gaacctttgg cggcaatcag acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc
    20101 tcctgctgac gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct
    20161 ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc
    20221 cgtttgcaca tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct
    20281 ttcgacgaga acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc
    20341 gcatagctgg aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc
    20401 ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt
    20461 gcaggctatt tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa
    20521 atgtgttaat aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg
    20581 aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca
    20641 aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc
    20701 ttcaggaagg gttatcgcaa actggtcaag aacacgatta accgtctgat ttatcacatc
    20761 atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat
    20821 ttctgctgcc tgagtcatta aacgcagatg acctaagtgc aatggtgttt cacgcacgca
    20881 gagcaccaac gggcgacgct ctttcagcac aacatctgcc gcacgggtca gtaagccatc
    20941 agtatagcta tggacaatgc cggacagggt tttgattgaa cagggtaaaa tcaccatccc
    21001 cagcgtctgg aaagaaccgg aagagatgct ggcggcaata tcgcgcgcat catgcgtgac
    21061 atcagctaat gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg
    21121 cgccgcctgg ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa
    21181 taaacgcacg ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat
    21241 aaatagttgc cctgtcagac ttgcaggcag actttgcagg atttcggcgg gagttgcaag
    21301 tcagggtgcc agaccggcac cctgagcgaa ggcatcatcc ttcgttatgc atttcgagat
    21361 tttccacttc gttctgacgt tgcactgctt tggcgtcgtc attacgtaac gtatcgagga
    21421 aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac accgaacatt
    21481 caaactgctg aatatccggg ttttcagcgc gaacggcttc gatcagatcg ttcagatcct
    21541 ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atctacttcg cgaccgtgag
    21601 cgatcagttc cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg
    21661 gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct
    21721 gctcagaagt ggtgccacgg acgatggagt cgtcgaccag caggacgttt ttatcgcgga
    21781 actcggcgcg gttggcgttc agtttacggc gcacggactt acgacgcagc tgctggcccg
    21841 gcatgatgaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggcttgc
    21901 ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttccgggatc gggatcacca
    21961 cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat
    22021 tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata
    22081 catactcaaa caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact
    22141 gcccttcttc agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg aaatcaaatc
    22201 ccagcgtatc gagcgcaacg ctttcggaag cgaccatata ttctgtacgg ttctcgtcaa
    22261 tatcacgttt tcccagtacc agcggacgaa taccgtttgg atcgcggaaa gcaaccatac
    22321 cgtggccgat aatcatcgcc acacaagcat acgcgccgcg gattaagcgg tttgtagcag
    22381 cgatggcggc gaaaatgttg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc
    22441 tggcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt
    22501 cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg
    22561 taatgccata cggggagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag
    22621 ccgtggggta acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag
    22681 cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat
    22741 ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat
    22801 aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt
    22861 ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt
    22921 gcagataatc aaaaaaccat ctgatgataa aactgaactg cgggatcagc tgtgatttac
    22981 tccagtcttc gcttttcgac accccggtaa aggagtcgag aaagaagaga atggcagcaa
    23041 caatcaacac accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca
    23101 accccgtttt ctccaccagc tggcctatca cgaagttcac gatagcacca acgatcaggg
    23161 tagcgataaa cagtaccgcg atggcaatcc catttcgaac cagttcgtct tcaaagcccg
    23221 taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg
    23281 tcaccagcga taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag
    23341 caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc
    23401 tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct
    23461 tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc
    23521 tttgcggcat cggacaatgc gtgtcggatg cggcgtaaac gccttatccg acctacggtt
    23581 ctaaccctgc gtaggcctga taagacgcgc cagcgtcgca tcaggcaacg gctgtcggat
    23641 gcggcgtgaa tgccttatcc gacctacggt tctacccctg cgtaggcctg ataagacgca
    23701 ccagcgtcgc atcaggcaac ggctgtcgga tgcggcgtga acgccttatc cgacctacgg
    23761 ttctacccct gcgtaggcct gataagacgc gccagcgtcg catcaggcaa gaccgtatta
    23821 attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca actcacccag
    23881 cgatcctttc agcttatctt tcgaggcatc tggcccaacc agaatacggg taattttacc
    23941 ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac
    24001 aatctcattc actttatcgg catttttcag cgcccccagt tgcacaacat aggctttacc
    24061 cgtcggtgca gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtgc
    24121 ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga ccggtgcagg
    24181 ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc
    24241 ggcatcacct gcccgcacct cttccgctgc gccttccggc ggttgcgtcg gcaacgcctg
    24301 ggtggcggct ggcatcatat caggctcatc gcgatcgccc gcttttggca ccagcgggat
    24361 agccgcaaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat
    24421 cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac
    24481 cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaac gaaccacaca
    24541 ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgcaacgc
    24601 tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg
    24661 gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc
    24721 cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta
    24781 gcgttttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa
    24841 taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc
    24901 cgtcgcgaat ggcattttca ctgacctcca gcccgctggc acgcagtgcc gccagcgccg
    24961 ttgcggcatt cggttgcggg acaagcggca acggcaggtt ttccagcgtg ccgtgagcat
    25021 cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta
    25081 acagtgcacc tttttcctgc gctacatcag caatggtaga aggcatttcc ggctcaccga
    25141 caattgccgg tttttcgctg cgaaagatgc ctgctttctc gcgaccaata ctttcgcgat
    25201 ctggccccag ccagtcggta tgatccagcg caatgctggt tactaccgcg acatcggcgt
    25261 cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc accacgtcaa
    25321 gttgtgcctg tttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg
    25381 aaatatcacc gcgtgccgat tcaatctccg caaaagaggc agtgtgggcc gattccggca
    25441 attcctgacc ctgcacacgt acgcgctcgg tataacgcac cagatgcggc gaactgtaga
    25501 cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt
    25561 tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcagaacg ccaagacgcg
    25621 ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca
    25681 gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg
    25741 tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta
    25801 tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg ccttcacgcg
    25861 gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca
    25921 ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac
    25981 tgcgctggaa tcctggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg
    26041 caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg gcgaaacttg
    26101 cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct
    26161 gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct
    26221 gcatacgtgc gccaccagag gcggagaagc agatcagtgg gcagttatct tccagcgcct
    26281 gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg cccataaagg
    26341 cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc atcaccacca
    26401 gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta tacttcttgg
    26461 agtcacgaaa cttcagcaca tctttcggct caagctcgct acccagctcc acaaggcttc
    26521 cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact
    26581 tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat
    26641 cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt
    26701 taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc gatgccagtt
    26761 ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt
    26821 aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta
    26881 ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc ccggcaaaat
    26941 agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca tcgggattgt
    27001 accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc cgatcacgtt
    27061 ataagcggcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg gggcgaacgt
    27121 tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcataaaa
    27181 ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat tactgaataa
    27241 cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa tcgcggcgat
    27301 cagcatcagc accaccatca tatggacgtt gagatcgttg gtttccagtg atgccaacgc
    27361 tccggcaaca aacagcaatg aatcacccgg taaaaacggc gttaccacca gaccggtttc
    27421 acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg cgaccagctc
    27481 tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt
    27541 tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat
    27601 gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa
    27661 gatcaaaccg gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg
    27721 ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt
    27781 tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg
    27841 cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta atatgcataa
    27901 cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct
    27961 cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat
    28021 gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg atatagcgat
    28081 aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac
    28141 gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa cgtccacgca gcgtctttgc
    28201 gctgcgcggt tgtttcgaaa tgcaccacct gcccggtacc gtgtacccct gcgtcagtac
    28261 gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct
    28321 tctcctgcac actgcgaact tcgttctgcc gttgccagcc gtaatactta ctgccgtcgt
    28381 actcaatgcc cagcgcaatt ttataaactg gcggttgttg ctggtcggac attagtacag
    28441 atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac
    28501 gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat tacgcacgca
    28561 gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagttggga attcgttctc
    28621 ttcagagagc acaatatctt cgccttgaac aaacgcatca cgcgcttctt ctgctgccag
    28681 tggacgcaga gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac
    28741 gacgcttgcc gaaatcatca gtccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat
    28801 acgacgttct tcacgcacgc taccttcgct atccggcagt aacggcagca tgttgaacgc
    28861 cagctgacgc ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca atttagcact
    28921 ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt
    28981 aacgctgata cgtgataaac cgccctgatc gattaacggt ttcagtgccg ccagcagctg
    29041 gctggtcaga ctgtccggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa
    29101 cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca atccgctgct
    29161 gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggctgcgg tagcttcttt
    29221 gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcag catcctgcac
    29281 ggtgattgtc ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc
    29341 ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccac
    29401 agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag
    29461 aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga ttaacgtgcc
    29521 ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact ggcgtcatca
    29581 caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg cagtttatcg
    29641 aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac
    29701 accaaatgca ccagcctttt cagcgttggt tgatcgagcg ggccatgcag cgtaatgcga
    29761 ccaaactctg gcgcgggcag taatgtatcc agcgcaatgt gctgttcatg cccaataaac
    29821 ttgctatagg cttcaaacac ttgcgtagta ccgcgtgctt taccttccag ggtatagcct
    29881 gcaatatgcg gcgtgccgat atccactttt gtcagcaact ccacgttaag ttccggttcg
    29941 ccttcccaga catccagcac tacgcttaac ttctggcctt cattcaggca ggtcagcagc
    30001 gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc gggcttcagg
    30061 ttacggatca gtttttcatc cgccagatgc agcgttttgt acggcccgtc tttgaagagt
    30121 ggcgtatgga aagtcagaat atccgcatgc tgaactaatt catccagcga gcggaaatca
    30181 ccctcatccc cacggtcagc gcgaggcgga tcgcaaagca aggttttaat ccctaacgct
    30241 tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc caccgtacgg
    30301 tcgtgcagtg aaaatccatc gcgttcggca agcatcagta gtgaggagag aacatattcc
    30361 accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc cttctttaac
    30421 catgcttcat cgacatggtc tgtccccgca gtggcagtgc caacaaattt aatgggtttt
    30481 cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct
    30541 gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc caaacggcta
    30601 aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct
    30661 gtatgagacg agagttaacc ggacaagtgt gccataatct cgcggccagg catacttgcg
    30721 aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc ctcctgggca
    30781 accaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca
    30841 gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacagcaaag
    30901 agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct
    30961 ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa
    31021 aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc tggggaataa
    31081 tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct
    31141 ctcaccggag caattaaaaa ccattctcac cctgttgcag gaaggcaaga tggttattcc
    31201 gcaaccgcag cagcgcgagg cgaccgatcg tcctctatta ccggcggagc acaatgcgct
    31261 caaacagctg gtgaccaaac ttgcggcggc aacgggggag cccagcaaac agatctggca
    31321 atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa
    31381 ccatctggtg acctggctgc aggcgcgtca gacgctaagc cagcaaaata cgccgacgct
    31441 ggaatcacta caaatgacgc taaaacaacc tttagatgcc agtgaactgg cggcgttatc
    31501 ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt ctgcccaggc
    31561 cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc cgcgtgatat
    31621 gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg
    31681 tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggttcgtta
    31741 accccgacga aacgacagta tcgtgacaat aattcccagc accgcagaga tcgccccggc
    31801 aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac
    31861 gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga
    31921 ggggacacgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc
    31981 ggttaacgcc gcgcccgcta atgcgaccca cgcacccggg gcttgccaga gcagcaacaa
    32041 gcccaccgtt tctacaagca gagagacgat cgccactttc acgccgccaa aacggtccgg
    32101 catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa
    32161 gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc
    32221 aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc cgacaacgct
    32281 ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc
    32341 ccacgccagt aagggtaatg ccattgtggt gagcgccagt gcggcaaaac cgtaatggct
    32401 atgaatcaac aggccaagcg gtgcaccaac agcgagggca ccgtaaatcg ccattccgtt
    32461 ccatgacatt actttgccag agtgttttgg ccctacgatg cctaatcccc aggtcagagc
    32521 gcctgtcagt aactggcttt caccaaagcc aagaatcaaa cgcccgacga ccaacagggc
    32581 aaatttgaac ggtgcggaga cgggcaaaat cgccgccagc agcaacgcgc cccccgccag
    32641 accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat cggccagtcg
    32701 cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atgccgacgg caatgccgac
    32761 catggtattg ccatagccca attcatgatg aacaaacaac gggataaccg gcaacggcaa
    32821 ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg cggaagagcg aaaaattggc
    32881 agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa
    32941 ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggaat
    33001 taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct ggaacaggcg
    33061 gcgattgtca atgatgtgaa aaagggaacc atcgggttcc ctttggcgtt agtgccggat
    33121 gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt aacgtcggat gcgacgctgg
    33181 cgcgtcttat ccgacctact ccgactactc cggcctactg cgaattaatc tttcagcttg
    33241 cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacagtggtc
    33301 agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct
    33361 tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct
    33421 tcctgtacgc cagcagcgcc cagagagtga ccggtcatgg ctttggttgc agaaatcgcc
    33481 gggctcttat cgccgaacac ttcacggata gctgccagct ccttcacgtc gccaaccgga
    33541 gtcgaagtgc cgtgggagtt caggtaatcg attggggtat caacgccatg catcgccatc
    33601 tgcatacaac gtactgcgcc ttcgccagac ggagcaacca tgtctgcgcc atcagacgtt
    33661 gcgccgtagc caacgatttc agcatagatg tgagcaccac gcgccagcgc gtgttccagc
    33721 tcttcaacca ccaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcatcg
    33781 taagtacggg aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccattgcg
    33841 tcgaattcgc aagccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc
    33901 tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac
    33961 gcggagctga tggagtagtt aacgccatga attttaaacg gggtggcgag gcaggcagaa
    34021 acgccggatg ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag gccgcgcggg
    34081 ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg agccgccgcc ggaacctgca
    34141 atcaggccaa cgcgcgggtt attctggtaa gcttccggag agaggcccgc atcagcgatt
    34201 gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg
    34261 cggtcaatga ggccagtggt atccagtttt acgttgcccc agacgtggct acgcatgccg
    34321 gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg
    34381 acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt
    34441 ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca
    34501 cttgtacgcc gaacaagtcc gatcagccaa ttaatagaga aatttgcgca gccttacaca
    34561 catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac actactccat
    34621 acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga
    34681 tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc tcggaggtaa
    34741 ccaattagag gcacgctttc ctgagcatct acatcctctg tttgtggtag cagagagcgg
    34801 cttcggcacc ggattaaact tcctgacgct atggcaggca tttgatcagt ttcgcgaagc
    34861 gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac
    34921 ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca
    34981 acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattac ttgatgaagg
    35041 ccgcgtgacg ctggatttat ggtttggcga tattaatgaa ctgatcagcc aactggacga
    35101 ttctctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag cgaaaaaccc
    35161 ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac
    35221 gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac
    35281 gatgcaaaaa cgtaagggct ttggacgcaa acgggaaatg ctttgtgggg tgatggaaca
    35341 gacattacca ctcccctgct ccacgccgtg gtttaatcgt acgggcagca gcaaacggga
    35401 agtagcgatt atcggcggtg gtattgccag cgcattgttg tcgctggcgc tattgcgtcg
    35461 cggctggcaa gtaacgcttt attgcgcgga tgaagtcccc gcgctgggtg cttccggcaa
    35521 tcgccagggg gcgttgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt
    35581 ctctaatggg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca
    35641 tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca
    35701 gatgctgtca atggatttac ccgaagaact ggctgtagcc gttgaggcaa atgcggttga
    35761 acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag gtggttggct
    35821 gtgcccagca gaactgaccc gtaatgtgct gaaactggcg caacagcagg gtttgcagat
    35881 ttattatcaa tatcagttac aggatttatc ccgtaaggat gactgttggt tgttgaattt
    35941 tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc atcaaatcag
    36001 ccgtttcagc caaacatcat ctctgccagt atattcggtt gccggtcagg tcagccatat
    36061 tccgacgaca ccggaactgg caaaactgaa gcaggtgctg tgctatgacg gttatctcac
    36121 gccacaaaat ccggcgaatc agcatcattg tattggtgcc agttatcatc gcggcagcga
    36181 agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga ttgattgttt
    36241 cccacaggca cagtgggcaa aagaggttga tatcagtgag aaagaggcgc gctgcggtgt
    36301 gcgttgtgcc acccgcgatc atctgccaat ggtaggtaat gttcccgatt atgaggcaac
    36361 actcgtggaa tatgcgtcgc tggtggagca gaaagataag gcggtaagcg cgccggttta
    36421 tgacgatctc tttatgcttg atgctttggg ttcgcgtggt ttgtgttctg ccccactgtg
    36481 cgcagagatt ctggcggcac agatgagtga agaaccgatt ccgatggatg ccagtacgct
    36541 ggcggcgtta aatccgaatc ggttatgggt gcggaaattg ttgaagggta aagcagttaa
    36601 ggctgggtaa ccagctttgg cattgtttgt cggatgcggc gtgaacgcct tatccgacct
    36661 aagtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag
    36721 acgccgcaac ttactgctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca
    36781 gcgcctggtc acgcggcgac aattcaccag caccaatggc tttttccaga ctctggctaa
    36841 cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta
    36901 atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa
    36961 ccataccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc
    37021 attgtaaaac gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg
    37081 taagttccgg cgtgtgataa taattttgta gtgctttaat gaaacgtgcc gggcgttccg
    37141 gaatgccatt tgttagataa tccatcacct gcgcatgtac tcgccgttga aacaccacgc
    37201 ggtccggttc gaagtcgcct tccagattgt cgcaactgac gttaaacgga tatccagccg
    37261 ccacgcagaa caaccagtcg agcgcctgcg gcttcacttc aacatcttca aactggcttt
    37321 gcgtttgggc atcgcgtccg tccgggcaat accagtagcc gaaatcaacc agctcacggc
    37381 gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccat
    37441 gagcaaagac gattcggtta tacggtactt ccgcatcagc aggaagatag atcggttcgt
    37501 cgtcgccttt aatcagacgg gtattaaaat catcggcaaa gcagctatta aaaatttcaa
    37561 tcaactgctc atagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc
    37621 ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc gcggagacga taacaatcat
    37681 cgggcggatc agcttttgtc ctttgctcaa caccagtcgt gaacccatgc gcgcgccgag
    37741 gaactgcccc accagcatca caaagcccgt cgcccaaatc actttgccgc cgagaataaa
    37801 cagcagcaaa ccaccgatgt ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt
    37861 ggcgaggttg aatccgcaca gcgtaacaaa ggccagcgcg taaaacgatc cggctgccgg
    37921 accaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga agggtaaacc
    37981 gtacatccgg cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc
    38041 gatacaaatc accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac
    38101 cagcagcgcg ccgctcattg agccgacaaa ggtcatggcg atattgagtt tctgatcgct
    38161 taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc
    38221 ttgcagttta ttggttgcca gcgcattagc gggagacatc cccgccgcca tcaatgccgg
    38281 aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac
    38341 aaaaaagaga actcccagca acagcgggga aaccataaac aggctattaa acgtttccat
    38401 tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc
    38461 ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg
    38521 caaccatcgc ctggtggcgg taaaggttga tcttcacact ccagactatc ggcaggacaa
    38581 cgtaatcgta catgcatatg cgcacgatgc tggaaccagg gtcgcacttt gcgcaaccag
    38641 tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg
    38701 cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc cggcttccac
    38761 agcgtgggga caacgcgttt accgtcgcgg gatactaagt ccagtgcttg cgggcgcaag
    38821 agctgcgcgg aggtccagcg agttttcggc agttgcagaa agatatcgac atccagtccg
    38881 gtctggtggc tggcatgacc gccgttgaaa cgcccaccag cgggcatccc catatcgcca
    38941 atcagcaccg tacccatgcc cagattgctc acctggctac tcagacgctg gataaacatc
    39001 accaggtccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg
    39061 gactgtatcg gcagcgtgtc agcgccgacg atacagccat tagaaaaact gcctatcgat
    39121 tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctgacg
    39181 ctactggcaa gcagggccag tagcgcaatc gcggttttgt tcatttttta ccagcgtgga
    39241 atatcagtct tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaatacg
    39301 atcgccagca tcgcttctgc gatcggcacg gcgcggatcc cgacacaggg atcgtgacgg
    39361 cctttggtga tcatctcaac ttcttcgcca aagcggttaa tggtgcgccc cggcacggta
    39421 atgctggagg tcggtttcag cgccatatgg gcaatgattt gctgcccgct gctgataccg
    39481 ccaagaatgc cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc gtcgcgattc
    39541 tgactgccgc gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg
    39601 ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac cggctcacca
    39661 agcccggcag gaacgccact ggcaacaacg gtgactttcg cgccaataga gtcgccctct
    39721 tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtccgggtc cgggcagaag
    39781 aacggatttt gttcaacctg cgaccagtct ttgatttcca gcggaatgtc gcccatctgg
    39841 gtcaggcagc cgcggatttc aataccaaat ttctcggcga gatatttttt ggcaatcgcc
    39901 cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac gtccgccgcc gcgataatca
    39961 cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc ctgggcggaa aacgtcttta
    40021 attgcgctgt aatcctgaga acgctgatcg gtattttcga tcaacaaacc aatgctggtg
    40081 ccggtagtaa caccttcaaa aacaccggag agaattttga cctgatccgg ctcacgacgc
    40141 tgggtggtat agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg caggtccgct
    40201 tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc
    40261 gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct
    40321 ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttcacgggc
    40381 cgccagcagc tgctctttgg tgagcataaa cacaccatcg ccgccgttat caaactccag
    40441 ccaggtgaac ggaacatccg gatattgttc cataagatgt accatgctgt tgccgacttc
    40501 acaaatcagc acaccatcat cagcaaggta atctgccgcg ttaccgagaa tgcgacgcgt
    40561 cagtttcagg ccgtcagtgc cagatgccag gcccagttct ggctcgtgac ggtattcgtt
    40621 tggcaggtcg gacatatctt ccgcatcgac atacggcggg ttagtgacaa tcaggtcgta
    40681 ctgcactttc ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag
    40741 accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc
    40801 atcgacttcc gcttccggga aggcataagc acaggcaatg gcaatgcagc cgctaccggt
    40861 acacatatct aaaatatgct gcggttgctt gctgataagt ccggcaaatt tattgttgat
    40921 caattcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatgacc
    40981 gcagaaccag gctttattgg tcaggtaagc caccggaatg cgttcgttga cgcggcggat
    41041 cacgcgctca acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc
    41101 cggaatatcc agcggcaggt agagcgacgg caacaccaat tgtaccgctt catcccacgg
    41161 gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctaa ccgaccagcg
    41221 caacatgtcc tgaatggttt gcagctcatt aactgcttca tcaacgaaaa ttttatccac
    41281 gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga
    41341 taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgcgct
    41401 acgagtcggg taaactgtag gaaaattaga aatagagacg cataaatgaa aaagaaaaca
    41461 aca
//
LOCUS       DAZGBC010000020        41111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGBC010000020 DAZGBC010000000
VERSION     DAZGBC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.020"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(220..1134)
                     /gene="ydeQ"
                     /locus_tag="ACHK1J_001137"
     CDS             complement(220..1134)
                     /gene="ydeQ"
                     /locus_tag="ACHK1J_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910443.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENISLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(1194..1697)
                     /gene="ydeR"
                     /locus_tag="ACHK1J_001138"
     CDS             complement(1194..1697)
                     /gene="ydeR"
                     /locus_tag="ACHK1J_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910444.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(1710..2240)
                     /gene="ydeS"
                     /locus_tag="ACHK1J_001139"
     CDS             complement(1710..2240)
                     /gene="ydeS"
                     /locus_tag="ACHK1J_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910445.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(2254..4905)
                     /locus_tag="ACHK1J_001140"
     CDS             complement(2254..4905)
                     /locus_tag="ACHK1J_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7910446.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSECTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTNGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDIYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLRLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            complement(4947..5657)
                     /locus_tag="ACHK1J_001141"
     CDS             complement(4947..5657)
                     /locus_tag="ACHK1J_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7910447.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKDEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(6018..6335)
                     /locus_tag="ACHK1J_001142"
                     /pseudo
     CDS             complement(6018..6335)
                     /locus_tag="ACHK1J_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(6340..6537)
                     /locus_tag="ACHK1J_001143"
                     /pseudo
     CDS             complement(6340..6537)
                     /locus_tag="ACHK1J_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6808..8130)
                     /gene="hipA"
                     /locus_tag="ACHK1J_001144"
     CDS             complement(6808..8130)
                     /gene="hipA"
                     /locus_tag="ACHK1J_001144"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO7910448.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            complement(8130..8396)
                     /gene="hipB"
                     /locus_tag="ACHK1J_001145"
     CDS             complement(8130..8396)
                     /gene="hipB"
                     /locus_tag="ACHK1J_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO7910449.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(8607..14027)
                     /locus_tag="ACHK1J_001146"
     CDS             complement(8607..14027)
                     /locus_tag="ACHK1J_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO7910450.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNGQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGGNEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATILEGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLINSKDYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSSEGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSDVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDIENTTSVNVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGVSDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDNHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGV
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGSLTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSVSPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDLTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMSGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSRLSQGSASSMWMRHVGGHERFRTGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDSNKTGAYVDCWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHNRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            complement(14558..16150)
                     /gene="lsrK"
                     /locus_tag="ACHK1J_001147"
     CDS             complement(14558..16150)
                     /gene="lsrK"
                     /locus_tag="ACHK1J_001147"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO7910451.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(16229..17182)
                     /gene="lsrR"
                     /locus_tag="ACHK1J_001148"
     CDS             complement(16229..17182)
                     /gene="lsrR"
                     /locus_tag="ACHK1J_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO7910452.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            17431..18966
                     /gene="lsrA"
                     /locus_tag="ACHK1J_001149"
     CDS             17431..18966
                     /gene="lsrA"
                     /locus_tag="ACHK1J_001149"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO7910453.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAVGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRFSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            18960..19988
                     /gene="lsrC"
                     /locus_tag="ACHK1J_001150"
     CDS             18960..19988
                     /gene="lsrC"
                     /locus_tag="ACHK1J_001150"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO7910454.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKTAS
                     SGKKREAA"
     gene            19988..20980
                     /gene="lsrD"
                     /locus_tag="ACHK1J_001151"
     CDS             19988..20980
                     /gene="lsrD"
                     /locus_tag="ACHK1J_001151"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO7910455.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLGLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            20992..22014
                     /gene="lsrB"
                     /locus_tag="ACHK1J_001152"
     CDS             20992..22014
                     /gene="lsrB"
                     /locus_tag="ACHK1J_001152"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO7910456.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            22041..22916
                     /gene="lsrF"
                     /locus_tag="ACHK1J_001153"
     CDS             22041..22916
                     /gene="lsrF"
                     /locus_tag="ACHK1J_001153"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO7910457.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            22940..23230
                     /gene="lsrG"
                     /locus_tag="ACHK1J_001154"
     CDS             22940..23230
                     /gene="lsrG"
                     /locus_tag="ACHK1J_001154"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO7910458.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            23287..24045
                     /gene="tam"
                     /locus_tag="ACHK1J_001155"
     CDS             23287..24045
                     /gene="tam"
                     /locus_tag="ACHK1J_001155"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO7910459.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(24049..24963)
                     /gene="yneE"
                     /locus_tag="ACHK1J_001156"
     CDS             complement(24049..24963)
                     /gene="yneE"
                     /locus_tag="ACHK1J_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO7910460.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(25160..26611)
                     /gene="uxaB"
                     /locus_tag="ACHK1J_001157"
     CDS             complement(25160..26611)
                     /gene="uxaB"
                     /locus_tag="ACHK1J_001157"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO7910461.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(26838..28256)
                     /locus_tag="ACHK1J_001158"
     CDS             complement(26838..28256)
                     /locus_tag="ACHK1J_001158"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7910462.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(28395..28754)
                     /gene="yneG"
                     /locus_tag="ACHK1J_001159"
     CDS             complement(28395..28754)
                     /gene="yneG"
                     /locus_tag="ACHK1J_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO7910463.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(28754..29680)
                     /gene="glsB"
                     /locus_tag="ACHK1J_001160"
     CDS             complement(28754..29680)
                     /gene="glsB"
                     /locus_tag="ACHK1J_001160"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO7910464.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(29744..31132)
                     /gene="sad"
                     /locus_tag="ACHK1J_001161"
     CDS             complement(29744..31132)
                     /gene="sad"
                     /locus_tag="ACHK1J_001161"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO7910465.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIAAAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            31233..32114
                     /gene="yneJ"
                     /locus_tag="ACHK1J_001162"
     CDS             31233..32114
                     /gene="yneJ"
                     /locus_tag="ACHK1J_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7910466.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            32192..33307
                     /gene="yneK"
                     /locus_tag="ACHK1J_001163"
     CDS             32192..33307
                     /gene="yneK"
                     /locus_tag="ACHK1J_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO7910467.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPARNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     SPHFQDLFLFFASMLLHYHQFMNPNESDLNNVLMPASLSDDKIIKHMARKTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDIPDRH"
     gene            33458..34648
                     /gene="ydeA"
                     /locus_tag="ACHK1J_001164"
     CDS             33458..34648
                     /gene="ydeA"
                     /locus_tag="ACHK1J_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO7910468.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(34673..35338)
                     /gene="marC"
                     /locus_tag="ACHK1J_001165"
     CDS             complement(34673..35338)
                     /gene="marC"
                     /locus_tag="ACHK1J_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO7910469.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            35550..35984
                     /gene="marR"
                     /locus_tag="ACHK1J_001166"
     CDS             35550..35984
                     /gene="marR"
                     /locus_tag="ACHK1J_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO7910470.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            36005..36388
                     /gene="marA"
                     /locus_tag="ACHK1J_001167"
     CDS             36005..36388
                     /gene="marA"
                     /locus_tag="ACHK1J_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO7910471.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            36420..36638
                     /gene="marB"
                     /locus_tag="ACHK1J_001168"
     CDS             36420..36638
                     /gene="marB"
                     /locus_tag="ACHK1J_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO7910472.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(36669..37568)
                     /gene="eamA"
                     /locus_tag="ACHK1J_001169"
     CDS             complement(36669..37568)
                     /gene="eamA"
                     /locus_tag="ACHK1J_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO7910473.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGALLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            37763..38950
                     /gene="ydeE"
                     /locus_tag="ACHK1J_001170"
     CDS             37763..38950
                     /gene="ydeE"
                     /locus_tag="ACHK1J_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO7910474.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVDSVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGLVLTNLPPSSLFIILALVIIAAWVLMLKGIRARPWRQP
                     ALC"
     gene            39077..39172
                     /gene="mgtS"
                     /locus_tag="ACHK1J_001171"
     CDS             39077..39172
                     /gene="mgtS"
                     /locus_tag="ACHK1J_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO7910475.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            39172..39277
                     /gene="mgtT"
                     /locus_tag="ACHK1J_001172"
                     /pseudo
     CDS             39172..39277
                     /gene="mgtT"
                     /locus_tag="ACHK1J_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
     gene            complement(39392..40282)
                     /gene="dgcZ"
                     /locus_tag="ACHK1J_001173"
     CDS             complement(39392..40282)
                     /gene="dgcZ"
                     /locus_tag="ACHK1J_001173"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO7910476.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FAAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(40536..40928)
                     /gene="ydeI"
                     /locus_tag="ACHK1J_001174"
     CDS             complement(40536..40928)
                     /gene="ydeI"
                     /locus_tag="ACHK1J_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO7910477.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT        10527 a        10274 c         9494 g        10816 t
ORIGIN      
        1 ctatttttaa tctattatcg ttaaacaata gattaaatac gataaggtaa gcatcatctc
       61 tcatagtgtt taaattaaca tattttttaa tataaaaaat aagactctgg cttcaattgt
      121 gcgcggattt tcttacaggt gtaggataat agccttcaca aaataattat ccgcgcaatt
      181 ttcgtgaatg gagcgcatag accaatgtca aaatcacaat tattcataga taaaagtgac
      241 accaatgact gactggacgg ttccggctga aaccttgttt cctgtttgtc cataggttgc
      301 gcttaaaccc agaggtactt tgcttttatt tactgtcccc agggagatat tttctcccgt
      361 agccagtatt tttccattac gcatcagaga gacacccact cccgatgctt ttgttgcatc
      421 aggtgctgtg ttagcaaaca cttgtcgggc actgtcagtg gtggcacccg aaagataaaa
      481 tgagagcttt tgttcgcttg agcaatatac gccaaggggg atttctgcgc ttccaggaaa
      541 gtccgggaga ttcacggtga cattacgact atcaacagtg cagccgcctg ttggcataac
      601 aacactgtta tttgaaataa tattccacgt aaaattacgc ggatttccgc ttcctaacgt
      661 ggcaatttta tacatatgaa tacgggcaat aacttccccg gcttttatga caactccacc
      721 ggccgccccg actggggtaa tatagagttt taacggtaat ggcatcggtg tcttatcacc
      781 aatatccaat acgttggtat ttgtcgtcaa cgggaagggg taggtcacat tattccagta
      841 cagcgatcct ttatatgact gtagagagcc tgcgaaagct gagccttgta ccaggtttat
      901 atgatcagtg tcgtaccagc cgccgtaatc attccagcaa cttatatgct gagacaagtc
      961 tacaaccaga ttctggcccg gctgtattac agggtcgaga ttaacataaa cagaggttgt
     1021 accagctcca atacttgacc cgccatcgac attacaactg aaggcaaaaa ctttgcctgc
     1081 cattaataga agataaatac cgaacaggac ctttatactg attgttttac ccatgatata
     1141 tcctaaggtt aaaaaattga tttaaaaaga aggctaagaa aatcgaatcg acgttattgc
     1201 caggtgtaga tcacattgat tagcgcctcg atcgttccct ggcttgcgtt tccattcacc
     1261 gtgatagctc ttgccttaag tggaaactgt gcattacgag tgatctcatc aacaataacc
     1321 gttttgctat cgccattttt taacgtcgca tcctggtcat ccctaagctc tatctgaata
     1381 ttttcggcag taccttcatt tttgtaataa cccgtattgt cagttgaacc tgtcacgatt
     1441 gccgtcactg cacttgtttc agccggacaa tcggttaatg acaaagtaat attgtgccag
     1501 ccagatgcag aaccaggttg ttgcagattg cgcgtataaa gatccccgag attaacgtta
     1561 gcttctttgg tttgaatagt gcagggttta gcgactaccc gaccattaac agtgatagtg
     1621 acatcggcgg cctggagagt tgctgtgaat aacgcgcaaa acgtagctaa caggaacctc
     1681 ttgtgaagtc ttttcatatc tgactctgat tattgaaatt caagggtaaa ggttgccgaa
     1741 gccctcacca gtcccggatt gacggacttc tgagttgact tcagacgagc ggagtaaggc
     1801 aaaatattgt tctgttctgg taccagtggg atccactgca tcccggcatg aaggtcattc
     1861 agtttcaccg gacgcatatt tccgtccagt atttctatac ctaaaccgga ggccgtattg
     1921 cttccctcat ccagtttcaa cagagtatta ttttctgcat cctcaatacc gttaaatgca
     1981 acccgaaccc ccgttgtccc tttgctgcat tcacttaacg taatctgaaa agggacggct
     2041 ggacttgtgc taccggtcgt tggaaattgt ctggcactgt ttttttggag atctacggta
     2101 aaattaagcg aatccgatga gactgtgcag ccataatcga ggacgcgccc gctaatttta
     2161 ataacgctat ctgcggataa agcagaatag gtggttaacc ccagacataa accgaggaaa
     2221 ataacgttat tgtatttcat aatctattgt tccttagcga cagattgctg tctgctggtt
     2281 cagcaaggtt ccaggagaga cttcaggaag cttgtaatcg acaatacagt ttgagttttt
     2341 atcattgccc catgaaacct gtaatttccc tgactgtgga agtccagtca gataaacctg
     2401 accattttcc gcgacaatgc tgccattttt attctctccg tgagtgacaa ttgcaccgaa
     2461 tggaacgctt ttattaccgt acttcaacgt cattaatact ttcccgccga tttgtgcatt
     2521 aaatgttgct ctggcaatag caccgtgagt tgggatgaca gtgactacgg tttcatccag
     2581 ttcaacatta tctgcaaggg aattcgcgtt aagagcgaca cgattttctc tatattctgt
     2641 cgcaaatggt aatattgcat agccacgcca gtcggtatga attccggtct ggttctctat
     2701 tttgacatta tcagcgccag gagccttaac cagaaccatt gtgtcgccca gcggctgtcc
     2761 aaaggtgatg ccatcagcat gagcaataat cccaccactc attccgtaat agatctggct
     2821 gctgtcacca ctccgactgt aaccgacatt cgtattaccg taagctccac gataattaag
     2881 agaactgtaa ccactggtgc cagacgatgt attacctccg tgggtgttac cgacctgaac
     2941 gctataattc aggttattat ccggcagcag agtgccataa acccccgata gattggtcat
     3001 gccgcctttc aaatcgtttg acatactgta actggcgttt gaattacgaa atgccgactg
     3061 actgtctgta cgcatccaat gactgaaggg aacattaagc gtaaaagcga gtaaatgatc
     3121 ccgatcgttt tgccatatat tattggaata gctgtaattc agtgaagtcg taatatcacc
     3181 aaacggcaca tttaatccaa atgatatttg ctggtcgctg cgtgacgtgt tccagtaact
     3241 ttggcgactg gcactgaaaa atgtcgtacc gtaatttcca agctgctgag agatgcttat
     3301 ttgttcctga ccacgcttac tgtagaacag attaaaataa tcaataaatt gtgtctgctc
     3361 attgctgtct ccggtaggag gcttgacggt gtaaccactc attcgactgt aggcactgtc
     3421 ggataagtta taaaaacctt gcgtagaata gcgatatcct gcgacctgga tattggttcc
     3481 tgtctggtag aaggatttgc tgtagacgga tttaaccgat tgcccgctgt gacgggtgcc
     3541 atctgcaagt gtcgtattgg cctgcgtgat atcgaaagaa aaggcaccaa aaagtcttaa
     3601 atctttacca ataccaaggt tgaaggcctg ataatcttct gcaatttgca ttccgccata
     3661 aggtgtccag tttccttcca gtccatgcat caagctacct tgtatgaacc tgggggagct
     3721 ttgcaggtta tttccactac gatattcccc catggcaagc gcataacgcg tatatccagc
     3781 acgttggaga accggaacag acgaataagg aacatataat gtctgaatac tgccgtctgc
     3841 ctcttttatg gttacttgca gatcaccacc attggccgca gagttgatat catcaatagt
     3901 aaaagggccg ggtggaacag tagtctgata aatatcgtat ccgttctgtt ttacactcac
     3961 ttgtgcggtg ccacgggcaa taccatgaat caccggagcg aaaccatgtt ggctatcggg
     4021 caacatcgct tctgttgaat ttattttgag gccacgaaag ttcacactat caaaaatatc
     4081 gccattggta taactatcac ctaccgttaa ccgtgagcgt aagggaataa tgtcacgttc
     4141 agccgacgta ttgatatgct gccatttatt gctgtcagat gaattgctac tcccactgtt
     4201 ataactccac gttgagttat cgcgcagtcg ccaactacca atgttgatgc cactctgtaa
     4261 attcaaatat gcatagttgg attttcctgc attatggtta ctacgattat taatagaatt
     4321 accattaaaa ctatagttta gaagccctgc atttattcct tcttcccaca gatcaggaga
     4381 aacatagccg cgagccattc tgccaacata aatttgcgga acggagagtg acaaatgttg
     4441 ctgaccaaca tcaaactcag ttgatgcgtc agccaaacga tcctgaagtg gtgtgcattc
     4501 gctgttgtcc ggcacatgtt tttctgccga atgttcctta ttgtccaata acgcagattt
     4561 ttttatgcca agactgacaa gaagatctgt actcaggcat gggatgagat ctgcgttatt
     4621 atcatctgca ataaaagtaa tatcccgact agtcataaat tcgtcgttca aataaatgtc
     4681 gacccgatat gtaccaggtg gtgcctcgcg tcccttagta aaggcggata aatcgacaga
     4741 ttcagcaaga tcattgctta aaaaccgtgg attaaaataa tactctgcgt caacgggaaa
     4801 taccgcagtt gctaagctaa gggcaatggt aagccgtgca aagcgaggca gtagtaacac
     4861 ctgatgcata cgatatgcct taaatgcggc acgaaaggct gtcattatta attccgattg
     4921 agtttattat taattaggtc actggcttac tgcatggttg ccttaatacg cggagttaat
     4981 gcaccgtaat cattgatcgt ctgaaaactg atatccccgg tggcagcatg cgggatatcc
     5041 accgatactt catcttttgg cggcaccatg gtgtttggga gattgctatt tcctgctttc
     5101 atatttgtta cggtaatgaa gtagggcgtt ggattaataa gagtgagttt gctaccgctg
     5161 cgacgaaacc gaagcattgc aggcgcttct tcaggtgcca ttgccaggtt cgtggggcga
     5221 taaaacattt ttatgcggct aataattgcc agctgtaatg tattctcatt cttctggtct
     5281 ttttccatag caggaatagc tttaacatta acccagaaca ggctttcacg gtcgcctggt
     5341 aactgatgat tagtggcgtt gattatccgc agcgtattct cttttttacc ttgcatggaa
     5401 aaaagcggtg gggtaatgac aaactgggtg tctttttggt cacctgcatt ttctatccat
     5461 gactgaatga gatagacact ggcaggatca ttattcgtga caggcaataa aacctgtttc
     5521 tggtttgccg gataaattac ccgagtcgca cccagtgcga cagctgcgtt ggcaacggtg
     5581 ctatggcaag caaagagcaa taaaagtaac actgtcgcca taaaggaaat agagtagggg
     5641 gtacgcgtag tttgcatctg atgcattctc catgtgttaa gaggataagc gggaaaaccg
     5701 ttacatcaga caatcggtaa atgttgctaa tacattggcg agtcgccggg tttccagact
     5761 taatgcgttt gtaataaatg ttggttctct tttaatagga aaaccttgct acaaagtaaa
     5821 tattttaaat aacagtaagt ttaactactg ttgtttattg atagtttaga tattatgtga
     5881 tttttcatag cgctccaaat acaaaccact cctcattaat aatgagattc acattggtga
     5941 attgctcggg tgtgcgtagc catgccacac acaccctgtt tcaatttcag atgacgtata
     6001 gccaccttaa gattgaatta tagatattga actttgaagg tcgcatctgc gtttgcagta
     6061 ccagctacgg actgcccgag tgcaatataa cgagcctgga acggcagtat attcgtgcca
     6121 tcaatcaagt cagttttagc gctaaaagtg gcaccatcaa gtattaatac ctcacccgta
     6181 cggtccaaaa tttggatacc gacattttgg gcagagcctg ccgctgaact ctgcagcgct
     6241 aacgtatcgt cattactggt gacagtagta cccaggaatg caacagctgc attactggaa
     6301 acattggtat cacaatcatt cagcttgata ttgaagctat tcccggctga aatgggataa
     6361 gctttcgtaa ccgacagcat aggctgctgt ggtgacatcg taatgttcat tcagcatcgt
     6421 tcgcctgact tcattgagac gtagccattt ctgataccgg agtggactca ttcccgcaag
     6481 ctgcctgaag tgttgatgga aagagggcgt gctcatttgc acgcggttta ccagcttgag
     6541 tcttcaacgc tgggaagatg cggcaaacgg caaatctgca taccagccag cctatatccg
     6601 gcatgcacag gccaattatg aaagaggaga gactcaagtt ctggaaccct gaacgaataa
     6661 cgactaccgg aaaatcactt cgtatcactc agacacgatt taacataata tacattatgc
     6721 gcaccaacat aaaccaagga agaatctagt cgttggcggt catgattgtc ctgctcatta
     6781 acaacgacca aaccccatat cttactctta cttaataccg tattctcggc ttaaccgacc
     6841 gtgcaacctc aacacattgg tttcaactgc cgtcaccacg ttctctggaa aatctgtcgg
     6901 taatgaagtc ttcacgttat ccagtgctgc cggaatcatt ctggcaaagt cactcaggat
     6961 ttcatgcatc tgcacttccg ggaatctcag cacctttgct gtcgccaaaa aatgtcgcgg
     7021 ataaatttta tcgattgccg tttttttgcc tttggatgcg ttaagcccca ttgccagttt
     7081 gagatcgctg atgtgtattc ccgcgccacc caataccgga aatgctgaaa tgatgtcgta
     7141 aaatggcgtg agtcgataac tgccgccagc ctgaataaat acggagaagt tttttgcatg
     7201 gccatccgtt gcgccaatca accactgaaa gacctggaat ttcataaaat catagcgatc
     7261 tttcagcgcc tcgctggacc ccatcaaaaa agccatgatc cgcgcgatgc ctgggcctcc
     7321 atctgattca tatttcaccg atgaaggtaa accgaatgtc tgacacatat cctcctgtgg
     7381 caagcgaagt aaaaccgttc gctcagcatt ccaacgcctg tcaaaacgtt cgaccgctaa
     7441 cgcgcgcaca tttcccgctt taatgatttc tgcgtccgga acattcaacc caagttcttt
     7501 cgccagcagc agacagtaat actcattatc aacgctttgg ctgagatcga gcgtcgcatt
     7561 gggctgcctg atttccccaa tcggtaattt aatgatgtgc gtcgtcggcg ttattccttt
     7621 cggaatgcac cagtcattgc ctattctgag cagtgctgtc ttctcctgtg cgccagcaac
     7681 cgagatgcga aagtcatttt cttctctaat catgcctagc gggatatctg ctttataagc
     7741 cgttaatact tcttcaagtc tggcttcagt aagcttttcc catgccatta tcgggcgcgt
     7801 tatggtttca ttttcgggta ataacgttac ggcaccaacg ctgtctcgcc ctatttctga
     7861 caataaatca aacggttgtc tggatttagc atgataacgt ttaacgatcc ggtcacgtac
     7921 aatcgggcta tcgggtaaca agttatcgaa gaagttaaat acggcatcag aggtgatatt
     7981 ccccctctgc aatggcagcg aaagtgacaa cggtctggca taacggctgg ctaaccactc
     8041 cggtgcatac ttaaaggtgt gcgcgccgtt ggctaacttc gttaactcgc ctacccgctg
     8101 gttgttcatc caagtgacta gtttgggcat taccactcca gatcttgctg ttctgttgat
     8161 tctggcgagg catttttcgt gtcgcatagc gtcattgaga gttcaagcga ctgtaaaatc
     8221 ttaaaaaatg tcgtgagcga ggtattgtca gggttgtttt cgaaattgga aatcgtcgcc
     8281 tgcttaatgc cgattttttt cgccagctcg ctctgcgtcc agccattttg ctggcgaacc
     8341 agtttcattg cattcgctaa ttgcgttggg ctatagatct tctgaaagct catcatgtca
     8401 tacgtccaca gcaaatttat ccgcttaagg ggatattatc agttttatcc tttagtgagg
     8461 ataaagtcaa tctggaaaca catcttatcc gctgtacggg ataaacgctg cgatatccgc
     8521 gatcgcggat aaaagggagg ctttaaaagc cttttgaagt tggcaatatt aagccgcatg
     8581 ccatctcgac atgcggctta tactgtttac cagctatatt tcactcccag catgccctga
     8641 gtatcgctat agcctttatc acctagttgc acaccgacat tcccccacag gctaaggttg
     8701 ttatttactt tcgcctcaac cccggtacgt acttcaccga gattacgcgc accatcacgg
     8761 cttacggttt gaccattcat cttcacggcg tagactttgc tattgttgat ccagttcgct
     8821 tcaatgtaag gctggaactc acgctgttta ccatcgtcac gctggtgatg gctattcagg
     8881 taggttttca ccccaagtcg cgtttgcacg tttccgtcgc cttccgtttc aatgcgtgtt
     8941 ccgtctttcc ggttatggtc agaatctttc acacccatcc aggtgatttg cgcctgtggc
     9001 tggacgtacc aggtattcag cgtcccttcg ctgccgctaa atgttcccgc ttcaaaggta
     9061 tacccaccct caacagaggc cgtaacaccg cgagaatcat agtcgtcagc agaacggtta
     9121 tcggaactga cgctgttatc aaaccagtta tacagcgccc agcagtcaac ataagcgccg
     9181 gtcttattcg aatcgttctg ataccaggtc gcgtacagcc cagcgctgta accgctgatg
     9241 cgcccatccg atttataacc cacacgatta ctctgagtat tactgtgctg attggcgtag
     9301 cctgccatca cgccaagatg ccagcgatcc tgcgcgttgc tactccactg cgccaaatcg
     9361 ccgcctagct gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg
     9421 aaacgttcgt gtcccccgac atgacgcatc cacatactgc ttgccgaacc ctgagaaagc
     9481 cgtgaatctg tatactgcgg ctcacccaga cggtcgtgta agcgatggct aaacagcgag
     9541 ttggctgcgg caatgttgct gatatagctt ccggcctccg ggcgataaac tgatgggcct
     9601 tccggatcca caacaggggg attattgatg ggatcgggtg tatccgctgg cgttacgccg
     9661 tcccatttac tggtcagata ccagtttttc tcgtcattcc ccttcccttt agccagcgtg
     9721 tagacgtaag ccccagcttc gacagttccg gtggtcaacg cgaagttacc tgcagaatta
     9781 ccgccaacct caatgagttc aataccgttg accgtttgcg caccgacgcc gccaatgtta
     9841 tcaacccgaa cgcgagtgtt cccttgggta tcgcctttca cgttcatctt atcggtgggt
     9901 gaattatcgc cgcccagcgt cgcgttgaac gtcatcaggc cattgtttcc ggtatagttg
     9961 ccgttaacgg tcagtacatt ccccggtttt cctccttcac tgttcatttg aaccgtgccg
    10021 ccattctcca ggttgccgcc gattgtggtt ttagcgacac gcagtgtccc cccagacata
    10081 acgttcacat cacctgcagt ggtgacattt cccgacatca caccgctgtt atggatatta
    10141 atgtgctgac ttgccatatt aatggcagag tcggaaccga aagcaatttc accacccacg
    10201 atatccgtcg taccctgata cgccgcggaa tcgttgttaa gagtcaggat cccggtaccc
    10261 agttttgtca aatcaccgct accgctgata ctgtgcgtca tcgcccagct acgctcagtg
    10321 gagatggtga gcaggccatt attgataacg gaagcatcac ccaggttttt ctgctcgttg
    10381 acgctgagcg cgctgcctgt atcaatgttg aattgcccca caaagcggct attatcgcca
    10441 tccagctcta catctgtcct ggcggttgcg ctcacgatac ccttcccgct tatgctatta
    10501 cgcagttcgc cagtcacgtt ttttagcgtt aacagaccgt cattggcaat atttcctctg
    10561 cctaacccct gcgtattatc gaggctgact tccgcatttg ggctaacgct ggttaacgca
    10621 ttgtagcgtg cattaagccc ctcaatagcc agcgttcccc ctgtaacatt caggtttccg
    10681 ccacctgtca gttcaccctg actgatccca cctttattta ccgtcagcga cccttctttg
    10741 aacaaaacag tcgaacccat ctgcccggtg aatgtctcta ccgtctgcgt cgatccatta
    10801 agatcgacga tggctgcgtt gctgatgttc agttcccggg tgttgcccag cgcgccatcc
    10861 gcatcggtac gtaatgtccc catctgaacg taggttgccc cctgatagtc gttctgaccg
    10921 ttggaaagcg aaacctgtcg caccgtattg attgccagat ccccttcacc accgattttt
    10981 gccgacatat cggccgttgc gccataggct ccgccatgtt cggctaacgt cagcttttgc
    11041 cctccatgga tgttcagcgc tttcagccca tagttaacgt acaaaccatc acctggtgcg
    11101 gtcgtgaggc ggactccata atcaccctcg gcgaccaccg ttccgttttg cgcaatgtgt
    11161 aacgttttgt ccgcttccac ctcgtcgccc tgtaaatcac gtaacgttaa tgagccaccc
    11221 gacccaataa ccgtttgcgc cttcaccagt tgaacgccga catggttatc atcgtgttcc
    11281 agcaaattga gcgtcgtcag agggttattc gccataggat cattccacgg tttaggcacg
    11341 tcgataagca cgtcgcccgt cccgtttacc tgatagttac ggcctttcca ggtgtagtca
    11401 ctcacgccga caaccagcgt atcgacgctg atatatccct ctgcaagcgt cgcagcagga
    11461 atatccgtat cgaaaatgag cgtaccgcca ttcatggcca gtccaccaat ggattgctct
    11521 cccacgttta ccgatgtggt attttcaatg tcagactgca acatcgcgtg agtaagcgca
    11581 gcggtgttgt cgcgttccag agtgaaggta ctgtctttca gttgggcaac accggcgaat
    11641 tcagtccctg ttgcatgggt aaagccaaac atcttgtcgg aggatgacag ctgaactcgc
    11701 atcaagccat cgcctttgag cgcattggtc agcgtgtaat ctcctgcact gttcgttgat
    11761 gcgagaatgt cgagcgtacc ggggcttgca atggttacat cactcgaacc catgcccgtt
    11821 accccatctg ccgtatgaga cgcaatggtc aggaggccgt cattgaccag cgttttaccg
    11881 ctataagtat tccctgcatt gaacaccgta tggcctgcat catggatcac ttttccgtct
    11941 ttatcgtcac ctgtaatcag catatcgacc tggtagccgg catcactgtt attggtatga
    12001 ttaaagacaa aaacaccttc gccagagcca aactctactt ttgtcgcgtt ggttatatac
    12061 ccggcatctg ccgccgcctc accgtgagct gcaccaatgt ttagcgttcc tacagcaggt
    12121 tcaaaaacac ctaagtaaac ttctccacct tcgacattca gcgtgccgct attcgtcagg
    12181 gttaacgtac ccgtaccaga tgtccctacg tacatattga atgtttcaag aagagagttc
    12241 tgcccatcca ccctcacgtc gcccttactt ttgtcattga gagctatctg tgtatcacgc
    12301 gctttaacta tacctccggt ggtaatattc agttcacccg tgcctaatac accgacctgt
    12361 agcaattggg cgttagtaga agacgtcttt aaattccaga gactatccgt tgaaatatta
    12421 actattccct taccggaggc atttcctccg ataaatccat agcccgttga attaagggtg
    12481 gcaccattac tgacatttac gataccgcta ccaaaattac cgacattgat acccacagta
    12541 tcgaacgatg atgtcgcatc catcaggttc agtgtgccga taccgcttgc tttatcgccc
    12601 aaaaaagtaa tattttgcga tacaaactta ccgccatcgc taatattgag gtttccgaca
    12661 cccgcaacac caatggtggt atagacattc ttattgacct cccacatccc ccccgtggtg
    12721 atgctgacat tcccgacgcc ggtttctcca taaccgagtg aacttcctcc gttgctgaca
    12781 acaagcccct cattactgat attcatttcg ccgtggccgt cataaccaag attagttccg
    12841 agattagtga taacggagtt cttatcctta acagtaatgt tgcctgtgcc tgtttctttc
    12901 atccccgcag tgataattcc cgaatctact ttgccttcac tcgagacatt aagttcaccg
    12961 tcgccagcat caccgatata gatatagcgg aaagcttcgc ctgttccgag gaaattccaa
    13021 tgccctttat cggtcacgtt gacgacaccg tgggaaccgt cctgaacgcc caccaatgaa
    13081 taatctttgc tattaattag tccattattg gaaatattga ctgcgccatt accgaaatat
    13141 ccattataga gtctgcgtac cgtgatgaca gagtcttgat cctgcacatt cagggttccg
    13201 acaccggtgg cattgccacc gataatcgta ttttcagccg taatcagccc accctcgaga
    13261 atagtcgcct ccccagttcc ttgattacca atttgaaatt caattgagga atcattattt
    13321 tttattagcc actcgccacc cttttcaacg acaaccttac cattactgtt cgcttgatat
    13381 cctaaaatgg cgactattga actcgtgacg taacccttat ccgtaatatt taatgagccc
    13441 gtaccgtaac tccctatttc gaataattcg gtcgtcagaa ccgagtcctc tccctcaaca
    13501 ttgaccgtcc cgacgcctcc tgtcgaggaa cctagtctta aatagcctcc atcaacgtga
    13561 cctttctgtt taatattcag cgtccccgtt ccggattgac ccacatttaa aggccttgca
    13621 ttatttccgc tatcatacaa tcgccaggtg ccacccaaaa cattaacggt gccctctgag
    13681 tcctcattac cgccaatgac gctggtagta atagtggtta aggaggcatt accgccagcc
    13741 aaaatattaa gcactccagt gccataccag ccaaccaggt aggcatcata agcaacatca
    13801 gtatcaatat tggtaatctg accattatca acttccagac tcgcaccaga cgctgaaccg
    13861 cttagtgcca gcaaaacacc cagcgtcaat ctactgaatt tcgttgtcag tccagaggat
    13921 ttacgcaaat taaccgtcga tgttttacct gccctgcgag ttaattccga gcaggcctga
    13981 aatacctgta gagtgcaatt ccatatcacg cgatagattc tattcatata aacgctccat
    14041 atacaaacaa tacagccacg aaagagatga gtcgtcagac attaaaaatg aaacttatta
    14101 aattgtcaga ggtctgtatt gagtgttagt tggaggtgcg aaggtgtcaa tttggctaat
    14161 ttgctaatgc tttcgaattg atagattaaa aattgcatag ttcttatttt atttaaaaat
    14221 gaactatgag ttattgttgc ttaataagaa aactgtttct ttcaatagga aaattatcat
    14281 catcatattt aacaaagaat agcactaatt gctaaaaatc gaagtttatt aaaccccttt
    14341 tgcatccatt aatgtcttta ggaatattcg ctataaaata agggttatgc ttaatgcttt
    14401 aagaaaaata gcaactttcc cttgaatata gtactggtga tggaacgatg aatctgcaag
    14461 ctggcattta acaagccagc tctaaaagaa gggaaataag aaaaactata ctcaaaaact
    14521 aacagccacg gtcatcatga tgtggctgtc aatgaaacta taacccaggc gctttccata
    14581 acgatgtcgt cagtccatga tcaaccagcc ccagctgatc ctgataaact gcctgccact
    14641 tatcgcgtga atcctgataa agttcatgct tttccgggtc tggtgtgtgc gtccgttccc
    14701 agcgaaccag gcgttctccg gtttctgcca ttgatgaaaa aattccggca ccgacgccag
    14761 ctgcaatggc acatcctaat gcggtggctt ctttgaccac cggaatattg acgggtaatc
    14821 ccgagacatc agcgagaatt tgactccata atttcccttt tgaacctccg cctgcaaaga
    14881 ctaacgatga aggatgaata ttcgagaaat cagcaatttg ctgcaagtta cacgctgata
    14941 caatcgccgc attttcttcc agcgcacgga acaatgtcgc tttgttacat ttatccgggt
    15001 caatggacaa gttaataaag gaaggcgcag cgtgatacca ggttttaaag cgcattctgt
    15061 cggagaagat cggcattacg ccccacgacc caggcggcac ccgactggcc atctcttcca
    15121 gcagcgtata ggtgtcgatg cctaaacgtt cggcaatcag tttttcttcg gcacagaaag
    15181 catcgcggaa ccagcgcatg gtgagtccgg taaaaaagct tatagattca gcttgtacca
    15241 tgccaggaat aacatgagga ttaacgcgca cgttcatttc tgggtctgtc accggcgcgg
    15301 ctaaatttac aacttgctgc cagaatgtgc cgccaagaac cgcggtttgt gccggacgca
    15361 caacgcctaa ccccaggcaa ccaagctgca cgtcgcctcc tccaacgacc accggagtgc
    15421 ccaccttcag accgcagagt tccgccgctt gtgaacttac cacgcccagc aatgtgccgg
    15481 tttctttgac aggagaaaga atatcggcac gcaggccagc catatccagc aatgcaggtt
    15541 tccagtcacg gatggttaga tcaagaagtc ccgtggtgcc agcgttagag ggatccaccg
    15601 ccagttcgcc gctgagcata taggccagcc agtcgctgat catggtgatg gttgatgcct
    15661 gacggtaaat atcggaacga tggtgcgcca gccaaagtaa tctggggatg gcacttaaag
    15721 ccagtgtttg tccggtcgcg cgataaactt cgttttcaaa ggtattgttg tgcagttctt
    15781 taagttcgct aacttcgcgc gctgctctgg catccacatt ggcgcaggcc cagatcggtg
    15841 ttccttcatt gttatataaa acaatgcctt cacgcataga acatgccgaa acggcagcga
    15901 tatactccgg gtctatgccg gcgttgtgca gcgcctggcg catacactca cacgccagtt
    15961 gccagttttt gttgagatca aattccatag aaccaggaac gtccggtact gccagatgcc
    16021 gccactccgc ctgtcccact gctatttgat tgccttccag gtcgaatatc acagcccgaa
    16081 tacttccggt gcctgcatcc agcgccatca ggtagtactt tgattctgaa ggggtaaaga
    16141 gtcgagccat tagattatcc tcggcttatg cgttctgtct gttcctctat acgttctcca
    16201 tcattcccgg taataaggtc atgcaaattt aactacgtaa aatcgccgct gctgtgtcct
    16261 gatcggtaac cagtgcgttg atataaccgc ctttcattgc agcggcaatt gcttcggctt
    16321 tattttctcc ccctgccacg ccaacccgga cgggtatggt cttcagcgcg cttaaaggta
    16381 agccaatcag ttcgttatgt attttgatat ccgtgacaac gtcacctttt gcatcaaaaa
    16441 agtagcctaa aatgtcgcca accgcccctt ttcggccaat cattaactgt tcgccctggc
    16501 tgatataacc ggagcgaatg atcgtcgcgt cgtcttgttg actcacggcc ccaatgccga
    16561 caatcgccac atccgctgct tgcgcggcta acagaacatc tttgacgcaa ttttcatttt
    16621 ttagcgtacg ggcaatgtca gcggaggatg cccgcaacgg agccggaata atattcacac
    16681 tgcacgccgc gttaagctgc ccgatacccg tcatatagga accgacgcca ccggagagcg
    16741 tgaccaggcg aatttgctgt gacgaaataa aaccacttaa gcgttgcagc gtattcatgg
    16801 ttgcctcgcc aaaaccaatc gccagcatct gttgtggttg aagtaaactc atcaacatat
    16861 gcgccgcgcc tatccccagt cgcccgccga catcagcatc cgcaagccca gggatcaccc
    16921 ggacatgttg cagcgaaaac tgacgacgta attgtgtttc atattccaga cagccttcaa
    16981 agcgagaatt aatctgcacg cgaatgatgc cggactgatg ccctttctcc agtaatcgcg
    17041 acactttcaa acgggtcagg ccgagacgat cgctgatctc actctgggtc agcccgtcgt
    17101 ggtagtaaaa ccacgcgatc cgcgcgacct gttcttcttc acacattccc tgttctgaaa
    17161 ttgccgaatc gttgattgtc ataatttatt cttcactttg aacatattta aatctttaat
    17221 gcaattgttc agttcttgcg catttatatc tgtgatggca accacagttt gactctacga
    17281 gcatgaacaa acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta ttcgtcggaa
    17341 atatgtgcaa tgtccaccta aggttatgaa caaattaaaa gcagaaatac atttgttcaa
    17401 aactcacctg caaaactgaa cgggggaaat atgcaaacga gtgatacccg cgcgttaccg
    17461 ctactttgcg cccgctcggt ttataaacag tattcagggg ttaatgtcct gaaaggcatc
    17521 gattttacgt tgcaccaggg ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa
    17581 tcgacgttaa tgaagattat tgccggtatt acccctgctg atagcggtac gctggagatt
    17641 gggggcaaca actacgtcag attaacgcca gttcatgctc atcagctggg tatttatctc
    17701 gttccccagg aaccgctgct tttcccaagc ctgtcgataa aagaaaacat cctgtttggg
    17761 ctggcaaaaa aacagctctc catgcagaaa atgaagaact tgctggcggc gctgggctgc
    17821 cagtttgatc tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa
    17881 atcctccgcg ggctgatgcg cgactcgcgg attctgatcc tcgatgaacc taccgcctcg
    17941 cttacccctg cggaaaccga acgcttgttt agtcgcttgc aagagctgct tgctactggc
    18001 gtgggtattg tttttatctc gcataagctg ccggaaattc gccagattgc cgatcgaatt
    18061 agcgtgatgc gcgacggaac catcgcctta agcggcaaaa ccagcgaact gtctaccgac
    18121 gacattattc aggccatcac gccagtggta cgggaaaaat cgctctctgc cagccaaaaa
    18181 ttatggctgg agttacctgg taaccgccca caacatgccg taggaacgtc ggtgctgaca
    18241 ctggaaaatc tgactggcga aggttttagg aatgtcagcc tgacgctaaa tgctggagaa
    18301 attctgggtc tggctgggct ggtgggtgcc ggacgcacag aactggccga gacgctctat
    18361 ggtctacgta ctttgcgtgg cggacgcatt atgctgaatg ataaagagat caatagattc
    18421 tccactggag aacgtttact gcgcggtctc gtttatctgc cggaagatcg ccagtcctcc
    18481 ggactgaatc tcgatgcttc gctggcctgg aacgtctgcg cccttactca taaccttcgt
    18541 ggattctggg cgaaaaccgc gaaagataat gccaccctgg aacgatatcg tcgggcgctg
    18601 aatattaaat ttaaccaacc ggaacaagct gcacggacat tatccggtgg caaccagcaa
    18661 aaaatcctga ttgccaaatg cctggaagcc tcgccgcaag tattgatagt cgatgagccg
    18721 acgcgcggcg tggatgtctc ggcccgtaat gatatctacc agctgttacg cagcatcgcc
    18781 gcacaaaatg tggctgtgct gcttatctcc tccgacctgg aagagatcga actgatggca
    18841 gatcgtgtgt atgtgatgca tcagggcgaa attacccact ctgcactgac cgggcgcgat
    18901 attaatgtcg agactattat gcgcgttgcc ttcggcgata gtcagcgtca ggaggcgtca
    18961 tgctgaagtt tattcagaac aaccgtgaaa tcacggcact gctggcggtg gtgctgctgt
    19021 ttgtattacc cggttttctc gaccgccagt atttaagtgt gcaaacgctg accatggttt
    19081 atagcagcgc gcaaatcctg atcctgctgg caatgggcgc gacgctggta atgcttacgc
    19141 gcaatattga tgtttcagtg ggttcgatta ccggaatgtg cgcggtgctg ttggggatgt
    19201 tactgaacgc ggggtattca ctacctgttg cttgtgtcgc gactttactg cttggtttgc
    19261 tcgcaggatt tttcaacggt gtcctggtcg cgtggctaaa aatccctgcc attgttgcca
    19321 cccttggcac gttagggttg tacagaggca tcatgttgct gtggactggc ggtaaatgga
    19381 ttgaggggtt acccgccgaa ctgaaacagc tctccgcccc gctgctgttt ggcgtttccg
    19441 cgattggttg gttgacgata attctggtgg catttatggc ctggctgttg gcaaagacgg
    19501 cgtttggacg cagtttttat gccacgggcg ataatttaca gggcgctcgt caactgggcg
    19561 ttcgtacgga agccattcgc attgtggcat tttcgttgaa cggctgcatg gcggcactgg
    19621 cgggaattgt gtttgcttcg cagattggtt ttatccccaa ccagaccggt accgggctgg
    19681 aaatgaaagc aattgcagcc tgcgtgctgg gcggtattag tttgctcggc ggttccggtg
    19741 cgatcatcgg tgcggtactc ggcgcatggt tcctgacgca gatcgatagc gtactggtgc
    19801 tgttgcgcat tccggcatgg tggaatgatt ttatcgcggg tctggttctg ctggcggtgc
    19861 tggtgtttga tggacgcctg cgttgtgcgc tggaacgtaa tctgcggcgg caaaaatatg
    19921 cccgctttat gacgccaccg ccatccgtta aaactgcttc gtcaggtaaa aaacgggagg
    19981 ctgcataatg cgtattcgct acggttggga actggctctt gccgcactgc tcgttattga
    20041 gattgtctca tttggtgcaa ttaacccgcg gatgttagat ctcaatatgt tgctgttcag
    20101 caccagtgac tttatctgca ttggcattgt cgccctaccg ctgacgatgg tgattgtcag
    20161 tggcgggatc gatatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
    20221 actgtttcaa agtggtgtgc cgatgccgct ggcgattctc ctgggcttac tgctcggcgc
    20281 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
    20341 tacgcttggc acgctgtatc tgtttgccgg aagtgccctg ctgctttccg ggatggccgg
    20401 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
    20461 ggatgtgctt ggactccccg ttccgctgat tatctttctg atatgtctcc tcgttttctg
    20521 gctctggctg cataaaaccc atgccggacg taacgtgttt ttgattgggc aaagcccgcg
    20581 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
    20641 ggggctggcg tctgcggtcg ccgctgtgct gctggtgtcg tattttggtt cagcacgttc
    20701 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
    20761 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
    20821 tttgcaacaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
    20881 tgcgctactt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
    20941 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
    21001 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
    21061 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
    21121 agcggtggca acggcgcaca acaagcgggt aaagagctgg gcgttgatgt gacctacgac
    21181 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcag
    21241 ggttataacg ccattatcgt ttctgcggtt tcgcctgatg gcttgtgtcc ggcactgaaa
    21301 cgcgccatgc aacgtggtgt gagagtgctg acctgggact ctgatactaa accggagtgc
    21361 cgctcttact acattaatca gggaacgccc gcccagttgg gaggtatgtt ggtggatatg
    21421 gcggcgcgtc aggtgaataa agacaaagcc aaagtcgcgt ttttctactc aagccccacc
    21481 gttacggacc aaaaccagtg ggtgaaagaa gcgaaagcga aaatcgccaa agagcatcct
    21541 ggctgggaaa ttgtcactac gcagtttggc tataacgatg ccactaaatc attacaaacc
    21601 gcagaaggaa tattaaaagc gtatagcgat ctcgacgcca ttatcgcccc cgatgccaac
    21661 gccctgcccg ctgccgcaca agccgcagaa aacctgaaaa atgacaaagt tgcgattgtc
    21721 ggattcagta cgccaaacgt gatgcgtcca tatgtggaac gcggcacggt gaaagaattt
    21781 ggcctgtggg atgtggttca gcaaggcaaa atttcagttt atgtcgcgga tgcattattg
    21841 aaaaaaggat caatgaaaac gggcgacaag ctggatattc agggcgtagg tcaggtggaa
    21901 gtctcgccaa acagcgtcca gggctatgac tacgaagcgg atggtaatgg catcgtactg
    21961 ttaccggagc gcgtgatatt caacaaagag aatatcggca aatacgattt ctgatgtgca
    22021 ttacttaacc ggagtaagtt atggcagatt tagacgatat taaagatggt aaagattttc
    22081 gtaccgatca accgcaacaa aatatccctt ttaccctgaa aggttgcggt gcgctggatt
    22141 ggggaatgca gtcacgctta tcgcggatat ttaatccgaa aacgggtaaa accgtgatgc
    22201 tggcttttga ccatggttat tttcagggac cgactaccgg acttgaacgc attgatataa
    22261 atatcgcccc gctgtttgaa catgccgatg tattaatgtg tacgcgcggt attttgcgca
    22321 gcgtagttcc cccggcgacc aataagccgg tggtactgcg ggcgtcaggt gcgaactcta
    22381 tcctggcgga attaagcaat gaagccgtgg cgttatcgat ggatgacgcc gtgcgcctga
    22441 acagttgcgc ggtggcggcg caggtttata tcggcagcga atatgaacat cagtcgatca
    22501 aaaatattat tcagctggtt gatgccggaa tgaaagtggg aatgccgacc atggccgtga
    22561 ctggcgtggg caaagatatg gtgcgcgatc agcgttattt ctcgctcgcg actcgaatcg
    22621 ccgctgaaat gggggcgcaa attatcaaaa cctattatgt cgaaaaaggt tttgaacgga
    22681 ttgttgccgg atgtccggta cccattgtta ttgctggcgg taaaaaatta ccggagcgcg
    22741 aggcgctgga aatgtgctgg caggctatcg atcagggcgc ttctggtgtg gatatggggc
    22801 gtaatatttt ccagtctgac catccggtgg cgatgatgaa agccgtacag gcggtggttc
    22861 accataacga aacggctgat cgggcatatg aactctatct gagtgaaaaa cagtaactgc
    22921 ggatttaagg agaagaatta tgcacgtcac actggttgaa attaacgttc atgaagacaa
    22981 ggttgacgag tttatcgaag tttttcgcca gaaccacctg ggctctgtac aggaagaagg
    23041 caatttgcgc ttcgatgtct tacaggaccc ggaagtgaat tcgcgctttt atatctacga
    23101 agcctataaa gatgaagacg cagtggcgtt ccataaaacc acgccccact acaaaacctg
    23161 tgtcgcgaaa ctggaatctt taatgaccgg gccgcgtaaa aaacgtctgt tcaatgggtt
    23221 gatgccgtga ggcgaattta tcagttttat ctacaattgg ggtaacgcgc tgacgggagt
    23281 aaataaatgt ctgactggaa cccctcttta tatctgcact ttgccgctga acgatcgcgt
    23341 ccggcggtgg agctgcttgc cagagtgccg ctggagaata tcgaatatgt tgccgatctt
    23401 ggctgtggcc caggtaacag caccgccctg ctgcatcaac gttggcctgc tgccaggata
    23461 acgggcatcg actcgtctcc ggcgatgatt gctgaagcgc gcagtgcttt gccagactgc
    23521 cagttcgtgg aagcagacat ccgcaactgg caaccggaac aggcactcga tctgattttt
    23581 gctaatgcct cactgcaatg gctgcccgac cactacgaat tgtttcctca cttggtttct
    23641 ttacttaatc cgcagggcgt gctggcagta cagatgccag ataactggct ggagccgacc
    23701 catgtgctca tgcgcgaagt tgcctgggaa caaaactacc cggatcgcgg gcgtgagccg
    23761 ttggctggcg ttcatgctta ctacgatatt ttgagcgaag ccggatgtga ggtcgatatc
    23821 tggcgaacca cttactatca ccagatgccg tcgcaccagg cgattatcga ttgggtaaca
    23881 gccactggat tacgtccgtg gttacaggat ctgaccgaga gcgaacagca gctttttctt
    23941 acgcgctacc atcagatgtt ggaagagcag tatccactgc aagagaacgg acagatactg
    24001 ctggcatttc cgcgtctgtt tattgttgcc cgccgtacgg agtaaatatc acgtcagttg
    24061 gtaatggcga tcgggaagaa tttttgctgg aatttcggct tcatcgttca tctgtaagag
    24121 gtcaatttca atagcgttgc agatggcatc cagcggtaaa tcattgtttt cagtaccgaa
    24181 cggatcttcc agttcttccg ccagacaatc cagcgaaata aaagtatagg aaatcagcac
    24241 agagataaaa ggcgtcatgt aatgcaggtc cacgaccagc gcgaacggca gcatgataca
    24301 aaacagataa acggtacgat gcaaaatcag ggtgtaggca aagggaattg gcgtgtaggc
    24361 aatgcgctcg cagcctgcca gaaccgctga aatatcgtta agccgatcgt tgaggctaat
    24421 aaacaggata tctgaaagtt gtccattgcg tcgctgaacc gccaaccact cccccattat
    24481 taacaagatg cggttagctg gagagtttga agccagtaca cgctgaagat cttcagtctt
    24541 gagataatga gccagcactt ccgcctgtgg ctgtttgcgt aatgtcatgc gtaaacagtg
    24601 ggcgaaggcg atttgcagcc gggcaaactc ccttacactt gccgaatctg gcaatgtcgt
    24661 ttttacctcg cgcagtaacg accgtgaggc aatcatcaac tgcccccaaa gttttcgcgc
    24721 ttcaacgtaa cgggcgtaac cggcattatt acgaaaacca agaaaaatgg cgatggcgac
    24781 accgagaatg ctgaacggtg cgagggtgaa tttgatgccc agatgcgtgt accagggcag
    24841 catgaaaata acagcgatag aaaagagaaa attgagtagt aagcgcgagg atatcttgga
    24901 taatactgag ccatgccaga caaaaatacg gcgcagccag tgttgttgtg gacgaacaat
    24961 catggttatc ttcaggcgtg gaaaagtcgc cctattaaac gtgattacga tcacattctc
    25021 aagacacgac ttacaaatta cctacaagct tacaacatta acaggcgctg catgtggcac
    25081 ctgttaatga taaaggtata taggatgttg taactaatat agtaaaataa gaaacccggt
    25141 cgaaaccggg ttcagagtat tagcacaacg ggcgtactgc ttcgcgcatc cctttttcga
    25201 gaatcgcatc caggtcatta gccacctgct cgaccagacc aggcacttgc gtcaggtcct
    25261 gctcccagtg gtctttttct gccaacacaa tcgctaccag ttcctgggta ccgataacac
    25321 ggtcacgatg ctgactccag agttgctgat aacgttccag ccagtgagca tcgtcctgta
    25381 ccggatatgt ttctccgtta cgctcaccac gatagaacgc aatcaatgcc gctaatgcga
    25441 aagtaaggcg cgccggaagt gtgccgtttg ccttctgccc tgccagtagc tgcggcagga
    25501 tgcgggtgcg gaacttggtc ataccgttga gcgcgataga cagcagctga tgcttaatgt
    25561 acgggttacg gaaacgcccg gtgactgcac tggcgaaaga ctccagttca tcgcgaggca
    25621 aatccagaac cggaataatt tcttcgtaga tggctttttc aacgaatgcg caaatttcag
    25681 catcgttcat cgcttcacct acggtatcca gccctgcctg gaacgccacc ggcaccaacg
    25741 cggtgtgtgc accgttgagt attgccactt tgcgttcttt atacggttta atatcgtcaa
    25801 caatcagcac gttgagcgga tatttgtcca gacgcagctc ggtcgctaag gatttcggtc
    25861 cctgaatcac aaacaggtaa aagtgttcag cggtgtcgag gaaaccatcg tgataaccca
    25921 gttcttcttc cagtttagct acttcatcac gcggataacc ggtaacgata cggtccacca
    25981 gcgtagaaca gaagctgtta gcctgatcca gccattgaat aaatgcttct ggcagtgccc
    26041 actcttgcgc atagcgcagc accagttcac gcaacgcgtc gccattgtag tcgatcaact
    26101 cacacgggat gatgatccaa cctttatcca gcgcaccgtt gaaatggcta aaacgttcga
    26161 acagcagacg ggtcagtttt gccggataac ttaccgctgg cgcgtcatcg aatttatcgc
    26221 ccgcatggta gctgataccc gcttcggtgg tgttagagaa aacaaagcgc atttccgggt
    26281 tgtgcgccag tttcaggaat tcatcgtatt cactgtagac gctgatttca cgattaacag
    26341 agcgaatcag acgcgcgtcg ctgaccgctt cccccttctc attcaggcca cggataatgg
    26401 tggtgtacag accatcctgc gtgctcagtg acggcgggaa tgaagtttca atcggacgaa
    26461 caacgaccac accagaattc agatcggtgt gctcattcag gagatcgatt tgccagtcaa
    26521 caaaggcgcg caggaagtta ccttcaccaa actgaatgat acgttctgga tactgtgcac
    26581 cgggaaaatc gcgacgattt agtgttttca caatgggttc ccttctgatt agtcatacaa
    26641 cctgtttgaa ttggtacgac aggttagcaa actttaatac gccgaacccc tgttttgatc
    26701 aactcctgat gattaatgag cagttttatg agaaaagtgt ggcgcggatc atggtttaat
    26761 cgaggaaaaa cgccttttcc tggatcataa agtggtagaa cacattgcat ttaaatcgct
    26821 ctgaatgaac aaagatgtca gacaacttcc tcaccgtaac gcatagtgct ggtacggttg
    26881 cgcccatctt tcttcgagcg atacagacac gtatcggctt cgaccatcaa tttattaaag
    26941 tcatcggtca gcgttcggta cgatgcgcga ccactaccga cgccaatact taccgtgaga
    27001 taaagcgttt tttgttgcca ggtgaatggt tgcagttcaa cgcctttacg gattttttcc
    27061 gccattagca gaccatcgac aggattcacc gacggcactg caacagcaaa ttcttcgccg
    27121 cccatgcgcg ccaccagccc cttatcaccg acaatcttct gaatatgccg ggcaaacacg
    27181 cttaacactt tatcgccaca ttcatgtcca tagttatcgt taatactttt gaaatagtcg
    27241 atatcaagca gcatgacagt cagatgttgt gtctgtttca gcgatggact tttcagcgcc
    27301 tcataaagac cggaccgtga gtaaacctga gtcagaaaat caaagtcggc tcgcagcgca
    27361 acttgcttca ttagcgaatt gatcgctgcc acgctaaaag aaaccataat tgggcatatc
    27421 gccatcgtgg caataccgag acgtgcggag aacatttccg gaatggagaa cggcgaaccg
    27481 accgagatat caatcaccga attagccacc agtacgattt ccaccgcacc ggtgacaaag
    27541 gtcaacagac atgttacctg cggcgtatag cgcactgcac accagattaa tgcaggcagc
    27601 gggaacgcca gactccccgc tccgccaatg actaccgagg caataaccga cacaataagc
    27661 gcgatggcgg gcatcatctg ttctgcttta aagcgcggca gcactccagg aatagccaac
    27721 gtcagcatac aaggcacgat caacacgccc gttgagaatt gctcactgaa ccagtccgca
    27781 agcaaaggcc agaaatccag actatcaata ctgaccgaac caatcgcccc gacaatagcg
    27841 cataataatg cggcaatcag acagtaatta aatagccgta aggcacttac tggctcatat
    27901 ttatttttcc ccaaacgctt atcccgcacg accagtaagg cgacggtaac aataaacatc
    27961 atattggaga aattgatagc cagtgaaacc agtccccatt cggtggttat ggcatcataa
    28021 accagcatcg ccacataact gatcgcatag taatgcagtc gattaagcca gacatagcgg
    28081 gcaaacaccc ccgccattac gccattcagc ggccagaaaa gagaaagttc gtgaaccagc
    28141 cgtaactctg caccaataaa atagaacagc gtggttagcg caaaaatagc gatgctatta
    28201 cgtagcagat gaccttcctg gaacacacgg aaagttgaaa tggggtgtac atgcattaat
    28261 tatctgtcca tacaattgcg ggtggcaatt atctcaacgt aataagtaaa ttctcataaa
    28321 ggatagcatg ttcgctgaca gcggcggcaa ataaaggtac cctgggaatg ctattttgtc
    28381 gttttccgca attctcatgg ctgattcatc tgtacaacca gccagtgata gattaccgcc
    28441 acgatataac gttgttgttc ttcgcttagc gaaacaccct gcggaatgtt atgccactta
    28501 gccagacaac cgcgacagca ggtcgcggtg gcgtgctgag cgataaacac cggatgcccg
    28561 cgcatggggg tttgcttacc gtcattagcc ggtaatgccg gagcaagacg tttagcgaca
    28621 aaatccgccg catgttgttc gatgactggc gcgcctttct ccagacaata ctgacgctct
    28681 ttcatgccca aacgaaagcg agatcgaaat tttgaacggg ataaacgcgc gaagagtgga
    28741 tcgagagact gcattaataa accgaacgcc ctaactgttt cgtcaattgt tcaaggacgg
    28801 cgatacccgc aagcgagtta cctgcatcat ccagttccgg actccagact gcgatggcca
    28861 tttcatgcgg aacaatcgcc acaataccgc caccaacgcc agatttcgcc ggtagtccca
    28921 cccgccaggc aaactccccc gcgttctggt acataccact ggtcgccatc agcgcgttaa
    28981 tttgccgtgc ctgcattggc gtcaccacag gttcatcaat atgaatagct ttcccctgat
    29041 tagccagaaa gacaaacgtc cgggccagct ctacacagct cattttcaga gcgcagtaat
    29101 gaaagtagtt ttgcagaacg gttgtcacgt catgatggaa attgccaaac gacttcatca
    29161 gccaggcgat agccgcattt cgcgcggaat gttcaaattc ggaacgcgct accaccgtat
    29221 cgtaggaaat atcagacaca ccgcttaagc cgcgcacgac ttccagcata cgttgccgtg
    29281 gtgcgcttaa tcgcccttgc aacatatcgc agaccaccag cgcaccggca ttaataaacg
    29341 gattacgcgg aataccctgc tccatttcca gttgcactaa agaattgaac ggtgatccag
    29401 acggatcttt gccgacgcgt tgccagattt cctcttcgga gtaatgacgc atggcgacaa
    29461 cgagactcag cactttggaa atagactgaa tggaaaaacg ttcttgcgcg tctccggcct
    29521 gaaaaagctg tccgtcaacg gtacagatag caatccccaa tcgggaaccg tctactgtag
    29581 ccagcgccgg aatataatcc gcgactttac cctgaccaat gagcggccgc acttgccgca
    29641 agatgttttc taaaattgca ttatccatgg cgactgccac tttctactcc tggaccgcag
    29701 gtctgaaaag acctgcgagt atatcagagc tgaatatgtc gcgtcagatc cggtctttcc
    29761 acaccgtctg gatattacag aattcgtgta agccgaaatg ggaaagctca cgaccaaagc
    29821 cactcttttt cacgccacca aaggccactc gcgcatcgct ggcacaataa ccattgataa
    29881 acaccccacc gcattccaga cgtgccgcca tctgtctggc ctgtgtttcg tcagtggtaa
    29941 aaatggtcgc tgaaaggccg aactcactat cattagccag ttccagtgca tgttctgcat
    30001 ctttcgcaat ggtgattgcc gcaacggggc caaacatttc ttcccgaaac gcggtcattt
    30061 ctggggtaac attcgccaga accgttggcg gatagtagtt acctgcccca gccatctttt
    30121 ccccgcccag taacaaacgc gcaccctgcg ccagggtttt ctccacctga tgatgcagct
    30181 catcacgtaa atcgaaacga gccattggtc cgagagcgtt ctcttcgtca cggggatcgc
    30241 ccattttcaa ggctgccgca gctgccacaa aacgttcggt aaatgccgca gcaattccct
    30301 cttcgataat aaagcgtttt gccgctgcac aaacctgtcc ggtattctga taacgtccgg
    30361 ctaccgccgc tttaaccgcc agttccagat cggcatcgtt aagcacaata aatggatccg
    30421 aaccgcccag ttccagtacg cattttttca gtgccgctcc agcctgtgca ccaatagccg
    30481 ctccagcacg aacacttccg gtcaccgtga tagcagcaat gcgcgagtct ttaatcatct
    30541 gagtgacacc gtcattgtcg gcattcagcc agccatatac gccttgtggg attcccgcat
    30601 ctttaaacac ctgggcaatg agctgagcac agcccatcac attcggcgca tgtttaagta
    30661 agtaaccgtt accagcaaga atgatgggaa cagcgccacg catcacctgc cataacggaa
    30721 aattccacgg cataatcgcc agaatcgtcc ccaacggtcg atactcaata actgcctgct
    30781 gattttccac cagcgtaggt tccgccttca gcattgccgg accatgttct gcataccagt
    30841 cacacaaatt cgccgatttc gccacttcag cgcgcgcctg gttgattggt ttgcccattt
    30901 cgcgggtgat catttgcgcc atttcttcgc tacgggcgcg cagagcctta ccgatatcac
    30961 gcagtttttc agcacgataa tctatatttg tctcgcgcca gtcacgaaag cctgctgccg
    31021 ccagctgaag tgcgttttcg atatcatcag cgccagccca cggcaacaca gaaagttgtt
    31081 cacccgtggc aggatttatc gaaattgcat gagttgccgg agtaatggtc atcggggtat
    31141 ctcctttatg agtcatggta tgaagatacg cagatttact cttgctttaa aatgaataat
    31201 attaagccac ttattcacga atcgagaata ttatggatct gacccaactg gagatgttca
    31261 acgcggttgc cgaagctggc agcattacgc aggctgcagc aatagtgcat cgcgtgccgt
    31321 cgaatctcac tacccgtcta cgccagctgg aaacagaact gggggtagat ctgtttattc
    31381 gcgagaatca gcgtttacgt ctatctcctg ccgggcataa ctttttacgc tatagccaac
    31441 aaattctcgc gttagtggat gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt
    31501 tattttctct gggttcgctg gaaagcaccg cagcagttcg cattccggcc acgctggcgg
    31561 aattcaaccg tcgttatccc aaaattcagt tttcactttc caccggccct tccggcacta
    31621 tgctggaagg tgtgctggag ggaaaactta atgcggcgtt tattgatgga cccattaacc
    31681 atactgccat tgacgggata ccggtatacc gcgaggagct gatgatcgtc acgccacaag
    31741 gtcatgcgcc agtaatccgt gccagtcagg ttaatggcag taacatttat gccttccgcg
    31801 ccaattgttc gtatcgtcgc cacttcgaga gctggtttca tgctgacggt gccgctccgg
    31861 gaactatcca tgagatggag tcttatcacg gaatgttggc ctgtgtggtc gcaggagcag
    31921 gtattgcgct tattccgcgc tctatgctgg aaagtatgcc ggggcatcac caggttgaag
    31981 cgtggccgtt agctgagcaa tggcgttggc taacaacctg gctggtctgg cgtcgtggtg
    32041 cgaaaacgcg tccgctcgag gcatttattc aactactgga tgtgcctgac tcgacaaaac
    32101 agggttacca atgagctatt tttgatagtt cttgcggcta atatgctcta tatagtgatg
    32161 ttccgatgac ttatgactat atggggcaaa tatggttacg ccagtaagca tcagcaatta
    32221 catatctctt cctgatgatt ttcccgttcg taatattgca ccgcaggtaa aagaagtttt
    32281 aaaggatttt attgatgcac ttagtacaat aatatgtaat gaagagtggc gcacgtcttt
    32341 aaacatcaac tcagccacaa aaaagatatt taataacctt gacaacttat catatattca
    32401 gaggacttct tttcggggta acgacacgct atacaatgaa aaggttcagt ttaaactcac
    32461 ttatcccgcc aggaatggaa gacacaaaga aaatattgaa tttcaggtag taataaattt
    32521 aagccctatt tatttagata atttccgcca tgatggagaa attaatattt tttgcgctcc
    32581 caacccaaag cctgtcacta tggggcgcgt atttcagacc ggcgtcgagc gtgtgctctt
    32641 tctgtttctg aatgatttta ttgaacaatt tccaatgata aaccctggcg ttcccatcaa
    32701 aagagcgcat acaccacata ttgaacccct gcctccggat caccataccg ctgcagatta
    32761 cttacgccag tttgatttgc ttgtcctgaa ttttatctct cgcggtaatt ttgttatact
    32821 cccccgatta tggaataact ctgaggttca cagatggttc gtcaataaag atcctaactt
    32881 gatcaccgcc attctcgaca taacggacag cgaattaaaa gaggatttat tgcaaagcct
    32941 gatggattca ttaggttcta acaaacatgt actacccgaa gtctgcatct gctttttatc
    33001 ccttttagca gaacaagaat ctcctcattt tcaggacttg tttttatttt tcgccagtat
    33061 gttattgcac tatcaccaat ttatgaatcc caatgaaagt gatttgaata acgtgttgat
    33121 gccagcatca ttaagtgatg ataaaattat caaacatatg gcgcgcaaga ccctcaaact
    33181 ttttgtaaaa aatgaaacac cgccaaaagt tactcatgaa gacctggtga aaaacaggcc
    33241 tcgctcccct gtcagaccac ctatacccgc aacctccaaa acgccagaca ttcctgatcg
    33301 tcattaaacc gtgatgttta ccgtctctct gacgcgtgaa agaatcagcg tcagagaaac
    33361 ggaaaacgcg atccagatca caaatgcatt gtattcacat cattaaccgt tttaagatca
    33421 tttcatcact ttttcgcaac tcacccgata atctgttatg acaacaaaca ctgtttcccg
    33481 caaagtggcg tggctacggg tcgttacgct ggcagtcgcc gccttcatct tcaacaccac
    33541 cgaatttgtc cctgttggcc tactctctga cattgcgcaa agttttcaca tgcaaaccgc
    33601 tcaggtcggc attatgttga ccatttacgc atgggtagta gcgctaatgt cattgccttt
    33661 tatgctaatg accagccagg ttgaacggcg caaattactg atctgcctgt ttgtggtgtt
    33721 tattgccagc cacgtactgt cgtttttatc gtggagcttt accgttctgg tgatcagtcg
    33781 cattggtgtg gcttttgcac atgcgatttt ctggtcaatt acggcgtccc tggcgatccg
    33841 tatggctccg gccgggaagc gagcacaggc attgagttta attgccacag gtacagcact
    33901 ggcaatggta ttaggattac ctctcgggcg cattgtgggg cagtatttcg gttggcgaat
    33961 gaccttcttc gcgattggta tcggggcgct tgttaccctt ttgtgcctga ttaagttact
    34021 tcccttactg cccagtgagc attccggttc attgaaaagc ctcccgctat tgttccgccg
    34081 cccggcattg atgagcattt atttgttaac tgtggtggtt gtcaccgccc attacacggc
    34141 atacagctat attgagcctt ttgtgcaaaa cattgcggga ttcagcgcca actttgccac
    34201 ggcattactg ttattactcg gtggtgcggg cattattggc agcgtgattt tcgggaaact
    34261 gggtaatcag tatgcgtctg cgttggtgag tacggcgatt gcgctgttgc tggtgtgtct
    34321 ggcactgctg ctacctgcgg cgaacagtga aatacacctc ggggtgctga gtattttctg
    34381 ggggatcgcg atgatgatca tcgggcttgg tatgcaggtt aaagtgctgg cgctggcacc
    34441 agatgccacc gacgtcgcga tggcgctatt ctccgggata tttaatattg gaatcggggc
    34501 gggtgcgttg gtaggtaatc aggtgagtct gcactggtca atgtcgatga ttggttatgt
    34561 gggcgcggtg cctgcttttg ccgcgttaat atggtcaatc attatatttc gccgctggcc
    34621 agtgacactc gaagaacaga cgcaatagtt gaaaggccca ttcgggcctt ttttaatggt
    34681 acgttttaat gatttccagg atgccgttaa taataaactg cacacccata cataccagca
    34741 ggaaccccat caggcgggag atcgcttcaa tgccaccctt gcccaccagc cgcataattg
    34801 cgccggagct gcgtaggctt ccccacaaaa taaccgccac caggaaaaag atcagcggcg
    34861 gcgcaaccat cagtacccaa tcagcgaagg ttgaactctg acgcactgtg gacgccgagc
    34921 taataatcat cgcaatggtt cccggaccgg cggtacttgg cattgccagt ggtacaaaag
    34981 caatattggc gctgggttca tcttccagct cttccgactt gcttttcgct tccggtgaat
    35041 caatcgcttt ctgttgcgga aagagcatcc gaaaaccgat aaacgcgacg attaagccgc
    35101 ctgcaattcg cagaccggga atcgaaatgc caaatgtatc catcaccagt tgcccggcgt
    35161 aatacgccac catcatgatg gcaaagacgt acaccgaggc catcaacgac tgacgattac
    35221 gttcggcact gttcatgttg cctgccaggc caagaaataa cgcgacagtt gttaatgggt
    35281 tagctaacgg cagtaacacc accagcccca ggccaattgc tttaaacaaa tctaacattg
    35341 gtggttgtta tcctgtgtat ctgggttatc agcgaaaagt ataaggggta aacaaggata
    35401 aagtgtcact ctttagctaa ccttgcatcg cattggccaa aacttgaacc gatttagcaa
    35461 aacgtggcat cggtcaattc attcatttga cttatacttg cctgggcaat attatcctct
    35521 gcaactaatt acttgccagg gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa
    35581 ttattccatt gggtcgctta atccatatgg ttaatcagaa gaaagatcgt ctgcttaatg
    35641 agtatctgtc tccgctggat attactgcgg cacagtttaa ggtgctctgc tctatccgct
    35701 gcgcggcgtg tattactccg gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac
    35761 tgacccgtat gctggatcgc ctggtctgta aaggctgggt ggaaaggttg ccgaacccga
    35821 atgataagcg cggcgtactg gtaaaactta ccaccagcgg cgcggcaata tgtgaacaat
    35881 gccatcaatt agttggccag gacctgcacc aagaattaac aaaaaacctg acggcggacg
    35941 aagtggcaac acttgagcat ttgcttaaga aagtcctgcc gtaaacaaaa aaagaggtat
    36001 gacgatgtcc agacgcaata ctgacgctat taccattcat agcattttgg actggatcga
    36061 ggacaacctg gaatcgccac tgtcactgga gaaagtgtca gagcgttcgg gttactccaa
    36121 atggcacctg caacggatgt ttaaaaaaga aaccggtcat tcattaggcc aatacatccg
    36181 cagccgtaag atgacggaaa tcgcgcaaaa gctgaaggaa agtaacgagc cgatactcta
    36241 tctggcagaa cgatatggct tcgagtcgca acaaactctg acccgaacct tcaaaaatta
    36301 ctttgatgtc ccgccgcata aataccggat gaccaatatg cagggcgaat cgcgcttttt
    36361 acatccatta aaccattaca acagctagtt gaaaacgtga caacgtcact gaggcaatta
    36421 tgaaaccgct tttatccgca atagcagctg cgcttattct cttttccgcg cagggcgttg
    36481 cggaacaaac ccagcaaccg ctcgttactt cctgtggcga tgtggtggtt gttcccccat
    36541 cgcaggaaca accaccgttc gatttaaatc acatgggtac aggcagtgac aaatcggatg
    36601 cgctgggcgt gccctattat aaccaacaag ccatgtagta tattcaggcc ccggcatatc
    36661 ggggcttgtt aacctcttac ctttaccgct ttacgccacc gcaagccaaa tacattgata
    36721 tacagcccgg tcataatgag caccgcgccg aaaaattgca gacccgttaa gcgttcatcc
    36781 aacaatagtg ccgcacttgc cagtccgact acaggcacca gtaacgataa cggtgcaacc
    36841 cgccaggttt catagcgtcc aagtagtgct ccccagatcc cataaccaac aattgtcgcc
    36901 acaaaagcca gatacatcag agacaagata gtggtcatat cgatagtgac cagactgtga
    36961 atcatggttg cggaaccatc gagaatcagc gaggcaacaa agaagggaat gattgggatt
    37021 aatgcgctcc agattaccag cgacatcacc gccggacgcg ttgagtgcga catgatcttt
    37081 ttattgaaga tgttgccaca cgcccaacta aatgctgccg ccagggtcaa cataaagccg
    37141 agcatcgcca catgctgacc gttcagacta tcttcgatta acaccagtac gccaaaaatc
    37201 gctaaggcga tccccgccaa ttgtttgcca tgcagtcgct caccgaaagt aaacgcgcca
    37261 agcacgatag taaaaaacgc ctgagcctgt aacaccagcg aagccagtcc agcaggcata
    37321 ccaaagttaa tggcgcagaa gagaaaggca aactgtgcaa aactgatggt taatccatac
    37381 cccagcagca aattcagtgg tactttcggt cgtgcgacaa aaaagatggc cggaaaagcg
    37441 accagcataa agcgcaaacc ggccagcatc agcggtggca tgttatgaag ccccactttg
    37501 atgaccacaa aatttagccc ccatacgacc actaccagta gcgccaacac cccatctttt
    37561 cgcgacattc taccgcctct gaatttcatc ttttgtaagc aatcaactta gctgaattta
    37621 cttttcttta acagttgatt cgttagtcgc cggttacgac ggcattaatg cgcaaataag
    37681 tcgctatact tcggattttt gccatgctat ttctttacat ctctaaaaca aaacataacg
    37741 aaacgcactg ccggacagac aaatgaactt atccctacga cgctctacca gcgcccttct
    37801 tgcctcgtcg ttgttattaa ccatcggacg cggcgctacg ctgccattta tgaccattta
    37861 cttaagtcgt cagtacagcc tgagtgtcga tctcattggt tatgcgatga caattgcgct
    37921 cactattggc gtcgttttta gcctcggttt tggtatcctg gcggataagt tcgacaagaa
    37981 acgctatatg ttactggcaa ttacggcctt cgccagcggt tttattgcta ttcccctggt
    38041 ggacagcgtc acgctggtgg tacttttttt cgcactcatc aactgcgcct attccgtttt
    38101 tgccacagtg ttgaaagcct ggtttgccga caatctttca tccaccagca aaacgaagat
    38161 cttctcgatc aactacacca tgctcaatat tggctggacc gtcggcccgc cactcggcac
    38221 gctgttggtc atgcagagca tcaatctgcc cttttggctg gccgctatct gctctgcgtt
    38281 tcccatgttt ttcattcaaa tttgggtaaa gcgcagcgag aaaatcatcg ccacggaaac
    38341 aggcagtgtc tggtcgccga aagttttatt acaagataag gcactgttgt ggtttacctg
    38401 ctctggcttt ctggcttctt ttgttagtgg cgcatttgct tcatgcattt cacaatatgt
    38461 gatggtgatt gctgatggtg attttgccga aaaggtggtc gcggttgttc ttccggtgaa
    38521 tgccgccatg gtagttacgt tgcaatattc cgtgggccgc cgacttaacc cggctaacat
    38581 ccgcgcgctg atgacagcag gcaccctctg tttcgtcatc ggtctggtcg gttttatttt
    38641 ttccggaaac agcctgctat tgtggggtat gtcagctgcg gtatttactg tcggtgaaat
    38701 aatttatgcg ccgggcgagt atatgttgat tgaccatatt gcgccgccag gaatgaaagc
    38761 cagctatttt tccgcccagt ctttaggctg gcttggtgcc gcaattaacc cattagtgag
    38821 tggcctagtg ctaaccaacc tgccgccttc ctcgctgttt atcatcttag cgttggtgat
    38881 tattgctgcg tgggtactga tgttaaaagg gattcgtgca agaccgtgga ggcagcccgc
    38941 gctttgttga tttaagtcga acacaataaa aatttaattc agccttcgtt taggttacct
    39001 ctgctaatat gtttctcatt gagatgaaaa ttaaggtaag cgaggaaaca caccacacca
    39061 taaacggagg caaataatgc tgggtaatat gaatgttttt atggccgtac tgggaataat
    39121 tttattttct ggttttctgg ccgcgtattt cagccacaaa tgggatgact aatgaacgga
    39181 gataatccct cacctaaccg gccccttgtt acagttgtgt acaaggggcc tgatttttat
    39241 gacggcgaaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg
    39301 ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc
    39361 atcagttttc tcaatgaatg ttaaacggag cttaaactcg gttaatcaca ttttgttcgt
    39421 caataaacat gcagcgattt cttccggttt gcttaccctc atacattgcc cggtctgctc
    39481 ttccaatgac cacatccaga ggctcttcag gaaatgcgcg actgacacct gctgtcacgg
    39541 taatgttgat atgcccttca gaatgtgtga tggcatggtt atcgactaac tggcaaattc
    39601 tgacacctgc acgacatgct tcttcatcat tagccgcttt gacaataatg ataaattctt
    39661 cgcccccgta gcgataaacc gtttcgtaat cacgcgtcca actggctaag taagttgcca
    39721 gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc aatttaaatc
    39781 ggtcaatatc caacaacatt aaataaagat tcagaggctc agtgttgcgt aactgatgat
    39841 caaaggattc atcaagaacc cgacgccccg gcaatcccgt caaaacatcc atattgctac
    39901 ggattgtcag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa agtaaccctt
    39961 cctgaaaagc ggcgaaatgc gcgtcctgcc agtgattttc aacaatagcc agcattaatt
    40021 cccgaccaca gttatgcata tgttgatggg cagaatccat tagccgaacg taaggtaatt
    40081 catcgttatc gagtggcccc agatgatcaa tccaccgacc aaactggcac agtccataag
    40141 aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg tgaaacatac
    40201 tcaccagcca ctggtagtgg gcatcgatag ccttattgag atttaacaag atggcatcaa
    40261 tttccgttgt cttcttgatc attgccactc ctttttcaca gttccttgtg cgcgctattc
    40321 taacgagaga aaagcaaaat tacgtcaata ttttcataga aatccgaagt tatgagtcat
    40381 atctgagata acattgtgat ttaaaacaaa atcagcggat aaaaaagtgt ttaattctgt
    40441 aaattacctc tgcattatcg taaataaaag gatgacaaaa agcataaccc aaaccctaat
    40501 ggcccagtag ttcaggccat cgggctaatt tatttttatt tctgcaaatg agtgacccga
    40561 acgacggccg gcgcgctttt cttatccaga ctgccgctaa tgttgatcat ctggtccggc
    40621 tgaacttctc gtccatcaaa aacggccgca ggaataacaa cattaatttc accgctctta
    40681 tcgcgaaaaa cgtaacggtc ctctcctttg tgagaaatca aattaccgcg tagtgaaacc
    40741 gaagcgccat cgtgcatggt ttttgcgaaa tcaacggtca ttttttttgc atcatcggtt
    40801 ccgcgatagc catcttctat tgcatgaggc ggcggtggcg ctgcatcctg ttttaaaccg
    40861 ccctggtcat ctgccaacgc ataaggcatg acaagaaaac ttgctaatac aatggcctga
    40921 aatttcatac taactcctta attgcgtttg gtttgactta ttaagtctgg ttgctatttt
    40981 tataattgcc aaataagaat actgccaatt gttataaggc atttaaaatc agccaactag
    41041 ctgtcaaata ttcagagaat ttaactcact aaagttaaga agattgaaga gtcttaaaca
    41101 tattttcaga a
//
LOCUS       DAZGBC010000021        40497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGBC010000021 DAZGBC010000000
VERSION     DAZGBC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.021"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            260..346
                     /gene="leuL"
                     /locus_tag="ACHK1J_001175"
     CDS             260..346
                     /gene="leuL"
                     /locus_tag="ACHK1J_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO7910478.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            439..2010
                     /gene="leuA"
                     /locus_tag="ACHK1J_001176"
     CDS             439..2010
                     /gene="leuA"
                     /locus_tag="ACHK1J_001176"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO7910479.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2010..3101
                     /gene="leuB"
                     /locus_tag="ACHK1J_001177"
     CDS             2010..3101
                     /gene="leuB"
                     /locus_tag="ACHK1J_001177"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO7910480.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYY
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASTIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3104..4504
                     /gene="leuC"
                     /locus_tag="ACHK1J_001178"
     CDS             3104..4504
                     /gene="leuC"
                     /locus_tag="ACHK1J_001178"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO7910481.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4515..5120
                     /gene="leuD"
                     /locus_tag="ACHK1J_001179"
     CDS             4515..5120
                     /gene="leuD"
                     /locus_tag="ACHK1J_001179"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO7910482.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5228..5359)
                     /gene="sgrT"
                     /locus_tag="ACHK1J_001180"
     CDS             complement(5228..5359)
                     /gene="sgrT"
                     /locus_tag="ACHK1J_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO7910483.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5448..7106
                     /gene="sgrR"
                     /locus_tag="ACHK1J_001181"
     CDS             5448..7106
                     /gene="sgrR"
                     /locus_tag="ACHK1J_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO7910484.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIARIMQQILASHQVTMEIKEISYDQWHKGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPLDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7335..7904)
                     /gene="espY2"
                     /locus_tag="ACHK1J_001182"
     CDS             complement(7335..7904)
                     /gene="espY2"
                     /locus_tag="ACHK1J_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO7910485.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDQLTHWLIK
                     FNELKEHARDPENMAAKASLSPKDTLCVSFFIGDEAIFTLKLQLKENTRTGCIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8146..8245
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8276..9259
                     /gene="thiB"
                     /locus_tag="ACHK1J_001183"
     CDS             8276..9259
                     /gene="thiB"
                     /locus_tag="ACHK1J_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO7910486.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9235..10845
                     /gene="thiP"
                     /locus_tag="ACHK1J_001184"
     CDS             9235..10845
                     /gene="thiP"
                     /locus_tag="ACHK1J_001184"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO7910487.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVIAVALAAFLALWSNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10829..11527
                     /gene="thiQ"
                     /locus_tag="ACHK1J_001185"
     CDS             10829..11527
                     /gene="thiQ"
                     /locus_tag="ACHK1J_001185"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO7910488.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSIVVADGRIAWQGKTEELL
                     SGKASASALLGITG"
     gene            complement(11614..12378)
                     /gene="yabI"
                     /locus_tag="ACHK1J_001186"
     CDS             complement(11614..12378)
                     /gene="yabI"
                     /locus_tag="ACHK1J_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7910489.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12464..13342)
                     /gene="araC"
                     /locus_tag="ACHK1J_001187"
     CDS             complement(12464..13342)
                     /gene="araC"
                     /locus_tag="ACHK1J_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO7910490.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13681..15381
                     /gene="araB"
                     /locus_tag="ACHK1J_001188"
     CDS             13681..15381
                     /gene="araB"
                     /locus_tag="ACHK1J_001188"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO7910491.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHMP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15392..16894
                     /gene="araA"
                     /locus_tag="ACHK1J_001189"
     CDS             15392..16894
                     /gene="araA"
                     /locus_tag="ACHK1J_001189"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO7910492.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17063..17758
                     /gene="araD"
                     /locus_tag="ACHK1J_001190"
     CDS             17063..17758
                     /gene="araD"
                     /locus_tag="ACHK1J_001190"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7910493.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17833..20184
                     /gene="polB"
                     /locus_tag="ACHK1J_001191"
     CDS             17833..20184
                     /gene="polB"
                     /locus_tag="ACHK1J_001191"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO7910494.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGNASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20348..23254
                     /gene="rapA"
                     /locus_tag="ACHK1J_001192"
     CDS             20348..23254
                     /gene="rapA"
                     /locus_tag="ACHK1J_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO7910495.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISVLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDTARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23266..23925
                     /gene="rluA"
                     /locus_tag="ACHK1J_001193"
     CDS             23266..23925
                     /gene="rluA"
                     /locus_tag="ACHK1J_001193"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO7910496.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24043..24858)
                     /gene="djlA"
                     /locus_tag="ACHK1J_001194"
     CDS             complement(24043..24858)
                     /gene="djlA"
                     /locus_tag="ACHK1J_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO7910497.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGDNWQQTQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25113..27467
                     /gene="lptD"
                     /locus_tag="ACHK1J_001195"
     CDS             25113..27467
                     /gene="lptD"
                     /locus_tag="ACHK1J_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO7910498.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27520..28806
                     /gene="surA"
                     /locus_tag="ACHK1J_001196"
     CDS             27520..28806
                     /gene="surA"
                     /locus_tag="ACHK1J_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO7910499.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28806..29795
                     /gene="pdxA"
                     /locus_tag="ACHK1J_001197"
     CDS             28806..29795
                     /gene="pdxA"
                     /locus_tag="ACHK1J_001197"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO7910500.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAILGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLSGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            29792..30613
                     /gene="rsmA"
                     /locus_tag="ACHK1J_001198"
     CDS             29792..30613
                     /gene="rsmA"
                     /locus_tag="ACHK1J_001198"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO7910501.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30616..30993
                     /gene="apaG"
                     /locus_tag="ACHK1J_001199"
     CDS             30616..30993
                     /gene="apaG"
                     /locus_tag="ACHK1J_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO7910502.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31000..31848
                     /gene="apaH"
                     /locus_tag="ACHK1J_001200"
     CDS             31000..31848
                     /gene="apaH"
                     /locus_tag="ACHK1J_001200"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO7910503.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(31845..32159)
                     /locus_tag="ACHK1J_001201"
     CDS             complement(31845..32159)
                     /locus_tag="ACHK1J_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO7910504.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(32162..32395)
                     /locus_tag="ACHK1J_001202"
     CDS             complement(32162..32395)
                     /locus_tag="ACHK1J_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO7910505.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(32481..32960)
                     /gene="folA"
                     /locus_tag="ACHK1J_001203"
     CDS             complement(32481..32960)
                     /gene="folA"
                     /locus_tag="ACHK1J_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO7910506.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLAKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33152..35014)
                     /gene="kefC"
                     /locus_tag="ACHK1J_001204"
     CDS             complement(33152..35014)
                     /gene="kefC"
                     /locus_tag="ACHK1J_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO7910507.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(35007..35537)
                     /gene="kefF"
                     /locus_tag="ACHK1J_001205"
     CDS             complement(35007..35537)
                     /gene="kefF"
                     /locus_tag="ACHK1J_001205"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO7910508.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLDGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGKSHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35648..36979)
                     /gene="yaaU"
                     /locus_tag="ACHK1J_001206"
     CDS             complement(35648..36979)
                     /gene="yaaU"
                     /locus_tag="ACHK1J_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7910509.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(37037..37324)
                     /gene="fixX"
                     /locus_tag="ACHK1J_001207"
     CDS             complement(37037..37324)
                     /gene="fixX"
                     /locus_tag="ACHK1J_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO7910510.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37321..38607)
                     /gene="fixC"
                     /locus_tag="ACHK1J_001208"
     CDS             complement(37321..38607)
                     /gene="fixC"
                     /locus_tag="ACHK1J_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7910511.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38658..39599)
                     /gene="fixB"
                     /locus_tag="ACHK1J_001209"
     CDS             complement(38658..39599)
                     /gene="fixB"
                     /locus_tag="ACHK1J_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7910512.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGTDGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39614..40384)
                     /gene="fixA"
                     /locus_tag="ACHK1J_001210"
     CDS             complement(39614..40384)
                     /gene="fixA"
                     /locus_tag="ACHK1J_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO7910513.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9653 a        11079 c        10687 g         9078 t
ORIGIN      
        1 gttttgcaac gcgtgcattt tgtccccttt ttcctcgtgg attagatgca aaaatttatt
       61 ctgaaatatg tcaaccgatg aaaagcgtcg gtagttaatc agaaattaat atcgcttgtt
      121 ttaaccacag cagcacaatt agctaatttt acggatgcag aactcatgct ggcgggacgt
      181 ttttattgcg tcagggttga catccgtttt tgtatccagt aactctaaaa gcatatcgca
      241 ttcatctgga gctgatttaa tgactcacat cgttcgcttt atcggtctac tactactaaa
      301 cgcatcttct ttgcgcggta gacgagtgag cggcatccag cattaagcca gcacgcagtc
      361 aaacaaaaaa cccgcgccat tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa
      421 gagacaagga cccaaaccat gagccagcaa gtcattattt tcgataccac attgcgcgac
      481 ggtgaacagg cgttacaggc aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc
      541 cttgagcgta tgggtgttga cgtgatggaa gtcgggttcc ccgtctcttc gccgggtgat
      601 tttgaatcag tgcaaaccat cgctcgccag gttaaaaaca gccgcgtatg cgcgttagct
      661 cgctgcgtgg agaaagatat cgacgtggcg gccgaatccc tgaaagtcgc cgaagccttc
      721 cgtattcata cctttattgc cacttcgcca atgcacatcg ccaccaagct gcgcagcacg
      781 ctggacgagg tgatcgaacg cgctatctat atggtgaaac gcgcccgtaa ttacaccgat
      841 gatgttgaat tttcttgcga agatgccggg cgtacaccca ttgccgatct ggcgcgagtg
      901 gtcgaagcgg cgattaatgc cggtgccacc accatcaaca ttccggacac cgtgggctac
      961 accatgccgt ttgagttcgc cggaatcatc agcggcctgt atgaacgcgt gcctaacatc
     1021 gacaaagcca ttatttccgt acatacccac gacgatttgg gcctggcggt cggcaactca
     1081 ctggcggcgg tacatgccgg tgcacgccag gtggaaggtg caatgaacgg gatcggcgag
     1141 cgtgccggaa actgttcact ggaagaagtc atcatggcga tcaaagttcg taaggatatt
     1201 ctcaacgtcc acaccgccat caatcaccag gagatatggc gcaccagcca gttagttagc
     1261 cagatttgta atatgccgat cccggcaaac aaagccattg ttggcagcgg cgcattcgca
     1321 cactcctccg gtatccacca ggatggtgtg ctgaaaaacc gcgaaaacta cgaaatcatg
     1381 acaccagaat ctattggtct gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt
     1441 gcggcggtga aacatcgcat ggatgagatg gggtataaag aaagtgaata taatttagac
     1501 aatttgtacg acgccttcct gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat
     1561 ctggaggcgc tggccttcat cggtaagcag caagaagagc cggagcattt ccgtctggat
     1621 tacttcagcg tgcagtctgg ctctaacgat atcgccaccg ccgccgtcaa actggcctgc
     1681 ggcgaagaag tcaaagcaga agccgccaac ggtaacggtc cggtcgatgc cgtctatcag
     1741 gcgattaacc gcatcactga ctataacgtc gaactggtga aatacagcct gaccgctaaa
     1801 ggtcacggta aagatgcgct gggtcaggtg gatattgtcg ccaactacaa cggtcgccgc
     1861 ttccacggcg tcggcctggc caccgatatt gtcgagtcct ccgccaaagc catggtgcat
     1921 gtactgaaca atatctggcg cgccgcagaa gtcgaaaaag agttgcaacg caaagctcaa
     1981 cacaacgaaa acaacaagga aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc
     2041 cgggggacgg tattggtccg gaagtgatga cccaggcgct gaaagtgctg gatgccgtgc
     2101 gcaaccgctt tgcgatgcgc atcaccacca gccattatta tgtaggcggc gcagccattg
     2161 ataaccacgg gcagccgctg ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg
     2221 tgctgtttgg ctcggtaggc ggtccgaagt gggaacattt accaccagac cagcaaccag
     2281 aacgcggcgc gctgctgcct ctgcgtaagc acttcaaatt attcagcaac ctgcgcccgg
     2341 caaaactgta tcaggggctg gaagcattct gtccgctgcg tgcagacatt gccgcaaacg
     2401 gcttcgacat cctgtgtgtg cgtgaactga ccggcggcat ctatttcggt cagccaaaag
     2461 gccgcgaagg tagcggacaa tatgaaaaag cgtttgatac cgaggtgtat caccgttttg
     2521 agattgaacg tatcgcccgc atcgcatttg aatctgctcg caagcgtcgc cacaaagtca
     2581 cctcaatcga caaagctaac gtgctgcaat cctctatttt atggcgtgaa atcgtcaacg
     2641 agatcgccac ggaatacccg gatgtcgaac tggcgcatat gtacatcgac aacgccacca
     2701 tgcagctgat taaagatcca tcacagtttg acgttctgct gtgctccaac ctgtttggcg
     2761 acattctgtc tgacgagtgc gcaatgatca ctggctcgat ggggatgttg ccttccgcca
     2821 gcctgaacga gcaaggtttt ggactgtatg aaccggcggg cggctcggca ccagatatcg
     2881 caggcaaaaa catcgccaac ccgattgcac aaatcctgtc gctggcactg ctgctgcgtt
     2941 acagcctgga tgccgatgat gcggcttcca ccattgaacg cgccattaac cgcgcattag
     3001 aagaaggcat tcgcaccggg gatttagccc gtggcgctgc cgccgttagt accgatgaaa
     3061 tgggcgatat cattgcccgc tatgtggcag aaggggtgta atcatggcta agacgttata
     3121 cgaaaaattg tttgacgctc acgtagtgta cgaagccgaa aacgaaaccc cgctgttata
     3181 tatcgaccgc cacctggtgc atgaagtgac ctcaccgcag gcgttcgatg gtctgcgcgc
     3241 ccacggtcgc ccggtacgtc agccggggaa aaccttcgcc accatggatc acaacgtttc
     3301 aactcagacc aaagacatta atgcctgcgg tgaaatggcg cgcatccaga tgcaggagct
     3361 gatcaaaaac tgcaaagaat ttggcgtcga gctgtatgac ctgaatcacc cgtatcaggg
     3421 gatcgtccac gtgatggggc cggaacaggg cgtgaccttg ccggggatga ccattgtctg
     3481 cggcgactcg cacaccgcca cccacggcgc gtttggcgca ctggcctttg gtatcggcac
     3541 ttccgaagtt gaacacgtac tggcaacgca aaccctgaaa cagggccgtg cgaagaccat
     3601 gaaaattgaa gtccagggca aagccgcgcc gggcattacc gcaaaagata tcgtgttggc
     3661 aattatcggt aaaactggca gcgcaggcgg caccgggcat gtggtggagt tttgcggcga
     3721 agcaatccgt gatttaagca tggaaggtcg gatgaccctg tgcaatatgg caatcgaaat
     3781 gggcgcgaaa gccggtctgg ttgcaccgga cgaaaccacc tttaactatg tcaaaggtcg
     3841 tctgcatgcg ccgaaaggca aagatttcga cgacgccgtt gcctactgga aaaccctgca
     3901 aaccgacgaa ggcgcaactt tcgataccgt tgtcactctg caagcagaag agatttcgcc
     3961 gcaggtcacc tggggcacca atccaggcca ggtgatttcc gtgaacgaca atattcccga
     4021 tccagcttcg tttgccgatc cggttgaacg cgcgtcggca gaaaaagcgc tggcctatat
     4081 ggggctgaaa ccgggtattc cgctgaccga agtggctatc gacaaagtgt ttatcggttc
     4141 ctgtaccaac tcgcgcattg aagatttacg cgcggcagcg gagatcgcca aaggccggaa
     4201 agtcgcgcca ggcgtgcagg cactggtggt tcccggctct ggcccggtaa aagcccaggc
     4261 ggaagcggaa ggtctggaca aaatctttat tgaagccggt tttgaatggc gcttgcctgg
     4321 ctgctcaatg tgtctggcga tgaacaacga ccgtctgaat ccgggcgaac gttgtgcctc
     4381 caccagcaac cgtaactttg aaggccgtca ggggcgcggc gggcgcacgc atctggtcag
     4441 cccggcaatg gctgccgccg cggctgtgac cggccatttc gccgacattc gcaacattaa
     4501 ataaggagca caccatggca gagaaattta tcaaacacac aggcctggtg gttccgctgg
     4561 atgccgccaa tgtcgatacc gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc
     4621 gtacgggttt tggcgcgcat ctgtttaacg actggcgttt tctggatgaa aaaggccaac
     4681 agccaaaccc ggacttcgtg ctgaacttcc cgcagtatca gggggcttcc attttgctgg
     4741 cgcgggaaaa cttcggctgt ggctcctcgc gtgagcacgc gccctgggca ttgaccgact
     4801 acggttttaa ggtggtgatt gcgccgagtt ttgctgacat cttctatggc aatagcttta
     4861 acaaccagct gctgccggtg aaattaagcg acgcagaagt ggatgagctg tttgcgctgg
     4921 tgaaagccaa tccggggatt cattttgatg tagacctgga agcgcaagag gtgaaagcgg
     4981 gtgagaaaac ctatcgcttt accattgatg ccttccgccg ccactgcatg atgaacggtc
     5041 tggacagcat tgggcttacc ttgcagcacg acgacgccat tgcggcttat gaagcgaagc
     5101 aacccgcgtt tatgcgttag tttgtacacc gtccggtact caatcgccaa aaagaaaaaa
     5161 gccagcagat aatcactgct ggcgtggcga ttaacaccaa tactcagtca cacatgatgt
     5221 ccgcaaatca gtagtcagaa tgactcccct cccactgcat cagttcttca agcatggtta
     5281 atcgttgtgg gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt
     5341 gctgataaaa ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa
     5401 tatagggaaa ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc
     5461 gtctgcaaca acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa
     5521 cgctcaacga actggcagcg ttattgagct gctcgcgtcg tcatatgcgc accctgctca
     5581 acaccatgca ggatcgcggc tggctgacgt gggaagcgga agtcgggcgt ggtaaacgct
     5641 cgcgtctgac attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc
     5701 tggagcagga ccgtatcgac caactagtgc agttagttgg cgacaaagcg actgtgaggc
     5761 aaatgctggt ttctcatctg ggccgtagct tccgccaggg gcggcacatc ctgcgcgtgc
     5821 tctactatcg tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc
     5881 atatcgcccg gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg
     5941 aagcagacat cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc
     6001 gtccaggagt ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt
     6061 taaaacgaat caatacgctg ccgctctatt cgcatattgc tgacattgtg tcgccgacgc
     6121 cctggacgct ggatatccac ctcacgcagc cggatcgctg gttgccgtta ctgctgggac
     6181 aagttccggc gatgatcctg ccgcgcgaat gggaaaccct cagtaacttt gccagccatc
     6241 ccatcggcac cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg
     6301 cattcgatga cttcttcggt taccgggcat taatcgatga agttaatgtc tgggttctgc
     6361 cggaaattgc cgacgagcca gccggagggc tgatgctgaa aggcccacag ggcgaggaaa
     6421 aagagattga aagccgactg gaggaaggtt gttactattt actgttcgac agccgaaccc
     6481 atcgcggggc gaatcaacaa gtcagagact ggataagcta tgtgctttcg ccgaccaatc
     6541 tggtctattt cgccgaggaa cagtaccagc aactgtggtt tccggcctat gggctgctcc
     6601 cccgctggca ccacgcccgc ccaacacatt gcgaaaaacc tgccgggctg gaacatctca
     6661 ccctgacgtt ttatcaggat catattgaac atcgggtgat tgccaggatc atgcagcaga
     6721 ttctggcgag tcatcaggtc acgatggaaa tcaaagagat cagctacgat caatggcaca
     6781 aaggtgagat cgaaagcgac atatggctta acagcgccaa ctttacgctg ccgctggatt
     6841 tttcgctgtt cgcgcacttg tgcgaagtac ccctgctgca acactgtatt ccgctcgact
     6901 ggcaagccga cgcagcccgc tggcgcaatg gcgagataaa tctggcgaac tggtgccagc
     6961 aactggtcgc cagtaaagca atggtgccac ttatccacca ctggctgatc attcaggggc
     7021 aacgcagtat gcgcggcctg cgcatgaata ccctcggctg gttcgatttt aaatcagcgt
     7081 ggtttgcgcc gccggatcca gagtagcttg cgccttttgg cggtcatgag cgcatttttt
     7141 atattcagac ggaatgataa aatacattac ttttatattt tctctgtttt atgcatcgtc
     7201 tgattagcgt cgcaaataag attaaagaag gggcattcca gcccctcttt catattctga
     7261 gttaatcaat aacggcgcag tctagccttg ttctggccgg aaagatcatc tttttcaggt
     7321 taacctcgcg caggtcagtc ataccaacgg ctattgttcg tttcagcatt gctcaggtca
     7381 acctcgagac agtcaatacc acaaatgaca accccgttaa agtaagcatt actcagatca
     7441 atgcacccgg tcctggtgtt ctctttcagc tgcaacttca gagtaaatat cgcctcatcg
     7501 ccaataaaaa aactgacgca taacgtatct tttggcgaga gtgaggcctt cgcggccata
     7561 ttttccggat ctcttgcgtg ttctttgagt tcattaaatt ttatcagcca gtgagttaac
     7621 tgatcacgcg ccttcccgtc gacagccccg gtaaagtatt ttgagtagtc atcactattc
     7681 tggaaataaa atgcatcctt cagaatttgc cttgccgcag tctgggaatg tttatctgac
     7741 cggaagagat tgcgtaacgt ctcccatagc tcttcaaaga tattcatatt ctcggtcttt
     7801 ttgaccaagt tagccatcct gataatctga gaattagtaa ggcctggatc tttagtggta
     7861 ttactagcag ggatactaat ctgttctggg tttcttactt tcatgctgat agctccactc
     7921 tgagtaaaaa actcagaata agcgtcctga ataaggatac tgtcaaaaat cgctcattca
     7981 cgcaataaac ggttacgtta cctattttga ctgtagcgaa gggattcttt cggatcaacg
     8041 ctctccagct atgcaaatac gctcttgata gctgaacgaa tattggcttt tttaatacgc
     8101 cttctctatg gattgctggt tgataacaaa atcactacac taacgccgtt ctcaacgggg
     8161 tgccacgcgg acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
     8221 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
     8281 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
     8341 gactgtttat acctacgatt ccttcgccgc cgactggggg ccaggtccgg tggttaaaaa
     8401 agcctttgaa gccgactgta attgcgaact gaaactggtg gcgctggaag atggcgtttc
     8461 gcttctcaac cgtctacgga tggaaggcaa aaacagtaaa gccgatgtgg tgctggggct
     8521 ggataacaac ctgttagacg ccgccagtaa aaccggactg tttgccaaaa gcggtgtggc
     8581 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtac cgtttgatta
     8641 tggctacttc gccttcgttt atgacaagaa caaactgaaa aacccgccac aaagcctgaa
     8701 agaactggtt gagagcgatc aaaactggcg ggtgatttat caggatccgc gcaccagtac
     8761 accggggctg ggtctgttgc tatggatgca aaaagtctat ggcgatgacg ccccacaagc
     8821 ctggcagaaa ctggcgaaga aaaccgtcac cgtcaccaaa ggctggagcg aagcctacgg
     8881 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
     8941 cattctcgaa gagaagaaag ataactacgc cgccgcgaac ttcagcgaag gtcactatct
     9001 gcaagtggaa gtcgccgctc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
     9061 cctccagttt atggtttctc cggctttcca gaatgcgatc ccaaccggca actggatgta
     9121 tccggtggca aacgtcacgc tgcctgccgg ttttgaaaaa ttgaccaaac ccgcaaccac
     9181 gctggagttc acgccagccg aagtggcggc acaacgtcag gcatggatta gcgaatggca
     9241 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
     9301 gtgatagcgg ttgcgctggc ggcgtttctc gccctgtggt cgaacgcgcc gcagggtgac
     9361 tggtcggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
     9421 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgcgctctat
     9481 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
     9541 ccggtgctgg ttgctgtttt cggcattctt agcgtctatg gtcgccaggg ctggctggca
     9601 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggtatt
     9661 ttgctggcgc acgtattttt taatctaccg atggcgagcc gcttattact ccaggcactg
     9721 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
     9781 tttttccgct tcgtcgaatg gccgtggtta cggagacaaa tcccgccggt tgctgcgctt
     9841 atctttatgc tctgtttcgc cagcttcgcc accgtactgt cgctgggcgg cggtccgcag
     9901 gcgaccacta tcgagctggc aatctatcag gcgctgagtt acgactacga tcctgcccgc
     9961 gcggcgatgc tggcgctgat ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    10021 cgactgagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgacccggac
    10081 gatcgtctgc atagccgcat ttgcgacacg gtgttaattg tgctggcgct gctgctgttg
    10141 cttccaccgt tactggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    10201 gcacaaccgg tactgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggc
    10261 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    10321 cagaaaatgc tggcgggtca ggcgctggag atgagtggca tgttgatcct cgccatgccg
    10381 gggattgtgc tggcaacggg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    10441 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccccta cgccctgaaa
    10501 gtgctggaaa acccgatgcg cgatatcacc gcccgctaca gcatgttatg tcagtcgctg
    10561 gggattgaag gctggtcacg cttaaaagtg gtggagctgc gcgccctgaa acgtccactg
    10621 gcgcaggcgc tggcctttgc ctgcgtgctg tcgattggtg attttggcgt ggtagcgttg
    10681 ttcggtaacg atgatttccg caccctgccg ttttatctct accagcaaat tggctcctat
    10741 cgcagccagg acggcgcggt caccgcgtta attctgctgc tactctgttt tctgctgttt
    10801 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    10861 ccaccatttg ccgatgcgtt ttagcttaac agtagaacgc ggcgagcagg tggcgatcct
    10921 cgggccaagc ggcgcaggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    10981 agccagcggt tcgctgacta tcgatggcgt tgatcacacc acgacgccgc cgtcacgccg
    11041 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc cacctgacgg tcgcgcagaa
    11101 catcgggctg gggctaaatc cgggattgaa actgaacgcg gcacagcagg agaaaatgca
    11161 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttacctg gcgagctttc
    11221 cggcggtcag cgacagcgag tggcgttagc gcgttgtctg gtacgcgaac agccgatttt
    11281 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgttgacgct
    11341 ggtgagcacg agctgccagc agcaaaaaat gacgttattg atggtgtcgc acagtgtgga
    11401 agatgcggca aggattgcaa cacgctcgat agtggtcgcc gacgggcgca tcgcctggca
    11461 gggtaagacc gaagaattgt tgagcggtaa ggcgagtgct tcggcgctgt tgggaattac
    11521 aggttaatat gcactgtcgg atgcggcgtg aacgccttat ccgacctacg ggtgcgtttt
    11581 gtccgcatca agcgccccac acaagactat gccctaaact ccaaccactt tacgcaaaat
    11641 atcgatatac accggcatta acggatggcg aattaacacc accagcgcca ccacgccgat
    11701 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
    11761 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
    11821 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcattccgg ctggaatatc
    11881 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
    11941 gataatattc ggcgtaataa atttagccac cggcagatcc agcattcccg ccaccattgg
    12001 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
    12061 atgcaacgca tgttcagttt tatcgagtaa tgctttgttc ttcttcagaa atgaccagcg
    12121 atgcaatggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcagaca
    12181 accgacgatc cctgccagcc aggcgtgcca gaaacttaac tcgccgctgc caatcagcgc
    12241 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
    12301 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
    12361 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
    12421 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
    12481 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
    12541 attttttaaa tactcgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
    12601 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgatg cgttggtcct
    12661 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
    12721 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
    12781 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
    12841 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
    12901 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
    12961 gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc aaatattgac ggccagttaa
    13021 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
    13081 cctccggatg acggccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
    13141 gtcggcagac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
    13201 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
    13261 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
    13321 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
    13381 gtccatattg catcagatat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
    13441 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
    13501 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat aatttgcacg
    13561 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
    13621 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
    13681 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggac
    13741 tgcgccagcg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcaaaaaggg
    13801 caattttgtg atgccccgaa taaccagttc cgtcatcatc cgcgtgacta cattgagtca
    13861 atggaagcgg cactgaaaac cgtgcttgca gagcttagcg tcgaacagcg cgcagctgtg
    13921 gtcgggattg gcgttgacag taccggctcg acgcccgcac cgattgatgc cgacggtaac
    13981 gtgctggcgc tgcgcccaga gtttgccgaa aacccgaacg cgatgttcgt attgtggaaa
    14041 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
    14101 gttgactact cccgctacat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
    14161 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
    14221 tgcgactggg tgccagctct gctttccggt accacgcgcc cgcaggatat tcgtcgcgga
    14281 cgttgcagcg ccgggcataa gtcgctatgg cacgaaagct ggggcggcct gccgccagcc
    14341 agtttctttg acgaactgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
    14401 gatacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
    14461 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
    14521 ggtgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
    14581 ctgattgccg acaaacagag cgttggcgag cgggcagtga aaggtatttg cggtcaggtt
    14641 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
    14701 atctacgcct ggttcggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
    14761 ccggaactaa aagagcaaat taacgccagc cagaaacaac tgcttccggc gctgaccgaa
    14821 gcatgggcca aaaatccgtc tctggatcac atgccggtgg tgctcgactg gtttaacggc
    14881 cgccgcacac cgaacgctaa ccaacgcctg aaaggggtga ttaccgacct taacctcgct
    14941 accgatgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
    15001 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    15061 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    15121 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    15181 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    15241 accctgcaac cgtgcagcga gcaggcacaa cgctttgaac agctttatcg ccgctatcag
    15301 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    15361 caggccgttc cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    15421 gtttgtaatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    15481 tgccgagcac gttgttaatg cgctaaatac ggaagcgaaa ctgccctgca aactggtgtt
    15541 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    15601 cgatcgttgc gctggtctgg tggtatggct gcacaccttc tccccggcca aaatgtggat
    15661 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt tcaacgcggc
    15721 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    15781 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    15841 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgtcagg cggtctctaa
    15901 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtggcggt
    15961 caccgatggc gataaagttg ccgcacaaat caagttcggt ttctccgtca atacctgggc
    16021 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    16081 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    16141 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aaacgtttcc tggaacaagg
    16201 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    16261 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    16321 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    16381 ctttatggag gactacacct atcacttcga gaaaggcaat gacctggtgc tcggctccca
    16441 catgctggaa gtctgcccgt cgatcgccgc agaagagaaa ccgatcctcg acgttcagca
    16501 tctcggtatt ggtggtaagg acgatcctgc ccgcctgatc ttcaataccc aaaccggccc
    16561 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctactgg ttaactgcat
    16621 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    16681 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgcaggtg gcgcgcacca
    16741 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    16801 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcgctgcg
    16861 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc gcatcaggtg tgtaacgcct
    16921 gatgcgacgc tgacgcgtct tatcaggcct acacgctgtg attttgtagg ccggataagc
    16981 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    17041 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    17101 tttggcgctg ccaaaacata acctggtcac gctcacctgg ggcaacgtca gcgccgttga
    17161 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagca tcatgaccgc
    17221 cgacgatatg gtcgtggtca gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    17281 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccgtcca ttggcggcat
    17341 tgtgcacaca cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc
    17401 aactggtacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    17461 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    17521 cgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt ctcacggccc
    17581 atttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    17641 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    17701 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    17761 actgtataaa accacagcca atcaaatgaa accaggctat aatcaagcct ggttttttga
    17821 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggataccccg
    17881 caaggaacag aggtctcctt ctggctggcg acggacaacg ggccgttgca ggttttcctt
    17941 gcgccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    18001 ttgcggggtg aacaaggctt tcgcctgacg ccgctggcgt taaaggattt tcaccgccag
    18061 ccggtgtatg gcctttactg tcgcgcccat cgccagttga tgaattacga aaagcgcctg
    18121 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    18181 gagcggttta tcacctcgcc ggtgtgggtc gagggtgata tgcacaatgg cgctatcgtt
    18241 aatgcccgtc tgaaaccgca tcccgactat cgcccgccgc tcaagtgggt gtctattgat
    18301 attgaaacca cccgccacgg tgagctgtat tgcatcggcc tggagggctg cgggcagcgc
    18361 atcgtttata tgctggggcc ggagaatggc aacgcttccg cgctcgattt cgaactggaa
    18421 tacgtcgcca gtcgcccgca gctactggaa aaacttaacg cctggtttgc caactacgat
    18481 cctgatgtga tcatcggttg gaacgtggtg caattcgatc tgcgaatgct gcaaaaacat
    18541 gccgagcgtt atcgtattcc gctgcggctg gggcgcgata acagcgagct ggagtggcgc
    18601 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aagggcggct aattatcgac
    18661 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    18721 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    18781 attgaccgcc gtttcgccga agataaacct gcgctggcga cttataacct gaaagattgc
    18841 gagctggtga cgcagatttt ccataaaact gaaatcatgc cgtttttgct cgaacgagca
    18901 acggtgaacg gcctgccggt agaccgccac gggggatcgg tggcggcgtt tggtcatctc
    18961 tattttcccc ggatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    19021 cacgccagcc ctggcggcta cgtgatggat tcgcgacctg ggctttatga ttcggtgctg
    19081 gtgctggact acaaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    19141 gggctggtgg aaggcatggc gcagcctgat ccagagcaca gcaccgaagg ctttctcgat
    19201 gcctggttct cgcgagaaaa acattgcctg ccggagattg tgactaacat ctggcacggg
    19261 cgcgatgaag ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa gatcattatg
    19321 aatgcctttt atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcggctggca
    19381 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcgtt gattgaagcg
    19441 cagggctacg acgtgatcta cggcgatact gactcaacgt ttgtctggct gaaaggcgca
    19501 cattcggaag aagaagcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    19561 tgggcggaaa cgctgcaaaa acaacagctg accagcgcac tggaactgga gtatgaaacc
    19621 catttctgcc gttttctgat gccaaccatt cgcggagccg ataccggcag taaaaagcgt
    19681 tatgccgggc tgattcagga gggcgacaaa cagcggatgg tgtttaaagg gctggaaacc
    19741 gtgcgtacgg actggacgcc actggcccag cagtttcagc aggagcttta cctgcgtatc
    19801 ttccgcaacg agccatatca ggagtacgtc cgcgaaacca tcgacaaact gatggcgggt
    19861 gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag
    19921 cgtaatgttc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgc
    19981 ggtcgcccct tgcaatacca gaaccgcggc accattaagt acgtatggac caccaacggc
    20041 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    20101 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    20161 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    20221 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    20281 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    20341 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    20401 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    20461 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    20521 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    20581 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    20641 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgcc tgtttgccgg
    20701 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    20761 acagttccgt atgccgtaca gcggcttgcg cggtcagcgt accagcctga tcccgcacca
    20821 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    20881 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    20941 cgctgctgaa cgtgtgctga ttattgtccc ggaaacctta cagcatcagt ggctggtaga
    21001 aatgctgcgc cgtttcaacc tgcgttttgc gctgtttgat gatgagcgtt atgccgaagc
    21061 tcagcacgat gcttacaacc cgttcgatac cgaacaactg gtgatttgct cgctggattt
    21121 tgcccgtcgt agcaaacagc ggctggaaca tctctgtgaa gccgaatggg acctgctggt
    21181 ggtcgatgaa gcccatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    21241 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    21301 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    21361 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    21421 gctgctggca ggtaataaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    21481 cgagcaggat atcgagccgc tgttgcaagc agcaaacagc gacagcgaag atgcccagag
    21541 cgcccgtcag gaactggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    21601 ccgtaacacc cgtaacggtg tgaaaggctt cccgaaacgc gagttgcaca ccattaagct
    21661 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    21721 aagtgcggaa gatcgtgctc gagatatgct ctacccggag cgtatttatc aggaatttga
    21781 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    21841 gaccagccat cgctctcaga aagtgctggt gatctgcgct aaagctgcca ctgcgctgca
    21901 actggagcaa gtactgcgcg aacgtgaagg tatccgcgct gctgtgttcc acgaaggcat
    21961 gtcgattatt gagcgtgacc gcgctgccgc ctggtttgct gaagaagaca ccggcgcaca
    22021 ggtactgctg tgctcggaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    22081 ggtgatgttt gacctgccat tcaacccgga tctgctggag cagcgtattg gtcgtctgga
    22141 tcgtatcggt caggcgcacg atattcagat ccatgtgcct tatctggaaa aaactgctca
    22201 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca ttcgagcaca cctgcccgac
    22261 cggacgcact atttacgata gcgtctacaa cgatctgatt aactatctgg cttcaccgga
    22321 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    22381 agcacaactg gaacaggggc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    22441 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    22501 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    22561 cgtgctgacc ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    22621 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    22681 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    22741 cagcacgatt tcagtgttga aaaacaaagc gttaccggta ggtacgctgt tggtggaact
    22801 gatttacgtg gtcgaagccc aggcgccgaa gcagttgcag ctcaaccgct tcctgccacc
    22861 gacgccggta cgtatgctgc tggataaaaa tggcaacaac ctggcggccc aggtggagtt
    22921 tgaaaccttt aaccgccagc ttaacgcggt caaccgtcac accggcagca aactggttaa
    22981 tgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    23041 ggcccgtgca ttgattgata ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    23101 gtctcgtctg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    23161 cattgaaagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    23221 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    23281 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    23341 gtcaacaagc cgagcggttt gttgtctgtg ccgggtcgtc tggaagagca caaagacagc
    23401 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    23461 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    23521 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    23581 cctgcggaag gtctggtaga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    23641 aaagtctgtt acgaaacagg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    23701 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    23761 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccggaa
    23821 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    23881 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatagtc acactggcga
    23941 agttcggttg gccggagaag gcgttcacgc cgcatccggc aattttgcgc cgattgcctg
    24001 atgcgacgcg ggccgcgtct tatcaggcct acagtttgaa acttatttaa accctttctg
    24061 ctgcttaatc aattcatagg cctgctgaat ttcctgcgct ttttgcttcg ccatctccat
    24121 catctcaggc ggcaaacctt tcgccaccaa cttatcggga tggtgttcac tcatcagctt
    24181 acggtaggca cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca
    24241 ggcatcttcc agcgtcgggc cacgctgggt ttgttgccag ttatcaccgc cagattgctg
    24301 ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg
    24361 ggcgcgggag atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg
    24421 cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc
    24481 aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattgtc
    24541 tgattttccc acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat
    24601 tcggtccatc aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt
    24661 ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg
    24721 gttggcaaac cacgccattt tacggctacg ggctttatca aacatatggc caattaacag
    24781 gcctaacact acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat
    24841 ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca
    24901 caatataaag gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa
    24961 tttgcattat catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca
    25021 ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa
    25081 cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg
    25141 ccaccatgat tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac
    25201 agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact
    25261 tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta
    25321 ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc
    25381 atcaaaaaga ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg
    25441 tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca
    25501 ccaaagatac caacgtctgg gagggtgatt accagatggt gggtcgccag ggccgcggta
    25561 aagcggacct gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct
    25621 ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg
    25681 accgcgaaga acaagtggcc gaaatctgga acgcccgttt taaagttggc ccggtaccaa
    25741 tcttttatag cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga
    25801 tcccgaacgc caagtacacc accaccaact actttgagtt ctacctgcca tattactgga
    25861 acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca
    25921 tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg
    25981 actatctgcc ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc
    26041 gttggttatt ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg
    26101 actacaccaa ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca
    26161 gtactgacgg ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg
    26221 ccaccgtttc aaccaagcag ttccaggtct tcagcgaaca gaacaccagt agctactcgg
    26281 cagagccgca gttagacgtt aactactacc agaatgatgt tggtccgttt gatacgcgta
    26341 tttacggcca ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg
    26401 ttcacctgga accgaccatc aatttgccgc tctctaataa ctggggcagt atcaataccg
    26461 aagcgaagtt gctggcaacc cattatcagc aaactaatct tgactggtat aactccagaa
    26521 acacgaccaa gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca
    26581 aaatggtctt tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac
    26641 cgcgcgcgca gtatttgtac gtgccatatc gcgatcagag cgacatctat aactacgact
    26701 catctctgct gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg
    26761 accgtattgc ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg
    26821 ctgccgttga acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca
    26881 ctggcgatga caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag
    26941 gcgatactta ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata
    27001 cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc
    27061 gtctggtaca gctgaattac cgttacgcca gcccggaata tattcaggct acgctgccta
    27121 agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca
    27181 gctggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga
    27241 acaagcaagc cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg
    27301 tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca
    27361 acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caattacggt ctgggtacgc
    27421 aagagatgct gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac
    27481 cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg
    27541 cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa
    27601 gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag
    27661 tcggtaaaac tgaacgctgc tcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc
    27721 caccaaatca tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg
    27781 ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac
    27841 aacatgacgc tggatcagat gcgcagccgt ctggcctacg atggtctgaa ctacaacacc
    27901 tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt
    27961 cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa
    28021 aacgacgcca gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg
    28081 acctctgatc aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt
    28141 aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac
    28201 ggcggccaga tgggctgggg ccgtattcag gagttgccgg ggatcttcgc ccaggcatta
    28261 agcaccgcga agaaaggcga cattgttggt ccgattcgtt ccggcgttgg cttccatatt
    28321 ctgaaagtta acgaccttcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct
    28381 cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg
    28441 gaacagattg ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag
    28501 ttctcgcagg atccaggttc tgctaaccag ggcggcgatc tcggctgggc tacaccagat
    28561 attttcgatc cagccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca
    28621 ccggttcact cttcattcgg ctggcattta atcgaactgc ttgatacccg taatgtcgat
    28681 aaaaccgacg ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg
    28741 gaagaagcag caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc
    28801 aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc
    28861 cggacttagt tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg
    28921 atgccactct ccttaccgac cgggcagcga tactcggttt gccgctcacc ctccgccctt
    28981 attcccccaa ctcccctgca caaccgcaaa ctgcgggcac attaacgcta cttcctgtcg
    29041 cgctacgaga atctgtcact gcggggcagt tagcggttga aaatgggcat tacgtggtgg
    29101 agacgctggc gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag
    29161 gccccgtgca taaaggcgtc attaacgacg caggcattcc gtttaccggt cataccgagt
    29221 ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc
    29281 gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagacgct atcacccctg
    29341 cacttttgca cgaagtgatt gctattttgc atcacgattt gcggaccaaa tttggtattg
    29401 ccgaaccgcg cattctggtc tgtgggctga atccgcacgc gggcgaaggc ggtcatatgg
    29461 gtacggaaga gatagacacc attattccgg tgctcgacga actgcgggcg caggggatga
    29521 aacttagcgg gccgctgcct gctgataccc tgtttcagcc taaatatctc gataacgccg
    29581 acgcggtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg
    29641 ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca
    29701 ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta
    29761 atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt
    29821 agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt
    29881 ttctgccatt aacccgcaga agggccaggc gatggtcgaa atcggccccg gtctggctgc
    29941 attgaccgaa ccggtcggcg aacgtctgga ccagctgacg gttatcgaac ttgaccgcga
    30001 tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca
    30061 ggatgcgatg acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt
    30121 tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac
    30181 tgatgccatt gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc
    30241 aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaatgt
    30301 gatcccggta ctggaagtac cgccgtcagc ctttacacca ccacccaaag tggattccgc
    30361 cgtcgtgcgc ctggttcctc atgcaacgat gcctcacccg gttaaagatg ttcgcgtgtt
    30421 gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg
    30481 caacctgttt agcgtcgagg tgttaacggg aatggggatc gacccggcga tgcgagcgga
    30541 aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt
    30601 gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac
    30661 attgaggctc aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata
    30721 cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc
    30781 aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg
    30841 cctggcgaag agtaccagta caccagcggt gcaatcattg aaacgccgct gggcaccatg
    30901 cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta
    30961 ttccgactcg ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc
    31021 gacgttcatg gttgttacga tgaactgatc gcattgctgc ataaagtaga atttacccct
    31081 gggaaagata ccctctggct gacaggcgat ctggtcgcgc gcggcccggg ttcgctggat
    31141 gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat
    31201 ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca
    31261 ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctctg
    31321 ctgcaaatcg acgaagagaa aaagctggtg atggcccacg cagggatcac gccgcagtgg
    31381 gatctacaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc gagtgactcc
    31441 tatcccttct ttctcgacgc tatgtacggc gatatgccaa ataactggtc accggaattg
    31501 cgggggctgg gaagattgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttt
    31561 ccaaacggcc aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg
    31621 aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac
    31681 tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt acgcgctgga taccggctgc
    31741 tgctggggtg ggtcattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg
    31801 tcgaaccggc ataaggattt gggtgaggga gaggcggtcg cgtcttaaat cccgtcgagc
    31861 ataaaatcta atgctccttt aatcaacgtt cggtgcgcgc tggcatcgca aaatttcgtt
    31921 cccagcgcgt taactggaac agttgcagtc tcatgcgtca tgagcacata ttctttgccg
    31981 tctaccagta aaaggattgg gttaagccgt tctggtgggc gaatacggct aaatcgttca
    32041 ataggtgtta atggaataac aatacggcga ttaatcacac caataatgtc gctggtaaca
    32101 tcgataacaa agggaaacgc ggcgttcctg ctgcgactgc ggtataccgt aaattgcatc
    32161 gctagaagct ccggtactca tcagagaaac atccggtttc atcagccaat tgatttaacg
    32221 cagcgatagc ttctgcgttc tcttcacgcc aacgtgttgc tgcatgtttt ttaagttcag
    32281 cattgagcgc aaccgtaagg gtggcgctca tatttaagcc tgcatcacga gcgcgattga
    32341 ctaactcacg gtcgacggtg acggtcacac tttgtgtggt acgttttgca gtcatgatat
    32401 cgtgctcctg tacaatttat acgtctagta tacacggata tctgcacgta tcagtgatgc
    32461 cggaattcta atatacaaaa ttaccgccgc tccagaatct caaagcaata gctatgagag
    32521 ttctgcgcat cagcgtcgtg gaattcgctg aataccgatt cccagtcatc cggctcgtaa
    32581 tccgggaaat gggtgtcgcc ttccacttct gcgtcgatat gcgtcagata cagtttttgc
    32641 gcttttgcca ggaactgctc ataaacgcga ccgccgccaa tcaccatgat ttctggtacg
    32701 tcaccacacg ccgcgatggc ttcatccacc gacttcaccc acgttacgcg atcgtccgta
    32761 cccggttgac tgctgaggat aatatttttg cgtcctggca acggacgacc gatggattcc
    32821 caggtatggc ggcccataat cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga
    32881 tcggcaggca ggttccacgg catggcgttt tccatgccga taacgcgatc taccgctaac
    32941 gccgcaatca gactgatcat tgagatttcc cgataaaaaa aattgtcgcc actatacgta
    33001 aagcgtaaac cgtcgtcgac tggtgcgagg atgatgttga ggaaaatttt atattctgct
    33061 ggcgagtcca cgctctcccc ctggactcgc cgcattacaa tgaaacaaaa acaaacagtt
    33121 agctgtaaag tgtgatttac gtcactcttt attaggatga gggtttcgtt tccggttcat
    33181 ccgccatgtt gccggtatgt ttaccttctt ctgttccctg ccagccatgt cgttgaatta
    33241 atgacagatg ttcgcggtcc tcggtgatga tctcacttaa catcgcgctg gtgcgtttat
    33301 agaccgccgc gcgggctttg gtgtcattct caaccattgc catctcttcc accatctgaa
    33361 tattaaagcg gcggaacaca tcggcacgtt ctcgcgcttc atacggcccc agacctaaac
    33421 tttccagcgc cagacgcccg gttttcagcg caccttcaaa ggtttcacgc tccggctttt
    33481 caacgcctgc ctgacgcaaa cgaatgtagt ggtcgacatc gcgggcgcgg gcaataatct
    33541 gcaaatgcgg gaaatgttct ttcaccatct ccgtcagttg caggttggtt tgcggatcgt
    33601 caatagcgtt aatcagcact tccgctttcg ctgctccggc agattccagt aaatccatcc
    33661 gcgtggcatc gccgtaaaac actttcatac caaatttacg cagcgtttcg atatggtccg
    33721 gatcgtgatc gagcaccacc attttcaccc cgctggagag cagtaaacgt ccggtaatct
    33781 gtccaaaacg accgaatccg gcgataatca cgcgcggctg ttcttcatcg atctcatcgg
    33841 cttcacgcgc ttcctcagta gaagattgct caaggcgatt gaggatcacc agcagaatcg
    33901 gcgttgctgc catcgacagc gccaccgcca gggtcagcga tttcgcccac tccggctcca
    33961 gcacattcgc catctgcgcc gcgccaaata ccacaaaggc aaactcactg ccctgcccta
    34021 acaacaccgc aaaccagcga cgctgtttat ttggcacctg caacggtcgg gcaatcagcc
    34081 acagcatggc gattttgatg atgaggaaac cgagcagcaa aatgacaatg cgcaatgggt
    34141 tttcaatcag cgtgccaaag tctatcgaca tgccaacacc gatgaaaaac agccccaaca
    34201 gcaaaccttt aaacggttcg atatcgcttt ccagcgcatg acggtattcc gagcttgcca
    34261 gcagtacgcc cgccagaaac gcgcccatcg ccatcgacaa gccgacctct tccagcagca
    34321 aaccaaagcc aaacacaagg aataacgcca cggcactaaa cacttcccgc aagccagagc
    34381 gggcaacaaa acgcagcgcc ggacgcgtaa catagcgccc cagcaatacc accagtacca
    34441 gcgcacccgc cacttttaac gccgagagag caaatgcgcc catcgtcgtc gaggcactgc
    34501 tcgccgccag tagcggaatc atcgccacca gcgggatcgc cgcaatatcc tggaacagca
    34561 gcaccgcaaa ggcgctgcga cccatttgcg tcaccatcag attgcgttca ttcatcgcct
    34621 gcatggcaat cgccgtagag gagagcgcca gcgtcatgcc gatcaattcc gcgacctgcc
    34681 agcgcagccc aagtaacatg cagaacagcc ccagcaggcc gccgcaaatc accatctgta
    34741 atgcgccacc gccgaacacc gccgcacgca gcttccacag cctttgtgga tcgagttcga
    34801 ggccgataat aaacagcatc agcaccacac caatttcagc aaagtgcaga atagattcgg
    34861 catcggtcac cagccgcaac ccccacgggc caataatgca gcctgcaatc aggtagccaa
    34921 gtactgatcc cagaccgagg cgtaccgcaa tgggtacaat cagcgccgcc gatccgaggt
    34981 agatcagcgc ctgaaccagc gtatgactat ccatgatggg cctcctgcca ttccagcaga
    35041 cgttgcttat agtggcgcgc ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag
    35101 tgcatggcaa acggtggcag ccagttcagc ccgcagtaga ttgccgtcgc ctgtagcggc
    35161 tgcgacagca catcaaagcc cggatgcgca ccaatttcaa aatggctttt cccgccgccg
    35221 gtcgtcaccg cccacagcaa atgtttgcca tgcagcgccg tgccgccgtg accgtaagcc
    35281 cagccgtgcg agaaaacttt atcgatccaa agtttaagga gcggtggaat gctgtaccac
    35341 tgcatcgggt gctgccagac gatcagatcg gcgcgagaca gcgcctcctg ctcggcggca
    35401 atatcgatat tgaagtcagg atagagttga taaagagagc gaatttcgac gccatccagc
    35461 gtccttgcct gttcaagcat ccgtttattc gcatgggaat gatgcggata cggatgcgca
    35521 taaattatga ggatcatagt tattagcccg cgtgtttacg cctttgtttt cccacaaagt
    35581 gtagtcatat attcacgcgg cttatagtca ataatattga ttcagaaaag agagaaatct
    35641 gaacaaatta tcccattctc tgcccgcgga tcgtcatatt gctggtctgc gccagtgaca
    35701 ttcctcgagt ctccggggca aacgctacgg aaatcaacaa gccaaacagc gagatacccg
    35761 cccccattag catcgtgtta ctgataccgt aattattgat aaatatcggc agtgcccagg
    35821 tcgaaacaat ggtgccaata cgacttaagg acataatcac gcccacggca gaggcgcgga
    35881 tatccgtcgg gaagagttca ttaggataga gccactgcaa attacccggc ccgccagaga
    35941 aaaaggcata caccgcaaag gccatcacta ccagccagat ccccatatcc gggattagcc
    36001 ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc
    36061 cggcagtgtt tagccacagc atcggcggaa tacagccgag cataaagaac aggctaatca
    36121 ccacattccc tagtgccgcg tttttgccaa cccccaatcc caacaaacca acgatttgcg
    36181 ggccaaaggt gtaaatggcg aacattggga tcacctggca ggtccagatg gcggcaacaa
    36241 acagaacaaa aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt tgctgcggct
    36301 gctcttcctc gaaagccacc ggttcgccaa acagttttat catcatctct tcgcactctt
    36361 ttactcgccc tttgcgtaac agccagcggg gagattctgg cagttcgaat cgaccaatca
    36421 aaatcaacaa acaagggatc gccgcgctac ccagcatcca gcgccagccg ccttccacat
    36481 cataaagcca gtagccgacc agatcggcgc aggtcgcgcc gacataccac atggcggcga
    36541 taaagctgat ggaaaatgcc cgctgacggg tactggagaa ctcggtgatc attgaggtgg
    36601 cgatgggata atctgcaccg atgacaatgc cgataagtac ccgcatcacc aacagttcga
    36661 cgggggatga aacaaacatc gtcgccaccg atatcacgcc gatggcgatg atatcaatga
    36721 ggaacatttt gcgccgtccg actttatcgg aaatataacc aaacagcgat gtgccaacga
    36781 acagcccggc gagcgttccc gcgcccagca agccaatcca gtcagcgtcc agtttcagcg
    36841 ccggcgtcag ttgctccagc gccacgccaa tcattaccag tacataacca tccagaaacg
    36901 gaccaccgct tccccacagc aaaatgcggc ggtgaataga ggagaatttg agatcgtcaa
    36961 agtttctgga cggttgcatg ggttattcct gttttttgca atgagagtca gtgcggcgtt
    37021 acgtatcaaa ccaacatcag ccgtaacgga actccacacc aaaggtgccg cgcgggtatt
    37081 cccactgttc cagcgccgac cccagcccca gaatgcgaca ggtgccgcac tccagacatc
    37141 cggcgtaatc gaagcgcaca ctgccgtcat cctgcttctt gtacagacct gcggggcacg
    37201 ctttcaccag cagctccagg gcctgtttat cggcatcggc cttcacaaca atgtgcggat
    37261 gctcttcatc gacattgaat ttattgacgc ccagtttgac gtccacattg acgggagaag
    37321 tcataaaacg gtcactcctt tcatgccatc cttgattaga ttgatgaagc ccactttctt
    37381 accgtggcgg aggattttct tgcgcatcag ttctggcgcg ctgccgtcaa tggtgaacag
    37441 gtcacgcgcc acgcccaccg ccagttccgg gtagccgcta aacatgcgtg ggttatcaag
    37501 gaacgccggt agtttctggt acatacgcat atcgcgcagc gggccactct caagatgctg
    37561 acgatattcc gcgagttttt gcttactgaa atcatcgctt ttcatcgctg aaagcacggt
    37621 ttttgctgca gcttccccgg cggcaatcgc cagatccata ccgcgaatgg taaaaccgag
    37681 gttcatacac attccggcgg catcaccggc aatcaatacg ccgtcaccga ccagctccgg
    37741 cagcatgttg atgcctgctt ccggcactac gtgagcggaa tattccacca gcttgccacc
    37801 cgcgatcagc ggtgcaacgg ccggatgttg tttgaaatct tccagcattt gcggcaccga
    37861 ttttttcgcg tcatgcagat gatgcagacc acaaaccagc cccagcgaca gggtgttttc
    37921 attggtataa agaaaaccgc cgcccatcag gccatcagta ggtgatcccg caaacaggca
    37981 agccgcgccc tgattaccct gcaactgaaa acggtcttcg attaccgact tcggtaactc
    38041 gatcagttcc ttcacgccaa ccgccacatc cgtcggtttg acgcgttttg ccatccccaa
    38101 tttttcggca aggatggagt tcaccccatc agcaaggatc accgttttcg cttcaatcac
    38161 atcgccatcg gcttctacac cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg
    38221 gatcccggta attaactgcg cacccgcttc ttcggcctgc tccatcaacc aggcatcaaa
    38281 tttactgcgc aaaacggagt aagaacgctg ggatggcgag gtttcgtcac cgttgcagta
    38341 gtccatagtc atcgctgact tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg
    38401 ttctacgggg gcggagtcgg cgaaacctgg aataatgtgt tccagactgt gggcatagag
    38461 acgcccgccg gtgacgttct tggcacctgc ggaattgcca cgctcgataa ctaacacttg
    38521 cgcaccttcg cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg caccgacgat
    38581 gatggcgtca aagatatctt cggacataag agctccctgt cagcggcagg gccgtaatgc
    38641 agccctgcca gagtggatca acgcgctaaa gctgcggtca gcgccggaag gatcttcacg
    38701 gcgtcgccaa caatgccgta atccgcgtac tggaagatcg gcgcattttt atctttgttg
    38761 atggcgaaaa tggtttgcga tgcattagca ccaaccatgt gctggatctg cccggagatc
    38821 cccaccgcca ggtacagttc aggtttcagc atcaggttgg agataccgac atagcgttcg
    38881 tgctccatcc atttttcgtt ttccgccacc ggacgagaac aggccaactc cgcacctatc
    38941 gccttacaaa gctgttctgc cagcgcaatg ttctctttgc tgccaatgcc gcgaccgacg
    39001 ctgaccacca gacgggcttt gtcgagatcg acgctgttgc tctggcgcgc ctgggttgcc
    39061 gtgcgggtaa tcgccacagc cggagcctgc cactccacgg tgtgcgtttc gccagtgcgt
    39121 gacgcgtctg gctgagccgc atcgaacgtg ccactgctga tggtcagtac cgcatacggc
    39181 gtggcaatgc gttcttcgcc aatcgccagg ccgccgtaaa ccatgtgttt cactgttgct
    39241 ttaccgtcct gtacgctgac ggtgctggca tcgttagaca ccgccgcttt aaggcgataa
    39301 cccagttttg ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac caggccgtct
    39361 gtgccgtgct ggcgaatagt gtcagccatg acactggcgt aatcttcgat catccgatcg
    39421 tccggtttgc cgtttaattt ccagacatga ttagcgccga gctggattgc ctgtgcgccg
    39481 tcggcatcat tgaggacaaa ggtgttgatt tgattagcta aagcctgcgc accgttcatc
    39541 agttccggca gacgagaagg ggtatcgctg aatacccaga cttgagaaaa cgtgttcata
    39601 gcatcccctg taattaaatg actttgcgaa gattttcagc aaatgcggcg atctgttctt
    39661 cgccgtcgcc ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg gcaacctgtt
    39721 gttctgacca ggctgcctct gcgttaaaac cgatatccgc cgccgaccat acctggactg
    39781 gctttttcgc cgcgccgaga atggctttca tcgaaggaat ttgtggggag ttgatatcag
    39841 tggaaacagc aacaaccgca ggcagcggaa tgcttaaggt ttcggtttca tcttccagtt
    39901 cgcgctcaac ggtgagggta tctgccgtca gggagataat tttgctgacg ccgttaactg
    39961 ccggaatatt gaggatttcg cccaccagca gaccaacctg ctgggcataa aggtcggaag
    40021 aaccatcgcc acagaggatc agatcaaagc ctgctttctg ggcggctgca gccagtgcgc
    40081 tcgccgtttg ttgcggcagt gcctgctcga actggtcatc aatcaccaca atcagttcat
    40141 ccgggccgcg cgatagcaca tctttacgcc ctttggcgtt ggtcagggct ttaccgccca
    40201 cacttaaggc tgtcacctgc gcctctgctg cctgttgctt tagctggcaa gccgcttcaa
    40261 tagcgttgag atcgtattgg cttattttgg catcggcttt gctgaagtct aatgaaccat
    40321 cagcattatt gaccgcaata tcctgttcat caggcacgca cttatagcaa gtaataatct
    40381 tcattgcatc tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg ttaattacag
    40441 atatcacgcc ctttaatttg gaaataaaac tttaatcacc aatattgaaa atgtcac
//
LOCUS       DAZGBC010000022        39399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGBC010000022 DAZGBC010000000
VERSION     DAZGBC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.022"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHK1J_001211"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHK1J_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO7910514.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHK1J_001212"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHK1J_001212"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO7910515.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHK1J_001213"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHK1J_001213"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO7910516.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNTGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHK1J_001214"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHK1J_001214"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO7910517.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHK1J_001215"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHK1J_001215"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO7910518.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACHK1J_001216"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACHK1J_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO7910519.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11351
                     /gene="ftsK"
                     /locus_tag="ACHK1J_001217"
     CDS             7245..11351
                     /gene="ftsK"
                     /locus_tag="ACHK1J_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO7910520.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     DITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11506..12117
                     /gene="lolA"
                     /locus_tag="ACHK1J_001218"
     CDS             11506..12117
                     /gene="lolA"
                     /locus_tag="ACHK1J_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO7910521.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12128..13471
                     /gene="rarA"
                     /locus_tag="ACHK1J_001219"
                     /gene_synonym="mgsA"
     CDS             12128..13471
                     /gene="rarA"
                     /locus_tag="ACHK1J_001219"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO7910522.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIILPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13562..14854
                     /gene="serS"
                     /locus_tag="ACHK1J_001220"
     CDS             13562..14854
                     /gene="serS"
                     /locus_tag="ACHK1J_001220"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO7910523.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15095..15235)
                     /locus_tag="ACHK1J_001221"
     CDS             complement(15095..15235)
                     /locus_tag="ACHK1J_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7910524.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(15394..15684)
                     /locus_tag="ACHK1J_001222"
     CDS             complement(15394..15684)
                     /locus_tag="ACHK1J_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910525.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSDDE
                     NEKIRKARTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKTATSSVARNGRKR"
     gene            complement(16100..17773)
                     /locus_tag="ACHK1J_001223"
     CDS             complement(16100..17773)
                     /locus_tag="ACHK1J_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910526.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEAPTEAVSAGN
                     IADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFPPPGTLVEVG
                     FVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWERQTDQRIQET
                     SRERVVSADEEKRSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGHMM
                     VVAGTCERDVEGDTQDTVGGSLTEKIKGVRHSIALAQKLVGDTVKLGNEGINVLTMLT
                     DLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            complement(17776..18318)
                     /locus_tag="ACHK1J_001224"
     CDS             complement(17776..18318)
                     /locus_tag="ACHK1J_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910527.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARAAPASSRKQTPQTPGAKGKTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            complement(18290..19015)
                     /locus_tag="ACHK1J_001225"
     CDS             complement(18290..19015)
                     /locus_tag="ACHK1J_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910528.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHFSVATMRNAVSAARAAQARQLAAQRPDAAGLRRQIS
                     RFISRRAGALAGLEEGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTTRP"
     gene            complement(19027..19623)
                     /locus_tag="ACHK1J_001226"
     CDS             complement(19027..19623)
                     /locus_tag="ACHK1J_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7910529.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWHLMEDHRGKTVWRLDSGAALFISGPGPLPENVTEIKP
                     DGEFCRWDGSSWVKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRTDTGNAPDIVWPESPSPDHHAG"
     gene            complement(19623..21683)
                     /locus_tag="ACHK1J_001227"
     CDS             complement(19623..21683)
                     /locus_tag="ACHK1J_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7910530.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQATGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLASMDE
                     RQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLRAELSTHHPMTTPAFPSR
                     IWLNVWWTGTLTSEWATEHEIITADPDVTEERLRKTYPRDGIQQYFCAIAEIDEDGNV
                     TDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGYVGKAGQRWTTEQFVEWL
                     NERGAFRPGLWIFKANISLSQHSVLTDTGYGDICLGGCLIEVSGDINGGLIRITTGIE
                     SPEDITRPHRTQFTGVILSKGKSAIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPAGYAIMVGQTFDKARYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTDLGTKTTSSFNYGTKTTNSAGPFNATISLRTATASPEGSGDWTA
                     YGPGSGANLIARVSEHTHSVSIGEHIHTLALGQHGHGLTINPTGNAENTVKNIAFNYI
                     VRLA"
     gene            complement(21706..22254)
                     /locus_tag="ACHK1J_001228"
     CDS             complement(21706..22254)
                     /locus_tag="ACHK1J_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7910531.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAAQAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTEIYRRRISYAFINAQEAGEVAGFIAIFE
                     RLGIGYVELLERQPGVDWDVILVQVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     NPFIIHYGAYEGEYICWTARAE"
     gene            complement(22247..23431)
                     /locus_tag="ACHK1J_001229"
     CDS             complement(22247..23431)
                     /locus_tag="ACHK1J_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7910532.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     SGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMTVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYI
                     NEEGHHGHGDDLQCFAMPETRHRLDVTVYIRSSTTLPPDGAEGLKQDIGDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            complement(23409..23759)
                     /locus_tag="ACHK1J_001230"
     CDS             complement(23409..23759)
                     /locus_tag="ACHK1J_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFO7910533.1"
                     /translation="MNTPSLYIDLLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            complement(23756..25867)
                     /locus_tag="ACHK1J_001231"
     CDS             complement(23756..25867)
                     /locus_tag="ACHK1J_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7910534.1"
                     /translation="MTGFAERSQGAFARIGVGVAGLWGVAEGIRAAMDPAWEMQKALG
                     ELEAKGVGAQALTALTTQAMKLGTAFGQSSTEIIRSAWHIRGALDGLSDGDLPRVTAA
                     TSTLAVAARVSSEEASGYLAAMGARFRSEFSAMGAVGFAEDLAGKTAWMVQQFGVEMS
                     TMQELIEGTKNAGADFGVSMAEQFAILGTLSRTMGTEASGIYEEFLRSAPAAAQKMGM
                     SFVDATGKILPMGDILDRLQAKFGRNIEGNVAAQQALDDAFGSGADVIKKLWGQQEML
                     NRSISALGRNDGMKRATEMAEAMTDPWDRLVASFYNIRAAIGNALYPILAPLAGRFAD
                     IGATFVRWLNMFPNIARWLGYITLTMLALGAAGAVTNIVLGVFSFIMEGHKAIIKGAR
                     AAWSLYTLALTKVRSAIIATSLAARLAGVSFLTMSAPVLLVAAAIAALVVVAIKFWQP
                     IKAFIRGFIDGFTSALDAMSPVTAAFGDIARALAPVWQGIKTLFSAFSDLFAPVQLTT
                     GQMNSVTRAGETCGRVVAAAVSMISPALEVVISVIRTLIDIFAIVIRGWQDVVAAFDP
                     TAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTWNWLAEKLNKIPGISIDMKPTEQVNT
                     MTRGATGSTVPPQIAETAVPALAGNRISAELPPGGIRNQIRTDTSTRIDNSRTFGNLT
                     INTQNPMTPGQLDEWTELYAG"
     gene            25941..26168
                     /locus_tag="ACHK1J_001232"
     CDS             25941..26168
                     /locus_tag="ACHK1J_001232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910535.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            complement(26132..26407)
                     /locus_tag="ACHK1J_001233"
     CDS             complement(26132..26407)
                     /locus_tag="ACHK1J_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFO7910536.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQIVEKINEVYSPKLEIELKN"
     gene            complement(26507..26893)
                     /locus_tag="ACHK1J_001234"
     CDS             complement(26507..26893)
                     /locus_tag="ACHK1J_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910537.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDGR"
     gene            complement(26890..27228)
                     /locus_tag="ACHK1J_001235"
     CDS             complement(26890..27228)
                     /locus_tag="ACHK1J_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO7910538.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGKYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGMK"
     gene            complement(27228..27980)
                     /locus_tag="ACHK1J_001236"
     CDS             complement(27228..27980)
                     /locus_tag="ACHK1J_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7910539.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRGSRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            complement(27970..28275)
                     /locus_tag="ACHK1J_001237"
     CDS             complement(27970..28275)
                     /locus_tag="ACHK1J_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO7910540.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            complement(28280..28741)
                     /locus_tag="ACHK1J_001238"
     CDS             complement(28280..28741)
                     /locus_tag="ACHK1J_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFO7910541.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            complement(28743..29888)
                     /locus_tag="ACHK1J_001239"
     CDS             complement(28743..29888)
                     /locus_tag="ACHK1J_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFO7910542.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            complement(29900..30610)
                     /locus_tag="ACHK1J_001240"
     CDS             complement(29900..30610)
                     /locus_tag="ACHK1J_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910543.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQSGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITVMEDQIIAGPRQQKARTWTITLPSRVFLGVTNEEFSHILA
                     RQMNAMGYGLDVDAQDIR"
     gene            complement(30607..31080)
                     /locus_tag="ACHK1J_001241"
     CDS             complement(30607..31080)
                     /locus_tag="ACHK1J_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7910544.1"
                     /translation="MNQLESLTAFITENLPSDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            complement(31077..31550)
                     /locus_tag="ACHK1J_001242"
     CDS             complement(31077..31550)
                     /locus_tag="ACHK1J_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO7910545.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVAEVNTTLAPVVVHWKAQGHERAADVPGARLGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            complement(31658..32356)
                     /gene="gpM"
                     /locus_tag="ACHK1J_001243"
     CDS             complement(31658..32356)
                     /gene="gpM"
                     /locus_tag="ACHK1J_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO7910546.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYITDGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTSWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNAA"
     gene            complement(32374..33393)
                     /locus_tag="ACHK1J_001244"
     CDS             complement(32374..33393)
                     /locus_tag="ACHK1J_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO7910547.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGSENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEAV
                     PGVGG"
     gene            complement(33405..34454)
                     /locus_tag="ACHK1J_001245"
     CDS             complement(33405..34454)
                     /locus_tag="ACHK1J_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO7910548.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     LLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTSPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAATGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTAAKA
                     KLEDAIKAFSTITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGITPR
                     PAGTPAESEKPFDFV"
     gene            34621..36426
                     /locus_tag="ACHK1J_001246"
     CDS             34621..36426
                     /locus_tag="ACHK1J_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO7910549.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAVELGIPPRTVYHWCDVGR
                     WAALLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGHEDGVAAGICGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIIDFAQ
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPTRRAVEFPKESAMRVGCECPDGIW
                     RYIIRLEEAVAGGLSARVDIERIRNLYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEV
                     EMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQ
                     GFNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNR
                     LVMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDV
                     TGHADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
     gene            36423..37475
                     /locus_tag="ACHK1J_001247"
     CDS             36423..37475
                     /locus_tag="ACHK1J_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7910550.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLCF
                     DVDAGHVAEEKAVPVG"
     gene            37513..37776
                     /locus_tag="ACHK1J_001248"
     CDS             37513..37776
                     /locus_tag="ACHK1J_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO7910551.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(37842..38168)
                     /locus_tag="ACHK1J_001249"
     CDS             complement(37842..38168)
                     /locus_tag="ACHK1J_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO7910552.1"
                     /translation="MKTPGMTGKEIILRYLETHTRFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAACR"
     gene            complement(38165..38335)
                     /locus_tag="ACHK1J_001250"
     CDS             complement(38165..38335)
                     /locus_tag="ACHK1J_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910553.1"
                     /translation="MNDTRRISDRELTKMIESARQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            complement(38384..38788)
                     /locus_tag="ACHK1J_001251"
     CDS             complement(38384..38788)
                     /locus_tag="ACHK1J_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910554.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLVTINRKAAIARYVHSAAGRQSRR"
     gene            complement(38785..>39399)
                     /locus_tag="ACHK1J_001252"
     CDS             complement(38785..>39399)
                     /locus_tag="ACHK1J_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7910555.1"
                     /translation="YGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTDE
                     GFDVECGFAAPWTRGNNCPPDQNLTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTP
                     TERKQLTRQLKNPPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALGVQ
                     LHESAVVSVRHGARVRLADGRTAYWNDSTRRIDITPAPNTEAPQ"
BASE COUNT         8259 a        10804 c        11099 g         9237 t
ORIGIN      
        1 ttgttgcctg acctcatggg gggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattacccc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattc agataatccc
      841 gacggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gcccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atcaccgcca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttctc cggtgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaacgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cctgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccag gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactatc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gtcgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca tcccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgctcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tgtcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaactccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctg aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatccggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcctaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg aatatgaaga tgacgaagag tatgaagatg
     7921 aaaaccacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gatgctgcgc
     8041 tgttctccgg taagcggatg gatgatgaag aagatattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaat ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgcaa ctgcgccgga acagcctgcg caacagccgt
     8581 attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc
     8641 aatccacttt tgctccacag tctacatacc agactgagca aacttatcag cagccagccg
     8701 ctcaggagcc gttgtaccaa cagccgcaac ctgttgaaca gcagcctgtt gtggagcctg
     8761 aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactacttt gaagaagtgg
     8821 aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac
     8881 cggttaaaga accagaaccg atcaaatctt cgctgaaagc accttctgtt gcagcagtac
     8941 ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac
     9001 tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg
     9061 gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc
     9121 gcgtgccaac tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca cagcgtgcgg
     9181 cggaagaaaa agcccgtgaa gcccagcgca atcagtacga ttctggcgat cagtacaacg
     9241 atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc
     9301 agcaacgcta tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg
     9361 ctgcggcaga ggctgaactg gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg
     9421 gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga
     9481 aagcgttgct ggatgatggt ccacacgaac cgttgtttac gccaattgtt gaacctgtac
     9541 agcagccgca acaaccggtt gcaccgcagc cgcagtatca gcagccgcaa cagccggttg
     9601 caccgcagca gcagtatcag cagccacaac agccggttgc gccacagcag caatatcagc
     9661 agccgcaaca gccggttgca ccgcagccgc agtatcaaca gccgcaacaa ccagttgcgc
     9721 cgcagccgca gtatcaacag ccgcaacaac cggttgcacc gcagccgcag tatcagcagc
     9781 cacaacagcc agttgcgcca cagccgcagg ataccctgct tcatccgctg ttgatgcgta
     9841 atggcgacag ccgtccgttg cacaaaccga cgacgccgct gccttctctg gatttgctga
     9901 caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggcgcgtc
     9961 tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgtcaat tactctccgg
    10021 ggccagttat cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt
    10081 ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag
    10141 ttattcctgg caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt
    10201 atctgcgcga agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg
    10261 tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact
    10321 tgttggttgc ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga
    10381 gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc
    10441 tggagctttc ggtttatgaa ggcattccgc acctgttaac ggaagtcgtt actgatatga
    10501 aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgg tataaactga
    10561 tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc
    10621 gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc
    10681 atccggtgct gaaaaaagaa ccatacattg tcgtgttggt tgacgaattt gccgacctga
    10741 tgatgacggt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg
    10801 ccgcgggtat ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc
    10861 tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac
    10921 gtaccattct tgatcaggct ggcgcggaat cactgctggg tatgggggat atgctctact
    10981 ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag
    11041 ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca
    11101 cctccgacag cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc
    11161 cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg
    11221 tacaacgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag
    11281 cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc
    11341 cgtttgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat
    11401 caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag
    11461 tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgcc
    11521 atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc
    11581 gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg
    11641 actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca
    11701 aacttattca actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa
    11761 acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc
    11821 accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac
    11881 aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg
    11941 aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag
    12001 caggacgatc agcgcagcag ttatcaactg aaatcccagc aaaatggggc tgtggatgca
    12061 gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg
    12121 cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc
    12181 gcgtatgcgg ccagaaaatt tagcacagta tatcggtcag caacatttgc tggctgcggg
    12241 gaagccgttg ccgcgcgcta tcgaagccgg gcatttacat tctatgatcc tctgggggcc
    12301 accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt
    12361 ggaacgtatt tctgccgtca cctctggtgt gaaagagatt cgcgaggcga tcgagcgcgc
    12421 ccggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt
    12481 caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat
    12541 tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg
    12601 tgtttatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat
    12661 ggaagacaaa acccgtggct atggtggtca ggatattatt ctgccagatg aaacacgacg
    12721 cgccattgct gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat
    12781 ggcggatatg gccgaagtcg atgatagcgg taagcgggtc ctgaagcctg aattactgac
    12841 cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct
    12901 gatttccgca ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta
    12961 tgcgcgaatt attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat
    13021 tgcgtctgaa gacgtcggta atgccgatcc acgggcgatg caggtggcaa ttgcggcctg
    13081 ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt
    13141 ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc
    13201 cgatgctcgc gaacgcccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa
    13261 attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc
    13321 ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc
    13381 gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg ctgaacagga
    13441 tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt
    13501 atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag
    13561 catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg
    13621 ccggggcttt aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt
    13681 gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca
    13741 ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga
    13801 agagctggat gcagcaaaag ccgagctgga tgctttacag gctgaaattc gcgatatcgc
    13861 gctgaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa
    13921 cgttgaagtc agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt
    13981 gacgctgggt gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc
    14041 ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat
    14101 gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa
    14161 ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac
    14221 tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt
    14281 tccgctgact aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat
    14341 gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg
    14401 tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccggaaga
    14461 ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg
    14521 cctgccgtac cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac
    14581 ttacgacctg gaagtatgga tcccggcaca gaacacctac cgcgagatct cttcctgctc
    14641 caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgtcgcagca aatcggacaa
    14701 gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt
    14761 tgcggttatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg
    14821 tccgtatatg aacggactgg aatatattgg ctaataccta atttttctga atctaaaaag
    14881 cgcctgcggg tggttttagt gggagattaa catattgaaa ataaagattt tttattttca
    14941 tgtgtccaga taacgtccat ctttgaaaca aaaagcccgc agttttttta cgccgcgggc
    15001 tttgtttttc tttgtgactt ttgtgtgagg gtgcgacttt cgaccgaacc tttggcaacc
    15061 ggatagcggg ggctttcacc cccgctgcgg tttcttacgg tttacactgc aagaacgccg
    15121 caaattctga ccacatgatg ttaagtctga actcacacgg agaacggtgt atcatccaga
    15181 cggtcaacaa catggttaag cataaaatgc tcacgatgat cgttttttgc ggcatagcgc
    15241 ttgactcctt ttacagggag gcgctaacct tccacttgtc tagggtagga agggttaggc
    15301 ctcgggttaa acaaatgttt tactcggggc ctttccacat ccggccttca ggtattcctt
    15361 ccagccgtca gccgaaaggc acccgcgcgt aatttacctt ttacgtccgt tacgtgcaac
    15421 ggatgacgtg gctgtttttc cttccccttc gccatccttt ttcggagcag ttgtgcaaaa
    15481 gtgtgcaaaa gtgatttttg ctgccactcc ccgccaggcc gcgccagttc tggctttcct
    15541 gattttttcg ttttcgtcgt cggatccttt ttcagatctt cgatcgtgac gtaattttaa
    15601 aaatctcttg ttattcagtg cattaaaatt tttcgggatc ctcagaaagc cggagttttt
    15661 gcacactttt gcaaaacctt gcacaaagtg caaaaccatt cagaagggaa aacctcagta
    15721 atggcgcgcc ctggcggggt gttttgcacc catttcgatt tgcaaaattt atcgtacaca
    15781 aataccggag gcgtgtgcgg ggtagcgccg attacgtcac tttacgcgtt acgtcgcccc
    15841 cgaaaatttc cggccatttg cggcgattct ggggcgcaga aatcgttttc tgatgtcagg
    15901 ggatcgaatc gggctggcgg ggttatctct tcgttacgtg gcgttacgtg acgttttacg
    15961 cggggattac gtggggcgtt tcgtccatct ttttttctgc tgcggacggt ttttcgtggt
    16021 gggtgatgtc cgttgatgaa tattttttat cgtccgggaa taaaaaaggc cacattgcgc
    16081 ggcctgttaa agtgaatgat tattcgatta ttggtgaata tttattttta agctggcggc
    16141 actcctgagc gacctggctg aactgtgcgg cctgcggaga cgtgcccgtt tttgggtggg
    16201 tatgtgatgc tgtaatatcg gccagctctt ccaccacatc ggcaaggtcc gtcagcatgg
    16261 tcagtacatt aatgccctcg tttcccagct tcaccgtatc gccaaccagc ttctgcgcca
    16321 gcgcaatact gtgacggacg cccttaatct tttcggtaag gctgccgccc acggtgtcct
    16381 gggtgtcgcc ttccacgtcg cgctcacagg taccggcaac caccatcatg tgcccgcccg
    16441 tgctgatgct gtagttctct tcagtgatat gggtgaccgc gccagcctga agcgtggata
    16501 cgcccaggac ggtggttgtg tcggttgcct gtatggtggt ctcccggctg gtcaggctcc
    16561 gcttttcctc gtctgcgctc accacgcgct cgcgtgatgt ctcctgtatc cgctggtctg
    16621 tctggcgctc ccagtctccg gcgaccgtca cgcgctgcga tacgccgtcg cgctgttgct
    16681 gcaactgctc gcccggcttc acgtcgggca gattgtggcc ctctgccatt atctggcgga
    16741 tgaagggctt atccgcccgg ccttccacaa atcccacttc caccagggtt ccgggtggcg
    16801 ggaactggaa catgccggac tcactgcccg ccatgggaac ggggagggga acggcgttat
    16861 agacgggcgt atctgctgcc gggttgccgt tctcgtcaag cagctggaga ttcaccgcgt
    16921 agcggggtct gaacgggtcg gcaatattgc ccgcgctgac ggcttcggtc ggtgcctcaa
    16981 cacgtgccag tttgggcagg tgaagccctg atgccagctc agggaaggcc ttttcagcct
    17041 ggcgacggaa aggggacttc tggaggggag cgccggtagc tttattgcgc ggtgtccacg
    17101 tgatggtcat gttgtcatct tccagcatga ccatcgtcag gcgctgaccg ttcacctcca
    17161 cacccggtcg cagcgtctga atcatcggga gggtcatact gtgcccgccc gttgatgcct
    17221 ggctgaactc gtgcgggatg gtgacggggc gaccggaaaa catcccctgc gcggcagcag
    17281 cggccataat gccgccgtcc ggtaacggat gccagatgta atccggtacg gagaaaatac
    17341 gccccagact ggccagcagt tcgtagcccg tgccgctgtg tgtgaaatgc ggaacgggtc
    17401 tgtcagtgta agccgcgttg tccggcagga tgatttccag cccgctggtc tcactcagcc
    17461 actgtgtgac cgtgcgcatt gtcgggtgct gaaatgaaca ggggaagggg tgatcaagga
    17521 tccccgccat ttcacgaatc agaagccgca ccgagccgtt ttcagcgggc tggctgcgtt
    17581 caacgtatcc ggtaaaccag cgcaccaggt cgtttccgta tccgatatcg agacgaacgg
    17641 ggcgcccggt gtagttctgc tctgttctgg ccgtgataaa tccgcgtccg cacgcgttca
    17701 gctcaagcat gacggaacaa ctgaccagct gaatggtgtc gttacccagt aacagacgtt
    17761 tagtggggtt catggttact ccagcgcatc attgacgggt ttgaggacct tttcttcgaa
    17821 ccaggtcatt ttctccggtg tttcgtcggc cacggttttg ccttttgcgc cgggcgtctg
    17881 cggtgtctgt ttacggctgc tggcgggtgc ggcgcgtgcc tggcgttttt ccggtacgct
    17941 tcgtctttcg cgtaatgtga agctgacctg ccaggcgagg cggtcggtct gctgctgtgc
    18001 gtctatctgc ccggaaaatg tcccttcacg aaagttaatg gcggcagcgg tggcgtttgc
    18061 gatacggtaa actttcaggg caccgtcccc ggtggtggca gaggccagct ggaaaatgcg
    18121 ctgaagggcc cgctcattgt caaaggtgat catcccggtt acgcgaagct ctttggcctt
    18181 gatgccctgc tcggcgttcg cggtgctcga tgtctgaccg gactggtcct tttcctggaa
    18241 ctgcatcgag ggcgaaatca ccatgttctg catgaaaata ccctcaccat caagggcgag
    18301 tagtgcggtc tggctcatgg atcattttcc ccagttcggt taatgagtct gccgccagga
    18361 gtgcggccac agtatgtaca gcggtcggtg ccgggatatc cttcaccatg gccgccgctg
    18421 cggcgcggtg atatccccgg tactgaaata cccaggcctg cgccttctgt tctgccagtg
    18481 ttctgatccc ctcttccagt ccggcaagtg cgccagcccg tctgctgata aagcggctta
    18541 tctggcggcg aagccctgcg gcatccgggc gttgtgctgc cagctgcctg gcctgtgctg
    18601 cacgggcggc gctgactgcg ttgcgcattg tggcaacgga aaaatgaaca ggtgcgggca
    18661 gggtgtcggt ggtgatcggg atttgcattt tgtcagtgct cagactggcg gcagcctgcg
    18721 ccatgcgctg tgcctgcgtg aataccggca cgggcagaac ggcggtcagt gccgccattg
    18781 actgtataaa aagatcgtgg gtgttttcgg caatcatgat aatggtcacg tcgccggatg
    18841 cacggcttcc ggccattttc ccggccagcc agtttatggc gttcggaggg cttaaatata
    18901 cccccgattc ttcatatcgt cccagcccgt aagtccacgg tgtgacgggg atgacggaac
    18961 agctcagcgg gggcatgtca gcgctgacgc tgattgtttt tcttgtccac gtcatggtgc
    19021 ctccggtcag cctgcgtgat gatccggcga aggggattca ggccagacaa tatccggcgc
    19081 gttgcccgtg tctgtccggc tcagtaatac ccggtatttt ttccacaggg gaagcagggc
    19141 gctttcatcg tcggtcgcca tatttaaatc cacggcatcc tggaggatgt ttattgtatt
    19201 tgttgcttcc tgtattaatt cccgtttttt attctccgcc tcgctcaccc ggtaagcctg
    19261 ttctgctgct tcatccttaa cccatgatga accatcccac cggcagaact ccccgtccgg
    19321 ttttatttct gtcacgtttt ccggcaacgg tcccggcccg gaaatgaata aggcagcccc
    19381 cgagtccaga cgccagacgg ttttaccccg gtgatcttcc atgaggtgcc aggtgttttc
    19441 gtcctcatca aagatagcca cgtgtccttc aggaatgtca ggcggggcaa tgtccgttga
    19501 gtgcgcaggc agtccggtat gcggggggat ccaggcatcg ccttcgccga taaattcatt
    19561 tgttccggct gacaggttgt agattttaat tgtctgcggg tgttcgctca ttttaaaggt
    19621 cattatgcaa gcctcacgat gtagttaaat gcgatgtttt tgacggtgtt ttccgcatta
    19681 ccggtggggt taatggtcag gccgtgacca tgttgaccca gtgcgagcgt gtggatgtgt
    19741 tcgccaatgc ttaccgaatg tgtgtgttcc gaaactctgg cgatcaggtt cgccccggag
    19801 cccggcccgt atgcggtcca gtcaccgctg ccttccggtg acgcggtggc ggttcttaat
    19861 gatattgttg cgttgaacgg ccccgcgctg tttgttgttt ttgtgccgta attgaaggat
    19921 gaggtcgttt ttgtgccaag gtcagtattt gagacgctcc ctccgtgggt gtgcgatttg
    19981 atgccgtcct gctcctgtga caataccgca cgaccgctgg caggtttccc cttgattgtc
    20041 cagccgcgca tatccgggat gcgaccgtcc ggataggctt ttgccagcag gggatatcgt
    20101 gctttatcga atgtctgccc caccatgatg gcgtaaccgg caggtggggt gtctgacggc
    20161 cacggcaggg gaacacctgc gggtaacaac tcacttgcag gcagtgcgcc gatgtcctgt
    20221 ggtgtgggtt tgtttttgtc cgtgtaagtg cggtaccaga tggcgctttt ccctttactt
    20281 aaaataactc cggtgaactg tgtgcggtga ggtctggtga tgtcttcagg ggattcaatg
    20341 cctgtagtga tcctgatgag ccccccgttg atgtcgcctg atacttcgat aaggcagcct
    20401 ccaaggcaga tatcgccata gcccgtatca gtgagcacgc tgtgctgtga cagtgaaata
    20461 ttggctttga atatccagag tcctggtctg aaggcgccac gttcattcag ccattccacg
    20521 aattgttccg ttgtccagcg ttgccctgct ttacccacgt acccgtcttt tgcataaacg
    20581 cgtggtgcgc ccacgttgat caggaacagc tccttgttgg ggatgtcctc accattgcgg
    20641 tttttcgcaa gccgaccatc ggcgttgtcc atggcgatct ttatagcctt tggcgtggct
    20701 gcctgtgtct ctgatgtact ggtggtggcg ctgcttaact gtgtgaagcc cttttctttc
    20761 agcgttgcgt ccgggtggtt gcgtgatttc tcgtgttttt tcagggcgtc cagactgcgg
    20821 cgctcttccg gggttcctct ggggcggagg tcagtcacgt tgccgtcttc atcaatttca
    20881 gcgatggcac aaaaatattg ctgaatgccg tccctcggat acgttttccg taaacgctct
    20941 tccgtaacgt ccgggtcggc tgttatgatt tcgtgctctg tcgcccattc actggtgagc
    21001 gtgccggtcc accacacatt cagccagata cgggacggga aggcgggcgt ggtcatgggg
    21061 tggtgagtgc tcagctctgc gcgtagcccc tccacgtaac ccaccccttt gttgactgtc
    21121 caggtttcgc ctttcttaac cagccacccg tcatcaaaaa aagttgcctt cccgtagata
    21181 tcgacgtttt cgcggcgctg gcgttcgtcc atggatgcca ggcgtgccgt gaagtctatc
    21241 tgccaggtct gcgccggggt ggtgatacct gtggcttcct tcgcaccgct gtactccatg
    21301 agcatggaac ggaccagcac attaccttcg ttaccgttgt gggttctgat ttttttctgt
    21361 gtgggggcgt gaacaatcat ggccagcgtg ctggttgctt tgctgaccag tcccacccag
    21421 ttaaattcaa aatcacccag ctctgccccc agggtgaccg aataaaccac ggcattctca
    21481 ttcaccacgc cggttttgcc ggtagcctgg cggtgcacaa tctgatcttc aggcggcagg
    21541 gtttcgtcgg gggataccgg tgtttccggg ttgaggtccg ggacgtttgc aaaaataaac
    21601 tcatccagca caacgggggt gccttccgcc gcctgctgtg ccttgagccg gataaaggcg
    21661 ctggtaatta cagatttcgc cataaagtca tactccttca gtcattcact ctgccctggc
    21721 cgtccagcag atatactccc cttcgtatgc gccataatgg atgatgaagg ggttgagggt
    21781 ttccacctca aaacggtaac ggcggcatgt cctgccatat ttgcggatga tctccagaag
    21841 aagatcgctg ttgtcggcta tctggctgtc tgtcacctgc accaggatca catcccagtc
    21901 cacgccgggc tggcgctcga gtaactccac gtagccgatg cccagccgtt caaaaatggc
    21961 gataaagccc gccacctcgc cagcctcctg tgcattgatg aaggcgtaac tgatgcggcg
    22021 gcggtagatc tccgtgggtt ctcctttaaa gcgggtgatg tcccgctccc atgccagtaa
    22081 atcaagtaca ggctctgcac atgtcatggg gtcaaactgg cgcacgggcc aggtgatcca
    22141 ttcccatacg cgggaccaga acgcctgcgc ggcccgcagc agtttgtcgg gttctcccct
    22201 gtccatccat gagggcagtt tcagcgagcg aagccgctgc aaaaagtcat tcaatgacca
    22261 cctccactgt cagggattcg agccgtggca cggccagatc gctgatgatg tcttccagtg
    22321 aaaactgaac cgaagccacc accgggaagg tctgatgaat ttccttgccc agtgtggaaa
    22381 acgaaaagcg cgaatagggc cagacttttt tcacattaaa gcgggcgttc tcccggaaag
    22441 cgcagcgtat caggtcgccg atatcctgct tcagcccttc cgcgccgtcc ggtggcaggg
    22501 tggttgaact tctgatgtat acggtgacgt ccagacggtg acgcgtctcc ggcatggcga
    22561 aacactgcaa atcatccccg tgtccgtggt gcccctcttc gttgatgtag cggttgactg
    22621 cttcaatgaa aggggcagag gcctcgccgc tgtccagtaa caggtaggcg ttggccgttc
    22681 ccggtcctct cggggcgtcg tgctgaaagt agatccggtc gatgctcagg cccgccacgc
    22741 tggcgataag tccccggtag acggcgtccg tgtggtaact gctcacgagg ttaaactggt
    22801 tgcgtattct gtcgcgcagt tcgtcgtcgc tttcctcatc ggcacccggt acggtcagcc
    22861 agtcttcccg gttcaccacg gatgcaatgc cgggaatggc aacgggaagg atacggaagt
    22921 accccggcgc gaggttgtgc ccgctgcctt cggtggtggc ttccacggga acggatgcgc
    22981 cgcttttacc gctgctgata agcgtttctt ccgtgacggt catgctgtat atcacgccgt
    23041 taattctggc ggtctggacg atggtgcctt cggggatgag gacgtcatca tttgccctgg
    23101 ttttggcaaa ccagatcacg ccactggcct tactggccgg ttttggggtg atgttgacgt
    23161 cgtgcgcatg aagcagaaga agtgaccggt cagccgtggc gacaaacata ccgggcagca
    23221 caacgtcaac cagcgcatca cgcagccact gaacgggtcg tatgacaatg gtcagaatga
    23281 gccgccagaa gggactcatg ggcgaggtgt tggtgatgag tccggttttc tgtacatcct
    23341 catgaaagag ggtggcgatc tcttcttctg tttccggcat accgctttca cggaatacct
    23401 gattaaaatc aacgtccggt ttccctttca tagttcaccc tggtgtttat gcgtccaaaa
    23461 tcctgtgtct ctgccgtcac atgcagcctg cccggtgcct cttcgttaat gaacaccgtc
    23521 cccggaacca gccgttcatc atcctcaacc aggaggataa gccgggtaat gatgtcgctg
    23581 cgcagcgtcg ggctgcgttc gccgataagc agggcggcga tcccgctttc gataatgctg
    23641 tgcaccacgt cctggccgat gctgacccgg ttatcacatc gctgtggctc gttgccgctg
    23701 ttcagcgtaa aactgccgtc agtgataagc aggtcgatgt aaagcgatgg cgtgttcatc
    23761 ccgcgtaaag ctccgtccat tcatccagtt gccccggcgt catcgggttc tgcgtgttga
    23821 ttgtcagatt gccgaacgtc cggctgttgt cgatacgggt tgaggtgtcc gtgcgtatct
    23881 ggttgcgtat gcctccgggt ggcagctctg cgctgatgcg gttgcctgcc agtgccggga
    23941 cggcggtttc tgcaatctgc ggaggtacgg tgctgcctgt tgcgccccgc gtcatggtgt
    24001 tcacctgctc cgtgggcttc atgtcgatac tgataccggg gattttgttc agcttttccg
    24061 caagccagtt ccacgtccgg ttaaacgagt ttttaatgac gttccataag tccccgaaga
    24121 cgttaccgat gatccccgcc attttccgga aggattcgac cggggcggtc gggtcaaatg
    24181 cagccaccac gtcctgccac cctctgatga cgatggcgaa gatgtcgatc agggttctga
    24241 tcacgctgat aaccacctcc agcgcggggg atatcatgct taccgctgcg gccaccacgc
    24301 gaccgcatgt ttccccggca cgggtgacgc tgttcatctg tcccgtggtc agctgtaccg
    24361 gtgcaaacag atcggagaag gcactgaaca gtgtttttat gccctgccat accggggcca
    24421 gcgcccgggc aatgtcaccg aacgcggcag tcaccgggct catggcgtcc agtgcgctgg
    24481 taaaaccatc aataaagccg cgaataaagg ctttgatggg ctgccagaac ttaatggcca
    24541 ccaccaccag agcagcgatg gcggcggcaa ccaggaggac cggggcgctc atggtcagaa
    24601 acgacacgcc cgccagacgc gcggcaagac tggtggcaat gatggcactg cgtacttttg
    24661 tgagtgcaag cgtgtacaga ctccaggctg ccctggcgcc ctttatgatg gccttatggc
    24721 cttccatgat gaagctgaac acacccagca cgatgttcgt gaccgcaccg gctgcgccca
    24781 gcgccagcat ggtcagcgtg atgtaaccca gccagcgggc gatgttcggg aacatgttca
    24841 gccagcgcac gaacgtcgcg ccgatatcgg caaaacggcc agccagtggt gcgagaatgg
    24901 ggtagagggc gttaccgatg gcggcccgga tgttgtagaa ggaggccaca aggcggtccc
    24961 acgggtcagt catggcttcc gccatttccg tggcacgctt catgccgtca ttacgcccca
    25021 gcgctgatat gctccggtta agcatttcct gctgtcccca gagtttttta atcacgtccg
    25081 cgccactgcc gaaggcgtca tccagcgcct gttgtgccgc cacgttaccc tcaatgttcc
    25141 tgccgaactt cgcctgcaga cggtcgagga tgtcacccat gggtaaaatc ttcccggtgg
    25201 catcgacaaa actcatcccc attttttgtg cggcagcggg ggcactgcgc aggaactcct
    25261 catagatgcc cgatgcctcg gtacccatgg tccgtgacag ggtgcccagt atggcaaact
    25321 gctctgccat gctgacgcca aaatccgcgc ccgcgttttt ggtgccctca ataagctcct
    25381 gcatggtgct catttccacg ccgaactgct gaaccatcca cgcggtttta cctgccagat
    25441 cttcagcaaa ccccacggca cccatggcag aaaactcact gcggaaacgg gcacccatgg
    25501 cggcaaggta gccgctcgcc tcttcgctgc tgacgcgggc agccacggcc agggtgctgg
    25561 ttgctgccgt cacacggggt aaatccccgt cgctcagtcc gtccagtgcg ccgcgaatat
    25621 gccatgcgga acggatgatc tccgtggaag actgaccgaa tgcggtcccc agtttcatgg
    25681 cctgcgtggt gagggcggtc agcgcctgcg ccccgacgcc tttggcctca agctcgccca
    25741 gcgccttctg catctcccac gccggatcca tcgcggcgcg gataccttcg gccacgcccc
    25801 acagtccggc cacacccaca ccgatacgcg cgaacgcgcc ctgcgagcgt tcggcgaatc
    25861 ccgtcacgga ttcctgaacc tgccgcagcg ggcgggtaag ctggtctgtc agattgataa
    25921 tgaaatccag ctggctggcc atgtcacctt ccgttaaatg cgatcgctat accttcagcg
    25981 gtcttgttgg cgcgggtacg ggcaaaatat tcatccagcc agatggcacg ggcgatgctc
    26041 tcttcgtcgt ccggctctcc ggggaggtag tgacgccgca atgccagata ctgctcaagg
    26101 ggattgcggc ggatgctttc gacccgcgcg gtcagttttt taactcaatc tccagtttcg
    26161 gggagtaaac ctcgttaatc ttctcgacga tctgcatctc acagcccgga taacgcgcaa
    26221 tcaggtcagc cagtgcgtcg cggctggcag gcgtcacgat gcggttaaga taggtgctca
    26281 tcggcgccac cttgttggtc atcgtgattt cgttgatgag gttgttaaat gccgttttgt
    26341 tcggttcaaa gctcagggtt acgcccgcaa cggacaggat gatggtgccg gtgttcttcg
    26401 tggtcattgc agtttctccg tgtttcgtat ggtttccacc aggctgttat gcctggcggc
    26461 gcattcggtg tagcggctcg tgagggttcg cagtgccgca tcaaaatcag cgcccgtcgg
    26521 gcctgccagt ctcggcagtg tcttcgggca ttccgtcagc tggttttcct gccagggtac
    26581 gttcagcggc ttcagcacgg gcgttgaaca gccggacgta atcatcagta gcgcacacat
    26641 tgcggaatac cgggcggacg gtttccgtgt gaataacgcg ttcagtgtga atttcattgc
    26701 cctttaacgc ctccagccgt ttttccagta tgcgggcgga ctgctgcccg gcttctgtta
    26761 ccgcacgtcg cgtggcttcc atggcttcct gttcagcaag cgccaccctg gcgtcatgcc
    26821 agtcatgggc cagccagccc gcccacatgg cggcaaagag gatcaccagg cagcccgcca
    26881 gctgtttcat catttcatcc cgttgtgctc aaggctgaaa tgattgccgt ccgcccgact
    26941 gaagcgcccg ccccatgtgc cgccgatgga ttcccagtat tcccccagcg gcctgtaggc
    27001 gtcggtgtcg gcctggtatt tgccgttaat gaacaggttg aagtccaccg cgaggcgcag
    27061 cgtgtgaagg ctgttacgaa tgccttttcc gcttttcgca ttcagtgcgg cctgctccgg
    27121 cgtgcggtat gcctcgccga aggtcaggcg atagccgtgc tcctgtgccc agtggatgag
    27181 atcggcaatc atgacggcaa acagttgttg tttttcgctc agtttcatca gtcattcctc
    27241 tgttccgtcc ccgtaaggcg cgcctgtgcc acgctgaaat agtgggcgtt cagttcaata
    27301 cccgtaaaac ggcagccccg gcgaagggcg gccacgcccg tggtgccgga ccccataaac
    27361 ggatcaagga tcagccctcc gggttcacac acgcgcacaa gctcatccat caacgattcc
    27421 ggtttacctg tcgggtgctg tttctcctgt gggcgtgtgt ggtgcgtgat gcagccgtcg
    27481 aacggccctg aaggggattt gcccagtgcg cctttgctgc cccagaccac atattcgcac
    27541 tggtgacgaa aataaccggt atgtggtgtt cttgagcccc gtcccttgtt ccatgccacg
    27601 atcccccgcc aggtaaaacc ggcggcctgg aatgcgtccg tcagcgcggg caactgccgc
    27661 cagtcggtga agaccagggc gtaaccaccg ggtcgtaccg tgcgccatgc ctgtgtcatc
    27721 cacagatgcg tccagtattc ccatgaacgg gcgtccatgt tctcgccttc gaagccttca
    27781 aagcggtgaa gatcttcatc gttgatgtat ttggcgtggc tgccctggca tgtccgcccc
    27841 gccttgtgtg tggcacctga actgtatggc gggtctgtga tgagcgcgtc cacactgttt
    27901 tccggcagca tccgcagaac gtcatgcgcc tgcgcgtgat acaggctcag ggaaggcgaa
    27961 atgtaaggct tatgggtcat ttctgcgttc cttctgcttc aggtagcggt ctgccagacg
    28021 gcaaaagcat tcttcaataa aggcgctgcc cataatgccc agcgcgcacg ccagaccgac
    28081 cactaccagc tccggcatgt ccggaaattt aaacagcgga atggcggcaa ccggtgccac
    28141 ggcagagccc ataatcattc gcccgataag cagacgggct gtgatgcgat cgcggctcac
    28201 catgagacgc cccagcccga tggcagcacc gatgataatc agttttgcca ggagtgaagt
    28261 ctgaccgtcc ggcatggcat caccccttca gatcgcgggt gtcacgggct gacaggtagg
    28321 gcacgccgtc aatgctgata aagtccgggc ttgtgaccat gaacttgatt tttttcgtgg
    28381 tctttccggc ttcacctgcg ctgatgttca ccacgtccga caacatgggg acgcagccga
    28441 acacctcgat tttttcctcc tcgctgccgt tgttggcata aaagagaaaa tccttttcag
    28501 gaatagcacg ccacgacccc gcagagcggg ccacggcacc aaacttttta aagttctgcg
    28561 cgtcgacttc cacctccaca tcggcagaaa cagaaccacg ggtgtagccg ttcggcacgc
    28621 cgcgcgtctg tgccacggcg ctgttgtcgg tgatggtgac ggtggcgctt tccacgtgga
    28681 tcatgatccc gtcgtagttg atatcaaacg cgccgccgct gatacgttca tttccggcca
    28741 tgtcagatct ccagtgctgt gtcgagttca atacttacgc caatggcttt tgcgctctcg
    28801 taggggcgta ccacaatgta aatctgcacg tgtgtactgc tggtccagac gatggtgata
    28861 tccccgtcct gtggcggttt aacctcgccg gggaacggta cgccgttaat ctccgtggcg
    28921 agcgccattt cacgcagcgt gcggctgaaa tatgtctggc tggcggcaat gctgcccggt
    28981 gtgctgttga gtgcgcgatc cgcaaggcgg gcaatggcgc gcagacgtac acggcgggag
    29041 actttatcca ccacgcgcac gttttcgatg acctggtaat ctcccccctc cacatcaagg
    29101 gtgcgtccgt ctgaccagta gagcccgtcg aaatcgtgat accacatggg cacgctgaag
    29161 cgtttgcctt ccagcgagcg cagcgtggcc agctcaaggg gctgtttctt gccgtcctgc
    29221 gggatctcat cgttacccat cgccaccagg gcaccggttg ccacacgtgc cggactgtcc
    29281 gcaacggtga cggaaggatt acagagacgg cccgccagta cgccgggctc atttccccat
    29341 aagcggggaa ccaccatcac gccaggtgct gccacaccgt cctgtatggc actgatgcgg
    29401 gtgacgtatg ccgcccatgc ctcatccttg cccggtccgg ccacgcacag catgaaccag
    29461 acaaaacggc cataagcgga ctgaagcagg gcgcgggttt cggtggcgcg gttaacggca
    29521 tctttggccg tgatgtcaat ggcaatcacc acgccctcgg cagaagcaat ggtctgcgct
    29581 tttttcaccg cgtccaccca tgcggcgtta gcgtcgccgc cctccggtgc cgggggtttg
    29641 ggttcagaca gaacgtgaac ataggcaaac cagttctggc caccgttggc gattgcggcg
    29701 gtaagcgtgg atttaagcag gctttcatcc ggtcccagca cgtcgtccag gtcggtctca
    29761 ggggagacgg aaagggtgtt acctgtgttc tttttgccat agcccacaaa aagcagcgtg
    29821 cgctccactt cacgggtggc accgttgaaa cggttctgat ggcttacggt tacattcggc
    29881 cagctcacgg tgtgactcct tatctgatat cctgtgcgtc aacgtccagg ccgtagccca
    29941 ttgcgttcat ctggcgtgcc aggatgtgtg aaaattcttc gtttgttacc cccagaaaga
    30001 cgcgcgacgg aagggtaatg gtccatgtcc gggccttctg ctgacgtggt ccggcgataa
    30061 tctgatcttc cataacggtg atcagcaacc cggcctgcgc ccgcgaaagc gttaacaggt
    30121 atttcatgga tgctttccgc cagcgcccct tctgacgtac cttaaagccc agctcccgca
    30181 gtcgttttgc ctgtcggcgg gtggcggtct gtccgctctg gtcctgaccg ctgaagctgt
    30241 cagcactgac ccggatttct gcgccgttgt ggtgaaccca gcccacggca ccggcactga
    30301 tgcggctcct gccactggtg tgattgccgt tacggaaata aacctttacg gcatcctgtc
    30361 cgggcatctc acggaccgcc agaagacgcg gcagctttct gaacattttc cccttaccgc
    30421 gtttcctggg tgcccatgct gtaccgtccg gtgcggtctg atcgcgctga tggcgtctgg
    30481 ctgcggcgat aatgccgtgc cgggcaatac gccataacag gcgacggcgt tttttcgggg
    30541 gcagatccag cgactgaagc atcgcctgcg cttctgccag ctgctgcggg ttcagctggc
    30601 cgcttatcat tcgcggctcc tgctgacaaa cagatccatg gtttccgccg tccagatttc
    30661 agggttaacc agtgtccagc gtttccccct gtacggaacg gggccgtcct cctgcacccg
    30721 gatgatcagc gggtcagcca gtcccaccgt gatatcgaga aggcacgagt cctcatcgtt
    30781 aaactgcggg tcgacgttca catcctccgg gcggaattcc tccctcagcg ggtccgcgtg
    30841 ctcttccagc caggccatga ccagcgcgta aaccagcccc ggtgagtatt tgcggaacgg
    30901 gaagttatcc catgcaatca cgccggtgta cgtcatcacg cccatgcgga tctggtcgtt
    30961 acccagtgcc ttctgacttc ggataagttg cgcgtcttcc tgactgctca tgaagaactg
    31021 catggcgtca gacggcagat tttcggtaat gaaggcggtc aggctttcca gctggttcat
    31081 atgatgtgca ctcccgcgcg tctgtgcccc agcatgttac gcatgaccag cgaggcttca
    31141 gccagtaagg tggcgcgtgt gtcctcgctt tcctgccccg gataggtttc acggcgacca
    31201 atggtggcga actcgcccat aagatcggcc ttggcacggg cataaacggc ctttttgtac
    31261 tgtgcgacca gctgggtttc atcgcccagc ctggcccccg gtacgtctgc ggcgcgttca
    31321 tgcccctgcg ccttccagtg caccacaacc ggcgccagcg tggtgttcac ctcggccact
    31381 gcggcaagca gcgccatggc cgccgtgtcg tcgggtaaat cagccggaag cgtgcgtgac
    31441 ccctgaagct cagccagatc aaggtccggc cagaacggct cgccgtttgt caccgtctgc
    31501 ggtgtggatt tcagcggttt accgctgacg ctgaagctcg gttcgctcat gacgtgcctc
    31561 atatgaataa aaaggcgggc tgtccgggat tccacggcca tacagtttcc gtcactgatg
    31621 cctcctcccg cgcccgcccg gccctgcggg tagtcgttta cgctgcatta agggcgctga
    31681 tgcgtgccct gatgcgcgcc ctgatacttt ttacgccagc ggtacggctc agtgctgccg
    31741 cctgtgccag gtatgcgtct gccttttcca gtacggcagt atcattaatc gcggtggctc
    31801 tcggtttgcc cgcctcgtcg cgcagaagat acagcccggc aaatctgaac cacttcgcgc
    31861 ggatttcctc atgcagccgc cacgaggtgg tgacgttcct gaaggtgcgc gaaaaatacg
    31921 gttctatgct gtgtccggcc tcgctgttgt cggtggccca ctgcagcacc gtgtctgcca
    31981 cgaaggcggg cagggtgctg cggaagttgt ccggtgtggc ctggccttca ctgatggcaa
    32041 tgtctgccca gtcaagggcg cgactcaggt cgcccgtgtc gaacagccag atgatgcagt
    32101 acaccagtac cgggcaggcg tggcgctttc cgtcagtgat atagcgctcc gcgacgggca
    32161 gccagcgcgg caggagaatg tcgcgcttga acgccagccg ctcttcgcgg accggaagac
    32221 tgcgcagccg tgccacatcg cgatcaagct ccgcgcgtaa caggtggaag ctgaccgggg
    32281 aggccgtcag cgcctcccgg cgttcgagtg cctcctgtgt gcggattttc gcccggtgcc
    32341 gctggcaggg tgtcattgcg ccgccctccg tcctcagccg ccgacgcctg gtacggcttc
    32401 tgcgcccttg atggtcagtt tgtcgtaggc cgcgtaaatt tcgtcgtgct ccacggcgta
    32461 accttccatg cgcagatagt tgttttcaaa acgcttgcga tcgtcgttcc actccgcacg
    32521 gcgctgacgg gtgccgcgct gcgtgtagat gtgaaggttt gccagcgtgg tgacaatcag
    32581 gcgcccttcc ggcatgaacg gcggggtgta tgctgtcagc ccggcgatct ggcgaccgat
    32641 aagctgtgcg gccacttttt ccgtgggggc gtcgatggcg ttaagcagcg tcaccttgtc
    32701 ctgtgccaca aggtcgggcg caaccagtac caccagatcc ggatgaccgc gatatgactc
    32761 atcaatgagg gtatgcacga ggtctgccac ggctgcatcc agcccgataa agtcgccacc
    32821 cttgcccagc gtcgggccag cctccacgat ctgttttgcg ctgcggcctt tgacgatggc
    32881 atgccagccg gtgttgacgt cctcgccgtt tttgttggtt accgggtcgg tatcttcggc
    32941 cacgctggta ccgttaaagg cgatgcgcag gatatccagc gcaatctgtc tgttaacgaa
    33001 attctgaatg cggttaaaga actcgttttc acttcctgcg ttcgcccagg tgaccagtgt
    33061 ggtgtacagg aggtgggcgc cggaatcggt ttcagccagc ttgtattcat tcccggtaac
    33121 acccagcgga agggtgaagc gggcattttt tttacgtccg gtatggaggc ccggaacgcc
    33181 ggtggtaacc acctggcctg tcacctgttc aacatccagc acgttcggca gttttgataa
    33241 aaactcgtcg gagtgcatga tggcgtcacg cagcgcggtc tccttcgggt cagacagcgc
    33301 aaaatagcgt gtgacgtcgt caaccccgtt tgccgcgccc agcccggcag cgtacttacg
    33361 aagcagtgat tcggttttct ggttcagttg catgttgcgt gtccttaaac aaagtcaaac
    33421 ggtttttcgc tttctgccgg tgtgccagcc gggcgtggcg tgatgccggt ggctgacagc
    33481 ttcgtggtca ggtcgttaag tttttcggca atggctttga tttcttcttt gtcggtgtcg
    33541 tcccggcgac gggcggcaaa gcggcggtaa cgttcgcctt tttccggtgt gatggtgctg
    33601 aaggctttga tggcgtcttc cagcttcgct tttgcagcgg taaaggcttc ggccttaacc
    33661 tcgtcttccg gggcggcttt cacttcttct gccagctctg ccacctctgc ggcggcgtcg
    33721 gcgatttcgg cggcgatttc cgcgacttcg gcttttgctt cttccggcgt gtctgctgtg
    33781 ccctcgccag tggcagcggc gctgccgcct tccaccaggg tgatcagctt ttcaagcagt
    33841 gctttaattt cgtccatttt tgcgtcctcg cctgtatcag gcttactggt atcggtgttg
    33901 tcggtctgtc ctgcgctgaa caggcgtttc aggaatgatt caggtttacg gggggcaggg
    33961 gaggtgtccg gtgacaggct gcccagcgtg aacgtctggc agttcgccct ctgtgccgtg
    34021 tattcctcgt tgttcagggt gaagcggatt ttttccgtcc ccagactggc ggggatatcc
    34081 gtcaccgcca cgcccaggag atagtcgcgt ccgctccggg cgaagttctc cactatctcg
    34141 gcggatgtga acagcttctg ccccagtctg ttggcttcaa tcaggtactg gttagggacc
    34201 agccgtgcgt agaggcgggt tttaccgtcc tcgccggttt ccgctttcag tgagtccact
    34261 tcacccagat tactggtgta ctggcggtgt ccgatttctt cctcatgcca gggccacagc
    34321 agcgccgtgt aagtgttgcg ggaataggtt tcagcggcat cagtcagcca ctgcggatca
    34381 atttcgcggc cgtcaacggt cggtcctgat gtggcgatac atatccagtc ggtcatcaac
    34441 gttggctgcg gcatcgtggc ctcttttacg tgaaaatctg aagaactccg gtgagtgtcg
    34501 ggatttttac agcggggggc tactggctga attcggctgt cttcggataa cgcgggatag
    34561 ccgaacacag ccgaaatttt ctgcctgaat gcgggccgga tgcgccgcca taatgggcgc
    34621 atggctaaat attctgatga aatcaaagag gcggcgcgat cgctttacct gaagcggtgg
    34681 cacccccgcg atattgcggt ggaactcggt attccgccgc gcacggttta ccactggtgt
    34741 gatgtggggc ggtgggctgc cctgctgccc gctgaatccg ttgaggacgt gatcgcccgg
    34801 cgtattgatg tgctgaccgg acgggagggc aagacagagt gtgagcttgc cgaactgcgt
    34861 gaacttatcg cacagcacgt gaagatcatc gcccagaaaa acaaacacgc tgaaaggatg
    34921 gcggaaattg ccgccctgcg tccggcaggg catgaggacg gcgtggctgc ggggatctgc
    34981 ggcgggggag aagggaaaaa gcggcgctac cggaaaaacg acgtttccgg actgactgct
    35041 gaaatgctgg atgaatgggc gcgtaaaaat ctttatgcct accagctgca ctgccgcgag
    35101 aaccggcacc gtgagcgccg tttcatcctc aagagccgcc agatcggcat gacctggtat
    35161 tttgcatggg aggcgttcga agatgcggtg gtgaccggtg ataaccagat tttcttttct
    35221 gcgtcacggg tacaggcgga aatcttccgt gaatatatta ttgatttcgc ccagcagttc
    35281 gggatcacgc tcacgggtaa gcatatccgt ctgagcaatg gtgcaatgct gcgcttcctg
    35341 tccactaatg ccagtaccgc ccagggattt aacggccacc tttacggcga tgaggtgttc
    35401 tggatcccaa aattcacgcg actgcatgaa gtggcctcgg caatggcgac ccacgacaaa
    35461 taccgcacga cgtatttttc cacacccagc gccaaaaccc accaggctta tctggtctgg
    35521 aacggtgacg actggcgcgg ggatgacccg acgcgtcgtg ctgttgagtt tccgaaagaa
    35581 agcgcgatgc gtgtgggctg tgaatgccct gacgggatct ggcgctacat tatccggctg
    35641 gaagaggcgg tggcgggtgg cctgtctgca cgggttgata ttgaacgtat caggaacctc
    35701 tacaacccga ccacctatgc gatgctgtac ggctgcgagt ttgttgacag caaggatgcg
    35761 gtatttaagt tctccgaact ggtcaggtgc gaggtggaga tggagacgtg gggtgactac
    35821 gatcccacgg cggcacgtcc gttcggcaat cgtgaggtgt gggcgggctt tgacccgtca
    35881 aggtccgggg ataattccac gtttgtaatt gtggctccac ctgtccatga aggcgagcgg
    35941 ttccgtgtgc tcgccgtctg gcagtggcag ggatttaact tcacctggca ggccgatcag
    36001 atccgcgaac tgatgcagcg ctttaacatt acctatatcg gcatagatat caccggtatc
    36061 gggaaagggg tgtttgatat cgtcagtcgc tttgcgccac gtgaggctaa cgccattctt
    36121 tacagcgtgg agagcaaaaa ccgcctggtg atgaaaatga tagacgtggt ggcccataag
    36181 cgtatcgagt gggcgaaaga cgccattgat gaggccagcc gtgaacgcac ggagatcccc
    36241 gcgtcgttca tgtccatacg ccgcaccacc acaaaaagcg gtaacgcgct gacgttcgtg
    36301 gcggaacggt cggatgttac ggggcatgcg gatgtgtttt ttgccatttc gcacgccgtg
    36361 attaatgagc ctgtagatta tgactatgaa cgcccgtcca catgggcctt cgggaaagcc
    36421 gcatgagcaa gaaacgcaga aacaaaacgt cacgcgtgac gaaaaaagca ttcacgcccg
    36481 cacgtggcag cacaatcacc tttggccagc cggaaccggt gctgacaacc ggcacggact
    36541 accataacat ctggtatgac aacgaacaca accactggca gttgccgatt aaccgcctgg
    36601 cactggcgca gctcccgaac cttaacgccc agcacggtgg ggtgttgtat gcccgccgta
    36661 acatggtggc ggggggctat accggtggtg gcctcacaac ggaccagatg gaacaggcgg
    36721 tatttgacta cctgttgttc ggcgatctcg ccctgctcaa ggtgcgtaac gtattcggcg
    36781 acgtcgtggc gcttgagccg cttccgtcgc tgtatctccg gcgccgcaaa accggtgagt
    36841 tcgtggtgct tcaggaaggc cccgcgctgg tctatacgcc ggaagatatt atctttctgc
    36901 ggatgtatga tccgcgtcag caaatttacg ggctgccgga ttatatcggt ggcatccact
    36961 cggcgctgct gaacagcgag gccagccttt ttcgtcgccg ttactacaac aacggggcgc
    37021 atatgggttt tttgctgtat acgaacgacc ctgggatcac cacggagatg gaggatgaga
    37081 tccgcgagaa ggtggcggag tctaaggggc tgggaaattt ccgcaatctg tacatcaaca
    37141 tccccaaagg ttcgccggat ggggtgaaaa ttattccgat tggtgaggtg agcgccaagg
    37201 atgaattttc aaacatcaag gggatcagcg cccaggatgt gtttacggca caccgctttc
    37261 ctgccggtct tgcggggatt atccccacga acggggccac catccctaac ccggaaacga
    37321 cgcgtgaaac gtaccgcaag gatgaggtga taccgcttca gcgtaaaatc atgaacgccg
    37381 tgaacagcga tcccgatata ccggaacacc tttatctttg ttttgatgtg gatgccgggc
    37441 atgtggcgga agaaaaagcg gtgccggtcg ggtaaaatgt cctccccgga atacgtgtta
    37501 tcgggaggta agatgcggat ttttaaagcc cggtgtccgg actgtggcgc acctgctgtc
    37561 atccggaaga cagactggaa aacccctgaa cttgcggatc tgtactgtgc atgcagtgat
    37621 gtcgaatgcg ggcacacctt cgtctgtacg atgacctttt cccataccct cagccccggt
    37681 gcccgctccg gtcgcaatat ggtgaagttt cttgttgatg ccatgcgccc ggaagatcgc
    37741 cggtatgcgc ttgaactact gtcgcggcgt tcctgacgcg cttcgcttgt ctgctgcttc
    37801 gccggggcaa aaagaatttt taccccgact caccagctcc tttaacggca tgcagcgccg
    37861 tagataaaca gaattcgctt catgatttcg ctgcgcttgc aggcttcgat gactgatttt
    37921 tcctgctgcc gttcagcccg ttgcgccagt tcctgtcgtt ccttttccgt tggcttccgg
    37981 taatgcgtta tcttgtccgt gtacccgtcc acaaccagca ggccttcccg taccattttc
    38041 ccggcggcct gattaatgac ggttcttttc acgcccagtt tttctaccag ttctggggcg
    38101 ctgaaacgtg tgtgcgtttc caggtagcgt aaaatgattt cctttccggt cattcctggg
    38161 gtcttcattt ggcggctcct tcgttgttcc ggcgttcccg gagttcggtc agtgctgcaa
    38221 gtaaattctc ccaccacgtt tcttccacga ccgggttttg tcgtgaaagc agttgccggg
    38281 ccgactcaat cattttcgta agctccctgt cgctgatcct cctggtgtcg ttcatggtgc
    38341 gctcctttag ttggctgttt ctggttatgt tcatggtctt tgtttacctg cgtgattgtc
    38401 tgccagcggc tgaatgcaca tagcgggcaa ttgccgcttt gcggttaatt gtgaccagtg
    38461 ttctggtgag ttctgcgttg tgtttgtgtg cctgctcttc gtggtactgc cgcatgattt
    38521 ttccggcagg cgtgagcggc tccggctctc cgggcggacg gcgcggacga acggcgacaa
    38581 gccagctgcg gttggtgctg tccggtcgtc cgttgagtat gtcggtgatg atggccgtgg
    38641 tggtgcgatc gggactgacg accgtgattt ccagctcatc gcccggttgt ggccccgtgg
    38701 caatgttgtt cagggtcagt aagggcgtgc gctggcgcat catttcgtca aaacgctggc
    38761 gctgcatggc gcaataaaag cgggtcattg tggcgcctcc gtattcggtg cgggggtgat
    38821 atcaatgcgg cgggtgctgt cattccagta cgcggtgcgt ccgtcagcca ggcgcacgcg
    38881 ggcaccgtga cggacggaaa cgacggcgct ttcatgcagc tgcacgccca gcgcatcaag
    38941 ctcgcgggtg atttgtgcgg tgatgtgtgg ctcagcggtc agtgcgacac tcacgcgaac
    39001 gccgccagcg ggcgcactga tgccgccgtt tttcacggtt tccgtgtcag gtggcgggtt
    39061 tttcagctgc ctggtgagtt gttttcgctc tgtaggggtc agtgacctta accattcacc
    39121 caaatcacct tccggcaggg taagtctctg ctccctgcgt ccgtccggct gcctttctgg
    39181 cgggtcgttt atggtcagat tctggtcagg gggacagtta ttgccacgag tccaaggtgc
    39241 ggcaaagccg cactcaacgt caaaaccctc gtccgtgcgg tcttcgtttt ccggcttacg
    39301 tctgaccagt ttccaggtgt ccgggtgggt gcatatggtg tagcgctccc cgagtgacgg
    39361 ggcgcggacg ccgtaaatgc gttgcgggaa ttcgccgta
//
LOCUS       DAZGBC010000023        38046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGBC010000023 DAZGBC010000000
VERSION     DAZGBC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.023"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(120..407)
                     /gene="yebO"
                     /locus_tag="ACHK1J_001253"
     CDS             complement(120..407)
                     /gene="yebO"
                     /locus_tag="ACHK1J_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO7910556.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            complement(482..625)
                     /gene="mgrB"
                     /locus_tag="ACHK1J_001254"
     CDS             complement(482..625)
                     /gene="mgrB"
                     /locus_tag="ACHK1J_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO7910557.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            784..1023
                     /gene="yobH"
                     /locus_tag="ACHK1J_001255"
     CDS             784..1023
                     /gene="yobH"
                     /locus_tag="ACHK1J_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO7910558.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1167..1958)
                     /gene="kdgR"
                     /locus_tag="ACHK1J_001256"
     CDS             complement(1167..1958)
                     /gene="kdgR"
                     /locus_tag="ACHK1J_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO7910559.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2135..3508
                     /gene="yebQ"
                     /locus_tag="ACHK1J_001257"
     CDS             2135..3508
                     /gene="yebQ"
                     /locus_tag="ACHK1J_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7910560.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMADLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMYALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3558..4439)
                     /gene="htpX"
                     /locus_tag="ACHK1J_001258"
     CDS             complement(3558..4439)
                     /gene="htpX"
                     /locus_tag="ACHK1J_001258"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO7910561.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4631..6679)
                     /gene="prc"
                     /locus_tag="ACHK1J_001259"
     CDS             complement(4631..6679)
                     /gene="prc"
                     /locus_tag="ACHK1J_001259"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO7910562.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6699..7397)
                     /gene="proQ"
                     /locus_tag="ACHK1J_001260"
     CDS             complement(6699..7397)
                     /gene="proQ"
                     /locus_tag="ACHK1J_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO7910563.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7494..7991)
                     /gene="msrC"
                     /locus_tag="ACHK1J_001261"
     CDS             complement(7494..7991)
                     /gene="msrC"
                     /locus_tag="ACHK1J_001261"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO7910564.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8121..9404
                     /gene="yebS"
                     /locus_tag="ACHK1J_001262"
     CDS             8121..9404
                     /gene="yebS"
                     /locus_tag="ACHK1J_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO7910565.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9373..12006
                     /gene="yebT"
                     /locus_tag="ACHK1J_001263"
     CDS             9373..12006
                     /gene="yebT"
                     /locus_tag="ACHK1J_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO7910566.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12086..13525
                     /gene="rsmF"
                     /locus_tag="ACHK1J_001264"
     CDS             12086..13525
                     /gene="rsmF"
                     /locus_tag="ACHK1J_001264"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO7910567.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRPWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            13643..13879
                     /gene="yebV"
                     /locus_tag="ACHK1J_001265"
     CDS             13643..13879
                     /gene="yebV"
                     /locus_tag="ACHK1J_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO7910568.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13984..14175
                     /gene="yebW"
                     /locus_tag="ACHK1J_001266"
     CDS             13984..14175
                     /gene="yebW"
                     /locus_tag="ACHK1J_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO7910569.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14176..14832)
                     /gene="pphA"
                     /locus_tag="ACHK1J_001267"
     CDS             complement(14176..14832)
                     /gene="pphA"
                     /locus_tag="ACHK1J_001267"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7910570.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(15228..15569)
                     /gene="yebY"
                     /locus_tag="ACHK1J_001268"
     CDS             complement(15228..15569)
                     /gene="yebY"
                     /locus_tag="ACHK1J_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO7910571.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15582..16454)
                     /gene="copD"
                     /locus_tag="ACHK1J_001269"
     CDS             complement(15582..16454)
                     /gene="copD"
                     /locus_tag="ACHK1J_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO7910572.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16458..16832)
                     /gene="yobA"
                     /locus_tag="ACHK1J_001270"
     CDS             complement(16458..16832)
                     /gene="yobA"
                     /locus_tag="ACHK1J_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO7910573.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWRVVSVDGHKTKGHYTFSVK"
     gene            16971..17201
                     /gene="holE"
                     /locus_tag="ACHK1J_001271"
     CDS             16971..17201
                     /gene="holE"
                     /locus_tag="ACHK1J_001271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO7910574.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17303..17959
                     /gene="yobB"
                     /locus_tag="ACHK1J_001272"
     CDS             17303..17959
                     /gene="yobB"
                     /locus_tag="ACHK1J_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO7910575.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17983..18645
                     /gene="exoX"
                     /locus_tag="ACHK1J_001273"
     CDS             17983..18645
                     /gene="exoX"
                     /locus_tag="ACHK1J_001273"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO7910576.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18642..20702)
                     /gene="ptrB"
                     /locus_tag="ACHK1J_001274"
     CDS             complement(18642..20702)
                     /gene="ptrB"
                     /locus_tag="ACHK1J_001274"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO7910577.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20911..21570)
                     /gene="yebE"
                     /locus_tag="ACHK1J_001275"
     CDS             complement(20911..21570)
                     /gene="yebE"
                     /locus_tag="ACHK1J_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7910578.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21896..22252)
                     /gene="yebF"
                     /locus_tag="ACHK1J_001276"
     CDS             complement(21896..22252)
                     /gene="yebF"
                     /locus_tag="ACHK1J_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO7910579.1"
                     /translation="MKKRGAFLGLLLVSVCASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVVWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22319..22609)
                     /gene="yebG"
                     /locus_tag="ACHK1J_001277"
     CDS             complement(22319..22609)
                     /gene="yebG"
                     /locus_tag="ACHK1J_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO7910580.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22743..23921
                     /gene="purT"
                     /locus_tag="ACHK1J_001278"
     CDS             22743..23921
                     /gene="purT"
                     /locus_tag="ACHK1J_001278"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO7910581.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23977..24618)
                     /gene="kdgA"
                     /locus_tag="ACHK1J_001279"
                     /gene_synonym="eda"
     CDS             complement(23977..24618)
                     /gene="kdgA"
                     /locus_tag="ACHK1J_001279"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO7910582.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24655..26466)
                     /gene="edd"
                     /locus_tag="ACHK1J_001280"
     CDS             complement(24655..26466)
                     /gene="edd"
                     /locus_tag="ACHK1J_001280"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO7910583.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26701..28176)
                     /gene="zwf"
                     /locus_tag="ACHK1J_001281"
     CDS             complement(26701..28176)
                     /gene="zwf"
                     /locus_tag="ACHK1J_001281"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO7910584.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28514..29383
                     /gene="hexR"
                     /locus_tag="ACHK1J_001282"
                     /gene_synonym="yebK"
     CDS             28514..29383
                     /gene="hexR"
                     /locus_tag="ACHK1J_001282"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO7910585.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLVTGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29511..30953
                     /gene="pyk"
                     /locus_tag="ACHK1J_001283"
     CDS             29511..30953
                     /gene="pyk"
                     /locus_tag="ACHK1J_001283"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO7910586.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31084..32055)
                     /gene="lpxM"
                     /locus_tag="ACHK1J_001284"
                     /gene_synonym="msbB"
     CDS             complement(31084..32055)
                     /gene="lpxM"
                     /locus_tag="ACHK1J_001284"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7910587.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLMIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32175..33497)
                     /gene="mepM"
                     /locus_tag="ACHK1J_001285"
     CDS             complement(32175..33497)
                     /gene="mepM"
                     /locus_tag="ACHK1J_001285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO7910588.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33513..34445)
                     /gene="znuA"
                     /locus_tag="ACHK1J_001286"
     CDS             complement(33513..34445)
                     /gene="znuA"
                     /locus_tag="ACHK1J_001286"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO7910589.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34524..35279
                     /gene="znuC"
                     /locus_tag="ACHK1J_001287"
     CDS             34524..35279
                     /gene="znuC"
                     /locus_tag="ACHK1J_001287"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO7910590.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35276..36061
                     /gene="znuB"
                     /locus_tag="ACHK1J_001288"
     CDS             35276..36061
                     /gene="znuB"
                     /locus_tag="ACHK1J_001288"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO7910591.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(36275..37285)
                     /gene="ruvB"
                     /locus_tag="ACHK1J_001289"
     CDS             complement(36275..37285)
                     /gene="ruvB"
                     /locus_tag="ACHK1J_001289"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO7910592.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37294..37905)
                     /gene="ruvA"
                     /locus_tag="ACHK1J_001290"
     CDS             complement(37294..37905)
                     /gene="ruvA"
                     /locus_tag="ACHK1J_001290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO7910593.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9104 a         9826 c         9380 g         9736 t
ORIGIN      
        1 ataattattt gaagtcagac cagagtttta atttgaagca gttgcaccat gacagtgcgt
       61 ataatcagga tgaattgagt aacccttcaa cgacggggtt actcaagatt gaggaaggat
      121 tatcgttcgg cgaccaggcg aataatatct tcgtcttcaa ccgatttttg actctcaatg
      181 gtctttttat ctgctttctc tggttcgttt gcttcgcaca aacgacgtaa cagtgcattt
      241 tgacgttttt gctgatccag caacgcctca agcagttcaa tctgttcgtt agtccgagaa
      301 ctggcacgat taataaaaaa ccacaagatg agcccgataa gaagaaccac caccgataca
      361 accaaagacg caatgttcat cacgcctgaa tttacaactt cgttcatttc accacctctg
      421 agtagaggcg ctattctacc actgctggag aggaagaaaa tctagtgctg aaaaaatgat
      481 atcaccacgg gataaactgg ttaatggcac aaattccgct gaaaaattgt acatcctgat
      541 cgcacatcat gttgaatacc tgcgcccaaa gcagcaagca agccaacacc acgacaacca
      601 gaacgaccca tcgaaacttt ttcactccaa tctccgtttc acctacctta tgtcattaaa
      661 ctagcatgcg ttagttaaac agcgcctaac tatgtcgata ttcgtgcttt ttcggaatga
      721 gtcgcttgct tcagcgcata ttgccgctac gattaagcga acataaaaag agaagaggtt
      781 gtaatgcgat tcatcattcg cacagttatg ctgatagcac tggtatggat aggattatta
      841 ctcagcggat atggcgtttt gattggaagc aaagagaatg ccgcaggatt aggtttgcaa
      901 tgtacctatc tgactgccag aggcaccagc acggtacaat acttgcacac taaaagtggt
      961 tttttgggga taacagattg cccgctgtta cgcaaaagca atatcgtggt cgataatggt
     1021 taagtgtaat aaataaccag attctcactc atgagtaaaa tattcttata gcaactgcgt
     1081 ttaatctaac gccaccttca actttatttt tctcatgcta aataaagaac ctgtctgttt
     1141 gatattccga caggttctta ctctcatcag aacggatagt cgtgataacc catttgggca
     1201 gaaattttgc gcgctgcggt atgcaacatt gcgacatatt cctgtaaacg ctcttcagag
     1261 aaacgcaacg tcgggaagga gatgctcaaa ccggcaatga ccacgccaaa gcgatcaaat
     1321 accggaaccg caatgcatcg cagcccttct tcctgctctt cgttatcttc gccatacccc
     1381 tgctcgcgca cctggtccag aaccggtaat aacgcttctg tactggtgat ggtccgctcg
     1441 gtactgcgtt tatactccac gccctcaaga atttgcttca cttcatcgcg atcgcgccat
     1501 gccagcagta ccttaccaat cgcggtgctg tacagtggat tacgacgccc aatccgtgaa
     1561 tacatgcgca aattgtacat agagtcaatt ttgtgaatgt aaacaatact gtcttcgtcc
     1621 agtgcgccga ggtggatagt ttctttggtc aggcgggaga tctcacgcat ctggatatct
     1681 gcgctacgaa ttaaatcgac gttttgtaac gcgcgagcgc ccagttcaaa caatttcagg
     1741 gtcagggaat atttctccga ctccccttcc tgcgccacat aacctaaggt tttcatggtc
     1801 tgtaaaaagc gataaacggt gctttttgac atcatgacgc gctgcgacag ctcggttatc
     1861 cctatttcgc gctcttcacc cagcgcctgc aaaatgccaa aaacttttag cacggaagat
     1921 acagaatcag gctgtttatc cagatctgcg ttagccattt atcacctcat tgcgagtgtt
     1981 ttataaaaat cagaactgtt ttttattata atttcgcacc agggtggtcg caatccatct
     2041 tttgccggtt agttacaatt ctgcgacatc caccgtgaat atcagtgcta gaatcatacc
     2101 cctgttgatt attcaccaaa gatttaaaat tcctatgcca aaagttcagg ccgacggcct
     2161 gccattgccc cagcgatacg gtgcgatatt aaccattgtg attggtattt cgatggctga
     2221 ccttgacggc gcaatcgcca acgtcgccct gccaacaatc gccacggacc ttcatgccac
     2281 gccagccagt tccatctggg tagtgaacgc ctatcaaatc gccattgtca tctccctgct
     2341 ctcgttttcg tttctgggtg atatgtttgg ctatcgacgt atttataaat gcggtctggt
     2401 cgtttttctg ttgtcttcac tgttctgcgc cctttctgat tcgctgcaaa tactcaccct
     2461 tgcgcgtgtc atacaaggtt tcggcggtgc agcgttgatg agcgttaata ccgcccttat
     2521 ccgcctgatc tatccacaac gttttctggg tagagggatg ggcataaact cgtttattgt
     2581 tgccgtctct tctgctgccg ggccgacaat tgctgcagca atcctctcca tcgcatcctg
     2641 gaaatggtta tttttaatca acgtaccgtt aggtattatc gccctgcttc tggcgatgcg
     2701 ttttctgccg cccaatggtt ctcgcgccag taaaccccgt ttcgacctgc ccagcgccgt
     2761 gatgtacgcg ttaaccttcg gcctgcttat cactgcgttg agtggtttcg ctcaggggca
     2821 atcgctgacg ttaattgctg cggaactggt ggtaatggtt gttgttggta ttttctttat
     2881 tcgccgccag ctttctcttc ccgtaccgct gctaccggtg gatttactgc gtatcccgct
     2941 gttttcactt tctatttgca catctgtttg ctctttctgc gcacaaatgc tggcaatggt
     3001 ttccctgccc ttttacctgc aaaccgtcct cgggcgtagt gaagtcgaaa caggtttact
     3061 tctgacaccg tggccgttag caacgatggt gatggctccg ttggcaggct atttgattga
     3121 acgcgtacat gcaggattgc tgggtgcttt aggattattc atcatggctg cggggctttt
     3181 ttccctggtt ctgctgcccg cgtcacctgc ggatatcaat attatctggc cgatgatctt
     3241 atgtggcgct ggatttggct tgttccagtc acccaataac cacaccatta ttacctccgc
     3301 tcctcgcgaa cgtagcggtg gagccagtgg catgttagga acggctcgtc tactgggtca
     3361 gagtagcggc gcggcgctgg tggcgctgat gctaaatcag tttggagata atggtacaca
     3421 cgtctcgctg atggctgcgg ctattctggc agtgattgct gcctgtgtca gtggtttacg
     3481 tatcactcag ccacgatcca gggcataaaa aaaagcgcgt cgatcaggac gcgcttttta
     3541 gtggttactt cataatatta cttcaggtat tcacccgtac gcagagcttc aattcgttta
     3601 tccagcggcg ggtgagtcat gaacaactca ctgagcgatt tcgacttacc gttaatgcag
     3661 aaagccatca tgctggttgc ttcttgcggt tcatagctgg ttttcaggcg ctgcagcgcg
     3721 gcaatcattt tctcgcgacc aaccagtttt gccgaaccgg catcagcatg gaattcacga
     3781 tgacgcgaga accacatggt gataatgctc gccagaatac caaacaccag ttccagaacc
     3841 gttgcaaccg caaagtagat cagcgggttg ccgttgctct cttcaccttc atcacgattt
     3901 ccgcccataa aacccgcggc aagctgcgcc aggatacggg aaataaagat aacgaaggtg
     3961 ttcaccacgc cctgaatcag cgtcatggtg accatatcac cattggcgat gtggctgatt
     4021 tcgtgagcaa ttaccgcctc ggcttcatcc gggctcatgt tctgcagcaa accggtgctg
     4081 acagcaacca gagaggcatc acggcgcgca ccggttgcaa aagcgttgat gtccggcgca
     4141 tggtagatag ccacttgcgg catagcgatc cccgcctgac gagcctgggt tgctacagta
     4201 ttgaccagcc aacgttccct ttcgttacgc ggttgctcga tcacttcccc gccaacagat
     4261 cgtaatgcca tccatttgga catcagaagc gaaacgaagg aaccaccaaa accgaacagc
     4321 aaggccatga tcatcagccc ctgaacgctg ctcgactgta tccctgtcag gctcagtacc
     4381 agcccgaaaa cgaccattac ggccaggttc gttagcagga agagcgcgat tcgcatcata
     4441 attttctttt tacctcagtt taacaaaacg caatatgcga tacccacatc gtatgggtta
     4501 cgcgactatt ttcaagtctg gatagtgcgt aagtcaccag aaagacacaa ctttacattt
     4561 tgtagcatct gatttacggc atcttgccgc tgttaaaaaa tcaggcacaa tttcttgtgc
     4621 ctgattgata ttacttgacg ggtgcgggtt gttccgcggg tctggctttt tcaagcttcg
     4681 ccagatcgag tgcgatattc accgtctcat ccagataagg atccggctcc tggtaatctt
     4741 tcggtagatc atccagtttc ttcaactccg gtttaccttc gcgtttaaag cgttcgttca
     4801 aacgcgccag acgcgtcgca tcatcttcat tattctcttt ctcacgcaca gcgtaattca
     4861 gagaaacgat attgcgcttg tccttcatgg cgttgaagcg cgcgatatcc ttcatgatgt
     4921 tctggaactc aggatctttc gcgatacgcg cattatgttc cttcagcagc tccggttcaa
     4981 aggccgttaa atcgcctgat ttcacatagg tcgcggcatc aatgctatcc cacggcagcg
     5041 cgttatcttc gaatttctca cccgtttccg tttcttcatt acccgtcggc atgatgatgt
     5101 ctggcgttac gcctttacgt tgcgtactgc cgccgttaac gcgatagaat ttctggatcg
     5161 tgtactgcac agaacccagc gctggccatt caggacgtaa catctgatcg taaatacggt
     5221 tcaatgaacg gtattgctga acggtgcctt taccaaacgt cggttcaccc acaaccagcg
     5281 cacgaccgta atcctgcatt gccgcggcaa agatttctga agccgaagca ctgaagcggt
     5341 caaccagtac caccagcggg cctttataga aaacctgtcc gtcggtatcg ctgtcttcac
     5401 gaactttacc gttgttatca cggacctgaa caataggacc cgcaggaata aacagacctg
     5461 agagcgatac cgcttcggtc agcgccccac caccattgct acgcaggtcg atgatcacac
     5521 tgctgacatt ctgtttttcc agtttctgca gttgtacttt gacatcgtct gtcaaaccca
     5581 catagaagcc cggaatatcc agtacgccga ctttctcttt accgacggtc ttcaccgaca
     5641 ttttaaccgc gcggtcttcg agacgaatac gttcacgggt caatgttaca gtgcgggtct
     5701 tggtcccttt accagcaggt aaaatttcca gacgaacttt actgcccttc ggccctttaa
     5761 ttaaggcaac cacatcatca agacgccagc caatcacgtc aaccatcggc ttgcctgttt
     5821 gaccaacacc gacaattttg tcaccaacgc tgatagcttt actcttcgct gccggaccac
     5881 ctgccaccat cgaattgata acggtgtagt catcatccat ttgcagcact gcgccaatac
     5941 cttccagcga caaactcatt tcagtgttga actgttcggt attacgcggg gaaagatagt
     6001 tggtatgcgg gtcgatttca cgcgcaaacg ccgtcattgc cagcgagaaa acatcttcgc
     6061 tgttggtttg cgccagacga cgaatggcaa atttgtagcg tcgagtcagg gtttcacgaa
     6121 tttctttatc cgtttttcct gccagcttca ggcttaactc gtcgaatttg actttactgt
     6181 cccacagcgc gttcaactca gcctcgtttt tcggccaggg cgctttgctg cggtcaaggt
     6241 tataagtgtc gttgccggtg aaatccatcg gcttttccag taccgacaaa gcgtactggt
     6301 aacgttcaaa acgacgcttt tgcgccagat tgtagagatc gtagaaaacg tcgagtttgc
     6361 ctgaacgcag ttcatcgcct aactcggttt tctttttcgc gaactgttca acatcgcttg
     6421 ccagcagcac gttgtggctg taatcgagca gattcaggta gcggtcaaag attttggccg
     6481 aaaatgcctg atcgaggtcg aactggcgat aatgagaacg ggtgaagcgc gacgttacgc
     6541 gctcactcac cgtcgcatgc tgcgtctctt cctttaatac cggaatttga tcagcacgcg
     6601 tgatatcttc tacagcgaag gtctggcctg ctattgcaag caggccagct aacgcggtaa
     6661 gcctaaaaaa catgttcatg cctggcccgg cctccgtttc agaacaccag gtgttctgcg
     6721 cgcacaatca aagacatacc cgaattcagc tggacgcgga cgccgtcttt ggtgatttct
     6781 aatacggtgg catccatcgc gttttgaccc gctttcacct tcagggcttg tccgacagtc
     6841 agagctgaaa tgtcagaaac tggggtgtgc tgttcttcgc gaggtgcttt tgctgttttt
     6901 ggcgctttct ctaccggctt ttgcgcacga ggtttacgtt cagcgccttc tttgcggcgt
     6961 ggcgtggtcg gacgtggctt gcgttcgcga cgcggtgcgt cttctttctc accagcagct
     7021 gcggcagctt cgcgtttttt cgcttgctgt tcagcacgct gtgcctgaac acgcgctttc
     7081 gcttcttcaa gctgcttgcg agcatgctct acatgttgct cgtccagctc accgcatggg
     7141 ttgccgtcaa gatcgacacg cgttgcgccg ggtttaacac cgtaaaggta acgccagctc
     7201 gaagtgtaga gacgtaaagc ggatcgtaat tgcgttttgc tcaggttcat ttccccggca
     7261 acacgatcga ccaaatcctg aaaaataccg attttcagcg gacgcgcttc accttccgca
     7321 ctgaaacagt ggggaaaacg ttcggccaga aacgcgatta cttctttact gctattcaac
     7381 ttaggttgat tttccatgaa atttcctgat tacaacggac gtagccaaca agcgcaggca
     7441 tgaacaggcg tcattataat gacgctatca gtaaatgcta cgttatccgt tgattatcct
     7501 gcgacgctcg caaagaattt tttgtaatcc gtcgttgcaa gcaccttttc aagctgtgcc
     7561 acaagctgac gtaagccttg ctcgtcctcg tctgtaaagc gaccgaagac ggtactatcg
     7621 atgtcgagaa caccaataat ctgatttttc accaccagcg gcagaacaat ttcagaatta
     7681 ctcgccgcat cacaggcaat atgcccgtca aacgcatgaa catcctcgat acgctgcact
     7741 tgattgcggg caaccgcagt gccgcacacg ccacgcccga cgggtatccg gacacaggca
     7801 attttgccct gaaatggtcc gagcaccagt gtatcgtcct caagcaaata aaaacctgcc
     7861 cagtttacgt cagtgagacg ctcatataac aacgcactgg tgttcgcaag cgttgccaga
     7921 aaactggttt ctcccgccat cagcgcgtta aagtcgcgat ttaaatccgc gtaaaattct
     7981 gttttgttca ttatataatc acttggttgt cttacctgga actgccagcc tattaaaata
     8041 agcattaaat gcgttaatgc tcaagatcat tcccatcatg ggttaagatt aatgttaatt
     8101 cttattacat ttggcacgtc atggctctta acacaccaca aattacgccg acaaaaaaga
     8161 taacagtgag ggcaatcggc gaggaactgc cgcgtggtga ttaccaacgt tgcccgcaat
     8221 gtgacatgct gtttagcctg cccgagataa attctcatca aagtgcctat tgtccgcgct
     8281 gtcaggcaaa aattcgtgac gggcgcgact ggtcgctaac gcgcctggcg gcaatggcct
     8341 tcaccatgct gttgctgatg ccgtttgcct ggggcgaacc gctgttgcat atctggctgt
     8401 taggtattcg tatcgacgcc aatgtcatgc aaggcatctg gcaaatgacc aaacagggcg
     8461 atacgataac gggggcgatg gtctttttct gcgttattgg tgcccccctc attctggtgt
     8521 cctccatagc atatttatgg tttgggaacc gactgggaat gaatttacgt ccggtactgc
     8581 tgatgcttga gcgacttaaa gagtgggtaa tgctcgatat ctacctggtc ggcattggcg
     8641 ttgcttctat aaaggtacag gattatgccc atatccaggc gggtgtcggc ttgttctctt
     8701 ttgtggcgtt ggtgatttta acgacggtga cgttgtcaca tcttaatgtc gaggagctgt
     8761 gggagcgatt ttatccgcag cgccccgcta cgcgtaggga cgagaaactt cgtgtctgtc
     8821 ttgggtgcca ttttaccggc tatcctgatc agcgtggtcg ctgcccgcgt tgtcatattc
     8881 cgctacgcct gcgtcgccgt catagtttgc aaaaatgctg ggcggcgctg ttagcgtcaa
     8941 tcgttttgtt gttacctgcc aacctgttgc ctatttctat catttatctg aatggtggcc
     9001 ggcaggaaga tacgattctt tccgggatta tgtcgctggc aagtagcaac attgccgttg
     9061 cgggaatcgt gtttatcgcc agtattctgg taccgtttac taaagtgatc gtcatgttca
     9121 ctttactgtt gagcattcat tttaaatgcc agcaaggttt acgcacacgc attctgttac
     9181 tgcgaatggt gacctggatt ggtcgctggt cgatgctcga cctgtttgtc atatctttaa
     9241 ccatgtcgct gattaatcgc gatcagatcc tcgcttttac tatgggaccg gctgcgtttt
     9301 atttcggcgc agcggtaatt ttgactattc ttgctgtgga atggctggac agccgcttac
     9361 tttgggatgc acatgagtca ggaaacgccc gcttcgacga ctgaagcgca gattaaaaat
     9421 aaacgccgta tctcaccttt ctggctgctg ccgttcatcg cgctaatgat tgccggttgg
     9481 ctgatttggg acagttatca ggaccggggt aataccgtca ccatcgactt tatgtcggcg
     9541 gatggtattg ttccaggccg tacgcctgtt cgttatcagg gcgttgaagt cggaacagtg
     9601 caggatatca gcctcagcga cgatcttcgt aagattgaag tcaaggtcag catcaagtcc
     9661 gatatgaaag atgcactgcg cgaagagact cagttctggc tggtgacgcc aaaagcatcg
     9721 ttggcaggtg tctccgggct ggacgccctc gtcggtggga actatatcgg catgatgccg
     9781 ggtaaaggta aagagcagga tcactttgtc gcgctcgata cccaaccgaa atatcggctg
     9841 gacaatggcg atctgatgat ccacctgcaa gcccccgatc tcggttcgct gaacagcggt
     9901 tcattggtct atttccgcaa gatcccggtg ggaaaagtct acgactatgc catcaatccc
     9961 aataaacaag gcgtggtgat tgatgtcctg atcgagcggc gttttactga cctggtgaaa
    10021 aaaggtagcc gtttctggaa tgtttctggc gttgatgcca acgtcagtat cagtggcgcg
    10081 aaggtgaaac tggaaagtct ggcggcactg gttaacggtg cgattgcctt cgattcacct
    10141 gaagagtcga aacctgccga ggcggaagat acctttggtc tgtatgaaga tctggcccac
    10201 agccagcgtg gcgtaataat aaaactggaa ctgccgagtg gggccggatt aaccgccgac
    10261 tcgacgccat taatgtatca gggactggaa gtcggacagc taactaaact ggatttaaat
    10321 cctggtggta aagtcactgg ggaaatgacc gttgatccca gcgtcgttac cctgcttcgt
    10381 gaaaataccc gcatcgaatt acgtaatccg aaattatccc ttagcgatgc caatctcagc
    10441 gccctgctga ctggcaaaac cttcgagttg gtacccggcg atggcgagcc acgcaaagag
    10501 ttcgttgttg tgccaggcga aaaagcactg ctgcatgaac ctgatgttct gacgctgacc
    10561 ctgaccgcgc cggaaagtta cggtattgat gcgggtcagc cgctcattct tcacggcgtg
    10621 caggtaggcc aggttatcga tcgtaaactc accagcaaag gcgtcacctt taccgtcgcc
    10681 atcgagcctc agcatcgaga actggtaaaa ggcgatagca aatttgtcgt caacagccgt
    10741 gtcgacgtga aggtggggct ggatggcgtt gagtttctcg gtgccagcgc ctcagaatgg
    10801 attaacggcg ggatacgtat tctgccgggc gataaaggcg agatgaaagc cagctatcca
    10861 ctgtatgcca atctggaaaa agcgctggag aacagcctta gcgatttacc caccacaacc
    10921 gtgagtttga gtgcagagac gctgccggat gtgcaggcag gatcggtagt gctgtaccgt
    10981 aaatttgaag ttggtgaagt tattaccgtg cgtccgcgag ctaacgcgtt tgatatcgat
    11041 ctgcatatta agccggagta tcgcaacctt ctgaccagca atagcgtgtt ctgggcagaa
    11101 ggcggggcga aagttcagct gaatggtagt ggcctgaccg tacaggcatc cccgctctcc
    11161 agagcattaa agggagccat tagcttcgat aacctcagcg gtgccagcgc cagtcagcgt
    11221 aaaggcgata agcgtattct gtatgcttcc gaaacagcgg cccgtgcggt tggcgggcag
    11281 attacgcttc acgctttcga tgccggaaaa ctggcggtcg ggatgccaat tcgctatctc
    11341 ggtattgata tcgggcaaat ccagacactg gatctgatta ccgcgcgtaa tgaagtacag
    11401 gcaaaggcgg tgctctatcc ggaatatgtc cagacttttg ctcgcggagg tacgcgcttc
    11461 tcagtggtca caccgcaaat ttcggcagct ggcgttgagc atctagatac tatcctccag
    11521 ccgtatatca acgtcgaacc aggccgtggc aatcctcgtc gcgactttga attacaagag
    11581 gccaccatta ctgattcgcg ttacctggat ggtttaagca ttattgttga agcgccggaa
    11641 gccggttcgt taggcatcgg tacgcctgtg ctgttccgtg gtctggaagt aggtacggtt
    11701 accggaatga cgctggggac attgtcagat cgcgtgatga ttgcgatgcg catcagtaaa
    11761 cgctatcaac acctggtgcg taacaattcc gtcttctggt tggcatcggg ttacagtctg
    11821 gattttggtc tgacgggcgg cgtagtgaaa accggcacct ttaaccagtt tatccgtggc
    11881 ggcatcgcct tcgccacgcc tccgggtacg ccactggcac cgaaagccca ggaaggcaag
    11941 cacttcctgt tgcaggaaag tgaaccgaaa gagtggcgtg aatggggaac tgcgcttccc
    12001 aaataatgcc cactgctccg gcgtgcctgc gccggagcgt ttatgctaaa ctgcgcgcct
    12061 gtttttttgc cagtggtata tgctcgtggc ccaacacacc gtttatttcc cggacgcctt
    12121 tctgacgcaa atgcgcgaag cgatgccttc gacgctctct tttgatgatt ttcttgccgc
    12181 ctgtcagcgc ccgttgcgcc gcagcattcg cgttaatacg ctgaaaatct ccgttgctga
    12241 tttcctgcaa ttaaccgctc cttatggctg gacgcttacg ccaattccgt ggtgtgaaga
    12301 aggtttctgg attgaacgcg acaatgaaga tgcattgcca ttgggtagta ccgccgagca
    12361 tttaagcggc ctgttttata ttcaggaagc cagttcaatg ttgcccgttg ccgccttgtt
    12421 tgctgacgat aatgcaccac agcgggtgat ggatgtcgca gctgcgccag gctccaaaac
    12481 gacgcaaatt gccgcgcgga tgaataacga aggggcaatc cttgccaatg agttttccgc
    12541 cagtcgggta aaagtgttac atgccaatat cagccgctgt ggtatcagta atgttgcgct
    12601 cacacatttt gatggccgcg tgtttggtgc ggcattgcca gaaatgttcg atgccatttt
    12661 gctggacgct ccctgctccg gcgaaggcgt ggtgcgtaaa gatcccgatg cgctaaaaaa
    12721 ctggtcacca gaaagcaatc aggaaatcgc agctacccaa cgggaactga tcgacagtgc
    12781 ctttcatgca ttacgccctg gcggtacact ggtttactcg acctgtacct taaaccggga
    12841 agaaaacgaa tccgtttgcc tgtggcttaa agagacttac cccgacgcag tagagttttt
    12901 accacttggc gatctcttcc ctggtgcaaa caaagcgctg accgaagaag gctttttgca
    12961 tgttttccca caaatttacg actgcgaagg cttcttcgtt gctcgtctgc gtaaaactca
    13021 ggccattccc gccttaccca ccccaaaata caaagtgggc aatttcccgt tcagcccggt
    13081 gaaagatcgc gaagccgcac aaattcgtca ggcggctgca agtgttggct taaactggga
    13141 tggaaacctg cgcccctggc agcgtgacaa agaactgtgg ttgttcccgg tgggcattga
    13201 agccctgatc ggtaaagtcc gattttctcg cttggggatt aaacttgccg agacgcataa
    13261 caaaggttat cgctggcagc atgaagcggt tattgccctt gcctcccccg acaacgagaa
    13321 cgcttttgaa ctgacaccgc aagaagcgga ggagtggtat cgcgggcgcg atgtttaccc
    13381 gcaagccgcg ccagtggcgg atgacgtgtt ggttactttc cagcatcagc cgattggttt
    13441 agccaaacgg attggttcgc gactgaaaaa cagctacccg cgtgaactgg tgcgggacgg
    13501 gaaacttttt accagtaacg catgacagcg cacaaaaaaa gcgcactttt tgactggcac
    13561 attcggcggc ctcaactagg ctgaaaaatg gtgcgatcgg actggtcgta ccacaatcgg
    13621 cagctaaatg gagagcacga agatgaaaac gagtgtgcgc ataggcgctt ttgaaatcga
    13681 cgacggcgaa ttacacggcg aatcgccggg tgatcgaacg ttaaccattc cttgtaaatc
    13741 tgaccccgat ttatgtatgc aactggatgc ctgggatgct gaaaccagta tccctgccct
    13801 gctaaatggc gaacactctg tcctttaccg tacccgttac gatcaacaat ctgatgcctg
    13861 gattatgcgt cttgcctgat ccaaaaagaa cccgtcggca tggcgggtta tttgtcctgg
    13921 ttattccccc gttgtaaaat ctctcctaaa cttaacggta cggcaccaca cttcggggat
    13981 gaaatgttcg cgctggtact ttttgtttgc tacctggatg gcggttgtga agatattgtt
    14041 gtggatgtct acaacacgga acagcagtgt ctttattcta tgagcgatca acggatccgc
    14101 catggcggtt gttttccgat tgaggatttt atagatggtt tctggcgacc agcacaggag
    14161 tatggtgatt tttaattatt gcaattgcac gaaagtcagt tcgcccccaa agacagcgcc
    14221 ggtatcaata taatgcaggt tgccaatatc cacgcgatgg cgcaacggtg tatgaccaaa
    14281 ccagaaatga tcagcacctg taattccctg ccctttttgg cgttcactta atcgcgagcg
    14341 gctccacaag acctgatgca aatcaacgtc cttttgccat tcataaacat catctggata
    14401 atcggcatga gcaataacat gtttgccggt gcggctgtgt acttcaagaa taaagggcaa
    14461 atgctgacat ttttccagcg ccgttttcgc ttgtttctgt tgattatctg ccagcgcaat
    14521 aaaccagtcg ccgccattca tcaaccacaa agacatctgc tgggatgcca gcgcatccat
    14581 cgccatctgt tcatgattgc ctcttaccgc acgaacccaa tgttgttcca gtaactgcag
    14641 acaacgtaaa ctttgcggcc cacgatcgat aacgtctccc actgagataa gtaaatctcg
    14701 ccacggatca aaacgacaat gccataattt gcggcgcaac tgctcaagac aaccgtgtat
    14761 atcgccagaa agccagatat gtcgccattg atgacccgca attctctgat aaacgggcgc
    14821 aggctgtttc atcaatattt tcctcccgcg ctaaagatca cataatctta acaagaatgt
    14881 taaaaaacgc tggactcaga cagtagagtg tgtgttatgg ttgactataa agtcagcgaa
    14941 ggaaatgctt ctggctttta acagataaaa agagaccgaa cacgattcct gtattcggtc
    15001 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
    15061 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
    15121 tcaataaaaa gcgcggtggt catcagctta aatgttaaaa accgcccgtt ctggtgaaag
    15181 aactgaggcg gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
    15241 tcagcccgtt ctataaacgg tgccagactc attttttcgc cgggattgtt gggatcatca
    15301 atctgaatca ccgaaatggg ttgggcttta gtcttcccac tggcaacttc cttctgtgcg
    15361 atatcgttta aaggatactg cacgagggta cttgggttaa tgacatacaa agcattaccc
    15421 ggtctgcaag tcagcatcac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
    15481 aaacggctga cggtaatcac ctgcggtgca gccagcgccg ctgcagaact ggtgagtaac
    15541 agaaacgcca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
    15601 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
    15661 cacacaaacc agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa
    15721 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
    15781 tggccccagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
    15841 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
    15901 tgaatagcct ggtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
    15961 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
    16021 ttcgccgtta ccccttcatt cagcgtcgca tgccccactc ccgccagcag aataaattgc
    16081 gcggtagtga gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc
    16141 aacgttacga gggcgagaac aatttgccat aaccagaccc caccaaactg cgtttgcaaa
    16201 acagcctgcc aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc
    16261 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
    16321 cgcagaaaac gcttcgtcag caagcgacga atcgttaagg gggccagcca ggcaccgtac
    16381 attgcgaagc caaaaaccag catcagcgag gtgaaatgga taaatcgcag cgcgatccag
    16441 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
    16501 tccaccgaaa caacacgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt
    16561 ggaacaatca gttgcttttg gtcctgctca tttcgcttcg ccggtaatgt tttaatattt
    16621 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacacc ttccgagaag
    16681 tttaaagtga ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc
    16741 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc
    16801 gcgtagcgaa gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt
    16861 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
    16921 ccaggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
    16981 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
    17041 gggtggcatt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
    17101 agcctgaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
    17161 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
    17221 tttcttacat aacgcctgct aaattatgag tattttctaa accgcaccca taatttgcag
    17281 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
    17341 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
    17401 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
    17461 gcgacgtgcc cttcctgccc cacccgatct ctcactgtta gacccgcttt gttatgccgc
    17521 aacgacctgg cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
    17581 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggatctacc atcagagtca
    17641 tggtgcatgt ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
    17701 tatggatatg gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga
    17761 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
    17821 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
    17881 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
    17941 tatcattcca ttacgctagg ctttttcggc ctggagcatg ccatgttgcg cattatcgat
    18001 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
    18061 ggaaaaatcg tcaaccccat gagccacctg gtgcgccccg atcgtcctat tagtccacaa
    18121 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
    18181 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc ataacgccag ctttgaccgc
    18241 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
    18301 tggccaggga tcaagtacag caatatggcg ttatataaaa cacggaagct caatgtacag
    18361 acgccgccgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
    18421 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
    18481 cgtccgtcgt tgatgacgac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
    18541 gttgccgaac gcgatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
    18601 ccggagctgc gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagcg
    18661 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
    18721 gatttaaagc gaccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
    18781 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat
    18841 tgcacctgag aatcgtgcaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
    18901 acgttgtcat atgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattc
    18961 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
    19021 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
    19081 gcaacgccca tcaacatgcc ccccgcactc ccgcccatcg cataacaaag cgaaggagag
    19141 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
    19201 ttcttcagaa atttaccgtc ttcgtaccat tgttgcccca gctcaccgcc gccgcgaaca
    19261 tggacaatgg cgtagacaaa accacgatct aacaaactca agcggctaaa actgaaatcg
    19321 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgccctttg
    19381 cgaaaatgtt tgcgatggta gaccaacgaa accggaactt cgacgccatc acgggcgact
    19441 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac caggaacttc cgtttgtttt
    19501 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
    19561 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
    19621 acataggccg gatcatcaaa ggcaatacca atgacttccc gggtcttgcg gttaatttgg
    19681 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
    19741 aacccttcca gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca
    19801 cgcatacggg tacggtataa gccaaagttt ttgccgttgc ggttggaacg cagataaaaa
    19861 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
    19921 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
    19981 aaatgaatga ctacatagtg cttcgaggtc gttttatgca ggctgacgta ataggtatcg
    20041 tctttttctt cgtaaatcag tttatcttgc gatgctggcg taccgatggc gtgacgccag
    20101 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
    20161 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt
    20221 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
    20281 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagcc
    20341 gcgcgcttgt tggcatcgag caatatttcc cactcgtccc actcttcact gaacgccgat
    20401 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
    20461 tttttgatgt agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag
    20521 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
    20581 tgcaggtagt ccaggacttc cggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
    20641 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
    20701 atgttattgt tctttctggt tgaaacatct tataagggtg gcaaaactca ccggggatgc
    20761 aagcgaaaca gggcagtcat tgcttagatg atgacaggta atggcgcgaa tatcgaatgt
    20821 tatgcagaca gagaaaatca gcctgttcaa atggctgtgc ggttctggat agcccgaaat
    20881 agtcaacttc aggctatcca gagagcggga ttattccgcc aaagtgcgtt tttgctgttc
    20941 gagatcgcgt tcaatgcctt cacgaacatc ctgggggatt ttcagcgcgt cacccagtgc
    21001 attcaggtaa ctgcgttcca taaaatggtc aatatcaata gccgcgcagc tcaggaaata
    21061 gatttccagc gcctcttctt cattgcggac cccggtagcc aggcgttgtg gatccagcgg
    21121 ttgttcgatt gcctgctcaa tgagtacccg cccctgctct tccacgccgg cttcacgcaa
    21181 ttgctggtcg attgccgcac gttctttggc atcaatatga ccatcacttt tagcggcaaa
    21241 gaccagcgca aggatcaaac gttccgtacg ctcatcgagc ggcgtacttt gcgcgccaaa
    21301 ctgcggttcg tcctgatgcg ccgcgcgaat tttatctttg tatttattcc acagcaccgt
    21361 acccgctacc gctccgccgc caaccagtaa cgcgttggtg ccatatttcg taagaagttt
    21421 acgtgctgat ttatttgcga ccagcagccc agccagtccg cctaatgcgc ctggcactaa
    21481 cagtttgccc aatccctgat ccgcagacga ggaggtagaa gaactgcttt gcccaagcag
    21541 ggattgcagt tgatttaacc agttagccat atttgctcct caataaccat ttttagtgcc
    21601 tgtagcatag cggatgagga tgtcagaaaa tgtatatcga gacaaaagat gcgcaaataa
    21661 gcggggcggt tggccgttat tcaaagaaat tcgcgccagg taaggtgttt actatatatc
    21721 aggtgataag ggtattttag gtgaaagtag tttgacgttt tgagtggcac tgtatgatgc
    21781 tgattgcccg atgctacgct gttgcgtctt ctcaggccta cgattgtggt gtgttgtggg
    21841 cggacaaggc actcgcgccg catccgccaa cggtcaatcg aattatcaga agccattaac
    21901 gccgctgata ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg
    21961 cacttttacc acgtacggtt agcggtactg accatttatc atctttaccc tgaatgtcct
    22021 gcaaactgac ccataccacg ggatcggcct gaccgacaat tttttgatca tctgcccagc
    22081 gcgccacgcg attttgttga taatcacgtt ttacgctcgc ggcaattccg gcagcatcca
    22141 gaccttcaca ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg
    22201 atgcgcagac agaaaccaac aacagcccta aaaacgcccc tctttttttc atgtttttct
    22261 ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc
    22321 aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgacctgt ggggacggta
    22381 atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa
    22441 gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagccag gtgtcaagca
    22501 gatccgccgt atcaagcatt ttgtcataag catcggcttc ctttttgctg gtaaacgaca
    22561 ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct
    22621 cataataact gtgattttat acagtatatt tcttttcggt tgagaaatca acaccagcaa
    22681 taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg
    22741 ttatgacgtt attaggcact gcgctgcgtc cagcagcaac tcgcgtgatg ttattaggct
    22801 ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg
    22861 ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta
    22921 atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg
    22981 tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga
    23041 atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc
    23101 tggcggcaga agagctgcag ctgcccactt ccacttatcg ttttgccgat agcgaaagcc
    23161 ttttccgaga ggcggttgcc gccattggct atccctgcat tgtaaaaccg gtgatgagct
    23221 cttccggcaa ggggcagacg tttattcgct ctgcagagca acttgctcag gcatgggaat
    23281 acgctcagca aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt
    23341 ttgacttcga aattaccctg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac
    23401 cagtaggtca tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga
    23461 gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg
    23521 gttatggtct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg
    23581 tctcccctcg tccacatgat accgggatgg tgacgttaat ttctcaagat ctctcagagt
    23641 ttgccctgca tgtacgtgcc ttcctcggac ttccggttgg cgggatccgt cagtatggtc
    23701 ctgcagcttc tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg
    23761 tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg
    23821 gcagccgtcg tctgggggtg gcactggcta ctgcagagag tgttgttgac gccattgaac
    23881 gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaaacgcta
    23941 caaaaatgcc cgatcctcga tcgggcattt tgacttttac agcttagcgc cttctacagc
    24001 ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct tccagcgcat ctgccggaac
    24061 cagccaggaa ccaccgatgc acagcacgct tttcagcgcc aggtagtcac ggtagttagc
    24121 cggagaaata ccacccgtcg ggcagaaacg gacctgggag aacggacccg cgatcgcctg
    24181 cagggctttc acgccgccgt tagcttcagc cgggaaaaat ttgaactctt tcaaaccgta
    24241 gtccataccc agcatcagtt cggaaacagt gctgatcccc ggaatcagag gaatagtccc
    24301 ttcggtagca gctttcagca gcggctcggt cagacccggg ctaattgcga actgtgcacc
    24361 cgcttcggtg acttctgcca gctgctgtgg attcagcacc gtaccggcac ccacaatcgc
    24421 ttcaggcact tctttggcga tagcacggat agcgtcaact gcacactcgg tacgcagagt
    24481 cacttccaga acgcgcaccc caccagcaac caacgctttt gccatcggca ccgcgtgttc
    24541 cagttttttt accacgataa ccggtacaac cgggccggtg gtcaggattg attctgcact
    24601 tgttttccag tttttcatca gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa
    24661 agtgatacag gttgcgccct gttcggcacc ggacagtttt tcacgcaagg cgctgaataa
    24721 ttcacgtcct gtccccacgc gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc
    24781 cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc tgtccattca cacgaatgat
    24841 gtccccgtcg cgcacttttg ccagcagccc gccatcgtag gcttctggtg ttacgtggat
    24901 agctgacggc actttacctg aagcgccgga gagtcgtcca tcggtaacta acgcaatttt
    24961 gaaacaccgg tccaataata caccaagtgg cggcatgagt ttatgtaatt ctggcattcc
    25021 gttcgctttt ggcccctgat gacggacaac aacgacacaa tcgcggtcca gcaaacccgc
    25081 ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca accgctggcg cttcaatcac
    25141 ctggttctca accggcacgg cagaggtttt cataaccgca cggcccaggt taccgcttaa
    25201 cacttttgtc ccaccatgat gagagaaagg ttgttcgaag gaagcgatca cattgctgtc
    25261 gagtgatttt tccgcccctt cccgccagtc cagttcacca ttattcagcc atggttcaag
    25321 ggtataacga gacagaccaa agcctgccac cgtattgaca tcttcatgca gcaggcctgc
    25381 tttgagcagt tcacgcacca gaaccggtac gccacctgcc gcctggaagt ggttaatatc
    25441 ggccggaccg ttcgggtaga gacgagccat cagcggtaca acatcagaaa ggtcagagaa
    25501 gtcatcccag ttaatctgaa taccggccgc gcgcgccatc gccaccaggt gcatggtgtg
    25561 gttagtggaa ccaccggtcg ccagcagtgc aacgataccg ttcaccacca ctttctcatc
    25621 gatcatctta ccgatcggca tccattcatt accattaccg gtcatgcgtg taacctgacg
    25681 cgcagcggca gcggtcaaag catcgcgcag cggagaatcc ggatgaacaa aagaagagcc
    25741 tggcaactgc atccccataa actccaccac catctggttg gtgttggcag taccgtagaa
    25801 agtacatgtt cccggcgcat ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc
    25861 cactttacct tcggcataaa gctggcgaat acgcactttt tctttatttg gcaaaccgct
    25921 tgccatcggt ccagacggca caaacaccgc aggcaaatga ccaaacgaca gggctgccat
    25981 cgtcagaccc gggacaatct tgtcgcacac cccgaggaac agagcaccat caaacatgtt
    26041 atgggacagc ccgaccgccg cagacatcgc tatcacttcg cggcttagca gcgacaactc
    26101 cattccatcc tgcccctggg tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg
    26161 accaaccgca ttcgcttcat gcagggcttt acgaatgatt tctggatagt gttcataagg
    26221 ctggtgagcg gagagcatgt cgttatagga ggtgatgatg gcgatattgt tacgcaacat
    26281 gcttttcaaa gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc
    26341 gcatgccaac tgcgaacgat gaacggtcga cgttttcgct tgttctatcc gggcgagata
    26401 agcagagcga gtctcgcgcg aacgttcaat gattcgattt gttacgcgta acaattgtgg
    26461 attcataaag gctcctgaaa atgagttgtc agagcaggat gattcacaat gcgtttcatt
    26521 cagaggattt atgagtgaaa cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc
    26581 agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt
    26641 tctacaatct gtgcaagatc atgttaccgg taaaataacc ataaaggata agcgcagata
    26701 ttactcaaac tcattccagg aacgaccatc acgggtaatc atcgccaccg aggcaacggg
    26761 tccccaggtt ccggcctgat acggtttcgg cgcatcattg tccatcgccc acgcctcagt
    26821 aatggagtct acccatttcc aggcttcttc cacttcgtcg cgacgtacaa acagtgcctg
    26881 aataccacgc atggtttcca gcagcaaacg ttcataggca tccgccagat gcgtctgatt
    26941 aaaggtttct gaatagctca gatccagctt ggtgatttgc aggttatgtt tgtggtcaag
    27001 gccaggaact ttattcagta cctggatatc cacgccttca tcaggttgca gacggatagt
    27061 cagtttattc tgcggcagat cctgccacga ctctttaaac agattcagtt caggtgtttt
    27121 gaaatagacc acgacttcag aacatttggt cggcagacgt ttaccagtac gcaggtagaa
    27181 tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg atcgccacga aggtttctgt
    27241 attgctgctc ttgttcgcgc cctcttcttc cagatatccc ggcacttttt tgccctgggc
    27301 gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt acgttggagc ggtcgatgcg
    27361 gcgcagagac ttcagtactt tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga
    27421 cggcggagac atcgcaatca tgcaaagaat ttgcagcagg tggttctgga tcatgtcgcg
    27481 catctgaccg gctttatcaa aatagcccca gcgcccttcg atccccactt cttctgccac
    27541 ggtaatctca acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca gggagttagc
    27601 aaaacgcagc gccaacaggt tcagcaccgt ttctttacca agatagtggt cgatacggta
    27661 aacctggcac tcctcgaagt attcgccaac ctgatcattg atttcctgcg aggtcgccag
    27721 cgacgtcccc agcggtttct ccatgactac gcgtgccggt ttagcattca gttttgcctc
    27781 gccaagccct ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt agttaatggt
    27841 gatacgattt ttttgatcca gcatcgcacc gagacggttg aatgcagcag tgtcattgac
    27901 atcgagatta caaaaatcca gacgtgcact cagggtgtcc cataaacctt catcaatggt
    27961 ttctttcatg aaagtttcga gcgcctcgcg gacaactttg gtatatgccg ctttatccca
    28021 gtcagcacgc cctacgccga taatccgggt gtccgggttg agctgaccgg ctttttccag
    28081 ttgatacagg gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac
    28141 caggtcacag gcctgggctg tttgcgttac cgccatgtca ttctccttaa gttaactaac
    28201 ccggtactta agccagggta tacttgtaat tttcttacgg tgcactgtac tgcttttacg
    28261 agcttgcgaa aactgtatac gcttatccac ccgtgcgatt acggtaaaag cgcgcaaagt
    28321 gcggcaaaac tgataaaaaa atcatcgttt tccattgggt aaaaatctga cactgatcat
    28381 gttatgaaaa aaaataacaa cttttttatc tgcttttgtc attaacacag cacacaggcg
    28441 taatatctga caaaactgca tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga
    28501 gtctctttac atcatgaata tgctggaaaa aatccagtct cagctggaac atttgagcaa
    28561 atcagagcgc aaagttgccg aggtcattct ggcttcgccc gataacgcga tccattcgag
    28621 tattgctgct ctggcactgg aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg
    28681 cagcatggac acgcgcggtt ttcctgattt taaacttcat ctggcacaaa gtctggcgaa
    28741 tggcactccc tatgttaatc gcaacgtcaa tgaagatgac agcgttgaat catacacggg
    28801 gaaaatattt gagtccgcaa tggcaacgct cgatcatgtc cgtcattcac tggataaatc
    28861 tgccatcaac cgcgccgtcg acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt
    28921 aggctcttca gccgccgttg cccacgatgc gatgaataag ttctttcgtt ttaatgttcc
    28981 ggtggtgtac tccgatgata tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg
    29041 agacgtggtg gtgctgattt ctcacactgg aagaacaaaa aatctggtcg agctggcgca
    29101 gctggcacgc gaaaacgacg ctatggtgat tgccctcacc tctgcgggta ccccgctcgc
    29161 ccgggaagca acgctggcaa ttaccctcga cgtaccggaa gatactgaca tttatatgcc
    29221 catggtttct cgacttgcac agctgaccgt gatagatgtg ctggtgacag gatttacttt
    29281 gcgacgcggt gcaaaattca gagataactt gaagcgggtc aaggaagcgc tgaaggaatc
    29341 gcgttttgat aagcagttac ttaatttaag tgacgatcgc taaaagcgac tgtcactgtc
    29401 ctaatcttat acgacatccg aatgagatta atttatcgcc atcgcggcgt tatttcattc
    29461 ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac atgtccagaa
    29521 ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc gataataacc
    29581 ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct cacggctcgc
    29641 ctgaagatca caaaatgcgc gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc
    29701 atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc tttaaagaag
    29761 gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg ggtaaaggtg
    29821 aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac gttgtgcctg
    29881 gcgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa gttcagggca
    29941 tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa ggtatcaaca
    30001 aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca gacattaaga
    30061 ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt ggcgaagatc
    30121 tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg
    30181 ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga tgacatcatc ctcgcctctg
    30241 acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg
    30301 gcattcagaa agcgttgatc cgtcgtgcac gtcagcttaa ccgcgcggta atcacggcga
    30361 cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa gtcatggacg
    30421 tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa actgccgctg
    30481 ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa
    30541 tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat gtggaagaag
    30601 ctattgccat gtcagcaatg tacgcagcta accacctgaa aggcgttacg gcgatcatta
    30661 ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct ggtctaccaa
    30721 ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat cgtggcgtta
    30781 cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc
    30841 tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc cagggcgacg
    30901 tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag taagtacgtt
    30961 gccggatgcg gcgaaaacgc cacatccggc ctacagttca atgatagttc aacagatttc
    31021 gaatattctg aagcaaactt gaacttatca tcaggcgaag gcctctcctc gcgagaggct
    31081 tttttatttg atgggataaa gatctttgcg cttatacggc tggatttcgc ccggtttgcg
    31141 agttttcagc aattttagta tccaggtgta ttgttctggt cgcggaccaa caaaaatctc
    31201 gacttcttca ttcatccgcc gcgcaatcgt atgatcatcc gcctctaaca gatcatccat
    31261 cggtgggcgc acctgaatca tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg
    31321 tacaacgcgc gcacggcaca ctttcatcaa acgaccaatc gcgggcaacg tcgctttata
    31381 ggtggcaaag aaatccacaa attcgctgtg ctctgggcca tgatcctgat cgggtaaata
    31441 atatccccag tacccctgac gtaccgactg gatgaatggt ttaataccgt catttctcgc
    31501 atgcagacga ccgccaaagc gacgacgcac cgtgttccag acataatcaa aaaccgggtt
    31561 gccctgatta tggaacatcg ctgccatttt ctgcccttgc gaggccatca gcatggcagg
    31621 aatatcgacg gcccaaccgt gcggcaccag aaagataact ttctcgttat tacgccgcat
    31681 ctcttcgatg atctccagcc cttgccagtc aacgcgcggc tgaattttct ccggcccgcg
    31741 tattgccaac tcagccatca ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac
    31801 aatcgcttcg cgttcagctt cactacgttc tggaaagcag agcgacagat tgattaacgc
    31861 acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg
    31921 atcacggaac tttggcggcg ttaaagcgat acccgccatc gctgctacgc ccagccatgc
    31981 tccccagtag cgcgggtggc gaaaggattt atcaaactca ggaatgtatt cgctattatt
    32041 ttttttcgtt tccatgcttt tccagtttcg gataaggcaa aaatcaatct ggtgatagtg
    32101 tagcggcgca acttgccccg caccaaataa aaaaagccgg tactgactac gtaccggctg
    32161 cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg
    32221 aattcgcgac gatcggagcc ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg
    32281 tttacggcct gctggtttat ccatacttca tagtgcagat gcggcccggt tgaacgtccg
    32341 gtattaccgg aaagcgcgat acggtcgcca cgtttcacct tctgtcccgg tttcaccaga
    32401 atcttgcgca agtgcatata acgcgtggtg tagctgcgac catgacgaat agccacataa
    32461 taacctgctg cgccactgcg tttggcaacc accacttcac cgtcacccac tgaaagcact
    32521 ggcgtaccct gcggcatggc gaaatcaaca cctctgtgtg gtgcaacgcg accggtcacc
    32581 ggattagtac gacgcgggtt aaagttagac gagatacgga actgtttcgc cgtcgggaat
    32641 cgcaagaatc ctttcgccag accagtaccg ttacgatcgt agaatttgcc atcttcagcg
    32701 cggatagcgt aataatcttt accttctgaa cgcaaacgta cgcccagcag ctggctttgc
    32761 tcacgtttac catcaagcat ttctcgagac attaataccg caaattcatc gccttttttc
    32821 agtttgcgga aatccatttg ccactgcatg gctttaatca ctgcgctcac ttcggcgctg
    32881 gttaaaccgg cgtttctggc gctggcaaca aagctccccc cgacggtacc tttcagcaga
    32941 ttgttaaccc actctccttg ctgcatttcg ctggtcattt taaaaccgtt agcggcagta
    33001 cggtcatagg ttcgggtttc acgacgagac acttcccagg tgaggcgctg cagttcgccg
    33061 tccgcggtta atgtccagga gagttgttga ccgattttca ggttacgcaa ttctttgtcg
    33121 gccgcagcca gttgggtgat atcacccata tcaataccat actgattgag aatgctgctt
    33181 agcgtatcgc cagtggaaac aacatattca tgcacgcccg cttcaccggc gattttgtca
    33241 tccagttcgt cctggggaat ggcttcatct tcttgtgcag cttgatcaat cggctcactg
    33301 gcttcaggta agagcgaacg aatttcgttc tgttccagct caatggtttt gacaattggc
    33361 gtggcatcgc ggtgataaac atagggccgc cagacagcga cggccagagt aagaacggtg
    33421 agcgacccca acataacgcg gtgtggtcgc ggtaaattat taaacgccag ggcgacagag
    33481 cgggctatct gttgcacgta atcacttcct cattaatctc ctttcaggca gctcgcatac
    33541 tggttggcta attgactcag gaattctgaa tagcttgttt tacccagttt gatattcgtc
    33601 cccaggggat ccaacgttcc catacgaacg gatgtccctc gtgcgacgct ctcaacgacc
    33661 gctggcctga actgtggctc agcaaaaacg caggttgctt tttgctcaac caactgtgtt
    33721 cttatttcat gtaaacgctg cgcgccaggt tgaatctcag ggttaacggt aaaatgacca
    33781 agcggtgtca gtccgaactg tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa
    33841 cctttcccct tgagcggcgc gagctcgtta ccaacctgcg tttcggttga ggctaattgt
    33901 gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac tttgcggcat aagttccact
    33961 aattttccat ggattgcaac cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc
    34021 atgttgaaat cgccgtgatg gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca
    34081 tcatcgccgt gaatactttt catcagtagc ggtttcacat cttctagctg cgcaatcgtt
    34141 acctgcttcg cttctggtaa tttgcttacc ggtttttgca taaacgcttc catctccggg
    34201 ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta catccgatgg acgcagtgaa
    34261 taatcatgtt ctgaagcacc gtcaggcagt aaaacctccg tttctgttac cccatcagca
    34321 atggcagaag cgatgaaccc aacgggttta agcgaagcga caacggcggc atctgcggcc
    34381 tgtgttgcac ctccccagag agcggcggat aatgctgcga aaagaagcgt ttttttatgt
    34441 aacataatgc gaccaatcat cgtaatgaat atgagaagtg tgatattata acatttcatg
    34501 actactgcaa gactaaaatt aacatgacaa gtctggtttc cctggaaaat gtctcggttt
    34561 cttttggcca acgccgcgtc ctctctgatg tgtcgctgga acttaaacct ggaaaaattt
    34621 tgactttact tgggccaaat ggcgcaggta agtcgacact ggtacgggta gtgctcgggc
    34681 tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa actgcgcatc ggctatgtac
    34741 cgcagaagct gtatctcgac accacgttgc cactgaccgt aaaccgtttt ttacgcttac
    34801 gccctggtac acataaagaa gatattttgc ctgcactgaa acgtgtccag gccgggcacc
    34861 tgattaacgc accgatgcaa aagctctctg gtggcgaaac gcagcgtgta ctgttagcgc
    34921 gagcattgtt aaatcgaccg caattattag tgctggatga acccactcaa ggcgtggatg
    34981 taaatggcca ggtggcgtta tatgacctta ttgaccaact gcgtcgtgaa ctggattgtg
    35041 gcgttttaat ggtttctcac gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt
    35101 gcctgaatca ccacatttgt tgttccggca caccggaagt tgtttccctg catccggagt
    35161 ttatttcaat gtttggtcct cgtggtgctg aacaactggg tatctatcgc catcatcata
    35221 atcatcgtca cgatttacag ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat
    35281 tgaattatta tttcccggtt ggttagccgg gatcatgctc gcctgtgccg cgggtccgct
    35341 gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc
    35401 attacttggt gtcgcgtttg gtttgttgct ggacgtgaat ccattctatg cggtgattgc
    35461 cgttacgctg ctgctggcgg gcggtctggt atggctggag aagcgtccac agctggcgat
    35521 cgacacgtta ttagggatta tggcgcacag tgccctgtcg ctgggcctgg tggtcgttag
    35581 tctgatgtct aacattcgtg ttgatttgat ggcttacctg ttcggtgatt tactggcagt
    35641 gacgccagaa gatctcatct ctattgcaat tggcgtggtc atcgtggtgg ctattttgtt
    35701 ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg gatctggcgt ttgttgatgg
    35761 tgtgaaatta cagcgagtga aattgttgtt gatgctggtg acggcattga cgattggtgt
    35821 agcgatgaaa tttgtcggtg cgttgattat tacttcgttg ctgattattc ctgcagctac
    35881 tgcgcgtcgc tttgcccgta cgccggaaca gatggctggt gtcgctgttt tggtggggat
    35941 ggtggcagta actggcggtt taaccttttc cgcgttttac gatacgccgg cgggtccgtc
    36001 ggtggtgcta tgtgcggcac tgttatttat tatcagtatg atgaaaaagc aggccagcta
    36061 atctatcgct gaacacattt gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg
    36121 ctatctctgg taggcctggt aagacgcgaa cagcggcgcg agcgcctttt ccgccctacg
    36181 gttcgctatc tctggtatat gcctgataag acgcgaatag cgtcgcatca ggcacactgc
    36241 caatgtcgga tgcggtgcga gcgaccaatc cgatttacgg catttctggc ggcgttatgc
    36301 caaagtgatt ccacgcccgc gtcgtcgcca tacgcccgcg cggtgtacgc tgcaaaaagc
    36361 cttgctgaat caaataaggt tccagcacat cctcaatggt ttcacgttct tcgccaatag
    36421 ccgccgccag gttatccaga cctaccggcc caccaaagaa cttatcgatt accgccagca
    36481 acaatttgcg gtccatataa tcgaaacctt cagcatcgac attcaacata tccagcgcct
    36541 gagcagcgat atctgccgag atggtgccat cgtgcttcac ttcggcgaaa tcacgcactc
    36601 gacgcagcag acggttggca atgcgcggcg taccgcgagc gcgacgagca acttccagcg
    36661 cgccgtcatc actcatctca agccccataa agcgtgcgct gcgactgacg atatattgca
    36721 gatccggcac ctgataaaac tccagacgtt gcacaatacc aaaacggtcg cgcaacggtg
    36781 atgtcagcga acctgcgcgc gtggttgcac caatcagggt aaacggcggc aaatcaattt
    36841 taatggagcg tgccgccgga ccttcaccaa tcatgatatc cagttggtag tcttccattg
    36901 ccgggtacag cacttcttca acaactggcg ataaacggtg gatctcatca ataaacagca
    36961 cgtcatgcgg ttcaaggtta gtgagcatcg cagccaaatc gcccgccttt tccagcaccg
    37021 gaccagaagt cgtgcgtaaa ttaacgccca tttcattggc gacaatgttg gcaagcgtag
    37081 ttttacccaa ccccggagga ccaaaaatca acaaatgatc gagggcatcg ccgcgcagtt
    37141 tcgctgcttt gatgaaaatc tccatctgtg aacgaacctg cggctgacca acatactctt
    37201 ccagtaattt agggcgaatg gcgcgatctg ctacatcttc cggcaaagtg gtaccggcag
    37261 aaatcagacg gtctgcttca atcatccttt acctcataac gcggcgcgta gggcttcgcg
    37321 aattaatgtt tcactgctgg cgtcagggcg agcgattttg ctcaccatgc ggcttgcttc
    37381 ttgtggttta tagcccagcg ccaccagcgc ggcaaccgct tcttgttcag catcgtcggt
    37441 cgccgggctg gcaggagacg tgagtaccag gtcggcggct ggcgtaaaga gatcgccatg
    37501 caaaccttta aatcggtctt tcatttcaac aatcaagcgt tcggcggttt ttttgccaat
    37561 acccggcagt ttcaccagtg cccccacttc ttcacgctca acggcattaa cgaactgctg
    37621 cgctgacatt ccggagagga tcgccagcgc caacttcggg ccgacgccgt tggttttgat
    37681 caactcttta aacaatgtgc gctcttgttt attgttaaaa ccgtacagca gttgcgcgtc
    37741 ttcacgcacc acaaagtggg tgaagacgat cgcttcctga cccgcttcag ggagttcata
    37801 aaaacaggtc atcggcatat gcacttcata gcctacgccg cccacttcaa ttaacaccag
    37861 cgggggttgt ttttcaatga tgatgcctct gagtctgcct atcacatgac gctcctgcgt
    37921 aatgaatcaa agataatgct gtatgataaa aaaatgctgg atagatatcc agcgaaggat
    37981 gaagaaaact tgcgaggtgt ctcgatgaac tgaaaaatgg cacagtataa tttattctac
    38041 aggtta
//
LOCUS       DAZGBC010000024        38024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGBC010000024 DAZGBC010000000
VERSION     DAZGBC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.024"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="ACHK1J_001291"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="ACHK1J_001291"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            120..195
                     /locus_tag="ACHK1J_001292"
     tRNA            120..195
                     /locus_tag="ACHK1J_001292"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Thr,seq:ggt)
     gene            235..350
                     /gene="rrf"
                     /locus_tag="ACHK1J_001293"
     rRNA            235..350
                     /gene="rrf"
                     /locus_tag="ACHK1J_001293"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(413..1171)
                     /gene="yhdZ"
                     /locus_tag="ACHK1J_001294"
     CDS             complement(413..1171)
                     /gene="yhdZ"
                     /locus_tag="ACHK1J_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO7910594.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1179..2282)
                     /gene="yhdY"
                     /locus_tag="ACHK1J_001295"
     CDS             complement(1179..2282)
                     /gene="yhdY"
                     /locus_tag="ACHK1J_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7910595.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2292..3473)
                     /gene="yhdX"
                     /locus_tag="ACHK1J_001296"
     CDS             complement(2292..3473)
                     /gene="yhdX"
                     /locus_tag="ACHK1J_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7910596.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3541..4566)
                     /locus_tag="ACHK1J_001297"
     CDS             complement(3541..4566)
                     /locus_tag="ACHK1J_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7910597.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4997..5218)
                     /locus_tag="ACHK1J_001298"
     CDS             complement(4997..5218)
                     /locus_tag="ACHK1J_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910598.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5471..8575)
                     /gene="acrF"
                     /locus_tag="ACHK1J_001299"
     CDS             complement(5471..8575)
                     /gene="acrF"
                     /locus_tag="ACHK1J_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO7910599.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8587..9744)
                     /gene="acrE"
                     /locus_tag="ACHK1J_001300"
     CDS             complement(8587..9744)
                     /gene="acrE"
                     /locus_tag="ACHK1J_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO7910600.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10143..10805
                     /gene="acrS"
                     /locus_tag="ACHK1J_001301"
     CDS             10143..10805
                     /gene="acrS"
                     /locus_tag="ACHK1J_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO7910601.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10808..10987)
                     /gene="yhdU"
                     /locus_tag="ACHK1J_001302"
     CDS             complement(10808..10987)
                     /gene="yhdU"
                     /locus_tag="ACHK1J_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO7910602.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11071..11955)
                     /gene="yhdJ"
                     /locus_tag="ACHK1J_001303"
     CDS             complement(11071..11955)
                     /gene="yhdJ"
                     /locus_tag="ACHK1J_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7910603.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLSTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(12041..12337)
                     /gene="fis"
                     /locus_tag="ACHK1J_001304"
     CDS             complement(12041..12337)
                     /gene="fis"
                     /locus_tag="ACHK1J_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO7910604.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12363..13328)
                     /gene="dusB"
                     /locus_tag="ACHK1J_001305"
     CDS             complement(12363..13328)
                     /gene="dusB"
                     /locus_tag="ACHK1J_001305"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO7910605.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13657..14538)
                     /gene="prmA"
                     /locus_tag="ACHK1J_001306"
     CDS             complement(13657..14538)
                     /gene="prmA"
                     /locus_tag="ACHK1J_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO7910606.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMTDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14550..16001)
                     /gene="panF"
                     /locus_tag="ACHK1J_001307"
     CDS             complement(14550..16001)
                     /gene="panF"
                     /locus_tag="ACHK1J_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO7910607.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(15991..16233)
                     /gene="yhdT"
                     /locus_tag="ACHK1J_001308"
     CDS             complement(15991..16233)
                     /gene="yhdT"
                     /locus_tag="ACHK1J_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO7910608.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16342..17691)
                     /gene="accC"
                     /locus_tag="ACHK1J_001309"
     CDS             complement(16342..17691)
                     /gene="accC"
                     /locus_tag="ACHK1J_001309"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO7910609.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17702..18172)
                     /gene="accB"
                     /locus_tag="ACHK1J_001310"
     CDS             complement(17702..18172)
                     /gene="accB"
                     /locus_tag="ACHK1J_001310"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO7910610.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(19150..20124)
                     /gene="yhdH"
                     /locus_tag="ACHK1J_001311"
     CDS             complement(19150..20124)
                     /gene="yhdH"
                     /locus_tag="ACHK1J_001311"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO7910611.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            20276..22216
                     /gene="csrD"
                     /locus_tag="ACHK1J_001312"
     CDS             20276..22216
                     /gene="csrD"
                     /locus_tag="ACHK1J_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO7910612.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPTTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            22521..23564
                     /gene="mreB"
                     /locus_tag="ACHK1J_001313"
     CDS             22521..23564
                     /gene="mreB"
                     /locus_tag="ACHK1J_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO7910613.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIDVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            23630..24733
                     /gene="mreC"
                     /locus_tag="ACHK1J_001314"
     CDS             23630..24733
                     /gene="mreC"
                     /locus_tag="ACHK1J_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO7910614.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            24733..25221
                     /gene="mreD"
                     /locus_tag="ACHK1J_001315"
     CDS             24733..25221
                     /gene="mreD"
                     /locus_tag="ACHK1J_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO7910615.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            25230..25823
                     /gene="yhdE"
                     /locus_tag="ACHK1J_001316"
     CDS             25230..25823
                     /gene="yhdE"
                     /locus_tag="ACHK1J_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO7910616.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            25813..27282
                     /gene="rng"
                     /locus_tag="ACHK1J_001317"
     CDS             25813..27282
                     /gene="rng"
                     /locus_tag="ACHK1J_001317"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO7910617.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27350..31150
                     /gene="yhdP"
                     /locus_tag="ACHK1J_001318"
     CDS             27350..31150
                     /gene="yhdP"
                     /locus_tag="ACHK1J_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO7910618.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            31489..32934
                     /gene="tldD"
                     /locus_tag="ACHK1J_001319"
     CDS             31489..32934
                     /gene="tldD"
                     /locus_tag="ACHK1J_001319"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO7910619.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGAMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(33062..33991)
                     /gene="aaeR"
                     /locus_tag="ACHK1J_001320"
     CDS             complement(33062..33991)
                     /gene="aaeR"
                     /locus_tag="ACHK1J_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO7910620.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            34174..34377
                     /gene="aaeX"
                     /locus_tag="ACHK1J_001321"
     CDS             34174..34377
                     /gene="aaeX"
                     /locus_tag="ACHK1J_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO7910621.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            34385..35317
                     /gene="aaeA"
                     /locus_tag="ACHK1J_001322"
     CDS             34385..35317
                     /gene="aaeA"
                     /locus_tag="ACHK1J_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO7910622.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            35323..37290
                     /gene="aaeB"
                     /locus_tag="ACHK1J_001323"
     CDS             35323..37290
                     /gene="aaeB"
                     /locus_tag="ACHK1J_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO7910623.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPINEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            37382..37654
                     /gene="yhcO"
                     /locus_tag="ACHK1J_001324"
     CDS             37382..37654
                     /gene="yhcO"
                     /locus_tag="ACHK1J_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO7910624.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(37710..37973)
                     /gene="yhcN"
                     /locus_tag="ACHK1J_001325"
     CDS             complement(37710..37973)
                     /gene="yhcN"
                     /locus_tag="ACHK1J_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO7910625.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         9378 a         9599 c         9865 g         9182 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa atttagcgtg
      121 ctgatatggc tcagttggta gagcgcaccc ttggtaaggg tgaggtcccc agttcgactc
      181 tgggtatcag caccactttt taggttaaag ttcggcagat tagaaaagaa tttgtctggc
      241 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
      301 gccgatggta gtgtggggac tccccatgcg agagtaggga actgccagac atcaaatcaa
      361 gcgaaaggcc atccgaaagg atggccttta tgcttttcga actaacattc aattaatgga
      421 ttacctgcga taaaaatgcc ctcgtacgct ctgacttagg atgcgcaaaa aactcatccg
      481 gtgcagcttg ctccactatt tcgccacgat ccataaaaat cacccggtca gcgacggttc
      541 gcgcaaaccc catctcatgg gttacacaca acattgtcat acccgattgc gccagcccaa
      601 tcatcgtatc cagcacctct ttcaccatct ccggatcgag cgccgatgtg ggctcatcaa
      661 acaacataat tttcggcttc atacacagcg agcgcgcaat ggcaacgcgt tgctgctgac
      721 cacctgaaat ctgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt
      781 aatgcatcgc cagagcttca gcctctttct ttggcatctt gcgtacccaa atcggtgcca
      841 gggtacagtt ctgtagaacg gtcagatgag ggaagagatt gaaatgctga aagaccattc
      901 ccacttcctg cctgacgcgc tcaatattgc ggatatcttc attaagttcg atgccatcta
      961 ccacgatccg tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt
     1021 taccggaacc tgaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta
     1081 tatttttcaa aacatggaat tgtccatacc atttattgac gttttccagc gtaatcatcg
     1141 cgttagcagg ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg
     1201 ttaaaacgtt tttccagatg ctggctatag cgcgacatgc tgaaacagaa gatccagtag
     1261 atcagcgcgg cgaaaacata cccttccgtc gacataccca accaggcggg atcaacggtt
     1321 gcctgctgaa cgctactgaa aagatcgaac aacccgataa tgatcaccag gctggtatct
     1381 ttgaagagtg cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc
     1441 agaataacca gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatat
     1501 tgccctttag gcagcgcctg taatccacct cgcacgactt ccgcaacata tgctgactga
     1561 aacaggatca cgccaaccag cgcgcggatc agtttgtcga tactggtgcc ttctgccata
     1621 aacaaaggca gcatgaccga agacataaac agaacggtaa tcagcggcac gccgcgccag
     1681 aattcgataa aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaac
     1741 gccagtaaga tcccccacgg tagcgccccc gcaatcccaa ctgatgcaat aattaaagtt
     1801 agcgtcagcc cgccccattg ccgggtttca acccgctcaa gagcaaaaaa accgccatac
     1861 atcagccacc agacaatcag tgggtaaatc actgcccaga ccgcaatata gcgaccgcga
     1921 tgcgggagta ttttccagaa cattggtgcg attgacacaa ggccaatcag taatgccagg
     1981 ttaattcgcc agcgttggtc gtgtgggtaa agtccataca taaactgacc aaatcgttcg
     2041 tggatgaaga cccaacaggc accggctttt gtgcagtcgg cacgcgttga gccaacccag
     2101 ttagcctgca aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg
     2161 cagccaatag tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc
     2221 atcgcgcggc ttgagttatg gctcgccggg cgcgagggat gagacagcaa tacttttgtc
     2281 atcatgactc cttagcgttc cacgatcgcg atgcggcggt tatagatatt catcagcagc
     2341 gagatagtca ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct
     2401 tgccccgtct ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata
     2461 gcggcggcaa gagaggagtt tttgacgatg ttgagatact ggctggttaa cggtggaata
     2521 atcacccgca gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaatccc
     2581 agtgaccgag ccgcttcatg ttgaccataa ggcactgcct ggatccccgc gcggataatc
     2641 tcggcgataa atgcagaggt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga
     2701 attaaaacca tcccgccgcg gaagttaaag ccttgtaggg ctgggacatc ccagtgtagt
     2761 gctgcgccaa aaagccattg cgccagtaaa ggcaaaccga tgatcaacac tgcggcgatg
     2821 ggccaggtgc ggcgcagctg tccggtcttt atctggtatg ttttattaaa gcggaatagc
     2881 ccaacagaaa ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc
     2941 agctgcggtg acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc
     3001 acagcctggc gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc
     3061 agcagcgggg gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     3121 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     3181 cacaatgcag aaaccagtag cgtattaagt aagccgacaa taaaaacgcg tccgtaggtg
     3241 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     3301 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt gtgaaataac
     3361 caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc
     3421 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3481 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3541 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3601 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3661 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3721 gccttcttta cccagcagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3781 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3841 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3901 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcagggcata
     3961 cagttgcgac tgatccgagg ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     4021 ttcgtcagag cgatcgaaag tcaccggtgt gtacttcata ttgtttgcct tgaaatagtc
     4081 ggcaacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     4141 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     4201 gacgccagta aatgccattc ccatcccggc gtcgcgagat gaagtccagg tcgtattacg
     4261 ggagagcaaa tccacttccc ctgactgtaa agccgtgaag cgttcttttg ctgtgagtgg
     4321 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaatatc
     4381 aacatcaata cctgaaaatt taccgtcagc atcggcataa gagaacccag gtaatccatc
     4441 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4501 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4561 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4621 tacctgtctg tggccatcct cttataagca aaagtagtgc catgttttgc gcacgatttc
     4681 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4741 aaaatgaaac atacgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa
     4801 taatgattgt tagcgtgagt atatttctgg tgggaagtgc gaaagttaaa ttgatgcgtt
     4861 atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc
     4921 agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac
     4981 tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag
     5041 ctgctgctcc gccagcaata gccccagccg tttcccagtt atcctgagtt gtgcctttca
     5101 tacaggtatt ggtgtgaacc agacgcccct gatcgtcata aaccggaacg cagggcgaat
     5161 cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg
     5221 tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa
     5281 tgccatttta tcaccggagg ttataaatct tacggaatga ataatgacaa attatgtgaa
     5341 ttgtaaaaat agtgaaggga gcgggaacta tagaaagttt ttaaagagtg agtggaagaa
     5401 tatcttatta atttttatgg gtttcactgg aaataataaa ggcgcccgaa agcgccttta
     5461 tgtttctgat ttatccttta aagcaacggc ggatcaccac aaagaacact ggtacgaaga
     5521 agattgccag caacgttgca gagaccattc cgcccattac cccgataccc actgcgttct
     5581 gcgcgccact gccggcaccg ttactgatag ccagcggtaa tacgccgaga ataaaggcga
     5641 gagaggtcat caggatagga cgcagacgca tacgtactgc cattagtgtc gcttcaacaa
     5701 cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt
     5761 tggccgacaa gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat
     5821 taaagagtgt cgccgccagc agcacgccga caatccctaa cggcacgacc aacataaccg
     5881 agacaggaat tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg
     5941 aaattgctac cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg
     6001 tccagtcata accaatgccc gcaggtaatt ttgacgcaag gttttccatc aacgccatgg
     6061 catcgccgga actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt
     6121 tgtagcgttc cagtcgcgga gagccataca cccaatgtga agtggtaaag gcagagaatg
     6181 gcaccatttc gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac
     6241 ggaatttggc atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt
     6301 taacgtaagt cccacccagc gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac
     6361 ctaatgcctg tgccttttcc tggtcaactt ccagtttaaa ctgcgcggtg tcttccaggc
     6421 cattaggacg cacgctgact aagctggcag gatgttgcgc cgccatacca agcaactggt
     6481 tacgggcctg ggttagggca tcgtgaccca gcccagcctg atcaattaac tcaaagtcga
     6541 aacccgttgc cgtgcccagt tcaacaatgg ctggcatgtt gaatggaatg acaaaaccgt
     6601 cgcggatctt gcccaattcc attttggcac gatggattac cgcttccgca ctgttttcgt
     6661 caccattacg ctcttcccac ggtttcagac tgacgaaggc cataccggcg ttttgtgcct
     6721 ggccgctgaa gctaaagccg ttaaccgtaa agacactttc aacgttcgct ttctcgttct
     6781 tcagatagta atccgtcact tgatccaaca ctttttgcgt ccgctcttgc gtcgcgccag
     6841 cgggtaactg aatcatggtc agaaagacac cctgatcctc ttcaggtaag aaggaagacg
     6901 gaagacgtaa aaacaacact accattcctg caacaatcag cgcatagatc agtaaatatc
     6961 gtcctgtgga accgaggatt ttgccgacgc tgttggtgta gtggttaacg ctatgatcga
     7021 aggtggtatt aaaccagccg aagaaaccgc ccttattttc gtgatgctca gcagagacgg
     7081 gtttaagcag cgttgcacat aacgcagggg taagaatcaa tgccaccaga acagaaagcg
     7141 ccattgccga aacgatggtg atagagaact ggcgataaat tgccccagta gaaccgccga
     7201 agaatgccat cggaataaat accgctgaca gcaccatcgc gatacccacc agtgcgccct
     7261 gaatttgcga cattgatttt tccgtcgctt cttttggcgg gagcttatcc tccatcatca
     7321 cgcgctcgac gttctccacc accactatcg catcatcgac gagcagcccg atggcaagca
     7381 ccatcccgaa catcgttagt gtgttgatgg agtaaccaaa agcggcgagg atggcaaacg
     7441 tccctaacaa caccacgggt accgcaatgg tggggatcag cgttgctcgc atattctgca
     7501 agaacagata catcaccagg aacaccagca taatagcttc gaacagtgtt tttaccactt
     7561 cgtgaataga aagctggacg aatggcgtgg tgtcataagg gtagagaacc ttcattccct
     7621 gcgggaagaa tggctgtaat tccgccagtt ttgccttaat ggctttcgcg gtatcgagag
     7681 cgttcgcacc ggttgccagc ttaataccca ggcccgccgc cggttttccg ttgatgcgag
     7741 cgataacgtt atagttttca ccgccaagtt caacccgtgc gacatctttc agacgcacca
     7801 ccgagccgtc actgtttacg cgcagggtca ctttgccgaa ttcttccgga ttttttaacc
     7861 gcgtctgagc aataatcgag gcattcaact gttgccctgg taacgctggc gttccgccca
     7921 actgtccggc agcgatctga tcgttctgta ccttcaactg gttaatcaca tcaaccggtg
     7981 tcagtttata tttgtttagc agatcggcgt ccagccagat acgcatcgca tactgtgcgc
     8041 cgaaaagctg tacgtcaccg acgccattca gacggctaag cgtatcttta acgttagagg
     8101 ccacatagtc cgagatatcg tcctgtgtgg tgtctgggtt atcagagaca aagcccgcca
     8161 ccatcaaata gctgctactg gacttttcaa cactgatccc ctgctgctga acctcctgcg
     8221 gcagcaacgg cgtggcgagc tggagtttgt tctgcacctg cacttgcgcg atatcaggat
     8281 cggtcccgga ctggaaggta agggtaattg tcacgctacc ggcggaatcg ctggtggagg
     8341 acatatacat caggttatcg ataccgttca tattctgttc gataacctgc gtcaccgtat
     8401 cctgcacggt ctgcgcatca gcgcccggat agtttgctga aacagaaacc gcaggcggtg
     8461 caatggttgg atactgagcg acgggcaatt gtaggatcgc cagtgcgccc gccatcatca
     8521 gaataatggc cagcacccat gcaaatattg gtcgtcgaat aaaaaagttt gccatgtcag
     8581 attaccttac ttcgatgcag tatctgcggg ggtatcggta gtggctttaa cctgctctcc
     8641 cggacgcgct ttttgcaggc cgctgacaat gacttgatcg ccagatttca gtccttcact
     8701 aatcaaccat ttatcgccaa tcgcctgact ggcaacgaca gggcgcgctt caacctgact
     8761 tttatcgtta acaatcagca cggttgcatc accacgcggt gtgcggctaa cgccttgttg
     8821 cgggataaga atggcgtcag gttggacgcc ttcatcaatc cgtgcacgca caaacatacc
     8881 cggcaaaagc gtatgttgcg ggttagggaa gacagcacgt agggttatgg agccggtgct
     8941 ttcatcaacg gtcacatcgg agaattgcag cgtacctttc aggggatagg tttgaccgtt
     9001 ttccatgacc aactctacgt tgctggtggc gttttcctta tgcaaatttc cttgctctac
     9061 tgattgcttc agcctcataa aatcgttgct ggattgggtc acatcaacgt agataggatc
     9121 gagctgctgg acagtcgcca gttcagtcgt ttgcccatta gtgacaagag cgccttcggt
     9181 cacagtcgat ttgccgatac gtccgctaat tggcgcagtc actttggtat aagcaagatt
     9241 gatgcgagcg ctttcgactg tggcttttgc ggcaatcacg gcggcatcgg cctggcgagc
     9301 atcagcaatg gcctggtcgt actcctgctg gctgatgtat ttcgtaccca cgagcggaac
     9361 gtaacgtttt accgtcaaat gcgcgatggc ggcggcggct tcacttttcg ccagttcgcc
     9421 tttcgcgctg tcataatttg cctgataggt cgcgggatcg atctggtaca gggactggcc
     9481 tgcttgcaca tcgctgcctt cagtgaaatt gcgattcagt acgatcccgc taacctgtgg
     9541 gcgaacttcg gctatacgat aagcattggt gcggcctggt aattcagtct taacttctaa
     9601 cggggccgtt tttacaatat gaacggtaac ctgcggttca ccgacgtgag ctttctcttc
     9661 tcccttatcg ttacaaccgg cgattaaagc cgcggagatc agaataaagg agggcaggag
     9721 gaaaaacctg gcatgtttcg tcattactat tcctcaaaaa accaaaagcg cgttatttac
     9781 ccaaaaaggc aacgcgttaa ctcgcagaaa gaaaaataca gttcgctatc ctacaaatta
     9841 tcattcgtcg atgtaaggaa tagttatgaa tacaggcgtc tcaaggcaca taaacacaaa
     9901 aaaagattaa tattctactg ttttattttg acgcgggttg aaagaggcag aattaaaacc
     9961 tcgtaaattg aaatatatat tgatgtagtg aatgtatctt aggtaaataa tatatattat
    10021 ttatttaacg atttatagca actcaatatt agcctctcgt ataaatacac attaggtgat
    10081 agattaacct tcgctatttt ctcactctgt gtcgaatata tttatttcct gaataattaa
    10141 tcatggcaaa aagaaccaaa gccgaagctc tgaagacccg gcaagaactg attgaaactg
    10201 ccatcgccca gtttgcgcag catggcgtaa gcaagacgac gctcaacgac attgccgacg
    10261 ccgctaacgt tacgcgtggc gctatctact ggcacttcga aaacaagact caactgttta
    10321 atgagatgtg gttgcaacag ccttcattgc gggagttaat ccaggaacac ttgacggctg
    10381 gattagagca tgacccgttt caacaattgc gtgaaaaatt gattgtcggc ttgcaatata
    10441 ttgccaaaat tccccgccag caggcgttgc tgaaaatctt atatcacaaa tgtgaattta
    10501 atgatgagat gctggccgag ggagtgatac gcgaaaagat gggctttaat ccgcagactc
    10561 tccgcgaagt attgcaggcg tgtcagcaac aaggttgtgt agcaaataac ctcgatttag
    10621 atgttgtgat gattattatt gatggtgcct tcagcggaat tgttcaaaac tggttaatga
    10681 atatggcggg ttatgatctt tataaacaag cccccgctct ggtcgataac gtattaagaa
    10741 tgttcatgcc agatgaaaac ataacgaaat taattcatca aacgaatgaa ttaagtgtca
    10801 tgtaaattca cattctgtcc tgaaaattca gttcgtcaca gttaaccagt tttagtggat
    10861 taactgagtt catattgcgg catttgtctg tttctgttgc cagcagttca atccactgca
    10921 tcagtaaagt atcaaacaga aaaacgaaga cagcgaaaac gacgagccac caatacttgc
    10981 gaatcattac ctgagcctgt gcatgtttaa ccaagtctgg caggaaatat acacgaaatg
    11041 aataatctga aaaccaggct tacacttcct ttactttgca atgagatcgg ggtcaacttc
    11101 gcttaaccga cttcgttttg acaggttgcc ggtctttctt tttttcactt tcgccagttc
    11161 ttccgcagag taatgcgacg cgacatccag ccgtcgaagc cccattttga tgtactcgct
    11221 gttgatctca ataccaatga attttcgccc gctggagacg gctacggcac cggtagtaaa
    11281 gctaccggca aacgggtcga gaacgatatc gcctgggttg gaagaggcaa gaataatgcg
    11341 tttcagtaag gcttccggtt tttgcgtcgg gtggttctca tattcatcca tcaaataacg
    11401 cacgcgcgga aaatcccaga cgttacccgg tactttttga tgattgtaag gctgtggagg
    11461 atttttgcga taatcgatca acgcgcgctg cgatccagtt ttggcttcta ccagaatagc
    11521 atcaccgttg aacgtgtagt tctttgcgtc tttcaccatc atcaggatgg gttcgtacat
    11581 ggaaccgtag tgttttttcg cctgcactcc agaactgtca tatgaccaga cgatgcgact
    11641 tttgatggta aaaagcttgc ggcactggag atcgataaag ggcatgtttt ccgtgctgtt
    11701 catgatgtac atgctgccct gctttttcag aacgcggtgg cactctgcaa tcacttcaaa
    11761 cagccagtcg ataaacagat cttctttcca ggcttcgatc agaccatcaa aatttttacc
    11821 gatgttatac ggtgggtcgg caaagatcag atcgacgctt tcagtagata gctttttaag
    11881 ttcggcaagg gcatcaccgt gaataatggt cttagcttca ttaccaaacc gggtcggttc
    11941 acatcctgtt ctcatggtca ctccctttgt gacacctata aaaaaggcgc ttccccatgc
    12001 cgagtagcgc ctttttaatc aagcatttag ctaacctgaa ttagttcatg ccgtattttt
    12061 tcaatttttt acgcagcgta ccacggttga tgcccatcat cagtgcagca cgggtctggt
    12121 taccacgggt gtattgcatc accatgtcca acaggggctg ttctacttca gccagtacca
    12181 gctcatagag gtcattcaca tcctgaccat tcagttgagc aaaatagttc ttcagtgcct
    12241 gtttaaccga gtcacgcagg ggtttttggg ttacctgatc ctgagagtta acggtagaaa
    12301 cggtcagtac gtcagaattt acgcgttgtt cgaacatagt tctgtcagct ctttatttct
    12361 gtttacgcaa aattttcgaa gtatgcctcc aacgcctcca gctgttcgct ggcatcctca
    12421 atggcgttga atgtgcgccg aaactggtca tttggagcgt gttcctggag ataccaggaa
    12481 acgtgtttac gtgcaattcg gtaccctttt gccggaccat aaaagtcatg cagttcccga
    12541 acgtgcgcgc aaagcaagcg cttaacctct gccaaaggca gcgggggcag caactcccca
    12601 gtgtccagat aatgctggat ttcccgaaag atccagggtc ttccctgagc tgcgcggcct
    12661 atcatcaggg catccgcccc tgtatagtcg agcacagctc tggctttaag cgggtcagta
    12721 atgtcgccat tcgcgataac cggaatggaa actttctgct taactgcccg aatactgtcg
    12781 tactcagctt ctccattgaa caaacaggcg cgtgtacggc catgaatggt cagagcctga
    12841 atgccacagt cttcagccag ttgggcaatc tcttcgcagt tacggtgttc cggcgcccag
    12901 ccggtgcgaa tcttcagggt aacaggaacg tccactgcat tgacgacctc ggtaaggatc
    12961 gatttaacga catccgggta ctgcaagagg gctgaacctg cgagcttgcg attcactttt
    13021 ttagccgggc aacccatatt gatatcaata atctgggcac cgctttccac gttaatacgt
    13081 gctgcatctg ccatttcttt cggatcgcta ccagcaattt gcacggtgcg aataccgggt
    13141 tcatcaatgt gcaccatccg taaacgagat ttgtcgcttt cccaaacctg tgggttagaa
    13201 gacatcatct cggatactgt caatccggct cccatctcgt agcacaacgt ccgaaaaggt
    13261 ctgtctgtaa tgccagccat gggcgctgcg atcaggcgat ttctgagctg atattgtccg
    13321 atgcgcatga gttaagaaat gaccatactg tgactgcaag gcggcgtata ttacgcattt
    13381 tttgcacgag atgaaaggcc aaactttgac caatcctctg agatggatca aagaattgca
    13441 tttaaaatga gcgtggtgcg ataattactc ataaaaatca tcatattaga aaatagtgac
    13501 taaaatttac actcaaagaa atttgagtaa gttctcaatt tttctttatg aatgaaaatt
    13561 ttggcacgca aatttgcgta aataatcggc aaatttacgt gccttttgtg agcttgctcg
    13621 cacttcgccc cgcgtcaccc tacggcgatg cgaaggttaa ttcttacgac cggtaatacg
    13681 gcaccactct tctttttcca cgaccgggtc cagtgcgaag ctgtcggcat aagcttcaca
    13741 aacgctctct gcctggcttg ccagaatacc ggaaaggccc agcaaaccgc ctgaaaccgg
    13801 caggacgctg attaacggtg ccagttcacg taatgggcct gcaaggatgt tagcgaccac
    13861 cacgtcggct ttcatttctt ctggctgatc tttcggtaag tagagttcca gacggtcaga
    13921 aacgccatta cgttcggcgt tatcgcggct ggcctgaatc gcctgcggat cgatatcaat
    13981 accaatggct tttgctgcac ccagtttcag cgccgcgatc gccagaatac cggaaccaca
    14041 gccaaagtcg atgactgttt taccggttaa atcgaggctg tcgagccatt gcaggcacag
    14101 agaggtggtt ggatgggtac ccgtaccaaa cgccagccct ggatctaaca tcacgttgac
    14161 ggcgttttcg tccggcacat cacgccagct tgggcagatc cacagtcgtt caccaaagcg
    14221 catcgggtgg aaattatcca tccattcgcg ctcccagtct ttatcttcca gttgttcgat
    14281 tttatgcgcg aagcctgcgc cgagcagcgg atggttttcc agaatcgcca ccacgtcggt
    14341 catatcggtt tcagcgtcga acagaccaat cacatcggtg tcgccccaca ggcgcgtttc
    14401 gcccggcagc ggctcaaata ctggcgtatc gtgggtgtcc tgaaaagtga tagaaacggc
    14461 acccgcttcc atcagcgcat cgctaagatc ttccgcgttc gcgccggtgg tgttcagttt
    14521 cagttggatc caaggcatgg caaaactctt tatttatcag tagtcaaaac ggtagcttgc
    14581 gggacggatg taccgaaacg gtttccgacc aggaaagcca gcaaacttag tagtaacgag
    14641 ggcacgatag ggtggaagcc caggtactga atattcagcg tcgcgagtac ggcatacagc
    14701 acgccgccaa cgatcatcgc acttagcgcg cctttggcgt tggcgcgttc ccagtaaaga
    14761 cccagcacca gcggccacag gaaaacggct tccagcccac cgaaggccag caaattcagc
    14821 cagatgatca tttctggcgg cttccaggcg gcaagcagca gcaacgcgcc gagaactaac
    14881 gtaattaccg ccgacatccg cttcagacgc gtctcgtttt gcatttgatc cggacggata
    14941 ttcagataga gatctttaat gatcgtagcg gaactttgta gcaattgggc gttaattgtc
    15001 gacatgatcg cagccatagg tgcagccaga aagatcccgg cagcaaacgg tggcagcact
    15061 tttaccatta acgtcgggat caccagatcc ggtacggtga gatcggggat cactgcacga
    15121 cctaacgctc cggccaggtg cataccgaac atcaggattg cgactacaat cgtaccgatg
    15181 atgatccccc ggtgtactgc tttgctgtct ttataagaga tacagcgcac cgcagtatgc
    15241 ggcaggccta taacgccaaa gcacaccagt acccagaacg acgtcataaa ggcaggcgac
    15301 agaatatcgt cagcgccttg tggcgtaacc agttgcggat cgatggtttg caaggtctgt
    15361 actgcgttgc ttaagccgcc agcggcatgt accacgccaa taagcagcac aacggtgcca
    15421 atcagcatca caagcccttg catggtgtcg ttcagcacgc tggcgcgaaa gccaccaaat
    15481 gcggtatata acgcaatgct gataccaaaa atcagcagcc cggtttcata aggaataccc
    15541 gccgcggttt ccagcaggcg cgcgccgccg ataaactgca cggtcatcgc gccaataaac
    15601 gcgaccagca aactcaaact cgccagccac accaaaagac gactctgata gcgggcaaac
    15661 agcatatcgt tcagcgtcac tgcattgtag cggcgcgcaa gaatcgcaaa cttcttgccg
    15721 agaataccga gtgaaagcca gactgcagga agctgaatca tcgccagcaa tacccagcca
    15781 aggccgtatt tgtaagcagc tccaggcccg ccgataaacg aactggcact gatataagtc
    15841 gcggtgagcg tcatcgccag cacaataccg cccatagagc ggctgccgag gaaatactca
    15901 ttaaggaagg tgccggtgct ccgtttacgc atcgcataaa ccgagatacc gaacaccacc
    15961 accagatagg cgaccagcgg tagaattact tcaagctgca tcgtcatcct ccagcgggat
    16021 atcgcgatag ataaatttca ccatcgccca gcacagtcca ataaacagca gcggcgtcag
    16081 gatgcaggcc atctcaaacc agcgcggaaa gccggtaaaa ccgggggcaa cgccaggtaa
    16141 gtaagcggct gctaaccaaa ctgccagata caaaagggtc agccccagcg cccagcgcgc
    16201 ctctttatgg gcctgaacaa aacgagtgtc cattttttgt ccctgatggg tgaagaaagc
    16261 gaggattgta ccttatgggg gttgtcgatc cccagtaata aaaaaggccg gaaaatccgg
    16321 ccttttgacg cttttgcagt cttatttttc ctgaagaccg agttttttct ccagatagtg
    16381 gatgttagtg ccaccatgct ggaagttctc gtcattcatg atgcggatct gcagatcaac
    16441 gttggttttg ataccgtcga tgatcagctc ctgcagcgca tttttcatgc gggcaatcgc
    16501 cacgtcacgg ttttcaccgt agcaaatcag cttaccgatc attgagtcat agtacggcgg
    16561 tacggtgtag cccgcgtaga tatgagactc ccaacgtacg ccaaaaccgc caggtgcgtg
    16621 gaaacgggtg attttgcccg gacttggcag gaaggtgttc ggatcttcgg cgttgatacg
    16681 acattccacc gcatggccgc gaacgtgaac ttcttcttgc ttgatcgaca gcggttgacc
    16741 ggcagcgata cgcagctgtt ctttgatcag gtcaacgccg gtgatcattt ctgtaaccgg
    16801 gtgttctacc tgaatacggg tgttcatttc gatgaaatag aactcgccgt tttcgaacag
    16861 gaactcgaaa gtacctgcac cgcgatagcc gatatcaaca cacgctttag cgcaacgttc
    16921 gccgatgtag cgacgcagtt ccggggtaat gcccggtgct ggtgcttctt cgaccacttt
    16981 ctggtggcgg cgctgcatgg agcagtcacg ttccgccaga tagatagcgt tgccctgacc
    17041 gtcagccagt acctgaatct cgacgtggcg aggattttcc aggtatttct ccatgtaaac
    17101 catatcgttg ctgaaagcag ctttcgcttc cgcacgggtc atggagatgg attgtgccag
    17161 ttcagcgtcg ccgcgcacta cgcgcatacc gcgaccaccg ccgccgccgg aggctttgat
    17221 aatcaccgga taaccaatgc gtttagcaat ggcacggttt ttatccatat cgtcgcccag
    17281 cgggccgtca gaacccggta cgcaagggac gcccgctttt ttcatcgccg cgattgcgga
    17341 tactttgtcg cccatcaggc gaatggtttc tgctttcggg ccaatgaaga taaagccgga
    17401 gcgttcaacc tgctcggcaa agttggcgtt ctcggagagg aagccgtaac ccggatggat
    17461 tgctactgcg ccggtgattt cagcggcgct gatgattgcc gggatgttca gataactttt
    17521 tactgacggt gcagggccaa tacagaccgt ttcatctgcc agtaatacgt gttttagatc
    17581 gcgatccgcg ctggagtgca cagcgacagt cttgatgccc agttctttac aggcacgaag
    17641 aatacgcaat gcaatctcgc cgcggttggc aataacaatt ttatccagca tgttcgcctc
    17701 gttactcgat gacgaccagc ggctcgtcaa attctaccgg ttgtccactt tcgaccagaa
    17761 ttgctttcac ggtaccggat ttgtccgctt cgatctggtt catcattttc atggcttcaa
    17821 cgatgcacag ggtatcgccc acgttgactt tctgacccac ttcgatgaac gcttttgcgt
    17881 ccgggcttgg ggtgcggtag aaagtaccaa ccatcgggga acgtacgatg tgaccactga
    17941 tttccgctgc tgctggcgct tccatggaag gaacggtcgc cggagcggct gcgttagatt
    18001 gagctggctg ctgcatcatt ggtgcagcgt aagcttgttg catcacaggg aaacttgcgg
    18061 caggagctgc acggctaatg cgtactgact cttcgccttc agaaatttcc agttcggaga
    18121 tgcctgattc ttcaaccagc tcgatcagtt ttttaatctt acgaatatcc atgagtgggt
    18181 tccgtactct ttgtttagtg tgattgtgac aggcgtttca ccgccgtctg taaagcgtat
    18241 gccccgacag ctgtatgcat agcgataaat tccagcaggc cgggacgccg gtctattttg
    18301 ccccagagtg tcaatgttag acttgacgga cattgtgcag cgatctgctg ctatcctccg
    18361 gcaaaaaaca aaatatacct tcgttgcgcc aatgtcacct tttccgcagc gcaaaaactg
    18421 cgtcagggag gacgaggtcg cacattataa cgatttcgta gcaattggca gctaaatact
    18481 ggtcttatca gggaagataa tcaacagcta acatgtaaat aaccttcaat accgtgtaat
    18541 ttgcaacaag ccgcacaatt cacgaaatta gcgccatcat cgacggaact tcttataacg
    18601 taaggtgaaa agctgtaaag ccagccctgc gaagatgagg ggctgcaatg agataatctt
    18661 cacagaccac aaataatgta gggatgccag gatcgcgaca agatagacga cattacgcag
    18721 cggttgctgg ggattaccca attttcactg catcgtctgg atagatgtga atgctaaagc
    18781 acgcaaaatt aaccgattga caatgctcag cattaaatag gttcgagtga ttaatttgta
    18841 gtcaaacaaa atcagattat tcaccccatg gtaccagaaa taagtaaggg gttaagcgta
    18901 gcgtcgccca ggcaaagcac cataaaccta acaggcgacg gaggcgtatc aataatagct
    18961 gtttagtgga gtatcaatgc gcatcaattt atggtcagtc gaaatcatga ctttccagtt
    19021 tggatgatgt tgtacaactt catgaccaag gtgttctacg aaagctgtcc ggctaaccgc
    19081 ttttcccgtt aaccgattga ataatgattt caaaatgagt tccctctctt attattcctt
    19141 gctaaatggt tagttaacct tcaccagcgt gcgaccttgg atctggttat taatgatggc
    19201 ctcggcaaag ttcggtgcct ctgacagaga tatctctttt gccgcctggg tatagaatga
    19261 ttccggtaaa tcggcgacca gtcgctgcca ggcttgtgcg cggcgttctg gtggcgtcat
    19321 tactgaatcc accccttgca aacggacatt acgcagaata aatggcatga ccgtggttgg
    19381 cagagtaaaa ccacccgcca gaccacaggc cgccacgcag ccgccgtaat tcatttgcgc
    19441 cagcactttt gccagcactt tgtcgccaac agtgtcaatt gccccagccc agacttgttt
    19501 ttccagagga cgggattcgg caaactcatc acgagggaga atacggctgg cacctaaact
    19561 tttcagatat tcatgggtac tttcgcgacc ggaaacggca acgacctgat aacccaactt
    19621 atgcagcagc gccacggcgg tactgccgac gccaccactg gcacccgtca cgacaatctc
    19681 cccgtcctgc gggcgaacac cggcatcttc cagcgccatc acacacagca tggcggtaaa
    19741 accggcagta ccgataatca ttgctttacg cgcgtccagc ccttgcggca tggcaaccag
    19801 ccagtcacct ttcactcgcg cctgctccgc cagcccaccc cagtggtttt caccaacgcc
    19861 ccagccagtg agtaacacct cctgaccggc atgaaaacgc ggatcttcgc tggtgcgtac
    19921 agttccggca aaatcgatcc caggaatcat cggaaaatta cggatgattt ttcccttacc
    19981 ggtaatcgcc agcgcatctt tatagttcag gctcgaccag tgaacatcga ccgtgacatc
    20041 gccctccggc aggcgacttt cgtccagagt ctgtactgat gcgagagttt tgccgtcctg
    20101 ctgttctaaa agtaacgcct gcataagtgg tcctcatgtg catgatggat tggaaaataa
    20161 tttctgaaga ctatactcgc taatggaaaa gcaaagccga tgacgcgcaa taaattgcga
    20221 gataaatctg atttgctagt atgcccgctt cctcactatc ggagttaaca caaggatgag
    20281 attaacgacg aaattttcgg cctttgttac gctgctcacc gggttaacaa tttttgtgac
    20341 tttgctgggc tgttcgctaa gtttctacaa cgccattcag tataagttta gtcatcgcgt
    20401 tcaggcggtg gcgacggcga ttgataccca ccttgtgtcg aatgacttca gcgcattaag
    20461 gccacaaatt accgaattaa tggtgtcagc agatatcgtt cgtgtagacc tgctgcatgg
    20521 tgataaacag gtttataccc tggccagaaa tggtagttat cgtccggttg gcaccagcga
    20581 tctgtttcgt gaactgagcg ttccgttgat aaagcatccg gggatgtcgt tgcgtctggt
    20641 ttatcaggat ccgatgggca actatttcca ttcgttgatg accaccgcgc cgctcacggg
    20701 ggcgattggc tttatcattc ttatgctctt tctggcggta cgctggttac aacggcaact
    20761 tgccgggcaa gaattgctgg aaacccgggc tactcgtatc ttaaacggtg agcgtggctc
    20821 taatgtgttg ggaaccatct atgaatggcc gcccacaacc agcagtgcgc tggatacgct
    20881 gcttcgtgaa attcagaacg cacgcgaaca acacagccgt cttgatacgc tgatccgctc
    20941 ttatgccgca caggacgtga aaaccggcct caataaccga ctctttttcg ataatcagtt
    21001 agcaacgtta ctggaagatc aggagaaagt aggtacccac gggatcgtga tgatgattcg
    21061 tctgccggat ttcaatatgt tgagcgatac ctgggggcac agccaggttg aagaacagtt
    21121 cttcactctg acgaatctgc tgtcgacatt tatgatgcgc taccctggcg cactgctggc
    21181 gcgttaccac cgcagtgatt ttgctgcgct gttaccgcac cggacgttaa aagaggcaga
    21241 gagcatcgcc ggtcagttaa tcaaagccgt cgataccttg ccgaacaata aaatgctcga
    21301 tcgcgacgat atgatccaca ttggtatctg cgcctggcgt agtggtcagg ataccgagca
    21361 ggtaatggaa catgcagagt ctgccacgcg taatgcggga ttgcagggcg gcaatagctg
    21421 ggctatttac gatgactcgt tgcctgaaaa aggacgcggt aatgttcgct ggcgtacgct
    21481 tatcgagcaa atgctcagtc gcggcggccc gcgcctttat caaaaaccgg cggttactcg
    21541 cgaaggtcag gttcatcatc gcgaactcat gtgccgcatc tttgatggta atgaagaggt
    21601 tagctcggca gagtatatgc cgatggtctt gcagtttggc ttatcggaag agtatgaccg
    21661 tctgcaaatc agccgtctta ttccactatt gcgttactgg ccagaggaaa atctggcgat
    21721 tcaggttacc gttgagtcgc tgattcgccc gcgttttcag cgttggctgc gcgatacgtt
    21781 aatgcaatgt gaaaaatcac aacgaaaacg cataattatt gaacttgcag aggccgatgt
    21841 aggtcaacat atcagtcgtt tacaacctgt tattcgttta gtgaatgctt taggggtacg
    21901 ggtagccgtc aaccaggctg gtttgacgct ggtaagcacc agttggatca aagaacttaa
    21961 tgttgagtta ctcaagctcc atccggggct ggtcagaaac attgagaagc gaacggagaa
    22021 ccagctgctg gttcaaagcc tggtggaagc ctgctcaggg accagcaccc aggtttacgc
    22081 caccggtgtg cgttcgcgaa gcgagtggca gaccctgatt cagcgcggtg ttacaggcgg
    22141 gcaaggggat tttttcgcgt cctcacagcc acttgatact aacgtgaaaa aatattcaca
    22201 aagatactcg gtttaacctg ccgtttaatc cgttttcacg tagaataacg cgcgctgcgt
    22261 ctcatgggag tgtgcttgtc tgctcgccag attgttgcag cacatatgca gatgaatgac
    22321 cttacgcggt tgcaaacagg cgaggaatgc tgctgatgca ttaagccttt ctggactcag
    22381 gcagagattt gtaacaaagg aaacgaactg cactaatttt caccgtagca gatgattttt
    22441 gcgccttgtc gctgctgcgt gtggttggta aagtaagcgg attttctttt ccgccccagc
    22501 tttcaggatt atcccttagt atgttgaaaa aatttcgtgg catgttttcc aatgacttgt
    22561 ccattgacct gggtactgcg aataccctca tttatgtaaa aggacaaggc atcgtattga
    22621 atgagccttc cgtggtggcc attcgtcagg atcgtgccgg ttcaccgaaa agcgtagctg
    22681 cagtaggtca tgacgcgaag cagatgctgg gccgtacgcc gggcaatatt gctgccattc
    22741 gcccaatgaa agacggcgtt atcgccgact tcttcgtgac tgaaaaaatg ctccagcact
    22801 tcatcaaaca agtgcacagc aacagcttta tgcgtccaag cccgcgcgtt ctggtttgtg
    22861 taccggttgg cgcgacacag gttgaacgcc gcgcaattcg tgaatccgcg cagggcgctg
    22921 gtgcccgtga agtcttcctg attgaagaac cgatggctgc cgcaattggt gctggcctgc
    22981 cggtttctga agcgaccggt tctatggtgg ttgatatcgg tggtggtacc actgaagttg
    23041 ctgttatctc cttgaacggc gtggtttact cctcttccgt acgcattggt ggtgaccgtt
    23101 tcgacgaagc tatcatcaac tatgtgcgtc gtaattacgg ttctctgatc ggtgaagcca
    23161 ccgcagaacg tatcaagcac gaaatcggtt cggcttatcc gggcgatgaa gtccgtgaaa
    23221 tcgacgttcg tggccgtaac ctggcagaag gtgttccacg cggttttacc ctgaactcca
    23281 acgaaatcct cgaagcactg caggaaccgc tgaccggtat tgtgagcgcg gtaatggttg
    23341 cactggaaca gtgcccgccg gaactggctt ccgacatctc cgagcgcggc atggtactca
    23401 ccggtggtgg cgcactgctg cgtaaccttg accgtttgtt aatggaagaa accggcattc
    23461 cagtcgttgt tgctgaagac ccgctgacct gtgtggcgcg cggtggcggc aaagcgctgg
    23521 aaatgatcga catgcacggc ggcgacctgt tcagcgaaga gtaatcggat gcaggcaggg
    23581 gaagcgtctg tttaccctgc ctggtctgat acgagaatac gcataactta tgaagccaat
    23641 ttttagccgt ggcccgtcgc tacagattcg ccttattctg gcggtgctgg tggcgctcgg
    23701 cattattatt gccgacagcc gcctggggac gttcagtcaa atccgtactt atatggatac
    23761 cgccgtcagt cctttctact ttgtttccaa tgctcctcgt gaattgctgg atggcgtatc
    23821 gcagacgctg gcctcgcgtg accaactaga acttgaaaac cgggcgttac gtcaggaact
    23881 gttgctgaaa aacagtgaac tgctgatgct tggacaatac aaacaggaga acgcgcgtct
    23941 gcgcgagctg ctgggttccc cgctgcgtca ggatgagcag aaaatggtga ctcaggttat
    24001 ctccacggtt aacgatcctt atagcgatca agttgttatc gataaaggta gcgttaatgg
    24061 cgtttatgaa ggccagccgg tcatcagcga caaaggtgtt gttggtcagg tggtggccgt
    24121 cgctaaactg accagtcgcg tgctgctgat ctgtgatgcg acccacgcgc tgccaatcca
    24181 ggtgctgcgc aacgatatcc gcgtaattgc agccggtaac ggttgtacgg atgatttgca
    24241 gcttgagcat ctgccagcga atacggatat tcgtgttggt gatgtgctgg tgacctccgg
    24301 tctgggcggt cgtttcccgg aaggctatcc ggtcgcggtt gtctcttccg taaaactcga
    24361 tacccagcgc gcttatactg tgattcaggc gcgtccgact gcaggactgc aacgtttgcg
    24421 ttatctgctg ctgctgtggg gggcagatcg taacggcgct aacccgatga cgccggaaga
    24481 ggtgcatcgt gttgctaatg aacgtctgat gcagatgatg ccgcaggtat tgccttcgcc
    24541 agacgcgatg gggccaaagt tacctgaacc ggcaacgggg atcgctcagc cgactcctca
    24601 gcaacccacg acaggaaatg cagctactgc gcctgctgcg ccgacacagc ctgctgctaa
    24661 ccgctctcca caaagggcta cgccgccgca aagtggtgct caaccgcctg cgcgtgcgcc
    24721 gggagggcaa tagtggcgag ctatcgtagc cagggacgct gggtaatctg gctctctttc
    24781 ctcattgcgc tgttgctgca aatcatgccc tggccggaca acctgattgt tttccggcca
    24841 aactgggtgt tactcatctt gttgtattgg atcctggcct tgccccatcg cgtaaatgtg
    24901 ggcacaggtt ttgtgatggg tgccatactg gatctgatca gcggctcgac gcttggcgta
    24961 cgcgtattgg cgatgagcat cattgcttac ctggtggcgc tgaaatacca gcttttccgc
    25021 aacctcgcat tatggcagca ggcgctggtc gtcatgttgc tttcgctggt ggtggatatt
    25081 attgttttct gggcagagtt tttagtgatt aacgtctctt tcagaccgga agtgttctgg
    25141 agtagtgtag tcaatggggt gctctggccg tggattttct tgctgatgcg taaagtccgt
    25201 cagcagtttg cagtgcaata aaggtttcta tgacttctct gtatttagct tccggttctc
    25261 cgcgtcgtca ggagttactt gcgcaacttg gcgtgacctt tgaacgtatt gttacgggca
    25321 ttgaggagca gcgtcagccg caggagagcg cgcagcagta tgttgtgcgt ctggcgcgcg
    25381 agaaagcacg ggcaggtgtc gcgcaaacgg cgaaggatct cccggtactg ggtgcggata
    25441 ctatcgttat cctgaacgga gaagtgctgg agaaaccgcg cgacgcagag catgcggcgc
    25501 agatgttgcg caaattatcg ggtcagaccc atcaggtgat gacggcagtg gcgttggccg
    25561 acagccagca cattctcgat tgcctggtgg tcaccgatgt gactttcaga acgttaacag
    25621 acgaagacat cgcgggctat gtcgccagcg gtgaaccgtt agataaagca ggtgcatacg
    25681 gtattcaggg gctgggtggc tgttttgtca ggaagataaa tggcagctat cacgccgtag
    25741 tcggcttacc gctggttgaa acgtatgaat tattaagtaa ttttaacgca ctgcgtgaga
    25801 aaagggataa acatgacggc tgaattgtta gtaaacgtaa cgccttcgga aacgcgagtg
    25861 gcgtatattg atggcggtat tctgcaggaa attcatattg aacgtgaggc gcgacgcgga
    25921 atagtaggca atatctacaa gggtcgtgta agtcgtgtac ttccgggtat gcaggcggct
    25981 tttgtagata ttgggctgga taaagccgcg tttcttcatg catctgacat catgccgcac
    26041 accgaatgtg tggcgggtga agaacaaaag caattcacgg tgcgcgacat ctcggaactg
    26101 gttcgtcagg ggcaagatct gatggtgcag gtggtgaaag atccgcttgg cactaagggg
    26161 gcgcgcctga ccaccgatat cacgctgcct tctcgctatc tggtctttat gccaggggct
    26221 tctcacgttg gtgtttccca acgtattgaa agcgaatcag aacgtgaacg cctgaaaaaa
    26281 gtggtcgcgg agtattgcga cgagcagggc gggtttatca tccgtactgc agcggaaggg
    26341 gttggcgagg ctgaactggc ctccgatgcc gcttatctga aacgcgtctg gaccaaagtt
    26401 atggagcgta aaaagcgccc gcagacccgt tatcagctgt acggcgaact ggcgctggcg
    26461 cagcgtgttc tgcgtgattt cgccgatgcc gaactggacc gcattcgcgt tgactcacgc
    26521 ctgacttacg aagcattact tgagttcacc tcggagtaca ttcccgagat gaccagcaag
    26581 ctggagcatt acaccggacg ccagccgatt ttcgatctct ttgatgtcga aaacgaaatc
    26641 cagcgagcgc tggaacgcaa agtagaactg aaatccggtg gttatctcat tatcgaccag
    26701 accgaagcga tgaccaccgt ggacatcaat accggggcgt ttgtcggtca tcgcaatctg
    26761 gacgacacca ttttcaatac caatattgaa gcgacgcagg ctatcgctcg ccagttacgg
    26821 ttgcgcaatc tgggcgggat tatcattatt gatttcatcg atatgaataa tgaagatcac
    26881 cgccgccgcg tgctgcattc gctggagcaa gcgttgagca aagaccgggt gaaaaccagc
    26941 gttaatggtt tttcggcgct ggggctggtg gagatgacgc gtaaacgcac ccgcgaaagc
    27001 attgagcacg tactgtgtaa cgaatgccca acctgccacg gtcgcggaac ggtgaaaacc
    27061 gtggaaacgg tatgctatga aatcatgcgc gagattgttc gtgtccacca tgcttacgac
    27121 tccgaccgtt tcctggtcta tgcttctccg gcagtagctg aagccttgaa aggcgaagag
    27181 tcacactcgc tggcggaagt ggaaattttc gttggtaaac aggttaaagt acaaattgaa
    27241 ccgctctata accaggagca gtttgacgtc gtaatgatgt aaacagatgc tgggccgcca
    27301 tccggcaaag ggtttttgag tcacatattt agcagacaag gagtgacggg tgaggcgatt
    27361 gccggggatt ttactgctta ctggagccgc gctcgttgtg atcgctgccc tgctggttag
    27421 cggcctgcgt attgctttac cgcatcttga cgcctggcgt ccggaaatcc tcaacaaaat
    27481 agaatccgcg actggcatgc cggttgaagc cagtcagctc tcagccagct ggcagaattt
    27541 tggcccgacg cttgaagcac acgacatccg tgcagaacta aaagatggcg gcgaattttc
    27601 ggttaaacgc gttactctgg cgctggatgt ctggcagagc ctgttacata tgcgctggca
    27661 gtttcgcgac ctcactttct ggcagctgcg ctttcgcacc aacactccta tcaccagcgg
    27721 tggtagcgat gacagtctgg aagccagtca catcagcgat ctgtttcttc gtcaatttga
    27781 ccatttcgat ctccgcgaca gtgaagtcag tttcctgacg ccatccggtc agcgcgccga
    27841 gctggcgatc ccacaactca cctggctgaa cgatccacgt cgacaccgtg cggaaggcct
    27901 ggtaagcctc tccagcctta ccggacagca cggcgtgatg caggtgcgca tggatttgcg
    27961 cgatgatgag gggttgttaa gcaatggtcg cgtctggctc caggcggatg acatcgacct
    28021 gaagccgtgg ctcggtaaat ggatgcagga caatattgcg ctggaaacgg cacagttctc
    28081 ccttgaaggc tggatgacga tcgacaaagg cgatgtaacc ggcggtgacg tctggctgaa
    28141 acagggcggt gccagctggt tgggcgagaa gcaaacgcat acgctgtcgg tggataatct
    28201 gaccgcgcat attacgcgtg aaaatccggg ctggcagttc tctattcccg atacacggat
    28261 cacgatggac ggcaaaccct ggccgagcgg agcattgacg ctggcctgga taccggaaca
    28321 ggacgttggc ggcaaagaca ataaacgcag tgacgaactc cggattcgcg ccagtaatct
    28381 ggagctggca ggcctggagg gcatacgccc gctggccgcg aaactttcac ctgcactggg
    28441 tgatgtttgg cgctccacac aaccgagcgg caagattaac acgctggcgc tggatatccc
    28501 gcttcaggcg gcagacaaga cccgttttca ggcatcgtgg agcgatctgg cctggaagca
    28561 atggaaatta ttaccgggtg cggaacactt ctccgggacg ctttccggca gcgttgaaaa
    28621 tggtttgctt accgcgtcga tgaagcaggc aaagatgcct tacgaaacgg tattccgtgc
    28681 gccactggaa atcgccgacg gccaggcaac tataagctgg ctgaacaatg acaaaggttt
    28741 ccagctggat gggcgtaata ttgacgttaa agccaaagcc gtccatgcgc gcggcggttt
    28801 tcgttacctg caacctgcta acgatgaacc ctggctgggt attctggctg gcatcagtac
    28861 cgatgatggt tcacaagcct ggcgctattt cccggaaaac ttgatgggta aagacctggt
    28921 tgattactta agtggcgcga ttcagggcgg tgaagcggat aacgcgacgc tggtttatgg
    28981 tggcaatccg caactcttcc cctataaaca caacgaaggt cagtttgaag tgctggtgcc
    29041 gctgcgcaac gcgaagtttg ccttccagcc ggactggcct gcattaacta accttgatat
    29101 tgaactggac tttattaacg acggtttatg gatgaaaacc gatggcgtta atctgggcgg
    29161 cgtgcgcgcg agtaatctca ccgcagtgat ccctgactac tcgaaagaaa aactgctgat
    29221 tgacgctgac attaaaggtc cgggtaaagc cgttggccct tactttgatg agacaccgct
    29281 gaaagattct ctgggtgcga ccctgcaaga actccagctc gacggcgatg tgaatgctcg
    29341 cttacatctt gatatcccgc tgaacggcga actggtaacc gcgaaaggtg atgtgacgct
    29401 gcgtaataac agtctgttta tcaaaccact cgacagcacc ctgaaaaatt tgagcggtaa
    29461 attcagcttt atcaatggcg atctgcaaag tgaaccactg acagcaagct ggtttaatca
    29521 gccgttgaac gtggattttt ccaccaaaga aggggcaaaa gcctaccagg tcgcggtgaa
    29581 tctcaacggt aactggcaac cagcgaaaac cggcgttctg cctgcagcgg tgaacgaagc
    29641 attgagtggc agcgtggcgt gggatggtaa agtgggcatt gatctgcctt atcatgctgg
    29701 cgcgacctat aacgtagagc tgaacggcga tctgaagaat gtgagcagtc acttaccttc
    29761 accgttagcc aaacctgcgg gtgaaccact agcggtaaac gttaaggttg atggcaatct
    29821 caacagcttt gaattaaccg gacaggctgg tgcggataac catttcaata gccgctggtt
    29881 gctcggtcaa aagctgacgc tcgatcgtgc tatttgggcg gcagacagta aaacgctccc
    29941 gccgttgccg gaacaaagtg gtgttgaact caatatgccg ccgatgaatg gtgccgagtg
    30001 gctggccctg tttcagaaag gtgcggcgga gagtgtcggt ggtgcagcga gtttcccaca
    30061 acacataacg ttacgtacgc ctatgttgtc gctgggaaat cagcaatgga ataacctgag
    30121 tattgtttcg caaccgacgg caaatggcac ccaggttgag gcgcaagggc gtgaaatcaa
    30181 cgccacgctg gcgatgcgta ataacgcgcc gtggctggcg aatatcaaat atctttatta
    30241 caacccgagc gtggcgaaaa ctcgtggtga ttcaacgccg tcatcacctt tcccgacaac
    30301 ggagcgcatt aacttccgtg gctggccgga cgcacaaata cgatgcgcag agtgctggtt
    30361 ctgggggcaa aaattcggtc gtattgacag tgatctcacc atttctggcg atacattaac
    30421 gctgaccaat ggactgattg ataccggttt ctcgcggctt actgccgatg gtgaatgggt
    30481 taataatccg gggaatgaac gtacctcgct gaaaggaaaa ctgcgcgggc agaaaattga
    30541 tgccgccgca gaattttttg gtgtcacgac gcccatacgg cagtcgtcat ttaatgtgga
    30601 ttacgattta cactggcgta aagcaccgtg gcagccagat gaggcgacgt tgaatggcat
    30661 cattcatact caactgggta aaggcgaaat taccgaaatc aataccggac atgccgggca
    30721 attgctgcgc ttattgagcg tagatgccct gatgcgtaag ctgcgttttg atttcagaga
    30781 cacttttggc gaagggttct attttgactc cattcgcagc accgcgtgga ttaaagacgg
    30841 cgttatgcac accgacgaca cgttggtgga tggcctggag gcggatatcg ccatgaaagg
    30901 gtcggtaaat ctggtacgtc gcgacctgaa tatggaagcg gttgtcgcac cagagatttc
    30961 tgcgacggtg ggcgtggctg cggcttttgc ggttaacccc attgttggcg cggcagtgtt
    31021 tgctgccagt aaagtgctgg ggccgctgtg gagcaaagtc tccattttgc gctatcacat
    31081 ttcgggtccg ctggacgatc cgcaaatcaa cgaagtgttg cgccaaccgc gtaaagaaaa
    31141 agcgcaatga tttgacgagg gcgcgtaatt gccccaatct cataagataa tcgttgccaa
    31201 aggccaacga gccagaacat aaccgtaggc cggatagggc gtttacgccg catccggcag
    31261 ccgttgcctg atgcgacgct gtgcgtctta tcaggcctac aaacggaaca taaccgtagg
    31321 tcggataagg cgtttacgcc gcatccgaca gccgttgcct gatgcgacgc tgtgcgtctt
    31381 atcaggccta caaaccggaa cataatcgta ggccggataa ggcgtttacg ccgcatccgg
    31441 cagccgtaaa aaatcctcta ctgcagtaac taacgagtag caaaaacgat gagtctgaac
    31501 ctggtaagtg aacaattgct agcggcgaac ggcctgaaac atcaggactt gttcgcgatc
    31561 ctcggtcaac tggccgaacg tcgccttgat tatggcgatc tctattttca gtcgagctat
    31621 cacgaatcct gggttttaga agaccgcatt attaaagatg gttcttacaa catcgatcag
    31681 ggcgttggcg tgcgtgcaat cagcggtgaa aaaaccggat ttgcttacgc tgaccaaatc
    31741 agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca ccatcgtccg tgatagtggt
    31801 gatggcaaag tacagacgct gggcgcggta gagcatagcc cgttgtatac ctcggtagat
    31861 ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc tgcgtcgcgt cgataaggtt
    31921 gcccgcgaag cggacaagcg cgtacaggaa gtgactgcca gcctcagtgg tgtctatgaa
    31981 ttaattttgg ttgcggccac cgacggcacg ctagcggcgg atgtccgtcc gctggtgcgt
    32041 ctttccgtga gcgttctggt cgaagaagat ggcaaacgcg aacgcggtgc cagtggcggc
    32101 ggcggtcgtt ttggttatga gttcttcctt gccgatctcg acggcgaagt ccgtgcggat
    32161 gcatgggcaa aagaagcagt gcgtatggcg ctggtcaatc tttctgccgt tgctgcacca
    32221 gcgggcacca tgccggtagt acttggcgca ggttggccgg gcgtgctgtt gcatgaagcg
    32281 gttggtcacg gtctggaagg cgacttcaac cgccgtggca cttcagtatt tagtggacag
    32341 gtcggggagc tggtggcttc agaactgtgt accgtggttg atgacggcgc gatggtcgat
    32401 cgccgtggtt cggtggcgat tgatgacgaa ggtacgccag gccagtacaa cgtgctgatt
    32461 gagaacggca ttctgaaagg ctacatgcag gataaactca acgcgcgttt gatggggatg
    32521 acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc tgcccatgcc gcgtatgacc
    32581 aacacctata tgctgccggg taaatcgacc ccgcaggaaa ttattgaatc cgttgagtac
    32641 ggtatctatg caccgaactt tggtggcggt caggtggata tcacctccgg caaattcgtt
    32701 ttctccactt cagaagcata tctgattgaa aacggtaaag taacgaagcc ggtgaaaggc
    32761 gcaacgttga ttggttccgg tatcgaaacc atgcagcaga tttcgatggt tggcaacgac
    32821 ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag ggcaaagttt gccggttggc
    32881 gtgggccagc caacgttgaa agtcgataac ctgactgttg gcggtactgc gtaataattt
    32941 cagtattttc agaaggataa ttaatctttc tacgtgcacg aacggtcccc tcgctcctct
    33001 ggggttaggg tgaggggaac ccgttggcac aggtttgtac aacgtaacag tacaagatga
    33061 attacttctc tttcccgcgc ccgtgcatct cctgaaacaa tttaccgacc tcaacaaaat
    33121 aatccgtcag cgagttgatc acgacctgta ccttcagcgg cagcttatct ttttcggtat
    33181 ataacgcata aaccgggcgt ggatctgact gataacgcgg cagcaggatc tccagctccc
    33241 cacgattgat ctcgttgatc acccacatca gcggcacgta ggcgatcccg gcacccgccg
    33301 ttagccagcg caccagcgtc atcggatcat tagtcacaaa tctcccttgc gggatcaggc
    33361 gagtcgagat cccttccggt gcaatcagtt caaattcatt gtcgggccgc acgctgtatt
    33421 caagccatga atggctactt aaatcggcgg gtttttccgg tatgccgtat tgtgtgagat
    33481 agcttttcgc ggcgcacacc accattggca tcgcgccaag acggcgggaa aacaggctgg
    33541 aatcctgcaa cgcgccgacg cggatcacca catccagacc gtcggcaatc aggtcggggg
    33601 ctggaattcc ggtaaccaga ttgacgctca aacctgggta ttctttcagc attttggctg
    33661 tcagcccggc gagaacattt tgtgccatag ttgaagaaca gccaatgcgt agcgtcccga
    33721 tgggggtgtt attgaaggca tacagttgct catgaacatc ctgcacttca tgaagcatac
    33781 gacggcagcc ctggtagtaa attctaccgg cttcggtcag gccaatgctg cgtgtgctac
    33841 ggtttaacag ctttacctgc aactcatctt ccagttttga caccgtctga ctgatggacg
    33901 aaacgctcat ctgtagctgt ctggcggcgg cggtaaaaga gccaaattca actactttgg
    33961 caaacaccga catgcgtttt agtcgttcca ttattcactc tgacttaaaa gtgatttaga
    34021 tcacataata tagataacag cataacagtt acgctaatat attaaatatc aatctacagc
    34081 aatgttgctc tcgcccggct ttccatgccc ttctctgcgg cgacagatgc tgaaaataat
    34141 aacgcctgct ctctctatac aaccaaggtc aacatgagtc tgtttcccgt tatcgtggtg
    34201 tttgggctgt ccttcccacc gatatttttt gaattgcttt tatcactggc gattttctgg
    34261 ctggtgcgcc gggtacttgt gccaacaggt atctacgact ttgtctggca tccggcgttg
    34321 ttcaacaccg cgctctattg ctgcttgttt tatttgatat cgcgactgtt cgtttgaggt
    34381 tgaagtgaaa acactaataa gaaaattctc ccgtacggcc atcacggtcg tattagtcat
    34441 tctggccttc atcgcaattt ttaatgcctg ggtctattac accgaatccc cgtggacgcg
    34501 tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg gacgtttctg gactcattac
    34561 ccaggtgaat gttcatgata accagctggt gaaaaaagga caggtactgt tcaccatcga
    34621 ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc gatgttgctt attatcaggt
    34681 actggcacag gagaaacgcc aggaggccgg acgtcgtaac cgtctcggtg tgcaggcgat
    34741 gtctcgcgaa gagatcgacc aggccaacaa cgtactacaa acggttctgc atcagttagc
    34801 gaaagcgcaa gcgacccgcg atctggcaaa actggatctt gaacgcacgg tgatccgcgc
    34861 gccagcggat ggctgggtga ccaacctcaa cgtctatacc ggtgagttta ttactcgtgg
    34921 atcaacggcg gttgcgctgg tgaaacagaa ctctttctat gtactggcct atatggaaga
    34981 aactaagctg gaaggggtgc gccctgggta tcgtgcagag atcacgccgc ttggcagtaa
    35041 caaagtgctg aaagggactg ttgatagtgt tgccgctggg gtcaccaacg ccagcagcac
    35101 gcgtgacgac aaagggatgg cgaccatcga ctctaacctt gaatgggtgc gtttggcgca
    35161 acgtgttccg gttcgtattc gtctcgacaa ccagcaagag aacatctggc ctgcgggcac
    35221 cactgctaca gtggtggtca ctggcaaaca agatcgcgac gaaagccagg attcgttctt
    35281 ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca cgatgggtat tttctccatt
    35341 gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg ccattgtact ggcgctgttt
    35401 gttggctttc acttccagct ggaaacgcca cgctgggcgg tactgacagc ggcgattgtt
    35461 gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt ctggcgctat tcgctatcgt
    35521 ggctttttgc gcatcatcgg cacatttatt ggctgtattg ccggactggt gatcatcatt
    35581 gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct gtatctgggc cggtttttgt
    35641 acctggatat cctcgctggt acgaatagaa aactcgtatg cgtgggggct ggccggttat
    35701 accgcgctga tcattgtgat caccattcag ccggaaccat tgcttacgcc gcagtttgcc
    35761 gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg cgattatggc ggatttgctc
    35821 ttttctccgc gatcgatcaa acaagaagtg gatcgagagc tggaaagttt gctggtcgcg
    35881 caatatcaat taatgcaact ctgtatcaag catggcgatg gtgaagttgt cgataaagcc
    35941 tggggcgacc tggttcgacg caccacggcg ctacaaggca tgcgcagcaa cctgaatatg
    36001 gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag cgatcaatac gctatcgctg
    36061 acgctgatta cccaatcctg cgaaacttat cttattcaga atacgcgccc ggaattgatc
    36121 actgatactt tccgcgaatt ttttgacacg ccggtagaaa ccgcgcagga cgtccacaag
    36181 cagctcaaac gcctgcgaag agttatcgcc tggaccgggg aacgggaaac gcctgtcacc
    36241 atttatagct gggtcgcggc ggcaacgcgt tatcagcttc tcaagcgcgg cgttatcagt
    36301 aacacaaaaa tcaacgccac cgaagaagag atcctgcaag gcgaaccgga agtaaaagta
    36361 gagtcagccg aacgtcatca tgcaatggtt aacttctggc gaaccacact ttcctgcatt
    36421 ctgggcacgc ttttctggct gtggacgggc tggacttccg gtagtggtgc aatggtgatg
    36481 attgcggtag tgacgtcact ggcaatgcgt ttgccgaatc cacgcatggt ggcgatcgac
    36541 tttatctacg ggacgctggc cgcgctgccg ttagggctgc tctacttttt ggtgattatc
    36601 cctaataccc aacagagcat gttgctgctg tgcattagcc tggcagtgct gggattcttc
    36661 ctcggtatag aagtacagaa acggcgactg ggctcgatgg gggcactggc cagcaccata
    36721 aatattatcg tgctggataa cccgatgact ttccatttca gtcagtttct cgacagcgca
    36781 ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta ttttgctggt gcgggataaa
    36841 tcgcgcgaca ggactggacg tgtactgctt aatcagtttg tttctgccgc tgtttccgcg
    36901 atgactacca atgtggcacg tcgtaaagag aaccacctcc cggcacttta tcagcagctg
    36961 tttttgctga tgaataagtt cccaggggat ttgccgaaat ttcgcctggc gctgacgatg
    37021 attatcgcgc accagcgcct gcgtgatgcg ccgatcccga ttaacgagga tttatcggcg
    37081 tttcaccgac aaatgcgccg cacagcagac catgtgatat ctgcccgtag cgatgataaa
    37141 cgtcgtcggt actttggtca gttgctggaa gaactggaaa tctaccagga aaagctacgc
    37201 atctggcaag ctccaccgca ggtgacggaa ccggttcatc ggctggcggg gatgctccat
    37261 aagtatcaac atgcgttgac cgatagttaa gtcaaaaccg acgccaaaag cgtcggtttt
    37321 ttcatggcta tacttagcga tgaacggcag aactcgccgc gaaacgtgac ggtggcaaca
    37381 gatgaatatt tatacctttg attttgatga gattgagagt caggaggatt tttatcgcga
    37441 cttcagccaa gcctttggtc tggcgaaaga taaggtacgc gatctcgact cactatggga
    37501 tgtgttaatg aacgatgtcc tgccgctacc acttgagatt gaatttgttc atctgggaga
    37561 gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt gatgaggcag aggaagagct
    37621 ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa aaaaagcccc cgaaccgggg
    37681 gcaatatcgt cggacaagac gatgagggtt tatttgtaca gttcagccgt agcgtgccag
    37741 gtgtcaccgc tacgagcttc agtaatctgg taggccgttg cgcctttctc ttccgctttt
    37801 ttgttcagca tttcacgcat atccattggc gaagacgcca caccacttac ggatacggtc
    37861 ccgattgctt cacgattttg tgcttgtgca gcatcaatgg agtcggcagc gaatgcaccg
    37921 aaagagagaa cagaaagtac gcttaatgca gcaacagtgg ttttgatttt catgattttt
    37981 acctcgacat aatcttttag ctgggccttt gtttcgtgac ccat
//
LOCUS       DAZGBC010000025        37835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGBC010000025 DAZGBC010000000
VERSION     DAZGBC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.025"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..2357
                     /gene="pdeF"
                     /locus_tag="ACHK1J_001326"
     CDS             114..2357
                     /gene="pdeF"
                     /locus_tag="ACHK1J_001326"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO7910626.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2396..3937)
                     /gene="ppx"
                     /locus_tag="ACHK1J_001327"
     CDS             complement(2396..3937)
                     /gene="ppx"
                     /locus_tag="ACHK1J_001327"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO7910627.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3942..6008)
                     /gene="ppk1"
                     /locus_tag="ACHK1J_001328"
     CDS             complement(3942..6008)
                     /gene="ppk1"
                     /locus_tag="ACHK1J_001328"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO7910628.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6179..6817)
                     /gene="purN"
                     /locus_tag="ACHK1J_001329"
     CDS             complement(6179..6817)
                     /gene="purN"
                     /locus_tag="ACHK1J_001329"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO7910629.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6817..7854)
                     /gene="purM"
                     /locus_tag="ACHK1J_001330"
     CDS             complement(6817..7854)
                     /gene="purM"
                     /locus_tag="ACHK1J_001330"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO7910630.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8179..8805
                     /gene="upp"
                     /locus_tag="ACHK1J_001331"
     CDS             8179..8805
                     /gene="upp"
                     /locus_tag="ACHK1J_001331"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO7910631.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8891..10180
                     /gene="uraA"
                     /locus_tag="ACHK1J_001332"
     CDS             8891..10180
                     /gene="uraA"
                     /locus_tag="ACHK1J_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO7910632.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10275..10976
                     /gene="hda"
                     /locus_tag="ACHK1J_001333"
     CDS             10275..10976
                     /gene="hda"
                     /locus_tag="ACHK1J_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO7910633.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11114..11473)
                     /gene="arsC"
                     /locus_tag="ACHK1J_001334"
     CDS             complement(11114..11473)
                     /gene="arsC"
                     /locus_tag="ACHK1J_001334"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO7910634.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11494..12957)
                     /gene="bepA"
                     /locus_tag="ACHK1J_001335"
     CDS             complement(11494..12957)
                     /gene="bepA"
                     /locus_tag="ACHK1J_001335"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO7910635.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13170..14231
                     /gene="yfgO"
                     /locus_tag="ACHK1J_001336"
     CDS             13170..14231
                     /gene="yfgO"
                     /locus_tag="ACHK1J_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7910636.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVIGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14269..15117)
                     /gene="focB"
                     /locus_tag="ACHK1J_001337"
     CDS             complement(14269..15117)
                     /gene="focB"
                     /locus_tag="ACHK1J_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO7910637.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15139..17151)
                     /gene="hyfR"
                     /locus_tag="ACHK1J_001338"
     CDS             complement(15139..17151)
                     /gene="hyfR"
                     /locus_tag="ACHK1J_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO7910638.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLKKDCDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLIQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17181..17594)
                     /gene="hyfJ"
                     /locus_tag="ACHK1J_001339"
     CDS             complement(17181..17594)
                     /gene="hyfJ"
                     /locus_tag="ACHK1J_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO7910639.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFCCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDTSHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17587..18345)
                     /gene="hyfI"
                     /locus_tag="ACHK1J_001340"
     CDS             complement(17587..18345)
                     /gene="hyfI"
                     /locus_tag="ACHK1J_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO7910640.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDN
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLTGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18342..18887)
                     /gene="hyfH"
                     /locus_tag="ACHK1J_001341"
     CDS             complement(18342..18887)
                     /gene="hyfH"
                     /locus_tag="ACHK1J_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO7910641.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18897..20603)
                     /gene="hyfG"
                     /locus_tag="ACHK1J_001342"
     CDS             complement(18897..20603)
                     /gene="hyfG"
                     /locus_tag="ACHK1J_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO7910642.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTLEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGDSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALRIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAANWPVL
                     RYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSP
                     LK"
     gene            complement(20593..22173)
                     /gene="hyfF"
                     /locus_tag="ACHK1J_001343"
     CDS             complement(20593..22173)
                     /gene="hyfF"
                     /locus_tag="ACHK1J_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO7910643.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRAVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22178..22828)
                     /gene="hyfE"
                     /locus_tag="ACHK1J_001344"
     CDS             complement(22178..22828)
                     /gene="hyfE"
                     /locus_tag="ACHK1J_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO7910644.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22840..24279)
                     /gene="hyfD"
                     /locus_tag="ACHK1J_001345"
     CDS             complement(22840..24279)
                     /gene="hyfD"
                     /locus_tag="ACHK1J_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO7910645.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24296..25243)
                     /gene="hyfC"
                     /locus_tag="ACHK1J_001346"
     CDS             complement(24296..25243)
                     /gene="hyfC"
                     /locus_tag="ACHK1J_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO7910646.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25254..27272)
                     /gene="hyfB"
                     /locus_tag="ACHK1J_001347"
     CDS             complement(25254..27272)
                     /gene="hyfB"
                     /locus_tag="ACHK1J_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO7910647.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGTISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYLVAALVVLLIAIAV"
     gene            complement(27272..27889)
                     /gene="hyfA"
                     /locus_tag="ACHK1J_001348"
     CDS             complement(27272..27889)
                     /gene="hyfA"
                     /locus_tag="ACHK1J_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO7910648.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNENLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPQGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28142..28612)
                     /gene="bcp"
                     /locus_tag="ACHK1J_001349"
     CDS             complement(28142..28612)
                     /gene="bcp"
                     /locus_tag="ACHK1J_001349"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO7910649.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FDVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28612..29184)
                     /gene="gcvR"
                     /locus_tag="ACHK1J_001350"
     CDS             complement(28612..29184)
                     /gene="gcvR"
                     /locus_tag="ACHK1J_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO7910650.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29330..30208
                     /gene="dapA"
                     /locus_tag="ACHK1J_001351"
     CDS             29330..30208
                     /gene="dapA"
                     /locus_tag="ACHK1J_001351"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO7910651.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30225..31259
                     /gene="bamC"
                     /locus_tag="ACHK1J_001352"
     CDS             30225..31259
                     /gene="bamC"
                     /locus_tag="ACHK1J_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO7910652.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31472..32185
                     /gene="purC"
                     /locus_tag="ACHK1J_001353"
     CDS             31472..32185
                     /gene="purC"
                     /locus_tag="ACHK1J_001353"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO7910653.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32353..33216
                     /gene="ypfJ"
                     /locus_tag="ACHK1J_001354"
     CDS             32353..33216
                     /gene="ypfJ"
                     /locus_tag="ACHK1J_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO7910654.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33231..35246
                     /gene="tmcA"
                     /locus_tag="ACHK1J_001355"
     CDS             33231..35246
                     /gene="tmcA"
                     /locus_tag="ACHK1J_001355"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO7910655.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARYGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSNAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35320..36018
                     /gene="ypfH"
                     /locus_tag="ACHK1J_001356"
     CDS             35320..36018
                     /gene="ypfH"
                     /locus_tag="ACHK1J_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7910656.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36099..36299)
                     /gene="ypfN"
                     /locus_tag="ACHK1J_001357"
     CDS             complement(36099..36299)
                     /gene="ypfN"
                     /locus_tag="ACHK1J_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO7910657.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36327..37454)
                     /gene="dapE"
                     /locus_tag="ACHK1J_001358"
     CDS             complement(36327..37454)
                     /gene="dapE"
                     /locus_tag="ACHK1J_001358"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO7910658.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37458..37814)
                     /gene="yffB"
                     /locus_tag="ACHK1J_001359"
     CDS             complement(37458..37814)
                     /gene="yffB"
                     /locus_tag="ACHK1J_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO7910659.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9203 a         9815 c         9961 g         8856 t
ORIGIN      
        1 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
       61 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
      121 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
      181 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttcc tccggtgagg
      241 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
      301 cggcattgcc agggatcgcc ttagggattt ttgtccgcaa atatgcagag cttggttttt
      361 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
      421 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
      481 agcgtatatt ctggcagatt gtgtttcctg caacgctgtt tctgatactt ttccagtttg
      541 ctgcatttgt aggattactg gcgagcagag aaaatctggt tggcgtcatg ccttttaatc
      601 tcgggacctt aatcaattat caggccttac tggtgggcaa tctgattggt gtcccgctgt
      661 gttacttcat cattcgggta gtgcgaaatc cgttttattt acgtagttat tattcgcaat
      721 taaaacagca ggttgatgtc aaagtcacca aaaaagagtt cgcactctgg ctactggcat
      781 taggtgcttt actgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
      841 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcgatg cgctatggtt
      901 ataagctgat ttcactgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
      961 attacattcc catttatccc ggctatacca cgcagctgac cataacctcc tccagttatc
     1021 tggtattctc ttttattgtc aattatatgg ctgtactggc aacccgtcag agaacggtag
     1081 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
     1141 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
     1201 gcatggaaat gctggttaaa aactatggca tcatgctgcg gattcaatac aagcaaaaac
     1261 tttctcactg gctgtcacca ttgctggaac cgggtgaaga tgtttatcag ctttcgggta
     1321 acgatctcgc gctgcgactg aatacagaat cgcaccagga gcgcattacc gcactggata
     1381 gccatctcaa gcaatttcgt ttcttttggg atggcatgcc gatgcaaccg cagattggcg
     1441 tcagttactg ctatgtgcgc tcgccagtga atcatatcta cctgctgctg ggagagctaa
     1501 atacggtcgc cgaactttcc atcgtgacca acgccccgga aaatatgcag cgtcgcgggg
     1561 caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat gatgaatcga ctacagcagg
     1621 cgctggaaca caaccatttt ttcctgatgg cccagccgat taccggtatg cgtggtgatg
     1681 tttaccatga aattcttctg cgcatgaaag gtgagaatga tgaactgatc agccccgata
     1741 gcttcttgcc ggtcgcgcac gaatttggtt tatcgtcgag tatcgacatg tgggtcattg
     1801 agcatacgct gcaatttatg gctgaaaaca gagcgaagat gcccgctcac cgttttgcta
     1861 ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc tgttgaagtc agtcagttgc
     1921 tggctaaata tcagattgaa gcgtggcaac ttatttttga agtcaccgaa agtaatgctc
     1981 tgaccaatgt taagcaggcg caaatcacct tgcagcatct tcaggaatta ggctgccaga
     2041 ttgcgattga tgatttcggc accggctacg ccagctatgc gcggcttaaa aatgtgaatg
     2101 ccgatctgct taaaattgac ggcagtttta tccgcaatat tgtgtcaaat agtctggatt
     2161 atcagatagt ggcatcgatt tgccacctgg cgcgaatgaa gaaaatgcgg gtagtggcag
     2221 agtacgttga aaacgaagag atccgcgagg cagtgctctc tttggggatc gattatatgc
     2281 agggttatct tattggtaag ccgcaaccgt taattgatac gctgaatgaa atcgaaccca
     2341 ttcgcgaaag tgcctgaata atgcgggccg acatttctcg tcggcccgca aagcattaag
     2401 cggcgatttc aggtgtactt tcttcttcaa ttttcaaccg ccagccagcc acgccttccc
     2461 agtattcttg ctccttttcc agatcaagca gtaccaacgc attctgacta aaccagtcat
     2521 gcgggaaacg cagtgtccag tgactgtcat cagtaatcag tgtcaatgtt ggcggtgtgg
     2581 tggttgcctg acgttgattg ttgagtaata cgccaaggcg caatagctga atcagtggca
     2641 ggaactgttt cttcttaaac aaggtaaagc gcggcagatc gtcgagttta atcgctttac
     2701 ggtgatagcg caccagtgtc gccatcatca gctgctgttc ctgattaaaa cccggcaagt
     2761 cgctgttttg tagaatataa gcggagtgac gatgcaaacc gctgtggttg atattcaacc
     2821 cgacctcatg cagcatggcg gcccatcgca gtagcgcctc cagttgcgga tgcgccagct
     2881 tcggttgctg ttcccgccac tgttcgtaca tttgcatagt ggtatccagc acccgtcggg
     2941 cctgttcgct gtcgatgtga tactggttgg cgaggctgct ggcggtgcga ctacgcacat
     3001 cctgatgacg gaaacgtcct tccatttcat acagtacgcc ttcgcgtaac gccccgtcag
     3061 aaaggcgcag ttcacggatg gctaaagcat caaacacacc gcataaaatc gccagtcccg
     3121 gaacgaagac tgttttccgc tcttcggaaa gacctggtaa actcagcgat gcgaaattac
     3181 ggtgacgtaa aacttctttt accagttttt ccagacgttc cggggtaatt atcccgtctt
     3241 tctcgcccat ttccattaat acttcatggg cggcttttat ggtaccggaa gcgcccattg
     3301 ccacgttcca gccctgaata cggaattgcc aggttaaagt ttccagtttt tgtgctgccg
     3361 ccatgcgagc acgctgaaaa ttctctttat tgatgactcc gccagggaaa taaagctggg
     3421 caaagctgac acaccccatc cggcggcttt caacgagaat cggttcgaaa ttttcaccaa
     3481 tcaccagttc tgtagaaccg ccgccaatat caataaccag tttgcgacct ttttccggtt
     3541 gggtatgttc cacgcccata aaaatcagac gggcttcttc attaccggaa ataatttcaa
     3601 tcgggtaggg aatgaccttt tccgcacgtt tcagaaagtc agtggcgttc agcgcctgac
     3661 gcagcgtatg ggtaccaact atacagacgc tggcaggaga aaacccttgt agccgttcgg
     3721 caaacagcga cagacagttc aaaccgcgcg tcattgcctc ttcactcaac atattatctg
     3781 gccccaggcc gtccgccaga tgcacccgct gtttcaggcg gccaataatc tgcatggcac
     3841 catctaccac acgggctatg accatgtgaa aactgtttga accaagatcg actgcagcaa
     3901 actcctgcgg acgaggggat ttatcgtgta ttggcatagg gttattcagg ttgttcgagt
     3961 gatttgatgt agtcataaat cgccaactgc gcccgtactt tgcggcgatt gccgcgggga
     4021 acgtagcgat tactgagttc tttatcgata taacgtgctt tgaccgtatc gctgaacaat
     4081 atgtcgatga tgtccagcac ccgctgcttc aggcgcggat cgagcagcgg cgtcgccact
     4141 tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg aagaaaggta gaccttttta
     4201 tcgccgccat tttcaaaaat ataaacccgg tcatgttcaa ggtaacggtc aacaatactg
     4261 atggcacgaa tgttgtcgct aatgccttcc agattgggga tcagcgaaca cattccgcga
     4321 accagcagat taaccggtac gccggagctg gaggccgcat acagacgatc aaccaggcct
     4381 ttatcgacaa ggttatttag cttcagggtg ataccactgg gcagcccttg ctgcgcgttg
     4441 gcgatctcgc ggtccaccat ttcatacaac aggcggcggg agttttgcgg cgacaccatt
     4501 aaataatcaa atgtcaccgg acggtatggg ttttcaataa agttgaacac ccgccgtact
     4561 tcgttagtga tgcgcgcatc ggcggtcagc aacgaatagt cagtataaag acgcgcggtt
     4621 ttttcgttaa agtttccggt cccaatgtgt gcgtaacgca ccacttcacc gttttcttta
     4681 cgtgaaatca ggaacagttt ggcgtgaatt ttcagccccg gcgcagagaa gataacgtgc
     4741 acgcctgctt cggtcaggcg cttcgcccag tgaatgtttg cttcttcgtc gaaacgcgcc
     4801 tgtaactcaa ccaccacggt gactttctta ccgttgtgtg cggcgtggat catcgagtcg
     4861 atgatgcgtg aatctttcgc cacgcggtaa atgttaattt taatcgccag tacgctcggg
     4921 tcgaacgaag cctgacgcag cagttccagc acatgctcaa aggtgtgata aggataatag
     4981 agcaacacat cgcgttcgcg aatggcatca aaaccattgc ggaactgggc tttatcaaac
     5041 caaatgtggc gtaaacgcgg cagtggtttg ttcaccagat tggctttgcc gacattgggg
     5101 aaattaataa agtctttaaa attatgataa cgaccgccgg ggacgatgga gtcgtagcgg
     5161 gaaatagtca gtttttcgcg taacacttca accagcgcat tgggcatatc gcgctgataa
     5221 acaaaacgca ccggctcagc agttaaacgc tgcttgagac tggaagacat caactccatc
     5281 aggctggctt ccatctcatg cactaaatcg tattcggcat cgcgggtcat cttcattgaa
     5341 taggcattca gcgcgtcata atcaaagaag cctttgaaaa tatcatcaag gcagtaacgc
     5401 agaatgttat ccagaagaat catcggcttg cgtcgacgcg gcgcttctgg cggtaaattc
     5461 acaaagcgcg gcactttatc tgatgggatc tccagcagcg cgtaacggat ggtatcgcca
     5521 cggataattt ccaccgccag ataggtgtaa tcatctttca ggaactgcac taagtcagtg
     5581 tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat actgcttaaa ataatgacgc
     5641 agccagtttt gttgattgac ggagagctgg cgttcattaa tcaggaagat ctggttgcgc
     5701 gccatctcca gcaatagctc gttgtagagg ccgtcgaatt cctgatcggc tttcagcacc
     5761 cgggactgaa ttttgcccag taaatggcgg gaatgagagt tggagccttg ttcttcgcta
     5821 ataatgatgc gtcgcttcag ttcagcgaag cggactttat agaactcatc aaggttattg
     5881 gaatagatcc ccaggaaacg catcctttca atcagcgggt tagatttgtc cgccgcttcc
     5941 tgaagcacgc gttcattgaa cgataaccaa ctgagctctt tttcgatgta tagcttttcc
     6001 tgacccatta ccacttttac tccgttttat tcacgggaca ctgcttggat attatggcga
     6061 gcaatattgg cagatgtcca actatgccag aaaaggggga aaaaaactgc tggagcttgc
     6121 gatttacagg tgaattgaaa aacgccagcg gcagagctgg cgctttaact acggggcatt
     6181 actcgtcggc agcgtagccc tgcggcggca gacgttgacc atccagccac gcggcgtttt
     6241 cgtgcatttt cagacgacca tcggcaaacc agctaatcac cagtggataa atggcgtgtt
     6301 cctgggtttg cacgcgggcg gtgacgtcat cttccgtatc accagcaaat accgggactt
     6361 tcgcctgtaa aataaccggg ccaccgtcca gttcatcggt gacgaaatgc accgatgtac
     6421 cgtgctcttc atcgccattt tccagcgcct gacgatgggt gtgtaatccg ggatatttcg
     6481 gcagcagaga agggtgaatg ttcagcaaac gcccggcata gtgggagaca aacgccgggc
     6541 tgagaatgcg cataaaacca gccagcacga ccacatcggg tgcgtacatg tcgatttcat
     6601 gaatcaactc ccggtcatag gcttcacgac tgtcaaacgc gctggcgatg agcgtatgcg
     6661 ttgcaatacc cgcctggcgg gcgcgttcaa ggccgaacgc gtcggcctta ttgctgaaaa
     6721 ctgcccgtac ggtgccttta attttgttgg ttttacaggc gtcaataatt gcctgtaaat
     6781 tacttccgtt gccggaaata agcaccacaa tattcattat tcgataacca cgcgttgttc
     6841 ggaatcagag gctttgatga taccgatttt ccacgcgttt tcaccgttgg cattgagcag
     6901 ggcgagggct ttgtccactt ccggagcagg cagggcgata atcatcccga cgccgcagtt
     6961 gaaggtgcga tacatttcat ggcgctcaac gttaccagcc gtttgcagcc agttgaacac
     7021 ttccggccac tgccaggagg attcatcaat cactgcctgg gtattatctg gcaatacgcg
     7081 cggaatgttt tcccagaagc cgccgccggt caggtgcgca atggcatgca catcgacctt
     7141 ttcaatcaac tccagcactg acttcacgta aatgcgggtc ggtgccagca gatgatcggc
     7201 taatggctta ccatcaagtt cggtggtttg cggatcacaa ccgctgactt caagaatttt
     7261 gcgcaccagc gaataaccgt tcgagtgcgg accgctggaa ccgagtgcaa tcagcacatc
     7321 gccgtcgctg actttagagc cgtcgatgat ttctgatttt tctaccacgc ccacgcagaa
     7381 acccgcgaca tcgtaatcct caccgtgata catccccggc atttctgccg tttcgccacc
     7441 caccagtgaa cagcccgatt gcagacaacc ttccgcaatg ccgctgatca ccgctgaagc
     7501 ggtatcaaca tccagttttc cggttgcgta atagtcgagg aaaaacagcg gttctgcacc
     7561 ttgcaccacc aggtcattaa cgcacatggc gaccagatca ataccaatgg tgtcgtgacg
     7621 ttttaagtcc attgccagac gcagcttggt acctacgccg tcagtgccgg aaaccagcac
     7681 gggttcacga tatttttgcg gcaatgcaca cagcgcaccg aagccgccca gaccgcccat
     7741 cacttccgga cgacgcgttt tcttcactac gcctttgatt cttccaacca gagcattacc
     7801 cgcgtcaata tcaacaccgg catctttgta gctaagagag gttttatcgg tcactgcttg
     7861 ggtccccacg cgttacttgc ggtagaaaaa taaaattcgg cgcaattcta acagggaaag
     7921 caaacgtttg cgagactgct ttacacaacc tttttgcacg tcttttcccc aggcgcgcgg
     7981 cgaaagaaga cttgtgccag ggcaaaggtt agttttcgga tggaataatc ttctttcata
     8041 accatttgaa tataaaataa ctttatctca aaccgttatc attttgacta aagtcaacga
     8101 aaataatatt gccgccttga agaaaggagg tataatccgt cgattttttt tgtggctgcc
     8161 cctcaaagga gaaagagtat gaagatcgtg gaagtcaaac acccactcgt caaacacaag
     8221 ctgggactga tgcgtgagca agatatcagc accaagcgct ttcgcgaact cgcttccgaa
     8281 gtgggtagcc tgctgactta cgaagcgacc gccgacctcg aaacggaaaa agtaactatc
     8341 gaaggctgga acggcccggt agaaatcgac cagatcaaag gtaagaaaat taccgttgtg
     8401 ccaattctgc gtgcgggtct ggggatgatg gacggtgtgc tggaaaacgt tccgagcgcg
     8461 cgcatcagcg ttgtcggtat gtaccgtaat gaagaaacgc tggagccggt accgtacttc
     8521 cagaaactgg tttctaacat cgatgagcgt atggcgctga tcgttgaccc aatgctggca
     8581 accggtggtt ccgttatcgc gaccatcgac ctgctgaaaa aagcaggctg cagcagcatc
     8641 aaagttctgg tactggtagc tgcgccagaa ggtatcgctg cgctggaaaa agcgcacccg
     8701 gacgtcgaac tgtataccgc atcgattgac cagggactga acgagcacgg atacattatt
     8761 ccgggcctcg gcgatgccgg tgataaaatc tttggtacga aataaagaat aaaaataatt
     8821 aaagccgact ttaagagtcg gctttttttt gagtaaagcg cctataacac ataatacaga
     8881 ggataatact atgacgcgcc gtgctatcgg ggtgagtgaa agaccgccac ttttacagac
     8941 aatcccgctt agtttgcaac atttgttcgc catgtttggt gcaaccgtcc tggtgcccgt
     9001 cttatttcat attaacccgg cgactgtact gttatttaac ggtattggaa cgctgctgta
     9061 tctcttcatc tgtaaaggga aaattccggc ttatcttggt tccagctttg cctttatttc
     9121 accggtattg ttactgttgc cgttagggta tgaagtcgcg ctgggcggct ttattatgtg
     9181 cggcgtgctg ttctgcctgg tttcttttat cgtgaagaaa gcggggaccg gctggctgga
     9241 cgtgctgttt ccacctgcgg caatgggcgc aatcgttgcc gtcatcggtc tggagctggc
     9301 gggcgtagct gccggtatgg cgggtttact cccggctgaa gggcaaacgc cagactccaa
     9361 aaccatcatc atctctatta ccaccctggc ggtcacggtt ttaggttccg tgctgtttcg
     9421 tggtttcctg gcaattatcc cgattttaat tggcgtgctg gtggggtacg cgctctcttt
     9481 cgcaatggga attgtcgata ccacgccgat tattaatgct cactggtttg cgctgccaac
     9541 cctctatacg ccgcgcttcg agtggtttgc cattctgact attctgccag cggcgttagt
     9601 ggttattgcc gaacacgtag ggcacctggt agtaacggct aatatcgtca aaaaagatct
     9661 gctgcgcgat ccaggtctgc accgttcgat gtttgctaac ggcctgtcga ccgtgatctc
     9721 cggcttcttt ggctctacgc caaataccac ttacggagaa aacattggcg tgatggcgat
     9781 cacccgtgtt tacagtacct gggttatcgg cggggcggcg attttcgcta tcctgctttc
     9841 ctgcgtcggt aaactggctg ctgctatcca gatgatccca ttgccggtga tgggtggtgt
     9901 ttcgctgctg ctttacggtg tcatcggtgc ttccggtatt cgtgtgttga tcgaatcgaa
     9961 agtggattac aacaaagcac agaacctgat cctgacttcc gtgattttga tcatcggcgt
    10021 cagtggcgcg aaggtaaaca ttggcgcggc ggaactgaaa ggtatggcgc tggcgaccat
    10081 cgtcggtatc ggcttaagtt tgattttcaa actgattagt gtgctgcgcc ctgaagaagt
    10141 ggtgctggac gcagaagacg cggatataac agacaaataa tgtaccgacc gggcagtgtt
    10201 gctgcccggt tcaaacatca tgggattctg tggtaaactt ctcgcgattt tgtgaaatcc
    10261 tggttgaggt atctctgaac acaccggcac agctctcttt gccactttat cttcctgacg
    10321 acgaaacctt tgcaagtttc tggccggggg ataactcctc tttactggcc gcgctgcaaa
    10381 acgtgctgcg tcaggaacat agcggttaca tctatctctg ggcacgcgaa ggcgcggggc
    10441 gcagccatct gctgcacgcg gcttgcgcgg aattgtcgca gcgtggcgat gcggtgggct
    10501 atgtcccgct ggataaacgc acctggtttg ttccggaagt gctcgacggt atggagcatt
    10561 tgtcgctggt ctgtatcgac aacattgagt gtattgcagg cgatgagttg tgggagatgg
    10621 cgattttcga tctctacaat cgaattctgg aatcgggcaa aacacggttg ttgatcaccg
    10681 gcgatcgtcc accgcggcag ttgaatctgg gattaccgga tctcgcgtcg cgactcgact
    10741 gggggcagat ctacaaattg cagccacttt ctgatgaaga taagttgcag gcgctacagt
    10801 tacgcgcgcg tttgcgtggt tttgaactgc cggaagatgt ggggcgtttc ttgctgaagc
    10861 ggctcgacag agaaatgcgc acgctattta tgacgttgga tcagttggat cgtgcgtcga
    10921 ttaccgcgca acgtaagctg accattccgt ttgtgaaaga aattctgaag ttgtagataa
    10981 ggtgtttatt gtcggatgcg gcgcgaacgc cttatccgaa ctacgcgaag ttaatccata
    11041 gtattcgtag gccggataag acgtggcaag cgtcgcatcc ggcaatgtgc ttcaactagc
    11101 gatggagcac atcttaaccc acgatttcca gtacctgctc cggtggacga ccaattcgcg
    11161 ctttgccatt cgcgaccaca atcgggcgct ccatcagctt cggattatcc accattgcct
    11221 gaatcagcgc ctcttcgcta agcgaactgt cagccaggtt cagttcttta taaagatcct
    11281 ctttctggcg catcaattca cgggcgctat tcatccccag cattttcagc aaatcgcgca
    11341 gcgttgccgc atcggcgggt gtctcaaggt agagcaccac ttctggctcc acgccgtttt
    11401 ctttcagcag gttcagcgtt tcccggctct ttgaacagcg tgggttatgg tagattttta
    11461 cctgtttggt cattgttctt cctttaatgc gaattacatc ttggtataag gcttaaagcg
    11521 ttcctgcagc tggcgcaact ggtcgatgcg cgcatcgtaa cgcgcttgtt gcaggctgcc
    11581 taatttcacc tgcgaactgg cgctactcaa cagcgaaatg gcctgatcga gtcgtccggc
    11641 gagcgcataa ccttctgctc gcgcagccag ctcctgatcg cggttattta gcgcggcttc
    11701 cgcctgtgcc agcaaatccc agccgttgct gtcatcttta ttattaaagg tgtagcgatt
    11761 cagaatattg gccgcttctt gtggttgacc gccttgcaga taagcgttcg ccaggttgag
    11821 ctgcaacaca ggattggtgc gtaaatcgcg ggcatttttc agacgattga tcgcctcatt
    11881 ggctttgttt tgcccaagat cgatatcagt agccagatcg agataccatg cgttgccagg
    11941 ttctgccgcc agtaacggtt gcagcgtttt acgcgcctcg tcgtatttat tggcttccat
    12001 tgcctgtaaa gcacgaccat attgtgctgc tcgttgctga cgaacgtttc ctttcgccca
    12061 ttcatccagc aaatcactgg tgagctggtt acgtccggaa ttatacatcc ccagtgtgcg
    12121 cgctttcgcc agatagaaat cttccgacga ctgcaccacc atcgggcgca tctgattagc
    12181 acggttgcgg gcatctgcca gacgactttc cggcaacggg tgagtcaata aaatttccgg
    12241 cggacgcgag gagtaacgcg cctgatcgag taatttttcg aggaaggtcg gcatcgcctg
    12301 cggatcgaat cctgagcgtt gcagcacctg aataccaatg cggtccgctt cctgttcatt
    12361 ttgctgggtg aaactgatca tcccctgacg cgttcccgcc agtgtaccgg tcagcgccgc
    12421 catccccgcc tgcggactgg ccatcgccag taaaatagaa cctaacgcgc cgacccaggt
    12481 cagcggcgcg ctgcgctgct gatcttccat cgctcgcgcc aggtgacgtt gggtgacgtg
    12541 ggagatttcg tgcgccataa ctgaagccag ttgactttcg ttatcggaat aacggaacag
    12601 ggcagagtgc agcaccacgt tgccgccaaa gaaagcaaag gcgttaattt cgtcgttgtt
    12661 gatcagaaaa aaatgaaacg gtgtcttaac cgaattggca tgcgaaacca gacgcatccc
    12721 cagcgaatta atatattgcg ttaacagcgg gtcattaatt aacggcgcgc tgccgcgtag
    12781 ctggcggaca taatagtcgc ccatctgcat ttcctgacca atggaaagcg tgcttcctgc
    12841 ggaggttccc atatccggca aggtgtctgc gctgtcggca aacgccgggg ctacctgacc
    12901 aatggtcata gcagcaatga gggttgcaac caggtttttt ttcaactgcc tgaacataac
    12961 ctctatcctg tatttctgaa gatagtcatt tgaccgatac tgtgaaatat cgttcccacc
    13021 gcgccgcctt tgagtgtaac ggcgaagatt agcccactca aatgactttt cagtttcctg
    13081 aatccgtcat aaaaagcgaa gtcttttttg tgacatttcg atacaattgc ggatcgcaat
    13141 cccgctcttg aggttcaggg aaggtcttta tgctcgaaat gttgatgcaa tggtatcgcc
    13201 gccgttttag cgacccggaa gcgattgcct tgctggttat tttagttgcc ggatttggca
    13261 ttatcttttt ctttagtggc ctgcttgctc cgttgctggt ggctattgtg ctggcctatt
    13321 tgctggaatg gccaaccgtg cgcctgcaat ctattggctg ctcccgccgc tgggcgacgt
    13381 cgattgtatt ggtggttttt gtcggtatat tgctactgat ggcgttcgtg gtactgccta
    13441 tcgcctggca acagggcatc tacttaatcc gcgatatgcc ggggatgctc aataagcttt
    13501 ctgactttgc cgccacgttg ccgcgccgct atccggcgtt aatggatgcg ggcattattg
    13561 atgcaatggc cgaaaatatg cgcagtcgga tgctgaccat gggcgattcg gtggtgaaaa
    13621 tttccctcgc ctcgctggtc ggtttgctga ccatagccgt ctatctggtg ctggtgccat
    13681 tgatggtctt cttcctgctg aaagacaaag agcagatgct gaacgccgtt cgtcgggtgc
    13741 tgccgcgtaa ccgtgggctg gcaggacagg tgtggaagga gatgaatcaa caaatcacca
    13801 actatatccg cggcaaagtg ctggagatga tcgtggtggg gatcgccacc tggctggggt
    13861 tcttgctctt cgggctgaac tattcgctgc tgctggcagt gctggtcggc ttctcggttc
    13921 ttattccgta cattggcgca tttgtggtga ccattccggt gataggcgtg gcgctattcc
    13981 agtttggtgc tggcacggaa ttctggagct gcttcgcggt gtatctgatt attcaggcgc
    14041 ttgacggcaa cctgttagta ccggtgttgt tctccgaagc ggttaacctg catccgctgg
    14101 tgattatttt atcggtggtg atcttcggtg gtttgtgggg attctggggc gtattcttcg
    14161 ccattccatt ggcgacgctg atcaaagccg tgatccacgc ctggcccgat gggcaaatag
    14221 cgcaagaata atcttcagcc cgacgaactg tttcgccggg ctgatttttc aggattcctg
    14281 acgtaaataa atagcccgat aacacatact caccagcact gcaccgccga taatattacc
    14341 cagcatcacc gggagcagat tggcggtaat aaaatggctt accgtcagtg ccggaaaatt
    14401 gtctgcgcta ctgtgcgcca gttgccagaa ggaggtaggg gcgaaatggc gaatggcaat
    14461 ggcgaacgga atcacaaaca aattggcgat acagtgctca aagccactgg cgacaaacag
    14521 ggtgatgggc aaaatcatgg cgacgatttt gtcgcataac gaacgcccgc aataactcat
    14581 ccacagcgcc aggcaaacca tcagattgca cataatgccg aggctgacag attcagtaaa
    14641 tgtatgatgc attttgccct cggcgcagtg taaaaccgcg actccccaca tcgcgttttc
    14701 actcatcacc agcccggaaa accagattaa caaactgaaa caggcaatac ctgccagatt
    14761 accgcaggcc accagaagtg cgttaatcag ccaagttcgc caactaataa cgccccgact
    14821 ttttgccatc accgtcatta ccgacgaggt gaacaggctg gtgccgcaaa ccgccagcaa
    14881 aataaagccg agtgtaaagc aaaggccgcc caccagatgg gttaatgcct gtgaagacgg
    14941 ggcatcggcg ataacggaaa ggtaaaaagt aaatccgatc gccataaaca cgccagcgga
    15001 catcgccatt aaaaagaccg acactttact gcgggcaatt ttatgggcgg caatccgttc
    15061 agcgattgcc gcttttctgg cgctcaactg caagtcgaaa gagagtttgt ttctcatgca
    15121 tactcctgca aaagcagatt acaacacctc gcgaaccgag atccccagac gctgcattcg
    15181 tgacagcagc gtggtgcgct tcatccctaa tcgtgtcgcc gcgccacggg gcccggcaac
    15241 aatgccattg gtttctcgca atacctgaac aatgcgctga cgttcttctt cgtcattctc
    15301 tggcgtcgtc gggtgcagca actgcgccat tgaactgcga agcgctgaat cttcatttag
    15361 cgtcggcagt aaacggcttt gtcggacatt aagatgtaaa ttcagactgt taccacgagt
    15421 caacagtacc gcccgctcaa tcacgttttc cagctcgcgc acgttgcccg gccaatccca
    15481 cgacatcaac tggcgtagcg cctcggtcgg gatggcgtca attgcgcgat tcatatggcg
    15541 cgccattttt tgcgtgaaat gttttgctaa aagagggata tcttccggac ggtcgcgtag
    15601 cggcggcaat tccagtggga agacattcag gcgataaaag agatcgctgc gaaactggcg
    15661 atcttcaacc atttgccaca aatcacggtt ggtggcggca atgactctga cattcaccgg
    15721 gatcgttcta ctcccgccga gacgctcaat ctcccgctcc tgcaatacgc gcagcagttt
    15781 aggctgaagt tctaacggca gatcgccaat ttcatcgaga aacaacgtgc cgccatcggc
    15841 aatttcaaaa cgaccacgat gggtattaat cgcaccagta aacgcccctt tgtcatgacc
    15901 gaataactca ctttccagaa gactggcggg gatggcagcg cagttgattt tgaccagcgg
    15961 cttgtcgcgt cgcgggctaa gttgatggat cgctctggcg attacctctt tgccagttcc
    16021 ggtttcaccg caaatcaaca ccgtactgtc gctcttcgcc acaatatcta cctgctggag
    16081 caggtcttcc attgcctggc tttgatagat aatgtcaccg acgcccagat tcgaaaggag
    16141 ctgctcgcta agctggtggt tttcttgctg caaactttcc tgcaaatcgg tcatgctacg
    16201 ccaggcatcg gcattgccaa cggcaatagc gatgcgatcg gctatgtgtt gtagtagctg
    16261 gcagttttcc tcactaaaga gagtggaaga ggtatgcgcc agcaacaatg cacccggtgt
    16321 atggttgcca aaggtaagcg gtataaggag cgcagattcg cagccgttag atgcaagtaa
    16381 gtgtaataac gggtcgcgtt gccagagaaa cagatcatct gcacggtgcg tcaaggtcgg
    16441 ttgtcgggtt tgtagcgttt gtatcagcaa tacactgtcg ccaggcatat tgcgtggcag
    16501 acacgcacaa tgtgaggcag aaagatcgct gcaccacagg ctgaacttct cgttctttcg
    16561 atgatcgccc agtaccatac tgactgaagc cagaccgaaa aaatgatgga tctcacgggc
    16621 gacgtcagcg atcagatcgt cgagatcaag atgtgacagc actgcattag taatatcaac
    16681 cagtacccga tgatgatcgc aatctttctt cagcaaacgc agcgccacct gctgttcttc
    16741 atcattgagc catgattgca gaaccggggt gataatgcgt gccagccagt taaacagtgt
    16801 gcaatcctgg ggagtaaaac gactgccgtg ggtgcgaata aagcgtagct ccgccattcc
    16861 actactgccg agaaggacgg cgtgacgcga ataatcgacc atctcgccct gatactctac
    16921 tgttgccgtc acatcgctgc catcatcatg gtagcggtaa tagaagttgc ttttgagaac
    16981 atgcagttca ataagttgta ccgggctgaa cggcggcggc agcgggatga aggcgcgtaa
    17041 taaagacgcc ttgccgtgtt tctgcgctaa ccgctccgcg agcattccgt ttaccgcttc
    17101 aattgttatt ccttgcggcg gggcaaacat cgcctcgtct gacatagcca tcaacagtcc
    17161 ttattaatga gaaatccttc tcacgcaatt tttctggcca tcagatagat ggccggttcc
    17221 tgcacaattt catccaacag atgaatgaat tgaatgctac gcgcctgcca cacagacgtc
    17281 gcgtcatccg ccagtggcgc gaatgcccgg gtcaacaggg cggtgtggct ggtgtcaata
    17341 acaatctcac caaaagtcat cacccccagc atcttacgtc gggcttgctc ctcttcgacc
    17401 agtgcaagcc aatcttcgta ttccgtcagt gggcagcaga aggcgacatt cagacaatca
    17461 atcacgccaa cgtgatgacc gatagccagt gagtaataca ttacctgaga gctgtgttcc
    17521 ggcatctcgt cgctactggc gacaaacttt tttcgcagcg tccagaaaac aatttccccg
    17581 cactcctcag ccatgtaacc cccggagaac gcgaaagagt tgctgaacga tactattgag
    17641 acgcggatcg tcggcatcgc gcaaccaggt gttaacccga tttcctgtag gatcatcgct
    17701 taaatgacgc aggagccgat cgcaaatttc tcgcccctga cgatagcccg tcagccgccg
    17761 cgcttctcgc tcaatggcga tacgctgtga tggcgggatc tgcggccaca acggttgcat
    17821 agtcaccccg gtgggatcgc gataatccac agcgtgaatc ttctgttgca gcaaaccgag
    17881 cgccacggcg aaaccgtgaa tggtggcggc cggtgttggc gggcagccgg ggatccaaac
    17941 atcaatgggg acaatggtgt cgctgccgcc ccagacgctg tagagatcgt ggaaaatacc
    18001 gccgccgaca ccgcacgcgc cgtaggaaac acaaatttta ttatcggggg cagactcata
    18061 cgcccgaagt gcaggcatac gcatcgcccg ggtgactgcg ccagtaaata acaaaatatc
    18121 ggcgtgacgc ggtgatgaaa caaccttaat gccaaaacgt tctgcgtcga atacgggtgt
    18181 gatggcagca aaaatttcga tttcacaggc gttgcagccg ccgcaatcga cgcgataaac
    18241 gtaagccgag cgacggatat cttgtaacaa atgctgcttc atcttttgcg tttgctcgtc
    18301 cagcgtgatg ggctggctga catgttgcgt aagcactgga ctcatagctg ctccttagcc
    18361 accagcggta catctgtatc gtcgttgatc agcgtcgcgc gttgtttgca ctccgggcag
    18421 acgctcgctt gcgcccgcaa catttcgcgg ttttgtggtg cattttgttg ctgtgccaac
    18481 aattcagcag ccagtgcgac ggttttttgc ggggcaaacg ggcgttcgca acggctgcaa
    18541 cgttgtagat ggaacgtcgc gcgggtatag agatcggctt tattggtgac ggtcagttca
    18601 aagttattgg taagctggat ggctctggtc gggcacactt cttcacaacg tccgcagtaa
    18661 atacaacgcc ccagatagag ctgccaggtg cgcgaatttt gctggtcgtc ggtctggata
    18721 gtcagcgcat ttgccggaca agcacaggcg caggcaccgc aggcaataca ttggctgggc
    18781 atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa gggatatttc
    18841 accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt ctgtccttac
    18901 ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata cggcacggtt
    18961 tttgactggc gcttgcgcac atcaaccagc gtcacacggt cggtacagga gtagcaggga
    19021 tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgtag catgtaacgc
    19081 agcaccggcc agttggcggc acgacagcgc cagcggaaca atttttggtt atcaccgagc
    19141 atgctccagt gcacgtcttc accgcgtggc gcttcaacaa agccgagcgc gaatgcgtga
    19201 ggtttatagc taaagccttc ggtcagcagc ggggtatccg gcatgttgtc gagggcaaat
    19261 tccagcattg ccagcgaatc aaacgtctct ttgacacgga ccatcacgcg ggagaaaaca
    19321 tcgccgccgg tgaaggtaaa cagtgttttt ggaatattgc cgtagtcggc gtaggggtga
    19381 tcaaagcgca aatcacgggc aaaaccactg ccgcggatca gcggccctac agggctataa
    19441 tcacgggcga tttgtcggtc gagaatgcca atgccctgag tgcgttgttc catattcggc
    19501 gtagcaagca gcatctctac cagctccgac acgtcggcgc gcatctcgcg caccagtttc
    19561 agcgtttgca gacgttgctc tttgagaata tcgcggcgaa caccaccaat cagattcaga
    19621 ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg tcatcgactt ttcccgcacg
    19681 cgaaaaaatt gcataaagcc ggtatcaaaa ccaacaaaat ggcaggagag gccaaggttg
    19741 agcaaatgac tgtgtagccg ttcgacttcc agcagaatcg agcgaatggt atgcgctcgt
    19801 tgcggcacct caatcctcag tgcattttca accgagttgg tataggccac actgtgggca
    19861 aaaccgcaaa tcccacacac gcggtccgat aagaaggtca cttcgttgta gcccatccgc
    19921 gtttctgcca gtttctccat gccgcgatgg acataaaaca gacggtaatc agcatcgaca
    19981 atttgctcgc catccacaaa caagcggaag tgacccggtt catcggaggt gatatgcagc
    20041 gggccaatag ggatcacccg cgcgtcgctg tcgccctcgt tgataaacgg atacgtttcg
    20101 gtatcagtcg tcggttcagg gcgcagtcga taatccatcg cgtctttgcg cagcggatgc
    20161 atatcttccg gccagtcatc gggcaacacc aggcgacgct gatccggcag gccaaccgga
    20221 atcagcccgt acatatcgcg aatttctcgc tcgccccaga ccgcggcagg gacgcgcggt
    20281 gtgactgacg gaaactcccg actgtcggca tcgaccagtg ccttcaccac gatccagcat
    20341 ttttcggccc cttccataga aagggcataa taaacggcgt aatgaccgtt aagtgtccgc
    20401 tcgtcgttgc caaacaggac cggaagccag ccatcatgtt gataataaag atactgtaca
    20461 acgtcaggca gcagattgat tttcaccgta atggtgacct gttcaagcgt ttgtcgctct
    20521 tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc ttcgccacga
    20581 tttgacgatg aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc ctgaaggcaa
    20641 aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc gtccctgaga ggactatagt
    20701 ggaagcgcct gccaggatcc tgatgacagg ttgtggaata tgcgttccca tcgccagcat
    20761 catgaccagc agaatcacca ttggcgaggt ggtcaaccag ccaagttcac cccggttaac
    20821 ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc cgtaccaggc ccgccagcac
    20881 cagcgttaac agcaataaca gcaggacgat aatcagcaga tgattacgtg ccagtccggc
    20941 ggtaacggtc ataaattcgc taagaaaaat gttgaagggc ggcatccctg ccagcgccag
    21001 cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt ttgagcatcc cacagacgac
    21061 gttgagatcg cgcgtgccgt acttgagcag tacattgccg gaaccgcaga acagcagcgt
    21121 ttttgccaga ctgtggttta aggtgtgcag cagcgcggca aaaattccca gcggcccgcc
    21181 aatgcctagc gccaccgcga ccagtcccat gttctccacg ctggagtacg ccagcagacg
    21241 cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca acatgccgaa
    21301 gatgagcaac aaccggttgg ggaaatcgct gccgatggcc tggcaaataa tgatgtaata
    21361 gcgaatcagc accaacagcg cgcagttcag caatacggcg gagagtaggg cgctgaccgg
    21421 actcggcgct tcactgtgag catccggcag ccaggcgtgc atgggaaata gcccggtttt
    21481 ggtaccaaag ccaattagca caaacacaaa ggccaacagc attaatgttg gatcaagcaa
    21541 ggacgattgc ttaagaacct cgctccagaa tatcgccatt tctgcctgcg gcataacgct
    21601 ggcggcgttg gcgtatacca gcacggtacc gaacagacca aaagcgacac caacagtaca
    21661 aataatgatg tacttccatg cagcttccag cgatgaacgc tgaccgtaaa tgcctaccag
    21721 aaacgccgag cttaaggtgg tggcttcgat cgccgcccac atcacaatca ggttattgct
    21781 ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt aatcgcacag
    21841 cgtaacgggt gaaagctcgc cgtgctccac ttcatgacgc atgtagccaa tcgagtaaac
    21901 accggtaaga aagccaatca caccaagaat ggcgaggaac aaaccgccca gaccatcaat
    21961 atgaagccac agtcccgcag cgaatatttc tcccgcatca gcggcggttt ggaccaccca
    22021 gagtgccaga atcagcagta gtgtgatccc taagctatgt aatacggtca ccgcgcgagt
    22081 cgcagagtgc ccccgtttcc ggcaggcaaa acagagcagc gaaaaaagca gcggcgtgag
    22141 caggagtaaa gcgaacatca cagaataact catctcatta tcccttcagc gcggtcaagt
    22201 tgttcacgtc cagcgtgccg tgggtacgcc atatttttct tgccagtaac accatcacaa
    22261 tgacggcgaa gatggcgtcg gtagcgatac ctatttccac cagttccggt gcacgccagg
    22321 caagaagcgc cagcaccaga tgggagccgt tttccatcag gcagtaacca aaaatttgcc
    22381 gcaggatatt gcgctggctg acaatgcaca gcaggccaag cagaaaatga cctaacgcta
    22441 ccgccagcgc cggtttcagc ccggtagcca tcggtagctt cacgggctga acgacaaatg
    22501 cgcaaagcag gacaattaac gccgcgagca gtgccatcat tgccggaccg aataacgctt
    22561 tttccgggat gttctgggga atatttcgtg cagcgtaagt catgattaac ggtaccagca
    22621 gcactttggt gataaaggcg ctggcggacc agatcagcag ttgctctgcg gcgaacaggc
    22681 acgagagagt ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa aatccgcatg
    22741 acaggcgata gcttttgacg ctaatcacaa acagcgatgt cagcatcatc agtcccgcca
    22801 gattatttac gatcatagaa ccggtcattc atcatctcct tactgcaacc aggtcgcggc
    22861 gattacgctg gaaatcagcg acatcacaat cagtacaatc aacaccaggc gcattgatcc
    22921 tggcagcggt gcggcatcgg cgacggcctc gctcggtttg ccaggcacga cgcgaccaaa
    22981 ccagcgaata aaccaagcga aactggcgac cgattcaatc atcagcagaa tcatggcggg
    23041 cagcaggatc cagtactcca ctgacaacgc aaaaccggcg gcaaacagcg ggaatttact
    23101 gaagaagccg ttgaacggcg gcacgccggt aatcgccagc gctgccacgc agaaacccac
    23161 gcctggcaac ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc gcgtgccgca
    23221 gctgtaactc agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg cgtggttgac
    23281 gatgtaggcg atgctaccct ccagcgccag ccgcgagccg aagatggaga gcgacaagcc
    23341 gaagaacatc cagccaagtt gagtgatggt cgaccaggcc agcaaccgct tcatatcctg
    23401 ctgtggcaaa tacatcagaa atccataaag aatggtgacc agggccatga ccatgccaac
    23461 accgccaatc acatgcggga tattgccgcc gtcgataatg gcgcgggcaa aaatgtaaac
    23521 gcccactttc accatcgatg cggcgtggag ataggcgctg atcggtgttg gcgcttccat
    23581 tgcgtccggt agccacgctt gcatcggtag ctgggccgat ttcccccacg cggcaaacag
    23641 gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac tcgctcatcg cgctaagcgc
    23701 aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca acgagccgat
    23761 atgggtgata agcaacgctt ttagtgctga acgctgcgct ttatcgctct ggtaataact
    23821 gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac aacaactgac cgagcagcgt
    23881 cgaggagagt accagtcccg ccatcgcgcc gataaacaac agcaaaaatg cgtaataacg
    23941 attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc agataacccg tggagtagat
    24001 cgtgaccagc aaaccgagaa acaccaccac aaacagaatc agcgtactca cgcgatcaat
    24061 gaccaggcca aacaatgcca catcccccac gttgaccaac gtcaacgtga cggcaacttt
    24121 atcggcctga taaaacgcgg agatcagtga caacatgcac agcgtggtca gcgcggcgaa
    24181 caaaaccccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat
    24241 aaaaggcagc aataacgtcg tcagagcaag attttccata ttccgtcagt gctccttaca
    24301 gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc caggtcacat
    24361 ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgatagag gccagaacaa
    24421 aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag gcgagagaaa
    24481 gctcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc atcacgacct
    24541 gtttcagccc cagcccccat ttcgctagcg ccagcccggc accggaatat tcggtcagcg
    24601 ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt cccatctcaa
    24661 tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc gagttccagc
    24721 ccgttgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc agcgccaata
    24781 ccagcagtga gagaatgagc atgggttcga ccagaatgcc gagcgtcaac tcgcgactgg
    24841 caccgactcc cgcaaacggg cttccggtat ccagcccgga aagagcaaag aaaaaacgaa
    24901 acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg
    24961 taataaatag tggtagggcc atcgccagca ccagcatgct gctgattaat acccacggca
    25021 tcaggcggaa catcagacct gaagatgtcg gcgcaacttc ctggcgttta aacagtttgt
    25081 ggatatcgcg ataatcctgc cagatccccg gtccgcggcg ggagtgcatt cgcgcgcgta
    25141 tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg gcctgtgcta
    25201 acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt tccttagacg
    25261 gcaatagcga ttagcagcac aaccagtgcg gcgaccagat acaggcaata gagacgaaag
    25321 tcgccgcttt gcagatgctg tatttctttg gccagccgtt gggtggcgct cacgatgggg
    25381 cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt gacgtgcgca
    25441 agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa tagcgcagaa
    25501 aagaccacac gcagcggctg catcacgcca ttgcctgacg gggccatcgc attttcccag
    25561 ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac
    25621 cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa cagtaatgag
    25681 ggggtgacct gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc gacagtgacg
    25741 ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag ccaactggca
    25801 ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat catcgtattt
    25861 ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa agtaataccg
    25921 tacattttta cgaagcacat tactgccagc ccaccagtga ctgccagcat taccatagca
    25981 ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa cagcgactgc
    26041 caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat cgcgaggcaa
    26101 ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc cattttttcc
    26161 atgtcgtggg tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag tagccctttg
    26221 aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac ggtgagcagc
    26281 gggtcgtgca gtgacagacc gaccatcgcc acaccgactg ccagcaaaat aatgccgacg
    26341 ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag cgcatatagc
    26401 acgcccagga gcgcggagat tgtgccgatc gccatcacca gaatgcccca ccacagcggc
    26461 aaacccgttt gcgccagcag atccatcgcc actttcagga tgccgaaaat accgattttg
    26521 accattacgc cagacatcaa cgccgaagcg tgcgatggtg ctgcagggtg agcgcgcggc
    26581 aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa ggccagcagg
    26641 aacaccgccg acgccagccc cggagaaagt gaaagcgtgc ggaaactggc aaaatcgagg
    26701 ctgccgcttt cgcgtcccat cagcaagaag gcgatcataa tcagcaccga tccggcgtgg
    26761 gcgataaaaa agtagagcat gccagcgttg atcgacgttt tatcctgcct ggcaatgacc
    26821 agaaaccagg aagacagtga catcatttca aacagcacga tgaaccaaaa agcgttgtcc
    26881 atcaccagca gggcaaccat cgatgcgatg aaaatattca taaagaagcc catcgccgcc
    26941 gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat agagcgaaca aaccaccacc
    27001 agcaaggaga taaccagcac cataaatgcc gagaggctat ccattcgcag ggttaaatcg
    27061 gcaaagtcaa aaggggtggc aaaacgggcg actaaagtta cgcctgcatg taattgcgta
    27121 attccgctga taatgccaat cacgccgccc accagcgatg tgatgccgga aagcttaata
    27181 gccagtctgt ccagaccgag taaaaacagc gaagccagac tggcaaacag atagaggatc
    27241 agcgaccagg ttaataattg cagggcatcc attagcgttg ctcctgagtg agggaaaggg
    27301 gatcctcccc gccattggca aaccagcttg cggcaaggcg ctgtttttct ttcatctgac
    27361 gttgcaggct atcgtcggtg atcagccgta acgcctgatt cgggcaagcg cgaacgcagg
    27421 ccggaccttg tggcaagaaa tcacacaggt cgcatttcac cgcgacggtc tggacacccg
    27481 gttcccagcg cagcaaagca tgttgggttg gcgcgttttc ttcgccgtct ttgagtgagc
    27541 cttcagcctg aaaaacatat tgcgcagggg cattcaccgg acggcttcct gaagcgctga
    27601 ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc aatacagagg ttttcgttga
    27661 gttggatcgc atcatccctc tgggagatgg cattgaccgg gcagacctgc aggcacgggg
    27721 cttcctcaca gtgatgacac acgacagggg cagtgagtgt tgacgtcttc gccagggcca
    27781 ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg
    27841 tattacatcc tgtacaccac agtggttcgg ccaccacaaa gcggttcatg tcggctcctt
    27901 gaaaatgata tgaaacattg catttcatat tgcataaagg atgccagtct gattaattta
    27961 atatattcaa taagttatga attaactgga ttctgaacgg tacaaaaatg atggtcatta
    28021 acgttcagca aaaaatggat tcgacacccg tgtcgcaggg gaacgcgaga atgtgatctt
    28081 gatcacgaga agtgaggtat gctgaccgca agcgcagcca gcacggaatg gagcaaagta
    28141 atcaggcgtg ttctttcaac cagttcagca caacgtcgtg gtgattgctg gttttgaaat
    28201 cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag gaagctgatg cgatgaatac
    28261 catcgtaggt tttgcccatg aaggactttt ccccccagac gtcgaattgc tcgcacacct
    28321 ggtggtcctc atcagacagg agcgtaaagt taagcagctc tttttccgca aaacgggaga
    28381 gtttttcggg tttatcggtg ctgataccca gcacatcaac gcccgctttt ttcaactcat
    28441 ccatgttatc gcgtaagccg caggcctgta cggtacagcc gggggtcatg gctttcgggt
    28501 agaaataaac cagaacacgc tgtccctgga agtcggtcaa attaacttgt tctccgtctt
    28561 gatccggcaa gctaaatttc ggtgcgatat caccggcttt cagtggattc attacttaac
    28621 tccatcctgt tcatcatgtt gggaataatt gacgacgtta atactgcctt gtgcattgag
    28681 ttctgtacat agggctttga acgcttgctc aatatttgct gcgtccgcag atgcggggct
    28741 gtgggcagta atctgaatat gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg
    28801 cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat
    28861 taaatgcggg gagtctgcca catcgacctg aacccagaca gatgctggca ttggcggacg
    28921 cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc agttcggcac ctttcaacgg
    28981 taacgttgat tcaatcagag taatggcatt ccatgaaccg gaaagcagca tgataaacgt
    29041 gaactcttct cccagcatcg ccaggcgact gtcttcaata ttacagccgc aactactgac
    29101 atgacgggtg atggtgttca caattccagg gcgatcggca cccaacgcag tgatcaccag
    29161 ataatgttgc gatgacagtg tcaaactggt tattccttta aggggtgagt tgttcttaag
    29221 gaaagcataa aaaaaacatg catacaacaa tcagaacggt tctgtctgct tgcttttaat
    29281 gccataccaa acgtaccatt gagacacttg tttgcacaga ggatggccca tgttcacggg
    29341 aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt
    29401 gaaaaaactg attgattatc atgtcgccag cggtacttcg gcgatcgttt ctgttggcac
    29461 cactggcgag tccgctacct taaatcatga cgaacatgct gatgtggtga tgatgacgct
    29521 ggagctggct gacgggcgca ttccggtgat tgccgggacc ggtgctaacg ctactgcgga
    29581 agccattagc ctgacgcagc gcttcaatga cagtggtatc gtcggctgcc tgacggtaac
    29641 cccttactac aatcgtccgt cgcaagaagg tttgtatcag catttcaaag ccatcgctga
    29701 gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct gcgatctgct
    29761 cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca aagaggcaac
    29821 agggaactta acgcgtgtaa accagatcaa agagctggtt tcagatgatt ttgttctgct
    29881 gagcggcgat gatgcgagcg cgctggactt catgcaatta ggcggtcatg gggttatttc
    29941 cgttacggct aacgtcgcag cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg
    30001 gcattttgcc gaggcacgcg ttattaatca gcgtctgatg ccattacaca acaaactatt
    30061 tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac
    30121 cgatacgctg cgcctgccaa tgacaccaat caccgacagt ggtcgtgaga cggtcagagc
    30181 ggcgcttaag catgccggtt tgctgtaaag tttagggaga tttgatggct tactctgttc
    30241 aaaagtcgcg cctggcaaag gttgcgggtg tttcgcttgt tttattactc gctgcctgta
    30301 gttctgactc acgctataag cgtcaggtca gtggtgatga agcctacctg gaagcggcac
    30361 cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc ggtgattatg
    30421 caatcccggt gaccaacggt agtggtgctg tcggtaaggc gctggacatt cgtccaccag
    30481 cccagccgct ggcactagtt tctggcgcgc gtacccagtt cacgggcgat accgcttcat
    30541 tgctggtgga aaatggtcgt ggcaatactc tgtggccgca ggtggttagc gtgctgcagg
    30601 cgaaaaacta caccatcacc caacgtgatg atgctggtca gacactgacc accgattggg
    30661 tacaatggaa ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa atctctgtta
    30721 agccgcaggg ttatcagcag gcggttacgg ttaaactgct gaacctggaa caggcgggca
    30781 aaccggttgc agacgcggct tccatgcagc gttacagcac ggagatgatg aacgttattt
    30841 ccgccggtct ggataaatct gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca
    30901 ccactatgga cgtacaaagt gcagctgatg acaccggttt accaatgctg gtcgtacgcg
    30961 ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag
    31021 tgaccgacag cacccgttcg cagggcaaca tggccgtaac ttataagccg ctgtctgaca
    31081 gcgactggca ggaactgggc gcgagcgatc caggcctggc atccggtgac tataaactgc
    31141 aggttggcga tttagataac cgcagcagcc tacagttcat cgatccgaaa ggtcatactc
    31201 tgactcagag tcagaacgac gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa
    31261 aatacagggc tggaatcatc cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg
    31321 gcaggaaaac gcgattttag cggtaattcg cacgaaattt gtttgtcgga cgtagttcgg
    31381 ataaggcgtt cacgccgcat ccgacaaaac atccggcaca ccagacagca aaagatttta
    31441 aaacgttaat tcacacccag gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg
    31501 taaagcgaaa accgtataca gcacggaaaa cccggacctg ttggtgctcg aattccgcaa
    31561 tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa
    31621 caacaagttc aactacttca ttatgagcaa actggctgaa gcgggtatcc cgactcaaat
    31681 ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag ctggatatgg taccggttga
    31741 gtgtgtcgtg cgtaaccgtg ctgcgggctc tctggtgaaa cgtcttggaa tcgaagaagg
    31801 tattgagctg aacccgccgc tgttcgatct gtttctgaaa aacgacgcca tgcacgatcc
    31861 gatggtcaac gaatcttact gcgaaacctt tggctgggta agcaaagaga acctggcgcg
    31921 tatgaaagag ctgacctaca aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg
    31981 tctgattctg gtcgacttca agctggaatt tggtctgtac aaaggcgaag tggtactggg
    32041 tgatgagttc tccccggacg gtagccgcct gtgggacaaa gaaacgctgg agaaaatgga
    32101 caaagaccgt ttccgccaga gcctcggtgg cctgatcgag gcctatgaag ccgtcgcccg
    32161 ccgcctgggt gtacagctgg actgattttt ctgttcatca tcttgccgtg ctgctggcac
    32221 ggcaagacaa ccattctcgt aagatgtgca ttgaacgctc attcctccgc catttcatcc
    32281 cgctttcctg tggtaatctt cctctgaacc acctacgatc atttctataa tgaatatatt
    32341 gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg acaatgttga agacaggcgc
    32401 aacagctctg gtggtccgtc tatgggcggt cccggttttc gcctgccgag cggtaaaggc
    32461 gggctgattt tactgatagt cgtgctggtt gcaggctact atggcgttga tttaaccggg
    32521 ttgatgaccg gacagccggt ttcccaacag caatcaacgc ggtcaattag cccaaatgaa
    32581 gacgaagccg caaaattcac ctcggtgatt ctggcaacta cggaagacac ctggggacaa
    32641 cagttcgaga agatgggtaa gacctatcag caaccgaagc tggtcatgta ccgtggaatg
    32701 acgcgtaccg gatgcggggc gggccagtcc ataatggggc cgttctattg cccggcggat
    32761 ggcacggttt atatcgatct ctccttctat gatgacatga aagacaaact tggcgcggat
    32821 ggcgattttg cccaggggta cgttatcgcc catgaagtcg gtcatcatgt gcagaaactg
    32881 ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg cgacgcaggc ggaagtgaac
    32941 cgcttatctg tgcgtatgga actccaggcc gactgttttg ccggtgtctg ggggcatagt
    33001 atgcagcagc aaggcgttct ggaaaccggc gatctggaag aggcgctgaa cgcggcgcag
    33061 gccatcggcg atgaccgttt acaacagcaa agtcaggggc gagtagtacc agacagtttc
    33121 actcatggca cttctcagca acgctacagc tggtttaaac gtggtttcga cagcggcgat
    33181 ccggcacaat gcaatacttt tggtaaaagc atttaacttc cggggcaggg atggctgaac
    33241 tgactgcgct tcacacatta acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg
    33301 tgttgagcgg ggaagagggt tggtgttttg atcatgcgct taagttacgt gatgccttac
    33361 ctggcgactg gctgtggatt tcgccgcagc cagatgctga aaaccactgt tctccctcgg
    33421 cactacaaac tttacttggg cgcgagttcc ggcatgcggt attcgacgcc cgctacggct
    33481 ttgatgccgc tgcctttgcg gcacttagcg gaacgttgaa agcgggaagc tggctggttt
    33541 tgttactccc tgtatgggaa gagtgggaaa accaacctga tgccgactcg ctgcgctgga
    33601 gtgattgccc tgaccctatt gcgacgccgc attttgtcca gcatttcaaa cgcgtactta
    33661 cggcggataa cgacgctatc ctctggcggc aaaaccagcc gttctcgttg gcgcatttta
    33721 ctccccgtac tgactggcac cccgcgaccg gcgcaccaca accagaacaa cagcaactct
    33781 tacagcagtt actgaccatg ccgtcgggcg tggcagcggt aactgctgcg cgtgggcgcg
    33841 gtaagtcggc gttggcaggg caactcattt ctcgtattgc gggcagtgcg attgtcaccg
    33901 cgcccgcaaa agcggcaacg gatgtactgg cacaatttgc gggcgagaag tttcgcttta
    33961 ttgcgccgga tgccttgtta gccagcgatg agcaagccga ctggctggtg gtcgacgaag
    34021 ccgcagccat acctgcgccg ttgttgcatc aactggtatc gcgttttcct cgaacgttgt
    34081 taaccactac ggtgcagggc tacgaaggca ccggacgtgg ttttttgctg aaattttgcg
    34141 ctcgctttcc gcatttacac cgttttgaac tgcaacagcc gatccgctgg gcacagggat
    34201 gcccgctgga aaaaatggtt agtaatgcac tggtttttga cgatgaaaac ttcacccata
    34261 caccacaagg caatattgtc atttccgcat ttgaacagac gttatggcga agtgaaccag
    34321 aaacgccgtt aaaggtttat cagttattgt ctggtgcgca ctaccggact tcgccgctgg
    34381 atttacgccg catgatggat gcaccagggc aacatttttt acaggcggct ggcgaaaacg
    34441 agattgccgg ggcgctgtgg ctggtggatg aggggggatt atctcaacaa ctcagtcagg
    34501 cggtatgggc aggttttcgt cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc
    34561 acggcagcaa tccactggcg gcgacattgc gcggacggcg ggtcagccgg atagcagttc
    34621 atccggctcg tcagcgggaa ggcacaggac agcaacttat tgctggtgct ttgcaatata
    34681 cgcaagacct cgactatctt tcggtgagtt ttggttacac cggggagtta tggcgtttct
    34741 ggcaacgctg cggttttgtg ctggtgcgaa tgggtaatca tcgtgaagcc agcagcggtt
    34801 gctatacggc gatggcgctg ttaccgatga gtgatgcggg taaacagctg gctgaacgtg
    34861 agcattaccg tttacgtcgc gatgcgcaag ctctcgcgca gtggaatggc gaaatgcttc
    34921 ctgttgatcc actaaacgat atcgtgcttt ctgacgacga ctggcttgaa ctggccggtt
    34981 ttgctttcgc tcatcgtccg ctattaacat cgttaggttg cttattgcgt ctgctacaaa
    35041 ccagtgaact ggcattaccg gcgctgcgtg ggcgtttaca gaaaaacgcc agtgacgcgc
    35101 agttatgtac cacacttaaa ctttcaggcc gcaagatgtt actggtccgt cagcgggaag
    35161 aggccgcgca ggcactgttc gcacttaatg atgttcgcac tgagcgtctg cgcgatcgca
    35221 taacgcaatg gcaatttttt cactgactcc ttcaattttc tcccatggtc attgtgagtc
    35281 agcggcgtaa aattaatgac atcagttaag gagttcgcca tgaaacatga ccattttgtt
    35341 gttcaaagcc cggataaacc tgcgcaacag ttgttactgc ttttccacgg tgtcggggat
    35401 aacccggtgg caatggggga aataggcagc tggtttgcgc cgctatttcc tgatgcgctg
    35461 gtggtgagtg tcggaggcgc ggagccgagc ggtaatccgg cggggcgtca gtggttttcg
    35521 gtgcagggta ttacggaaga taatcgccag gcgcgtgtgg atgcgatcat gccgacgttt
    35581 attgaaacgg tacgctactg gcagaaacag agtggagtag gggctaacgc cacggcgcta
    35641 attggtttct cacaaggcgc gattatggcg ctggagagca ttaaagccga accgggcctt
    35701 gcctcacgcg tcattgcctt taacggacgc tatgcgagcc tgccggaaac cgcgtctaca
    35761 gccacgacaa ttcacctgat tcatggcggt gaagatccgg ttatcgatct ggcgcatgca
    35821 gttgcggcgc aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag
    35881 gatttaggcc atgcgatcga caatcgcagt atgcaatttg ccctggatca cttgcgttat
    35941 accattccga aacattattt cgacgaagcg ctaagcggcg gtaagcctgg cgatgatgat
    36001 gtgattgaaa tgatgtaata atagccgggt aggcctgata agaccgtatc agacatcatg
    36061 gccggatgcg gcgttaacgc cttatccggc cactcagctt acttcttcgg ttgatccttt
    36121 ttcggccagt cgtcgtcatc gtcccactta tcgttgaaat cacgatgcgg gggaagctcc
    36181 ggcttgttgg cgagaaattt cttatggtca acgcgcttga gatctttaat cacgttcagc
    36241 aggacgccta ccaaaaaaac aatcaccaga atccaccaat atttagccag ccagtccatg
    36301 cttatttcct cttgcagaac cactcatcac gcgacgagct gttccatgat acgttgatac
    36361 atacgggcaa gtagctgcag gtcggcagcg ttcacacatt cattaatttt atgaatagtg
    36421 gcattgaccg gcccgagttc caccacctgc gcccccatgc gggcaataaa gcgcccgtcg
    36481 gacgttccgc ctgtggtcag tagctgcggt ttaatttcat tatagtgctc aaccgcgtta
    36541 acgaccgcat ccaccagttt accgcgcgcg gtcaaaaatg gctgcccgga aagccaccaa
    36601 tccaccgtat agcgcagttg atgtttttca agcagggcaa gcacctgcgc tttgatcatc
    36661 tcatcagtca gttcggtgct gaagcggaag ttaaactgca caaacagttc acccggaata
    36721 acgttgttac tgcccgttcc cgcctgaata ttggcaatct gcatactggt cgccgggaaa
    36781 aattcattgc cctgatccca ctcaatagcc actaattcat taaggaaagg tgctgcgcga
    36841 tgtaccggat tgtcagccag atgtgggtag gcaacatgcc cctgaacgcc atgaatggta
    36901 aggttgcagg ttaacgatcc gcgacgacca tttttcacca catcacctac cacttcgata
    36961 ctcgacggtt cgccaaccag gcagtaatcg agacgctcat tacgtgccat taacgcttcg
    37021 acgactttta ccgtaccgtt gtgggcactg gcttcttcat cagaggtgat cagaaatgcc
    37081 agtcgccccg tatggttggg atgttgtgcg acaaaacgtt ctgcagctac caccatcgcc
    37141 gccagcgagc ctttcatatc tgccgcaccg cgcccgaata acatgccgtc acgaatggtt
    37201 ggttcaaacg gcggattgat ccaacgatcg gcgtcgccag gcggcaccac gtcggtatgc
    37261 ccggcaaagg ctaacgtttc accttgccca cgccatgccc aaaaattctg cgtatcggca
    37321 aagtccatgc gttcaacggt aaaaccgatc gcctgcaaac gttcaatcaa caaagcctgg
    37381 catcctgcgt catcaggact cagggaaggg cggcgaataa gctgttgtgt cagctcaata
    37441 accgggcacg acatagacta cacctcatgg aaaaattgct gataactgga atcactgaaa
    37501 cccagcagca taggcttacc aggcgcgcag agcaatggac gtttgataat tgcaggcatt
    37561 tcagtcatta atgccgccgc agaggccgca tcggtgattt tattgcgggt ggtttcgtcc
    37621 agtttacgcc aggttgtacc acgggtgttg agtaacgctt cccagcctaa ttcgttgata
    37681 aaaccgttca ataattcgct gtccagccca tcgacgcggt aatcatgaaa acgatagtcg
    37741 atgttattgg cttccagcca acggcgagcc tttttaatgg tgtcacaatt tttgatgccg
    37801 taaagtgtaa ccataatgaa tcctttttcg attta
//
LOCUS       DAZGBC010000026        37631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGBC010000026 DAZGBC010000000
VERSION     DAZGBC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.026"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1234
                     /locus_tag="ACHK1J_001360"
     CDS             <1..1234
                     /locus_tag="ACHK1J_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7910660.1"
                     /translation="VKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILS
                     LAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGK
                     DNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINY
                     TKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISR
                     GIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKD
                     FCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTE
                     LNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADK
                     ALREAKSEGGNKVIIHHI"
     gene            1415..2905
                     /gene="dgcJ"
                     /locus_tag="ACHK1J_001361"
     CDS             1415..2905
                     /gene="dgcJ"
                     /locus_tag="ACHK1J_001361"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO7910661.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2948..3451
                     /gene="yeaK"
                     /locus_tag="ACHK1J_001362"
     CDS             2948..3451
                     /gene="yeaK"
                     /locus_tag="ACHK1J_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO7910662.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3452..3556)
                     /gene="yoaI"
                     /locus_tag="ACHK1J_001363"
     CDS             complement(3452..3556)
                     /gene="yoaI"
                     /locus_tag="ACHK1J_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO7910663.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3726..4172
                     /gene="yeaL"
                     /locus_tag="ACHK1J_001364"
     CDS             3726..4172
                     /gene="yeaL"
                     /locus_tag="ACHK1J_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO7910664.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4129..4950)
                     /gene="nimR"
                     /locus_tag="ACHK1J_001365"
     CDS             complement(4129..4950)
                     /gene="nimR"
                     /locus_tag="ACHK1J_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO7910665.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5047..6228
                     /gene="nimT"
                     /locus_tag="ACHK1J_001366"
     CDS             5047..6228
                     /gene="nimT"
                     /locus_tag="ACHK1J_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO7910666.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6283..6630
                     /gene="yeaO"
                     /locus_tag="ACHK1J_001367"
     CDS             6283..6630
                     /gene="yeaO"
                     /locus_tag="ACHK1J_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO7910667.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6652..6906)
                     /gene="yoaF"
                     /locus_tag="ACHK1J_001368"
     CDS             complement(6652..6906)
                     /gene="yoaF"
                     /locus_tag="ACHK1J_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO7910668.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTTQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7089..8114
                     /gene="dgcP"
                     /locus_tag="ACHK1J_001369"
     CDS             7089..8114
                     /gene="dgcP"
                     /locus_tag="ACHK1J_001369"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO7910669.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8147..8245
                     /gene="yoaK"
                     /locus_tag="ACHK1J_001370"
     CDS             8147..8245
                     /gene="yoaK"
                     /locus_tag="ACHK1J_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO7910670.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8248..8322
                     /gene="yoaJ"
                     /locus_tag="ACHK1J_001371"
     CDS             8248..8322
                     /gene="yoaJ"
                     /locus_tag="ACHK1J_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO7910671.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8381..8629)
                     /gene="yeaQ"
                     /locus_tag="ACHK1J_001372"
     CDS             complement(8381..8629)
                     /gene="yeaQ"
                     /locus_tag="ACHK1J_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7910672.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8777..8959)
                     /gene="yoaG"
                     /locus_tag="ACHK1J_001373"
     CDS             complement(8777..8959)
                     /gene="yoaG"
                     /locus_tag="ACHK1J_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO7910673.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8963..9322)
                     /gene="yeaR"
                     /locus_tag="ACHK1J_001374"
     CDS             complement(8963..9322)
                     /gene="yeaR"
                     /locus_tag="ACHK1J_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO7910674.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9495..10133)
                     /gene="leuE"
                     /locus_tag="ACHK1J_001375"
     CDS             complement(9495..10133)
                     /gene="leuE"
                     /locus_tag="ACHK1J_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO7910675.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10260..11183)
                     /gene="dmlR"
                     /locus_tag="ACHK1J_001376"
     CDS             complement(10260..11183)
                     /gene="dmlR"
                     /locus_tag="ACHK1J_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO7910676.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFATVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQLILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11286..12371
                     /gene="dmlA"
                     /locus_tag="ACHK1J_001377"
     CDS             11286..12371
                     /gene="dmlA"
                     /locus_tag="ACHK1J_001377"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO7910677.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12622..14232
                     /gene="yeaV"
                     /locus_tag="ACHK1J_001378"
     CDS             12622..14232
                     /gene="yeaV"
                     /locus_tag="ACHK1J_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO7910678.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGALLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14264..15388
                     /gene="yeaW"
                     /locus_tag="ACHK1J_001379"
     CDS             14264..15388
                     /gene="yeaW"
                     /locus_tag="ACHK1J_001379"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO7910679.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15444..16409
                     /gene="yeaX"
                     /locus_tag="ACHK1J_001380"
     CDS             15444..16409
                     /gene="yeaX"
                     /locus_tag="ACHK1J_001380"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO7910680.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPLDTTHYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKIGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16463..17578)
                     /gene="rnd"
                     /locus_tag="ACHK1J_001381"
     CDS             complement(16463..17578)
                     /gene="rnd"
                     /locus_tag="ACHK1J_001381"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO7910681.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17660..19345)
                     /gene="fadD"
                     /locus_tag="ACHK1J_001382"
     CDS             complement(17660..19345)
                     /gene="fadD"
                     /locus_tag="ACHK1J_001382"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO7910682.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19550..20131)
                     /gene="yeaY"
                     /locus_tag="ACHK1J_001383"
     CDS             complement(19550..20131)
                     /gene="yeaY"
                     /locus_tag="ACHK1J_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO7910683.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20171..20866)
                     /gene="tsaB"
                     /locus_tag="ACHK1J_001384"
     CDS             complement(20171..20866)
                     /gene="tsaB"
                     /locus_tag="ACHK1J_001384"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO7910684.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLSDIDALAYGRGPGGFTGVRIGIGIAQGLALGAALPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGVWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20924..22834)
                     /gene="yoaA"
                     /locus_tag="ACHK1J_001385"
     CDS             complement(20924..22834)
                     /gene="yoaA"
                     /locus_tag="ACHK1J_001385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO7910685.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     KLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22966..23310
                     /gene="yoaB"
                     /locus_tag="ACHK1J_001386"
     CDS             22966..23310
                     /gene="yoaB"
                     /locus_tag="ACHK1J_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7910686.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            23732..24031
                     /gene="yoaC"
                     /locus_tag="ACHK1J_001387"
     CDS             23732..24031
                     /gene="yoaC"
                     /locus_tag="ACHK1J_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO7910687.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(24151..24330)
                     /gene="yoaH"
                     /locus_tag="ACHK1J_001388"
     CDS             complement(24151..24330)
                     /gene="yoaH"
                     /locus_tag="ACHK1J_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO7910688.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            24404..25765
                     /gene="pabB"
                     /locus_tag="ACHK1J_001389"
     CDS             24404..25765
                     /gene="pabB"
                     /locus_tag="ACHK1J_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO7910689.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            25769..26347
                     /gene="nudL"
                     /locus_tag="ACHK1J_001390"
     CDS             25769..26347
                     /gene="nudL"
                     /locus_tag="ACHK1J_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO7910690.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            26531..27895
                     /gene="sdaA"
                     /locus_tag="ACHK1J_001391"
     CDS             26531..27895
                     /gene="sdaA"
                     /locus_tag="ACHK1J_001391"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7910691.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            28026..29624
                     /gene="pdeD"
                     /locus_tag="ACHK1J_001392"
     CDS             28026..29624
                     /gene="pdeD"
                     /locus_tag="ACHK1J_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7910692.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(29628..31184)
                     /gene="yoaE"
                     /locus_tag="ACHK1J_001393"
     CDS             complement(29628..31184)
                     /gene="yoaE"
                     /locus_tag="ACHK1J_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO7910693.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(31172..31330)
                     /gene="yoaL"
                     /locus_tag="ACHK1J_001394"
     CDS             complement(31172..31330)
                     /gene="yoaL"
                     /locus_tag="ACHK1J_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO7910694.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            31647..32618
                     /gene="manX"
                     /locus_tag="ACHK1J_001395"
     CDS             31647..32618
                     /gene="manX"
                     /locus_tag="ACHK1J_001395"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO7910695.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            32681..33481
                     /gene="manY"
                     /locus_tag="ACHK1J_001396"
     CDS             32681..33481
                     /gene="manY"
                     /locus_tag="ACHK1J_001396"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO7910696.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            33494..34345
                     /gene="manZ"
                     /locus_tag="ACHK1J_001397"
     CDS             33494..34345
                     /gene="manZ"
                     /locus_tag="ACHK1J_001397"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO7910697.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            34400..34858
                     /gene="yobD"
                     /locus_tag="ACHK1J_001398"
     CDS             34400..34858
                     /gene="yobD"
                     /locus_tag="ACHK1J_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO7910698.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            35169..35291
                     /locus_tag="ACHK1J_001399"
     CDS             35169..35291
                     /locus_tag="ACHK1J_001399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910699.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            35288..35854
                     /gene="mntP"
                     /locus_tag="ACHK1J_001400"
     CDS             35288..35854
                     /gene="mntP"
                     /locus_tag="ACHK1J_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO7910700.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(35851..36660)
                     /gene="rlmA"
                     /locus_tag="ACHK1J_001401"
     CDS             complement(35851..36660)
                     /gene="rlmA"
                     /locus_tag="ACHK1J_001401"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO7910701.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(36826..37035)
                     /gene="cspE"
                     /locus_tag="ACHK1J_001402"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(36826..37035)
                     /gene="cspE"
                     /locus_tag="ACHK1J_001402"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7910702.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(37048..37191)
                     /gene="yobF"
                     /locus_tag="ACHK1J_001403"
     CDS             complement(37048..37191)
                     /gene="yobF"
                     /locus_tag="ACHK1J_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO7910703.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         9297 a         9505 c         9180 g         9649 t
ORIGIN      
        1 tgtaaaactc atcccacaga tgaattacct gatggttgtt gtggctttgt ttttccttaa
       61 cgccgtcatt tttcttttca tgttaatgaa atatttcact aacaaacaaa ttttaccaac
      121 actcatttta agccttgcat ttttaagtgg ccttatctat ttagttgaaa ccattgtaat
      181 tatccataaa ccaattaacg gcagtacact gatccagaca aagtcgaatg atgtttctat
      241 tttctatatt ttccgccaac tcagttttat ttgtttaacc tcgctggcgc tcttttgtta
      301 tggaaaagac aacatccttg acaacaataa gaaaaaaact ggcattctgt tgctggcgct
      361 gattcctttt ttagtttttc cccttctggc acacaatctg agcagttata acgctgacta
      421 ttctttgtat gtcgtcgatt actgtccgga caaccatact gcgacctggg gaatcaacta
      481 tacaaaaata ttggtttgtc tgtgggcatt tttactgttc tttattatca tgcgtacacg
      541 attagccagc gaactatggc cgttaatagc attattatgt ctggcatcgc tatgctgcaa
      601 cttacttcta ctgactctgg atgagtataa ttataccatc tggtatatca gtcgcgggat
      661 tgaagtctcc agtaaactgt ttgttgtgtc ttttctgatt tataacattt ttcaggaact
      721 gcaactctcc agcaaactgg cagttcatga tgtgctgacc aatatttata atcggcgcta
      781 ctttttcaac agcgtagagt cattattgtc gcgacctgtt gttaaggact tctgtgtcat
      841 gctggttgat attaatcagt tcaaacgcat caatgcccaa tggggacatc gtgtgggtga
      901 taaagtgctg gtttcaattg tcgatattat ccagcaaagc atccgccccg atgatatttt
      961 agcgcgactg gagggtgagg tgtttggctt gctatttacc gaactcaata gtgcccaggc
     1021 aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc ctgaccggct ttagtaacag
     1081 atatgatgtt cctgaacaaa tgaccatcag tattggcacg gttttttcaa cgggtgacac
     1141 gcgtaatatc tcgcttgtca tgacggaagc agataaagcc ttacgcgaag cgaaaagcga
     1201 ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa aaatattcag agccatgctt
     1261 tttgcgtggc ttttgcatac aatttattac tattacccct aaatttcccc tccccagcgg
     1321 cgcagagatg agtataatta gcgcccctgt gccaggccgc aatcgaactt tatctggttt
     1381 tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa ttgcaccata gaatgctccg
     1441 gcattttatc gccgcaagtg tcattgtgct gacatcttcc ttccttattt ttgaacttgt
     1501 cgccagcgac agagcaatga gtgcctatct gcgctatatc gtgcagaaag cagactcctc
     1561 ctttctttat gataagtatc agaatcagag tattgccgcg catgtgatgc gcgctctcgc
     1621 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
     1681 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
     1741 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
     1801 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
     1861 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
     1921 gccggttaat gttgaatact ttgctatgaa taactggtcc ttcctgcagt caggaagtat
     1981 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
     2041 ttaccaggat cagcgtacta aacagaatgt gatgagtttg ctgacgccgg tgtatgttgc
     2101 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatctgc ggaatatctt
     2161 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgatacaga
     2221 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
     2281 cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
     2341 cacgtcttcg tggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
     2401 ggtgcgaatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
     2461 gactggactg tataatcgca aaattttaac tcctgaactg gagcagcggt tgcagaaact
     2521 ggtgcaatcc ggttcttcgg tgatgtttat tgctattgac atggacaagt taaagcaaat
     2581 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttattag ctcaggcgat
     2641 taaacaatcg attcgtaaaa gtgattatgc catccgactc ggtggcgatg aattctgcat
     2701 cattcttgtc gattcgacgc cgcaaattgc agcacaactg cctgaacgta tcgaaaaacg
     2761 tctgcaacat atcgcgccgc agaaagagat cggcttctct tccggtattt acgcgatgaa
     2821 agaaaacgat acgttacatg atgcgtataa agcttccgat gagcgtttat atgtcaataa
     2881 gcagaacaaa aacagccgtt catgataacc ttctgtggtt gtttgcttgt aatctcagga
     2941 gcgtgaaatg actgaaatgg ctaaaggaag cgtgacgcat cagcggttaa tcgctttatt
     3001 atcacaagaa ggtgcagact tccgcgtcgt tacccatgaa gcggtaggta aatgtgaagc
     3061 ggtgtcagaa attcgtggca ccgctctagg ccagggtgct aaggcactgg tatgtaaagt
     3121 caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc gcagccgatc aacaggccga
     3181 tctgagccaa ctcgccagcc atataggcgg attacgcgct tcgttggcca gcccagcgga
     3241 agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc cctttcagct tccatccaaa
     3301 actcaaactg gttgccgacc ctctactctt cgaacgattt gatgaaatcg ccttcaatgc
     3361 agggatgctg gataaatccg ttattttgaa aaccgcggat tatttgcgca ttgcgcaacc
     3421 agaactggtc aatttccgcc gcactgcgta gctagcctgt ccgttcaata agcagaacgg
     3481 acaaaaccag cacaaccgca atggcaaaaa acgatgacgt gataatcagt gtctcgacga
     3541 acatttgatc gttcatagca tgcccccggg agatagctgt tttcacgtta ttgttagcgt
     3601 gcacaaatgg cagtttgatg acagttcgcc tgatttttta tgcaaaaaac gtaaagattt
     3661 tctacttcct tcctgcagca agcgtaaagt aagcaggctt attatttttt ggcaaggaaa
     3721 ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt agctgcgctg ggctttatca
     3781 gtcataacac cactgtcgcc gtttcaattc tggtgttgat cattgtccgc gtcacaccgt
     3841 taagcacctt tttcccgtgg attgaaaaac aggggctaag tatcgggata atcattctga
     3901 cgattggcgt tatggccccc atcgccagcg ggacgctacc gccctcaacc ttgattcact
     3961 cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg cgttattgtc tcctggctgg
     4021 gtgggcgcgg cgtgacgtta atgggcagcc agccgcaact ggttgctggg ctgctggtgg
     4081 gcactgtttt aggcgtagcg ctgtttcgcg gcgtaccggt tggcccgctt attgccgcgg
     4141 gtctggtttc gctgattgtg gggaaacagt agttaatccc gcaatatatc ggcctggcgt
     4201 ttgccccagg cctttcttaa acatagtaat aaaggccgtt gttgaatcat accccagcgt
     4261 atgagcaact ttctgtacag tatcgccttt cactaatccc tgaagcgcca taatcagctg
     4321 taactgctga cgccactggc gaaaactcaa ccccgtctct tttactatca gtcgggcaag
     4381 attgcgttca ctcatcgcaa aaaagccagc ccattgcccc aatgaccccc actcgacggg
     4441 ccctttcgcc atcatctcca ccatcgtgcg gattttagga tgagaagaaa cgggcaggtg
     4501 cagctgttgt tgtggctgct gagggagttc atcaaacagg acctgaatta atctgcgggt
     4561 catcggctca gcacgctgag tcgtcgtcct gtttgccagg gttaaaatca attcccggca
     4621 taagggcgat attttcaatg tgcaacatgt tgtcggcatt gtcactgcgc tgggttcgat
     4681 aaacagaaaa cagagttcag cgttagctgt cacttgattg ctatgttcca ccccgcccgg
     4741 tatccaaacc gcatattgcg gcggcaccat ccacaaagca ttttccaccg tacaggtaat
     4801 tgcaccatgt agcgccagta tcagctgccc tttgcggtgc tgatgtacag gactggttaa
     4861 ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct tcatggtgtc ggtcgggttc
     4921 atagccattg agattcaacc tatgcatcat tttgtccgaa tttagcgata atttgtcatt
     4981 ttagcttgat tcaacataac aataaaaacg gtaaggtaca gcctcgtttg taacaatgag
     5041 aagcatatga cctgttcaac ttcattaagc ggcaaaaaca ggattgtcct tatcgctggc
     5101 attctaatga ttgccacaac attacgcgtc acctttaccg gcgcagcacc gttactggat
     5161 acgattcgtt ccgcttactc gctgacgacg gcgcaaaccg gcttattgac caccctgcca
     5221 ttattagcct ttgcgctaat ctcacctctg gctgccccgg tggcgcgacg ttttggtatg
     5281 gaacgtagcc tgtttgccgc gttacttttg atctgtgctg gtatcgcaat tcgctctctc
     5341 ccttcgcctt acttattatt tggcggtaca gcggtcattg gcggtggaat tgcattaggc
     5401 aatgtcttac tgccaggatt aattaaacgc gatttccctc attccgtcgc cagacttacc
     5461 ggcgcatatt ccctgacaat gggagctgca gcggcactgg gatcggctat ggtcgtgccg
     5521 ctggctttga acggttttgg ctggcaaggc gcgttgctca tgctgatgtc ttttcctctg
     5581 ctggctcttt ttttatggct gccacagtgg cgaagtcaac aacatgcaaa tttgagtacc
     5641 tcacgcgcct tacatactcg gggtatctgg cgttctccgc ttgcctggca ggtaacattg
     5701 tttcttggga tcaactcact ggtctattac gtgattattg gctggcttcc agcgatcctc
     5761 atcagtcacg gctatagcga agcacaggcg ggttcactgc atggtttgct gcaactagcc
     5821 acagcagctc ccggtttgct gatcccactt ttcttacatc atgtgaaaga tcagcgtggt
     5881 attgcagcgt tcgttgcctt gatgtgcgca gtgggcgcgg tcgggctctg ctttatgcca
     5941 gcgcacgcga tcacctggac tctgcttttc ggttttggtt ccggcgcaac aatgatactg
     6001 gggttgacgt tcattggtct tcgggcaagt tctgcgcatc aggcggcggc actctcgggg
     6061 atggcacaat ccgtcgggta tttgctggca gcctgtgggc cgccgctgat gggtaaaata
     6121 cacgatgcta acggtgactg gtccgtacca ctgatggggg ttgcaatact ttcactactg
     6181 atggcgattt tcggactttg cgccgggaga gacaaagaaa ttcgctaata tccggtgcta
     6241 tagtgacgtg accaatcatg cgtggaaggg agaacaaaca cgatgaatat tcagtgcaaa
     6301 cgcgtttatg atccggcaga acagagcgat ggttatcgcg tactggtcga ccgcctctgg
     6361 ccgcgtggta tcaaaaaaac cgatttagcc cttgatgagt gggataaaga aatcacgccg
     6421 tcaacggaac tgcgcaaagc ctttcacggc gaagtcgtcg attttgcaac ctttcgcgag
     6481 caatatcttg cagaactggc gcaacacgag caagaaggaa agcggctggc ggacatcgcc
     6541 aaaaaacagc cgctgaccct gctttactca gcaaaaaaca ccacgcagaa ccatgcgctg
     6601 gtgctggccg actggctacg tagcttgtga ttttagtaca gcatccggcg gttatttttc
     6661 accagccgga tggtcccgtc gccacaatgc ccattcatca atcgtttcac cgcccggtaa
     6721 tttgcaattg ttgctgaccc cttgcgttgt ctgcactgga atgagcgtcc cgcccttctg
     6781 ctggcaatag accgacgccg gatttgccat accaatctgc ggcggtttag gtgcttctgg
     6841 ctgagaagag gttgaacaac cagccagaac cagcaagcaa ggcagaacaa aactgataat
     6901 tttcatttat tgatctcaca tatttatcca ggcttagagt atcgcggtat cgttttgttt
     6961 tgcagcacta tttttattac attcactcaa atcatattac gccttgtttc atctttgttg
     7021 atgatgtttt atcatgcctg caaagattaa ataatcagca tttacccgcc gtatcctgga
     7081 gttgtttcgt gtcagatcag attatcgccc gcgtctcgca atcccttgcc aaagaacagt
     7141 cactggaaag cctggtccga cagcttctgg agatgctgga aatggtcact gatatggaat
     7201 caacctacct gaccaaagtg gatgtcgaag cgcgcctgca gcatataatg tttgcccgta
     7261 acagccagaa aatgcacatc ccggagaatt ttaccgtctc gtgggattac tcgttatgca
     7321 aacgcgccat tgatgaaaac tgctttttca gcgatgaagt ccccgaccgt tggggcgact
     7381 gtattgcggc acgcaatctt ggcatcacca catttctgag cacgccaatt cacttaccgg
     7441 atggatcatt ctatggcacg ctttgcgccg ccagcagtga gaagcgccag tggagtgaac
     7501 gcgcggaaca ggttttgcag ttattcgccg gactgattgc acaatatatt caaaaagagg
     7561 cgctggtaga acagctgcgc gaagccaatg ccgcgctgat agcgcaatcg tataccgact
     7621 cgttaaccgg gttaccaaat cggcgggcga tttttgaaaa tctgacgacg ctgttttctc
     7681 tcgcccggca tcttaaccat aagataatga tcgcgtttat cgacctggat aacttcaaat
     7741 taatcaacga tcgttttggt cataatagtg gcgatctgtt tctcattcag gttggcgagc
     7801 gccttaatac gctccagcaa aatggcgaag ttattggtcg tctcggcggt gatgagtttt
     7861 tggttgtttc actaaacaac gagaatgcgg atatttcgtc gctgcgagaa cgcattcagc
     7921 agcaaatacg cggagaatat cacttaggtg atgttgattt gtattatcct ggtgccagtc
     7981 ttggcatagt agaagtcgat cctgaaacga ccgatgcaga cagtgcccta catgctgccg
     8041 atatcgcgat gtatcaggag aaaaaacaca aacagaaaac accttttgtc gcgcattcag
     8101 cgccacattc ctgaggcgta ttcacatcct tttgattggt gataacatgc gaatcggtat
     8161 tatttttccg gttgtaatct tcattacagc ggtcgtattt ttagcatggt tttttattgg
     8221 cggctatgct gccccgggag cataaagatg aaaaaaacaa cgatcattat gatgggtgtg
     8281 gcgattattg tcgtactcgg cactgagctg ggatggtggt aacgtcacat ctaaaaaata
     8341 gcaaaggctg cctgtgtgca gcctttgtgc aatttacgcg ttaactttta atcttcctgt
     8401 agataaatag cacgacaatc gcaccaataa cggcaaccac gaagctgcca aaattgaagc
     8461 catcgacttt accaaagcca aacagcgtgc tgatccaacc gccgactacg gcaccgacta
     8521 tccccagcag gatagtcata aagaatccac caccatcttt acctggcatg atccacttcg
     8581 ccagaatacc ggcaataagc ccaaaaataa tccatgacag aatgcccatt gtttcctcac
     8641 ttatctgttt tgcattagcg ggttagtcgc tgataaaaag catagcacaa catcgggagg
     8701 gcaagatttg tgacgagcat cacggaggat ttttttgcga tggcgcagaa attgcgccat
     8761 caacgatcag tgataattac caaccacaaa catcatgttc gttttccgtg tcataagaac
     8821 gcacggtatt caccagatct tttatcactt cagccgccac ttctggcacc agcaaagtca
     8881 tcggcgtctc tgtttcataa tcgacagaaa cgccattgct gttattggtg acggtcacgg
     8941 tatacgttgc tttgcccatg attcatttcc cgttatgaat gactttccgt tgttgcgcac
     9001 cttccatcag gacttcagga gccacgaaga agtcaatgtt gaaataagta tcgtcagtca
     9061 tggcttcaat gttgtgccac ttttctggag ggaacaccgc aaactgcccc gcttcgataa
     9121 ggatcacctg atcaggctct gcactgtgtt catcagcgta gccgagatat ttgaccgccc
     9181 catgcataac ggaaaggcgt gggtaaaccc ccgggcgcgt tcctttatca agatgacgtt
     9241 cgaatattcc ggcaggcgca gtttgtttat tccagaaagg cgttgagcgc gtatgaatat
     9301 aattctgtgg gatttgcagc atccttttcc ctccttcggt gaatgcgctg aaaacggttt
     9361 attccagccg tttcagggta cgcctgataa tttgcatttt aaataccatt tattggttat
     9421 tttttagcac catatcagag aagaatcagg gaggattata gatgggaagc ccgcgacaac
     9481 gcgggctgaa agcatcagga ttgcagcgtc gccagtcggg cagcgaaacc cacgaacatc
     9541 aaaccaatca gtgagttgcc cactttagcc agtttctttt tggtacgtat gtactgcgtg
     9601 acaaaagcac cagatataat caggaagctc aaatagcaga aactcaccag ttccagcgtc
     9661 gtcgccagaa taaagaatga aattcccgta tgtggggcat taacatcgat aaactgtacg
     9721 aaaaacgaca catagaacaa aatggctttc ggattagtca ggctcaaaat taacgcgcgt
     9781 ttaaaaatgg caccgtattg gggctcatcg gatttggcct cgttattttt acccttcagg
     9841 gtcgcgtaga gaattttact ccccagatag agcaaataaa atgcaccaag ataacgtaca
     9901 atgttgaata atatcggggt ggtcttaatt aatgtcgcca ctccagccca tgccagaaac
     9961 atcaataccg catcgccaat aaatacaccg caggccgcaa gataaccgcc tttcataccg
    10021 ctactgacgc tatttttgag tacaaacagg gtatttggcc ctggcaccaa cacaataaaa
    10081 atggccccaa ccagataggt ccagtaattc agaaccccgt attcagcgaa cacattaacc
    10141 tctttaatta tctttcgatc atgcgcgatt aaaggtgaat atgctaacca atctgtagca
    10201 gcttagaaag gagaaaatca ggttttaacc tgatatcaac ccgataattg aatcattaat
    10261 caggcatgct ccagtgaaaa attcgggtag tgctcggcaa aatactggcg taaaaattct
    10321 accgttatcc gcactttcgc tgacgtcgcc agccttgaaa cataaacggc ccagacgttc
    10381 gctggctggt aatattccgg tagcacttgc actaaatgac cactggcaat gttttcgcta
    10441 acatcccacc aggaacgcag agcaatcccc tgcccatcga gacaccactg atggacaatt
    10501 tctccatgat tagacgacag tgggccggtg actttaatcg catggtggcc ttctttattg
    10561 cgtaattgcc agacaccaaa aggatgatcg cgctctttaa tgaccaggca cggcaatgca
    10621 gaaagatcgg ttaaatgttt cggcgcgcca tgttgcgcaa taaattccgg cgacgcgcag
    10681 agaatgcggt aattggtcgc cagtttgcgg gcgattaaat taggggcgat gtcatcgcca
    10741 atgcgaatat cgagatcgac accttcattg accaaatcga ccagccggtc ttccacatca
    10801 aaacgtaatt ccagttgcgg atacgccttc gccagcgctg ataacgccgg agccaccacc
    10861 tgtcgtccaa aaccaaagct gctgataata cgcagcatcc cctgtggcac ctgacgcacg
    10921 tcagaaagtt cgtccatcat ctgaccgaca tcctgcaaaa tcagctgcgc ccattcataa
    10981 atccgctctc cttcttcggt aatggtgacg cggcgggtgg tgcggtgtaa caacaccacg
    11041 tttagcgttt gctccagcaa ggcgatgcgc ttgctgacga acgccggtga aacgcccagt
    11101 tcttccgcca cggtggcaaa accagcccgg cgagccacca gcataaagac gcgcaaatca
    11161 ttcagcagcg gtaaattatt catgattcgt gttttatgtt tcaccagtta cggggattaa
    11221 ttccttttca gtcaattata ggatggtgat gttgtcaatt ttgatggtca ggaagtgaga
    11281 acccaatgat gaaaacgatg cgtattgctg cgatcccggg agacgggatt ggcaaagaag
    11341 tccttcctga agggattcgc gtgttacagg ctgccgctga gcgctggggc ttcgccttga
    11401 gttttgagca aatggagtgg gcgagctgcg agtattacag ccatcacggt aaaatgatgc
    11461 cggacgactg gcatgagcaa cttagccgtt tcgatgccat ctattttggt gccgtcggct
    11521 ggccggacac cgttccggac catatttcgt tgtggggttc gctgctgaaa tttcgtcgtg
    11581 aattcgacca gtacgtcaac ctgcgcccgg ttcgtctctt tcctggcgtt ccctgcccgc
    11641 tggcgggaaa acagcctggc gacatcgatt tttacgtggt cagggaaaac accgaaggcg
    11701 aatattcctc gctcggcggt agagtgaatg aaggtacaga gcatgaagtc gtcattcagg
    11761 aatcggtatt tacgcgtcgt ggtgtcgatc gcattttgcg ttatgccttc gaacttgcgc
    11821 aaagccgccc acgtaagacg ctaacttctg ctactaaatc aaacggttta gccatcagca
    11881 tgccgtactg ggatgagcga gtggaagcaa tggccgagaa ttacccggag atccgctggg
    11941 acaagcagca tattgatatt ctctgcgcgc gttttgtgat gcagccggaa cgcttcgatg
    12001 tggtggtggc gtccaatttg tttggcgata tcctttccga tcttggcccg gcctgcaccg
    12061 gcaccattgg cattgcccca tccgccaacc tgaatccgga acgcactttc ccgtcgctct
    12121 tcgagcctgt ccacggttcc gcgccggata tctacgggaa aaatattgct aaccctatcg
    12181 ccacaatttg ggccggtgca atgatgctcg attttctcgg caatggcgat gagcgtttcc
    12241 agcaagcgca taacggtatt ctggcagcga ttgaagaagt gattgctcac gggccgaaaa
    12301 caccggatat gaaaggcagt gccaccacgc cacaggttgc cgacgcgatt tgcaaaatta
    12361 ttttgcgtta aggtcaaacc agtttatttg aaccgcgtca ctgacgcggt ttttttattc
    12421 gttctttgca gttaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta
    12481 tggctttccg tattcttaat tgtttaattt atgtaacatg caaataatca atttgttaaa
    12541 cacaacatac aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct
    12601 tttatgccct ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata
    12661 agcatcagcc tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa
    12721 tcggccgacg cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgccgta
    12781 caggtattgg ttttgctggc aatgggactg gtgatttatt tagccaccag taaatacggc
    12841 aatattcgtc ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt
    12901 atttgtgccg gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat
    12961 tatcaaacgc ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtc
    13021 ccttactcct tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta
    13081 atcatggctt atcactttca tgtgcggaaa aacaaaggtc tgagtctttc cggcattatt
    13141 gccgcgatta ccggcgttcg cccgcaaggc ccatggggaa aactggttga tttgatgttc
    13201 ctgatcgcca ctgtcggcgc actgactatt tcccttgttg tcaccgcagc aacctttact
    13261 cgtggacttt ccgcactgac cggtttaccc gataacttca ccgtgcaggc atttgtgatc
    13321 ctgctttccg gcggcatttt ttgcctaagc tcatggattg gtatcaacaa cggtttgcaa
    13381 cgtctgagca aaatggttgg ctggggcgcg ctcctgctgc cattactggt gctgattgtc
    13441 ggtccaaccg aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac
    13501 ttcctgcaaa tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg
    13561 accgttttct actggctgtg gtggatctca tacacccctg gcgtagcaat gtttgtcacc
    13621 cgcgtttccc gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc
    13681 gtcggttgct ggttcttctt cggcgtaatg gaaagctatg ccattcatca gtttatcaat
    13741 ggcgtaatca acgtcccaca ggtgctggaa actctgggcg gcgaaacagc tgtacagcaa
    13801 gttctgatgt cgttgccagc tggtaaattg ttcctcgccg catacctggg cgtgatgatt
    13861 attttccttg cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt
    13921 aatctccagg aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc
    13981 actctgatcc cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc
    14041 gtcgtgctca cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt
    14101 cgctggctga aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg
    14161 cagacaccgg ttgaagccct ggaaaaaaca ccagtgctcc ctgcgggaac cgtattcaaa
    14221 ggcgacaact gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc
    14281 tgactttgta ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc
    14341 ccgtttttat accgatcaga acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag
    14401 ctggatttgc gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcggga
    14461 gatcattggc gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta
    14521 taacgtgtgt ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt
    14581 gattacctgc ccatatcacg catgggcatt caaactcgat ggcaacctgg cccatgcacg
    14641 taactgcgaa aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct
    14701 ggaagaatat gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga
    14761 tcagttaccc ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa
    14821 actggcggcc cgctttacca cccgcacgcc tgccaactgg aagaacattg tcgataacta
    14881 tctcgagtgc tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga
    14941 tcgttactgg cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc
    15001 cgaacagtcg tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg
    15061 gccgtgcacg atgctgaacg tcaccccgat caaagggatg atgacggtca tttatgaatt
    15121 cccggtggat tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagagtt
    15181 aaccgacgag caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt
    15241 acgtctggtt gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg
    15301 catcatggcc gacagtagcg gtagcggcat ttccgaacat ggtatcgccc atttccataa
    15361 tctgctggcg caggtgttta aggactaatg acatcggcgg cggtattttc cgccgctggg
    15421 ctgatttttg atggagtaca gcaatgtcag actatcaaat gtttgaagta caggtgagcc
    15481 aggttgaacc ccttaccgaa caggtgaaac gcttcacgct ggtggcaacc gatggcaaac
    15541 cattacctgc gtttactgga ggaagtcacg tcattgtgca gatgagcgat ggtgataacc
    15601 agtacagcaa tgcgtattca ctgctgagtt cgcctctcga tacaactcat tatcagattg
    15661 ccgttcgact ggaagaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag
    15721 tgggcgatcg gttaacgatc tcaacgccta ataacctgtt tgcgctaatt ccctcagcca
    15781 gaaagcatct gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag
    15841 agttgcaaca cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt
    15901 gcgcatttcg tgatgagcta gtccagcatc cgcaggctga gaaagtccat ttgcatcatt
    15961 catcaaccgg aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac
    16021 acgtttatac ctgtggcccc gaggcgctaa ttgaagcggt aagaagtgaa gctgcgcgtc
    16081 tggacatcgc cgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaatcggcg
    16141 atgcatttac cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga
    16201 ctattttgca ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag
    16261 gggtatgcgg aacctgcgaa acggcaatac tggaaggtga agctgaccat cgggatcagt
    16321 attttagcga tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga
    16381 agggtaaacg cctggtgttg gatttgtagt ttgccgaagc cggatgtggc gctgagcgcg
    16441 cccagtccgg cttcggaaga ttttactgcg gatattcctg caataaattg tgtaatgctt
    16501 ccgccatcag ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca
    16561 gtttccagtg ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc
    16621 tgatcttgtg cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac
    16681 gataacccgg catgtccatc aggttaagca tcggctgcgg taaggcctct tccggcaatg
    16741 tctgcgcttt ttccaccagc gccagcagcg ttttaccgtg aaagcggatt tcgctaccgg
    16801 ataaacccag gctgtccagt tcgcctaaac ttcccggcat ataacgcgct accgaccaca
    16861 aatgctcttc acgcacgaca aagttcaccg ccagatcgcg ctcacgcgcc ttgcgcagtc
    16921 gccagtcggc taacagttgc agacaggcca gttggcgtgt gcgtaattgc caggcattgg
    16981 tgatatcacg ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc
    17041 ggcattcatc cagcgccgca ggtagccagc cggaggcctc cgtttctacc ataagcttgg
    17101 cggtgatcgg taacagatac cagacatccg ccgctgcata ttcacactga cgttcggtca
    17161 gcggtctggc cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact
    17221 cttccaccat ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gcaaggattt
    17281 gcgtgtcaat caggggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt
    17341 cactgcctgc atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg
    17401 accagtcggt gatcccgaga ggatcaatta gcgccagatg ctcaccatca aaaagttgaa
    17461 tcaaccccag ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg
    17521 ccggaaaggc acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt
    17581 ggtaattcaa attgtattct ctttagtggg cgtcaaaaaa aacgccggat taaccggcgt
    17641 ctgacgactg acttaacgct caggctttat tgtccacttt gccgcgcgct tcgtcacgta
    17701 attctcgtcg caaaattttt ccgacgttag atttcggtaa ctcatcacga aactccacca
    17761 gcttcggtac tttgtagccc gtgagctgac ggcggcaaaa ggtcaccagt gactcttcgg
    17821 taagcgatgg atcttttttc actacaaaga ttttcaccgc ttcaccactg gagccggaag
    17881 gtacgccaac agccgcgact tcctgtacgc caggatgctg catgacgaca tcttcaatct
    17941 cgttgggata gacgttaaaa ccggaaacca gaatcatgtc ttttttacga tcgacaatgc
    18001 gcaggaaccc ttcttcatcc atcaccgcga tgtcgccggt gtgtaaccag ccatttttga
    18061 tgatctcatc ggtagcatcc ggacgctgcc agtaacccag catcacctgc ggtcctttga
    18121 cacaaagctc acccggttga cccggtggta cttcattatc atcatcatcc accagtttgg
    18181 cttccgtcga cggcaccggc aaaccgatgc taccactatg ataatcaata tcatatgggt
    18241 taacgctgac cagcggcgca cactcggtaa ggccatagcc ttccagcaga tactgtcctg
    18301 tcagtttcac ccaacgctct gccaccactt gctgcactgg catcccaccg cctgcggaaa
    18361 gatgcagact ggagaaatcc agctgctgga actctttatt gttcagcaac gcattgaaca
    18421 aggtgttaac gccagtgata gcggtaaacg gatatttcgc taactctttt accaaccctg
    18481 gaatatcacg cgggttagtg ataagcaggt tctgcccacc cagttcgata aacagcaggc
    18541 agttaatggt cagggcaaaa atgtgataca gcggcagtgc cgttactacc agttctttac
    18601 ctggatgcag caacggccca taggtggcgt tgacctgttc caggttcgcc agcatattgc
    18661 ggtgagtcag catcgcgcct ttcgccacac ctgtggtgcc gccggtgtat tgcaggaagg
    18721 ctaaatcttc cggcaccagt tcgggtttga cgtactgcat ccggtagccg ttatgcaggg
    18781 cgctacgaaa tgaaatggca tctggcagat ggtatttcgg caccaaacgc ttgatgtatt
    18841 taacaacgaa attgactacc gtgccttttg ccgtagatag ctgatccccc atacgggtca
    18901 gaattacgtg ctgaacggcg gttttatcaa ccactttttc cagtgtgtga gcaaagttag
    18961 acacaataac aatcgccgat gcgccgctat cgttaagctg atgctcaagc tcacgcgggg
    19021 tatacaacgg gttaacgttt acgacgatca tcccggcacg caaaatgcca aacagcgcca
    19081 ccggatattg caataaatta ggcatcatca acgcaacgcg atcgcctttc ttcagcccca
    19141 acccttgttg caaataagcg gcaaacgcgc gactgcgttc ttccagcttg cggaaggtca
    19201 tcacctcccc catattcaca aacgcaggct gatcggcgta gcgcgcgacc gactgctcaa
    19261 acatatctac cagagattga taacggtcag ggttgatctc cgtcggaacg tccgcgggat
    19321 aacggttaag ccaaaccttc ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg
    19381 caaccccaaa tgatatacat gccgttaaca taatattaac tcatcatacc agcttgataa
    19441 ttacccaacg aaaaggttgc gaagcgcgtc actatttatt tttatcttta ccgtaagaat
    19501 gcagaaacag cggaccagcc gctgtttctt tttctttaaa aacaagcaat tattcagtta
    19561 caactgtttg tactctcgcg gggccgggat tataccagcc ccatccgccg tagccatagg
    19621 gccagccacg accgccataa aaccatggat caatcggctg aggcggcata atcacctgct
    19681 gggttaaatg ccaacgtttg taacccgtta cttgcatcac cataaattta tagggcgtat
    19741 tgccgatttt gccgtcaacc gcaccagtga ttggcccgac taccgtaacc agttgtccac
    19801 ggaaatccac cgggtccaga aaaccgttca catcggcata aatgcgaccg cgagaaggtt
    19861 ctcccagcgt cggtctggct ccgctgtcca gcggtacggt agcaatttcc aggcgggttt
    19921 tcccttgctg gttttgtacc gcaaccactt tgccaccaaa gcgtgcctcc tgaccaacgt
    19981 acagctgcgg cgcactcatc acccgaacta aatcttgttg cggcgtggga ctactgcctt
    20041 taatggcgtc cggcacagtg acacaaccgc tcagcattag cgcaaacgta cctgccagta
    20101 tgcctttgat aacattcttt tgaaccgcca tgatgcgact cctttttttc tcagggcata
    20161 ctcttaagat tcattctttg cccggaagtt tcttccatgc gacgttgtta cgtaaataaa
    20221 ccggttcggc atgttccacc gccaccgttt taccctcggc aaacatctga caagcaatcg
    20281 gcagcatatc ttctgcagcg ggcagtaaaa cttctccgtc gcgcaaaacc agtccgctct
    20341 ctttaccgag atccggccag gcttgccagc ctgtacctac cgtcacccat tccccggaaa
    20401 gctgttgcat ccgttcatga acgagttcgg gtttgagtac ggcttcggtt tcttcgccgt
    20461 gccagacgcc gttttcatca cgctgatatt cggcccagta aacttcgccc atccgcgcat
    20521 caatggcagc cagcacgcgc gttgcgccgt ttttgcgcca cgccccctgc gccatcgtca
    20581 tgagcgtgga gacgccaatc atcggcagcg ccgcgccaag tgccagcccc tgcgcaatgc
    20641 ctataccaat gcgcacgccg gtaaaaccac cggggccgcg cccgtaggcc agagcatcaa
    20701 tatcagagag ggaggtaccg ctggtggtca ggatatcctg taccatcggt aagattcgtt
    20761 gagtatgttc acgagggcaa agctcaaaat gagcgttgac agtaccgtcg ttccacaggg
    20821 cgacagagca ggcctctgtc gcggtatcga tagccagaat tcgcatgggt cttcgtgctt
    20881 agatcaataa aaaggcgcgc atcataccat actccgtaac aaattacctg gaggatggta
    20941 tcgcaaggaa acgaaccgca cgggcaatgt cacgggtgcg cggcgcgggc ggcagactgg
    21001 cgagaaacgt cgcaccgtaa gggcgcatca caagccgatt atcgcaaatc accagcacgc
    21061 cgcgatcatc ggcgtcgcga atcagtcgcc ctaccccctg cttgagagta atcaccgcat
    21121 ccggcagttg tacttcatcg aacgggtcgc caccgcgcaa acggcaatct tccatgcgcg
    21181 cttttaacag tggatcatca ggagaggtaa acggcaattt gtcgataatc accaatgaca
    21241 gcgtatcgcc acgcacgtcc accccttccc agaaactgct ggttgccaca agaagcgcgt
    21301 taccggcgct gacaaactgc tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa
    21361 cgggaagcgt catggtagcg cggaactgct cggccagatc gcgcatcatg gcgtgcgagg
    21421 tacaaagcat aaaacaacga ccgttgttag cttcgatgat cggtcgcagc atagccgcca
    21481 gtttccgcgc ggaacccggt tgattggttt gcggcaggtt gcgcggcaca cagagtaacg
    21541 cctggcggct gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa
    21601 gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc
    21661 agctaccggg cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga
    21721 gagccagagt aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga
    21781 tctcttttag ccgcttcagc cgtgtgcgat acaacgtggc gcgctcaaat gccgcatcca
    21841 gcaaggcaga acgccccagt gacagtttcg ccacgtcata acaaagttcc agggtgtcat
    21901 cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc
    21961 gataacctgg ttcaccgagt tgcagacgaa aatcctgcgc actctgagca agacgatctg
    22021 cgcacttttg taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt
    22081 ttgccaggtc gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt
    22141 ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc ttccgggatc aactcgccaa
    22201 atccactctc tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat
    22261 cggcgtccat cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt
    22321 cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg ccacgcctgc gaatcttccg
    22381 ccacgctgac gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca
    22441 ggatcacatc gcttaatatt tgtaccggaa gatcgccccc cgccagcgcc tgctgttcga
    22501 gacgttcgag gcagaggtag tttgagcgcc ctttcagcaa cgccacgttg cccgtgtatt
    22561 tcaacgcctt tgagaccgtc ggcaaatcgc ggctatagag ctgatcctgc aacgcttttg
    22621 agccggtcga gataatgact ttctttttcg cccgcagcgc aggggccagg taagcgtagg
    22681 ttttgcccgt accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg
    22741 tgacggctac cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg
    22801 ccagctgacc gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacaggg
    22861 attatgtcag gatgagggcg ctttcgccag ttgaagtggt gacggcgacc tcacattgtg
    22921 gcagtctttg cagcacgaaa atggaaggat aatgaggaca aaatgatgac tatcgttcgt
    22981 atcgatgctg aagcccgctg gtctgatgta gtaatccaca acaacacgct ctactacact
    23041 ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa ccgccaacac gctggcgcag
    23101 attgacgccg tgctggaaaa acagggcagc aataaatcga gtattctgga tgccaccatt
    23161 ttcctggccg ataaaaacga cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc
    23221 gcgggtcatg cgccggtgcg ctgcacggta caagcgggtt tgatgaaccc gaagtataaa
    23281 gttgaaatta agattgtggc tgcggtgtaa gctttatcga agcaaaataa gtcagacgat
    23341 aatttatcga taatactggt cggttttaca taaatcgacc agagaataag atattactgt
    23401 tgatgcttct gctaattcat tatttatttt tatagtttca attttatcta taaaactaac
    23461 tatataaata aacataacca tgccgttgtg ttcgcccttt gattagcaac tctggttttc
    23521 tttcttagct gaacaatctg gttatagcgt gcggactgac cgttaatttt ctccatctta
    23581 ctggataaca caccatgagc tgctaccttt aaaacacctg aatgtcaaca ggttaactcg
    23641 tgccatatcg ttataatccc tttgatatta gatgcaaatt aaggtcatat atagccttat
    23701 cgataacatg gttaatttta aggataagaa tatgcctgct gtaatagata aagccctgga
    23761 tttcattggt gccatggatg tatcagcgcc aacaccaagt tcgatgaatg aaagcacggc
    23821 gaagggaatc tttaaatatt taaaagaact gggagtaccc gcgagtgccg ctgatattac
    23881 ggcgcgagct gaccaggaag gctggaaccc tgggttcaca gaaaaaatgg ttggatgggc
    23941 aaaaaaaatg gagacaggtg aacgctctgt gattaaaaat cctgagtact tttcaacata
    24001 tatgcaggaa gaactaaaag cactggtctg agttaaattt atatcagcat aaatgggtca
    24061 ggacgctttt aatcacatat aaaaagcgtc ctttttctca caatcaaata atcatcggct
    24121 tctataacga aatcgatacg gattagtgtg ttactcgtct tcatcctcaa aacgcgccac
    24181 gattcgctcg ccggagtggt tggcacgcag ctcttctgcc accagcgcga ttgcctggcc
    24241 gctgctcatc ccttgtgcca tcagttcctg gatccgctcg acagcttttt gctgctgttc
    24301 atgggtgagt gaaggtaaac ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa
    24361 ttatttcatg ctacccggca catagccagt agagtcagga ctgatgaaga cgttatctcc
    24421 cgctgtgatt actttaccct ggcgtcagga cgccgctgaa ttttatttct cccgcttaag
    24481 ccacctgccg tgggcgatgc ttttacactc cggctatgcc gatcatccgt atagccgctt
    24541 tgatattgtg gtcgccgatc cgatttgcac tttaaccact ttaggtaaag aaaccgttgt
    24601 tagtgaaagc gaaaaacgca caacgaccac tgatgacccg ctacaggtgc tccagcaggt
    24661 gctggatcgc gcagacattc gcccaacgca taacgaagat ttgccatttc agggcggcgc
    24721 actggggttg tttggctacg atctgggccg ccgttttgag tcactgccag aaattgcgga
    24781 acaagatatc gttctgccgg atatggcagt gggtatctac gattgggcgc tcattgtcga
    24841 ccaccagcgt catacagttt ctttgctgag tcataatgat gtcaatgccc gtcgggcctg
    24901 gctggaaagc cagcaattct cgccgcagga agatttcacg ctcacttccg actggcaatc
    24961 caatatgacc cgcgagcagt acggcgaaaa atttcgccag gtacaggaat atctgcacag
    25021 cggtgattgc tatcaggtga atctcgccca gcgttttcat gcgacctatt ctggcgatga
    25081 atggcaggca ttccttcagc ttaatcaggc caaccgcgcg ccatttagcg cttttttacg
    25141 tcttgaacag ggtgcaattt taagcctttc gccagagcgg tttatccttt gtgataatag
    25201 tgaaatccag acccgaccga ttaaaggcac gctaccacgc ctgcccgatc ctcaggaaga
    25261 tagcaaacaa gcagaaaaac tggcgaactc agcgaaagat cgtgccgaaa atctgatgat
    25321 tgtcgattta atgcgtaatg atatcggtcg tgttgccgta gcaggttcgg taaaagtacc
    25381 agagctgttc gtggtggaac ccttccctgc cgtgcatcat ctggtcagca ccataacggc
    25441 gcaactacca gaacagttac acgccagcga tctgctgcgc gcagcttttc ctggtggctc
    25501 aataaccggg gctccgaaag tacgggctat ggaaattatc gacgaactgg aaccgcagcg
    25561 acgtaatgcc tggtgcggca gcattggcta tttgagcttt tgcggcaaca tggataccag
    25621 cattactatc cgcactctga ctgccattaa cggacaaata tactgctctg cgggcggtgg
    25681 aattgtcgcc gatagccagg aagaagcgga atatcaggaa acttttgata aagttaataa
    25741 gatattacgc caactggaga agtaagacgt ggaataccgt agcctgacgc ttgatgattt
    25801 tttatcgcgc tttcaacttt tgcgcccgca aattaaccgg gaaaccctaa atcatcgtca
    25861 ggctgccgtg ttaatcccca tcgtccgtcg accgcaaccg gggttgttgc tgactcagcg
    25921 ttcaattcat ctgcgtaaac acgctggaca agtggcattc cctggaggtg cagtcgatga
    25981 cacggacgca tcagttatcg ccgccgcgct gcgcgaagct gaagaagagg ttgctatacc
    26041 gccttccgcc gttgaagtta tcggcgtgct gccgcccgtc gatagcgtaa ctggctacca
    26101 ggtaacccca gtggtcggca ttatcccgcc cgatctgccg tatcgcgcca gtgaagatga
    26161 agtctcggcg gtgtttgaaa tgccgctcgc ccaggcatta catctgggtc gttatcaccc
    26221 tttagatatc taccgccgtg gcgattcaca tcgggtatgg ctgtcctggt acgaacagta
    26281 ttttgtatgg ggaatgaccg caggcataat tcgtgagctg gcgctgcaaa ttggtgtgaa
    26341 accctgacta tacttatctt tacatctaca aaacactact tgagacaatc atcgcaatat
    26401 tagttaaatc gcggtttttg attagtttaa ttcatgtgaa tagttaagcc agtcgccgcg
    26461 ttccctctta cactatgcgc tgttattagt tcgttactgg aagtccagtc accttgtcag
    26521 gagtattatc gtgattagtc tattcgacat gtttaaggtg gggattggtc cctcatcttc
    26581 ccataccgta gggcctatga aggcaggtaa acagttcgtc gatgatctgg tcgaaaaagg
    26641 cttactggat agcgttactc gcgttgccgt ggacgtttat ggttcactgt cgctgacggg
    26701 taaaggccac cacaccgata tcgccattat tatgggtctt gcaggtaacg aacctgccac
    26761 cgtggatatc gacagtattc ccggttttat tcgcgacgta gaagagcgcg aacgtctgct
    26821 gctggcacag ggacggcatg aagtggattt cccgcgcgac aacgggatgc gttttcataa
    26881 cggcaacctg ccgctgcatg aaaacggtat gcaaatccac gcctataacg gcgatgaagt
    26941 cgtctacagc aaaacttatt attccatcgg cggcggtttt atcgtcgatg aagaacactt
    27001 tggtcaggat gctgccaacg aagtgagcgt gccgtatccg ttcaaatctg ccaccgaact
    27061 gctcgcgtac tgtaatgaaa ccggctattc gctgtctggt ctcgctatgc agaacgaact
    27121 ggcgctgcac agcaagaaag agatcgacga gtatttcgcg catgtctggc aaaccatgca
    27181 ggcatgtatc gatcgcggga tgaacaccga aggtgtactg ccaggcccgc tgcgcgtgcc
    27241 gcgtcgtgcg tctgccctgc gccggatgct ggtttccagc gataaactgt ctaacgatcc
    27301 gatgaatgtc attgactggg taaacatgtt tgcgctggca gttaacgaag aaaacgccgc
    27361 cggtggtcgt gtggtaactg cgccaaccaa cggtgcctgc ggtatcgttc cggcagtgct
    27421 ggcttactat gaccacttta ttgaatcggt cagcccggac atctataccc gttactttat
    27481 ggcagcgggc gcgattggtg cattgtataa aatgaacgcc tctatttccg gtgcggaagt
    27541 tggttgccag ggcgaagtgg gtgttgcctg ttcaatggct gctgcgggtc ttgcagaact
    27601 gctgggcggt agcccggaac aggtttgcgt ggcggcggaa attggcatgg aacacaacct
    27661 tggtttaacc tgcgacccgg ttgcagggca ggttcaggtg ccgtgcattg agcgtaatgc
    27721 cattgcatct gtgaaggcga ttaacgccgc acggatggct ctgcgccgca ccagtgcacc
    27781 gcgcgtctcg ctggataagg tcatcgaaac gatgtacgaa accggtaagg acatgaacgc
    27841 caaataccgc gaaacctcac gcggtggtct ggcaatcaaa gtccagtgtg actaatactt
    27901 cttactcgcc catctgcaac ggatgggcga atttatcccc gctttctcgt ctgctgtaat
    27961 attccccact acacttccac tgttgcgtca ggcgtttgtc gccatacgct tacagggtgg
    28021 cccgcatgca aaaagcacaa cggatcatta aaacctatcg ccgtaatcga atgattgttt
    28081 gtacgatttg cgcactcgtt acgctcgctt cgaccctgag cgtgcgattt atttcacagc
    28141 gtaacttaaa tcaacaacgg gtagtacaat tcgccaatca tgctgtagag gaattagata
    28201 aagtactgct tcccctacag gcaggtagcg aagtcttgct tccgctgatt ggtctgccct
    28261 gctctgtcgc ccatttgcca ttacgtaaac aggcggcaaa actccaaact gtgcgatcca
    28321 ttggcctggt gcaagacggc acactctatt gctccagcat ttttggttat cgcaatgtgc
    28381 ccgtcgtgga cattctggct gaacttcctg caccgcaacc acttttacgc ctgacgatcg
    28441 accgtgccct gattaaaggc agtccggttt tgattcaatg gacgcctgca gcgggcagta
    28501 gcaatgctgg ggtcatggag atgattaaca tcgacttact gacggcaatg ctccttgagc
    28561 cacaactgcc gcaaatcagt agcgccagcc tgacggtgga caaacggcat ttgctctatg
    28621 gtaatgggct ggtagattcc cttccgcaac ctgaagacaa tgaaaactat caggtttctt
    28681 cgcaacgctt tccttttacc attaacgtta atggtccggg ggctacggcg ctggcatggc
    28741 actatcttcc aacacaatta ccgctggcgg tgctgctaag tttactggtg ggctacatcg
    28801 cctggctggc gaccgcttac cggatgagct tttcccgcga aatcaatctg ggcctggcgc
    28861 aacatgagtt cgaattgttc tgtcagcctt tgcttaatgc gcgcagccag caatgtattg
    28921 gtgtagagat tttgctgcgc tggaacaatc cgcgtcaggg ctggatttca ccggatgtgt
    28981 ttattcctat cgcggaagaa catcatttaa ttgtgccact gacccgctat gtgatggcag
    29041 aaaccattcg tcagcgccat gttttcccga tgagtagtca gtttcatgtt ggcattaacg
    29101 tcgcacccag ccattttcgc cgtggtgtgc tgataaaaga tctcaatcag tactggttta
    29161 gcgctcaccc gattcagcaa ctgatcctcg aaatcaccga acgcgatgcc ttactggatg
    29221 ttgattatcg gattgcccgc gagctgcatc gtaaaaatgt caaactggcg attgatgact
    29281 tcggcaccgg caacagttca ttttcctggc ttgaaacatt acgtcctgac gtgctgaaaa
    29341 ttgataagtc atttaccgca gctataggtt ctgacgcggt taactcgacg gtgaccgata
    29401 tcatcatcgc gctggggcaa agactgaata ttgaactggt ggcggagggc gtggaaacgc
    29461 aagaacaagc gaagtatttg cgccgtcatg gcgtgcatat tttgcaaggg tatttgtacg
    29521 cacagccgat gccgctacgt gattttccca aatggctggc gggcagccaa ccgccgcccg
    29581 cccggcataa tggacatatc acgcccatta tgccattacg ttaacgctta ctcatcttca
    29641 tcgtgcgccg gttgctcttt aacaatgcga accagatcaa cacgataatc attggcttca
    29701 atgatggtga tatgcagtgg ccctacatca atcacatccc ccacacgggg aatgtgacca
    29761 tttgccgaga tcacgaggcc cgcgaccgtc gcgatatcat cgtcatcggc aaggtgctca
    29821 acatcaagcg cctgctgcaa ggcatgcaaa tctgtaccgc cttttaccag ccagccgtca
    29881 ccatcagtaa tgatttccgg cgtttcgtca gcgtccggga attcacccgc aatggcttcc
    29941 agcacatcca gcggcgtgac caagccttgt accacaccaa actcgttggt cacgataaca
    30001 aagctcccgc gagcacgacg cagcacgccc agcaggttga tcggatcgag ggtttcaggg
    30061 acgataatcg ccggagacgc cgaagcaatc gccgccacat caacgccctc ttccagcgcc
    30121 accagcagtt ctttagcacg tacgattccg atgatttcat ccagttcacc gcgacatacc
    30181 gggaacaggc tgtgcggtga ggagagcagc tgctcgcgga tttcatcgac cccgagatta
    30241 gcgtcaaccc agcttatttc accgcgcggc gtcatgatcc cgcgcagaga acgcgacgcc
    30301 agcgtcagta cgccgttaat catgtaacgt tcttcttcgg caaatgcacc ttccgggatc
    30361 ggcatcggca tcgggttatc ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc
    30421 aaacgcagga tggcatcggc agtacgcgct cgcagcggca aagtcgactg gtgacgaata
    30481 aagttgcgac gcgcaatctg gttaaacact tcgatgatga tcgagaagcc aatcgcggca
    30541 tacaggtaac ctttcggaat gtggaaaccg aaaccttctg ccaccagact cagaccaatc
    30601 attaacagga agctcagaca gagcaccacc accgtggggt gctggttaac aaatcgcgtc
    30661 agcggtttgg atgccagcaa cataaccgcc atcgcaatca ctaccgccgc catcatcacc
    30721 ggcagatggt taaccatccc tacagcagta attaccgcat ccaacgagaa gacggcgtca
    30781 aggatgacaa tctgtgtgac gactacccag aaactggcgt agcctttacc gtggccggaa
    30841 tcatgatcgc ggttttccag ccgttcatgc agttcggttg ttgctttgaa cagcaagaat
    30901 atccccccga acaacataat caggtcgcgt ccggagaagg agaaatccat gacggtaaat
    30961 agcggtttgg tcagcgtgac catccatgaa atcagcgaca gcagccccag acgcataatc
    31021 agcgccagtg ataaccccag caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca
    31081 gcaagaatgg cgatgaagac caggttatcg atacccagca caatttcgag aacaacaagc
    31141 gtgagtagcc ccgcccaaat tgaggggtcc attaagaatt ccatgacaag ctcctgctta
    31201 aggaatagct attcgacgcc agaaataatg caggcgtaac gacaaaatgc aaacgaaagg
    31261 tgcggcatag agtgccagaa aggcaggcgt taaaaggcct gatgctgaaa tgacgtcggt
    31321 gacgatccat actgcgggct actgccctat actccatggt tgttaaacgg gagttaaaca
    31381 tatcagagac gcctctgatt tggcaaagat ttaccttcct ttgcaaacga atgtgacaag
    31441 gatattttac ctttcgaaat ttctgctaat cgaaagttaa attacggatc ttcatcacat
    31501 aaaataattt tttcgatatc taaaataaat cgcgaaacgc aggggttttt ggttgtagcc
    31561 cttatctgaa tcgattcgat tgtggacgac gattcaaaaa tacatctggc acgttgaggt
    31621 gttaacgata ataaaggagg tagcaagtga ccattgctat tgttataggc acacatggtt
    31681 gggctgcaga gcagttgctt aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg
    31741 gctggatcga tttcgttcca ggtgaaaatg ccgaaacgct gattgaaaag tacaacgctc
    31801 agttggcaaa actcgacacc actaaaggcg tgctgtttct cgttgataca tggggaggca
    31861 gcccgttcaa tgctgccagc cgcattgtcg tcgacaaaga gcattatgaa gtcattgcag
    31921 gcgttaacat tccaatgctc gtggaaacgt taatggcccg tgatgatgac ccaagctttg
    31981 atgaactggt tgcgctggca gtagaaacag gccgtgaagg cgtgaaagcg ctgaaagcca
    32041 aaccggttga aaaagccgcg ccagcacccg ctgccgcagc accaaaagcg gctccaactc
    32101 cggcaaaacc aatggggcca aacgactaca tggttattgg ccttgcgcgt atcgacgacc
    32161 gtctgattca cggtcaggtc gccacccgct ggaccaaaga aaccaatgtc tcccgtatta
    32221 ttgttgttag tgatgaagtg gctgcggata ccgttcgtaa gacactgctc acccaggttg
    32281 cacctccggg cgtaacagca cacgtagttg atgttgccaa aatgattcgc gtctacaaca
    32341 acccgaaata tgctggcgaa cgtgtaatgc tgttatttac caacccaaca gatgtagagc
    32401 gtctcgttga aggcggcgta aaaatcacct ctgttaacgt cggtggtatg gcattccgtc
    32461 agggtaaaac ccaggtgaat aacgcggttt cggttgatga aaaagatatc gaggcgttca
    32521 aaaaactgaa tgcacgcggt attgagctgg aagtccgtaa ggtttccacc gatccgaaac
    32581 tgaaaatgat ggatctgatc agcaaaatcg ataagtaacg tattgtgttg attatcactc
    32641 agttttcaca ctttagtctt acgtaaacag gagaagtaca atggagatta ccactcttca
    32701 aattgtgctg gtatttatcg tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt
    32761 tcagtttcac cgtccgctaa tcgcgtgtac cctggtgggt atcgttcttg gggatatgaa
    32821 aaccggtatt attatcggtg gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg
    32881 tgctgcagtt gcgcctgacg ccgctctggc atctatcatt tctaccattc tggttatcgc
    32941 aggtcatcag agcattggtg caggtattgc actggcaatc cctctggccg ctgcgggcca
    33001 ggtactgacc atcatcgttc gtactattac cgttgctttc cagcacgctg cggataaggc
    33061 tgctgataac ggcaacctga cagcgatttc ctggatccac gtttcttctc tgttcctgca
    33121 agcaatgcgt gtggctattc cggccgtcat cgttgcgctg tctgttggta ccagcgaagt
    33181 acagaacatg ctgaatgcga ttccggaagt ggtgaccaat ggtctgaata tcgccggtgg
    33241 catgatcgtg gtggttggtt atgcgatggt tatcaacatg atgcgtgctg gctacctgat
    33301 gccgttcttc tacctcggct tcgtaaccgc agcattcacc aactttaacc tggttgctct
    33361 gggtgtgatc ggtactgtta tggcagtgct ctacatccaa cttagcccga aatacaaccg
    33421 cgtagccggt gcgcctgctc aggctgctgg taacaacgat ctcgataacg aactggacta
    33481 acaggtgagc gaaatggttg atacaactca aactaccacc gagaaaaaac tcactcaaag
    33541 tgatattcgt ggcgtcttcc tgcgttctaa cctcttccag ggttcatgga acttcgaacg
    33601 tatgcaggca ctgggtttct gcttctctat ggtaccggca attcgtcgcc tctaccctga
    33661 gaacaacgaa gctcgtaaac aagctattcg ccgtcacctg gagttcttta acacccagcc
    33721 gttcgtggct gcgccgattc tcggcgtaac cctggcgctg gaagaacagc gtgctaatgg
    33781 cgcagagatc gatgacggtg ctatcaacgg tatcaaagtc ggtttgatgg ggccgctggc
    33841 tggtgtaggc gacccgatct tctggggaac cgtacgtccg gtatttgcag cactgggtgc
    33901 cggtatcgcg atgagcggca gcctgttagg tccgctgctg ttcttcatcc tgtttaacct
    33961 ggtgcgtctg gcaacccgtt actacggcgt agcgtatggt tactccaaag gtatcgatat
    34021 cgttaaagat atgggtggtg gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg
    34081 cctgtttgtc atgggggcat tggttaacaa gtggacacat gtcaacatcc cgctggttgt
    34141 ctctcgcatt actgaccaga cgggcaaaga acacgttact actgtccaga ctattctgga
    34201 ccagttaatg ccaggcctgg taccactgct gctgaccttt gcttgtatgt ggctactgcg
    34261 caaaaaagtt aacccgctgt ggatcatcgt tggcttcttc gtcatcggta tcgctggtta
    34321 cgcttgcggc ctgctgggac tgtaagactg ttgtacacta ccggggcctt ttggccccgt
    34381 ttttttatct ggaggattaa tgacaatcac ggacctggta ctgattcttt tcatcgccgc
    34441 actcctggcc ttcgcgatct acgatcagtt catcatgccc cgccgtaacg gccccaccct
    34501 gctggcaatt cctttgctcc ggcgtggtcg catcgatagc gttatcttcg tcggattgat
    34561 tgtcattctt atctataaca acgtcacgaa tcatggtgcg ttaataacga catggttatt
    34621 aagcgcactg gctctgatgg gtttttatat attctggatc cgcgttccga agatcatctt
    34681 taaacaaaaa ggttttttct tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat
    34741 gaacttgtcg gaagatggcg tgttggtgat gcaattagaa cagcgtcggc tgttaatccg
    34801 cgttcgaaat atcgacgatc tggaaaaaat ttataagctt ctcgtatcaa ctcaataact
    34861 tatgaattta gccaaagcta tgtttagtgt atttttaata atcagacata gcttaggcta
    34921 tattatctct tcccttattt gttatttata ttaacgtttc attgatatat aaatctaaat
    34981 gaaaatcgtt atcaataaag caatgaaata atatattcca acagttgttt tatattctca
    35041 aaatatgtta aggttgcgcc ctcatttggg gagtagccga tttccagatt ccggaaatgt
    35101 acgtgtcaac atactcgttg caaaacgtgg cacgtacgga ctgaagactt tcagtcaggc
    35161 gagaccatat gcacatcaat cgctatgcct gcatgaacgc gaaaacccat cgcggcttgt
    35221 tgttcatgta tgcatgtttg ctgggggcga tgatgtgttt tatggatacc ccggtcagga
    35281 cgttgtcatg aatatcactg ctactgttct tcttgcgttt ggtatgtcga tggatgcatt
    35341 tgctgcatca atcggtaaag gtgccaccct ccataaaccg aaattttctg aagcattgcg
    35401 aaccggcctt atttttggtg ccgtcgaaac cctgacgccg ctgatcggct ggggaatggg
    35461 catgttagcc agccggtttg tccttgaatg gaatcactgg attgcgtttg tgctgctgat
    35521 attcctcggc gggcgaatga ttattgaggg ttttcgtggc gcagatgatg aagatgaaga
    35581 gccgcgccgt cggcacggtt tctggctact ggtaaccacc gcgattgcca ccagcctgga
    35641 tgccatggct gtgggtgttg gtcttgcttt ccttcaggtc aacattatcg cgaccgcatt
    35701 ggccattggt tgtgcaacct tgattatgtc gacattaggg atgatggttg gtcgctttat
    35761 cggctcaatt attgggaaaa aagcggaaat tctcggcggg ctggtgctga tcggcatcgg
    35821 cgtccagatc ctctggacgc acttccacgg ttaataagaa cgctgccaga ggtgaatatt
    35881 aaagtccgtc tggcagtcga acacttcttt tgctgccagc gtttgccaga cttctggctt
    35941 cgcacgccag gcaaacggcg tcatctgcaa taatgcgacg gcttcatcac cgcgaagacg
    36001 catcggataa cacaactcat cactctgctg taatgtaaaa ccttccagtt gttctgcatg
    36061 aggtgcatga agatgtactt cattgtaaat cagccccttc agctccatca aatgtcgcgg
    36121 tcccggcgtg gcagtaatga cccaaccgcc gggcttcact actcgcgcta attcttctgc
    36181 tttacacggc gcgtaaatac gtattatggc gtccatactg gtatcggaaa acggcaaacg
    36241 gtggctggaa gcgacacaaa aagtgacctg cggatagcgt ttcgccgccg cttttatcgc
    36301 taccttcgag acatccaggc caaaggtggt gatttcgggc aacgcatcgg caaatgcgtg
    36361 tgtgtaatac ccttcaccac agccaatatc cagcaccgcc gtggccttat catcaagccg
    36421 ttcccgcagt tgggcgacaa ttgcatcacg cagcggctga taatgtccgg catctaagaa
    36481 tgcgcggcgt gcttgcatca tttccgcgct gtcgcccgga tcacgagacc gtttatgctg
    36541 aacgggcagc agattgacat acccttcttt cgccatatca aactgatgtc gttggggaca
    36601 gatatagctg tttttttcac gcgtaagagg ctgatggcaa agtggacagg aaaacgacat
    36661 gacaactccg gcaggatact aaaggccgca agtgtaacgc gaatcacgcg gcgggcaaat
    36721 atccgaacgc caggagcgaa gataaagcat agtaaaaagc ctcgcattcg acgaggcttt
    36781 atagcgagat tgaagcgtat tcacacttca gatcagtgga ttcgatcaga tagctgttac
    36841 gttaacagct gccggacctt tctggccgtc ctgaatttcg aactcaacgt tctgaccttc
    36901 agccagagtt ttgaagccat taccctggat agcggagaag tgtacgaaca cgtctttgct
    36961 gccatcagca ggagtaatga agccaaaacc tttagactcg ttgaaccact taacctgacc
    37021 tttaatcttt gccatttgaa aaattcctta gattgttttc ttcgcccgca ggcataacat
    37081 agataaaact gacacattac tgcatgaggc accaatataa ggctcggcag agaagcggta
    37141 ttcaacgtca acgtgtttac tcaggacttc tttactgaaa atgccacaca taaacagaac
    37201 tgtacctcgt ttaacccgaa atctgttatc acatacaacg ttaattatgg caagccattt
    37261 ttaaacatgt ctcgatcaga cgcacaaatc tcgatacgtc ttccactttt tttgcacact
    37321 tatgcaacgg aatacgcgcc gatatatcat tgtgcttaac cttgccagtt caggcagata
    37381 cttaaactgg cgtagtttct aacatagttc aataagttca gttgttccag gccagtggga
    37441 gaagttatta catagtgcgt gcaatatcac attttctgct atgcaatgaa taaaaagtta
    37501 tatcactttt tctcataaaa cagtcagtta acggctatca attaccctaa agagaagtca
    37561 atccccaaaa ggattgtaaa tttaaaataa gaaaaattga tgagtgagca aaaaaaatca
    37621 agagagaaac g
//
LOCUS       DAZGBC010000027        37429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGBC010000027 DAZGBC010000000
VERSION     DAZGBC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.027"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..326
                     /gene="ytfI"
                     /locus_tag="ACHK1J_001404"
     CDS             <1..326
                     /gene="ytfI"
                     /locus_tag="ACHK1J_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO7910704.1"
                     /translation="IDDDFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNF
                     LVNAGCQQVELAEQVDAIVSANLAADSDITKEKICEKYKSTIEAFKKEQLALPVLVRR
                     KNSET"
     gene            complement(389..943)
                     /gene="ytfJ"
                     /locus_tag="ACHK1J_001405"
     CDS             complement(389..943)
                     /gene="ytfJ"
                     /locus_tag="ACHK1J_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7910705.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1268..1474
                     /gene="ytfK"
                     /locus_tag="ACHK1J_001406"
     CDS             1268..1474
                     /gene="ytfK"
                     /locus_tag="ACHK1J_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO7910706.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1536..2879)
                     /gene="paeA"
                     /locus_tag="ACHK1J_001407"
     CDS             complement(1536..2879)
                     /gene="paeA"
                     /locus_tag="ACHK1J_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO7910707.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3202..3840)
                     /gene="msrA"
                     /locus_tag="ACHK1J_001408"
     CDS             complement(3202..3840)
                     /gene="msrA"
                     /locus_tag="ACHK1J_001408"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO7910708.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4046..5779
                     /gene="tamA"
                     /locus_tag="ACHK1J_001409"
     CDS             4046..5779
                     /gene="tamA"
                     /locus_tag="ACHK1J_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO7910709.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5776..9555
                     /gene="tamB"
                     /locus_tag="ACHK1J_001410"
     CDS             5776..9555
                     /gene="tamB"
                     /locus_tag="ACHK1J_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO7910710.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRAALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMDVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDITKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLSAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9558..9899
                     /gene="ytfP"
                     /locus_tag="ACHK1J_001411"
     CDS             9558..9899
                     /gene="ytfP"
                     /locus_tag="ACHK1J_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO7910711.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(10182..10322)
                     /locus_tag="ACHK1J_001412"
     CDS             complement(10182..10322)
                     /locus_tag="ACHK1J_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7910712.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHSSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(10455..10757)
                     /locus_tag="ACHK1J_001413"
     CDS             complement(10455..10757)
                     /locus_tag="ACHK1J_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO7910713.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPHLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGISSKPVHYARVPFG"
     gene            complement(10801..11925)
                     /locus_tag="ACHK1J_001414"
     CDS             complement(10801..11925)
                     /locus_tag="ACHK1J_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO7910714.1"
                     /translation="MVLLNGNTVYSPLKELVKMPDFSVSLDREQDKNTLQELNNRVIS
                     LSMTDNRGFEADRVTLVLDDADGQLALPGRGTHIRVMLGWKGEPLVDKGTYIVDEVAH
                     EGPPDRLTLSANSADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISDMLMDIE
                     IDHADQTEESDISFLTRMADMLGAITTVKNGCLLFILPGGGVTADGRPLPSFALARSD
                     GDSHQFRIADRQAYTGVKAYWLDLNHGKKKAVNIKKRKTHAEKSSSREGSYMEGAEGN
                     VFVLRKTYPNEIAAQRAAAARWQQLQRGAAEFSITLARGRAELYPEMHGTVTGFKTEI
                     DNQDWLIAKVEHNIDGSGFTTRLELEARIPEWIAEKESNG"
     gene            12080..13267
                     /locus_tag="ACHK1J_001415"
     CDS             12080..13267
                     /locus_tag="ACHK1J_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO7910715.1"
                     /translation="MAETRFHGVRVRENADLVTAINDIDSSVIGIVAVADDADAKTFP
                     LDKPVLLTRVMDVLGKAGKTGTLYKSLKAIADQVSTRVVVVRVAAAKEGDGQPTQTQL
                     VIGEVKNDGTYTGMQALLVAEQNEAVGVRPRILAVPSLDTKEVVAALVTVAKKLRAFV
                     YAGCPGCNTVQEAVTWRADLAHREVMLIWPDFIAYNPNSGQNEAFPASAYACGLRAKI
                     DHEQGWHKSLSNVAVNNVLGISVPVFWSLQAEDSDAETLNDKEITTLIKRNGFRFWGN
                     RTLDTTTYTFETYTRTAQSLADTIAESQFETVDKPLTPSNVKDVLSDIRAKLSAFVTA
                     GKLIGAECWYDVVDNGTTDLRQGVVRIRYKYTPVPPLEDLELHQTFTDEFFAGAFASL
                     GGE"
     gene            13267..13776
                     /locus_tag="ACHK1J_001416"
     CDS             13267..13776
                     /locus_tag="ACHK1J_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO7910716.1"
                     /translation="MAVPNKLRVFTVYIDGENKLGRVTSFTPPKLTRKTESFRGAGMP
                     GSVSVDFGLDDGALDLSFAVGGADAHLFSRYAGSLDEVRLRFSGEYYSDADIRYVDIE
                     VRGRITEIDMGEAKQGEDTEHSYSMKNTWYRLSVDDLEKIEFDVLNFIWRIDGKDVLP
                     DRTRSMLGF"
     gene            13824..14213
                     /locus_tag="ACHK1J_001417"
     CDS             13824..14213
                     /locus_tag="ACHK1J_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910717.1"
                     /translation="MDKETEMTTDTAVSNGEASAVAVAATAAPKNVVKLTVPVVRGDV
                     SYSEIKIGPLANQAGSLRGLSLLALYNMNCDAMVTWLARVTEPKLKEAEMYAMNVADF
                     TALSSAAVNFFAPSGSNAPAPENTAEP"
     gene            14234..14371
                     /locus_tag="ACHK1J_001418"
     CDS             14234..14371
                     /locus_tag="ACHK1J_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO7910718.1"
                     /translation="MEDPVADIAVIFNWPPAEIFAMTPAEVVAWRERAAARSGSHNHD
                     E"
     gene            14361..17153
                     /locus_tag="ACHK1J_001419"
     CDS             14361..17153
                     /locus_tag="ACHK1J_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7910719.1"
                     /translation="MMNNLHIRVALAAADTLTQPLRRARETVGALSDSLRETQDSIRS
                     LERQSEAFAAAQTKFDEATRKIAAVQQQLNRLQQAEREGTVLSERQREMMASLTTRLE
                     RLNRVRDVHQRRLEDSRRELERHGIAAEGSSQTIQAAIRRTAEYNEELARQRQALAQV
                     TRAQQRYEQAQERLGRIRNAGAAALAAGAAGLYAGGRAVAPVMDLQRHGAVIAAQAGE
                     GASDAVGYTGVINHMVHTGITPDAQLAAEAVGAVRSTLGGLGAVSENELTRITRKMLD
                     MQAVMGGDIPQHIQAAAIMIKNGLARSSDEAVDLMVAGMQRMGPVMRDELPEILHEYS
                     THFRNMGLTGSETMTLLADMAQQGKFALDKTGDAIKEFSIRGSDMSKASAEAYALIGL
                     DAARMSSAVASGGTAARAALQKTASGLLQIVDPAERANAAIALFGTPVEDLSVDQIPA
                     FLGALARVSDRMADVSGVADRMGQTLRDNLSGDVARLTGTFSALRSGIIGEITDDLRG
                     LVRMATAWGERLQAWVNAHPELVRMIALTTGAVLALTTAMGALSVAAWMVVAPLLKLR
                     LGLALLRAIPAGLSVAGTLFGGLTGVISAVGAALGALTWPVVVAVGALVGGALLIWRY
                     WDRVRAFVSGFIEGVQSALAPFMATASRFAPLLDLVSGALSRILGWFKSLLTPVSSTR
                     DLLEQCTSAGQTFGSTLAGALATVFSPLKALIDGLAWVLEKLGVLPGEAERVKRKLDE
                     ARKTPVAWEWDPEQKKMVQKAWHWSPPGGASVPSGASGTQPPPVALPAGIAPDGNRNV
                     LRHLKGIEGNTGGLLAENRKRIGPGDIVFKHLPRALAVRGQWHEPVVAPSSGGMRALP
                     QEEIQVQQWPTARPAPRMHQQETFASAFQGEIHVHLHGVSSHNPRELARMVGDAVRAE
                     MNRMARAGTGSFRDRD"
     gene            17164..17655
                     /locus_tag="ACHK1J_001420"
     CDS             17164..17655
                     /locus_tag="ACHK1J_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7910720.1"
                     /translation="MMMIYGMFVFQLSTLPHQQVEQSKRWRHVSNERVNRSSSWQYIG
                     SGEDHITMSGVLCPEITGGEVSLTALNTLAYTGLPWPLIDGVGQIYGMYVITEIRTTR
                     QELDRYGMARKIEFTVSFQRCNEDLRERLQSGSVGDLMAGLKNSASSALSSASSALSG
                     LML"
     gene            complement(17666..18253)
                     /locus_tag="ACHK1J_001421"
     CDS             complement(17666..18253)
                     /locus_tag="ACHK1J_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO7910721.1"
                     /translation="MHTIKSARNAAYNRDGTIDCEIQFEGEESWLPYTATEYDNSPHG
                     KKLWGELKAGNHGPVSEYQENDTDRENARRLKNDEINAWRNAMEAASYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGILPLDFFWTDAQNNDVPVSADELIALSDAAAKALFAKGM
                     EIHIRQRNMKKEIAALNDTAAILDYKVGWGKEAEA"
     gene            complement(18265..18696)
                     /locus_tag="ACHK1J_001422"
                     /pseudo
     CDS             complement(18265..18696)
                     /locus_tag="ACHK1J_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(18991..19605)
                     /locus_tag="ACHK1J_001423"
                     /pseudo
     CDS             complement(18991..19605)
                     /locus_tag="ACHK1J_001423"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(19605..20228)
                     /locus_tag="ACHK1J_001424"
     CDS             complement(19605..20228)
                     /locus_tag="ACHK1J_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO7910722.1"
                     /translation="MNNRLLPPSSPSFLHHSEQGAQRLSELPIHINTLWNPDTCPAHL
                     LPWLAWALSVDRWDETWSEEARRQIIRDSWHIHRHKGTISAMRRAVEPFGAILSITEW
                     WENNDPPGSFRLDIDISERGLTEETRQEMERMITTVRPVSRHLAAMKMTYVARGISRI
                     TGATLTGETIVVYPAAELTSPLSVSGDIHPGGVTYEGITVTIRPDKE"
     gene            complement(20221..21117)
                     /locus_tag="ACHK1J_001425"
     CDS             complement(20221..21117)
                     /locus_tag="ACHK1J_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7910723.1"
                     /translation="MPTIDLSQLPRPQIIESLDYESILKDVKAFMIDAFPENMRSAVS
                     AAIALESEPLNIIAQAIAFREMLLRQRINDAAAGCMLSHAVSTDLDNLAAIVNAKRLE
                     LKPATDTEAAIMESDSAFRLRAQSGFEGFSVAGPSAAYEYFARSASGLVADARATSPS
                     PATVIVSILSTEGDGTASPELIATVSRALNDETVRPLADRLTVQSATIVSYDIEAVLY
                     FYPGPESEPILAAARNALDTWLKTQNAIGRDVARSAIMATLHVPGVQRVELKKPAQNV
                     VISNTQSARCTSRNISAGGTDE"
     gene            complement(21104..21469)
                     /locus_tag="ACHK1J_001426"
     CDS             complement(21104..21469)
                     /locus_tag="ACHK1J_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO7910724.1"
                     /translation="MTPVCTGMNPHGSGCISDTEHLRHSVNNILTTPRGSRIMRREYG
                     SLLPELLDAPQNDVTRLQCMSAVVMALAQWEPRIAINRVDIAWLPDGRALLAISGVLT
                     RTMQPVQMSIPLRGSNADN"
     gene            complement(21466..22047)
                     /locus_tag="ACHK1J_001427"
     CDS             complement(21466..22047)
                     /locus_tag="ACHK1J_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO7910725.1"
                     /translation="MNAEIMRLLENMVRTGVVSEVDLKNWRIRVTSGGLTTDWLRWNC
                     TRAGAFKIWLPPSPGEQVLLLCPGGSPEMAVVAGSLYSEEHPAPGTKQNEVILLAPDG
                     AEIRYDAEKGALTVKGIKTAEISAETRITLDTPAVECTKHLKVRTFELTDGGTLKGDI
                     THSNGNLRSNGVTVHTHVHNGVQSGGSMTGGPK"
     gene            complement(22044..22685)
                     /locus_tag="ACHK1J_001428"
     CDS             complement(22044..22685)
                     /locus_tag="ACHK1J_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO7910726.1"
                     /translation="MAPDDLHRLDGLFQEILSGLSAAGINKTARAVGQAVRAAQQKRI
                     RSQQSPDGNRWPARKKRVFRVQAGVVFIWNGTQVRRLKNWRTTTGRYGPMITGYDTER
                     RGIRSFHKADIEEYLEIDTRRRTQSAIKRPPMFEQLRTNRFLKVKTDPGGVSVGFEGR
                     AARIARVHQFGEVSLVSAGNAVRYPQRELLGFSEADRQKVTEIIINNLWRNTR"
     gene            complement(22672..23139)
                     /locus_tag="ACHK1J_001429"
     CDS             complement(22672..23139)
                     /locus_tag="ACHK1J_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7910727.1"
                     /translation="MLKINTLRDAMTDACPWCRANPEKFTVYVNNGGIETTGESPSYL
                     YRYTLGIFVMDFTQDLDELMVPLMRWLYRNQPDLLLNPEKNRDIKFSVAINDDDSTDI
                     LLEIPVWERVIIRRNDDGTVHAEHIGEPPVSRALEAWRVTLEDVTWGQNDGSG"
     gene            complement(23155..23328)
                     /locus_tag="ACHK1J_001430"
     CDS             complement(23155..23328)
                     /locus_tag="ACHK1J_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7910728.1"
                     /translation="MLFIHQIMLYFCTAVCALYLISGGYSVIRNYFQRKIDERAAEKI
                     SQKSAQQNNQNMP"
     gene            complement(23475..25520)
                     /locus_tag="ACHK1J_001431"
     CDS             complement(23475..25520)
                     /locus_tag="ACHK1J_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7910729.1"
                     /translation="MVPKHYQLVTAMTNKELAEKLAPMLREGWGLLGGPVSASEPGAP
                     FYLMQALVTDRDLPSAIPVPEGEEKPVPGTTDGYNTGHTTEPEFYYVVPLAGQSNGMA
                     YGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLNHP
                     NADLSKGQYGCVGQGLHIAKKLLAFIPKNAGILLVPCCRGGSAFTSGAEGAFSESSGA
                     TAESSLWGVGKPLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFL
                     NMVNQFRADLSAYAAQCTGGNASLVPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQK
                     IYFVPLMTDEKGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRG
                     IISDRLATAILMHAGKTVAFLKGAPAPFSVVAVPPATAGSDSTTTESPQPSTPAPQPG
                     AGTQSSPARTTAVAYRADAQEGKLKEQGWTGADGKASVVNDSAAESGKAMRVEKVQGQ
                     QTAWKIAHDVPASKAAELLQKGGEIAFRFKIPEGVQLAANRFAMALYWPVSQLPENVV
                     PEGSTGSNMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSIS
                     VTPVINGQTQAAFNLAKCPAAGLDNDKLLITDITSNQDTYPVLIEYVTVDINNSNAA"
     gene            complement(25531..25662)
                     /locus_tag="ACHK1J_001432"
     CDS             complement(25531..25662)
                     /locus_tag="ACHK1J_001432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910730.1"
                     /translation="MIWADVADWGDRLVDALDSCNADKAAIAGLERQRTARMNTHEK"
     gene            complement(25649..26074)
                     /locus_tag="ACHK1J_001433"
     CDS             complement(25649..26074)
                     /locus_tag="ACHK1J_001433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO7910731.1"
                     /translation="MKLNIILCALLLTISLQAMVVIRAYRSGQESTRYEALEKAVKES
                     NTTVRELLKATAETQSALAAIRREDQERAARGEERRETMRKNLQADECARTSPPADVA
                     DSLQQHASAAENRRHLRERAGKSDQTDAGTGAAATHDMG"
     gene            complement(26071..26613)
                     /locus_tag="ACHK1J_001434"
     CDS             complement(26071..26613)
                     /locus_tag="ACHK1J_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7910732.1"
                     /translation="MAGPKRYAVAAIVALGISLAPGELRTSREAQIKIATWEECRARP
                     YRDLVGVGTVGCGSTGNVENRLYTQEEVAGRWINDMRRAENCITQNFRGQQMPQSAFE
                     AMTDAAFNLGCRNLMWFKNKNGTPQRTTIWKHAQARQWRLMCYRLTDFVNSGGTRTQG
                     LVNRRNDFKNWCLKDAGAQP"
     gene            complement(26600..26884)
                     /locus_tag="ACHK1J_001435"
     CDS             complement(26600..26884)
                     /locus_tag="ACHK1J_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7910733.1"
                     /translation="MFQRLCDHITFWASVTTAGIGVMTLSEKIAVAGFVLGTLSLLRA
                     WLHRRRMEIAQARRNELIEKILMQSEGRELNDAERRAMMLLKQDEHDGRT"
     gene            complement(26887..27087)
                     /locus_tag="ACHK1J_001436"
     CDS             complement(26887..27087)
                     /locus_tag="ACHK1J_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO7910734.1"
                     /translation="MKVMALDGDTVDLLCQRHYGTTQGVTEAVLAANPGISAVMFLHA
                     GQEVELPELQGQTRREMIQLWG"
     gene            complement(27087..27581)
                     /locus_tag="ACHK1J_001437"
     CDS             complement(27087..27581)
                     /locus_tag="ACHK1J_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO7910735.1"
                     /translation="MSLIARPRVNSADTDTTDTDDGDAMVSAGDFWPAISLHEIRLAS
                     RIPGRTTTTRLLHAATEAVAHVIAELELWKRERMAEGAASLADVPATQVNGESVHVHR
                     YRRAVFALTRAFVLERTRDVDTTDKGDRRADALDMQVEDLWRDARWAISDIRGVTRIY
                     AELV"
     gene            complement(27686..28483)
                     /gene="gpM"
                     /locus_tag="ACHK1J_001438"
     CDS             complement(27686..28483)
                     /gene="gpM"
                     /locus_tag="ACHK1J_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO7910736.1"
                     /translation="MLTPAQRYVQQAVARNRVTMNARETQRERTAHEQVLHQLRLAQM
                     QLKGIQSNVARAELKKHLLPDFEGWIEGTLAGDSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLAEEITDAVLTQATTDSEADLSVYTAMLDELNHL
                     VSEQDMPDQVRSKLHKARAFSRRASSSPDDLRLSLSLLREAMRINPASGVKREITTLE
                     RAVRKLADGNQVVPASTTGRKTTTGGKKSSAKKKSAASQKKAARKTN"
     gene            complement(28524..29624)
                     /locus_tag="ACHK1J_001439"
     CDS             complement(28524..29624)
                     /locus_tag="ACHK1J_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO7910737.1"
                     /translation="MARLSLSREGRDSLERYMIQQASLAGITTERLGKTFSVDPSIQQ
                     KMENAIKESAGLLQEINFIGVDDQEGEKVLIDTSGPIASTNATSDGVKRRNPASVADL
                     AARRYRCEQVNYDTFISYAQIDAWSSQKNFQQLLSAQITRQIALDRIMIGFNGESHAL
                     ISDRATNPLLQDVNPGWLKHIRDKAATRVVKGMTLTRRDDENKLVAKGDYGNPDAMVH
                     DIRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGQLIVASRTV
                     GGLQTYLAPYFPKDAMLITSLKNLSIYFQKGSLRRFIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEGITFAGEDPDNNAESGQPQPEA"
     gene            complement(29642..30478)
                     /locus_tag="ACHK1J_001440"
     CDS             complement(29642..30478)
                     /locus_tag="ACHK1J_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO7910738.1"
                     /translation="MGSKNTPERKKFRVAVSGSTIDGREISGEMLRQAAENYDPSVYA
                     SRVNIEHVLSMWPSSELCAVGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMIAD
                     GKKVYSSIELLPELDAVKGPYIVGLAMTDTPASLGTERLEFAAQQRASIMQFSKCTTE
                     PPMFTVAMEAEMLTTTEQRTEESRQWFSRVMTLISKTRTADGEQFSQLRDVVENIASD
                     HADLIDRFSTLEQKHAIDKTALDELSHELAALKAQLRNQDADPQQRFTSAGGTDQTLA
                     EF"
     gene            30634..32367
                     /locus_tag="ACHK1J_001441"
     CDS             30634..32367
                     /locus_tag="ACHK1J_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO7910739.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLLTRQLKKLNEGQGEQAVSGKKP
                     RRRKLKNHFTEEQIIALRERTLRTLACHQREWYENRHHRNRMILKSRQIGATWYFARE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTGHLKFDEFFWVSNFVELRKVAGAMATLEGLTRTYFSTPSGET
                     HEAYQFWTGDRWNSEQPRSKRSEFDVSHAHLKGGVLFPDETWRQIVTIEDAVANGWRY
                     TNIGVIRGENTESEFRNLYMCEFVRDGEAAFSLSALTGCGVDGYDEWPDWKPFAARPM
                     GVREVWLGYDANGSSGKGDCGALSVCVPPLVAGGRFRTVETVRVQGMEFEQQAALIEE
                     YTQKYNVKHIGIDVTGGNGEGVYQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGR
                     WEYDRSERDLIQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAE
                     REDNRGFAMEF"
     gene            32373..33416
                     /locus_tag="ACHK1J_001442"
     CDS             32373..33416
                     /locus_tag="ACHK1J_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7910740.1"
                     /translation="MSKRKKYTGKRGQDSLHDALKSTPALSAFTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGTEFSFRKG
                     TVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTPQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGVEVM
                     RFNPYALLLDNAS"
     gene            complement(33482..33985)
                     /locus_tag="ACHK1J_001443"
     CDS             complement(33482..33985)
                     /locus_tag="ACHK1J_001443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910741.1"
                     /translation="MSHFRDLYLANKKSRDARREVTGILRNQAEKLVAFYEQWLELPS
                     ETWEDKDGEHHRYVDTGFPCRNDRDFRPFPVNMIATDTDNIFRMAVRTWVDSHDEGES
                     VNIVLAMELHSVGKDGVSLRVRVEDDTSIRVVINGDGDREWLPIVNSMKMHIISKLQR
                     RTPSAFR"
     gene            complement(34521..34898)
                     /locus_tag="ACHK1J_001444"
     CDS             complement(34521..34898)
                     /locus_tag="ACHK1J_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7910742.1"
                     /translation="MKARIGIIPEKVLRQRMLDIAKGKLKPEPDEPRVWFSSLNALGQ
                     ALSNENIALLRIMDAEKPQTMTELASLSGRQLSNLSATLKMLSRYGFVSLEKQGNCVQ
                     PKALFTDFEIIIDPSVGEAHRAA"
     gene            complement(34895..35269)
                     /locus_tag="ACHK1J_001445"
     CDS             complement(34895..35269)
                     /locus_tag="ACHK1J_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015834182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HFO7910743.1"
                     /translation="MKNNDHGLNTLLDMHGYQHHMDNGYWWKIEAYRVSPSQFRPHGI
                     RYNLTLHDPYNSRVFGMDNAHGIKPPKKGKFTGRITVYDHIHRTSSDKGYPYEFTSAE
                     QLLLDFFNHVDRVIAEQQRGKK"
     gene            complement(35524..35841)
                     /locus_tag="ACHK1J_001446"
     CDS             complement(35524..35841)
                     /locus_tag="ACHK1J_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7910744.1"
                     /translation="MTTQIATRGRLAEDPTTRKSSRGTDVTAARLAISLPGHGAEQST
                     TMWLYLIAFGKQHEELAKHKKGDLVQVHGNLQVNRYQDKYGQMKEGWQLLAQSIISSR
                     TAI"
     gene            complement(35865..36212)
                     /locus_tag="ACHK1J_001447"
     CDS             complement(35865..36212)
                     /locus_tag="ACHK1J_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO7910745.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEEERLELAQRAEWQQEKSVIEVCKRSEVMKRILFI
                     YGAGGDLPVISVQ"
     gene            complement(36209..36403)
                     /locus_tag="ACHK1J_001448"
     CDS             complement(36209..36403)
                     /locus_tag="ACHK1J_001448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910746.1"
                     /translation="MSTRQQHTINSTRAISDRDLNSMIESAQQLLSRQNPVVEETWWE
                     NLLAALTELRERRNNEGATK"
     gene            complement(36423..36824)
                     /locus_tag="ACHK1J_001449"
     CDS             complement(36423..36824)
                     /locus_tag="ACHK1J_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910747.1"
                     /translation="MTRFNCTLQQREFSVVMTKRLPALTLRGLIRRPQPGDQLEITIA
                     RPDRSTESMTADILNARPWGDSGDFIAALRLRNTGAPEPLTPAGRIMKAYHEKDIRKH
                     NEDLARAMKRINRKAAIASYVHSAARKQHRK"
     gene            complement(36821..>37429)
                     /locus_tag="ACHK1J_001450"
     CDS             complement(36821..>37429)
                     /locus_tag="ACHK1J_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7910748.1"
                     /translation="YGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTDE
                     GFDVECGFAAPWTRGNNCRHDEKTNNETDKTPPPEGRKEQKLIVPEGDPGEWLRSLTP
                     VQRKQLTQQLKNDQKSTKTANSGGFPAPEGVVYAGAALAKPDMTTRLIASTLSTTGEE
                     LHSSAALSVYRGARVRLPDGRIVHWDESTGSLKEVAQLKGDE"
BASE COUNT         7919 a         9750 c        10383 g         9377 t
ORIGIN      
        1 taattgatga tgattttgcc atagtcatag aatctttggg ctttgcgctt aaaatctatg
       61 gaggggatga acgttttcgt aatggttcct ctgttgtttt ggaggatgaa gattactctc
      121 ttatttataa ttttctggtt aatgcaggtt gccaacaagt cgaattagcc gaacaagtgg
      181 atgcgatagt aagtgcgaat ctggccgcag atagtgatat taccaaagaa aagatttgcg
      241 agaaatataa atcgaccatt gaggcgttta aaaaagagca actagcgtta ccagtattag
      301 ttcggcgtaa gaactcagag acttaattcc cctatcccat agataacgat aggggaaaat
      361 tatttttgca gaatttatgt tattgccatt atttgagcaa cttttgcagc aggtccatca
      421 cttgctgcac ctcttcctga gtgagcgccc cgtctttggc ccattgcacg cgaccatcct
      481 tatccagcac cactacagcg gaactttctt catccagctg ccaggcaccg cgcgcgacgc
      541 cattgctatc gacaataaac tgcgaccagg gataaagctt tttattactc tccagactgc
      601 tgcgcacaaa catgccggaa cccggaatcg cgtcgtcggt gttaacaatg gtggtggtct
      661 ggtaacgatc gtgcggtaac ttcgctgatt taatcgcttc aatcagcgtc gcgtttttct
      721 cttttgcaga ggtgcgacca gcaatatgtt gcagtactcg cacttttccc actaactgcg
      781 cgctgttcca ggttttgtag ctaaactgat ctttatcaag caccaactcg ccccgatcgg
      841 taatgccaat cggcggcact cgctgaccgg tttcgaactg atgtgcggaa gccatcatcg
      901 gcaacagcag gcaggtgagt gccagaatct tgcgtagggt catggtgttt ccttcttatg
      961 atatgcaggt gatccgacca cttgggtctg agttttaatc atatgtgcta tttatcgaat
     1021 ttcccgcaag tgtgatgcca gtttgcggtc aagcgcacaa atcatatgaa aaatgaatgc
     1081 ttatactgaa gaccgcgctt cggtaaaaag ataattctga ataattgtaa cctttaggta
     1141 aaaaaagtta tacgcggtgg aaacattgcc cggatagtct atagtcacta agcattaaaa
     1201 tttgcgcctc ataatagttg ggccgattgt ggcaccgcac aggcgtaata ctcagcagga
     1261 gataacaatg aaaattttcc aacggtacaa cccacttcag gtggcgaagt acgtaaagat
     1321 cctgttccgt ggacggttat acatcaagga cgttggcgct tttgaatttg ataagggtaa
     1381 gattcttatc ccaaaagtga aggataagct gcatttgtca gtgatgtccg aagttaaccg
     1441 tcaggttatg cgtctgcaaa cagagatggc ttaaccaaag tgctatgcag taataagacg
     1501 gctcctgatt caggagccgt tgatgtttat gggggttacg cgacgctttc ttctttatct
     1561 ttagcgtcag gcagttttgg cgaaagggct gtggccttgc tgtcgatccg ggtcaccagc
     1621 agctggtcga tgcggtagtt atcgatatcc accacttcaa atttgtagcc ggcgaatttc
     1681 accgaatcgg tgcgtttcgg gatcttacgc agcataaaca tcataaagcc gccgatggtt
     1741 tcgtagttgc ccgactgcgg gaactcgtca atatccagca cgcgcatgac gtcgtcaatc
     1801 ggagtgccgc cgtcaatcag ccatgagttc tcatcacggg cgacaatctg ctcttccagt
     1861 ccctgaccga ccagatcgcc catcagcgtg gtcatcacgt cgttgagggt gatgatcccc
     1921 actaccagcg cgtactcgtt catgatcacc gcgaagtctt ctcctgcggt tttaaaactt
     1981 tccaacgcct ctgaaagggt taacgtatcc ggcacaatca gcgtgttgcg aatttgtacg
     2041 ccgctgttca gtgccaggct ttggttagcc agcacgcggt tcagcaggtc tttagaatcg
     2101 acatagccga tgatgtggtc aatatcttca ttacagacga ggaacttaga gtgcggatgt
     2161 tccgccacct tatttttcag actttgctca tcttcgtgga gatcaaacca aatcacgttt
     2221 tcacgcggtg tcattgaaga cggaacggta cgggattcca gctcaaagac gttttcaatc
     2281 agctcgtgtt cctgtttacg taacacgccc gccagcgcac cggcttccac taccgcgtag
     2341 atgtcatcag aagtgatgtc atctttacgt accattggca gtttgaaaat acggaagatt
     2401 atgttcgcca ggccgttgaa gaaccacacc agcggggtgc aaacgtacag gcagaagcgc
     2461 atcgggttga tgatacgcaa agccacagct tctggcgcaa tcataccgat gcgtttcggg
     2521 gttaaatccg caaacaggat aaacatgcca gtcactaacg agaaagagag aataaagctc
     2581 agttgctcag agagctctgc cgacatatag cgggagaaca ggctgtgaaa agctggagaa
     2641 aatgccgcat caccgacgat accgccgaga atcgccactg cgttcagacc gatttggacc
     2701 acggtaaaga acatgccggg attttcctgc atattcagaa cgcgttgggc atttatattg
     2761 ccttcatcag ccagcagttt aagtttgatt ttgcgtgagg cggcaagcga gatctcggac
     2821 atcgagaaga acgcacttac agcgatcaag cagagtatga ctaaaatact gtttaacata
     2881 tcttatccga cctttcaggc cagatcctcg gaaggggaag ttgattaatt tgtgtgaaat
     2941 acacattgaa agccgattcg aagaaagtga accggcattt tcagtggcta gtatagcgta
     3001 aggtactgta aagtcgccag agggttaaaa ttcagactgc tgataaatgc aatactgcct
     3061 gatgcgctat gccgatcagg cctacgcagt ccttgcaata tattgaattt ggacgatttt
     3121 gtaggcctgg taaggcgttt acgccgcatc cggcataaat aaagcgcact ttatcaacaa
     3181 tctacatttg tacccgtaac gctatgcttc cggtggcaga cagacgccaa ttccgccaat
     3241 tccacagtaa ccatacgggt ttttatgcag atattgctgg tggtcatctt cggcataata
     3301 aaacggtgtg gcgttagcga tttccgtggt gatgtgacga tcgtcatcgg cggcaagcat
     3361 cgccgcctga aaacgttcca gactggcgcg agctgcggca tcctgttctg gggtcagcgg
     3421 ataaatcgct gaacgatact gcgtgccgtg gtcattgccc tgacgcattc cctgggcggg
     3481 atcgtgattc tcccaaaata cctgtagcaa ctgctcatag ctgatgacgg aaggatcgta
     3541 aacaatgcgt accgcttcgg catgacccgt atcaccggag cacacttccc gataagtcgg
     3601 atttggcgtg tagccgccgg tatagcctgc ggcggtgctg taaacgccgg gtaactgcca
     3661 gaacagacgc tccacacccc agaaacaacc catcgcaaaa atggcaatct ccattccgtc
     3721 aggtacattg gtcattgagt gaccgttgac cgcatgcagc gtggctacgg gcatcggggt
     3781 gttacgtcca ggcagggcat cggcggggga aaccagatgc tttttatcaa ataaactcat
     3841 ggtgtcgctc tcccgaaaat cggtcattgg ggttaaggtt gtaacaagag gcgtatttgc
     3901 acacaataac caccgtgaat aggtctaaag taaaacataa gaaatatttg gggtttagtc
     3961 tgctttttaa tccatattac tggatttttg ttaagccgtt taacggcgtt ccaggggcag
     4021 gaaaaaagga tattcaggag aaaatgtgcg ctatatccga cagttatgct gtgtaagctt
     4081 actctgctta agcggatctg ccgtcgccgc gaacgtccgt ctacaggtcg aggggttatc
     4141 gggacagctg gaaaagaacg ttcgcgcgca gctttctacg attgaaagtg atgaagtgac
     4201 gccagaccgt cgctttcgcg cacgcgtcga tgatgctatc cgcgaaggtc tgaaagcgct
     4261 gggttattac cagccgacca ttgaatttga tctccgtcca ccgccaaaga aagggcggca
     4321 ggtattgatc gccaaagtca cgccaggcgt gccggtgtta attggcggca ccgatgtggt
     4381 attgcgcggc ggcgcgcgga ccgataaaga ctatttgaaa ttgctcgata ctcgcccggc
     4441 tattggtacg gtactgaacc agggcgatta tgaaaatttc aaaaagtcct taaccagcat
     4501 tgcgttgcgt aaaggttatt tcgatagcga atttaccaaa gcgcagctgg gcattgcgct
     4561 cggcctgcat aaagccttct gggatattga ttataacagt ggcgaacgtt accgctttgg
     4621 gcatgtgacc tttgaaggat cacaaatccg cgatgaatac ctgcaaaatc tggtgccgtt
     4681 taaagagggc gatgagtacg aatcgaaaga tctggcagaa ctgaaccgcc gactttctgc
     4741 taccggctgg tttaactcgg tggtggtggc tccacaattt gataaagcgc gcgaaacgaa
     4801 agtattacca ttgacgggcg tggtttcgcc gcgaacagaa aacaccatcg aaaccggggt
     4861 cggttactct acggacgtgg gaccgcgcgt gaaagcgacg tggaaaaagc cgtggatgaa
     4921 ctcttatggt cacagtctga ccaccagtac cagtatttcc gcgccggaac agaccctcga
     4981 cttcagctat aaaatgccgc tgctgaagaa tccactggaa caatattatt tggtgcaggg
     5041 cggttttaag cgcactgacc tgaacgatac cgagtctgac tccactacgc tggtggcttc
     5101 tcgctactgg gatctctcca gcggctggca gcgtgccatt aacctgcgct ggagtctcga
     5161 ccactttact cagggtgaaa ttaccaacac cacgatgctg ttttatcctg gggtgatgat
     5221 tagccgcacg cgttctcgtg gtggcctgat gccaacctgg ggcgactcgc aacgctactc
     5281 tatcgactac tccaacactg cctggggctc agatgtcgat ttctccgttt tccaggcaca
     5341 aaacgtctgg atccgcacac tgtacgatcg ccatcgtttt gtgacacgcg gcacgctggg
     5401 ctggattgaa accggtgatt tcgacaaagt accgccggat ctgcgttttt tcgccggggg
     5461 cgaccgcagt attcgtggct acaaatacaa atctatcgct ccgaaatacg ccaacggtga
     5521 cctgaaaggg gcctcgaagt tgataaccgg atcgctggag taccagtaca acgtgaccgg
     5581 aaaatggtgg ggcgcggtgt ttgtcgatag tggcgaagcg gtaagcgata ttcgccgcag
     5641 cgactttaaa accggtaccg gggtcggcgt acgctgggaa tcgccggtcg ggccaatcaa
     5701 actcgatttt gccgtaccgg tcgcggataa agacgaacac gggttacagt tttacatcgg
     5761 tctggggcca gaattatgag tttatggaaa aaaatcagcc tcggcgtggt tatcgttatc
     5821 ttactgttgc tgggatcggt ggcgtttctg gtgggcacca ccagcggcct gcatctggta
     5881 tttaaagcgg cggatcgctg ggtgccagga ctggatattg gcaaggtcac cggcggctgg
     5941 cgcgatctca ccttgtctga cgttcgttat gagcagccag gcgtggcggt aaaagcaggc
     6001 aatctgcatc tggcggtcgg gcttgagtgc ctgtggaaca gcagtgtttg tattaatgat
     6061 ctggcgctga aagacatcca ggtcaatatc gacagcaaaa aaatgccccc ttctgaacag
     6121 gttgaagaag aggaagatag cggtccgctg gatctctcca cgccgtatcc catcaccctg
     6181 acacgggcgg cactggacaa cgtcaacatc aagattgatg acaccacggt atcggtgatg
     6241 gacttcacct ccggcctgaa ctggcaggag aaaaccctga ccctgaaacc gacgtcgctg
     6301 aaaggcctgc tgattgctct gccgaaagtg gcggaagtgg cgcaggaaga agtggtcgaa
     6361 ccgaaaattg aaaatccgca gccggaggaa aagccgctcg gcgaaacgct gaaagatctc
     6421 ttttctcgcc cggtattgcc ggaaatgacc gacgtgcatt tgccgcttaa cctgaacatt
     6481 gaagagttta agggcgagca gttgcgcgtg acgggcgaca cggacatcac cgtgcgcacc
     6541 atgctgctga aagtgagcag cattgacggc aatactaaac tggacgccct ggatatcgat
     6601 tccaaccagg ggatcgtcaa cgccagcggc acggcgcagc tgtcagacaa ctggccggtg
     6661 gacatcactc tcaacagtac actgaacgtg gagccgttga aaggtgaaaa agtgaagctg
     6721 aaagtgggcg gcgcgctgcg cgaacagctg gagattggcg ttaatctttc cggtccggtg
     6781 gatatggatt tacgcgccca gacgcgactg gcggaagccg gattgccgct caacgtggaa
     6841 gtgaacagca aacagattta ctggccgttc actggtgaga agcagtatca ggcggatgat
     6901 ctgaaactga aacttaccgg caaaatgacc gattacacgc tctctatgcg tacggcagtg
     6961 aagggacagg agatcccgcc cgcgactatt accctcgacg ccaaaggtaa tgaacagcag
     7021 gtcaatctcg acaaactcac cgtcgcggcg ctggaaggga aaactgaact caaggcgttg
     7081 ctcgactggc agcaggccat tagttggcgc ggtgagctaa cgcttaatgg cattaacacc
     7141 gccaaagagt tcccagagtg gccgtcgaaa ctcaatgggt tgattaaaac ccgcggtagc
     7201 ctgtacggcg gcacctggca gatggacgtg ccggagttaa agctgaccgg taacgttaaa
     7261 cagaacaaag tgaacgttga cggcacgctg aaaggcaaca gttatatgca gtggatgatc
     7321 ccagggcttc atctggaact cgggccaaac agtgccgaag tgaaaggcga gctgggggta
     7381 aaagatctca atcttgatgc caccatcaac gcgccggggc tggataacgc gctgccgggg
     7441 cttggcggta cagcgaaagg gctggtgaaa gtacgcggca cggtggaagc gccacaacta
     7501 ctggcagata tcaccgcgcg cggcctgcgc tggcaggaac tttccgtggc gcaggttcgc
     7561 gtggaaggcg acattaaatc caccgatcag atcgccggga aactcgacgt acgcgttgag
     7621 caaatttcgc agccggatgt aaatatcaac ctcgtcaccc tgaatgccaa aggcagcgaa
     7681 aagcagcacg agctacagtt gcggattcag ggcgagcctg tctccgggca gcttaatctg
     7741 gcaggaagtt ttgatcgcaa agaagaacgc tggaagggaa cacttagcaa tacccgcttc
     7801 cagacgccgg tcggcccgtg gtcgctgacc cgcgatattg cgctggatta ccgcaataag
     7861 gagcaaaaaa tcagcatcgg gccacactgc tggcttaacc cgaatgcgga actgtgcgtg
     7921 ccgcaaacta tcgatgcggg ggccgaaggg cgtgcggtgg tgaatctcaa ccgcttcgac
     7981 ctcgccatgc tgaaaccgtt tatgccagaa accactcagg ccagcggtat cttcacgggt
     8041 aaagcggacg ttgcctggga catcacgaaa gaggggctgc cgcagggcag tatcactctt
     8101 tcggggcgta acgttcaggt aacgcaaacc gtcaacgatg cggcgctgcc cgtggcgttt
     8161 cagacgttga atctgtcggc ggagttgcgt aacaaccgtg ccgaattggg ctggaccatc
     8221 cgcctgacca ataacggcca gtttgatgga caggtgcagg tgaccgatcc gcaaggccgc
     8281 cgtaatcttg gtggcaacgt caatatccgt aacttcaacc ttgcgatgat aaaccccatc
     8341 tttacccgtg gggaaaaagc agcggggatg gtgagtgcca acttgcgtct gggtggtgat
     8401 gtgcaaagcc cgcagttgtt tggtcagctt caggttacgg gtgtggatat cgatggcaac
     8461 tttatgccgt ttgatatgca accgagccag cttgcggtca actttaacgg tatgcgctcg
     8521 acgcttgccg gtacagtacg gacccagcag ggtgaaatct acctgaacgg tgatgccgac
     8581 tggagccaaa ttgaaaactg gcgggcgcgg gtaacggcga aaggcagtaa agtgcggatc
     8641 accgtgccgc cgatggtacg aatggatgta tcgccagatg ttgtattcga ggctacacca
     8701 aacctgttta ccctcgatgg tcgcgtggat gtcccgtggg cgcgcatcgt ggtgcacgat
     8761 ctgccggaaa gcgcagtagg cgtctccagc gatgtggtga tgcttaacga taacctgcaa
     8821 ccggaagagc cgaaaacggc gtcgattccg attaacagta acctgattgt ccacgttggc
     8881 aacaatgtgc gcattgacgc ctttggcctg aaagcgcggc tgacgggcga tcttaacgtc
     8941 gttcaggaca aacaagggct gggcctgaac gggcagatca acatccctga agggcgcttc
     9001 catgcctatg gtcaggatct gattgtgcgt aaaggtgagt tactgttctc tggcccgccg
     9061 gatcaaccgt atcttaatat tgaagctatt cgtaacccgg atgctacaga agacgacgta
     9121 atcgccggag ttcgcgtcac tggtctggcg gacgaaccga aagcggagat cttctctgac
     9181 ccggcgatgt cgcaacaagc tgccttgtct tatttgctac gtggacaagg gctggagagc
     9241 gatcagagcg acagtgcggc aatgacctcg atgctgattg gtctgggggt tgcgcaaagt
     9301 ggccagattg tgggtaaaat cggcgagacg tttggcgtaa gcaatttagc gctcgacacc
     9361 cagggagtag gcgactcctc ccaggtagtg gtcagcggct atgtattgcc aggtctgcaa
     9421 gtgaaatacg gcgtgggtat atttgactct atagcaacac tcacgttacg ttatcgcctg
     9481 atgcctaagc tatatctgga agccgtgtct ggtgtagacc aggcactgga tttgctctat
     9541 cagttcgagt tttagcaatg cgaatatttg tctacggcag tttacgccac aaacaaggca
     9601 acagtcactg gatgaccaat gcccagttac tgggcgattt cagtatcgat aactaccagt
     9661 tgtatagcct gggccactat ccaggcgcag ttccggggaa cggaacggta cacggtgaag
     9721 tttatcgtat tgacaacgcc acgctggccg aacttgatgc cttgcgcacc aggggcggtg
     9781 aatacgcgcg ccagttgatt cagacgccgt acgggagtgc atggatgtac gtttatcaac
     9841 gacccgtcga tggattaaag ctaattgaaa gcggcgactg gttagacagg gataagtaac
     9901 cctatgcata cgccaccttc gggtggcgcg aaaattgatg taatacaatg aaaaataaag
     9961 gattattttt cgagttgtcc acatgttgcc atggcgttct aaattttttt ggtgagacta
    10021 tctctgcttg ttgttttcac cgccagcttg ttaaacaaaa aaagcccaca gtttttacgc
    10081 tgcgggcttt gtttttcttt gtgacttgcg tgtgagggtg cgactttcga ccgaaccttc
    10141 ggcaaccgga tagcgggggc gttcaccccc gctgcggttt cttacggttt acactgcaag
    10201 aacgccgcaa attctgacca catgatgtta agcctgaact cacacggcga actgtgtatc
    10261 atccagacag tcaacaacat ggttaagcat aaaatgctca cgatgatcgt tttttgcggc
    10321 atagcgcttg ctcctttttc aaagaggcgc taaccttcca cttgtctaag gtaggatggt
    10381 tagggcctcg ggttaaacaa tatgtttgac tcggggcctt tccacatccg gccttcaggt
    10441 tttccttcca gccgtcagcc gaaaggcacc cgcgcgtaat gtactggttt tgaggatatg
    10501 ccgcaatcct ccagttgtag cgttaactga ctgcgtccct gatgggattt gcgcattgtc
    10561 tcccgtggaa tatctttatc gtggaagatc aaatgaggct ggccaataat tctgtgcaca
    10621 ctttccattg ttgaaaatgt ggcgctgcac tcaaggttat tgcactgata gtatttgtag
    10681 cggatggtgc cggtatcatt ggcggcttta ctggtacgga tccgggcaga tgcgccgcag
    10741 aatggacaat gaaacattga tataccccct ggctgaactg gggttgaact cgtcgccatt
    10801 ctaaccattg ctttcctttt cggctatcca ctcgggtatt ctggcctcca gttcaagcct
    10861 tgtggtgaag ccgcttccgt cgatattgtg ttccactttt gcaatgagcc agtcctgatt
    10921 gtctatctcg gttttaaagc cggttaccgt gccatgcatt tcagggtaaa gctccgctct
    10981 gccccgggcc agcgtgatgg agaattcagc cgcaccgcgc tgtaattgct gccacctggc
    11041 ggcagctgcg cgctgtgcag caatttcatt tgggtaggtt tttctcagaa cgaacacgtt
    11101 accttctgcc ccctccatgt agctgccttc ccggctgctg ctcttttcag cgtgagtttt
    11161 tcgttttttt atgttgacgg ctttcttttt gccatgattc aaatccagcc agtacgcctt
    11221 cacccctgtg tatgcctggc gatcggctat tctgaactgg tgactgtctc cgtcactgcg
    11281 tgccagtgca aatgagggga gtgggcgccc gtcagctgtc acgccgccac ccggcagaat
    11341 gaacagcaga cagccatttt tgacggtagt gatggctccc agcatatcag ccatgcgggt
    11401 aaggaatgat atgtcgcttt cttccgtctg gtcggcatga tcgatttcaa tatccataag
    11461 catatcgctt atctgggctt tcagtccgta acggtgggca atagcggaaa ccacgcgttc
    11521 aacagtcacg tcgtgccagg atacttcccg tttgacgtta aattcctccc tgaagtctgc
    11581 gctgtttgca ctcagtgtca gccgatccgg aggtccttca tgagcgacct catcaacaat
    11641 gtaggtgcct ttatcaacca gaggctctcc cttccatccc agcatgactc tgatgtgtgt
    11701 tccccgcccg gggagagcca gctgcccgtc ggcatcatcc agcacaagag tgaccctgtc
    11761 tgcttcaaac ccccggttgt ctgtcatgga aagcgatatc acccggttat tcagttcctg
    11821 aagtgtgttt ttatcctgct ccctgtccag tgaaacgctg aaatcaggca ttttcaccag
    11881 ttctttcagg ggggaataaa ccgtgttacc gttcagcaat accataattc ctccgttttt
    11941 cccgccacag ttgcatacca gaaacaccac cggaatgagt ttgggttgta tgtgtgttgc
    12001 gagaaaccgc agttcgtgaa aaagctcccc gtgttcgtaa agatctctgc tgaactcaca
    12061 cgaatgcagg gtgaataata tggctgaaac ccgtttccat ggcgtgcggg tccgggagaa
    12121 tgcggatctg gtcacggcca ttaatgatat tgactccagt gtgattggta tcgtggctgt
    12181 tgctgatgat gctgatgcaa aaacatttcc gctggataag ccggtgctcc tgacgcgcgt
    12241 tatggatgtg ctgggtaagg ccgggaaaac cggcacgctg tacaaaagtc tgaaagctat
    12301 tgccgatcag gtcagcacca gagttgttgt ggtgcgggtt gctgctgcga aggaggggga
    12361 tggtcagccg acacaaacgc agcttgttat tggtgaggtc aaaaatgacg gaacctatac
    12421 aggcatgcag gcgctcctgg tggctgaaca gaatgaggct gttggtgttc gtcccaggat
    12481 cctggctgtg ccatcgcttg acaccaaaga ggtagtggcg gcactggtaa ccgtcgcgaa
    12541 aaagctgcgt gcctttgtgt atgcgggctg tcccggctgt aataccgtgc aggaggccgt
    12601 tacctggcgc gccgatcttg ctcaccgtga agttatgctg atctggcctg attttatcgc
    12661 gtacaaccct aacagtggcc agaatgaggc ttttcctgca tctgcgtatg cgtgcgggtt
    12721 gcgtgcgaag attgaccacg agcagggctg gcataaatcc ctgtccaatg tggcagtgaa
    12781 taatgtcctg ggtatttctg tgccggtgtt ctggtcgctc caggcagagg acagtgacgc
    12841 ggaaaccctc aatgacaagg agatcaccac actcattaaa cgtaacggct tccgtttctg
    12901 gggtaaccgg actctggaca ccaccaccta cacctttgag acctacacgc gtacggcgca
    12961 gtcgctggcg gataccatcg ccgaatcgca gtttgaaacc gtggacaagc cgctgacgcc
    13021 gtcgaacgtg aaggatgtgt taagcgatat ccgggcaaaa ctgagtgcct ttgtgacagc
    13081 gggtaaactt atcggggcag agtgctggta tgacgtggtg gataacggca caacggatct
    13141 gcgtcagggg gttgtgcgca ttcgctacaa gtatacgccg gtgccaccgc ttgaagatct
    13201 tgagcttcac cagacgttta ccgatgaatt ctttgcgggt gcatttgcat cactgggagg
    13261 tgagtaatgg cggtaccaaa caaacttcgc gttttcacgg tgtacatcga tggcgaaaac
    13321 aagctggggc gggtgacgtc cttcacgccg cctaaactga cccgaaaaac ggagtccttc
    13381 cgtggtgccg ggatgcccgg cagtgtttcc gtcgactttg gtcttgatga tggtgcactg
    13441 gatctctctt ttgcggtagg cggcgcagat gcccaccttt tctcccgtta tgccgggtca
    13501 cttgatgaag tccgtcttcg cttttccggt gagtactatt cggatgcgga tatcagatat
    13561 gtggatattg aggtccgcgg gcgcatcact gaaatcgata tgggggaggc gaagcagggt
    13621 gaagacactg agcacagtta ttcgatgaaa aacacctggt acagactgag tgtggacgat
    13681 ctggaaaaaa tcgaatttga tgtgctgaat tttatctggc gcattgacgg taaggatgtg
    13741 ctgccggatc gcacccgctc gatgctgggc ttctgattat tgtttaacag agaacggcgg
    13801 ggatcccgcc gtggagactg aaaatggata aagaaacgga aatgaccact gacactgccg
    13861 tcagtaacgg ggaggcttcc gcggtggcgg ttgccgctac ggctgcacct aagaacgtgg
    13921 tgaagctgac ggtgcctgtt gtgcgcggag atgtgtcgta cagtgaaatt aaaatcggtc
    13981 cgctggcgaa tcaggccgga tcgctgcgtg ggctgtcttt gcttgcgctt tataatatga
    14041 actgtgatgc catggtcacc tggcttgcgc gtgtgacaga gccaaaactc aaggaggccg
    14101 aaatgtatgc catgaatgtg gcggatttta cggcgctgag ctcggcggcg gttaattttt
    14161 tcgcaccctc ggggtcgaat gcaccggcac cggagaacac ggcagagccg taacctgtct
    14221 ttgctttgac tgcattgaag atcccgttgc ggatattgct gttattttta actggccgcc
    14281 cgcagagata tttgcaatga cgcctgcaga ggtggtcgcc tggcgtgagc gggcggctgc
    14341 cagaagcgga agtcacaatc atgatgaata atctgcatat cagggttgct ctggcggcgg
    14401 cagacaccct gacacaacct ctcaggcgcg cccgcgaaac cgtgggcgcg ctttctgatt
    14461 ccctccgtga aactcaggac tccatccgct cgcttgaacg gcagagtgaa gcctttgctg
    14521 cggcacagac taaatttgac gaagccaccc ggaaaattgc cgctgttcag cagcaactga
    14581 accgtctgca gcaggcggag cgtgagggta ccgtgctcag tgaacggcag cgtgaaatga
    14641 tggcttcgct gaccacccgg cttgaacgtc tgaaccgggt gagggatgta catcagcgac
    14701 gcctggagga ttcacgccgg gagctggaga gacacggtat tgccgcagag ggcagcagtc
    14761 agaccataca ggcagccatt cgccggacgg cagagtataa cgaagagctg gcgcgacagc
    14821 ggcaggcgct cgcacaggtg acccgtgcac agcaacgcta tgaacaggcg caggagcgac
    14881 tcgggcggat acgtaatgcc ggtgcggcgg cactggctgc cggtgcagca ggactttacg
    14941 ctggcgggcg tgctgttgcg ccggtgatgg atctccagcg tcatggtgcc gtcattgcgg
    15001 ctcaggcagg agagggcgcg tcagatgccg tggggtatac cggggttata aaccacatgg
    15061 ttcataccgg tatcacaccg gatgcacaac tggcggctga agctgtgggt gctgtccgct
    15121 ccactctggg tgggctgggg gcggttagtg agaatgaact gacccgaatc acccgaaaaa
    15181 tgctggatat gcaggccgtg atggggggcg atatcccgca gcacattcag gcggctgcca
    15241 tcatgatcaa aaacggtctt gcacgcagca gtgacgaggc ggttgatctg atggtggcgg
    15301 gcatgcagcg catggggccg gttatgcgcg atgagctgcc tgagatcctt cacgagtatt
    15361 cgacccattt tcgtaacatg gggctgacag ggtcggaaac catgacgctc cttgcggata
    15421 tggcgcagca ggggaagttt gcgctggata aaaccggcga tgccatcaag gagttcagta
    15481 ttcgcggctc cgatatgtca aaggcgagtg ctgaagcgta tgcgcttatc gggctggatg
    15541 cagccaggat gtcatcggct gtagccagtg gtggaacggc tgcacgggct gcgctgcaga
    15601 aaacagcctc gggtctgttg cagatcgtcg atccggctga gcgggccaat gcggccatcg
    15661 cactttttgg tacaccggtt gaagatctgt ctgtcgatca aatccctgcc tttcttggcg
    15721 cgctggcgcg ggtgtctgac cgtatggctg acgtgtctgg tgtggcggac cggatggggc
    15781 aaaccctgcg tgacaatctg agtggtgatg tggcgcggct taccgggacg ttcagcgcgc
    15841 tgagaagtgg gattatcggg gagattacgg acgatctgcg tgggcttgtc cggatggcca
    15901 cagcgtgggg tgagcgtctg caggcgtggg tgaatgcgca tccggaactg gtgcgaatga
    15961 ttgcgctgac gacgggagcg gtgctggcgt taaccacagc gatgggggcg ttgtccgttg
    16021 cggcctggat ggttgtcgca ccgttactga aactgcgtct ggggcttgcc ctgcttcgcg
    16081 cgatcccggc cggactctct gttgccggta cgctgtttgg cggactgacg ggtgtgattt
    16141 cagccgtggg ggctgcgctg ggggcgctga cctggccggt tgtggtggcg gtgggagccc
    16201 tggtcggtgg tgctctgctg atctggcgct actgggatcg ggtcagggcg tttgtgagcg
    16261 gatttattga aggggtacag tcagcactgg cgcccttcat ggcgacagcc tcccgttttg
    16321 cgccgctgct ggatctggtt tcaggggcac tgtcccgtat tctgggatgg tttaaatccc
    16381 tgttaacgcc agtctcctcc acgcgtgatt tgcttgagca gtgcacctct gccgggcaga
    16441 cgttcgggag cactctggcc ggtgcgttag ccacggtttt ttcgcccctg aaagctctga
    16501 ttgacggtct ggcgtgggtg ctggaaaaac tcggtgttct gcccggtgaa gctgagcggg
    16561 ttaagcgaaa actggatgag gcccgtaaaa cgccggtggc atgggagtgg gatccggaac
    16621 agaaaaaaat ggttcagaag gcgtggcact ggtcgcctcc tggtggggcc agtgtgcctt
    16681 ccggcgcctc cggtacacag cctccgccgg tggcgctccc tgcggggata gcgccggacg
    16741 gtaaccgtaa cgttctccgt cacctgaaag gcattgaggg gaataccggt ggtctgctgg
    16801 ctgaaaaccg taaacgtatc ggacccggag atattgtgtt taaacatctt ccacgggcgc
    16861 tggcggtccg ggggcagtgg catgaacctg tcgttgcgcc gtcttccggc ggaatgcgcg
    16921 cgctgccaca ggaggagatt caggtgcagc aatggccgac ggcacgcccg gcgccacgaa
    16981 tgcaccagca ggaaaccttc gccagcgcct ttcaggggga gatacatgtg catcttcacg
    17041 gggtgagttc gcataatccc cgggaactgg cgcgcatggt tggtgacgct gtcagggcgg
    17101 aaatgaacag aatggcacgt gccggaaccg gcagcttccg cgatcgcgac tgaggtgaaa
    17161 aagatgatga tgatttatgg gatgtttgtg tttcagttaa gtaccctgcc gcatcagcag
    17221 gtggaacaga gtaaacgctg gcgacacgtc agtaatgaac gggttaaccg ttcatcctcc
    17281 tggcagtata ttggctctgg tgaagatcac atcacgatgt ccggtgtgct ctgtccggaa
    17341 atcaccggcg gggaggtgtc actgactgcc ctgaacaccc tggcctacac aggattaccc
    17401 tggccactga ttgatggtgt cggacagatt tacgggatgt atgtcattac ggaaatcagg
    17461 accacccgtc aggagctgga tcgctatggc atggcccgca aaattgagtt cactgtcagc
    17521 tttcagcgat gcaatgaaga tctgcgtgag cgtcttcagt cgggctcggt gggtgatctg
    17581 atggcaggat taaaaaacag cgcgtcgtcg gccctgtcgt cggcgtcctc tgcgctgtct
    17641 ggtctcatgt tgtgaaggtc actgttcagg cttcggcttc ttttccccag ccaaccttat
    17701 agtccagaat cgccgccgta tcgttcaggg cggcgatctc ttttttcatg ttacgctggc
    17761 gaatgtgaat ttccatccct ttggcaaaca gggcttttgc ggctgcatcg ctgagggcaa
    17821 tcaactcatc agcagaaaca ggaacatcgt tgttctgtgc atcagtccag aaaaaatcca
    17881 gcggcaggat ccccgctttt gcggcagcaa cggaaggctc aaggcgggcc tgcgttgact
    17941 tcccgtagtc ccatttcctg ccgttatgtt caaaggtgta actcgccgct tccatcgcat
    18001 tacgccaggc gttgatttcg tcgtttttca ggcgtctggc gttttccctg tcagtgtcgt
    18061 tttcctggta ttcagataca gggccgtgat ttcctgcttt cagttcgccc cagagttttt
    18121 tgccgtgtgg cgagttgtca tattcggttg ccgtataggg cagccagctc tcctcccctt
    18181 caaactggat ctcacagtcg atagtgccgt cacggttata agcggcattt cttgctgatt
    18241 tgattgtgtg catgagtctc tccgtcaggc aatgcgctca aatattccga ttcctgcgcc
    18301 tgcataaatg tcagcgcagg cccgccacgt gcccggtggc ctgcggtttg tgttccagtt
    18361 ttgcatggat tcacgggggc cgaagttgcc ttcggaatga gtcaggctta tcagtctgag
    18421 tttactgccc gggcgggtgt ttccggccgt tatatcaatt ttggctggcc actccccttc
    18481 ggcatccgtt gccgccagaa taaagctgcc cacatcccag agtgtccgct gtgtttttgc
    18541 ctgtgcagcc tccagtgctt gccgtgcttt ctgcccggcg ttttctgctg ccgtcatggc
    18601 cgtcttcaca gcttttggtg tggcagccag cacttcactg tcactgtccg ttgcgctgct
    18661 tagctgaaca atacctcgct ctgtcaggga ggcgtcgccg gtctgcgtga gggggagtat
    18721 ggcaacctcc ttccattcag accatggccc gttgccggtg aagttacctg tcactgtccg
    18781 gataaatttt cgtggtggat tccaggcggt gtattcctgc tggcagaatc cccatgcgcc
    18841 catcctgatg gataatgatc cggctgatgt taccgggtag tgatgtgctg tagtggcgcc
    18901 gttgttgttg ggctgatagt agtcaccatg atgttcaggt ctccccagtg tatccaggtc
    18961 cacgctgagg gcggctgttg gtcttgttac tgccagcgcc ttaagtgctt ttattgcttt
    19021 gggtgtggct gcccgggttt catcatcact gtcggtttca ctactgagct gagtaaatcc
    19081 cttactggtg gttgtggcat ccgggtgttt accgttttgt tcatgctgct ttatcatttc
    19141 tgccacgcca gcctgtgttg ccatggtggt tgatgagtcg gctgtcagat ttacagaggc
    19201 ttcgttgctc agcagaatca ccatgcggaa tacctgttct ctgcctgagc cctcatcaag
    19261 ctgcggtttg taaccttccg caacattacc cacggcgatg agtgtgcctt tgctgtcaaa
    19321 cagaccgatt tcgcggatcc agaatcctcc ttcattcgca ggaatgatca cttcggcggt
    19381 taaaaaactg gtgttttccg cgtcacggct gaccctgctg acctcgcccc gccagacctc
    19441 tttaacgaga gcggtttgcg tggccagtgg ttctgtgggt ttaccgttac cgtctccgac
    19501 ggcgatatgt gtcagcgtga tcttttctcc atccggtaac accgcagctg cgagggctgc
    19561 tgttccggca tttgtgataa tggttttgta ttttgcggcc atcattactc cttgtccggt
    19621 ctgatggtta ctgtgatgcc ttcataggtg actccacccg ggtggatgtc accactgact
    19681 gacagcgggc ttgtcagttc tgctgccgga tataccacga tggtttcccc ggtcagcgtg
    19741 gccccggtta tgcgggatat cccccgtgca acgtaggtca ttttcatggc ggcaaggtgt
    19801 cggcttaccg ggcgaacggt ggtaatcatt cgctccatct cctgccgggt ttcctctgtc
    19861 agtccgcgct ccgatatgtc aatatccagc ctgaatgacc ccggcggatc gttgttctcc
    19921 caccattctg taatactcag tatcgcgccg aagggctcca cagcccgacg catggcgctg
    19981 atggtgcctt tgtggcggtg aatatgccac gagtcacgga tgatctgacg acgtgcctct
    20041 tcactccatg tttcatccca gcggtcaacg gaaagcgccc aggcaagcca tggcagcaga
    20101 tgggctgggc aggtgtcggg gttccagagg gtgttgatat ggatcggcag ctctgacagg
    20161 cgctgagcgc cctgttccga atgatgcagg aaggagggcg aggatggcgg cagcagtcgg
    20221 ttattcatcg gtgccacctg cagagatgtt ccgggatgtg cagcgggctg actgggtgtt
    20281 actgatcacc acattctgcg ctggtttctt cagctcgact cgctgaacgc ccggtacgtg
    20341 tagcgttgcc ataatggcag agcgggccac atcacgtcct atggcattct gagttttcag
    20401 ccatgtgtca agtgcatttc tggcggcggc aagaatgggt tcggattccg ggcccggata
    20461 aaaatagagt acggcctcaa tatcgtaact gacgatggtc gcactctgta ccgtgagtct
    20521 gtcggccagg gggcgcacag tttcatcatt gagagcccgg ctgacggtcg cgattaattc
    20581 cggtgacgct gtgccgtctc cttccgttga gagaatggag acaatgaccg tcgcaggtga
    20641 cggactggtt gcccgtgcat cggcaacaag accgctggca ctgcgggcaa aatattcata
    20701 tgcagcagag gggcctgcga cgctgaaacc ttcaaatccg gattgtgccc gcagacggaa
    20761 cgcgctgtcg ctctccatga tggcagcttc tgtatcagta gcaggtttca gttcaaggcg
    20821 tttcgcattc acgatagcgg cgagattatc caggtctgtg gatacggcat ggctgagcat
    20881 gcaccccgcc gctgcatcgt tgatccgctg acggagcagc atttcgcgaa aggcgatggc
    20941 ctgagcaatg atgttgagtg gctctgattc cagcgcaatg gctgcgctga cggcactgcg
    21001 catgttttca gggaaggcat caatcataaa ggccttcacg tctttcagta tgctctcata
    21061 atccagtgat tcaatgatct gcggacgtgg taactgagac aggtcaattg tcggcattac
    21121 tccccctcag cggaatgctc atctgtacgg gctgcattgt gcgggtgagc acgcctgaga
    21181 tggccagcag agcgcgtccg tcaggcagcc aggcgatatc cacccggtta atggcgatcc
    21241 ggggctccca ctgtgcgagc gccataacca ccgcgctcat gcactgcaga cgcgtcacgt
    21301 cgttttgtgg cgcatcgagc agttcaggta acaggctgcc atactctctt cgcatgatgc
    21361 gactgccgcg cggggtggtc agaatattgt ttacagagtg gcggaggtgt tctgtgtcac
    21421 tgatgcatcc gctaccatgc gggttcatgc ctgtgcatac cggtgtcatt tcggcccccc
    21481 tgtcatgctt ccgccggact gaacaccgtt atgaacgtgc gtatgcacgg taacaccatt
    21541 tgagcgtaag tttccgttac tgtgtgtgat atcccctttc agggtgccac cgtcagtcag
    21601 ttcaaacgtc ctgaccttca gatgttttgt gcactctacg gccggtgtgt ccagggtaat
    21661 acgtgtttcc gcagagatct ctgcggtttt gattcctttc accgtcagcg cgcctttttc
    21721 tgcgtcatag cgtatctcgg caccgtcagg tgcaaggagt atcacttcat tctgttttgt
    21781 gccgggcgcc gggtgttctt cgctgtagag actgccagcc acaacggcca tttcaggact
    21841 gccgcccggg cagagcaaca gcacctgttc accgggggag gggggcagcc agattttaaa
    21901 cgccccggcg cgtgtgcagt tccagcggag ccagtccgtt gtcagtccgc cgctggtaac
    21961 gcgaatgcgc cagtttttca gatccacctc cgaaacgacg ccggtgcgga ccatgttttc
    22021 cagcagtcgc atgatttcag cattcaacgg gtgtttctcc acaggttgtt gataatgatt
    22081 tccgtcactt tctgtctgtc ggcttcactg aatcccagca gttcgcgttg cggatagcgg
    22141 acggcattac cggcagagac cagtgacact tcgccgaact gatgaacgcg ggcaatgcgg
    22201 gctgcgcggc cttcaaatcc cacgctgacg ccgccgggat cggtttttac cttcagaaag
    22261 cggtttgtcc gaagctgctc aaacatgggt ggacgcttaa tggcgctctg cgttcgcctg
    22321 cgggtgtcaa tttccaggta ttcttcaata tcggctttgt ggaacgagcg tattccccgg
    22381 cgctccgtgt cgtaaccggt gatcatgggg ccgtaccggc cggttgttgt ccgccagttt
    22441 ttcagtcgtc tcacctgagt gccgttccag atgaacacca cacctgcctg gacacggaat
    22501 acacgttttt tacgggcggg ccaccggtta ccgtccggtg attgttgcga acgtatacgc
    22561 ttttgttgcg cggcgcggac agcctgaccg acagcacgtg ctgttttgtt tataccggca
    22621 gcggacagac cactgaggat ctcctgaaag agaccgtcca gtctgtgcag gtcatccgga
    22681 gccatcattc tgaccccatg tgacatcttc gagggtgacg cgccatgctt ccagggcccg
    22741 gctgacaggc ggctctccga tgtgttctgc gtgaacagtg ccatcatcat tacggcggat
    22801 aatcacccgc tcccataccg ggatttccag caggatatcg gtgctgtcgt cgtcattgat
    22861 ggcaacagaa aatttgatat ccctgttttt ctccggatta agcaggagat caggctgatt
    22921 gcggtacagc cagcgcatca gcggcaccat cagttcatcg agatcctgcg tgaagtccat
    22981 gacaaagatc ccaagcgtgt aacggtaaag atatgagggg gattcgccgg tcgtctcaat
    23041 gcctccgtta ttcacataca cagtgaattt ctccgggttg gcgcgacacc atgggcaggc
    23101 gtcagtcatg gcgtcgcgca gggtgttaat tttcagcatg ccccctcctg tgtgttatgg
    23161 catgttctgg ttgttctgtt gtgcagactt ctgactgatt ttttcagcgg cgcgctcgtc
    23221 aatttttcgc tgaaaataat ttctgataac actgtaaccg ccgctgatca gatacagcgc
    23281 gcagacggca gtacagaaat acagcatgat ctgatgaatg aataacataa tcacctcaca
    23341 caaatgattg gcataacgat gtctggcgtt ttaaaaaaaa tgaataacaa taataaacac
    23401 tctccataat tcctcttgcg ctcccgtcag gtatcttctc ctctaagtct tgcctgccgg
    23461 gagcctcttt tttcttatgc ggcgttgctg ttattgatat ccaccgtcac atactcaatc
    23521 agcacggggt aggtgtcctg gttactggtg atatccgtga tcagcagttt gtcgttatcc
    23581 agtcctgccg ccgggcattt cgccagatta aaggccgcct gggtctgacc gttgatgacc
    23641 ggtgtcacgc tgatgctgtt atttccggca aaccggaaca ccacgttgtg ccagttatga
    23701 ttaaatgccc cgaaggttcc cagttttgca ttctgttgtt tgtgctgcat cagattcagg
    23761 tttgcgctgt cgctctggat gaagaaggct gccagcatat tgcttcctgt gcttccctcc
    23821 ggaacaacgt tttcaggtag ctgagaaacc ggccagtaca gagccatggc gaagcggttt
    23881 gctgccagct gaacaccttc cgggattttg aaacgaaagg caatttcccc gcctttctga
    23941 agtaattcag ctgctttact tgcaggcaca tcgtgggcga ttttccaggc tgtttgttgc
    24001 ccctggactt tttccactcg catggcttta ccgctctcag cagcagagtc atttacgacg
    24061 gaggcttttc catctgcccc tgtccagccc tgttctttca gtttcccttc ctgtgcgtct
    24121 gcgcgataag cgacggccgt tgttcttgcc ggtgatgact gcgtacctgc gcccggctga
    24181 ggtgcaggtg tggagggctg cgggctttcc gtggtggtgc tgtctgaacc ggccgtggcg
    24241 ggaggtactg cgaccacgct gaacggtgcg ggtgcacctt tcaggaaggc gacggtcttc
    24301 ccggcatgca tcaggatcgc cgttgccagg cggtcagaaa taatgccgcg gcgggcccat
    24361 gaactgaaat gcgtggcgcg atctgaagag gtccagtttc cgttactacg ggatgcagca
    24421 ccgtaataac cggcttctgc caggtcagga tcttcggcag gcaggtttgt tggcgtgtta
    24481 gcacctttct catcggtcat caggggcaca aaataaattt tttgcgcttc tttgttctga
    24541 tatgcaccgt aaaccaccgg gtactgagag gtatattgtt gtttccagat ataggtggta
    24601 tcgccgcaaa tccagggaac aagtgatgcg ttgcctccgg tgcactgtgc agcatatgcg
    24661 ctgagatcgg cgcggaactg attcaccatg ttcaggaata attgcgggtg ctgtgcgtat
    24721 gttgctgatt tcagatcaaa ctctcccagc atccagacca cggataacag gacgtttttg
    24781 gggtttttat ccagcgcggc ttttgtacgg gtaagcagat cgttatacag cggtttgccg
    24841 acgccccaca gggatgattc ggccgttgct cctgaagatt cactgaatgc cccctctgcg
    24901 ccggaagtaa acgctgaccc tccacgacag caaggtacca ggaggatccc ggcatttttc
    24961 gggataaacg ccagtaattt tttggcaata tgtaaaccct gtccgacgca gccatactga
    25021 ccttttgaca gatcggcgtt cggatgatta aggctgctca tatcctgtac atcatggaga
    25081 cagtgatctg ccggaataat atcgttatag gtacatgcag cgccgcccgg tgtcaccgta
    25141 ctgcgacgcg ctaactgttt aattcgtgga tcaggactgt cgaatgtatc cggtaatggc
    25201 agcccctcac cgtatgccat gccattggac tgaccagcca gtggcaccac ataataaaac
    25261 tctggctcag ttgtgtgtcc agtgttgtat ccgtcggtag tgcccggtac gggtttttcc
    25321 tcgccttctg gtaccgggat ggctgatggc aggtctctgt cagtgaccag tgcctgcatc
    25381 aggtaaaatg gtgctcccgg ctctgatgct gagacaggtc ccccaagaag tccccatcct
    25441 tccctgagca tgggcgcaag tttttcggca agttccttgt tcgtcattgc cgtcacaagc
    25501 tgataatgtt tcggtaccat agtgactccg ttatttttca tgagtgttca ttcgtgctgt
    25561 ccgctgccgc tccagcccgg caatggcggc tttatcggca ttacaactgt ccagggcatc
    25621 taccagtcgg tctccccagt cagccacatc agcccatatc atgtgttgcg gcagcaccgg
    25681 taccggcgtc tgtctggtca gactttccgg cacgctcccg caaatgacgc cggttttcag
    25741 cggcgctggc gtgttgttgc aggctgtcag caacatcagc agggggagaa gtgcgtgcgc
    25801 actcatccgc ctgtaagttt ttccgcatcg tttcacgtcg ttcttcccct ctggctgcac
    25861 gctcctgatc ttcgcgccgg atagcggcca gcgctgactg tgtctccgcc gtggccttaa
    25921 gcaactcccg gacggtcgta ttgctctctt tcacggcttt ttccagcgct tcataacggg
    25981 tgctctcctg acctgagcga taggcccgga tgaccaccat tgcctgcagc gaaatggtca
    26041 gcagcagcgc acaaaggata atgttcagtt tcatggctgc gctcccgcgt ctttcaggca
    26101 ccagttctta aaatcattcc ggcggttaac cagtccctgc gtgcgcgttc cgccactgtt
    26161 cacaaaatct gtcagccggt aacacatgag gcgccattgc cgtgcctgcg cgtgtttcca
    26221 gatggtggtg cgttgcggcg tgccgttttt gtttttaaac cacatcagat tccggcagcc
    26281 caggttaaag gcggcgtccg tcatggcttc gaatgcagac tgcggcatct gctgccccct
    26341 gaaattctgt gtgatgcagt tctcagcgcg gcgcatatcg ttgatccacc gcccggccac
    26401 ttcctcctgt gtgtacaggc ggttttccac gttaccggtg gatccgcatc cgaccgtacc
    26461 gacccccacc agatcacggt agggtcgggc ccggcactcc tcccatgtgg caattttgat
    26521 ttgtgcctcg cgggaggttc tcagttctcc gggagccagg gagatcccca gcgccacaat
    26581 ggcggccacg gcgtaacgct taggtccggc catcgtgctc atcctgcttc agaagcatca
    26641 ttgcccggcg ctctgcgtcg ttcagctccc ttccttctga ctgcataagg attttttcaa
    26701 tcagttcgtt acgccttgcc tgggcgatct ccatcctgcg gcggtgaagc caggcacgaa
    26761 gcagtgacag cgtccccagc acaaaaccgg ccacggcaat tttttcgctc agcgtcatca
    26821 cgccgatacc ggctgtcgtc actgacgccc agaacgtgat gtgatcacaa agccgctgaa
    26881 acattctcac ccccacaact ggatcatttc acgccgggtc tgcccctgca gctccggcag
    26941 ctccacttcc tgtccggcat gcagaaacat gacggctgaa atccccggat tggcggccag
    27001 taccgcctca gtcaccccct gcgtggtgcc gtaatggcgc tgacagagta aatcgacggt
    27061 gtcaccgtcc agagccatta ctttcatcag accagctccg cataaatgcg ggtgacaccg
    27121 cgtatgtctg atattgccca gcgggcatcc cgccacaaat cctcaacctg catgtccagc
    27181 gcgtcagccc tgcggtcgcc tttatcggtg gtatccacat cccgggtgcg ctccagtaca
    27241 aacgcgcggg tgagtgcgaa gacggccctg cggtagcggt ggacgtgtac actctctccg
    27301 ttcacctgcg tggccggaac gtcagccagt gaggccgcgc cttcagccat ccgctcacgt
    27361 ttccagagct ccagttcggc gatcacatgg gcgaccgctt ccgttgctgc atgtaacaga
    27421 cgggttgttg ttgtcctccc cggaatgcgg gaagccaggc gaatttcatg cagtgagatc
    27481 gccggccaga agtcacccgc gctcaccatg gcgtcgccgt catcggtgtc ggtggtatct
    27541 gtgtcagcgc tgttcacgcg gggtctggct atcagactca tgcctgtctc cgtaaaaatc
    27601 aggcggtggg caaccggtaa aaagaacgcg aagcctgttc agatcaccgg ttgcgccgcc
    27661 tgccgacgag gtgcggttcg ttaagtcagt ttgttttgcg cgcggctttt ttctggctgg
    27721 cagcgctttt cttttttgcg ctgctctttt tcccgccggt ggtggttttc ctgcccgttg
    27781 tggatgcagg caccacctga tttccgtctg ccagtttcct gaccgcgcgc tccagcgtgg
    27841 taatttcacg cttcactccc gaagccggat tgatccgcat ggcttcacgt aacaggctca
    27901 gcgacagacg cagatcatcg ggagaggagg aggcgcggcg ggaaaatgcg cgtgccttgt
    27961 gcagttttga cctgacctgg tccggcatat cctgctcact gaccaggtgg ttcagctcat
    28021 caagcatggc ggtgtacaca gacaaatccg cctcgctgtc cgttgtggcc tgcgtcagaa
    28081 cggcgtcagt aatctcttca gccagtaccg tggcggcgtc acggccaaag ttatccggca
    28141 gcgccagacc atggcggatc acgtactccc cgatacgcag tgccagcggg tagtcacggc
    28201 agtcaatggc ccacaccatc atggtggtga tgacctcatc ctggcgtccg ctgtctcctg
    28261 ccagggtgcc ttcaatccac ccttcaaagt caggcaacag atgtttttta agctctgccc
    28321 tggcgacgtt tgactgaata cccttcagct gcatctgtgc cagccttaac tggtgaagga
    28381 cctgttcatg tgcggttctt tcccgttgtg tttcccgggc gttcatggtc acccggttgc
    28441 gggccaccgc ctgttgcaca tagcgttgtg ccggtgtcag catttctcag ctcctgttaa
    28501 cgggcgcggc gcgcccgtcc gggttatgct tcgggttgcg gctgtccgct ttcggcgttg
    28561 ttgtccggat cttcacctgc gaaggtgatc ccctcaatca gcgcgcattt tccgtagtct
    28621 tccaccacgt agcagtcatt catggactgg taagttgcga tacggttgta ttccggctcc
    28681 tcgcgaatga aacggcgcag tgagcctttc tggaagtaga tcgacaggtt tttcagcgac
    28741 gtgatgagca tggcgtcttt cgggaagtac ggcgcaagat acgtctgaag accaccgacg
    28801 gtacgggacg ccacaatcag ctgcccggca acaagctcag tgttcgggtt ggtggtgctg
    28861 atggcgttaa tcatgggaag acgcagtgta ttgaacagat cccgccccat cagtacgacc
    28921 agatccggtg cgtccttatg ccattcatcc agcacggatg agcggatgtc gtgcaccatg
    28981 gcatccgggt taccatagtc accttttgcc accagtttgt tttcatcatc gcgacgggtc
    29041 agggtcatcc ctttaaccac gcgggtggca gccttgtcgc ggatgtgttt cagccagccc
    29101 gggttgacgt cctgcaggag agggttggtg gcacgatcgg aaatcagcgc gtgtgactcc
    29161 ccgttgaaac caatcatgat tcgatccagg gctatctggc gggtgatttg cgcgctcagc
    29221 agttgctgaa agtttttctg gctgctccat gcgtcaatct gtgcgtagct gatgaaggta
    29281 tcgtagttca cctgctcgca gcggtaacgg cgtgcagcca gatcagcaac tgatgcagga
    29341 ttgcggcgtt tcactccgtc gctcgttgcg ttcgtgctgg caatggggcc gctggtgtca
    29401 atcaggactt tttcaccttc ctggtcatcc acaccgataa aattgatttc ctgtaacagt
    29461 ccggcgcttt ccttgatggc gttctccatc ttctgctgaa tggatggatc cacgctgaag
    29521 gttttaccca ggcgctctgt tgtgatacca gccagcgatg cctgctgaat catatagcgt
    29581 tccagactgt cacgtccttc acgggaaagg gataaacggg ccataaatta ctcttctcct
    29641 gtcagaattc agccagtgtc tggtcagtgc cgccagcgga agtaaaacgc tgctgtggat
    29701 cggcatcctg gtttctcagt tgcgctttca gtgctgcaag ctcgtgactg agctcatcca
    29761 gcgccgtttt gtcgatggca tgtttctgct ccagagtgct gaaacggtcg ataagatctg
    29821 catgatcgga tgcgatgttt tccacaacgt cgcgcagctg gctgaactgc tcgccatccg
    29881 ctgtgcgtgt tttgctgatg agtgtcatga cgcggctgaa ccactgacgg ctctcttccg
    29941 ttcgctgttc tgtggtggta agcatctctg cttccatggc gacggtgaac atggggggtt
    30001 ctgttgtgca cttactgaac tgcatgatgc tggcgcgttg ctgtgcggca aactccaggc
    30061 gttcagtgcc cagacttgct ggcgtgtcgg tcattgccag acccacaatg taaggcccct
    30121 tgacggcgtc cagctcaggc aacagctcaa tgctcgaata cacttttttc ccgtcagcaa
    30181 tcatgttttt catgcggtcg gtgggttcaa tttcggcata taacgctgta cgccctgcca
    30241 gtttcccttc ggtgatttca tcagttgaca gagcgatcag atcacccact gcgcagagtt
    30301 cactggatgg ccacatcgaa aggacgtgct caatgttcac ccgggaggcg taaacggacg
    30361 ggtcatagtt ttctgcggcc tgacgcagca tttcaccaga aatttcacgt ccgtcgatgg
    30421 ttgaaccgga aacggcaacg cgaaattttt tgcgctctgg cgtgtttttg cttcccatgg
    30481 aaattctcca taacctggtc gtttttccca tgatggcggc agataacggt gtgtctcaag
    30541 gtgctgaagt tgtatggcga cggggacaac acgggcaggt atcaggcggt attgctgagg
    30601 cttaatcttg ccctgttaat gtcagggggt gcaatgatcc aggatgtctt tattcgtatg
    30661 cgggcgaaac agctttactg gcaggggtat accccggcgg atatcgctca gctgatggga
    30721 ataaaccaga acacgattta ttcgtggaaa aaacgcgatg agtgggatgc aactcccccg
    30781 atccagcggg tcacccagtc cattgatgca cgcctgatac agctcaccgc caaaccggat
    30841 aaaagcggcg gtgactttaa ggaaattgac ctgctgacgc gtcagctgaa aaaactgaac
    30901 gaggggcagg gtgaacaggc tgtttccggt aagaagcccc gcaggcgcaa actgaaaaat
    30961 cactttacgg aagagcaaat catcgccctg cgtgaaagaa ccctccgaac actggcctgc
    31021 catcagcgtg aatggtatga aaaccgccac caccgtaatc gtatgatcct gaagtcgcgt
    31081 cagattggtg cgacctggta ttttgcgcgc gaggctctcc ttgatgcact gcgtgatgat
    31141 gtctccgcag ggtaccagcg caaccagata tttttgtccg cgtcccgtcg tcaggcatat
    31201 cagttcaaac actttattca gcaggcggcg ctggaagttg atgtcgaact gaaagggggg
    31261 gacaagattg ttttgtctaa cggtgcagag cttcacttcc ttggcacatc ggcagcaacc
    31321 gctcagtcgt ataccggaca ccttaaattt gatgaatttt tctgggtcag taactttgtg
    31381 gaactcagga aggttgccgg ggcaatggcg acactggaag ggcttacgcg aacctacttt
    31441 tcaacaccgt caggtgaaac acatgaggcg tatcagttct ggacggggga ccgctggaac
    31501 agcgaacagc cacgctcaaa acgcagtgag tttgatgttt cacatgcgca tctgaagggg
    31561 ggagtgctct ttcctgacga aacatggcgg cagattgtca ctattgagga tgctgtcgct
    31621 aacggatggc ggtacaccaa tattggtgtt attcgcggcg aaaatacaga aagtgaattt
    31681 cgtaatctgt acatgtgcga atttgtccgt gatggtgagg cagccttcag cctgagtgcg
    31741 ctcaccggct gcggtgttga cggctatgac gaatggcctg actggaaacc ttttgctgcc
    31801 cgccctatgg gggtgaggga ggtctggctt gggtatgacg ccaacggcag cagcgggaag
    31861 ggggactgcg gggcactgtc ggtgtgcgtg ccgccccttg ttgccggtgg gcgttttcgt
    31921 accgtggaga cggtcagggt gcagggtatg gagtttgaac agcaggccgc actgattgag
    31981 gaatacaccc aaaaatataa cgtgaaacat atcggcattg atgtgaccgg cgggaacggg
    32041 gagggggttt atcagattgt gaaaaagttt ttcccgatgg ccgtaccgta caacttcaat
    32101 atcgcctcca agcgggcact ggtcatgaaa atgcttcagg tggtgcgtgc cggacgctgg
    32161 gagtatgacc gcagtgaacg tgacctgatc caggcattcg catcggtgcg aaaaatacag
    32221 acacccggcg gctatatcac ctatgacacc gaccgctccc gcggtgtgag tcatggggat
    32281 cttgcctggg caaccatgct tgctgttatc aatgagccaa ttggtgcgga gcgtgaggat
    32341 aatcgcggct ttgcaatgga gttctgaaca caatgagtaa acgcaaaaaa tacaccggaa
    32401 agcgggggca ggattcgcta cacgatgcac tgaaatcaac gcctgcactc agcgcattca
    32461 cctttgatgg gccctatgaa gtcagccgca tggatctcct ggataacatg tcatgtgcag
    32521 ataacgggcg ctactttgat acccccatgg actggaacgc tatcgcccgt gcatcccgtc
    32581 gtgcgtcctg gcatcagtcg gctttgtact tcaagcgcaa cgccctgaac gggtgctttg
    32641 ttcctcaccc tctgctgtcc cgccaggcgt tttcagcctt tgcgctcgac tggtttgtgt
    32701 ttggtaatgc ctatcttgaa gtgcggcgta acaggctggg ggaaccactc ctgcttcgcc
    32761 cggcactggc aaaatataca cgcaggggaa gtgacctgga tacctactgg tatctcaacg
    32821 acgacggtac ggagttcagt ttcaggaaag ggactgtgtg ccacgtgctt aatcctgata
    32881 ttaatcagga gatctacggg atgccggaat atatcggcgg gttgctgtct gtcagcctgt
    32941 caaactcagc ggatacattc cgcaaactct actacgataa cggctcgcat gccgggtgta
    33001 ttatctatgt aggcacacct caggcgaatg cggaaagtgt cgaggccatt aaaaaaacgc
    33061 ttacggattc ccgtggtcgc ggggcgttta aaaacctgtt tctacatgcg ccgggtggtg
    33121 gcaaggatgg tgtgcaaata ctgccgttcc agcagataac cgcaaaggat gaattcctga
    33181 acatcaaggg gagcgcccgc gacgatatcc ttgctgccca tcgtgtacca cctcaactga
    33241 tgggggcaat gcctgacggg aatgcggcgt ttggtgacgt tgaaaaagcg gcgagagtgt
    33301 ttttcatcaa cgagttgcaa cctgtcatgg aggccatgaa gcatgtcaat gaatggcttg
    33361 gcgttgaggt gatgcgcttt aacccctatg ccctgcttct ggataacgcc agctgacgcg
    33421 cttcgcttgt ctgctgcttc gccggggcat aaaaaattta tgccccgact ctccagctct
    33481 cttaacggaa tgcggatggc gttcttcttt gcagcttgct gataatatgc atcttcatgc
    33541 tgtttactat cggcagccat tccctgtcac cgtctccgtt gatgaccacg cgaatggagg
    33601 tatcatcttc cacgcgaacc cgaagagata caccgtcttt tccgacggaa tgtaactcca
    33661 ttgccaggac tatgtttacc gactctcctt catcgtgtga gtcaacccat gttcttacag
    33721 ccatccggaa tatgttatct gtgtcggttg caatcatatt taccggaaat ggcctgaagt
    33781 cccgatcgtt tctgcacgga aaccccgtat caacgtaacg atgatgctcg ccgtctttgt
    33841 cttcccatgt ctctgaaggc aattccagcc actgctcata aaaagcaacc agcttttcag
    33901 cctgattgcg cagaatccct gtaacctccc gcctcgcgtc ccggctcttt ttgtttgcca
    33961 ggtacaaatc gcgaaaatgg ctcatgtcgc tctcctttgg tctgtttatg ccagcctgta
    34021 ttctgcgctc acctcatatt gtgcgcaagt tttcacccgt gaatgactga tactttaaat
    34081 tctattaact ttatgatttc aatgattttt gtctctcttt tttatcccgt tcgtcacgtc
    34141 cgtttccctc ccgatctccg acctcgccag acgcaatcag acgcattctg acggcctgtt
    34201 tccctgacca tgttttgatg tggtttttca aaactgactt tactgcgtgc gtatcacgcg
    34261 caatacgacc ccaaataaag caacccgcct tccatccctc aattcgggcg cccttccctc
    34321 gtcgcctgcg cgcagcgatc gcctcgtttt tcgtgcaccg tgcaaagtgg cttcagccag
    34381 cgccgttact gggctgaaag ggtaaaaaac acataagaaa aattgtgcaa tttcgtgcac
    34441 ttttgtgcgg ttttccaggt ggtgtttatg cgggaatcgc aggctaaaaa aagcatgagc
    34501 ggggattgcc cccgcgatat tcatgccgcc cgatgagctt cacctactga cgggtctatt
    34561 atgatttcaa aatccgtgaa caacgctttc ggctgtacgc agttaccttg tttttccagg
    34621 ctgacaaagc cgtatcgact cagcattttt aacgttgctg acaaattact taactgcctt
    34681 cctgaaagac tggcaagttc tgtcattgtc tgcggttttt ccgcatccat gatacgcaga
    34741 agcgcgatat tttcgttgct cagcgcctga cccagagcgt tcagagatga aaaccagact
    34801 cgcggttcgt caggttctgg ttttagtttc cctttggcta tatccagcat tctctggcga
    34861 aggactttct ccgggataat gccgatacgg gctttcattt cttccccctt tgctgttcag
    34921 caatcactct gtctacatgg ttaaagaaat ccagtaaaag ctgttccgca gaagtgaatt
    34981 catatggata gcctttatcg gacgatgttc tgtgaatatg gtcataaacg gtaatgcgcc
    35041 cggtaaattt ccctttcttt ggtggcttta ttccgtgagc gttatccatt ccaaaaaccc
    35101 gggagttata aggatcgtgc aatgtcaggt tgtagcgtat tccatgtggt ctgaactggc
    35161 ttgggctaac cctgtatgct tcaatcttcc accagtaacc attatccatg tggtgctggt
    35221 atccatgcat atccagaagg gtgttcagcc catgatcatt gtttttcata actgattcgt
    35281 atcaccttac ttatgaccaa atcataacat cgtttttttc tttatcaagg tgggttattg
    35341 tgctgttctt gtggttatcg acgttaactt atgtacatat ttgaattcgt agtggttaat
    35401 atgaaaaaag tgtctgacga atttgacggc ttctgaatac gtaaaactgt gtcaggcgga
    35461 gtatgtttct ccgccttttt gtttgtgcca gcccggcgtc tcacatcatt actcacatat
    35521 tgatcatatt gcggttcttg agctgataat gctttgtgcc agcaactgcc agccttcttt
    35581 catctgtccg tatttgtcct gatatctgtt tacctgcaag ttcccgtgca cctgtaccag
    35641 gtcgcctttt ttgtgttttg ccagttcttc gtgctgcttc ccgaatgcga taaggtaaag
    35701 ccacattgtg gttgactgtt ctgcgccatg gccgggcagt gatatcgcca gtcgtgcagc
    35761 tgttacatcg gtgcctctgg atgattttct tgtcgtcggg tcttccgcca gtcgtccccg
    35821 tgttgcaatc tgcgttgtca tatttgtctc cgttggtggt ttagttattg gactgatatg
    35881 actggcagat ctccgccagc gccgtagata aacagaattc gcttcatgac ttcgctgcgc
    35941 ttgcagactt cgatgactga tttttcctgt tgccattcag cccgttgcgc cagctccagt
    36001 cgttcctctt ccgttggctt ccggtaatgc gttatcttgt ccgtgtaccc gtccacaacc
    36061 agcaggcctt cccgtaccat ttttccggcg gcctgattga tgacggttct tttcacgccc
    36121 agtttttcta ccagttccgg tgcgctgaag cgtgcgtgcg tttccaggta gcgtaaaatg
    36181 atttcctttc cggtcattcc tggggtcttc atttggtggc tccttcgttg ttccggcgct
    36241 cccggagttc ggtcagtgct gccagtaaat tttcccacca tgtttcttcc acgaccggat
    36301 tttggcgtga aagcagctgt tgcgccgact caatcatgct gttcaggtcc ctgtcgctga
    36361 tcgccctggt gctgttaatg gtgtgttgct gtctggtgct catgatgtct gtggttctcc
    36421 tgttacttgc ggtgctgctt tctggcggct gaatgtacat aacttgctat ggccgccttg
    36481 cggttaatgc gcttcatcgc cctggctaaa tcttcgttgt gtttcctgat atccttttcg
    36541 tgatatgcct tcatgatcct cccggctggc gtcagtggtt ctggtgctcc cgtgttcctc
    36601 agtctcaggg ctgcgataaa gtcgccgctg tctccccatg gtctggcgtt caggatgtcg
    36661 gcagtcatac tttcggtact cctgtctgga cgggctatgg tgatttccag ctggtcacct
    36721 ggctgcggtc ttcttatcag tcctctcagc gtcagcgccg gtaatctctt ggtcatgacg
    36781 acgctgaact ctcgttgctg aagcgtgcag ttgaatcgcg tcattcatcc cccttcagct
    36841 gtgctacttc cttcaggctt ccggtgcttt catcccagtg aacaatccgc ccgtcaggca
    36901 ggcgaacgcg cgccccgcga tacacggaaa gcgccgcact actgtgtaac tcctcgcccg
    36961 ttgtactcag agtactggcg atcaacctgg tggtcatatc cggttttgcg agtgccgcac
    37021 cggcatatac cacgccttca ggtgcgggga acccgccgct gtttgcggtt tttgtggatt
    37081 tctggtcatt tttcagctgt tgggtgagct gttttctctg tactggagtc agtgacctta
    37141 accactcacc cggatcacct tccggtacga ttagtttttg ttctttcctc ccttccggtg
    37201 gtggtgtttt gtccgtttcg ttgtttgttt tttcatcatg gcgacagtta ttgccacgag
    37261 tccaaggtgc ggcaaagccg cactcaacgt caaaaccctc gtccgtgcgg tcttcgtttt
    37321 ccggcttacg tctgaccaat ttccaggtgt ccgggtgggt gcatatggtg tagcgctccc
    37381 cgagtgacgg ggcgcggacg ccgtaaatac gttgcgggaa ttcgccgta
//
LOCUS       DAZGBC010000028        36464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGBC010000028 DAZGBC010000000
VERSION     DAZGBC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.028"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..635)
                     /locus_tag="ACHK1J_001451"
     CDS             complement(<1..635)
                     /locus_tag="ACHK1J_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7910749.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALERACD"
     gene            complement(632..1048)
                     /locus_tag="ACHK1J_001452"
     CDS             complement(632..1048)
                     /locus_tag="ACHK1J_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910750.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVDPRLASCLNDWHDYYRRACKRAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSV
                     AMKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            complement(1117..1344)
                     /locus_tag="ACHK1J_001453"
     CDS             complement(1117..1344)
                     /locus_tag="ACHK1J_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO7910751.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVSFIMQSNVLHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            complement(1275..1658)
                     /locus_tag="ACHK1J_001454"
                     /pseudo
     CDS             complement(1275..1658)
                     /locus_tag="ACHK1J_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1590..2192
                     /locus_tag="ACHK1J_001455"
     CDS             1590..2192
                     /locus_tag="ACHK1J_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910752.1"
                     /translation="MRCGKTVTRPGERWQAVFVLRADVVRMWRINITWQLVIVCIRGV
                     FLMSDKLPSDYDILFDLWYSYWLERITCVFHRRVSAALRISAFLVGVAAFVLCDFVVL
                     VLFSVVLFLLWQCMAASFERRAEKAEWQAVSYQRLISQRHVISKECMIARLSFVEESD
                     SPIMECMINLAWNKACEAVGSSSRNHVSISGRLLSRLCGF"
     gene            complement(2166..2516)
                     /locus_tag="ACHK1J_001456"
     CDS             complement(2166..2516)
                     /locus_tag="ACHK1J_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO7910753.1"
                     /translation="MAVIYIAGPMTGYKDDNRLEFCLADLKLRYAGNIVLNPAVLPDG
                     LSQQQYMSICIPMLMCADAIYLLDGWEESAGARAEYAMALKLNMPVSFPENRAAGETV
                     AQLLGKSKAAQATE"
     gene            complement(2507..2725)
                     /locus_tag="ACHK1J_001457"
     CDS             complement(2507..2725)
                     /locus_tag="ACHK1J_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910754.1"
                     /translation="MIQESTVIRFTANGRQYEVDESLIDQGMTRQDSRNPEMHHIRLI
                     NGSHFCATNMEEVRVLTATRNAGGKAWP"
     gene            complement(2722..2958)
                     /locus_tag="ACHK1J_001458"
     CDS             complement(2722..2958)
                     /locus_tag="ACHK1J_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910755.1"
                     /translation="MNSERNKALLRGLRHIAHAHEETAKVAAAVGKPKVVISSSISLG
                     ITMEAIRVIQDQESEIAALKKQLNEKSAGQRGQQ"
     gene            complement(2994..3974)
                     /locus_tag="ACHK1J_001459"
     CDS             complement(2994..3974)
                     /locus_tag="ACHK1J_001459"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO7910756.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKVHLPEADCLVEPFVGGASV
                     FMNTSYKRYILGDSNGALINFWRAARDNTDSLIDEAKTLFLNHNNRGDYLIIRETFNA
                     ENHAFLSSRTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGCYRKPYFPE
                     HEIRAFADKANSTRTLLIHADFKDTLNSASHLFGMGNTLCVYCDPPYLQFSGRDNFTA
                     YGKPFDEHEHVRLRAALDRLSQETGGMTSITISNSDTPETRRIYQGYRMNCIQAPRSV
                     GARTREPAPEVIATLRQCDACGHYGGGYCPDCGPVTGDATYCVMFTPAST"
     gene            complement(4207..4788)
                     /locus_tag="ACHK1J_001460"
     CDS             complement(4207..4788)
                     /locus_tag="ACHK1J_001460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7910757.1"
                     /translation="MNHLMIDTETLGNGPDAVIFAIGAVFFDPFTGKLGKQFEKFIDP
                     VDSERNGGTVSATTAVWWAGQSVEARACLRNAEGTELAAVTEFLAFISRNMHDESPGN
                     SLTIWCKGASFDFPILRSAIKRTTGEKSIPWCYWDERCMRPLIAMAESTGWRMPGRSG
                     KEAIAHTALGDAIYQAKVVSEIWQRFTTPFLNI"
     gene            complement(4785..5018)
                     /locus_tag="ACHK1J_001461"
     CDS             complement(4785..5018)
                     /locus_tag="ACHK1J_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910758.1"
                     /translation="MRTAESPGREITARPANHHAHISMAARKAERAHICELLRELTET
                     AKEKHTSPLETCSVIERLIAMLTSQDEPKEPVQ"
     gene            complement(5233..5358)
                     /locus_tag="ACHK1J_001462"
     CDS             complement(5233..5358)
                     /locus_tag="ACHK1J_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910759.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIHTLKDIELFFTGDKRRK"
     gene            complement(5355..5597)
                     /locus_tag="ACHK1J_001463"
     CDS             complement(5355..5597)
                     /locus_tag="ACHK1J_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO7910760.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVAA"
     gene            complement(5616..5951)
                     /locus_tag="ACHK1J_001464"
     CDS             complement(5616..5951)
                     /locus_tag="ACHK1J_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO7910761.1"
                     /translation="MKQQRNARINNNTERHVARQHFRKNSATVTTEIHATPDGHPVKQ
                     TGKHTWAIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTADRLISGL
                     RFQYPGSKH"
     gene            complement(5977..6171)
                     /locus_tag="ACHK1J_001465"
     CDS             complement(5977..6171)
                     /locus_tag="ACHK1J_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910762.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            6170..6607
                     /locus_tag="ACHK1J_001466"
     CDS             6170..6607
                     /locus_tag="ACHK1J_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910763.1"
                     /translation="MSIPSNLDVFNETVGKTFAFLYETFPRKVTIDICALTGVSELTF
                     EEDRSSRRQLVEALELRRDSIAWLVDAGYVSAKSSPPHYFSDAVLTAKGLELLKLDPE
                     SLKQSFGDKLVDAAKSGSMDAIKSTASSALTTGLSFAFKSLLG"
     gene            complement(6689..7114)
                     /locus_tag="ACHK1J_001467"
     CDS             complement(6689..7114)
                     /locus_tag="ACHK1J_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO7910764.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGLRNKRISKKAA"
     gene            7222..7521
                     /locus_tag="ACHK1J_001468"
     CDS             7222..7521
                     /locus_tag="ACHK1J_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7910765.1"
                     /translation="MSTNVGEKIRIMRKSEMLTQEKMAEITGLTVAALRQYEQGRNEP
                     NLESTKKLLKSPVFRKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            7588..8583
                     /locus_tag="ACHK1J_001469"
     CDS             7588..8583
                     /locus_tag="ACHK1J_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7910766.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            8873..9124
                     /gene="chpS"
                     /locus_tag="ACHK1J_001470"
     CDS             8873..9124
                     /gene="chpS"
                     /locus_tag="ACHK1J_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO7910767.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            9118..9468
                     /gene="chpB"
                     /locus_tag="ACHK1J_001471"
     CDS             9118..9468
                     /gene="chpB"
                     /locus_tag="ACHK1J_001471"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO7910768.1"
                     /translation="MVKKSEFERGDIVLVGFDPASSHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(9548..10078)
                     /gene="ppa"
                     /locus_tag="ACHK1J_001472"
     CDS             complement(9548..10078)
                     /gene="ppa"
                     /locus_tag="ACHK1J_001472"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO7910769.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            10388..11344
                     /gene="ytfQ"
                     /locus_tag="ACHK1J_001473"
     CDS             10388..11344
                     /gene="ytfQ"
                     /locus_tag="ACHK1J_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO7910770.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            11484..12986
                     /gene="ytfR"
                     /locus_tag="ACHK1J_001474"
     CDS             11484..12986
                     /gene="ytfR"
                     /locus_tag="ACHK1J_001474"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO7910771.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQPISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIEMLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            13000..14022
                     /gene="ytfT"
                     /locus_tag="ACHK1J_001475"
     CDS             13000..14022
                     /gene="ytfT"
                     /locus_tag="ACHK1J_001475"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO7910772.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            14009..14968
                     /gene="yjfF"
                     /locus_tag="ACHK1J_001476"
     CDS             14009..14968
                     /gene="yjfF"
                     /locus_tag="ACHK1J_001476"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO7910773.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLISEESIPINHPVYDTLSSLVWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQVGYALAGVGVELDAIASVVIGGTLFGVAIQGLIQTYINFDGTL
                     SSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(15001..15999)
                     /gene="fbp"
                     /locus_tag="ACHK1J_001477"
     CDS             complement(15001..15999)
                     /gene="fbp"
                     /locus_tag="ACHK1J_001477"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO7910774.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            16175..17548
                     /gene="mpl"
                     /locus_tag="ACHK1J_001478"
     CDS             16175..17548
                     /gene="mpl"
                     /locus_tag="ACHK1J_001478"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO7910775.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(17704..18255)
                     /gene="yjgA"
                     /locus_tag="ACHK1J_001479"
     CDS             complement(17704..18255)
                     /gene="yjgA"
                     /locus_tag="ACHK1J_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO7910776.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            18349..19701
                     /gene="pmbA"
                     /locus_tag="ACHK1J_001480"
     CDS             18349..19701
                     /gene="pmbA"
                     /locus_tag="ACHK1J_001480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO7910777.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            19885..20271
                     /gene="cybC"
                     /locus_tag="ACHK1J_001481"
     CDS             19885..20271
                     /gene="cybC"
                     /locus_tag="ACHK1J_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO7910778.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(20316..20780)
                     /gene="nrdG"
                     /locus_tag="ACHK1J_001482"
     CDS             complement(20316..20780)
                     /gene="nrdG"
                     /locus_tag="ACHK1J_001482"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO7910779.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(20970..23108)
                     /gene="nrdD"
                     /locus_tag="ACHK1J_001483"
     CDS             complement(20970..23108)
                     /gene="nrdD"
                     /locus_tag="ACHK1J_001483"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO7910780.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(23502..25157)
                     /gene="treC"
                     /locus_tag="ACHK1J_001484"
     CDS             complement(23502..25157)
                     /gene="treC"
                     /locus_tag="ACHK1J_001484"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO7910781.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPEQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(25207..26628)
                     /gene="treB"
                     /locus_tag="ACHK1J_001485"
     CDS             complement(25207..26628)
                     /gene="treB"
                     /locus_tag="ACHK1J_001485"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO7910782.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(26747..27694)
                     /gene="treR"
                     /locus_tag="ACHK1J_001486"
     CDS             complement(26747..27694)
                     /gene="treR"
                     /locus_tag="ACHK1J_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO7910783.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            27879..27932
                     /gene="mgtL"
                     /locus_tag="ACHK1J_001487"
     CDS             27879..27932
                     /gene="mgtL"
                     /locus_tag="ACHK1J_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO7910784.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            28073..30769
                     /gene="mgtA"
                     /locus_tag="ACHK1J_001488"
     CDS             28073..30769
                     /gene="mgtA"
                     /locus_tag="ACHK1J_001488"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO7910785.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(30975..31361)
                     /gene="ridA"
                     /locus_tag="ACHK1J_001489"
     CDS             complement(30975..31361)
                     /gene="ridA"
                     /locus_tag="ACHK1J_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO7910786.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(31434..31895)
                     /gene="pyrI"
                     /locus_tag="ACHK1J_001490"
     CDS             complement(31434..31895)
                     /gene="pyrI"
                     /locus_tag="ACHK1J_001490"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO7910787.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(31908..32843)
                     /gene="pyrB"
                     /locus_tag="ACHK1J_001491"
     CDS             complement(31908..32843)
                     /gene="pyrB"
                     /locus_tag="ACHK1J_001491"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO7910788.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(32847..32981)
                     /gene="pyrL"
                     /locus_tag="ACHK1J_001492"
     CDS             complement(32847..32981)
                     /gene="pyrL"
                     /locus_tag="ACHK1J_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO7910789.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(33262..33657)
                     /gene="yjgH"
                     /locus_tag="ACHK1J_001493"
     CDS             complement(33262..33657)
                     /gene="yjgH"
                     /locus_tag="ACHK1J_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7910790.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGS
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDNIIDITSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(33788..34501)
                     /gene="bdcA"
                     /locus_tag="ACHK1J_001494"
     CDS             complement(33788..34501)
                     /gene="bdcA"
                     /locus_tag="ACHK1J_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7910791.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDHL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            34572..35165
                     /gene="bdcR"
                     /locus_tag="ACHK1J_001495"
     CDS             34572..35165
                     /gene="bdcR"
                     /locus_tag="ACHK1J_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7910792.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            35310..35762
                     /gene="tabA"
                     /locus_tag="ACHK1J_001496"
     CDS             35310..35762
                     /gene="tabA"
                     /locus_tag="ACHK1J_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO7910793.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            35885..>36464
                     /gene="yjgL"
                     /locus_tag="ACHK1J_001497"
     CDS             35885..>36464
                     /gene="yjgL"
                     /locus_tag="ACHK1J_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO7910794.1"
                     /translation="MSKISDLNYSQHITSADNFKPKNEVLNTWCVGMNDFARIAGGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSSKVIIEISLSTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDDS
                     SLHLITNKVLACYQREANKEIARLLNNHQKLNN"
BASE COUNT         8462 a         9019 c         9726 g         9257 t
ORIGIN      
        1 tcatcacatg cgcgctccag tgcggtggaa aacgcggtgg cggtcagggt ggcaaggcgc
       61 tggatttcgt cacggctcag ctgcggcagc ataagcagtt catccagccg ttcgcgggcg
      121 gcggtggcct taaaggccgg ggtggcaaag cgttcattca cacgggcgat acgtttcagg
      181 cgggcacgca gcccgtgacg aacgaagtta tccgccgcat atgcgctgtt attcttaagc
      241 ccttcccggc gtgcgctctc ctgtttgcgg ctcaggcgcg ccaggtcacg gatgagcgga
      301 ccggcaagaa acggcggcag ggcgcgcagc tctgccatgg cttcagatac cagtgttggc
      361 ggattttcgt gtttgtgccg cgccagtttt tccagcctga tccgacgctc ttcgtcgtgg
      421 taaagatgcg aattaattcg cttcccttcg tctgtggttg tcaggaaggc gacggtttct
      481 tcatccagtc tggcgtcagt gcgaatatcc tcgttgatgt agctctgaat ggcctggcgt
      541 aatgcggttg gctgcgctct gccgaacgtt tcaggatcga taaccggtag cggggcgttc
      601 cacgggtacg ccagttgtgc attatggtcg ctcatgcgtc gcactccccg gcagtcgccg
      661 cctgaaacgc cgcgcggtac agcacgagaa ggcgctggta ttcaccgata aagtcttctg
      721 cggtgtatat gcctttcatg gccacgctgt gcgttgtcat ttccagaaca agcgtcatct
      781 ctgacgacga ccagctgatg gcggtgctgt cggttccgtt gatgtgaaat aaaccaaaac
      841 catcaccatc acctttcacg tccacgcggt aacggtcgtc aacggtgaag gagaaatccc
      901 cgtatgtggt gacgcgtctg gcggcccgtt tacaggcgcg gcggtaatag tcgtgccagt
      961 cgttcaggca ggaagccagc cggggatcca ctgcccacat ccagttaaaa aaatcctgaa
     1021 tatctgaaag tcccttaacg ttcttcatga gaacctccgg taacagacgt gcggaagcct
     1081 cccgcgcacg ggtgcgggat ggcttcagga tgttgtttag tggtggtgtt gctggttgat
     1141 aaggtcctgc agttctgcca ggtcttcggc cagatagctg aagactgccg cgtaataccc
     1201 ggcagcgata ccgtcgttaa gtcggtgtaa tacgtttgac tgcatgatga aggagacaga
     1261 tgcagcccga tggagtctgc ggttgatttc agtgcggatg tgaagacgct cagcgcgggc
     1321 gcgctgttgt ttgcggtttg ccatggtgtg gcctcgtacg tagtaagttc tgaaaactca
     1381 ccaacccgag gttccaagct cagggtggcg agacgtacag ggttggaact accggctacg
     1441 cacgaacccg gcccgaccga agtcggcccc gtacgccccg ccataattct gacgtgaaaa
     1501 agacgtggcg atacagtacg cacaaaaaaa ccgctggcgc ggttgtgcgc gtgcatagtc
     1561 ggcggggttc caatcccggc acctgtttta tgaggtgcgg gaaaactgta acccgaccag
     1621 gcgaacgctg gcaagcggtt tttgtgttgc gtgctgatgt ggttagaatg tggcgaatta
     1681 atatcacctg gcaactggtg attgtctgca tacgcggggt gtttctgatg agtgataagc
     1741 tcccttctga ttacgacatt ctttttgacc tgtggtacag ctactggctt gaacgtatta
     1801 catgtgtgtt tcaccgacgc gtttctgctg ctctgcgaat cagcgccttt ctggttggtg
     1861 ttgcggcgtt tgtcctgtgt gattttgttg tgttggtact cttcagtgtg gttctgtttc
     1921 tgctttggca gtgcatggct gccagttttg aacgcagggc tgaaaaggct gaatggcagg
     1981 cagtcagtta ccagcgcctt atcagtcagc gccatgtgat tagtaaggaa tgcatgattg
     2041 cgcgcctctc ctttgttgag gaaagtgaca gtcccatcat ggagtgcatg attaatcttg
     2101 cctggaataa agcctgtgag gcggttggca gctcttcgcg caatcatgtt tcgatctccg
     2161 gtcggttact cagtcgcctg tgcggctttt gatttgccaa gcagctgtgc gacagtttcg
     2221 ccggccgccc gattttccgg gaatgacacc ggcatgttca gcttgagtgc cattgcgtac
     2281 tcagcccgtg cgccagcgga ctcttcccat ccatccagta agtaaatggc atcggcacac
     2341 atgagcatcg gaatacaaat gctcatgtac tgctgctggc ttaatccgtc aggcagtacg
     2401 gcaggattaa gaacaatgtt tccggcgtac ctaagcttca ggtccgccag gcaaaactcc
     2461 agtctgttgt cgtctttgta tccggtcatg ggaccggcga tataaatcac ggccatgctt
     2521 tacctcccgc gttgcgtgtt gctgtcagca cgcgtacttc ttccatgttc gtggcgcaga
     2581 agtgagaacc gttgataagg cggatgtgat gcatctcagg attgcgtgaa tcctggcgtg
     2641 tcatgccctg gtcaatcagg ctttcgtcga cttcgtactg gcggccgttg gctgtgaacc
     2701 ggatgacggt tgactcctgg atcattgctg acccctttgt ccggcagatt tctcgttgag
     2761 ctgcttttta agggcggcga tttcactttc ctgatcctga atcacccgga ttgcttccat
     2821 ggtgatcccc agtgagatcg aggaagatat aacgaccttt ggttttccaa cggcagccgc
     2881 aactttggct gtttcctcgt gtgcatgggc gatgtgacgt aacccccgca aaagtgcttt
     2941 gtttctttcg ctattcattt cctgactcct gtgttactga ttcatctgtt tattcacgta
     3001 ctggcagggg taaacatcac gcagtaagtt gcatctcccg ttaccggtcc gcagtccggg
     3061 cagtagccac cgccatagtg accgcaggcg tcgcactgtc tcagggtggc gataacttcc
     3121 ggtgctggtt cgcgggttct tgcgccaacg ctgcgtggtg cctgtatgca gttcatccgg
     3181 taaccctgat aaatgcgtct ggtttccggt gtgtcggaat tggagatggt gatgctggtc
     3241 atgccgcccg tttcctgcga aaggcggtca agtgctgccc tcaggcgaac gtgctcgtgt
     3301 tcatcgaatg gttttccgta ggcggtgaaa ttgtcccggc cactgaattg caggtaaggg
     3361 ggatcgcagt acacgcaaag cgtgttgccc atgccgaaca ggtgactggc agagttcagc
     3421 gtatccttaa aatctgcgtg gataagcagc gttcgggtgc tgtttgcctt gtctgcaaat
     3481 gcgcggattt catgctcagg gaaatagggc ttgcggtagc agccgaaagg cacgttaaaa
     3541 tcgccttttc cgttcacccg gtacaggcca ttaaaacagt gccggttgag gtacaaaaac
     3601 atggcggcca gtcgcagttc acggcggtcg cttattcccg ttctgctact caggaaggca
     3661 tgattctctg cgttaaaggt ttcgcgaatg atgagatagt cacccctgtt gttgtggttc
     3721 aggaacaggg ttttcgcctc gtcgatcaga ctgtctgtgt tgtcccgtgc tgcccgccag
     3781 aaattaatca gcgcaccatt ggaatcaccc aggatgtagc gtttgtaact ggtgttcatg
     3841 aataccgatg cgccacccac gaacggttca accaggcagt cagcttctgg cagatgcacc
     3901 ttcagtgtgt tcatgatgcg gcttttgcca cccacccatt tcagcggtgt gccggataac
     3961 agttctttgc tcataacgcg gcctctttgt tataggtttc atgcgttaat aaatcccatg
     4021 acttcccgtt gttcttgctg agtaatcgcc agcgaaaacc gattcttatc accaggtagt
     4081 tgtggggctt tatgcgtgaa aaattccgca caccgttgcg gaataaacgc agcgatgcgc
     4141 gggcttttct ttccacgttc aggggggggc agcaacacac attcaggatg gcgaccatgc
     4201 ttcacctcat atgttcagga atggcgttgt gaagcgctgc cagatttctg acacgacctt
     4261 tgcctggtaa atggcatcgc ccagtgcagt gtgagcaata gcctcttttc ctgatcggcc
     4321 tggcattctc cagccggttg attcagccat ggcgataagc ggacgcatac agcgctcgtc
     4381 ccagtaacac cacggaatac ttttttctcc ggttgttcgc tttatggcgg atctcaggat
     4441 tgggaaatcg aaggatgcgc ccttgcacca tatggtcagt gaattgcccg ggctttcgtc
     4501 atgcatattg cgtgaaatga acgccagaaa ttcggtcact gctgccagct ctgttccctc
     4561 tgcgttgcgc aggcaggccc tggcctccac ggattgtccg gcccaccata cagcggttgt
     4621 tgcactgacg gttccgccgt tgcgttctga atccaccggg tcgatgaatt tctcaaactg
     4681 cttgcccagt tttccggtga acgggtcgaa aaacaccgcg ccgatcgcga atatgactgc
     4741 gtccggtccg ttacccagtg tttcggtgtc aatcatgaga tggttcattg tacgggctcc
     4801 ttcggttcgt cctggctggt gagcattgca atcagtcgtt caatgactga acaggtttcc
     4861 agcggggagg tgtgtttttc tttcgctgtc tcggtcagct cgcgcagtaa ttcgcagata
     4921 tgtgcacgct cggcttttct tgcggccatg ctgatatgcg catgatggtt agctggtctg
     4981 gcggttattt ctctgccggg tgattcggct gttctcattt gttgactcct aaaaaaaaga
     5041 caaagggaac ccgaaggcat gaatgcctta attaattgct cgtgtgtttt ctggtgttat
     5101 tgcgttcttg tttgcagctt cagtttatcc acagattttc agggctaacc aggtgaggtc
     5161 acacaccata aatatcaccc atacagtggc gatggtgagt attgctttta cattcttcat
     5221 aaagtttttg ctttatttac ggcgtttatc gccggtaaag aataattcga tatccttcag
     5281 tgtgtggata attcgcataa tgccgattgc cataagcacg gagataatca gaattaccca
     5341 tgaaaggaat atgctcatgc ggctacccct gtgaagtaag gctcgacaat ttcaccttca
     5401 aacagttctg tcataatcga ctttaattct tccagcgctt cacggtcatc gctgcccagt
     5461 gcgcagttgt atagccccat aattgcgccg cgcagtgagt tacgaaggct ttcatcctta
     5521 gttacgcccc ggtttgcgag ttcaaagtga taacgttgca acgcgttatt gataagtatt
     5581 ttgtaggctt cgatcatggc tgaatccttt atttatcagt gcttgcttcc cggatattga
     5641 aaacgcaggc cgctgattaa tctgtctgcc gttcttaagg cttcaaaaaa tgtgaagtcc
     5701 tgtccgaagt aatcatcccc gcgagtcagg gtgaaattaa atcggccagt aacagggctg
     5761 cgaaaacttt tgtgaataac gatgccagtg tcgccaatcg cccaggtgtg ctttccggtt
     5821 tgttttaccg ggtgaccgtc tggtgtggca tggatttcag tggttacggt cgcactattt
     5881 ttacggaagt gctggcgtgc aacgtggcgt tcggtgttgt tattgatgcg tgcatttcgt
     5941 tgttgcttca ttcttcactc ggtccttatg cgatgattac agtgattcgt ctcttgtcag
     6001 cctaaaaatc acttcagcca tgacttcgct aattacatcg atcccctgat cggtaagggc
     6061 ttctttccag gccttgcttg cacgtgttgg atccatcctg cctgattgct gttgtatgat
     6121 gatgaagcgt aaatcgctgt ataagtcttt tgtttgagtg gagtcagtca tgtcaatacc
     6181 ttcaaatctt gatgttttta atgaaacagt tggtaaaact tttgcgtttc tttatgaaac
     6241 ttttccccgc aaagtaacta ttgatatttg tgccttaacc ggtgtttcag agctgacttt
     6301 tgaagaagac cgatcctcca ggaggcaact agttgaagct cttgagcttc gccgtgactc
     6361 catcgcatgg cttgttgatg ctggttatgt atccgctaaa tcttctcctc cgcattactt
     6421 ttctgatgcg gttctcacgg ccaaaggtct tgaattactt aagcttgatc cagagtcact
     6481 taaacaatca tttggcgaca agcttgttga tgcagccaag agtggttcaa tggatgcgat
     6541 aaaaagcacc gcatcatccg ctcttactac tgggttatcc tttgctttca aaagtcttct
     6601 tggttgattg ttgcgccccc gcataacacc aacgattaga ttatttacgg cgaaatctcc
     6661 tgttgtggat ttcgctgtgt gtttgatatt aagctgcttt tttactgata cgtttattcc
     6721 gtaaaccagc tgctggctta aataaaaaaa gtcggttttc ccattcgtgc cattcttgcg
     6781 gtgcgttttc agtgagtcga tcgcaggctt catcccacgc tttccggttg atgtagagtt
     6841 cggcgtttcc gccaggcttg agagggtccg ccatgtacac agcgggtaat ttccctgctt
     6901 ttgccatgct cacgatggcg tcaacggtct tgccgatata catcgcaaac ccttcttttg
     6961 acaggagatg agaagggttt tgtgaaagcc tgatttcctg gcgttcaggg gctttgcgcc
     7021 cggtcttttg gtgtgcggtt tctggcgcat ctgtcaactg atcgcactgt gtttcggcag
     7081 gcgattcagg cgcgttttga gtgacttctt gcatctggta tcctccgtta ttggtgctat
     7141 ttctatttag caccagttag agccgcctag attgtctcta ggtgtgtttg ttgatgtata
     7201 aactaagaga gttaactcac tatgtcaact aacgtaggtg aaaaaattag aatcatgcgt
     7261 aagtctgaaa tgcttacaca agaaaaaatg gctgaaatta cagggctcac ggtagcggcc
     7321 ctgcgtcaat atgaacaggg gagaaatgag ccaaatcttg aatccacaaa aaagctgctc
     7381 aagtctcccg tcttccgtaa ataccgtgac tggtttttgt tcgatgaggt cgaaccaaag
     7441 gccgggcagg tggtaccggc tctcgcacac attgggcaaa gcgaagcgga atcatcccac
     7501 tccggcaaga agactggtta acggtccatc tggcctttat ttctcaaaat tgtgatcaag
     7561 atgatgtttt cattgggggg cttccttatg tcgattaaga agctcgctga tggcaaatac
     7621 tgtgtggacg tgcggcctgc tggatctgaa ggaagaagat ttcggcggcg atttcctacc
     7681 cgtggtgaag cggcgatgta tgaacgccat gttctgacgc attatcacga taaggactgg
     7741 gtcgaaaaac ctattgaacg taaagcgtta agcgatcttc tggaactgtg gtgggtgtac
     7801 cacggtaaga atcatcgtta tggcgcgatg aatcgggtca gaattgaagc ggttcttcgt
     7861 gatatgcaag agcttggtat taacagaagt gaccacttaa cccgaaaaaa tattatcaga
     7921 tatcgccttg aattaatgaa tcgcggaatt aagcagtcaa cggtaaatcg ttattgtgca
     7981 atgatgagcg gtttctttga aaagctgatt gatgttgaag agtttgtggg tgataacccg
     8041 tttcacgaga tacgcaagct aacaattaat cagccggaaa tggcttatct tgcacatgat
     8101 gatattccca ggctaaccgc acttttatct ggcgataatc ttaaggctgt attgttaagc
     8161 ctggctacag gcgggcgctg gggtgaagtt gccaatatta aaggtgaaca cataattggt
     8221 gggaaggtta ttttcatgga gacaaagaat ggatcgcggc gggttattcc tatagctaaa
     8281 gatcttgaat ctctgattaa agtcaaagcg acgggaagat taatgaaccc cagttatgcg
     8341 atagtcagaa gtgcaataag gactgtgcgg cctgatttac cagtcggtca gtctgtgcat
     8401 gttttacggc acacattcgc aactcatttc atgataaatg gagggaacat tattaccctt
     8461 cagcgtattt tagggcacag cacaattcag cagacaatga cctatgcgca ctttgcacct
     8521 gattatctcc aggacgcggt aaggttcaat ccggttgcgg agttgtccag attttgccca
     8581 taaacgggga ctaaatggca ctattccgaa ctactttgaa ctcgtaacta tttgatatat
     8641 aaagggggat agcgatacgc gctaccccct tatgccacct tcgggtggcg ttgttttttg
     8701 cgagacgact cgcattctgt tttgtaattc cctcaccttt tgcttttctc tccgagccgc
     8761 tttccatatc tattaacgca taaaaaactc tgctggcatt cacaaatgcg caggggtaaa
     8821 acgtttcctg tagcaccgtg agttatactt tgtataactt aaggaggtgc agatgcgtat
     8881 taccataaaa agatggggga acagtgcagg tatggtcatt cccaatatcg taatgaaaga
     8941 acttaactta cagccggggc agagcgtgga agcgcaggtg agcaacaacc aactgattct
     9001 gacacccatc tccaggcgct actcgcttga tgaactgctg gcacagtgtg acatgaacgc
     9061 cgcggaactt agcgagcagg atgtctgggg taaatccacc cctgcgggtg acgaaatatg
     9121 gtaaagaaaa gtgaatttga acggggagac attgtgctgg ttggctttga tccagcaagc
     9181 agccatgaac agcaaggtgc tggtcgacct gcgcttgtgc tctccgttca agcctttaat
     9241 caactgggaa tgacgctggt ggcccccatt acgcagggtg gaaattttgc ccgttatgcc
     9301 ggatttagcg ttcctttaca ttgcgaagaa ggcgatgtgc acggcgtggt gctggttaat
     9361 caggtgcgga tgatggatct acgcgcccgg ctggcaaagc gtattggtct ggctgcggat
     9421 gaggtggtgg aagaggcgtt attacgcttg caggcggtgg tggaataagg tgtgtttatt
     9481 tatcgcgggc ataaaaaaac ccttactaac cgaagcccgg cgttcagggt tattgcgcta
     9541 gaagaactta tttattcttg gcgcgctcga aggaggcaac gatttcagct ttagcggctt
     9601 ctgcgttttc ccaaccttca actttcaccc acttgccttt ttcgaggtct ttgtagtgct
     9661 cgaagaagtg agcgatctgc gctttcagca gctctggcag atcgttaacg tctttaatgt
     9721 gatcgtattc tttgctcagc ttgctgtgcg gaaccgcaac cagtttcgca tcttcaccgg
     9781 cttcgtcggt cattttcaga acgccaaccg gacggcaacg gatcacagaa cccggctgca
     9841 gcgggtacgg agtcgggacc agtacgtcaa ccgggtcacc gtccagagac agggtgtggt
     9901 tgatgtaacc gtagttgcat ggatagaaca tcgcggtgga catgaagcgg tcaacgaaca
     9961 gtgcgccgct ctctttgtcg atttcgtatt tgatcggatc tgcgttagcc gggatctcaa
    10021 taacaacgta gatgtcttcc ggcagatctt tacccgcagg gacgttgagt aagctcatgt
    10081 ctgtttcctt taaaaatatg tggcaaacaa gtgccgagta ttatagccaa ctcgcgccga
    10141 atgtcttcgc ttgttttcgt cttcattttc ctttttcgcc agttttaaga cggtatacag
    10201 agcaggaaaa tccataaccg tagccgcatt tttcatagtg agatgaaagc gattacaaac
    10261 ttgtgattaa cgttttattt acttttttga agtgtgatgt aacgcaatcc gttacataac
    10321 gaattgtcta tagttttttc gcgaacatct tttcaccaat aataattacc ccgacgagga
    10381 caaccctatg tggaaacgct tacttgtagt ctctgcagtc tcggcagcca tgtcgtctat
    10441 ggcgttggcc gctccattaa ccgtaggatt ttcgcaggtc ggatcggaat ccggctggcg
    10501 cgccgcagaa accaatgtgg cgaaaagtga ggccgaaaaa cgcggaatta cgctgaaaat
    10561 tgccgatggt cagcaaaagc aggaaaacca gattaaagcg gtacgttcct tcgttgcaca
    10621 aggggtggat gcgatcttta tcgctccggt ggttgcgacg ggttgggaac cggtattaaa
    10681 agaggcgaaa gatgccgaaa tcccggtctt cttgctcgac cgttccatcg atgtgaaaga
    10741 caaatctctc tatatgacca ccgttactgc cgacaacatc ctcgaaggca agttgattgg
    10801 tgactggctg gtaaaagaag tgaatggcaa accatgcaac gtggtggagc tgcagggcac
    10861 cgttggggcc agcgtcgcca ttgaccgtaa gaaaggcttt gccgaagcca ttaagaatgc
    10921 gccaaatatc aaaatcatcc gctcacagtc aggtgacttc acccgcagta aaggcaaaga
    10981 agtcatggag agctttatca aagcggaaaa caacggcaaa aacatctgca tggtttacgc
    11041 ccataacgac gacatggtga ttggtgcaat tcaggcaatt aaagaagcgg gcctgaaacc
    11101 gggcaaagat atcctcacgg gttccattga cggtgtaccg gacatctaca aagcgatgat
    11161 ggatggcgaa gcgaacgcca gtgttgaact gacgccgaac atggcgggac ccgcttttga
    11221 cgcgctggag aaatacaaaa aagacggcac catgcctgaa aagctgacgt taaccaaatc
    11281 caccctttac ctgcctgata ccgcaaaaga agagttagag aagaagaaaa atatggggta
    11341 ttgagggttg ctatgcctga tgccgattcg taggccggat aaggcgctcg cgccgcatcc
    11401 ggcgatggtg cactgaagcc tgatgcgacg cttaccgcgt cttatcatgc ctactgggag
    11461 cacgctttac accgggggaa accatgacga ccgaccaaca ccaggagatc ctccgcaccg
    11521 aaggattaag taaatttttc cccggcgtca aagcgttaga caacgttgat ttcagcctgc
    11581 gccgtggcga aatcatggcg ctgctcggtg aaaacggggc gggaaaatca acgctaatca
    11641 aagcattaac tggtgtatac catgccgatc gcggcaccat ctggctggaa ggccagccta
    11701 tctcaccgaa aaataccgcc cacgcgcaac aactcggcat tggcaccgtc tatcaggaag
    11761 taaacctgct acccaatatg tcggtcgctg ataatctatt tataggccgc gaacccaaac
    11821 gcttcggcct tctacgccgc aaagagatgg aaaagcgcgc caccgaactg atggcatctt
    11881 acggtttctc cctcgacgtg cgcgaaccgc tcaaccgctt ttcagtcgcg atgcagcaaa
    11941 tcgtcgctat ttgccgggct atcgatctct ctgccaaagt gctgatcctc gatgaaccaa
    12001 ccgccagtct cgacacccag gaagtggagt tactgtttga cctgatgcgt cagttgcgcg
    12061 atcgcggcgt cagcctgatt tttgtcactc actttctcga tcaggtctat caggtcagcg
    12121 atcggatcac cgtcttacgc aacggcagtt tcgtaggctg tcgggaaacg tgcgagctac
    12181 cgcagatcga actggtaaaa atgatgctgg ggcgcgagct ggatacccac gcgctacagc
    12241 gtgccgggcg aacattgttg agcgacaaac ccgttgccgc gttcaaaaat tacggcaaaa
    12301 aaggaacgat cgcaccgttt gatctcgaag tacgccccgg cgagatcgtc ggtctggctg
    12361 gcttgctggg atcaggacgt accgaaaccg ccgaagtgat cttcggtatc aaacctgctg
    12421 acagcggcac tgcgttgatc aaaggcaaac cgcaaaccct gcgatcgcca catcaggcgt
    12481 cggtactggg tatcggcttc tgcccggaag acaggaaaac cgatggcatc atcgccgctg
    12541 cctcggtgcg ggaaaatatc atcctcgcgc tacaggccca gcgcggctgg ctgcgaccga
    12601 tctcccgcaa agaacagcaa gagattgccg aacgctttat ccgccagctt ggcattcgca
    12661 ccccttccac tgaacaaccg attgaatttc tctccggcgg caatcagcaa aaagtgctgc
    12721 tctctcgctg gctgctgact cgtccgcaat ttctgatcct cgacgagcca acgcgtggaa
    12781 ttgacgttgg tgcgcacgcc gagatcatcc gcctgattga aatgctatgc gccgatggtc
    12841 tggcgctgct ggtgatctcc tccgaactgg aagaactggt gggctatgcc gaccgggtga
    12901 tcatcatgcg cgatcgcaaa caggtggcgg agatcccgct ggcagagctt tccgttccgg
    12961 cgatcatgaa cgccattgcg gcgtaaggag aacagtgtga tgcctcaatc tcttccggac
    13021 accactacgc caaaaaggcg ctttcgctgg ccgacgggga tgccgcagct ggtagcacta
    13081 ttgctggtgc tgctggtcga tagcctggta gccccgcatt tctggcaggt ggtgcttcag
    13141 gatgggcgtt tgttcggtag ccccatagac attcttaacc gtgcagctcc cgttgcgtta
    13201 ctggcgattg gcatgacgct ggtgatcgcc accggtggga ttgatctctc cgtaggggcg
    13261 gtgatggcta tcgccggagc cacaacggct gcgatgacgg tcgcgggatt cagcctgccg
    13321 attgttttgt taagcgccct gggcaccggc atcctggcgg gattgtggaa cggcatactg
    13381 gtagcgatcc tcaaaattca gccgtttgtc gccactctga tcctgatggt cgccgggcgc
    13441 ggcgtggcgc aactgatcac cgccgggcag atcgtcacgt ttaactcgcc ggatctctca
    13501 tggttcggca gtggatcgct gttgttcctg ccaacgccgg tcattatcgc ggtgctgacg
    13561 cttatcctgt tctggctgtt gacccgcaaa acggcgctgg ggatgtttat cgaagccgtt
    13621 ggtatcaaca ttcgggcggc aaaaaatgcc ggggtaaaca cgcggatcat cgtcatgctt
    13681 acctacgtgt tgagcgggct gtgtgcggcg attgcgggca ttatcgtggc ggcggatatt
    13741 cgcggtgccg atgccaataa cgccgggtta tggctggagc tggacgccat tctcgcggtg
    13801 gtgattggcg gcggatcgct gatgggcggg cgctttaacc tgctactttc ggtggtgggg
    13861 gcgctgatta ttcaggggat gaacaccgga attttgcttt cgggctttcc gccagagatg
    13921 aaccaggttg taaaagcggt ggtggttctt tgcgtgctga ttgtccagtc gcaacgcttt
    13981 atcagtctga ttaaaggagt acgtagccgt gataaaacgt aatttgccac tgatgatcac
    14041 catcggcgtc tttgtgttgg gttatcttta ctgcctgacc cagtttcccg gttttgcttc
    14101 cacaagagtg atctgcaata tcctgaccga taacgccttt cttgggatca ttgccgttgg
    14161 catgaccttt gtgatcctct ccggtgggat cgatctctcc gtcggttcgg tgatcgcctt
    14221 taccggcgtg tttctggcaa aagtgattgg agatttcggt ctctcaccgc tgctggcgtt
    14281 tccgctggtg ctggtgatgg gctgtgcctt cggcgcattt atggggcttt tgatcgacgc
    14341 cctgaagatc ccggcattta tcattaccct tgcagggatg ttctttttgc gcggtgtcag
    14401 ctatctcatc tcagaagagt cgatcccgat aaaccatcct gtttatgaca cgctctcaag
    14461 ccttgtatgg aaaatccctg gcggcggtcg cttaagtgcg atggggctgc tgatgctggc
    14521 ggtggtggtt atcggcatat tcctcgcgca tcgtacccgt tttggtaatc aggtatacgc
    14581 cattggcggc aatgcgacgt cggcaaacct gatggggatt tctacccgta gcactactat
    14641 tcgcatttat atgctctcca ccggactggc aacgctggcg gggattgtct tctcgattta
    14701 tacccaggtc ggatatgcgc tggcgggcgt aggtgtggaa ctggacgcta tcgcctcagt
    14761 ggtgattggc ggtacgcttt ttggcgtggc gattcaggga ctgattcaga cttacataaa
    14821 ctttgatggc acgctgagct catggtggac gaaaatcgcc atcggtattt tgttgtttat
    14881 ttttatagca ttacagcgtg ggttaacggt gctgtgggag aatcgtcaga gttcgccagt
    14941 gacaagagtc aacattgcgc agcaataaaa acgcctctcc gtgtggagag gcgcaggaga
    15001 ttacgcgtcc gggaactcac ggataaagcg ttcgacatct tcaaccatat ggtcgttgcc
    15061 gacaaagaac gaacggcgct ggtgcagggt ttccgggata atatccagaa tacgctcttt
    15121 gccatcgctc gctttaccgc ccgcttgttc cgccaggaat gccatcgggt tgcactcata
    15181 cagcaaacgc agtttgccgt ccgggtggct ggcggtgctt gggtagagat aaataccgcc
    15241 tttcagtagg ttacggtgga aatccgcaac cagtgagccg atataacgtg aggtatacgg
    15301 gcggttggtg gatttatctt cttcctggca gaatttaatg tacttcttca ccccgttcgg
    15361 aaacttaatg tagtttcctt cgttgatgga gtaggttttg cctttctccg ggaagcgcat
    15421 ccgttcctgg cacaggcaga aaacgccgag cgaaggatcg taagtaaagg cgtgaacacc
    15481 gcatccggtg gtgtaaacca gcatggtaga ggagccgtat accacgtaac ctgccgcaac
    15541 ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc ggcgtgccaa caggcgtaac
    15601 gcggcggtag atggagaaaa tggtaccgac agagacgtta acatcgatgt tggacgagcc
    15661 atccaggggg tccatcagca ccacgtattt tgcgtgttca cagccttcaa agacgacaat
    15721 ctcatcttct tcttcagagg caatgcccgc aacgatatcg cgtgctttca gtgcggcttt
    15781 cagtttttca ttagcgaaca agtcgagttt ctgttgaacc tcgccctgca cgttctcagc
    15841 accgctggca cccaggatat caaccagtcc tgctttgttg atatcacgat ggataatctt
    15901 ggcgcccagt tttattgccg acagcaaagc agtgagctca ccggtagcat gagaaaactc
    15961 gtgctgcttt tcgacaataa attcacctaa cgttttcata aaactttccc tgcaatgttt
    16021 atggagtaaa gcgaccgcaa caatcttaac aaataatctc aatgttgcgc tcaggtgaat
    16081 cgcgccagca aattgcggat tatcctgaaa tgcgtttctc acttgcccga catatgcgta
    16141 aaatgagcgg cagattaaaa aaggatagtg acgtatgcgc attcatattt taggaatttg
    16201 tggcacgttt atgggcggtc tggcgatgct ggcgcgccag ttaggccatg aagtaacggg
    16261 ttcggacgcc aatgtgtatc cgccgatgag caccttactt gagaagcaag gcattgaact
    16321 gattcagggt tacgatgcca gccagctcga tccgcagccg gatctggtga ttattggcaa
    16381 cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg gaaaaaaaca tcccttatat
    16441 gtcaggtcca cagtggctgc acgattttgt gctgcgcgac cgctgggtgc tggccgttgc
    16501 cggtacacac ggcaaaacca ccaccgcggg aatggcgacc tggattctgg aacagtgtgg
    16561 ttacaaaccg ggctttgtaa tcggcggtgt gccggggaac tttgaggttt cggcgcgtct
    16621 gggcgaaagc gacttctttg ttatcgaagc ggatgagtac gactgcgcct tcttcgacaa
    16681 acgctctaaa tttgtccatt actgcccgcg tacgctgatc ctcaacaacc ttgagttcga
    16741 tcacgccgat atctttgacg acctgaaagc gatccagaaa cagttccacc atctggtgcg
    16801 tatcgttccg gggcagggcc gtattatctg gccagaaaac gacatcaacc tgaaacagac
    16861 catggcgatg ggctgctgga gcgagcagga gctggtgggt gagcagggtc actggcaggc
    16921 gaaaaagctg accaccgatg cttccgaatg ggaagttttg ctggatggcg aaaaagtggg
    16981 cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac aatggcctga tggcgattgc
    17041 agcggctcgc catgttggtg tagcgccggc agatgccgct aacgcgctgg gttcgtttat
    17101 taatgctcgt cgccgtctgg agttgcgtgg tgaagcgaat ggcgtcacgg tatatgacga
    17161 ttttgcccat cacccgacgg cgattctggc aacgctggcg gcgctgcgtg gcaaagttgg
    17221 tggtacggcg cgcattattg ctgtgctgga accgcgctcg aataccatga aaatggggat
    17281 ctgcaaagac gatttggcac cttctttagg tcgtgccgat gaagtcttcc tgctgcaacc
    17341 ggcgcatatt ccgtggcagg tggcagaagt ggcagaagcc tgcgttcagc ctgcacactg
    17401 gagtggcgat gtggatacgc tggcagatat ggtggtgaaa accgctcagc ctggcgacca
    17461 tattctggtg atgagcaacg gcggttttgg tgggatccat cagaaactgc tggatggtct
    17521 ggcgaagaaa gcggaagccg cgcagtaatt cggccttagc ctgatatagt gtgcgtaggc
    17581 cggataaggc gttcacgccg catccggcat ttgagcatag tgcctgatgc gacgcttgat
    17641 gcgtcttatc aggcctacag gtgcaccgca tccggcaaac catactcaca tcaacaacga
    17701 aaattaccct tcattctccg ccagttcgcg cagatactgg aaaatctggc gtgcggattt
    17761 cggcggctta ttcccttctt tctctttctt cgcgttgcgg atcagagtac gcagttgctg
    17821 acgatcggca tcgggccaca gatttaatac ctcagcgatg gcgtcatcac cttgatcgat
    17881 cagacgatcg cgcaggtttt ccagtttatg gaacagcacc acctgctggt tgtgacggtt
    17941 tttcagctta tccagcgcct gacgaatagg ctctacgtcg cgctggcgca gcatcttacc
    18001 gatgagctgc aactggcggc ggcgaccttc catcttaata cgctgggcga gctcaatagc
    18061 cgcgcgcaga tccgcatcta acgggatctt atccagcgcg tttttcccca gatcaacaat
    18121 ttccgcgcca aggcgtttta gctcctcggc atcacgttta atttcacttt tactgaccca
    18181 gataatttca tcgtcttcgt cttcgatgtc atcaccggga acgtcgtcga gccagtcttc
    18241 gggctgctta gtcatctcag gctccttaaa aaaagaggct aatgttacca gttaagatgc
    18301 gcactgaaaa acggttctct gttagacttc agagaaactc tctacattat ggcacttgca
    18361 atgaaagtaa tctctcaagt tgaagcgcag cgcaagattc tggaagaagc agtttccact
    18421 gcgctggagt tggcctcagg caaatcggac ggtgcggaag ttgccgtcag caagaccacc
    18481 ggcattagcg taagcacgcg ttatggtgaa gtggagaatg ttgaattcaa tagcgatggc
    18541 gcgctgggga tcactgttta tcaccagaac cgcaaaggta gcgcatcatc caccgattta
    18601 agcccgcagg ccattgcccg tactgtacag gcggcgctgg atattgcccg ttatacctcg
    18661 ccagatccct gtgccggcgt ggcagacaaa gagctgctgg cctttgacgc accagatctc
    18721 gacttgttcc accctgcgga agtttccccg gatgaagcca ttgaactggc ggcccgcgca
    18781 gaacaggcgg cattgcaggc ggacaaacgc atcaccaata ccgaaggtgg cagctttaac
    18841 agccactacg gtgtcaaagt ttttggcaac agccacggca tgttgcaggg ttactgctca
    18901 acgcgtcatt cgctctccag ctgtgtaatt gccgaagaaa atggcgatat ggagcgtgat
    18961 tacgcctaca ccattggtcg tgcgatgagc gatctgcaaa cgccagagtg ggttggggcc
    19021 gactgtgctc gccgtacttt atcgcgcctg tcaccgcgta aactctccac catgaaagcg
    19081 ccggtcattt ttgccaatga agtggcaacc gggctttttg gccatctggt gggggcgata
    19141 gcgggtggat cggtttaccg taaatctacc ttcctgctgg attcgctggg taaacaaatt
    19201 ctgccggact ggctgaccat tgaagagcat ccgcatctgc tgaaagggct ggcgtcgacg
    19261 ccattcgaca gcgaaggtgt gcgcaccgag cgtcgcgata ttattaaaga tggcatcctg
    19321 actcagtggc tgctgaccag ctattcggcg cgaaaactgg ggctgaaaag caccggacat
    19381 gcgggcggta ttcacaactg gcggattgcc ggacaaggtc taagcttcga gcagatgctc
    19441 aaagagatgg gcaccgggct ggtggtgacg gaattgatgg gccagggcgt gagtgcaatt
    19501 accggtgatt attcccgtgg tgcggcgggc ttctgggtag aaaacggcga aattcagtat
    19561 ccggttagcg aaatcaccat cgcaggtaat ttaaaagata tgtggcgcaa tattgtcacc
    19621 gtcggtaacg atattgaaac acgcagtaat atacagtgtg gttctgtgct gttgccggag
    19681 atgaaaatcg ccggacagta aaaaaactgg cgcgacctgc cgcgctataa actaaaaaat
    19741 tacacaaaat cattcgcact gcatcgagac ggaaagtgaa tgagggccgg ggagcgtact
    19801 cgcagtacgt gatccggtcg aatgagcgta gtcgtcgatg aggcagtgtg aaggatgaag
    19861 tgtaaataaa aaaggaagtg agcaatgcga aaaagcctgt tagctattct tgcagtctcc
    19921 tcgttggtat tcagttcggc gtcgtttgcc gctgatcttg aagacaatat ggaaaccctc
    19981 aacgacaatt taaaagtggt cgaaaaagcc gataacgcgg cgcaagtcaa agacgcgtta
    20041 acgaagatgc gcgccgcagc gctggatgcg caaaaagcaa cgccgccgaa gctcgaagat
    20101 aaatcaccgg acagcccgga aatgaaagat ttccgccacg gtttcgacat tctggtcggt
    20161 cagattgacg acgcgctgaa gctggcaaat gaaggtaaag taaaagaagc gcaggctgcc
    20221 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attccttatt
    20281 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
    20341 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
    20401 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
    20461 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttgtaccagt
    20521 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaataccc
    20581 tggcgtttga tacgagtgtc attcagatcg ttaatgatct ggtcttccat tgctttagta
    20641 aatggctgac cggaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
    20701 acacacccgg agacaaacag ggtgcagcga gtgccgggac cgttgacgat gtcgacagga
    20761 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgtatca
    20821 ggcaatcaac gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
    20881 gtaggccgga taaggcgttt acgccgcatc cggcaatcaa cgcgggatgc gacgctttca
    20941 catcacatcc ggcatttgct tagtgcagat caacctatct gcccatttcc caaatgttta
    21001 acgcggcgct taacttcttc ctgcttacca gcgttaaacg gacgtgcatc cgggctacct
    21061 aaatatccgc acacgcggcg agttaccgac acacgggagg cgtcatggtt accacatttc
    21121 gggcaagtga agcctttgct ggtgcactcg aactcaccgg taaagccaca ctcgtagcac
    21181 tcatcaatcg gtgtattggt gccgtaatac ggtacatgct gatagctgta atcccagaca
    21241 tcttccagcg ccttcaggtt gtgctgaatg tttggatact cgccgtagca aatgaaacca
    21301 ccgttcgcca gcggcgggta aggcgcttca aagtcgatct tgtcgtacgg gttcaccttc
    21361 ttctccacat cgaggtggaa actgttggtg tagtaacctt tatcggtcac gcccggcacc
    21421 acgccaaact cagcagtatc gagacggcag aagcggtcgc acaggttttc actcggcgtg
    21481 ctgtagagac tgaaaccata acccgtttct tctttccact gatccactgc ctgacgcaga
    21541 cgttcaacaa tggcgatacc tttcgcgcga agctgctcgt tgtcgtaaac atgctcgccg
    21601 ccgaacagcg cgttaatggt ttcgtggatg ccgatgtaac ccagcgaaat agacgcacga
    21661 ccgtttttga agatttcaga aacatcatcg tcagcattca gacgcacgcc acaagcacct
    21721 tccatataga ggatcggggc cacgcgcgct ttcacgcctt cgagacgagc gatacgggtc
    21781 atcagcgcct tacgtgccag caccagacgt tcatccagca gcttccagaa ggtggcttca
    21841 tcgccttttg cttccagagc aatacgcggc aggttcaggc tgatcacgcc gaggttgtta
    21901 cgaccatcgt ggatctgctc gccgttttca ttttcccaca cgccgaggaa gctgcggcag
    21961 cccatcgggg ttttaaacga accggtgact ttcactacct gatcgtagtt caggatatcc
    22021 ggatacatgc gcttgcttgc gcactccagc gccagctgtt tgatgtcgta gttcggatcg
    22081 ccttttttat ggttcaggcc atcgcgaatc gcaaacacca gtttcgggaa caccgcagtt
    22141 ttacggtttt taccgaggcc tgcgatacgg ttacgcagga tagattcctg aatcaggcgc
    22201 gactcccagc tggttcccag accaaaacca aaggttacaa acggcgtctg accgttggcg
    22261 gtgtgcaggg tgtttacttc gtactccagc gactggaagg catcgtagca ctctttaatg
    22321 gtacgagagt tagcgtagcc ttcggcgtcc gggatgttcc actcttctgc ggtcttgcga
    22381 tgtttgttgt agctggcggt gacaaacggt gccagcactt catcgatacg gttaatggtg
    22441 gtgccgccat aaatatggct ggcgacctga gcaataatct gcgcagttac agcggttgcc
    22501 gtagagatcg acttcggcgg ttcaatctcg gcgttcccca tcttaaagcc ctgggtcagc
    22561 atgcctttca ggtcgatcaa catgcagttg aacatcggga agaacggcga gtaatcgaga
    22621 tcgtgatagt gaatatcgcc acgctcatgt gcctgcacca cgtcacgcgg cagcaggtgc
    22681 tgacgtgcat agtgtttagc cacgatcccg gccagcaggt cgcgctgggt tggaatcacc
    22741 ttgctgtctt tgttggcgtt ttcgttgagt aacgaggcgt tggtctgctc gaccagacca
    22801 cggatctcct gattcaggcg accgcgtttt tcacgttcaa tgtcgcgatc gtgacggtac
    22861 tcgatgtaag cacgagccag ttgtttgtat ggacctgaca tcagctggtt ttcaacagcg
    22921 gtctggatct cattgatatc cacctggttg cggccctgca tctgctcgct gacaactgca
    22981 gcaacagtgg cgcaataatc ggcatcatcg acttccgctg ctttagctgc acgcagaatc
    23041 gcttctttga tgcgctctga tttaaacggc actttgcagc cgtctcgttt catcacatgc
    23101 ggtgtcatga tcgctccata tttttaagaa caggttatcc acagaaattg ggaaaggcgt
    23161 tttccggttt tttcgtttcg ttttccggtg ctttccgcaa tcgccatccg cttatccaca
    23221 acgccgcacc tttttcgtgg ggagctgtag tagcaattat agtcgattaa tacaacatat
    23281 tgggttggga cgcattttaa agtctatata tagtgctttg catcaaggat gtttgaactt
    23341 tttttgatgt agctcaaagt aaaaagcaga gcgtacggat gacgggcgct acagcgttat
    23401 gtaaattttt taatgaattt gctggttaaa aaatcaacaa aaacaacata ctgacagaca
    23461 aaaccccgga atgaccggca ttaccggggc ttagggaaga tttacttctg taaccaccag
    23521 acagcctcaa aaggccgtaa agtcatggca cagggttgtg gtgaggcttc ttcgtagtta
    23581 tgcatcacaa gctgccagtt gccgcgcatt tgctctggct gccagggttg gatctcacgg
    23641 ctaaggttgg caatgaccag caaggtttgc cccttccatt cacggcgata gcaccacaat
    23701 acagggctgt ttggcagcag atcctggtaa ttgccccatg tcagaacggc ttcctgctta
    23761 cgcagtgcga ttaacttttg gtaggtgtaa aacaccgagg actcatcggc cagcgcggct
    23821 tctacgttga tttgttgata gttatcgccc aggccaatcc acggttcgcc agccgtaaac
    23881 ccggcattat cgccgttgct ccattgcatg ggcgtgcggc tgttgtcacg ggatttactg
    23941 gcgaggattg ccaataactc gtcggcatca cgcccatcgt tgcgcagctc ggcaaacata
    24001 ttgaggctct ctacgtcgcg atagtcagta atgcgcgtga aatgcgggtt ggtcatgcca
    24061 atctcttcgc cctggtagat atacggcgtt ccctgcatgc catgcagcac catcgccagc
    24121 atttttgccg caggcacgcg gtattcacct tcatcaccaa agcgagaaac aatgcgcggc
    24181 tgatcgtggt tacaccagaa caaggcattc catgctacgt tgtgcattcc ttgttgccag
    24241 tggcggaaca atgttttcaa cgccacaaag tcaggtttag ccagcgtcca tttttcacca
    24301 ccgggataat cgaccttcag gtgatgaaaa ttaaaggtca tcgacaattc actgccagtc
    24361 agtgccgcgt atcgctggca atgctcaagg ctggtggagg acatttcacc tacggtcatt
    24421 aacccgcgtg gcgtaaacac atcgcggttc atctcgtgca aaaactcgtg tgctcgtggc
    24481 ccgtcggtgt agaagcgacg cccgtcgcca tccaggtcat cagggaaacg cgggtctttg
    24541 gagatcagat tcaccacatc caggcgcaac ccgtcgaccc cacgatcggc ccagaactca
    24601 cagacttttt tcagctctgc gcgtaccgct ggattctccc agttgagatc cgcctgttct
    24661 ggtgcaaaga gatgcaaata gtactgttcg ctttccgcat gccagcgcca cgcactaccg
    24721 ccaaattttg aacgccagtt gttcggtggc gtttctggtt ctccatcgcg ccagatataa
    24781 aactggcggt aagggctttc tttgttcagc gcctcgcgaa accaggcatg ttgggtagag
    24841 gtatggttaa acaccatatc gagaatgata cgaatcccgc gcgattttgc ctgcgtcacc
    24901 agttcgtcaa aatcgtccag cgtgccgtag gtgggatcaa tcgccgtata gttcgctacg
    24961 tcgtaaccgt tatcgacctg gggagagaca taaaaggggg ttagccagat ggcatcaacg
    25021 cccagtttat gcagatagtc caggcgttgg ataacgccac gtaaatcgcc ggtgccgcta
    25081 cccgtggtgt cctgaaaact ctttggataa atctggtaga taacgccgtt ttgccaccag
    25141 tggggaagat tagtcattac gttattcctg caaatgcgag ggggcgcaat tgcgccccga
    25201 agaaaattaa acaatgtcca gcgtgcccag gcggtatttc cgctgataga taaacgaggt
    25261 gagtacaatc gggatgatga tggcgatagc cattgccagc gcaaacacct gccagtagct
    25321 cggttgaatc gagagaattc ccggcaggcc gcctacgccg atgccattcg ccataacgcc
    25381 gttcaggccg catagcaatc ctgccagacc agaaccaatc atcgcgcaca gcatcgggaa
    25441 gcgatatttc aggttgatgc cgtacattgc aggctcagtg accccaagcc aggcggagat
    25501 cgccgcaggc acggagatct cgcgttcatt gtgtttgcgg ctggaaatga taatgcctat
    25561 cacggcggag ccctgagcga tattcgacag cgcaatcagc ggccacactg gcgtaccacc
    25621 catgctttga atcatctgca aatcaatagc aagcgtggtc tggtgtaccc cggtgatcac
    25681 cagcggggcg tacaggaagc caaacaatgc cgcgccaatc ggagcaaagc tgccggtcat
    25741 caggtgacgt accgcaaagg caacgccatc gccaatcatg cgaccaaacg gaccaatcag
    25801 cgcatgggcg aggaacaccg cgaggatcag cgaacagacg ggcaccacca ccagatagag
    25861 gtaatctggc acgatgcgtt taaggcgagt ttcaataacg cccagcgcca gcccggctaa
    25921 tagtgccggg atcacctgcg cctgatagcc cactttggcg atgctgaaca tgccaaagtc
    25981 ccacacttcc ggcagctgct gcccgagcag ataagcgttc atcagctggg gggaaaccag
    26041 tgtcacgcca agcacgatac caaggatcgg cgtgccgccc atttttttca ccgctgacca
    26101 gcaaatcccg accggcaggt agaagaagat cgcttcaccg atcaaccaca gaaaatcgta
    26161 gatcgtttgc agggaagggt acatttgcgc cagcgtttga ccgttgctca tgggcaaatc
    26221 gccgatcaca ttgcgaaaac cgaggatcaa accgccgcta atcaacgcgg gcagcaacgg
    26281 gaagaagatc tctgcgaaat gagagatcaa ctgctcatgc catttcatat tctgccgggc
    26341 ggcttttttt acctgctctt tatcaacctg cgcctgtccg gttgacgcta tcagtgcttg
    26401 atagtaatca cccacgttgg tgccaatcac cacctgaaat tgcccggcat tggtgaaaca
    26461 gcctttcacc atggggagtt gctcaatttc tttcggtctg gcattggccg gttggttgag
    26521 gacaaagcgt aggcgagtaa tacagtggct caccgtcgca atattgccgc gcccgccgac
    26581 cagttcaatc aaccgatcga tatccgtttg gtttattttg ctcatcataa agccccatgg
    26641 cagatgacat ttttggttgg ctgcagaata tattgcgcgg aaaaatttaa aaacgggaac
    26701 gttcccgaaa cgcagcgaag atcacaattt atcgttcagg aaacgatcag gacagggtgg
    26761 cggggatgat gatttgttgc ggttcgctgc gtccggttac ctgtgcgatc aactggcagg
    26821 ccgcctggcg tccagcttcg gcgtaaccgg gatctacggt gacgatctcc ggatggagga
    26881 atttcattaa cggcgtatta ccgacgctcg ccagttgcaa ggtgtcgatg cgttgctctt
    26941 gcaggtattt acttgcgcca agtgccagcg tgtcggttgc gcacagtaag gcggtagttt
    27001 caggcgtaat cacttttgct acgttctcat agccttgctt catagcaagg cctggcagag
    27061 cggcaacggg atgcagttta tgcgctttgc agaacgccag gtaggcttcg tgacgtcgct
    27121 taccggtcgt cacgtcactg tgcggcacgc cgagataact gatattacga tgcccctggt
    27181 catacagccg ttgcatcagg atcttgattg ccccttcgtc gtcataacag accgaagcaa
    27241 agccttttgc gtcacgcgcc agcagaacca gcgatgactg ccagtgggct aacatttctt
    27301 ctgttatgcc agtaaaaccg aacagcacta cgccgtcgat attacgccgt ttcagcaccc
    27361 ccaaatgttc ggcaactaat tgcggggaaa actgactttc catcatgatt gggtcgtaac
    27421 cttgttcata gaacgctggc agcatggttt gaacggcgag attttctgac aacgaatcca
    27481 gacgggtaac aatgatggcg accactttat cgctttgccc acgcatagcg cgcgcagagc
    27541 gggaagggga aaatccatgc tgattcatca ctgcttcaac acgctcgcgg gtgcgctggc
    27601 tcacgccgct ttcgttattc agcacccggg aaactgtaga tttccccacg ccgcttaagc
    27661 gcgcgatgtc tttgatggtc agccgatttt gcatcctgtt gtcctgtaac gtgttgttta
    27721 attatttgag cctaacttta cccgtgcatt cagcaatggg taaagtctgg tttatcgttg
    27781 gtttagttgc cagcaggtat tatatcgcca tagatgctac gaatattatt ggattctcct
    27841 tattatttgc ggcgcttttt tcacttaccg gaggttatat ggaacctgat cccacgcctc
    27901 tccctcgacg gagattaaaa cttttccggt aagcccgtct tttcacggcg ttaccggatg
    27961 cgtaaggccg tgacgtttta acgtccctgc tcagctttat taccttcagg taaggcttcg
    28021 ccacgcctga agacatttct gtactgtttc agacagtgcg gagggactcc ttatgtttaa
    28081 agaaattttt acccggctca ttcgccattt accttcccgt ctggttcatc gtgatccatt
    28141 gcctggcgcg cagcagacag tgaatacggt ggtcccgccg tccttaagtg cgcattgcct
    28201 gaaaatggcg gtgatgcccg aagaagaatt gtggaaaacg ttcgacaccc atccggaagg
    28261 gttaaatcag gcggaagtgg aatctgcccg cgaacaacat ggtgaaaata aattacccgc
    28321 acaacaaccg tcgccgtggt gggtacattt atgggtctgc tatcgcaacc cctttaatat
    28381 tttactcacc attctcggcg ctatttctta cgccacggaa gatttatttg ctgcgggcgt
    28441 tatcgcgcta atggtcgcta tttctacgtt gctgaacttt attcaggaag cacgttccac
    28501 taaagcggca gatgccctga aagcgatggt cagcaatact gcgacggtgc tgcgcgtaat
    28561 taacgacaaa ggcgaaaatg gctggctgga gatcccgatc gaccagctgg tgcccggcga
    28621 tattataaaa ctggcggcgg gagatatgat cccggcagat ttacgtatct tgcaggcgcg
    28681 ggatctgttc gtcgctcagg cgtcgttaac cggtgagtct ctgcccgtag aaaaagccgc
    28741 taccactcgc cagccggagc acagcaatcc gctggagtgc gacacgctgt gttttatggg
    28801 caccaccgtg gtgagcggca cggcacaagc aatggtgatt gctacaggtg ccaatacctg
    28861 gtttggtcaa ctggcggggc gtgttagtga gcaggaaagc gagccgaatg cctttcagca
    28921 agggatcagc cgcgtcagta tgctgctgat tcgctttatg ctggtgatgg caccggtggt
    28981 gctgttaatc aatggctaca ccaaaggcga ctggtgggaa gcggcgctgt ttgcgctttc
    29041 ggtcgcggta ggcctaacgc cggaaatgtt gccgatgatt gttacctcga cgctggcgcg
    29101 cggcgcagta aagctgtcga aacagaaagt gatcgtcaaa catctggatg ctattcagaa
    29161 ctttggcgca atggatattc tgtgcactga taaaaccggc accctgacgc aggataaaat
    29221 tgtgctggag aatcataccg atatctccgg taaaaccagc gaacgcgtgc tgcatagcgc
    29281 gtggttgaac agtcattatc agaccggact taaaaacctg ctcgatacag cggtgctcga
    29341 aggtacggat gaagagtcag cgcgctcgct ggccagtcgt tggcaaaaaa tcgatgagat
    29401 tccgtttgat ttcgagcgtc gccggatgtc ggtggtagtg gcagaaaata ccgagcacca
    29461 tcagctggtt tgcaaaggcg cattgcagga aatcctcaat gtgtgttcgc aggtgcgtca
    29521 caatggcgag attgtgccgc tcgatgacat catgctgcgt aagattaagc gggttactga
    29581 tacgctgaat cgtcaggggc tgcgcgtggt tgcggtggcg acgaaatacc tgccagcgcg
    29641 tgaaggagat taccagcggg cggatgaatc cgacctgatc cttgaaggat atattgcttt
    29701 tcttgatccg ccaaaagaga caaccgctcc ggcactgaag gcattaaaag cgagtggaat
    29761 taccgtaaaa atccttactg gcgacagtga gttagttgca gcgaaagtgt gccatgaagt
    29821 ggggctggac gcgggagagg tggtcattgg tagtgatatt gaaacgctat ctgacgacga
    29881 actggcaaat cttgcacagc gcaccacgct gtttgcccgc ctgacgccga tgcataaaga
    29941 acgcattgtg accttactga agcgcgaagg gcatgtggtt ggctttatgg gcgatggtat
    30001 taatgatgcg cccgccttac gcgcggcgga tatcggcatt tctgtggacg gcgcggtaga
    30061 tattgcccgt gaagcggctg atatcatcct gctggaaaaa agcctgatgg tgctggaaga
    30121 gggggttatt gagggacgtc gcactttcgc caacatgctg aaatacatca aaatgacggc
    30181 gagttctaac ttcggtaatg tgttcagcgt gctggtggcg agtgctttct tgcccttcct
    30241 gccgatgttg ccgttacact tgcttattca gaacctgctg tacgatgtgt cacaggtggc
    30301 gatcccgttt gataatgtcg acgacgagca aattcaaaag ccgcagcgtt ggaatccggc
    30361 ggatctgggg cgctttatga tcttcttcgg accgatcagc tcgatcttcg atattctgac
    30421 gttttgcctg atgtggtggg tattccatgc caacacgccg gaaacgcaaa cgctgttcca
    30481 gtcgggatgg tttgtggtgg gcttactgtc gcaaacgctg attgtgcata tgatccgcac
    30541 ccgccgtgtg ccgtttattc agagctgtgc atcgtggccg ttaatgatca tgaccgtgat
    30601 cgtgatgatt gtcggtatcg cattgccgtt ttcaccgctg gccagttatc tgcagctgca
    30661 ggcgctgccg ttaagctatt tcccgtggct ggttgcgatt ctggcagggt atatgacatt
    30721 aacccagttg gtgaaagggt tctatagccg tcgttacggc tggcaataaa gaatcaactg
    30781 ggcatggtga cccggattca gattgcagac aaagtgagtt ttgttcatgc cggatgcggc
    30841 gtaaatgcct tatccggcca acaaattcaa taaattgcag gaatcacgta ggcctgataa
    30901 gcgtagcgca tcaggcgatt ttgcgtttgt catcagactt aatccgggca tgatagcccg
    30961 gatttccatc aagattagcg acgaacagcg atcgcttcga tctcaatctt cacgtctttc
    31021 ggcagacggg ctacttcaac gcaagaacgt gccgggaagg tggcgttgtg ttcggtgaag
    31081 aaggcttcgt aagtggcgtt tacggttgcg aagtcgttca gatcttttac aaacacggta
    31141 gttttaacga tgtcgcccac tttcaggcca gcggcttcga cgatcgcttt tacgttatcc
    31201 agcgactgac gtgcctgtgc agcgacgtct gccggtactt cgcccgtttt cggatttacc
    31261 gggatctgac cggaggtgat gatcatattg cccagatcaa cgccctgtac gtaaggaccg
    31321 atagctgccg gtgcattttc cgtcgcgata gttttgctca tgatttctcc tttattacag
    31381 cggtaaaaag tctggctcat tatagggagc cagactttta ttaccaaccg caattaattg
    31441 gccagcacca cattatggga aaactctttt tcacagtatt tgcatttgag cgcgatatca
    31501 ttggcgcgtt ttcgcacggc aaagctggaa gaaaccggtt cggcatggct gatacagttg
    31561 ctgttcgggc agaccagcac attgtcgatg cgctccggca gacttgggcg cgatttaccc
    31621 accacttcat agttgtcgat acggttaacc gtggcttgcg gcgcatacaa tgccagttga
    31681 tctacttgat cttcactcaa aaaggtattt tcgattttga ttagatcttt gcggcccatc
    31741 tcgccagaag gcaggttcag accgatggta atgcgctgat ccgtttcggt cagcttgaac
    31801 agactcaaca gcttaaaacc gatctgggcg gggatatggt caattaccgt gccgcgttta
    31861 atagcttcaa cctgcaattt attatcgtgt gtcatctcta tttcccctta cagtaccaga
    31921 tcgcgattca gaaccagtgc cagtaacgcc tggcgagcga aaatcccatt gcctgcctgc
    31981 tggaagtacc aggcgtgtgg cgttttatca acatccgtcg caatctcatc aacacgcggc
    32041 agcggatgca gcactttcat attggctttg gcgttgtgga gatcgctggc gcgaagaaca
    32101 aactgcgctt tcacgttggc gtactcggac gggtccagac gctctttttg cacgcgggtc
    32161 atgtacagga tgtcaacttc cgccatcact tcttcaatag agctgtgcag actccatgcg
    32221 atcccttttt catcgagcat atccagaatg tattgcggca ttgccagcgc gtccggcgcg
    32281 atgaagtaaa aacggttgcc gtcgaacttc gctaacgcct gagtcaggga gtgaacggtg
    32341 cggccatatt tcaggtcacc aaccattgcg acgtggagat tgtccagacg cccctgggtt
    32401 tcctgaatag tgaataagtc cagcaaggtt tgcgtcggat gttggttgga gccatcaccg
    32461 gcattcagta ccggtacatt gccggaaaac tcggtggcca ggcgcgccgc accttcctgc
    32521 ggatgacgca tcactatcgc atcgacgtaa gtgctgataa ccgaaatggt atcggccagc
    32581 gtttcgccct ttttacccag tgatgtattg gcgctgtcgg agaagcccac cacgctggca
    32641 cccaggcggt gcatggaagt ttcgaaagag aggcgggtac gggtagaggc ttcgaagaag
    32701 cagctggcaa tgactttgtg tttcaacagc tctggttgcg ggtttgcttt cagttttgcc
    32761 gctgttgcca gcaccagatt aaggtcatcg cgactaaggt cgtttatgga aatgatatgt
    32821 ttctgatata gcggattagc catcttttat ctcctgacgc ctgggcaaaa aaaagcccct
    32881 cgattgaggg gctgggaatg ggtgatcaac gggaagaaaa acggcaggcc agcgtctttt
    32941 ttcagacgcg gtaagacaaa atgtcgaaca cactgaacca tacatcctcc cggcaaattg
    33001 tccggcatta tactaatcgt cagaagcggt gcaagcattt gatgcaatat tttgtcagcg
    33061 caaacggttt atttgaattt aaagtcaaaa tatatgcatt tttatttact gtgattctgg
    33121 aggggaactg tcgttcggta tcaataattg cagacatttc ctgctttttt ctttttcact
    33181 ttcacgcaat cagatatgca ttttattcca ttcatccgac ttatagggcg gagtgtgaaa
    33241 acgaacggct aacactattg cttacagctc agggatgcgc gctatcactt taatttcaaa
    33301 atcaaagcct gccagccatg taacacccac cgccgtccag tttggataag gtggggcgct
    33361 aaatatttca tttttcaccg tcatgatgtc ttcgaattgt ttttctggat cggtatgaaa
    33421 gctcgtaata tcaatgatat tgtcaaaagt gcatcccgca gcagccaggg tcgcatgcaa
    33481 attatcaaat gccagtctga cttgttgctg aaaatcgggt tctggtgatc cgtcctctcg
    33541 acttcctact tgcccggaaa caaacagcaa atcgccggaa cgaatagccg cagaataacg
    33601 atgttctgca tacagtgaat gtcggccagc agggaaaaca gcggttcttt ctaccatttg
    33661 gttatcctca agatttacga catgaacaga agatttatat ttaccgggag ccgcttttag
    33721 cggacgacgt gagtaaacaa aacccagaca tcatggataa tggctgggct taattgagcg
    33781 tagtcggtta tgcgccaaac gcgccatcaa tggtatgcat cgcgccggta acaaaactgg
    33841 cttctggccc tgctaaccat gcgaccatac cagcgacctc ttccggttgc ccatgtcttt
    33901 tgatggccat aaaaccatgc aacatatcgc gcattggccc gttggcggga ttagcgtcgg
    33961 tatcaattgg ccctggctgg acgacgttga tggtgatccc acgcggtcca aaatcacggg
    34021 ccagcccgcg cgccatgcct tgtagggcag atttactggc ggcataagca gccatgcctg
    34081 caacaggcat acgatcgcca ttcacggagc cgatgattaa gatgcgcccg ccttcgggca
    34141 tctgccgggc ggcttcaaca gaggcatggt aaggagcatg aatattgatt ttgaaaaggt
    34201 gatcaatatc gtcggcattt aattccaggg catcgccaaa gacgccaata cctgcattta
    34261 ccaccaggat atccaatgcg ccgctcttac gaacgacatc aataacagcg tctctgtcag
    34321 cactatctgt gaatactgct gtcgctccag tctcttgtgc caggcgttca gcggcatctt
    34381 tcgaccccgc ataggtgaat cgtacattgg ccccatcggt gacgaaacga cgtacgatag
    34441 cggcaccgat tccacggcta ccaccgagga tgagaactgt cttacctgta aaagcgccca
    34501 taaggactcc ttgatttatt atgtaacatg cattacaaaa ctgttttaac tttctgtcaa
    34561 caagttttgt aatggtcact aaaaaacaat ctcgcgttcc aggtcgtccc agacgtttcg
    34621 ctcctgagca ggcagtctct gcggcaaaag tgctttttca ccaaaaaggt ttcgatgctg
    34681 tcagtgttgc tgaagttact gattatcttg gtattaaccc cccgagcctc tacgcggctt
    34741 ttggcagtaa ggctgggtta tttagccgtg tactcaatga atatgtcggt acggaagcta
    34801 ttccgcttgt cgatattctt cgtgatgatc gtccggtagg ggagtgcctg gctgaggtat
    34861 taaaagaagc ggcgcgcaga tatagccaaa acggcggctg cgctggctgt atggttcttg
    34921 aaggcattca tagtcatgat ccacaagcgc gtgatattgc cgttcaatat tatcacgccg
    34981 cagaaacggc catttatgac tatatcgcca ggcggcatcc acaaagcgca caatgtgtga
    35041 ctgattttat gagtaccgtg atgtcagggc tttctgcgaa ggcacgagag gggcactccc
    35101 tcgaacaact ctgtgcaaca gctgcactgg cggggggagc gataaaaact attctcaagg
    35161 agtgatgcgg gccttgaccc gaaaggcggg aacgcgcctg ccgataagtt gtgataagac
    35221 aataattcac gcgttaaggc tagcggaatt gatcatcttt tcgtataacg ctagaaatga
    35281 aacgttgttt taattaagga gtggaaaaga tgatcatcgg aaatattcat aatcttcagc
    35341 cgtggctacc ccaggagtta cgccaggcga ttgagcatat caaagcacac gttacggtag
    35401 aaacgccaaa gggcaagcac gatatcgaag gcaatcgtct gttttatctt atctcggaag
    35461 atatgaccga gccgtacgaa gctcgccgtg cggagtacca tgcccgctat ctcgacattc
    35521 agattgtgtt aaaaggtcag gaaggcatga ccttcagcac acaaccggca ggtacgccgg
    35581 ataccgactg gttagctgat aaagacatcg catttttgcc ggaaggcgtt gatgagaaaa
    35641 cagttatcct taatgaaggt gattttgttg tgttttatcc gggggaagtg cataaaccgc
    35701 tgtgcgcagt gggcgcgcca gcccaggttc gcaaagcagt agtgaagatg ctgatggcgt
    35761 gatgactttt cgccgtaaat aactccaggt ttacggcgag tttgtgaaaa gagcgttttt
    35821 tgatattttt ttgtgagtaa aatttgtaat gcttagacgt tcttattaac tcaaggagtt
    35881 cgtcatgagc aaaatatcag atttgaatta ttctcaacac attacatcag ccgataattt
    35941 taaaccaaaa aatgaagtct taaatacctg gtgtgtggga atgaatgatt ttgcccgtat
    36001 tgccgggggg caggatagca gaaggaatat tctttctcct ggagcatttt tggagttttt
    36061 ggcaaaaata tttaccctgg gttatgtgga ttttagcaaa cgctccaacg aagcgggtag
    36121 aaatatgatg gctcatattg agtcctcatc ttatatcaaa aataatgatg gcagtgaaat
    36181 aatgaagttt gttatgaata atcctgaagg tgagcgagcg gattcatcta aggtgattat
    36241 agaaatttca ctttccacta ctactatggg gactcgtcaa ggacatacag ccattatatt
    36301 tccacaatct gattttttga gttttcgtta tgaagggaag tcttttgaaa gaaaagatga
    36361 tagttcatta cacctgatta ctaacaaggt tctggcgtgt taccaacgcg aagctaacaa
    36421 ggaaatagcg cgtctattaa ataatcatca gaagttaaat aatc
//
LOCUS       DAZGBC010000029        36332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGBC010000029 DAZGBC010000000
VERSION     DAZGBC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.029"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            254..1627
                     /gene="aroP"
                     /locus_tag="ACHK1J_001498"
     CDS             254..1627
                     /gene="aroP"
                     /locus_tag="ACHK1J_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO7910795.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1670..2524)
                     /gene="ampE"
                     /locus_tag="ACHK1J_001499"
     CDS             complement(1670..2524)
                     /gene="ampE"
                     /locus_tag="ACHK1J_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO7910796.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2521..3072)
                     /gene="ampD"
                     /locus_tag="ACHK1J_001500"
     CDS             complement(2521..3072)
                     /gene="ampD"
                     /locus_tag="ACHK1J_001500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO7910797.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDISNNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3160..4053
                     /gene="nadC"
                     /locus_tag="ACHK1J_001501"
     CDS             3160..4053
                     /gene="nadC"
                     /locus_tag="ACHK1J_001501"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO7910798.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4256..4696
                     /gene="ppdD"
                     /locus_tag="ACHK1J_001502"
     CDS             4256..4696
                     /gene="ppdD"
                     /locus_tag="ACHK1J_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO7910799.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTIRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4706..6091
                     /gene="gspE"
                     /locus_tag="ACHK1J_001503"
     CDS             4706..6091
                     /gene="gspE"
                     /locus_tag="ACHK1J_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO7910800.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6081..7283
                     /gene="hofC"
                     /locus_tag="ACHK1J_001504"
     CDS             6081..7283
                     /gene="hofC"
                     /locus_tag="ACHK1J_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO7910801.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRFPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7318..8361)
                     /gene="guaC"
                     /locus_tag="ACHK1J_001505"
     CDS             complement(7318..8361)
                     /gene="guaC"
                     /locus_tag="ACHK1J_001505"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO7910802.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8517..8561)
                     /locus_tag="ACHK1J_001506"
     CDS             complement(8517..8561)
                     /locus_tag="ACHK1J_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910803.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8586..9206
                     /gene="coaE"
                     /locus_tag="ACHK1J_001507"
     CDS             8586..9206
                     /gene="coaE"
                     /locus_tag="ACHK1J_001507"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO7910804.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9206..9949
                     /gene="zapD"
                     /locus_tag="ACHK1J_001508"
     CDS             9206..9949
                     /gene="zapD"
                     /locus_tag="ACHK1J_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO7910805.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9959..10156
                     /gene="yacG"
                     /locus_tag="ACHK1J_001509"
     CDS             9959..10156
                     /gene="yacG"
                     /locus_tag="ACHK1J_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO7910806.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10372..10761)
                     /gene="mutT"
                     /locus_tag="ACHK1J_001510"
     CDS             complement(10372..10761)
                     /gene="mutT"
                     /locus_tag="ACHK1J_001510"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO7910807.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10821..13526)
                     /gene="secA"
                     /locus_tag="ACHK1J_001511"
     CDS             complement(10821..13526)
                     /gene="secA"
                     /locus_tag="ACHK1J_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO7910808.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13588..14100)
                     /gene="secM"
                     /locus_tag="ACHK1J_001512"
     CDS             complement(13588..14100)
                     /gene="secM"
                     /locus_tag="ACHK1J_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO7910809.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14331..15248)
                     /gene="lpxC"
                     /locus_tag="ACHK1J_001513"
     CDS             complement(14331..15248)
                     /gene="lpxC"
                     /locus_tag="ACHK1J_001513"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO7910810.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15349..16500)
                     /gene="ftsZ"
                     /locus_tag="ACHK1J_001514"
     CDS             complement(15349..16500)
                     /gene="ftsZ"
                     /locus_tag="ACHK1J_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO7910811.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16561..17823)
                     /gene="ftsA"
                     /locus_tag="ACHK1J_001515"
     CDS             complement(16561..17823)
                     /gene="ftsA"
                     /locus_tag="ACHK1J_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO7910812.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17820..18650)
                     /gene="ftsQ"
                     /locus_tag="ACHK1J_001516"
     CDS             complement(17820..18650)
                     /gene="ftsQ"
                     /locus_tag="ACHK1J_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO7910813.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18652..19572)
                     /gene="ddlB"
                     /locus_tag="ACHK1J_001517"
     CDS             complement(18652..19572)
                     /gene="ddlB"
                     /locus_tag="ACHK1J_001517"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7910814.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQADMSFSQLVVRILELAD"
     gene            complement(19565..21040)
                     /gene="murC"
                     /locus_tag="ACHK1J_001518"
     CDS             complement(19565..21040)
                     /gene="murC"
                     /locus_tag="ACHK1J_001518"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO7910815.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAKVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21094..22161)
                     /gene="murG"
                     /locus_tag="ACHK1J_001519"
     CDS             complement(21094..22161)
                     /gene="murG"
                     /locus_tag="ACHK1J_001519"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO7910816.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22158..23402)
                     /gene="ftsW"
                     /locus_tag="ACHK1J_001520"
     CDS             complement(22158..23402)
                     /gene="ftsW"
                     /locus_tag="ACHK1J_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO7910817.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23402..24718)
                     /gene="murD"
                     /locus_tag="ACHK1J_001521"
     CDS             complement(23402..24718)
                     /gene="murD"
                     /locus_tag="ACHK1J_001521"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO7910818.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADGRCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKISGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24721..25803)
                     /gene="mraY"
                     /locus_tag="ACHK1J_001522"
     CDS             complement(24721..25803)
                     /gene="mraY"
                     /locus_tag="ACHK1J_001522"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO7910819.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25797..27155)
                     /gene="murF"
                     /locus_tag="ACHK1J_001523"
     CDS             complement(25797..27155)
                     /gene="murF"
                     /locus_tag="ACHK1J_001523"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO7910820.1"
                     /translation="MISVTLSQLTDILNGELQGADITFDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27152..28639)
                     /gene="murE"
                     /locus_tag="ACHK1J_001524"
     CDS             complement(27152..28639)
                     /gene="murE"
                     /locus_tag="ACHK1J_001524"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO7910821.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     NDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28626..30392)
                     /gene="ftsI"
                     /locus_tag="ACHK1J_001525"
     CDS             complement(28626..30392)
                     /gene="ftsI"
                     /locus_tag="ACHK1J_001525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO7910822.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30408..30773)
                     /gene="ftsL"
                     /locus_tag="ACHK1J_001526"
     CDS             complement(30408..30773)
                     /gene="ftsL"
                     /locus_tag="ACHK1J_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO7910823.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30770..31711)
                     /gene="rsmH"
                     /locus_tag="ACHK1J_001527"
     CDS             complement(30770..31711)
                     /gene="rsmH"
                     /locus_tag="ACHK1J_001527"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO7910824.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31713..32171)
                     /gene="mraZ"
                     /locus_tag="ACHK1J_001528"
     CDS             complement(31713..32171)
                     /gene="mraZ"
                     /locus_tag="ACHK1J_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO7910825.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32773..33777)
                     /gene="cra"
                     /locus_tag="ACHK1J_001529"
     CDS             complement(32773..33777)
                     /gene="cra"
                     /locus_tag="ACHK1J_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO7910826.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33859..33945)
                     /locus_tag="ACHK1J_001530"
     CDS             complement(33859..33945)
                     /locus_tag="ACHK1J_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO7910827.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33957..34448)
                     /gene="ilvN"
                     /locus_tag="ACHK1J_001531"
     CDS             complement(33957..34448)
                     /gene="ilvN"
                     /locus_tag="ACHK1J_001531"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7910828.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34451..36175)
                     /gene="ilvI"
                     /locus_tag="ACHK1J_001532"
     CDS             complement(34451..36175)
                     /gene="ilvI"
                     /locus_tag="ACHK1J_001532"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO7910829.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRSNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36117..>36332)
                     /locus_tag="ACHK1J_001533"
     CDS             complement(36117..>36332)
                     /locus_tag="ACHK1J_001533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910830.1"
                     /translation="LSFVMEDFIVSFHLSSCLFLLPGVYVSGCQLLKQDRTVNVFYTF
                     FPSNSEAGHGDVVWSRDGRPIAYRSGR"
BASE COUNT         8689 a        10196 c         8924 g         8523 t
ORIGIN      
        1 tttaaattca ttctgcatat tgagaggggt tgaggctgag atttacaaac ggtttctttt
       61 taagcaactc atcttcaacc atgcataaag cgggtgcatt cgctgccgca taccattatt
      121 cttgatctga cggaagtctt tttgtaacaa ttcaaacttc tttgatgtaa acaaattaat
      181 acaacaaacg gaattgcaaa cttacacacg catcactgcg tagatcaaaa aaacaatcac
      241 cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc gcggccttaa
      301 aaaccgccat attcagctta tcgcgctggg tggcgcgata gggaccgggt tattcctggg
      361 tagcgcctcc gtaatacagt ccgcagggcc agggattatc ctgggttacg ccattgctgg
      421 ttttatcgcc tttctgatca tgcgtcagct gggtgaaatg gtggtcgaag aacctgtcgc
      481 aggctccttt agccactttg cttataaata ctggggcagc tttgctggct tcgcttctgg
      541 ctggaactac tgggtactgt acgttttagt tgccatggca gagctgactg ctgtgggtaa
      601 atacattcag ttctggtatc cggaaatccc aacctgggtt tctgccgccg tgttctttgt
      661 ggtgattaac gccatcaacc tgaccaacgt aaaagtgttt ggtgagatgg agttctggtt
      721 tgccattatc aaagttattg cggtagtagc gatgatcatc ttcggcggct ggctgctgtt
      781 cagtggtaac ggcggtccgc aggcaagcgt tagcaacctg tgggatcagg gtggtttcct
      841 gccgcacggc ttcaccgggc tggtgatgat gatggcgatt atcatgttct cgttcggtgg
      901 tctggaactg gtggggatca ccgcagcaga agctgataac ccggagcaaa gtatcccgaa
      961 agcgactaac caggttatct accgcatcct gattttctat attggttcgt tagccgttct
     1021 gctctcactg atgccgtgga cccgcgttac cgccgatacc agtccgtttg tgctgatctt
     1081 ccacgagtta ggcgatacct ttgtggcgaa tgcgctgaac atcgtggtgc tgactgcggc
     1141 gctctccgtg tacaacagct gcgtatattg caacagccgt atgctgtttg gtctggcaca
     1201 acagggtaat gcgccaaaag cgctggcgtc tgtcgataaa cgcggcgtac cggttaacac
     1261 cattctggtg tctgcactgg taacggcatt gtgcgtactg attaactatc ttgctccgga
     1321 atccgcattt ggcctgttaa tggcgctggt ggtatctgca ctggtgatca actgggcgat
     1381 gattagcctg gcgcatatga aattccgtcg cgccaagcag gaacaaggcg tggtaactcg
     1441 cttccctgct ctgctttatc cgctgggtaa ctggatctgc ctgctgttta tggcggcggt
     1501 actggtgatt atgctgatga ccccaggaat ggcgatttcg gtatacctga tcccggtatg
     1561 gctgatcgtg ttaggtatcg gctatctgtt taaagagaaa accgccaaag ccgtaaaagc
     1621 gcattaatct ctctacgccc tcacctgtac acggtgaggg caataatctt tacaccaacg
     1681 ccccgtaaat cgtcagtagc gcaatcacca ccacgaccac gaacgaggtt ttcttcgcca
     1741 ttgaaaccgc tgccttcggc gtctccacct tatcgacatg cggttcacgc gccagagaga
     1801 actgcgccag acgcgttaac acctgatact gcgaagtatg gaaatcaccc agcgaagcaa
     1861 accaggccgg taacgctttc tcaccatgac cgatcaaggc atataccaca cccgcaagac
     1921 gaactggcac ccaatccagt acatgaagca cggcatcaat gccggactgt aaacgatgat
     1981 gcggcgtctg gtatcgcgcc agccagtatt gccatgcacg caagaacgca taccccatca
     2041 gcgtaacggg tccccaggtt ccccccacaa tcagccagaa cagcggtgca agataaaaac
     2101 gaaagttaat ccacagcaat gcattttgca gctcacgcaa atactcacgt tcgtcgcagc
     2161 ctgccgggac gccgtgaatc atggtgagtt cgccagccat cgtggcacgg gcatggctat
     2221 cattacgtga agcagctgtc agataagcat gataatgaag acgaacttta cctgcgccaa
     2281 tacacagcaa accaatcagc agccacacca gcagcgtggg aacgttgaac aatacgccct
     2341 gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg cctaacgtgc
     2401 gcccgagaga aaaatgtttc acccgccgaa agaacgcttc aagacgatga tcaagctgcc
     2461 agtgctcgcc caacttaaac aggcgctcga aaattaacac cagtaaggtt gtaaatagcg
     2521 tcatgttgtc tccttgctga ccagcgcacg aaaccgtgcc caatcaaatg caggaccggg
     2581 atcggttttc cgatccggcg caatatcaca atggcccgtc atgttattag agatatccgg
     2641 atagcaatca atcagtgccc gcgtaaccgc cgcaagctgt tgatactgcg catcggtata
     2701 cgccagcgta tcggtgcctt caagctcaat cccaatagaa aaatcattgc agcgttcgcg
     2761 cccctgatac tgagagactc ccgcatgcca tgcacgttta tcgaaaggaa catactggac
     2821 tatttcacca tcacggcgaa tcaaacagtg agcggagacg cgcaaatggg cgatctcagc
     2881 aaagaaagga tgtgcctgcg gatcaatagt tccagtgaat aatgcgtcga tccacggacc
     2941 gccaaactcg cctggcggca ggctaatatt gtgcaccacc agcagggtgg gtgtttcgtc
     3001 atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca gccacccctg
     3061 ttctaacaac atgcaggatc tccttatatg tggtgctaat acccggttca gagtagcatg
     3121 tttctacctt atgattcgtt agctatctgg agttttaaca tgccgcctcg ccgctataac
     3181 cctgacaccc gacgtgacga gctgctggaa cgcattaatc tcgatatccc cggcgcggtg
     3241 gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg ccaacaatga cattacggca
     3301 aaacttttac cggaaaactc tcgctctcat gccacggtga tcacccgcga gaatggcgta
     3361 ttttgcggca aacgctgggt tgaagaggtg tttattcaac tggcaggcga cgatgtcacc
     3421 ataatctggc atgtggatga cggcgatgtc atcaacgcca atcaaccctt gttcgaactt
     3481 gaaggcccat cccgcgtgct gttaacgggc gaacgcactg cgcttaattt tgtgcaaacc
     3541 ctttcaggag ttgccagtaa ggtacgccac tatgtcgaat tactggaagg caccaacacg
     3601 cagttgttgg atacgcgcaa aaccttaccc ggcctgcgtt cagcgctgaa atacgcggta
     3661 ctttgcggcg gcggagcgaa tcaccgtctg gggctttctg atgccttcct gatcaaagaa
     3721 aaccatatta ttgcctccgg ctcagtgcgc caggcggtcg aaaaagcgtc ctggctgcac
     3781 ccggatgcgc cagtagaagt cgaagtagag aatctggaag aacttgatga agccctgaaa
     3841 gcaggagccg atatcatcat gctggataac ttcgaaacag aacagatgcg cgaagccgtc
     3901 aaacgcacca acggcaaggc gctactggaa gtgtctggca acgtcactga caaaacactg
     3961 cgcgaatttg ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac taagcacgta
     4021 caggcactcg acctttcaat gcgttttcgc tagttgctga cagatttatc cgctccatag
     4081 tgggcggata atcacccctt ccccaacttt cttcgtaacg cctcgcaaat ttcttgtctt
     4141 tcgctgcaac ggaataaaaa acctcggaag ctgctttccc gtttcttttt ttgcccctcg
     4201 cttgtgctga tggcgtttgc caaagtagcg ccaacccaat caaggagcga aacagatgga
     4261 caagcaacgc ggttttacac ttatcgaact gatggtggtt attggcatca ttgccatttt
     4321 aagcgccatt ggtattcccg cttatcaaaa ctacctgcgc aaagccgcac tcaccgacat
     4381 gctacaaacc tttgtgcctt accgtaccgc cgtagagttg tgcgcgctgg aacatggtgg
     4441 attagatacc tgcgacggtg gcagcaatgg cattccctcg cctaccacca tccgctatgt
     4501 ttcagccatg agtgtggcaa agggcgtggt gtcgctgacc gggcaagaaa gtctcaatgg
     4561 gctaagcgtc gtcatgacgc caggttggga taacgcaaac ggcgtcaccg gctgggcgcg
     4621 caactgcaat attcaaagtg acagcgcatt gcagcaagcc tgcgaagatg tcttccgctt
     4681 tgatgacgcc aactaaggag cggcaatgaa tattccacag ctcacggccc tgtgtctgcg
     4741 ttatcaggga gtcttgctgg atgccagcga agaagtggtt catgttgcgg tggtcgatgc
     4801 cccctcacat gagttgctgg acgcattgca tttcgctacc accaaacgta ttgagatcac
     4861 ctgctggacg cgccaacaaa tggaaggtca cgccagtcgc acacaacaga cattgcccgt
     4921 agctgttcag gagaagcatc agcccaaagc agagttgctg actcgaacgt tacaatctgc
     4981 gctggaacaa cgcgcgtctg atattcatat cgaaccagcg gacaatgcct accgcatccg
     5041 cttgcgtatc gacggcgtat tgcatccttt accggatgtt tcaccggatg ccggagtcgc
     5101 attaaccgcc agattaaaag tgctgggaaa cctggatatt gcggaacatc gcctgccgca
     5161 ggacgggcaa ttcactgtcg aactggcagg aaacgccgtc tcatttcgta ttgcgacctt
     5221 accatgtcgg ggtggtgaaa aggtggtatt aaggttgtta cagcaggtga gccaggcact
     5281 ggatgtcaac acgctgggaa tgcagccgtt acaactggcg gactttgctc atgccttgca
     5341 acaaccacag ggactggtgc tggtaactgg ccctaccggc agcggcaaaa cggtcacgct
     5401 ttatagtgcc ctgcaaacgc tgaataccgc tgacattaat atttgtagcg tcgaagatcc
     5461 ggttgagatc cccatagccg gactaaacca gacgcaaatc catccgcgtg ccggactcac
     5521 ctttcagggc gttttgcgtg cgttattgcg ccaggatcct gacgtcatca tgatcggaga
     5581 gatccgcgat ggcgaaacag cagagatcgc tattaaagcc gcgcaaaccg gtcacctggt
     5641 gttgtctacc ctacacacca attccacctg cgaaacgctg gtacgtttac agcaaatggg
     5701 agtcgcccgc tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg
     5761 taaactttgc ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt
     5821 atggccgtcg ccgctgcccc actggcaggc acctggttgt gtacattgct accacggttt
     5881 ttatggtcgc acggccttat ttgaagttct gcccataaca ccggtcatac gtcagcttat
     5941 ttccgctaat accgacgttg aatcgctgga aactcacgcc cgacaggcgg gtatgcgtac
     6001 gctttttgaa aacggctgcc tcgccgtgga gcaaggctta actacctttg aagagttaat
     6061 ccgcgtactg gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg
     6121 gcgacggcaa tgcgcaagat gggatgctat gggcagagag ccgtacttta ctgcttatgg
     6181 cactacagca acagatggtt accccactaa gcctgaagcg aatcgccatc aattctgcgc
     6241 agtggcgggg agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagcag
     6301 ggttaacgct ttctgaaggg ctggctctgc tggcggaaca gcatcccagt aagcaatggc
     6361 aagcgttgct gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct
     6421 tattaccctg gtcagaggta tttccgccgc tctatcaggc gatgatccgc acgggtgaac
     6481 tgaccggtaa gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc
     6541 agttgaccga caaagtgaaa tcagcgttgc gttatcccat catcatttta gcgatggcaa
     6601 tcatggtggt tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atatataaga
     6661 ccttcaacac cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg
     6721 gcgaatggag ctggctgctg gtgttgttcg gcattatgct gacgatagcc aataagttgc
     6781 tgatgcgccg accgacctgg cttatcgcgc ggcagaaatt gctgttacgc ttcccgatta
     6841 tgggttcact gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgactc
     6901 aaagtgcagg cattactttt ttacagggcg tagagagcgt cagagaaaca atgcgctgcc
     6961 cgtactgggt gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct
     7021 ggctggcgct aaaaaatgcc ggtgagttta gcccgctctg tttgcaatta gtgagaacgg
     7081 gagaggcctc cggctcgctg gatctcatgt tagacaacct cgcccatcat catcgggata
     7141 acacaatggc gctggcggat aacctagcag ccttactgga accggcgttg ctgatcataa
     7201 cgggaggaat tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg
     7261 atgcgatgag tgggatggga taacgctggc gtgttgctcc acaccagcgt tggaagatta
     7321 caggttgttg aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt
     7381 cagttctttc aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat
     7441 atcacgcgcg gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc
     7501 tgcgcgatat tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc
     7561 gtagaacagc ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg
     7621 gcccgccagc atgccgccaa gcatgacgaa atcggcaccg ccgccaaaag ctttcgcgac
     7681 atcgcccggc gtggtgcaac caccatcgct gacgatcatt ccgcccagac cgtgcgcagc
     7741 atcggcacat tcgattaccg cagaaagttg cggataaccg acgcctgttt tgacacgagt
     7801 tgtacaaaca gaacctgggc caatgccaac tttaacgata tcggcaccgg agaggataag
     7861 ctcctcacac atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc
     7921 ttcacgcgct ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat
     7981 acaaacgaag tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc
     8041 atcagacgta ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat
     8101 aaacgcttgc cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga
     8161 agccagcgca gaggccatag aaaatgtgcc tacggtgtcc atatttgcgg cgataatcgg
     8221 cacgccggac cagctctgac ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc
     8281 ggaacggctt ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt
     8341 cagatcttct tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc
     8401 gcaactccag tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc
     8461 tttttttacg ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa
     8521 tttcgctttc gtgcgctcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat
     8581 aaattatgag gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg
     8641 ccaatgcgtt tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg
     8701 tggttgaacc aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga
     8761 ttgctgctga tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg
     8821 aagagaaaaa ctggcttaac gacctgctgc atccgctgat tcagcaagag acgcaacacc
     8881 agatccagca agcaacctcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact
     8941 cactgtataa aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta
     9001 agcgcaccat gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc
     9061 aggcaacgcg cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac
     9121 cggatgctat cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc
     9181 agtttgtctc acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa
     9241 tgaaaaaatg cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt
     9301 acccatcgtt gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga
     9361 tgttttcgag cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg
     9421 taaactccag acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat
     9481 tcagcagtta aaagcggcgg ggagcgtatt aatctccgcg ccgcgtatcg ggcaatttct
     9541 gcgtgaagat cgcttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg
     9601 cagctttgat ttaccaacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag
     9661 ccaggtagaa acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct
     9721 ggatttaatt cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca
     9781 ggataacggt ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta
     9841 tccgcaaatt tccggtcata agagccgttt tgccattcgt tttatgccgc tggacagtga
     9901 aaacggacag gtaccggaac gtctggattt cgaactggcc tgttgctaag gagtaaaaat
     9961 gtcagaaact attacggtga attgcccaac ctgcgggaaa acggtggtgt ggggtgaaat
    10021 cagcccgttt cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc
    10081 tgctgaagaa aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga
    10141 agaaccaaag cagtgacatt tgtctgatgc cgcacgtagg cctgataaga cgcggacagc
    10201 gtcgcatcag gcatcgtgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca
    10261 aaaaaaaatt tatcaatgag ttagaaatcc ctttaggcct gataagacgc gccagcgtcg
    10321 catcaggcat cgtgtgcaaa tgtcggatgc ggcgaaaacg ccttatctga tctacagacg
    10381 tttaagcttc gcaattaccg gttcattggc tggcggaaaa tcatcggcat taagaccgac
    10441 tagcgacatc cactcaccgg gttgcccttc tttaccccac ggctcccctt cccagctttc
    10501 gaccagccag aaccacagag ttatatgcct gtccgggaat tcatattcca gtttttcaaa
    10561 tagcgaaaaa tgttgtgggg taatcccgac ttcttcctga agttcacgca ccaccgcctg
    10621 ttccggcgtt tcacccattt caattttacc gccgggaaac tccagtttat tcgccatgtg
    10681 cgcatctgct gcgcgacgcg ttataaagat ttcattgttc tcgttgcgaa taatacctac
    10741 cgcaatttgc agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct
    10801 tttacttcaa ctgttagctt ttattgcagg cgaccatggc actgcttgta ttttttaccg
    10861 gaaccgcacg ggcaaggatc gttacgtcct actttgcgtt caccggtttg cgccgccagt
    10921 gcagctgcgg ctgcagagtc gtcatcctga tggctaagct gctgcatttg cgctaaacgc
    10981 tcggcttcca tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga
    11041 actttgctca gcgtactgat aacttcatat ttcaacgact ccagcatcgc tgcaaacatg
    11101 gagaacgatt cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg
    11161 ataccctgac gcagatagtc catcgctgcc aggtgctctt tccacaggga gtcaagcgtt
    11221 tgcagcatga cgcctttctc gaagtgacgc atcatctcag caccaaccac ttcttcttta
    11281 cgctgataca cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt
    11341 tcgggttctt tatccagcca ctcagcaatt ggtaaatcga ggtcgaaatc gttcttcaga
    11401 cgttcctgca gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca
    11461 tcaatggtgg ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg
    11521 acatccagca gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca
    11581 tcatattcca gcagttgctt acgaatgtcg aagttacgac tttcaacttt acgctgggcg
    11641 ttggcaatcg ctttagtcac ccacgggtgt tcaatggctt cgcctggctt catacccagt
    11701 ttacgcatca ttccagatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc
    11761 gacaggtaga aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg
    11821 ttatcgatac gacgggattc gtgacgctcg gtaccgataa tatgcaggcc acctgctgcc
    11881 agtaccgcat cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc
    11941 ggattttcca gcgcggcaac ttctgcctgc cagctaccac cgagcacaat atctgtacca
    12001 cgacccgcca tgttggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc
    12061 gccgcttcgt tggcgtggaa tttggcgttc aggacattgt gcttaatacc ggctttggtc
    12121 agttcgtttg acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg
    12181 cctttcgcag tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc
    12241 atgtagacca ggtcaggcag atctttacga atcattggac ggttggtcgg aacaacgacg
    12301 gtatccagtt tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc
    12361 cccgccagtt tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg
    12421 ttttcgttct ggatctgcac accttctttc gcttccacag cctggtgcag accatcagac
    12481 cagcgacggc cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct
    12541 ttaacgatgt agtcgacgtc acgggtaaac agcgcatgag cgcgcagcgc cgccgttacg
    12601 tgatgcatca gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc
    12661 tctttcacca gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga
    12721 gatttttcgt ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc
    12781 tgacggatca ggtgcggaat aattttattc acgcgtttat acatttccga gctgtcttct
    12841 gccgggccgg aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg
    12901 tccaccagcg catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatgttg
    12961 tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa
    13021 gcttcgcgct ttgccggtgc tggcatgccc ggcaggttga taccgacagt caggccaagg
    13081 aattcaaaca gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta
    13141 actacatgca cgcctttacc ggttagtgcg ttcagataag caggcagcgt tgcggtcagg
    13201 gttttacctt caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg
    13261 agtaactgaa cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgtaccacg
    13321 gcgaaagctt ccgggatcag attttccagc acttcgcctt tttccagacg tgcacgaaac
    13381 tctgcggttt tccctttcag ttcttcgtcg gagagttttt ccatctccgg ttccatggca
    13441 ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa
    13501 actttagtta ataatttgat tagcataata aaatctcaaa cgccccgcgt tgcggagtta
    13561 ataaaatgaa gtaaaggttt attgttgtta gctgaggcgt tgagggccag cacggatgcc
    13621 ttgcgcctgg cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata
    13681 atcaatgcga taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt
    13741 atccagtaat gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagcgt
    13801 ttgcggtgcc attgcgaagg aaagatgacg gattaccgtg cgaatggcat gttgatgcca
    13861 gtaatcaacg gaatagttcg aattcgggcg gcgcgtgttc gcttccagca aggccaattg
    13921 accaaagtta actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt
    13981 tggttcggcg gcgttgctga gcgcaggcaa acctaaactc gccgcaacca tccctaataa
    14041 gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac
    14101 gttattgcca tcccgatcgt attcaaacgt atttagcgca caaatcttat cattaaagcc
    14161 tgaagtccac agcacgaatg aagataagtt gtccgtaaaa gtgcttaaga aaacagcgtt
    14221 cgcaccgttt tttatagctc ttataaaatt caaaaaaaaa cgactggttc acctggccgg
    14281 agagagtgcc agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt
    14341 acagctgaag gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat
    14401 tcccaggctt cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg
    14461 gatttataag cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc
    14521 gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt
    14581 acgcgataat cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg
    14641 gactgcagat attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc
    14701 ataaacgcat cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga
    14761 tggttaaaat cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac
    14821 ttatcgccat cttcgacacg aacagtctct ttgatgcgaa caaatttctt ggcgcagttc
    14881 aactcgtcga taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg
    14941 atcgggattt ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga
    15001 gcagcattga ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta
    15061 cagagcatgg tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag
    15121 tcggtgcgac gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagggtgact
    15181 ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt
    15241 ttgatcatcg tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc
    15301 gggccagttt agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg
    15361 cttacgcagg aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt
    15421 gtcattcacg actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccgtgctg
    15481 ctggtagcga tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg
    15541 acgtttgtcc atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc
    15601 cgggtcaaga gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt
    15661 gttacctacc gtttcgaact catccagacg caggtcgaag cccgccgtga tgttaaccag
    15721 tacgccgcgc gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc
    15781 agcagcttct tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc
    15841 catttcagac attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt
    15901 aatcagttcg gcgatacctt gcacagcgcc tttgagtaca tcgttcgctg cgccaaacgc
    15961 atccagcagg gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat
    16021 cagagagtcc acatgcttgg acagttcagt gatcccctgc tccgcgaatg ccatacgctt
    16081 cttgccttca aagttgaagg gcttagtgac gacagcaacg gtcaggatac ccaaatcttt
    16141 tgccacttca gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc
    16201 aataaagacc atgtctgcac cttccagcgc cgcacgcagt gcatcgcgat cctcatcagc
    16261 cgcattgcgg ccaacttctg gattagcgcc agcgcccagt cctttggtga taccgctacc
    16321 gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc
    16381 gaagaattca acaccttcaa tgcgctcgcg caccatgtgt tcaacagcat taccgccgcc
    16441 gccgccgacg ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat
    16501 agtttctctc cgatttgtgc ctgtcgcctg aggccgtaat cattgtcggc ctcataaaaa
    16561 ttaaaactct tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt
    16621 aacacgtttt tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa
    16681 tcccaccgcc gttgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag
    16741 cggcgcgccg atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc
    16801 gatctgcgct gcgccaccag ttaatacaat gcctgccgcc aggtgatgtt taaccccttg
    16861 ttggcgaagc ttttcctgca actgcaatat ctcttcgttg accaggttga gcagctcggt
    16921 atagcgcggc tcgatcacct ctgccagggt ctgacgttgc agactccgcg gcggacgacc
    16981 gcctacgctc ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc
    17041 gtggcgaact ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc
    17101 actggtcacg acattgccag cataaggaat taccttagtg tggcgcaatg ccccaccggt
    17161 ataaacggcg atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg
    17221 ttcatcttcc gtcaataccg aataacttga tgccagaccg gcaaatatca gttggtcaac
    17281 tttcagccca caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtggcatgt
    17341 gatcaggtgc acttttgcct gcatccgcac gcccgaaagt cctaccggat tcttgatccc
    17401 ttcctgatag tcaatcgcat actcttgcgg gatcacatgc agcacacgat gctcatcgcg
    17461 cacacgcacc gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc
    17521 ttcagaaata ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc
    17581 cagatatacc gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgttg
    17641 tacgcacttg accacggatt cgaggtcgtt caccccgcct ttatccatac cacgcgacgg
    17701 gcagctgccc acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc
    17761 ggcaaccttc gcggtaccaa tctccagtcc tactaccagt tttctgtccg tcgccttgat
    17821 cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc
    17881 gcccagccta ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca
    17941 tcggtttgcg cctgctgctg taaaaccgga taaagttcta caaagcgagc caaacgtttc
    18001 atcgtatcgc cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa
    18061 cgccgcgcgg tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc
    18121 atttcgcgat agccctgcaa cacttcattg gcgctgcctt ccgggccata cagcattgga
    18181 agcacctgct tgctggtgcg atctggcggc acgctgaagg tatttccttc cgcgtctacc
    18241 atgtgttgat cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca
    18301 tcaggccact gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc
    18361 gtctggatga tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc
    18421 gactgccgga tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag
    18481 agcggcaggc gttgcgcatc ttccatccag cccaacacga cccagccgct caccaacact
    18541 gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga
    18601 gaagaaacct cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc
    18661 cagttccaga attcgtacta ccaactgcga gaagctcata tctgcctgac gtgccgccat
    18721 cggcaccagg ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg
    18781 tccatcactg tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca
    18841 tgctttcagc actaatgcct gcaaattggc ctcttgtgac gcttccagac ctgcggggca
    18901 gaaatactgt gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg
    18961 ttgaatacgt attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc
    19021 acttagccat ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc
    19081 ttgtagagca ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg
    19141 cttaacgata accggcaaac ccagagcaga aatttctgct aactgcttat cgctaaggcc
    19201 tttttcaaac tctgcgcggg ttaacgctac ccacggtgcg accggtaaac cagcaccttg
    19261 ccacagcagt ttgctgcgta gtttatccat tgaaagcgca gatgccatca ctccgctgcc
    19321 ggtataaggc aagcccatca gctcgagcat cccctgcagc gtaccatctt caccgccgcg
    19381 gccgtgtagc gcgataaaca ctttctgaaa gcccatcgac ttcagttgcg tcacgtcgac
    19441 ttctttcggg tcgacaggat atgcgtcaat accgccttca cgcagcccgg ctaacaccgc
    19501 tgcgccagaa ttcaaagaaa cttcccgctc agcggaggtc ccgcccaaca ggaccgcgat
    19561 tttatcagtc atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag
    19621 aacgggcaat ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta
    19681 ataccggtgc cagcatctcg gctaccttcg ccggatccgg caccagaatg ggatctattt
    19741 tcccacgtcc acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt
    19801 cgccagccgg atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga
    19861 aatcatcata caggtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt
    19921 ttttatccgg ccagcctgca cgcgccgctt taatagtggc gtccacttcc gtcgggtggt
    19981 ggccgtagtc atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga
    20041 attcaccaag gaaatcaaaa cggcgaccag tcccctggaa gctctccagc gcacgcaaaa
    20101 tagcctcgtc gtcaatgccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg
    20161 cgttgtgacg acctggtgca ttcagggtga cgcgcatcgg ctctttgtcc tggcgcagca
    20221 gcgtaaagtg cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt
    20281 cgctgaagcc gtaagttgtg gtctgacgtc ccactcgcgg taacaattcg cggatcaccg
    20341 gatcatcaac acacatcacc gcacgaccgt aaaacggcag gttgtgcaga aaattaataa
    20401 aagtctgttt taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat
    20461 tggtgacaat cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt
    20521 cggcaatcag gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc
    20581 cgccgttaac gaaggttggg tcgagccccg cttctgcgta gatgctggag accatcgcgg
    20641 tagttgtcgt tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact
    20701 cagccagcat ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt
    20761 ccgggttatc ggcagaaata gcgctggaaa caacgaccac gctggcatca cgtacgtttt
    20821 ccgggcgatg gttgaaataa atcgtcgctc ccagattcat taactgctgc gtgaccggat
    20881 ttggcgctaa atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac
    20941 cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg
    21001 gcacgatgga acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct
    21061 taaattcata cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc
    21121 atttgccact cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt
    21181 taataaggtt tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg
    21241 tggctgctcg ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg
    21301 ctggcggtct ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc
    21361 actcaccgtt aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc
    21421 catatcatca ataaattcag tcactttatg ctgcggttgc cccgcttcgg catacgcctg
    21481 ttcaacggat tgttgcgaac ctttgccgct ctgatgccag atagtgactg aatcacccag
    21541 tttcgcagca acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac
    21601 taccagcaca cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggtagcgc
    21661 caacacatcg gtacgcaccg ggttacccac cacttccgca ttggggaaag cacctggaaa
    21721 cgcctgcatc actttggtgg caatcttcgc cagccattta ttggttaagc ccgcaatacc
    21781 gttttgttca tgaagtacaa ccggaatgcc taacgaccac gcggccagac caccaggacc
    21841 tgatacgtag ccgcccatac cgagcaccac gtcaggtttg tacgctttca taatcgcccg
    21901 cgcctgacgc caggcattga agatacgcag cggcgcagct atcagtgctt ttataccttt
    21961 tccacgcaga ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc
    22021 cgcttccata cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagatg
    22081 gtgcgcaacc gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa
    22141 tcgctttcct tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc
    22201 gtttcataat caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa
    22261 ccaccgtaac tgatcagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg
    22321 cctacgttaa ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg
    22381 gaaaaacggt ggtcaatttc taatgcttta cggccaatcg acatcgcgcg aaaagcgacg
    22441 aagaatacca ttaaaagtgc cagcaccaca ccgacatacc ccagttcttc gccgataatg
    22501 gcgaaaataa agtcagtgtg cgcttccggc agatactcca gtttttgtac cgagttacct
    22561 aaaccttgtc cccacagttc gccgcgacca aacgccatca gcgattgcgt taactgatag
    22621 ccgctgccaa agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac
    22681 ggttcggcga ggatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac
    22741 tgccacaatt tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc
    22801 accgtaccaa ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc
    22861 ggtttcagga agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg
    22921 gcgatatagc aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg
    22981 agatcgatcc aacgcgatgc ccctttaacc gagctcccca ctaccagcac gatcatcagc
    23041 aggatgatag atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga
    23101 cgcagcgtaa tgatcgccag aataaacgcc aaaatcagat agacaccatc acgcttcgcg
    23161 aagaagaacg gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg
    23221 ataaagccaa tcgccgcgag gccgaaggtc agccacagta aggtgcgatc gtacatgatc
    23281 aggctgtcgg tatctttttc ccgcgagccc atcacccagc cctttagcgc cgtggagatc
    23341 cagaccagga tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc
    23401 atcaacctaa ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga
    23461 actgatcaag gctggcacag gccggggaga gtagaaccat atcgcccggc tgaacacgcg
    23521 gagccagcaa gcgcatcgcc tgttccatag tttcggtttg ttctgccact tccgggcgta
    23581 gcgccgccag ctgcacgccg tcacgaccga aacaatacag acgtacgtta tcgccattca
    23641 ggtaacgcgc cagtgggcta aagtccgccg atttaccatc gccacccagc aacaaatgca
    23701 gcgtgccgtc tacgtgcagg ccattcagcg ccgcttccgt actgccgacg ttggtcgctt
    23761 tcgaatcgtt aatccaacgt acgccgttat gctccagcac aacttcaaag cgatgcggca
    23821 gaccagtgaa tgtggttaac gctttcaggc tgctggctcg cggtaaccct gcggcatctg
    23881 ccagtgccag cgccgccagc gcattggtgt agttatgctg cccggaaatt ttcatttctt
    23941 tcacgttcag cactttctcg cctttcaccc gcagccaggt ttcgccctgc tgatgattca
    24001 ggtgatagtc acccatgttg acgccaaagc tgacgcagcg tccatccgca ccgcgaatcg
    24061 gcattgttaa ggcatcatca gcattaacca cgcaaacttt cgcgttttcg taaatgcgca
    24121 gttttgctgc acgatactgt tgtaaaccaa acggatagcg atccatatga tcttcagtca
    24181 cgttcagaat ggtcgctgct accgcctgta agctggaggt ggtttccagc tggaagctcg
    24241 acagttccag cacgtacagt tcacactcat catccagtag catcaacgca ggcaggccaa
    24301 tattgccacc cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg
    24361 tgaccgtgct tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcgac
    24421 agaacagctc gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg
    24481 gatgcgccag tgcaataccg ggactggcga caatcagatc tgccgccatc agccattcat
    24541 cattcagact gcccgtgtgg cgttcaacgg cttcgggtaa tttatccagg ccaggcggtg
    24601 tcatacgcgt atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg
    24661 aaagcccggt gaggcccagg ccgataatga caacattttt accctggtaa tcagccatga
    24721 ttaacgtacc ttcagcgttg ccagaccaat caggaccagc atcagcgaaa taatccagaa
    24781 acgcacaatg acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc
    24841 catgcggaaa atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag
    24901 cgtttctacc acgaacacgc cccccataat caccagcagg aattcctgac gtagcagtac
    24961 ggcgataatg cctaacgcac cacctaacgc cagcgaacct acatcgccca taaagacctg
    25021 cgccggatag gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg cggtacagac
    25081 aataaccagt tccccggcgt gtcgcagata cggtatgtgt aaatagctgg caaagttcat
    25141 gttgccggtc gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat
    25201 tgccagaccg tcaagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa
    25261 gtaagccagc aaaatgtaga acagccccag ttgcggcatc acatctttaa agaatgggac
    25321 caccagctgc gttgcgggcg tgtctttgcc aacaaggtac agcgcgaagg cgacacccag
    25381 cgcaatgacc gacatccaga aatacttcca acgagcgatc aaccctttgg tgtctttacg
    25441 cactactttg cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa
    25501 cacgcaccag acgtacggat tggacgggta agcccacagc agtacggaga tcacaatcgc
    25561 cgtcaggatc ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg
    25621 accgtcgtta cgcaccacct gaccaaagga aagtttttgc aaatgagcaa tcatacgcgg
    25681 gcccatccac aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag
    25741 ataggaaaag acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa
    25801 catgtcccat tctcctgtaa agcgcgtact acctcttcca tggcggcact acgtgaaccc
    25861 ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac
    25921 gcagttttat cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt
    25981 ttacccacgc ttaacacgcg gtcaatacca gcagcttttg ccgcctcgcc cacctgtaca
    26041 tggcaggctt cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag
    26101 cccggcattt cagccagtac ctggactgct gcggtcattg aaccgacatt ggcgttgtag
    26161 gagtcgtcga gcagcaactg gttttctgcc agttggatgg ggaacagacg gcctggaaca
    26221 gctttcagat ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag
    26281 agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca
    26341 tccacgctac cggttggggt ttgcagggta aattccgtac cgtgcgaagt cacatggata
    26401 ttggtggcgg tgaaatcgct gttggcggca ttaggtgaga aacgccacac tttgcgtgag
    26461 ccaattacgc tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg
    26521 ttttccggca ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca
    26581 aaaccttcca gatgcgccgc tgccaggttg ttgaccagcg cagcttccgg gcgagtcaga
    26641 ctcacagtcc aggcaatttc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg
    26701 tattccggcg ttaagcgcaa cagcgtcatc ggtacaccga tgtcgttgtt gagattgcct
    26761 gccgtataaa gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag
    26821 gttttgccgg aggaccccgt cagagcaacc acgcgcgccg gaacttgctg gcgaacccat
    26881 gcagccagtt caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgatg
    26941 tccagcggac ggctaaccag tagtgcgccc gcgccgccag ctttcgcctg gtcggcaaaa
    27001 tcatgagcat cgaaacgttc gcctttcagg gcaacaaaca ggcagcccgg cgtcagtttt
    27061 cgggtatcag tggttacagc atcaaaggtg atatctgcac cttgcagttc accgttgaga
    27121 atgtcggtaa gttggctaag ggttacgcta atcatgcaat cacccccagc agacgcgcca
    27181 ccgtgacgcg atcggagtag tccagacgct gattgccaac aatctggtaa tcttcatggc
    27241 ctttgcccgc gaccagcacc acatcgttct ctttagcctg cataacggcg caagtcaccg
    27301 cttcagcacg gccttccatc actttggcat gtccggcatc taacattccc gccagaatat
    27361 cgttgatgat ggcacgcggc tcttcggtac gcgggttatc gtccgtcacc accgccacat
    27421 cagcaaactc ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac
    27481 cacagccaaa gacacaccac agcttgcccg cacagtgcag acgcgccgcc tgtaaggctt
    27541 tttccagtgc atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcgcag
    27601 tgaacacttc catacgtccg caaaccggtt gcagacgcgc ggcggttttc aacagatccg
    27661 ccaatggata gcccagtgcc aacagcgtcg ccagcgcgag cagcaggttg ctgacgttaa
    27721 aagcgcccat cagatggctc tcaatttcgc catcgcccca acttgagcta aagcgaatcg
    27781 tcgcaccgct gtcgtgatag ttcacgtcgt tcgcttttaa ccagcgtccg tgacagttcg
    27841 gattgatatg atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc
    27901 ggcccacttc atcgtcggcg ttaataatcg cctgaccgca atgatgctca gaataaagca
    27961 gccatttcgc ggcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta
    28021 agttggtaaa gaccgacgcc gcaaatttca atgccgccac acggtgctgt accagcccgt
    28081 gggaggaaac ttccattgcg caaaacgtcg cgccctgatc caccagcccc gccagctcgt
    28141 gctgaacatc gactgccgaa ccggtagtat tttctgtcgg gatcactttc cccagcagac
    28201 cgttaccaac ggtacccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca
    28261 gcagctgggt agtcgtggtt ttgccgttgg tgcccgttac gcctacgaga cgtaaattgt
    28321 cagagggttc atggtaaaag cggcccgcca gtgcagataa acgctcattg agctggctga
    28381 gatagatgac cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg
    28441 cctctgcaat aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt
    28501 ccgcctgatg acctactaca gctacaaaga gatcgcccgc cgcagccaca cggctgtcga
    28561 gtgtcatctc tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc
    28621 gcaaattacg atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg
    28681 cccgttgtca gcgcatccgg ctcgatgttc atggtacgca atacgccgcc catgatggca
    28741 ccaaagaccg gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata
    28801 acaacaacca gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata
    28861 tatttattga tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg
    28921 atacgatagc ctttaatcgc cgccttcacg ccgccgccgc ccggtagcgc cacgctttcc
    28981 atcatatgca ccacagtgcg gacaatggat tccgggaaga cacgttcacc gggaaccggg
    29041 gggtcaactt tggtaatcga cagcgggcga taaatgccgt agctgccgat agttgcgtag
    29101 actcgcgcta actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc
    29161 tctatgtcag accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc
    29221 aaattggtcg cttttcccag tccaaaacgt gagtaagtat ctactaacgc tgaggacggc
    29281 atcgctaacg ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg
    29341 gttaattcgc tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaatg
    29401 gtattgagta ccgagttttc ccgcaccacg ccacgttgca acgcggtcat caccaccatc
    29461 ggtttaaccg ttgagcccgg ttcaaacacg tcggtgatgg tacggttacg catcgcctct
    29521 tttggcgtgc cgctcagatt atttgggttg tatgatgggc tgttagccat cgccagcact
    29581 tcaccggtgt tgacatccac cagcacggcg ctaccggact cagccttgtt aaaggccacc
    29641 gcgttattca gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg
    29701 ttgtgcgcag cctggctgtc agtagaagaa atatcttcaa ttacgcgacc atagcggtct
    29761 ttacgcacaa tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcgacg
    29821 ccctcaatcc cctgactatc gacgttagta aagccgatga ggtgagcagt cacttcgccg
    29881 gatggataat aacggcgaga ttcttcacgc agatgaatcc ccggcagttt cagttttttg
    29941 atgtagtccg ccatgtcagg gtttacctga cgcgccagat aaataaagcg ccctttcggg
    30001 ttggcgttaa tgcgggctga aagctgatcc agcggaatat tgagcgcgtt agccagcgcc
    30061 ttccagcggt caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa
    30121 atcgctttta ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg
    30181 cgggaggtgg aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt caccagcata
    30241 tccggggaga taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga
    30301 atacagccgc ataacaacgc aaaacgccaa ctgataaagt tggcatgttc ttcctgacgt
    30361 tttggtttct gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact
    30421 acgatatttt cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt
    30481 tccacccggc tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg
    30541 tctaaagcat ctcgctccag caccagttgt tcgcgctgag cggtcagtaa acgggtatgg
    30601 tgcgccgtgg ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc
    30661 agcttcccaa atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg gcgctcgtgg
    30721 cttcccatcg atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg
    30781 tcctctctgc aatacgcaga actgaactac gggcacgagg attctcagcc acctcttctt
    30841 cgcccggcat taacttgcct agtgctcgca gctgacggcc acccagtttt ttgagctgct
    30901 cttcagtcat cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac
    30961 gtttcacaat acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccggtg
    31021 ccagcacgtt gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc
    31081 aaatgcgcac cgcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg
    31141 ttgcagcagc cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac
    31201 gctcgacaat ggcgcgggca atgcgtttgg caaaacgctc ttcaccatag gttttcaata
    31261 cccaggcgat atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg
    31321 ttgggtccat acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg cgttcagcat
    31381 catcaagttg cggtgaagag acgccaagat cgaggagaat gccgtcgatc ttgccgataa
    31441 gatcgcgctc ggcaacgtat tcgcccagcg cggaaaaagg tccgtggatg atggagaagc
    31501 gcggatcatc aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca
    31561 aacgcccctc ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag
    31621 tcccatcaat gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccaaca
    31681 gcaccgtagt atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc
    31741 gataagtctc cggtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccag
    31801 gttgtttcat cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc
    31861 agcccggcat gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga
    31921 cattcgctgg catgacctaa cagtaggcgc tgcacacggc gctcaaccgg gttcatgctc
    31981 gacagacgcg ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg
    32041 cacgggtgat gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt
    32101 tcccgataac gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct
    32161 ccccggaaca tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa
    32221 ggagtttacg gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa
    32281 aacccgatgt ttacggctaa tatcagcgtt gtttatttta cgtccaacgt gcgaagcaaa
    32341 ttaacgttat gaatagctgt aagaaaaaaa ccctcatcgt catcaccgtt ttaaaatgac
    32401 tcactatttt ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac
    32461 atatcttccc cggttaacag tccctgttgc gtctgtgtag cgcttgatgc cagcgggttt
    32521 gcgcaaaagc aaagaaaagt gcttgttaaa tcggcaattc atctctgcat gtaacaaaat
    32581 aatgcaaaaa cgcgctgtcg atatcactta attattgtga atttcagctg gtgagttaat
    32641 taacgggcgg gtttaaaagc atttatcaat aatagtcagg aatagtctta tttactttaa
    32701 ggatattgat gtccagtccc gtactctacg cgccagagtg aaattcacct ggcgcgtatt
    32761 tttgttcgca gcttagctac ggctgagcac accgcggcga tagagattac gtttaatgcg
    32821 cgttaaacca ggttttggct tacgcggttc gtccaggctt gccaggacaa tctccagcac
    32881 acgctctgcg acatcgcggt gacgttgagc cactgccagc accgggcact gtaagaagtc
    32941 gagcagttcg ttatcgccaa aggtggcaat tgccaggtca gaaggcagtt taccatcgcg
    33001 acgtagcgtg acatccatca ccccttgcaa caacgcaaac gacgttgtga acagcgcctg
    33061 cggcatcgga tgcgtttcca gccatttttc gaataactgg gcagccgcct cccgctcata
    33121 gctgttggca tacagaaaat gcacttcgcg cggatcatct ttccaggcag tacggaaacc
    33181 ttgttcacgc aggaagctga cagaaagctc cggcagcgcg ccaagataaa gcaccgtctc
    33241 ggcgggaaac ttacgtaact cttccgccag catttcggca tcatcctgat cggcaccaac
    33301 cacgctggta aagtgttcac gatcgagggc gcggtccagc gcgacaatcg ggaacgggtc
    33361 gttagcccag cgttgataaa aaggatgctc aggaggcaac gacgtcgaaa caataatggc
    33421 atcgacctga cgctgtaaaa ggtgctcaat acagcgcatt tcgttgtctg gctgatcttc
    33481 tgagcaggca atcagcagtt gatacccccg ttgccgcgcc tggcgttcaa gatagttagc
    33541 gatgcgggta tagctggtgt tctccagatc ggggatcaca agaccaatag aacgtgtgcg
    33601 tccagcacga agcccagctg ccacggcgtt cgggtggtaa ttgtgctcac gcacgacagc
    33661 catgactttt tcaacggttt tgtcgctcac acggtattgc ttcgctttgc cgttaataac
    33721 atagcttgca gtggtccgcg acactcccgc cagccgagcg atttcatcca gtttcacaat
    33781 tgccccttgc gtaaaatgta aaaaccatag ccattgtact ggtatgggtt aaattcatta
    33841 acatctaagc gcagaaaatt aactgcggca actgctttta ttccaggttc ccactgattt
    33901 cgcaaaaaaa gcccaacgtg acatgttggg ctgtaaattg cgcattgaga tcattctcaa
    33961 cgcattattt tatcgccgcg cgaaagtccg accacaccag agcgagcaac ttccacaatt
    34021 ttcgccacat cgcgaatcga tgctaaaaat gcatcaagct tatcgctggt gcctgctaat
    34081 tgaacggtat aaagcgaggg tgtgacatcg ataatttgcc cacggaatat ttccgtatta
    34141 cgtttcactt cgtcacgccc gtaaccgctg gcctgaattt tcaccagcat gatttccctc
    34201 tcaacatgcg cgccctgccc caactcactc acgcgcaaga catcgaccag cttgtgtaat
    34261 tgcttttcga tctgctcaag tactttttca tcgcccacgg tctggatggt catacgcgat
    34321 aatgtcggat cgtcggttgg cgcaacggtc aggctttcaa tgttgtagcc acgctgggaa
    34381 aaaaggccaa tcacgcggga taacgcgcct gattcatttt cgagtaagac tgataatatc
    34441 cggcgcataa tcaggttctc tccgttttgc ttaaccacat ttcatccatt ccgcccccgc
    34501 gaatctgcat cgggtagacg tgctcgctgc catcgacggt aacatcaaca aacaccaggc
    34561 gattactgcg cacctgttcc agcgcctcgc taagtttgct ttccagctca tgcggatgag
    34621 aaatctggat cccgacatgc ccataggctt ccgccagacg gacgaaatcg ggtagcgatt
    34681 gcatataaga ttgtgaatga cggccggaat agatcatgtc ctgccactgc ttcaccatcc
    34741 ccagatagcg gttattgaga ttcaccacca gtacgggcaa ctcgtattgc aacgcggtag
    34801 acagttcctg aatgttcatc tgaatactgc cgtcgccagt gacgcaaacc acggtttctt
    34861 ctggcaacgc cattttgacg cccagtgccg caggtaaacc aaaacccatc gtgccgaggc
    34921 caccggaatt gatccagcga cgcggtttgt cgaatggata ataaagtgca gcaaacatct
    34981 ggtgctgccc gacatcggac gtcacgtaag cgtctccctt cgtcaaccgc caaagagtct
    35041 cgatcaccgc ctgcggttta atcttttcac tgtgagtgtc atatttcagg cactgacgag
    35101 cgcgccactg ttcaatttgc tgccaccagt cgcggatctc atccagtggt tgatgggcgg
    35161 attcttgcga caagagttca agcatttgtt cgaggacctg gcgagcatcc cccacaatcg
    35221 ggatatccgc agtcacggtt ttagaaatgg aagtaggatc aatatcgata tgcagaacag
    35281 tggcatttgg gcagtacttt gccagattgt tcgtcgttcg gtcatcaaat cgtaccccga
    35341 cggcgaaaat cacatccgcg ttatgcatcg tcatattggc ttcgtaggta ccgtgcattc
    35401 ccagcatgcc cagtgcctga cgatgcgttg ccggaaacgc ccccagcccc atcaatgagg
    35461 aaacaacggg cagattcaac gcctccaccg tttctttcaa ctgctgatgg cagcccgccg
    35521 tgattgcccc accgcctacg tagacaaccg gtttttttgc cgctaccagc gtttgcagag
    35581 cacgcttaat ttgcccttta tgtccggtag tagtgggatt gtaagaacgc atactgaccg
    35641 actccggcca gacatagggt aatttgttcg ccggattaag aatatctttc ggtaaatcaa
    35701 cgaccactgg tcctgggcga ccacttgccg ccagccagaa agcctttttc agcacctgcg
    35761 gaatgtcttc cgtttgctta accagaaaac tgtgtttaac caccggtcgc gaaatcccca
    35821 ccatgtcgca ctcctgaaag gcatcgtaac ctatcaacga ggtcgctacc tgcccggaaa
    35881 ggacaactaa tggaatggaa tccatataag cggtggcgat gccagtaatc gcattggtcg
    35941 cccctggacc cgacgttacc agcacgacgc cgacttcccc ggtcgcgcgc gccagaccat
    36001 cggccatatg cactgccgcc tgctcatgac gaactaaaac atgatcgata ccacccacgg
    36061 tatgcaatgc atcgtaaata tcaaggactg cgcctccggg ataaccgaat acttgtttaa
    36121 cgccctgatc gataagcgat cggacgacca tctcggctcc agacaacatc tccatggcct
    36181 gcctcactgt ttgacggaaa aaatgtgtaa aacacattaa ccgtccgatc ttgcttaagc
    36241 aattggcagc cagagacata aacgccgggt aataagaata aacaggagga aaggtgaaaa
    36301 gaaacgataa aatcctccat tacgaaggat aa
//
LOCUS       DAZGBC010000030        35918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGBC010000030 DAZGBC010000000
VERSION     DAZGBC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.030"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(78..809)
                     /gene="rlmB"
                     /locus_tag="ACHK1J_001534"
     CDS             complement(78..809)
                     /gene="rlmB"
                     /locus_tag="ACHK1J_001534"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO7910831.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(990..3431)
                     /gene="rnr"
                     /locus_tag="ACHK1J_001535"
     CDS             complement(990..3431)
                     /gene="rnr"
                     /locus_tag="ACHK1J_001535"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO7910832.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3470..3895)
                     /gene="nsrR"
                     /locus_tag="ACHK1J_001536"
     CDS             complement(3470..3895)
                     /gene="nsrR"
                     /locus_tag="ACHK1J_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO7910833.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4100..5398)
                     /gene="purA"
                     /locus_tag="ACHK1J_001537"
     CDS             complement(4100..5398)
                     /gene="purA"
                     /locus_tag="ACHK1J_001537"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO7910834.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKERSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5502..5699)
                     /gene="yjeT"
                     /locus_tag="ACHK1J_001538"
     CDS             complement(5502..5699)
                     /gene="yjeT"
                     /locus_tag="ACHK1J_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO7910835.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5781..6785)
                     /gene="hflC"
                     /locus_tag="ACHK1J_001539"
     CDS             complement(5781..6785)
                     /gene="hflC"
                     /locus_tag="ACHK1J_001539"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO7910836.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6788..8047)
                     /gene="hflK"
                     /locus_tag="ACHK1J_001540"
     CDS             complement(6788..8047)
                     /gene="hflK"
                     /locus_tag="ACHK1J_001540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO7910837.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8133..9413)
                     /gene="hflX"
                     /locus_tag="ACHK1J_001541"
     CDS             complement(8133..9413)
                     /gene="hflX"
                     /locus_tag="ACHK1J_001541"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO7910838.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9490..9798)
                     /gene="hfq"
                     /locus_tag="ACHK1J_001542"
     CDS             complement(9490..9798)
                     /gene="hfq"
                     /locus_tag="ACHK1J_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO7910839.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9884..10834)
                     /gene="miaA"
                     /locus_tag="ACHK1J_001543"
     CDS             complement(9884..10834)
                     /gene="miaA"
                     /locus_tag="ACHK1J_001543"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO7910840.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10827..12674)
                     /gene="mutL"
                     /locus_tag="ACHK1J_001544"
     CDS             complement(10827..12674)
                     /gene="mutL"
                     /locus_tag="ACHK1J_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO7910841.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPTPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPHEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12684..14021)
                     /gene="amiB"
                     /locus_tag="ACHK1J_001545"
     CDS             complement(12684..14021)
                     /gene="amiB"
                     /locus_tag="ACHK1J_001545"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO7910842.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDLTLPN"
     gene            complement(14040..14501)
                     /gene="tsaE"
                     /locus_tag="ACHK1J_001546"
     CDS             complement(14040..14501)
                     /gene="tsaE"
                     /locus_tag="ACHK1J_001546"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO7910843.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14473..16020)
                     /gene="nnr"
                     /locus_tag="ACHK1J_001547"
     CDS             complement(14473..16020)
                     /gene="nnr"
                     /locus_tag="ACHK1J_001547"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO7910844.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQDSDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIASLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTEKNHD
                     ESSNSAP"
     gene            16019..17158
                     /gene="queG"
                     /locus_tag="ACHK1J_001548"
     CDS             16019..17158
                     /gene="queG"
                     /locus_tag="ACHK1J_001548"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO7910845.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17141..17194)
                     /locus_tag="ACHK1J_001549"
     CDS             complement(17141..17194)
                     /locus_tag="ACHK1J_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910846.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17428..17503)
                     /locus_tag="ACHK1J_001550"
     tRNA            complement(17428..17503)
                     /locus_tag="ACHK1J_001550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17468..17470),aa:Gly,seq:gcc)
     gene            complement(17539..17614)
                     /locus_tag="ACHK1J_001551"
     tRNA            complement(17539..17614)
                     /locus_tag="ACHK1J_001551"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17579..17581),aa:Gly,seq:gcc)
     gene            complement(17651..17726)
                     /locus_tag="ACHK1J_001552"
     tRNA            complement(17651..17726)
                     /locus_tag="ACHK1J_001552"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17691..17693),aa:Gly,seq:gcc)
     gene            complement(17937..18482)
                     /gene="orn"
                     /locus_tag="ACHK1J_001553"
     CDS             complement(17937..18482)
                     /gene="orn"
                     /locus_tag="ACHK1J_001553"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO7910847.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18577..19629
                     /gene="rsgA"
                     /locus_tag="ACHK1J_001554"
     CDS             18577..19629
                     /gene="rsgA"
                     /locus_tag="ACHK1J_001554"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO7910848.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19726..20694
                     /gene="asd"
                     /locus_tag="ACHK1J_001555"
     CDS             19726..20694
                     /gene="asd"
                     /locus_tag="ACHK1J_001555"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO7910849.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20716..24039
                     /gene="mscM"
                     /locus_tag="ACHK1J_001556"
     CDS             20716..24039
                     /gene="mscM"
                     /locus_tag="ACHK1J_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO7910850.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAGLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24190..25692)
                     /gene="yjeM"
                     /locus_tag="ACHK1J_001557"
     CDS             complement(24190..25692)
                     /gene="yjeM"
                     /locus_tag="ACHK1J_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO7910851.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25911..26888)
                     /gene="epmA"
                     /locus_tag="ACHK1J_001558"
     CDS             complement(25911..26888)
                     /gene="epmA"
                     /locus_tag="ACHK1J_001558"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO7910852.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27213..29021
                     /gene="frdA"
                     /locus_tag="ACHK1J_001559"
     CDS             27213..29021
                     /gene="frdA"
                     /locus_tag="ACHK1J_001559"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO7910853.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29014..29748
                     /gene="frdB"
                     /locus_tag="ACHK1J_001560"
     CDS             29014..29748
                     /gene="frdB"
                     /locus_tag="ACHK1J_001560"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO7910854.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29759..30154
                     /gene="frdC"
                     /locus_tag="ACHK1J_001561"
     CDS             29759..30154
                     /gene="frdC"
                     /locus_tag="ACHK1J_001561"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO7910855.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30165..30524
                     /gene="frdD"
                     /locus_tag="ACHK1J_001562"
     CDS             30165..30524
                     /gene="frdD"
                     /locus_tag="ACHK1J_001562"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO7910856.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            30587..31720
                     /gene="ampC"
                     /locus_tag="ACHK1J_001563"
     CDS             30587..31720
                     /gene="ampC"
                     /locus_tag="ACHK1J_001563"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7910857.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31809..32342
                     /gene="blc"
                     /locus_tag="ACHK1J_001564"
     CDS             31809..32342
                     /gene="blc"
                     /locus_tag="ACHK1J_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7910858.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32339..32656)
                     /gene="sugE"
                     /locus_tag="ACHK1J_001565"
     CDS             complement(32339..32656)
                     /gene="sugE"
                     /locus_tag="ACHK1J_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7910859.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32832..32978)
                     /gene="ecnB"
                     /locus_tag="ACHK1J_001566"
     CDS             complement(32832..32978)
                     /gene="ecnB"
                     /locus_tag="ACHK1J_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7910860.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33089..33214)
                     /gene="ecnA"
                     /locus_tag="ACHK1J_001567"
     CDS             complement(33089..33214)
                     /gene="ecnA"
                     /locus_tag="ACHK1J_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7910861.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33266..33832)
                     /gene="efp"
                     /locus_tag="ACHK1J_001568"
     CDS             complement(33266..33832)
                     /gene="efp"
                     /locus_tag="ACHK1J_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7910862.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33874..34902
                     /gene="epmB"
                     /locus_tag="ACHK1J_001569"
     CDS             33874..34902
                     /gene="epmB"
                     /locus_tag="ACHK1J_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7910863.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            35297..>35918
                     /gene="yjeJ"
                     /locus_tag="ACHK1J_001570"
     CDS             35297..>35918
                     /gene="yjeJ"
                     /locus_tag="ACHK1J_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7910864.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESG"
BASE COUNT         8582 a         9779 c         9130 g         8427 t
ORIGIN      
        1 cagtacgtaa ttatcttacc agctatagac aaaaaaaaac catccaaatc tggatggctt
       61 ttcataattc agaggaacta gctgcgctga cgcaccgctt caaataagca aattccggtt
      121 gcaaccgaaa cgttcaggga agaaacactt cctgccattg ggatgctgat caactcatcg
      181 caatgttcac gggtcaggcg acgcatacct tcaccttccg cgcccatcac cagcgccagg
      241 cgtccggtca ttttgctctg atagagtgta tgatccgcct cgcctgccgt accgacgatc
      301 cagatattct cttcctgcaa catacgcatg gtgcgcgcaa ggttagtcac ccgaatcagt
      361 ggaacgcttt ctgccgcgcc gcaggctact tttttcgccg tggcgttgag ctgtgcggag
      421 cgatctttcg gcacaatcac cgcgtgaacg ccagcggcgt ccgcgctacg caggcacgcg
      481 ccgaggttgt gcggatcggt tacaccgtcg aggatcagca ggaacggttg atcgagcgaa
      541 gcgatcagat ccggcagatc gttttcctga tactgacgtc ctggcttcac gcgggcgata
      601 atgccctgat gcacggcacc gtcgcttttc tcgtcgagat attggcggtt tgccaactgg
      661 ataaccacgc cctgagactc aagggcgtga atcagcggta acagacgttt atcttcacgg
      721 ccttttaaaa taaagacttc ctgaaaacgt tcaggggcgc gctccagcag ggcctgcact
      781 gcgtggatgc cgtaaatcat ttcgctcatt aatgtactcg ttgtttacgt tttgcctgat
      841 gcactacgtt tatccggcct acatgatctc tgcaatatat tgaatttgcg tgattatgta
      901 ggccggataa ggcgttcacg ccgcatccgg catgaacaaa gcgcattttg tcagcaatct
      961 aaccctcttc tgtttgaaga ggatattgat cactctgcca cttttttctt cgccgcacgc
     1021 ttcgctttgg tcgctgcggc tattttctgc gttttcgccg atggcttttt cgcttttctc
     1081 gcgtctttct tcgctgcttt cggcttcgtt tttttctcac cgcggaaggc gctgtctggc
     1141 tcaaagttta cctttttacc gacctgacga cgcttgccgc cttttttgcc tgcatcgcct
     1201 tttttcgctt tctcacgcgc cgttttaccg acgttgcgcg gtgcgcgttc gctggagatc
     1261 aggctaaagt cgattttgcg ctcgtccata ttaaccgctt cgacgcgaac ttccacgcga
     1321 tcgcccaggc gataagtctg gccgctggat tcacccatca ggcgttgccc tacctggtca
     1381 aagcgatagt agtcattgtc cagcgaagag acatggacca gcccatcaat gaacaagtcg
     1441 tccagacgga cgaagaagcc aaagccagtg acgctggaaa ttacgccttt aaagacgtta
     1501 cctacctggt cgagcatgaa gtcacacttc agccagtcag ccacatcgcg cgttgcttcg
     1561 tcggcacgac gttccgccat cgaacagtgc tgacccagtt gcagcatctc ttccatcgaa
     1621 taatggtagc caccggtttc agtggtgttg ccctgatgcc cctgctcttt cgccaacaga
     1681 tacttaatgg cgcggtgcag cgtcaggtca ggataacgac gaatcggcga agtaaagtgc
     1741 gcataggact gcaatgccag gccaaagtga ccacggtttt ccggatcgta aatcgcctgt
     1801 ttcatcgagc gcagcagcat ggtttgcagc atttctgcat caggacggtc ggcaaccgac
     1861 tccagcagct ccgcgtagtc gcgcggttcc ggcttgttac cgcccggcag ttccagcccc
     1921 agctctgcca gcactgaacg gaaagaggta atcgcttcgg tgctcggctt gtcgtgaata
     1981 cggaacagtg ccggttcttt cgctttctca acgaaacgcg ccgccgagat attcgccaga
     2041 atcatgcact cttcaattaa cttgtgtgcg tcgttacgct gagtctgttc gatacgttca
     2101 atacggcgtt cagcgttgaa aatgaacttc gcttcttcgc tctcaaatga gatcccgccg
     2161 cgttcttcac gggctttatc cagcacttta tagaggttat gcaactcttc gagatgctta
     2221 accagcgggg cgtactgctc gcgcagatcc tgatcgccct gcagaatatg ccagactttg
     2281 gtgtaggtca gacgcgcgtg agagctcatc accgcttcgt agaatttgta gccagtcagg
     2341 cggcctttcg acgaaaccgt catctcgcac accatacaca ggcggtctac ctgcggattg
     2401 agcgagcaca gaccgttaga gagcacttcc ggcagcatcg ggataacctg cgaagggaag
     2461 tacaccgacg tgccacggtt acgcgcttct ctgtccagcg gcgttggcgg acgcacatag
     2521 tagctgacgt cggcaatcgc gacccataaa cgccagccac caccgcgttt tttctcgcag
     2581 taaaccgcat cgtcaaagtc acgggcgtct tcgccatcaa tggtaaccag cggtaaatcg
     2641 cgcagatcga cacggcccgc ttttgcttct tccggcactt cttctttcag cccggcaacc
     2701 tgttgctcaa cagcctgcgg ccagatgtac ggaatttcat gggtacgcag agcgatatca
     2761 accgccatgc cggtgcccat attgtcgccc agcacttcga cgattttacc caccgctttg
     2821 gtacggcgag tcgggcgttg ggtcagttcg actacgacca caaagcccat ccgcgcgccc
     2881 ataatctgat cgggcgggat taaaatatcg aagctcagac ggctatcgtc aggaaccaca
     2941 aagccgacgc ccgcttcggt aaagtagcga ccaacaatct ggctggtttt tggcaccagt
     3001 acgcggacaa tacgcgcttc acgacgacct ttacggtcag cgcccagcgg ctgcgccagc
     3061 acctgatcgc catgaatgca ggttttcatc tgctcgctgg agagatacaa atcatcttta
     3121 cgcccttcaa cccgcagaaa gccgtagcca tcacggtggc caataacggt acctttcacc
     3181 aggtcgaggc gttccggcag cgcatagcac tgacgacgag tgaagaccag ttgaccatcg
     3241 cgctccatcg cgcgcaggcg gcgacgcagg ccttcaagct gctcttcgcc ttcaatgtgc
     3301 agttctaccg ccagctcatc acggctggcc ggtttttcac gtttggttaa atgttcgagg
     3361 ataaattccc gactagggat gggattcgcg tatttttcag cttcgcgttc ctggaaagga
     3421 tcttgtgaca tctcggttcc tccgttgtca tctctgatga agattttcgt cactccacca
     3481 gcaataattt ataaagcggt tgattctctt caaccaaatc ggcaagcgtg tagttatcca
     3541 gttccgtaag aaaactttgc acggccttag aaagcgcctg tttcaaccga caggcaggtg
     3601 taatgtggca aaactcactg ctgcaattca ccagcgataa gggctccagc tcgcgcacca
     3661 catcaccaat acgtatcgca ctcgccggtt tacccaagcg aatgccgcca ttttttccac
     3721 gaacagcagt cacgtagccg gcacgactaa gttgattgat tattttgacc atatgattac
     3781 gggagacgcc gtagacgtca gtcacttcag aaatactggt catccgccct tctggcaatg
     3841 acgccatgta gatcagcgca cgtaatccgt aatcagtgaa actcgttaac tgcacatcaa
     3901 cctcaaaagg gaaatcggga aaaaatacat ttatattgat gataaaccag ccacaagctg
     3961 tgtcgctaat ttatttcagt tcggggaagg aaaaaagcga ggatttaaca ctgtgtcgga
     4021 tagcgtaatg gcttaaccga cacagcaatg tatcacactg ttagcccagc aggcaaaata
     4081 tctgccaggc gtaccaaaat tacgcgtcga acgggtcgcg cagaatcatg gtttcagtac
     4141 gatccggacc ggtagagatg atatcgatcg gcacaccggt cagctcttca atacgtttga
     4201 tgtagttcag cgccgcctgc ggcagaccgc tacgttcttt cacgccgaag gtggattcag
     4261 accagcccgg catggtttcg taaatcggct ctacaccttt ccagtcgtca gctgccagcg
     4321 gagtggtagt cacttcgcga ccatccggca tacggtaagc cacacagagt ttcacctctt
     4381 tcaggccatc cagaacgtcc agtttagtca ggcagaagcc agacagggag ttcagctgta
     4441 ccgcacgacg aacggcaacg gtgtccagcc agccggtacg acgacgacga cccgtagttg
     4501 cgccgaattc gttaccctgc ttgcagagga actcgccagt ttcatcaaac agttcagtcg
     4561 ggaacggacc tgcacccaca cgagtggagt aagctttgag gatacccaga acgtaatcaa
     4621 cataacgcgg gcccaggccg gaaccggtcg ccacgccacc agcagtggtg ttggaagaag
     4681 ttacgtacgg ataagtaccg tggtcgatat ccagcagcgt accctgcgca ccttcaaaca
     4741 tgacgaaatc gccacgctga cgcgcctggt cgagcaggtc agaaacgtca accaccatag
     4801 aagtcaggat gtcggcaaca gccatcgtat catccagaac tttctggtaa tcaaccgctt
     4861 cagctttgta gtagttaacc aactggaagt tgtgatattc catcacttct ttcagttttt
     4921 cagcgaaggt ttctttgtcg aaaaggtcgc caacacgcag accgcgacgt gccactttat
     4981 cttcataagc aggcccgata ccacgaccgg tggtgccgat cgctttcgcg ccacgcgctt
     5041 tctcacgcgc gttatcaagc gcaacgtgat aatcaaggat cagcggacat gcttcagaca
     5101 gcagcagacg ctcacgaacg gggatgccac ggtcttccag ttctttcatc tctttcatca
     5161 gcgcggccgg agacagcaca acaccgttac cgatgatgct ggttacattc tcgcggagaa
     5221 tacctgatgg aataagatgg agaacggttt tttcaccgtt gattacgaga gtatggcctg
     5281 cgttgtgacc gccctggtag cgtacaacat atttagcccg ttcagtcaga agatcgacga
     5341 tcttaccttt accttcgtca ccccattggg tgcccagtac gacgacgttg ttacccattt
     5401 ttcaaaatca ccgtttgctt aaaaatggat tctaccatcg ctttttcaga gttacagcac
     5461 tttttgcact caaaaatgac ctcgatcagt ctgttttttg ttcagccaat cgttttcctc
     5521 aacatgtagt agaccacaac gcccgcaacc acaagtccac cgccaaaacg acgtaaaata
     5581 ttatcgggca aattggtcat cgcagagatc atcttcttcc atgccttcgg gtaaagcatc
     5641 ggccctaaac cttccagtac caaaaccagg gcaagcgcca gccagattgt cgaattcatt
     5701 cattatcctt atagaaaaag aaaaccaccg acatcccaga ggatgcggtg gctttattga
     5761 cctgtaccgc agtcgttata ttaacgcgtt gcggaagtcg gcgtcttcat gtagcggaag
     5821 aaatcgctat ccgggctcat gaccatcacg tcctgattgc cagagaagct gttctcataa
     5881 gcacgcaggc tacggatgaa tgcgtagaag tccggatctt tactgaatgc atcagcaaac
     5941 agtttggctg cttcggcatc gccttcacca cgcatgatac ggccctgacg ctcagcttct
     6001 gccagcgttc tggtcacttc atagtcggca gtcgcgcgca gtttttccgc ttcttcctga
     6061 ccttgtgaac ggtgacgacg cgctaccgct tcacgctcgg cgcgcatacg gttgtagatc
     6121 gcttcagaca cttcggtcgg caggttgatc tgcttgatac gcacatcgac aacttcaata
     6181 cccagcgccg ccatactgtt cgggttgatg accggaactt tgcccttcgt ctcagccgtt
     6241 acgcgctctg ccgcttcggc aatggcgtta tctgccgccg gggtagtaac ttcatcttct
     6301 gtacccgcag aaccggagtt cagcgcgtca cgtacttcga gggtcagacg accacgggaa
     6361 tcagtgacga tatctttcac gtccaggcga ccaatttcag aacgcagacg gtcagagaac
     6421 ttacgtttca acagcacttc cgcttgcgaa atgtcgccac cacccgttgc caggtagtaa
     6481 cggctgaaat cgctgatgcg ccatttgatg taagagtcga cgatcaggtc tttcttctct
     6541 ttggtcacaa agcggtcggc ctggttgtcc atggtctgaa tacgtgcgtc gagcattttc
     6601 accgtttcaa tgaacggtat cttgaaatgc agacccggct cataaaccag aggtttgttg
     6661 tcatcgtcac gcagtacctt accaaaacgc agcgtaatac cgcgctcacc ttctttgacg
     6721 acaaagacag acatgtaaag cactaccagc acgatgatga taatcgcgat aactgactta
     6781 cgcatcgtta ttccccctga cgctggtagt cgttacgctg cgcgttggcg cggcgttggt
     6841 ccataatatc gccctgactg gtggacgacg tgttgcttgc tccactggtt gtggaggaag
     6901 aggctggcgg cagacgcagc agattgctgg caccgttatc gctcttcgcc gcaggggcgt
     6961 taccaccttt cagcatctgg tctaacggca gaaccatcag gttgccacct ttatcgttaa
     7021 ccagcacttt gcgggtgtta cccaacactt tttccatcgt ctcgatatac agacgctcgc
     7081 gagtaatttc cggcgcggct ttatattccg gcagaagttt agcaaagcgc gccacttcac
     7141 cctgagcttc caggatggtc tgggccttgt acgcacgcgc ctcttcgagg atacgttgcg
     7201 cctgaccgtt cgcacgcggc tgaacttcgt tggtatacgc ttctgcttca cgaatgtatt
     7261 gctgttcgtt ttcacgcgcg gcaatcgcat cgtcaaacgc cgcttttact tcttccggcg
     7321 gacgagcagc ctggaagttg acgtccagca gcgtgatacc catgtcatac gggcggatag
     7381 tctcttccag ttcgcgctga gtatcgctac gaatcacggt acgaccttcc gtcagaatgc
     7441 ggtccatggt gtatttaccg ataactccac gcagggcgct gtcggtagcc tgacgcaggc
     7501 tgtcatccgg gctggtcacg ctatacagat atttttccgg attggtgacg cggtactgca
     7561 cgttcatctc aacgcgcact acgttctcgt ccgacgtcag catcacacca gaagcggcca
     7621 gttcacgcac ggcttccacg ttcaccggtt tgacttcgtc gataaacgtc ggtttccagt
     7681 tcagacccgg ctcaaccaga tggctgaatt taccaaagcg tgttaccacg ccacgttcgg
     7741 cttctttaat ggtatagaaa ccactggccg cccagataat gacaatcgct gccgctgcga
     7801 tggtaacgac acgaccgcca agctgcgggc gcgggccttg cgatgaactg ccaccgccag
     7861 atccggtgcc tttaccgccg cccagaccac cgagcttttt actcagtttg cggaagatat
     7921 catctaaatc aggtggccct tgatcgcgac cgcctttgtt tccatttccc tcagagttgc
     7981 cgccaggttt gctgcttccc cacgggtcgc ggtcttgtcc gttattaccg ggctgattcc
     8041 acgccatgtt tgtgctccat atttgttatg cggtgatccc tgttgtcttt cgacgccaga
     8101 ggatatgact ccacgcttca gacgctacgc cgttagatca ggtaatcgat caacgccggt
     8161 tcttgtttac agaggcgacg ccagtcaacg atcggcatac gaacttgcag acttacgctg
     8221 ccgtcctcct ccatccactc tttttctatt gcctgaagct gataaaaacg acttctcaga
     8281 cgcccttcct gcggtggcag acgcaatgta tgctgcgcca cctcgccgga aagccgctcc
     8341 gtcaaagcct gaaaaagctg tggtatcccc gctccggtct gtgcggaaag ccagacacgg
     8401 atcggtttgt tctcttcgtc ccgatcaata cgcggttcga aatcttccag catatcgatc
     8461 ttgttcatca ccagcagggt tgggatctcg tgagcgtcga tctcttcaag aaccgtattc
     8521 accgcttcga tgttttcttg tacacgcaca tccgccgcat caatgacgtg cagcagtaat
     8581 gtggcttgcc gcgtctcttg taacgtggct ttaaatgctg ccaccagatc gtgcggcagg
     8641 tggcgaataa accctacggt atctgcaagt acggtttcac cgacatctgc aacgtcaata
     8701 cgccgcaacg tcgggtcgag ggtggcaaac aactggtctg ccgcgtagac ccgcgcttcg
     8761 gtgatgcgat tgaaaagggt agatttaccg gcgttggtat atcccaccag cgaaacagta
     8821 ggaacgtcgg ctttgatacg cgattgccgc ccctgctcac gctgcttttc aactctttcc
     8881 aggcgcgact gtatctgcac gatgcgatta cgcaacaaac gacggtcggt ttcgagctgg
     8941 gtttcacccg gaccacgcaa acctatcccg cctttctgtc tttcaaggtg ggtccagcca
     9001 cgcaccaggc gcgtagccag atggcgcagc tgcgccagct caacctgcaa cttaccctca
     9061 tgggtacgcg cacgttgggc gaaaatatct aaaataaggc cggtgcggtc gataacacga
     9121 cactcgcaca aacgctccag gttacgctct tgcgccgggc tcagggcatg gtcaaaaaga
     9181 acgaccgaag cacccgtcgc tttgacagct tccgcaattt caactgcttt accttcacct
     9241 acaaaatact ttgggtgcgg cgctttacgg ctaccggtaa tcacctgcaa tgcttcgaca
     9301 ccggcggaag agaccagaga ttcaaactcc tggaggtctt ccatatcttt gtcttgcgta
     9361 aaatagatgt gtaccagtac cgcctgctca ccagcatcat aacggtcaaa caagcgtata
     9421 accctctaaa tagatcagcg gggaacgcag gatcgctggc tccccgtgta aaaaaaacag
     9481 cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg gaagtattct gcgcgctgct
     9541 accatgatgg tagttactgc tggtaccgcc accggcattg ttactgtgat gagaaaccgg
     9601 gcgagacggg acaacagtag aaatcgcgtg cttgtaaacc atctggctga ccgtgttttt
     9661 caacaggatc acgaactgat caaaagactc gatttgccct tgcagcttaa taccattcac
     9721 caaataaata gaaactggaa cacgttcccg acgcagtgcg ttcaggaacg gatcttgtaa
     9781 agattgcccc ttagccattc tctcttttcc ttatatgctt atttgtactt tgaacctttc
     9841 gattctgaaa aaattgcgca cgatacgtct caattgtaca cattcagcct gcgatagcac
     9901 caacaacctg taatacttcg tcacgcgcct gttctggttt ttcactatca agccagtgaa
     9961 ccccttccca accacgcagc caggttatct gccgcttcgc caactgtctc gtggcgcaaa
    10021 cacctcgata aaccatttca tcgtatgaga tttcgccttc aaggtaagac cacatctggc
    10081 gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa atctcctcgg gcaaaaagcg
    10141 cccggacttc tgcttcaaaa cctgaagcca acatctgatg aaaacgctgc tcaatgcgtt
    10201 gatggagcag ttcacggctc gccggggcga tggcgaactg atgcacctga tacggtagag
    10261 cgtctcctga cgtttgcgtc agttccgtta aagttttacc cgaaatgaaa aaaacttcca
    10321 gtgcccggga aagcctttgt ggatcatttg gatgaatcct tgctgccgca accggatcga
    10381 tctcctgaag ttgacgatgc aatgactccc aaccttgctc tgccgcctgt tgctcaattc
    10441 tggcccgtac ttccgggtct gccgacggta gcggcgacaa cccttccagc aatgccttga
    10501 aatacaacat cgtaccgccc actaacagtg ggatccgccc cgccgcggtg atatcggcca
    10561 tttccgccag cgcatcgcgg cgaaaatcag cagccgagta agcctgcgac ggatcgcgaa
    10621 tatccagcaa tcggtgcggc gcggcgagta actcttcagc gttcggcttc gccgtcccga
    10681 tatccatccc tttgtaaata agggcagaat caacgcttat caactctact ggtaaaattt
    10741 tacgcagctc aatggctaac gccgttttac cggaggccgt cggccccatc aaaaaaatcg
    10801 ccttaggcag gctcgcctta ctgatatcac tcatctttca gggcttttat cgccggatgt
    10861 aaatcaacag attgtaacag accacccggc ggcgttttca caagttgcgg acataaccgt
    10921 tccacgtccg ccagcagggt tatggcctgt gccattgacc actgcgcatg ttcgctcatc
    10981 agatttcgtg caatccactg cgcaatattg ccaggttcga atacggactg cttcgccagg
    11041 tagcctatca gttcaggaat caagatttgt aaattttgtt ggcgtaaggg taaaggcacg
    11101 gccctgatgg tcacatgctg tgcatctgac tggaaatcaa tacccaattc cgccagggca
    11161 gactgcgctt tttctaatgc cgatttttct tcgccagaaa cttttagccg caacggaatc
    11221 agcaacggct gggcgcaaac gggcgcttca cccggcgtca gttgtacctg acgcagccaa
    11281 cgttctgcca ctggcaaggc taaaagtgaa atgttgccgt cgcgctccag caacgcacag
    11341 tcggaatgga cgatagtcag tacccgacca aaactctgac tgttcgccgc aagtgcaggt
    11401 tcatgcggtt ctggcgcttt tggtttttgc atcggcgcgg gcgtttgcaa aagctggcga
    11461 tacacttcac cttgctgttt ctggtaacct ggttgcgcat tcggccaggg ggcagccgga
    11521 cgactgcctg atgctggcgc aggagtgtag cgcggagcta ctggctcacg aactgccggt
    11581 tcagcaaagt gattgcgccc cgccgccacg cggttttccg ggatggcacg cggtgtgggt
    11641 tgtggttcat cgtccagcgg tagcggcgtt tccagctgct gttgtagcac gctcagcacg
    11701 ccctgataga taaagtcatg caccaggcgc gactgatgga aacgcacttc gtgtttggcg
    11761 gggtgtacgt tgacgtccac ctgatgcggg tcgatctcca gatacaacac aaacgccggt
    11821 tgttgatcgg cccccagttt gtcttcgcag gcctggcgga tcgcgtgatt gatcaggcga
    11881 tcgcgcatca tgcgaccatt cacgtagcaa tactgaattt ccgccagtgc gggcgtggtg
    11941 tgatttggat cggccaccca gccgcgcagc gtgagatcgc cgtgttgcca ttcaatcgcc
    12001 agcgcttgtt cgagaaaagc ggtgccacaa atcgcgccta agcgccgttc tttttgcccg
    12061 ccttccggca ctgcgcggta ctggcgtacg attttgccgt tatgcgacag gttgatcgtg
    12121 acgtcgaaac gcgccagtgc gatgcggcgg atgatttcat cgatatggtt aaattcggtt
    12181 ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt agaacaggtc cagtacctcc
    12241 agcgtcgttc ccaccggatg cgccgccggt ttaaccgtca cgtccatatc gcgcccttcg
    12301 gcataggcct gccaggcttc ctgctgttct gcggtgcgtg aagtgagcgt caggcgggaa
    12361 accgaactga tactcgccag cgcctcaccg cgaaagccca ggctgataat ggcttcgaga
    12421 tcgtccagag aggcgatttt actggtggca tgacgcgcca gcgccagcgc cagctcgtct
    12481 tttttgatac cgcagccgtt atcacgaatg cggataagtt tcgccccacc gcgttcgata
    12541 tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt ccaccagttc tttgactacc
    12601 gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt tcgccagttg tggcggtaag
    12661 acctgaattg gcatcaatcg tccttagttt ggcagcgtaa gatctggcgt cgtcaccgta
    12721 ctggcagttt gtgccgttgc accctgcggc gcagattgca tcggatgcgc aaggaaataa
    12781 ttgcgcagac ctttataaat ggcttctgcc agctgttgtt ggtaatcgtc gctcgccagc
    12841 aaacgttctt cgctgttgtt gctgataaaa ccggtttcga ccagtactga tgggatatcc
    12901 ggcgaacgca gaacgccaag gctggcgtgt tctggtcgac gtttatgaat ttcgccaatg
    12961 cgttgcaact gactgatcat actggtcgct acatcatacc ctacccgctg ggaatgaccg
    13021 aactgtaaat ccagcacagc ctggcttaaa taggggtcag actgactgtt cgccagcaca
    13081 tcgcccgctc cgcccagtag ctccgactgt ttctcgtgct gctccagcca gctggccatt
    13141 tcgctgttgg cgcgacggtt agagagcacc catacggaag cgccagtcgc actgcggttc
    13201 ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt tttgcttacg tgccacatcg
    13261 ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg ttaaaacgcc cttaaacatc
    13321 ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg cgatggtgac atttttctcc
    13381 cgcgtaccgc cggggccgat agcgccaggg tcctgaccgc cgtgtccggc atcaatagcg
    13441 atgataattt tatcgccagt gttagccgtc gcgcgcgctg ccggacgcgt taccgtatta
    13501 ctgctgataa cacccgtagt gcggttactt tccgttttaa acggattgcg cgccggttcg
    13561 ctgacgcgcg gtgcgacaac cgtaggcgtt tcaacgcgtt tcgcaaccac aggcggcgga
    13621 ggaggcggtg gcggcgcatc ggcgttaatc gtaaagacga cagtgtaatt gctgccattc
    13681 tgccgcttca ccgcttcggt tttaccattt tcggtaagat cgaccaccag ccgtagcgtt
    13741 tgtgcatctt taggcgttcc ggagcgaatc gccttcacca gattattacc gctgaacaac
    13801 aacggcagtc cctgaatcac gcccgtttgt ttgatatcga gcgccacggt gcgtttgctt
    13861 tgatggctaa acgcataatc aggatcgcca ataaaactca acgttatccg cgcctgttga
    13921 ttaccgttag aaacctgaat atcagagagc gtcgcggcac ccacctgcgc gcacagcagc
    13981 agcaacgtcg ctaccaacca atttctgatg cgatacatca tcccgccacc tttcaaaggt
    14041 taaccggcta aacgcgccag caacaattca cccgcagagg aaaccgcact cacgcgcgcc
    14101 tcacggcctt gtgcctgata atcaatgtgt atttcgacat ccgggtcagg aagaacacct
    14161 gtaccttgtt gtggccactc caccaggcag atggcatcgt tggcaaaata gtcgcggatc
    14221 cccataaact ccagctcctc gggatcggca aggcggtaca aatcaaagtg ataaaccatt
    14281 aagttgtcga gcgtataggg ttcgaccagc gtataggtcg ggcttttgac attaccctga
    14341 tgacccaaag cctgcaaaaa gccccggcta aaggtggttt tacctgcgcc taaatcgcca
    14401 tacagataga tgacggttgc accatcgcag gctttggcta cccgctcgcc caggtctaag
    14461 gttgcctgct catcagggag cggaattact cgattcatca tggttttttt cagtcacttc
    14521 cgggttaaca atacgctgta gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc
    14581 aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg acacagcctg cacaggctgc
    14641 atcatacggc gacagttttt gcccaagcaa tgcgccaata ataccagaga gcacatcgcc
    14701 catgccgccg cttgccatgc ctgcatttcc ggcatcaata atgcctaaag cgtcaggatg
    14761 ggcggcgacc acggttccgg cacctttcag caccgctacg ccgccataac gttgtaccag
    14821 acgtttggcg caatgtaagc ggtcactttc aatttcagcg acggaacagc ctaacaaccg
    14881 tgcggcctcg ccaggatgcg gcgtgatcac gcgattgtga cgcttatcgg gattgattgc
    14941 cagcaggttc aatgcatcgg catcccacaa catcggtttg cgaaaattct caactttttg
    15001 cagggctttt ttcccccact cttgctggcc cagaccggga ccaatcacca ccacatcggc
    15061 ccattccagg ctttcggtaa gagagtccat agtcagttca tgcaccatca attctggtcg
    15121 tgcagtcagc agcgacgcaa tgttctcact gcgggtcagt actcggacta aaccagcacc
    15181 agcacgcagc gccgcttccc ccgtcatacg aatagccccc gccgtgccgt gatcgccacc
    15241 gataatcacc agccgcccgt gatcgccttt atgcgaagtc gggcgacgcg gtttcagcca
    15301 gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc tcctgacctg ccagccaact
    15361 atccaacccc agtgagtcaa aatgcagttg tccggtaaca tcccgcgctt ttccagtgag
    15421 caagcctggt ttcagcgcaa taaaagtgat ggtgtgatcg gcgttgatca ccgcacctgg
    15481 cgtagcgccg gtttcagcca gcaggccgga agggatatca accgccacaa tcggcgcagg
    15541 atgggaatta gcgtggtcga ttaactggct aatggattcg cggggcgctt gctgcaagcc
    15601 ggtaccgagc agcgcatcaa caatcagatc taccgattcg ggccagacaa tattcgaagc
    15661 atggatctcg ccacccgcgt ttaaccatgc ttcgcgtgcc agcgcggcct cttccggcaa
    15721 cggtttgtcg ctatcctggg ccaacaacgt gacctcaatg ccgaccgctt tggccagtcg
    15781 cgcgaccacg tagccatcgc cgccgttatt accatgaccg cacagcacca gccagtggcg
    15841 ggcgtcagga tacgccgaac gacacacctg gaatgcggcc tcgccagcgc gaagcatcag
    15901 ctcatagagt gtgagcccca gcacatctgc cgcctcgcgt tctccgcggc ggatatcgtc
    15961 ggcgtaccag acggtgtgtg gtatacttac ggggtttttc ttcattgtat ggtccgtcat
    16021 gtcagagccc ctcgatctca atcagttagc gcaaaaaatt aaacagtggg ggctggaact
    16081 gggctttcag caggtaggta ttaccgatac cgatctcagc gagtccgagc ccaaactgca
    16141 agcatggctg gacaaacaat accacggcga aatggactgg atggcacgtc acggtatgct
    16201 gcgcgctcgc ccccatgagt tattgcccgg tacgctgcgc gtgatcagcg tgcggatgaa
    16261 ttaccttcct gctaacgccg catttgccag cacgctgaaa aaccccaaac tcggctatgt
    16321 tagccgttat gcgctgggcc gtgactatca caaacttctg cgcaaccgac tcaaaaagct
    16381 gggcgagatg attcagcaac attgtgtttc gctgaatttt agaccgtttg tcgattctgc
    16441 gcctattctc gagcgcccgt tagctgaaaa agctgggctc ggctggacag gtaagcactc
    16501 acttatcctc aatcgcgagg ccggttcgtt cttcttttta ggcgaattgc tggtcgatat
    16561 tccgctgccc gtggatcaac cagtcgagga aggatgcggc aaatgcgtgg cctgtatgac
    16621 gatttgcccg accggtgcca tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc
    16681 ttatctcacc atcgaactgg aaggggcgat cccggaagag ttgcgaccgt taatgggaaa
    16741 ccgtatttac ggttgcgatg actgccagct tatctgcccg tggaatcgct attcacaact
    16801 caccacagaa gaggatttca gcccgcgtaa gccgctacac gcaccggaac tcattgagtt
    16861 attcgcctgg agcgaagaga agtttttaaa agtcacggaa ggatcggcga ttcgtcgtat
    16921 tggtcacctg cgttggctgc gtaatatcgc cgtagcatta ggcaatgcgc cctgggatga
    16981 aacgattctg gcggcgctgg agagtcgcaa aggtgagcac ccacttcttg atgagcacat
    17041 agcgtgggcg attgcgcagc aaatcgagag acgaaatgcg tgcatagtcg aagtgcaatt
    17101 gccgaaaaaa cagcgtctgg ttcgggtaat tgaaaaaggg ttaccgcgtg acgcctgatt
    17161 catccacagc ttgtgaataa aaataaaaac gcattgcaat tcaagacgaa taaaatcgtc
    17221 aagtgatcga ctcgacaaaa cggaatctta atttttaact tatctatcaa taggttaaaa
    17281 aaatactatt caccgtgcga agtttctttg aaattaagat tacagcctga ggctgtggat
    17341 aactctgttt acaagagttt gtcagagaca acaaaaacca gcaataacgc atcgcgtcgc
    17401 tgtggatatt ttattgagag aagaatttgg agcgggaaac gagactcgaa ctcgcgaccc
    17461 cgaccttggc aaggtcgtgc tctaccaact gagctattcc cgctttggtg gtctgtgcct
    17521 tacagcactt tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg
    17581 caaggtcgtg ctctaccaac tgagctattc ccgcttgggt ggtctgtgct ttgcagcact
    17641 tttcaaattt tggagcggga aacgagactc gaactcgcga ccccgacctt ggcaaggtcg
    17701 tgctctacca actgagctat tcccgcgtac tacttaattt tgctttcgta attttcgcat
    17761 ttctgcgtcg ttacgggagg cgcattatac gagaaatctt tccctctgca acccccctaa
    17821 aaaatttttt ttgagttttt atctcaagtg ctgaaattat cagcaaggcg ttcaatttag
    17881 cgtcaaaatc acgccgccgc aagtggattc cgacgcttac gtgaccagga aaaattttac
    17941 agcttgataa aatgctcgcg gtagtaagcc agctccgcca ccgattcacg gatatcatcc
    18001 atcgcctgat gcgtcccctg cttggtaaaa ccatccagaa tttccggctt ccagcggcgc
    18061 gccagctctt tcagggtgct gacatcgaga taacggtagt ggaagtaggc ttccagctcc
    18121 ggcatgtatt taaacaggaa gcgacgatcc tggccgatgc tgttaccgca aatcggcgat
    18181 tttcccgcag gcacccactg ttttaagaat tcgagcgttg ccagttcagc ttcacgatcg
    18241 cccatcgtgc tcgctttcac gcgctctacc agcccgctgg cggtatgggt gcgcacgttc
    18301 cagtcatcca tcagcgccag ctgttcatcg gactggtgta ctgcaatggt cggcccttct
    18361 gccagaatat tcaggttggc atcggtcacc agcgtggcaa tctcaataat gcgatcgcgc
    18421 tcgggatcca gaccggtcat ctcaagatcg atccaaatca ggttgttttc attggcactc
    18481 atgctatttt ccacccattc acagtgacta tattgatcca caattgcgtg tatcatagat
    18541 gttttgccca tcaggggcga ccaggagtct gtacgattga gtaaaaataa actctccaaa
    18601 ggccagcagc gccgcgtgaa cgccaatcac cagcgtcgtc ttaaaacgtc taaggagaag
    18661 cccgactacg acgacaatct gtttggcgag cctgatgaag gtatcgtcat cagccgcttt
    18721 ggtatgcacg ctgatgtgga atccgccgat ggcgacgttc accgctgcaa tattcgccgt
    18781 accatccgtt cgctggtgac cggcgaccgc gtagtctggc gtccgggtaa accggcggcg
    18841 gaaggcgtaa atgtcaaagg gatcgtggaa gcggtgcatg agcgcacctc ggtgttgacg
    18901 cgtccggatt tctacgacgg cgtgaaacct attgccgcca acatcgacca gattgtcatt
    18961 gtctccgcca ttttgccgga gctgtcactc aatattatcg accgttacct ggtggcctgc
    19021 gaaaccttgc agattgagcc gattattgtg ctcaacaaga tagacctgct ggacgacgaa
    19081 ggcatggcgt tcgtcaacga acagatggat atctaccgca atatcggtta tcgcgtattg
    19141 atggtttcca gccatactca ggatgggcta aaaccgctgg aagaggcgtt gaccgggcgc
    19201 atcagcattt ttgccgggca gtctggcgtc ggcaaatcca gcctgctgaa cgcgttgctg
    19261 gggctgcaaa aagagatcct gaccaacgat gtctctgaca actcggggct tggtcagcac
    19321 accacgaccg ccgctcggct gtatcacttc ccgcacggcg gtgatgtgat tgattcccct
    19381 ggtgtgcgtg agttcggcct ctggcacctg gagccggaac aaatcactca gggctttgtc
    19441 gaattccatg actatttagg tctgtgtaaa tatcgcgatt gcaaacacga taccgatccg
    19501 ggctgcgcta tccgggaagc ggttgaggaa gggaaaatcg cggaaacccg tttcgaaaac
    19561 tatcaccgta ttctggaaag catggcgcag gtaaaaacgc gtaaaaactt ttctgatacg
    19621 gatgactgac aattaagcta accgtcgtta gaatcgtccc ctttttttca ggatccggca
    19681 tgtaggccgg atcaggaacg acaaaacaat ggcctggagg ctaccttgtt aaattcattt
    19741 aaactttcgc tacagtacat tctgccgaaa ctatggctta ctcgcctggc gggttggggc
    19801 gcaagcaagc gggcaggatg gctgacaaaa ctggttatcg atctgttcgt taaatactac
    19861 aaggtcgaca tgaaagaggc gcaaaagccg gacaccgcca gctaccgcac ctttaacgaa
    19921 ttctttgtcc gtccgctgcg tgacgaagta cgcccaatcg ataccgatcc gaatgtcctg
    19981 gtcatgcctg ccgatggcgt tatcagccag ttgggtaaaa tcgaagaaga taaaatcctg
    20041 caagccaaag gccacaacta cagcctcgaa gccctgctgg caggcaacta tctgatggcg
    20101 gacctgttcc gcaacggtac gtttgtgacc acttacctct ccccgcgtga ctaccaccgc
    20161 gtacacatgc cgtgcaacgg tattctgcgt gagatgatct acgtgccggg cgatctcttc
    20221 tccgttaacc atctcacggc tcagaacgtg ccgaatctgt ttgcccgtaa cgaacgcgtg
    20281 atctgcctgt tcgataccga atttggccca atggcgcaga ttctggtcgg tgcgacgatt
    20341 gtaggcagca ttgagactgt ctgggcgggc accattacgc cgccgcgcga aggcatcatc
    20401 aagcgctgga cctggcctgc cggggaaaac gacggttctg tggcgctgct gaaaggccag
    20461 gaaatgggtc gctttaaact tggctctacc gttatcaacc tgtttgcacc gggtaaagtg
    20521 aatctggttg agcaactgga aagcctgtct gtcacgaaaa ttggccagcc gctggcagta
    20581 tctaccgaaa cctttgttac gccagacgct gaaccagccc cacttcctgc tgaagagatc
    20641 gaagcagaac acgacgccag cccattggtt gacgataaaa aagaccaggt ctaatccatc
    20701 aaaggaaacg ctgacgtgcg cctgattatc acttttctga tggcctggtg cctcagttgg
    20761 ggggcgtacg ccgcgacggc ccccgatagc aaacaaatca cccaggaact ggagcaggca
    20821 aaagcggcga aacccgcgca gccagaagtc atagaggcac tccagtctgc cttaaatgcg
    20881 cttgaggaac gaaaaggttc ccttgagcgc atcaagcaat atcaagaagt tattgataat
    20941 tatccgaaac tctccgctac tctgcgcgca caattaaaca acatgcgtga cgagccgcgc
    21001 agcgtgtcgc cggggatgtc taccgacgcg ctgaatcagg aaattctcca ggtcagcagt
    21061 cagttgctgg ataaaagccg tcaggcccag caagagcagg agcgcgcccg cgagattgcc
    21121 gattcgctga atcaactgcc gcaacagcaa accgacgccc gccgtcagtt aaacgagatc
    21181 gagcgccgcc tgggaacgct taccggcaat actccgctca atcaggcaca aaatttcgcg
    21241 ttgcagtctg actctgcacg tcttaaggcg ctcgttgatg aactggagct ggcgcagctg
    21301 tctgccaata accgccagga attagcgcgc ttgcgctctg agctggctga aaaagagagc
    21361 cagcaactgg atgcgtattt gcaggccttg cgtaatcaat tgaacagcca acgccagctt
    21421 gaggcagagc gggcgctgga aagtaccgaa ttactagcag aaaacagtgc cgatttgccg
    21481 aaagatatcg tcgcgcaatt caaaattaac cgcgaactat cggcagcgct gaatcaacag
    21541 gcgcagcgga tggatctcgt tgcctcgcaa cagcgtcagg ctgccagcca gacgttacag
    21601 gtccggcagg cgttgaatac gctgcgtgaa cagtcgcaat ggctgggatc gtccaatctg
    21661 ctcggtgaag cgttgcgggc gcaggtggca cggctgccgg aaatgccgaa accacaacag
    21721 cttgataccg aaatggcgca gttgcgtgtg caacggttac gttatgagga tctgcttaat
    21781 aaacagccgc tgctacggca aattcatcag gccgacggtc agccgctgac cgccgagcaa
    21841 aaccgtattc tggaagcaca actacgcact cagcgtgagt tgctgaactc attgttgcag
    21901 ggtggcgaca cactactgct ggaactgacc aagctgaaag tctctaacgg gcaactggag
    21961 gatgcgctga aagaggtgaa cgaagcgacg caccgctatc tgttctggac ctctgacgtg
    22021 cgcccgatga ccatcgcctg gccgctggaa atcgcccagg atctgcgtcg tctcatttcg
    22081 ctggacacct tcagtcagtt gggcaaagcc agtgtgatga tgctgaccag caaagagacg
    22141 attttgccgc tgtttggcgc gttgattctg gtcggttgca gtatttactc gcgccgctat
    22201 tttacccgtt ttctcgaacg ttcggcggcg aaagtcggca aagtgactca ggatcacttc
    22261 tggctgacgt tacgcactct tttctggtcg attctcgtcg cgtcaccgct gccggtgctg
    22321 tggatgacgc tgggttacgg cttgcgcgag gcgtggcctt atccgctggc ggtcgcgatt
    22381 ggcgatggtg taacggccac cgtgccgctg ctgtgggtag tgatgatttg cgccaccttt
    22441 gcccgcccga acggcttgtt tatcgctcat tttggctggc cgcgcgaacg tgtttcccgt
    22501 gggatgcgct actacctgat gagcatcggg cttattgtgc cactgattat ggcgctgatg
    22561 atgttcgata acctcgacga ccgcgaattt tccggttcgc tgggacggct ttgctttatc
    22621 ctcatttgcg gtgcgctggc ggtggtcacc ctcagcctga aaaaggccgg gataccgctg
    22681 tatctcaaca aagagggcag cggcgacaat attaccaacc atatgttgtg gaacatgatg
    22741 attggcgcgc cactggttgc cattctggcg tcggcggtgg gttatctggc aacggcacag
    22801 gcgctgttag cgaggcttga aacctcggtt gccatctggt tcctgctact ggtggtttat
    22861 catgttatcc gccgctggat gctgatccag cgccgcaggc tggcgtttga ccgggcgaag
    22921 catcgccggg cagagatgtt agcgcaacgt gcacgtggcg aagaggaagc gcatcatcac
    22981 agtagcccgg aaggagcaat tgaagtcgat gaaagcgaag tcgatctcga tgccattagt
    23041 gcgcaatcct tgcggctggt gcgctcaatt ttgatgttga tcgccctgct ttcggtcatt
    23101 gtgctgtggt cagaaatcca ttccgctttc ggcttcctcg aaaatatttc gctgtgggat
    23161 gtcacttcca cggtacaggg cgtggaaagt ctggagccga ttaccctcgg tgcggggctg
    23221 attgccattc tggtgtttat catcaccacg cagctggtgc gcaatctgcc cgcgctgctg
    23281 gaactggcga ttttgcagca cctggattta acgccgggta ccggctacgc catcaccacc
    23341 atcaccaaat atctgctgat gctgattggc gggctggtcg gcttctcaat gattggtatt
    23401 gagtggtcga aattgcagtg gctggtcgcc gcgctcggtg ttggtctcgg ttttggtttg
    23461 caggaaattt tcgccaactt tatctctggc ctgatcatcc tgttcgaaaa accgattcgc
    23521 attggcgata cggtaacgat ccgcgatctt actggtagcg tgacaaaaat taacacccgc
    23581 gccaccacca tcagcgactg ggaccgtaaa gagataatcg tgccgaacaa ggcgtttatt
    23641 accgagcagt ttatcaactg gtcgctctct gactcggtca cgcgcgtggt gttgacgatt
    23701 ccggcccctg ccgatgccaa cagtgaagaa gtgacggaaa tcctgctcac cgcagcgcgt
    23761 cgctgctcgc tggtgatcga caacccggca ccggaagtct tcctggtgga tctgcaacag
    23821 gggattcaga ttttcgagct gcgtatttac gccgctgaga tgggtcaccg tatgccgcta
    23881 cgccatgaga tccaccagct gattctggct ggcttccatg cccacggtat cgatatgcca
    23941 ttcccgccct tccagatgcg tctggaaagc ctcaacggta aacaaacggg gagaacgctg
    24001 acgtctgcgg gcaaaggtcg tcaggcggga agtttgtaat gcatgaagct gccaaatgat
    24061 gtcaacgtgg ctcatttagc agcttaacat catcttcagt tatcaccggc cctggagagt
    24121 atcatccata cgcattactt cctgacaact catcgccgga gagacgatga gttgcaggat
    24181 ttcatatgat cagtcgagag ctaactcagg tttattcgcc atgcgactgc aatagttctg
    24241 gtaaatcgcc atcgctaaca gcgagaagaa gacagggccg ccaatcatcc acaatgtgct
    24301 gtcccagtct ccggcttcaa ccacaggttg aatgatggtg aagacgttcg caaatgtcac
    24361 caccagtacg acaaccactg tcgcaatcat tgccgacata cgcgttttga aaatcacaaa
    24421 tggtctgtcg agatcctgac gtgctttaaa gaatgggaaa gccagcgcga ggaacaggta
    24481 aggaagcgtc atagacacgt tcgccatcag cgtcagcttg ttaaagaacg ccgatgcggt
    24541 accgccacca aacgaaacca gcaggatgaa gacagtaacc aacccgcact gcatccacat
    24601 ggcgatagac ggcatcccca tcgcattcag gcgcgtcatc ggttccggcc acaacgcttt
    24661 cggcgtcccc tggatgatgg ctttcagcgg tgaatagcaa agcgtaaaga acgcaccggt
    24721 ataagcgagg aacatcgaaa gtccggtaat acgcgcaaac catacgccca gcgacaatga
    24781 cgcttcaggt gacaaatgca gtgcgttacc cagcgtcacc ccgaggctct tcatcagcac
    24841 ataggtaata ttgccaaggt taacggaacc attacttaat acctgctgcc agtttgtgct
    24901 gacgccccat aaaaatattg ccagcgaata accgattgaa ataacaatag cggcaaaaac
    24961 aatacctttg gcaaagttct tttctggatt ttccgtttta tcgaccagac caccaaccgc
    25021 ttcaattccg ccataggcaa aaatagcaaa taccacaaac gatagcattg ccagaccgga
    25081 ctgataacct gggttcggtg atgcaaggaa attaatatcc tgcgcgaaat gcccaccatt
    25141 taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca ttactgcaat
    25201 accccccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa cgacggtgac
    25261 cagaatcatc catgccactg ccagcagacc aaccacctgc gtaggctcca gtccggcaat
    25321 acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta cccaaacttt
    25381 cgcggaggtg ctcaccatcc agatgatata agaggaaaac cacataaacg taccaatgaa
    25441 ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccgcctt cttctttgcg
    25501 ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata acaatgcaga
    25561 aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg ggctattggc
    25621 aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca tcttttttat
    25681 cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taaaacgcgc ccactgaact
    25741 gcactgccca acattgagcg ggcagcgtaa aaaccaatac agaattgata cctggatgat
    25801 atcaaatatc cagtcttaaa ttgtggcctt tagcggaaaa atgaaaacat atgctacaga
    25861 atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa ttatgcccgg
    25921 tcaacgctaa aggcgatgac ttcagccagt gtctccgcac ccagcgccaa catcaccaga
    25981 cgatcaacgc ctaatgccac gccggaacag tcaggcatac cgactttcaa ggcttcaatc
    26041 agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt
    26101 tcaaagcgtt gttgctgctc acgggcatcc gtcaattcat ggaaaccatt cgccagctca
    26161 atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt actgatttgc
    26221 gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc tttaccaata
    26281 tttggttcta cgccaaaggt aaacagcaat tgtagcaacg tgtcgcggtc ttcttcggta
    26341 tcagcaacat tgctcaaatc cagtttcgct gccacttccc gcagttgcgt tttgtcggca
    26401 gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag gctttctgct
    26461 gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa ccggtacata
    26521 tcatagtgcg gtcgatacca ctcaagcata gtgaactcag ggttgtgata acgccccatc
    26581 tcttcattac ggaaactgcg gcacagctgg aataccggcc cgcaaccggc aaccagcagg
    26641 cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg cgaatgcccg
    26701 gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt cgcctggctc
    26761 atacaaggcg tctccacctc cagcactcca cgatcggcaa agaaacgacg gatctccgcc
    26821 ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca ggatgccgtt
    26881 tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg caacgcgacg
    26941 gcgagacaaa ttttacgcag gaataaaaca acggtgggca gtgactaaaa aaagcacgat
    27001 ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc ttgcgcacta
    27061 aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt gtacctataa
    27121 aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct gaagtacggg
    27181 gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca
    27241 ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa
    27301 atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc gttgctgcag
    27361 aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac tttcacgata
    27421 cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc gtccaccact
    27481 gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt cgcccggatg
    27541 gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgtacctgg ttcgccgccg
    27601 ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga
    27661 tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt catgttcgcg
    27721 gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct aacgcggtcg
    27781 ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta
    27841 ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac atggaattcg
    27901 ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa ggttgccgtg
    27961 gcgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat tacggcatgg
    28021 gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt ccacgcgaca
    28081 aagtctctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg
    28141 gcgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc
    28201 cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc
    28261 cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag aactgtgaaa
    28321 cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttgggctg cacggtgcaa
    28381 accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtctg gccggtgaac
    28441 aagcgacaga gcgtgcagca actgccggta atggcaacga agcggcaatt gaagcgcagg
    28501 cagctggcgt tgaacaacgt ctgaaagatc tggttaacca agatggcggc gaaaactggg
    28561 cgaagatccg cgacgaaatg ggcctggcta tggaagaagg ttgcggtatc taccgtacgc
    28621 cggaactgat gcagaaaacc atcgacaagc tggctgagct gcaggaacgc ttcaagcgcg
    28681 tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc attgaactgg
    28741 gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt aaagagtccc
    28801 gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca
    28861 aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac agcgacgtga
    28921 agattactac gctaccgcca gctaaacgcg tttacggtgg cgaagcggat gcagccgata
    28981 aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt
    29041 gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg
    29101 ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga caacctggca
    29161 ccggacctga gctaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg
    29221 gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg
    29281 aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac
    29341 ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat
    29401 cagggtacta acatccagac cccggcgcag atggcgaagt atcaccagtt ctccggttgc
    29461 atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc
    29521 ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag
    29581 aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc
    29641 tactgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca gcagggcaaa
    29701 gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat
    29761 gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc
    29821 gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat
    29881 tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg cgggattcgt
    29941 cgacttttta caaaacccgg ttattgtgat cattaacctg atcactctgg cggcagctct
    30001 gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca ttgtaaaaga
    30061 cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg tagttgccac
    30121 catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc
    30181 caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg
    30241 caattattgc gccggtgatg atcctgctgg ttggtattct gctgccgctg gggctgtttc
    30301 cgggtgatgc actgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg
    30361 tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg
    30421 cgatgcacga tctgaaaatc cacgtacctg cgggcaaatg ggttttctac ggtctggctg
    30481 ctatcctgac agttgtcacg ctgattggtg tcgttacaat ctaacgcatc gccaatgtaa
    30541 atccggcccg cctatggcgg gccgttttgt atggaaacca gaccctatgt tcaaaacgac
    30601 gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc aacaaatcaa
    30661 cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc cgggtatggc
    30721 ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg cggacatcgc
    30781 caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt
    30841 tacgggcgtg cttggtggcg acgctattgc tcgaggggaa atcaagttaa gcgatcccgc
    30901 aacaaaatac tggcctgaac ttaccgctaa acagtggaac gggatcacgc tattacatct
    30961 cgcaacctac accgctggcg gcctgccatt gcaggtgccg gatgaggtga aatcctcaag
    31021 cgacttgctg cgcttctatc aaaactggca gcctgcatgg gcgccaggaa cacaacgtct
    31081 gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt ctggtttgag
    31141 ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc aaactcaacc atacgtggat
    31201 taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag gtaaggcagt
    31261 gcatgtttcg ccaggggcgt tagatgctga aacttatggt gtgaagtcga ccattgaaga
    31321 tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat atcaacgaca aaacacttca
    31381 gcaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct
    31441 gggctgggaa atgctggact ggccggtaaa tcctgacagc atcattaacg gcagtgacaa
    31501 taaaattgca ctggcagcac gccccgtaaa agcgattacg cctccaactc ctgcagtacg
    31561 cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg tcgcgtttat
    31621 tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca atccagcgag
    31681 agtcgccgcc gcctggcaga ttctcaacgc tctgcagtaa aatttcatcg ggtccgaatt
    31741 ttcggacctt ttctccgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg
    31801 aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg
    31861 cctgcagttc tcctacgccg ccgcgtggcg tgaccgtagt gaataatttc gacgccaaac
    31921 gttatctcgg tacctggtat gagattgccc gttttgatca ccgctttgag cgtggactgg
    31981 aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc attaataaag
    32041 gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac tttaccggcg
    32101 caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg
    32161 ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac cgcgactacc
    32221 tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag
    32281 tcgcgacccg ggaagggttt gatgtcagta aatttatttg ggtacagcag cccggcagtt
    32341 agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga ctcgccaggc
    32401 gcatcgggtt agcggactca ccgagcagca caatgccggt gatggccgcg ccgactgcgc
    32461 caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc gcccaggcaa
    32521 gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc aaacgactaa
    32581 agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta
    32641 agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg aaaagacgcg
    32701 tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa
    32761 tttcaactat tacctttgtg actatcgcag agaaaacaaa cggcacgaca cagctgtcgt
    32821 accgttattg cttattgctg cgctttcgtt gctgcaccag aaatcgcgtt accgccatca
    32881 gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc
    32941 agaacagaaa agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg
    33001 ttatttatgt ttgcctatag caaattaata atagacaaca tcgccaaaaa tgatggaaat
    33061 ctgatgattt ttcggaagag aagaaaaatt agctggcagc gcgggagatg gagttgccga
    33121 gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca gcccgtgagc agcgtgctgg
    33181 caagcaaaac aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca
    33241 gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg
    33301 tatccacttt gatgacttcg ccgatttgta cgaacagcgg aactttaacc acagcgccag
    33361 tagacagggt agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg
    33421 tatcaacgat ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc
    33481 acagagttac gatacactct gcctgatcca gcagccattt cgcgttgtcg ccaattgctt
    33541 ttgcgtcagc agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt
    33601 cgttgtacag gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag
    33661 atttgaaggt tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa
    33721 atgcctggcc tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt
    33781 ctaacatgat tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct
    33841 ctgaaatttg ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc
    33901 ccatccagag aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt
    33961 ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag
    34021 ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac
    34081 gatccgcttt tgcgtcaggt aattacctcg caagatgagt ttgccatcgc gcccggattc
    34141 tccaccgacc cgctggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac
    34201 aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa attgccgtta ttgcttccgt
    34261 cgccacttcc cttatgcaga aaatcagggc aacaagcgta actggcaaac tgcacttgag
    34321 tatgttgctg cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg
    34381 gcgaaagatc acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa
    34441 cgtctgcgga ttcacagtcg tctgccgatt gtgatcccgg cacgcatcac agatgcgctg
    34501 gttgaacgct tttcacattc tacgctgcaa atcttgctgg tgaatcacat caaccatgcc
    34561 aatgagatag atgaaacatt ccgtcaggcg atggctaagt tgcgccgggt aggcgttact
    34621 ttgctgaacc agagcgttct gttacgtgat gtgaacgata acgcacaaac gctggcaaac
    34681 ctgagtaatg cgttgttcga tgccggcgtg atgccgtatt acctgcatgt gctcgataaa
    34741 gtacagggtg cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag
    34801 ttgctgacac tggtgtcggg atatctggtg ccgaaactgg cgcgagaaat tggcggcgaa
    34861 cccagcaaaa cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt
    34921 caaacattaa aattgcctga tgcgctacgc ttatcaggcc tacatcgtgc attgaaattg
    34981 aatggatttg caatgaactg taggccggat aaggcgttta cgccgcatcc ggcaagagca
    35041 acaaacgcaa gtacagcaac ctgtcattaa acagggaagt ttaatctttg cccgttgcat
    35101 aacctttaat tattcatatg cattgctgtt gttactcttt atataactaa caccaataat
    35161 taacctttct tttctgttta tatagagtga gtacggctat ttatgttatt ttaacaacta
    35221 ttactaccaa gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg
    35281 gttagggaga acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa
    35341 gagtaataat ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt
    35401 gttctttatg tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca
    35461 tcaaaagcat aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt
    35521 cattaagaaa atggcagaaa atatcccaga aattctggtt gatgagctta aaaatgccga
    35581 tattaatcgt cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt
    35641 cgtattaacg ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga
    35701 aatgctggcg cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt
    35761 acgtattacc tcactgttag atttcctgcc gttatacgac gttgactgcc agcaaaatgg
    35821 caatctggaa tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta
    35881 tctggcggtg ctttaccgct ttaaagatga aagtggca
//
LOCUS       DAZGBC010000031        35023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGBC010000031 DAZGBC010000000
VERSION     DAZGBC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.031"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..661
                     /locus_tag="ACHK1J_001571"
     CDS             65..661
                     /locus_tag="ACHK1J_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910865.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIEPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            748..1473
                     /locus_tag="ACHK1J_001572"
     CDS             748..1473
                     /locus_tag="ACHK1J_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO7910866.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFGVTGLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1515..4115
                     /locus_tag="ACHK1J_001573"
                     /pseudo
     CDS             1515..4115
                     /locus_tag="ACHK1J_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
     gene            4129..4677
                     /locus_tag="ACHK1J_001574"
     CDS             4129..4677
                     /locus_tag="ACHK1J_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910867.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVQD
                     GGPVVTGSNGEYSLTIPDVGLDKVVTGDVAAQANFKLVASDCSNGYSKIFTTLTGTTT
                     SGKLIVNEASSGATGVGMGIKRRDTADSTFFTPNNTDKFEWTADEKTNGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4692..5306
                     /locus_tag="ACHK1J_001575"
     CDS             4692..5306
                     /locus_tag="ACHK1J_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910868.1"
                     /translation="MKLKVIATLIATVAVGVSVSNNLTYASTTSASLTVNSNLTMGTC
                     SAKVVDTSGAVIDTVVLGNVYISELVAKSKVQPFKIRFSDCAGLPGKSAQLVLAPRAN
                     VGCAGGMSNTAAFSNQLNSTTDGGSSRAAVEVWTTTSPEGNGSEQFNCYQRNVINVDL
                     SNASTTQPYDFDLSARMIIAEGWSVNDVLPGRFLSPTTFTITYQ"
     gene            5327..5917
                     /locus_tag="ACHK1J_001576"
     CDS             5327..5917
                     /locus_tag="ACHK1J_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO7910869.1"
                     /translation="MLCRHHKFVRFLGLATALITPFAYAGQDVDLTAQIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQVNNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5929..7041
                     /locus_tag="ACHK1J_001577"
     CDS             5929..7041
                     /locus_tag="ACHK1J_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7910870.1"
                     /translation="MQRKGNKLLIQLCSAILLFFTTSWYALANECYIERNAEGPYDMV
                     INPKPFSIQSAMVTVPEEIGFATWDSYIDLRGDKIGCKSLGSNTSSVHFLNTADASLL
                     STYTTSHGDALLKTTVPGIVYSVELVCTSCGAAEELDLYLPAQSGADNHTDNASSKWA
                     YEYSDESWYLRFRFFWTPEFKPQNGVSEGTAIPGKIASWYIGNNDQPWINFFVDTSSV
                     HFYVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKI
                     TVKLKAANKPSDATLVGKTNGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAVWSN
                     NLLLLPFTAQLVPDGSGAAVGVGTFSGNATFSFTYE"
     gene            7177..7971
                     /gene="panB"
                     /locus_tag="ACHK1J_001578"
     CDS             7177..7971
                     /gene="panB"
                     /locus_tag="ACHK1J_001578"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO7910871.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALVIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7983..8834
                     /gene="panC"
                     /locus_tag="ACHK1J_001579"
     CDS             7983..8834
                     /gene="panC"
                     /locus_tag="ACHK1J_001579"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO7910872.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLQEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8930..9139)
                     /locus_tag="ACHK1J_001580"
     CDS             complement(8930..9139)
                     /locus_tag="ACHK1J_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910873.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYQEELQKVRQEFAQRFLSK
                     GMSREDVAEVTTLPLTEIDKLINSN"
     gene            complement(9208..10080)
                     /gene="rpnC"
                     /locus_tag="ACHK1J_001581"
     CDS             complement(9208..10080)
                     /gene="rpnC"
                     /locus_tag="ACHK1J_001581"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO7910874.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            10354..10734
                     /gene="panD"
                     /locus_tag="ACHK1J_001582"
     CDS             10354..10734
                     /gene="panD"
                     /locus_tag="ACHK1J_001582"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO7910875.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10738..11967)
                     /gene="yadE"
                     /locus_tag="ACHK1J_001583"
     CDS             complement(10738..11967)
                     /gene="yadE"
                     /locus_tag="ACHK1J_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7910876.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12031..12471)
                     /gene="yadI"
                     /locus_tag="ACHK1J_001584"
     CDS             complement(12031..12471)
                     /gene="yadI"
                     /locus_tag="ACHK1J_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7910877.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12576..13346)
                     /gene="yadH"
                     /locus_tag="ACHK1J_001585"
     CDS             complement(12576..13346)
                     /gene="yadH"
                     /locus_tag="ACHK1J_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7910878.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13343..14269)
                     /gene="yadG"
                     /locus_tag="ACHK1J_001586"
     CDS             complement(13343..14269)
                     /gene="yadG"
                     /locus_tag="ACHK1J_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7910879.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14378..15040
                     /gene="can"
                     /locus_tag="ACHK1J_001587"
     CDS             14378..15040
                     /gene="can"
                     /locus_tag="ACHK1J_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO7910880.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15081..15617)
                     /gene="hpt"
                     /locus_tag="ACHK1J_001588"
     CDS             complement(15081..15617)
                     /gene="hpt"
                     /locus_tag="ACHK1J_001588"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO7910881.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15823..18213
                     /gene="gcd"
                     /locus_tag="ACHK1J_001589"
     CDS             15823..18213
                     /gene="gcd"
                     /locus_tag="ACHK1J_001589"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO7910882.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRYSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            18252..18350
                     /locus_tag="ACHK1J_001590"
                     /pseudo
     CDS             18252..18350
                     /locus_tag="ACHK1J_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18415..19965)
                     /gene="cueO"
                     /locus_tag="ACHK1J_001591"
     CDS             complement(18415..19965)
                     /gene="cueO"
                     /locus_tag="ACHK1J_001591"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO7910883.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20131..20478
                     /gene="yacC"
                     /locus_tag="ACHK1J_001592"
     CDS             20131..20478
                     /gene="yacC"
                     /locus_tag="ACHK1J_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO7910884.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20584..21450
                     /gene="speE"
                     /locus_tag="ACHK1J_001593"
     CDS             20584..21450
                     /gene="speE"
                     /locus_tag="ACHK1J_001593"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO7910885.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21466..22260
                     /gene="speD"
                     /locus_tag="ACHK1J_001594"
     CDS             21466..22260
                     /gene="speD"
                     /locus_tag="ACHK1J_001594"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO7910886.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22298..22660)
                     /gene="yacL"
                     /locus_tag="ACHK1J_001595"
     CDS             complement(22298..22660)
                     /gene="yacL"
                     /locus_tag="ACHK1J_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO7910887.1"
                     /translation="MDYEFLRDTTGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22835..25432)
                     /gene="acnB"
                     /locus_tag="ACHK1J_001596"
     CDS             complement(22835..25432)
                     /gene="acnB"
                     /locus_tag="ACHK1J_001596"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO7910888.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEKEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25787..27634
                     /gene="yacH"
                     /locus_tag="ACHK1J_001597"
     CDS             25787..27634
                     /gene="yacH"
                     /locus_tag="ACHK1J_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO7910889.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAILAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHDDDYHHHDGGHRDGNGWQHNGD
                     NINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQ
                     LPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFSRR
                     QPLTQQQKDAARQRYQSASPEPRQAVRERMQTNPQNQQRREAARERIQPASPEQRQAV
                     REKMQTNPQIQQRREAARERIQSASPEQRQVFKEKVQQSPLNQQQRDNARQRVQSASP
                     EQRQVFREKVKESHPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27705..29129)
                     /gene="lpdA"
                     /locus_tag="ACHK1J_001598"
     CDS             complement(27705..29129)
                     /gene="lpdA"
                     /locus_tag="ACHK1J_001598"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO7910890.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29337..31229)
                     /gene="aceF"
                     /locus_tag="ACHK1J_001599"
     CDS             complement(29337..31229)
                     /gene="aceF"
                     /locus_tag="ACHK1J_001599"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO7910891.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31244..33907)
                     /gene="aceE"
                     /locus_tag="ACHK1J_001600"
     CDS             complement(31244..33907)
                     /gene="aceE"
                     /locus_tag="ACHK1J_001600"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO7910892.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34068..34832)
                     /gene="pdhR"
                     /locus_tag="ACHK1J_001601"
     CDS             complement(34068..34832)
                     /gene="pdhR"
                     /locus_tag="ACHK1J_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO7910893.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYARREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8593 a         9257 c         8593 g         8580 t
ORIGIN      
        1 aaaaactttg agtaatatcc gctcgcaata ttaatttcat ataatttttg taaggatgca
       61 tgtaatgaaa aaagcacttc tcgcagccgc tctggttatg gcttctggtt ccgccctggc
      121 tgtagatggt ggtcatatcg actttaacgg tatggtacag tccggtacct gtaaagtggg
      181 tgtggtagat actggtatgc acagcgttac cactgatggc gtggttaccc tggatactgc
      241 gaatgttact gatacttttg ctgaagttag cgcaactgct gtcggtttac tgccgaaaga
      301 gttcatgatt tctgttgagt gtgatccagg tgctccgaag aatgctgagt taactatggg
      361 ttctgcaagc tacgcgaaca ccagcggtac cctgaataac aatatgaaca tcactgttaa
      421 cggtattgaa ccggctcaga acgtaaacat tgcagttcat aacatgaaaa acaaagctgg
      481 cgctgctgaa attaagcagg tccatatgaa caactcttct gaagttcagg aactgacatt
      541 agacgcagaa ggtaaaggcc agtacgtatt taacgcatct tacgttaaag caccgaacag
      601 cccggctgta actgctggtc atgtaaccac taacgcgctg tacaccgttg cttataagta
      661 attgatggtg tgttgagcat aaggtttgcc agcaggcaaa ccttattttc gcgaatttaa
      721 tctttctgca gacagcaagg agtgcttatg tttcccttgg ttaaaaaaac ggttagtgca
      781 ttatttgtga gtactctgtt agccagtgca ccagcattcg ctgacattat tatttccgga
      841 acacgtatca tttataacgc tgataaaaaa gatgttaacg ttcgtctgga aaataaaggg
      901 aatcgcccct tactgattca gaactggttg gatactggcg acgacaatgc cgatcccagc
      961 cagatcaaag tgccgtttac cgcgacccca ccagtttcac gtgtggagcc aaaacgcggt
     1021 caaaccgtaa aagtgatgta taccggtgca acagccctgc cgaaagaccg tgaaagcgtc
     1081 tactggttta acgtgctgga agtgccgcct aagccgaaag atgctgaggc tgacaaaaac
     1141 ttgttacagc tggctttccg cacacgcatc aaactctttt atcgcccgag tgggttacag
     1201 ggagaaccgg ctgaagcacc ggcgaaaatc acctggaaat taaacaacgc tcagttacag
     1261 gcaaataacc caacgccata ttacgtgtcc tttaatgagg tcaagctgga aagcggcggc
     1321 aaaacctaca acgtcaattc cagcatggtc gcgccgttca gccaggcaag ctttggcgta
     1381 accggtctgc caggaagcgt gtcatccgga aaagtggttt ttaaagcgat caacgacttt
     1441 ggcggcaaca ttgatggttc cgcaacattc taaaaaataa acggattctt tttaacatcg
     1501 ccaggaatgc aaccttgtac cagtttactc atcaaaaaag ccgtatcccg aaaaaaacgc
     1561 tacttgcggc ctgttgcgcc cttttttaca gcggcaacag ttctgcggca gacacggtgg
     1621 aatatgacag ttccttctta atgggaactg gcgcatcaac gattgatgtt aaacgttatg
     1681 ctcaaggcaa cccgacaccg ccgggtctct ataatgtccg cgtgtttgta aacggtcagg
     1741 cgacgtccag cttagatatt ccgtttgtgg atattggcga aaacagcgca gcggcctgta
     1801 ttacccacaa aaacctggca caacttcaca ttaagcaacc tgaacagcct gtcactttac
     1861 tcgccagaga aggtgaggaa gaggattgtc tggatttggc aaagtcatac gaaaaggcgg
     1921 atgtgcgctt tgacggtagt gaccagtttc tcgatctgac gatccctcag gcctatgttt
     1981 tgaaaagcta tggcggctac gttgaccctt ctttatggga atcgggaatt aacgctgcca
     2041 cactggcata taccctgaac gcgtatcaca caagttcaga taacgacaat agtgacagcg
     2101 tctatggcgc atttaactcg ggtatcaatt taggagcctg gcacttccgt gcgcgcggta
     2161 actataactg gacaacagat aacggcagcg atttcgattt ccaggatcgt tacttacagc
     2221 gtgacattcc ggcaatccgt tcacagataa ttatgggtga cgcctacacc accggtgaaa
     2281 cgtttgactc tgtcaacgtc cgtggtgttc gcctgtacag cgatagccgt atgctacctt
     2341 cggcgctggc cagttacgcc ccgaccatcc gcggtgtagc aaactccaac gccaaagtta
     2401 cggtgacgca gaacggttat aaaatctatg aaactaccgt tccgcccggt gaatttgtta
     2461 tagacgacat tagcccctcc ggctttggta gcgaactggt cgtaaccatt gaagaagcgg
     2521 atggttccaa acgcaccttt acgcaaccct tctcgtcggt tgtgcaaatg caacgtcctg
     2581 gtgtaggtcg ttgggatttc agtgccggta aagtcattga tgatagcctg cgttccgaac
     2641 ccaatatggg gcaagcctct tattactatg gtctgaataa cctcttcacg ggttataccg
     2701 gcattcagtt caccgataac aactatcttg ccgggctgtt aggtgtgggt atcaacacca
     2761 gcatcggcgc ctttgcggta gacgttaccc attcccgtgc tgaaattccg gatgataaaa
     2821 cctaccaggg gtaaagttat cgcgtgacct ggaacaaact tttccaggat accgggacat
     2881 catttaacct cgcggcgtac cgctattcca cccaggatta cctgggcctg catgatgcgt
     2941 tagtcctcat tgacgacgcc aaacatttgt ctgccgatga agacaaaaac accatgcaga
     3001 cgtactcacg tatgaaaaac cagtttaccg tcagcatcaa ccagccgctg aaaacgactt
     3061 acgaagatta cggttcactg tttatttccg gtagctggac ggattactgg gcggcgaaca
     3121 atagccgcac tgaatataat gttggttaca gtaaaagcgt ttcgtggggc agtttcagcg
     3181 tcaacctgca acgtagctgg aatgaagacg gcgagaaaga tgacgcgatg tacgtcagcg
     3241 ttagcgtacc tattgagaat attttaggtg gcaagcgcaa atcttctggt ttccgcaatt
     3301 taaatactca gctcaatacc gatttcgatg gttcacatca gttgaatgtt aacagttccg
     3361 gtaacactga aaacaatctg gtgaactaca gtgtcaacgc tggttatagc ctcgataaaa
     3421 actccggcga tttagcctct gttggcggtt atctcaacta tgagtctggg ttaggcggta
     3481 tttccgcttc ggcctcggcc acttctgata atagccagca gtactccatc tccaccgatg
     3541 gcggctttgt attacacagt ggtggtttaa cgttcactaa caacagtttc agcagtaacg
     3601 atacgctggt gttaatcaac gccccaggtg ctaaaggcgc acgaatcaat aacagtaata
     3661 acgaaatcga tcgctgggga tatgccgtga cgtcctctgt cagcccctac cgtgaaaacc
     3721 gggtaggtct gaacattgaa acactggaaa acgatgttga actgaaaagt accagcgcaa
     3781 ccaccgtacc acgcagcggc tccgttgttt tgacccgttt cgaaactgac gaggggcgtt
     3841 ctgccgtgct gaatattact gccgccaatg gcaaatccat tccgtttgct gcggaggttt
     3901 accagggtga ggtgatgatc ggcagcatgg gccagggtgg tcaggcattt gtgcgcggta
     3961 ttaacgacag cggggaatta acagtgcgct ggtatgaaaa caaccaaacc gttgactgta
     4021 agttgcacta ccagttcccg gcgcagccac aaacgctggg aagtaccaac accttattac
     4081 ttaacaatct tacctgtcag gtagcaaatc actaatatgg aaaattctat gaaaaggata
     4141 ctactaacat ccgcgttaat aggtctgggt ttaccggcgg ttggctcagc aacagatctt
     4201 aatgtcgatt ttacggccac cgtattagcc accacctgta cgataactat cgtacaagat
     4261 ggtggtccag tagtgacggg tagcaacggt gaatatagcc tgacaattcc cgatgttggc
     4321 ctggataagg ttgtcactgg agatgttgca gctcaagcca actttaaatt agtggcttcc
     4381 gattgcagca atggttattc aaagattttt accacactta caggaacaac gacttccggt
     4441 aagttgattg tcaacgaagc aagctctggc gctacgggtg tgggtatggg gattaagcgt
     4501 agagataccg cagactcgac tttctttaca cccaataata ctgacaaatt tgaatggacc
     4561 gccgatgaga aaactaacgg tgtgcctctg accgtagcac tgcgcgaaac aacggcggga
     4621 gcaggacgca ccggtgcgtt tcaggcaaaa gccaccttta acttcaccta cgaataacag
     4681 ggacagccac catgaaatta aaagtcatcg ctacactgat tgctactgtt gccgtgggtg
     4741 tcagcgttag caacaatctc acttatgcga gtacgacatc cgcctcttta accgtaaaca
     4801 gtaacctgac tatgggtacc tgtagtgcga aggtagtgga taccagtggt gccgtgatcg
     4861 acaccgttgt cttgggcaat gtttatattt ctgaactggt agccaaaagc aaggtacaac
     4921 catttaaaat ccgcttcagc gattgtgccg gccttccagg aaagagtgct caactcgtct
     4981 tagcgcccag agcaaatgtc ggttgtgcag gcggcatgtc taacactgca gccttttcaa
     5041 atcaactcaa ctccaccacc gatggtggtt caagcagagc agcagtcgaa gtatggacga
     5101 ctacaagccc cgagggaaat ggcagtgagc aattcaattg ttatcagcga aacgtcatca
     5161 atgtcgatct ttctaatgcc agcacaacac agccttatga ttttgattta agcgcacgaa
     5221 tgattatcgc tgaaggatgg tcggtcaatg acgtattacc gggtagattc ctctctccta
     5281 cgactttcac aatcacttat cagtaatagc ggataaggat attagaatgc tatgcaggca
     5341 tcataaattt gtacgttttt tggggctggc aacagccctc attacgcctt ttgcctatgc
     5401 tggccaggat gtggatctca cggcgcagat tgtggccagc acctgtcagg ttgaagtcag
     5461 taataatggc gttgtcgatc tcggcacagt gacgctggac tattttgccg acaatgtaac
     5521 cccgacaacg gattatgcgg gtggcaaaac gtttaatgtg aatgtggtct cttgcgataa
     5581 cattcaaacg acacagtcgc aaatgaaatt ggattttcag ccgcaggcgg gatcgcttgc
     5641 acaggtgaat aatcaaattt ttagtaatga atacgaacag caggctaccg gggcgaaaaa
     5701 cgtcggcatt gtgatatttt cggcccaacc gaatcagcag acatttaacg tgcgaggaac
     5761 ggatggttcc tcgacagcaa tttattcggt cgcgcctggc aatgctgttc cctcaacctg
     5821 gaccttttat tcccgtatgc agcgggtgaa taatgccctt cctcctgaat cgggaatggt
     5881 gagaagccag gttattgtta acgtatctta cgaataataa ggataattat gcaaaggaaa
     5941 ggcaataaac tgttgattca gttatgcagt gcgatactgc tattttttac cacatcctgg
     6001 tatgcattgg cgaatgaatg ttatatagag agaaatgctg aagggcctta tgatatggtg
     6061 ataaacccaa agcccttcag tatacagtca gcgatggtca cagtcccgga agaaataggc
     6121 ttcgcgacct gggattctta tattgatctt cggggcgata agattggttg taaatcctta
     6181 ggtagtaaca caagctcagt gcattttctt aatactgctg atgccagtct cttatcaacc
     6241 tatactacca gccatggcga tgctttattg aaaacaaccg ttcccggtat tgtttattca
     6301 gtagaattag tatgtacaag ttgtggagcc gcggaggagc ttgatttgta tcttcctgca
     6361 caaagtggtg cagataatca tacagataac gccagctcaa agtgggccta tgagtatagt
     6421 gacgagagct ggtatttacg atttcgtttt ttctggaccc cggaatttaa acctcaaaat
     6481 ggggtttcag aaggaactgc gataccaggt aaaatagctt cctggtatat aggcaataat
     6541 gaccaaccat ggataaattt tttcgttgat acaagctccg ttcattttta cgttgacgaa
     6601 ccgacctgtg caacagttgc cctcgcacaa gatcagggca acgtcagtgg caaccaggta
     6661 acgcttggga acagctatgt ttcggaagtg aaaaatgggc ttacgcggga aatccctttt
     6721 tctatccgtg ctgaatactg ctatgccagt aaaattacgg ttaagttgaa agcggcaaat
     6781 aaacccagcg acgccacact agtaggtaaa acgaatggct cggcttcagg cgtggctgta
     6841 aaagtaaatt caacttatga caatagcaaa gtattgttaa aagcagatgg tagcaacacg
     6901 gttgactata acttcgccgt ctggtcaaac aacctgctgc ttttaccttt tacggcgcag
     6961 ctggtaccgg atggcagtgg tgctgctgtc ggtgttggaa ccttctctgg taacgcgacc
     7021 ttctccttta cctacgaata agtaaaacgt cattccttcc tcatcgcacc agagttctgc
     7081 tctggtgcga acgttttccc cacactaaaa accattgccc ccctgatttc gactgttaga
     7141 atgcgatcaa tctgacttta gccatcaggg tacgttatga aaccgaccac cattgcttca
     7201 ttgcaaaaat gcaaacagga aaaaaaacgt ttcgcgacca tcaccgctta cgactatagc
     7261 ttcgccaaac tctttgctga cgaagggctg aacgtgatgc tggtgggtga ttcgctgggc
     7321 atgaccgttc agggccacga ctccactctg ccagtaaccg ttgccgatat cgcctaccac
     7381 actgccgccg tacgtcgcgg cgcgccaaac tgcctgctgt tggctgacct gccgtttatg
     7441 gcgtatgcca cgccggaaca agcctttgaa aacgccgcaa cggttatgcg tgccggtgct
     7501 aacatggtca aaattgaagg cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa
     7561 cgtgccgttc ctgtatgtgg tcacttaggt ttaaccccac agtcagtgaa tattttcggt
     7621 ggctacaaag ttcaggggcg cggcgatgaa gcgagcgatc gactgcttag cgatgcatta
     7681 gccttagaag ccgctggtgc acagttgctg gtgctggaat gcgtgccggt tgaactggca
     7741 aaacgtatta ccgaagcact ggtgatcccg gttattggca ttggcgcagg caacgtcact
     7801 gacgggcaga tcctcgtgat gcacgatgcg ttcggcatta ccggcggcca tatccctaaa
     7861 tttgccaaaa atttcctcgc tgaaacgggc gacatccgcg cggctgtgcg gcagtatatg
     7921 gctgaagtgg agtccggcgt ttatccgggc gaagaacaca gtttccatta aggagtgacg
     7981 ttgtgttaat tatcgaaacc ctgccactgc tgcgccagca aattcgccgc ctgcgtatgg
     8041 aaggcaagcg cgtggcgctg gtacctacca tgggcaacct gcatgatggc cacatgaagc
     8101 tggtcgacga agccaaagcc cgcgccgatg tggtcgtcgt cagtattttc gttaacccga
     8161 tgcagttcga ccgcccggag gacctggctc gttacccacg caccttgcag gaagactgcg
     8221 aaaagctgaa caagcgcaaa gtggatctgg ttttcgcgcc ctcggtaaaa gagatctacc
     8281 cgaacggtac tgaaacccat acttacgttg acgttccagg gctttcgacc atgctggaag
     8341 gcgccagccg tccggggcat tttcgcggcg tgtcgactat cgtcagcaag ctgtttaacc
     8401 tggtccagcc ggacatcgcc tgcttcggtg aaaaggactt tcagcaactg gcgctgatcc
     8461 gcaaaatggt tgctgatatg ggctttgata ttgagattgt cggtgtgcca attatgcgcg
     8521 ccaaagacgg tctggcactg agttcccgta acggttatct gacggcagaa caacgcaaaa
     8581 ttgcgcccgg tctgtacaaa gttttaagtt cgattgccga caaattgcag gctggcgaac
     8641 gggatctcga tgaaattatt gctattgccg ggcaagaact gaatgaaaaa ggcttccgcg
     8701 ccgatgatat tcagattcgc gatgccgaca cgttgcagga ggtctctgaa aacagcaaac
     8761 gggccgtaat tctggtagcc gcctggcttg gcgatgctcg cctgatcgac aacaagatgg
     8821 tcgaactggc gtaatactta actggcgcta cggctgatgg cgccagttat taatttgccc
     8881 tacgtcatgg cgatgacgtc tgtaagtgat tattcatatc gctggcaatt tagttagagt
     8941 taattagctt atcaatctct gtaagaggta aagttgtcac ctctgcaacg tcttcccgag
     9001 acatcccttt acttagaaaa cgctgggcga attcctgtct taccttctgg agttcctctt
     9061 gatatccctt ctgaaatccc ttctcttcaa accactgcgc caacgtctgc ctggtttctc
     9121 tccgtgtctt tatttccata ggttccctta attatctggt cagttaacaa taacaattat
     9181 ctggctataa ctgatgatgc agacaactta gttaatcagc ttatcaatct cggcaagggg
     9241 taaatttgcc atctctgcaa cgtcttcccg agacattcct ttattcagaa gacgcaggac
     9301 gaattcttgc ctttcctcct gtcttcccct ctcttcaaac cactgcgcca gcgtcatcat
     9361 agactcccct cccgtttccc tgtctctcag cacaccataa aacaaatccg cttgttcagt
     9421 atgaccgcgt tgcagcatat agttttgcat ggcaactaac tgacttccgc tagtgtaccc
     9481 ttcgtcaatc agcgtgacca gttgctccag caataacatt aagtcgcgct ggcgaatatg
     9541 tttttgcagt agttcgagaa tcgcaatccg ccgatgttgc atgatttcgt catccggtgt
     9601 gatggtgata tccaccagcg ggaaaggact gttatagacg cgtcgcgcca gctcaggcga
     9661 gtaaaacata tcaaaccagc acattgagag cggataaggt gtggcctcgc cctgataaaa
     9721 cagtatcggc accaccagcg gcagcttatc gtggtcagcc tccagatgcc ggtgcatggc
     9781 ggcaatagaa taacgcatca tgcgaaaagc cattttctta tctggcttgc tttgatgttc
     9841 aatcactaca tgcagataac cgggattgcc ctgcatttgc acggaataga gcacgtccgt
     9901 gctgtgtcct ttcaggctct cttcaatgaa actccccgac tctaaatgaa gcgtgttgag
     9961 gtcacaaagt tctcgtaatt ccactggcaa atgtatctcc agaaagtcgc gagccgtctc
    10021 cgcatgcatt aaaaattgtt taaataccgc gtcatgcggt gtggtactcg gtgcatccat
    10081 cttccctgtc ttccgtgttg tcttgcgtga aggcagtatc cagtcaaggc gtgctatcgt
    10141 cagccacctc tttagtgttt accgagcagc gttcagagaa atttcaattg gttacagccg
    10201 ttacatggca cgcttcggcg cggtacgcaa actgctgatg caaatgccgt cagatgagta
    10261 gacactaaac aaaaatcggg caatactgcg tgagaatttc ctcaaagcag gcaccgcctg
    10321 cttcgttaac gacagggtag aaaggtagaa gttatgattc gcacgatgct gcagggcaaa
    10381 ctccaccgcg tgaaagtgac tcatgcggac ctgcactatg aaggttcttg cgccattgac
    10441 caggattttc ttgacgcagc cggtattctc gaaaacgaag ccattgatat ctggaatgtc
    10501 accaacggca agcgtttctc cacttatgcc atcgcggcag aacgcggttc gagaattatt
    10561 tctgttaacg gtgcggcggc ccactgcgcc agtgtcggcg atattgtcat catcgccagc
    10621 ttcgttacca tgccagatga agaagctcgc acctggcgac ccaacgtcgc ctattttgaa
    10681 ggcgacaatg aaatgaaacg taccgcgaaa gcgattccgg tacaggttgc ttgattgtta
    10741 tccctgcggc tggttactca ccagccgcga catcgtctcc agcgaatccg ttcttaagat
    10801 ataaagtcgt tttagtaaca acggattatc ccccggtttt actttgcctt tcatggttgt
    10861 caccgccagg tgaaatccgg catcgtttgc tgccttcacg gctttgtcat taaagccgcc
    10921 aaacggatac gaaagatacc agacatgcgg attaaattgc gccagagcgc ggcgtgaacg
    10981 tgcaaaatca aacagaatat tgtgctcact acggctcagt aatatgggtc ggcgataacc
    11041 atctacccga tgcaaaaaat gggtatgtga ctggaaatca aatacatcgc gaatttcgtt
    11101 aagctcagaa acgctcataa attgcagcga ttttgggttc cacttctgcg ggtgacgttt
    11161 gatgcgtgag gtaacaataa acgccgtcgc cttcatgcca tattgtttca acacaggata
    11221 cgcatagcgg ctcaccgact tgaggccatc atcaaaggta atcaccaccg ctcgcgcagg
    11281 gagattgatc ttattcttca cgtaaccttc cagctgcacc atgctcagtg tcgcgtatcc
    11341 cctgtcgcgc agccaggcca tctggttatt gaaagcgcgt accgaggtgg tcgtcgaagt
    11401 atggcgaaaa cgggtgtttt cttcgtcgcg cagaatatgg tgataagtta gcaccggaag
    11461 gccattatcg ggttgggcat ccagcgcgct gatataggcc agtcgatcgc caatacggac
    11521 ctgataccag gtttgattta acctgtcttt cagtttatgc aaaatcgggt agcgcaaatt
    11581 gtccgccagt accccaaatg gcgcactgcc cgcactcggc gcgttataga ccggcgtatc
    11641 tttccaggta actaagttct gattactcag cggcttgttg aggtcaccca aaccgtcttc
    11701 aactttttgt cgcccctgaa ccggctcgag atgcccttta tcgataaacc ctttaccaaa
    11761 gccaaaatta aatgcgtaat aacttgcggc agtgggctcc acggcaatga tttgtccggc
    11821 ccgaatattc cccacggtca ccatcttgtc accaatttgc gcccagaccg cagcattttc
    11881 gatggtttgc atataacggg caggtaacgc ggcactgaca cttgcggtaa acagcatcag
    11941 caggagaata acagcttgtt tgtacatgaa aaaataaccg agggtgaaac aacgctttta
    12001 ttgtagcaaa agcgctataa gaatcgtggg ttaatactca tacaaattat gtttgaggac
    12061 gaaaggcgga tttttttgtt gttggtgcca aagactactc acctccggcg cacccagttc
    12121 gacaatacgc tcgcgaaact ctgaactggt catggtttca cggcaagcca tcatctgttc
    12181 aattaacggt aacgtgacac cagaaatcac ttcgcaacgg gagtgtttgt ggcttaataa
    12241 tgaagccacg cgatacggtg gcgctccggc tatatcggtt aagaagatga caccctcacc
    12301 agagtccgct tcatgcagag catcgcacat catgcggctg agcatattag agcttaaccc
    12361 acgccagaaa ttcacggccc ggcactgaag aagcgcccca tgttttttct ccagcgcatc
    12421 cagtatctct tgcgcccggt catcgtgaca ggtaattacc caacctaaca tcgccttcct
    12481 ccttagccga aaatcagtct accctgacca ttgttgataa agggtgatag ccgtcaaaac
    12541 tcctctcccc caaatccaga ggagaggaaa tagccttagc tacgcaaacc acgtccacgt
    12601 tggatcagcg accaacagat caaataaaac gccacaataa agaccaccag tacgccaaag
    12661 gtagtgacca gcggaacatc attgataccg aggaagccgt agcggaaacc actgatcata
    12721 taaacgattg ggttcaggtg cgacagccct tgccagaacg gcggcaacaa agtcagggag
    12781 taaaagaccc cacccaaata cgtgagtggc gttaacacaa aggttggcac caggctgatg
    12841 tcatcgaacg ttttggcaaa cacaccgttc agcaaacccg caagggagaa caacaccgcc
    12901 gtgagcacca gggttaaggc aacgaatacc cacgaatgca cctgaaacgg cacaaaaaac
    12961 agtgaaattg ccgtcaccag aatgccaaca aacagaccac gcgccacgcc accgccgaca
    13021 tatccggcaa taatgacgtg agtcggaacc ggcgctacca gcagctcttc aatattacgc
    13081 tggaatttgg caccaaaaaa tgatgacgcg acgttggcgt aggcgttggt gatcaccgac
    13141 atcatgatca gccccggtac gatgaactgc atgtagctga agccatgcat atcgccaata
    13201 cgcgaaccaa tcaggttacc gaaaataata aagtaaaggg tcatggtgat aactggcggc
    13261 accagcgtct gcacccagat acgcataaag cgatggatct ctttcgccca gatgcttttt
    13321 aacgccaccc agtaaagatg catcatgcgc gatctccttg tttttcatta accagtgaaa
    13381 caaacagctc ttccagacgg ttagctttgt tacgcatact taatacctga atgccctgct
    13441 cacttaactg cgtaaatacg ctgttgatcc cctgctcacg cagcacttca acttccagcg
    13501 tcgcggtatc gaccagtcga tactgatagc catcgagctt cggtaacgga cttttcggtg
    13561 cgagatcgag aataaaggtt tccgatttca gcttcgccag cagcgccttc atcgaggtat
    13621 tttccaccag ctcaccgtgt tgaataatgc cgatattgcg gcacagcatt tctgcttctt
    13681 ccaggtagtg tgtggtgaga atgatggtgg tgcctttgtc gtttaaatcc ttcaaaaagc
    13741 cccacattga gcggcgaagt tcaatatcca cgcctgcagt cggctcgtcg agaatcagta
    13801 gtttaggttc atgcattaac gcacgggcaa tcattaaacg gcgcttcatc ccgccagata
    13861 acatgcgcgc acgttcgttg cgttttcccc ataaatcgag ctgcttaaga tatttttcgc
    13921 tgcggatgta cgcctctttg cgctccacac cgtagtaccc tgcctgattc accacaattt
    13981 gctgcacggt ttcaaacggg ttgaagttaa attcctgcgg caccagtccc aactgacgtt
    14041 tagcgttcac gacatctttc tcgagatcgt aaccaaatac gctgacccgc ccggaggttt
    14101 tatttaccag agagctgata ataccgatag tggtcgattt cccggccccg ttcggcccga
    14161 gaagcgcata aaaatcaccc gcttcgacct gcaaatctat tccacgaagc gcctgaacgc
    14221 cgcctggata ggttttttta agctgttgaa gttccagtgc aatggtcata aatttttact
    14281 taccttacgt tcttacactt tatctgtggt ttaaatcgtc ccggagttgc cctatattag
    14341 ccaaacgtaa ttatttggtt acaggtcgtt aacctccatg aaagacatag atacactcat
    14401 cagcaacaat gcactatggt caaaaatgct ggtggaagag gatcccgggt tttttgagaa
    14461 actggcacaa gcgcaaaaac cgcgctttct atggattgga tgttccgaca gtcgcgttcc
    14521 tgcagaacgt ttaaccggtc ttgagccggg cgaactcttt gttcaccgta atgttgctaa
    14581 cctggtcatt cacaccgacc tgaactgcct ttccgtggtt cagtatgcag tggatgtact
    14641 cgaagttgaa cacattatta tctgtggcca ctacggttgc ggcggcgtac aagccgcagt
    14701 tgaaaacccg gaactggggc ttatcaacaa ctggctgctg catatccgcg atatctggtt
    14761 caaacatagc tcattgctcg gcgaaatgcc gcaagagcgc cgtctggata ccttgtgtga
    14821 actgaacgtc atggaacagg tgtataacct gggccactcc accattatgc aatcagcgtg
    14881 gaaacgcggg cagaaagtta ccattcacgg ctgggcctat ggcattcacg acggcttgct
    14941 gcgtgatctg gatgttactg ccaccaaccg cgaaaccctt gagcaacgtt accgtcacgg
    15001 gatttccaac ctcaagctga aacacgccaa ccacaaataa aaatgccatg ccggatgcaa
    15061 cacatccggc aacttcacac ttactcgtcc agcagaatca ctttgccgat atacggcaga
    15121 tgacgataac gctgtgcgta atcaataccg taacccacca caaactcatc cgggatcgag
    15181 aaaccgataa attctaccgg gacgttcact tcacggcggg acggtttatc cagcagcgta
    15241 caaatcgcca gcgacttcgg ttcgcgcagg cttaagatct cacgcacttt cgacagtgta
    15301 ttccccgagt cgatgatatc ttcaacaatc agcacgtcct tgccacggat atcttcatcc
    15361 agatctttga ggattttcac atcacgggtg gtggacatgc cgctaccgta gctggaggcg
    15421 gtcataaagt cgacttcatg agatacctga acttcacggc acaggtccgc cataaacata
    15481 aatgagccac gcagcagacc caccagcacc atatcgctgc cgctgtcttt gtaacgctca
    15541 gtaatctgac gacccagttc ggcgatacgc gctttaatct ccgcttcggg gatcattact
    15601 tctacagtat gtttcatatc tctaaccata tgatttaaaa acaaatcact tcgcacgggc
    15661 aggttttggc accgcaacaa agcgcaagac acgcagtata ccagtaaaac tattcattcg
    15721 cggaatctgt taataaagct cgaattgtga tgacggtcac acatgttaaa acctataatt
    15781 aattgctact aaaatattaa tgaattgaaa tggtgtctct ttatggcaat taacaataca
    15841 ggctcgcgac gattactcgt cacgctaaca gccctttttg cagcgctttg cgggctgtat
    15901 ctactcattg gcggaggctg gctggtcgcg attggcggct cctggtacta ccctatcgct
    15961 ggccttgtga tgctcggcgt cgcctggatg ctgtggcgca gtaaacgtgc cgcgctttgg
    16021 ctatacgccg ccctgctgct cggcaccatg atttggggcg tctgggaagt tggtttcgac
    16081 ttctgggcgc tgactccgcg cagcgatatt ctggtcttct tcggcatctg gctgatcctg
    16141 ccgtttgtct ggcgtcgcct ggtcattcct gccagcggcg cagttgctgc gctggtggtc
    16201 gcactgctga ttagcggtgg tatcctgacc tgggccggat ttaacgatcc gcaggaaatc
    16261 aacggcacct taagcgccga tgccacacct gccgaagcta tctcacccgt tgccgatcag
    16321 gactggcctg cgtatggtcg taatcaggaa ggtcaacgct attcgcccct gaaacagatc
    16381 aacgccgata acgtccataa cctgaaagaa gcctgggtgt tccgtactgg cgatgtgaag
    16441 cagccgaacg atccgggtga aatcaccaat gaagtgacgc cgattaaagt gggcgacacc
    16501 ctttacctgt gtaccgctca ccagcgactg tttgcgcttg atgccgccag cggcaaagag
    16561 aaatggcatt acgatcctga gctgaaaacc aacgagtctt tccagcacgt aacctgccgt
    16621 ggcgtctctt atcatgaagc caaagcagaa acggcttcgc cggaagtgat ggcggattgc
    16681 ccgcgtcgta tcattcttcc ggtcaatgat ggtcgactga ttgcgattaa cgctgaaaac
    16741 ggcaaactgt gcgaaacctt cgccaataaa ggcgtgctca atctgcaaag caatatgcca
    16801 gacaccaaac cgggtctgta tgaaccgact tcgccaccga ttatcaccga taaaaccatt
    16861 gtgatggcag gttcagttac cgataacttc tcaacccgcg aaacgtctgg cgtgatccgt
    16921 ggttttgatg tcaacaccgg ggagctgctg tgggcttttg atccgggcgc gaaagatccg
    16981 aacgcaatcc cgtctgacga acacaccttt acctttaact cgccaaactc gtgggcacca
    17041 gcggcctatg acgcgaagct ggatctggtg tatctgccga tgggcgtgac cacgccggat
    17101 atctggggcg gtaaccgcac accggaacag gaacgttatg ccagctcgat tctggcgctg
    17161 aatgccacaa ccgggaaatt ggcgtggagc taccagaccg ttcaccacga cctgtgggat
    17221 atggatcttc cggcacagcc gacgctggcg gacatcaccg ttaatggtca gaaagtgcca
    17281 gttatttacg ctccggcgaa aaccggcaac atttttgtgc tcgatcgtcg taatggcgaa
    17341 ctggtggttc cggcaccgga aaaaccggtt ccccaaggtg ctgccaaagg cgattacgtt
    17401 acccctactc aaccattctc ggaactgagc ttccgtccga cgaaagattt gagcggtgcg
    17461 gatatgtggg gagccaccat gtttgaccaa ctggtgtgcc gcgtgatgtt ccaccagatg
    17521 cgctatgaag gcattttcac gccgccatct gaacagggca cgctggtctt cccgggtaac
    17581 ctgggaatgt ttgaatgggg cgggatttcc gttgatccaa atcgtgaagt ggcaattgcc
    17641 aacccaatgg cactgccgtt tgtttcgaaa ctgatcccac gcggtccggg taacccgatg
    17701 gagcagccga aagatgccaa aggcacgggt acggaatccg gcattcagcc acagtacggt
    17761 gtaccgtatg gtgtcacgct caacccgttc ctctcaccat ttggtctgcc atgtaaacag
    17821 ccagcatggg gttatatctc ggcgctggat ctgaaaacca atgaagtggt gtggaagaaa
    17881 cgtattggta cgccgcagga cagtatgccg ttcccgatgc ctgtaccggt gccgttcaat
    17941 atgggtatgc cgatgctggg cgggccaatc tccacggcgg gtaacgtgct gtttatcgcc
    18001 gctacggcag ataactacct gcgcgcttac aacatgagca acggtgaaaa actgtggcag
    18061 ggtcgtttac cagcgggtgg tcaggcgacg ccaatgacct atgaagtgaa tggtaagcag
    18121 tatgtggtga tctccgcagg cggtcacggt tcatttggta cgaagatggg cgactatatt
    18181 gtggcttatg ccctgccgga tgatgtgaag taagacttgc tcagattgct gacaacgtgc
    18241 gcgttgttca tgccggatgc ggcgtgaacg ccttatctag cctacaaaat tgtgcaaaat
    18301 caatggattg cacaggtagc gtaggcctga taagcgtagc gcatcaggca gatttgcgtt
    18361 tgtcatcagt ttaatgcccg gagagatccg ggcatattcc cgaatacggt ctttttatac
    18421 cgtaaaccct aacatcatcc ccgtatcttc atgctccagc agatggcagt gcgccatata
    18481 agcatgttct ttcggtgcgt cgtgattaaa cttcaccagc acttcactga cattgccttc
    18541 taccttaacg gtatctttcc agcccgcgcg atgagccgct ggcggtttgc cattttctga
    18601 caagatacgg aactgcgtgc cgtgaatatg gaacggatgc agcatcatgt cgcccacgcc
    18661 agagataacc caacgttcgt actgcccttt cgccgccgca aacatcggct tattcatatc
    18721 aaacgcctga ccgttgattt tattggcatg gtggaaatcg aacttcccgc cgtggttcat
    18781 atgattcata ttgccgtgcc ccatatggcc catcatctgg ctgtgatcca ttccggccat
    18841 cgcctgatcg ccatatttct ccattagcat ctgcatcccc atcatatcga gcatcgggtc
    18901 catagaaagt tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg caggcaggct
    18961 acttaatgtg tctggcaaag caccggaggc actaatagca atcggctgaa tccgcattac
    19021 cggatgcggc ttgtcaaacg gtgcaatcgc catccccatc tggctgaccg gcagcgtcac
    19081 caggtcaaag ggtttgttgt cgttaacctc caccagcact tcaaaacgct cgcccatcag
    19141 caccggcagc tcgttcacct tcaccggttc aggtagcaga ccaccgtcgc tggcaatcac
    19201 ataaagcggg cgattgtcgc tggtggcgaa attgagcgag cgggcattac agccattgag
    19261 caaacgcagg cgcagccaac cacgcggggc agcgtgttgc gggtagattg caccgttggt
    19321 cagcaacgta tcgccaaacc agcccacggc ggcggtcatc acatccagtt gataatcaat
    19381 ctgcccgtcg gcgctaaatt tcttatcctg aacgatcacc ggaacatcat cgatacccca
    19441 ctgttttggc agcattaatt tcaggatctc gtcatcttca atcaccacca gaccagccag
    19501 ccccatcgcc acctgtcgcc cggtcttgcc atgttgatgt ggatggaacc agcaggtagc
    19561 ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa taattccctg
    19621 tgggccgccg tcgacttcac ccggtacttc cagcccgtgc cagtgcaacg tcgtctcttc
    19681 cgtcagttgg ttatagatat caaccgttac cgctttgccg cgctgtaatt tcaccgccgg
    19741 ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa aggtagactg
    19801 acctgcgcca atagttaact gaatgcgatt acgagcatcg gtcgtgagca aatcagggat
    19861 cggtaacgtt gggcgttccg ccgcaaagac tgcacggctc cacagtggca aggctgaagc
    19921 cacacccagt gcgacggaat atttcaagaa atcacgacgt tgcatagtta tttccttatt
    19981 cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac gggaaggtca
    20041 agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag tgtgctaacg
    20101 tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac agttttattc
    20161 ggcagcctga tggccgtttg cgcaaacagt tacgcgctca gcgagtctga agccgaagat
    20221 atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta ccagaactta
    20281 cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca gtgggatctc
    20341 agtaattacg acaccttcga catgaaagcc ctcggtgaag acagctaccg cgatctcagc
    20401 ggcattggca ttcccgtcgc taaaaagtgc aaagccctgg ctcgcgattc cttaagcctg
    20461 cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg tgcatccgcg
    20521 gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag gaggtatcaa
    20581 cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg gcagtacttt
    20641 gcggtagata acgttctgta tcatgaaaag accgatcacc aggatctgat catttttgag
    20701 aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac cgagcgcgac
    20761 gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg tcacgcgaaa
    20821 catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac ccgacataaa
    20881 aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtatcgtt ctgccgtcag
    20941 tatctaccca accataacgc cggtagctac gacgatccgc gctttaagct ggtgatcgat
    21001 gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat ctccgactgc
    21061 accgatccta tcggtcccgg cgaaagcctt ttcacttcgg cattttatga aggctgcaaa
    21121 cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt tttacagcag
    21181 gaagaagcca tcgacagcca tcgcaaactc agccattact tcagcgacgt tggcttttat
    21241 caggcggcga tcccgaccta ttacggcggt atcatgactt ttgcatgggc gacagataac
    21301 gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc ctctggcctg
    21361 aaatgccgtt attacaatcc ggcaatccat acggcagctt ttgccttacc tcagtatctg
    21421 caagacgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa aaaactgaaa
    21481 ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga tatctgctac
    21541 gccaaaactg ccgaagagcg cgacggttat attgcttata tcgatgaact ctataatgcc
    21601 aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggctaa tattcttaac
    21661 atcgcccgcc aggattacga accacagggt gccagcgtca ctattctggt gagtgaagaa
    21721 ccggttgacc cgaaactcat cgacaaaaca gaacaccccg gcccactgcc agaaacggtc
    21781 gttgcccatc ttgataaaag tcatatttgc gtacatacct acccggaaag tcatcctgaa
    21841 ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt gatttctccg
    21901 ctgaaggcgc tgaattacct gatccaccag cttgagtccg atatcgtaac cattgattat
    21961 cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc actttatcga ccatgagatt
    22021 aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat ggtggatgtg
    22081 aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt cgaccttaag
    22141 cactacatgt tccacaccaa accggaagac ttaaccgaca gcgagcgcca ggaaattacc
    22201 gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc agctgtttaa
    22261 cggctctggc ggagctccca ggctccgcca gatttattta cttctgctgc acgaaattac
    22321 ggtaagccgc cacgacctgc agaaaatcct caacgccgca tagcgacagg ctttcttcgt
    22381 cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga ttggcgcgaa
    22441 ccatcacctc ttcaccgtcc atccacaggg tgtattcatg ccctgcccgt tgccaggacc
    22501 gttcgctacc tttcagtgcg tgcgctgctt gttccacttc atcaagcaag gccaggtttt
    22561 ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac atacgcacct
    22621 ttaccactcc ggtggtatcg cgcagaaatt cgtaatccat agtgtgttcc tctgctcccg
    22681 gcaatgcgcc gaaatcgcat tgcgccattg atgtaattat cgcagcctta ccggagaaaa
    22741 acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt caccgggcat
    22801 tgtgtcgttt atgcgcagcg cgtgcgctga ctttttaaac tgcagtctgg aaaatcacac
    22861 catcggcttt ttcagtgtac tgagaaagct ggttgaagtt caggtaacgg taagtatcaa
    22921 cggcagtttt atccacctgc gccacgtagg tctggtactc ttccggcgtc ggtagtttgc
    22981 caatcagcgc cgcaacagca gccagttccg cagaagccag gaagacattc gcgccagtcc
    23041 ccagacggtt cgggaagtta cgggtagagg tggaaaccac cgttgcaccg tccgccacac
    23101 gcgcctggtt acccatacac agggaacagc cagggatctc gatacgcgca ccactcttac
    23161 cgaagacgct gtagtagcct tcttcggtca actgtgcggc gtccatacgg gttggcggtg
    23221 ccacccacag gcgggtcggc aactggcctt tatgcgcatc cagcagttta cccgcagcac
    23281 ggaagtgacc gatgttggtc atgcaggaac cgataaacac ttcgtcgatc ttctcaccct
    23341 gtaccgcaga cagcggacgc gcgtcatccg ggtcgttcgg agcacacagg attggctctt
    23401 taatatccgc cagatcgatg tcgatcactg ccgcgtattc cgcatctgca tcggcttcca
    23461 gcagctcagg attcgccagc catttttcca tgccctgaat acgacgttcc agggtacgac
    23521 gatcgccgta accttccgcg atcatccact tcagcaggac gatattagag ttcaggtatt
    23581 cgatgatcgg ttctttgttc agcttgatgg tacaaccggc agcagaacgc tcggcggacg
    23641 catcggtcag ttcaaatgcc tgctcaactt tcagatccgg cagaccttca atttccagaa
    23701 tgcgacctga gaagatgttt ttcttgcctt tcttctcaac ggtcagcaga ccttgtttga
    23761 tcgcatacag cggaatagcg tgtaccagat cgcgcagggt gatgcccggc tgcattttgc
    23821 ctttgaagcg caccagaacg gattccggca tatccagcgg cattacgcct gttgcggctg
    23881 caaatgccac cagaccagaa cccgccggaa aagagatacc gatcgggaaa cgggtatggg
    23941 agtcaccacc ggtaccgacg gtatccggca gcagcatacg gttcagccag gagtggatta
    24001 cgccgtcacc cggacgcagc gacacaccgc cacggttcat aatgaagtcc ggcagcgtgt
    24061 ggtgcgtgtt cacgtcaacc ggcttcggat acgccgctgt gtggcagaaa gactgcatca
    24121 ccaggtcagc cgagaagccc aggcacgcca ggtctttcag ttcatcacgg gtcatcgggc
    24181 cggtggtgtc ctgggaacct acagaagtca ttttcggttc acagtacgcg cccggacgaa
    24241 tgcctttcac gccacaggca cggcctacca ttttctgcgc cagcgagaag ccgcgatcgc
    24301 tctcagcgac atctttcgcc tgacggaaca catcactgtg cggcagacca agtgcttcac
    24361 gcgctttggt ggtcaggcca cgcccgataa tcagcggaat acggccgcca gcacgcactt
    24421 catcaatcag cacgtcggtt ttcagttcga aggtcgccag cagttcgcct gtttcgtggt
    24481 tacgcacttc acctttgtac gggtaaacgt caatcacgtc gcccatgttc aggttagaga
    24541 cgtcgacttc gattggcagt gcacccgcgt cttccatcgt gttaaagaag attggtgcaa
    24601 ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga atatcatcgc
    24661 ccataaacca cagaacggag ttggtggcgg atttacgcga tgaacccgta cccacaacgt
    24721 caccgacgta cgccagcggg aaacctttct gttgcagagc ttcaatctgt ttgatcgggc
    24781 caacaacacc tggctggtct ggctcaatac cttcacgggc gtttttcagc atcgccagcg
    24841 cgtgcagtgg gatatccggg cgcgaccacg catccggtgc cggagaaagg tcatcggtgt
    24901 tggtttcgcc agtgactttg aagacggtaa cggtcagttt ttcagccagc gccgggcgat
    24961 tcaggaacca ttcggcatcc gcccaggact gcattacctg cttcgcatat tcgttgcctg
    25021 ctttggcttt ctcttctacg tcatagaagt tatcgaacat cagcagcgtg tgagaaagtg
    25081 ctttggcagc gatcggtgcc agtttggcat catccagcgc gtcgatcagc ggatgaatgt
    25141 tgtaaccacc ctgcatggtg cccagcagtt cgatggcttt ttccggagtc agcagtgggg
    25201 atttggcttc acctttcgcg acagcagcca ggaagcctgc tttgacatag gcggcttcat
    25261 cgacgccagg gggaacacgg ttggttaata gatctaacag gaattctttt tcgcccgcgg
    25321 gcgggttttt cagcagctct acaagtgcgg ccatttggtt tgcatccagg ggtttgggcg
    25381 caatcccctc agcggcacgc tcagctacgt gcttacggta ttcttctagc acgacggttc
    25441 tcctcgctct cattgtcata gtgcggcagg cgattctctt cacgctcctg tgagacagca
    25501 gtttgtaggg taaatgccca gtaccgcaac gggcagaata gcaggatttt gacgatgtgt
    25561 taatctgttt acaaaaaagc aacataaaaa agtggctgaa tcgttaagga tggtctagag
    25621 attgtttccc tccgtaaaaa cttcgttaat tcgcatagtg ataatcacct ttctcaacga
    25681 atcccaagat tagtcaaaat ttaaactacc gcctctttat actcagattt acagcacctg
    25741 cgggtggcag ttcgccgacc attgcgattt ccttgagatc cgaattatga aaatgacttt
    25801 gccgtttaaa ccccatgtgc tggcgctaat ttgcagtgcc gggctttgtg ccgcctctgc
    25861 cgggctatat ataaaaagcc gcacagtgga agcgcctgtg gaaccgcaat cgacacaaca
    25921 gactgcgcct gacatcaccg cagttacgct tcctgcaacg gtttccgcac cgcccgtaac
    25981 gcccgccgtc gttaaatccg cattcagcac tgcacaaatc gatcaatggg ttgcacctgt
    26041 cgcgctatac cccgactctc tgctttcaca agtgttaatg gcatcaacct atccggcaaa
    26101 cgttgctcaa gcagtgcaat ggtcgcacga taatccactt aaacaaggcg atgctgctat
    26161 tctggcggta tctgaccagc cgtgggacgc tagcgtcaaa tcactggtgg cctttccaca
    26221 attgatggcg ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg atgcttttct
    26281 ggcgcagccg caggacgtga tggactcggt acagcgattg cggcaactgg cgcaacaaac
    26341 cggctcgctg aagtcatcaa ccgaacagaa agttattacc acaacgaaga aaaccgtacc
    26401 ggtaacacag acagtcacgg ctcccgtcat accatccaat accgtttcaa ctgccaaccc
    26461 cgtcattaca gagcctgcaa caaccgtcat ttccattgag cccgccaatc ctgatgtggt
    26521 ctatattccc aactacaacc caaccgtggt ttacgggaac tgggccaata ctgcgtatcc
    26581 gccggtttat ctgccaccac cagccggaga accgtttgtt gacagctttg tgcgcggatt
    26641 cggctatagc atgggcgttg ctaccacgta cgcactattc agcagcatcg actgggatga
    26701 cgacgatcat gaccatcatc atgacgatga ttatcatcac cacgatggcg gtcatcgtga
    26761 cggtaatggc tggcaacaca acggcgacaa catcaatatc gacgtcaaca atttcaaccg
    26821 tatcaccggt gagcatctta ctgataagaa tatggcatgg cggcacaatc caaactaccg
    26881 taatggtgtg ccctatcatg atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa
    26941 cggcggaatg agtgccacgc agctacctgc tccaacacgc gatagccagc gtcaggcggc
    27001 agcaagtcag tttcagcaac gaacacacgc cgcccccgtc attacacgag atacccaacg
    27061 tcaggcagcg gcacagcggt ttaatgaagc tgaacactat gggagctatg acgacttccg
    27121 cgacttcagc cgtcgccaac cactgaccca gcaacaaaag gacgccgctc gtcagcgtta
    27181 tcagtcagct tctcctgagc cgcgccaggc agttcgcgag agaatgcaga ctaacccgca
    27241 gaaccagcag cgaagagagg cagcgcgtga gcgcattcag cccgcctcgc ctgagcagcg
    27301 ccaggcagtc cgcgagaaaa tgcagactaa cccacagatc cagcagcgaa gagaggcagc
    27361 gcgtgagcgt attcagtcag cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca
    27421 gcagagccca ctgaaccaac agcaacgtga taacgcccgc cagcgtgttc aatcggcatc
    27481 acctgaacaa cgtcaggttt ttcgggagaa agttaaggag agccacccac aacgtctaaa
    27541 cgacagtaac catactgcca ggctaaataa cgagcaacgg tcagcagtac gcgaacgtct
    27601 ctctgagcgc ggagcaaggc gactggaaag gtaaattaca gacgtaaaaa aagcggcgtg
    27661 gttagccgct tttttaattg ccggatgttc cggcaaacga aaaattactt cttcttcgct
    27721 ttcgggttcg gcaggtcggt aatgctacct tcgaacactt ctgccgccag gcccacagac
    27781 tcgtgcagag tcgggtgcgc atggatggtc agcgcgatgt cttcagcgtc acaacccatt
    27841 tcgattgcca ggccgatttc acccagcagc tcgccgccgt tggtaccgac aatcgcacca
    27901 ccgatcacac ggtgagattc tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg
    27961 gaagcgatag cacgaccaga agcagcccac gggaaggtgg cggtttcata gctgatgcct
    28021 ttctctttcg cttctttctc agtcagacct acccatgcaa cttctggttc ggtataggcg
    28081 atggacggga taactttcgg atcgaagtag tgtttcttac cggcgataac ttcagcggca
    28141 acgtgacctt cgtgaacacc tttgtgtgcc agcatcggct gaccgacgat atcgccgata
    28201 gcaaagatgt gcggtacgtt ggtacgcagc tgtttgtcaa cgcggatgaa accacgatcg
    28261 tcaacttcca cgccagcttt gcctgcgtcg aggtttttac cgttcggcac acgaccaatc
    28321 gctaccagca ctgcgtcgta acgctgcggt tcagctggtg cttttttgcc ttccatcgtc
    28381 acataaatac cgtcttcttt cgcttcaacg gcggtaactt tggtttccag catcaggttg
    28441 aatttcttgc tgatacgctt ggtgaagact ttaacgatgt ctttgtcagc tgccgggata
    28501 acctggtcga acatttcaac cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc
    28561 atttccagac cgatgatacc gccacccatt accagcaggc gttctggtac ttctttcagt
    28621 tccagcgcgt cagtggagtc ccagatacgc ggatcttcat gcggaataaa cggcagttgg
    28681 atcgggcgag aacccgctgc aatgatcgcg ttgtcgaagt tgatcacggt tttaccgttc
    28741 tcaccttcaa cttccagggt gttagccccg gtaaatttac ccagaccgtt gaccactttg
    28801 actttgcggc ctttcgccat accagccaga ccaccggtca gctgattgat cactttctct
    28861 ttccaggtac gaatcttgtc gatatcggtt ttcggttcgc cgaagacgat accgtgttca
    28921 gccagcgctt tggcttcttc gataactttt gctacgtgca gcagtgcttt agaagggata
    28981 cagccgacgt tcaggcaaac accgccaagg gtgttgtaac gttctacgat tacggtttcc
    29041 agacctaaat cagcgcaacg gaaggcagcg gagtaacctg cggggcctgc cccaagtacc
    29101 acgacctgag ttttgatttc agtactcatc atgacctcta tatatttatc tccggcgggt
    29161 ctgacgtttt ttttatgacg cccattatcc accgggtcgt tctatccgtc ggtattttac
    29221 aaaattgtta acaattttta aacaacaaac ggcaaccgat ttgtctattc gctaataacc
    29281 tcaatacatt catgagatta ccagaaaaaa gccggccgtt gggccggctc ttttacttac
    29341 atcaccagac ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa acgggcacca
    29401 tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat cagacgcggc
    29461 acgaactctt taccattcca caccggctcc atcgcggact tggaaacgcc gaggatagcc
    29521 acttccggcg cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc gatgctggag
    29581 atggtgaagc aaccgccctg catttcgccc gcagtcagct taccgtcacg cgctttctta
    29641 gaaatagtca tcagctcgcg agacagctcg atgatgcctt tcttgttgac gtctttgaat
    29701 accggaacaa ccaaaccgtt cggggtatcc accgccacac cgatgttgat gtatttcttc
    29761 agggtcagac gctgaccgtc ttccgacagc gaactgttga agcgaggcat ctgctcaaga
    29821 gctgcagcaa cggctttcat gatgaagaca accggggtga tcttcacatc cagcttacgt
    29881 ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca gctcggtgat atcggttttg
    29941 tcgaagtgag taacatgcgg gatcatcacc cagttacggc tcaggttcgc accagaaatt
    30001 ttctggatac ggcccagttc cacttcttcg atttcaccaa acttgctgaa gtccaccttc
    30061 ggccacggca gcatgcctgg gataccgccg ccagtcgccg ccggagctgc ttctgcacgt
    30121 ttgatagctt ctttcacgta agcctgaacg tcttcgcgca ggatacgacc tttacggcca
    30181 gtgcccttca ctttcgccag gttaacgcca aactcgcgtg ccagacggcg gatcagcgga
    30241 gtcgcgtgaa cataagcgtc gttttcagca aattcagatt tgccttccgc tttcgcagct
    30301 ggtgctgctg ccggggcttc agcttttgct gccggagccg gcgctgccgc ttcctgtttc
    30361 gcaggagctg ccgcaggcgc tgcgccttca acttcgaaga tcataatcag cgagccagtt
    30421 ttcactttat cgccaacgtt gactttcagt tccttcacga cgcctgcaaa cggagccgga
    30481 acttccatag aagctttgtc gccttctacg gtaatcagtg actgttcagc ggcaactttg
    30541 tcgcccactt tcaccatcac ttcggtcact tcaacttcgt caccgccgat atccggaacg
    30601 ttaacttctt tcacgccagc tgctggtgca ggggccgctg ccggagccgc ttcctgttta
    30661 gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga agaccataat cagcgagccg
    30721 gtagacactt tgtcgccggt gttcactttg atttctttca cggtgcctgc aaacggagcc
    30781 ggaacttcca tagaagcttt atcgccttct acggtgatca gcgactgctc agcttcaact
    30841 ttgtcgccaa ctttcaccag gatttcggtc acttcaactt cgtcgctgcc gatatccgga
    30901 acgttaacgt cttttgccgc ggcagccgcg ggtgctgctg ccggagctgc ttctttcttc
    30961 tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc
    31021 agtgcgccgg tctgggtttt atcgccaaca gagactttga tctctttaac gatacccgcc
    31081 tgcggagacg gaacttccat agaggctttg tcgccttcta cggtgatcag cgactgttcg
    31141 gcttcaactt tgtcgcccac tttgaccagg atctcggtga tttcaacttc atcagccccg
    31201 atgtccggta ctttgatttc gatagccatt attcttttac ctcttacgcc agacgcgggt
    31261 taactttatc tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact ttcttatcga
    31321 tttcgccacg tttagccagt tcgcccagcg ccgcaaccac gacataagaa gcatcaactt
    31381 cgaagtggtg acgcaggttc tcacggctgt cggaacgacc gaagccatca gtacccagta
    31441 cgcggtagtc gtcagccggt acgtaagtac ggacctgctc agcgaacagt ttcatatagt
    31501 cggtagatgc cactgccgga gcgtcgttca tcacctgcgc gatatacgga acgcgcggag
    31561 tttccagtgg gtgcagcatg ttccagcgtt cacaatcctg accatcacgc gccagctcgg
    31621 tgaaggaggt cacgctataa acgtcagaac ctacgccgta atctttcgcc aggatctctg
    31681 ccgcttcacg gacgtgacgc aggatagaac cggagcccag cagctgaact ttacctttgc
    31741 taccttcaat ggtttcgagt ttgtagatac ctttacggat accttcctca gcaccttccg
    31801 gcattgccgg catgtggtag ttttcgttca gcgtggtgat gtagtagtaa acgttctctt
    31861 gtttttcacc gtacatacgc tccagaccgt catgcataat gacagcaact tcgtaagcgt
    31921 aagccgggtc gtaagagata cagttcggaa tagtcagcga ctgaatatgg ctgtgaccat
    31981 cttcgtgctg cagaccttca ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga
    32041 agccacgcgc ttgctggtcg ccagccgccc agcacagatc gccgatacgc tggaagccga
    32101 acatcgagta atagatgtag aacggaatca tcggcagatt gttggtgctg taagaggtcg
    32161 ccgctgccag ccaggaacaa cctgcgccca gctcgttgat cccttcctgc agaatctgac
    32221 ctttctcgtc ttctttatag taagcaacct gctcgcggtc ctgcggggtg tactgctgac
    32281 cgttcgggct gtaaatacca atctgacgga acagaccttc cataccgaaa gtacgcgctt
    32341 cgtcggcgat gatcggtacc agacgatctt tgatcgactt gttcttcagc atcacgttca
    32401 gggcacgaac gaaagcgata gtggtagaga tctctttgct ttgctcttcc agcagcgcgc
    32461 cgaagtcttg caggctcggc agctcaagct tctcggtgaa gttcggctga cggcttggca
    32521 gataaccgtg cagtttctga cgctgagcgt gcagataggt atgctcttca gaaccttccg
    32581 ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga caccggcaca ttgaaacggt
    32641 cgcggatatg acgcacaccg tccatgttca ttttcttaac ctggtgcgcg atgtttttac
    32701 cttcagccgc gtcgcccatg ccgtaacctt taatggtatg agcaaggatt actgtcgctt
    32761 tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac
    32821 cacggttcag tgcccagatc tgctcgtcag tccagtctgc aaccagtgct gcggtttcag
    32881 gatatttacc gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg aaggtctggt
    32941 agtcgccgtc aacggtttcg ttcatcagct ggatcagttt accgctggta tctttacgca
    33001 gcagttcatc ccaacggcta ccccacatca ctttgatcac gttccagcca gcaccttcga
    33061 agatgccttc cagttcgttg atgatcttgc cgttaccggt gaccgggccg tcaagacgct
    33121 gcaggttaca gttgataacg aagaccaggt tatccagttt ttcacgggta gcgatggtga
    33181 tcgcaccttt ggattccggt tcgtccattt caccgtcgcc gaggaacgcg taaacggtct
    33241 gtttagaggt atctttcagg ccacggtgtt ccagatattt caggaattta gcctggtaaa
    33301 tagcaccaat cggacccaga cccatagata cggtcgggaa ctgccagaat tccggcatca
    33361 gtttcgggtg cggataggaa gagaggccat tgccgtgaac ttcctgacgg aagttatcca
    33421 gctgctcctg agtcagacga ccttccagga aagcacgagc gtaaacgccc ggggagatgt
    33481 ggccctggaa gtaaaccaag tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt
    33541 taaagcacac atcataaatg gttgcggaag actggaagga cgccatgtgg ccgcccagtt
    33601 cgaggtcttt tttcgacgca cgcagaaccg tcatgatggc gttccagcgg atagctgaac
    33661 gaatacggcg ttccagttcc agattacccg gatactccgg ttgttcttca acggggatgg
    33721 tgttgatgta gttgctgata ccggtgcctg cggctacgtt gacaccgcct ttgcgggctt
    33781 cagcaagcag ttggtcgatc agatactgag cacgctcaac accttcttca cggatgaccg
    33841 attcgatcgc ctggagccag tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt
    33901 ctgacatggg ttattcctta tctatctaat aacgttgagt tttctggaac ctgtcccatt
    33961 gaactctcgc cggaaagctc aataagacag gttctacgtt tagttgccgc gcttttatat
    34021 gcgcttgatt tacaacatct tctggataat ttttaccaga aaaatcacta attctttcgt
    34081 tgctccagac gacgcagaga acgctcacgg cggctctctt cacggcttct gtcgagcaaa
    34141 atttcttcga taaaggccag atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg
    34201 gccataattg cttcgaatat acgggtgcgg tgactactca ccagcggcag catctcgcga
    34261 cgcgcataga gcaattcgaa gttctgacgg acattctggg ccaacatcgg ctccatacac
    34321 cttagcagat gaagcaaaac cacattgtgg gctgcttcgg tgacggcaat ctgatactgg
    34381 agtacggcgt ttgattccgc gtccagatcg ccagactgct gcgccagttc tatggcgtgg
    34441 tggagttcac ggatgcgttc cttgtcttca tcggtactac gcagcgcggc gtaataagcg
    34501 gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat actgtgattc aggatggtcg
    34561 gagagcagct ctaccagcgg atcgctgaag ctttgccata ggctgctctg gacaaaagtg
    34621 ccgccaccct ggcgacgaag caacaagccc ttcgcttcga gacgttgaat cgcctcacgc
    34681 aaggagggac gggagacgtc aaactgtttt gccagttcgc gttccggtgg gagtttttcg
    34741 cccgggcgga gagtgccttc gaggatcaaa aactccagtt gctgctcaat cacatcggag
    34801 agttttggtt ggcggatttt gctgtaggcc atgagttcct gtcttaagcc acttgccgaa
    34861 gtcaattggt cttaccaatt tcatgtctgt gacgctaaag taacaaagta ttcaccttat
    34921 gtccatacag gttttgattg aaatcatgaa actgtgcaca ttttaacaac ttgacatata
    34981 taacgtttca aagttgtaac tatgcacaaa tgtagacttt acg
//
LOCUS       DAZGBC010000032        34952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGBC010000032 DAZGBC010000000
VERSION     DAZGBC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.032"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..832
                     /locus_tag="ACHK1J_001602"
     CDS             <1..832
                     /locus_tag="ACHK1J_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO7910894.1"
                     /translation="NQNKIVKAWLLAIDDFEKEFEVNKNEMLLSIVNEIDSTYDLNSA
                     IKTNDYAVVNILLANIKAKMFKNEINKEDILKLMAAREKVAGASDKWTKASGLYSAIV
                     KGHTEIVAAWMETAEVIASHYENDKDVVRELLSLSRNNAVCSLYVASYKTMSKQVIDV
                     YLNAAIRLALQHGFTFDEIVEQFTRDFDGKSFSHVVEKADDIYMGLWLKIFKIVVGEN
                     ENYLKDVMMQLEAKNNEGKSVISQANGNPVFKELFWKAIDEFNFPQEELNRLNQYRSL
                     "
     gene            complement(971..2335)
                     /gene="mnmE"
                     /locus_tag="ACHK1J_001603"
     CDS             complement(971..2335)
                     /gene="mnmE"
                     /locus_tag="ACHK1J_001603"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO7910895.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2441..4087)
                     /gene="yidC"
                     /locus_tag="ACHK1J_001604"
     CDS             complement(2441..4087)
                     /gene="yidC"
                     /locus_tag="ACHK1J_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO7910896.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(4090..4347)
                     /gene="yidD"
                     /locus_tag="ACHK1J_001605"
     CDS             complement(4090..4347)
                     /gene="yidD"
                     /locus_tag="ACHK1J_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO7910897.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(4311..4670)
                     /gene="rnpA"
                     /locus_tag="ACHK1J_001606"
     CDS             complement(4311..4670)
                     /gene="rnpA"
                     /locus_tag="ACHK1J_001606"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO7910898.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4687..4827)
                     /gene="rpmH"
                     /locus_tag="ACHK1J_001607"
     CDS             complement(4687..4827)
                     /gene="rpmH"
                     /locus_tag="ACHK1J_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO7910899.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(5057..5137)
                     /locus_tag="ACHK1J_001608"
     CDS             complement(5057..5137)
                     /locus_tag="ACHK1J_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910900.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            5434..6837
                     /gene="dnaA"
                     /locus_tag="ACHK1J_001609"
     CDS             5434..6837
                     /gene="dnaA"
                     /locus_tag="ACHK1J_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO7910901.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6842..7942
                     /gene="dnaN"
                     /locus_tag="ACHK1J_001610"
     CDS             6842..7942
                     /gene="dnaN"
                     /locus_tag="ACHK1J_001610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO7910902.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7942..9015
                     /gene="recF"
                     /locus_tag="ACHK1J_001611"
     CDS             7942..9015
                     /gene="recF"
                     /locus_tag="ACHK1J_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO7910903.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            9044..11458
                     /gene="gyrB"
                     /locus_tag="ACHK1J_001612"
     CDS             9044..11458
                     /gene="gyrB"
                     /locus_tag="ACHK1J_001612"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO7910904.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11482..11580)
                     /locus_tag="ACHK1J_001613"
                     /pseudo
     CDS             complement(11482..11580)
                     /locus_tag="ACHK1J_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11698..12096
                     /gene="yidB"
                     /locus_tag="ACHK1J_001614"
     CDS             11698..12096
                     /gene="yidB"
                     /locus_tag="ACHK1J_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO7910905.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGDVAPQANNDLLSAGMELLKGKLFR"
     gene            12211..13023
                     /gene="yidA"
                     /locus_tag="ACHK1J_001615"
     CDS             12211..13023
                     /gene="yidA"
                     /locus_tag="ACHK1J_001615"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO7910906.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(13069..13725)
                     /gene="yidX"
                     /locus_tag="ACHK1J_001616"
     CDS             complement(13069..13725)
                     /gene="yidX"
                     /locus_tag="ACHK1J_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO7910907.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGTSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13861..13974
                     /locus_tag="ACHK1J_001617"
                     /pseudo
     CDS             13861..13974
                     /locus_tag="ACHK1J_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13971..15035)
                     /gene="cbrA"
                     /locus_tag="ACHK1J_001618"
     CDS             complement(13971..15035)
                     /gene="cbrA"
                     /locus_tag="ACHK1J_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO7910908.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDNKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFICGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            15136..16350
                     /gene="yidR"
                     /locus_tag="ACHK1J_001619"
     CDS             15136..16350
                     /gene="yidR"
                     /locus_tag="ACHK1J_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO7910909.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGEMVSFTYNDHVMHERSPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCALVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPKDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAESGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16352..16684)
                     /gene="yidQ"
                     /locus_tag="ACHK1J_001620"
     CDS             complement(16352..16684)
                     /gene="yidQ"
                     /locus_tag="ACHK1J_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7910910.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASASM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16924..16996
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16990..17403
                     /gene="ibpA"
                     /locus_tag="ACHK1J_001621"
     CDS             16990..17403
                     /gene="ibpA"
                     /locus_tag="ACHK1J_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO7910911.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17515..17943
                     /gene="ibpB"
                     /locus_tag="ACHK1J_001622"
     CDS             17515..17943
                     /gene="ibpB"
                     /locus_tag="ACHK1J_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO7910912.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            18139..19800
                     /gene="yidE"
                     /locus_tag="ACHK1J_001623"
     CDS             18139..19800
                     /gene="yidE"
                     /locus_tag="ACHK1J_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO7910913.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19797..20523)
                     /gene="yidP"
                     /locus_tag="ACHK1J_001624"
                     /pseudo
     CDS             complement(19797..20523)
                     /gene="yidP"
                     /locus_tag="ACHK1J_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            20819..22435
                     /locus_tag="ACHK1J_001625"
     CDS             20819..22435
                     /locus_tag="ACHK1J_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7910914.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22435..23757
                     /locus_tag="ACHK1J_001626"
     CDS             22435..23757
                     /locus_tag="ACHK1J_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO7910915.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23754..24647)
                     /gene="yidL"
                     /locus_tag="ACHK1J_001627"
     CDS             complement(23754..24647)
                     /gene="yidL"
                     /locus_tag="ACHK1J_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7910916.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24814..26529
                     /gene="yidK"
                     /locus_tag="ACHK1J_001628"
     CDS             24814..26529
                     /gene="yidK"
                     /locus_tag="ACHK1J_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO7910917.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26526..28019
                     /gene="yidJ"
                     /locus_tag="ACHK1J_001629"
     CDS             26526..28019
                     /gene="yidJ"
                     /locus_tag="ACHK1J_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7910918.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYQLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVNTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEDKKQKF"
     gene            complement(28066..28515)
                     /gene="yidI"
                     /locus_tag="ACHK1J_001630"
     CDS             complement(28066..28515)
                     /gene="yidI"
                     /locus_tag="ACHK1J_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO7910919.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28624..28971
                     /gene="yidH"
                     /locus_tag="ACHK1J_001631"
     CDS             28624..28971
                     /gene="yidH"
                     /locus_tag="ACHK1J_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO7910920.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28961..29323
                     /gene="yidG"
                     /locus_tag="ACHK1J_001632"
     CDS             28961..29323
                     /gene="yidG"
                     /locus_tag="ACHK1J_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO7910921.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29320..29817
                     /gene="yidF"
                     /locus_tag="ACHK1J_001633"
     CDS             29320..29817
                     /gene="yidF"
                     /locus_tag="ACHK1J_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO7910922.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDTLQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     HPRRDCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29825..31009)
                     /gene="emrD"
                     /locus_tag="ACHK1J_001634"
     CDS             complement(29825..31009)
                     /gene="emrD"
                     /locus_tag="ACHK1J_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO7910923.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            31091..31165
                     /locus_tag="ACHK1J_001635"
     CDS             31091..31165
                     /locus_tag="ACHK1J_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910924.1"
                     /translation="MNVSQICARNGEKKNGRICKQKRQ"
     gene            complement(31289..31378)
                     /gene="tisB"
                     /locus_tag="ACHK1J_001636"
     CDS             complement(31289..31378)
                     /gene="tisB"
                     /locus_tag="ACHK1J_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO7910925.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31943..32041
                     /gene="ivbL"
                     /locus_tag="ACHK1J_001637"
     CDS             31943..32041
                     /gene="ivbL"
                     /locus_tag="ACHK1J_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO7910926.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            32147..33835
                     /gene="ilvB"
                     /locus_tag="ACHK1J_001638"
     CDS             32147..33835
                     /gene="ilvB"
                     /locus_tag="ACHK1J_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO7910927.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPVRVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33839..34129
                     /gene="ilvN"
                     /locus_tag="ACHK1J_001639"
     CDS             33839..34129
                     /gene="ilvN"
                     /locus_tag="ACHK1J_001639"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7910928.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34392..>34952
                     /locus_tag="ACHK1J_001640"
     CDS             34392..>34952
                     /locus_tag="ACHK1J_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910929.1"
                     /translation="MASPINSGIMMSNLCSSTFLDKNINVAAELDITNSEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGGKSFKVIQFDAGEKNTKKIVKETSYSGVAVATIIKSLSEKTAALETHSADTVLRK
                     KLVNSIVMKNTDFKHVITADIENNIYD"
BASE COUNT         8504 a         8789 c         9213 g         8446 t
ORIGIN      
        1 taatcaaaat aaaattgtca aagcatggtt gttggcgata gatgactttg agaaagaatt
       61 tgaggtaaat aaaaatgaaa tgcttcttag tatagtaaat gaaatagact caacttatga
      121 tttaaatagc gctattaaaa ccaatgatta tgctgttgtt aatatattat tagccaatat
      181 aaaagccaaa atgtttaaaa atgaaataaa taaagaagat atattgaaac tgatggcagc
      241 aagagaaaaa gtggcgggag catcagacaa atggacgaag gcatcaggct tatattctgc
      301 gatagtgaaa gggcatacgg agattgttgc tgcctggatg gagacagctg aagtgatagc
      361 cagccattat gaaaatgata aagatgtagt gagagaacta ctgtcgctga gcagaaataa
      421 tgcagtttgc tctttgtatg ttgccagcta taagacaatg agtaagcagg tcattgatgt
      481 atatctgaat gcggcgattc gcctggcgtt gcaacacggg ttcactttcg atgaaattgt
      541 ggagcagttt acccgtgact ttgatgggaa gtcattctct catgtggtag agaaagcgga
      601 tgatatatat atgggactct ggctgaaaat attcaaaatt gtggttggtg aaaatgaaaa
      661 ttatcttaaa gatgtgatga tgcaactgga agcgaagaat aatgaaggta aatctgtaat
      721 aagtcaggca aatggcaatc ctgtatttaa agaactattc tggaaagcaa tagacgaatt
      781 taactttccg caagaggagc ttaatcggtt aaaccaatat cgttctctgt aaagacagga
      841 ctgcaagatg ttgaaagtcg ctccagccag tagtaaaggc agttttggtg caggatgctc
      901 ataaaaaaac aagcgctgtt ccgttctgat agtagaacgg aacagtgccc tttatatcaa
      961 ttaagacgac ttacttacca atacagaagc tggagaaaat ccgccccagc aggtcgtcag
     1021 aggtaaattc cccggtgatt tcgcttaagt tctgctgcgc caggcgcaac tcttccgcca
     1081 gtagttcgcc tgcccaggca cctaacagtt gcgctttacc ttgctgcaaa tgctccgctg
     1141 cctgttccag cgcctgtagg tggcgacggc gcgccaggaa gccgccttcc atgttggtgt
     1201 caaagcccat gctctgtttg agatggttac gcagcacgtc cacgccttcg ccagtccttg
     1261 cagacagacg aattaacgcg tgaccgttca cttcacttat tcccagcgtt tcgccggtga
     1321 tatcggcttt attgcgcacc acggtgatcg gcagtttcgc tggcagacgg gcgataaatt
     1381 ccggccagat ctctgccgga tcaacggcgt ctgtcgtggt gccatcgacc ataaacagca
     1441 cgcggtcggc ctgttcaatt tcctgccacg cgcgctcgat cccaatacgt tcaacttcgt
     1501 cactggcttc acgtagcccg gcggtatcga tgatatgcag cggcattccg tcaatatgga
     1561 tatgctcacg cagcacgtca cgcgtggttc cggcgatatc ggttacgatt gccgcttcac
     1621 ggcccgccag cgcgtttaac aggctcgatt taccggcgtt aggacgtccg gcaatcacca
     1681 ccttcatccc ttcgcgcaac aaactaccct gacgtgcttc agcacgcact gcatcgagat
     1741 cggcaatcac gtcattaagc tgggcttcaa tttttccgtc ggagaggaaa tcgatctctt
     1801 catcggggaa atcaattgcc gcttcgacgt aaatgcgcaa gtgggtgagg gcttccacca
     1861 gatggttgac ccgtgcggag aatgcgcctt gcagcgagtt aagtgccgaa cgggctgcct
     1921 gttccgaact ggcatcgata agatcggcaa tcgcctcggc ctgggctaag tcgagtttat
     1981 cgttaagaaa cgcgcgttcg gaaaactcac cagggcgagc aatccgcagg ccgggaatgg
     2041 tcagaatgcg ttttaacagc aggtcgagga tcaccggacc gccatgaccc tgcagttcca
     2101 gcacatcttc gccggtgaac gagttcgggc cagggaacca tagcgcaatc ccctgatcga
     2161 gcacgctgcc gtcggcgtct ttaaacggaa gataatcggc gtagcgcggc ttaggcagtt
     2221 tacccagcac ggtttcggca acttcacggg ctttgaagcc ggagatgcgc aggatgccaa
     2281 cgccgccacg tcccggaggc gtggcctggg ctacgatagt gtcattatcg ctcatgatgt
     2341 tcctgttgct ttgtgtggcg gatgcgcggt gcttatccgc cctacgaaaa gaaaaaaggc
     2401 ggtcaactga ccgcccttat tttagcgaaa actcaccgaa tcaggatttt ttcttctcgc
     2461 ggctatgcag gccacgtttt tccagaccac ggtaaatcag ctgctgctga ataatggtta
     2521 ccaggttgct gacgatatag tacagcacca gacctgacgg gaaccacagg aagaacacgg
     2581 tgaagatgac cggcataaag gtcatgatct tctgctgcat cgggtcggtc acagtggtcg
     2641 gcgacatctt ctgaatgaag aacatcgtta cgcccatcag gatcggcagg atgtagtacg
     2701 ggtcctgtgc agacaggtcg tggatccaca gtgcgaacgg cgcctgacgc agttcaacgg
     2761 aacccatcag catgtagtac aacgccagga agattggcat ctggatcagc agcgggaagc
     2821 agccgcccag cgggttaacc ttctcagctt tgtacagcgc catcatttcc tggctgatac
     2881 gctgtttgtc atcgcccaga cgctcacgca tcgcctgaat cttcggctgc agcatacgca
     2941 tcttcgccat ggaggtgtac tgcgctttgg tcagcgggta catgatgcca cgaacgataa
     3001 aggtgatgat gataatggag aagccccagt tacccacaaa gctatggatc catttcagca
     3061 gtttgaacag cggctgagag atgaaccaca accaaccgta atcaacggtc agatccaggt
     3121 gcggagcaac agctgccatt ttgtcctgga tttccgggcc aacccacagg gtgctgttca
     3181 tcgcgccagt ctgaccaggc tgaaccagta ccggctgaga tttatagccg atagcggcga
     3241 tgccgttacc cagattagca gtatagaagt tgttggtacc gtcgttatgc gggatccacg
     3301 ccgtcgcgaa atactgttgc agcattgcca cccaaccacc tttcgaagag atgttcaggt
     3361 tttcgttatc ggcaatggta tcgaacttgt atttctcata cttctcgtca ggcgtggagt
     3421 acgccgcgcc acggaaggtg tgcagtgcga agttgctgct tccggtatcg agatgcggtg
     3481 gcagagtgat ggattgcttc aactgaccaa atgaggagat ttccagcggt ttctcgccag
     3541 cattctgcac gttgtagttg acgttgacag cgtaatcacc acgttttagg acaaacgttt
     3601 tggtaaacgt gttgcctgcc gcgtcggtat acgtcatcgg cacctgcagt tcgttttgac
     3661 cttcagccag cacataagcg tctttttcaa cgttatacag cggacgcggg ccgttagccg
     3721 ggttatccgg gccatcacga ccggtcagac cgctctgtgc ctgataaata aactgcggtg
     3781 aagtttccag cagctggaac ggctgggtag agttcagctc tttcgggtaa gcaggcagca
     3841 gagcttgctc aacatcacca ccacgggtgt tgatggtcag atcaagcacg tcggtcttaa
     3901 ccgagatcag tttcccctgg ccactggccg gtacgccctg gtcggcggcg ctacccgctg
     3961 cggtggtcgt tgtctgcgtg gtctgttggg cctgaggttg cgggttttta tcctgctccc
     4021 aggcttgcca gatcatgaaa gacacgaaca gcaaagcgat gactaaaaga ttgcgttgcg
     4081 aatccatcgt tagtgttctc tggtatcaaa tggtccgggc gggacgggat cgtcaccacc
     4141 agggtgtaaa gggtggcatt ttaatacgcg tttcaccgtc aaccaactgc cttttatcac
     4201 tccaaacctg cgcaatgcct caattccgta gcttgaacag gttggagtga aacgacaatg
     4261 cggcccgagt agcggactaa tcaggcgttg atagacccga atgagggcta tcaggacccg
     4321 cgagccaggc gacagtggcg gcgccataat ttttccaacg cttccgagag agcacggtta
     4381 tcgaggtcgg caaccccttt tttcgccacc accacgaaat ccatagccgg gagttcatgt
     4441 tggcgcagac ggaagctttc acgcgtcaga cgtttaatcc gattgcgttc atgggcgcgt
     4501 cgaacgtttt tcttggcgac tgtaagaccg atacggggat gccccagcga attcaggcgg
     4561 ccgagaatgg taatttgcgg cgtgccagcc cgttgtggct gctggaagac gaatgtgaat
     4621 tgactgggag ttaacaagcg taactccctg ggaaatgcga gcttaaccac tcaggggtta
     4681 gctttattac ttagaaacgg tcagacgagc gcggccttta gcacgacgac gtgccagaac
     4741 ctgacgacca tttttagtag ccatacgagc acggaagccg tgagaacggt tgcgcttcag
     4801 tacagacggt tgaaaagtgc gtttcatggc gatttctacc taaacttgaa taaattcaat
     4861 ggctttattg gatatccgcc gaaaaatgaa acgatggaca ccgaagccat gggtgattaa
     4921 agaggccgga ttgtaataat tgtacactcc ggagtcaatt ctctttcctt atttaccgcg
     4981 cttttccgca ccttttcgca gggaaaacgt acggcctcac accagtgaaa accagcatgg
     5041 cgcgccgggt ggaggattat acgggctgac gggtaaagcg caaggatcgt cctggatctt
     5101 tattagatcg attaagccaa tttttgtcta tggtcattaa attttccaat atgcggcgta
     5161 aatcgtgccc gcctcgcggc aggatcgttt acacttagcg agttctggat catcctgtgg
     5221 ataaatcggg aaaatctgtg agaaacagaa gatctcttgc gcagtttagg ctatgatccg
     5281 cggtcccgat cgttttgcag gatcttgatc gggcatataa ccgcagacag cggttcgtgc
     5341 gtcaccctca agcagggtct tttcgacgta cgtcaacaat catgaatgtt tcagccttag
     5401 tcattatcga cttttgttcg agtggagtcc gccgtgtcac tttcgctttg gcagcagtgt
     5461 cttgcccgat tgcaggatga gttaccagcc acagaattca gtatgtggat acgcccattg
     5521 caggcggaac tgagcgataa cacgctggcc ctgtacgcgc caaaccgttt tgtcctcgat
     5581 tgggtacggg acaagtacct taataatatc aatggactgc taaccagttt ctgcggagcg
     5641 gatgccccac agctgcgttt tgaagtcggc accaaaccgg tgacgcaaac gccacaagcg
     5701 gcagtgacga gcaacgtcgc ggcccctgca caggtggcgc aaacgcagcc gcaacgtgct
     5761 gcgccttcta cgcgctcagg ttgggataac gtcccggccc cggcagaacc gacctatcgt
     5821 tctaacgtaa acgtcaaaca cacgtttgat aacttcgttg aaggtaaatc taaccaactg
     5881 gcgcgcgcgg cggctcgcca ggtggcagat aaccctggcg gtgcctataa cccgttgttc
     5941 ctttatggcg gcacgggtct gggtaaaact cacctgctgc atgcggtggg taacggcatt
     6001 atggcgcgca agccgaatgc caaagtggtt tatatgcact ccgagcgctt tgttcaggac
     6061 atggttaaag ccctgcaaaa caacgcgatc gaagagttta aacgctacta ccgttccgta
     6121 gatgcactgc tgatcgacga tattcagttt tttgctaata aagaacgatc tcaggaagag
     6181 tttttccaca ccttcaacgc cctgctggaa ggtaatcaac agatcattct cacctcggat
     6241 cgctatccga aagagatcaa cggcgttgag gatcgtttga aatcccgctt cggctggggg
     6301 ctgactgtgg cgatcgaacc gccagagtta gaaacccgcg tggcgatcct gatgaaaaag
     6361 gccgacgaaa atgacattcg tttgccaggc gaagtggcgt tctttatcgc caagcgtcta
     6421 cgatctaacg tacgtgagct ggaaggcgcg ctgaaccgcg tcattgccaa cgccaacttt
     6481 accggacggg cgatcactat cgacttcgtg cgtgaggcgc tgcgcgactt gctggcattg
     6541 caggaaaaac tggtcaccat cgacaatatt cagaagacgg tggcggagta ctacaagatc
     6601 aaagtcgcgg atctcctttc caagcgtcga tcccgctcgg tggctcgtcc gcgtcagatg
     6661 gcgatggcgc tggcgaaaga gctgactaac cacagtctgc cagagattgg cgatgcgttt
     6721 ggtggtcgtg accacactac ggtgcttcat gcctgccgga agatcgagca gctgcgtgaa
     6781 gagagccacg atatcaaaga agatttttca aatttaatca gaacattgtc atcgtaaacc
     6841 tatgaaattt accgtagaac gtgagcattt attaaaaccg ctacaacagg tgagcggtcc
     6901 gttaggtggt cgtcctacgc taccgattct cggtaatctg ctgttacagg ttgctgacgg
     6961 tacgttgtcg ctgaccggta ctgatctcga gatggaaatg gtggcacgcg ttgcgctggt
     7021 tcagccacac gagccaggag cgacgaccgt tccggcgcgc aaattctttg atatctgccg
     7081 tggtctgcct gaaggcgcgg aaattgccgt gcagctggaa ggtgagcgga tgctggtgcg
     7141 ctccgggcgt agccgttttt cgctgtctac cctgccagcg gcggatttcc cgaacctcga
     7201 tgactggcag agtgaagtcg aatttaccct gccgcaggcg acgatgaagc gtctgattga
     7261 agcgacccag ttttctatgg cgcatcagga cgttcgctat tacttaaacg gtatgctgtt
     7321 tgaaaccgaa ggtgaagaac tgcgcaccgt ggcgaccgac ggccaccgtc tggcggtctg
     7381 ttcaatgcca attggtcaat ctttgccaag ccattcggtg atcgtgccgc gtaaaggcgt
     7441 gattgaactg atgcgtatgc tcgacggcgg cgataatccg ctgcgtgtgc agattggcag
     7501 caacaatatt cgcgcccacg ttggcgactt tatcttcacc tctaaactgg tggatggtcg
     7561 cttcccggat taccgccgcg ttctgccgaa gaatccggac aaacatctgg aagcaggctg
     7621 cgatctcctc aagcaggcgt ttgctcgcgc ggcaattctc tctaacgaga aattccgcgg
     7681 cgtgcgtctg tatgtcagcg aaaaccagct gaaaatcacc gccaataacc cggaacagga
     7741 agaagcggaa gagatcctcg acgttaccta tagcggtgcg gagatggaaa tcggcttcaa
     7801 cgtcagctat gtgctggatg tcctgaacgc gctgaaatgt gaaaacgtcc gcatgatgct
     7861 gaccgattcg gtttccagtg tgcagattga agatgccgca tcacagtcgg ctgcctatgt
     7921 tgtcatgcca atgagactgt aatgtccctc acccgcttgt tgatccgcga tttccgcaac
     7981 attgaaaccg cggatctcgc cttatctccc ggctttaact ttctggtagg tgccaacggc
     8041 agtggcaaaa ccagcgtgct ggaagccatc tatacgctcg gccatggtcg ggcgtttcgc
     8101 agtttgcaga ttggtcgcgt cattcgccat gagcaggagg cgtttgttct ccacgggcga
     8161 ttacagggcg aagagcgcga gacggcgatt ggcttaacca aagacaaaca gggcgacagc
     8221 aaagtccgca tcgacggtac tgacgggcat aaagtcgcgg aactggcgca cctgatgcca
     8281 atgcagctga taacgccaga agggtttact ttactcaacg gcggccccaa atacagaaga
     8341 gcattcctcg actggggatg ctttcacaac gaacccggat ttttcaccgc ctggagcaat
     8401 ctcaagcgat tgctcaagca gcgcaatgcg gcgctgcgcc aggtgacacg ttacgaacag
     8461 ctacgcccgt gggataaaga actgatcccg ctggcagagc aaatcagcac ctggcgcgcg
     8521 gagtatagcg ccggtatcgc ggccgatatg gctgatacct gtaagcaatt tctccctgag
     8581 ttttctctga ctttctcttt ccagcgcggc tgggagaaag agacagaata tgctgaggtg
     8641 ctggaacgta attttgaacg cgatcgccag ctaacctaca ccgcgcatgg cccgcataaa
     8701 gcggacttac gcattcgcgc cgacggtgcg ccggtggaag ataccttatc gcgtgggcag
     8761 cttaagctgt tgatgtgcgc cttacgtctg gcgcaaggag agttcctcac ccgtgaaagc
     8821 gggcggcggt gtctctacct gatagatgat tttgcctctg agcttgatga tgagcgtcgc
     8881 gggctgcttg ccagccgctt aaaagcgacg caatcgcagg tctttgtcag cgcgatcagt
     8941 gctgaacacg ttatagacat gtcggacgaa aattcgaaga tgtttaccgt ggaaaagggt
     9001 aaaataacgg attaacccaa gtataaatga gcgagaaacg ttgatgtcga attcttatga
     9061 ctcctccagt atcaaagtcc tgaaagggct ggatgcggtg cgtaagcgcc cgggtatgta
     9121 tatcggcgac acggatgacg gcaccggtct gcaccacatg gtattcgagg tggtagataa
     9181 cgctatcgac gaagcgctcg cgggtcactg taaagaaatt atcgtcacca ttcacgccga
     9241 taactctgtc tccgtacagg atgatgggcg cggcattccg accggtattc acccggaaga
     9301 gggcgtatcg gcggcggaag tgatcatgac cgttctgcac gcaggcggta aatttgacga
     9361 taactcctat aaagtgtccg gcggtctgca cggcgttggt gtttcggtag taaacgccct
     9421 gtcgcaaaaa ctggagctgg ttattcagcg cgagggtaaa attcaccgtc agatctacga
     9481 acacggtgta ccgcaggctc cgctggcggt taccggcgag actgaaaaaa ccggcaccat
     9541 ggtgcgtttc tggcccagcc tcgaaacctt caccaatgtg accgagttcg aatatgaaat
     9601 tctggcgaaa cgtctgcgtg agttgtcgtt cctcaactcc ggcgtttcca ttcgtctgcg
     9661 cgacaagcgt gacggcaaag aagaccactt ccactatgaa ggcggcatca aggcgttcgt
     9721 tgaatatctg aacaagaaca aaacgccgat ccacccgaat atcttttact tctccaccga
     9781 aaaagacggt attggcgtcg aagtggcgtt gcagtggaac gatggcttcc aggaaaacat
     9841 ctactgcttt accaacaaca ttccgcagcg tgacggcggt actcacctgg caggcttccg
     9901 tgcggcgatg acccgtaccc tgaatgccta catggacaaa gaaggctaca gcaaaaaagc
     9961 caaagtcagc gccaccggtg acgatgcgcg tgaaggcctg attgcggtcg tttccgtaaa
    10021 agtgccggac ccgaaattct cctcccagac caaagacaaa ctggtttctt ctgaggtgaa
    10081 atcggcggtt gaacagcaga tgaacgaact gctggcggaa tacctgctgg aaaacccaac
    10141 tgatgcaaaa atcgtggttg gcaaaattat cgatgctgcc cgtgcccgtg aagctgcgcg
    10201 tcgcgcgcgt gaaatgaccc gccgtaaagg tgcgctcgac ttagcgggcc tgccgggcaa
    10261 actggcagac tgccaggagc gcgatccggc gctttccgaa ctgtaccttg tggaagggga
    10321 ctccgcgggc ggctctgcga agcaggggcg taaccgcaag aaccaggcga tcctgccgct
    10381 gaaaggtaaa atccttaacg ttgagaaggc gcgcttcgac aagatgctct cttctcagga
    10441 agtggcgacg cttatcaccg cgcttggttg tggtatcggt cgtgacgagt acaacccgga
    10501 caaactgcgt tatcacagca tcatcatcat gaccgatgcg gacgtcgacg gctcgcacat
    10561 tcgtacgctg ctgttgacct tcttctatcg tcagatgccg gagatcgttg aacgcggtca
    10621 cgtctacatc gctcagccgc cgctgtacaa agtgaagaaa ggcaagcagg aacagtacat
    10681 taaagacgac gaagcgatgg atcagtacca gatctctatc gcgctggacg gcgcaacgct
    10741 gcacaccaac gccagcgcac cggcattggc tggcgaagcg ttagagaaac tggtgtctga
    10801 gtacaacgcg acgcagaaaa tgatcaaccg catggagcgt cgttatccga aagcaatgct
    10861 gaaagagctt atctatcagc cgacgctgac ggaagccgac ctctctgatg agcagaccgt
    10921 tacccgctgg gtgaacgcgc tggtcagcga actgaacgac aaagaacagc acggcagcca
    10981 gtggaagttt gatgtccaca ccaatgccga acaaaacctg tttgaaccga ttgttcgcgt
    11041 gcgtacccac ggtgtggata ctgactatcc gctggatcac gagtttatca ccggtggcga
    11101 atatcgtcgt atctgcacgc tgggtgagaa actgcgtggc ttgctggaag aagatgcgtt
    11161 tatcgaacgt ggcgagcgtc gtcagccggt agccagcttt gagcaggcgc tggactggct
    11221 ggtgaaagag tctcgtcgcg gtctctccat ccagcgttat aaaggtctgg gcgagatgaa
    11281 cccggaacag ctgtgggaaa ccaccatgga cccggaaagc cgtcgtatgc tgcgcgttac
    11341 cgttaaagat gcgattgctg ccgaccagtt gttcaccacg ctgatgggcg acgccgttga
    11401 accgcgccgt gcgtttattg aagagaacgc cctgaaagcg gcgaatattg atatttaatg
    11461 gcgttaacca tgcgagcgtg cctgatgcgc tacgcttatc aagcctacga gactcctgca
    11521 atatattgat tttgcatgtt tttgcaggcc ggataaggcg ttcacgccgc atccggcaag
    11581 aagctataag aaaagggcgg agatcatctc cgcccttttt atttctgcaa tccgcaatta
    11641 tcatcccgca gttctatact ttttgggtca acacgtaacg ctttacaggt gattatcatg
    11701 gggctttttg atgaagttgt cggcgccttt ctgaaaggcg atgcggggaa atatcaggcg
    11761 attttaagtt gggttgagga gcagggcggc attcaggtgc tgctggaaaa actgcaaagt
    11821 ggcggcttag gggccattct ctcaacctgg ctgagtaatc aacagggcaa tcaatcggtg
    11881 agcggcgaac aactggaatc ggcgctcggc acaaatgcgg tatccgatct cgggcaaaaa
    11941 cttggcgtgg ataccagtac ggcttccagt ttactggcag aacaattgcc gaagattatt
    12001 gatgctctct cgccacaggg cgatgtagcg ccgcaagcca acaatgattt actttccgca
    12061 gggatggaac tactgaaagg gaaactcttc cgctaagcaa atggggagca tgcaacccgc
    12121 gttgcatccc ccttttccct cgggtgttgt actgattttt gagcggaatc gcgttagcat
    12181 gggtcaggaa ccaatctacc tggggaactc atggctatca aactcattgc tatcgatatg
    12241 gatggcaccc ttctgctgcc cgatcacacc atttcacctg ccgttaaaaa tgcgattgcc
    12301 gcagctcgcg cccgtggcgt gaatgtcgtg ctaacgacgg gtcgccctta tgcaggtgtt
    12361 cacaattacc tgaaagagct gcatatggaa cagccgggtg actactgcat tacttataac
    12421 ggcgcgctgg tacagaaggc cgctgatggt agcaccgtgg cgcaaactgc tctcagctat
    12481 gacgactacc gtttcctgga aaaactctct cgcgaagtcg gttctcattt ccacgcactg
    12541 gaccgcacca cgctgtacac cgccaaccgt gatatcagct actacacggt gcatgaatcc
    12601 tttgttgcca ctattccgct ggtgttctgc gaagcggaga aaatggaccc caatacccag
    12661 ttcctgaaag tgatgatgat tgatgaacct gccatcctcg accaggctat cgcgcgtatt
    12721 ccgcaggaag tgaaagagaa atataccgtg ctgaaaagtg cgccgtactt cctcgaaatc
    12781 ctcgataaac gcgttaacaa aggtacgggt gtgaaatcac tggccgacgt gttaggtatt
    12841 aaaccggaag aaatcatggc gattggcgat caggaaaacg acattgcaat gattgaatat
    12901 gcaggcgtcg gtgtggcgat ggataacgct attccttcgg tgaaagaagt ggcgaacttt
    12961 gtcaccaaat ctaaccttga agatggcgtg gcgtttgcta ttgagaagta tgtgctgaat
    13021 taatcagtgg acgggcaaac agcataaacg cgtttgcccg cttactgatt attggtttac
    13081 tgcatataca accagaaatt gcagcggtgc agtaatgatg tccagcgtaa cagtcactgg
    13141 cagtaatgct gcggaaacac catccggaat gcctctgggt gaatagtatt cataaaatcg
    13201 cactttaaat ggatggtaga acgccatcac ctttgagtaa tccatgtttt tatttttctc
    13261 atggattgtg ccttttaggt tttccagtga cctggtacag tgttgaacac cacaggcaaa
    13321 accataatgc gttgccagtg ccttttcttc ttcgttattc cagtagtagt aaagcgatat
    13381 tgtgccggta aatttctttt tccccgcatt taaaacaaat cttgcgtcat cagcaacctg
    13441 aatattgtgc cggttaagtg ctggatcatt gagcatctta acgatgtcat cagcaccatc
    13501 tgtgataagg taatccagcg attcccctac aaaaacatag ccttttgttc cattactatc
    13561 ctttgcctgc gatagccccg tgatggtgtc tgactgcgat tgatactttc ctgaagtacc
    13621 ctttgcggta tgggaaacca gagcatacga gatacagcct gaaagcatca gtgctagtgg
    13681 gaataaactg gcagccttaa aaaatccctt aaaattcaac ttcatgatta atcctttaaa
    13741 cgagtagcta agccgttgaa agcatactct tctttccctc ctgtttagcg cctgatctcc
    13801 cttttcagat ttctgcccga tataagacct tttcttatga ggtgtttttt tgtggactaa
    13861 tatctgttgc aaggctatgg cttcgccccg gcactggttt atatctccac cgtcgcgttg
    13921 attggtgcgc tctcttacat cctgctggtg ggcgatgtga agcgcgttgg ctaatccttc
    13981 aactgtggaa tatgtgccac accactgcgc ataatccact ttcttaaagc cggtgcggtc
    14041 aggcatcggc tttttactat cttgccgaag agttttaaac gcagtttgcg ggtggcgcgc
    14101 cagtaggctg cgttgatctt ctctggctgc ttcagtaaca ccgaacgcag aatctctgcg
    14161 ctatccagcg catagcttat cccctccagc gagctggcgc tgataaatcc cgccgcttca
    14221 ccaatcagaa aggcgttgtc cttaccgcag ataaaatcct gccagcgtga gggaaacagc
    14281 actgtgcatt tttcgctttt caccgctttg ccaaactgga actgaaaggc gctcattttc
    14341 tctttcagcg tcgcgaaacg cgtctgaccg tctttcattg gataggcacc accaaagata
    14401 aaataaccgt ctttgctgat actccatgag taacagtcag ttatcgcatt atcaaagatg
    14461 caggagtaga acggcaccgg atgtttctct gcgaaccact gctggattgc gacatattta
    14521 cggatttgat gatccgggta gagatgtcgc cgcaccatcg agttggcacc atcggcaccg
    14581 accagatagc gggcggtaat atgctgctcc cagccgtctg cacgaaaaat gacatgccat
    14641 ttattatcct cacgccagat tttccggcac aggctatcgt ggtaaacctc aacgctggcg
    14701 gggatcagcg atttcatcca caagtcgaaa gcatggcgat taatattgat atagcttcgc
    14761 tggtagttac gcgtgagcga tgcggcgacg tcgacggttt tgacgctgaa aatctgcgga
    14821 ttagcgatca catcgacagg aagcgtcagt ccatcgcgaa taaaagatcg ctgcgcgtcc
    14881 ggtgccagca gaccgccgca gggtttgctg aaaccttcag taccacactg gtgcttttta
    14941 tccagcgcga tcacctgcat tttgcctgct aactttcgtg ccaacgccga tcctgccggg
    15001 ccgaggccaa taatcgccac gtcgaaatgt tccatctttt cactcacatt catcacgatg
    15061 gcaaaagact attgtctgaa agtgaagtcc ttgtgaagta tgatggcgtg aaccgttttg
    15121 tatcaggacc acgccatgaa acagataacc tttgctcccc gtaatcacct gctcaccaat
    15181 accaatacct ggacgcccga cagccagtgg ctggtatttg acgtgcgtcc ttcaggggcg
    15241 tcgtttaccg gcgagaccat tgagcgtgtg aatatccata ctggagaggt cgaggttatc
    15301 tatcgcgcgt cacagggcgc acacgtcggc gtggtgaccg ttcatccgaa gtcagaaaaa
    15361 tatgtcttca ttcacggccc ggaaaatcct gatgaaacat ggtattacga tttccatcat
    15421 cggcgcggag tcattgctga aggcggcaag gtgagcaatc tcgatgcaat ggatattact
    15481 gcaccgtaca ccccaggagc gctgcgcggc ggcagccatg tgcatgtctt tagcccgaac
    15541 ggtgaaatgg tgagctttac ctataacgac catgtaatgc atgaacgcag cccggcgctg
    15601 gatttgcgaa acgtcggggt tgctgcaccg tttggcccgg tcaacgtaca aaagcagcat
    15661 ccgcgtgaat acagcggtag ccactggtgc gcactggtga gtaaaacaac gcccacgccg
    15721 cagcctggca gcgatgaaat caatcgtgct tatgaagaag gatgggtagg aaatcacgcg
    15781 ctggcgttta ttggcgacac actttcgcca aagggcgaga aagtaccgga gctgtttatt
    15841 gtcgagttgc cgaaagatga agctggctgg aaagcagctg gtgatgcgcc gttaagcgga
    15901 acggaaacaa ccctgcccgc gccaccgcgt ggcgtcgtgc agcgacgttt aacctttacc
    15961 caccatcggg cttatccggg gttagtcaac gtcccgcgcc actgggtgcg ctgtaatccg
    16021 cagggtacac aaatcgcgtt tttaatgcga gatgataacg gcattgtgca actgtggctt
    16081 atctcgccac agggcggcga gccgcgccag ttaacccata acaaaacgga tattcagtct
    16141 gcatttaact ggcatccatc aggagaatgg ttgggctttg tgctggataa tcgaattgct
    16201 tgtgcccatg cggaaagcgg ggaggttgag tatttaaccg aaaaccacgc caatccacct
    16261 tctgcggacg ccgtggtctt ctcgccggat ggtcaatggc tggcgtggat ggaaggcggt
    16321 cagctgtgga tcaccgaaac tgatcgctaa atcagttatc aggcattctg gccggtggaa
    16381 taaccgcatt ggtggcttgc gcgttggctt cgcttttttc aacgcgtgag cgcaccgagc
    16441 tgtctttgcg gaacacatcc cacggcagta aaagtgtgtc catcacagcg gtaaacggca
    16501 tatcgagaat cgccagcgac ttagtgcccc agttggtttc atcgtcgcca atcatgctgg
    16561 cgctggcgcg ggtgccggga tacgttcctt ctttcccccc ggtatgggac atcacgctgg
    16621 agcatcctcc gagtaatgtc attccgctgc atatcatgaa cgctaacaac acatttctta
    16681 tcataataaa ttcatctgtt gatcgtgggt gttggcctga tgagttatag cgatcccttg
    16741 ctgaaaataa catcatcatt acgtcgcact gtggcggcta tcgcacttta acgtttcgtg
    16801 ctgccccctc agtctatgca atagaccata aactgcaaaa aaaagtccgc tgataaggct
    16861 tgaaaagttc atttccagac ccatttttac atcgtagccg atgaggacgc gcctgatggg
    16921 tgttctggct acctgacctg tccattgtgg aaggtcttac attctcgctg atttcaggag
    16981 ctattgatta tgcgtaactt tgatttatcc ccgctttacc gttctgctat tggatttgac
    17041 cgtttgttta accacttaga aaacaaccag agccagagta atggcggcta ccctccgtat
    17101 aacgttgaac tggtagacga aaaccattac cgcattgcta tcgctgtggc tggttttgct
    17161 gagagcgaac tggaaattac cgcccaggat aatctgctgg tggtgaaagg tgctcacgcc
    17221 gacgaacaaa aagagcgcac ctatctgtac cagggcatcg ctgaacgcaa ctttgaacgc
    17281 aaattccagt tagctgagaa cattcatgtt cgtggcgcta acctggtaaa tggtttgctg
    17341 tatatcgatc tcgaacgcgt gattccggaa gcgaaaaaac cgcgccgtat cgaaatcaac
    17401 taattcccta aggccgcctt gcgcggcctg acatctccat gctcgccgtc agggagcata
    17461 tgcgaatctt cggatttgca ggtacttact cgcttcttag aaggagaaat gactatgcgt
    17521 aacttcgatt tatccccact gatgcgtcaa tggatcggtt ttgacaaact ggccaacgca
    17581 ctgcaaaacg ccggtgaaag ccagagcttc ccgccgtaca acattgagaa aagcgacgat
    17641 aaccactacc gcattaccct tgcgctggca ggtttccgtc aggaagattt agagattcaa
    17701 ctggaaggta cgcgcctgag cgtaaaaggc acgccggagc agccaaaaga agagaaaaaa
    17761 tggctgcatc aagggcttat gaatcagcca tttagcctga gctttacgct ggctgaaaat
    17821 atggaagtct ctggcgcaac cttcgtaaac ggtttactgc atattgattt aattcgtaat
    17881 gagcctgaac ccatcgcagc gcagcgtatc gctatcagcg aacgtcccgc gttaaatagc
    17941 taactagcta ttctctttgc cccgccattc tggtggggct ttttttgtct ttcttcttct
    18001 acaatgcgtt aaatttcgac tgtttaagat atttcggcac gttctttccc gtgataatgt
    18061 gcgctataag gcaaatctgc ttcaccgatt cagccataat ccgtagcaat taaaatgtta
    18121 tctcaagaga aatggacgat gagtgatata gcattaacgg tcagtattct ggctttggtg
    18181 gcagtcgtcg gtttgtttat cggcaacgtc aaatttcgcg gcataggatt aggtattggc
    18241 ggcgtgctgt ttggtgggat catcgtcggc cattttgttt ctcaggcggg gatgacatta
    18301 agtagcgata tgctgcatgt tattcaggaa tttggtctga tcctgttcgt ttatactatc
    18361 gggattcagg tagggccggg cttctttgcc tcattgcgcg tctccggatt acgcctcaac
    18421 ctgtttgctg ttctgatcgt catcatcggt ggtctggtta ccgccatcct gcataaactg
    18481 tttgatattc cactgccggt agtgctgggg attttctccg gtgcggtaac caatacgcca
    18541 gcgctggggg cagggcagca gatcttgcgc gacctgggta caccaatgga aatggtcgat
    18601 cagatgggga tgagttacgc gatggcgtat ccattcggca tttgcgggat attgttcacc
    18661 atgtggatgt tgcgggttat tttccgcgtc aatgtcgaga cagaagccca gcagcacgaa
    18721 tcttcacgca ccaatggcgg cgcgctgatc aggactatca atattcgcgt tgagaaccct
    18781 aacctgcatg atttagccat taaagatgta ccgattctca acggcgacaa aattatctgc
    18841 tcgcgtctga aacgcgaaga aaccctaaaa gttccttcgc cagataccat tatccaactg
    18901 ggcgatttgc tgcatctggt gggtcagcca gcggatttac ataatgcgca actggtgatt
    18961 ggtcaggagg tcgatacttc gctgtccacg aaaggcactg atttgcgcgt cgagcgtgtg
    19021 gtggtcacca atgaaaacgt gcttggtaag cgtattcgcg acctgcattt taaagaacgc
    19081 tatgacgttg ttatctcgcg cctgaaccgt gccggggtcg aactggtcgc cagtggcgat
    19141 atcagcctgc agttcggcga tatcctcaac ctggtggggc gtccgtccgc aattgatgcc
    19201 gttgccaatg tgctggggaa tgcgcagcaa aaactgcaac aggttcagat gctgccagtg
    19261 tttattggca tcgggcttgg cgtattgtta ggttctattc ccgtctttgt gcctgggttc
    19321 ccggccgcgt tgaaactggg gctggcgggc ggtccgctga ttatggcgtt gatcctcggg
    19381 cgtatcggca gtatcggcaa gctgtactgg tttatgccgc caagtgccaa cctcgcgctg
    19441 cgggagctgg ggatcgtgct gttcctctcg gtcgttggtc tgaaatctgg tggggatttt
    19501 gtgaatacct tggtcaatgg cgaagggcta agctggattg gttatggtgc cctgatcacc
    19561 gccgttccgc tgattactgt tggcattctg gcgcggatgt tagccaaaat gaattacctg
    19621 accatgtgcg ggatgctggc aggttccatg accgatcctc cggcgctggc gtttgctaat
    19681 aatcttcatc caaccagcgg tgcggcggcg ctctcttacg ccactgtcta tccgttagtg
    19741 atgttcctgc gcattatcac cccccaatta ctggcggtgc tcttctggag tatcggttaa
    19801 ctcttgtccg ggtggagtcg ccgtaaatgg tactccacct ggtattcgct ggcatttctg
    19861 aacatcactg aataattcaa aaactccccg ctctcgctat atgacaacga ggtgatccgc
    19921 agcagtggcg tgtgttccgc taccttcatt tgccgcgcca gcagtctatc ggcgagcacc
    19981 ggcgagcacc ggcgtcaggc tttcataatt accgccaatc agaatcccgc actcttgttc
    20041 aatatattca aacttcgacc cttccagatg ttgcagcgaa agattacgga acagttttac
    20101 cggcatatag ctgtcttcca gcatcagcgg tttcccttcc acaaaacgaa cgcgacggga
    20161 gaagtagatc tgctcgttga tttgaatccg taactggctg gcaattgccg gaggcgcagg
    20221 catgatttca aaaatcagca cctggctggt gactgttttt ccctgccgtt ttaacacctc
    20281 caccagcccg gtcaggctgg cggtttgatg cagtacatct ttgcgcacca ggtaagtgcc
    20341 gctgccgtgg cggcggacca ccagccccca cgctaccagc aggtcaatgg ctttacggat
    20401 ggtcatccgc gataccgcaa actcttccgc cagctttttt tcaccgggaa gaaggctgtt
    20461 tagcgtgaaa tctgcggaat taagtctaat tcttaaccgc tccgcaatgc ttttgtagat
    20521 catctctaga tctctttttt gcctgcaagc ccgggtaatg cgcccacaag ttctgcttaa
    20581 tcgattgaaa aaacatcttt tttttaaaga tgtgttcgat gctgtgtacc tgtgctcaca
    20641 gaggtctact ttttcgcgaa aacgtagatc tctaccgccc aacgaaaagc atgaaagcga
    20701 tcacgaatcc cattgggtcg tcatgttctg ccgatcgcat cttcctatcc tcgctccagg
    20761 cctgccgcat aaccaatcag gcttcctact tacagaattg agaaaagagg atgtggaaat
    20821 gctcagtcaa attcaacgct ttggcggcgc gatgttcacg ccagtgctgc tgtttccctt
    20881 cgccgggatt gtggtgggtc ttgccatctt gctgcaaaac ccgatgtttg tcggggaatc
    20941 actgaccgat ccgaacagtt tattcgcgca aatcgtacac attattgaag agggcggatg
    21001 gacggtattc cgtaatatgc cgctgatttt tgctgtcggt ttacccattg gccttgctaa
    21061 gcaagcgcag gggcgtgctt gtctggcggt gatggtgagt ttcctgacct ggaactattt
    21121 catcaacgcg atgggaatga cctggggaag ctacttcggc gtcgatttca ctcaggacgc
    21181 ggtggcaggt agcggtctga caatgatggc cgggattaaa accctcgata ccagcattat
    21241 cggcgcaatt atcatttccg gcattgtgac ggcgctgcat aaccgtctgt tcgataaaaa
    21301 actgccggtg tttctcggca ttttccaggg gacgtcttat gtggtgatta tcgccttcct
    21361 ggtgatgatc ccctgtgcct ggctgacatt gctcggctgg ccaaaagtac aaatggggat
    21421 tgaatctctg caagcgttcc tgcgttcggc gggtgcgctt ggggtgtggg tttacacctt
    21481 cctcgaacgt attctgatcc caaccggttt acaccacttc atctacggac cgtttatctt
    21541 tggtccggca gctgttgaag gcggcattca gatgtactgg gcgcagcatc tgcaagagtt
    21601 cagtttgagc gccgagccgc tgaaatcgtt gttcccggaa ggcggttttg ccctgcacgg
    21661 taactcaaaa atctttggtg ccgtgggcat ttctttagcg atgtacttca ctgccgcacc
    21721 ggaaaatcgg gtaaaagtgg cgggcttgct gattcccgca accttaaccg ccatgctggt
    21781 gggaattacc gaaccgctgg aatttacctt cctgttcatt tcaccgttgc tgtttgcggt
    21841 acacgctgtg cttgcggcct caatgtcgac cgtgatgtat ctctttggtg tggtgggcaa
    21901 catgggcgga ggtctgattg accaggtttt accgcaaaac tggatcccga tgttcagcaa
    21961 ccacgcggat atgatgttga cccaaatcgc cattgggttg tgctttaccc tgctgtactt
    22021 cgtggttttc cgcaccctga ttctgcaatt caacatgtgc acgccgggac gtgaagatgc
    22081 ggaagtgaaa ctctactcaa aagccgaata caaagcctcg cgaggccaaa ccaccgcggc
    22141 agagccaaaa aaagagctgg atcaggctgc cggtatcctg caagccctgg gcggggtcgg
    22201 caatatctcc agcatcaaca attgcgcgac gcgtctacgt attgcactgc atgacatgtc
    22261 acaaacgctg gatgacgaag tctttaaaaa gctgggagcg cacggcgtct tccgtagtgg
    22321 cgatgccatt caggtgatca ttggtctgca tgtatcccag ctgcgtgaac agctcgatag
    22381 cttaattaat tctcatcaat cagcagaaaa tgttgccatt acggaggcag tataatgacc
    22441 aaattctcag tggttgtcgc aggcggtgga agcaccttta cgccaggcat cgtgttgatg
    22501 ctcctggcga atcaggaccg tttcccgctt cgtgcactga aattttatga taacgatggc
    22561 gcgcggcagg aagtgattgc cgaagcctgt aaagtcatcc ttaaagagaa agcgccggac
    22621 attgcgttta gttacaccac cgatcctgaa gtggcgttca gcgacgttga ttttgtcatg
    22681 gcgcacatcc gcgtcggcaa atacccgatg cgcgaactgg atgaaaaaat cccgctgcgc
    22741 cacggcgttg ttggtcagga aacttgcgga cccggcggaa tagcgtacgg catgcgttcc
    22801 attggcggcg tcctggaact ggtggattat atggaaaaat attcaccaaa cgcctggatg
    22861 ctcaactact ccaacccggc agccattgtc gcagaagcca cgcgtcgtct gcgcccgaat
    22921 gcgaaaatcc tcaacatctg tgacatgcca atcggtattg aaagccggat ggcgcaaatt
    22981 gttgggctgc aagatcgcaa acagatgcgc gtgcgctact acggcctgaa ccactttggc
    23041 tggtggacat caattgaaga tttgcagggc aacgacctga tgccccagct gcggcaatat
    23101 gtctcgaagc acggttatgt tccaccgcag caagatacac atactgaagc gagctggaac
    23161 gacacctatg caaaagcgcg ggatgtccag gcactggacc cggatacatt accaaacacc
    23221 tatctgaaat attatctctt cccggattat gtcgttcagc attccaaccc tgaacatacc
    23281 cgcgcgaatg aggtgatgga acatcgcgag aaacaggttt tcgatgcttg ccgcgccatt
    23341 acggcggcag gaaattcagc ggcgggcaag ctggagattg acgaacatgc gtcatacatc
    23401 gtcgatctgg cggcggcaat tgccttcaac actcaggagc ggatgttgct gattgtgcct
    23461 aacaacgggg caatccataa ctttgatgat gaagcgatgg tcgagatccc gtgtctggtc
    23521 gggcacaacg gaccagaacc actggtggtc ggcgatatcc cgcagtttca gaaagggtta
    23581 atgagtcagc aagtggcggt ggaaaaactg gtcgtggacg cctgggaaca gcgttcatat
    23641 cagcacctgt ggcaggcgat tacgttgtcg aaaacggtac cgagcgcctc ggtcgccaaa
    23701 gctattctgg atgagttgct ggaggccaac aaagcgtact ggccagagtt acgttaattg
    23761 tgtggactgg catccgttgg gtgctggtcc gtattcttta gcacggctga aggcgcataa
    23821 ccaaatttat gtttaaaggc tttgctgaaa tgaaaggagt cgaagaagtt gagcgtatca
    23881 gcgacttcgc caaccgagtt tccttgttgc tttaatagcg atagcgccag atccagacgc
    23941 gcatcgaggt aatattcttt cggcgtcttg ccggtatagc gtaaaaacag acgacgcaac
    24001 caggcttcgc tacaggggat cgtggcagcc atatcggcta cgctccagcg ttgttgcaga
    24061 ctggcatgta aagtggcaat taatttttca atttgccgcc gttgtggatc ttttttaccg
    24121 tctgcgtaca taagacaaat ccactgataa ataattttcg tcagaaaagc gactgccaga
    24181 ttatttttta ttgcttctgg tgaagttatt aactcagcaa cttcggtgag ttcctgatta
    24241 taaatttcgc cattataaat aacgctttgc tgaccaacgg gaatatccat catactggtg
    24301 ggggtaaatt ccatccagta ctgttcccag actaaacctt cacagtgata agagtgaata
    24361 tccattggct ttaaaaatat aatacagttg ccatgcagag ttatttgttc gccattcttt
    24421 aaaagaattt tcccacatcc ttgtacggta tacaccgcaa cccatgagtt ggcgataagc
    24481 ggtttcttct tgtcccgcgg ccagatcacc cggtaatgct cgtcggcgta ggtatgccag
    24541 agcgagatca gagaaattcc gctggagatc acattttcgt taatcaataa gggaatattg
    24601 tatggagttt cgttttttac atctgaagtt tgcaattttc cattcatttt aatatccctc
    24661 cggcgaatta tgctcacaac ctgaacttaa cagaaaacaa tccgttcagg gatacgacgt
    24721 ttgtatttta agaatctgac tgcctgaccc gacgcatttt acctctccct atattcatgc
    24781 gtccgggaca taaacataaa taagggctat gagatgaatt cgttacaaat cttgagtttt
    24841 gtcggtttta cgctgctggt ggcgatcatc acctggtgga aggttcgcaa aacagatacc
    24901 ggatcgcaac aaggctattt tcttgccgga cgttcactaa aagcgccggt tattgctgct
    24961 tcgttaatgt taaccaacct ttccacggaa caactggttg gtctttccgg gcaggcctac
    25021 aaaagcggca tgtcggtgat gggctgggaa gtgacttcag cggtgacgct gatcttcctc
    25081 gcgctaatct ttttaccgcg ctatctgaag cgcggcattg ccaccatccc cgattttctg
    25141 gaggaacgtt atgataaaac gacgcgtatt atcatcgact tctgcttcct cattgccacc
    25201 ggcgtctgct ttctgccgat tgttctctac tccggcgcgt tggcgctcaa cagcctgttt
    25261 cacgtcgggg aatcgctaca gatttctcac ggtgcggcta tctggctact ggtaattttg
    25321 cttggtctgg cgggaatttt gtatgcggtg atcggcggac tgcgcgcaat ggcagtggcg
    25381 gactccatca acggtattgg gctggttatc ggcgggttga tggtgccggt atttggcctg
    25441 atcgcgatgg gcaagggcag ctttatgcag ggcattgagc aaattaccac cgttcacgcc
    25501 gagaaattaa actcagtcgg tggcccgacc gatcccttgc cgattggcgc ggcatttacc
    25561 ggtttaattc tggtgaacac cttttactgg tgtacaaatc agggcatcgt gcaacgcacg
    25621 ctggcgtcaa aaagcctggc ggaagggcaa aagggggcgc tgttaacggc ggtgctgaaa
    25681 atgctcgacc cgctggtact ggtgctgcca gggttgattg cgtttcatct gtatcaggat
    25741 ctaccgaaag ccgacatggc ctacccgacg ctggtcaata acgttctgcc agtgccactg
    25801 gtgggtttct tcggcgcggt gttatttggt gcggtgatca gtaccttcaa cggctttctg
    25861 aatagcgcca gtacgttatt cagtatgggt atttaccgtc gcatcattaa ccagaatgcc
    25921 gagccgcagc agctggtcac cgtcgggcgc aaatttggtt tctttatcgc tatcgtttcg
    25981 gtgctggtag cgccgtggat cgccaacgcg ccgcaggggc tgtatagctg gatgaaacag
    26041 ctcaacggca tttacaacgt gccgctggtt accatcatca ttatgggctt tttcttcccg
    26101 cgcatcccgg cgctggcggc aaaagtggcg atggggattg gcataatcag ctacatcacc
    26161 atcaactatc tggtgaagtt cgacttccat ttcctctatg tgctggcctg tacgttctgc
    26221 atcaacgtgg tcgtgatgct ggtgatcggt tttatcaaac cgcgcgccac gccgttcacc
    26281 ttcaaagatg cgtttgcggt ggacatgaaa ccgtggaaaa acgtcaagat cgcgtcaatt
    26341 ggcatcctgt tcgcgatgat tggcgtctat gccgggctgg ctgaattcgg cggctacggt
    26401 acgcgctggt tagcgatgat cagttatttc attgccgccg tagtgattgt ctacctgatt
    26461 tttgacagct ggcggcatcg tcacgaccca gccgtaacct ttactcccga tgcaaaggat
    26521 agcctatgaa acgccccaat tttctgttca tcatgaccga tacccaggcc actaatatgg
    26581 tcggttgcta tagcggtaag ccgctgaata cgcaaaatat tgatagtctg gcggcggaag
    26641 gtattcgctt taactccgcc tacacctgtt caccggtttg tacaccggcg cgcgccgggc
    26701 tgtttaccgg tatctacgct aaccagtccg gcccgtggac caacaacgtc gcgccaggca
    26761 aaaacatttc cactatgggg cgctacttta aggatgcggg ctatcacact tgttacatcg
    26821 gcaaatggca tctcgacggt catgactatt tcggcactgg cgagtgtccg ccggagtggg
    26881 acgctgatta ctggttcgat ggggcgaact atcttagcga actgacggaa aaagagatta
    26941 gcctgtggcg caatggccta aacagcgtcg aagatttaca ggcgaaccat attgacgaaa
    27001 ccttcacctg ggcgcaccgc atcagcaatc gggcggtgga ttttctgcaa cagcccgcgc
    27061 gcaccgagga acccttcctg atggtggttt cgtatgatga gccgcatcac ccgttcacct
    27121 gtccggtgga gtatttagag aaatacgctg atttttacta ccaactgggc gagaaagcac
    27181 aggatgacct ggcgaacaaa ccagaacatc accgcttatg ggcgcaggcg atgccatcgc
    27241 cagtcggtga tgacgggctt tatcaccatc cgctctattt tgcctgtaat gactttgttg
    27301 atgaccaaat aggacgggtc atcaatgcct taacgccaga gcaacgtgaa aatacgtggg
    27361 ttatttatac atccgatcac ggcgaaatga tgggcgcaca taagctgatc agtaaaggag
    27421 cggcgatgta tgacgacatc acccgtattc cgctgatcat ccgttcgccg caaggggagc
    27481 ggcgacaggt caatacgcca gtcagtcata tcgatttact gccgacaatg atggcgctgg
    27541 cagatattga aaaaccagag attctgccgg gggaaaatat ccttgccgtg aaagagccac
    27601 gcggcgtgat ggtggaattt aaccgctacg agattgagca tgacagcttt ggcggtttta
    27661 ttccggtgcg ttgctgggtg acggatgact ttaaactggt gctcaacctc ttcaccagtg
    27721 atgaacttta cgatcgccgt aatgacccaa atgaaatgca taacctgatc gatgatgccc
    27781 attttgccga cgttcgcagc aaaatgcatg atgccttatt ggattatatg gacaaaattc
    27841 gcgatccgtt ccgcagctac caatggagcc tgcgtccgtg gcgtaaagat gcacggccgc
    27901 gctggatggg ggcgtttcgt ccacgcccac aagatggcta ttcgccagtg gtacgtgact
    27961 atgacaccgg cctgccgaca caaggggtga aagtggagga taaaaaacag aagttctgac
    28021 gataatgccc gctgatgatc acccggcggg cattattcag gctgcttaaa ccagtgaacc
    28081 gccggtcagg aaggaaaaga taaggaatat cagcaaaatg ctgcacacga tgccgatacc
    28141 aaaaagcaag gtatggaaag ccagcgtccc gccgccaaaa accagcgcgc cgcgaatgcc
    28201 ccagcgattt tccttacgca taccgccaat aaatgcgcaa agtagcgcag caatggccag
    28261 tgcgataccg ctttgatcga ggattttatc gacatctacg tcctcttttt tctccacggt
    28321 ggtaattttt tcgcctttta ttccggcgag aagacctctt ttaatttcag ccgttttgtc
    28381 cgccacgatg ctttcaagcg ttggcggtgg tgcggagaaa gggccgaatg aaaagtggat
    28441 aatccccatc attaaagcga tggcaccgaa tagcatgccc aatgaactga ttttattttg
    28501 cgctatgata cccatctgac ctccttgtag cggatggcaa aagcatatca ggaaacgggc
    28561 ggacgaaatt cttaaaaaaa accgctaaag gtagagcgtt agtgaaattg caaaggagca
    28621 acaatgaaaa tttcccgcct cggagaagcg cccgattacc gcttctcgct ggctaatgag
    28681 cgtacttttc tggcgtggat ccgtactgca ctaggatttc tggcggcggg ggttgggctt
    28741 gatcaacttg cgccagattt cgccacgccc gtcattcgcg aactgctggc gttgttgttg
    28801 tgtctgtttt ccggcgggtt agcgatgtat ggctacttgc gctggttacg caatgaaaaa
    28861 gcgatgcgtc tgaaagagga cctgccctac accaacagct tattaatcat cagcttaata
    28921 ttgatggtcg tagcagtgat cgttatggga ctggtgttgt atgccggata gccgcaaagc
    28981 cagacgcatt gccgacccag ggctgcagcc ggagcgtaca tcattggcgt ggtttcgtac
    29041 catgctgggc tacggcgcgt tgatggcgtt ggctatcaaa cacaactggc accaggcggg
    29101 catgttattc tggatttcga ttggcatcct cgccatcgtg gcgctgatcc tctggcacta
    29161 cactcgtaat cgcaatttaa tggatgtcac gaatagcgat ttttcccaat ttcacgtagt
    29221 ccgtgacaaa tttttgatct ccctcgcggt gttatctctc gcaatactgt ttgctgtaac
    29281 gcatatacat caacttatcg tatttattga gagagtcgca tgacaggaag tcaggtcatt
    29341 gatgctgaag aggacaggca taaactggtt gtagagtata aggatactct gcaacctgcc
    29401 gatttttatc acaatttcaa acagcggggc atccgctccg tacagcttat tccccatctt
    29461 gaatttgatg accggggcga tatgacggcc gcttcggtga cagcagagct gtgggggaaa
    29521 tttttaattg ccctgtttga gtgttgggtg cgggcagata tcagtcgtat ttcgattgag
    29581 ctttttgacg ccactttaca aaaatggtgt ggcagcgaaa acccgcaccc gcgccgtgac
    29641 tgccaggcgt gcgactggca tcggctgtgt ccgcacgctc gggaggagac gccagacaac
    29701 gtgctttgtg cgggctatca ggcgttttat tcttactccg caccgcatat gcgggtgatg
    29761 cgtgatctga taaagcagca tcgttctccc atggagctga tgacgatgct gcggtgatgt
    29821 gcgcttaaac gggctgcccc tgatgcgaca tccgcgtcgc cagcggtagc cagcacagca
    29881 cgatcaacaa tcccattaag gtcatcaaca accccaggct gccctgaccg gtttgcggca
    29941 acatcgctga gagcgacgcc agcacgccgg aaccaatgtt ttgcagacca ccgaccagcg
    30001 cgccagccgt gcccgccagg aaggggaacg gctccatcgc gccgctggtc gccagcggaa
    30061 acagcatccc ggcaccgaaa aagaatagcg cggcgggaac aagcagcgtc cagacattca
    30121 tcacgccaaa ccagtcgggg atccacatca gcaagccagc cagcaggcag cagataaccg
    30181 actgccacat caacgttgag aagcgtttat taggacgccc ggcaaaccat gccccaaaaa
    30241 atgccgccgg aatcggcaga ataaacaaaa tactgaccgt catactgctc agccctaaca
    30301 ccgcgcccat cagcacgccg gagcaggctt caaaggcggc aatcccggcc agaccgccaa
    30361 tcagcatcag caaataacag ttaaaaccgc cgttaccgaa aagcgtttta taactggtaa
    30421 gcaggcgcgt gcgcggtgca tcgaccggac gcgtttccgg catccagcgg gccatactga
    30481 aggtcacgcc agcacaaaga accaacaaaa agagataaca ggcgcgccag ttccacatcg
    30541 tatccagcag accgccgatt agcggtgcga gcaacggact aacaagaatc cccatgttta
    30601 acaggctgtt agcatggcgc aactgtgtcc gttcatataa atcacgcggc aaagtacgcg
    30661 ccattacgcc gccaacgccg gtgcccatcc cctgcatcgc gctggcggca atcaataccg
    30721 tcaaactgga ggtcgtgacc gcgaccagcg ttgccagcat aaaaatggac attccgacga
    30781 ggatcaccgg tcggcgaccc acacggtcgg aaatcgggcc ataaaacagc tgtgagacac
    30841 cgtaagtcag cagataagcg cccattacgc tctgcaccgc cccttcacgg acgttgagat
    30901 cgcgcgccat atcggcaata gctggaatat aaatggtttg cgccatctga ccgacggcca
    30961 cgagtaatac caacatcaat aacaaattga cgtttctatg ccttttcatt atcacggatg
    31021 cttttataaa aataattaga ataagtcact acgcattccc ataaatgcgg gagaggaatc
    31081 tatcacattg atgaatgtta gccagatatg tgccaggaat ggcgaaaaga aaaacggcag
    31141 aatatgtaaa caaaagcggc aataaatgtt gccggaaggg atggtaagtc ggataagatg
    31201 cgtcagcatc gcatccgaca ccaacccaca agcagaatat aaaaggggag cggtttcccg
    31261 ctcccctttg gtgcgacttg aatctgaatt acttcaggta tttcagaaca gcatcaagta
    31321 gttgcagtgc tgcaacaatg agtttgagga taagaatggc gatatccacc aggctcatac
    31381 gcgtctcctg tggttcagga gcactgccag ctgacgtacc tttccgctgc ctgttgccag
    31441 ctgttgtgca cgcggtatct caaggagaaa cgttgtgctt gttgacataa cacagtgtgc
    31501 tcacgcgggg ttaaggcgct gacggcacca cccgtttcag ctaggactta gtgcgccggg
    31561 taacgatgcg gcccccgcat aacacattgc gtacagtgat attatagaag tttactgtat
    31621 aaataaacag taatagttgg acaaaacgca aactgtgatc gatactcatc tatgcactaa
    31681 atacgtcaaa attcgtgccg aaattgcgcg ttctgcgcgg aacaggtata ctttcagtgt
    31741 tgacataata cagtgtgctt tgcggttacc agccgcaggc agctgacgaa acctcgctcc
    31801 ggcggggttt tttgttacct gcaattcagt ataaaacgtg atcaacccct caattttccc
    31861 ttgctgaaaa attttccatt gtctcccctg taaagctgtg cttgtataaa tattgttaaa
    31921 cacaaaacca acaaggtccc caatgactac ttccatgttc aacgcaaagc tactaccaac
    31981 tgcgccatcc gccgcagtgg tcgtcgtgcg tgtggtggtg gtcgtcggca atgcgccgta
    32041 gggactggaa caacacacga ttccaaaacc ccgccggcgc aaaccgggcg gggtttttcg
    32101 tttaagcacc tcccggaaag tcggcccaga aaaaaaggac tggagcatgg caagttcggg
    32161 cacaacatcg acgcgtaagc gctttaccgg cgcagaattt atcgttcatt tcctggaaca
    32221 gcagggcatt aagattgtga cgggcattcc gggcggttct atcctgcctg tttacgatgc
    32281 cttaagccaa agcacgcaaa tccgccatat tctggctcgc catgaacagg gcgcggggtt
    32341 tatcgctcag ggaatggcgc gcaccgacgg taaaccggcg gtctgtatgg cctgtagcgg
    32401 gccgggtgcg actaacctgg tgaccgccat tgccgatgcg cggctggact ccatcccgct
    32461 gatttgcatc actggtcagg ttcccgcctc gatgatcggc accgacgcct tccaggaagt
    32521 cgatacctac ggcatctcta tccccatcac caaacacaac tatctggtca gacatatcga
    32581 agaactcccg caggtcatga gcgatgcctt ccgcattgcg caatcaggcc gccctggccc
    32641 ggtgtggata gacattccta aggatgtaca aacggcagtt tttgagattg aagctcagcc
    32701 cgcggtggca gaaaaagccg ctgcacccgc ctttagcgaa gaaagcattc gtgacgcggc
    32761 tgcgatgatt aacgccgcca aacgcccggt gctttatctg ggcggcggtg tgatcaatgc
    32821 gcctgtgcgg gtgcgtgaac tggcggagaa agcgcaactg ccaactacca tgaccttaat
    32881 ggcgctgggt atgctgccaa aagcgcatcc gctgtcgctg ggtatgctgg ggatgcacgg
    32941 cgtgcgcagc accaactata ttttgcagga ggcggatttg ttgattgtgc tcggtgcgcg
    33001 ttttgatgac cgggcgattg gcaaaaccga gcagttctgc ccgaatgcca aaattattca
    33061 tgtcgatatc gaccgcgcag agctgggtaa aatcaagcaa ccgcatgtgg cgattcaggc
    33121 ggatgttgat gacgtgctgg cgcagttgat cccgctggtg gaagcgcaac cgcgtgcaga
    33181 gtggcaccag ttggtggcgg atttgcagcg cgaatttccg tgtccaatcc cgaaagcgtg
    33241 cgatccatta agccattacg gcctgatcaa cgccgttgcc gcctgtgtcg atgacaatgc
    33301 gattatcacc accgacgttg gtcagcatca gatgtggacc gcgcaagcct atccgctcaa
    33361 tcgcccacgc cagtggctga cctctggcgg gctgggcacg atgggctttg gcctgcctgc
    33421 ggcgattggc gcggcgttgg cgaacccgga tcgcaaagtg ttgtgtttct ccggcgacgg
    33481 cagcctgatg atgaatattc aggagatggc gaccgccagt gaaaaccagc tggatgtcaa
    33541 aatcattctg atgaacaacg aagcgctggg gctggtgcat cagcaacaga gtctgttcta
    33601 caagcagggc gtttttgccg ctacctatcc gggaaaaatc aactttatgc agattgccgc
    33661 cggattcggc ctcgaaacct gtgatttgaa taacgaagcc gatccgcagg ctgcattgca
    33721 agaaatcatc aatcgccctg gtccggcgct gatccatgtg cgtattgatg ccgaagaaaa
    33781 agtgtacccg atggtgccgc caggtgcggc gaatactgaa atggtggggg aataagccat
    33841 gcaaaacaca actcatgaca acgtgattct ggaactcacc gttcgcaacc atccgggcgt
    33901 aatgacccac gtttgtggcc tttttgcccg tcgcgctttt aacgttgagg gcattctttg
    33961 cttgccgatt caggacagcg acaaaagcca catttggcta ctggtcaatg acgaccagcg
    34021 tctggagcag atgataagcc agatagacaa gctggaagat gtggtgaaag tgcagcgtaa
    34081 tcagtccgat ccgacgatgt ttaacaagat tgcggtgttt tttcagtaag gagcgacatt
    34141 aaaccgctcg ggggggtact gatgagcgat ttttgataga tccgttcaga tatatagggt
    34201 gaaattgtgc cagagaccat ctcaggcaca atttattagc cctgaatgtt aatttaagtc
    34261 gctgcaccag taatttgggg gttcacagtt agggctaaaa tttccagcga tggtgttaaa
    34321 ttctgattgc tgtctatacc aatggtcatg accagcattc tattaattaa tggttcatcg
    34381 tgaggtatat tatggcaagc cctataaaca gcggaataat gatgtcaaat ctctgctctt
    34441 ccaccttttt ggataaaaat ataaatgtcg ctgctgagct tgatattaca aatagcgaga
    34501 aaaaatattc gcccggcagt aattttgcaa aatggatgtt gcaggaaata aagcgcttaa
    34561 tagcaaatat tatgtccggt tctcgcagtg ttaacaccga aatccttaac tatttccacc
    34621 ctatgccagg cactgaaaat aatggcaatc gtacctggat ggcggcaact ggagagaacg
    34681 aatataccgt aatcgaacaa acaggtggca aatcctttaa agttatacag tttgatgcag
    34741 gtgaaaaaaa tacaaaaaaa atagtaaaag aaacatcata ttctggagtg gctgtggcga
    34801 ctattatcaa atcgctgtct gaaaaaactg ctgcgttaga aacacattct gctgacacgg
    34861 ttttgaggaa aaaattagtt aatagtattg ttatgaagaa tacggatttt aaacacgtaa
    34921 ttactgcaga tattgagaat aatatatatg at
//
LOCUS       DAZGBC010000033        34504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGBC010000033 DAZGBC010000000
VERSION     DAZGBC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.033"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..1531
                     /gene="sthA"
                     /locus_tag="ACHK1J_001641"
     CDS             131..1531
                     /gene="sthA"
                     /locus_tag="ACHK1J_001641"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO7910930.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(1514..2431)
                     /gene="oxyR"
                     /locus_tag="ACHK1J_001642"
     CDS             complement(1514..2431)
                     /gene="oxyR"
                     /locus_tag="ACHK1J_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO7910931.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2698..4071)
                     /gene="argH"
                     /locus_tag="ACHK1J_001643"
     CDS             complement(2698..4071)
                     /gene="argH"
                     /locus_tag="ACHK1J_001643"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO7910932.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            complement(4132..4908)
                     /gene="argB"
                     /locus_tag="ACHK1J_001644"
     CDS             complement(4132..4908)
                     /gene="argB"
                     /locus_tag="ACHK1J_001644"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO7910933.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(4916..5920)
                     /gene="argC"
                     /locus_tag="ACHK1J_001645"
     CDS             complement(4916..5920)
                     /gene="argC"
                     /locus_tag="ACHK1J_001645"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO7910934.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWSGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAQTQSLI
                     "
     gene            6074..7225
                     /gene="argE"
                     /locus_tag="ACHK1J_001646"
     CDS             6074..7225
                     /gene="argE"
                     /locus_tag="ACHK1J_001646"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO7910935.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     NLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHLSNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            7823..10474
                     /gene="ppc"
                     /locus_tag="ACHK1J_001647"
     CDS             7823..10474
                     /gene="ppc"
                     /locus_tag="ACHK1J_001647"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO7910936.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            10657..12375
                     /gene="eptC"
                     /locus_tag="ACHK1J_001648"
     CDS             10657..12375
                     /gene="eptC"
                     /locus_tag="ACHK1J_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO7910937.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGD"
     gene            12605..13456
                     /gene="yijO"
                     /locus_tag="ACHK1J_001649"
     CDS             12605..13456
                     /gene="yijO"
                     /locus_tag="ACHK1J_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7910938.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTAKLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(13443..13784)
                     /gene="frwD"
                     /locus_tag="ACHK1J_001650"
     CDS             complement(13443..13784)
                     /gene="frwD"
                     /locus_tag="ACHK1J_001650"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7910939.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGISIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13786..14664)
                     /gene="pflC"
                     /locus_tag="ACHK1J_001651"
     CDS             complement(13786..14664)
                     /gene="pflC"
                     /locus_tag="ACHK1J_001651"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO7910940.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(14630..16927)
                     /gene="pflD"
                     /locus_tag="ACHK1J_001652"
     CDS             complement(14630..16927)
                     /gene="pflD"
                     /locus_tag="ACHK1J_001652"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7910941.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAAGCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(16978..17298)
                     /gene="frwB"
                     /locus_tag="ACHK1J_001653"
     CDS             complement(16978..17298)
                     /gene="frwB"
                     /locus_tag="ACHK1J_001653"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7910942.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(17313..18392)
                     /gene="frwC"
                     /locus_tag="ACHK1J_001654"
     CDS             complement(17313..18392)
                     /gene="frwC"
                     /locus_tag="ACHK1J_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO7910943.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAICVNVL
                     KSLARKKGSSTDEKEDDLDLDFEIN"
     gene            18701..21202
                     /gene="ptsP"
                     /locus_tag="ACHK1J_001655"
     CDS             18701..21202
                     /gene="ptsP"
                     /locus_tag="ACHK1J_001655"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7910944.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            21214..21876
                     /gene="fsa"
                     /locus_tag="ACHK1J_001656"
     CDS             21214..21876
                     /gene="fsa"
                     /locus_tag="ACHK1J_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7910945.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDKGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            21887..22990
                     /gene="gldA"
                     /locus_tag="ACHK1J_001657"
     CDS             21887..22990
                     /gene="gldA"
                     /locus_tag="ACHK1J_001657"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO7910946.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            23264..23881
                     /gene="yijF"
                     /locus_tag="ACHK1J_001658"
     CDS             23264..23881
                     /gene="yijF"
                     /locus_tag="ACHK1J_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO7910947.1"
                     /translation="MKASLVLVSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIEDGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(23908..24813)
                     /gene="yijE"
                     /locus_tag="ACHK1J_001659"
     CDS             complement(23908..24813)
                     /gene="yijE"
                     /locus_tag="ACHK1J_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO7910948.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(24907..27087)
                     /gene="katG"
                     /locus_tag="ACHK1J_001660"
     CDS             complement(24907..27087)
                     /gene="katG"
                     /locus_tag="ACHK1J_001660"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO7910949.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(27416..28306)
                     /gene="metF"
                     /locus_tag="ACHK1J_001661"
     CDS             complement(27416..28306)
                     /gene="metF"
                     /locus_tag="ACHK1J_001661"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO7910950.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            28382..28627
                     /locus_tag="ACHK1J_001662"
     CDS             28382..28627
                     /locus_tag="ACHK1J_001662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7910951.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     LTNHVLTASCRIRRKRFIRPNPMIIQYISLETIDPKT"
     gene            complement(28655..31087)
                     /gene="metL"
                     /locus_tag="ACHK1J_001663"
     CDS             complement(28655..31087)
                     /gene="metL"
                     /locus_tag="ACHK1J_001663"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO7910952.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(31090..32250)
                     /gene="metB"
                     /locus_tag="ACHK1J_001664"
     CDS             complement(31090..32250)
                     /gene="metB"
                     /locus_tag="ACHK1J_001664"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO7910953.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            32527..32844
                     /gene="metJ"
                     /locus_tag="ACHK1J_001665"
     CDS             32527..32844
                     /gene="metJ"
                     /locus_tag="ACHK1J_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO7910954.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            32904..33512
                     /gene="yiiX"
                     /locus_tag="ACHK1J_001666"
     CDS             32904..33512
                     /gene="yiiX"
                     /locus_tag="ACHK1J_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO7910955.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            33657..34332
                     /locus_tag="ACHK1J_001667"
                     /pseudo
     CDS             33657..34332
                     /locus_tag="ACHK1J_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1H family transposase"
     gene            complement(34419..>34504)
                     /locus_tag="ACHK1J_001668"
     CDS             complement(34419..>34504)
                     /locus_tag="ACHK1J_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO7910956.1"
                     /translation="GGGGMSYAYEYDVKYNICHLRKKSRMR"
BASE COUNT         8323 a         9649 c         8736 g         7796 t
ORIGIN      
        1 taagaaggag gcagacaaag caaaggccgc tcacgctata gccagattaa tgacggggat
       61 caattggctt acccgcgata aaatgttacc attctgttgc ttttatgtat aagaacaggt
      121 aagccctacc atgccacatt cctacgatta cgatgccata gtaataggtt ccggccccgg
      181 cggcgaaggc gctgcaatgg gcctggttaa gcaaggtgcg cgcgtcgcag ttatcgagcg
      241 ttatcaaaat gttggcggcg gttgcaccca ctggggcacc atcccgtcga aagctctccg
      301 tcacgccgtc agccgcatta tagaattcaa tcaaaaccca ctatacagcg accattcccg
      361 actgctccgc tcttcttttg ccgatatcct taaccatgcc gataacgtga ttaatcaaca
      421 aacgcgcatg cgtcagggat tttacgaacg taatcactgt gaaatattgc aggggaacgc
      481 tcgctttgtt gacgagcata cgttggcgct ggattgcccg gacggcagcg ttgaaacact
      541 aaccgctgaa aaatttgtta ttgcctgcgg ctctcgtcca tatcatccaa cagatgttga
      601 tttcacccat ccacgcattt acgacagcga ctcaattctt agcatgcacc acgaaccgcg
      661 ccatgtactt atctatggtg ctggagtgat cggctgtgaa tatgcgtcga tcttccgcgg
      721 tatggatgta aaagtggatc tgatcaacac ccgcgatcgc ctgctggcat ttctcgatca
      781 agagatgtca gattctctct cctatcactt ctggaacagt ggcgtagtga ttcgccacaa
      841 cgaagagtac gagaagatcg aaggctgtga cgacggtgtg atcatgcatc tgaagtcggg
      901 taaaaaactg aaagctgact gcctgctcta tgccaacggt cgcaccggta atactgattc
      961 gctggcgtta cagaacattg ggctggaaac tgacagtcgc ggacagctga aggtcaacag
     1021 tatgtatcag accgcacagc cgcacgttta cgcggtgggc gacgtgattg gttatccgag
     1081 cctggcgtcg gcggcctatg accaggggcg cattgccgcg caggcgctgg tgaaaggtga
     1141 agccaccgca catctgattg aagatatccc taccggtatt tacaccatcc cggaaatcag
     1201 ctctgtgggc aaaaccgaac agcagctgac cgcgatgaaa gtaccgtatg aagtgggccg
     1261 cgcccagttc aaacatctgg cacgcgcaca aatcgtcggc atgaacgtgg gcacgctgaa
     1321 aattttgttc catcgggaaa cgaaagagat tctgggcatt cactgctttg gcgagcgcgc
     1381 tgccgaaatt attcatatcg gccaggcgat tatggaacag aaaggtggcg gcaacactat
     1441 tgagtacttc gtcaacacca cctttaacta cccgacgatg gcggaagcct atcgggtagc
     1501 tgcgctaaat ggcttaaacc gcctgtttta acactttatc gaaatggcca tccattcttg
     1561 cgcggatggc ctctgccagc tgctcatagc ggctgcgcag cggtgagcca ggacgataaa
     1621 ccaggccaat agtgcggcga ggttccggct taatacacgg cagataaaca accccatcgc
     1681 gtttgcgctc cggcggcaca gccagcgcag gcagtaaagt gatcccgcta cctgccgcca
     1741 ccatgttgcg cagagtttcc aggctggtcg cgcggaagtg tgtatcttca tccgccccgg
     1801 cttcaaaaca gaaacccatt gcctgatcgc gcaaacagtg accatcttcc agcatcagca
     1861 gtttttcccc agccagatcg gccatcggta cgcattcgcg gttcgcccac gggtggtctt
     1921 catagatagc cagcaacatt ggctcatcaa acaacggcac ttcaatgaat gcttcgctct
     1981 ctttcaccag cgcgaggatc acgcaatcga gtttgccgct gtccagttgc gccagtaact
     2041 ggtgggtctg tgcttcatgc agatacattt ccagctttgg aaaggtctgg tgcagcatcg
     2101 ggataatatg cggtagcagg tacggtccaa ccgtgggaat caaaccaata tgcagcggtc
     2161 cggacatcgt ctcgccctgc tggcttgcca tctctttaag gactttcacc tcacgcagca
     2221 cggtacgcgc ctgatccacc agcagcattc ccgcctgggt gaacaacact ttacggctgg
     2281 tccgctccag caacatcacg cccagctcat cttccagctt acgaatttgc ccgctaagcg
     2341 tcggctggct aacgtggcag gaatctgccg cacgccgaaa atggcggtgt tcagccaatg
     2401 ccaccaggta ctcaagatca cgaatattca ttatccatcc tccatcgcca cgatagttca
     2461 tggcgatagg tagaatagca atgaacgatt atccctatca agcattctga ctgataattg
     2521 ctcacagaaa cggagcggca cctcttttaa cccttgaagt cactgcccgt ttcgagagtt
     2581 tctcaactcg aataactaaa gccaacgtga acttttgcgg atctccagga tccgcttttt
     2641 tttgccataa aaaagcccgg cgataagcca ggctcaaatt tgtacatata aatgttctta
     2701 ccccaaccgc gcccttgcaa aagcaatcgc ctgcgccacc tgctgcggtg agacgccgcc
     2761 ttttgccgca cgcttgtcga ggcacgattg cagcgacaaa atcggataga catcttcgcc
     2821 aatcaccggg ctgaatttct gcaactcgtc gagcggcagt tcttccagcg gtttgccctg
     2881 acgaatggct tccaccaccg cttcaccaac aatatggtgc gcctcgcgga aaggtacgcc
     2941 tttcgccacc aggtaatccg ccagttcggt ggcgttggcg taaccctgct gagccgcttc
     3001 ctggcaacgt ggacgtttca cctggatgcc gtccagcacc agcgccgcca tatgcaggca
     3061 gtccagccag gtatcgagcg cgtcgaacag accttctttg tcttcctgca tatctttgtt
     3121 gtaagccagc ggcaaacctt tcagcgtcat catcatgccg gttaacgcgc cctgcacccg
     3181 accgcattta ccgcgaatca gctccagcgc atccgggttt ttcttctgcg gcattaatga
     3241 tgaaccggaa gtcacgcggt cagaaagctc cacaaacccc gcttcgccgg tgttaaagaa
     3301 aatcagatct tcagcaaaac gcgacagatg caccatgccg atagcggcag ccgacagcag
     3361 ttccagcacg tggtcacggt cagaaacgct gtcgagactg ttacgggtcg ctgaagcaaa
     3421 gcccagccag ccagctaact gttcacggtc gatctcataa gccgttcccg ccagcgcgcc
     3481 acagcctaac ggactgacat ccagacgctt aagcgcatcc tgcaaacggc tttcatcacg
     3541 cgccagcatc tcaacatagg ccaggcacca gtgcgcgaac gtcaccggct gggcgcgttg
     3601 caggtgagtg taacctggca tgaccgcgtc ctgattgttt tgtgcggttt ccaccagcgc
     3661 cgattgcagc tgccggttag ccgtcagtaa ctcactaacg gtatctttgc accacagttt
     3721 caggtcagtc gctacctgat cattacgact acgcccggta tgcagctttt tgcctaactg
     3781 gcccactttg tcgatcagtt tgccttccac ccagctatgg atatcttcgg cgtcgctttc
     3841 aaggatttgt tgtggcctgg cgcgaacatc ttccagcagc acgttcagcg cctcttccag
     3901 ttgagcctgc tcttctgcgg ttaacacgcc taccgtgacc agggctttgg accaggccac
     3961 agagccaaca atatcctgct ccgccagacg gtaatcaaag cgcagtgagt cgttgaattg
     4021 tttgaaccgt tgatctgctg cctgggtaaa acgcccgccc caaagtgcca taactctgtt
     4081 tccttatttt gaaattcaat gccggaaaac tgcgcctccg gccaacaaaa cttaagctaa
     4141 aatccgcgta cccatcggca taccgttaaa cagtgccgga agctgctccg catgacgcca
     4201 ggaggcgata tctaccggac ggcccaacgt gcgcgcagca tccagcgccg cgttcacttt
     4261 cactatcatg ccgtcagtaa taatgccctg ctcaatcagt tgttctgctt tcgcggcggt
     4321 catttcggca atgcgttgcc ctttgccgtc gagaatgcca ctgacgtcgg agagcaaaat
     4381 cagatccgcg cccagcgttg ccgccagcgc cgttgccgcc tggtcggcat tgacgttcat
     4441 cagttgccct tcgtctgtta cgccaatgga gctgaccacc ggcagataac cgttctccag
     4501 caaggtgttg ataagcttag gcgaacctgg ctgcgccagt ccaacatggc ctaactcttc
     4561 atcaagctgg gtcactttga cgctgtcgcc gtcaccgaga aacaaaccta cggccgcaat
     4621 ctgatgtttc ttcgcccatg ccagcagggt tttatttgcc gttcccgcca gtgctccagt
     4681 gataatgtct atctgatccg caggcgtcac ccgcaggcca tttttctttt tcaccggcag
     4741 attcagccct ttcatcagct catccaccac gcaaccgccg ccgtggacaa tcaccagcgg
     4801 acgctgatgt gactcacgat aattcaccag tgcgctaaac agacgctcca gcgcctcttc
     4861 actatccagc agtacgccac ccagtttgat aattaatgga ttcatcattg cacccttaaa
     4921 taagagactg cgtttgggca tagccgaaac gaatattggc gcactgtacc gcttgtgccg
     4981 ccgcgccttt cagtaagttg tcttcggtcg ccacaataat caaatgctcg ccctgaacgg
     5041 caaacccgat atcgcaaaat ggcagcccaa cgacattttt cagcgccgga acgcctttgt
     5101 catacagccg caccagcggt ttatgggcat acgcctgctg taacacttgc gcgacttgcg
     5161 cctgggtcac acccggtttc aggcggcagg taatggtttc gagaatgccg cgtgggaaat
     5221 tgcccagatg cggggtgaag ataacgtcag caccgaggtg tgtggcgatc tctggttgat
     5281 ggcgatgagt aaagacgcca tacggttgca ggctaacttc acaaaaacta tttgaaatcg
     5341 ctgccttacg tcctgcaccg ctcacgccgc tggtggcgtt gatcaccggc cactgattga
     5401 ggtcaagaag atcggcatca atcaacggtt tcagcgccag ctgtgccgcc gtcggataac
     5461 agcctggcac cgcaatcaaa ttcgcttctt ttaatttatt accgctccac tccgccagac
     5521 cgtaggctgc ctgttccagc agttccgggt attgatgggt aaagccgtaa tatttttcat
     5581 agaaggtggc gtcgttaaca cgaaacgcgc cggaaaggtc gaacaccacg cagcccgctt
     5641 caagaaactg cggcgctaaa tcgtggctaa cttcatgggc ggtggcgaga aacactacgt
     5701 ccacccctgg gctaaactcg ctgatatccg acatcggctg caacggcaga tcaacgatgc
     5761 cttttagctg cggatgcaaa tcggagatta actttcccgc atcattgctt tgcgctgaaa
     5821 cagtcaaagc ggttatgttc atatgcggat ggcgatttac ataggtcact agctctgcgc
     5881 cagcgtagcc gctggcaccc acaatcagcg tattcaacat cggggctatt caccttctta
     5941 tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg gctggtgggt tttattacgc
     6001 tcaacgttag tgtattttta ttcataaata ctgcatgaat attgatacta tcatgaccag
     6061 aggtgtgtca acaatgaaaa acaaattacc gccatttatc gagatttacc gtgctctgat
     6121 tgccacacct tcaataagcg ccacggaaga ggcactcgat caaagcaatg cagatttaat
     6181 cactctgctg gcggactggt ttaaaaattt aggcttcaat gtggaagtgc agcctgtgcc
     6241 aggaactcgc aacaaattca atatgctggc aagtaccgga cagggggctg gcggcttgtt
     6301 gctggcgggg cataccgata cggtgccatt tgatgacggt cgctggacgc gcgatccgtt
     6361 tacactgacg gagcatgacg gcaagcttta cggcttaggc accgccgaca tgaaaggctt
     6421 ttttgcgttt atccttgatg cgctacgcga tgtcgacgtc acgaaactga aaaaaccgct
     6481 ctacattctg gcgactgctg atgaagaaac cagtatggcc ggagcgcgtt attttgccga
     6541 aactaccgcc ctgcgcccgg attgcgccat cattggcgaa ccgacgtcac tacaaccggt
     6601 acgcgcacat aaaggtcatc tctctaacgc catccgtatt cagggtcagt cggggcactc
     6661 cagcgatcca gcacgcggag ttaacgctat cgaactaatg cacgacgcca tcgggcatat
     6721 tttgcaattg cgcgataacc tgaaagaacg ttatcactac gaagcgttta ccgtgccata
     6781 ccctacgctc aacctcgggc atattcacgg tggcgacgcg tctaaccgta tttgcgcttg
     6841 ctgtgagttg catatggata ttcgtccgct gcctggcatg acactcaatg aacttaatgg
     6901 tttgctcaac gatgcattgg ctccggtgag cgaacgctgg ccgggtcgtc tgacggtcga
     6961 cgagctgcat ccgccgatcc ctggctatga atgcccgcca aatcatcaac tggttgaagt
     7021 agttgagaaa ttgctcggag caaagaccga agtggtgaac tactgtaccg aagcgccgtt
     7081 tattcaaacg ttatgcccga cgctggttct ggggccgggt tcaatcaatc aagcccatca
     7141 gcctgatgaa tatctggaga cgaggtttat caagcccacc cgcgaactga tcacccaggt
     7201 gattcaccat ttttgctggc attaaaacgt aggccgaata aggcgcttgc gccgcatccg
     7261 gcactgttgc caaactccag tgccgcaata atgtcggatg cgatgcttgc gcatcttatc
     7321 cgacctacag tgactcaaac gatgcccaac cgtaggccgg ataaggcgct cgcgccgcat
     7381 ccggcactgt tgccaaactc cagtgccgca ataatgtcgg atgcgatact tgcgcatctt
     7441 atccgaccta cagtgactca aacgatgccc aaccgtaggc cggataaggc gctcgcgccg
     7501 catccggcac tgttgccaaa ctccagtgcc gcaataatgt cggatgcgat acttgcgcat
     7561 cttatccgac ctacaccttt ggtgttactt gaggcgattt tttaacattc ccataagtta
     7621 cgcttattta aagcgtcgtg aatttaatga cgtaaattcc tgctatttat tcgtttgctg
     7681 aagcgatttc gcagcatttg acgtcaccgc ttttacgtgg ctttataaaa gacgacgaaa
     7741 agcaaagccc gagcatattc gcgccaatgc gacgtgaagg atacagggct atcaaacgat
     7801 aagatggggt gtctggggta atatgaacga acaatattcc gcattgcgta gtaatgtcag
     7861 tatgctcggc aaagtgctgg gagaaaccat caaggatgcg ttgggagaac acattcttga
     7921 acgcgtagaa actatccgta agttgtccaa atcttcacgc gctggcaatg atgctaaccg
     7981 ccaggagttg ctcaccacct tacaaaattt gtcgaacgac gagctgctgc ccgttgcgcg
     8041 tgcgtttagt cagttcctga acctggccaa caccgccgag caataccaca gcatttcgcc
     8101 gaaaggcgaa gctgccagca acccggaagt gatcgcccgc accctgcgta aactgaaaaa
     8161 ccagccggaa ctgagcgaag acaccatcaa aaaagcagtg gaatcgctgt cgctggaact
     8221 ggtcctcacg gctcacccaa ccgaaattac ccgtcgtaca ctgatccaca aaatggtgga
     8281 agtgaacgcc tgtttaaaac agctcgataa caaagatatc gctgactacg aacacaacca
     8341 gctgatgcgc cgcctgcgcc agttgatcgc ccagtcatgg cataccgatg aaatccgtaa
     8401 gctgcgtcca agcccggtag atgaagccaa atggggcttt gccgtagtag aaaacagcct
     8461 gtggcaaggc gtaccgaatt acctgcgcga actgaacgaa caactggaag agaacctcgg
     8521 ctacaaactg cccgtcgaat ttgttccggt acgttttacc tcgtggatgg gcggtgaccg
     8581 cgacggcaac ccaaacgtca ctgccgatat cacccgccac gtcctgctgc tcagccgttg
     8641 gaaagccacc gatttgttcc tgaaagatat tcaggtgctg gtttctgaac tgtcgatggt
     8701 tgaagcgacc cctgaactgc tggcgctggt tggcgaagaa ggtgccgcag aaccgtatcg
     8761 ctatctgatg aaaaacctgc gttctcgcct gatggcgaca caggcatggc tggaagcgcg
     8821 cctgaaaggc gaagaactgc caaaaccaga aggcctgctg acacaaaacg aagaactgtg
     8881 ggaaccgctc tacgcttgct accagtcact tcaggcgtgt ggcatgggta ttatcgccaa
     8941 cggcgatctg ctcgacaccc tgcgccgcgt gaaatgtttc ggcgtaccgc tggtccgtat
     9001 tgatatccgt caggagagca cccgccatac cgaagcgctg ggcgagctga cccgctacct
     9061 cggtatcggc gactacgaaa gctggtcaga ggccgacaaa caggcgttcc tgatccgcga
     9121 actgaactcc aaacgtccgc ttctgccgcg caactggcaa ccaagcgccg aaacgcgcga
     9181 agtgctcgat acctgccagg tgattgccga agcgccgcaa ggctccattg ccgcctacgt
     9241 gatctcgatg gcgaaaacgc cgtccgacgt gctggctgtc cacctgctgc tgaaagaagc
     9301 gggtatcggg tttgcaatgc cggttgctcc gctgtttgaa accctcgatg acctgaacaa
     9361 cgccaacgat gtcatgaccc agctgctgaa tatcgactgg tatcgcggcc tgattcaggg
     9421 caaacagatg gtgatgattg gctattccga ctcagcaaaa gatgcgggcg tgatggcagc
     9481 ttcctgggcg caatatcagg cacaggatgc attaatcaaa acctgcgaaa aagcgggtat
     9541 tgagctgacg ttgttccacg gtcgcggcgg ttccattggt cgcggcggcg cacctgctca
     9601 tgcggcgctg ctgtcacaac cgccaggaag cctgaaaggt ggcctgcgcg tgactgaaca
     9661 gggcgagatg atccgcttta aatatggtct gccagaaatc accgtcagca gcctgtcgct
     9721 ttacaccggg gcgattctgg aagccaacct gctgccacca cctgagccga aagagagctg
     9781 gcgtcgcatt atggatgaac tatcagtcat ctcctgcgat ctctaccgtg gctacgtacg
     9841 tgaaaacaaa gattttgtgc cttacttccg ctccgctaca ccggaacaag aactgggcaa
     9901 actgccgttg ggttcacgtc cggcgaaacg tcgcccgacc ggcggcgtcg agtcgctgcg
     9961 cgccattccg tggatttttg cctggacgca aaaccgtctg atgctccccg cctggctggg
    10021 tgcaggtaca gcgctgcaaa aagtggtcga agatggcaaa cagagcgagc tggaggctat
    10081 gtgccgcgat tggccattct tctcgacgcg tctcggcatg ctggaaatgg tattcgccaa
    10141 agcagatctg tggctggcgg aatactatga ccaacgcctg gtagacaaag cactgtggcc
    10201 gttaggtaaa gagttacgca atctgcaaga agaagacatc aaagtggtgc tggcgattgc
    10261 gaacgattcc catctgatgg ccgatctgcc gtggattgca gagtctattc agctacggaa
    10321 tatttacacc gacccgctga acgtattgca agccgagttg ctgcaccgct cccgccaggc
    10381 agaaaaagaa ggccaggaac cggatcctcg cgtcgagcag gcgttaatgg tcactattgc
    10441 cgggattgcg gcaggtatgc gtaataccgg ctaatcttcc tctcctgcaa accctcgtgc
    10501 ttttgcgcga gggttttctg aaatacttct gttctaacac cctcgttttc aatatatttc
    10561 tgtctgtatt ttattcaaat tctggatatc ccttcagata tccttaagga attgtcggta
    10621 cattcggcga tatttttttc aagacaggtt cttactatgc attccacaga agtccaggct
    10681 aaacctcttt ttagctggaa agccctgggt tgggcactgc tctacttttg gtttttctct
    10741 actctgctac aggccattat ttacatcagt ggttatagtg gcaccaacgg cattcgcgac
    10801 tcgctgttat tcagctcgct gtggttaatt ccggtattcc tctttccgaa gcggatcaaa
    10861 attattgccg cagtgatcgg cgtggtgcta tgggcggcct ctctggcggc gctgtgctac
    10921 tacgtcatct acgggcagga gttctcgcag agcgttctgt ttgtgatgtt cgaaaccaac
    10981 accaacgaag ccagcgagta tttaagccag tatttcagcc tgaaaattgt gcttatcgcg
    11041 ctggcctata cggcagtggc agttctgctg tggacacgtc tgcgcccggt ctatattcca
    11101 aagccgtggc gttatattgt ctcttttgcc ctgctttatg gcttgattct gcatccgatc
    11161 gccatgaata cgtttatcaa aaacaagccg tttgagaaaa cgttggataa cctggcctcg
    11221 cgtatggagc ctgccgcacc gtggcaattc ctgaccggct attatcagta tcgtcagcaa
    11281 ctaaactcgc taacaaagct actgaatgaa aataatgcct tgccgccgct ggctaatttc
    11341 aaagatgaat cgggtaacga accgcgcacc ttagtgctgg tgattggcga gtcaacccag
    11401 cgtggacgca tgagtctgta cggttatccg cgtgaaacca cgccggagct ggatgcgctg
    11461 cataaaaccg atccgaatct aaccgtgttt aataacgtag ttacgtctcg tccgtacacc
    11521 attgaaatcc tgcaacaggc gctgaccttt gccaatgaaa agaaccctga tctgtatctg
    11581 acgcagccgt cgctgatgaa catgatgaaa caggcgggtt ataaaacctt ctggatcacc
    11641 aaccagcaga cgatgaccgc ccgcaatacc atgctgacgg tattttcgcg ccagaccgac
    11701 aagcagtact acatgaacca gcaacgtacg cagagtgcgc gtgaatacga cactaacgtg
    11761 ctgaagccgt tccaggaagt gctgaaggac cctgcgccga agaaactgat cattgttcat
    11821 ctgctgggta cgcatatcaa atacaaatac cgctacccgg aaaatcaggg caagtttgat
    11881 ggcaataccg atcatgttcc accgggatta agcgcagaag aactggagtc atataacgat
    11941 tatgacaacg ctaacctgta taacgatcat gtggttgcca gcctgattaa agactttaaa
    12001 gcggcagacc cgaacggatt cctggtttac ttctctgacc acggtgaaga ggtttacgac
    12061 acaccgcctc ataaaaccca ggggcgtaat gaggacaacc cgacgcgtca tatgtacacc
    12121 attccgttcc tgctgtggac gtcagaaaaa tggcaagcga ctcatccgcg tgatttctcg
    12181 caggatgttg atcgtaaata cagccttgcg gaactgatcc acacctggtc agatttagca
    12241 ggcttatctt acgacggtta cgatccaacc cgttcagtgg tgaatccgca gttcaaagaa
    12301 actacccgct ggattggtaa cccgtacaag aaaaacgcgc tgatcgatta cgacacgctg
    12361 ccatatggcg actaggtagg taatcagtaa ttatcagcca ggcgctattt gccggatgcg
    12421 acgcttgacg cgtcttatcc ggcctacaga tcgcggcggt agactggata aggcgtttac
    12481 gccacatccg gcaatacaac tgccgcaatg tttacatcga actcccctca ggccacactt
    12541 ttcaggtggc cttttttaat aatgccagtt actgttttcc cgtctacata cctaagggaa
    12601 taacatgtat cacgacgtca gctatctgct ctcccgcctg atcaacggcc cgctgtccct
    12661 gcgccagatt tactttgcca gcagtaacgg cccggtgccc gatcttgcgt atcaggtcga
    12721 ttttccacgg ctggaaattg tgctggaagg tgagtttgtt gataccggcg ctggagcaac
    12781 gttagttccc ggcgatgtgc tgtacgttcc tgctggtggc tggaattttc cacaatggca
    12841 agcccccgcc actaccttta gcgtgctgtt tggcaaacag caactcggct tcagcgtcgt
    12901 gcaatgggat ggcaaacaat atcaaaatct ggcgaagcaa cacgtcgccc gacgcggccc
    12961 acgcataggt tcttttctgc tacaaacgct caatgaaatg caaatgcagc cgcaggagca
    13021 gcaaacggca aagcttatcg tcgccagcct gcttagccac tgccgcgatt tgctcggcag
    13081 ccaaatccag accgcctcac gcagccaggc actgttcgaa gctattcgtg attatatcga
    13141 cgaacgctat gcctccgcgc ttacccgcga atctgtcgca caggcattct atatttcacc
    13201 aaactatctc tcgcacctgt ttcaaaaaac gggggccatt ggttttaacg aatacctgaa
    13261 tcacacgcga ctggagcacg ctaagacgtt attgaaaggc tatgacctga aggtaaaaga
    13321 agtggcgcac gcctgcggct ttgtcgacag caactacttc tgccgtctgt ttcgtaaaaa
    13381 caccgaacgc tcgccgtcgg aatatcgtcg gcagtaccac agccaactga cagaaaaacc
    13441 gactactcca gaataagatg ggtttgctgc ggcgcagaaa gcactttgcg caccgcgctc
    13501 attacccgct gcggctcacg caggaatgcg tagatgctgc attgcacata gcggcaatgt
    13561 tcaaatcgct cggcaccggc aagctcgata tccgtaatca acagagcaac atccgcccga
    13621 cggatatcct cgtccgctaa acgattctcc gttcccagcg cgccctgagt ttcaatgctg
    13681 atgccccact tctctaactg gcacaacttt tccagccgtt ccgccgccat ataagtatgc
    13741 gccacgccgc ttacgcaggc ggttactgcc accaggtatg ccattttaac ctcccacggt
    13801 aacctgaaat ccggcccgtt ctgccatttc gcgcatcgtt gccacatcag ctgacgacgg
    13861 cgcaggcacc tctttcatcg accatgtttt ccccagcagg cggtatttcg gttcgccgta
    13921 ctgatgaaac ggtaacagat ggatctgcct gatattcagc gggatcagca cgtccagcgc
    13981 ctgctgcata ttctcccggc tgagcgtgaa accagggatc agcggtaaac gcgggatcac
    14041 gttgacgccc tcactcacca gcaaacgcag attctccagc acgcgtggca ggttcatctt
    14101 caccacatcc cgcgcctgag tcgcgtccat aatttttaaa tcgaacaaca cttcatcgca
    14161 caatttcgcc agtggtaaca gcttgctggc tggtgcgtct ccggcagttt caatggcgca
    14221 tgacacaccc cacagccgca gtcgctgtaa aaaacgggta gcaaactccg cctgcattaa
    14281 cacttcgccg ccagaaagcg tgacgccgcc gccggacgtg cgaaagaaaa tgtcatcttt
    14341 catcacttcc cgttccagcg cgtcaaggct gatatcgcga ccaatccgtt caaacgcccc
    14401 ggaggggcat tcatccgcat cacgcaaaca tttcgcacag tgcagacatt tcgcctctct
    14461 gcgtacagtc tggattttgc cggagatcga ctccggatta gcacaccacg ggcaaagatg
    14521 tggacagcct ttaaaaaaga ccaccgtacg aatgccctcg ccgtcattca gtgaataacg
    14581 ctgaatgttg aaaatgcgcg ccacatcatt gcggcgcgtt tccacaacgt tacagctgat
    14641 gcgctgtccg gcggatgatg tcatcctgga tctccttcga cagttcgaca aagaaggcgc
    14701 tgtatccggc aacgcgcacc accagcccgg cataatcttg tgggcgctgt tgcgcttccc
    14761 gcaacgtgtc ggcgttcacc acgttaaact gaatatgttg taacttaagc tgggtaaacg
    14821 cccgtaagaa gtcggccagt ttgcgtaatc ccgcttcacc ttccagggtc gccggagtga
    14881 atttcacgtt cagcaacgta ccgttagaca gcagcgtatt atcgagctta ctgactgact
    14941 tcagtaccgc tgttggccct tgtgcgtcct ggcccagcat gggtgacaag ccgccgtctg
    15001 ccagctgttc tccggcaaaa cgaccgtctg gcgtcgcgcc aaccaccgat cccaacggga
    15061 cgtgagcaga aacggtatac gatcccggcg tgaagtaacc gccgcgcggg ttctggtatt
    15121 tttccacttc tttgcagtag tggcgcaaca gctcggcgct aatgttatcc acctcgtcga
    15181 tatcgttacc gtatttctca aagcggttaa ttaagcgagc gcggactttt tcgccttctg
    15241 gcgttgcaaa gttggctttt aataccgaca gcaattcgtc aaaacttaaa cgctgttgat
    15301 caaaaaccat ccctttcaac gcatggagag aatcgctcag gttggcgata ccaattccct
    15361 gtacgccgga gaagttataa cgcgcgccgc catcggtaat atcgcggcct ttttccagac
    15421 aatcgctgat aaacgatgag agcagcggta caggtgccca gtcgcgatgg ccgatatcac
    15481 aaatattgct gccctcaacc atcagggtga tgtagtggct gatcttggcg cggatctgct
    15541 ccagtaaacc ttcataagtc agcgcagcat tgccttcatt ttcatgcagg cagatttcca
    15601 tcactttcag cagattaaac atcgcgatgt catgcaagcc gtaggttctg ccgggaatag
    15661 ataattccac acagcccact acggaatagt cgcgcgcatc ttccagcgaa acgccacggt
    15721 tgaggaacgc tggcaccacc acttcatcgt taaagatttg cggaataccg gtgccgaggc
    15781 gaatggtttc tgcggttttc atcaggaacg gcgtgtcgat caaggcgtta gtgcgcaccc
    15841 ctaggttcgg ttgcggtaat tgtacgcttt gataggcatc gaggcagagg aacgaaagca
    15901 cgttcaccgc gctacgtccg ttctcggtta acccgccgag cagtgcggta tagccagtcg
    15961 ggaaacctgc gaaataacgc gcgctgctgg tggagcgcaa cagcacaatg tcgttgcatt
    16021 tcacccataa agattcgagc agttctttca ggaacgccgg atcttcgccc tgggttaaag
    16081 atgcctgata gaacggcaac atatactggt cgaagcgccc taaagacagc gaactggcgt
    16141 tggattcgta ttgcagaatg atgttcatgt accagaataa ctggcacgcc tgccagaacg
    16201 tctgcggttt atgttcggcg ttatggcggg agatctccgc aatagtcagc agttcttcgc
    16261 gacgctgggc atcagtgcag cctgccgcca tcgtttccgc cagttcggcg taacgcaaaa
    16321 tgtgtttctg cgaggcttcg agcagtaaca gcgctgcctg ataaaagtga ttctccggct
    16381 gttgctgaca atgttgctgc atctgcgcca ccagctcccc cagcccgtga ttcagcaggc
    16441 gcgggtaatc aataataatg tgcccctggc ctttatccgt ctggttgatg ctgaaaatct
    16501 gcgtgctggt cgcggctttt acttcatccg tcatctgccc gttgatgaaa tctttcatcg
    16561 aacgtttttc ccagtacggg aacaactctt cgcggtagat acgtttatct tcttcgctga
    16621 tagcaaagcg gtcctgcgga cgcgtcggga attgatccag ctctttcagc agccagtaag
    16681 ggtccatttc cggcgacata atcccggcgc gtggtttcac cgtgcggtta ccggcaatca
    16741 gttcttcatc acgaatcgaa atttcaacat gttcaaggat atacgctgtt gctttcgccc
    16801 ggcgcaatat caccggttcg ccttcggttt gccgatggct ggcggtataa agcagcgcac
    16861 gctccagcga gatttcacgg gtattggcaa acagtgcagt tttgaggcga gagatacgat
    16921 tcgtcatgag gaactcctgt gggaaagcga acccgccctt gcgagcggga gaagaactta
    16981 agcggtttgc gccaggtgag cttcaatttt gctcataatg gcgtcagcgc gttttaccgc
    17041 gtcgctgatg ttgacgcgca caatggtctt gcccgcaaaa cgctcttcaa acttgatgcc
    17101 gatatctttg gtcagaatca ccatatcggc gctcgccaca tcttccgcag tcagttcgtt
    17161 ttccagacca atcgagccct gggtttctac tttcacttcc cagcctttcg ctttcgcggc
    17221 actttccagc gcctctgccg ccatataggt atgggcaaca ccagatggac atgcggttac
    17281 tgcaataatt tttgtcatgg ctcgttcctc aattaattaa tttcaaaatc cagatccagg
    17341 tcgtcttcct tctcatcagt cgaagaccct tttttacgcg ccagactttt cagcacgttc
    17401 acacaaatag ccgtcaccac tgccccgaca gccactgctg cgatataacc cagcttgcct
    17461 tcaaccaccg gcagtacaat cagaccaccc cagcctgcat agcactgcgc accgaccagc
    17521 gccgcagtta ctgcaccaca aactgaaccg accatgatgg aaggaataac acgcagcgga
    17581 tcggcagcgg cgaaaggaat cgccccttca gtaaccccaa cgcaccccat caccagcgcc
    17641 gctttaccgg tttcgcgctc ttctgcggag aaatttttac gaccaatcag cgtcgccagc
    17701 cccatcccca gcggcgggat acaaataccc acggcagcaa tggccaccac ggtataaaca
    17761 ccctgagcaa cgcaaatcag catgaaggca taggccactt tgttaaccgg accgcccata
    17821 tcgaacgcca gcatcagacc catgatcacc gccagcataa caatgctgcc ctgctgcatc
    17881 ccctgaagcc actgagtcag gctgttggtc aacgccccta caggctcgcc caagccccac
    17941 atcatgatgc ctgcggtaat cagtgtgccg atgataggaa tgatgaagat aggcatcacc
    18001 gagcgcagaa ctttatgcac cggaattttc ttcaggtaat gcaccacgat gccgccgata
    18061 atcccggcga tcagtgcacc aaagaagccc gcaccaaagc tgttaccaac ccaggcaccg
    18121 atagcgcacg gagccagcgc agaacgctct gcaatggagt aaccgatgta agcggcgagg
    18181 aaaggcacca tcagcgtcaa acctgcaacg ccgatatcaa acagtttttt cagattcgga
    18241 tcggctacgg catccggcac tgcgcctttg ccatacaaca tgacggaaac cgccagcaaa
    18301 ataccgcccg ataccacgaa gggaatcatg tgtgaaacgc ccgtcattaa atgctgacgg
    18361 gtatttttca ggatctgcac caactcattc ataagctgct cccgggcttg cgaaagccca
    18421 tgtccatatt tttgatggag gaaacaatag gcaatcgcag gcggagcaaa cagtggataa
    18481 aaaggccatt tactggactt ttgtaatatc agtacaaaaa tgcgatccgc ctcataactt
    18541 gcgataatcc ggaagcatgc cgcaaaacgg cgtcttgtga gacgcgtcgc aaattgacgg
    18601 ttacattaca tttgtccagc ttttggcaga tttgtcacat ggcaggccgt tgccgtgcgc
    18661 tcttatcctg taaacagaaa accgtaccgg gagagaggca atggccctga ttgtggaatt
    18721 tatttgtgag ctacctaacg gcgtacatgc acgtccggca agccacgttg aaacgctgtg
    18781 taatactttt tcatcacaaa ttgagtggca taacctgcgc actgaccgca agggcaacgc
    18841 caaaagcgcc cttgcgctga ttggcaccga cacgctggcg ggcgataact gccagttact
    18901 gatttccggg gccgacgaac aggaagcgca ccagcgttta agccaatggc tgcgcgatga
    18961 attcccccac tgcgatgcgc cgctggcgga agttaaatct gacgaactgg aaccactgcc
    19021 ggtttcactg accaatctga atccgcaaat tatccgcgcc cgcaccgtgt gcagcggtag
    19081 cgcaggcggc attctgacgc cgatctcttc tttagatctc aatgcgctgg gtaatcttcc
    19141 cgcagccaaa gacgttgacg ccgagcaatc cgcactggaa aacggcctga cgctggtact
    19201 gaaaaacatt gagtttcgtc tgctggatag cgacggtgcc accagcgcga ttctggaagc
    19261 gcaccgatcc ctggctggcg atacttccct gcacgaacat ttactggcag gcgtcagcgc
    19321 cggattaagc tgcgccgaag caattgttgc cagcgcgaat cacttttgcg aagagttcgc
    19381 ccgttccagc agcagctacc tgcaagaacg tgccctggac gtacgcgacg tctgcttcca
    19441 gttactccag caaatctacg gtgagcaacg cttcccggca ccgggcaaac tgacgcagcc
    19501 cgccatttgt atggctgatg aactgacccc cagccagttc ctcgaactgg ataaaaatca
    19561 cctcaaagga ttgttactga aaagcggcgg caccacctca cataccgtaa ttcttgcccg
    19621 ttcgttcaac attccgacac tggttggtgt agatattgat gcccttactc cgtggcagca
    19681 tcaaacgatt tatatcgacg gcaacgccgg ggcgattgtg gttgagccag gggaagccgt
    19741 agctcgttat tatcagcaag aagcccgcgt acaggacgcc cttcgtgagc aacagcgtgt
    19801 ctggctgacc caacaagccc gtaccgctga cggtatccgc attgaaattg ccgctaacat
    19861 cgctcactcc gtggaagcgc aggccgcatt cggcaatggt gcggaaggcg ttggtttgtt
    19921 ccgcactgaa atgctctata tggatcgcac cagtgcaccg ggcgaaagcg agctgtacaa
    19981 cattttttgt caggcgctgg agtccgccaa cggacgcagc attattgtgc gcactatgga
    20041 tattggcggt gacaaacccg ttgattatct gaacattccc gcagaggcaa acccgttcct
    20101 tggttatcgc gccgtgcgta tttatgaaga gtacgcgtcg ttatttacca cacaactacg
    20161 gtcgattctc cgtgcctccg ctcacggcag cctgaaaatc atgatcccga tgatctcttc
    20221 aatggaagag atcttatggg tgaaagaaaa actggcggaa gccaaacagc aactgcgtaa
    20281 cgaacacatt ccgtttgatg agaagatcca gctcggcatc atgctggaag tgccgtcggt
    20341 gatgttcatc atcgatcaat gctgcgaaga gattgatttc tttagtattg gtagtaatga
    20401 cctgacgcaa tatctgctgg cggtggatcg cgataacgct aaggttactc gtcactacaa
    20461 cagcctgaat ccggcattct tgcgggcgct cgattacgcc gtgcaggcgg tgcatcgcca
    20521 gggcaaatgg attggtctgt gcggtgaact gggagcgaaa ggttccgtgc tgccgttgct
    20581 ggtcggttta gggctggatg aactcagcat gagcgcaccg tcaattccgg cggcgaaagc
    20641 gcgaatggcg caacttgata gccgtgagtg ccgccagttg ctcaaccagg caatggcctg
    20701 ccgtacttcg ctggaagtgg aacatctgct ggcgcaattc cgcatgaccc aacaggacgc
    20761 accactggtc accgccgagt gcatcacgct ggaaagcgac tggcgcagca aagaagaagt
    20821 actcaaaggc atgaccgata acctgctgct ggcgggccgc tgccgctatc cgcgtaaact
    20881 ggaagccgac ttgtgggcgc gcgaggccgt tttctctacc ggtctgggct ttagttttgc
    20941 cattccccac agcaaatcag aacacattga gcagtccacc atcagcgtag cgcgtctgca
    21001 agcgccggtg cgctggggcg atgatgaagc gcaattcatc atcatgttaa ccctgaacaa
    21061 acacgctgcg ggcgatcaac atatgcgcat tttctcgcgc ctcgctcgtc gcatcatgca
    21121 cgaagaattc cgtaacgcgc tggttaacgc cgcctctgcc gacgctatcg ccagcctgct
    21181 gcaacatgaa ctggaactgt aaaaggaaac atcatggaac tgtatctgga caccgctaac
    21241 gtcgctgaag tcgaacgtct ggcacgcatt ttcccgattg ccggggtgac aactaacccg
    21301 agcattatcg ctgccagcaa ggagtccatc tgggaagtgc tgccgcgtct gcaaaaagcg
    21361 attggtgata agggcattct gtttgctcag accatgagcc gcgacgcgca ggggatggtg
    21421 gaagaagcga agcgtctgcg cgacgccatt ccgggcattg tggtgaaaat cccggtgact
    21481 tccgaaggtc tggccgccat taagatgctg aaaaaagagg gtattactac gcttggcacc
    21541 gcagtgtaca gtgctgcaca agggttactg gccgcgctgg caggggcaaa atacgttgct
    21601 ccgtatgtta accgcgtaga tgcccagggc ggagacggca ttcgtacggt tcaggagctg
    21661 caagcgctgt tagaaatgca cgcgccagaa agcatggtgc tggcagccag ctttaaaacg
    21721 ccgcgtcagg cgctggactg tttactggca ggatgtgaat ccatcaccct gcccttagat
    21781 gtagcgcaac aaatgctcaa cacccctgcg gtagagtcag ctatagagaa gttcgaacac
    21841 gactggaatg ccgcatttgg cactactcat ctctaaagga gcaattatgg accgcattat
    21901 tcaatcaccg ggtaaataca tccagggcgc tgatgtgatt aatcgtctgg gcgaatacct
    21961 gaagccgctg gcagaacgct ggttagtggt aggtgacaaa tttgttttag gttttgctca
    22021 atccactgtc gagaaaagct ttaaagatgc tggactggta gtagaaattg cgccgtttgg
    22081 cggtgaatgt tcgcaaaatg agatcgaccg tctgcgtggc atcgcggaga ctgcgcagtg
    22141 tggcgcaatt ctcggtatcg gtggcggaaa aactctcgat actgccaaag cactggcaca
    22201 tttcatgggt gttccggtag cgatcgcacc gactatcgcc tctaccgacg caccgtgcag
    22261 cgcattgtct gttatctaca ccgatgaggg tgagtttgac cgctatctgc tgttgccaaa
    22321 taacccgaat atggtcattg tcgacaccaa aatcgtcgct ggcgcacctg cacgtctgtt
    22381 agcggcgggt atcggcgatg cgctggcaac ctggtttgaa gcgcgtgcct gctctcgtag
    22441 cggcgcgacc accatggcgg gcggcaagtg cacccaggct gcgctggcac tggctgaact
    22501 gtgctacaac accctgctgg aagaaggcga aaaagcgatg cttgctgccg aacagcatgt
    22561 agtgactccg gcgctggagc gcgtgattga agcgaacacc tatttaagcg gtgtaggctt
    22621 tgaaagtggt ggtctggctg cggcgcacgc agtgcataac ggcctgaccg ctatcccgga
    22681 cgcgcatcac tattatcacg gtgaaaaagt ggcatttggt acgctgacgc agctggttct
    22741 ggaaaatgcg ccggtggagg aaatcgaaac cgtagctgcc cttagccatg cggtaggttt
    22801 gccaataact ctcgctcaac tggatattaa agaagatgtc ccggcgaaaa tgcgaattgt
    22861 ggcagaagcg gcatgtgcag aaggtgaaac cattcacaac atgcctggcg gcgcgacgcc
    22921 agatcaggtt tacgccgcac tgctggtagc tgaccagtat ggacaacgtt tcctgcaaga
    22981 gtgggaataa cctactccaa actcccggct tgtccgggag tttgaacgca aaattgcctg
    23041 atgcgctacg cttatcaggc ctacgcaatc tctgcaatat attgaatttg cgtgcttttt
    23101 aggccagata aggcgttcac gccgcatccg gcactctgtg ccagtaatct gaaactccta
    23161 cgttgttcag gagtttttta tccgcttctg gacattttct ctacagaaca atcgttatcc
    23221 cggaaggtcg aatctgtgac tcttattttc acggacgaaa cagatgaaag cttcactggt
    23281 gctggtcagc ctgctaacgg catttaccag ccactcctta aaatctcccg ccgttccacc
    23341 aaccgtggta caaatccagg ccaataccaa ccttgccatc gaagacggtg ccagacagca
    23401 aatcggcagc acgctatttt acgatccggc gtatgtgcag cttacctatc ctggcggtga
    23461 tgtcccgcaa gaacgcgggg tatgttccga tgtggtgatc cgtgcattgc gcagccagaa
    23521 ggtcgatttg cagaaactgg tgcacgaaga tatggcgaag aattttgccg agtacccgca
    23581 aaaatggcag ttaaagcgcc cggacagcaa catcgatcac cgtcgggtgc ctaatctgga
    23641 aacctggttt acccgccacg ataaaacacg ccccatcagc aagaacccca gtgactatca
    23701 agcgggcgat attgtctcct ggcgattaga taacgggctg gcacatattg gggtggtttc
    23761 agacggcttc gcccgcgacg gtacgccgct ggtgatacat aacattggcg caggtgcgca
    23821 ggaagaagat gtgctgttta gctggcgcat ggtggggcat tatcgttatt ttgttaaata
    23881 agcgatcgcc ccacatgaag aaaaaattca gatccttttt acactaacgg cttctttttt
    23941 cttacggctc accagcgcca gtgccagcac aatcagcaca ataccgctac cttcaacggc
    24001 ccccggattc tcaccgagca gccaccagga aaagagtacg ccgcaaacgg gaacggccag
    24061 tgtgcttaag ctggcaatac tggcaggcaa gtttttcagc acaaacagcc ataagctcca
    24121 cgccaacgcc gtcgccagaa tcgcactgta ggccagagcc cagaacacag tgggctgcca
    24181 gtcaatttca cgttgcggca ccagtaaagc gaccacactc atcaccagcg ccgcatagag
    24241 catctgccag gatgttaacg acaataaatc cacgcgcgga tgacgggcat acagacgttt
    24301 agcaacaatc gcgctcgccc cccaactaac accggagagg attgccagca tggcactttt
    24361 catcgaagag aaatcgagtt gccacggctg caacaccaaa aataagccga aagcggcaat
    24421 cagaatcgcg aaatattgtc cacgtcgcag gcgttcaccg agaaacaacg cggcgaaaat
    24481 caccacccag aacggcatgg tatagctcag gatcgccacc tttcccgctc cgccgctgac
    24541 cagcgcccac tgcgccaggc caaccatccc acaggtttgt aacagcgcaa tggctaaggt
    24601 gtatttaaac ggcgtcgggc gcattccgcg accacgtaat aaaaggacga tgaataaaac
    24661 gagagcgccg aaaatgcagc gtaaggcggt aaagtcgaag gcaccgatgt agctggtgac
    24721 ttgcttcatg aaaatccagc tataactcca gataagtgtg agcacaacca ggccactgat
    24781 tgccagtggg ttgctctttc ctgcggcaga catgagagat ccagtgtgta gttttcctgt
    24841 ggacactata cgccggtaaa gaaacttcag cgactgcctg aaaggcagtc gctgaagaag
    24901 gtcagattac agcaggtcga aacggtcgag gttcatcact ttcacccatg ccgccacgaa
    24961 gtctttaaca aacttctcgt gggcatcgct gctggcgtaa acttcagcca ccgcacgcag
    25021 gacagagtta gaaccaaata ccagatccgc acggctggca gtgtatttca cttcgccggt
    25081 ttcgcggtca cggccttcga acagctcttt cgattcgtcg gtcgctttcc actcgtaacg
    25141 catatccagc aagttcacga agaagtcatt gctcaatacg ccaacgcggt cagtgaagac
    25201 gccgtttttg ctgccatcga agttggcacc cagtacacgc atcccgccca ccagcgcggt
    25261 catttccggc gcggtcaggg tcagttgctg ggctttatca atcagcagtg actcggtggt
    25321 ggaaacatcc agacgagcgc gatagttacg gaaaccgtca gcaattggct ccagcagttc
    25381 gaacatctca atgtcagtct gatcctgacg cgcatcaacg cgacccggcg caaacggtac
    25441 atgaatgctc aaaccagcgg cgcttgcggc tttctcaacg ccaaccacac ctgccagcac
    25501 gatgatatcc gccagcgagg ctttaccaga ctctttctga attttctcca gaactggcag
    25561 cgcacgaacg gctgctgcgt tcacatccca gtcgcgctgc ggcattaatg ccagacgcgc
    25621 accgttggca ccaccgcgtt tgtcgccacc acggaaggta gaagcagaag cccaggccac
    25681 cgataccagc tcactaacag acagaccaga atccgcaatc gcgaatttca gatcgataat
    25741 gtcctgctcg gtcgggttgt agatcggctg cggcagcgga tcttgccaga tcagatcttc
    25801 tttcggcact tccggcccga tgtagcgaga tttcggcccc atatccctgt gcgtcagttt
    25861 gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga tcgttgagga aacgacgaga
    25921 gatcttctcg aactcaggat caaagcgcag cgtcaggtcg gtcaccagca tcgtcggttt
    25981 acgtttcttc gacggatcaa acggatccgg aataatttcc ggtgcgtcta ccgcttcgaa
    26041 ctggattgcg ccagccgggc tgcgggtctg tacccactca tacttgaaca ggttctcgaa
    26101 gaaatagttg ctccactggg tcggcgtctg ggtccagact acttccagac cagaggtaat
    26161 ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccaa cctaaacctt gttcttcaat
    26221 cggtgcagct tctggatcag gacctacatt tgatgtcgga ccagcaccgt gggttttacc
    26281 cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg tcgttcatac ccatgttgcc
    26341 gaaggtcgcg cggatagctg ctgccgcaga aagcggttcg ccgctgtgat ccgggccttc
    26401 cgggttaacg taaatcaggc ccatctcggt tgcacccagc ggtgctttcg ccagcgcttc
    26461 cggatgacgg tgagtcagcc aggctttttc atcaccccag ttaacatcca gatccggttc
    26521 ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg aagccggagt tttccagcgc
    26581 cacgttaccc gcgaggataa acaggtcggc ccaggagatt ttctgaccat atttctgttt
    26641 gattggccac aacaggcgac gtgctttatc gaggcttacg ttatccggcc aggagttcag
    26701 cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgc ccatcgattg aacggtaagt
    26761 acccgcaccg tgccaggcca tacgaataaa caaaccggcg tagctgcccc agtcggctgg
    26821 ccaccacggt tgagattctg tcagcagggc tttcagatct tttttcaggc cgtagtaatc
    26881 taatttgctg aattctttgc ggtagtcaaa gtcctcaccc agcgggttag aacgattaga
    26941 atgttggttt aacaggtcaa cacggagttg atttggccac cagtcgcgag tggttgtgcc
    27001 cgcccccgcg ctctggtcgt gaccgccctg atggaacggg catttgccag tggctgtcgt
    27061 gttatggata tcgtctgacg tgctcatcaa tgtgctcccc tctacagtgt taccgttacg
    27121 atacacagcg ttagagagaa gttataattg aattaatcca cggatgcgat agttgagatc
    27181 ttacatattg ttggttaaag agatgtagat caaattgatc gtaattaagc ttttttgatt
    27241 atgtccgaat tcggacaaat gctttataaa aaagggtttt agcttcatat ccttcaagta
    27301 atgagggaga aaacaaggct gataacctta ttttcccgcc ctcatttcga ggcagcattt
    27361 tgtgctctgt ttaaaatttg tgatcactgt gtaattttcg caaaagccac actatttata
    27421 aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact catttcagcg cggttaagcg
    27481 tatagaagtg gaaatctttc actccttcac ggcttaaaat cttcaccata tccatggcga
    27541 tattcgcgcc taccagcttg cgggtttcgg cgtcatcatc caggccgtcg aacatttgcg
    27601 ccatccacgc cggaatacgc acgttggtca tatcggcaaa tttcttcgcc tgtttaaagt
    27661 tagataccgg caaaattccc ggaataattt ccacatcaat gcccgccgat acacagcggt
    27721 cacgaaaacg caggtagctt tcgacatcga agaagaactg agtaatcgcg cggttggctc
    27781 cggcatcgac tttgcgtttc agattaagca aatccgcctg agcgcttttt gcttccgggt
    27841 gaacttccgg atacgccgcc acggagatat cgaaatctgc cacttctttt aatagcgtca
    27901 ccaggtcaga agcatacatt tctggcttac cacttcccgg cggcagatcg ccacgcagcg
    27961 ccacgatatg acgaataccg ttattccagt agtcgcgtgc aatggtgcgc aactcgtcgg
    28021 gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag accagtgcga tctttaatgc
    28081 ctttaataat gctgtgcgta cggtcgcgct cgccggagtt cgcgccatag gtcaccgata
    28141 caaacttcgg tttcaggctg ctaaggcgat cgatggagtt ccacagggtc tgctccattt
    28201 cactggtacg cggcgggaaa aactcgaacg aaacgttaat ctgcccctgg acttctgcca
    28261 ggctctgatt cagggcatcc cgctggctgg cgtgaaaaaa gctcatacct tacctcatca
    28321 atcgcttgtc gttatatgtt gtgttgtgca catctatccg tttagacgtc cagatgtaaa
    28381 gatgaaggaa aagccgaagg gcgtcaaccg aaaaatgacc aatgatggtg aggaaaaatc
    28441 agggaagatg aaaaaacttc aggatggtaa gaaaaagaaa atccggtatg caaagcaaac
    28501 cggactgcaa atattaacta accatgtact tactgcttct tgccggattc gtcgtaaacg
    28561 cttcatccgg cctaatccta tgataattca atatatttca ttagaaacca tagacccgaa
    28621 aacatgagta ccgggcatta ttaaatttct gaaattacaa caactgtgcc agccggttga
    28681 tatccgactg aatcgccccg gcggtgacgt cgcgccccgc gccaggccca cggatcacca
    28741 gcgggttgtc gcgataccag cggctttcga tggcaaagac gttatcgcac ggcagcagtg
    28801 atgccagcgg atggtcttca cgcaccgcct ccacgcccac acgcgctttg ccgttggcat
    28861 cgaaacgcgc cacgtagcgc agcaccagcc ccatttcgcg ggccgcttcc agccgttgca
    28921 ccatctgctc gttaagttca tcgccgtttt caaagaagtg gtcgatgctg ccgccttcgc
    28981 aatgggcagg caccagcgat tccacgcgca cctgatccgg ttcgatgtcg taacccgctt
    29041 cacgcgccag aatcaccagc ttgcgcatca cgtctttgcc ggagaggtcg tcacgcggat
    29101 caggctcggt taagccctgc tgccacgcct gatccaccag ctcggtaaac ggcacgctac
    29161 cgtcgaattg caggaacagc caggagagcg tgccggagaa gatcccgctg atcgacaaaa
    29221 tagtatcgcc gctgtcgatc agatcgcgca cggtgtggtt gatcggcaag cccgcaccga
    29281 cggtggcgtt gtacagccag tgacgcccgg ttttttcgaa ggcgtcgtgg atctggcgat
    29341 atttattgct gtcgctggct cctgccagtt tgttggcgct aatgacgtgg aaaccgtggc
    29401 tggcgaaatc gagatactga tcagcaagct gctggctggc ggtaacgtcc agcaccacta
    29461 aatcatcata cggatgggcg cgcatccaca ggaaaagcga ctcttcatcc tgctcgaccg
    29521 cttcatcgtt gaagaaggct aacgcgcggc tggcgtccag cccgtcataa ctcaacagac
    29581 tgcggcggct gtccaccacg cctgccagca caaattcaaa gccggtacgc gccgaaagcg
    29641 tgctctgctc gcgggcgaac agttccagcc agcgggaacc gatattgcct ttgccgaaca
    29701 ataccaggcc gatgcgtttt tctgcgcgga agacggactg atgcaggccc tgaatcaggc
    29761 tttcggtcgg gccggtgcgc agtactgcca ccaggctgat gccgtcatcg gactgccagg
    29821 taaactcgac cggctggcct ttcagttgct gccagaagcg gtggcaatgc agcgggttac
    29881 gggtgacacc cgccccgacc atcgccacca gcgccagccc ctgacgcagg cgcagttcgc
    29941 caggtaatcc cgcttcgtcg aggattttca gcgcactgtc ggccacttct gaggtgtagc
    30001 aaaattgcag caactggcga tcgttatgca cgccaaccgc cagtggacgc acctgggcac
    30061 gtttcaggat ctggtcgatc tctttatgcg ccagtttgaa atcctggctg gtgggcacct
    30121 gaaactcaat caagcagaca tcatcgtggc tggtgacaat acgcgcacca gtaccggagg
    30181 ccagcacgcg ttcaatgcgc gtggagcctt gatccggcgt gtagctacag cgcagctgca
    30241 ggtcgatttc gctaccggat actggctgta aagtacgggc gtgaagaacg ggagccgcca
    30301 gacgcgccag ttcgctggcc tcatccagac gcagcaacgg cagcagacag gcatctttca
    30361 ctttacgcgg atcggcactg tataccccgg cgacgtcgct ccagatggtt acacgagaaa
    30421 cacccgccag cgcaccgatt tgtgtcgcgg aatagtcgga accgttacgc cccagcagca
    30481 ccgtttcacc ggcgttgtta cggctgataa acccggtcac caccagacgt ttacccggat
    30541 gttgtaccag tagctgttgc agcaacgggt aagaaagccc ttcatcaacc tgcggttgcg
    30601 cagcgcgttc agcgcgtaaa aactcacggg catcaagcca ggccgctggc agcccttgtt
    30661 gattaagtac cgcagacatc aggcgcgccg accacacttc accgtggccc accacttccg
    30721 catacactgc gtcgttaata ccgctgtcga gcagcgccgc caggcgctca agatcgctga
    30781 caaaagcgct aatgaggcta tcggcttctt cggcgggtag caggccgcta atcagatcgc
    30841 actgatagcg acgcagcgtt tgttgaacct gatgcgcaga gagacgatcg gtctggctta
    30901 gtttcaacca gttaatcaac tggttagtgg tgctaccggc ggcggaaacc accatcatat
    30961 cgtcaggctg agagtactcc gccataatgc ccgcgacacg caaataacac ttcacatcag
    31021 ccagactact gccaccaaat ttatgcagct gacgaccttt cgcccctgcc tgcgcaatca
    31081 cactcatttt taccccttgt ttgcagcccg gaagccattt tccaggtcgg caattaaatc
    31141 ttcgccatct tcaataccgg tggagatacg cagcagcgtc tcggagatcc cggcggcagc
    31201 acgcgcttct ggtgccatgc ctgcatgtgt catggttgcg gcgtgagaga ttaaactttc
    31261 cactccccct aatgattccg ccagcgtaaa caacgacagc ccgcccagga aacgacgcag
    31321 cgtctgctca tcgccatcca gttcaaaact caacattgcg ccaaagcctt tttgctgacg
    31381 cgcggcaatt tcatgcccct gattttccgg cagcgacggg tgatacagtt ttttcaccaa
    31441 cggctgggtt tgcagatatt tcacaatcgc ctgcgcgttg cgctgcgcca gctccatacg
    31501 cggcaccagc gttcgcaacc cgcgtagcag cagatagctg tcaaacgcgc cgcccgtcac
    31561 gccaatattg tttgcccacc aggcaagttc agtgacaacg tccgggtctt tagcaatcac
    31621 cacgccggcc accacgtctg agtgaccgtt cagatatttc gtacatgaat gcaacaccag
    31681 atcggcaccc aatgccagcg gattttgtaa tgccgggctt aagaaggtgt tatccaccac
    31741 gctcaccgcc ccgacttccc ttgccagatg gcagattttc gcaatatcca cgacgcgtaa
    31801 caatggatta cttgggcttt ctaccagtac cagtttgggt ttttctgcca gcgctgcccg
    31861 taatgcctgt tcatcgcctt gatcaacaaa caacacgcga tagcaaccgc gtttcgccag
    31921 gctgtcgaac aggcgatagc taccgccgta gcagtcgtgc ggcgcaacca gcagatcgcc
    31981 aggtttcaaa aagacggtcg ttaccaggtg aatcgcggac atgccggtat tagtaagtac
    32041 tgcaccagca ccgccttcca gttctgccag cgcacgctga accacatcgc gcgttgggtt
    32101 gccgcgacgc gagtaatcat gcgcgcgcgg ttcattaaat ccggtaaagt tataggtgct
    32161 ggaaagatgg atgggaggga caacgcaacc atactgttcg tcgtcattta acccgctacg
    32221 cactgcgatg gtggcctgtt tacgcgtcat gtgatgaagt tccctgggct tggtcggtga
    32281 aatgtcaggc accagagtaa acattgtgtt aatggacgtc aatacatctg gacatctaaa
    32341 cttctttgcg tatagattga gcaaatccca aatagccgtt aaaattatat gcattatcac
    32401 gccgacaggt gcattacacg atgtcacggt aacgcctgta cggtaaacta tgcgggttta
    32461 cggtcagtac ccacataaat tgtgtggtct ggtctcaatt tattgacgaa gaggattaag
    32521 tatctcatgg ctgaatggag cggcgaatat atcagcccat acgctgagca cggcaagaag
    32581 agtgaacaag tcaaaaagat tacggtttcc attcctctta aggtgttaaa aatcctcacc
    32641 gatgaacgca cgcgtcgtca ggtgaacaac ctgcgtcacg ctaccaacag cgagctgctg
    32701 tgcgaagcgt ttctgcatgc ctttaccggg caacctttgc cggatgatgc cgatctgcgt
    32761 aaagagcgca gcgacgaaat cccggaagcg gcaaaagaga tcatgcgtga gatggggatt
    32821 aacccggaga cgtgggaata ctaaagcgga aaagagcggc gcggagttaa ccagccgctt
    32881 ttttcataca aggacgttta acgatgaaaa tcaggctact gatcctcagc ctgctggttt
    32941 ctgtgcctgc ctttgcctgg cagccgcaaa ctggcgacat catctttcaa atctctcgct
    33001 catcgcaaag taaagcgatc caactggcga cccattccga ttacagccac accggtatgc
    33061 tggtgatgcg taacaaaaag ccctacgttt ttgaagcggt cggcccggtg aaatacaccc
    33121 cgctcaagca gtggattgcc catggtgaaa agggcaaata cgttgttcgc cgcgttgaag
    33181 gcggactcag tgttgaacaa cagcaaaaac tggcgcaaac ggcgaaacgt tatctcggca
    33241 aaccgtatga cttcagcttc tcatggagcg acgatcgcca gtactgttcg gaagtggtgt
    33301 ggaaggttta tcagaatgcg ctgggaatgc gcgtgggcga acagcagaag ttgaaagagt
    33361 tcgacctgag cagcccacaa gttcaggcaa agctcaaaga acgttacggc aagaatattc
    33421 cgcttgagga aacggtggtc tcgccacaag ccgttttcga tgcgccacaa ctcaccacgg
    33481 tcgccaaaga atggccgctg ttttcgtggt aatggatgat aaaaaaaata attcccgccg
    33541 tagtctgttt gttgctttgt agttgcacga cctttccgca agctgtcgct ccggtaaacg
    33601 gtgatgcctc caattagttg aattaaggat ataatactcg cttttaaggt tgtttcatgg
    33661 tcacagttaa ccttcattgt ccccgttgcc agtccgttca ggtttaccgt cacgggcaga
    33721 accccaaaag acatgatcga ttccgcggcc gtgactgtca ccgggtattt cagctcagtt
    33781 actgttacga gccccgtaaa ccgggagtta aagaccaaat cgctgaaatg gctttcaacg
    33841 gtgccggggt tcgctatact gcccgtacgc tgaaaatcgg cattaataca gtcatccgaa
    33901 ctttaaaaaa ctctcgccac gaagaataac gtcatccccg gtcgctcatg ctgatgtggc
    33961 actcatctgc gaacttagtc agcaatggag ctttgtcggc agtaaagcgc gacagcactg
    34021 gctctggtac gtgtataaca cgaaaactgg tggtgttctg gcctacactt ttggcccccg
    34081 gactgatgaa acctgtcgtg aattgctggc gctgcttacc cttttaccat cggcatgttg
    34141 accactgacg aatggggtag ttacgctaga gagttaccaa aggagaagca tctgactggt
    34201 aagattttta ctcagcacat tgagcgtaac aatctgaccc tgagaacccg aatcaaacgt
    34261 ctggcccgca gaaccatctg tttctcgcgt tctgtagaac ttcatgaaaa agtcatcggt
    34321 gcttttatcg aaaatatatg ttctactaat tggagtcatc acccttctga tgagctttcc
    34381 ttggatgatt accagactga atacacagcc aaagattttt acctcattct gctctttttt
    34441 ctcaaatgac atatattata ttttacatca tattcatagg catatgacat tcccccgccc
    34501 ccca
//
LOCUS       DAZGBC010000034        33702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGBC010000034 DAZGBC010000000
VERSION     DAZGBC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.034"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            19..255
                     /gene="ydhI"
                     /locus_tag="ACHK1J_001669"
     CDS             19..255
                     /gene="ydhI"
                     /locus_tag="ACHK1J_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO7910957.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            258..1115
                     /gene="ydhJ"
                     /locus_tag="ACHK1J_001670"
     CDS             258..1115
                     /gene="ydhJ"
                     /locus_tag="ACHK1J_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO7910958.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVFSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1115..3127
                     /gene="ydhK"
                     /locus_tag="ACHK1J_001671"
     CDS             1115..3127
                     /gene="ydhK"
                     /locus_tag="ACHK1J_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7910959.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3128..3649)
                     /gene="sodC"
                     /locus_tag="ACHK1J_001672"
     CDS             complement(3128..3649)
                     /gene="sodC"
                     /locus_tag="ACHK1J_001672"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO7910960.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3730..4626)
                     /gene="ydhF"
                     /locus_tag="ACHK1J_001673"
     CDS             complement(3730..4626)
                     /gene="ydhF"
                     /locus_tag="ACHK1J_001673"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO7910961.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4675..4914)
                     /gene="ydhL"
                     /locus_tag="ACHK1J_001674"
     CDS             complement(4675..4914)
                     /gene="ydhL"
                     /locus_tag="ACHK1J_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO7910962.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5017..5616
                     /gene="nemR"
                     /locus_tag="ACHK1J_001675"
     CDS             5017..5616
                     /gene="nemR"
                     /locus_tag="ACHK1J_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO7910963.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRNSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATSAV"
     gene            5654..6751
                     /gene="nemA"
                     /locus_tag="ACHK1J_001676"
     CDS             5654..6751
                     /gene="nemA"
                     /locus_tag="ACHK1J_001676"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO7910964.1"
                     /translation="MSSEKLYSPLQVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSQEQIAAWKKITAGVHAENGHIAVQL
                     WHTGRISHASLQPDGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRTRFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     TRLQRKAGLNPQRAESFYGGGAEGYTDYPTL"
     gene            6832..7239
                     /gene="gloA"
                     /locus_tag="ACHK1J_001677"
     CDS             6832..7239
                     /gene="gloA"
                     /locus_tag="ACHK1J_001677"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO7910965.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7342..7989
                     /gene="rnt"
                     /locus_tag="ACHK1J_001678"
     CDS             7342..7989
                     /gene="rnt"
                     /locus_tag="ACHK1J_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO7910966.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8082..12698
                     /gene="lhr"
                     /locus_tag="ACHK1J_001679"
     CDS             8082..12698
                     /gene="lhr"
                     /locus_tag="ACHK1J_001679"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO7910967.1"
                     /translation="MADNPNLSSPLPDVFSPATRDWFLSAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDVAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRATPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILSARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRTSQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPAMISGQERLACMDDAARLRDALGVQLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12749..13096)
                     /gene="grxD"
                     /locus_tag="ACHK1J_001680"
     CDS             complement(12749..13096)
                     /gene="grxD"
                     /locus_tag="ACHK1J_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO7910968.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13431..14258
                     /gene="mepH"
                     /locus_tag="ACHK1J_001681"
     CDS             13431..14258
                     /gene="mepH"
                     /locus_tag="ACHK1J_001681"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO7910969.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14386..14967
                     /gene="sodB"
                     /locus_tag="ACHK1J_001682"
     CDS             14386..14967
                     /gene="sodB"
                     /locus_tag="ACHK1J_001682"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO7910970.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15113..16282)
                     /gene="ydhP"
                     /locus_tag="ACHK1J_001683"
     CDS             complement(15113..16282)
                     /gene="ydhP"
                     /locus_tag="ACHK1J_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7910971.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16448..16537)
                     /gene="cydH"
                     /locus_tag="ACHK1J_001684"
                     /gene_synonym="ynhF"
     CDS             complement(16448..16537)
                     /gene="cydH"
                     /locus_tag="ACHK1J_001684"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO7910972.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16836..17861
                     /gene="purR"
                     /locus_tag="ACHK1J_001685"
     CDS             16836..17861
                     /gene="purR"
                     /locus_tag="ACHK1J_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO7910973.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17858..18790)
                     /gene="punR"
                     /locus_tag="ACHK1J_001686"
     CDS             complement(17858..18790)
                     /gene="punR"
                     /locus_tag="ACHK1J_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO7910974.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18903..20114
                     /gene="punC"
                     /locus_tag="ACHK1J_001687"
     CDS             18903..20114
                     /gene="punC"
                     /locus_tag="ACHK1J_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO7910975.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20405..21553
                     /gene="cfa"
                     /locus_tag="ACHK1J_001688"
     CDS             20405..21553
                     /gene="cfa"
                     /locus_tag="ACHK1J_001688"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO7910976.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21593..22234)
                     /gene="ribE"
                     /locus_tag="ACHK1J_001689"
     CDS             complement(21593..22234)
                     /gene="ribE"
                     /locus_tag="ACHK1J_001689"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO7910977.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22449..23822
                     /gene="mdtK"
                     /locus_tag="ACHK1J_001690"
     CDS             22449..23822
                     /gene="mdtK"
                     /locus_tag="ACHK1J_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO7910978.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(23863..25119)
                     /gene="ydhQ"
                     /locus_tag="ACHK1J_001691"
     CDS             complement(23863..25119)
                     /gene="ydhQ"
                     /locus_tag="ACHK1J_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO7910979.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIIENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25427..25503
                     /locus_tag="ACHK1J_001692"
     tRNA            25427..25503
                     /locus_tag="ACHK1J_001692"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25461..25463,aa:Val,seq:gac)
     gene            25508..25584
                     /locus_tag="ACHK1J_001693"
     tRNA            25508..25584
                     /locus_tag="ACHK1J_001693"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25542..25544,aa:Val,seq:gac)
     gene            25692..25997
                     /gene="ydhR"
                     /locus_tag="ACHK1J_001694"
     CDS             25692..25997
                     /gene="ydhR"
                     /locus_tag="ACHK1J_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO7910980.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26123..27727
                     /gene="ydhS"
                     /locus_tag="ACHK1J_001695"
     CDS             26123..27727
                     /gene="ydhS"
                     /locus_tag="ACHK1J_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO7910981.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADVAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEAIRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27739..28551)
                     /gene="ydhT"
                     /locus_tag="ACHK1J_001696"
     CDS             complement(27739..28551)
                     /gene="ydhT"
                     /locus_tag="ACHK1J_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO7910982.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARIAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(28555..29340)
                     /gene="phsC"
                     /locus_tag="ACHK1J_001697"
     CDS             complement(28555..29340)
                     /gene="phsC"
                     /locus_tag="ACHK1J_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO7910983.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29337..30005)
                     /gene="ydhX"
                     /locus_tag="ACHK1J_001698"
     CDS             complement(29337..30005)
                     /gene="ydhX"
                     /locus_tag="ACHK1J_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7910984.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30069..30707)
                     /gene="ydhW"
                     /locus_tag="ACHK1J_001699"
     CDS             complement(30069..30707)
                     /gene="ydhW"
                     /locus_tag="ACHK1J_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO7910985.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30720..32822)
                     /gene="ydhV"
                     /locus_tag="ACHK1J_001700"
     CDS             complement(30720..32822)
                     /gene="ydhV"
                     /locus_tag="ACHK1J_001700"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO7910986.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(32843..33469)
                     /gene="ydhY"
                     /locus_tag="ACHK1J_001701"
     CDS             complement(32843..33469)
                     /gene="ydhY"
                     /locus_tag="ACHK1J_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO7910987.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLTGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8085 a         8878 c         8301 g         8438 t
ORIGIN      
        1 cgtaaaggaa tctactttgt gaagtttatg ctcaatgcaa caggattgcc cttacaagat
       61 ctggtgttcg gtgcgtccgt ctactttcct ccgtttttca aagcattcgc gtttggattc
      121 gtcatctggc ttgtcgtaca ccgcctgctt cgtggctgga tctacgccgg tgacatctgg
      181 catcccttgt taatggattt atcgctgttt gcgatttgcg tttgccttgc tctggcaata
      241 ctgattgcgt ggtaactatg tcaattaaaa caattaagta tttctcaaca atcattgtag
      301 cggtagttgc ggttcttgcc ggatggtggc tgtggaatta ttacatgcaa tctccgtgga
      361 cacgcgacgg gaaaatacgc gcagaacagg tttctatcac cccacaggtg tcgggacgta
      421 ttgttgagct gaatataaaa gacaaccagc tggttaacgc gggggatctt ctgctcacga
      481 tcgataaaac gccctttcag atcgccgagc tgaacgcaca ggcacagctg gcgaaagcgc
      541 aatctgacct cgcaaaagcc aataacgaag cgaatcgtcg tcgtcatctc tcacaaaatt
      601 ttatttctgc cgaagaactc gataccgcta acctcaatgt taaagcgatg caggccagtg
      661 ttgatgccgc acaggcgacg ctgaaacaga cacaatggca actggcgcaa acggaaattc
      721 gcgccccggt gagtggatgg gtgactaacc tcactacccg catcggcgat tacgctgaca
      781 ccgggaaacc gctgtttgcc cttgtcgaca gccactcgtt ttacgtcatt ggttattttg
      841 aagaaaccaa gttgcgccat atccgcgaag gtgcaccagc acaaattacc ctctatagtg
      901 ataacaaaac gttacagggt cacgttttca gtatcggtcg ggcgatttat gatcagagcg
      961 ttgaaagtga ctcaagcctt attccggatg tgaaacctaa cgtcccctgg gttcgtctcg
     1021 cccaacgcgt tcccgtccgc tttgcgctgg ataaagttcc aggcgatgtc acgctggtgt
     1081 cgggcaccac ctgtagcatt gccgtaggtc aataatgaac gcatcgtcat ggtccttgcg
     1141 caatttgccc tggttcaggg ccacgctggc gcaatggcgt tatgcgttac gcaataccat
     1201 tgctatgtgt ctggcgctga cggttgccta ttatttgaat ctggatgaac cctattgggc
     1261 gatgacctcg gctgcagtgg ttagctttcc caccgttggc ggtgtcatca gcaaaagcct
     1321 cggacgcatc gctggcagtt tactgggagc cattgcggca ctgcttcttg ccggacatac
     1381 gctcaatgag ccgtggtttt ttctattgag catgtcggcg tggcttggct tttgtacctg
     1441 ggcctgtgcg cacttcacga ataacgtcgc gtatgcattt caactggcgg gctacacggc
     1501 tgccatcatc gcctttccga tggttaatat tactgaggcc agccagctgt gggatatcgc
     1561 tcaggcgcgc gtttgcgagg tgattgtcgg tattttgtgc ggcggcatga tgatgatgat
     1621 cctgccgagc agttccgatg ctactgccct tttaaccgca ttgaaaaaca tgcacgcccg
     1681 attactggaa catgccagtt tactctggca gcctgaaaca accgatgcca ttcgtgcagc
     1741 acatgaaggg gtgattgggc agatactgac catgaatttg ctgcgtatcc aggctttctg
     1801 gagccactat cgttttcgcc agcaaaacgc gcgccttaat gcgctgctcc accagcaatt
     1861 acgtatgacc agtgtcatct ccagcctgcg acgtatgttg ctcaactggc cctcaccgcc
     1921 aggtgccaca cgagaaattc tcgaacagtt gctgacggcg ctcgccagtt cgcaaacaga
     1981 tgtttacacc gtcgcacgta ttatcgcccc gctacgcccg accaacgtcg ccgactatcg
     2041 gcacgtcgcc ttctggcagc gactacgtta tttttgccgc ctttatctgc aaagtagtca
     2101 ggaattacat cgtctgcaaa gcggtgtaga tgatcatacc agactcccac ggacatccgg
     2161 cctggctcgt cataccgata acgccgaagc tatgtggagc gggctgcgta cattttgtac
     2221 gttgatgatg attggcgcat ggagtattgc ttcgcaatgg gatgccggtg ccaatgcatt
     2281 aacgctggca gcaattagct gcgtactcta ctccgccgtc gcagcaccgt ttaagtcgtt
     2341 gtcactgctg atgcgcacgc tggtgttact ttctctattc agctttgtgg tcaaatttgg
     2401 cctgatggtc cagattagtg atctgtggca atttttgctg tttctctttc cactgctggc
     2461 gacgatgcag cttcttaaat tgcagatgcc aaaatttgcc gcactgtggg ggcaactgat
     2521 tgtttttatg ggttcgttta tcgctgtcac taatcccccg gtgtatgatt ttgctgattt
     2581 tcttaacgat aatctggcga aaatcgttgg cgtcgcgttg gcgtggttag cgttcgccat
     2641 tctgcgtcca ggatcggatg ctcgtaaaag ccgtcgccat attcgcgcgc tgcgtcggga
     2701 ttttgtcgat caactaagcc gccatccaac actgagtgaa agcgaatttg aatcgctcac
     2761 ttatcatcac gtcagtcagt tgagtaacag ccaggatgcg ctggctcgcc gttggttatt
     2821 acgctggggt gtagtgctgc tgaactgttc tcatgttgtc tggcaattac gcgactggga
     2881 atcgcgttcc gatccgttat cgcgagtacg ggataactgt atttcactgt tgcggggagt
     2941 gatgagtgag cgtggcgttc agcaaaaatc actggcggcc acacttgaag aattacagcg
     3001 gatttgcgac agccttgccc gtcatcatca acctgccgcc cgtgagctgg cggcaattgt
     3061 ctggcggctg tactgctcgc tttcgcaact tgagcaagca ccaccgcaag gtacgctggc
     3121 ctcttaatta cttaattaca ccacaggcat agcgttcacc gccaccgccc agcggtttag
     3181 gttgatcgga catattatcg ccgccaacgt ggaccatcag cgctttgtct ttgatttcat
     3241 ccagtgattt cagacgaggc gcgatgacgg catcggtagc tttgccgtca ttattgacta
     3301 ccagtgcagg cagatcgcct aaatgcccgg caccttctgg cccttcatgt ttgccggtat
     3361 tttgtggatc aagatgcccg cctgcggatt ccgcggcgct ggctttgcca tctttggtgg
     3421 ctggctggca gcttcctttg gcatgaatat ggaagccatg ttcaccgggg ggtaatgctt
     3481 tcagatcggg cgaaaactcc agacctttat cggtttcagt aatggtgacg ctaccaattg
     3541 actgcccaac cccttgcgac gtgacgaggt tcatctcgac tttttcactg gcagcttgtg
     3601 cgccggttgc aacaaccagc gccagaatag ccagactaaa acgtttcata ggacctccgt
     3661 tcattgtgca ttcctgaata agtgtaaacc agtgacacat ttttgaacgg cggctattcc
     3721 taaaagtgct tacggtacgt cgtaccccag tgccgcttta cggatacgaa accattgttg
     3781 acgggtcatt ttcagtgttt ctgcttcgac agctgcccgt acgcgctcaa ttttacctga
     3841 gccgataatt ggcagcggct gcgacggtaa acgtaatacc caggcgtaaa ccacctgttc
     3901 aatcgagccc gcgtttaact cctccgccac cacagccagt tcatcacgta gcggctggaa
     3961 ataatcatca ttaaacagac gaccaccacc aaggcaggac cacgccatcg gacgaacacg
     4021 cagttgttgc agttggtcga gcgtgccatc cagcagtaac ggctgatgta ccggggatat
     4081 ttccacctga ttagtggcaa gggtaaacgg caggcgtgat tgcaacaggg caaattgcgc
     4141 gggcgtgaag ttggatacgc caaaatgacg cactttgccg ctctgatgca gatgtttgaa
     4201 cgcgtccgcc acttcatcgg catccattaa cgggtccggg cggtggatta acagcagatc
     4261 cagatgatcg gtcgcgagat taatcagcga ctgttcggcg cttttaataa tatggtcgcg
     4321 gtcagtgatg taatggccaa tgacgttttc ttcacgcgca gtcgtagcaa tgccacattt
     4381 gctgacgatt tccatccgtt cacgcagatg tggggccagt ttcagcgcct cgccaaacgc
     4441 cgcttcgcac tgatagccac cataaatatc agcatggtcc acggtggtca cgccgagatc
     4501 cagatgctct tcaataaaac tgaccagctg gcgggcggac atattccagt ccatcaatcg
     4561 ccagtagccc atcacaaaac gggaaaactc cgggccttgc ggcgcaatag taatacgctg
     4621 aaccatattc gcttcctctt atcagatatg agaggagtat acgcaagatt aggctcaaaa
     4681 gagtgatggt tgctccggtt cgtctgatga cgctggctta tttgcacgta atttgcgcat
     4741 taatcgctgc cgacaaaggc gcagcacctc ttgtttttcg ccatcgctca ttttattcca
     4801 gttaaaacgc tcatcccggc ttcgaaaaca gccgcgacaa aacccgcgtt cgtcagactg
     4861 gcaaattccc cggcacgggc tctggacggg aaagaactct aattgctccg ccacttcgcc
     4921 ctcctcagat aagattatta ccattattga agctgttaat gtccaaagta gcaactttgc
     4981 ttgcgctaga ccgactggtc tactacactc caacacatga acaaacacac cgaacatgat
     5041 actcgtgaac atctcctggc gacgggcgag caactttgcc tgcaacgtgg attcaccggg
     5101 atggggctaa gcgaattact aaaaaccgct gaagtgccga aagggtcctt ctatcactac
     5161 tttcgctcta aagaagcgtt tggcgttgcc atgcttgagc gccattacgc cgcatatcac
     5221 cagcgactga cggagttgct gcaatccgga gaaggtaact accgcgaccg catactggct
     5281 tattaccagc aaacactgaa ccagttttgc caacatggaa ccatcagtgg ttgcctgaca
     5341 gtaaaactct ctgccgaagt gtgcgatctg tcagaagata tgcgcagcgc aatggataaa
     5401 ggtgctcgcg gcgtgatcgc cctgctctct caggcgctgg aaaatggtcg taatagccat
     5461 tgtttaacct tttgcggcga accgctacaa caggcgcaag tgctttacgc gctgtggctg
     5521 ggtgcgaatc tgcaggccaa aatttcgcgc agtttcgagc cacttgaaaa cgcgctggca
     5581 catgtaaaaa ccattattgc gacgtctgcc gtttagcagg cattttttta tcaccagacg
     5641 accgggagcc tttatgtcat ctgaaaaact gtattcccca ctgcaagtgg gcgcgatcac
     5701 ggcggcaaac cgtattttta tggcaccgct gacgcgtctg cgcagtattg aaccgggtga
     5761 catccctacc ccgttgatgg cggaatacta tcgccaacgt gccagtgctg gtttgattat
     5821 tagcgaagcc acgcaaattt ctgcccaggc aaaagggtat gctggtgcgc ctggcatcca
     5881 tagccaggag caaattgccg cgtggaaaaa aatcaccgct ggcgttcatg ctgaaaatgg
     5941 tcatattgcc gtgcaactgt ggcataccgg acgtatttct cacgccagcc tgcaacctga
     6001 cggtcaggca ccggtagcac cttcagcact tagcgcggga acacgcactt ctctgcgcga
     6061 tgaaaatggt caggcgatcc gtgttgaaac atccatgccg cgtgcgcttg aactggaaga
     6121 gattcctggt atcgtcaacg atttccgcca ggccatagcc aacgcccgcg aagccggttt
     6181 tgatctggtc gagctccact ctgctcacgg ttatttgctg catcagtttc tttcgccttc
     6241 ttcgaaccat cgtaccgatc agtacggcgg tagcgtggaa aatcgcgcac gtctggtgct
     6301 ggaagtggtc gatgcaggca ttgaagaatg gggtgccgat cgcattggca ttcgcgtttc
     6361 gccaatcggt actttccaga acacggataa tggcccaaat gaagaagccg atgcgctgta
     6421 cctgattgaa caactgggta aacgcggcat tgcttatctg catatgtcag aaccggattg
     6481 ggcgggaggt gaaccgtata ctgatgcgtt ccgcgaaaaa gtacgcaccc gtttccacgg
     6541 tccgattatc ggcgcaggtg catacacagt agaaaaagct gaaacgctga tcggcaaagg
     6601 gttaattgat gcggtggcat ttggtcgtga ctggattgcg aaccctgatc tggttacccg
     6661 cttgcagcgc aaagccggac ttaacccaca gcgtgctgaa agcttctatg gcggtggtgc
     6721 ggaaggctac actgattacc cgacgctgta atccaacatt gcgagcggcg caaagccgcc
     6781 gctatactaa aacaacattt tgaatctgtt agccattttg aggatataaa gatgcgtctt
     6841 cttcatacca tgctacgcgt tggcgatttg caacgctcca tcgattttta taccaaagtt
     6901 ctgggcatga aactgctgcg taccagcgaa aacccggaat acaaatactc actggcgttt
     6961 gttggctacg gcccggaaac cgaagaagcg gtgattgaac tgacctacaa ctggggcgtg
     7021 gataaatacg aactcggcac tgcttatggt cacatcgcgc ttagcgtaga taatgccgct
     7081 gaagcgtgcg aaaaaatccg tcaaaacggg ggtaacgtga cccgtgaagc gggtccggta
     7141 aaaggcggta ctacggttat cgcatttgtg gaagatccgg acggttacaa aattgagtta
     7201 atcgaagaga aagacgccgg tcgcggtctg ggcaactaat ctcctgccgg gcgcgaactc
     7261 atcgcgcccg catctttact gcatcgacaa gtaatatttg tcataatgcg cgctgcaatt
     7321 tatccgtatt aagagaatca gatgtccgat aacgctcaac ttaccggtct gtgcgaccgt
     7381 tttcgtggtt tttatcctgt tgtgatcgat gttgaaacag ccggatttaa cgccaaaacc
     7441 gatgcgctgc ttgagattgc cgccatcacc ctgaaaatgg atgaacaagg ctggctgatg
     7501 ccggacacca cattacattt ccacgtcgaa ccatttgtcg gcgcaaattt gcaaccagaa
     7561 gcccttgcct tcaacggcat tgacccgaat gatcccgatc gcggcgcggt cagcgaatac
     7621 gaggcgctgc acgaaatttt taaagttgta cgtaaaggta ttaaagcgag cggctgtaac
     7681 cgcgccatta tggtggcgca caacgccaat ttcgatcaca gctttatgat ggccgccgct
     7741 gaacgcgcct cactgaaacg taacccgttc caccctttcg ccacttttga cactgctgca
     7801 ctggccgggc tggcactcgg acaaaccgta ttgtcaaagg cttgccagac cgctggcatg
     7861 gacttcgaca gcacccaagc tcactccgcg ctgtacgaca ccgaacgcac tgcggtgctg
     7921 ttttgtgaaa tcgtcaaccg ctggaaacgt ctgggaggct ggccgctacc tgccgccgaa
     7981 gaggtgtaat cgagtcgatg cctgatgaca tgcaatgatt caggcatcta tagtgaggct
     8041 attccacgca tcatgcatga tattcacggg gaatagcgtt aatggcagat aatccaaacc
     8101 tttcatcgcc cctgccggac gtgttttcac cggcgacccg cgactggttt cttagcgcct
     8161 ttaaacagcc gaccgctgtc cagtcgcaaa cctggcatgt ggcggcgcga ggcgaacatg
     8221 cgctggtgat tgcaccgacc ggctccggga aaacgctggc agcatttctc tacgccctcg
     8281 atcggctctt ccgcgaaggc ggcgaagata cccgcgaggc gcataagcgt aaaacctcac
     8341 gcattctcta tatttcaccg ataaaagccc tgggcaccga cgttcagcgc aacttgcaga
     8401 tcccgttgaa gggtattgcc gatgaacggc ggcggcgcgg cgaaacggaa gtcaatcttc
     8461 gcgtggggat ccgtactggc gatacgcctg cacaggaacg cagcaaactc acccgtaatc
     8521 caccggatat tctgatcacc acacccgaat cactctatct gatgctgacc tcccgcgcgc
     8581 gcgaaacgct acgcggcgtc gaaacggtaa ttattgatga agtccacgcg gtagcgggca
     8641 gtaaacgtgg tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg ctccacacct
     8701 cagcacagcg aattggcctt tctgccactg tgcgctcagc cagcgatgtg gcagcatttc
     8761 ttggcggcga tcgcccggtt acggtagtca acccgcccgc aatgcgccat ccgcagatac
     8821 ggattgtcgt accggtcgcc aatatggatg atgtctcatc ggtcgccagc ggcactggcg
     8881 aagacagcca tgccggccgg gaaggctcca tctggccata tattgaaacg ggtatccttg
     8941 atgaagtgtt gcgccatcgc tcgaccattg tctttactaa ttcgcggggg ctggcggaaa
     9001 aactgacggc acgattaaat gagctttacg ccgcacgctt acagcgttcc ccttctatcg
     9061 ccgttgatgt ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt gtgcaaagta
     9121 gcgacatttt tattgcccgc tcacaccacg gctccgtctc taaagaacaa cgagcaatca
     9181 ccgaacaggc gctgaaatcg ggtgaattac gctgcgtggt cgcaacctcc agtctcgaac
     9241 tggggattga tatgggcgct gtggatctgg tgattcaagt ggcaacgccg ctttctgttg
     9301 ccagtgggtt acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta tctaaagggc
     9361 tgtttttccc ccgaacccgg cgtgacttag tcgattccgc agtcattgta gagtgtatgt
     9421 tcgcaggcag gctggaaaac ctgacaccac cgcataatcc actcgacgtc cttgcgcagc
     9481 acaccgttgc cgccgcggcg atggatgcat tacaggtaga cgaatggtac tcccgcgtac
     9541 gccgtgccac accgtggaaa gatctgccaa gacgtgtttt tgacgccacg ctggatatgc
     9601 tttccgggcg ctatccctct ggcgattttt ctgcttttcg accaaaactg gtctggaacc
     9661 gggagaccgg gatattgtcc gcccgacctg gcgctcagct gttggcggtt accagcggtg
     9721 gcaccattcc ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa gaacaggccg
     9781 gttcgcggcg ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg gtgaacgata
     9841 ttatcactct cggcgctacc tcatggcgga tccagcaaat cacccgcgat caggtgattg
     9901 tgactcctgc tccgggtcgt tctgcccggc ttcccttttg gcgtggtgaa ggtaacggac
     9961 gtccggctga attaggcgag atgatcggcg attttcttca tttactggcg gatggcgcgt
    10021 tcttttccgg gactattccc ccgtggctgg cagaagaaaa tacgatcgcc aatattcagg
    10081 ggttgattga cgagcagcgc aacgcgacgg gtattgttcc ggggagtcgt catctggtcc
    10141 tcgaacggtg ccgtgatgaa attggcgact ggcgtattat tttgcactct ccctatggaa
    10201 gacgggtgca tgaaccctgg gcgctggcga ttgccgggcg aatacatgcg ctatggggcg
    10261 ctgacgcgtc tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct gacactgatg
    10321 gtaaattgcc cgacgccgcg atttttttgt ttgaaccaga aaagttgctg caaattgtcc
    10381 gcgaggcggt aggcagctca gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg
    10441 cgttattaat gccgggccgc acgccgggcc atcgcacccc gctctggcaa caacggctgc
    10501 gcaccagtca gttgctggaa atcgctcagg gatatccgga ttttccggtc attctcgaaa
    10561 ccctacgcga atgtctgcaa gatgtttatg atcttcccgc actggaacgt ttgatgcgtc
    10621 gcctgaacgg tggcgaaatt caaatatccg atgtaacgac tactacgccc tcgcctttcg
    10681 ccaccagttt attgttcggc tatgtcgcgg aatttatgta ccagagcgac gccccgctgg
    10741 cagagcgccg ggcatccgta ctgtcactgg acagcgagtt actgcgcaat ctactcgggc
    10801 aggtcgatcc gggggaatta ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc
    10861 aacgactggc tcctggcaga agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg
    10921 aactggggcc aatgaccgtt gaagacctgg cgcaacggca tacaggcagc agtgaagaga
    10981 ttgcgtcgta tctggaaaat cttcttacag taaaacgcat cttcccggcg atgattagcg
    11041 gacaggagcg tcttgcctgt atggatgatg ccgccaggct gcgcgatgcc ctcggcgtac
    11101 aactaccaga gtctctgcca gagatttatt tgcatagagt cagttacccg cttcgcgacc
    11161 tctttctgcg ctatctccgg gctcatgctc tggtcacggc tgaacaactg gctcatgagt
    11221 ttagtctcgg tattgccatt gtcgaagagc agcttcagca actgcgcgaa cagggtctgg
    11281 tgatgaatct gcaacaagac atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc
    11341 gctcgctgca agccgccaga gaagcgacgc gtcccgttgc ggccacgacc tatgcgcgat
    11401 tgctgctgga acgtcagggc gtattacccg ccaccgatgg tagcccggcg ctctttgcct
    11461 caacatcgcc aggcgtttat gagggcgtgg atggcgtgat gcgggtgatc gaacagcttg
    11521 ccggagtcgg tttacccgcc tcactctggg aaagccagat cctgcctgcc cgcgtacgcg
    11581 actattcgcc agaaatgctc gatgaattac tggcaaccgg tgcggttatc tggtcggggc
    11641 aaaaaaaact gggtgaagat gacggcctgg tggcactgca tctacaggaa tatgctgcag
    11701 aatcgttcac tcccgctgaa gcggatcagg cgaatcgttc ggcgctgcaa caagcgatag
    11761 tcgctgttct ggctgacgga ggagcctggt ttgcacaaca aatcagccaa cggatacgcg
    11821 acaaaatcgg cgaatcggtt gatccctcgg cactgcaaga ggcgttatgg gcgctggtct
    11881 ggcaaggcgt catcaccagc gatatttggg caccgttacg cgccctcacc cgcagcagtt
    11941 ccaacgcacg cacctcaact cgccgcagtc accgggctcg tcgtgggcgt cctgtctatg
    12001 cacaacccgt ctcgccgcgg gtatcttaca acacaccaaa tctggctgga cgctggtcgt
    12061 tattgcaggt ggagccacta aacgataccg aaaggatgct ggcgctggcg gaaaatatgc
    12121 tcgaccgcta cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt
    12181 ttccatcgat gcaaacgctt tgtcgaagta tggaagattc cgggcgaatt atgcgaggtc
    12241 gttttgtaga aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgcttgc
    12301 gcgacctggc gacacaagcc gcgcaaacgc gccactatac accagtggcg ctctccgcca
    12361 acgatccggc taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg
    12421 taccaacgcg tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc
    12481 tggcgcaagg tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag
    12541 aggttttcca cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa
    12601 cagaagtgaa tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg
    12661 gattttcaag ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg
    12721 ttcgcatccg tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt
    12781 tagcggcagt ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga
    12841 tatcacaacc gccgaccagc tcgccgtcaa cccacagttg cgggaaggtc ggccagttag
    12901 catatttcgg cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac
    12961 gttcgccaca tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt
    13021 tcggtgaacc tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt
    13081 cgatagtggt gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg
    13141 tagcgggtca gtgcagcatc ggaaaataac attttcatca cgctttagct attttatccc
    13201 tttgctctat ttgttgcatt tcaaatattc gtttcttctc tgatgcgcgg caaactggcg
    13261 tatttataac catttttagc tttcactgct gcattttttt gcgctcgcca acgaaacgta
    13321 ttttttaaca ataaaagcta ttaactttct cttcttctat gcattagaat catcaagttt
    13381 tgtaaatcag acgcaggcat gatagacctg ccttaacaga gggacgctca gtggcgcgga
    13441 taaaccgtat ttcgatcacg ctctgtgctt tactttttac caccctgcct ttaacgccta
    13501 tggcccatgc ttcaaagcaa gccagggaga gttctgctac cactcatatc accaaaaaag
    13561 cagataaaaa gaaaagcacg gcaaccacca aaaagaccca gaaagccagc cagaaaacag
    13621 cgaaaaaagc cgccagtaaa agtacgacca aaagcaaaac cgcttcttcc gttaaaaaat
    13681 cttccattac cgcttctaaa aacgccaaaa ctcgcagcaa acacgccgtc aataaaacgg
    13741 cctcagccag ctttaccgaa aagtgtacca aacgtaaggg ctataaatcg cattgtgtga
    13801 aagtcaaaaa tgccgcgtca ggaactcttg ccgacgcgca caaagcgaag gtgcaaaaag
    13861 ctacgaaagt ggcaatgaat aaactgatgc agcaaattgg taagccatat cgttggggtg
    13921 gcagctcacc gcgtaccggt tttgattgca gcggcctggt ttattacgct tataaagatt
    13981 tggtaaaaat tcgtattccg cgtacggcga atgaaatgta tcacctgcgc gatgcagcgc
    14041 caatcgaacg tagtgaactg aaaaacggcg acctggtctt tttccgtact cagggacgcg
    14101 gtacagccga tcatgtcggt gtgtatgtcg gcaacggcaa atttattcag tcaccgcgta
    14161 cgggtcagga aattcaaatc acttctctca gtgaagacta ctggcagcgc cactatgttg
    14221 gcgctcgtcg ggtaatgacc ccaaaaacac ttcgctaaaa ctttaccctg ttgttacggc
    14281 aacagggtaa gttcatcttt tgtctcacct tttaatttgc taccctatcc atacgcacaa
    14341 taaggctatt gtacgtatgc aaattaataa taaaggagag tagcaatgtc attcgaatta
    14401 cctgcactac catatgctaa agatgctctg gcaccgcaca tttctgcgga aactatcgag
    14461 tatcactacg gcaagcatca tcagacttat gtcactaacc tgaacaacct gattaaaggt
    14521 accgcgtttg aaggtaaatc actggaagag attattcgca gctctgaagg tggcgtattc
    14581 aacaacgcag ctcaggtctg gaaccatact ttctactgga actgcctggc accgaacgcc
    14641 ggtggcgaac cgactggaaa agtcgctgaa gctatcgccg catcttttgg cagctttgcc
    14701 gatttcaaag ctcagtttac tgatgcagcg atcaaaaact ttggttctgg ctggacctgg
    14761 ctggtgaaaa acagcgatgg caaactggct atcgtttcaa cctctaacgc gggtactcca
    14821 ctgaccaccg atgcgactcc gctgctgacc gttgatgtct gggaacacgc atattacatc
    14881 gattatcgca atgcacgtcc tggctatctg gagcacttct gggcgctggt gaactgggaa
    14941 ttcgtagcga aaaatctcgc tgcataataa ctgatggcaa atgcagcatt gcctgatgcg
    15001 cttcgcttac catgccaacg cggatgaggc gttaacagcg cattcggcaa gataaatcgc
    15061 acgttgtcag caactgtaac gcagaaggtt atccttctgc gtttttgttt aattagctgt
    15121 tagcaacgca aactgtttca ggttgttttc tggctgacat aaacaccagc aataatgcca
    15181 gtcccgcgac aatcgccccc atcaccggca caaagctgta tcccagccca gcggaaatta
    15241 ccgcaccacc agcggctgct cccagcgcat ttccaagatt aaaggcacca atattgactg
    15301 atgaagacag acctggcgct tcactggcga cacgcatcac gcgcatctgt aacggcggta
    15361 cgaccgcaaa ggttgcagcg ccccacacca ccatgctaat agctgcgccg aactcattgc
    15421 gggccaggaa cgggattgcc agcataatca ccatcagcag caacaaaaag cctttcaacg
    15481 tgccgttaac tgaacgatct gccagtttgc cgccgagata gttaccgata gagaatccga
    15541 caccaatcag caccagcatt gccgtgacga acaccggtgt tgcgtgggta atactttgca
    15601 gtaccggaga gatataggtg tagagagtaa acattgcacc agctcccagt accgtcgtca
    15661 gcaatgcaga cagcacctgc ggacgcatta ataccgccag ctcttttttc acttcaggtc
    15721 gtgcccctgc accaccttta ggtaatgaga agaacagact taccattgaa atcactccca
    15781 gccccgccgt tgccagaaat gacatccgcc agccgatggt ttcacccaac caggtcgccg
    15841 ccggcacgcc accgatattt gccagggtta accccataaa catagtggca actgcgctgg
    15901 cctgtttatg ttttggcacc acgcttgcag ccacgactga acccaaacca aaaaatgctc
    15961 catgattcag gctggtcaaa atgcgtgaaa gcatcagggt catataatcc ggcgcgatgg
    16021 cggaaagtac gttgccgagc gtgaaaattg ccatcaggaa aatcaacgca ctgcggcggg
    16081 cacgatgaga aagtagaagc gtcatcagcg gcgcgccaac cattacgcca actgcatagg
    16141 cactgattaa cattccggca gcgggaatcg agacatccac accgcgcgca atgacgggca
    16201 acaagcccat tggcgagaac tccgttgtcc cgataccaaa cgcgccaatc gccagcgcca
    16261 gcaacggata gttaattttc atgccttatc tccacctctt cgcgtcatta cgcgatattc
    16321 attaaagtgg cgaaagcatg acagcaatca caaaaaaatg aaaataacaa aaagagaaaa
    16381 cacttttgcc attttgctaa caaacaggaa ggagatgcga gggagaacgc gctccctcga
    16441 gaggaaatca gtgcagcgcg gcagtcaaac ccacggctac gatcaaaccg aggacgataa
    16501 tcgttgttac cagtgaaaat ttaaggtcgg tgctcatcaa gttttctcct tttttattac
    16561 cacacaaaaa gtgatattac gcatttttac acactgtgat gaaaaaatct cccgtcattt
    16621 ataatgataa gtgtttttac cacttcccct tttcgtcaag atcggccaaa attccacgct
    16681 tacactattt gcgtactggc cattgacccc ttcctgacgc tccgtgtcgt ttttccggcg
    16741 taccgcaaca cttttgttgt gcgtaaggtg tgtaaaggca aacgtttacc ttgcgatttt
    16801 gcaggagctg aagttagggt ctggagtgaa atggaatggc aacaataaaa gatgtagcga
    16861 aacgagcaaa cgtttccact acaactgtgt cacacgtgat caacaaaaca cgtttcgtcg
    16921 ctgaagaaac gcgcaacgcc gtgtgggcag cgattaaaga attacactac tcccctagcg
    16981 cggtggcgcg tagcctgaag gttaaccaca ccaagtctat cggtttgctg gcgaccagca
    17041 gcgaagcggc ctattttgcc gagatcattg aagcagttga aaaaaattgc ttccagaaag
    17101 gttacaccct gattctgggc aatgcgtgga ataatcttga gaaacagcgg gcttatctgt
    17161 cgatgatggc gcaaaaacgc gtcgatggtc tgctggtaat gtgttctgag tacccagagc
    17221 cgttgctggc gatgctggaa gagtatcgcc atatcccaat ggtggtgatg gactggggtg
    17281 aagcaaaagc tgacttcacc gatgcggtca ttgataacgc gttcgaaggc ggctacatgg
    17341 ccgggcgtta tctgatagaa cgcggccatc gcgaaatcgg cgttatcccc ggcccgctgg
    17401 aacgtaacac cggcgcaggc cgccttgccg gttttatgaa ggcgatggaa gaagcgatga
    17461 tcaaggtgcc ggaaagctgg attgtgcagg gtgattttga acctgaatcc ggttatcgcg
    17521 ccatgcagca aatcctgtcg cagccgcatc gccctactgc cgtcttctgt ggtggcgata
    17581 tcatggcaat gggcgcactt tgtgctgctg atgaaatggg gctgcgcgtc ccgcaggatg
    17641 tttcgctgat cggttatgat aacgtgcgca acgcccgcta ttttacgccg gcgctgacca
    17701 cgatccatca gccaaaagac tcgctgggtg aaacagcgtt caacatgctg ttggatcgta
    17761 tcgtcaacaa acgtgaagaa ccgcagtcca ttgaagtgca tccgcgcttg attgaacgcc
    17821 gctccgtggc tgacggcccg ttccgcgact atcgtcgtta atcacccgtt gcgggagtct
    17881 cttccggctc ccgcagccac tccttattca gcgtctcact atcgccgaga tactcaagca
    17941 accaggttaa cgcaggcgac atatcatttt gctgccatgt cagacaacat gccgaatccg
    18001 gaaaggggtt ttccagttct aatgctaccc acttcccctc attaagccac ggtttggcga
    18061 aatgtgttgg caccatccct atgcataatc ctgccgagat acaggttgcc gatgattccc
    18121 agtcaggcac gacaagtctt ttttggttat ccagcaacca ggtaatacgc ttaggtagcg
    18181 ttcgcgaggt gtcttcgcgc accaacgacg gccagttgcg caacgtatca tcgctgaacg
    18241 ggccatccat caacgccagc gggtgatggc tggcaacaac gcaactccag cttagcatcc
    18301 ccatatcccg gaaggcataa cgaccgccta ccggaatcgc gcgtgttgcg ccaatcgcca
    18361 gttccacgcg cccgtcggaa agcgcatccc agacaccgtt gaacacttcc tgaaagacaa
    18421 gaagttcgac atcatcaaaa tggcgataaa aatcaacgat catctgccgt gtacgttctg
    18481 gcctgacaat attatccact gcaatagcta actgaccacg ccagccgttc gctatctgct
    18541 gacattgctg gcgggtgatc tgcatttttt tgacaacaga gcgaccttct ttgagaaacc
    18601 acgccccggc agcggtcagt tccacatcac ggtgacgtcg ttcaaacagc ggcaccgcca
    18661 gccactcttc cagctgacgt acggtatagc tgaccgcaga aggaacgcga tgcaactcct
    18721 gggctgcggc actaaaacta ccattacgcg ctaccgcatc aacaacttcg agtgaatatt
    18781 ctgaccacat agtctgcctg caaaattttt gaaaccagtc atcaaatatt accgtttcac
    18841 aacactaatt tcactcccta cactttgcgg cggtgtttaa ttgagagatt tagagaatat
    18901 acatgcaacc tgggaaaaga tttttagtct ggctggcggg tttgagcgta ctcggttttc
    18961 tggcaaccga tatgtacctg cctgctttcg ccgccataca ggccgatctg caaacgcctg
    19021 cgtctgctgt tagtgccagc cttagtctgt tccttgccgg ttttgccgca gcccagcttc
    19081 tgtgggggcc gctttccgac cgttatggtc gtaaaccggt attattaatc ggcctgacaa
    19141 tttttgcgtt aggtagtctg gggatgctgt gggtagaaaa cgccgctatg ctgctggtat
    19201 tgcgttttgt acaagctgtg ggtgtctgcg ccgcggcggt tatctggcaa gcgttagtga
    19261 cggattatta tccttcacag aaagttaacc gaatttttgc gaccatcatg ccgctggtgg
    19321 gtctatctcc ggccctggct cctctgttag gaagctggct gctggtccat ttttcctggc
    19381 aggcgatttt cgccaccctt tttgccatta ccgtggtgct gattctgcct attttctggc
    19441 tcaaacccac gacgaaggcc cgtaacaata gtcaggatgg tctgaccttt accgacctgc
    19501 tacgttctaa aacctatcgc ggcaacgtgc tgatatacgc ggcctgttca gccagttttt
    19561 ttgcatggct gaccggttca ccgttcatcc ttagtgaaat gggctacagc ccggcagtta
    19621 ttggtttaag ttatgtcccg caaactatcg cgtttctgat tggtggttat ggctgtcgcg
    19681 ccgcgctgca gaaatggcaa ggcaagcagt tattaccgtg gttgctggtg ctgtttgctg
    19741 tcagcgtcat tgcgacctgg gctgcgggct tcattagcca tgtgtcgctg gtcgaaatcc
    19801 tgatcccatt ctgtgtgatg gcgattgcca atggcgcgat ctaccctatt gttgtcgccc
    19861 aggcgctgcg tcccttccca cacgcaactg gtcgcgccgc agcgttgcag aacactctac
    19921 aactgggtct gtgcttcctc gcaagtctgg tagtttcctg gctgatcagt atcagcacgc
    19981 cattgctcac caccaccagc gtgatgttat caacagtagt gctggtcgcg ctgggttaca
    20041 tgatgcaacg ttgtgaagaa gttggctgcc agaatcatgg caatgccgaa gtcgctcata
    20101 gcgaatcaca ctgatctata tcgatatact tatacttagg ctgctaacaa aattttgttg
    20161 tatgattgaa attagcggcc tatactaatt tcgagttgtt aaagctacga taaatattat
    20221 gtttttacgg ggacaggatc gttccctact cactatggat ggtcatttcg gcaagggttc
    20281 ctcctttccc tctgttctac gtcggattat agactcgcgg ttttttctgc gagatttctc
    20341 acaaagccca aaaagcgtct acgctgtttt aaggttctga tcaccgacca gtgatggaga
    20401 aactatgagt tcatcgtgta tagaagaagt cagtgtaccg gatgacaact ggtaccgtat
    20461 cgccaacgaa ttacttagcc gtgccggtat agccattaac ggttctgccc cggcggatat
    20521 tcgtgtgaaa aaccccgatt tttttaaacg cgttctgcaa gaaggctctt tggggttagg
    20581 cgaaagttat atggatggct ggtgggaatg tgaccgactg gatatgtttt ttagcaaagt
    20641 cttacgcgca ggtctcgaga accaactccc ccatcatttc aaagacacgc tgcgtattgc
    20701 cggcgctcgt ctcttcaatc tgcagagtaa aaaacgtgcc tggatagtcg gcaaagagca
    20761 ttatgatttg ggtaatgact tgttcagccg catgcttgat cccttcatgc aatattcctg
    20821 cgcttactgg aaagatgccg ataatctgga atctgcccag caggcgaagc tcaaaatgat
    20881 ttgtgaaaaa ttgcagttaa aaccagggat gcgcgtactg gatattggct gcggctgggg
    20941 cggactggcg cactacatgg cgtctaatta tgacgtaagc gtggttggcg tcaccatttc
    21001 tgccgaacag caaaaaatgg ctcaggaacg ctgtgaaggc ctggatgtca ccattttgct
    21061 gcaagattat cgtgacctga acgaccagtt tgatcgtatt gtttctgtgg ggatgttcga
    21121 gcacgtcgga ccgaaaaatt acgataccta ttttgcggtg gtggatcgta atttgaaacc
    21181 ggaaggtata ttcctgctcc atactatcgg ttcgaaaaaa accgatctga atgttgatcc
    21241 ctggattaac aaatatattt ttccgaacgg ttgcctgccc tctgtacgcc agattgctca
    21301 atccagcgaa ccccactttg tgatggaaga ctggcataac ttcggtgctg attacgatac
    21361 tacgttgatg gcgtggtatg aacgattcct cgctgcatgg ccagaaattg cggataacta
    21421 cagtgaacgc tttaaacgaa tgtttaccta ttatctgaat gcctgcgcag gtgctttccg
    21481 cgcccgtgat attcagctct ggcaggtggt gttctcacgc ggtgttgaaa acggccttcg
    21541 agtcgctcgc taaaggctat tctatcgccc ccgctccggg ggcgatttca gatcaggctt
    21601 ctgtacctgg ttgattcatg gcattttctc gtgccgccag cacacgttct accgtatcta
    21661 ccactgcctg agtttgtgga tcgatttcaa tgttgacgcg tgcgccaagt tttttcttcc
    21721 caagagtcgt gcgttccagt gtttccggaa ttaaatggac gcaaaaacgc gttggcgtga
    21781 cttcgccgac ggtcaggcta ataccgtcga tgccaataaa tcctttgtac aggatatatt
    21841 tcatcaactg actatcctgg actttaaacc agatctggcg attattttcc gaggttaata
    21901 ttttcgccac ttcagcggtg gtcataatat gacctgacat taagtgtccg ccaatttcat
    21961 cactgaattt cgccgcacgc tcaacgttta cccaatcccc cacttttaaa tcgccaagat
    22021 tggtaatgcg taacgtttct ttcatcaggt caaaactgac atggttgccg ttaatttccg
    22081 tcacggtcag gcagcaaccg ttatgcgcca cggacgcacc ggtttccagg ccgtccagca
    22141 tgtggtcggg taactccacc acatgcgtac gaaaatttgg tttctcgtca atcgacacca
    22201 gttttgcggt gccctgtaca atccccgtaa acatacttac aactcctgaa atcagttaag
    22261 acattctgtt cagcacaata gcaggtggca aacgccctta ccagtgaagg ggtaagaatg
    22321 actatttttt cactggagaa ttaataaatc ctcgctacaa tagactgaat ttcccctgct
    22381 tcttcttttt gctgcccatt caggcggctt tttagtctct catataacta caaataaaag
    22441 gtgttcacgt gcagaagtat atcagtgaag cgcgtctgtt attagcatta gcaatcccgg
    22501 tgattctcgc gcaaatcgcc caaactgcga tgggttttgt cgataccgtg atggcgggcg
    22561 gctacagtgc caccgacatg gcggcggtcg ctatcggtac ttctatctgg cttccggcga
    22621 tcctctttgg tcacggactg ctgctggcat taacgccggt tatcgcgcaa ttaaatggtt
    22681 ccggtcgacg tgagcgcatt gcgcatcagg tgcgacaagg tttctggctg gcaggttttg
    22741 tttccgttct cattatgctg gtgctgtgga atgcaggtta cattatccgc tccatggaaa
    22801 acatcgatcc ggctctggcg gacaaagccg tgggttatct gcgtgcgttg ttgtggggcg
    22861 cgccgggata tctgttcttc caggttgccc gtaaccagtg tgaaggtctg gcaaaaacca
    22921 agccgggtat ggtaatgggc tttatcggcc tgctggtgaa catcccggtg aactatatct
    22981 ttatttatgg tcatttcggt atgcctgagc tcggtggcgt tggttgtggc gtggctactg
    23041 cggcggtgta ttgggtcatg ttccttgcca tggtttctta cattaaacgc gcccgctcca
    23101 tgcgcgatat tcgtaacgaa aaaggcaccg cgaaacccga tcctgcggtt atgaaacgac
    23161 tgattcaact cggtttgccg attgcgctgg cactattctt tgaagtgaca ctgtttgccg
    23221 tcgtggctct gttagtgtct ccgctcggta ttgttgatgt cgcaggacac cagattgccc
    23281 tgaactttag ttcactaatg ttcgtgcttc caatgtcgct ggcggcagcg gtaactatcc
    23341 gcgtaggtta tcgtctgggt cagggctcaa cgctggatgc gcaaaccgct gcgcggaccg
    23401 ggcttatggt gggtgtctgt atggcaaccc tgacggccat attcacggtt tcactgcggg
    23461 agcaaatcgc cctgttgtac aacgacaatc ccgaggttgt aacgctggct gcgcatttga
    23521 tgttgctggc ggcggtatat cagatttctg actcaatcca ggtgattggc agtgggattt
    23581 tgcgtggtta taaagatacg cgttccattt tctatatcac ctttacggct tactgggtgc
    23641 tgggcttgcc aagcggctat attctggcac tgaccgatct ggtcgttgaa cctatggggc
    23701 cagcaggctt ctggataggc tttattattg gcctgacgtc ggcagccatt atgatgatgc
    23761 tgcgtatgcg gttcctgcaa cgtatgccgt cagccatcat tctgcaacga gcgtcccgct
    23821 aataaagaca aggcgcaacc ttcacgggtt gcgcctgtat ttttacgcag gctggagcgt
    23881 tgcgccaatc ccgtcttcgt ctggctgtag tttcagtgca ttacacagag tttgccgact
    23941 ggcatctgtt atctcaacgc gtatatcccc ggtaacttcc cctttcacat tgatgaagtc
    24001 aggtgaaatt tctttcgcac tggtttgcag gcaaatcgct ccctctccgc gcaggcgttt
    24061 cactttcagt tgcacatgcg ttgccgcacc agtgatcagc cacagttttc cgccattcag
    24121 attgatgtta ccaatactga aagtgacact gtcacctttt accgtactgc cgtcgagaat
    24181 caatacaccg ccctgctgta cagtaatcgt tgattgttgc agatctgcgc catagccaat
    24241 aatcattgaa gcgccgtcca gtaatgcaac actcccggtc agttcaaccg gtgcgcgatc
    24301 ttcctctacg acaaaatttt cgtccgcgct ggtgggtgac aacaggatca ctgttccacc
    24361 ttgtattacc gcattttcca gcgtaccggc atgcacttca gcccgaccac ccacattaac
    24421 ggacaggttt tgcgtcttac cggaactgta gagctgaaga ccatctgtcc ccagacgata
    24481 gatggctcca ttctcaataa tagtgtcaga gtcttcacca ccgagattat tcatggtgcc
    24541 accagcaagc aacgtatcac tggctttccc cgccagcata aaaacgccgc cctgattaat
    24601 ctcacaattg ctggcttccc cacctggata caccaacaat tcaccacctt catctaccac
    24661 gaccgctgag gttgtcccat tgaccaacag gccgccttct tgatcgagaa taattttttc
    24721 cgcgcgatgt ccttccagta cacgcaggtt accgccattt tccagcaaca aaccgcaggc
    24781 ataaccttta tcaacgctga attcaccttc gggatggcgg ccattaaccg tagcgagcgt
    24841 agagagacta attgcgcctc cctcggactg aacaatatct gtggcaatgg caccgtcgct
    24901 gaccgatagc gtgccattgt taccgtactg tgtgcatttg gcgatcccac cggttgccac
    24961 tttcaaccag ccagcccccg tgaccacggt attttcggct cttccgccag cttcaacaat
    25021 caactcgcct tcagtaagtt gcgtcgcgcc aatgacctcg ccggtcttaa caatttgcac
    25081 attcccgtcg ctcatcaagt ttttcgcatc agatcccatg atttattcct ttgctgcatc
    25141 tgtgtgcctt tattgctacc taagtgtaaa ggctacggag gatttatcca cgacagattt
    25201 gagatggtgg caaacaactc tgtttaaact ctgatacacg aattattggg ttgtatcaga
    25261 tgtaaatgcg atcctgaata aaaatcgccc ttgcaaatca acaaaatata gccaatttgc
    25321 ttaaaagctc tgcaaacggt cgaaatagcg cagaaaatta cgttttgcct cttgccacct
    25381 tcccactctg ccgctaatat tcgtccccgt tgtcacctac aacgttgcgt tcatagctca
    25441 gttggttaga gcaccacctt gacatggtgg gggtcgttgg ttcgagtcca attgaacgca
    25501 ccatcctgcg tccgtagctc agttggttag agcaccacct tgacatggtg ggggtcggtg
    25561 gttcgagtcc actcggacgc accagatttt cttaatctgg tcttctcctt tttccctctg
    25621 cttcttctct gtatccaata cgttaaaaga tttacactgt cttcgtatgc gttatcagaa
    25681 ggagaatcgc tatggcaact ttgttacaac ttcatttttc tttcaatggc ccatttggcg
    25741 acgcaatggc tgagcagctt aaaccgcttg ctgagtcgat taatcaggaa cctggttttc
    25801 tgtggaaggt atggaccgaa agtgaaaaga accacgaagc cggtgggatc taccttttca
    25861 ctgatgaaaa aagcgccctt gcctatctgg aaaaacacac tgcccgactg aaaaatctcg
    25921 gcgttgagga agttgtcgcc aaagttttcg atgtcaatga gccactgagt caaatcaatc
    25981 aggcaaaact cgcctgacag attttaatca agggcggtta gcgccctttt catccctgtc
    26041 tgaaatttct caaattctaa aaatttcaac caaacttatc tgataacact aaattcgaaa
    26101 gaatgcgtac aggtaagtaa caatgaaaaa aattgctatt gtgggtgccg ggcctacggg
    26161 tatctacacc ttattctcgc ttctacagca acaaactcca ctttctattt ctatcttcga
    26221 gcaggctgat gtggccggtg tcgggatgcc atacagtgat gaggaaaact caaaaatgat
    26281 gctggcaaat attgccagta ttgaaatacc gccgatttat tgtacgtatc tcgaatggct
    26341 acaaaagcaa gaagccagcc atctccagcg ttatggcgtt aaaaaagaaa ccttgcacga
    26401 tcgtcagttt ttaccgcgaa ttctgctggg cgaatatttc cgcgatcaat ttttacgatt
    26461 agtagaccag gcacgaaagc aaaaatttgc agtggctgtt tatgaatcat gccaggttac
    26521 cgatctgcaa attacaaatg ctggcgtcat gctcgctaca aatcaggatt tacccagcga
    26581 gacgtttgat ttagcggtga ttgccacggg tcacgtctgg cctgatgaag aagaagcaat
    26641 ccgaacgtat tttcccagcc cgtggtcagg cctgatggaa gcaaaggtcg atgcgtgtaa
    26701 cgtgggtatt atgggaacat ccttgagcgg actggatgcg gcaatggcag tggcaattca
    26761 gcatggttcg ttcattgaag atgataaaca acacgtcgtt tttcaccgcg ataacgcaag
    26821 tgaaaagcta aatattacgt taatgtcgcg cacgggtatt ttacccgaag ccgatttcta
    26881 ttgccctatt ccctacgagc ccttacacat cgtcaccgat caggcattaa atgctgagat
    26941 tcaaaaaggc gaatatggcc ttttggatcg ggtatttaga ttgatagtag aggaaatcaa
    27001 gtttgctgat ccagactgga gtcaacgcat agccttagag agcctgaatg tcgattcctt
    27061 tgctcaagcc tggtttgccg agcgcaaaca acgcgaccca tttgactggg cagaaaaaaa
    27121 tctccaggaa gtcgaacgca ataaacgaga aaaacatact gttccctggc gttatgtcat
    27181 tctgcgtctg catgaagccg tacaggaaat tgttccacat ctgaatgaac acgaccataa
    27241 acggttcagt aaaggccttg cccgggtttt catcgataat tatgcggcaa tcccttcaga
    27301 gtctattcgt cgcctacttg ccttacgtga agcgggaatc attcatattc tcgctctcgg
    27361 tgaagactac aaaatggaaa ttaacgagtc gcgcaccgtc ctgaaaacgg aagacaacag
    27421 ctactcgttt gacgttttta ttgatgcccg cgggcagcgt ccgcttaaag tgaaagatat
    27481 tcctttccct ggactacgcg aacaattaca gaaaacaggg gatgaaatcc ctgatgttgg
    27541 tgaagattat acgttacagc aacccgaaga tattcgtggg cgcgtagcgt tcggcgcgtt
    27601 gccctggttg atgcacgacc agcctttcgt tcagggactt acggcatgtg cagaaattgg
    27661 tgaggcgatg gctcgggcgg tcgtaaaacc tgcctcccgt gcacgtaggc gtctttcgtt
    27721 tgattaaagg taaagctatc agcactcaac aaactgatgc tgttcattaa gacgatagcg
    27781 gactccggct cgaatattgt tttcaccttc aatggcgacg gcgaaacgaa ctctttcgcc
    27841 gtcatgatat gccagtgcgg cgcttcctcc tggcccgaga ataattgagt cgaccacgcc
    27901 cgtgctcact accgttgaat tttcaccgct ggcaatgatg tgtacattgt cgccgctgtt
    27961 ggcaattctg gcatttgcgc caaagctggc gatttgcacc aggtcaccat tactagcgac
    28021 atgacatcgt tcacccaatg tacaaacgcg tactcgcatt ccggtattcg caatccgact
    28081 gctatctcca gcgctgctta tccgcgaaga attgccggtt acagcaatcc gtgcgcgctc
    28141 accggagctg ccaatgtgac tgttaaagcc gacacttcca atccgcacgc tataacccat
    28201 actggcaatt tgtgcagcat acccagcggt tgcgattctg gcgttatcct cacaacagcc
    28261 gatgcgagca tgatcgtcgc tgcgggcaaa ctgactgcaa aatgctctct ctccgggatc
    28321 tgtggccagt ttttgcatag aggaaacttc ctgatgcgcg aagttttcat ccgccagcca
    28381 ttttttccac gcatactcaa caagactctc cgcccagtcc gtatatcctt cctcaatcag
    28441 tgcatgatga atatcagcat aactaccgcc gcgcgggaaa tggcgcagga accagcgata
    28501 catcaccgct ccgatacgcc attctcgcag gtctgctcgt gtaatgatca ttcgttagtg
    28561 ccggtgatag ccatcgacca tgcttttaaa ggtttcgtgt ggcgtacgcc ccgtggtgca
    28621 aagataaaga tgaccgaaga taaaaaagag gcttataaat gccagagcaa aatgcgcctg
    28681 caataaccag tatcttacgc caggaaacac atctcccacg gcttgcggat agagacacag
    28741 cagccccgtc aatagtaaca acggcagcaa tccatacatg acaccaacat aggcaacctg
    28801 ctgtaaggga ttaaatttag actgggttgt tgccgggaaa ggatgttctt ccccctgcat
    28861 aatgccaaac aaataaaatc gcgtttgttt tgccgcccgc tccagccaac cctgacgacg
    28921 aatgcgatag tggtggccat tacccccaac ggcattgatc agaacaaagc cgagccagca
    28981 tgccagtaac aaaaatccgc aaacttcatg caccgcaacc agacttttaa ccgcagttgc
    29041 gcccaccagc gcaaagtgat ttatcagccc acttgccagc aacaatacaa agagtaacgc
    29101 attcgaccag tgccataatc tgaccgcttt gctgtaaaga taaatcttct caccatgatc
    29161 ggtggctgat ttcttcacgc cacgagcacg aagcaaggcg tgcagcccca gcaccaacca
    29221 catcccaacc agcaatactc cggcaatgat caaccacacc ggccaaaatt cgggagtgaa
    29281 ctgtggcaca tagttcgcta actggctctg aaactgttca gcatgttgcg acgggttcat
    29341 acattttcct ttttaatcaa atgttgaccg aaccggcgat acagatgcgg tttcccggca
    29401 ccaggtagct gatattgata gtatttatta tcctgcaacc acgcctgaat ttctgggcta
    29461 tcttcgcgac caaagatcaa tgcgtgttcc gggcaggcgc tgacgcaaat gggcggaaag
    29521 ccttttgcca gtcgggactc agcgcagaaa tcacatttat ctgcaacttt agtgacaggg
    29581 ttaaggtagc gaacctggta cggacaagcg ccaatgcaat aactgcagcc gatgcactgc
    29641 gatttttcta cccgcacgat cccctgctca tcacgccacg acgcccctgt cgggcatacg
    29701 tcgatgcacg gagcatcttc gcagtgctga catgactggc gaaaaaagtg atactgcgtt
    29761 tcgttgtcgt tatcggtgac tggaatatgt gctatcgata aacggcttcc ctgagcgggg
    29821 acgtggttag ttttacggca tgcgcgggcg caaatattac aaccattgca ccgtgactcg
    29881 tcatgaatca tggcataccg aacttccttt tcttgcctcg tgttcgctaa cagagaagac
    29941 gcagaaccag taaaaaagat tactgttccc atccccagaa caaatttgcg tcgagtgaag
    30001 gacatctgat tatcccctaa tgcagtggct gcaactgtcc accctatcca tgacatctcc
    30061 ctgaaatttc aggggttctg aaactgctct acctcgaacc attcgcataa gccataagcc
    30121 ttgccatagg tcataaatcg ctcgctaaac aagtacaaca ccgccgtcga tgactccacc
    30181 tgccgtatat cggcatattc aggtgcaaca tccatcgcgg cgatcgcggc ttcaatctgc
    30241 gcttcctgga agtaatatgg cgcttgcatc agcatggcga ctttgtaagg acgaggatac
    30301 gtctggcact caaaacgaac ggcgtgtgca atggcgcgac aaatgtcctg ctccacaact
    30361 tgcaaagaca tcgccgcgta gttttcactc atggcctgcg ttgagtaata gtaatcatcc
    30421 tgacttcccg tcatacaagc gatatccgcg tagtctccgt tctgcttaat gctttccagc
    30481 aaaactgaca gttcctcttc tgcaaccgaa tagggtggcg agagaaacac ggcgcgcgcc
    30541 accagctgcc cagccacaga atgctggcga atgaactctg ccaggatttc agccggttct
    30601 ggttccgtta ccgtatcaac ggggtgacgc atcccctgca accattgccg cttcgcctcg
    30661 tcaacttcgc tgactttcgc taacggcaat tcatcctgat ggttcatctt acccattcct
    30721 tacgcaggca gtagattgtg tgctgccaga tcggcggcaa tatcttccag ccccagacgc
    30781 tgcaatgttt cgcgggttgg gcaaccaagc tgtgggtccc agcccatttc tttgtagaac
    30841 atcgtgagcg aagcatgcat atcgtcacga tccattttgt cagtaccttc agtaaagacc
    30901 gggatctgcg gatccttgtc gaatacccag gaacagataa gatcgtgttc attacgcata
    30961 tctttggttt gcatcagttt caccgtgtag gcacgatgca atgtaaagat acgctctgca
    31021 gctaaatcta atttttcctg agtcatctct tcgccagtga tcgctttgaa gaatttggct
    31081 tcaagcgcca gatcgccccg gtaattacgg cttttcagcg gcgaaacggt cattggccag
    31141 acccagttgc acagggtgac ggcgttatgc aaacagaccc gcaacagcga ccacttagca
    31201 tatttaattt ttgcgtcatt gatcggcgtg tagtttttgg tttcatcgta agcatcttca
    31261 gatccaaaaa gttctttcgc cacttcacgt tgcagtttca atggcaagcc ggaaccaata
    31321 aagttgatat gggtatgcgt catgcagtca cggttgaaca tacagttaac aatggaaccg
    31381 acctgcgctg acgcttcatt ggcatggtga accggatagc caaacggcga ccagagttta
    31441 tttttcgcgt agccccagta ctcttcaccc aaattccagc gttctgcgat ggcatatgaa
    31501 ccatcagcca ggtggctcag ctcacccaca cgatgcgcca gacggtagta aaaatcttta
    31561 atgaagttaa cgtcacccgc ttccagttga tcccagcgaa tttccgcata ctcttcagct
    31621 ggcagaacac gcttgaacac acctttgctg taacaataag taaagtcgcg atgcagctgc
    31681 ccgtagttac accacaggcc gtagtcatcg aacaaattca gaccaaccag gttaccaatc
    31741 acacgaccat cgtctttatc ttcaaaatct ttcggcccgt tcgggaagat ggtggtatgg
    31801 acaaagtttg ctacacaagt gttaccacct gtactgggca cgccaaactc ttttacccga
    31861 ggaatattca tttgggtcat acaacggatc gggcaagagt ggcagccgct catttttact
    31921 gtgtatttct ctgccgccgg acctaagtca aaaacggatt tataggtacg aaagccgacc
    31981 gtattctgat tgcctggcgg aatttcaccc gtttcaattg gaccgccttc agccgcgccc
    32041 caaaacagcc ctttacgtgc tgtccagcgt gacttgggat ctgaatactc tgcccacgat
    32101 tgcggcgtac ttggcacgac atggttgtta ttcgcaccga taagttcagt catcatgtaa
    32161 tcattgagac gcttcatctc ctgacgatcg gcaatgttga cccctttcgt cccttcaacc
    32221 gcaatcgctt tcaggttttt cgaacccatt attgcgccag ttcccgcacc accgctgtgg
    32281 ttacggctat tcaacatgcc agagagagga acaaggtttt ccccagcctg accaatagcc
    32341 gccacacagg tttccggact ggtcaatcga caaatttctt ccgtcgttgc gcgcgtccct
    32401 tttccccata agaaatcggc tttttccagg ctaactttgt catctttaat cttcagccat
    32461 accggtgatt tcgccttccc ttcgataata atgacgtcgt atccagcgaa tttcatttgc
    32521 gctgcaaaaa agccccccat atgggcatcg acgactaaat ttcctttggt aaaagtagaa
    32581 agtgaggtga tatttacgcg agaactacag ggggcaccag atccagttaa tgggccggta
    32641 gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc ctggcggtac ttcgtcatac
    32701 ataattttgt agccgaagcc catgccaccg acaaaacttt taaacttact ggaatcttcg
    32761 agggtaatat ttcctgtcgt gagattgact cttaatatat tacctgtcca accgttagcc
    32821 atgatttttt cctttgcaag attcacacag taatatcttt ccactcgata atttttaacg
    32881 cccctgttgg gcaggcgttt gcgcattcac cgcataacac acatttcgag gattttttac
    32941 tttcggtatt tacggtggcc atcatccacg ggcatgccgt agtacaggcg ctacagccaa
    33001 tacaacgttt atgatcgacg gtaatacagc cttctttctg ctgccaggta atcgcaccaa
    33061 tcgggcagac gttcatgcat tgcggctctt tgcattgacg gcaggtgtcc gcggtatagt
    33121 tgagatcgcc atacaggccg ccgccagagc caaccccgtt gtcgccaaag aaataattgc
    33181 gatggatttt gatacgggag aagaatgtcc ctactgagcc atcgttgaag ttggtacaag
    33241 agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg ggtcaccagc acccctttgg
    33301 gtgtatttat caaccctact gtgccgctat caatatcttc ctgtttgcaa cccaaaagtg
    33361 acagtaacgc aggggcaatt gttagaccag taaggccttt tcctgatata cgcaagaatt
    33421 ctaatcgcgt taagccaata tctaatagtg gacgatcaac cgggttcatt tatttcgcat
    33481 cctcttcgca aacacagtgc tgacaattca gcagcctgat attaagcatt caactaatac
    33541 gctgctgact ctatccccaa caccagatat tttccctaac ccgatagtgt atgagaaaag
    33601 atttaacgcc gataaactaa tcctttatgg ttaggagaaa aaataatgtg gttattctgg
    33661 gctcttaaca ttgatcgtta tcaattaaaa ttacaaagca ag
//
LOCUS       DAZGBC010000035        33365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGBC010000035 DAZGBC010000000
VERSION     DAZGBC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.035"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..256)
                     /gene="yjjY"
                     /locus_tag="ACHK1J_001702"
     CDS             complement(116..256)
                     /gene="yjjY"
                     /locus_tag="ACHK1J_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO7910988.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            352..1068
                     /gene="arcA"
                     /locus_tag="ACHK1J_001703"
     CDS             352..1068
                     /gene="arcA"
                     /locus_tag="ACHK1J_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO7910989.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1128..2480)
                     /gene="creD"
                     /locus_tag="ACHK1J_001704"
     CDS             complement(1128..2480)
                     /gene="creD"
                     /locus_tag="ACHK1J_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO7910990.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLGELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTTDDELRIWK
                     "
     gene            complement(2538..3962)
                     /gene="creC"
                     /locus_tag="ACHK1J_001705"
     CDS             complement(2538..3962)
                     /gene="creC"
                     /locus_tag="ACHK1J_001705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO7910991.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDHEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3962..4651)
                     /gene="creB"
                     /locus_tag="ACHK1J_001706"
     CDS             complement(3962..4651)
                     /gene="creB"
                     /locus_tag="ACHK1J_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO7910992.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4664..5137)
                     /gene="creA"
                     /locus_tag="ACHK1J_001707"
     CDS             complement(4664..5137)
                     /gene="creA"
                     /locus_tag="ACHK1J_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO7910993.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5348..6217
                     /gene="robA"
                     /locus_tag="ACHK1J_001708"
     CDS             5348..6217
                     /gene="robA"
                     /locus_tag="ACHK1J_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO7910994.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6214..6861)
                     /gene="gpmB"
                     /locus_tag="ACHK1J_001709"
     CDS             complement(6214..6861)
                     /gene="gpmB"
                     /locus_tag="ACHK1J_001709"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO7910995.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6913..7425
                     /gene="yjjX"
                     /locus_tag="ACHK1J_001710"
     CDS             6913..7425
                     /gene="yjjX"
                     /locus_tag="ACHK1J_001710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO7910996.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7572..7898)
                     /gene="trpR"
                     /locus_tag="ACHK1J_001711"
     CDS             complement(7572..7898)
                     /gene="trpR"
                     /locus_tag="ACHK1J_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO7910997.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7988..9925)
                     /gene="sltY"
                     /locus_tag="ACHK1J_001712"
     CDS             complement(7988..9925)
                     /gene="sltY"
                     /locus_tag="ACHK1J_001712"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO7910998.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10136..11803
                     /gene="ettA"
                     /locus_tag="ACHK1J_001713"
     CDS             10136..11803
                     /gene="ettA"
                     /locus_tag="ACHK1J_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO7910999.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11910..12077)
                     /gene="nadS"
                     /locus_tag="ACHK1J_001714"
     CDS             complement(11910..12077)
                     /gene="nadS"
                     /locus_tag="ACHK1J_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO7911000.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12111..13343)
                     /gene="nadR"
                     /locus_tag="ACHK1J_001715"
     CDS             complement(12111..13343)
                     /gene="nadR"
                     /locus_tag="ACHK1J_001715"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO7911001.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13364..14746)
                     /gene="radA"
                     /locus_tag="ACHK1J_001716"
     CDS             complement(13364..14746)
                     /gene="radA"
                     /locus_tag="ACHK1J_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO7911002.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14795..15763)
                     /gene="serB"
                     /locus_tag="ACHK1J_001717"
     CDS             complement(14795..15763)
                     /gene="serB"
                     /locus_tag="ACHK1J_001717"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO7911003.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15869..16513
                     /locus_tag="ACHK1J_001718"
     CDS             15869..16513
                     /locus_tag="ACHK1J_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO7911004.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16541..17557
                     /gene="lplA"
                     /locus_tag="ACHK1J_001719"
     CDS             16541..17557
                     /gene="lplA"
                     /locus_tag="ACHK1J_001719"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO7911005.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            17589..17852
                     /locus_tag="ACHK1J_001720"
     CDS             17589..17852
                     /locus_tag="ACHK1J_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7911006.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(17936..18655)
                     /gene="deoD"
                     /locus_tag="ACHK1J_001721"
     CDS             complement(17936..18655)
                     /gene="deoD"
                     /locus_tag="ACHK1J_001721"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO7911007.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18712..19935)
                     /gene="deoB"
                     /locus_tag="ACHK1J_001722"
     CDS             complement(18712..19935)
                     /gene="deoB"
                     /locus_tag="ACHK1J_001722"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7911008.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(19987..21309)
                     /gene="deoA"
                     /locus_tag="ACHK1J_001723"
     CDS             complement(19987..21309)
                     /gene="deoA"
                     /locus_tag="ACHK1J_001723"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO7911009.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21436..22215)
                     /gene="deoC"
                     /locus_tag="ACHK1J_001724"
     CDS             complement(21436..22215)
                     /gene="deoC"
                     /locus_tag="ACHK1J_001724"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO7911010.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22473..24023
                     /locus_tag="ACHK1J_001725"
     CDS             22473..24023
                     /locus_tag="ACHK1J_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO7911011.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            23995..24858
                     /locus_tag="ACHK1J_001726"
     CDS             23995..24858
                     /locus_tag="ACHK1J_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO7911012.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVEEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(25154..25936)
                     /locus_tag="ACHK1J_001727"
     CDS             complement(25154..25936)
                     /locus_tag="ACHK1J_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7911013.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEHAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(25933..27006)
                     /locus_tag="ACHK1J_001728"
     CDS             complement(25933..27006)
                     /locus_tag="ACHK1J_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO7911014.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(27128..27289)
                     /locus_tag="ACHK1J_001729"
     CDS             complement(27128..27289)
                     /locus_tag="ACHK1J_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO7911015.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27416..28021)
                     /gene="osmY"
                     /locus_tag="ACHK1J_001730"
     CDS             complement(27416..28021)
                     /gene="osmY"
                     /locus_tag="ACHK1J_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO7911016.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28414..30000)
                     /gene="prfC"
                     /locus_tag="ACHK1J_001731"
     CDS             complement(28414..30000)
                     /gene="prfC"
                     /locus_tag="ACHK1J_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO7911017.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            30220..30468
                     /locus_tag="ACHK1J_001732"
     CDS             30220..30468
                     /locus_tag="ACHK1J_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7911018.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            30895..31008
                     /locus_tag="ACHK1J_001733"
     CDS             30895..31008
                     /locus_tag="ACHK1J_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO7911019.1"
                     /translation="MNSILIITSLLIIFSIFGHALIKLGIGISNNPDKTDV"
     gene            31077..31310
                     /gene="ydfK"
                     /locus_tag="ACHK1J_001734"
     CDS             31077..31310
                     /gene="ydfK"
                     /locus_tag="ACHK1J_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO7911020.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKV"
     gene            31691..32281
                     /locus_tag="ACHK1J_001735"
     CDS             31691..32281
                     /locus_tag="ACHK1J_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7911021.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(32379..32954)
                     /locus_tag="ACHK1J_001736"
     CDS             complement(32379..32954)
                     /locus_tag="ACHK1J_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7911022.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTSQDIEWPALP"
     gene            complement(32954..>33365)
                     /locus_tag="ACHK1J_001737"
     CDS             complement(32954..>33365)
                     /locus_tag="ACHK1J_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911023.1"
                     /translation="SSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSV
                     TGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         8073 a         9054 c         8311 g         7927 t
ORIGIN      
        1 acgaaagcta gcatttagat acgatgattt catcaaactg ttaacgtgct acaattgaac
       61 ttgatatatg tcaacgaagc gtagttttat tgggtgtccg gcccctctta gcctgttatg
      121 ttgctgttaa aatggttagg atgacagccg tttttgacac tgtcgggtcc tgagggaaag
      181 tacccacgac caagctaatg atgttgttga cgttgatgga aagtgcatca agaacgcaat
      241 tacgtacttt agtcatgtta cgccgatcat gttaatttgc agcatgcatc aggcaggtca
      301 gggacttttg tacttcctgt ttcgatttag ttggcaattt aggtagcaaa catgcagacc
      361 ccgcacattc ttatcgttga agacgagttg gtaacacgca acacgttgaa aagtattttc
      421 gaagcggaag gctatgatgt tttcgaagcg acagatggcg cggaaatgca tcagatcctc
      481 tctgaatatg acatcaacct ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt
      541 ctgttagcgc gtgaactgcg cgagcaggcg aatgttgcgt tgatgttcct gactggccgt
      601 gacaacgaag tcgataaaat tctcggcctc gaaatcggtg cagatgacta catcaccaaa
      661 ccgttcaacc cgcgtgaact gacgattcgt gcacgcaacc tgctgtcccg taccatgaat
      721 ctgggtactg tcagcgaaga acgtcgtagc gttgaaagct acaagttcaa tggttgggaa
      781 ctggacatca acagccgttc attgatcggc cctgatggcg agcagtacaa gctgccgcgc
      841 agcgagttcc gcgccatgct tcacttctgt gaaaacccag gcaaaattca gtcccgtgct
      901 gaactgctga agaaaatgac cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg
      961 acgatccgcc gtattcgtaa acatttcgaa tctacgccgg atacgccgga aatcatcgcc
     1021 accattcacg gtgaaggtta tcgcttctgc ggtgatctgg aagattaatc ggctttacca
     1081 ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt ttcaacctta tttccagata
     1141 cgtaattcat cgtccgttgt aacgtcttta ctggctttca ttttcggcag tgaaaacgca
     1201 taccagtcga tattacgagt cacaaacatc atgccggcca gcgccaccac cagcacactg
     1261 gttcccaaca acagcgcgct atcggcagag ttgagcagtc cccacatcac accatccagc
     1321 aacaacagcg cgagggtaaa caacatgctg ttgcgccagc ctttcaatac cgcctgcaaa
     1381 taaataccgt tcattaacgc cccaatcaga ctggcgatta tccatgccac ggtaaaaccg
     1441 gtatgttcag aaagcgccag caagagcaaa taaaacatca ccaatgaaag ccccaccagc
     1501 aaatattgca ttgggtgtaa gcgttgcgcg gtgagcgttt caaaaacaaa gaacgccata
     1561 aaagtcagcg caatcagcag aatggcgtac ttagtcgccc ggtccgttaa ttggtattga
     1621 tcggcaggcg tcgtcactgc gacgctaaac gcggggaagt tttcccagcc ggtatcattg
     1681 cctgaagcaa aacgctcacc gagattatta gcaaaccagc tgctttgcca ttgcgcctga
     1741 aaacctgact cgctaacttc ccgtttggct ggcaaaaaat cacctaaaaa actgggatgc
     1801 ggccagttgc tggttaaggt catttcgcta ttacgcccgg caggcaccac agaaagatcg
     1861 ccggtaccgc ttaaattcag ggccatattc agcttcaggt tctgcttgcg ccagtcccct
     1921 tcgggtaaag ggatatgcac gccctgcccg ccttgctcta acccggtgcc gggttcaatg
     1981 gtcagcgccg ttccgttaac ttcaggcgct ttcaccacac caataccacg cgcatccccg
     2041 acgctaatca caataaatgg cttgccaaag gtgatatttg gcgcgtcgag ctcgccaaga
     2101 cgcgaaacat cgaaatcggc ttttagcgtt aaatcactgt gccagacctg accggtataa
     2161 atccctatct tgcgttcttc cacgttctga ttgccatcaa ccatcaatga ctcaggtaac
     2221 caaaaatgga taaaacttcg tttccgctcc acggttttat cctcttcctg caccgtataa
     2281 agctcggtca caggaatagc gataagcggc ccaacgagtt tttgcggccc actggtactt
     2341 tggcgaatcg catcttccac atcgctacgg taatcggcac gttcgacaat cacctgccga
     2401 atcagcatta tcggaatcaa caacagcaat actgcaccaa acagggtggt cattttccag
     2461 aacagggggg atttcaacat agtcttctcc tttgcaatgg cagcaggata cgaaggctat
     2521 gtgggaagaa tttgaagcta tgtgaagtga cggtgaagtc gaagcgaggc cagcacgccg
     2581 ccttcctgca cgttgcgcag cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg
     2641 aacgccaacc ccagaccgct gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt
     2701 tcaaaaatac gtgacagcgc gtagtcagga ataccactac cggtatccag caccttaagc
     2761 gtgacgtgtt catgatccac ttcggcgctt agcgtgatgc aaccgctctc gggagtaaaa
     2821 tcgatggcgt tatccagtaa attccccagc gcctgctcca gtaacgccgg ttcagcagca
     2881 acgttaacct cagtaggcat aacatgcaaa gtgatgtttt tttctgccaa ctgcacggtg
     2941 cgcgcttcgc tgacgcggcg aaataatgcc gccacatcaa cagcagtcag aactacttcc
     3001 tgacgattct ccagtcttgc ctggcgtagt aacgtttcta ccaatgcctg catacgcgca
     3061 ttttgggtca gaatgttgtc ggtaaaacga gccaccactt ccggtggcgg accttcgcgt
     3121 aaaatttccg ccgcgccacg aatcgccgcc agtgggcttt ttagctcatg ggttaacgca
     3181 taaacatact gctcaatata gtttttccct tccagcttca cgcgcatact ttccagcgcc
     3241 tgcgcaagct tacgcagctc gctactgccg agttcaggaa gaggaacggg cttattgtca
     3301 gtgacggaat cggcatagcg agtgagccgg gcaatggagc ggttgatcca ccaaaccatg
     3361 cctgcgccaa tcaccagtgc aatccccagc aaaatggcgc tggcccataa aattcgccgc
     3421 tcgctgcgct taatgaccgg agccatcgcc gcgttcggtt tgcctacgct caaaacgcca
     3481 ataagccgcg agccgtccat aatcggcgcg gcaacataca tcactgaact ttcgggatcg
     3541 gcaggatttt gcaacgtgct gcgcgcacca tactgaccac gcaacgttag ccagacgtca
     3601 ttccagcgcg aataatcctg cccaacggct ttatttgccg aatcgaacaa tactttgccc
     3661 tgcgcatcgg tcatatagac atgatattca ttgcgcactt tgttaatgcc accgatattg
     3721 gcgcgaaacg ggcgatgttg tagctgatta aacgcctgcg ccagttgccc atgcgttggg
     3781 tccccagaga gcaaatcggg acgcgccagc tccgccagca acgttgcggt gtcgatcagc
     3841 gtcccctccg ttgctcttcg cacgcccggt ttaacttctt tgacaaaaat agccagtacg
     3901 aaccaggccg ccaccgccac cagtaaaaaa tagcccagca acaaccgcat gccgatacgc
     3961 attacaggcc cctcaggcta tatcccatgc cgcgatgagt attaatcggt gaaagatcgg
     4021 ggttgatggc gcgcagcttg gcacgcaacg ttttaatgtg ggtatcgacg gtgcgatcgt
     4081 aggtgtcctg cgcatcttcc cacacgctat ccattagttg ctggcgggac cagacgcggc
     4141 ctggcgactt gagtaacgtc ttcagcaata aaaactcata ccgcgtcagc gttaatggcg
     4201 tgtcaaacca gctgatctgc gccgcgggtt cattcaattc aaaatgacca atacggatga
     4261 cgggagacgg cgacgagaac ttcttcaccc gacgcagtaa ggtgcgcacc ctggcgcaca
     4321 cttcgcgggg tgaaaacggt ttagccacat agtcgtcagc gccaatttcc agcccaagca
     4381 ggcgatcgac ctcttcactt cgggccgtca ggaacagtac aggtaacgcc ggatggagcg
     4441 ccagtaactg gcggcacaat tcaaagccgc tgatatcagg caaaccaaca tcgagaatca
     4501 tgacatcggg aacctgctgg cgagctttat ccagcaccgg caagcctcgc tcaaagacct
     4561 cgacggcaaa accttcctgc tgcaacatgt agaccagcgt gtcggctatc ccttgctcat
     4621 cttccactaa ccagaccgtt tcccgttgca taaatcgcct ctgttattgc cgccacggca
     4681 tgacaggaac cgcgctaatc gcgtttttcg gcgaaccttc tacaactttg tcggagtaag
     4741 ccagataagc gagcgcgttg cgtttggcat cataaaagcg cacgacctgt aacgacttaa
     4801 agaccaggga cgtgcgtttt ttgaatacta cctcgccctg agctttgccg tttttaatac
     4861 gatccgacag ttcaatcggc ccgacttgct gacaagaaat ggccgcatcg gaggtatctt
     4921 ccgccaggcc caatccccct ttaatacccc cggttttcgc ccggctcaca taacaggtga
     4981 catttttcac atcgggatcg tcaaaggctt ccacaacaat tttgtgatcc gggccgatca
     5041 ttttaaatac ggtgtcgacc gaaccaatct cttctgcatg agccaatggc cccagcatta
     5101 tcaggcttaa agacaggatc aaatgcttgt atttcatatt gttaccattt ttatctatta
     5161 cacgagcaat tagttcgtca cggtaaataa catattgatt aagaacaaaa tctcaatact
     5221 tttatttccg tcaagaccta aaacatactc tactaagaaa aaaacactga atgctaaaac
     5281 agcaaaaaat gctattatcc aattacctga tgtcaggtgc tcgttgttga aaggatgagg
     5341 atattttatg gatcaggccg gcattattcg cgacctttta atctggctgg aaggtcatct
     5401 ggatcagccc ctgtcgctcg acaatgtagc ggcgaaagca ggttattcca agtggcactt
     5461 acagagaatg tttaaagatg tcactggcca tgctattggc gcgtatattc gtgctcgtcg
     5521 tttgtcgaaa tcggcggtcg cactacgcct gactgcgcgt ccgattctgg acatcgcgct
     5581 gcaataccgc ttcgactctc aacagacatt tacccgcgca ttcaagaagc agtttgccca
     5641 gactcctgca ctttaccgcc gttctcctga atggagcgcc tttggtattc gcccgccgct
     5701 gcgtctgggt gaattcacca tgccagagca caaatttgtc accctggaag atacgccgct
     5761 gattggtgtt acccagagct actcctgttc gctggagcaa atctctgatt tccgccatga
     5821 aatgcgttat cagttctggc acgattttct cggcaacgcg ccgaccattc cgccagtgct
     5881 ctacggcctg aatgaaacgc gtccgagtca ggataaagac gacgaacaag aggtattcta
     5941 taccaccgcg ttagcccagg atcaggcaga tggctatgta ctgacggggc atccggtgat
     6001 gctgcagggc ggcgaatatg tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga
     6061 gtttatcctg acggtatacg gaacgtgcat gccaatgctc aacctgacgc gccgtaaagg
     6121 tcaggatatt gagcgatact acccggcaga agacgccaaa gcgggagatc gcccaattaa
     6181 tctgcgctgt gaactgctga ttcctatccg ccgttaacgc tgcagctcat ctaatgcagg
     6241 ggcgtccaga tgcgagatgt cccctgcggt ttcaacgacc cagccagacg ccagccacag
     6301 gctttcctga taatccacgc gcgaaataga acagttacgc agacgtaagc ggcgctctgc
     6361 ccatgctggt aatccgagaa tcgtactcac cagacatccc agtgcaatac catggctcac
     6421 cagcagcggt cggcttccct gcggtaagtc ccggcaagat tccagtgccg cattgacgcg
     6481 atcgctgagc tcctgcattg actcgccttc aggaatacgc ccatcaacgg tgccattgac
     6541 cagctgccga cgccagttct cttcttcttc ggtcagagaa tcgatatgtc ttttttccag
     6601 cacacccatg tttaattcac gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg
     6661 ggcgatgatt tccgccgtac gccgggtgcg tcctaaatcg ctactgatga tatgcgtaat
     6721 gccaagctct ttggcacggg ttgccacctg catcgcctgt tgctcacctt tggcggtcag
     6781 cgggctgtca gactggccct gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg
     6841 gactaggtat acctgtaaca tgcttttttt ccgtatactg ctgtaacgtt aattttctga
     6901 gtaatactga ttatgcacca agttgtctgt gcgaccacca atcccgctaa aattcaggcc
     6961 attctgcagg catttcacga gatcttcggc gaaggatcct gccatattgc atccgttgcc
     7021 gtcgagagcg gtgtaccgga acagcccttt ggcagtgagg aaacgcgcgc tggcgcacga
     7081 aatcgggtag caaatgcccg ccgtttactt ccagaggctg atttttgggt ggcgattgaa
     7141 gctggcattg atggcgacag cactttcagt tgggtggtga ttgaaaacgc cagccagcgc
     7201 ggcgaggcgc gttcggctac tttgccgttg cctgcggtga ttctggaaaa agtgcgtgaa
     7261 ggtgaagcgc ttggtcccgt gatgtcgcgt tataccggta ttgatgagat tggtcgtaag
     7321 gaaggggcga tcggcgtgtt taccgccggg aaactcactc gcgccagcgt ctatcaccag
     7381 gcggtgatcc tggcgctgag tccgtttcat aatgccgtgt attaagcgcc tgatgcgacg
     7441 ctgccgcgtc ttatcatgcc taccaaacat attgaaatta cgggtatttg taggacggat
     7501 aaggcgttca cgccgcatcc ggcattcggt gcacgatgcc tgatgcgcca cgtcttatca
     7561 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactggcg cagctcgacg
     7621 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcctgc gccgagttca
     7681 ttttttaact cacgctggct catttcgccg cgcaacagct cttcgacaat acgcacgcga
     7741 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
     7801 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
     7861 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
     7921 gggaagcaaa atgcctcccc cggttgtact cgctaaagag tacgatagca tatcataaac
     7981 gtgtggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
     8041 tgaaatagcg gtagtaagcg tcataagcca gcacgttctt cacataaccg cgcgtctcgg
     8101 agaatggaat actctcgaca aatgccactg cgtcgatacg cccggcgctg ttgccaagcc
     8161 aggttcgcac ccgccctggt ccggcgttat aagctgctga ggagaaaata cgattattgc
     8221 caaattgctg ataaacatat tgcaggtaac tggtgccaat gttgatattc gtttccggat
     8281 ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg
     8341 ccgtgcccgg cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat
     8401 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tccttaccgc
     8461 tggtgtagcg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
     8521 acaacttccc ggcgatcgtt gcctgaacgc taagatccca ccattggttg ttgaaagcat
     8581 accgcgccag ttgagcttgc tctgtttttg acttgctctt caccagattg gcccactcgc
     8641 tacgcgcggt gttatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
     8701 cctgagtcag ggcgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
     8761 ctccgatgcg ttgcgctgca accatcgggt agaaaccacg ctgttgcatg agttgatgca
     8821 aaatctcttt tgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
     8881 gccattcatc tttctctttc gcctccatcg gcaaacgcgc cagccaggta ttcaggccgc
     8941 gacgatcgcc agtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg
     9001 agcgcataat ggcgtcatcg cgccatttcg cctgctcgtc ggtgacatcg ttgcccatca
     9061 aacgccaggc gacgatatcg cgcagctcct gaatctgatc ttcattaagc tgctgcgcct
     9121 gggcaagcga cgggatcatc agccgtgcat tttcagcatc ctgccgcgcc acactggcaa
     9181 acgccaccgc cgccatttga cgggtaaaat cggtcgctcc ggttgtacgc gcgaaggtca
     9241 gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
     9301 caggcatctg ccctgccagc actgttacca ggccagtgtt acccgctttc atcgccagac
     9361 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
     9421 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
     9481 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg
     9541 tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca
     9601 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
     9661 caaagttagt gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt
     9721 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
     9781 tttgtcgatt atcccaggcc tgcttgattt gcgcgtaacg actacgctgc tcatccagtg
     9841 agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
     9901 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
     9961 cgtcatgccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    10021 cactttttta cttttactgc gaaggtgatg gaccaactgg gattaggcgg ggaaaaaggc
    10081 tacacttcgc ctttgaaaac tcattatcac gataaacata gaggcgaagt ccaacgtggc
    10141 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    10201 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    10261 cgcgggtaag tccaccctgc tgcgcattat ggcaggcatt gataaagaca tcgaaggtga
    10321 agcgcgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    10381 ggaacacacc gtgcgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa
    10441 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    10501 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata atctgaacgt
    10561 acagctggag cgtgcggcgg atgcgctacg tctgccggac tgggacgcga aaatcgctaa
    10621 cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga
    10681 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cgtggctgga
    10741 acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt acccacgacc gttacttcct
    10801 cgataacgtt gcgggctgga tcctcgaact tgaccgcggt gaaggtattc cgtgggaagg
    10861 taactactcc tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga
    10921 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    10981 ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata
    11041 ccagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    11101 agtgctggaa gtcagcaacc tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct
    11161 gagcttctcg atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa
    11221 atcgaccttg ttccgtatga tctccggtca ggaacagccg gacagcggca ccatcacttt
    11281 gggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa
    11341 aaccgtttgg gaagaagttt ccggcgggct ggatatcatg aagattggca acaccgagat
    11401 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgcgt
    11461 tggtgaactc tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    11521 cggcaacatg ctgctgctcg acgaaccaac taacgacctg gatatcgaaa ccctgcgcgc
    11581 actggaaaac gccctgctgg agttccctgg ctgcgcgatg gttatctcgc acgaccgttg
    11641 gttcctcgac cgtattgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt
    11701 cttcgaaggt aactttaccg agtacgaaga gtacaagaaa cgcacgctgg gcgcagacgc
    11761 gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    11821 gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt
    11881 aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg
    11941 cgtgaacacc ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggtcacc
    12001 cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    12061 tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcatcacgg ttatctctgc
    12121 tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcttcc
    12181 tcttcaaccc gcacaaattc gatattgttc tcttccagca tctccaccag cagattctgg
    12241 aactctttac gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg
    12301 ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac
    12361 ggatgttccc gcccttcgta ctttttgcag aacgcctgag tggtgacaaa atcggtatcg
    12421 ataaatgcca ctttattggc atatttcacc gcaaaatcaa tgtattgagc gtggcccagc
    12481 gcgattttat cgtagtcaga atactgcaat gcgatctcat caccgccgag gtgtgaaaag
    12541 acataatcgc ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt
    12601 accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac
    12661 ggcttcactt cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca
    12721 ccgctgatac tcataaaggt acgtttcgga tcgaccagca ccgtctcgat ccccagatgt
    12781 tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcagatccgg ctggatccct
    12841 ttttcagcca taaacttttt gataccgttg ctccacacat cccagccgtg cggatacggc
    12901 tccatgccct cttcgttgaa agcatgaatg cgaatatttt tctgatattt aaaagtttgc
    12961 aataaccagc gcagacgatc cggcacggtc ggctgctgcg acatggcact gtcttcgaac
    13021 aacgcgcggt cacgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta
    13081 caggcgcgct ggataaggta gatatgtccg gtatgcagtg ggtagaactt accgaatacg
    13141 acaccgatcg ttttcttctg ccggggaaac tcaagcccca aaaaacggtg caacgcctcc
    13201 agcttttgcg cgctggggct tttgattttg gcattcagta actggcttaa ataccctttg
    13261 gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt
    13321 ttcaggtaat caaatgacga catatctccc tccgtatttc tcattataag tcgtcgaaca
    13381 cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt
    13441 tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaga
    13501 ttcgttcctg accgctgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca
    13561 ccaccagatc ctgcggtagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg
    13621 ctaagtcggc gctggtttcg gttaccttta cgccgccgac cacgttcaca aacacatcct
    13681 gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt
    13741 tttgttccag ccccactgcc acgcggcgcg gattcgccat catcgagtga tcgaccagcg
    13801 cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg
    13861 aggtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc
    13921 cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc
    13981 tgcgcaaggt gcgaaaacgg gagtcggcat cgccatccaa aagcaccgaa cagtcgatac
    14041 agtgttccag cactttcggg ccagccagcg aaccatcttt ggttacgtgc cccaccatga
    14101 caatcgccac accgcgcgtt ttggcgaagc gtgtcaaata agccgccgtt tcacgcacct
    14161 gcgccacgct accaggcgat gactgtacat ccgccatatg catcacctgg atcgagtcaa
    14221 ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgttcg atgctggttt
    14281 ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct
    14341 gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca
    14401 gcgtttgcag tagcagcgtg gatttccccg caccagggtt accgccaatc agaatggcac
    14461 ttcctggcac cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac
    14521 gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac
    14581 cggcatagcc gctgagacgc tcgttacgcg ccaccgtagg cgacgcagca agacgcacct
    14641 cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg
    14701 ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta
    14761 atgctgtgcc gcccgcagga tggcgggcga gcaattactt ctgattcagg ctgcctgaga
    14821 ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca
    14881 cttttggctt ggcatggtag gcaatcccca gccctgccgc tttgatcatc ggcaggtcat
    14941 tggctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc
    15001 gagtcagagt tttcgctttg tactgcgcgt ctacgatgtc gccgatcaca ttgccggtaa
    15061 atttaccgtc catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc
    15121 gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg
    15181 tttccagctt gagcaccagt tgcgttaagc ctggcatcag cggcagattt tcacgcacct
    15241 gttgcagaat attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg
    15301 taaaatcgag ttcgccgcgc atcgcccgtt cggttacttc cgccaccatc tcgcccgttc
    15361 cggccagttt ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca
    15421 tcaccagcaa acccggcgtg cgcaggtgcg ggatttttcc cagcggcgcg acatccagct
    15481 gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct
    15541 gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt
    15601 attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg ctacgacctg
    15661 cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata
    15721 aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat
    15781 cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat
    15841 taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctaca
    15901 tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggggcgtc
    15961 atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac
    16021 cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga
    16081 aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg
    16141 tgtgtatgac gaacaaggcg atcttatcgc ccgttctggc gaaagcgtcg aagtgcgcga
    16201 ccggctggcg ctcgacggca aaaaagcggg cggctatttt aaccagcaga ttgtcgaacc
    16261 cattgccggg aaaaacggac cgctcggcta tctgcgcctg acgctcgaca cccatacgct
    16321 cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct
    16381 ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac gtacccgctg
    16441 gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga
    16501 gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct
    16561 catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca
    16621 aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg
    16681 tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg
    16741 cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac gatctcggca atacctgttt
    16801 tacctttatg gctggcaagc cggagtacga taaaaccatc tccacgtcga ttgtgctcaa
    16861 tgctctgaac gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa
    16921 aaccgccgaa ggcgaccgca aagtctcagg atcggcctat cgcgaaacca aagatcgtgg
    16981 cttccaccac ggcaccttgc tgctcaatgc cgacctcagc cgcctggcaa actatctcaa
    17041 tccggataaa aagaaactgg cggcgaaagg cattacctca gtgcgttccc gcgtgaccaa
    17101 cctcaccgag ctgttgccgg ggatcaccca tgagcaggtt tgcgaggcca taaccaaggc
    17161 ctttttcgcc cattatggcg agcgtgtaga agcggaaatc atctccccag acaaaacgcc
    17221 agacttgcca aacttcgccg aaacctttgc ccgtcagagt agctgggaat ggaacttcgg
    17281 tcaggctccg gcattctcgc atctgctgga tgaacgcttt acctggggcg gcgtggaact
    17341 gcatttcgac gttgaaaaag gccatatcac ccgcgcccag gtgtttaccg acagcctcaa
    17401 ccccgcgccg ctggaagccc tcgccggacg actccaaggc tgcctgtacc gcgcagatat
    17461 gctgcaacag gagtgcgaag cgctgttggt tgacttcccg gaacaggaaa aagagctacg
    17521 ggagttatcg gcatggatgg ctggggcggt aaggtaactc gctgattaaa atatccaagg
    17581 aaggaaatat gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc
    17641 agcgtatttc ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa
    17701 gtaaaatgtt aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta
    17761 gctcaaacat caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat
    17821 gcccttcctg gatgacggct gaacataaat gaatagattc gtgaatagat actaaaaagc
    17881 cggagcagtc ccccactccg gcttaacacg acaatcgcct tgcagcgaaa cacaattact
    17941 ctttatcgcc cagcagaacg gattccagtg cgattttgat catgtcgttg aaggtggtct
    18001 gacgctcagc ggcagtggtc tgctcgtgag tgcggatgtg gtcagacacg gtgcagatgg
    18061 tcagggcttt cgcgccaaat tctgcagcga cgccgtagat accagccgct tccatttcca
    18121 cgccgaggat gccgtatttt tccatcacgt cgaacatttc gccgtccgga gagtagaaca
    18181 ggtcagcgga gaacaggtta cccacgcgag catcaatgcc cagtgcttta gctgcatcta
    18241 ctgcgttacg caccatgtcg aagtcagcga tagctgcgaa gtcatggtct ttaaagcgga
    18301 tacggttaac tttggaatcg gtgcaggcac ccataccaat caccacgtca cgcagtttta
    18361 cgtgcggcag aacagcgcca caggaaccaa cgcggataat tttcttcacg ccgaaatcgg
    18421 tgatcagttc tttggtgtag atggagcagg acgggatacc cataccgtga cccattacgg
    18481 aaattttgcg gcctttgtaa gtaccagtga agcccagcat accgcgtacg ttgttcactt
    18541 cacgggcatc ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg tcgcctggca
    18601 tcaaaactac gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta gccattgttt
    18661 tatccttttt gtgacataac aaaggcggag tcaacgctcc gcccaaatcc atcagaacat
    18721 ggctttgcca tattccatat cagaagtacc aaaatatttt gccagagtct ggccgatatc
    18781 cgcgaaggtt tcacggtgac ccagtgagcc cggttttact ttcgggccgt aaaccagtac
    18841 cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc ggatcacagc cgtggtcagc
    18901 ggtaaggatc aggatgtcgt catcgcgcag cagagacatc agctccggca gacggcggtc
    18961 gaacagttcc agacccgcag cataaccggc gacgtcgcga cggtggcccc aggaagagtc
    19021 gaagtcaacg aagttggtga agacgatggt gttatcaccc gcttctttca tctctttgat
    19081 ggtggcgtca aacagcgcgt ccaggccagt cgctttcact tttttggtga taccgcagtt
    19141 ggcgtagatg tccgcaattt taccgacaga aaccacctgg ccgtgttttt catcaaccag
    19201 tttctgcagc acggtcggtg ctggcggctc aacagccagg tcgtgacggt taccggtacg
    19261 ctggaagtta ccggctttgt cgccgataaa cggacgagcg ataacacgac cgatattgta
    19321 gccgccgttg gtcagctctt cacgggcgat ttcgcacagt tcgtagagtt tatccagacc
    19381 gaaagtttct tcatgacagg caatctggaa cacggagtca gcggaggtat agaaaatcgg
    19441 cttgccggtt ttcatgtgct cttcgcccag ttgatccaga atgaccgtac cggaagagtg
    19501 gcagttaccg aggtaacccg gcagattagc gcgttcgacc agtttatcca gcagctcttg
    19561 cgggaagctg ttttcgtgat cggagaaata tccccactca aacagaaccg ggacaccggc
    19621 gatttcccag tgaccagacg gggtatcttt accggatgac atttcgtgcg cccatgcgta
    19681 cgcgccgata acttcagcgt tgccgtccat tcccgccgga atgaaaccgg tagaaccttc
    19741 gtgtgctttc gccagcccca gacgggtcag atttggcaga ttgagcgggc ctttacgacc
    19801 gttatcagct tcgcctttgg cacaagcttc agcgatatga cccagggtgt cagccccgac
    19861 gtcaccaaag cgttctgcat cttctgtagc gccgatgccg aatgagtcca gcaccataat
    19921 aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa atttaaatga tcagatcagt
    19981 ataccgttat tcgctgatac ggcgatagac agttggtgtg ctttccggtg ctttatcggc
    20041 aagtttaatt gccgctttca ccgctttcgc cgcttcctgc cagctgtttt cgtctttcgc
    20101 gtggataacc gccagcggac gctgaccgtc tacctggtcg cccagacgtg ccatatcagt
    20161 aaagccgacg ctgtaatcga tggtgtcaga tgcctgacgg cgtccgccgc ccattgcaac
    20221 cactgccatc cccagcgcgc gggtatccat ttcactgaca aaaccttcgg tatcagcata
    20281 gactgctttc gtcagcatcg ctgtcggcag atacttcgcg tagttctcaa cgaagtcggt
    20341 cgggcctttt tgtgccgcta ccatacgacc aaagacttct gccgctttac cgttgtccag
    20401 caccgcctgc aatttcgcgc gcgcttcggc gtcatctttc gccagtttgc cggagatcag
    20461 catctccaca cacagcgcca tcgtgacatc aaacagacgc gggttacgat attcacccgt
    20521 caggaactgc accgcttcac gaacttcaac cgcgttacct gcactggagg ccagtacctg
    20581 attcatgtcg gtgagcagcg cggtggtgcg cacgccagcg ccgttagcca cgccaacaat
    20641 cgcttcggca agggcttcag agagttcgta ggtcggcata aacgcgccgc tacccacttt
    20701 cacgtccatc accagcgcgt ccagaccttc cgccagtttc ttcgccagga tcgaggcagt
    20761 gatcagcggg atggagtcca cggttgcggt aatatcgcgg gtcgcgtaga aacgtttatc
    20821 tgccggagcc agtgagctgg tctggccaat aatcgccaca ccgacgtctt taataatttc
    20881 gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg atggattcca gtttgtcgag
    20941 cgtaccgcca gtatgaccga ggccacgacc ggagatcatc ggaatatagc cgccgcaggc
    21001 tgcgaccatc ggccccaaca tcagcgaagt gacatcgccg acgccaccgg tggagtgttt
    21061 atcaacaatc ggtccattca gatgcaggct tttccagtcg agaacggttc ctgaatctcg
    21121 catcgccatg gtcagcgaga cacgctcagg cattgtcata tcgtggaaga aaatggtcat
    21181 cgcgagggcg gcaatctgcc cttcggagat agtgttgtcg cgaataccgt tgataaagaa
    21241 acgaatttct tcatcgctca gcgcatgacc atcacgtttt ttacgaataa tttcttgtgc
    21301 gagaaacaag gtaacctccc gaggaaaaga gtatgaggcg gcttttgccg gatgcgtcta
    21361 cgccttatcc ggcctacggg tagatacgtc tcgtaggcct gataagcgca gcgcatcagg
    21421 cgtaaagcat cttacttagt agctgctggc gctcttaccg tcgccgtggc ccagcgcttt
    21481 cagcaggctt gccagcaggc tggaagcgcc aaagcggtag tgacgcgcat cagcccagtc
    21541 agcaccgaac agttcatctg cgatggcgag atatttctgc gcatcttccg cagtacgcac
    21601 gccgcccgcc ggtttgaaac caacggtttt ttctacgccc atatcacgga tcacttccat
    21661 catgatgcgc gcgctttccg gcgtcgcgtt cacagccact ttaccggtag aggttttgat
    21721 gaagtccgca cccgctttga tggagatttc agacgcttta cggatcagcg cttcgtcttt
    21781 cagttcgccg gtttcgatga tcactttcag cagtacattc gctgccgcgc aagcctcttt
    21841 acaggctttc accaggtcaa aaccaacctg ctcgttaccc gccatcagcg cgcggtacgg
    21901 gaacacaacg tcaacttcat cagcaccgta ggcgattgcc gcacgggttt ctgccagtgc
    21961 gatgtcgatg tcgtcgttac cgtgtgggaa gttggttacc gtagcaatac ggatttccgg
    22021 ggtgccctgc tctttcagtg ttttgcgagc aatcgggata aagcgaggat agatacagat
    22081 agcggcggta ttgccgaccg gagttttggc ctgatgacac agagcaatta ctttctcgtc
    22141 ggtgtcgtcg tcattcaggg tggtcaggtc catcaatttc agtgcacgca ggctgcttgc
    22201 tttcagatca gtcatttcat tctcctggct tgtcgccaat aaaattcacc ttgcgagttt
    22261 gttagtattc taacatctac tccgcaacac acttcgatat acatcacaat taaggaaatc
    22321 tacttacaag tttgcatcac tataatgcga tctggttcaa ataattcact ttcaaatgaa
    22381 tgcgtcagtg gtggcaaacg catcaggatc aaagtgaaca tcacgaaact tcttacaatg
    22441 gcgcatcacc ttcggcataa aaggaaaaga agatgcccac ttctcatgaa aatgcactgc
    22501 aacaacgttg ccagcaaatt gtcaccagcc cagtgcttag cccggagcag aagcgccatt
    22561 ttctggcact ggaagcagaa aacaatctgc cttacccaca gcttcctgcc gaagcccgcc
    22621 gcgcgctgga tgaaggtgta atctgcgata tgtttgaagg tcatgcgccg tacaaaccgc
    22681 gctatgtctt acccgattac gcccgttttc tggcgaacgg ttccgaatgg ctggagctgg
    22741 aaggcgcgaa agatcttgat gacgcactct ctctgctgac cattctttac caccacgtac
    22801 cgtcggtcac atcgatgccg gtctacctgg ggcaactgga tgcgttgttg caaccgtatg
    22861 ttagaattct aacacaagac gagatcgatg ttcgaataaa acgtttctgg cgttacctcg
    22921 acagaaccct gccagacgcc tttatgcacg ccaatatcgg cccgtctgat tcgcccatta
    22981 cccgcgcgat cttacgtgca gacgcagagt tgaagcaggt ttcaccgaac ctgaccttta
    23041 tctacgatcc tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg
    23101 aatgtagcaa accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg
    23161 gctacgggat tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg
    23221 tacgccttaa cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc
    23281 gcactctacc gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc
    23341 tctatcaaca atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc
    23401 ctgaacgttt tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt
    23461 gtgagaaaga agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt
    23521 atcgcatcag cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc
    23581 aaaaacgcgc catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg
    23641 gcgcgcgttt gccgtatggc gatgagccag atccgatcac ccatctgcaa actgtcgccc
    23701 cgcatcatgc ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca
    23761 aacgtaatcc gcaggcgctg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg
    23821 aatttaccgc caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt
    23881 tgtcggattt agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg
    23941 aagaagccgc acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac
    24001 agcagatgcg ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt
    24061 cgtctggctc tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg
    24121 acgatgggac gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg
    24181 cagattgttg acggcaaagt ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc
    24241 ctgaagaggt gtccgcaaca tgccacacca atggcgcaat ccatgagcgt ggaggaggtg
    24301 cttagccatg tgcgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa
    24361 gccacgaccc aactgccgtt tgtggtggcg ctgtttactg ctatcaaaaa cgatccgcaa
    24421 ctgcgccacc tcacctgtct ggtggacagt aacggcatgt tgagcgaaac cggctgggaa
    24481 aaattgctcc cggtgtgcga cggcgcaatg ctcgatctca aagcgtgggg gagcgaatgt
    24541 catcagcaat taaccggacg cgataatcag cagattaagc gcagcatctg tttgctggca
    24601 gagcgcggta agctggcgga actgcgtttg ctggtaatcc ctggccaggt ggattatttg
    24661 caacacatcg aagaactggc ggcgtttatc aagggagtcg gcgatgttcc ggtacgcctg
    24721 aacgcgtttc atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg
    24781 gaagacgttg agccgttggc tgatgcgtta cgggagcgcg gggtgagccg gttaatattt
    24841 ccggcgctgt atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggca
    24901 tgataagacg cgacaagcgc cgactgccgg atgcggcgtg aacgccttat ccggcctacg
    24961 gttcggcaca gacttgtagg catgataaga cgcgacaagc gtcgcatcag gcattgtgcg
    25021 ccaactgccg gatgcggcgt gaacgcctta tccggcctac ggttcggcac aaacctgtag
    25081 gcatgataag acgcgttaag cgtcgcatca ggcattatgc gccgactgcc ggatgctgcc
    25141 tacgaagggc aaactaccca cgaacgttaa acaccgcata cgtgttatta agcaacactt
    25201 cggcaatctc atccgcgggt tccgggcgta actcgcaaag cacggcaaac acacgggcag
    25261 catgctccgg gcggttaggc tgcccctgaa aaccgttgag cggcatatcc ggcgcatcgg
    25321 tttccagcaa caacgatgcc agcggtaatt ttgcgatgac atcgcgggtt ttactggcgc
    25381 gtggataggt gatagtaccg cctacgccga ttttgtagcc cagcagtaca aaccgttcag
    25441 cctgttgctg gctgccagaa aatccgtgga caacaccagc acgcggcaaa tcgtggcgtt
    25501 taagatgcat cgccagtttg tcgtgcgtgc gccgtgaatg caggatcacc ggcagatcgt
    25561 agcgtttcgc cagtttcagt tgttcgtcga gtaaccactg ctgcctctca aattgcggat
    25621 cgtcgccgaa gagatccaga ccgatctccc ccaccgccac caccttcgcc ggacgccttt
    25681 ccagcgcctg ctgtagctga tcaagagaca catcgctatg tttttccaac ataccaggat
    25741 gcaagcccaa tgcggcatac agcggttgat aattttccgc taacgcctgc acgcgggcaa
    25801 aattttccgc ctctgtcgcc ggaacaatga tcttgcctac gcccgcttgt gccgcgcgtt
    25861 gcaggctggc ctcttcatcg ccactaaacg gcgggaaatc aaaatggcag tgggtgtcga
    25921 taaaacggca aatcaagcca gatcctcatt gttaaatgtc gggtcgttcg cctgcggagc
    25981 gtcgatcact tcggcaacca gcgtatcgtt ggcgactggc gcaggcggaa tgacaatcga
    26041 ttccggcgtc accactggca ccagatggcg ggtaagcggc gctttttcag ttaatagctt
    26101 gccaaccgtt gccaggaaat aacgaccgca taaacgccca agtttatagt cttcgcgcag
    26161 cgccggaatc cgactgccaa gcgcgatact atgtaatggc ttcggcggat aaatttcgaa
    26221 tatccgcagc ttgcccggtg gtttctcaat aaattgctga atgtcacgat agctggtttc
    26281 atgatgctgc accagattga ccagcggctg caggctactg tcacccagcc agcgttccat
    26341 gcgtttgaac cactgcggcg tgtagtacat ttgtgacggc acagtgcgga tgacgaccaa
    26401 cgttttagcg ccctgccttg ccgcctcttt aaccggaatc gcatcactga tcccgccatc
    26461 caggtagtta atgccttcca gcgacactcc gctacgataa aagccaggta tcgcactgga
    26521 ggcgcgaatc acatccagcc agttttgttt ggttggtaaa aagtaattcg gcgcgtagtc
    26581 atcctgacga caggcgcaca tataaaacga tttgccgctg tcaaacaacc gcgcggcggt
    26641 atccatttgc aacggcatct ggcttgcggt ggcctccacc agccagtcga gatcgataag
    26701 atttcctcca cggacaaagc gcaatggatc gaaaaattcg cgttttgtgg tatagcgcat
    26761 gatgactttg cgcgcgtaac cgggctgatt gcagataaac gccgagaggt tctgcgcccc
    26821 ggcagatgtg ccgagataaa gatcgaaagg attaaactgc gcgcgcataa actcatccag
    26881 cacgccagcc gtgaaaattc cacgctgccc gccgccttcg cacaccagag caattcgtcc
    26941 gggctggaac ggccttagcg acaacggcgc aatattacca agcgttacag gtattcgctg
    27001 ccccacctct gccttcctgt ttttgttgtg aaagaatgac aaagtaacgc aatttattat
    27061 tcgctaaaac cgcaaaagcc agtccgctgg actggctttg gataaaattt tcttaatggt
    27121 ctgaaatcta gggtcgtttt cggcccatga acaaactcac caggaacaga ataatcccga
    27181 cgacaaagac aatttttgct gcgcctgcag cggtaccggc cagaccacca aatccaagtg
    27241 cggcggcgat taacgcgata accagaaata tgatgcccca acgaaacata agcgtctcct
    27301 ttaccatagt taatgtcacc gcttgatatg agcgtgaaaa tcactcaatg cgatattttt
    27361 agtgtggtgc acatcgcgcc tcccgacaaa gttcgggagg acgaattacg acgaattact
    27421 tagttttcag atcattttta acgcttttca caccatctac cgctttggcg atactttcag
    27481 cacggtcact ttgtgcctga gaatctacgg taccggagag ctgaaccacg ccatcggtgg
    27541 tttcaacttt cacatgacgg gaagggacga tatcgtccgc cagcagtttg gctttgattt
    27601 cactggtggt ggcggtgtca cccgcgtagc ccttcaccga gccttcttta gcgtcgcgaa
    27661 cgtgcagttt gtcgctgaca gaggtcaccc cttcaacgcc tttcgccact ttcactgcct
    27721 cttcggcctg ggcctggctt tcaacgaaac cgctcagggt cacgactttt tgatcggttt
    27781 tgacagagat atcggtgctc ttgatgttgt catgatccac cagggccgcc ttcactttcg
    27841 cggtgatggc gctgtcatcc atgaaattac cgactttatt catagagcta tcgacttttt
    27901 gccctgcgct ttcattggta gtctgtgcgt tgttttccgc gtaggcagag ccggtcgcga
    27961 cggcagaggt caacattaca gccagcagag ttttcgaaat cttcagtctt gtcatagtca
    28021 tcgatttatt cctgtatgtt tgctcgtaat ttgagcctgg caacacgagg ttgcattgct
    28081 gaatagggag agacttcacc ctctacagaa gtcaatggtc gccatcacaa aagcgatgag
    28141 tgatgaataa cgaccattac agcctctgaa tcagttatta atatcggtag aatgacaatc
    28201 gacggcttta gatactgata tctacgcatt gaacggtatt taacgccgtc agaaatgtca
    28261 tcactttgtt aaatatagat cacaattttg aaaccgctcg ggatatcagc gaaaacataa
    28321 gcaaaagtga atgttttaag aacattccgt aagcggctaa taaggaaggg aaattgacag
    28381 ggcgcagcgg ctaccgcgcc ctggaggcaa gaattaatgc tcgcgggtct ggtggaactg
    28441 aacgtccgga taacgttcct gtgccaggcg caggttgacc atgctggtag cgatgtaagc
    28501 gaggttatcg ccgccatcaa gcgccagttg gctttcgttc ttacgcttga actcttcgaa
    28561 tttcttcgcg tctgcacatt ctacccagcg ggcagtggca acgttgactg actcatacac
    28621 tgcttcaacg ttgtattcgc tcttcaggcg cgctaccacc acatcaaact gcagcacacc
    28681 aactgcacca acgatcagat cgttgttgga gattggacgg aacacctgca ccgcgccctc
    28741 ttcggaaagc tgtaccagcc ctttgagcag ctgtttttgc ttcagcggat ctttcaggcg
    28801 gatacgacgg aacagttctg gtgcgaagtt cggaataccg gtgaacttca tcatctcacc
    28861 ctgggtaaag gtgtcgccga tctgaatggt gccgtggttg tgcaggccga ggatatcgcc
    28921 cggatacgct tcttcaacgt gcgaacggtc acccgccata aaggtcagcg cgtcggagat
    28981 caccacatct ttcgcagtgc gcacctggcg cagtttcatg cctttttcat atttaccgga
    29041 caccacacgc ataaacgcca cgcggtcgcg gtgtttcggg tccatgttgg cctgaatttt
    29101 aaatacgaag ccggtaaatt tatcttcgct cgcttctacg gtacgggtat cggtctgacg
    29161 cggcatcggc gcaggcgccc actccaccag gccatccagc atatgatcaa cgccgaagtt
    29221 acccagcgca gtaccgaaga ataccggagt gatttcgccc gcaaggaaca gctctttgtc
    29281 gaactcgtta gacgcgcctt taaccagttc cagttcgtca cgcagctgct gtgccagatc
    29341 ttcaccaacc gcagcatcga gatccgggtt attcagccct ttaacaatgc ggacttcctg
    29401 aatggtgtgc cctttaccgc tctgatagag ataggtttca tctttataaa ggtggtaaac
    29461 gcctttaaac agcttgccgc agccaattgg ccaggtgatc ggtgcgcagc caattttcag
    29521 ctcgttctca acttcatcga gcaattccat cgggtcgcgg atatcacggt caagtttgtt
    29581 cataaaggtg aggatcggcg tgtcgcgcag acgggtaact tccatcagct tacgggtacg
    29641 atcttcaaca ccttttgcgg cgtcgataac catcaggcag cagtccaccg ccgtcagggt
    29701 acgataggta tcttccgaga agtcttcgtg ccccggggtg tcgagcaggt taaccaggca
    29761 atcgtgatac ggaaactgca tcacagacgt agtaatggag atcccacgct gcttttccat
    29821 ctccatccag tccgacttag cgtgctggtt ggaaccacgg ccttttactg taccggcagt
    29881 ctgaatggcc tgtccgaaca gcagcacctt ctcggtgatg gtagtcttac cggcgtccgg
    29941 gtgagaaata attgcaaagg ttcttctttt cgctacctct gcggccaaaa ggcttgtcat
    30001 aattgttgtc tctttgatta tgtacaatca tatgtacaaa gaaaaattca tgccgctgat
    30061 tgtacatttt ggaggtatca gagcatagca gaacatgatc agtaggtgta tttgtagcca
    30121 aagaacattt gtacatgcag caatctttgt tctattgaca ctgtttattc atacagttaa
    30181 aaataacact gtatacaaac acagtgtaga gggattttta tgcgtattga gatctgcata
    30241 gccaaagaga aaatgactaa aatgccaaac ggtgctgtgg atgcgttaaa ggaagaatta
    30301 actcgacgca tcagtaagcg ttatgacgat gtagaggtga tcgtaaaagc caccagcaac
    30361 gatggccttt ctgttacgcg caccgccgat aaagattcag ctaaaacttt tgttcaggaa
    30421 actctgaaag atacctggga gtctgctgac gagtggtttg ttcactaatt aacacgtaaa
    30481 atcggtaacg gctggaaatc attcaatact cgcactatcg gaagttcacc agccaaccgc
    30541 agcacgttct tgcatacgac gtgtctgcgg tttttcaacc attcagatac agcactctca
    30601 tcacattcat tcttccgcat taaagtatat atgaccatat caccttaata tttttcttct
    30661 tcagataaaa attgttatct atgtatactt ttaaaccgaa tccgtgtaga gtctctacct
    30721 aagttagttc gcagctaccc cttcagtttg cgccattaac ctcctgactt ttgccgctat
    30781 ctgtaagcat tcctgtatac ctgaaactac agggttcatc tacgaacttt aagaacaccc
    30841 aggataaaaa ttgtcaacta tattatatat aacaccatac taattcgagg ctatatgaac
    30901 agcatactga taatcacttc actacttatc atattcagca tttttggtca tgctctaata
    30961 aaattaggga ttggcatatc caataacccg gacaaaaccg atgtataagt caacatatcc
    31021 tgaatcagac atacaatatt gcaatgaaaa ccaataatat tttaaggaat atcttcatga
    31081 aatcaaaaga caccctaaag tggttccctg cgcagcttcc tgaagtcaga attatcctgg
    31141 gagatgctgt cgtggaagta gcaaaacagg gaagacctat caataccaga acattgcttg
    31201 attacattga aggaaacata aagaaaaagt catggctgga taacaaagaa ttattacaaa
    31261 cagcgatatc agttcttaaa gacaaccaaa atttaaatgg taaagtgtaa tacccccccc
    31321 tgaaaaataa tttttcaatg cattaaaaaa atatcaacaa ctacgttatt ccgcaacaac
    31381 ctactttttt taccattttt ccactttaac aacattttgc tccacttttc cacgaccaaa
    31441 caacttaaaa tctggttgaa ataacataca acactattat tcgtcttcct cgagtccgcc
    31501 cggagttcga aaaacaaacc gagttaaagc catttttcaa aaaatcgatt tttggttaca
    31561 ccaaaattac gggactgcat ccgaattcgt ttattttcga acgtatacat acaaatatgt
    31621 acaataataa gcgaaataat ttttggatat gcattatgat atgtacacca atttcgtata
    31681 gagtctcact atgtctcgaa tttttgctta ctgtcggata tcaacgctgg atcagaccac
    31741 cgaaaatcaa cgccgggaaa tcgaaagtgc aggttttaaa atcaaacctc agcaaataat
    31801 cgaagaacac attagcggct cagcagcaac cagtgagcgt cctggtttta accggttgct
    31861 tgctcgtctg aaatgtggtg atcaattgat tgtgacaaaa ctggatcgcc ttggttgtaa
    31921 tgcaatggat atcaggaaaa cagtggaaca actgaccgaa acaggtatca gagtgcattg
    31981 cttagcattg gggggcattg acctgaccag tccaacaggg aaaatgatga tgcaagtaat
    32041 ttcagcagtc gctgaatttg aacgagacct tttacttgaa cgcactcatt ccgggatagt
    32101 aagagcccgg ggcgcaggga aacgttttgg tcgaccacct gtgttaaatg aagaacagaa
    32161 acaggtggta ttcgaacgaa ttaagtcagg tgtaagtata agtgccattg cccgggaatt
    32221 caaaacctcg cggcaaacca ttttaagagc caaagcaaaa cttcagacac ctgacatata
    32281 aaaaataatc ttggtgtgag atgctttacg tcttccaagc ccccttcctt gccgtaaatg
    32341 gaaagataca tctaattata gaatttatat gttttaccct acggcagtgc tggccattca
    32401 atatcctgtg aagttgacgt atcaacacgg ttcagcaata cccgatactt tttccatgct
    32461 tccagcaacg agatttcttc ctccgttgca atttccagat ctgcagcatc ctgaagcggc
    32521 gcaatatgct cactggctac ctgcatcagg ctgttttttg tttcttccgc ctctcggatc
    32581 cggaacagtt tttctgcttc tgcatctttc acccaggctg tgccgttcca cttctgaaac
    32641 tccccttccg gcgataacca ggtaacattt tccggtaacg gaccgagttc agaaataaat
    32701 aacgcgtccc ctgacgctac gtcataaacc gttttacccc gatggtcttc aacgagatgc
    32761 cacgatgcct catcactgtt gaaaacagcc acaaagccag caggaatatc tggtggtgca
    32821 atatcggtac tgtttgctgg cagacctgta tgaggcggaa tatatgcgtc accttcacca
    32881 ataaattcat tagttccggc cagcagatta taaattttta tggtccgtgg ttgttcactc
    32941 attctgaatg ccattatgca agcctcacaa tgtagttaaa tgcgatgttt ttgacggtgt
    33001 tttccgcgtt accagcagcg ttaacggtga tagtgtgtcc atgtgagcca atcgcaacag
    33061 agtgcgtatg agcaccaata ccgacagtat gtgcgtgagc acctgcagat gcagcggtgc
    33121 cgctgacact atgagtgtgt gctcctgcag cacttgtatt cacactggcc gcagacacta
    33181 ccttatgtac tgacccattt tgtgaccact ggctgactgc tgatgcgcct gtgacactat
    33241 gagtatggtt tccggcactt gcggctgtac cgctcacact gtgcgtatgc gccccggtgt
    33301 tattcgtgga tttagtgccg taatcaaacg acgatgtggt tttcgtcccc aaatccgtac
    33361 tggat
//
LOCUS       DAZGBC010000036        33270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGBC010000036 DAZGBC010000000
VERSION     DAZGBC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.036"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            239..928
                     /gene="paoA"
                     /locus_tag="ACHK1J_001738"
     CDS             239..928
                     /gene="paoA"
                     /locus_tag="ACHK1J_001738"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO7911024.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            925..1881
                     /gene="paoB"
                     /locus_tag="ACHK1J_001739"
     CDS             925..1881
                     /gene="paoB"
                     /locus_tag="ACHK1J_001739"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO7911025.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPDAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            1878..4076
                     /gene="paoC"
                     /locus_tag="ACHK1J_001740"
     CDS             1878..4076
                     /gene="paoC"
                     /locus_tag="ACHK1J_001740"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO7911026.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASTVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            4086..5042
                     /gene="paoD"
                     /locus_tag="ACHK1J_001741"
     CDS             4086..5042
                     /gene="paoD"
                     /locus_tag="ACHK1J_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO7911027.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCEREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLADVASVRLHQEEDSYLPPSS"
     gene            complement(5220..6347)
                     /locus_tag="ACHK1J_001742"
     CDS             complement(5220..6347)
                     /locus_tag="ACHK1J_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911028.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(6489..7547)
                     /locus_tag="ACHK1J_001743"
     CDS             complement(6489..7547)
                     /locus_tag="ACHK1J_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7911029.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDVAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLRYASEITIPTLIVIGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            7812..8696
                     /locus_tag="ACHK1J_001744"
     CDS             7812..8696
                     /locus_tag="ACHK1J_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7911030.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            8939..8995
                     /locus_tag="ACHK1J_001745"
     CDS             8939..8995
                     /locus_tag="ACHK1J_001745"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911031.1"
                     /translation="MVVFVATVEYAYLSLHFY"
     gene            complement(9399..9677)
                     /locus_tag="ACHK1J_001746"
     CDS             complement(9399..9677)
                     /locus_tag="ACHK1J_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911032.1"
                     /translation="MPMYIKLILSIVRRISVNTICSLIVVVTLSLLSFSSVAKTITAV
                     GSTIDSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(9843..10109)
                     /locus_tag="ACHK1J_001747"
                     /pseudo
     CDS             complement(9843..10109)
                     /locus_tag="ACHK1J_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            10115..10522
                     /locus_tag="ACHK1J_001748"
     CDS             10115..10522
                     /locus_tag="ACHK1J_001748"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7911033.1"
                     /translation="MVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEE
                     NRRAFDLAIEPQITTNDLRLMLRLALAGGGITIATQETFRPYIESGRLVSLLDDFLPQ
                     FPGFYLYFPQRRNIAPKLRALIDHVKEWRQQLA"
     gene            complement(10649..10724)
                     /locus_tag="ACHK1J_001749"
     tRNA            complement(10649..10724)
                     /locus_tag="ACHK1J_001749"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10689..10691),aa:Thr,seq:cgt)
     gene            complement(10839..12092)
                     /gene="proA"
                     /locus_tag="ACHK1J_001750"
     CDS             complement(10839..12092)
                     /gene="proA"
                     /locus_tag="ACHK1J_001750"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO7911034.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLDKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRV"
     gene            complement(12104..13207)
                     /gene="proB"
                     /locus_tag="ACHK1J_001751"
     CDS             complement(12104..13207)
                     /gene="proB"
                     /locus_tag="ACHK1J_001751"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO7911035.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            13495..14550
                     /gene="phoE"
                     /locus_tag="ACHK1J_001752"
     CDS             13495..14550
                     /gene="phoE"
                     /locus_tag="ACHK1J_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO7911036.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(14589..14990)
                     /gene="crl"
                     /locus_tag="ACHK1J_001753"
     CDS             complement(14589..14990)
                     /gene="crl"
                     /locus_tag="ACHK1J_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7911037.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(15048..16292)
                     /gene="frsA"
                     /locus_tag="ACHK1J_001754"
     CDS             complement(15048..16292)
                     /gene="frsA"
                     /locus_tag="ACHK1J_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7911038.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(16384..16842)
                     /gene="gpt"
                     /locus_tag="ACHK1J_001755"
     CDS             complement(16384..16842)
                     /gene="gpt"
                     /locus_tag="ACHK1J_001755"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7911039.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            17103..18560
                     /gene="pepD"
                     /locus_tag="ACHK1J_001756"
     CDS             17103..18560
                     /gene="pepD"
                     /locus_tag="ACHK1J_001756"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7911040.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(18617..18925)
                     /locus_tag="ACHK1J_001757"
                     /pseudo
     CDS             complement(18617..18925)
                     /locus_tag="ACHK1J_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(18945..19153)
                     /locus_tag="ACHK1J_001758"
                     /pseudo
     CDS             complement(18945..19153)
                     /locus_tag="ACHK1J_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(19344..19796)
                     /gene="yafP"
                     /locus_tag="ACHK1J_001759"
     CDS             complement(19344..19796)
                     /gene="yafP"
                     /locus_tag="ACHK1J_001759"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7911041.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(19806..20204)
                     /gene="yafO"
                     /locus_tag="ACHK1J_001760"
     CDS             complement(19806..20204)
                     /gene="yafO"
                     /locus_tag="ACHK1J_001760"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO7911042.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(20207..20500)
                     /gene="yafN"
                     /locus_tag="ACHK1J_001761"
     CDS             complement(20207..20500)
                     /gene="yafN"
                     /locus_tag="ACHK1J_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO7911043.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(20552..21607)
                     /gene="dinB"
                     /locus_tag="ACHK1J_001762"
     CDS             complement(20552..21607)
                     /gene="dinB"
                     /locus_tag="ACHK1J_001762"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO7911044.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(21678..22463)
                     /gene="lafU"
                     /locus_tag="ACHK1J_001763"
     CDS             complement(21678..22463)
                     /gene="lafU"
                     /locus_tag="ACHK1J_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO7911045.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            22435..24147
                     /locus_tag="ACHK1J_001764"
                     /pseudo
     CDS             22435..24147
                     /locus_tag="ACHK1J_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(24340..24837)
                     /gene="rayT"
                     /locus_tag="ACHK1J_001765"
     CDS             complement(24340..24837)
                     /gene="rayT"
                     /locus_tag="ACHK1J_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO7911046.1"
                     /translation="MSEYRRYYLKGGSWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKR
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGNITDFSAG
                     ERIIS"
     gene            complement(25013..25762)
                     /gene="yafL"
                     /locus_tag="ACHK1J_001766"
     CDS             complement(25013..25762)
                     /gene="yafL"
                     /locus_tag="ACHK1J_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7911047.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            25972..26232
                     /gene="dinJ"
                     /locus_tag="ACHK1J_001767"
     CDS             25972..26232
                     /gene="dinJ"
                     /locus_tag="ACHK1J_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO7911048.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDLFDKLGI"
     gene            26235..26513
                     /gene="yafQ"
                     /locus_tag="ACHK1J_001768"
     CDS             26235..26513
                     /gene="yafQ"
                     /locus_tag="ACHK1J_001768"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO7911049.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLRFERTGTHAALFG"
     gene            26669..27409
                     /gene="dpaA"
                     /locus_tag="ACHK1J_001769"
     CDS             26669..27409
                     /gene="dpaA"
                     /locus_tag="ACHK1J_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO7911050.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(27380..28147)
                     /gene="yafJ"
                     /locus_tag="ACHK1J_001770"
     CDS             complement(27380..28147)
                     /gene="yafJ"
                     /locus_tag="ACHK1J_001770"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO7911051.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETSNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(28353..28931)
                     /gene="lpcA"
                     /locus_tag="ACHK1J_001771"
     CDS             complement(28353..28931)
                     /gene="lpcA"
                     /locus_tag="ACHK1J_001771"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO7911052.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            29171..31615
                     /gene="fadE"
                     /locus_tag="ACHK1J_001772"
     CDS             29171..31615
                     /gene="fadE"
                     /locus_tag="ACHK1J_001772"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO7911053.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAHSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYKAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKDLGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPIKLPEKVRKVEAA"
     gene            complement(31658..32104)
                     /gene="ivy"
                     /locus_tag="ACHK1J_001773"
     CDS             complement(31658..32104)
                     /gene="ivy"
                     /locus_tag="ACHK1J_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO7911054.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            32285..33055
                     /gene="yafV"
                     /locus_tag="ACHK1J_001774"
     CDS             32285..33055
                     /gene="yafV"
                     /locus_tag="ACHK1J_001774"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO7911055.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLW"
     gene            complement(33113..33270)
                     /locus_tag="ACHK1J_001775"
                     /pseudo
     CDS             complement(33113..33270)
                     /locus_tag="ACHK1J_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         8039 a         8725 c         8634 g         7872 t
ORIGIN      
        1 cagagctgac aatcacaggt ttccttatcg gtttttgcat cgtacaactc aagcaataaa
       61 ccagctccca tcatcatcaa catcccggcg aacatgaatt tattagccgg gctcatagct
      121 gcattcgcgt tttaagaata atcctcctgc tgtcgccgac tatgcttaac gtttaaaaaa
      181 gcatcagcac tctcgcaacg cactcttatt ttccccttta gaataccgga ggcctggtat
      241 gagcaaccaa ggcgaatacc ccgaagataa tcgggttggg aagcacgagc cgcacgattt
      301 tagtttgacc cgtcgcgatc tgattaaagt gagcgccgca acggcggcga ccgccgtggt
      361 ttatcctcat tctacgcttg cggcaagcgt tccggcagct acacccgcgc cagagataat
      421 gcccctgaca cttaaggtga acggcaaaac ggagcagctt gaggtggata cccgaaccac
      481 gctactggac gctttgcgtg aaaatctgca tttgatcggt accaagaaag gttgcgatca
      541 cggacagtgc ggagcctgta ccgtgctggt caatggtcgc aggcttaatg cctgcctgac
      601 gctggcagtc atgcatcagg gggccgagat caccaccatt gaaggcctgg gttcaccaga
      661 taatcttcac cccatgcagg cggcctttat caagcatgat ggcttccagt gcggctactg
      721 cacctccggg caaatttgct catcagtagc ggtgctaaaa gagattcagg acggcattcc
      781 cagtcacgtc acggtcgatt tggtttcccc tccagaaaga actgccgatg agatccgtga
      841 acgtatgagc ggcaacatct gtcgctgtgg tgcatacgct aacatccttg ccgccattga
      901 agacgctgcg ggggagataa aatcatgaag gcgtttacct atgaacgagt gaacaccccg
      961 gccgaggcgg cacttagcgc tcagcgcgta cccgacgcaa aatttatcgc gggcgggacc
     1021 aatctgctgg acctgatgaa gctggaaatt gaaacgccca cccaccttat cgatgtgaac
     1081 ggcctcgggc tcgataagat tgaagtgacc gacgcgggtg ggctgcgcat cggcgcactg
     1141 gtacggaaca ccgacctggc ggctcacgag cgcgtgcgtc gtgattacgc ggtactctcc
     1201 cgcgccctgc tcgctggcgc gtctggtcag ttacgcaatc aggcaaccac cgcaggtaat
     1261 ctgctccagc gcacgcgctg cccctatttt tacgacacca atcagccctg caataagcgc
     1321 ctgcccggga gcggctgcgc ggcgcttgaa ggctttagcc gtcagcacgc ggtggtaggc
     1381 gtaagcgaag cctgcattgc cacccatccg agcgatatgg cggtcgcaat gcggttgctg
     1441 gatgcggtgg tggaaaccat cacgccggag ggaaagactc gcagtatcac actggctgat
     1501 ttttatcacc caccggggaa aacgccgcac attgaaaccg ccctgcttcc cggtgagctt
     1561 atcgttgcgg tgacgttacc tccgccgctc ggcggaaaac atatttaccg taaggtgcgc
     1621 gatcgcgcct cctacgcctt tgccctggta tcggtcgcgg cgattattca gcctgacggc
     1681 agcgggcgcg tcgcgctggg cggagtagca cataagccct ggcgcattga ggctgcggat
     1741 gctcagctat cccagggggc acaggccgtg tatgacgcgc tgttcgccag cgcccatccc
     1801 accgctgaaa acacctttaa actcctgttg gcgaagcgaa cgcttgcctc cgtactggct
     1861 gaagcgaggg cacaggcatg aaatttgata aacccgcagg ggaaaacccg atcgatcagc
     1921 tgaaggttgt cggtcgtccc catgaccgca tcgacggacc gctgaaaact accggcacgg
     1981 cacgctacgc ctacgaatgg catgaagaat cccccaacgc cgcctatggc tatatcgtcg
     2041 gttccgccat tgccaaagga cgcctcaccg cccttgatac ggacgccgcg caaaaagcgc
     2101 cgggcgtact ggctgtcatt accgccagta acgccggggc actcagtaaa ggcgacaaaa
     2161 acaccgccag gctgttaggc ggccccacta ttgagcacta tcatcaggcc attgcgctgg
     2221 tagtggccga gaccttcgaa caggcgcgag cggcggcctc gctggtgcag gcgcactatc
     2281 gccgtaataa aggagcttac tccctggcgg acgaaaaaca ggccgtcagt cagccgccgg
     2341 aagacacgcc cgacaaaaac gtcggtgact ttgacggggc tttctcctcc gctgcggtga
     2401 agattgatgc tacctacacg accccggatc agagccatat ggcgatggag ccgcatgcct
     2461 cgatggccgt ctgggatgga aataagctta ctctctggac ctcaaatcag atgattgact
     2521 ggtgccgcac cgatctggca aaaacgctga aagtgcccgt ggagaatgtg cgtattatct
     2581 ccccgtatat cggcggaggg tttggcggca agctgttcct gagaagcgat gcgctgctgg
     2641 cagccctcgc cgcccgagcg gtgaaacgtc cggttaaagt gatgctcccc cgccccacta
     2701 ttcccaataa caccacgcac cgccccgcca cccttcagca cttgcgtatc ggtgccgacc
     2761 agagcgggaa aatcaccgct atctcacatg aaagctggtc cggaaacctg cccggcggca
     2821 cgccggaaac ggcggtacag caaagcgaat tactctacgc cggggcaaac cgtcataccg
     2881 gcctgcggct cgccacgctt gatttgccgg aagggaacgc catgcgtgcg cccggcgaag
     2941 cccccggtct gatggcgctc gaaatcgcga tcgacgaact ggcggaaaaa gcgggcatcg
     3001 atcccgtcga gtttcgcatc ctgaatgaca ctcaggttga ccccgccgac ccgacgcgcc
     3061 gcttctctcg ccgtcagctt atcgagtgct tgcgcaccgg agcggataaa tttggctgga
     3121 agcagcgcaa cgccacaccc ggacaggtgc gcgacgggga gtggctggtc ggccacggtg
     3181 ttgcggcggg ctttcgcaat aatctgctgg aaaaatcggg tgctcgggtt cacctcgaac
     3241 aaaacggcac cgttaccgta gaaacggaca tgaccgacat tggcaccggc agctacacca
     3301 ttctggccca gacggcagcg gaaatgcttg gcgtaccgct ggagcaggtt gcggttcacc
     3361 tcggcgattc cagtttcccg gtttctgcgg gttctggtgg acaatggggc gcgaatacct
     3421 ccacctccgg cgtttacgcc gcctgtatga agcttcgcga aatgattgcc tcgacagtcg
     3481 ggtttgatcc tgagcagtcg cagtttgccg acggcaagat taccaacggt acccgaagcg
     3541 ccacgctaca tgaggccacc gcaggcggca gactgacagc ggaagagagc attgaattcg
     3601 gaacactgag caaggagtac cagcagtcga cctttgccgg gcattttgtg gaggtcggcg
     3661 tgcatagcgc gacgggagaa gttcgggtcc ggcgtatgct cgctgtgtgt gctgcaggac
     3721 gcatcctgaa tccgaaaact gcacgcagcc aggtcattgg cgcaatgact atgggcatgg
     3781 gcgcggcact gatggaggag ctggcggtgg atgaccgttt gggctacttc gttaatcacg
     3841 atatggcggg gtatgaggtg ccggtccatg cggatatccc aaaacaggag gtgattttcc
     3901 tggatgatac cgaccccata tcctccccga tgaaggccaa aggtgtcggt gagctgggcc
     3961 tgtgcggcgt gagcgcggct atcgccaacg cggtgtataa cgccaccggt attcgggtac
     4021 gggattatcc catcactctg gataagctgc tcgataagct gccggatgtg gtttaaggag
     4081 gaacaatgtc atacccgctt tttgacaaag acgaacactg gcataagcca gagcaggcgt
     4141 ttctcaccga tgaccaccgg accattctgc gcttcgccgt agaggcgcta atgtccggta
     4201 aaggagcggt gctggtgacg ctggtggaga tacgcggcgg cgcggcgcgc ccgctcgggg
     4261 cgcagatggt ggtgcgcgaa gatggtcgtt actgcggttt tgtctctggc ggctgcgtgg
     4321 aggccgctgc cgcttttgaa gcgctggaga tgatgggctc aggctgcgaa cgcgaaattc
     4381 gctatggcga aggttcgccg tggtttgaca tcgttctgcc ctgcggcggt gggatcacgc
     4441 tgacgctcca taaactacgc tcggcacagc ctctgctcgc cgtgctgaac cgcctggaac
     4501 agagaaaacc ggcggggctg cgctacgatc cgcaagcaca atcgttggtg tgcctgccca
     4561 cgcaaacccg aacgggctgg aatctcaatg gctttgaggt ggggttcagg ccatgcgtca
     4621 ggctgatgat ttacggacgt tctcttgagg cgcaggcaac cgcgagtctt gcagcagcca
     4681 caggctatga cagccatatc ttcgatcttt ttccggcctc agccagcgct cagatcgata
     4741 ccgatacggc ggtcattttg ctgtgccatg atctcaaccg agagctgcca gtgttgcagg
     4801 ccgcgcgaga ggcaaaaccc ttttatctcg gcgcattggg cagctatcga acccacacgt
     4861 tacgtctgca aaagctccac gagctgggat ggtccaggga ggagacagcg caaatccggg
     4921 cacccgtcgg gatatttccc aaagcccggg atgcgcatac tctggcactc tccgtgctgg
     4981 cagacgtcgc ctctgtacgt ctccatcagg aggaggattc atacctgccc ccgtcgtcct
     5041 gatccttgcg gccgggcgtg ggaagcgctt tctcacctcc ggggaaatac cataaattaa
     5101 gagaaaaatc tgcagtagcc gttttagaaa atcacctaaa tcgtttaaaa gagcgcccta
     5161 cccatagtgg gtgctcatcg ccttgtgtaa cttattcagc aacgcgcgcc ctcgccggac
     5221 taaagcgcac cggccttctg gcgtgcggta acaaaggtca gtacgcccgc gcaaaggagc
     5281 agcacactac tcatggcaaa cgtactctgc cagccaaggc ggtcaaacac gatgcctcct
     5341 gccgttgatc caagggcgat tgagagctga atcaccgcga ccatgagacc gcctccggct
     5401 tcggcgttgt caggcaacgt acgcgcaatc catgtccacc atccggttgg cgcggcggtg
     5461 gccagcatgc cccagagtcc taacagcagc gagaccgtcc agacgtgatg cccggtaagc
     5521 atcagaccga tggcaatgac ggccatcagt ccgggtatgg ccatcagcgt ggggtagaat
     5581 tttctgttga gaaatgtcga aacaaccagc gtgccaataa aaccggccac gccgataatc
     5641 agcaaaatca acgacaagcc taaggaatta acccgtgtca ccgactccag aaacgggcgc
     5701 acataggtga atagcgcaaa ctgccccatg aaaaacaggc cacaagctat catgccgacc
     5761 acaaccatgc ggcggctaaa taagcggaat accgcgccac atgacgtgtg gcttttattc
     5821 gcatccatgc tgggcagact aatgcactgc cagataaagg cgacaaccgc catgggcacc
     5881 aggcacaaga aagcgcctcg ccatccgacc gtagccccca gatagcttcc cagcggcgcg
     5941 gccacgaccg tcgccagcgc attaccggcg ttgaaaatcg ccagcgcacg cgtgacctga
     6001 tgctgcggca ccagacgaat cgccgttgcc gctgacatag accagaatcc gccaatggcc
     6061 acaccaatca tcgcacgacc gaccatatac atcagataac tggtagccag cgcaataata
     6121 agccccgata cggccatcag aaccgtcatc cccagcagca ggaacttacg attcatcttt
     6181 ccagctagcg ttgaaagcgt caggctggtc agaacagcca gcgcgccgga gatagcaatc
     6241 ccctgccctg caagcccctc cgtcacgcct aaatcgcggg taatcggcgt gagcaggctg
     6301 acgggcataa attcagaagc aatcagcaca aacacgcaca gggtcatagc aaaaataccg
     6361 ccccagtatg cgtgctgatg atgaggtgat ggtgtttggc ttagtgtcga catagtgaaa
     6421 tttcacaaag atcaggataa aagccacctg ccatagaccg gtggctttgc atcaattcag
     6481 aagaggtttt atttcagttt ggtctggaaa aattgttcga acttcgcgaa aggtattttt
     6541 ccggccacgt tatcgtacag gtccacgtga tttgcccctg gtaccaccac caactctttg
     6601 tctttactgc caaccgcctt gtaagcatct tcagcaaaat agcgtgaatg cgctttctca
     6661 ccgatcacaa tcagcgtcgg gatggtgatt tcactggcgt atctcaacag cggcatattc
     6721 ataaatgaca gcggcatcgt tgccgtccac gccccggtag agttaacgga acgttcgtgg
     6781 aagccgcgcg gcatgcgata gtagtcaaag aactctttca gtaccggatg aggatttgcc
     6841 ggcagcgttt ctggcagaat gcgctcgccc gcactgacct tgccgttgct gtcgacatta
     6901 atatcatgac caccgtgagc aaacgttccg ctctgcgcat ccttccagcg ctgttcattc
     6961 agatattgca gaacagcacg acggtcggcg gtagaataac ggtctttgcc atcccccaca
     7021 ccatgaccca tcgcccggct catgtcatac atcacgctgg tagctaccgc tttaacgcgg
     7081 gtatccatcg ccacgtcgtt cagcgccatg ccgccccagc cacaaatacc gagcagcccg
     7141 atacggttgc ggtctacctc tttttgcagc cctaagaaat ccaccgccgc gctgaaatct
     7201 tcagtgttga tatccggtga ggcgacgttt cgcggatagc cgccgctttc cccggtgtaa
     7261 gaaggatcga acgccagggt aacaaaccct cgttccgcca gcgtctgcgc atacaggccg
     7321 ctggattgct ctttcaccgc gccaaatggc ccactgaccg caatcgccgc cagcttgcga
     7381 tcgccacggt ctttaggcag gtaaagatcg cccactaagg tgatgccata tcggttcggg
     7441 aacgagactt tacggtgatc gactttctgg ctctcggcga atgttttatc ccatttatct
     7501 gtcatggaca caggggcatt aggattggtt gaatcagcat aactcattgt cgtgactcca
     7561 cttaatgatg cgaaaagcag catggtcggc atcgcggttt ttagcgttcg ggtgaaggct
     7621 ttcataaaaa tccccataaa aaacaacaga gagatacagg tttccgacgg acaacgctgc
     7681 ggtgctttac tttgatgggg tcattatagt gagcagaatt agtgctgatt agggggcgat
     7741 taatgctaag attaatacca tattgttata aattaatgcg gagattgccg gcatgaaggg
     7801 gggatgtttc aatggcgaaa agggagaact acaacgagct gtacctgttt atgcaggtgg
     7861 tgcgggaagg cagctttacc gcagcggctc agcggctggg tcttgctcag tcaggagtca
     7921 gccgttctgt tcgcgagctt gaagaaaggc tgggtgtcca gctgctagtg cgcaccacgc
     7981 gtaaactgtc gctgacacaa gcaggcgagc agctctacca gaagacggca tctggatttg
     8041 aaatgttaga tcttgggctt gccacactgg cgcactatcg agagacgccc tccggcacgg
     8101 tgcgtatcaa tgccagccag cacgcgattg ataaatgcct gctgccaaag ctggcggtat
     8161 ttaaacagcg atatcctgat atccggcttg aactgataaa tgagagccgg ttcgtcgata
     8221 tcatcgagga aagattcgat gcaggtgtcc gcctggggcc tgaagtcggc cagggtatgg
     8281 tcgcggtacg catcacgccc gatatggaga tggcaattgt ggggactccg gaacattttc
     8341 gtcgctatgg ttttccgcaa actccggcgg atttaaaggc gcatccctgt atcgcctatc
     8401 agtttgccga cggcagcgta taccagtggg aactcaatca ggatgataaa aaaatcactc
     8461 atcgccctga aggacaatgg gccctatccg acagctatat ggaagcagaa gccgcccggc
     8521 tgggcctcgg attggcctat gttcctgttg aactggtcgc ggatgattta gaacacggta
     8581 agcttatcag ggtgttgcag cgttacagcc tgcgaatgga gggattgttt ctctattacc
     8641 ctcatcgcaa cgtctccccc gccctgcgga tggtcattga tacattgaaa atctgaacgt
     8701 taataacgac cgactcttca ctgtgcaaaa ttgtgtcagg tagtccctct gcgttcaatt
     8761 ccagggtctg ctttgagcga gaaagatcag acttatctgt acaaggggac tatccagcaa
     8821 tggagatcat ccaaaagaaa aaaccacctg gctcccctcg acaaccttaa tccgggcatt
     8881 tagcctcaat caacattttc atatctgtca ggttacttca ttatgaaaac cgatcaatat
     8941 cgttgttttt gttgcaacag tagaatacgc gtatttatcc ctccatttct attaataacg
     9001 gcaataaact gttcatttca ctgatattta aaatatgcat ccttttcaag ctgttcagcg
     9061 gcataagaaa acgtattaaa tatgaattcg acgatttaac catttctgtc tgattggtaa
     9121 tcatctgtga ttaatgctgg tctattgatt acgccagcat taaccgctta gggatgtatt
     9181 cttcctgttg atatacagcc ctcgaaaaag agagaaaata aaactgtgtc caatattact
     9241 ctaccactcc agaccgaagc atcggtagaa ttagcagaac gactggtaca atagattggg
     9301 atagcgtcat ttgtcaggta ttccattgcc tgacaaatga caattttgag tcaatagacg
     9361 tgcttcttga tcatgtctta aaaattatac catgagcctt atgaagcatc atttttactg
     9421 actggtgtta attttgctat catataaact ctgttcttaa aaaacgcgcc cagaatttta
     9481 tatgatttcc cttgtttttc tgcctgtaaa gaaatttctt tttcagtgct gtcaatggtt
     9541 gaaccgacgg cagtaatcgt tttggcgacg ctactgaatg ataataaaga cagtgtaaca
     9601 acaacaatta aagaacagat agtattcacg ctaattctcc tgacaattga gaggatgagt
     9661 tttatataca taggtattaa acatgagtca tatttacccc aagagaaaat actccttctg
     9721 taattaaacc ttcatctcct tcggggtgag caatacataa tgcatcttgc tatgaaaaac
     9781 acagggatat ataaatcttc cttctgacct atttaataaa acatgaatgt aaaagttatt
     9841 cttcagtttg cagatgcagt gggtctgatg gtaatttcgg ttgtatcaac gctttcggga
     9901 gattcaataa tatgccttac cgcacgggca atatccgcag gttgaagtgc aacagaacgg
     9961 taggcgtcca ttgccttcat ggtttccaca tgggtaatgg ttgaagccag ttcactttcg
    10021 acaacgccag gatttacgca ggtcacacgg attttcgagc tttcctgccg taagccatca
    10081 gaaatagccc gtactgcaaa tcaggcagcg tgaaatggtc gttgcctctc cctcctatct
    10141 cgcagccaac agcacacctg ttcatccgcg tgaacttgtg aatcataagt gtatcggctg
    10201 gcgtcaatcc ccggatgttg cgccttatcg ctggccattt gaagaaaaca ggagagcttt
    10261 cgaccttgcg attgaaccgc agattacgac taatgatctg cgtctgatgt tgaggctggc
    10321 tcttgccggc ggaggaataa caattgccac tcaggaaact ttcaggccat atattgaaag
    10381 cggtaggctt gtatctctgc ttgatgactt tcttccacaa tttccgggct tctatctgta
    10441 tttcccacag cgtcgcaata ttgcaccaaa gctccgcgcc ctgattgacc acgtcaagga
    10501 atggcggcag caattggctt aaatgtctgc acctgcattg cctgatgtca gaacagtatt
    10561 ttgattaatt gccagggtta caatggcata aatacggcac aggaggaaac gtggtgtatt
    10621 tgaatatggg gtaactactt gatttaaatg gtgccgataa taggagtcga acctacgacc
    10681 ttcgcattac gaatgcgctg ctctaccaac tgagctatat cggccctgaa aggacatgtt
    10741 cacgaacgtg aatcacggtg gacaaggtta aaactaaccg ggcgatgcgt caatggcctt
    10801 gtgaatcaaa tggctacttt tgcatcaccc ggttttattt acacacgaat ggtgtaatca
    10861 ccaatgccga tccacttgta agtggtcagt gcttccagcc ccattgggcc acgcgcgtgg
    10921 agtttttgtg tgcttaccgc cacttccgca cccagtccaa actggccgcc gtcggtaaaa
    10981 cgcgtagagg cgttaacgta aacagcggac gaatccactt cgttaacaaa acgctgggcg
    11041 ttgcgcatat cgcgggtcag gatcgcatcg gagtgttgtg tgccgtgttc acgaatatgg
    11101 gcgatggcat cgtcaagatc gctgacgatt ttgacgttca aatctaatga cagaaactca
    11161 tcgtcatact cttcggcttt aaccgccacc accttcgcgg ggcctgcctg caactgtgtc
    11221 agcgcagctg catctgcgtg taatgtcacg ccgctttccg ccatttgttt gcttaatgcg
    11281 ggcaggaagc tatcggcgat gtttttattc accagcaacg tttctaccgt attacatgtg
    11341 ctcggacgct gagttttcgc gttgacgatc acttttaatg cttcagcgat ctctgcactt
    11401 tcatcaacgt aaatatggca tacgcctata ccacctgtga tcaccgggat cgtcgactgc
    11461 tcgcggcaca gtttatgcaa accagccccg ccgcgcggga tcagcatgtc gatgtattta
    11521 tccatacgca gcatttcact gaccagcgca cggtcaggat tatcaatcgc ctgcacggca
    11581 ccagccggta agctacagga tttcagggcg tcctgaatca ccgccaccgt tgcagcgtta
    11641 gtgcgacagg tttctttgcc accgcgcagg atcaccgcgt tacctgtttt caggcacagc
    11701 gaagcgacat caaccgtcac gttcgggcgc gcttcataaa tcacgccaat aacccccagc
    11761 ggtacgcgac gacgctcaag acgcaggccg ctgtccagta cgccgccatc gattacctgc
    11821 cccaccggat cggcgaggtt gcacacctgg cgcacatcat cggcaatgcc tttcagccgt
    11881 gcgggcgtca gtgccagacg gtcaagcatc gcttcgctga ggccattggc acgcgcgtca
    11941 gcaacatcct gggcgttagc gttgaggatg atttcgcttt gtgcttccag ttcatcggcg
    12001 attttatcca gcacgcgatt tttttcgcgg ctggagagtt gcgctaattt atacgaggct
    12061 tgcttcgcgg caatgcccat ttgttccagc atcagcctgc tccttaacgg gtaatcatgt
    12121 catcacggtg aacggcaacc gggccgtatt catatcccag tattgcatca atttcttgcg
    12181 agtggtgtcc ggcaatacgg cgtaatgcat cgctgttgta acgactgacg ccgtgggcga
    12241 tatcgcgacc ttcgaggtta caaatgcgga tgacttcacc acgcgagaag ttgccagtca
    12301 cgcttttaat gcctttcggc aacagggagc tgccgcgttc aagaatggcg gcggttgccc
    12361 cttcatctac cgtgatttca cccgcaggcg gcgcaccgaa aatccagcgt ttacggtttt
    12421 caagcggagt cgcctgggca tggaacagcg taccgacgga aatgccttcc atcacatcac
    12481 caatgacgcc cggcttgctg cccgcggcaa taatggtgtc gatacccgca cggcaagcca
    12541 cgtccgcggc ctgcaatttg gtactcatgc cgccagttcc gaggcctgaa acgctgtcac
    12601 cggcaatcgc gcgcagtgcg tcatcaatac cgtaaacatc ttttatcaat tctgcctgcg
    12661 gattactgcg cgggtcagcg gtatacaaac ctttttgatc ggtcagtaac aacagtttat
    12721 cggcacccgc cagaatcgcc gccagtgcag aaaggttgtc gttatcgccg actttaatct
    12781 ctgccgtagc gacagcatcg ttctcattga ttaccggaac gatattgtta tcgagcaacg
    12841 cacgcagggt gtcgcgggcg ttcaggaagc gttcacggtc ttccatatca gcacgagtca
    12901 gcagcatttg cccgacgtga atgccataaa tcgaaaacag ctgttcccac agttgaatca
    12961 gtcgactctg ccctaccgcc gccagcagtt gtttcgaggc gatagtcgct ggcagttccg
    13021 ggtaacccag gtgctcacgt ccggcggcga tcgcgcccga cgtcacaata acaatccgat
    13081 gcccggcggc atgtaactgc gcgcactggc gaacaagttc aacgatatgg gcacggttca
    13141 gacggcgcga tccgcctgtt agcacactgg tgccgagttt taccaccagc gtctggctgt
    13201 cactcatgat tctctgccat tcaattttag gaaaaatgat atcaaacgaa cgttttagca
    13261 ggactgtcgt cggttgccaa ccatctgcga gcaaagcatg gtgttttgtt gcgcgggatc
    13321 agcaagcgta gcggcagttg tttacgcttt tattacagat ttactaaatt accacatttt
    13381 aagaatatca ttaatctgta atatatcttt aacaatctca ggttaaaaac tttcctgttt
    13441 tcaacggggc tctcccgcag aatattcgcg cgttaattaa aatcaggaat gaaaatgaaa
    13501 aagagcactc tggcattagt ggtgatgggc attgtggcat ctgcatccgt acaggccgca
    13561 gaaatatata acaaagacgg taataaactg gatgtctatg gcaaagttaa agccatgcat
    13621 tatatgagtg ataacgacag taaagatggc gaccagagtt atatccgttt tggttttaaa
    13681 ggcgaaacac aaattaacga tcaactgact ggttatggtc gttgggaagc agagtttgcc
    13741 ggtaataaag cagagagtga tactgcacag caaaaaacgc gtctcgcttt tgccggattg
    13801 aaatataaag atttgggttc tttcgattat ggtcgtaacc tgggcgcgtt gtatgacgtg
    13861 gaagcctgga ccgatatgtt cccggaattt ggtggcgact cctcggcgca gaccgacaac
    13921 tttatgacca aacgcgccag cggtctggcg acgtatcgga acaccgactt cttcggcgtt
    13981 atcgatggcc tgaacttaac cctgcaatat caagggaaaa acgaaaaccg cgacgttaaa
    14041 aagcaaaacg gcgatggctt cggcacgtca ttgacatatg actttggcgg cagcgatttc
    14101 gccattagtg gggcctatac caactcagat cgcaccaacg agcagaacct gcaaagccgt
    14161 ggcacaggca agcgtgcaga agcgtgggca acaggtctga aatacgatgc caataatatt
    14221 tatctggcaa ctttttattc tgaaacacgc aaaatgacgc caataactgg cggctttgcc
    14281 aataagacac agaactttga agcggtcgct caataccagt ttgattttgg tctgcgtcca
    14341 tcgctgggtt atgtcttatc gaaagggaaa gatattgaag gtatcggtga tgaagatctg
    14401 gtcaattata tcgatgtcgg ggctacatat tatttcaaca aaaatatgtc agcgtttgtt
    14461 gattataaaa tcaaccaact ggatagcgat aacaaattga atattaataa tgatgatatt
    14521 gtcgcggttg gcatgaccta tcagttttaa tgattattgc cggatgtgat gcatccggca
    14581 catttcactc acgccgttaa cttcaccggc tcgtcacgaa aatcatccgc cggttccagc
    14641 ttcagattca gcgtcgtcag cagttcacgc agtttctcgt gaaactcacg cagggtgtgc
    14701 tccagtcgtt caaccacttc agtgtctttt accggaacgc tcttccagtc gcctgcttta
    14761 tcgaacagac caaactggta actgtaggta aaacgggatt cctgcgcttc aagctccatc
    14821 caccagcccc agaactcacg cacttccggt gccggtttca cgttgacgca tacagccaga
    14881 caatcgaaaa agaatcgatt atcttcgcac ttaccttcac gaatatacgg gcctagtgcg
    14941 gtaaattttt tgatcaatct gctcttcggg tgtccactcg gtaacgtcat tgctatctcc
    15001 tgttgtgatg caactgtttt accaaattgg caaaatttag caaattttta acacaagcgt
    15061 ttttcgatcc agtcggtgat ttcctgaaga cctttgtcaa aattccgata caccgggtta
    15121 aatgggatct ctaataattt accgtcagca gatgatgagg tgattaagcg tgagtcctct
    15181 tccgggctga acggatcgtt cttccagtag cctgataaca ttggcgttgg gcagcgacgt
    15241 cccagcaatc cttgcacttt taatgaatag cgattcagct ccacgcgcaa cgcttcatcg
    15301 gaagcatcat gcatccccaa acgactcgcc agaacgtcaa gatacatttc cggcacctgt
    15361 tgctggcact taaaatcact caacagggta tgaactaccg gaccaagaca ggcaaccgct
    15421 ttcagacgcg gcgattcaag atatgccaga cgcacggcaa cgttagcgcc gaaacggaaa
    15481 ccaaaggccg cgacgcgagt gtgatccacc cacggtacgt taggcagcgc ctttaagacg
    15541 tgctgatgca acaggctgga gtcctgggtg agcttccatt ttgatgaaaa gcccaccgac
    15601 ggcatatcaa tagtcagcat cgcaatgccg cgcggcgcaa aataacgttc atacaggctg
    15661 taatagtccg tctgcatcgc atccagacca ccacacatta atactgtcgg gaacgggcca
    15721 tcgcctttcg gcatatgcaa aaagccggtg atgggcgcac cgccgggtac ggtaaactcc
    15781 atctgccgca tcgtgcccgg cagacgctga gcggcctctt cataggcgcg gtttgacaaa
    15841 gcctgcgctt gctcggccag gtcatctcct ttcagatgag gataggcggc aatgttgtac
    15901 aacgtagccg catgcagcca gtgacgacca ctgagttgcg gatcttcttc cgcacaggct
    15961 ttttgttgcc acaccatcgc ctgggtggcc cactcataaa tccagttgcc gccacggtag
    16021 ccaatcaccg tatcgtataa atcatcgtcg gtacgttcgg catcgctcat cacaatacgt
    16081 gcctggacgt cgaggatttc gcgtgggtca atgccgcgcc agatccacat cagacggtta
    16141 atcatgcgat accagtgagg gatggtttta ccatcaaggg ccgactgcac aggcggttgt
    16201 gcgccgtgat taaagcggcg gactagcgtc gaggtttcag gatgtttaaa gcggggtttg
    16261 aacagggttt cgctcaggtt tgcctgtgtc atggatgcag cctccagaat acttactgga
    16321 aactattgta acccgcctga agttaaaaag aacaacgccc ggcagtgcca ggcgttgaaa
    16381 agattagcga ccggagattg gcgggacgaa tacgacgccc atatcccacg gctgttcgat
    16441 ccaggtatct tgcgggatat caacaacata atcatcaacc agcggacgac cagccggttt
    16501 tgcaaagatg gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa ccgcagtacc
    16561 accggtatcc accaggtcat caataacgat gaagccttcg ccatcgcctt ctgcgcgttt
    16621 cagcacttta agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa cggtatcgac
    16681 atgacgaata cccagttcac gcgccagtaa cgcacccggt accagaccgc cacggcttac
    16741 ggcaataatg cctttccatt gttcagaagg catcagtcgg cttgcgagtt tacgtgcatg
    16801 gatctgcaac atgtcccagg tgacgatgta tttttcgctc atgtgaagtg tcccagcctg
    16861 tttatctacg gcttaaaaag tgttcgaggg gaaaataggt tgcgcgagat tatagagatc
    16921 tggcgcacta aaaaccagta tttcacatga gtccgcgtct ttttacgcac tgcctctccc
    16981 tgacgcggga taaagtggta ttctcaaaca tatctcgcaa gcctgtcttg tgttgacaac
    17041 attttctgct aaccctgtga cctgcaatac tgttttgcgg gtgatcgaca aggagactta
    17101 acgtgtctga actgtctcaa ttatctccac agccgctgtg ggatattttt gccaaaatct
    17161 gttctattcc tcacccgtcc tatcatgaag agcaactcgc tgaatacatt gttggttggg
    17221 caaaagagaa aggtttccat gtcgaacgcg atcaggtagg taatatcttg attcgtaaac
    17281 ctgctacagc aggtatggaa aatcgtaaac cggtcgtctt gcaggcccac ctcgatatgg
    17341 tgccgcagaa aaataacgac accgtgcatg acttcacgaa agatcctatc cagccttata
    17401 ttgatggcga atgggttaaa gcgcgcggca ccacgctggg tgcggataac ggcattggta
    17461 tggcctctgc gctggcggtt ctggctgacg aaaacgtggt tcacggcccg ctggaagtgc
    17521 tgctgaccat gaccgaagaa gccggtatgg acggtgcgtt cggcttacag agcaactggt
    17581 tgcaggctga tattctgatt aacaccgact ccgaagaaga aggtgaaatc tacatgggtt
    17641 gcgcgggggg tatcgacttc acctccaacc tgcatttaga tcgtgaagag gttccagctg
    17701 gttttgaaac cttcaagtta accttaaaag gtctgaaagg cggtcactcc ggcggtgaaa
    17761 tccacgttgg cctcggtaat gccaacaaac tgctggtgcg cttcctggcg ggtcatgcag
    17821 aagaactgga tctgcgtctt attgatttca acggcggcac actgcgtaac gccatcccgc
    17881 gtgaagcctt tgcgaccatt gctgtcgcgg ctgataaagt cgacgccctg aaatctctgg
    17941 tgaataccta tcaggagatc ctgaaaaacg agctggcaga gaaagagaaa aatctggcct
    18001 tgttgctgga ctctgttgcg aacgataaag ctgccctgat tgcgaaatct cgcgatacct
    18061 ttattcgtct gctgaacgcc accccgaacg gtgtgatccg caattccgac gtggcaaaag
    18121 gtgtggtcga aacctccctg aacgtcggtg tggtgaccat gactgacaat aatgtagaaa
    18181 ttcactgtct gatccgttct ctgatcgaca gcggtaaaga ctacgtggtg agcatgctgg
    18241 attcgctggg taaactggct ggcgcgaaaa ccgaagcgaa aggtgcatat cctggctggc
    18301 agccggacgc aaactctccg gtgatgcatc tggtacgtga aacctatcag cgtctgttca
    18361 acaagacgcc gaacatccag attatccacg cgggcctgga atgtggtctg ttcaaaaaac
    18421 cgtatccgga aatggacatg gtttctatcg ggccaacaat caccggtcca cactctccgg
    18481 atgagcaagt tcacatcgaa agcgtaggtc attactggac actgctgact gaactgctga
    18541 aagaaattcc ggcgaagtaa ttatttgatt tgctgccgga tggcgtttaa tcgccttccg
    18601 gcagtttcat ccttcattat ccttcgataa aagccatccc tgtaaacgtt cgtcgcgggt
    18661 tgccacgttc aatctggtga tggaacattc gccgctgtga tttcagcgcc gcgctatttt
    18721 cctgttgctg ctgctccagc ctccaggcaa tcagcaatcg cgccagccgt ttgttagcat
    18781 gctggctgcg ctctgactga accttcacgc taataccgga tgccagatgc gtggcgcgta
    18841 ccgccaagtc ggttttattg acattttgac cgcccggccc cgacgaacgc agcgtgtcag
    18901 gataacgccc ggcttcggtt tccagcaccg ttaccgcgac gtcttgccgg gtaaaaacga
    18961 ccaacagaga agaacgctgc gccaatcggg tagccgcgtc cggggtgtgt aaatctggca
    19021 tccccactac gcgctgcatg ttgggtaaat ttgccgtggt gtgtaattgt tggatcgcta
    19081 aactctcgat ccacaggtca tcagatgcga tggtaaatac cgcatcggat aagggacgaa
    19141 tatatttgcc catgtaccat tccatttatg aataaaaatc aggaaatggc agacgaatcc
    19201 agggcaagaa aaaaagaatt aatcctggaa aggtctgcaa agagtcattg ataaaggatt
    19261 tgtcatgatt tacctccttg cagtttgtgg aatcagaaaa cgcgcacata ccaggcgggc
    19321 gttattttca ttgcaagctg gatttaatgt tgcggtttat atcgcatata aaaattagta
    19381 aaccacgctc cccggcattc aacgcgctgc tgcttaactg tctgaaaacc ataacgttca
    19441 aaaaagggtt ttgcggttat gctggcgtcc accgtaagtt cggattcaga cttaatccaa
    19501 ggttttaaca aagcactggc aaccccacgg cgggtgtatt cagggtcaac aaataacata
    19561 tcgatataat gttcaatgcg ggaaataaaa ccaaccggtt gtgcattaat gactgcaacc
    19621 cgcacttgtg atttcgcgag tttctccttc cagcgagatt cgtcaatctg cgcccaggca
    19681 gcaatttgtt gtggtgaata atgctgactg gctgtcatcg taaccgctct aatgaaaata
    19741 gcgcatagtt gctgaaaatc gccaggctga tagtttctta tttgtatgtt attcataata
    19801 taaattcaaa aacgcatgcg aaacgcttct gccattttcc caattttatg catttggttg
    19861 ttatctcgag ccagtttatg aggttcaggt ttcagaatgg caatgagcaa ccatgcttgc
    19921 tcatcaaacg caccctgaca atataccaaa tgcgcttcgt cattcgttct gctgaattgg
    19981 cgcaactgtg gcggaaatgg attattcaca tttgccagat gaatatgagc aactcgctca
    20041 aatttgatta atggccaggt aaacgagtcg tcgtagagtg catcgcgacc aaatatatct
    20101 ggcaaaacac cgtcacgctt ataggaaata aaatccgccg ttaacgcatc aagttcctct
    20161 gctgtaagtt gcaggcgaat aagttttgtt ttgaataccc gcatccttat tccttaaagt
    20221 cattgaaatc atcatccgtc atatcattaa gatattgttc agcgcggcgt gtaatttcct
    20281 ctaatcttgc agcacttgga cggaagcgcg cctttatggg ctttttctcc tcttgttcag
    20341 caagcatgtc cattaacgct tcgaatgcgc tggcacttaa gagatatcct gcggggcgat
    20401 tattagaaag aaccgcaacc ggttgatcaa taaagtattt agctgggttt ttacgtaact
    20461 cagtgatatt gaccgatttt tcagcgagaa ttcgatgcat acagtgatcc cctcataata
    20521 atgcacacca gaatagacat aatagtatac atcataatcc cagcaccagt tgtctttcca
    20581 tttgagggtc aagcaacgtc acatgcagcc ccaccagtcg cacaccgcgc ccaccgcggc
    20641 gttcatccca ggttttacgc gcggtggcga ttagatcagc tttattcagc cgcggccaga
    20701 cgtgctcctg ggtggtttgc tgaaaatcgt cgaactttaa tttcaccccc tggcgagcaa
    20761 tcagtaaatc aggttttacc tttgccagac ggcgttcaag ttccggatac agccgctcga
    20821 taatcgcttc acattcagac cagtgatgaa tatcttccgc catcgtgcgt tccacgccga
    20881 cggattttcg caaccgttcg ctgttaacat cgcgttcgtc aatcccctga ctacgctccc
    20941 acaaaatgcg gccaaatttg ccaaagcgtt taagcagcat caccagatca cacttttgca
    21001 catcaccgca ggtccgcagc cccatcgctt ccagttttgc cgccgagact ttgccgacgc
    21061 cggggatttt tgccagcggt aaggtttgca gaaatgccgg aacttctgcc ggagtaatca
    21121 caaactggcc gttgggttta ttcatgtcgg aggcgatttt ggcgagaaac tttaccggcg
    21181 caacgcccgc agacgccgtc agttgcagct cgttgaaaat cgtctggcgg atttcctggg
    21241 caatgagggt cgcagaaccg tggcaatgga cgctgtcggt gacatcgaga taagcctcat
    21301 ccagtgacaa cggctcaatg cgagaggtgt agcgcgagaa gatttcacgg atatgatttg
    21361 aggcttcttt gtaggcgtca aagcgccccg gaagcaaggt gagatgtggg cataatttga
    21421 gcgccatccc tgtcggcata gcgctgcgta cgccaaattt acgcgcggga taattggcgg
    21481 tgctgatcac cccccgacgt tcgcggctgc cgccaatagc aatagggata tcgcgcaggg
    21541 cgggattgtc gcgcatctcc actgcggcga aaaagcagtc catatccaca tgaatgattt
    21601 tacgcattac tcacctctca acactggtaa agtatacagt gatttcaggg tttgagaaat
    21661 gcgtaaagat tcagcattta acgcgttcgc tgcgaaagca cctgctgctt atccagcttt
    21721 tgcaccagcg gctgcaccac tttatcccca tgctgaccaa agtattgata cagcgtatcg
    21781 gacgcacttt tggtcagcac cataatctca atacgccgat tgcccgcgct ttgcggattt
    21841 ttggcatcca gcagcatctg gtccgccatt gcgcttacct gcatcacttt atcttccggc
    21901 attccggcct cttccagcac ccgacgagct gaaagcgcgc gatcgcccga aaggttccag
    21961 ttgttgtaga tattgttttt gtaggccatc gcatcggtat gcccggtaat aataattttg
    22021 ttatcgagcg agtcgaacac tggcgcgagc tccaccagca aagttttaaa gaacggcata
    22081 atctgcgcgc tgccgcgttc gaacatattg cgattctggt cgtctttaat cagcacgcgt
    22141 aatccctgcg ggacaatctc catctccaga ttcgcttcca tatgcgcatc acgggcaatg
    22201 gtgttgatac tggttgccaa ctcgccaagc tcggtggcgg atttcttctt cagcagcgcc
    22261 gttttctcat tcacatcacg cgcttttttc tccgtctctt ccggcaccgc cacggtggca
    22321 ggttttggcg gatgagacgg gctgatttta tttaacggcg acagcccacc accgttaaaa
    22381 atcgactgcc cgtgcagcgc ggcgataatg ctttcacgct cggatttact gacgctgttg
    22441 acaatcaact ttatcgtcgt caccaaaggg gccgagcgta tttccgaggt ttctgcccgc
    22501 tttaccctgg acgcgatgcc cggcaaacag atggcgattg acgccgacct taacgccggg
    22561 ttgatcaacc aggcgcaggc gcaaacccgg cgtaaagatg tcgccagcga ggccgatttc
    22621 tacggcgcga tggacggggc atcaaagttt gtgcgcgggg acgccatcgc cgggatgatg
    22681 attctggcga tcaacctgat cggcggtgtc tgtatcggga tcttcaaata caacctgagc
    22741 gccgatgctg ccttccagca atatgtgctg atgaccatcg gcgacgggct ggtggcgcag
    22801 atcccttccc tgctgctctc caccgcggcg gcgattatcg tcacccgcgt cagcgacaac
    22861 ggcgatatcg cccatgacgt gcgccaccag ctgctggcaa gcccgtcggt gctctacacc
    22921 gccaccggga ttatgtttgt gctggcggtg gtgccgggaa tgccgcatct gccgtttttg
    22981 ctgttcagcg ccctgcttgg ctttaccggc tggcggatga gcaaacgccc gcaggcagcg
    23041 gaggcggagg agaaaagtct cgaaacgctg acccgcacca tcaccgaaac cagcgagcaa
    23101 caggtcagtt gggaaacgat accgctgatc gagcctatca gtttaagcct cggttacaag
    23161 ctggtggcgc tggtggacaa agcccagggc aacccgctca cccagcgtat tcgcggcgtg
    23221 cgacaggtga tatccgacgg caacggcgtg ctgctgccgg agatccgcat tcgggaaaac
    23281 ttccgcctta agcccagcca gtacgctatt ttcatcaacg gcattaaggc tgatgaagcg
    23341 gatattccgg cggataaact gatggccctg ccctccagcg aaacctacgg cgagattgac
    23401 ggcgtgctgg ggaacgaccc ggcgtacggg atgccggtga cctggatcca gcctgcgcag
    23461 aaagcgaagg cgctgaatat ggggtatcag gtgatcgaca gcgccagcgt gattgccacc
    23521 catgtgaata agattgtacg cagctatatt cctgatttgt ttaactatga cgacatcaca
    23581 cagttgcata accgtttgtc gtcgatggca ccgcgcctgg cagaagactt aagcgcggcg
    23641 ctgaattaca gccagttgct gaaagtgtac cgcacactac tgaccgaagg cgtttccctg
    23701 cgcgatatcg tcaccatcgc caccgtgctg gtcgccagta gcgcggtgac taaagatcat
    23761 attctgctgg cggccgatgt acgcctggcg ctgcggcgca gcattaccca tccgttcgtt
    23821 cgcaagcagg agctgacggt gtatacgctg aataatgagc tggaaaatct gctcaccaat
    23881 gtggtgaatc aggcgcaaca gggcggcaaa gtgatgctcg acagcgtgcc ggttgacccg
    23941 aatatgctca accagttcca gagtacgatg ccgcaggtaa aagagcagat gaaagcggcg
    24001 gggaaagacc cggtgctgct ggtgccgccg cagttgcgtc ctttgctggc gcgctatgcg
    24061 agattgtttg cgccggggct gcatgtgctg tcgtataacg aagtgccgga tgagctggag
    24121 ttgaagatta tgggggcgtt gatgtaggta tccggcatcg gtgcacaacc gccggatgcg
    24181 gcgtgaacgc cttatccggc ctaccgatcc ggcacaggtt tgtaggcatg ataagacgcg
    24241 ccagcgtcgc atcaggcatc cgcgcacaat tgccggatgc ggcacaagtt tgtaggcatg
    24301 ataagacgcg ctagcgtcgc atcaggcatc cgcgcacaat tatgaaatga tacgctcccc
    24361 ggcactaaaa tccgttatgt tacccgccca atcaatggga tataaccctc gcgccacatc
    24421 gcgatggaac gttgagaatg gccaatcact cacttgcttt acccagccat gttttactgg
    24481 atttatataa atataatcaa catgatggcg atagtctttg gtattgcgga tggtgtgctc
    24541 ccaaaaacgt ggttgccaga tatttttcaa tccacaagca tgggtgaatt gctttttaat
    24601 ctcccgccag cgagaggaaa aatcatcatc gctttcaggt aatgtccaga tacagtgcat
    24661 atgctccggc aaaactaccc aggcattaat ttcaaaaggt ctggctcgct taactttaat
    24721 aatggcgtta cggagcgtct ggtactgggt agtcaaaagt tggcttcgac gattccgtaa
    24781 attcaccgtg aaaaaccagc ttcccccctt gaggtaataa cgacgatatt cagacatagc
    24841 gactcctttt tctcaacggt aagaagaaaa ggaaaggttg gcaatgaggt tgttttatat
    24901 cgggaaagcg gctcgcaaat attcgcgggc tcgtaggcat gataagacgc gccagcgtcg
    24961 catcaggcat cggtgcacaa ctgccggatg cggcgtaaac gccttatccg tcctacaaaa
    25021 tcgtctcttc cgtcaaaatc cggcgcgcgc ctaaaaaatg atcctgccag aaaggttcag
    25081 ctaaccgact tacccgaatg gtttccccgg tgcgcggtga ctcaataaac tgaccgtcgc
    25141 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aacagcaaat
    25201 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg gcggtagtga tacatctcat
    25261 tggccgtgcg cgggagcttc gcctcgagga tcttgttgta ggcataaaaa accaacccgc
    25321 tacagtcgaa gcctttatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    25381 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    25441 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    25501 attctggatg ctgttttatc tgttcgcggt tgtgctggcg cagcgccctt ctgctttcgg
    25561 cattgccttc cacaatatag ctggcctgct ttttcagatg ggtttggtac tgttttaata
    25621 aacgcgcacg gttttgcatc cgctggcggg cggcgtagct gaaagagata tgtgatgtgg
    25681 tggcgctggc aaatgaagaa aacggcaaac aaatcaacag taatcccgac aatacaaagg
    25741 atggaatcga cggcaaggac atactcaata atggctggtc atgctttaat gttgcagtgt
    25801 atcccggatc gtttctgaac aaacttaata aaccattatt ataaaattaa catatatatt
    25861 tcagtgaatt atgtaaatac atcagtttat tgaattgctc ataaattgcc gctgttgctc
    25921 aattgagcta caattcaagc tgaatatata tacagcacag gagatacccc aatggctgca
    25981 aatgcatttg ttcgcgcccg aatcgatgaa gatctgaaga atcaggcggc ggacgtactg
    26041 gccgggatgg ggctgaccat ctctgacctg gttcgcataa ccctcacaaa ggtcgcgcgt
    26101 gaaaaggcat tgccgtttga tttacgcgag cctaatcaat taaccattca atcaatcaaa
    26161 aacagcgaag ctggcgttgg tgttcataag gccaaagacg ccgatgattt atttgataaa
    26221 ttaggaattt aaatatgatt caaagggata ttgaatactc gggacaattt tcaaaggatg
    26281 taaaacttgc acaaaagcgt cataaggata tgaataaatt gaaatatctt atgacgcttc
    26341 ttatcaataa tactttaccg cttccagctg tttataaaga ccacccgctg caaggttcat
    26401 ggaaaggtta tcgcgatgct catgtcgaac cggactggct cctgatttac aaacttaccg
    26461 ataaactttt acgatttgag agaactggaa ctcacgcggc gctctttggg taaagcccgc
    26521 tgccgatgaa atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa
    26581 tgtgagcctt ccaaatccag actattccga tttgggatcc attttgccgt cctggcgtgt
    26641 gtaaccgttt tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt
    26701 gttgatcccg tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt
    26761 gagcaaagag tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga
    26821 agaacgtacg ctcgatctct acgtcaaaat gggtgagcaa tatcaactgc tcgacagcta
    26881 taaaatctgt aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag
    26941 cccggaaggg ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa
    27001 agcgattaat attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata
    27061 cctgatgatc cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat
    27121 tgatgagatc ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt
    27181 gagtatttac ccgttccgca tgaccgacgc taatatgaag cgccacaaat attccaactt
    27241 taaggacttc tgggagcaac tgaagccagg ctacgactac tttgagcaaa cccgtaagcc
    27301 accgaccgtt tctgtggtca atggccgtta cgtggtcagc aagccgttaa gccacgaagt
    27361 tgtgcaacca caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc
    27421 ctggcataat cttttgccag gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga
    27481 ccacatcatt cggtgtggtc tgcgagctga agtcgatttc tacatcctga tccagcaacg
    27541 ttgccacgcc aaacggcgcg cggcgggtga tccagtgtaa attagtcgag caatacgcca
    27601 ttacatagcg accgtccgaa agcagcatgt tgaaaacgcc cttcgcccgc agttcatccg
    27661 ccagtgaggc gatatattta aacactgccg ccatgttgcc cggcgtgcgc gggtaacgct
    27721 gcgttaattt atgcaggagc cagcaaaagg ctttttcgct gtcggtttca ccgaccgggc
    27781 ggaagttgct ggtttccagt gatttgtagc ccgtcagttg cccgttatgg gcataagtcc
    27841 agttacgccc ccataactcg cgggtaaacg ggtgagtatt ttccagcgcc acctcgcccc
    27901 gattagcctg gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacca
    27961 gtttggcgat aggtgagtta aagctgggtt gcggatcttt aaatgtgcga cagcctttac
    28021 cttcgtaaaa ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgca
    28081 ccagaccggt gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt
    28141 cgcacatggg taacctccac aacagcggga gcgcatggcc cccggcgaag attgatttaa
    28201 gaattttcag gtcggatgcg gcgtgaacgc attattcggc ctacggttcg cgtacagatt
    28261 tgtaggcatg ataagacgcg tcagcgtcgc atcaggcatc ggtgcacaat tgccggatgc
    28321 ggcgtaaacg tcttatccgg cctacgccag acttacttaa ccatctcttt ttcaatcaac
    28381 tggatcagga tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag
    28441 tgcggtacgc gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc
    28501 agggtgatca ctttcattcc cttctcacgc gccgctgcga tcgctttgat cacgtttgca
    28561 gagttaccgg aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg
    28621 taacgggaga aaatatcatt gaaaccgaaa tcgttaccga cgcaggaaat atgactaacg
    28681 tcagaaatag caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct
    28741 tcggcaaagt gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca
    28801 ccggctttaa agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg
    28861 tcatctttta aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga
    28921 tcctggtaca tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga
    28981 taccgccaaa agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg
    29041 cggataaaaa cagcaacaat gtgagctttg ttgtaattat attgtaaacg cattgctaaa
    29101 tgtttttaca tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg
    29161 gcttttcgtt atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt
    29221 ctatcaccgc gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct
    29281 cggcgttgct ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc
    29341 atttaacttt gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gcggtttccg
    29401 taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg
    29461 gtgggagggc gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca
    29521 gccgcgcctg acggctgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg
    29581 gatggcgaat gatttccaga tcacccatga actggcggat ctgccgccag agttgtgggc
    29641 gtaccttaaa gagcatcgct tcttcgcgat gatcatcaaa aaagagtacg gcgggctgga
    29701 gttctcggct tatgcccact ctcgtgtgct gcaaaaactc tccggcgtga gcgggatcct
    29761 ggcgattacc gtcggcgtgc cgaactcatt aggcccgggc gagctgctgc aacattacgg
    29821 cactgacgag cagaaagatc actatctgcc gcgtctggcg cgtggtcagg aaatcccttg
    29881 ttttgcgcta accagcccgg aagcgggttc cgatgcgggc gcgattccgg acaccgggat
    29941 tgtctgcatg ggtgaatggc agggccagca ggtgctgggc atgcgactga cctggaacaa
    30001 acgctacatt acgctggcac cgattgcgac cgtgctgggt ctggcgttta aactctccga
    30061 cccggaaaaa ttactcggcg gtgcagaaga tttaggcatt acctgtgcgc tgatcccgac
    30121 caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtac cgttccagaa
    30181 cggaccgacg cgcggtaaag atgtcttcgt accgatcgat tacattatcg gcggaccaaa
    30241 aatggccggg caaggctggc ggatgctggt ggagtgcctc tcggtaggcc gcggcatcac
    30301 actgccttcc aactcaaccg gcggcgtgaa atcggtagcg ctggcaaccg gcgcgtatgc
    30361 tcacattcgc cgtcagttca aaatctctat cggtaagatg gaagggattg aagagccgct
    30421 ggcgcgtatt gccgggaacg cttatgtaat ggatgctgcg gcatcgctga ttacctacgg
    30481 cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc actgtaccca
    30541 ccgcgggcag cagtcgatta ttgatgcgat ggatattacc ggcggtaaag gcattatgct
    30601 cgggcaaagc aacttcctgg cgcgtgctta ccagggcgca ccgattgcca tcaccgttga
    30661 aggggctaac attctgaccc gcagcatgat gatcttcgga caaggagcga ttcgttgcca
    30721 tccgtacgtg ctggaagaga tggaagcggc gaagaacaat gacgtcaacg cgttcgataa
    30781 actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg
    30841 cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat gccactaaac gctactatca
    30901 gcacctgaac cgcctgagcg ccaacctcgc cctgctttct gatgtctcga tggcagtgct
    30961 gggcggcagc ctgaaacgtc gtgagcgtat ctcggcacgt ctgggtgata ttttaagcca
    31021 gctttacctc gcctctgccg ttctgaagcg ttatgacgac gaaggccgta atgaagccga
    31081 tctgccgctg gtgcactggg gcgtacagga tgcgctgtat aaggctgaac aggcgatgga
    31141 cgatttactg caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg tagtgatctt
    31201 cccgaccgga cgtcattatc tggcaccgtc tgacaagctg gatcataaag tggcgaagat
    31261 tttacaagtg ccgaacgcca ctcgctcacg tattggtcgc ggtcagtacc tgacgccgag
    31321 cgagcataat ccggttggtt tactggaaga ggcgttggtg gatgtgattg ccgccgaccc
    31381 gattcatcag cggatctgta aagatctggg taaaaacctg ccatttaccc gtctggatga
    31441 actggcgcac aacgcgctgg cgaaggggct gattgataaa gatgaagccg ctattctggt
    31501 gaaagcggaa gaaagccgtc tgcgcagtat taacgttgat gactttgatc cggaagagct
    31561 ggcgacgaag ccgataaagt tgccggagaa agtgcggaaa gttgaagccg cgtaattgaa
    31621 taacggagcc gaaaggctcc gtttctttat ccgctaatta tttaaaatta aagccatccg
    31681 gatggttttc caggctgccg gtcaacgccg cgaacagcac cgttttacca tcaatcgaaa
    31741 gcgcatcgtt cacattcaac caggtgagtt tctcttgcga cgttttctca tcaatagtcg
    31801 agaacagccc cgtcatctga ttagatttct cggaccacat cacagcgata cgttgcgagc
    31861 cacagtcatg cggtttgcac gcgctcatca cctgatacgt ctcatctccc aatgtcacgg
    31921 tttgtgcggg agtataagta ccgcctttca tcacccatgc aggtagctta tgcccttgca
    31981 ccatctgatt aaacgcagct ttggtggttt cgccctttgc caggctgcta atggttaaat
    32041 catcctgcgc cattgcactg gtggcgatga ccagagcggc gactgtcgtt attgccttaa
    32101 acatcattcc tcccgagctt atcctgccca tgttattaac ctcctaaagc ctggcgataa
    32161 cacgataatt tgcaaatcag atatttccaa tatcagcatt tgttatcaat ttattctgcg
    32221 ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc cttcgcataa aggagttgta
    32281 caccgtgcct ggtttgaaga ttacgctttt gcagcaaccg ttggtgtgga tggatggtcc
    32341 tgccaacctg cgtcattttg atcgtcaact ggaaggtatt accgggcgcg atgtgatcgt
    32401 tctaccggag atgtttacca gcggctttgc catggaagcg gcagcttcgt cgctagcgca
    32461 agatgacgta gtgaactgga tgacagctaa agcgcagcag tgcaatgcgc tgattgcagg
    32521 cagtgttgca ttacaaacgg agtctggttc agttaaccgc tttttgctgg ttgagccggg
    32581 cggcacggta catttttatg ataagcgtca tctgttccga atggctgatg agcatctgca
    32641 ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc ggctggcgta ttttgccgct
    32701 ggtgtgctac gacttacgtt ttcctgtgtg gtcgcgcaat ctcaacgatt atgacctcgc
    32761 cctgtacgtc gccaactggc ctgctccgcg ctctctgcac tggcaggcat tgctgacggc
    32821 ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat cgcgtcggca gcgatggcaa
    32881 cggctgccat tatcgcggtg acagccgggt gattaatcca caaggcgaga ttatcgctac
    32941 tgccgacgcg catcaggcaa cgcgcattga tgcggagctg tcgatgatgg cattgcggga
    33001 atatagagaa aagtttccgg catggcagga tgcggatgag tttaggttgt ggtgagtggg
    33061 ggttgtgttt agggccgggg agagtcgggg tatgactacg aaagcccgct ctcctgcgac
    33121 cacttttatc ataggagtgt ggaagtgctt ttccatcaat cgcgatgacg tctttgtcat
    33181 ttgatgaatg gcagtcacgc atccagttaa taaagcactc gtgaaatttt gcaggactga
    33241 tacaggatac aactctggca atggtatcgt
//
LOCUS       DAZGBC010000037        32642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGBC010000037 DAZGBC010000000
VERSION     DAZGBC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.037"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..527
                     /gene="uspC"
                     /locus_tag="ACHK1J_001776"
     CDS             99..527
                     /gene="uspC"
                     /locus_tag="ACHK1J_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO7911056.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGN"
     gene            complement(534..1958)
                     /gene="otsA"
                     /locus_tag="ACHK1J_001777"
     CDS             complement(534..1958)
                     /gene="otsA"
                     /locus_tag="ACHK1J_001777"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO7911057.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1933..2733)
                     /gene="otsB"
                     /locus_tag="ACHK1J_001778"
     CDS             complement(1933..2733)
                     /gene="otsB"
                     /locus_tag="ACHK1J_001778"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO7911058.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2900..3886)
                     /gene="araH"
                     /locus_tag="ACHK1J_001779"
     CDS             complement(2900..3886)
                     /gene="araH"
                     /locus_tag="ACHK1J_001779"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO7911059.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3901..5415)
                     /gene="araG"
                     /locus_tag="ACHK1J_001780"
     CDS             complement(3901..5415)
                     /gene="araG"
                     /locus_tag="ACHK1J_001780"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO7911060.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5485..6474)
                     /gene="araF"
                     /locus_tag="ACHK1J_001781"
     CDS             complement(5485..6474)
                     /gene="araF"
                     /locus_tag="ACHK1J_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO7911061.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7271..7774
                     /gene="ftnB"
                     /locus_tag="ACHK1J_001782"
     CDS             7271..7774
                     /gene="ftnB"
                     /locus_tag="ACHK1J_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO7911062.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7853..8104)
                     /gene="yecJ"
                     /locus_tag="ACHK1J_001783"
     CDS             complement(7853..8104)
                     /gene="yecJ"
                     /locus_tag="ACHK1J_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO7911063.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8219..8305)
                     /gene="azuC"
                     /locus_tag="ACHK1J_001784"
     CDS             complement(8219..8305)
                     /gene="azuC"
                     /locus_tag="ACHK1J_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO7911064.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8568..8891
                     /gene="yecR"
                     /locus_tag="ACHK1J_001785"
     CDS             8568..8891
                     /gene="yecR"
                     /locus_tag="ACHK1J_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO7911065.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9062..9559
                     /gene="ftnA"
                     /locus_tag="ACHK1J_001786"
     CDS             9062..9559
                     /gene="ftnA"
                     /locus_tag="ACHK1J_001786"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO7911066.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9597..9836)
                     /gene="yecH"
                     /locus_tag="ACHK1J_001787"
     CDS             complement(9597..9836)
                     /gene="yecH"
                     /locus_tag="ACHK1J_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO7911067.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10027..11238
                     /gene="tyrP"
                     /locus_tag="ACHK1J_001788"
     CDS             10027..11238
                     /gene="tyrP"
                     /locus_tag="ACHK1J_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO7911068.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11300..11965)
                     /gene="yecA"
                     /locus_tag="ACHK1J_001789"
     CDS             complement(11300..11965)
                     /gene="yecA"
                     /locus_tag="ACHK1J_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO7911069.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAYPQEQAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12161..12247)
                     /locus_tag="ACHK1J_001790"
     tRNA            complement(12161..12247)
                     /locus_tag="ACHK1J_001790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12211..12213),aa:Leu,seq:taa)
     gene            complement(12260..12333)
                     /locus_tag="ACHK1J_001791"
     tRNA            complement(12260..12333)
                     /locus_tag="ACHK1J_001791"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12299..12301),aa:Cys,seq:gca)
     gene            complement(12388..12463)
                     /locus_tag="ACHK1J_001792"
     tRNA            complement(12388..12463)
                     /locus_tag="ACHK1J_001792"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12428..12430),aa:Gly,seq:gcc)
     gene            complement(12615..13163)
                     /gene="pgsA"
                     /locus_tag="ACHK1J_001793"
     CDS             complement(12615..13163)
                     /gene="pgsA"
                     /locus_tag="ACHK1J_001793"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO7911070.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13220..15052)
                     /gene="uvrC"
                     /locus_tag="ACHK1J_001794"
     CDS             complement(13220..15052)
                     /gene="uvrC"
                     /locus_tag="ACHK1J_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO7911071.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15049..15705)
                     /gene="uvrY"
                     /locus_tag="ACHK1J_001795"
     CDS             complement(15049..15705)
                     /gene="uvrY"
                     /locus_tag="ACHK1J_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO7911072.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16001..16177
                     /gene="yecU"
                     /locus_tag="ACHK1J_001796"
     CDS             16001..16177
                     /gene="yecU"
                     /locus_tag="ACHK1J_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO7911073.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16164..16388
                     /gene="yecF"
                     /locus_tag="ACHK1J_001797"
     CDS             16164..16388
                     /gene="yecF"
                     /locus_tag="ACHK1J_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO7911074.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16456..17178)
                     /gene="sdiA"
                     /locus_tag="ACHK1J_001798"
     CDS             complement(16456..17178)
                     /gene="sdiA"
                     /locus_tag="ACHK1J_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO7911075.1"
                     /translation="MQDKDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQLLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(17408..18160)
                     /gene="tcyN"
                     /locus_tag="ACHK1J_001799"
     CDS             complement(17408..18160)
                     /gene="tcyN"
                     /locus_tag="ACHK1J_001799"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO7911076.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGESKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(18157..18825)
                     /gene="tcyL"
                     /locus_tag="ACHK1J_001800"
     CDS             complement(18157..18825)
                     /gene="tcyL"
                     /locus_tag="ACHK1J_001800"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO7911077.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(18840..19826)
                     /gene="dcyD"
                     /locus_tag="ACHK1J_001801"
     CDS             complement(18840..19826)
                     /gene="dcyD"
                     /locus_tag="ACHK1J_001801"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO7911078.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNVLGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(19931..20731)
                     /gene="tcyJ"
                     /locus_tag="ACHK1J_001802"
     CDS             complement(19931..20731)
                     /gene="tcyJ"
                     /locus_tag="ACHK1J_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7911079.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(20819..21370)
                     /gene="fliZ"
                     /locus_tag="ACHK1J_001803"
     CDS             complement(20819..21370)
                     /gene="fliZ"
                     /locus_tag="ACHK1J_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO7911080.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPSSDIY"
     gene            complement(21416..22135)
                     /gene="fliA"
                     /locus_tag="ACHK1J_001804"
     CDS             complement(21416..22135)
                     /gene="fliA"
                     /locus_tag="ACHK1J_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO7911081.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22455..24167)
                     /locus_tag="ACHK1J_001805"
     CDS             complement(22455..24167)
                     /locus_tag="ACHK1J_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO7911082.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSSTLKLTSFNVNGKGAVDNAKATEADLTAAGFSQGAVVSGNSTWTKSTVTTFNA
                     ATATDVLASVSGGSTISGYTGTNNGLGVAASTAYTYNATSKSYSFDATALTNGDGTGA
                     TTKVADVLKAYAANGDNTAQISIGGSAQDVKIASDGTLTDVNGDALYIGSDGNLTKNQ
                     AGGPDAATLDGIFNGANGNAAVDAKITFGSGMTVDFTQASKNVDIKGATVSAEDMDTA
                     LTGQAYTVANGAQSFDVAAGGAVTATTGGATVNIGADGELTTATNKTVTETYHEFANG
                     NILDDDGAALYKAADGSLTTEATGKSEATTDPLKALDDAIASVDKFRSSLGAVQNRLD
                     SAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLS
                     LLQG"
     gene            24433..25839
                     /gene="fliD"
                     /locus_tag="ACHK1J_001806"
     CDS             24433..25839
                     /gene="fliD"
                     /locus_tag="ACHK1J_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO7911083.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            25864..26274
                     /gene="fliS"
                     /locus_tag="ACHK1J_001807"
     CDS             25864..26274
                     /gene="fliS"
                     /locus_tag="ACHK1J_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO7911084.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            26274..26639
                     /gene="fliT"
                     /locus_tag="ACHK1J_001808"
     CDS             26274..26639
                     /gene="fliT"
                     /locus_tag="ACHK1J_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO7911085.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26717..28204
                     /gene="amyA"
                     /locus_tag="ACHK1J_001809"
     CDS             26717..28204
                     /gene="amyA"
                     /locus_tag="ACHK1J_001809"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7911086.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28238..28651)
                     /gene="yedD"
                     /locus_tag="ACHK1J_001810"
     CDS             complement(28238..28651)
                     /gene="yedD"
                     /locus_tag="ACHK1J_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO7911087.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKAGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            28838..30043
                     /gene="yedE"
                     /locus_tag="ACHK1J_001811"
     CDS             28838..30043
                     /gene="yedE"
                     /locus_tag="ACHK1J_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO7911088.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            30040..30273
                     /gene="yedF"
                     /locus_tag="ACHK1J_001812"
     CDS             30040..30273
                     /gene="yedF"
                     /locus_tag="ACHK1J_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO7911089.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30382..31050
                     /gene="yedK"
                     /locus_tag="ACHK1J_001813"
     CDS             30382..31050
                     /gene="yedK"
                     /locus_tag="ACHK1J_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO7911090.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            31161..31640
                     /gene="yedL"
                     /locus_tag="ACHK1J_001814"
     CDS             31161..31640
                     /gene="yedL"
                     /locus_tag="ACHK1J_001814"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7911091.1"
                     /translation="MFTIKTDDLTHPAVQTLVAYHISDMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGMGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAQD
                     RCLHRLSLETGTQAGFTACHHIYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELL"
     gene            complement(31778..>32642)
                     /locus_tag="ACHK1J_001815"
     CDS             complement(31778..>32642)
                     /locus_tag="ACHK1J_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO7911092.1"
                     /translation="AYDKTGSITIEKSQGEGTLPIRHKLEFISTNIAELLDKLTKITD
                     ARLCKGFSDWASSVKEGASNDFKENVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLP
                     EWIEMLSLVYNELDSIQVPESCKELEVDFNNLTEFPQVPDGITLISVNNNLISHIDSF
                     PPKAKIISICHNKLSEIPALPDTAKVFDCSENNIKEIRWFPKNLKEAYIEYNKIEVIP
                     AIPGNLKLLFMECNPIKEAFLMPWTLTDITYEISQRKYIVTNPDDYDKYSDMVKKYVI
                     DGEELIIKYYM"
BASE COUNT         8173 a         8308 c         7750 g         8411 t
ORIGIN      
        1 ctaatattta cctgtttgac tgagttggga ttgcgtcgtt tctccattag gagtaaagct
       61 ttaatgtcac ctgaagttca cagaataagg aacaggttat gagctatagc aatattcttg
      121 tcgctgttgc ggttacaccg gaaagtcagc aactgctggc aaaagcggta tctatcgcca
      181 gaccagtaaa gggacacatc agtttaatta ctctcgcttc cgacccggaa atgtacaatc
      241 aattagctgc gccgatgctt gaagatttac gcagcgtgat gcaggaagaa acgcaaagct
      301 ttcttgataa gttaattcag gatgcggggt atcccgttga caagacgttt attgcctacg
      361 gtgagttaag cgaacatatt ctggaagttt gtcgcaaata tcatttcgat ttggtgattt
      421 gcggtaatca caatcacagt ttcttttcgc gagcatcctg ctcggcgaaa agagtgattg
      481 cctcaagtga agtcgatgtg ctgttagttc cacttacggg gaattaaccg ctcctacgca
      541 agcttaggaa aggtagcaac tttatcgcgc tgctggcttt ccgcgcttcg cggaactatc
      601 tgctttaggt cgctgatgaa gcactcctgc cagtggttaa tatcgttttt cacgataacg
      661 tccagcattt ctgcatgacg ggaaatacgt tccgccagcg acatagtcaa tgcacgatcc
      721 agcgcagctg caacttcgtc acgatcgtag ggattaacaa ttaacgccga cgttaactcg
      781 tttgccgctc ccgcaaattg cgaaagaaca agaacgcccg gattggctgg gtcctgagcg
      841 gcaacatact cttttgccac caggttcatc ccgtcacgca gtggcgtcac taagcccacg
      901 tcagagtagc ggaatatttt catcagtaat ttacggtcaa aatgctgatt caaataataa
      961 agaggcgtcc agcctaattg cccgtattta ccattaattc gtccagcttc attttcgagc
     1021 tgatgacgaa tatcctgata ggcttgcaca tcaccacgcg acgttggtgc aatctgggta
     1081 taacgaattt taccatgatg ctgcggatat ttttccagca acgcttcata ggcgagaaaa
     1141 cgctctggca aacctttgga ataatccagc cgttcgacag aaaagatatt ttgtacgttt
     1201 ttcagttccg ctttaagttg cgccagtttt ggcggcagtg gcccggcagc ctgtttggct
     1261 atttcttttg gctcaatgcc aatcgggtaa acttctgttc gaaacgcttt gccccaggct
     1321 gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt tagaaaggca atccaggaac
     1381 gccagacgat cgttttctgt ctggaaaccc agcaaatcat aatcacaaag ctgttcaagc
     1441 aaggtgtcat atgtcggcag cgcgttgaag atttccggtg tcgggaaagg aatatgcaga
     1501 aagaaaccaa tgcgattatt cactccccgt ttgcgtaatt catgcgcaaa tggcaacagg
     1561 tgataatcgt ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc
     1621 agcaacgcat ttacgcgtaa atagccgtcc caggcaggac gctgaaattg caccagatcg
     1681 aggcgataat gaaaagcagg ccagagaacg gcattggaga attggttgta gtattcgtca
     1741 aggtcctgtt cgctgaggtt aaaagaggcc cacgtaatgt tacctttttt cacctttttt
     1801 agcggctgat cctcattccc tgtttcacca ctccagccaa accacagtcc gcctgcggct
     1861 ttcagtgccc ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg ctcatctggt
     1921 ggtgcaatcc ggttagatac tacgactaaa cgactcatag tcatcactcc tgttattttc
     1981 tcttttttgt tgtaatgcgg tggttatcat ttcaagccag ctccagacat ccggtacgcc
     2041 cgccagtcgc catgatgcct gagttgcgcc tgtgccaatt tttactgaca ttccgcccag
     2101 tcggttaacg actgcgaagc cagattcatc ggttaaatca tcgcccagaa atacgggcgt
     2161 tcgcccgata aagggagctt cctgcataaa ggcagctatc gcctcacctt tactggtacc
     2221 tctcggtttg atctcgacaa cacactttcc ctgctgtaac gccatttgtg gccagatctg
     2281 agtaatacgt tgcgctaatg tcattaatgc gtcttcatgc tgcggagcct gacgataatg
     2341 cagcgcaaaa gccatccctt tcgtctccag ctccgcgccg ggatactggg cgatgaccgt
     2401 atgcagttgc acgctaatat cacgcgcaat cgcatccggc agatgaacga tatgcgtttt
     2461 accattgatg tcacggcgct ccgccccatg cacgcccgct aacgggaagc gataaggttt
     2521 tgccagtgcg tccagctcca ccattgagcg ccctgatatc aatgccaatg caccatcact
     2581 tgcggttgcc agtagctgaa gtccttgcag aatattgtca ggtacgacga cctgatcggg
     2641 atgcggtttg atttccgcca gcgttccatc gagatcaaaa aaccaggcat atttcgcgga
     2701 tagttcaggg gtttcggtta acggttctgt cacccggttc tcctccttct cttttcttat
     2761 tgttttgcgc agaacgcgct ttacagacat gtaagtatag acagtgtgac gggggtcgcc
     2821 atttgcaacg aagaacagcc aaccgttgtg gcggctggct gtggtgggaa aaaacgttag
     2881 attgttgtgg aaaaaagcat cagacagtgc gtttcgcttt ttgcttgtaa cggtcgaaga
     2941 tcaccgctgc cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa
     3001 gcaggttcat ggcgttttcc acggtgccta aaattaagat acccgccacc acatatgaga
     3061 tttttccgat gccacctttc agagaaacgc cacctaaaac gcaggcggag ataacaataa
     3121 gctcataacc aatcgacgtc attggctgcc cactagtcat acgtgaagcc agaataattc
     3181 cggctatcgc agataccagg ccagagagaa caaagataat aattttggtg cgaacaaccg
     3241 gtacacccgc cagacgcgca gcctcttcgt tcccgccaat cgccagggta ttacgaccaa
     3301 aggtggtttt attcagcagc aaaccaaaga taatcagaca cgcgacggtg agccagattg
     3361 gcgcaggcag accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac
     3421 cgaccgcttt accgtcggaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca
     3481 gtgtcgtgat cagagcattt attttcagtt tggcgataac aaagccattg accaggccac
     3541 agagaatgcc aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga
     3601 taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt
     3661 caccggaagc aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca
     3721 ggcccaaccc tttcatatta atgaaggtgg caaagttcgg gacaaaaatg gcacaggcga
     3781 taaaaagcac cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga
     3841 atgacgactt aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac
     3901 tcaggcaact gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc
     3961 ctgctcgtgt aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc
     4021 aacgccgagg acttcaggta agtcgctgga ggcaaacagc accgccacgc cctgcgccgc
     4081 cagcgcataa attacgttat atatttcgtg cttagcgcca acatcaatgc cgcgcgtagg
     4141 ttcatccagc aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg
     4201 ctgatttccg cctgagagat tcatgatcag ttgctccgcg cccggcgttt tgatgttgag
     4261 cgaacgaatg tggtgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc
     4321 gagcacatgt ttacgtctgg cactgatgtt gatattgtcg cgaacggagt gcacaggaat
     4381 aatgccttcc gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct
     4441 cggtttacga atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt
     4501 cccgccaaac atgcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc
     4561 aacaatttca ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac
     4621 agcatcaaga cgcaggcgct cctcgccata actacgcggt tgccagccgt agatatcgcc
     4681 aatgtcgcgc ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt
     4741 aaaggttttg acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat
     4801 ttcttccata cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat
     4861 aacgcggaaa agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc
     4921 gataatttta gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat
     4981 ggagagatat ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa
     5041 acccgcctca taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc
     5101 gagatagatg ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat
     5161 cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat
     5221 cactacagaa cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt
     5281 tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat
     5341 atccgtcagc gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg
     5401 ggtagactgt tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat
     5461 gcagagggga atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc
     5521 tttaaagttg tcacgcgtga tcagcaccac gtcggtaact tcggtgaatt tggtcggttc
     5581 aacacctttt gctacccagt tgtaaagcat ttcgctggac ttataaccat gtacgtccgg
     5641 gcttggcaac agggaaccgt agaagccggt tgcctgtgcc ttagacagtt cgctcaccgc
     5701 atccacaccg ttaataccga taccgatgat atcggccgct ttaaagccct gaccttccgt
     5761 cgcgcgtacg ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac
     5821 ttccggatgt tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga
     5881 tttggtaggt acctgataaa tttgtttttc cgggaatcct gcagctttca gcgcatccat
     5941 agaacccgta gtacggcgac gagccgtatc cagttcgttg gaggtaatcg ccatcaccgc
     6001 gctttctttg acatcccagc cacgtttctg catctcttta tacagttcct ggccctgacg
     6061 ttcgccaatt ttggtcgccg ccatcatcac cagaggaacg gtatccattg gcttaccttt
     6121 ggcgttaaca aactggtcat ccacggcaat gactttcata tcgtagccac gcgccttcgc
     6181 cacgatggca gagccgagtt tcgggtccgg agtacaaata acgaaacctt ttgcgccact
     6241 ggcggccagg ctatcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat
     6301 aacctcaaac cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca
     6361 cggctcttcc ggttgcttca ccaggaaacc gagcttcagg ttctccgcca tagcggattg
     6421 tgacataacg gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct
     6481 ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat gacattcgtt aaattaatcg
     6541 gaaaacaaag cattaccttt taactaaaag ataagtgact gtgttgacat agttttagcg
     6601 agaaattaat tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca
     6661 taaatttagc gggaaaagac ataaggaaaa gccaatttgt cagacaaatt gttgaatgca
     6721 cagcagatta atccataaga ttagcctgga aatccttgtt gtctttggta cccatgcggg
     6781 atgtcttctt tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt
     6841 gtaataacca caactcaaag atgtggaaaa tgcgcgtcat tcatttcgcc attaattatc
     6901 actgtgctca ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa
     6961 cgttttataa aatcatcact tcatcatgaa ttcaaattca tcgattaata tcaacaagat
     7021 acaaaaagca ctatcattaa aattcattgc agttacattg atctcatcaa tgaaatgtaa
     7081 aaatatataa acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt
     7141 tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt
     7201 ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc tggatataag
     7261 gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga
     7321 gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa
     7381 cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat
     7441 gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg
     7501 tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg
     7561 ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt
     7621 caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggcctgttgc tgcaaaccat
     7681 tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca
     7741 tgttctgaat gtcgtgtcgc atcagctgca ttgatcatac atcggcgcta atgcattgcg
     7801 ccgatgaagt ttttgagaaa ccgctgcctc atctttttga agcagcggtt ttttaatggg
     7861 attcgccttg tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca
     7921 tggtgaccgg gtctgcggcg gcgggatgat gagtaaagtc gatattcttc agctggctgg
     7981 acattttttc gcgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga
     8041 taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg
     8101 acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag
     8161 cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt
     8221 atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac
     8281 ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat
     8341 tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt gatgcaaatc
     8401 acataaatac ccctttaatg ttataaaaat gataaccaaa aaacagcccc cctatttctg
     8461 acacctacag atggcaggaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc
     8521 gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga
     8581 ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga
     8641 gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc
     8701 atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag
     8761 gctataccca cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt
     8821 ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg
     8881 tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa
     8941 tgcgttttat tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat
     9001 aataaaataa caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat
     9061 catgctgaaa ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc
     9121 ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc
     9181 cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta
     9241 cctgactgat accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata
     9301 ttcctcactt gatgaattat tccaggaaac ctataaacac gaacaattaa tcacgcagaa
     9361 aattaacgaa ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct
     9421 gcaatggtat gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa
     9481 attaagcctg gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac
     9541 cctcgacaca caaaactaat gctctccggc ggcaggtttt ctgccgccgt ttcaaatcag
     9601 tgacggcaaa tcttactctg atcggtcgaa aaaccttctt ctgaaggtat aaatttgcct
     9661 tttgctgcca gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaagtgtga
     9721 aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat
     9781 tgctcgcctg attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt
     9841 cctcctcaaa aaaaagccta gcgtagcgat taccgcttat gaagactttg cgccagcgca
     9901 ggactgaatg cttttattgt acatttaaat ttacaccata tgtaacgtcg gtttgacgaa
     9961 gcagccgtta tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa
    10021 gaaagcgtga aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc
    10081 gcaggcatgc tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tactttaatc
    10141 ttgttgattg ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac
    10201 cagcatgttc cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac
    10261 ggtcaatggc tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcggcatac
    10321 atcagcggtg ccggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg
    10381 tcggcaaccg ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga
    10441 acatcgctgg tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg
    10501 gtgatgctgg tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg
    10561 caacaggggc tggcactgtc tgcaatcccg gtgattttta cgtcgtttgg ttttcacggt
    10621 agcgtgccga gtattgtcag ctatatggat ggcaacattc gtaagctacg ctgggtgttt
    10681 ataaccggta gtgcgatccc cctggtggca tatattttct ggcaggtggc gacgcttggc
    10741 agcattgatt caacaacctt tatgggattg ctggctaatc atgccggatt aaacgggctg
    10801 ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg agctggcagt gcatttattt
    10861 gctgatttag ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg
    10921 gctgatttgt ttcagcgttc aaataccgtg ggtggaagat tgcaaactgg tgcaattacc
    10981 tttctgccgc cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt
    11041 tacgccggtg tggcgctggc ggtactggca ttgattatcc cttcgctgtt gacctggcaa
    11101 agcagaaagc acaatcctca agcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg
    11161 gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg
    11221 ttaccagaag tggggtgatt agatagcctc aaattcctta ttgggtgcca gaattaacgc
    11281 tgacacccaa tttgacttct taatgcaggc agcactgctt aaatttctta ccactaccgc
    11341 acgggcaagg atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt
    11401 gttcctgcgg atacgccatc cagtatgcat gtaaatcaag cgccgccagt cgaatggcat
    11461 ctacgctctc ttcaaacgct tctggcgaca tcttctctac ccgctcgaag ttttcctcag
    11521 taccgtgcag cgctatcgcc tccagcgctg gttttaacga atcgggcaat gttgaccagt
    11581 cagaaagtgc cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc
    11641 tgccatcaac ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac
    11701 gctctgcggt atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct
    11761 ctgacgccca gcgcggcaca tagtcagccc caccccacac ggcaaccagc cactgtgccg
    11821 gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat
    11881 caaggatggc gtggtcagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac
    11941 tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc
    12001 tatattacat gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa
    12061 atcaaggagg gtgtaacgac aagttgcagg cacaaaaaaa ccacccggag gtggtttcac
    12121 gacactgctt attgctttga ttttattctt atctttccca tggtacccgg agcgggactt
    12181 gaacccgcac agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca
    12241 tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg
    12301 caatccgcta cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca
    12361 tccgcttgag gccgatgtct tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc
    12421 cgaccttggc aaggtcgtgc tctaccaact gagctattcc cgcattcatc aagcaatcag
    12481 ttaatcactt gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc
    12541 tacttacctg gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa
    12601 ttgcgccgaa acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac
    12661 attgaccaca gagtcagtac cgcagccaca aagaaaagtg caatgccggc gtactcaacc
    12721 caaatgttcg gacgccacag cagccaggcc aacgccacca tctgggcagt ggttttcact
    12781 ttcccaatcc aggagacagc cacgctactg cgtttaccca actccgccat ccattcgcgt
    12841 agcgcagaaa taataatttc acgggcgatc atcgttgccg ccggtaaggt cacccaccag
    12901 ctgtggtaat gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgctaca
    12961 gggtcaagga aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg
    13021 aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg
    13081 acaggcagat aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc
    13141 aacgtaggga tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc
    13201 ctatgttgct acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt
    13261 gcgaaatacc cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac
    13321 cgcccatata tttcaacaac atttgccggc gttttggccc gacgccttca atggtttcca
    13381 gggaactggt atttttgacc ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat
    13441 gtgattcatc gcgaatatgc tggataacat gcagcgcggg agaatctggc ggcaaactga
    13501 atccctcacc ttccggctca aagaacagcg tttccagccc agccttacga tctgctcctt
    13561 tggcaacgcc aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaaa
    13621 catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat aagtatcaca tccgggatct
    13681 tactgtcgtc gatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat
    13741 catcgcccgg cgtgatgcca gtaatgttat agcgccgata ctccgcacgc agcgggccgt
    13801 tagcatcaaa caccacacag gaagcgacgg tttgttcacc catggtatgg ctgatgtcaa
    13861 agcactccat ccgcttcact tccggcaatt tcaacacgct ggcaagtgct gtaagccgct
    13921 ggtgaacggt agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg
    13981 cgagtttcag gtaacgcgcc ctatcgccac gaggtttggt ttgaacatta atcttgcgtc
    14041 ccgccagttc tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca
    14101 ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa atagaactgt cctacgaagg
    14161 tttccaccac ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc
    14221 caagcacttt gccctgacga atgaacaata cgtggacaca agccatgccc gcatcgaacg
    14281 ccacaccaat aacgtcaagg tcgtcgccgg tattggaaac aaactgtttt tcggtgacgc
    14341 gtcgcaccgc ctgaatttgg tcgcgaatac gtgccgcttc ttcaaactcc agattctggc
    14401 tggcagtttc catacgacta atgagttgcg taagcacctg atcatctttg ccagacaaaa
    14461 acaggcgcac atactcgacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc
    14521 acggtcccag acagcgccct atctgatatt gcagacacgg acgcgagcga ttgcgataaa
    14581 cactattttc gcactggcga atggggaaaa tcttttgcag tagcgccagt gtttcacgta
    14641 cggcatagcc attcgggaac gggccgaaat attcaccttt ggcatgcttt gcaccacgat
    14701 gcatcgccag acgcgggtgg gtatcaccac tcaggaagat aaaaggatat gatttatcat
    14761 cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat gtagttgtgt tccagcaaca
    14821 gcgcttcggt ttctgtatga gtaaccgtta catcaatttg ctggatctgg gcgaccagcg
    14881 cttcggtttt gcgcgaagcg aggttgctac ggaaatagct ggaaagccgt tttttcaggt
    14941 ctttcgcttt gccgacatag ataaccgtac caccagcatc gtacatgcga taaacgcctg
    15001 gctggctggt tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga
    15061 taatgtctcc gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc
    15121 atgaatgttt agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag
    15181 attgagctgt tctgagatct cattgacctt ctggcccttg gtgatcatca gcataatctg
    15241 caattcacgt tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct
    15301 taacgccatt tgttgagcga tgtcagaagc aatgtaacgc tgccctgaat agacagaacg
    15361 gatcgcactc acgacttcct gcggagccgc gcctttgctg aggtagcccg cagcaccggc
    15421 ctgcatgact ttcgctggta aagggttttc tgtatggacg gtaagcatga tgattttgac
    15481 atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact
    15541 catgtccatt agcaccacgt caacggcatt tgcccggcac cacttaacgg cgtcttcacc
    15601 gcacgatgcc tcaccgacga cttttatacc ctttatatct tccagaatgc gtcgtatccc
    15661 tgcgcgcacc agttcgtggt catcaacaag tagaacgttg atcaaaggaa tatctccaga
    15721 aatagggata acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt
    15781 tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt ttttgtaaat tcgattgtcc
    15841 acagttatgg tcacgcccgt caccaacggc ttcagggatc gtttcacagt aagccctgcg
    15901 atgaaatgtg acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg
    15961 taacattaat taacccgaac aatacgggca aaaaacattc atgataaaaa tatttattgg
    16021 tcattatatt aacgtatttt atagtactgt tgatatcacg ctcaaaaaac aaccactgct
    16081 atttttagca aagcttatgg tatactccgc cgccttaaca tttttcaccg caaattttca
    16141 ttgcaacatg acgaggaaaa taaatgagta cgcctgattt ttctactgcc gagaataatc
    16201 aagaactggc aaatgaagtc tcctgcctga aagcgatgct gacgctgatg ctgcaggcga
    16261 tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg
    16321 aagacgaaac ccaggctgca gtattttcca aaacggttaa gcaaattaaa caagcctacc
    16381 gtcagtaata taaaaccggc tgatagcgtg cctttcagcc ggtttttgca tctggcacgc
    16441 aggacagaaa agagatcaaa ttaagccagt agcggccgcg taacaggcaa cctgggtctt
    16501 atttggtgca ttaatttttt tctgcatgtt tttctggtgg aaattgaccg tattctccga
    16561 gattgacaaa atcatcgcta tctctgctga tgtcttccct tccgccgtcc acctcagaat
    16621 ttctttttcg cgcttgctga aattcatctc tggcgtcatc actatttcat catttaaacg
    16681 catcagagcc atcagacttt cgcgcaccag taactgcatt tttaattgca gttcatcact
    16741 aagaatgggt atttcgcgcg cgctgcaacg ggaaaaggac aaaaagccca gcgcccggtt
    16801 tggcagcatt aaatactgag tgacaccgcg gcgtaaacca tgtgcgcgcg cggcttccca
    16861 taacggctgt gcttcgctga ataagtcatc attccacatt aaatggccct gactaaagtt
    16921 ttcagggttc agcaccggat caattgcgag aaagtttttt gcctgataat aactaaccca
    16981 cgcctcaggg taattggtgt aaaaagccac tttaggtcgc gtgaatggta ccgggtggcg
    17041 aacacataac gaatagtaat cgtactccag cagctgagcc tgaagctcta tttcatggta
    17101 gacctcttct gcggcctcca tcctctgaaa acgcaacagc atcgtgcgac gccagctgaa
    17161 aaaatcctta tcctgcatag taaaccgcaa cgcccctgag agtgagtatc atttataatg
    17221 ataattcaaa ctatcacata aataagattt atatataatt tatattattc agacaatgaa
    17281 ttacttttgc aagccatcgc attctcttat gttattaatg agttatgctg atttgttaaa
    17341 cagttttatc acgcttgaaa tggcgttccg ccccgactgt cgaatcgtcg gggctgattt
    17401 tttcttatta ttgcagcaga aacttctcga ggaactggcg ggtgcgaggc tgctcgggat
    17461 cggcaaataa cgcttttgcg gccccctgct cgactatccg cccctggtcc ataaagatcg
    17521 cccggtccgc aacatcccgg gcaaagctca tttcgtgcgt cacaatcacc atcgtgcgct
    17581 tttcctgcgc cagctgacgg atggtgttca ggacttcgcc caccagctct ggatccagcg
    17641 ctgacgttgg ctcgtcaaac aaaatcacct caggacgcat tgccagcgca cgcgcaatcg
    17701 caacacgctg ctgttgaccg ccagacaaac gacgtggata gctggtttct ttaccggcca
    17761 gcccaacttt cgccagtagc tcgcgagcgc gcgccgtggc ctcttctttc gattcgcctt
    17821 tgacgatcac cggcccttca ataatgttct ccagcaccgt acgatgcgga aacaaattaa
    17881 agttctggaa gacaaacccg acgtgctgac gcaactggcg aatcagcgat ttttgctgac
    17941 ttaatgaacg tgcagtatca atagtgatat cgccaacggt gatagttccc gcttccggtt
    18001 gttccagcag atttatgctg cgtagcaacg tggttttgcc ggaaccactc ggaccgataa
    18061 ttgccaccac ttcgccaggc tttacctcaa ggtcgatacc gtgcagcacc gtctgaccgt
    18121 gaaatttttt caccaggttc ttaacttcaa tggcactcat tttggttctc tctcctggcg
    18181 attaagttga ttctcaaaat ggttctgcaa cgtcgataac acagtcgcca tgatccagta
    18241 gatgagcgaa gccgccaggt acatggtgaa cacctccagc gtgcgcgagg taatcaactg
    18301 cgcttgacgg aacagctccg gcacctggat tgtcgcggcc agcgacgtat cttttaccag
    18361 gctgataaag ctgttcgaca gcggcggcaa cgccacccgc gccgcctgtg gcaaaatcgc
    18421 gcgacgcata gtctgccatg gcgtcatacc aatactggca gctgcttccc actgaccttt
    18481 atcaatagaa gaaatggcgg cgcgaagcgt ttcggcagca taggcggcag tgttgagcga
    18541 cagaccaatc attgctgacg gaatgggatc taattcgata ccaaactgcg gcaggccgta
    18601 atagatcata aacagttgtg cgatgagcgg cgtaccacga aaaatagaga tataaaaacg
    18661 cgccagccag cgcacgggcc agattggcga caggcgcatc agcgcgagaa taaacccgag
    18721 cagtaagcca aaaaacatgc cgccgatact gagttgcagc gtatacccag cccctttcag
    18781 caggaacggc aaggaatcaa taaccagttg tatactttct tgcatgagcg tttccggagc
    18841 taaacgtggg gatgataggc gaacagcgca ggcgcgccgc cggtatgaat aaacagaatc
    18901 ggcccttcat ctttgaagcg tttctgactg ataccgtcaa tcagacccgc catcgctttt
    18961 ccggtataca caggatcaag cagaatgcct tccagccgcg ccagcaattt cactgcttcc
    19021 atgccttcgt cgttcggcac gccgtagcca ggtgcaaaat agtcatccca gagtaaaatt
    19081 tctgctgatg cggtcagctc cagttctttc gcaatcgcct gttgtaggtt aaccactttc
    19141 ggcaattgat cggcaacgga acgcgacacg gtcacgccaa tcagttcgct ttcaggcatc
    19201 aggtgttcca gcccaacagc cagtccggcg tgagttccgg cactgcccga tgcgactacc
    19261 accgacgaaa tattaaccgc cccttcacac tgttgcgcga tttccagcgc actctccaca
    19321 taacctagcg cgccaagaac attagaaccg ccaaccggaa tgacatatgg gcgaaagcct
    19381 tgtgcttcga ctcgcgtcgc cagctcttcc agttgggcat tgggatcggt cagtgcgtcg
    19441 cacatttcaa tctgggtatt gaacagatcc agcaacaagc gattgccgtt ggttaaatag
    19501 ttttctgcgg ttgtgccaat aggattttcc agcagcgcca cgcagtgcag accgagtttg
    19561 gcagcgactg cggcagtctg gcgcacatgg ttagactgga tcgccccggc agtaatcagc
    19621 gtatcggcac cttcacgcag agcatctgcc gcgagaaatt ctagcttacg taatttattg
    19681 ccgcccattg ccatgggggt gacgtcatcc cgtttgatga aaatttcccg tccaagataa
    19741 tcagaaaagc gcggcagata ttcgagcggc gttggcgcgc cgataaactc cagccgtgga
    19801 aaacgggtta aattatgcag tggcataaca gcctccgatg tgtgttgttg tgattttctt
    19861 attatgcacg ctgaaaacgc gtaaataaaa aaggcgctag tgaaagcgcc cttttttgtc
    19921 attatgctga ttatttggtc acatcagcac caaaccattt ttcggaaaga gcttgcagag
    19981 tgccgtcttt ttgcatttcc gcaattgcat cattcactgc tttcagcagg tcttcatttc
    20041 ctttacgcag cgccacgcca gactcctgac gggagaatgc ttcaccggtt actgccagcg
    20101 tatcgttggt tttcttcacc agatccagcg ccgccagacg atcaacgagg atcgcatcga
    20161 tacgccctac gcgcagatcc tgatatttgg tcgggtcatc atcataggta cgcacatcga
    20221 cgccctgaac attctgccgc agccactctt catagttggt gcccagaccg acacccactt
    20281 ttttgccttt cagatcagca gccgttttaa tggtgccttc gttacctttt ttcaccagcg
    20341 cctgaatacc agaaatggtg tacggggttg agaaatcgta ttttttcttg cgctcatcag
    20401 aaatggtgac ctgattaatc accacatcaa tacgtttaga gtccagcgac gccagcatac
    20461 cgtcccattt ggtcggtttt agggacgcct caacgccaag atgttttgcc agctgttggg
    20521 caaattccac ttcaaaaccg gttaatttgc cgtcatcccc ctgaaaactg aacggcggat
    20581 aagttccttc cagacctacc agcagcgtgc cgcgctcttt aactttatta agcagacctt
    20641 catctgcaaa acttttaacg ctcatgcccg caaccagcgc cacggccatc acacccatca
    20701 atgcctgacg tcccagatgt gctaatttca tattcacccc gaatgttgtt atgtctgttt
    20761 gcagtgtaga gccattgttt gtaaacacaa aaacaactcc gctacatctt attcttattt
    20821 aatatatatc agaagaaggc aggctggagg atttctgcac aagtccggta caggtttggc
    20881 gctgatagtg ttgatacttc cgtaacgcga tgcggtaatt gttggtgctg gtggtgggca
    20941 gccacggggc gaggatttca tcaaggaaac cgtcctgcag ctgatcgagg gaaatatttt
    21001 gctcgtgtag atgattcccc aaacggcgca gacgaacgac atattcacgc accgtaccag
    21061 ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg aaaaaatcac
    21121 tctgcgtttt gcaatgtaaa tcaccgcaaa atcggcacaa tgccgcccat gatttttgtt
    21181 ccgtttgcca gccattttca tcaagcagcg tgtccaggcg ggaaatctcg attttattca
    21241 ctattttgcc gtcgcgaact aaggtaatgc gatcgagtaa tttacggcaa tgcgcgcaat
    21301 gggtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt ttcaggtgct
    21361 gcaccatcat taagaactcc tggtagtcaa agttaaagtg cggcatttac tgacgttata
    21421 acttacccag tttagtgcgt aaccgtttaa tagcctggct gtgtaactga ctgacccgcg
    21481 attccccgac ctccagcact gcaccaatct ctttgagatt cagctcttcc tggtaataga
    21541 gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc acccgctggc
    21601 gcagattatt gtccagcagt tgttgtagcg ggttttctcg ctgatgatca tcagtaacca
    21661 gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc tggctgttat
    21721 tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt tccgctacct
    21781 cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc tgtgccactt
    21841 cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt tcatccagca
    21901 tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt agggcgtcat
    21961 agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg tcaagttcca
    22021 cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc gggacataac
    22081 gctgccatag cgagtgttta tccattacac cttcagcggt atagagtgaa ttcacgataa
    22141 acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggg tttttaatcg
    22201 gacgattagt gggtgaaatg aggggttatt tgggggttac cggtaaattg cggacagaaa
    22261 aacccccgcc ggtggcgggg gagcacgttg ctgacaaatt gcgcttgatg ttgccggatg
    22321 cggcgtaaac gccttatccg gcctacaaaa atgtgcaaat tcaataaatt gcaattcacc
    22381 gtgtaggcct gataagcgca gcgcatcagg caatttggcg ttgccgtcag tctcagttaa
    22441 tcaggttagg acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct
    22501 tttgccagca cggagttacc ggcctgctgg atgatctgcg ctttcgacat attggacact
    22561 tcggtcgcat agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg
    22621 ttgttcaggt tggtgactgc ggaatccaga cggttctgca ccgcaccgag ggaagaacgg
    22681 aatttgtcta cagatgcgat agcatcgtcc agcgctttca gcggatccgt ggtcgcttcg
    22741 gatttaccag tagcttcagt ggtcagagaa ccgtcagccg ctttgtacag agccgcgccg
    22801 tcatcatcca gaatattgcc gttagcaaat tcgtgataag tttctgtgac agtcttgttg
    22861 gtcgcagtcg tcagttcacc atcagcacca atatttacgg tagcgccacc tgtagtagcg
    22921 gttactgccc caccagcggc aacgtcaaaa gactgtgcgc cgttagctac ggtataagcc
    22981 tgcccagtta acgcagtgtc catatcttcg gcggataccg ttgcgccctt aatatccaca
    23041 tttttgctag cctgggtgaa atcaacggtc atgccgctgc cgaatgtaat cttcgcatca
    23101 actgctgcat taccattcgc accgttgaaa ataccgtcca acgttgccgc atctgggccg
    23161 ccggcctggt ttttagtcag gttgccgtca gaaccaatat ataaagcatc accattgacg
    23221 tcagtcaggg tgccatcgct ggcaatttta acgtcctgag cgcttccgcc gatggagatc
    23281 tgagccgtat tatcaccgtt tgctgcatag gctttcagca catcagcaac tttagtggtg
    23341 gccccagtac catcgccatt ggtaagtgcg gttgcgtcaa atgaataaga cttgctggtt
    23401 gcgttgtagg tatatgcagt agaagccgct acgcctaatc cattgtttgt accggtataa
    23461 ccgctaatag tgctgcctcc gctaacgctt gccagcacgt cggtagctgt tgctgcatta
    23521 aaggtagtaa cagtagattt agtccaggtg ctgttgccac tgacgactgc accttgggag
    23581 aagcccgcag cggtcagatc tgcttcagtg gctttagcat tatcaacagc gcctttaccg
    23641 ttaacattga agctggtcag cttcaatgta gaagagtcga tctttttcag atcaatcgtg
    23701 atggtttcac catcatttgc gccgacctga attttcatcg agccatcttt agacagcacg
    23761 ttcacgccgt tgaactgggt ctgaccggat acgcggtcaa tttcgtccag acgagatttg
    23821 atttcgtcct ggatggagtc caggtcagaa tcggagttag taccggtagt ggcctgaacc
    23881 gtcagttcac ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcggtggtc
    23941 tgtgcaacag aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatatta
    24001 gaggtgaaac ggttagcaat cgcctgacct gcggcgtcat ccttcgcgct gttaatacgc
    24061 aagccagaag acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta
    24121 ttttgagtga tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct
    24181 atattgcaag tcgttgatta cgtattgggt ttccacccgt cggctcaatc gccgtcaacc
    24241 ctgttatcgt ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt
    24301 tagtcgccga aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa
    24361 cgtaaacttt gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata
    24421 aaaggaaatc gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt
    24481 tcaatcctcg atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag
    24541 caatcgtcgt ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact
    24601 ttccagaccg ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcactacc
    24661 agcagcacca ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc
    24721 atcagcgtca cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat
    24781 acgaaaacgg cgatcgccac cagcgacagt aaactcacca ttcaacaagg cggcgacaaa
    24841 gatccgatta ccattgatat cagcgcggct aactcgtcgt taagcgggat ccgtgatgcc
    24901 atcaacaacg caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat
    24961 cgtctgtcag tcacatcaaa tgacaccggc cttgacaatg cgatgacact ctcggtcagc
    25021 ggtgatgatg cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggcatg
    25081 gaagtctcgg tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc cattgagaac
    25141 agcagcaaca ccatcagcga cgcgctggaa aacatcaccc tgaacctgaa cgatgtcacc
    25201 acgggcaacc aaacgctgac catcactcag gacacctcca aagcgcaaac ggcgattaaa
    25261 gactgggtga atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc
    25321 gccgtagatg cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc
    25381 acgctgcgga cgattcagac gcagttgaaa tcgatgctga gtaataccgt cagttcttcc
    25441 aactataaaa cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa
    25501 ctggatgccg acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg
    25561 attgttggcg atggtaaaaa aaccggcatc acgaccacca tcggcagcaa cctgaccagt
    25621 tggctttcga caacgggcat tattaaagcc gctaccgatg gcgttagtaa gaccctgaat
    25681 aaattaacta aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac
    25741 aaagaacaat ttacccaact ggacgtttta atgacctcgt taaacagcac aagcagctac
    25801 ttaacgcagc agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag
    25861 gtcatgtacg cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcgccgta
    25921 atgagcgcca gccagcagca gctggtcacc atgctatttg atggagtgct gagcgcactg
    25981 gttagagcgc gcctgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca
    26041 aaagcgatca acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac
    26101 gaattaaccc aaaacttgat tgctctttat agctatatgg tcaggcgctt gctgcaagcc
    26161 aatttacgca acgatgtctc cgcagtcgaa gaagtggaag cattaatgcg taatattgcc
    26221 gatgcctgga aagagtcttt actctcccct tctttgattc aggacccagt ctgatgaacc
    26281 atgcaccgca tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac
    26341 gcctggcaac ggaagaacaa tgggacgaac tcatcgccag cgaaatggcg tatgtgaatg
    26401 cggtgcagga gattgcacat ttgactgaag aggttgaccc gtccaccacg atgcaggagc
    26461 agctccgccc gatgctgcgc ctgattctcg acaacgaaag caaggtaaag cagttattac
    26521 agattcgaat ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt
    26581 taagtgccta tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa
    26641 tctgaatgag tcgatggctc gcgaataatc cgattacggc tacgcttcta atgttcccct
    26701 tgaatggagt cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc
    26761 ggaaggcggt aagctctggc ctgaactggc cgagcgcgcc gacggtttta atgatattgg
    26821 tatcaatatg gtctggttgc cgcccgccta taaaggcgca tcgggcgggt attcggtcgg
    26881 ctacgactcc tatgatttat ttgatttagg cgagtttgat cagaaaggca gcatccctac
    26941 taaatatggc gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat
    27001 tgcggtgctg ttggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat
    27061 tcgcgtgcag cgtgtaaatg ctgacgaccg tacgcaaatt gatgaagaaa tcattgagtg
    27121 cgaaggctgg acgcgttaca ccttccccgc ccgtgccggg caatactcac agtttatctg
    27181 ggatttcaaa tgttttagcg gtatcgacca tatcgaaaac cctgatgaag atggcatatt
    27241 taaaattgtt aacgactaca ccggcgaagg ctggaacgat caggttgatg atgaactcgg
    27301 taatttcgat tatctgatgg gtgagaatat cgatttccgc aatcatgccg tgacggaaga
    27361 gattaaatac tgggcgcgct gggtgatgga acaaacgcaa tgcgacggtt ttcgtcttga
    27421 tgcggtcaaa catattccgg cgtggtttta taaagagtgg atcgaacacg tacaggaagt
    27481 tgcgccaaag ccgctattta ttgtggctga atactggtcg catgaagtcg ataaactgca
    27541 aacgtatatt gatcaggtgg aaggcaaaac catgctgttc gatgcaccgc tgcagatgaa
    27601 attccatgaa gcatcgcgca tggggcgcga ctacgacatg acgcagattt tcacgggtac
    27661 attagtggaa gccgatcctt tccacgccgt aaccctcgtt gccaaccacg acacccaacc
    27721 gttgcaagcc ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgctctaat
    27781 tttgttgcgg gaaaatggcg ttccttcggt attctatccg gacctctacg gtgcgcatta
    27841 cgaagatgtc ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct
    27901 tgatgagtta attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga
    27961 ccatccgaac tgcattgcct ttagccgcag tggcactgac gaatatcccg gctgcgtggt
    28021 ggtcatgtcg aacggggatg atggcgaaaa aaccattcat ctgggagaga attacggcaa
    28081 taaaacctgg cgtgattttc tggggaaccg gcaagagagc gtaataaccg acgaaaacgg
    28141 cgaagcaacc ttcttttgca acggcggcag tgtcagcgtg tgggttatcg aagaggtgat
    28201 ttaaattcat ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg
    28261 gcgctttatc cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca
    28321 taccatcgta ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg
    28381 taacgttagt gagatcatca gacatcaatg tcaacttacc cggcacggca atcacccgct
    28441 gccactgacg gcaatccaga gtatcgcccg ctttagtgac aatcaggctg cctatcgctt
    28501 ccggactgac cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg
    28561 gcgcgggggt tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca
    28621 tgagcgcgcc tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga
    28681 ttgtggcatt aaagccctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta
    28741 actattattt ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcgac
    28801 tcttgtttca aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca
    28861 cgcctggttg attaaattct gggcgcccat ccccgcggtc atcgcggcgg gtattctctc
    28921 cacttactat tttggcatca ctggcacctt ttgggctgtc acgggtgaat ttacccgttg
    28981 gggcggtcag ctcctgcagc tgttcggcgt ccatgctgaa gagtggggtt attttaaaat
    29041 tatccatctg gaaggatcgc cattaacccg catcgacggg atgatgatcc tcggtatgtt
    29101 tggcggctgc tttgccgcag cgctgtgggc caacaatgtc aaactacgca tgccgcgtag
    29161 ccgtatccgc attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct
    29221 ggcaatgggc tgtaacctgg cggcgttctt taccggtatt cctcagttct cgctgcatgc
    29281 ctggttcttt gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct
    29341 gcccatcttc cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca
    29401 aaaaccggat caggcgcgac gtcgttttcg tctcgggatg ctggtctttt tcggcatgct
    29461 gggctgggcg ctgctcacgg cgatgaacca acccaaactg gggctggcaa tgctgtttgg
    29521 cgtcggcttt ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga
    29581 tatgtggatc accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag
    29641 tgccatcggg atcttcagtt acgtacagtt aggcgttgaa cccaaaatca tgtgggcggg
    29701 accaaacgcg gtaattggtg gtttactgtt tggttttggc atcgtgctgg ctggcggctg
    29761 cgaaaccggc tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct
    29821 gggcaatgtg atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct
    29881 ggccaccgac tgggacaaaa tcaacctgct gaaaaccttt ggcccgatgg gtggcctgct
    29941 ggtgacatat ttgctgttgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt
    30001 cttccgccgt gcggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat
    30061 taccgtcttg atatggtggg tgaaccctgc ccttatccgg cagtcgcaac tcttgaggcg
    30121 atgccgcagt taaaaaaagg ggaaatcctg gaagtggtga gcgactgtcc gcagtcgatc
    30181 aataatattc cactggatgc gcgtaatcac ggctatacgg tgctggatat tcagcaagac
    30241 gggccgacca ttcgttattt aattcagaag taatccctca ttcccgctgt gacccacagc
    30301 gggaatatcg ttgcctctgt accacataga ttttacccgt cccctctcct attcttttca
    30361 gtgtgctctc aggaggtttc tatgtgtggt cgctttgccc aatcccaaac gcgtgaagat
    30421 taccttgcgc ttctcgctga agatattgaa cgcgatattc cctatgatcc cgaacccatt
    30481 ggcagataca acgtcgcgcc gggaaccaaa gtccttctgc tcagtgagcg tgatgaacac
    30541 cttcatctgg atccggtttt ctggggatat gctcccggat ggtgggataa accgccgctg
    30601 attaacgccc gcgtagaaac tgcggcaacc agtcgtatgt ttaaaccgct ctggcaacat
    30661 ggtcgggcaa tctgttttgc cgatggctgg tttgagtgga aaaaagaagg cgacaaaaag
    30721 cagccttatt ttatctatcg tgctgatgga caacctgttt ttatggcagc gataggcagc
    30781 acaccgttcg agcgcggtga cgaagccgaa ggatttttga ttgtcactgc agcagcagac
    30841 cagggtctgg tagatattca tgaccgccgc ccgctggttc tgtcgccaga agctgcgcgg
    30901 gaatggatgc ggcaagagat tagcggtaaa gaagcctcag aaatcgcggc cagtggctgt
    30961 gttccagcaa accagttctc ctggcacccg gtatcgcgcg cggtgggtaa tgttaaaaac
    31021 cagggtgcgg agttaattca acctgtttga taagcctgga gattattgat caacaatact
    31081 gcgtcataag taatctctat tagacaaaga tttcattacc tgttggcata ttgcaaaaat
    31141 aacaccaata cggaatcgtc atgttcacga ttaaaacaga tgatctcacc catccagcag
    31201 tgcaaacatt agtggcttac catatttccg acatgctgca gcagtctccc cccgaaagca
    31261 gtcatgcttt agatgtgcaa aaattacgta acccgacagt gacattctgg tcagtatggg
    31321 aaggcgaaca actcgcagga atgggtgcgc tgaagttgct ggatgataaa catggcgagc
    31381 taaaatcaat gcgtaccgcg ccaaattatt tacgtcgtgg tgtcgccagt ctgattttac
    31441 gccacatttt gcaggtcgcc caggacagat gccttcatcg cctgagctta gaaacgggta
    31501 cacaggctgg atttacggcc tgccatcata tttatttgaa gcatggtttc gttgattgcg
    31561 aaccgtttgc tgattatcaa cttgatccac acagtcgatt tttatcattg acgctatgcg
    31621 aaaataatga gttgctatga gccagacgca gcacattctt gcattcgacg tgctgcgcct
    31681 ttatttatca ccagcaggaa acgccttgtc catagacgcc acttctacat gcgccacaag
    31741 aaacctctat tccagtgaca caattgcgcc taattaatta catataatat ttaattatga
    31801 gctcctcacc atctattaca tactttttaa ccatatcgga atatttatca taatcatcgg
    31861 ggttcgtaac aatatatttc cgctgcgata tttcataggt aatgtctgtc aaagtccatg
    31921 gcattaaaaa tgcctcttta ataggattac attccatgaa aagtaatttt aaattgccag
    31981 gtatcgcagg aataacctca atcttattat attcaatata cgcttctttc agatttttgg
    32041 ggaaccatct aatttcttta atattattct cactacaatc aaaaacctta gcggtgtctg
    32101 gtagtgctgg tatttccgat agcttattgt gacaaataga aattatctta gcctttggcg
    32161 gaaatgagtc aatatgcgat atcaggttat tatttacgga gatcagggta attccatcag
    32221 gtacttgtgg aaattctgta aggttattga aatcgacttc taattctttg catgattcgg
    32281 gcacctgtat tgaatcaagt tcattataaa caaggctaag catttcaatc cactcaggaa
    32341 gaggcggcac agaacccaaa aataggtatg ataagtcaag ttcattactg tgaagcttaa
    32401 cacatttaaa cattctcacc aatgctctat ccacattttc tttgaagtca ttcgatgcgc
    32461 cttctttgac tgaactagcc cagtcactga aacctttgca aagtctggca tctgtaattt
    32521 tggttaactt gtccagcaac tctgcaatat tggtcgatat aaactctaat ttatgccgaa
    32581 tgggtaaagt cccctccccc tgactttttt ctatcgtgat agatcccgtc ttatcataag
    32641 cc
//
LOCUS       DAZGBC010000038        32197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGBC010000038 DAZGBC010000000
VERSION     DAZGBC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.038"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..1904)
                     /gene="ycaO"
                     /locus_tag="ACHK1J_001816"
     CDS             complement(144..1904)
                     /gene="ycaO"
                     /locus_tag="ACHK1J_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO7911093.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSNNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRDTILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            2034..2726
                     /locus_tag="ACHK1J_001817"
     CDS             2034..2726
                     /locus_tag="ACHK1J_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO7911094.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2925..4013
                     /gene="serC"
                     /locus_tag="ACHK1J_001818"
     CDS             2925..4013
                     /gene="serC"
                     /locus_tag="ACHK1J_001818"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO7911095.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4084..5367
                     /gene="aroA"
                     /locus_tag="ACHK1J_001819"
     CDS             4084..5367
                     /gene="aroA"
                     /locus_tag="ACHK1J_001819"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO7911096.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKIIYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5536..6300
                     /gene="ycaL"
                     /locus_tag="ACHK1J_001820"
     CDS             5536..6300
                     /gene="ycaL"
                     /locus_tag="ACHK1J_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO7911097.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6473..7156
                     /gene="cmk"
                     /locus_tag="ACHK1J_001821"
     CDS             6473..7156
                     /gene="cmk"
                     /locus_tag="ACHK1J_001821"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO7911098.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7267..8940
                     /gene="rpsA"
                     /locus_tag="ACHK1J_001822"
     CDS             7267..8940
                     /gene="rpsA"
                     /locus_tag="ACHK1J_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO7911099.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9100..9384
                     /gene="ihfB"
                     /locus_tag="ACHK1J_001823"
     CDS             9100..9384
                     /gene="ihfB"
                     /locus_tag="ACHK1J_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO7911100.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9591..11855
                     /locus_tag="ACHK1J_001824"
     CDS             9591..11855
                     /locus_tag="ACHK1J_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO7911101.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQLTVNFSAQGWLISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11892..13640
                     /gene="msbA"
                     /locus_tag="ACHK1J_001825"
     CDS             11892..13640
                     /gene="msbA"
                     /locus_tag="ACHK1J_001825"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO7911102.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13637..14623
                     /gene="lpxK"
                     /locus_tag="ACHK1J_001826"
     CDS             13637..14623
                     /gene="lpxK"
                     /locus_tag="ACHK1J_001826"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO7911103.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVVDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14660..15892
                     /gene="ycaQ"
                     /locus_tag="ACHK1J_001827"
     CDS             14660..15892
                     /gene="ycaQ"
                     /locus_tag="ACHK1J_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO7911104.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15944..16126
                     /gene="ycaR"
                     /locus_tag="ACHK1J_001828"
     CDS             15944..16126
                     /gene="ycaR"
                     /locus_tag="ACHK1J_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO7911105.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16123..16869
                     /gene="kdsB"
                     /locus_tag="ACHK1J_001829"
     CDS             16123..16869
                     /gene="kdsB"
                     /locus_tag="ACHK1J_001829"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO7911106.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLKRVRAEMR"
     gene            17023..17916
                     /locus_tag="ACHK1J_001830"
     CDS             17023..17916
                     /locus_tag="ACHK1J_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO7911107.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17893..18672)
                     /gene="elyC"
                     /locus_tag="ACHK1J_001831"
     CDS             complement(17893..18672)
                     /gene="elyC"
                     /locus_tag="ACHK1J_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO7911108.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLSLLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRDNPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18808..19593
                     /gene="cmoM"
                     /locus_tag="ACHK1J_001832"
     CDS             18808..19593
                     /gene="cmoM"
                     /locus_tag="ACHK1J_001832"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO7911109.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19590..20912
                     /gene="mukF"
                     /locus_tag="ACHK1J_001833"
     CDS             19590..20912
                     /gene="mukF"
                     /locus_tag="ACHK1J_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO7911110.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20893..21597
                     /gene="mukE"
                     /locus_tag="ACHK1J_001834"
     CDS             20893..21597
                     /gene="mukE"
                     /locus_tag="ACHK1J_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO7911111.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21597..26057
                     /gene="mukB"
                     /locus_tag="ACHK1J_001835"
     CDS             21597..26057
                     /gene="mukB"
                     /locus_tag="ACHK1J_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO7911112.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVEQ
                     RHLADQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNNDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26318..28165
                     /gene="ldtD"
                     /locus_tag="ACHK1J_001836"
     CDS             26318..28165
                     /gene="ldtD"
                     /locus_tag="ACHK1J_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7911113.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28346..28894
                     /locus_tag="ACHK1J_001837"
     CDS             28346..28894
                     /locus_tag="ACHK1J_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO7911114.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28921..29568
                     /gene="gloC"
                     /locus_tag="ACHK1J_001838"
     CDS             28921..29568
                     /gene="gloC"
                     /locus_tag="ACHK1J_001838"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO7911115.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLKEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29619..30809)
                     /gene="aspC"
                     /locus_tag="ACHK1J_001839"
     CDS             complement(29619..30809)
                     /gene="aspC"
                     /locus_tag="ACHK1J_001839"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO7911116.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30994..32067)
                     /gene="ompF"
                     /locus_tag="ACHK1J_001840"
     CDS             complement(30994..32067)
                     /gene="ompF"
                     /locus_tag="ACHK1J_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO7911117.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7826 a         7858 c         8662 g         7851 t
ORIGIN      
        1 attaaataca aattaccgat atttaacctt ataattacaa ttattttatt aatgcaaata
       61 tatgtaaagc ggggcattaa aaaaacgccc cgtaatataa ctcagacgaa tcattaagcc
      121 tacattgcgt aggctatttg attttatttt gcccagaatg ctgctttggc gcgctgcagc
      181 ttttcgtagg ccttcaacag cgactgatgt gcagcaaacg cgtgcagatc gctatctact
      241 ggttgcaagc cataaaacgc cgcttcgccg ctcattgccg cactagcggc ttccacggca
      301 tctgcgccgt acatgcgaac aaacgcattc agatattgca gcggctggcg atcttcttcc
      361 tgcgccagta ataacaacgt ttgcaggcag cgataatagt tggcgcgttc cgggctgaat
      421 accgatgagt taaactccat cgtccattcg gtccagacca gagcctgttc cagatcgcca
      481 ccagccagcg ccagcatggc ttttaattca ccgatacgca gggtgtacca accgttatcc
      541 gacccggtcg ccagacccaa cagctcacgc acgcgggtaa agtcatcaaa accttcttca
      601 tccagttgct cgatgaggtt caggtaatct tctttttccc actcgctgcc tggtagcgag
      661 aggatggtgt cgcgcagatg actacccata ctgttattcg ctaaccacaa atcttcagcc
      721 ggataaatat cggacatgcc aggcacgata atacggcaag cataaacgcc cagatgctcg
      781 taatcggcaa tataaacttc tttatcttct ttgttgaaga tagccatcag cgtggcaaac
      841 tcttcttccg tggtgccgga gaaattccag tccacaaacg gataatcggc atcctgcttg
      901 aacaggtccc aggagattaa accgctggaa tcgataaagt gcgtttcgag gttggtatgt
      961 tcagcgactt cttcatcatc gaaggttggc ggagtaaaca catccaaatc tttcaggcca
     1021 cgaccttgca gcagctcggt cacggtacgt tccagtgcta cgccaaaatc aggatgcgca
     1081 ccgaaagagg caaagcaggt gccgttagca ggattaaaca gtaccacgca aatcaccgga
     1141 tactggccgc caagcgaacc gtcataagcg aagatcggga aaccttccgc ttccagcgtt
     1201 tcgatcgctt caactactgc tggataacgt gccagcacgt ctgccgggat ttccggcaag
     1261 ctgatgcttt cagcaataat gcggtttttc acgtagcgtt cgaaaacttc ggacaacccc
     1321 tgaacgcgtg cttcgttacg ggtattgcca gcagacatac cgttagaaac gtacaagtta
     1381 ccaatgatat tcatcggaat ataaacggtc tgattattag actgacgtgt aaacggcaga
     1441 ccgcaaatac cacgatcttc gttaccggat tgtaggtcaa tcagcatact gccggtcaat
     1501 tcattctccg gatcgtaaaa tgcgcgcaga cggtcatcga gcagcccttc tggcacatcg
     1561 tcattttcgg tcagtgggaa ccatttttcg ttgggataat gcacaaatgg accgttggcg
     1621 atggtttcgc ccagccagaa atcagcaaaa aagtagttgg ttgagagacg ctcgaaatat
     1681 tcaccgagtg cagaagccag cgccgctttc ttggttgcgc ctttaccgtt ggtaaaacac
     1741 agtgcgcact ctttgtcgcg aatatgtaca gaccagacgt taggcacggg attcagccag
     1801 gaggcctctt caatctgaaa gccgaggtct gaaagttttt gctggaagcg agcgatggaa
     1861 tcttccagtg cggcatcttt gccggggata aatgtttgcg tcatgaaaat cactttagtc
     1921 gtacggaaag cgcgcaataa tacgggtttt atctcaaagg cgctatcacc gccgccatgc
     1981 cgatgaactg ttgactatgc ttttagcgga taaaccacgt taagagcata aaaatgaagg
     2041 cgttcgatct ccaccgtatg gcatttgata aagtgccttt tgatttcctt ggcgaagttg
     2101 cactacgtag tctgtatacc tttgtactgg tttttttgtt cctcaaaatg accggaagac
     2161 gcggagtgcg gcagatgtcg ctgtttgaag tgttaatcat tctgacgctg gggtcagcgg
     2221 cgggagatgt ggcgttttat gatgatgtgc cgatggtccc ggtacttatc gtctttatca
     2281 ctctggcgtt gttataccgc ctggtaatgt ggttgatggc acacagtgaa aaactggaag
     2341 atcttctgga aggtaagccg gttgtcatta ttgaagatgg cgagctggcc tggtcgaaac
     2401 tcaataactc caacatgacg gaatttgagt tctttatgga gctacgattg cgcggtgtag
     2461 agcagttggg gcaagtacgt ctggcgattc tcgaaaccaa cgggcaaatc agtgtctatt
     2521 tctttgaaga tgacaaggtg aaaccgggtt tacttatttt acccagtgat tgtacgcagc
     2581 gttacaaagt ggtgccggag tcggcggact atgcctgcat ccgttgtagt gaaatcattc
     2641 atatgaacgc gggggaaaaa caattatgtc cgcgctgtgc aaatccagaa tggacgaagg
     2701 caagtcgggc taaacgggtg acctgacagc aaaaatatcg gttttttgct aataatccga
     2761 gagattctgt tgtgtgacgc gtgccgcatt tttgccctca acggttttac ccattgcgat
     2821 gcgtgtcact gaatgataaa accgatagcc acaggaataa catattaccc gcggtcgcaa
     2881 tcgattgact gggggttaat ggcaacgcaa cgtggtgagg ggaaatggct caaatcttca
     2941 attttagttc tggtccggca atgctaccgg cagaggtgct taaacaggct caacaggaac
     3001 tgcgtgactg gaacggtctt ggtacgtcgg tgatggaagt gagtcaccgt ggcaaagagt
     3061 tcattcaggt tgcagaggaa gccgagaagg attttcgcga tcttcttaat gtcccctcca
     3121 actacaaggt attgttctgc catggcggcg gtcgcggtca gtttgctgct gttccgctga
     3181 atattctcgg tgataaaacc accgcagatt atgttgatgc cggttactgg gcggcaagtg
     3241 ccattaaaga agcgaaaaaa tactgcacgc ctaatgtctt tgacgccaaa gtgactgttg
     3301 atggtctgcg cgcggttaag ccaatgcgtg aatggcaact ctctgataat gctgcttata
     3361 tgcattactg cccgaatgaa accatcgacg gtatcgccat cgacgaaacg ccagacttcg
     3421 gcaaagatgt ggtggtcgcc gccgacttct cttcaaccat tctttcccgt ccgattgacg
     3481 ttagccgtta tggcgtgatt tacgctggcg cgcagaaaaa tatcggcccg gcaggcctga
     3541 caatcgtcat cgttcgtgaa gatttgctgg gcaaagcgaa tatcgcgtgt ccgtcgattc
     3601 tggattattc catcctcaac gataacggct ccatgtttaa cacgccgccg acatttgcct
     3661 ggtatctgtc tggtctggtc tttaaatggc tgaaagcgaa cggcggtgta gctgaaatgg
     3721 ataaaatcaa tcagcaaaaa gcagaactgc tatatggggt aattgataac agcgatttct
     3781 accgcaatga cgtggcgaaa gctaaccgtt cgcggatgaa cgtgccgttc cagttggcgg
     3841 acagtgcgct tgacaaattg ttccttgaag agtcttttgc tgcaggcctt catgcactga
     3901 aaggtcaccg tgtggtcggc ggaatgcgcg cgtctattta taacgccatg ccgctggaag
     3961 gcgttaaagc gctgacagac ttcatggttg agttcgaacg ccgtcacggc taatgccgaa
     4021 attttgctta atccccacag ccagcctgtg gggtttttat ttctgttgta gagagttgag
     4081 ttcatggaat ccctgacgtt acaacccatc gctcgtgtcg atggcactat taatctgccc
     4141 ggttccaaga gcgtttctaa ccgcgcttta ttgctggcgg cattagcaca cggcaaaaca
     4201 gtattaacca atctgctgga tagcgatgac gtgcgccata tgctgaatgc attaacagcg
     4261 ttaggggtaa gctatacgct ttcagccgat cgtacacgtt gcgaaattat cggtaacggc
     4321 ggtccattac acgcagaagg tgccctggag ttgttcctcg gtaacgccgg aacggcaatg
     4381 cgtccgctgg cggcagctct ttgtctgggt agcaatgata ttgtgctgac cggtgagccg
     4441 cgtatgaaag agcgcccaat tggtcatctg gtggatgcgc tgcgcctggg cggggcgaag
     4501 atcatttacc tggaacaaga aaattatccg ccgttgcgtt tacagggcgg ctttactggc
     4561 ggcaacgttg acgttgatgg ctccgtttcc agccaattcc tcaccgcact gttaatgact
     4621 gcgcctcttg cgccggaaga tacggtgatt cgtattaaag gcgatctggt ttctaaacct
     4681 tatatcgaca tcacactcaa tctgatgaag acgtttggtg ttgaaattga aaatcagcac
     4741 tatcaacaat ttgtcgtaaa aggcgggcag tcttatcagt ctccgggtac ttatttggtc
     4801 gaaggcgatg catcttcggc ttcttacttt ctggcagcag cagcaatcaa aggcggcact
     4861 gtaaaagtga ccggtattgg acgtaacagt atgcagggtg atattcgctt tgctgatgtg
     4921 ctggaaaaaa tgggcgcgac catttgctgg ggcgatgatt atatttcctg cacgcgtggt
     4981 gaactgaacg ctattgatat ggatatgaac catattcctg atgcggcgat gaccattgcc
     5041 acggcggcgt tatttgcaaa aggcaccacc acgctgcgca atatctataa ctggcgtgtt
     5101 aaagaaaccg atcgcctgtt tgcgatggca acagaactgc gtaaagtcgg tgcggaagta
     5161 gaagaggggc acgattacat tcgtatcact ccaccggaaa aactgaactt tgccgagatc
     5221 gcgacataca atgatcaccg gatggcgatg tgtttctcgc tggtggcgtt gtcagataca
     5281 ccagtgacga ttcttgatcc caaatgtacg gccaaaacat ttccggatta tttcgagcag
     5341 ctggcgcgga ttagccaggc agcctgaatg aataacgtcc aataaatagc caaatctttc
     5401 tttatcaaaa cgccggcaca ttgccggcgt tttttttcgg accttgtgag tcattttgat
     5461 taatggtagc gtcgcttgtc aatgtaagtt gttgatacat aatatttata tatgattaat
     5521 caacggatga ttcacatgaa gaatactaaa ttactgctgg cgattgcgac ctctgcagca
     5581 ttactgacag ggtgtcaaaa tacccacggt attgatacca atatggctat cagctccggt
     5641 ttaaatgcct ataaagcagc aacgttaagc gatgccgatg caaaagcgat tgccaatcag
     5701 ggctgtgccg aaatggacag tggcaatcaa gtcgcaagta aatccagcaa gtacggtaaa
     5761 cgtctggcaa aaatcgccaa agcattgggt aacaatatta atggcacgcc ggtcaactat
     5821 aaggtttata tgaccagcga cgtcaacgca tgggcgatgg cgaacggctg tgttcgtgtc
     5881 tacagtggcc tgatggacat gatgaatgac aacgaaattg aaggtgttct gggccatgaa
     5941 ctgggccacg tcgcgttggg tcactcgctg gctgaaatga aagcttctta tgcgatcgtt
     6001 gccgcacgcg atgccatttc agctaccagc ggtgtggctt cccagctttc ccgctcacaa
     6061 ttaggtgata tcgcagaagg cgctatcaat gcgaaatact cccgtgataa agagtccgaa
     6121 gcagatgatt tctcctttga tctgttgaag aaacgtggca tcagcaccca ggggctggtt
     6181 ggcagctttg aaaaactggc tagcctggat ggcggtcgca cccagtccat gtttgactct
     6241 cacccaccat caacagagcg tgcgcaacac atccgtgatc gtatcgcctc tggtaagtaa
     6301 atcattgtca tctttcgggc tggtcttctg ccagcccgct ataattgcgc aataaatccc
     6361 catctgaata cagacaaaac tggtttttgc acacaacgtt aacgatttgt ggcgtcggcg
     6421 cgtataatgc gcgcggttat gttaacggta cgcctgtttt aaggagataa agatgacggc
     6481 aattgccccg gttattacca ttgatggccc aagcggtgca gggaaaggca ccttgtgtaa
     6541 ggctatggcg gaagcgttgc aatggcatct gctggactcg ggtgcaattt atcgcgtact
     6601 ggcactagcg gcattacatc accatgttga tgttgcttcg gaagatgcgc tggtgccgct
     6661 ggcatcccat ctggatgtac gttttgtgtc gaccaatggc aatctggaag tgatcctcga
     6721 aggggaagat gtcagcggcg aaattcgtac tcaggaagtg gcgaatgctg cttcacaagt
     6781 cgcggcattc ccacgcgttc gtgaagcatt attgcgtcgc caacgcgcgt ttcgcgaatt
     6841 accaggcctg attgccgatg gccgcgacat gggaacggtg gtattccctg atgcgccagt
     6901 gaaaattttc cttgacgcct cctcggaaga acgtgcgcat cgccgcatgc tacagttgca
     6961 ggagaagggc tttagtgtta actttgagcg ccttttggcc gagatcaaag aacgcgacga
     7021 ccgcgatcgt aaccgagcgg tagcgccact ggttccggca gccgatgctt tagtgttgga
     7081 ttccaccacc ttaagcattg agcaagtgat tgaaaaagcg ctacaatacg cgcgccagaa
     7141 attggctctc gcataagcga ccgaatttgc agtacccccg ttgcaatgga atgacagcgg
     7201 gtatgttaaa caaccccatc cggcatggag ccaggtggac gttaaatata aacctgaaga
     7261 ttaaacatga ctgaatcttt tgctcaactc tttgaagagt ccttaaaaga aatcgaaacc
     7321 cgcccgggtt ctatcgttcg tggcgttgtt gttgctatcg acaaagatgt agtactggtt
     7381 gacgctggtc tgaaatctga gtccgccatc ccggctgagc agttcaaaaa cgcccagggc
     7441 gagctggaaa tccaggtagg tgacgaagtt gacgttgctc tggacgcagt agaagacggc
     7501 ttcggtgaaa ctctgctgtc ccgtgagaaa gctaaacgtc acgaagcctg gatcacgctg
     7561 gaaaaagctt acgaagatgc tgaaactgtt accggtgtta tcaacggcaa agttaagggc
     7621 ggcttcactg ttgagctgaa cggtattcgt gcgttcctgc caggttccct ggtagacgtt
     7681 cgtccggtgc gtgacactct gcacctggaa ggcaaagagc ttgaatttaa agtaatcaag
     7741 ctggatcaga agcgcaacaa cgttgttgtt tctcgtcgtg ccgttatcga atccgaaaac
     7801 agcgcagagc gcgatcagct gctggaaaac ctgcaggaag gcatggaagt taaaggtatc
     7861 gttaagaacc tcactgacta cggtgcattc gttgatctgg gcggcgttga cggcctgctg
     7921 cacatcactg acatggcctg gaaacgcgtt aagcatccga gcgaaatcgt caacgtgggc
     7981 gacgaaatca ctgttaaagt gctgaagttc gaccgcgaac gtacccgtgt atccctgggc
     8041 ctgaaacagc tgggcgaaga tccgtgggta gctatcgcta aacgttatcc ggaaggtacc
     8101 aaactgactg gtcgcgtgac caacctgacc gactacggct gcttcgttga aatcgaagaa
     8161 ggcgttgaag gcctggtaca cgtttccgaa atggattgga ccaacaaaaa catccacccg
     8221 tccaaagttg ttaacgttgg cgatgtagtg gaagttatgg ttctggatat cgacgaagaa
     8281 cgtcgtcgta tctccctggg tctgaaacag tgcaaagcta acccgtggca gcagttcgcg
     8341 gaaacccaca ataagggcga ccgtgttgaa ggtaaaatca agtctatcac tgacttcggt
     8401 atcttcatcg gcctggacgg cggcatcgac ggcctggttc acctgtctga catctcctgg
     8461 aacgttgcag gcgaagaagc agttcgtgaa tacaaaaaag gcgacgaaat cgctgcagtt
     8521 gttctgcagg ttgacgcaga acgtgaacgt atctccctgg gcgttaaaca gctcgcagaa
     8581 gatccgttca acaactgggt tgctctgaac aagaaaggcg ctatcgtaac cggtaaagta
     8641 actgcagttg acgctaaagg cgcaaccgta gaactggctg acggcgttga aggttacctg
     8701 cgtgcttctg aagcatcccg tgaccgcgtt gaagacgcta ccctggttct gagcgttggc
     8761 gacgaagttg aagctaaatt caccggcgtt gatcgtaaaa accgtgcaat cagcctgtct
     8821 gttcgtgcga aagacgaagc tgacgagaaa gatgcaatcg caactgttaa caaacaggaa
     8881 gatgcaaact tctccaacaa cgcaatggct gaagctttca aagcagctaa aggcgagtaa
     8941 ttctctgact cttcgggatt tttattccga agattgttga gtttacttga cagattgcag
     9001 gtttcgtcct gtaatcaagc actaagggcg gctacggccg cccttaatca atgcagcaac
     9061 agcagccgct taatttgcct ttaaggaacc ggaggaatca tgaccaagtc agaattgata
     9121 gaaagacttg ccacccagca atcgcacatt cccgccaaga cggttgaaga tgcagtaaaa
     9181 gagatgctgg agcatatggc ctcgactctt gcgcagggcg agcgtattga aatccgcggt
     9241 ttcggcagtt tctctttgca ctaccgcgca ccacgtaccg gacgtaatcc gaagactggc
     9301 gataaagtag aactggaagg aaaatacgtt cctcacttta aaccaggtaa agaactgcgc
     9361 gatcgcgcca atatttacgg ttaagttttt actcaaactt gaacgcgaga aaagcacctg
     9421 tcgggtgctt ttttcatttc tctaatcagg aactggaagc tgcctcgcag agttttgagc
     9481 agttttcacc ctttcgttaa attcttctga atgtgcctcg gggaacgcaa aattcccacc
     9541 agacaaccgc tcaacaaagt tgcacacttt ccataaacag ggaggggtgc atgaaaataa
     9601 cgacagtcgg cgtatgcata atttgcggaa tttttccgtt gctgattttg ccccaattgc
     9661 ctgggacatt aacccttgcg tttctgactc tcttcgcctg cgtactggca tttatccctg
     9721 ttaaaaccgt ccgttatatc gcgctgacgt tgctgttttt cgtttggggc atattatcag
     9781 caaagcaaat tttgttggca ggagaaacct taactggcgc gacgcaggat gcaattgttg
     9841 agatcactgc tactgacggc atgaccactc attacggtca aattacttat ctacaaggtc
     9901 gacgtatatt ccctgcgcca ggccttgtgc tgtatggcga atatcttccg caagcggttt
     9961 gtgccggaca actgtggtca atgaaactca aagttcgtgc cgttcatggt caacttaatg
    10021 atggcggctt tgatagccag cgttatgcca ttgcccagca tcagccgctc accggccgct
    10081 ttctgcaggc aagtgttatt gaaccgaatt gtagcctgcg tgcacagtat ctggcgtcac
    10141 tacaaacaac gctgcaaccc tatccgtgga atgcggttat tcttggttta ggtatggggg
    10201 aacggttatc cgtccctaaa gaaatcaaaa atatcatgcg tgatactgga acggcgcatt
    10261 taatggcgat atcgggattg cacatcgctt ttgcggcgtt gctggctgcc ggactcattc
    10321 gcagtggaca aatttttctg cctgggcgct ggatccactg gcaaatgcca ttaattggcg
    10381 gaatctgctg tgctgctttt tatgcctggc tgacgggaat gcaacctcct gcattgcgta
    10441 ccgttgtggc gcttgctacg tggggaatgc ttaagttaag tgggcgacag tggagtggct
    10501 gggatgtatg gatatgttgt ctggcggcaa ttttgctgat ggatcctgtt gccattctct
    10561 cgcaaagttt atggctctct gccgctgcgg tcgcggcatt gatattttgg tatcagtggt
    10621 ttccctgtcc tgagtggcaa ctgccgccgg tattgcgtgc agttgtttcc ctcattcatt
    10681 tgcaactggg aatcacactt ctgcttatgc ccgtgcaaat cgtcatattt catggcatta
    10741 gtctgacctc gtttattgca aatctattag caattccctt ggtgacattt atcacggttc
    10801 cgctgatcct cgccgcgatg gttgtgcatt taagcgggcc gttaatcctg gagcaagggt
    10861 tatggtttct tgccgaccgg tctttggctt tacttttctg ggggttaaag agtttgccag
    10921 aagggtggat caacattgct gaacgttggc aatggctatc attttcccca tggttcttac
    10981 tggtggtatg gcgattaaac gcctggcgaa cgttgccagc aatgtgtgtg gctgtaggct
    11041 tgctgatgtg ctggccgctg tggcaaaaac ctcgacctga cgagtggcaa gtgtacatgc
    11101 ttgatgtcgg gcaagggctg gcaatggtga tagccagaaa cggcaaagcg attctctatg
    11161 acacgggact ggcctggccc gaaggggata gtgggcaaca actgattatc ccctggctcc
    11221 actggcataa tcttgaaccg gaaggcgtta ttctgagtca tgaacatctg gatcaccggg
    11281 gagggctgga ctcaatattg cacacatggc cgatgttatg gatcagaagt ccgttaaact
    11341 gggaacatca tcagccctgt gtgcgtggcg aagcgtggca atggcaagga ttgcgtttca
    11401 gcgtgcactg gcctttacaa gctagcaacg ataaaggaaa taaccattcc tgtgtggtta
    11461 aggttgatga cgggacgaat agcattcttc taaccggtga tattgaagtc cccgctgaac
    11521 aaaagatgct aagccgttac tggcagcaag tgcaggcaac attgcttcag gtacctcacc
    11581 atggcagtaa tacctcatca tcgttgccat taattcagcg agtgaatgga aaagtggcac
    11641 tcgcatcggc atcgcgctat aacgcatggc gactgccctc taacaaagtt aagcatcgct
    11701 atcaacaaca gggttatacg tggcttgata ctcctcatca ggggcaacta acggtcaatt
    11761 tttcagcaca aggctggctg attagcagcc tcagggagca aattttacct cgttggtatc
    11821 atcagtggtt tggcgtgcca gtggataacg ggtagaatat gcggctattt caacaaatgc
    11881 tggttttttg aatgcataac gacaaagatc tctctacgtg gcagacattc cgccgactgt
    11941 ggccaaccat tgcgcctttt aaagcgggtc tgatcgtggc gggcgtagcg ttaatcctca
    12001 acgcagccag cgataccttc atgttatcgc tccttaagcc acttcttgat gatggctttg
    12061 gtaaaacaga tcgctccgtg ctggtgtgga tgccgctggt ggtgatcggg ctgatgattt
    12121 tgcgtggtat caccagctat gtctccagct actgtatctc ctgggtatca ggaaaggtgg
    12181 taatgaccat gcgtcgccgc ctgtttggtc acatgatggg aatgccggtt tcattctttg
    12241 acaaacagtc aacgggtacg ctgttgtcac gtatcaccta cgattccgaa caggttgctt
    12301 cttcctcttc cggcgcactg attactgttg tgcgtgaagg tgcgtcgatc atcggcctgt
    12361 tcatcatgat gttctattac agttggcaat tgtcgatcat tttgattgtg ctggcaccga
    12421 ttgtttcgat tgcgattcgc gtagtatcga agcgttttcg caacatcagt aaaaacatgc
    12481 agaacaccat ggggcaggtg accaccagcg ctgaacaaat gctgaaaggc cataaagaag
    12541 tattgatttt cggtggtcag gaagtggaaa cgaaacgctt cgataaagtc agcaaccgaa
    12601 tgcgtcttca ggggatgaaa atggtttcag cctcttccat ctctgatccg atcattcagc
    12661 tgatcgcctc tttggcgctg gcgtttgttc tgtatgcggc gagcttccca agtgtcatgg
    12721 atagcctgac tgccggtacg attaccgttg ttttctcttc aatgattgca ctgatgcgtc
    12781 cgctgaaatc gctgactaac gttaacgccc agttccagcg cggtatggcg gcttgtcaga
    12841 cgctgtttac cattctggac agtgagcagg agaaagacga aggtaagcgc gtgatcgagc
    12901 gtgcgactgg cgacgtggaa ttccgcaacg tcacctttac ttatccggga cgtgacgtac
    12961 ccgcattgcg taacatcaac ctgaaaattc cggcagggaa gacggttgct ctggttggac
    13021 gctctggttc aggtaaatca accatcgcca gcctgatcac gcgtttttac gatattgatg
    13081 aaggcgaaat cctgatggat ggtcacgatc tgcgcgagta taccctggcg tcgttacgta
    13141 accaggttgc tctggtgtcg cagaatgtcc atctgtttaa cgatacggtt gctaacaaca
    13201 ttgcttacgc acggactgaa cagtacagcc gtgagcaaat tgaagaagcg gcgcgtatgg
    13261 cctacgccat ggacttcatc aataagatgg ataacggtct cgatacagtg attggtgaaa
    13321 acggcgtgct gctctctggc ggtcagcgtc agcgtattgc tatcgctcga gccttgctgc
    13381 gtgatagccc gattctgatt ctggatgaag ctacctcggc tctggatacc gaatccgaac
    13441 gtgcgattca ggcggcactg gatgagttgc agaaaaaccg tacctctctg gtgattgccc
    13501 accgcttgtc taccattgaa aaggcagacg aaatcgtggt cgttgaggat ggtgtcattg
    13561 tggaacgcgg tacgcataac gatttgcttg agcaccgcgg cgtttacgcg caacttcaca
    13621 aaatgcagtt tggccaatga tcgaaaaaat ctggtctggt gaatcccctt tgtggcggct
    13681 attgctgcca ctctcctggt tgtatggcct ggtgagtggc gcgatccgtc tttgctataa
    13741 actaaaactg aagcgcgcct ggcgtgcccc cgtaccggtt gtcgtggttg gtaatctcac
    13801 cgcaggcggc aacggaaaaa ccccggtcgt tgtctggctg gtggaacagt tgcaacagcg
    13861 cggtattcgc gtgggggtcg tgtcccgggg atatggtggt aaggctgaat cttatccgct
    13921 gttattgtcg gcagatacca ccacagcaca ggcgggtgat gaacctgtgt tgatttatca
    13981 acgcactggc gcgcccgttg cggtttctcc cgtgcgttct gatgcggtaa aagccattct
    14041 ggcgcaacac cctgatgtgc agatcatcgt aaccgacgac ggtttacagc attaccgtct
    14101 ggcgcgtgat gtggaaattg tcgttgttga tggtgtgcgt cgctttggca atggctggtg
    14161 gttgccggcg gggccaatgc gtgagcgagc ggggcgctta aagtcagttg atgcggtaat
    14221 cgtcaacggc ggtgtccccc gcagcggtga aatccccatg catctgctgc cgggtcaggc
    14281 ggtgaattta cgtaccggta cgcgttgtga cgttgctcag cttgaacatg tggtggcgat
    14341 ggcagggatt gggcatccgc cgcgcttttt tgccacgctg aagatgtgcg gcgtacaacc
    14401 ggaaaaatgt gtaccgctgg ccgatcatca gtctttgaac catgcggatg tcagcgcgtt
    14461 ggtaagcacc gggcaaacgc tggtaatgac tgaaaaagat gcggtgaaat gccgggcctt
    14521 tgcagaagaa aattggtggt atttgcccgt tgacgcacag ctttcaggtg atgaaccagc
    14581 gaaactgctt gcgcaactaa cctcgctggc ttctggcaac tagttacgcc gcggcagcgt
    14641 tcgattgatg gagtcatgaa tgtcgctgcc gcacctctcc cttgctgatg cgcgtaatct
    14701 tcaccttgcc gcgcaaggcc tgttaaacaa accccgccgt cgagcgtcgt tggaggatat
    14761 tccggcaacg atctcccgca tgtccttgct gcaaatcgat accatcaata ttgttgcccg
    14821 tagcccatat ctggtgcttt tcagtcgtct gggaaattat cctgcccagt ggctggatga
    14881 gtctctggcg cgtggcgaat taatggaata ctgggcgcat gaagcctgct ttatgccacg
    14941 cagcgacttt cgtcttattc gccaccgcat gctggcacct gaaaaaatgg gctggaaata
    15001 caaagacgcc tggatgcagg aacatgaggc ggaaattgca cagttaattc agcatattca
    15061 tgataagggg ccggtacgtt cagccgattt tgagcatcct cgtaaaggtg caagcggctg
    15121 gtgggaatgg aagccgcata aacggcatct ggaaggttta tttactgccg gaaaggtgat
    15181 ggtgattgaa cggcgcaact tccagcgcgt ttatgattta acccaccgtg tcatgcctga
    15241 ctgggatgat gagcgcgatc tcgtttcgca aacagaagca gaaatcatca tgctggataa
    15301 cagtgcgcgt agcctgggaa tattccgcga acagtggctg gcagattact atcggctgaa
    15361 acgtccggca ctggcagcgt ggcgcgaagc gagggctgaa cagcggcaaa tcattgctgt
    15421 gcatgttgaa aaattgggca atctttggct gcatgctgat ttgctgccgc tactcgagcg
    15481 agcgctggcc ggaaagctca ctgcaacgca cagcgcggta ctttcgcctt ttgatcctgt
    15541 tgtctgggat cgcaaacgcg cagagcagct ttttgatttt agctaccggc tggagtgcta
    15601 taccccagcg ccgaaacgcc agtatggcta ttttgttctg ccgttattac atcgtgggca
    15661 attagttggg cgaatggatg ccaaaatgca tcgccagaca ggcatccttg aagttatctc
    15721 tctgtggtta caggaaggta ttaaaccaac gacaacgctg caaaaagggt tacgtcaggc
    15781 gattactgat ttcgctaact ggcagcaggc aacgcgggtg acattaggac actgcccgca
    15841 aggtctcttt acggattgcc gcaccggctg ggaaatagac cccgtcgcat aaatgattat
    15901 gataagattt atgcattcat aacccgatcc atctggagga actatggatc atcgtctgct
    15961 tgaaatcatt gcctgcccgg tttgcaacgg aaaactttgg tataaccagg aaaaacaaga
    16021 gcttatttgc aaactggaca acctggcttt cccgctgcgc gatggcattc cagtgttgct
    16081 ggaaacggaa gcccgcgtgc tgactgctga tgagagtaaa tcatgagttt tgtggtcatt
    16141 attcccgcgc gctacgcgtc aacgcgtctg cccggtaaac cattggttga tattaatggc
    16201 aaacccatga ttgttcatgt tcttgaacgt gctcgcgaat caggtgccga gcgcatcatc
    16261 gtggcaaccg atcatgagga tgtcgcccgc gccgttgaag ccgctggcgg tgaagtatgt
    16321 atgacgcgcg ccgatcacca gtcagggaca gaacgcttgg cggaagttgt cgaaaaatgc
    16381 gcgttcagcg acgacacggt gatcgttaat gtgcagggtg atgaaccgat gatccctgcg
    16441 acaatcattc gtcaggttgc tgataacctc gctcagcgtc aggtgggtat ggcgactctg
    16501 gcggtgccaa tccacaatgc ggaagaagcg tttaacccga atgcggtgaa agtggttctc
    16561 gacgctgaag ggtatgcact gtacttctca cgcgctacca ttccgtggga tcgtgatcgt
    16621 tttgcaaaag gccttgaaac cgttggcgat aacttcctgc gtcatcttgg tatttatggc
    16681 taccgtgcag gctttatccg tcgttacgtc acctggcagc caagcccgtt agaacacatc
    16741 gaaatgttag agcagcttcg tgttctgtgg tacggcgaaa aaatccacgt tgctgtcgcg
    16801 catgaagttc ctggcacagg tgtggatacc cctgaagatc tcaagcgcgt tcgcgctgaa
    16861 atgcgctaat ttcacttcac gacacttcag ccaattttgg gaggagtgtc gtaccgttac
    16921 gattttcctc aatttttctt ttcaacaatt gatctcattc aggtgacatc ttttatattg
    16981 gcgctcatta tgaaagcagt agcttttatg agggtaatct gaatggaaca gctgcgtgcc
    17041 gaattaagcc atttactggg cgaaaaactc agtcgtattg agtgcgtcaa tgaaaaagcg
    17101 gatacggcgt tgtgggcttt gtatgacagc cagggaaacc caatgccgtt aatggcaaga
    17161 agctttagta cgcccggaaa agcccgacaa ctggcatgga aaaccaccat gctggcaaga
    17221 agtgggactg tccgtatgcc gactatttat ggtgtgatga cgcatgaaga acaccccggc
    17281 cctgatgtcc tgctactgga gcggatgcgt ggtgtttcgg tggaggcacc agcccgaaca
    17341 ccagaacgtt gggaacagct caaagaccaa atcgttgaag ccttactggc ctggcaccgt
    17401 caggacagtc gcggttgcgt cggcgcggtc gacaatactc aggaaaattt ctggccctca
    17461 tggtaccggc aacatgttga agtgctatgg accacgctca atcagttcaa taacaccggt
    17521 ctgacgatgc aggataagcg gatcctgttt cgcactcgcg aatgtctccc ggcattattt
    17581 gaaggcttta acgacaattg tgtgttgatt cacggtaact tctgtttacg cagcatgctg
    17641 aaagattcgc gcagcgatca gttactggcg atggtcgggc cgggactaat gctttgggca
    17701 ccgcgagaat acgaactgtt ccgactaatg gataattctc tggcggaaga tttgctctgg
    17761 agttacctgc aacgcgcgcc agtggcggag tcgttcatct ggcggcgttg gttgtatgtg
    17821 ttatgggatg aagttgcgca actggttaat accggacgat ttagtcggcg caacttcgat
    17881 ctggcatcaa aatcactctt gccgtggctc gcctgacgga cctttcagcc actgccagat
    17941 gcggccaagc gtttcatagc caacacgatc gctatgcatc agccagaccg gagaagggat
    18001 tgcccgttcc cacggattaa gcggcgagtc gatggccaac tgattcgccg gggctggtag
    18061 cggatttaac ccttcctgct gaaaaaagat catcgcccgc ggcagatgtg atgcggaggt
    18121 caccagcagg aaaggggcgt caccaatcgc ctgtttcact gccgcagctt cttcttcggt
    18181 atcttttggc aaatccaggg tgataatttg ctcgcgcggt acgcccagcg attgcgcaac
    18241 tctggcacct acttccgctg tactcaccgt attggttttt gctacgcctc ccgtgaagat
    18301 cagttttgaa cccggattat cgcgccataa acggataccc tcattcaggc gtggcagact
    18361 gttattgatt aaattagagc tcggtgccca ctgcgggttc caggtataac cacctccgag
    18421 caccacgata tagtccactt tttgcgaatt attccatgtc ggataggtgc tttcgatggg
    18481 acgcagcagg cggtcggcta ccggttgcaa actcaataac aagagtgaca gccacccaat
    18541 gctaataaaa atcttcccgg ttttttgaaa acggctaaac cacagcagcg ccaggccagc
    18601 gccaataatt aacaacatca gcggaagtgg cagcaacata ttgccaatca cttttttcag
    18661 tgtaaaaagc atcctttctg gttccttttt taaccatata gcaagggatc gccagcgata
    18721 ttacaccaga cgggttcatt ctcgccgtgg ctgtgacaaa atagcgtctt tatcagaaaa
    18781 cgtcttagcc aaccggtgga gaaagcaatg caggatcgca attttgatga tattgcggaa
    18841 aagttttccc gtaacattta cggcaccacc aaagggcagc ttcgacaggc tattctgtgg
    18901 caggatctcg atcgcgtgct ggcggaaatg ggtccgcaaa aactgcgtgt gctggatgcg
    18961 ggcggtggag aagggcagac cgcaatcaaa atggccgagc gcgggcatca ggtcatttta
    19021 tgcgatcttt ctgcgcagat gatcgaccgc gcaaaacagg cggcagaagc aaaaggtgtg
    19081 agcgacaaca tgcaatttat acattgcgcc gctcaggatg ttgcttcgca tttggaaacg
    19141 cccgttgatc tgatattgtt ccatgcggtg ctcgagtggg tggctgatcc ccgcagcgta
    19201 ttgcagaccc tgtggtcagt gttgcgtcca ggcggcgtgt tgtcgttaat gttctacaat
    19261 gcgcatggtt tgttgatgca taacatggtc gccgggaatt ttgattacgt gcaggcggga
    19321 atgccgaaaa agaaaaaacg gacgctttcg ccagattatc cacgcgaccc ggcgcaggtt
    19381 tatctgtggc tggaagaagc tggttggcaa attatgggta agacaggcgt tcgcgtgttt
    19441 catgattatc tgcgcgagaa gcaccagcag cgcgactgct atgaagcatt acttgaatta
    19501 gaaacgcgtt attgccgtca ggaaccgtat attaccctgg ggcgttatat tcatgtcacc
    19561 gcgcgcaaac cgcagagcaa ggataaagta tgagtgaatt ttcccagaca gtccccgaac
    19621 tggttgcctg ggccagaaaa aatgacttct ccatctcgct gccggtagac cgactctctt
    19681 ttctgctggc ggttgccacg ctgaacggcg agcgtctgga tggtgagatg agtgaaggcg
    19741 agctggtgga tgcattccgc catgtgagtg atgcgtttga gcaaaccagc gaaaccatcg
    19801 gcgtgcgcgc caataacgcg atcaacgaca tggtgcgtca acgtctgctg aaccgcttta
    19861 ccagcgagca ggcagaaggg aacgcaattt accgtctgac gccgctcggc atcggcatta
    19921 ctgactatta catccgtcag cgcgagtttt ctacgctgcg cctttctatg cagttgtcga
    19981 ttgtggcggg tgagctcaaa cgcgcagcag atgccgccga agagggcggt gatgaatttc
    20041 actggcaccg taatgtctat gcgccactga aatattcggt agcagaaatt ttcgacagta
    20101 tcgacctgac gcaacgtctg atggacgaac agcagcagca ggtgaaggac gatatcgccc
    20161 agttgctgaa caaagactgg cgggcggcga tttccagctg tgaattgttg ctttcggaaa
    20221 cttccggaac gctgcgtgaa ttgcaggata cactggaagc ggcaggcgac aaattgcagg
    20281 ctaatctgtt gcgcattcag gatgcgacga tgactcatga cgatctgcat tttgtcgatc
    20341 gtctggtgtt cgatctgcag agcaaactcg accgtattat cagttggggc cagcaatcca
    20401 tcgacttgtg gattggctac gaccgccacg tacacaaatt tattcgtacc gcgatcgata
    20461 tggataaaaa ccgcgtcttt gctcagcggt tacgtcagtc ggtacaaacc tattttgatg
    20521 agccgtgggc gctaacttat gccaatgccg atcgtctgct ggatatgcgt gacgaagaga
    20581 tggcactgcg cgatgaagaa gtgactgggg aacttcctga ggatctggaa tacgaagagt
    20641 ttaacgagat ccgcgaacag ctggcggcga tcatcgaaga acaacttgct gtgtacaaaa
    20701 ccagacaagt gccgctggat cttggtctgg tggtacgcga atatctgtca cagtatccgc
    20761 gtgcacgtca ctttgacgtt gcgcgcattg ttattgatca ggcggtacgt cttggcgtag
    20821 cgcaagcaga tttcaccgga ctgccagcga aatggcagcc gattaatgat tacggagcca
    20881 aggtacaggc gcatgtcatc gacaaatatt gaacaagtga tgccggttaa gctggcgcag
    20941 gcgctggcga atccgttatt tccggcgctg gacagcgcct tacgttcagg ccgccatatt
    21001 ggcctcgacg aactggataa tcatgcattc ctgatggatt ttcaggaata tctggaagag
    21061 ttttacgcgc gttataacgt tgagcttatt cgtgcaccgg aagggttctt ctatttacgt
    21121 ccacgttcca ccacgctgat ccctcgttcc gtcttgtcgg aactggatat gatggtcggg
    21181 aaaatcctct gctatctcta tctcagcccg gaacggctgg cgaatgaggg gatttttact
    21241 caacaggaac tgtacgacga actgctcacc ctggccgatg aagcgaaact gctgaaactg
    21301 gtgaacaacc gttcaaccgg ttcagacgtt gaccgtcaga agttgcagga aaaagtacgt
    21361 tcttcgctca accgtctgcg tcgtttaggc atggtgtggt ttatgggcca cgacagcagc
    21421 aagttccgca ttaccgaatc ggtgttccgc ttcggagccg atgtgcgtgc tggcgacgat
    21481 ccccgtgaag cacagcgtcg cctgattcgt gatggcgaag caatgccgat tgaaaatcat
    21541 ctgcaactca acgatgaaac cgaagagaat cagccagata gcggagagga agaataatga
    21601 ttgaacgcgg taaatttcgc tcactgacgc tgattaactg gaacggcttt tttgcccgaa
    21661 cttttgacct tgacgagctg gtcacgacgc tttctggcgg taacggggcg ggtaaatcca
    21721 ccaccatggc ggcgttcgtt acggcgctga tccccgacct gaccctgctg catttccgta
    21781 acactacgga agccggggcc accagcggtt cgcgcgataa aggtctgcac ggtaagctga
    21841 aagcgggtgt ctgttattcg atgctcgaca ccattaactc gcgccaccag cgcgtggtgg
    21901 tcggtgtgcg tctgcaacag gttgccggac gcgatcgtaa agtggatatc aaaccatttg
    21961 ccattcaggg actgccgatg tcggtgcagc cgacacagct ggtgaccgaa actctgaacg
    22021 aacgccaggc gcgcgtgctg ccgcttaacg agctgaaaga caagctcgag gcgatggaag
    22081 gcgtgcagtt taaacagttc aactccatta ccgattacca ctcgctgatg ttcgatctgg
    22141 gcatcatcgc gcgtcgtctg cgctccgcat ctgaccgtag caaattctat cgtctgatcg
    22201 aagcttcgct gtatggcggg atctccagtg ccattacccg ttctctgcgc gactacctgt
    22261 tgccggaaaa cagcggcgtg cgtaaagcgt tccaggacat ggaagcggcg ctgcgtgaaa
    22321 accgcatgac gctggaagcg attcgtgtca cccaatcgga ccgtgacctg tttaagcatc
    22381 tgatcagcga agctaccaac tacgtggcgg cggactacat gcgtcacgcc aacgagcgcc
    22441 gtgtccatct cgacaaagcg ctggagtttc gtcgcgagct acatacttcg cgtcagcaac
    22501 tggcggctga gcagtacaaa cacgtcgata tggcgcgtga gctggcagag cacaacggtg
    22561 ccgaaggcga tctggaagcg gattatcagg cggccagcga tcacctgaac ctggtgcaaa
    22621 ccgcactgcg tcagcaggag aagatcgaac gctacgaagc ggatctcgat gagctgcaga
    22681 tccgtctgga agagcaaaac gaagtggtgg cagaagccat cgaacgccag gaagagaacg
    22741 aggctcgtgc ggaagctgcc gaactggaag tggacgagct gaaaagccag cttgctgact
    22801 accagcaggc gctggacgtc cagcaaacgc gcgcgatcca gtataaccag gcgattgctg
    22861 cgcttaatcg tgccaaagaa ctgtgccatc tgccggactt aaccgccgac agcgccgccg
    22921 aatggctgga aaccttccag gcgaaagagc tggaagcgac tgaaaaaatg ctctctcttg
    22981 agcagaaaat gagcatggcg caaaccgcgc acagccagtt tgagcaggct tatcagctgg
    23041 tggtggcaat caacggcccg ctggcgcgta acgaggcgtg ggatgtcgcc cgcgaactgc
    23101 tgcgcgaagg ggtcgagcag cgtcacctgg cagatcaggt tcaaccgttg cggatgcgct
    23161 taagtgaact ggaacagcgt ctgcgcgagc agcaagaagc cgaacgcctg ctggcagatt
    23221 tctgcaaacg tcagggcaag aattttgata tcgacgaact ggaagccctg catcaggagc
    23281 tggaagcgcg tattgcctct ctttccgata gcgtgtctaa cgcccgcgaa gagcgtatgg
    23341 cactgcgcca ggagcaggaa cagctgcagt ctcgcattca gagtttgatg cagcgtgcac
    23401 cggtttggct ggcagcgcaa aacagtctca accagttgag tgaacagtgc ggcgaagagt
    23461 tctcctccag ccaggacgtc actgaatatc tgcaacagtt gctggagcgt gagcgcgagg
    23521 cgattgttga acgcgatgaa gtaggcgcgc gcaaaaacgc cgtcgatgaa gagatcgaac
    23581 gtttaagcca gcctggcggc tctgaagatc agcgtctgaa cgcgctggcg gagcgttttg
    23641 gtggtgtgct gctgtcagaa atttatgatg acgttagcct ggaagatgcg ccgtacttct
    23701 cagcgctgta tggcccgtca cgccacgcca tcgtggtgcc agatctatca caggtaactg
    23761 aacacctgga gggcttgacc gattgcccgg aagatctcta tttgatcgaa ggggatccgc
    23821 agtcattcga tgacagcgtg ttcagcgttg atgagctgga aaaagcggta gtggtgaaaa
    23881 tcgccgatcg tcagtggcgt tattcacgtt tcccggaagt gccgctgttt ggtcgtgctg
    23941 cgcgtgaaag ccgtattgaa agcctccatg ccgagcgtga agtgctttcc gaacgcttcg
    24001 ccacgctctc ctttgacgta cagaaaaccc agcgtctgca tcaggcgttc agccgcttta
    24061 tcggtagtca tctggcggtg gcgtttgagt ctgacccgga agcagaaatc cgtcaactga
    24121 acagccgtcg cgtcgaactg gagcgggcgt tgagtaatca tgaaaatgat aaccagcagc
    24181 agcgtattca gtttgagcag gcgaaagaag gcgttacggc gctgaaccgc attctgccgc
    24241 gtctcaacct gttggctgat gacagcctgg cggatcgcgt cgatgaaatc cgcgaacgtc
    24301 tggatgaagc ccaggaagcc gcgcgttttg ttcagcagtt tggcaatcaa ctggcgaaac
    24361 tggaaccgat cgtttcggta ttgcagagcg acccggaaca gttcgaacag ttaaaagaag
    24421 attacgcgta ctctcagcag atgcagcgcg atgcccgtca gcaggcgttt gccctgacgg
    24481 aagtggtgca gcgtcgtgcg cactttagct attctgactc ggcagaaatg cttagcggta
    24541 acaacgatct caacgaaaaa ctgcgtgaac gtctggaaca ggcggaagcg gagcgtaccc
    24601 gcgctcgcga agcgttgcgc ggtcacgcag cgcagttgag tcagtacaac caggtgctgg
    24661 cttcgctgaa aagttcttac gacaccaaaa aagagctact caacgatctg caacgtgaat
    24721 tgcaggatat cggcgtgcgt gctgatagcg gggcagaaga gcgggcacgt attcgccgtg
    24781 acgagctgca tgcgcaactg agcaataacc gttcgcgccg caaccaactg gaaaaagcgc
    24841 tcaccttctg cgaagctgag atggacaacc tgacccgcaa actgcgcaag ctggagcgtg
    24901 attactttga gatgcgcgag caggtagtga ccgcgaaagc gggctggtgt gcagtcatgc
    24961 gcatggtgaa agataacggc gttgagcgcc gcttacaccg tcgtgagctg gcttatctct
    25021 ccgctgatga tttgcgttcc atgtcggata aggcgttagg tgcgctgcgt ctggcggtgg
    25081 cggataacga acatctgcgc gacgtgctgc gcatgtcgga agatccgaaa cgtccggagc
    25141 gtaaaattca gttcttcgtg gcggtttatc agcatctgcg tgaacgtatt cgtcaggata
    25201 ttattcgtac cgatgatccg gtggaagcta tcgaacagat ggagatcgaa cttagccgtc
    25261 tgaccgaaga gttaacctcc cgtgaacaga aactggcgat cagttcccgc agcgtggcga
    25321 acatcattcg caaaaccatt cagcgcgaac agaatcgtat ccgtatgctc aaccaggggt
    25381 tgcagaacgt atcgtttggt caggtgaaca gcgtacgtct gaacgtgaac gtgcgtgaaa
    25441 cgcacgccat gctgttggat gtgctctctg aacagcacga gcagcatcag gatctgttta
    25501 acagcaaccg tctgaccttc tcggaagcgc tggcgaaatt gtatcagcgt cttaatccgc
    25561 agattgatat ggggcagcgc acgccgcaga ccattggcga agaactgctg gattaccgca
    25621 actatctgga aatggaagtt gaggttaacc gtggttccga tggctggttg cgcgcagagt
    25681 ctggtgcatt gtcgaccggt gaggcgattg gtaccggtat gtcgattctg gtgatggtgg
    25741 tacaaagctg ggaagatgaa tctcgccgcc tgcgcggtaa agatatctct ccttgccgcc
    25801 tgctgttcct cgatgaagca gcgcgactgg atgctcgttc tatcgccacg ctgtttgaat
    25861 tgtgtgaacg tttgcaaatg cagctcatca tcgcagcgcc ggaaaatatc agcccggaga
    25921 aaggcaccac ctataaactg gtgcgtaaag tcttccagaa taccgaacac gttcacgtcg
    25981 tcggcctgcg aggatttgcg ccgcaactcc ctgaaacgct tccaggaagt gacgaagcgc
    26041 cttctcaggc gagttaaaat taaggcggca gcaatgccgc cttttctttt tccgaaaact
    26101 ccgtttctgc actaaaaaag tggcacatta cggcgcgctt ttctttaaac ttctttacat
    26161 taggttatgt aaaaacgtat cggcgtttat atactgaaga taagcctgat gagtaacagg
    26221 cttgctcgtc atactttcgt gagtattggc gttgtacagg caagtcgtaa aataacagcc
    26281 tggctattca gagtatgata aaaacagggg gcaagggatg ttgcttaata tgatgtgtgg
    26341 tcgtcggctg tcggcaatca gtttgtgcct ggccgtaaca ttcgctccac tgttcaatgc
    26401 gcaggccgat gagcctgaag taatccctgg cgacagcccg gtggctgtca gtgaacaggg
    26461 cgaggcactg ccgcaggcgc aagccacggc aataatggcg gggatcctgc cattgcctga
    26521 aggtgcggca gaaaaagccc gcacgcaaat cgaatctcaa ttacccgcag gttacaagcc
    26581 ggtttatctt aaccagcttc aactgttgta tgccgcacgc gatatgcaac ccatgtggga
    26641 aaaccgtgat gctgttaaag ccttccagca acagctggca gaggtggcga ttgccggttt
    26701 ccagccgcag tttaataaat gggtagagtt actgaccgat cctggtgtta acgggatggc
    26761 acgcgacgtg gtgctctctg atgcgatgat gggctatctc catttcattg caaatattcc
    26821 ggtcaaaggc actcgctggc tatatagcag taaaccttat gcgctttcaa cgccgccgct
    26881 ttcggtgatt aaccaatggc agcaggcgct ggataaaggt caattgccta cgtttgttgc
    26941 aggactggca ccgcagcatc cgcaatatgc ggcgatgcat gaatcgttac tggccttact
    27001 cagtgacacc aaaccgtggc cccaactgac cggcaaagca acgttgcgcc cagggcagtg
    27061 gagtaacgac gtaccggcgt tgcgcgaaat attgcaacgc acaggcatgt tggacggggg
    27121 gccgaaaatt actctacctg gcgatgacac gccaactgac gcggtagtca gcccatccgc
    27181 tgttactgtt gaaacagcag aaactaagcc gatggacaag caaacgacgt ctcgtagtaa
    27241 acctgcgcct gccgttcgtg ccgcctacga taatgaactg gtggaagccg ttaaacgttt
    27301 tcaggcatgg caaggattgg gggcagatgg tgctattggc ccggcaacgc gggactggtt
    27361 aaacgtaacg cccgcccagc gtgctggcgt gttggcactc aacatccagc gattgcgctt
    27421 gctgccaaca gagctttcta ccgggatcat ggttaacatt ccggcctatt cgctggtcta
    27481 ctatcagaac ggcaatcagg tgctggattc gcgagtcatt gtcggtcgcc ccgatcgcaa
    27541 aacgccgatg atgagcagtg cacttaacaa cgtagtggta aacccgccgt ggaacgtacc
    27601 accaactctg gcacgcaaag atattctgcc gaaagtgcgc aacgatccgg gatatctcga
    27661 aagccatggc tatacggtga tgcgcggctg gaacagcaga gaagcgattg acccatggca
    27721 ggttgactgg tctacaatca cggcctcgaa tttaccgttt cgcttccagc aggctccagg
    27781 cccacggaac tcgctggggc gctataaatt caatatgccg agttcagagg ccatttattt
    27841 gcatgacacg ccgaaccaca atctgttcaa gcgtgataca cgcgcattga gctcaggctg
    27901 tgtacgagtg aataaagctt ccgatctggc gaatatgctg ttgcaggatg caggctggaa
    27961 tgacaaacgt atttctgatg cgctgaagca gggtgataca cgttacgtca atattcggca
    28021 gtcgattccg gtgaatctct actacctgac ggcctttgtt ggtgcagatg gtcgtaccca
    28081 gtatcgtaca gatatttaca attatgatct gcctgcgcga tccagctcgc aaatcgtatc
    28141 gaaagcggaa caattaatca ggtaaatgaa gtagttcggg gaaatcagtt gtcgtaataa
    28201 ttagcatgat tgggggtggt tctctgcagc ccccgtcact gctggggttg agtcatcttg
    28261 acgtctgctt tacgggcggt taaggtgcct cttgtgcgcc agaagtgcat ataaacgata
    28321 acattgacct gtagacttga ttatcatgga caaattcgac gctaatcgcc gcaaattgct
    28381 ggcgcttggt ggcgttgcac tcggtgccgc catcctgccg acccctgcgt ttgcaacact
    28441 ctctacccca cgcccgcgca ttttgacact caataatctt cataccggag agtcaatcaa
    28501 agcggagttt ttcgatggca gaggctatat tcaggaagaa ttggcaaaac ttaaccattt
    28561 tttccgcgat taccgcgcga acaaaataaa gtccatcgac ccaggattat tcgaccagtt
    28621 gtatcgcctg caagggttat taggcacccg caaaccggtg caactcattt ccggttatcg
    28681 ttccattgat accaacaatg aactacgcgc ccgcagccgt ggagtagcga agaaaagcta
    28741 tcacactaaa ggccaggcga tggatttcca tattgaaggt atcgcgttaa gcaatattcg
    28801 caaagccgcg ttatctatgc gcgcaggtgg tgtaggatat tacccacgta gtaactttgt
    28861 gcatattgat accgggccag cacggcactg gtagtaattg cttaacgaaa caggggcagt
    28921 atgaactatc gtattattcc ggtcaccgca ttctcccaga actgttcatt gatctggtgt
    28981 gaacaaaccc gactggccgc actggtcgat cctggcggcg atgcggaaaa aatcaaacag
    29041 gaagttgatg ccagcggcct gacactgatg cagatcctgc tgacgcatgg tcatctggac
    29101 cacgttggcg cagcggcgga actggcgcaa cattacggcg tgccggtttt tggcccggaa
    29161 aaagaagatg agttctggct gcaaggcttg cctgcgcaaa gtcgtatgtt tggtctggaa
    29221 gagtgtcagc cgctgacgcc agatcgttgg ctgaaagaag gcgataccat cagcataggg
    29281 aatgtgactt tacaggtgtt acattgccct gggcatacgc cgggtcatgt cgtgtttttt
    29341 gatgatcggg caaagctgct gatttctggc gatgttattt tcaaaggcgg agtagggcgc
    29401 agtgacttcc cgcgtggcga tcataatcaa ctgatttctt caatcaaaga taaattgctg
    29461 ccactggggg atgacgtgac atttattccg ggtcacggac cattatccac acttggttat
    29521 gaacgcctgc ataatccctt cctgcaagac gaaatgcccg tctggtaagg cacacataaa
    29581 aaagcccgct tttcagcggg cttcattgtt tttaacgctt acagcactgc cacaatcgct
    29641 tcgcacagcg gagccatgtt atctggtgtc atccctgcca catttacgcg accagaagca
    29701 acagcatata cgccaaactc ttcgcgcaga cgcaacactt gttcttttgt cagaccactg
    29761 aaggagaaca tgccgttctg tttgatgata aagctgaagt cgcggtttgc ccctttttcc
    29821 tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc
    29881 tcttgttccc aaatcgcacg taatgcatcg ttgctcagga tggtggcaac aacagaagcg
    29941 ccgtgtgctg gagggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg
    30001 cgatcaacgg tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac
    30061 aggccaaagt ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa
    30121 gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca
    30181 aacagcggta accagccttt ctcaacggag agttgtgcca gtgtctgcca ttgttccagc
    30241 gtagggtcga taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc
    30301 tgagcttcgt tcaggctgtt aatcagtgca tcgaagtcaa gggtgtgatt ttccgcatca
    30361 taataagcgt attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc
    30421 cagcttgggt tgctcaccca cacacgctta acgctggtat tttttgccag gaagtcggca
    30481 gccacgcgta gtgcgccagt gccacccgga gtctgtgccg tgcgagcacg tttgtcattg
    30541 atcagggcgc tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg
    30601 ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt
    30661 ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg
    30721 ttaattttgc cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg
    30781 gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa
    30841 gtccgctatc agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag
    30901 gctgtttttg caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc
    30961 tgttttttcg gcatttaaca aagaggtgtg ctattagaac tggtaaacga tgcccacagc
    31021 aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta
    31081 gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt
    31141 caccagatca acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat
    31201 ggacggacgc agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt
    31261 gttggcgaag ccgccttcaa ttggcgtagc gttacgggtt tcaccgtagt tcgctgccag
    31321 gtagatgttg ttcgcgtcat acttcagacc agttgcccac tgttcagctt ttttaccctg
    31381 accgcggaat tcggcttcct gggcgtcggt acggtctgct gcaccgtaag cacctacgat
    31441 accaaagcct tcgaattcgt agctgataga gccaccgacg ccgtcgccgt tggaacgctc
    31501 aggaatacca gcacgttcgt ttttgcccag gtactgaacg gcaaagttca ggccgtcaac
    31561 cagaccgaag aagttggagt tacgataggt agcaacgccg cctacacgac caacgaagaa
    31621 gtcatcgctg tatgccgtgt caccgccaaa ctcaggcagc atatcggtgt aacccagtgc
    31681 gtcgtaaacc acaccgtagt tacgaccgta gtcgatagaa cccgcatcag cgaatttcag
    31741 acctgcgaat gccaaacgag ttttgttacc tttctgggca tcagcgcctt cagagttgtt
    31801 accctggaag ttatattccc actgaccata accggtcaga tcggaattga tttgtgtttc
    31861 ccctttaaaa ccaagacggg cataagtttt gtcgccgttt ccgccgtaac tgtttacacc
    31921 attgtcttta gaaaaataat gcagaccgac agctttaccg tacagatcta ctttgttgcc
    31981 atctttgtta tagatttctg cagcgtttgc agtacctgct actaacagag cagggacgat
    32041 cactgccaga atattgcgct tcatcattat ttattaccct catggttttt ttatgacacc
    32101 tgccactgcc gtcaataagt tctgtcaata aaaatttacg gaactattga tgagagtttg
    32161 gtgtctttat gtgtctgcag gcatctttcc attcaag
//
LOCUS       DAZGBC010000039        31630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGBC010000039 DAZGBC010000000
VERSION     DAZGBC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.039"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(455..3604)
                     /gene="acrB"
                     /locus_tag="ACHK1J_001841"
     CDS             complement(455..3604)
                     /gene="acrB"
                     /locus_tag="ACHK1J_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO7911118.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3627..4820)
                     /gene="acrA"
                     /locus_tag="ACHK1J_001842"
     CDS             complement(3627..4820)
                     /gene="acrA"
                     /locus_tag="ACHK1J_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO7911119.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4962..5609
                     /gene="acrR"
                     /locus_tag="ACHK1J_001843"
     CDS             4962..5609
                     /gene="acrR"
                     /locus_tag="ACHK1J_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO7911120.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5737..9099
                     /gene="mscK"
                     /locus_tag="ACHK1J_001844"
     CDS             5737..9099
                     /gene="mscK"
                     /locus_tag="ACHK1J_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO7911121.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKIKVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            9160..9258
                     /locus_tag="ACHK1J_001845"
                     /pseudo
     CDS             9160..9258
                     /locus_tag="ACHK1J_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9311..9472)
                     /gene="rsmS"
                     /locus_tag="ACHK1J_001846"
     CDS             complement(9311..9472)
                     /gene="rsmS"
                     /locus_tag="ACHK1J_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO7911122.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9486..10013)
                     /gene="priC"
                     /locus_tag="ACHK1J_001847"
     CDS             complement(9486..10013)
                     /gene="priC"
                     /locus_tag="ACHK1J_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO7911123.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            10083..10460
                     /gene="ybaN"
                     /locus_tag="ACHK1J_001848"
     CDS             10083..10460
                     /gene="ybaN"
                     /locus_tag="ACHK1J_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO7911124.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10613..11164
                     /gene="apt"
                     /locus_tag="ACHK1J_001849"
     CDS             10613..11164
                     /gene="apt"
                     /locus_tag="ACHK1J_001849"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO7911125.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            11293..13224
                     /gene="dnaX"
                     /locus_tag="ACHK1J_001850"
     CDS             11293..13224
                     /gene="dnaX"
                     /locus_tag="ACHK1J_001850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO7911126.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12557..12621
                     /gene="dnaX"
                     /locus_tag="ACHK1J_001850"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            13277..13606
                     /gene="ybaB"
                     /locus_tag="ACHK1J_001851"
     CDS             13277..13606
                     /gene="ybaB"
                     /locus_tag="ACHK1J_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO7911127.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13606..14211
                     /gene="recR"
                     /locus_tag="ACHK1J_001852"
     CDS             13606..14211
                     /gene="recR"
                     /locus_tag="ACHK1J_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO7911128.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            14321..16195
                     /gene="htpG"
                     /locus_tag="ACHK1J_001853"
     CDS             14321..16195
                     /gene="htpG"
                     /locus_tag="ACHK1J_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO7911129.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16376..17020
                     /gene="adk"
                     /locus_tag="ACHK1J_001854"
     CDS             16376..17020
                     /gene="adk"
                     /locus_tag="ACHK1J_001854"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO7911130.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            17152..18114
                     /gene="hemH"
                     /locus_tag="ACHK1J_001855"
     CDS             17152..18114
                     /gene="hemH"
                     /locus_tag="ACHK1J_001855"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO7911131.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(18111..19070)
                     /gene="aes"
                     /locus_tag="ACHK1J_001856"
     CDS             complement(18111..19070)
                     /gene="aes"
                     /locus_tag="ACHK1J_001856"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO7911132.1"
                     /translation="MKPENKLPVLDLISAEMKIVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNYADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            19222..20526
                     /gene="gsk"
                     /locus_tag="ACHK1J_001857"
     CDS             19222..20526
                     /gene="gsk"
                     /locus_tag="ACHK1J_001857"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO7911133.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20656..22332)
                     /gene="ybaL"
                     /locus_tag="ACHK1J_001858"
     CDS             complement(20656..22332)
                     /gene="ybaL"
                     /locus_tag="ACHK1J_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO7911134.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22570..23790)
                     /gene="fsr"
                     /locus_tag="ACHK1J_001859"
     CDS             complement(22570..23790)
                     /gene="fsr"
                     /locus_tag="ACHK1J_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO7911135.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            24008..25660
                     /gene="ushA"
                     /locus_tag="ACHK1J_001860"
     CDS             24008..25660
                     /gene="ushA"
                     /locus_tag="ACHK1J_001860"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO7911136.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARVLPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QLDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25697..26176)
                     /gene="ybaK"
                     /locus_tag="ACHK1J_001861"
     CDS             complement(25697..26176)
                     /gene="ybaK"
                     /locus_tag="ACHK1J_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO7911137.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26380..27174)
                     /gene="ybaP"
                     /locus_tag="ACHK1J_001862"
     CDS             complement(26380..27174)
                     /gene="ybaP"
                     /locus_tag="ACHK1J_001862"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO7911138.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTNDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27312..27653
                     /gene="ybaQ"
                     /locus_tag="ACHK1J_001863"
     CDS             27312..27653
                     /gene="ybaQ"
                     /locus_tag="ACHK1J_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7911139.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLALKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27868..30372)
                     /gene="copA"
                     /locus_tag="ACHK1J_001864"
     CDS             complement(27868..30372)
                     /gene="copA"
                     /locus_tag="ACHK1J_001864"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO7911140.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADSDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30634..31566
                     /gene="glsA"
                     /locus_tag="ACHK1J_001865"
     CDS             30634..31566
                     /gene="glsA"
                     /locus_tag="ACHK1J_001865"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO7911141.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         7768 a         8373 c         8265 g         7224 t
ORIGIN      
        1 taactataag ccactctttg caggttttca tcgcattatt acgaaaaatt acaattagga
       61 aggaagcggg acagggctca ctttttcata ctagcaccaa cccgcagcag gtaaaagcag
      121 tttgctcgaa ctgttcataa catttcagga ttatcgccag atgaaattgg tgacactaat
      181 accaggattg ctctgaatat gacgtaataa ccgaggaatg aataaagaat taccgcaaat
      241 aattaagaat agcctctaaa tgattatgga ttattaaatc tattagcgct acttaatata
      301 aattaactaa caatcagtat tttttatgaa ttttatccgt ggttaatact ggttttcgta
      361 tgagatcctg agttggtggt tcaattactc cttaatgttc gtaggttatg cataaaaaag
      421 gccgcttacg cggccttagt gattacacgt tgtatcaatg atgatcgaca gtatggctgt
      481 gctcgatatc ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa
      541 cgaagaagat tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg
      601 cgttctgcgc gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa
      661 acgccagcga ggtcatcagg atcggacgta aacgcatccg caccgcatca agcgtcgctt
      721 caatcagacc tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg
      781 cgttcttcgc cgacaaccca atggttgtga gcaggcctac ctggaagtaa acgtcattgg
      841 tcagcccacg gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca
      901 taacggagaa cggaatcgac cagctctcgt acagcgccgc cagacacagg aacacgacaa
      961 tcaacgaaat cgcgtacagt gaaggtgcct ggttgccgga gagacgttcc tgataggaca
     1021 tccccgtcca gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct
     1081 ccattgcttc accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca
     1141 ggccgttgta acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg
     1201 agaatggcac catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca
     1261 gcatacggta tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa
     1321 agtcgttcac atagctgccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag
     1381 aaacacccag cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt
     1441 ccagaccgtt tggacgtacg ctggtcaaca tatcagggtg cttcgctgct tctgcaagca
     1501 actggttacg cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa
     1561 agtcaaagcc ggttgcggta cccagttcca cgatcgcggg caggttaaag gcgaaaacca
     1621 tcgcatcttt gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt
     1681 tttcttcgcc cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct
     1741 gaccacgtcc cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt
     1801 ctttggtcag atagtaatgc gttacctcat tgagcacttt ctgtgtacgt tcctgcgttg
     1861 cacctgctgg cagctgaacc atggtcataa atacgccctg gtcctcatct ggcaagaagg
     1921 agcttggcag acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacca
     1981 ggtaacgccc tgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct
     2041 tctcgaacat gcggttaaac cagccgaaga agcctttttt accttccccg tgatcgcctt
     2101 tggcaatcgg tttcagcatg gtggcacaaa gagccggagt caggatcaac gctaccagta
     2161 ccgacagcgc cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag
     2221 aaccgccaaa gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca
     2281 gagcgccctg aatctgcccc atcgacttac gggtggcttc ttttggcggc aaaccttctt
     2341 ccgccatcac acgctcaacg ttttctacca caacgatagc gtcatccacc aacaggccga
     2401 tggcgagcac catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga
     2461 cggcaaaggt cccgagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga
     2521 agttctgcag gaacagatac ataaccagga acacgaggat gatcgcttcg accagcgttt
     2581 taaccacttc gtgaatagag attttcacga acggcgtggt gtcgtacggg taaacaattt
     2641 tcagacccga cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg
     2701 tatccagcgc gtttgcaccg gtcgccagct tgatccccag accggaagcc ggttggccgt
     2761 taaactctgc gatgatgtcg tagttctcac cacccagctc aatcttcgcc acatcacgca
     2821 gcagcacgcg ggaaccatcc tgattcactt tcagcaggat tttgccgaac tcttcagtag
     2881 aggtcagacg cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg
     2941 taccaccgag ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat
     3001 caaccggcgt tagctggaat ttgttcagct cattcgggtt catccagata cgcatcgcgt
     3061 actgtgaacc gaacaactga acgtcaccca cgcccgacgt acggctgatg gcatctttca
     3121 tattcgccgc cacatagtcg gagatatcct cctgcgtcat ggtgccatcg gtgttgataa
     3181 cgccgacaac catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgaa
     3241 cttcttgcgg cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga
     3301 tatccgcatc agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac
     3361 tgttagagga catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg
     3421 tcaccgtgtc ctgcactgtt ttcgcatcag cgccggggta ggaggcggag atcgttactg
     3481 ccggcggtgc aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg
     3541 ccaacatgat gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag
     3601 gcatgtctta acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt
     3661 gcggcttgct ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc
     3721 actttctgca gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc
     3781 cacttatcgc caatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg
     3841 ccaactacca gtacggtggc atcgccacgc ggcgtacggg ttacgccctg ttgcgggact
     3901 aaaatagcgt ttggattaag cccttcttcc agacgtgcgc gcacgaacat acccggcagc
     3961 agagtgtgat ccgggttcgg gaagatagcg cgtagggtga tagacccagt ggtctgatca
     4021 acggtaacgt cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg
     4081 atcagcgaca ctttggcttt gccgttctct tgtttcagcg tgccattcgc cagttcctgt
     4141 ttcaggcgca ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc
     4201 tgcacggttg ccagcgcagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc
     4261 gacttaccaa tgcgaccgct aatcggagag gtgactttgg tgtaagccag attgatccgc
     4321 gcagtttcaa cggcagcttt cgctgcagtt accgcagcat tcgcctgttg cgcatcagcc
     4381 agagcctgat cgtactcttg cttactgatg tactgagtac cgagcagttt ctgataacga
     4441 ttcaccgtca attgcgcgat attggctgca gcctgggctt tcgccagatc acctttcgca
     4501 ctgtcgtatg tcgcctgata ggtcgcagga tcaatctgat agagagagac acctgcttcg
     4561 atgtcgctac cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact
     4621 tctgcgatcc ggtaggcact ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca
     4681 gttttgactg ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg
     4741 tcgtcacatc ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta
     4801 aaccctctgt ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt
     4861 caaaagttaa taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc
     4921 acaaatgtat gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac
     4981 aagaagcgca agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc
     5041 agggggtatc atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg
     5101 caatctactg gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag
     5161 aatccaatat tggtgaacta gagcttgagt atcaggcaaa attccctggc gatccactct
     5221 cagtattaag agagatatta attcatgttc ttgaatccac ggtgacagaa gaacggcgtc
     5281 gattattgat ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc
     5341 aacaggcaca acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac
     5401 attgtattga agcgaaaatg ttgcctgcgg atttaatgac gcgtcgcgca gcaattatta
     5461 tgcgcggcta tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc
     5521 ttaaaaaaga agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca
     5581 cgcttcgtaa ccctgccact aacgaataac cctgaatctg actccaggat ttttcctgga
     5641 cattttcgtc gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt
     5701 caggtcgttc ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa
     5761 cgatcacggc attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag
     5821 aacacggcgt ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag
     5881 gcgcaacttg actcactaaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag
     5941 caggatctga cagatacatt agccaccctc gataaaatcg atcgcgtaaa agaagagacc
     6001 gttcagctac ggcaaaaagt cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg
     6061 ttaacagcac ttagcgatgt cgataacgac gaagaaacgc gcaaaattct gagcacgctt
     6121 tcgttgcgcc agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa
     6181 aacgatctag cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg
     6241 caaaatgcga tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg
     6301 actgatgtcg gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag
     6361 gcgttgctga atgcggagat tgaccagcag cgtaaaagcc tggaagggaa taccgtcttg
     6421 caggatacct tgcaaaagca acgtgattac gtgacggcga acagcgcccg tctggagcac
     6481 cagttacaac tgttgcaaga agcggtaaac agcaagcgcc tgactttaac cgaaaaaacg
     6541 gcgcaggaag ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaag
     6601 caggaactgg aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt
     6661 aatcagttga tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg
     6721 gaacgcaata ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc
     6781 ctttaccagc aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc
     6841 atcgcggatt tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag
     6901 agcgatgcgt tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt
     6961 cacgatgcgt tattgcaagt ggttgatatg cgtcgcgaat tgctggatca actcaacaaa
     7021 cagttgggta accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg
     7081 agtgtgtcga aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaacagtaac
     7141 cgtccaatgg actgggactg gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag
     7201 tcgatgaaaa ttaaggtgaa ctgggaaaaa gcctggcccg ccgtttttat cgctttcctc
     7261 gctggtttgc cgctgctgtt gattgccggg ctgatccact ggcgtctggg gtggctgaaa
     7321 gcgtatcaac aaaaactggc ttccgctgtg ggttccctgc gtaacgacag ccagctcaac
     7381 acaccaaaag cgatccttat cgacctgatc cgtgcgctgc cggtgtgcct gattattctc
     7441 gcggttggcc tgattctgtt gaccatgcag ctcaacatca gcgaactgct atggtcgttc
     7501 agtaaaaaac tggcgatttt ctggctggtg tttggcctgt gctggaaggt actggagaaa
     7561 aatggcgttg ccgtgcgtca cttcggcatg ccggagcagc agaccagcca ctggcgtcgg
     7621 caaattgtcc gcatcagtct cgcgttgctg cctatccatt tctggtctgt ggtggcagaa
     7681 ctttccccgc tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacctg
     7741 ctgctgattg ccttcctggt atggccaatg tgccgcgaaa gctggcgtga taaagagtcg
     7801 cacaccatgc gactggtcac cattaccgtg ctgtcgatta tcccgattgc attgatggtg
     7861 ctgactgcca cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc
     7921 gtttatctgg tgatcatctg gaacttgctc taccagacgg tattgcgcgg cttaagcgta
     7981 gcggcgcggc gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag
     8041 ggcgcagaag gtgccgaacc gccggaagaa cccaccattg cactggaaca ggttaaccag
     8101 cagacgctgc gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca
     8161 atttggtccg atttgatcac cgtgttcagc tatctcgaca gcattacgct gtggcattac
     8221 aacggtactg aagctggtgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt
     8281 gcgattatcg cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttgctggaa
     8341 gtgctggtgc tctcgcgact gaatatgcgc cagggcgcgt cgtatgccat tactaccatc
     8401 cttaactaca tcattattgc tgttggtgcg atgacggtgt tcggatcgct gggcgtctct
     8461 tgggataaac tccagtggct ggcagcagca ttatccgtag gtctcggttt tggtttacaa
     8521 gaaattttcg gtaacttcgt ctccggtttg atcattctat tcgaacgtcc ggtgcgtatt
     8581 ggcgatacgg taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg
     8641 acaacgatta ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc
     8701 gagcgtctga tcaactggtc gttgactgac actactacgc gtctggtgat ccgtctcggc
     8761 gtggcctatg gctccgatct ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag
     8821 cacccaaggg tgatgcacga accaatgccg gaagtcttct ttacggcgtt tggtgccagc
     8881 acgttggatc atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag ccgtactgtc
     8941 gatgagctga accgtactat cgatcagctg tgccgtgaaa acgacatcaa cattgccttt
     9001 aaccagcttg aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtaaaacgc
     9061 gactacaaag gcgatgaccc gacgccagcg gtagggtaat aacgaaaggg ggcgacattt
     9121 tgttgccccg agattgctaa caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc
     9181 ttatccggcc tacgaaaccg caaaaattca atatattgca ggatcggtgt aggcctgata
     9241 agcgtagcgc atcaggcagt tttgcgtttg cccgcaacct taggggacat ttagccaccc
     9301 catttatttc tcacttttcc gcctcatcat cgcgcgttaa tttcttttca taatcgcgtt
     9361 ttacaatatc cagcgcgcgc agcacggtgc gggctgggat ctgattttct tccagcagca
     9421 caatcaaatc gacggccagt ttgacatcgt cgggggcatt ttccagtgac atactctctc
     9481 cattgctagc gggttaaacg cgctaacctg ttttcgattt tttccagcgc atggcgacac
     9541 cgcgccaggc gcgcttcata ggcttccact tcacgatgca gcgtttgctg ttccacgaga
     9601 tcggtcaccc gcgccaggcg ggctctacgt tcggcaacca tctcacgtag ccgccgctca
     9661 aaatcctgat gctgaatacg tttacgctgc cagcgggcaa ttttcggtgg cgcactgtcc
     9721 cactcgcgca atgaccaggc tgtggcttca cgcgctatag cctccagttg cgccgccaga
     9781 tgttctgcca gccaggcgac ttgcggcagt tgctgttgct caactgcatg acgaagcgca
     9841 gccagattat cgcccgcctc atcgagacaa gcctgcagtg ttgtcgcacg ggtctgaaaa
     9901 aggtgcctgt caaagcgggc acttagcgtg gcaaattgcg ctaccggggc acaacgctgg
     9961 cgcagcgtgg cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga
    10021 cctcaatgaa aatgataatt gttatgctaa agtagccact ctgtaagctg acaacactca
    10081 atatgcaacg aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg
    10141 gcgtggtatt accggtatta ccgacgacgc cgtttatcct gctagcggcc tggtgctttg
    10201 cccgttcttc cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc
    10261 tacgtttctg gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc
    10321 ttattttgct cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca
    10381 tgttgctggt aattctcgcc tgtttgcttt tctatatgtg gcgaattccg gtgattgatg
    10441 aaaagcaaga aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt
    10501 acattctggc gttttcgagc acaggcgcag gcggtcaaag gttaaacaac tgttactttt
    10561 gatacgttta aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc
    10621 gactgcacag cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa
    10681 acccggcatt cttttccgcg atgtcaccag cttactggaa gacccgaaag cttacgctct
    10741 cagcatcgac ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac
    10801 cgaagcgcgt ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt
    10861 accggtccgt aaaccgggca aactgccgcg tgaaactatc agtgaaactt acgatctgga
    10921 atacggcacc gatcagctgg agatccacgt tgatgccatc aaaccgggcg acaaagttct
    10981 ggtggtggac gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg
    11041 tcgtctgggt ggtgaagtgg ctgacgctgc gttcattatc aacctgttcg atctcggcgg
    11101 cgaacagcgt ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca
    11161 ttaattatcg ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc
    11221 tcgccgttct gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt
    11281 cagagcctgc caatgagtta tcaggtctta gcccgaaaat ggcgcccaca aacctttgct
    11341 gacgtcgtcg gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt
    11401 attcatcatg cttatctttt ttccggtacc cgtggcgtcg gaaaaacctc tatcgcccga
    11461 ctgctggcga aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc
    11521 gataactgtc gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc
    11581 tcgcgcacca aagttgaaga cactcgcgac ctgctggata acgtccagta cgctccggcg
    11641 cgtggtcgtt tcaaagttta tctcatcgac gaagtgcata tgctgtcgcg ccacagcttt
    11701 aacgcactgt taaaaactct tgaagagccg ccggagcacg ttaagtttct gctggcgacg
    11761 accgacccac agaaattgcc ggtgacgatt ttgtcgcgct gtttgcaatt tcatctcaag
    11821 gcgctggatg tcgagcaaat tcgccatcaa cttgagcaca tcctcaatga agaacatatc
    11881 gctcacgagc cgcgggcgct gcaattgctg gcgcgcgccg ctgaaggcag cctgcgagat
    11941 gccttaagtc tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggcg
    12001 gtcagtgcga tgctgggtac gcttgacgac gatcaggcgc tgtcgctggt tgaagcgatg
    12061 gtcgaggcca acggcgagcg cgtaatggcg ctgattaatg aagccgctgc ccgtggtatc
    12121 gagtgggaag cgttgctggt ggaaatgctc ggcctgttgc atcgtattgc gatggtacaa
    12181 ctttcgcctg ctgcacttgg caacgacatg gccgccatcg agctgcggat gcgtgaactg
    12241 gcgcgcacca taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc
    12301 aaagaattac cgtatgcgcc ggaccgccgc atgggcgttg agatgacgct gctgcgcgcg
    12361 ctggcgttcc atccgcgtat gccgctgcct gagccagaag tgccacgcca gtcctttgca
    12421 cccgtcgcgc caacggcagt aatgacgcca acccaggtgc cgccgcaacc gcaatcagcg
    12481 ccgcagcagg caccgactgt accgctcccg gaaaccacca gccaggtgct ggcggcgcgc
    12541 cagcagttgc agcgcgtgca gggagcaacc aaagcaaaaa agagtgaacc ggcagccgct
    12601 acccgcgcgc ggccggtgaa taacgctgcg ctggaaagac tggcttcggt cacggatcgc
    12661 gttcaggcgc gtccggtgcc atcggcgctg gaaaaagcgc cagctaaaaa agaagcgtat
    12721 cgctggaagg cgaccactcc agtgatgcag caaaaagaag tggtcgccac accgaaggcg
    12781 ctgaaaaaag cgctggaaca tgaaaaaacg ccggaactgg cggcgaagct ggcggcagaa
    12841 gccattgagc gcgacgcgtg ggcggctcag gtgagtcaac tttcgctacc aaaactggtc
    12901 gaacaggtgg ccttaaatgc ctggaaagag gagagtgata acgcagtatg tctgcatttg
    12961 cgctcctctc agcggcattt gaacaaccgc ggtgcacagc aaaaactggc tgaagcgttg
    13021 agcatgttaa aaggttcaac ggttgaactg actatcgttg aagatgataa tcccgcggtg
    13081 cgtacgccgc tggagtggcg tcaggcgata tacgaagaaa aacttgcgca ggcgcgcgag
    13141 tccattattg cggataataa tattcagacc ctgcgtcgat tcttcgatgc ggagctggat
    13201 gaagaaagta tccgccccat ttgatcgtaa gcacagctta cgttcgtcat ccttaacgtg
    13261 attgagagag aaacctatgt ttggtaaagg cggtctgggt aacctgatga agcaagccca
    13321 gcagatgcaa gaaaaaatgc agaaaatgca ggaagagatc gcgcagctgg aagtcaccgg
    13381 cgaatctggc gcaggtctgg taaaagtgac catcaacggt gcacacaact gccgtcgcgt
    13441 agagatcgac ccgagcctgc tggaagacga caaagagatg ctggaagacc tggtggctgc
    13501 agcattcaac gacgcagcac gtcgtattga agaaacgcag aaagaaaaaa tggcctctgt
    13561 atcctccgga atgcagctgc cgcctggctt taagatgccg ttctgatgca aaccagcccg
    13621 ctgttaacac agcttatgga agcactgcgc tgtctgccgg gcgttggccc gaagtcggcg
    13681 cagcgtatgg cgttcacgct gcttcagcgc gatcgtagcg gcgggatgcg tctggcgcag
    13741 gcgctcaccc gggcgatgtc ggaaatcggc cactgcgccg attgccgcac tttcaccgaa
    13801 caggaagtct gtaacatctg ttcgaatccg cgtcgtcagg aaaacggtca aatctgcgtg
    13861 gtggagagtc cggcggacat ttacgccatt gagcagacgg ggcagttttc aggtcgttat
    13921 tttgtgttga tgggacatct gtcaccgctg gacggcatcg gtccggatga tatcggcctt
    13981 gatcgtctgg aacagcgtct ggcagaggaa aaaatcactg aagtgatcct cgccaccaac
    14041 cctacggttg aaggtgaagc taccgctaac tacattgccg agctttgcgc gcaatatgac
    14101 gtggaagcca gccgaatcgc tcatggcgta ccggttggcg gcgagctgga aatggtcgat
    14161 ggcaccacgc tgtcacactc ccttgccggg cgtcataaga ttcgttttta agcaaacgag
    14221 agcaggatca cctgctctcg cttgaaatta ttctcccttg tccccatctc tccctcatcc
    14281 tgtttttaac cttaaaatgg cattattgag gtagacctac atgaaaggac aagaaactcg
    14341 tggttttcag tcagaagtga aacagcttct gcacctgatg atccattctc tctattccaa
    14401 taaagaaatc ttcctgcgtg agcttatctc taacgcctcc gatgcggcgg acaagctgcg
    14461 tttccgcgcg ctctctaacc cggacctgta cgaaggtgat ggcgaactgc gcgttcgtgt
    14521 ctctttcgat aaagacaagc gtacgctgac catctccgat aacggcgtgg ggatgacccg
    14581 cgacgaagtg attgaccatc tggggactat cgctaaatcc ggcactaaat cattccttga
    14641 atcgctgggt tctgaccagg cgaaagacag tcagctgatc ggtcagtttg gtgttggttt
    14701 ctactctgcg tttatcgtgg ccgacaaagt gaccgtgcgt actcgcgcgg caggcgaaaa
    14761 accagaaaac ggcgtcttct gggaatcggc tggcgaaggt gaatataccg ttgccgacat
    14821 caccaaagaa gatcgtggta ctgaaatcac cctgcatctg cgtgaaggcg aagacgagtt
    14881 cctcgatgac tggcgcgtgc gttccatcat cagcaaatac tccgaccata tcgcgctgcc
    14941 ggtagagatc gaaaaacgcg aagagaaaga cggcgaaacc gttatctcct gggagaaaat
    15001 taacaaagcg caggcgctgt ggactcgtaa caagtcggaa atcaccgatg aagagtacaa
    15061 agagttctac aaacacatcg cccacgactt taacgatccg ctgacctgga gccacaaccg
    15121 cgttgaaggt aagcaggagt acaccagcct gctgtacatc ccgtcccagg ctccgtggga
    15181 tatgtggaac cgtgatcata aacacggcct gaaactgtat gttcagcgtg tgttcatcat
    15241 ggacgacgca gaacagttca tgccgaacta tctgcgcttc gtgcgtggtc tgattgactc
    15301 cagcgatctg ccgctgaacg tttcccgtga aatcctccag gacagcacgg taacgcgtaa
    15361 cctgcgcaat gcgctgacca agcgtgtgct gcaaatgctg gaaaaactgg cgaaagacga
    15421 cgcggaaaaa taccagacct tctggcaaca gtttggcctg gtactgaaag aaggtccggc
    15481 ggaagatttc gctaaccagg aagcgatcgc caaactgctg cgttttgctt ctactcatac
    15541 cgattcttct gcgcagaccg tgtctctgga agactacgtt tcccgcatga aagaagggca
    15601 ggagaaaatc tactacatca ctgcagacag ctatgcggca gcgaagagca gcccgcacct
    15661 ggaactgctg cgtaagaaag gcatcgaagt tctgctgctt tccgaccgca tcgatgagtg
    15721 gatgatgaac tatctgactg agttcgacgg taaaccgttc cagtcggtgt ctaaagttga
    15781 cgagtcgctg gaaaaactgg ctgacgaagt tgatgagagc gcgaaagaag cggagaaagc
    15841 actgactccg ttcatcgacc gtgtgaaagc cctgctcggc gagcgcgtga aagatgtccg
    15901 tctgactcac cgtctgaccg atacgccagc gattgtctct actgatgcgg acgaaatgag
    15961 cactcagatg gcgaaactgt tcgctgcggc gggccagaaa gtgccagaag tgaaatacat
    16021 cttcgaactg aacccggatc acgtactggt gaaacgtgcg gcagatactg aagatgaagc
    16081 gaagttcagc gagtgggtag aactgctgct ggatcaggca ctgctggcag aacgcggcac
    16141 gctggaagat ccgaacctgt ttattcgtcg tatgaaccag ctgctggttt cctgatgtaa
    16201 tgccggatga ccttcgtgtc atccggcatt tttcttttca ttatctgcac tttccgcaaa
    16261 ttatctcgcc attaaccgtt tcagcccctg gtgcctttct tgaggcaatc gcctgatggt
    16321 ggtatcgttt atcgcttttt caaaaaattc gacacatttt aaggggattt tcgcaatgcg
    16381 tatcattctg cttggcgctc cgggcgcggg gaaagggact caggctcagt tcatcatgga
    16441 gaaatatggt attccgcaaa tctccactgg cgatatgctg cgtgctgcgg tcaaatctgg
    16501 ctccgagctg ggtaaacaag caaaagacat tatggatgct ggcaaactgg tcaccgacga
    16561 actggtgatc gcgctggtta aagagcgcat tgctcaggaa gactgccgta atggtttcct
    16621 gttggacggc ttcccgcgta ccattccgca ggcagacgcg atgaaagaag cgggcatcaa
    16681 tgttgattac gttctggaat tcgacgtacc ggacgaactg atcgttgacc gtatcgtcgg
    16741 tcgccgcgtt cacgcgccgt ctggtcgtgt ttatcacgtt aaattcaatc cgccgaaagt
    16801 agaaggtaaa gacgacgtta ccggtgaaga actgactacc cgtaaagacg atcaggaaga
    16861 aaccgtacgt aaacgtctgg ttgaatacca tcagatgaca gcaccgctga tcggctacta
    16921 ctccaaagaa gcggaagcgg gtaataccaa atacgcgaaa gttgacggca ccaagccggt
    16981 tgctgaagtt cgcgctgatc tggaaaaaat cctcggctaa tcacgccgca gcagatctgc
    17041 tttcgggcag gtctgctcat ccacctcagg caatacctct cacagcaatt agttcttctt
    17101 cctcactttt ccgctacaat tatcaacaag ttgaatcgat aagaggcggt aatgcgtcag
    17161 actaaaaccg gtatcctgct ggcaaacctg ggtacgcccg atgcccccac acctgaagcg
    17221 gtaaaacgct atctgaaaca atttttaagc gacagacgcg tggttgatac ctcacggttg
    17281 ttatggtggc cattgctgcg cggcgtgatt ttgccgctgc gctcgccgcg tgtggcgaag
    17341 ctgtatgcct ctgtctggat ggaaggtggc tcgccgctga tggtttacag ccgccagcaa
    17401 caacaggcgc tggcacaacg tttaccggag acgcccgtag cgctgggaat gagctacggc
    17461 tcgccatcac tggaaagcgc cgtagatgaa ctcctggcag agcatgtaga tcatattgtg
    17521 gtgctgccgc tttatccgca attctcctgt tctacggtcg gtgcggtatg ggatgaactg
    17581 gcacgcattc tggcgcgcaa acgtagcatt ccggggatct cgtttatacg tgattacgcc
    17641 gataaccacg attacattaa tgcactggcg aacagcgtac gcgcttcttt tgccaaacat
    17701 ggcgaaccgg atctgctgct gctctcttat catggcattc cccagcgtta tgcagatgaa
    17761 ggcgatgatt acccgcaacg ttgccgcaca acgactcgcg aactggcttc cgcactgggg
    17821 atggcaccgg aaaaagtgat gatgaccttt cagtcgcgct ttggtcggga accctggctg
    17881 atgccttata ccgacgaaac gctgaaaatg ctcggagaaa aaggcgtagg tcatatacag
    17941 gtgatgtgcc cgggctttgc tgcggattgt ctggagacgc tggaagagat tgccgagcaa
    18001 aaccgtgagg tcttcctcgg tgccggcggg aaaaaatatg aatatattcc ggcgcttaat
    18061 gccacgccgg aacatatcga aatgatggct aatcttgttg ccgcgtatcg ctaaagctga
    18121 gcggtaaaga actgagcgcc gtcgcgaaga gcctcgtcgg cggttttcat catccgtgaa
    18181 taatgcaaaa aggcgtgcag cgtgcctggg tagagtttga actcacaggg ctgctgatgc
    18241 gccgctaacg tctggtaaag cagacggctg tcatccagca gcggatcgaa ctccgccccg
    18301 gcaataaaac agggcggaac ttcgcgagtg agatcattat taaacagaca gtaatacggc
    18361 gactcgcggt ccgcgtagtt gcttaaatac gcctcttcat acatctgcaa atcctgttgc
    18421 gttaagccat cccagacacc gcccaacaga cgacgagtca cggaatcccg taatccgtaa
    18481 agcccatacc acagcaaaac acccgcaatt ttaccgcaat cgatctgttt atcacgcaac
    18541 cacaacgcac tggcgagcgc cagcatggca cctgcggaat caccggcaaa gccaatgcgg
    18601 gacatattga tttgataatc ctccgcctgc tggtggaaat aacaacaggc agccacaatt
    18661 tcctctatcg cttgcggaaa acgcgcttca ggtgaaaggg tgtaatcaat gccaatcacc
    18721 gtacattggc tgtagcttgc cagcaggcgc atgatgcgat catgggtatc gagattgccg
    18781 agaataaaac cgcctccatg caaataaaat agcgttgccg ggctatctgg ctgcggacaa
    18841 aagagacgtg tttccacctg cccatatttt gttggaacca tgtaagctct ggttgccatt
    18901 tctggagcgc ccgcattcca gaatcggcgc tcaagcgtgt aatactgtcg ttgctcagca
    18961 atcgttcccg ttgcgggcca gggcggtaaa tccggctgaa gagtattcac aacgatcttc
    19021 atttcagcag aaataaggtc cagaacaggt aatttgtttt ccggcttcat acaaaaactc
    19081 ctttcaaatt acgtcattgt aaggaaccac tgccatgaaa atgcgatccc gcctgctgat
    19141 attgaaactg gctgcgtctc gcgcgctccc gtcagattgt gttaacattc gccgctcagt
    19201 taaccacccg taaaaacaac catgaaattt cccggtaaac gtaaatccaa acattacttc
    19261 cccgtaaatg cacgcgatcc gctgcttcag caattccagc cagaaaacga aaccagcgcc
    19321 gcctgggtag tgggtatcga tcaaacgctg gtcgatattg aagcgaaagt ggatgatgaa
    19381 ttcattgagc gttatggatt aagcgccggg cattcactgg tgattgagga tgatgtagcc
    19441 gaagcgcttt atcaggaact aaaacagaaa aacctgatta cccatcagtt tgcgggtggc
    19501 accattggta acaccatgca taactactcg gtgctcgcgg acgaccgttc ggtgctgctg
    19561 ggcgtcatgt gcagcaatat tgaaattggc agctatgcct atcgttacct gtgtaacacc
    19621 tccagccgta ccgatcttaa ctatctacaa ggcgtggatg gcccgattgg tcgttgcttt
    19681 acgctgattg gcgagtccgg ggaacgtacc tttgctatca gccctggcca catgaaccag
    19741 ctgcgggctg aaagtattcc ggaagatgtg attgccggag cctcggctct ggttctcacc
    19801 tcttatctgg tgcgttgcaa gccgggtgaa cccatgccgg aagcaaccat gaaagccatt
    19861 gagtacgcga agaaatataa cgtaccggtg gtgctgacgc tgggaactaa gtttgtcatt
    19921 gccgagaatc cacagtggtg gcagcaattc ctcaaagacc acgtctctat ccttgcgatg
    19981 aacgaagatg aagccgaagc gttgaccgga gaaagcgatc cgttgttggc atctgacaag
    20041 gcgctggact gggtagatct ggtgctgtgc accgccgggc caatcggctt gtatatggcg
    20101 ggctttaccg aagacgaagc gaaacgtaaa acccagcatc cgctgctgcc gggcgctata
    20161 gcggaattca accagtatga gtttagccgc gccatgcgcc acaaggattg ccagaatccg
    20221 ctgcgcgtct attcgcacat tgcgccgtac atgggcgggc cggaaaaaat catgaacacc
    20281 aacggagcag gggatggcgc actggcagcg ttgctgcatg acattaccgc caacagctac
    20341 caccgtagca acgtaccaaa ctccagcaaa cataaattca cctggttaac ttattcatcg
    20401 ttagcgcagg tgtgtaaata tgctaaccgt gtgagctatc aggtactgaa ccagcattca
    20461 cctcgtttaa cgcgcggctt gccggagcgt gaagacagcc tggaagagtc ttactgggat
    20521 cgttaagtta tcgtcggttc gtaggccaga taaggcgttc acgccgcatc tggcattttg
    20581 ctctcaatgc ctgatgcgac gctggcgcgt cttatcaggc ctacatatcc gcgcctatta
    20641 tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt ccagcagttc
    20701 cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc cacgttcggt
    20761 gatatacgcc acttcatcgt cataatgggc gcgggcaata atctcaatat ccggattttt
    20821 cgcgcgggca gatgccacaa tctcgcctgc ttcataaccg ttgggaatcg tcaggatcag
    20881 ccattttgca cattccagat gcgccagttg cataatttct tcgttcgccg cattgcccaa
    20941 tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg tctcaatcac
    21001 caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta cacgaccgta
    21061 accaaccagt agcgcatggt tgcaaatatc cactgggatc tgcttctctt cttcgattgc
    21121 ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct ccagcagcgc
    21181 aaacaggacc gggttgagca taatcgacaa aatcgcacct gccagtacca ggttctgccc
    21241 ggcttgcggc aacagatcca gcgccatgcc cagtcccgcc aggataaacg cgaactcacc
    21301 aatttgcgcc aggctggcgg cgatagttaa tgccgtacgt tgggagtgac caaacagtcg
    21361 caccaggaaa aatgcggcta acgacttacc aaacagaata atcgccagcg tcgccagcac
    21421 tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg agacaaaaaa
    21481 cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt gactcagttc
    21541 agactcgttc agtaccatcc cggcaaagaa cgcaccgagt gcaaaggaga catcaaacag
    21601 ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg taaacagctc
    21661 gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc gacctaccag
    21721 catcataatg gcgataaatg cgatcacctt gccgatggtg atccccatat cgactgcaag
    21781 agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca gcaacaccag
    21841 cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt gcccacgctg
    21901 actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac tggcggtgga
    21961 aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg cagagagcgc
    22021 catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg cgatggcctt
    22081 taccgccatc aaatccttca gcgaaaagtg cagacccacg ccaaacatca acagaatgac
    22141 gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg gagtgaatgg
    22201 tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta gtttattggc
    22261 cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga taagcggggt
    22321 ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg ccgggaaaac
    22381 caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa taattattga
    22441 attttgcaga aaaatggaat tagctgcaaa aaaagcacga atcggaaaac agaaggcggt
    22501 ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt gcctttggct
    22561 gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca tccccaatag
    22621 tggcaggaaa gcacagattt tatagactaa ctcgatgctg gtgtgatcgg cgataagccc
    22681 cagaactgcc gctcccagac ctcccatgcc aaaagcaaaa ccgaaaaaga gtccagaaac
    22741 cataccgata cgtcccggaa gcagctcctg agcgtaaacc agaatggcag agaatgccga
    22801 agcgaggata aatccaataa tcaccgttaa aacccccgtc cagtgcaggc tggcgtaggg
    22861 taaaatcagc gtaaacggcg caacgccgag gatagagccc caaatcacat atttccgccc
    22921 aattttatcc cctacaggcc cgccgatcac cgtacctgcc gcaacggcaa acaggaaggc
    22981 aaacagatga agctgggcat tctggataga taatccgaat ttttgcatca gataaaaggt
    23041 gtaatagctg ctgatgctcg ccatatagaa atatttcgag aaaatgagga ttaacagaat
    23101 gctgaccgcc agtaccactt tgttgcgcgg cagtggattg ataatcgtcg ctttgggttt
    23161 tcctttattc attcggtgct gtgccgagta ccaacggctg atttgcgcca acaccacgat
    23221 cgccagcagt gccgcaagca caaaccaggc aacgttgcct ttgccataag gcgcgataat
    23281 caccgccgcc agcaagggtc ccagggaact gccaaagttg ccgccgacct gaaagataga
    23341 ttgtgccagg ccatgccgcc cgccggaagc catacgggcc acgcgagaag attccggatg
    23401 aaagaccgat gaaccggtac cgaccagcgc cgccgccagc agaactgcgc caaaactgcc
    23461 cgccagcgca agcagcacca gaccacttaa ggtaaagcac atgccaattg gcaacgacca
    23521 cggcatcgga tatttatcgg tccagtagcc gaccactggt tgcagtagcg aagaggcgag
    23581 ctggaaggtg agggttatca tgccaatctg cataaatgtc agagaaaact ctgactgaag
    23641 cagcggataa atcgccagaa tcagcgattg gatcatgtcg ttcagcagat gtgagaggct
    23701 gatagcacct aaaataccaa acgatgttcg ggccttggtc gttgacgcag ccgcgcccgc
    23761 cacaggctgg gtttgttcac tcattgccat aggaaagtca ctttttcagg gttgcgatgt
    23821 aaagaatgat cttatttgtg attattaccc gactaacata cctgtatgcg ccgcctgaag
    23881 gaagtctcaa cgctgaatac agaatttcta atctggatgc agatttatct tcaccggacg
    23941 cagacttgtc tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc aggtcaggga
    24001 gagaagtatg aaattattgc agcggggcgt ggcgttagca ctgttaacca catttacact
    24061 ggcgagtgaa gctgctctgg cgtatgagca ggataaaacc tacaaaatta cagttctgca
    24121 taccaatgat catcatgggc atttttggcg caatgaatat ggcgaatatg gtctggcggc
    24181 gcaaaaaacg ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct
    24241 gctactttcc ggtggcgaca ttaacactgg cgtgcccgag tctgacttac aggatgccga
    24301 acctgatttt cgcggtatga atctggtggg ctatgacgcg atggcgatcg gtaatcatga
    24361 atttgataat ccgctcaccg tattacgtca gcaggaaaag tgggccaagt tcccgttgct
    24421 ttccgcgaat atctaccaga aaagtaccgg cgagcgcctg tttaaaccat gggcgctgtt
    24481 taagcgtcag gatctgaaaa ttgccgttat tggtctgacg acggatgaca cagcgaagtt
    24541 aggcaaccca gaaaacttca ccgatattga gttccgtaag cccgccgatg aagcgaagct
    24601 ggtgattcag gagctgcaac agacagaaaa gccagacatt attatcgcgg cgacccatat
    24661 ggggcattac gacaatggtg agcacggctc taatgcaccg ggcgatgtgg agatggcacg
    24721 cgtgctgcct gccggatcgc tggcgatgat cgtcggtggt cactcgcaag atccggtctg
    24781 catggcggca gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat gcaaaccgga
    24841 tcaacaaaac ggcatctgga ttgtgcaggc acatgagtgg ggcaaatacg tgggacgggc
    24901 tgattttgag tttcgtaatg gcgaaatgaa aatggttaac taccagctga ttccggtgaa
    24961 cctgaagaag aaagtgacct gggaagacgg gaaaagcgag cgcgtgctgt acacccctga
    25021 aatcgctgaa aaccagcaaa tgatctcgct gttatcgccg ttccagaaca aaggcaaagc
    25081 gcagctggaa gtgaaaatag gcgaaaccaa tggtcgtctg gaaggcgatc gtgacaaagt
    25141 gcgttttgta cagaccaata tggggcggtt aattctggca gcacaattgg atcgcactgg
    25201 tgccgacttt gcggtgatga gcggaggcgg aattcgtgat tctatcgaag caggcgatat
    25261 cagctataaa aacgtgctga aagtgcagcc attcggcaat gtggtggtgt atgccgacat
    25321 gaccggtaaa gaggtgattg attacctgac cgccgtcgcg cagatgaagc cagattcagg
    25381 tgcctacccg caatttgcca acgttagctt tgtggcgaaa gacggcaaac tgaacgacct
    25441 taaaatcaaa ggcgaaccgg tcgatccagc gaaaacttac cgtatggcga cattaaactt
    25501 caatgccacc ggcggtgatg gatatccgcg ccttgataac aaaccgggct atgtgaatac
    25561 cggctttatt gatgccgaag tgctgaaagc gtatatccag aaaagctcgc cgctggatgt
    25621 gagtgtttat gaaccgaaag gtgaggtgag ctggcagtaa tccgaaagtg ccggatgttc
    25681 gcatccggca caatgcttaa tcgcggcggg cgatatcagc aaatttggca tcgaggatct
    25741 tcgccagatc gcctgccgcc agttcgatat ccagtccgcg cttgccgccg gaaacataaa
    25801 tagtggcaaa ttcttgtgcg ggggcgtcga taatcgttgg cagacgtttt ttctgcccca
    25861 gtgggctaat ccccccaacc aggtatcccg tcgaacgctg cgcgaccatc ggatcggcca
    25921 tctcaacttt cttggcaccc agcgcttttg ctactttttt aaggtccagt tgacctgcga
    25981 ccggcgtaac ggccacggca aggtgtttca tatcaccgtt cactgccacc agcagcgttt
    26041 tgtagacctg atccggattc aaacctaatt ttttgacgac ttcatcgcca aaattggttt
    26101 cagccggatc gtgctcgtag gtatggattt gaaacgaaat cttgtttttt tcgagtaatt
    26161 taactgcggg tgtcatggta attttcctgc cttaaacaaa aaaacgaagc aagcatacgc
    26221 ctgatggcgg tccaaaaaaa agagtcatct tgcctaagag tattggcagg atggtgagat
    26281 tgagcgacaa tcgagttaca ccgtcgctta aagtgacggc ataataataa aaaaatgaaa
    26341 ttcctctttg acgggccaat agcgatattg gccatttttt tagcgcaaca tttgcggtaa
    26401 attcccttct ccatacaggt gtagtgcacc gaccgcgacc acatatcgcc ccggcggcat
    26461 ggcgcgtaat ttatcccgcc aggcgagatt tcgctgatgc atcagcacat cgtacagcga
    26521 ctgactgaac gtattgggca gcgttatttc attattttgc ggcggcgcat tcagccacca
    26581 gctcatcatt tgttgcagca accgtgcatt ggtgtgccag tgggtcagcg tatcgtccag
    26641 cagtgccagt cctttgtcag ggagctggag caacatggca atctggtttt cagccccttc
    26701 cagttcaatc acgggtttat gttgttgctt cgccgcctgc aatagctggt aatcgatacc
    26761 gtattctgcc cgcagcccca gtttttgcgc ctgcgtcgcc tgaagaacca tcgcgatttg
    26821 ccacagcggt tgggtagaaa agagtgaggg agaaatgccc atctcctggc taatgtgctg
    26881 cagtttttgg agttgctcct cgctaatgcg ctcttccagc gcctcgcagg caggcaaatt
    26941 agcaaaaggc gtatcgttgg tggaaacatc cgcctcgacg atcagcgcat cggcgttttt
    27001 gagctttttg agcaaacggg tgggcagggg agccatatcg tggctaccca tatgaatact
    27061 gccaatcaga tgaaaatggc gattgccggg aagggtgata tcgatggcag gccaggtgta
    27121 atgattaccg cgcagcgcgg cccaaagcgt ttttacccgg tacaacagat ccatacgacc
    27181 tccctttgtg aaatatcatg ctagcgcgcg gtgagggatg gcgcaaatgg agttggggac
    27241 ttgtttaatg tgtttgtatg attcagtatg ttcttgtatc gctattcaca gggaagcaac
    27301 agttaaaaac tatgaaacag gcaacaagaa aaccgacgac acccggagat attcttctct
    27361 atgaatatct ggaaccgctc gctttgaaaa tcaatgagtt agcagagttg ctgcatgttc
    27421 atcgtaatag cgtcagtaca ctgatcaata ataatcgtaa actcaccact gaaatggcat
    27481 tccgtctggc gaaagttttt gataccacag tcgatttttg gctaaacctc caggcggcag
    27541 ttgatctttg ggaagttgaa aacaacatgc gtacccagga agaattggga cggattgaaa
    27601 cagtggctga atatttggca cgccgtgaag agcgtgcaaa aaaggtcgcg taaggccaaa
    27661 acatcgcttc atctcgtagg ccggataagg cgtttacgcc gcatccggca tccgagcacc
    27721 attgcctgat gcgacgctta cgcgtcttat caggcctaca aacctgtgcc tgaaccgtag
    27781 gccggataag gcgttcacgc cgcatccggc agtcatgcgt cgatgccaaa tgcgccgccc
    27841 taaagcagcg catccgcaat gacgtactta ttccttcggt ttaaaccgca gcaaccggtt
    27901 ggcgttactc actacggtaa tcgacgagag cgccattgcc gctccggcaa ctaccgggtt
    27961 aagcagtgtt ccagtaaacg gccacaaaat accggcggcg accggaatac cgatactgtt
    28021 gtagataaac gcgccgagca ggttctgttt catgttgcgc agcgttgcgc gggaaatagc
    28081 gagcgcatcc gcaacgccca tcaggctatg gcgcatcagg gtaatcgccg cggtttcaat
    28141 ggcaacatca ctgccgcccc ccatcgcaat gccgacatcc gcctgggcca gcgctggcgc
    28201 gtcgttaatg ccgtcgccca ccattgccac ctgacgtcct tcactttgca gacgtttgat
    28261 cgcttcggct ttaccgtccg gcagcacccc ggctatcacc tcatcaatcc cggcttcttt
    28321 ggcgatcgca ttggcggtgg ttgggttatc cccggtcaac atcaccagac gatatcccgc
    28381 tttatgcagg cgttgcagtg ccgccacgct atcactacgc aatggatcgc gtacagccag
    28441 cagggctact gctttcccgt caatcgccag cagcacaggc gttgctcctt gcgatgcctg
    28501 agctgaaata tcggcttcaa tcgctttagt atcgacttgc tgatcattta acaacgcctg
    28561 attacccagc aataacgcat gaccttctgc ttcaccgctc acacccagcc cgcgcaatgt
    28621 gcggaaaccg ttgacctgcg gcagctgcat atcgcttgct ttatcgagga tcgcccgtgc
    28681 cagcggatgg ctggaacctt gttccagtgc cgccgccaga cgcagcgcct gcgcttcgtc
    28741 agaatcagca aatgttttca ctgcgacaac ctgcggcttc ccttcggtca gcgtcccggt
    28801 tttatcgaac acgacggtat cgagtgtact ggcgcgttgc agcgcgtcgg cgtcccgcac
    28861 cagcacgcca aactcagccg cccgcccgac gccggaaata atcgacatcg gcgtcgccag
    28921 ccccagcgca cacggacagg caataatcag caccgtggtg gcaatcacca gggtatagac
    28981 aatctgcggt gccggaccaa agaaatacca gattgccgca ctgacaagcg caataaccac
    29041 tactaccggc acaaatacgg ctgagatttt atccgccagc tgaccgattt ctggcttgct
    29101 gctctgggcc tggcgcacca tacgaataat ccttgatagc gtggtatggc tgccaaccgc
    29161 actggcgcga aacagaacgc taccgtcctg taccactgtc ccggcatgga cgctatcgcc
    29221 ttcgcctttt tgctgcggga tcggttcgcc cgtcagcatc gcttcatcca gccacgcttc
    29281 gccctgggta atctcgccat ctaccggcac gcggtcgccg gtcgtcaggc gcagtagcat
    29341 gcctggctga acttccgcca gcggcacgct tttttcacct tcgtcggtga ccaggcgcgc
    29401 cgtcggcgga gttaaatcga gtaatttctc cagcgccttt gaagaacgct gacgtgcgcg
    29461 tgcttccagc atatggccga gattgatcag accgataatc atcgcgctgg cttcgtaata
    29521 aagatgtcgc gcttccatcg ggaaccactg cggccacagg ttgacgctca tcgaatagag
    29581 ccacgccacg ccagtaccca gcgccaccag cgtatccatc gtcgctgcac cgttcagcag
    29641 gcttttccat gcactgcggt aaaaatggcc gccggcgaaa accatcactg ccagggttat
    29701 caggccgata actaaccaca agctgcggtt gtcagcggtg accatcatgt tatcgccgat
    29761 catcccccag accattaccg ggatacccac agccagtgca acaattgcct gccagcggaa
    29821 gcgcttcatc gtagcgacgg cggtttcttg ctggcgctcg cggcgtttcg cgtcatcttc
    29881 aatcgcttcc gcaccgtagc ccgctttttc caccgcctgc actaaatctt gtggggaggc
    29941 actgcccatc accagcgcag tacgctccgc caggtttacc cgtgcctgag tgacgcccgg
    30001 tacgctttgc aacgcatttt gtacgcgggt gacacagctg gcgcagctca tgccgctcag
    30061 cagcaactgc tggctgtcat cgtcatcggc ggtcgctgcc ggaagcgcct cagaaaccgc
    30121 tgtcagtgct tccgacggga ttgatgactc cgccagcggt ttagcctttg ggtggcttac
    30181 agatgcgtca taacccgctt gtttgatggt ttcgatcagc tgttctgcac tggcagtccc
    30241 ggtaacgtgc gcttcagtga tagacacatc cgcttgttcc acatccgggc gctgttcgag
    30301 actttctttc acgcgtttaa cgcagtgacc gcaggacagg ccgtccaggg tcaggtcgat
    30361 agtttgtgac ataaaacact cctttaagac agttttgact ggctgtgata aaggttaaac
    30421 cttccagcaa ggggaaggtc aagaaattaa taacccaggc gagtaaaagt ccgtaaagat
    30481 taaaaaatcg gctcgatttg catcaggatt agacatttat ctctttgttt tcctgtagtt
    30541 aagttgcggg tgctaagtta aatcaggatg cctgaaaatc ggcaccgggg tgaggaatta
    30601 cctcccgcat ctataaaaag gagttaacaa aagatgttag atgcaaacaa attacagcag
    30661 gcagtggatc aggcttacac ccaatttcac tcacttaacg gcggacaaaa tgccgattac
    30721 attccctttc tggcgaatgt gcctggtcaa ctggcagcag tggctatcgt gaccagcgat
    30781 ggcaacgtct atagtgcggg tgacagtgat taccgctttg cgctggaatc catctcgaaa
    30841 gtctgtacgt tagcccttgc gttagaagat gtcggcccgc aggcggtaca ggacaaaatt
    30901 ggcgctgacc cgaccggatt gccctttaac tcagttatcg ccttagagtt gcatggcggc
    30961 aaaccgctgt cgccactggt aaatgctggc gctattgcca ccaccagcct gattaacgct
    31021 gaaaatgccg aacaacgctg gcagcgaatt ttacatatcc aacagcaact ggctggtgag
    31081 ctggtagcgc tctctgacga agtcaaccag tcggaacaaa caaccaactt ccataaccgg
    31141 gccattgcct ggctgctgta ctccgccgga tatctctatt gtgatgcaat ggaagcatgt
    31201 gacgtgtaca cccgtcagtg ctccacgctc atcaatactg ttgaactggc aacgcttggc
    31261 gcgacgctgg cggcgggtgg tgtgaatccg ttgacgcata aacgcgttct tcaggccgac
    31321 aacgtgccgt acattctggc cgaaatgatg atggaagggc tgtatggtcg ctccggtgac
    31381 tgggcgtatc gtgttggttt accgggcaaa agcggtgtag gtggcggtat tctggcggtc
    31441 gtccctggag tgatgggaat tgccgcgttc tcaccaccgc tggacgaaga aggcaacagt
    31501 gttcgcggtc aaaaaatggt ggcatcggtc gctaagcaac tcggctataa cgtgtttaag
    31561 ggctgatcat gatgaacacg gaaggtaata acggtaacaa acccctcggt ctatggaacg
    31621 tcgtttccat
//
LOCUS       DAZGBC010000040        31561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGBC010000040 DAZGBC010000000
VERSION     DAZGBC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.040"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..183
                     /locus_tag="ACHK1J_001866"
     CDS             <1..183
                     /locus_tag="ACHK1J_001866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911142.1"
                     /translation="PTRFTSDCNYVLYETQEEITIYRDSSRRKKNNNIHETHPDGSIV
                     NSPITGIPPAFYGCFH"
     gene            327..1670
                     /locus_tag="ACHK1J_001867"
     CDS             327..1670
                     /locus_tag="ACHK1J_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911143.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1819..3327)
                     /gene="putP"
                     /locus_tag="ACHK1J_001868"
     CDS             complement(1819..3327)
                     /gene="putP"
                     /locus_tag="ACHK1J_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO7911144.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3486..3695)
                     /locus_tag="ACHK1J_001869"
     CDS             complement(3486..3695)
                     /locus_tag="ACHK1J_001869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911145.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3750..7712
                     /gene="putA"
                     /locus_tag="ACHK1J_001870"
     CDS             3750..7712
                     /gene="putA"
                     /locus_tag="ACHK1J_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7911146.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQTVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVELALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENNEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7752..8390)
                     /gene="rutR"
                     /locus_tag="ACHK1J_001871"
     CDS             complement(7752..8390)
                     /gene="rutR"
                     /locus_tag="ACHK1J_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO7911147.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8678..9769
                     /gene="rutA"
                     /locus_tag="ACHK1J_001872"
     CDS             8678..9769
                     /gene="rutA"
                     /locus_tag="ACHK1J_001872"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO7911148.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGYFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9769..10461
                     /gene="rutB"
                     /locus_tag="ACHK1J_001873"
     CDS             9769..10461
                     /gene="rutB"
                     /locus_tag="ACHK1J_001873"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO7911149.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10473..10859
                     /gene="rutC"
                     /locus_tag="ACHK1J_001874"
     CDS             10473..10859
                     /gene="rutC"
                     /locus_tag="ACHK1J_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO7911150.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10867..11667
                     /gene="rutD"
                     /locus_tag="ACHK1J_001875"
     CDS             10867..11667
                     /gene="rutD"
                     /locus_tag="ACHK1J_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO7911151.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11677..12267
                     /gene="rutE"
                     /locus_tag="ACHK1J_001876"
     CDS             11677..12267
                     /gene="rutE"
                     /locus_tag="ACHK1J_001876"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO7911152.1"
                     /translation="MNEAVSPGALSTLFTNARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12278..12772
                     /gene="rutF"
                     /locus_tag="ACHK1J_001877"
     CDS             12278..12772
                     /gene="rutF"
                     /locus_tag="ACHK1J_001877"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO7911153.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            12793..14121
                     /gene="rutG"
                     /locus_tag="ACHK1J_001878"
     CDS             12793..14121
                     /gene="rutG"
                     /locus_tag="ACHK1J_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO7911154.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14204..14377)
                     /gene="ymdF"
                     /locus_tag="ACHK1J_001879"
     CDS             complement(14204..14377)
                     /gene="ymdF"
                     /locus_tag="ACHK1J_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7911155.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            14750..15346
                     /gene="wrbA"
                     /locus_tag="ACHK1J_001880"
     CDS             14750..15346
                     /gene="wrbA"
                     /locus_tag="ACHK1J_001880"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7911156.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15367..15594
                     /gene="yccJ"
                     /locus_tag="ACHK1J_001881"
     CDS             15367..15594
                     /gene="yccJ"
                     /locus_tag="ACHK1J_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO7911157.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15632..16873)
                     /gene="agp"
                     /locus_tag="ACHK1J_001882"
     CDS             complement(15632..16873)
                     /gene="agp"
                     /locus_tag="ACHK1J_001882"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO7911158.1"
                     /translation="MNKTLITATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(17166..18419)
                     /locus_tag="ACHK1J_001883"
     CDS             complement(17166..18419)
                     /locus_tag="ACHK1J_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFO7911159.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEKNKTYSITLFENTLVCF
                     MRLYKEIRRQQAEDYIPCLECDSLEKEFEEIQNDNDLSLFMKTLRTNDKQIYSGVSGG
                     ITYTIQYVEDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLDD
                     ENISLQPEWINAPGISDLDTGIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAILV
                     LKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSD
                     NKIQGEGSLMSWNFLFFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEKRIN
                     EVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPPE
                     LFAQAYKLNNKEMNAQLDYLNRYAI"
     gene            18679..19599
                     /gene="cbpA"
                     /locus_tag="ACHK1J_001884"
     CDS             18679..19599
                     /gene="cbpA"
                     /locus_tag="ACHK1J_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO7911160.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            19599..19904
                     /gene="cbpM"
                     /locus_tag="ACHK1J_001885"
     CDS             19599..19904
                     /gene="cbpM"
                     /locus_tag="ACHK1J_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO7911161.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(19997..20596)
                     /gene="torD"
                     /locus_tag="ACHK1J_001886"
     CDS             complement(19997..20596)
                     /gene="torD"
                     /locus_tag="ACHK1J_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO7911162.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAMDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(20593..23139)
                     /gene="torA"
                     /locus_tag="ACHK1J_001887"
     CDS             complement(20593..23139)
                     /gene="torA"
                     /locus_tag="ACHK1J_001887"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO7911163.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSKNLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTIAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVDQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(23139..24311)
                     /gene="torC"
                     /locus_tag="ACHK1J_001888"
     CDS             complement(23139..24311)
                     /gene="torC"
                     /locus_tag="ACHK1J_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO7911164.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDKLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            24441..25133
                     /gene="torR"
                     /locus_tag="ACHK1J_001889"
     CDS             24441..25133
                     /gene="torR"
                     /locus_tag="ACHK1J_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO7911165.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQVQPLTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(25106..26134)
                     /gene="torT"
                     /locus_tag="ACHK1J_001890"
     CDS             complement(25106..26134)
                     /gene="torT"
                     /locus_tag="ACHK1J_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO7911166.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQSSTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            26217..28961
                     /gene="torS"
                     /locus_tag="ACHK1J_001891"
     CDS             26217..28961
                     /gene="torS"
                     /locus_tag="ACHK1J_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO7911167.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTLEVGSCFCLRLPLRVATAPAPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLEHYL
                     QLQVNNDIPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            29033..30106
                     /gene="yccM"
                     /locus_tag="ACHK1J_001892"
     CDS             29033..30106
                     /gene="yccM"
                     /locus_tag="ACHK1J_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7911168.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRYMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(30154..30327)
                     /gene="gnsA"
                     /locus_tag="ACHK1J_001893"
     CDS             complement(30154..30327)
                     /gene="gnsA"
                     /locus_tag="ACHK1J_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO7911169.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(30317..30547)
                     /gene="ymcE"
                     /locus_tag="ACHK1J_001894"
     CDS             complement(30317..30547)
                     /gene="ymcE"
                     /locus_tag="ACHK1J_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO7911170.1"
                     /translation="MHRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            complement(30522..30710)
                     /gene="ymcF"
                     /locus_tag="ACHK1J_001895"
     CDS             complement(30522..30710)
                     /gene="ymcF"
                     /locus_tag="ACHK1J_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO7911171.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(30721..30933)
                     /gene="cspG"
                     /locus_tag="ACHK1J_001896"
     CDS             complement(30721..30933)
                     /gene="cspG"
                     /locus_tag="ACHK1J_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO7911172.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            31219..31431
                     /gene="cspH"
                     /locus_tag="ACHK1J_001897"
     CDS             31219..31431
                     /gene="cspH"
                     /locus_tag="ACHK1J_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO7911173.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7657 a         8247 c         8045 g         7612 t
ORIGIN      
        1 ccgacaagat tcacgagtga ttgtaactac gtactttatg aaacacagga agaaattact
       61 atttaccgcg atagttcaag gcgtaaaaaa aataataata ttcatgaaac tcatcccgat
      121 ggcagcattg taaattcgcc aataactggc attcctcctg ctttttacgg gtgtttccat
      181 taacaccaga atgagcggat tgtttactac atcaagaacg catccgaagt gtacatcctg
      241 gtgcgccgac tgaatcgccc ggagccatgc ggatggctac gacttaatac gaatcattaa
      301 ccctgtaagg atacggagag catgacatga gaccaatatc caacctcaat acgagcgcag
      361 caacctatat tccaccgcaa caactcccga gtagttgtct ggaaactttg acgcttctgg
      421 tgcagatgaa taattatatc aaaagggatg cggactacag cacgggcatg gctgttactc
      481 cactggttcc tgaagaggtt cacttgctgg ctgttgcgat gacaaccgaa ctacatcgac
      541 atcagttcca accggtggta agtgcaaatg atctgcaact tcctgaaccg ttcaccttcg
      601 atgttgccgg tttcaccatc accttcacga agacgcaaga gcatgacaac acgggcaatc
      661 tcatgaaaat tgcagtcagc aagagcggtg tgtgcaccag tacaaatatt actcttgagc
      721 tgtttcactc cattgtcaca accctgatgt cacgcagtca atatggaacc tttgatctct
      781 ggtccgtacg gccaatactc acggatgaaa gtcaaaacag ggtgcatgaa gcagctcgct
      841 actccccggc ccagcaatac ggaagagaag aaacacactt taccaacaat ggcatgcgag
      901 aatttggaaa tatgtctccg ctggcgtggc gtaacgatgt ggaattacag caatcgtgta
      961 atacaagcaa catcccacca aatgctgcaa atgacgacat aaacaacacc aggcagacca
     1021 tcgaggacca gccagaggcg gacgagccac agcaatatgt taaattgact gtagacgaca
     1081 tgcgaaaatg ggcggctatg gaccagcagg cgcgaaacgc tctacaggga gtttcaggat
     1141 ggtgtacgcg caatcatttt aatatcaaaa atgccagaaa ttacctgacc gatcacggcc
     1201 tgaactacgc gggacaggta aaggtaaaca ggccgcatga atatgccaaa ttcacccttg
     1261 aacatatccg ccagtgggca gccttaaaca agcatgtgcg aaaaccggtt ggttatttag
     1321 aaaagtggtg taaggaaaga aacctcgcgc caactactgc caggaattac ctgaagaacg
     1381 acgggcttac cgccctgggt gagttgaagc tgacagggcc gcagaaatgg gtcacgttca
     1441 ccttcggaga catcgtacaa tgggcgaata tgacccagga ggagcgtaac agcgcagggg
     1501 gagccgaaaa gtggagtaaa aaacgcggct tccagtggtc tactgccaga tcctacctga
     1561 aatcgagcgg agtgaccaag caaggtgcga gaaaactggc gtggttaaaa aacagcggga
     1621 atatgtcgaa tccattctat ctcgcccaac caaccagccg caggacataa gctgaaggta
     1681 ccgatagcgg caattttgtt catcagtgcc gggtgggcaa atagcaattg cccccgaaca
     1741 ctccctgcca ggcaacttat accgcatggc agggaacatt cgggttaaaa agcaaaagat
     1801 aaataattac tcttcagctt agccttcctg caaccgtgac ggcggtgccg aatgatagtg
     1861 cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac ggcgctttac ccagcaaact
     1921 aaacactaca atcccaatac tgccgaagat aaagcccgga ataatttcgt acagtcccag
     1981 ccagccgaac tgtttccaga cgataaccgt cagcgccccg atgatcatcc ccgccagcgc
     2041 accgttgcgc gtcatgcgtg accacatcac cgagaacagc accaccggac caaacgccgc
     2101 gccaaagcct gcccacgcgt agctcactaa gcccagcacg cggttttccg ggtttgccgc
     2161 cagcgcaatg gccaccagcg ccaccaccag caccatcaca cgccctaccc acaccagctc
     2221 tttctggctg gcatgtttac gcagaaacgc tttatataga tcttcggtaa tcgcactgga
     2281 acataccagc agctggcaac ttaaggttga cattaccgcc gccagaattg ccgacagcag
     2341 aatcccggca atccacgggt taaacagaat ttgcgccagt tcgataaaca cgcgttcagc
     2401 gttctggttt accgcacctg ctaccgccgg gtgctcgtta aagtaagcga taccaaagaa
     2461 gccgacagcc accgcccctg ccaggcagag gatcatccag gtcatactga tacgacgcgc
     2521 atggacgatg ctgtggtgag aatccgccgc cataaaacgc gccaggatgt gcggctgccc
     2581 gaagtaaccc agcccccatc ccatcagcga gataatggca acaaagttca gccctttgag
     2641 catatccacg ttttcgatgc tcttttgttt gatcacttcc agagagtcgc caaagccacc
     2701 gacactgata atgacgataa ccggcgtcag gatcagggca aaaatcatca ggctggcctg
     2761 tacagtatca gtccagctca ccgcgaggaa accgccaata aaggtgtaaa ggatcgtcgc
     2821 cgcagctccc gcccacagag ccgtttcgta gctcatgcca aaggtacttt caaacagacg
     2881 cgcgcctgcc acaatgcccg aagcacaata aatggtgaag aacagcaaaa taaccagcgc
     2941 ggagataatg cgcaaaatgc ggcttttatc ttcaaagcgt ccggtgaaat aatccggcaa
     3001 tgttaaggcg ttattgttat attcggtatg cacacgcaac cgcccggcca ccagcttcca
     3061 gttgatccac gcgcctaatg tcaggccaat ggcgatccaa ctttcggaaa tcccggaaag
     3121 aaaaaccgcg cccggcaacc ccattaacag ccagccgctc atatccgatg cacccgccga
     3181 taatgccgtc acgaatggcc caaggctacg tccgcccaga atatagtcgt caaagttttt
     3241 cgttgatcgc caggcgataa acccaatcaa tatcatgcca aagatataga cacaaaatgt
     3301 caccaacatc ggtgtgctaa tagccatcta aagtctccaa aaaattatta tcggcaatgt
     3361 cgaaacttgc cgttatatct gccaccggaa cggggtaaca gagtttatgt tttaccaggg
     3421 cgaccgtatc ctgccggaag cgctagttat tcacaatcga tttaacacac catttacatt
     3481 aaattttagt gctcagcgac actatttttc atcaggttgc actctctcac attttttgcg
     3541 gttgcacctt tcaaaaatgt taactgccgc agagaaaaag tctgagttat ttttttaatc
     3601 cctgtcatat cgatttcttt tattaacatt tcattcattt ttaagcttgc tacgcaagtc
     3661 acatttaaca tggttgcaca aagttgcaac atcatggata tttcacgata acgttaagtt
     3721 gcacctttca gaacaacagg agtaatggca tgggaaccac caccatgggg gttaagctgg
     3781 acgacgcgac gcgtgagcgt attaagtctg ccgcgacacg tatcgatcgc acgccacact
     3841 ggttaattaa gcaggcgatt ttttcttatc tcgaacaact ggaaaacagc gatactctgc
     3901 cagagctacc tgcgctgctt tctggcgctg ccaatgagag cgatgaagca ccgactccgg
     3961 cagaggaacc acaccagcca ttcctcgact ttgccgagca aattttgccc cagtcggttt
     4021 cccgcgccgc gatcaccgcg gcctatcgcc gcccggaaac cgaagcggtt tctatgctgc
     4081 tggaacaagc ccgcctgccg cagccagttg ctgaacaggc acacaaactg gcgtatcaac
     4141 tggccgataa actgcgtaat caaaaaaatg ccagtggccg cgcaggtatg gtccaggggt
     4201 tattgcagga gttttcgctg tcatcgcagg aaggcgtggc gctgatgtgt ctggcagaag
     4261 cgttgttgcg tattcccgac aaagccaccc gcgacgcgtt aattcgcgac aaaatcagca
     4321 acggtaactg gcagtcacac attggtcgca gcccgtccct gtttgttaat gccgccacct
     4381 gggggttgtt gtttactggc aaactggttt ccacccataa cgaagccagc ctctcccgct
     4441 cgctgaaccg cattatcggt aaaagtggtg aaccgctgat ccgcaaaggt gtggatatgg
     4501 cgatgcgcct gatgggcgag cagttcgtca cgggcgaaac catcgcggaa gcgttagcca
     4561 atgcccgcaa gctggaagag aaaggtttcc gttactctta cgatatgctt ggcgaagctg
     4621 cactaactgc cgcagatgca caagcgtata tggtttccta tcagcaggcg attcacgcca
     4681 ttggtaaagc gtctaacggt cgtggcatct atgaaggtcc gggcatttca atcaagctgt
     4741 cggcgctgca tccgcgttac agccgcgccc agtatgaccg ggtaatggaa gagctttacc
     4801 cacgtctgaa atcactcacc ctgctggcgc gtcagtacga tattggtatc aacattgacg
     4861 ccgaagaggc cgatcgtctg gagatctccc tcgatctgct ggaaaaactc tgtttcgagc
     4921 cggaactggc aggctggaac ggcatcggct ttgttattca ggcttatcaa aaacgctgcc
     4981 cactggtgat tgattacctg attgatctcg ccacccgcag ccgtcgccgt ctgatgattc
     5041 gcctggtaaa aggcgcgtac tgggatagtg aaatcaagcg tgcacagatg gacggccttg
     5101 aaggttatcc ggtttatacc cgcaaggtgt ataccgacgt ttcttatctc gcctgtgcga
     5161 aaaagctgct ggcggtgccg aatctaatct acccgcagtt cgcgacgcac aacgcccata
     5221 cgctggcggc gatttatcaa ctggcaggtc aaaactacta tccgggtcag tacgagttcc
     5281 agtgcctgca tggtatgggc gagccactgt atgagcaggt caccggaaaa gttgccgacg
     5341 gcaaacttaa ccgtccgtgt cgtatttatg ccccggttgg cacacatgaa acactgctgg
     5401 cgtatctggt gcgtcgcctg ctggaaaacg gtgctaacac ctcgtttgtt aaccgtattg
     5461 ccgacacctc tttgccattg gatgaactgg tcgccgatcc ggtcactgct gtagaaaaac
     5521 tggcgcaaca ggaagggcaa actggattac cgcatccgaa aattcccctg ccgcgcgatc
     5581 tttatggtca cgggcgcgac aactcggcag ggctggatct cgctaacgaa caccgtctgg
     5641 cctcgctctc ttctgccctg ctcaatagtg cactgcaaaa atggcaggcc ttgccaatgc
     5701 tggaacaaac ggtagcggct ggtgagatgt cgcccgttat taaccctgcg gaaccgaaag
     5761 atattgtggg ctatgtgcgt gaagccacgc cgcgtgaagt agaactggcg ctggaaagtg
     5821 cggttaataa cgcgccaatc tggtttgcca cgcctccggc tgaacgcgcg gcgattttgc
     5881 atcgcgctgc cgtgctgatg gaaagccaga tgcagcaact gattggtatt ctggtgcgtg
     5941 aggccggaaa aaccttcagt aacgccattg ccgaagtgcg tgaagcggtc gattttctcc
     6001 actactacgc cggacaggtg cgggatgatt tcgctaacga aacccatcgt ccattagggc
     6061 ctgtggtatg tatcagcccg tggaacttcc cgctggcgat tttcaccggg cagattgctg
     6121 ccgcactggc ggcaggtaac agcgtgctgg caaaaccggc agaacaaacg ccgctgattg
     6181 ccgcgcaagg gatcgccatt ttgctggaag cgggtgtacc gccaggcgtg gtgcagttgc
     6241 tgccaggtca gggggaaacc gtgggcgcgc aactgacggg tgatgatcgc gtgcgcgggg
     6301 tgatgtttac cggttcaacc gaagtcgcta cgttactgca gcgcaatatc gccagccgcc
     6361 tggacgctca gggtcgccct attccgctca tcgctgaaac cggcggcatg aacgcgatga
     6421 ttgtcgattc ttcagcactg accgaacaag tagtaataga tgtgctggcc tcggcgttcg
     6481 acagtgcggg tcagcgttgt tcggcactgc gcgtgctgtg cctgcaagat gagattgccg
     6541 accacacgtt gaaaatgctg cgcggcgcaa tggccgaatg tcggatgggt aatccgggtc
     6601 gcctgaccac tgatatcggt ccggtgattg atagcgaagc gaaagccaat atcgagcgtc
     6661 atattcagac catgcgtagc aaaggccgtc cggtgttcca ggcggtacgg gaaaacaacg
     6721 aagatgcccg tgaatggcaa agcggcacct ttgtcgcccc gacgctgatc gaactggatg
     6781 actttgccga attgcaaaaa gaggtctttg gtccggtgct gcatgtggtg cgttacaacc
     6841 gtaaccagct gccggagctg atcgagcaga ttaacgcttc cggttatggt ctgacgcttg
     6901 gcgtccacac gcgcattgat gaaaccatcg cccaggtcac tggctcggcc catgtcggta
     6961 acctgtacgt taaccgtaat atggtgggcg cagtggtggg tgtgcagcca ttcggcggcg
     7021 aaggattgtc cggcaccggg ccaaaagcag gcggtccgct ctatctctac cgtctactgg
     7081 cgaatcgccc ggaaagtgcg ctggcagtga cgctcgcgcg tcaggatgca gagtatccgg
     7141 tcgatgcgca gttgaaagcc gcattgactc agccgctaaa tgcactgcgg gaatgggcag
     7201 caaatcgccc agaattgcag gcgttatgta cgcaatatgg cgagctggcg caggcaggaa
     7261 cacaacgatt gctgccaggg ccgacgggtg agcgcaacac ctggacgctg ctgccgcgtg
     7321 agcgcgtatt gtgtatcgcg gatgatgaac aggatgcgct gactcagctc gccgccgtgc
     7381 tggcggtggg cagccaggtg ctgtggccag atgacgcgct gcatcgtcag ttagtgaagg
     7441 cattgccatc gactgtcagc gagcgtattc aactggcgaa agcggaaaat attaccgctc
     7501 aaccgtttga tgcggtgatc ttccacggtg attcggatca gcttcgcgca ttgtgtgaag
     7561 cagttgccgc acgggatggc gcaattgttt cggtgcaggg ttttgcccgt ggcgaaagca
     7621 atatccttct ggaacggctg tatatcgagc gttcgctgag tgtgaatacc gctgccgctg
     7681 gcggtaacgc cagcttaatg acgatcggtt aaacaggtag ccggaggatg ttacaccttc
     7741 ctccggcatc attagcgtgg tcgaatcccc tcaataataa tccgctgcac gttttcaacc
     7801 gtttgattga aaaatacctc atcgcgcaac gttgcgcctg tcaccgcctc cacctgaggg
     7861 gcgaaatcgg cgtaatgttg agtggaagcc caaatcataa aaatcaaatg ctgcggatca
     7921 atcggcgcga gtttgccgct tttgacccaa ccggcaatca gcgccgattt ctcatcaatt
     7981 aatgccttca aatcgcccgt cagttcatcc attaacagcg gcgcgcctgc cagcatctcc
     8041 atacagaaca ggcgcgaagc ctgcggataa tcgcgtgaga cttccagctt cagacggatg
     8101 tactctttga tcgccgccag cggggcgaaa tcttcacgaa acgcttttaa cggtgccagc
     8161 cagatatcga gaatctgccg cagcacggca atatacagcg cctcttttga cggaaagtaa
     8221 tacagcagat tggtttttga aacacccgcc aactctgcga tctgctccag ccttgtgccg
     8281 tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa gaatcgcttt tttcttcgca
     8341 ctgactgcgc gcgaacgttt acccgttgtt ttcactgcac cttgcgtcat gcgctctccc
     8401 ctcttgtaga ttggcaacag gatagcaaaa cccccgcccg gcctcgacct tctgcactct
     8461 catcgcgcac ggatgcatgt tttatgtgca actgttttga ccgtttagtc cactttttac
     8521 cagatatttt agataacgca atgttaacat attaataaca ttcacttttt aaaaactggc
     8581 atccactttg caaacaagcc aatacgcggc tgaaaaggat atgcaggatg cagcgccccg
     8641 tctgactttc actttacgag acgaagagag gttgatgatg aaaattggcg tattcgtacc
     8701 tattggcaac aacggctggc tcatttcgac ccacgcgccg cagtacatgc cgacctttga
     8761 actgaataaa gccatcgtgc aaaaagcgga gcactaccat tttgatttcg ccctgtcgat
     8821 gatcaaactg cgtggctttg gcggcaaaac tgagttctgg gatcacaacc ttgagtcgtt
     8881 caccttgatg gcggggctgg cagccgtgac ctcgcgcatt cagatttacg ccaccgccgc
     8941 caccttaacg ttacctccgg caatcgtcgc ccgtatggcc gcaaccatcg actccatctc
     9001 tggcgggcgt tttggcgtca acctcgtgac tggctggcaa aagcccgagt atgagcagat
     9061 gggtatctgg cctggcgatg actatttctc ccgtcgttac gactatctca ccgaatatgt
     9121 tcaggtgttg cgcgacctgt ggggcacggg aaaaagcgat tttaaaggct attttttcac
     9181 catgaatgat tgtcgcgtca gtccgcaacc gagtgtcccc atgaaagtga tctgcgccgg
     9241 gcaaagcgac gctggcatgg cgttctccgc tcagtatgcc gatttcaact tctgtttcgg
     9301 caaaggcgta aatacaccta cggctttcgc cccgaccgct gtgcggatga aacaggccgc
     9361 agagcaaacc gggcgcgacg ttggctctta tgtgttgttt atggtgattg ccgatgaaac
     9421 cgacgatgcc gctcgtgcca aatgggaaca ctacaaagcg ggcgcggatg aagaggcgtt
     9481 aagctggcta accgaacaaa gtcagaaaga tacccgctcc ggtactgaca ccaacgtccg
     9541 tcagatggca gatcccactt cggcggtaaa catcaatatg gggacgttag tcggttctta
     9601 cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgccg aaggcgtgct
     9661 gttaaccttc gacgattttc tgtcgggaat cgaaaccttc ggcgagcgca ttcaaccact
     9721 gatgcagtgc cgcgcccatc tccctgtgct gactcaggag gtggcatgat gacgacatta
     9781 accgctcgac cggaagccat taccttcgat ccgcagcaaa gtgcgctgat cgtggtggat
     9841 atgcaaaacg cctatgccac gccaggcggc tacttagatc tcgccgggtt tgatgtctca
     9901 accactcgcc cggtcattgc caacattcaa accgccgtga ccgcagcgcg agcggcaggg
     9961 atgttgatca tctggtttca aaatggctgg gatgaacagt atgtcgaggc tggcggcccc
    10021 ggctcaccga attttcataa atcgaacgcc ttgaaaacca tgcgtaagca gccgcagcta
    10081 caggggaaat tgctggcgaa aggctcctgg gattatcaac tggtggatga actggtgccg
    10141 cagcctggcg atattgtgct gccgaagccg cgctacagcg gtttcttcaa tacgccgctg
    10201 gacagcattt tgcgcagccg cggaatacgc catctggttt tcaccggcat cgctaccaac
    10261 gtctgcgtcg aatcgacgct acgcgacggc ttttttctgg agtatttcgg cgtggtgctg
    10321 gaagacgcaa cacaccaggc ggggccggaa tttgtacaga aagccgcgtt gttcaatatc
    10381 gaaacctttt ttggctgggt cagcgacgtc gaaacgttct gcgacgcgct ttctcccacg
    10441 tcctttgctc gtatcgctta aggagtttaa cgatgccaaa atccgtaatt attcccgctg
    10501 gcagcagcgc accgctggcc cccttcgttc ccggcacgct ggctgatggc gtggtgtatg
    10561 tctccggtac gctggctttt gatcaacaca ataacgtgct gtttgccgat gacccaaagg
    10621 cgcaaacccg ccacgttctg gaaactatcc gcaaggtgat cgagacagcg ggtggcacga
    10681 tggcggatgt gaccttcaac agcatcttta ttaccgactg gaaaaattac gccgcgatta
    10741 acgaaatcta cgccgagttt tttccaggtg ataaaccggc gcgattctgc attcagtgcg
    10801 gactggtaaa acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc gccaagtgag
    10861 gccgcgatga aactttcact ctcacctccc ccttatgctg atgcacccgt agtggtgttg
    10921 atttcgggtc ttggcggtag cggcagttac tggttaccgc aactggcggt gctggagcag
    10981 gagtatcagg tagtctgtta cgaccagcgc ggcaccggca ataatcccga cacactggca
    11041 gaagattaca gtatcgccca gatggcagcg gaactgcatc aggcgctggt agccgcaggg
    11101 attgagcatt acgcggtggt cggccatgcg ctcggtgcgc tggtgggaat gcagctggcg
    11161 ctggattatc ccgcgtcggt aactgtgctg gtcagcgtta acggctggct acgaataaac
    11221 gcccatacgc gccgctgttt tcaggttcgc gaacgattac tgtatagcgg cggcgcgcag
    11281 gcatgggtgg aagcgcagcc gttgttcctc tatcccgccg actggatggc ggcccgcgca
    11341 cctcgcctgg aggcagaaga cgcgctggca ctggcgcatt ttcagggcaa aaataattta
    11401 ctgcgtcgac ttaacgccct caaacgcgct gactttagtc accatgcgga tcgcatccgc
    11461 tgcccggtgc aaatcatctg cgccagtgat gatctgctgg tgccatcagc atgttccagt
    11521 gaacttcatg ccgccctgcc cgatagccag aaaatggtga tgcgctatgg cggacacgcc
    11581 tgcaacgtga ccgatcccga aacgtttaat gctctgttac tcaacgggct tgccagcctg
    11641 ttacatcacc gtgaagccgc cctgtaagga attgctatga acgaagccgt tagcccaggt
    11701 gcgcttagca ccctgttcac caatgcccgc actcacaatg gctggcggga gacacccgtc
    11761 agcgatgaga cgttacggga gctttatgcc ctgatgaaat gggggccgac atcagctaac
    11821 tgttctccgg cacgtatcgt gtttatccgc actgcagagg gaaaagaacg tctgcgcccg
    11881 gcgctttcca gcggcaattt gcaaaaaacc ctgaccgcgc ccgtcaccgc tatcgtcgca
    11941 tgggacagtg aattttatga acggttaccg caactgtttc ctcacggcga tgcccgcagt
    12001 tggtttacct ccagcccaca acttgccgaa gaaacagcgt ttcgcaacag ttccatgcag
    12061 gcggcctatc tgatcgtcgc ctgccgtgcg ctggggctgg atactggccc gatgtcgggt
    12121 tttgaccgtc aacacgtgga cgacgccttt tttgcgggta gcacgctgaa gagcaatctg
    12181 ctgattaata tcggctatgg cgatagcagc aaactttttg cgcgcctgcc acgtctgtcc
    12241 tttgaagaag cctgcgggct gttgtaagga gcaaactatg aacattgtcg atcaacaaac
    12301 ttttcgcgat gcgatgtcct gcatgggtgc ggcggtcaat atcatcacca cggacggtcc
    12361 agccgggcgc gccgggttca ccgccagcgc cgtctgcagc gtgaccgata cgcctcccac
    12421 attactggtg tgcctgaatc gtggggcgtc cgtctggccg gtattcaatg aaaatcgaac
    12481 gctgtgtgta aatacactta gcgccgggca ggagccgctt tcaaaccttt ttggcggcaa
    12541 aacgcccatg gaacaccgct ttgccgccgc ccgctggcag actggtgtga ccggatgccc
    12601 gcaactggaa gaggcgctgg tttcgtttga ctgccgtatc agccaggtgg tgagcgtcgg
    12661 cacccacgac attttgtttt gcgccatcga agcgattcat cgtcacacca caccctacgg
    12721 gctggtgtgg ttcgatcgca gttatcacgc gctgatgcgc cctgcttgtt aacctcctta
    12781 aggagacagc tgatggcaat gttcggtctt cctcactggc agttaaaatc gacctctaca
    12841 gagagcggcg tggtcgcgcc ggatgaacga ttgccgtttg ctcagacggc aataatgggc
    12901 gttcaacacg cggtggcgat gtttggcgcg acggtgttaa tgccgattct gatggggctg
    12961 gatcccaatc tttccatttt aatgtcaggg atcggcacgt tactgttttt ctttattacc
    13021 ggcgggcgcg tgcccagtta tctcggctcc agcgcggctt ttgttggtgt ggtgatcgcg
    13081 gcaacgggct ttaacggcca ggggattaac ccgaatatca gcatcgcgct gggcgggatt
    13141 atcgcctgtg ggctggttta taccgtcatt ggcctggtgg tgatgaaaat cggtacgcgc
    13201 tggattgaac ggctaatgcc gccggtggtg acgggtgcgg tggtgatggc gattggcctg
    13261 aacttagcgc ctatcgcggt aaaaagtgta tcggcttcag gctttgatag ctggatggcg
    13321 gtaatgacgg tgctgtgtat tgggctggtg gcggtgttta cccgtggcat gatccagcgg
    13381 ttattgattc ttgtaggatt aattgtcgcc tgtctgctgt atggcgtgat gaccaacgtt
    13441 ttagggctgg gaaaagccgt cgattttact ctcgtcagcc atgctgcctg gttcggtcta
    13501 ccgcattttt ccactcctgg ttttaatggt caggcaatga tgctgattgc gcccgtggcg
    13561 gtgattctgg tggcggaaaa cttaggccat ctcaaggcgg tcgccgggat gaccggacgc
    13621 aatatggatc cgtatatggg acgggcattc gtcggtgatg ggctggcgac gatgctttct
    13681 ggttctgtcg gtggcagcgg cgtcaccacc tatgccgaga atatcggggt aatggccgtg
    13741 acaaaagtct actcgacgct ggtgtttgtc gctgctgccg tcatcgccat gctattgggc
    13801 ttttcaccga aatttggcgc gctgattcat accattccgg ccgcggtgat tggtggtgca
    13861 tcaattgttg tgttcgggct gattgccgtc gcaggcgcaa ggatatgggt acaaaaccgt
    13921 gtcgatttaa gccagaacgg taatttgatt atggtcgcag taaccttagt gctgggcgcg
    13981 ggtgattttg ctctcacgct gggcggtttt acgttgggag ggatcggtac agcaaccttt
    14041 ggcgcgattt tactcaatgc gttattaagc agaaggttgg ttgacgttcc accgccagaa
    14101 gttgttcatc aggagccata aacagaaaac cgccgggatt accggcggtg atgacttttt
    14161 gacgagctta agaaaaaaat gcattcgtca ttgattagcc cggctagttg tcgcttttgc
    14221 cgtgactgct cttgccacct tttttacctg cttcagatgc gcgctgcgga tcattcttga
    14281 aattaccccc gctgtgctgt ccaccttttt tacctgcttc tgatgctctt tcgcggtctt
    14341 ctgcaaaatt gccggaaccg cctcgatggt ttgccattac tgacctccgt ctgggtatag
    14401 acatcatatt gcatagaatt aacgcggcgt atggcggctg ccatattttc gctacgtggg
    14461 actaagctta gtgaactgac gtgtttctac ccgcgtttgg taatatcctg caacagcttc
    14521 ttagcgagaa tagatatcat ctccaataat tatccataag ccgctcttat gattaaaaaa
    14581 acgcgatgag ataaaaatgt gtcattttgc gacaaaattg cgtgcttgtg aaagttgtta
    14641 taaatcaaat aagtggttgt gaaatttgca ctctgaaaag gacgtcttat ctttaaataa
    14701 gtggtagcga atcgctacgg aatagagata acacgaggag tggttagaaa tggctaaagt
    14761 tctggtgctt tattattcca tgtacggaca tattgaaacg atggcacgcg cagtcgctga
    14821 gggtgcaagc aaagtggatg gcgctgaagt tgtcgttaag cgtgtaccgg aaaccatgcc
    14881 gccgcaatta tttgaaaaag caggcggtaa aacgcaaact gcaccggttg caaccccgca
    14941 agaactggcc gattacgacg ccattatttt tggtacacct acccgctttg gcaacatgtc
    15001 cggtcaaatg cgtaccttcc tcgaccagac gggcggcctg tgggcttccg gcgcactata
    15061 cggaaaactg gcgagcgtct ttagttccac cggtactggc ggcggtcagg aacaaaccat
    15121 cacatccacc tggacgaccc ttgcgcatca cggcatggtg attgtcccca ttggctacgc
    15181 agcgcaggaa ttatttgacg tttcacaggt tcgcggcggt acgccgtacg gcgcaaccac
    15241 catcgcaggc ggtgacggtt cacgccagcc cagccaggaa gaactgtcta ttgctcgtta
    15301 tcaaggggaa tatgtcgcag gtctggcagt taaacttaat ggctaatctt caacaggagg
    15361 atacgcatgc caactcaaga agcgaaagct catcacgtcg gtgaatgggc atctctgcgc
    15421 aatacgtcgc cggaaatagc tgaggccatt ttcgaagtcg ccgggtacga cgaaaaaatg
    15481 gcggaaaaga tttgggaaga aggtagtgat gaagtgttag tcaaagcctt tgctaagacc
    15541 gataaagatt cgcttttttg gggcgaacag accatagaac gtaaaaacgt ttaaccagcg
    15601 actcccccgt ttctcacggg ggagttttct gttatttcac cgcttcattc aacacgctat
    15661 caaacttatc catcgggcag aaaccgttgg catctatcgg gcaaccgctt aattccagcg
    15721 tcacacgctg cgcaggtgcc tgcagggtta acgcatcggc attacgtaac tgttccgcac
    15781 tctggtacac atactcgatt ttcatcaaat cgcgattggc tttgctgtca tgccaacgct
    15841 ggaaaacgat tttgccgcca atcggcgtgc gttcgttctg gtcatgcaac tgatacggtt
    15901 tgaaatccag cgccgttaac agagaggcaa tgttggagtc atgcccaact aacaccgtga
    15961 tttttggtgc actggtgcga tcggtgacca gggctttgtc gatataactg accagcggtt
    16021 tcgcaacatt gcgcgccact tccggtgagg taaacaggct gtcctggtaa ccgtttttca
    16081 gcttcgacaa caccttccac tgctggtcag atttgatttc tccccaggcc acctgatcca
    16141 tcggaaaacc ttcgtagtat tgcaaagtaa acgcatctac cagcgagttg ccgactttca
    16201 gcggcccgga aacacctggt tcttgttgat acttcgcgct aaaggtattt ttgccatcca
    16261 ccagcgaaca ctgctgtttc tctttacagg caggggaatc tttatagtta acgatttttt
    16321 ccagtagctg gtagctgtcg gtaagctgga gtttgctgag ctctttctcc attgccgcca
    16381 ccgcctgttc actgaatgcg gcggaatcat cggtgatcac cgggttaaag gttgggtcca
    16441 tggtgcccat tttttcctgg tgatgcacag gaatatcaca ccccgggaat gcgccggtaa
    16501 taaagaactg tgcggtcgcg acagtacgtt gcagactatt ggcataggca taaacggtgt
    16561 ccggaggcgg gcattccccc gatttcacca tcccctgctc tgccagccat tcacgcatgt
    16621 aatggcccat atacacttcg agcacgccac ctttggtggt gagttgccca ccggggacgt
    16681 cccattctgg ccatttattc ggcgtcgact gctccagcac actgccattg ttcgccagcg
    16741 gcgcacgtaa gttatggcgg ctcatcatga gcacttgctg tagctgatag ccttccggta
    16801 cggtttgtgc ctgagcattt gaagcgagta aaactatccc tgccacagtt gcggtgatta
    16861 gcgttttgtt cattcctggc acctcttttg ttatcaatca ctaaagagtg tgacagaaat
    16921 atgacttttt tccgggaaca ggttcgcaaa atggcttgtt gaacgcctgt ttttctatat
    16981 acatcttcaa aaacaggcaa ggtttgacgc aaactatgcc tgattagccc ctgcctcgcg
    17041 tacgccgctg ataattgcac ctgtggctaa acagaatatc tgcggcgtta ccaatacaga
    17101 gacaatattt aacaataaaa aagtgtgaga ctaataacaa tggtttttag catatgcctt
    17161 tgccattaaa ttgcataacg atttaaatag tcaagttgcg cattcatttc cttattgttt
    17221 aacttgtaag cctgagcaaa aagttcagga gggaggtaaa cgtcataatg attgccggtg
    17281 tttattaaat aaccatactc aagatcttca tctgttgaaa aggattcatt ccccaaggag
    17341 attgatgcca cattactatt aacaataaat aatgagcaaa tgctatctga aagagcagtg
    17401 aggaaatttt tcacctcatt aatcctcttc tctgggtcgg tgtctaaaac aaggcgtgcc
    17461 ttgttatcat taaatataaa ctcgggtaaa tgcagtatac attttttgtc attcaagcaa
    17521 ctatcaataa attctttttt aaaaaacaga aagttccatg acataagact accctccccc
    17581 tgaattttgt tgtcagattg tagatcaacg ccaaataacg tctttacatc ttccctgaga
    17641 ttatagaatg ccgtatcaac aagagcctcg atcgtttgtt tatcaacaat tttaataaac
    17701 tcttcgtata cttgcttttg cgtcttatgg ctgcccagcg cccatgaata ctcatgttct
    17761 tttaaaacca gtatggccct taacagacaa tagccatcgc catctacagg agcatatttt
    17821 aatcctaatg cctggttagt ttcacgctga ataaaagata taccagataa atcaatacca
    17881 gtatctaaat cagatattcc cggcgcattt atccattctg gctgaagact aatattttca
    17941 tcatctaaac aatactcaga aaaaacatca tcacacgcat taatgttttc aatatattcc
    18001 ataaagccat accaataata acctttaaaa tctgtgatag actctgaccc tctgcccggt
    18061 aaggacactc taacaatatc aacatcttca acatattgta tagtatatgt aatacctcct
    18121 gaaacccctg aataaatttg tttatcatta gtacgcaatg ttttcataaa taatgataaa
    18181 tcattatcat tctgtatttc ctcaaattct ttttctagtg aatcgcattc caggcatgga
    18241 atgtagtctt cagcttgttg acgtctgatt tctttataca accgcataaa acacaccagt
    18301 gtgttttcaa aaagcgtgat tgaatatgtt ttattttttt cgtactcatt ttttatatcg
    18361 ttccaggctt gcttcaaata attatttgca gtgcaactaa ttccatgtat attacccata
    18421 tatagcgcct ataaaattta ataaataatg acgcactagt aaaacttaaa gtgcctgttt
    18481 caactatcaa aaatcgctca ccctttttca cctgtttaaa atatgttcag caccccacct
    18541 tgaggcgacc tcctctccgc gatgatttca ataacatatt ctgtgttggc atatgaaatt
    18601 ttgaggatta ccctacactt ataggagtta ccttacaggg gttccttcaa tttgtgttga
    18661 tttacgcgag ataacgctat ggaattaaag gattattacg ccatcatggg cgtgaaaccg
    18721 acggacgatc tcaagacaat caagaccgcc tatcgtcgac ttgcccgcaa ataccatcct
    18781 gatgtcagca aagaaccgga tgccgaagcc cgcttcaaag aggtcgctga agcctgggaa
    18841 gtgttaagtg atgaacaacg tcgcgctgag tatgatcaga tgtggcaaca tcgcaacgat
    18901 ccgcaattta accgtcagtt ccaccatggc gacggtcaga gttttaacgc cgaagatttt
    18961 gacgatatct tctcgtcaat tttcggtcag catgcccgcc agagccgtca acgccccgcc
    19021 acacgcggcc acgatattga aatcgaagtg gcggtattcc tcgaagaaac gcttactgag
    19081 cataagcgta ccatcagcta taacctgccg gtttataacg cctttggcat gatcgaacag
    19141 gaaatcccga aaacgctgaa tgtgaagatc ccggcaggcg tcggcaatgg tcaacgcatc
    19201 cgcctgaaag gccaggggac gccgggcgaa aacggcggtc caaatggcga tttgtggctg
    19261 gtgattcata ttgcgccaca tccgctgttt gatattgtcg gccaggatct ggaaattgtg
    19321 gtgccggtta gcccgtggga agcggcgctg ggtgctaaag tcaccgttcc aacactgaaa
    19381 gaaagcattt tgctgactat cccgcctggc agccaggccg ggcaacgatt gcgcgttaaa
    19441 ggcaaaggtc tggtgagcaa aaaacagacc ggcgatctgt atgcggtact gaaaatcgtg
    19501 atgccgccga aaccggatga aaacactgcc gcgttgtggc agcaactggc agacgcccag
    19561 tcgtcttttg atccacgtaa agattggggg aaagcataat ggctaatgtt acggtgactt
    19621 ttactattac cgaattttgc ctgcataccg gcatctctga agaggaactg aatgaaattg
    19681 tcggtttggg ggtggttgaa ccgcgtgaga tccaggaaac aacctgggta tttgacgacc
    19741 atgcagccat cgtggtgcaa cgcgcggtgc gcctgcgtca tgaactggct ctggactggc
    19801 cggggatcgc ggtggcgctg acgttaatgg atgatattgc gcacctgaag caggaaaacc
    19861 gcctgctgcg ccagcggctt tcccggtttg tagctcatcc gtgatgtgtg tttgccggat
    19921 gtgatgacgc atccggcaac tgccccataa tgcctgatgc gacgctaaac gcgtcttatc
    19981 aggccaacaa acgacgttat ctgttttggt ggtcgccctc aactaacacc agcaataact
    20041 ggcttagtgc cgcgtaaaaa ccaaagctgt catactgatg gcaacgcgca acaaactctg
    20101 gtaaccattg ccgcagcgcc gtgagtgttt tttgccgtaa actgtcgatt cttcgcgcag
    20161 gaaccgtccc ctctcccagc gaaaaatgca gatgactcaa caactcgaga tagatcgcca
    20221 gatgatctgc cggttcgttg aaattgccgc tggtttccat ccctgcctca actaacaagc
    20281 gtttaatttc ttgctcgtcc tgtttgtagg ccgatgcata cggcagcgcc gcttgtttgt
    20341 cagtcatcag aaacaggccg caaaagtcag cggccagttc cagacgggca tcgtcacgta
    20401 ctgtcagagt ggcaacacgg ttttccagct catccatcgc cgcagtgagc ggcggttcgc
    20461 ttttcagcaa cgaaaaccat tcagccatct gcgcactggc gatttgcgtc agttgttcat
    20521 cgtccagttc acgggagaac aactgcgcca gccaggcgta aacacaggca atctgttgtg
    20581 ctgtcagcgt ggtcatgatt tcacctgcga cgcgggaaca tattcgcact gcgccaccat
    20641 ctccacgggg ccgttaaacg ccgtcacctg atccactgtt ccgttgtact tctcaatttc
    20701 caccagcgta gtgtgcgcac tggtcgcctg cgccagctgc gaagtaccga tgtcgattgt
    20761 caacacgttg gggttaccgt atttgcacag cgcaccaggc tcgccgcctt tatctggatc
    20821 gtaccatgcc ccttcgtgaa ttcgcgccac gccgggtgca tagcgatcag aaactactgc
    20881 ccctgccaac acctgaccgc gagcgttaaa gacgcgtacc acatcaccgt tacgaatacc
    20941 gcgcgcgctg gcatcctgcg ggttaatgaa cactggctct ttacccgcta tcgtatattg
    21001 ctgacgcaga gtttccgact cacataactg cgagtgaagt cggaaatccg gatgcacaga
    21061 ttgcagatgc aacggatacg tctgcgagcc gggcccaccg tgggagcgtt cgattttctc
    21121 aaaccacatc ggatgcccct gacaatcgtc gtagttcata tcggcgatgg ttttcgagta
    21181 gatctcaatc aggccactcg gcgtgcccag cggttcgaga tccggatctt cgcggaatgc
    21241 ctggtggcga acaaacatct gcggatggtc aaactcgacg tactctttgt tattccagaa
    21301 gtcatcaaac gctggcaaat gaacgccgcg tcctttgcct tgctgaacac cttcctgcca
    21361 gatgcgtttc agccagccca tttcgtccag cccttcggta aaggcttctt cgcgattaaa
    21421 gcggcggcac agctcgcgga aaatatcaaa gtcgttgcgc gcctcgaact gcggcggcac
    21481 gacctgtttc atggcgataa tgccacggtt ggagtggttg ccgtactggt cgagatcgtt
    21541 acgctcaaac tgcgtggtcg caggcagcac gatatcggca aagcggcagg ttgaggtcca
    21601 ctggttatct atggcgataa ccgtttccag cttgcgccag ccttcaataa tgcggttgat
    21661 ctgctgatgg cgatggaatg ggttagttcc ggcaaaaata cacattttca gcggcggcag
    21721 ttttaccgat ttaccgttcc agttgatcac tttccccggt tcgaggatcg catcgataaa
    21781 acgggcaatc ggaatggtgc tgctgtagcc tttgtaatca ctgttgtcgt gaacaggcgg
    21841 aatcgacgta gagccggaga aaccactcag aataacgcct ttacgccccg gcgtgcctgc
    21901 gccgttatag tgccagccaa aaccaaagcc accacctggc aggccaattt gccccagcat
    21961 cgccgccaga accacaatca tccacgccca ctgttcaccg tgctgcatac gctgtacgca
    22021 ccagccagca ataatctgcg ttctgttcgc cgccatctgc cgcgccagtc cacgaatggt
    22081 ttcggcatca atgccggtca gtttttcagc ccatgcggca tctttcggct gaccgtcttt
    22141 ctcacccagc agatacggca ggaactgctc aaaacccaca cagtagttag cgaggaagtt
    22201 tttatcgtac aggtttttac tgtacagcgt atacgccagc gccagttgca gcggcacgtc
    22261 agtttgcggg ttaaccgcaa tgtgcttcac atgctcgcgc cccagatact catgggtgga
    22321 tgtaacaacc ggatcgatgc taatgacctc aatttcaccg gcggcgactt tcgctttcaa
    22381 ctgcgcgtaa tattcataaa catcgtgatc cgggcaccac cagttcgctt gctggttttt
    22441 cagcaaatcg gagccccaca gcacaatggt tttgctgttc tgcaatacca gcggccagga
    22501 ggtttgctgt tcatacactt ccatcgaacc gactacgcgc ggcaggatca cctgcgcagc
    22561 accagtagag taatctccgc ccgtaccaac gctattacca tgcaaggcaa tagctttcgc
    22621 cagcatcccc gaagcgttat ggaacatccc cgtcgattgc caaccactgg cggtcagcaa
    22681 ggcactcggc ccgtgagttt tctgtacgcg ttccagttct tcatagaaca tgtcgagggc
    22741 ttcatcccag ctcacacgca caaaacggtt atcaccgcgc tgggaggtgt cgctcagatg
    22801 gcgcttacgc agccagtcca cgcgtaccat cggataacga atacgcgccg cgttgtgtac
    22861 atgatccggc aatccggcaa tcattttcga cggatattta tccagttcga acggttttgc
    22921 cgccacaaag cgaccatcct tcaccgtcgc gcggatagcc ccccagtgcg acccggtaag
    22981 aatgccctct ttcgagatga cagcctcagt cgccgcttgc gccgcagtcg cacggcgcgg
    23041 cgttaacaat gacggcccca gcatcccggc gacggttaag ccgccgagtt gtgccagaaa
    23101 acgccgacgt gatgcctgaa agagatcgtt attgttcatt atttttcttc cttcttatcg
    23161 ccgtgagcct tacctgcggt gtcagacgca ttcatttgca gatatttcaa caaggtgcgt
    23221 tcttcacgct tatcgaggct ggtaaagcca atcatgccgt tgagcgtgcc gatccaaccg
    23281 ttagcgtcaa agtgggagat ttccggtgcg ccgtggcact ggttacaggt gccgttgtac
    23341 agcgaatccg cataagccca gatcggtttg atatcgttca ccatgtcgcc tttcttcatc
    23401 cacgcggtgg cctgcaactt gctccactcg gtattggtgt cggctacggt ggttttctcc
    23461 agcgttttca cctgctgctg cacatcacca cgaatcgagg caacaaagat gcgtttacct
    23521 gggaattggg tgagtacacg ctgacgtccg gcgctttctg tccagccggt aatttcaatt
    23581 tgcagccagt cgccgtcacg tttaaggact ttcacttccg aggcaggcag cagagaacca
    23641 gaggcttctt tatcgccttt cgccgcataa atcggcttaa tatcaataga gtacagcttg
    23701 tcgccactgt cattagcacc ggcgcgcagc tcatcgaact gcttacggaa gccgctactc
    23761 atatccggta actggtgggc aataccttta tgacagtcga tgcaggattg attatctttc
    23821 gctgccacct tcatctgacg tgccgcttca ggatgctgct tcgcatgatc catcgcgtcg
    23881 tagttatggc aggagcggca ggttgccgag ttgttttctt tcattcgtgc ccattcacgc
    23941 tcggcaagtt ccgcgcgttt ggcttcgaat ttttcaggtg tatcaatgga gtgggcaata
    24001 aaggtctggt agatatcgtt gctcgcttcc agtttacgct tcaccatgcc tggaatatcc
    24061 ggcgggatat gacagtcatg gcattcagct cgcacgccgg aggcgttctg gaaatgcacc
    24121 gactgtttat attcttcata caccggttgc atactgtggc aactgacaca aaattcggtt
    24181 gtgctggtga ctttgatccc aacgtgtggc aatacaatca gcgcaatgcc aatcacaatc
    24241 ccaatagcga ccagcgccag taccgaccaa cgagcactgg gtcggcgtag cgcgttccag
    24301 agtttccgca taatagcccc tgtaaaatta tggtttagtg aagcgatctt aatgagcaaa
    24361 tatgaacagc ggcactggtc aggatgaatg gcttacggca gaatatgaac agatatgaac
    24421 agaatgagta aaaccctctg atgccacatc acattgttat tgttgaagat gagccggtta
    24481 cccaggcgcg attacaatcc tacttcactc aggaggggta taccgtttcc gttacggcga
    24541 gcggtgccgg gctacgggaa attatgcaga atcagccggt ggatttaatt ctgctggata
    24601 tcaacttgcc cgatgaaaat ggcctgatgt taacccgcgc cctgcgagaa cgctcaacgg
    24661 tggggattat tctggttacc ggacgcagcg atcggattga ccgtattgtt gggctggaaa
    24721 tgggcgcaga cgattacgtc accaaaccgc tggaactgcg cgaactggta gtacgggtaa
    24781 aaaatctgct ctggcgaatc gacctcgcgc gacaagttca accgctcact caggacaact
    24841 gctatcgctt tgcgggttat tgcctgaatg tgtcgcgcca tacgctggag cgggatggtg
    24901 agccgattaa actgacccgc gcagagtatg aaatgttggt ggcatttgtg accaatccgg
    24961 gagaaattct cagccgtgaa cgtctgcttc gtatgctctc tgcgcgtcgg gtggaaaacc
    25021 ctgacctgcg caccgtcgat gtgttaattc gtcgtttacg ccataaactc agcgcggatt
    25081 tactggtgac gcaacatggc gaaggttatt tcttagccgc tgatgtgtgc tgataaaaat
    25141 agaccggacg aaatccccct ggcgacagcg agcggcggat atgttcgcgg tcggcatttt
    25201 tcggcgtcag aactaaaatc ggtgggctga cattatcaga caccgattgc ccctgtaatt
    25261 gcctgatggc ctgctcaact gccagttccc cctgccagac catttgatcg ctggcagcca
    25321 taatcactct tccccgcttc agcccgcgat acacctgatg tgaaagataa aacgacacca
    25381 cggtaagcgg cgttttcagg ttacgccctt cccccattgc cgcctctgcc gcaatggccg
    25441 ttccggcaac gacgtcgatt tctggatggc gttccagcat ctcctgcaac aggttacgct
    25501 ggatttcaat atcgttatca ccaagcgcaa tatcaacaat acgcaccggg cttccggcaa
    25561 tggctgcgcg aaaaccctcg accatctcct tactgccccc ggcgttatcg ggtccgggca
    25621 tcaacagcac gttcagtggt ttaccgtgcg cccattgcac caaatatcgc ccaggttgat
    25681 agcccatctg aaaccagggt acaccaacgc ggcttttcac ctggggagca tcaatagcat
    25741 ttaccagttc gatcaccggc agacttgcta cctgcttttg cagatcggga aatgacgtcg
    25801 tgctactacc gagcaaaatg gcctctgcgc cccactgttt acactggtcg atttgtgctt
    25861 gctgggtagc caactggctg tagccgcctg cctccagcac ttttaaatcc acaccgtagc
    25921 ggcgagcagc ctcctgcata ccatagttca acgataacca gtatgaatct ttcaggctgg
    25981 gataaagcgc gcacagtttc caggcgcgtt tggctttaag cggcgtagag gattgcaccg
    26041 tgaaatgctg cgcatcatgc cagcgcaaca ggttatcagc cgaaaatgcc ggcaacatga
    26101 aaagggaaag aagtaaaaat agcagtacgc gcatgatagc ctcatcaata ataaggcttt
    26161 atgctagatg cattcctctt tgcgactcaa catttttcac cttaagtgca ccgaccgtga
    26221 atttaaccct gacccgaaga ctctggatgg gctttgccct gatggcgctg ttaaccctga
    26281 ccagtaccct ggtgggatgg tacaacctgc gctttatcag ccaggtggaa aaagacaata
    26341 ctcaggcatt gattcctacc atgaatatgg cgcgccagtt gagcgaagcc agcgcctggg
    26401 aacttttcgc cgcgcagaac ctgaccagtg ccgataacga aaagatgtgg caggcgcagg
    26461 ggcgaatgct caccgcacaa agcctgaaga ttaatgcgtt gctgcaagcg ttacgggaac
    26521 aaggttttga taccaccgct attgaacaac aggagcagga gatctcccgt tcgttacgtc
    26581 agcaagggga actggtgggg cagcgtttgc aactgcgcca gcaacaacag cgactcagtc
    26641 agcagatagt cgctgccgcc gatgagatcg cacgcctggc gcaaggtcag gcgaataatg
    26701 cggcaacctc cgccggagcg acccaggccg ggatttacga tttgatcgaa caagatcagc
    26761 gtcaggctgc tgaaagtgca ctcgatcggc tgattgatat cgatcttgag tatgttaacc
    26821 agatgaatga actgcgcctt agcgctctgc gggtgcagca aatggtgatg aatctggggc
    26881 tggagcagat ccagaaaaat gcgccaacgc tggaaaagca gctcaataat gcggtgaaaa
    26941 ttctgcaacg tcggcaaata cgcattgaag atccgggagt tcgtgctcag gtcgcaacaa
    27001 cgttaactac cgttagccaa tatagcgatt tgctggcgct gttccagcag gacagtgaaa
    27061 tcagcaatca cctgcaaact ctcgcccaaa ataatatcgc ccagttcgcg cagtttagta
    27121 gcgaagtcag tcagctggtc gacaccattg agctgcgcaa tcagcatgga ctggcgcatc
    27181 tggaaaaagc cagtgcacgc gggcaataca gcctgttatt gctggggatg gtttcacttt
    27241 gcgcactgat tctgatcctc tggcgcgtgg tttatcgctc agtcacgcgt ccacttgccg
    27301 aacaaacgca ggcgctgcaa cggctgctgg acggtgatat tgactcccct tttccggaaa
    27361 ccgctggcgt acgggagctg gataccatcg ggcggctgat ggatgcgttt cgcagcagtg
    27421 ttcatgcact gaatcgccac cgcgaacagc tggcggcgca ggtcaaagca cgtacagctg
    27481 aattgcagga actggtgata gaacaccgac aggcacgggc ggaagcagaa aaagccagcc
    27541 aggcaaaatc ggcgtttctg gcggcgatga gccatgagat ccgcacaccg ctgtacggta
    27601 ttctcggcac cgcacagcta ctggcagata accccgcact taacgcccag cgtgatgatt
    27661 tgcgggcgat taccgactct ggcgaatcgt tgctgaccat cctgaacgat attctcgatt
    27721 attcggctat cgaagcaggc ggcaagaatg tttcggtcag cgatgaaccc tttgaaccgc
    27781 gaccgctgct ggaaagtacc ctgcaattaa tgagtggacg ggtgaaaggt cgcccgattc
    27841 gcctggcaac ggcaattgcc gacgatgtac cgactgcgtt aatgggcgat ccgcggcgta
    27901 ttcgtcaggt cattaccaac ctgttgagca acgccctgcg ttttactgac gaagggcaga
    27961 tcgttttacg tagccgtact gatggtgagc aatggctggt cgaagtggaa gacagcggct
    28021 gcggtattga tcccgcgaaa ctggcagaaa tcttccagcc atttattcag gtaagtggca
    28081 aacgcggcgg caccgggctg gggctgacta tcagtagccg tctggcccag gcgatgggcg
    28141 gcgaactgag cgccaccagc acgctggagg ttggaagctg tttttgttta cgcttgccgt
    28201 tacgtgttgc cacagcaccc gcgccaaaaa cagccaatca ggcggtgcgt ctggacggtt
    28261 tacgtttgct gttaattgaa gataacccgc taacccagcg aattaccgtt gagatgctga
    28321 acaccagtgg tgcgcaggtt gttgctattg gcaatgccgc gcaggcttta gagacactgc
    28381 aaaatagcga accgtttgct gccgcactgg tggattttga tctgccggat atcgacggca
    28441 ttacccttgc ccggcaactg gcacagcaat atccgtcgct ggttttgatt ggctttagcg
    28501 ctcatgtcat tgacgaaacg ctgcgccagc gtaccagttc actatttcgc gggattatcc
    28561 ctaaaccggt gccgcgtgaa gtgctcggtc agttactgga gcactatctc caactgcaag
    28621 tcaataacga tataccactt gatgtatcgc aactcaatga agatgctcaa ttaatgggga
    28681 cggagaagat ccacgaatgg ctggcattat ttaaacaaca tgccctgccg cttctcgatg
    28741 aaatcgacat tgcccgcgcc agccaggaca gcgagaaaat aaagcgtgcc gcgcatcagc
    28801 taaaaagcag ttgctcgagt ctgggaatgc gcagcgccag ccagctatgc gcacaactgg
    28861 agcagcagcc attatctgcc ccccttccac acgaagaaat tacacgcagt gttgccgctc
    28921 tggaagcatg gttgaataag aaagacctga acgcgatttg aatcaatatc agccagttaa
    28981 gaataactag aatagtggca tttggtatgc aacttaacgg actggcaaaa tcatggcaga
    29041 gaaaaaaaga acccgctggc agcggcggcc aggcacgacg ggcggcaaat taccgtggaa
    29101 tgactggcgc aatgccacga cctggcgtaa agcgacgcaa ttcttactat tggcaatgaa
    29161 tatttatatt gccatcacgt tctggtattg ggtgcgctat tacgaaacgg caggtagtac
    29221 gacatttgtc gccagaccgg gaggtatcga aggctggcta ccgattgccg gtctgatgaa
    29281 tctgaaatat agccttgcaa caggtcagtt accgtccgtc cacgccgccg caatgctgtt
    29341 attggtcgct tttatcgtca tcagtctatt actcaaaaag gccttttgtt catggttatg
    29401 cccggttggt acgctttctg aattaatcgg cgctctcggt aacaaacttt ttggtcggca
    29461 atttgtcctt ccccgctggc tggatattcc tctgcgcggt gtaaagtatt tgctgttgag
    29521 tttttttctc tatatcgcgt tattgatgcc cgctcaggcg attcactatt ttatgttgtc
    29581 gccctacagc gtggtgatgg acgttaaaat gctcgatttc tttcgttata tggggaccgc
    29641 gacattaatc agcgtgaccg ttttgctgat tgccagcctg tttattcgcc atgcctggtg
    29701 tcgttatctt tgcccatatg gcgcgctgat gggcgtggtt tcgctattgt cgccgtttaa
    29761 gattcgtcgc aatgccgaaa gttgtatcga ctgtggcaaa tgcgcaaaaa attgcccatc
    29821 acggatcccg gtcgataaat taattcaggt acgaacagtg gaatgtaccg gctgtatgac
    29881 ttgcgtagag tcatgtccgg tagcctcaac attgaccttt tcactgcaaa aacctgcggc
    29941 aaataaaaaa gcctttgcgt tgtctggctg gttaatgacg ctactggttc tggggattat
    30001 gtttgcggtg attggttacg caatgtatgc gggagtatgg caaagcccgg tgccggagga
    30061 attgtaccga cgcttaattc cgcaagcgcc aatgattggt cactaaatta gcaacaagcg
    30121 gaacatcacc tcgcacgggt gatgttcaca aaatcacatt attttgattt tgacatcata
    30181 actttcaagc ccggtcattt tttctcgtgc ggtgaagcga atttcagaga cttctttccc
    30241 tgtattcttg ttcagctcgg cgattttttg cgcgataaag tcggcgattt ccgtttcggc
    30301 ttgttttttt aactcttcaa tattcactgt taacctcgtc tggtcttgaa agacagcaac
    30361 acgccagtaa ggtgttggtt cccggtaccc tggtcaggta agaaatgtta taagccatct
    30421 ccccgaaaga ttcaataatc aacaggttat cgcttactat acatacagcg ttgaagaaaa
    30481 ataaggcaga tataaaaaag gctccacgag ggagcctgat attattttgt gaaatccagc
    30541 ggtgcatggt cagtgcgtat ttgtagcgcg acgttatcaa aggtgatcat cgtcgattta
    30601 caaaaaatac acttagcgcc caaagggttt cgctccgtca catcgaatgc cgatgtgcgg
    30661 tactgtgaac catgacaaca tggacagcga aaatggagat gttgagtaat aacggcaacc
    30721 ttagagcgta actacgttcg ctgccgcggg gccacgttgc ccctgctcaa tagaaaattc
    30781 aactttctga ttttcgttca gcgtgcggaa ttcattgctc tggatggcgg tgaaatggac
    30841 gaaaacgtct ttgctgccat catcaggagt gataaagcca aaacctttat ctgcgttaaa
    30901 ccattttact aaaccagtca ttttattaga catattttac ttccttcatt ttgggccttt
    30961 cggcaaacag ggctttaata cagaaactac ttagtgttca ggagagagac tcaaaagaag
    31021 gggataatgc ctgataatga gaactgcttt agtaaactac tttgtatgct gtctgtcttt
    31081 caaaccgacg cagctattaa cgcatgataa ttgagattaa gcaaatttta ttttagccgt
    31141 ccggcggtct taaatatcgc tggctttatt ttattaaccc cgtatagtgc agcgcagtat
    31201 tttcagtaag gaatttgttt gtcccgtaaa atgacaggaa ttgtcaaaac ctttgatcgc
    31261 aaaagcggca aaggattcat tatcccctcc gacggtcgta aagaagttca ggtccatatt
    31321 tccgcattca ctccccgcga cgcagaagtg cttataccag gattacgcgt ggaattttgc
    31381 cgcgtaaatg gcctgcgagg accaacagcg gcaaatgttt atctctctta atatgacgct
    31441 gcttgtttaa agctgactgg ttttattggc atagttaaat agtcaacacc atgtattata
    31501 acacaagtta ctttttcacc ggaaaactaa tccatttaac cgaatatctc tacaactaat
    31561 a
//
LOCUS       DAZGBC010000041        31258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGBC010000041 DAZGBC010000000
VERSION     DAZGBC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.041"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            452..2041
                     /gene="purH"
                     /locus_tag="ACHK1J_001898"
     CDS             452..2041
                     /gene="purH"
                     /locus_tag="ACHK1J_001898"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO7911174.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            2053..3342
                     /gene="purD"
                     /locus_tag="ACHK1J_001899"
     CDS             2053..3342
                     /gene="purD"
                     /locus_tag="ACHK1J_001899"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO7911175.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(3339..4664)
                     /gene="zraR"
                     /locus_tag="ACHK1J_001900"
     CDS             complement(3339..4664)
                     /gene="zraR"
                     /locus_tag="ACHK1J_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO7911176.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(4661..6037)
                     /gene="zraS"
                     /locus_tag="ACHK1J_001901"
     CDS             complement(4661..6037)
                     /gene="zraS"
                     /locus_tag="ACHK1J_001901"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO7911177.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            6275..6700
                     /gene="zraP"
                     /locus_tag="ACHK1J_001902"
     CDS             6275..6700
                     /gene="zraP"
                     /locus_tag="ACHK1J_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO7911178.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(6702..7397)
                     /gene="yjaH"
                     /locus_tag="ACHK1J_001903"
     CDS             complement(6702..7397)
                     /gene="yjaH"
                     /locus_tag="ACHK1J_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO7911179.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(7410..7682)
                     /gene="hupA"
                     /locus_tag="ACHK1J_001904"
     CDS             complement(7410..7682)
                     /gene="hupA"
                     /locus_tag="ACHK1J_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO7911180.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            complement(7869..8459)
                     /gene="yjaG"
                     /locus_tag="ACHK1J_001905"
     CDS             complement(7869..8459)
                     /gene="yjaG"
                     /locus_tag="ACHK1J_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO7911181.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            complement(8502..9173)
                     /gene="nfi"
                     /locus_tag="ACHK1J_001906"
     CDS             complement(8502..9173)
                     /gene="nfi"
                     /locus_tag="ACHK1J_001906"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO7911182.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            complement(9183..10247)
                     /gene="hemE"
                     /locus_tag="ACHK1J_001907"
     CDS             complement(9183..10247)
                     /gene="hemE"
                     /locus_tag="ACHK1J_001907"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO7911183.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGDVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            complement(10287..11060)
                     /gene="nudC"
                     /locus_tag="ACHK1J_001908"
     CDS             complement(10287..11060)
                     /gene="nudC"
                     /locus_tag="ACHK1J_001908"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO7911184.1"
                     /translation="MDRIIEKLDHGCWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            11155..11631
                     /gene="rsd"
                     /locus_tag="ACHK1J_001909"
     CDS             11155..11631
                     /gene="rsd"
                     /locus_tag="ACHK1J_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO7911185.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     regulatory      11683..11783
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11864..13759
                     /gene="thiC"
                     /locus_tag="ACHK1J_001910"
     CDS             11864..13759
                     /gene="thiC"
                     /locus_tag="ACHK1J_001910"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO7911186.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGAHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     gene            13759..14394
                     /gene="thiE"
                     /locus_tag="ACHK1J_001911"
     CDS             13759..14394
                     /gene="thiE"
                     /locus_tag="ACHK1J_001911"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO7911187.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQTADWRLATAQLLEIAGVGDE"
     gene            14387..15142
                     /gene="thiF"
                     /locus_tag="ACHK1J_001912"
                     /pseudo
     CDS             14387..15142
                     /gene="thiF"
                     /locus_tag="ACHK1J_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            15126..15326
                     /gene="thiS"
                     /locus_tag="ACHK1J_001913"
     CDS             15126..15326
                     /gene="thiS"
                     /locus_tag="ACHK1J_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO7911188.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINHQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            15328..16098
                     /gene="thiG"
                     /locus_tag="ACHK1J_001914"
     CDS             15328..16098
                     /gene="thiG"
                     /locus_tag="ACHK1J_001914"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO7911189.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            16095..17228
                     /gene="thiH"
                     /locus_tag="ACHK1J_001915"
     CDS             16095..17228
                     /gene="thiH"
                     /locus_tag="ACHK1J_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO7911190.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(17638..18176)
                     /gene="yjaZ"
                     /locus_tag="ACHK1J_001916"
                     /pseudo
     CDS             complement(17638..18176)
                     /gene="yjaZ"
                     /locus_tag="ACHK1J_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
     gene            complement(18389..22612)
                     /gene="rpoC"
                     /locus_tag="ACHK1J_001917"
     CDS             complement(18389..22612)
                     /gene="rpoC"
                     /locus_tag="ACHK1J_001917"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO7911191.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(22689..26717)
                     /gene="rpoB"
                     /locus_tag="ACHK1J_001918"
     CDS             complement(22689..26717)
                     /gene="rpoB"
                     /locus_tag="ACHK1J_001918"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO7911192.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPIDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(27037..27402)
                     /gene="rplL"
                     /locus_tag="ACHK1J_001919"
     CDS             complement(27037..27402)
                     /gene="rplL"
                     /locus_tag="ACHK1J_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO7911193.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            complement(27469..27966)
                     /gene="rplJ"
                     /locus_tag="ACHK1J_001920"
     CDS             complement(27469..27966)
                     /gene="rplJ"
                     /locus_tag="ACHK1J_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO7911194.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            complement(28258..28962)
                     /gene="rplA"
                     /locus_tag="ACHK1J_001921"
     CDS             complement(28258..28962)
                     /gene="rplA"
                     /locus_tag="ACHK1J_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO7911195.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(28966..29394)
                     /gene="rplK"
                     /locus_tag="ACHK1J_001922"
     CDS             complement(28966..29394)
                     /gene="rplK"
                     /locus_tag="ACHK1J_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO7911196.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(29553..30098)
                     /gene="nusG"
                     /locus_tag="ACHK1J_001923"
     CDS             complement(29553..30098)
                     /gene="nusG"
                     /locus_tag="ACHK1J_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO7911197.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(30100..30483)
                     /gene="secE"
                     /locus_tag="ACHK1J_001924"
     CDS             complement(30100..30483)
                     /gene="secE"
                     /locus_tag="ACHK1J_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO7911198.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            complement(30713..>31258)
                     /locus_tag="ACHK1J_001925"
     CDS             complement(30713..>31258)
                     /locus_tag="ACHK1J_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO7911199.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLS"
BASE COUNT         7239 a         8546 c         8103 g         7370 t
ORIGIN      
        1 cactgtcgcg aggtggcgta tattacgctt tcctctttca gagtcaaccc tgaatttcag
       61 ggtttttctc ttcaaccgaa ccggctgttt gtgtgaagtg attcacatcc gccgtgtcga
      121 tggaggcgca ttatagggag ttctccgcag gtcgcaatag aaaaattgca taaaaataac
      181 tgactgctgc attccccagc aaaaccccgc tttatacctt tttacacaca gagttatcca
      241 caatcatcag cgttattttt gtatttttca cacggtaacc actgtcaaaa ttgtgatcac
      301 cattgaaaga gaaaaattcg cgagcgttgc gcaaacgttt tcgttacaat gcgggcgaaa
      361 aataaggatg ccccgttagg ggcgttagct gagtttttcg cgaaaaattc agctaacgct
      421 ctctgtaata gtcaaatcca ggggatttac catgcaacaa cgtcgtccag tccgccgcgc
      481 tctgctcagt gtttctgaca aagccggtat cgtcgaattc gcccaggcac tttccgcacg
      541 cggtgtggag ctgctgtcta cagggggcac tgcccgtctg ttagcagaaa aaggtctgcc
      601 ggtaaccgaa gtttccgatt acaccggttt cccggagatg atggatggac gcgtgaagac
      661 cctgcatccg aaagtacatg gtggcattct gggccgtcgc ggccaggacg atgccattat
      721 ggaagaacat cagatccagc ctatcgatat ggtggttgtt aacctgtatc cgttcgccca
      781 gacagtggcc cgtgaaggtt gctcgctgga agatgcggtt gagaacatcg atatcggcgg
      841 cccaacgatg gtgcgctccg ccgccaagaa ccataaagat gtcgcaatcg tggtgaagag
      901 cagcgactac gacgccatta ttaaagagat ggatgacaac gaaggatcgc tgacgcttgc
      961 aacccgtttc gacctcgcca tcaaagcctt cgaacacact gccgcctacg acagcatgat
     1021 tgccaactac ttcggcagca tggttccggc ttaccacggt gaaagcaaag aagccgccgg
     1081 tcgcttcccg cgcacgctga acctgaactt cattaagaag caggatatgc gctacggcga
     1141 gaacagccac cagcaggctg ccttctatat agaagagaat gtgaaagaag cctccgttgc
     1201 taccgcaacc caggttcagg gtaaagccct ctcttataac aacatcgccg ataccgatgc
     1261 ggcgctggag tgcgtgaaag agttcgccga gccggcatgt gtgattgtga agcacgccaa
     1321 cccttgcggc gtggctatcg gcaattccat tcttgatgct tacgatcgcg cgtacaaaac
     1381 cgacccgacc tccgcattcg gcggcatcat tgcctttaac cgcgagctgg atgcggaaac
     1441 cgcacaggcc atcatttctc gtcagtttgt tgaagtgatt attgcgccgt ccgccagcga
     1501 agaagccctg aaaatcaccg ccgccaaaca gaatgtacgc gttctgacct gcggtcagtg
     1561 gggcgagcgt gttccgggcc tcgatttcaa acgcgtgaac ggcggtctgc tggttcagga
     1621 tcgcgatctg ggcatggtcg gtgcggaaga actgcgcgtc gtcacccaac gtcagccgac
     1681 cgaacaggaa ctgcgtgatg cgctgttctg ctggaaagtg gcgaagttcg tgaaatccaa
     1741 tgctatcgtc tatgccaaaa acaatatgac catcggtatt ggcgcgggcc agatgagccg
     1801 cgtgtactcc gcgaaaatcg ccggtattaa agctgccgat gaaggcctgg aagtgaaagg
     1861 ttcctcgatg gcttctgacg cgttcttccc gttccgcgac ggtattgatg ccgccgcggc
     1921 tgcgggcgtg acctgtgtaa tccagcctgg cggttctatc cgtgatgacg aagtgattgc
     1981 cgccgccgac gagcacggta ttgcgatgct cttcaccgac atgcgccact tccgccatta
     2041 atggagcaat agatgaaagt attagtgatt ggtaacggcg ggcgcgagca cgcgctggcc
     2101 tggaaagcgg cccagtcgcc gctggttgag actgtttttg ttgctccggg taatgcaggc
     2161 actgcgctgg aacccgcgct gcaaaacgtt gctatcggcg tgaccgatat cccggcgctg
     2221 ttggatttcg cacaaaacga aaagattgat ctgaccatcg tcggcccgga agcgccgctg
     2281 gtgaaaggcg tggtcgatac cttccgcgct gccgggctga aaatcttcgg cccaaccgca
     2341 ggtgcggccc aactggaagg ctcaaaagcg tttaccaaag atttcctggc ccgccataag
     2401 atccctacgg cggaatacca gaacttcacc gaggtagaac ctgcgctggc gtatctgcgt
     2461 gagaaaggcg cgccaatcgt cattaaagcg gacggtctgg ctgccgggaa aggcgttatc
     2521 gtggcgatga cgctggaaga agcggaagcg gctgttcacg atatgctggc gggcaacgct
     2581 tttggcgacg cgggtcatcg catcgttatc gaagagttcc tcgatggcga agaagcgagc
     2641 tttatcgtga tggtggacgg cgagcatgtg ctgccgatgg ctaccagcca ggatcacaaa
     2701 cgcgtaggcg ataaagatac cggaccaaac acgggcggga tgggcgctta ctcccccgcg
     2761 ccggtagtaa ccgatgaagt tcatcagcgc accatggaac gtattatctg gccaaccgtg
     2821 aaaggcatgg cggcggaagg caacacctac accggttttc tctacgcggg cctgatgatc
     2881 gacaaacagg gcaatccgaa ggttattgaa tttaactgcc gctttggcga tccggaaacc
     2941 cagccgatta tgctgcgcat gaagtccgat ctggttgagc tctgcctggc ggcctgtgaa
     3001 ggcaaactgg acgagaaaac gtcagagtgg gatgaacgtg cttctctcgg cgtggtgatg
     3061 gctgcgggtg gatatccggg cgattaccgc accggcgatg tgatccacgg cctgccgctg
     3121 gaagaagtgg ctgacggcaa agtgttccac gcgggcacga aactggcgga taacgagcag
     3181 gtagtgacca gcggcgggcg cgtactgtgc gtcaccgcgc tgggtcatac cgtggcagaa
     3241 gcgcagaaac gcgcttatgc cttaatgacc gatattcact gggacgactg cttctgccgg
     3301 aaagatatcg gctggcgcgc tatcgaacgc gagcagaact aacgcgacag ttttgccaat
     3361 agcgttttgc gcgtgatccc taactgacgg gcggcttcgg ttttgttgcc gcccgttttc
     3421 tccagtgccg ccagaatcac ttctttttcg acttccacca gcggctgaat atcctgactt
     3481 tgccccagcg ggatcggcgt accggcaatc gccagcggca gctcgcgttc ggaaatatat
     3541 tccccggtca gcagcaccac cgcccgttcc accgcgtttt ccagttcacg aatatttccc
     3601 ggccagtcgt agtggatcag caaatccatc gcctgtggcg taaagccttt taccgccttg
     3661 cgattacgct cggcaaagcg ctgaagaaaa tggccagcca gcagagggat atcttcccgc
     3721 cgttgccgca gcgatggtac ttcaatcgcc accacattca ggcgatagta gagatcctgg
     3781 cgaaaacgcc cggcattcac ctctgcggca agatcgcgat gggtcgccgc aatcagccgg
     3841 acatcaaccg agatagtctg gttgctaccg acacgctgaa cttcgcgctc ctgaatcgca
     3901 cgcagcagac gcacctgcat catcggcgag atatcgccaa tttcatcgag aaacagcgtg
     3961 ccgccgtccg cctcaacaaa gcgcccttcc cggcgtttat cggctccggt aaacgcccct
     4021 ttttcgtgac cgaacaattc agattccagc aaggattcgt taagtgccgc acagttgagc
     4081 gttaccagcg gtttttcgct acgtgcgcta ctggcgtgaa tcgccctggc gaccagctct
     4141 ttaccggtgc cggaatcgcc gtggatcagc accgtggctt ccgatggcgc gacgagggcg
     4201 atttcactga gcaggtgttg catcgccggg cttttaccga ccataccgaa ctggctggca
     4261 gtcaccgcag gtgtttcagc atcaatactg tgcgtatgcg cgagcgcctt ttccagcgtc
     4321 gcctgtaggt tatcgaaatc cagcggcttg atgagataat ccagcgcccc agttttcagt
     4381 gcctctaccg ccgtctcgac gctggagtac gcagtcataa tcagcaccgg aattgccggg
     4441 tttaacgctt tgatctcttt cagcgtggcg atgccgtcca tctccgccat tcgcacatcg
     4501 caaagcacaa gatcaaaaac ctgttcccgc acctgctcca gcgcctgtcg cccgctgttc
     4561 gccagcgcga cgttatagcc ccagccgcgc agtaaagcct gcaaaatagt gcagtggcta
     4621 atgtcatcat ccaccaccag aatatcgata ttatcgtgcg tcatccttgt gggtccttac
     4681 gcgtaatatt gaccggaagc cagagggtga acgttgagcc ttttccctcc tggcttgcga
     4741 cctgaattgt accaccgtgt tgttcaacaa tattatgcac gaccgccagc cccagtccgg
     4801 tgccttcggc tttagtggtg aagtacggag tgaagatggc atcaagctga tctgccgcaa
     4861 ttcccttacc gctgtcggta acgctgattt tcacgcccgc gccgctttcg ctggccgtca
     4921 cgctaatcac gccatgctga ccaatcgcct gaatagcatt gagatagaga ttcaacagga
     4981 cctgagtcag cctgtccgga tcggcctgaa tttccggtaa tgtgtcgttg gcggtaaagc
     5041 gtaactggat ctcccggctg tttgcatcct ggcttaccag ctgtaatgag tggttaatca
     5101 gcgtgttgag atccaccgcc tgcaaagcca gatgcgttgg cttaaccagt tccagcaact
     5161 cgcttaccac gcggtttaaa cggtcggcct ctttcgccat cacctgcgcc agttgatgcg
     5221 cttctccccc tgcaggcgcg cgctcggcaa agtattttgc cagtccttta atcgaggaaa
     5281 gtgggttacg gatttcgtgg gcaacgcctg ccgcaagatg ccccagcgcc accagcttct
     5341 ctttgcgctt catttcatct tgtagaagct ggcgcgagcg cagatagcgg cgataccaga
     5401 agaatgacaa tacgcttgcc agcaagaccg tcgccagggc gaagaggata atcagggtgt
     5461 ttctctgctc acgatcttcg gcactcacaa tattactggc gtcgaaagcg atgaaaattg
     5521 cttgtggtgt cgcggcatac tgctgcatat ggcgcatccg gtgcattcca gcagcaaaca
     5581 tcggttgaaa ctggcgataa atttccagaa caggctcacc gtctgcgcta tcgatccgcc
     5641 gccacgcttc ttcatctccc ggatgtaact gctgcatttc ctgcggggaa taaagctgtt
     5701 ttcccaccat gccggagttg ctatgcatca cgattgttcc ttgttcatcc gtgaccgcaa
     5761 accaacgtac tccaggctgc ccggccattt cttccagtaa ggcctgctgc tgcgcatgat
     5821 gcatgcgcat ccccatgccg acgcgcgagc cggattcgag agcacgaata agtacactgc
     5881 ctttttccag cagcgtttgt ctggcagcgg cagtctcgcg cccataatcg cgaatcaccg
     5941 tcaccgcaaa caaccctacc agcccaacaa tgaccacggg gaggatcgcg cttaaccatt
     6001 tagctaagga gtctttagaa cgttgcataa aacgcatctt cttctttgcc tgctcatccc
     6061 tttttgacag cagaaatcat gccatctttt atcagcgctt accctgcgct gtaacacaaa
     6121 ggcttaagtt tcaatgagta aaaatgactc gctacccgca gcaggcgagt catttttact
     6181 cgtttatcat gccagattac ccgtcatatc agcgtttcat cgttggcacg gaagatgcaa
     6241 tacccgaagt aagacaacca ctggaggatt aaccatgaaa cggaacacga aaattgccct
     6301 ggtgatgatg gcgctttcag caatggcgat gggatcgaca tctgcatttg ctcacggcgg
     6361 acacggtatg tggcagcaaa atgccgcgcc tttgaccagc gaacaacaga cagcgtggca
     6421 gaaaatccat aatgactttt acgctcaaag cagcgcactg caacagcaac tagtgacgaa
     6481 gcgttatgaa tacaatgccc tgttagccgc gaacccaccg gatagcagca aaattaacgc
     6541 ggtcgccaaa gagatggaga atttgcgtca gtcgttagat gagttacggg tgaaacggga
     6601 tattgcgatg gctgaagcgg gtattccgcg cggagccgga atgggcatgg gctacggcgg
     6661 ctgcggtggt ggcggtcata tgggtatggg ccactggtaa atcagaacgt tttctcgttg
     6721 ggttgccagc gacagaaatc agagtttgcc actaacagta attgcgaacc ttcgggcgct
     6781 tccagccatg ccacgctgac atcaacagaa gagcggctct ggcggcgctc gatatgcgct
     6841 atcgcctgcg aatctaaatc aggtttaatg gtttgccctt cgcaggtggt cacggtctgt
     6901 tccatcgcat gccagcgccc ctggcgcagc acgactctcc cctgacgtaa ggcatcgcta
     6961 atctggcgga tctgatcagc ccgataacgg tataaatcga tttggtcgtt agaaagctgc
     7021 tgcttttccc catcggattc acgttgcata aagctcagat caccgcgatc gtcaaaacgc
     7081 gcgcgaatgt tcaccggcgg tttgctgtaa acattgatat tgatgagcgt gaggttatcg
     7141 ccctgccagc gatactctcg cgtcgtggta tcgccactgc gccatgggct aaacacggca
     7201 agcagatgca cattatcgcc gctgtctttt cgccagatgc gtaccgcgcc ctggtcttca
     7261 gcaaatccac tggcggtaaa gggaggaagt gacgagttgt gactacaggc agtgagaaac
     7321 agcgcacctg ccagcatcaa tgatcgacgc cagaaagaca aaaggggtga aaccacccct
     7381 tcgttaaaac tgttcactgc cacgcaatct tacttaactg cgtctttcag tgccttgcca
     7441 gaaacaaatg ccggtacgtt agctgcggca attttgattt ctttaccggt ctgcgggttg
     7501 cggccagtac gctcagcgcg gtggttcact ttgaaggtac cgaaaccaac cagttgtaca
     7561 gcatcgcctt ctttcagaga ctcagtaatt gcagccagag tggactccag agcagcttta
     7621 gcctgggttt tggacagttc tgctttctct gcaattacat caatcagttg agtcttgttc
     7681 ataagttatc cttacaatgt gtttatcgct tgctaagcat cgagtgcgac ggaaatgcca
     7741 gaatagcact ctcctgcata cacgcaccga tagccacttt ttttcgcccc ccaaatgtag
     7801 accagacggg gggcgaaagg gaagactcca ggtatgacaa atcaggcgtt aaatcacgtt
     7861 ttctggtctt attgctgaaa aattatacca atattgctct cacccgcctc acgcaggtct
     7921 gccctaaggc cttttatcag ctcgatatcg cgttcttcgc actcagctaa aagtcggaat
     7981 atttcccact gaatgtccca ttcttgctct acagctgggt tttctttgag ctcttcatcg
     8041 ctcatttcgc gaccagcctg agtcatttcc agcatcgcaa cggtcgtgat ggaggtctta
     8101 ctcacttcca ccgcgtgttc gagcgtttca ccactcaaac gcgaatggac cagttcactt
     8161 aacgccacgc aggcatcgat tgccggataa acgccgtaca gatcgaaatc gtcggctgaa
     8221 ggaatcgctt cttcaaattt ctccagttgg ctgtcgaaat ttacttttgc gtctttgacg
     8281 gtcagcgttt cccagatgag atcgagaata cggcggtaaa tttgcccatc gccaaaaccg
     8341 gtttgctggc agaacatggc gtaattgggg tacatgcgtt cgcataagca agccatgaaa
     8401 gtgacatgct gccagctttc caggcgctcc agacgcagat gaattgggtt ttgtaacatg
     8461 ttgagttctc aaatacggaa attacccgca gtttacctga attagggctg atttgctgta
     8521 tagcgcacga acgctggacg ttccgaggcc accgcgtccg cccagcgcgt tggctccggc
     8581 agacgatagc ctttcatgca gcgttgtacc cacgccagcg cgctgtccac gctgacccga
     8641 tggccggtag cgataaacaa cgggttacag cgcgctttgc tgcgccagac ccaggccagc
     8701 tgctcgcctt tatccatcag tggggccagc gcgcccggtt cgctggagag cggttcgaat
     8761 ttaccgcata gtcgtttttt cgctacgcca atggtcggca catccaccag caaaccaaaa
     8821 tggctggcga cgccaagacg gcgaggatgc gagatcccat gaccatcgac aaacactaaa
     8881 tccggttttt gtgacagcat ctcccacgct gccaggagcg caggatattc gcggaaggaa
     8941 agaaaacctg gaatgtaagg catggtggta gcgatgcggg ccactttata ctccaccagc
     9001 tcaagcgagg ggtatttcag cagcaccatc gccgctcgcg tcacttctcc gccctgctca
     9061 aacccgacat cggctccggc gatcagatcc ggcggatcct tatcgagtcg atcctcgcgg
     9121 atcacagaag aagccagttc gatttgttga gcgcgtaatg acgcgagatc cataatcgct
     9181 ccttagcggt gatactgttc agacagtcga tgcactgcct ccacgaacac gccagcatgt
     9241 tctggcggca catcctgatg aatgccgtga ccaaggttaa agacatgacc ttcgccgtga
     9301 ccgaaacctg caagtatagt cgctacttct tcttcaatgc gggcaggcgg cgcgtacagc
     9361 atcgacggat ccatattacc ctgcaacgcg actttattgc ccacacggcg gcgcgcatcg
     9421 gcgatgtccg ttgtccagtc gaggcccagc gcatcgcaac cggtttctgc catggcttcc
     9481 agccactgtc cgccgccttt ggtaaacagc gtgaccggta cgcggcgacc gtcgttttca
     9541 cgcagtaaac catcaacaat tttatgcatg taataaagcg agaactgttg ataatcgcgc
     9601 ccggtaagca cgccgcccca ggtgtcgaaa atcatcacag cctgagcacc ggctttaatc
     9661 tgcgcattca gatacaaagt gacgcttttc gccagtttat cgagtagagc gtgcagcgcc
     9721 tgcggatcgg catacatcat ttttttgatc acggtgaagg ctttgctgct gccgccttcc
     9781 accatgtagg tcgccagcgt ccacgggctg ccggaaaaac caatcagcgg gacgtcgcct
     9841 ttcagttcgc ggcgaatggt acgcaccgcg ttcatcacgt aacccagctc atcttccggg
     9901 tccggaattg gcagtttatc gacgtcagct ttgcaggtga ctggcgaggt aaaacgcgga
     9961 ccttctccgg cctcaaaata gagccctaac cccatcgcgt ccggcacggt gaggatatcg
    10021 gaaaagagga tcgccgcatc cagcgggtag cgacgcagcg gttgcaaagt cacttcgcac
    10081 gccagctcgg cgtttttgca cagcgacata aaatcgcctg cctgggcgcg cgtggcttta
    10141 tattccggta gatagcgacc cgcctggcgc atcatccata ctggagtgac atcaacgggc
    10201 tggcgcagca gcgcccgcag ataacgatcg tttttaagtt cggtcatttt ggctgttcct
    10261 tagtgcgtca ggcggtcagt gtatcatcac tcgtactctg cccgacacat cgccaccgta
    10321 tcttctatca gacggcgcgc cacggtgccg ggcggcggga gtaacggcaa atcgtcatag
    10381 cgataccagt tcgcctcgag caattctttc gggtcgatca cgatgtcgcc gctgtcatat
    10441 tccgccataa acgcggtcat taaagactga ggaaacggcc acggctggga agtcacataa
    10501 cgcaagtttt taactttaat tccgctctct tccatcactt cccgcgcgac cgcctgctcg
    10561 agggtttcgc ccacttcgac gaatccggca agtaccgtat ggacaccgtt acgatggcgg
    10621 gtatgctggg cgaggaggat cgaatcatcg cgacggatgg caacaataat gcagggggcg
    10681 atttgcgggt agtaacgctc acggcaatgg ctgcacagca tcgcccattc ggttttgctt
    10741 ggatacattt catgcccgca gtaaccacag tatttatgcg atcggtaaaa ctccgccagt
    10801 tgcacgccac gtccggccag ttgaaacagc ccaacatcga gatcaatgac ctgacgtacc
    10861 gaccccatat cgtgacgccg ctgctgttgt attaaccaaa caggttcccc ctgccattcg
    10921 ccgatttgta gtgcgcgctg acccacaaga tcgaaatttg ccgcttcgcc atatggcaat
    10981 tctcccttcg gcaaccataa tttttgttca tggctgacga cccagcagcc gtgatctaat
    11041 ttttcaatta tacgatccat agctcttgca ctacctttgc atcactggca tgtttaacat
    11101 ggtttttaca tttctcactg agcagttttt gaatacaaac ttgcggagtc aatcatgctt
    11161 aaccagctcg ataacctgac ggagcgcgtc agaggaagta acaaactggt tgatcgctgg
    11221 ctacatgtac gtaagcatct gctcgtggct tactacaatc tggttggcat taagcctggc
    11281 aaagaatcgt acatgaggct aaacgaaaaa gcccttgatg atttttgtca gagcctggtc
    11341 gattacttgt ctgccggaca tttcagtatt tatgagcgca ttcttcataa gctggaaggc
    11401 aacgggcaac tcgcgcgcgc cgcgaagatt tggccgcaac tcgaagccaa tacccaacag
    11461 attatggatt actacgattc cagcctggaa accgctatcg atcatgataa ttaccttgag
    11521 tttcaacagg ttttatctga catcggcgag gcgctggaag cgcgctttgt gctggaagat
    11581 aagctgattc tgctggtgct tgacgccgcc cgcgtcaaac atcctgcttg agttctgcgc
    11641 tgttaacgcg taatttacat tcaacgcccc gtttgtaggg ctaatttctt gtcggagtgc
    11701 cttaactggc tgagaccgtt tattcgggat ccgcggaacc tgatcaggct aatacctgcg
    11761 aagggaacaa gagttaatct gctatcgcat cgcctttgcg gcgatcgtct cttgcttcat
    11821 ccgtcgtctg acaagccacg tccttaactt tttggaatga gctatgtctg caacaaaact
    11881 gacccgccgc gaacaacgcg cccaggccca acattttatc gacaccctgg aaggcaccgc
    11941 ctttcccaac tcaaaacgca tttacatcac tggcgcacac cccggcgtgc gcgtgccgat
    12001 gcgtgagatc cagcttagcc cgacgctaat cggcggcagc aaagaacagc cgcagtacga
    12061 agaaaacgaa gcgattccgg tctacgacac ctccggcccg tatggcgatc cgcagatcgc
    12121 cattaacgtg caacaagggc tggcaaaact acgccagccg tggatcgatg cgcgcggcga
    12181 taccgaagaa cttaccgtgc gcagttccga ttacactaaa gcgcggctgg cagatgatgg
    12241 cctcgacgag ctgcgtttta gcggcgtatt aacgccaaaa cgcgccaaag caggacgccg
    12301 cgtcacacaa ctgcactacg cccgccaggg catcatcaca ccggaaatgg aattcatcgc
    12361 catccgcgag aatatgggcc gcgagcgcat ccgtagcgaa gttttacgcc accagcatcc
    12421 gggaatgagc tttggcgcac gtctgccgga aaatatcact gcggaatttg tccgtgatga
    12481 agttgctgcc ggacgtgcga ttatcccggc caacattaat catccggaat cggagccgat
    12541 gattattggc cgtaacttcc tggtaaaagt taacgccaat atcggcaact cagcggtcac
    12601 ctcttccatc gaagaagaag tggaaaagct ggtatggtcc acgcgctggg gagcggatac
    12661 ggtgatggat ctctccaccg gtcgctatat tcacgaaacc cgcgagtgga ttttgcgtaa
    12721 cagcccggtg ccgatcggta cagtgccgat ctaccaggcg ctggagaagg ttaacgggat
    12781 cgccgaagat cttacctggg aagcgttccg cgacacgctg ctggaacagg ccgagcaagg
    12841 tgtggattac ttcactatcc atgcgggggt gctgctgcgc tatgtgccga tgaccgcgaa
    12901 acgcctgacc ggtatcgtct cgcgcggcgg ttcgattatg gcgaaatggt gcctctccca
    12961 tcatcaggaa aatttcctct atcaacactt ccgcgaaatt tgtgaaatct gtgccgctta
    13021 tgacgtttcg ctgtcgctgg gcgacggtct gcgccccggt tctattcagg acgccaacga
    13081 tgaagcacag tttgccgagc tgcatacgct gggcgaactg accaaaattg cctgggaata
    13141 tgacgtgcag gtgatgattg aaggcccagg ccacgtgccg atgcagatga tccgccgcaa
    13201 tatgaccgag gagttagagc actgccacga agcgccgttt tacactctgg ggccgctaac
    13261 taccgatatt gcgccgggct atgaccactt cacgtcgggg attggtgcgg cgatgattgg
    13321 ctggtttggc tgcgcgatgc tctgttacgt aacgccaaaa gagcatctgg gtctgcctaa
    13381 taaagaagat gttaagcagg ggcttatcac ctataagatt gccgcccacg ccgctgacct
    13441 ggcgaaaggg catccgggcg cgcaaattcg cgataacgcc atgtcgaaag cccgcttcga
    13501 atttcgctgg gaagaccagt ttaatctggc cctcgacccg tttaccgccc gcgcttatca
    13561 cgatgaaacc ctgccccaag agtccggcaa agtcgcccat ttttgctcca tgtgtggacc
    13621 gaaattttgc tcgatgaaaa tcagccagga agtgcgtgat tacgccgcca cgcaaactat
    13681 tgaaatggga atggcggata tgtcggagaa cttccgtgcc agaggcggag aaatctacct
    13741 gcgtaaggag gaagcgtgat gtatcagcct gattttcctc ctgtaccttt tcgtttagga
    13801 ctgtacccgg tggtggacag cgtacagtgg atcgaacgtc tgttggatgc aggcgtacgt
    13861 actctccagc tacgcatcaa agatcggcgc gatgaagagg tggaagccga tgtcgtggcg
    13921 gcaattgcgc tgggccgccg ctataacgcg cgattgttta tcaacgatta ctggcggctg
    13981 gcgatcaagc atcaggcgta tggcgtccat ttggggcagg aagatttgca agccaccgat
    14041 ctcaatgcca tccgcgcggc aggcctgcgg ctgggcgttt cgacacatga cgatatggaa
    14101 atcgacgtcg cgctggcagc acgcccctct tatatcgcgc tgggacatgt gttcccgacg
    14161 caaaccaaac agatgccttc tgcaccgcag gggctggaac agctggcacg gcatgttgag
    14221 cgcctggcgg attatcccac cgtggcgatt ggcggtatca gtctggcgcg tgcgcctgcg
    14281 gtgatagcaa cgggtgtcgg cagtatcgcc gtcgtcagcg ccattactca aaccgcagac
    14341 tggcgtttgg caacggcaca gttgctggaa attgcaggag ttggcgatga atgaccgtga
    14401 ttttatgcgt tatagccgcc aaatcctgct cgacgatatc gctcttgacg ggcagcaaaa
    14461 actgctcgac agccaggtgc tgattatcgg tcttggcggg ctgggtacac ctgctgcgct
    14521 atacctggcg ggcgctggcg tcgggacgct ggtactggca gatgacgacg atgtgcattt
    14581 aagcaatctg caacgacaaa tcctctttac cactgaagat atcgatcgcc cgaaatcgca
    14641 ggtcagccat cagcgactga cacagttgaa tcccgatatt caactgacag cattacaaca
    14701 acggttaacg ggtgaggcgt taaaagatgc ggttgcacaa gccgatgtgg tgctcgactg
    14761 taccgacaat atggcgactc gccaggagat taatgccgcc tgcgtggcac tcaacacgcc
    14821 gcttatcacc gccagcgcgg tcggatttgg cggtcagttg atggtactga cgccgcccta
    14881 ggagcagggg tgttaccgct gcctgtggcc agataaccag gagccagaac gcaactgccg
    14941 cacggcgggc gtggttggcc cggtggtcgg ggttatgggc actttgcagg cactggaagc
    15001 cattaagtta ttaagcggta tagagacgcc tgcgggagaa ctccgactgt tcgacggtaa
    15061 gtcgagccag tggcgcagcc tggcgttgcg ccgcgccagt ggttgcccgg tatgcggagg
    15121 aagcaatgca gatcctgttt aacgatcaac cgatgcagtg cgccgccggg caaactgttc
    15181 acgaactact ggagcaactc gaccaacgac aagcgggcgc ggctctggcg attaatcatc
    15241 aaatcgtccc gcgtgagcag tggacgcaac atatcgtgca ggatggcgac cagatcctgc
    15301 tttttcaggt tattgcaggg ggttgagatg ttacgtattg cggacaaaac gtttgattca
    15361 catctgttta ccggcaccgg gaaattcgct tcttcacaac tgatggtgga ggcgatccgc
    15421 gcttccggca gccagctggt gacactggcg atgaaacgtg tcgacttgcg ccagcacaac
    15481 gacgctatcc tcgaaccgct tatcgcggcg ggtgtgaccc tgctgccaaa tacatccggg
    15541 gcgaaaacag cggaagaagc cattttcgcc gcccatctgg ctcgtgaagc gttaggcaca
    15601 aactggttaa aattagagat tcaccctgac gcccgctggc tgttgcccga tcccatcgaa
    15661 accctgaaag ccgccgaaac gctggtacaa cagggatttg tcgtgctgcc ttactgcggg
    15721 gccgatccgg tattgtgtaa acgtctggaa gaagtcggct gtgcagcggt gatgccgctc
    15781 ggcgcgccga ttggctcgaa tcaggggctg gaaacccgcg ccatgctgga gattattatc
    15841 cagcaggcca cagttccggt ggttgtcgat gctggcatcg gcgttcccag ccatgccgcg
    15901 caggcgctgg aaatgggggc cgacgcggtg ttagtgaata cggcgattgc cgtcgcggac
    15961 gatcccgtca acatggcgaa ggcatttcgt ctggcagtag aagcaggcct gctggcacgt
    16021 cagtccggac cgggcagccg cagtcatttt gctcatgcca ccagcccgct gaccggattt
    16081 ctggaggcat cggcatgaaa accttcagca atcgctggcg acaactggac tgggacgaca
    16141 tccgcctgcg tatcaacggc aaaacggctg ctgacgtaga gcgggcgcta aatgcctcgc
    16201 aactcacccg cgacgacatg atggcgctgt tatcgcctgc cgccagtggc tatctggaac
    16261 aactggccca acgggcacag cgtctgaccc gtcagcgatt tggcaacaca gttagtttct
    16321 acgtcccgct ttatctttcc aatctttgcg ctaacgactg cacgtactgc ggattttcca
    16381 tgagtaatcg catcaagcgc aaaacgctgg atgaagcgga tattgccagg gaaagcgccg
    16441 ctatacggga gatgggcttt gaacatctgc tattagtcac tggtgaacat caggcgaaag
    16501 tggggatgga ttactttcgt cgtcatctcc ctgccctgcg tgaacagttc tcttcactac
    16561 agatggaagt gcaaccgctg gcggagacgg aatacgccga gttaaagcaa cttggtctgg
    16621 atggggtgat ggtttatcag gagacatatc acgaggcgac atatgcccgc catcatctga
    16681 aaggcaaaaa acaggacttc ttctggcggc tggaaacgcc ggatcggctg ggccgtgcgg
    16741 ggattgataa gataggcctc ggcgcgctaa ttggcctttc cgacagctgg cgcgttgact
    16801 gctatatggt tgcagaacat ttgctatggc tgcaacagca ttactggcaa agccgttact
    16861 ctgtctcctt tccgcgcctg cgcccgtgta ctggcggcat tgagcctgcg tcgattatgg
    16921 atgaacgcca gttagtgcaa accatctgcg ccttccgact gcttgcaccg gagattgaac
    16981 tgtcactctc cacgcgggaa tcaccgtggt ttcgcgatcg cgttattccg ctggcgatca
    17041 ataacgtcag cgccttctcg aaaacgcagc caggtggcta tgctgataat caccccgagt
    17101 tggaacagtt ctcaccgcac gacgatcgca gaccggaagc ggttgctgca gcgttaaccg
    17161 ctcagggttt gcagccggta tggaaagact gggacagcta tctgggacgc gcctcgcaaa
    17221 gactatgaca cggaatgtaa gaacgtaaat ttattccgag ccgccacgga agatgaaaaa
    17281 cgcaaggttg ttgaaagcgt tgcgtttttt tatggtagtg ccgtcagcag gaatgttgtc
    17341 cagggcgaaa agcttcttcc tcgtttcgcc ctgcctttct taaaaattcc gtaatgcaaa
    17401 gtctgaagcc atcgcctgtt gagcaatgaa agagatctga ttgtaagaga gtaaatactc
    17461 aactatgata gagacgaaaa taagaacaca tgttctcatc ttccaggatg cagcagactg
    17521 aagaaattca gacatcccgc aacctgcgat tatcgcaagg tcaaggcaaa gtccggtaat
    17581 ggcgttctga ataccagaga taattctctg gcgaaaccca ccttaaggtg ggttttgtta
    17641 ttttgagggc tgaggaagct gcttatttct caataagttg taggcaatgg tgaaaaattt
    17701 cactgattta cctttctgtt tttcagaaat gggataagcg ctagttacat gcaaacgaag
    17761 ttttttcttt tccctgaaag ccctgaacgc aacaaagtaa aatgccttgt ctcctccgtc
    17821 taagtccacc tcaatcacgg catagctacc atacccggca tgaataacgt tgctttctgg
    17881 cagcgccaaa atagttcttt ttaagtgtgt gcgcgctaag ttataacggt gctggcagaa
    17941 gggacgagat tctttaggcg cgtggtacat taacgattgt ttttcttcgt tcgtctgatg
    18001 ttcataatct tttgtgaagc agtgaagaga gtaggtaacc caaaagcgat actctaaagg
    18061 ctctttgccc tcatgatgct gaacgtagac agcctgatga gcatcaagaa atgataaatc
    18121 acgatatcac catccggatg tccaaaaggt cgccactttt ccacttcctg tttcattctc
    18181 gttccttaag aaagttacac aggaatagta tcgaatcctt gccgagtttg actcaattag
    18241 ttcagtcagt ttcaggatat tagtcatctc tacattgatt atgagtatcc agaaattcat
    18301 taaatattct gacaaatgct ctttccctaa actcccccca taaaaaaacc cgccgaagcg
    18361 ggtttttacg ttatttgcgg attaacgatt actcgttatc agaaccgccc agacctgcgt
    18421 tcagcagttc tgccaggctg gcagatgcgt cttctgcagt cacctgcggt gcagccggag
    18481 cttcacccgc agcacggcga cgcatacgat cctggtggta cgcgtaaccg gtacctgccg
    18541 ggatcagacg acccacgata acgttctctt tcaggccgcg cagttcgtcg cgtttgcccg
    18601 caacggctgc ttcggtcagc acgcgagtgg tctcctggaa cgatgccgcg gagatgaagg
    18661 actcggttgc cagagacgct ttggtgatac ccagcagatc gcgggagtaa gttgcaccca
    18721 ctttgccgtt cgcttccagt tcgcggtttg cgatcttgac gcgagagtat tcaacctgtt
    18781 cgccttccag gaagtcggag ctgcccgcat taacgatggt agctttacgc agcatctgac
    18841 gaacgataac ttcgatgtgt ttatcgttaa tcttaacgcc ctgcagacgg tatacgtcct
    18901 gtacttcgtt aacgatgtaa cgagtcacag catgaacacc acgcagacgc agaatgtcgt
    18961 gcggcgcttc cggaccgtcg gaaattacgt caccacgttc tacacgttca ccttcgaaca
    19021 cgttgagctg acgccatttc ggaatcatct cttcgtacgg atcgctaccg tctaccgggg
    19081 tgataaccag acgacgttta cctttggttt ctttaccgaa ggaaacgata ccgctgattt
    19141 cagccaggat tgccggctct ttcggacgac gtgcttcgaa caggtccgca acgcgtggca
    19201 gaccaccggt gatgtccttg gtaccgccgg attcctgcgg aatacgcgcc agggtgtcac
    19261 cagagctgat ctgtacgcca tcttccagct gaacaatcgc tttacccggc aggaagtact
    19321 gcgcaggcat atcggtacct gggatcagaa cgtcgttacc ctgagcatca acgattttca
    19381 gtgccggacg cagatcttta ccacctgcgg tacgttctgc ggaatccaga accaccagcg
    19441 aagacagacc ggtcagttcg tcggtctgac gagtaatggt ctggccgtcg atcatgtcag
    19501 taaagcgtac aaaaccgctt acttcggtga taaccggcat ggtgtgcggg tcccagtttg
    19561 caacggtttc gccgccagca acctgttcgc catcgccttt cgccagtacc gcaccgtaag
    19621 gtactttgta gctttcttta gtacgaccga attcgtcgat cagtttcagt tcggtgttac
    19681 gggaagtgat aaccagttta ccgctggagt tcacaaccga cttcacgttg ctgagcttga
    19741 tgctaccttt gtttttcacc tggatgctgg attcagcagc cgcacgagat gccgcaccac
    19801 cgatgtggaa cgtacgcatg gtcagctgtg tacccggttc accgatggac tgtgccgcga
    19861 taacaccgat tgcttcaccc ttgttgatga tgtggccacg cgccaggtca cgaccgtagc
    19921 agtgcgcaca tacaccaaag tcggtgtcac aagatacaac agaacgtact ttaaccgcgt
    19981 cgacagagtt ctcttccagc aggtcacacc actgttcgtg cagcagcgtg ttgcgcggaa
    20041 cgaggatatc agcagtaccc ggcttcagaa cgtcttcagc agttacacga cccagtacgc
    20101 gatcgcgcag cggctcttta acgtcaccac cctcgataac cggagtcatc atgatacctt
    20161 catgggtacc acaatcgtct tcggtaacca ccaggtcctg cgccacgtca accagacgac
    20221 gagtcaggta accggagttc gcagttttca gtgcggtatc cgccagacct ttacgagcac
    20281 cgtgggtgga gatgaagtac tggagtacgt tcagaccttc acggaagttc gcggtgattg
    20341 gcgtttcgat gatggagcca tccggcttcg ccatcagacc acgcatacca gcaagctgac
    20401 gaatctgtgc cgcagaacca cgcgcaccgg agtcggccat catgtagatg ctgttgaagg
    20461 aaacctgctt ctcttcctga ccgtcacggt taataacggt ttcagtttgc aggttatcca
    20521 tcatcgcttt ggatacacga tcgttcgccg cagcccagat atcgataact ttgttgtagc
    20581 gttcgcccgc agttaccaga ccagactgga actgctcctg aatttcagca acttctgctt
    20641 ctgcctcgga gatgatttcg tgtttcttct ccgggatgac catgtcatcg ataccaacag
    20701 atgcaccaga acgcgctgca tatgcaaagc cggtgtacat gatctggtcc gcaaaaataa
    20761 cggtcggttt cagaccgaga atgcggtagc aggtgttcag cattttggag attgcttttt
    20821 tacccagcgc ctggttgacg atggagtaag gcagaccttt cggtacaatc atccacagaa
    20881 tggcacggcc aacagtcgtg tctttcaggc tggttttcgc tactaattca ccgttagcat
    20941 ctttttcata ctcggtgata cgcactttaa cgcgcgcatg cagagaagcc agaccagagc
    21001 gatacagacg ttctgcttct ttcgggccag tcagcaccat gccttcgcct ttggcgttaa
    21061 cacagtcacg ggtcatgtag tacagaccca gtacaacgtc ctgagacgga acgatgattg
    21121 gttcgccgtt cgccggggac aggatgttgt tggtagacat catcagcgca cgcgcttcca
    21181 gctgggcttc cagcgtcagc ggtacgtgaa cagccatctg gtcaccatcg aagtcggcgt
    21241 tatatgccgc acaaaccagc gggtgcagct ggatagcttt accttcgatc agtaccggtt
    21301 caaatgcctg gatacccaga cggtgcagag tcggtgcacg gttcagcagt accgggtgtt
    21361 cgcggataac ttcgtccagg atatcccaaa cgacagcttc ttcgcgctca accattttct
    21421 tcgcagcttt aatggtggta gcaagaccac gcagttcaag cttgccgtag atgaacggtt
    21481 tgaacagctc cagcgccatt ttcttcggca gaccgcactg atgcagacgc aggtatggac
    21541 ctacggtgat tacagaacga ccggagtagt caacacgctt accgagcagg ttctgacgga
    21601 aacgaccctg tttacctttg atcatgtcgg ccaaagattt cagaggacgc ttgttagaac
    21661 cggtgatcgc acgaccgcga cgaccgttat ccagcagggc gtctaccgct tcctgcagca
    21721 tacgtttttc gttacgtacg atgatgtccg gcgcagccag atccagcaga cgtttcagac
    21781 ggttgttacg gttaatgacg cgacgataca gatcgttcag gtcagaagtc gcgaaacgac
    21841 caccatccag cggaaccagc ggacgcagat ctggcggcag taccggcaga acggtcagga
    21901 tcatccactc tggtttgtta ccagactgaa cgaacgcttc cagcagtttg atacgcttgg
    21961 tcagcttttt acgcttagtt tcggagttgg tttcgttcag ctcttcacgc agctgttcgc
    22021 actcttgctc cagatccatg ctcttcagca gagcctggat tgcttccgca cccattttcg
    22081 cgtcgaattc gtcaccgaac tcttccagcg cgtccagata ctgctcttca gtcaggatct
    22141 gctgacgttc caggttggtc ataccgcctt cgataaccac ataggattcg aagtacagta
    22201 cgcgttcaat atcgcgcagc ggcatatcga gcagcaggcc gatacgggac ggcagcgatt
    22261 tcaggaacca gatgtgcgca gtcggggaag ccagttcgat gtggcccata cgctcacggc
    22321 gtactttagt ctgggtcact tcaacgccac acttctcaca gatgacgcca cggtgtttca
    22381 ggcgcttgta cttaccgcac aggcactcgt aatcttttac cggcccaaag atacgggcgc
    22441 agaaaaggcc gtcacgttct ggtttgaacg tacggtagtt gatggtttcc ggctttttaa
    22501 cttcaccgaa agaccatgaa cggatcatgt ctggcgaagc cagagcaatt ttgatcgcat
    22561 caaactcttc ggttttagtc tgcgctttca gaaactttaa taaatctttc acggatttgc
    22621 tcccgtcgga gttagcacaa tccgctgccg ggttttaccc cgacagcagt gacctgtttg
    22681 agcgagaatt actcgtcttc cagttcgatg ttgataccca gcgaacgaat ctctttcaac
    22741 aatacgttga aggattctgg catgcccggc tccatctgat ggttgccgtc cacgatgttt
    22801 ttatacatct tagtacgacc attcacgtca tcagacttaa cggtgagcat ttcctgcagg
    22861 gtgtatgctg cgccgtatgc ttccagcgcc cacacttcca tctccccgaa acgctgacca
    22921 ccgaactgtg ccttaccacc cagcggctgc tgagtaacca ggctgtaaga accggtggaa
    22981 cgcgcgtgca tcttgtcgtc gaccaggtgg ttcagtttca gcatgtacat gtaaccaacg
    23041 gttaccggac gctcgaactg ttcaccagtg cgaccatcgt acaggcggat ctgaccggaa
    23101 gtcggcaggt cgccaagttt cagcagctct ttaatttctg cttctttcgc accgtcgaac
    23161 accggcgttg cgattggcat acctttgcgc aggttttcag ccagacgcat aacttcttca
    23221 tcgctgaagg tactcaggtc aactttctga cgaacgtcag cgcccagatc gtacgcacgc
    23281 tggatgaatt cgcgcagttt cgcgacttct tgctgctgtt tcagcatggc gttgatcttg
    23341 tcgccgatac ctttcgcagc catacccagg tgggtttcga ggatctgacc gatgttcata
    23401 cgagacggta cgcccagcgg gttcagtacg atgtctatcg gcgtaccgtt ttcatcgtaa
    23461 ggcatatctt cgatcgggtt gatcttagaa attacaccct tgttaccgtg acgacctgcc
    23521 atcttgtcac caggctggat acggcgttta accgccagat ataccttaac aatcttcagc
    23581 acgcccggtg ccagatcgtc gccctgggtg attttgcggc gtttcgcttc gagtttcttc
    23641 tcgaactcgt gtttcagttc gtcatactgc tcagccagct gttccagctg attttgtttc
    23701 tcttcgtcgg tcaggcccag ctccagccag cgatcgcgcg gcagtttgtc gagcttctca
    23761 gcttcaacgc caccggctac cagcacagca cggatacggc tgaacagacc cgcttcgagg
    23821 atctgcagtt cttcagacag gtctttcttc gcctgtttga gctgcatttc ttcgatttcc
    23881 agcgcacgtt tgtctttttc tacgccatcg cgagtaaaga cctgaacgtc gataaccgta
    23941 ccggatacac cgtttggtac gcgcagagaa gagtctttaa cgtcagacgc tttctcaccg
    24001 aagatcgcac gcagcagttt ttcttctggg gtcagctgag tttcaccttt cggcgtaacc
    24061 ttaccaacca gaatgtcgcc accggtcact tccgcaccaa tataaacgat accggattca
    24121 tccagtttgg agagcgcagc ttcacccacg ttcgggatgt cagcggtgat ctcttccggc
    24181 cccagcttgg tgtcacggga cacacacgcc agttcctgaa tgtggatggt ggtgaaacgg
    24241 tcttcctgaa caacacgctc ggatacgagg atggagtctt cgaagttgta accattccac
    24301 ggcatgaacg ctacgcgcat gttctgacca agcgccagtt caccgaggtc ggtggacgga
    24361 ccgtctgcca gcacgtcgcc acgttcaacc ggttcaccca gagacacaca cggcatctgg
    24421 ttgatacagg tgttctggtt agaacgggtg tatttggtca ggttgtagat gtcgatacct
    24481 gcttcacccg gatacatctc gtcttcgtta actttgataa cgatacggga agcatccacg
    24541 tactgaacga caccaccacg tttagcaacc gcagttacac cggagtcaac ggcaacagca
    24601 cgttccatac cagtaccaac cagcggctta tcagcacgca gagtcggaac ggcctgacgt
    24661 tgcatgttcg cacccatcaa tgcacggttg gcgtcatcgt gttccaggaa cgggatcagg
    24721 gacgcaccga cggataccac ctgctgggtg gatacgtcca tgtagtcaac ctggtcacgg
    24781 ctgaacaagc tggattcgcc tttgctacgg caggttacca ggtcttctac gaagtggcct
    24841 tcttcatcca ggttggagtt cgcctgggcg ataacgtagt tgccttcttc gatagcagac
    24901 aggtagtgaa tttcgtcagt tacaacaccg tcggtcactt tacgatacgg agtctcaagg
    24961 aagccgtatt cgttagtctg tgcgtacacg gacagagagt tgatcagacc gatgttcgga
    25021 ccttcagggg tttcgattgg acatacgcga ccgtagtgag tcgggtgtac gtctcgaact
    25081 tcgaagcctg cacgttcacg ggtcagaccg cctgggccga gtgcggagat acgacgtttg
    25141 tgcgtaatct cagacagcgg gttgttctgg tccataaact gagacagctg gctggaaccg
    25201 aagaactctt tcactgctgc ggaaatcggc ttggcgttga tcatatcctg aggcatcagg
    25261 gtatccagat cgcccagaga cagacgctct ttcaccgcac gctctacacg taccaggcca
    25321 acgcggaact ggttttccgc catttcgcca acggaacgga tacgacggtt gccgaggtgg
    25381 tcgatatcat cgacttcgcc tttaccgtta cggatatcga tgagcttttt cataacatca
    25441 atgatgtcgt ctttgctcag gataccagaa ccttcgattt cttcgcgcag cagagaacgg
    25501 ttgaacttca tacgaccaac cgcagacagg tcataacggt cttcggagaa gaacaggttc
    25561 tcgaacaggc tttccgctgc ttcacgagtc ggcggctcgc cagggcgcat catgcggtag
    25621 atttctacca gtgcgctcag acggtcgtta gttgggtcga cacgtaaggt ttcagagata
    25681 tatgggccgt gatccagatc attggtgaac agcgtttcga tacgcttgtg accagactgg
    25741 ctcagcttag ccagcagatc caggctcagc tccatgttcg ctgcgcagat cagctcgccg
    25801 gtagactcat caatatagtc tttagcaacc actttacctg cgatgtactc aaccgggact
    25861 tcgatcagtt tgacgtcgtc tttttccagc tggcgaatgt ggcgcgcagt gatacggcgg
    25921 cctttttcta cgtacacttt accgttagct tcgatgtcga aagatgcggt ttcaccacgc
    25981 aggcgttccg gcaccagttc catctgcagc ttgttatcac ggatttcaaa gataactttt
    26041 tcaaagaaca ggtcgaggat ctgctctgtg gtgtagttca gggcacgcag aatgatggtc
    26101 gcaggcagtt tacggcgacg gtcgatacgt acgaacaggt tgtccttcgg atcgaattcg
    26161 aagtccagcc aggaaccacg gtaagggatg atgcgcgcgt tatacagcac tttacccgaa
    26221 gagtgggttt tacctttgtc ggagtcaaag aagacgcccg gactacggtg cagctgggaa
    26281 acgataacac gctcagtacc gttgataaca aaggtaccgt tgtctgtcat gagcggaatt
    26341 tcgcccatgt agacttcttg ttctttaatg tcttttacgg tgccttccgg cgcttcgcgc
    26401 tcatagatca ccagacgcag tttaacgcgc agcggtgcgg aataggtcac gccacggatt
    26461 tgacattcct ggacgtcaaa caccggttcg ccaaggcggt agctgacgta ttgcagctcg
    26521 gaattaccgc tgtagctctg aatcgggaat acggaacgga aagcagcttc cagaccatac
    26581 tgcccttcag gatcttgctc gataaatttc tgaaacgagt caagctggat agaaaggaga
    26641 taaggtacat ccagaacttg tggacgttta ccaaaatcct tacgaatacg ttttttctcg
    26701 gtataggagt aaaccatagg gttcctcagc tcgctgacaa gtcgacccat ctgtccattg
    26761 agcggacagt ttgtgcaaca ctattttgtt gaccggaaaa tggaacactt tccgcaatgc
    26821 ctgttgctat cacgcttaaa ccatttcatt gcgatttaca cagaacggac gtcctgtcgc
    26881 agtatattaa gtcgtcgata gaaacaagca ttgaaaggca cagcagtagt caaacagtgt
    26941 gaaacgctac tggcgcctta cagcgcaaaa aggctggtga ctaaaaagtc accagccatc
    27001 agcctgattt ctcaggctgc aaccggaagg gttggcttat ttaacttcaa cttcagcgcc
    27061 agcttcttcc agagcttttt tcagtgcttc tgcgtcgtct ttgctcacgc cttctttcag
    27121 agcagccggt gcagattcta ccaggtcttt agcttctttc agacccaggc cagttgcgcc
    27181 acgtactgct ttgataacag caactttgtt agcgccagca gctttcagaa ttacgtcgaa
    27241 ttcagttttt tcttcagcag cttcaaccgg gccagcagct acagctacag cagcagcagc
    27301 ggaaacaccg aatttttctt ccattgcaga gatcagttct acaacgtcca ttacagacat
    27361 agctgcaact gcttcaatga tttgatcttt agtgatagac atttaaattg ttcctgaata
    27421 tcagaataag tttatacgta agcgaatgcg ttaaaaagat aactgcgatt aagcagcttc
    27481 tttcgcatcg cgtacagcag ccagagtacg aaccagtttg ccagccgaag cttctttcat
    27541 ggttgccatc aggcgtgcaa ttgcttcttc gtaggtcggc agagttgcca ggcggtcgat
    27601 ctgagacgcc gggatcagct caccttcaaa ggcagcggct ttgacctcaa attttgcatt
    27661 cgctttcgcg aactctttga acagacgagc agcagcgccc gggtgttcca tagagtatgc
    27721 aatcagggtc ggaccaacaa acgcgtcttt caggcactcg aacggagtac cttcaacagc
    27781 gcggcgcagc agggtgttac gaacaacacg catgtatacg ccagcttcgc gacctgcttt
    27841 acgcagttca gtcattttat ctacagttac gccacgggaa tccgcaacta ctgcagacag
    27901 cgcgcctttg gctacttcgc tgacttcagc aacaatcgct tgtttgtctt gaagatttaa
    27961 agccattagc tttgctcctg gatgtttgcc agagaaaatc tctggaactc acttcactcc
    28021 tccaaacgga gagagcgtct taattacggt gagcagaaac aagccagaat ataaaagaat
    28081 aatcttagcg ttctgtcacc gtctacgcag gggattaagt ttcttgtgaa actcctgcgg
    28141 tcttcgacgg aggcctggat aggccaggct ccaacgaaca aatcttttta gctgctaact
    28201 ttcgttagca tacgtggggg taagattgta gacaaaatca ccgcccacgt aaaggcatta
    28261 gtttacagaa gcgctcaggc cagcctggtc aactgcaaca cctgcaccca tggtggtgga
    28321 gatgctaact ttcttgatgt acacgccttt cgcctgagtc ggttttgctt ttttcagcgc
    28381 aaccagcaga gcttccaggt tttctttcag tttgtcagcg tcaaagtcca ctttaccgat
    28441 ggtggtgtgg atgatgccgt ttttgtcgtt acggtaacga acctggccag ctttagcgtt
    28501 tttaaccgct tcagcaacgt tcggcgttac agtacccact ttcgggtttg gcatcaggcc
    28561 gcgcggaccc agaacctggc ccagctggcc aacaacgcgc attgcatccg gagaagcaat
    28621 aacaacgtca aagttcattt cgcctttctt gatctggtca gccagatctt ccatacctac
    28681 cagttcagca cctgcagctt tagcagcttc agcgtttgca ccctgggtaa atacggctac
    28741 gcgaacggaa cggccagtac cgtgcggcag tacagttgca ccacgtacgt tctggtcaga
    28801 tttacgagcg tcgatgccga ggttaacagc aacgtccacg ctttctacga atttagcagt
    28861 cgccagctct ttcagcagag cgatagcttc gttgatgtcg tactgtttag ttgcatcaac
    28921 tttctcgcgg ataacacgca tgcgcttggt cagtttagcc atttcttagt cctccactac
    28981 caggcccatg gaacgtgcag taccttcgat ggagcgagtc atcgcttcaa tgtcggcacc
    29041 agtcatgtcg gcagctttgg tctgcgcgat ttcctgcagc tgagcgcggg aaattttacc
    29101 cactttgtct ttgttcggct taccggaacc agacttgata ccagccgctt ttttcagcag
    29161 aactgctgcc ggcggagtct tggtaacgaa agtgaaagaa cggtcagcgt aaacggtgat
    29221 tactaccgga atcggcagac ctttttcgat ggaatcagtt tttgcgttga acgctttgca
    29281 gaattccatg atgtttacgc cctgctgacc cagagccgga cctactggcg gactcgggtt
    29341 agccatacca gctgcaacct gcagcttgac ataggcttgt actttcttag ccattataaa
    29401 ttcctcaagt tgggtaataa cgcctctgag aggctccccg tgatataaaa aatcgtttta
    29461 cgggcaaggc ccataaaaac aaaaggcgcg aaattgtatt ccaatctcgc gccttgtgca
    29521 acgattaaat cgccgctttt ttgatcgctg ggttaggctt tttcaacctg gctgaagtcc
    29581 agctctaccg gggtcgcacg accgaagata gaaacagaca ctttcagacg agatttctcg
    29641 taatccactt cttcaacaac accgttgaag tcagcgaacg gaccatcatt aacacggacc
    29701 atttcacccg gttcaaacag cgttttcgga cgcggcttat caccaacctg ctgcaggcgg
    29761 ttcataatcg catcgacttc tttatcgctg attggcgcag gacgatcgga agtaccgccg
    29821 atgaagccca tcacacgcgg tacgctgcgc accaggtgcc agctcgcgtc gttcatcacc
    29881 atctgaacga ggacgtagcc agggaagaat ttacgttcgc ttttgcgacg ctgaccgcca
    29941 cggatttcaa ccacttcttc ggttggtacc atgacttcac caaacaaatc ttccatgttg
    30001 tgtaatttga tatgctcacg cagcgacgtt gctacgcggc cttcaaaacc ggaaaacgcc
    30061 tgaacgacgt accagcgctt tttaggagct tcagacatct cagaacctca ggccagtgat
    30121 aaaggatacc aggcgaacca gaataccatc cagtccccac aggatcagtg acattactgc
    30181 ggtaaccgca gccacaatca gcgtggtgtg caatgtttcc tggcgagtcg gccaaatgac
    30241 cttacggact tcggtacgcg cttcacgggc aaaagcaacg gtagctttac cttttgtcgt
    30301 taacagcgcg acaccacccg ctgcagcaat cagaattact acggccagcg cacgcagcgg
    30361 cagcataatg tcgcgataaa ggtagttgcc gacaatcgcc accaggagca atgccaccac
    30421 aacgacccac ttcatcgctt ccaggccgcg cccgcttcct tgagcttcgg tattcgcact
    30481 cataaaccaa cctgtcagaa gtattctaca aacattttca ccccgcgatc gcgaggcaaa
    30541 ccaaatcgaa acgcgtatta gcttttcgga ttatacgccc tcaacagagc ctgtctcagc
    30601 aatgattatg acaaagaaaa tcactgatga gccaggttct ggtacgaaag cgtgcaaaaa
    30661 gggcatcaaa tgatgccctt ttagtgcgca ttgcgtcaaa tgttatcggc aattagctca
    30721 gaacttttgc tacaacgccc gcgccaacgg tacggccgcc ttcacggatt gcgaaacgca
    30781 gaccgtcgtc catcgcgatc gggtggatca gggtaacaac cattttgatg ttgtcgcccg
    30841 gcattaccat ctctacgcct tccggcagtt cgatggtacc agtcacgtca gtagtacgga
    30901 agtagaactg cggacggtag cctttgaaga acggagtatg acggccgcct tcatctttgg
    30961 acagaatgta cacttcagat tcgaacttgg tgtgcggctt gatggtgccc ggcttagcca
    31021 gtacctgacc acgttcgatt tcttcacgtt tgataccacg cagcagaaca cctacgttct
    31081 caccagcacg gccttcgtcc agcagtttgc ggaacatttc aacgccagta caggtagact
    31141 tctgagtctc tttgatacca acgatttcaa cttcttcacc aactttgatg ataccgcgtt
    31201 ctacacgacc ggtaacaacg gtaccacgac cggagatgga gaatacgtct tcgatcgg
//
LOCUS       DAZGBC010000042        31140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGBC010000042 DAZGBC010000000
VERSION     DAZGBC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.042"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(226..1167)
                     /gene="fabY"
                     /locus_tag="ACHK1J_001926"
                     /gene_synonym="yiiD"
     CDS             complement(226..1167)
                     /gene="fabY"
                     /locus_tag="ACHK1J_001926"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO7911200.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1212..1649)
                     /gene="dtd"
                     /locus_tag="ACHK1J_001927"
     CDS             complement(1212..1649)
                     /gene="dtd"
                     /locus_tag="ACHK1J_001927"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO7911201.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1646..2518)
                     /gene="yihY"
                     /locus_tag="ACHK1J_001928"
     CDS             complement(1646..2518)
                     /gene="yihY"
                     /locus_tag="ACHK1J_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO7911202.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2512..3111)
                     /gene="yihX"
                     /locus_tag="ACHK1J_001929"
     CDS             complement(2512..3111)
                     /gene="yihX"
                     /locus_tag="ACHK1J_001929"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO7911203.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TAFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3252..4139)
                     /locus_tag="ACHK1J_001930"
     CDS             complement(3252..4139)
                     /locus_tag="ACHK1J_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO7911204.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4129..5478)
                     /locus_tag="ACHK1J_001931"
     CDS             complement(4129..5478)
                     /locus_tag="ACHK1J_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911205.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(5577..7010)
                     /locus_tag="ACHK1J_001932"
     CDS             complement(5577..7010)
                     /locus_tag="ACHK1J_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911206.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNTLCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLIHWEPLGANVDAEDAEVLAELKK"
     gene            complement(7080..8090)
                     /locus_tag="ACHK1J_001933"
     CDS             complement(7080..8090)
                     /locus_tag="ACHK1J_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO7911207.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(8123..9010)
                     /locus_tag="ACHK1J_001934"
     CDS             complement(8123..9010)
                     /locus_tag="ACHK1J_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO7911208.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQIMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(9035..9913)
                     /gene="yihU"
                     /locus_tag="ACHK1J_001935"
     CDS             complement(9035..9913)
                     /gene="yihU"
                     /locus_tag="ACHK1J_001935"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO7911209.1"
                     /translation="MATIAFLGLGQMGSPMANNLLHKGHSLQVFDVNALAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            10086..10982
                     /locus_tag="ACHK1J_001936"
     CDS             10086..10982
                     /locus_tag="ACHK1J_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7911210.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIARLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            11022..11810
                     /locus_tag="ACHK1J_001937"
     CDS             11022..11810
                     /locus_tag="ACHK1J_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7911211.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(11921..13186)
                     /gene="yihN"
                     /locus_tag="ACHK1J_001938"
     CDS             complement(11921..13186)
                     /gene="yihN"
                     /locus_tag="ACHK1J_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911212.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVKLKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(13288..14268)
                     /gene="yihM"
                     /locus_tag="ACHK1J_001939"
     CDS             complement(13288..14268)
                     /gene="yihM"
                     /locus_tag="ACHK1J_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO7911213.1"
                     /translation="MMTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKKSIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(14276..14986)
                     /gene="yihL"
                     /locus_tag="ACHK1J_001940"
     CDS             complement(14276..14986)
                     /gene="yihL"
                     /locus_tag="ACHK1J_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7911214.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(15203..17026)
                     /gene="typA"
                     /locus_tag="ACHK1J_001941"
     CDS             complement(15203..17026)
                     /gene="typA"
                     /locus_tag="ACHK1J_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO7911215.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            17399..18808
                     /gene="glnA"
                     /locus_tag="ACHK1J_001942"
     CDS             17399..18808
                     /gene="glnA"
                     /locus_tag="ACHK1J_001942"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO7911216.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18982..20031
                     /gene="glnL"
                     /locus_tag="ACHK1J_001943"
     CDS             18982..20031
                     /gene="glnL"
                     /locus_tag="ACHK1J_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO7911217.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            20043..21452
                     /gene="glnG"
                     /locus_tag="ACHK1J_001944"
     CDS             20043..21452
                     /gene="glnG"
                     /locus_tag="ACHK1J_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO7911218.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            21564..21674
                     /gene="yshB"
                     /locus_tag="ACHK1J_001945"
     CDS             21564..21674
                     /gene="yshB"
                     /locus_tag="ACHK1J_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO7911219.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(21864..23237)
                     /gene="hemN"
                     /locus_tag="ACHK1J_001946"
     CDS             complement(21864..23237)
                     /gene="hemN"
                     /locus_tag="ACHK1J_001946"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO7911220.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(23426..23932)
                     /gene="yihI"
                     /locus_tag="ACHK1J_001947"
     CDS             complement(23426..23932)
                     /gene="yihI"
                     /locus_tag="ACHK1J_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO7911221.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(24051..24217)
                     /locus_tag="ACHK1J_001948"
                     /pseudo
     CDS             complement(24051..24217)
                     /locus_tag="ACHK1J_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24514..25146
                     /gene="yihA"
                     /locus_tag="ACHK1J_001949"
     CDS             24514..25146
                     /gene="yihA"
                     /locus_tag="ACHK1J_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO7911222.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(25528..28314)
                     /gene="polA"
                     /locus_tag="ACHK1J_001950"
     CDS             complement(25528..28314)
                     /gene="polA"
                     /locus_tag="ACHK1J_001950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO7911223.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIESGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALDEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAYEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LKYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            28678..29610
                     /gene="yihG"
                     /locus_tag="ACHK1J_001951"
     CDS             28678..29610
                     /gene="yihG"
                     /locus_tag="ACHK1J_001951"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO7911224.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRHDK"
     gene            complement(29651..31081)
                     /gene="yihF"
                     /locus_tag="ACHK1J_001952"
     CDS             complement(29651..31081)
                     /gene="yihF"
                     /locus_tag="ACHK1J_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7911225.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAS
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEMNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLHY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         7881 a         7981 c         7447 g         7831 t
ORIGIN      
        1 atcgctcaaa catgaactaa gatgatgaat taagcattat ctgcgataat tgccatacag
       61 ctctacaaat aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggagcca
      121 acgaagaata aaaatttata gagttcttat tcaatataac cgaaatagcc aaggactaat
      181 ataaatgacc tgaatgagag aggcagcacc gcccctctcc atcagctact cttcttcgtt
      241 cccgccctct tcatacgggc caaatggctt cgcgggcaga acgatatacg tgccttcaaa
      301 cactgcaccc ggcgtctcgt cgccaaagat ttcgacctgc atctgcaccc gtgcttttcg
      361 tccgcgcgcc agacggtcga gatcgccgct taaggcaccg aggtcggcta ccgcatgagg
      421 tttaccgcta atcggcttgc tgtagcggat atgcgcatcc gcaagaataa tcgttccgcc
      481 gaggtggcgt tcacgcagca tcagccagat aagtccccaa ccggtgagcg tcgccagtga
      541 gaataaactc ccggcaaaca gcgtatggtg cggattctga ttgccggttt ctggcatggt
      601 agtgataaat ttttgcccgg tatattgctg aatgcgcacg cccatttttt cactaagcgg
      661 gatatgttcg taccacgcct gttgcagctg cgcgcaccag tcgccgcgat gcagaatatc
      721 atccagagtg gcgacgggct taatcatcaa aaaatggcga atcggcgtgg tggttggcgt
      781 ggtgatttct ccctgattaa caaaccccag cttggcgaaa aactccaccg cgtcttcacg
      841 ggcgctacag gtcacgcgct taacgccttc ctgacgcgcc accgactcca gggtcatcgc
      901 catcagcgtg cctaaccctt tgtcctgcac gtcgggatga acggccataa agcgaatgga
      961 cgcttcattg tcggcattaa tatacagtcg gcctaccgcc accagattac cctgctcgtc
     1021 gacgaccatc tggtgatgcg ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg
     1081 atgcaggggc ttacgcaaca tttcccagcg aaactgatag taacgctcta attcttcttc
     1141 tgtttgtgga acccgaaggt gatacatagc gatactctct cttgtttccc gtgacaaccc
     1201 tggaagctgg ctcatacctg caaccagaat gtcacggggc catcattgac cagcgatacc
     1261 tgcatatccg cagcgaagcg tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg
     1321 acgaaatagt catataacgc ctctgcgcga tccggtgatg cacctttgga gaaacttgga
     1381 cgcatccccc gttcggtatc tgcggcgagg gtaaactggg aaaccaccag cacactgccg
     1441 cccgcctgtt gcacgttgag gttcatcttg ccttcggcat cgctaaagat gcgatagccg
     1501 agcacacgct cgcacagacg gtttgctttc tgttcgtcgt catccttttc gacacccaat
     1561 aacaccaaaa gtcccgcgcc aatttcgccc gtcacttctc cctccacggt gacgctggca
     1621 cgggttacgc gttgaattaa tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt
     1681 gttttagttt gcggtattcc ccgagagtga cagtaatttc cgcgccaagc aagacgatac
     1741 accatgtcca gtagacccaa acaaagagaa tggggatcac cgccagcaca ccgtaaatga
     1801 gctgatatga cgggaacatg gtgatataaa gcgcgaaacc cttctttcct gcttcgaaca
     1861 ggagcgcggc gacaaacgcg ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa
     1921 caatgctgta cagcaaccag aaggagatcc acgacaacag cagcggaaaa atacgcagca
     1981 cattgtcgat gacagtattg agatcgctcg cccagcgcag ggagagcaaa taggaactga
     2041 tcgccagact ggcccctgcc agcagcggcc ccagcgttaa aatcatccag tacacggcga
     2101 acgagtaaat ttttggtcga gctcgtttac tgcgccagat ggtattcaac gcgctatcga
     2161 tggagtacat caacaataac gcggtgacga tcagcccgca cgccccaacg gcggtcatct
     2221 tgttggaatt ggcaacaaat tgttcgatat accgctgaat aacatcgcca gtagcaggca
     2281 gaaagttggc aaaaataaag tgacgtaact gaatgctgac gtcggaaaac atgggaaaag
     2341 cggcgaaaag cgcaaaaaca acggcaacca gcggcactaa tgagagcaac gacacatagg
     2401 caaggttacc tgccagggtt gtcatattgt cctcatcaat gcgttgccag agtagtttta
     2461 gccaggccca tagtggacgg gtacggtgcc tggctttgtc ctgaatggtt tttagcataa
     2521 caccttcgcg aaatagtccg ggatggtggt tttatctttc accagaatac tggtaatgcc
     2581 tagctgatta gctccttcta tattatcggc gttatcgtcg aaaaaggccg tatcgctggg
     2641 tgaaaaacct tccgcctgca aaacatgctg gtaaattcga gcttcaggtt tgcgcatccc
     2701 cagatcttgc gacagataga tatggtcagc agcatcacga atttccgggt attcttccgg
     2761 ccagaaggtg gtatgcaggc ggttggtatt ggaaagcacc accacgcgat gcccctgctc
     2821 acgcagttta tgcatgatgg cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca
     2881 gccgtgagag aactgctcgt agcttagcgg tagagccatc tcatgacaca gcgcctctgc
     2941 gaacgcttcg tcgctaattt ccccacgctc atgctgatga aacgcctccc ccatatgaaa
     3001 actcttctta agcgtagcca gcggaatacg cgttaaatcg ctccaggctc ccagcacacg
     3061 gttaaagtcg atatcgacaa tcacattacc taaatcaaag atatagagca tttttgcctc
     3121 ctttcgcgcc acaagaaatt aactgtagcg ggaaaggatg ggtttggcta tgtggcaata
     3181 agggtaatct tgtgatgctg attgcgaaaa agcagagtaa tttcactggc tttttcgcaa
     3241 tagatgaatg gttaagattc aggtgtaaat tgccaacgag tttcgataac cagcggccta
     3301 tctggtgtca ccaattccgg cgttagattt attgcgttag gtgcctgagt cagcgggtta
     3361 atacaggtgg catcgggctg tttatcaaat accaccagac tgtttgcaga agagatcatg
     3421 gtcatcgcga gtttaccagg ccacagtaac ttaaccttca ccccgtcata aaaaccaaaa
     3481 cagtcatccc agggaccgac ttgaggagag atgcgatttc cagttggcag ttcatcacta
     3541 cccgcttctt cttgccagtc cgcatcaaaa agcacctgca acgattctgt attttgcgga
     3601 gtcagctttt tcgcaaacca cggatgccag cctgcggagg ccgggaatgt atcggcatat
     3661 gaatggactt ccagttgcaa aaccagcgca tcgttctcca gcaaaaaagt ctgaatcact
     3721 tccccttgcc aaggccacgg tgaagccagg ggcatacgta aagtcaggga atctgctgtt
     3781 ttgtcgatga tttcccacgt tgagtaacaa gccataccat gcaacgcatg tggtggttta
     3841 ttggcgggta aagaataaca ttgcccacca gcattcagcg tcgcattccc cagtcgccct
     3901 gcccacggca ccattgggaa acagccatac tgaaaagctc tacgctgcgg ctgccattgg
     3961 cgtaatactt cagaaccaaa tgcctttaac gaggttatcc tggcaccatc atgcggataa
     4021 atctgcaggt gaatattgcc tttttccagc gtcactaact ctcctacagg cccaggccat
     4081 ggcgcgtcaa acgcctgttg tagcgccagg taatgctcgt tttttcgttc agttttcatg
     4141 agattgttcc ctgatgtagc ggtaagaagc ggtttcttgt tcatcggtcg gctcaatcag
     4201 ccagacgcaa acaaaggaaa gaactgagac agcaaataaa aacagagcga cataccacgg
     4261 ttttcctccc cctagcgcca gcaaacttga ggcgataagg gggcttagtc cgccaagaat
     4321 cgcggaaaat tgataagcaa aagaaaggcc ggtatatcgt actttggtac cgaacatgcg
     4381 ggtgaaaagt gttggctgca cggcaaacat catcgtcgga cctaagttgt agccgaggat
     4441 catactgcac cagatgatga gcgtactttt gctatccagt aataagaaga aaggaaacgc
     4501 gaacaggaca caaaatcctg ctcccgacaa atacagactt ttttgaccta tacgatctga
     4561 taaccatcca accaatggac aactgaaaat accgatcgcc gaggcaatca acatacccgt
     4621 caggggaata tctcgcgata atgctaattg gctggaaata tagacaatac caaaggcgtt
     4681 aataatatta gaggagacac tttccgccag cctcgcaccc agtgccaata aaatatttcg
     4741 tggatgtttt ttgaataatt caatcaacgg aggaacggac ttttctttat tattcgtcgt
     4801 tttttgcttt tgaaaatcca gcgtttcttc ggtatgcagg cggataaaca gcccaacgat
     4861 caacatcacc gcagaaagcc aaaacggaat gcgccatccc caggagagaa actgctccga
     4921 agtaagaaaa tggttacaga gagcaaaaat accggttgcc agcataatac ccacagacgc
     4981 agccgtttgc gggagtgaac ctaagaatcc gcgtcgggat tcgggcgctg attctatggt
     5041 catcaatgcc gctccaccgt actcgccacc aagaccaaag ccctgaatca aacgcagcac
     5101 cattaataca agcggtgccc aaatgccaat ttcctgatac gtcggaataa aaccaatcaa
     5161 aaatgtggag caaccgacaa tagccagcgt ccagataagt gttatttttc gcccgatttt
     5221 atcgccaaaa tgaccaaaga cgatgccccc taaaggacga gtcagaaaac cgacaccaaa
     5281 ggtcgcaaag gaagctataa gacctataaa aggatcgctg tctggataaa acagcggtgc
     5341 aaataccagt cctgctgccg tgccaaaaat aaagaagtca taccattcca ttaacgcgcc
     5401 tgcgaaactg cccataacga cacgtctcat ggctttaccg gaaagccctt cacgcggtgc
     5461 gggtaacttt tcagacataa ttttcctctc ctggtaggcg agaaatctgg gatacgaacc
     5521 actaaacgtg ggcagtttga attaatgcgc atcggcttaa gccgatgcgc attaaattat
     5581 ttcttcaatt cagccagaac ctctgcgtcc tcagcatcaa catttgcacc cagtggttcc
     5641 caatgaatca atacgcaggt caggaacccc aggaatggca cgattgccaa ccagtaaaac
     5701 gcttcagttt tcaacgcacc ccacagcatc ggccagaaaa tgagtcctgc aattgcccct
     5761 gtgcgcataa tagcgcgggc aaatccgacc ccagtagggc gaatagcggg aggataagag
     5821 agtgtggcaa tggtcatgcc cagcccacca ggacccgctg aatggaagaa aataattgat
     5881 cccaataacc ctaaagacag ccagggatta gtcgttgcca gtgcgcctaa cgacagcaat
     5941 gctaaggtca caacagcaaa tccatacatc gactgtcggc gagtacccag tcgttggaga
     6001 ataaacgagc cgatcatccc gcctgtcacc ccacaaaggg tattcacgac ggcagaaccg
     6061 gtcaacgcgc cagtaagccc gccacttatt attcccgcca atgtaagagg aagataaaca
     6121 cctacggcgt tatattgcca cgcctgcatg gttgccacga cacagccaag aatggtgcgt
     6181 ttgcggtaac gatcattaaa taagatgcca tatccaccac tgtatttatt ctctgctttt
     6241 tccgagaatt tcgcttctgt tgtactcgat gccgcaacgc gagcctgaac accataacgt
     6301 tttcccagaa tgtcgcacgc ttcctggtaa cgcccgacgc gtgcggccca cattgcggat
     6361 tcaccaataa agaagtaacg caaaatcatg aagatgactg caaaaatagc tccgatgaat
     6421 attccgtaac gccacaattg ttcttcagcg atacccgaca gaagcagcgg caaaagtacc
     6481 aaataaacaa cggttgtcga aacataccag gccatttgcc agaggttgac cgacgttccg
     6541 gttttcttcg acgttgaacc acgaagctct gccacggcat tggttgccaa agggaagtcg
     6601 atgcctaaac caaagcccat gatgacgcgg cacaccagca cccaccagat actgggagca
     6661 atagctaccg ctgcagcacc gatggtgcac agccccatgc caataataaa tgcccgcttt
     6721 tgcccaaagc ggtttattac tcggttcgct aataaaccgc caattaacgc ggcgattaat
     6781 actgatgcat tgacggttgc agcaagaccc ggcgaaatgc cgaattgagc ggcaatgtat
     6841 ttattaccaa accccaccat cgccgcctgg taggcatcaa tcaatatgcc gcccaaagca
     6901 ataaaaataa cgccgatact ggtgttgatt gcagagttct tattaacaat atcaattacg
     6961 tcctgtggcc gactaattga ataagttgca ggactggtga tttgactcat cgcagtgtcc
     7021 tttttaattg ttgtagggtg gcgctgacgt gacgcagcgc cagacagaga tttagaaaat
     7081 cattccggcg caaattgcag catgaccttt atggagtcat tttgctgcgc gcgttcgaat
     7141 gccgccgctg catcctcaat ggcaaataaa tcggtaacca gttcactggt atcaaaccgc
     7201 ccatccgcca gccattcgat aacagcatcg aaatcttcag ggatgtacat gccagagttg
     7261 agcaagtcac gctcgaaacg ttgcatcatc ggtaatggta tttcacgcgg cccacttggt
     7321 acgcccatgc acacgacggt gcctccggca tatacacggg tacatgcatc gttaagcgtg
     7381 gcctgcgctg caacgacatc aatcgccccc gtaaatcgca catctgcggc cagctcacct
     7441 ggtgcccaaa cttctgctgc gcccagcttt aacgccagcg cgcgtttggc agcgtcaggt
     7501 tcaatcacgg taatcttacc tgcccccata atgcgcagtg cctgcacgat ggataagcct
     7561 atcgttccgg caccaatcac cagtacgctt tccagagaag ctttcggcat tcggttaacg
     7621 cagtgacggg cacaagccgc cggttcggca aaggccaaca cttttagcgg taaagaggct
     7681 ggtgcgacat ggcaattacg cgcaggaaca atgtgttgtg agcgagcaaa gcccggtgcg
     7741 cgaaaaccag caacctgcgg tggttcacaa agattaaaac gtcctgcttt gcaggctcgg
     7801 cattccatgc aagccatgat gggatcgacc acaacatgat cgcccggctg tacattttta
     7861 acgtcgctgc caacttccgt aacgcgcgct gcaatttcat gaccggggac gacaggtggt
     7921 ttggcaaacg gatgaccgcc tttcagaaca tgcagatccg aaccgcatat cccatagaac
     7981 acaggcgcaa tccgcacttc gtgcggttta agttcctggt gtggtacttc aacatattga
     8041 gtggtgactt tcccaatatc tgaaaataaa acctgggtaa ttttatccat tgttattctc
     8101 cagaatcgat atccataggg gattagcgtt gcgcgatacc ttcatcgaca atttctgcca
     8161 accgttgtaa acgaggaaca gagacatcgc gcagcataat ttgtggatct tgtgcaccaa
     8221 caaccgacgc ccaaaccgct cgtccagcca ggaagcctga cgcaccacct ttcatggcaa
     8281 tacttactgc acggccaaaa atatcggcat caacgccaga agaaagaatt acccacggca
     8341 tcttcatctg atcgttaagt tgctgacagg cggcaagtaa ggttttttca tcgcctttgc
     8401 cccctaatgg catctcggct ttgtaaagat cagcctctgt tccaccaagc tctgcggcgg
     8461 cggcaacaat ggcgctttca cgatcaaagt cccaaccgcg acgaggtgga cgcaccacag
     8521 gttcaataat gctaaccagc ccggcactac ggcagcgacg aacaaattta tcaaccattg
     8581 ccagacgttc ctctgcgggt tcatcttcac gccagagtac aagtaatttc attgccttag
     8641 cccccatttc acgggcttta tgcgggtcga cggacatatc tatttcaacg ctatcaaccg
     8701 ggatgccatt acctggaata aaaagatcgg cagcgacaat caggccgcaa gaattagcaa
     8761 cagcgccctg ctcgactatt tgctcaagac aaaattgttt atccgccaga acggcagaag
     8821 cataaggaga aaggattcgg gtcgcggcaa ctttaaaatc ggtgagtaca ctgtcggcga
     8881 tgggttttgg ctgccctgct gccgcaaaca tcaagcgcat ggcctcacgc tgatcgacag
     8941 ctaacatggc aaatccacca cctgggcggg agatatcttt cagcgtaaat ggggtgaagt
     9001 gggatgacat agaaactcct gttaatagat aaaattaaag accagcctgc tggcgaacct
     9061 gatttagcag ggcactccag tcctgccgcc ctttgccgtc ttgacttgct gcagcgtaat
     9121 aactttctgc tgcaatcccc atattgagcg gtgcccccgt ctcccgagcg acgtcaactg
     9181 caatacgcag atctttcaac gccagatcca ccatgaaggc gggtgaaaga tcgcctttca
     9241 gcactttgcc aggccaggtg gtcgtaaaat gtcctttccc tgctgccgta ccactcatca
     9301 ccttaattgc gacatcaaga tttaatccca gtgactcaca taacacagcg gcctcggagg
     9361 acagcgcatt aagcgcaatg ctcatatagt tgttaattag cttcacgcgg atccccattc
     9421 cagggccgcc agcctcgacc agctcattac ccatgcacat gagaatcggg cgtgcacgtg
     9481 caatctgctc atcagtgcca cccgctaaaa tcagcaatgt gccatcaatc gcattcacgg
     9541 acgttctgcc cacgggggcg tccatcatac tgatgccttt ttcttgtaac tcccgtatta
     9601 atgcatcggt ctgtaaggga tgaatagtcg acatatcaat caataacgcg tcagatgaaa
     9661 cagcctcaca gacgcctttt tcccccaaca gaacctgtcg aacaatgtca ccattgggaa
     9721 gcatagtgat gataaattca gcgcctgctg ctgcttccgc tggggtttgt gccgcagtgg
     9781 ccccctgcgt aactagcgca tcgacggcca gtgcattgac atcaaatacc tgcaatgaat
     9841 gccctttatg caatagattg ttagccattg ggctacccat ctgccccagc ccgagaaatg
     9901 caatggttgc catgaatact ctcctgtctg acattgacaa aataatgaca attgaaaatt
     9961 cgttttcgtc tgtttttgag catatttgta atttatgttc aaaaacacga catacatcac
    10021 taatttgatc atttgtgtca tttaaattga ccaaagacga caaaattaaa caggacaaga
    10081 caaatatgac cagaattgca tgcgtgggta tcacagttca ggacaggatt tattctttgc
    10141 ccactctgcc ggagggcggc gggaaatatc aggcaaatca ttatcttgag attggtggcg
    10201 gcccggcagc aacagccgct gttgcaatcg caaggcttgg cgtggaagtt gatttcatcg
    10261 gtcgcgtcgg cgatgacagt tgtggaaata ccttacttgc agaacttgag ggttggggcg
    10321 tcaatacagc attttgccgc cgatatccta acgcccgttc gtcgcaatca gcgatcctcg
    10381 ttgatcagca tggcgaacgg attatcgtta attatccaag tcctgatctc ggtactgatg
    10441 ccgaatggtt agaagcgatc gatttctctc gttatgatct gattctggcc gatgttcgct
    10501 ggcatagcgg tactgaaaaa gcattctcgc tagcgcgcct tgcgggtgtg acaacgcttt
    10561 tagacgccga tatgacaccg caggatattt ctccacttgt cgcgcttgcc gatcacgccg
    10621 tgttctcaac gccagggctg aaacgcatga ctggcctgca gtcacctgaa gaaggtttgt
    10681 tccaggcaac gacacaaaca gcaggtaaag tgtatgttac gctgggttct gaaggatcgt
    10741 tgtggattga agatggtcat ttgtgtcaac aggaggcatt ttctgtcaat gtggttgata
    10801 ccactggagc aggcgatgtt tttcacggcg cactcgcggt agcgttggca gaaaaaatgc
    10861 caacaaaaga ggctattcgt tttgccagtg cggttgccgc gatgaaatgt acccagcctg
    10921 gcggtcgggc cggcattcct aatcgtgagc aaaccgaatc atttttgtca ctttatgcgt
    10981 aaaatggtaa acatctaccg aggaggggca agggagtatt catgagtatc atcgaagtga
    11041 caggtaatcc acgccatgac caactcgttc atctaatcgc agaacgcggt tatatgaata
    11101 ttgaagaact ggcacaactg ctggatgttt caacgcaaac cgtgcgccgg gatatccgaa
    11161 aactcagtga gcagggcttg atcacccgtc atcatggagg ggccgggcgc gtctccagcg
    11221 tcatgaatac cgcttttgag caacgggaac tttcgctcac cgccgagaaa cgggcgatcg
    11281 ctgaggcagt cgccgattac cttcccgaac gctgtaccgt ctttatcacc atcggtacaa
    11341 ccgtagaagc cgttgccagg gcattactca accggcgtga tttacgcatt atcactaaca
    11401 gcctacgtgt ggcacagatt ctttataaga atcaggatat tgaagtgatg gtgccgggag
    11461 gcactttacg cgctcataac ggcgggatta tcggcccagg agccgtggat tttattgaag
    11521 gcttccgcgc agattattta atcaccagta taggtgccat tgaacatgac ggcaccctac
    11581 tggaatttga tgtaaatgaa gcgttagtcg cgagaacgat gattaaacat gcgcggaata
    11641 cattgttagt ggccgatcat acgaagtttg ccgcgtctgc tgccgtttca attggcaatg
    11701 cacggaatgt cagggctttt tttactgatg ccccgcctcc caattctttc tgccagttgt
    11761 taagtgaaga gaatgttgaa ctggtggttg ccgagcagga agtttcctga aattgaaact
    11821 tcgaaaaatc cgctggggat tcagcggatt ttattttgcc ggatgcggcc tacggttgat
    11881 acggttttcg ttggccggat taagcacatc cggccattaa ttaagcttca gccgccagca
    11941 tctctttctt acgttggcta atacgcttaa ccagcattac ggaaaccacc gcaccacaga
    12001 atgcgaagca ggccataatg ccaaacacga ttttgtagcc aataatcccc ggatttaaat
    12061 ccagaatgta gccatacaga ctgaagcaga acatcgccgg ggcgtaacca ataaagctac
    12121 ccaacgccat cgccgcgcct gttttatttt cagcaatttt tgcttcgccg ataggtgcaa
    12181 aaaatacggc tcgctgtgta aagactatcg cgccaaagcc cagcgtacat gccatcccta
    12241 aatagaccgg catactttcg tgaggtagca taatcaacag tacgagcgca gcggtactga
    12301 tgataaaggt gtagcataga tatttactcg gcgatttcag gatcttatct gaaatcatgc
    12361 caccaatcgg tccaccaatc attttcagac agtattggtt aatgatgccg taagccccca
    12421 ccagcgcaac gggcaataga tagatgtttt tcaggaatgg aatgaagaat gtcaggccac
    12481 agtaaaccgc gtaaacgaag aagacgttga aagcgataag ccagatagtt ttgtctttca
    12541 gcaccgaggt catactggtg ttttgcgatg ccccgtcttc ttttttcaac ttaacggacg
    12601 gtgcctcttc tttgtcattc aggacaaaga aaataataat acctacggca atcacaataa
    12661 gggaatagaa ccagatgcct gctttaaaac ccaataagcc actgccaaac caggtaaata
    12721 ctgccaacgc agaaaatgcc accacggtat cgacaatgcc acgccctgtt tcgaagaagc
    12781 caaacaaccg accttgttgt tcgctattgc ccaatcgact taccgacttg agcaagaccg
    12841 gccagttcat catgtcgcaa gtaacgccaa atagcgccca gacaaagagg atgccccaat
    12901 accccggcat tgtcgtcaaa taaacaccca gtaatcctgt cgcaatgagt gagaatgaca
    12961 tggtgtatct gcgcggtagt ttatcagcaa aataaataga cagaaaaaag cccactgtgg
    13021 taacaaatga gtttaccgac atagcattac caatttgacc attggtcaaa tggaaatatt
    13081 cctgcatcgg gatataaaac gcatctttca gcgacggtaa tttataaatt gtaccgccac
    13141 acagtgttaa tagactaaat aacgcccatt tctttttcgt gagcataaca tcccctaagc
    13201 gatttttaaa atcactgttt taaaggcaat aagataccga ccattcatat ggaatggttt
    13261 tattaactgc gtatgagtta aaacttatta gttgttcaga agatggtttg cctcttgttt
    13321 gatttgttgt agtacgttac gcacatgatt tatctgatta attgcatctt ctttttcttt
    13381 acgtaaccgc gcatccagta aatgattttc tggtaggggt gtttcactca tctggcgata
    13441 agggatccac ggattatcat cttcgtcttc aaagcggaac gccggcgcat agtaatcaac
    13501 ctcttcttcc aggccatatg ccgtcacctg cggctcatca tcacccaggc agataaggtg
    13561 cgttaataac gctttgaacg gcatatcacc ctgacctgaa atacaggctt tatgacccag
    13621 gccaccctgt tctttaacga tcaaggcatc tttaatatgg acctgggtaa tatgcggtgc
    13681 catcgttttt aaggcgtcga tgggatgctc atttgcattg atcatgttcg caaaatcaaa
    13741 cagtaaggaa agagactcca tctcactttc tttgaccagc gacaccaact catgactttt
    13801 taaatcttca tgctgctcaa tcgtaaaagt caggccgctg tcctgatacg tttctcgtac
    13861 ataggcaatg tcgttagcga taatcgataa tacgtcgcgc aaattacctt cataacgcgg
    13921 ataaaaacgt acggacgatg ccccagtttt caacgcgatg gcgacggctt cgtcaataga
    13981 ttttttatct gaggcgctgg tttcaatatg aatatcaagg ttcagacggc gggctttatc
    14041 cccaaaggca gagagttctt tatcgtccat attaccaaga gaaaaacgct cgccatccag
    14101 aacatggatt ttgacgccac gtagattatt ttcacttgcg atatcaagta aatcagcagg
    14161 caacactctt tccagccgca tatttaaatg aaaggcataa gcatgtaaat aaagaggaag
    14221 agtgtcgaca cgttgtagaa tctttcttgc gttatttatt gtcatcataa aacacctaca
    14281 gattaatgat gaggtctacg gcctcaaatc gccaatattc aatatctatc tggacaatat
    14341 tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc tggcataccc gcagaaccgc
    14401 caagatactt gctgatatcg cccggcattc tgacgggttt gaaaatcaat ttttttacta
    14461 ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa tgagtggttt tcaagttgtt
    14521 caataaaacc cggagcaact tcaggattaa tatatgtttc gtgatagaaa actttatgtc
    14581 cttccagcgc cccccagccg gtgatgcgat agagttcagt tgatggtgtc actgcaatca
    14641 gtggcgcgag cgtctcggga atatcgctgg tacggctttt ctcggtaaac ccccaggaag
    14701 gttcccgccc ttgctcaatt gcggcccgct gaaagctcgc cgacagctcc ggactgtaat
    14761 taaatcgcgg ctgggtcacg aaccacccct tacgatcctt acggaagatt ttggattctg
    14821 cctcgaggtt caacaacgcc tggcgcagcg tcatacgttt aatgcccagc agttctccca
    14881 gctctctttc tgaagggagt tttccacccg gcgtggtgat gccatctttc aaccaccgat
    14941 ccagtttctc ttttgcattt tctacggtgg attgattctc cgccattgtt taatcctttt
    15001 ggtttaaacc agatgaagca tcttacgctt acgccaaaaa agaaagcaat taacggcata
    15061 tgactttgca gataattgtg acaaagctct ctattgacgt aaccacagac ttataaggga
    15121 gatactcgca ttggtgtgcg ctcacgacag gcaaaaaaaa acctgccatc gctggcaggt
    15181 tttttataac taaaaaacga aattaatcgt ctttcggtgc gcggttggcg cgacgacgat
    15241 cgttttccgt caggtgacgt ttacgaatac ggatagaggt cggagtcact tctaccagtt
    15301 cgtcatcatc gatgaactcc agagcttgtt ccagagtcat gcggataggc ggaaccagaa
    15361 caacggcttc gtcagtaccg gaagcacgca tgttggtcag tttcttaccg gtcaggcagt
    15421 ttacagtcag gtcgttagag cggctatgaa taccgataat ctgaccttcg taaacttctg
    15481 caccgtgacc gaggaacagc ttaccgcgat cctgcagacc gaacagcgcg aacgcgaccg
    15541 ctttaccctg accgttagag atcagtacac cgttctgacg ctgacccact tcgcccggac
    15601 gtacgtcgtc gtagtggctg aaggtggagt acagcagacc cgtaccggaa gtcatggtca
    15661 tgaactcaga acggaagcca atcagaccac ggcttggaat cacgtagtcg agacgtacgc
    15721 ggcctttacc gtctggattc atgtttttca ggtcgccttt acgttcgccc agcgcctgca
    15781 ttacagaacc ctgatgctgt tcttcaacgt ccagagtcac gttttcatac ggctcttgtt
    15841 tacgaccgtc gatttcacgg aagataactt tcggacggga taccgccagt tcgaaacctt
    15901 cacgacgcat gttttcgatc agaacagaca ggtgtagttc gccacgacca gaaacgcgga
    15961 acgcatcagc gtcttcggtt tcttctacgc gcagcgcaac gttgtgtacc agttctttgt
    16021 tcagacgatc caggatctga cgagacgtta cgaacttacc ttctttaccg cagaacggcg
    16081 aggtgttaac gcagaagaac atagaaacgg tcggctcatc aacggagagt gccggcagcg
    16141 cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgccc aggcccgtga
    16201 tcgccacgat atcgccagct tccgccagat cggtttcgat acgttccaga ccgaggtggc
    16261 ccagcacttt accgactttc gcgttgcggg ttttgccttc gctatcgatg atagtgacct
    16321 gctggttcgg cttcacttta ccgcgcttga tgcggccaat gccgataacg ccaacatagc
    16381 tgttgtaatc gagctgagaa atctgcatct ggaacggacc gtcaaggtca acgtccggcg
    16441 caggaacgtg gtcaacaatc gcctgataca gcggggtcat gtcttccgcc atatcttcgt
    16501 ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac gatcgggaag tccagctgct
    16561 cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg atccacaacc caatcaggac
    16621 gcgcgccagg gcggtcaact ttgttgataa caacaatcgg cttcaggccg taagcaaacg
    16681 cttttttggt tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca accaccagca
    16741 gcactgagtc taccatggac attacacgtt caacttcacc accgaagtcg gcgtgccccg
    16801 gggtatcaac gatgttgata cggtaatcat tccatttgat agcggtgttt ttcgcgagga
    16861 tggtaatccc acgctctttc tccaaatcgt tggagtccat cacgcgctct tgggtttcgg
    16921 cacgagagtc gaacgtaccg gattgttgga gcagcttgtc taccagggtg gttttaccat
    16981 ggtctacgtg cgcgatgatg gcgatattac gcaatttttc gatcacaact ttgcctcagg
    17041 cattagaaat agcgcgttat tgtacacgga ttaatcgcac tacaaaacag gatcacaaac
    17101 atcctccgca aacaagtatt gcagagtccc tttgtgatcg ctttcacgga gcataaaaag
    17161 ggttatccaa aggtcattgc accaatatgg tgcttaatgt ttccattgaa gcactatatt
    17221 ggtgcaacat tcacatcatg gtgcagccct tttgcacgat gttaagcatg ataacgcctt
    17281 ttaggggcaa tttaaaagtt ggcacagttt tcgctttatc ttttttacgg cgacacggcc
    17341 aaaataattg cagatttcgt taccacgacg acaatgacca atccaggaga gttaaagtat
    17401 gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg ttgatttgcg
    17461 cttcaccgat accaaaggta aagaacagca cgtcactatc cctgctcatc aggtgaatgc
    17521 tgaattcttc gaagaaggca aaatgtttga cggctcctcg attggcggct ggaaaggcat
    17581 taacgaatcc gacatggtgc tgatgccaga cgcatccacc gcagtgattg acccgttctt
    17641 cgccgattct accctgatta tccgttgcga catccttgaa cctggcaccc tgcaaggcta
    17701 tgaccgtgac ccgcgctcca tcgcgaagcg cgctgaagat tacctgcgtt ccactggcat
    17761 tgccgacacc gtactgttcg gaccagaacc agaatttttc ctgttcgatg acatccgttt
    17821 cggatcatct atctccggtt cccacgttgc tatcgacgat atcgaaggcg catggaactc
    17881 ctccacccaa tatgaaggtg gtaacaaagg tcaccgtcca gcagtgaaag gcggttactt
    17941 cccggttccg ccggtagact cggctcagga tattcgttct gaaatgtgtc tggtgatgga
    18001 acagatgggt ctggtggttg aagcccatca ccacgaagta gcgactgctg gtcagaacga
    18061 agtggctacc cgcttcaata ccatgaccaa aaaagctgac gaaattcaga tctacaaata
    18121 tgttgtgcac aacgtagcgc accgcttcgg taaaaccgcg acctttatgc caaaaccgat
    18181 gttcggtgat aacggctccg gtatgcactg ccacatgtcg ctgtctaaaa acggcgttaa
    18241 cctgttcgcg ggcgacaaat acgcaggtct gtctgagcag gcgctgtact acattggcgg
    18301 cgtaatcaaa cacgctaaag cgattaatgc cctggcaaac ccgaccacca actcttataa
    18361 gcgtctggtc ccgggctacg aagcaccggt aatgctggct tactctgcac gcaaccgttc
    18421 tgcgtctatc cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg aagtacgttt
    18481 cccggatccg gcggctaacc cgtacctgtg ctttgctgcc ctgctgatgg ccggtcttga
    18541 tggtatcaag aacaagatcc atccgggcga agctatggac aaaaacctgt atgacctgcc
    18601 gccagaagaa gcgaaagaga tcccacaggt tgcaggctct ctggaagaag cactgaacga
    18661 actggatctg gaccgcgagt tcctgaaagc cggtggcgtg ttcacggatg aagcgatcga
    18721 tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc cgcacccggt
    18781 agagtttgag ctgtactaca gcgtctaata gttgaagttg tactacccgg cgcaacaacg
    18841 ccgggattta gttgccgtgg aaactttcag cccatctctg catgggcttt tttctccgtc
    18901 aattctctga tgcttcgcgc tttttatccg taaaaagcta taatgcacta aaatggtgca
    18961 acctgttcag gagactgctt tatggcaaca ggcacgcagc ccgatgctgg gcagatcctc
    19021 aactcgctga ttaacagtat tttgttaatc gatgacaacc tggcgatcca ttacgccaac
    19081 cccgctgcgc aacaactgct cgcccaaagc tcccgcaaat tgtttggtac gccgttaccg
    19141 gaactgttga gctacttctc attaaatatc gagctgatgc aagaaagcct ggaggcgggg
    19201 caaggtttta ccgataacga agtgacgctg gtgatcgacg ggcgctcgca tatcctttct
    19261 gtgacggccc agcgtatgcc ggacggcatg atcctgctgg agatggcgcc gatggataac
    19321 cagcgccgct taagtcagga acagctacag cacgcccagc aggttgctgc ccgtgattta
    19381 gtgcgcggcc tggcacatga gattaaaaat ccgcttggcg gtttacgtgg cgcggcgcag
    19441 cttctcagca aagcattacc tgacccgtca ctgctggaat ataccaaagt gattatcgaa
    19501 caggcggacc ggctgcgaaa tctggtcgac cgtctgttgg ggccgcaact gcccggtacg
    19561 cgcgttaccg aaagtattca caaagtggct gaacgcgtgg tgacgctggt gtcgatggaa
    19621 ctgccggaca acgtgcggtt gattcgtgat tacgacccca gcctgccaga actggcgcac
    19681 gacccggatc aaattgaaca ggtcttgctg aatattgtgc gcaatgcgct acaggcgctg
    19741 gggccggagg gtggagaaat cattctgcgt acccgcaccg cgtttcaact gaccttacac
    19801 ggcgagcgct accggctggc ggcgcggatt gatgtggaag ataacgggcc aggcattccg
    19861 cctcatttgc aggatacgct gttttacccg atggtcagcg gccgcgaagg tggcaccggg
    19921 cttggcttat ccatcgctcg taatttgatt gatcagcatt caggcaaaat tgaatttacc
    19981 agttggccag ggcataccga gttctcggtt tacctgccta tcaggaaata aaggtgacgt
    20041 ttatgcaacg agggatagtc tgggtagtcg atgacgatag ttccatccgt tgggtgcttg
    20101 aacgtgcgct cgctggagcg ggtttaacct gtacgacatt tgagaacggc gcggaggtac
    20161 tggaggcgct ggcgagcaaa acgccggatg tgctgctttc agatatccgt atgccgggaa
    20221 tggacgggct ggcactgctc aagcagatta aacagcgcca tccgatgctt ccggtcatca
    20281 ttatgaccgc acattccgat ctggatgctg ccgtcagcgc ctatcaacaa ggggcgtttg
    20341 attatctgcc caaaccgttt gatatcgacg aagccgtggc gctggttgag cgcgccatca
    20401 gtcattacca ggaacagcag cagccgcgta atgttcagct taacggccca acgaccgata
    20461 tcatcggcga agcgccagcc atgcaggacg tgttccgtat tatcggtcgg ctttcgcgtt
    20521 cttctattag cgtgctgatt aacggcgaat ccggcaccgg taaagaactg gtcgctcatg
    20581 ccctgcatcg ccacagtccg cgagccaaag cgccgtttat cgcgctgaat atggcagcta
    20641 tcccaaaaga tttgatcgaa tcagaactgt ttggtcacga gaaaggcgcg tttactggcg
    20701 cgaataccat tcgtcagggg cgttttgaac aggctgatgg cggtacatta ttcctcgatg
    20761 aaattggcga tatgccgctg gatgtgcaga cgcgtttgct gcgcgtgctg gcagacggtc
    20821 agttttaccg cgttggcggc tatgcgccgg tgaaagtgga tgtgcggatt atcgctgcca
    20881 ctcaccagaa tctcgaacag cgggtgcagg aaggtaagtt ccgtgaggat ctgttccacc
    20941 gcctgaacgt tatccgcgtt catctgccgc cgctgcgtga gcgtcgggaa gatattcccc
    21001 gcctggcgcg ccatttttta caggttgccg cgcgagaact gggcgtagaa gcgaagttgc
    21061 tgcatccgga aaccgaagcc gcgttgacgc gtctggcgtg gccaggcaac gtgcgccagc
    21121 tggaaaacac ctgccgctgg ctaacggtaa tggctgccgg acaggaagtg ttgattcagg
    21181 atttgcctgg cgaactgttt gaatcaacgg ttgcggagag tacttcgcaa atgcaaccgg
    21241 acagttgggc gacgcttctt gcgcagtggg cagacagagc gctacgttcc ggtcatcaaa
    21301 atctgctttc cgaagcgcag ccagagctgg agcggacgtt actgacgacc gcgttgcgac
    21361 atacgcaggg gcataaacag gaagcggcgc ggctactcgg ctggggccgc aacactctga
    21421 cgcgtaagtt aaaagagctg gggatggagt gattcacggc ttgtgtgtaa agattgatta
    21481 ttgagcgcaa attgctggta ttttgcgctt tactgttccg ataagttcag tatgatcttg
    21541 cccggaaaac ggggagagtc attatgctgg aatcaataat taatctggta tcgagtggcg
    21601 cagttgacag ccacacaccg caaactgccg ttgctgccgt gctgtgtgcc gcgatgattg
    21661 ggctgtttag ttgagtttca gattgctgat gaagtgctct ttgttcatgc cggatgcggc
    21721 gtgaacgcct tatccggtct acaaaagcaa tattttcaac aggttttgta ggcctgataa
    21781 gcgtagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
    21841 gtaaacgaag cgccattcac tatttaaatc acccgagaga actgctgcat ccgcgctttc
    21901 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
    21961 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
    22021 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaataggg
    22081 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    22141 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatccac ttgctgatag
    22201 tactgtttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    22261 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa attacgatgc
    22321 agcacgcctt cacgctgggc caccgccagc tcgtcatccg gacgggcaaa gtgatccata
    22381 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    22441 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    22501 ggcagatgcg catagttaaa gacgctcaga cggtcagggt ttagctccgc cacgcgtttc
    22561 agggtaaagg cgaaactttc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    22621 gtggaggtaa agccaatctc acgcgcatgg ttaagcagcg caaagataaa ctcttcatcc
    22681 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catactcagg
    22741 cgattaaagc cctcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    22801 acttcgatcg aaatctccgc atcggcattg aactggaagt tttcgcgcag cagcttcatc
    22861 aggcggctga tttgcgcttt attaagatac gtcggcgttc cgccgcccca gtgcagctgg
    22921 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    22981 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    23041 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    23101 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    23161 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    23221 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    23281 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    23341 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    23401 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    23461 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    23521 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggca ctcagggttt
    23581 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca
    23641 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    23701 tttcagtcac acccaatgga ataggggttt tactgccaat acgtggatct ttcggtgcgt
    23761 tctggccttt gctgcctgac gtgttgtttc cgcccgctgc gcggctgccc ggcgcatgac
    23821 cacgacgttt tttctgacgc ttgcggtcac gagcttcctg attcagctct tcgcgcgttt
    23881 tgcgacgcgc ttttgcatga cctttgctgc gtgatggtga agatggtttc ataatggttc
    23941 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    24001 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    24061 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    24121 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    24181 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    24241 tatgggtgtt actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    24301 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    24361 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    24421 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    24481 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    24541 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    24601 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    24661 aaaagcctgg ctcgtacctc aaaaacccca ggacgcaccc agcttatcaa cctgtttgaa
    24721 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    24781 gagatgaagc gcaaatggca gcgcgcgctc ggcgagtacc tcgaaaaacg tcagagccta
    24841 caaggtctgg tagtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    24901 attgagtggg cggtagacag caatatcgcc gtgctggtac tgctgaccaa agcggacaaa
    24961 ctggcaagcg gcgcacgtaa agcgcaattg aatatggtgc gtgaagcggt gctggcgttt
    25021 aacggtgatg tgcaggttga aacgttttct tcgctgaaga aacaaggcgt ggacaagctg
    25081 cggcagaaac tggatacctg gtttaacgag atgcagcctg tagaagaaac gcaggacggc
    25141 gaataatttt cttaccttaa tgcttatgcc ggatgtggcg tgtccggccc ataaattcaa
    25201 tagattacaa ttctaccgtt ggcctgataa acatggtgca tcaggcattg cggatttttt
    25261 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    25321 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    25381 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    25441 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    25501 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    25561 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    25621 cttcgcgacg gcatcgacat catctctatg aacttcaaat accagttcat cgtgtacctg
    25681 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    25741 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    25801 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    25861 cagcgtttca acgtagccct gctctttcgc ctgagcacgg gtgcgttcca tatacttcag
    25921 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    25981 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    26041 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    26101 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttgtcacg
    26161 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    26221 atcctctggc gcaataaacg cctggcggat acggcgacct tcttcgttac gtaccggaat
    26281 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    26341 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaggt
    26401 cgatttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    26461 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    26521 cggtttaatg ccctgttttt caaacagaat ggtttgtaac tgcttggtgg aagaaaggtt
    26581 aaattcctca cctgcaattt catacgcttt cttttccagc tcagccagac gaagggtgag
    26641 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccattac gttcaatgcg
    26701 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    26761 tttttgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    26821 ggcgtaacgt ccggcctcat cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    26881 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    26941 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    27001 aatcccacgc agctcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    27061 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    27121 gcgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    27181 gcctgactcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    27241 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    27301 tgtttcttca tcaaggatgg tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    27361 tggtgcttcg tctgcaacac tggtttcctg tggcttcgcg gcgggttttg cccctttggc
    27421 ctgtaaccat ttgcccgctt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    27481 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    27541 cagttcaacg tcggttttaa tcgtcgccag ctggtatgag agataagcaa cttctttgtt
    27601 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    27661 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    27721 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    27781 atcgatgatc agttctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    27841 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    27901 atcgccagtg ctgatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    27961 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    28021 catcgcgtgc aagggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    28081 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    28141 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    28201 cgcaccggtc ggttcgcctg cgctgttagt cagcggagga aacgcgtgat atgcgcgata
    28261 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    28321 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggataaaa acatgagttt
    28381 ttcaccgctt ttcgtgaaaa tcttttgtag atcttctgga tcgctcgcaa atcgtcatgt
    28441 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    28501 tatgatgttt gtctttgttt ttcatgcagt taaaaataaa gccatcccta ataattgaga
    28561 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    28621 ttatttgggt cgttttctgc taaaatctgc gccttgcgct tctttagcgc actgtctatg
    28681 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    28741 agtatcgtat tgaccatttt ggtcactatt ttttgctccg tcccgatcat cattgccggg
    28801 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    28861 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    28921 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    28981 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    29041 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    29101 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    29161 gagcgacgtg gtaaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    29221 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    29281 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    29341 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    29401 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    29461 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    29521 cgccattttc agcaatggct gaactcactg tggcaggaaa aagaccggct tctgacgtcg
    29581 ttgatgtcat cacagcggca tgacaagtaa attttgctcg cctctgaatg agcagaggcg
    29641 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    29701 cctgtccgtt aaaaacaact ttacccggtg tataatgcag ctgcagcgag gcggtattgt
    29761 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    29821 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    29881 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    29941 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    30001 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    30061 acggtaaata ctctttgaac aatgcagcat tcatttcttg cagagcaact tcatcgtttg
    30121 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    30181 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    30241 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    30301 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    30361 ccccctgatt gataatatta acctgattaa atttcgactc gctttcacca accggaaatt
    30421 tcttttcttc taatcgtgtc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    30481 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    30541 aactaccatc accattaaaa gtaaactggc cctcaccgaa agaaaacttt ccatactctg
    30601 cagaggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    30661 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    30721 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    30781 gcaacggacc gtgctcaaca tccacatcaa atgcaacttt ctggcctggc ttgatattga
    30841 gatcgggtgc accgttatca aatgaaattt gcatttgaaa atgggaatta aaaaagcctt
    30901 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttagcat
    30961 cgttaaaatc tttaataaat ttttcgacac caggctgaat ttgcgtacct gtgtaccatg
    31021 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    31081 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
//
LOCUS       DAZGBC010000043        30701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGBC010000043 DAZGBC010000000
VERSION     DAZGBC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.043"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..797
                     /gene="artJ"
                     /locus_tag="ACHK1J_001953"
     CDS             66..797
                     /gene="artJ"
                     /locus_tag="ACHK1J_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO7911226.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(848..2329)
                     /locus_tag="ACHK1J_001954"
     CDS             complement(848..2329)
                     /locus_tag="ACHK1J_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO7911227.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWDPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(2341..2661)
                     /locus_tag="ACHK1J_001955"
     CDS             complement(2341..2661)
                     /locus_tag="ACHK1J_001955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7911228.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            complement(2683..4026)
                     /locus_tag="ACHK1J_001956"
     CDS             complement(2683..4026)
                     /locus_tag="ACHK1J_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO7911229.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            complement(4306..5433)
                     /gene="rlmC"
                     /locus_tag="ACHK1J_001957"
     CDS             complement(4306..5433)
                     /gene="rlmC"
                     /locus_tag="ACHK1J_001957"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO7911230.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            complement(5474..5962)
                     /locus_tag="ACHK1J_001958"
     CDS             complement(5474..5962)
                     /locus_tag="ACHK1J_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO7911231.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6022..6867)
                     /gene="potI"
                     /locus_tag="ACHK1J_001959"
     CDS             complement(6022..6867)
                     /gene="potI"
                     /locus_tag="ACHK1J_001959"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO7911232.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPAERGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(6864..7817)
                     /gene="potH"
                     /locus_tag="ACHK1J_001960"
     CDS             complement(6864..7817)
                     /gene="potH"
                     /locus_tag="ACHK1J_001960"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO7911233.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMNHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQFSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(7827..8960)
                     /gene="potG"
                     /locus_tag="ACHK1J_001961"
     CDS             complement(7827..8960)
                     /gene="potG"
                     /locus_tag="ACHK1J_001961"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO7911234.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(9055..10167)
                     /gene="potF"
                     /locus_tag="ACHK1J_001962"
     CDS             complement(9055..10167)
                     /gene="potF"
                     /locus_tag="ACHK1J_001962"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO7911235.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(10518..10994)
                     /locus_tag="ACHK1J_001963"
     CDS             complement(10518..10994)
                     /locus_tag="ACHK1J_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO7911236.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(11082..11984)
                     /gene="rimK"
                     /locus_tag="ACHK1J_001964"
     CDS             complement(11082..11984)
                     /gene="rimK"
                     /locus_tag="ACHK1J_001964"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO7911237.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(12045..12767)
                     /gene="nfsA"
                     /locus_tag="ACHK1J_001965"
     CDS             complement(12045..12767)
                     /gene="nfsA"
                     /locus_tag="ACHK1J_001965"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO7911238.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(12751..13038)
                     /locus_tag="ACHK1J_001966"
     CDS             complement(12751..13038)
                     /locus_tag="ACHK1J_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO7911239.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            13198..13455
                     /gene="grxA"
                     /locus_tag="ACHK1J_001967"
     CDS             13198..13455
                     /gene="grxA"
                     /locus_tag="ACHK1J_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO7911240.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(13485..13862)
                     /locus_tag="ACHK1J_001968"
     CDS             complement(13485..13862)
                     /locus_tag="ACHK1J_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO7911241.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            14132..15817
                     /locus_tag="ACHK1J_001969"
     CDS             14132..15817
                     /locus_tag="ACHK1J_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO7911242.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(15992..16528)
                     /gene="rcdA"
                     /locus_tag="ACHK1J_001970"
     CDS             complement(15992..16528)
                     /gene="rcdA"
                     /locus_tag="ACHK1J_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO7911243.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVIDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            16612..17820
                     /locus_tag="ACHK1J_001971"
     CDS             16612..17820
                     /locus_tag="ACHK1J_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911244.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            17820..18635
                     /gene="ybjI"
                     /locus_tag="ACHK1J_001972"
     CDS             17820..18635
                     /gene="ybjI"
                     /locus_tag="ACHK1J_001972"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO7911245.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQDIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            18721..19005
                     /gene="ybjH"
                     /locus_tag="ACHK1J_001973"
     CDS             18721..19005
                     /gene="ybjH"
                     /locus_tag="ACHK1J_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO7911246.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(19046..20278)
                     /gene="mdfA"
                     /locus_tag="ACHK1J_001974"
     CDS             complement(19046..20278)
                     /gene="mdfA"
                     /locus_tag="ACHK1J_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO7911247.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            20563..21159
                     /gene="ybjG"
                     /locus_tag="ACHK1J_001975"
     CDS             20563..21159
                     /gene="ybjG"
                     /locus_tag="ACHK1J_001975"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7911248.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQATGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            21217..21975
                     /gene="deoR"
                     /locus_tag="ACHK1J_001976"
     CDS             21217..21975
                     /gene="deoR"
                     /locus_tag="ACHK1J_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO7911249.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(22022..23224)
                     /gene="dacC"
                     /locus_tag="ACHK1J_001977"
     CDS             complement(22022..23224)
                     /gene="dacC"
                     /locus_tag="ACHK1J_001977"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7911250.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            23471..24097
                     /gene="gstB"
                     /locus_tag="ACHK1J_001978"
     CDS             23471..24097
                     /gene="gstB"
                     /locus_tag="ACHK1J_001978"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO7911251.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDTLFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(24094..25209)
                     /gene="yliI"
                     /locus_tag="ACHK1J_001979"
     CDS             complement(24094..25209)
                     /gene="yliI"
                     /locus_tag="ACHK1J_001979"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO7911252.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(25320..25703)
                     /gene="bssR"
                     /locus_tag="ACHK1J_001980"
     CDS             complement(25320..25703)
                     /gene="bssR"
                     /locus_tag="ACHK1J_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO7911253.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            25916..27241
                     /gene="rimO"
                     /locus_tag="ACHK1J_001981"
     CDS             25916..27241
                     /gene="rimO"
                     /locus_tag="ACHK1J_001981"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO7911254.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(27288..28616)
                     /locus_tag="ACHK1J_001982"
     CDS             complement(27288..28616)
                     /locus_tag="ACHK1J_001982"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7911255.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(28624..30699)
                     /locus_tag="ACHK1J_001983"
     CDS             complement(28624..30699)
                     /locus_tag="ACHK1J_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7911256.1"
                     /translation="MPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESG
                     RVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDK
                     IHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSV
                     DTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLLTTRVF
                     RRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKL
                     LSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQ
                     RIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAIC
                     EPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIG
                     ELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIK
                     TAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEIT
                     ETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDI
                     FGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAW
                     DKSGKLVKE"
BASE COUNT         7933 a         7930 c         7250 g         7588 t
ORIGIN      
        1 ggcattgtta tcccatgccg cagacacggc caaaaatcat aagattgaca gacgggagtt
       61 ccatcatgaa aaagttagtt cttgccgcat tacttgcttc ctttactttc ggtgcttctg
      121 ccgcagagaa aatcaatttt ggcgtttcag ccacctatcc accctttgaa tctataggtg
      181 ctaataatga gattgtcggc tttgatatcg acctggcaaa agccttgtgc aaacaaatgc
      241 aggcagaatg tacttttact aatcacgctt tcgacagcct gatcccgtca ctgaaattca
      301 ggaaatatga cgccgtaatc tccggtatgg atatcacacc agatcgtagc aagcaggtat
      361 cgttcaccac gccctattac gaaaactcag ccgtcgtgat tgccaaaaaa gatacctaca
      421 aaacgtttgc cgacctgaaa ggcaaacgca ttgggatgga aaacggtact acgcaccaga
      481 aatatattca ggatcagcac ccggaagtga aaacggtctc ctatgacagt tatcaaaatg
      541 cctttatcga tctgaaaaat ggccgtattg atggcgtatt tggtgacact gcggtggtta
      601 atgaatggct gaaaaccaat ccgcaactgg gcgttgctac tgaaaaagtg accgatccgc
      661 aatactttgg caccggtctg ggtatcgctg tacgtccgga taacaaagct ctgctggaaa
      721 aactgaataa cgcgctggca gcaattaaag ctgacggtac atatcagaaa atcagcgatc
      781 agtggttccc gcaataatta ttcatttgcc ggagtgggcg aacacccact ccggcaaaat
      841 cacttctcta cgctaatccc aggcgctgaa actgctctgg cggcgcatca ttatctttca
      901 tcaactgaat cagcaacgcg atcatccggg actcgatcac atcatcatga atcggatgta
      961 gctgctgcgg atcgttttgc agatcaaaca gcaaatgacc aaactgattc aaccccttat
     1021 atttttcctg tgccgggatg cgtaatacct gtaacccttt ggtaaaacga aacggtgggg
     1081 ccagcgataa agcccgcatc tcatccaccg gatagcggct gttcattttg caggacatca
     1141 acgtgtagtt ggcaatatca tcctcacgcc ccggcagcgc tgcacgcata tacacataac
     1201 gcccatcagt tacgttagca tgaccactga aaacaccaaa catcgccgag ctgcgtactg
     1261 gttcatcgtg actgaccgtt ttcgccagcg gttgcccctg cacatcgggc ggcactggct
     1321 gttgaaaaaa gtcatacaac gttggcgccc agtcgatcat ttgtaccagt gcctgtcgcc
     1381 gctctccttt tacaccactg cgcgggtccc agataaacaa cggattattg gcaacttcgt
     1441 tgtaataagg catctggttt ttcgcccacc agccatgctc accgagtaaa aagccgtggt
     1501 cagtgccgac aatcagcatg gtatctcgcc acagatcgtg ctcatccatc aggtcgagaa
     1561 tgcgccccag gttgcgatcg cacatcgaca ccagcgaacg ataccggcaa cggatgtgct
     1621 ccacggcctc gtcgctttcc tgcacccggc cacgcggcca gtcataatgc ggtccatccc
     1681 agttatcttc atacaaggaa agatactctt ccgttgtgtg aaacggctcg tgcggatcaa
     1741 acgtttcgat ctgcaataac cagttatctt ccgcgtgatt tttgtggata aaggcacagc
     1801 ctgcatcaaa cactttagtt tgtggaaaat ccgcttcctg ctgaatatat tcccggtttg
     1861 cccagtcatg gcgccacaga ccagaaacac cgccaccggt ctgcttttgc ggcacccgta
     1921 gcacctcggg aataggcggt tgcgcaacgc ttgcatgcca gtgatcgcct tcctgtccgc
     1981 gcacaatctc ccacgaactg tagcgattat gataattccc gccgccatct tcccagtaat
     2041 gcaaatgatc gcttatcagg tgggtataga tccccgcctt tttaagcagc tccggcatgg
     2101 aatcgtcaaa cggttctaac ggcccccact cccgatgcag gaagttacat cgcccggtat
     2161 gcaactcccg ccgggcaggc atacacggca tactgccgac gtaactgttt tcaaaggtgg
     2221 cggcatgagc cgccagacgt tggaaattag gcgctttggt taacgcatcg ccataaggtg
     2281 gcagataacg tttattcaga ctatcgaaca gcagaataat cgctctcatt acgccctacc
     2341 ttattgatta attaaatgcg gaaaatgttg ctgaaccact tcataaattt gctgaccaat
     2401 agtttcaccg tccataatat tctctatcgg aatcaaatat tgcccttgtt tcgggtcaag
     2461 actaaccaca tcgagtaatt ttttggaaat agcaaaaata tccgcccagt cggtaaatcc
     2521 gccgacttca cctgcggcgg tatgttccat ttcaactttg aaatcatatt tggtcagcgc
     2581 ttttttaacg accgtttgta acataacgct actgcctaca ccgtaaccac agacaaacag
     2641 aatattaatt gtatctttca tttttaaacc cttaatattt aattaacccg cgtaaggacc
     2701 aagatggaat gttgcagcga taaatttcag aacttcacaa atagcaggcc atacggttgc
     2761 ccagtcaaat attcctgtcc agccgacgcc attttcagca agcccggtta acggaatagc
     2821 ccacaccgta ccgaaggtct gaataatacc cagcaagaac gtacagataa ttacggaacg
     2881 gtatccgccc atgcggtttg ctaatacgcc aattggaccg ccagagaaaa acagaggtac
     2941 aaagccaggc aataccataa ttgggctgtg aaccagtaat aagatcccca tcgaaaaaat
     3001 ggtgccaaaa gtacagaaga cgaagcctaa cgttgcggca ttgggtgaaa aaggtaacag
     3061 cgctgcaaca tcgacagcag gaatagcgtt aggaataaag cgatcttgcc agcctttaaa
     3121 cgaaccgtta atttcgccca ccatcatgcg cacaccctgc agcagaatga ccatatacat
     3181 ggagaactgt aagccaaggt tgatgatata gatgtaccag ggcttgcctt tcgccattaa
     3241 ctggacatta tcgatacccg ttgatagcaa gaaaccaccg acaaacagcg tcatcacaat
     3301 ggcgatagcc gttacgttat ggttaaaaat cgccagccac ccgggaagtt tcaggttttc
     3361 agcatcgtgc ttttcagggt caccaaattt atgggcgaat ttggagaaaa accacaatcc
     3421 caacatctgg ttatgagcaa tggtaaaacc ggcatctttg gtaactttgg caatgggctt
     3481 aacaatgagc gtggtggaga acgcccagaa tacacccgtt aatattccgg caataataat
     3541 ggatgtagtc cagctaaaac ccagccaaaa gacaatcaac cataataccg cctgagaatg
     3601 tgaaacaccg gtattccctg tcaaaaatat ccctttggca ccagtatacc gaccaaataa
     3661 taccagcagc aggttggtaa aaaacgccag tacaaataca taaccgacat aaccaaaatt
     3721 atcccccaac gcaatttgcg ttgccgcttt cattgcgtag gaatccatta aatatccgtg
     3781 aacaccatag gctttactta cggcttctgc taccggttta aatgttgcgg aaaactggaa
     3841 accaccaaat aacaccatca ttaatcccac catggcgctg accatgccgg taataatttt
     3901 tattggcgat ttcttatcaa gggcataacc aatacccacc agcaaaccaa ttaaaattgc
     3961 cggttcagtt aaaaacgttt gaataacatc tatgaatttg gcaaacatcg tcggaacgcc
     4021 ctccataagc gtacatttcc gttgtcaatg catgaacctt ctgctgacgt taataatgtc
     4081 tcaggaaatt ttgtgtttcg ctaagcaatg cctctgtatg tattcggtaa gtgcactgta
     4141 gtaatcaaaa cagttcaatt aaagatctta tttgattatt tgtgatttga tttgcaaaaa
     4201 tagagcaata tataaccgtg atcgatcggt tattattaat cgatcgatca cgattattat
     4261 tttggatgga tgtatgccga ggtgatattg atttgaatga tgtatttaca tttttaccag
     4321 cagcgtcagc acttcatagt gcgcggtgtg cgggaacata tcgaaaagct gcacccgttc
     4381 aatgcgataa ccaggcagtt cgcggatatc tttcgccatg gtttgggcgt tacagctgga
     4441 gtagatgata aaacgcggtg ccatcgttga gagataatca cacagcggtt taccaatgcc
     4501 gcggcgcggc gggttaacca gcaccagctc cggcacctcc ccctgagcgg tggcaaactg
     4561 agtggagtcc agcgcctgaa attgcaaacg cgttaagccc agttcagcag ctgactgctt
     4621 tgcacaggca atggcctctg gcgcaatttc gatcccggtt aactgcatgt caggcgtcgc
     4681 gcagtgtaaa ccaaagcccc ccacgccgca gaacagatcc cacatatgtt taaccggcag
     4741 ctgtcgtacc cagtcgcgcg cggtagcgta cagctggctg gcgaccgccg gattagtctg
     4801 gaagaaactt tgcggacgga tccacagcgg cacatcgtta aaacgctctg ccagcgcctg
     4861 ttgttcggtc aggtagatct ccgtctcccc ttccataatc gccatatgta ccggctgaat
     4921 attgacggtg ataactttta gctgcggcaa ttgtgcctgt aaccacggca atgccttacg
     4981 cagttgtgcc agtttggttt cagaacgcag tacaaagcgc agcatcatgc cgccatcgct
     5041 ctggctttca gtcagcagaa tgtatttcag ttcgccacgc ttacgcgcca cgttgtaggg
     5101 cgttaacccc gctcgggcga taaacggttt tagcgccgca aaaacgggcg caaatgaggc
     5161 aggataaagc gggcagtcac aaaggtcttc cggtgtgccg tcgcgatgca gcataccgag
     5221 cagtggtttt tcaacgctac cactcaccac cattttggct ttattacgaa acccttgttc
     5281 cgggcctgac accggcgcgc accattcctc aaccggaaag tcggcgagca gatttttaag
     5341 atcggcggtt ttagcggaga gttgctctgg aatcggctgc gttatccact gacaggaacg
     5401 acagcgaccc gcgtcgtaaa gtgcgcactg catacattga ccttcacatc atcaggggcg
     5461 acgattatac acattattgc aactggaaga accgccgact ggtcgaggga acgaacagca
     5521 gcatcaggat gagcatatcc ggcagcttct gcagcatcag gctatggaag atttcacgtt
     5581 ttgattcacc gggaatgctg aacagctccg gataaccgta ccccagcgaa gccgcccaca
     5641 agtaacttgc ggccgtgatt tgtgtcagca gatatagcca tcgcgcccag cgacgccctt
     5701 tcaccagtga aaacgcacac cagatctcaa tgaaaaccag caccagactg cttaaaaaga
     5761 ccagcgttaa actccaggtt tgtacgctgc gatgaatgaa ctcgccaata ccgcgcacgc
     5821 ccagcgtatt gaaaatcatc agcacgtcga ggccacggat cataataatg gcgagcgccg
     5881 ccacctgcac cagcgcaggc acattcaggc gagcatgaga tgaagatgtt ttcttaaaaa
     5941 atcccaacgt ttcgtcttcc atgaaaacaa tgccgcgaca tacgcggcat tatgtagcca
     6001 ggttggcaaa ttttagtgtc ttcagccacg tcttgcacgc tggatgtcgc gtatccgctg
     6061 tttttctgcg cgagccatca gataccaggc gataaatccg acaattccga ccgcgccgag
     6121 gatcaatgtt gccagggcgt tgatttccgg attcaccccc atccgcacgc tggaaaagac
     6181 cagcatcggt aacgtggtgg ctcccggccc ggaaacaaag ctggcgatca ccagatcatc
     6241 aagcgacaaa gtaaaagcca gtaaccagcc agaaatgatc gcgggcatga tcatcggtag
     6301 cgtaatgacg aaaaacactt tcagcggcgt cgcaccgaga tccatcgctg cttcttctat
     6361 cgagcgatcc agttcccgca gacgcgacga aataacgact gccacataag ccgtacagaa
     6421 agtgacatgc gccagccaga tggtaagcat accgcgttcc gcaggccagc caatggcatg
     6481 agcaagcgcg acgaataata acaacagcga caagcccgtg atgacatctg gcattaccag
     6541 cggcgcggtg atcataaagg caaagccatt tgatccgcga aacctgccaa agcgcaccag
     6601 caccaccgcc gcaatagttc cgaggatcgc cgccgccgtc gccgcacagg ccgcaattgt
     6661 cagacttaaa ccaaccgcac tcatcatcgc gtcatcgcgc aataattcgc cataccagcg
     6721 cgttgaccag ccggcccaca ccgtcaccag cttcgagctg ttaaacgaat agataaccag
     6781 catcagcatt ggcgcgtaga gaaaggtgaa gcccagcagc aaaatcacaa tccgccaggg
     6841 cgaacgaact accggtaaat tattcatccg tgttctccca cgcttttttg ctggtgtttg
     6901 tggaaccaca ttatcggcac aattagcagc agcaacatga tgatcgctac cgccgaggcc
     6961 accggccagt cgcggttatt gaaaaactct tgccatagca cgcgcccgat catgatgctg
     7021 tccgggccac cgagcagttc cgggatcaca aactcgccca cagccgggat aaacaccagc
     7081 atcgatccgg caataatccc acctttagtc agcggcacga tcacagtaaa gaacgttttc
     7141 agcggtcgtg caccgagatc cagcgctgct tccaccagcg aataatcaat acgaatcaac
     7201 gcggtataaa tcggcagcac cataaacggc acgtaggcgt aaacaatgcc aatataaact
     7261 gccagattgg tatgaagaat ggtcagcggt tgatcgataa cccccagcca cagcagaaaa
     7321 ttattcagca caccgttgtt ttttaatatt cccatccagg cgtagacacg aatcagaaac
     7381 gaggtccacg acggcaggat caccagtaat aataagatat tacgggtcga aggcttactg
     7441 tgcgccaccg cccacgccag cggatagccg atcagtaaac agcaaatagt cgaaatcgcc
     7501 gccacctgta acgactggag ataagcatcg aaatagagcg gatcgtcggt cagttgcaga
     7561 aaattaccga gattaagagt gatggaaaat tgcccgtcag cccactccat cagttcggta
     7621 taaggtggaa tagcgcgcgc catctctgcc aggcttattt taaagacaat cagaaatggc
     7681 agcagaaaca gcaagatcaa ccagatatac ggcaacgcaa tgaccagttt gcgcccatgg
     7741 ttcatttgca actgcgacaa ccacagctta aatccgcccg gcggtttcga ctgggcagca
     7801 ggttcaagtg tactcatctt tgctccttaa accgtaagca ccacacagct gtccacttcc
     7861 cagcacaaac gcacttcgtc gccccaggtc ggtaaccctt tacgatgacg atgggcgttt
     7921 tgtagctggg cgctaatcat ctgcccactt ttcagacgaa cgtgatacac cgaaagatcg
     7981 ccgagatagg caatgtgtat cacctccccc accgcgaagt tacaaccatt ggcgggcggc
     8041 tcttcgcaaa gcatgatttt ttccggacgc agcgctacgt gtaccggcac gttatcgacc
     8101 actgaagcat ccgcatcgac tttcagtgga tgcaccagcc ccggcgaata aagcaccaga
     8161 ccatcttcct gacgctcttt gagtacacct tcaaagacat taaccgagcc aataaattcg
     8221 gcgctatagc gggtagtcgg atgctcgtag atctcttccg gttcgccaat ctggacaaat
     8281 ttcccacgat tcataatggc gatacgcccc gccatggtca tcgcctcttc ctgatcgtga
     8341 gtaaccatca cgcaggtcac accgacgcgc tccagaatat ccaccacttc aagctgcatc
     8401 cggtcacgca gctttttatc cagcgcgccc atcggctcat cgagcagtaa tagtttcggg
     8461 cgtttggcaa ggcttcgggc cagggccaca cgctgtcgct gaccaccgga aagctgatgc
     8521 ggtttgcgtt tggcgaactc ctgcatatgc acaaggccga gcatctcatt gacccggctg
     8581 gcaatttccg ctttcggtag tttgtcctgt ttcaggccaa aagcgatgtt ctgttccacg
     8641 gtcatatgcg gaaacagcgc gtaagactga aacatcatat tgatggggcg caggtaaggc
     8701 ggcacctgtg acaaatcgac gccatcgagc ataatctgcc cggctgaagg ttgttcgaaa
     8761 cctgccagca tacgcagcag cgtggacttg ccacagccgg atgcgcccag cagcgcgaag
     8821 atttcacctt tgtagatggt caggctgaca tcatccaccg catgttgacc atcgtaggat
     8881 ttggtcaggt tgcggatttc taatagcggc gtcagcgcct tacgggtttt cgcctgcggg
     8941 cgagggatag cgtcattcac ggctgctctc cggcattgaa tgtgtgcaag cgcaccataa
     9001 tggtgcgaat tgccggcggg tgtggtgcgc ccgtccggca tctaaggctg cggattattt
     9061 tccgctcttc actttggtcc acgcgcgggt gcgcacacgg tcgattttcg gatcctgtac
     9121 tttcagagtg aacaacttcg cgcggacatc cgcaggcgga taaatacccg ggttatcacg
     9181 gacttccgca ctcaccagcg gcgtggctgc tttattggcg ttagcataga acacatggtc
     9241 agagatatgt gccaccacat caggacgcag caggtaattc aggaactgat aggcttcgtc
     9301 tttatttttg gcatccgcag gcatggcgaa tacatcaaag aacgccatcg ccccttcttt
     9361 tggaatcgag aacgagacat tcacgccatt cttcgcttcc ttcgcgcggt ttgacgcctg
     9421 ccagacatca cctgcccagc cgatagcgac gcaaatatcg ccgtttgcca ggtcgttaat
     9481 gtattgagat gaatggaaat aacgaatgtt cgggcgcagc tttaacagca gatcggtagc
     9541 cggtccggtg taatcatccg ctttagtgct gttgggatct ttgccgagat aattcagcac
     9601 ggtagcaaaa acttcttctg gcgcatcgag gaaagataca ccgcagcttt tcagtttttc
     9661 cagattttca ggcttgagga tcaagtccca gctatcgacg ggcgcgtttt cgcccagcac
     9721 cgctttgact ttatcgacgt tatagccaat cccggtcgtc gcccacatat agggcatagc
     9781 aaatttattg tcgggatcgt gtttggcgac caacttcagc agttccgggt cgagattctt
     9841 ccactccggc aatttgcttt tatccagcgg ctggaaaact cccgctgtca gctggcgttc
     9901 aagaaagctt gctgacggca ccaccaggtc aaagccggtg ctcccggcca ttaatttgcc
     9961 ttccagtact tcgttagagt cgaaaacatc gtagacgact ttaataccgg tttctttttc
    10021 aaagttagcc accgtgtcgg gggcgatata atcagaccag ttataaatgt ggagtgtttt
    10081 ttgttcagcc gcgagcgtgc cgacagagac ggccatcaga gctcccgcaa ccagacccga
    10141 aagccatttt ttatttaagg cggtcatatc tctttccttc tgaaagttcg ttaacaaacg
    10201 aacaaaagtt gtcacactga aagatatgca ataaacgtgc atatttttta tcggcgcggt
    10261 gtaagaaaag agaactctct cccggccagc actgctcgct atataaaagc atcgcaagaa
    10321 taactttagc cagggtttga gactatagct cggattaaaa aacggtattt aacaaatttt
    10381 tatgcgtatt tgctctatgt gataaagcca aatcgacaca agagaagtga aataatcttt
    10441 aagaaaaggt taaaaacagc agataatttt gcagcgcgca gccactgcac cagcgactgc
    10501 gtgctcgaaa ggagttatca gtggaggaaa tagttttcgg taacgttgtt ttgcccttca
    10561 tcgtcagtcg gtaacagcag ttgatgggca ttagcttcaa gaatgaccat cgaaatctgc
    10621 tcttcgctct gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg cataacggat
    10681 aaagactggc aaaccaccag ctttggcaga ttatcatcct gcatatcaag aaatgctttc
    10741 acggtcagcg aactggcatt gatggcagat aaatccgccg ccagcggcaa tacggctgaa
    10801 ggtcggactt ccgccatggc agaaaacagg atcgtgttat cgatcagatc gattttggca
    10861 tcaaagacac cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca gttatcacat
    10921 tcaaaaaaac tcatccccag gtcatcgagc cattgacgca gcgtatccag accaggaacg
    10981 accagcgatg tcataatttt gtacaacctc tttcgataaa aagaccggca cagcttacgc
    11041 aaaaagcgca ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc cgttttcagg
    11101 caatattctg tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc gatgtcgata
    11161 ccggtggttt tttctattcc ttccagcccc ggcgacgcat tcacttccat caccaacggc
    11221 ccgcgattag cacgcagaat atccacacca gcaacgtcca gcgccatcgt tcgcgcggct
    11281 tttatcgcga tttcacgctc ctgtggtgtg atactggcga cgcttgccgc gccgccacga
    11341 tgcaaattgg aacgaaaatc gccctctttc gcccgccgtt caatcgcagc gaccacttca
    11401 tcgccaacaa ccagacagcg gatatcgcac ccttgcgcct ctttgatata ttcctgcacc
    11461 agaatatgcg cgttcagacc gcggaaagcg tcaatcacgc tttccgccgc ctgacgtgtc
    11521 tccgccagca cgacgccaat tccctgcgtg ccttcaacca acttgaccac cagaggcgca
    11581 ccaccgacca tgtcgattaa atcgctggta tcatccggcg aatgcgcaat gcccgtgaca
    11641 ggcaggtcga tgccctgacg cgccagcagt tgcatggaac gcaatttgtc acgcgcccgg
    11701 gcaatggcga ccgactcatt gagcggatag ctccccagca tctcgaactg gcgcagtgcc
    11761 gccgtcccat aaaaggtaat ggcggtgcca atacgcggga tcactgcgtc aaaatggggt
    11821 aacttgcggc ctttgtagtg aatagaagac gccgcaggat ttatgttcat gtagcaagaa
    11881 agcggatcaa gaatttcaac caggtgaccg cgctgtatag cggcttcacg cagccgctta
    11941 cacgaataga gcgttccatc ccgggacaat atggcaattt tcaccctgca cctctctgtc
    12001 agacctggtc aaaagcccgc gtatcataca tcgacgtggc ggttttagcg cgtcgcccaa
    12061 ccctgtttgt gcaaataatc cagaataaat gggcggcttt ctttaatgat tgttcggcgg
    12121 atatgatcgc tccaggtatc ccggcgatta ttgctgccac gggtgaggta atattccgcc
    12181 agttgctcgt catactgcgc cagtgcgtct ttatccagcg gttgatagct gttttcatgc
    12241 accaaaatgg aggccggtaa acgcggcttg agatccggat tatccgcagg ccagccaagg
    12301 cacagcccaa acagcggcag aacatgctgc ggtaatttca gcagtttcgt caccgcttca
    12361 atattattgc gcaggccgcc gatatatacg ccgcccaatc ccagcgattc cgctgcggtt
    12421 aatgcattct gcgccatcat tgccgtatca acgacaccga gcaacagctg ttccgccagg
    12481 ccgagctgag catccggaca gatctgtaaa tggcggttaa agtcggcaca gaacacccag
    12541 aactccgccg cttgcgctac gtgtttttgc ccgccggtca gcgtcaccag ctcttcacgt
    12601 aatgctttgt cggtaatgcg aataatgcta ctacactgca aaaaactgga actggacgtc
    12661 gcacgggcgc tgttaataat cgcctcacgc tgcgcttcgg aaatgggttc atcagtgaaa
    12721 tggcgaatgg agcgatggcc acaaataagt tcaatggttg gcgtcattat ctttttctct
    12781 ttctgaacgt gaatattgcg gtggacggtt catcagctgt ggggcaagac gttttgccac
    12841 ctgaagaata accaccaccg cagcgggaag catgagcaaa acaccgagaa aaatcatcag
    12901 aatctgcact tctggccgag aaaatggctc aggcagcgac agggagtcgc ttaccgacag
    12961 cagcgccacc gccagcaaca tcattccgat aaattccagt atcaacacgc ttttaggcaa
    13021 tttaccgatc gcgcgcatac gcttccctct gcaaagtgag ccttcagtct aaaacttttc
    13081 actgtattgt gtttaacagt tatagctttt agcaattaat gcaacaggtt aaacctactt
    13141 tcagcgaata cattttagcg tgatcattac aggcataaat ctatgaggag agaaataatg
    13201 caaaccgtta tttttggtcg cccgggttgc ccttactgtg tgcgtgcaaa agatctggct
    13261 gagaaattga gcaatgaacg cgatgatttt cagtatcagt atgtagatat tcgtgcggaa
    13321 gggatcacta aagaagatct acaacaaaag gcaggtaaac ccgtagaaac cgtgccgcag
    13381 atttttgtcg atcagcaaca tatcggcggc tataccgatt ttgctgcatg ggtgaaagag
    13441 aatctggacg cctgatcgtc tgacaagccc tcgcgttgag ggctttactg attttttctg
    13501 tgctgtggtt taaacaagct actgataaat aagaaacaca gtgcccccag cgcacaccag
    13561 aacaccgcgc ttagtaacca tgccagctct tgccagaatg agcgggtcgg tgaaaaaaac
    13621 agccgcataa taagcattga acagggtgcc gccagcattg cgccaaatag aggtttcagg
    13681 acttctctac gctgtgaaaa gaaactggca actgctccag gaagaatgaa aaacagcaag
    13741 ccgatttcag gatgcccggc agcccgaaaa gcgcctttca tgtgcgtcgc cagaaaaagg
    13801 cacaccacaa tgaagaggac aaaacagcag attgcccccg cccaacgttg tttatgtttc
    13861 actcgttcct cctgacactg cgtctatcga acacattttt cgccagtgtg gcgttcagta
    13921 agataaagcc gcttcgcatt ccatgctaat ataggccaac gcaattcata tagccgttga
    13981 tacctaatgt gattacacta gtaaaatata ttgttacttt actatcgttt aggtgcgctg
    14041 aatgaatctg cgccctgaat tctggtaaaa aacattatcg taaattacca tttctttcaa
    14101 cagcttacta gtaaacaaga agttagcctc cgtgaatata aacgtcgccg aattgttaaa
    14161 tgggaattac attctgttat tatttgtggt cctcgcgctt gggctatgtc tcggaaagtt
    14221 acgacttggt tcgatccaac tgggtaattc cattggcgtt ttagtcgtat cgctgttatt
    14281 aggccaacaa catttcagca ttaacaccga tgcgcttaat cttggcttta tgctgtttat
    14341 tttctgcgtc ggggtcgaag ccggaccgaa ctttttttcc attttttttc gcgatgggaa
    14401 aaattaccta atgttagcac tggtgatggt tggcagtgcg ctggtgatcg ccttagggtt
    14461 aggtaagctg tttggctggg atattggcct gacggccggt atgttagcag gctctatgac
    14521 gtcgacaccg gttctggtcg gtgctggcga tacactgcgt cattccggca tggaaagcag
    14581 gcagctctca ctggcactgg ataatctgag cctcgggtat gccttaacct atttaatcgg
    14641 tctggtgagt ttgattgttg gtgcgcgtta cttgccgaaa ttgcagcatc aggacttaca
    14701 gaccagcgcc cagcaaatcg cccgcgaacg tggcctggac actgatgcca accgtaaggt
    14761 ttatttaccg gtgatccgcg cctatcgcgt cggcccggag ctggtggcct ggaccgacgg
    14821 caaaaatctg cgtgaactgg gtatttatcg acaaaccggc tgctacattg aacgtattcg
    14881 acgtaacggg attctggcaa atccagacgg tgatgccgtg ctacaaatgg gcgatgaaat
    14941 agcgttggta ggctatcccg acgcccatgc ccgactcgat cccagcttcc gtaacggcaa
    15001 agaagttttc gatcgtgacc ttctcgacat gcgtatcgtc actgaagaag tggtcgttaa
    15061 aaaccataac gccgttggca aacgtctcgc acaactgaag ttgaccgatc acggttgctt
    15121 ccttaaccgc gttattcgta gccagattga gatgccgata gatgacaacg tcgtgcttaa
    15181 caaaggtgac gttttacaag tcagcggcga tgcccgccgc gtaaaaacca tcgccgatcg
    15241 catcggcttt atctcgattc acagccaggt cactgacttg ctggcattct gcgccttctt
    15301 tgttattggg ctgatgatcg ggatgatcac attccagttc agcacattca gtttcggcat
    15361 ggggaacgct gccgggttgt tattcgccgg aattatgctg ggctttatgc gtgctaacca
    15421 cccgaccttc ggttacattc cgcagggtgc attaagcatg gtgaaagagt tcggcttaat
    15481 ggtgtttatg gcaggcgttg gtctgagcgc cggtagcggt attaataacg gtctgggcgc
    15541 gattggcggt cagatgttga ttgccggatt gattgtcagt cttgtgcccg tggttatctg
    15601 tttcttgttc ggtgcttatg tattgcgaat gaaccgcgca ctgttgttcg gcgcaatgat
    15661 gggcgcacgc acctgcgcgc cggcaatgga gatcatcagt gatacagctc gcagtaacat
    15721 cccggcgctg ggctatgcgg gcacctacgc aatcgccaac gtcctgctga cgctggcagg
    15781 gacaatcatc gtcatggtat ggccaggatt aggataaaac tgaagttgcc ctgaaaatga
    15841 aatttttttg cacaaccgca gaacttttcc gcagggcatc agtcttaatt agtgccactg
    15901 cttttctttg atgtccccat tttgtggagc ccatcaaccc cgccatttcg gttcaaggtt
    15961 ggtgggtttt ttgttgccta aaatttatct actacccagc aaccctctca accatcctca
    16021 aaatctcctc gcgcgatagc ggcttacggt cggtgacaaa atgcagcgtc atcccctcaa
    16081 taaacgcatc aagcgcgcgg gcggttccgg gttcaaacca ttgttcgagc gtttgctgac
    16141 tgcgctgcat ccagttttgc attaccgttt ttaatagcgg ttttcggcta gccagcgcgt
    16201 agagctggta catcagctcc atgttatccg gcgttgccac ctgtgagctg tagatcatat
    16261 cggtgatagc ctggcatgcg ccttgagcat caataacatc gctaaaaaat gcctgatatt
    16321 gccgggacat gatctcagta aaactgctga acgcctccag caacaactca tcaattcctg
    16381 aaaaatagta ggtcatcgat cccaacggta ccccggcaag ggtagcaatt ttgcggtgcg
    16441 taacagcatg tattccgtaa agtttcaccg cctccagcgt ggcctggata attttttctc
    16501 gccgttgcgg atcgttagcg cgacgcatgt catttcctct atctgcaatt gtgtacaaat
    16561 gtacacaacc ttgctaatgt tgtcttccct cttcttattt tgacgtgatc tatgaccgta
    16621 aattcttcac gtaatgcatt gaaacgccga acctgggcgc tgtttatgtt cttctttttg
    16681 ccgggcctgt taatggcgtc ctgggcaacc cgtacgcctg ctatccgcga tattctctct
    16741 gtctcgatcg ctgaaatggg cggtgttctc tttggtctgt cgatcggttc gatgagcggt
    16801 attctctgct cggcgtggtt agtgaaacgc tttgggacac gtaatgtcat cctggtcacc
    16861 atgtcctgcg cattggtcgg gatgatgata ttaagtctgg cactctggct gacatcgccc
    16921 ctgctctttg ccgttggtct cggcgtcttt ggggcaagtt ttggttctgc ggaagtggcg
    16981 ataaacgttg aaggtgccgc cgttgagcga gaaatgaata aaacggtttt gccgatgatg
    17041 cacggttttt atagcctggg cacgctggca ggcgctggtg tcgggatggc actgacggcc
    17101 tttggcgttc cggcaacggt gcacatttta ttggcggcgc tggtaggcat cgcgcctata
    17161 tatatcgcca ttcaggcaat ccctgacggt acgggcaaaa atgctgccga tggcacccag
    17221 catggcgaaa aaggcgtacc tttttatcgc gatatccagt tgctgctgat tggtgttgtg
    17281 gtgctggcga tggcctttgc cgaaggttct gccaacgact ggttaccctt attaatggtt
    17341 gacggtcacg gttttagccc cacttccggc tcgctgattt atgccggttt taccctgggg
    17401 atgaccgttg gacgcttcac tggcggttgg ttcatcgacc gttacagtcg cgttgccgtg
    17461 gttcgggcca gtgcgctaat gggggcgttg ggtattgggc tgattatttt tgtcgatagc
    17521 gcctgggtcg ctggggtgtc tgttgtactc tggggactgg gtgcctcgct gggcttcccg
    17581 ctgaccattt ctgccgccag cgataccggc cccgatgcac caacccgcgt cagtgtggta
    17641 gcaacgaccg gttatctggc tttcctcgtc gggccgccgc tgctgggcta tctcggcgaa
    17701 cattatggat tacgtagtgc aatgctggtt gtactggcgc tggttattct cgcggctatt
    17761 gtcgcgaaag ccgtcgccaa acccgatacc aaaacgcaga cggcgatgga gaatagttga
    17821 tgagcattaa attaattgcg gtagacatgg atggtacttt cttaagcgat caaaaaacct
    17881 ataaccgtga gcggtttatg gctcagtatc agcaaatgaa agcacaagac attcgctttg
    17941 tggtcgccag cgggaatcaa tattatcagt tgatctcttt cttccctgaa attgctaatg
    18001 aaattgcctt tgtggctgaa aacggcggct gggtagtgag tgaaggcaaa gatgttttta
    18061 atggcgagct gtcgaaggat gcgtttacta ctgtcgtgga acatttgctg acgcgcccgg
    18121 aagtggaaat tattgcctgt ggaaaaaata gcgcctatac gctcaaaaaa tatgacgatg
    18181 ccatgaaaac ggtggcggaa atgtattatc accgtctgga atacgtcgat aactttgaca
    18241 acttagaaga tatcttcttt aagtttggcc tgaatctttc cgatgaactg attccacaag
    18301 tacaaaaagc attacatgag gccatcggcg atattatggt gccggtccac accggcaacg
    18361 gcagcatcga tctgattatc cccggcgtac ataaagccaa tggccttcgc caactgcaga
    18421 aattatgggg aatagacgac agcgaagtgg tggtctttgg cgatggcggt aacgatattg
    18481 agatgctgcg tcaggcaggc tttagttttg caatggaaaa tgccggcaac gcggtcgtcg
    18541 cagcggcaaa ataccgggca ggctccaata accgtgaagg cgtactggat gtgatcgata
    18601 aagttcttaa acacgaagcg ccatttgacc aataaaaacg gcagcacgcc cggcaacaca
    18661 atacctgaat gaaggctgca taattcgcgg aaattaaccc gctgcattat ttcaggcgaa
    18721 atgtttatga aaaattgtct actgttgggc gcgcttttaa tgggctttac tggtgtggcg
    18781 atggcgcaaa gtgtcaccgt cgatgtgccg agtggataca aagtggttgt ggttcctgat
    18841 tcagtcagcg ttccacaagc ggtcagcgtc gcgactgtgc cgcaaaccgt ctatgtggct
    18901 cctgcgccag ctccggctta ccgcccgcat ccttatgttc gccatcttgc cagcgtaggt
    18961 gaagggatgg ttatcgaaca ccagattgac gaccatcacc attaattcga gaatgcctga
    19021 tcgcacaaag caatcaggca tttttttacc cttcgtgaga atttcccatc tgtttatctt
    19081 ttaaaaagat aaccatcagc gacagccaca aaattccgtt gacaaggttg aagagattaa
    19141 acagtccgtt gcccccgttc agccaggcat gtttgctgat ttcaataccg acggtaaaga
    19201 tcagcatttg cagcattccc atcgccgcag aaaccgtacc tttactcata tcgctggcaa
    19261 acagggttaa tcgcaccagt cccgcattcg ccagaccaat accgaaagca taaatactta
    19321 acccggcggt catccataaa tacgcgtgcg atgagataac cgttgccgca gcagcgacca
    19381 acagaccaat cataatcggc cagccgccca taataatcag cgaacgtacg gtgcggcgcg
    19441 aggtcagacg cgctaacagc aagttacccg caattaacgc cccgaaaata ggcacttgca
    19501 gcaaaccata ttcatagctg ctcaactgct cgccggtaat gatgataatc ggcgactggg
    19561 cgatccacgc gagcaacggc aggctaacga atcccagcgc cagcgccccc gccacaaagc
    19621 gaccgttctt cagcaccagc ttatagtcac gaccgagttc tttcagcgac agtttctcgc
    19681 ctatacgcgt ggcggtttca ggcatggctc gttgcagacc gaaaaaggag atcgctgcca
    19741 atgcggcaaa caagacaaac atcccttccc agggcagcac atggatccac gccgcgccca
    19801 ccagtggacc aagtagcgga gcaatcagcg ccacgttcgc catcagcgcg gtgatcttga
    19861 tacaaaccgc ctcttcgaag gattcctgaa ttgcggcgta tcccacagcg ccaatgaaac
    19921 agaggcttat gccctgcaag aagcgcaaca gggtgaattg ttcaatgttt tgcgccagta
    19981 atattgccag acaggtgaca ataaaccaca ccacacccgc cagcatcacc ggacggcgac
    20041 caatacgatc cgacaacggc cccaggagcc attgtaaaaa catcccgccc gccagatatg
    20101 cggtcatcga agtaggaacc caatcaatgc ccgcctgata ttgttccacc acggccaaca
    20161 taccgggttg aatcatatcg ttgccgatat aggttgaaaa ttcgtacagc accagacaga
    20221 gagggaaaag taacgcctga cgtccaagcc tggcaccgga agctaattta ttttgcatgc
    20281 aatttcttcg ccaataataa tcgcgcaaag tttaataaaa gcgcagctaa cgagaaagcg
    20341 aattttgtag ctgaaaccgc ggttaagcac attcttacat tattgcgagt atagctacgc
    20401 tttctttaag ttttatttaa cctatgcccg ttacaatcac ccaccgtaaa caggccgctt
    20461 gagggaaata agacgatgcc gctttaccca gtttaacctg cactttattc tcaacgagtt
    20521 gcctgtattg gctccctttt aatcactttg cgtcgggaag ttatgctgga aaatttgaat
    20581 ctctctctat tctctcttat taacgcgacg ccagactcgg ctccgtggat gatctcgttg
    20641 gcgattttta ttgctaaaga tttgattacc gtggtgccgc tgctggccgc ggtactttgg
    20701 ttgtgggggc ttacagcaca acggcaactg gtgataaaaa tcgctatcgc gctggcggtc
    20761 agcctgtttg tgtcctggac gatgggacat ctttttccgc acgaccgacc ctttgtcgaa
    20821 aatatcggct ataacttcct gcatcatgcg gcggatgact cattcccgag cgatcacggt
    20881 acggtgattt tcacctttgc actggcattt ttatgctggc atcgcctgtg gtccggctca
    20941 cttttaatgg tgctggccgt cgtcattgcc tggtcgcgcg tttatcttgg cgtccactgg
    21001 ccgctggata tgctcggtgg attgctggca ggtatgattg gctgccttag tgcccagatt
    21061 atctggcaag cgacagggca taaactctat caacgtctgc aatcgtggta tcgcttctgt
    21121 tttgcattac cgatccgcaa aggctgggtg cgtgactgat ctctgctaaa aagtgtagta
    21181 ttgagcggct cgcttcaata actattcaga gggattatgg aaacacgtcg cgaagagcgt
    21241 atcgggcagc tgctgcaaga attaaaacgc agcgataagt tacatcttaa agacgccgcc
    21301 gcgctgcttg gggtttcgga gatgacgatt cgtcgcgatc tgaacaacca cagtgcgccc
    21361 gtcgttttgc tcggcggcta tattgttctg gaaccgcgca gtgccagcca ttacctgtta
    21421 agcgatcaaa aatcccgcct ggtggaagaa aaacgccggg cggcaaaact ggctgcgacg
    21481 ctggtagaac ccgatcagac cctctttttt gactgtggca ccaccacgcc gtggattatt
    21541 gaagcgattg ataatgaaat acctttcacc gccgtttgtt attcgctaaa tacctttctg
    21601 gcgctgaaag aaaaacccca ttgccgcgca tttctgtgcg gtggtgaatt tcacgccagc
    21661 aacgccattt tcaaacccat cgattttcag caaacgttga ataatttttg cccggatatc
    21721 gctttttatt ccgcggcggg cgtgcatgtc agtaaaggcg ctacctgttt taatcttgaa
    21781 gagttgccgg taaaacactg ggccatgtcg atggcgcaaa agcatgtgct ggttgtcgac
    21841 cacagtaaat ttggcaaggt gcgtccggcg cgcatgggtg acctgaaacg ctttgatatt
    21901 gtggtgagtg attgttgtcc ggaagatgag tatgtgaagt acgcgcagac gcaccgtatt
    21961 aagttgatgt attaatgacg tataaccgga tgacgtttcg caccatccgg ttatcagaag
    22021 attaagagaa ccagctgccg aaccactgat ggaatttcat catcacgaaa tcccacatcc
    22081 gaccaaagaa tccgccctct tccacatttt ccatcacgat cagcggacgc tgctcaatgg
    22141 atttaccgtt aagctggaaa tcaatggtgc cgacaacctg accttttttc agcggtgcgg
    22201 taagctgcgg ttccgttaac gtgtagctcg ccttcaggtt tttcagctgc ccacgcggta
    22261 tggtcactga gcccgcttcg cctgccccga gattcacttc gctcttatca ccaaaccaga
    22321 cgcgctgagt cacaaaggtg gcatcaggtt taattggcgt cacggtttca aagaagcgga
    22381 aaccccaggt caataatttc tcagactcat taaaacggat acggtcagtt ttcgccccca
    22441 gcactacgga gattaaacgc atatcgccct gggtagccga agcaaccaga ttatatcccg
    22501 cgcctgccgt ggttcctgtc ttcatgccat caacattcag attgctgctc cacagcagac
    22561 ggttacggtt aggctgacga attttgttga aggtgaactc tttctcttta tgaatggcgt
    22621 actcttccgg cacatcgtgg atcaatgctt tacccagcaa tgccatatcg cgcgcggtac
    22681 tgaactgccc cggcgcatcc agaccgtgca ccgtctggaa ggtagtgttg gtcagaccca
    22741 gttttttggc ataaccattc atcaaaccaa taaatgactc ctggctcccg gcaacgtaat
    22801 cggccagcgc aatacaggcg tcattaccgg actggataat cacacctttg ttcaagtctg
    22861 ccaccgaaac ctgatcgccc ggtttgagga acattaccga tgaaccacgc agtgccggat
    22921 ttcccgtcgc ccaggcatct ttaccgaccg tcaccatatc ggtgagttta atcttatcgg
    22981 ccttaagcgc ctgcccaacc acatagctgg tcatgatttt agtcaggctc gcgggatcca
    23041 gtttctcatc cgcattgcct tctgccagca ctttaccgct ggcgtaatcc attaaaatcc
    23101 atgcacgcgc atccacgctc ggcgcttcaa cggtttgttc ggccgcgaat gccgttgggg
    23161 caaaaaggaa taaaaatgca gaacccgctg caagaccacg aaggagagag gagtattgcg
    23221 tcataaatgc cacccgagta tccattcaaa aaaaatacgt tatatcgccg tatgataagt
    23281 tcggtgatta ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa
    23341 gtttatgcaa tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct
    23401 taaaagttaa ctttacttca ccatcggagt cctgttcagc ctgtggctgc ctgtcggaaa
    23461 aggggtgagt atgattacgc tgtggggtcg gaataattca accaacgtaa aaaaagtatt
    23521 gctgacgctc gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg
    23581 gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg
    23641 tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc
    23701 acagtacggg caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg aagcagaaaa
    23761 atggatggac tgggcaaacc agacactcag taatgctcat cgcgggatcc tgatgggatt
    23821 agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg
    23881 cgacaccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctctggcga
    23941 cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaatgttgg
    24001 cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc
    24061 cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa
    24121 ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc
    24181 acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt
    24241 gacgctcatc acaatgacat ctttatcttt cagcgcgcca ataaataatt tttgctgcca
    24301 ctgggggaat ttatcgctgt tatagaaggc catgccgctc acggcgggcg aatctttcca
    24361 gtaaaaaaca ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa
    24421 gcctgaatag ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg
    24481 gatattaatt tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca
    24541 cggattcatc gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc
    24601 accggattcc tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac
    24661 cagtttgccc tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag
    24721 agcaataaaa agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt
    24781 agacagtttt ggcatctggc gaaagacggt gcgaaagtcg gtcacttttg agagatcatc
    24841 acttaagcgg ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata
    24901 acttaaccag atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc
    24961 gccctgcccg tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc
    25021 tgcttgccag tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg
    25081 taaaaaggcc agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt
    25141 tgcaggagcc gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg
    25201 tcgatgcata acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac
    25261 aaattcattt ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt
    25321 caggccttct caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc
    25381 agacaatttt caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca
    25441 ctcatcaaac atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac
    25501 tcttcctgat ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt
    25561 ttaaaaaagt tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc
    25621 ctcagttgca cgtatgcggc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg
    25681 attcgctgtc tgtcaacgaa catacttcga tcctcctctt tccagccttg ttacaattaa
    25741 atagtatggc tattttttga gcaaaatgct gtccaggatc aattcttcac tccgaattaa
    25801 caaaatttac atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca
    25861 gaactctgta gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag
    25921 caaagtaact ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt
    25981 tgattcagag cgtattctca ccgaactacg cactgaaggt tatgacgtgg taccgagcta
    26041 tgacgatgcg gacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga
    26101 atcactggaa gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg
    26161 tctgggggca aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg
    26221 gcctcatagc tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca
    26281 caacccattc ctgagcctgg tgccagaaca aggtgtcaaa ctgacgccgc gtcattatgc
    26341 ctatctgaaa atttctgaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat
    26401 gcgcggcgac ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt
    26461 agatgcgggc gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga
    26521 tgttaaacat cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct
    26581 gtgcgaacag ttatcgaaac tggggatctg gacacgtctg cactacgttt atccttatcc
    26641 gcatgtggac gacgtcatcc cactgatggc ggaaggcaaa atcctgccgt atctggacat
    26701 tccgttgcag cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga
    26761 tcgccaactg gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc
    26821 aacctttatt gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt
    26881 cctgaaagaa gcacgtctgg accgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc
    26941 agacgccaat gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg
    27001 tttcatgcag ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga
    27061 aattctggtg attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga
    27121 tgcaccggaa atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga
    27181 tatcctgcgt gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta
    27241 attaattcag gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta
    27301 atctgtgccc tgccgttttt cttactctta tacagccgct catccgccag cattttcaag
    27361 cgttcgacgg tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg
    27421 cttcccacct ggaaatgaac gatttctttt agaatcgata gcaactcttc cagcgttcca
    27481 cccgtataaa tcaccgcaaa ttcgtcaccg ccgaagcggt agactttacc tttatctacc
    27541 gggatctgtg atttaaggat tttcaccact ttcatcaaca cttcatcacc cagcaaatga
    27601 ccgaaggtgt cgttaatgtt tttgaattta tcgacgtcaa acaggcacaa ataaccgcct
    27661 ttttccggca gcgcttttaa atcttcgtca aaggattttc tgcccattgc tttggtcagg
    27721 tcgtccagga acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag
    27781 aaaagcccgc catccagtac gcgttcaaaa tggttattaa taatggcttc tttctccaca
    27841 ataccacgat taatcactct gcgtaccaga tagaggcaac tggtacccga tatcactgcc
    27901 accaatgcgg caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac
    27961 gcaaatacgt acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg
    28021 aagatggcgt ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaacaga
    28081 gagaacatca ggttctcgct atccatcgcc agagattttg acagtaaaga aaaacgctcc
    28141 agaccaagca aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag
    28201 gttttaccgt caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc
    28261 ataatatctg cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa
    28321 atcagataag gaccgacatc gtacaaataa gagggtgaaa ccgagtcggc gtaagctgtg
    28381 cgtaagccaa ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc
    28441 acgctacgcc gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt
    28501 ttcaggctat acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa
    28561 atcatgataa aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt
    28621 aaactactct tttactaatt ttccactttt atcccaggcg gagaatggca tgggtttctg
    28681 gtacagatac ccctgagcct gaacacatcc taatttgatt aattcgcgcg caacgtcttc
    28741 gctctctacg ccttccgcaa tcaccgggat ccggttatag cgtgccaggt cgaaaataat
    28801 gcgcacaatt tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat
    28861 gctgtctgga ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc
    28921 aatcgatatc cgtacccctt gtttacgcaa ttgttcaata ttgagataaa aatgttcatt
    28981 aattcgttcg attacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt
    29041 ttctttcata atggtggtaa aacgctcatg aaagtgtggt tcgtttaact gccagggcga
    29101 aacattaata tgcagtaaga aatcatcctt cagcgacgca cgctgactaa aatgtctgaa
    29161 ttcacccatt gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc
    29221 agcaatggta atgaacttca gcggtgaaat gatcccccgc tccgcagaca cccaacggca
    29281 caacgcctcc gcgcccagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag
    29341 gaatagttcg ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc
    29401 tatttcgttg agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat
    29461 actggaaatc gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg
    29521 ttcacaaatg gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc
    29581 gtttaccatc gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata
    29641 gagatcggca ttgaatgtca cgatatcgca gcattgtgca tatttctcgg caattcgctg
    29701 acacaatttc ctgacgattt tattgcgttc atcaacacca aacaccgctt ccacggcttc
    29761 cagaccacac agtttgatga tccccacagc cagatactga ttattgttgg actcaataat
    29821 cgccatattt ttaataaacc cactttcctg atcgtaagag atcagtgaga gcaatttatt
    29881 tgaccgttca atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc
    29941 tttcgcatcg ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt caggcaaaaa
    30001 cgccagcgta ttaaccagct ctgtcatttc agaaaattgc cgacggaaca cacgagtggt
    30061 cagtaaataa accaccagca atacgataat aatgaacgca aacggcagat aaaaaagttt
    30121 atctgatgcg ttcaataaat taccgcccgg ataataaaaa tagatttcga ataatgcgtt
    30181 atttggcacc gctgatgttt tttcgatata ttcagtatcg acagaatcaa taatattata
    30241 attaccttcg ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat
    30301 ctcatggcct tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc
    30361 gttcagatat cgccagttta aatcgacatg aattttatcc accgctaaca aatcgtgatc
    30421 tttgccgttg atgcgtagcg taaaagagat acatttttta cccggacgat caaagcaatt
    30481 gtatgccgga ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga
    30541 tgggttaaaa tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg
    30601 ttcctgatga taaacaacga ttttatcgtt cgccctccgc gaaaagagat aatatttatt
    30661 atcatcaagt accagtccca ccgagtctaa aaatggcatc a
//
LOCUS       DAZGBC010000044        30631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGBC010000044 DAZGBC010000000
VERSION     DAZGBC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.044"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            219..1802
                     /gene="yegH"
                     /locus_tag="ACHK1J_001984"
     CDS             219..1802
                     /gene="yegH"
                     /locus_tag="ACHK1J_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7911257.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2254..4107)
                     /gene="asmA"
                     /locus_tag="ACHK1J_001985"
     CDS             complement(2254..4107)
                     /gene="asmA"
                     /locus_tag="ACHK1J_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO7911258.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     TAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQILRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4129..4710)
                     /gene="dcd"
                     /locus_tag="ACHK1J_001986"
     CDS             complement(4129..4710)
                     /gene="dcd"
                     /locus_tag="ACHK1J_001986"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO7911259.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4802..5443)
                     /gene="udk"
                     /locus_tag="ACHK1J_001987"
     CDS             complement(4802..5443)
                     /gene="udk"
                     /locus_tag="ACHK1J_001987"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO7911260.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5761..9078
                     /gene="dgcE"
                     /locus_tag="ACHK1J_001988"
     CDS             5761..9078
                     /gene="dgcE"
                     /locus_tag="ACHK1J_001988"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7911261.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9187..10035)
                     /gene="alkA"
                     /locus_tag="ACHK1J_001989"
     CDS             complement(9187..10035)
                     /gene="alkA"
                     /locus_tag="ACHK1J_001989"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO7911262.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMSLKRAEALIHLANAALEGSLPMAIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10169..11521
                     /gene="yegD"
                     /locus_tag="ACHK1J_001990"
     CDS             10169..11521
                     /gene="yegD"
                     /locus_tag="ACHK1J_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7911263.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQRVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALEEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11534..13474)
                     /gene="yegI"
                     /locus_tag="ACHK1J_001991"
     CDS             complement(11534..13474)
                     /gene="yegI"
                     /locus_tag="ACHK1J_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO7911264.1"
                     /translation="MKPTLYTATGECVTTGRELGKGGEGAVYDINEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPAVQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSAVSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRT
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMELLRQAAEKLAQAQADLSRC"
     gene            complement(13471..14232)
                     /gene="pphC"
                     /locus_tag="ACHK1J_001992"
     CDS             complement(13471..14232)
                     /gene="pphC"
                     /locus_tag="ACHK1J_001992"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO7911265.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMAGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14229..14888)
                     /gene="yegL"
                     /locus_tag="ACHK1J_001993"
     CDS             complement(14229..14888)
                     /gene="yegL"
                     /locus_tag="ACHK1J_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7911266.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15109..15168)
                     /gene="ibsA"
                     /locus_tag="ACHK1J_001994"
     CDS             complement(15109..15168)
                     /gene="ibsA"
                     /locus_tag="ACHK1J_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO7911267.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15441..15497)
                     /locus_tag="ACHK1J_001995"
     CDS             complement(15441..15497)
                     /locus_tag="ACHK1J_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7911268.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15769..15825)
                     /locus_tag="ACHK1J_001996"
     CDS             complement(15769..15825)
                     /locus_tag="ACHK1J_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7911269.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16104..17351
                     /gene="mdtA"
                     /locus_tag="ACHK1J_001997"
     CDS             16104..17351
                     /gene="mdtA"
                     /locus_tag="ACHK1J_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO7911270.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNEPQSAAPGATKQAQ
                     HPPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATAPEEKATSREYAKKGARS"
     gene            17351..20473
                     /gene="mdtB"
                     /locus_tag="ACHK1J_001998"
     CDS             17351..20473
                     /gene="mdtB"
                     /locus_tag="ACHK1J_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO7911271.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKIFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMLAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20474..23551
                     /gene="mdtC"
                     /locus_tag="ACHK1J_001999"
     CDS             20474..23551
                     /gene="mdtC"
                     /locus_tag="ACHK1J_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO7911272.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23552..24967
                     /gene="mdtD"
                     /locus_tag="ACHK1J_002000"
     CDS             23552..24967
                     /gene="mdtD"
                     /locus_tag="ACHK1J_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911273.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPIGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24964..26367
                     /gene="baeS"
                     /locus_tag="ACHK1J_002001"
     CDS             24964..26367
                     /gene="baeS"
                     /locus_tag="ACHK1J_002001"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO7911274.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26364..27086
                     /gene="baeR"
                     /locus_tag="ACHK1J_002002"
     CDS             26364..27086
                     /gene="baeR"
                     /locus_tag="ACHK1J_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO7911275.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27277..27609
                     /gene="yegP"
                     /locus_tag="ACHK1J_002003"
     CDS             27277..27609
                     /gene="yegP"
                     /locus_tag="ACHK1J_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO7911276.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27807..28104
                     /locus_tag="ACHK1J_002004"
                     /pseudo
     CDS             27807..28104
                     /locus_tag="ACHK1J_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            28106..28402
                     /locus_tag="ACHK1J_002005"
     CDS             28106..28402
                     /locus_tag="ACHK1J_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7911277.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28505..29866
                     /gene="yegQ"
                     /locus_tag="ACHK1J_002006"
     CDS             28505..29866
                     /gene="yegQ"
                     /locus_tag="ACHK1J_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO7911278.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30196..30513)
                     /locus_tag="ACHK1J_002007"
     CDS             complement(30196..30513)
                     /locus_tag="ACHK1J_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911279.1"
                     /translation="MKKIVAISLFSIFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CVE"
BASE COUNT         7058 a         8158 c         8080 g         7335 t
ORIGIN      
        1 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
       61 aatgttttat tattaccact tttaattcag ggataatgtg aggttattac cctcaaataa
      121 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
      181 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
      241 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
      301 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
      361 ttaccgggct gttgctggcg atgttaatgc gcctgttact gctggcgtca atctcctggc
      421 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
      481 taatcatgct gtttggtggt ttcttcttgt tgttcaaagc cacgatggag ctgaacgaac
      541 ggctggaagg gaaagacagc aataacccca cgcaacgcaa aggtgcgaag ttctgggggg
      601 tggtgacaca aattgtggta ctggacgcca tcttctcact cgactcggtg attacagccg
      661 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
      721 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
      781 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
      841 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcgctca
      901 atcagttggc tatctttaac cggcgacgtt ttctttccgc taaccagacg ctgcgccagc
      961 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     1021 ccgaaaccgc gtctatgctg gtggatcatg gtaaccagca gatctttaat ccgcaggaac
     1081 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtcagcagc attatgacgt
     1141 cgcgccacga tattgagcat atcgatctca acgcgccgga agacgagatc cgccagttgc
     1201 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     1261 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     1321 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttacctgctc
     1381 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggttcag
     1441 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     1501 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     1561 cgaatggtca tatgccgctg gaagatctgg tgcaatatgt gccgctaccg ctggatgaaa
     1621 aacgtgaata tcacaccatt gccgggctgt tgatggaata tttacagcgt attccaaaac
     1681 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     1741 gcgtgcagaa ggtgcagatt attccgctac gtaaggatgg cgagatggag tacgaggtgt
     1801 gatggtatcg gtcattttac ccgatgctgc gtaacgcctt ataacttcag caatgaagcg
     1861 ttgtctgtgg tacatggaca ttacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     1921 gtttcctgta ggccggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     1981 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     2041 cgatttaccg ccgggagccc gggttgccag gatggtggcg attgagccac cctggcacgt
     2101 tcaccggctt gagcgttcca tagtagtgag gaacatcaag tgaacggaac agccttcgat
     2161 cttacatatt cagaatataa tggtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     2221 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     2281 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     2341 ttcgtcctgt agatgtttgc gcagtatctg atccacttgc agactgtaat tgagttgctg
     2401 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
     2461 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
     2521 cagattcaac atgccttccc ctgtaagcgc cagcacaggc gattgacctt gcatgtcgtt
     2581 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gacgcgtcac
     2641 gttatcgaag ttttcagcgg ccttcacatc accaccatta cgttctaccg cttgctgaat
     2701 catctgctgg aagttcatcc cttccatgcg cgtatcggcc atttcgacat gcgccgtccc
     2761 ctgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
     2821 tgacatcttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg tgccaatctc
     2881 aacgttttcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
     2941 gcccggcagt gaaacctgtc caccgttaag tttgccctgc agttgagtaa tttccagcaa
     3001 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
     3061 gttactggcc tgcaacaaaa tatcagccgt aaagccttgc agtccctgat aggccggttc
     3121 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
     3181 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
     3241 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgccc
     3301 gctcagcgta ctatcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
     3361 ctcctgccac tgggcctgga aactcccctg cccctgaata ccttgttttg gtaaatctgc
     3421 accctgcaac tgccagttaa tttgttcaat agccgccgtt aaatcatgcg gataatcaga
     3481 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
     3541 ggaaaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc gaatattgcg
     3601 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
     3661 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
     3721 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
     3781 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
     3841 cacgtccagg cgcatgttgt cggcgcgaac cagtggctgg ctcgcgccct gggcggtgag
     3901 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
     3961 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
     4021 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaggagtat
     4081 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
     4141 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
     4201 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
     4261 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc agccagacca
     4321 gcccggatcg atgcggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
     4381 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc
     4441 cagcgccagc tctcctgggt gaagatagaa cgcctcgctt tcatcgagaa cgatctcatc
     4501 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
     4561 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
     4621 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
     4681 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcgc atcactccct
     4741 taacgccaat tacgtcaagg gcataccagg ttatcactga acggtacaca atttatcaag
     4801 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
     4861 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
     4921 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
     4981 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
     5041 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
     5101 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
     5161 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcagcaggc
     5221 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
     5281 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
     5341 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
     5401 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
     5461 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
     5521 tccgcctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
     5581 ttcccggtgg caaattacgt tgatcagttt tatacaaagt aaaaaatgtt atacgcagtt
     5641 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
     5701 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
     5761 atgagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
     5821 agtttaggtt tagtctcgtt tatctttacc cttttctcgc ttgagctttc gcagtttggc
     5881 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
     5941 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
     6001 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
     6061 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
     6121 ctggctgact ggctgcgtct ggcactcggc agcgccattg ttccgcctct gttggggggt
     6181 gttttggttg tcctgctgac gcccggagac gatcctctca gggcattttt gatatgggta
     6241 ctgtcagaat ccatcggcgc tctggcactg gtgccgctgg gattgttatt taaaccacac
     6301 tatctgctgc gccatcgcaa cccaaggttg ctttttgagt cgctgctcac gttagccatc
     6361 acactgacgt taagctggct ttcgatgctg tatctgccgt ggccttttac tttcattatt
     6421 gtgctgttga tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt gatcttcctt
     6481 accacggtga tgatggtgtc gctgatgatg gccgcggatc cctccctgct tgctacgccg
     6541 cgtacgtacc tgatgagcca tatgccgtgg ctaccgtttt tgctgatcct gctgcccgcc
     6601 aacatcatga cgatggtgat gtatgccttt cgtgcggaac gcaaacacat ttccgaaagc
     6661 gaaacccgct ttcggaacgc gatggaatat tccgctatcg gtatggcgtt agtgggcacc
     6721 gagggacaat ggctgcaatc caacaaagca ctctgccagt ttctcggtta cagtcaggaa
     6781 gagctgcgcg gactcacctt tcagcaactg acctggccgg aggatctcaa taaagatctc
     6841 caacaggttg aaaagctgat aagcggtgaa ataaacacct attcaatgga aaaacgttac
     6901 tacaaccgca atggcgatgt tgtctgggcg ttgcttgccg tctctctggt gcgccacacg
     6961 gatggcacgc cgctctattt tatcgctcag attgaagaca ttaacgagct aaaacgcacc
     7021 gaacaggtga atcagcaact gatggagcgc atcacgctgg ctaacgaagc gggcgggatt
     7081 ggcatctggg agtgggagct gaagccgaat atttttagct gggataagcg gatgttcgaa
     7141 ctgtatgaaa ttcctccgca tatcaaaccg aactggcagg tgtggtacga gtgcgtgctg
     7201 ccggaagatc gtcaacacgc cgaaaaagtg attcgtgatt cgttgcaatc acgctcgccc
     7261 tttaagctgg aatttcgcat tgccgtgaaa gatggcatcc gccatatccg cgccctcgcc
     7321 aaccgggtac tgaataaaga aggcgaagtc gaacgcctgc tcggcattaa tatggatatg
     7381 accgaggtta aacagcttaa cgaggcattg tttcaggaaa aagagcgcct gcacattacg
     7441 ctggactcca ttggcgaagc cgtagtctgt attgatatgg cgatgaaaat tacctttatg
     7501 aatccggtgg cggagaagat gagcggctgg acgcaggaag aagcattagg tgttccgctc
     7561 ctgacggtgt tgcatattac ttttggcgac aacgggccat taatggagaa tatttacagt
     7621 gccgacacct cacgttccgc gatcgaacaa gatgtggtgt tgcactgtcg gagcggcggc
     7681 agctacgacg tgcattacag tattacgccg ttaagtactc tggacggcag caatattggt
     7741 tcggttctgg tgattcagga cgttaccgaa tcacgcaaaa tgctgcgcca gctgagctac
     7801 agcgcctccc atgatgcact gacgcatctc gccaaccgcg ccagttttga gaaacaactg
     7861 cgtatcctgc tgcaaacggt aaacagtacg catcagcgac atgcactggt gtttatcgat
     7921 cttgatcgct ttaaagcggt gaatgacagc gccgggcatg cggcgggcga cgctttgctg
     7981 cgcgaactgg cgtcgttgat gctgagtatg ctgcgctcca gtgacgtgct ggcgcggctc
     8041 ggcggtgatg aatttggtct gctgctgcca gactgcaatg ttgaaagcgc tcgttttatc
     8101 gctacacgta ttatcagtgc tgtgaatgac tatcacttta tctgggaagg ccgggtacat
     8161 cgggtaggtg ccagcgccgg gattaccttg atcgatgaca acaatcatca ggcggctgaa
     8221 gtgatgtcgc aggctgatat cgcctgttat gcctccaaaa atggtggccg gggccgggtg
     8281 acggtttacg aaccgcagca agctgccgca catagcgaac gggcggcgat gtcgcttgat
     8341 gaacagtggc ggatgattaa agagaatcag ttgatgatga tcgcccacgg tgtcgcttcg
     8401 ccacggatcc cggaagcgcg taatttgtgg ctgatttcac ttaagctctg gagttgcgaa
     8461 ggcgagatta ttgatgaaca aacatttcgt cgtagcttca gcgatccggc gcttagccat
     8521 gctcttgacc gccgggtatt ccacgaattt ttccagcagg ccgcaaaagc ggttgccagt
     8581 aaaggcataa gcatcgctct ccccctttcc gttgccggtt tgagtagcgc cacgctggtg
     8641 aatgatctgc ttgagcagct ggaaaatagc cctctaccac cacggttatt acatctgatt
     8701 attccggctg aagcgatttt agatcacgca gaaagcgtgc aaaaactgcg gctggcggga
     8761 tgtcggatag tgctcagcca ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg
     8821 aatatggcag attacctgct acttgatggt gagttatgcg ccaacgtgca gggtaatttg
     8881 atggatgaga tgctgattac gattattcag gggcacgctc agcgactcgg gatgaaaacc
     8941 atcgccgggc cagtcgtttt acccttagtg atggatacgc tttctggcat cggcgtcgat
     9001 ctgatttatg gtgatgtgat tgccgatgcc caaccgctgg atttgctggt gaatagtagt
     9061 tatttcgcga ttaactgagg cgtgcttccc catcgcctga tgcgacgcta acgcgtctta
     9121 tcatgcctac aaatcgctca ttccccaggc cggataaggt gctcgcaccg catccggcga
     9181 ccaacgtcat gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata
     9241 agaacgccag ggcttccagc gttcggcata acggcggatt tgtgccggtg tcattcccgg
     9301 aaatcgctgt ttaatcagat aatcatccgg caggaaaaca tctttcgcct gccagccacg
     9361 caaagcgaaa taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat
     9421 cgcctgctcc acatcgcccg gaattgccat tggtaagctg ccctccagcg ccgcatttgc
     9481 cagatgaatc agtgcctctg cccgtttcaa cgacatgcct aacgctttta atgcctgcgg
     9541 gtcggctgct gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag
     9601 ccgttcgcca taaagctgta ccactttggc agtcaatttt gccgccatcg ccacgctcac
     9661 cagttggcct aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg
     9721 caatccgggc cgcgccgcgc ctaacttgcc caacgcaccg ttaacaattt gtgggttaca
     9781 ttgcagatca aacaggcggc tcattttcgc cagacactct gcggcaacag gttctaaacc
     9841 tgcacttaaa tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcactacgcc
     9901 gcgatattcg cccaccgcca gactacgggc ataataactg tccgcgaccg tttccacacc
     9961 gctcaccgca cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca
    10021 gttcagggta tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac
    10081 gctgcgcttt gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc
    10141 ccttcttcac cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa
    10201 ctgttcagtg gcggtcatgc gtgacggtaa accgcacttg ctaaaaatgg aaaacgacag
    10261 cacgctgctg ccttcaatgc tttgcgcgcc aacgcgtgag gcggtaagcg aatggctgta
    10321 ccgccatcat gatgttccgg cagacgacga tgaaacgcag gcgctgttgc gtcgggcgat
    10381 tcgttataac cgcgaagaag atatcgatgt tacggcgaaa agcgtgcagt tcggtctttc
    10441 ctcactggca cagtacattg atgatccgga agaagtgtgg tttgtgaaat caccaaaatc
    10501 gttcctcggt gccagcggct taaaaccgca gcgggtagcg ctgtttgagg atctggtctg
    10561 cgcaatgatg ttgcacattc gccagcaggc gcaggcgcag ctgccagaag cgattactca
    10621 ggcggtgatt ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca
    10681 ggcgcaaggg attctggaac gcgcggcgaa gcgtgccgga ttcagggacg tggtattcca
    10741 gtacgagccg gtagcggctg ggctggatta cgaagccacc ttacaggaag aaaaacgggt
    10801 actggtggtg gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca
    10861 gtggcgttcg cgtctcgatc gtgaagccag cctgctgggt cacagtggtt gccgtattgg
    10921 cggtaacgat ctggatatcg cactggcgtt taaaaacctg atgccgttac ttggcatggg
    10981 tggcgaaacc gaaaaaggca tcgccctgcc gattctgccg tggtggaatg cggttgccat
    11041 caacgacgtt cctgcgcaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga
    11101 tctggtacgc gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca
    11161 gcgtttaagc tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt
    11221 agcggaaacc cgcgcctcac tgccgtttat cagcgatgaa ctggctacgc tgattagcca
    11281 gcaagggctg gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact
    11341 ggccctcgat aacgcccagg aaaaaccgga cgttatctat ctgaccggcg gtagcgcccg
    11401 ttctccgctg attaaaaaag cgctggaaga acagttgccg ggcattccga ttgcaggcgg
    11461 cgatgacttt ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tgtttcgtta
    11521 attgaaaaat ggctcagcag cggcttaaat ctgcctgcgc ctgagccagt ttttctgccg
    11581 cctgacgtaa cagttccatc aacggcatgg tccgtgctgg agcatgaagg cggaatcgct
    11641 gcaactctgt cgcgccgaca gtcaacgccg attccagccg atgtcgtttg gcggccattt
    11701 tcgccattac cgcttgtctg tctgcaggcg ttaccgcttc gttcggcctg aacacaaaac
    11761 ggcgttcaca gctcgctttc cagtcgatga ccgcctgggt cagatgatca ccaaaccctt
    11821 taacttgctt aaccccacga cgggtaacat ccgctgctgt ttcaatacca aaggaccgta
    11881 acgccgcttt acgcgcaggg ccaacaccgg gaatagaggc aacatcaata aaaaatccct
    11941 ccagaaactt ctgcttctgc cgttcccttg cagtgtcgtg aagtgctgcc agagcacgtt
    12001 tttcttcttc cggtaacccg agcatttcgt ccttcatttt ttcgagcatc gtccgtttgg
    12061 caataaaacc ttccagccca cccaactgtt ggatctggct gactaaatgg tcatagtcca
    12121 ttttcgtgcg attaaatgcc tcacggcgtt gctggatttc tgctttataa gctttgcttg
    12181 tcagactgcc aataatccag atagccgcca gcacaggaac caaaataata taacgcggtt
    12241 ctgcctgaag gccgcaaagc agcagcgata ccgctgacag tgcgatctca agcagaatga
    12301 tgtattcgcg ccgtaacagg cctaaaggaa gcggcctgcc agtcggttgg aaatgatcgg
    12361 gtaatggcaa ttgcactgct ggcggtgcta ctgacgccat caccatcgcc cagactttcg
    12421 ccagcacaaa atccccgccg gtggtaataa cctcttcgcc gagatcaata aaatagataa
    12481 cgccttgatt atccagcgta caccacgggc agtcagtcaa atgagcgggg taaacatgca
    12541 ttgctgaaac ggtacatttc tttaactgtt ggcgtagaga gtccagtgcc gctacccacg
    12601 cttttgccgt cggacgcccc gttgccacgc cactttccgt gaatgcctgc tgaaacatgg
    12661 cttcaacatc tcccggtaac atcgacagcg gaatagatcg tggcggcggt tttaaaccac
    12721 gtcgctgatt atctgacgcg taggcataac ggaaatgggc aatatccgtc tccagcgcat
    12781 tacccgcatc agagataagc ggcacaccag aataaggatg ccgcccacca aacaagacgt
    12841 gaaaaatcag caacgcaagg ccaaaattat cgtgattcgc ggtacgttca aaaccgacaa
    12901 atgatggcaa ggtttgtagc tctggcggcg taaaatgcga cacgccgact tcgcataaat
    12961 gcagtgtgcc attggcgtta atctgaaagg agtcactatc gatcaacacc actttgctgt
    13021 cgcgacctac cataaagctg ttctggttta cgtcacccac gacgtgcccg tgctcgtgaa
    13081 ccgtagcaaa agatgaagca atattgcgcg caacatagag tagaaaatcc cacgcacaat
    13141 gagggtaact ctggcgacga tgtgccgggc tatagatcat atgaatcggt tctttaccag
    13201 aaacttttgg catcataaaa ccgataactt ttccgcctcg tccaccgtga agcgttgcct
    13261 gcggccaggc gacataattc aacaactgcg cgtcagctgt cgcagccata aaggcaagtt
    13321 tgtcctgttt taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta tcggcaaact
    13381 cattgatatc ataaaccgca ccttcaccgc ctttgcccag ttcacggcct gtcgtaacgc
    13441 actcaccagt agcagtatat aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt
    13501 tttatcatca tcggtacgct cgtttaccgc cgggctggac aaaaactgct ttaacaattc
    13561 aggtaataaa tcgagctgtt cctgcgttgc tgccgccagt ccattgaaaa aaggggtaaa
    13621 gaaaggcaca tgcggagaat tatccaacat atttaacgcc agtcgctgga taccatccgt
    13681 aaatgctgca actttatgca cacgctcagt gctggtgaaa gtttccagtc tggaaacggc
    13741 atcttcatcg gtaatgaaat gcgtcatatt ggcatattca cccgccatcg gcgtaagcgg
    13801 taattgcaga ccgtgaccaa aatccaccac cacgccgcca tcgccaatct gcataatcaa
    13861 ggtgccgtta gccgatgaaa tcaatcccag aaacgtgcag gcaaaatcgc gaactgctaa
    13921 ttctttggct tccgcctcgg caaaaagtcg ctgacgaacg gtcagcacaa tatccgtggc
    13981 gaggatatca ttgagtccaa attcaccgtg ctgcactttt tgcgacacat aagccatcgc
    14041 ttcattgatg gcaagcatcg cgccttcgcc accctgcgag acactgcctg cgccatcagc
    14101 gacaaatacc gacaataatg gctgctggtc atttagccag gcaacctgca tctggcaggc
    14161 atcctggcag ggtaaatcag cgctaatgtg cgacgttcca acagcggatg cataaaccag
    14221 acgccagctc acactgacgt ccagcctttt ggcgcttcca gtacaacttc cgttcccggc
    14281 gtggagcggg aaaccgaacg cagtgaactg gataaccagc taaacagttc gcggaattgt
    14341 aatccctgca gagacaaagg ctgacgaacg ctgatttgtg ccagagtctt catatccgcc
    14401 ccctgaacac cgatggaaaa gaaggcaaac ttcttatctt cttccccctg aaacactttg
    14461 ttggcggcag cctgccactc atcggttgga gcgccatcgg tgatcaggaa aatccacggg
    14521 cgataatagg aaataccatt tgcgcggtac tcacgtttcc gctcctctac catatccagg
    14581 gctttggtaa tggcggcacc cattggtgta tcgccctggg caaacaggat gggcgggaaa
    14641 aaattagcgg cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatacca
    14701 agttccactc tttttaaggc cagcgggtcg gcaagcagct catcgcgaaa ggtaaccaat
    14761 ccggcgttaa gttcattaat gggtctgcca ctcatcgaac cagaaacatc cagcagcaga
    14821 atgcaggggc aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt
    14881 tcgctcataa gaaatccttt cttagagaat tattgaggtc cggtatcata ctgattgcaa
    14941 taatgaaata agagcaaacg aagaaacttt cagaattagt cccggctgcg cagaaaattt
    15001 tgctattcgc ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa
    15061 tttcaggagt gagggttagg gagaggtttc cccctccccc tggtgttctt agtaagcctg
    15121 gaagctaatc actaagagta tcaccagtat gatgacgtgc ttcatcataa ccctttcctt
    15181 attaaaagcc ctcttctccg ggagagggtt tcccgtttca gcgtcccgct gaaatcgtcg
    15241 gcttacctcc tttcgccctg aatgcagtct atcgctaacg cgtgaacaaa atagttccct
    15301 ctgtgattat tgcgtaacga ctcgcaaaga atatttgctg gcaggatcgc agactgcaaa
    15361 gcctgcagat tgacaatctt atcgtgaagg catactttca ggagtaaggg tagagcgggg
    15421 tttcccccgc cctggtagtc ttagtaagcc gggaagctta tgactaagag caccacgatg
    15481 atgatccact tcatcatgac cctttcctta tttaaggccc cttcctcggg aggggctttc
    15541 ccgtttcagc gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa ttcagtctat
    15601 cgctaacgcg tgaacaaaat agtttcctct gtgattattg cgtaacgtct cgcaaagaat
    15661 atttgctggc aggatcgcag actgcaaagc ctgcggattg acattctgaa cgtgaaggca
    15721 tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtttt agtatgctgg
    15781 gtagctaatc actaagagca ccacgatgat gagtatcttc atcatgaccc tttccttatt
    15841 taagacccct tcctcgggag gggctttccc gtttcagcat cccgctgaaa tcgtcggctt
    15901 acctcctttc gccatgcatg cagtctatcg ctaacgcgta gataaaatag tttcctgtgt
    15961 tattactgga tgcgtgctcg caaatgtgcc cgccattcag acgattcccg acagtgtttc
    16021 ataattcctc catttttctc ccttattggc tggctacact agtatcattc cgcgaaacgt
    16081 ttcaggaaga gaaactctta acgatgaaag gcagttataa atcccgttgg gtaatcgtaa
    16141 tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc aatgaacccc
    16201 agagcgcagc accaggtgcg acgaaacaag cgcagcaccc acctgcgggg gggcgccgtg
    16261 gtatgcgttc tggcccttta gcgcctgttc aggcagcgac tgccgtggaa caggcggttc
    16321 cgcgttatct caccgggctt ggcaccatca ccgcggctaa caccgttacg gtgcgcagtc
    16381 gcgtggacgg ccaactgatg gcgttgcatt tccaggaagg ccagcaggtc aaagcaggcg
    16441 atttactggc agaaattgac cccagccagt ttaaagttgc attagcacaa gcccaggggc
    16501 aactggcaaa agataaagcc acgcttgcca atgcccgccg tgatctggcg cgttatcagc
    16561 aactggcaaa aaccaatctc gtatctcgtc aggaactgga tgcccaacag gcgctggtca
    16621 gtgaaaccga aggcaccatt aaagccgatg aagcgagcgt cgccagcgca caactgcaac
    16681 tcgactggag ccggattact gcgccagtcg atggtcgtgt tggcctgaag caggttgatg
    16741 ttggtaacca aatctccagt ggcgacacga ccgggattgt tgtgatcacc cagacgcatc
    16801 ctatcgattt gctctttact ctgccggaaa gcgatatcgc taccgtagtg caggcgcaaa
    16861 aagccggaaa accgctggtg gtagaagcct gggatcgcac caactcgaag aaattaagcg
    16921 aaggcacgct gttaagtctg gataaccaaa tcgatgccac taccggtacg attaaagtga
    16981 aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt aacgcgcgca
    17041 tgttagtcga caccgaacaa aacgccgtgg tgatccccac cgccgccctg caaatgggca
    17101 acgaaggcca ttttgtctgg gtactgaata gcgaaaacaa agtcagcaaa cacctggtga
    17161 cgcctggcat tcaggacagc cagaaagtgg tgatccgcgc aggtatttct gcgggcgatc
    17221 gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa gtggtagaag
    17281 cccagagcgc caccgcaccg gaagagaaag ccaccagccg cgaatacgcg aaaaaaggag
    17341 cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc gcctgtttat
    17401 tatgcgtcct gtggccacca cgctgctgat ggtggcgatc ttactcgccg ggattatcgg
    17461 ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca ttcaggtggt
    17521 cacgctctac ccaggtgcga gcccggatgt catgacctct gccgttaccg cgccgctcga
    17581 acgccagttc gggcagatgt ctggtctgaa acagatgtcg tcgcaaagtt ccggcggtgc
    17641 gtcagttatc actttgcagt tccagctaac attgccgctg gatgtcgccg agcaggaagt
    17701 tcaggccgcg attaacgccg ccactaacct gctacctagc gatctgccta acccgccggt
    17761 ttacagcaaa gtgaacccgg cagatccgcc gattatgacg ctcgccgtca cctcaaccgc
    17821 catgccgatg acccaggtag aagatatggt ggaaacccgc gtcgcgcaga agatctcgca
    17881 gatttccggc gtcggcctgg tgacactttc cggcggccag cgtccggctg ttcgcgtcaa
    17941 acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc gcaccgccat
    18001 taccggtgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc gcgcggtcac
    18061 gctttccgcg aatgaccaga tgcaatccgc cgaagagtat cgccagttga ttatcgccta
    18121 ccagaacggc gcgccgattc gtctgggcga tgtcgcaacc gtagagcaag gggcagaaaa
    18181 cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg ttcagcgcca
    18241 gcccggtgct aacattatct ccaccgccga cagcatccgg cagatgctgc cgcagcttac
    18301 cgagagtctg ccgaaatcgg tgaaggtgac ggtgctttct gaccgcacca ccaatatccg
    18361 agcatccgtc gacgataccc agtttgaatt gatgatggct atcgcgctgg tagtcatgat
    18421 tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgtcg ctgtgccgct
    18481 gtcattaatc ggcaccttcg cggttatggt gtttctcgat ttttcaatca ataacctgac
    18541 gctgatggcg ttaactatcg ccaccgggtt cgtggtcgat gacgccatcg tagttatcga
    18601 aaatatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc tcaaaggcgc
    18661 gggtgaaatc ggctttacca ttatctcgct gaccttctca ctgattgcgg tgttgatccc
    18721 actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta ttaccctggc
    18781 ggtagcgatt ttaatctcag ccgtggtgtc gctaaccctg acaccgatga tgtgcgcacg
    18841 gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct cggaaaaaat
    18901 attcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga atcatccgtg
    18961 gctgacctta agcgtggcgc tcagcacgct gctgcttagc gttctattgt gggtgttcat
    19021 tccgaaaggt ttcttcccgg tacaggataa cggcattatt cagggcactt tgcaggcacc
    19081 gcaatccagc tcctttgcca atatggccca gcggcaacgc caggtcgcgg acgtgatttt
    19141 gcaggatccg gcagtgcaaa gtctgacctc atttgttggc gttgatggca ctaacccgtc
    19201 gctgaacagt gcgcgtttgc agatcaacct caaaccgttg gatgaacgtg atgatcgggt
    19261 gcaaaaagtc atcgcccgtc tgcaaacggc ggtggataaa gtgccgggcg tcgatctctt
    19321 cctgcaacca acgcaggacc tgaccatcga tactcaggtc agccgcaccc agtaccagtt
    19381 taccttgcag gccacgtcac tggatgcgct cagtacctgg gtgccacagt tgatggaaaa
    19441 actccagcag ctgccacagc tttctgatgt ctccagcgac tggcaggaca aagggctggt
    19501 ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca tggcggacgt
    19561 cgataacgcc ctgtacaacg cgtttggtca gcggctgatt tccactattt atactcaggc
    19621 taaccagtat cgcgtggtgc tggagcacaa caccgaaaat accccaggcc tcgcggcgct
    19681 ggataccatt cgcctgacca gcagcgacgg cggcgtggtg ccgctaagct caattgccaa
    19741 aattgagcag cgttttgcgc cgctttccat caaccatctg gatcagttcc cggtaacgac
    19801 catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg caattatgga
    19861 caccgagaag acgctgaatc tcccggtgga tatcaccacg caattccagg gcagcaccct
    19921 cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg tggtggcgat
    19981 gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca ttctctcgac
    20041 gctacccacc gcaggggtcg gcgcactgct ggcattaatg cttgccggaa gtgagctgga
    20101 tgtgattgcg attatcggca ttattttgct gatcggtatc gtgaagaaga acgccatcat
    20161 gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc gcgatgcaat
    20221 ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg cggctctgct
    20281 tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc gtccgttagg
    20341 tatcggcatg gtcggcggcc tgattgtcag ccaggtgctg acgctgttca ccacgccggt
    20401 gatttatctg ctgtttgacc gcctggcatt gtggaccaaa agtcgctttg ctcgccatga
    20461 agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg cgacgatttt
    20521 actgtcggtt gccattaccc tgtgcggcat actgggtttc cgtatgctgc cggtcgcccc
    20581 gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg gtgcatcgcc
    20641 ggagacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc gcattgccgg
    20701 ggtcagtgaa atgacctcca gtagttcgct cggcagcacg cgcattattt tgcagtttga
    20761 ttttgaccgg gatatcaacg gcgcagcgcg tgatgtgcag gcggcgatca acgctgcaca
    20821 aagtttgctg cccagtggga tgcccagccg tccgacctat cgcaaagcga acccgtcgga
    20881 tgcgccaatt atgatcctga cgctaacctc cgatacttat tcgcagggtg aattgtacga
    20941 tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggcgtcg gtgatgtcga
    21001 tgtcggcggc agctcgctgc ccgccgtgcg cgtcgggctg aacccgcagg cgctgtttaa
    21061 tcagggcgtg tcgctggacg acgtacgcac tgccatcagc aatgccaacg tgcgtaaacc
    21121 gcagggggcg ctggaagatg gcactcaccg ctggcagatc cagaccaacg atgaactgaa
    21181 aaccgccgct gaatatcagc cgctgattat tcactacaac aacggcggcg cggttcgtct
    21241 gggcgatgtg gcgacggtga ccgactcagt gcaggatgtg cgcaacgccg ggatgaccaa
    21301 cgccaaaccg gctattttgc tgatgatccg caagctgccg gaagccaata ttatccagac
    21361 ggttgacagc atccgggcaa aattaccgga gttgcaggaa accattccgg cggcgattga
    21421 tctgcaaatt gctcaggatc gctcccccac cattcgcgcc tcgctggaag aagtcgagca
    21481 aacgctgatt atctcggtgg cgctggtgat tctggtggtc tttttattcc tgcgctcggg
    21541 tcgcgccact attattcccg ccgttgcggt gccggtttcg ctgattggta cgtttgcggc
    21601 gatgtacctg tgcggattca gtctcaataa cctgtcgtta atggcgctca ccatcgctac
    21661 tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcccgtc atctggaagc
    21721 ggggatgaaa ccactacagg ccgcgctaca aggtactcgc gaagtcggct ttacggtgct
    21781 gtcgatgagt ctgtcactgg tggcggtgtt cctaccgctg ctgttgatgg gcggattgcc
    21841 gggccgactg ttacgcgagt ttgccgtgac gctttctgtc gccattggta tttcgttgct
    21901 ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca gcaagccgcg
    21961 cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac aaggctatgg
    22021 taagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc tgcttggcac
    22081 cattgctttg aatatctggc tatatatctc gatcccgaaa accttcttcc cggagcagga
    22141 cactggagtg ttgatgggcg ggatacaggc ggatcagagt atttcgtttc aggcgatgcg
    22201 cggtaagttg caggatttta tgaaaattat ccgtgacgat ccggcagtgg ataatgtcac
    22261 cggctttacc ggcggttcac gagttaacag tgggatgatg tttatcaccc tcaagccacg
    22321 cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgggtga agctggcgaa
    22381 agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg gcggacgtca
    22441 gtctaacgcc agttatcagt acacgttgtt atccgacgac ctggcggcac tgcgcgaatg
    22501 ggagccgaaa atccgcaaaa aactggcgac attgccggaa ctggcggacg tgaactccga
    22561 ccagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca tggcacggct
    22621 gggaatcgac gtacaggccg ccaacagcct gttaaataac gccttcggtc agcggcaaat
    22681 ctcgaccatt taccagccga tgaaccagta caaagtggtg atggaagtgg atccgcgcta
    22741 tacccaggac atcagcgcgc tggaaaaaat gttcgttatc aataacgaag gaaaagcgat
    22801 cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg tgaatcatca
    22861 gggattatcg gcagcctcga ccatctcgtt caacctgccg accggaaaat cgctctcgga
    22921 cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga cggtgcgcgg
    22981 cagttttgcc ggcacggcgc aggtgttcca ggagacgatg aactcacagg tgatcctgat
    23041 tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga gttatgtaca
    23101 tccgctgacg attctctcca ccctgccctc ggcgggcgtt ggggcgctgc tggcgctgga
    23161 gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat taatcggcat
    23221 tgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac ggcacggtaa
    23281 cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc cgattatgat
    23341 gactaccctg gcggcgctgt ttggcgcgtt gccgctggtg ttgtcgggcg gcgacggctc
    23401 ggagctgcgc caacccctgg ggatcaccat tgtcggcggt ctggtaatga gccagctcct
    23461 gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctacggc tgcgtttttc
    23521 gcgtaaacct aaacaaacgg taaccgagta aatgacagat cttcccgaca gcacccgctg
    23581 gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca ccatcgttaa
    23641 caccgcgctt ccctcaatgg cgcaaagcct gggggaaagt ccgttgcata tgcacatggt
    23701 tattgtctct tatgtgctga ccgtggcggt gatgctgcct gccagcggct ggctggcgga
    23761 caaagtcggc gtgcgcaata ttttctttac cgctatcgtg ctgtttactc tcggttcact
    23821 gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt tacagggcgt
    23881 tggcggtgcg atgatggtgc cgatcggaag gcttacggtg atgaaaatcg taccgcgcga
    23941 gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc cgctgctcgg
    24001 tccggcgctt ggcggcctgc tggtggagta cgcgtcgtgg cactggatct ttttgatcaa
    24061 cattccggtg ggaattattg gtgcgatcgc cacattgatg ttaatgccga actataccat
    24121 gcagacgcgg cgctttgatc tctccggctt tctgttgctg gcggttggca tggcggtatt
    24181 aactctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga ttgctggcct
    24241 ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa ataacaaccg
    24301 cgctcttttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc tggcggggag
    24361 ctttgccgga cgtatcggta gcggcatgtt gccctttatg acgccggttt tcctgcaaat
    24421 tggccttggc ttctcaccgt ttcatgccgg actgatgatg atcccgatgg tgcttggcag
    24481 catgggaatg aagcgaattg tggtacaggt ggtgaatcgc tttggttatc gtcgggtact
    24541 ggtagcgacc acgctgggtc tgtcgctggt caccctgttg tttatgacta ccgctctgtt
    24601 gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca actcgacccg
    24661 tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg cgagcagcgg
    24721 caacagtctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca ccatcgccgg
    24781 gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca ccacacaaac
    24841 cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg cgttcatctt
    24901 tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa aaaggagcgc
    24961 gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat tttcgccacc
    25021 tgcattgtct tgctgatcag tatgcactgg gcggtgcgta ttagttttga gcgtggcttt
    25081 attgattaca tcaagcatgg taatgaacag cgattacaac tgttaagtga tgcgcttggc
    25141 gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg ctttgtcttt
    25201 cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc gggtatgcca
    25261 ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt gctggttggt
    25321 ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa cggtgcggaa
    25381 gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga tatcaatttc
    25441 gataaacaac agcggcaaac cagctggctg attgtcgccc tggcaacgtt actcgcggcg
    25501 ctcgccacct ttctgctggc gcgtggtctt ttggccccgg tcaaacggct ggtggacggc
    25561 acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag tgaagatgaa
    25621 ctgggcaaac tggcgcaaga cttcaaccag cttgccagca cgctggagaa aaaccagcaa
    25681 atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt agcggtgctg
    25741 cgcggtgaac tagaagctat tcaggatggc gtgcgcaaat tcacgccgga gacggtggcg
    25801 tctttacagg cggaggtcgg tacactgacc aaactggttg acgatcttca tcagttgtcg
    25861 atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat cccactgctg
    25921 gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa actgcaattt
    25981 tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca gttgttcaat
    26041 aacttactgg aaaacagcct gcgctacact gacagcggcg gcagcctgaa aatctctgcc
    26101 gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg tgtcagtgac
    26161 gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg caaccgtgcc
    26221 agcggcggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc acataatggc
    26281 cgcattattg ccgcccattc gccttttggc ggggtaagca ttacagtaga gttaccgctg
    26341 gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac acaccgcgta
    26401 ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat ctgcgtgctg
    26461 cgagctatgc gccgacgctt atcagtcacg gcgatcaggt actgccgtat gtgcgccaga
    26521 caccaccgga tctgatcctg ttggatctga tgctccctgg caccgatggc ctgacgctgt
    26581 gccgggaaat tcgtcgtttt tctgacattc ctatcgtgat ggtgacagca aaaatcgaag
    26641 agatcgaccg cctgctgggg ctggagattg gcgcagatga ctacatctgc aagccgtaca
    26701 gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg ccgttgcaaa ccgcagcgtg
    26761 agttgcaaca acaggatgct gaaagcccgt tgattatcga cgaaggccgt tttcaggctt
    26821 catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgctg aaaacgctct
    26881 ctcatgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt tatgacgact
    26941 accgcgtagt aaccgaccgc accatcgaca gccacattaa aaacctgcgc cgcaagctgg
    27001 aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt taccgctggg
    27061 aagccgacgc ctgccgcatc gtttagtttt agcgacatga ctttgttagc cggagatggc
    27121 gttcagaccc gatccggcaa cattatccca cgcatggtca gcaaactgac attatctctc
    27181 cgccctggca ggcatcagaa aatcgctcat actttaatcg gtaaacagca cctttagatg
    27241 ctgttttcga tacacaattt caatcaagga gtcattatgg ctggttggtt tgaactcagt
    27301 aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg gcaatggtga gactatcctc
    27361 accagcgagc tgtatacttc aaaagcctct gcggaaaagg gcatcgcgtc ggtgcgtagc
    27421 aacagcccgc aagaagaacg ctatgagaaa aaaacggcaa gcaacggcaa attctatttc
    27481 aatctgaaag ccgccaatca tcaaattatc ggctccagcc agatgtatgc caccgcgcaa
    27541 tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca caagccagac ggtgaaagac
    27601 aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc ggctttattt ccaaataacg
    27661 cttatttatt tacctgcgcc attaaaatag tggacactga tgaatactca ttgtgctttc
    27721 tttgtttaac atctccatgc ttatcctcct gaacatgttc atctgccaaa atatcttcta
    27781 atacttttcg tgacgctatc cggtttatga catacactga tgtaagattt ttacttacag
    27841 gagagcataa acatggccag aacgatgacg gttgatctcg gcgatgagtt gcgtgaattt
    27901 atcgagtcct taattgaatc cggcgactat cgcacccaaa gcgaagtgat ccgtgagtca
    27961 ctgcgcttac ttcgagaaaa gcaagcagag tctcgcctgc aagcattacg tgacatgctt
    28021 gcagaagggt tgagtagtgg tgaagctcag ccgtgggaaa aggacgcgtt tttacggaag
    28081 gtgaaagcag ggataaggaa ataatgtgcg tattattaaa ctcatgccaa aagccaatga
    28141 ggatttagaa ggcatctggt attatagtta tcaccatttc ggcgaaccac aagccgacag
    28201 atatgtggaa catctttcag acgttctcca gattctgagt aacaataata taggtacgcc
    28261 cagacctgaa ttaggcgaag gtatttttgt tcttcctttt gaacgccacg taatttattt
    28321 cctgcaatca ccaggggaaa ttatcgtcat tcgtatcctt aaccaaaacc aggacgccac
    28381 cagacattta cactggagct aaacccgccg attgcgctac aatgcccgcc cttaaagtgg
    28441 gggcactccc ctaaccgctt catcaggtga agcggatctg acctgtcatc agaacgagag
    28501 aattatgttt aaaccggaac tcctttcccc ggcgggaacg ctgaaaaata tgcgttacgc
    28561 tttcgcttat ggcgcagatg ctgtttatgc gggccagccg cgttactccc tgcgtgtgcg
    28621 caacaacgaa ttcaaccacg aaaaccttca gctcggcatc aatgaagccc acgcgctggg
    28681 gaaaaagttt tatgtcgtgg tcaacattgc accgcacaac gccaagctga aaacctttat
    28741 ccgtgacctg aaaccagtgg tggaaatggg gccggatgcg ctgattatgt ccgatccagg
    28801 gctgattatg ctggtgcgtg agcacttccc tgaaatgccg atccatctct cggtacaggc
    28861 taacgccgtg aactgggcga cggtgaaatt ctggcagcaa atgggcctga cccgcgtgat
    28921 cctctctcgc gagctgtcgc tggaagagat tgaagagatc cgcaatcagg tgccggatat
    28981 ggagatcgag atcttcgttc acggcgcact gtgcatggcc tactccggtc gctgcctgct
    29041 ctctggctat atcaacaagc gcgatccgaa ccagggtacc tgcaccaacg cctgccgctg
    29101 ggagtacaac gtccaggaag ggaaagaaga tgacgttggc aacatcgtac acaagtacga
    29161 gccgattccg gtgcaaaatg ttgagccgac gctgggtatc ggcgcgccaa ccgacaaagt
    29221 gtttatgatc gaagaagccc agcgtccggg cgagtatatg accgcgtttg aagatgagca
    29281 cggcacttac atcatgaact cgaaagatct gcgcgctatc gcccatgttg aacgcctgac
    29341 caaaatgggc gtgcattcgc tgaaaatcga aggccgtacc aaatctttct actattgcgc
    29401 acgcaccgca caggtttacc gcaaagctat tgatgacgcc gctgcgggca aaccgtttga
    29461 caccagcctg ctggaaaccc tggaaggcct ggcgcatcgc ggttacaccg aaggattcct
    29521 gcgtcgtcat actcacgacg attatcagaa ctacgaatac ggttattcgg tttctgaccg
    29581 ccagcagttt gttggtgagt ttaccggtga gcgcaagggg gacctcgcgg cggtagcggt
    29641 gaaaaataaa ttctccgttg gcgacagcct tgagctgatg acgccgcaag gcaacattaa
    29701 ctttaccctt gagcacatgg aaaacgccaa aggtgaagcg atgccggtcg caccaggcga
    29761 tggttatact gtgtggctcc cggtgccgca ggatcttgag ctcaattacg cgctgctgat
    29821 gcgtaatttc tccggggaaa ccacgcgtaa cccccacggt aagtgattaa tttcgattat
    29881 ttttcccgga tggaaaattc ttagaaaccg atcacataca gctgcattta ttaaggttat
    29941 catccgtttc gctgaaaaac ataacccata aaatgctagc tgtaccagga accacctcct
    30001 tagcctgtgt aatctccctt acacgggctt attttttatg cataagccct atccctggtc
    30061 accgtcttcc attgaccaca tcgatagaat ctcccatcat agcacgatgc ctttcacgta
    30121 acggcatcgt gctcgcacag gttccggcta cgcacagcca gaacgcgcat gtttgacgct
    30181 taccaaaaaa tattctcact ctacacattt gaatgtcaga cgagcgacgc catgtaatcc
    30241 tgcaccttct gtcttcaggt caactatctg catttttttg ccctgagtaa cacagaaatg
    30301 ggctgcatca ttttttacta tattttctgc accagatatt ctccccctgg ctaaagaagc
    30361 ttcggcttcg gtgtagtatt ggttatcgag tttacgctga atattacttt tatatgcaag
    30421 accaaattta ccgatacttg tctcatcatt atgcacagca caaccagaca taagaaaaat
    30481 actaaataat gatatagcaa ctatcttttt catctcacct ttccccatta aataccaacg
    30541 acactctcta gtgtttaaat ataataatgg ctgattatta taattgaata ggattataat
    30601 aaatgttctg tacaacattt cctacataag t
//
LOCUS       DAZGBC010000045        30611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGBC010000045 DAZGBC010000000
VERSION     DAZGBC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.045"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..282
                     /locus_tag="ACHK1J_002008"
     CDS             94..282
                     /locus_tag="ACHK1J_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911280.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTRAVISGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            279..1106
                     /locus_tag="ACHK1J_002009"
     CDS             279..1106
                     /locus_tag="ACHK1J_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO7911281.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQAEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSDANTPFIQVK"
     gene            1147..1518
                     /locus_tag="ACHK1J_002010"
     CDS             1147..1518
                     /locus_tag="ACHK1J_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO7911282.1"
                     /translation="MNTHNAQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERTGGTDIMATINIGELICQDEDLSGESW
                     LAGMLVAAEIISIEPATNIRL"
     gene            1550..1792
                     /locus_tag="ACHK1J_002011"
     CDS             1550..1792
                     /locus_tag="ACHK1J_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7911283.1"
                     /translation="MFDLINQGQLYTDSAGYPVKIIHCINNIVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            1796..1930
                     /locus_tag="ACHK1J_002012"
     CDS             1796..1930
                     /locus_tag="ACHK1J_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911284.1"
                     /translation="MNKAFELWVRQCYGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            1949..2203
                     /gene="xisR"
                     /locus_tag="ACHK1J_002013"
     CDS             1949..2203
                     /gene="xisR"
                     /locus_tag="ACHK1J_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7911285.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            2237..3523
                     /locus_tag="ACHK1J_002014"
     CDS             2237..3523
                     /locus_tag="ACHK1J_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7911286.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            3525..4304
                     /gene="yecE"
                     /locus_tag="ACHK1J_002015"
     CDS             3525..4304
                     /gene="yecE"
                     /locus_tag="ACHK1J_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO7911287.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            4357..4752
                     /gene="yecN"
                     /locus_tag="ACHK1J_002016"
     CDS             4357..4752
                     /gene="yecN"
                     /locus_tag="ACHK1J_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO7911288.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            4793..5536
                     /gene="cmoA"
                     /locus_tag="ACHK1J_002017"
     CDS             4793..5536
                     /gene="cmoA"
                     /locus_tag="ACHK1J_002017"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO7911289.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            5533..6504
                     /gene="cmoB"
                     /locus_tag="ACHK1J_002018"
     CDS             5533..6504
                     /gene="cmoB"
                     /locus_tag="ACHK1J_002018"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO7911290.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(6669..9097)
                     /gene="torZ"
                     /locus_tag="ACHK1J_002019"
                     /pseudo
     CDS             complement(6669..9097)
                     /gene="torZ"
                     /locus_tag="ACHK1J_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(9122..10222)
                     /gene="torY"
                     /locus_tag="ACHK1J_002020"
     CDS             complement(9122..10222)
                     /gene="torY"
                     /locus_tag="ACHK1J_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO7911291.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAVGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(10610..11356)
                     /gene="cutC"
                     /locus_tag="ACHK1J_002021"
     CDS             complement(10610..11356)
                     /gene="cutC"
                     /locus_tag="ACHK1J_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO7911292.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(11370..11936)
                     /gene="yecM"
                     /locus_tag="ACHK1J_002022"
     CDS             complement(11370..11936)
                     /gene="yecM"
                     /locus_tag="ACHK1J_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7911293.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            12152..13885
                     /gene="argS"
                     /locus_tag="ACHK1J_002023"
     CDS             12152..13885
                     /gene="argS"
                     /locus_tag="ACHK1J_002023"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO7911294.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLATAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(13927..13971)
                     /locus_tag="ACHK1J_002024"
     CDS             complement(13927..13971)
                     /locus_tag="ACHK1J_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911295.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            14062..14550
                     /gene="yecT"
                     /locus_tag="ACHK1J_002025"
     CDS             14062..14550
                     /gene="yecT"
                     /locus_tag="ACHK1J_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO7911296.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(14672..15064)
                     /gene="flhe"
                     /locus_tag="ACHK1J_002026"
     CDS             complement(14672..15064)
                     /gene="flhe"
                     /locus_tag="ACHK1J_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO7911297.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(15064..17142)
                     /gene="flhA"
                     /locus_tag="ACHK1J_002027"
     CDS             complement(15064..17142)
                     /gene="flhA"
                     /locus_tag="ACHK1J_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO7911298.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(17135..18283)
                     /gene="flhB"
                     /locus_tag="ACHK1J_002028"
     CDS             complement(17135..18283)
                     /gene="flhB"
                     /locus_tag="ACHK1J_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO7911299.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(18485..19129)
                     /gene="cheZ"
                     /locus_tag="ACHK1J_002029"
     CDS             complement(18485..19129)
                     /gene="cheZ"
                     /locus_tag="ACHK1J_002029"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO7911300.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(19140..19529)
                     /gene="cheY"
                     /locus_tag="ACHK1J_002030"
     CDS             complement(19140..19529)
                     /gene="cheY"
                     /locus_tag="ACHK1J_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO7911301.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(19544..20593)
                     /gene="cheB"
                     /locus_tag="ACHK1J_002031"
     CDS             complement(19544..20593)
                     /gene="cheB"
                     /locus_tag="ACHK1J_002031"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO7911302.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(20596..21456)
                     /gene="cheR"
                     /locus_tag="ACHK1J_002032"
     CDS             complement(20596..21456)
                     /gene="cheR"
                     /locus_tag="ACHK1J_002032"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO7911303.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSREWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(21475..23076)
                     /gene="tap"
                     /locus_tag="ACHK1J_002033"
     CDS             complement(21475..23076)
                     /gene="tap"
                     /locus_tag="ACHK1J_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO7911304.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQVEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(23122..24783)
                     /gene="tar"
                     /locus_tag="ACHK1J_002034"
     CDS             complement(23122..24783)
                     /gene="tar"
                     /locus_tag="ACHK1J_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO7911305.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQSPAQPRLRIAEQDPNWETF"
     gene            complement(24926..25429)
                     /gene="cheW"
                     /locus_tag="ACHK1J_002035"
     CDS             complement(24926..25429)
                     /gene="cheW"
                     /locus_tag="ACHK1J_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO7911306.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(25450..27414)
                     /gene="cheA"
                     /locus_tag="ACHK1J_002036"
     CDS             complement(25450..27414)
                     /gene="cheA"
                     /locus_tag="ACHK1J_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO7911307.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFGVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(27419..28345)
                     /gene="motB"
                     /locus_tag="ACHK1J_002037"
     CDS             complement(27419..28345)
                     /gene="motB"
                     /locus_tag="ACHK1J_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO7911308.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGNRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(28342..29229)
                     /gene="motA"
                     /locus_tag="ACHK1J_002038"
     CDS             complement(28342..29229)
                     /gene="motA"
                     /locus_tag="ACHK1J_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO7911309.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(29356..29934)
                     /gene="flhC"
                     /locus_tag="ACHK1J_002039"
     CDS             complement(29356..29934)
                     /gene="flhC"
                     /locus_tag="ACHK1J_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO7911310.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(29937..30287)
                     /gene="flhD"
                     /locus_tag="ACHK1J_002040"
     CDS             complement(29937..30287)
                     /gene="flhD"
                     /locus_tag="ACHK1J_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO7911311.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         7496 a         8158 c         7396 g         7561 t
ORIGIN      
        1 tttaatcctt gccacagtac tgacactaac agaaatcatt attctcttta ttgcgctgtc
       61 tgtcagttaa aaatatcggg ataccacata ccaatgagac tgtatttcac aatagtaatt
      121 ttactggcaa ttatcgcatg catttacgga ttactcgttc cgttccttat atccatgaag
      181 gatacgagag cagttatttc tggctttgca ctggcgtttc tgaccccgcc ctgcatttat
      241 gccatttaca agggtctttc tttcactaag gataaaaaat gaaaaaaatt atttttgctt
      301 tagccattgt tctgccgacc attggtcttg tcggttgcga tcgcgttgag cccggtaatg
      361 ttggcatcaa agtaaataaa ctgggcgacg ataaaggcgt cggtgaggtg gtcggtgttg
      421 gtcgctactg gactggctgg aatactgaag tttacatctt ccccaccttc aagcaaatga
      481 agacctacga tgaaccgttc agctttcaga tgagtgacgg cacaaccatc ggctatcaca
      541 tcggtgtggc ctacaaggtt gatccatcca aagttactac agtgtttcaa acctaccgca
      601 aaggcgtgga tgacattacc gacactgacc tgcgccagaa gatagccgac gcactcaacc
      661 gactggccag caaaatgacc accgataaat ttatcgacgg tggcaagtct gaactgctgg
      721 attcagcact taaagatatt caggcagaaa tgacacccat cggcattcag gtaatgagcc
      781 tctcttatgt cggtaaaccg gaatacccgc caaccgtgat cgacagcatt aatgccaaag
      841 tcacagcgaa ccagaaaacc ctgcaacgcg aacaggaagt aaaacagcgc gaagcggaag
      901 ccaacatgtt gcgcgcggaa gcagccggac aggctgacgc cattcgcaca aaagcccagg
      961 ccgaagccga tgctattcgt ttacgcggtg aagctctgcg ccagaacccc ggtgttatgg
     1021 agctggaagc catcaataaa tggaacggca cactgccgca atacatgacc agcgatgcca
     1081 atacaccatt tatccaggtt aaataactta tatgcccggc aggccgccgg gctaagggaa
     1141 aagcagatga acacccataa tgcccaaccg caaataatga actatgaccc gaatctgacg
     1201 tcatgcggac gcatggcaaa acaaaccgtt cgattaactt tcgggttatg ggaataccgc
     1261 gaaacattcg aagttactgt cggcggcaat ctgaccggac tggatgttat cagttgcgct
     1321 attgaaagcc tgtacgcaac actgccttat gaagaagtcg aggatgagcg cacaggggga
     1381 acagatatca tggccaccat taatattggc gaactgatat gtcaggatga agacctgtcc
     1441 ggagagagct ggcttgctgg aatgcttgta gctgcagaaa ttatcagtat tgaacccgct
     1501 acaaatatac ggctctgaag ttctcgctat tcagagaaca ggagaaaaaa tgttcgattt
     1561 gattaatcaa ggacaactgt ataccgacag cgccggttac ccggtaaaaa ttattcactg
     1621 cataaataac atcgtgttgt acagaagaat ggatggacga acacagtcgg taaaaataaa
     1681 cgattttaat gaactgtttg aacgaatcga tcaccaggaa taccgacaaa ttctggcaga
     1741 aacagaacaa gaagctcatc tgaaaaaatt acgtgccatg aaaaggaagt aaagaatgaa
     1801 taaagcgttt gagctatggg tacgccagtg ttacggcaat cgctatgacc tgacgcgaga
     1861 tgttgacggt ttctactgcc gtgaagttgt gaaacgaatg tttgacgtgt ggtgccactg
     1921 ccgtggatga aaattttatg aggttggcat gcagacaatc atctatcaga taacccccag
     1981 caaatggtgt acggagagag tcctcattgc atcaacaggg ctaaagcctg gcaccattga
     2041 gcgggcaaga agaaagtcat ggatgcaggg aaaagaatac cgccattacg ctgtagaagg
     2101 tgatccaggg cattacagtg aatgcctgta caacatcgaa gaaattatgc gatggatcga
     2161 aaaccagaaa caaccaggtg tcaaaaatgc aagttccggt taacctgtta atgctcctgg
     2221 acgtctggga ggttcaatga gtaacacatc atacccgaca ggcgttgaaa accatggcgg
     2281 atcactccgc atatggtttc actataacgg caaacgtgtc agagaaaacc tcggtgttcc
     2341 tgacacagcc aaaaaccgga agattgcagg cgaactcagg acttcagttt gttttgcaat
     2401 cagaatgggg aatttcgact acgctgcgca gtttcctgag tccccaaatc tgaaacactt
     2461 tggtctggga aaaaaggaga taaccattaa ggcgctttca gaaaaatggc tggatctgaa
     2521 aaaaatcgaa ataggaggta acgcattcaa tcggtatcaa tctgtggtga gaaacatgtt
     2581 gcctcgtata ggggaaagcc gactggcttc atctgtaaca aaagaagatt tactcttcat
     2641 caggaaagat ttattgaccg ggtatcacaa tctctcaaac ggcaaaacaa cacctgttaa
     2701 ggggagatct gtagttacag ttaattacta catgacgaca attgcaggta tgtttcaatt
     2761 tgcagctgac aacggctaca cggaatcaaa cccgtttaac ggcctgacac cattaaaaag
     2821 gtccagaacc gagccagatc cgctcacgca tgacgaattc attcgtttta ttgatgcttg
     2881 ccaccatcaa caaacgaaaa acctgtggtc tttagcagta tacacaggca tccgtcacgg
     2941 tgagctaata tctctcgcct gggaggatat tgatttaaaa gctaaaacaa tgactatccg
     3001 tcgtaattat acaaaactcg gggagtttac tctaccaaaa accgaagcgg gaacagatcg
     3061 tgttattcat cttgttcaac cagccattga aaccctgaaa agtcaggctg aaatgacaag
     3121 atttggcact caataccaga ttgacgtcaa actgcgggag ttcggacgta ccgtaagcca
     3181 tgaatgcacg tttgtcttta atccgcaact ggtgaaaaag tgccagcaag ccggttacca
     3241 ctacaaagcg gactccatca gggattcatg ggcatcagcc ctgagacggg ccggactacg
     3301 gcacaggaaa gcctatcagt caaggcatac ttatgcctgc tgggcactgg ccgcaggagc
     3361 gaatccaagc tttatcgcaa accagatggg gcacgcaaac gctcagatgg tattcaatgt
     3421 ttacggagca tggatgaaag ataacaatat cgggcagata gaactgctca ataaggagtt
     3481 agctgaaagt gtcccataca tgccccacag agcaagacgc tgaaataatt tctttcagta
     3541 tcattgagtt aacccatggt tcccgccatg ttaactgcgt ggagggcaac accacgcttt
     3601 acgccctgcc gaaacccgag gttgtcctgc gctggcgtga gcagaccaca gatgacttcc
     3661 gcttctgttt taagtttccg gcgaccattt cgcatcaggc agcattacgg cattgcgatg
     3721 atttagtgac tgaatttttg acccgcatgt caccgttggc tccgcgcatt gggcaatact
     3781 ggctgcaact gcctgccaca ttcggcccac gggagctgcc tgcgctttgg cattttctcg
     3841 attctctccc tggcgaattt aattatggtg tggaagtccg ccatccacag tttttcgcca
     3901 aaggggaaga ggaacaaacg cttaatcgcg gtttacatca gcgcggcgtt aatcgggtga
     3961 ttttagacag ccgcccggtt catgcagcac gtccacacag tgaagctatt cgcgacgctc
     4021 aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac ggcgacaaat ccactgatcc
     4081 gttttatcgg tagtgatgat atgacgcaaa accgggaatt atttcaggtc tggttacaaa
     4141 aattagcgca gtggcatcag accactacgc cttatctttt tttacatacg ccagatattg
     4201 cccaggcccc ggaactggta cataccctgt gggaagactt acgtaaaacg cttccagaga
     4261 tcggagcagt tccggctatt ccacagcaat cttctctttt ctgaatttgc cacctatcat
     4321 agacaggtgc catcggccat tttaaaggga gtttgtatgg taagcgcgct gtatgccgtt
     4381 ttaagtgcgt tgttattaat gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac
     4441 cgcgttgcct atggtgacgg tggttttagc gaactgcaaa gcgctattcg cattcatggt
     4501 aacgcggtgg aatatattcc catcgcgatt gtgttgatgc tgtttatgga aatgaatggc
     4561 gcagaaacct ggatggtgca tatttgcggc atcgttttgc ttgctggtcg tctgatgcat
     4621 tattacggtt ttcatcaccg tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg
     4681 tgtgcgctgt tgctgatggt gctggcgaat ctttggtata tgccctggga gttggttttc
     4741 tccctgcgtt agcgcacaat acgccacttt ctttttcccg gatttttacg ttatgtctca
     4801 ccgcgacacg ctattttctg cccctatcgc cagactgggc gactggacct ttgatgaacg
     4861 ggtagctgaa gtcttcccgg atatgatcca gcgttccgtt cccggctatt ccaatattat
     4921 ttccatgatt ggtatgttag ccgagcgctt cgttcaacct ggtacgcagg tttacgatct
     4981 gggttgttct ctgggcgcgg cgacgctctc ggtgcgtcgc aacattcatc atgataattg
     5041 caaaattatt gccattgaca actccccggc gatgattgaa cgctgccgtc gtcatattga
     5101 cgcctataaa gcccctacgc cagtagacgt cattgaaggt gatattcgcg atatcgccat
     5161 tgaaaacgca tcgatggtgg tgctgaattt taccctgcaa ttcctggaac cttccgagcg
     5221 ccaggcgtta ctggataaaa tttatcaagg gctgaacccg ggcggcgcgc tggtgctttc
     5281 ggaaaaattc agtttcgaag atgccaaagt tggtgaactg ctgttcaaca tgcaccacga
     5341 ctttaaacgt gccaacggtt acagcgaact ggagatcagc cagaagcgca gcatgctgga
     5401 aaacgtgatg ctgaccgatt ccgtggaaac ccataaagca cgcctgcata aagccggttt
     5461 tgagcatagc gagctgtggt tccagtgctt taactttggt tcactggtgg cattaaaagc
     5521 agaggacgct gcatgatcga ctttggtaac ttttattctc tgattgccaa aaatcatctt
     5581 tcacactggc tcgaaacgct gcccgcgcag attgctaact ggcagcgcga gcagcagcac
     5641 gggttgttta agcagtggtc aaatgcggtg gagtttctac ctgaaattaa accgtatcgt
     5701 cttgatttat tgcatagcgt tacggcagaa agcgaagagc cactgagcgc agggcaaatt
     5761 aaacgcattg aaacgctgat gcgcaacctg atgccgtggc gcaaagggcc gttctcactg
     5821 tatggcgtca acatcgatac cgaatggcgt tccgactgga aatgggatcg tgttctgccc
     5881 catctttctg atttaaccgg acgcaccatt ctggatgtcg gctgcggcag cggttatcac
     5941 atgtggcgca tgattggcgc aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg
     6001 tttctgtgcc agtttgaagc ggtgcgtaaa ctactgggta atgatcagcg cgcgcatttg
     6061 ttaccgttag gtattgaaca acttccggca ctgaaagcct ttgataccgt cttttcgatg
     6121 ggcgtgcttt atcatcgtcg ttcaccgctg gagcatctct ggcagttgaa agatcaactg
     6181 gtcaatgaag gtgaactggt gcttgaaacg ctggttattg atggcgatga gaacacggtg
     6241 ctggtgccgg gcgatcgtta cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg
     6301 gcgctgaaaa actggctgaa gaagtgtggt tttgtagata tccgcattgc ggatgtgagc
     6361 gttaccacta cagaagagca gcgacgcact gaatggatgg ttaccgagtc tctgtccgat
     6421 ttcctcgacc cacatgatcc gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg
     6481 gtgctgattg cgcgcaagcc gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc
     6541 ttatccagcc tacaaaacct tgctaattca atatattgca gggactatgt aggcctgata
     6601 agcatagcgc atcaggcagc tttatgtttg cataacctca gcgcccgttt ccgggcgctt
     6661 ttcacgtctt acgcctgaac agctggctga tcaaacgccg ttaacttcgg tgcgttaccc
     6721 gtatattttt cgatatacac cagcgcagag ttaccggcac aggcatttgc cagctgcgag
     6781 ctggggatat ccgccgttaa cacgttcgca ctgccgtttt tacacaagcc attttccaga
     6841 tctggccatg caccttcatg cacgcatacc acgccttttt tgatcccgtc agtcaccacc
     6901 gcacctgtca gaatctgacc acgtttgttc cagacacgca ccagatcatc attcgcaata
     6961 ccaaagcgag cagcatcttc ggtgtgaata gtaattggtt cacgatcggc aaccgcatat
     7021 tttttacgca gttccgcata gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc
     7081 agaagctgca actgcttctc gtcggcggta cccttccact catcaggcgc aagccaggtc
     7141 gggtgtgccg ggcaatcctt atagccaaat ttctccagcg tttttgagta gatttcaatt
     7201 ttgccacttg gtgtacccag cgcatttttc accggatctg cgcggaaatc gccatagcga
     7261 acgaactgct cattcttctc gctgcggcgc atttcaagca atttattttg ctgccagaag
     7321 gcattaaaca tcggcatggt gacgcgttgc gcacgcgcac ctttctgagc ggcatcatag
     7381 aaaaatttca gccacgccat ttcatctcta ccttcggtat agatctcttt tccgcctggt
     7441 ttcagtaatt ccgcaaggtc ggcaaacacg tcaaaatcgt tgcgtgcttc aaattgcgga
     7501 gcgacaacct gcttcatcgg cacaatatgt tggttactgt aatcgccggt catcgtcaga
     7561 tcattgcctc gaacgatgtg gtgatcggta atacgatatc cgcgtgttta gctgccgccg
     7621 tccagtagca ctcagaaacg acgatcatct ccggtttctg ccaggcttta atcagacgat
     7681 tagtatcctg gtgatgggta aagttaccgc cacccgccca ccagatcatt ttgatattcg
     7741 gatacgtttg ttctttaccg ttatgctgat atttgccgcc aggattttcc agcgcatcga
     7801 cgatacgggc aacaggaatc gccgtcattc cgccatcatc agcggcctcg ctggcctgtc
     7861 cggcaatcgc cgcagacatt tccggcagca cgccgccaac acgcgtcggg ttgccaccgt
     7921 tggagtaatg ataagagaaa ccaaaaccgc cgcccggtgt accaatctgc cctaacatcg
     7981 ccgccagcgt caccagcatc cagtgtttct gttcaccata ttgctggcgc tgaattcccc
     8041 agcctgccat cagcattgtg cgattcgccg ccatcagttc agcgagtttg actatctgcg
     8101 cttccggtac gccagtaatt tccgccgccc aggccgcgct cttcggagta ttatcgctct
     8161 tacccgtcag atactcttca aactgcggat acccggtggt gtatttttca aggaacactt
     8221 tatcgtgttt gccttgtgtc atcagggtat gcgcaatccc taacatcagt gccacgtcgg
     8281 tgcccatatt cggcgcgatc caggtggcat tatcgccaaa gaactcgatg gtttcggagc
     8341 ggataggatc aatggcaatc actggtttgc cagatttttt cagctgatgg aagtattcca
     8401 gcccttgctc atcggtactg ctccaggcaa tttttaaggt attcagcggg ttcattcccc
     8461 acagcaccac aacctggctg ttttccagaa tcagcggcca ggaggtctgc tgttcataca
     8521 cctctacaga accgaccaca tgcggcatga tcacctgtgc tgccccggtt gaataatcgc
     8581 cgctatgccc ggaataaccg cccgccaggt tcatataacg ttgcagtaag gtttgcgctt
     8641 tatgcaacac gccagaagag cgccagccgt aagatccggc aaaaatggcc gatggtccgt
     8701 tagctttacg aatacgctca tgttgctcat gaatcagttt taatgcttgc tcccagctca
     8761 cctgtacata ggtatcttcg cctcgacctt tcgccggttg cagtggatta tcgagatagc
     8821 tttttctcac catcggatgc tgaatacgcg ccgtggtgtg tacctgatcc gccgccgtag
     8881 actgtaagga gttcggtatg gttttcgcca acgcgcctgt cgaagaaaca atcttgccgt
     8941 ccttcacttc tacgttcatc gctccccaac gtcccgcggt gaggatttta ccgcccttct
     9001 cttctgccca tgcgggtagc ggtgctgccg atgtcaccac cagcgctcca gcggcaatac
     9061 cgctgtgttt aataaattca cgtcttgtta atgtcataac ttcctccctg atcaacgagg
     9121 atcactgttt ctcggtaata tctttggcgt tgtactggaa ataccgcgtt aaaatgtcca
     9181 gttcgttttc gctcatgctg gttcgtgccc ccattccttt ggcaatggaa ggccacgcat
     9241 tgacggtgta atggtcggcg gcaatagggg catgacaacc agcgcaatag gtatcgtcaa
     9301 gtttttcagc gtattgccat agcggtttac ggtccgctaa tgcgggatcg gtaagcgcac
     9361 cctgtaaaga cgcctgacgc cattgattgc cgtattcgtc agcctgccat tccccgttta
     9421 ccgtgagcgc cttgatacct tcttcactta atgtggctag cgccagccgt tgacctaccg
     9481 ccaggtagag cgtgttttca ctgccctgca tttgataacc ctgcaacaga acgatcggct
     9541 gtttgccact ggcatcaacg acggtgagat cggtaccagg attcacggta gccagctcgc
     9601 ctatgcgaga agttttgaaa ggataaatat gtgcgccatt agtcactgaa gtagcggcct
     9661 gactttccag ctcatgcgcc gcgttgtcat ccatttttat ttctggcgga aaatgggcaa
     9721 tgcctttatg acaatcgata caggtttcgc cgcccttttg tgctttgtta tgcattttct
     9781 gcgcagattc actttgcgag gcaatatcca tggcatcaaa agaatggcaa ctacggcacg
     9841 ttgcagagtc agttgctttt aattctttcc atactgtttc ggccatttcc tggcgatgag
     9901 cttcgaactt atcgtcactg tctattttgc cgctaacaaa ttcatgataa atatctttag
     9961 atgcttttaa tttagcgaat aaataatcca tccctgactt tggaatatgg caatcggcac
    10021 attccgcacg tatccctttc tggttcgaaa agtggacagt tccctgatat tcctcaaaag
    10081 gtttactcat cgagtggcag gaaaggcaaa atgctgtatc cgacgtttta tgtaaggctt
    10141 tttgcgccag caagaacccg ccgccaccaa ccacaaccgc tatcagcaaa aacaataacc
    10201 caatgcgttt tttccctcgc atagattcct ctaattaaat tccagaacac cgaaatcatt
    10261 gtataaataa atacacaacg aattgactac ttgcttaatt cggcacaaca ctgcctgaaa
    10321 taatcgctaa agaatatgat ttattacaat gtaatcatta attgctaagg aataatccag
    10381 ttgttattta gaatatatat ttgtcagttt tagcaattta aatcacataa ttaatgtgaa
    10441 tatataaaat atttttagca caacgctatg tgcactgtca cccgctcaac agatcgcggt
    10501 aatcgttcat tttttatact attcagggat aaaaaaaggc ccctgttgaa attgcagggg
    10561 cctggtacga gcaagcatca tattgggcga catgatgcaa cggtaaaaat catttggcct
    10621 gatggcgttc aatgattcct ttcatttcag caaccgccgc cccgtctacg acataacgcg
    10681 aatactcgtc cgcgtgtgca tctgatgaca tggacaatcc ttgattacga taacgcatcg
    10741 gtgaggcttg ccacgctccc gcggagctat ggacttccag cactccggca tcgaggaagt
    10801 ggtgcaggtt ttctgcacgg actcctgctc cggccataat gattggagca tcacgatggg
    10861 caataagttc cataattttt gataaacctt gcagtgcgtc tgatttttgc cctgatgtca
    10921 gcacccgggc aatgcccaat tccgcaagat tattgagtgt atttagaggg ttagcgcaca
    10981 tatcaaaggc gcgatgaaaa gtcactgcca gcggaccggc agcagccatt attttttcca
    11041 ttcgtggcat atcgacattc ccgtcaacat cgagaacgcc cgtcaccagt ccaggaaaac
    11101 ctaattcgcg gaccgtgcgc acatcctcaa gaatggcggc aaactcaccg tcgctgtagc
    11161 aaaaatcacc accgcgtggg cgaattatcg gatgcacagg gatcgtcacc cgctggcgca
    11221 cggatttcag tacacccaac gacggcgtta agcccccctc ttttggggct gcgcataatt
    11281 caactctgtc tgcgccgttt tgctgcgccg ttagtgcaca ttccatgctg taacagcaaa
    11341 tttccagtaa tgccattttt actccttaat tacgccgact gctcgctggc aacgatctct
    11401 tcaatggacc acggatgaaa cttaatagtt gttttgccat cggtgaccgc cagcgtcgga
    11461 ttcggtaatc gctcatgctc accttttggc gaactggttt tcactgagat ccccggtagg
    11521 ctgagtcctt catcagaaag caatgccagc gcacgggcat tcagcgtttc tggatcaccc
    11581 ggcaaaacga tttcaatatg ttcccaacct tcgtgtgggt aacgtttttc cccgggccac
    11641 ggtagctcca caatagaaaa ctgccagtgc gcaacctgta ccggttcatg caatttaaac
    11701 agacaaatcg gtctgccatt gatcatattt tctgacaaaa gctcgccgca ctgttcaaac
    11761 ccgcgacgcc agcgttcagc ggtggcgttt tgatggcaac gcaaggaaat gtgatcggca
    11821 gtcaacggag tgatatccaa ccccaaacgg cgggaaagtt catctaatgc gtgggtaaat
    11881 cgcggtaaat ccgatgcaat atcctgcagc tcgtcgatag attgccagtt cgccataatc
    11941 actcttcgtc tttcagtaaa agcgttaatt taccctgttg ccctgcgcca accaaccgct
    12001 gatttcacgc cgcttctgat gcaatagtga aaacggcaat acgccacgcg cacgttgctg
    12061 acgaaaacag ccatttgcag tatactcccg ccctaatttc tttaactggt gcgggcaatt
    12121 tttgctcgct tcatcaatgt aaggtattcc ggtgaatatt caggctcttc tctcagaaaa
    12181 agtccgtcag gccatgattg cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca
    12241 gtcagcaaaa gttcagttcg gcgactatca ggctaacggc atgatggcag ttgctaaaaa
    12301 actgggtatg gcaccgcgac aattagcaga gcaggtgctg actcatctgg atcttaacgg
    12361 tatcgccagc aaagttgaga tcgccggtcc aggctttatc aacattttcc ttgatccggc
    12421 attcctggct gaacatgttc agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc
    12481 agaaaaacag accattgtgg ttgactactc tgcgccaaac gtggcgaaag agatgcacgt
    12541 cggtcacctg cgctctacca ttattggtga cgcagcagtg cgtactctgg agttcctcgg
    12601 tcacaaagtg attcgcgcaa accacgtcgg cgactggggc actcagttcg gtatgttgat
    12661 tgcatggctg gaaaagcagc agcaggaaaa cgccggtgaa atggagctgg ctgaccttga
    12721 aggtttctac cgcgatgcga aaaagcatta cgacgaagat gaagagtttg ccgagcgtgc
    12781 acgtaactac gtggtaaaac tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa
    12841 attggtcgac atcaccatga cgcagaacca gatcacttac gatcgtctca acgtgacgct
    12901 gacccgtgat gacgtgatgg gcgaaagcct ctacaaccca atgctgccag gaattgtggc
    12961 ggatctcaaa gccaaaggtc tggcagtaga aagcgaaggg gcgaccgtcg tattccttga
    13021 tgagtttaaa aacaaggaag gcgaaccgat gggcgtgatc attcagaaga aagatggcgg
    13081 ctatctctac accaccactg atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc
    13141 cgatcgcgtg ctgtattaca tcgactcccg tcagcatcaa cacctgatgc aggcatgggc
    13201 gatcgtccgt aaagcaggct atgtaccgga atccgtaccg ctggaacacc acatgttcgg
    13261 catgatgctg ggtaaagacg gcaaaccgtt caaaacccgc gcgggtggta cagtgaaact
    13321 ggccgatctg ctggatgaag ccctggaacg tgcacgccgt ctggtggcag aaaagaaccc
    13381 ggatatgcca gccgacgagc tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa
    13441 atatgcggat ctctccaaaa accgcaccac ggactacatc ttcgactggg acaacatgct
    13501 ggcgtttgag ggtaataccg cgccatacat gcagtatgca tacacgcgtg tattgtccgt
    13561 gttccgtaaa gcagaaattg acgaagagca actggctaca gctccggtta tcatccgtga
    13621 agatcgtgaa gcgcaactgg cagctcgcct gctgcagttt gaagaaaccc tcaccgtggt
    13681 tgcccgtgaa ggcacgccgc atgtaatgtg tgcttacctg tacgatctgg ccggtctgtt
    13741 ctctggcttc tacgagcact gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag
    13801 ccgtctaaaa ctggcacaac tgacggcgaa gacgctgaag ctgggtctgg atacgctggg
    13861 tattgagact gtagagcgta tgtaatcgat ttttcgtgag agtgaagcct gatcagggtt
    13921 agccgatcag gcttttttat tgccatctaa atgtattctg aaaacggaca tgccatcgtt
    13981 ttctcactgt tgagtaagag gccagaagcg taatatccag gcccaggaaa acgataacgg
    14041 ttcaatttaa ggaataccgc agtgtttaaa tttcttgtat taacattagg cattatctct
    14101 tgccaggctt acgcagaaga tacggttata gtaaatgatc atgacatttc agccatcaaa
    14161 gattgttggc aaaaaaattc agatgatgat actgacatta acgtgatcaa atcatgtctg
    14221 cgacaagaat acaatctcgt cgatgcgcaa ttaaataaag cttatggtga agcttatcgt
    14281 tatatagaac aagtgccacg cacgggtgca aaaaaacctg ataccgaaca acttaacttg
    14341 cttaaaaaat cacaacgagc ctggctggat tttagggaca aagaatgtga attaatcctt
    14401 tcaaatgagg atgttcagga tttaagtgac ccttattctg aatcagaatg gctatcatgt
    14461 atgatcatac agaccaatac gcgaactcgc cagttgcagc tataccgtaa ctctgaagat
    14521 ttttatccaa gccctttgac aagaggataa ttcacatctt tttggcatgt tttgttgcaa
    14581 gctattcctg ataaataatt gcaacaaaac atcgagcctt tttcgctgag ttattaaaca
    14641 tgctcgcgag cgcgtaattt ttatttgtcc ttcagcgata attcacaatc acttcattcc
    14701 gttgtacctt tagcggtgga attaaccgcc caccgccagg cacttcccag ataaagcgca
    14761 acggttctgc tgccgctata ccggaaaagg ccacggtggt tccgctctgc ccctctaatt
    14821 cgacacaacg agattgcgaa cacaagcgaa cccgcagtcc tgcaggtgtc gggccgataa
    14881 gctgataccg ccacgccacc agcgtcatca atcctgaagc aggttgtcga gtagaaagag
    14941 gcgctgacga catcgactca ccgcgatgat ttagcgtaat tccgacacta cttgcctgcc
    15001 acatcccctc cccggcggct tgcaccagca gcggaaacaa taatattgct aataaggttc
    15061 tcatcattta ccgccaattg tcgccgtcat gcggatatgt cggttatcag atagttccag
    15121 attcgacagc accactaact gcggcaagct gcggcgcaga aagcgagaca ataatggtcg
    15181 cagcgcgtgg ttcaccaaca atactggcgg cgcccccaac atctcctgac gggatagcgc
    15241 ttcctgagtt tgcgccagta aacgatccgc cagccctggc tccagtccgc ccccgccctg
    15301 caacgcctgt agcaacaaac gttccagcgg tgtatcgagg ccaataacat ggacttcatc
    15361 tttgccagga aaccactgct gggtaatcgc ccgtcccaac gccacgcgca cgacggcggt
    15421 taattcatgt ggatcacttt ggatgggtgc atgttccgcc agcgtttcga gaatagtgcg
    15481 catatcgcga atcggcactt tttcatcgag cagattttgc agcactttat gcagtgtggt
    15541 gagcgtgacg acgccaggaa cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg
    15601 atccagcagc tgttgcgcct cctgacgacc aaacagctct gcggcatgct ggctaatgag
    15661 gtggttaaga tgcgttgcta ccaccgtgct ggcctcaacc actgtgtacc cctgaatctg
    15721 cgcctgttct tttagcgcac tttcaatcca gatagcattc aggccaaatg ccggatcgac
    15781 ggtcgcctca ccaggtaacg tcccggcagc ggtaccaggg ttaattgcca gccagcgccc
    15841 cggataagca tcaccactgc caatctctac gcctttcatc aaaatacgat aacgtgccgg
    15901 ttgcagatcc atattgtcgc gaatgtgcac caccggcggc aagaagccca tttcctgagc
    15961 aaatttctta cgaatactac gaatacggcc cagcaactcg ccatcttgtt ggaaatcaac
    16021 catcgggatc agtcgataac ccacttccat tcccagagaa tcttccagtt gtacatcgtt
    16081 ccacgtcgct tcgacaacgg tattattctc tgccattttt accggtttgg gttcggcagg
    16141 cgctttttgt tcgcgtccgc gtatccacca ggccagcccg agcaatccgg cagtgaacag
    16201 caaaaatacc aggttcggca ttccaggcac caggccgagt aaaccgagca cggcggcgct
    16261 taacaacata acgcttgggt tactgaaaag ctgattcacc atctgctcgc caacatcctg
    16321 atcggtgctg acacgcgtaa cgatgacccc cgcggcggta gaaatcacca gcgccggaat
    16381 ttgtgccacc agaccgtcgc caatggtcaa tagcgtataa ctttccgccg cgtgtcccat
    16441 gctcatgcca tgttgcagca cgccgaccag caacccgccg acaacgttaa tgaccatgat
    16501 gaggatcccg gcgatggcat cgccgcgaac aaacttactt gccccgtcca ttgagccgta
    16561 aaaatcggct tcctgagtca cttcggagcg gcgttttttc gcctcatctt caccaatcaa
    16621 tccggcgtta aggtcggcgt caatcgccat ctgcttaccc ggcataccat cgagaacaaa
    16681 gcgcgcaccc acttctgcga tacgtccggc acctttggta atgaccataa agttgatgat
    16741 cacgagaatg acgaacacca cgataccgat agcgaaattg ccaccaacga ggaagtgacc
    16801 gaacgcttcg accaccttcc cagccgccgc cgcgccggta tgcccttcca tcaaaatgat
    16861 acgggttgaa gccacgttaa gtgccagacg caacagcgtg gtaaacaaca gaatggtcgg
    16921 aaacgcagca aactcaagcg tgcgctgggt aaacatcgcc accagcaaca ccatgatcga
    16981 caaggcaata ttgaaggtaa acagcaggtc gagtatgaat gcgggcagtg gcagcaccat
    17041 catcgacaag atcaacagga tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt
    17101 caggtttgtg ggcaggcgca gcatcgcggc cagattactc atgggtcggt ttctcgttaa
    17161 taaaatccag ggcttccggc accggaagat gagtaggttg tacagggcgc tgtccaccag
    17221 ccagacgcca gcgtttcagt tgccagaccc aggccagcac ttccgccacc gcggcgtaca
    17281 gttgccccgg gatttgttga ccaatctccg catgtcgata cagcgctcgc gccagcggcg
    17341 gcgcttcaag cgtcgggacg ttattttcag cgccgatttc acgaatgcgc agcgcgacaa
    17401 gccctgcacc tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaacg
    17461 ctaccgaata gtgggtcggg ttattgacaa tgacatccgc tttcggcaca tcggccatca
    17521 tccgacgccg tgcggcagct cgctgcatct gacggatccg tcctttaaca tgggggtcgc
    17581 cttcgctttg tttgaactca tcacgaatat cctgccgcga cattcgcagc tttttcaggt
    17641 ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca
    17701 gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc ggagactcgg
    17761 ccattaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa
    17821 ccaggatggt tttcaaaatt gctttaagca actccgcgcc agtctgtgcc gagaacatcc
    17881 gttttatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat tttccgctaa
    17941 ataccagccc ccccagcata accggagaaa tgagcgccac cagcaccacg ccgctaatca
    18001 gcggtagcag cgccagcatg gcttctctga tcagcagaat aatttgcccg aggatcagat
    18061 tcgggtcatt gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc
    18121 gacgggccag cgacacaccg ccaaaccaga taacactaac gcccaccagc aaaatcagca
    18181 gtgaggtcag ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt
    18241 gaggtgtggg ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa
    18301 gagtattaag cgtaaatgat gccagagcgc aaagcgttca atggtttgag taaggggcaa
    18361 aacaggcggg atttagggct tttgctgcca cacatcaagc atagtgtgcg tttgtcggat
    18421 gcggcgtcat cgccttatca gaccgcctga tatgacgtgg tcacgccaca tcaggcaata
    18481 caaatcaaaa tccaagacta tccaacaaat cgtccacctg atcctgactg gctaccacac
    18541 cggctttgct ggtatcgacc tgaggtccat taagcaaact ctggttttca cgttttggac
    18601 gcgactcctg ttccgggatg ttttccaaca gcaccatcag caactggcgt tcgatctcct
    18661 gaatgacatc catcatccgc ttaatgacct gcccggtgag atcctgaaaa tcctgcgcca
    18721 tcatgatttc cagcagttgc gcgttagtaa agctggtatg cgcgggtaca tctgccagaa
    18781 atttccgcgt atctgttacc agttcacggg cgtcggcaag gtcaatcgga tcggcaaacc
    18841 agtcatccca acgttgggtt aacgcttttg ctgatttctc catttgatcc tgatgcggtt
    18901 gtgacgcctc aacactgttc agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat
    18961 agtacaaacg atcgcgcgca tccgggatgg cttccgccgc ttcggcaatg gcctgatcca
    19021 gccccagttc ccgcaaactg tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga
    19081 tatcgccagc tgaatgctcg tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct
    19141 cacatgccca gtttctcaaa gattttgttg agtttttcct ccagcgtcgc ggcggtaaat
    19201 ggcttcacca catagccact ggcccccgct tgcgccgcag caatgatgtt ctctttcttc
    19261 gcttctgcag tcaccattaa cactggcaat gctgacatcg cgccatccgc acgaattgtt
    19321 ttcagcaatt ccaggccatc catgttgggc atgttccagt cggaaataac aaatccataa
    19381 ccgcctgcct gcaacttatt gagagcgtcg acgccatctt ccgcttcctc aacattattg
    19441 aatcccagct ctttcagcag gttacgcact atgcgtcgca tggtggaaaa gtcatccaca
    19501 accaaaaatt taagttcttt atccgccatt tcacactcct gatttaaata cgtatcgcct
    19561 gtccggcact aatttttgcc agcatttgct ggcttacctg gctaagatcg accacttcgc
    19621 agacaccacc catattgatg gcctcacgcg gcatgccgaa caccacgcaa cttgcttcgt
    19681 tttgcgcaag ggtccatgcc cccgcctgac gcatcgccaa cattcctgcc gcgccgtcgt
    19741 tgcccatacc ggtcaggatc accccaaccg cattacgccc cgcctgtttg gcgacagaat
    19801 ggaacaacac atctaccgaa ggccgatgac ggttaaccgc cgggccatcg tgaattttga
    19861 tttgataatt tgcgccacta cgcgccagct ccatatgccg atcgcccggc gcgatatagg
    19921 catgccccgg caagacacgt tctccgtctt cggcttcttt aaccccgatc tggcaaagct
    19981 tattaagtct gtcggcaaaa gagcgggtga aaccgggcgg catatgctgg gtaattaaca
    20041 gtgccgggct ggaaagcggc aacggttgca gtacgtgacg aattgcctca gttccacccg
    20101 ttgaagcacc aatcgcaatc agtttttcag aactcaacaa cggccctgcc ttcagcgttg
    20161 tcggtgccga caatggctta tgtgctgcaa ggctcgcctt tgctgctgta cgcacctttt
    20221 cagcaatcat ttcgctgtac gccagcattc cttcgcgaat acccagttgc ggtttggtga
    20281 caaaatctat tgcccccagc tccagcgcgc gcagcgtgac ttctgatcct ttgccggtca
    20341 gggaagaaac catcacaacg ggcattggac gcaaacgcat taatttttcg aggaaatcca
    20401 gtccgtccat ccgcggcatt tcaacatcca gcgtcagcac atcgggattg aatttcttaa
    20461 tcaagtcacg cgcgaccagc ggatcaggcg cggtcgccac catttccatg tcgctatggc
    20521 tgttgatgat ttctgtcatg atctggcgca tcagtgccga atcatcgaca gataacaccc
    20581 tgattttgct catcgttaat ccttacttag cgcatacacc gtctgaccac gcagcgtgaa
    20641 gcggcgctca aggtggctaa agttttcaga gtgacccgca aacagcaatc cgtcgggttt
    20701 aaggagcgga acaaagcggc gcaaaatctc ctgctgggta gtttgatcga agtagatcat
    20761 gacgttacga cagaagatcg catcaaacgg ccccggcacg gtgtactgtt tcgccagtag
    20821 attcagcggg gcaaaatcaa catagttcgc cagctcctga cgcacgcgta ccagcccttc
    20881 atgcggtccc gtccctcgca tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa
    20941 ctcttcatgg cgatagatac cgcttctggc tttttccagc acttcggtgt cgatatcact
    21001 ggcaaacact ttccagcgtc cgggcgcggt gcccaatgtg tcagccagcg tcatcgcaat
    21061 gctgtacggc tcttcgccgg tcgaagccgc cgcgctccat acgcgatact cgccagaacg
    21121 gcgacgtgcg tgatccgcga gcagagggaa atgatgtgcc tcacggaaaa atgccgtcag
    21181 attcgtggtc agcgaattga taaacgcctg ccactcacgg ctgtgctgat tagattccag
    21241 caagttcaag taatgaccga aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg
    21301 gttgtaaacc atgtcgcgtt tatggtcagc cagaacgatc ccggctcgtt gatagatcaa
    21361 ttgacttatc cgccgaaaat gtgcgtcgga aagcgccagg cgctcggtca tctgtaacaa
    21421 taaagacgtt tgcccacagg gcagagatga agtcatagcg ccttctcaat cacttcagga
    21481 taccactggc gcaatttgta actgcgccga ctcatgtcgt gctacttcat gttcttcaag
    21541 ggtaaatacc gccacacgcg acgaaagatg gtcggcctga ttcgccagtt gttccgttgc
    21601 caccgccgct tcttctacca gcgaggcgtt ctgctgagtc acctgatcca tctggctgac
    21661 agcctgtgca acctgctcaa tcccccgccg ttgttcttcc gacgccgaag caatttctcc
    21721 cataatgtcg ttcacgcggg tcaccgaact gacaatatcg gtcatggtcg cggcggcgtt
    21781 attcaccagt ttcgaaccct gctggacacg attgactgac tcttcgatca gccctttgat
    21841 ctcttttgct gcctgcgcgc tacggctggc aagattgcgc acttcacctg ccactaccgc
    21901 aaaaccacgc ccctgctctc cggcgcgagc cgcttccact gccgcattca gggccagaat
    21961 attggtctgg aaagcaattc cgtcgataac gctgataatg tcgccaattt tttgcgagct
    22021 ggtggcgatc tcctgcatgg tgtgagtcat ggtactgacc tgaacaccgc ccgcctgcgc
    22081 cgttgtcgca gcattttttg ccagttccga cgcctgtcgt gcgttatcgg cgttttgccc
    22141 taccgtggcg gttaattgct ccatactggc agccgtttgt gccagcgatg ccgcctgctg
    22201 ttcggtacga cttgagagat cgttattgcc tgcgacaatc tctgcgatcc caatgtgcat
    22261 ctcatggctt cccttacgca catcactcac cgtcccacgc aaagcctgct gcatggtctt
    22321 caggctggca aaaatggcgg tgatctcatt acgaccatat accgcaatcg gacgcgccag
    22381 attacccgca gcaatgctgt caaaatggct accgataatg gccagtggtt gaacaatcat
    22441 cttgcgcgtc caccacagcg cactgctgat gtagatcgcc gcaacaataa tcatgctgat
    22501 aaacaccagc gccgaaatct gatagttacg ctggctttgc gcactggcgg cttccagcac
    22561 atggttgatc tccacctgcc aggcctcaaa gttaacgtca aacgcattct gtgatccctg
    22621 caccggcgca gtgaggaaat ccgaaagttg attactttca agccaggtcg cctgatgttc
    22681 gagatcgttg tgccagcggg caaaactttt ctccgtctct ttttgcaatg ccctgacgtg
    22741 ctcgttgccc gcagtcatcg ccataaaact tttaaacagc gtggtggatt gcgtcagact
    22801 ggcgcgcgcc gtcgtcatca acgttttaat gtcatccgcc ggatagctaa gtgccgtcag
    22861 agtgcccgct ttgttcagcg cggtactggc ctgtaacatt accgcccgag tttgcgctaa
    22921 tgccgcacgt tgctgattgc tctgctcgac ctgattcagc cgttgcaaat cgtcgcgaaa
    22981 tgcccaaaaa gacatgccgt tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa
    23041 aaacagcgtg gtcgaaattc gaatacgatt aaacatcaac gctccccatc aggcggcaat
    23101 gaccgcgtta gtaaatactc gtcaaaatgt ttcccagttt ggatcttgtt cagcaattcg
    23161 cagtcgtggc tgtgccggtg attgctcact ggcaggacgg gatggtgttt gcggtttatt
    23221 ggtgagtggg ctggctgcca gacgaaacgc ggaaaccgct tgcgttaaac gactagcctg
    23281 ttcttccagc gcagctgcag cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt
    23341 gacgcgatcc atttccgaaa ccgccaatgc gacttgatcg atgccacggc tctgttcatc
    23401 cgatgccgat gcaatctcgc ccataatgtc agtcacgcga gtgacagcat tgacgatatt
    23461 gttcattgtt tccccggcgc tttcgaccag caccgaaccg gtatcaacgc gcgagacgga
    23521 gtcttcaatg agagctttga tctcttttgc cgcctgggcg ctacgactgg caagattacg
    23581 cacttcgccc gccaccacgg caaaaccacg gccctgttca cccgctcgcg cggcttcaac
    23641 cgcggcattc agcgcgagga tattagtctg gaaggcaata ccgtcgatta cgctgataat
    23701 gtcggcgatt ttcttcgaac tatcggcgat ctcatgcatc gttttcacta cgccatccac
    23761 cactttgccg ccgtgctggg cggtgtcgga ggcactttgc gccagttgcg aggcctggcg
    23821 ggcgttatcg gcgttttgct tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc
    23881 ttccagcgcg gatgcctgct gttccgtacg ggaggaaaga tcggtgttac ccgccgcaat
    23941 ttcacgggta ccggcataga tggcatctga accttcgcgg acatgagtga cggtgtcagt
    24001 caaagagcgt tgcatatgtg aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc
    24061 aatggtcagg gtattcgcca ggttaccacc ggcgatttcg cgaatgtgag caataatttt
    24121 tgccagcggt gtaagcaaca tacggcgaat gccataccac gccaccagca gaatcaatac
    24181 caccaccagc gcgataaccg ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc
    24241 agtgacgata tcgcgataca gtttttcact gctgagggcg tactgagcaa acgcttcgcc
    24301 cattgcattt tgcattccct gggttggctg agcgaaataa gctccagtat tgccataatc
    24361 aagataatca atcagttcag ttaacgctgt gtaatagttt ttatattttt catcaatatt
    24421 acgactggta gcgaccattt caggtaacgg tgccatgctt ttgaattttt tataatgagt
    24481 cgcggcctgg gccaatgttt tcctggcgct atcgagcaat tcaactttgg cgttactttg
    24541 ttgattggag gaatccatca tcatccgcac tgctgaacga ctcaggttaa tgcgcgtttg
    24601 cagcattaaa tcccaggttg acgtcagctc gccctgctgt tcccgtaatt gattggaaac
    24661 cacaaaactc ttctggctat ggtgaaggga agaaaaaaac agactgccgg aaataagctg
    24721 taacagtgcg aataccccca gcaccattac caacagcgtg actacgcgga tacggttaat
    24781 cataaggcac cttcctgaaa acaagttgat ctcgttatcg gcaaggaagg gggaaacttt
    24841 attgctgatg ccacccgccg cgaaattgaa ataaaacccg atgcgcagat catcgggttc
    24901 atttcaattg aggaaatcag gagaattacg ccacttctga cgccgcgcta tctaacagcg
    24961 ccatctcttc gctgttcagc agtttttcga tgttcaccag aatcaacata cggtcgccca
    25021 gtgcgcccag tccagtgaga tattctgttg aaagcgtcac ggcaaactct ggtgccggac
    25081 gaatttgctc cgccgtcaat gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca
    25141 cccgctgtcc gagattcagg acgataacta ccgtgttgtc gttatagtcc acatccacct
    25201 ggctgaactt aattcgtaag tcaacaatcg gcacaataac accgcgcaga ttcgtgacgc
    25261 ctttgataaa cgccggcgtg ttcgcaatcc gtgttacctg atcgtagcca cggatctcct
    25321 gcactttcag gatatcaata ccgtactctt catcaccaag ggtaaatacc agaaattcct
    25381 ggcctgacgg ctcgctggcc agctttgtta cattcgtcat accggtcata ttgttacctt
    25441 tttactcatt caggcggcgg tgttcgccat acgttgttcg cggtttatcg cctgcaaggc
    25501 ggagacatca acaatcagtg ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc
    25561 ggggactttg cgatagttac tttcaaggtt tttaaccaca acctggtgtt gaccaattaa
    25621 ttgatccacc agcaaggcgt agcggcgacc gccactttgt aagatcacca caattccctg
    25681 ggtagcttcg gttttcgcgc ccgcgacgtt gaacactttc cacagttcga cgatgggcag
    25741 atattcaccc cgcacttcca gcacccgctc gccgccggcc agtggatgga gatcggcttc
    25801 acggggttgc agtgattcca taacagcatt cagcggcaaa atgaaaactt catccgcaac
    25861 gcgtacggac atgccgtcga ggatggccag cgtcagcggc agtaaaatgc ggatcgtcgt
    25921 gccagtaccc tgcttcgact ggatttcgac atgaccgccc atctcctgga tattacgttt
    25981 aacgacgtcc atgccgacgc cgcgcccgga gacgtcggtg acctgctctg ccgtggagaa
    26041 gcctggtgca aatatcagca tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa
    26101 accttgcgag gccgcttttg ccagaattcg ctcacggttt agccccgccc catcgtcggt
    26161 cacttcaatg caaatgttgc cgccctgatg ttcggcagac agaattaaat ttccgacgct
    26221 gtttttacct gcggcgagcc gtttttctgg cagttcaata ccgtgatcga ggctattgcg
    26281 taccaggtgg gtcagcgggt cgataatgcg ttctatcagg ctcttgtcga gctcggtgga
    26341 actgcccacc agcgtcagtt ctacctgctt gccgagtttt cccgccagat cacgcaccag
    26401 ccggggatag cgactaaaga catattccat cggcatcatg cgaatcgaca tcactgattc
    26461 ctgcaaatca cgggcgttac gttgtaactg ccccatgctg gtaatcaaat caccatgatt
    26521 aaccgggtcc agttcgctgg aacgctgggc aagcatggac tgggtgataa ccagctcgcc
    26581 gacgaggtta attaattgat caaccttttc taccgctaca cggatgctgg tggattcact
    26641 gctacgcgtc gttttttccc gctccacgcg accggttggc gcttgttcgg ctgccagttt
    26701 aagcactggt ggggtggata tttttggcga gacttctact gtttcaaagg taatctgatc
    26761 ggcttcaatc acaaaacaga gtaccgctgt gatgtcatct tcggcgatat cgcccggtaa
    26821 tattgccgag agagaatccg cccctttcac cacgtcagtt aacgttgtca gatgccccag
    26881 ctcttcttcc agcaggtcga cttcgccggc cttcaggcgc gaaaggataa ttcgtcgcgg
    26941 caactgactg cgactctgct catcttgcgg ttcacttttg gcaaccacac ttaatcgggt
    27001 cactgcggat ggcgtttcgc ctttcgcttc taatgccagt tgacgcaagg cctggcagat
    27061 ataatcgaag ctggcggcat ccggctcttg cgactgttta taagcgtcga gctgttcttg
    27121 catgatgtcc ttcgtttcca aaaacagatt gataatgtcg gtgttgagtt gcatctcacc
    27181 tcgtctggct tcatcgagca ggttttccat cagatgcgtg gtttcctgca aaacgccgaa
    27241 gccaaaagtt cctgcccctc ctttgatcga gtgggcagcc cgaaagatgg cattcaattg
    27301 ttcggcatct ggcgcttccg gctgcaaaac cagcagatgc tgctccatat cagccaacag
    27361 ttcgtccgct tcatcaaaaa atgtctgata aaaatcgctt atatccatgc tcacgctgtc
    27421 acctcggttc ggctgatggc attgtgggaa cactgacctg tggtgcaacc tcaggttttt
    27481 ccagggcgct tactggctca ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg
    27541 cttgtttgtt cagaaccagc aggctgatgc gacggttgac ggcatcatca ggtccgcgat
    27601 cgcttaagcg catcgttgcc gccatgccga cgacacgtaa cactttgccg ctatccaacc
    27661 cgccgaccat cagttcgcgg cgggatgcat tggcccgatc ggcagaaagc tcccagttgc
    27721 tgtaaccttt ctcaccgctg gcgtagggaa aatcatcggt atgacctgaa aggctaatac
    27781 ggttgggaat accgttcagt acaggcgcga tggcgcgcag aatgtcgcgc atatagggtt
    27841 cgacatcggc actgccggtt ttaaacatcg ggcgattctg gctatcgatg atctgaatac
    27901 gtagaccttc ctggaccaga tcgattttga gatgtggacg taacgcccgc agtttcggat
    27961 cggactctat caactggtcg agatcacccc gcaatttccg caatcgactt tgctccatgc
    28021 gttttttcaa ctcttcgatg ttcggctgct tattcacttc cccctggctt tgggtgtaat
    28081 catcaccacc gccggggatt gggctttcac tattagaaat gcgattgccg cccgtaaccg
    28141 ctgtcgccag tggagtccgg aagtactccg caatctgaat caactctttt gggctggaga
    28201 tggagatcag ccacatcacc agaaaaaagg ccatcatcgc agtcataaag tcggcataag
    28261 caatcttcca cgatccatgt gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa
    28321 taatcggatg cgcttgattc ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt
    28381 caccgcacgc acatgctctt ccagttcaat aaacgacgga cgttcgctgg aatagagcgt
    28441 tttgcgacca aactcaacgg cgataggcgg tgcgtaaccg ttcagattag aaagcagtgt
    28501 gactttgacg cactgcatca ttttgctggt ttcggcgctt ttctgacgta aaacactcgc
    28561 taatggggaa ataaatccgt aagccaataa aatgccgagg aaagtcccca ccatcgcatg
    28621 tgcgataagc gcccccagct cggcggcagg acgatcggct gaacctaacg cgtgaacgac
    28681 ccccattaca gccgcgacaa taccaaacgc cggaagtgag tccccgacca gcgccagact
    28741 gttcgccggg acttctgcct cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc
    28801 gatttcgaag gtgttcatgt gaccgctgat aatcaggcgc agataatcga cgataaaatc
    28861 aagcatgacg ctatccgcga ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg
    28921 attttcaata tcacgttcca gtgaaaacat ccccatctgc cgcgatttcg ccatcaaccg
    28981 ataaagcaga gccagcagat ccatatacat tgctttggtg tatttggagc gacgaaacag
    29041 caacggcagc gctttcagcg tgcctttaat cgctttgcca ttattgccga cgataaacga
    29101 cccaatccct gcaccggcaa taatcaccag ttcagcgggt tgatagagtg ctccaaggct
    29161 tccaccggtc atcaaataac cgccgaaaac tgtaccgaga acaaccaggt aacctaataa
    29221 gataagcacg acatcatcct tccactgttg accatgacag gatgttcagt cgtcaggcgt
    29281 taacgcgcga ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc
    29341 gccacatcgt atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat
    29401 atcggcagga ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gttgacataa
    29461 gctgcaggca aagctgccaa caggctggtg agcgtgggta ataaaattgc cgccgcagca
    29521 gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgcaccaatg tccaggcacg
    29581 ggttaatgcc agcagcggtc cttcttctgc ttgcgggcac tgttcaaggt ataaacggta
    29641 ggctttgatc accgcatcga cgccattaca caaaccggtt ttcagtaaaa actgccatgc
    29701 attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa
    29761 tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg
    29821 tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtgatcaa
    29881 ttccattgcc agctgaatat cccgcgcttc ctgaacaatg cttttttcac tcatgatcag
    29941 gcccttttct tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa gcgtgttgag
    30001 agcatgatgc cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg cgtcaactga
    30061 gtaatcgtct ggtggctgtc aaaacggaag tgacaaacca gctgattggt ttctgccagc
    30121 ttaaccattt gcggaagagt cagtgccgct aatgttgtcg ccatttcttc atttatgccg
    30181 agacgaaaca tagcggacgc tttgtcctga acaatcagac gctgtgcaag tagtaaatat
    30241 gacaagttga tgtcataaat gtgtttcagc aactcggagg tatgcattat tcccacccag
    30301 aataaccaac tttattttta tgcggtttca ccgcaccccg tgatgtcgcc gggaagcccc
    30361 ggtaaaaaat aattagcatt agaatagttg cgataagctg caataagcag agccaccttt
    30421 ttggtttaat aagtccttac aaatagaagg gggtctttac acttatctaa gatttttcct
    30481 aaatcgacgc aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt
    30541 caataatgtg tgatgcagat cacacaaaac actcaattac ttaacataaa tgcataagtc
    30601 atacttttgt t
//
LOCUS       DAZGBC010000046        30061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGBC010000046 DAZGBC010000000
VERSION     DAZGBC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.046"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..644)
                     /gene="fimA"
                     /locus_tag="ACHK1J_002041"
     CDS             complement(102..644)
                     /gene="fimA"
                     /locus_tag="ACHK1J_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7911312.1"
                     /translation="MKLRFISSALAAALFTATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            1123..1989
                     /gene="folD"
                     /locus_tag="ACHK1J_002042"
     CDS             1123..1989
                     /gene="folD"
                     /locus_tag="ACHK1J_002042"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO7911313.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1991..2203
                     /gene="ybcJ"
                     /locus_tag="ACHK1J_002043"
     CDS             1991..2203
                     /gene="ybcJ"
                     /locus_tag="ACHK1J_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO7911314.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            2311..2832
                     /gene="ybcI"
                     /locus_tag="ACHK1J_002044"
     CDS             2311..2832
                     /gene="ybcI"
                     /locus_tag="ACHK1J_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7911315.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2868..4253)
                     /gene="cysS"
                     /locus_tag="ACHK1J_002045"
     CDS             complement(2868..4253)
                     /gene="cysS"
                     /locus_tag="ACHK1J_002045"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO7911316.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            4427..4921
                     /gene="ppiB"
                     /locus_tag="ACHK1J_002046"
     CDS             4427..4921
                     /gene="ppiB"
                     /locus_tag="ACHK1J_002046"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO7911317.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4924..5646
                     /gene="lpxH"
                     /locus_tag="ACHK1J_002047"
     CDS             4924..5646
                     /gene="lpxH"
                     /locus_tag="ACHK1J_002047"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO7911318.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARNADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5764..6273
                     /gene="purE"
                     /locus_tag="ACHK1J_002048"
     CDS             5764..6273
                     /gene="purE"
                     /locus_tag="ACHK1J_002048"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO7911319.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            6270..7337
                     /gene="purK"
                     /locus_tag="ACHK1J_002049"
     CDS             6270..7337
                     /gene="purK"
                     /locus_tag="ACHK1J_002049"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO7911320.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(7476..8369)
                     /gene="ybcF"
                     /locus_tag="ACHK1J_002050"
     CDS             complement(7476..8369)
                     /gene="ybcF"
                     /locus_tag="ACHK1J_002050"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7911321.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(8366..9181)
                     /gene="ylbF"
                     /locus_tag="ACHK1J_002051"
     CDS             complement(8366..9181)
                     /gene="ylbF"
                     /locus_tag="ACHK1J_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7911322.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVTQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(9192..10451)
                     /gene="ylbE"
                     /locus_tag="ACHK1J_002052"
     CDS             complement(9192..10451)
                     /gene="ylbE"
                     /locus_tag="ACHK1J_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7911323.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(10461..12128)
                     /gene="fdrA"
                     /locus_tag="ACHK1J_002053"
     CDS             complement(10461..12128)
                     /gene="fdrA"
                     /locus_tag="ACHK1J_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7911324.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFRHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALCLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGNLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            12445..13494
                     /gene="allD"
                     /locus_tag="ACHK1J_002054"
     CDS             12445..13494
                     /gene="allD"
                     /locus_tag="ACHK1J_002054"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO7911325.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            13516..14751
                     /gene="allC"
                     /locus_tag="ACHK1J_002055"
     CDS             13516..14751
                     /gene="allC"
                     /locus_tag="ACHK1J_002055"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO7911326.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLETQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPHVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            14762..15547
                     /gene="allE"
                     /locus_tag="ACHK1J_002056"
     CDS             14762..15547
                     /gene="allE"
                     /locus_tag="ACHK1J_002056"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO7911327.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(15676..16821)
                     /gene="glxK"
                     /locus_tag="ACHK1J_002057"
     CDS             complement(15676..16821)
                     /gene="glxK"
                     /locus_tag="ACHK1J_002057"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO7911328.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEACGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNDIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16843..18144)
                     /gene="ybbY"
                     /locus_tag="ACHK1J_002058"
     CDS             complement(16843..18144)
                     /gene="ybbY"
                     /locus_tag="ACHK1J_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO7911329.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(18201..19562)
                     /gene="allB"
                     /locus_tag="ACHK1J_002059"
     CDS             complement(18201..19562)
                     /gene="allB"
                     /locus_tag="ACHK1J_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO7911330.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(19622..21076)
                     /gene="ybbW"
                     /locus_tag="ACHK1J_002060"
     CDS             complement(19622..21076)
                     /gene="ybbW"
                     /locus_tag="ACHK1J_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO7911331.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(21245..22123)
                     /gene="glxR"
                     /locus_tag="ACHK1J_002061"
     CDS             complement(21245..22123)
                     /gene="glxR"
                     /locus_tag="ACHK1J_002061"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7911332.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(22224..23000)
                     /gene="hyi"
                     /locus_tag="ACHK1J_002062"
     CDS             complement(22224..23000)
                     /gene="hyi"
                     /locus_tag="ACHK1J_002062"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO7911333.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(23013..24794)
                     /gene="gcl"
                     /locus_tag="ACHK1J_002063"
     CDS             complement(23013..24794)
                     /gene="gcl"
                     /locus_tag="ACHK1J_002063"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO7911334.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24884..25699)
                     /gene="allR"
                     /locus_tag="ACHK1J_002064"
     CDS             complement(24884..25699)
                     /gene="allR"
                     /locus_tag="ACHK1J_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO7911335.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNSNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(25777..26259)
                     /gene="allA"
                     /locus_tag="ACHK1J_002065"
     CDS             complement(25777..26259)
                     /gene="allA"
                     /locus_tag="ACHK1J_002065"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO7911336.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            26489..27415
                     /gene="allS"
                     /locus_tag="ACHK1J_002066"
     CDS             26489..27415
                     /gene="allS"
                     /locus_tag="ACHK1J_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO7911337.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            27484..28578
                     /gene="mnmH"
                     /locus_tag="ACHK1J_002067"
     CDS             27484..28578
                     /gene="mnmH"
                     /locus_tag="ACHK1J_002067"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO7911338.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHAAGIDYPLVAGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            28694..29059
                     /gene="ylbG"
                     /locus_tag="ACHK1J_002068"
                     /pseudo
     CDS             28694..29059
                     /gene="ylbG"
                     /locus_tag="ACHK1J_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29826..30061)
                     /locus_tag="ACHK1J_002069"
                     /pseudo
     CDS             complement(29826..30061)
                     /locus_tag="ACHK1J_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7490 a         8049 c         7407 g         7115 t
ORIGIN      
        1 gaacatgatt tcgatttaca ttgccaccat accgcttcag acaaccggta cattactttg
       61 ttataagacg tgatatataa ttcaaaacaa cgtggttttg attattcgta tctcataata
      121 aaagtcgcgt cagcatttgc ttgccctgct gatgcacttg taccggtgcc tttataacgt
      181 gcagaaaaat gaagaacgtt ggtcccgggg atcaagttct ggttggttga gaagctattc
      241 ccatcaggtt tgagaattgc ggatgtatta tcaagtattt caataccgac acccgttgct
      301 gttgttgtat tggtactgga tgcaatggcc aataaattat cattgattgc atcagcctga
      361 ccagaaaatg caacggcagc attagcggca acaacaggat cgcagtcgtt caactgtata
      421 gtgaatggaa ttaatgcaga ggtattacca acagcattga aaatatcggt acgatattga
      481 ccgagtgtga caacctggtc tgccgagtca gtattcactg aacaggcagc attcaccagt
      541 tcgccttcaa agtgaattgt accgccatct acaacggcag cataactacc cgtagcggtg
      601 aatagtgcgg cagccagcgc agacgaaata aatcttaatt tcatatatat tccttcaatc
      661 tcatttatcg actccacatc cgtatataac cgattacttt atttaagaca ctgatagtag
      721 taaattcctt tttatcctct aagaatgtct taattgaaaa tatgcactct attctaaaaa
      781 atagagagcc ccgttagatg aatacttccg cgcaaaatat attcaacaca aatatagacc
      841 tgaagcggta aattaccagg ctgaaaattc tttttatatt gtcaggtatt tcttaaatta
      901 tcttaatcct ttgacaagga aataagtcag ttccagattt acaacgccat catggacgaa
      961 aaatgaagct ttcaatctca gcgacctcag cgacggtgcg cctaaccttc gcaagaggcg
     1021 cttcacgcga taaatctgaa acgaaacctg acagcgcgcc ccgcttctga caaaataggc
     1081 gcatcccctt cgacctacgt aacagatgga atcctctctc tgatggcagc aaagattatt
     1141 gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt tcaggcgcgt
     1201 attgcagccg gactgcgggc accaggactg gccgttgtgc tggtgggtag taaccctgca
     1261 tcgcaaattt atgtcgcaag caaacgcaag gcttgtgaag aagtcgggtt cgtctcccgc
     1321 tcttatgacc tcccggaaac caccagcgaa gcggagctgt tggagcttat cgatgcgctg
     1381 aatgccgaca acaccatcga tggcattctg gttcaactgc cgttaccggc gggtattgat
     1441 aacgtcaaag tactggaacg tatacacccg gacaaagacg tagacggttt ccatccttat
     1501 aacgtcggtc gtctgtgcca gcgtgcgccg cgtctgcgtc cctgcacccc gcgcggtatt
     1561 gtcacgctgc ttgagcgtta caacattgat accttcggcc tcaacgccgt ggtgattggc
     1621 gcatcgaata tcgttggccg cccgatgagc atggaactgc tactggcagg ttgcaccacc
     1681 accgttaccc accgtttcac taaaaatctg cgtcaccacg tagaaaatgc cgatctactg
     1741 atcgttgccg tcggcaagcc gggctttatt cctggtgact ggatcaaaga aggcgcaatt
     1801 gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga cgtcgtgttt
     1861 gaagacgcgg ctaaacgcgc ctcatacatt acgcctgttc cgggcggcgt tggcccgatg
     1921 acggttgcca cgctgattga aaacacgcta caggcgtgcg ttgaatatca tgatccacag
     1981 ggtgagtaac atggcgacat tttctttagg taaacatccg cacgttgagc tgtgcgactt
     2041 gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga ttgccgaagg
     2101 ccaggtgaaa gtcgacggcg cggttgaaac gcgcaaacgc tgcaaaatcg tcgccgggca
     2161 gacagtgagt tttgcaggtc acagcgtaca ggttgttgcc tgatcccttt cccggcctgt
     2221 atcaacaggc cggttctctt ttcagacttt tgccctctac gccccgttca tccatgctga
     2281 tagcatcgac attttgccga agcgaccatc atgccaaccg ttattacgca cgccgccgtt
     2341 cccctatgca ttggtttagg gttgggttcg aaagtcatcc ctccgcgtct gttatttgcc
     2401 ggaatcatcc tggcgatgct gcccgatgcc gacgtcttgt cgtttaaatt tggcgttgct
     2461 tacggcaatg tttttggtca tcgcgggttt acccattcgc tggtgtttgc gtttgttgtc
     2521 ccgctgttat gtgtgctgat tggacgtcga tggttcaggg ctgggctgat tcgctgctgg
     2581 ctgtttttaa ccgtctcgtt gctatcgcac agcttgctgg attcggtaac cactggcggt
     2641 aaaggggttg gctggctgtg gccgtggtca gatgaacgct ttttcgctcc ctggcaggtg
     2701 attaaagtcg cgccgtttgc attgtctcgt tacaccacag cgtacgggca tcaggtgatt
     2761 atttctgaat tgatgtgggt gtggttgccg gggatgctac tgatgggaat gttgtggtgg
     2821 cgcagacgat aaccggatgc gaaaactcgc atccggcaat agcgcaatta cttacgacgc
     2881 caggtggtcc cttgcgggcc atcttccagc acgatcccca tctcgttaag acggtcacgc
     2941 gccgcatctg ccgccgccca gtctttcgct ttacgggcat ccagacgctg ttgaattaac
     3001 gcttcaatct cagccacttc gctgtcatct gcctgcgcgc cgctttgcag gaacgcttcc
     3061 ggttcttgct ccagcaggcc caatacagcg gaaagtttac gcaggtgaga tgccattgca
     3121 ttcgccgctg ccatatcttc tgctttcaga cggttcactt cacgcgccat atcaaacagc
     3181 acggaatagg cttccggggt gttgaaatcg tcgtccatcg cttcaataaa gcgcgcttca
     3241 aacgcttcgc caccggcagg cgcaacggtt ttatctgtgc cgcgcagcgc agtgtagaga
     3301 cgctccagcg ccgcacgcgc ctgcttcagg ttctcttcgc tatagttcag ctggctgcga
     3361 tagtggcccg acatcaggaa gtaacgcacg gtttccgcgt cgtagtattt cagcacatcg
     3421 cgcacggtaa agaagttacc cagcgatttg gacatcttct cgcggtcaac catcaccatc
     3481 cccgagtgca tccagtagtt cacatactgg ccatcatggg cacaggtgga ctgcgcgatt
     3541 tcgttttcgt ggtgtgggaa catcaggtca gaaccgccgc cgtggatatc aaagtggtta
     3601 cccagctgct tgcagttcat tgccgaacat tcaatgtgcc agccaggacg gcccgcgccc
     3661 cacggagacg gccagctcgg ttcgccctct ttcgacatct tccacagaac gaagtccatc
     3721 gggttgcgtt tgtcgtcgac cacgtcaacg cgcgcgcctg cctgcagctg gtcgagatcc
     3781 tgacgcgaca gcacgccata agttggatcg gtcgggacgt cgaacatcac gtcgccgttg
     3841 tccgccacat aagcgtgacc tttggcgatc agttgttcag tgagttcaat aatttctgcg
     3901 atatggtgcg tcgcgcgcgg ctccatatcc gggcgcagaa tgttcaacgc atcaaaatct
     3961 ttgtgcattt cggcgatcat gcgatccacc agcgccacaa agctttcgcc attttcattg
     4021 gcgcgtttga tgattttgtc gtcgatatcg gtaatgttgc gcacatactt cagcttatag
     4081 ccgaggaaac gcagatagcg cgcaaccacg tcaaaagcaa caaaggtacg cccgtgaccg
     4141 atatgacaga gatcgtaaac ggtgattcca cacacgtaca tgccgacttc cccggcgtga
     4201 ataggcttaa attcctcttt ttggcgtgtc agagtattga agatttttag catcgaagat
     4261 tccgtttaga catgtgtggg taattgagtt gcgtataata cccatatttc ccgcgcgaat
     4321 cagcatacat tgcgtgatga tccgatcgtg cggttatgct ataacaccac cctatatatg
     4381 acccgaactg ggttgaagca ccaatcaaac ggaacaggat gcaaaaatgg ttactttcca
     4441 caccaatcac ggcgatattg tcatcaaaac ttttgacgat aaagcacctg aaacagttaa
     4501 aaacttcctg gactactgcc gcgaaggttt ttacaacaac accattttcc accgtgttat
     4561 caacggcttt atgattcagg gcggtggttt tgaaccgggc atgaaacaaa aagccaccaa
     4621 agaaccgatc aaaaacgaag ccaacaacgg tctgaaaaat acccgtggta cgctggcaat
     4681 ggcccgtact caggctccgc actctgcaac tgcacagttc ttcatcaacg tggttgataa
     4741 cgacttcctg aacttctctg gcgaaagcct gcaaggttgg ggctactgcg tgtttgctga
     4801 agtggttgaa ggcatggacg tggtagacaa aatcaaaggt gttgcaaccg gtcgtagcgg
     4861 tatgcaccag gacgtgccaa aagaagacgt tatcattgaa agcgtgaccg ttagcgagta
     4921 atcgtggcga cactctttat tgcagatctt catctctgcg tggaagaacc ggcgatcacc
     4981 gccggttttc tgcgtttttt agcgggggaa gcccgcaatg ccgacgcgct gtatattctt
     5041 ggcgatctgt ttgaagcgtg gattggcgac gacgatccca acccactcca tcgccagatg
     5101 gcggcagcga tcaaagcggt gtccgattcc ggcgttccct gttatttcat tcatggcaac
     5161 cgtgattttc tgctcggcaa acgctttgcc cgtgaaagcg gcatgacgtt attgccggaa
     5221 gaaaaggtgc tcgaacttta tggtcgtcgg gtgttgatta tgcatggcga cacgctgtgc
     5281 accgatgacg cgggttatca ggcttttcgc gccaaagtcc acaaaccctg gctgcaaacg
     5341 ctattcctcg ccctgccgtt gtttgtgcgc aaacgcattg ccgcgcgaat gcgcgcgaac
     5401 agtaaagaag ccaacagcag taaatcgctg gcgatcatgg acgttaacca aaacgcggtg
     5461 gtcagtgcga tggaaaaaca tcaggtgcaa tggctgatcc acgggcatac ccatcgcccg
     5521 gcggtacatg aacttatcgc caaccagcaa cccgcttttc gcgtggtact cggtgcctgg
     5581 catacggaag gttcgatggt gaaagtcacg gcggatgacg ttgagctgat tcattttccg
     5641 ttttaaaaaa cccgcaactt tgctgatttc acagccacgc aaccgttttc cttgctctct
     5701 ttccgtgcta ttctctgtgc cctctaaagc cgagagttgt gcaccacagg agttttaaga
     5761 cgcatgtctt cccgcaataa tccggcgcgt gtcgccatcg tgatggggtc caaaagcgac
     5821 tgggctacca tgcagttcgc cgccgaaatc ttcgaaatcc tgaatgtccc gcaccacgtt
     5881 gaagtggttt ctgctcaccg cacccccgat aaactattca gcttcgccga aagtgccgaa
     5941 gagaacggtt atcaggtgat tattgcgggc gcaggcggcg cagcgcacct gccaggcatg
     6001 attgccgcca aaacgctggt gccagtgctg ggcgtgccag tacagagcgc cgcactgagc
     6061 ggtgtcgata gcctctactc catcgtacaa atgccgcgcg gcattccggt gggtacgctg
     6121 gcgattggta aagctggcgc ggcaaacgcg gcgttactgg cagcacaaat tcttgcgact
     6181 catgataaag aactgcacca gcgtctgaat gactggcgca aagcccagac cgacgaagtg
     6241 ctggaaaacc cggacccgcg aggtgcggca tgaaacaggt ttgcgtcctc gggaacgggc
     6301 agttaggccg tatgctgcgt caggcaggcg aaccgttagg cattgctgtc tggccagtcg
     6361 ggctggacgc tgaaccggcg gcggtgcctt ttcaacaaag cgtgattacc gctgagatcg
     6421 aacgctggcc ggaaaccgca ttaacccgcg agctggcgcg ccatcctgcc tttgtgaacc
     6481 gcgatgtgtt cccgattatt gctgaccgtc tgactcagaa gcagcttttc gataagctcc
     6541 acctgccgac cgcaccgtgg cagttacttg ccgatcgcag cgagtggcct gcggtgtttg
     6601 atcgtttagg tgagctggcg attgttaagc gtcgcactgg tggctatgac ggacgcggtc
     6661 aatggcgttt acgcgccgat gaaaccgagc agttaccggc tgaatgctac ggcgaatgta
     6721 ttgtcgagca gggcataaac ttctctggtg aagtgtcgct ggttggcgcg cgcggctttg
     6781 atggcagcac cgtgttttat ccgctgacgc ataacctgca tcaggacggt attttgcgca
     6841 ccagcgtcgc tttcccgcag gccaatgcac agcagcaagc gcaagccgaa gagatgctgt
     6901 cggcgattat gcaggagctg ggctatgtgg gcgtgatggc gatggagtgt tttgtcaccc
     6961 cgcaaggtct gctgatcaac gaactggctc cgcgtgtgca taacagcggt cactggacac
     7021 aaaacggtgc cagcatcagc cagtttgagc tgcatctgcg ggcgattacc gatctgccgt
     7081 taccgcaacc agtggtgaat aatccgtcgg tgatgatcaa tctgattggt agcgatgtga
     7141 attatgactg gctgaaactg ccgctggtgc atctgcactg gtacgacaaa gaagtccgtc
     7201 cgggccgtaa agtggggcat ctgaatttga ccgacagcga cacatcgcgt ctgtccgcga
     7261 cgctggaagc cttaatcccg ctgctgccgc cggagtatgc cagcggcgtg atgtgggcac
     7321 agagtaagtt cagttaacct ggtgctcatt tgattgccgg atgcggcgtg aacgccttat
     7381 ccggcctaca ccgctccggt acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc
     7441 aggcatggtg caccgattgc tggatgctct catcactaca gcgaaataca ggtccccgct
     7501 tcgcccgcca gcgtctcttc aattcgcgat aacgccccaa tccacgcggg tttaccacgg
     7561 cttctgacat aaccactcac cgccgttacc ttcggcccca tcgaaccatc ggctttggca
     7621 aatggcgcta actcatccgg tgtggcatgg cgaatggcac gttgctgcgg cgttccccag
     7681 ttttcatata ccgcatcagc atcggtgagg atcaccagtc catcggcatt aatctgctcg
     7741 gcgagcaacg cagcagcgag atctttatca atcaccgctt cactccctgc cccgtcttcc
     7801 gtcacaggca caccgccgcc gccactgcaa atcaccacat gcccctcttt gagcaacaac
     7861 tcgatggctt cgctgtcgag aattttacgc ggttgcggag acgccaccac ccggcgcaaa
     7921 tatttaccat cacgtttcat ctgccagccg taagccgctt ccagtgcctc ttgttcttct
     7981 ggctgataaa ccggaccaat aaatttctct ggctgcaaaa acgccggatc atcaggcgaa
     8041 acctcaatgc gcgtcagcac cgtcgtgaca ggcggcatct gcggctgtgc gctcaaactc
     8101 tgcgccagca tatagccaat catcccctgg ctttccgcca ccagcacatc cagcggatac
     8161 ggttctacct ctttccacgc cagattctgt aacgccagca gccccacctg cggcccgttg
     8221 ccgtgaacaa tcgccagccg ataagaacgg gccaggcgcg ccagcgcggg tacagcactg
     8281 gcgatattgc gatattgatt ttctgccgtc agcgcctcac cgcgctgaag caaggcattg
     8341 cccccaagag ctacaaccag tgttttcatg gttttccttg taataattgt tgaccaagcc
     8401 agaaccccag cagtgtatct gcccctgacg tatgccccag cgccagcagc gaatcaatcg
     8461 caacagcggt acgtttcggg caactcagag catgaacaaa gtgcaggagt ggcgaggcga
     8521 aatatccttg cgcggcataa cgtaaataac tgacgctcac cgctgtggta acgagttgca
     8581 gattgtcgga acaggcaaaa aacggacgac cggagcgcgc atctaaagcg ccataatacc
     8641 aggccgccag cagcattccg ctcaacgtgt catcatgact tggcgttaat ccggggcctt
     8701 tacccagcca gtgccgccag tcggtcttaa cgccattgag cgcggcctga aaacagtgac
     8761 gaaactggcg taattcagcg ggcagcggat cgcttgccgc catcgccagt ggcccgaaaa
     8821 gtccggtttc ctccgcgcgt tgcatccatg cagctgcaag cggttgagga tgcgaaggcg
     8881 gcgtaatacg cagcaaacaa taacgctgtg gctgtgtaac cgtgaaacgc ccgaggcgaa
     8941 tcccttgagc cacaacctgt ggtcgttcat tgccgcataa tccaccgcat aacgcatcga
     9001 attgcgcacg gcgaagcacc catcctccgg ggccgaaacc actaccctga cgatgcagcg
     9061 ttaacagttc gccgctttcc gtcatcaggt taatcgcccg ccgccacact cccgctaacc
     9121 gccaggactg gcgataattc ggtgcgctac tactggcaag cagaggatgg atgatcgtca
     9181 ttgcgcgttc ctcaaccaat acccatgctt tcagccagtg cttccagcgc ctgttcaaag
     9241 cacgccagcg gtgcccgcac ggtgcctgcg ccaatctgcc cgatccccgc ctctttatgg
     9301 gcgataccgg tattgatgag tggcgtaatg ccggtttcta ccacgcgacg aatgtccagt
     9361 cccaggcacg cgccctgaaa atcccagctt gggatctgca actgcatatt gcgttcaagg
     9421 taaatttccg ccatctcttc agataccgcc ctggccgctt ccatgccacc cgcaccgaca
     9481 aagcgcgtta cgccaggcgc tgcgatcatt gccgcacctc cgataccaaa ggtttcggta
     9541 atcgcgctat cgcccatatc cgggttcgcc tgctcctgcg agaagccggt gaaaaacaga
     9601 ccttgcggag tgtttacagg cgcagtaaac cagcgttcgc ccagcccgct tacccgaatc
     9661 ccgaacatat tgccgttgcg ggtcattgcc gtgacgatgc tgcctgcgcg gatcatcgcg
     9721 ccagcatcca tcgccgcctt gcagtaagcc atcgcgaggt tgaggaagaa ctgatcggtc
     9781 acgctgagga aatccatcac ttccgcgatg tgctgtttat catgatcgag gcgagcaatt
     9841 tgtggggcca gcgcacgcat taacagtgct gaggaagcaa tattgcgttg atggaactca
     9901 tcgcccatcg taatgccctg cgccatcatc gcggtaagat cgataccgcg ttccatgcgc
     9961 cccagcgccg cgcttaatac tggcattaac acatcgcgca tccagcgatg gcgagtcagg
    10021 acatcttcgc cgtaagcgcc aaaacgcatc actttgccga taccttcgtt gaggttgcag
    10081 tacgcccggt tgccgtcggt cacgttctcg accaccagca tcggcatact ggcagaagta
    10141 ataccgccca ttggcccgac ggcattcaca tggtgacaag gaatgaagtt cacttccccc
    10201 tgctccagta ttgccagcgc ctgcgcttca tctttcgccc agccttcgaa cagacatgcg
    10261 cccacgcacg cccctttcat gggtccggtc atctcctgcc agcgcattgg cggcccggcg
    10321 tgaagcagtg ttttgccctg gtttagttcg ctgataagtg aagaagccgg ttgcacatcc
    10381 agccagtgtg gacgagcgcg acgaatttgt tcgataaccg cagcattggc ttgcgccact
    10441 gatgtaaaca tgggaacccc ttattgcaaa cgttctaata aacgagccag ttttttattg
    10501 ccaccggcga ctggcgacca ttggtaatgc acaaccggtt tgctggcgct ttgtagctcc
    10561 agcgcaaaac tgcgtaatcc gatgttaatc acggcgacgt tttccaataa tgacggtgtg
    10621 tgttgctgtg cggcaggcga gagcggatga attaacgctg ccgccagcaa ggtggcttcc
    10681 ggtagcgaac tcacgaccgc aatccccgca tcttccagcg tggcgatttg ctgcgagcgg
    10741 cattgcgggt cacgttcagt gcctgtcacc gtggcaatgg catacagtgg ttgattgtct
    10801 gaacgcgcag cacaggcttt ttgccaggcg cttaccagcg aggcggcagg atcggcagtc
    10861 gcaccgaagc caatcacgac atcaagcagc agcacacgca cttgcggttt agcgccgaga
    10921 tcggcaatta actggttgcg taaggttggg tcgatcatcg gatggggacg cccgacggtg
    10981 tagaaatcat cgccgaggtc aataatctgg tggccatcgg cgtccagcat catgccgtga
    11041 tgatgggcat catcggcttc cacgccaagg tttccggcaa gtaatcccgc cgcttcggca
    11101 gccagcgtac cgccggtata caaaccgcaa ataaatccgc tgctggcagg cgttatcgcg
    11161 ttacgtcgcg ccgtaacgcg tgaaagcagg caagccaggc gtgcggcctc atccagcgag
    11221 gaggcaaacc agacattctc gtcgcgggcc accgccgggg tataacctaa acacagcgcc
    11281 accgtcggtt tgccggttgc tttcatggca ttaacaattt tcagacgcac cgcttcggca
    11341 ggtggttttg aaacaaatgc cagcacttcg cttttctcgt ctgcactgag catttccagc
    11401 gccgtcagcg cactgatgcc gcccacttca cggctgaggt cgcggccgcc aaggccaatc
    11461 gcgtgagtaa ttccctcccc tgccagcgca atctgcgagc acagctcctg aatcccggta
    11521 ccggaagcgc caatgacgcc aatgttgcct tccggcatca cgttagcaaa agccagcggc
    11581 gtgccggcaa tcatcgacgt accgcagtcc ggccccatca ccagcaagcc tttttcccgc
    11641 gcgcgggttt taagttggat ttcatcttcc agcgtgacgt tatcggagaa catcatcacg
    11701 ttgaggttgc gatccagcgc ctgattcgcc agctccgccg catactcgcc agccactgaa
    11761 atcagcgcca gattggcatc gggtaatttc tgacaggcac tgtcccagcg acgcacctgc
    11821 gtcaacgcct ggctgctgcc tgccccctgc gccagttgtt ttagcgcctc ttcaagctgc
    11881 tgcataatcg cctgcgcgat ccccgcatcc gccgcttcgc tacgaattgc cacgcaaata
    11941 tcgttcggcg tggcgttatt aaaatcgtca tgcctgaaac ctgtggtatc taataacgct
    12001 ttattggcgg gcgtacccat cattacggaa acatcatcaa cattttctga ttcgctgagt
    12061 tttcgtgaaa taatcattaa actgaccgaa tcctgaaaac accctttttt aataaaggcg
    12121 tggatcataa caactcctta aaaattgcac ccatggcaat tgcacgagca gtgttaatgt
    12181 ggcgattacg gtatcgatga caacgggtaa ataaggtgat gaatatcaca cagggattat
    12241 taacaaaaga attcaaataa ccgccatgct attacgtgtt attttttaat aagcgtggtt
    12301 gttttaaaaa ccaggttaaa agcgagcgga ttatcaaata aattctaact atattttttt
    12361 gcctgtctgg atcacataat ccagatattt tccctgtatg ttaattgcag tcatgcttca
    12421 caccgtcgtt aaaaaggaag acagatgaaa atcagtcggg aaacactcca ccagctaatt
    12481 gagaataaac tctgccaggc tgggttaaaa cgtgagcacg ctgcaaccgt ggctgaagta
    12541 ttggtttacg ccgatgccag agggatccac tctcatggtg cggtgcgcgt ggaatactac
    12601 gcggaacgca tttcaaaagg cggcaccaac cgcgaaccgg agtttcgtct tgaggaaacc
    12661 gggccgtgct cggcaatttt acatgccgac aatgccgccg gacaggtcgc ggctaaaatg
    12721 ggtatggaac atgccatcaa aaccgcccag caaaatggcg ttgcggtggt cggtatcagc
    12781 cggatgggtc acagcggcgc aatctcttat tttgtgcagc aggcagcccg cgccggatta
    12841 attggcattt cgatgtgcca gtccgatcca atggtggtgc cttttggcgg cgcggaaatt
    12901 tactacggta ctaacccact ggcctttgcc gcgccgggag aaggcgacga gatccttacc
    12961 tttgatatgg cgactaccgt acaggcatgg ggaaaagtgc tcgacgcccg ctcgcgtaat
    13021 atgtctatcc cggatacctg ggcggtcgat aaaaacggtg caccaacaac cgatccgttc
    13081 gcggtacatg ctctgctccc cgcagccggg ccgaaagggt atggcctgat gatgatgatt
    13141 gacgtcctct caggcgtctt actcggctta ccgttcgggc gacaggttag ttcgatgtat
    13201 gacgatttac acgccgggcg taatttgggg caattacatg tagttattaa cccgaacttt
    13261 ttctcctcca gcgaattatt ccgtcaacat cttagccaga ccatgcgcga attaaatgcc
    13321 attacccccg cgcccggttt taatcaggtt tattatcccg gacaggatca ggatattaaa
    13381 caacgcaaag ccgccgtcga aggcatcgaa attgttgatg atatttacca gtatttaatt
    13441 tccgacgcgc tttataacac gtcatacgaa acgaaaaatc cctttgcgca ataattattg
    13501 agacaggact ttcttatgat tacacatttt cgtcaagcca tagaagaaac gctaccctgg
    13561 ctttcctcat ttggcgctga cccaacgggt gggatgaccc gtttacttta ttcaccggaa
    13621 tggctggaaa cccagcagca atttaaaaaa agaatggcag caagcgggct ggaaacacgt
    13681 ttcgatgaag tggggaattt atacggtcgc ctgagtggca ccgaatatcc acaggaagtg
    13741 gttctgagcg gttcgcatat cgataccgtg gttaacggcg gtaacctcga cgggcaattc
    13801 ggcgcgctgg cggcgtggct ggcaattgac tggctggaaa cgcaatacgg cgcaccgctg
    13861 cgtacggttg aagtggtggc gatggcggaa gaagaaggca gccgcttccc gtatgttttc
    13921 tggggcagta aaaatatctt tgggctggcg aatcctgacg acgtgcggaa tatctgtgat
    13981 gccaaaggaa atagttttgt cgatgcgatg aaggcttgcg gatttactct gccggacgcc
    14041 ccgctaactc cgcgtcagga tattaaagcc tttgtcgaac tgcatatcga acagggctgt
    14101 gtgctggaaa gtaatgggca atcaattggc gtggtgaatg caattgtcgg gcaacgtcgc
    14161 tatacggtga cgctgaacgg cgaatcaaac catgcaggca ccacgccgat gggttatcgt
    14221 cgtgatacgg tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag
    14281 atgggcgacc cgctggtgct gacctttggt aaggtggagc cgcgcccgaa tacggtgaat
    14341 gtggtgccgg gtaaaaccac attcaccatt gattgtcgtc ataccgacgc cgccgtgctg
    14401 cgcgatttca ctcagcagtt agaaaacgac atgcgggcga tttgcgatga aatggacatt
    14461 ggtattgata tcgatttatg gatggacgaa gaacccgtgc cgatgaataa ggacctggtc
    14521 gccaccctga cagaattgtg tgaaagtgaa aaactgaatt accgggtgat gcacagtggt
    14581 gccgggcacg acgcgcagat tttcgcgcct cacgtgccga cctgcatgat tttcattccc
    14641 agcatcaacg ggatcagcca taacccggcg gaacgcacca atattaccga ccttgccgaa
    14701 ggggtcaaaa cgttggcact catgctttat caacttgcct ggcagaaata aggagtcata
    14761 aatgggatat ttaaataacg tcaccggtta ccgcgatgat ttactggcta accgtgcgat
    14821 tgtgaaacac ggtaatttcg cactgttaac accagacggt ctggtaaaaa atattattcc
    14881 gggctttgaa aattgtgacg cgacaatcct ctccacgcca aagctgggtg cctcttttgt
    14941 tgattatctg gtcacactgc atcaaaacgg tggcaaccaa cagggcttcg gtggcgaagg
    15001 cattgaaacg ttcctgtatg tgatctctgg aaatatcact gccaaagccg aaggcaaaac
    15061 atttgcctta agcgaaggtg gctatcttta ttgcccgcca ggctccttaa tgacgtttgt
    15121 taacgcccag gccgaagaca gccaaatctt tttatataag cgccgctatg ttccggtaga
    15181 aggccatgca ccgtggctgg tttctggcaa tgccagcgaa ctggaacgca ttcattatga
    15241 aggtatggat gatgtgatcc tgctggattt tctgcccaaa gagttaggtt ttgatatgaa
    15301 catgcatatc ctctcttttg caccaggtgc cagccatggt tatatcgaaa cacacgttca
    15361 ggaacacggt gcctatattc tttccggtca gggggtttat aacctcgaca ataactggat
    15421 cccggtgaaa aaaggcgatt acatctttat gggcgcttat tctttacagg ctggttatgg
    15481 cgtagggcgt ggtgaagcgt tcagctatat ttactcgaaa gattgcaacc gcgacgtaga
    15541 gatttaagtc ataccactcg cctgattatt tttagctatg ttggattttg ccacggttta
    15601 taccgtggct ttttttgtag taaattcagg taaaaaaaga tgcgatataa atcgtatccg
    15661 ctttaaaggt aagggtcagt ttttaattcc ctgacctatt ttaatggcgc aggcaatatt
    15721 tcgcgcgctg ttgaagagat tggtttcacc gctggcgagc acttcggcta aaggtgccag
    15781 acgaggcaaa atgctgaata ccgcgtcaat gccgtactgg tgcaccactt ccacgccatc
    15841 acccaatacg ccagcaatcc caatcaccgg tacattaaac tgcttcgcca ccgacgcaac
    15901 gcccagcggc gctttaccgc ctgccgtttg cgagtcaatg cgcccttccc cggtgatcac
    15961 cagtgctgcc ccctgcaccg cctgctccag gttgaccgca cgcagtacaa tctcaatacc
    16021 cggtttaata tccgcattga gaaataccgc cgccgcaata cccataccgc ccgcagcgcc
    16081 gccgcccgcc atctgacaga cattaatttc agtttgctgt tgtaaaacac gggcgtaatt
    16141 ttgcagcccc tgttccagct cttcgaccat ctccggcgtt gccccttttt gcgggccaaa
    16201 caccgccgcc gcgccgcgtg cccctaccag cgggttatca acgtcacatg ctacttcaat
    16261 atggcagttc gccagacgcg gatcgcattc ctccatctca atgcttgcca cgcgcgctaa
    16321 attgccgccg ttagctgcca gcacctgacc gtcggcatcg agaaaacgca caccaagcgc
    16381 ctgcgccatg cccataccgc cgtcgaccgt cgcactgccg ccaatgccga gaataatatg
    16441 gcgaatgtcg ttatccagcg catgccgaat taactccccc gtaccaaaac tggaggccag
    16501 caacggatta cgcttttcag gcgcgaccag catcaggcca cttgctgccg ccatctcaat
    16561 caccgctgtt ttcccgtcgc cggtaaggcc ataaaaagca ttcacttttt cgcccatcgg
    16621 cccgcaggct tcaagcgtca cgatgttacc acccgtcgcg gcgaccatcg catccaccgt
    16681 cccttcgccg ccatccgcta tcggcaaaca gatatagttc gcatcgggaa agagggtcga
    16741 aaacccggct ttaattgcct gacaacattt ttctgcactt aagctctctt taaaagagtc
    16801 tggcgcaatg acaatcttca ttatcaatcc tttttcaaca aattattcga tgcgttccca
    16861 cggaataagg ttatccataa gaacagccag taaaatcccg accaataagc cgttactgag
    16921 cagaggacga agcgttaatg gaaggtcttg cagatacaca ggcggtaatg ccattaaaaa
    16981 tatgccgaca aataacggca atgcgagtcg ataaatattg cgagcggtaa acgttatttg
    17041 ctggctaaac accagcgcgg aaaagagtaa aggcagataa gaaaccagca tgaccgcact
    17101 actcacgggt aaagggatac tgcaaaacag tcgcgtgagt gccggaacca gcgccaccag
    17161 caggcaaata acgctgccat aaataaacga acgccgcgtg taatcgccag tctgggttaa
    17221 taaaccaatg gatgaaacaa acggtgaaaa tggaattacc gccagcggta cggttatcag
    17281 tgtcataaat ccggtcgcca caaagctacg acgataacgc gtattccccg cgccctgctg
    17341 cggataaaaa acatccgtgc cccgaatcgc accgtaggta ttgctgatat ttaccagacc
    17401 tgtaatcacc gccgtcagaa taattcccgg cgacaaagca ccgccactgc cgagcgggaa
    17461 ccactgccag tgcaactcac cggagagcga atgcgaagaa ggaaagcaaa agtaccacaa
    17521 caaccagccg gttatggtgc cgaccagcag gccataacgg gcaaaacgtt gcggcagaaa
    17581 gataatcatc gccagtacca ggcacatcac cgccaccgag agcgcgaatg gtggtaactg
    17641 aattttaaaa tttgggtcgg ctatgccaaa cggcagcccg agcatacctt tgaaaaagat
    17701 agtggtcagc tgtgcgccca gcatcaacat aaacaagacc atcaccgacg gcgtaaataa
    17761 ccgtgccagg cgatggccta atccgctaaa accaatcaac atcgtcagca cgccggagag
    17821 cgcaatcccc actgccaggc tggtggcgat atcgttgatc ggtgtcccgc gcgatgcttc
    17881 gccaagggtg atagttagga tggttcccca ccacaggccc cccggccctt ccataatagc
    17941 gcgacgatgg ccacagaacg cctgagcgaa gcaggccagt gcggtagcaa gaaaagcata
    18001 ttgtgtgagt gtaagcaggc tactttgcgg caactgaaaa gcagaaagta gcgtaggagg
    18061 aaccacaacc gtgttgcaaa aaataaagaa aaaccactga aatcctgata acaggctttc
    18121 gcggctgact gcaaaattga acataaacac accttaaccg gagaatgccc gccgcaagga
    18181 cgggcattgc aggggccaga ttactgctga tgtttaagga taaattgacc tttcggcgca
    18241 acagggaagc cctgttcgat gtcgtaaatc acatcaccac gtaagatggt tttcgtgata
    18301 cgcgcgccaa tggtacggcc aacatacggg ctgactttgt ggcgatattc cagatcgtca
    18361 ttggtaagaa catagctgct attcggctga atgaagacga agtcggcatc ttttcctggg
    18421 gcgatacggc ctttttgctg cagaccgaaa atatctgctg cgttagtcgc cattaatttg
    18481 ccgaacattg gcagagacat tccgcgtttc tgtaccgctt catcgaacat cacgtccata
    18541 cagttttgca gaccggcaat accgccccat gcttccatga tgttgccggc tttcatttcc
    18601 ggagggcatg gtgagtggtc ggaaaccagg cagtcgattt caccgttaaa cagtttttcc
    18661 cacatgcctt tctggttttc cagatcgcgg atcggcggtg aacacttcgc cagagtgccg
    18721 atttcttcga actgatcggt atccagtaca aagtaatgcg ggcaggattc acaagtaaca
    18781 tcctgacctt cctgacgtgc acgagtcact tcttcaacgc cttccgggct gctgatatgg
    18841 caaacgtgca gacggcaacc ggcaactttc gccagataca gcacgcggcg aatcgcttcc
    18901 acttcggtaa ataccggacg cgaagccaca tagtcatggg cagttacgcg accttcacgt
    18961 ttcgcttctt cgcccagtgc gtcacagatc agcgcgtttt cgcagtgcac cagcaccggc
    19021 tgccccagtt cgcccagctt ctgcgcgcct ttgaagaact gccagtcatt tacatcacgg
    19081 aagtcgttgt cgataccgcg atcgccacag gtcgcaacga agcatttgaa gccgacaacg
    19141 cccacttcat ccagctcatg cagacggtcg atgttgtaag acaccaggcc accgagttgt
    19201 gccgcatcga tagtcagctt gcctttagcg gcatcgaact tcagttcaat tgaagcgcgg
    19261 tcaaccgttg caggcagctg gttgagcggc atttcgatca tggtggtgat accacctttt
    19321 gccgctgcgc gagtaccggt ttcataacct tcccagtggc tacgacccgg ttcagaaata
    19381 tgggtgtgcg catcaaccat gcccggcgaa accaccagac cagacgcatc cataacttct
    19441 tttgcatcgc ccagatcctg accgatagca gcaattttgc cgcctttaac ggcgatatct
    19501 acaacgcgag cttcgttttc taaaataacg gtgccgtttt taatgattaa atcaaaagac
    19561 ataacaaact ccttgtaaaa tacagattgg tttttaactc aatgttcaat atcgggatta
    19621 attaacctat ggttttttgc tctcctgttt tttctgctgt tgtacgtttc tttaataagg
    19681 cgtaggccgc aaaggcgacg atgacgccga caaaccatga gacacgcgat aacggttcca
    19741 taaagggaat aaacttaccg ccaagagata aaataacggc caccagagtt actgaaaacg
    19801 cggtgaggtt aaaaccgtta tcgtaatatt tataatcgcc aggtgcggtg tacagttcat
    19861 caagattaat ttgcccacgc atcaccacaa aataatgcgc catcatgaca ccaattaccg
    19921 gaccaagcat tccgccgata atatcgagga acagataaat actgtcctga ttttccatta
    19981 atttccacgg gcagatcagc aggctgataa tactggcaat cagtacgccg tttttatagg
    20041 tcagttttgt cggtgcaatg gcagcaatct gataaccagc cggaataata ttaccagtgg
    20101 cgttagtgga gatagtcgtc atcagaataa ccagtaccgc aaagaacgag gcaaacaggc
    20161 tgtcccaacg ctgaacaata tccagcacgt tccaggtatc agcgccgtaa tgaatactgg
    20221 ctccggcaat aatacatacc ccggcgaccg caaacagaat ataggccaca actaaaccca
    20281 gcgtttgccc cagcgcttgc tcacgaaacg agtgggcgtt ttgcgtaaag tcggatgcgc
    20341 tcaccgccgg agccgcccag accgcaacta ccgcgttaat caccaccagg aacaggaagc
    20401 cactgttttc tgctttctga ataccgctcg gaatgtagtc aaagattgga ccgagcccga
    20461 ccagcgaaat cgcccaaatc gccataccgc cgaaaacgat atagatgcac gggttaagaa
    20521 tggcagtgaa tttatttaaa actttgccac cgccaaaacc tataccaacg ttgaccagcc
    20581 agaagagtaa gaaagtaatt aagcccggta gagaaaggcc taacagagtg aaatcaccac
    20641 cgagagtcaa aaatcccggc cagattttgc caatcagaat caagcaggcc agtgaccccg
    20701 cgtaacattg caggccaaac cacataatgg cagcaatacc gcctcttaat aatccgggaa
    20761 acagtgcgcc acgtacgccg taagaagcac gcaggatcat ggcaaacggt acgccgtatt
    20821 tactgcccgc agcgccgttt aataccatta ccgcggcaat gaaaaaggcg ctgaggataa
    20881 ttgccagcat aatactaaag gtagacaagc cgagaataaa aaagccgccg accatcacat
    20941 aattgggaac gttatgaacc gaacccatcc ataaggtaaa atagttaaat gttttccagg
    21001 tccgctggct ttgcgttttc ggcaatagat cttcgctata gccgcgttgc tggaatagtt
    21061 ttctctgatg ttccataaat ccctcttaat ttcacgtatc gaataacgtt gttttaatct
    21121 agctatgagg aacaaataaa ataaaaatat tatttttaat actgaagcaa catctttggc
    21181 tgctttcttt tttaatattg ctggccgatt cattttttga tatcggccat ttttattgcg
    21241 ggtatcaggc cagtttatgg ttagccatta attccagcgc ctgcactaac gcagagtgat
    21301 ccaactggct gccaccgttt gccgcgcagg tattaaataa ctcctggcag gtcgcagtgt
    21361 ttggcaggtt cagcgccagt gccttcgcac tttgcagtgc caggttgaga tctttctggt
    21421 gcagcgcaat tttgaaacct ggattaaagg tgcgtttaat catgcgctca ccgtgaactt
    21481 ccagaatacg tgaggaagca aagccgccca tcagcgcctg gcgcacacgt accgggtctg
    21541 caccggcttt tgaagcaaac agcagagctt cagagaccgc ttcaatattg agcgctacga
    21601 taatctgatt tgccactttg caggtttgac cgtcgccgtt accgcccaca agggtgatat
    21661 ttttaccgag cagttcaaac agcggtttaa cacgttcaaa taccgcttcg tcaccgccaa
    21721 ccataattga caacgtccct tcacgcgcgc cgatttcgcc gccggagact ggcgcatcga
    21781 gataatcacc acccagttca ttcacctgac gagcgaaacg tttagtttca atcggtgaaa
    21841 tggagctcat atcaacaatg gttttgccct tcagcgaggc tttggtacaa ccattttcac
    21901 caaacagaac ttcttcaacc tgaggtgtgt ccggcaccat aataaaaatg atgtccgctg
    21961 cttccgttac ctggcgcgcg gtgtcgacat taacagcacc gagtgacagt aattcatcag
    22021 caaccggtcc aatggtcgtg acatgtaatt gatgaccggc acgcgccaga ttaatggcca
    22081 tcggagtacc cataatgcct aagccaataa atcccagttt cataattaac ctctttttaa
    22141 atgtcgctat tcctgaattc agacaacacg atcccctgcc aacttagcgg cctaaaaaag
    22201 cattgtctga atagcgttac gttttaacgg tacggatcca tccagcgtaa accggcttcc
    22261 gtggtggttt ggggtttata ttcacaccca acccaaccgt tgtagtcaga attttcgatt
    22321 accttaaaga gataatcata attaatttct ccggtccccg gttcgccgcg atgcggatta
    22381 tcagcaattt gcaggtgacc aattttatcg gcccactgag tcatggtgtt ggttaattca
    22441 ccttccatcc gctgcatgtg ataaatgtca tactgaattt tcaaattgca gcaaccaaca
    22501 tcatcaatca atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg aatatcaaaa
    22561 tggttaatag gttcaatcag taataaaata tcttctttca tcagcatatt cgcggcataa
    22621 cgcaggtttt ctacaagcgt tgcgtgaatc tgttcgctgc tgaaaccagc cggcgtttta
    22681 ccgaccagac agttaatttt tttattaccc agcgcacggg cataacgaat cgctgctgct
    22741 acgccatccc gaaactcttc ttcacggcca ggaatacagg caataccgcg ctcccccgcc
    22801 gcccagtcac ccgccggtaa attgtgcagc gtatgttcga gtttattact cgccagcacc
    22861 tgttttaatt cttcaatgtc gtagtcataa ggaaacataa attcaacgcc gcgaaaacca
    22921 cactgcgcag ctttctcaaa acgggcgaga aaatcatatt caccaaataa catcgataaa
    22981 ttagcagaga aacgtaacat tatttatctc ccttattcat agtgcatgaa gcaggtttca
    23041 gtcggtgcat ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt atccagttcg
    23101 ctgcccatcg aaatatttgt cacacgctcg agaataactt ccacgactac cggtacccga
    23161 tattgcgcca ttaaggcttt cgcctgttca aaggccggtg caatatcttc cggtttgaag
    23221 acgcgaatag ctttacaacc taaaccttcc gctactttta cgtggtcgac gccgtaaccg
    23281 ttcacttcgc tggagttgat attctcgaaa gcgagttgca cgcagtagtc catgtcaaag
    23341 gcgcgttgtg actgacgaat caggccgaga taggcgttat tcaccagcac atggatgtac
    23401 ggaatgttga actgcgcgcc aacggctaac tcttcaatca ggaactggaa gtcgaagtca
    23461 ccggaaatcg ccaccacatt gcgtttcgga tcagcggcac aaacccccag cgccgccgga
    23521 atcgtccagc ctaacggacc agcctgacca cagttgatcc agtggcggtc tttaaagaca
    23581 tgcagcattt gtgccgcagc gatttgtgac aggccaatgg tggtgacata acaaacatcg
    23641 cgaccaaagg ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg cacgttgtcg
    23701 aaatgggttt tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac ccattcttta
    23761 cgacacggca gacgacccgc tttttgcatc tcctgcgcca cttcaaccag cagtgtcagc
    23821 gccgctttag catcagagac aatgccgaga tccggacaca gcacgcgacc aatttgcgtc
    23881 ggctcaatat cgatatgcac gattttgcgc ccttcggtgt atttctctac cgaaccggta
    23941 tgacggttag caaaacggtt accgatacca aacaccatgt cggacgccag cagcgttgcg
    24001 ttaccgtaac gatgcgcggt ttgcagaccc accatcccgg ccatcagttc atgatcgtcc
    24061 gggatacagc cccagcccat cagcgttggg atcaccggaa cgctggttag ttcagcaaac
    24121 tgttgtaaca gtgcagctgc gtcagcattg attaccccgc ccccggcaac aatcaccgga
    24181 cgttcggcct ggattaacat ttctacagct ttttcgatct gcatacggct ggcagcaggt
    24241 ttgtagaccg gcagcggttc gtacatgtca ggatcaaact cgatttccgc aacctgaacg
    24301 tcgaacggta aatccaccag taccggaccc ggacgaccag aacgcatcag gtgaaatgcc
    24361 tgttgcagca cgcgaggcac cagcgccgct tcacgtactg taaccgccat tttgctgacc
    24421 ggtttagcaa ttgcttcaat atcaacagcc tgaaaatctt ctttatgcag acgggcgcgc
    24481 ggagcctggc cggtaatgca cagaatagga atggaatcag cagaagcaga atagagcgcg
    24541 gtgatcatgt ccgtgcccgc aggaccggaa gtccccagac atacgccgat attccctgcc
    24601 gttgcgcggg tataaccttc cgccatgtgc gaagcacctt ccacatgacg cgccagaatg
    24661 tgacgaatac cgccgtgctt acgcatcgct gagtagaacg gattgattgc agctcccgga
    24721 acaccgaagg cggtggtgat accttctttc tccagcacat acattgccgc gtcaacggct
    24781 ctcatttttg ccattttatt cctacctcca ttaattggaa aaattttcca actttcaaat
    24841 tttgtcaaaa aaacccgctc cgcagagcgg gatgcgatag acattatgga tgtgctttca
    24901 gtcccaacgc cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc tgactgacaa
    24961 aacgatcttc tgtcagtctt gatgaaggcc cggagatgga gatagcggca acaacactac
    25021 ccacatcatc gtaaattgct gaagctatgc aattcagacc tacaacatgc tcttctttat
    25081 ctacggtata gcccagttca cgcgcttgtt ccaggtcctt cagcagggtg ggcatatcca
    25141 caagcgtagt tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc atcaactcct
    25201 cttccgccag cggataaagc agcgctttgc ccgcaccgga agcatgcagt ggcagacgac
    25261 tgcccagtgg cgcacacatc ctgaccatcg atttacactc taactgacca attaataccg
    25321 cttcattgct gttacggatc gcgacattga ccgtttcgcc agaaagtaac atcaggcggc
    25381 gcataaacgg cccggcgacg gagaggacat cgcggttatg gatgtacgct gcaccgacgt
    25441 taaagacacc taatcctata tgccaccagc ctaactgact gtcctgatag acaaaatccg
    25501 ctgcctgtaa aaccttcagc aagcgaaagg tcgtggagag cggcaaatcc agattgagag
    25561 aaatatcgct aaccgacgaa cttcccccac ttttttccag atattgcaga atcgcaattc
    25621 cccgctctaa tgcctgtgcg cccttctgtg ccacaggctc cgcctgtcct ggcctgccgc
    25681 gccgtctaac ttccgtcata cctacgttaa ctccgaccta caagtgagca taaacaatga
    25741 acaattagtt tatcttatgc aagtcggttg caggcgtcac gcaaaacaga gttcctgttc
    25801 aggaatactt tcaacatcac agttgtcact gccgccgcga tcgatggtca gaaaatcggt
    25861 gacgcgctgc caggcgaaaa gtgggtgatg ccagacgtta cgatggtaat tcactccctg
    25921 ttcgccgttg gtaataaacg cccgcagggt tgacaggtct ggtttgtcgt cacctaacgc
    25981 cacgaccacc acaaacacct cacctttcat cgggataaag gcctgagtac ccagcggatg
    26041 acgctcgagt tcgtgaatgg tcagcggcag attcgccggt tgcgcgcggt taatgctgat
    26101 aagcgtacgg tcttgctcaa gaatctcaac cagcgccaaa tcgtggtaac gctccaccag
    26161 gccattgtta atatggaaaa aatcccgttg ctgcgtttcg attacgtcgc cataagcact
    26221 aaaggcttcc tgacttaacg gtaatacctg aagtttcatc tttttgtcct tgtaacataa
    26281 ttccgttcaa ccaccgccga ccggtcagct tggtttcaat aacaggcaat ctaaaacctt
    26341 aagagaacaa catcaataca aaagttggaa tatttttcca aaactagatt tagtccacaa
    26401 attgaacagg gttatcatgt aaaaatactg ataaatcaga ggataagaaa atcacgcaac
    26461 ttgcggattt aaaaggggat acctgaagat gttcgatcca gaaaccttgc ggactttcat
    26521 tgcggttgct gaaacaggaa gtttttcaaa agcggcagaa cgattatgta aaaccacggc
    26581 gacgatcagt tatcgcatta aacttctgga agagaatacc ggagtagcgc tgtttttccg
    26641 tacgactcgt agcgtaacgt tgacagcggc tggcgagcat ctactttccc aggccagaga
    26701 ctggctgagc tggctggaaa gtatgcccag cgagctgcaa caggtgaatg atggcgtgga
    26761 acgccaggtg aatattgtca tcaacaacct gctctataac ccccaggccg tcgcccagtt
    26821 gctggcgtgg ctgaatgagc gttacccctt tacccagttt cacatctccc gacaaatcta
    26881 tatgggcgtc tgggactcgc tattgtacga aggtttttcg ctggctatcg gcgtcacggg
    26941 aactgaggcg ctggcaaata cctttagtct tgatccctta ggatcggtgc aatggcgatt
    27001 tgtcatggcg gcggatcatc cgctggcgaa cgttgaagag ccgctaacag aagcgcagtt
    27061 gcggcgcttt ccggcggtca atattgaaga cagcgcccgc accttaacca aacgcgtcgc
    27121 ctggcgattg ccgggtcaaa aagagattat tgttcccgat atggaaacga aaatcgccgc
    27181 ccatctggcg ggcgttggca ttggtttttt gccaaaatcg ctttgccagt caatgatcga
    27241 taatcaacaa ctggtcagcc gggtaatccc aacgatgcgc cctccttcgc cattgagtct
    27301 ggcatggcgc aaatttggca gcggcaaagc ggtagaagat attgtgacct tgtttaccca
    27361 gcgcaggccg gaaatcagcg gatttttaga aattttcggc aacccacgca gttaaaaatc
    27421 aagtttcctt gcgcgttggc gcgtaaaata gcgcgcaagc tgatttgagg ttcactttta
    27481 actatgcaag agagacacac ggaacaggac tatcgtgccc tgctgattgc tgatacgccc
    27541 attattgatg ttcgcgcccc tatagagttt gagcaaggcg caatgcccgc cgctatcaat
    27601 ctgccgttaa tgaataacga tgaacgcgcc gccgttggca cctgctataa acagcaaggc
    27661 tcagacgcag cgctggcgct gggacataaa ctggtggcgg gtgaaattcg tcagcagcgc
    27721 atggacgcct ggcgggcagc gtgcctgcaa aatccgcaag gtattctctg ctgcgcccgt
    27781 ggcggtcagc gctcacatat tgtgcaacgc tggttgcatg cagcggggat tgattatccg
    27841 ctggtggcag gcggttataa ggcactgcgc cagaccgcga ttcaggcgac tattgaactg
    27901 gcacaaaaac cgatagtgct gattggcggt tgtaccggca gcggtaaaac gctgttagtg
    27961 cagcaacagc cgaacggtgt tgatctggaa gggttggcgc gtcatcgcgg ttcggcgttt
    28021 ggtcgcacgt tacaaccaca acttagccag gcgagttttg aaaacctgct ggctgccgaa
    28081 atgctaaaaa ccgacgcccg tcagaatttg cgcctgtggg tgctggaaga cgaaagccgg
    28141 atgatcggtt cgaatcacct gccggaatgc ctgcgcgagc gaatgactca ggcggcgatt
    28201 gcggtggtag aagatccgtt tgagatccgt cttgagcgcc tgaacgaaga gtatttcttg
    28261 cgtatgcatc atgattttac ccacgcgtac ggcgacgaac agggctggca ggaatattgc
    28321 gaatacctgc atcacggact ttcggctatt aagcgtcggc tggggctaca gcgatataac
    28381 gaactggctg caaggctgga tgcagcactg acaacgcaac tcaccaccgg cagcaccgac
    28441 ggtcatctgg cctggctggt gccgttactt gaagaatatt acgacccgat gtatcgctat
    28501 cagcttgaga aaaaagcgga aaaagttgtc tttcgcggtg agtgggcgga agtggcggaa
    28561 tgggttaagg cgcagtgatg agggacgtat ttgtattagt cgcgacttga tctgacacat
    28621 ggccttgaaa ggttgagagt taccggtttt gatatgagtg tcgaatcctt atccaaaaca
    28681 agaggtaact ctcatgcttc atactgccaa tcccgtcatc aaacacaaag ccggtttgct
    28741 caatctggct gaagaactca gcaacgtatc aaaagcctgt aaaatcatgg gcgtttcgcg
    28801 tgatacgttt tatcgttacc gcgaactggt cgctgaaggc ggtgtggatg cgcagattaa
    28861 tcgtagtcgc cgcgctccta accttaagaa ccgtaccgat gaggcaactg aacaggctgt
    28921 tgttgattac gccgtcgctt tcccgacaca tggtcagcac cgggccagca acgaactgcg
    28981 taaacagggg gtttttatct ccgacagtgg tgtccgttct gtctggttac tccataacct
    29041 tgagaatctc aaaagacgat actgaacaaa ttttatcagg tgacgttccg taaaaagttg
    29101 tatggagttc tcgatgcagt cggcgcgact atcagtaccc ccgctgaacc ctggactatc
    29161 gggccgccgt actggggcat gtcaccctgg cgtgccgctg gttttccgct aatggtttat
    29221 ttcctctttc tttcccggca agaatttatt ttaatccgga aggattattt attttcagat
    29281 agggactcac attaaacacg taacatttta attaatgcat ggctgaatat gtattacaca
    29341 atgtcagata tcgtaaaaga ccagtgcaat tctatgtaaa ctctctgact gttcatttta
    29401 tttgttgttt cagggtcgga actcaccctc aataaacaac tgaatacgag tagatattca
    29461 tcgtctgagc tatatggctt tacacaatag ccgacatgtc ttttctcagt caggaatttt
    29521 acttttcctc actacgaagt tgacgacttc gatatgggat agactcttaa ttcaagcaat
    29581 tactctgcac aagcatcagg caatccagct ccaatatttg ttataaaata atgcgtaaca
    29641 ttaatttcac caacaaaaaa aagatttacg ctatcccctt gccctggtat tttaccatat
    29701 ttacaccaca ctttttttgt atctggatca ttctttgata cgattttcgc cacttgttcc
    29761 ctggccctca ccgtcaatgt tttcttaaac ctccccccat catcgattgt tagggaaaaa
    29821 taagaaaaaa aataatcatc ttctacattt gtggatattg atatatccac cgctacgtgc
    29881 ggaaaaaaaa tctgggagcc atgtaaaaac tccatcattt tctggtattg ccatatagtt
    29941 tatgtaatgc ttacttacaa gagattcatc tttgtgtaaa taaagataag tagtcacgca
    30001 taaaaaaaca acgattataa tagcaaaaat aaatattacc atctttgaga tgttacttga
    30061 g
//
LOCUS       DAZGBC010000047        30057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGBC010000047 DAZGBC010000000
VERSION     DAZGBC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.047"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(189..1853)
                     /gene="glnS"
                     /locus_tag="ACHK1J_002070"
     CDS             complement(189..1853)
                     /gene="glnS"
                     /locus_tag="ACHK1J_002070"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO7911339.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2056..4002)
                     /gene="nagE"
                     /locus_tag="ACHK1J_002071"
     CDS             complement(2056..4002)
                     /gene="nagE"
                     /locus_tag="ACHK1J_002071"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO7911340.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATTAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4335..5135
                     /gene="nagB"
                     /locus_tag="ACHK1J_002072"
     CDS             4335..5135
                     /gene="nagB"
                     /locus_tag="ACHK1J_002072"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7911341.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5195..6343
                     /gene="nagA"
                     /locus_tag="ACHK1J_002073"
     CDS             5195..6343
                     /gene="nagA"
                     /locus_tag="ACHK1J_002073"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7911342.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6352..7572
                     /gene="nagC"
                     /locus_tag="ACHK1J_002074"
                     /gene_synonym="nagR"
     CDS             6352..7572
                     /gene="nagC"
                     /locus_tag="ACHK1J_002074"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO7911343.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7620..8372
                     /gene="nagD"
                     /locus_tag="ACHK1J_002075"
     CDS             7620..8372
                     /gene="nagD"
                     /locus_tag="ACHK1J_002075"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO7911344.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8423..8521)
                     /locus_tag="ACHK1J_002076"
                     /pseudo
     CDS             complement(8423..8521)
                     /locus_tag="ACHK1J_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8769..10433
                     /gene="asnB"
                     /locus_tag="ACHK1J_002077"
     CDS             8769..10433
                     /gene="asnB"
                     /locus_tag="ACHK1J_002077"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO7911345.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10813..10889
                     /locus_tag="ACHK1J_002078"
     tRNA            10813..10889
                     /locus_tag="ACHK1J_002078"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10847..10849,aa:Met,seq:cat)
     gene            10899..10983
                     /locus_tag="ACHK1J_002079"
     tRNA            10899..10983
                     /locus_tag="ACHK1J_002079"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10933..10935,aa:Leu,seq:tag)
     gene            11007..11081
                     /locus_tag="ACHK1J_002080"
     tRNA            11007..11081
                     /locus_tag="ACHK1J_002080"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11039..11041,aa:Gln,seq:ttg)
     gene            11116..11190
                     /locus_tag="ACHK1J_002081"
     tRNA            11116..11190
                     /locus_tag="ACHK1J_002081"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11148..11150,aa:Gln,seq:ttg)
     gene            11206..11282
                     /locus_tag="ACHK1J_002082"
     tRNA            11206..11282
                     /locus_tag="ACHK1J_002082"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11240..11242,aa:Met,seq:cat)
     gene            11331..11405
                     /locus_tag="ACHK1J_002083"
     tRNA            11331..11405
                     /locus_tag="ACHK1J_002083"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11363..11365,aa:Gln,seq:ctg)
     gene            11443..11517
                     /locus_tag="ACHK1J_002084"
     tRNA            11443..11517
                     /locus_tag="ACHK1J_002084"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11475..11477,aa:Gln,seq:ctg)
     gene            complement(11671..12846)
                     /gene="ubiF"
                     /locus_tag="ACHK1J_002085"
     CDS             complement(11671..12846)
                     /gene="ubiF"
                     /locus_tag="ACHK1J_002085"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO7911346.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRSKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12992..14416
                     /gene="miaB"
                     /locus_tag="ACHK1J_002086"
     CDS             12992..14416
                     /gene="miaB"
                     /locus_tag="ACHK1J_002086"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO7911347.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14569..15609
                     /gene="ybeZ"
                     /locus_tag="ACHK1J_002087"
     CDS             14569..15609
                     /gene="ybeZ"
                     /locus_tag="ACHK1J_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO7911348.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15606..16073
                     /gene="ybeY"
                     /locus_tag="ACHK1J_002088"
     CDS             15606..16073
                     /gene="ybeY"
                     /locus_tag="ACHK1J_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO7911349.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16163..17041
                     /gene="corC"
                     /locus_tag="ACHK1J_002089"
     CDS             16163..17041
                     /gene="corC"
                     /locus_tag="ACHK1J_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO7911350.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17066..18604
                     /gene="lnt"
                     /locus_tag="ACHK1J_002090"
     CDS             17066..18604
                     /gene="lnt"
                     /locus_tag="ACHK1J_002090"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO7911351.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWSKTTWLRVAIVAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSTLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            19001..19909
                     /gene="gltI"
                     /locus_tag="ACHK1J_002091"
     CDS             19001..19909
                     /gene="gltI"
                     /locus_tag="ACHK1J_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO7911352.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            20079..20819
                     /gene="gltJ"
                     /locus_tag="ACHK1J_002092"
     CDS             20079..20819
                     /gene="gltJ"
                     /locus_tag="ACHK1J_002092"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO7911353.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            20819..21493
                     /gene="gltK"
                     /locus_tag="ACHK1J_002093"
     CDS             20819..21493
                     /gene="gltK"
                     /locus_tag="ACHK1J_002093"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO7911354.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21493..22218
                     /gene="gltL"
                     /locus_tag="ACHK1J_002094"
     CDS             21493..22218
                     /gene="gltL"
                     /locus_tag="ACHK1J_002094"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO7911355.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22336..23271
                     /gene="rihA"
                     /locus_tag="ACHK1J_002095"
     CDS             22336..23271
                     /gene="rihA"
                     /locus_tag="ACHK1J_002095"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO7911356.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQKC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHGEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23355..25025
                     /gene="hscC"
                     /locus_tag="ACHK1J_002096"
     CDS             23355..25025
                     /gene="hscC"
                     /locus_tag="ACHK1J_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO7911357.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHTSAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIEIP"
     gene            complement(25085..26536)
                     /gene="djlC"
                     /locus_tag="ACHK1J_002097"
     CDS             complement(25085..26536)
                     /gene="djlC"
                     /locus_tag="ACHK1J_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO7911358.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDDATEHEILRVFRALLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSVGMDEEEREAFLEAI
                     QAGDCFDFLGLLEYPVALQNQTVEYYFALERCCHYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWGSYPSTQADNLLLQWSAQHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26533..27240)
                     /gene="ybeU"
                     /locus_tag="ACHK1J_002098"
     CDS             complement(26533..27240)
                     /gene="ybeU"
                     /locus_tag="ACHK1J_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7911359.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27342..27896
                     /gene="ybeT"
                     /locus_tag="ACHK1J_002099"
     CDS             27342..27896
                     /gene="ybeT"
                     /locus_tag="ACHK1J_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7911360.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27906..29333)
                     /gene="djlB"
                     /locus_tag="ACHK1J_002100"
     CDS             complement(27906..29333)
                     /gene="djlB"
                     /locus_tag="ACHK1J_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO7911361.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEECEVAEHEILLAFSALLASDSERFLPSAWQRFIQQLNY
                     CSMDEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENTGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGKSLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29330..30037)
                     /gene="ybeR"
                     /locus_tag="ACHK1J_002101"
     CDS             complement(29330..30037)
                     /gene="ybeR"
                     /locus_tag="ACHK1J_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7911362.1"
                     /translation="MDMKSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT         7501 a         7735 c         7545 g         7276 t
ORIGIN      
        1 gcatgtctct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag cacgcaaact
       61 caatatattg cagagatcat gtaggcctga taagcgtagc gcatcaggca atttagcgtt
      121 tgaaactgtt tgtctgttcc ttgccggatg cgacgtaaac gccccatccg gcatagcgaa
      181 acttaaaatt actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat
      241 accggttttt ccgccgtaga atggcggcta tcgaggcaga agtaaccttc acgctcaaac
      301 tggaatgctt tacccgctac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc
      361 accagcgatt ccgggttaat caccgacagg aaatcatccg cagcacctgg gttaggcacg
      421 ctgaacagac gatcatacaa acggatttca accggcagcg catgtgccgc gctcacccag
      481 tgaataacgc ctttgacttt acgaccatca gccggatctt tgcttaaggt gtcggcgtca
      541 taagtacaga agatggtggt gatattacct tcggcatctt tctcgacgcg ttctgcctta
      601 atcacataag cattacgcag acgcacttct ttaccgagca ccagacgttt gtactgcttg
      661 ttagcttctt cgcggaaatc ggcgcgatcg atccaaatct caccgctaaa cggcacctga
      721 cggctgccca tttccggttt gttcggatgg ttcggcatgg taaccatttc gccttcgccc
      781 tgatagtttt cgataaccag tttcaccgga tcgataaccg ccattgcgcg cggcgcattt
      841 tcgttgagat cttcacggat gcaggattcc agcgacgcca tctcaatggt gttgtcctgc
      901 ttggtcacgc cgatgcgttt gcagaactca cgaatagaag ccgcagtgta accacgacga
      961 cgcagaccgg aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttatcggtc
     1021 accagcaggt tcagcttacg cttggacatc acagtgtatt ccagattcag gcgcgagaac
     1081 tcatactggc gcgggtgaac aggaatggtg atgttgtcca gtacccagtc gtacagacga
     1141 cggttgtcct ggaactccag cgtacacaga gagtgcgtaa taccttccag cgcatcgctg
     1201 atgcaatggg tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt
     1261 tcagcaaatt taatgcggta cagcaccgga tcgcgcatca cgataaacgg tgaagccatg
     1321 tcgattttcg cacgcaggca ggctttacct tcttcaaaac cgccggtacg cattttctcg
     1381 aacagcgcca ggttctcttc aacgctacgg tcgcggtacg ggctgttttt accaggctgc
     1441 gtcagggtgc cgcggtattc gcggatctgt tccggcgtca gttcatcaac gtacgccagg
     1501 cctttattga tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa
     1561 cggacgttac cagaccagtg aaaacctaac cactcaacgt cgtttttgat cgaatcgaca
     1621 tactcgatat cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta
     1681 tagtcctggg cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag
     1741 ccattcggct ccggcgggaa acgggtgtgt actgtggtgt gcttaccact ggccagatct
     1801 tcatcgatga tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat
     1861 tcctcaaagc gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa
     1921 ctgttagtta gtgaaaatcg aaaatctcac tgagattatg gggatgattg atgaaaaaaa
     1981 aagcggcaga gaagatctcc accgcttgtc attgttggat gcgacgctca agcgtcgcat
     2041 caggcataaa gcagattact ttttgatttc atacagcggt gtttgacccg ccacaacatg
     2101 gccctgagct ttaatgatca agccactgaa atcgtcgata ttactgcaaa ccactgggct
     2161 aatcatcgag cgggcgttag cgttcaggta atccagatcc atttccagaa tcggttgtcc
     2221 tgcgcttacc tgcgcgccct cttccaccag acgtttaaag cctttacctt ccagcgctac
     2281 ggtgtcgata cccatatgga cgacgatctc cgcgcctttt tcggtttcca gacagaacgc
     2341 gtggttggtg ttgaagattt tcacgattgt ccctgcggct ggtgatacga cgattttatc
     2401 tgtcggtttc accgccacac cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac
     2461 ctgatccagt gccacgacat caccggtaat cggcgatacc agctccgcga tagataccgc
     2521 gtttggtaca gcctgcggtt ttgctacagg agcggtagtt gccggagcag tttcagcaga
     2581 ggcagctgct accggaccgc gggcaacgac tttcttcatc gcatcgccga tggattctgc
     2641 tttcgcgcca acaatcacct gaatagtctg tttgttcagt tttactaccc cagaagcccc
     2701 cagacgttta cacatcgtat cgttaacgcg ggcagagtca gccacggtaa ggcgcagacg
     2761 ggtgatacag gcgtcaattg ctttcaggtt gtcagtgccg ccaaccgcag caatatagtt
     2821 ggtcgccagt tgattgaggc cttcttcagt gttgctgttg gcttcttcag taacgatctc
     2881 gtcttcttta tcttcacgac ccggcgtttt caggttgaac atgcggataa ccaaactgaa
     2941 caccacgaag tagatagcga agaagacaac acccataacc agcagcatcc agacgttctg
     3001 gctggcggcc ggcaggttat acatcaacgc gtagtcgata gcccccgcag agaaagagaa
     3061 gccggcatgg atacccagca gcgttgccac aaacaggctg ataccggtca gcagtgcgtg
     3121 caggaggtac agcagcggag caaggaacat gaacaggaat tccagcggct cagtcacacc
     3181 ggtcaggaac gcagtaacag caacagaaag cagcatcccg ccaaccatcg gacgacgctc
     3241 tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc aggccgaaca tcatgatcgg
     3301 gaagaagccg gacatgaaca tccccgcggt gccgtcacca gcgtagaaac ggttgatgtc
     3361 gccgtggaaa accgtacccg ccgcgttggt gaattcacca atctggaacc aggcgatggt
     3421 gttcagcacc tgatgcagac cggttgggat cagcagacgg ttgatgaaac caaagatacc
     3481 ggaacccagc gcgcccgcag aaacgatcca ctcgccgcct gcatggatag cgtgctgtac
     3541 cggcggccag acgtaaccaa aaatggccgc cagcaccaga cagaagaagc cggtggcgat
     3601 cggcacaaag cgtttgccgc cgaagaagct caggaagtcc ggcagtttaa tatcggacca
     3661 acggttatag gctgcgccac caaccagacc ggtaatgata cccgccagta cacccatgtt
     3721 aatttctggg ttgatggtca ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc
     3781 cagcgccgcc gcacctgcgc tgtctttcga ccagctggat gccacaccga tggcgaagat
     3841 taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa
     3901 atctggctga ccgaatcgca gcaacagtgc cgccaccggc agcaccgcga tagggagctg
     3961 taacgcccta ccgagtcgct ggaaaaaacc taaaatattc atcttattcc ccctacgaga
     4021 accctatttg gctcgtttaa agccgtattt ttattgtgct gcaaattgta ctgccgatgt
     4081 tctgtaatca gattgttaga tcatctgcca cagagtgtgt gaaaatttaa ttcgtatcgc
     4141 aaattaaacg cgtgtctttt gtgagttttg tcaccaaata tcgttatcat cactcccttt
     4201 tactggctaa accagaaaac ttattttatc attcaaaaaa tcaggtcgga ttgacgcctg
     4261 tctgcgcaaa tccaggttac gcttaaagat gcctaatccg ccaacggctt acattttact
     4321 tattgaggtg aataatgaga ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg
     4381 ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc gactgctgat cgtccgtttg
     4441 ttctgggcct gccaactggc ggcacgccaa tgaccaccta taaagcgtta gtcgaaatgc
     4501 ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt caacatggac gagtatgtcg
     4561 gtctgccgaa agagcatccg gaaagctact acagctttat gcaccgtaat ttcttcgatc
     4621 acgttgatat tccagcagaa aacatcaacc ttctcaacgg caacgccccg gatatcgacg
     4681 ccgagtgccg ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg
     4741 gcggtgtagg taacgacggt catattgcat ttaacgaacc ggcgtcttct ctggcttctc
     4801 gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc gaactctcgt ttctttgata
     4861 acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca ctgctggatg
     4921 ccgaagaagt gatgattctg gtgctgggta gccagaaagc actggcgctg caggccgcag
     4981 ttgaaggttg cgtgaaccat atgtggacca tcagctgtct gcaactgcat ccgaaagcca
     5041 tcatggtgtg cgatgagcct tccaccatgg agctgaaagt taagacttta agatatttca
     5101 atgaattaga agcagaaaat atcaaaggtc tgtaattgtt atccctgccc tgacaccttg
     5161 ctcagggcaa tattttttaa aatcgggggt cagaatgtat gcattaaccc agggccggat
     5221 ctttaccggc cacgaatttc ttgatgacca cgcggttgtt atcgctgatg gcctgattaa
     5281 aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa caacgttcac tgaacggggc
     5341 cattctctcc cccggtttta tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa
     5401 cgacaccgct gaagcggtca gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa
     5461 atcaggctgt actaactatc tgccgacgct tatcaccacc agcgatgagc tgatgaaaca
     5521 gggcgtgcgc gttatgcgcg agtacctggc aaaacatccg aatcaggcgt taggtctgca
     5581 tctggaaggc ccgtggctga atctggtgaa aaaaggtacc cataatccga attttgtgcg
     5641 taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt
     5701 gaccctggca ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa atgccgggat
     5761 tgtggtttct gccggtcact ccaacgcgac gttgaaagaa gcgaaagccg gtttccgcgc
     5821 ggggattacc tttgccaccc atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc
     5881 gggcctggcg ggcgcgatcc tcgatgaagc tgacatttat tgcggtatta tcgctgatgg
     5941 cctgcatgtt gattacgcca acattcgtaa cgctaaacgc cttaaaggcg acaaactgtg
     6001 tctggttacc gacgccaccg cgccagcagg tgccaacatt gaacagttca tttttgcggg
     6061 taaaacaata tactaccgta acggactttg tgtggatgag aacggtacgt taagcggttc
     6121 atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga
     6181 tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg attggcgttg agaaacgtct
     6241 cggcacactc gccgcaggta aagtagccaa cctgaccgca ttcacacctg attttaaaat
     6301 caccaagacc atcgttaacg gtaacgaggt cgtaactcaa taagagaaag tatgacacca
     6361 ggcggacaag ctcagatagg taatgttgat ctcgtaaaac agcttaacag cgcggcggtt
     6421 tatcgcctga ttgaccagta cgggccaatc tcgcggattc agattgccga gcaaagccag
     6481 cttgcccccg ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa
     6541 gaagttgatc agcaggcctc caccgggggc cgccgcgcta tctctatcgt caccgaaacc
     6601 cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcacg acgccaccat caccctgttt
     6661 gatctcagca gcaaagtgct ggcagaagaa cattacccgc tgccggaacg tacccagcag
     6721 acgctggaac atgccctgct gaatgccatt gctcagttta ttgatagcta ccagcgcaaa
     6781 ctgcgcgagc tgatcgccat ttccgttatc ctgccaggac ttgttgaccc ggacagcggc
     6841 aaaattcatt acatgccgca tattcaggta gaaaactggg ggctggtaga agcactggaa
     6901 gagcgtttta aagtgacctg tttcgttggt cacgatatcc gtagtctggc gctggcggag
     6961 cactacttcg gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga
     7021 accggggccg ggattatctc taacgggcgc atttttattg gccgcaacgg taacgtcggt
     7081 gaaattggcc atattcaggt cgaaccgctg ggtgaacgct gccactgcgg caactttggc
     7141 tgcctggaaa ctatcgctgc caacgccgct attgaacaac gggtgttgaa tctgttaaag
     7201 cagggctacc agagccgcgt gccgctggac gactgcacca taaaaactat ctgcaaagct
     7261 gcgaacaaag gcgatagcct ggcgtcggaa gtgattgagt atgtcggtcg tcatctgggc
     7321 aaaaccatcg ccattgccat caacctgttt aacccgcaaa aaattgttat tgccggtgaa
     7381 atcaccgaag ccgataaagt gctgctccct gctattgaaa gctgcattaa tactcaggcg
     7441 ctgaaggcgt ttcgcactaa tctgccggtg gtacgttctg agctagacca ccgctcggca
     7501 atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct ccagcatttg
     7561 ctggaaaatt aatgtgcttt tatagtggcg cttattgttg tcaatattct gggtagtcca
     7621 tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg
     7681 taccgggtgc agcggaattt ttgcacggga ttatggataa aggcctgccg ctggtgttgc
     7741 tgaccaacta tccttcgcag accgggcaag atctggcgaa ccgctttgcc actgccggtg
     7801 tcgatgtacc tgacagcgtg ttttatacct ctgcgatggc gaccgccgat ttcctgcgtc
     7861 gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc actgattcat gaactgtaca
     7921 aagccggttt cactattacc gatgtgaacc ctgattttgt cattgttggc gaaacgcgtt
     7981 cctacaactg ggacatgatg cataaagcgg cctatttcgt cgctaacggt gcacgcttta
     8041 tcgccaccaa cccggatact cacgggcgcg gtttttatcc ggcctgtggc gcgttgtgtg
     8101 cagggattga gaaaatctcc gggcgcaaac cgttctatgt tggtaagccg agtccgtgga
     8161 ttatccgcgc ggcattaaac aaaatgcagg cacattcgga agaaacggtg attgtcggcg
     8221 ataacctgcg taccgatatt ctggccggct tccaggcagg tctggagacg attctggtgc
     8281 tttctggtgt ttcgtcgctc gacgatatcg acagtatgcc gttccgcccc agctggattt
     8341 acccatcggt cgctgaaatc gacgttatct gaatagcaac cacgtctgcg gacgtggttg
     8401 gctctgatga caaacgccaa actgcctgat gcgctacgct tatcaggcct acgtagctcc
     8461 tgcaacatat tgaattttca tgcttttgta ggccggataa ggcgttcacg ccgcatccgg
     8521 catgaacaaa tcgcactttg tcagcaattt ggcccccgta aaatataaac attcaataaa
     8581 acccgccaaa aattatcgat ccattaataa atacatcacc gcaccctccc ctttttcaca
     8641 aatcagcaat caccattacg ttttttattt tttcaccgcc aaatcgcttg cgcacagtgc
     8701 tgctttgcgg cattttttta aacaagcaaa cacaacaagc aacaaatacc aggttaacgg
     8761 agaaggttat gtgttcaatt tttggcgtat tcgatatcaa aacagacgca gttgagctgc
     8821 gtaagaaagc actcgagctg tcacgcctga tgcgtcatcg tggcccggac tggtccggta
     8881 tttatgccag tgataacgcc attctcgccc acgaacgtct gtcaattgtt gacgttaacg
     8941 cgggggcgca acctctctac aaccaacaaa aaacccacgt actggcggta aacggtgaaa
     9001 tctacaatca ccaggcattg cgcgctgaat acggcgatcg ttatcagttc cagacagggt
     9061 ctgactgtga agtgatcctc gcgctgtatc aggaaaaagg gccggaattt ctcgacgact
     9121 tgcagggcat gtttgccttt gccctgtacg acagcgaaaa agatgcctac ctgattggtc
     9181 gcgaccatct ggggatcatc ccactgtata tgggctatga cgaacatggt cagctgtatg
     9241 tggcctcaga aatgaaagcc ctcgtgccgg tatgccgcac gattaaagag ttcccggcgg
     9301 ggagctattt gtggagtcag gacggcgaaa tccgttctta ttatcatcgc gactggttcg
     9361 actacgatgc ggtgaaagat aatgtcaccg acaaaaacga gctgcgtcag gctctggaag
     9421 attccgttaa aagccatctg atgtctgatg tgccttacgg tgtgctgctt tctggtggtc
     9481 tggattcctc aattatttcc gctatcacca agaaatacgc agcccgtcgc gtggaagatc
     9541 aggaacgctc tgaagcctgg tggccgcagt tacactcctt tgctgtaggt ctgccgggtt
     9601 caccggatct gaaagcagcc caggaagtgg caaaccatct gggcactgtg catcatgaaa
     9661 ttcacttcac tgtacaggaa ggtctggacg ccatccgcga cgtgatttac cacatcgaaa
     9721 cctatgatgt gaccactatt cgcgcttcaa caccgatgta tttaatgtcg cgtaagatca
     9781 aagcgatggg cattaaaatg gtgctgtccg gtgaaggttc tgatgaagtg tttggcggtt
     9841 atctttactt ccacaaagca ccgaatgcca aagaactgca tgaagagacg gtgcgtaaac
     9901 tgctggccct gcatatgtat gactgcgcgc gtgccaacaa agcgatgtca gcctggggcg
     9961 tggaagcacg cgttccgttc ctcgacaaga aattcctcga cgtggcgatg cgcattaacc
    10021 cgcaggataa aatgtgcggt aacggcaaga tggaaaaaca catcctgcgt gaatgttttg
    10081 agtcatacct gcccgcaagc gtggcctggc ggcagaaaga gcagttctcc gatggcgtcg
    10141 gttacagttg gatcgacacc ctgaaagaag tggcggcgca gcaggtttct gatcaacagc
    10201 tggaaactgc ccgcttccgc ttcccgtaca acacaccgac ctcaaaagaa gcgtatctgt
    10261 accgggaaat ctttgaagaa ctgttcccgc ttccaagcgc cgctgagtgc gtgccgggcg
    10321 gtccttccgt cgcgtgttct tcagctaaag cgatcgaatg ggatgaagcg ttcaaaaaaa
    10381 tggacgatcc gtctggtcgc gcggttggtg ttcaccagtc ggcgtataag taagaaatgc
    10441 gaaacagccc cggtgaaatc cggggctgtt tcagttattg aggtgcttgt tgattctgat
    10501 atgcagtaag gaaatgttcc gggtttgcca catcactgtt tgacaactca tcactgtagg
    10561 ccagtatttc atagattaat ttcccaccac tattgttaaa ttccgaattt catcctgtat
    10621 gacaaaagaa tggctgtagg gtgggaaaaa acacaaagta aacaataatt gacgaatata
    10681 gcgccacgct gttcgcaacc taaccaaaca gtcactttcg agcaattttc cttgaaaaag
    10741 aggttgacgc tgcaaggctc tatacgcata atgcgccccg caacgccgat aaggtatcgc
    10801 gaaaaaaaag atggctacgt agctcagttg gttagagcac atcactcata atgatggggt
    10861 cacaggttcg aatcccgtcg tagccaccat ctttttttgc gggagtggcg aaattggtag
    10921 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    10981 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    11041 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    11101 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    11161 tccctggttc gaatccaggt accccagcca tcgaagaaac aatctggcta cgtagctcag
    11221 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    11281 caaattctga atgtgtcgaa tatgttcgac aaattcaaaa accaatttgt tggggtatcg
    11341 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    11401 agccaattta ttcaagacgc ttaccttgta agtgcaccca gttggggtat cgccaagcgg
    11461 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    11521 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcactcaatg tcgaatgcga
    11581 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    11641 ggttggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    11701 acgtttcaac acgccagcac gctccgccgc catcaaacca agattacgca caaaacgcag
    11761 cggcggcaga ttattactga atccggcata aaacagatcc ataccgctct gcataatgaa
    11821 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    11881 gccgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    11941 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    12001 ccctggctgc acatactgca acgcatggcg acgcgtcagc ggaaacgcac cagcggcaag
    12061 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagctgtgc
    12121 catattcata ttctgcaact ggcgaatacg cgccggagag tcataccaca ccagcgatgc
    12181 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggagtaaatt gctgccaggt
    12241 gctatcgccg ggatcgttct cgcactggac gctaatcaac atgcacgact gcgcatactg
    12301 ccatgcatga acgccaattc ccgccatctg ccgcacctgc gaatttgcgc cgtcggcacc
    12361 aatcaccagc ttcgagcgaa tcacttcacc gcctttcagc tccagctcct gaagatcatt
    12421 atctcgatgt aatgcaatca gcgagcctgg cacacgtaac gttactttcg gatgcgcttc
    12481 cagcgcctgc cacaacgcct gttgcaggac agtgttttcc accatatagc caagcagcgg
    12541 tagcttaagt tcagcggcgt caaacaccac atgcgccgtt tcccactccc acgtttccag
    12601 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    12661 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tatcagcgac
    12721 aaacggtgca ggttctgcgt gctcgatcac cgttaccgta aatccgtgct gtgccagccc
    12781 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    12841 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    12901 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    12961 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    13021 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    13081 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctgctgctga acacctgctc
    13141 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    13201 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    13261 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    13321 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    13381 cccggaaatc gagaagtttg accgtctacc ggaaccgcgc gccgaagggc cgaccgcgtt
    13441 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    13501 tggtgaagag gtaagccgtc cgtccgacga tattctgttt gagattgccc agcttgcggc
    13561 tcagggcgtg cgtgaagtca acctgctcgg tcagaacgtg aacgcctggc gtggtgaaaa
    13621 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    13681 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    13741 agtgtatcgc gacacgccgg agctggtgag cttcctgcat ctgccggtac agagcggttc
    13801 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    13861 ccgtaaactg cgtgcggcgc gtccagatat tcagatcagt tctgatttca tcgttggctt
    13921 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    13981 cgacatgagc tacagcttta tcttctctgc acgtccgggt acaccagccg ccgatatggt
    14041 tgatgatgtc ccggaagaag agaagaagca gcgtctgtat attctgcaag agcgcattaa
    14101 tcagcaagcg atggcgtgga gccgccggat gctcggcacc acccagcgta ttctggtaga
    14161 aggtacatcg cgtaagagca tcatggagct ttccgggcgt acggaaaata accgcgtggt
    14221 caacttcgaa ggcacgccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    14281 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcgt
    14341 ggcagaaaca ccggaatcgg tgatagcccg tactcgcaaa gaaaacgacc ttggcgtggg
    14401 ttattatcag ccgtaattct caggcccgcc gttccggtgg gccttgtttt ttctctcttt
    14461 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    14521 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    14581 actcgcgaaa tcaccctgga gccagcagac aacgcgcgtc tgttgagcct gtgcggcccg
    14641 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    14701 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    14761 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatattga accggaacag
    14821 atccaccttg cgattaaaga agcgcgggta ttggagcaaa gtgcggagag cgtgccggag
    14881 tacggcaaag cggtcaatat caaaaccaaa cgcggcgtaa ttaagccgcg cacgccaaac
    14941 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    15001 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    15061 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    15121 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    15181 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    15241 ccgctggcct atatgcgtgg tcgtacgctg aatgacgcct ttatcattct cgatgagagc
    15301 cagaacacca ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    15361 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    15421 cgtcatgcca tcgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    15481 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcctatga agcctgggaa
    15541 gaagccgaac aaaaacgtaa agcggcgctg gcggcagaac gcaagcgcga agaacaggaa
    15601 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataac tccggattgc
    15661 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    15721 cggaagtgac gattcgcgtg gtcgataccg ccgaaagcca cagtctcaat ctgacctatc
    15781 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    15841 aaatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    15901 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    15961 tgttaggtta cgatcacatc gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    16021 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    16081 gccatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    16141 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    16201 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    16261 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    16321 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgtcc gcgacatcat
    16381 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    16441 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    16501 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    16561 cagcatggac aaagtgttac gtcaggcagt tgtcgttcct gaaagtaagc gcgtagaccg
    16621 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    16681 tggggtttcc ggtctggtaa ccattgaaga catcctggaa ctgattgttg gtgagattga
    16741 agacgaatat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    16801 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    16861 tgaagaagtc gacactatcg gtggtctggt gatgcaggca tttgggcatc ttccggcacg
    16921 tggcgaaacc atcgacatcg acggttacca gttcaaagtg gcgatggccg acagtcggcg
    16981 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccgaagc tggatgaata
    17041 aaaccgaaac tggatagata actacatggc ttttgcctca ttaattgaac gccagcgcat
    17101 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    17161 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgctgacct ttaaccgccg
    17221 tccactccag tctgccgcta ttggcttttg ctggggattt ggcctgtttg gcagcggtat
    17281 taactgggtc tatgtcagta tcgcgacctt tggcggaatg cctggcccgg ttaacatctt
    17341 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgtt
    17401 gtcgcgtctg tggtcgaaaa ccacctggct gcgcgtggcg attgtcgctc ctgctctctg
    17461 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    17521 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    17581 taacttcctg cttatgatgg ttagcggcct gctggcactg gcgttagtca aacgcaactg
    17641 gcgtccgctg gtggtggccg tcgtgctgtt tgcccttccc ttcccgctgc gttacatcca
    17701 gtggtttacc ccacaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    17761 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctgaagattt actacaacgc
    17821 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    17881 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    17941 gagtacgctg gtgaccggga ttgttgacgc gcgtcttaat aagcagaacc gttacgatac
    18001 ctacaacacc atcatcacac tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    18061 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    18121 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    18181 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agatcattct
    18241 cggcgagcaa gtgcgcgata acttccgccc ggataccgac tatctgctga ctatctccaa
    18301 cgatgcgtgg tttggtaagt ctattggtcc atggcagcac ttccagatgg cgcgaatgcg
    18361 tgcgctggag ctggcgcgcc cattgctgcg tagtaccaac aacggcatta cggcggtgat
    18421 tggtccgcag ggtgagattc aggcgatgat ccctcagttc acccgcgaag tgttgaccac
    18481 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    18541 ggtgctgacg gcactgtttg gttttgccgc tgtgctgatg agtctgcgtc aacgacgtaa
    18601 ataatccctt cttatcgtgc cggaatacat cccgtatttc ggcacgctca ccggattcgg
    18661 catcagtgcg ttatccggat aacaaaattc ccctatccaa ctttggcaca tctattgctt
    18721 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    18781 accggtagca ccaaaagagt gcattttaag aattttgctt cggaatggtg cggcgcacct
    18841 cccaaatata aacaataaaa cagcaatatc tttacattta gccaaactaa atgttaccta
    18901 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    18961 tatccatgcg ttcttaacgc agaagataaa ggagttggat atgcaattac gtaaacctgc
    19021 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    19081 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    19141 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    19201 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    19261 ggtgaaactg attccgatca cctcacaaaa ccgtattcca ctgctgcaaa acggcacttt
    19321 cgattttgaa tgtggttcca ccaccaataa cgtcgaacgc caaaaacagg cggctttctc
    19381 tgacactatt ttcgtggtcg gtacgcgcct gctgaccaaa aagggcggcg atatcaaaga
    19441 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    19501 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    19561 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgatgc
    19621 cctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    19681 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    19741 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    19801 taaatggttc aaaaatccca ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    19861 cgaaatgaaa gcactgttca aagaaccgaa tgacaaagca ctgaactaat tacaagaacc
    19921 aggggcggaa attctagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    19981 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    20041 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    20101 gtattttttt acaacaagcc ccgttcggca acaccaccta tcttggttgg atctggagtg
    20161 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    20221 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    20281 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    20341 tcccggagtt gctgccagag aaaatcggca tgtggtttaa ggccgagctg gacccaaata
    20401 ttcagttttt cctctcctcc atgctctgtc tggggctgtt taccgccgcc cgtgtttgcg
    20461 aacaggtacg cgccgccatt cagtcgctgc cgcgcgggca aaagaatgcc gcgctggcga
    20521 tggggctgac gctgccgcag gcttaccgct atgtactgtt gcctaatgct tatcgcgtta
    20581 tcgtcccgcc gatgacctcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    20641 ctatcggtct ggtagatatg gcagcgcagg cgggtaaatt gctcgattac tcggcccacg
    20701 cctgggagtc gtttaccgcc attacgctgg cgtatgtgtt gattaacgcg gttatcatgc
    20761 tggtaatgac gctggttgaa cgtaaagttc gcctgcctgg caacatgggg ggcaaataat
    20821 gtacgagttt gactggagtt ccattgtccc ttccctgcca tatctgctcg acgggctggt
    20881 gatcacgctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gcaccatgct
    20941 ggcggtgatg cgcttgtcca gctttgcgcc cgtagcgtgg tttgccaaag cctacgttaa
    21001 cgtattccgc tcgattcctt tagttatggt tttgctgtgg ttttacctga tcgtgccggg
    21061 ttttctgcaa aacgtgctgg gattatcgcc aaaaaatgat atccgtttaa tttcggcgat
    21121 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ctggtattca
    21181 aagtatttct cgtggtcagt ctagcgccgc gctggcgttg ggaatgactc actggcagtc
    21241 gatgaaactg attattctgc cgcaggcgtt ccgcgcgatg gtgccgctgc tgctcactca
    21301 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    21361 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gttgagatga tcctgtttgc
    21421 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    21481 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    21541 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    21601 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccggt gcagcaaggt
    21661 gaaatcaccg tcgatggtat cgtggttaac gataagaaaa ccgatctggc aaagctgcgt
    21721 tctcgcgtcg ggatggtatt ccaacatttc gagctgttcc ctcatctgtc gattatcgaa
    21781 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcaccggc tcgtgaaaaa
    21841 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    21901 ctttccggcg gccagcagca gcgtgtggca attgctcgcg cattgtgtat ggatcctatt
    21961 gcgatgctgt tcgacgaacc cacatcggca ctagacccgg agatgatcaa cgaagtactg
    22021 gacgtgatgg tggaactggc gaacgagggg atgaccatga tggtggtgac ccacgaaatg
    22081 ggctttgccc gtaaagtggc gaatcgggtg atctttatgg atgagggtaa aattgttgaa
    22141 gactcgccga aagatgcgtt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    22201 gcgaaaatcc tgcattaatc actctggcgc gcatacccaa gtgcgcgcca tctcacgctt
    22261 tatcgtctcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    22321 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    22381 gctatcgcaa tagttctcgc cctcgcctca ccagagcttg atgtcaaagc aattacgtct
    22441 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    22501 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    22561 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    22621 acgtttgcac cgcaaaagtg tacggcggtg gagctgatgg cgaaaacgct acgtgaaagt
    22681 gcggaacctg tcaccattgt ttctaccgga ccgcaaacta acgttgcttt gctgctcaat
    22741 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    22801 ctgggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccaga agcggcagaa
    22861 attgtattcc agtcagggat cccggtggtg atggccggtc tggatgttac tcacaaagca
    22921 caaattcacg gtgaggacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    22981 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    23041 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    23101 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg gatgacggtt
    23161 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaatggt cgatgttgat
    23221 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acacacgtca
    23281 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tgtttatctc tttttctttc
    23341 aagatataaa tgcaatggat aatgcagaac tcgccattgg tatcgatctc ggtactacca
    23401 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    23461 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    23521 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    23581 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    23641 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    23701 atgtggtgat ctctgttccg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    23761 cagcggagtt agccggttta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    23821 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    23881 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacacctccg
    23941 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gctggtcgat gaggttttaa
    24001 aacgcgcgga tgtcgccagg accacgctta acgagagtga actggcagcc ttgtatgcct
    24061 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg cagtaccagg
    24121 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    24181 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcacgtctg aagccgagtc
    24241 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    24301 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    24361 tgggcgcagc aattcaggct gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    24421 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgtcagggc gtttccggca
    24481 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    24541 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    24601 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    24661 atcagtcgat tgatattcgc tttagttatg atatcaacgg tttgcttgaa gttgacgtgc
    24721 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    24781 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    24841 tgctcatcaa tcgtaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    24901 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    24961 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagatcc
    25021 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    25081 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    25141 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    25201 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    25261 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    25321 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    25381 cccgatacaa atccagtaac ggtgaatctt gctctgtctc gctaccgact ctcgatttcc
    25441 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    25501 atagttttcc gctatgtaaa atttccgccc ataacagacg cgtccgggcg ctctcaccag
    25561 gaacatattt caacggttta ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    25621 ctaataacgc gaaataatcc gggcaatgtt gtgcgctcca ctgcaacaac aaattatcgg
    25681 cttgtgtaga tgggtaactt ccccagtacg cgcagagatc ggcaagcagg ctttccccct
    25741 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    25801 ccatttgcca ctgtttggct acaggaatga gttccgccat acctgtttgc accgagctgt
    25861 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggaccttcca
    25921 tcgccaaaaa agcagtgaca tagtcaggat ggtaatggca gcaacgttcc agcgcgaagt
    25981 aatattcaac agtctggttc tgcaacgcaa cgggatattc cagaaggcca aggaaatcga
    26041 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattccta
    26101 cgctgtcatt aaacgaatgc cagttcaaac gctctgccag caaagaagca caatttaatg
    26161 aaagatatcg cgcttctata gcgatggcac acagcggcca gcgtaattga tcgacatcct
    26221 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    26281 cactttctga atccagtaat gccctgaata cacgtagtat ttcatgctca gtggcatcat
    26341 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    26401 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacat aacggtaagc
    26461 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    26521 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    26581 agttaatggc tgccagggaa gcgtggcata gaaacgatcg cttcccgctg ccagttcttc
    26641 gaacatacga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagt
    26701 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    26761 gtgcatccag ctgtgatagt aatggtgagc tcgtacatag acgcgcgatt gcagccataa
    26821 actctcgtct tccgtcagcc acttgttgag cacaccgacc cgtgacagaa accccattcg
    26881 aacataatcc caagctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    26941 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    27001 ggaagagcga tgccatgtca gatataagcc agctaaatca ttagcatgac catcatcagt
    27061 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    27121 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaaccctg
    27181 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    27241 ttgttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    27301 ttgacgcttt caaaataaaa agaaaatcga agcatccaca catgaataaa aaattaatgt
    27361 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    27421 cgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagctca ccgggcagtc
    27481 ttccattcac cacggcagcc acggagcttt ccgcgattca tggtcactca acgtcgcaat
    27541 atcgccttgg agaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    27601 cgagatactg gtatgagcaa tcagcggaac aggaaaatcc acgcgcgcaa agtaaactgg
    27661 ggtggatcta cctcaaaggt ctgggggtca aacccgacac ccgtaaagca attctctggt
    27721 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttaggt ttgatctaca
    27781 gaaatggctc aggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    27841 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg gcttcccgca cattaacgtc
    27901 gactgtcacc cgaacagatg gagtattttc cctatcaaac cagcaaagat aaaaatatag
    27961 aaaataattt taatgatgcc cagacagcct ggcccaccat cgtcaacggg cattggctgc
    28021 ggttgcggct ccggttcaac agtttgcttc tcggtaaaat tcgccgcctg aattaataat
    28081 cgacgaataa ttaatgcttc cagtggacaa cgattttctg catcaagacg gcttacgatc
    28141 ggcattaatg attcttctgt tggttggctg gcctgcacta attgtttggc aactaaatat
    28201 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    28261 tgcaaaacca tctcgaccag agaacgcccc agcgcagaga gtttttcatc actgagtatt
    28321 tcgtataaat gaaaacgcgt acgagcgctg tcaccaggga cataaagtag cggttttccc
    28381 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatcgacc
    28441 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atattcgcgc
    28501 cagtagtcac aaagttctgc cagcaggctt ttgccttctc cacaatagac gcgttgcgcg
    28561 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    28621 tccaggagtt cgggaattcc caggcgacct gcgctatacc agcggagtaa atttctgtgc
    28681 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    28741 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    28801 acggcaacgg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    28861 tataaaaagg attccagttc ttcttcgtct atttccccgg tattttcctc ctgtaaccac
    28921 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggct
    28981 attgtgcaca gcgaccagcg taattcatca atctcatcca tcgagcaata atttaattgc
    29041 tgaatgaatc gctgccaggc ggagggcaga aaacgttcac tatcagaggc aagtaacgca
    29101 ctaaaggcga gcagaatttc atgttctgct acctcacatt cttctggttg ccaaacagat
    29161 ttagcaggcg actgcgcaat ccggagcgct tcctcatacg cctgacgaag ttgtttaaaa
    29221 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    29281 atatcgacgt cagtcgtttc ctctatgttg aggatcttcc agcaattttt cataggtcgc
    29341 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc cagggtaagg
    29401 tcgcgtaata tgcatcgtca ctggcaaata attgataaaa catatcattg ccggaatcgt
    29461 cgtattcttt acgcgctaag aattcacgta ataaaggcag attatcatct tcgggagact
    29521 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    29581 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    29641 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    29701 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    29761 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    29821 aaaaccctgc caaccgagcg gcatgaccgt tatcgatcat tcggtgaatt gtgcgcagca
    29881 ggtcatcacg gtcatcaatt ccccaggagg aagaaaggtc aaaaaagcat gtacctaaat
    29941 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    30001 aaagtgcaaa caatattttt tgcgacttca tgtccatata atttatcctt aaacatt
//
LOCUS       DAZGBC010000048        29894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGBC010000048 DAZGBC010000000
VERSION     DAZGBC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.048"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            110..2488
                     /gene="ppsA"
                     /locus_tag="ACHK1J_002102"
     CDS             110..2488
                     /gene="ppsA"
                     /locus_tag="ACHK1J_002102"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO7911363.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVEQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2545..4191)
                     /gene="fadK"
                     /locus_tag="ACHK1J_002103"
     CDS             complement(2545..4191)
                     /gene="fadK"
                     /locus_tag="ACHK1J_002103"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO7911364.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWQEAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4248..4541)
                     /locus_tag="ACHK1J_002104"
     CDS             complement(4248..4541)
                     /locus_tag="ACHK1J_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7911365.1"
                     /translation="MSQNATVNVDVKLGVNKFHVDEGHPHIILAANPDIKEFQKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4538..5827)
                     /locus_tag="ACHK1J_002105"
     CDS             complement(4538..5827)
                     /locus_tag="ACHK1J_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7911366.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIHPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMHHFRKIPALMENPRLFSQYPRMIADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5883..6821)
                     /locus_tag="ACHK1J_002106"
     CDS             complement(5883..6821)
                     /locus_tag="ACHK1J_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO7911367.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQVQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6841..7605)
                     /locus_tag="ACHK1J_002107"
     CDS             complement(6841..7605)
                     /locus_tag="ACHK1J_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO7911368.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DNVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7921..8832
                     /locus_tag="ACHK1J_002108"
     CDS             7921..8832
                     /locus_tag="ACHK1J_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7911369.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWLLTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(8875..10026)
                     /locus_tag="ACHK1J_002109"
     CDS             complement(8875..10026)
                     /locus_tag="ACHK1J_002109"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO7911370.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(10040..11635)
                     /locus_tag="ACHK1J_002110"
     CDS             complement(10040..11635)
                     /locus_tag="ACHK1J_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7911371.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(11780..12538)
                     /gene="aroD"
                     /locus_tag="ACHK1J_002111"
     CDS             complement(11780..12538)
                     /gene="aroD"
                     /locus_tag="ACHK1J_002111"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO7911372.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(12569..13435)
                     /gene="ydiB"
                     /locus_tag="ACHK1J_002112"
     CDS             complement(12569..13435)
                     /gene="ydiB"
                     /locus_tag="ACHK1J_002112"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO7911373.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(13447..14712)
                     /locus_tag="ACHK1J_002113"
     CDS             complement(13447..14712)
                     /locus_tag="ACHK1J_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911374.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(14939..16153)
                     /locus_tag="ACHK1J_002114"
     CDS             complement(14939..16153)
                     /locus_tag="ACHK1J_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911375.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(16253..16609)
                     /locus_tag="ACHK1J_002115"
     CDS             complement(16253..16609)
                     /locus_tag="ACHK1J_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO7911376.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(16745..16852)
                     /gene="rprA"
                     /locus_tag="ACHK1J_002116"
     ncRNA           complement(16745..16852)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK1J_002116"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(17038..18150)
                     /gene="ydiK"
                     /locus_tag="ACHK1J_002117"
     CDS             complement(17038..18150)
                     /gene="ydiK"
                     /locus_tag="ACHK1J_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO7911377.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            18539..21595
                     /locus_tag="ACHK1J_002118"
     CDS             18539..21595
                     /locus_tag="ACHK1J_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO7911378.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLMR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            21592..22002
                     /gene="menI"
                     /locus_tag="ACHK1J_002119"
     CDS             21592..22002
                     /gene="menI"
                     /locus_tag="ACHK1J_002119"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO7911379.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGYDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            22102..22290
                     /locus_tag="ACHK1J_002120"
     CDS             22102..22290
                     /locus_tag="ACHK1J_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO7911380.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            22839..23207
                     /gene="sufA"
                     /locus_tag="ACHK1J_002121"
     CDS             22839..23207
                     /gene="sufA"
                     /locus_tag="ACHK1J_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO7911381.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            23216..24703
                     /gene="sufB"
                     /locus_tag="ACHK1J_002122"
     CDS             23216..24703
                     /gene="sufB"
                     /locus_tag="ACHK1J_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO7911382.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            24713..25459
                     /gene="sufC"
                     /locus_tag="ACHK1J_002123"
     CDS             24713..25459
                     /gene="sufC"
                     /locus_tag="ACHK1J_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO7911383.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPGLCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            25434..26705
                     /gene="sufD"
                     /locus_tag="ACHK1J_002124"
     CDS             25434..26705
                     /gene="sufD"
                     /locus_tag="ACHK1J_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO7911384.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGVKRSPQAQQHLEQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            26702..27922
                     /gene="sufS"
                     /locus_tag="ACHK1J_002125"
     CDS             26702..27922
                     /gene="sufS"
                     /locus_tag="ACHK1J_002125"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO7911385.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLEMLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPEDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            27935..28351
                     /gene="sufE"
                     /locus_tag="ACHK1J_002126"
     CDS             27935..28351
                     /gene="sufE"
                     /locus_tag="ACHK1J_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO7911386.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            28500..29504
                     /gene="ldtE"
                     /locus_tag="ACHK1J_002127"
     CDS             28500..29504
                     /gene="ldtE"
                     /locus_tag="ACHK1J_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO7911387.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(29568..29804)
                     /gene="lpp"
                     /locus_tag="ACHK1J_002128"
     CDS             complement(29568..29804)
                     /gene="lpp"
                     /locus_tag="ACHK1J_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO7911388.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         7594 a         7379 c         7626 g         7295 t
ORIGIN      
        1 tatatttgct tgaacgattc accgtttttt tcatccggtt aaatatgcaa agataaatgc
       61 gcagaaatgt gtttctcaaa ccgttcattt atcacaaaag gattgttcga tgtccaacaa
      121 tggctcgtca ccgctggtgc tttggtataa ccaactcggc atgaatgatg tagacagggt
      181 tgggggcaaa aatgcctccc tgggtgaaat gattactaac ctttccggaa tgggtgtttc
      241 cgttccgaat ggtttcgcca caaccgccga cgcgtttaac cagtttctgg accaaagcgg
      301 cgtaaaccag cgcatttatg aactgctgga taaaacggat attgacgatg ttactcagct
      361 tgcgaaagcg ggcgcgcaaa tccgccagtg gattatcgac actcccttcc agcctgagct
      421 ggaaaacgcc atccgcgaag cctatgcaca gctttccgcc gatgacgaaa acgcctcttt
      481 tgcggtgcgc tcctccgcca ccgcagaaga tatgccggac gcttcttttg ccggtcagca
      541 ggaaaccttc ctcaacgttc agggttttga cgccgttctc gtggcagtga aacatgtatt
      601 tgcttctctg tttaacgatc gcgccatctc ttatcgtgtg caccagggtt acgatcaccg
      661 tggtgtggcg ctctccgccg gtgttcaacg gatggtgcgc tctgacctcg catcatctgg
      721 cgtgatgttc tccattgata ccgaatccgg ctttgaccag gtggtgttta tcacttccgc
      781 atggggcctt ggtgagatgg tcgtgcaggg tgcggttaac ccggatgagt tttacgtgca
      841 taaaccgaca ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa
      901 aatccgcatg gtttacgcgc cgacccagga gcacggcaag caggttaaaa tcgaagacgt
      961 accgcaggaa cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa
     1021 acaggccgta caaattgaga aacactacgg tcgcccgatg gatattgagt gggcgaaaga
     1081 tggccacacc ggtaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg
     1141 tcaggtcatg gagcgttata cgctgcattc acagggtaag attattgccg aaggccgtgc
     1201 tatcggtcat cgcatcggtg cgggtccggt gaaagtcatc catgatatca gcgaaatgaa
     1261 ccgcatcgaa cctggtgacg tgctggtcac tgacatgacc gacccggact gggaaccgat
     1321 catgaagaaa gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat
     1381 catcgctcgt gaactgggca ttccggcggt agtgggctgt ggtgatgcaa cagaacggat
     1441 gaaagacggt gagaacgtca ctgtttcttg tgccgaaggt gataccggtt acgtctatgc
     1501 ggagttgctg gaatttagcg tgaaaagctc cagcgtagaa acgatgccgg atctgccgtt
     1561 gaaagtgatg atgaacgtcg gtaacccgga ccgagctttc gacttcgcct gtctgccgaa
     1621 cgaaggcgtg ggacttgcgc gtctggaatt tatcatcaac cgtatgattg gcgtccaccc
     1681 acgcgcactg cttgagtttg acgatcagga accgcagttg caaaacgaaa tccgcgagat
     1741 gatgaaaggt tttgattctc cgcgtgaatt ttacgttggt cgtctgactg aagggatcgc
     1801 gacgctgggt gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg attttaaatc
     1861 gaacgaatat gccaacctgg tcggtggtga gcgttatgag ccagatgaag agaacccgat
     1921 gctcggcttc cgtggcgcgg gacgctatgt ttccgacagc ttccgcgact gtttcgcgct
     1981 ggagtgtgaa gcagtgaaac gtgtgcgcaa cgacatgggg ctgaccaacg ttgagatcat
     2041 gatcccgttc gtgcgtaccg tagagcaggc gaaagcggtg gttgaggaac tggcgcgtca
     2101 ggggctgaaa cgtggtgaga acgggctgaa aatcatcatg atgtgtgaaa tcccgtccaa
     2161 cgccttgctg gcagagcagt tcctcgaata tttcgacggc ttctcaattg gctcaaacga
     2221 catgacgcag ctggcgctcg gtctggaccg tgactccggc gtggtgtctg aactgttcga
     2281 tgagcgcaac gatgcggtga aagcactgct gtcgatggcg attcgtgccg cgaagaaaca
     2341 gggcaaatat gtcgggattt gcggtcaggg tccgtccgac cacgaagatt ttgccgcatg
     2401 gttgatggaa gaggggatcg atagcctgtc tctgaatccg gacaccgtgg tgcaaacctg
     2461 gttaagcctg gctgaactga agaaataaaa taaatacccg gcggcgtttt gtcgccgggt
     2521 ttatgtgatc cccgaagatg aaacttattc aatctcttca cagacatcct gagttaatcg
     2581 ccgcataata tctttcctta acaaaaactt ttgtatttta cctgaggcag tgcgcggtag
     2641 tttttcgatt accacgatat gttcaggata tttatatttt gcgacccgtt tacggctaaa
     2701 aaaagccacc acctcttcca gcgataatga atgatgcggc gctttcagca cgacataagc
     2761 gcatgaacgt tcacctaagc gttcatcggg cattgcaacc acacaagcat cgtgaatttt
     2821 aggatgctgt aataaaatat cttccacttc acggctgcta atattttcgc cgccgcggac
     2881 aataatatct ttcttgcgcc cggttatttt tatatagcca gcctcatcca tgcggcagag
     2941 atcgccgctg taataccagc cttcttcatc cagggcacgg gcggttaatt caggttcatc
     3001 aaaatacccc ataaacacat tggggccacg cgaggcttct tcaccttcgc aacctggcgg
     3061 taaggtcttg cgtgcgtcat cgaccacttt aatctctaca cctgcggcag cgtaaccatc
     3121 ggtgtgcata aagcgcgaca aaggatcatc gagattcacc accgcatgcg gcgaactttc
     3181 tgtggaacca taaacactta ataatttaat gccgcgctgc tggcattcac gcgccacttt
     3241 tttggggatt gtggtaccgc cgcaaagaaa gaagcgcagc gctgaaaggt ctgcgggttg
     3301 tttctctagt aaattcaaaa gatcatagac aaacggcgtt gcgccgagca tacaggtgca
     3361 acgctgctgc tcaagcagcg cgagacacgc atcaggagtg aaaatatcta acaacacgct
     3421 gcgagcgcca attaagaatg gtgccgttac gccatgcaga aagcccgttg cgtggccgag
     3481 tggcgcaggc atcataaaga catcctgcca ggtcagattc agccgcgcgc aataagcccg
     3541 ctcactggcg agaatattgt tatgcgttag catcacgccc tttggcagac cctcggttcc
     3601 ggaggtaaac agcaccgcgg ctaattcatc gccgtggacc gttatcgccg tggtcagtgg
     3661 ggtattgtcg gcgataatct gacttaatga gagggaagag gtggcgggag ccagtttgtc
     3721 cacgccgaca atttgttgta gttgtggaag ctgattttgc agcggcagga ttaaatctac
     3781 cggacgcgtt tgtttaaaca acgtcggtgc aaagaacatt tttgcctgac acttattgag
     3841 cacccacacc agttctgctt cctgccagga aggcaacagc ggcacggaaa ccgcaccgat
     3901 tttcaggcag gcaagataga taacggtaaa ttcacaccag cctggcagtt gaaatgcgat
     3961 gcgatcgcct gattcaatac ccttcgctaa catccagttt gccagacagc tcgcggcgtg
     4021 atcgagcgcg ctataggtgt acgatgctcc atgattatcg accacggcaa ttttgtctgg
     4081 catcgcacga gcggtctgct gccagtaatc ggccagcgaa gcatcgcccc atagcccttg
     4141 ctgacgatac gccgcacgac gttgttcgtt aaacgttaat gtcactttca ttttggactc
     4201 tcgaaacaga acatcaggcc cgagatgcgg gcctgtggga tgcatgatta gccgtagcga
     4261 aaatcaatac cgaaggtgcc tgcgggatat tgccactgtt cgagaatagt gttaccgcac
     4321 aacacccgac aggtgccgca ctccagacaa ccggcagaat caaaatgaat gttgccggcg
     4381 tcatcctgct tataaagtcc ggcagggcag gctttcatta atttttgaaa ttctttgatg
     4441 tcgggatttg ccgccagaat aatatgtgga tggccctcat caacatggaa tttattgacg
     4501 cctaatttga catcaacgtt aaccgtagcg ttctggctca tagcgcggtt gctcccttaa
     4561 tgccatcttt cagcagattg atcagcccga ttttcttcgc gtgtcccatg atcattttgc
     4621 gtaccggctg gtttggtttg ccgtcaatgg tgaacatatc gttcatgatg tcggcgatca
     4681 tgcgcggata ctgactaaac aggcgcggat tttccatcag cgccgggatc ttgcgaaaat
     4741 ggtgcatatc gcgcataaca caactttgtt ccagctcacg tttgtattgc gcaagactgc
     4801 tggcggagaa atccgcgcgt tctttggcgg cgatcaccgt tgtggcggca gcctgagccg
     4861 atgcaatggc taaatccatg ccgcggaccg taaaacccaa attcaggcag aagcctgcgg
     4921 cgtcaccaac gatcatcacg ccctcgttaa ccagttgcgg caccattgcc agaccgcctt
     4981 ccggcaccat atgcgcggaa tattcaagca gtttgccgcc gctaatcagc gggtgaatgg
     5041 cggggtgttg tttaaaatct tccagcattt gcggcacgct tttttgcgca tgggcgatat
     5101 cacccaatcc acaaaccagc cccaacgata tggaatcctt attggtatag aggaatccgc
     5161 cacccatcag gccgtcagaa ggggaaccgg caaacagcca ggcggcacct tcctcgcccg
     5221 taatattaaa gcgatcgttg atctgttctg gtgtgaggcc aataacctct ttaacaccaa
     5281 cagcgtaatg atgcggatcg gaagcgggaa ccattcccag cgaacggcca agcatcgagt
     5341 taacgccatc agccagaatc accacattcg cttcgagaat atcatcccca gcctggacgc
     5401 cagtgacctt gtttccttca cgaaccagcg catcgacgcg aactcccggg ataaactgtg
     5461 cgcccgcctg ctcggcttgt tccatcaacc acgggtccag acgattacgc aatacggtat
     5521 aagatgcgtg ttgcggaaca tctggttgct cgcggtgaaa atcgagggta acggcgctct
     5581 cttcggttaa gaaggagatt ttctcgcgtg tgaccttgcg ttctaccggc gctgaggctg
     5641 caaagcctgg aatgattgct tcaagtgtgt gggcataaag acgcccgccg gtcatgtttt
     5701 tacatccggc actgtcgccg cgttctatca ccaggacatc cagcccggct cgtgccatga
     5761 ccagtgcggc aacgctacca gcaacgcccg caccgaccac aatggcatca aatttgtcat
     5821 ccgacatgcg ttatgctccc ttcccgctgc gtttacagcg ggagatgtgg ttgaaagggg
     5881 aattagcggc tcaactggct aatcagggca gggacgactt tgtagatatc gcccaccaga
     5941 ccgtagtcgg catagttgaa gattggcgca tttttatctt tattgatggc gacaatcact
     6001 tttgcgccgt tgccgccaac catatgttgg atctgcccgg agatccccag cgtcaggtag
     6061 agatcggatt tcagcaacac gccggagaca ccgatataac gttcacgctc catccagttc
     6121 tcgccttcgg caattggacg tgaacagccg acttcagcat tcagcaccgc cgccagttcg
     6181 tggaccattt tcaggtcatc ctgcgctgcc agaccacgac cgacgccaac cacacgtttt
     6241 gctttgctca ggtccacgct gcttgccgct ttcgcacggc gctcgcgaca gagaatttca
     6301 tgacgcggag caacataagg taccgtttct gtcgggcact gatgagaggt atcactggtg
     6361 cacggttcct gaacaccggg tgcaagggta atgatcgcta gcgagctgtt gatcttttcc
     6421 tgagcgaacg ctaacccgcc atacatccgg tgttcggcgc aaatgtgacc atcgacaata
     6481 tccaccgccg tggcatcgtt caccagcgcc gcattcagtt gcacacttaa ccgtgctgcc
     6541 agagctttac cacgtttcgt cgcggccagt agcaacatag cggggtgttt atctttcagc
     6601 agagcagcaa tgctttcggc gtaattttca gtgcgttgca gcgcgtcgtt ttgctcaaga
     6661 acataaatac attttggacc ataaggcata actgcctgca cctggtcggt attttgtaca
     6721 atggcataca cctgttggcc ccattgctga gcgccgccaa acagttcagc ataacgttca
     6781 ggattatcgc taaagaccca gacgctgttt aattgactca tcgtttcttc tccgtaggct
     6841 tcagttcagg gctttcttca gatgttccgc cagctcggca atggcctccg gcgaatcgtt
     6901 atcgagaatg atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc
     6961 aagtggtgcg ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt
     7021 acccgcgccg agaatggctt tcatcgaagg aatgcgtggc acgttgatat cggaggtgac
     7081 gcagagcacg gctggaacag agagttcaat aacttcaacg ttatcttcaa gcgtgcgttc
     7141 aatcaccagt gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg
     7201 cagaatttct ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcacc
     7261 aaagatcagt aaatcgaagc cgatcttttc aactgctgcc gccagcgctt ttgcggtatc
     7321 gagaggcagt gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg
     7381 ggataacacg tctttgcgca ctttcgagtt ctgcaacaat gagccgccaa cggtcagcgc
     7441 ggctatctca tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa
     7501 ctggctgatt ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat
     7561 gtcctgttct tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag
     7621 tcgcatacgg aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc
     7681 ggtgcggggt tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
     7741 cgatcgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
     7801 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
     7861 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
     7921 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
     7981 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
     8041 gacaatgaaa ccgagctgat ttacgtcaag aaaggggtgg caaggtttac catcgattct
     8101 tcgttatatg tcgcgcatgc agatgatata gtggtgatag aacgcggcag gctgcatgcg
     8161 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
     8221 tttcaggggg ctgaggaaaa tcatctactg caaccgcatt cttgtccggt aattgccgcg
     8281 gggcagggaa aagaagtcat taaaacctta tttaatgagc taagtgtgat tttgccgcaa
     8341 agtaaaaata gccaaacatc ttcgttatgg gatgcatttg cctatacatt agcaattctt
     8401 tactacgaaa actttaaaaa tgcttatcgt tcggagcagg ggtatattaa aaaagatgtt
     8461 ctgataaaag atattctttt ctatctgaat aataattatc gcgaaaaaat cactttagaa
     8521 cagttatcga aaaaatttcg tgccagcgtc agttatattt gccatgagtt taccaaagag
     8581 tatcgtattt cccctattaa ctatgttatt caacggcgta tgacggaagc gaaatggtta
     8641 ctcactaata ctgaattatc acaggcagag atctcctggc gtgtgggtta tgaaaatgtc
     8701 gatcactttg gcaaactgtt tttgcgccat gtcggctgtt cgcccagcga ttaccgcagg
     8761 caatttaaaa actgttttgc ggaacaagaa atcctatctg aatttcctca accggtaagt
     8821 cttgccggat aaatctggcg gcgcagtttg ttaagaagct gcgccgcctg cagattattt
     8881 gttctgatag tctttcagga tctgccgacc tgctacgtaa atcataattt cgtctgtacc
     8941 gccgccgata cgttcacaac ggacatcacg ccagaagcgg gagacgcgcg cttcatcggt
     9001 atagcccaga ccgcccatga tttgaatcgc atcatcaatg acttccattg cggtacgtgc
     9061 gcaatacagt tttgcaagcg ccgcgctggt gcgcagtgac tgatgctgat cggcttgcca
     9121 tgccactttc agcaccatgt tgcgcatgtt gtcaatctta atcgccatca gcgccagttt
     9181 ttcctggatc atctggttat gaccaatggg cttaccaaaa gcgatacgtt ggttggcata
     9241 gcgggcggca tcttcaaagg cgcattcggc aaagccggtg ctgcgcgcgg cgttgatcag
     9301 gcgctccatc tcaaagttgt acatcacatt gaggaaaccc attccttctt cgcccaccat
     9361 gtcgctctct tcaacttcca cgttgtcgag atagacttcg caggtgctga gcatatgcca
     9421 gccgatttta tgcagcgggt taatcttaat gccgggctta ctggagtcga cccaccacag
     9481 ggtgaaggct tttttgggat ctttcggttc aggatcgcgc gccagcacca gcatatacgg
     9541 gtactctttc gcaccggtaa taaaggtttt ctgtccgttg atgtaaacct tgccgttttt
     9601 acgcgtgtaa gtggtagtgg cactgttgtt gtctgagcct gcgcctggct ccgtcaacgc
     9661 cagggcatag gcgggatcac ccgtttccag tgtgctttct gccgttttac gtagctgctc
     9721 tgcagaaccg aaacggcgca tactgtgaat acattgaccg ttggtaatca aaaatgccgg
     9781 agcaccgcat tttgacactt ccatcagtgc cagcatttgg gtgacgtaat ccgcaggtat
     9841 accaccaaat tcttccggca cgccaagcat ggaaataccg ttatccgcca gtgcccgcat
     9901 aaattcacgc gggtatgtcc cgttttgatc gcaggtgcgg aaatactctt ccggaaagtt
     9961 agtcgtaatc agttcgcgaa tactggccag cagcagttct tgttcttcag ttaaagaaaa
    10021 atccatcgtg atactcctgt taatgagccg cttcaggcag gacaaaaccc atcgccgcat
    10081 cgataaataa tctttcgtcc atcagtttga gttctggcga aatcactggg gtgaaatcca
    10141 ttttgtcgag aatatctttt tgtaaatcga cgccaggggc gatttcgatt aaatgcaggc
    10201 cgtcttcttt cagcgtgaat actgcgcgct cagtgatata acgaacatcc agccctcgct
    10261 cgagagcgat ttttccgctg aaagtaattt ccggtagttc ccgaataaat ttcttcaccc
    10321 gtccttcctg gacgatattt aatttgccgt cggtaatttc tgtttttaaa ctgcccgcag
    10381 ttaatgtgcc gcagaaaatg attttcttcg aagtggcact gatatcaata aatccaccgg
    10441 tacccatgat tttgccattg aatttatgca cgccgacgtt accgtgctgg tcgacttcag
    10501 caaaactcaa ataacaaaca tccagaccac caccgtgata aaaatcaaac tgggacgtca
    10561 tatccagaat ggcgcgggta ttcacgttcg cgccaaaggc gataccctgt gaagtaattc
    10621 cgccaatcgg acctgtttct accgtcagaa taaagtcatc agcacaacct tcttcccggg
    10681 cgaccaggcc aatgccgtca gcaataccga cgccgacatt ccccaccgcg cctttacgca
    10741 tttcgaataa tgcgcgccgc gcaactaatt tacgttggtt taggggcagg ctaagtttgg
    10801 tactgtcatc aagtgtgaag tcaccagaaa taaagcggtt aaccggtgca ccgccataca
    10861 gttgggtttg atccggatcg accaccacaa tatccaccag ataacccgga atacggacag
    10921 atttaggatg cagcgtggct ttcttaacca ttttctgcac ctgcatcatc acaataccgc
    10981 cgttattgtg caccgcttgg gcaataacca atgcgtcgag atacatcacc tcatcttcaa
    11041 aagtggcgta gccttcactg tcgcaggtgg tagcgcgaat gaaagcaata tctggcgcaa
    11101 tcgctttgta atagagatat tctttgttat caaactcgac cagtttaatc aggtcttctt
    11161 tagtgacttc attcagtttg ccgccttgct ggcgtggatc gacaaatgtc ccgatgccaa
    11221 tatcactaat aataccaggc tggtgggctg cggcggcgcg taaggtttgt gtaagtacac
    11281 cttgtgggta gttataagca ataattttat tttgttctgc gagttcagaa atacgcggcg
    11341 attgtcccca gtgaccacat aatgcccatt tcaccagacc ttcttgcgcc agaggactaa
    11401 taccacggtc ggcgcgatcg ccaagccctg ttggactaat aatcgataaa ttacgtggcg
    11461 tttgagtctg tttatattta tcagcaagag cagtaattaa cgtggtggct tccagaatac
    11521 cgccgccagc gcctaacaca caaagtgttg cttcgtcggg aatataattc accgcttcct
    11581 gtgccgacag gaccggcact cgtccattaa tacgaggtgg ttttacaggt ttcattgtta
    11641 attcctgatt caggtgtgat tttccctacg ccagatggat aattagcgca cagagactca
    11701 cgaaatattt ttattaaata cggttgaata tattttttta gttcggcggg ggaggatatt
    11761 cccgccgaaa tattattgct tatgcctgat gtaaaatagt taatactgtg cgcaaatcat
    11821 ttaccgagat ttgccctggc gcagaggctt tttttaccgc accaaaagtt gccgccgacc
    11881 caaatacttc accagccaga cgagaaatta cgccagtttt tgccattgac atcgtgatga
    11941 ttggacgatc ggcatactgc tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat
    12001 cgctggtact ttgcggcatc agcgcaatct taggaatatc ggcgtcgaag gactgcattt
    12061 tgcgcagacg ggcaatgatt tcttcggctt ccggcgtttt atggaagtca tggttggaca
    12121 tgacaacttt cacatcatgc gcgtgggcgt aggcgacggt ttctttaacc tgatcatcgc
    12181 cggtaaataa ctccagatcg atcatatcaa ccaggccgct gtcgatggct gcacgattga
    12241 gagcaatata agcctcggtg gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg
    12301 taaacagcag cggtttttct ggcatggttt cacggagaat ttttgccgcc gccatgacag
    12361 actccacatt ggagaggtcg gcaaagtggt ccacacgcca ttccagaata tcaaagtccg
    12421 cttcacgata ggcgagagct tcggatttca cgcgggcgat atctttcgcc atcagcgaga
    12481 cgatgatttt aggtgcgccc gtaccaatga cgagatcttt tacagttacg gttttcattt
    12541 tttacccttt ctgcacgcgg tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat
    12601 attccagagg gaaatctttg ccagtccata acgtgaactg ttcagcccct tgccacaaca
    12661 acatgccgta tccatcaatc gttttgcaac cagcttgttg cgcctgctgc aataacttcg
    12721 tcatatgcgg gttatacacg cattcagtga ccagaagtcc cggatgtaac agactgatat
    12781 cattaaccaa tgattcattc tcaaggggtt tcatacccac ttttgtgcca ttggttaaaa
    12841 tgtcggcgga agccagggct tcagcaaagg cttgctgatc ggcgagatcg gtaaccgtga
    12901 cgacacaatc ggtgttttcg ttaacccgct gcgcgaaggc gagggcttta tcgaagaact
    12961 catcccgacg gttaaagagt ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa
    13021 tcgccgttga ggcacctccg gcccctaaca gcaccattgt tttgcctttg atatcaaaac
    13081 cgctctcttt aatggcgcga atatggcccg tgccgtcggt gttatagccg cgcaggtagc
    13141 catcatcatt aacgatggtg ttgatggcac ccaccagttt ggctgccggc gttaattcat
    13201 caacatattc acacgccagt tgtttgttcg gcattgatac accagttccg cgcattttta
    13261 gagcttttaa tccttcaatt gctccgggaa agctatcgtt atccacttcg aaggccatat
    13321 aggtaaatgg caatcccgct ttttctaagg ctttattctg catttcaggc gataaactgt
    13381 ggcggatagg ataagccatc aacccaatca attcgtattt tgcggtaaca tccataatta
    13441 actcctttaa cctctatgct taattgtgtt taaccgggtg ctaaatttgc gctcgccaaa
    13501 ccgcacatca ttctcaggaa taataaaaac gcggtaatag cggataaaaa caataattgc
    13561 ggtaatcagg gccagcagcg cgaaagtaaa atcgagaaca atgatatatt gcaggccgat
    13621 gttcgacaga taaccggtaa tcagcggaat aacaaagtta gccagtccac ccatcatcat
    13681 ataaatactg gtgactttgg ctttgctttt tgggaaaaac tctgacatga ccgaaacacc
    13741 gagctgtaaa atgccgccag ctgctgagaa accgataaca aaggcgccgg cattacacac
    13801 cagtggagaa gggtacaggt agataatggc tgctgttatt gttgccagtg cagagttaaa
    13861 tacattagcc cagatgggcc ggaccatttt tttcagtagt gcggcaaaaa taaagacaca
    13921 gaccaacgag cccatactgt aataagagat ggtttttaat gcctcagctt ctgacatacc
    13981 agcaaaagcc atcgcatatt tgggcatcca caccacaatc acataaaagg tcgagaatgc
    14041 ggctacaccg aacagtaccg atgaaacacc ttccagccag actaacggtt tgctgttcat
    14101 ttgcggtaat tcattagcta cgctggcgtc caccaactgg ctggggaatt tgcttttcaa
    14161 cagcatcagc gtgatcagta caaacagaat acccggaata atcagcccat agccgtacca
    14221 gatattattg agcaacatat agctcaccag cattgggtag aacatttgcc caaatgacac
    14281 catcgcttta accagtatga ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc
    14341 ggggtagcca cccgtatcca gcgctgagtt agcgatacct acacacactg ccagaccgta
    14401 ggcgagagtt aaattcgggc aagcgggaat accaaagaag aatagcagat acattattac
    14461 tgccattaat atcaccgccc gacgaccaaa cttatcggag atcacaccga agaataaaat
    14521 actgatcaat cgccccaaac cgataccgga aattaagtag gcaatgcccg cgttgtcagt
    14581 ggaaaacttt tccgccagag atgacatatt ttgggcaagc gtaataacac taatgccgtg
    14641 caggaagtag ctgaagtaaa tacaaagaac agccaggata aatggcgtgc tgaaagcctt
    14701 attttgagac attttcacag ctccttgctg aaagataatt tcgttaccgt acgtattgtt
    14761 ctccgacggt ttacataatt actatactga agatgatttt ccctgggtac atggcataaa
    14821 gtttatgttt ccaggaatat tgcatgtcat aatttgtttt tgatcgcgat tttccttagt
    14881 tagcgcagaa agagttattt atccgtaaga tcttgcatga cctgaaaaga atattgcatt
    14941 acccacccgg agcgacattt tcctttagcg agtgatgccg cgtttttttg cggctgcgta
    15001 agccgataaa cagtgccagt aaaaagccga tggcagcgat ggcggtatcg aaccacataa
    15061 tatcggcaat acttctttgg gacagatgag ccgtaatcaa cggaatagta aaggtcgcaa
    15121 tactgcccgc actgtaatag atccctgtag ctttaccttt agcgtagggg aaacgttcag
    15181 ccattaacgt caggccaatt tgcacaacac ctccagcaga ggtaaaacca atgacaaaag
    15241 caaatattat caccacataa aatgtgggat gcaggcagac ggtaaacaga gcaataaatg
    15301 agataaaggt gtacagcatc agtaatgttg ttgggcgaac ggtattacga atgagtggag
    15361 cggtaataaa tacacaaagc agcgaaccca cggtgtagat actgagtagt ttgatcgaca
    15421 tagtgtatga catgcctgca acaaattgtc cgtactgtgc cagccactgg ctaaccagat
    15481 aaaacgttgc cattgagata tagccatata aggtataact ggctaaatcg ataattgaac
    15541 agcgatgttc cgtagagctg gtggttttct ttatgacagg taagcgacga cccggatggg
    15601 gggggaacgt acaacggtat aaaaacagag cgttaataaa cataatgcct gcagcaatca
    15661 taaaggacca accgaaccac agttcagccc acactaacag gctaatgatt agcggtaata
    15721 aaaattgtcc gctggaaaca aatgctttaa ttaaaatatt ggctgtccca ggtgagcgtg
    15781 gaaaagcttc catcaaactg ggataagtgc ctgcatcgag aaaactgttt gccattcccg
    15841 ccagaaagcc aaaaacataa gcgataatga tgttattggt ctgcaggatg ccaaaaaaga
    15901 aggccatata gcagcacatc ccgagcatga taaaagggcg gcgaccaaag cgatcggata
    15961 ataatcctgc aaaaagcaag acacttaatc gaccaatgcc cagcgatgag ataactatcg
    16021 agacacccgc ggcattagtc tgccaaagtg tctccagcga ggccatattc aggctcatca
    16081 aaatgacgcc cataccatgc accaggtaat taaaatacaa cccaagtgcg gtagggtaat
    16141 agggattttt cataggttct acttagcaaa ggtgctttaa aaagtgtagc gattttgtgt
    16201 cagtatccgt tcattattgt tgtaacaacg acaattcctt tttgtggaga aattaacaaa
    16261 gccgctccag atcgtgtgca tataattcag cggctaccga ttgatagatc ttccagtcaa
    16321 taaaattgcc atccgggtag acacgttgaa acgcggttaa gtcggggaaa aaacgcatgg
    16381 tgttgttgcc gatgcggtta tttattttct ctataatggc atgaagatgt ttttttcgtt
    16441 gctgacgcat agccaacagt tgctcgacta cacgatcagg aatggcgcat ttgccatttt
    16501 cccactgacg ccagctttca ctgtcaccgg tctgggcaat ccaggtcgcg cattcatcaa
    16561 tagtcatggc aaaaatatgg cggagtgcct ggagttcata agcgttcatc gtgtaatggc
    16621 tgcatgaaat aaacaataaa atataaataa cattatcagc ctgctgacgg cttgaagaga
    16681 gtcacagtat cttgtgcaac attattggaa agagtgaggg gcgaggtagc gaagcggaaa
    16741 aatgttaaaa aaaagcccat cgtgggagat gggcaaagac tacacacagc aattcgttgt
    16801 ttcactcagg ggatttccat gcttataaat caatatgttg atttataacc gtgagctaat
    16861 agtaggcata cggacttttt gcgtcgatca gattcgtctc attagtttaa aaactgtaaa
    16921 gaatttgcga caggtactac cagctgaatg gcggtaagca ggaaaatatg gggaagagca
    16981 agcaaatatc aattagcggt ggttaacaat caggcggcag agccgcctga tgagaaatta
    17041 cttattcggt ttctcgattt cgccaagttc ttcgagaatt tcttccggtt ggtccgtcgg
    17101 cggcgggact tcttccaccc acgcggcaaa cagacgccag gaaacggcta acagaaccgg
    17161 accaataaac agaccgatca tcccgaaagc aatcaaacca ccaataacgc cagagaggat
    17221 caggatcagc ggtaaatcgg cacccatgcg aattaacatt gggcggatga cgttatccag
    17281 cgtgccaacc acaccgctcc acactaacaa taccgttccc caggtggtat cgccggtcca
    17341 gtagagccag ataatcgccg gaatcagtac cggcaacggg ccaagctgga caaggcagga
    17401 gaggatcatt aacaccgtta gcaacgttgc ataaggtacg ccggatacgg cgaggccgat
    17461 accgccaagc actgcctgta ctaacgccgt caccaccaca cccagcgcca ccgcgcggat
    17521 agcctgtgcc gccagcagca cggcggcatc tccgcgaacg cctgccagac gggtcgcaaa
    17581 atggcgaatg ccttgtgcca cctgttcacc gcgccagtac agcagggcac tgaagagaag
    17641 catcagcgca caatgcacca taaagcgccc gatatgcgcc gcttgcccaa cgaaccaggt
    17701 ggtggtggtg ccaatataag ggcggacttt cgccatgatc gccgtccccc ccatatccag
    17761 caagttgtgc cagcctgcat acagcttcgc gccaatcacc ggaatggtat taagccacgc
    17821 cagatcgggt aacgtcatgt caccgctgga aatggcttta attagcgggc cgctgccgtc
    17881 gacgatactg ttaaccagca aagcaatagg gatgataaac accatcacta ataacagcgt
    17941 catcaccaga acggcgagag agcggcggcc aaacatgatc ttttgcaaac gtaacaatac
    18001 cggccaggtg gcgataacca ccgtaccggc ccatgcaaag ccgagaataa agggttgaac
    18061 aatccacaga catgccacaa tcatgatggc taaaaacagc accgaaagca gaatttgtgc
    18121 gacatccctg ggctgacgaa catttaccat aactactttt cacctttttt gtgcgtcaaa
    18181 tcgtcgacgc gacgtgaaca cgcgaaactc acctgcataa tgatgagcaa tttcagcggt
    18241 ttttaacagg ccgattctgc ccttaattct gatgggcgtg ttagaaaaaa atgtggtaca
    18301 acaactcagg caacacgcaa acgatttact cgtcgtattt caagttgcat atgctgcggc
    18361 tacgttcgtt caccccagtt acttactgtt gtaagctcct ggagttcact cgcttgccgc
    18421 cttcctgcaa ctcgaaatcc atagagtaaa taaacatcac ctttctgtgc gctattgcag
    18481 ccgcctgaaa gaacagtgta tcgacctaca tcacagaccg caggaaaggg tcaatataat
    18541 gattccacag atttcccagg cacctggcgt cgttcaactg gtgcttaatt ttttgcaaga
    18601 gctggagcaa caaggtttta ccggcgatac ggcgacaagt tatgccgatc gtctgacaat
    18661 gtcgaccgac aacagtattt accaacttct ccccgatgcg gtggtatttc cgcgttcaac
    18721 cgcagatgtg gcgctgatcg cccgtcttgc cgcgcaggaa cgctattcat cgctgatctt
    18781 taccccccgc ggcggcggca ccggcactaa cggtcaggcg ctcaaccagg gcattattgt
    18841 tgatatgtcc cgccatatga accgcatcat cgaaattaac cctgaagagg gctgggtgcg
    18901 cgttgaggcc ggggtgataa aagaccaact caatcagtac ctgaaaccgt tcggctactt
    18961 ttttgcgccg gaactttcga ccagcaaccg ggcaacgctc ggtgggatga tcaataccga
    19021 tgcatccggt cagggatcgc tggtctatgg caaaacgtca gatcacgtac ttggcgtacg
    19081 cgcggtgttg ttggggggcg atattctcga tacgcaacct ttacccgtcg agctggcgga
    19141 aacgctgggt aaatccaata ccacaatcgg gcgaatttat aacacggttt atcaacgttg
    19201 ccgtcagcaa cgccagttaa ttatcgacaa cttccccaaa cttaaccgct ttcttaccgg
    19261 ttacgatctg cgtcatgtct ttaacgatga gatgaccgag ttcgacctga cgcgcattct
    19321 gacgggttca gaagggacgc tggcctttat taccgaagcg cggctggata ttacgcgctt
    19381 gcctaaagtg cggcgtctgg tgaatgtcaa atatgactct tttgactctg cgctgcgtaa
    19441 cgcgccgttt atggttgagg cgcgggcgct ttcggtagag acggtggact caaaagtgct
    19501 gaatctggcg cgggaagata ttgtctggca ttccgtcagc gagttgatta ccgatgtgcc
    19561 tgacaaagag atgctcgggc tgaacattgt ggaatttgct ggtgatgatg aggcgctgat
    19621 tgatgagcgg gtaaatgcac tctgtgcgcg gttggatgag ctgatcgcca gtcatcaagc
    19681 aggtgtcatc ggctggcagg tgtgccgtga gctggcgggg gttgaacgta tctatgcaat
    19741 gcgcaaaaaa gccgttggtc tgcttggcaa tgccaaaggt gccgctaagc caattccgtt
    19801 tgctgaggat acctgcgtac cgccggaaca tctggccgat tatattgctg aatttcgcgc
    19861 gctgctcgac agccacggct taagttatgg catgtttggt catgtcgatg ctggggtgtt
    19921 gcacgtccgt ccggcactgg atatgtgcga tccgcaacaa gagattttga tgaagcaaat
    19981 ttctgatgac gtggtggcgc tgactgcgaa atacggtggt ttgttgtggg gcgagcacgg
    20041 caaaggtttt cgcgctgaat acagcccggc gtttttcggt gaggaacttt ttgcagaact
    20101 gcgcaaagtg aaagcggcat ttgacccgca taaccgactc aacccaggga agatttgccc
    20161 gccagaaggt ctcgatgcgc cgatgatgaa agtggacgcg gtgaagcgcg gtacattcga
    20221 tcggcagatc cccattgcgg tacgccagca gtggcgcggt gcgatggagt gtaacggcaa
    20281 cggtttatgc ttcaactttg atgcccgtag tccgatgtgt ccgtcgatga agatcaccca
    20341 gaaccggatt cattcaccga aagggcgcgc aacgctggtg cgtgaatggc tgcgtttgtt
    20401 ggcggatcgc ggcgttgatc cactcaaact ggaacaagaa ctgcctgaat ccggcgtcag
    20461 tttgcggacg ttaattgccc gcacgcgcaa tagctggcat gcgaataaag gcgaatatga
    20521 cttctcacac gaagtcaaag aggcgatgtc gggctgtctg gcctgtaaag cgtgttcgac
    20581 ccagtgcccc atcaaaattg atgtgccgga gtttcgctct cgtttcctgc agctctatca
    20641 cacccgttat ttacgtccgc tgcgcgacca cctcgtcgct acggtcgaga gctacgcacc
    20701 gctgatggca cgcgcgccga aaacctttaa cttcttcatt aaccagccgc tgatgcgcaa
    20761 actctcggaa aaacatatcg gcatggttga tttgccgctg ctgtcggtcc cctcgctaca
    20821 acaacaaatg gtggggcatc gctcggcaaa catgacgctg gaacagcttg aagctctcaa
    20881 tgcagagcag aaagcgcgca cggtgttggt ggtgcaggac ccctttacca gctattacga
    20941 tgcgcaagtg gtggcggatt ttgtccgtct ggtcgaaaaa ttaggtttcc agcctgtgtt
    21001 actgccattt tcgccaaatg gcaaagccca gcatattaaa ggttttctta atcgttttgc
    21061 gaagacggcg aaaaagacgg cggatttcct caaccgtatg gcgaagctgg gtatgccaat
    21121 ggtaggcgtc gatccggcgc tggtactttg ttatcgcgat gaatataaac tggcactggg
    21181 cgaggaacgt ggcgagttta acgtcttact ggcgaatgaa tggctggcaa gcgcacttga
    21241 gtcacagcca gtggctacag tcagcggtga atcatggtat ttctttggtc actgtaccga
    21301 agttaccgcc ttgccgggtg cgccagcaca atgggccgcg atatttgccc gttttggcgc
    21361 gaaactggaa aatgtcagcg tgggttgctg cggcatggca gggacttacg gacatgaagc
    21421 gaaaaaccat aaaaattcgc tcgggatcta tgagttatcc tggcatcagg ctatgcagcg
    21481 actgccgcgt aaccgctgtc tggcgaccgg atattcctgc cgtagtcagg tcaaacgggt
    21541 tgaaggcacg ggggtacgcc atcctgtgca ggctttactg gagattatta aatgatatgg
    21601 aaacgaaaaa tcaccctgga agcactgaat gctatgggtg aaggaaacat ggtggggtta
    21661 ctggatattc gctttgaaca tattggttat gacacccttg aagcgacaat gccagtagac
    21721 tcgcggacaa agcagccttt cgggttgcta catggaggtg catccgtggt actggccgaa
    21781 agtatcggtt ccgttgccgg ttatttatgt accgaaggtg agcaaaaagt ggttggtctg
    21841 gaaatcaatg ctaaccacgt ccgctcggca cgagaagggc gggtgcgcgg cgtatgcaaa
    21901 ccgttgcatc tcggttcgcg tcaccaggtc tggcagattg aaatcttcga tgagaaaggg
    21961 cgtttgtgct gttcgtcacg attgacgacc gccattttgt gacgttgtca ccagaaaagt
    22021 gtgacgcata ttgcttttgg tcattatctg cacagtaaac ggtggtatac tgtgcaggtc
    22081 ttgtctaaaa gcgaggatgc tatgtctact caacttgatc ccacccagct ggccattgaa
    22141 tttttacgcc gtgaccagag caatctttca cctgcccaat atctcaaacg tctgaaacaa
    22201 ctagaacttg aatttgctga cctgcttact ctctcttctg ctgaattaaa agaagaaatc
    22261 tactttgcct ggcgtctggg cgtgcattga tcctgactta atgccgcaag ttcgccaaat
    22321 cgacctggat taacctccgt ccaggttcga tgaccttctt aatactgctt atctacccgc
    22381 ctgatattgt tcaaaaataa tactaatcgc agtttgtgtt aaaacggcgg gttagcttta
    22441 tgagattatt cttatcgccc cttcaagagc taagccactg agagtgccgg agataagcgc
    22501 cggatggggt agaaaccctt aagcctgtgt cgcacaggct taagggtttt cttatttctg
    22561 aacgctgcac ttttttacct atctcttaaa tagtcatttt caatgcaact ttttcttttt
    22621 catcacagcc ttaatgctcc ttagataata accattatct aacaatgaga tacctaattc
    22681 ttagcgtgcc tgttaaccca cacatcaggg tctatgctta ttaaaagcag gcaaaaagca
    22741 tgttttacgg taaagcccct gcgtttgctg ggttgaactg ataatcatta tcactaacat
    22801 gctgttatac gctgaaagcg atgaagtgag gtaaatcgat ggacatgcat tcaggaacct
    22861 ttaatccaca ggatttcgcc tggcaaggct taacgctgac acccgcagcc gctgtccaca
    22921 tccgcgagct ggtggcaaag cagccgggta tggtcggcgt gcgcttaggt gtgaagcaaa
    22981 cgggctgcgc gggctttggc tatgtgctcg acagtgttag cgagccggac aaagacgatc
    23041 tgctgtttga acacgacggc gcgaagctgt ttgtcccgct gcaagcgatg ccgtttattg
    23101 atggcacgga agtcgatttc gttcgtgaag gacttaatca gatattcaaa tttcacaacc
    23161 ctaaagccca gaatgaatgt ggctgtggcg aaagctttgg ggtataggcg gtactatgtc
    23221 tcgtaatact gaagcaactg acgatgtcaa aacctggacc ggcggcccgc tgaattataa
    23281 agaaggattc ttcacccagt tagccaccga tgagctggca aaggggataa acgaagaggt
    23341 ggtgcgcgca atttcggcga agcgtaatga gccggaatgg atgctggagt ttcgtctaaa
    23401 cgcctatcgc gcatggctgg agatggaaga accgcactgg ttgaaagcgc actacgacaa
    23461 gctgaattat caggattaca gctactactc agcaccatcg tgcggtaatt gtgacgacac
    23521 ttgcgcgtct gaacctggcg cggtgcagca aactggcgcg aacgcctttt taagtaaaga
    23581 ggtggaggcg gcgtttgagc agttgggcgt tcccgtgcgg gaaggcaaag aggtggcggt
    23641 ggatgccatt ttcgactcag tttcggttgc cactacttat cgcgaaaaac tggcggagca
    23701 gggaattatt ttctgttcct ttggtgaggc gatccacgat cacccggaac tggtgcgtaa
    23761 atatctcggc accgtggtgc cggggaatga caacttcttt gccgcgctta atgcggcggt
    23821 agcctctgac ggtacgttta tttatgtgcc taaaggcgtg cgctgcccga tggaactttc
    23881 cacctatttt cgcattaacg cggaaaaaac cgggcagttt gagcgcacca ttctggtggc
    23941 cgacgaagac agctacgtca gctacattga aggctgttcc gctccggtgc gtgacagcta
    24001 tcagttgcac gcggcagtgg tggaagtcat catccataaa aacgccgagg tgaaatattc
    24061 cacggtacaa aactggttcc ctggcgataa caacaccggc ggtattctca acttcgtcac
    24121 caagcgtgct ttgtgcgaag gcgaaaacag caaaatgtca tggacgcaat cagaaaccgg
    24181 gtcagcgatt acgtggaaat atcccagctg cattttgcgc ggcgataact ccattggtga
    24241 gttttactca gtggcgctga ccagcggtca tcagcaagcg gataccggca ccaagatgat
    24301 ccacatcggt aaaaacacca aatcgaccat tatctcgaaa gggatctctg ccggacatag
    24361 tcagaacagt tatcgcggct tagtgaaaat catgccgacg gcaaccaatg cgcgcaattt
    24421 cactcagtgc gactcaatgc tgattggcgc taattgtggg gcgcatacct tcccgtatgt
    24481 cgaatgtcgc aataacagcg cacaactgga gcacgaggca acgacatcac gtattggtga
    24541 agatcaactg ttttactgcc tgcaacgcgg gatcagcgaa gaagacgcca tctcgatgat
    24601 tgttaacggt ttctgcaaag acgtgttctc agaactgccg ctggaatttg ccgttgaagc
    24661 acaaaaactc ctcgccatca gtcttgaaca cagcgtcgga taaggaataa acatgttaag
    24721 tattaaagat ttacacgtca gcgtggaaga taaagctatc ctgcgtggat taagcctcga
    24781 cgttcgtccc ggcgaagttc acgccattat ggggccaaac ggttcgggca aaagtacctt
    24841 atcggcaacg cttgccgggc gagaagatta tgaagtgacg ggcggcacgg ttgagttcaa
    24901 aggcaaagat ttgcttgcac tgtcgccgga agatcgtgca ggcgaaggca tctttatggc
    24961 cttccagtat ccggtggaga ttccaggtgt cagtaaccag tttttcctgc aaacggcact
    25021 taatgcggtg cgcagctatc gcggccagga aacgctcgac cgctttgatt ttcaggattt
    25081 gatggaagag aaaatcgcgc tgttgaagat gccggaagat ttattgaccc gttcggtaaa
    25141 cgttggtttt tccggcggcg agaaaaagcg caacgatatt ttgcaaatgg cggtgctgga
    25201 accggggtta tgcattcttg atgaatcgga ctccgggctg gatattgacg cattaaaagt
    25261 ggttgccgat ggcgtgaact cgctgcgtga cggcaagcgc tcattcatca ttgttacgca
    25321 ctaccaacgc attctcgact acatcaagcc tgattacgtc catgtgctat atcagggacg
    25381 aattgtgaaa tccggcgatt tcacgttggt caaacaactg gaggagcagg gttatggctg
    25441 gcttaccgaa cagcagtaac gcactgcaac agtggcatca tttgtttgaa gctgaagggg
    25501 tgaaacgttc cccgcaagca caacagcatt tagaacaatt gctgcgtacc ggactgccga
    25561 cacgtaaaca tgaaaactgg aaatatacgc ctctggaagg gctgaccaat agccagtttg
    25621 tcagcattgc gggggagata tccccacagc agcgtgatgc cttagcgtta acgttagacg
    25681 ccgtgcggct ggtgtttgtc gatggacgtt acgtgcctgc gctgagcgat gcgactgaag
    25741 gcagcgggta tgaagtgagc attaacgacg accgtcaggg gttacccgat gctattcagg
    25801 cggaagtgtt tctgcatttg acggaaagcc tggcacaaag cgtgacgcat atcgccgtga
    25861 agcgcggtca acggccggca aagccattgc tgttaatgca tatcacccag ggcgtggcag
    25921 gtgaagaggt gaacactgcc cattaccgac atcatctgga actggcggaa ggtgccgaag
    25981 caacggtgat cgaacatttt gtcagcctta atgatgctcg tcattttacc ggggcacggt
    26041 tcactatcaa cgtcgcagcg aatgcccact tgcagcatat caagctggcg tttgaaaacc
    26101 cgctcagtca ccactttgcc cataacgatt tgttgctggc tgacgatgcc accgcattta
    26161 gccacagttt cctgctgggt ggcgcagtgt tacgacacaa caccagtacg caactcaatg
    26221 gcgaaaacag catgttgcgg atcaatagcc tggcgatgcc ggtcaaaaac gaggtgtgtg
    26281 atacccgtac ctggctggaa cacaataaag gtttttgtaa cagccgacag ttgcacaaaa
    26341 ctatcgtcag cgacaaaggc cgcgcggtat ttaacggttt gatcaacgtc gcgcagcacg
    26401 ccatcaaaac ggatggtcag atgaccaaca ataatctgct gatgggcaaa ctggcggaag
    26461 tggatacgaa accgcagctg gaaatctatg cagatgatgt gaaatgcagc cacggcgcga
    26521 cggtggggcg tattgatgat gaacagatgt tctatctgcg ctcgcgcggg atcaatcagc
    26581 aggatgccca gcagatgatc atttacgcct ttgctgccga actgacggaa gcactgcgtg
    26641 atgaggggct taaacagcag gtgctggccc gaatcggtca acggctgcca ggaggtgcaa
    26701 gatgactttt tccgtcgaca aagtgcgggc cgactttccg gtgctttcgc gtgaggtaaa
    26761 cggtttgccg ctggcttatc tcgacagcgc cgccagtgcg cagaaaccga gccaggtgat
    26821 tgacgccgag gccgagtttt atcgtcatgg ctacgcggcg gtgcatcgcg gtattcatac
    26881 cttaagcgcc caggcgaccg agaaaatgga gaacgtgcgc aagcgggcat cgctgtttat
    26941 taatgcccgt tcggcggaag agctggtgtt cgtccgcggc acgacggaag ggatcaatct
    27001 ggtcgccaat agctggggaa acagcaacgt gcgggcgggc gataacatca tcataagtca
    27061 gatggagcac cacgctaaca ttgttccatg gcagatgctt tgcgcacgcg ttggcgcaga
    27121 gctgcgtgtg atcccgctca atcccgacgg tacgttgcaa ctggagatgc tacctaatct
    27181 gttcgatgag aaaactcgcc tgctggcaat tactcatgtc tccaacgtgc ttggcacaga
    27241 aaatccactg gcggaaatga tcacgcttgc gcaccagcat ggcgcaaaag tgctggtgga
    27301 tggcgctcag gcggtgatgc atcatccgga ggatgttcag gcgctggatt gcgactttta
    27361 cgtgttctcc gggcataaac tgtatggccc caccggaatt ggcattcttt atgtgaaaga
    27421 agccttgttg caggagatgc cgccgtggga agggggcggt tctatgatcg ccaccgtcag
    27481 cctgagtgaa ggcactacct ggaccaaagc accatggcgg tttgaagccg gtacacccaa
    27541 taccgggggc atcattggtc ttggcgcggc gctggagtat gtttcggcgc tggggcttaa
    27601 taacatagcc gagtatgaac agaatctgat gcactacgcg ctatcacagt tggaatctgt
    27661 accggatctc actctctatg gcccacaaaa caggcttggc gttattgctt ttaatctcgg
    27721 taaacaccac gcctatgatg ttggcagttt tctcgataat tacggcattg ctgtgcgtac
    27781 cggacatcac tgcgctatgc cattaatggc ctattacaac gtccctgcga tgtgtcgggc
    27841 gtcgctggcc atgtataaca cccatgaaga agtggatcgt ctggtgaccg gattgcaacg
    27901 tattcaccgt ctgctgggat aacagggagg cactatggct gtattgccgg ataaagaaaa
    27961 attgctgcgt aattttttac gctgcgccaa ctgggaagag aaatatctct acattattga
    28021 gctgggccag cgtctgccag aattacgcga cgaagacaga agcccacaaa atagcattca
    28081 gggctgccag agtcaggtgt ggattgtcat gcgccagaat gcgcagggaa ttattgaatt
    28141 gcagggcgac agcgatgcgg cgattgtgaa agggcttatt gcggtcgtct ttattctcta
    28201 cgatcagatg acgccgcagg atattgtcaa tttcgatgtg cgtccgtggt ttgaaaaaat
    28261 ggcgctcacc caacatctca ccccatctcg ttcacaaggt ctggaagcga tgattcgcgc
    28321 aattcgcgcc aaagccgctg cacttagcta aactaaaggg acagctttca tccggttggc
    28381 tcacattgcc ggatggtcaa tgtttacact gacctgcaca ggagtgtttc ggtttcaggt
    28441 gaacataagc gccatccgac gtttcagcgt gagtctccgg caaatacagg aggtttacaa
    28501 tgaaacgcgc gtctttgctt acactcacgc ttatcggcgc ttttagcgcc atccaggctg
    28561 cctgggcggt tgattatccg ctaccaccaa ccggaagccg actggttggg caaaatcaaa
    28621 cgtatacggt gcaggaaggg gataaaaacc ttcaggccat cgcccgacgt tttgatactg
    28681 cggcaatgtt gatccttgaa gccaataaca ctatcgcccc ggtgccaaaa cccggtacga
    28741 cgataactat tccttcgcaa ctgttattac ctgatgcgcc gcgtcagggg attatcgtta
    28801 accttgcaga gctgcgcctt tattattatc cgccgggaga aaatatcgtg caggtttatc
    28861 cgataggtat tggcttgcag gggctggaaa cgccggtgat ggaaacgcgt gtggggcaga
    28921 aaatccctaa cccaacctgg acgcctacgg caggcattcg tcaacgttcg ctggagcgtg
    28981 gcattaaatt accgccagtc gttcctgccg gaccaaataa cccgctagga cgttacgcac
    29041 tgcgactcgc gcatggtaat ggcgaatacc tcattcatgg caccagtgcg ccggacagcg
    29101 tcggtttgcg cgtcagttca gggtgtattc gcatgaatgc accggatatt aaagccttgt
    29161 tctccagcgt gcggacggga acgccggtga aagtgatcaa cgaaccggtg aaatattccg
    29221 tggagcctaa cgggatgcgt tatgttgaag tacatcgacc actatcggca gaagaacagc
    29281 agaacgttca gacaatgcca tacacactgc cagcaggctt tacgcaattt aaagacaata
    29341 aggctgtaga tcagaagtta gtcgataaag cgttgtatcg tcgggcaggg tatccggttg
    29401 cggtgagcag tggagcaact cccacagcca gcaatgcgcc ttcagtagag tcagcgcaga
    29461 atggtgaacc agagcaaggg aatatgttac gcgcgacgca gtagtggtaa atagcagaca
    29521 aaaaaaatgg cgcacaatgt gcgccatttt tcacttcaca ggtactatta cttgcggtat
    29581 ttagtagcca tgttgtccag acgctggtta gcacgagctg cgtcatcttt agcagcctga
    29641 acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt caactttagc gttcagagtc
    29701 tgaacgtcag aagacagctg atcgatttta gcgttgctgg agcaacctgc cagcagagta
    29761 gaacccagga ttaccgcgcc cagtaccagt ttagtagctt tcattattaa taccctctag
    29821 attgagttaa tctccatgta gcgttacaag tattacacaa agttttttat gttgagaata
    29881 tttttttgat ggga
//
LOCUS       DAZGBC010000049        29841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGBC010000049 DAZGBC010000000
VERSION     DAZGBC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.049"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..708
                     /gene="bcsQ"
                     /locus_tag="ACHK1J_002129"
     CDS             <1..708
                     /gene="bcsQ"
                     /locus_tag="ACHK1J_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO7911389.1"
                     /translation="TTITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWA
                     RAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKSSGR
                     YQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRI
                     GSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLAN
                     WCLLNYSGLKTPVGSKS"
     gene            705..3323
                     /gene="bcsA"
                     /locus_tag="ACHK1J_002130"
     CDS             705..3323
                     /gene="bcsA"
                     /locus_tag="ACHK1J_002130"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO7911390.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3334..5673
                     /gene="bcsB"
                     /locus_tag="ACHK1J_002131"
     CDS             3334..5673
                     /gene="bcsB"
                     /locus_tag="ACHK1J_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO7911391.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAIAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5680..6786
                     /gene="bcsZ"
                     /locus_tag="ACHK1J_002132"
     CDS             5680..6786
                     /gene="bcsZ"
                     /locus_tag="ACHK1J_002132"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO7911392.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6768..10241
                     /gene="bcsC"
                     /locus_tag="ACHK1J_002133"
     CDS             6768..10241
                     /gene="bcsC"
                     /locus_tag="ACHK1J_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO7911393.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLHQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10323..12311
                     /gene="hmsP"
                     /locus_tag="ACHK1J_002134"
     CDS             10323..12311
                     /gene="hmsP"
                     /locus_tag="ACHK1J_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO7911394.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12494..13780
                     /gene="dctA"
                     /locus_tag="ACHK1J_002135"
     CDS             12494..13780
                     /gene="dctA"
                     /locus_tag="ACHK1J_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO7911395.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14001..15497
                     /gene="yhjJ"
                     /locus_tag="ACHK1J_002136"
     CDS             14001..15497
                     /gene="yhjJ"
                     /locus_tag="ACHK1J_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7911396.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15593..16522)
                     /gene="kdgK"
                     /locus_tag="ACHK1J_002137"
     CDS             complement(15593..16522)
                     /gene="kdgK"
                     /locus_tag="ACHK1J_002137"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO7911397.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICAELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16754..17521
                     /gene="pdeH"
                     /locus_tag="ACHK1J_002138"
     CDS             16754..17521
                     /gene="pdeH"
                     /locus_tag="ACHK1J_002138"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO7911398.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17591..19651
                     /gene="yhjG"
                     /locus_tag="ACHK1J_002139"
     CDS             17591..19651
                     /gene="yhjG"
                     /locus_tag="ACHK1J_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7911399.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19833..21155)
                     /gene="yhjE"
                     /locus_tag="ACHK1J_002140"
     CDS             complement(19833..21155)
                     /gene="yhjE"
                     /locus_tag="ACHK1J_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911400.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21548..22561)
                     /gene="yhjD"
                     /locus_tag="ACHK1J_002141"
     CDS             complement(21548..22561)
                     /gene="yhjD"
                     /locus_tag="ACHK1J_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO7911401.1"
                     /translation="MTQENEIKRPTQDLEHEPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPIIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22610..23509)
                     /gene="rcdB"
                     /locus_tag="ACHK1J_002142"
     CDS             complement(22610..23509)
                     /gene="rcdB"
                     /locus_tag="ACHK1J_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7911402.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24029..24631
                     /gene="yhjB"
                     /locus_tag="ACHK1J_002143"
     CDS             24029..24631
                     /gene="yhjB"
                     /locus_tag="ACHK1J_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO7911403.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24682..26331)
                     /gene="treF"
                     /locus_tag="ACHK1J_002144"
     CDS             complement(24682..26331)
                     /gene="treF"
                     /locus_tag="ACHK1J_002144"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7911404.1"
                     /translation="MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            26736..28133
                     /gene="ccp"
                     /locus_tag="ACHK1J_002145"
     CDS             26736..28133
                     /gene="ccp"
                     /locus_tag="ACHK1J_002145"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO7911405.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            28344..29744
                     /gene="gadA"
                     /locus_tag="ACHK1J_002146"
     CDS             28344..29744
                     /gene="gadA"
                     /locus_tag="ACHK1J_002146"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO7911406.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT         7054 a         7534 c         8208 g         7045 t
ORIGIN      
        1 acaaccatca ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg
       61 gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta acgttgattt tacccaccgt
      121 cagggctggg ccagagctat gctggatggc caggactggc gtgacgctgg gttgcgctac
      181 acctcgcagc tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca
      241 cagcactggc aaactcggct gagcgatatt tgctccggct tacagcaact aaaatccagc
      301 gggcgttacc agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag
      361 ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc
      421 cgactgcatc agcaagcgct gccggatggc gcacatattt tgattaatga cttccgtatt
      481 ggcagtcagg ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattactg
      541 ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagta
      601 ggtgaatatc gcagtgatgc gctggccgct gaagagatac tgacgctggc gaactggtgc
      661 ctgttgaact attccgggct gaaaacgcca gtcgggagta aatcatgagt atcctgaccc
      721 ggtggttgct aatcccgccg gtcaacgcgc ggcttatcgg gcgttatcgc gattatcgtc
      781 gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga
      841 tttttattcc gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt
      901 atccgcatat caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa
      961 catgctggtt actgatcggt acatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat
     1021 tttcaggtct gcagaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc
     1081 gcgttagcca taaaacacag catcttgatg agaaaaaaga gctcggtcat ttgagtgccg
     1141 gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc gctgattctg gcgttaatct
     1201 gcgttactca gccgtttaac ccgctggcgc agtttatctt cctgatgctg ctgtgggggg
     1261 tagcgctgat cgtacggcgg atgccggggc gcttctcagc gctaatgttg attgtgctgt
     1321 cgctgactgt ttcttgccgt tatatctggt ggcgttacac ctctacgctg aactgggacg
     1381 atccggtcag cctggtgtgc gggcttattc tgctcttcgc cgaaacgtac gcgtggattg
     1441 tgctggtgct cggctacttc caggtagtat ggccgctgaa tcgtcagccg gtgccattgc
     1501 cgaaagatat gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc
     1561 tcaacgtggt gaaaaatacc atttacgcct cgctgggtat cgactggccg aaagacaaac
     1621 tgaacatctg gatcctcgat gacggcggca gggaagagtt tcgccagttt gcgcaaaacg
     1681 tgggggtgaa atatatcgcc cgcaccactc atgaacatgc gaaagcgggc aacatcaaca
     1741 atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt cgactgcgac cacgtaccaa
     1801 cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa agagaaacag ctggcgatga
     1861 tgcaaacccc acaccatttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc
     1921 gtaaaacacc gaacgaaggc acgctgttct atggtctggt gcaggatggc aacgatatgt
     1981 gggacgccac tttcttctgc ggttcctgtg cggtgattcg ccgtaagccg ctggatgaaa
     2041 ttggcggcat tgctgtcgaa accgtgactg aagatgcgca tacctctttg cgcctgcacc
     2101 gtcgtggcta cacctctgca tatatgcgta ttccgcaggc ggcggggctg gcgaccgaaa
     2161 gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg cgggatggtg caaatcttcc
     2221 gtctcgataa cccgctcacc ggtaaagggc tgaagtttgc tcagcggcta tgttacgtca
     2281 acgccatgtt ccacttcttg tcgggcattc cacggctgat cttcctgact gcgccgctgg
     2341 cgttcctgct gcttcatgcc tacatcatct atgcgccagc gttgatgatc gccctgttcg
     2401 tgctgccgca tatgatccat gccagcctga ccaactccaa gatccagggc aaatatcgcc
     2461 actctttctg gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc
     2521 tggtggcgct gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg
     2581 tggaagaaga gtacgtcgac tgggtgatct cgcggcccta catcttcctt gttctgctca
     2641 acctggtggg cgttgcggta ggcatctggc gctacttcta tggcccgcca accgagatgc
     2701 tcaccgtggt cgtcagtatg gtgtgggtat tctacaacct gattgttctt ggcggcgcag
     2761 ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc
     2821 ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg
     2881 acggtggttt ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga
     2941 atctgttgct taaacgcggt cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga
     3001 tgggtaatga agttgggctg aaattaatgc cgctcaccac ccagcaacat atcgattttg
     3061 tgcagtgtac gtttgcccgt gcggatacat gggcgctctg gcaggacagc tatccggaag
     3121 ataagccgct ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc
     3181 tggcggagtt tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt
     3241 cctgggttgt atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg
     3301 atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt ctggatttgt
     3361 gcagtggcta tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg
     3421 caaccactga tcaatgctga gccagctata gccgcccaga cggaacaaaa tccgcaggtg
     3481 gggcaagtga tgccgggcgt gcagggcgct gatgcgccag tcgtggcgca gaacggtcct
     3541 tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc cgccgggcag catggtgcta
     3601 cgtggcatta acccgaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg
     3661 aaggcgatgc tcaacctcga atacacgcca tcgccatcgt tactgcctgt ccagtcgcag
     3721 ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc cagtgaccaa agaacagttg
     3781 ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt ttattaccga cttcaaccgt
     3841 gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg
     3901 ctttggctgg atgttgggcg gagcagtgga ctggatttga cctatcagac cctgaatgtg
     3961 aagaatgacc tgtcacactt cccggtacca ttctttgacc cgcgtgataa ccgcaccaac
     4021 accttgccga tggtctttgc gggtgcgccg gatgttgggc tgcaacaagc atctgccatt
     4081 gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat
     4141 aaccaactgc cggatcgcaa tgccattgtc tttgcaacca acgacaaacg gccggacttc
     4201 ctgcgcgatc atccggcggt aaaagccccg gtgattgaga tgattaacca tccgcagaat
     4261 ccttacgtca aactgctggt ggtgtttggt cgtgacgaca aagacctgtt gcaggcagcg
     4321 aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa gcgtggtagt gaatgaagtg
     4381 aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact gggtacgtac cgatcgtccg
     4441 gtcacatttg gcgaactgaa aacctatgaa gaacagttac aatccagcgg tcttgagcca
     4501 gcagcgatta acgtttcgct aaacctgccg ccggatctct acctgatgcg cagtaccggc
     4561 attgatatgg atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg
     4621 gatatcagcc tgaataacca gttcctgcaa tccttcaacc tgagcagtaa acaggaggcg
     4681 aaccgcctgc tgcttcggat tccggtatta caaggtttgc tggatggcaa aacggatgtc
     4741 tctattccgg cgctgaaact gggcgcgacc aaccagctgc gcttcgactt tgagtatatg
     4801 aacccgatgc cgggcggttc ggtggataac tgtattacct tccagccggt gcagaatcat
     4861 gtggtgattg gtgacgattc caccatcgac ttctcgaagt attaccactt catcccgatg
     4921 ccggatctac gcgcctttgc taacgcgggc ttcccgttca gccggatggc ggatctgtcg
     4981 caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac agatggaaac gttgctgaat
     5041 actgttggtt ttatcggcgc acagacgggc ttcccggcga ttaatctgac ggtgaccgat
     5101 gatggcagca ccattcaggg caaagatgcc gacatcatga tcatcggtgg tatcccggac
     5161 aaactgaaag acgataagca gatcgacctg ttggtgcagg cgaccgaaag ctgggtgaaa
     5221 acgccgatgc gccagacccc gttccccggc attgtgccgg acgagagcga tcgcgcggca
     5281 gaaacccagt caacgctgac ctcttccggt gcgatggcgg cggtgattgg cttccagtcg
     5341 ccgtataacg accagcgcag cgtgattgcg ctgctggcag atagcccacg cggttatgaa
     5401 atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg
     5461 gtgatccgcg agtccggtat caacagccta cgtgttggcg acgtttatta cgtaggtcat
     5521 ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc atccgattct gctggcggtg
     5581 ctggcggcta tcagtgtgat attgctggca tgggtactgt ggcgtctgct gcgtattatt
     5641 agtcgtcgtc gtcttaaccc ggataacgag taattgaaga tgaatgtgtt gcgtagtgga
     5701 ctcgtgacga tgctgctgtt ggctgccttt agtgttcagg cagcctgtac ctggcctgcc
     5761 tgggagcagt ttaaaaagga ttacatcagt caggaagggc gcgtcatcga ccccagcgac
     5821 gcgcgcaaaa tcaccacctc cgaagggcaa agttacggca tgttctttgc cctggcggct
     5881 aacgaccgtg cagctttcga taatattctc gactggacgc agaacaatct cgctcagggt
     5941 tctttaaaag aacgtttgcc cgcctggctg tggggcaaga aagagaacag taagtgggaa
     6001 gtgctggaca gcaattcggc ctccgatggt gatgtctgga tggcctggtc gttgctggag
     6061 gcggggcgtt tgtggaaaga gcagcgttat accgacatcg gcagcgcgtt gctaaaacgt
     6121 attgcgcggg aggaagtggt gacggtgcct gggctgggtt ccatgttgtt accgggcaaa
     6181 gtgggttttg cagaggataa cagctggcgt tttaacccca gctacctgcc gccgacgctg
     6241 gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc tgcgcgaaac caatcaacgt
     6301 ttattgctgg aaaccgcccc gaaaggcttt tcgccagact gggtgcgcta tgagaaagac
     6361 aaaggctggc agctaaaagc cgaaaaaacc ttgatcagca gctacgacgc catccgcgtt
     6421 tacatgtggg taggcatgat gcctgacagc gatccgcaaa aagcgcggat gctcaaccgg
     6481 tttaaaccga tggcgacatt cactgagaaa aacggttatc cgccggaaaa agtggatgtg
     6541 gctacgggga aagcgcaggg taaaggaccg gtcggttttt ctgccgccat gctgcccttt
     6601 ttacaaaacc gtgatgcgca ggccgttcag cgccagcgcg tggccgataa ctttcccggc
     6661 agcgatgcct attacaacta tgtgctgacc ctgtttggac aaggctggga tcaacaccgt
     6721 ttccgcttct cgacaaaagg tgagttatta cctgactggg gccaggaatg cgcaaattca
     6781 cactaaacat attcacgctt tccctcggtc tggccgtcat gccgatggtc gaggcagcac
     6841 caaccgctca gcaacagttg ctggagcaag ttcggttagg cgaagcgacc catcgtgaag
     6901 acctggtgca gcagtcgttg tatcggctgg aacttattga tccgaataac ccggacgtcg
     6961 ttgccgcccg tttccgttct ttgttacgtc agggcgatat tgatggcgcg caaaaacagc
     7021 tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta taaatcgtcg cggactacga
     7081 tgctactttc cacgccggat ggtcgtcagg cactgcaaca ggcaagattg caggcgacta
     7141 ccggtcatgc ggaagaagct gtggcgagtt acaacaagct gttcaacggt gcgccgccgg
     7201 aaggtgacat tgctgtcgag tactggagta cggtggcgaa aattccggct cgccgtggcg
     7261 aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc gggcaatacg ggcctgcaaa
     7321 acaatctggc gctattgctg tttagtagcg atcgccgtga cgaaggtttt gccgtcctgg
     7381 aacagatggc gaaatcgaac gccgggcgcg aaggggcctc taaaatctgg tacgggcaga
     7441 ttaaagacat gcccgtcagc gatgccagcg tgcaggcgct gaaaaaatat ctttcgatct
     7501 ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact ggcagaacag caaaaacagc
     7561 tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc ggcggtggac tctggtatgg
     7621 cgggtaaagc cattcccgaa ctacaacagg cagtgcgggc gaacccgaaa gacagtgagg
     7681 ctctgggggc gctgggccag gcatattcac agaaaggcga tcgcgccaat gcagtggcga
     7741 atctggaaaa agccctcgca ctggacccgc acagcagcaa caacgacaaa tggaacagtc
     7801 tgctgaaagt aaaccgctac tggctggcaa tccagcaggg cgatgctgcg ctgaaagcca
     7861 ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg taatgtcgat aacaccgaca
     7921 gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg aaaagattat cccgccgccg
     7981 aacgttatta tcagcagacc ttgcgtatgg acagcggcaa cactaacgcc gtgcgcgggc
     8041 tggcaaatat ttaccgccag caatcgccag aaaaagctga agcgtttatc gcctcactct
     8101 ctgccagtca gcggcgtagc attgatgata tcgaacgcag cctgcaaaac gaccgtctgg
     8161 cacagcaggc agaggcactg gaaaaccagg gcaaatgggc gcaggcggca gcacttcagc
     8221 ggcaacgact ggcgctggac cccggcagcg tatggattac ttaccgactt tcgcaggatc
     8281 tctggcaggc cggacaacgc agccaggccg atacgttaat gcgcaatctg gcgcagcaga
     8341 agccgaacga cccggagcag gtttacgctt acgggctgta cctctctggt catgaccagg
     8401 acagagcggc gctggcgcat atcaacagcc tgccgcgtgc gcagtggagc agcaatattc
     8461 aggagctggt taatcgactg caaagcgatc aggtgctgga aaccgctaat cgcctgcgag
     8521 aaagcggcaa agaggcagaa gcggaagcga tgctgcacca gcaaccgcct tcctcgcgta
     8581 ttgacctcac gctggctgac tgggcgcaac aacgacgtga ttacaccgcc gcccgcgctg
     8641 cgtatcagaa tgtcctgacg cgggagccaa ctaacgccga tgccattctt ggtctgacgg
     8701 aagtggatat tgctgccggt gacaaagcgg cggcacgtag ccagctggcg aaactgcccg
     8761 ctaccgataa cgcctcgctg aacacacagc ggcgcgtggc gctggcacag gcgcagcttg
     8821 gcgataccgc agcggcgcag cagacgttta ataagttgat cccgcaggca aaatctcagc
     8881 caccgtcgat ggaaagcgcg atggtgctgc gcgacggtgc gaagtttgaa gcgcaggcgg
     8941 gcgatccaaa gcaggcgctg gaaacctaca aagacgccat ggtcgcatcc ggtgtgacta
     9001 cgacgcgtcc gcaggataac gacaccttta cccgactgac ccgtaacgac gagaaagatg
     9061 actggctgaa acgcggcgtg cgcagcgatg cggcggacct ctatcgccag caggatctta
     9121 acgtcaccct tgagcatgat tattggggat cgagcggcac cggtggttac tccgatctga
     9181 aagcgcacac taccatgttg caggtggacg cgccgtattc tgacgggcgg atgttctttc
     9241 gcagtgattt cgtcaatatg aacgtcggca gtttctccac taatgccgat ggtaaatggg
     9301 atgataactg gggcacctgt acattacagg actgtagcgg caaccgcagc cagtcggatt
     9361 ccggtgccag cgtggcggtc ggctggcgaa atgacgtctg gagctgggat atcggcacca
     9421 cgccgatggg cttcaacgtg gtggatgtgg tcggcggcat cagttacagc gatgatatcg
     9481 ggccgctggg ttacaccgtt aacgcccacc gtcggcccat ctccagttct ttgctggcct
     9541 ttggtgggca aaaagactcc ccgagcaata ccgggaaaaa atggggcggt gtgcgtgccg
     9601 acggcgtggg gctaagcctg agctacgata aaggtgaagc aaacggcgtc tgggcatcgc
     9661 ttagtggcga ccagttaacc ggtaaaaatg tcgaagataa ctggcgcgtg cgctggatga
     9721 cgggctatta ctataaggtc atcaaccaga acaatcgccg cgtcacaatc ggcctgaaca
     9781 acatgatctg gcattacgac aaagacctga gtggctactc actcggtcag ggcggttact
     9841 acagtccgca ggaatacctg tcgtttgcca taccggtgat gtggcgggag cgcacggaaa
     9901 actggtcgtg ggagctgggg gcgtctggct cgtggtcgca ttcgcgcacc aaaaccatgc
     9961 cgcggtatcc gctgatgaat ctgatcccga ctgactggca ggaagaagct gcgcggcaat
    10021 ccaacgatgg cggcagcagt cagggctttg gctatacggc gcgggcatta cttgagcgtc
    10081 gcgtcacatc caactggttt gtcggtacgg caattgatat ccagcaggcg aaagattacg
    10141 cgcccagcca tttcctgctc tatgtacgtt attccgccgc tggatggcag ggtgacatgg
    10201 atttaccgcc gcagccgctg ataccctacg ccgactggta agttttcaga tagcgcctct
    10261 cttaatgccg ctgcgatcgg gtatactcgg gcggcaatct gggatttccg gggggagaca
    10321 atttgcgcgt aagtcgctcg ttaacaatca agcagatggc aatggtggca gccgttgtcc
    10381 tggtgttcgt ttttattttt tgcaccgttt tgctgttcca tctggtccag cagaatcgct
    10441 ataacacggc tacgcaactg gaaagcattg ctcgctctgt ccgcgaaccc ttatcttcag
    10501 ccattttgaa aggcgatatt cccgaagcgg aagctattct tgccagcatt aaaccggcag
    10561 gcgtggtcag ccgtgccgat gtagtgctgc ctaaccagtt ccaggcgctg cgtaaaagtt
    10621 ttattccaga gcgcccggtg ccggtaatgg ttactcgcct gtttgagcta ccggttcaaa
    10681 tctcgctggg cgtctactcg ctcgaacgtc cggcaaatcc gcagccaatt gcctatctgg
    10741 tgctacaggc ggattccttc cgtatgtata agttcgtgat gagcaccctc tcaacgttag
    10801 tgaccattta cttacttttg tcgttaatat tgacggtggc gattagctgg tgcattaacc
    10861 gcctgatttt gcatccgtta cgcaatattg ctcgcgaact taacgccatc ccagcccagg
    10921 agcttgttgg tcaccaactg gcattaccgc gtctgcatca ggacgatgaa atcggtatgt
    10981 tggtgcgcag ttacaacctc aaccagcaat tgctgcagcg ccattatgaa gaacagaacg
    11041 aaaatgcgat gcgcttcccg gtgtcggatt tgccgaacaa agccttgctg atggagatgc
    11101 tggagcaggt tgtcgcgcgt aaacaaacca ccgcgctgat gatcatcacc tgtgaaactc
    11161 tgcgtgatac tgcgggcgtg ctgaaagagg cgcaacgaga aattctgctg ctgacgctgg
    11221 tggaaaaact caaatcggta ctgtcgccac gtatgatcct cgcgcagatt agcggttatg
    11281 actttgctgt cattgccaac ggtgtacagg aaccgtggca cgcaatcact ttgggtcagc
    11341 aagtgctcac tatcatgagc gagcgcctgc cgattgaacg tattcaactc cgtccgcact
    11401 gtagcattgg cgtggcgatg ttctacggcg atctcaccgc cgaacagctt tacagtcgcg
    11461 ctatttctgc ggcatttacc gctcgccata aaggcaagaa tcagattcag ttctttgatc
    11521 cgcagcagat ggaagctgcc cagaagcggt tgacggaaga gagcgatatc cttaatgcac
    11581 tggaaaatca tcagtttgct atttggttac agccacaggt cgagatgacc agcggtaaac
    11641 tggtcagtgc ggaagtgtta ctgcgtatcc agcaaccgga tggcagttgg gacctgccgg
    11701 atggcttaat cgatcgcatt gagtgctgtg ggctgatggt taccgtcggt cactgggtgc
    11761 tggaagagtc ctgtcgattg cttgcagcct ggcaagagcg cggcattatg ctgcccttgt
    11821 cggtaaacct ctctgcgctg caactgatgc acccgaatat ggtggcggat atgctggaac
    11881 tgttaacccg ctatcgcatc cagccgggaa cactgattct ggaagtgaca gaaagccgac
    11941 gtattgacga ccctcatgct gcggtggcaa tcctccgtcc gctgcgcaat gccggagttc
    12001 gggtggcgct ggatgatttc ggcatgggct acgcagggct gcgtcagctg cagcatatga
    12061 aatcgttgcc aatcgacgta ctgaaaatcg acaaaatgtt tgttgaaggc ttgccggaag
    12121 atagcagcat gattgctgca attatcatgc tggcgcagag cctgaactta caaatgattg
    12181 ccgaaggcgt ggagactgaa gcacaacgcg actggctggc aaaagcgggc gttggtattg
    12241 cccagggctt cctttttgct cgtccactcc ctattgaaat cttcgaagag agttacctgg
    12301 aagaaaagta gctaccccaa actgattaca aaactttaaa aagtgctggt ttgtgcgagc
    12361 cagctcaaac tttttaacct ttttgtttca attatgatcc aggtacattt ctgtgatgtt
    12421 gtctgggtgt tattttaagg ccgcaggtac cccataacct tacaagacct gtggttttac
    12481 taaaggacac cctatgaaaa cctctctctt taaaagcctt tactttcagg tcctgacagc
    12541 gatagccatt ggtattctcc ttggccattt ctatcctgaa ataggcgagc aaatgaaacc
    12601 gcttggcgac ggcttcgtta agctcattaa gatgatcatc gctcctgtca tcttttgtac
    12661 cgtcgtaacg ggcattgcgg gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt
    12721 cgcactgctt tactttgaaa ttgtcagtac catcgcgctg attattggtc ttatcatcgt
    12781 taacgtcgtg cagcctggtg ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc
    12841 ggtagcggtt tacgccgatc aggcgaaaga ccagggcatt gtcgccttca ttatggatgt
    12901 catcccggcg agcgtcattg gcgcatttgc cagcggtaac attctgcagg tgctgctgtt
    12961 tgccgtactg tttggttttg cgctccaccg tctgggcagc aaaggccaac tgatttttaa
    13021 cgtcatcgaa agtttctcgc aggtcatctt cggcatcatc aatatgatca tgcgtctggc
    13081 acctattggt gcgttcgggg caatggcgtt taccatcggt aaatacggcg tcggcacact
    13141 ggtgcaactg gggcagctga ttatctgttt ctacattacc tgtatcctgt ttgtggtgct
    13201 ggtattgggt tcaatcgcta aagcgactgg tttcagtatc ttcaaattta tccgctacat
    13261 ccgtgaagaa ctgctgattg tactggggac ttcatcttcc gagtcggcgc tgccgcgtat
    13321 gctcgacaag atggagaaac tcggctgccg taaatcggtg gtggggctgg tcatcccgac
    13381 aggctactcg tttaaccttg atggcacatc gatatacctg acaatggcgg cggtgtttat
    13441 cgcccaggcc actaacagtc agatggatat cgtccaccaa atcacgctgt taatcgtgtt
    13501 gctgctttct tctaaagggg cggcaggggt aacgggtagt ggctttatcg tgctggcggc
    13561 gacgctctct gcggtgggcc atttgccggt agcgggtctg gcgctgatcc tcggtatcga
    13621 ccgctttatg tcagaagctc gtgcgctgac taacctggtc ggtaacggcg tagcgaccat
    13681 tgtcgttgct aagtgggtga aagaactgga ccacaaaaaa ctggacgatg tgctgaataa
    13741 tcgtgcgccg gatggcaaaa cgcacgaatt atcctcttaa tctcatcact tatgcccgca
    13801 ctcccttccc cgagtgcggg tgtttgcgca taattgaccc ctgtgcctga acttaccacc
    13861 tgggtgagtt caggtttatt tcacttcttc cgtgacattt tcatgttctt gcggtctaac
    13921 acgaagtgtt tttaacgtca tttagactga ccgacaacgg gcagtttttt tattaccagg
    13981 atagttgatc aggggttcac atgcagggca caaaaattcg acttttagcg ggcggtttgc
    14041 tgatgatggc cactgctggc tatgtgcagg cagatgcgct ccagcctgat ccagcatggc
    14101 aacaggggac gctttccaac ggtttacagt ggcaagtgct gaccaccccc cagcgtccca
    14161 gcgatcgtgt tgaaattcgc ctgctggtta ataccggttc gctcgccgaa agtacacaac
    14221 agagcggtta cagtcacgcc atccctcgta ttgcgctaac gcaaagcggt ggccttgacg
    14281 cagcacaggc gcgttcattg tggcagcagg ggatcgaccc taaacgcccg atgccgccgg
    14341 taattgtctc ttatgacacc acgctgttta atctgagttt gcccaataac cgtaacgatt
    14401 tgctgaaaga ggcgctctct tatctggcaa atgccactgg caaactgact atcacgccag
    14461 aaaccatcaa ccacgcgctg caaagtcagg acatggtggc aacctggcct gccgatacta
    14521 aagagggctg gtggcgttat cgtctgaaag gatcaaccct gttaggtcac gatcctgccg
    14581 atccgctgaa acaacccgtt gaagcggaaa aaattaaaga tttctatcag aaatggtaca
    14641 ccccggatgc aatgacgctg ctggtggtgg gaaacgtgga tgcgcgctcg gtcgtcgacc
    14701 aaatcaacaa aacgtttggc gaactgaaag gcaaacgtga aacaccggct ccggtgccga
    14761 cgctttctcc gctgcgtgcg gaagcggtga gtattatgac tgacgcggtg cgtcaggacc
    14821 ggttatctat catgtgggat acgccgtggc agccgattcg tgaatcagcc gcactgctgc
    14881 gctactggcg tgcggacctg gcccgtgagg cgctgttctg gcatgttcag caagcgttaa
    14941 gcgccagtaa cagcaaagac atcggtcttg gatttgactg ccgtgtgctg tatctgcgtg
    15001 cgcagtgtgc catcaacatc gaatcaccaa acgacaagct gaacagcaac cttaatctgg
    15061 tggcgcgtga actggcgaag gttcgcgata aaggtctgcc ggaagaagag ttcaatgcgt
    15121 tagtggcgca aaagaaactg gagctgcaga aactgtttgc cgcctatgca cgagctgata
    15181 ccgatattct gatgggtcag cggatgcgtt cgttgcaaaa tcaggtcgtc gatatcgcgc
    15241 cggagcagta tcagaaactg cggcaggatt tccttaacag cctgactgtg gagatgttaa
    15301 atcaggatct gcgtcagcag ttgtcgaatg atatggcgtt aatactgctg cagccgaaag
    15361 gcgagccgga atttaacatg aaagcgttgc aggcggcctg ggatcaaatc atggccccat
    15421 ctaccgccgc tgcgaccacc tctgtcgcca cggatgacgt acatcctgaa gtgacggata
    15481 ttccacctgc acagtaatgc ctgatggtga tgagttagca caaccatcag gtatttatgg
    15541 atgagctgga tagaggggta acactttatc cagccttttg catatgctgc gtttacgctg
    15601 gcatcgcctc acgcgggata atcgcgccgc gatactgaat aacggtactt gcggtcaggt
    15661 gcccgcgttt cgctgcgttt tccgcactgc cgcccgtcaa acgtaccgcc agataaccgg
    15721 cactgaaaga gtcgccagct gcggtggtat cgatcacttt ttctttcggc agtttcaccg
    15781 ctggaacatc cactaaccct tcgccagcaa tagacaccag gcaagaatcc gccccgcgtt
    15841 tcaccaccac ttctttcacg cccgcgttat gggtgcgcgc aatgacgtct tccaccggct
    15901 gttgacccca cagcgcgtct tcatcgtcca gcgtcaggaa ggcgatatcc gtgcattcca
    15961 gcatttgttg gtacacctgc tgtgtctctt ctttgctggc ccacaggcgc ggacgatagt
    16021 tattgtcgaa aatcactttt ccgccgttgg cgcggcattc gcgcagcagg gaaagcagct
    16081 tttcgcggct ggtcgggctt aagatcgcca ggctaatccc gctcaggtag agataatcga
    16141 aattcgccag ctctgcacaa atcgccgcag actgctcgct ctccagccag aatttggcag
    16201 cggcttcgtt acgccagtag tagaacgtac gttcaccggt gctgtcggtt tcaatgtagt
    16261 aaaggcccgg cagacggttt tccatccgtt gggtcaggga agtatcaacg ttctcgccgt
    16321 gccaggcgtc cagcatttgc tggctaaaac tgtccgttcc cagcgccgtt acgtaatgaa
    16381 cggttaatgc cgcaggatcg acctgacggg cgatatagac ggaagtattc agtgtatcgc
    16441 cgccgaaacc gcgcttaacg tccgcgcctt tctcggaaag ctcaatcatg cattcgccaa
    16501 tcacggcaat ctttttggac atagtcgtga acctgatctg taaaaaatta gcgttagttt
    16561 gcgctgtggt ggtttgctgg tcaaccatat taaaacagtg ttccattatt tttttgagcc
    16621 agaacgtatt cctgaaagat tccgttgtgg agtgaggaaa tttaccccgg ccaggcggta
    16681 aagttctgcc cttacgcgcc gataatcttt gtcgagtccg ggcagcatca cttttaaaca
    16741 caggacatct ttgatgataa ggcaggttat ccagcgaata agcaaccctg aagcaagcat
    16801 cgagagcttg caggaacggc gtttttggtt gcagtgtgag cgtgcttaca cttggcagcc
    16861 gatctatcaa acatgcgggc ggttaatggc cgtggagcta ttaacggtgg tcacgcatcc
    16921 cttgaaccct tcgcaacgcc tgccgccgga tcgctatttt actgaaatca ccgtcagcca
    16981 tcggatggag gttgtgaaag agcagattga tttgctggcg caaaaagccg acttctttat
    17041 agagcacggc ctgctggcat cggtcaatat tgatggccct acgctcatcg ccctgcgtca
    17101 gcaaccaaaa atcctgcgcc aaattgagcg tcttccctgg ctgcgtttcg aactggtgga
    17161 gcatatccgt ctgccgaaag attcaacctt tgcctcgatg tgtgaatttg gcccgctgtg
    17221 gctggatgat tttggtaccg ggatggcaaa tttctctgca ctaagtgaag tgcgttatga
    17281 ctacatcaaa atcgcgcgag aactgtttgt gatgctgcgt cagtcgccgg aaggccgcac
    17341 actcttttct cagcttttac atctaatgaa tcgctattgt cgcggggtga ttgtcgaggg
    17401 cgtagaaaca ccggaagagt ggcgtgatgt tcagaactcg cccgcattcg ccgcacaagg
    17461 ctggtttctt tcacgcccgg caccgataga aacgctgaat acggcggttc tggcgctata
    17521 agctgcctca ttttccgcct ggctggacta tctttaggac tggcacagga agaatatgag
    17581 gaagcgaacg atgagcaagg caggcaaaat aaccgctgcg atttcagggg ctttcttgtt
    17641 gttgattgtc gtggcgatca ttttgattgc aacttttgac tggaatcgac tcaaaccgac
    17701 catcaaccag aaagtctctg cggagttgaa tcgtccgttc gctatccgtg gcgatctggg
    17761 cgtggtgtgg gagcggcaaa aacaagaaac tggctggcgc agctgggtgc cgtggcccca
    17821 tgtacatgcg gaagacatca ttcttggcaa tccaccggat attcccgaag tcacgatggt
    17881 gcatttgcca cgcgtagagg caacgctggc accgttggcg ctgctgacca aaacggtctg
    17941 gctaccgtgg atcaaactgg aaaagcccga cgcacgcctg attcgcctct ctgaaaagaa
    18001 caataactgg acgtttaatc ttgccaacga tgacaacaaa gacgcgaatg caaagccgtc
    18061 ggcatggtcg tttcggctgg ataatattct tttcgatcaa gggcggatcg ccattgatga
    18121 caaagtaagc aaagcggatc tggaaatttt tgtcgatccg ttaggcaagc cactgccgtt
    18181 cagcgaagtt actggatcga aaggtaaagc ggataaagaa aaggtgggcg attacgtttt
    18241 tggcctgaag gcgcagggac gttataacgg tgaaccgctc actggtacgg gaaaaatagg
    18301 cggtatgctg gcgctgcgtg gcgaagggac gccgtttccg gtacaggctg atttccgttc
    18361 aggtaatacc cgtgttgctt ttgatggcgt cgtgaatgac ccaatgaaga tgggcggtgt
    18421 cgatttacgg cttaaatttt ctggcgattc actgggtgat ctctatgaac tgacgggcgt
    18481 tctgctgccc gataccccgc cgtttgaaac ggatggtcgg ctggtagcga aaatcgacac
    18541 tgaaaaatcg tcggtctttg attatcgcgg ttttaatggg cgcattggcg atagcgatat
    18601 ccacggttct ctggtctaca ccaccggaaa gccacgacca aaactggaag gtgatgtcga
    18661 gtcgcggcaa ttgcggctgg cggacctggg accgttgatt ggcgttgatt ccgggaaagg
    18721 ggctgaaaag tcgaaacggt ctgaacagaa gaagggcgaa aaaagcgttc agcctgcggg
    18781 taaagtgctg ccttatgacc gcttcgaaac cgataaatgg gacgtcatgg atgccgatgt
    18841 tcgtttcaaa ggccgacgta tcgagcatgg cagtagcctg ccgattagcg atctttctac
    18901 tcatatcatc ctcaaaaatg ctgacctgcg cctgcaaccg ctgaaatttg gcatggcggg
    18961 tggcagcatt gcggcgaata ttcatctgga aggcgataaa aagccgatgc aggggcgggc
    19021 agatattcag gctcgtcgac tgaaactgaa agaactgatg cccgatgtgg aactgatgca
    19081 gaagacgctg ggggaaatga acggtgacgc ggaactacgc ggtagcggta actcggtggc
    19141 ggcgctttta ggcaacagta acggcaacct gaaactgttg atgaatgacg ggctggtgag
    19201 ccgcaacctg atggagattg ttgggctgaa tgtcggcaac tacattgtcg gtgcgatatt
    19261 tggtgacgat gaggtgcggg tgaactgcgc ggcggcgaat ctgaatattg ccaacggcgt
    19321 ggcacgcccg cagatttttg ctttcgatac tgagaacgcg ttgattaacg ttaccggcac
    19381 ggcaagtttt gcttcggaac agctggattt gactattgat ccggagagta aagggattcg
    19441 gattatcaca ctgcgttcgc cgctgtatgt gcgtgggacg tttaaaaatc ctcaggctgg
    19501 ggtgaaagcc gggccgttga ttgcccgtgg tgctgttgct gcggcactgg caacgctggt
    19561 aacaccggcg gcggcgttac tggcgctgat ctcaccttcc gaaggggagg ctaatcagtg
    19621 tcggacgatt ttgtcgcaga tgaagaagtg atgataattg ccggatgtct gacatccggc
    19681 aattaaacag atttagattg ttggcaaagt gtgctttgtt catgccggat gcggcgttaa
    19741 cgccttatcc ggcctacaaa tccttgttaa ttcaatatat tgtatggaaa ttcaggcctg
    19801 ataagcatag cgcatcaggt ttttcactct tattacaacg actgatgtcg cgtctcatgg
    19861 gtcagcagca gggcgattaa cgtcaagcca gccatcgccg ccagatataa ccccaccgca
    19921 cctagcccgt agttagtctg caaccaggct gcgatatatg gcgcaacgga agccccgaga
    19981 atcgacgcta cgttgtaaga gaacgatgct ccggtgtaac gcacttctgt cggaaacagc
    20041 tctggtaaca gcgcacccat tggcccgaag gtcagcccca tcagacttaa ccccagcagc
    20101 aggaaggcaa aaaccagaat cgggttgcca gaaccgagca gcgggttaaa ggcgaacagc
    20161 gcgaacagga tgatcagcgt ggtgataatt accatgcttt tacggcgacc aaaggcatca
    20221 gccagtaatc cggcgactgg caccatcacg ccaaaaccaa taactgccat catcagcatc
    20281 cacaacactt cgttgcgcgg caggccaagc ccaactggcg cggcggcggt actaaaggtc
    20341 atagagtaga ccgtcatgat gtaaaatagc gtataggttg ccagcataat gaacgtaccc
    20401 agtacggtta cgcgaacatg tttggtcagc agcgtaccca gcgggatctt cacctgtttt
    20461 ttcgctttag cgactttctc aaacaccggc gactcatgca gcgacacgcg aacatacagg
    20521 ccgataatga ccagcaccgc cgagaagata aatggcacac gccagcccca gctcataaac
    20581 tgctcgtcgg tcagtagcca ggaaagcagc aagaaagtgc cgttggcaaa gaagaagcca
    20641 atcggtgcgc ccagctgtgg gaaggagcca tacagagcac gtttgcgcgg tggggcgttt
    20701 tcagtcgcca gcagcgccgc accgccccat tcaccgccta agccaagacc ctgaccaaat
    20761 cgagccagcg ccagcagcag cggggcgaaa ataccaatcg tggcatagcc cggcagcaga
    20821 ccaatcacca cggtcgaaat ccccatcgtt agcaacgagg cgaccagcgt cgctttacgc
    20881 ccaacgcgat cgccaaaatg accaaatacg gcagagccaa tggggcgcgc gacgaaggcg
    20941 atggcgaagg tggcgagcga ctgtagcgtt gctgccgttg gatcgccctg cgggaagaag
    21001 atatgcggaa acacaataac ggccgcagtg gcgtaaatgt aaaagtcgaa gaactcaatg
    21061 gccgtgccaa tgagagaggc gacaaggact ttattacgcg agttgatcgg cgtgtattct
    21121 tgctcgtggt cgagtgttgt ggctgttgct tgcataatct tttcttattt tttggctaac
    21181 gaatagccat attacgcaca gcaaaagtga tatttcaatg tgtaacaaat cgcggaaatg
    21241 gtcaaaaata aggcaaaaag gggataatgt ttaggccagt gatttttctt ctatgaaatg
    21301 cttcacataa ttttataaat ggtggataaa actggtttca ggttaaataa aagttaccaa
    21361 ctggatttat atgctggggt tatgagttgg catgaaattg tttgctccca aatagatggc
    21421 tgaagaggct ctggggaaga gtttcaagag gttatgtgcg cattatagcc ggatgcgatg
    21481 ctggcgcatc ttatccggcc tacggggagc caggccgaat aaattcgtac gccgtatccg
    21541 gcctatttta aggctgtgtt ttccccggca ttcgcgggtc gtctttatat tcggcggtgg
    21601 caatccacgc cgcgcaaaac agcgtcaaac gggcgaagaa gtagaaaaat gccatcagcc
    21661 ccagcacgga accaaatgct gcgccagagg gggatttcat cagcgatggt agggtgtagg
    21721 tcatcacgat tttaatcact tcaaaaccaa tagcagccag aaatgttccg cgaatcaggg
    21781 cttttttgcg cggacggtgg cgcggtaacc gccagaagat ccagaagaaa agcagatagt
    21841 tggcgaagat ggaaatcgcc aggccaatca accgccacgt cggtttcagc cactcaatgc
    21901 tgttcaggtg cagggcgcta ataatcattt gctgcgccga accggcaacc gaggtgatcg
    21961 aaagcgtcac aatcagcgca atcaacaaac caatcagcga aataaaatca cgcagatatt
    22021 ttacccagaa cttctcctga tcttgcggcg agcgttccca gacatcgcgc gactgggcgc
    22081 gaatcgcttc acgcaggtta cccatccagt tgatgccgga ataaagcgcc accgccaggc
    22141 cgacaagccc tacagtcgta cgctgctgaa cggcggtgtt gatggtgttt ttcaacgtag
    22201 cggctagcgt cggatcgctg atgttttgca gaattttgtc gaagatatcc tgtagcaaca
    22261 tcggatggga ggccagcaca aagccccctg cggcaaacga caccatcaaa atcgggatca
    22321 tcgacaaaaa tgagaaatag gtgatagccg caccaaactg gttacccagc cgatcgttaa
    22381 agcgttctgt cgcgcgaata aggtgcgcaa taatcggttg gcgttggact ttttcggcag
    22441 tggtggtgac ggtttccagc gcctggctaa ctttgccgcc tttctcgcta ttatccaacg
    22501 gcttaatcgg ctcgtgctcc agatcctggg tgggacgttt gatctcgttt tcctgcgtca
    22561 tccgctcttt tgtccttctg tcgtggtcat tcttaaaaag tatagtcggt cagtccaggt
    22621 actctttcat tacgccagcc agccactgca taaacaggtt tacacgccgg gaaagctccc
    22681 gacgctgcgg ataaaccagc gaaagggaga gcggctcggc acggtagcct ggcaacactt
    22741 caataagccg cccggcacgc agggcttcgc gcacggcaat gcgcgggatc tgaataatcc
    22801 ccagaccggc aagaccggcg gcgagatagt tttcgctact gtttaccgtc aacatgccgc
    22861 cagacttaaa ccactggacg ccattaacgc tggcaacctc aaaacctaac ggatgtacac
    22921 ccaggtgcgg tgtataacgc actattgcgt gtgaagtcag atcatcgggg ctttgcggat
    22981 aaccaaagcg cgtcagatag tgcggactgg cacagttgac catggtcagt ttgccgaggg
    23041 gacgggcgat aacgccatct tccggtaacg cgccagtgcg tatcacgcaa tcaaaaccat
    23101 catgaagaat atctaccgga cggtcatggc tactcagttc cagctcaatt cccggatgca
    23161 gatagagaaa ttccgacagg cgcggcagta acaggctttt cgcgattcct ggcgggatgt
    23221 cgatgcgtaa tttaccgctg atactggtgg catcctgttg aaacagaccg tccagttcgc
    23281 tgagattact caacacatct tttgctcgtt gataataggt catgccttct ggcgtgagtt
    23341 tgacccgtcg cgtggtgcgc tggagaagct gggtgccaag ttgatgttcc agtgcctgta
    23401 tctggcgcga aacacttcct tttggcaaag caaagaaatc cgccgcgcgg gaaaaacttt
    23461 ccagctccgc gactttgatg aacaactgca ttgcgtgaat tttatccatt tctttgaccg
    23521 attgttgttc acagcgaaac agtaacgcgc atatcgcatt gtttattgtt ttttctaaca
    23581 tctaatgccg ggtttcacaa tgttgtcatg acgaatgtgg cgatttctca gcaggaaaag
    23641 ggcggaatag ctttctgaga aaggtgaatc cccttaaaca gagcgtattt ttcagcgatt
    23701 ccctttaaag tgaatagagg acgtattggc tatttgtgct tatttttgtc tcattgccat
    23761 ttgttatttt ggcttgtggt cgcggaattt catttttaat tgcatgcgta ctaaccatta
    23821 atataaggaa atcctcaaaa ttgttttaaa tagtgccctc gacatgtaat ttcaggcgtt
    23881 tagctgtaac tatttatatc ggtaactatt ttctacgcgc catctctgta gaatggccac
    23941 cgccaaatgg cgaattctta ttttttctcg cgggtaatac ccttccacta cttaagtctt
    24001 tttaacgtat tcaggtggag attcagttat gcaaatagtc atgtttgaca ggcagtcaat
    24061 atttattcat ggaatgaaaa tcagtttaca gcagcgtatt ccaggagtga gtattcaggg
    24121 ggccagtcag gcagacgagc tatggcaaaa gctggaaagt taccctgaag ccttagttat
    24181 gctcgatggc gatcaggatg gtgagttttg ctactggttg ctgcaaaaaa ccgtggtgca
    24241 atttcctgag gttaaggtgt taattacggc gacggattgc aataaacggt ggttacagga
    24301 agttattcat tttaatgtgc tggccattgt gcctcgtgat tcaaccgtcg agacatttgc
    24361 gttggcggta aatagtgcgg cgatggggat gatgtttctg cctggagact ggcggacgac
    24421 gccggaaaag gacattaaag atctcaaatc gttaagcgcc cgccagcggg agattttaac
    24481 catgttagcg gcgggcgaat caaataagga gatcggcagg gcgctgaata tcagtaccgg
    24541 aacggtaaaa gcgcatctgg aatccctgta tcgccgtctg gaagtgaaaa atcgcaccca
    24601 ggcggcaatg atgttaaata tctcctcctg aagagaataa ttttacagcc agcaggaagt
    24661 tagcggctgg ctgtaaaaat attatggttc gccgtacaaa ccaattaaac ggcgtaccac
    24721 accgttagtc cagccaaacc catcctgcaa cggatactcg ccaccgccgc cttcgcgggg
    24781 aacaccatcg gcaatatggt atttctcgat cagtttgtgc tgttccagat agaactgatt
    24841 caccgtcttc agccagctgc gcgcgatttc atcacccaga aggtcatctc catacatttt
    24901 aaatccctga atcgccatcc attgtaacgg tgcccagccg ttgggtttat cccactgttc
    24961 accggtttcg tactcgcttg ccagaatccc gccaggtgtc agtaaccgac tgcgcacggc
    25021 gtttgccaga cgatcggcct gttcatggtt cgccataccg acatagagtg gcacaatggc
    25081 ggcagcggaa aacagcgcca gttgttcgcg tcgccagtcg taatcgcggt agatgccgtt
    25141 ttcatcatcc cagaggtaac ggtttaccgc atcgcgacgg gcactggcct tctggcggaa
    25201 cagtgcttct gtctctttct cacctttcag cgccgagatg ttggcgatgg cgctctccag
    25261 tttaaacagg aaggcattca gatcgatggg gatgaactgg gtggtacgaa tgctcgccag
    25321 acgaccagta tcacgcagcc aacgggaaga gtaatcccaa ccggaggccg cccccgcgcg
    25381 taaatcgcgg tacacctcgt tgggcgggcg accagaatgt ttcgcggttt caacgtcctc
    25441 aagccaggat tcgtcacgcg gcgtgtcgcg atcgtcccag taacggttga gcagcgatcc
    25501 gtccggcatc cgcacaacat ggcgataggc ctgattaggg atcaacgatt ctgcaccgtc
    25561 catccagaag gcatattcca ttttcaggtg gtcgagatag cggcgtgcac cgcgtacacc
    25621 atcttcttca aacaactcca ccatcagcgc aaaaaccggt ggttgcgagc ggctcaaata
    25681 ataggtgcgg ttgccgttgg ggatgtgacc gtagttttcg atcatccagg cgaagttatc
    25741 ggccatgcat ttcagcaaat cttcccgacc actttccgcc agccccagca tggtgaaata
    25801 ggaatcccaa tagtaggttt cgctaaaacg gccgcccggg acaatatatg actgcggcag
    25861 cgccagcaga gaagaccacg gaatgtgatc ctgtggttcg cgggttagca ccggccacag
    25921 ctggtcgata tgctctttca gggaattttg cgggtccgat acatactcgc tggagtagac
    25981 ctccggcagc cagaagtggt tttcaacaaa cttgcgcaag tcaaaatcac gatgacggcg
    26041 cactttacgg tagcggatta aaatatccag cgggtccatt ttaggtgcgc agtcgggaaa
    26101 ggttttactg tcggggaaaa ttttcgccga ctgaacatgc tcgaacagtt cgagatagcg
    26161 atcggcaggc gtcagcgcat cagaggcggg cagcccttca atcatctcgg gttccggttc
    26221 tgcctcgatc atctcatcca gttttaattc atacgggtcc agttcatagc agagatcgat
    26281 ttcgatcatc agttcgtctg gattagggtt ttgaattttc tgattgagca taaggatcca
    26341 acctccgaaa ttcgtcgcta attatcgggc ttttctttaa gggtagtcat tcgctccgcc
    26401 tggtgggcga caaagcgtgc ggtggatcac gtttattaac ctgtcaaaaa atataacgca
    26461 ctaatatcca tggaatttat aaaaatacag atttggtgtt gtttttcatt ttcagaacat
    26521 tcaatcctgc tctcgccgca atcatgatat gccattcctg atctgggtaa taggcacagg
    26581 ctatcttatt gatagtttat attcatgtaa ttgattgatt cctatcaaaa agtgtatctc
    26641 cctgtttttt atgatggctt tgtctcgcag agacgtttgc agcattctgg aaaattttct
    26701 gggtcacaat aactcaccac cgtgagggaa gaataatgaa aatggtctca cgtattaccg
    26761 cgatcggcct ggctggcgtc gcgatttgct atttagggtt atctggttat gtgtggtacc
    26821 acgataataa acgcagtaaa caggccgatg ttcaggcatc tgctgtcagt gaaaataata
    26881 aggttttagg ctttctccgc gaaaaaggat gcgactattg ccacacgcct tcggcagaat
    26941 tacccgccta ttattatatt cctggcgcga aacagttgat ggattacgac attaagcttg
    27001 gatataaatc ttttaacctc gaggccgtgc gtgcggcact gctggctgat aaacccgttt
    27061 cgcaaagcga tctgaataag attgaatggg tgatgcagta tgaaactatg ccaccaacgc
    27121 gttataccgc gctacactgg gcgggtaagg tgagtgatga agagcgggcg gaaatactgg
    27181 cctggattgc aaaacagcgc gcggaatatt acgccagcaa tgatactgct ccggagcatc
    27241 gcaatgaacc ggtgcagccc atcccgcaaa aactgcctac cgatgcgcaa aaagtggcgt
    27301 tgggctttgc gctgtatcac gatccccgtt tatcggctga tagcaccatt tcatgcgctc
    27361 attgccatgc gttgaatgcg gggggcgtcg atggcagaaa aacatcgatt ggtgttggtg
    27421 gcgcagttgg gccgattaac gcgccgacgg tatttaactc agtatttaac gttgagcagt
    27481 tctgggatgg tcgtgcggca acattgcagg atcaggctgg tggaccgccg ttgaacccga
    27541 ttgaaatggc gtcgaaatcc tgggacgaaa ttattgctaa gctggaaaaa gatccgcagc
    27601 ttaaagcgca gttcctcgaa gtctatccgc aaggtttcag tggcgaaaat attactgatg
    27661 ccattgctga atttgagaaa acattaatta cgccggattc cccatttgat aaatggttgc
    27721 gtggagatga gaatgcgctg acggcgcaac agaaaaaagg ctatcaatta tttaaagata
    27781 ataaatgtgc aacttgtcat ggtggaatta ttctcggcgg acgttccttc gaaccgttgg
    27841 ggctgaaaaa agactttaac tttggggaaa ttacggcggc ggatattggt cgtatgaatg
    27901 tgactaaaga agagcgtgat aaattgcgtc agaaagtacc cggtttacgt aacgttgctt
    27961 taacggcacc gtacttccat cgcggtgacg tgccgacgct ggacggggcg gtgaaactga
    28021 tgctgcgcta tcaggtaggc aaagagctgc cgcaggagga tgtggatgat atcgtagctt
    28081 tcctgcacag tctgaacggg gtttacacgc cgtatatgca ggataaacaa taattaattt
    28141 gatcgcccga acaggaatgt ttgggcgatt tttattacga taataaagtc tgtttttaat
    28201 attatcatgt taaatgttta tattataaaa gatcattttt ctgcttagga ttttgttatt
    28261 taaattaagc ctgtaatgcc ttgcttccat tgcggataaa tcctactttt ttattgcctt
    28321 caaataaatt taaggagttc gaaatggacc agaagctgtt aacggatttc cgctcagaac
    28381 tactcgattc acgttttggc gcaaaggcca tttctactat cgcggagtca aaacgatttc
    28441 cgctgcacga aatgcgcgat gatgtcgcat tccagattat caatgatgaa ttatatcttg
    28501 atggcaacgc tcgtcagaac ctggccactt tctgccagac ctgggacgac gaaaacgtcc
    28561 ataaattgat ggatttgtcg atcaataaaa actggatcga caaagaagaa tatccgcaat
    28621 ccgcagccat cgacctgcgt tgcgtaaaca tggttgccga tctgtggcat gcgcctgcgc
    28681 cgaaaaatgg tcaggccgtt ggcaccaaca ccattggttc ttccgaggcc tgtatgctcg
    28741 gcgggatggc gatgaaatgg cgttggcgca agcgtatgga agctgcaggc aaaccaacgg
    28801 ataaaccaaa cctggtgtgc ggtccggtac aaatctgctg gcataaattc gcccgctact
    28861 gggatgtgga gctgcgtgag atccctatgc gccccggtca gttgtttatg gatccgaaac
    28921 gcatgattga agcctgcgac gaaaatacca tcggcgtggt gccgactttc ggcgtgacct
    28981 acaccggtaa ctatgagttc ccgcaaccgc tgcacgatgc gctggataaa ttccaggccg
    29041 acaccggtat cgacatcgac atgcacatcg acgccgccag cggtggcttc ctggcaccgt
    29101 tcgtcgcccc ggatatcgtc tgggacttcc gcctgccgcg tgtgaaatcg atcagtgctt
    29161 caggccataa attcggtctg gctccgctgg gctgcggctg ggttatctgg cgtgatgaag
    29221 aagcgctgcc gcaggaactg gtgttcaacg ttgactacct cggtggtcag attggtactt
    29281 tcgccatcaa cttctcccgc ccggcgggtc aggtaattgc acagtactat gaattcctgc
    29341 gcctcggtcg tgaaggctat accaaagtac agaacgcctc ttaccaggtt gccgcttatc
    29401 tggcggatga aatcgccaaa ctggggccgt atgagttcat ctgtaccggt cgcccggacg
    29461 aaggcattcc ggcggtttgc ttcaaactca aagatggtga agatccggga tacaccctgt
    29521 acgacctctc tgaacgtctg cgtctgcgcg gctggcaggt tccggccttc actctcggcg
    29581 gtgaagccac cgacatcgtg gtgatgcgca ttatgtgtcg tcgtggcttc gaaatggact
    29641 ttgctgaact gttgctggaa gactacaaag cctccctgaa atatctcagc gatcacccga
    29701 aactgcaggg tattgcccag cagaacagct ttaaacacac ctgataacat aacgttgtaa
    29761 aaaccgaatg cccagccttt aaaaaaacag ctgggcattc ggttgcttat taatcgcaat
    29821 aatatattgg ctgtctattc a
//
LOCUS       DAZGBC010000050        29618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGBC010000050 DAZGBC010000000
VERSION     DAZGBC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.050"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..3884
                     /gene="yfaL"
                     /locus_tag="ACHK1J_002147"
     CDS             132..3884
                     /gene="yfaL"
                     /locus_tag="ACHK1J_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO7911407.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDTTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIELRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFEHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFETGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALDASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     TGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSASDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIEVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFRGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4025..4747)
                     /gene="ubiG"
                     /locus_tag="ACHK1J_002148"
     CDS             complement(4025..4747)
                     /gene="ubiG"
                     /locus_tag="ACHK1J_002148"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO7911408.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4894..7521
                     /gene="gyrA"
                     /locus_tag="ACHK1J_002149"
     CDS             4894..7521
                     /gene="gyrA"
                     /locus_tag="ACHK1J_002149"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO7911409.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7670..9358
                     /gene="yfaA"
                     /locus_tag="ACHK1J_002150"
     CDS             7670..9358
                     /gene="yfaA"
                     /locus_tag="ACHK1J_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO7911410.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNNETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DEEQDTEATAIASDLLSGKKRWQVSFGLEERTAGKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFLALNDESASVDNSFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9355..9978
                     /gene="yfaT"
                     /locus_tag="ACHK1J_002151"
                     /pseudo
     CDS             9355..9978
                     /gene="yfaT"
                     /locus_tag="ACHK1J_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
     gene            10002..14516
                     /locus_tag="ACHK1J_002152"
     CDS             10002..14516
                     /locus_tag="ACHK1J_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO7911411.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLDGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWITL
                     QRLNDTQYEARVPVNSTFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSLLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEAPRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLTHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14517..16166
                     /gene="yfaQ"
                     /locus_tag="ACHK1J_002153"
     CDS             14517..16166
                     /gene="yfaQ"
                     /locus_tag="ACHK1J_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO7911412.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLNEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGDRYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16171..16947
                     /gene="yfaP"
                     /locus_tag="ACHK1J_002154"
     CDS             16171..16947
                     /gene="yfaP"
                     /locus_tag="ACHK1J_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO7911413.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIHGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17222..20071
                     /gene="rcsC"
                     /locus_tag="ACHK1J_002155"
     CDS             17222..20071
                     /gene="rcsC"
                     /locus_tag="ACHK1J_002155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO7911414.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20271..20921)
                     /gene="rcsB"
                     /locus_tag="ACHK1J_002156"
     CDS             complement(20271..20921)
                     /gene="rcsB"
                     /locus_tag="ACHK1J_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO7911415.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20939..22255)
                     /locus_tag="ACHK1J_002157"
     CDS             complement(20939..22255)
                     /locus_tag="ACHK1J_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7911416.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKAYNIFLKSNS"
     gene            complement(22289..22543)
                     /gene="xisR"
                     /locus_tag="ACHK1J_002158"
     CDS             complement(22289..22543)
                     /gene="xisR"
                     /locus_tag="ACHK1J_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7911417.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(22551..22697)
                     /locus_tag="ACHK1J_002159"
     CDS             complement(22551..22697)
                     /locus_tag="ACHK1J_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911418.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(22718..22960)
                     /locus_tag="ACHK1J_002160"
     CDS             complement(22718..22960)
                     /locus_tag="ACHK1J_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911419.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKAWAPAQPDELA"
     gene            complement(22953..23855)
                     /locus_tag="ACHK1J_002161"
     CDS             complement(22953..23855)
                     /locus_tag="ACHK1J_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911420.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(23852..24076)
                     /locus_tag="ACHK1J_002162"
     CDS             complement(23852..24076)
                     /locus_tag="ACHK1J_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911421.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(24093..24455)
                     /locus_tag="ACHK1J_002163"
     CDS             complement(24093..24455)
                     /locus_tag="ACHK1J_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO7911422.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYENLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(24448..25314)
                     /locus_tag="ACHK1J_002164"
     CDS             complement(24448..25314)
                     /locus_tag="ACHK1J_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO7911423.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(25338..25922)
                     /locus_tag="ACHK1J_002165"
     CDS             complement(25338..25922)
                     /locus_tag="ACHK1J_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911424.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(25909..26469)
                     /locus_tag="ACHK1J_002166"
     CDS             complement(25909..26469)
                     /locus_tag="ACHK1J_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911425.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(26877..27239)
                     /locus_tag="ACHK1J_002167"
     CDS             complement(26877..27239)
                     /locus_tag="ACHK1J_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911426.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            complement(27414..27614)
                     /locus_tag="ACHK1J_002168"
     CDS             complement(27414..27614)
                     /locus_tag="ACHK1J_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911427.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(27611..27802)
                     /locus_tag="ACHK1J_002169"
     CDS             complement(27611..27802)
                     /locus_tag="ACHK1J_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911428.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(27804..28181)
                     /locus_tag="ACHK1J_002170"
     CDS             complement(27804..28181)
                     /locus_tag="ACHK1J_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7911429.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(28267..28926)
                     /locus_tag="ACHK1J_002171"
     CDS             complement(28267..28926)
                     /locus_tag="ACHK1J_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7911430.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            29266..29559
                     /locus_tag="ACHK1J_002172"
     CDS             29266..29559
                     /locus_tag="ACHK1J_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911431.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT         7120 a         7147 c         7744 g         7607 t
ORIGIN      
        1 actgatacta ttcattaaat aataacaaaa tagctattgc atcgggttgg acaatttaca
       61 aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata taaaatgatg
      121 gtcagattaa tatgcggatt atctttctac gcaaggagta tttatctcta ctcccgtcaa
      181 tgattgcatc tcttttctct gctaacggtg tcgcggcggc cattgattta tgccagggat
      241 atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt acgcaggtct
      301 ggagtatcgc cgatgggcaa tggctggttt tttcggatac gaccaataat gccagcggtg
      361 gggccgtatt tttgcaacaa ggagcggaat ttacattatc accagaaaat gaaactggaa
      421 tgactctgtt tgccaataac accgtttcag gagaatataa taacggcggg gcaatatttg
      481 ctaaagaaaa ctcaacgctg aatcttacgg atgttatttt ttctggtaac gtcgcaggcg
      541 gctatggtgg cgcaatctat tcttctggta ctaacgatac cggtgccatc gagttacgtg
      601 tcactaacgc cgtgtttcgc aataacatcg ctaatgacgg caaaggtggt gcaatttata
      661 ccatcaataa tgatatctat ttaagtgatg atgtttttaa caataaccag gcatatacat
      721 caacaagtta cagtgatggc gatggcggcg caatcgatgt cacagataat aatagcgaca
      781 gcaagcatcc ttcaggttat acgataataa ataacactgc ctttacaaat aacactgccg
      841 aaggttatgg cggggcgata tataccaata gcgcgacggc tccctatctt attgatattt
      901 ctgttgatga cagctacagc cagaacggag gcgtgttagt cgatgagaac aatagcgcag
      961 caggctatgg agatggtcct tcctctgcgg cgggtggctt tatgtatctc ggcttaagtg
     1021 aagttacatt tgatattgcc gacggaaaaa cgctggttat tggcaataca gagaatgacg
     1081 gagctgttga ctctattgct ggtaccgggt taatcaccaa aacaggttcc ggcgatctgg
     1141 tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac ggtgaagtta
     1201 ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag gacgatccgc
     1261 aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag gcagagctaa
     1321 atgttggctc cacccaacaa acctttgagc actcattgac gggctttcag aatggcactt
     1381 taaatatcga tgctggtggc aatgttactg ttaatcaagg cagttttgct ggcaccatcg
     1441 aaggtgctgg tcagctcacc attgcgcaaa acggcagcta tgtgctggcg ggggcgcagt
     1501 cgatggcgct aaccggcgat atagtggtgg atgctggtgc ggtgctttcg ctggaaggcg
     1561 acgcggcaga tcttgccgct ctccaggacg atccgcagtc gatcgtgtta aacggcggtg
     1621 tgctcgatct ctctgatttc tccacctggc agagcggtac atcatacaaa gatggccttg
     1681 aagtcagtgg cagcagcgga acggttatcg gcagtcagga tgtggtagat cttgcaggcg
     1741 gaaacgatat gcatatcggc ggcgacggga aagatggcgt ctacgtggtg atcgatgcgg
     1801 gtgacgggca ggtcagcctg gcaaatgaca atcaatacct cggcacaacg caaatcgctt
     1861 ccggtacgct gatggtgagc gacaactcgc agcttggaga tacccattat aaccgccagg
     1921 ttatctttac cgataagcca caagaaagcg tgatggagat tactgccaat gtcgatactc
     1981 gctctacaac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt gaagtggcag
     2041 ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc gggcagcatc
     2101 aggatgaggg tagcacattg acgaaaacgg gggcgggtac gctggagctg accgccagcg
     2161 gtacaacgca gtcggcggta cgtgtcgaag aaggcaccct gaaaggtgat gttgctgata
     2221 tccttcctta tgcttcatca ctgtgggttg gtgacggggc aacgttcgag actggcgcgg
     2281 atcaggatat tcagtctatc gatgctactt ccagcggcac tatcgacatc agcgatggta
     2341 cggttttgcg cctgaccggg caggatactt ccgtcgccct tgatgcctca ctgtttaacg
     2401 gcgatggtac gctggtgaat gccaccgatg gcgtgacgtt gacaggtgag cttaatacca
     2461 accttgaaac tgacagcctg acttatcttt ccgacgtaac agttaatggc aatctgacca
     2521 atacgaccgg tgcggtcagc ctgcaaaatg gcgtcgctgg cgatacgctg acggtaaacg
     2581 gtgattacac cggcggcggt acgttactgt tcgacagcga attaaacggc gatgactcag
     2641 caagcgacca gttggtgttg aacggtaata ctgctggcaa cacaactgtg gtggttaact
     2701 ccattacagg gattggtgag ccgacatcga caggcattga agtggttgat ttcgcagctg
     2761 atcccacgca gtttcaaaac aatgcgcagt tcagtctggc aggcagcggc tacgtcaata
     2821 tgggagcgta tgactacacg ctggtggaag ataacaacga ctggtatctg cgatcgcaag
     2881 aagtaacgcc gccatcgcca cctgatccag acccgactcc cgatcctgat cccacgccgg
     2941 atcctgatcc aacacccgac ccggaaccta cgcctgctta ccagccggtg ttgaatgcca
     3001 aagttggcgg ttatctcaat aacctgcggg cggcaaatca ggcgtttatg atggagcgac
     3061 gcgatcacgc aggtggcgat ggtcagacgc tgaatttacg tgttatcggc ggagattatc
     3121 attacacagc ggcagggcaa ctggctcagc atgaagacac ttctacggtg cagcttagcg
     3181 gtgacctgtt tagggggcgc tggggcacgg atggcgagtg gatgcttggg attgttggtg
     3241 gctacagcga taaccagggc gacagccgct cgagtatgac cggaactcgc gccgataacc
     3301 agaaccacgg ttatgccgtt gggctaacct caagctggtt tcagcacggt aatcagaagc
     3361 agggggcctg gctggataac tggttgcagt acgcgtggtt tagcaatgat gtttctgaac
     3421 atgaagatgg cgtggatcat taccattcgt cggggattat cgcctcgctg gaagcggggt
     3481 atcagtggtt accggggcgt ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg
     3541 gcgtgcagca ggatgatttt accgccgcta accgtgcgcg cgtgtcacaa tcgcagggtg
     3601 atgatattca gacgcggctg ggtttacaca gcgaatggcg taccgctgtt catgtcatac
     3661 caacattaga tctgaattat tatcacgatc cccattcgac ggaaattgaa gaggatggca
     3721 gcactatcag tgacgatgcg gtgaagcaac ggggtgaaat aaaagtggga gtcacgggca
     3781 atatcagtca gcgagtgtcc ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt
     3841 ttgcccagac ggcagggttt ttgtcgatga cggtgaaatg gtaatcgctg tctgaccatc
     3901 actattgccg gatgcggcgt aaacgcctta tctggcctac ggagagcatt tgtaggcctg
     3961 ataagacgcg gcaagcgtcg catcaggcat tggcgcggtt gccggatgaa tccaaacaac
     4021 aacctcactt attctgcgtg tgcagcatat agttcacatc cacgccgggg ccgagtttaa
     4081 aagtattagt gatcgggttg taatgtagcc cggttatatg tcgctcttta aggcttgtct
     4141 gatctaccca gccaagcagc tctgccggtt taataaactt cttcacatca tgcgtgcctt
     4201 tgggcaccat gcgcaaaata tattccgcgc caaccaccgc catcagccat gacttgccgt
     4261 tgcggttaag cgtcgagaaa aagacatcgc cgcccggttt caccagttgc gcacaggctc
     4321 tgaccactga ctgcggatcg gggacgtgct ccagcatctc catgcaggtc accacgtcat
     4381 actgcccggc atgttttgcc gcgtgctctt ccacagtttc ttgcacgtaa tccacttgaa
     4441 tgccgctttc cagcgcgtgc agttttgcca cctgcaatgg ctcaaagccc atatccagac
     4501 cggtcaccgt cgcaccttcg cgcgccatac tctcggccag aatgccgccg ccacaaccga
     4561 catcgagcac ctttttgcca aataagccgc cagcacgctc ggcaatatag cccagacgca
     4621 gcgggttaat gcggtgcagc ggtttgaact caccttccag atcccaccag cgggaggcga
     4681 cggcttcaaa tttagcgatc tcttcgtggt ctacgttatg gtttaccggc gatttttcgg
     4741 cattcattgg cacttctact ccgtaattgg caagacaaac gagtatatca ggcattggat
     4801 gtgaataaag cgtataggtt tacctcaatc tgcgcggctg tgttataatt tgcgaccttt
     4861 gaatccggga tacagtagag ggatagcggt tagatgagcg accttgcgag agaaattaca
     4921 ccggtcaaca ttgaggaaga gctgaagagc tcctatctgg attatgcgat gtcggtcatt
     4981 gttggccgtg cgctgccaga tgtccgagat ggcctgaagc cggtacaccg tcgcgtactt
     5041 tacgccatga acgtactagg caatgactgg aacaaagcct ataaaaaatc tgcccgtgtc
     5101 gttggtgacg taatcggtaa ataccatccc catggtgact cggcggttta tgacacgatc
     5161 gtccgtatgg cgcagccatt ctcgctgcgt tacatgctgg tagacggtca gggtaacttc
     5221 ggttccatcg acggcgactc tgcggcggca atgcgttata cggaaatccg tctggcgaaa
     5281 attgcccatg aactgatggc cgatctcgaa aaagagacgg tcgatttcgt tgataactat
     5341 gacggcacgg aaaaaattcc cgacgtcatg ccaaccaaaa ttcctaacct gctggtgaac
     5401 ggttcttccg gtatcgccgt aggtatggca accaacatcc cgccgcacaa cctgacggaa
     5461 gtcatcaacg gttgtctggc gtatatcgat gatgaagaca tcagcattga agggctgatg
     5521 gaacacatcc cggggccgga cttcccgacg gcggcaatca ttaacggtcg tcgcggtatt
     5581 gaagaagctt accgtaccgg tcgcggcaag gtatatatcc gcgcccgtgc tgaagtggaa
     5641 gttgacgcca aaaccggacg tgaaaccatt atcgtccacg aaattccgta tcaggtaaac
     5701 aaagcgcgcc tgatcgagaa gattgcggaa ctggtaaaag aaaaacgcgt ggaaggcatc
     5761 agcgcgctgc gtgacgagtc tgacaaagac ggtatgcgca tcgtgattga agtgaaacgc
     5821 gacgcggtcg gtgaagttgt gcttaacaac ctctactccc agacccagtt gcaggtttct
     5881 ttcggtataa acatggtggc attgcaccat ggtcagccga agatcatgaa cctgaaagac
     5941 atcatcgcgg cgtttgttcg tcaccgccgt gaagtggtga cccgtcgtac tattttcgaa
     6001 ctgcgtaaag ctcgcgatcg tgctcatatc cttgaagcat tagccgtggc gctggcgaac
     6061 atcgacccga tcatcgaact gatccgtcat gcgccgacgc ctgcagaagc gaaaactgcg
     6121 ctggttgcta atccgtggca gctgggcaac gttgccgcga tgctcgaacg tgctggcgac
     6181 gatgctgcgc gtccggaatg gctggagcca gagttcggcg tgcgtgatgg tctgtactac
     6241 ctgaccgaac agcaagctca ggcgattctg gatctgcgtt tgcagaaact gaccggcctt
     6301 gagcacgaaa aactgctcga cgaatacaaa gagctgctgg atcagatcgc ggaactgttg
     6361 cgtattcttg gtagcgccga tcgtctgatg gaagtgatcc gtgaagagct ggagctggtt
     6421 cgtgaacagt tcggtgacaa acgtcgtact gaaatcaccg ccaacagcgc agacatcaac
     6481 ctggaagatc tgatcaccca ggaagatgtg gtcgtgacgc tctctcacca gggctacgtt
     6541 aaatatcagc cgctttctga atacgaagcg cagcgtcgtg gcgggaaagg taaatctgcc
     6601 gcacgtatta aagaagaaga ctttatcgac cgactgctgg tggcgaacac tcacgatcat
     6661 atcctgtgct tctccagccg tggtcgcgtc tattcgatga aggtttatca gttgccggaa
     6721 gccactcgtg gcgcgcgcgg tcgtccgatc gtcaacctgc tgccgctgga gcaggacgaa
     6781 cgtatcaccg cgatcctgcc ggtgaccgag tttgaagaag gcgtgaaagt cttcatggcg
     6841 accgctaacg gtactgtgaa gaaaaccgtc ctcaccgaat tcaaccgtct gcgtaccgcc
     6901 ggtaaagtgg cgatcaaact ggttgacggc gatgagctga tcggcgttga tctgaccagt
     6961 ggcgaagatg aagtaatgct gttctccgcc gaaggtaaag tggtgcgctt taaagagtct
     7021 tctgtccgtg cgatgggctg caacaccacc ggtgttcgcg gtattcgttt aggcgaaggt
     7081 gataaagtcg tctctctgat cgtgcctcgt ggcgatggcg caatcctcac cgcaacgcag
     7141 aacggttatg gtaaacgtac cgcagtggcg gaatacccga ctaagtcgcg tgcgacgaaa
     7201 ggggttatct ccatcaaggt taccgaacgt aacggtttag ttgttggcgc ggtgcaggta
     7261 gatgactgcg accagatcat gatgatcacc gatgccggta cgctggtacg tactcgcgtt
     7321 tcggaaatca gcatcgtggg ccgtaacacc cagggcgtaa tcctcatccg tactgcggaa
     7381 gatgaaaacg tggtgggtct gcaacgcgtg gctgaaccgg ttgacgagga agatctggac
     7441 accatcgatg gcagtgctgc ggaaggagac gacgaaatcg ctccggaagt ggacgttgac
     7501 gacgagccag aagaagaata attttacttc ttcatgccaa aagggagcta tctcccttgt
     7561 ttgaattgaa aagtccaggc tgcaaagtct gggcttttgt cgtattaggg cacggtaaag
     7621 tttggctgtg cccgtaaaaa atggctggct atacacaagg aatgtggcaa tgagtggtga
     7681 aaaaaaggcg aaaggctggc ggttctatgg tcttgtaggt tttggcgcaa tagtactgct
     7741 ttccgctggc gtctgggcgt tgcaatatgc tggcagtggg ccagaaaaaa cgttgtcgcc
     7801 gctggtggtg cacaacaatc tgcaaatcga tctcaatgag ccggacctct ttctcgacag
     7861 cgactctctg agccagcttc ccaaagatct cctcaccatt ccgtttctcc acgatgttct
     7921 gagtgaagat ttcgttttct attatcagaa tcatgccgat cgtctgggca ttgaaggcag
     7981 cattcgtcgc attgtctatg aacacgatct cacgctgaaa gataaactct tttcgtcact
     8041 cttagatcag cccgcacagg cggcgctatg gcacgataaa caaggccatc tttcacatta
     8101 tatggtgctt atccagcgta gtggcttaag caagctgctg gagccattgc tgtttgccgc
     8161 taccagcgac agccagttaa gtaaaacgga aatcagtagc atcaagctaa ataatgaaac
     8221 tattcctgtt tatcagttgc gctataacgg caataacgcc ctgatgttcg cgacatatca
     8281 ggacaagatg ctggtgtttt ccagcacgga tatgctgttt aaagatgaag agcaggatac
     8341 cgaagctacg gcgatcgcaa gtgatttgtt gagcggtaaa aaacgctggc aagtcagctt
     8401 tggcctggaa gagcgtactg ccggaaaaac accagtacgc cagcgcatcg tggtcagcgc
     8461 caggttgctg ggttttggct accagcgatt aatgccttct tttgccggag tgcgtttcga
     8521 aatgagcaac gacggctggc atagctttct ggcgttaaat gatgaatccg ccagcgtaga
     8581 taacagtttc gattttacgc cggtctggaa cagtatgcct gccggggcca gcttctgtgt
     8641 ggcggtgccg tattcacacg gtattgccga agagatgctt tcgcacatca gccaggaaaa
     8701 cgacaagctg aatggggcgt tagacggtgc cgcggggctg tgctggtatg aagactcaaa
     8761 attgcaaacc ccgctgtttg tcggtcagtt tgatggcact gccgaacagg cgcaattgcc
     8821 agggaaactg tttacgcaaa atattggtgc gcacgaaagc aaagcgccag aaggtgtttt
     8881 gccggtaagc cagactcagc agggcgaagc gcaaatctgg cgtcgcgaag tgagttcccg
     8941 atacggtcag tatccgaaag cgcaggcggc gcaacccgat caattaatgt cggattattt
     9001 tttccgcgtg tcgctggcga tgcaaaacaa aacgctgctt ttctccctcg atgacacgct
     9061 ggttaataac gccctgcaaa cactgaataa aacccgcccg gcaatggtgg atgtaatacc
     9121 cactgatggc atcgttccgc tctatatcaa tccacaaggc gtggcgaaac tgctgcgtaa
     9181 cgaaacgttg accagtctgc cgaagaatct cgaaccggtt ttttataacg ccgcacaaac
     9241 tttattaatg ccgaagctgg acgctttatc tcaacaaccg cgttatgtca tgaagctggc
     9301 ccagatggaa cccggtgccg cctggcagtg gctacccata acctggcagc cgctatgagg
     9361 cacgggctgc tggcgctgat ttgctggctg tgttgtgttg ttgcccatag cgaaatgctg
     9421 aatgtcgaac aatccgggct gtttcgcgcc tggtttgtac gcattgcaca agaacagctc
     9481 cgctaggggc caagtccacg ctggtatcag caggattgtg cgggcctggt gcgatttgcg
     9541 gcgaacgaga cgctgaaagt tcacgacagt aaatggctaa aaagtaacgg tttatctagc
     9601 cagtatttgc cgccagagat gacgctaaca cctgaacagc gtcaactggc gcaaaactgg
     9661 aatcagggga acgggaaaac cggcccctac gtgaccgcga ttaatttgat tcagtacaac
     9721 agccagttta ttggccagga cataaaccag gcgctgcctg gcgatatgat ttttttcgat
     9781 cagggcaatg cccagcactt aatggtctgg atggggcgtt acgtcatcta ccacaccgga
     9841 agcgccacga aaactgacaa cggaatgcgc gcagtcagtc tgcaacaact tatgacatgg
     9901 aaggacaccc gatggatacc caacgattcc aatcccaatt tcattggcat ttatcgttta
     9961 aattttctgg cgcgatagca gcgtgcttgt ccctgtctct tgtgggaaca gggcttgcta
    10021 atgctgatga ttcgcttcct tccagtaact atgcgccgcc cgcaggggga acattctttt
    10081 tgcttgctga cagcagtttt agcagcagtg aagaggcgaa agtgcgactg gaagcgccgg
    10141 ggcgtgatta tcggcgctat cagatggaag agtacggcgg cgtggacgtt cgcctgtatc
    10201 gtattcctga cccgatggca tttttgcgcc agcagaaaaa cctgcatcgc attgtggtgc
    10261 aaccgcaata tttgggcgac gggctgaaca atacgctgac ctggctgtgg gataactggt
    10321 acggcaaatc tcgccgcgtg atgcagcgta ctttctcttc tcagtcacgg cagaatgtga
    10381 ctcaggcatt acccgaatta catctcggca atgccattat taaaccttcc cgttatgtac
    10441 agaacaacca gttttccccg ctgaaaaaat atcccttggt ggaacagttc cgttatccac
    10501 tatggcaggc taaaccggtc gagccgcagc aaggggtaaa actggacggc gcatccagca
    10561 atttcatctc gccgcagccg ggtaacattt atattcctct cggccaacaa gagccgggac
    10621 tgtacctcgt cgaggcgatg gttggtgggt atcgggcgac gacggtggtg tttgtttccg
    10681 ataccgtggc gcttagcaaa gtgtcaggca aagagcttct ggtgtggacc gcgggtaaaa
    10741 aacagggtga agcgaagccc ggctcagaga tcttgtggac tgacggtctt ggcgtgatga
    10801 cccgcggtgt gaccgatgac agcggtacct tgcagttaca acatatatcg ccagaacgtt
    10861 catacattct gggtaaggat gctgaaggcg gcgtttttgt ctccgagaac ttcttctacg
    10921 aaagcgaaat ctacaacacc cgcttgtata tttttaccga tcgcccgcta tatcgcgcag
    10981 gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca cgatccgttg cattcatccc
    11041 ccatcgtcag cgccccggcg aagctttcgg tgctggacgc taacggcagt ctgttgcaaa
    11101 ccgtcgatgt cacgctggat gcgcgcaatg gcgggcaggg aagtttccgc ctgccagaaa
    11161 atgccgttgc cggaggttat gagttacgtc ttgcttaccg caatcaggtc tatagcagta
    11221 gttttcgcgt agccaactat atcaagccac atttcgagat tggtttagct ctcgacaaaa
    11281 aagagttcaa aactggcgaa gcggtcagcg gcaaactgca acttctctac ccggatggcg
    11341 agccggtaaa aaatgcccgc gtgcagttaa gtttgcgcgc tcagcaatta tcaatggtcg
    11401 gtaacgattt gcgttatgcc ggacgtttcc ccgtgtcgct ggaaggcagt gaaacggtgt
    11461 ccgacgccag cggtcatgtg acgttaaatc tccccgccgc cgataaaccg agccgctatt
    11521 tgttaaccgt ctccgccagt gacggcgcgg cgtatcgcgt caccaccacc aaagagatcc
    11581 tcattgaacg cggcctggcg cattactctt taagtaccgc cgcacaatac agtaatagcg
    11641 gcgagtcggt tgtgttccgt tatgccgcgc tggaatcttc aaaacaggtt cctgttacgt
    11701 atgaatggtt gcgtctcgaa gaccgcacga gccatagcgg agagctaccg tctggcggca
    11761 aatcctttac cgtcaatttc gataaacctg gcaactacaa tctgacgtta cgcgataaag
    11821 acggcttaat tctcgctggg ttaagtcatg ccgtcagcgg taagggcagc atgtcgcata
    11881 ctggtacggt agatatcgtg gcagataaaa cgctgtacca gccaggcgaa accgcgaaga
    11941 tgttgattac ctttccagaa ccaattgatg aagccttgct gacgctggaa cgcgatcgcg
    12001 tggaacagca gtcgctgctt tcgcacccgg caaactggat aacgctacaa cgcttaaacg
    12061 atacccagta tgaagcccgc gttccagtga acagtacctt tgcgcccaac atcacttttt
    12121 cggtgctgta cacccgtaac gggcagtaca gttttcagaa cgcagggatc aaagtcgccg
    12181 ttccccagtt agatattcgg gtgaaaacgg ataaaaccca ctaccagccg ggcgaactgg
    12241 tcaacgtcga attaacctcg ttgctgaaag gtaaaccggt ttctgcgcag ctaacggttg
    12301 gtgtggtcga tgaaatgatc tacgcgctac aaccggaaat cgcgccgaat atcggcaaat
    12361 ttttctatcc cctggggcga aacaatgtgc gcaccagttc cagcctgtca tttatcagct
    12421 atgaccaggc gctctccagc gagcctgttg cgcctggcgc gactaaccgg agcgagcggc
    12481 gagtaaaaat gctggaacgt ccacggcgtg aagaggtgga taccgctgca tggatgccgt
    12541 cgctaacaac ggataaacaa ggcaaagcgt actttacgtt cctgatgccg gattcgttaa
    12601 cgcgctggcg cattaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg
    12661 cttatctgcg ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg
    12721 tgggcgacaa accgtcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg
    12781 cgctggtgac taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg
    12841 cgaattatat ttcgctggcg cagaacattc agcaatctgg cttgttaagc gcagaactgc
    12901 aacaaaatgg gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gataacagct
    12961 ggcccgttga acagcagaaa aatgtcatgc tcggtggtgg cgataacgcg ctgatgttgc
    13021 ccgagcaggc gagcaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca
    13081 acaatcttga tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc
    13141 tgatcccgct cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg
    13201 acattcgtca gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag
    13261 cgcgctttac ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat
    13321 ggtacgccga ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc
    13381 agcatatgct cgacagttat gccgagcagg cagataacat gccgttattg catcgggcgc
    13441 tggtgctggc gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaagggttgg
    13501 atgaagctat cgcccggcgc ggaactaaaa ctgaagattt ctctgaggaa gccccccgcg
    13561 atatcaatga cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa
    13621 acgtcttaac catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg
    13681 ttcagcaata tgcgtgggat aaagcggcaa acagcaatca gccgctgacg cacacggttg
    13741 tgctgctcaa tagcgggggc gacgctaccc aggcggctgc tattttaagt ggtttgaccg
    13801 ctgagcaatc tactattgag cgcgcgctgg ccatgaactg gctggcgaaa tatatggcga
    13861 caatgcctcc agttgttttg cctgcgcctg cgggcgcatg ggccaaacat aagttaactg
    13921 gagggggcga atactggcgt tgggttggtc agggcgtgcc ggacattctc tcttttggtg
    13981 acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga accggcaaaa acggctcaac
    14041 aaagtaacat tccggtgacc gttgaacgcc agttgtatcg gcttatcccc ggtgaagaag
    14101 agatgagctt tactctgcag ccggtgacca gcaatgagat tgacagcgat gcgctgtatc
    14161 tcgatgaaat tacgcttacc agcgagcagg atgcagttct gcgctatggt caggtggaag
    14221 taccgctccc gccgggagcc gacgttgagc gcacaacatg gggcatttca gtcaataaac
    14281 ccaacgccgc gaaacagcag gggcaattgc tggaaaaagc gcgtaatgaa atgggcgaac
    14341 tggcctatat ggtgccggtg aaagaactga cgggaacggt cactttccgc catttgctgc
    14401 gcttctcgca aaaagggcaa ttcgttctgc ctcctgctcg ttatgtgcgt tcctatgcac
    14461 ctgcacagca aagtgttgcg gcagggagtg aatggaccgg gatgcaggtg aaataagtga
    14521 actggcgcag aatcgtctgg ctattggcgt tggttactca gccaacgctt gcggaggaga
    14581 cgccgttaca actggcgctg cgcggtgcgc agcacgatca actgtatcag ctgtcgtcat
    14641 caggcgtgac caaagtctca gcattacctg actcgctcac cacgccgtta gggagtttgt
    14701 ggaaactgta tgtttacgcc tggctggagg atacccatca accggagcag ccctaccagt
    14761 gtcgcggaaa ctccccggaa gaggtttatt gttgtcaggc tggggagagc ataacgcgcg
    14821 ataccgcgct ggtacgttcc tgtgggctct attttgcgcc gcagcggttg catattggcg
    14881 cagatgtgtg ggggcaatac tggcaacagc gtcaggcacc tgcgtggtta gcgtctctta
    14941 ctacgctgaa gccagaaacc tcggtaacgg ttaaatcgct gcttgattca ttagcgacgt
    15001 tacctgcgca aaacaaagct caggaagtct tactggatgt ggtgctggac gaggcgaaaa
    15061 tcggtgtcgc atccatgctc ggtagtcggg tacgggtgaa aacctggagc tggtttgccg
    15121 atgataaaca ggaaatccgt cagggtggtt ttgctggctg gttaaccgat ggtacaccgc
    15181 tgtgggttac tggcagtggc accagcaaaa cggtactgac ccgttatgcg acggtgctta
    15241 atcgggtttt gccagtgccg acacaagttg ccagtgggca atgtgtagag gtggagctat
    15301 ttgcgcgcta tccgctgaag aaaatcacag cggaaaagag tacgacggca gtaaaacccg
    15361 gcgtacttaa tgggcgctat cgtgtgacct ttaccaatgg taaccacatt acgtttgtca
    15421 gccacggtga gacgacttta ctgaacgaaa agggcaaact gaaattgcag tcgcatctcg
    15481 atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc gaaaagtacg ccacctgagg
    15541 cagcgaaagc catgaccgtg gcgatacgca cttttttaca gcaaaacgcc aatcgggaag
    15601 gcgattgcct gaccatcccc gacagcagcg ccacgcagcg tgtttctgct tcgccagcta
    15661 ccacgggggc gcgaacgatg accgcctgga cgcaggatct catctatgcg ggcgatccgg
    15721 ttcattatca cggcagtcgc gctaccgaag ggacgctttc ctggcggcaa gccatggccc
    15781 aggcggggca gggagatcgt tacgaccaga tactcgcgtt tgcttatccc gacaacagcc
    15841 ttagccgctg gggagcgcca cgctcaacct gtcagttatt acccaaagcg aaagcctggc
    15901 tggcgaaaaa aatgccgcag tggcggcgta tattacaagg cgagacgggg tacaacgaac
    15961 cagacgtgtt tgcggtctgt cgtttagtct caggtttccc ctataccgat cggcagcaga
    16021 aacggctgtt tattcgtaac ttctttacgc ttcaggatcg gctggattta acccatgagt
    16081 atctgcacct ggcctttgat ggttatccca ccgggcttga tgagaactat atcgaaacgc
    16141 tgacccgaca attattaatg gactaatgct atgcgaaaga tttttcttcc gttgttactg
    16201 gtggcgcttt cgcctgtcgc tcacagtgaa ggtgtgcagg aagtcgagat tgacgcaccg
    16261 ctctcaggct ggcatccggc agagggcgaa gatgccagtt tttcacaatc gatcaattac
    16321 ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct cagcgcagat ccacggcaaa
    16381 ataaagaatt atgctgcggc gggtaaagtt cagcagggcc ggctggtggt caacggtgcc
    16441 agtatgccgc agagaattga atccgatggt tcatttgcac gcccttatat tttcactgaa
    16501 ggcagcaaca gcgtgcaggt catcagcccg gatgggcaaa gccgacaaaa aatgcagttt
    16561 tactcaacgc cgggtacagg aacgattcgt gcacgtttac ggctggttct ctcgtgggat
    16621 acggacaata ccgatctcga tctccatgtt gtcacgcccg atggcgaaca cgcctggtac
    16681 ggtaacacgg tgctgaaaaa cagtggtgca ctggatatgg atgtcacgac ggggtacgga
    16741 cccgagattt tcgccatgcc agcgccagtt cacggacgct atcaggtgta tatcaactac
    16801 tatggtgggc gcagcgaaac ggaattaacc accgcccaac tgacgctgat caccgatgaa
    16861 ggatcggtca atgagaaaca ggaaacattt attgtaccga tgcgtaatgc tggcgagctg
    16921 acgctggtga aaagttttga ctggtgatcc atcccttcac acaacctggc tctttctttt
    16981 ttcagataag actgctgggc gatttgccga cgccaggata taaccattaa caatcgtcgg
    17041 cagattggtc atttcttatt tttcgtactg agaactgtcc atttactccg gctttcctgg
    17101 ttaaggcaat ggtttctaag cgatacgcat tattgcaatg gtgacagaac attgcggtaa
    17161 cacgctttta ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc
    17221 tttgaaatac cttgcttctt ttcgtacaac tctgaaagcc tcgcgctaca tgttcagagc
    17281 attggcgtta gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc
    17341 gttacatcag cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca
    17401 gcgctttatt caacgcacct ctgatgtgat gaaagagctg aagtacatcg ctgaaaatcg
    17461 cttatcggca gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc
    17521 tgcgtttgaa ccgctgtttg ccgactctga ttgttccgca atgagtaaca cctggcgagg
    17581 ttctctggag tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta
    17641 cgatctcaac cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct
    17701 gcgtgatatg ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa
    17761 atatcgaaat gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc
    17821 gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt tatctggcga acaggttgca
    17881 ggcgcttttg ggtgtcgagc agaccatccg gatggagaat tttttcttac cgggtacgtt
    17941 gccgatgggg gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc
    18001 agaaagtaaa attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac
    18061 ggaagggttc cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt
    18121 gtattcggtg ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat
    18181 tttgctgaat gtgcttgccg gagctgcatt gtttactctc gcgcggatgt acgagcgacg
    18241 tattttcatt ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg
    18301 caagattgtc gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa
    18361 tattttaagt aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca
    18421 acgactgaca caaattatct gtgggcagca agtcaatttt gttgatgtcc tgaccagcaa
    18481 caataccaat ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat
    18541 ttgtgtgctg gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc
    18601 acaagcagcg gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga
    18661 gctgcgaacg ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt
    18721 accgaaaggc gtcgatcgtc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa
    18781 aattatcagc gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc
    18841 gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt
    18901 ggtacgcaag cagttaggct tgtactgctt tattgaaccg gatgtgccag tggctttaaa
    18961 tggcgacccg atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt
    19021 caccgatacc ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg
    19081 tgttcgcgat accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt
    19141 cttccaggtc ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat
    19201 ttgtgaaaaa ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat
    19261 gggcagccag tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg
    19321 cgtggaaggg ctgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca
    19381 gttcctggaa accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga
    19441 accgactccc gaagatgtgt taatcactga cgaggtagtg agtaaaaaat ggcagggcag
    19501 agcggtagtg accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga
    19561 gtgggtacac agtgtggctg caccgcatga gctaccggca ttgttggcgc gtatttattt
    19621 gatcgagatg gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag
    19681 cgataatgac gatatgatga ttctggtcgt ggatgatcat ccgattaacc ggcgtttgct
    19741 ggcagatcag ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gcgtcgatgc
    19801 gcttaatgta cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgcccaa
    19861 tatggatggt taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat
    19921 cggagtaaca gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga
    19981 cagctgtctg tcgaagccag taacgctgga tgtgataaaa cagacgctga cggtatatgc
    20041 cgagagggtc aggaaatcgc gggaatcgta ggccggataa ggcgtttacg ccgcatccgg
    20101 cattcagtgc aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttaatgtaa
    20161 tctgttgaaa taatgggaat cgtaggccgg ataaggcgtt tacgccgcat ccggcattca
    20221 gtgcaaatgc cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta
    20281 tctgccggac ttaaggtcac agaagagaga taattcagca gggcgatatc gttctcgaca
    20341 cccagcttca tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc
    20401 tttttagcga tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct
    20461 tttggcgaga gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa
    20521 acgctttccg gggtaaattt cttccctttc tgcagcgcgg cgagggcttt cggcagatcg
    20581 gtcggtgcac cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga
    20641 atcgccgggt tgttgttcat ggtcagaaca ataatcgaca ggcttgggaa atggcgcttg
    20701 atgtacttaa ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta
    20761 atcaacacat gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat
    20821 tcgccgacaa cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact
    20881 atcggatggt catcggcaat aattacgttc atattgttca tgtacttgta cctatgtatt
    20941 aactatttga ttttaaaaat atattatatg ctttgtttag tcgttggggc acctgtggga
    21001 cataagtaca tagctttctg tttagtactt ctacctggga ctggttattg tctttcatcc
    21061 atgcaccgta aacattgtaa accatctggg cattcgcatg tcccatctgg ttggctatga
    21121 agtttggatt tgctccggca gttaaagccc agcaagcata agtatgccga gactgatacg
    21181 cttttctatg gcgaattttt gctcgtttta atgcagattc ccatgttctg ttgatggatg
    21241 ttacggcata atgaactcct gagttacctg tgcgatctaa tatctgagga ttgaaaacaa
    21301 agctgcatga gtgagtgctt gtccggccat actctcgcag ctttacttct atctgatatt
    21361 gtttaccaag cctggttaat gtcgcctgat tttttaatac gtcaattgct ggctgtatga
    21421 gttgtattac cctgtttgtt cctgcatctg ttttgggtag tgtaaattcc tttgtctgag
    21481 tgtaattcct tttgacggtc aaggtacccg cttccagatc gatatcctcc catgccagcc
    21541 cacacagttc gccatgccgc atcccggtat atacagccag cgaccagaaa tttttagttt
    21601 gctggtggtg gcacgcatca atcagtctgg caaactcatc ttttgttagt ggatcgggag
    21661 gtgttttcgc tcttttcagt gatggaagct cactgaaagg gtttttgcct atatacccat
    21721 tgtttgccgc aaaacggaac atcccggaga taattgtcat atagctattt acagtaggta
    21781 cactacgacc tttaataggc ttcctctgat tttttcctgg caactgatat cccgttaata
    21841 attctttacg aaaaatgaga acatcttcct gtgtaattga tgatgccagt ttgttccctc
    21901 caaggcagtc aaggcaattt cggatagctg atttgtatct gaccatggca tttgctgtta
    21961 tttccatttt cttaagctca agccattttt ccgaaagtgc cttaatggtt atctcttttt
    22021 ttcccagacc aaagtgtttc aggttagggg aattagggaa ctgcgcggcg tagtcgaagc
    22081 tccccattct gattgcaaaa caaacggaag tacgaagttc acctgcgatc ttccggtttt
    22141 tggcggtgtc aggaacaccg aggttttctc tgacacgttt gccgttatag tgaaaccata
    22201 tgcggagtga tccgccatgg ttttcaacgc ctgtcgggta tgatgcgtta ctcattaaac
    22261 ctcccagacg tccaggagca ttaacaggtt aaccggaact tgcatttttg gcacctggtt
    22321 gtttctggtt ttcgatccag cgcataattt cctcaatgtt gtatacacat tcactgaaag
    22381 gcccgggctc tccttctgct gcgtaatgac ggtattcttt ccccagtaac caggattttt
    22441 ttcttgctct ggcaatggtt cctgatttca gtccagttgc agcaataagg gttctttctg
    22501 tgcaccattt gctgggcgtt atctgataga tgattgtttc cattcctgac tcacactatc
    22561 ttcaggccac ggcagtggca ccacacgtca aacattcgct ttacaacttc acggcagtag
    22621 aagccgtgag catctctggt taaatcgtat cggtacccgt atctgagtcg tatccatatt
    22681 tcaaactcgc gattcacgac ttattctcct gctgtcttta tgccagttca tccggttgcg
    22741 ctggggccca ggccttccgt ggcctgtatt tgcggcgttt tctggcttca atgcgtatct
    22801 ttgtcaccct gactggcttt ggttcgtgcc ggaacacttt gtttctgata ttacgccgtg
    22861 tattcatcat ccagataaaa cgctgggtat ggtcgcatcc gtcatcaacg gtaattactg
    22921 tgcggaccag ttcatcgtta atatcaacca tttcacccat tcactgagcc ctcatacgcg
    22981 aatttcgtgg cttccatcag ttgcgtccat acccattcaa gaaattgtgc gttttctcct
    23041 gagcggtcat attcatcgac gctgcggggc ttgatggtcg ccagtagtgg cacaatccgg
    23101 tacttcatct gttcggttaa cttgtccggt acaagtcgcc agccgttata tttcgatgga
    23161 acatggtggt attcagccag gaagcgtcgt atatcgtttt tcatttcctc gcatgagttc
    23221 tgatcatgaa gtgcagcgga aagccagcgt ccgatatatt cagggattcg cacatcaagt
    23281 actttttctg ccgtcgtcag cattgcctgt cggcaggcgt tccagccgtt gacatagtga
    23341 cgtgttgcat cccatgaaac ctgcacatca gggtaagcat cctgttctgt tctcactggc
    23401 ggtatctgag gaacagtgaa tagtggactg acgatatatt tatccagaga gcctccccgc
    23461 tcttctgcaa gactttccca cgatgtcatt gtggggaccc tgtaacctgc ttttttgtgc
    23521 gtatacatcc atgccactct gccgttatca tctgcggcgg caatgcgctg ccaggcagga
    23581 taaagcgggg tgtatgtttc tctgtcatta cgggcgtcag gcatggcatc aatactgcaa
    23641 acttcccccg taaggttatt cagccaggcc ttcgggctaa tctctgacgt tacgatttca
    23701 tccagttttt tctccagatt ttctatgtga ctgattagcg caaggataac tggcggagtg
    23761 gcaagggagt gaaatgcttc cagatcacat cccttgtctc catatacggt attttgtgcg
    23821 gcgtcacgca gtgctttcag atcagcttct ttcatcgttc agactccctt gttgctgttt
    23881 tgtaggcgcg caggatgtcg cgtgtttttc ctgataatga cgacttaacc aggaaaaagc
    23941 cgctccggct ttctgtgatg tccggcgtgc acagaagtac cgcatcaacg acacgattat
    24001 gttttcgcaa tgtcaggcgg gaactggtga taatcagttt tgcctgttcg ccgtaatcgg
    24061 tgaatgaaat aatcatctga tggcgatctc cgttatgaaa gtgttcctgc tggttcaatg
    24121 ctgataattt ctgctgcaat aagcatacct gccagccatg cttcgccgag catgtcctca
    24181 tccgtacatg caagatcacc gatctgtatt gtggccataa tgttagtttc accggtatat
    24241 tcatcctgtg cttcttcata cggcaggttt tcataaaggt tttcaattgc gcaggttatt
    24301 acatccagtc ccgtcagatt gccgacaacg tccacttcaa atgtttcgcg gtactcccat
    24361 tgcccgagtg tcagacgaac cgtctgtctt gccatgcgcc cgcataatgt catgttcggg
    24421 tcgtagttca tgattgccgc agtagtgcta ttcatgttct gcctccggtg tgtaaatagc
    24481 tttgtcgtgt ttaaattcac cgttccagtt tttcttcata gggagttcac cgttcatgta
    24541 aaactggtat aaacggtggc atcctttttc cagcaatacg ggggtgaatt tcgtgaatgt
    24601 tgctttcccg tgtggagtga tctgtactgc ttcttcggtc aggtatctgt cacgggcata
    24661 agaggccgtg cgccagcggg gtgttttctc cggatcgcgt tgttcgttga atacccattt
    24721 tcggccagac agccaccaca ttatctggct ggtatttacg ccgttcagcg cacggcagaa
    24781 ctggggaaca gtcatgcctt tagagaaatg tttttccaga ctgtctacgg ttgcagagag
    24841 cgttttattt tccagggcca tagcttcagc ccgttcttcg gcctcaataa ccatcaacgc
    24901 gagttccttg cgagtgagta cgcgctgaac atcgatgttt tcccgctgag tgaagtagaa
    24961 ctccacaagg tcttcgtgat agttccacgc ctgatccgtt tccagcaatt ttgcgtggtt
    25021 ggctgcgccg cgttcggtcc agaggatgag ggatcgaaca ttgctggcaa ttttcacaga
    25081 gtagctttga gatactctgt gcttaagttc acgtagttca tcgccttcca gcaaaaaata
    25141 atgctttcca acaacgaatc gttcttcgtt tcgggaatga ttctggcgaa tacggattgg
    25201 tgttgctcca taacctgccg ccagttgttc agtggtcaca acgcgttgac cgcgatattc
    25261 gataatttgc aaatcacggg ctgccaccgg cacaagttca gttgattttt tcatcattac
    25321 acctcacgaa aagctgatta gtggaacact ctggtttccg gttctgagtt gtcagtggct
    25381 tcactgataa gcccatccag aatcatgtgc atcgctctgt aaccttccat tgtgatgccg
    25441 gttttttcgc cgtcgcccag agcgccggtg aatgctttgt acataagaat gcttttgagc
    25501 agccctgatt ccgctccgta agtttctatg agtgcccatt ccatatagtt ctgcatagcg
    25561 agacgtaatg gtttcaggta gattgtcatg ccaccgttgt ggcctctgta catggtgccc
    25621 ttgtctgtac cgccattctc gttaagcact tcggtccagc cgtttctggt gagctgttca
    25681 gagaagaaca tggcggtata aagccagcgc cagtacatga ttgtcttttc cggcgctagc
    25741 tcacctgtca tgcctttttc ttctgcttca ctgatgcaac acactatttc cagaccatgc
    25801 cagatcggct cgtcaaaaca gccttcatcg agcatttcca gtgctttgtt gatatcaagc
    25861 agtccgtaag gtgtgtggat accttcagcg gtaagaaaaa ataactgttc agttgcagtc
    25921 atggtttgct ctcttcttaa atggtctttg cgcgacaagc cggcaatact gatcaatcca
    25981 ggcgttgtat ttttccatcc aggcatcaaa ctgttttctg cgtgcaagga tgcgcaacag
    26041 gcgatgaaaa caacgcccgt gagctctgta gtattcgggc gtaatttctc ccggatgaaa
    26101 gacttctcca tattccggat gcatcaggcg ctggtcaggc agacgttttt caaaacctga
    26161 actttcgaat accttcgttg tcataaaatg agcgagatgt atgatggccg tttttcgcgt
    26221 gaagcatttc ttaacttgtc cgtggcggca gactctgtaa acagggccgc aatcgagttc
    26281 gtatttatga aaagcacggt cgattccggc attatttatt gcactgtatt ccgcatcact
    26341 gaagaaaggt ggaaatacga tgtatccttc cattgtcagc gccagaacgc gcggatcggt
    26401 ttctgtcagc cattcgtttt ctgtgaattc ttcagaatta atttcaatga tttcaggttc
    26461 ttctttcatg gttactccgt gttcatccgg tgttaattac ttttattcac tctgagcgca
    26521 gccagaagat gtggctcagc aattcgtttg ttgcgatata tctgtgatac agcctggcct
    26581 tttcaggtcg taaaaatccc cgacaataaa gctgtaaatt aattactgtt cttaaataat
    26641 gttattcgtt ttcagtccgg cacagatgct tttctgcttt gtctgctgta caggataatg
    26701 agcggaaaag acaattcatc atgtttagcg catctggtga accaatatca ccgctgtcaa
    26761 taatcacctg taacaaagac aggctttctg ttatctcgca gtggacatta tcgagtactt
    26821 caacgcaatt attcattttt cattatctcc gttattccgg gggaggagct atgctgttaa
    26881 tgattaacgc atccggtaat atgcccctct ttaatgtaat cgatgcattc attcaaaagt
    26941 tcatcaatga ttaactttcc tgattcagtc agatactcac catgttggtt gataccgaca
    27001 gcatcctggt atgcagtgcg tattgctgtt tcaccatctt tccgcccaaa ctcaccacgg
    27061 gtaacgcctt caaatcgtaa tagcaactgg cttatgaact gttctgttat ttcaatagtt
    27121 gttaactctc catcatgacg ctttacagtg aggagattac ttcctgtttt tcgtttcatc
    27181 cgccgcagtg ctgcaaccgt tatgcggcga cgatatgcag aaatgtattc atctttcatt
    27241 tgttatttcc cgtatgcctt tttgagaaac aatactgcaa ctgaccagta tccggcatca
    27301 gccattaata aagcggtttt ataggcatgt ttgtttttca tgtgtcatcc ccattttaaa
    27361 atagtgagaa ctcccgaccg cagaccgtca tgtttttatg tatgattgtc tgtttaattt
    27421 cctttattcg ttctcagagc atcaacatac tcgtaagcct tttcacaggt tttattcatg
    27481 gaacgaatca gacaaagtaa gaaatcatct gtctctccgt tatcaccaga gtttttaaat
    27541 ataaactcaa gcattgacgt gttttctttt atttctgctg ccacttcctc aagcataatc
    27601 aggggagttt tcatgttctt tgttccttaa atgcatcgca tgcgcttctg gcgtattgtt
    27661 gtgccagtaa aaatatgtca tccgaaagtt catcacattc ttcatcaccg gaagccgaaa
    27721 tgattaaccc cgcttcaagc agtactgcaa tgtgatgaaa agctgtttcc ggttcgttgg
    27781 tgaggccttt gaacattttc atcttacgat tcctttgtcg tgtcagaatg gaaacttgta
    27841 agtaaccaca tcggatcgca gtcaagtata tttgccagag gaatcacctg gcttgccaga
    27901 ggttcagcct tgccgttctc ccactggatg atggtttctt catcaactcc aagcagcgtg
    27961 gcgagttcag cggtggatag accgcagctt tcgcgttggg tgcggatgcc atcacggcaa
    28021 tcttttttgt ttctcggagg taacagaata tgttttagtt gttggatttt ttcgattcca
    28081 acctcaagga gtacgtgacg gtttctttcc gatacagact ccagactttc atgaagcagc
    28141 gattctactt gttcgataag catcaattgt gaagtagaca taaaaaaacc tcgtaaccaa
    28201 tatttcgaaa tctaggttac gagtgagtaa tgtaggtgtc aagagcaatt aatgtttttt
    28261 gttgaattaa atttgcatac gcatctcaac gacaacacca ataatttcac aatccccatt
    28321 gatggggatc agtggatagc gtgggtttag tggctttagg aaacattgcc cagcgtcttc
    28381 tatgtactgt ttaagggttg cctctccgga gtttttgagc ctggcgacga catattttcc
    28441 tgaaaaaact tctttgtcag ggttaacaag aatgttcatg ccttcaaaaa tagagaactc
    28501 tccattctgt gatgtcattg agtccccttt cacttccagc cagaagccgt gatcgcccgc
    28561 atatatatcg ctaccaatcc attcttctgg ataagctatg taatcttcgc agttccaggg
    28621 gccagcactc acccatgtta gtttcggata tctataacgg tttggagaaa ctggcttagg
    28681 atttcggacg ttttgaatgc tatctgtagg tgatggggcg ctgccgtcaa gaagccattt
    28741 aggagagcat ttcaatgcat ccgcgattct aaataggttt gaagatcttg tgtcttgtga
    28801 gtctcctaac tctattttgc tgattgttac acgcgagaca ccagtcaatt ttgccaggtc
    28861 atcttgtgac attcctagct gtactctgcg cattaacagt ctgccaccaa agctatcgtg
    28921 atccatcttc tcccccttct tgtcgaaata atcgtttcga aacaaatata gcaaaaaatc
    28981 ttgaaatcta ggtttcggaa aattaagatt aatcgaaaca aacgtgtcga ggtggatatg
    29041 gatttgtatg aaattctaaa aaacatgttt ggatcgaatg ttgaaatagg tcgttatttt
    29101 ccccgaaggg gaagggctag aaccggacag gctgtaggta agtggaaaac ccgaggtgtg
    29161 ccggaagacg tggcgatcct ttgtcatctg gatcccaaaa ttccttatca gcatccgtcg
    29221 ctcatgaatg ccagtcatga atcgtgatgt tctgccggag gctgtatgtc ccctgattac
    29281 gttcagatgg agatgcctgc caggtacagc caggcagatt cagagtggat taagcggcag
    29341 ttacagagcc tgccttcctc actgagacag aaggttgccc tgaaatacgc ggaggtttac
    29401 gaagttactt ttgattccga gcctgtttca ttccgtaagg agaacagagc gaggcatgaa
    29461 gccaatgtac ggctgcgcag gttcgttgaa acacacgggc gtgcattaca ggggtatacg
    29521 actcagccgc ccctggcagg aacgcaacag cgtacctgaa tggtgttggt cttaaaggtg
    29581 accgacgggc tgagacccca cttctcagga tgcacctg
//
LOCUS       DAZGBC010000051        28657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGBC010000051 DAZGBC010000000
VERSION     DAZGBC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.051"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHK1J_002173"
     CDS             complement(<1..617)
                     /locus_tag="ACHK1J_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7911432.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(688..2802)
                     /gene="fusA"
                     /locus_tag="ACHK1J_002174"
     CDS             complement(688..2802)
                     /gene="fusA"
                     /locus_tag="ACHK1J_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO7911433.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="ACHK1J_002175"
     CDS             complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="ACHK1J_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO7911434.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="ACHK1J_002176"
     CDS             complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="ACHK1J_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO7911435.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="ACHK1J_002177"
     CDS             complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="ACHK1J_002177"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO7911436.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="ACHK1J_002178"
     CDS             complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="ACHK1J_002178"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO7911437.1"
                     /translation="MKRIAFVFSTVPHGTAVGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="ACHK1J_002179"
     CDS             complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="ACHK1J_002179"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO7911438.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="ACHK1J_002180"
     CDS             complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="ACHK1J_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7911439.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="ACHK1J_002181"
     CDS             complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="ACHK1J_002181"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7911440.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6928..7146
                     /gene="slyX"
                     /locus_tag="ACHK1J_002182"
     CDS             6928..7146
                     /gene="slyX"
                     /locus_tag="ACHK1J_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO7911441.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="ACHK1J_002183"
     CDS             complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="ACHK1J_002183"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7911442.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="ACHK1J_002184"
     CDS             complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="ACHK1J_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO7911443.1"
                     /translation="MAIRKRFIAGAKCPVCQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8090..9895)
                     /gene="kefB"
                     /locus_tag="ACHK1J_002185"
     CDS             complement(8090..9895)
                     /gene="kefB"
                     /locus_tag="ACHK1J_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO7911444.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="ACHK1J_002186"
     CDS             complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="ACHK1J_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO7911445.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10577..12490
                     /gene="yheS"
                     /locus_tag="ACHK1J_002187"
     CDS             10577..12490
                     /gene="yheS"
                     /locus_tag="ACHK1J_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7911446.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12490..13512
                     /gene="yheT"
                     /locus_tag="ACHK1J_002188"
     CDS             12490..13512
                     /gene="yheT"
                     /locus_tag="ACHK1J_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7911447.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13506..13724
                     /gene="yheU"
                     /locus_tag="ACHK1J_002189"
     CDS             13506..13724
                     /gene="yheU"
                     /locus_tag="ACHK1J_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO7911448.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13778..14647
                     /gene="prkB"
                     /locus_tag="ACHK1J_002190"
     CDS             13778..14647
                     /gene="prkB"
                     /locus_tag="ACHK1J_002190"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO7911449.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="ACHK1J_002191"
     CDS             complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="ACHK1J_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO7911450.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15408..16040
                     /gene="crp"
                     /locus_tag="ACHK1J_002192"
     CDS             15408..16040
                     /gene="crp"
                     /locus_tag="ACHK1J_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO7911451.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16091..18181
                     /gene="yhfK"
                     /locus_tag="ACHK1J_002193"
     CDS             16091..18181
                     /gene="yhfK"
                     /locus_tag="ACHK1J_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO7911452.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTAPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRIIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKT"
     gene            complement(18248..19468)
                     /gene="argD"
                     /locus_tag="ACHK1J_002194"
                     /gene_synonym="dapC"
     CDS             complement(18248..19468)
                     /gene="argD"
                     /locus_tag="ACHK1J_002194"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO7911453.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     YQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19554..20117)
                     /gene="pabA"
                     /locus_tag="ACHK1J_002195"
     CDS             complement(19554..20117)
                     /gene="pabA"
                     /locus_tag="ACHK1J_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO7911454.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20149..20751)
                     /gene="fic"
                     /locus_tag="ACHK1J_002196"
     CDS             complement(20149..20751)
                     /gene="fic"
                     /locus_tag="ACHK1J_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO7911455.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20741..20908)
                     /gene="yhfG"
                     /locus_tag="ACHK1J_002197"
     CDS             complement(20741..20908)
                     /gene="yhfG"
                     /locus_tag="ACHK1J_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO7911456.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(21013..21585)
                     /gene="ppiA"
                     /locus_tag="ACHK1J_002198"
                     /gene_synonym="rotA"
     CDS             complement(21013..21585)
                     /gene="ppiA"
                     /locus_tag="ACHK1J_002198"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO7911457.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21856..23037
                     /gene="tsgA"
                     /locus_tag="ACHK1J_002199"
     CDS             21856..23037
                     /gene="tsgA"
                     /locus_tag="ACHK1J_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO7911458.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23299..25842
                     /gene="nirB"
                     /locus_tag="ACHK1J_002200"
     CDS             23299..25842
                     /gene="nirB"
                     /locus_tag="ACHK1J_002200"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO7911459.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLIGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25839..26165
                     /gene="nirD"
                     /locus_tag="ACHK1J_002201"
     CDS             25839..26165
                     /gene="nirD"
                     /locus_tag="ACHK1J_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO7911460.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26291..27097
                     /gene="nirC"
                     /locus_tag="ACHK1J_002202"
     CDS             26291..27097
                     /gene="nirC"
                     /locus_tag="ACHK1J_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO7911461.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27116..28489
                     /gene="cysG"
                     /locus_tag="ACHK1J_002203"
     CDS             27116..28489
                     /gene="cysG"
                     /locus_tag="ACHK1J_002203"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO7911462.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6941 a         7701 c         7227 g         6788 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaagaatcc aggaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcata ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcacgagcag caccaccgta ggttttagcc agtacggtgg tgattgcagc ggtcagagta
      541 gttttaccgt ggtcaacgtg gccgatagta ccaacgttaa cgtgcggttt tgtacgttca
      601 aatttttctt tagacacggc tatattcctt actatagtgc tctccccttc aggagagagc
      661 acgggacttt ggtattaacc cttaggctta tttaccacgg gcttcaatta cggcctgagc
      721 aacgttactc ggcgcttcat catacttcag gaattccata gtgtatgatg cacgaccttt
      781 ggtcagagaa cgcagctgag ttgcgtatcc gaacatttca gacagcggta cttcagcgtg
      841 gatcttaacg ccagtaactt cagattcctg acctttgagc ataccacgac gacggctcaa
      901 gtcaccgata acgtcaccgg tgttctcttc cggagtttct acttcaacct tcatgatcgg
      961 ctcaagcaga actggtttcg ctttcttaaa gccttcttta aaggcgatag aagcagccag
     1021 tttaaacgcc agttcagagg agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc
     1081 catgtctact accgggtagc ctgccagcgg acctgctttc agctgttcct ggataccttt
     1141 atcaacggcc gggatgtatt cgccagggat tacaccacct ttaatgtcgt tgatgaactc
     1201 gtagcctttc gggtttgaac ccggctccag cgggtacatg tcgataacaa catgaccata
     1261 ctgaccacga ccaccagact gtttcgcgtg tttaccttca acatcggtaa ctttctggcg
     1321 gatagtttca cggtaagcaa cctgcggttt acctacgttc gcttcaacgt tgaattcacg
     1381 cttcatacgg tcaacgatga tgtcgaggtg cagttcgccc atacccgcaa tgatggtctg
     1441 gttagattct tcgtcagtcc atacacggaa agacgggtct tctttagcca gacggcccag
     1501 agccagaccc attttttcct ggtcagcttt ggttttcggt tcaactgcga tggagattac
     1561 cggctcaggg aattccatac gttccagaat gatcggcgca tccgggtcac acagggtgtc
     1621 accagtggtt acgtctttca gaccgatagc agcagcgatg tcgcccgcgc gaacttcttt
     1681 gatctcttca cgtttgttag cgtgcatctg aacgatacga ccgaaacgct cacgtgcagc
     1741 tttcacggag ttcagtacgg tatcaccaga gttaaccaca ccggagtaaa cacggaagaa
     1801 ggtcaggtta ccaacaaacg ggtcggtagc gattttgaac gccagtgcag agaacggctc
     1861 gtcatcactt gcgtgacgtt cagccggagt gtctttaccg tcgtccagga taccgttgat
     1921 cgcaggtacg tcaaccgggg atggcaggta atcaattacc gcatccagca tcgcctgaac
     1981 acctttgttc ttgaacgcag aaccacaggt taccaggatg atttcgttgt tcagaacgcg
     2041 ctgacgcaga gcacctttga tttctgcttc agtcagttct tcaccaccca ggtatttttc
     2101 catcagctct tcagaagctt cagctgcgga ttcgatcagg ttctggtgcc attcgttagc
     2161 cagttcaacc atgtctgccg ggatatcttc gtattcgaag gttacgccct ggtcagcgtc
     2221 gttccagttg atagctttca ttttcaccag gtcaacaaca cctgtgaaat gttcttcagc
     2281 accaatcgcc agctgcagcg gaaccgggtt cgcgcccaga cgggttttga tctggttaac
     2341 aactttcagg aaattcgcac ccatgcggtc cattttgtta acgaacgcaa tgcgcggaac
     2401 tttatatttg tttgcctgac gccatacggt ttcagactgc ggctgaacac caccaactgc
     2461 gcagtaaacc attaccgcac catcgagaac acgcatggaa cgttctactt cgattgtgaa
     2521 gtcaacgtgc cccggggtgt cgatgatgtt gatgcgatgc ggctcatact gcttagccat
     2581 accagaccag aatgcagtag tcgcagcgga agtgatggta ataccacgtt cctgctcctg
     2641 ctccatccag tccatggttg cagcgccgtc atgaacttca ccgattttat ggtttacacc
     2701 ggtgtagaac agaatacgtt cggtagtagt ggttttaccg gcgtcgatgt gcgcactgat
     2761 accgatgtta cggtagcgtg cgatgggtgt tgtacgagcc atttgtttcc tcgtttatct
     2821 tttaggcgtt caatttaagt agcccaaagc gggctgctta ctggaagcgc ccgcctggtg
     2881 actaaaactc cgaagggatt accaacggta gtgtgcgaac gccttgttgg cttcggccat
     2941 acggtgaacg tcttcacgtt tcttaactgc agtacctttg ttttctgcag catcagaaag
     3001 ttcgttcgcc aggcgcagag ccatggattt atcaccgcgt ttacgagcag cttcaacgat
     3061 ccaacgcatt gccagagcat tacgacgaac cggacggact tcaactggta cctgataagt
     3121 agaaccacca acgcggcgag acttaacttc tacagtcggg cgcacgtttt cgagagctac
     3181 ttcgaatgct tccagttcag atttaccaga gcgctgagcc agggtctcca gcgcgctgta
     3241 tacgatagat tcagcagtag attttttacc atctaccatc aggatattta caaatttagc
     3301 cagcagttct gatccgaact tcggatccgg cagaatttta cgctgaccaa tgacgcgacg
     3361 acgtggcatg gaaatactcc gttgttaatt caggattgtc caaaactcta cgagtttagt
     3421 ttgacattta agttaaaacg tttggcctta cttaacggag aaccattaag ccttaggacg
     3481 cttcacgcca tacttggaac gagcctgctt acggtcttta acgccggagc agtcaagcgc
     3541 accacgtacg gtgtggtaac gaacacccgg gaggtcttta acacgaccgc cacggatcag
     3601 gatcacggag tgctcctgca ggttgtgacc ttcaccaccg atgtaggaag tcacttcgaa
     3661 accgttagtc agacgaacac ggcatacttt acgcagcgcg gagttcggtt ttttaggagt
     3721 ggtagtatat acacgagtac atacgccacg tttttgcggg catgcttcca gcgcaggcac
     3781 gttgcttttc gcaactttgc gagcacgtgg tttgcgtacc agctggttaa ctgttgccat
     3841 taaatagctc ctggttttag cttttgcttc gtaaacacgt aataaaacgt cctcacacaa
     3901 tatgaggacg ccgaatttta gggcgatgcc gaaaaggtgt caagaaatat acaacgatcc
     3961 cgccatcacc aggccatctg gctggagtgc ttaaccgtaa gtctgacgaa atcagtatag
     4021 tcaatgggaa tgatgtcgtt cgaaatttga ccagtcaaac cgcgggcaat aaggtcttcg
     4081 ttcagggcat agaccttaat gggggcatta cgcagacttt caaggtagcg gttaccgtca
     4141 actgcggcag ttacgccatc ttgcaataat agcagttcgt cgccttcact gagcagacgc
     4201 agcagcgcag caaaatccgt cagccagggt gaacgatgta acgtgtgcag cataaacgcc
     4261 tcaaaacctc aaaataacat cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag
     4321 cggcgtggct tcgacaacaa agggggtctg cggatctaac ccgcgttcgc gcagtgaagc
     4381 cgcacaaacc cagcactgtt caatgtcgta cagacccaac aatttaaaag tggcaatgta
     4441 atcacgcgcc agcactgcat cgggcttttg tcctggcagt agctgaaaaa cgccatcagc
     4501 aataaagaag acggccagct cgtcagttaa cgcggaagtt gccagtaaag catctaaacc
     4561 ttcccgcccg acagcggtac catgaggcac agtagaaaaa acaaacgcaa ttcgtttcat
     4621 cagaactgta ccaccctgtc acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt
     4681 aaggtaaatc cctgctgaag gtttgacgaa gccagcccca gtcttccggc ctccgtttca
     4741 tcaacaacgc cacggcgtaa tgctgccgct acgcagatat tcagcgccac accatgttgc
     4801 gcattcaact gttgccagcc gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcaag
     4861 tggttagcgt tatagacccc ttcccgatag aaaaagacgc tgcttaactt atggccttct
     4921 gctatcagcg cctgcgcaaa ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc
     4981 ccggtcacca cgatggcaaa acgcattact tatcttgccc ctggaaatca ccgctcttga
     5041 actggcggat gtagagatag acagtgtgtt tggagatgtt caggcggtca gcaacctggt
     5101 tgatcgcatc tttaatatcg aagatccctt tctcgtagag attcagcacg atctgacgat
     5161 ttttggcgtt attagaaaca ttgcgatcgg cattcacttc ttcgatggtg aactccagcg
     5221 tttgggtaac cagatcttca acagaagagg caaagttgac gcttgaaccg acatccgggg
     5281 tttctggcgg cacaaaggtg ctcataatct gcgagaaggg aacatcaaga ttcatattga
     5341 tgcacagcag accaattaca cgctgttcgc ggttacgaat cgcgatagtc agggacttca
     5401 ttaatacgcc gcttttggcg cgagtaaagt agcatttaga aacgctgcta tccgctcccg
     5461 tcatatcgtg cagcatacgt agcgccaggt cagtaattgg cgaaccaatc ttccggcctg
     5521 tatgttcacc gttagcaatg cgaatggctg aacattttag atcctgcaaa gagtgcaaaa
     5581 cgatttcaca gtgggagcca ataagcatcg ctaacccgtc caccaccgct tcgtaggatt
     5641 tcagaatatc aaaatcggtc tggtcgaaag gacgttgatc cagtaaatcc aactcactgg
     5701 tttcgttggt taaaagcgac ctggacatga aaaaaaacac tccttttcag gagcctgtcg
     5761 ttaacttttc agggcaggct cattaatgat gcgggtaact aaattaatac agcggaggtt
     5821 ccgctttcca gcactaatta tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg
     5881 gcggttctta atgcttattt tttagcagaa tctgcggctt tcgcatcagc ttccggcttt
     5941 gcatcagcct tcggcgctgg tttcacatcc agcagctcta cgtcaaacac cagggtagaa
     6001 ttcggtggga tccccggaac acccgctttg ccgtaagcca gttctggtgg aataaccagt
     6061 ttgatcttac cgcctttctt gatgttcttc agaccttctg tccaacccgg gataacaccg
     6121 tccagacgga aagaaagcgg ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg
     6181 atcagcgtac ctttgtagtt cactacaaca gtatcgctgt ctttcggtgc ttcgccttta
     6241 ccggcttcta ctacctgata aaccagacca gttgaagagg ttttcacacc tttctctttg
     6301 gcaaatttct cgcggtactc tttacctttt gcttcgttat cagccgcgtc tttttccatc
     6361 ttcgcctgag cagaagactt cacgcgagct tcgaatgctt gcagagtctg ttcgatctct
     6421 tggtcggaga gtttgctctt atcagcaaat gcatcctgaa caccagcgat cagctgatct
     6481 ttatccagtt tgatgcccag tttttcttgt tcttttagag agttttccat gtaacgaccc
     6541 agcgaggcac ccagtgcata agctgatttc tgatcgtcat ttttgaacgc tgctttgctg
     6601 tcagcagttg tagcaggttt tgcagcttca gcagcaaaag tgattggtgc atgcagggca
     6661 acggccattg tggtcgccag cagcgttact ttaaacagtg atttcatcca tatctccagg
     6721 atcggggcat ctcaccccag ggttaactat tatcagaagg gtactataaa gcgttgtcga
     6781 acaaatctac atacagacac gccctattat catctatttt cagactcttt ttgtttaaat
     6841 tagtttcgat gaccgcgaaa tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg
     6901 gcgaccattc gacaaaagag gtgaatcatg caggatttat cattggaagc acgcctggca
     6961 gagctggaga gccgactggc ttttcaggaa atcaccattg aagaactgaa cgtcacggtg
     7021 accgctcatg aaatggagat ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag
     7081 ttaaaagcca gccagccgtc gaacatcgcg tcgcaggctg aagaaacgcc accgccacat
     7141 tattgaggcg taaaaaaagc ggggattccc cgcttttttg tcactttttc ggtattagtg
     7201 gcaaccgcaa ccgccgttac ctttaccgcc acagcagcct tcgccaccgt gctcatgacc
     7261 gtgatcatgg ccatgaccac cgcagcaacc gtcgtggtcg tgatcgtggt gatgatcgtg
     7321 cgcaccgtga acgtggccat gagccagctc ttcttcagtc gcttcgcgaa tcgccacaac
     7381 ttcaacgttg aatttcaggt tctgaccggc cagcatgtgg ttaccatcaa ccacgacgtg
     7441 atcgtcttca accgcagtga tttcaaccgg taccggaccc tggtcggttt cagccaggaa
     7501 acgcatacct acctgcagtt catcaacgcc cataaatacg tctttaggca cacgttgcac
     7561 caggttttcg tcgtactgac cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc
     7621 gccaacttca tgaccttcca gcgccgtttc caggccagag atcagggaac cgtgaccatg
     7681 caggtagtcc agcggcgcac tcaccggaga ctcatcaacc aacacaccgt cttctgtacg
     7741 tacctgatag gccaggctga ccaccaggtc ttttgctact ttcatgatat ctcctgagca
     7801 tgggaagaat agtggcgcag attgtagcgg aattctgcag ccgtgtactc actagcttaa
     7861 aaaaactcgg cgcatatcgc tagtccggat gaaaaatccc gatcacttgc tcatctttgc
     7921 gaacgtgatc gcgagcttct ttgtctgctt ctcgcatctg atgtccgcac ttaacacatt
     7981 caacaatatc aatattattt tcgcgccaca tcgccattga atcctgcgcc tgacagaccg
     8041 ggcattttgc gcccgcaata aaacgttttc ggattgccat ctttaccctc tactcaaatt
     8101 catcccagcc gtccagctgg cgtcgttctt gttgcatttc acgctggaaa atctcttcca
     8161 gttcgcgtcg ggcttccctg gcgcgagaaa tttgtacggt atcggtatgc attgggatga
     8221 gctctcgcag catccgcata tccaggcggc gaaaatgcag ttgcgcccgc tgtgcctgat
     8281 gcggatgcat accaagcgtg accagcgtct tgcgccccag ctctaacgca ctggagaatg
     8341 tttcacggga aaactgcgtc acccctgcct gtaataactc atgcgcttcc acacgtccgc
     8401 gcgctcgcgc aagaatatgc aaatgcggaa agtgctgctg gcatatttcc accagcttca
     8461 tggtgtcttc cggctcgtta caggtaatga cgatagactc agcggcctct gcacccgcag
     8521 aacgtaaaag atctacctgc gtggcgtcgc cgtaataaac tttgtagcca tatttgcgca
     8581 tcaggttaac ggcgctgata tcccgctcca gcacagtaat gcgcatttta tttgccatca
     8641 acaaacgacc aatcacctgc ccaaaacgtc caaagcccac gacaatgacc tggggtttat
     8701 catcgttgac ccacggtttt tcgtcttctt cttccggtcc gttaaactgg cgggatagcc
     8761 atttatccac cagcttcatc agcagcggcg tggtcatcat ggaaagcgtc accgtcacca
     8821 gcaacagcgc catctggtcg ccctggaata agcgttgtga agaagcggta gaaaagagga
     8881 caaaggcaaa ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tctgagctac
     8941 gcacgccata caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa
     9001 ccacgcttat cactacccac aacagatggg tataaagcac cccgaggttg agtgacatcc
     9061 cgacagagat aaagaacaaa ccgagcagca agcctttaaa gggatcgata gccgtttcca
     9121 gttcatggcg atattcactt tccgccagca gcacgcctgc aataaacgta ccgagcgcca
     9181 tcgacagccc caacgcatcc ataaacaatg cggaacccaa taccagcagc agcgtcgcgg
     9241 cggtgaacac ttcccgcacg ccagaagctg caataaaacg gaataccgga cgcaataaat
     9301 agcgcccgcc aatcagcatg ccgacaaacg ccagcacctt catgccgatc ttcatccagt
     9361 cgaaatgttc gtctgccgaa cccgccaaca acggcactaa cgccagtgcc gggatcaccg
     9421 ccagatcctg aaacagcagt accgaaaacc cgagctgccc ggattcgctg cgattcatcc
     9481 ctttctcacg catcaattgc aacgccattg cggtcgaaga catcgcaagg ccaatgccac
     9541 cgaccaccgc cgcctgccag gcgaaatccg tcagcatcaa taatcccgcc agcaacgccg
     9601 cgcttaacag cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagct
     9661 tggagggatt caactcaagg ccgatgataa acatcaggaa caccacgccg agttccgaaa
     9721 agtggaggat ctcatcgacg tcgctaataa accccagccc ccacgggcca attgcaatcc
     9781 ctgccagcaa atatcccaac acagcgccaa tacccagccg cgatgccaac ggcaccgcag
     9841 ccaccgccgc gaagagaaac agcactcctg caagtaaaaa atcggaacct tccatcagcg
     9901 gcctcctgga gacagcggat ttgccagcca gtcaccgtag gctctggcgt ggctcgccag
     9961 ctcctgtgcg ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg
    10021 gcacatgccc gccgccagtt caaaggggcg caggacatcg ctcatcgggt agcgattcaa
    10081 cgcgtcataa cggtaagcac tttccggctc gccggtggta atcacactac gccagtactt
    10141 tcccgccagt tggtttcctc ccggcccgct ggcaaaacca cggcttaata cccggtccag
    10201 ccactctttc agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac
    10261 ctcgtgctcg cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg
    10321 cgcgtaaagg tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg
    10381 gtttgccacc gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg
    10441 agacatcatc cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg
    10501 cgcccggtgc ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct
    10561 gaacatacgg caccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt
    10621 cctgctggat aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa
    10681 aaacggctgt ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg
    10741 cggcagctac acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccggc
    10801 gttaccgcaa gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga
    10861 agcgcagcta cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatgg
    10921 caagctggat gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg
    10981 cctcggtttc agcaatgaac aactggagcg cccggtaagt gatttctctg gtggctggcg
    11041 tatgcgtctt aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga
    11101 accgactaac cacctcgatc tcgatgccgt tatctggctg gaaaaatggc tgaagagcta
    11161 tcagggcacg ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgtcgataa
    11221 aattattcat atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga
    11281 agtacagcgc gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg
    11341 cgtagcgcat ctgcaaagtt atatcgaccg tttccgtgcc aaagccacca aagcgaagca
    11401 ggcccagagc cgcattaaga tgctcgagcg tatggagctg attgcccccg cgcacgtcga
    11461 caacccgttc cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat
    11521 ggaaaaggtc agcgcaggct atggcgatcg cattattctc gactcgatta aactgaatct
    11581 ggtccccggt tcgcgcattg gtctgttagg ccgcaacggc gcgggtaaat cgacattaat
    11641 caaactgtta gccggtgaac ttgcgccagt cagtggtgaa ataggcctgg caaaagggat
    11701 caagctcggc tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcgcctat
    11761 tcaacatctg gcacgtttag cgccgcagga actggagcaa aaactgcgtg actacctcgg
    11821 cggctttggt ttccagggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga
    11881 aaaagcccgc ctggtgctgg cattaattgt ctggcagcgg ccgaatctgc tgctgctcga
    11941 cgaaccgact aaccaccttg acctcgacat gcgtcaggca ctcaccgaag cattaatcga
    12001 gtttgaaggc gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga
    12061 cgacctctac ctggttcacg atcgtaaagt cgagccgttc gacggcgatc tggaagatta
    12121 tcaacagtgg ttgagcgacg tacaaaagca ggaaaaccag accgacgacg cgccaaaaga
    12181 gaacgcgaac agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agttgcgtgc
    12241 gcaaacccag ccactgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa
    12301 cgcgcaactg gcgcaggcag aagagaaact cggcgacagc gaactgtatg accagagccg
    12361 taaagcggag ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga
    12421 gtgcgaaatg gcatggctgg aagcccagga gcagcttgag cagatgttgc tggaaggcca
    12481 aagcaactga tggcgcagat aacgacgacc gatgccaatg aattcagcag cagtgctgaa
    12541 ttcaccccta tgcgcggctt tagcaattgt catctgcaaa ccatgctgcc gcgtctgttt
    12601 cgtcgcaagg tgaaattcac cccgtactgg cagcggctgg agttgcccga cggcgatttt
    12661 gtcgatctcg cgtggagtga agccccagca caggcgcggc ataaaccgcg tttagtggta
    12721 tttcacgggc tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg
    12781 caaaagcgcg gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac
    12841 cgtatgcacc gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg
    12901 ctgcaacgcg aatttggtca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat
    12961 atgctggcct gtttgctggc aaaagaaggc aatgatctcc ctgttgatgc ggcggtgatt
    13021 gtctctgcgc cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc
    13081 gtttatcagc gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc
    13141 taccccggaa cactgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa
    13201 tttgacgatc tcatcaccgc cagaattcac ggctacgctg acgctatcga ctattatcgt
    13261 cagtgtagcg ccatgccgat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc
    13321 aaagacgatc cgtttatgga tcatcaggtg atcccgaaac cggaaagtct ccccccgcag
    13381 gtggagtatc aactgactga acatggcggt catgttggct ttattggcgg tacattactt
    13441 catccgcaaa tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg
    13501 aaatcatgtt gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa
    13561 gctttgtgtt acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag
    13621 tcgccgacgt caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc
    13681 acgaaacggt caatattatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg
    13741 gacgagataa taatgatagc taagtcatgg agttactatg tctgccaaac atccggtcat
    13801 tgcggtaaca ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat
    13861 attcgcgcag ttaaatctgc atgcagccga ggtggaaggc gacagttttc accgttacac
    13921 ccgcccggaa atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta
    13981 cttcggcccc gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca
    14041 gagcggcaaa gggaaatctc gcaaatatct gcatacctac gatgaagccg taccgtggaa
    14101 tcaggtaccg ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt
    14161 ttatgaaggt ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga
    14221 cttactggtc ggcgtggtgc ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga
    14281 caccagcgag cgcgggcact cacgagaagc agtgatggac tcagtagtgc gttcaatgga
    14341 agactatatc aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt
    14401 tcccaccgtc gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag
    14461 ctttgtggtg atccattttc gtaatctgga agggatcgat ttcccctggc tgctggcgat
    14521 gttgcaaggc tcattcattt cccacatcaa tacgttagtg gtacctggcg gcaaaatggg
    14581 gctggcaatg gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat
    14641 cgagtaactt tgctattatg ccggataaaa tactatccgg cttcacaacg ggatagttaa
    14701 atcacgcggc aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg
    14761 ccactgaaca atatttctca gcagagagat caaccgcacg cgcaaccgct gcgtctttca
    14821 ggtcgcgacc ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt
    14881 cgcggcgttc agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca
    14941 ggatagatac cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg
    15001 gacttggtgc tttatcgcct gagttgccgt ccattaaaat ctgatgacca gaggcggatt
    15061 cgcccagaaa agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac
    15121 tgcgtcaatt ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct
    15181 gggtcatgct gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag
    15241 gatgctacag taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca
    15301 gtacagttga tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc
    15361 ggcgttatct ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca
    15421 aaccgcaaac agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc
    15481 catccaagag cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta
    15541 aaggctctgt ggcagtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc
    15601 tgaatcaggg tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg
    15661 catgggtacg tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc
    15721 aattgattca ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc
    15781 tgcaagtcac ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg
    15841 cacagactct gcttaatctg gcaaaacaac cagacgctat gactcacccg gacggtatgc
    15901 aaatcaaaat tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg
    15961 gacgcattct gaagatgctt gaagatcaga acctgatctc cgcacacggt aaaaccatcg
    16021 tcgtttacgg cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt
    16081 gtttcccccg atgtggcgca gactgattta tcaccccgat attaactatg cactacgaca
    16141 aacgctggtg ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg
    16201 tctgctcttc tctctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa
    16261 acgttttttc aaacgcttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac
    16321 acagctgcta ctggcaaaag atgttcccct gccctttttg ctgaccggat taacgctggt
    16381 acttggcgtc accgctgagt tggggccatt gcacgcaaaa ttgcttcccg catcgctgct
    16441 ggcagccatt tttaccctca gtctggcggg atacatgccg gtctgggaac cgttgctcat
    16501 ctatgcgttg ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg
    16561 cgaacaaccg ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga
    16621 agccaaatac agcctgctta cccagcacac cgcccctgaa aaagcgctgc cgccgctgct
    16681 ggtgcgccag caaaaagcgg tcgatctaat tacccagtgc tatcagcaaa tgcatatgct
    16741 ttccgcgcaa aataatactg actacaagcg gatgctgcgt attttccagg aggcgctgga
    16801 tttgcaggaa catatttcgg tcagtttgca tcagccggaa gaggtgcaaa aactggtcga
    16861 gcgtagccat gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg
    16921 cgtgctggct gatgacattc tttaccatcg cctgccaacg cgttttacga tggaaaagca
    16981 aattggcgca ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg
    17041 ctattggcac ttcagccgca ttgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg
    17101 tgacttactg gccgataaac agcgacgaat gccgttactt ccggcgctga aaagttatct
    17161 gtcactaaag tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc
    17221 cagcttgatg ggcaccgcgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt
    17281 gctggtgacg caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg
    17341 aaccgtggtc gggttaatca ttgcgggcgt ggcgctgcac tttaaaattc ccgaaggtta
    17401 caccctgacc ttgatgctga ttaccaccct tgccagctac ctgatattgc gcaaaaacta
    17461 cggctgggcg acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct
    17521 gaacggtgag cagtacatcc ttccgcgtct tatcgatacc attattggtt gtttaattgc
    17581 tttcggtggt accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc
    17641 ccatgacgct ttagaagcct atcaggaagc gattcgcttg attctgagcg aggatccgca
    17701 accaacgcca ctggcctggc agcgaatgcg ggtaaatcag gcgcataaca ctctgtataa
    17761 ctcattgaat caggcgatgc aggaaccggc attcaacagc cattatctgg cagatatgaa
    17821 actgtgggta acgcacagcc agtttattgt tgagcatatt aatgctatga ccacgctggc
    17881 acgggaacac cgggcattgc cacctgaact ggcgcaagaa tatttgcagt cttgtgaaat
    17941 cgccattcag cgttgtcagc agcggctgga gtatgacgaa ccggggagtt ctggcgatgc
    18001 caatatcatg gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca
    18061 gcatttacag cggattattg gtcatctgaa caccatgcac acaatttcgt cgatggcatg
    18121 gcgtcagcga ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaagacgta
    18181 atacaggcta aaccatcgga tgaggtgttc acgccgcatc cgacatcttt ttcctgatgc
    18241 gtcttattta cgccccaaca actttcgcca ccgcgtgggc gaaacgttgc atcccttcat
    18301 cgatatccgc atcttccacc accagcgacg gtgcaaaacg catcacatcc ggtccggcat
    18361 tcagcaccat tacgccagcc tctgcgcccg catacaggaa atcacgcgcc cgacctttgt
    18421 actgtggctt aagctccgcg ccaatcaaca gccccatacc gcgaatatcg ctaaacacat
    18481 cgtactgctg atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc
    18541 cttccagcac ttcaggtgta ttgatgatat caaacgccgc ccccgctact gcacaggcca
    18601 gaggattacc gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg
    18661 ccgtggtcag catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa
    18721 tatccggcgt cacgccgtag tgcatgtaag caaacaaatc gccagtccgc cccatcccgc
    18781 actgcacttc atcaaacacc aataatgcct gatattgatc gcacagctcg cgcaagccct
    18841 gcaaaaactc tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca
    18901 ccgcacaggt gtgatcatcc atcaccgctt tcactgcgtg gagatcgtta aagggaacgt
    18961 ggatgatgtc tgctggtttt ggcccaaagc cgtcggaata ttttggctgc ccacccaccg
    19021 aaacggtaaa cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga
    19081 acgggctatg acgcacacag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt
    19141 ccgtgccaga gttcataaac accacacgtt cggcaaacgt tgcctcaatc agtttacgcc
    19201 caagacgcag cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct
    19261 gggtttttaa cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc
    19321 cacccgcgaa atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt
    19381 ttaccggaat gaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc
    19441 gtgtaattgc tgtttgttca attgccatct catgatcacc ctgttacgca taaataaatg
    19501 tgaaattata atcacaaaat atgcataaaa aatcattgaa tggcaatcag aaatcagcga
    19561 tgtaggaaat tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaac
    19621 tgcacacctt ccagatccca ctggcgatgg cgaatcccca taatctcgcg ggtttcgctc
    19681 caggccgtca cgtcaaagca cgcaggtaat gagtcaggtt ccaccaccag cgaatgatag
    19741 cgtgtcacag taagtggatt tgccagcccc cgaaatacgc cctcaccgct atgtgtaatc
    19801 ggcgaggttt tgccgtgcat gacctttgca gcgcgcacaa ctttaccgcc aaatgcctgc
    19861 gccattgcct gatgaccgag gcagacgcca agaatcggca agcgcccggc atagtggcga
    19921 ataacgtcaa gggagatccc ggcttcatct ggcgtacagg ggccgggtga gatgacaatt
    19981 ttttgtggtt taagggcgtc gatatccgcc agcgtcaacg catcgttgcg cttaaccagc
    20041 acatccgccc ccagttcaca aaaatactgg tagaggttcc aggtaaaaga atcgtagtta
    20101 tctataagca ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc
    20161 cccggcttcg cttactactt tgctaaatat catctgcagt gcggtgagat cccccattgc
    20221 cccactctga tttgcctgat tccaggcctc tttttcaata ccctgccagc tcagttgata
    20281 tccggcatga atcgccagtt gctcgaagaa gatccgctgt gccagaccac ttcccacccg
    20341 gaagggatgc agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgc
    20401 tttctccagg ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc
    20461 tttttcgata taagcaaagt ggcagaacgg cgtatcgccc tgataaatat caacttcacg
    20521 cagttgccct gcccagtcga aaatatcctg atacagctgg cgatggatag tgcgcaaatg
    20581 cggtaaaccg cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg ccgtcatttc
    20641 gtaagcggcc tgttccagcc gctgctgctg gcggatgttc agccggttac gcatgatatc
    20701 aaggcctgga taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgcctcc
    20761 tcagctcttc aaggcgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga
    20821 cgccttcaag acggcgactg gcctggaaat tacgattacg ctgaagctcc cagagacggg
    20881 acttttgctt atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac
    20941 aagtataagc ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg
    21001 cgcgagaaat cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac
    21061 attctggtac ggaccaacgt catgagtcgg cacctgggaa atcttatcgg caacgtccat
    21121 gcctttcacc actttaccaa ataccgcgta accgaaatca cgctgaccat ggtcaaggaa
    21181 ggcgttatcg gcaacgttga taaagaactg gctggtggcg ctgtctttgt cggcggtacg
    21241 cgccatcgcg atggtaccac gcgtgttgcg caggccgtta tcggcttcat ttttgattgg
    21301 cgggtttggt tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc
    21361 aggaatgacg cggtgaaagg tagtattgtt ataaaaaccg ctgttcacat aatcgacaaa
    21421 gttctgcaca gacactggcg ctttttgttt atccagctcc agttcgatgt taccagctga
    21481 ggttgtcaac aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga
    21541 aagagcgaaa acagcagcca tcgccgccag ggtcgatttg aacatgagat ttccttaaca
    21601 aacagagcag aaaaaaagca agtgaagaga ttctaaatag ccttcaagaa caaggccatc
    21661 cctttaccta attttacgta tctgaaatat atgtaacctg acgatcactt tttattccgt
    21721 gatcaaaatc acctcttaaa atgcaattta gcaaccgatt gcaataaaac atttaaacag
    21781 atcacaaaat cacctaaaat cgcccgctcg cggcatatcc cgctatgcca ttttattttt
    21841 gaacgcaggc caatcatgac taacagcaat cgcatcaagc tcacatggat tagctttctc
    21901 tcctacgcac tgactggtgc gttggttatt gtcaccggga tggtgatggg aaatatcgcc
    21961 gattatttca atctgcctgt ttccagtatg agtaacacct tcaccttcct caacgccggc
    22021 attttaatct ctatattcct caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag
    22081 ttacgttttg gctttctcct gatggtgctg gcggttgccg gtttgatgtt cagccacagc
    22141 ctggcgctgt tctcggcggc gatgttcatt ctcggggtgg tcagcggcat caccatgtcg
    22201 attggtacat tcctggtaac acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta
    22261 tttaccgact ccttcttcag tatggctggg atgattttcc caatgatcgc cgcgtttcta
    22321 ctggcgcgca gcattgagtg gtactgggtt tatgcctgca tcgggctggt gtatgtcgct
    22381 atttttattc tgactttcgg ctgtgagttc ccggcgctgg gcaaacatgc gccaaaaacg
    22441 gatgctccgg tagcgaaaga aaagtggggg atcggcgtac tgtttctctc tgttgcggcg
    22501 ctgtgctaca tcctcggtca gttaggtttt atctcctggg tgcctgagta tgccaaaggc
    22561 ctgggcatga gcctgaacga cgcgggcacg ctggtgagta acttctggat gtcatacatg
    22621 gtcggcatgt gggcgttcag ctttattctt cgcttctttg atttgcaacg cattctgacc
    22681 gtactggctg gtctggctgc gattctgatg tacgtcttta acaccggaac accggcacat
    22741 atggcgtggt caattctcgc cctgggcttc ttctccagcg cgatctatac caccatcatc
    22801 accctgggtt cacagcagac caaagtaccg tcgccaaaac tggttaactt tgtcctgacc
    22861 tgcgggacca tcggtactat gttgaccttt gtggttaccg gcccgattgt tgaacatagc
    22921 ggtccgcagg cggcactgct tacagcaaac ggtctgtacg ctgtcgtctt tgtaatgtgc
    22981 ttcctgttag gttttgtcag ccgtcaccgt cagcataaca ccctgacctc tcattaattg
    23041 ctcatgccgg acggcactat cgtcgtccgg ccttttcctc tcttcccccg ctacgtgcat
    23101 ctatttctat aaacccgttc attttgtcca ttttttgcac aaacatgaaa tgtcagacaa
    23161 ttccgtgact taagaaaatt tatacaaatc agcaatatac cccttaagga gtatataaag
    23221 gtgaatttga tttacatcaa taagcggggt tgctgaatcg ttaaggtagg cggtaataga
    23281 aaagaaatcg aggcaaaaat gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc
    23341 ggccatcgct ttatcgaaga tcttcttgat aaatctgatg cggccaactt tgatattacc
    23401 gttttctgtg aagaaccgcg catcgcttat gaccgcgtac acctctcgtc ttacttctct
    23461 caccacaccg ccgaagagct gtcgctggtg cgcgaaggct tctacgagaa acacggcatc
    23521 aaagttctga tcggcgaacg cgctatcacc atcaaccgtc aggagaaggt gattcactcc
    23581 agcgccgggc gcaccgtttt ttatgacaag ctgatcatgg cgaccggttc ctacccgtgg
    23641 atcccgccaa tcaaaggttc tgatactcag gactgctttg tctatcgtac tattgaagac
    23701 ctcaacgcca ttgaatcctg cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc
    23761 ctgttaggtc tggaagccgc tggcgcgctg aaaaacttag gtattgaaac ccacgttatc
    23821 gaatttgccc ctatgctgat ggcggaacag cttgatcaga tgggtggcga gcagctgcgt
    23881 cgcaaaatcg aaagtatggg cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg
    23941 caggaaggtg tcgaagcgcg taaaaccatg cgttttgccg acggcagcga actggaagtc
    24001 gactttatcg tcttctctac cggtatccgt ccgcgcgata agctggcaac ccagtgtggt
    24061 ctggacgttg ctccgcgtgg gggtattgtt attaatgatt cctgccagac ttccgatccg
    24121 gatatctacg ccatcggtga atgcgcaagc tggaacaacc gtgtatttgg tctggtagca
    24181 ccaggctaca aaatggcgca ggtcgccgtt gaccatattc tcggtagcga aaacgccttt
    24241 gaaggtgctg accttagcgc caagctgaaa ctgctgggcg tagacgtagg cggtattggt
    24301 gatgcgcacg gtcgcacacc aggcgcacgt agctacgttt acctcgacga aagcaaagag
    24361 atctacaaac gccttattgt cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg
    24421 ggcgatacca gcgactacgg caacctgctg caactggtgc tgaatgctat cgagctgccg
    24481 gaaaacccgg attctctgat tctgccggcg cactcgggta gcggtaagcc gtctatcggt
    24541 gttgataaac tgccggacag cgcgcaaatc tgctcctgct tcgacgtcac caaaggtgat
    24601 ctgattgctg ccatcaacaa aggctgtcac accgttgcgg cgctgaaagc cgaaaccaaa
    24661 gcgggtactg gctgcggtgg ctgtatcccg ctggtcactc aggtactgaa cgcggaactg
    24721 gcgaaacagg gcatcgaagt taacaacaac ctgtgcgaac actttgctta ttcgcgtcag
    24781 gaactgttcc atttgatccg cgttgaaggc attaaaacct tcgaagaact gctggcgaaa
    24841 cacggcaaag gttacggttg tgaagtttgt aaaccgaccg tcggttcgct gctggcctcc
    24901 tgctggaacg aatacattct gaagccggaa catactccgc tgcaggattc taacgacaac
    24961 ttcctcgcta acatccagaa agacggcacc tactcggtga tcccgcgctc tccgggcggt
    25021 gaaatcaccc cggaagggct gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac
    25081 accaaaatca ccggctccca gcgcctggcg atgtttggcg cacagaaaga cgatctgccg
    25141 gaaatctggc gtcagctgat tgaagccggt ttcgaaaccg gtcatgccta tgcgaaagca
    25201 ctgcgtatgg cgaaaacctg cgtgggtagc acctggtgcc gctacggcgt gggcgacagc
    25261 gtgggcctcg gcgtggaact ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg
    25321 aagttcggtg tctccggctg tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt
    25381 attatcgcca ctgaaaaagg ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg
    25441 cgtcatgcgg atctgctggc ggcggatatc gatcgcgaaa cgctgatcaa atatctcgac
    25501 cgcttcatga tgttctacat ccgtaccgcc gacaaactga cgcgtaccgc gccgtggtta
    25561 gaaaacctcg aaggcggcat cgattacctg aaagcagtga tcattgacga caaactgggg
    25621 ctgaacgcac atctggaaga agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg
    25681 actgaaacgg tcaatacgcc gtctgcgcag actcgcttca aacacttcat caacagcgac
    25741 aaacgtgacc cgaacgtaca gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg
    25801 tatgaacgta tcccggtaac tctggtggag gacaacgcat gagccagtgg aaagacatct
    25861 gcaaaatcga tgacatcctg cctgaaaccg gcgtctgcgc gctgttaggt gacgagcagg
    25921 tcgcgatttt ccgcccgtat cacagcgatc aggtgtttgc gatcagcaac atcgacccgt
    25981 tcttcgagtc cagcgtgcta tcacgcggac tgattgcgga acaccagggc gagctgtggg
    26041 tcgccagccc gctgaaaaaa cagcgttttc gcttaagcga cggcttgtgc atggaagacg
    26101 aacagttttc cgtcaaacat tacgaagcgc gagtgaaaga cggcgtggtg cagctgcgcg
    26161 gttaatgttt taacgggagg cgcaatgcct cccctttttg catggtcctg taataatctt
    26221 cggtatattg caggacattt tttaaacttt ttgttttatt ttttgttttt attttttaaa
    26281 ggataatcaa atgtttacag acactattaa taagtgtgcg gctaacgctg cgcgcattgc
    26341 acgcctgtcg gcaaataacc cgctcggctt ttgggtcagc tccgccatgg cgggcgcgta
    26401 tgtgggtctt gggatcatcc tgatttttac gctcggtaat ttgctcgatc catccgtacg
    26461 ccctctggtg atgggcgcga cctttggtat cgccttaacg ctggtgatta tcgccggttc
    26521 tgaactgttc accggacaca ccatgttcct cacctttggg gtgaaagcgg gcaccatcag
    26581 ccacgggcaa atgtgggcaa tcctgccgca aacctggctg ggtaacctgg tcggttccgt
    26641 cttcgttgcc atgctttata gctggggggg cggtagcctg ctgccggtag ataccagcat
    26701 cgttcactcc gtcgcgctgg ctaaaaccac tgcaccggca atggtactgt tcttcaaagg
    26761 tgcattgtgt aactggctgg tttgtctggc aatctggatg gcgctgcgca ctgaaggggc
    26821 ggcgaaattt atcgcaatct ggtggtgtct gctggcattt atcgcgtccg gctacgagca
    26881 ctccatcgct aacatgacgc tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta
    26941 cacgctggcg ggtattggtc ataacctgct gtgggtgacg ctgggtaata ctttatcagg
    27001 tgccgtattc atgggattgg gttattggta tgctacgccg aaagcgaatc gtccggttgc
    27061 ggacaaattt aatcaaactg aaacggctgc cggttaatta ctaaggggtt tttacgtgga
    27121 tcatttgcct atattttgcc aattacgcga tcgcgactgt ctgattgtcg gcggtggtga
    27181 tgtcgcggaa cgcaaagcaa ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc
    27241 attagcgttt attccacagt tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga
    27301 agggccattt gatgaaagcc ttctcgacac ctgctggctg gcgattgcag cgacggatga
    27361 tgacgcgctt aaccagcgcg tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt
    27421 ggtcgatgcg ccgaaagctg ccagcttcat tatgccgtcg attattgacc gctcaccgct
    27481 gatggtggcg gtttcctctg gcggcacctc tccggttctg gcacgcctgt tgcgcgaaaa
    27541 acttgaatca ctgctgccgt tacatctggg ccaggtagcg aaatacgccg ggcaattacg
    27601 cggtcgagtg aaacaacagt tcgccacgat gggtgagcgt cgccgtttct gggagaaatt
    27661 gtttgttaac gaccgcctgg cgcagtcgct ggcaaacaac gatcagaaag ccattactga
    27721 aacgaccgaa cagttaatca atgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg
    27781 tgcaggtccg ggcgatgccg ggctgctgac actgaaagga ctgcaacaaa ttcagcaggc
    27841 agatgtggtg gtctacgacc gtctggtttc tgacgatatt atgaatctgg tacgccgcga
    27901 tgctgatcgc gttttcgtcg gcaaacgcgc gggataccac tgcgtaccgc aggaagagat
    27961 taaccagatc ctgctgcggg aagcgcaaaa aggcaaacgc gtggtgcgtc tgaaaggtgg
    28021 cgatccgttt atttttggcc gtggtggcga agagctggaa acactgtgca acgcgggtat
    28081 tccgttctcg gtggttccgg gtattaccgc agcttctggt tgctctgcct attcaggtat
    28141 tccgctcacg catcgcgatt atgcccagag cgtacgctta attaccggac acttaaaaac
    28201 cggtggcgag ctggactggg aaaacctggc ggcagaaaaa cagacgctgg tgttttatat
    28261 ggggctgaat caggctgcga ctattcagca aaagctgatt gaacacggta tgcctggcga
    28321 aatgccggta gcaattgtcg aaaacggaac agcagtcacg cagcgcgtga ttgacggtac
    28381 gctcacacaa ctgggcgaac tggcgcagca aatgaacagt ccatcgctaa ttattattgg
    28441 tcgggttgtt ggcctgcgcg ataaactgaa ctggttctcc aaccattaat ttaatcaggg
    28501 ccagagaatt ctggcctttt tcgtttacaa ctctctttat atattcagca gataaaaaca
    28561 taacccctta ataaatatta tctgctaatg ggttatattc accgttacag cgacttacaa
    28621 aattaagtca ttcactcact gacgccgatt aaatatt
//
LOCUS       DAZGBC010000052        28456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGBC010000052 DAZGBC010000000
VERSION     DAZGBC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.052"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..399)
                     /gene="uspD"
                     /locus_tag="ACHK1J_002204"
     CDS             complement(<1..399)
                     /gene="uspD"
                     /locus_tag="ACHK1J_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO7911463.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSAD"
     gene            complement(426..725)
                     /gene="yiiS"
                     /locus_tag="ACHK1J_002205"
     CDS             complement(426..725)
                     /gene="yiiS"
                     /locus_tag="ACHK1J_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO7911464.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(937..1377)
                     /gene="yiiR"
                     /locus_tag="ACHK1J_002206"
     CDS             complement(937..1377)
                     /gene="yiiR"
                     /locus_tag="ACHK1J_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7911465.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            1478..2077
                     /gene="yiiQ"
                     /locus_tag="ACHK1J_002207"
     CDS             1478..2077
                     /gene="yiiQ"
                     /locus_tag="ACHK1J_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO7911466.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            2185..2952
                     /gene="tpiA"
                     /locus_tag="ACHK1J_002208"
     CDS             2185..2952
                     /gene="tpiA"
                     /locus_tag="ACHK1J_002208"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO7911467.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(3007..3762)
                     /gene="cdh"
                     /locus_tag="ACHK1J_002209"
     CDS             complement(3007..3762)
                     /gene="cdh"
                     /locus_tag="ACHK1J_002209"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO7911468.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESVTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(3869..4858)
                     /gene="sbp"
                     /locus_tag="ACHK1J_002210"
     CDS             complement(3869..4858)
                     /gene="sbp"
                     /locus_tag="ACHK1J_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO7911469.1"
                     /translation="MNKWGVGLTFLLATTSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(5177..6139)
                     /gene="pfkA"
                     /locus_tag="ACHK1J_002211"
     CDS             complement(5177..6139)
                     /gene="pfkA"
                     /locus_tag="ACHK1J_002211"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO7911470.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(6320..7222)
                     /gene="fieF"
                     /locus_tag="ACHK1J_002212"
     CDS             complement(6320..7222)
                     /gene="fieF"
                     /locus_tag="ACHK1J_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO7911471.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(7371..7871)
                     /gene="cpxP"
                     /locus_tag="ACHK1J_002213"
     CDS             complement(7371..7871)
                     /gene="cpxP"
                     /locus_tag="ACHK1J_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO7911472.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            8021..8719
                     /gene="cpxR"
                     /locus_tag="ACHK1J_002214"
     CDS             8021..8719
                     /gene="cpxR"
                     /locus_tag="ACHK1J_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO7911473.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8716..10089
                     /gene="cpxA"
                     /locus_tag="ACHK1J_002215"
     CDS             8716..10089
                     /gene="cpxA"
                     /locus_tag="ACHK1J_002215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO7911474.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(10195..10869)
                     /gene="yiiM"
                     /locus_tag="ACHK1J_002216"
     CDS             complement(10195..10869)
                     /gene="yiiM"
                     /locus_tag="ACHK1J_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO7911475.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENISTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(11018..12001)
                     /gene="kdgT"
                     /locus_tag="ACHK1J_002217"
     CDS             complement(11018..12001)
                     /gene="kdgT"
                     /locus_tag="ACHK1J_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO7911476.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(12261..12881)
                     /gene="sodA"
                     /locus_tag="ACHK1J_002218"
     CDS             complement(12261..12881)
                     /gene="sodA"
                     /locus_tag="ACHK1J_002218"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO7911477.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            13166..14200
                     /gene="rhaT"
                     /locus_tag="ACHK1J_002219"
     CDS             13166..14200
                     /gene="rhaT"
                     /locus_tag="ACHK1J_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO7911478.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(14197..15045)
                     /gene="rhaR"
                     /locus_tag="ACHK1J_002220"
     CDS             complement(14197..15045)
                     /gene="rhaR"
                     /locus_tag="ACHK1J_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO7911479.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(15119..15955)
                     /gene="rhaS"
                     /locus_tag="ACHK1J_002221"
     CDS             complement(15119..15955)
                     /gene="rhaS"
                     /locus_tag="ACHK1J_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO7911480.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            16243..17712
                     /gene="rhaB"
                     /locus_tag="ACHK1J_002222"
     CDS             16243..17712
                     /gene="rhaB"
                     /locus_tag="ACHK1J_002222"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO7911481.1"
                     /translation="MTFRNCVAVDLGASSGRVMLTRYERECRSLTLREIHRFNNGLHS
                     QNGYITWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMTQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            17709..18968
                     /gene="rhaA"
                     /locus_tag="ACHK1J_002223"
     CDS             17709..18968
                     /gene="rhaA"
                     /locus_tag="ACHK1J_002223"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO7911482.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            19511..20335
                     /gene="rhaD"
                     /locus_tag="ACHK1J_002224"
     CDS             19511..20335
                     /gene="rhaD"
                     /locus_tag="ACHK1J_002224"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO7911483.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAHEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            20345..20659
                     /gene="rhaM"
                     /locus_tag="ACHK1J_002225"
     CDS             20345..20659
                     /gene="rhaM"
                     /locus_tag="ACHK1J_002225"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO7911484.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(20700..22094)
                     /locus_tag="ACHK1J_002226"
     CDS             complement(20700..22094)
                     /locus_tag="ACHK1J_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO7911485.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKAAQGNTPVNGQTTAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            22572..23018
                     /gene="frvA"
                     /locus_tag="ACHK1J_002227"
     CDS             22572..23018
                     /gene="frvA"
                     /locus_tag="ACHK1J_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO7911486.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            23029..24480
                     /gene="frvB"
                     /locus_tag="ACHK1J_002228"
     CDS             23029..24480
                     /gene="frvB"
                     /locus_tag="ACHK1J_002228"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO7911487.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            24470..25540
                     /gene="frvX"
                     /locus_tag="ACHK1J_002229"
     CDS             24470..25540
                     /gene="frvX"
                     /locus_tag="ACHK1J_002229"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7911488.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLMLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            25540..27288
                     /gene="frvR"
                     /locus_tag="ACHK1J_002230"
     CDS             25540..27288
                     /gene="frvR"
                     /locus_tag="ACHK1J_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO7911489.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAETI
                     VGLTGYEGFMALLRQG"
     gene            complement(27390..28403)
                     /gene="yiiG"
                     /locus_tag="ACHK1J_002231"
     CDS             complement(27390..28403)
                     /gene="yiiG"
                     /locus_tag="ACHK1J_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO7911490.1"
                     /translation="MALGLTGCDDKKAETETLPPANSQPAAPAPEAKPTEAPVAKAEA
                     KPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFKQGPTGEERT
                     VYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYT
                     QENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGK
                     TFHYYSLAVMISAKQINNLISQDEFDAEAAMKKVSELETLVAQAKEADKGGMNFSFIN
                     SAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYN
                     RLR"
BASE COUNT         7004 a         7363 c         7082 g         7007 t
ORIGIN      
        1 atccgcactc aacttattga tcatcccgcg atatgccggc atcaaacggt tgataaatga
       61 gtggtgatga ccacagacaa ggaggtcgca ctgctctttt tgcataattt ccagcagtgt
      121 ttccggcatt tctccgcgtt caatacgcag ttttgtcttc ggccattgaa tatttttcgt
      181 cagtttatac agcttgttat ccgacttatt cttcaacaat tgaagaatat cttctgttgc
      241 agggaagtag atacccgggt acaactcgct taagccatca tcaatatgaa ttaacgtcag
      301 gtgagcgtca ttatgtctgg cgagctccag ggctttattc accagtaagg catcttcttc
      361 attcccggaa attgccacgc caatgtgttt ataagccatg tttaactcct tctaaagcaa
      421 ctccatcaag ctaacgaacg cagtgataac tcaaaaataa tcatctcggc ctggcatgag
      481 aaggtgaagg ccgcatcaag ctcaacttgc ccctccagat ccttaatctt ataggccacg
      541 ctggcccctt catctgcaga ggatgcagcg gcggcaagct ctttcagttt ggtcataaag
      601 gactctgctt cttcgcgggt ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta
      661 tcaatcaccg taccgatatc aatcgcacat cctttagtac tgcatttatc tacgacatct
      721 ttcatagggc acctctgtat attcgcctct ctgtcgatta cgacttaaga ataatgcgcc
      781 gttaagggaa gaacaaacaa gtaatgatct tatggattac catttttttt gatattgctt
      841 aaggtgaaga gttcagaagg attttccggg cattgtcatt tgcatcgtgg aaataacaat
      901 cccggaaaaa aatagcagta agagatatat taaaggttaa ttacttgatt tattgtcggc
      961 tttatacttc acatcctgag tatctttacc atatttattt tcgccttgtg tgccaacaaa
     1021 cgcaccaaga tcgataagca tcatcaccag aatcaacgtc gggacaaaac gccctaccgc
     1081 ccattgccag acacccggta aaatcgccca gttacccgcc agtagcatcc acgccacaat
     1141 catcagaaat gcccatgcgc cggaacgccc gcgatcatgc aagcgcttaa cggttactgc
     1201 cgctgttggc cagagcaagc acacaaggca aaacgccgcg gtctgaatat cgagtaaatt
     1261 tttacccgcc agtgagaaaa gcaccagcat gcctgcgaac cacaggccta tccaaatcca
     1321 gaaatcacgg cgtccaatac gccctttaaa tgagaataac cattgctgta tggtcatgta
     1381 agttccttga tggttgtctt ttccaggatt ctaccctttt gacaagggcg acagatatcg
     1441 ttttaatcgg agccagtcat aacaaaaggt actgtcaatg aagccagggt gtacgctgtt
     1501 ttttctctta tgttctgcat taaccgttac aacaacggcg catgcacaaa caccagatac
     1561 ggcaacgacc gcgccttatc tgctggctgg agcccctact ttcgatctct ccatcagcca
     1621 gtttcgagaa gactttaaca gccagaatcc cagcctgcca ctgaacgaat ttcgtgccat
     1681 cgacagcagt cccgacaaag ccaatctcac tcgtgctgca agtaaaatta acgagaactt
     1741 gtatgcttct acagcgctgg agcgcggtac cttaaaaatc aaaagcattc aaatgacctg
     1801 gctacccatc caggggccag agcaaaaagc cgcgaaagcg aaagctcagg aatatatggc
     1861 agcggtgatc cgcacactca ccccattaat gaccaaaaca caaagccaga aaaaactgca
     1921 gtcgctacta acggcgggga aaaacaaacg ttattacacc gagacagaag gtgcactgcg
     1981 ttatgttgtc gcggacaacg gcgaaaaggg gctgaccttc gctgttgaac cgattaagct
     2041 ggcgctatct gaatcgcttg aaggtttgaa taaatgacaa aaagcaaagc ctttgtgccg
     2101 atgaatctct atactgtttc acagacctgc tgccctgcgg ggcggccatc ttcctttatt
     2161 cgcttataag cgtggagaat taaaatgcga catcctttag tgatgggtaa ctggaaactg
     2221 aacggcagcc gccacatggt tcacgagctg gtttctaacc tgcgtaaaga gctggcaggt
     2281 gttgctggct gtgcggttgc aatcgcacca ccggaaatgt acatcgatat ggcgaagcgc
     2341 gaagctgaag gcagccacat catgctgggt gcgcaaaacg tggacctgaa cctgtccggc
     2401 gcattcaccg gtgaaacctc tgctgctatg ctgaaagaca tcggcgcaca gtacatcatc
     2461 atcggccact ctgaacgtcg tacttaccac aaagagtctg acgaactgat cgcgaaaaaa
     2521 ttcgcggtgc tgaaagagca gggtctgact ccggttctgt gcatcggtga aaccgaagct
     2581 gaaaacgaag cgggcaaaac tgaagaagtt tgcgcacgtc agatcgacgc ggtactgaaa
     2641 actcagggtg ctgcggcatt cgaaggtgcg gttatcgctt acgaacctgt atgggcaatc
     2701 ggtactggca aatctgcaac tccggctcag gcacaggctg ttcacaaatt catccgtgac
     2761 cacatcgcta aagttgacgc taacatcgct gaacaagtga tcattcagta cggcggctct
     2821 gtaaacgcgt ctaacgctgc agaactgttt gctcagccgg atatcgacgg cgcgctggtt
     2881 ggtggtgctt ctctgaaagc tgacgccttc gcagtaatcg ttaaagctgc agaagcggct
     2941 aaacaggctt aagtctgaca ggtgccggat ttcatatccg gcacttactt tccttaactc
     3001 ttcgccttaa cgcaaaatct cacactgatg atcctgaatt tcctcggctg aagcacggtt
     3061 aagcgtcagt agatttcgtt gtgtcgccag caatacaaat gagttatcac tctgccgtac
     3121 catcgccagc ccgtagcttc ccatatgttc ccgcgcctca ggtacttctt ctgccagcat
     3181 cataaatggg ctgcgttgta ccagttcgct ttccgttacc cgacgcgcca ggtattcatg
     3241 cccgcgcaaa ccacctggca gtggcaacca gcggctgctg atgttcgcca gattgttatc
     3301 cagttgttcg cgcacatcag gacgaataca agagatatga atatgaaaat ggttttgcgt
     3361 acgcccggtg cgggagttga tcgccaaaga aaccgcgcga tcgggaaccg gctggccgta
     3421 ttttttgctc ataaaatcac gcgcctgcca ggccaaccaa aagaagttcg gcgttgaagg
     3481 atcggtcaac aaaggacttt cagtaccgtt aatacgatac gttggcatca acagatattg
     3541 cagtgggcca ttaagatctt ttaaaaccac gtatccggca ttgggtttga cttccgcaca
     3601 tggcgaagga ttttgatttt gctgctgatt gggcaaacat tcctcaagga caatcttacg
     3661 taatgtaacc gactcttcac cggttaattt ccagtaacca ataccggcag ccacaacggc
     3721 gataactatc atcaccaaaa aaagaagacc cgcttttttc atcttttttt ccctgtacct
     3781 caaagagatg caagggtaac gcaaaatcgt gacaaataaa aaacccggtc gcgagaaacc
     3841 accgggtttg ataattatcc tggagaaatc agcgtttgct gatctgatcg aacgtaccgc
     3901 cgttagcaaa atgctctttt tgcgctttcg tccagccgcc gaactcttca tcaatggtga
     3961 ataacttcag ctttggaaac gcattttcgt actttttcgc cacctcagcg tcgcgcggac
     4021 ggtagtagtt tttcgcggca atttcctgac cttctggcga gtagagatat ttcaggtagg
     4081 cttccgccac ctctttagta ccttttttct cgaccacttt atcgaccacc gacacggttg
     4141 gctctgcgag gatagactca ctcggcgtga cgatttcgaa tttatctttc cccagttcat
     4201 tcgctgccag cagagcttcg ttttcccagg caatcagtac atcgccaatt ccgcgctcga
     4261 caaaagtgtt agtggagccg cgcgcgccag aatccagaac ttcgacgttt ttatacagtg
     4321 cccgaacaaa atcctgtgct tttgcctgat cgttgttgtt gtgatgcagc gcgtagcccc
     4381 aggcagccag gtaattccag cgcgcgccac cagagctttt cggattaggc gtgatcactg
     4441 aaacacccgg tttaatcaga tcgttccagt catggatctg cttcggattt cccttacgta
     4501 ccaggaaaac aatggtggaa gtgtacggtg cggagttatc cggcagacgt ttgatccact
     4561 ctttatcaat ccgcccgcgt tccgcaattg cgtccacgtc ataggccaga gccagcgtga
     4621 caacatcagc ttcaataccg ttgattaccg acgtcgcttg tttacctgaa ccaccgtgtg
     4681 actgacgaat caccacgtta tcaccagttt gctgtttcca gtgggcgctg aatgccttgt
     4741 tgtactgttc gtacaattcg cgcgttggat catatgaaac gttaagaagc tgaatatcct
     4801 ttgccataac gctggttgtc gccagcaaaa atgttaaccc tacgccccac ttgttcatcg
     4861 cccgactctc ttatgttgtg ttgtgatgag caaagcgtgc cagaaggtta accaaacatt
     4921 aaagaataaa aaaagattgg ctataacttg cgggtatatg ttgggggtta aaaaggcgga
     4981 agaaatccgc ctcatattgc tgacaaagtg cgctttatcg atgccggatg cggcgcgaac
     5041 gccttatccg gcctacaaaa gtttgtaaat tcaataaatt gtagaattca cgtaggcctg
     5101 ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg tttcaggcta aaggaatctg
     5161 cctttttccg aaatcattaa tacagttttt tcgcgcagtc cagccagtcg cctttgaacg
     5221 gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg aaccagctgt tcgttctgga
     5281 tacctacgca acgaccgccg taacctgcca gcagcagatc gatagcgtaa gcgcccatac
     5341 gggaagccag aatacggtcg taaggcaccg gagaaccacc gcgctggatg tggcccagca
     5401 cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa atgcgccagt tcgtcaacat
     5461 cacacatatg ttcggtaatc gccacgatcg cgtgtttttt acctttcgcg atacccgctt
     5521 tgatttcgtt taccaggtct tcacggctga attcaacttc cggaaccaca acgaattcac
     5581 agccaccagc aatggccgca gccagcgtca gatcgccaca ataacggccc atcacttcca
     5641 ccacggaaat acgctggtga gaagaagagg tgtcacgcag acggtcgatc gcttctacaa
     5701 cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt gcctttgatg tcgttgtcga
     5761 tagtgccagg cagaccgatg cacgggaagc ccatttcggt cagacgcatt gcacccatgt
     5821 aggaaccgtc accgccgata accaccagcg catcgatccc acgttttttc aggttttcga
     5881 tagccacggc gcggatgttc tcgtcgcgga attccgggaa acgcgcagaa ccgaggaacg
     5941 taccgccacg gttgatcatg tcagaaacgc tgtaacggtc tagctgtacc atacggtctt
     6001 catacagacc cagatagccg tcataaatgc ccattacttc cagaccttct gtcagcgcag
     6061 aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc atcaccgccg cttgtcaaca
     6121 caccgatttt cttaatcatg actacctctg aactttggaa tgcaaaatga aatctgttgc
     6181 cggaagtctt cttacacatc gaagtgatcc aacgaatgtg caaatagtat aacaatcact
     6241 tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt ttatacacaa aatgcgggtc
     6301 tggctctctt ttatactgat tatgaaagca tagaccgttt accctccctg ggtacgacgg
     6361 aacaggggtc ctgatggata atcacatccg atcccggaaa acgccgtaaa atagcctgct
     6421 ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag agagtcttcc atttccaaat
     6481 gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg aagatcgtga gcgccgctaa
     6541 cacccggcca ggaagtcacg atatcaataa tttcttgccg ttcctcatca ggcaatgcgc
     6601 gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa cgcgctatat aaaatataga
     6661 tgccgattcc caatgcaaac agagcatcgg cgcgatgcca gccgtaccag gacaacccca
     6721 gcgcgagcag aattgcgccg ttcatcataa catcagactg gtaatgtagc atatcagccc
     6781 gcaccgcctg gctttgcgtc cggcgcacca cccaacgctg aaacgagaca aggataatcg
     6841 tacaaattag cgccacaatt gtcacgataa ccccgacgcc tggatctgtc atcggtgttg
     6901 gagatatcag atgttgaata cccgtcaaaa acaggaatag tgccgaaccg gagataaaca
     6961 tactttgcgc cagcgccgcg agggactcag ctttaccgtg accaaacgag tgattatcgt
     7021 cggcaggttg cagggaatat cgcaccacca gtaaattcgt caacgacgcg ccgatatcca
     7081 ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc ggtataccac catgcaaaaa
     7141 ttttaatcag caatagcagc gaagccatcg ccgtcgcagc aatcgccgcc cgactgacca
     7201 gccgtccata agattgattc ataaatactc ccgctatcaa ctgacgctag tataacggaa
     7261 gcaaatcatc tgcaatgcat taagcagcag gcaaattgag gataaaaaaa acccccacat
     7321 catgtggggg aagacaggga tggtgtctat ggcaaggaaa acagggttta ctactgggaa
     7381 cgtgagttgc tactactcaa tagcttcaac gatgaacttt tttgccattg cgtcacgtcg
     7441 cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa ccgcttgctg ctccggcgtt
     7501 aacaggcgat acatttggtt gcggactttt gccatctcaa cctgacgagc aatttgctca
     7561 ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat caaaattttc tgcggtgaca
     7621 aggcgatgca ttgtctccag ttcgctaaca ttaacaggag gctgttcgtg ccgggcctgt
     7681 tgcataagat ctcgcatctg ctgacgctga tgttcggtta aacttatgcc gtcgaacata
     7741 tggctctgcg tactgcgctg cgtaagttct tcacccggat gccagttatc gcctgaaccg
     7801 acttcagcag cgtggcttaa tgaactgact gccagcgttg aggccatgac ggcagcggta
     7861 actatgcgca tcatttgctc ccaaaatctt tctgtcgcga ttcaacgata gagagtttac
     7921 gattcaggct gcaaacatgc gtcagggggt gtaaaacaac gtaaagtcat ggattagcga
     7981 cgtctgatga cgtaatttct gcctcggagg tatttaaaca atgaataaaa tcctgttagt
     8041 tgatgatgac cgagagctga cttccctatt aaaggagctg ctcgagatgg aaggcttcaa
     8101 cgtgattgtt gcccacgatg gggaacaggc gcttgatctt ctggacgaca gcattgattt
     8161 acttttgctt gacgtaatga tgccgaagaa aaatggtatc gacacattaa aagcacttcg
     8221 ccagacacac cagacgcctg tcattatgtt gacggcgcgc ggcagcgaac ttgatcgcgt
     8281 tctcggcctt gagctgggcg cagatgacta tctcccgaaa ccgtttaatg atcgtgagct
     8341 ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg agcgagcaac agcaaaacaa
     8401 cgacaacggt tcaccgacac tggaagttga tgccttagtg ctgaatccag gccgtcagga
     8461 agccagcttc gacgggcaaa cgctggagtt aaccggtact gagtttaccc tgctctattt
     8521 gctggcacag catctgggtc aggtggtttc ccgtgaacat ttaagccagg aagtgctggg
     8581 caaacgcctg acgcctttcg accgcgctat cgatatgcac atttccaacc tgcgtcgtaa
     8641 actgccggat cgtaaagatg gtcacccgtg gtttaaaacc ttgcgtggtc gcggctacct
     8701 gatggtttct gcttcatgat aggcagctta accgcgcgca tcttcgccat cttctggctg
     8761 acgctggcgc tggtgttgat gttggttttg atgttaccca agctcgattc acgccagatg
     8821 accgagcttc tggatagcga acagcgtcag ggtctgatga ttgagcagca tgttgaagcg
     8881 gagctggcga acgatccgcc caacgattta atgtggtggc ggcgtctgtt ccgggcgatt
     8941 gataagtggg caccgccagg acagcgtttg ttattagtga ccaccgaagg ccgcgtgatc
     9001 ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta ttggtcaggc cgataacgcc
     9061 gatcatccgc agaagaaaaa gtatggccgc gtggaactgg tcggtccgtt ctccgtgcgt
     9121 gatggcgaag ataattacca actttatctg attcgtccgg ccagcagttc tcaatccgat
     9181 ttcattaact tactgtttga ccgcccgcta ttactgctga ttgtcaccat gttggtcagt
     9241 acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac cggcgcgtaa gctgaaaaac
     9301 gctgccgatg aagttgccca gggaaactta cgccagcacc cggaactgga agcggggcca
     9361 caggaattcc ttgccgcagg tgccagtttt aaccagatgg tcaccgcgct ggagcgtatg
     9421 atgacctccc agcagcgtct gctttctgat atctctcacg agctgcgcac gccactgacg
     9481 cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg gtgaaagcaa ggaactggag
     9541 cgtattgaaa ccgaagcgca acgtctggac agcatgatca acgatctgtt ggtgatgtca
     9601 cgtaatcagc aaaaaaacgc gctggtaagc gagacattga aagctaacca gttgtggggc
     9661 gaagtgctgg ataacgcggc gttcgaagcc gagcaaatgg gcaagtcgtt gacagttaac
     9721 ttcccgcctg ggccgtggcc gctgtacggc aatccgaacg ccctggaaag tgcgctggaa
     9781 aacattgttc gtaatgccct gcgttattcc catacgaaga ttgaagtggg ctttgcggta
     9841 gataaagacg gtatcaccat tacggtggac gacgatggtc ctggcgttag cccggaagat
     9901 cgcgaacaga ttttccgtcc gttctatcgg accgatgaag cacgcgatcg tgaatctggc
     9961 ggtacaggtt tggggctggc gattgttgaa accgccattc agcagcatcg tggctgggtg
    10021 aaagcagaag acagcccgct gggcggttta cggctggtga tttggttgcc gctgtataag
    10081 cggagttaaa ctccgcattt gtaggcagga taaggcgttt acgccgcatc cggcatttga
    10141 gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc ctacactccg ggggttattt
    10201 tccccacaac cggcgggaaa aatcttcgat cttgccgctc agtcggcgct tttgcatcgt
    10261 ccgcgtccag cttttcgata acccggcggc ggagagtaaa cggtgatact gatcatcatc
    10321 aaacggcata tgccatgcga tggcggcagc ctcctgcacg gtcacatcgc tgacacggga
    10381 aaccaactcc agcggcgcgt ccgcagacac tttccctggc gctatcacgc tatacaacca
    10441 tcccacttta cccgtgtttt gcatcaactg cgctatatcg ctgatatcaa aatggtaatt
    10501 gagtttatag caaggcgagc gcggctggct gacctgaatt aatgcctctc cccagcggaa
    10561 aatatcgcca atataaacat tactttccgt gagaccgtcg gttgagatat tttcgccaaa
    10621 cgcaggcgca acaaacaact ccgcctgttc cggaaattcc cgcgcccagt agagatagtg
    10681 ctcacgagga taatgacaca gcgctctgtc tggcccgccg tgaacctttt tctccgcctg
    10741 ctcgtcacct tccagcccca gctccgtcag catcaactca ccatcaacct ggattttggc
    10801 aattgcgctg ggtttgcctt cgggataagc ctgaatcttg cctgtgtata catcaaccgg
    10861 atatcgcatc tgttccctct ccacgagaaa tttccccatt agaccatatt tattttatgt
    10921 caggcgtgct gttttcgcgg taagtatcca ggcaggaact caatagcaaa ccggacgaac
    10981 gttagttcat ccggctgtgg ggattaagga agcaggttta tttcaccgta cctaaaattt
    11041 cgattttcgc cgctctggct ttgactttac gtgaccagat agaggtaaga attggcacca
    11101 gaatcgaagt cacaatgact gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt
    11161 taaacgcagg caccatttct gcaatcagca caggtgtcgc taccgcggcc cctgcggaac
    11221 tggaagcggc aattccggca gtgccatcgc caccgccaat caatttatcg gcgataatca
    11281 acggaatacc ggtcacgata attactgcca cacccaacag gatccccagc aaacctgtct
    11341 gggcaattac agtcaaatca atggtattgc ccagtgcgaa ggcaaagaat ggaatcagcg
    11401 tctgcaccgc tttgctgaaa aattctcgta attcagggtc aaggttcccc agggcaaagc
    11461 ccaccaggaa cggtaatact gcgccgacga aaacatgcgg ttcaaacgag gcaatccctg
    11521 cagtgcccag aataatcatc gtcatgagcg gcccggactc caacgacatc aacacaaatg
    11581 ccccagcttc ttcttttgtg ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg
    11641 tcatatccat cgccgccacc agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa
    11701 caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt
    11761 tagttaccac cagcgtaccg gatttacgca gtaccgttcc cgtcgcgctt aattttattg
    11821 acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta atcatcccgt
    11881 tggtgaatga tccaaaatat ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa
    11941 ggaatagcgg gacgagcatc atcccccccg ggattttctc aatcgagcgt tttatctgca
    12001 tttccatcac ctgtcggcat atttttaagg gctgatgcgt aattcctcaa tggagcatca
    12061 gtgttgatgg aaacgaagat agacattcat ttaatcaata aaagtgatca aaaccacatc
    12121 aattgaaaca ctgttttatt tttatcggat cattgttttt attttgaaat tgatcacaaa
    12181 aaaaccaccg ccgttggcga tggttcattt tttaagctga tatgcggcct atacgcctca
    12241 ttgcagcagg cggcaaatga ttattttttc gccgcgaaac gtgccgctgc ttcgtcccag
    12301 ttcaccacgt tccagaactc tttgatgtag tccggacggc ggttctggaa tttcaggtag
    12361 taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc agaaatagct
    12421 tcacccatca gcggagaatc ctggttagca gtagaaacca ccgccagttt atcgcctttc
    12481 agcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttttc aaattctgct
    12541 ttgaagttat ctacggaacc gaaatcacgt tcgatagccg ctttcaggtc accctgcagg
    12601 gtggtgcctt ttttcagacc tttccagaac aggctgtggt tagcgtgacc gccagcgttg
    12661 ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat cagctcttca
    12721 accggcaggt tggcaaattc tggcaggctt tccagcgccg cgttggcgtt gttgacgtag
    12781 gtctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg cggttccagg
    12841 gcatcgtaag catacggcag ggatggcagg gtatagctca tattcatctc cagtattgtc
    12901 gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat taaatgatat
    12961 tgaaaatgat tatcaatgcc gtacttttcg taagggtatg gttttgcagg aaaatgcccg
    13021 agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcgac aacggcggca
    13081 acaggcgaaa ggttaatcga cagcacgatt tttacactca tctcgtcgga gatgtgacgc
    13141 gacgaaaaat gatgaggata agaagatgag taacgcgatt acgatgggga tattttggca
    13201 tttgatcggc gcggccagtg cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg
    13261 gtcatgggaa accatgtggt cagtcggtgg gattgtttcg tggattattc tgccgtgggc
    13321 catcagcgcc ctgttactac cgaatttctg ggcatattac agctcgttta gtctctctac
    13381 gctgctgcct gtttttctgt tcggcgctat gtgggggatc ggtaatatca actacggcct
    13441 gaccatgcgt tatctcggca tgtcgatggg aattggcatc gccattggca ttacgttgat
    13501 tgtcggtacg ctgatgacgc caattatcaa cggcaatttc gatgtgttga ttagcaccga
    13561 aggcggacgc atgacgttgc tcggcgttct ggtggcgctg attggcgtag ggattgtaac
    13621 tcgagccggg cagttgaaag agcgcaagat gggcattaaa gccgaagagt tcaatctgaa
    13681 aaaagggctg gtgctggcgg tgatgtgcgg cattttctct gccgggatgt cctttgcgat
    13741 gaacgccgca aaaccgatgc atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt
    13801 cgctctgcca agctatgttg tcatcatggg cggcggcgcg atcattaacc tcggtttctg
    13861 ttttattcgt ctggcaaaag tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa
    13921 accgctgatc attcacaatg tgttactctc ggcactgggc gggttgatgt ggtatctgca
    13981 attctttttc tatgcctggg gccacgcccg cattccggcg cagtatgact acatcagttg
    14041 gatgctgcat atgagtttct atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga
    14101 gtggaacaat gcaggacgcc gtccggtaac ggtgttgagc ctcggttgtg tggtgattat
    14161 tgtcgccgct aacatcgtcg gcatcggcat ggcgaattaa tctttctgcg aattgagatg
    14221 acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa aatagttact
    14281 atcttcaaag ccacattcgg tcgaaatatc actgattaac aggcggctat gctggagaag
    14341 atattgcgca tgacacactc tgacctgccg cagatattga ttgatggtca ttccagtctg
    14401 ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac aaaatttatc
    14461 cagcgcaaag ggacttttca ggctagccgc caaccgggta atcagcttat ccagcaacgt
    14521 ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca gcaacatcac
    14581 caactgcccg aacagcaact ctgccatttc gttagcaaac ggcacatgct gactactttc
    14641 atgctcaagc tgaccgataa cctgccgcgc ctgtgccatc cccatgctac ctaagcgcca
    14701 gtgtggttgc cctgcgctgg cgctaaatcc cggaatcgcc ccctgccagt caagattcag
    14761 cttcagccgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa cggaagcgta
    14821 ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca cgggtaatgc gataagggcg
    14881 atcgttgagt acatgcagac cattaccgcg ccagacaatc accagctcac aaaaatcatg
    14941 tgtatgttca gcaaagacat cttgcggata acggtcagcc acagcgactg cctgctggtc
    15001 gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg ctacctcggc
    15061 cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt
    15121 attgcagaaa gccatcccgt ccctgacgaa tatcacgcgg tgaccagtta aactctcggc
    15181 gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccgca atgataggcg atgtcagtaa
    15241 cgctggcctc gctgtgacgt agcagatgtc gagccttcat cagtcgcagg cggttcaggt
    15301 aacgctgagg cgtcagtccc gtttgctgct taagctgccg atgtagcgta cgcagcgaaa
    15361 gagaaaattg atccgccacg gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc
    15421 aggccagaag caagttgaga cgtgatgcgc tgttttccag gttctcctgc aaactgcttt
    15481 tacgcagcaa gagcagtaat tgcataaaca agatctcgcg actggcggtc gagggtaaat
    15541 cattttcccc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc tgcaatacgc
    15601 tgtggttaac gcgccagtga gacggatact gcccatccag ctcttgtggc agcaactgat
    15661 tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacatta gtcagacaca
    15721 gattatcggt atgttcatac agatgccgat catggtcgcg cacaaaacag accgtgccac
    15781 cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt tcgacaatca
    15841 caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc cggggttcta
    15901 tcgccacgga cgcgttacca gacggaaaaa aatccacact atgtaatacg gtcatactgg
    15961 cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct taccttaaat
    16021 tttcgacgga aaaccacgta aaaaacgtcg atttttcaag atacagcgtg aattttcagg
    16081 aaatgcggtg agcatcacat caccacaatt cagcaaattg tgaacatcat cacgttcatc
    16141 tttccctggt tgccaatggc ccattttcct gtcagtaacg agaaggtcgc gaattcaggc
    16201 gctttttaga ctggtcgtaa tgaaattcag caggatcaca ttatgacctt tcgcaattgt
    16261 gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc tgacgcgtta cgagcgtgaa
    16321 tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca atgggctgca tagtcagaac
    16381 ggctatatca cctgggatgt ggatagcctt gaaagtgcca ttcgccttgg attaaacaag
    16441 gtgtgcgagg aagggattcg tatcgatagc attgggattg atacctgggg cgtggacttt
    16501 gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg ttgcttatcg cgacagccgc
    16561 accaatggcc tgatgacgca ggcacaacag caactcggca aacgcgatat ttatcagcgt
    16621 agcggcatcc agtttctgcc cttcaatacg ctttatcagt tgcgtgcgct gacggagcaa
    16681 caacctgaac ttattccaca cattgctcac gctctgctga tgccggatta cttcagttat
    16741 cgcctgaccg gcaagatgaa ctgggaatat accaacgcca cgaccacgca actggtcaat
    16801 atcaatagcg acgactggga cgagtcgcta ctggcgtgga gcggggccaa caaagcctgg
    16861 tttggtcgcc cgacgcatcc gggtaatgtc ataggtcact ggatttgccc gcagggtaat
    16921 gagattccag tggtcgccgt tgccagccat gataccgcca gcgcggttat cgcctcgccg
    16981 ttaaacggtt cacgtgctgc ttatctctct tctggcacct ggtcattgat gggcttcgaa
    17041 agccagacgc catttaccaa tgacacggca ctggcagcca acatcaccaa tgaaggcggg
    17101 gcggaaggtc gctatcgggt gctgaaaaat attatgggct tatggctgct tcagcgagtg
    17161 cttcaggagc ggcaaatcaa cgatcttccg gcgcttatct ccgcgacaca ggcacttccg
    17221 gcttgccgct tcattatcaa tcccaatgac gatcgcttta ttaatcctga gacgatgtgc
    17281 agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccaa tcccggaaag tgatgctgaa
    17341 ctggcgcgct gtattttcga cagtctggca ttgctgtatg ccgatgtgtt gcatgagctg
    17401 gcgcagctac gcggtgaaga tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac
    17461 acgctgctca accagctatg tgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt
    17521 gaagcctcga cgctcggcaa tatcggcatc cagttaatga cgctggatga actcaacaat
    17581 gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc tgaccacctt tacccctaat
    17641 cctgacagtg aaattgccca ctatgtggcg cagcttcact ctacacgaca gacaaaggag
    17701 ctttgcgcat gaccactcaa ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg
    17761 cggtggggat tgatgtcgag gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc
    17821 actgctggca gggcgatgat gtttccggtt ttgaaaaccc ggaaggttcg ctgaccggtg
    17881 ggattcaggc tactggtaat tatccgggca aagcgcgtaa tgccagtgag ctacgagccg
    17941 atctggaaca ggctatgcgg ctgattccgg ggccgaaacg gcttaattta catgccatct
    18001 atctggaatc agacacgcca gtctcgcgcg atcagatcaa accagagcac ttcaaaaact
    18061 gggttgaatg ggcgaaagcc aatcagctcg gtctggattt taacccctcc tgtttttcgc
    18121 atccgctaag cgccgatggc tttacgcttt cccatgccga cgacagcatt cgccagttct
    18181 ggattgatca ctgcaaagcc agccgtcgcg tttcggccta ttttggcgag caactcggca
    18241 caccatcggt gatgaacatc tggatcccgg atggtatgaa agatatcacc gttgaccgtc
    18301 tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt gatcagcgag aagctaaacc
    18361 ctgcgcacca tatcgacgcc gttgagagca aattgtttgg tattggtgcg gagagctaca
    18421 cggttggttc caatgagttt tacctgggtt atgccaccag ccgccagacg gcgctgtgcc
    18481 tggacgccgg gcatttccac ccgactgaag tgatttccga caagatttcc gccgccatgc
    18541 tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt tcgctgggac agcgatcacg
    18601 tggtgctgct ggatgatgaa acccaggcga ttgccagtga gattgttcgt cacgatctgt
    18661 ttgaccgggt gcatatcggc ctcgacttct ttgatgcctc tatcaaccgc attgccgcgt
    18721 gggtcattgg tacacgcaat atgaaaaaag ccctgctgcg tgcgttgctg gaacctaccg
    18781 ctgagctgcg caagctggaa gcggcgggcg attacactgc gcgtctggcg ctactggaag
    18841 aacagaaatc gttgccgtgg caggcggtat gggaaatgta ttgtcagcgt cacgatacgc
    18901 cagcgggtag cgaatggctg gagaacgtgc gggcttatga gaaagaaatt ttgagtcgcc
    18961 gcgggtaaac actgccggat gcggcgcgag cgccttatcc ggcctacagg tcggcaacag
    19021 ttgtaggcct gataagacgc gacagcgtcg catcaggcat tgattgccgg atgcggcgcg
    19081 agcgccttat ccggcctaca ggtcggcaac agttgtaggc ctgataagac gcgacagcgt
    19141 cgcatccggc attgattgcc ggatacggca cgagtgcctt atccggccta caggtcggca
    19201 atagttgtag gcctgataag acgcgacagc gtcgcatcag gcattgattg ccggatgcgg
    19261 cacgagcgcc ttatccggcc tacaggtcgg caatagttgt aggcctgata agacgcgaca
    19321 gcgtcgcatc aggcattgat tgccggatgc ggcacaagtg ccttatccgg cctacaggtc
    19381 ggcaatagtt gtaggcctga taagacgcga cagcgtcgca tcaggcattg attgccggat
    19441 gcggcacgag cgccttatcc ggcctacggg tcgtgcatcc gacaacaccg aatttacagg
    19501 aacacagaac atgcaaaata ttacccagtc ctggtttgtc cagggaatga tcaaagccac
    19561 caccgacgcc tggctaaaag gctgggacga gcgcaacggc ggcaacctga cgctgcgcct
    19621 ggatgacgcc gatatcgcgc tttatcacga caacttccac ccacaaccgc gctatattcc
    19681 gctcagccag cccatgcctt tactggcaaa tacgccgttt attgtcaccg gctccggcaa
    19741 attcttccgt aacgtccagc ttgatcctgc agctaactta ggcgtcgtaa aagtcgacag
    19801 cgacggcgcg ggctaccata ttctctgggg gttaaccaac gaagccgttc caacttctga
    19861 acttccggct cactttctct cccactgcga gcgtattaaa gccaccaacg gtaaagatcg
    19921 ggtgatcatg cactgccatg ccaccaacct gatcgccctc acctatgtac tggaaaacga
    19981 caccgcggtc ttcactcgcc aactgtggga aggcagcacc gagtgtctgg tggtattccc
    20041 ggatggcgtt ggcattctgc cgtggatggt gcccggcacg gacgaaatcg gccaggcaac
    20101 tgcccacgag atgcaaaaac attcgctggt gttgtggccc ttccacggtg tgttcggttc
    20161 cggaccgacg ctggatgaaa cattcggttt aatcgacacc gcagaaaaat cagcggaagt
    20221 gttagtgaag gtgtattcga tgggcggcat gaaacagacc atcagccgtg aagagttgat
    20281 agcgctcggc aagcgcttcg gcgttacacc actagccagt gcgctggcgc tgtaaggagc
    20341 aaacatgatc cgcaaagcct ttgtcatgca ggttaacccc gacgcccacg aagagtatca
    20401 gcgtcggcat aaccccatct ggccggaact ggaagcagtg ttgaaatctc acggtgcgca
    20461 taactacgcc atttacctcg acaaagcgcg taatctgctg tttgccatgg tagagattga
    20521 atctgaagaa cggtggaatg cggtagccag cactgaaatc tgccaacgtt ggtggaaata
    20581 tatgaccgac gttatgcccg ctaacccgga taacagcccg gtgagtagcg agctgcaaga
    20641 agtgttttac ctgccttaat gagttgcggt gccggaaaat atccagcacc gcgacttcat
    20701 cagaagaagt atttaaagcg aacgcgtgcg ccgtaacggt catcagtatc atcaccgtgt
    20761 tttttatcgc cgtcgctatc cagaatggag taatacgtcc cgaggtaaat attaaagttc
    20821 tccatatcca tcacattagc gaactgataa gacgcatgaa tggtgtgaat ggtgtacgtc
    20881 ccttcatcat caatccagca gtcgttatca caaactacac cgctgaattc atcaatctta
    20941 ttgtgcgcgt agatataacc gagctcgaaa cgtttccaca gcgcgttaat cccggcagtg
    21001 aaatctttct cattattagc atctaaatag gcggtattaa gattaaccac aatgccattg
    21061 tccggatccg ttttcaggcc gttccaggtc atgctcatac catagccggt acgatcggac
    21121 tgatcgacaa aattaccctt gctatcggta taaccataag cattatttac cacgttcgct
    21181 tccatcgctg cggaaacggt gaattcttcc gtcggcgacc aggcaattac tgggcgcaga
    21241 taagcaacat ttttctgctg ctccatatcg cgtccatgat aattaccgtc attatataaa
    21301 gatgttccgt cttcaagtaa cgtgtttaat tcaaaatacc agttatcgag ttgtttgctg
    21361 acgaggaaat taccgccagc gttagagcgc ccgcggccct ctttcatcat atagatataa
    21421 ccgctgccat cgtcataaag atcgttcgca gtattaccgg aatgttcaac aaaggtatcc
    21481 tgattcagcg ggaacatatc gtaggcttca aaacggccta ctttgacctt ccagtcgttt
    21541 tctttgccaa agaagaatac cgcatcatcg atatttactg aaccgtgcat atcacccagc
    21601 ggttgtgcgg agaacccggc gaaataaccg ttgtccatct ttcgcatacc gtcaaaacct
    21661 aacagaatac gaccattgag attccactgt tcattagtgg gatcgctatt cacatcagcg
    21721 ttggtcattg ccagcaggcc atgattactt tccgcatcca tattaaattc tacgtcgccg
    21781 tagattttta aatcgccata acctgaaagc aaaagattag gtgaggtggc atttttcttt
    21841 gcagattccg ccgttgtctg tccatttacc ggcgtattgc cctgggcggc cttaatttcg
    21901 cggatctcag cggcttgttg tgtctgcagc tgttttacct gcccttctgc ttttgctgcc
    21961 cgcatttctg ccgcttccag gcgttcttcc agcaatgcca tccgttgttc cagcgttaaa
    22021 tgctgtgttt ttgccatacc ggtactacat gccaataata aaccgatggc taaagataat
    22081 gtcgattttt tcattattta cgttccctga aataaaggag gccctacgct ttccaaaagg
    22141 aatacgtata tagagaaata taaagtgtgc actttcgaat ttttaatcca acttcagcag
    22201 gccgatggat agtatcccgc cgcataatat gagtaaatcc gctgatttcc tggaacgtga
    22261 ggaatataac aatagtgccg tttttaacta aatatcgtaa aaaatattag atatgattca
    22321 agctgactca taagccacaa atataaataa cgtttagaca aattatctga gacatttaaa
    22381 cattcacaaa atcattaaat aataactctt tattcacaag atttatttta cctactaata
    22441 tgtgccttac gccgcaaaat ttatccttca ctctgcttta tattgtcacg acttcacgac
    22501 atatccgcgc tcatcacaaa cccgccccgc tattacaatt aactgaaata ttttttcatg
    22561 gagtccgatt aatggcagct cttactgcaa gctgtattga cctgaatatt cagggcaatg
    22621 gcgcttattc cattctgaag cagttagcga caatggcgtt acaaaacggt tttatcaccg
    22681 actcacacca gttcctgcaa accctgctgc tgcgcgaaaa aatgcactcc accggttttg
    22741 gttctggtgt cgccgtgccg cacggtaaaa gcgcctgcgt taaacaaccg ttcgtgttat
    22801 tcgcccgcaa agcgcaggct attgactgga aagccagcga cggcgaagac gtcaattgct
    22861 ggatctgcct cggcgtgccg caaagtggcg aagaggatca ggtcaaaatc atcggcacac
    22921 tgtgtcgcaa aattattcac caggatttta ttcaccaact gcaacagggc gacgccgacc
    22981 aagtgcttgc cttgttaaat caaaccctca gctcataagg aagtggcgat ggagtcatcc
    23041 ttacgtattg tcgcgatcac caactgcccc gccgggatcg ctcacaccta catggtggcg
    23101 gaagccctgg aacagaaagc ccgttctctc ggccatacca taaaagtgga aactcaaggg
    23161 tccagcggcg ttgaaaaccg cttatccagc gaagagattg ccgctgccga ttacgtgatt
    23221 ctcgccaccg ggcgtggcct gagcggtgac gatcgcgcac ggtttgccgg gaaaaaagtt
    23281 tatgagattg ccatctccca ggcgctgaaa aatatcgatc agattttcag tgaattaccg
    23341 acaaattcgc agctttttgc cgcagatagc ggcgtgaagc taggcaaaca ggaagtacaa
    23401 agcggcagcg tgatgagcca cctgatggcg ggcgtctctg cggcgctgcc gtttgtcatc
    23461 ggcggtggta tcctggtggc gcttgccaac atgctggtgc agttcggttt gccgtatacc
    23521 gatatgtcga aaggcgcgcc gtcgtttacc tgggtagttg aatccatcgg ttaccttggc
    23581 ttcaccttta tgattcccat catgggcgct tatattgcct cgtcgattgc cgataaaccg
    23641 gctttcgctc cggcgtttct ggtttgctat ctggcgaacg acaaagcgct gctcggcacc
    23701 cagtcgggtg cgggattcct cggcgcggtg gtgctggggc tggcgattgg ctatttcgtc
    23761 ttctggttcc gtaaagtccg tctcggcaaa gcgctacaac cgctgctcgg ttcaatgctg
    23821 atcccgttcg ttactctgct ggttttcggc gtgctcactt actacgttat cggaccggtg
    23881 atgtccgacc tcatgggcgg gctactgcac ttcctcaata ccattcctcc atcaatgaag
    23941 tttgcggcag cgtttctggt cggtgcgatg ctggcgttcg atatgggcgg ccccatcaat
    24001 aaaaccgcct ggttcttctg cttctcactg ctggaaaaac acatttatga ctggtacgcc
    24061 atcgtcggcg ttgtcgcgct gatgccgccc gtcgcggcgg gtcttgcgac gttcattgcg
    24121 cccaaactgt ttacccgaca ggaaaaagag gctgccagta gtgccattgt ggtcggtgcc
    24181 acagtcgcaa ccgaaccggc tattccttac gctctggcag ctccgttgcc gatgattacc
    24241 gccaatacgc tggcgggcgg tattaccggc gtgctggtga tcgctttcgg aatcaaacgt
    24301 ctggcacctg gtttgggtat ctttgacccg ctgatcggcc tgatgtcgcc ggtgggttcg
    24361 ttttatctgg tgctggcgat tggcctcgcg ctgaacattt cattcattat cgtcctgaaa
    24421 ggattgtggt tacgccgcaa agcgaaagcc gcgcaacagg agcttgtcca tgaacattga
    24481 gttactgcaa cagttgtgcg aagccagcgc cgtcagcggc gatgaacagg aagttcgcga
    24541 cattctgata aacacgctgg aaccttgcgt taatgagatc acctttgatg gtctgggcag
    24601 ctttgttgcc cgtaagggaa ataaaggtcc aaaagttgcc gttgtcgggc atatggatga
    24661 agtcggcttt atggtcaccc acatcgacga gagcggtttt ctgcgcttta ccaccattgg
    24721 cggctggtgg aatcagtcga tgctcaacca ccgggtaaca atacgcacac acaagggagt
    24781 gaaaatccct ggtgtgattg gttccgtagc gcctcatgcg ttaacggaaa agcaaaagca
    24841 acaaccgctg tcatttgatg agatgttcat tgatattggc gcgaacagtc gcgaagaagc
    24901 gaaaaaacgc ggcgtggaaa ttggtaattt tattagcccg gaggccaatt ttgcctgctg
    24961 gggcgaagat aaagtagtcg gcaaggcgct ggataaccgc atcggctgcg cgatgatggc
    25021 ggagctacta cagacagtaa ataacccaga aattacgctg tacggcgtcg gcagcgtgga
    25081 agaagaagtt gggctacgcg gggcacaaac ctcggctgaa cacactaaac cggatgtggt
    25141 gatcgtgctg gataccgccg tcgcgggcga tgttccgggc attgataaca ttaaataccc
    25201 gctgaaactg ggccaggggc cggggctgat gctgtttgac aagcgctact tccccaacca
    25261 gaaactggta gcagcgttaa aaaactgtgc cgcacataac ggtttaccgc tgcaattttc
    25321 caccatgaaa accggagcga cggatggcgg gcgctacaac gtgatgggcg gcgggcgtcc
    25381 ggttgtcgcg ctgtgtctgc caactcgtta tctgcacgcc aacagcggca tgatttcaaa
    25441 agccgattac gatgctctgc tcatgctgat acggggtttt ctgacgacct taaccgcgga
    25501 gaaagtcaac gcgtttagtc agttccgtca ggtggattaa tgctaaacga acgccagtta
    25561 aagattgtcg atctgctgga gcaacagccg cgcacgcctg gcgagctggc gcaacagact
    25621 ggcgtttcag gcaggaccat cctgcgtgat attgactatc tcaacttcac ccttaacggc
    25681 aaagcccgca ttttcgccag tggcagtgcg ggctatcagc tggaaatctt cgagcgccgc
    25741 agtttttttc agttgctgca aaagcacgat aacgacgatc ggctgctggc gctgttatta
    25801 ctgaatactt tcactccccg tgcgcaactc gcctcggcgc ttaatttgcc agaaacgtgg
    25861 gtagcagagc gtctgccccg cctgaaacag cgttatgaac gtacttgctg cctggccagt
    25921 cgccccggtt tgggccattt cattgatgaa acagaagaga aacgcgttat cttgctggcg
    25981 aacttgctac gcaaagatcc gtttttaata ccgctggcgg gcgtaacacg agataacctt
    26041 cagcatttat ccacggtctg cgacaaccaa caccgctggc cgctgatgca gggtgattat
    26101 ctctccagcc tgattctggc gatttacgcc ctgcgtaatc aactgaccga tgagtggccg
    26161 caatatcccg gtaacgagat aaaacaaatc gttgaacaga gcggcctgtt tcttggtgat
    26221 aatgctgtaa gaaccctgac gggtttgata gagaaacagc atcagcaagc gcaggtaatt
    26281 tctgccgatc atgtgctggg gttgctgcaa agggtgccag gcatcgcgtc attgaatatt
    26341 attgatacgc agttggttga gaatattacc gggcatttat tacgttgcct tgccgcaccg
    26401 gtgtggattg ccgagcaccg ccaaagcagc atgaataacc tgaaagccgc ctggcctgcg
    26461 gcctttgata tgagtctgca ctttattacg ctactgcgag aacagctcga tattcctctt
    26521 ttcgacagcg atctgatcgg tttgtatttt gcctgtgcgc tggaacgaca tcaaaacgaa
    26581 cgccagccga tcattttgct ctcggaccag aacgcgattg ccaccattaa tcagctcgcc
    26641 attgagcgcg atgttttaca ttgtcgggtg attattgccc gtagcttaag cgagcttgtt
    26701 gccattcgcg aagagattga gccgttattg atcattaaca acagccatta tttactggat
    26761 gacgcggtaa ataattacat caccgtaaaa aatatcatta cggctgccgg tatcgaacaa
    26821 ataaaacatt ttctggcgac ggcatttatt cgccagcaac cggagcgttt tttctctgcc
    26881 cctggaagtt ttcattattc gaatgtacgc ggtgaaagct ggcaacatat tacccggcaa
    26941 atttgtgcgc aattagtcgc acaacaccat attaccgccg atgaagcgca acggatcatc
    27001 gcccgcgaag gcgaaggtga aaacctgatt gttaatcgcc tcgccatccc acattgctgg
    27061 agcgaacagg agcgacgttt tcgtggattt tttattaccc tcgcccaacc agttgaggtg
    27121 aataacgaag tcattaacca tgtcttgatc gcctgcgccg ccgccgatgc gcgtcatgag
    27181 ctaaaaatat ttagctatct ggcaagcgta ttgtgtcagc atccggcgga gactattgtc
    27241 ggactaacgg ggtatgaagg gtttatggcg ttacttcgcc aggggtgaat gatgtcggat
    27301 gcaccgctaa cacggcttaa cgacgtttag gtcggataag gcgtttacgc cgcatccgac
    27361 cttgtatttc tcagcaaaaa caaaaacgtt taacgcaggc gattataatc atcgaccatt
    27421 tcgttgtact cgcgtaatgc tctcgggaaa gagtcttcga ccatccagct tgagtttgcg
    27481 tcctgaagtt gctctttatc ccagtcagag tacgggactt tatctctgat gcggcgaacg
    27541 tattttttag cctcgagttg atactggcct gccgaattaa taaacgagaa attcatgccg
    27601 cctttatccg cttctttggc ctgcgccacc agcgtttcca gttcagacac tttcttcatt
    27661 gccgcttctg catcaaactc atcttgcgat atcaggttat taatttgttt cgctgaaatc
    27721 atgactgcca gagagtagta gtggaatgtt ttcccttctc tttcttcaat atttttcagc
    27781 tccgcaagct ggcgcttatc attaatttcc tgaatcgccg catgataaga ttcggctaca
    27841 ggttcaaagg cttccagatt ttttaagaat gtctggtgca gcgttttccc tttcgcaaac
    27901 gcatcgtctt tataattttc ctgggtgtaa tatttatcca tttcgttaat ggtattaccc
    27961 aacgccacgg cagcatcgat ataactcacc gctacgccat caataggttg cagcgcaggc
    28021 gttaacgcca cagcactttt tacgcctttt tcacactcag cgaggttgga ttcactaatg
    28081 ccgtagatgc cataaacagt acgctcttca ccggtaggcc cctgtttaaa atctttcagc
    28141 cagtcagcat aacgcgccag actgcgctgt accgggatct gtaacttgtt gtagcacttg
    28201 atatagacat ccattttttc gtcgaaaacc gcttgttcat cgaccaccgg ttgggcaggc
    28261 gtttcaggtt tagcttctgc ttttgcaacg ggagcttcag taggtttcgc ttcaggagct
    28321 ggtgcggcag gttgactatt ggcaggcggg agcgtttctg tttcggcttt tttatcatca
    28381 caacccgtca gaccgagggc catgatggcg actataatag cggaagataa taaattcctt
    28441 ttcatttaat tgcctt
//
LOCUS       DAZGBC010000053        27444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGBC010000053 DAZGBC010000000
VERSION     DAZGBC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.053"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(61..2055)
                     /gene="cirA"
                     /locus_tag="ACHK1J_002232"
     CDS             complement(61..2055)
                     /gene="cirA"
                     /locus_tag="ACHK1J_002232"
                     /inference="COORDINATES: protein motif:HMM:NF007478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO7911491.1"
                     /translation="MFRFNPFIRHLLSTSIGASLFSFPVFTSAHSTLPDAAEDTLVVT
                     ASAVEQNLKDAPASISVISQDDLSRKPVHNLVDVLRDVPGVQITTDDNRKLVNIRGLG
                     GGYTLILVDGKRVSSRTAMFRNNDFDLSWIPVSTIERVEVVRGPMSSLYGADAMGGVI
                     NIITKKTGKEWSGVISGDMTLQEHRDRGDTYGGQFSAGGPLVENLLGIKVYGALSGRD
                     KDTGKQKSADGQETTRAEGFTTRNGGVDFTLTPLENHEFSTGYSFDRQDRESEGSSDK
                     NRMERQNYSVGHNGQWGLGNSELRFYSEKVNNLNTGNTHPITSWNEVIDGKYVFPFTD
                     YNHIVILGGDYRREKISDPVHLTGEKGSENSTSQYSLFAEDEWRLFAPLALTTGVRMD
                     KHENYGTNWSPRAYLVWNATDSVTVKGGWARAFKAPTLIQQSEAWLTNSCKGGCQIIG
                     SPDLKPETSESIEGGVYYSGQEGLLEGVEASVTVYRNEIDDMIANKRSKKLKEAMTLP
                     NFVGITDDGRPVFRYFNINKARIQGLESELKIPLGEQVKLSLTYTYTDGRDLSSGEDK
                     PLNDSPFHNANGRIDWTPVDDWSFYVSANYHGETRAQSSGTETQGGYTLWDAGASWKA
                     TKNITVRSGILNVMDKDLERNDYSYTVDGRRYFLSMDYRF"
     gene            complement(2746..2949)
                     /locus_tag="ACHK1J_002233"
     CDS             complement(2746..2949)
                     /locus_tag="ACHK1J_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911492.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            complement(3103..3660)
                     /locus_tag="ACHK1J_002234"
     CDS             complement(3103..3660)
                     /locus_tag="ACHK1J_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO7911493.1"
                     /translation="MTEQKRPVLTLKRRTDVETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELWAILAE"
     gene            complement(3696..4067)
                     /locus_tag="ACHK1J_002235"
     CDS             complement(3696..4067)
                     /locus_tag="ACHK1J_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HFO7911494.1"
                     /translation="MMKKILLVVAITGMFVQGVWAKSDQQIRDEAKNNVITVSQVSGM
                     TEGTGVTLNGQIVKQLAAEEDHFELSDASGSIVIDVDDEVWRPLALKAGDKVQVVGEV
                     DISDEGNRKATVDVLTLERLR"
     gene            complement(4146..4892)
                     /gene="traX"
                     /locus_tag="ACHK1J_002236"
     CDS             complement(4146..4892)
                     /gene="traX"
                     /locus_tag="ACHK1J_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFO7911495.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRYEHIRQASVNRLWGWAVVAQF
                     GYFLAGYPWYEGNILFAFAVAAQMLKWCEYHSLVWSVSALTLLVLWFPLSSTSYGAVG
                     IILLMLCHRIYRTTHLSVRSMLALCLFPVVALVNLQDGVTAAVSGVLVTYMVVRMVSG
                     IRWPAARFWPADFFPVFYVCHLAVLGVMAL"
     gene            complement(4912..10182)
                     /gene="traI"
                     /locus_tag="ACHK1J_002237"
     CDS             complement(4912..10182)
                     /gene="traI"
                     /locus_tag="ACHK1J_002237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO7911496.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYMDVLARTVGILPPEAGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GQREVTMTVLSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEVREQTLIVTHLNEDRRVLNSMIHDSREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGVSKDDGLITLQDAEGNTQLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVFEPRADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLSGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLREPQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(10182..12407)
                     /gene="traD"
                     /locus_tag="ACHK1J_002238"
     CDS             complement(10182..12407)
                     /gene="traD"
                     /locus_tag="ACHK1J_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO7911497.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDNILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLMAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKSAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPQQPVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEM
                     EQQLPPGISESGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINVHRERGEDVEPGDD
                     F"
     gene            complement(12463..13194)
                     /locus_tag="ACHK1J_002239"
     CDS             complement(12463..13194)
                     /locus_tag="ACHK1J_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911498.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYSPVIQDNTATTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAKEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            complement(13362..13622)
                     /locus_tag="ACHK1J_002240"
     CDS             complement(13362..13622)
                     /locus_tag="ACHK1J_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911499.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            complement(13682..14413)
                     /gene="traT"
                     /locus_tag="ACHK1J_002241"
     CDS             complement(13682..14413)
                     /gene="traT"
                     /locus_tag="ACHK1J_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO7911500.1"
                     /translation="MKTKKLMMVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(14445..14948)
                     /locus_tag="ACHK1J_002242"
     CDS             complement(14445..14948)
                     /locus_tag="ACHK1J_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO7911501.1"
                     /translation="MIMKNLAHVALITVIQLIACNLADWGGAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMTFIIGTLYELRRVCGCV
                     SVREWMQSQVNENTNEELHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            complement(14964..17807)
                     /gene="traG"
                     /locus_tag="ACHK1J_002243"
     CDS             complement(14964..17807)
                     /gene="traG"
                     /locus_tag="ACHK1J_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO7911502.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLQSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWNYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSAS
                     QIMRQNVTMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVLMRNLPM
                     VQTILVGITIGIFPLLVLAAVFNKLTLSVLKGYVFALMWFQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASS
                     SITPTASAAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRD
                     GSMVMDASGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATRELASRST
                     RGSAGMYGDAHAEWGVKPKILGVGGGAGVRGGGRTSIDWNDEDAHQASSGSRASHDAR
                     HDIDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRR
                     AMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQ
                     DSNIRNDVKQQVDNMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNHHKTEALSQNNK
                     YNEEKSAQEGMPGADSPQELMNRAKEYQDKYKQ"
     gene            complement(17804..19177)
                     /gene="traH"
                     /locus_tag="ACHK1J_002244"
     CDS             complement(17804..19177)
                     /gene="traH"
                     /locus_tag="ACHK1J_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO7911503.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(19164..19556)
                     /gene="trbF"
                     /locus_tag="ACHK1J_002245"
     CDS             complement(19164..19556)
                     /gene="trbF"
                     /locus_tag="ACHK1J_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO7911504.1"
                     /translation="MKEKRNDAEQKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPKLLNFHYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRRDNKLREGGNNASH"
     gene            complement(19537..19823)
                     /gene="trbJ"
                     /locus_tag="ACHK1J_002246"
                     /pseudo
     CDS             complement(19537..19823)
                     /gene="trbJ"
                     /locus_tag="ACHK1J_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
     gene            complement(19813..20357)
                     /gene="trbB"
                     /locus_tag="ACHK1J_002247"
                     /pseudo
     CDS             complement(19813..20357)
                     /gene="trbB"
                     /locus_tag="ACHK1J_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
     gene            complement(20344..20634)
                     /gene="traQ"
                     /locus_tag="ACHK1J_002248"
     CDS             complement(20344..20634)
                     /gene="traQ"
                     /locus_tag="ACHK1J_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO7911505.1"
                     /translation="MISKHRFSLPRLDITGMWVFSLGVWFHIVARLVYRKPWMAFFLA
                     ELIAAILVLFGAYQILDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
     gene            20716..21051
                     /locus_tag="ACHK1J_002249"
     CDS             20716..21051
                     /locus_tag="ACHK1J_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO7911506.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSFPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFVIYDK
                     LAPVISLMK"
     gene            complement(21032..21373)
                     /gene="trbA"
                     /locus_tag="ACHK1J_002250"
     CDS             complement(21032..21373)
                     /gene="trbA"
                     /locus_tag="ACHK1J_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO7911507.1"
                     /translation="MHTGDLCFFTGASLFIVGGVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCVLPDNFLFLCGAVMNQCAVPVVSMAVGTIPVITKFERFRRIFVCIMLSSAWSG
                     VMWFFIISLVK"
     gene            complement(21387..22130)
                     /gene="traF"
                     /locus_tag="ACHK1J_002251"
     CDS             complement(21387..22130)
                     /gene="traF"
                     /locus_tag="ACHK1J_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO7911508.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVLAA
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIARLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(22123..22380)
                     /locus_tag="ACHK1J_002252"
     CDS             complement(22123..22380)
                     /locus_tag="ACHK1J_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO7911509.1"
                     /translation="MKVIFTSNRLVDFLIRLVITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFIILYSGVLLFILYGCFSALADLQKPDERKSDERNEDE"
     gene            complement(22394..24298)
                     /gene="traN"
                     /locus_tag="ACHK1J_002253"
     CDS             complement(22394..24298)
                     /gene="traN"
                     /locus_tag="ACHK1J_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO7911510.1"
                     /translation="MKEKINTFFRSLNVNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWA
                     TSETGKTITESFMNKPKDILSPDAPFIQIGRDVVNRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSAHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLT
                     GSGCTANGNCLPHGNGDRMVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLKGTECSEPGGTKTGVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGACLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(24392..24772)
                     /locus_tag="ACHK1J_002254"
     CDS             complement(24392..24772)
                     /locus_tag="ACHK1J_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911511.1"
                     /translation="MSLPAGYYRIEPDIRALVAAMNVHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMTALLEQRLRQDAESAIPRLTWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(24769..25407)
                     /gene="trbC"
                     /locus_tag="ACHK1J_002255"
     CDS             complement(24769..25407)
                     /gene="trbC"
                     /locus_tag="ACHK1J_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO7911512.1"
                     /translation="MNKKIYILFALTAGLFLSAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHADNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKLQ"
     gene            complement(25636..25968)
                     /locus_tag="ACHK1J_002256"
     CDS             complement(25636..25968)
                     /locus_tag="ACHK1J_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911513.1"
                     /translation="MEKKLSSKYIHIYMSISCVFIRLTGKALKLFPFALVMLFTVLFY
                     FMPENVICDIISEWQRSDVSDRIRVIRLYLGTTFLCTWAAVFLEFLCSDEFLPGKRLN
                     KNEEENNG"
     gene            complement(25995..26154)
                     /locus_tag="ACHK1J_002257"
                     /pseudo
     CDS             complement(25995..26154)
                     /locus_tag="ACHK1J_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
     gene            complement(26155..26601)
                     /locus_tag="ACHK1J_002258"
     CDS             complement(26155..26601)
                     /locus_tag="ACHK1J_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911514.1"
                     /translation="MSEGTRVNDFAYVKQALLAVFHRMNTRPLTRLCEKDDIQRGIAD
                     IQWMLHHHYYPSPGQVGFIIFLLRDEKFRVVREDGRQSFLAVEIQSLIDVLKDIRKYL
                     QFVTRYDCDGCIIRLHASAERKYLWIFLECVIVFILCAVLFFLIIC"
BASE COUNT         6491 a         7751 c         6146 g         7056 t
ORIGIN      
        1 tcttgtttat tttcagaata ttatcttata aaggctcctc ctggagcctt cggtttacta
       61 ttaaaaacga taatccattg acagaaaata tcttctcccg tcaacggtat aactgtaatc
      121 attccgttct aaatctttat ccataacatt taatatacct gaacggacag ttatattttt
      181 cgtagccttc caggaagctc cggcatccca taatgtatac cccccctgcg tttctgtgcc
      241 ggaagattgt gcccgggttt ccccatggta attagctgat acgtaaaaag accagtcatc
      301 cacaggagtc cagtcaattc ttccgtttgc attatgaaac ggactgtcat tcagtggctt
      361 atcttctccg ctactcaaat cccgtccatc tgtataggta taggtgagag acagtttcac
      421 ctgctctccc agcgggattt tcagttctga ttcaagcccc tgtatcctag ctttatttat
      481 attaaagtat ctgaaaacag gccgtccatc atccgttatc ccgacaaagt taggcagcgt
      541 cattgcctct ttcagtttct tactgcgttt gttcgcaatc atgtcatcaa tttcattacg
      601 atacacagtg acacttgctt ccacaccttc caggagtcct tcctgtccgc tgtaataaac
      661 accaccctca atactttcac ttgtttccgg tttcaggtcg gggctaccga tgatctgaca
      721 tccccctttg catgagttcg tcagccatgc ttcactttgc tgaatcaggg tcggagcttt
      781 aaatgctctg gcccatcctc cttttacagt aacagaatcg gttgcattcc atacaagata
      841 tgctcttgga ctccagttgg ttccataatt ttcgtgctta tccatacgaa cccctgttgt
      901 caaagccagg ggcgcgaaca aacgccattc gtcttctgca aaaagtgaat actgacttgt
      961 cgaattttct gaacctttct ctccggtaag gtggacagga tcacttattt tctccctgcg
     1021 ataatcacca cctaatatca cgatatgatt ataatcagtg aatgggaata catattttcc
     1081 atcaataacc tcattccatg aagttatcgg atgtgtatta cctgtattca gattatttac
     1141 tttttcactg taaaatcgga gttcactgtt ccctaatccc cattgtccat tatggccaac
     1201 agaataattc tgtcgttcca tccggttttt atcactacta ccttcagact cccggtcctg
     1261 acggtcaaaa gaataccctg tgctgaactc gtgattctcc agaggagtaa gcgtaaagtc
     1321 aacgccacca ttacgggttg taaaaccttc agcacgtgta gtttcctgtc catctgccga
     1381 tttttgtttt ccggtatctt tatcccggcc acttaaagct ccgtaaactt taattcctaa
     1441 aaggttttca accaaaggcc cacctgcact gaactgtccc ccataagtat cgccacggtc
     1501 tctgtgttcc tgtaacgtca tatcccccga gatgacacct gaccattcct tacctgtttt
     1561 tttagtaatg atattaatga cgccccccat cgcatccgca ccatacagtg atgacatagg
     1621 gccacgcacg acctctacac gttcaatagt ggaaacaggg atccagctca gatcaaagtc
     1681 attattccgg aacatagctg tgcgggaact gacccgttta ccatccacaa gaatgagggt
     1741 gtatcctccg cccagtcccc ttatatttac gagttttctg ttatcatcgg tggtaatctg
     1801 caccccgggc acatccctta aaacatccac cagattatgg actggcttac gggataaatc
     1861 atcctggcta ataacactga tactggcagg tgcgtctttc agattctgtt caacagcaga
     1921 tgctgtaact accagagtat cttcagcagc gtccggaagt gtcgaatgtg cagaagtgaa
     1981 cacaggaaat gaaaataacg atgcacctat gctggttgaa agaagatgac gaataaaagg
     2041 gttaaatctg aacatttctg attatatcca taatttgtat tcaatgttgc aagatttacc
     2101 cacactgact tccataaata tgatggagtt tgccccatct tatattaaga atattgatat
     2161 acttctttga ttaaaattta tttattgata tttttatgtc aataccattc ttctctactt
     2221 tactatacat actttcctgt tcgcaaggtc atgtattccc gttccccgtt cgtaccctat
     2281 cggcttcccc ctgctacata ctactttgtg atacagatta tatttttcac ccctgagtat
     2341 taaacattcc tgctcttgct gataggataa ctgtgttata tcgcaggcta ccggatgtgg
     2401 cgctgaatat aacggtcata gataagtcat acatgccctg ctcaggggat tagcgataag
     2461 acggaccggg agagtcatct cccggttttt ctttatcctg ccgcgaatac agcccgactg
     2521 tctgtcagat tcaccggata accctgctgt tatgcatgcc actgtcacct cctgcttctt
     2581 gaaattcatc acatttttta tccctgctgg cttacccggg atgtgaaata tgttaactct
     2641 gtgatgtccg tttgaaagac agggcaactc cttcttacac cactataata ataacgatct
     2701 gccatataag cctgatcccc cggcgctccg caacgggggt tatttttatc ggcacttaca
     2761 ccggcatgca gatgctctct tcagggcaaa atcccgccat gcttcacagg gtgccagccg
     2821 ggccgcattc tgccaggcct cagcggcttc cctgaaaaaa ccacgaatct cctgacggac
     2881 ggcatgacgc cccacgcgct gatagcgata aataccgctg tgctcagata atccactgga
     2941 tacagccatt ttttatcccc cctcttgacc cggctcccgg attatgtcag aaacattctg
     3001 aaacatccct gaacggttgg gtgtttttct ccatcaaaac tctggatgtc tgttttcccg
     3061 attttttcgc tgttgccctc tgtcatacag agggcgtttt tattattccg ccaggatcgc
     3121 ccacagctca tccttgatcc tgttctgtct gcgcaccttt tccaggcgcg cctgcgcata
     3181 ctgttcttct tcctgtgtga tatgttctgt cacatagcct tccgtatcat agcggcaggc
     3241 gccggctttc attgcgcaca gataactttc cgaacgggtg atggctttca gcgcccggct
     3301 gagtttttta tgagagagcg ggatatttcg ctgagacacc tcatccagta aaacctcccg
     3361 gataccgcag gcaagaagcc ggggcgtgtt accgtcaaac aatcccggcc accagggttt
     3421 cagagtgttc accgcctcat ccagcgaggg cagcgtcaga taaatggaca gcgcctgtct
     3481 ggcctgtgct ttcttcgccg ccagctctgc ttcccgggca gccttctccg ccagtttctg
     3541 ctttttcacc ttccattttg gtggcgtggt gacattgatg atggttttcc ggctccgggt
     3601 cggtgtttcc acgtcggttc ttcgtttcag tgtcagtact ggccgtttct gctctgtcat
     3661 aactcacctg ccctcaccgg tatccgttgt tcatatcagc gaagacgctc aagagtcagt
     3721 acatccaccg tggctttccg gtttccctca tcagaaatat ccacttctcc cacaacctga
     3781 actttatctc cggctttcag ggccagcggg cgccatactt catcatcgac atcaatgaca
     3841 atacttcctg atgcatcact gagttcaaaa tgatcctctt cggctgccag ttgcttcact
     3901 atctgaccat tcagcgtcac accggtcccc tccgtcatgc cactcacctg agaaacagtg
     3961 atcacattat ttttagcctc atcccggatt tgctgatctg attttgccca taccccctga
     4021 acgaacattc ccgtaatggc taccaccagt aatatttttt tcatcatctg ctcctgtttt
     4081 ttcctgaatg cgttctgatt taaacaaaaa gactttcaca gttcatccgc tttctgcgct
     4141 tcctgctaca gggccatcac tcccagcaca gcgagatgac agacataaaa taccgggaaa
     4201 aagtctgccg gccagaaacg ggcagcaggc caccggatac cggataccat cctgacgacc
     4261 atgtatgtca ctaacacacc actgacagcg gcagtcacgc cgtcctgcag gttaacgaga
     4321 gcaacgaccg gaaaaagaca gagcgccagc atgctccgta cagaaagatg cgtcgtcctg
     4381 tatatcctgt gacagagcat cagtaatatg atcccgacag caccgtaact ggtggaagat
     4441 aacgggaacc agagaaccag aagtgtaagt gcactgactg accataccag cgagtgatat
     4501 tcacaccatt tcagcatctg tgctgccacc gcaaaggcaa acagaatatt gccctcatac
     4561 cacggatatc ccgccagaaa gtacccgaac tgcgccacaa cagcccatcc ccacaggcgg
     4621 tttacggacg cctgacggat atgctcatac cgtgacagat tcagtcccca gaccagcgca
     4681 aacagtggaa acgcgccccg cccggccaga aacatccacg tctgctccag atgcagaatg
     4741 cggtttgcat ggtccagcac catcaacacc agtgccaccg ttttgatgat atcccgctgt
     4801 ccgggtgacc agaccagcaa cgactgcagc caggtaccgg tccgggcagc cagtgaatcg
     4861 ttacgtgtcg tgttcgtgtt atccgttgtc atcagccttt gtcttcctgt atcagtctcc
     4921 gcccagggtt ttctctttct gcaggtcacg gaccatatcc cgctccatct gctgaagtcg
     4981 ctcctgcagc agattttccc gggcaacctc ccggacagcg tcctgcaccc gttgaggctc
     5041 acgcagcaca ctctccggca gcgcagtttc ccgttctgat gacgaagccc tgtcctgctc
     5101 ctgcccggca atagccctga cagcctgctc tgttttacca tccggctggt caggtttttc
     5161 ctctgccatt tttctgacaa tctcatccgc acggcgttcc gtttcacgac ggatggcctc
     5221 ttcagcctgc cgctgtgcca gcacctctgc cgtgaccggc tcaccgtttc cggcccccgg
     5281 ctggacgctg ttgtccggga tatcccccca cacgcgacca ccggtgatgg caccgggatt
     5341 ccacggacga ccttcaccgg cgattctcac caccacaccg ctgtcggggt tatcacgggc
     5401 aatccggaca ccatcctgca tattatccgc cagcaggctc tcgccgttac ggctgccctg
     5461 cagggccaca aactgtgcat cttcactgcc cttcacccgg ccttcaccac tgaatccccg
     5521 cagcccgttt ccgtcatccg tggtcagtgg gttcagccag ataccggcgg acttgccgtt
     5581 acggtcaaaa gccggcaggg ccacatacgg ctgcggatat ttgcgccccg gtgcaataaa
     5641 ccgtgcagga ctgtctcccc cggacagccc tgcctgacgg agaacagcac gaccagctgc
     5701 cacatcccgc agctcccgcg ccgtactgaa cagccgctct gcattcatga cctcccggtc
     5761 agcccttggc tcaaacacat cgtgggccgt tcctttctgt acggctttgc tgatggcatc
     5821 cgtccagccc tgacggttat cggtgtacac ctgcacatgc tgcttcatac gcgacagggc
     5881 cacataggct gactcaaagc cggccatctg tttccggtta ccttccgtgc cttcaagcgc
     5941 gatggcaaag gtttcactcg ccccctgcgc accgtgagcg gtgatggcat aggccaggtc
     6001 aatatgttgc tctgcctgct cctggccggg gcgaatcacc cgggtctgct gtccgtccga
     6061 cagcgtgaca ctgtcaccgg aaaccgctgt cacagtccag acgctgttgg ccacataacc
     6121 gcgctcccgg tcactcttcg tgaagcgcat ccggtcaccg gttcccaccc ggatggtgtc
     6181 cggggtgtac agtgtgacgc cttcagccac tgcctcccgg ggcgaaatca gctgcgtgtt
     6241 accttccgca tcctgcaggg ttatcagccc gtcatcctta ctgacaccgg caatccggtg
     6301 atacacatta tccaccaggg caagggcatc cgggttattc tcccaggtgg agagacgacg
     6361 cagttccccg tcgcgtatat tcgctgtatt caggacaggc accatgacct gctctttccc
     6421 cagctcaccg gccttttccc gtgaatcatg aatcatgctg ttcagtacgc gccggtcctc
     6481 attcaggtgc gtgacaatca gcgtctgctc ccgtacttcc ggtgtcctgc cggtatagtc
     6541 gcggacaatg gcttcataca gtgtcatggg aacatccggg aaagcctcgc ctttcagcat
     6601 cgccttctgc tgcgcttctg ccagtttcgc ttcctggctg tgactgaact ccgtcacgga
     6661 gtgctccggt gcccatgcac cctccagacg tggcacctga gacggtttca cactctcaag
     6721 cccggacagt gccctttcca catcccggtt aatcaggctg tataccgcct cccgcagttc
     6781 cggcgtctga cgcacaatct ctttcatgat ggccacatcg gcagcactgc gcgtctgctg
     6841 gagacggaaa ggctgaccgg gcgcgatggc ctgcagctgg tccgtgtcac cgctggccac
     6901 ggcacgacca ccaccggccg caatcagggc gtatgcccgt gccatgtcgg tattgcccac
     6961 catagagctc tcatcgagca ggaacagcgt gttgctgaaa tccggcgttt ctccgctgcg
     7021 ctgctgcagc tgcgtgtcat gcagaaaaga cgccagtgtc tgcgcatcca cgccggcgct
     7081 gcgcatctcc ccgaccgcac ggtgcgtggg ccccagcccc acgacacggg gacgctcact
     7141 ctccggcagc atcttcacgg ctgacatcac cgcccggaac tgtgtggtct tacccacgcc
     7201 ggcataaccc tgcaccaccg tgaaacggtc ggacgtttcc agtatcatgc gggtggcggc
     7261 acgctgcccc gacgtcagtg tctccatgag ttcgccgggt accctctcca tcagcggcgt
     7321 gaccgcctcc ttaccttcga gaatatggcg aagaatgctc ttttctgcct catacgacgc
     7381 acgggaaacc agcaggcctg tgccatagcc ttttgccaca tccacataca gtaaatcacc
     7441 gcgttttatc tgcgcattga tttcccctcc cagctcagta aaactggttc cttctgcagc
     7501 aaacgacttc gcctctgtca gcaggtccac catgctgaag gccaggtttt tactttccag
     7561 cacaggaagg gccagatgaa tggcctgctg tgccggcgtg tgaagtgcgg acttctgatg
     7621 actgatggca ctttccagca atgtctcacc ggcacgcgcc tttatctgct cagaaaccac
     7681 cgtaaaggac ggatggcggg cgagtttttc cgcagtacgg gtttcatcca gtgaggaata
     7741 cagccggaca tcacgaccgc tgcgggccag accgttcagg gtggcgttgt ccattgccat
     7801 ctgtgtgacg gaggcaaaaa ccttcgcact gtcactgacg gaatgcccgg gcgtttccac
     7861 ccagccattc tccagcttca gtgccgtgaa cggtgaatca gccacaggca gggtggccgg
     7921 ttcagcccgc cccggcacaa caaccgtcat cgcatcttca ctgacggatg ccacctgcag
     7981 gcggtcaccg ccggagacgc ggagtccggg aattttccct gtcaccctca gtcgctcgcc
     8041 gtctgccacc ggcatttttt ccggccggaa cagcgaccag ctgctgtcca gggaggatat
     8101 acgcaccacc tgcgtttcgc cctgcgcatc ccgcagggtc aggctgtgac tctgcgctgt
     8161 cacccggtcg ataacatagc ggtcatgact gcgtgtctcc gggttccact gctccatcac
     8221 cattcccggg cggtacatat cccgcagata acggctccgg ctgtccagcc agaccggtga
     8281 cagcaccgtc atggtcacct ccctctgccc gagtacgccc tgcgttttca gttcactgcg
     8341 aatggcctgt gtcagtatgg cctgttcccg tactccgctg acctgtgcca cgctctcttc
     8401 tccggctttc acgctggccg caaaatctcc ggccagccgg gcatagcgga cattacggtc
     8461 cggttcactg atgatggttg ccggtcgctg ctctcccccc tgccagcgat atatgttcac
     8521 cccggcatcc ttcatggcca tcagcgcact gcctgtaccg gttcgctgcc cgctgtcggt
     8581 tatcaggacc tgtacgttat gacgtgcggc accgtccagc agggttaacg tctctttcag
     8641 ggagagtttt tcgccctggt cgacgataac ggtactgccc ggcgtgaagg ccatgccttc
     8701 cagcagctga cgacgtccgg ttatcagttc accggacagc cgttcatcct gcttcaggtt
     8761 catctgtgag cgacggtcag cggcaataat ctgcacctcc cgcccctgct cccgggccat
     8821 catgaccagt tcagccaccc gctcacgctg cccggctgca ccgccctgcc cggacacaat
     8881 ggccagcgac gggcgatcct gtgccagaac actgaccgca tcgctgtaac cggccgtccg
     8941 gggaacactt ttctccggat gtacagtcac ccggttctgt ttcatgatgt cacgactgag
     9001 tgcccggacg gacagctcat cgagcacatg aatcccggac gtgaacagcc ccttctcacg
     9061 gtcgaggggg ataagctgct cacggctgat ggcctcatcg ataccggcac gcgcccgctc
     9121 aatcacaccg gcttccggcg gcagtatgcc gaccgtcctg gccagcacgt ccatgtacgt
     9181 gaactgcact tttcgttcac ttaatccggc aatggcctgt gtcaccgcct gctgcacatc
     9241 cggcccgtcc tgtgaagcgg gtccgggtgt ctgcgtgcgg gtctccgccc gttggtctgc
     9301 cgcgtcacga tacgcccgga tgtcgaaccc ggtttccttc agcgtctgca tccattcagc
     9361 cattctgact tcaggatcga cgtgctgttt ggattttcgc gtatccagtg ccgccacatc
     9421 ccgggatttc agcgaggcgt cttccccgac ggcctcccgg atagcctgtg agcgtccgga
     9481 aaaggcctcc accggtacgc ccggcatctc ccacataccg tgcttaccca ccacttcagt
     9541 ttcatagccc agcgcctcaa cgcgctgacg taactcactc tgatatattt tcccgaacgc
     9601 aatacggttc gcgagcacgt tctcggaaaa accggttttc cccactttat cactgctcag
     9661 tgtcttccat tcgccgttat gctgcgtgac attagccacc accgcatgcg tgtgtaactg
     9721 tgggtcctga tcgcgactgg tgtcgtggtt aaagagtgcc atcaccagat taccggtcag
     9781 taccgtttct gactgtccgt ccgtcatcac ccgtgtggaa gccagcgcct ccacctgacg
     9841 gacagcaaaa tccacggcct gattatgtgc atcaatcagg cgcttatcgc cacctaacat
     9901 ggccatcatg gagacacttt tgggggcgga gaaggtcagg tcatagccgg gacgatgctt
     9961 attactgcca tcctgcatac ggcttaaatc cgccccgtcc ggcagcttcc cctccagaag
    10021 acgggtaaag acatccttat caacactgcc ctgcagccca agctgttcag cccccttgcc
    10081 cgcccagcgt tcccccatgc tacccagcac atagtaatta tccttgtcgg tgtaatagtt
    10141 tccggcactg cctgccgatt taacaacgct gaaactcaac atcagaaatc atctcccggc
    10201 tcaacatcct ccccgcgctc ccggtgcacg ttgatattca cctcttcacg gcgctgcatc
    10261 tgctgctgga tatccggatt ctgctcccgt tgccatgcct cataaacggc catatccacc
    10321 acttcaccgg attcactgat cccgggtggc agttgctgtt ccatctcttc ttccggtttc
    10381 attttcagct cctgctcgat cccccctgcc ggaacattca cacctgcatc tgacttctta
    10441 tcgttgatgg caggagacac cggctgttgc ggctgttgcg gctgttgcgg ctgttgcggc
    10501 tgttgcggct gttgcggctg ttgcggctgt tgcggctgtt gcggctgttc agcctgagtc
    10561 acgtcttctc cggaaacaac ctccgggaca tccggttcga agaggctggc catctgacga
    10621 ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc aggattgatg
    10681 tcacgcggaa taaactccgg agcaaccttc ggtcgtgcct gatatttcag ggagagtttt
    10741 actgccggat acggtccggg cagggtgaca taacaggtca gatccggcag agactgaatg
    10801 tcggaataac tgaccagcgt ctggcgctcc atatccttac cggtcgatac cccgtcacgt
    10861 accggatcag caccgtagga atactgctcg ctggctttca ggtgctcttt ctcaccaatt
    10921 tcaccggcag cgaactctgc aatcttatgg ctgggggaac ggaaaaaggc acgggtgttc
    10981 atgacgtcaa acagcgaggc tgccgatttc tcaccgtaga tatcttccag ctgggcatag
    11041 gactggatac cgaacacata acagccaccg aacttacggg cttccggcag gatctccacc
    11101 aggtccggca gtttgtgtaa cgtgggtaac tcatcacaga agaaccacac acgacggtta
    11161 cggttctccc ccatcgccat caggccacga atggcgatgg acagccacat ggagatcacc
    11221 ggtttcaggg aagcatgggt gtcggcattc gacgaaataa acagccagcc gtttttctta
    11281 tcttcacgga caccccgcat ccagtcacgg atggtgaagg actcaccatt atgctcaatc
    11341 ccctgcaggt aacggatggc tttcacgtag ttggtcagca cagcacggat ggaaatcgcc
    11401 gttttctcga ttttctcttc caccaggttg gccgccggtg agttacgcag gaaggtacgc
    11461 agtttttcaa ttttgatgga gagtagtgta tccaccagtt tgctgtagct gcggttgggg
    11521 tcattacgca tcaggtacgc cgcttccgca aaaatggtgc gtcctgagcc ctgccagaac
    11581 gggtcttctt ttgtgcccat cgggatcagg gtatttgccg tattatcaaa atccggctgt
    11641 gtcaggcact ccttccacaa atcccaggcg gcacagcggg catccagcgg attcaggata
    11701 ttatcgatgg acgggtcata gtaactttta acaaattccc ctgatcggtc ataaatcacc
    11761 accatatcac cgcgctgacg ggcgtagttg gccagacgac ggataacttc cgacttaccg
    11821 gccccgaccg tgccgtgcag acagaaattc tggatttcag aatcccggat aatcggcaga
    11881 tcgccaatcc ggatatcgga gtccttgccg tcttttttca gcatcctggc aacctctttc
    11941 ggattgtcag tcagctgacg accacctgtg acttcatttt cactctgctg tttaccctga
    12001 cgccccagaa tccaggagac aacaaagaag gtgatcaggc aaataaccag ggccacgaca
    12061 gacgccagaa cgaatgcaga ccatagctgc tcaccgcacc agatcatgta tttatcatgc
    12121 agtacctgag ccgcgttcat ccggaaggtt ttgccgtaat actggatctc atagaccggc
    12181 tgggacctga ttaaatcccg catgccttcc agcgtggtgc accaccagta aatacagccg
    12241 ttcacaaacg tctgccagct tatttttacc cataaaacca gaccaacgag gatccagaaa
    12301 aaaataaaca ggcagtaaag catgatattg gcgatctggc tgaacatgcg gatacgcatg
    12361 gacgcaatct gaccgccctg ggtcatatcc tttgcgttaa aactcatgat gaaactccgg
    12421 taaagtcagg tacagagcag aaaagagcgg tgtggaatac attcacagaa caaaccatgt
    12481 ataccctgta taccggtggt gatagccgcc tgctggtctg ccggaagaaa ctttttccag
    12541 cacgatatca gtcaggcgca atatttcaga ccagtcactc tcgtgtgaat actggatctg
    12601 aacaaaaagg cgtacccgct cacgataatc cagagccgtg cccggttcat aaaagtggcc
    12661 actgtcagga gaaacacggg atgttttatt gatgagatcg tatttatcgg catgagtctg
    12721 tgacgacaga gaccaggtaa tattttcttc ctgctcacca tcaatacggc tactcccggg
    12781 cgtccaggca atttccttcg cactggtcac gatgatctgt ctggagactt ctgccagttt
    12841 tttgggggta cagaataccg gggccagcac acacagggac aacaaattac agcggccctc
    12901 ttccggctta agcggattgc tcccggattt ctcaggaaaa gatatttttg tcagtatttc
    12961 agttaattcc ttatgcctga gagaattaat aaagtacatg acaagcatca tgagaaacaa
    13021 tataacaata agccctccga taaccactaa aaggtcagta tcaatattat tgctgtacag
    13081 ataatctttt gtggtcgctg tattatcctg tatcaccggg gagtattcag ttacaggctt
    13141 ctcagtaaat tgattattct caccaacata ttcagcgata ttgtctgatg tcataatgac
    13201 tttctccgga agcttccggc ttcgccggca acacttcacg gtcgcacaaa gaatttgcac
    13261 aaccgttcag tgttctcata tttatttcgg aaacgccggg aagtgacaaa atttattttg
    13321 ttactttccg gggttggaca acgagcaacg cgaggcgtca gctattttct tctgcgacgt
    13381 ccggtcggat attcactcac aggaagatat tctgttgcgc ttacgccgat ctcatatgca
    13441 gagaacggtt ccagtgctgt caggtatctc tggcaggcta tggccagttc agaatctccg
    13501 gttgcatcag caaaccggta aagttctttt aataactccc gacatacatg ctcaggatac
    13561 ttttcactgt catagcccag cagaaagagt acctgcgaga gacgagattc tgcacggttc
    13621 atgtattcat ccggggcagg aatatcggcc cggtacaatc tgtccgggcc ggatgggtaa
    13681 tttacaggat gttcgcgatg gatttagcca gctggtcttc cagtacaggc ttcgcttctt
    13741 caaatttcag gttaaccttg ttggcattag ataccacacg ggtctggtat ttatgttgat
    13801 ttccggttga tgtgctggtc tgcacttttg caccggaggt tccctggcgg agagcagcaa
    13861 cattatcagt tctgacttta gatgttgtcc gctctgcaat ctgcacatcc gtgatcatgg
    13921 tgtagttgat atcctccacc attgcatcgg cagccatacc aatcaaccct gtggcaatcc
    13981 ctacacccag cgccgcaccg gcagagctgg agttatatcc tgtgatgccg gtgcccaatg
    14041 ctgcaccggc aatggcacct tcataaccgc tgttcagcca ggtctgggac tcccgcaaat
    14101 ccattttgtc tgccttcagg acattggcct ggatccagta gtatgccttg tcaggtgagg
    14161 tcacaacctg gtagcctttg gctttaacgg catcactgat ttttgcctgc aatccactca
    14221 tatccttgtc agatgtgttt ttgatttgca ggaaaacggt gcgttcactg gaaggctcca
    14281 gccagatggt ttcactcatc tgagttttga cttcaagatt acgcttcttg attgctgtgc
    14341 tcatcgcacc acaccctgaa aggaccagag tggaactgac cagggtaacc atcatcaatt
    14401 tttttgtttt catcaaatta cctttattac aagctacagt tttcttatcg cctatcatca
    14461 tagtcctgcc aacaagtccc aaaagtattt cctgctgaat caaatcctcc atgcatagct
    14521 aatcctgttg ccggattagt acgataaaaa tccacataat caaaattcac atcatcaaaa
    14581 tccagatgca attcttcatt ggtgttttca tttacctgag attgcatcca ttctcttaca
    14641 cttacacaac cacaaactcg ccttaattcg tatagtgtac caatgataaa agtcatcagg
    14701 cttaataacg gagagaataa agaagatact ggcatcagta tataccaaac gcctttactg
    14761 aatttaaact catccgacac atgccgtttt tttcgtattt gagaaaacaa ccatataaac
    14821 aatccctgcc atagtacaat aaagcacagg ataaaaactg tctcagcgcc cccccaatca
    14881 gccagattac aggcaatgag ctgtatcaca gttatcagag caacatgagc taaatttttc
    14941 attatcatct cccaaaccca gcattattgc ttatacttat cctgatactc ttttgccctg
    15001 ttcataagtt cctgtgggct gtcagcaccg ggcattcctt cctgagctga tttttcttca
    15061 ttatatttat tattctgaga taatgcctct gttttatggt gattttgcag ttcagaatac
    15121 tgccctctta caatattttc ctcgccgtgg atgctgttct gagtatcccc gatattgccc
    15181 cgatattctg tgaccatatt atcaacctgc tgcttcacat cgttacggat attactgtcc
    15241 tgcgttcttt gctcaataat accctgatgc ccctgatggt ctgcaataat atcctggctg
    15301 ccaccgcccg aaggtacgct ctccattcct ttaccgatat cgccacggga ttcacgccag
    15361 gcgttatcaa caccaggctg tacctgttcc tgaacaaaag accaggccat tgcccgacga
    15421 cgttctgcaa tctccggtga actggtgttt gtcagaatag cttctgcatc ctgcggcgcg
    15481 tgtttcatca catactgtgc aaactgttgt gacaggtctt cactcatctg cccactcatg
    15541 ctttcagtac gggaggccat ttcagcatat tcatggctgc gggtcatatt cgtcgtgtac
    15601 tggtcgtaac tctgtttcgc tgagttcagg gctgcagaaa gctggtccac cctggaatcg
    15661 gcattattgt cagtatggct gccggattca ctgaccttgc gactggtaaa gtaatcgctt
    15721 gcttctttaa agtcttgtgt agctctggca tcaatatcat gacgggcatc atggctggcc
    15781 cgtgaaccac tgctggcctg atgcgcatcc tcatcattcc agtcaatact tgtcctacca
    15841 ccacctctga cacctgcgcc accaccaaca ccaagaattt taggtttaac cccccattca
    15901 gcatgagcat caccatacat ccccgcagaa cctctcgtac ttctggatgc cagttctctt
    15961 gtcgcctgct catcgctgat attatgcgcc ttcgcatagc tttccaccgc actgcgcata
    16021 cggctggcca tcatggagtc ctgcgcgctc atcgtgctgt cagcgccact ggtgactgaa
    16081 tcactgcttc cccggttaga gccaaactgg ctgagtgtgt tccaggcact ggcaatactg
    16141 ctgctgaaac catgcagggc actttccgcc ttattcatcg actcccgggc catttcctgt
    16201 tgcgctgctg caatctgacg cgttgcatta ataccaaccg gcaaacggga cattgcccca
    16261 ctggcgtcca tcaccatact gccgtcacgg gtctgcgtgg cggttgcacc actaccggtc
    16321 tggtgcgtca tctgaccaaa tgacgtggtg ctgttggtgc tccagctgaa gccgttcacg
    16381 ttattcgtct gcatgttgcc gtaggagtaa ttaccgtcaa ccacactgcc tgccgcactg
    16441 gcggttggcg tgatggaaga agacgcaaag tggctgtata cgctggaaaa tccggcacca
    16501 aggcctcgta ccatcatcca tgacagaggg gggatcattg ctgaaagata acctgcggtt
    16561 gatgccaggt cggagtattt cagctgtatc tgagagagtt cagagagcac gaccggagcc
    16621 ccgttctgct tcgcatagaa tgtcatggca ctgttcagga tggcatacag caacggccag
    16681 gtctggaacc acatcagggc aaacacatag cctttcagga cggacagcgt cagtttgttg
    16741 aagaccgcag ccagcaccag gagcgggaag ataccaatag taatgcccac cagaatggtc
    16801 tgtaccatcg gcaggtttcg catcagcaca tgaccaatgg agacatgtgc cagacgctgt
    16861 ttctccatcg atgaggtggt ggccagattc accaggctgg cggtatcacc gttacgggcc
    16921 gcattactgg tgataccctc tttcagggca ttcatggtga cgttctggcg cataatctgg
    16981 ctggcagact ggctggagcc gtagaaataa ctgtaactgt cactcaccag ttgtctgaat
    17041 aacaggtcag ggtccggcct gccgccaaat atctgctgca cataataatt ccaggtcttg
    17101 cctccggtat tcgtatcaaa attcagctta tccttcagtc cgacggatgc gtccttacag
    17161 gacacaaacg gctctgagct ttgcagaaaa ctgtaattat tattcggcac ctgacgtaaa
    17221 gggctgggct gagaaaagat tatggtgtac gggtcagagg aggccatcag gtcttccagc
    17281 gtgtatttat ggttcaggta aatatcaccc agcacgcagt tctggacgta gtcctgaaac
    17341 agattgatga tttccggatt ccgggacagg aaatcggtgc ttttcacaat caggttcgcc
    17401 ccgaacagca tgcctgtctt gctgtaggtg gcgctgtccg gctgagcgaa caccatctca
    17461 tagccggcca ccatcgcatg cccgatacgg gtcgtcagtg aaagtggcat cgccagaccg
    17521 accggcacat tgtccacccg gtgaacctta accaggtcac tgttatcaat aatctgcacc
    17581 gatgtgcgga cattaaccag caggctgata agcacaaaca cggccaccca gcccagcaaa
    17641 tccatcacgt tgtgccgttg tacccacatc acagccactg cgagaacaga taatgtgagc
    17701 gcaatttttt caatggaatc ccacgtccgg gtccccataa aagcggcaat ggcgttcagg
    17761 ttattgcgca accactcacc accggcaatc acatagactt cattcacagc gtgctccctc
    17821 cgaagtgata gttgttctgg taacggctga gcatacgggc ggacagctgc tggcgcatgt
    17881 agctcatctg acggtcaaca accagcagtg catcctgctg gacctttacc tgcccctgga
    17941 aaaccgcaat ctgttgctgt gcctgattca gattctccag cacactgtcc agcacggtct
    18001 gtggatagtt cccggtggca atcatggccc gggcctgctg aagcagttcc tgaatgtact
    18061 ggagcatgat gtcgtagccg atatagtccg tcagctggta aaccacgctg ctggacacac
    18121 cgagcatctg cgggtcaata agatatttaa agacggggac ggtggtgctg gaaatgaagc
    18181 ctttttccct gtcatccagc ggagtgtcac tcacggcttt attctgaata ctggtgagca
    18241 gttttgtaat ctgtgatttc agggctttat cccgggcgat ggtcacattt gcatccgcca
    18301 ccaccttcag acacttgtcc gaatcgttgc agtggtaaat ttttgccgtt cccccctcca
    18361 taatcgcccg gaggatatcg cggtcagtgg tacgcgccgg caggggggta atctcgctgt
    18421 cttcaccaaa aatcagcgta cctgtgagcg tcataatgaa ctctttcagc tctttgttac
    18481 catcaagcat cctgtttttg gacagcgagt cccagatgat atttatgttc ttcagcaccc
    18541 gttccttatc tttgtcactg gccttgtcac gaacagacga tgactgcccc ccaaccgtgc
    18601 agccctgacg ggacgcggcc cagtcggcga aaatattgct ctcacctgca atatcctgac
    18661 agaccttctg ctgtgacacc tgagtctgag gaaataatcc tccgacaatc ccctgtgcca
    18721 cctgacagga gctgaggttc atactgttaa tgtcattcgc catcttctgc aggaagtctt
    18781 tcgcggtttt gatttcgggc accgttgtct gcagggcaag gtcaaaaaag taccccatcg
    18841 cgttactcat aatctgctta acaaaacgct gcagctgctc gccattaata aaactgaatg
    18901 agccgagata ggcatcgatg ccaccacacc ctgcattgat gtcgggcagt gtcatggaga
    18961 taagctggat atttctgacc tgattacggg catacagaga accgccatag gcatacccgg
    19021 cagcctgccc ctgccagact ccggcctggg tggtattggt ggcaaagccc agcttgttga
    19081 agaactggtt catgtcgctg ttcacatccg ctgacgctgc aggtgtgacc gtcagtaaag
    19141 cggcacaaag aaccagaaga ggtttaatgc gaagcattat tgccaccctc ccttaatttg
    19201 ttatcccggc gcatacggcg tttcagccgg aatatcctga tccccccgtt tattaattca
    19261 tttaaagggt tcctgacgta tacgataaga aatccacctg caaacgccca taacgtatac
    19321 ggaatgacat acatcaggat gttccacata tagtggaaat taagtaattt cgggtccgct
    19381 ttccacgaat caatacaggc atcaaaaata ctgtgcataa tcacccataa aaaagttccg
    19441 gcagcaacga acacaaaaaa cagatcaaaa tatatatcag aaaccctgcg ttcataatca
    19501 taatcccgtt tagtttttct gtttttttgt tccgcatcat ttcttttttc tttcattata
    19561 agtttcctct gatatttctg atgattcatt cagaccgttc atgcggctgt aatatatttc
    19621 agatactgaa atccatccca caattgcccc cacaaaaaca caacagaaac agaaaaggta
    19681 agacacagcg aaacgacaat ttctgcaggg ctggcgggat aaaaaagtcc ctgacggata
    19741 aaggggatat tcaggctgac gattattccc gtcgcgacgc cggatattcc ggcaatgagt
    19801 aacaccacct gcttatttcg cacctttttt ccctccgtac atctgcagaa cggtatccat
    19861 ccgcgccata aaaccggcgg catccgttgc ccctgtaaaa gcggtaatgc ctcaagcgtg
    19921 ttgacgttga ccaggaaggt ggtcggtgtg gccaccggga tattcgggaa gaatgttttc
    19981 agtacatccg gtggtgcagg taacgcctgc ggaaaggccg tatcaccctg accatccagg
    20041 gtatagggaa atacggaaaa tccgtactgc agcgccagtt gtttcagtac cgggtcaaac
    20101 tggtgacagt acggacatcg cccttgcatg aacagcacca ctttccagtc agcaagattg
    20161 acctgagttc cgttgctgag gcggaaccag cgggttgctg ctttctcctg tcgcacctgt
    20221 gcgtctgctg ccggctgagt tgtccgtgct gccattccct ccggatgcca gagtcgctcg
    20281 atttccgcac gtgtggatgc ctgtgtacag ctggccagaa aaagcaggac aacggacagc
    20341 gatttaacgg gaaacatgtt catcatcatt atccttccgt tctctcagta acaactgctg
    20401 tctgacttcc agtgcctcac gctcttcctg actgacgcgg gctatccatg tatccagtat
    20461 ctggtatgcg ccaaacagaa cgagaatggc tgcaataagc tctgccagaa agaaagccat
    20521 ccagggtttt ctgtaaacca gacgggcaac aatatggaac cagacaccca gggaaaatac
    20581 ccacatcccc gtaatatcca gtcggggtaa agagaatctg tgtttactta tcatttgaat
    20641 atcctgttgt ggtttacatg aatttattca cgtatataac ctgcttaact ttcgtgaaat
    20701 taatcagtgt gttatatgaa aaagttatct ttcaaagaaa aactggaaat agtgagaaac
    20761 atcataagag agtcattatc aggtaatgcg gcttttgctt tactggtgta ttcattattg
    20821 cattccttcc ccattaaaac ctttgctgat ttatttgtga cgatactgtt atttatagca
    20881 atcatcgctg ttgtgatatg gctatttcta atcatctatg tttatttttg tgaattattc
    20941 agaagtcact ggatagcagt ctggtttatc ctctggtctg tggtaatcaa cctcactatt
    21001 ggatttgtca tttatgacaa attagcacca gtcatttcac taatgaaata ataaaaaacc
    21061 acattactcc tgaccatgct gatgacaaca taatacagac aaatattctt ctgaacctct
    21121 cgaacttcgt gataaccgga atggtaccga ctgccattga aacaacgggt acggcacact
    21181 gattcataac cgcaccacaa agaaacaaga aattatctgg cagcacacat acacttatga
    21241 catttactac aaagaagaca aacgagctca gcagataaac tcctttatta tttggtcctg
    21301 taaagaaata accaacaccc cccacgataa acaggctggc acctgtaaaa aaacacaaat
    21361 cccctgtatg catatatatc ctttggttaa aaattcggtt taaaatcttc agatacgttc
    21421 aggaactgtt ttgccaggtc atcctgcgaa ataaagccgt atgataatgg acgaacactg
    21481 ccctgtttcg ggtctaccag catcatggcc gggaaatatt tcacgccgag gcgctgcgcc
    21541 tgcccctggt cagtccggga atccggcaat agtggattaa tcacgccatc cacggaaacg
    21601 ggaataacac tcagaccata cgtatcccgg aagccattaa tgacctgcgc cagctgcccg
    21661 tcgatggggt cctgcccccg gtaaaaaaac ataatgccgt aatgctctgc cagtctggca
    21721 atcgcctgtc gctgctgcgc ctggtctgct gccagctggt tccgtaccgt gccgttgtaa
    21781 tggctgtact gcaggttata gtccagttca ggatgtgcca gcatggcctt tttggcgctc
    21841 atggtgaaaa gcccggcctg ctgagtccag taattctgca gccggaaata tttcacaaaa
    21901 ttatccacgc cgggatacag aatggcttcg tacagcgccc gctttgttgc cgtctgcagc
    21961 gcggtcagtt tctgcataat atccggctcc tgacggggag ctgctagtac tggtttattt
    22021 tctttttcct tcggatttat tttctcgtta taccactgcc agcctgcatc tttccctgac
    22081 gccggaaaaa taaagcagca gagtaacagc ggcagtaatg ctttattcat cttcattcct
    22141 ttcatcactt tttctttcat caggtttctg caggtcagcc agtgcagaaa aacaaccata
    22201 cagaataaac aggagcacac cggagtaaag gataatgaaa gcagcagcaa gcaccccgcc
    22261 tgccgtggtt tcttttaccg tatcccacga ccagataatt accggggaaa tgacaattgc
    22321 tgtaataaca agccggataa gaaaatcaac aagacgattc gacgtgaata tgactttcat
    22381 tattttcccg accttactgc cccacctgtt tcagctgacc ggcaatctgc tctttcactt
    22441 tttcggtcag cgcaccatta tccgggattt tctggttatt catcaggtct tcgtagaagt
    22501 tagtgaagtc cagctggtcg aacttaatac gctgcagttc atccacggtt attccccggc
    22561 agtcaggact ttttgcgctg ccaaacccga tacgcaactg cccgttacgc ccctgctgct
    22621 ggactatctg cgccagcttc gaatcaaact ggcagtaact gcgctttttc tgcagacaga
    22681 cacccagcac tttccgggag cagaactcgc ccacactcac cgtcagtttg ttgtcctttg
    22741 cctttcccag cgccttttct tcgctgttgc acttcgccaa acccacatcc tgaccccagc
    22801 caccatcctt acagcagtta ctgaagccgg ctgcggcctt acggcagaat ttcgcctcgc
    22861 ccgtgaaagc cctgacatcc acaccattca gggcggcaac atccttacct gcggcggcaa
    22921 gcgcagccag ctgtgatact gcctcaccaa aatcactgct ttttccgctc tgcgccttgt
    22981 cgcattcacc gtcaaggcag aagacatcac caccacagac catcactttt ccggacgttc
    23041 tggactcgca ggagtacgtg gcatactcat gcagacaagc tccctcatcg gaataaaacg
    23101 cgcactgacg tgaggccagc gtacaggccg gattatcgac atatttctga caggtgccgt
    23161 tatctgcgga ctgtgtgaca tatttatccc ggtatgccca gcacgcctgt gtgatgctcc
    23221 aggttttacc ttccatcaca cccgttttcg taccacccgg ttctgaacac tcagtgcctt
    23281 tcagcacgcc atccgcttta ttaaacggac aactctccac ccactccacc cgtggaaccc
    23341 attccttatc acggaccttc atcctgagtt tcagcgtaaa ggtggaagca ccactgacaa
    23401 gcgattcata gaccatcctg tcaccattcc cgtgcgggag gcaattaccg tttgcagtac
    23461 agccactacc ggtcagaacc tgcccctgtg tgacagaaaa cccggagggc acaggtatgg
    23521 tgaaagtccc gctctgtcct gtaaccagtt gcacattaaa ggctgtgttc ataaagtcgt
    23581 aacgggagtt aaggaaatat agccctgcat gagccgacag cgaggcactt tccactgtcc
    23641 cggcaacagg tacggtaacg ctgaacacca gtttcagacc gttcatactg aaccggaact
    23701 gtgagtgcgg tatcgtcacc tcacgatatt catccgtgta tttccagtct cctgtgatcc
    23761 tggctgtcct ggaacaatat tcctccacca tcgtgtcacg ttcacaggta tagtttgtga
    23821 attcactgcg gtttatctgc tgcgcactgc actgctgtcc ggtatttccc acgatactgt
    23881 cagcccggtt caccacatcc ctgccgatct gaataaacgg cgcatccggt gacagaatgt
    23941 ctttcggctt gttcatgaat gactctgtga tggttttgcc ggtttcactg gttgcccatt
    24001 gtgtggtgcc gttatttttc agaccgccat ccccgccggc agtcacccca ccgtaatact
    24061 gcgtctcatc cgggttcgcg ttatacccgg ggatactctc ctgcggatta aaactctgaa
    24121 tactgccaat accctgcccc tggatctgac gggcaaaatc ggaacctgcc cggtaatcac
    24181 tgttgctgtc ggcaaataca gaaaagctca ggaaaaaaac aattgcacaa atattgcgcg
    24241 gtcttccgga gtcattaaca ttcagagacc tgaaaaaggt atttatcttt tctttcataa
    24301 cctgatccat actggagaat gaagtgatgt catctcaact tcagaacggt gttgattttg
    24361 gtgtatggag cttcctggcg taccgggatt actactcaga taacgacttc agtaacaata
    24421 tcagcgtcct gaagtctgca cgcagtgaac gacgacagta ccggtgatac cagcaatgag
    24481 gaccttccgg ctgaagccgg aaacagagct gaaggtcact gttaaaagcc cccttcaccg
    24541 accatcccca tgtcagacgt ggtattgctg attcagcatc ctgccgcagt cgctgctcaa
    24601 gaagggcagt catttttacc ggacaggcaa aggcaatata aggcagcagc tttgtgaccg
    24661 ggaaaccatg tccctgacag gaggcatatg tacggaatcc gtgaacgttc atcgcggcca
    24721 ccagtgcacg gatgtccggt tcaatccggt aatacccggc aggtaaactc attgcagctt
    24781 atccccggtg ccgtccagca gtccctgagc cacctgccgg cagtctcccg tggcggccac
    24841 tttttccagc gcctgcctga cgcgcagatt tccccggatg atgtcgtgcc cctggctgca
    24901 gaacaccacc agggccggca cgctgcggat gccgtactgt gaaaacaacg tcgggtcgat
    24961 ctgaacacca tcggtcgcgc catcttttac cagggacaac acggcttcag cggtagtctt
    25021 caggtcattg ttcaccatac cccgcagtgt ggccggaatg ccgtagtgcc tggtttctcc
    25081 cagcatccgt ttcagtccct cttcgggaat ggagaacgac acaaaataca cggccccctg
    25141 ctgtggtttg tctttctgag cagcctgctg tttacggacc agttcatcga gaaactggtt
    25201 atcggcatgc tgaaggggat tcgccctgac ctgctgttct gcccatgcct gcagttgtgt
    25261 gtccggcttt tcacgcaact gctgactcag tgtttcctgc tgcttcagga actggcgatt
    25321 ttcgggcgta ttcacatttt ccgaagcatg ggcagaaaga aacaatcctg ctgtcagggc
    25381 gaataaaatg tatatttttt tgttcatata attctcctgc aaacagacag taattaatcg
    25441 tcataatgag gtgctcaata gagcaagcct ggagataact gacgccatgt taaacacaac
    25501 gaagatcgtt gattaacttc tttctccatt tatctttttt ctgagactgg cagcaattag
    25561 ataaacgata ccggatattc actctgttaa atcattaatt catttctgac cgatgcacat
    25621 aaaatgagtc agatattacc cgttattctc ttcttcattt ttgtttaatc tctttcccgg
    25681 cagaaactca tcagagcaga gaaactccaa aaaaacagcc gcccaggtgc ataaaaaagt
    25741 tgttcccaga tatagtctga taactctgat cctgtctgac acatcacttc tctgccattc
    25801 actgataatg tcacagataa cattttcagg cataaaataa aacaacaccg tgaatagcat
    25861 caccagtgca aaaggaaaca attttaatgc tttgcctgtc aaacgaatga acacacagga
    25921 tatactcata taaatatgaa tgtatttact gcttaatttc ttttccattt aaaatattcc
    25981 tctttttgtt taagttacag aaatacacag ttacgtttac gccacatcag gtaaccgaag
    26041 tttttctttg tgtcaggcgg atttttttcc gtctcccggc gcatcacact gcgcccgaac
    26101 ggatggcact gtccgctgtc cgctgtccgg gtacatgttc accaccaggt gtttttaaca
    26161 gataatcaga aaaaacagca cagcacacaa aataaacaca attacacact caaggaaaat
    26221 ccacaggtat ttacgttcag cactggcatg cagcctgata atacaaccat cacagtcata
    26281 ccgtgtcaca aactgcaggt atttccgtat atctttcaga acatcaatta acgactgaat
    26341 ttcaactgca aggaatgact gacgcccatc ttcccgcact acccggaact tctcatcccg
    26401 caataaaaat attataaaac caacctgtcc gggtgatgga taatagtgat gatgtaacat
    26461 ccattgaata tctgcaattc cacgctgaat atcatctttt tcacatagcc tggtgagcgg
    26521 ccgggtattc atccgatgaa acaccgccaa aagggcctgt ttgacataag caaaatcatt
    26581 tactcttgtc ccttcactca tttaatatca cccttaaaca tctgttcatg tatcagtttc
    26641 ggagtgcaaa tggtcataat actgatattc ttatcgctgt tgttaacggt cctgcttcgt
    26701 tttcgcttca taaataacag tctctttttc agagttctga gaagaccatt cccggctggc
    26761 tttattttgt ttttactgtc atccatcata catctgatga tacaaaaaga ttattcacct
    26821 ttcagcggaa acatatactc tgtaattatc aggtcaggga tatctgtaat tacactatgg
    26881 ttttttctgt tctttttctc atcgtccatg ctcagaacgc ctgttacggg tctttatctt
    26941 acccccttta tatccagcct gagaatcgat atacatccac aatatattac cagaccatct
    27001 gtcaaagtat cctcaagaat caacgcagac aagcttgtcg gaaatattga acatttcata
    27061 ttatgctatc cgtcttataa cacgatcaca ttacagtctc acatcctgcg ccacggaatc
    27121 cgaaacagaa tagctaataa cctgaaaaaa aaaggtatac tatataaaga aaaggcaagg
    27181 aagacgccac tctgtgaaac tattgtcctg aatctgaggt atgggggaaa aacccgctac
    27241 agactgttct cctgcaggag gcatcccaat ggattcagag tacatcgcaa tggcggagca
    27301 ttcattattc agacgcagaa taattaatct cattttttca ggagcagata tagcagaata
    27361 aaactgaaca cagaccagca aagcccgact ggatctttag cccgcaaaga aaaagcggca
    27421 atcccggaaa aataacagcc ggat
//
LOCUS       DAZGBC010000054        27371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGBC010000054 DAZGBC010000000
VERSION     DAZGBC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.054"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(92..2962)
                     /gene="xdhD"
                     /locus_tag="ACHK1J_002259"
     CDS             complement(92..2962)
                     /gene="xdhD"
                     /locus_tag="ACHK1J_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO7911515.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2959..3738)
                     /gene="ygfM"
                     /locus_tag="ACHK1J_002260"
     CDS             complement(2959..3738)
                     /gene="ygfM"
                     /locus_tag="ACHK1J_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO7911516.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3789..5117)
                     /gene="ssnA"
                     /locus_tag="ACHK1J_002261"
     CDS             complement(3789..5117)
                     /gene="ssnA"
                     /locus_tag="ACHK1J_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO7911517.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLKAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5120..8218)
                     /gene="ygfK"
                     /locus_tag="ACHK1J_002262"
     CDS             complement(5120..8218)
                     /gene="ygfK"
                     /locus_tag="ACHK1J_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO7911518.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(8540..9118)
                     /gene="mocA"
                     /locus_tag="ACHK1J_002263"
     CDS             complement(8540..9118)
                     /gene="mocA"
                     /locus_tag="ACHK1J_002263"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO7911519.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAFQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            9221..9991
                     /gene="yqeC"
                     /locus_tag="ACHK1J_002264"
     CDS             9221..9991
                     /gene="yqeC"
                     /locus_tag="ACHK1J_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO7911520.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQT
                     RGRRVLITTTTHMFMPKSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            10039..11664
                     /gene="yqeB"
                     /locus_tag="ACHK1J_002265"
     CDS             10039..11664
                     /gene="yqeB"
                     /locus_tag="ACHK1J_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO7911521.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLTTSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(11885..12817)
                     /gene="arcC"
                     /locus_tag="ACHK1J_002266"
     CDS             complement(11885..12817)
                     /gene="arcC"
                     /locus_tag="ACHK1J_002266"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7911522.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(12865..14250)
                     /gene="hyuA"
                     /locus_tag="ACHK1J_002267"
     CDS             complement(12865..14250)
                     /gene="hyuA"
                     /locus_tag="ACHK1J_002267"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO7911523.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(14303..15514)
                     /gene="ygeY"
                     /locus_tag="ACHK1J_002268"
     CDS             complement(14303..15514)
                     /gene="ygeY"
                     /locus_tag="ACHK1J_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO7911524.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(15572..16768)
                     /gene="dpaL"
                     /locus_tag="ACHK1J_002269"
     CDS             complement(15572..16768)
                     /gene="dpaL"
                     /locus_tag="ACHK1J_002269"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO7911525.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVFSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(16826..18013)
                     /gene="ygeW"
                     /locus_tag="ACHK1J_002270"
     CDS             complement(16826..18013)
                     /gene="ygeW"
                     /locus_tag="ACHK1J_002270"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO7911526.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            18492..20270
                     /gene="ygeV"
                     /locus_tag="ACHK1J_002271"
     CDS             18492..20270
                     /gene="ygeV"
                     /locus_tag="ACHK1J_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO7911527.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGIVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(20310..20789)
                     /gene="xdhC"
                     /locus_tag="ACHK1J_002272"
     CDS             complement(20310..20789)
                     /gene="xdhC"
                     /locus_tag="ACHK1J_002272"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO7911528.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(20786..21664)
                     /gene="xdhB"
                     /locus_tag="ACHK1J_002273"
     CDS             complement(20786..21664)
                     /gene="xdhB"
                     /locus_tag="ACHK1J_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO7911529.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(21675..23972)
                     /gene="xdhA"
                     /locus_tag="ACHK1J_002274"
     CDS             complement(21675..23972)
                     /gene="xdhA"
                     /locus_tag="ACHK1J_002274"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO7911530.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVSRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            24388..25143
                     /gene="actS"
                     /locus_tag="ACHK1J_002275"
     CDS             24388..25143
                     /gene="actS"
                     /locus_tag="ACHK1J_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO7911531.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            25222..25295
                     /locus_tag="ACHK1J_002276"
     tRNA            25222..25295
                     /locus_tag="ACHK1J_002276"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25254..25256,aa:Gly,seq:ccc)
     gene            25529..27139
                     /locus_tag="ACHK1J_002277"
     CDS             25529..27139
                     /locus_tag="ACHK1J_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO7911532.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDIISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAINTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            27206..>27371
                     /locus_tag="ACHK1J_002278"
     CDS             27206..>27371
                     /locus_tag="ACHK1J_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO7911533.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMI"
BASE COUNT         6698 a         6674 c         7003 g         6996 t
ORIGIN      
        1 acgccttgag aacggcttat ctcattttcg cagtcactat ttagtcatcg ttcaaacaga
       61 gaaacaggcc gccgaagcgg ccatttatta cttatatttt ttccagcgcg gtgagtattt
      121 tctccggtgt gaaatgccat tcgcgtaacc agatgccgca ggcatcgtga attgcggtag
      181 caatcgccgg agctgcgcca tttacaccaa tttccgagat cgatttcgcc ccgaacgggc
      241 cgactttatc gtcgctcggc accaacacag cgcggaaatc acgcggaatg tcaccaattt
      301 tcggtgcgcc ataactgcgt aaatcacgcg ttaacgggtg accttcggcg tcataaatga
      361 tctcttcgct catactgtgg ccgatagcac gcagggtggc accgtagatt tgtcccaacg
      421 ctaactctgg attgaccggt gtaccgcagt ccagcaaggc gtagaattta tccaggcgga
      481 tttcacccgt acgcgtgttg acggcaactt cagcgaagtt tgcgccatac gggaaagcga
      541 aatcaggcgt gatataactg ccagttccca ccagtgaacc aaagccggtg ccggtttcgc
      601 ctttatgggc aatatccccg aaactaactt cgcctttctt gccgcgcacg acgcccggcg
      661 ttgctagttg aacatctgcc actggctcac ccaacatttg cgcgccgtgg aacagaattt
      721 tctcccgcag attttccgct gccaaacgcg ccgcgttacc cgagaagcaa gtaccggacg
      781 aggcatatgc gcctttatca aacaacgcat gatcggtatc accggagata acatgcacgt
      841 cctgcggtgg gcagtgcagc acttctgctg ccagtttcgt cactacagta tccagaccag
      901 taccaatatc cgcaccgcca gaatgaacga taaaggtgcc gtccgattcc agtttgatca
      961 tgcagttagc ctgatcgata tccgggatac ccgatttctg catgataatg gcgacgccgc
     1021 gaccgatgtg ccagtcacca ttttgcggtt tcggtgaaga ccattggatc atctcgcgac
     1081 cctgacgcag gatctcttcc agtgcgcagc tggcggcgga aggaacggag gttggcgctt
     1141 taccttcacc aattgcaccg agaattttca gctcttgccc ttcgtgtacc cggttacgtt
     1201 cgataatttc cagttggtcg atctgtaact gttcagcaag ttccgctaat gccatggtga
     1261 tagcgaagtt acctttcggt gcgccataac cctgataagc accatttggg caaatgttgc
     1321 tgtagtaggt ggtgacctgg aaatcgacgt tatcgcacgg atataagggc agcgacagcg
     1381 ccggtccgtt acacggtacg gtgagtgagt ggttgccgta agggccagtg ttggcgcgga
     1441 aatccatctt cactgccgtc aggcgaccat cttttttcgc tcccagtttg acggtgactt
     1501 tcgcgacgtg acgagaggtg ttagcaataa actcttcttc acgggtgtag cggaacagta
     1561 ccggacgccc ggtcacgcag gttgcccatg cgcacacttc ttccagcagg atgtcctgtt
     1621 tggaaccaaa accgccgcca actcgctctt taatgacatg aactttatgc tgtttcatgc
     1681 ccacgaggcg cgcgacctgg cggcgtaagt gccatggtac ctgggtggag gcgtggataa
     1741 ccagacgatc gccatccatg cgggtaaagc agatatgtgt ttcagtcggg cactgctgcg
     1801 cctgcgttga gttataggtt cgctcaatga tcacatcggc atcggcaaag cctttgtcca
     1861 tatcgccaat atgaccatga atactggcgg cgatattttt gcgggggcga gaaccgatcg
     1921 ggaagttaat gatcatatgc tcgccgcgct gggctgcatg gctgttatcg tcttccagag
     1981 tatctggcgc accagcaaca tacaccaccg gttcatcgtg cacgacaggc gcatcttccg
     2041 ccattgcttc gtcgatcgac attaccggct taagcacttc atattcaacg tcgataagct
     2101 tcagtgcttc gagcgcaatt tcttcacttt ctgcgaccac cgcagcaaca cgatcgccga
     2161 cgtgacgcat tttcttgccg aacatacggc ggtcaagcgg tgacggttcc ggtgcgctct
     2221 gcccccccgg ggtatagtag atatccgggc aattcagatg agtaataacg tgaacgacgc
     2281 ccggtaaggc ttcagctttg ctgacatcca gatgagtaat cagtgcgtga gcgtgtgggc
     2341 tacgtaacat tttaatgacg caggcgtcag ccgttacgcg gtcttcaaca tagcagggct
     2401 tcgcctgcac catttttgcg gcatcagttt taggataatg cttaccaatg acttcaaggt
     2461 cgtcacggaa agtcggagcg atatcaatgg tggcctgcgg attattttta cgtgcaaccg
     2521 ccagttcaat gacctgatag tattgctgcc agccagcatc gcggctaaac agacctgaga
     2581 gcgcgtcgtc gatctcttcg cgggtaggtg cggcgatgcg atcgagaaga tcggtgataa
     2641 tcagagctgc agctggatcg ttataaccag actgcaccac gccaacatca accattgcct
     2701 gttgaaccag acttaactcg ttccatttgc ccagagattc tgcggtacga atatctgcct
     2761 tctctaactg tgcggcaata agcaaggacg cgttaacgat attaccgtta aagataattg
     2821 cgtcagaacc ggcaaacccg aagccatcat cactgttgcg tacagagtgc attcccatgt
     2881 taaacaacag cttttgcacg ttttcgcctg gattaacggt tagctcctga ggcgcgccat
     2941 ttaaagtaaa gtggatgatc atacggcttc ctccccagcc tgttggcagt cggcatacag
     3001 gtcggctacc agaactcccg tgatatagcg tttataggcc acgctgcccc gcaaatcttc
     3061 ctgcgggaaa atggcgttgg cgacagcctg ttcaagtgca ttgccttcca gattttgttt
     3121 ttcgacatcg tgcagacgca gtgctttact ggcgacgcca tccagcgcaa ttcgcatacc
     3181 gtcgtggtct gtcattgcaa cggctgccgt cacgacggtt aaacctgcct gagaacggct
     3241 aattttccgg gtcgcacagg tgcgatacgg atctttaatg ataatttcgg ttagcaggcg
     3301 atcgcatggg caggccaggt agtcctcgat tgacagcgtt tcgccgttgc caaaaaccag
     3361 ttcagcatcc agtgccagca ggacgggaag cagcaccgac tcttcctggc gggcggcgat
     3421 ttcaccacca atggtcgact gattacgaac atggcgtgag taaacaaaac cgagggcttc
     3481 acgcagcgct gcaggaataa atcgcgcatc acgcagtggc tgcaagcgag acattgcgcc
     3541 aatccgcagt gcaccgttat cccagtcaac ccagtccagt tccagatcct gtaaggagat
     3601 ggcaatcttt ttatcggtac gggttggtgt agcattgagt ttgctgcccc cggcgaacca
     3661 gacggcttca tcctggtagc ggcgcttcag ttccagcgcc tgttcgacag agtcgggcct
     3721 gaaaaattgt tcaatcatct tctttcctta tttaaccctc ttccctgcgg gaagagggca
     3781 tctgtcattt atgccagcgc atccatccga cgccacatac tggcagcggc ttttctggct
     3841 tgtgcataaa tggaatcgca atcgaagtta aactgacggt cttcatagac catcacacca
     3901 ttgaccatca cgctgtgaac gctgcctgag cccataccga aagcgatatg cccggcgata
     3961 ttgtctgcca gcagcggcgt cggcgagttg taatcgcaaa tggttaaatc agctttgtaa
     4021 ccggctttca gaagcccaaa tttcgcgcca aagttgcggc tcatcagttc gttaccgtta
     4081 gtcagggctt tggcaaaact gtcaggccac agcggaccac ccgcatcgcg atgtttaaag
     4141 aaggcaaatt tcatctcttc aaacatgtcc gaaccaatgc cgtccgttcc caacgccaga
     4201 ttgcggatgt cgctaaggtg atggttgtag ccgacatggt tgttcatgtt tgaacgggcg
     4261 ttatgcacca ggaacgcatc gcgctgattg agtagggtga tgtcatcttt cgacaagtac
     4321 agcccatgag cgaccagtgt tttgctgtcg atgagatcga attgcgccag tcgtgccagc
     4381 aggtctttgc cgtaccagtg gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga
     4441 atatgcaaac cacggcctgt ggctttcacg gcttcacgca gcatctccag accggcatct
     4501 ggcacggtaa acggcgcgtg agcaccgata tgtgcttcca ccagatacgg ctcggttgct
     4561 gctttcttcg cctcatcaat ctgacgggca aagcggatgt tttcttctac accttcctgc
     4621 aactctttga tgccgttgtt acggtcagta gtttcaaaac aggtcatcgc gcgcaggcca
     4681 acttttaaaa atgcgtcgcg caatgtggag agcgacccgc cgatatacgc cggagaggca
     4741 tggtgatcga taaccgatgt acatccgctc ttaatcgctt ccagggaaca aatcagtccg
     4801 ctgtaataga gcgactcttc atcaagggcg cgatcgagcc gccaccagag atttttcagc
     4861 gttgagatga aatccgggca aggggcgatg tttgccataa ttccgcggga aagccccgag
     4921 taaaaatggt tgtgcgagca gacaattccc ggcatcacaa tccgaccatg catctctttg
     4981 aagctggcgt cggggtagcg ttgcgtcagg gcatcgccga tagcgacaat cacatcgttt
     5041 tcgatggcga tatcaacgcc ttcctgcact tttgccgggt gtagctgtac cgcagtgaca
     5101 ttcttcagaa tcaacatgat tacacctcca cgcggcccag cagataatga tgatgctggt
     5161 ggacatggct gatgatgcgg cacatatcgt tcagctccgg tggtacgttg ttaaactgac
     5221 ctttgctgtc gatgtttaac acccagcttt ggttattcag acgtactcgt acccggcaat
     5281 cttccacaag gaagcctggg ttgctgctgt tatcaaagtc ttgcgccagg ctgaagacgg
     5341 tgattttgtc tttgtacggt ttaccgttcc acggacagaa ctgagcgcag ttgccgcatt
     5401 cgttacagta agcgtcgagg tgcagcgtct ggaaacggtt ctggaagcct gggaccgcaa
     5461 tggagacgtt ggcgcggttc gggcagacat ccacacactt gctgcaaacg tagttacatt
     5521 cgaggcagcg agcggcttcc tgggcgacaa acgcgtcacg atcgtcactg ttcaccagcg
     5581 tgatagagat atcgccttta cgttgataga tttccgccgg attgacgttg ttccagtatt
     5641 tatcgttctg gtgggaacgg atattttccc gactaaggat ggcatcggtc gcccgacgcg
     5701 cggttccgac agcagcgaca atggaggatg gtccgcgctg tacgtcgccg atcataaaga
     5761 cgtcagtcag acgagtttcg ccattatggt cgacgtctgg ccagccgttt ttgtccagcg
     5821 gcacgcccat cgcattcagg gcttcagtat cctgctgttc accaatggcg gtgatcaggc
     5881 tgtctacatg cagtgttact gtttcgttgg tttcaaccgg acgacgacga cctttctcat
     5941 ccggttcgcc aagcgacata acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg
     6001 gattgttcag gaaacggaac tccacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc
     6061 atgcgggcat ctcttgcagt gaacgacggt aaacgatcgt tgctttttct acgcctggaa
     6121 cgcgtaacgc cgcacgagcg cagtccattg cggtgttacc cgccccgaca acgaccacat
     6181 gtttgcccag cttgagcgct gtacccttgt tgtattcacg caggaagggg agtgatttcc
     6241 agacattttg gttgtcgccc gccagtttca caccgctatt tttatcagtg ccggtggcaa
     6301 tcagaacata gtggaagccc tgatttttta actgttcaac ggttaaatcg ggtgagcagc
     6361 catactcaaa tttcacgccg tgatcggcaa caaaatcgat atcgtgctga attaactctg
     6421 caggaatacg gaactgagga atgatatttt tcactacgcc gcccgcattg gcttcgcgtt
     6481 caaacagcgt aaccggatgg cccgctctgg caaggaagta acctgcagcc agacccgccg
     6541 gacctgcacc aatcacggca accggatggc gtgaaccaga accggctggt ttgtgccagc
     6601 gttgcttata ttcatcccaa cctttttcca gcgcgacttt tttcagttcg cggatattca
     6661 gcgcactgtc gtaatccagg cgggtacagt tgtattggca ctggtgatcg caaatatgac
     6721 cggtaatggc aggtagggcg ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt
     6781 gttcgccaag cagacggatg tattccggaa tatcttgctt gatagcgcag gcagtaacac
     6841 agggggcaac gtagcagtcg gtcagcggca ggtcttctgc cacttcaata cgctcttctg
     6901 gcttccagtg tttctgggtg tattccatgg ttaacgcgtc tgctgccagt ctgttcagtc
     6961 gttcgacgtc aacatggtca agtccccagg cgtcggagcc ttccagctcg cgcatgcagg
     7021 cacttaagcg cagatagccg ccaggtttca gcagatcggt tgccatagta atagggcgaa
     7081 taccagtatc aaaaatatcg cggatagtca gctgactggc accaccggaa taagaaatgg
     7141 gcagtttgcc gtcaaaggcg cgagagagaa ctgctgcaac attgatggag agcgggaaca
     7201 gcgcacggcc tgacatatac atctcttcac cgggcagtgc gcctttattg ttgatggtgc
     7261 cgagagtgtt agtcagtttt acgccaaagc cgagtgattt ttcttttgcc agtgccatca
     7321 ggcgttccag catttccagt gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt
     7381 ttaagcctat gtaaccgaaa ccgcagacat cgaggatctc acgaacacgc gcgtacccga
     7441 gtaaggtcgg gttaagtttg acaaaggtgt tgagcccttt ttcttccagc atgtagcggc
     7501 aaatggcttc gatttcatgc ggaggacagc cgtgcatggt ggagagggtg acgccctgca
     7561 ccatactggt ggggatgcga gcgggtaaag cttgcaagct ttcgcgtttt tcctgcaatc
     7621 cgtggcgggc taaaaatgcg tcatcctgga gtaatttatt cagcgtatcg cgatattgcg
     7681 cgaatttcgg atggtcagat gcgtccatca tattgtcgat gaactgttgc atcggcggtt
     7741 gcttaatacc ttcaaggttg taaccgacgc tcatattaaa gatgaacgat ttaccggaat
     7801 cagaaggctg gaacatcgct tcgagaaggt gcagggcaaa ccaggctttg aggtattcat
     7861 cccaggcttt aagcagggta aattcggtag accattcggt gttaaagcac tcgtcttcgg
     7921 catcgataca aggcttttcc agctccaggc ggtcaagaat ttggacggtt tttagttcga
     7981 tgaagcgtcc gccagtcagc caggaagtga caatattttg cgcgagctgc gtgtgcgggc
     8041 cagcggcagg gccgacggga gtggcacagg tttcaccgaa gacgctaaca gttttacctt
     8101 ttacgggtga gtaaaactgt tgctcgggaa taccaaagat tgagcgttgt tgttggtatt
     8161 catcaaatat gcgcgtcaaa agttcctcaa acggaatggg acgcataata tcccccataa
     8221 ccctctcctt agcttataca gatatcattg agtgaccggt gttgagaaat ccggtggttt
     8281 agggggatag agcaacaatc gcaccactct tgccaggttt ggcgggaaat ataaaaaaat
     8341 gtgaggcggc tcgaaactta agggcttttt gtgaatgaac tcacaggtgc caaatgagaa
     8401 ttaaaatcac tcctgaaaat tattattgcc agcccattta tcactttttc tcttttctca
     8461 aaattagtga ttccttatca ttatgataaa gagtgctgga ggggttaaat atttgaacgc
     8521 aatcgtttaa ctacgttttc taaatttcag tatacctttt cttcgctgta ataaagtcgt
     8581 ccggagtatc aatatctaaa attatttctg cattttctat ttcgacggaa taatgatctc
     8641 ccataaggag tgcctgacgc atattggtga cattgggtcg ctggaatgcc tgcatcaagc
     8701 atggttttga cactaaaatc ggatggccgg ggatgccatt atggagtggc agtattgcgc
     8761 catcatttcg taacgaccag atttttctaa aaatatcaat ggtgagggtt ggcatgtcac
     8821 cgtgggtgag aaaacaatgt tctgtttgca ctgcgggtac tgcggccttg actgatgtta
     8881 gtaaaccctg ggcataatct gggttgtgaa taatagtgat attactctgg ttcgcataac
     8941 gttcgtgcag ttcattacca cgatagccgg tgactaaaat aattcggcta caaaactgca
     9001 acgcattttt gatacttgta tcaagaattg ttccctgttg ccagggtaac atcattttcc
     9061 attgccccat tcttgatgat aatccggcag cggtaattat acagtcgatg gctgacatag
     9121 tggttccatt atttataggg gctaataacg cctgataatt gcgttacttc ctgatggatt
     9181 aagttggggg taatttatcc cagagaggtc ataaagactc gtgaaaagta tagttgaccc
     9241 atcggcatta ttcattgatt taggtgcgca gaaacgtccc actgtgattt ctgttgttgg
     9301 tgctgggggg aaaaccagct tgcttttttg gctggcagaa ctgctccaga ctcgcggcag
     9361 gcgtgtgtta atcactacga cgacacatat gtttatgcca aagtcacact ggcccgtggt
     9421 tttctgtcgt gatcccgcca tgcttcctca tgcgtctctt acatccccca tttcattttg
     9481 ttttcacagc tggaaagcga accagggaaa agtgcaggga tttacgccag aagcgattga
     9541 tgcactggtg caacgaccag agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg
     9601 aatgccgtta aaagcgcctg atgagcacga accttgcata cccaaaagca gttgctgcgt
     9661 gattgctgtg atgggaggac atactttggg cgcgaaagtg agcacagaaa atgtccatcg
     9721 ctggtcgcag tttgctgata ttaccgggtt aacacctgat gcaaccttgc aactgagcga
     9781 tctcgttgcg ctggttcgcc accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg
     9841 gcgagtctgg ttcattaacc gtttttctca atgtgagaat gcgattgcac aaagcgagct
     9901 ccttcaaccg ctgcaacaac atgacgtaga ggcaatctgg ctgggcgata tacaagaaca
     9961 tcctgcaatc gcgcgcagat ttgtgaatta gcggcccggg tattccgggt tactgaaagg
    10021 atatccattg aggttggtat gaatattttc acagaggctg caaaactcga agagcaaaat
    10081 tgtccgtttg cgatggcgca aattgttgat agccgaggct cgactccccg ccattctgca
    10141 caaatgttag tgcgcgccga tggttctatc gtcggtacaa ttggtggcgg aatggttgag
    10201 cggaaggtga ttgaagagtc gcttcaggca ttgcaggaac gtaagccgcg attattccat
    10261 ggacgtatgg ctcgtaacgg tgcggatgct gtcgggtcag attgtggagg tgcaatgtca
    10321 gtatttatca gcgtccatgg tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc
    10381 aaccgggcga tagcccagag tgcggcgcta ttaggatttg atattgccgt tgctgatatt
    10441 tatcgcgaaa gcctcaatcc tgaactattc ccaccatcaa ccacgcttct ccatgctgag
    10501 tcgtttggtg cggcagtgga agcactggat attcgccctg ataattttgt cctgattgcc
    10561 acgaataatc aggatcgtga agccctcgac aaactcattg aacagcccat tgcatggttg
    10621 gggttgctgg caagtcgtcg caaggttcag cttttcctgc gtcaattgcg tgagaaaggc
    10681 gtggctgaag aacatattgc ccgtttacat gcgcccgttg gttacaacat aggtgcggaa
    10741 acgccgcagg agatcgccat cagcgtgctg gcagaaatat tacaggtgaa aaataacgcg
    10801 ccgggtgggc tgatgatgaa accttctcat ccttccggac accagctggt ggtgattcgc
    10861 ggtgcggggg atatcgccag tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg
    10921 atcatgctgg aagtggaaaa accgacagtg attcgttgta ccgtggcgtt tgcccaggcc
    10981 gtgttcgatg gcgaaatgac ggtcgaaggc gtcactgctc gcctgacaac cagctctgcg
    11041 gaagcgatga aacttaccga acgcggattc atccctgtga tggtagatcc cgcctgttca
    11101 ttgcttgatg aactgaaacc gctttgcgtg gtggacgcta ttctggcgaa acagaatttg
    11161 ggaacgcggg cagatatggc accagtaaca atcgcgcttg ggcctggctt tactgctggg
    11221 aaggattgtc atgcggtaat tgaaacaaat cgcgggcact ggctcggtca ggtgatttac
    11281 tctggttgtg cgcaggagaa taccggtgtt cccggcaata ttatggggca taccacccga
    11341 cgggtgatcc gtgctcctgc tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg
    11401 gtgaaagagg gcgatgtgat tgcctggatt ggtgagcatg aaattaaagc accgttgacg
    11461 gggatggtgc gtggcttgtt gaacgacgga ctggcagtgg tcggtggttt taaaattggt
    11521 gatatcgatc ctcgtggtga aacggctgat ttcaccagcg tttctgataa agcccgggcg
    11581 attggcggcg gcgtacttga agcgttaatg atgttgatgc atcagggcgt taaagcgacg
    11641 aaagaagtgc tggaagtggc ttaaatgaaa atggccggat ggtattaccc acccggcctg
    11701 agactgatga caaacgccaa attgcctgat gcgctacgct tatcaggcct acaagtgcat
    11761 tgcaatctat tgaatttgcg atgttttgta ggacggataa ggcgctcgcg ccgcatccag
    11821 catcaacaaa gcgcacttac ttgacaatct ttggccggat ggtattaccc acccggcctt
    11881 aaaattactg acaaatcacc gttccggttt tgccttcaat cccttctttc gctttgctca
    11941 atacggtaat cagtgcttca cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca
    12001 ctttaggcag catagaacct ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac
    12061 ttaatgacag gcgatcgagc cactgttcat tctctttacc gaagttaatg gccacttttt
    12121 ctaccgcagt cagaatgatc agcatatcgg catcaatcat ttctgctaaa cgggcgctgg
    12181 cccagtcttt atcgataacc gcgctggcac cacgcagatg gttaccttca cgaataacag
    12241 ggataccacc accgccaacg gtgatcacca cctggcctgc atctaccaga gctttaaccg
    12301 tttctttttc aatgatatca accggttttg gcgaggcaac aacacggcga tagccgcgac
    12361 cagcatcttc tttcagagtg taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa
    12421 agaacgaacc gatcggcttg gtcgggttga ggaatgccgg atcgttagcg tcgacttcga
    12481 cctgggtaac cagcgttgct acaggtttat taatgccccg agaaagcagt tcttctctta
    12541 aagcgttttg tagatcgtaa ccaatataac cctggcttaa tgcaacacag acagacatcg
    12601 gcagcattgg cgagtgtgct tcagtttttg cggcagcttc aaaggcctgg ttaatcatgc
    12661 ccacctgtgg cccgttacca tgagtgacga taacttcatg tccctgggca attaaatcaa
    12721 caattgcctg agaagtaatt tttaccgctt tcatttgccc ggccaggtca tcgcccagcg
    12781 cattcccgcc cagggcgaga acaattttct tactcatttt tcctcactca tcattcttga
    12841 ttgtgtatag atccctggca gtggttagag cacgggaggg acaaacggtt tgcgtcgcag
    12901 gaaacgacct cgcccggctt tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg
    12961 ggataaggtt ctgacaatcg cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc
    13021 gttgtcgtgg agatggcgat gctgaatttg ttggctctga cgtgggtcga taatcaccac
    13081 gtcgccatcg gaaccgggcg ctaataatcc tttttgcggc cacaggccaa acagcctggc
    13141 gggcatcgcg ctggttaatt caacaaaacg ttccggtgtt atacgtcccg tcatcacgcc
    13201 actggaaaac agtaactgca tgcggttttc cacaccgggt aagccatttg ggcagcgact
    13261 gaaatcgcct ttagaaattt gcaggcgttg agccattgag aacgtgcagt gatcggtcgc
    13321 caccacgtca atcgcaccat cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt
    13381 acgcagcggt gggctaagaa tgaacttcat gccatcttct gtatcgtagc tgcgttcgtc
    13441 caacaggaga tattgtgggc aggtttcaac ccagactggc tggtgattcg cacgggcaag
    13501 acgcagataa tccagaccta agccgttaga caggtgcacg atatagagcg gggcgttacc
    13561 ggcaatttgt gccaggttaa tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg
    13621 actcaaggcg tgatagcgcg gcgcggttaa ccctgcggcg ataaactccg cccgcttgct
    13681 ggcgatagca gcatcatttt ccgggtgcac ggtggtcagc gcgccggatt catgcaagcg
    13741 gcgtaatgcc tgcaaaactt cgtcatcgtt gagtttgtat tgataggtta aatagagttt
    13801 aaaactgctc agtccttcct cgaccatcat cgggatttcg tcgaggatgg cgtgattaat
    13861 gtgctggatc acaccgtgaa agctgtagtc gatgaccgcc ttatgggcgg catagccacg
    13921 ataaacctcc agttgatggc gtaaccgaca gccgtttggg ccaaatccca tatggtcaat
    13981 aatggttgtt gtaccgccac acgcagctgc gcgggtaccg gtaaaaaaat catcacaact
    14041 gcgcgcgatg ccgacatcaa tattgaaatg cgtatggaca tccacgccgc cagggaaaac
    14101 gtaacagcca gtggcatcaa tttcttcata cgggagctgc ggcgaaatat tgttgcccaa
    14161 ctggcgaaca atcccgcttt caatcagcaa atcctgtttg gcttgtccat ctgcgttaac
    14221 gacagtgccg tttttgatca atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg
    14281 gaggggaata agtgaagagt aattattcgg ttgccagcca gcttaacggg attgcagcgt
    14341 acatcgcggc acaggtcacc aagtgagatt tccaggtttt ttcgttaggt gcatgcgctt
    14401 caggttcttt acccgggcca aagccgatca ccggaatgcc gtgacggccc atgatagata
    14461 cgccgttagt tgagaaggtc cacttatcaa caaccggcgc tttgccaaac agcccttcgt
    14521 aggcattcac cagtgcttta acggtgaagt gatcttcttc cactttccag gtcgggaagt
    14581 agcattcggt tgggtaaacc aggccagtcc aggacggacg gtcgtagttg tacatagaaa
    14641 caaccgcgtt agctttctgt actgcaggca gggcgcggat ttcgtccagc gcgccttccc
    14701 aggtttcgcc ccaagtcaga cggcggtcaa tagagacggc gcagctatct gctacagcgc
    14761 aacggcttgg ggatgtgaag aagatttcag aaacggtgag ggtgcctttg ccgaggaatt
    14821 cgtcataacc cagacgttgg gagagttctt gtaattcgcc aagaatcgga cccattttga
    14881 aaatggcgtt gtcaccgcgt tctggcgcag aaccgtggca gctaacaccc tgaacatcaa
    14941 tacgaatttc catacgaccg cgctgaccac ggtatacctg gcagtcggtt ggttcggtac
    15001 tgaccacaaa ttccgggcga atgccggatt gttcaataat gtactgccag cacagaccgt
    15061 cgcagtcttc ttcctgaacc gtaccggtaa ccagcagggt atattcatct tccagaccga
    15121 ggtctttaat gattttaccg gcataaacca tagatgccat gccgccttcc tggtcggaag
    15181 taccgcgacc accgatcagc tcatcagttt ccatgccttc gtacggatcg aagtcccagt
    15241 ttttgatgtt gccaatgccg acggtatcga tatgagcgtc cattgccacc agacgcgggc
    15301 cgtggccgat ataaccgaga acgttgccca tcgggtcgat ttcaacttta tcgaagccga
    15361 ctttttccat ctcttcttta atacgatgta ctacgcgttt ctcgtcgcag ctttcactgg
    15421 gaatagcaac catgtcgcgc aggaagcgag tcatgtccgc ctggtaatct tttgcttttt
    15481 caagaatcag tttgaatgga atattcttag ccattatttt tctccagatt ttttactccc
    15541 cgttttgcag ggagtagttc caatagggtg attaaggtgc tacagcgtgt ttgccttccc
    15601 agacaacttc gcggtagtgc ttcacgtcgg tgtcgccttc agtgctgata accagcacca
    15661 cggcatcttt gttcagcgcc agtttttcca tcaggctttg acgttgcggg tgataatgaa
    15721 ccgccgcgag aacgcccaaa ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt
    15781 tgccgtacgg attacccagc acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga
    15841 tgaattgggt ggcgcagtta cgtaggattt cccagcccag cgggttaggt tcgccacagg
    15901 ccaggcccgc catgatggtg gccatatcac cgccaacgtt gacgatgtcg cctttgacgc
    15961 cggagcgata aatacagtca gctttgtcag gttcaacaat aatgctgtgc agattttgcg
    16021 ggctaaagac gtcgaccaga taacccagca caccaccggc cattgctccg acaccggctt
    16081 gcagcagaac gtgcgtcggg gttacgccca tttcacgcat ttgctcgacg gcttcatctg
    16141 ccagggttgc gtagccttgc atgatccagg ttggaatttt ggtgtaacct tcccatgccg
    16201 tgtcctgtac cacttcccag ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat
    16261 cgtcatagtt catatccgtg acgatgcact cggcaccgag gttcagaatg gcgtcaacgc
    16321 gttcctgagc agaacctttc ggcatgtaaa tcaccgcatt ctgtccgagt tgctgcgctg
    16381 cccacgccac accgcgcccg tggttgccgt cggtggtggt cgcgaaagtc attttttcgc
    16441 cgatggcatt tttcaggtgc tcaaatgaca gcgtttcgat atcaagatga tatttttcac
    16501 acaataattg agcgatagcg tacgcaccgc cgagcatttt gaaggcgttc agaccgaatc
    16561 gttttgattc gtctttgaca agaattttct tcacaccaaa aaggtttgcg agatcgtcca
    16621 gcgcacaaag cggtgttggg cgataaccag ctattttctg gtggaactgg cgcgccagtt
    16681 tggcctggct ttgcgaaaag agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga
    16741 tatcaatctt caatgagaaa acggacatat cctatccttt tggcctctgg aaatagaggg
    16801 ccgaccagct gatcggcccg aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt
    16861 gctcgagcag tgcacctggt ttggcgtatt tacgggacag gatcatcgcg gcgatgatat
    16921 aaggcttcca gctggcttct ttgtaggtag cgatacggta tttttcgaat acgccttcag
    16981 tcacttcacc ttctttacag gatacgccgc tgatatcagc tggcaggcag tgcatgtaca
    17041 gggcttcgcc atcacgggtc agttccatca tctcttcagt acaatgccag tctttgtgtt
    17101 gcgcgttctg tgccagacac tgtttttcca gtgcttttaa gccttcgtga tcgttcgcac
    17161 gcagcaattc agtacgctct tccatcactt tgtaaggtgc ccatgacttc ggataaacga
    17221 tgtctgcgtc tttgaaggct tcttccatgc tggtgacctg acggaagcta ccgccggagg
    17281 ctttagcatt gtttttagcg acttcaacga catccgggat caggtcgtag ccttccggat
    17341 gggccagggt gacatccata ccgaagcgag tcatcagacc gatgatgcct tgtggtacag
    17401 agagcggttt gccatagctt ggggagtagg cccaggtcat ggcgattttt ttacctttca
    17461 ggttttccaa tgaaccaaag tgttcacgta accatgccag gtcagccatt gactgagtcg
    17521 ggtggtcaat atcacattgc aggttcacta aagccggacg ttgcggcagc acaccctgct
    17581 tgtaaccgtc atcaagtgca gcgccaactt cacgcatata ggcgttgcct gcgcccagat
    17641 acatatcgtc gcgaatacca atagcgtcgg cgcagaagga gatcatattg gcggtttcac
    17701 gcacggtttc gccgtgagcg atttgtgatt tgccttcgtc gagatcttgc tgtgcaaggc
    17761 cgagcaggtt aagcgcggaa gcataagaga agcgggtacg ggtggagttg tcgcggaata
    17821 cggaaatacc taatccacta ttaaagactt tggttgagat gttttcagca cgcagtgctt
    17881 ttaatgctgc ggcaacgtcc agtacttgtt tcagttcatc tggcgtctgt tcccacgtta
    17941 acaaaaaatc tttctcgtga aggtgagagg tcagcgaatt gatatcctta atcagctcat
    18001 taacagtttt catcataaag tcccggtagt ggaatgacag gcaaagtggt ttatttcact
    18061 gtgacttgcc aaagtgattg gtgttttcac agctatacct gggactaaca ataagtgtgc
    18121 cagaattatc cacggggctg caaaagacta atttgacgga taatgtgtgg ggttgatcac
    18181 aaattgatgc tagcttccgg ttgtttgtaa aatacgaaaa cgtttggctt tttttattgc
    18241 gttatttatt tgctgctaag tatcagaaat aagataaagg ggcaggctat ccctcgtcag
    18301 tttgataaac tcaccgccgt aattctcata ttgatataga aatgtgatat ctcttccata
    18361 atttcattag aaagtgataa aaatcttatg tttttatcaa agccaatttc gcctgagtta
    18421 atatcatcat gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg
    18481 aaggtaaagc tatggagctt gctactacgc agtcagtatt gatgcaaatt caaccgacaa
    18541 ttcagcgttt tgccagaatg cttgccagcg ttttgcagct tgaggttgag atcgttgatg
    18601 aaaacttgtg tcgcgtcgcc ggaactggcg cgtatgggaa gtttcttggt cgccagttga
    18661 gcggcaactc acgcctgctc cgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac
    18721 agtctcgctt cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc cgcgaaaaag
    18781 catttctggg tacgcctgtc attttacagg atcgttgtgt tggggtgata agtttgattg
    18841 ccgttaccca cgagcaacaa gagcatatca gtgataattt acgcgaattt tctgattatg
    18901 ttcgccatat atccaccatt tttgtttcga aacttctgga ggatcagggg ccaggagata
    18961 acatcagtaa aatattcgcg accatgatcg ataatatgga tcagggcgta ttagttgttg
    19021 atgatgaaag tcgggttcag tttgttaatc agactgcctt aaaaacactt ggtattgtac
    19081 aaaataatat tattgggaaa cctatccgtt tcagaccatt aacatttgag agtaatttta
    19141 cccatggaca tatgcagcat attgtttcgt gggacgataa aagtgaatta atcattggcc
    19201 aattgcataa cattcagggc cgacaattat ttttaatggc gtttcaccaa tcgcatacca
    19261 gtttttctgt agcaaatgca cctgatgaac cgcatattga acaattggtt ggcgagtgcc
    19321 gtgttatgcg gcaattaaaa cgactcatta gccgtattgc acccagccca tccagcgtta
    19381 tggtggttgg tgaaagcggc acgggtaaag aagtcgtcgc ccgtgcaatc cataagttga
    19441 gcggaagacg gaataaaccc tttattgcta tcaactgtgc cgcgattccg gagcagcttc
    19501 tggagagcga actgttcggt tatgttaaag gcgcatttac tggcgcttct gccaacggta
    19561 aaacagggtt gattcaggcg gcgaatacgg gcacgctgtt tctcgatgaa ataggtgata
    19621 tgccattaat gttgcaggct aaattactgc gcgctattga ggcacgtgaa attctgccga
    19681 ttggtgccag tagcccaata caagtcgaca ttcgcatcat ttctgcaact aaccagaatt
    19741 tggcccagtt cattgccgaa ggtaaattcc gcgaagatct cttctaccga cttaatgtta
    19801 tcccgataac tctgccaccg ctgcgtgaac gtcaggaaga tattgaacta ctggtgcatt
    19861 actttttaca tctgcatacc cgtcgtctgg gatcggttta tcctggcatt gctcccgatg
    19921 tcgtcgaaat attgcgtaag catcgttggc ccggaaacct gcgcgagtta agcaatttga
    19981 tggaatatct ggttaacgtt gttccttcag gtgaagttat cgacagcacg ctattgccgc
    20041 caaatctgct gaataatggc acaacggagc aaagtgatgt aacagaggtc actgaggcac
    20101 acctgtcact cgatgatgcg ggcggcacgg cgctggagga gatggaaaag caaatgatcc
    20161 gcgaggcgct ttcacgtcat aacagcaaga agcaagttgc tgatgaactg ggcatcggca
    20221 ttgctacgct ctatcgcaag attaagaaat atgagttgtt aaacacataa catcagtgaa
    20281 tccggcctga attcaggccg gatatccttt tacttcgttt tctcgcaatc cagaactgta
    20341 tttacaatca tctgataccc cgtgcagcga caaagatttc ccgccagtcc gcgacgaatt
    20401 tccgtaatgg ttaatggctt ctcgcgtggt ttcgccagca ttgccgtggt agccataatc
    20461 aggccaggcg tacaaaaccc gcactgcact gcgccggatt tcgcataagc ctgctgaaca
    20521 tgagaaagtt ttccgccttt cgcttcacct tccagcgtgc ggatctcttt tccttcagcc
    20581 caggcggcaa ggtataagca actgtctatt gctgtgccgt cgaccagcac cgtacaggca
    20641 ccacattcac ccacgcagca cccttgtttg acacttagca gtccttgttc gcggagtaat
    20701 tccgagagcg gcgtgcctgg tgcggcatga agctgaaaag gcatcccgtt aatggtgcat
    20761 tcgatggtaa ttgtttcgct gtgattcatt gcaattttcc ccccgccgcg gcgacggctt
    20821 cgctaatcac ttttttggtc atcgtctgga tgagatgcag acgaaactct ttactggccc
    20881 gccatgaaga acgcggggcg acatcttgca ggacagattc gctgatagct tccagcgttt
    20941 gcaggtttaa tggcgcattt tgtgcagtct gttcggcatg ttggcagcga atcggcgttg
    21001 gcgcggcaac accaaatgcc agacgtaatt cgctgaaatt gccgttatcc agtcggcaat
    21061 gtgcggcgca gccaatcgtt gaaatatcca ttgcgtcgcg catggcatat ttaaaatgcg
    21121 cgctgcccgc gtgttctttc ggctggggcg gaaaatgaaa ggcgacaagg atttcgtcat
    21181 gctcaagaga cactttgcct ggcccagtgt gaaagccatt aatcgggacg aaacgaacac
    21241 cgcgtgggga gtggatctcc agctttgcgt cataaattag cgttggcgtg gcagaatctg
    21301 cgctggtggc accgttgcaa atatttccac cgtaggtagc gacgttacgg atctgcggtc
    21361 cggcaatgga cgaggccgca gcacataacg ccgggagatg acgttgagtt atagaatctt
    21421 ctattagctg ggtaaatgtc gttgcagagc cgatacgtag cgagccatct tccgccagcg
    21481 taattccccg cagctccgcc agattatgaa tatcaacaat atggcgataa cggtcattgt
    21541 gatggtggag ctgaatcagt acgtcagtgc caccggcgag cagtttggcc tgcgggttgt
    21601 cagccagcag gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa gcaaaatcaa
    21661 acatgatgtt atcctcaaat caatcctgcc agatgaaact cttcatataa ccgttttggc
    21721 gtcagcggca gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt acgaatagct
    21781 gcggcaacag gaattattgg tggctcaccc agtgacttat gtccgtatgc ggattgcggt
    21841 tcattgattt cgacgaacgc gctttccagt tgtggcagat ccggcatggt cggcattttg
    21901 taatccagca gattggggtt acggaccacg ccgcttttcg catcgatgat catctcttca
    21961 aatagcgccc agccaatgcc cattcccatt ccgccgtgta cctgaccttc tgccagcagc
    22021 ggatttagaa tatgccccga atcatgaacg ttgaggatgc ggttgatggt gactttgcac
    22081 agtgcaatat cgaccgtcag atcaacaaag gtacagccaa acgccggtgg gttagtggtg
    22141 gttttgatgg agctttcagc agagagctgc ccgccgcgtt cagggtggta gaaagcgtcc
    22201 atcgccaaat cttttaacga cattaacggc tcttccggtc gttcaaccag cacgatatgg
    22261 ccttttatca gggtcagatt catcgctgac tgatgtagca tgactgcggc gtgagcgatg
    22321 attttctctt ttaataacag tgccgcactg cgcagcgcag gcgcggcaac atagctctgg
    22381 cgtgaggcaa atgcgccggg atcgaacggc gtaacgtcgg tatcttgtgt tgaaataacg
    22441 cgaacgtcgc taaccggaac ccccacggtt tctgccacca tttgcgagaa gacggtgtcg
    22501 gcaccctgac cgatttccgt cgcgccgctt tgcacgttga tggttccatc ctgattcatc
    22561 agaaggcgcg cgcctgctat ttctacgccg acaggccagg tattagaggt gtagctaaaa
    22621 caggcgacgc caacgccgcg gcgcaaattg ccttgctggt tctggcattc tgcacggcgt
    22681 ttttcccatt caaagatttt ccggcctttt tcaagacact ccggcaaccc tgcgctgtaa
    22741 atacgtttgc ccgtgagcgg attagcatct ccttcgcggg cggcgttgcg taaacgaatt
    22801 tcaacaggat caatacctaa cgctgtcgcg gcgtcatcaa gcatagactc aacggcaaat
    22861 acgacttgtg gcgcgccata accacgcatc gcaccagccg aggggaggtt ggtatagcag
    22921 gtctttgaac tgtaagcgta ggcacaacga ggataaaggt aagcgacttt attccccccc
    22981 gcagaagcga tggagtgccc gtgagatgca taagcgccgg tgttagacag aacatccaga
    23041 ctataacctt tcaatgttcc gtcgcggctc acgcccattt gcccgtcaat agtaaaagcg
    23101 tggcgggtac gggttgcgag gaaacactct tcacggctaa gggaaacttt caccggaatg
    23161 ccgccaagct tgctggtcag gaatgccgcc attggctctt ccagtacatc ctgtttatta
    23221 ccaaaaccgc caccgacaaa tggtttgatg actcgtacgc atgaccaggg aatatccagc
    23281 gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg tgctggaaac gatggtaatt
    23341 cgcgagtcat cctccatcca cgccagcgat gttacgcttt ccatgtgaca atgttgaata
    23401 acgggggtct gatagtgccc ctgtacctgg tagtcggcgg catcgattgt ttgttggaca
    23461 ttgcccgtcg acatcgtgct ttgtttcagt aaattgccgc cgttatggat tggtgcagcg
    23521 tcctctgcca gcgccgcttc tggcgtggtg ataacgggta attcttgcca ctcaatgctg
    23581 accaattgcg ccgctttttc tgccgtgagt tcatcgcggg ccacgacgat ggcaacggcg
    23641 tcaccatgat gacgaacatg gcgagttagc agtgcgcgat cggcggtatc gcgcttgttt
    23701 tcgtcaagtg tccatgcatg cccagctgta gcgaatggga tatcaggcac atcttcccag
    23761 gtaaaaatcg ccagtacgcc tggtaaactt ctggcttgtt catcattaat acttacggca
    23821 taaccatgtg cgatagggct acgtacatat ttcgcataac acatgcccgc cataacataa
    23881 tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg catcaacgcg catgcatgat
    23941 tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg aacatttggt
    24001 tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacga cgataagcgt
    24061 taggggagga aggaagtgtg atgacgttaa aaaaacacag atatgatacg aaacaggact
    24121 ctgaataggg gcagtgatac gtatcaatat gagtggcgat aaaatcaatg tgataaattg
    24181 ggattattac gcatcaggat gggaaatagg gtaaaaaagt caggaaattc gatacctcta
    24241 cagcgaaaat tttgctgatg atacttccct ttgccaaatg aaattaacct ctttctctat
    24301 taccgcgttt ttccagaagc aagagattgg gtatcaagag gctggctgct atgatatggc
    24361 gtcttgtttt ttaagcgagg aaagattttg agtgcgggac gcctgaataa aaaatctctg
    24421 ggtatcgtga tgttgttatc ggttggactg cttttggcgg gctgttcggg tagcaaatca
    24481 tccgatacag gaacgtattc cggctccgtt tacaccgtga aacgggggga tacgctatat
    24541 cgtatttcgc gcaccacggg aaccagcgta aaagagctgg cgcgactgaa cggcatttcc
    24601 cccccttaca ccattgaagt tggtcagaaa ctaaaactgg gtggggcgaa aagtagcagt
    24661 agtacacgta aatcaaccgc caaatcaacg accaaaaccg catcggttac accgtcatca
    24721 gcggtaccga aatcttcctg gccgccagta gggcaacgtt gttggttatg gccaacgaca
    24781 gggaaagtta tcatgccgta ttcgacagca gatggcggca ataaagggat tgatatctct
    24841 gctccacggg gtacacctat ttacgccgcg ggtgcaggaa aggtggtgta tgtgggcaac
    24901 cagctgcgtg gctacggtaa tctcatcatg attaaacaca gtgaagatta cattacggct
    24961 tacgcccata atgacacgat gctggtaaat aatgggcaaa gcgtgaaggc tgggcaaaaa
    25021 atcgccacca tggggagcac ggatgcggca tctgttcgcc tgcatttcca gattcgttac
    25081 cgtgcaacgg caattgatcc gctacgttac ttgccgccgc agggcagcaa gccaaaatgc
    25141 tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc taaggagagc gtaaggttta
    25201 taatgcctta cgcatctcaa agcgggcgta gttcaatggt agaacgagag cttcccaagc
    25261 tctatacgag ggttcgattc ccttcgcccg ctccaattta tcttcacctc atcaacctgc
    25321 catttttgtt gtatctgatt ttctatatta tcttaattca atcggttcag acttttcgca
    25381 atcgcagtaa cacttcccca tttttttgta aataaaaagc gtttacgcaa tgcaatcctt
    25441 ttataacaac ccaggtaaaa taccttattt tcaagttgtt aaataagtgg ttatctacgc
    25501 ttttaacaaa cgattgatgg aatattttat gaaagggaaa tctgcattaa ctcttctgct
    25561 cgctggtata ttttcgtgcg gcacatgcca ggcgacaggg gcagaagtga cgagtgaatc
    25621 tgtatttaac attcttaatt ccacgggggc agcaacagat aaaagttatt tatcgttaaa
    25681 tccagataag tatcctaatt atcgtcttct gattcactca gccaaattac aaaatgaaat
    25741 aaagagccac tataccaaag acgaaattca aggattgcta acattaacag aaaacacacg
    25801 aaagttaacg ttgacggaga aaccctgggg aacgtttatt cttgcttcta ctttcgaaga
    25861 tgacaaaaca gcggcagaaa cccactatga tgctgtctgg ctgagggata gtttatgggg
    25921 ctatatggca ttagtatcag accagggaaa tagtgtagcc gcaaaaaagg ttctgctaac
    25981 cctgtgggat tatatgtcta cgcccgacca gattaagcgg atgcaggaca tcattagcaa
    26041 cccaaaacga ctggatggta ttcccgggca aatgaatgct gtgcatatcc gatttgacag
    26101 caactccccc gtgatggcag atgtgcagga agaaggcaag ccgcagctgt ggaaccataa
    26161 acaaaatgat gcgttaggtc tttatcttga tctccttata caagcaatca atactggtac
    26221 tattaacgct gaagattggc aaaaaggtga ccgactaaaa tcggttgcgc tgctgattgc
    26281 ttatctggat aaagccaatt tctatgttat ggaagattcc ggagcgtggg aggaagatgc
    26341 ccgattaaac acatcttccg ttgcactggt gacctctgga ctggaacgtt tgtcaaacct
    26401 actctcgaag aaagactctg tttttgtctc tgatttacta cgcgaagcta aagtcaatga
    26461 actggatgaa actctttcaa caacgaggtt gaaccacctc atagataaag gttatgaaag
    26521 aataacgtta cagcttgatt tagggggaga atccccaggt tatctggaaa aagataaaca
    26581 ttacagagaa gccgatgcgg ctttacttaa tgttatttat cctgcaaatc tttcaaagat
    26641 aaacaccaga agaaaagagc aagttctaaa aatagttaaa aaactagcag gtccgtatgg
    26701 tataaaaaga tacgagaagg ataattatca gtcggcaaat ttttggttca atgatattaa
    26761 aaccgatacc gatcaaaatt cgcatgcaaa aagagaaaag agcttcattc catcaacaga
    26821 agcagaatgg ttttttgact catggtatgc aaaatcagca gcaattgttt ataaagaatc
    26881 aagaaaagaa gagtatttga atgactcagt acaatttatg aatagatcat tggcccaaat
    26941 aacaggcgag aatatgattg gtgcaaatgg caggagcgta ccagaaatgg cacttcccga
    27001 aagctataac tatatccaca agtcgggaac tttgcatgaa gcgccaagcc ctatcattcc
    27061 gctaaattgg tcgaaagctt caatgactct catgttgaaa gagatgtcca accttatcaa
    27121 tgatgaggga attaaatagt tttcataatg aggcggtcct ggattaaaag gaccgctcaa
    27181 agagaaatcg aaaggggagg taaaagtgat caataaatta ctgttagctt acctgatagg
    27241 tctggttgtg acatcgactt taatatttat cttctcggaa gaaaaagtaa catatcggtt
    27301 gtttgctgca ataattacag gtttaacctg gccgcttagt ctcattcctt ccattataag
    27361 tctcatgatt c
//
LOCUS       DAZGBC010000055        27217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGBC010000055 DAZGBC010000000
VERSION     DAZGBC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.055"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..20824
                     /locus_tag="ACHK1J_002279"
     CDS             47..20824
                     /locus_tag="ACHK1J_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO7911534.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTTVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDTTGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGEVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVDAL
                     ADGNATVTASVNDVAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHIQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSSIGNSTTASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAAVGDAVTVTLGGNNYTTTVQAGLSWSVD
                     VPADAIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSLGLAEGTPLTVTINDVEYTTAVQADGSWSVGITAAQVSAWPAGAVNI
                     AVSGESSAGNPISITHPVTVDLTPAAITINTIATDDVINAAEKGANLTLSGTTTNVEA
                     GQTVTVTFGGKNYTASVAGDGSWTATVPAADLAALPEGSASAQASVSNINGNSASAVH
                     SYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDVINATEKGAD
                     LQISGTSNQPAGTTITLTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVT
                     DSAGNSNSASHNVQVNTALPGVTLNPVASDDIINAAESGVAQTISGQVTGAAAGDTVT
                     VTLGGKTYTATVQGNLSWSVDVPAADIQAIGNGELTVNAWVTNGVGNTGSGSRDITID
                     ANLPGLRVDTVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLADG
                     TWSLGVPAANVGNWPAGTVNITVSGATSAGNPVTITHPVTVDLAAVAISINTVSGDDV
                     INAAEKGADLTLSGSTSGVEPGQTVTVTFDGKTYTATVAGDGSWTTTVPAADLAALRD
                     GDATVQASVSTINGNTASATHVYSVDATTPTLTINTIATDDILNAAEAGNPLTISGSS
                     TAEAGQTVTVTLNGVTYTGTVQADGSWSVSVPTADLSNLTASQYTVSASVNDKAGNPV
                     SATHGLAVDLTVPVLTINTVSGDDIINATEHGQALVISGSSTGGEAGDVITVTLNSKT
                     YTTTLDASGNWSVGVPLSDVTALGSGPQNITATITDAAGNSDDASRTVTVNLAAPTIG
                     INTIATDDVINATEKGADLQITGTSNQPAGTTITVTLNGQNYTATTDASGNWSATVPA
                     SAVSALGEANYTVTANVTDKAGNSNSASHNVLVNSALPAVTINAVATDDIINADESDN
                     AQTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSISVPAADIQALGNGDLTINAL
                     VTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSTGLTAGTA
                     LTVEINNVTYAATVLADGTWSLGVPAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVT
                     VDLAAVAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEA
                     NGSWTVNVPPADLAALPDGAGNVQASVSNINGNNAQADRAYSVDATAPLVTINTIASD
                     DTLNVSEAGAGITISGTTTAQAGQTLTVTLNNNTYQTTVQADGTWSVNVPAADLSGLT
                     ASSYTVTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGT
                     STGAAAGDVVTVNLNGKNYTTTLDASGNWSVGIPAADVTALATGSQTITASLSDRAGN
                     SDSTTHNVTVDLSGPTLTISIVSGDDIINNAEKTQDLIISGGSSGLATGTTVTVMLNG
                     LAYSATTDGSGNWSVTVPASAVGALGEAVYQISASATDSAGNSGITTHTVNVESLLPG
                     VIINTVAGDDIINAAEIGVNQTISGQVTGTAAAGDTVTVTLGGNQYVATVQPDLSWSV
                     SVPANVLQALGNGELTISASVTNSANNTGTTTHDIVIDANLPGLRVDTVAGDDVINSI
                     EHTQALVITGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPAADVTNWPAGTVN
                     IAVSGTNTAGTTTSITHPVTVDLAAVAITINPLSTDDVINAAEKGSDLQLSGTTSDVE
                     AGQTITVIFGGKSYTTTVAADNTWGLTIPAADLAALPDGAANVQASVSSVAGNSAQAT
                     HVYSVDATAPSVTINTIAADDILNAAEAGSALIISGTSTTEAGQTVTVTLNGVNYSGN
                     VQADGSWSVSVPTGDLANLTASSYTVNASVSDKAGNPASATHNLTVDLAAPVVTINTV
                     AGDDIINATEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVI
                     SALAQGDVNISATVTDSAGNSGTASHVVSVALGAPVLAINTIAVDDIINATEKGADLA
                     ISGTSDQPAGTQITVTLNGQNYTTTADASGNWSVTVPASAVGTLGEATYTVTASATDA
                     DGNSGSASHNVQVNTALPGVTINVVATDDIINAAEAGVDQTISGQVTGAAAGDTVTVT
                     LGGATYTATVQANLSWSVDVPASALQALGNGELTISASVTNSVGNTGNGTREITIDAN
                     LPGLRVDTVAGDDVVNIIEHGQALVITGSSSDLAAGSNVTLTINGQTYVAAVLADGSW
                     SVGVPAADVSAWPAGTVTITASGNTTAGNPVSVTHPVTVDLTAVAVSINAITVDDVIN
                     AAEKGAALTLSGSTSGVEAGQTVTVNFGGKTYTASVATNGSWSTTVPAADMAALRDGD
                     ASAQASVSNVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTA
                     EAGQTVTVTLNGENYTGTVQTDGSWSVSVPQADVSALTASNYTVSAAVNDKAGNPASV
                     NHNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFT
                     TVLDASGNWSVGVPASVVSALANGTVTINASVTDVAGNSGSATHQVTVNTGLPTITFN
                     AISGDNVLNADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVS
                     DLAALGQANYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGAD
                     QTISGVVTRAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASV
                     TNANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVL
                     TVTINSVAYSATVQADGSWSVGIPAANVSAWPAGPLTVEVAGQSSADNPVSVSHPFTV
                     DLTAVAISINTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAAN
                     GSWSKTVPVADLATLPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDD
                     ILNATEAGSPLTISGSSTAETGQTVTVTLNGATYTGTVQADGGWSVSVPTSALGALNA
                     SNYTVSATVNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTS
                     TGGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAGNS
                     DDASRTVTVSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQN
                     YSATTDASGNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGIT
                     INPVATDDIINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQPDLSWNVSVP
                     AADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHS
                     QAQVITGSSSGFAAGTALTVVINNQTYAATVLVNGTWSVGVPAADVSNWPAGTLNITV
                     SGANSAGTQTSITHPLTVDLTTVAISINAITPDDVINAAEKGAALTLSGSTSGVEAGQ
                     AVTVTFGGKTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEY
                     SVDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQA
                     DGSWSLTLPASDLTALANNGYTLTATVSDLAGNPGSASKGVTVDTTAPVISFNTVAGD
                     DVINNVEHTQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGL
                     ADGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLSG
                     TGTNFATGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSA
                     GNSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFG
                     GKTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAP
                     TVTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWSATVGSGGSWSVF
                     IPAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAE
                     HGSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVI
                     NASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSL
                     TAALASNETAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGA
                     TDSQNVVIDTTAPDPAVKTIAISAITTDTGLITNDFVTSDTTLAVSGTLGATLSAGEF
                     AQISIDGGTTWQNLSVSGLTWSYLDGRTLTDGNYNYQVRVIDTAGNIGATASQIVTVD
                     TTAPLASKTIAIASISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVT
                     WTTLTVIGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSI
                     TITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTLAADG
                     LNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATGSQSAQIDTVNPAQVLTIASISTDTG
                     SSATDFITSDTSLTLTGSLGAGLASGEVAQISLDGGATWTTLTTNGTQWTYTDSRTLT
                     DGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPGIVSYTDDVGQRQGTLSSSQAT
                     DDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSAR
                     VVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVV
                     INGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANI
                     SNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKT
                     ALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTV
                     GTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATV
                     GGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTN
                     TFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAV
                     GATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVGGASNWTQSALGGSQSGT
                     TSGVAAMDYDWDGAVDVLVTKQSGSVFLIRNTNTVSYGTSLHLRITDPNGINVYYGNT
                     VKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNID
                     QTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGW
                     VYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFN
                     DTLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTW
                     EGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQ
                     IDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            20828..22990
                     /locus_tag="ACHK1J_002280"
     CDS             20828..22990
                     /locus_tag="ACHK1J_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO7911535.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIVATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            22987..24162
                     /locus_tag="ACHK1J_002281"
     CDS             22987..24162
                     /locus_tag="ACHK1J_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO7911536.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            24159..24566
                     /gene="cueR"
                     /locus_tag="ACHK1J_002282"
     CDS             24159..24566
                     /gene="cueR"
                     /locus_tag="ACHK1J_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO7911537.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(24570..24773)
                     /locus_tag="ACHK1J_002283"
     CDS             complement(24570..24773)
                     /locus_tag="ACHK1J_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911538.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIN"
     gene            complement(24770..25192)
                     /locus_tag="ACHK1J_002284"
     CDS             complement(24770..25192)
                     /locus_tag="ACHK1J_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911539.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(25293..27032)
                     /locus_tag="ACHK1J_002285"
     CDS             complement(25293..27032)
                     /locus_tag="ACHK1J_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7911540.1"
                     /translation="MKSVKLSLLAVVVATIVSPSAFADDTVEAAATELTAIHPGMSHD
                     EIDQKINRFMIRTGNTEAAQDYLFSHGYMDPQTGDNIYPASTAVQTDSNITDQKQADR
                     DAAQDTAIQNAHNIASIAAANADDAKKALTTAQSAIDANKGAIADTQKDVADVKASAA
                     HANTNANTALMNGLKLSGAVSDNKNQIEKNSSSIAEQQEQMALSEQNQIHRDAAQDSS
                     IQDAQHSANWASLKADDAHHAIMVAQTDIDANKSAISDTQKDVAAVRTDVSAMKDDVA
                     DVKASADHANANANTALMNGQKLSGVVSDNKNQIEKNSTAITEQQEQITATQKTVAAT
                     GDVKTAARYQSMINALQTATADAQQSQSEAQQTQITEQQKQIAATEKTVAAIGDPQST
                     AHYQKMINAKLTAQNEANERSTADQEQRINELASNVATQQQIDSVQYGEQIRNLAQDS
                     AQAHEQIESLTQDSAQTHQQLTNTQKRVADNSQQINSLNNNFSSLKHEVEDNRKEANA
                     GIASAVAIASQPQVKTGDFMMVSAGAGTFNNESAVSVGASFNAGIHTVIKAGVSADTQ
                     SDFGAGVGVGYSF"
BASE COUNT         6146 a         7958 c         7997 g         5116 t
ORIGIN      
        1 taaatggact cgctaaggat gtgcgccatt tatcaggaat gccattatga gcctgattat
       61 tgatgttatt tcgcgtaaaa catccgtcaa acaaacgctg attaatcctg gcgacgtcac
      121 ggttgttatt tatgagcctt ccgtggtgca ggttcatgct caggcctctg ccgttgcgcg
      181 ttacgtccgt gaaggaaatg acctgctgat ctatatgcag gacggcacgg tgatccgctg
      241 caacggttat ttcctgcaag cggcgaatac agctgaacaa tcggaactgg tgtttgccga
      301 tggtcaacag ctaacccata tcacctttgc cgatactgct gcgggtggat tagcccccgt
      361 agaactgact gcccagacca ctgcgattga aagcattgcg ccatttcttg ataccgttgc
      421 tcagaccagc gctttcccgt ggggttggct ggcgggggcg gcggtaggtg gtggcgcgct
      481 tggtgcactg ctggcaagcg gtggcgatgg cgactcgaaa acagaagtga ttaataaccc
      541 tacgccaccc gctgagcctg gcaacgccac accatcattt ttagttaccg ataatcaggg
      601 cgatcagcgc ggcattctcg ccaccaatga catcaccgat gacaccacgc caacctttag
      661 cggcagcggg caggcggggg cgactattca gattaaagac agtaacggca atactattgc
      721 cagtactcag gtagacaaca acggtcactg gagtgtctcg ttacccacgc aaagtgcagg
      781 tgaacatacc tggtcagtgg tgcaaattgt cggcagtacc atcactgacg ccggttcgat
      841 aacgttaacc atcgacaata gtcaggccag cgtgcaggtt gccaccaccg caggcgataa
      901 cattattaac gccagcgaac aggccgccgg gtttacgctt tctggcacca gtagccatct
      961 ggcgcaggga acagaactca ccgtcacact taacggcaaa acctacacca caactgtagg
     1021 ggctaacggt gcctggagcg tgcaggtgcc gaccgccgat gcacaggcgt taggcgaagg
     1081 gaatcaggcg gtactggtca gtgggaaaga caccacaggc aatacggtca ccggcgcgca
     1141 gctgctaacg gtcgataccc aaccgccaac gcttgccatc aacaccatcg cccaggacaa
     1201 catcatcagt gctgcggaac ataacgtcgc gctggtactg agcggcacgt cgaatgcaga
     1261 agcggggcaa accgtaacac tgaccgtcaa cgggaaaagc catacagcaa ccgtcggtag
     1321 cgacggaacc tggcaagtga cgctgcctgc cacggaagtc caggcactgg cggagggtaa
     1381 ttacgctgtc aatgccagtg tcagcgatcg ggcagggaac accaccagcc acagcgcgaa
     1441 tttcacggta gacacctcag cacccgtggt cagtgttaat accgtggcgg gcgacgatat
     1501 tcttaataat gccgagcagg ccgtcgcgca gatcatctcc ggacaagtta gcggtgcttc
     1561 tcctggcgat acggtaacgg tgaaattggg cactcatgtc ctgacgggca tcgtgctggc
     1621 agatggcagc tggaatgtgg cgttggaccc ggcggtaacc cgcacgctgg atcgcggagc
     1681 caatacgatt ttcgtcaccg tgacagatgc tgcaggaaat actggcgcgg cgtctcgagc
     1741 aatcacgctg gtcggtgttt ctccgttgat caccattaac accgtctccg gcgatgacat
     1801 tatcagtggc gcagaaaaag gtgcgccact gacccttacc ggtagcactc aacaggctga
     1861 gacaggacaa accgtcacag taaccctggc tggacagagt tttaccacta ccgtgcaggc
     1921 cgatggctcc tggagtctga cggtacctgc cgccgcgatg ggaaatctgc ctgacggcga
     1981 ggtggcgatt accgcttctg tgacggatct cagcggcaat accggcaaca cctcccgcac
     2041 cattaccgtc gacagccagg ccccggcctt aagcattgat ccactgaccg ctgataacat
     2101 cattaacgcc gccgaaagcg ggcaggatct gcccatcacc ggcaccaccg acgcccagcc
     2161 agggcagacg gtaacggtga cgcttaatgg gcagacgtat cagggcgtcg tgcagccaga
     2221 cggcacctgg agcgtgaccg ttcccgccgc caacgttgac gcgctggctg acggtaacgc
     2281 cacggtcacc gccagcgtga acgatgtcgc cggtaacccg accagcgtct cacgcgtggc
     2341 gctggtcgat gccacgccgc cggtggtgac cattaatccg gtggcgaccg ataatgtcat
     2401 taacacgccg gaacatatcc aggcgcaaat tatcagcggc acggtcactg gcgctcaggc
     2461 gggcgatatc gtcaccgtga cgctgaataa tgtggattac accacggtgg tggatggttc
     2521 cggcaactgg agtctgggcg ttccggcctc ggtggtcagt gggctggcgg acggcagtta
     2581 tcctgtcagc gtctcggtaa ccgacaaagc cggaaacacg ggcagccagt cattgaccgt
     2641 cacggtcaat accgccgcgc cccttatcgg cattaacagc attgcgggcg atgatgtgat
     2701 taacgccagc gaaaaagggg ccgatctcca gattaccggc accagcgatc agcctgttaa
     2761 caccgccatc accgtgacgc tgaacgggca aaattacacc accacgaccg acgcctccgg
     2821 caactggagc gtcaccgttc cggcatcggc ggtaaccgca ctgggccagg ccaactacac
     2881 ggtaacggcg gcggtgacca gcagtatcgg caatagcacc accgccagcc ataacgtgct
     2941 ggtcgacagc gcgctgcccg gcgtgaccat taacccggtg gcaaccgacg atattattaa
     3001 cgccgccgaa gcgggcgtgg cgcaaaccat cagcgggcag gtgacaggag ccgccgttgg
     3061 cgacgcggta accgttacgc tgggtggcaa taactacacc accaccgtgc aggccggctt
     3121 aagctggagc gtggacgttc cggcggacgc tattcaggcg ctgggtaatg gcgatttaac
     3181 ggttaatgcc tcggtcacca atcaaaacgg caacaccggc agcggcacgc gggatatcac
     3241 catcgacgcc aatctgcccg gcctgcgggt cgatacggtg gcgggcgatg atgtggtcaa
     3301 tatcatcgag cacgggcagg cgctggtggt caccggcagc agcttggggc ttgctgaagg
     3361 cacgccgctt accgtcacga ttaacgatgt ggaatacacc actgcggtgc aggccgatgg
     3421 cagctggagc gtgggcatca cggcggcgca ggttagcgcc tggcctgcgg gggcggttaa
     3481 tattgccgta tccggggaaa gtagcgccgg aaatccgata agcattacgc atccggtaac
     3541 ggtggatctc actccggcgg cgatcaccat caacaccatc gccacggatg acgtgattaa
     3601 cgctgcagaa aaaggggcta atttaactct ttccggcacc accactaacg tagaagcagg
     3661 gcaaaccgtc actgtcacct ttggcggtaa aaattacacc gccagcgtgg cgggcgatgg
     3721 tagctggact gccaccgttc ccgccgccga tctggcagca ttaccagagg gcagcgcctc
     3781 cgcgcaggcc agcgtcagca atatcaacgg caatagcgcc tcggcggtgc acagctacag
     3841 cgtcgacagc agcgcgccaa ccatcattat caataccgtc gccagcgaca atatcgtcaa
     3901 cgccagcgaa gccgatgcgg gtgtgacggt gagcggcagc accaccgctg aagcggggca
     3961 gattgttacg gtaacgctca atagcccgac cgtgcagacg tatcaggcaa cggtgcaggc
     4021 ggacggcagc tggagcatca atattccggc ggcagatctt gaggcattga ccgatggcag
     4081 ccacaccctg accgccacgg tcaatgacaa agcgggcaac ccggcgagca ccacgcataa
     4141 tctggcggtg gatctcaccg ttccggtact caccatcaac accattgcgg gcgatgacat
     4201 tataaacgcc accgaacacg ggcaggcgct ggtgatttcc ggttccagca ctggcggaga
     4261 agcgggagat gtcgtcaccg tcacgctaaa cagtaaaacc tacaccacga cgctggatgc
     4321 ctccggcaac tggagcgtcg gcgttccggc ggcggatgtc acggcgctgg gtagcggccc
     4381 gcaaaccgtg actgcgaccg ttaccgatgc cgccggtaac agcgatagtg agacgcacac
     4441 ggtcaccgtt aaccttaccg cgccgaccat tggcattaac accatcgcca ccgatgacgt
     4501 gattaacgcc accgaaaaag gcgcggatct gcaaatttcc ggcaccagta atcagccagc
     4561 aggcaccaca attacgctga cgctgaacgg gcaaaattac actgccacca cagatgcttc
     4621 cggcaactgg agcaccacag tgccagcttc cgcagtcggt gcgctgggcg aagccagcta
     4681 cacggtgacg gcgaacgtca ccgacagcgc gggcaacagc aactccgcca gccataacgt
     4741 gcaggtgaat accgcgctgc ctggcgtcac ccttaatccg gtagcgagcg acgatattat
     4801 caacgctgcc gaatcgggcg tggcgcagac catcagcggc caggtcacgg gcgcggcggc
     4861 gggcgatacg gttaccgtaa cgcttggcgg gaaaacctac accgctaccg tgcaggggaa
     4921 tttaagctgg agcgtggacg ttccggcggc ggatattcag gccatcggca atggcgagct
     4981 gacggttaac gcctgggtaa ccaacggcgt tggcaatacc ggcagcggtt cgcgcgatat
     5041 tactatcgac gccaacctgc ctggcctgcg cgtcgatacc gtggcgggcg atgatgtggt
     5101 taacagcatc gaacacggtc aggcactggt gataactggt agcagcagcg ggctggcggc
     5161 gggcgctgcg ctgacggtgg tgatcaataa cgtcacctat ggcgcgacag tattagccga
     5221 tggcacatgg agccttggcg ttccggcggc aaatgtaggc aactggcctg cgggtacggt
     5281 gaatatcacg gtttcaggtg ctactagcgc aggaaacccg gtcaccatca cccatccagt
     5341 caccgtcgat ctggcagctg tggcgatctc cattaacacc gtttccggcg acgatgtgat
     5401 taacgccgcc gaaaaagggg cagatttaac cctttccggc agcacctccg gcgtggagcc
     5461 agggcaaacg gtcaccgtca cctttgacgg caaaacctac accgccaccg tagcaggcga
     5521 tggtagctgg acaaccaccg tccctgccgc cgatctcgcc gcattacgcg acggcgacgc
     5581 cacggtacag gccagcgtca gcactattaa cggcaacacg gcttcggcaa cccacgtcta
     5641 cagcgtcgat gccacgaccc cgacgcttac cattaacacc atcgccaccg acgatattct
     5701 gaacgccgcc gaggcgggta atccgctaac catcagcggc agcagtaccg ccgaagcggg
     5761 gcagacggta accgtcacgc ttaatggcgt gacttacacc ggcaccgttc aggcggacgg
     5821 tagctggagc gtcagcgtgc cgacggcgga tctcagcaat ctgaccgcca gccagtacac
     5881 cgtcagcgcc tcggtaaacg ataaagcggg taacccggtg tccgccactc acgggctggc
     5941 ggtggatctc accgtgccgg tgctgaccat taacaccgtc tcgggcgatg acattattaa
     6001 cgctactgaa cacgggcagg cgctggtgat ctccggttcc agtactggcg gcgaagcggg
     6061 cgatgtcatc accgtcacgt taaacagtaa aacctacacc accacgctgg acgcttccgg
     6121 caactggagc gtcggcgttc cgctgtctga tgtcaccgcg cttggcagcg gcccgcaaaa
     6181 catcacggcc acaattaccg acgcggcagg caacagcgat gacgccagcc gcacggtcac
     6241 cgtgaatctc gccgcgccaa ccattggcat caacaccatc gccaccgatg acgtgattaa
     6301 cgccacggaa aaaggcgcgg atctgcaaat taccggtacc agtaatcagc cagcgggcac
     6361 cacgataacg gtgacgctga acgggcaaaa ttacaccgcc accacagatg cttccggcaa
     6421 ctggagcgcc acggtgccag cctcggcagt tagcgcattg ggtgaagcta actacacggt
     6481 aacggcaaac gtcaccgaca aggcaggcaa cagtaattcc gccagtcata atgtgctggt
     6541 caacagcgcc ttgcctgccg ttaccattaa cgcggtggcg actgacgata ttatcaatgc
     6601 cgacgagtcg gacaatgcgc aaaccatcag cggccaggtg acgggggccg cggcgggaga
     6661 taccgttacc gtgacgctgg gcggcaacac ctacaccgcc acggtgcagg ctaatttaag
     6721 ctggagtatc agcgttccgg cggcggatat tcaggcgctg ggtaacggcg acctgacaat
     6781 taatgccttg gtcaccaatg gcgtgggcaa caccggcagc ggctcgcgcg atatcacgat
     6841 cgacgccaat ctgcctggcc tgcgggtcga taccgtggcg ggcgatgatg tgattaacag
     6901 cattgagcac aatcaggcgc tggtgatcac cggcagtagc accgggctga cggcgggaac
     6961 ggcgttaacg gtcgagatta acaacgttac ttacgctgcc acggtgttag ccgacggcac
     7021 gtggagcctc ggtgttccgg cggtagacgt cagcaactgg ccagcgggta cggtgaatat
     7081 cacggtaagc ggcaccaaca gcgccggaac aacctccacc attacccatc cggtcaccgt
     7141 cgatctggcg gcggttgcca tcaccattaa caccctttcc ggcgatgatg tgatcaacgc
     7201 cgtcgaaaaa ggcgaaacgc tggtggtgag cggcagcacc agcggtgtgg aagccgggca
     7261 gacggtgacc gtcacctttg gtggcaaaaa ttacaccact accgtggaag ccaacggcag
     7321 ctggacggtg aatgtgccgc ctgccgatct cgctgcgctg ccggatggcg cgggcaacgt
     7381 gcaggcgagc gtcagcaaca tcaacggcaa taacgcccag gcggatcgcg cgtacagcgt
     7441 tgatgccacc gcgccacttg tgaccatcaa caccatcgcc agcgacgata ctcttaacgt
     7501 gagcgaagcg ggcgcgggga taaccatcag cggtaccacc acggcgcagg ccgggcagac
     7561 gctcaccgtc acgctcaata acaacacgta ccagaccacc gttcaggcgg atggcacctg
     7621 gagcgtgaat gttccggcag cggatttaag cggattaacc gccagcagtt acaccgtgac
     7681 cgccacggtg agcgacaaag cgggtaaccc ggcaagcgcc gaccacgcgc tggcggtgga
     7741 cgttaccgca ccggatctca ccattaatac cgtcgcgggc gatgacatta tcaacgccat
     7801 cgaacacggt caggcactgg tcgtcagcgg aaccagtacg ggcgcggcgg cgggcgatgt
     7861 ggtcacggtt aacctgaacg gtaaaaacta caccaccacg ctggacgcct ccggtaactg
     7921 gagcgtgggc attccggcgg cggatgtcac ggcgctggca accggcagcc agacgatcac
     7981 cgccagcctg agcgatcgcg cgggcaacag cgacagcact acccataatg tgaccgttga
     8041 tctcagcggc ccgacgctga ccatcagtat cgtctccggt gatgacatta ttaataatgc
     8101 ggagaaaacc caggatctaa tcatcagcgg cggctccagc ggcctggcga ccggcactac
     8161 ggtcaccgtg atgttgaacg gcctggcgta cagcgcaact accgacggtt ctggcaactg
     8221 gagcgttact gtgcctgctt cggcggtcgg cgcactgggc gaggcggtgt accagattag
     8281 cgccagcgct accgacagcg cgggcaacag cggcatcacc acccatacgg tgaatgttga
     8341 atcgctcctg ccaggggtga tcattaatac cgtggcgggc gatgacatta tcaacgccgc
     8401 tgaaattggt gttaaccaga ccatcagcgg acaggttacg gggacggcag cagccggaga
     8461 tacggtcact gtcacgctcg gcggtaatca atacgtcgcc accgtacagc cagatttaag
     8521 ctggagcgtc agcgtaccgg cgaacgtttt gcaggcgctg ggcaacggtg agttgaccat
     8581 cagcgcctcg gtgaccaatt ccgcaaataa tactggcacc acaacgcacg acatcgtgat
     8641 agatgccaac ctgccaggcc tgcgggtcga taccgtggca ggcgatgatg tgattaacag
     8701 catcgagcac actcaggcgt tggtgatcac cggtagcagt agcggactgg cggcgggcgc
     8761 ggcgttgacg gtggttatca atagcgtcac ctacggcgca acggttttag cggatggtag
     8821 ctggagcgtt ggtgttccgg cggcagatgt cacaaactgg cctgcgggga cggtcaatat
     8881 tgccgtctct ggcaccaaca ccgccggaac gacaaccagt attacccatc cggtgaccgt
     8941 cgatctcgca gccgtggcga tcaccatcaa ccccctttcc actgacgatg tgattaacgc
     9001 cgctgagaaa ggctctgatt tgcagctctc cggcaccacc tccgatgtgg aagcggggca
     9061 aaccatcacc gttatcttcg gcggcaaaag ctacaccacc acggttgccg cggataatac
     9121 ctgggggctg acaatcccag ccgccgatct cgccgcttta ccggatggcg cagcaaacgt
     9181 ccaggccagt gtcagcagcg tcgcaggtaa cagcgcccag gcaacgcatg tttacagcgt
     9241 tgatgccacc gcgccctccg tcaccatcaa caccatcgcc gcagacgata ttcttaacgc
     9301 cgctgaagcg ggatcggcgc tgatcatcag cggcaccagc acgacagaag ccgggcagac
     9361 ggtaaccgtg acgcttaacg gcgttaatta cagcggcaac gtgcaggctg acgggagctg
     9421 gagcgtcagc gtaccaactg gtgatttagc taaccttacc gccagctcgt ataccgttaa
     9481 cgcctcggtc agcgacaaag ccggaaatcc ggcttcggca acgcataatc tgacggtgga
     9541 ccttgccgct ccggtcgtca ccatcaacac ggtggcgggc gatgacatca tcaacgccac
     9601 ggaacacgga caggcgcaga tcatcagcgg ctcggcaacg ggcgcgacta ccggtaatac
     9661 ggtttccgtg acgattggca cgaccaccta taccaccgtg ctggacgcca acggcaactg
     9721 gagcatcggc gtgcctgcca gcgtgatttc cgcactggcg cagggcgatg tgaacattag
     9781 cgccacggtt accgactccg caggcaacag cggcacagcc tcgcacgttg tcagcgtggc
     9841 gctcggcgct ccggtgctcg ccattaacac cattgccgtc gatgacatca tcaacgccac
     9901 ggagaaaggc gcggatctgg cgatttccgg caccagcgac cagcctgcgg gcacgcagat
     9961 taccgttacg ctcaacgggc aaaattacac caccacagcc gatgcttccg ggaactggag
    10021 cgtgaccgtt ccggcgtctg cggttggtac gcttggtgaa gcgacttata ccgtgaccgc
    10081 ctccgccact gacgccgatg gcaacagcgg ttccgccagc cataacgtac aggttaatac
    10141 cgcgctgccg ggcgtcacca ttaacgtggt ggcaacggac gatattatta acgccgccga
    10201 agcgggcgtg gaccagacca tcagcgggca ggtgacgggt gcggcggcag gcgacacggt
    10261 gaccgtcacg ctcggcgggg cgacttacac tgccacggtg caggcgaatt taagctggag
    10321 cgtcgatgtt ccggcctccg cgttacaggc gttgggcaac ggtgagctga ccatttcggc
    10381 ttcggtaacg aacagcgtag gcaataccgg caacggcacg cgtgaaatca ccatcgacgc
    10441 gaatctgcca ggtctgcggg tcgataccgt ggcgggcgat gatgtggtca atatcatcga
    10501 gcacgggcag gcgctggtga ttaccggcag cagctccgat ctggcagcgg gcagcaacgt
    10561 cacgctgacc ataaacgggc aaacgtatgt tgcggcggtg ctggcggatg gctcatggag
    10621 cgtcggtgtt ccggcggcag atgtcagcgc ctggcctgcg gggacggtca cgattacagc
    10681 cagcggcaac accaccgccg gaaacccggt gagcgttacg catccggtga ctgtcgatct
    10741 cacggcggtg gcggtcagca tcaacgccat taccgtcgat gatgtgatta acgccgctga
    10801 aaaaggcgcg gcgttaacgc tctccggcag cacctctggc gtcgaagccg ggcaaacggt
    10861 caccgtgaac tttggcggaa aaacctacac tgcaagcgtg gcgacgaacg gttcctggag
    10921 caccacggtt ccggcggcag atatggcggc cctgcgtgat ggcgatgcca gcgcacaggc
    10981 cagcgtcagc aatgttaacg gcaacagcgc caccactact cacgcttaca gcgtcgatgc
    11041 cagcgcgcca acggtgacca ttaacaccat tgcgggcgac gacattctta acgccgccga
    11101 agccggagcg gcgctgacca tcactggcag cagcacggcg gaagcggggc agacggtgac
    11161 cgtcaccctc aatggcgaaa actatactgg cacggttcaa acggacggta gctggagcgt
    11221 cagtgtgccg caagccgacg taagcgccct gaccgccagc aactacaccg tgagcgcggc
    11281 ggtgaacgac aaagccggaa acccggcctc ggttaaccac aacctgacgg tggatacttc
    11341 tgttccggtc gtcaccatca acacggtggc aggcgatgat gtgatcaacg cgacggaaca
    11401 cgcccaggca cagatcatca gcggatccgc cacgggtgcg gcaaccggta gcaccgtgac
    11461 ggtgactatc ggcacaaata ctttcaccac ggtgctggat gccagcggca actggagcgt
    11521 cggcgttccg gcaagcgtcg tctcggcact ggcgaacggc acggtgacca ttaacgccag
    11581 cgtcaccgat gtcgccggta acagcggcag cgccacgcat caggtgacgg tcaataccgg
    11641 gctgccgacc attactttta acgccatcag cggcgataac gtcctgaacg ctgatgaaaa
    11701 aggccagccg ttgaccatca gcggcggcag tacggggctg gcgacgggcg cgcaggtcac
    11761 cgtcacgctc aacggtcaca actacagcgc caccaccgac gcgtcgggca actggacctt
    11821 aaccgtgccg gtgagcgatc tggcggcatt aggtcaggcc aactatacgg tcagcgccag
    11881 cgccaccagt gcagcaggca acaccgccag cagccaggcg aatttactgg tcgacagcgg
    11941 cctgccggac gtcaccatca acaccgtggc aggcgacgat attatcaacg ccgccgaagc
    12001 gggggccgat caaaccatca gcggggtggt gactcgcgcc gccgctggcg atacggtcac
    12061 cgtgacgctg ggcgggaaca cttacaccgc tacggtacag agcaacttaa gctggagcgt
    12121 cagcgttccg acagccgatc tccaggcgtt gggcaatggt gatttgacca ttaccgcctc
    12181 ggtcaccaac gctaatggca acaccgggag cggcacgcgg gatatcacca ttgatgccaa
    12241 cctgccgggg ctgcgcgtag ataccgtggc gggcgatgat atcgtcaaca gcatcgagca
    12301 cgggcaggcg ctggtgatca ccggcggcag tagcggcctg aatgcaggtg ctgtgctgac
    12361 ggttaccatc aacagtgtgg cgtattccgc cacggtgcag gcggacggaa gctggagcgt
    12421 tggcattccg gcggcaaacg tcagcgcctg gcctgcgggg ccgttaaccg ttgaggtagc
    12481 cgggcaaagc agcgccgata acccagtcag cgtcagccat ccgttcaccg tcgatttaac
    12541 ggcggtggca atcagcatca atacggtcgc cagcgacgac gtgattaacg ccgccgagaa
    12601 aggcaccaat ctgacccttt ccggcagcac cagcgggatt gaaagcgggc aaaccgtaac
    12661 cgtcaccttt ggcggcaaaa cctacaccgc aagcgtcgcc gccaacggaa gctggagcaa
    12721 aacggttccg gtggcagatc tggcaactct gccagacggc gcggcgaacg tgcaggccag
    12781 cgttagcagc gcgagcggta acagtgcctc ggcgacccat gcgtatagcg ttgacgccag
    12841 cgcgccgacg ctcaccatta acaccatcgc cagcgacgat atccttaacg ccacggaggc
    12901 cggaagcccg ctcaccatca gcggctccag caccgccgaa accgggcaga cggtcaccgt
    12961 cacccttaac ggcgcaacct acaccggcac tgtacaggcg gacggcggct ggagcgtcag
    13021 cgttcctact tcagccctgg gcgcgctcaa cgcaagcaat tacaccgtca gcgccacggt
    13081 caacgacaaa gcgggcaacc ccggcagcgc cagccacaat ctggcggtag ataccaccgc
    13141 gccggttctc accattaaca ccgtggcggg cgatgacatc atcaacgacg ccgaacacgc
    13201 ccaggcgctg gtgatctccg gtaccagcac gggcggggaa gcgggcgatg tggtgagcgt
    13261 tgtgctcaat ggcaaaacct acaccaccac gctggatgcc tccggtaact ggagcgtcgg
    13321 cgtaccggcg gcggatgttg cggcgctggg tagcggcgca cagaccatca ccgccagcgt
    13381 cagcgatcgg gcaggcaaca gcgacgacgc cagccgcacc gtgaccgtca gcctcagcgc
    13441 gccagtgatt agcatcaaca ccatcgcggg cgatgatgtg atcaacgcga cggaaaaagg
    13501 atctgatctg gcgctttctg gcaccagcga tcagcctgcg ggtacggcga tcaccgtcac
    13561 cctgaacgga caaaactaca gcgccaccac ggatgcctcc ggcaactgga gtgttaccgt
    13621 gcctgcctcg gcggtcagtg cgctgggcga agcgacctac agcgtgacgg cgagcgtcac
    13681 caatgctcag ggtaacagca gcaccgccag ccataacgtg caggttaata ccgcgctgcc
    13741 gggcatcacc attaatccgg tggcgacgga cgatattatc aacgcttcag aggcaggcag
    13801 cgcgcaaact atcagcggcc aggtaaccgg tgcggcggcg ggcagcaccg tcaccgttga
    13861 actgggcggt aaaacttaca ccgccaccgt gcagccagat ttaagctgga atgtaagcgt
    13921 acctgccgcc gactggcagg cgctgggtaa cggtgagtta acggttaatg cctcggtgac
    13981 caacgccgtt ggcaataccg gcagcggcac gcgcgatatc accatcgatg ccagtctgcc
    14041 cggcctgcgg gtggataccg tcgcgggaga tgatgtggtc aatatcatcg aacacagtca
    14101 ggcgcaggtg atcaccggca gcagctccgg ctttgccgca ggcacggcgt tgacggtggt
    14161 gattaacaac caaacttacg ccgccacggt gctggtgaat ggtacctgga gcgtcggcgt
    14221 tccggcggcg gatgtcagta actggcctgc gggaacgctg aatattaccg ttagcggcgc
    14281 gaacagtgcc ggaacgcaaa ccagcattac ccatccgctg actgttgatc tcactaccgt
    14341 tgccatcagc attaacgcta ttacccctga tgatgtgatt aacgccgccg aaaaaggcgc
    14401 ggcattaacg ctctccggca gcacgtccgg cgtcgaagcg gggcaagccg tcaccgtcac
    14461 ctttggcggt aaaacctaca ccaccacggt ggcggcaaac ggtagctgga gcaccactgt
    14521 tccggctgca gatttggcgg cgctgcgtga cggcgatgcc agcgcccagg tgcgggtgac
    14581 taacgtcaac ggcaacagcg ccacagccac gcatgaatac agcgtcgata gcgccgcgcc
    14641 aactgtgacc atcaacacca ttgccagcga taacatcatc aacgccagcg aagcggcggc
    14701 gggcgtaacg gtgtccggca ccagcacggc gcaaaccggg cagacgctca ccgtcacgct
    14761 taacggcact aactaccaga ccacggtgca ggcagacggc agttggagct taacgctgcc
    14821 cgccagcgac ttaaccgcac tggccaataa cggctacacc ctgaccgcta cggtcagcga
    14881 tctggcgggt aaccctggca gcgccagcaa aggtgtgacc gtcgatacca ccgcgccggt
    14941 gatcagcttt aacaccgtgg cgggcgatga tgtgattaac aacgtcgaac acactcaggc
    15001 gcagattatc agcggcaccg ccacgggcgc ggtcgcaggc gaccgcctgg tggtgaccat
    15061 cgccgggcag cagtatgtca ccagcaccga tgccagcggc aactggagcg tcggcgtgcc
    15121 tgccagcgtg atttccggcc ttgcggacgg cacggtgacc atcagcgcca ccattaccga
    15181 tagcgcaggc aacagcagca cgcagacgca caacgtacag gtgaacacgg cggcagtgtc
    15241 gctttcggtc agcaccatca gcggcgataa tattattaac gccgccgaag cgggcagtgc
    15301 gctgaccctg agcggcactg gcactaattt cgcgacaggt acagtggtga ccgtgttgct
    15361 taacggcaaa ggctacagcg ccaccattca gagcaacggg agctggagcg tgaacgtgcc
    15421 tgcggcggat gttgcggcgc tcagtgatgg caccagctac acggttagcg cctccgctca
    15481 ggacagtgcc ggaaacagcg ccacggcttc gcgcagcgtg gcggtggatc tcaccgcgcc
    15541 ggtgatcagc attaacaccg tttcgacgga tgaccgactc aacgccgccg aacaacagca
    15601 gccgttaacc cttaacggct cgaccagcgc ggaagtggga cagacggtca ccgtcacctt
    15661 tggcggcaaa acctataccg ccacggttgc tgccaatggc acctgggcgc tgaacgtgcc
    15721 tgcggcagat ctggccgcgc tcgggcaggg cgcgcagacc attaccgcca gcgtgaacga
    15781 tcgcgccggt aaccccggac aggccacgca cgccctgacc gtcgataccg ttgcgccaac
    15841 ggtcaccatc gccacggtgg cgggtgacga tattatcaac aacgccgagc agcttgccgg
    15901 gcagaccatc agcggcacca ccaccgccga agtaggccag acggtgaccg tcacttttaa
    15961 tggtcagagc tggagcgcaa cggtcggcag cggcggaagc tggtcggtat ttattccggc
    16021 gcagcagttt gccggattaa gcgacggcag ctacaccatc agcgcgacgg ttagcgatca
    16081 ggccggaaac cccggcagcg ccagccgtgg cgtgacgctg aacggcgacg tgcccaccgt
    16141 caccatcaac acctttgcgg gcgatgatgt ggtgaatgca gcagaacacg gctcgtcgct
    16201 ggtgatcagc ggcaccacca ccgcgccggt cggacagacg ctgaccctga ccttaaacgg
    16261 caaaacctac accaccacgg tacagaccgg cggtagctgg agctacaccc taggcagcgc
    16321 cgatgttacc gcgctggcgg acggcaacgc ctacgtgatt aacgcctcgg tgagcaatgc
    16381 cattggcaac accggtagca gtaatcacac cattaccgtc gatctcagcg ctccggcgat
    16441 gggcattaat atcgattccc tgcaagccga cactggcctt agcgccagcg actttatcac
    16501 cagcgtcagt ccggtagtgg tcaacggctc gctcaccgcc gcgcttgcca gtaacgagac
    16561 ggcgcaaata agtatcgacg gcggcacaac ctggaccacg cttaccgtta ccggcacgac
    16621 ctggcgctat aacgacagcc gcacgctgac cgatggcaac tatctctatc aggtgcgggt
    16681 gattgacgca gcgggcaacg ttggcgcgac cgacagccag aatgtggtga tcgacactac
    16741 cgcgccagat cccgcggtga aaaccatcgc catcagcgcg atcaccaccg atacgggctt
    16801 gatcactaac gattttgtca ccagcgacac gacgcttgcg gtgagcggca cgctgggggc
    16861 gacgctttct gccggtgagt tcgcgcaaat cagcattgat ggcggcacca cctggcagaa
    16921 tctttcggta agcggtttga cctggagcta tcttgacggg cgtacgctga ccgatggcaa
    16981 ctacaactac caggtgcggg tgatcgacac cgccggaaat atcggcgcga ccgccagcca
    17041 gatagtcact gtggatacca ccgcgccgct ggcgagcaaa accatcgcca ttgcgagcat
    17101 cagcgatgac accgggttaa gcagcagcga ttttgttaca cgcgacacca cgctgaccgt
    17161 gcgcggcacg ctcggcgcgg cgcttgccgc cgatgaacgg gcgcaaatca gcctcgacgg
    17221 cggcgtcacc tggactacgt taaccgttat cggcaccagc tggagctatg ccgatggtcg
    17281 cacgcttact gacggcacct ggaactacac ggtgcgggtg gtggatctgg cggggaacgt
    17341 tgggcagacc gcaacgcaaa acgtggtggt cgataccacc agcccggaag cggcgaaaag
    17401 tatcaccatt accggcatca gcgatgacac cggaaccagc agcagcgatt ttattaccag
    17461 cgacaccacg cttaccgtgc gcggcgtgtt gggcgcggcg ctcggcgcta atgagttcgc
    17521 acaaatcagt accgacaacg gcgcaacctg ggtgaacgtg acgctagcag cagacggcct
    17581 gaactggagt tacgttgacg gacgaaccct caccaacggc accaccacct ggcaggtgcg
    17641 ggtggtcgat ctggcgggca acgttggcgc aaccggcagc cagtcggcgc agattgacac
    17701 cgttaacccg gcgcaggtgt tgaccatcgc cagcatcagc accgacacgg ggagttcggc
    17761 aactgacttt atcaccagcg atacctcgct cacgctaacc ggttcgctgg gagcggggct
    17821 tgccagcggc gaagtggcgc agattagcct tgatggcggc gcgacctgga ctacgctcac
    17881 caccaacggc acgcagtgga cctacactga cagccgcacg ctgaccgacg gcagctatgt
    17941 ttatcaggtg cgggtgctgg atctggccgg gaacaccggt ccggtggtgt cgaaaacggt
    18001 ggtggtcgat acgattaacc ccaccgccac gccaggtatt gtgtcgtata ccgatgatgt
    18061 cgggcagcgg caggggacgt taagcagttc gcaggccacc gacgacacta cgccgctgct
    18121 gaacggtgta ctttccgcgc cgcttgccag cggtgaagtg gtctatctct accgtaacgg
    18181 cctgctgtta ggtgcggtaa cgatggtcgg cgcactgaac tggacctaca gcgacagcgg
    18241 gctggtcagc ggtgcctata cctacagcgc gcgagtggtg gatttggcgg ggaatatcac
    18301 ctcctccagt gattttgtcc tgacggtcga tacctctatt ccgaccacgc tggcgcaaat
    18361 caccagccag accacgcgcg atactacgcc gattatcagc ggggtgatca ccgccgcgct
    18421 tgccagtggg cagtatgttg aagtggtgat caacggcaaa acctacacct ctgaaccggg
    18481 cggcgcggta gtggtcgatc cggcgcacaa cacctggtat gtacagttgc cggataccga
    18541 tgcgctgaca gtttccgcga ccgcctatac cgttactgca caggtaaaaa gttcggcggg
    18601 taacggcaat aacgccaata ttagcaacgg cacggtgacg gttaacgcgg cgattgatta
    18661 cacaccgacc tggaccaccg ccagcaaaac caccgcctgg gggctgacct acggcctcga
    18721 ctcgcacggg atgtggacgg tgctggcaaa ccagcaggta atgcaatcga ctgacccact
    18781 cacctggtcg aagaccgcgc tgacgctgta tcagagcggc aacaactacg ccaccagctc
    18841 catcgccgat tacgaccgta acggcacggg cgatctgttt atcacccgtg atgactacgg
    18901 tacaggctat atcaacggct ttaccaataa cggcgacggc accttttcca gcgctattca
    18961 ggtcaccgtc ggcaccctga cgtggtacgg ctcgattgtg gcatttgata aagagggcga
    19021 cggctatctc gacttctgga ttggcgacgc gggcgggccg gactccaaca cctttctgtg
    19081 gaacaacgca ggcacgctgg taggcaactc caccacgtcg aacagcggcg gtagcgccac
    19141 ggtgggcggg gcggtgacgg ggtatctttc gctcaacgaa ggttctggcg tcgatctgaa
    19201 caatgacggc aggatcgacc tggttcagca cacctataac ctgaacaact attacacgct
    19261 gtcttcgctc atcaaccagg ggaatgggac gtttgtctgg gggcagaaca ccaccaatac
    19321 cttcctgagc ggggcgggta gtggcgctat gagcagcagc gtttccatga cctgggccga
    19381 tttcgatggt gacggcgata tggatctctt cctgcccgcc agccagggaa gagctaacta
    19441 cggctcgctg ttattcaaca ccaacggcgt actgggtagc ccggtggcgg tgggcgcaac
    19501 ggcaaccacc tacgccagcc agtttagcct ggcggtggac tggaaccatg acggcctgat
    19561 ggatatcgcc cgtatcgccc agaccggaca atcgtatctt tacaccaacg ttggtggtgc
    19621 cagcaactgg acacaatcag ccctcggcgg cagccagagc ggtaccacca gcggcgtggc
    19681 ggcaatggac tacgactggg acggcgcggt ggatgtgctg gtgaccaaac agtcgggcag
    19741 cgtgttcctg atccgcaaca ccaacacggt gagctacggc acttcgctac acctgcgcat
    19801 caccgatccc aacggcatta acgtctatta cggcaatacc gtgaagctgt acaactcggc
    19861 gggagtgctg gtcgccacgc aaatcatcaa cccgcagtcg ggtatggggg ttaacgacac
    19921 ctcggcgctg gtcaacttct acgggctgaa tgccggagaa acctacaacg cggtgctgat
    19981 caaatccacc ggcaccaccg ccagcaatat cgaccagacg gtcaacacca gctggggcgg
    20041 tttacaggcc accgatgcca ctcacgctta cgacctcagc gccgaagcgg gtaccgcgag
    20101 caacaacggc aagttcgtcg gcaccggtta taacgacacc ttctttgcca ccgcaggcac
    20161 cgatacctac gacggttccg gcggctgggt gtacagctcc ggcaccggaa cgtggctggc
    20221 gaacggcggg atggacgtgg ttgatttccg gctttcgacg gtgggcgtga cggctaactt
    20281 aagcagcacc gccgcgcagg ccaccggttt taacacctcg acatttacca atatcgaagg
    20341 catttccgga tcgaatttta acgacactct gaccggcagc agcggcgata accaactgga
    20401 agggcgcggc ggcaacgaca cgctcaacat cggcaacggc ggccacgaca ccttgctcta
    20461 taaactgctc aacgccagcg acgccactgg cggcaacggt tcggatgtgg tgaatggctt
    20521 tacggtaggc acctgggaag gcactgccga caccgatcgc attgatatcc gcgagttgtt
    20581 gcagggcagc ggctacaccg gaaacggcaa agccagctat gtcaacggcg tggcgacgct
    20641 ggatgcgcag gccggaaaca tcggtgactt tgtcaaagtc acccagagcg gcagcgacac
    20701 catcgtgcag atcgaccgcg acggcacggg cggcactttt gcgacaacta acgtggtcac
    20761 gctgacgggc gtgcacaccg acctcgccac tttgctggcg aatcatcagt tgatggtggt
    20821 gtagataatg aaaaaaaatg cccagtccgt ggaagcctgg ctggaggcga tgatcgccgt
    20881 ggcgcgctat tatcggctcg atttttctca ggagaatgtg cgcgcgaccg tcaactggga
    20941 gcgcgacagt aagcgggaag agttgctcac cgacatggcg cggcagttgg gaatggggtt
    21001 acggctggtg gagttttccg ccgattcgct taacccctgg cgtctgccgc tgatcgcggt
    21061 gtttgataat cagcagattg gcgtgatcac ccgtcgcgac aatcatgaca atatcagcgt
    21121 tcagttcagc ggtgacgaag ggctggaaac cacgcttaac gtcgcggatg tcgaggataa
    21181 aatcgttgaa ctggcgctac tgcgcccgct cagcgccatc cctgacgcgc gggtagatga
    21241 ctatattcgc ccgtatcagg cgaactggtt ctggagtctt tcgctaaaag actggcggcg
    21301 ctacggcgat attatgctgg cgtcactggt ggcgaacgtg ctggcgctgg cggcgatgat
    21361 tttctcgatg caggtttacg accgcgtcgt tccggcgcag tcctacccga cgctatgggt
    21421 gctgtttgcc ggggtgatga tggcgatcct gtttgagttc tgtatgcgca tggtgcgtac
    21481 gcatctctcg gatgtgatcg gcaagcgggc ggatctgcgt atttcggatc gcgtttttgg
    21541 tcacgcatta cggctgaaaa acaacgtgcg atcgaaatcc accggctcgt ttatctcgca
    21601 gatccgcgaa ctggaatcgg tgcgggagct gattacttcc accactatcg gcgcggtggc
    21661 ggatctgccg ttcttcctgc tgtttgtttt tattctgtgg atgattggcg gctggctggt
    21721 gctggttgtt ttgctggcgc tgccgttatt ggtgatcccc ggcctgctgg tgcaacgccc
    21781 gctggcgcgg ctggcgaacg aaggaatgcg cgagtcagcg gtacgcaacg ctacgctggt
    21841 ggaagccgtg cagtcgattg aagatattaa actgcttcgc gctgaacagc gttttcagaa
    21901 tcagtggaat cacaccaacg acgtggcctc gtccatcagc atgaagcaac gttttctcac
    21961 cgggctactg ctcacctgga cccaggaagt gcaatccatc gtctacgtcg tggtgctgtt
    22021 ggtgggctgc tttatggtga tgaacggcga catgaccact ggcgcactgg tcgggacgac
    22081 gatcctggct tcgcgcacca tcgcgccgct gtcgcagata tccggcgtgc tctcgcgctg
    22141 gcagcaggcg aaagtagcac gtaacggcct tgatgaactg atgaagcgcc ccgtggatca
    22201 gccggaacat ggcaaactgg tgcataaagc ggtgctgcac ggtaattatc agttcagcaa
    22261 cgcggtgttt tattacgacg aagaagagaa aattgccgat gtggcgatcg gtaaactcaa
    22321 cattcaggcc ggagagaaaa tcgccattct cgggcgcaac ggcgcgggta aatcgacgct
    22381 actgcaaatg ctcgccggaa tgcgtatcgc ccagcagggg caggtgttgc tggataacat
    22441 cagtatcggg cagcttgatc cggcagatct gcggcgagac atggggctac tcagccagac
    22501 cgggcgactg ttctttggtt cgctgcggga aaacctcacc atggggatgc cggaagccag
    22561 cgatgaagat atcgaacgcg cgttaaccct cagcggagcg ctgccctttg tgcagaagca
    22621 gaaaaacggc ctcaactaca tgattcagga agggggattt ggtctttctg gcggtcagcg
    22681 acaaacgctg ctgctggcgc ggctactcat ctctcaaccc aatatcgtcc tgcttgatga
    22741 acccagcgcc tcgctggatg aaatggcgga agcgtattta atcgagcaac tgaaacagtg
    22801 gattgggcat cgcactctga tcgtcgctac ccaccgcacg gcgatgctgc aactggtgga
    22861 taggataatc gtgatggatc aggggcggat tgtgatggac ggcgcgaaag aggccatttt
    22921 gcgtgaacag ggcgaaccca cggcgcgacg ggtggtgttg caggaaaaga ataaggggtc
    22981 tgccgcatga acggacaact gcaatataac cgtggcctga ctgaaccccg cttgccgcgt
    23041 tcggcgctgg cggtacgcgt taccgctgtg atgctgctct gctttcttgg ctgggcgtgg
    23101 tacttccagc ttgatgaagt caccaccggc agcggcacgg tagaaccctc cgggcgcgag
    23161 caggtggtgc agtcgctgga aggcggaatt ttgtatcatc tcgacgtcaa ggttggcgat
    23221 attgttgaac agggccagcc gctggcgcag cttaaccgca ccaaaacgga atccgacgtc
    23281 caggaagcga tgtcgcggct gtacgctgcg ctggcaacct cggcgcggct gcgggcggaa
    23341 gtgagcaata aaccgctggt cttccctgac gaactgaata aattccccga acttatcgag
    23401 tcggaaacgg cgttgtacaa cactcgccgc gacgggttga acaaagccac caccgggctg
    23461 acgcagggga tcagtctggt caatcgcgaa ctggcgatga cccagccgct ggtaaaacag
    23521 ggggcagcga gcagcgtcga agtactgcgc ctgcaacgcc aggccaatga actggaaaac
    23581 aaactcagcg acgtgcgcac gcaatactac gttcaggcgc gggaagagtt agcgaaagcc
    23641 aatgcggaag tggaaaccca gcgttcggtg atccgtgggc gtgaagactc cctcaccagg
    23701 ctcaacttta ctgcgccagt tcgtggcatt gttcaggata ttgacgtcac cacggttggc
    23761 ggcgtcatcg cccccggtgg taaactgatg accatcgtgc cgctggatga gcaactgctg
    23821 attgaggcaa aaatctcccc gcgcgatgtg gcgtttatcc atccggggca aaaatcgctg
    23881 gtgaaaataa ctgcctacga ttactccatc tacggcggtc tgccgggaga agtagcggtg
    23941 atatcaccag acaccgtgca ggatgaagtg cggcgcgatg tttattacta tcgggtgtac
    24001 attcgcactt tcagcaacca tctggaaaac aaaagcaaac agcagtttcc gatattcccc
    24061 ggtatggtgg cgacggtcga tatccgcacc ggcaaaaaaa gcgtgctgga ttatctgttg
    24121 aaacccttta acaaagcaca ggaggcgttg cgcgaacgat gaacattagc gatgtagcaa
    24181 aaattaccgg cctgaccagc aaagccattc gcttctatga agagaagggg ctggtgacgc
    24241 cgccgatgcg cagcgaaaat ggttatcgaa cctacacgca gcagcatctc aacgaattga
    24301 ccttactgcg ccaggcgcgc caggtgggct ttaacctgga agagagcggc gagctggtga
    24361 atctgtttaa cgacccgcag cgtcacagtg ccgacgtcaa acgtcgcacg ctggagaagg
    24421 tggcggagat tgaacggcat atcgaagaac tgcaatctat gcgcaaccag ctactggcgc
    24481 tggcgaatgc ctgccccggt gatgacagcg ccgactgccc gattatcgaa aatctctccg
    24541 gctgctgtca tcatcgggcg gggtgaaaat cagttaattc ccgtttgcac cgaaagaata
    24601 tgctgcaaac tttctaccat aatgcgcccc gactgaaata agccttcctg gatttcgttg
    24661 ttaacattca cagcgtgaac cagggaggct aataatacta cttgcaagaa ggcaaaaata
    24721 aacgcaccga taatcataat atgcgtcagg ggtattttcc tttgctcctt cattttaaat
    24781 aacccacaaa ctggccttct ttcattaagg cgacttttcc tgtgtgatga taaacaatga
    24841 cgttatacag acctgactga taaacagtgg catctttatt gtcttcatca attgcacata
    24901 atgaatcatt gagataagca aagtttttac tgctcattct aaaggtcatg ccaaatcctt
    24961 tataaatggc tttctttgcc gcctgggcat gttgtgcggc ttctgcattt ttaaatgcct
    25021 gcgcttcgga agcacttaaa atagccgtct ggcgctgctg ttctgcgaga taacaggtgt
    25081 catggctgac gccctgcgcc tggcaatctg cgatgcggga agcgggtgag ctacagccag
    25141 ccagtaatat aaccgccagc agggaaaacg atttaatgga gctattcatc attgttaacc
    25201 ttaaataaac tgcggaatat tgctataccc gtcatacttc aagttgctgc aactcgaatt
    25261 atttagagta taataagaaa ttgctattaa aattagaacg aataccccac gccgacaccc
    25321 gcgccgaaat cagattgtgt atccgcagag actcccgctt taattaccgt atgaattcca
    25381 gcattaaacg aagcaccgac agataccgca gattcattgt tgaatgtacc cgccccggca
    25441 gacaccatca taaaatcacc tgtcttaact tgaggctgtg aagcaatagc gacggctgac
    25501 gcaatacccg cattagcctc tttacggtta tcttccactt cgtgtttcaa cgaactgaag
    25561 ttgttattga gcgaattgat ttgctggctg ttatctgcaa cacgtttttg cgtgtttgtt
    25621 aactgctggt gcgtttgggc agaatcttgt gtcagacttt cgatttgctc atgtgcctgg
    25681 gccgagtctt gcgcaaggtt tcgaatttgt tcaccgtatt gcacgctatc gatttgctgt
    25741 tgcgtcgcga cattggaagc cagttcatta attctctgct cctgatcagc ggttgaacgt
    25801 tcattggctt cattttgcgc cgtcagtttg gcattaatca tcttttgata atgcgcagtt
    25861 gactggggat cgccgattgc agcgaccgtt ttttctgtgg ctgcgatctg cttctgttgc
    25921 tctgtaatct gcgtttgttg ggcttcagac tgtgattgtt gcgcatcagc agtggctgtc
    25981 tgaagagcgt taatcatgct ctgataacga gcagcggtct ttacatcacc cgtggcggca
    26041 acggtttttt gcgtggcagt gatttgttcc tgctgttcgg taatcgccgt actgtttttt
    26101 tcaatctggt ttttgttgtc gctgacgaca ccggaaagtt tctggccatt catcagcgcg
    26161 gtattagcat tcgcgttggc atgatcggca cttgctttaa catctgcgac atcatctttc
    26221 attgcactca catcggtgcg gacagcggca acatcttttt gtgtgtcgga aatggccgac
    26281 ttattggcgt cgatatccgt ttgcgccacc ataatggcat ggtgcgcgtc atcggcttta
    26341 agcgaagccc agttagcaga gtgctgggca tcctgaattg acgagtcctg tgcggcatcg
    26401 cgatgtattt ggttctgctc acttaacgcc atctgttcct gctgttcagc aatagaggag
    26461 ctgttttttt caatctggtt tttgttgtcg ctcacggcac cggagagttt caggccattc
    26521 atcagggcgg tattggcgtt tgtgttggca tgagcggcgc ttgctttaac atctgcgaca
    26581 tctttctgag tatcagcaat agctcccttg ttcgcatcaa ttgcagattg cgcagtggta
    26641 agcgcttttt tggcgtcatc tgcatttgcc gccgcaatgc ttgcgatatt gtgtgcgttc
    26701 tgaattgcgg tatcttgcgc cgcgtcgcga tcagcctgtt tttggtcggt gatgttgctg
    26761 tcagtctgta cggctgtact ggcagggtaa atattatcgc ctgtctgtgg gtccatatat
    26821 ccatgagaaa acaggtaatc ttgcgcggct tctgtattac ctgtccgaat catgaagcga
    26881 ttgatttttt gatcgatttc atcatgagac atacctgggt gaatcgctgt cagttctgtc
    26941 gctgccgctt caaccgtatc atctgcaaaa gcagatgggc tcacaatggt agcaacaacg
    27001 actgccagta aagacaactt tacagatttc atttattttt tctcgatcaa attaagttta
    27061 ttttggtttc taatagaaac taataatatt tctattggag ttaatttgcg ttgcaattta
    27121 atcgaaatct acgccgccat ttgtgtgatg taaagcgcgt ttttgtcttg tgtaagcgta
    27181 aacactgtct ataagaaact gaattggttt cttaagt
//
LOCUS       DAZGBC010000056        26952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGBC010000056 DAZGBC010000000
VERSION     DAZGBC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.056"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(190..516)
                     /locus_tag="ACHK1J_002286"
     CDS             complement(190..516)
                     /locus_tag="ACHK1J_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7911541.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(513..776)
                     /locus_tag="ACHK1J_002287"
     CDS             complement(513..776)
                     /locus_tag="ACHK1J_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO7911542.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(848..1714)
                     /locus_tag="ACHK1J_002288"
     CDS             complement(848..1714)
                     /locus_tag="ACHK1J_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7911543.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAIKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKENYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            complement(1799..1996)
                     /locus_tag="ACHK1J_002289"
     CDS             complement(1799..1996)
                     /locus_tag="ACHK1J_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7911544.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(2008..2496)
                     /locus_tag="ACHK1J_002290"
     CDS             complement(2008..2496)
                     /locus_tag="ACHK1J_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7911545.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(2493..2870)
                     /locus_tag="ACHK1J_002291"
     CDS             complement(2493..2870)
                     /locus_tag="ACHK1J_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7911546.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     NERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            complement(2960..3328)
                     /gene="cbeA"
                     /locus_tag="ACHK1J_002292"
     CDS             complement(2960..3328)
                     /gene="cbeA"
                     /locus_tag="ACHK1J_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7911547.1"
                     /translation="MSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYIAIYPTPGTKT"
     gene            complement(3378..4022)
                     /locus_tag="ACHK1J_002293"
     CDS             complement(3378..4022)
                     /locus_tag="ACHK1J_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO7911548.1"
                     /translation="MSRIITSTVYTLNELSATAREKARDGYRQHHADSNWYENVYEDF
                     RKVCDIFGIDLRQRVFQLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARIIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYSMDITVTRNSPTGQIMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDATVDEALLINEYTFTEAGLRAG"
     gene            complement(4041..4262)
                     /gene="yeeT"
                     /locus_tag="ACHK1J_002294"
     CDS             complement(4041..4262)
                     /gene="yeeT"
                     /locus_tag="ACHK1J_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7911549.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4325..4801)
                     /gene="yeeS"
                     /locus_tag="ACHK1J_002295"
     CDS             complement(4325..4801)
                     /gene="yeeS"
                     /locus_tag="ACHK1J_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7911550.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(4817..5302)
                     /locus_tag="ACHK1J_002296"
     CDS             complement(4817..5302)
                     /locus_tag="ACHK1J_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7911551.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVASLVPDEQRISFWPQHF
                     GLIPQWVTLESRVFGWMDRLYEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(5357..6175)
                     /locus_tag="ACHK1J_002297"
     CDS             complement(5357..6175)
                     /locus_tag="ACHK1J_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7911552.1"
                     /translation="MRLASRFGYANQIRRDHPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(6275..6508)
                     /locus_tag="ACHK1J_002298"
     CDS             complement(6275..6508)
                     /locus_tag="ACHK1J_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO7911553.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(6587..7042)
                     /locus_tag="ACHK1J_002299"
     CDS             complement(6587..7042)
                     /locus_tag="ACHK1J_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO7911554.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            complement(7118..9634)
                     /locus_tag="ACHK1J_002300"
     CDS             complement(7118..9634)
                     /locus_tag="ACHK1J_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911555.1"
                     /translation="MLQIVGALILLITGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     IFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            complement(9755..12877)
                     /gene="agn43"
                     /locus_tag="ACHK1J_002301"
                     /gene_synonym="flu"
     CDS             complement(9755..12877)
                     /gene="agn43"
                     /locus_tag="ACHK1J_002301"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO7911556.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNVGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNNTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQSN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDSSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGRGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGY
                     NGQATLNVTF"
     gene            complement(13250..14122)
                     /locus_tag="ACHK1J_002302"
     CDS             complement(13250..14122)
                     /locus_tag="ACHK1J_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7911557.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHTGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            15352..16836
                     /locus_tag="ACHK1J_002303"
     CDS             15352..16836
                     /locus_tag="ACHK1J_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO7911558.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PNESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(17158..17760)
                     /locus_tag="ACHK1J_002304"
     CDS             complement(17158..17760)
                     /locus_tag="ACHK1J_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911559.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLMYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(17847..18053)
                     /locus_tag="ACHK1J_002305"
     CDS             complement(17847..18053)
                     /locus_tag="ACHK1J_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7911560.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            18812..18961
                     /locus_tag="ACHK1J_002306"
     CDS             18812..18961
                     /locus_tag="ACHK1J_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO7911561.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
     gene            complement(19376..19576)
                     /locus_tag="ACHK1J_002307"
     CDS             complement(19376..19576)
                     /locus_tag="ACHK1J_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911562.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            19781..20350
                     /locus_tag="ACHK1J_002308"
     CDS             19781..20350
                     /locus_tag="ACHK1J_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7911563.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGIIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            20611..20988
                     /locus_tag="ACHK1J_002309"
                     /pseudo
     CDS             20611..20988
                     /locus_tag="ACHK1J_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            20992..21417
                     /locus_tag="ACHK1J_002310"
     CDS             20992..21417
                     /locus_tag="ACHK1J_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7911564.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLSVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            21664..21861
                     /locus_tag="ACHK1J_002311"
     CDS             21664..21861
                     /locus_tag="ACHK1J_002311"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7911565.1"
                     /translation="MPQDDFCIVNPGGCIVHFPANGRDIQDINAPDFPEQYQRLLARL
                     DYLTKPDAKASGQRNSGYSGF"
     gene            21889..22455
                     /locus_tag="ACHK1J_002312"
     CDS             21889..22455
                     /locus_tag="ACHK1J_002312"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7911566.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESYGAIERHLGCSDF"
     gene            22511..22775
                     /locus_tag="ACHK1J_002313"
                     /pseudo
     CDS             22511..22775
                     /locus_tag="ACHK1J_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22841..23038
                     /locus_tag="ACHK1J_002314"
     CDS             22841..23038
                     /locus_tag="ACHK1J_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7911567.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            23767..24891
                     /locus_tag="ACHK1J_002315"
     CDS             23767..24891
                     /locus_tag="ACHK1J_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO7911568.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIHFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(25033..26307)
                     /locus_tag="ACHK1J_002316"
     CDS             complement(25033..26307)
                     /locus_tag="ACHK1J_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO7911569.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
BASE COUNT         6466 a         6522 c         6889 g         7075 t
ORIGIN      
        1 ggccaccaat gaggccccca tgtgcaattc gaattctttt gacgctataa tccctctttt
       61 ttgtgctttg ctgtaatcat tttgtctggt tcgatatgct gaattgcgga atcgaagtaa
      121 caacaaaata cagagattta taaaatacct ggctacctgt gttagggagc gttaacgcac
      181 agattttgtt tatggctgcc agaaatgatc tgatggtttg tcgtctggct ctgcaagtac
      241 cctgttgata tcccgggaac catgaacgat acgttcgata cgaacttcag tatccagtac
      301 cctgaacagc agaaggtaac gaccatacga acaacctctg atcccagccc ctaattcggg
      361 tctttctctg tagagtactg gcgattcggc tatcaacaga cattgttggt aaagctcttc
      421 ggtaaaactg attgcacgga caggattatc ctgagcgatc cagtcaccta tagcttcaag
      481 atcgtgttct gctaatggtg aaacagtcag tttcatattg gctgttcacc ttctgccatc
      541 tttttgtatt tgtgcgtcag tcgcccgaat acctcttcag cacttttact ttcaccgctg
      601 ttgatgcctg cggtaacggc gctctggagt gattcaagct ttatctgctg ttcccgttct
      661 tccagagcac gaagtccggc acgaatgact tcacttgtat tgttataccg accactttct
      721 atttgttcgc ggataaacgt ttcaaagtaa ggactgaggg atacactggt aggcatggtg
      781 attttctcgc cgattgattg ctatcaatta ataatagtta atattcgcga attgtaaaat
      841 acctctttta tgccttgcgg cttttgttca ctgtgcttgc cagggcttcc gggtagtgtc
      901 tggcaataat atcattaagc ctgtcaacca gttctggttt cctgaacgtg atgtgtactg
      961 agcctttctt aaagtattta atactgaaca tctcgtcttc atagttctct ttaccctgaa
     1021 cagactggat atgatcatcc agacgaaggg tgatgttttc ccggttgtcg gggatcggtt
     1081 tgccgctgaa caggtgtagc attctttcca gatcggcaag tcgatctgcc tgttgcccgg
     1141 tgataaaatg aaacccccac cggtcccacc tcaccaggtt gttgacgata attttactgc
     1201 caaatttaca gggtgagttg gttttgtaat tccagctcag tcccctgaag aggttgatca
     1261 cccctcgctc aaacacctca tccttattct ggtgcagttg ttcaaatgtg ctcagaatgt
     1321 ttgccgcact gatttccgga aaattatcgt attccagtga ccggtaccat gtatcacgtg
     1381 cctgtgcatc cattaatgac agcatcccgg aacgttgcat caggtcacgc cagatttcgc
     1441 ggtcaagatt ccgggtaatg gctttcattg ctgtttcggg tttctccatc agccagcaac
     1501 cgcagcgaaa attctgtttt atggcccagt ccggggcggt tttacctcca atactttctg
     1561 tcattgctga gatttctgtc agttgccgga taagtgtgtc gatttgcatc agtgtggtct
     1621 ttcgtacagc cagaatgtgt ccgattgagg tggaacagat aacgtccgta tgcccgttga
     1681 ccacttcagg acgggagatt gtgatacctg acataatcga tattcctttt tgcagataaa
     1741 tgaaaacgcc agccctgttc aggctggcgt cagtggaatg aagtctggtg agtgatattt
     1801 accggttctg gtgcagaagc tgcagatgac gaagagtacg gacatcctcc cgggcctgtt
     1861 cgatacaggg caacagtgct gccgaatccg ttttatcctc atcgttgtca aagataattc
     1921 ccgattcgca gtcgatattg tcctgcagcc acgcaatgag gatatccagc gctgtttcag
     1981 tggttaatga tttcatgttg tttctggtta ccagtcgaaa gtgggtaaac ccggcaggca
     2041 ttcagcgctg gcatccagat gaatgagact gacgccataa cgccggataa gtgtgacaac
     2101 cagacggcgg aacatttttg acagccccag gcgtttaagc cgcagaaccg ggtatgacca
     2161 cgcatcggta cgtaacagat atccgctacc tgtaaaatga atccattctg attcgccgaa
     2221 gtcactggtc tggtgtgaca gcgagtacaa ccaggcgttg tctgtttccg tgatatgtgc
     2281 ggtgctgcag cgtatgccgg taagggttgt taacggtggt ggtgtgcagg ttgactgttg
     2341 gtcggagtcg tccaccacac ggagtgaata accgttttca gcgactttgt taatcagttc
     2401 agcgagctca atgccatcga cgtcaacgac aatgcgcccc atgttcagtg cctgcacgtt
     2461 aatgctgtcg gcttccagtg tcagggccag tttcattgtt gcgcctccgg atgcttaccc
     2521 tgggtaatgt tatttaccat tctgtaattg tcgcgggtca tcaggcctgt tgcccggcga
     2581 gcccggagga tatcgatgct gtttattaac tgagagcggg tacaggcgct gaatcctggc
     2641 tggtcagttc gtaccagcgc gtatttttcc acgagaaagt tcaccgcatc acacagtgaa
     2701 atgcctgcct caatatgctg ctcaatcaca cgttcattgg cgaacggtgt gtcattcagc
     2761 gtgaggccgt agtgctggtc cagcagtcgg gtgagcagtg tctgccagat ttcaaccgga
     2821 gacgggcagc gcgatgccgc ccgtacgtgt gtgtcgggta atgttttcat aatgaagatt
     2881 ttcctgatat gagcgattaa attttgagga gaatggtgct gtggtgcacc gggcagtagc
     2941 agaggtttat tctggtggtt tatgttttcg ttccgggtgt cggataaatg gcgatataca
     3001 cgtaaccaca actgcccagg gtatcggcat cgcaggtcag cccttttgca tacagcgtga
     3061 cggtatgctg atggcgggga ttcagttcac cgctggtgag catgagttcc agttgtttca
     3121 tcagcagcgg aaaggcttgg tccagatggt atgcatccgc atcgctgaac cggcctctga
     3181 tgtcggcgcg gtctgcaagg taatgcaacc ggttaccctc ctgcaccaga cgtgccccga
     3241 aacagggcgt cactgtgcag gggagtcccc accaggggcg gtcgtgattg tcgtcaggat
     3301 gcgttgtccc ggagagtgtg tctgacacga taaaatcctc acagaaaatc ggtgaatgat
     3361 agtttaacga tgacgggtca gccggcgcgc agaccggctt cggtaaaggt gtattcgttg
     3421 atgagaaggg cctcatccac agtggcatct gaggtcagcc agtcgtactc gttttccagt
     3481 tgacggtaca gccagaaggc caggtcgcgc agggcttcgc tgacagcggc ttccgcatcg
     3541 gttgtcatta tctgaccagt cggactgttc cgggtgacgg tgatatccat gctgtacgga
     3601 tggcagtagc gtccccggtg gtggatttca gcctgcagtt gccagaagtt acgcttctgc
     3661 accgcctgca gggcatccgc gatacgatga agctcacggt cctgcggggc atactcccgg
     3721 attatcctgg ctgttgctga ctgccagtgc cagcgtcctt caaaacaggc tccgtccccc
     3781 tggctgcaga atccgctgaa ccagatgcac ggctcttcca taaacctgcc gttgctgagc
     3841 tggaaaacgc gctggcggag gtcaatcccg aaaatgtcac agaccttcct gaagtcttca
     3901 tacacgtttt cataccagtt gctgtcagca tgatgctgac ggtacccgtc gcgggctttt
     3961 tcccgtgcgg tggcggatag ctcattcagt gtatacacgg tggaggtgat aattctgctc
     4021 atctgaatgc ccgtcctgat tcagttcagg gtgacgctca tcagccggac atacgggcca
     4081 aaactgtcct tacggcgttc agcaaacacg gccagcacac cgggaatatc ctgcacttca
     4141 cgaccggtat acgcctcagc actgccgtgc cagcggtatt tgccggtaca gaaaggaaaa
     4201 agacgggatg ccggatgttg ttggtgaata cgcatggctt caccacgggt gatgattttc
     4261 atagtgtgat acctctgaag acagaagata aaagtgaaaa caagcgtgat gtggttgtga
     4321 cgggttaaag cagaccatgt tcggcaaagg agaaaacctg gttgccaccg actatcagat
     4381 ggtccggcac ccggatatcc accaggccca gtgcctgtac cagacgttcc gtgataaggc
     4441 ggtctgcctt gctgggtgtg acttcaccgg acgggtgatt gtgcgccagc accacggcag
     4501 cggcattgtg gtacagggcg cgtttaatca cttcccgggg atggacttcc gtgcggttga
     4561 tggtgccggt gaagagggtt tcaccggcaa tcagttgatt ctggttattc agatacagca
     4621 cccggaattc ttcacgctcc agtcccgcca tgttcagaat cagccattcc cgtgccgcac
     4681 gggtggaggt gaaggccacg ccgggttcat gaagatggcg gtccagggtt ttcagggccc
     4741 gctgaatgag gctgcgctcg ccgggcgtca tctctccggg cagaaaggaa agttgttgca
     4801 ttgtgcttct ctccattcag tcgatgatgc gcataatggc gctgcattcc ggatgctgca
     4861 gggcgtaatc ccgcagccgg taataatgga ccgtcatggc ataacactcc gtacgacagg
     4921 catgatgact gtacgtcatc agacaggcgg caatgccggc ggcttccggg ctcatttcag
     4981 cgcggttacc gttcatggca ttgaacagta cccagttttc gccatcatcg tcatccggtt
     5041 cgggggccat aaatgccccg ccgttgttca gggtgtacag attccagata ccaccgcagt
     5101 agtcttcata cagacggtcc atccagccga agacccggga ctccagggtc acccactgtg
     5161 gaatgaggcc aaaatgctgc ggccagaagc tgatgcgctg ttcatcaggg acgaggctgg
     5221 caaccaactg aggctggtca ttccctgatg cagcggttac ggaaacagaa gatgtggtgg
     5281 aattatgtga aatggttttc atgaaatgac tccttaaagg acaaataaat gcgtggtttt
     5341 cagggtaatg gctgaatcag cgcaggctct ccagcagcgt ttccgccatc acccacaatg
     5401 cgcggttgag cttaatgtca gtgtcaatgc tgtgaatggc acgggtatgg atacgttttc
     5461 ctttagcgct gcgacctgaa attccgcctt tcagcatatt ctcctgaatg gtctgataag
     5521 cactccacag gtccttaccg taatcctccc ggcgtcgtgg cgtcagaatg tcggcggtgg
     5581 tgacgggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc
     5641 gtgccggtgg cggcagaatc agcgactgca tggcatcacg cttttcctca atccggtcaa
     5701 aaacccccac cacctcgtaa gccccttcaa tgactctgtc caccacattt ccccggtgcg
     5761 gaacacgcac ttcccccaga gactgaccac agacgcatcc gttctggcag acgaacctga
     5821 agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc agaataattt
     5881 cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg tgttttgtgt
     5941 atccccggcg gcccgggtcg cgcacacggg tctggcaggc gaagaatggc tgaaagcctt
     6001 cccgctgcag gctttccagt acggtgatgg tggggatgta cgtatagttc tggcttcggg
     6061 acgtgtgttt atcttccccg aaaatccccg gcacatagtg catcagttct tcgtgtgtca
     6121 gcggatggtc acggcgtatc tggttcgcat aaccaaaacg actggctagt cgcataattt
     6181 gctccttatc ggtggttaag atttactggt gtaataaatg aaaaagccac gtctcccgga
     6241 gaagacgcgg cctgatgggt gaaatgaatg acgtttattg tctgagaaga ccgtcactgg
     6301 cgagccgcgt attaagcacc tttccggcat cgggttcaga attccggcag cgccagcgtc
     6361 gctgaccttc cgcgttgcgg ataacccgac ggaaatgcat tcccgggtca tcctggataa
     6421 acacattgcg gtaagcggtt atgacagcaa ccgcctgccc gtatgagaaa gatccttcag
     6481 ccaggacata ctctgtgtaa cccggcatat ctgttttctc ctgataaata aaaagcctct
     6541 gccacgcgtt gtgacagagg ctgaagcatg aaactgactt cagggatcag ttaacgtttt
     6601 ttccggaaac ggtaatcagc agtggatggt agtcctgggg attgaaaacc gataacggca
     6661 gactgacacg atggccgtta ctttcttcag ttgctttaat gatttcggtt gtggcgacat
     6721 tttccacgca ctccgtttcc agaaatgcgt ctgtggttcg cgtggcatta ctgtcaccaa
     6781 aagcttccgt ttccattttt ctggtcacca gcgtctgacc atatttgtct ttgagttgca
     6841 gagtgatggt gagggggcca aatccttcat cgtttccgcc attatccagc cggaactggt
     6901 aagcacaaat atttcccggg agccatatcg tatctgtatt gcgtatactg atgtaacgtt
     6961 gatcctgtgc ccggagtggg gcagaccacg ttaaccccag aatgaaggcg gtaatcatgc
     7021 aggttttgaa caggtgaatc atggtattta cctctctgag tcatgacgat gacactgaca
     7081 aatcaggtga taaagcgtaa aaggcgcaga atagccgtta tgccggtaac tccgggggta
     7141 atgcttcttc cagtcggtta accatattgc cgagatggga tgcatcatat tccatgacgg
     7201 ggcgttgcct gatgatactg accaccagtg gtttgattaa catattggtc gcggcccgtt
     7261 gttgtatacc ggcggcgaaa atgatctcca gagcattctg atcgataaca cgggccagat
     7321 tcgcgttcag attttcaatc tcatgtccgg tcagcaggaa actgccggca agccatgtca
     7381 cctgctcctg aatcattgcc agaattttga cgcgatcttc ttcaaccagc gctccggcaa
     7441 tttttcctga tacagcggag gcaatcattc cccccagaac accgccaacc acacgccctg
     7501 tcagtgcacc aaatggtccc agtggactga acaatatccc gcccgccact gacccgacag
     7561 caccacctgc cacaccggaa gatgccacgg caagattcct gatgaactgc gcaccggaga
     7621 tccgtccccg caacattttg atcatgtcag ggccggttgt gacagctacc agtgccagag
     7681 atgtcaccag cgtacccttc atcactttgt tcagggcact gatatttcct gcaccggtcc
     7741 ccttctgaag cgcctgccgg acagtcgggc tggccgtcga aaaatcaata tgcttcagca
     7801 taccctgaac aacagtgagc cggtgaagtt gctgggttgt gacgtagaca gccagtgtgc
     7861 gggtgaaggt ttttcctgcc tggacagcag ctgtctgcag ggcagcatcg cgatcgccgg
     7921 tgctgagcca gaagatcgat gccgtcaggg caaaactgat ccctccggcc gccagactga
     7981 caaccgaccc ctcggcaata tcataagtga ccgattcgat ggtcccgaac cgggtgatat
     8041 tacgggcctg agtataagtc agatgtcccc gacgaatcag ccgggacgct tctgcgggat
     8101 cggttacacc gggtacttta ccttcgcgga tcttattcct catggtttca acagcgccgg
     8161 catactgatc acgggggact tccagttgca tggggccatt atttcccatg taacgatact
     8221 gtccgttttg tccgtcgaat gccgcaccga cgctacgggc tgcagttgaa caatatttcg
     8281 tctggatttc tgttccgctg accagtctgt ctgctccatt tctggcatta ttatcaccga
     8341 ggatccgtgc gtttttcaga cggactctgt cgacaatatg gtttccccgt tcagcggcaa
     8401 acccatgtcc ctgccctccg gaagcaaaca ttttctcatt tgtactccag gtttccggca
     8461 gtttcatgag aatcagacca ttcgccgttg catcggaaat aaagctgttg taagggcgtt
     8521 cataaatttc ctgacacagt gtttctgttt cgcgacaaag agaggagcag taccattccg
     8581 gatccggctt atgattttcc ggggcatgat gtacttctgt cagcccgcag gaggcacaga
     8641 tacgaagctc atccacgtga tggaatgata caaaacggcg accttctgaa aagggatacc
     8701 agtcaccgcc gtccaggcaa cgatcgagaa aatcgtcatt cgcttccggt gataaattca
     8761 ggcggttccg gaagagcgtt ttcagttcat caggtgtgaa tgacaggttc tctttcaact
     8821 gttcgttaaa ccctgccatg tttctttcat accaggcatt gttcagtaac cagtttccgt
     8881 ctgccagttc aaattccctc agctctcccc gggaaatccg ttgagaaatg agcgtcagcc
     8941 agatgcgctc cgtcagtgag tcaggaaggc tgtaatcgtt ttgcaaactg gtcagccacg
     9001 atttactgtt gagcaccggt tgttcttcca gtgtctggct gatggcacag gtcagttttt
     9061 tcagcgcctg gatctggctg gccgcccata gcagcgtcac taatgcagcc agccagtaca
     9121 acaacccgcc ccaccacatc agtgacggtg aaatatcgta ctgtaacgcg atcaatggtg
     9181 cgcatgcaga acacgccata aggaaaaagg cagtataaaa acagcagtaa tacccgttaa
     9241 tttccttgct gatatcttct ggtgtgatcc cgttctgctg aatgtttttg atctgcaggg
     9301 gaacagccag cagaccaccg gcgagagcag tggtcagaat aaatttcaca gagtcaaagg
     9361 tgtgagcctc cagcgcgata tgtttaccat caagtcccca catgaagctg atgatcattc
     9421 cggcaatcgt cagaaatacg cctgccagcc agcgaatatg gaacaaccgg gttatgcgtt
     9481 cggtgatata gccagccagt aaggccgggc cgaacagaca cagcaatatg agccctgcca
     9541 gcgcagaagc cgtgctgatt aatgctctga acaaaaggcg aagaatggca aatcctgtga
     9601 tcagcaggat cagcgcgccg actatctgta acatagtcac tccgtgatga atatcatgtt
     9661 attgttaatg ccagcgaatg tggcgttgaa tatccgttgc gccggtccgg gtcccggtca
     9721 tgatgaccgg gaccacagag aggcgatggt tctgtcagaa ggtcacattc agcgtggcct
     9781 gaccgttata gccttcagcg ctgctgccgc tgacgctgtg gacataacca gcctgaacgc
     9841 ccagggtgat gttttcccgg acacgggctt ccagtccggc ctgcaggtcc agtgacgtac
     9901 cattctgtga cggtgagaac gtcatgttac tgccggctgc ggctgtaccc atgctcatgt
     9961 ccccccggga actgaaggtg cggataacag aaggctgtac ccaccagttc accggcagtt
    10021 cacgcacact gtgtttggca ctgccgcgca gggtgtcacg ggatgaggtg cctttaccaa
    10081 agttcatatc gttgtggctg cccagacgga agccggcacg cacatgttgt gcactgccat
    10141 gcccgaactt cacataaccg gcgttgtcct taccgtcatc cagggagagt ccctgccagg
    10201 tgtactgcag ttgcggctcc agcatcagat tgtcagtgat actgaagggc agaccggttt
    10261 ccagtgaacc cagccagccc cggccccgga cgcggaagtc gttattgtcc gatgaggctt
    10321 tcatgctgtg gcgggttccc tgggccacaa tgtcagccca cagtccggag gcgttgtgta
    10381 tcagattcag gtatccgccc aggctgccgg catcatcccg gaccgtgccg gcacgggagc
    10441 tgtcatcatc cttaacatca acggaagaat ggcctgcagc accatacacc cctgtcgtca
    10501 gagacatacc ggcgacctct gttctgagca ggtcaccctc aagacggacg aagccatagc
    10561 tgccgctgct ttccggcgtg gccccacgga caataccgcc gttgttatcg tgaccgagat
    10621 ggccgccctg aatgctgaga cggacgctgt tgttttcacc atttacaccg gtctgatggc
    10681 tgcgggagcc tgccagaatc cggtcatagt ccattgcctg tgtcagcatg gaggcataca
    10741 gggggacttc tgcacgataa gcattttcac tgcgcagata ccagctctca tcactgtccc
    10801 ggttgaggga gtagttaaag gccccggcct gcagcctgtt cccctggaca aaggcccctt
    10861 cctccgtggt ggcaccgtta atggcttcca ccacctgaat acccttaccg ctggtcgcca
    10921 gccccgacgc actgttgccg gcgttcacca ggttcaggat ggtttttccg gttgccctgc
    10981 cgccgtcaat gaccagtctg tcagcattgc tctgtgccat atccgggcgt acacgcaggc
    11041 tgatggtgcc attctgcccg ttcaggtttt tcactttcag ggttgccggt acgaacttcc
    11101 ctgtgcgggt ggaggtgaaa tgaatctgtc cggcatggct gaggtcatcc accaccgact
    11161 gcaccgtggc gttatcaggg atattccagg tggcaccgga ggcgagagtg acattcgtgg
    11221 ggtcaatggc accgttcagc acagtgctgc cggtcagctt cagggctgta ccgcgctgag
    11281 caatgacatc cgtggtgacg gtactgtcgt tcagcgtcag cgtgccttca ttcaggttga
    11341 cggctttctg ggtgagtgtg gtgttgctga cagtgagtgt gccacttcct gatttttcca
    11401 cgctgccgtt accggtgaga gagcctccct gcaggagtgc atcgccttca cggatggtca
    11461 gggtattgtt gttcaccgtc ttcccggaaa gggtaagtat ggcgccgtta ttcagcgtgg
    11521 tggtgcccgc cagtgtgccg cccctggcgg tgaacagtcc gccatttacc gtggtatccg
    11581 tggccgtatc accgacgttc agcgtgaatg aactgccttt ttccagcatc agggcatccg
    11641 cctgaccgcc tccgatactg aatgcctttc cgtcgctccg tgtaccactg acagcggcac
    11701 cggaatcggc cagcagtaca ccgccatttc ccatggatac ggtggtggcg gtaccaccgt
    11761 tgcggacatc cagcgttccg ccatcatcca cgcgggtatt agtggctgtg tgtccggtca
    11821 gcacatccag gcgtccgcca ttttccagta cgacattatc agctttaccc tccacaaccg
    11881 agaatgctcc aaggcggttt atgccggtaa cggttgcagc cgtactggta accagtgcac
    11941 cgcccgtgtt ctgggtgact gcagtggctt ccccgccggc atgtaccctc agttcaccgt
    12001 tctgatttat ggtggtgtta ccgacagcac cacctgcctt aacaacctgc cagcccccct
    12061 cgttaattac cgtgttcagc gcaagtccgt ccttgtgcac atactggtaa ccgccattca
    12121 gtgtggtatt cagggccctt ccgtgtaccg actggtcacc acctgcgtaa atgagagtgt
    12181 cacgggctat cccggaaaat aagataatct gccgtccatt tttgttgatg gtggtggatt
    12241 ctgctgttcc ctggaccttc tgacccgtgt acgagttgtc agaatccggg ccgccttctg
    12301 cgccggtgtt gatgatggtt cctgttgcca gcccgccact tttaacgctc tggtaaccgt
    12361 cgcggttgat aattgtaccg gtggcaaccc cgccctcatg aacccactgc tcgcctctgt
    12421 tattcagcgt ggtgttcacc gccagcccgt tcaggctctg tcccccgccg tcacgaataa
    12481 ccgtatcact ggctgttccc ccctccagca cgacctgacg accatttgtg gtaacagtgg
    12541 tgtttccggc tatcccgcca ttctgtatcc attgcccacc ggtgttttct tcactgtccg
    12601 gccccagttc cagcccggta ctgatggtca ttccgttggc cgtaccgagg acaatctggt
    12661 tgtcatgatt tgtcagtgtt ccgtcgttca cggtttctcc cgcctgtaca accgtgtcag
    12721 cagccagtgc cgggactgat gtgacagcag caagagacag cgcaaccgcc acaccggcgc
    12781 gttttccccg tgagcgggcc agttcggagg ccaccaccag ggtgcccgta atgtgattcc
    12841 ataccagcct gtagctggtg ttcagatgtc gtttcatcag cttttcctta cagagggtga
    12901 ataaaaaagc cggtacccac aaatgtggat accggcaggt acaaaacagc atggtcagat
    12961 aaagtggttt ttcccggaca taaatgcttc ccctctctca tccgccagag aagtatcagt
    13021 ccgttgtgtg caattccgtt ctgcatgatg aagagattca gctgcctttt cgcagaccat
    13081 taaccactat aaacgatcga tagtaacgat cgatacctgt tttatcgatc gttttattct
    13141 attgacagca gagcggtcac gcaagagaaa caggaggggg atgaggggtt tgtgtcatgt
    13201 tttcagttgg ttaacgcaat gtaatcaggc atttcccctg gcagagtgtt cagaagaaaa
    13261 tccagttcca tacagcgcgg gcaacagaga ccaccgagtc acggacggca cgcagaaccg
    13321 tgcgtgcaac agaggcaata cagacgctct ccgccgtgtc aaatatccgg tccaccgcac
    13381 cggtaaactg ttcacgggcc tgagaccgga cagattccgt gcgcagctcg tcctgcagtc
    13441 gggtcatcag gggactggcg gcatggtcgg gaagcgctgt catgagcgca ctgaccagcg
    13501 tatccagttc ccagccggtg cgggccgata cggccacaac cggatgtacg ggccggaaca
    13561 gacggaatac cgcctccgtt ttttcgcgaa tattctgtgc ctgtgcagga gaaggctgaa
    13621 taccggccat atcccattca tggcagggct ccgttttgtc ggcctgcgtc accacaaaca
    13681 gtacccgctg atgtccccgg tgcaggatgt gtcgccagaa atactcatcc acagacaggg
    13741 cacggtcatc ggctttaatc agccacagta ccaggtccag ttcaggcaga atgtcacggt
    13801 acagggcctc atactctgca tccctgtccc ggctctcgcc caccccgggc aggtcagtga
    13861 taaccatgct gtgaccgtgg ccactcagac ggaagcgccg tacttcccgg gtgccggtgt
    13921 gaacatcact gaccggggtg acctccccct gaaacagtgc attacagagt gaggatttac
    13981 cggccccgct tttacccata atgccaatca ctggttcgtg acgggtgagt ttgcgcagat
    14041 gttccaggat gtgacgggaa agcgagtaag gcagggagga gagcggtttt tcaattgcct
    14101 caatggcatc agacggattc atacatgtat tcccggtgaa aaaagacaaa accccgcgca
    14161 ccggaaaggt ggcgggggat tcacggagat aatatctgtc tgaaattgtt ttgaatcgat
    14221 gggattgttg cgtattaaca tgaaggagat caggacttat ccgttactgg gtgatctcct
    14281 tccatgaaaa tacgccgtta ttgttaaaca gcaggctgat ttctccgtaa aggggaaaat
    14341 atttatcggc tgcatgatta tcaggtgtgt ctgtgtttac tgctgtgagt atttttcagt
    14401 gtattgtggg ggagatgtca gtgggagata ttaaatttac gtttataatt atcttacttc
    14461 ttctgtgtgt tttttataag agtgcacacg taatgatatc tgttgatacc atgtggttaa
    14521 gtgtttccct tcctggtcat gcgtaatgat taaatgcttt tgtgacacca ttcttccggt
    14581 ctgaatattt atccagttct tttttatttg tgcagtgata ctgtcggaag agaattcagt
    14641 aatgtgaggt gtcctggtca ggataatatc tgctgagata ttattagttg agtgtgtttt
    14701 tactgagcaa aagctggctt ttaattcctg tactgaaacg ctgtcgctcg tccggggtta
    14761 actgaaactg gatgatgatg taaaatgaag gctgtttttg tgtttgtcga agaagtgtta
    14821 ttttaatcca ttctcatcat tggctggcga ttatacagaa gtatttgaag aaaaaatggt
    14881 gcattttatc tgcgtcagca gggaggaagt attttattga tgtatcagta ttaaatggtt
    14941 tttgttattt ataaatatgt catcctgtcc atagaatatt gtactcagca ttcagtgcag
    15001 gaactgtagt cttctttatt ttattcttct tcaggagggg gggattcctg aaggcgttat
    15061 tcccggtgtt ctgtttatga ttcttcccgg caggaaaccg gtatctgcaa tacccgggat
    15121 gatgcttttt gttcgtacgt ggttgtgtaa aatttgaata gtggatgtgc tctgaaggga
    15181 aggaggggac acacagattc attttgtctt tcttcagcag caaaacacac tgtggttatt
    15241 atttaaccat taaagcaaca aaaattttta cctcatattg atttgactgc tatttttgct
    15301 attaatagcc agaattgcag tacttttata tctgtgtgta gtgaggttac tatgtcattc
    15361 ggtcagcctt gtgatgagtt tcccctgagt tcgctgcctc cactcattcg tgatgccgtt
    15421 attgaagctc agcaaattac gcaggctccc cttgggcttg tggcagcgtc tgccctgggc
    15481 gctgtttcac ttgtctgcca gaatctgatt gatgtctgca gactgaatac actgcggggg
    15541 ccggtctctc tttttttcct gacgcttgcc gagtccggag aacgaaaaac cgcagtggac
    15601 aaattattaa tgaagcccct gtaccagcag gagatgcagt tatattccag atacaaaagt
    15661 gaactggctg tctggaaaaa caaggaagag cttttaaagg ctcagaaaaa agcattgttg
    15721 tcaaaactga ataaagaact ccgcaaaggc gcggatgagt cagaaaccct cagacaactg
    15781 gaagtacttc agaaaaacag tgcggaagaa cctgtgaggt ataaattcat ttttaatgat
    15841 gccacgactg cggcaattaa gaatcagctt tgtggtaaat ggcgttcagt gggtattatg
    15901 tcagatgaag ccggtattat atttgatgga tataccctgt ctgagttgcc ttttattaac
    15961 aaaatgtggg atggctctgt attgtctgtg gacagaaaga atgaacccga acagatgatt
    16021 gaaaatgcca gaatgacgtt atccctcatg gtgcaacccg gtctttttga ccgatatatg
    16081 gagcgaaaag gttctgttgc cagggacagt ggttttcttg cccgttgtct tatcagtaaa
    16141 cctgcaacca cgcaggggaa acgctttatt aatggagctg tgattccggg aggatcatta
    16201 acggcatttc atgaacgatt aatggaactg gccagaggca gtattgaaaa atccagtgag
    16261 gatgaacggt attgtctgca tttttcgcct gaagcgcaaa aaatatttat tgagcactat
    16321 aatgttctgg agcaggattt atccccttcc ggacccttaa gcccgtttcg gggacatgtt
    16381 tcaaaaaaaa cagaaaacat tgcaagaatt gcagcgttat ttcagtattt cagctatggt
    16441 gaagggaaaa tctctgccga tatcatgacg tctgctgtcg taataagttc gtggtacaca
    16501 gatgaatata aaaaattatt tgcgttacct aatgaatctg aactgcagca gaaggacgcc
    16561 gaggagctgt tcgactggct cattgaggag tgccgcggag aatgcccgcc ccgtgtcagg
    16621 aaaaattata ttctccagtg tggcccgggc cggttcagaa acaggaaaaa actgaacgca
    16681 ttgctgaaca ttcttgaatc tcagttcagg ctttctgtcg tgccggaagg aaaaaccatg
    16741 tatgttctgc ttccccagat agccagttta aagttgtctg atgtctctgg tatttttacc
    16801 tcgggttatc actacaataa gctgagagcg aagtaaccgg tgagcagttt ttctccatta
    16861 tgcttcagac ttagatgatt gattattatg gggatttcat caccgatatt cagatgtatg
    16921 aatatcggtg atgaactaaa gctcaggaac tgcgtggtgg tgatgaatca acaggcatat
    16981 aactgatacc cgccggtcag aagttccagc ctgcgtcttt cctgtctgcg tgcccgcagt
    17041 ccggcgttca ggtgcagcgt gtgacagaac gcacggagag cgaaacggca ggcagacagg
    17101 ccgttcagtc agccctggca ccctgcccgg ataccagctt ccctgaaaca actggcatca
    17161 ggtgcgctta tcgtcaaccg ttgtgcggta tgccaggagc acgtactgat ccatcagatc
    17221 cgtcagtgct gcgctctgct tctgacggtt gatgatccat tcctccagct ctttttctga
    17281 tttccgtttc agattctccg tccagtcgtc aaggcagttt ccggtcatta aatcaagcca
    17341 gcaaagccag accagtttga ggcgcaactc cgtgccacca aactgacgga agaggctgat
    17401 cagtttattt gccagcgaac gctgcctcag acgttcacat tcagacagga gaatgccttc
    17461 cagcatcgga tgatgaacca gcagatgttc actggtgcat aaattcacta tcagatcatc
    17521 atccaggggc ggcgtggtcc gtttgtccgg accatacatc agccggtgca gactctgggg
    17581 tacgatggca atctccggtt tttcaccatt gaatgacacc cacccgggcg tcagattatg
    17641 gaagtagtag atattcggat gacacacgcc ccaggatacg gcgtgccggg tgaagccggt
    17701 cagccatttc tggtagcttt cgtggagcag ctcgtaacgg gtctgatact gtggtttcat
    17761 tttcatattc ctcacggggg ataacaggca gcagaacgcc gccggcaggc cggagactgc
    17821 gtctgctgca gatgaacggt taagaattac ggacgggact gtgcaatacg cgcctgcagc
    17881 caggcttcca cttcgctttt cagccatcgg gagctgcggc ccattttgat gggcgcagga
    17941 aagtcaccat ccttgatgag tttgtaaaac cacttatcgg tcaggccggt cagttgagtg
    18001 ataaatgcca tgtcgaccat ctggtcatcc atcagtgaaa ctggggtgat catgagaatg
    18061 ttcctccgga tgttgttaaa ccagccggtg tggtcatgaa aacaacgcac caccttcacc
    18121 ggcataacaa ccggggagaa acgtaaaaaa taatcgggaa tgtgaccgcc aggagataaa
    18181 cggggaggtt acggtggctg agccgtaccg gtatgccggg tggttgttac gaaacgatgc
    18241 ttccgcacgc gcgcgcgata aagaagggat gattttcgtg aggaagaggg gaaaactggc
    18301 gttctgataa ttgcagagtt atttacgggg cggttacgat ggggcagacc gtgtccggtg
    18361 ggcaggtgac aggtatcacc gatgggataa gactgtctga ttggtattat aaatatataa
    18421 gagagatcaa acacatgaga tcaaatacat gagttcatat atgtgaaata aacacggtga
    18481 gtgtgatcag tgggcatgat gagaacgcag tagcaacaga ctgcgctatc cctgatagct
    18541 ggcgtataac ccgcgtatat aaaacgtatc tgcaatgtac gtgaacagag tgcattaagc
    18601 atccgtctga gcggtaaaca tatccgacct ggtgagtgac agtctgagag accggttaag
    18661 tggtggctcg ggagtaatga gtttggggtg ggtaccagaa tgccagtctg gcggtatgct
    18721 gacagtatca cgttaattgc agcataaagt gatgagaggc taatggaagt taaaaccaaa
    18781 gcagttccgt cgttgttcat cccgggagac gctggaaaaa gtgatttccc acacgcgtta
    18841 taaacttacc tctgcggaac tggaagcctt caactctgtg gttgatcact ggctggcaga
    18901 actgacaatg aacaagcttt acgatcgtgt tcccgcttca gtctggaaat atgtcatctg
    18961 agcgttcggt aatcacaact gcatcgcgcc aggtgttatc cgctttgtga ttcttattgg
    19021 ctcttctcag gttattaaaa gctgaatatg ttcctgttgt cgggaaccag gataataccg
    19081 ggaacgttaa gcggtatagc gacaacaaag aattcgaaat ctaaagggaa tggtgccgga
    19141 ggcgtgggcg gtaatgggct gattttcagg tattaccggc ttacagcgtg tggttgatat
    19201 tgaggggctg tgagtttcac gtatttgtca gaaacccaga cgagttttcc tgtatgagac
    19261 gaaataaggc agcggcaata cctggaaaaa tttaatatgg tatgaaggag aacctgtcgg
    19321 ttacgcttaa tatacccggt acactgtcag ctcaggggat acatgaaccg ttatctcacc
    19381 agctacacag tatatacaca ctgacgctga cagtagtcag caggaccaca ataatcagtg
    19441 ccagcagtat agtaacgata cggtctgtat tccggtaata gccgaagggg tcgacagaac
    19501 cacaatacgg gcaacacaca gtattatgag taatcgcgtt tccacagtcc cagcacttct
    19561 ttttgcgtat caccataact ccctcctcac agccttatcc gtaatcggtt ttttacatta
    19621 aaaaaacaac gctgaaaatt caaccgtcag tccggagacg accgttcggg ttatctcaga
    19681 gtgcctgaga tattgcctgg ttggagatca cttcagggac tctgcgcatt ttttacggcg
    19741 tggttaatcc gtatgaacga aaccatacgg agatttaacc atgacctaca aatacaaccc
    19801 cttctggcag caacgtattc gtgagacggt gcggcacgca ctggatgttc atccccgtct
    19861 gacggcattg cgggttgatc tgcgtctccc ggatgtaccg gcagcaacgg acgcggctgt
    19921 gatatcccgc ttcatcaatg ccctgaaagc ccgaatcgac gcttaccaga aacgtaagca
    19981 tcgggaaggt aaacgcgtgc attcctcgac cctgcattac gcctgggccc gggagttcgg
    20041 ggagcttaaa ggcaaaaaac actatcacct gctgctgctg gtcaaccggg atacctggtg
    20101 tcgtgccggc gattaccgcg ctccggggtc actggccggg attattaaac aggcctggtg
    20161 cagtgccctg ggagtggatg ccggacgtta tgacacgctg gcacattttc ctgacaagcc
    20221 agcggtgtgg ctggaacgcg atgatgaaac cggctttcag caggtgctgg aacgttctgg
    20281 ctatctggcg aaggagcata ccaaagctcg cggcaccggt gagcgcaatt ttggctgtag
    20341 ccggagttga acacgacagg cgcaccaata cccggcaaac caaaagtatg ggttgttgtc
    20401 tggcggcaac gatgtatcca ctcgcccact gactgttttt tacgtcagtg ggccattgtg
    20461 tgacaaccac agtgctgtaa cagcaaccag cctgctttac ccaaactcca gtccagattt
    20521 taactgaacc gccattcaca ccctgaccac gctgcccgta cctggacggc tgttgcctgc
    20581 gtgtcgtctg gcggctgagg aatatatact atgtacgcaa aatcctttct cgctcttgat
    20641 ggcaacggac gtctgacggg ggcccgtacc gcacagactg cgccttacga ccgctacacc
    20701 tgccaccttt gcggcagtgc actcagatac cacccgcaat acgacactga acggccctgg
    20761 tttgaacacg ctgacgaagg gctgacggag cacgcgcagc aatgtcctta tgtcaggccg
    20821 gaacgcagag aaatacagtt gattaaacgt ctgcagcaat tcgtcccgga tgccttaccc
    20881 gtggtgcgta aagccagctg gcactgcaga caatgtcacc acgattatta tggggagcgg
    20941 tactgcacac actgccagac cggacgcttc agtgaggagg ggggtagcag aatgaacggt
    21001 ataaaaacgg ggctggggat cacgccgggt gaacacatta tcagtgccga ctctgcgctc
    21061 agcagaaata tccggcattg tttctgcctt tcctgccatg gccggttgat tctgcagaca
    21121 gatgctcagg gggcatggtt tgagcatgat ttacatgccc tgagtgagca acagaaggcg
    21181 gactgtgtgg tgctgaatcc ggaaaagtcc cacccttaca ttgaggatat ggcgatgttt
    21241 ttatcgccat tatcggtcgt gctggagtgg cattgtgtga tgtgtgagca gttttttcat
    21301 gggaagaagt tctgtgaggc ctgtggtacg gggatttatt gcagagcggt atgtacgaag
    21361 tctgtttaca gctaccagcc agacttgttt gaggactgcg gaggtgcgag tacttaatcc
    21421 gaaatccccg ctctgcgcag ggcgttcaga ggtattgcca acctgtattt tatccggtgt
    21481 ttcgcaccca ccccgttcgt gacacaacgg cgcgacaccg gatatccgca gtcctgctta
    21541 cctgaataaa acccggggta ttgattggta gtgtggtgcg gaatcgtacc gtgatctcgc
    21601 atccgccgaa ttcgcactgt ggtgtcaatc aaccatacgc gagagtcctc tccgtattta
    21661 tcagtgcctc aggatgattt ctgcatagtt aatccagggg gctgcattgt tcatttccct
    21721 gctaatggac gtgacatcca ggatattaat gctcctgatt tccctgagca gtatcagcgc
    21781 ttattggcac gtctggatta tttgacgaag cccgatgcta aagcatcagg ccagcgtaac
    21841 tctggctaca gcggtttttg acctgttgta actgtaaggt gatttattat gtatacagaa
    21901 tcaaatgtta agctttcctg taacgccctc tggcaaaagt gtgtccttgc aactctgtat
    21961 cgggctgcag ccacacatcc tgaaattaaa ttattacaat ttaaattaat accagggcag
    22021 ggtattaata aggccattcc ctctctgatg gctgatttta ctggtgaact gcatagactc
    22081 atttctgaat atttgcagtg taagcgacgt aaagatcaat ctgttgagcc caccccttta
    22141 tattatctct ggagtaggga agctgtggct aacgatacca ctccttatct tatggtgctg
    22201 ctacttaata acgatgtctg gtgtaagagt cgtgaggtgg tggttctaag aaatctgatt
    22261 gagcaggcgt ggggaaaagt attgagtgaa gataactcca ttacacatct ggttgtgtat
    22321 cctgaagtgt tgtcgttggt atttgatgca gctaacacat acaacgctga gcctggtgta
    22381 ctggaacaga cggcccgggc ttacattgaa tcctatggtg ccattgaacg tcatcttggg
    22441 tgtagtgact tttaacgtca ggtgacgcat tgtcacttca gacctgctac ctgtacgccg
    22501 gaatataatg gtcagagagg catatttcga cgcccctaat ctggggcgtt tgattcagga
    22561 tgtctggaac aatccgggct ttgtgtcacc gggcgcatca aagctgtacc acccgggatg
    22621 tggcgctgga tcgtcattta ccggactgcg aagacatact taaagaggtg atatgggcgt
    22681 tcagtgattt tgtccgcgat aatcgtggtg tgtatgatcc ggaagcccgt taccccgccg
    22741 gtaatccctg gtatcccgta acaggccagt tctgaaagca cagctcagca gattccccgc
    22801 attcgcacaa tcttcataaa tctcacagga cattctgacc atgttgacta caacaagcca
    22861 cgacagcgta tttctgcgtg ccgataattc cctgatcgac atgaactata tcaccagttt
    22921 caccggtatg accgacaaat ggttttacaa gttgatcagt gaaggccatt tccctaaacc
    22981 catcaaactg gggcgcagca gccgctggta caaaagtgaa gtggagcagt ggaagtgaag
    23041 tagagcagtg gatgtagcaa cgaatcgagg aatcctgggg gcagcagcat gaaacgtgtt
    23101 gtaatgccgg tacgctggca atgtgcaaaa tgccagcgct ggtattgtgg aacccggccc
    23161 tgcccttggt gctggcgata ttcccgttca tcttcctgct gataccttcc gttcagcagc
    23221 gggttgttat cccttcctga ctgattcgtc attccattct tattgattag aactggcatt
    23281 acaccggtgc cggcgtgctt tcctgcgtgt ctgcgccggt tcagtaaatt agaaaagaaa
    23341 catttgtcag cgccagtgat ataaggcgta acggctaaca atcttggcgt taatacattc
    23401 cgagcaaata accgaagcgt tacttttagt gacggggtta tccaatactt ttctagtcag
    23461 tgtttagtat ttatgcaagc gatataagta gtcaccgctt agactattga cattcatgat
    23521 gcctgttaat tcaaatttca ggtatgaaca gtcatttatt gttgtgatac tgattccgct
    23581 agtttagcat aactgtcctg caaatcattg ttcaaaaaag aaagctccta caaactgtat
    23641 cctgtatctc taccagaaga taatatactt ggttattttt gtgcataagc tcacataaaa
    23701 ataatattaa ttatgttttc ttagagatag aaaaataaac aggtaagcag aagacggact
    23761 tttttaatgc gaaaacgtat tctttttatt ggcccaccgc tgtacggttt gttataccca
    23821 ttgatttctc tggctcaggc ctttcgtgta attggacatg atgtagtaat tagtagcgct
    23881 ggcaaattcg cgaataaagc agcagaagct ggactggttg tttttgatgc agttccaggt
    23941 ttagattcag aggctggata tcgccatcag gaagagttga ggaaaaaaag taatattatt
    24001 ggtcatttct ctttttttag cgatgaaatg gcagataacc tcatccattt tgcaggaaaa
    24061 tggaggccag atttaatagt ctatccccct cttggtccgg caggcccatt ggttgctgct
    24121 aaatatagaa ttccttcagt gatgctggct gttggattcg cgcatacatc tgcccatatt
    24181 cagatgttaa accgttcttt aagcaatgct tacaggcggc atggagtcag cggtccacta
    24241 tgtgatttag catggattga tgttgctccc ccaagtatga gcattcttaa aaatgctgga
    24301 gaaccggtta tctcaatgag atatattcct tataacggag gtgctgtaaa ggaaacatgg
    24361 tgggacaggg attctgatcg gaaacgttta cttatcagcc ttggcactgt aaaaccaatg
    24421 gttgatggtc tggagctgat ttcatgggtt atggattctg caaatgaagt tgatgctgat
    24481 atcattttgc aacttgcaat aaatgctcgt actggtttac gaaaactacc atcaaatgta
    24541 cgtctggttg actggatacc tatgggtgta ttccttaatg gagctgatgg atttattcat
    24601 cacggtggcg caggtaatac cctgacagcg ctgtatagtg gaataccaca gattgtgttt
    24661 ggcgaaggtg cagatcgctc tgttaatgca gaaattgttg cgaagcgtgg gtgtgggatt
    24721 attccggaca agcatggact gactagtgat ttggtaaatc gcctgcttta tgatgattca
    24781 ctacgcttct gttcagatca ggtagccgct gaaatggctg aacaacccag tcctgcagag
    24841 attgcagagg ttttgatgag aaaattaaaa aacaacggga aacaattgta gcgtattgct
    24901 gaaattttca acgagaaatg ttatgagtaa tataatgtcc ttcatcacat aaatgaagga
    24961 catcactaaa taaatgagat agtctcccat aataatattg cagaaagagt tatcggacga
    25021 taaaaagata ttttacacag agttattatt tgataccttc ctgcaattat aatttgctaa
    25081 ttcatcaata attgctccgc gccaccaagc ataatcgtga ccaccggtat agacagtgag
    25141 gttactttcc aaaccagctg cgattaacga atgatgcaat cgctgaacat gtgagacggt
    25201 tgtaccttct aaagaaccga ctccaagtcg aatatttaca tctttcggag gcgctgaaag
    25261 tatctgctca cttacccagg aggtattatt ctcagtaaac aagatcgggc tgccctggtc
    25321 agggttccac cacattgaag gtgagtgact aatgattgta ccaaatgttg tcgacgcata
    25381 tatagctgcc atcagtgctg tcactccacc gaggctctgc ccggccagta cagtattaga
    25441 gcgaccagcc catacgatat ccggatagtc tctgtataac tggggtatca gattttccgc
    25501 tatatcaaag atgagttctt tattgcctcc cagtatattc atacgatcag attcattaat
    25561 attgtctatc cccataattg ccattggcgt gatacgacca gtggtgatgg ccatatctaa
    25621 tgcccgggta atatctatcc ggtcaaacca tgtttcagca tcaggtaaca caagtaatcc
    25681 aagaggtact gatgttggat tcaggggaaa atataaacga atccggcgtt gatatcctgc
    25741 aacaaaggaa tgtaatgttg aaagaatacc tgtacattta tgggaagtat catcccattc
    25801 cttttgttca ggagccatat caagagaaag gactgattct ccgaatcctc gtatatttat
    25861 ttctgctgtt ttgttaaatg gatcagattt accgggtaat ggagagaagc gacttcctag
    25921 ttgaaatatg tctttttgtg tcatatctgt gggaatttct ataaatgagt atgagccccg
    25981 atatgaagct ggtaacacca gtgtcagcat ccagatatct gtcgaaggga tatgagtcat
    26041 tagtcctttt ttgacgtctt ttttatctgt tacacgattc aggcgaagat atacgccttg
    26101 cagtgctttc tctgagcgcc acagaaatgt aacctcccga ttttctgtag tggtgctttt
    26161 tgtgatcact ggaagtgttg ctgactcaag atagtgccag aaattagcaa tatctgttgt
    26221 tcttattgtg acaggatgtg tcattttttt taactgtaca caaatatctt cgggtgtctg
    26281 gaaaagatag tttttcatag gtatcatctt aaattaatcc aagtgtaaga ataatacatc
    26341 aaatgacgtt cattcaaggg atgatgagtg actttccgga attaaatctg ttgtattgat
    26401 gataaatata aaacaggttt aataaaattt ttgctttata ctgtagattt cacgtatgta
    26461 agtaatatat gaaaaggctc tcgttaagat gtctggtaca aaatgacaga tcatatgcct
    26521 ttgtaaagag acgacagaag tagagtctta caggcacatc ataaataccc tccggccatg
    26581 ccggagggta tttatttaac tgccgctgtt tgcactggat gtggaactac tcatactata
    26641 agaaggtcca cttccccccg gatacatgat gatggatgga gtacccggag cccagcatgc
    26701 tccattagca tcaatatatg ttcccatatc ttttcctcgt agatctatta cattagtaga
    26761 actaggtaat ccattaagat tcatccctcc tttcacgaaa tcaagcatgt gatctgatat
    26821 ttctctcatt ttaaagacct cctgctcatt tatgttcaga tattaatttg gtgttgcaac
    26881 catttattat cacagaattc agaatatcat tcagttttat ttttgttaaa atatcccctc
    26941 ttaagacaga ca
//
LOCUS       DAZGBC010000057        26866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGBC010000057 DAZGBC010000000
VERSION     DAZGBC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.057"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..257
                     /locus_tag="ACHK1J_002317"
     CDS             3..257
                     /locus_tag="ACHK1J_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911570.1"
                     /translation="MTDKNDCARIEAISGLAERKDNRVITAIIYELQKNIIFDEVIIL
                     AGILGDVKLHPILKNILNEFNDEDVIGNIKSAIQQIIKYN"
     gene            431..940
                     /locus_tag="ACHK1J_002318"
     CDS             431..940
                     /locus_tag="ACHK1J_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO7911571.1"
                     /translation="MNLQRFDDSTLIRIFALQELHRLKERGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQNLDDYYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            937..2355
                     /gene="phrB"
                     /locus_tag="ACHK1J_002319"
     CDS             937..2355
                     /gene="phrB"
                     /locus_tag="ACHK1J_002319"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO7911572.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVELVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHCPFIAWTERVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2505..3986)
                     /gene="dtpD"
                     /locus_tag="ACHK1J_002320"
     CDS             complement(2505..3986)
                     /gene="dtpD"
                     /locus_tag="ACHK1J_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO7911573.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            4257..5000
                     /gene="ybgI"
                     /locus_tag="ACHK1J_002321"
     CDS             4257..5000
                     /gene="ybgI"
                     /locus_tag="ACHK1J_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO7911574.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5023..5679
                     /gene="pxpB"
                     /locus_tag="ACHK1J_002322"
     CDS             5023..5679
                     /gene="pxpB"
                     /locus_tag="ACHK1J_002322"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO7911575.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5673..6605
                     /gene="pxpC"
                     /locus_tag="ACHK1J_002323"
     CDS             5673..6605
                     /gene="pxpC"
                     /locus_tag="ACHK1J_002323"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO7911576.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDQLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6595..7329
                     /gene="pxpA"
                     /locus_tag="ACHK1J_002324"
     CDS             6595..7329
                     /gene="pxpA"
                     /locus_tag="ACHK1J_002324"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO7911577.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7365..8156
                     /gene="nei"
                     /locus_tag="ACHK1J_002325"
     CDS             7365..8156
                     /gene="nei"
                     /locus_tag="ACHK1J_002325"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO7911578.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8153..9199)
                     /locus_tag="ACHK1J_002326"
     CDS             complement(8153..9199)
                     /locus_tag="ACHK1J_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO7911579.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VNLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9348..10409)
                     /locus_tag="ACHK1J_002327"
     CDS             complement(9348..10409)
                     /locus_tag="ACHK1J_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7911580.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     PLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     APIDVGPFDTVVLFKINYN"
     gene            complement(10406..11137)
                     /locus_tag="ACHK1J_002328"
     CDS             complement(10406..11137)
                     /locus_tag="ACHK1J_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7911581.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKAASQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKNPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(11152..13611)
                     /locus_tag="ACHK1J_002329"
     CDS             complement(11152..13611)
                     /locus_tag="ACHK1J_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7911582.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALAAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWLGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITPLNLNLYANILHQSWWN
                     ADTSTTANITAGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNSTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSTGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13661..14227)
                     /locus_tag="ACHK1J_002330"
     CDS             complement(13661..14227)
                     /locus_tag="ACHK1J_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7911583.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGNGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVVLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14614..15897)
                     /gene="gltA"
                     /locus_tag="ACHK1J_002331"
     CDS             complement(14614..15897)
                     /gene="gltA"
                     /locus_tag="ACHK1J_002331"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO7911584.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(16103..16210)
                     /locus_tag="ACHK1J_002332"
     CDS             complement(16103..16210)
                     /locus_tag="ACHK1J_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911585.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16591..16995
                     /gene="sdhC"
                     /locus_tag="ACHK1J_002333"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16591..16995
                     /gene="sdhC"
                     /locus_tag="ACHK1J_002333"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7911586.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16989..17336
                     /gene="sdhD"
                     /locus_tag="ACHK1J_002334"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             16989..17336
                     /gene="sdhD"
                     /locus_tag="ACHK1J_002334"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO7911587.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17336..19102
                     /gene="sdhA"
                     /locus_tag="ACHK1J_002335"
     CDS             17336..19102
                     /gene="sdhA"
                     /locus_tag="ACHK1J_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO7911588.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            19118..19834
                     /gene="sdhB"
                     /locus_tag="ACHK1J_002336"
     CDS             19118..19834
                     /gene="sdhB"
                     /locus_tag="ACHK1J_002336"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO7911589.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            20135..22936
                     /gene="sucA"
                     /locus_tag="ACHK1J_002337"
     CDS             20135..22936
                     /gene="sucA"
                     /locus_tag="ACHK1J_002337"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO7911590.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22951..24168
                     /gene="odhB"
                     /locus_tag="ACHK1J_002338"
     CDS             22951..24168
                     /gene="odhB"
                     /locus_tag="ACHK1J_002338"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO7911591.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            24444..25610
                     /gene="sucC"
                     /locus_tag="ACHK1J_002339"
     CDS             24444..25610
                     /gene="sucC"
                     /locus_tag="ACHK1J_002339"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO7911592.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25610..26479
                     /gene="sucD"
                     /locus_tag="ACHK1J_002340"
     CDS             25610..26479
                     /gene="sucD"
                     /locus_tag="ACHK1J_002340"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO7911593.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         6553 a         6684 c         7298 g         6331 t
ORIGIN      
        1 tgctgacaga taagaatgat tgtgccagaa ttgaagctat aagcggactg gcagaaagaa
       61 aagataatcg tgttataact gctataattt atgaactgca aaaaaacata atttttgatg
      121 aagttattat tttggcaggt attttaggtg atgttaaact acatcctatt ctaaaaaata
      181 tattaaatga atttaatgat gaggatgtca ttggcaatat aaagtcagca atacaacaaa
      241 ttataaaata taattgatgg tgaaatatgc agcggtacta ccaatcggtc aagtattcgc
      301 tgctttgatg atgatgtaaa atgattgttt ttgaactgga tataacagct tggtagggaa
      361 gaaagcataa ccccacaaaa ttcatttacc atcgtctaca cttactcctg taaaccgttc
      421 aggagcagta atgaatctac aacgatttga tgacagcacc cttatccgca tttttgcttt
      481 gcaggagtta caccgtctta aagagcgagg cttaacgcgc ggggcgcttc tcgattatca
      541 cagccgctat aaactcgtct ttctggcgca ttcccagccg gaataccgca aacttggccc
      601 gtttgtggct gatattcatc ggtggcaaaa tctggatgac tattacaacc agtactacca
      661 acgcgtaatt gttttgcttt ctcatcctgc caacccgcga gatcacacca acgttttgat
      721 gcacgttcag ggttattttc gcccgcatat tgattccaca gaacgccagc agctggcagc
      781 gcttatcgac agttatcgcc gtggcgagca accgcttctt gcgccgctga tgcgtatcaa
      841 acactatatg gcgctttatc ctgacgcctg gctttcaggg cagcgttatt tcgaactttg
      901 gccgcgtgtg attaacttgc gccattcagg agttttatga ctacccatct ggtctggttt
      961 cgccaggatt tacgtctgca cgataatctc gcgctggctg ccgcctgccg caattcgtct
     1021 gcacgcgtgc tggcgttgta tatcgctaca ccacgccagt gggcggcgca taatatgtcg
     1081 ccgcgtcagg ctgaacttat caatgctcaa ctgaacgggc tacaaatcgc gctggcggaa
     1141 aaaggtattc ctttattgtt ccgtgaagtg gatgactttg tcgccagcgt cgaattagtt
     1201 aaacaggtgt gcgcggaaaa cagcgttact catctttttt ataactatca gtatgaggtg
     1261 aatgagcggg cgcgggatgt ggacgttgaa agagcgctgc gtaacgtggt gtgtgaagga
     1321 tttgatgaca gcgtgatcct gccgccgggg gcggtgatga ctggcaatca cgagatgtac
     1381 aaagtcttta cgccttttaa gaatgcctgg ctgaaacggc tgcgggaagg gatgccggag
     1441 tgcgtcgctg caccaaaagt tcgtagtagc ggatcgataa agcccgcgcc atccattacg
     1501 ctgaattatc ctcgtcagtc tttcgatact gcgcatttcc cggtggaaga aaaagcggcg
     1561 attgcgcaat tacgccagtt ttgccagaac ggtgccggag agtatgagca acaacgagat
     1621 tttccggcag tggaaggcac cagccgtttg tccgccagcc tggcaacggg cgggttatca
     1681 cctcgccagt gtttgcatcg cctgttggcg gaacagccgc aggcgctgga cggtggggcc
     1741 ggtagtgtct ggcttaatga gctgatctgg cgcgagtttt accgtcacct gataacgtat
     1801 cacccctcgt tgtgtaaaca ttgtccgttt attgcctgga cggagcgtgt gcagtggcag
     1861 agcaatcccg cacatttaca ggcctggcag gaaggcaaaa cgggataccc gattgtcgat
     1921 gctgccatgc gtcagcttaa cagcactggc tggatgcata acaggctacg gatgattaca
     1981 gccagttttc tggtgaaaga tttgttgatc gactggcgcg aaggcgagcg atatttcatg
     2041 tcgcagctga ttgatggtga tttagcagcc aataacggtg gctggcagtg ggcggcttct
     2101 acgggtactg atgcagcgcc gtattttcgt attttcaacc cgacaaccca gggcgagaaa
     2161 tttgaccgcg aaggcgagtt tatccgccag tggctaccgg aactgcgcaa tgtaccgggg
     2221 aaatcggtgc atgagccgtg gaagtgggct gagaaagcag gtgtgaagct ggattatccg
     2281 caaccgatag tcgagcacaa agaggcgaga gtacaaacgt tggcagcgta tgaggcggcg
     2341 cggaagggga aataacagac gcgtcaggca atcgagccca gatgccggat gcggcgtgaa
     2401 cgccttatcc agcctacaaa tttgtgcaaa ttcaataaat tgcaggaaat acgtaggcct
     2461 gataagacgc gtcaagcgtc gcatcaggca tcggtgctgg cctgttaaga ctccagcgcc
     2521 agcgcgcggt ttctgaattt cagcgcctga tacagccaaa tcatcagtac cactcctaca
     2581 cacgccagtg cgccccaggt aatttgatca aatacttcaa tatatgcatt gatggagtag
     2641 ttgatcgccc cggaagcatc aaacgaagcc tgcgatgtct gatcggcaat cacgcccgcc
     2701 agatagttcg caatcgcgcc ggaaagcagc atataaatgc cggttaatac gccggtcaca
     2761 ccggggattt caatgcgcgt aatctgcgac atagcaaccg ggtcgataaa cagttccgca
     2821 aagcccatca ccgccaggcc taataccatc agtggcagag aagagtgacc atacattgcg
     2881 gaccagcggg cgcttaaggt cagaatgcag aatccggcgc tcatcaggcc aaggccaaga
     2941 gcaaattttc cccagatacg cacggtacga ttacccgcga cactctcttt taccacccac
     3001 gccaggaaca caccgcacag cataactgcg aaggcattaa tcgactggaa catcgcggtc
     3061 ggaacggtat aaccaaacat atcgcggtta acgaagcggt cgatataaag gctaatcgag
     3121 ctaccgccct gttgtgcgaa ggcccagaac aacatactga agaaggtgag cgtcacaatc
     3181 agccccagct ccttacgctg tttctggttt tctgctttgc gataaatttt tgccagtaca
     3241 cccaggccaa tgatagtcgc gacaatcaag gcgtataccg accattcttt ccagaacagt
     3301 acggtaatca gcgcaggcgt tgccaccagc agaaccagca gccatcccca gttcggcagc
     3361 agaaagtttg tcgcacgcag tacttttttg ttaacgccgc gggtatgtgt gaaatgacga
     3421 ttgccacata agaaaataac cagacccgcg atcataccca ccgccgccag gccaaagccc
     3481 atcgcccaac tgtactcttc ctgggcgtag ccacaggcaa taggtgcgat aatagacccc
     3541 acgttacccg ccgcatacat cagcgagaaa ccgccatcac gacgcggatc ggttggctca
     3601 tacagctcgc cgagcaggca actgacattg gatttaaaca gaccatagcc acaaacgatt
     3661 atcgccaggg acagatagag gaatgacgga tggatctcac ttgcacccaa caccacatga
     3721 ccgatcgcca tcaataacgc ccccagcatc accgccatgc gattgccgag aactttatcc
     3781 gccaaaaagc caccgaggat tggcgtgaca tacaccagcg aacagtaggc gctaaataac
     3841 tcgtaggcgt gagtatcgtt gtattttagt tgattggtga gatagagaat cagcagggca
     3901 cgcatgccgt aaaagctaaa atattcccag atttgcagcg caacgacata gtaaatagcg
     3961 cgcggttgtg atgcgtgttt attcataata attccacaat tgacggcaac gtcacgtaat
     4021 agttgcgcgg cgtgcttgtg tttccttaaa gttaaaactt tgatacagat ctgattattt
     4081 atgcaatatg ctgtctgatt gcataaatat acattagctg aagcgtgatg atataaacaa
     4141 tttgcgtctt ttggcgtcgg gtttgtttct taatatgtca aaagattggc tatcgacgaa
     4201 caggcgaggt aacgctatgt taacggtgta gctatcagaa tacggaaggc gcgaagatga
     4261 aaaacaccga actggaacaa ctgattaacg aaaaactgaa cagcgcggcg attagtgatt
     4321 acgcgccgaa tggtttgcag gtggaaggca aagagacggt gcaaaaaatt gttaccggtg
     4381 tcactgccag ccaggctttg ctcgatgagg cagtgcgtct gggcgcggat gcggtcattg
     4441 tgcatcatgg ctacttctgg aaaggcgaat ctccggtcat tcgcggcatg aagcgtaacc
     4501 gtttaaaaac gttgctggcg aatgatatca acctgtatgg ctggcatttg ccgcttgacg
     4561 cacatcctga gctgggcaat aacgcacagc tggcggcgtt actggggatc acggtcatgg
     4621 gcgaaattga gccgttggtg ccgtggggcg aactgaccat gcctgtgccg ggactggagc
     4681 tggcttcctg gattgaagcg cgtctgggac gtaagccgtt atggtgtggc gataccggac
     4741 ctgaggtggt ccagcgcgtc gcctggtgca cgggcggcgg gcaaagtttt atcgatagcg
     4801 ccgcgcgttt tggcgtggat gcttttatta ctggcgaagt ttctgaacag accattcatt
     4861 cagcccgcga gcagggattg catttttatg ctgcaggtca ccatgccact gaacgtggtg
     4921 gtattcgcgc attgagcgag tggctgaatg aaaataccga tcttgatgtg acctttattg
     4981 atattcctaa tcctgcataa cgaataatca gagggatcga aagtgcaacg agcgcgttgt
     5041 tatctgatag gtgaaacggc ggtagtgctg gaactggaac cgccggtgac gctggctagc
     5101 cagaaacgga tctggcgact ggcgcagcgt ctggtggata tgccgaatgt ggttgaagcc
     5161 attcccggca tgaacaatat cacggtgatt ttgcgtaatc ctgagtcgct ggcgctggat
     5221 gccatagagc gtttgcaacg ctggtgggag gagagcgagg ccctggaacc tgagtctcgc
     5281 tttattgaaa ttccggtggt ttacggtggt gcaggcggac cggatttggc ggtggtcgcg
     5341 gcgcattgcg ggttgagcga aaaacaggtt gttgaattgc actcctccgt ggaatacgtg
     5401 gtctggtttt taggttttca accgggcttc ccgtatctcg ggagtttgcc ggaacaacta
     5461 cacacgccac ggcgcgctga accgcgcttg ctcgttccgg caggttctgt cgggatcggc
     5521 gggccgcaga ctggtgttta tccgctggca acgccgggtg gctggcagtt gattggtcat
     5581 acctcactca gcctgtttga tccggcgcgt gacgaaccca tcttattacg tccgggagac
     5641 agcgtgcgct ttgtaccgca aaaggaggga gtatgctgaa gattattcgt gcgggcatgt
     5701 ataccactgt gcaagatggc ggtcgtcacg gttttcgcca gtcgggtatc agccactgcg
     5761 gcgcactgga tatgcctgcg ttacgcattg ctaacctgct ggtgggtaat gatgccaatg
     5821 cccccgcgct ggagatcacg ctcggtcagt taacggttga gttcgaaact gatgggtggt
     5881 ttgctctgac gggtgccggt tgcgaagcgc ggctggatga taatgccgtc tggaccggct
     5941 ggcgattgcc gatgaaagca ggccagcgtt taacgcttaa acgcccgcag cacgggatgc
     6001 gcagttatct ggcggtcgcg ggtggtattg atgttccgcc ggtaatgggg tcatgcagca
     6061 ccgatctcaa agtggggatt ggcgggctgg aaggccgttt actgaaggat ggtgaccaac
     6121 tcccgattgg caaatcgaag cgtgatttta tggaagcgca gggcgttaaa cagctgctgt
     6181 ggggcaaccg cattcgcgcc ttgccggggc cggaatatca tgagttcgat cgcgcctcgc
     6241 aggatgcatt ctggcgttcg ccctggcagc ttagcccgca aagtaaccgc atgggctatc
     6301 gcttacaggg gcaaatttta aaacgcacca ccgatcgcga actgttatct cacggtttgt
     6361 taccgggtgt ggtgcaggtg ccgcataacg ggcagcccat tgtgttgatg aacgacgcac
     6421 agaccaccgg tggttacccg cgtattgcct gtatcattga ggctgatatg taccatctgg
     6481 cgcaaattcc gctcggtcag ccgattcatt ttgtccagtg ttcactggaa gaggcactga
     6541 aagcgcggca agatcagcaa cgttatttcg aacaattagc gtggcggctg cacaatgaaa
     6601 attgacctga acgccgatct gggcgaaggc tgcgccagcg acgcagagct attaacgctg
     6661 gtttcctctg ccaatattgc ctgtggattt catgcaggcg atgcgcaaac catgcaggct
     6721 tgcgtgcgtg aagcaataaa aaatggtgtc gcgattggcg ctcatccgag ttttcccgac
     6781 agggaaaatt ttggtcgcag cgctatgcag ctgccgccag aaaccgttta cgcccagacg
     6841 ctgtatcaaa ttggcgcgct ggcaacgatt gcccgtgcgc aaggcggcgt aatgcgtcat
     6901 gtcaaaccgc acggcatgtt gtacaaccag gcggcgaaag aagcacaact ggcagacgcc
     6961 atcgccagag cggtatacgc ttgcgatcca gcattggttc tcgttgggct ggcaggaagc
     7021 gagctgattc gtgcaggcaa gcaatatggc ctgacaacgc gcgaggaagt gtttgccgat
     7081 cgcggttatc aggctgacgg ctcgctggtg ccgcgaagcc agtcaggcgc gttgattgaa
     7141 aacgaagaac aggcgctggc gcaaacgttg gagatggtgc aacacggcag agtcaaaagt
     7201 atcaccggcg aatgggcaac ggtcacggcg caaacggtct gcctgcatgg cgacggcgag
     7261 cacgcactgg cgttcgcccg ccgactacgc tctgcatttg ccgaaaaggg gattgttgtc
     7321 gcagcataac cccgattaat aaagaatgaa aaaaggatat caccatgcct gaaggcccgg
     7381 agatccgccg tgcagcggat aacctggagg cggcgatcaa aggcaaacca ctaactgatg
     7441 tctggtttgc cttcccgcag ttaaaaactt atcaatcaca acttatcggt caacacgtta
     7501 cccatgtgga aacgcgtggt aaggcgttgt taactcattt ttccaacgac ttaacgctct
     7561 acagccataa tcagctttat ggcgtctggc gcgtggttga taccggcgaa gagtcgcaga
     7621 ccacgcgagt attgcgggta aaactgcaaa cggctgacaa aaccattctg ctttatagcg
     7681 cctcggatat tgagatgttg accccggaac aactgaccac gcatccgttt ttacaacgcg
     7741 ttggtcccga tgtgctggat ccgaatctga cgccggaggt ggtgaaagaa cggttattgt
     7801 cgccgcgctt tcgtaaccgt cagtttgctg gattactgct cgatcaggcg tttctggctg
     7861 ggcttggcaa ttatttgcgt gtggagatcc tctggcaggt tgggttgact ggaaatcata
     7921 aagcgaaaga cctcaatgcg gcgcaactgg atgcactcgc acacgcgtta ctggatattc
     7981 ctcggttttc ttacgctacg cgggggcagg tggatgagaa taagcatcat ggggcgctgt
     8041 ttcgctttaa ggtttttcat cgtgatggcg aactgtgcga acgctgtggc ggcatcattg
     8101 agaaaaccac gctgtcatct cgcccgtttt actggtgtcc gggctgccag cactaggccg
     8161 tacgtttcgg cgcataggtt gaaataaacc gcgcaatggc aggccctgtc agcaaaatac
     8221 tgaacaggcg cagggtttgc atcgccataa tgagcgccat atcggcattg ctccctgcgg
     8281 cgatgaccgc cacggtatca agcccgccgg ggctggtggc gaggtaggca gtcataaaat
     8341 caatgtgcat aaaccgggtc agcccccacg ccatacccgc acaaatagcc agcagagcaa
     8401 aaatcgacag caggatttgt ggtagcgggc gtagtgcccg cagtaagatt tgtttatcga
     8461 aaccaagacc aatccgccag ccaattgcca tatacgccat cgccagcagc cattccggta
     8521 gttcgatggt gatgagctga ccagactgga gcactgcgcc cgccagcatc gggatgagca
     8581 tcgtgccgga aggaagacgc agcaggcatc ccactgtacc ggcaacgacc gcgagcaaaa
     8641 tagtaagcag gagattaatg ctcactggcg ggaaccagac gatatgctgg ttaaccgctt
     8701 cagcgttatc gcccagcatc attcgcgtga ccagaactgc ggcccccgcg acaaacagca
     8761 ctcgcagata ctgcataaac gccaccaggc ggatatctgc gccgtaatcc tgcgccatcg
     8821 cgaccattgc cgccgccccg ccaggcgagg agccccaggc accagtattt ccgggcagtg
     8881 agctatagcg caccagtagc catcccacga tagcgctgga aagcaacgtc accagtaaaa
     8941 tagctaacac aatcggccag ttaaccgcca gcgtggtcag gatcgaaccg gtcaagtttt
     9001 gcgcaatcat gcagccaaga atggcctgcg cggcgagaaa agcggagcgg gggagttgca
     9061 ggttaacccc gcgcatactg aagatgatcc cggcgatcat cggcccaagt aataatgccg
     9121 ccgggagatg taccgcgagg aaaccaatag aaaggagaag tgatagcaca cataacattc
     9181 cccactgcaa aactggcatc ctttgctccc tcattattta ctctttggtg tatcgataca
     9241 gcataaagaa tgccgggcat ggaaaaaata acggtaatca ataaaagccg gaaaattggc
     9301 aattaaccga agaagccctc tccggctgga aagggcaata ggctaaatca gttgtagtta
     9361 attttgaaaa gtaccactgt atcaaaggga ccaacgtcga tgggcgcacc cggcgtggat
     9421 gacaattctg ccgtgtaggt tttttcatac actaacaggt cgctggtaaa atcggcatac
     9481 tcactgtatt tgttgaaagt atagggtgag gcattttcat ccaacaatcg aagcttcagg
     9541 ccattgccaa tgagcagtga tttatcttcg tcgataaggg gttcatcggt ataaaacgaa
     9601 gagctgactt taaaaccgtc ggtacactgg tcgttttgtg atttggttgt agttatttta
     9661 aattgctgcg acatcgtatg gcgacgaata tccagcacgt taaatcggcc aaaatcaacg
     9721 acctgggttt caggtgagat agcaaagttg acgctgcaat ccagcacgcg gatattttcc
     9781 agaccggtaa tgtgatattt cagattcttc gcggtagggt cttcgttcac gctgcctgcg
     9841 ccatcaaact gtacgacgat gtaatcgctg agtttgctct gataatcatg gggcggcatg
     9901 gcgcgaactt taacgtaaag ccgcatacgc gccacaaacg tccgcgattt gtgcataacg
     9961 gttggatcgc cggagcaaag tttattttgc cagcccatcg caatgatctg ctccggggtg
    10021 taaatatcaa tattcttact gtcgatgcac tgattcgtgt cgatgcggct tttcccaagg
    10081 cttgcgtcat agtccacgcc ttcataggtt acgcccaatt gataataggg atcctgaata
    10141 ccggggtaag ggtttaccca ggcgtagacg tgctcgcttt caaaattacc gtttgtgttg
    10201 ttgtcacagt agaccggaat ttttatatcg tcagattccc agattttatc gccgggtttg
    10261 gaattggccg gaacggcgaa gggcataata gcttccgatt tttctacagc gccgtttgcg
    10321 gtaccaaaat agcagttcag cgccagcgcc gcttttagcg gcaaaaaaat cagacagatg
    10381 gcgagcaaca cgcctttggc gaatctcatt ttttattctc caccggctgc aggcggcatt
    10441 gccgctggca ggagagcgtg ttcatacgca atgcgccgta gtcgtccatg tagccaagat
    10501 aaaactgctt gccgctatag ctgcccgtgc tggtggtgac cgaactgaac ggtgcgacca
    10561 tcgtgctttt aaagccaggc agcacgcctt tctcatcccg cccaagatag gcaatagtca
    10621 gatagtatcc ggtcgggttt ttcagggtaa gctggttatt ctgctggcta acctgtagct
    10681 gttgctcaac atgatcgccc atttttttgc gtaatgcggc gggtcgccag aatagcttaa
    10741 tgcgactttg gacggcgact tgcaggatcg catggccctc gctgccttcc gggacaggcg
    10801 gaatttcacg caggttatag taaaagagag attcgcgatc ggtgggtaat ttcgcggtgg
    10861 cggcttgttt catgatccga acctgcgaag ccgcttttgg ctccagtcgc tggataggcg
    10921 gcagggcgac gagaaattca tcgcttttct ctcccttttc attttctatc catgaatagg
    10981 cgaggtaggg gagtttatcg ctgcggtttt ccacgcgcag gctggttgct ttatctttgc
    11041 tattaaagat gatgcgcgtg cggtcaggct gcactgcgcc gtggctgcac atactggcaa
    11101 ccagaagaat caaagggaat cctttaacaa atgtcatcgt cttttcctta attaatgaca
    11161 gggtaaaatc aggcgcttag tcacatcttc cagatgttcc ggcagatgaa tggcacacgt
    11221 tttctggtcg ccccagtgca cggtaaattg ttgattttca tcgaccccac tcagccatgc
    11281 gtgaccgttt tcgccaacca tcccgacgct gacaccgcct tcagcctggc gaacatcggc
    11341 accgagcggc gggaaatgcc cgttagcatc gctgataatg acgttgagat ctttcccggc
    11401 gcgggacgcc agcgatttaa agccgatggc gccttcggtc caggtttctt tcaccacgtt
    11461 ttcggaaacg gtaacgccgt caggcaagtc gttcatattg accgccaccg tcgtaggctg
    11521 gtagctggaa acaaacggta ccacggcaat accaaagcgg ttggtgtagt caatattgcc
    11581 ctgaatcgga atatcgccca cgccgtcggt gctgaccatc aggcgtggtt cattggtgga
    11641 actgcggcga tggaatgccg cgccatgttg agtcgcggta aacgagccgc tccaactggc
    11701 actggcggaa gtgtaatcat tggcggcata ggttccggtc aaatcccatt cccccgtcga
    11761 actgaggtgt tggtaattgc cgctgacctg cgcaccgtta tccgggcgat cggattgtat
    11821 tccagccgac attccccaac tgttgcgttc atccagggta tcgttccacg acatgcggtg
    11881 ggtggtacta ttactgttgt tctgcatgtc ataacccagt cgaccacttt ccccaatcgg
    11941 taaggagatc gaaaagtaaa tctggttgtc gcgatcttta tcttcataat gggtggtgtt
    12001 gaacgaggtc gagacagaaa tatctttcca gtcgccaatg tcgacgttaa aaccggcggt
    12061 aatgttggcc gtggtggagg tatccgcatt ccaccagctc tgatgcagga tgttggcgta
    12121 gagattaaga ttcagcggag taataggctg accgaaggag aggctgatgg tttgcttttc
    12181 atcctgcgcg tcggcgtcat tgtatttgtg atcaatgtag ttagcgtagc tgtgaaaatg
    12241 gcgatcggag aagcgatagg ctgccaatga gatggtgctg ttggtcgcat caacctgttt
    12301 gctgtagtta aagcggtagc tgtaaccctg ctcatcctgt tgagtatcaa aatggctgtc
    12361 ggcccaggtc acgtcaaacg acagcgcccc cagccagagc atattttgcc caatacccag
    12421 cgcgccggaa cgatagtcat cgcctgcgag aagcatgccg ccatatagtg aagtgttgga
    12481 gagcatcccc caggagactt catggctaat gaaggtttca tcctcggtgt ggtgcgacat
    12541 agaggaacgc gggcgaccgg cagccagttt atagcgcacc tggccctgac gcgtcaggaa
    12601 cggggtagag gccgctgaaa cctggaagtt attcacccgt ccatcttctt cgctcacttt
    12661 gacatccagt gtaccttgca ctgactgatt caggtcatca ataataaatg ggcctggcgg
    12721 aacttttttc tggtaaatca cgcgaccaga atggcttatc gtgaccgtgg cgttagtctg
    12781 tgcgatgccg ctgatttgcg gcgcgtagcc gcgtaactcc caggggagca ttcgatcgtc
    12841 gctggctaac gccgcaccgg tataggagaa accatcaaaa atgttagagc taaaatcggt
    12901 ttcgcccagg gttaatcggg aacctatttg cggtaacggg cggaacaggt aggtccgtga
    12961 aatcgccccg gattgttcac ggttgtcgcc gctatcggac tggctaagct gataatcgct
    13021 gcgcagacgc catgccccgg cgttgaggcc cgtggtgcca taggcgttca ggttgttgct
    13081 gctgctgccg ctttgtgggc gataagtgct ggcaaacagg ttgtaatcca tcaggaagcc
    13141 aggaataccg ttattccacg ttgagggtgg cgtccagttt tctgaatgcc acgccagcca
    13201 tgcctgcggg attgtaatat tgagctgttg atttgcctga tcgaaagtaa agatgatttc
    13261 agggaagaca ctaaaatcga cacattcttt tattgccggt aattttttac gaaagtcaga
    13321 tttaagtccg aagagttcga tcagcgattc attgatacaa ggaataattt tatcgccaga
    13381 tttttgccag cgaatttgtt gcccattact gattttatta ttattaaccg taacactaac
    13441 aaagtaatcg ccgggagcta cgacgccttt ttgattgagc aacgatatat caacgctatc
    13501 ccgaatcgac ttatcgagaa cgttaaggtt aaattcagct gccagcgcag gaggggttac
    13561 cattgccaga caggaaagaa cagagagtcg ataaatattc acggcatcca tacctgaaca
    13621 atattcgcaa aaggcggcta atcagccgcc ttaaaaagga ttattggtaa tccagaacgt
    13681 aagttgcgtt agcagtcacg gcccccgcgg taaccgaaac tgcgttatcc atctgttcca
    13741 tccaggcaaa gaagtttaac gtttgttctg ttgaacttgc caataagtca attgttggcg
    13801 cttctgttcc caatacaaca aatgattcat ctttgttcat gagacgtacg ccaacgccgg
    13861 tagcctcgcc agcactgatg ttactaagga gcggcgttgc acctgtcgtt ttagctgtgc
    13921 tgtcgaaggt cacgcctacc tttgagacca cgccaccgtt gccatcatcc cagcctgcaa
    13981 tatcacagtt gattaagcga atattgacag gttttttctc gctatgatgt tctcgattta
    14041 tgtgggatgt ggttacttca cccaggtcaa tgttcatatc aaggtcatca gggtgaattt
    14101 cacaaggtgc ttcaataact tcacctttaa agtggatttc accagatcct tcatctgcag
    14161 caaaaacagg tgaagcgaat agtagagata tggccagggc ggccagtgtt ttttgtcctt
    14221 taaacataca tgaatccttc atgggctatg ataaagtata ctaattattc cgacagaata
    14281 gtggtttttt gttcatgtaa taatattaat gttgctacat tatgtttgag agtttctaaa
    14341 agctctcgaa tcataaataa agtgaattaa tcctattacg agtttattta attgtgggtg
    14401 ctacccaatg tattctacat cgattggcgg gataacggtg gtggtgaatg aaaaattagg
    14461 attttattta gttatcggat atgtattctt gtaacgtgta attatgtatt tgtgtccgat
    14521 tacgtttttt tgtcgataga cataactaac gaacaaaaaa aagcccgcca gatattcagg
    14581 cgggctaaac gatatatggt tcaaatcaga taattaatgt ttaacatctg atttaaagtc
    14641 tcgtttttca tagccggtgt acaactggcg cggacgggcg atcttcatgc cgtcactgtg
    14701 catttcgctc cagtgagcga tccagccaac ggtacgggcc atagcgaaga tgacggtgaa
    14761 catggaagac ggaataccca tcgctttcag gatgatacca gagtagaaat cgacgttcgg
    14821 gtacagtttc ttctcgataa agtacgggtc gttcagcgcg atgttttcca gctccatagc
    14881 cacttccagc aggtcatctt tggtgcccag ctctttcaga acttcatggc aggtttcacg
    14941 cattacggtg gcgcgcgggt cgtaattttt gtacacgcgg tgaccgaagc ccatcaggcg
    15001 gaaagaatca tttttatctt tcgcacgacg aacaaattcc ggaatgtgtt taacggagct
    15061 aatttcttcc agcattttca gcgccgcttc gttagcacca ccgtgcgcag gtccccacag
    15121 tgaagcaata cctgctgcga tacaggcaaa cgggttcgca cccgaagagc cagcggtacg
    15181 cacggtggag gtagaggcgt tctgttcatg gtcagcgtgc aggatcagaa tacggtccat
    15241 agcacgttcc agaatcggat taacttcata cggttcgcac ggcgtggaga acatcatatt
    15301 caggaagtta ccggcatagg agagatcgtt gcgcggataa acaaatggct gaccaatgga
    15361 atacttgtaa cacatcgctg ccatggtcgg cattttcgac agcaggcgga acgcggcaat
    15421 ttcacgatga cgaggattgt taacatccag cgagtcgtga tagaacgccg ccagcgcgcc
    15481 ggtaatacca cacatgactg ccattggatg tgagtcgcga cggaaagcgt ggaacagacg
    15541 ggtaatctgc tcgtggatca tggtatgacg ggtcaccgta gttttaaatt cgtcatactg
    15601 ttcctgagtc ggtttttcac cattcagcag gatgtaacaa acttccaggt agttagaatc
    15661 ggtcgccagc tgatcgatcg ggaaaccgcg gtgcagcaaa ataccttcat caccatcaat
    15721 aaaagtaatt ttagattcgc aggatgcggt tgaagtgaag ccagggtcaa aggtgaacac
    15781 accttttgaa ccgagagtac ggatatcaat aacatcttga cccagcgtgc ctttcagcac
    15841 atccagttca acagctgtgt ccccgttgag ggtgagtttt gcttttgtat cagccattta
    15901 aggtctcctt agcgccttat tgcgtaagac tgccggaact taaatttgcc ttcgcacatc
    15961 aacctggctt tacccgtttg ttatttggct cgccgctctg tgaaagaggg gaaaacctgg
    16021 gtacagagct ctgggcgctt gcaggtaaag gatccattga tgacgaataa atggcgaatc
    16081 aagtacttag caatccgaat tattaaactt gtctaccact aataactgtc ccgaatgaat
    16141 tggtcaatac ttccacactg ttacataagt taatcttagg tgaaataccg acttcataac
    16201 ttttacgcat tatatgcttt tcctggtaat gtttgtaaca actttgttga atgattgtca
    16261 aattagatga ttaaaaatta aataaatgtt gttatcgtga cctggatcac tgttcaggat
    16321 aaaacccgac aaactatatg taggttaatt gtaatgattt tgtgaacggc ctatactgcc
    16381 gccaggtctc cggaacaccc tgcaatcccg agccacccag cgttgtaacg tgtcgttttc
    16441 gcatctggaa gcagtgtttt gcatgacgcg cagttataga aaggacgctg tctgacccgc
    16501 aagcagaccg gaggaaggaa atcccgacgt ctccaggtaa cagaaagtta acctctgtgc
    16561 ccgtagtccc cagggaataa taagaacagc atgtgggcgt tattcatgat aagaaatgtg
    16621 aaaaaacaaa gacctgttaa tctggaccta cagaccatcc ggttccccat cacggcgata
    16681 gcgtccattc tccatcgcgt ttccggtgtg atcacctttg ttgcagtggg catcctgctg
    16741 tggcttctgg gtaccagcct ctcttcccct gaaggtttcg agcaagcttc cgcgattatg
    16801 ggcagcttct tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc
    16861 gtcgtaggta ttcgccacat gatgatggat tttggctatc tggaagaaac attcgaagcg
    16921 ggtaaacgct ccgccaaaat ctcctttgtt attactgtcg tgctttcact tctcgcagga
    16981 gtcctcgtat ggtaagcaac gcctccgcat taggacgcaa tggcgtacat gatttcatcc
    17041 tcgttcgcgc taccgctatc gtcctgacgc tctacatcat ttatatggtc ggttttttcg
    17101 ctaccagtgg cgagctgaca tatgaagtct ggatcggttt cttcgcctct gcgttcacca
    17161 aagtgttcac cctgctggcg ctgttttcta tcttgatcca tgcctggatc ggcatgtggc
    17221 aggtgttgac cgactacgtt aaaccgctgg ctttgcgcct gatgctgcaa ctggtgattg
    17281 tcgttgcact ggtggtttac gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa
    17341 attgccagtc agagaatttg atgcagttgt gattggtgcc ggcggcgcag gtatgcgcgc
    17401 ggcgctgcaa atttcccaga gcggccagac ctgtgcgctg ctctctaaag tcttcccgac
    17461 ccgttcccat accgtttctg cgcaaggcgg cattaccgtt gcgctgggta atacccatga
    17521 agataactgg gaatggcata tgtacgacac cgtgaaaggg tcggactata tcggtgacca
    17581 ggacgcgatt gaatatatgt gtaaaaccgg gccggaagcg attctggaac tcgaacacat
    17641 gggcctgccg ttctcgcgtc tcgatgatgg tcgtatctat caacgtccgt ttggcggtca
    17701 gtcgaaaaac ttcggcggcg agcaggcggc acgcactgcg gcagcggctg accgtaccgg
    17761 tcacgcactg ttgcacacgc tttatcagca gaacctgaaa aaccacacca ccattttctc
    17821 cgagtggtat gcgctggatc tggtgaaaaa ccaggatggc gcggtggtgg gttgtaccgc
    17881 actgtgcatc gaaaccggtg aagtggttta ttttaaagcc cgcgctaccg tgctggcgac
    17941 tggcggagca gggcgtattt atcagtccac cactaacgcc cacattaaca ctggcgacgg
    18001 tgtcggcatg gctatccgcg caggcgtacc ggtgcaggat atggaaatgt ggcaattcca
    18061 cccgaccggc attgccggtg cgggcgtact ggtcaccgaa ggttgccgtg gtgaaggcgg
    18121 ttatctgctg aacaaacatg gcgaacgctt tatggagcgt tatgcgccga acgccaaaga
    18181 cctggcgggc cgtgatgtgg tggcgcgttc catcatgatt gaaatccgtg aaggtcgcgg
    18241 ctgtgatggt ccgtgggggc cacacgcgaa actgaaactc gatcacttgg gtaaagaagt
    18301 tctcgaatcc cgtctgccgg gtatcctgga gctttcccgt accttcgctc acgttgatcc
    18361 ggtgaaagag ccgattccgg ttatcccaac ctgtcactac atgatgggcg gtattccgac
    18421 caaagttacc ggtcaggcgc tgactgtgaa tgagaaaggc gaagatgtgg ttgttccggg
    18481 gctgttcgcg gtaggtgaaa ttgcctgcgt atcggtacac ggtgctaacc gtctgggcgg
    18541 caactcgctg ctggacctgg tggtctttgg tcgcgcagcg ggtctgcatc tgcaagagtc
    18601 tatcgccgag cagggcgcat tgcgcgatgc cagcgagtct gatgttgaag catctctgga
    18661 tcgtctgaac cgctggaaca ataatcgtaa cggtgaagat ccggtggcga ttcgtaaagc
    18721 gctgcaagaa tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa
    18781 agggcttgag cagttgaaag ttatccgcga gcgtctgaaa aatgcccgtc tggatgacac
    18841 ttccagcgag ttcaacaccc agcgcgttga gtgtctggaa ctggataacc tgatggaaac
    18901 ggcgtatgca acggctgttt ctgccaactt ccgtaccgaa agccgtggcg cgcatagccg
    18961 cttcgacttc ccggatcgtg atgatgaaaa ctggctgtgc cactccctgt atctgccaga
    19021 gtcggaatcc atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc cggcattccc
    19081 gccgaagatt cgtacttact aatgcggaga caggaaaatg agactcgagt tttcaattta
    19141 tcgctataac ccggatgttg atgatgctcc gcgtatgcag gattacaccc tggaagcgga
    19201 agaaggtcgc gacatgatgc tgctggatgc gcttatccag ctgaaagaga aagatcccag
    19261 cctgtcgttc cgccgctcct gccgtgaagg tgtgtgcggt tccgacggtc tgaacatgaa
    19321 cggcaagaat ggtctggcct gtattacccc gatttcggca ctcaaccagc cgggcaagaa
    19381 gattgtgatt cgcccgctgc caggtttacc ggtgatccgc gatttggtgg tagacatggg
    19441 acaattctat gcgcaatatg agaaaattaa gccttacctg ttgaataatg gacaaaatcc
    19501 gccagctcgc gagcatttac agatgccaga gcagcgcgaa aaactcgacg ggctgtatga
    19561 atgtattctc tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga atcccgataa
    19621 gtttatcggc ccggcaggct tgttagcggc atatcgtttc ctgatcgata gccgtgatac
    19681 cgagactgac agccgcctcg acggtttgag cgatgcattc agtgtattcc gctgtcacag
    19741 catcatgaac tgcgtcagtg tatgtccgaa gggactgaac ccgacgcgcg ccatcggtca
    19801 tatcaagtcg atgttgttgc aacgtaatgc gtaaaccgta ggcctgataa gacgcgcaag
    19861 cgtcgcatca ggcaaccagt gccggatgcg gcgtgaacgc cttatccggc ctacaagtca
    19921 ttacccgtag gcctgataag cgcagcgcat caggcgtaac aaagaaatgc aggaaatctt
    19981 taaaaactgc ccctgacact aagacagttt ttaaaggttc cttcgcgagc cactacgtag
    20041 acaagagctc gcaagtgaac cccggcacgc acatcactgt gcgtggtagt atccacggcg
    20101 aagtaagcat aaaaaagatg cttaagggat cacgatgcag aacagcgctt tgaaagcctg
    20161 gttggactct tcttacctct ctggcgcaaa ccagagctgg atagaacagc tctatgaaga
    20221 cttcttaacc gatcctgact cggttgacgc taactggcgt tcgacgttcc agcagttacc
    20281 tggtacggga gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt tccgccgcct
    20341 ggcgaaagac gcttcacgtt actcttcaac gatctccgac cctgacacca atgtgaagca
    20401 ggttaaagtc ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc atgcgaatct
    20461 cgatccgctg ggactgtggc agcaagataa agtggccgat ctggatccgt ctttccacga
    20521 tctgaccgaa gcagacttcc aggagacctt caacgtcggt tcatttgcca gcggcaaaga
    20581 aaccatgaaa ctcggcgagc tgctggaagc cctcaagcaa acctactgtg gcccgattgg
    20641 tgccgagtat atgcacatca ccagcaccga agaaaaacgc tggatccaac agcgtatcga
    20701 gtctggtcgc gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg aactgaccgc
    20761 cgctgaaggc cttgaacgtt acctcggtgc aaaattccct ggtgcaaaac gcttctcgct
    20821 ggaaggcggt gacgcgttaa tcccgatgct taaagagatg atccgccacg ctggcaacag
    20881 cggcacccgg gaagtggttc tcgggatggc gcaccgtggt cgtctgaacg tgctggtgaa
    20941 cgtgctgggt aaaaaaccgc aagacttgtt cgacgagttt gccggtaaac ataaagaaca
    21001 cctcggcacg ggtgacgtga aataccacat gggcttctcg tctgacttcc agaccgatgg
    21061 cggcctggtg cacctggcgc tggcgtttaa cccgtctcac cttgagattg taagcccggt
    21121 agttatcggc tctgttcgtg cccgtctgga cagacttgat gagccgagca gcaacaaagt
    21181 gctgccaatc accatccacg gtgacgccgc agtgaccggg cagggcgtgg ttcaggaaac
    21241 tctgaacatg tcgaaagcgc gtggttatga agttggcggt acggtacgta tcgttatcaa
    21301 caaccaggtt ggtttcacca cctctaatcc gctggatgcc cgttctacac cgtactgtac
    21361 tgatatcggt aagatggttc aggcaccgat tttccacgtt aacgcggatg atccggaagc
    21421 cgttgccttt gtgacccgtc tggcgctcga tttccgtaac acctttaaac gtgatgtctt
    21481 catcgacctg gtgtgctacc gccgtcacgg ccacaacgaa gccgacgagc cgagcgcaac
    21541 ccagccgctg atgtatcaga aaatcaaaaa acatccgaca ccgcgcaaaa tctacgctga
    21601 caagctggag caggaaaaag tggcgacgct ggaagatgcc accgagatgg ttaacctgta
    21661 ccgcgatgcg ctggatgctg gcgattgcgt agtggcagag tggcgtccga tgaacatgca
    21721 ctctttcacc tggtcgccgt atctcaacca cgaatgggac gaagagtacc cgaacaaagt
    21781 tgagatgaag cgcctgcagg agctggcgaa acgcatcagc acggtgccgg aagcggttga
    21841 aatgcagtct cgcgttgcca aaatttatgg cgatcgccag gcgatggctg ccggtgagaa
    21901 actgttcgac tggggcggcg cggaaaacct cgcttacgcc acgctggttg acgaaggcat
    21961 tccggttcgc ctgtcgggtg aagactccgg tcgcggtaca ttcttccacc gccacgcggt
    22021 gatccacaac cagtctaacg gttccactta cacgccgctg caacacatcc ataacggcca
    22081 gggcgcgttc cgtgtctggg actccgtact ttctgaagaa gcagtgctgg cgtttgaata
    22141 cggttatgcc accgcagaac cacgcactct gactatctgg gaagcgcagt tcggtgactt
    22201 cgccaacggt gcgcaggtgg ttatcgacca gttcatctcc tctggcgaac agaaatgggg
    22261 ccggatgtgt gggctggtga tgttgctgcc gcacggttac gaagggcagg ggccggagca
    22321 ctcctccgcg cgtctggaac gttatctgca actttgtgct gagcaaaaca tgcaggtgtg
    22381 cgtaccgtct accccggcac aggtttacca catgctgcgt cgtcaggcgc tgcgcgggat
    22441 gcgtcgtccg ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc tggcggtttc
    22501 cagcctcgaa gaactggcga acggcacctt cctgccagcc atcggtgaaa tcgacgagct
    22561 tgatccgaag ggcgtgaagc gcgtagtgat gtgttctggt aaggtttatt acgacctgct
    22621 ggaacaacgt cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg agcaactcta
    22681 cccgttcccg cataaagcga tgcaggaagt gttgcagcag tttgctcacg tcaaggattt
    22741 tgtctggtgc caggaagagc cgctcaacca gggcgcatgg tactgcagcc agcatcattt
    22801 ccgtgaagtg attccgtttg gggcttctct gcgttatgca ggccgcccgg cctccgcctc
    22861 tccggcggta gggtatatgt ccgttcacca gaaacagcaa caagatctgg ttaatgacgc
    22921 gctgaacgtc gaataaataa aggatacaca atgagtagcg tagatattct ggtccctgac
    22981 ctgcctgaat ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc cggcgacgca
    23041 gtcgtacgtg atgaagtgct ggtagaaatc gaaactgaca aagtggtact ggaagtaccg
    23101 gcatcagcag acggcattct ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct
    23161 cgtcagatcc ttggtcgcct gcgtgaaggc aacagcgccg gtaaagaaac cagcgccaaa
    23221 tctgaagaga aagcgtccac tccggcgcaa cgccagcagg cgtctctgga agagcaaaac
    23281 aacgatgcgt taagcccagc gatccgtcgc ctgctggctg aacacaacct cgacgccagc
    23341 gccattaaag gcaccggtgt gggtggtcgt ctgactcgtg aagatgtgga aaaacatctg
    23401 gcgaaagccc cggcgaaaga gtctgctccg gcagcggctg ctccggcggc gcaaccggct
    23461 ctggctgcac gtagcgaaaa acgtgtcccg atgactcgcc tgcgtaagcg tgtggcagag
    23521 cgtctgctgg aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga agtcaacatg
    23581 aagccgatta tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc
    23641 cgtctgggct ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa acgttacccg
    23701 gaagtgaatg cttctatcga cggcgatgac gtggtttacc acaactattt cgacgtcagc
    23761 atggcggttt ctacgccgcg cggcctggtg acgccggttc tgcgtgatgt cgataccctc
    23821 ggcatggcag acatcgagaa gaaaatcaaa gagctggcag ttaaaggccg tgacggcaag
    23881 ctgacggttg aagatctgac cggtggtaac ttcaccatca ccaacggtgg tgtgttcggt
    23941 tccctgatgt ctacgccgat catcaacccg ccgcagagcg caattctggg tatgcacgct
    24001 atcaaagatc gtccgatggc ggtgaatggt caggttgaga tcctgccgat gatgtacctg
    24061 gcgctgtcct acgatcaccg tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg
    24121 atcaaagagt tgctggaaga tccgacgcgt ctgctactgg acgtgtagta gtttaagttt
    24181 cacctgcact gtagaccgga taagacatta tcgccttctc cggcaattga agcctgatgc
    24241 gacgcttaac gcgtcttatc aggcctacgg gaagcaccga tgtaggtcgg ataaggcgct
    24301 cgcgccgcat ccgacaagcg atgccagatg cgacgcttac acgtcttatc aggcctacgg
    24361 gtgaccgaca atacccggga gcgatacgaa atattcggtc tacggtttaa aagataacga
    24421 ttactgaagg atggacagaa cacatgaact tacatgaata tcaggcaaaa caactgtttg
    24481 cccgctatgg cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag
    24541 aagccgcttc aaaaatcggt gccggtccgt gggtagtgaa atgtcaggtt cacgctggtg
    24601 gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg
    24661 cagaaaactg gctcggcaag cgtctggtaa cgtatcaaac agatgctaat ggccaaccgg
    24721 ttaaccagat tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg
    24781 ttgtagaccg tagttcccgt cgcgtggtct ttatggcctc taccgaaggc ggcgtggaaa
    24841 tcgaaaaagt ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga
    24901 ctggcccgat gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac
    24961 tggttcagca gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc
    25021 tggcgttgat cgaaatcaac ccgctggtca tcaccaaaca gggtgatctg atttgcctcg
    25081 acggcaaact gggcgctgat ggcaacgccc tgttccgcca gcctgatctg cgcgaaatgc
    25141 gtgaccagtc gcaggaagat ccacgtgaag cacaggctgc acagtgggaa ctgaactacg
    25201 ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga
    25261 tggacatcgt taaactgcac ggcggagaac cggctaactt cctggacgtt ggcggcggcg
    25321 caaccaaaga acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag
    25381 ccgttctggt taacatcttc ggcggtatcg ttcgttgcga tctgatcgct gacggtatca
    25441 tcggcgcggt agcggaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca
    25501 acgccgaact cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag
    25561 gtctgacgga tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt
    25621 aattgataaa aacaccaagg ttatctgcca gggctttacc ggtagccagg ggactttcca
    25681 ctctgaacag gccattgcat acggcaccaa aatggttggt ggcgtaactc caggtaaagg
    25741 cggtactacc cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac
    25801 tggcgctacc gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga
    25861 agccatcgac gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga
    25921 tatgctgact gtgaaagtga aactggatga agccggcgtt cgtatgatcg gcccgaactg
    25981 cccaggcgtt atcaccccgg gtgaatgcaa aattggtatc caaccgggtc acattcacaa
    26041 accgggtaaa gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca
    26101 gaccacggat tacggtttcg gtcagtcgac ctgtgtcggt atcggcggtg acccgatccc
    26161 tggctctaac tttatcgaca ttctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat
    26221 cgtgatgatc ggtgagatcg gcggtagcgc tgaagaagaa gctgctgcgt acatcaaaga
    26281 gcacgttacc aagccagttg tgggttacat cgctggtgtg actgcgccga aaggcaaacg
    26341 tatgggccac gcgggtgcca tcattgccgg tgggaaaggg actgcggatg agaaattcgc
    26401 tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact
    26461 gaaaactgtt ctgaaataaa tacttgtaat aagaaatagc cctcgccgct tccctctaca
    26521 ggaatggcga agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt
    26581 gccttaaacg ttaaccacgg tctaaatctg actgcaggct tgactgcatg accacagtca
    26641 ctgtggattg ccgcaaccga ccgcaagcca cgaataagaa cgacggcgaa tttcgaatcg
    26701 ttacctgatg cactggcttc ccagcgtttg accgtagcgt taagtaaatc aacaaaagcg
    26761 atgttcaccg cttttcgtca agtcgtgcca tttgtcagcc aggatcgggt ctttaccccg
    26821 gatattgcag catcgcaaaa attcttagag ggatatgcta taaatc
//
LOCUS       DAZGBC010000058        26632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGBC010000058 DAZGBC010000000
VERSION     DAZGBC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.058"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(336..2909)
                     /gene="clpB"
                     /locus_tag="ACHK1J_002341"
     CDS             complement(336..2909)
                     /gene="clpB"
                     /locus_tag="ACHK1J_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO7911594.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3039..3770)
                     /gene="yfiH"
                     /locus_tag="ACHK1J_002342"
     CDS             complement(3039..3770)
                     /gene="yfiH"
                     /locus_tag="ACHK1J_002342"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO7911595.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3767..4747)
                     /gene="rluD"
                     /locus_tag="ACHK1J_002343"
     CDS             complement(3767..4747)
                     /gene="rluD"
                     /locus_tag="ACHK1J_002343"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO7911596.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEAMRADFEEHKDEVDWL"
     gene            4882..5619
                     /gene="bamD"
                     /locus_tag="ACHK1J_002344"
     CDS             4882..5619
                     /gene="bamD"
                     /locus_tag="ACHK1J_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO7911597.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5890..6231
                     /gene="raiA"
                     /locus_tag="ACHK1J_002345"
     CDS             5890..6231
                     /gene="raiA"
                     /locus_tag="ACHK1J_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO7911598.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6334..6459
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6335..6382
                     /gene="pheL"
                     /locus_tag="ACHK1J_002346"
     CDS             6335..6382
                     /gene="pheL"
                     /locus_tag="ACHK1J_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO7911599.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6481..7641
                     /gene="pheA"
                     /locus_tag="ACHK1J_002347"
     CDS             6481..7641
                     /gene="pheA"
                     /locus_tag="ACHK1J_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO7911600.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7684..8805)
                     /gene="tyrA"
                     /locus_tag="ACHK1J_002348"
     CDS             complement(7684..8805)
                     /gene="tyrA"
                     /locus_tag="ACHK1J_002348"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO7911601.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8816..9886)
                     /gene="aroF"
                     /locus_tag="ACHK1J_002349"
     CDS             complement(8816..9886)
                     /gene="aroF"
                     /locus_tag="ACHK1J_002349"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO7911602.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10096..10461
                     /gene="yfiL"
                     /locus_tag="ACHK1J_002350"
     CDS             10096..10461
                     /gene="yfiL"
                     /locus_tag="ACHK1J_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO7911603.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10611..11129
                     /gene="yfiR"
                     /locus_tag="ACHK1J_002351"
     CDS             10611..11129
                     /gene="yfiR"
                     /locus_tag="ACHK1J_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO7911604.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11119..12345
                     /gene="dgcN"
                     /locus_tag="ACHK1J_002352"
     CDS             11119..12345
                     /gene="dgcN"
                     /locus_tag="ACHK1J_002352"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO7911605.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12361..12843
                     /gene="yfiB"
                     /locus_tag="ACHK1J_002353"
     CDS             12361..12843
                     /gene="yfiB"
                     /locus_tag="ACHK1J_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7911606.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12920..13267)
                     /gene="rplS"
                     /locus_tag="ACHK1J_002354"
     CDS             complement(12920..13267)
                     /gene="rplS"
                     /locus_tag="ACHK1J_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO7911607.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13309..14076)
                     /gene="trmD"
                     /locus_tag="ACHK1J_002355"
     CDS             complement(13309..14076)
                     /gene="trmD"
                     /locus_tag="ACHK1J_002355"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO7911608.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14107..14655)
                     /gene="rimM"
                     /locus_tag="ACHK1J_002356"
     CDS             complement(14107..14655)
                     /gene="rimM"
                     /locus_tag="ACHK1J_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO7911609.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14674..14922)
                     /gene="rpsP"
                     /locus_tag="ACHK1J_002357"
     CDS             complement(14674..14922)
                     /gene="rpsP"
                     /locus_tag="ACHK1J_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO7911610.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15059..16420)
                     /gene="ffh"
                     /locus_tag="ACHK1J_002358"
     CDS             complement(15059..16420)
                     /gene="ffh"
                     /locus_tag="ACHK1J_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO7911611.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16587..17378
                     /gene="ypjD"
                     /locus_tag="ACHK1J_002359"
     CDS             16587..17378
                     /gene="ypjD"
                     /locus_tag="ACHK1J_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO7911612.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17399..18685
                     /gene="yfjD"
                     /locus_tag="ACHK1J_002360"
     CDS             17399..18685
                     /gene="yfjD"
                     /locus_tag="ACHK1J_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO7911613.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSILLAPLQILMMPLVWLLNAITRMLMRIMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18740..19333)
                     /gene="grpE"
                     /locus_tag="ACHK1J_002361"
     CDS             complement(18740..19333)
                     /gene="grpE"
                     /locus_tag="ACHK1J_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO7911614.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19456..20334
                     /gene="nadK"
                     /locus_tag="ACHK1J_002362"
     CDS             19456..20334
                     /gene="nadK"
                     /locus_tag="ACHK1J_002362"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO7911615.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNILSTKLGWSKKLF"
     gene            20420..22081
                     /gene="recN"
                     /locus_tag="ACHK1J_002363"
     CDS             20420..22081
                     /gene="recN"
                     /locus_tag="ACHK1J_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO7911616.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22230..22571
                     /gene="bamE"
                     /locus_tag="ACHK1J_002364"
     CDS             22230..22571
                     /gene="bamE"
                     /locus_tag="ACHK1J_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO7911617.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22633..22923)
                     /gene="yfjF"
                     /locus_tag="ACHK1J_002365"
     CDS             complement(22633..22923)
                     /gene="yfjF"
                     /locus_tag="ACHK1J_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO7911618.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22913..23350)
                     /gene="ratA"
                     /locus_tag="ACHK1J_002366"
     CDS             complement(22913..23350)
                     /gene="ratA"
                     /locus_tag="ACHK1J_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO7911619.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23521..24003
                     /gene="smpB"
                     /locus_tag="ACHK1J_002367"
     CDS             23521..24003
                     /gene="smpB"
                     /locus_tag="ACHK1J_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO7911620.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24218..24580
                     /gene="ssrA"
                     /locus_tag="ACHK1J_002368"
     tmRNA           24218..24580
                     /gene="ssrA"
                     /locus_tag="ACHK1J_002368"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24764..25957
                     /locus_tag="ACHK1J_002369"
                     /pseudo
     CDS             24764..25957
                     /locus_tag="ACHK1J_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101258.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
     gene            complement(25960..>26632)
                     /locus_tag="ACHK1J_002370"
     CDS             complement(25960..>26632)
                     /locus_tag="ACHK1J_002370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO7911621.1"
                     /translation="AEEISDETQNAIKTAYSEVQINGRLSTLRSRILLSSKKCPLCGI
                     ESSTDLDHYLPESIYKCLAVYSRNLIPTCHKCNNKKRAGVAENGVGFIHVYYFDEPDG
                     VFFVANTEMIDGALVIDFNINMIEGMTREVYDSLCFQNRVVNLNERLKMEANDFLGGL
                     YTSLEETYNSAGQEGLRLFLKRTRDDYTRRYGLNHWRRALIDSLSDNVEFLDAGFITA
                     LGTPE"
BASE COUNT         6825 a         6976 c         6210 g         6621 t
ORIGIN      
        1 gcccacacag attgtctgat aaattgttaa agagcagtgc cgcttcgctt tttctcagcg
       61 gtacggggtg tgcataatac gctttcccgc tacagagtca agcatttatt tttgctttct
      121 ctgccggaat tctcaggaga accccgctga cccggcggcg tgtttgccgt tgttccgtgt
      181 cagtggtggc gcattatagg gagttattcc ggcctgacaa gcgaaaaata taaaaacttt
      241 atcgttcgct cacttttcag gcaaaacatc ttaaatatag tcttttccgt ctaacttata
      301 gacaaaaacg agccccgaag ggctcgtttt atcatttact ggacggcgac aatccggtct
      361 tcattaactt ccaggcgaat cactttaccc ggaaccaatt caccagacag tatttgctgt
      421 gccagcgggt tttcgatctg ctgctgaatt gcacgtttca gaggacgtgc accatagacc
      481 ggatcgtaac cgttctcgct cagcagtttc agcgcctcgt cagaaatgtg gatttcataa
      541 ccacgttctt ccagacgttt atacagacgt ttcaactgaa tctgcgcaat cgacgcaatg
      601 tgctgttcac ccagcggatg gaagaccacc acttcatcga tacggttaat gaattccgga
      661 cggaagttat ggctaaccac accgagcacc agctctttca tgtgcgcata atccagttca
      721 ccgaagcgtt cctgaatcaa atcggaaccg aggttagagg tcataatgac aaccgtatta
      781 cggaagtcga ccgttctccc ctgcccgtca gtcagacgcc catcatccag tacctgcaac
      841 agaatgttga agacatccgg atgcgctttt tccacttcat ccagcaggat aacggaatac
      901 ggacgacgac gcacagcttc ggtcaggtag ccaccttctt cataaccgac atatcccgga
      961 ggcgcaccaa ccagacgaga caccgagtgt ttctccataa actcggacat atcgatacgg
     1021 accatcgcct cgtcgctatc aaacataaag ttcgccagcg ccttacaaag ctctgttttc
     1081 cccacaccag ttgggccgag gaacaggaat gaaccaatcg ggcgatttgg atccgccagc
     1141 cccgcacggc tacgacgaat agcgttagat accgcatcaa ccgcttcgtt ctgaccaatt
     1201 acgcgatggt gcagttcttg ctccatacgc agcagttttt cgcgctcgct ttccatcatg
     1261 cgagaaaccg gaatccccgt ccaacgcgcc agcacttcag caatttcggc gtcggtcact
     1321 ttattacgca atagacgcat agttttgcct tcgagctgcg ttgcggcttc cagttgcttt
     1381 tccagttccg ggattttgcc gtattgcagt tcagacatcc gcgccaggtc ccccacacgg
     1441 cgagcctgtt caatagcgat tttcgcctgt tccagttccg ctttaatggt ctgcgtacca
     1501 gaaagcgatg ccttctctgc tttccactct tcttctaact cggagtactg acgttctttg
     1561 tcgctcagtt cttcgttgag catatccagg cgttttttac tggcttcatc agactctttc
     1621 attaacgcct gttgttccag tttgagctgg atgatacgac gatcgagtcg gtcgagttct
     1681 tctggttttg agtcaatttg catacgaatg ctggatgctg cttcatcgat caggtcgata
     1741 gctttatccg gcagctgacg gtcagcaatg tagcgatgag acaacgtcgc cgctgcaaca
     1801 attgccgggt cagtaatttg cacatggtgg tgcaattcgt aacgttcttt caggccacgc
     1861 agaatcgcaa tggtatcttc aacagaaggc tcggcaacaa acactttctg gaaacgacgt
     1921 tccagcgcag catctttttc gatgtactgg cgatattcgt caagcgtagt ggcacctacg
     1981 cagtgcaatt caccacgcgc cagcgccggt ttcagcatgt ttccggcgtc cattgcgcca
     2041 tcggctttac ccgcgccgac catggtatgt aattcgtcga taaacaggat gacgttgcct
     2101 tcctgtttgg caagatcgtt aagcacgcct tttaaacgtt cttcaaactc accgcgatat
     2161 ttcgccccag ccaccagcgc gcccatatcc agcgccagta cccggcggcc tttcaaccct
     2221 tccggcactt cgccgttgat aatacgctgc gccagacctt caacgatggc agttttaccg
     2281 acgccgggtt caccaatcag taccgggtta tttttagtac gacgttgcag cacctgaatg
     2341 gtacggcgaa tttcttcatc acgaccaatc accggatcga gtttgccctg ttcggctcgt
     2401 tcggtaaggt cgatggtata ttttttcaaa gcctgacgtt ggtcttcagc accttgatcg
     2461 ttcacgcttt cacctccacg catttgttca atcgcttgag taatgttggc ggtggtcgcc
     2521 cctgctgctt tcaggatgtc ggccagcgtg ccgcgagact caagtgccgc cagaacgaac
     2581 agttctgacg agataaagtt atcaccacgt ttttgcgcca gcttgtcgca aagattaaga
     2641 acgcgcacca gatcctgtga tggctggaca tcaccaccag taccttcaac ctgcggtaaa
     2701 cgatttaatg cctgattgat atctgtgcgc aactggccag catttatgcc agctgatgtt
     2761 aacaaaggac taaccgaccc cccttcctga ttcagcaggg cgctcattaa atgaagtggt
     2821 tcgatgaatt ggttgtcgtg cccgagtgca agtgattggg catcggcaag agcaagctgg
     2881 aatttattag taagacgatc cagacgcata actcctccca taacggatca aagttgctac
     2941 tggagattaa atgaggtcat ccctcaatta ttcaaggtta ttgaccagat taatgtgaaa
     3001 agaaaatcac gcgtaccgga tcgtcttgat tctttaggtt atatcagcca aatgaaactt
     3061 gccatacgcc cggtggtctt gtcgcgacga taagagaaga aagtctcatt ttccgtatat
     3121 gtacaacggt cgccgccgaa aatttgttca acaccaacgc tcgccagacg ctgccgggca
     3181 agcagataaa tatccgccag atacttatca ccatgctgaa tgaaagctgt actcgctttg
     3241 gcgtctgctg ccataaacgc ctcgcgaacc tccgccccca cttcgaacgc gcgtggacca
     3301 attgccggcc ctaaccaggc gagaatattt tccggattat cagcaaaaca ggaaactgtc
     3361 tcttccagca cgcctgcgca cagtccacgc cagccagcat gagcggcggc gacttccgtt
     3421 cccgctcgat tgcaaaacag cacagggagg cagtcggcag tcatcactgc gcaaaccgtg
     3481 ccaggcgtat tgctataaga ggcatctgct cgttttgagg cataaggttc gccagtaagc
     3541 ttaagcacat ctttgccgtg tacctgctca agccagaccg gtttagaagg caaattgccc
     3601 gcagcaaaaa gtcgcttgcg attctcctca acatgatccg ggttatcgcc acaatgggca
     3661 ccgaggttga gcgagtcata cgggggcaag ctcacgccgc cgatacgagt agagctacaa
     3721 gccgcaacac cttttggcag cggccactgc gggacaatca gcttactcat aaccagtcca
     3781 cttcatcctt atgttcttcg aaatcggcac gcatcgcctc aatcagctcc accatatctt
     3841 gtggaatagg cgcatgccat tccatttcga tgccggagat cggatgataa agacgcagca
     3901 tggttgcatg aagcgcctgg cggtcaaact tacgcagcgt ggagataaac gcttccgaag
     3961 caccttttgg cggacgcgga cggccaccat aaaccggatc gcccaccagc ggatgagtga
     4021 tatgggccat atgcacgcgg atctggtgcg tacgtccagt ttccagacgc aaccgcagac
     4081 gcgtgtgcac acggaagtgt tccatgatgc gatagtgagt caccgctggt ttgcccatcg
     4141 gatgcaccgc catgtgagta cgtttggtcg ggtggcgact gattggctca tccaccgtac
     4201 cgccagcggt catatgacca atcgccaccg cttcgtactc acgagtaatt tcacgccttt
     4261 gcaaagattc gactaaacgc gtctgagccg gaacggtttt tgccacaacc atcaggccag
     4321 tggtgtcttt atccagacga tggacgatgc ccgcacgcgg tacatcggca ataggcggat
     4381 aataatgaag caacgcattc agtaccgtgc catccgggtt acccgcgcca ggatgaacca
     4441 ccaggtcgcg cggtttatta atgacgatga tgtcttcatc ttcatagacg atatccagcg
     4501 ggatatcctg cggttcaaaa cgcgcttctt cttcaatctc tgcgttgatg gcaacctgct
     4561 cgccacccaa tactttttct ttcggcttat cacaaagttt gccgttaacc agcacgcgct
     4621 ggtcgaggat ccattctttt attcgcgaac gtgaataatc cgggaacatt tcggccaaag
     4681 cctgatctaa gcgttgaccg agttggtttt cggacaccgt tgcagtgagc tgtactcgtt
     4741 gtgccatata tactgcttct tcgtttatcg ttggggtttt acggctttgc cgtttaatat
     4801 agtgtgctat tgtagctggt cttaaccggg agcaggaaca gagaatctcc cgtattacat
     4861 tttgaggaaa gtcaaaacgt catgacgcgc atgaaatatc tggtggcagc cgccacacta
     4921 agcctgtttt tggcgggttg ctcggggtca aaggaagaag tacctgataa tccgccaaat
     4981 gaaatttacg cgactgcaca acaaaagctg caggacggta actggagaca ggcaataacg
     5041 caactggaag cgttagataa tcgctatccg tttggtccgt attcgcagca ggtgcagctg
     5101 gatctcatct acgcctacta taaaaacgcc gatttgccgt tagcgcaggc tgccatcgat
     5161 cgttttattc gccttaaccc gacccatccg aatatcgatt atgtcatgta catgcgtggc
     5221 ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt tctttggcgt tgaccgtagc
     5281 gatcgagatc ctcaacatgc acgagctgcg tttagtgact tttccaaact ggtgcgcggc
     5341 tatccaaaca gtcagtacac caccgatgcc accaaacgtc tggtattcct gaaagatcgt
     5401 ctggcgaaat atgaatactc cgtggccgag tactatacag aacgtggcgc atgggttgcc
     5461 gtcgttaacc gcgtagaagg catgttgcgc gactacccgg atacccaggc tacgcgtgat
     5521 gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga tgaatgcgca agctgaaaaa
     5581 gtagcgaaaa tcatcgccgc aaacagcagc aatacataac agaaacctga aacacaaaac
     5641 ggcagccctt gagctgccgt ttttttattc tgtcagttgt gaaactgaag cgatttagtc
     5701 actatcgatc tcatcaaata tggctcgctt tgagatattc ctcaagtaaa aaaacacctc
     5761 ttcctgcgat ttctcacaaa aaagattcgt tgacaaaaag tgacaaaatt atgagatttc
     5821 catcacacat tttgacatca ggaacggtat gctgaattca ccaagacggg aagacaagag
     5881 gtaaaattta tgacaatgaa cattaccagc aaacaaatgg aaattactcc ggccatccgc
     5941 caacatgtcg cagaccgtct cgccaaactg gaaaaatggc aaacacatct gattaatcca
     6001 catatcattc tgtccaaaga gccacaaggg tttgttgctg acgccacaat caatacacct
     6061 aacggcgttc tggttgccag tggtaaacat gaagatatgt acaccgcaat taacgaattg
     6121 atcaacaagc tggaacggca gctcaataaa ctgcagcaca aaggcgaagc acgtcgtgcc
     6181 gcaacatcgg tgaaagacgc caacttcgtc gaagaagttg aagaagagta gtcctttata
     6241 ttgagtgtat cgccaacgcg ccttcgggcg cgttttttgt tgacagcgtg aaaacagtac
     6301 gggtactgta ctaaagtcac ttaaggaaac aaacatgaaa catacaccgt ttttcttcgc
     6361 attctttttt accttcccct gaatgggagg cgtttcgtcg tgtgaaacag aatgcgaaga
     6421 cgaacaataa ggcctcccaa atcggggggc cttttttatt gataacaaaa aggcaacact
     6481 atgacatcgg aaaacccgtt actggcgctg cgagagaaaa tcagcgcgct ggatgaaaaa
     6541 ttattagcat tactcgcaga gcggcgcgaa ctggccgtcg aggtgggaaa agccaaactg
     6601 ctctcgcatc gcccggtacg tgatattgat cgtgaacgcg atttactgga aagattaatt
     6661 acgctcggta aagcgcacca tctggacgcc cattacatta ctcgcctgtt ccagctcatc
     6721 attgaagatt ccgtattaac tcagcaggct ttgctccaac aacatctcaa taaaattaat
     6781 ccgcactcag cacgcatcgc ttttctcggc cccaaaggtt cttattccca tcttgcggcg
     6841 cgccagtatg ctgcgcgtca ctttgagcaa ttcattgaaa gtggctgcgc caaatttgcc
     6901 gatattttta atcaggtgga aaccggccag gccgactatg ccgtcgtacc gattgaaaat
     6961 accagctccg gtgccataaa tgacgtttac gatctgctgc aacataccag cttgtcgatt
     7021 gttggcgaga tgacgttaac tatcgaccat tgtttgttag tctccggcac gactgattta
     7081 tccaccatca atacggtcta cagccatccg cagccattcc agcaatgcag caaattcctt
     7141 aatcgttatc cgcactggaa gattgaatat accgaaagta cgtctgcggc aatggaaaag
     7201 gttgcacagg caaaatcacc gcatgttgct gcgttgggaa gcgaagctgg cggcactttg
     7261 tacggtttgc aggtactgga gcggattgaa gcgaatcagc gacaaaactt cacccgattt
     7321 gtggtgttgg cgcgtaaagc cattaacgtg tctgatcagg ttccggcgaa aacgacgttg
     7381 ttaatggcga ccgggcaaca agccggtgcg ctggttgaag cgttgctggt actgcgcaac
     7441 cacaatctga ttatgacccg tctggaatca cgcccgattc acggtaatcc atgggaagag
     7501 atgttttatc tggatattca ggccaatctt gaatcagcgg aaatgcaaaa agcattgaaa
     7561 gagttagggg aaatcacccg ttcaatgaag gtattgggct gttacccaag tgagaacgta
     7621 gtgcctgttg atccaacctg atgaaaaggt gccggatgat gtgaatcatc cggcactgga
     7681 ttattactgg cgattgtcat tcgcctgacg caataacacg cggctttcac tctgaaaacg
     7741 ctgtgcgtaa tcgccgaacc agtgctccac cttgcggaaa ctgtcaataa acgcctgctt
     7801 atcgccctgc tccagcaact caatcgcctc gccgaaacgc ttatagtaac gtttgattaa
     7861 cgccagatta cgctctgacg acataatgat gtcggcataa agctgcggat cctgagcaaa
     7921 cagtcgcccg accatcgcca gctcaaggcg gtaaatcggc gaagagagcg ccagaagttg
     7981 ctcaagctga acattttctt ctgccaggtg cagcccgtaa gcaaaagtag caaagtggcg
     8041 cagtgcctga ataaacgcca tattctgatc gtgctcgaca gcgctaatac gatgcaaccg
     8101 agcgccccag acctgaattt gctccagaaa ccattggtat gcttccggct tacgtccatc
     8161 acaccagacc acaacttgct ttgccaggct accgctgtcc gggccgaaca tcgggtgtaa
     8221 ccccagtacc gggccatcgt gcgccgccag catggcctgt aatggtccat ttttcactga
     8281 tgccagatca accagaatac aatctttcgg taaaggcggt aatttgccga taacttgctc
     8341 agtaacgtgg attggcacac taacaatcac cattccggca tcggcaacaa tatcagccgc
     8401 tcgatcccag tcatgttgct ccagaatccg cacctgataa cccgatagtg tcagcatctt
     8461 ctcgaacagg cgtcccatct gaccgccgcc gccaacgata actaccgggc gtaacgcagg
     8521 acaaagtgtt ttaaatcctt tgtcgttttc actggagtaa gattcacgca tcacccgacg
     8581 caaaacatcc tcaatcagat ctggcggtac acccagagct tccgcctctg cacgacgcga
     8641 ggccaacata gatgcctcgc gctccggaac ataaataggc agtccaaagc ggcttttcac
     8701 ctcgcccact tcagcaacca gttccagacg cttcgctaat aaattcagca gcgctttatc
     8761 gacttcatca atttgatcgc gtaatgcggt caattcagca accataataa acctcttaag
     8821 ccacgcgagc cgtcagctgc ccgttcagat cctgatgaat ttcacgcagc aaggcatcgg
     8881 tcatttccca gctaatgcag gcatcggtta cggatacacc gtatttcatt tcactgcgcg
     8941 gttgctcgga agactgattg ccctcgtgga tattactttc gatcatcaga ccaataattg
     9001 agcgattgcc atctttgatt tgagcaacca cggattctgc caccgcaggc tgacggcgat
     9061 aatctttatt ggaattaccg tggctgcaat ctaccatcag agacgggcgc agtcccgcct
     9121 gttccatctc tttttcacat tgcgcaacat ccgcagggct atagttcggc gctttaccac
     9181 cgcgcaggat cacatgtccg tccggattcc cctgagtttg tagcaacgca acctgacctg
     9241 cctggttaat gccaacaaaa cggtgcggct gggcggcggc gcgcatagcg ttaattgctg
     9301 ttgccagact gccgtcggtg ccgtttttaa aaccaaccgg catggaaagc ccggaggcca
     9361 tttcacggtg agtttgcgat tccgttgtac gagcaccaat tgctgaccag ctaaacaaat
     9421 cgcccaggta ttgcgggcta ttcggatcta acgcttccgt cgccagtggc agtcccatat
     9481 tcaccagctc aagcagcaat ttacgcgcga tctgcagccc ggcttctaca tcaaaagagc
     9541 catccatatg gggatcgtta attaaccctt tccagccgac agtggtacgg ggtttttcaa
     9601 aatagacgcg cattaccaga tagaggctat cgctgacctc tgcggcaagg gctttaaatc
     9661 gacgagcata ttccagagca gtttccggat catgaatgga acaaggacca catactacca
     9721 gcagacgagg atcgcgcccg gcgataatat ctgaaatggt tttacgcgag tcagcaatct
     9781 gggcttcttg ttgcaggctt aatggaaaag cggccttcag ttgtgccgga gtcattaaaa
     9841 cctgttcgtc ggtaatatgt acgttattca gcgcgtcttt ttgcatgatg gcgatcctgt
     9901 ttatgctcgt ttgcgatagt tgatcctcag cgaggatgac gtaacgataa cacataaagt
     9961 aaagttttca atccatattt cgtacatttt tctttacaga agcaatttat tcgcgctttc
    10021 aacccttgat tctgtataga taaatttaca ccacctttga aaacaatccg ctatgctttg
    10081 aaaaaggaga aagaaatgat gaaaaagttt atcgccccct tgttggcttt actggttagc
    10141 ggatgccaga ttgatcctta tactcacgcg ccaaccttga ccagcaccga ctggtatgat
    10201 gttggtatgg aagatgcgat atcgggcagc gccataaaag atgacgatgc atttagcgat
    10261 tcacaggcgg atcgcggtct ataccttaaa ggatatgccg aaggacaaaa gaaaacttgc
    10321 cagaccgatt ttacttatgc ccgaggactt tccggtaaaa gctttcctgc cagctgcaac
    10381 aatgttgaaa atgccagcca actgcatgaa gtatggcaaa aaggggctga tgaaaatgcc
    10441 agcgcaatac gattaaatta attaaaaccc cacaaataaa ttaaacataa gattttactc
    10501 attatgtttt atttctcaca atgatgacga ccgcatgaaa taatgatatt ttaatgagca
    10561 ttctgaaaaa atatcgatgc atttcgagcg aagatggtgg ggatccctga atgcgttttt
    10621 ctcaccgact gttcttactg ctgattctcc tgctgacggg agcaccaata ctcgctcagg
    10681 agccttctga cgttgcgaaa aacgtgcgta tgatggtttc cggtattgtc agttacaccc
    10741 gctggcctgc gctatcaggg ccaccaaagt tatgtatctt ttcatcttca cgttttagta
    10801 ccgccctaca agagaatgcc gcgacatctt tgccttatct gcctgtcatc attcacactc
    10861 aacaagaggc gatgatttca ggctgcaatg gtttttattt tggcaacgag tcaccgacat
    10921 tccagatgga attaaccgaa caatatccgt caaaggcgtt attattaatt gccgaacaaa
    10981 ataccgagtg cattattggt agcgcctttt gtctgatcat ccacaataat gacgtcagat
    11041 ttgccgtaaa cctggatgcc ttatcgcgta gcggtgtaaa ggtcaacccg gatgtcttaa
    11101 tgctcgcacg gaagaaaaat gatggataac gataattctc ttaataagcg ccccacgttt
    11161 aaaagagcat tacgcaacat cagtatgacc agcatattta tcactatgat gctgatctgg
    11221 ttgctgcttt ccgtgacctc ggtgctgacc ctgaaacagt acgcgcaaaa aaacctggca
    11281 ctgacagcag caacaatgac ttacagtctg gaagcagctg tcgtttttgc cgatggccct
    11341 gcagcaactg aaacactggc agcgctgggc cagcaagggc aattttcaac tgcagaagta
    11401 cgtgataagc agcaaaatat tctggcatcc tggcattaca cccgtaagga tccaggcgat
    11461 actttcagca atttcataag ccactggctc ttccctgccc ccatcattca gccgattcgt
    11521 cacaatggtg aaaccattgg cgaagtacgc ttaaccgctc gcgacagttc aatcagccat
    11581 tttatctggt tttcgctcgc cgtactgacc ggttgtattc tgctggcatc aggaatcgca
    11641 attaccctca cccgccattt gcacaatggc ctggtggaag cactgaaaaa tatcaccgat
    11701 gtcgtacatg atgtgcgttc caaccgcaat ttttcccgac gagtttcgga agaacgtatc
    11761 gctgagtttc accgcttcgc tctcgacttc aacagtctgc tggatgaaat ggaagagtgg
    11821 cagcttcgtt tacaggccaa aaatgcgcag cttctgcgta ccgcgctaca tgacccatta
    11881 accgggctgg ctaaccgcgc agcgtttcgt agcggcatca atacgttgat gaacaattcc
    11941 gatgcccgaa aaacgtcggc gttactattt cttgatggcg ataatttcaa atatatcaat
    12001 gatacctggg gtcatgcgac gggcgataga gtcttgattg aaatcgcaaa acggttagct
    12061 gaatttggcg ggctgcgaca taaagcatac cgcctgggcg gcgatgaatt cgctatggtg
    12121 ctctatgatg tacagtcgga atctgaagtg cagcagatat gctcagcact gacacaaatc
    12181 tttaatctcc cgtttgatct tcataatggc catcagacca ccatgacatt aagcattggt
    12241 tacgcaatga ccattgcgca cgcttctgcg gaaaaattac aagagctggc cgatcacaat
    12301 atgtatcagg ccaaacacca gcgtgccgaa aagctggtga gataacaagg atatatcgat
    12361 atgataaagc acctggtagc acccctgatt ttcacctcac taatactgac tggctgccag
    12421 tcccctcagg gaaagtttac tcctgagcaa gtcgccgcta tgcaatctta tggatttact
    12481 gaatccgccg gcgactggtc gctgggctta tcagatgcca ttctgttcgc aaaaaatgac
    12541 tacaaattgc tcccggaaag ccagcaacag atccaaacca tggcagccaa attggcctcg
    12601 acagggctaa cacatgcccg tatggatgga cacaccgata actatggtga agacagttac
    12661 aacgaaggct tatcattgaa acgggcgaat gtcgtggccg atgcatgggc tatgggtgga
    12721 caaattccac gcagcaatct caccacacag ggtttaggaa aaaaatatcc catagccagt
    12781 aacaagaccg cccagggccg cgccgagaac cgccgcgtcg cagtggtgat tactacccct
    12841 taaaacacga gcaaacagat aaaaaaaggg ccagccaatt ggccagccct tcttaacagg
    12901 atgtcgctta agcgaaatct tagttaagac gctctttgat acgagcagcc ttaccagtac
    12961 gctcacgcag gtagtacagt ttagctttac gaacagcacc acgacgtttg acagaaatgc
    13021 tgtcaactac cggagagtga gtctggaaga cacgctcaac gccttcgccg ttggaaattt
    13081 tacgaacagt gaatgcagag tgcagaccgc ggttacgaat agcgataacc acgccctcga
    13141 atgcctgcag acgttttttg gaaccttcaa caacccatac tttcacttcc acggtatcac
    13201 ccggacggaa ggaaggtacg tcctgcttca tctgctcttg ttcaagttgc ttaataatgt
    13261 tgctcataat ttaatctctt atcctgggta aactgatatc tcgggggctt acgccatccc
    13321 atcatgttta tgttgctgtt gtgcgtgttc cgttttgaac tccgccagca accttgcttg
    13381 ctcttcagtc agagccaggt tttccagaag ttcaggtctt ctaagccagg tacggcccag
    13441 cgactgtttc aaacgccagc gacgtatctc ggcatggttg cccgacagta acactggcgg
    13501 aacctccatc ccttctaaca cctcaggccg cgtatagtgc gggcaatcca gcaatccttc
    13561 agcaaaggaa tcttccgttg ccgaggcttc atgtcccagt actcccggaa taaaccggga
    13621 aacggagtca atcagcgtca ttgctggtaa ctcaccacca ctgagaacgt aatcgccgat
    13681 tgaccattct tcgtcaattt cggtttggat cacgcgctca tctatacctt cgtagcgacc
    13741 gcacaccaga atcaattttt gattcgttgc cagttcgctg acgcccgctt gatcaagctt
    13801 gcgtccctgt ggtgaaagat aaatcacctt tgcgccttca cccgccgcgg cttttgctgc
    13861 atgaatggcg tcccgcaagg gttgcaccat cattaacatc cccggtccgc cgccgtaagg
    13921 acgatcgtcc acggtacggt gccggtcatg cgtgaaatcg cgaggactcc agctctggat
    13981 gctcagcagg ccatttttaa ctgcccggcc agttaccccg taatcggtaa ttgcgcggaa
    14041 catttcagga aacaggctaa ttatgccaat ccacatagcg ccgtctttta ccgtttatcc
    14101 ggtggtttaa aaaccaggat cccaatctac ttcgattgaa cgagtagtga gatcgacttt
    14161 cttgataacc tgcccatcga ggaacggtac gagacgttcc ttgataccaa acgcatcttt
    14221 caggtttgcc ttaatgacga gaacgtcatt ggatccggtt tccatcatat cgacgacttt
    14281 accgagatcg tagccttcag tggttactac ctggcagccc atcaggtctt tccagtagta
    14341 gtcaccctct tcaagctgag gcagctgcga tgaatccacg acaatttcac aatttgtcag
    14401 caggttcgcc gcatcacgat cgtcaacgcc tttcagcttg atgatcatgt cctgattgtg
    14461 gtgcttccag ctttccagct ggacttgctg ccactgaccc gccttctgga taaaccaggg
    14521 ctgatagtca aaaatgcttt cggcgtcttc ggtggaagaa aacactctga gccacccacg
    14581 aataccgtaa gacgaaccca tttttcccaa aacgatggga tcaacaggtg cttgcgcggt
    14641 gagttgtttg ctcatcatga ccaccgtgac agattaagct gctttgttta cttctttgat
    14701 cagcgcagca acgcgatcag aaatagttgc gccctggcca acccagtgag cgatgcgatc
    14761 cagatccagg cgagtgcctt cttctttttc gctagcgatt gggttgaaga aaccaacgcg
    14821 ctcgatgaag cgaccgttgc gtgcattacg gctgtcagcg acaacaacct ggtagaacgg
    14881 acgcttttta gcgccgtgac gtgctaaacg aatagttacc ataacatcct cttgtgtgaa
    14941 taaaacaacc ggaccccatc gaggaacgga gtccggtgtc atattaaaag cccgaaaatt
    15001 ttactgattt ctggagaaaa tgcaatcagc agttaataac tctgtcgcat aaggtcattt
    15061 agcgaccagg aaatcccggc ggcatcatcc ccttcatgct tctcatcatc ttcgccattc
    15121 cgcccttctt cattttcttc atcatgcgct gcatgtcgtc gaactgtttc agaagacggt
    15181 taacgtcctg cacctgcata ccgcaaccgg cagcaatacg gcgtttacgc gaacctttga
    15241 tgatttctgg cttagcgcgc tctttcatcg tcatcgagtt gatgatggct tccatacgca
    15301 ccagcacttt atcgtccatc tgtgacttga cgttatccgg gatctgcccc atgcccggca
    15361 gcttgcccat cagactagcc atgccgccca tatttttcat ctggcgcagt tgctcaagaa
    15421 agtcgttgag atcgaagccg tcgccttttt ttagcttgct ggctaatttc tctgcctgcg
    15481 cgcggtcaac tttgctttcg atatcttcga tcagcgacag tacgtcgccc atgccgagaa
    15541 tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc tcgccaacac
    15601 cgaggaactt gatcggtttg ccagtgatgt gacgaataga gagcgccgca ccgccgcggg
    15661 catcgccgtc cactttggtc aacactacgc cggtcagcgg taacgcttca ttgaatgctt
    15721 ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaacagg gtttcaaccg
    15781 gatttatgga cgcatggact tgtttgatct cgtccatcat cgcttcgtca acgtgcagac
    15841 gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct ttcagcgccg
    15901 cgttaacgat atctaccggc ttctgaccaa catcagaagg gaagaaatca acgcccacct
    15961 gctctgcaag cgtctcaagc tgtttgattg ccgctggacg ataaacgtcg gcagaaacca
    16021 ccagcacttt tttcttgtgc ttctcgcgca ggaacttacc gagcttaccg acactggtgg
    16081 ttttaccggc accttgcagg cccgccatca gcacgaccgc aggcggttgt gccgccagat
    16141 tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggacg attttgacga
    16201 actcctgccc cggcgtcagg cttttattaa cttcatgacc aaccgctttc tcttttacgc
    16261 gattgataaa ctcacgcact accggcagag cgacgtccgc ctccagcagc gccatgcgca
    16321 cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca ctgatattgc
    16381 gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc ctggggtgga
    16441 aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa catgaaggcg
    16501 tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg cttctctttc
    16561 ttattgctca aactgtcgac attactatgc ccgtttttgc tctgctcgcg cttgtcgcct
    16621 actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc ggctggcggc
    16681 gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct ctggaagccc
    16741 gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt ggttcattgg
    16801 tcagtttgat gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt ggctggttgc
    16861 tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcaacc ttcatgccca
    16921 atgaatacat cacccatctg gaagctacac ctgggatgct ggtgcacatt ggcttatcgc
    16981 tcttttccta tgccacgcta attatcgccg ccctgtacgc gctgcaactg gcgtggattg
    17041 attaccaact gaagaacaag aagctggcgt ttaaccagga aatgccgcca ttgatgagta
    17101 tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt gctgctaacg ctcacgcttt
    17161 gcactggcct gttctacatg cacaacttat ttagcatgga aaatatcgac aaggctgtgc
    17221 tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattat catgaaggat
    17281 ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggtgc agtcattctg acactggcct
    17341 acttcggcag ccgaattgtc cagcagttaa tcagctaaac ccagaaaagg agtttcccct
    17401 ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg tcatttcagc
    17461 ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc tgcgacatat
    17521 ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta agccagaccg
    17581 cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct ccgcgctcgg
    17641 cactattgtt gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga ctggtgtgct
    17701 gacctttgtg gtgctggttt ttgccgaagt gttgccgaaa actatcgctg cgctataccc
    17761 ggaaaaagtc gcttatccga gtagtattct gttagcaccg ctgcaaattt tgatgatgcc
    17821 gctggtctgg ttgctgaacg ctatcacccg catgttaatg cgcattatgg gcatcaaaac
    17881 cgatatcgtg gtcagcggct ctttgagcaa agaagagttg cgcactatcg tgcacgaatc
    17941 gcgctcacaa atttcccgtc gcaatcagga tatgctgctg tcggtgctcg atctggaaaa
    18001 aatgaccgtt gatgacatca tggtgccgcg cagtgaaatt atcggtattg atatcaacga
    18061 tgactggaaa tctattctgc gccaactctc ccactcacct cacgggcgca tcgtgctcta
    18121 ccgtgattcg ctggacgacg ccatcagtat gctgcgagta cgtgaagcct ggcggttgat
    18181 gtcggagaaa aaagagttca ccaaagaaac catgctgcgc gccgcggacg agatctattt
    18241 tgtgccggaa ggtacgccgc tcagcacgca gttggtaaag tttcagcgca acaaaaagaa
    18301 agtcggcctg gtcgtcaacg agtatggaga cattcaaggg ctggtgacgg ttgaagatat
    18361 tctggaagag attgtcggcg atttcactac gtcgatgtcg ccaacacttg ccgaagaggt
    18421 cacgccgcaa aacgacggtt cggtgattat cgatggcacc gccaacgtgc gggaaatcaa
    18481 caaagccttt aactggcatc taccggaaga tgatgcccgc acggttaatg gcgtcattct
    18541 tgaggcactg gaagagatcc ctgtcgcagg cacccgcgtg cgtattggcg agtacgatat
    18601 agatattctc gacgtacagg acaatatgat taagcaggta aaagtttttc ctgtgaaacc
    18661 gctgcgcgag agtgtggcgg agtaacgaaa acggccctgc attcgcatgc agggccgtga
    18721 attattacga aaacagaaat tacgcttttg ctttcgctac agttaccatc gccgcacgaa
    18781 tcgtacgacc attcagcgta taacccttct gcataatgcc cagtacgtta cctggcgcaa
    18841 cgtcatcaga ttccaccatt gcgatagcct gatgcacatt cgggtccagc ggcacgttag
    18901 tttcggcgat cacttcaaca ccaaacttac gcacaacatc cagcatcgac ttcagcgtca
    18961 gctcaatgcc ttcaaccatc gcagacatat ccgggttagc tttatcagcc acttccagcg
    19021 cacgatccag gctatcaatc accggcagca attcgttgat gaatttctcc agcgcgaatt
    19081 tgtgggcttt ttcaatatcc agttcagtac gacgacgcag gttttccatt tcggctttta
    19141 cacgcaaaat gccgtcacgt tcacgggtct gggcttcagc cagctgagct tcgagattcg
    19201 caattttttc atcgcgcgga tccacctgct cagcagaagc ttctggctca actgcctcaa
    19261 tctcttcgtg ctgatccatg ataatttctt ccggggcttg cccctcaggc gttttctgtt
    19321 ctttactact catgaatttc tccgcgtttt tttcgcattc atctcgctaa cttcgcttat
    19381 tatggggatc agtttcaggg tttcaaggga agcactcaca ttgtcatcaa tcttcgcaac
    19441 aaggacctcg gaaaaatgaa taatcatttc aagtgtattg gcattgtggg acacccacgg
    19501 caccctactg cactgacaac acatgaaatg ctctaccgct ggctgtgcac aaaaggttac
    19561 gaggtcatcg ttgagcaaca aatcgctcac gaattgcaac tgaagaatgt gaaaactggc
    19621 acgctcgcgg agattgggca actggctgat ctcgcggtag tcgttggtgg cgacggtaat
    19681 atgctgggcg cggcgcgcac gctcgcccgt tacgatatta aagttattgg aatcaaccgt
    19741 ggcaacctgg gtttcctgac tgaccttgac cccgataacg cccagcaaca attagccgat
    19801 gtgctggaag gtcactacat cagcgagaaa cgttttttgc tggaagcgca agtctgtcag
    19861 caagattgcc agaaacgcat cagcaccgcg ataaatgaag tggtacttca tccaggcaaa
    19921 gtggcgcata tgatagagtt cgaagtgtat atcgacgaga tctttgcgtt ttctcagcga
    19981 tctgatgggc taattatttc gacgccaaca ggctccaccg cctattccct ctctgcgggc
    20041 ggtcctattc tgaccccctc tctggatgcg attaccctgg tgcccatgtt cccgcatacg
    20101 ttgtcagcac gaccactggt cataaacagc agcagcacga tccgtctgcg tttttcgcat
    20161 cgccgtaacg acctggaaat cagttgcgac agccagatag cactgccgat tcaggaaggt
    20221 gaagatgtcc tgattcgtcg ctgtgattac catctgaatc tgattcatcc gaaagattac
    20281 agttatttca atatattaag caccaagctc ggctggtcaa aaaaattatt ctaattttac
    20341 gccagcctct ttactgtata taaaaccagt ttatactgta cacaataaca gtaatggttt
    20401 ttcatacagg aaaacgacta tgttggcaca actgaccatc agcaactttg ctatcgttcg
    20461 tgagcttgag attgattttc atagcggcat gaccgtaata actggcgaga ccggcgcggg
    20521 taaatctatt gcaatagatg ccctcggtct ttgtctcggt ggtcgcgctg aagccgacat
    20581 ggtgcgtacc ggcgctgctc gcgctgacct gtgcgcccgt ttttctctga aagatacgcc
    20641 agcggccctg cgctggctgg aagaaaacca gcttgaagac gggcatgaat gtttgcttcg
    20701 tcgcgtgatc agcagcgatg gtcgctcccg tggtttcatc aacggtacag ctgttcctct
    20761 gtcacaactg cgcgaactgg gtcagttgct gattcagatc catggtcagc acgctcatca
    20821 gttactcacc aaacccgagc accaaaagtt cctgcttgat ggctatgcca atgaaacctc
    20881 tctactccag gaaatgaccg cacgttatca gttgtggcat caaagctgcc gtgacctcgc
    20941 gcatcatcaa cagttaagtc aggaacgcgc cgcccgtgcg gaactgctgc aataccaatt
    21001 aaaagaactt aacgaattta atccgcagcc cggagagttt gagcaaatcg acgaagagta
    21061 caaacgtctg gcgaacagcg gtcaattgct gaccaccagc cagaatgcat tggcattaat
    21121 ggccgacggt gaagacgcaa acctgcaaag tcagctttac acggctaaac aactggtgag
    21181 cgaattgatt ggcatggaca gcaaactgtc cggcgtactt gatatgctgg aagaagctac
    21241 catccagatc gctgaagcca gcgacgaact gcgccactac tgcgatcgtc tggatctcga
    21301 tcccaaccga ctgtttgaac ttgaacagcg catctcaaaa cagatttcgc tggcacgtaa
    21361 acatcacgtc agccctgagg cattaccaca gtattaccag tcgctactgg aagaacagca
    21421 gcaactggac gatcaggccg actcacaaga aacgctcgcg ctggcggtaa cgaaacatca
    21481 tcagcaggca ctggaaacgg cgcgcgcatt acaccagcaa cgccagcatt atgcagaaga
    21541 acttgcacag ctgatcaccg acagtatgca tgcgctctca atgccgcatg ggcagtttac
    21601 gattgatgtt aaatttgacg agcatcacct gggcgctgac ggtgccgatc gtattgagtt
    21661 tcgggtaacc accaacccag gtcagccaat gcagcctatt gccaaagtcg catccggtgg
    21721 tgaattgtcc cgcatcgcac tggcaatcca ggtcatcacg gcgcgtaaaa tggaaacccc
    21781 ggcactgatt tttgatgaag tggatgtagg gattagcggt ccaacagcgg cagttgtcgg
    21841 caaactgctg cgtcaactcg gcgaatcaac tcaggtgatg tgtgttaccc acctgccaca
    21901 agtcgcggga tgtggtcatc aacactattt tgtcagcaaa gaaaccgatg gtgcgatgac
    21961 agaaacgcat atgcaatctc tggataaaaa agcgcggtta caagagctgg cgcgcctgct
    22021 tggtggcagt gaagtcacac gtaacacact ggcgaatgcg aaagaactgc ttgcggcgta
    22081 aacttttttc ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac cggaaagcaa
    22141 aaggttttaa agtgatgaaa ggtctattat catcggcata ttacagatga gccacgtact
    22201 gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa aacgctgact gctgcagcag
    22261 cagtactatt gatgttgacc gcaggctgtt ccactctgga gcgagtggtt taccgtcctg
    22321 acatcaacca ggggaactat ctgaccgcta acgacgtatc caaaatacgt gttggcatga
    22381 cgcaacaaca agttgcgtac gcattgggta caccgctgat gtccgatcca tttggtacga
    22441 atacctggtt ctatgtcttc cgccagcaac caggtcatga aggtgtaacg cagcaaacgc
    22501 tgacgctaac ctttaacagt agcggtgtgt tgaccaatat tgataacaaa cctgcgctga
    22561 gtggtaacta ataataaagt tgctctcaaa gacgttaaaa aaggtgctca atgagcacct
    22621 tttttctgtc tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa gctctttcgg
    22681 atcggcaatg agagggcgat aaatctccac ccgatcgcca tcatgcacac tatcgcttag
    22741 ttttgccgga cggctgtaaa tgccgacttt gtttttagtt aaatcgatat cggtacgcaa
    22801 ctccagcaag ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct gcagcgtcac
    22861 gcgctgcagg tactgcttct caggtagcgc ataagccacc tcaacggcaa ttttacctgg
    22921 cactgtaaac ctctttcgca cgaaccgtaa aagcctggac catattagac gccagctctt
    22981 taaacacgcg accaaaagcg agttcaatca acttattggt aaactcaaag tcgagatgaa
    23041 actcgatacg acacgcctcc tggctcagcg gcgtaaactt ccatccacca atcaatttct
    23101 tgaacggccc atccaccaga ttcataagaa tactttggtt actggtcaac tggttgcggg
    23161 tagtaaacgt tttgctgatc ccagccttag agacatctac cgccgcagtc atctgcccag
    23221 gagtggactc cagaatccga cttccggtac aacccggcaa aaactgagga taagactgaa
    23281 cgtcattcac taactgatac atttgctccg cgctgtaggg taccagtgcg gtccggctaa
    23341 tctgaggcat aacaatttcc atcaacaaaa atccaacaaa taatatcatt tatcctgctg
    23401 taaaaaaaac gctatcccgg cgctgggtaa catcgggttc atgctaagat agagccttgt
    23461 cccccgcagg attgatatgg ggtgttttcg atttcagatt accgatgatt cacgacgctt
    23521 atgacgaaga aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa caagcgcgcc
    23581 cgtcacgaat actttatcga agaagagttc gaagcgggac ttgccctgca aggctgggag
    23641 gttaaatccc tgcgcgcagg aaaagccaat atcagcgaca gctacgtcct tctgcgtgac
    23701 ggagaggcat ttctgtttgg cgctaacatc acgccaatgg ccgtggcctc cacgcatgtg
    23761 gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc agcgcgaact ggactcattg
    23821 tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc tctccctgta ctggaaaaat
    23881 gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaga aacagcacga taaacgttca
    23941 gatatcaaag aacgcgaatg gcaggtggat aaagcgcgta tcatgaaaaa cgctcaccgt
    24001 taaacctgca ctccaattat tgaccagttc ctcaccgcgc ctccctctcc ggcggcgcga
    24061 atgaacatct tattggctat cacatccgac gaaaatgttg ccatcccatt gctaaatcga
    24121 ataaaaatca ggctacatgg tcgctaaatc tttaacgata acgccattga ggctggtcat
    24181 ggcgctcata aatctggtat acttaccttt acacattggg gctgattctg gattcgacgg
    24241 gatttgcgaa acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa gccgcaaaaa
    24301 atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat aacctgctta gagccctctc
    24361 tccctagcct ccgctcttag gacggggatc aagagaggtc aaacccaaaa gagatcgcgt
    24421 ggaggccctg cctggggttg aagcgttaaa acttaatcag gctagtttgt tagtggcgtg
    24481 tccgtccgca gctggcaagc gaatgtaaag actgactaag catgtagtac cgaggacgta
    24541 ggaatttcgg acgcgggttc aactcccgcc agctccacca aatgatgatc cggatacgtc
    24601 cggtgaagta cagaaagccc gcacggcaca agccctgcgg gcttttttgt gtctgtcgtt
    24661 gtccgagaac atccggctaa atccagagaa aattggtaca cgtttaggta cacgctatac
    24721 tgtggtccat taaacgtgta ccaattatgg aagggatcca gacatggcgc gcattacacg
    24781 ccccctcact aacaacgaaa tcctcaaagc gaaaccccgc gaaaaagact tcaccctgca
    24841 tgatggtgat ggcctgttct tactcgtcaa aacctccggt aaaaaactct gacgcttccg
    24901 ttatcaacga ccgggaagca gcagccgcac aaatttgagt ctcggctcat accctgccct
    24961 tacactcgca gcagctcgtc agatacgcga ccagcactta accacgctcg cgcaaggcat
    25021 ggatccacaa cagcaacagg agcaagcgtc agcacaacgc cagattgagt tagacagcat
    25081 tttctcaact gtggccgcta actggttcca gattaaaagc agaagtgtca cagaggatta
    25141 tgcaaaggat atttggcgtt ctttggataa agacgtgttc ccggcaatcg gtgcgatgcc
    25201 cgttcaggat ataaaggcca gaacgattgt tgaagcattg gagccaatca aagcacgtgg
    25261 cgcattagag acggttcgtc gcctagttca gcgtattaac gaaataatga tttatgcggt
    25321 taataccggt ctgatagatg ccaacccggc ttcgggtgta ggaatggctt ttgagaaacc
    25381 caaaaagcaa aatatgccca cgctacggcc agaagaatta cctaagctta tgcgttccct
    25441 ggtcatgtcg aatctgtctg ttccgactcg ctgtcttatt gaatggcagc ttctgaccct
    25501 agtgcgccct tctgaggctt ctggtgctcg gtgggtagag atcgatctca atgccaaact
    25561 ctggacgatt cccgccgaac gaatgaaggc taagcgtgaa cacattgttc ctttatcacc
    25621 acaagcatta gaaattctgg atgtgatgaa acctattagt gcacatcgag aacatgtttt
    25681 tcccagcaga aataatccaa aacaacctat gaatagccaa actgccaatg ctgcacttaa
    25741 gcgaataggt tatggtggga aattggttgc acatggttta cgttcgattg caagtacggc
    25801 aatgaatgaa gccggtttta atgctgatgt aattgaggct gctcttgctc attcagatag
    25861 aaatgaagta cgaaaagcct acaatcgctc aacatattta cacgcaagaa ttgaattgat
    25921 gaattggtgg ggacgacata ttcatcccca ccagtaaact cactctggag ttcctaaggc
    25981 agtaataaaa ccagcgtcaa ggaactccac attatcggat agactatcta tcaatgctct
    26041 tctccaatga ttaagcccat aacgtctggt atagtcatcc ctagttcttt ttagaaataa
    26101 tcttaatcct tcttggcctg cagaattata tgtttcttca agtgaagtat agagcccccc
    26161 taaaaaatca ttcgcttcca tctttaacct ttcatttagg ttaacaactc tattttgaaa
    26221 gcataatgaa tcatatacct ctcgagtcat accctcgatc atattgatat taaaatcaat
    26281 tactaaagca ccatctatca tttcagtatt ggcaacgaaa aatacaccat cgggctcatc
    26341 aaaataatac acatgaataa atccaactcc attttctgct acaccagctc tttttttatt
    26401 attgcactta tgacaggtcg gtatcaggtt tcttgaatat actgcgagac atttatatat
    26461 tgattcggga agataatgat ctaggtcggt tgatgattcg atcccacata atgggcattt
    26521 tttagaactt aataaaatcc ttgagcgaag ggttgaaagc cgtccattta tttgcacttc
    26581 tgaataagct gtttttatag cattttgggt ttcatcactt atttcctctg ct
//
LOCUS       DAZGBC010000059        26451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGBC010000059 DAZGBC010000000
VERSION     DAZGBC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.059"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..133)
                     /locus_tag="ACHK1J_002371"
     CDS             complement(<1..133)
                     /locus_tag="ACHK1J_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911622.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     "
     gene            complement(280..1563)
                     /gene="yeaH"
                     /locus_tag="ACHK1J_002372"
     CDS             complement(280..1563)
                     /gene="yeaH"
                     /locus_tag="ACHK1J_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO7911623.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1676..3610)
                     /gene="yeaG"
                     /locus_tag="ACHK1J_002373"
     CDS             complement(1676..3610)
                     /gene="yeaG"
                     /locus_tag="ACHK1J_002373"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO7911624.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4046..4792
                     /gene="mipA"
                     /locus_tag="ACHK1J_002374"
     CDS             4046..4792
                     /gene="mipA"
                     /locus_tag="ACHK1J_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO7911625.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4882..5736
                     /gene="yeaE"
                     /locus_tag="ACHK1J_002375"
     CDS             4882..5736
                     /gene="yeaE"
                     /locus_tag="ACHK1J_002375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO7911626.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASKCKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLTGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5787..6671)
                     /gene="yeaD"
                     /locus_tag="ACHK1J_002376"
     CDS             complement(5787..6671)
                     /gene="yeaD"
                     /locus_tag="ACHK1J_002376"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO7911627.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6755..7750)
                     /gene="gapA"
                     /locus_tag="ACHK1J_002377"
     CDS             complement(6755..7750)
                     /gene="gapA"
                     /locus_tag="ACHK1J_002377"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7911628.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8092..8505
                     /gene="msrB"
                     /locus_tag="ACHK1J_002378"
     CDS             8092..8505
                     /gene="msrB"
                     /locus_tag="ACHK1J_002378"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO7911629.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8547..8819
                     /gene="yeaC"
                     /locus_tag="ACHK1J_002379"
     CDS             8547..8819
                     /gene="yeaC"
                     /locus_tag="ACHK1J_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO7911630.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9189..10265
                     /gene="ydjL"
                     /locus_tag="ACHK1J_002380"
     CDS             9189..10265
                     /gene="ydjL"
                     /locus_tag="ACHK1J_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7911631.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPMCGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10292..11671
                     /gene="ydjK"
                     /locus_tag="ACHK1J_002381"
     CDS             10292..11671
                     /gene="ydjK"
                     /locus_tag="ACHK1J_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911632.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIHQTGKPLPPVVIADDGKTPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11688..12731
                     /gene="ydjJ"
                     /locus_tag="ACHK1J_002382"
     CDS             11688..12731
                     /gene="ydjJ"
                     /locus_tag="ACHK1J_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO7911633.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12752..13588
                     /gene="ydjI"
                     /locus_tag="ACHK1J_002383"
     CDS             12752..13588
                     /gene="ydjI"
                     /locus_tag="ACHK1J_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO7911634.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13593..14540
                     /gene="ydjH"
                     /locus_tag="ACHK1J_002384"
     CDS             13593..14540
                     /gene="ydjH"
                     /locus_tag="ACHK1J_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7911635.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14550..15530
                     /gene="ydjG"
                     /locus_tag="ACHK1J_002385"
     CDS             14550..15530
                     /gene="ydjG"
                     /locus_tag="ACHK1J_002385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO7911636.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGALDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            15667..16425
                     /gene="ydjF"
                     /locus_tag="ACHK1J_002386"
     CDS             15667..16425
                     /gene="ydjF"
                     /locus_tag="ACHK1J_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7911637.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16543..17901
                     /gene="ydjE"
                     /locus_tag="ACHK1J_002387"
     CDS             16543..17901
                     /gene="ydjE"
                     /locus_tag="ACHK1J_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911638.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVNVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSIFMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     ALGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17994..18635)
                     /gene="pncA"
                     /locus_tag="ACHK1J_002388"
     CDS             complement(17994..18635)
                     /gene="pncA"
                     /locus_tag="ACHK1J_002388"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO7911639.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(18646..19662)
                     /gene="ansA"
                     /locus_tag="ACHK1J_002389"
     CDS             complement(18646..19662)
                     /gene="ansA"
                     /locus_tag="ACHK1J_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO7911640.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(19794..20504)
                     /locus_tag="ACHK1J_002390"
     CDS             complement(19794..20504)
                     /locus_tag="ACHK1J_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFO7911641.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEDAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            complement(20620..22476)
                     /gene="sppA"
                     /locus_tag="ACHK1J_002391"
     CDS             complement(20620..22476)
                     /gene="sppA"
                     /locus_tag="ACHK1J_002391"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO7911642.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GPIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            22637..23188
                     /gene="ydjA"
                     /locus_tag="ACHK1J_002392"
     CDS             22637..23188
                     /gene="ydjA"
                     /locus_tag="ACHK1J_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO7911643.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQELSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            23305..24348
                     /gene="selD"
                     /locus_tag="ACHK1J_002393"
     CDS             23305..24348
                     /gene="selD"
                     /locus_tag="ACHK1J_002393"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO7911644.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            24353..26314
                     /gene="topB"
                     /locus_tag="ACHK1J_002394"
     CDS             24353..26314
                     /gene="topB"
                     /locus_tag="ACHK1J_002394"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO7911645.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFSPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
BASE COUNT         6665 a         6160 c         6799 g         6827 t
ORIGIN      
        1 taaaaaggag agcaatgtga gtgctggatt ttattgatgg cagagatata tagttttgac
       61 caggatcaat tttcgttaac gataaaaaat atttaaagaa taaccccatg gaaatacgtg
      121 ttgactgaat catttttccg aatgattgtt ttctggaact aaattatgta aaggttatgc
      181 tgtcatattt aaagcgattg taagctaatg tatgtaataa atgagataat ttacagtaag
      241 ctaaaaatca gccaggcgat aatgacccgg ctgatagttt tagtctttag ctgttgcatt
      301 ttgtttatga aacagttcac ggaacaccgg ataaatatca tcctggtcgc ggatgtgctg
      361 catcgcaaag ttgtcgaaag tagattgcag atgctcatat tctcgccaca gtgtctgatg
      421 cgcgcgacgg gtaatttcga tatagctgta ataacgaaca acgggtaata attttttcgc
      481 cagaatttca tggcaaagcg gagagtcatc ggcccagtta tcgccgtccg atgcttgtgc
      541 agcgtaaata ttccactgtg ccgggttata acgctctttc actacctcat ccatcagttt
      601 cagggcgctg gaaacaatgg tgccgccagt ttcctgcgag tagaaaaact catgttcatc
      661 gacttctttc gcctgggtat gatggcggat gtatacgact tccacgttct tatacgttct
      721 gctgaggaac agatacagca gaatataaaa acgcttagcc atatctttag tggattgatc
      781 cattgaaccg gaaacgtcca tcaggcaaaa catcactgcc tggctggagg gatcgggccg
      841 cttctcgtag ttcttgtaac gtaaatcgaa ggtgtcaata aaagggacgc gttcaatttt
      901 ggcacgtaat tctgcaattt ctttgcgcag acgttcctct tccagcagct gcgcaggttc
      961 actgttgctg atgatggcca aattctcttc cagtgcatga agttcccgcc gcttgcctgc
     1021 cgtcatggct gtgcgtcgcg ccagtgagtt ctgcaaggaa cgcacaacgc tgatattggc
     1081 cggaacgccg ttagcggtat aacccgcccg atgcgtttta tattcggtca gctggcgttg
     1141 ttggttttgt ttcagattcg gtaaggccaa atcttcaaag agcagatcaa gatactcatc
     1201 tttcgaaatc tgaaagacga attcatcctg accttcacca tcctggctgg cctggccctg
     1261 accactgccg gaacctccgc cgccaccctg gggacgttca attcggtcgt tctggacgaa
     1321 atggtcattg cccggatgta cgcggtggcg caggccgcca cgcccctgat gaaacatcgg
     1381 ttcgctaata tcttccgtgg gaatggatac ggattcgccg ctgtcgacgt cagtcaccga
     1441 acgcttatta atggcctcgg agatcgactg tttaatttgc gctttataac ggcgtaaaaa
     1501 acgctggcga ttcaccatgc ttttgttttt gccgttcaga cgccggtcaa taaaccaggt
     1561 catatgcccc ccgtactaca tttgccaact gtatgctacg cctgtgttgc catttctctg
     1621 gcccacggtt cgctgtcgtt gtaggcccgg tagcatgtgt accgggccag ggttgttaag
     1681 acgatttacg tacgcgcaaa taccattcgc acagtaaacg cacctgttta cgggtgtagc
     1741 ctttttccat catacggtcg acaaagtcgt cgtgtttctt ctgctcgtcg gttgacgttt
     1801 tggcgttaaa cgagataacc ggcaacagct cctcggtatt ggagaacatt ttcttctcga
     1861 taaccgtgcg cagtttttca tagctggtcc agttcggatt gcgtccgctg ttattggctc
     1921 tggcgcgcag tacgaagtta acaatctcgt tgcggaaatc ttttggatta ctgatccccg
     1981 ccggtttctc gattttctcc agctcggcgt taagagactc gcggtcaaac agctgcccgg
     2041 tatccggatc gcgatactcc tgatcctgaa tccagaaatc cgcgtaggta acataacggt
     2101 cgaaaatgtt ttgcccatat tcggaatagg attcaaggta ggccgtctgg atctctttgc
     2161 caataaactc ggcatatttc gggatcagat aacctttcag gaactccaga tagcgttctg
     2221 cctgctcttg tgggaactgc tcgcgctcga tctgctgttc caggacgtag aacagatgga
     2281 ccgggtttgc cgccacttct acatgatcga agttgaacac gcgggagagg atcttaaacg
     2341 caaaacgcgt cgacagaccg ttcatccctt catcgacacc ggcgtagtca cgatattcct
     2401 gatacgactt ggctttggga tcggtgtctt tcagactttc gccatcataa acccgcatct
     2461 ttgaataaat gctggagttt tctggctctt tcaggcgcga aagaatggaa aaacgtgaca
     2521 gtgtttcgag cgtgccaggg gcgcatgggg cgtgagtcaa ttcactgtga ttaagcaatt
     2581 tctcgtagat ttttatctct tcggaaatgc gcaagcaata cggcaccttc acgatgtaaa
     2641 cacgatcgag gaaggcttcg ttgtttttgt tattacggaa agtgacccat tcggactcgt
     2701 tcgagtgggc aagaataatc ccgttgaacg gcagggcgga gataccttcc gtcccgttgt
     2761 agttaccttc ctgagtggcg gttaacagcg gatgcagcac tttaatcggt gctttaaaca
     2821 tctcaacgaa ttccatgatc ccctggttgg cgcggcacag tgcgccggaa tagccatagg
     2881 cgtctgggtc attctgcgcg tagtgttcga gtttacgaat atcgactttc cctaccagcg
     2941 cggagatgtc ttggttgttc tcatcaccgg gctctgtttt ggcgatagca atttgttgca
     3001 gaatggacgg ccagaccttc actacccgga acttagtaat atcgccacca aattcatgca
     3061 gacgttttgc cgcccacggc gacatgatgg tgccgagata gcggcgagga atgccatact
     3121 ctttttccag aatctgcgca tcttcctgcg gattgaaaag acagaacgga tgatcgttga
     3181 ccgggctacg ctcaccgttg gcgctcaata cataaatcgg tacgagctgc attaatgatt
     3241 tcagtcgctc agcaagcgat gatttacccc cacccacagg ccccagcaga tacaggattt
     3301 gtttcttctc ttccagcccc tgagccgcgt gtttcagata agagacaatc tgttcaatcg
     3361 cgtcttccat gccgtaaaac tcttcaaatg ccggataacg tgcaatgacc cggttagaaa
     3421 agagtcgaga aagtctgggt tcctgggctg tatcgaccat gacaggctca ccgatagcca
     3481 tcaatagccg ctcagccgcg ttggcataag cactgcgatc ttgccgacaa gtggtaagaa
     3541 actcctgcag tgtgaactct tcgtccttgg cagcttcata tcgctggcga tagtgatcga
     3601 atatattcat ggtatgccgt cctttcgttt tttagcacag gttaagagcc gttcatatga
     3661 atagtagagg ctcccggaag aggtaaactg aacgacgtct cttgctaaaa cacagcaact
     3721 ctcatgccat tttatcgacg atgagaacaa cgtgcccggt gatacacctt caaaattaag
     3781 cgtagatggc aaatggaaaa cttgcctgca cggctttctt aatttcaatg gcatatcaac
     3841 aactcatcca taaattttgc ataattaatg taaagaccag ggttgccagt aacgttaaat
     3901 tcatttggct gtaagcgcgg tgtcatccgc gtcaggaaaa ttaaacagtt actttaaaaa
     3961 atgaaaacgt aaaaaggttg ggtttcgatg tattgacggg taaactttgt cgcccgctaa
     4021 acatttgttt aaggaatgat taattgtgac caaactcaaa cttctggcac ttggagtgct
     4081 tatcgcaacg tctgcaggcg tagcgcacgc tgaaggtaaa ttttccctgg gcgcaggcgt
     4141 aggtgtcgtt gaacacccat ataaagatta cgataccgat gtttacccag taccggtaat
     4201 caactatgaa ggcgataact tctggttccg tggcttaggt ggtggttact acctgtggaa
     4261 tgacgcaacg gataaacttt caattaccgc ttactggtcg ccgctttact tcaaagctaa
     4321 agacagtggc gatcaccaaa tgcgtcacct ggatgaccgt aagagcacca tgatggctgg
     4381 tctgtcttat gctcacttta cccagtacgg ttacctgcgt accaccctgg ctggcgatac
     4441 cctggataac agcaacggca tcgtctggga tatggcctgg ttgtatcgtt acaccaacgg
     4501 tggcctgacc gtgactccgg gtattggtgt gcagtggaac agcgaaaacc agaacgaata
     4561 ctattatggc gtatcgcgca aagagtccgc tcgcagcggt ctgcgtggct ataacccgaa
     4621 cgacagctgg agcccttacc tggagctgag cgccagctac aacttcctcg gcgactggag
     4681 tgtttacggt accgcgcgct acacccgtct gtctgatgaa gttactgaca gcccgatggt
     4741 ggataaatcc tggactggcc tgatttctac cgggatcacc tacaaattct gataatgcat
     4801 ctttccaggg cattttgtgc ataaaaacag ggcgtcacgc cctgttttgc attacggtgg
     4861 tgcaatacct gggaggaaac aatgcaacaa aaaatgattc aatttagtgg cgatgtctca
     4921 ctgccagcca tagggcaggg aacatggtat atgggcgaag atgccagtaa gtgtaaaaca
     4981 gaagttgctg cactacgcgc gggcattgag ctcggtttaa ccctcattga taccgccgaa
     5041 atgtatgccg atggtggtgc cgaaaaggtg gttggggaag cattaaccgg tctgcgtgat
     5101 aacgtctttc tcgtctctaa agtctatccg tggaatgctg gcgggcaaaa agcgataaat
     5161 gcatgcgaag ccagtttacg ccgtctcaat actgattatc tcgatctcta cttattacac
     5221 tggtctggca gtttcgcttt tgaagagact gtcgcagcga tggaaaaatt gatcgcccaa
     5281 ggaaaaatcc gccgctgggg cgtttcgaac cttgattatg ctgatatgca ggaactctgg
     5341 cagctgacgg ggggaaatca gtgtgccact aatcaggtgc tttaccatct cggttcacga
     5401 gggattgagt acgatctact cccctggtgc cagcaacagc agatgccggt gatggcttac
     5461 agtccgttag cccaggccgg gcggttgcgc aatggactgt taaaaaacgc ggtagtcaac
     5521 gaaattgcac atgctcacaa tatcagcgcg gcacaagtat tgttggcgtg ggtaatcagc
     5581 catcagggtg tgatggcgat tccaaaagcg gccacgattg cccatgtcca acagaatgcg
     5641 gctgtgcttg aggtcgaact ttcgtcagcg gaattagcca tgctggacaa agcatatccg
     5701 gcaccaaaag gaaaaactgc gctggatatg gtgtgatgtt gatacgtgcc ggatgcatcg
     5761 cacccggcac acaacattaa cgtaaattaa cgtttcgcaa cgcgaatgga ttgcgccaga
     5821 tgtgcaggtt tttctttggt cactttttgc gtttctgaag cgtaagccgt ttctacacaa
     5881 acaaaggtct tgtagccatc gtccggcata tcgcccatgc taactgaaag cgccggtccc
     5941 gggttccagc ctacaacgtt cagatgatgc tggtggccta cggcgataat acgattcagc
     6001 gcttcatcat taatcacgct gcaatcttgc ggattcagat atacgcgatc ggtacggtca
     6061 gggaaggtct gaataccgtc ggtcagtaca tcttctttcg cgtcatttac tttatcaatg
     6121 aagcgatcgc ccagcccact gacgcttacc ttagcgatat cacccacgtt aaagtaggta
     6181 tgcagggcag aggtggtttc aaattcgccg tgtgcttcaa gatcgatttc acaggtttta
     6241 cccacgcgga aatgcgctaa cagcgtaaag tcgtgcggcc agaatttttt cgtctcttcg
     6301 ctttgtgtca attcaaaagt cagcgctacg ccatcagcat cttcacgatg tgatttcagc
     6361 gtccacggca ggttgcgggc aaaaccgtgc gcaggcaggc cttgttgtgc cgccggacca
     6421 aaccacggcc agcaaaccgg tacgccaccg cgaatagcga cgccattttt gaacggtgtg
     6481 ttgttgctca accacagaac ttcttcttca cccgcaggtt tccacgagag aaggtgtgcg
     6541 ccctgtaatg caaaagaggc ttttacctgg ggatgatcga ccacaatgag gtccagttca
     6601 tccagtttac gacgggagag gacaggggag atttgttcga tgaccggaag ggcaaaaatt
     6661 ttcttaatca tgacgcagtc ctttaacttc attttatcag gtaaaaaaaa gagcgaccga
     6721 agtcgctctt tttagatcac agtgtcatct caacttattt agagatgtga gcgatcaggt
     6781 ccagaacttt gttggagtaa ccggtttcgt tgtcgtacca ggataccagt ttcacgaagt
     6841 tgtcgttcag agcgatacca gctttagcat cgaacacgga agtgcaaact tcgccgttga
     6901 aatcggtaga tactacgtca tcttcggtgt agcccagaac gcctttcatt tcgccttcag
     6961 cagcagcttt aacggcagct ttgatctgct cgtaagttgc agctttttcc agacgaacgg
     7021 tcaggtcaac cacggatacg ttcggggtcg gaacgcggaa cgccatacca gtcagtttgc
     7081 cattcagttc tggcagtact ttacctacag ctttagcagc accggtagag gacgggatga
     7141 tgttctggga agcgccgcgg ccgccgcgcc agtctttgtg agacgggcca tcaacggttt
     7201 tctgagtagc ggtagtagcg tgaacggtgg tcatcagacc ttcgatgatg ccgaagttat
     7261 cgttgataac tttagccagc ggagccaggc agttggtggt gcaggaagcg ttggaaacga
     7321 tgtcctggcc agcatatttg tcgaagttag cgcctttaac gaacatcgga gtgttgtctt
     7381 tagacggacc agtcataacc actttcttcg caccagcggt gatgtgttta cgagcagttt
     7441 cgtcagtcag gaacagacca gttgcttcag cgacaacgtc aacaccaact tcgtcccatt
     7501 tcaggttagc cggatcacgt tcagcggtaa cacggatttt tttaccgtta acgatcagat
     7561 gaccgtcttt cacttcaacg gtaccgtcga aacggccgtg agtggagtca tatttcagca
     7621 tgtatgccat gtaatcagcg tctaacaggt cgttgattgc aacgatctcg atgtcagaac
     7681 gtttctgagc agcacggaaa acaatgcgac cgatacggcc aaaaccgttg atacctactt
     7741 tgatagtcat atattccacc agctatttgt tagtgaataa aaggttgcct gtaaaattac
     7801 aaaaacctta cgcagcgtca agcggaatcg tgtcaatcat tgcgacaaat caatcctgtg
     7861 cctaagcatt acgcgactga ctcgcctcac tcttcctttg ggcttgagac cacatggggt
     7921 cggcgccccg aattttaaag ggcaattacg ataaaaatgt gattttcatc acgatttagg
     7981 tgcagccatt tcgtcgagat caagtttagg acaaaatttt gccagttggt gttaatgttt
     8041 tgttagaatc agtcacgtaa tgtgagcacg attaaagtga gatgtgagca aatggctaat
     8101 aaaccttcgg cagaagaact gaaaaaaaat ttgtccgaga tgcagtttta cgtgacgcag
     8161 aatcatggga cagagccgcc atttacgggt cgtttactgc ataacaagcg tgacggcgta
     8221 tatcactgtt tgatctgcga tgccccgctg tttcattccc aaaccaagta tgattccggc
     8281 tgtggctggc ccagtttcta cgaaccggtg agtgaagaat ccattcgtta tatcaaagac
     8341 ttgtcacatg gaatgcagcg catagaaatt cgttgcggta actgtgatgc ccatctgggg
     8401 catgtcttcc ccgacgggcc gcagccaacg ggcgaacgtt attgtgttaa ctctgcttct
     8461 ttacgcttca ctgatggcga aaacggcgaa gaaatcaatg gttgaaaaaa cgattcagca
     8521 acttattcca caggagcgga aaaattatga atcttgatga cattatcaac agcatgacgc
     8581 ctgaggtata ccagcgtttg tcgaccgccg ttgaactggg gaaatggcct gatggcgttg
     8641 cgttgaccga ggaacaaaaa gagaactgcc tgcaactggt gatgctgtgg caagcccgca
     8701 ataataccga agcacagcat atgacaattg acacaaatgg tcagatggtc atgaagagca
     8761 aacaacagtt aaaagaagat tttggtatct cagcaaagcc tattgccatg tttaagtaag
     8821 tggtttgtcc cctttttgcc gcggttataa aggggatagc caacaataac cccacgactc
     8881 atctcaatta tctcgtcacg accaggcaaa gccccagccc ggtctcttgc cgtcgcgttc
     8941 acgcctgaat gacttctcct gaacttaagt ctcagaccta tttggccggt aatccctctc
     9001 aaatttgctc cttttgcgtt cagccttcca tcaacctgta acagaaccaa ccattttctg
     9061 cgtaattgtg atctgagttg taaaacgcag atcattatct gtgttttaca gatttaatgt
     9121 tgacctcaaa aatcatgtgg gtaagtatga tgaccatcta agcaaaacaa aacacagagg
     9181 atgacgcaat gaaagcactg gctcggtttg gcaaagcctt tggcggctac aagatgattg
     9241 atgtcccaca acccatgtgt ggcccggaag atgtagtgat tgaaattaaa gccgcggcaa
     9301 tctgcggcgc agacatgaag cactacaatg tcgatagcgg ttctgatgag tttaactcta
     9361 tccgcggcca tgagttcgca ggttgtattg cgcaggttgg tgaaaaagtc aaagactgga
     9421 aagtggggca acgcgtcgta tcggataaca gcggtcacgt ttgcggcgtt tgtccggcct
     9481 gtgaacaagg tgattttctg tgttgtacag aaaaagtaaa ccttggtctg gataataata
     9541 cctggggcgg tggtttttcc aaatattgtc tggttcctgg tgaaattctc aaaattcatc
     9601 gtcatgcgtt gtgggaaatc cctgatggtg ttgattatga ggacgcagcc gtacttgacc
     9661 ctatctgtaa tgcctacaaa tccatcgcgc agcaatcgaa attccttcct ggtcaggatg
     9721 tggtcgtcat cggcactggc ccactcgggc tgttctccgt acaaatggcg cgaattatgg
     9781 gggcggtaaa tatcgtcgtc gttggtctgc aagaagatgt ggcggtccgc ttcccggttg
     9841 caaaagaact gggtgcgacg gcagtagtaa atggttctac cgaagatgtg gtggcgcgct
     9901 gccagcaaat ttgtggaaaa gacaatctgg gactggtgat tgaatgctcc ggtgccaata
     9961 tcgcactgaa acaagccatc gaaatgctcc gcccgaacgg ggaagtggta cgcgttggaa
    10021 tgggcttcaa acctcttgat ttctcgatta atgacattac cgcctggaac aaaagcatca
    10081 ttgggcatat ggcctatgac tccacctcat ggcgtaacgc tatcaggcta ttagccagcg
    10141 gcgctatcaa agtcaaaccg atgatcacgc atcgtatcgg cctgtcgcaa tggcgcgaag
    10201 ggtttgatgc gatggtcgat aaaaccgcaa tcaaagtgat catgacttac gactttgatg
    10261 aataacaact ttcctgaagg aacgttatcg catggaacag ataacaaaac cgcattgtgg
    10321 tgcacggctg gatcgcttac cggattgccg ctggcattcg tcaatgtttg ccatcgtcgc
    10381 gtttggcttg cttgtctgct ggagtaatgc cgttggtggc ttgatcctcg cgcagctgaa
    10441 agcgttgggc tggacagata attccaccac agccacattc tcagcaatca cgaccgccgg
    10501 aatgtttctc ggtgcgctgg tgggcggcat cattggcgac aaaaccggtc gcagaaatgc
    10561 gttcatcctc tatgaggcca ttcatattgc ctcgatggtt gtcggtgctt tctcaccgaa
    10621 tatggatttc cttattgcct gccgttttgt gatgggagtt gggctgggag ctttactggt
    10681 tacgctgttt gctggtttca ccgaatatat gcccggtaga aatcgtggaa cgtggtcaag
    10741 tcgggtttcc tttattggca actggtcata tccgctctgt tcattgatag cgatgggact
    10801 cacgccgctg attagtgcag agtggaactg gcgggtacaa ctgcttatcc ctgcaatatt
    10861 gtcgcttatc gctacggcgc tggcctggcg ctactttcct gaatccccgc gctggctgga
    10921 atcgcgcgga cggtatcagg aagccgagaa agtcatgcgg agtatagaag aaggcgtcat
    10981 acaccagacg ggcaaacctt tgccgcccgt ggttattgct gatgacggta aaacgccaca
    11041 agcggtgccg tattcagcct tactgacagg agtattactg aaacgcgtga tattaggctc
    11101 ttgtgtgctg attgccatga acgttgtgca gtacacacta attaactggt tgccaacgat
    11161 attcatgacc caggggatta atttaaaaga ctcgattgtt ttaaatacca tgagtatgtt
    11221 tggcgcgcca tttggcattt ttattgccat gctggtgatg gataaaattc cgcgtaaaac
    11281 aatgggtgtg gggctattaa tcctgattgc ggtgctcgga tatatctatt cactgcaaac
    11341 cagtatgttg ctcataacgt tgattggttt cttcctgatt acttttgtct atatgtacgt
    11401 ttgctatgcc tcggcagtgt atgtccctga gatctggccg acagaggcca aactccgtgg
    11461 ctccggtctg gcgaatgcgg tagggcgaat cagtggtatt gccgcacctt atgccgttgc
    11521 agtgctgctc agtagttatg gcgttacggg agtctttatt cttctggggg cggtttcaat
    11581 tattgtcgca attgctatcg ccaccattgg aatcgaaacc aaaggtgtct ccgttgaaag
    11641 tttaagtatt gatgcagtag ccaataaata atacgggtgg attcaaaatg aaaaattcaa
    11701 aagcaatatt gcaggtgccg ggcacaatga aaattatttc agcagaaata ccagtgccta
    11761 aagaagatga agttttgatt aaagtagaat atgtcggtat ttgtggttca gatgtacatg
    11821 gttttgaatc aggtccgttt attccgccta aagacccaaa tcaagaaatt ggcctgggtc
    11881 atgaatgcgc cgggacggtt gtggctgtgg gaagccgcgt gcgcaaattt aaaccggggg
    11941 atcgggtaaa tatcgaacct ggcgttcctt gcggtcactg tcgttactgt ctggaaggca
    12001 aatataacat ctgcccggac gttgatttta tggcgacaca acccaactac cgcggcgcat
    12061 taacacacta tctgtgtcat ccggagagct ttacttacaa actgcccgac aatatggaca
    12121 cgatggaagg ggcgctggtg gagcctgccg cagtcgggat gcatgccgcg atgctggcag
    12181 atgttaaacc gggtaagaag ataattattc tgggagcagg ttgtattggt ttgatgacgt
    12241 tgcaagcgtg caaatgcctg ggagcaactg aaattgccgt cgttgatgtg ctggaaaaac
    12301 gtctggcaat ggcggaacag cttggtgcga cagtggttat taacggcgca aaagaagaca
    12361 ctattgcacg ctgtcagcaa tttaccgaag acatgggcgc agatattgtt ttcgaaacag
    12421 cgggttctgc ggtcaccgtt aaacaggcac cttatctggt aatgcgcggc ggtaaaatta
    12481 tgattgttgg tactgtaccc ggcgattcgg caatcaattt cctcaaaata aatcgcgaag
    12541 ttactatcca gacggtattc cgctacgcca atcgttatcc ggtcacgatt gaagctattt
    12601 cttcagggcg attcgatgtg aaatcgatgg tgacgcatat ttacgattat cgggatgtac
    12661 aacaggcatt tgaagagtca gttaacaata aacgcgacat tattaaaggc gttattaaga
    12721 ttagcgatta attaatacgg gagaacagaa catgctcgca gatatcagat attgggaaaa
    12781 cgatgccaca aataaacgtt atgctattgc ccattttaat gtatggaatg cagaaatgtt
    12841 gatgggcgtt atcgacgcag ccgaagaagc gaaatcaccg gttattattt cttttggtac
    12901 aggttttgtc ggcaacacct catttgaaga tttctctcac atgatggtat caatggcacg
    12961 aaaagcaacg gtgccggtaa taactcattg ggatcatggt cggagtatgg agattattca
    13021 taacgcctgg actcatggta tgaattcatt aatgcgtgat gcttccgcat ttgatttcga
    13081 agaaaatatt cgcttaacca aagaggctgt cgatttcttc catccgctgg gtattccggt
    13141 agaggcggaa ttagggcatg tcggtaatga aaccgtttat gaggaagcgc tggcgggcta
    13201 tcactatacc gatcctgacc aggcggctga atttgttgaa cgtacgggct gtgactcatt
    13261 ggctgtcgcc atcggcaacc agcatggggt ttatacgtca gagccacaat tgaactttga
    13321 ggtcgtcaaa cgcgtacgcg atgccgtgtc tgttccgctg gttttgcatg gcgcatcggg
    13381 gatcagtgat gccgacatta aaactgcaat ttcgttgggt atcgcgaaaa tcaacatcca
    13441 tacggagctc tgtcaggccg caatggtcgc agtgaaagaa aaccaggatc agcctttcct
    13501 gcatttagaa cgtgaagtac gtaaagctgt aaaagaacgg gcattggaaa aaattaaact
    13561 gttcggttct gatggcaaag cggaataatg atatggataa tctcgacgtt atttgtatag
    13621 gtgccgctat tgttgatatt ccattgcaac cggtcagtaa aaatatcttt gatgtggatt
    13681 cttatcctct tgaaagaatc gcaatgacca ccgggggtga tgcaataaat gaagcaacaa
    13741 ttatttctcg tctgggccat cgtacagcat taatgagccg tattggtaaa gatgccgcag
    13801 ggcagtttat tctcgaccat tgccgcaaag agaatattga tattcaaagc ctgaagcagg
    13861 acgtcaacat agatacctct attaatgttg gactggtgac ggaggatggc gagcggacgt
    13921 ttgtcacaaa ccgtaacggc agtctgtgga aattgaatat tgacgatgtt gattttgcgc
    13981 ggttttccca ggcgaaatta ttatcactgg ccagtatttt caacagtcca ctattggatg
    14041 gtaaagcgct aacagaaatt tttacgcaag ctaaagcccg gcagatgatt atctgtgccg
    14101 atatgatcaa accgcggttg aatgaaacgc tggatgatat ttgcgaagca ttgagttatg
    14161 tcgattatct gtttcctaat tatgccgagg caaaattact caccgggaaa gagacactgg
    14221 atgaaattgc tgactgcttt cttgcgtgcg gcgtaaaaac ggtggtgatt aaaacgggta
    14281 aagacggctg ctttatcaag cgtggtgaca tgacgatgaa ggtgccggcg gtcgcaggaa
    14341 taaccgccat cgacaccatt ggcgcgggcg ataactttgc ttcaggtttt attgcggcac
    14401 tgttagaagg aaaaaatctg cgtgaatgcg cacgctttgc caatgcaacg gcggctatct
    14461 cggttctaag cgtcggtgcc accaccggcg taaaaaacag aaagctggtc gaacaattgc
    14521 tggaagaata cgaaggataa tgaaggcaaa tgaaaaagat acctttaggc acaacggata
    14581 ttacgctttc gcgaatgggg ttggggacat gggccattgg cggcggtcct gcatggaatg
    14641 gcgctctcga tcggcaaata tgtattgata cgattcttga agcccatcgt tgcggcatta
    14701 acctgattga tactgcacca ggatataact ttggcaatag tgaagttatc gtcggtcagg
    14761 cgttaaaaaa attgccccgt gaacaggttg tagtagaaac caaatgcggc attgtctggg
    14821 aacgaaaagg aagtttattc aacaaagttg gcgatcggca gttgtataaa aacctttccc
    14881 cggaatctat ccgcgaagag gtagaagcca gcttgcaacg tctgggtatt gattacatcg
    14941 atatctacat gacgcactgg cagtcggtgc cgccattttt tacgccgatc gctgaaactg
    15001 tcgcagtgct taatgagtta aaggctgaag ggaaaattcg cgctataggc gctgctaacg
    15061 tcgatgctga ccatatccgc gagtatctgc aatatggtga actggatatt attcaggcga
    15121 aatacagtat cctcgaccgg gcaatggaaa acgaactgct gccgctatgt cgtgataatg
    15181 gcattgtggt tcaggtttat tccccgctag agcagggatt gttgaccggc accatcactc
    15241 gtgattacgt tccgggcggc gctcgggcaa ataaagtctg gttccagcgt gaaaacatgc
    15301 tgaaagtgat tgatatgctt gaacagtggc agccactttg tgctcgttat cagtgcacaa
    15361 ttcccactct ggcactggcg tggatattaa aacagagtga tttaatctcc attcttagtg
    15421 gggctactgc accggaacag gtacgcgaaa atgtcgcggc actgaatatc aacttatcgg
    15481 atgcagacgc aacattgatg agggaaatgg cagaggccct ggagcgttaa atactcaaca
    15541 cgattcacct ctgtttctgt gaaaactgtg atgaaaatct gtgattcaaa caggttattt
    15601 tgaaagtaaa catcggttat gcgataatcg cgctaatgtg atgtaaaaag atacaggggt
    15661 gtaatcgtgg cggcaaaaga caggattcag gcaattaagc aaatggttgc caacgataaa
    15721 aaggtgacag tctcaaattt gagtgggatt tttcaggtaa ccgaagaaac cattcgccgc
    15781 gatcttgaga agctggaaga tgaaggcttt ttgaccagaa cctatggtgg tgctgtttta
    15841 aatacagcga tgttgacgga gaatatccat ttttataagc gcgcttcatc gttttatgaa
    15901 gagaagcagc ttattgcacg caaggcacta ccctttatcg acaataaaac caccatggca
    15961 gccgattcca gtagtaccgt aatggaattg ctcaaattgt tacaggaccg tagtgacctg
    16021 acgttgctaa ccaactcggc ggaagcgatt catgttctgg ctcagtcaga aattaaagtc
    16081 gtttcaacgg gtggggaact aaacaaaaat acactttcac tgcaaggaag aattactaaa
    16141 gagatcatca gccgctatca tgtcgatatc atggtaatga gttgtaaagg tcttgatatt
    16201 aacagcggcg cgctggactc taatgaagcg gaagctgaaa tcaaaaagac aatgatccgt
    16261 caggcgacag aagttgcgtt attggttgat cactctaagt ttgatcgcaa agcttttgtc
    16321 cagttagctg actttagtca tattaattac ataataactg ataaatcacc gggtgcagag
    16381 tggatagcat tttgcaaaga caataatatc cagctggtgt ggtaatttta gcacacgttg
    16441 tcaatattat ttgttcctgt gcctggaaat aaaatgatga taaagatgcg gcaagtgaat
    16501 ttgccgcaat tgctaccttt tttagcaaat ccgaggcacc caatggaaca atatgatcaa
    16561 attggcgcaa gactggaccg cttgcctttg gcccggtttc attatcgtat atttggtata
    16621 ataagcttta gtctgttatt aacggggttt ttgagttact caggtaatgt cgtcttagca
    16681 aagctggtaa gcaatggatg gtcaaataat ttcctcaatg ccgcctttac ctcggcatta
    16741 atgtttggtt atttcatcgg ctctcttacc ggtgggttca tcggtgatta ctttgggcgg
    16801 cgcagggcgt ttcgcataaa tcttctcatc gtcggtattg ctgcaacagg ggccgctttt
    16861 gtccctgata tgtactggct catcttcttt cgcttcctga tgggaacagg aatgggggcg
    16921 ctgattatgg ttggctatgc ctcatttacg gagtttatcc ccgcgacggt gcgtggaaaa
    16981 tggtccgcgc ggctctcatt tgttggtaac tggtcgccca tgctgtctgc ggcgataggt
    17041 gtggtggtta tcgctttttt tagttggcgg ataatgtttc tgttgggtgg tattggcata
    17101 ctgttagcct ggtttctctc aggtaaatac tttatcgagt cgccacgatg gctggcaggg
    17161 aaagggcaaa tcgccggtgc agaaagccaa cttcgtgaag tagagcagca aattgaaaga
    17221 gagaagagta ttcgtttacc cccgcttact tcgtatcaga gcaacagcaa ggttaacgta
    17281 atcaagggta ctttctggct cctgtttaaa ggggaaatgt tacgacgtac attagttgcg
    17341 ataactgttt taattgcaat gaacatttcg ctttatacca ttaccgtatg gataccgacc
    17401 atatttgtta actccggcat tgatgtcgat aaatcaatat tcatgaccgc tgttattatg
    17461 attggcgctc cggtaggaat atttattgcg gcattaatta ttgatcattt tcctcgtcga
    17521 ttatttggct ccgccttact tattattatt gctgtgttag gctatatcta ttcaattcag
    17581 actacagagt gggcgatttt aatctatggt ctggtgatga tcttcttttt atacatgtat
    17641 gtttgctttg cgtcggcggt ttatatcccg gaactttggc caacgcattt acgcctgcgt
    17701 ggttcgggtt tcgttaatgc cctcggccgg atcgtcgcag tcttcacgcc ctatggcgtt
    17761 gcggcattat taacacatta tgggtcaatc acggtgttta ttgtgcttgg tgtgatgtta
    17821 gtgctctgcg cgctggttct ctccattttt ggcatcgaaa cgcggaaggt gtcgctggaa
    17881 gagatttctg aggtgaacta aatctttcgt tgatggcgtc cattaccaga tgtaccactg
    17941 acgtgtttta tctggcctac aatgccagta cagaactgta ggccagcgta aaattacccc
    18001 tgcgtctctt cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata
    18061 aacgcgtgtg cactgtcctg tggctgaata ttcacgccac gacaaccatc ggtaatcacg
    18121 ttcaccttat aacctaactg taacgcatcc agcacggtaa acttcacgca atagtcagta
    18181 gccaggccca taacgatcaa ttcatcgatt tcatgatcgc gtaaccagtc atcgagcgtg
    18241 gttttctgcc gacggccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg
    18301 cctttatgga acaccgctgc gatcgctttt tggttcagta acggatgtaa ttgtgcgcct
    18361 tcactgttct gcacacagtg atctggccag aaggtttgtg gcaaaccgtc gagttggcct
    18421 ggcgtataag gctctacacc gtgctgactg gcaaaactgc cgtgattcgc cgggtgccag
    18481 tcctgactgg cgataaccgc ttcaccgcgc gactggcacc agtcaatcag gcggttagcg
    18541 acatccaccg tactgtcacc ttccggcacg gcgagcgcgc caccagcaca gaaatcattt
    18601 tgtaaatcga ccagtaacag ggcgcgaggg ggcattgcag tctccttaat catccggcgt
    18661 cagttcgccg cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg
    18721 gctaagcagg taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat
    18781 aacgccagca tgggcgaggg cgttaccggt ggcataacca cccatattca ctttaccgga
    18841 catacattgt gtcaggttga ccactacaat accgcgatcg ctggcttctt gtaattcctg
    18901 caggaaggct ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt
    18961 caccggttgg cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac
    19021 tacgccaatt ggttgtgggg tgattggatg aacgattaat tccccttcac cgtgcggggc
    19081 gggtggcgta ttcaaacgac gaatatggat acctgcttcc agtaacggag gaaggtttgg
    19141 agaggcaaac gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacag
    19201 tcggttatta aaaaagagtg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc
    19261 attcagcaga ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt
    19321 cacaatgacc ggtttaccga gattttcgag catgaacgac agcgcagagg cggtatacgc
    19381 catcgtgtcg gtgccgtgca gaatgacaaa accatcatag tcgtcatagt gcgctttaat
    19441 atcttcagca atatgctgcc agtcttccgg cgtcatatct gaagaatcca tcagcggcgt
    19501 atattcatga atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag
    19561 ttggcgttgt agatgacctg acaccggtat ataaccctgc tcggaacgct gcatcccgat
    19621 ggtcccgccc gtgtaggcaa cgtaaattga tttcttttgc atgatattga tatgaatgat
    19681 atcgaaagag caaaagtata tacgtgaggg gcagcgagcg cacctggata ctctgtggat
    19741 atgagaaaag tccggcagga cggagggatt agtgtcccgc cggagaaaag tggttaatcc
    19801 tgcaacgcat aggttacggt cgtgactacg cgtaccgttt tattaatcgc cgtaggatca
    19861 tcttcatcac tgccactggc aaagatctgg aatacgcctt gtgaggcatc gctaattgac
    19921 cccacctggc tacctgagtc cgttgcaaat tgtagtgcgg cgttgcgtgc atctttggtg
    19981 gcggcagcaa tcatctcagc gcgcagtgcg ccaataccat taaagcgata ggcgactttg
    20041 ctctcagcga tagcgccatc cgtagcgact tgcagttcgt ttaatttcgc gacgactttt
    20101 tcgacagcag caacattatg ggtttttatc gtcactgacc cgtctgcgtt atagcgtggc
    20161 attgtgggat catctttcag atagaaggaa tctgttcgct tgcttgtcca ttggcccagg
    20221 attatctctt tctcgttaat cccctgagca ttgagttcgg caacaatttt tttctgcgcg
    20281 tcttccagtt tcgggaacag ttcgccggga acattaccct taaagttaat cgcaattgtt
    20341 aattctgccg tatctgcgcg aacttctttt tcagaaagcc cgcgcacatt cacataacgg
    20401 ttgttggttt ttaaatgttt aacaccatcg ccaacaaaat aaccacaggc gctaatcccc
    20461 agtccgagga cgatagcggc aattaaagag ggggccttgt tcactgatga ctccatatca
    20521 gatgctaaat gagcaaggga atgtagtgga gagagggagt gaaagacagt ccgattcagg
    20581 agtgtggccg acagcatatc gaccacattt ttgcaggatt taacgcacgt tggcgcaggt
    20641 caggcaaaac gcataacggt tttgtgggtc gttaaacgcg gccagcttgt cactttcact
    20701 tttaacggta gaggctaccg aggcaagcgg tgcaggtaac atggcctgga acgcatctgg
    20761 caacattgcc cggacagaac cagacatgtt gtccatcact ttgtcgaaga aggtcggttc
    20821 atcaacgtag tattccagat gccactgttt cacttttgcc agctctgctg ctttggcgac
    20881 cgcatcatcg aaatccccga gactatcgac cagcccgtta gcttttgcgt cctgaccggt
    20941 ccagacgtgg ccctgggcga ttttatcaat ctgctccggc gtcgaatgac gcgcatcagc
    21001 aaccagcgta ataaagcgtt tatagccatt ctcaatgctt aactgcatca tctgctgcgc
    21061 ttccggcggc agtgccctgg tgatagaaac atctgccagc ggtgaagttg agacaccatc
    21121 agtatgaaca ccaatcgaat ccagactatt ttctacggtg gtgatcacgc cgaagatacc
    21181 gatagaaccg gtcagggtgc tggggttagc cacaatgtaa ttagctggcg tagaaatcca
    21241 gtaaccacca gatgccgcca tgccgcccat cgatacaacc acaggcttac ccgctgcccg
    21301 ggctgctgcc agttcagcgc gaatcacttc agacgcggta acgctgccgc ctgggctatt
    21361 aacacgcagg acaatcgctt tcactttcgg gtcaaggcga gcgtcgcgga tttgtgccgc
    21421 agtggtatca ccgccaacat tcccctgagt ttcctcgcca tccataattg ggccattagc
    21481 gaagacgaca ccgatgctgt caccggtatc tgccggcgtt ttcaatgcgt aatcgtaata
    21541 actgatggcg cgataatttt tatcagtctt actccagccg aactctttgg tcagagcttt
    21601 ttcgatttcc gcactcgatg ccagtgcatc gaccagcttg ttttccagtg catatttcgc
    21661 ggtatcgcca ccggttttgg ttaaaccctc aagcaaccct tgcgcgccag ggaatacctg
    21721 ctgagcaggg atctgccggt tagcggcaac agtattcaga tagttttgcc acagctcacc
    21781 aatccagcgg ctgtcagctt cgcgggctgc cggtgacatg tcatcacgaa taaacggttc
    21841 aacggcagat ttatacgtac ccacgcggaa cacatgggtg gaaactttca gcttatccag
    21901 caacgatttg tagtacagac cgttggtagc aaaaccgtgc aaatccacca cgccttgcgg
    21961 tgacagccaa attttattgg cgaaactggc gagataatat tgcccctggc tgtagttctc
    22021 gccaacggca taaaccggtt tcccgctgtc acgaaactct ttcagggctt tgccgatgta
    22081 ctgcatagac ggctggtcgc cgcctgcgaa gtttttcaga tccatcacga taccggtgat
    22141 attgcggtcg tccttcgcct ggcgaatggt gttgacgata tcaaacagtg agttttcctg
    22201 cagacgatcg gaactggcac caagtagttg gcggcttaat ttactaaacc gctgagaact
    22261 gtcgggttta tcgacgatta caccagaaat gtccagcagc agtgcaccac gactggccgt
    22321 ttctttcgaa tcaccaccac tgacctgcat ccagatcccc acaccaacca gcacgagaaa
    22381 aataaagaac aggttaagta ccatttcacg gacgaaattc agcagacgcc acgtccattt
    22441 aaaaaatccg gcaataaatc gccaaagggt tcgcatgtat tctcccaact taaggtcaca
    22501 cctgtcacag gcgcaataga tgtggttatc gtaatgaccc aaccgacact tgtcagcagg
    22561 aattgcggac tgtgctgtaa caaaatccac tctcgtgtta attttgtgaa taaatatcac
    22621 gacaggagtt aatcaaatgg atgcactcga actattgatc aatcgccgta gcgcctcccg
    22681 cctggctgaa cccgcaccaa cgggtgaaca actgcaaaac atcctgcgtg cgggtatgcg
    22741 cgcgccggac cataagtcca tgcaaccgtg gcattttttt gtgattgaag gggaagggcg
    22801 cgagcgtttc agcgccgtac tggaacaggg ggcgattgct gccggtagtg atgacaaagc
    22861 tatcgacaaa gcccgtaatg cgccgttccg cgcaccgctc atcatcacgg tggtggcgaa
    22921 atgcgaagaa aatcataaag tcccgcgatg ggaacaggaa ttgtctgccg gatgcgcggt
    22981 catggcgatg caaatggcag ccgttgccca ggggtttggc ggcatctggc gcagtggcgc
    23041 attaactgaa agtccggtag tgcgtgaagc attcggttgc cgtgagcagg ataaaattgt
    23101 cggttttctc tacctcggta cgccgcagct gaaagcatct acgtcgatta acgtcccgga
    23161 cccgacgccg tttgtcactt atttctgata attgtcgcga aactgtctgg attgtgagca
    23221 aaggcgcagg aattcagaca ctctcactta tcacttcacg gaatgagggt taccataccc
    23281 ggattgcatt gacaggagat gtccatgagc gagaactcga ttcgtttgac ccaatacagc
    23341 cacggagctg gttgcggctg taaaatttcc ccaaaagtgt tggaaaccat cctgcacagt
    23401 gagcaggcga agtttgttga tccgaatttg cttgtgggta atgaaacccg cgacgatgcg
    23461 gcggtgtacg atctgggcaa tggcaccagc gttatcagta ccaccgactt ctttatgccg
    23521 atcgttgata atcctttcga ttttggccgc attgcggcga ctaacgccat cagcgatatc
    23581 ttcgctatgg ggggcaaacc gattatggcg attgcgatcc tcggctggcc gattaacaaa
    23641 ctttccccag aaattgcccg cgaagtaacc gaaggtggac gctatgcatg ccgtcaggcg
    23701 ggtattgcgc tggctggcgg tcactccatc gatgcgccgg agccgatttt tggtctggcg
    23761 gtgacgggga tcgtaccgac cgagcgggtg aagaaaaaca gtaccgcaca agccggatgt
    23821 aaactgttcc tgacgaaacc gctggggatc ggcgttctta ccacggctga gaaaaaatca
    23881 ctgttgaaac cagaacatca gggactggcg acggaagtga tgtgccggat gaacatcgca
    23941 ggtgcgtcct ttgccaacat cgaaggcgta aaagcgatga ccgacgttac tggttttggt
    24001 ctgctgggcc atttgagcga aatgtgtcag ggcgcaggtg tgcaggcgcg cgttgactat
    24061 gacgcgatcc caaaattgcc gggggttgaa gagtacatta agttgggcgc agtgcctggc
    24121 ggcaccgaac gtaactttgc cagctacggt catctgatgg gtgaaatgcc gcgtgaagtg
    24181 cgcgatttgc tgtgcgatcc gcaaacttct ggcggtttgc tgctggcggt catgccggaa
    24241 gcagaaaatg aggttaaagc tacagccgcc gagtttggca ttgaactgac ggcgattggc
    24301 gaactggtgc cagcgcgcgg cggtcgtgcc atggttgaga tccgttaatt caatgcggtt
    24361 gtttattgcc gaaaaaccga gtctggcgcg cgccattgct gatgtcctgc ccaaaccgca
    24421 ccggaaaggc gatggcttta tcgagtgcgg taatggtcag gtggtgacct ggtgtatcgg
    24481 tcacctgctt gagcaggcgc agccagacgc ctacgacagc cgctatgcgc gctggaatct
    24541 tgcggatttg ccgattgtcc cggaaaagtg gcaattacag ccccgaccct ccgtgaccaa
    24601 acaacttaac gtcatcaaac ggtacctgca tgaagccagc gaaatcgttc acgccgggga
    24661 cccggatcgc gaagggcaac tgctggtgga tgaagtgctg gactatctac aactggcacc
    24721 ggaaaagcgc cagcaggtac agcgttgctt gataaacgac ctgaacccgc aggcggttga
    24781 gcgggcgatc gaccgtcttc gttccaacag tgagtttgta ccgctgtgcg tttctgcgct
    24841 ggcgcgagcg cgtgccgact ggctgtacgg catcaatatg acccgtgcgt ataccattct
    24901 cggtcgcaat gccggttatc agggcgtact ttccgtggga cgcgtgcaga cgcccgtgct
    24961 tgggctggtg gtgcgccgcg atgaagagat tgaaaacttc gtggcgaaag acttctttga
    25021 agtcaaagcg catatcgtga cacctgccga tgagcggttt accgctatct ggcaaccgag
    25081 cgaagcgtgt gaaccgtacc aggatgaaga agggcgcttg ttacatcgtc cactggcgga
    25141 gcatgtggtt aaccgcatta gtggtcaacc ggctatagtc actagctata acgataaacg
    25201 ggaatcagaa tccgcgccgc tgcctttttc gctttcggcg ttgcagattg aagcggcaaa
    25261 acgttttggt ctgagtgcgc agaacgtgct tgatatctgc cagaagctgt acgaaacaca
    25321 caagctaatc acttatccgc gttccgattg tcgctatttg ccagaagaac attttgccgg
    25381 acgccacgcg gtgatgaatg ccatcagcgt tcatgcacca gatctgttgc cgcagccagt
    25441 ggtagatcca gatatacgca accgctgttg ggatgacaaa aaggtcgatg cgcaccacgc
    25501 catcatcccg accgcacgga gttctgcgat caacctgacg gagaacgaag cgaaggtcta
    25561 taacctgatt gcccgtcagt acttgatgca gttcagcccg gatgcggtgt tccgcaagtg
    25621 tgttatcgaa ctggacattg ccaaaggcaa atttgtcgct aaagcgcgtt ttcttgctga
    25681 agcaggctgg cgcacgctgt taggcagcaa agagcgcgat gaagaaaacg acggcacgcc
    25741 attgcctgtg gtggcgaaag gcgatgagtt gctgtgtgaa aaaggtgaag tggtagagcg
    25801 gcaaacccag ccgcctcgcc attttaccga tgcaacactg ctttcggcga tgaccgggat
    25861 cgcgcgcttt gtgcaggaca aagatctgaa aaagatcctt cgtgcgaccg atggtctggg
    25921 gacagaagca acgcgtgccg ggattattga actgttgttc aagcgtggtt tcctcactaa
    25981 aaaagggcgc tatatccact ccaccgacgc cggaaaagcg ttattccatt cgctgccaga
    26041 aatggctacg cgaccggaca tgaccgcgca ctgggaatcg gtgctgacgc aaatcagcga
    26101 aaagcagtgt cgctatcagg actttatgca gccgctggtg gggacgctat atcagcttat
    26161 tgatcaagcc aaacgtactc cggtgcggca gtttcgcggc attgtggctc cgggcagtgg
    26221 tggcagtgct gataagaaaa aggctgcacc gcgtaaacgt agtgcgaaaa aaagtccgcc
    26281 agcagatgaa gccggaagcg gggcgatagc gtaagcgagc gaatctttcg tgctattcga
    26341 gtcatattct gaaatatcca gcggatcaag aaaattcgtt ggatattttt ttgcatggat
    26401 aaaattatcg cctctaaagt atgtaataac agggaatgtg atgaaaaaag t
//
LOCUS       DAZGBC010000060        26225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGBC010000060 DAZGBC010000000
VERSION     DAZGBC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.060"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..377
                     /gene="bhsA"
                     /locus_tag="ACHK1J_002395"
                     /gene_synonym="ycfR"
     CDS             120..377
                     /gene="bhsA"
                     /locus_tag="ACHK1J_002395"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO7911646.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(459..1421)
                     /gene="ldtC"
                     /locus_tag="ACHK1J_002396"
     CDS             complement(459..1421)
                     /gene="ldtC"
                     /locus_tag="ACHK1J_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO7911647.1"
                     /translation="MMIKTRFSRWLTFFTFATAVALALPVKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1565..5011)
                     /gene="mfd"
                     /locus_tag="ACHK1J_002397"
     CDS             complement(1565..5011)
                     /gene="mfd"
                     /locus_tag="ACHK1J_002397"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO7911648.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEVINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQNDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREVLRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5139..6212)
                     /locus_tag="ACHK1J_002398"
     CDS             complement(5139..6212)
                     /locus_tag="ACHK1J_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO7911649.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVLQW
                     LALSALNQWLAPERDLSNASNAAYADSTGGFLHGMITASTSLWYLYALIVYFVLCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6474..7673
                     /gene="lolC"
                     /locus_tag="ACHK1J_002399"
     CDS             6474..7673
                     /gene="lolC"
                     /locus_tag="ACHK1J_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO7911650.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRFPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWHDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7666..8367
                     /gene="lolD"
                     /locus_tag="ACHK1J_002400"
     CDS             7666..8367
                     /gene="lolD"
                     /locus_tag="ACHK1J_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO7911651.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8367..9611
                     /gene="lolE"
                     /locus_tag="ACHK1J_002401"
     CDS             8367..9611
                     /gene="lolE"
                     /locus_tag="ACHK1J_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO7911652.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9640..10551
                     /gene="nagK"
                     /locus_tag="ACHK1J_002402"
     CDS             9640..10551
                     /gene="nagK"
                     /locus_tag="ACHK1J_002402"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO7911653.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10567..11388
                     /gene="cobB"
                     /locus_tag="ACHK1J_002403"
     CDS             10567..11388
                     /gene="cobB"
                     /locus_tag="ACHK1J_002403"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO7911654.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11544..12590)
                     /gene="potD"
                     /locus_tag="ACHK1J_002404"
     CDS             complement(11544..12590)
                     /gene="potD"
                     /locus_tag="ACHK1J_002404"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO7911655.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(12587..13381)
                     /gene="potC"
                     /locus_tag="ACHK1J_002405"
     CDS             complement(12587..13381)
                     /gene="potC"
                     /locus_tag="ACHK1J_002405"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO7911656.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(13378..14235)
                     /gene="potB"
                     /locus_tag="ACHK1J_002406"
     CDS             complement(13378..14235)
                     /gene="potB"
                     /locus_tag="ACHK1J_002406"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO7911657.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(14219..15355)
                     /gene="potA"
                     /locus_tag="ACHK1J_002407"
     CDS             complement(14219..15355)
                     /gene="potA"
                     /locus_tag="ACHK1J_002407"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO7911658.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15605..16831
                     /gene="pepT"
                     /locus_tag="ACHK1J_002408"
     CDS             15605..16831
                     /gene="pepT"
                     /locus_tag="ACHK1J_002408"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO7911659.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(16880..18001)
                     /gene="roxA"
                     /locus_tag="ACHK1J_002409"
                     /gene_synonym="ycfD"
     CDS             complement(16880..18001)
                     /gene="roxA"
                     /locus_tag="ACHK1J_002409"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO7911660.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(18077..19537)
                     /gene="phoQ"
                     /locus_tag="ACHK1J_002410"
     CDS             complement(18077..19537)
                     /gene="phoQ"
                     /locus_tag="ACHK1J_002410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO7911661.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(19537..20208)
                     /gene="phoP"
                     /locus_tag="ACHK1J_002411"
     CDS             complement(19537..20208)
                     /gene="phoP"
                     /locus_tag="ACHK1J_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO7911662.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(20376..21746)
                     /gene="purB"
                     /locus_tag="ACHK1J_002412"
     CDS             complement(20376..21746)
                     /gene="purB"
                     /locus_tag="ACHK1J_002412"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO7911663.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     ASYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(21750..22391)
                     /gene="hflD"
                     /locus_tag="ACHK1J_002413"
     CDS             complement(21750..22391)
                     /gene="hflD"
                     /locus_tag="ACHK1J_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO7911664.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(22427..23533)
                     /gene="mnmA"
                     /locus_tag="ACHK1J_002414"
                     /gene_synonym="trmU"
     CDS             complement(22427..23533)
                     /gene="mnmA"
                     /locus_tag="ACHK1J_002414"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO7911665.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRVDVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(23587..24048)
                     /gene="nudJ"
                     /locus_tag="ACHK1J_002415"
     CDS             complement(23587..24048)
                     /gene="nudJ"
                     /locus_tag="ACHK1J_002415"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO7911666.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(24058..24711)
                     /gene="rluE"
                     /locus_tag="ACHK1J_002416"
     CDS             complement(24058..24711)
                     /gene="rluE"
                     /locus_tag="ACHK1J_002416"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO7911667.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            24883..26133
                     /gene="icd"
                     /locus_tag="ACHK1J_002417"
     CDS             24883..26133
                     /gene="icd"
                     /locus_tag="ACHK1J_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO7911668.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         6344 a         6678 c         6861 g         6342 t
ORIGIN      
        1 aattaattaa tgattgttat aaaaaatatc ttgtatgtga tccagatcac atctatcatt
       61 tagttatcga tcgttaagta attgcttgcg acgtcattca tctgcataag gccactatta
      121 tgaaaaacgt aaaaactctc atcgctgcgg cgattttaag ctccatgtca tttgccagct
      181 ttgcggctgt cgaagttcag tcaacgccag aaggccaaca aaaagtcggt acaatcagtg
      241 ctaacgcggg gacaaatctg ggatcgctgg aagagcagct ggcgcaaaaa gcggatgaga
      301 tgggcgcaaa atctttccgt attacttctg taaccggtcc gaataccctc catggaacag
      361 cagtaattta taaataagca ttaaccctca ttaatgcctg ctactgctga ttttttcccc
      421 gcgacatgcc gtgtcgcggg gattttttta tccaggattt acagagtttg tgggctcact
      481 tgatgacggc ggacatccac cggcatcccg gaacggacat ccatcacatg ttgcatcact
      541 tcagcgtcag tttgtgctgc atctttaaat gattgcattg cgctattcag cgtaattggc
      601 agcagctgcg gatcgtcatc aatcttctct gacagcggct gatgtacttc aaccagacgc
      661 gcaccgtttg gttcggcaga gacttttatc ggagtgttga tgatattcac tttggtgcct
      721 ggggtgacct ggctaaagag tgttttgata tcgtcatccc gcagacgaat acagccagaa
      781 cttacccgca tgccaatgcc gaaatcggcg ttcgtaccgt gaagcaaata aacgccgcca
      841 taggccgcca gacgaatcgc atgatggccc attgggttat ccggtccagc cggcactacc
      901 gcaggcaact caattccctg tgccttatac cgcgcgcgga tgtttgccgt tggcgtccag
      961 gttgggtttg ctcgtttgtc tgaaacggtg gtcaccattg tcggtgtcag cgtgtcacca
     1021 cccaactgac caatacctat tggatacacg gttaccgaat ttttacccgg cgggtagtaa
     1081 tagagacgca gctccgcaat attgatcaca atgccttcgc gcggcgcatc tggaagtagg
     1141 gtttgcaacg ggatcgttaa cacgctgccc gcgcgcggta cgtaaggatc aacgccgggg
     1201 ttagcctgta acagagcgag aaagccgacg ttatattttt tggcgatggc ttccagagaa
     1261 ccaccgtcat tttccaccac atgaaatttg ttttcgccaa ccagacgact gcccgctggc
     1321 ggcagcggcc aggtgttggc ttttaccggt agcgccagcg ccacggcagt ggcgaacgta
     1381 aaaaacgtta gccagcgaga aaaacgcgtt ttgatcatca ccaaaaatcc ataataaata
     1441 taaggttatt gtaataaaca gataagcctg aattatggat ggtgacagtg tcggatagtg
     1501 cagggaagtg caaagaattt gtaaatgttg cagatggggg cgcagaaacg cccccgattt
     1561 accattaagc gatcgcgttc tcttccagtt cacgcataaa ctggcgtacc cattcgatac
     1621 gcgttttccg ctcactcaaa tcctgaataa atttcaggcg cgtcggacca tcaaggcggt
     1681 aatgctgcgg ctgtttttgc agcaaaccaa tcaaccaggc cggattaacg tgattcttct
     1741 cggcaaattc gatcacgccg cctttctcat taccttccag cttcctgatc cccagtttct
     1801 gcgcttgctg gcgcagacgg gcaatatcca gcagggtacg cgccggatcc ggcagcaggc
     1861 cgaagcgatc gataagctcg actttgatct cttccagttc gttttccgtt ttggcgctgg
     1921 caatacgttt gtagaacgac agacgcgtgt tcacgtcagg gatgaaatca tctggcaata
     1981 gcgacggcat ccgcagctcg acttctgttt gctggctggt gagatcttcc agcgacggct
     2041 cgcgtccggc tttcagtgca tcgacggcgt tttccagcaa ctccatatac agcgagaaac
     2101 cgatggtttc cattgagccg ctttgttctt cgccaagcag ttcacccgcg ccgcggatct
     2161 ccagatcgtg cgttgccagc gcaaaacctg ccccgagatc ttccagcgag gcaatcgctt
     2221 caagacgttt ttgcgcatcg gtagtcatcg cttttggatg cggcgtcagc agccatgcat
     2281 atgcctgatg atgtgaacgt ccgacgcgac cgcgcaactg gtgcagctgc gccagaccga
     2341 agtgatccgc gcgttcaatg ataatggtgt tggcggtagg gatatcgatt ccggtttcga
     2401 taatggtggt acaaaccagc acgttgaaac gttgatgatg gaaatcattc atcacccgtt
     2461 ccagttcgcg ctcgcgcatc tgcccgtgac cgatggcaat ccgtgcttct ggcaccagtt
     2521 ctgccagccg ttcggcagct ttctggatgt tttccacatc attgtagaga taataaacct
     2581 gaccgccgcg cagaacttca cgcaggatcg cctcccggac caccagactg tcatactcgc
     2641 ggacaaacgt tttgaccgcc agacgacggg cgggcggcgt ggcgataatc gacaggtcac
     2701 gcattccgct catcgccata ttcagcgtac gtgggatcgg cgtagcggta agcgtcagaa
     2761 tatccacgtt cgcacgcatc gctttaatgc gctctttatg acgcaccccg aagcggtgtt
     2821 cttcatcgac aatcagcaga cctaaatctt taaacttgac gtcattttgc agcagtttgt
     2881 gcgtaccgat cagaatatcg attttccctt ccgccacttc cgcaaggatt tgcgtttgct
     2941 ctttggcgct gcggaaacgg gagatcattt cgatacgtac cggccagttg gcgaaacggt
     3001 cgcggaagtt gtcgtaatgc tgctgcgcga gaagggtggt aggcaccagc accgctacct
     3061 gcttgtggtt atctactgcc aggaaagcgg cgcgcatcgc cacttctgtt ttcccaaagc
     3121 caacatcgcc gcacaccaga cgatccattg ccagcggctg acacatgtcg ctaagcaccg
     3181 cattaatggc ctgcgcctga tccggcgtgg tttcaaacgg gaagctgtcg cagaacaact
     3241 gatactgctc acgatcgtgt ttaaacgcga agccctcttt ggcggcgcgt tgcgcgtaga
     3301 tatccagcaa ttctgccgcc acatcacgca ctttttccgc cgctttctgc cgcgcgcgtg
     3361 accacgcatc gccgccaagt ttatgcagtg gggcgttttc ttccgcgcca cctgcgtaac
     3421 ggctaatcag atgcagtgac gacaccggaa catacaattt ggcgtcgttg gcataggtga
     3481 gcatcaggta ctcgccggta atgccgccag cttccagcgt ggtcattcct gcatagcgcc
     3541 cgacgccgtg ctccagatgg accaccggct gaccaatgtg caactccgcg aggttacgga
     3601 tcagtgtatc ggggttgatg gtgcggcgag agtcctgacg acggcgggca acgcgttcac
     3661 cgagcagatc gctttcgcaa atcagcgcca ggttacgcac cttatcgaca aaaccatgtt
     3721 cggcagcgcc aatcatcaaa taacgcccac ggtcgctggc ttcatcaaga cgcataatgc
     3781 gttgcggagc aattttaatt cgcgcgagca gttcacccag cgcttcacgg cgaccttcac
     3841 tttctaccga gaacaccacc ggaccgtcga aagactcgag gaacttacgc agcgcatcca
     3901 gcggcgcttt ttgttgtgcc tgaacggcca ggtctggcag tttctggaaa cctaaattgg
     3961 cattcgcggc ttttgtcggt aaatgttcag tttttagctg cactcggggc cagtttttca
     4021 gctctgagaa gagctcgtcc acccgcagcc agagcgattg tggtggcaac agtgggcgca
     4081 tcggatcgac gccgcgattc tcaaaacgcg ccagcgtgtc agcctggaaa cgttcggcac
     4141 tgttttccag atcgccagta ttcaccagca aggtattggc agggaaataa ctgaacagcg
     4201 gcggcagtgg ttcgctgaag aacaatggct gccagtactc gatcccggca ggtaatgtgc
     4261 ctttactcac ttgctggtaa atatgttccg gatcgcgctt cacttcgaag gtatcgcgcc
     4321 actggctgcg gaacagttca attgccgctt tatcggtcgg aaattcgtgc gcgggcagca
     4381 gattgatcac ttctacttcc tccagcgtgc gctggctgtc gacgtcaaac acccgcaggc
     4441 tgtcgatttc atcatcaaag aaatcaagac gataaggcag ctcactcccc atcgggaaga
     4501 gatccagcaa cgcgccgcgc gtggcgtatt cgccgtgctc catcacctgg tcaacatggc
     4561 gataaccggc gctgtccagt tgggttcgta atgcatctcg tgacaggcgc tgaccttttt
     4621 tcatcaccag cgcatgaccg tggagaaaac tgtgtgggca aacgcgctgc ataagcgtat
     4681 tcaccggaac aatcagtacg ccacgctgca tcgtcggtag ctggtaaagg gtggaaaggc
     4741 gcgaggagat aatgtcctga tgaggcgaaa aactgtcgta agggagggtt tcccagtccg
     4801 ccagattcat caccatctga tcggtgaact ggctgatttc atcatgcaaa cgcagagcat
     4861 tttgcatatc tggtgcaatg agtaccaccg gaccggcgtg acgttcggca atttccgcca
     4921 ccagcgtcgc gcaggcagcg ccggttaact cgcccagcag acgctgctca cccgctttga
     4981 cgggcagcgt ataacgatat tgttcaggca taacggttgt cagattctcg ttaggatatg
     5041 cctcaacata tgggggcata tctctgataa gcaatgctgt tcattatccg taatcgacat
     5101 gctttagcaa atcgaatcgc cccggcgggg gcgatggctt agcgggagta actgacggca
     5161 gcgggcagtg aaggcgggga gaacaacaga tcgctgaatg cacgctgtga aagttttctt
     5221 accagcaagc ccgcaacagt acagatgaac aaactgacaa agggataaac cagcaggagt
     5281 gtcagttcga cttgcggcga ccagcgtgct gcgttcattt gcgcaagcag agttaagctg
     5341 aatatttcaa ccagaatgcg atgggtggtg tagatagcaa tggtgttgga gccaatcaca
     5401 ttcagcaggc tggtggagcg cataccgaat cgttgctcgt attgataaaa cagcttcatg
     5461 atcaccacaa tcgataccag cgacaacagc agcgagatag taaacaacca ggcaccgacc
     5521 gccagaacgg tcagcaaaga agccatcagc agatggcggc gcaacggcac ctctttaaca
     5581 caggtcatta ttgttgcgcc aaaccatgcg ccaaggctgt aataaggcaa attgcggatc
     5641 acactgttca ttccccacca cggcgtggga acgaaattaa ccgccacact cagcagtaca
     5701 aacaaggcga atagtggcag cgccagacgg ctaaaaattt tacataacac gaaatagaca
     5761 attaacgcat acagatacca caagctggtg ctggcggtga tcatcccgtg caggaaccca
     5821 ccggtggaat cggcataagc ggcattggag gcattgctta aatcgcgctc aggtgccagc
     5881 cactggttca gcgcacttaa cgccagccac tgcagcacgc cccaaagcgc cagcacccag
     5941 aagatgttcc agatccgttt atcgagacaa tttccccacg gtacgctgtc gatatagcgg
     6001 cgaatcaaat agccggaaat aaagaaaaaa accggcatac gaaagggggc aaggtaaaga
     6061 ttgaaataga tccagcattt gctcaggact tccgataacg gatgctgaaa agtggtcaga
     6121 tgcggataaa aggtaatgac cgagtgataa atcacgacca gacaaataca taaccctttg
     6181 atctggttaa tccatagctc tttttgtttc atcaggacaa cacgctcttt tttaatttac
     6241 atgcggttga tcctgcgggt tatgacccga ggtgtaatgg gtatctgtct gtattggatg
     6301 atttttcaga ttaagatcag gcggcaagat tgatgataaa acatggcaat ttagccgatt
     6361 gatttacggg ggcttttcag attagccctg acgatcactt acagttcaga cgtttaccca
     6421 tcctgctttc gcttatatac tcgtgtcttt gctacagcaa ccagacggat ttcatgtacc
     6481 aacctgtcgc tctatttatt ggcctgcgtt acatgcgtgg gcgtgcagcg gatcgcttcg
     6541 gtcgtttcgt ctcctggctt tctaccatcg gcattaccct cggggtgatg gcgctggtca
     6601 cagtattgtc agtgatgaac ggctttgagc gcgagctgca aaacaacatc cttggcctga
     6661 tgccacaggc aattctttct tctgagcatg gctctcttaa cccgcagcaa ctcccggaaa
     6721 cggcagtcaa actggacggc gttaatcgcg ttgcacctat tactaccggt gatgtggtac
     6781 tgcaaagcgc gcgcagcgtg gcggtcgggg tgatgctggg tatcgacccg gcgcaaaaag
     6841 atccactaac gccgtatctg gtcaatgtga aacaaactga cctcgagccg gggaaatata
     6901 atgttatcct cggtgaacag cttgcctcac agttgggcgt caatcgcggc gatcaaatcc
     6961 gcgtgatggt gccatctgcc agccagttca cgccaatggg gcgtattcca agccagcgcc
     7021 tgttcaatgt gattggcact ttcgccgcca acagtgaagt cgatggctat gaaatgctgg
     7081 tgaatattga ggatgcctcg cgtctgatgc gttttccggc aggcaatatt accggctggc
     7141 gtttgtggct ggatgagccg ctgaaagttg actctttaag tcagcaaaaa ctgcctgaag
     7201 gcagcaaatg gcatgactgg cgtgaccgta aaggcgagct gttccaggcc gtacgcatgg
     7261 aaaaaaatat gatgggctta ctgctgagcc tgattgtcgc cgttgcggcg tttaacatta
     7321 ttacctcgct ggggctgatg gtgatggaga agcagggcga agtagcgatc ctgcaaacgc
     7381 aaggcttaac tccgcgacaa atcatgatgg tctttatggt gcaaggggcc agcgccggga
     7441 ttatcggtgc gatcctcgga gcggcgcttg gcgcactgct tgccagccag ttaaataatc
     7501 tgatgccgat aatcggcgtc ctgcttgatg gcgcggcgct gccggtggct atcgaacctt
     7561 tacaggtcat tgttattgcg ctggtggcga tggctatcgc gctgctgtct acgctttacc
     7621 cttcatggcg cgctgccgcc acacaacccg ctgaggcttt acgttatgaa taagatcctg
     7681 ttgcaatgcg acaacctgtg caaacgctat caggaaggca gtgtgcaaac cgatgtgctg
     7741 cacaatgtca gtttcagcgt gggcgaaggt gaaatgatgg caattgtcgg tagctctggt
     7801 tccggtaaaa gtaccttgct gcacctgctg ggcgggctgg atacaccaac ctccggcgat
     7861 gtgatcttta acggtcaacc gatgagcaaa ctgtcttcgg cggcgaaagc agagctgcgc
     7921 aaccagaagc tgggctttat ttatcagttt caccacctgc tgccggattt taccgccctg
     7981 gaaaacgtgg ctatgccgct gctgattggc aagaaaaagc ccgctgaaat taacagccgt
     8041 gcgctggaga tgttaaaagc ggtggggctg gagcatcgtg cgaatcaccg cccatctgaa
     8101 ctttctggcg gcgaacgcca gcgtgtggcg attgcccgtg cgctggtcaa taacccgcgt
     8161 ctggtactgg cggatgaacc taccggtaac ctcgatgcgc gtaacgccga cagcatcttc
     8221 cagttgctcg gagaattgaa tcggttgcag ggcactgcct tcctggtggt tactcacgac
     8281 ctgcaactgg cgaaacgtat gagccgccag ttagaaatgc gtgatggtcg tttgacggca
     8341 gaactaagcc tgatgggggc ggagtaatgg cgatgccttt atcgttatta attggcctgc
     8401 gttttagtcg cggacggcga cgcggcggca tggtgtcgct gatctccgtt atttctacca
     8461 ttggcattgc cctcggcgtg gcagtattga tcgtcggctt aagcgcgatg aacggctttg
     8521 aacgcgaact gaataaccgc attctggcgg tggtgccgca tggcgaaata gaggcggtgg
     8581 atcaaccgtg gactaactgg caggaagcac tggataacgt gcaaaaagtg ccaggtattg
     8641 ccgccgctgc gccgtatatc aatttcaccg ggctggtgga aagtggcgcg aatctgcgcg
     8701 caatccaggt gaagggcgtt aacccgcaac aggaacagcg tctgagcgca ttaccctcgt
     8761 ttgttcaggg ggatgcctgg cgcaatttta aagcgggcga acagcaaatt atcatcggca
     8821 aaggcgtggc ggatgcgctg aaagtgaagc agggcgattg ggtgtcgatt atgatcccca
     8881 actcgaatcc cgagcataaa ctgatgcagc caaaacgtgt gcgtttgcac gttgccggta
     8941 ttttgcagtt gagtggtcaa ctcgatcaca gttttgccat gatcccgctg gcggatgccc
     9001 aacaatatct tgatatgggt tccagcgtgt caggtattgc ccttaaaatg acggatgttt
     9061 tcaacgccaa taagctggta cgcgatgcgg gtgaagtgac caacagctat gtttatatta
     9121 aaagttggat tggtacttac ggctatatgt atcgcgatat ccagatgatc cgcgccatta
     9181 tgtatctggc gatggtactg gtgattggcg tggcctgttt caacatcgtc tccaccttag
     9241 tgatggcggt gaaagacaag agtggcgata tcgcagtatt aagaacgctg ggggcgaaag
     9301 atggtttaat tcgcgccatc tttgtctggt atggattgct ggcagggctg ttcggcagcc
     9361 tgtgtggggt gattatcggc gtggtggttt cactgcaact taccccgatt attgagcgga
     9421 ttgaaaagct gatcggtcat cagttcctct ccagcgatat ctattttatt gacttcctgc
     9481 catcggaatt gcactggctg gacgtcttct acgtactggt cacagcattg ttgctgagtc
     9541 ttttggcaag ttggtatccg gcgcggcgcg ccagtaatat tgaccctgcg cgagtcctta
     9601 gcggccagta aaggcagtac attaaaacaa ggagcggcaa tgtattacgg gtttgatatt
     9661 ggtggaacaa aaattgcgct tggcgtgttt gatagcggtc ggcagttgca gtgggaaaag
     9721 cgggtgccga caccgcgtga cagctatgac gcatttttag atgcagtgtg tgagctggta
     9781 gctgaagctg accaacgttt tggctgtaaa ggttctgtcg gcatcggtat tccgggaatg
     9841 ccggaaacag aagatggtac gctgtatgcc gccaatgtcc ccgctgccag cggtaaaccg
     9901 ctgcgtgccg acctgagcgc acgtcttgat cgcgatgtcc gccttgataa cgatgctaac
     9961 tgttttgccc tttcagaagc ctgggatgat gaatttactc aatatccact ggtgatgggg
    10021 ttgattctcg gcaccggcgt tggcggcggg ctgattttca acggtaaacc gattaccggt
    10081 aaaagctata ttaccggcga gtttggccat atgcgtctgc cggttgatgc gttaaccatg
    10141 atggggttgg atttcccgtt acgccgctgc ggctgtggtc agcatggctg cattgaaaat
    10201 tatctgtctg gtcgcggttt tgcgtggctg tatcaacact attatcatca accgttgcag
    10261 gctcccgaaa ttattgcgct ttatgatcaa ggcgatgagc aggcaagggc gcacgttgag
    10321 cgttatctgg atttattagc ggtttgtctg ggaaatatcc tgaccattgt tgaccctgac
    10381 ctggtcgtca ttggtggtgg cttatcgaat ttcccggcaa tcacaacgca actggcggac
    10441 aggctgcctc gtcatctctt acctgtagct cgtgttccgc gcattgaacg cgcgcgccac
    10501 ggtgacgcgg gaggaatgcg tggtgcggcc ttcctacatc taaccgatta aacaacagag
    10561 gttgctatgc tgtcgcgtcg gggtcatcgg ttaagtcgtt ttcgtaaaaa taaacgccgc
    10621 ctgcgcgagc gtttgcgtca gcgtattttt ttcagagata aagtggtgcc ggaagcaatg
    10681 gaaaaaccaa gagtactcgt actgacaggg gcagggattt ctgcggaatc aggtattcgt
    10741 acctttcgcg ccgcagatgg cctgtgggaa gaacatcggg ttgaagatgt ggcaacgccg
    10801 gaaggtttcg atcgcgatcc tgaactggtg caagcgtttt ataacgcccg tcgtcgacag
    10861 ctacagcagc cagaaattca gcctaacgcc gcgcatcttg cgctggctaa actgcaagat
    10921 gctctcggcg atcgcttttt gctggtgacg cagaatatag acaacctgca tgaacgcgca
    10981 ggtaatacca atgtgattca tatgcatggg gaactgctga aagttcgttg ttcacaaagt
    11041 ggtcaggttc tcgactggac aggagacgtt accccagaag ataaatgcca ttgctgccag
    11101 ttcccggcac ccttgcgccc gcacgtagtg tggtttggcg aaatgccact cggcatggat
    11161 gaaatttata tggcgttgtc gatggccgat attttcattg ccattggtac ttcggggcat
    11221 gtttatccgg cggctgggtt tgttcacgaa gcgaaactgc atggcgcgca caccgtggaa
    11281 ctgaatcttg aaccgagtca ggttggtaat gaatttgccg agaaatatta cggcccggca
    11341 agccaggtgg tgccagaatt tgttgaaaag ttgctgaagg gattataaag caggaagcat
    11401 tgcctgatgc gctacgctta tcaggcctac aagcggacca caataccgac gaaagagcgg
    11461 gataaaactt tactgtcctg actcattagc cacatccttg ctaactaaaa aacgggcggt
    11521 aataccaccg cccgcttgct gaattaacgt cctgctttca gcttctgata atactcttca
    11581 taaatgctgc tggctgcgcc aacgtcattc tgccattcgc catttttaat ggtttcagca
    11641 tccgggtaga gcgtcttatc gttcgccact tctggactta acagcttacg cgccgcaagg
    11701 tttggcgttg gataaccgat agtttcagca acctgttttg ccacatccgg gcgcagcagg
    11761 aagttgatca atttcagcgc gccttctttg tttttggcat ttgccgggat cgccaggctg
    11821 tccatccaga atatgccgcc ttctttcggc cacaccacgt caataggcgt acccgcctgg
    11881 cgtgcaacga aagcagaacc gttccagatc atgccgaggt taacttcgcc ttccatgtac
    11941 gggttagccg ggttatcgga gttaaacgct gcgacgtttg gcatcagttt tttcagctcg
    12001 ttatatgcag cttcaatctc tttcggatcg gtggtgttac cggagtagcc cagcttacgc
    12061 agcgccatct ggaacacttc acgggcatcg tcggtcagca gcaggctgcc tttgtactca
    12121 ggcttccaca gatcggccca gctggtaacg gatttcggat ctaccgcatc accgttaacg
    12181 ccaatcgccg tcgcccccca gatatacgga atggagtagt cgttattcgg gtcaaacggc
    12241 ttgttgagca tgtctggatc gaggttgctg aaattgctta acttcgactt gtcgatcttc
    12301 tggatcatcc cttctttacg cattttatcg acgtaatagg ttgaaggaac caccaggtcg
    12361 taggcaccgt ctttgtacgt tttcagcttg gcgtacatgg tttcgttcga ctcgtaagtc
    12421 gaatagataa ccttaatacc ggtttctttg gtgaactgtt caagcagtcc tggcggcacg
    12481 tactcggtcc agttgtagaa atacagcgtg ttgttgtcat cggcgtgagc ggcgctcatg
    12541 cccagtgcca gagcacccgc cgcgagcagg tggcgtgacc attttttcat ttaacgtccc
    12601 ctgtgttacc tttcgtttta tcacgagcaa taagctggct ggcaataacc atcaccagcg
    12661 acagcaccag taatatggtt gccagcgcgt taacttccgg cgatacgcca actttgacca
    12721 tcgaataaat ttttaacggc agaatttcat aacttggtcc ggtgacgaac gaagaaacta
    12781 ccacatcgtc catcgacagg gtaaagctta acacccagcc cgccgccacc gctggcattg
    12841 ccagtggcag aatgattttt cgcagaatgg taaattcgct ggcaccgaga tctttcgccg
    12901 cttccagcat tcgcacgtca aaacctttca ggcgcgaata caccgtcacc accacaaatg
    12961 gcaggcagaa ggtgatatgc gagaacagca gcgaccagaa gccaagctga atacccagca
    13021 gcataaacag caccagcagc gaaatcgcca tcacgatatc tggtgacatc atcaccacaa
    13081 acagcattcc gctaacgaac ggcttaccac gaaagcgata acggtacagc gcaactgccg
    13141 tcagtgaacc gataagcgta gcaaacgtcg ccgaaaacac cgccattgtc agtgaatgct
    13201 gcgctgcctg taacaggctg tcgttgttca tcagcaagct ataccattta gtggtaaaac
    13261 cctgccagtt gatgccaaag cgcgagctgt taaaggagtt cacaatcaaa ataatgattg
    13321 ggatatacag gtacgcgtag atagcggtca taaaaccgcc gcgaagcagt cgaccgatca
    13381 ttcgagttcc accttcttat tcagcagacg agaagcgcgc cagtaaacca gcaacatcag
    13441 gcccattacg atagtcagcg taatgctggt ggctgcacca aacggccagt cacgaatatt
    13501 aaggaactgg accttgatga cgttaccgat cagcaggttt ttcgcaccgc ccatcaggtc
    13561 ggacacatag aacaggccca tcgctggcag catcaccagc agacatccgg caataatccc
    13621 cggcatcgtc agtggaataa tgatacggat aaaagtctgt aacttgctgg caccgagatc
    13681 gcgcgccgcc tcaagcaacg gcttatccag tttttcgata ctggagtaca gcggcatcac
    13741 cataaacggc agcagaatgt aaaccagacc gataatcacc gcactgggcg tgaacatgat
    13801 acggattggt gtgtcgataa cgcccagcca gagcaaaaac tcgttgaggt agcctttggt
    13861 gctgaggaaa attttcagcc cgtagatgcg aatcagtgag ttggtccaga agggaacaat
    13921 cagcagaaac agcagcagcg gacgcacctt gtgtggcagc ttcgccagaa accaggcaaa
    13981 cggatagccc agcaccaggc aggcgagggt ggcgatcagc gccatattca gcgagtgcaa
    14041 tagcacttca aaatagagcg gatcgagcag acgtgtgtag ttatccagcg taaagaccat
    14101 tttgacgaaa ctggcgtcgt cgcgggtcaa aaagctggtg ccaatgatca tcaggttggg
    14161 cagaaagaca aacaacacaa gccaaccgac aatagtgaca atcactacat tctggaactt
    14221 acttgtgttc ttcatcagcc agtacgacct cccagctttc tacccaatta atggccattt
    14281 tttggtcgag agagtggtca aagtcaggat cgtcttcatt gaagaattcg ctgaccatca
    14341 ccatcttgcc attttccagt tcaacaaccg actccagcgt catgcctttg tagttacgct
    14401 cgcgaacgta accaatcagc ccttcagcgt ggttgtcgtc gttaatctct tcaacacgta
    14461 agtcttccgg gcgcagcaga acatgcagtt tttgccccgg ttcaacggcg aagttaacgt
    14521 agatattaca ttcgcggcct tcaacgttgg cgcgtacgcg ctgctcgtcg agacgttcga
    14581 tgacagtggc gttaaacata ttgatttcgc caatgaaccc ggcaacaaac aggtttttcg
    14641 gctcttcgta gatttcacgc ggcgtgccgt cttgctcaat gcgaccatcg cgcatcacca
    14701 caatcctgtc cgacatggtg agtgcttctt cctggtcgtg agtcacgaag acgaatgtaa
    14761 tgccaagctt acgctgtaac gctttcagct cgttctgcat ttgcttgcgc agtttgtaat
    14821 ccagcgctga gagcgactca tccagcaaca acagacgagg cttgttaacc accgcgcgag
    14881 caatggcgac gcgttgctgt tgaccaccag agagctgatg cggtttacgt tgagcgaagg
    14941 tttccagctg caccatccgc agggcttcca tcacgcgggg cgtaatttca gcagcggggg
    15001 ttttttgcat gcgcaaccca aaggccacat tttcgaacac ggtcatgtgg gggaaaagtg
    15061 cgtagctttg gaaaacagtg ttcacatagc ggttttccgc cggaacgtgg gtgatgtcct
    15121 cgttatccaa catgatgcgt ccggaatcaa cagtttccag acctgcaatc aggcgaagaa
    15181 cggttgtttt accgcagcca gaagggccaa gcagcgtgag gaactcgcca ttgttgatag
    15241 tcagatccag ctggggaatg acctctttac catcaaagca tttgcgaatt cccgccaatt
    15301 gcaccagcgg tgaaagcgaa ctcggttgtt tattcaattt tttactctgt cccatgtaaa
    15361 cgcaacggat ggcttaccga tgcggggttt gtggttaacc accttggtga ctcttaatga
    15421 gggcggtaat tctacggcaa accgcttgaa tcgccaatct ttgttgtgaa ttactggctt
    15481 agctttatat tcattaaggt aatgctgata aatattcccg cttgcagggg taaaagtgac
    15541 ctgacgcaat atttgtcttt tcttgcttct taataatgtt gtcacaaaaa gtgagggtga
    15601 ctacatggat aaactacttg agcgattttt gaactacgtg tctctggata cccaatcaaa
    15661 agcaggggtg agacaggttc ccagcacgga aggccaatgg aagttattgc atctgctgaa
    15721 agagcagctc gaagagatgg gccttatcaa tgtgacctta agtgagaagg gcactttgat
    15781 ggcgacgtta ccggctaacg tccctggcga tatcccggcg attggcttta tttctcatgt
    15841 ggatacctca ccggattgca gcggcaaaaa tgtgaatccg caaattgttg aaaactatcg
    15901 cggtggcgat attgcgctgg gtatcggcga tgaagtttta tcaccggtta tgttcccggt
    15961 gctgcatcag ctactgggtc agacgctgat taccaccgat ggtaaaacct tgttaggtgc
    16021 cgatgacaaa gcaggtattg cagaaatcat gaccgcgctg gcggtattgc aacagaaaaa
    16081 cattccgcat ggtgatattc gcgtcgcctt taccccggat gaagaagtgg gcaaaggggc
    16141 gaaacatttt gatgttgatg ccttcgatgc ccgctgggct tacactgttg atggtggtgg
    16201 cgtaggcgaa ctggagtttg aaaacttcaa cgccgcgtcg gtcaatatca aaattgtcgg
    16261 taacaatgtt cacccgggca cggcgaaagg agtgatggta aatgcgctgt cgctggcggc
    16321 acgtattcat gcggaagttc cggcggatga aagcccggaa atgacagaag gctatgaagg
    16381 tttctatcac ctggcgagca tgaaaggcac cgttgaacgg gccgatatgc actacatcat
    16441 ccgtgatttc gaccgtaaac agtttgaagc gcgtaaacgt aaaatgatgg agatcgccaa
    16501 aaaagtgggc aaagggttac atcctgattg ctacattgaa ttggtgattg aagacagtta
    16561 ctacaatatg cgcgagaaag tggttgagca tccgcatatt ctcgatatcg cccagcaggc
    16621 gatgcgtgac tgcgatattg aaccggaact gaaaccgatc cgcggcggta ccgacggcgc
    16681 gcagttgtcg tttatgggat taccgtgccc gaacctgttc actggcggtt acaactatca
    16741 tggtaagcat gagtttgtga ctctggaagg tatggaaaaa gcggtgcagg tgatcgtccg
    16801 tattgccgag ttaacggcgc aacggaagta agcgaaaagg gatgcggcat gtgatgccgc
    16861 atccggctta aatccaaact tacccttcga agaaccaata cccgctattg accagcgccg
    16921 cgagcatcgc gaggaatgac ggatcttcca gcgcatcgcc aaaattctcc gcggtcagcg
    16981 caatgttgct ggcgagtgca tccagtgccg gacggtgcgg ggaatcgatc ttctcaccat
    17041 tggcatacac gtcgtcgcca atgcgcaata cgcgcagacc acccaggcgc accagcacat
    17101 caccttgttt cagcgcatcg tagatttcat ccggctgata aggcggctct ggcggcgcga
    17161 tatccagttc atgacgtgac tgggatataa actccccaaa ccattgctta aagtgttccg
    17221 gctggttgat caattcgagc atcatctcac gcagtttatc catctcttgc ggcagaacat
    17281 ccgcaggatg agcgcgaggt ggaacatcag gatcgctgta gtagttgccg cccagttcac
    17341 gttgcagcac ataatcggca aatccactaa tcagttcccg cgtatttggc gcgcgaaaac
    17401 ccacggaata gttcatcgca ttttccagcg cgtagccttc atgcgggaat cctggcggaa
    17461 tataaagaat atcgccaggc tccagctctt catcgatgat ggcttcgaac ggatcgacct
    17521 gtaacagatc cgggtgcgga cagtgctgtt tcatttgcag cttttcgccc actcgccagc
    17581 gacgacgacc ggtaccctga atgataaaca cgtcgtactg atcgagatgc gggccgacgc
    17641 cgccgccagg tacagaaaaa gagatcatca gatcatcaat acgccagtcc ggtaattcac
    17701 ggaacggtcg catcagcgcg gcggtcggct catgccagtg gttcactgct tgcactaaca
    17761 atgaccagtt agtttcaccg agatgatcgt agctttcgaa cggaccgtgg ctgacctgcc
    17821 attttccatc ctggtgactg accagtcgac tgtcgacttc gctttccatc gccagacccg
    17881 ccaactcgtc tggagagatc gggtcaataa aattattaaa gccgcgtttt aacaccaccg
    17941 ggcgtttctg ccagtgacgt tcaagaaaat cgggccagtt aagagtgagt tggtattcca
    18001 tgttaagcat ccacaggctg gtatctgcaa ccgattataa cggatgctta acgtaatgcg
    18061 tgaagtatgg acatatttat tcatctttcg gcgtagaatg ctggcgacca aaaatcacct
    18121 ccatccgcgc accgccaagc atgctctctc cggcgacgat tttaccctca tattgctcgg
    18181 tgatttcgcg ggctaccgcc agccccacac cttgcccagg gcgtaaagta tcaacccgtt
    18241 gaccacggtc gaaaatgacc tctcgcttgc ttaatggaat accgggtcca tcatcctcga
    18301 ccacaatata gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat
    18361 atttacaggc attatccagc acattgccca tcacctcgac aaaatcgttc tgctcaccga
    18421 caaagctgat ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt
    18481 tgttcagcgc cgaggtgaga ttgtccagca gtggggcgac cggatgcagc tcgcggctga
    18541 gcaatgtccc gccgcgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc
    18601 ggctgatttg ctccagcatt accggctcag catcactgac gctcattttc tcactacgca
    18661 gagaacgcag cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg
    18721 tgagcgttgt acgatatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt
    18781 ttcgtaccag actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt
    18841 cttccagttc gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg
    18901 cgacccacag cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc
    18961 tccagaccat ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta
    19021 actttggcat ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg
    19081 cgtcgtcatc atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca
    19141 gcaagaggct ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc
    19201 agtcaggctg gatcatcttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc
    19261 cgttctcatc ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact
    19321 cgacatgcaa cttattgttt tcccacttcg caagggtata gaacagattg ctctcgccac
    19381 gtaacagccg aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc
    19441 cgtaggcaag cgaaagcacc agtactaccg ctgccgttgc caacagaaaa cgtacccgca
    19501 gcgagagcgg gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acagatagcc
    19561 ctggccgcga acggtggtaa tcacttcttg gggatattgt gcctgaattt ttttgcgcag
    19621 acgtcccatc agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg
    19681 gagcattaac gaatctttgc tcaccacttt gccattattg cgtatcaacg tttccataat
    19741 ggtgtattcg aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga
    19801 gagatcaacc tgaaacggtg ggagcgaaat gacctgtgaa gccaggccgc tattacgccg
    19861 cattaatgcc tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc
    19921 atcagcaccg gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac
    19981 cagaatcggc agtgaaacat cattgctacg ccagcggcga atcagtgaca gaccgtcctc
    20041 gtctggcaat ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc
    20101 ggcttctttg gcatcttctg catcatcgac ctgatgacca gcatcctgaa tctgaacttt
    20161 aaggtggtga cgtaacaacg cattgtcttc aacaaccagt acgcgcattt ttatttctcc
    20221 ctgtcttaat tattaaaata gtgtaacgcg attatgtggt tatgggggta aacattaaat
    20281 aaaccagcgg ggaggggagg taaagtgaaa aaataaaaag cggataatct taataagcag
    20341 gccggacagc atcgccatcc ggcactgata cgagtttatt tcagctcatc aaccatggtg
    20401 atggcgcgac caatgtagtt tgccggcgtc atcgctttca ggcgggcttt ctcttcttct
    20461 ggcaacgcca gaccgtcgat aaactgcttc atgccttcgg cgtcaacgcg cttaccacga
    20521 gtcagctctt tcaatttctc gtacggtttt tcgatgccat agcgacgcat aactgtctgg
    20581 attggttctg ccagcacttc ccagttgtga tccagctcat ccagcagatg gtcacggttc
    20641 acttccagtt tgctcacgcc tttcagggtg gattgatacg caatcagcgc ataaccgata
    20701 cccacgccga ggttacgcaa cacggtggag tcggtcaggt cacgctgcca gcgggaaacc
    20761 ggcagtttgc ttgccagatg ctgtaatacc gcgttggaaa ggcccagatt cccttcggag
    20821 ttctcgaagt cgatcgggtt aactttatgc ggcatggtgg aagaaccaat ctcaccggcg
    20881 atggttttct gtttgaagtg gttaagggcg atataacccc agacgtcacg gtcaaagtcg
    20941 atcagaatgg tgttgaagcg cgcaacgcaa tcaaacagtt cggcaatgta gtcgtgcggt
    21001 tcaatctggg tagtgtacgg gttccattga atacccagcg aggtgacgaa ctcttcgctg
    21061 aactgatgcc agtcaacttc cgggtaagag gcgatgtggg cgttatagtt accgaccgcg
    21121 ccgttgattt tgccgaggat ctccacctgg ttaagctggc ggtactggcg ctccatacgg
    21181 taggcgacgt ttgccatctc tttaccgatg gttgacggcg tggctggctg accgtgggta
    21241 cgagacagca gcgggatatc gcggtactga acggcgagat ctttaatgcc atcaattagt
    21301 tgacgccagt acggcaggat cacttcatca cgcgcggttt tcagcattaa tgcgtgggag
    21361 aggttattga tatcttccga agtacaggca aagtggatga attcagaaac cgcgtgcagt
    21421 tccgggatct ccgccacttt ttctttcagg aaatactcaa ccgctttaac gtcgtggtta
    21481 gtagtacgct cgatggtttt gatgcgtgcg gcatcttctt cgctgaaatt ggcgacaatt
    21541 gcatcaaggt aaccgattgc gtcggcagca aaagcaggaa cttccttgat cgctgcgtgc
    21601 gcggccagtt tttgcagcca acgtacttca acttgtacac ggaatttcag caaaccatat
    21661 tcgctgaaaa tcccgcgcag cgcgctgact ttatcgccgt agcgtccatc gacaggggaa
    21721 acggcggtca gtgaggataa ttccatagat cacaactccg gggttaaatg agcaagaatt
    21781 tgttttgcct gagtggtcag gcgattacga gaaaacatca gttgcagacg tccgccgccg
    21841 acctggtgcc agagcacggc ggcgcgaatg cctgccagca gggttgcgcg aactttcgcc
    21901 tgcacttgtg ggctttgcag tacagcaggg gagccggtga cctgaatgcg cgggccaagc
    21961 gggctaatca catcaacata gatagcagcc atcgcgctca tcagcgtttc ggactgtaaa
    22021 tcgaagtgtt cgagctggcg ttgcaggccg ttgatccggt tgcccagagt gtcgagcgca
    22081 cctttcgctg aggagagttt gcgctcaagc accatcaggc tgagtgtgta gcgggttaat
    22141 tcggcgttta agccctggcg actgctggca ttgagcacgc cgagcagggt ttccagcccg
    22201 acgcgcaggt tggcttcgct accgccaaaa accgccagcg tcgagctggg gttcatatca
    22261 ataatactgt tgagtgagac gtgtagcgca tcggcatcac aatgcccctg gtgagcgagt
    22321 tgttgcacca ggcgtgccga ctgacaaata ccggccaggg cgagggtgat gtcatagtaa
    22381 ttctttgcca cgttcactgc ttccttgttt taagtaaaga taataatcag accggcagcg
    22441 gcagacgctg ctcaataata ccgccaccga ggcacacttc accgttatag aagacggcag
    22501 actggcccgg cgtcacggcg gcaacgggct catcgaaaat cacttcaatg cgatcatcgt
    22561 ccagcgcctt gacggtgcag gggatatcgg tctggcgata gcgggttttt accgtgcaac
    22621 gcatagtacc ggtgaatggt tcacgatcga cccagtgcag ctgctgggca atcagaccga
    22681 cagacatcag acgcgggtgt tcatggccct gcgcaacaat cagaatgttg ttttcgacgt
    22741 ctttgtccac cacataccac ggttcttcgg tgccttcttt ggtgccaccg atacccagac
    22801 ctttacgctg accgagggtg tgatacatca gcccctggtg ctcaccaatt tcatcgccgt
    22861 caacagtaat gattttgccc ggttgtgccg ggagataacg gcccaggaac tcgcggaatt
    22921 tacgttcgcc aatgaagcaa atgccggtag agtctttttt cttcgcggtg accagaccaa
    22981 gatcttcagc aatcttacgc acctgcggtt tttccagttc gccgaccggg aacaggcttt
    23041 gcgcaatctg ctcatggctg agcgtataaa ggaagtagct ctggtcttta ttgctgtcca
    23101 gaccacgcag caggcggctc ttgccatcga catcgacccg acgaacgtaa tgaccggtag
    23161 cgatataatc ggcacctaaa tcttcggcgg caaattcgag gaaggcttta aatttgatct
    23221 ctttgttgca gagaatatcc ggattcggcg tgcgaccggc tttatattcg gcaaggaaca
    23281 gttcgaagac gttgtcccag tactcggcag caaagttaac ggtgtgcagt tcaatgccga
    23341 gcttgtcgca gacagcctgg gcatcagcca gatccgctgc cgctgtgcaa tattcctcac
    23401 cgtcgtcttc ttcccagttc ttcataaaca ggccttcgac ctgatatccc tgttgttgca
    23461 acagccaggc agaaacggag gaatcaacac cgccggacat gccgacgatt actttttttg
    23521 cggtttcaga cattggatca ctcacgacat tgaacttcaa ggcggcgtat tctatcacgc
    23581 agccttttag atgacaccct ttgtaaaagg ccagttaaaa tcgccaatca tctccagcgg
    23641 atagcgttgc ccgctttgat aacaacgtat actttccgcc accagtggcg aacgaagatt
    23701 tgacgcctgt aaaatttctt cggcgctaac ccaacggcag cagtcgatat cgctgtcatg
    23761 aggctgggtc gggcatattt gctcaagctc aatggcaaaa aggaaacgca aaaacggcgt
    23821 tttatctggc gcaatccact gatgcatacg aataaagtgt tgcggctgcg cgctgatgcc
    23881 ggtttcttcc cacagctcac gggcggcggc ttccactaag gtttcatcgg cctccagatg
    23941 cccggcaggt tggttccata aggctttacc attaatcgtc tcttcaacga ctaaaaattt
    24001 gccttctgcg tgcaccacgc aagcaacggt aacgtgcggt ttaaacattt tgcatcctta
    24061 atctgtcact tctcgccatt cgccattagc aagattatcc aaagagtaat cacccatcgc
    24121 atagcgaatc agtcgcagcg tggggaaacc aacatgggcg gtcatgcggc gcacctggcg
    24181 attacgtcct tcatataagg tgatcttcag ccagctggtg ggaatacttt tgcgttcacg
    24241 aattggtgga ttccgcggcc ataaccacgc aggttcgtca accagctccg cgccggcggg
    24301 cagggtaggg ccatcattta aggttacgcc attgcgcaag gcttcaagtg cgtcttgtgt
    24361 gggaatgcct tccacctgca cataatagat ttttccggtg cgtttgcctg gctgggttaa
    24421 acgcgcctgt agtgcaccat tattggtcag caccaataac ccttcgctat cgcggtcaag
    24481 acgacctgct gcataaacgc cctgaaccgg gatgaattct tttaatgttt tgcgtccggc
    24541 ttcatcggtg aactgcggaa gaacatcgta gggtttattg aacaggatca cacgcgtggg
    24601 ctggttttca ggtttacgcc tggtagaacg ttgcgagctg aatcgcttaa cctggtgatt
    24661 tctaaaagaa gttttttgca tggtattttc agagattatg aattgccgca ttatagccta
    24721 ataacgcgca tctttcatga cggcaaacaa tagggtagta ttgacaagcc aattacaaat
    24781 cattaacaaa aaattgctct aaagcatccg tatcgcagga cgcaaacgca tatgcaacgt
    24841 ggtggcagac gagcaaacca gtagcgctcg aaggagaggt gaatggaaag taaagtagtt
    24901 gttccggcac aaggcaagaa gatcaccctg caaaacggca aactcaacgt tcctgaaaat
    24961 ccgattatcc cttacattga aggtgatgga atcggtgtag acgtaacccc agccatgctg
    25021 aaagtggtcg acgctgcagt cgagaaagcc tataaaggtg agcgtaaaat ctcctggatg
    25081 gaaatttaca ccggtgaaaa atccacacag gtttatggtc aggacgtctg gttgcctgct
    25141 gaaacccttg atctgattcg tgaatatcgc gttgctatta aaggcccgct gaccactccg
    25201 gttggtggcg gtattcgttc tctcaacgtt gccctgcgcc aggaactgga tctctacatc
    25261 tgcctgcgtc cggtacgtta ctatcagggc actccaagcc cggttaaaca ccctgaactg
    25321 accgatatgg ttatcttccg tgaaaactcg gaagacattt atgcgggtat cgaatggaaa
    25381 gctgactctg ccgacgccga gaaagtgatt aaattcctgc gtgaagagat gggcgtgaag
    25441 aaaattcgct tcccggaaca ttgcggtatc ggtattaagc cgtgttctga agaaggcacc
    25501 aaacgtctgg ttcgtgcagc gatcgaatac gcaattgcta acgatcgtga ctctgtgacc
    25561 ctggtgcaca aaggcaacat catgaagttc accgaaggcg cgtttaaaga ctggggctac
    25621 cagctggcgc gtgaagaatt tggcggtgaa ctgatcgacg gcggcccgtg gctgaaagtt
    25681 aaaaacccga ataccggcaa agagatcgtc attaaagacg tgattgctga tgcattcctg
    25741 caacagatcc tgctgcgtcc ggctgaatat gatgttatcg cctgtatgaa cctgaacggt
    25801 gactacattt ctgacgccct ggcagcgcag gttggcggta tcggtatcgc ccctggtgca
    25861 aacatcggtg acgaatgcgc cctgtttgaa gccacccacg gtactgcgcc gaaatatgcc
    25921 ggtcaggaca aagtaaaccc aggctctatt atactctctg ctgagatgat gttgcgccac
    25981 atgggttgga ccgaagccgc agacctgatt gttaaaggta tggaaggcgc aatcaatgcc
    26041 aagaccgtaa cctatgactt cgaacgtctg atggaaggcg ctaaactgct gaaatgttca
    26101 gagtttggtg atgcgatcat caagaacatg taatctctac atgtgttaaa tattgaaacg
    26161 ggcgtataac acgcccgttg ttttatttat gtggatatta ttaatagcat atcgagcata
    26221 tttat
//
LOCUS       DAZGBC010000061        26074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGBC010000061 DAZGBC010000000
VERSION     DAZGBC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.061"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(159..1046)
                     /gene="galF"
                     /locus_tag="ACHK1J_002418"
     CDS             complement(159..1046)
                     /gene="galF"
                     /locus_tag="ACHK1J_002418"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO7911669.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGIKFREKILKLL"
     gene            complement(1289..2284)
                     /locus_tag="ACHK1J_002419"
     CDS             complement(1289..2284)
                     /locus_tag="ACHK1J_002419"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO7911670.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKSPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(2442..3836)
                     /gene="wcaM"
                     /locus_tag="ACHK1J_002420"
     CDS             complement(2442..3836)
                     /gene="wcaM"
                     /locus_tag="ACHK1J_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO7911671.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(3847..5067)
                     /gene="wcaL"
                     /locus_tag="ACHK1J_002421"
     CDS             complement(3847..5067)
                     /gene="wcaL"
                     /locus_tag="ACHK1J_002421"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO7911672.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVDIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVVTPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5064..6344)
                     /gene="wcaK"
                     /locus_tag="ACHK1J_002422"
     CDS             complement(5064..6344)
                     /gene="wcaK"
                     /locus_tag="ACHK1J_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO7911673.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FHDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAHQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSCERQTGMQMVQSVLERIGEVK"
     gene            complement(6908..8386)
                     /gene="wzxC"
                     /locus_tag="ACHK1J_002423"
     CDS             complement(6908..8386)
                     /gene="wzxC"
                     /locus_tag="ACHK1J_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO7911674.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVVVFFLSDAI
                     GDVLNNPDLAPLIKTLSLAFVMIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTVGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(8388..9782)
                     /gene="wcaJ"
                     /locus_tag="ACHK1J_002424"
     CDS             complement(8388..9782)
                     /gene="wcaJ"
                     /locus_tag="ACHK1J_002424"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO7911675.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(9837..11207)
                     /gene="cpsG"
                     /locus_tag="ACHK1J_002425"
                     /gene_synonym="manB"
     CDS             complement(9837..11207)
                     /gene="cpsG"
                     /locus_tag="ACHK1J_002425"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO7911676.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTSLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(11400..12836)
                     /gene="cpsB"
                     /locus_tag="ACHK1J_002426"
     CDS             complement(11400..12836)
                     /gene="cpsB"
                     /locus_tag="ACHK1J_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO7911677.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGELTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(12839..14062)
                     /gene="wcaI"
                     /locus_tag="ACHK1J_002427"
     CDS             complement(12839..14062)
                     /gene="wcaI"
                     /locus_tag="ACHK1J_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO7911678.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQC
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAAERLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(14059..14538)
                     /gene="gmm"
                     /locus_tag="ACHK1J_002428"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(14059..14538)
                     /gene="gmm"
                     /locus_tag="ACHK1J_002428"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO7911679.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(14541..15506)
                     /gene="fcl"
                     /locus_tag="ACHK1J_002429"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(14541..15506)
                     /gene="fcl"
                     /locus_tag="ACHK1J_002429"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO7911680.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(15509..16630)
                     /gene="gmd"
                     /locus_tag="ACHK1J_002430"
     CDS             complement(15509..16630)
                     /gene="gmd"
                     /locus_tag="ACHK1J_002430"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO7911681.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(16657..17205)
                     /gene="wcaF"
                     /locus_tag="ACHK1J_002431"
     CDS             complement(16657..17205)
                     /gene="wcaF"
                     /locus_tag="ACHK1J_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO7911682.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(17221..17967)
                     /gene="wcaE"
                     /locus_tag="ACHK1J_002432"
     CDS             complement(17221..17967)
                     /gene="wcaE"
                     /locus_tag="ACHK1J_002432"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO7911683.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSS
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRRLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKS"
     gene            complement(18093..19313)
                     /gene="wcaD"
                     /locus_tag="ACHK1J_002433"
     CDS             complement(18093..19313)
                     /gene="wcaD"
                     /locus_tag="ACHK1J_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO7911684.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            complement(19288..20505)
                     /gene="wcaC"
                     /locus_tag="ACHK1J_002434"
     CDS             complement(19288..20505)
                     /gene="wcaC"
                     /locus_tag="ACHK1J_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO7911685.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(20502..20990)
                     /gene="wcaB"
                     /locus_tag="ACHK1J_002435"
     CDS             complement(20502..20990)
                     /gene="wcaB"
                     /locus_tag="ACHK1J_002435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO7911686.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNSVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(20993..21832)
                     /gene="wcaA"
                     /locus_tag="ACHK1J_002436"
     CDS             complement(20993..21832)
                     /gene="wcaA"
                     /locus_tag="ACHK1J_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO7911687.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(21925..24087)
                     /gene="wzc"
                     /locus_tag="ACHK1J_002437"
     CDS             complement(21925..24087)
                     /gene="wzc"
                     /locus_tag="ACHK1J_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO7911688.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAYRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(24090..24533)
                     /gene="wzb"
                     /locus_tag="ACHK1J_002438"
     CDS             complement(24090..24533)
                     /gene="wzb"
                     /locus_tag="ACHK1J_002438"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO7911689.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(24539..25678)
                     /gene="wza"
                     /locus_tag="ACHK1J_002439"
     CDS             complement(24539..25678)
                     /gene="wza"
                     /locus_tag="ACHK1J_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO7911690.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            complement(25878..>26074)
                     /locus_tag="ACHK1J_002440"
     CDS             complement(25878..>26074)
                     /locus_tag="ACHK1J_002440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911691.1"
                     /translation="NCRIVTLPAIPLAVTLPPALLESQYSITIMSPYIRKLISAITLS
                     HQQLYVERVLKIKLNQCIGS"
BASE COUNT         6111 a         7063 c         6494 g         6406 t
ORIGIN      
        1 atgtttcatt aaagctcatt tctctactct attaacctct gtaatatttc attaaaaata
       61 gaacgctacc gcccttggct cacagcaacc aaagcgctat ctataaacgt ttataaaaaa
      121 gtacgaggat atcaataccc ccgtagataa gatgaaaatt aaagtaactt taaaattttt
      181 tctctaaact taataccttc tttcaggttg cgcagcccat acttcacaaa cgcctgcata
      241 tagcccattt ttttaccgca gtcgtagctg tcgccggtca tcagcattgc atcaacggac
      301 tgttttttcg ccagctcggc aatggcatca gtcagctgaa tacgtcccca tgcaccaggc
      361 tgagtacgtt ctagttccgg ccaaatatcg gcagaaagca catagcgacc aacggccatg
      421 atgtctgagt ccagcgtctg cggctgatcc ggtttttcga tgaattcaac aatgcggctg
      481 actttacctt cgcggtccag cggctctttg gtctggatga cagagtattc agagaggtca
      541 cccggcatac gttttgccag cacctggcta cggcccgttt cattgaagcg cgcaatcatg
      601 gcagcaaggt tgtagcgcag cggatcagcg ctggcgtcgt cgatcacaac gtctggcagc
      661 accacgacaa acggattgtc accaatggcg ggtcgtgcac acaaaatgga gtgacctaat
      721 cccaaaggtt caccctgacg cacgttcata attgtcacgc ccggcgggca gatggactgt
      781 acttccgcca gcagctggcg cttcacacgc tgttcaagga gtgattctaa ttcataagaa
      841 gtgtcgaagt ggttttcgac cgcgttcttg gacgcatgcg ttaccaggag gatttctttg
      901 atccctgcag ccacaatctc gtcaacaatg tactgaatca taggcttgtc gacgatcggt
      961 agcatctctt taggaatcgc cttggtggca ggcaacatat gcatcccaag ccccgctacc
     1021 ggtataactg cttttaaatt cgtcattctt ttcctacctc taagtggctg atagtgcgtg
     1081 aattattgcc aaaggttagc caaacgttat ggctatatac caaagcttag ttccgcataa
     1141 ctttgattaa accttacgat aacactacac accatcagca tctgggttac tcggattact
     1201 cggaaatcaa catactgata atttaatcag tacctctttc cgaataatcg tagtccaacc
     1261 tggtcctttt ttctctgact cgtctgcatt actcagaaac aaacgttatg tcgtcttttt
     1321 tggcatgaac gaattcatac tgcagagttc gatccagacc ttgcgacagc gtatacggtg
     1381 ccacaaaacc tgaagaatgc actttcgttg cgtcaaactg tgttgttgcg cagaattttt
     1441 tcacgcgcac tgagctgaca gcgtattttt tgcccgtaat tttgctaagg atatcaaaac
     1501 aatatccacc cagcattcct agtgggtaag gcaagtgcat agaagggatc tttttgttca
     1561 ggctttgttc aacttcagca accaactggt tcatgttcag gtctggctta tcaacgtagt
     1621 tataaacctc ataacctgcg gcaacattct tcagtttgta cttgataaac tcaacaatgt
     1681 ttccaacata agccatggac ttatagttag tccctgcacc caccatcata aacttgccgc
     1741 cagcgatctg tttcagcaag ttatagacgt taccgcggtt gcgttcaccg aagataacgg
     1801 taggacggat gatggttaat gaacgttctg ttggtgattt gttataccat tcacgcagca
     1861 cttcctctgc ctgccactta cttttgccgt agtggttgaa agggtcgtgt ggatggtttt
     1921 cgtcagggtt gtgtttgttc aaaccataaa cagcaacgga actggtaaag atgatatttt
     1981 taacgccatt tttttccatg gcagccaaca cattgcgggt accctgaacg ttgacatcat
     2041 aatagagaga agtagggctg acgtcatcgc ggtgttccgc tgccagtagt acaacagtgt
     2101 caaaaccggc taacgcctgg tcgagtgcct gttgatcacg aacatcgcca atctgtgtga
     2161 tttctggata aaagtggctc tgctgtttgt ccaggttctt gatattaaag tcagcaattg
     2221 ccgtttcaag tagtcgggtt cctacgaatc cggaagctcc tatgagcaaa acgttatcgt
     2281 tcataaatca ctttagtctg gttgttacgt aagaaacaca agataaagat gagtaccttc
     2341 cctgagtagt caatgctgcc cagccccagc tttaacagtt agtgtgagga ttataatctt
     2401 ttagaacatt atatccagta agtttatgaa tggtcgcaaa tctactctct ccgttccggc
     2461 aatctaaagt taatcttttc cacattaaca atatggtgat taatcctgtc gatatcgacg
     2521 gagctttgcc ctctttcatt caccgcatga acatttgcaa gagacagcag tgtttctttt
     2581 ttcgccataa acacgccgcg aacgtctttg cgcatgtcga agttcatgct caatgcggga
     2641 ccaactgagg attcctgcat cactttgata ttacgcataa aaagatgttg cggtttattg
     2701 tgaagctcca gcgaggcacg cttcatctca atgttagtca gtgccacaaa ggaggcagca
     2761 ttcccggcgg agatttggat gccgcgcaat ttataagcaa gatgggtgtt atccagttga
     2821 atattattca ctcggaaatt ttgcggtatc gagagatatt tgcctttaat taccccatag
     2881 ccgattaaca tcccggcgct attaatcatt tcaatattat caatcacgaa attgtcacaa
     2941 ccgtaaatag cgactgtcgc gttatcaatg cccgctttct tactgaaatc cggcgtgata
     3001 ttgcgggctt tgatattacg gataacaaaa tgtttgccat tttcaacatg gatcaactgc
     3061 cgacaatccg atcccgtgat attcgccacg acaaagtttt tcactgcctg atcttccggg
     3121 taattattat cgtaagtgct tcccgcaagg cctatgccaa tgccccagtt gattttgccg
     3181 ttagtacagt tgatgcgctc gatgacatgg tcggagatca aaatatcact gtcgttaatt
     3241 gccacgttcc attcaatggc gtcgccctgt aagtcgctga acttacaatt ggtgatgttg
     3301 gcaccgataa tctggttatg aaatccctgg cgtaagatgg cgtaattagc gtggctaacg
     3361 gtcaggttat cgatggtcag gttgcgcatg acccgtttgt ttttgccgcc gatataaatc
     3421 tgcgttaccg gaccaaagcc gctcatcgcc agccctttga tggtgcagtc agaaccacgc
     3481 acatccaggg tgatgttatg catactgccg ccctcctccc ctgtcacctg gctgccgtcc
     3541 tgtaagacaa atcgccctct gccgttgccg cgcaggcttc caaggatgtg taacgttttg
     3601 ccaggaggaa tgaagatgcc ggtgttgata ttgtcacaaa cgaatccagc aggcacgacg
     3661 accgtttgcc cttcgctgaa ggcttgttta aatgaggcga tccagtcgtg tgggttgtag
     3721 tcgttaatgt taacgctttg tcgggcggga agtgcgcgag cgaaaggggt atggaggaag
     3781 gcaagcgccg agcttgccgt caggaaggtg cgtcgggaga gttttttaaa tggcatgcgt
     3841 tctcctctat aaagcttgca gcaagcttgc gagttctcga ttgatcacct gctggttaaa
     3901 atcatgttcg actttttcgc gcgcacgttt gacgaccgga gccagttcgt cggtgtccag
     3961 ttggctaaac gccgccagtc gttgcgccag tgcgcgagca tcgttctcag gcaccagcca
     4021 gccggatttg tcagcctcca ccagttccgg tattccgcta tgcagagtag aaaccaccgg
     4081 aatgccgacc gccatcgcct ccatcagcgc taccggaatg ccttccatat cgccatccgc
     4141 gcccgttacc gatggcaaca ggaagacatc cgcgtcgtcg agcatcgctt tcacttcgtg
     4201 gctcggttta aagcccggca tctctaccac atcttccagt tgatattgtt cgatgagggt
     4261 gcgcaggcgt cgttcccacg ggccgatgcc aaggatgcga tagcgaaatg ccacgccctg
     4321 ctctttcaac tgacggcagg cttcgatcgc cacatgcagg ccttttttct cggttaagcg
     4381 tgcgacggaa atgatttcca gcggtgttgc gggcgctttc accggacgcg ggctaaagcg
     4441 cgtcatgtcc acacccatac gcgatacggc gattttttcc ctcgggcagc ccattttttg
     4501 cagccttccg gcccacagat tacttatcgg taacatcagg tcgccacggc gaaacagttg
     4561 ttgatattcg ggggtgtagt ggttgagcac ttcccgactt gagatatcaa tgccgtggaa
     4621 gatagtagca attttgccgc gaatgacacc cagttcgcgg agttttgctg cggttacccc
     4681 tgctggacca aagtgagcga taaagacatc ggcatgaagc ggtgttacga cctggccgca
     4741 aatggcagac aaaatcaggt tccgcgactc ggcaccatag cgtttgaggt tgagcgcctg
     4801 ccaggtattt ttacgatgaa tgccgcgtaa cgtctggctg gcgcggtggc gcagtttcgc
     4861 cactttgccc gtaggttcgt cctgtaacca gcgggttctg gcggcaaggt tatatttcgt
     4921 ccatgccgcg tgggtgtttt gtgtgtcgcc tttttgcagc gcaacaatat ccacctcaaa
     4981 tcccatatca ataaacgcgg taatttgatt gaggacgaag gtttccgacg acagcggaaa
     5041 tttcaataag aagaagccga ccttcatttc acctccccga tgcgttccag tacagactgc
     5101 accatctgca ttcccgtctg acgttcgcaa ctgacggctt cgttaagtcg cgcgttcagc
     5161 gccggaagtt ggcctaaggt atccgcaacc atcgcttgca agctgccgtc cagtaaatga
     5221 cggatatcaa tcgccatctc tggcagcccc agttgttgca tgatcccggc ggatttatgt
     5281 tcatagttga tggcaatcgc cggagtagca aagttcattg agataatagc ggagtgcaga
     5341 cgcgtaccga cggtgagttc acaggcaccg agaattttgc ccatttccag atcgttgagt
     5401 tcatccatca ctacgtggta acgggcagga tcgctgatgt gctggcgcag gttgagcgcc
     5461 accatgcggt cgtctttgtt atagctgtcg atacccgtac aggtggagag cgcaatgacc
     5521 tgatagcctt cgtcgagaat gcgattgacc accccggcaa aggctttttc atacgcttgc
     5581 tgagtggtgc cgaggcgttt gtcgaacggt gccagttcgc gcagggtaat tgccaccgtt
     5641 ttctggtgtg cggcaacgtc cagccagtgt tggacggcat agctggcggt gaaatcttcg
     5701 gtgtggtgat cgaccagcca cgcagtatcg acgccatgtt ccacttttgc ggtagtgata
     5761 ttgctgcgtt tcattagatc gaggctgacc gattcgcgca ggatcagcgc gtcacagtga
     5821 ccgaaaacgt agttcgccag ttggttaaat tgctcatcat ggaacgggcc gacgctgtga
     5881 ccaatcataa acagcggctt tttcgccata aatgtgcaaa gcgcatgttc aaactgcggc
     5941 acgccgtaga gatcgacaaa aaacgatccg ccgacctgga taatggcgtc gtaacctgac
     6001 agcaggcgca cgaagtcggt gaatccctgg gcgatggcga tattacgcag cttgccagtg
     6061 tcggtgacgc gtgagagcaa tacctgatgc tggtagcggc gacggaggac ttttttaacg
     6121 cgcccgacaa cgcccgccgc gctgttgtgt tgtttcattt gcaggaacag cgggtcgccc
     6181 attaccgggc ggttgagcag ccaggaagaa ctgaccggat agcggctcat gacgtccact
     6241 tcggcgtgtg gattgaggat gttgatggca tcaagtaagc cgcgcaggat ggcgctgtcg
     6301 ccacgattgc cgcaagtgtg gttgcccaga ataagtaatt tcattttttc ctcataaatt
     6361 tgatgccagg tgaggttgtg tttgttgtgt tcgttgccgt tttttgcctg atgcgacgct
     6421 ggcgcgtctt atcaggccta caagacccga gcacagaacc gtaggccgga taaggcgttc
     6481 acgccgcatc cggcattcag tgcctgatgc gacgctggcg cgtcttatca ggcctacagg
     6541 tcccgagcac agaaccgtaa gacggataag gcgttcacgc cgcatccggc attcagtgcc
     6601 tgatgcgacg ctgacgcgtc ttatcaggcc tacaagatcc aagcaaagaa ccgtaggatg
     6661 gataaggcgt tcacgccgca tccggcattc agtgcctgat gcgacgctgg cgcgtcttat
     6721 caggcctaca agacccgagc acagaaccgt aggccggata aggcgttcac gccgcatccg
     6781 gcattcagtg cctgatgcga cgctgacgcg tcttatcagg cctacaggtc ccgagcacag
     6841 aaccgtagga tggataaggc gttttacgct gcacccggca accgttgtcg gagcctaaaa
     6901 caaccattca ccccgcccgt aaaagcattt tcattttttc gctgcgacaa aactgacgct
     6961 tcacttccac caccagcgga tggcgcgaca gcacaatcat caccacaaac gccagcacgc
     7021 ccgcggctat ttgcaccgcc agcagcatcc ccagcgccag ttgccctttc agcacaatgc
     7081 ccagcgcata actgaccacc agcgtcggca gagagagata aaacggcagc cacaaactca
     7141 ggatgtactg gcgataactg gaaccgagca ccggtttaat catcacgaag taactcagaa
     7201 tggtgttgat aatctgcacc agcaggaagc caaccgtgac gccgattgcg cccgccatct
     7261 gcccaccaat aacaatcgcc ggaataaaca gaaatgtttt gaatacgttg aatttaaagc
     7321 tgatatcgac ccgcgctttc gccatcagca gcgaaccaat cgggttaccc accgaacgca
     7381 gcagacccac cacacacagc aattgcagca ccggaataat gctgttccac ttctcaccaa
     7441 agaccagcgg tacaaagtta ttcgacacca ccatcagccc cagcagagcc ggaaagttga
     7501 taatccccac taccgacagc agcttgtaga agttaacgcg cagcttttcg gtgtcgtcct
     7561 gaattttggc gaatgccgga aacaacacgc gggtgatgat tgggttcagc ttcatcggtg
     7621 gcacaacggc cacgttgtac gccaggttgt atccccctgc cacgcccgcg ccgagaatac
     7681 gcgccagcac cagcgttgaa aggttggtat tgagatagtt gatgatgctg tccgccgtca
     7741 gccaggcacc aaagcgtaag ttcggtgcca ccgacgccag cgagaaatgc agaccggggc
     7801 gataaatttt gcggccaaag tagccaaaca gcagcgttct taccgcacta ttgaccagat
     7861 aaccgaggat cgcggtcatc gccagcggcc agaaatgggc gctaaccacc gtaaaagtga
     7921 agcccgccag tactgcgctg gtttcgatca tgccgatttt gttgaactcc agctccttct
     7981 gcatcagtgc gcggaactgc tgcccgtggg ggatcatcac aaacgccagc gacaatgttt
     8041 taatcaacgg tgccaggtcc gggttattca acacgtcacc aatggcatca ctcaaaaaaa
     8101 acaccaccac gcacaccacg atccccagcc cgacgttcag ccagtacaac gtggtgagtt
     8161 ccaggtgact gatttctttt cgctgaataa tcgagttagc gataccaaag tcagaaagcg
     8221 tatctgccag cgcgataatc accagcgaca cggtcagcaa accgaactgg tggttgtcga
     8281 taatccgcgc cagcacggtc atctgcacca gcccgaggcc gataatgatc accgtggcaa
     8341 tcgccgacca cttcgcgccg ctgatggttt tttcacgtaa gctcatatca atatgccgct
     8401 ttattaacga agcccttgaa taccgtcagg aaaacgattt tgatatcgaa ccagacgctc
     8461 cattcgcgaa tgtactcaag gtcgaattcg acgcgttttt ccattttctc cagcgtgtcg
     8521 gtttcgccgc gccagccatt aatctgcgcc cagccagtaa tgcccggttt caccttatgg
     8581 cgcagcatgt agccttcaat gagctggcga tattgttcgt tatgcgccac tgcgtgcgga
     8641 cgtggaccca caatcgacat ccccccggtc agcacattga taaactgcgg cagttcatcc
     8701 agcgaagtgc ggcgcagaaa gttccccact ttagtaacgc gcggatcgtt ctgcgtcgcc
     8761 tgagtcacca ctttgtcgtt ctccatcact ttcatggaac ggaacttcca cactttgatc
     8821 ggcttgccat ccatgccgta gcgagtctgg cggaaaataa ccggccccgg tgaactcagt
     8881 ttcaccgcca gcgcaataca gcacagcacc ggggaaatca gcagtaaaat cagcgttgcc
     8941 agcacaatgt cttccgcacg ttttagcagg cggttaaccc cggaaagcgg cgtgtcgtac
     9001 agcggcacca caggtacgcc gttcatctct tcgatacggg aatgaagaat attgaaggta
     9061 aaaacatcgg ggatcagcag caccgaacag gtggtatccg ccagttgatg gaccagtttt
     9121 ttcactcgcg cgccgtcgca catctgcatc gcgatataga cgttatgaat cttgcccgct
     9181 tttgcatcct cgaccagctg ttgcaggtta cccgcccagt cgttagaaac gccgcccggt
     9241 tttgggtcgt ggtatacacc caccacttca aaccctaacc acggctggtt acggaagctc
     9301 tccatcagca tttgcccggc ggctaaatcc cccgccaccg cgaccatgcg cttgttatag
     9361 ccatgattac gcagccagcc cgccccaatg cgaatacacg aacggcaaac caccagtccg
     9421 atgctggtca gcccatacca cgccagccag attttcagtt gcgtgtcgaa atcattgttg
     9481 aacgccacca gtccggcgct gaaaatcacg cttaaggtcc agttttgtag caacagggca
     9541 aattctgtcg ctgcccgaac accgcgccat gagcgataaa aatcggtgat gccacccagc
     9601 atctggaaca ccaccagcgt aatcagcgcc accaacaggt gcatgtagag gaatgacagt
     9661 ccgctgactt cgcaaaccag ccatagtccg gcaaacatga tggtgatatc tgaaaagcgt
     9721 tgcaccatag agattaacga tgcattggtt ttcgctcgct cgcgcttttt tagatttgtc
     9781 atcgttgttc ctgttattag ccccttaccc gcagtggggt aagggaagat ccgacattac
     9841 tcgttcagca acgtcagcag agttcgcgtt cgcgcttcca tcagcggcac atcaccgcgc
     9901 gattccacat tcaaccgcac caccggttcg gtgttggagg agcgcaggtt aaagcgccag
     9961 tcggcaaagg tcatgctgat gccatcggtg cgatccaccg ccagcgcctc acggctaaaa
    10021 tgctgttcga cgcggttaat cgcctcaacg gggtgcgcca gtttgctgtt gatctcaccg
    10081 cttgccggaa acgccgccat ccggtcgcgc accagttcgc ccagcgtttt tcctttcagg
    10141 cacaccagtt cggcgaccag cagccacggg atcatgccgc tgtcgcagta agcgaaatca
    10201 cggaagtaat ggtgggcgct catttcgcca ccgtagatgg cgtcttcctt gcgcatccgt
    10261 tctttaataa aggcgtgtcc ggttttcgac atcaccggcg tgccgccagc agcagtcacc
    10321 acatcaacag tgttccagga gagacgtgga tcgtggatga tcttcgcgcc gggatttttt
    10381 tcgaggaacg cttctgccag caggccgaca atgtagtagc cctcgataaa ctgccctttt
    10441 tcgtcaaaca ggaaacagcg gtcaaaatcg ccatcaaaag caatgcccat atccgcgccg
    10501 tgtttaatga ccgcattgcg ggtgtcgtcg cggcattccg gcagcagggg gttaggaata
    10561 ccgttgggga agttgccgtc cggtgtgttg tgtactttga ttaattccac gggcgcgccg
    10621 agggctttaa agcgggcttc aatggcgtcc accaccggac ccgccgcgcc gttcccggag
    10681 ttgatcacca gcttgagcga cgtgaggttt ttgacgttga tataaccgaa caggtgatca
    10741 acgtaagcgt cacgcaggtt gatttgctga tagcgaccgc gtttggtttc atcgacggga
    10801 ggaaagtcgt tggcttcagc caggcgctgg acatcacgca gtccggtatc accgctgatc
    10861 gggcgagccc cttcgcgcac cagtttcatg ccgttataat ccatcgggtt atggctggcg
    10921 gtaacttcga tgccgccatc cacgccgaga tggaacgtgg cgaaataaat ctcttcggtg
    10981 ccggacatgc caatatccag cacatcgacg cccgcatcct gtaaaccttt cgccagcgcc
    11041 agttttaagg tttcgctggt gaggcggaca tcgccgccta aaacaatggt tttcggttta
    11101 agaaattcgc cataggcgcg accaatacgc caggcaatat cttcattcag ttcttcgcct
    11161 aatttcccgc gaatatcata ggctttaaag caggttaatt ttttcatatc gttacccttt
    11221 ttcaggcaat agttggccct gaccgaaacg gccaattttg tttttgtcgt tatttacacc
    11281 gcggtttcgc attcattgcc tgatgcgacg ctgccgcgtc ttatcaggcc tacaaaaccg
    11341 aaccgtaggt tggataaggc gttcacgccg catccgacaa acagcgcctg atgcgacgtt
    11401 tacacccgtc cgtagcgatc cgcgaagcgc accacatcat cctcttcgag ataagagccg
    11461 gagcgcactt caattaaatc gagcggaatt ttccccgggt tttccaggca gtgcgtcgcc
    11521 cccagcggaa tataaatcga ctcgttttca ccaagcagtt tgatatcacc gtcaatagtg
    11581 acttttgccg ttcccgcgac aaccacccag tgttccgcgc ggtgatggtg catctgtacc
    11641 gacaagcctt cgcccggttt cacggtgatg cgtttcacct ggtagcggtc gcccgcgtcg
    11701 atagagtcat atttgcccca cgggcgatag acttcgcgat gtacccgatg ctcatggcga
    11761 ccatcggctt tgatctgctc gaccactttt ttcacatcct gcaccgcgtt acggtcggca
    11821 atcagcaccg catctttggt ctgcaccact accagatctt tcaccccgac ggtggttacg
    11881 aggccagatt cggcatacac atagctgttt tcagttttgt gattaatcac atcgccgtgg
    11941 caaacgttgc cctcggcggt gtgggcgctg atctcccata atgaagacca ggaaccgaca
    12001 tcgctccagc ccgcatccat cggcaccaca acggcatctg ccgtacgttc catgaccgcg
    12061 taatccaccg actcttccgg gcaggccaga aacgcttctt catccacgcg aataaaatcg
    12121 agatccggat cgacggcact cattgctttt tcacaggcat cgagaatatc cgggcgatat
    12181 tttttcagtt cttcgagata gcgtccggcg cggaacagga acatgccgct gttccagtaa
    12241 tattcgccac ttgccacata ggcctgggcg gtttccagat tcggtttttc gacaaactgc
    12301 gccacttcaa aggccaccgt atcctgctcg cccgcagata cttcaccgcg acgaatatag
    12361 ccatatccgg tttcaggtag atccggcaca atgccgaagg tcaccagttt gcccgcttcg
    12421 gcatatggca tggcattacg cacggcggca cggaacgcgt cttcatcggc aatcacatga
    12481 tccgccgcca gcaccagcat taacgggtcg ctctccggac tatgacgtgt tgccgccagc
    12541 gccgccagcg caatggctgg tgcagtgtta cgccctgctg gttcgagaat aatgttctcg
    12601 gtgagtttgt tcagttggcg cagctgttcc gcgacaataa agcggtgctg ctcattgcaa
    12661 atcaccaccg ggctttcgca ctccacgccg ttcaggcggc agatggtggt ttgcagcatg
    12721 gtgagttcgc ctttcaggca taaaaactgc ttgggataaa gtacgcggga aagcggccat
    12781 aagcggctac cggagccacc tgccatcaca actggataga gtttcgactg cgccataatt
    12841 atccccgaat atcatttata aattgacgta acacgttctc tttatcgagc gtgcgttcgg
    12901 catattcacg tgccaccgtg ttgtgtttgg gcagcaggag cgcctgacgt atccccgcca
    12961 ccagcgcctc gaccgattcc ggttctacgc aaaccgcaat gcccggaaag gtttcgcaaa
    13021 gctgtcccag ttctgtgtgg gcctctgcgg taatcaccgc gttaccgcct accgccagaa
    13081 tattggtcag tttcgacggc aatacggcat ctgccgcgcc gcgtttttgc accaccagat
    13141 ggcaatcgcc catcttcagc agtgcgggta aagcgtcata cgattgcagc gggaagaatt
    13201 gcatgttgtg cagtccacgc tgctgcgcca ttttttccag ccgcgctttg ccgccgccct
    13261 gcccgacaat ggcaaaaatc agcggttcat cacgcaggcg ttcggcagct tcaataacgt
    13321 tttccagccc ctgcttttca ccaatattgc cggagtaaag aatgattttt ttgttatccg
    13381 gcaggccaag ctggttacga agggcatcaa catcggcgtc tgcaacatgc tgaaaacggg
    13441 cgatttccga ccagttgggg aagaaaatga cgttatccgc cgccacgcct ttttcgatgg
    13501 ctttattcat catcgaacgc gaaatcgtgg agacgttatc gacgttatgc agtccgctac
    13561 gttcgaacgc cgttgccagc tgtgccactt tgccgccttt gccttttccg gcaaggccca
    13621 gccccagcat ggcgtccact tcgtaatcct gaatatgcag cacggtacgc gcaccagaga
    13681 gtttcgccag caggcgcatt cccggcgtgc aaaacagcgt tggcactacg ccgataatgc
    13741 gatccggctt ccagcgacat tgcgccatca acgggaaaaa actgctgacg gcaaaactgc
    13801 cgagatgtaa caggcgtttc agggtgctcg gctgttttgg cacgtacagc gggcagcgcc
    13861 acaccgtagc ggccccctct tctcgtttgt agcgccaggc ggaatagttc tcgcccacct
    13921 gccactgcgg gtagtaaggc ggtgcggtaa tgacccgcac ctcatgacct tgtgccgcca
    13981 gccattccac catctcgccg gtgtatttgc cgatgccggt taactccggc gagtagttaa
    14041 tgccgtagac cagtattttc ataatccggg tactccggca cgcttctcag cgagaaaata
    14101 ggcgcggctg ttagcgtgaa cattgtcact ggcgagcagc gcgtccggcg tcaaccagcg
    14161 gtaatcgtca tgctgctcat ccggcagtaa cagttcttct tccgctactc tgaagcgaaa
    14221 accgagcacc acatagtgag tggtgaaatc cgtgccagag aagttatcgt cataaaagtg
    14281 ctgccagaca ccgtaaaact ggcctgctgt tatcggcaga cgcagcccca gttccgccat
    14341 cgtcagccgc tcaaatgcgg cttccagcgt ttcgtctttc tgcacgcgcc ctcccggcac
    14401 aaaccagtaa ccctgcgccg ggcggttggt tcttttgcca agcagaaact cgccgcgact
    14461 gttctcgaca ataaagtcga gagagacaag cggagtggag cgcactaccg tggcaaagtc
    14521 ttcctgacgt aaaaacatca ttacccccga aagcggtctt gattctcaag gaaccactgg
    14581 taagtgctgg caagccccgc ttccagtgag atttcgtgat accagccaag ctgatgcagg
    14641 cgcgtcacat ccagcagttt gcgcggcgta ccatccggtt tactggcatc aaaaaccacc
    14701 cgacctttgt aacccaccac tttggcgatg gtttgcgcca gctcgcggat agtgcagtca
    14761 acgcccgtgc cgacgttaat gtgcgacagc atcggctggg tgttctccag ccagacttcg
    14821 tgcgccagct ccatgacatg aatgctcgcc gccgccatat catcgacgtg caggaattca
    14881 cgcatcggtg taccgctgcc ccacaccacc acgtccggtg cattctgtgc cgtcgcctcg
    14941 tggaagcgac gcagcaatgc agggatcaca tgcgaattac tcgggtggaa gttgtcatgc
    15001 gggccataca ggttggtcgg catgaccgag cggtaatcgc gtccatactg gcggttgtaa
    15061 gattcgcaca gtttgatccc ggcgattttg gcaatggcgt aaggctcgtt ggtcggctcc
    15121 agcgtgccct gcaataactc gctttctgcc atcggctgtt ttgccagttt cgggtagatg
    15181 caggacgatc cgagaaacag cagtttgttc acgtcgttct gatgcgcggc gtgaatgatg
    15241 ttgctctcaa tcatcatgtt ctgatagatg aaatccgccg gatacgtgtt gttggcaaca
    15301 atgccgccca ctttcgcggc cgccagatag acctggtcaa tacgttcgct ggcaaagaaa
    15361 tcatgcacgg cgcggctgtc cagcaggttc agctcgtcgc gggtgcgtaa taccagttcc
    15421 acatcaccgc gctgttcgag ctgccgcctg atggcggaac cgaccatccc gcgatggcca
    15481 gcaataaaaa tgcgttgtct actcatactt atgactccag cgcgatcgcc acgtcgtagc
    15541 cgtgagattt cagcagagag tgttttttcg ccgcttcgag gtcattagcc accatttcag
    15601 acaccatctc tctgagggtg atttccggtt tccagcccag tttttcgtgc gctttggtcg
    15661 ggtcgccgag cagcgtttca acttcagccg gacggaagta acgcggatca acggcgataa
    15721 tcacatcacc cggtttaacg cccggcgcgt catgtccggt gacggaaacc acaatgccct
    15781 tctcttcaac gcccgtgcct tcaaagcgca gtttgatgcc cagctgtgcc gccgccattt
    15841 ccacgaactg acgtacggag tactgaacgc cggtagcgat aacgaaatct tccggttgtt
    15901 cctgttgcag catcatccac tgcattttta cgtagtcttt ggcatggccc cagtcacgca
    15961 gggaatccat attgccgagg tacaggcacg attccagccc ctgggcgatg ttggcgattg
    16021 cgcgggtgat tttgcgggta acgaaggttt cgccgcggcg cggggattca tggttgaaga
    16081 gaataccgtt gcaggcgtac atgccgtagg attcgcggta gttaacggtg atccagtagg
    16141 cgtacagttt ggcgaccgca tacggagatc gcgggtagaa cggcgtggtt tctttctgcg
    16201 gaatttcctg caccagacca tacagttcag aggtggaagc ctgatagaaa cgggttttct
    16261 tttccagacc gaggaagcgg atcgcctcga gcaggcgcag cgtacccatc gcgtcgacgt
    16321 cagcggtata ttccggtgac tcaaaagaga ccgcaacgtg gctcattgcc cccaggttgt
    16381 acacttcatc cggctgtact tcacgcaaaa tgcgcgtcag gttggaggta tcactcaggt
    16441 cgccataatg cagatggaat ttcgggttgc aggtgtgcgg atcctgataa atgtgatcca
    16501 cgcgctcggt gttgaatgac gatgcgcgac gcttaatacc atgcacctcg taaccttttt
    16561 ccagcagaaa ctctgccagg taagaaccgt cttgtccggt tacaccggtg atgagagcga
    16621 cttttgacat gtcttattcc tctgtatttt ttgaatttat tcagtttcaa cgcgttcgcg
    16681 tatcaccact gcgggattcc cccggcaaac cacatttgcc ggaagcgatt taaaaacact
    16741 gcttcgtgca cccacgacgg tgccgtcgcc aatcgtgacg ccaggggcaa caaaaacatc
    16801 tgtcgccagc cagcatttct cgccaatcac aataggcgtg gcgttaatgg tgaaatgttg
    16861 acttgcatgg tcgtggctac cggtgcataa ataacttttt tgcgatatca ccgaatgtgc
    16921 gccaatggtt atttcaccga gggtatataa attgacgtca tcgccgaccc acgcgtaatc
    16981 acctaaggtt aatttccacg gataggtaat ttttactgac ggacgaataa ctacgttttt
    17041 tcctattttt gcgccgaata aacgtaataa aaaagcccgc cagcgataca atacttgtgg
    17101 cgaccaggca aatattgttg cctgtactgc ccaccataat tgcactttta tagcgttgcc
    17161 gccccggaac cctttcggca ccgagaatcc acttaaatct tgcatcgttt tcccttatat
    17221 tcagcttttg ttatataagg ctttagtctt acctgtcgta cgctggcgta aatgccagga
    17281 taattcagcc cagaagccag gcacatgtaa tatttgacgt tggacttttt tggcgtcagc
    17341 acacaattcc atattattgg tggtagatac cccacccata gaaaattcag acaccagtcc
    17401 attgagtttt ttaaatgcat aaccggcttt atacattttg gctgccagcg cgtaatcaga
    17461 agaaacttta tattccaggt cataacgcca ttttttcaag ccggataccg ggaaaaatat
    17521 cgcctgatga ctggcgggca gactgtgata aatataccaa cccggtttgg cgctacgttt
    17581 aattttatgc ccgtcgccaa aatccagtag cgcatcgccg gtgatcatca cgttatcttt
    17641 ttgcactttt aacctacgga caaaatttgc ggcatcctga tgaaaaatat cacctgagtt
    17701 gagaaacaac gcgaacttac cttgcgccat cgcaatgcct ttgttcatgg cgtcgtagat
    17761 accgttatct ggctcgctga caaagcgtag gttatagata ccattgagat tttccaggta
    17821 ctcacgggtg ccgtcactgg aaccgccatc gacaacaatc cattcgaagc tgatatcgtc
    17881 cgcctgcgcc agatgcgcca gcgaggcgtg tgttttgact atcccttcga ggttacgaaa
    17941 cgcgacagtg attatgctaa gcaacatgtt ttacctcgta atacccgtca tacttcaagc
    18001 ggcatgtgcg ttggttttcc tcgctcaccc cagtcacgta ctcgtgtacg cttctgggga
    18061 ttcgctgcgt caccaacttc ctgcaactcg gattatttag ggtgtatatt taacgctttt
    18121 cgcaaaataa acggacagac gattaaaaat gcatattccg ggctaaatat cgaaccggta
    18181 aaaaacagcg ataccggagt aaaaagatat aattgcacgc gaaaattgcg gttatcacca
    18241 aaagcgttga tcatcatttt tatcactttc cccatgtacc acagcgataa aaacaccgcg
    18301 aaccaggaaa aataaataat cagcagatac aaaccattgt ctatggtttt tccgacatcc
    18361 gcaccgttaa atattccgaa tgatgcgaca tattcataaa gtgagccaaa tctgactaca
    18421 ccgtcaatat gggtcaaaga ataaccgacc atcaccagcg gaccgacaat acgataatac
    18481 gatgacgatc cttccgtacc taaatcgccc agacgggtgg aaatataagg aaacgcgatt
    18541 accacaccaa ccaggaatac agccagagaa atcaatgcta acggtaactt tttcttaatc
    18601 gcctctttat tcagatattg aaatgcccac tccagcaaat aaaacaggat aaaggtcata
    18661 acccctgaaa acgatccgga taatattatc cctgcgagaa tcatagcatc ggttttaggc
    18721 gttttgatac caaactgttt gatgctgagc caaattgaga ttaatgccag agcgaaaaat
    18781 gccggttcga aataaagtgc tgtcgtgcgc ttgccgccga atttaatgaa attcagcaca
    18841 tagctgttgc tgtaaatcag atatttcgaa atcgactcca taatactgct gccaccggtg
    18901 aggataattt gcgccatctc caccgccgcc agcgccacca ccagcccgac caccagataa
    18961 aagaaacgta atatcttgcg atggttgtgt ggcgaaatcg ttttaaagcg aatactccac
    19021 accatgccaa taatgatcac aatatagaca aacagcatgg ttgaagtgac gtatttactg
    19081 gcatccaacg actgaccaaa caggtagtta aacgccgtga gtcccgcgcc aatccctaag
    19141 gcaatcatca attttttaac gctgatgcgt tctaaaaaca gcagtaacaa gacaggtaaa
    19201 aaagtgacga tggtgatggg gaaactttcg ccaagctgag cgattttgac gttaaccagc
    19261 aggtagatca gcggcagcag caggtagcta cagattctga tagaagttga catactcctc
    19321 cagcatctgt tgtccactgt aggcggcgcg gctgcgttgg ctgaactcag ccagcgtggt
    19381 accaaatatc gcctgcgcga tttccggttt gcttaactgg accagttgca gcacctcttc
    19441 ttcgctgacg gttttaccgc cggatttttg caacacttca cgcgccgcgt cgctatgggt
    19501 ggcaatcacc ggcacgccaa ttgataacgc ctcacacaaa atcagcgggt agttatcgac
    19561 gcgagaactg aataccagcg catccatctg attgagcgcg ctcatcaact tgcgcttgtc
    19621 agtttcaaag ccgtgattaa ccacgttgcc agcggtgaac ggcgagaact taccaaaggt
    19681 atgcagttcg attttgtcgc ccagcgccat catctcgcgt accagttgct ggttagtttt
    19741 gccgtcgtaa cgcaggtcat gcgctaccac cgcgattttc ggcttaccct gagtttcgcg
    19801 caccggaggc aaatccgcca gaatcgcctc ggttgccata tcaataccgt tattgataat
    19861 ccggcaacgc cctggaccgt acaggctatt gaaagcgtca gccacatgct ggctggggga
    19921 aataaactga cagcccagcg ccagcatctc acggaataac tggcgtttgc ccgccaccag
    19981 ctggtgcgcc cgatcaatct tcaccggcgg atagttattt aaggtcgggc atttctggca
    20041 gcccgttttc cagccttcgc aaccgtcggt aaaggcgcag cgtccggtta cactccagtg
    20101 gtcgtgcagc gtccagacca gagtgacgtc cggtttgtgg tttttcacct tttcgcaaaa
    20161 gcgcaccacg ctcttaagat ttagccagta gctgtgcagc acatgaaaat gaaggaccac
    20221 cggacccggt gtgcgagtaa tggtgcgata taactcattg aaattgccaa acagatcgcg
    20281 attaaacaga cgaaacaggg caatattcgc cattgcggtc atccgcggcg tatgtttgat
    20341 tacctgcgga tagttctgat ggctgacgct ctctttgccg cctttgccgt agccgtagac
    20401 aaaatgtgac gccagcccct gttgcagcgc gcgctggtgg agatctaacg ccacacctgc
    20461 tgccccgcct tccgccagtc gcacattaaa ttgcagaata ttcatttaat taccttcact
    20521 cgcgcttttt ctcccaccac cagcgcgttg tccgggacag agtcgagcac cacgctgccc
    20581 gcgcccacgg tgacgttgtt accaagcgtg atatcaccaa gaataatgac gttggcaccg
    20641 agttcgacgc tgttgccaat gtgtggacat gccatgttat cggcaccacg attgccgata
    20701 gtgacgccgt ggcgaatggt gaaatcatcc cccgccacca cgtttttatt gatcactacg
    20761 gcgtaaccgt gatggatagt aaagcggcgg ccaatggtcg cggcggcctg gatttcataa
    20821 ccgaaaaagc attcggtgat aatgcgatac agcaccagca gcggggccgc ccacagattg
    20881 ttgaggacgt tctttttgcg ccacaccgaa caaaaatgag cgacgcgata ggcaagaacc
    20941 atgcagcacg ggcgtaaact ccagctgttg gcgcgtagat cttccagcat atttagcgcc
    21001 cccgaatacc atcagccaga cgtttgccgt tacgcaccga cagcagcgtc agcaaagtgc
    21061 gccaggtcat acgtttattg cggatctggt agagagtaaa cagctgatat tttttactgg
    21121 cacggtcgaa tttgtctttg tgcttgcggt aaaagtggaa gtatccggag aattttttcg
    21181 gtgacgaggt gatctgcatt tcaccgtgat tgatatgcag gatctgcgtc gcttcttcta
    21241 ctttccacgg ttcgccatac tccaccacca tccgcaggaa gatgtcgtaa tcctgtgccg
    21301 ctttcagttc ggtatcgaac aaacactctt tgaaacgcca cgcccaggta aagacctgat
    21361 tgccaatgat attgcgttta tagaacaggc ggcgtgagta cggcgatttg ggatacagtg
    21421 gtaagcttgc cggttgggaa taaacttcgc cctggcaaac gtaatcgtta gcgtacaaaa
    21481 aggcgtgagt gaccagttgc tgtttatggg cgaggaagac gctcagacgg ttgggtgtcc
    21541 attcgtcatc gtcatcgatc ccggtaatgt attccccttg cgccagcata atagcctggt
    21601 tacgtaccgc gcacgccccg ctgttaatgt cgttgtgaat gtaggtaata cgcggatcgt
    21661 tgagggcggt gacgtactgt tgcagttgtt cccaagaggt ggagcaatca tccacgatga
    21721 tcatctccca gttgctgtag tcctggcgca agaccgattt tatcgcccga atcgccagtt
    21781 gttggcggtt ccaggtcggc atatagattg agatcagcgg attgtctttc ataggttgtt
    21841 ctcccggctt atgagctggt ttgcttgccg gatgcggcgt gagcgcctta tccggcctac
    21901 ggggcggtgc gaatgcaggc gtcgttattt cgcatccgac ttatattcgt attcgtaata
    21961 gccataatcc tgatacgcgc tggcgcggcg gaagatggag ttcagaatca cccctttcac
    22021 cggaataccg ttttgctcaa agcggctcag actggtttcc acttctttca atgtgttgac
    22081 cgcataacgc gccaccatta acgtggttcc aacatgacga ccaacaattg ccgcatcggt
    22141 cactgccaga atcggcggcg tatcaatcaa caccaggtca tagtttttac tcgcccagtt
    22201 caccagttcg gcaaagcgtt cgctcatcaa cagttcagaa ggatttggcg gcacctgacc
    22261 gcgcgggatc aggtcaaatt tggcaataga ggtcggttta gctgcggtag taatatcgcc
    22321 ctgaccaatc agaatttccg acaggccatt gacgttattg gtgcccaaca gctcgtgggt
    22381 gtagcctttg cgcatatcgc agtcgatcaa caacacgcgt ttattggtct ggctgatcac
    22441 cgccgccagg ttggcgcaga caaaggtttt accgattgac gggctaaccc cggtcatcat
    22501 caacacattg ttctgcgcct gcatcatcgc gaagtgcaga ctggtacgca gactgcggat
    22561 ggcttcaatc gccagatcgg ttggattccc caccgccagt agctggctct gtttatagcg
    22621 tttaactcct ttgatggttt tgacgctatc gcgcgctttc tgccactccg acagcgggat
    22681 gctggcatag acgctgatac cgtgttcttc cagcacctgc gggctttcga tgccgcgatt
    22741 aaacaacgag cgcagcagca cacccacgat agagagcatc aggccgagga taatcgcccc
    22801 aaggataatc agccctttct tcggttttag cacgccaggc tgggtgattg ccgggtcaac
    22861 aatgcgcaca tcaccgacgg tgctggcctc ggtgattttc agctcctgct ctttattcag
    22921 cagttgcata tagacctgct gaccagactc gacatcgcgg gtcagacgga caatctcctg
    22981 ctgggttttc ggcatcgccg ttacgcgacc gttaagtttg gctttttcgt cttccagcgc
    23041 ctgacgtttc tccagcagcg tgcggtacgc ggggtgaact ttggtgtaca gcttggagat
    23101 ttccgcctct ttaaaggtca gttcgttcaa ctgcgcgtcg atgttcacca tcgaatcgag
    23161 caccgctttc gcttccagcg gcagatcaac agaatcttta tcctgacggt aggcattcag
    23221 tttgttttcg gcaacatcaa ggcggctacg tacttccggt aactgttgcg cgaggaaagc
    23281 gaggcttttc gacgcttccg ccgatttgcg ctcaatattt tgttcctgat agttacgggc
    23341 gatgctgtta agaatgtcgc ggatctgttc gcgatcttca ccggtataag tcaggctcag
    23401 tacgcctgcg tctttgccgt tctccgttac cgtcaggctg ttttgcagtt ggttgatcat
    23461 ccccagcgtg gagtatttgg tgacggtaaa ctcactgccc gggctggcgt gaatagcttc
    23521 aaccatcagc gtgacgcctt cttttttcag catctggccc gcttgtccac gggcgctaaa
    23581 gccgccatca ctgctcaggg tgtagttttt gtcatccagc acattaagcg taaacacctg
    23641 atccgccatc tctttcggac ggttaaaggt agtcactttc accgtctcgt tctgacgtcc
    23701 catcaggcga tcccagcccg caccgaaaat cgggaacgtg tttttgctca ccgcaatatc
    23761 gaggtcgaga tcgtccaccg ttttaccaag caccaggcgc gaacgaatca actggatctc
    23821 ggcgtccgat gcaggcggtt tgttggctaa cgccgatccg atatcctgca ctaacgaatt
    23881 gccgctgctt tgctcgattt gtaccagtgc gtcggcacta taaatcggcg tggcgaagaa
    23941 ggtgtaaacc acggcacaaa gggcgaatac agcggtgatg ccaatcaccc accagcgcgc
    24001 ttcaatgacg gtgccgacca ggcgaccaat atcgatttca tcactgcccg ttaccggagc
    24061 ggcatgttgt tttacttttt ctgtcattct tatacctgct ctgcgttcaa tgcctgcgcc
    24121 cactggcggg cagaccgttc aagtaatgtg tacaccgctg caaacgtttc ccggcttttg
    24181 cgatacggat cggggatttc acattcgtta tcccagtgac caaacagcat cactttgccg
    24241 cgcatctccg gtgccatctc gcataagcgt tcgatatggc gcttttccat ggtcaaaatc
    24301 aggtcatagt tgcgacacag gcggcggctg atttgtcgtg cacagtggcc ttccagagac
    24361 agttgatgtt ctgcggcgac gctgatagcg gtaggatcag cgcccttacc gaccagcgcg
    24421 ccgagtccag cggactccac ttttagctcc gggtgataac gttgcagtaa gcgttccgcc
    24481 gtcggggaac ggcaaatatt gccgacacag acaactaaga tgttgttaaa catgacgatt
    24541 accagttatg aatgtcgctg gctgtatccg tcatgtaacg gacaccgcta atagttggca
    24601 gcaactgatt gatcagacgg ttccagcggg aaaccggggc ggtggtgaca tacaccacgt
    24661 cataaggttg caggcggaat tctgtcgcca tcaccagcga cgtggcatcg gacatatcca
    24721 gctggtagat attggcaatc ttgccgttac gcccgccctc gcctttcagc ggacgaatga
    24781 caaagatgcc gctggcgttg gaggtggtca tgtcaatgcc ttcagcattg cccagagctt
    24841 cagtcagggt catgccgcta aagtccattt tcagggtgct ctgtttcttc acttcaccca
    24901 tcacaaatac tttcagatca tcattacgcg gcacgtagag aatatcgccg gggtaaagca
    24961 ggcgattctg gttgaggtcg ccgttttgca tcagcgcctg caaagaaatg cgctcttcac
    25021 gaccattgtg tgttagcacc acgttgcgcc agtcagcggt gtcggtcagg ccacctgcgg
    25081 cgttgatggc gtcgagaatg gtcagtggta cgttggtgat cgcctgttga ccagatttat
    25141 tcacctgacc tgatatatag gccttttgtg agcggaaggc ggcgatatta acgtccacct
    25201 gcgggtcagc gatgtacgtc gctaagcgcc cggtaatatc actgcggatt tcagcgagcg
    25261 ttttcccgac tacgtgaacc ttgccgatat acgggtaaaa catagtgccg tcaggctgta
    25321 cccagttgcc ggtgtcgctg gagctgcggt actgaccggc tggcgtggtg agttccgggt
    25381 gatcccagac ggtgacattc agaacgtcgc ccggcccgac gcgatactgg taattcgcga
    25441 tctcactttc cagcgtcata ttggggcgcg ctacgttggg tcgtggacgt aattgatcaa
    25501 tcaggcgcgg ggtcaacgga taaacattca ccattttgtc gagatcgaaa tcagcgtcct
    25561 gctgtttgat gacgtctttg cccatcgtcg acatattgct gcccggaagt actgtgcaac
    25621 cgcttatcaa ggttactgac accaataatg gcatcaattt cattttggat ttcatcattg
    25681 tttatttatc actttggcag agtaattatc ctgtgcacta ttaatagcaa tgtcgccatg
    25741 cacatttacc ttgcagttaa ttgaataaaa atttaactgg catcagtcct aaaaaaattg
    25801 atttcatccg caggctattg acagaataat tcagactggt ctttcaggca tccagacacg
    25861 ctaccgcccc tggcttttta gctaccaata cactgattta gcttaatttt taaaaccctc
    25921 tcaacataca gttgttgatg agaaagggtt attgcggaaa ttaacttccg aatataaggt
    25981 gacattatgg taattgaata ttggctctcc aataatgcag gaggaagtgt tacagctaac
    26041 ggaatagcag gcaaggtaac aattcggcaa ttgg
//
LOCUS       DAZGBC010000062        26057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGBC010000062 DAZGBC010000000
VERSION     DAZGBC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.062"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHK1J_002441"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHK1J_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO7911692.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHK1J_002442"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHK1J_002442"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO7911693.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(938..1405)
                     /locus_tag="ACHK1J_002443"
     CDS             complement(938..1405)
                     /locus_tag="ACHK1J_002443"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO7911694.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHK1J_002444"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHK1J_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO7911695.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHK1J_002445"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHK1J_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO7911696.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHK1J_002446"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHK1J_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO7911697.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHK1J_002447"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHK1J_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO7911698.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHK1J_002448"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHK1J_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO7911699.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHK1J_002449"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHK1J_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO7911700.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHK1J_002450"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHK1J_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO7911701.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHK1J_002451"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHK1J_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO7911702.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHK1J_002452"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHK1J_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO7911703.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHK1J_002453"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHK1J_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO7911704.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHK1J_002454"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHK1J_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO7911705.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHK1J_002455"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHK1J_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO7911706.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHK1J_002456"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHK1J_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO7911707.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHK1J_002457"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHK1J_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO7911708.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHK1J_002458"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHK1J_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO7911709.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHK1J_002459"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHK1J_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO7911710.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHK1J_002460"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHK1J_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO7911711.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHK1J_002461"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHK1J_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO7911712.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHK1J_002462"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHK1J_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO7911713.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHK1J_002463"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHK1J_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO7911714.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHK1J_002464"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHK1J_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO7911715.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHK1J_002465"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHK1J_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO7911716.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHK1J_002466"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHK1J_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO7911717.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHK1J_002467"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHK1J_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO7911718.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHK1J_002468"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHK1J_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO7911719.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHK1J_002469"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHK1J_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO7911720.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHK1J_002470"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHK1J_002470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO7911721.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKGEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHK1J_002471"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHK1J_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO7911722.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHK1J_002472"
     CDS             15545..15913
                     /locus_tag="ACHK1J_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO7911723.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHK1J_002473"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHK1J_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO7911724.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHK1J_002474"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHK1J_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO7911725.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHK1J_002475"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHK1J_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO7911726.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHK1J_002476"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHK1J_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO7911727.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHK1J_002477"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHK1J_002477"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO7911728.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDNWLALLDEAGMKGFPHTDYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHK1J_002478"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHK1J_002478"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO7911729.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHK1J_002479"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHK1J_002479"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO7911730.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHK1J_002480"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHK1J_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO7911731.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHK1J_002481"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHK1J_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO7911732.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHK1J_002482"
     CDS             23092..23634
                     /locus_tag="ACHK1J_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO7911733.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPICGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHK1J_002483"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHK1J_002483"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO7911734.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVAALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHK1J_002484"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHK1J_002484"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO7911735.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQTLGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHK1J_002485"
     CDS             25031..25288
                     /locus_tag="ACHK1J_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO7911736.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHK1J_002486"
     CDS             complement(25264..25818)
                     /locus_tag="ACHK1J_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO7911737.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6683 a         6165 c         6764 g         6445 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccacaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaatgattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgct acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag gtgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta caacgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaatgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgtcatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcag cggagggaag caattcagct tctggatatc ctgagaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tggacaaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctagagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact gacaggatta cactcgcgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgagt agagaaaaat
    16501 aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggcc ttctgctttc aggcgtttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccttgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagag gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgtaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tctagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcttcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag ccaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gcacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggtccgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag agttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttccggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccacc tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg tatgtgggaa gcctttcatt
    19261 cctgcttcat ccagcaatgc aagccagttg tcgcgggaat gatgcgtacg attaatgcgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgagcatc actggcatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gtttatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attaacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttgg aagagtggac tatcaggcca gacggttgcc cggaacaaac
    19921 cattcccgac gagaattcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aatgagagca ggttcaggat tccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgctt
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcatccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagca tcagcagctt
    21541 gtacggcgat gatatggtcc gtatcgctca ctgggtggca aaacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 acgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagcg
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 gcgtcgccat gcccgactgg ctaccagttt gcttgaacat ggtggggcac ttgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgtga agtttttgcc ttgccaggtc cattagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cactgaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg ttttgagcga gaagatatct acaatgccct gctatggctg gaaaaacttg
    22741 ctgattatca ggaagggttg gcagaaccga tgcaactggc ctcggatcct ctctccatgc
    22801 gtatttatac accggaagag tgtgaacgac tggatgccag ctgccgtggg tttctgcttt
    22861 tccttgagca gattcaggtg ctcaaccttg aaactcgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taacgcagag ttcgaactgg atgatctgaa atgggtgatc ctgatggtgt
    22981 tgttcaacat cccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcaaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catattgtca aagttctgga ggggcaggtt
    23281 tgccccatat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcagtaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgcc cccaatgtcg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cttgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgcgca
    23881 gcgtgaaacc attttttccc gctggccagg tccagtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gctgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggtgctgtt aataccctca
    24481 tgcggttaga agatggacgc ctgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgccctg gtttacgtat tctgcttatc ggcgctggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcgaacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 agacgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcgtactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgcg cgatctctgg
    25141 cgagagtctg gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtat gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgattgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaggatg
    26041 gtcggaacaa cgaagag
//
LOCUS       DAZGBC010000063        25889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGBC010000063 DAZGBC010000000
VERSION     DAZGBC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.063"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            474..2105
                     /gene="oppA"
                     /locus_tag="ACHK1J_002487"
     CDS             474..2105
                     /gene="oppA"
                     /locus_tag="ACHK1J_002487"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO7911738.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2191..3111
                     /gene="oppB"
                     /locus_tag="ACHK1J_002488"
     CDS             2191..3111
                     /gene="oppB"
                     /locus_tag="ACHK1J_002488"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO7911739.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3126..4034
                     /gene="oppC"
                     /locus_tag="ACHK1J_002489"
     CDS             3126..4034
                     /gene="oppC"
                     /locus_tag="ACHK1J_002489"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO7911740.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4046..5059
                     /gene="oppD"
                     /locus_tag="ACHK1J_002490"
     CDS             4046..5059
                     /gene="oppD"
                     /locus_tag="ACHK1J_002490"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO7911741.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5056..6060
                     /gene="oppF"
                     /locus_tag="ACHK1J_002491"
     CDS             5056..6060
                     /gene="oppF"
                     /locus_tag="ACHK1J_002491"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO7911742.1"
                     /translation="MNAVTEGRKVLLEIDDLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6113..6442)
                     /gene="yciU"
                     /locus_tag="ACHK1J_002492"
     CDS             complement(6113..6442)
                     /gene="yciU"
                     /locus_tag="ACHK1J_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO7911743.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6477..7937)
                     /gene="cls"
                     /locus_tag="ACHK1J_002493"
     CDS             complement(6477..7937)
                     /gene="cls"
                     /locus_tag="ACHK1J_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO7911744.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8080..8253
                     /gene="yciY"
                     /locus_tag="ACHK1J_002494"
     CDS             8080..8253
                     /gene="yciY"
                     /locus_tag="ACHK1J_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO7911745.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8307..9560)
                     /gene="kch"
                     /locus_tag="ACHK1J_002495"
     CDS             complement(8307..9560)
                     /gene="kch"
                     /locus_tag="ACHK1J_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO7911746.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9861..10157)
                     /gene="yciI"
                     /locus_tag="ACHK1J_002496"
     CDS             complement(9861..10157)
                     /gene="yciI"
                     /locus_tag="ACHK1J_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO7911747.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10381..11100
                     /gene="tonB"
                     /locus_tag="ACHK1J_002497"
     CDS             10381..11100
                     /gene="tonB"
                     /locus_tag="ACHK1J_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO7911748.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTASARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11140..11538)
                     /gene="yciA"
                     /locus_tag="ACHK1J_002498"
     CDS             complement(11140..11538)
                     /gene="yciA"
                     /locus_tag="ACHK1J_002498"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO7911749.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11643..12182)
                     /gene="yciB"
                     /locus_tag="ACHK1J_002499"
     CDS             complement(11643..12182)
                     /gene="yciB"
                     /locus_tag="ACHK1J_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO7911750.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12212..12955)
                     /gene="yciC"
                     /locus_tag="ACHK1J_002500"
     CDS             complement(12212..12955)
                     /gene="yciC"
                     /locus_tag="ACHK1J_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO7911751.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(12983..13072)
                     /locus_tag="ACHK1J_002501"
                     /pseudo
     CDS             complement(12983..13072)
                     /locus_tag="ACHK1J_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13312..13950
                     /gene="ompW"
                     /locus_tag="ACHK1J_002502"
     CDS             13312..13950
                     /gene="ompW"
                     /locus_tag="ACHK1J_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO7911752.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(14010..14516)
                     /gene="yciE"
                     /locus_tag="ACHK1J_002503"
     CDS             complement(14010..14516)
                     /gene="yciE"
                     /locus_tag="ACHK1J_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7911753.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14562..15062)
                     /gene="yciF"
                     /locus_tag="ACHK1J_002504"
     CDS             complement(14562..15062)
                     /gene="yciF"
                     /locus_tag="ACHK1J_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7911754.1"
                     /translation="MNMKTMEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(15148..15327)
                     /gene="yciG"
                     /locus_tag="ACHK1J_002505"
     CDS             complement(15148..15327)
                     /gene="yciG"
                     /locus_tag="ACHK1J_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7911755.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQNGGNKSGKS"
     gene            complement(15708..16514)
                     /gene="trpA"
                     /locus_tag="ACHK1J_002506"
     CDS             complement(15708..16514)
                     /gene="trpA"
                     /locus_tag="ACHK1J_002506"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO7911756.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(16514..17707)
                     /gene="trpB"
                     /locus_tag="ACHK1J_002507"
     CDS             complement(16514..17707)
                     /gene="trpB"
                     /locus_tag="ACHK1J_002507"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO7911757.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTKTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIEIGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(17719..19080)
                     /gene="trpCF"
                     /locus_tag="ACHK1J_002508"
     CDS             complement(17719..19080)
                     /gene="trpCF"
                     /locus_tag="ACHK1J_002508"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO7911758.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNIEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(19081..20676)
                     /gene="trpD"
                     /locus_tag="ACHK1J_002509"
     CDS             complement(19081..20676)
                     /gene="trpD"
                     /locus_tag="ACHK1J_002509"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO7911759.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(20676..22238)
                     /gene="trpE"
                     /locus_tag="ACHK1J_002510"
     CDS             complement(20676..22238)
                     /gene="trpE"
                     /locus_tag="ACHK1J_002510"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO7911760.1"
                     /translation="MQTQKPTLELLTCEGAYHDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LSFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(22260..22353)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(22330..22374)
                     /gene="trpL"
                     /locus_tag="ACHK1J_002511"
     CDS             complement(22330..22374)
                     /gene="trpL"
                     /locus_tag="ACHK1J_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO7911761.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            22512..23393
                     /gene="rnm"
                     /locus_tag="ACHK1J_002512"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             22512..23393
                     /gene="rnm"
                     /locus_tag="ACHK1J_002512"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO7911762.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDIIHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            23390..24010
                     /gene="yciO"
                     /locus_tag="ACHK1J_002513"
     CDS             23390..24010
                     /gene="yciO"
                     /locus_tag="ACHK1J_002513"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO7911763.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            24038..>25889
                     /gene="yciQ"
                     /locus_tag="ACHK1J_002514"
     CDS             24038..>25889
                     /gene="yciQ"
                     /locus_tag="ACHK1J_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO7911764.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLQFSNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTLGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQTLIRV
                     NEGVTRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFTR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWVARITPSLTAAVSAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGG"
BASE COUNT         6557 a         6281 c         6501 g         6550 t
ORIGIN      
        1 ttgttaaatg attgtgcatt tcactactgg aactgtaatc agaaaagata gacatgctta
       61 accaatctct atttgattga attgaaagat gtttgttaag acatggatgc aagctataga
      121 ttctgatacg gtcaataaaa gagaattgct taacaatttt gcaaaatgta ttggcgagta
      181 agaagcgcat ttggtacttt ccgggcaacc gccaggcgat tctttattgg taatgagaat
      241 aattaacaat taaagagcgt cgcgaaagaa taatgtgtct cgacagggga gacacagtac
      301 gaatcgacat aaggtgatcg tctgaatcac cagaataaat aaagtcggtg atagcaatcc
      361 gtaacgataa agtaacctga cagcagaaag tctccgagcc tgtgcagggt cccattccgg
      421 gattacacat gctggttaat accagtaatt ataatgaggg agtccaaaaa acaatgacca
      481 acatcaccaa gagaagttta gtagcagctg gcgttctggc tgcgctaatg gcagggaatg
      541 tcgcgctggc agctgatgta cccgcaggcg tcacactggc ggaaaaacaa acgctggtac
      601 gtaacaatgg ttcagaagtt cagtcattag atccgcacaa aattgaaggt gttccggagt
      661 ctaatatcag ccgagacctg tttgaaggct tactggtcag cgatcttgac ggccatccgg
      721 cacctggcgt cgctgaatcc tgggataata aagacgcgaa agtctggacc ttccatttgc
      781 gtaaagatgc gaaatggtct gatggcacgc cagtcacagc acaagacttt gtgtatagct
      841 ggcaacgttc tgttgatccg aacaccgctt ctccgtatgc cagttatctg caatatgggc
      901 atatcgccgg tattgatgaa attcttgaag ggaaaaaacc gattaccgat ctcggcgtga
      961 aagctattga tgatcacaca ttagaagtca ccttaagtga acccgttccg tacttctata
     1021 aattacttgt tcacccatca acttcaccgg tgccaaaagc cgctatcgag aaattcggcg
     1081 aaaaatggac ccagcctggt aatatcgtca ccaacggtgc ctatacctta aaagattggg
     1141 tcgtaaacga acgaatcgtt cttgaacgca gcccaaccta ctggaacaac gcgaaaaccg
     1201 ttattaacca ggtaacctat ttgcctattg cttctgaagt taccgatgtc aaccgctacc
     1261 gtagtggtga aatcgacatg acttataaca acatgccgat cgaattgttc cagaagctga
     1321 aaaaagagat cccggacgaa gttcacgttg atccatacct gtgcacttac tattacgaaa
     1381 ttaacaacca gaaaccgcca ttcaacgatg tgcgtgtgcg taccgcactg aaactaggta
     1441 tggaccgcga tatcattgtt aataaagtga aagcgcaggg cgacatgccc gcctatggtt
     1501 acactccacc gtatactgat ggcgcaaaat tgactcagcc agaatggttt ggctggagcc
     1561 aggaaaaacg taacgaagaa gcgaaaaaac tgctggctga agcgggttat accgcagaca
     1621 aaccgttgac catcaacctg ctgtataaca cctccgatct gcataaaaag ctggcgattg
     1681 ctgcctcttc attgtggaag aaaaacattg gtgtaaacgt caaactggtt aaccaggagt
     1741 ggaaaacgtt cctcgacacc cgtcaccagg gtacttttga tgtggcccgt gcaggctggt
     1801 gtgctgacta caacgaacca acttccttcc tgaacaccat gctgtcgaac agctcgatga
     1861 ataccgcgca ttataagagc ccggcctttg acagcattat ggcggaaacg ctgaaagtga
     1921 ctgacgaggc gcagcgcaca gctctgtaca ctaaagcaga acaacagctg gataaggatt
     1981 cggccattgt tcctgtttat tactacgtga atgcgcgtct ggtgaaaccg tgggttggtg
     2041 gttataccgg caaagatccg ctggataata cctacacccg gaatatgtac attgtgaagc
     2101 actgatggca atacgtgggg caggagtgtc ctgctccacg gtgtctgatt tttatcgcat
     2161 tacagaaggc acaggccaga aggtagggca atgttaaaat ttattttacg tcgctgtctg
     2221 gaagcgattc cgacgctatt tattcttatt actatttcgt tctttatgat gcgcctcgcg
     2281 ccgggaagtc cttttaccgg cgaacgtact ttaccgccag aagtgatggc caatatcgaa
     2341 gcgaaatatc atcttaatga tccgatcatg acacagtatt tcagctacct gaaacaactg
     2401 gcgcacggtg atttcggtcc atcgtttaaa tataaagatt attcggtcaa tgacctggtg
     2461 gcatccagtt ttcccgtttc tgccaaactg ggagccgcag catttttcct tgcggtaata
     2521 ctgggtgtta gtgctggcgt tattgccgca ttaaaacaaa acaccaaatg ggactatacc
     2581 gtgatggggc tggcaatgac cggggttgtt atccccagtt ttgtggttgc gccattatta
     2641 gtcatgatat ttgcgatcat tttgcattgg ctgccgggcg gtggctggaa tggtggggcg
     2701 cttaaattca tgatattgcc gatggtggcg ttgtcactcg cctatatcgc cagtattgcg
     2761 cgtattaccc gtggctctat gattgaagta ttacactcca actttattcg tactgcccgg
     2821 gcgaaagggt tacctatgcg gcggatcatt ttacgccacg cattaaaacc tgctctgtta
     2881 cccgtgctct cctatatggg ccccgcattt gtcggcatta ttaccggttc tatggttatc
     2941 gaaaccattt atggtttgcc ggggattggg caactgttcg ttaatggcgc attgaaccgt
     3001 gactattcct tagtgttaag cctgaccatc ctggttggtg cgttaaccat tttgtttaat
     3061 gccattgtcg atgtgctata tgcggttatc gacccgaaaa tccgttactg atactggagc
     3121 tcgcgatgat gttaagtaag aaaaacagcg agacgctgga aaatttcagt gaaaagctgg
     3181 aggtcgaagg gcgcagcttg tggcaggacg cacgtcgacg ttttatgcat aaccgtgcgg
     3241 cggttgccag tctgatagtg ctggtgctga tcgcgttatt tgtaatcctg gcaccgatgc
     3301 tttcgcagtt tgcctatgac gatactgact gggcgatgat gtccagcgcc ccggatatgg
     3361 agtccggtca ctactttggt actgactcat ccggtcgcga cctgcttgtg cgcgttgcga
     3421 ttggcgggcg tatctcactc atggtcggtg ttgctgcggc actggtggca gtggtcgtgg
     3481 ggacacttta cggttcgctt tccggttatc tgggcggtaa agtggattcg gtaatgatgc
     3541 gtctgctgga aatcctcaac tccttcccat tcatgttctt cgtcattttg ctggtgacct
     3601 ttttcggcca aaacatcctg ctgattttcg tggcgattgg catggtttcc tggctggata
     3661 tggctcgtat tgtgcgtggg caaaccctga gtctgaagcg caaagagttt attgaggcgg
     3721 cacaagttgg cggtgtatcg acgccgggca ttgttattcg ccacattgtg ccgaacgtac
     3781 tcggtgtggt ggtggtctac gcatcgctac tggtgcccag catgatcctc tttgaatctt
     3841 tccttagctt cctggggttg ggtacgcaag agccgttaag cagctggggg gcattgctga
     3901 gtgatggcgc gaactcgatg gaagtctctc catggttact gttgttccca gcgggattcc
     3961 tcgtggtgac gctgttttgt ttcaacttta tcggcgatgg cttgcgtgat gccctcgacc
     4021 cgaaagatcg ttaaggagtg cagccatgag cgtaattgaa actgcaactg tgccgctcgc
     4081 acaacaacag gctgacgcac tgctgaacgt gaaagatttg cgtgtcacct ttagtacccc
     4141 ggacggcgac gtcacggcgg tcaatgattt aaatttttcc ctacgtgccg gagaaacgct
     4201 gggcattgta ggtgagtctg gttcgggtaa atcgcaaact gcatttgcgt tgatgggcct
     4261 gttggctgcc aacggacgca ttggcggatc ggcaaccttc aatgggcgtg aaatcctcaa
     4321 tttgccagag cgtgaactca ataaactgcg cgctgaacaa atctcaatga tttttcagga
     4381 cccaatgact tcgttgaatc cctatatgcg cgtcggtgag cagttgatgg aagtgctgat
     4441 gctacataag aacatgagca aagctgaggc gtttgaagag tcggtgcgga tgctcgatgc
     4501 ggtaaaaatg ccggaagcac gtaaacgcat gaaaatgtac ccgcacgaat tttctggtgg
     4561 catgcgtcag cgagttatga ttgcgatggc attgctatgt cgacctaagc tgctgattgc
     4621 agatgaaccc actactgcgc tggacgtcac cgtacaggcg cagatcatga cgctattgaa
     4681 tgaactgaag cgggaattta ataccgccat cattatgatc acccacgatc ttggcgtggt
     4741 ggcggggatc tgcgacaaag tgctggtaat gtacgccggg cgcacgatgg aatatggcaa
     4801 cgcgcgcgat gtcttttatc aacccgttca tccttattct atcggtttgc tcaacgcggt
     4861 gcctcgtctc gatgcggaag gtgaaacaat gttgactatt ccgggtaatc cgccaaacct
     4921 gctgcgatta ccgaaaggtt gcccgttcca gccacgttgt ccgcatgcga tggaaatttg
     4981 tagtagcgca ccgccgctgg aagagtttac gcctggccgt ctgcgtgctt gctttaaacc
     5041 ggtggaggaa ctgttatgaa tgctgtaacc gaaggaagaa aagtcctcct cgaaatcgac
     5101 gatctgaaag tgcactttga aatcaaagat ggcaaacagt ggttctggca accaccgaaa
     5161 actctcaaag ccgtagatgg tgtaactctt cgcctgtatg aaggggaaac attaggtgtg
     5221 gtaggggaat cgggatgcgg taagtccacc tttgctcgcg ccatcatcgg tttggtcaag
     5281 gcgaccgacg gtcatgttgc ctggttaggt aaagagttgc tgggcatgaa gcccgatgaa
     5341 tggcgtgccg ttcgcagtga tattcagatg attttccagg atccgttggc atcgctaaac
     5401 ccgcgtatga ccatcggcga gatcattgct gaaccactgc gtacttatca tccgaaaatg
     5461 tcacgccagg aagttcgcga gcgcgtgaag gcgatgatgc tgaaagtcgg gttattgcct
     5521 aacctgatta accgctatcc gcatgagttc tccggtgggc agtgccagcg tatcgggatt
     5581 gctcgtgctc ttattcttga accgaagctg attatctgcg atgagccggt ttcggcgctg
     5641 gatgtgtcaa ttcaggcgca ggtggtcaac ctgctccagc agctgcaacg tgagatggga
     5701 ttgtcattaa tttttattgc tcatgacctg gccgtggtaa aacacatttc cgatcgtgtg
     5761 ttggtgatgt atctcggcca tgcggtagaa ctggggacct atgatgaggt ctaccacaat
     5821 ccactacatc cttacaccag ggcattgatg tcggcagtcc ccatacctga tccggatctg
     5881 gagaagaaca aaaccatcca gttactggaa ggggaattac cgtcgccgat caacccgcct
     5941 tccggttgtg tttttcgtac ccgttgcccg attgccggtc cggagtgtgc caaaacacgt
     6001 cctgtgctgg aggggagttt cagacacgcc gtttcttgcc tgaaagtcga tccgctttaa
     6061 acacaaaaag ggctgacagt tgtcagccct ttttcacgcc gaaagtgatt atttattccc
     6121 gccagatgat atggcaaagt ttgtgatctt tttcgcggca taacagaatg cgggcgaaaa
     6181 catcgttgat ttcaccatct tcactgtccg ccagaccaat caccacttcg gcaaaaaagt
     6241 ctgggttcaa atcgaaatca acatgttcct gccagtcttc cgccggatcg aataactccg
     6301 cgccgccgcg ctcttcaaac tgaagattga acagcagaac atcagctgga tcgagattgt
     6361 cggcagccag ttcgagaaaa atatcgtagg cctgctcaag cgtttcatct tcagtcaggc
     6421 gattgtttag atccatatcc atagtcacta cctgtttaac ctctgttggc gacgttttac
     6481 agcaacgggc tgaagaagta aaacagtcgc tcggcgacac gttgccataa tggacgtttt
     6541 agccataaac gggcatcgag cagacgtgaa cgcgaaatat aatcgtcctg aacggcggca
     6601 aggtctgcac caaaaccttt atcgtcgatt gccagggtaa tctcgaaatt cagccacaga
     6661 ctacgcatat caaggttaac ggtgccaacc agacttagtt cgccatcgac cagcacgctc
     6721 ttggtatgca gtaacccgcc ttcaaactga taaattttaa ccccagcagc aagcagttcc
     6781 gtaaagaatg cgcgactggc ccagccgacc agcatcgagt cattttttcg cggaaggata
     6841 atactgacat ccaccccgcg ctgcgccgcc gtgcaaatcg catgaagtaa atcatcgctt
     6901 ggcacaaagt agggcgtggt catgatcaaa tattcacgcg ccgaataagc cgcagtcaat
     6961 aatgcctggt gaatgagatc ttccggaaag ccggggccag aagcaattgt gtgaatggta
     7021 tgaccgctgg cctgctcaaa cggcataata ttgacatcag gtggtggcgg tagaatacgt
     7081 ttccctgttt caatctccca gtcgcaggaa taaataatcc ccatcgcggt ggcgatgggg
     7141 ccttccatac gcgccatcag atcaatccat tgccctacgc ccgcatcttg tttgaagtag
     7201 cgaggatcga ccatattcat gctgccggtg tacgcgatgt aattatcgat catgatcatc
     7261 ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac gcatcagatt gacctttaag
     7321 gcttcgacca cttcaatacc ggcattacgc attagctcgg gccacgggct gcggaaaaaa
     7381 gccacactcc cggcggagtc gagcatcaat cggcaatgaa tgccgcgtcg cgcagccgcc
     7441 attaatgatt cagccacctg gtccgccatg ccgccgggct gccagatata aaacaccatc
     7501 tcaatattat ggcgcgcgag ctggatgtcg cggattaacg cctgcatcac atcatctgac
     7561 tcggtcatca gttgtagctg attccctttg accccagcga tcccctgacg acgctcgcaa
     7621 agcttgaata atggcgcagc gacactgcta ttttcttcgg cgaagatatg cttacaggct
     7681 ttaaggtcgt taagccattt tgcggtggaa ggccacatcg ctctggcgcg ctcagcgcgg
     7741 cgtttgccta aatggagctc gccaacggca agataggcaa taattccgac taacggcaga
     7801 atgtaaataa tcaacagcca ggccatcgcg gagggaactg cgcgtcgttt cattagaatg
     7861 cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa tggccaacca actcaccaac
     7921 gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc gcattgttat gagtttacgc
     7981 atatggattc atctggcaaa taaaaacgcg ggaaaagcac tggtcagcgt cgggggcggg
     8041 ggtataatga ccattctgtt attgcataga gtagttaaca tgaagcggag tagaacggaa
     8101 gtggggcgct ggcgcatgca gcgtcaggct agccgacgta aatcgcgttg gcttgagggg
     8161 caatcgcgcc gaaatatgcg tatccacagc atcaggaagt gcattctaaa caaacagcgt
     8221 aactcgttat tgtttgcgat ctacaatatc taaatgttct gggcaccatt gcggtgccca
     8281 ataagtttac ttttccgtct ttaactctat ttttgcaccg attctttact gtcagctttc
     8341 gtttcttgtt catcgttatc gctgaaaatg ccatgaccgg agttcaacaa cattgaaacg
     8401 agcatatcat tattaatctc ttcaccattt aaaactcgcg ccagaatttc gctgccaaac
     8461 agttgcggtg agagaatgat atccggatgt accatcttaa tcttattcag gtttttgcta
     8521 tcactgacgg cgagaactgt tttgacatca ctgctcatat cttttgccga gagtacaaca
     8581 aacgcgttat ctgcatcgtt atcactcagc gccagaatgg cccggcaacg atcgattccc
     8641 gctttcttta atacagaact gtcattactg tcaccgggga taacgtctgc gttatcgcct
     8701 aagcgttgct caagttgctt gatatcatct tcaggcaagt tgctgataac cgttacgttt
     8761 tgtccgcgtt gattcagttg caggatcgta ttgatggcga gaatcgaatg tccgcaaaca
     8821 ataaaatgat ctttacgatg cattgtatga ttgtttcctt ttacaagttt gttgaatccc
     8881 ccgcggataa gcggaccaaa aattgaggtc atagatgtgg caaaaacggt aatgccggaa
     8941 ataatgaccg aaatagtgaa caatcgtgct gattcagaaa cagggacaat atcgccatag
     9001 ccgacggttg acatggtttc tatcgaaaaa tagaacgcgg tcatcaaact ttctattcgc
     9061 ggattaaaac cttcgcttaa ataaagcgca ccgtaggttg agtaaaacag taatgtcgtg
     9121 aaactaataa atgcaaaaat tgtcccggct gcggcgctac tgtgggagaa gtctttgcgc
     9181 agtatcagca aaaacaccag cgtaaaaatg caaaatccaa tactaaattt cagccaggga
     9241 taaaaatgca gggtgtaaat tagcgctatc aacaacaaaa tgatactgat tgcccatgcg
     9301 agctttgccc ggaacagtaa gccaatggag ttcaggacaa gaaagacgcc gagcataaat
     9361 agtggggcgt ttgccagcaa ggagagatcc aactctgaaa atgctttgat atggaaaata
     9421 tcaagcaaat tgaccgacat accatagatt gttttaaaaa taagcaatcc atttaaaaag
     9481 acggccagcg cgagaatgtc gtgccgtagg gtcacccata aatttgttgc tgtttgtttg
     9541 aatgtagccc agtgactcac tgatacctct gtaaccacaa ttttgtttaa ttatcttagc
     9601 gtgcacattt tctgaacata catgcagcgc gtgaatgtgt taaacaatag tagatgactt
     9661 tatccgcagt aatcaaaggt tttgagtgag caactctgcg tcaggaaagg gcatttttcc
     9721 tgaatatgct cacatcatat aaagaaatac agataaagtt attatctgct tgtggtggtg
     9781 aatgcactga ccgacctata aggaaaggcc aaacaagaac acggttgcaa aaaccgtgcc
     9841 cttaaatatt ggctctctac tcagaacact ttcttaaatg gtttcactga aacgtgttca
     9901 tagactcctg ccgctacgta cgggtcagca tcggcccagg cctgagctgc ttccagcgat
     9961 tcaaattcag caataacggt tgagccagta aatcccgcag cccctggatc gttactgtct
    10021 accgctggca ttggaccagc tgtcaacaaa cgaccttcat catgcagtaa ctgtaaacgt
    10081 gctaaatgtg ccggacgaac ggaaaggcgt ttttcgaggc tatcagcttt gtcttgagcg
    10141 taaataacat acaacacggg cacaactcct tgttcgggaa agttgtaaag tacgttattt
    10201 gaaagggcga agatctgcaa cggaaagatg atgtctttgt taagaccatg cataaagtaa
    10261 ggtcaattac gccaaaaatg acattttcac tgatcctgat cgtcttgcct tattgaatat
    10321 gattgctatt tgcatttaaa atcgaggcct ggtttttcta ctgaaatgat tatgacttca
    10381 atgacccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcatacat
    10441 ggtgctgttg tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg
    10501 cctgcgcagc caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc
    10561 gttcagccgc caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa
    10621 ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg
    10681 aagccggtga aaaaggtaca ggagcagcca aaacgcgatg tcaaacccgt agagtcgcgt
    10741 ccggcatcac cgtttgaaaa tacggcatcg gcacgcccga catcaagtac ggcaacggct
    10801 gcaaccagca agccggttac cagtgtggct tcaggaccac gcgcattaag ccgtaatcag
    10861 ccgcagtatc cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt
    10921 gatgtcacgc cggatggtcg cgtggataac gtgcaaatcc tctcagctaa acctgcgaac
    10981 atgtttgagc gtgaggtgaa aaatgcgatg cgcagatggc gttatgagcc gggtaagcca
    11041 ggcagtggga ttgtggtgaa tatcctgttt aaaattaacg gcaccaccga aattcagtaa
    11101 gcagaaagtc aaaagcctcc gaccggaggc ttttgattat tactcaacag gtaaggcgcg
    11161 aggttttcct tcaggatcaa ccgcgacata cttaaataat gcttctgtcg ctttatagcg
    11221 ttgcccgatt ggttcagacg ctactttttt cacccacact tcaatattaa tgctgaccga
    11281 tgtcgtccct ttctggacac agcgtgcata gcagcacacc acatcgccga ccgcaaccgg
    11341 ccgtaagaaa gtcattcctt caacccgcac agtcactacg cgaccgtggg caatttcttt
    11401 cgccagaata gcgccgccaa tatccatttg tgacattaac caaccaccaa agatgtcacc
    11461 attggcattg gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc cctgagggac
    11521 gttatgtgtt gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac
    11581 tatgattttc ctgaggagaa cagaggagat acagcgccag ccccgaagga ctggcagtct
    11641 ggttaggatt tatcttcctg cggcatgtgg cggtagatat agataccgct taacaatgtg
    11701 aagattaagg taagggcggt caggccaaag actttaaagt tgacccaaat attttgcggc
    11761 agccagaatg cgatgtagat gtttgccaga ccgcaaagga taaagaaaac agcccaggcc
    11821 agattcagct tcgaccatac cggttgcggc agcgtgagtt ctttacccag catccgctga
    11881 attagcggct ttttcatcac ccattggctg actaacaggg cacccgcaaa cagggcataa
    11941 atgacagtaa ccttccattt aataaactca tcattgtgga agaacagcgt caagccaccg
    12001 aagacgacca ccagaacaaa agtgatcagg gccatcttct caaccttacg aaagcgaacc
    12061 cagctataga taagcacaat cgccgtggcg acgatcagcg ccgcagtagc cgcatagatg
    12121 tcataaatct tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag aaactgcttc
    12181 attttacgat tccgtaatca aatgcttaag gttattggcg aatcaacata tacagacgga
    12241 acagatagat gagcaatacg gctgaaatca ggttgctcaa ggtgttcgcc agtacggcac
    12301 caatttccgg ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt gtctttgcca
    12361 gcaaccagct cagtactgcg ggcgccacca gacgcatatt cgcccaagtc agccgcatac
    12421 tgctacgcat cgaggcaaaa acgcccattt tgtcctgtac caacatcacc ggtgccaggg
    12481 ccagtaaaat ggccatgata attcccggaa cgaccactag catgatgcca atctgtacta
    12541 aaagggtagt cagaaaaatc agaataaata actttggcaa tatcggcgca ctggcaccaa
    12601 tagctcgcag cgcactgact ctctgacccg cagacaccag ctggataatt aatattacgc
    12661 ctccggcgag aatggcgtta ccgattaatc ctgaaaaagt ggacgccgct gaagcctgca
    12721 gcaaaatttg ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac ccactactgc
    12781 cgctaacggg cacgccgtca ttgagctgcg caagctgtgc atcactgggt gagaaaacat
    12841 gccctaacac cactgtgata aacgcacata gcaacgatac caacagaatg gtcataaatt
    12901 gattacggaa gaaatttccg gtgtcacggt atacggactg cgccgtgata gacatgcact
    12961 ctccttgagt tttatgcagg tgttaattag cgggcaattg taccctggtt aagcctcggg
    13021 tggcagcatc aggtcttgta tggaaaagca tatctttgta aagcggaggt aattgctggc
    13081 cttcaggcgt gggcaaaccg cggcaagtgg cgatcgagtt aatatttgcg tagcgaaaat
    13141 atttaaaaat tgatttaaat cacgttaacc aggattccca atgcaacttc taaattaatc
    13201 cagatcaata aagggtgaat tatcatatgt aatgtgatct atgtaggatc atttgttact
    13261 ccaatgtagg tatatttgtc acgtttttat aaccataacg acggagcgga tatgaaaaag
    13321 ttaacagtgg cggctttggc agtaacaaca cttctctctg gcagtgcctt tgcgcatgaa
    13381 gcaggcgaat tttttatgcg tgcaggttct gcaaccgtac gtccaacaga aggtgctggt
    13441 ggtacgttag gaagtctggg tggattcagc gtgaccaata acactcaact gggcctgacg
    13501 tttacttata tggcgaccga caacattggt gtggaattac tggcagcgac gccgttccgc
    13561 cataaaatcg gcacccgggc gaccggcgat attgcaaccg ttcatcatct gccgccaaca
    13621 ctgatggcgc agtggtattt tggtgatgcc agcagcaaat tccgtcctta tgttggggca
    13681 ggtattaact acaccacctt ctttgataat ggatttaacg atcatggcaa agaggcgggg
    13741 ctttccgatc tcagtctgaa agattcctgg ggagctgccg ggcaggtggg ggttgattat
    13801 ctgattaacc gtgactggtt ggttaatatg tcagtgtggt acatggatat cgataccacc
    13861 gccaattata aactgggcgg tgcacagcaa catgatagcg tacgcctcga tccgtgggtg
    13921 tttatgttct cagcaggata tcgtttttaa ttccgcacaa aaacgacccc gtaatatacg
    13981 gggtcaataa ggacatggca taaagggtat tatttcttcg cttctacgcc atcagtttca
    14041 gagcgaatta aaaatttctc agttgtttgc ggaatatgct gaatcagcca gtcggccatt
    14101 tgcttttcct catttaaaat cgcttcgatg atcggaattg aggctgtatc accggcattt
    14161 tttgctgctg ctaatagaga tgtgtaacag gcgatttcaa attgctcgaa gacatatccg
    14221 ctaatagaac ctttgactat ttcatcagaa gggaatatac caccgattga ctgcccaagc
    14281 gcagccattt tactcatgga atctttaatg actgaacgtg aaatgtcatt acgatcaaga
    14341 atagtttcca gttgaacaat ctggtttttg gtttcactaa gatgttgttc aatacgagca
    14401 cgtagttcag gataattatc tatacggctg gccatggatt caagcataga ttcggcttgc
    14461 ttttccattg catgggcgtc acgtaaccag tcatgataat gttcaatacg attcatgaaa
    14521 atgtcctctt tataaaatga ctaaaagtta aaattcatat ttcaggcttt attttcggct
    14581 ttcttattta cgttattaag ggccagatca gtcagtttga tgtcggtggc cttttcttct
    14641 tccagggtct ctttcagaag cttcgctgct ttacggtaac ctaattgttc agccagcgtc
    14701 gctaatgtcc cgtaactggc aatctcataa tgctcgactt tctgtgctgc ggcaatcagt
    14761 gcggcatcac gcacttcgtt tttctcggta ctttcgatga cctcattagc ttcttcaata
    14821 agaccttcca ttgccacaca tttcatgcgc ttaattttca gattcgattc tgattccaca
    14881 acttgatcaa tacgttcaat ctgtccatgt gtttcctcga ggtgcgcatg aaaagcctga
    14941 cttaattttt cgtttgatgt tgctcttgcg agttttgcca gtgcccgggt taattgtttt
    15001 tctgcgctgt aggtatctga aagtaggtga ataaatacat cttccatggt cttcatattc
    15061 atatttttct ccagtggaat cactgcgagc catacggccc gcagaagcaa ttatctttat
    15121 cagctgatat taataattaa cggtgaatca ggatttacct gatttattac caccgttttg
    15181 ttgaccgcct tttttacccg cttcagatgc gcgttgcgga tcatttttaa aattaccgcc
    15241 gctatgctga ccgcctttac ggcctgcgtc ggatgccttc tcacggtctt cggcgaaatt
    15301 tcctgaacca ccacgatgtt cggccatgtt atttctcccg ttgcgttgca ttgtttcatt
    15361 aatatgagtg ttgtgtgtcg acactcatta aaattagtcg ataatgagaa ttggtcaaat
    15421 taagcgtaac gagaaggaag tgggaaaata tattatgagg aacgttcttg atatattaac
    15481 aattgcctga ggaataagtg acttagagaa aattattgat tttactggtg ttatgttgcg
    15541 ggattaattt gtttgattat attactgttg ggcggaaaaa tgacgtaagt tgacgttcga
    15601 caggggtaag cgaaacggta aaaagataaa tattaaatga atttaggatt tttccggctt
    15661 cgttaaagaa agttaaaatg ccgccagcgg aactggcggc tgtgggatta actgcgcgtc
    15721 gccgctttca tcggttgtac aaaagctttc agtgccgcca gcattttctc tggctcatta
    15781 atatgttgct cgatgatttt aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca
    15841 tcaatcgctg cttttacctg atccggggcg gaaataccaa atccctgcaa tggaggtgca
    15901 gcgttgtact ctttcagctt cgcaaccaga tgattgaggg gtaacgcggc gcggttttct
    15961 gcgccggtca cgcccgctcg tgacagcaaa tatgtgtaac cacgaccgta agaggctatc
    16021 tggcgcagca ggtcgtcatc ggcatttggc gggcagatga agataggtgc gacattatga
    16081 cgcaacgcgg cctggcggaa gggcgcggac tcttcaactg gcacatcggc aaccagcacc
    16141 gaatcgacgc cgactttttc gcactgggca taaaactcat caatgccttt gttaaacacc
    16201 agattggcat acatcaacag gccaatggga atggtcgggt gtttctggcg aatcagtgcc
    16261 agcatttcaa aacattgtgc cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt
    16321 tgaatggtcg ggccatccgc cagtgggtcg gagaagggga tacctaactc cagcgcgtca
    16381 gcaccggctt caattagcgt atcgataatt ttcaatgact gctcaatgcc cggatccccg
    16441 agcgtgacga aaggaacgaa tgcgccttct ttgcgctcct tcaactgggc aaacagagat
    16501 tcgtagcgtt ccatcagatt tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt
    16561 ctttatcgcc gcgaccggaa aggttaacca ccagtagctg ctctttttcc gggttttcgc
    16621 gcatcatttt cagtgcatgg gccagggcgt gggaggattc cagcgccggg atgatccctt
    16681 cgtgcaggca cagcgttttg aaggcttcaa gggcttcatc atcggtaata gacacgtaat
    16741 cagcgcgtcc agtgctgtta agatacgcgt gttgtgggcc gacggacggg aaatccagcc
    16801 cggcggagat ggagtaagat tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt
    16861 tcataccgaa atagatgccc acgcgaccat gttttaacgg tgcgccgtgc tcgccgattt
    16921 ctataccgtg accgccaggc tccacaccaa tcaggccgac gttggtttcg ttgatgaaat
    16981 ctgcaaacat gccgatggca ttcgaaccgc cgccaacaca ggcgataacg gcatccggca
    17041 ggcgaccttc tctttccaga atctgtgctt tggtttcttc gccaatcatc cgctgaaact
    17101 cacggacaat ggtcggataa ggatgcgggc cagctgcggt gcccagcata tagtgcgcgg
    17161 tttcataact gccggaccag tcgcgtagcg cctcgttaca ggcatctttc agcgtcgcgg
    17221 aaccgctatg caccgggatc acttccgcac ccattaagcg catacgaaaa acgtttggcg
    17281 actggcgttc aacgtctttg gcccccatat aaatacggca tttcaggccg agcagggcgc
    17341 tggcaagggc cgacgccacg ccatgctgac cggccccggt ttcggcgatg atttcggttt
    17401 tacccatccg cttcgccagt aaagcctgac cgagcacctg gttagtttta tgcgcgccgc
    17461 cgtgcagcaa atcttcgcgc ttcagataca gcgtggtttt cgtcccagcc gtaatgttct
    17521 ggcatttggt cagtgcggtt ggacgcccgg catagttttt cagtaggtcg ttgaactggg
    17581 cctgaaattc aggatctttt tgcgcgctga caaaagcttc ttccagctgg cgcagagcag
    17641 gcatcaggat ttgtggcacg tacatgccgc caaactcacc aaaatagggg ttaagtaatg
    17701 ttgtcattgt tcctttcctt aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg
    17761 tgcgtctttg atgcccggtt gcgactctac agcagaatta aaatcaagtc cggcgcagcc
    17821 ggtttgtgcc gcttccacgc agttatctgc gcctaagccc cccgccagca gaacgttgcc
    17881 aagcgattga ccgtttaaca gtgaccagtc gaaacgttgc ccgcttccac cctggccgtt
    17941 gtctaaaaca tatttatcaa cgtgctgaag ctcgcgggcg ggcagggttt caccgacgct
    18001 taatgctttc cagatggcaa cgtgtgctgg cagagcttca cgcagcgtat cgatatacag
    18061 ctgatcttca ttaccatgca gttgcactgc cgccagcgat aacaccttag ctttgtccac
    18121 cacatcggca atatcgtgat tgcggaacac gccaacatac tgcaacggtg ccgcagccat
    18181 cacttcctgc gcctgttcaa tgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc
    18241 accgtaaatc gcgcccgcgt catacgctgc tttagcatct tgcccacgcg tcaggccaca
    18301 tactttattc tcacccagca acacccggcg cacggcggcg ttcaaatcgt catgggccat
    18361 caacgccgaa ccaatcagaa aaccgttagc gaagtggctt aactcgcgca cctgagcgta
    18421 agtattgatg cctgattcgc tgattaccgt cacgttgtgc cccagtttcg gcgcaagctc
    18481 gcgggtacgg ttgagatcaa tcgacaaatc acgcagatcg cggttgttga tgccaacgac
    18541 ctttgccccc aatgcaatgg cgcgctccag ttcctcttca ttactgactt cggtcagcac
    18601 acccatctcc agactgtggg cgacggcggc aagctggcga tattgttcgt catccagtac
    18661 tgaaagcatt aataagcagg catcggcctg gtaatagcgc gccagataga tctggtaagg
    18721 gtcgataatg aagtctttac ataaaatcgg ctgcggggcg atttggctga cgatggggag
    18781 gaaatcaaag ctcccctgaa aatatttctc atcagtcagc accgaaattg ccgaagcgta
    18841 atgtttataa atggcggcaa tgcgtgctgg atcgaaatca tcacggatca cgccttttga
    18901 cggcgacgct ttcttgcact ccagaataaa cgccgtgcgc gcaccctgta gcgcatcata
    18961 aaaatgtcgc gtgctcggct gaacctcatt ctgaaaactg gccagcggtt gctgctgttt
    19021 gcgggcttct acccaaatcg ccttgtctgc gacgatcttc gctaaaacgg tttgcatcat
    19081 ttaccctcgt gccgccagtg cggtgactct gtcgtaagcg gaaccactgc gcagtacctc
    19141 aagaacggtt tgcgcattgg cttgcagatc ttcatggcca tgcaggcgca ttaacatggc
    19201 gacgttcgca gcgacggctg cttcatgggc ggcgtcgcct ttaccttgta acaaacgtgt
    19261 taaaatgtca cggttttctt ccggtgttcc gcctgccagt tgctcctggt ggtagggcgt
    19321 caggccaaaa tcttcagcgg tcaattgata gctcttaatt tcgccgtcat gcagctcggc
    19381 aacgattgtc ggcgcgtgta atgaaacttc atccatcccg ccgctgtgca ccaccgccgc
    19441 gcgttgatac cccagcacgc gcaaggtttc ggcaatcggc agcaccagtt ccggactata
    19501 aacaccaatt aacgccagcg gcggatgcgc cgggttaatc aatggtccca gcacgttgaa
    19561 caaggtgcgg gttttcagtt gctggcgaac cggcatcgcg tggcggaatc cggtgtgata
    19621 cttcggcgca aagaggaaac agacgcctaa ttcgtccagc gcctggcgcg atttatcggc
    19681 gttcatatca agattaatac cgaatgccgc cagcagatcc gacgagccag atttactgga
    19741 gacgctacgg ttgccgtgtt tcgcaacttt cagcccacag gccgcggcga caaacgcact
    19801 ggcggtggaa atattgatac tgttgctgcc gtcaccgcca gtcccgacga tatcagcaaa
    19861 cagataatcc gggcgcggga acggcgctgc gttttccagt agcgcggttg ctgctccggc
    19921 gatctcgttc gggtgctcac cgcgaatttt catgctcacc agcgccgccg ccagttgttc
    19981 cggcttcagc tcgccacgca ccaccgctga aaacagctgg tgactttctt gttggctaag
    20041 cgtctgcgcc tgatacagtt tttccagaat cggttgcagc gtgttggttg gctcaagttt
    20101 ctgctgcgcc caggccagcg tttgttccag caggcgagcg ccctgggtag taagaatgga
    20161 ttccggatgg aactggaatc cacaaacgcg atccgcatcg tgacgtactg ccatcaccat
    20221 gccattaaaa tgggcattga tggttaaacc tgccggaatg ttactgccaa ccagcgagtg
    20281 ataacgcgcc accggcaatg ggtttgttaa tccggcaaac atcgcctgac cgtcgtgttc
    20341 aatgctggag gctttaccgt ggagaatttc gcccgcctga ccgacatagc ccccgtaagc
    20401 ttcgacaatc gcctgatgac caaggcaaat gccaataatc ggcagcttac cgcgcaggcg
    20461 ggtaagcagt tctggcatac aaccagcttc gctcggcaca ccgggtcctg gagaaagcat
    20521 cagcaccgga ttgctcatcg tcgccaggcg ttcaattaag gtttgcgccg gaatatggtt
    20581 gcggtaaatc accacgttat gcccattgct gcgcaactga tctgccaggt tgtacgtaaa
    20641 agagtcgata ttatcgagca gcagaatgtc agccatcaga aagtctcctg tgcatgatgc
    20701 gcggtggcaa tagcgcgcag tacagcgcgg gctttattac gggtttcgtc ggcttccgac
    20761 tgcggaacag aatcaaggac tacgccagca ccggcttgca cggtggcgat accgttttcc
    20821 accagcgccg agcggatcac aatgcaggtg tcgagatcgc catgcgcggt gaaataacct
    20881 accgcgccgc cgtagctgcc gcggcgacga ccttcagcct cggcaattaa ctgcatagcg
    20941 cgtactttcg gcgcaccgct taacgtaccc atattcatac aggcgcggta agcgtgtagg
    21001 gcgtcgagat cgtggcgcag ctcaccgacc acgcgggaga ccaggtgcat cacgtaagag
    21061 taacggtcaa ctttggtaag atcggcgacg tagcggctgc cgggggtgca aatgcgtgcc
    21121 agatcattac gggcgagatc caccagcatc agatgttcag aaagctcttt atgatcggta
    21181 cgcatttcca gttcgatgcg gctgtcgagg tctctgtcca gtgaaccatc ggcacgacga
    21241 ccgcgtggac gtgttccggc aatcgggtag atctcaatct ggcggctggt ggcgtcatac
    21301 ttgagcgaac tttccggcga cgcgccaaac agggtgaaat cattatcctg cataaaaaac
    21361 atgtacgggc tgggattact ctttttcagc acgtaatagg ccgccagcgg tgacgggcag
    21421 ggcagagaga aacggcgaga tggcaccacc tggaaaattt ctccggcgcg aatcgctttt
    21481 tgcaacaaac gcactacgcc accgaactct tcatcgctct ggttacattc acaacgcata
    21541 tgcggcacgg aaaccaccgg cagcggtggc gcggtttcgg tcagttgctg gcgaagatcg
    21601 ttcaggcgag cagtgagacg ttgtttttct tcttcattcg gagcaaaaag gctggcctga
    21661 atgcgggtgc tttttttctg atggtcaatc accatcagcg tttcagcgag ataaaaacag
    21721 aaatcagggc agttattttc cgctgacagt tgcggtaaat cttcaaaccc cgccacaagg
    21781 tcataagaga acaggccgcc gaagaacatg gcttctcgtt cttccttcgg tacattcaac
    21841 agattctgca ataagcggaa agcgtcaaaa accgaaaggg agcataagcg agcgtcttca
    21901 tccagcagtg gactaacagg ggggaagctc agcacacggc agtttggtaa ttgttcattt
    21961 tccacacccg caggcagggc gttatccagt agtgtcagca gggcttcgcc gttgccggaa
    22021 agggactgga ttgtgacagt gtcacctaaa gctgtaatgc gcagcgcact gtctaccagc
    22081 agcaggcttt ttaaatcatc tttgctgtcg atatctgcgg attccagcag cagcgttgcc
    22141 ggacgattcc cacacaactg gtgaaaaagc gcggtgggat tgtcgtgata agcgccttcg
    22201 caggttagca gttcgagagt cggtttttgt gtttgcattg ttattctcta attttgttca
    22261 aaaaaaagcc cgctcattag gcgggctggg tatctgattg ctttacgcat ggtgaataca
    22321 ctgcccgttt caggaagtgc gccaccaacc tttcagtacg aaaattgctt tcattgtcga
    22381 tacccttttt acgtgaactt gcgtactagt taactagttc gatgattaat tgtcaacagc
    22441 tcatttcaga atatttgcca gaaccgttat gatgtcggcg caaaaaacat tatccagaat
    22501 gggagtgcgc cttgagcgac acgaattacg cagtgattta cgacctgcac agccatacca
    22561 cagcttccga tggctgcctg acgccagaag cattggtgca ccgtgcagtc gagatgcgcg
    22621 tcggcaccct ggcgatcacc gaccatgaca ccacagctgc aatcgcgcca gcaagagagg
    22681 aaatttcacg ttcgggactg gcactgaatc ttattcccgg cgtggaaata tccacggtct
    22741 gggaaaatca tgaaattcat attgtcgggc tgaatattga tattattcat ccgctaatgt
    22801 gtgagttcct cgcgcagcag acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa
    22861 gacttgaaaa agcgcaaata cctggggcgc tggaaggcgc acaacgactg gcgcagggcg
    22921 gcgcagtgac gcgcggtcat tttgcgcgtt tccttgttga gtgcggcaaa gccagttcaa
    22981 tggcggatgt ctttaaaaag tatctggcgc gcgggaaaac cggatacgtt ccgccacagt
    23041 ggtgtacaat agaacaagct attgatgtca ttcatcattc tggcggtaag gcggtgctag
    23101 ctcatcctgg gcggtacaat cttagtgcta aatggctgaa aagattggtt gcgcattttg
    23161 ccgaacacga cggcgacgcg atggaagtcg cgcagtgcca gcaatcgccc aatgaacgta
    23221 cccagctggc gacgcttgcc cgccagcacc atttatgggc atcacaagga tctgattttc
    23281 atcagccatg cccgtggatc gaactgggtc gtaaactctg gctgcccgca ggcgttgaag
    23341 gcgtctggca gctatgggaa cagccgcaga acaccacaga gagggaatta tgagccagtt
    23401 tttttatatt catcctgata acccgcagca acgtctgatc aaccaggcgg tggagatcgt
    23461 gcgtaaaggc ggggtgattg tttatccaac cgattccggc tatgcgctcg gctgtaaaat
    23521 tgaagataaa aacgcgatgg agcgtatttg tcgtattcgc cagctgccgg acggtcacaa
    23581 ctttaccctg atgtgtcgcg atctttctga actctcgacc tattcatttg tcgataacgt
    23641 tgcgtttcgt ttaatgaaaa acaacacgcc gggcaactat accttcatcc tgaaggggac
    23701 gaaggaggtg ccacgccgtc tgttgcagga aaaacgcaaa accatcggta tgcgtgtgcc
    23761 ttctaacccg attgcgcagg cgttgcttga agcactgggc gaaccgatgc tttccacttc
    23821 gctaatgctg ccaggcagcg aatttaccga atccgatccc gaagaaatta aagaccgtct
    23881 ggaaaaacag gtagatttga ttattcacgg cggctatctc gggcagaaac cgacaacggt
    23941 tattgatctt accgatgata cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc
    24001 tttcttataa caaattgcgt tccacggatg gaagactatg gcagggaagt ttcgctgcat
    24061 tttgctattg atagttgggc tttttttctc ttcgttaagt tatgcgaaaa acacggagat
    24121 gccttcttat gaagaaggga tctcgctctt tgatgttgaa gccactctgc aaccggatgg
    24181 ggtgctcgac atcaaagaga acattcattt tcaggcgcga aatcagcaga ttaagcacgg
    24241 attttatcgt gatttaccac gactatggat gcagcctgat ggggacgctg cactgctgaa
    24301 ctatcatatt gttggcgtca cccgtgatgg tattcctgaa ccctggcatc ttgactggca
    24361 tatcgggtta atgagtattg tcgtgggcga taaacaacgt ttcttgcctc aaggcgacta
    24421 tcattatcaa attcattatc aggttaaaaa tgctttcctg cgtgaggggg attctgatct
    24481 gctaatttgg aacgtgaccg gtaaccactg gccgtttgaa atttataaga cccgtttttc
    24541 tctccagttc tctaatattg cgggtaatcc atttagcgaa atcgatctct ttaccggaga
    24601 agagggcgac acatatcgta atggccgcat ccttgaggac ggaagaattg aatcccgcga
    24661 tccgttttat cgtgaagatt tcacggtact ctaccgttgg cctcactcgt tacttagcaa
    24721 tgccccggct ccacaaacga cgaatatttt cagccatctt cttttaccct ccacgtcatc
    24781 gttgttaatt tggtttccgt gtctattcct ggtttgtgga tggttatatc tctggaagcg
    24841 caggccgcaa tttacgccgg tagatgtgat tgaaaccgat gtcattccgc cagattacac
    24901 gctcggcatg ttacgtctcg atgcgaagct ggtttacgac gataaaggtt tttgtgccga
    24961 tatcgtaaat ctgatagtga aaggaaaaat tcatctggaa gatcagtctg acaagaacca
    25021 gcaaaccctg attcgtgtta atgaaggcgt gaccagaaat aatgcggtat tactgcccgc
    25081 agagcagtta ttactggaag cgttatttcg taaaggcgat aaggtcgttc ttacggggag
    25141 acgcaacaga gtcttacgca gggcattttt acggatgcag aaattttatc tgccgcgtaa
    25201 aaagtcttcg ttttaccggc ctgatacgtt tttgcaatgg ggtggactgg caatattggc
    25261 ggtcattctc tacggtaacc tgagtcctgt aggctgggca ggaatgagtc tggtgggcga
    25321 tatgtttatt atgatctgct ggcttcttcc ttttttattt tgttcccttg agcttttgtt
    25381 tacccgcgat gatgacaagc cttgcgttaa tcgtgtaatc atcactttgt ttttaccgct
    25441 gatttgttca ggcgtggcct tttattctct ctatatcaat gtcggagatg tattctttta
    25501 ctggtatatg ccagcggggt attttagcgc tgttttcctg accggttatc tcactggtat
    25561 ggggtatatt tttctgccaa agtttaccca aactgggcag caacgttatg cccacggtga
    25621 agctatcgtt aactatcttg cgcgtaaaga ggcggcaaca cacagtgggc ggcggcggaa
    25681 aggggaaaca cggaaactgg attacgcgtt gctaggttgg gcagtctcag caaaccttgg
    25741 aagagaatgg gttgcacgta tcaccccatc actcacagcg gctgttagcg ccccggaaat
    25801 tgcccgtagt ggcgttttgt tctcattaca gatgcacctg agtctggggg ccaataccag
    25861 tttgttgggg cgaagttatt ccggtggtg
//
LOCUS       DAZGBC010000064        25880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGBC010000064 DAZGBC010000000
VERSION     DAZGBC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.064"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..193)
                     /gene="shoB"
                     /locus_tag="ACHK1J_002515"
     CDS             complement(113..193)
                     /gene="shoB"
                     /locus_tag="ACHK1J_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO7911765.1"
                     /translation="MTDCRYLIKRAIKIIIAVLQLILLFL"
     gene            complement(614..994)
                     /gene="acpS"
                     /locus_tag="ACHK1J_002516"
     CDS             complement(614..994)
                     /gene="acpS"
                     /locus_tag="ACHK1J_002516"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO7911766.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(994..1725)
                     /gene="pdxJ"
                     /locus_tag="ACHK1J_002517"
     CDS             complement(994..1725)
                     /gene="pdxJ"
                     /locus_tag="ACHK1J_002517"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO7911767.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRNACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1737..2465)
                     /gene="recO"
                     /locus_tag="ACHK1J_002518"
     CDS             complement(1737..2465)
                     /gene="recO"
                     /locus_tag="ACHK1J_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO7911768.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2477..3382)
                     /gene="era"
                     /locus_tag="ACHK1J_002519"
     CDS             complement(2477..3382)
                     /gene="era"
                     /locus_tag="ACHK1J_002519"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO7911769.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3379..4059)
                     /gene="rnc"
                     /locus_tag="ACHK1J_002520"
     CDS             complement(3379..4059)
                     /gene="rnc"
                     /locus_tag="ACHK1J_002520"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO7911770.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4332..5306)
                     /gene="lepB"
                     /locus_tag="ACHK1J_002521"
     CDS             complement(4332..5306)
                     /gene="lepB"
                     /locus_tag="ACHK1J_002521"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO7911771.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5322..7121)
                     /gene="lepA"
                     /locus_tag="ACHK1J_002522"
     CDS             complement(5322..7121)
                     /gene="lepA"
                     /locus_tag="ACHK1J_002522"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO7911772.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7319..7798)
                     /gene="rseC"
                     /locus_tag="ACHK1J_002523"
     CDS             complement(7319..7798)
                     /gene="rseC"
                     /locus_tag="ACHK1J_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO7911773.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7795..8751)
                     /gene="rseB"
                     /locus_tag="ACHK1J_002524"
     CDS             complement(7795..8751)
                     /gene="rseB"
                     /locus_tag="ACHK1J_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO7911774.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8751..9401)
                     /gene="rseA"
                     /locus_tag="ACHK1J_002525"
     CDS             complement(8751..9401)
                     /gene="rseA"
                     /locus_tag="ACHK1J_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO7911775.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9434..10009)
                     /gene="rpoE"
                     /locus_tag="ACHK1J_002526"
     CDS             complement(9434..10009)
                     /gene="rpoE"
                     /locus_tag="ACHK1J_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO7911776.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(10006..10161)
                     /gene="rseD"
                     /locus_tag="ACHK1J_002527"
     CDS             complement(10006..10161)
                     /gene="rseD"
                     /locus_tag="ACHK1J_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO7911777.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10417..12039
                     /gene="nadB"
                     /locus_tag="ACHK1J_002528"
     CDS             10417..12039
                     /gene="nadB"
                     /locus_tag="ACHK1J_002528"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO7911778.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERN
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVHGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12024..12761)
                     /gene="trmN"
                     /locus_tag="ACHK1J_002529"
     CDS             complement(12024..12761)
                     /gene="trmN"
                     /locus_tag="ACHK1J_002529"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO7911779.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12893..14227
                     /gene="srmB"
                     /locus_tag="ACHK1J_002530"
     CDS             12893..14227
                     /gene="srmB"
                     /locus_tag="ACHK1J_002530"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO7911780.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14260..15141)
                     /gene="yfiE"
                     /locus_tag="ACHK1J_002531"
     CDS             complement(14260..15141)
                     /gene="yfiE"
                     /locus_tag="ACHK1J_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7911781.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            15244..15831
                     /gene="eamB"
                     /locus_tag="ACHK1J_002532"
     CDS             15244..15831
                     /gene="eamB"
                     /locus_tag="ACHK1J_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO7911782.1"
                     /translation="MTPTLLSAFWTYTLITALTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISYSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15886..16269)
                     /gene="grcA"
                     /locus_tag="ACHK1J_002533"
     CDS             complement(15886..16269)
                     /gene="grcA"
                     /locus_tag="ACHK1J_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO7911783.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16574..17263
                     /gene="ung"
                     /locus_tag="ACHK1J_002534"
     CDS             16574..17263
                     /gene="ung"
                     /locus_tag="ACHK1J_002534"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO7911784.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17311..18348)
                     /gene="yfiF"
                     /locus_tag="ACHK1J_002535"
     CDS             complement(17311..18348)
                     /gene="yfiF"
                     /locus_tag="ACHK1J_002535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7911785.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18555..18974
                     /gene="trxC"
                     /locus_tag="ACHK1J_002536"
     CDS             18555..18974
                     /gene="trxC"
                     /locus_tag="ACHK1J_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO7911786.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19043..19741
                     /gene="tapT"
                     /locus_tag="ACHK1J_002537"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19043..19741
                     /gene="tapT"
                     /locus_tag="ACHK1J_002537"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO7911787.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAEAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19773..22433
                     /gene="pat"
                     /locus_tag="ACHK1J_002538"
     CDS             19773..22433
                     /gene="pat"
                     /locus_tag="ACHK1J_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO7911788.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22547..23902
                     /gene="pssA"
                     /locus_tag="ACHK1J_002539"
     CDS             22547..23902
                     /gene="pssA"
                     /locus_tag="ACHK1J_002539"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO7911789.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23948..24271
                     /gene="yfiM"
                     /locus_tag="ACHK1J_002540"
     CDS             23948..24271
                     /gene="yfiM"
                     /locus_tag="ACHK1J_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO7911790.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24268..25566)
                     /gene="kgtP"
                     /locus_tag="ACHK1J_002541"
     CDS             complement(24268..25566)
                     /gene="kgtP"
                     /locus_tag="ACHK1J_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO7911791.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25683..>25880)
                     /locus_tag="ACHK1J_002542"
     CDS             complement(25683..>25880)
                     /locus_tag="ACHK1J_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911792.1"
                     /translation="KRKAILTDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHL
                     HTCSPHQKFILKLLNNHFPAN"
BASE COUNT         6256 a         6839 c         6450 g         6335 t
ORIGIN      
        1 cacaaacatt cactaagtta atttatatag tatctgccca gacacttatt tatagttatt
       61 aaaggtgcgt ccgactggtt caccggacgc accttaagta cgtttccttg tgttataaga
      121 acagaaggat cagctgtaaa acagcaatga tgattttgat ggcccgttta ataaggtatc
      181 ggcaatcagt cattcgtttt tccttaaaca aggaatgcag ccgttcagat tagccattac
      241 atctccccaa aactgaacgt gcgagttatt gagggtgcat gctgcactcc acaccagagc
      301 tttgacgaca ccactcgttt caatggggga attctgtggc atggtgtaaa gcacagcaaa
      361 atcgccaata acgaaaccaa ttttaatgta cttacgaatt ggcggtcacg ttaatcttgc
      421 catcaatatc gctttcttcg taaaggctcg agtttttatg ataaagattg caagttgctt
      481 gtaaaagata actaccctga tccataatcg ctgttgttga gggtgcgtgc tgcactaaat
      541 taaagttaca aagtaaaacc cccgttcctt accagttcgg gggttttact ttttaaagag
      601 aacggtatta tttttaactt tcaataatta ccgtggcgca agcatagtgg cgctcatcgg
      661 ccagcgtcac atgcatattt gcaacgccca gcttttccgc cagttttaat gcctcgcccc
      721 acagtcgcag ccgtggctta ccaagctcat cgttaaatac ttcaaactga ttaaacgcca
      781 gaccattgcg gatcccggtg ccaaacgctt ttgctgcggc ttcttttacg gcaaagcgtt
      841 tcgccagaaa acgcaccggc tggtggtgcg ttttccaaat ttcccattcg ttatcgctta
      901 atacgcggcg tgccaggcgc tcaccggatc gggcgatcac tgcttctatg cgagcgatct
      961 cgacgatatc cgtgcctaaa cctaatattg ccattagcca cgcgcttcca gcatcaggcg
     1021 cttcatttct gccaccgcat ctttcagtcc ggtcatcact gcacgaccaa taatggcatg
     1081 accgatattc agttcgtgca tctcagggat ggcggcaatg gcttttacgt tgtgataggt
     1141 cagaccgtgt ccggcgttaa ctttcaaacc gagactagcg gcaaaggtcg cggctttggc
     1201 gatacgcgcc aactcttgcg cctgttcggc gtcagttttg gcatcagcat agcaaccggt
     1261 gtggatctcg ataaacggtg cgccaacctc tgccgcagct ttgatctgct cttcatcggc
     1321 gtcgataaac agagaaacct gaatcccggc atccgccaga cgtttgcagg cattgcgcat
     1381 tttttcacgc tgccctgcaa cgtccaggcc gccttcggtt gttacttcct gacgcttttc
     1441 tggtaccagg cagcaaaaat gcggcttcgt ctcaaccgcg atcgccagca tctcttcggt
     1501 caccgccatc tccagattca tgcgggtatc cagcgtctga cgcaggatgc gcacatcgcg
     1561 gtcagtaatg tggcggcgat cttcacgtaa atgcacggta atgccgtccg cccccgcctg
     1621 ctcggcaata aacgcggcct gcaccggatc cgggtaagcg gtaccgcgcg cgttgcgcag
     1681 cgtagcgata tggtcaatgt tgacgcctaa cagtaattca gccatgacaa tcctcatcat
     1741 tcataatgtg ttttcaccgt tcgcttaggc ataaattgcc ggaacagttc cctgctcttt
     1801 aaaggtttac cgccaagata cggcttaagc gccatgcggg taaagcgttt cgcggcgcgc
     1861 agtgtgtctg cgtcaggaaa ttcccgtgcg tttaacgctt ttaactgcct tccggtgaac
     1921 gttttattgt cgataacgac gcttgcgata aacccttttt cttcgcgata acgatacgtc
     1981 atggtgtcat ctaccggctc gccgctaccc gcacaatggg taaaattgac gccataaccc
     2041 agatgcccga gcagtgccag ttcaaagcgg cgcagcgcgg gttctggcgt accagtgacc
     2101 cctgcaagag actgaatgca gtgcaagtaa tcgaaaaaga gttcagagaa gcgcgtctcg
     2161 tattccagta cgcgggagag aagttcgttg atgtacagac cgctgtaaag cgtgataccg
     2221 cttaatggca gcgccagcga gacagcttca gcactgcgca gcgttttgac ttcgccacgc
     2281 ccgccaaaac gtagcaagag aggggtgaaa ggctgtaatg cacctttcag ggttgagcgt
     2341 ttagagcgtg cgcctttggc aaccagacgc acgcgacccg attcctccgt gaagacgtcc
     2401 agcatcaggc tggtttcgct ccacgggcga ctatgcagga caaatgcgcg ctgccagcct
     2461 tccatcggag ttactcttaa agatcgtcaa cgtaaccgag actgcgcagt gcgcgttcgt
     2521 cgtcggccca accggatttc acttttaccc acagctcaag gtgaacaggc gcttcaaaca
     2581 tttcctgcat gtctttacgc gcttcaatcc cgatggtttt gatcttggcc cctttgttgc
     2641 caatgaccat cttcttctgc ccttcacgct caacgagaat caaaccgttg atgtcataac
     2701 caccgcgttc gttagagacg aaacgttcga tctccacggt cacggagtac ggcagttcag
     2761 cgccgaggaa acgcatcagt ttttcgcgga tgatttcaga cgccataaaa cgctgtgagc
     2821 gatcggtgat gtaatcttcc gggaagtgat gagtcgcttc aggtagatgc ttacgcacga
     2881 ttgccgcaat agtgtcaaca ttcagcccgg tttcggcaga gattggcacg atatcgagga
     2941 agttcatctg gcttgccaga aactgcaggt gcggcagcag atcggctttc tcctgcacgt
     3001 tgtccacttt gttcaccgcg aggattaccg gcgctttgcc ttcgcgcagt ttgttgagca
     3061 ccatttcgtc gtccggcgtc cagcgggtgc cttcaacgac aaaaatcacc agctcaacat
     3121 cgccaataga gctgctcgcc gctttgttca tcaggcggtt aatggcgcgt ttttcttcca
     3181 tatgcaggcc cggtgtatcg acgtagatcg cctgatacgc gccttcagta tggatcccca
     3241 caatgcggtg acgagttgtc tgcgccttgc gggaggtgat ggagattttc tgccccagca
     3301 gtttgttcaa caatgtggat ttgccaacgt tcggacgtcc gacgatggca ataaatccgc
     3361 agtaactttt atcgatgctc attccagctc cagttttttc aacgcctgtt cggcggcagc
     3421 ctgctcagcc ttacgacggc ttgaacctgt gccaaccacc ggttcactca ggccgctgac
     3481 ctggcagtgg attgtaaatt cctgatcgtg cgcttcgcca cgtacctgga ctaccagata
     3541 agtcggcagc ggcagatgac gaccctgcaa atattcttgc aagcgcgttt tcggatcttt
     3601 ttgtttatcg cctgggctaa tttcgtccag acgagtttga taccagttga ggattaattt
     3661 ctcgacggtt tgaatatcac tgtcgaggaa tacgccacca attaatgctt cgacggtgtc
     3721 ggcgagaatt gactcacgac gaaatccacc gcttttaagt tcacctggtc ctaaacgtaa
     3781 gcactcgcct aactcaaatt cgcgcgccag ttccgccagc gtattgccac ggaccagcgt
     3841 ggcgcgcatc cggctcatat cgccttcatc cacacgaggg aaacggtgat aaagcgcatt
     3901 ggcgataacg tagctcagaa tagagtcgcc taaaaattct aaacgctcgt tatgtttgct
     3961 gctggcacta cgatgagtta atgcctgctg caacagttcc tgatgattaa aagtgtagcc
     4021 cagcttccgt tgaagccgat taattacgat ggggttcatg cgataccaat aaataaatgc
     4081 gtcaacaatt cagcacacga aacagacctg atatacatgg ctctgctaac tgcttcgctg
     4141 cagtttcagt ttagcggtat atggggacca acgctgtttc gtgtgccgtg gcaacctgga
     4201 ggtgccaacc ttaaacttcg ggggaatatt ctatacacaa cgacggggga tgtcgttagc
     4261 cacgggagat ttatctcata aataattcac gttgtcgcca taacggcgac aacgtgaacg
     4321 aagatgagct attaatggat gccgccaatg cgacttaagc gcacacctgt cggccattcg
     4381 ccttcttgct tatcgaagct catccagata gccgttgccc gaccgaccag attcgcttca
     4441 ggcacaaagc cccagtaacg gctgtccgcg ctgttgtcgc ggttgtcgcc catcatgaag
     4501 tattgtcccg gaggaacaat ccaggttgcc agttgttgcc ctggctgctg gtaatacatc
     4561 cccacctgat cctgcgcaat cggcactgtc agaatgcggt gcgtcacatc acccagtgtc
     4621 tctttacgct cggaaagacg aattccattt tctttggttt cgtttttcgg cacttcaaag
     4681 aatccgctgg tcgcttcccc accattacgg cgtgagaagg tctgaacgaa atcgctcggt
     4741 tccacgtttg agtaggtgac cggcagcgcg ttttcacacg cctggccgga actgcatccc
     4801 ggttgaatcg tcagctcttt tgagaccgga tcgtaagtga ctttatcgcc cggtaaaccc
     4861 accgcgcgct tgatgtaatc aagctttgga tcttccggat atttaaagac cacgatatcg
     4921 ccgcgtttcg gatgaccggt ttcgatcagc gttttctggt agataggatc tttaatgcca
     4981 taagcaaact tctctaccag aataaaatca ccgattaaca gagtcggcat catcgaacct
     5041 gacggaatct ggaacggttc ataaataaac gaacgcacaa tcaatacgat agccagtacc
     5101 ggaaaaacag aagcaccggt ttccagccag ccaggcttcg gcgcaacctt tttcaacgtt
     5161 gctttatcca gtgagtcacc ggcagccgcc tgcgccgctg cctgacgttc ccgccgttta
     5221 ggtgcgaaaa agaatttatc cacgcaccat aaaatgcccg tcaccagtgt ggcaatcacc
     5281 agaatcaggg caaacatatt cgccatgcca actcctaagg gttatttgtt gtctttgccg
     5341 acgtgcagaa tggcgaggaa cgcttcctgc ggcagctcga cgttaccgat ctgcttcatg
     5401 cgtttcttac cttctttctg cttctgcagc agctttttct tacggctgat atcgccgcca
     5461 taacatttag ccagtacgtt tttacgcagc tgtttcacgg tggatcgcgc aataatgtgc
     5521 gtaccaatcg ctgcctgaat ggcgatatca aactgctggc gtgggatcag atctttcatc
     5581 ttctccacca actcgcgacc gcggttttgc gaattatcac ggtgggtgat caacgccagc
     5641 gcatcaacac gttcaccgtt gattaatacg tctacacgta ccatgtcgga cgcctggaag
     5701 cgtttgaagt tgtaatccaa agacgcatag ccacgcgagg ttgatttcag tcggtcgaag
     5761 aaatcaagca ccacttccgc catcgggatc tcgtacgtca gcgccacctg attaccgtgg
     5821 taaaccatat tggtctgcac gccgcgtttt tctacgcaca gcgtaataac gttgccgaga
     5881 tatgcctgcg gcagcagcat gtgacactct gcaatcggct cgcgcagttc gtaaatgtta
     5941 tttaccgcag gcagcttgga tgggctgtcg acgtagataa cttcccttga cgtggtttca
     6001 acttcataca ctacggtcgg cgcagtggtg atcagatcca gatcgtattc acgttccaga
     6061 cgctcctgga tgatctccat gtgcagcagg ccaaggaagc cgcagcggaa accaaagccc
     6121 agcgcgctgg agctttccgg ctcatagaac agtgaggcat cgttcaggct gagtttaccc
     6181 agcgcgtcac ggaaggcttc atagtcgtcg gaacttaccg ggaacagacc ggcgtatacc
     6241 tgcggtttga ctttcttaaa gccaggcagc gccttttctg ccggattacg cgccagcgtt
     6301 aaggtatcgc cgactggagc gccgtggata tctttaatcg cacatacgag ccagcctact
     6361 tcgccacatt tcagttcagt gcggtcaacc tgtttcggcg tgaagatgcc cagacggtcg
     6421 gcgttatagg tctgcccggt actcatgact ttcactttgt cgcccttacg cagggtgccg
     6481 tttttaatac ggataagtga aacaacgccc aggtagttgt cgaaccatga gtcgataatt
     6541 agtgcctgca acgggccttc cggatcgcct tccggcggcg gaatgtcgcg caccagacgt
     6601 tcgagaacgt cctgcacgcc aacgccggtt ttcgctgaac agcgcaccgc gtcagtggcg
     6661 tcgatgccga cgatatcttc aatttcttcc gccacgcgtt caggatcggc tgccggcagg
     6721 tcaatcttgt tcagtaccgg cactacttcg agatccatct ccatggcggt gtagcagttc
     6781 gccagagttt gcgcttccac gccctgcccg gcgtcgacca ccagcaatgc gccttcgcag
     6841 gccgccagcg aacgggaaac ttcataggag aagtcaacgt ggcctggagt gtcgataaag
     6901 ttaagctgat aggtttcgcc gtcagacgct ttgtagtcca gcgtcacgct ttgcgctttg
     6961 atggtaatgc cacgctcacg ctcaagatcc atggaatcga gaacctgcgc ctccatttca
     7021 cggtcagaca ggccaccgca gatctggata atacggtcag acagcgtcga tttaccgtgg
     7081 tcaatgtgag ctatgatcga aaagttacgt atattcttca taaagtatga ttattgtgcc
     7141 ttaatgcccg gttaaccagg cttttagaag tcgctgttct gagcttaacg tctgtattaa
     7201 tagaaacgcc gcattctaca ctacaacatt gaggcgagga aatgttcata ccgtatggat
     7261 tgtggtatct ggaaacgtcc tcgcatttgt tatgcaaaat gcaacaaagc cagtgaaatc
     7321 actggctcgc gtcttccgaa gatgtttcaa atcgcacaag gccaggcggc aatgccacgc
     7381 ttaagatgat cggttgccat tctgcccggg cagcaaactt acgcgagtag ccacgcgcaa
     7441 tcaggaaccc gccaataccg ccgaggaccg caccacataa tgctgcgacg tcagaagcaa
     7501 agagtagctg aaatagcgaa gcgatgagga ataatcccac cagcggcgac atataaacca
     7561 gtaatgcgga gctaagcagg ctgccttcgg cgatccccaa ttccactttt tgccccggca
     7621 ctaacggttc atcacagggt acgacaatgg tatgcgtggt ttgcgggcca agtttattca
     7681 acacgcggct accgcaaccg gcgcgtgaag cgcagctgct gcatgaggct ttaacatcac
     7741 aactgaccag cgcctgcccg ttttgccagg agacgacggt agcccactct ttgatcattg
     7801 cgctgccccg aacttaatat tctcggcaat gcgtttcgcc gtttgcggcg gcagttcacc
     7861 gacaatggtg atttcggcgt tatcacgtac gcttgtactg acggttctgc gtccggtgcg
     7921 caacatctga tcggtgctcg atggcgtagc gcggttaacg tttaccgaga agctgaataa
     7981 tccgtcggaa tagagacgtg attcgatagg catgttgtcc atcgtcggta gcggacgtcg
     8041 actactggaa acttcgctaa aaccctgtgg caaccaggtt ggcgtccagc tgaatttagc
     8101 tttttcacct acaggaacag aaagcaacgg cggcaaattt gccttcgcca gcgtctgcat
     8161 actgctgctg atatcctgat tgacgttaaa agcaatcacg cgaaattgtt ccagcgtttc
     8221 accatcgcga tcaagaagat caacccgcat cggtaatttc gattcggtgt ccatccacac
     8281 gatgtagctg tagcgtgtgc catcacgagc aaccacgcga ataacttcac aaagacgatc
     8341 agcaatacgc gtgcgcccga cggagataaa gtcgtagtaa ggagaaaggc gtttgaaatc
     8401 ggtatagatg agcgatggca gagaatcaac aatgtaatcg ccattaagag taaacggttc
     8461 aaggcccggt tcaaaatagc tgatttcatt gccgcgctgt accacttccc ggcgcgggcc
     8521 atccatttgc aacaattgtg caagaggacg gttatcgagg cgtgcatgtc gataacgcag
     8581 agactcaaca ccctgtttat tgatgctgat gaatgacagc tcgtaattca gtgactgact
     8641 ggccaggttc atctgctgta ataacgcccc ggacgcggga gtggccgagg cgttagcaga
     8701 gaataacagg ctacctgtca ctaatgacat ggcaaaccaa agttgcttca ttactgcgat
     8761 tgcgttccta aagtttgaat tcctggcacc tgtacagcgg cttgctgggt ttgcgcctgc
     8821 tcaaactgaa gctgttcaga gtggagtcgg cgttgcagtt cgtaatcctg caacattgca
     8881 ttaatgcgac gacgctgctc ctgtacctgc tgctgttgac cattatttgc ggtcgcttca
     8941 gaaggtactc ccaggctaac cgggctggct ttacccatca tcggcagtgt attaaatacc
     9001 ggtgtttcgg gctgctggga cgtttcagat tgtccattat agtgctggac gccaacgata
     9061 actgcaagcg atacgcatgc ggctacgccc atttgggtaa gctgtgccgc ccacggacgt
     9121 actttctgcc agaatggcat tttctgccat tgatgcggcg caggctgggc ttccgggatc
     9181 aatgtcgccg gttgacgtac tggctcttct tcaatggcgg ccatcacgcg tgaagagata
     9241 tcgaaatgga gcacctcggg agtatcaccc cgcattgagt cacggattaa gtgatagctt
     9301 tcccaggttt tctgcatttc tgggttatga gccagttcgt taagcagctc actatccagc
     9361 gtttcgccat ccattaaagc ggaaagttgt tctttctgca tgcctaatac ccttatccag
     9421 tatcccgcta tcgtcaacgc ctgataagcg gttgaacttt gttatcaata gcttccctcg
     9481 ctcggaagat acgtgaacgc accgtaccta ccggacaatc catgatagcg gctatctctt
     9541 catagctcag gccatccagc tcccgcaagg ttattgccat gcgtaaatct tccgggaggg
     9601 actcaatagt tcggaaaact atctgtctca gttcttctga caacattaag ttctcagggt
     9661 tcgaaatttc tttcaacgcg ccgccacttt cgaagttttc agcttcaatg gcatccacat
     9721 cactggaagg tggacgacgc ccctgagcaa ccaggtaatt tttcgctgta tttacagcaa
     9781 tccgatacag ccatgtataa aaagcgctat ctccccggaa cgaatccagc gcacgatagg
     9841 ctttaataaa agcttcttgt accacatcgg gaacatcacc cgacggcaca tagcgggaaa
     9901 ccagactcgc cactttatgc tgatagcgca ctaccagtaa gttaaaggct ttctgatctc
     9961 ccttctggac ccgttcaacc aggacctggt ccgttaactg ctcgctcatc cgaggtaaag
    10021 tctccccaaa ccaaatttcc acgcgctatc gaaacgccac tccattagct gcaatttgag
    10081 caagcaaagg gttagagtgt ctcgtttttg taaagttccg taacgcatct gtttttgttt
    10141 gtcatgctgt agacggatca ttatctatca ttataagtct acagaatctg aacatcgcat
    10201 tatctgtgta gaaatgccca tttaactgcc tgaagagtaa cccaacggcc tttttatttc
    10261 accacctaat cctccaccag ccagtaactt ctctttttct cgccgccctg cgtcagcgtg
    10321 tttagccact gtaacaaata ttaaaatagc aggtgtttat tcgcacaaca tgatgctatg
    10381 ctgaccaaac cgtgtttagt aaattaaaca aagaaaatga atactctccc tgaacattca
    10441 tgtgacgtgt tgattatcgg tagcggcgca gccgggcttt cactggcgct acgcctggct
    10501 gaccagcatc aggtcatcgt tctaagtaaa ggcccggtaa cggaaggttc aacattttat
    10561 gcccagggcg gtattgccgc cgtgtttgat gaaactgaca gcattgactc gcatgtggaa
    10621 gacacattga ttgccggggc tggtatttgc gatcgccatg cagttgaatt tgtcgccagc
    10681 aatgcacgat cctgtgtgca atggctaatc gaccaggggg tgttatttga tacccacatt
    10741 cagccgaatg gcgaagaaag ctaccatctg acccgtgaag gtggacatag tcaccgtcgt
    10801 attcttcatg ccgccgacgc aactggtaga gaagtagaaa ccacgctggt gagcaaggcg
    10861 ctgaaccatc cgaatattcg cgtgctggag cgcaacaacg cggttgatct gattatttct
    10921 gacaaaattg gcctgccggg cacgcgacgg gttgttggcg cgtgggtatg gaaccgaaat
    10981 aaagaaacag tggaaacctg ccacgcaaaa gcggtggtac tggcaaccgg cggtgcgtcg
    11041 aaggtttatc agtacaccac caatccggat atttcttctg gcgatggcat tgctatggcg
    11101 tggcgcgcag gctgccgggt tgcaaatctc gaatttaatc agttccaccc taccgcgctg
    11161 tatcacccac aggcacgcaa tttcctgtta acggaagcac tgcgcggcga aggcgcttat
    11221 ctcaagcgcc cggatggcac gcgttttatg cccgattttg atgtgcacgg cgaactggcc
    11281 ccgcgcgata ttgtcgcccg cgccattgac catgaaatga aacgcctcgg cgcggattgt
    11341 atgttccttg atatcagcca taagcccgcc gattttattc gccagcattt cccgatgatt
    11401 tatgaaaaat tgctcgggct ggggattgat ctcacacaag aaccggtacc gattgtgcct
    11461 gctgcacatt atacctgcgg tggtgtaatg gttgatgatc atgggcgtac ggacgtcgag
    11521 ggtttgtatg ccattggcga ggtgagttat accggcttac acggcgctaa ccgcatggcc
    11581 tcgaattcat tgctggagtg tctggtctat ggctggtcgg cggcagaaga catcaccaga
    11641 cgtatgcctt atgcccacgg cgtcagtacg ttaccaccgt gggatgaaag ccgcgttgag
    11701 aaccctgacg aacgggtagt aattcagcat aactggcacg agctacgtct gtttatgtgg
    11761 gattacgttg gcattgtgcg cacaacgaag cgcctggaac gtgccctgcg gcggataacc
    11821 atgctccaac aagaaataga cgaatattac gcccatttcc gcgtctcaaa taatttgctg
    11881 gagctgcgta atctggtaca ggttgccgag ttgattgttc gctgtgcgat gatgcgcaag
    11941 gagagtcgtg gcctgcattt tacgctggat tacccggaac tgctcacgca ttccggtcca
    12001 tcgatccttt ctcccggcaa tcattacata aacagataaa aagcctgggt tagcgccgta
    12061 tacgcttcgg aatagttctg gtctggccca cgaatgacta agcgatcgct aaagcattct
    12121 cccgcctgcg gggagaatgc cagcagcacc cgatgcggca gtcgcgcttc gttttccgcc
    12181 acatccgtcc gcaaacgtaa atgccagccc atacttaatg ccagttcagt aaaaccatta
    12241 ccaatctgct ctggcagcac cacgcagaaa aatccctctt cggtaatgca ctccgccgca
    12301 caggtcagca acgatgggtg atcaagcgta gtagtatagc gagcctgttc ccgttgaggt
    12361 gtcgagcact ctactccctg ctgatagtaa ggtgggttac tgatgattaa atcgaagcgt
    12421 actgtctgct gtgtgatcca ctgctgaata tccgccgtat ggacgttaat ccgctctgcc
    12481 cacggggact ggttgatatt ttcctgcgcc tgcgcggcag cttcactttc cagttcaact
    12541 gcatcaatca tcacgctgtc atcggttcgc tgcgccagca ttaatgccag caacccgctc
    12601 cccgcgccga tatcaaggca acgttttacc ccagccaccg gtgcccatgc gcccaataaa
    12661 ataccatccg ttcccacttt catcgcacag cgatcgtgag caacaaaaaa ctgtttaaaa
    12721 gtaaatccat tacgacgaag cacggatgta gactgtgaca tgaaaataaa accttgcagg
    12781 aaaaacggcg atagcaccgg gtgagaacaa tacccgagaa gcgatatcca tacaaacaga
    12841 tgaagattgc agccgtaacg tctataatca gcgccccaca cagaggtaga acatgactgt
    12901 aacgactttt tccgaacttg aactcgacga aagcctgctg gaagccctcc aggataaagg
    12961 tttcactcgc ccgaccgcca ttcaggctgc cgccattccg cctgcgctcg atggccgtga
    13021 tgtactcggt tctgcgccga caggcaccgg taaaacggcg gcgtatctgc tgccagcgtt
    13081 gcagcacctg ctcgatttcc cgcgtaagaa atccggtccg ccgcgtattt tgatcctcac
    13141 cccaactcgc gagctggcga tgcaggtggc cgatcatgcc cgcgaactgg cgaaacatac
    13201 gcatctggat atcgccacca tcaccggcgg cgtagcctat atgaaccacg cggaagtgtt
    13261 cagcgaaaat caggacatcg tggtcgccac gaccggacgt ctgctgcaat acataaaaga
    13321 agagaacttc gattgccgcg cggttgaaac gctgatcctc gacgaagcag accgtatgct
    13381 ggatatgggc ttcgcccagg atattgaaca tattgctggc gaaacgcgct ggcgtaaaca
    13441 gaccctgctc ttttcggcaa cgctggaagg cgatgcgatt caagactttg ccgagcgtct
    13501 gctggaagat ccggtggaag tttctgctaa tccttccact cgtgagcgca aaaaaattca
    13561 tcagtggtat taccgcgccg atgacctgga acataaaacc gcattgctgg tgcatctgtt
    13621 aaaacagccg gaagcgaccc gctcaattgt gtttgtgcgt aagcgtgagc gtgtgcatga
    13681 gctggcaaac tggctgcgcg aagcgggcat caacaactgc tatctcgaag gtgagatggt
    13741 acaaggcaag cgtaacgaag cgatcaagcg tttgaccgaa ggtcgcgtaa acgtactggt
    13801 tgcaaccgat gttgctgcgc gcggtatcga cattcctgac gtcagccacg tctttaactt
    13861 cgatatgccg cgcagtggcg atacttattt gcaccgtatc ggacgtaccg cgcgcgccgg
    13921 tcgtaaaggc accgcaattt cgctggtaga agcccatgac catctgctgc tgggcaaagt
    13981 tggtcgctat attgaagagc caattaaagc tcgcgtaatt gatgagttac gcccgaaaac
    14041 gcgtgcacca agcgagaagc agaccggcaa gccatcgaag aaagtactgg caaaacgcgc
    14101 cgagaagaaa aaagctaaag agaaagagaa gccgcgggtg aaaaaacgcc atcgcgatac
    14161 caaaaatatt ggtaagcgcc gtaaaccaag cggaacgggc gtgccaccgc aaacgacaga
    14221 agagtaatct caatgccggg tttaagcccg gcattatttt tatcccggca aaaaacaatc
    14281 ttcaacacac tgaataaaag tgtgcatcgc cgggctaacc gcttttccgg catggtgagc
    14341 gcacattgcc gtaatggtct gcgagtgttc gccaaaaggc aattcaatta attctccgct
    14401 ctcaagctct ttttccaccg caaagcgggg caagtaactg acaccgatat tcgccgcgac
    14461 acaacgcttg atactttcaa tgcttaacag ctcaatggtg ttttccaccg tgatccgtcg
    14521 ctggcgcagc gtgctctcaa atatctgccg gaagacacat tgcggttcgt tgataataaa
    14581 gctacaggcg ttatgtcttc ccggctcagt aaaatcgaca tctgcaattt gtggtgaagc
    14641 caccagcacc agcgattgtt cacccaactc tcgtcggttc agggcatcat cattccctac
    14701 gcgataaaag acgccgacat cggcctcatc attcagtagc gcatcacgaa tcacgtaaca
    14761 gttcagcgac tgcaacgata aacgcacttt tggtgcgcgt tgccgaaaac gctgcaacac
    14821 ctgcggcatt cggtaagaga gtaacgtttc acccgaaaca acgcgtagtt ccccgtccgg
    14881 atccgactcc tttttggcag cctcacgaag ggtatccatc actctggtaa gttcatagat
    14941 gtgcggcagc agctttttcc cttcgcgggt aaggcacatt cgtcgaccaa ttttctcaaa
    15001 taactgtact gagaactcct gctcaagctg ctgaatatga aaagtcactg tcgattgtgt
    15061 gcagcacaat ttttgcgaag ctcgcaaaaa ggaaccctct tccaccacgg ttttaagcgt
    15121 aataaaacgg cgcagatcca taaccccaaa cctatcgaaa atatcgaatt tagaatgtaa
    15181 aaacattcat ttttttaaat attccgtgtc gggtactgtc taccaaaaca gaggagataa
    15241 caagtgacac cgacgctttt aagtgctttt tggacctaca ccctgattac cgccctgacg
    15301 ccaggaccga acaatattct cgctcttagc tcagccacta cgcacggctt tcgtcagagt
    15361 acccgcgtac tggcagggat gagtctggga tttttgattg tcatgctgct gtgtgcaggc
    15421 atttcgtatt cactggcagt gattgatccg gcagcggtac accttttgag ttgggcagga
    15481 gcggcataca ttgtctggct ggcatggaaa atcgctacca gtccaacaaa ggaagacggg
    15541 cttcagacaa aaccgatcag tttttgggcc agctttgctt tacagtttgt gaacgtcaaa
    15601 atcattttgt acggcgttac ggcactttcg acgtttgtcc taccgcaaac gcaggcgtta
    15661 agctgggtag ttggcgtcag cgttttgctg gcgatgattg ggacgtttgg taatgtgtgc
    15721 tgggcgctgg cggggcatct gtttcagcga ttgtttcgcc agtatggtcg ccagttaaat
    15781 atcgtgctcg cgctgctgct gatctattgc gcggtacgca ttttctatta attaaaaaag
    15841 cggaagaagt cgccctcttc cgcttagtaa cttgctactt aagccttaca ggctttcagt
    15901 aaaggtacga gcaataacgt cgcgctgctg ttccggagtc agagagttaa agcgaactgc
    15961 atatccggat acacggatgg tcagctgcgg atatttttcc ggatgcttaa ctgcatcttc
    16021 cagagtttca cgacgcagaa cgttaacgtt caggtgttgg ccaccttcaa cgcgaacttc
    16081 tggtttcact tctactggaa cttcacggta ctcaatatca cccagtttgc ttactgcaac
    16141 cacttcgtct tctgcaaaac ctgctttcgc aacgatgcaa cgcgcttcgc ctttttcgct
    16201 gtccagcagc cagaaagagt tcagcagatc gtcgttagcg gctttagtaa tctggatacc
    16261 tgtaatcatg tgatgcctcc ccggcaaaat tatttgattt gttcagcctg tcgcggccaa
    16321 ttggtaaaac cattgttgct tgagtgtata tatactcctc aaacaccctt gaatctttga
    16381 tttaaatcaa taaaaaccac acatcaagtg tggtcgcaaa tggattttat tgttttacat
    16441 caacttatgc gggtgtgaaa ttttaccaat ttacattttt ttgcactcgt ttaagtctaa
    16501 aaaatgagca tgattttgtt ctgtagaaag aagcagttaa gctaggcgga ttgaagattc
    16561 gcaggagagc gagatggcta acgaattaac ctggcatgac gtgctggctg aagagaagca
    16621 gcaaccctat tttcttaata cccttcagac cgtcgccagc gagcggcagt ccggcgtcac
    16681 tatctatcca ccacaaaaag atgtctttaa cgcgttccgc tttaccgagt tgggtgacgt
    16741 taaagtggtg attctcggcc aggatcctta tcacggaccg ggacaggcgc atgggctggc
    16801 attttccgtt cgccccggaa tcgccactcc tccgtcactg ctgaatatgt ataaagagct
    16861 ggaaaatact attccgggct tcacccgccc taatcatggt tatcttgaaa gctgggcgcg
    16921 tcagggcgtt ctgctactca atactgtgtt gacggtacgc gcaggtcagg cgcattccca
    16981 cgccagcctc ggctgggaaa ccttcaccga taaagtaatc agcctgatta accagcatcg
    17041 cgaaggcgtg gtgtttttgt tgtggggatc gcatgcgcaa aagaaagggg cgattataga
    17101 taagcaacgc catcacgtac tgaaagcacc gcatccgtca ccgctttctg cgcatcgtgg
    17161 attctttggc tgtaaccatt ttgtgctggc aaatcagtgg ctggaacaac atggcgagac
    17221 gccgattgac tggatgccag tattaccggc agagagtgag taaatttgcg ggaaaatgcc
    17281 ggatggcaga gttgccaccc ggctgattta tcaggcttta ttctgacgcc accattcacc
    17341 aagcaaaacg cctgttgcaa cagagatatt cagcccggca acgttgcccg taccgtcaat
    17401 cttcacgcgc agatcgttcg gatcgcgtgc ggcatccggt aacccttcat attcctgacc
    17461 cagcaccagt accattttcg ctggcaggct ggttttgaac agcggtttac cctgctcgct
    17521 ggaagttgtc actacggtgt aacccgcctg acggaaatca tccagcacgt taacaatgtt
    17581 gtcgccagta atcggctgaa cgtgttctgc gccgccttct gcggtacgga tagccgcacc
    17641 cgactccagc agtgccgcat cctgcaccac aacgcctttc acgccgaagt gcgcgcagct
    17701 gcgcatcatg ccacccaggt tatgcgggtt agattcgttt tccagtgcca gaacgcaatc
    17761 ctgcgcacct gcctggctta cccactgctg cacggttgtg ccattacgct ttttgatcag
    17821 gaagcaaacg ccgccgtgat gttccgtgcc tgacgctttt gtcaattccg cttcatccac
    17881 cacatggtac gctttgcggt ttgccgccat ccagcgcaag gcttctttaa aacgtggcgt
    17941 tacgctctgg ataaaccagg cgcgaacaat cgcttccgga cggctctgga acagtgcctg
    18001 acatgcattt tcgccgtaga cgcgggtttc ttccgcacgc tgacgacgca acacttccgg
    18061 atcaataaaa cttttaccac tgataccacc gtgatcggcc ttttccggcg tctcatcacc
    18121 cggcgcgcgg gaaaccgtgc gccacggcga ctcttcccac ttgcggtcac ggggctgact
    18181 ctgtttgtca tcgcgggcgg ggcgacggcc accgtcagca cgagattttc ctggacgccc
    18241 gccccctttc ccggtacgcg ggttgtgggt acgtttatca gaatcatcat cactgcggac
    18301 atacatcact ttgaccttgc cgcttttacc tttcatttca tcgttcatgc ttttctccac
    18361 cagcgctgcg cgaagcgcgc agattacccg aagtccgcgc ggttcgccat gatttcgtac
    18421 caaagcctgc gactatcata cctattgaat aaaacagatt gttgtctgga acaatgtccc
    18481 cgataatatg taacatatta gaaacatacc ggcgtcgttg ccgataagtc tccttactca
    18541 tcccgaggtt agttatgaat accgtttgta cccattgtca ggccatcaat cgcattcccg
    18601 acgatcggat cgaagatgcg gcaaaatgcg gacgctgcgg tcacgacttg tttgacggag
    18661 aggtgattaa tgcgaccggt gaaacgctcg acaaattgct gaaagatgat ctaccagtgg
    18721 tgatcgactt ctgggcaccg tggtgcggcc cctgccgtaa tttcgcacca atttttgaag
    18781 atgtcgcgca agagcgtagc ggtaaagtgc gctttgtgaa agtgaatacc gaagctgaac
    18841 gtgaattaag cagccgcttt ggtattcgca gtattccgac gatcatgatt ttcaaaaacg
    18901 gtcaggttgt cgacatgctt aatggcgcag tgccgaaagc gccgttcgac agctggttga
    18961 acgaatctct ttaatcttac cggggcgcat cttgtgcccc gttttctcct ctgcgacaat
    19021 ggcgtttttt cgacgctctc ttatgaccga aaacgctgtt ctccagttac gcgccgaacg
    19081 tattgcgcgc gcaacacgtc cttttcttgc ccgcggtaat cgcgttcgtc gctgccaacg
    19141 ctgtcttttg ccagagaaat tatgtctctg ttcaacaatc acgccagcgg aagcaaaaag
    19201 tcgcttctgt ttgctgatgt tcgacaccga gccaatgaag cccagtaata ccgggcgtct
    19261 gattgctgat attttgcctg ataccgttgc gtttcaatgg tcgcgtaccg aaccctcgca
    19321 ggatttgctg gatctggtac aaaacccgga ttatcagcca atggtggtct ttcccgcctc
    19381 gtatgctgat gagcaacggg aagtgatctt cacaccacct gccggtaagc caccgctgtt
    19441 tatcatgctc gatggtacct ggccggaagc tcgcaagatg tttcgtaaaa gtccgtatct
    19501 ggataatctt cccgtcattt ccgtcgatct ttcccggctt tctgcctatc gcctgcgtga
    19561 agcccaggct gaaggccaat attgtactgc cgaggtagcc atcgcactgt tagatatggc
    19621 cggcgatacc ggggcggcag cagggttagg cgagcatttt acccgcttta aaacacgcta
    19681 tctggcagga aaaacgcaac atctgggtag catcacagca gaacagttag aaagcgttta
    19741 aaatcattcg gtcacttctg cgggagaccg gtatgagtca gcgaggactg gaagcactac
    19801 tgcgaccaaa atcgatagcg gtaattggcg cgtcgatgaa acccaatcgc gcaggttacc
    19861 tgatgatgcg taacctgctg gcgggaggct ttaacggacc ggtactcccg gtgacgccag
    19921 cctggaaagc ggtgttgggt gtgttggcct ggccggatat tgccagcttg ccctttacac
    19981 ccgaccttgc ggttttatgt accaatgcca gccgtaatct tgctcttctg gaagagctcg
    20041 gcgagaaagg ctgtaaaacc tgcattattc tttccgcccc ggcatcgcaa cacgaagatc
    20101 tccgcgcctg cgccctgcgc cataacatgc gcctgcttgg accaaacagt ctgggtttac
    20161 tggctccctg gcaaggtctg aatgccagct tttcgccggt acctattaaa cgcggcaagc
    20221 tggcgtttat ttcacaatcg gctgccgtct ccaacaccat cctcgactgg gcgcaacagc
    20281 gtgagatggg cttttcctac tttattgcgc tcggcgacag cctggatatc gacgttgatg
    20341 aattgcttga ctatctggca cgcgacagta aaaccagcgc catcctgctc tatctcgaac
    20401 agttaagcga cgcgcgacgc tttgtttcgg cggcccgtag tgcctcgcgt aataaaccga
    20461 ttctggtgat taaaagcgga cgtagcccgg cggcacagcg actgctcaac acgacggcag
    20521 gaatggaccc ggcatgggat gcggctattc agcgtgccgg tttgttgcgg gtacaggaca
    20581 cccacgaact gttttcggcg gtagaaaccc ttagccatat gcgcccgctg cgtggcgacc
    20641 ggttgatgat tatcagcaac ggtgctgcgc ctgccgcgct ggcgctggat gccttatggt
    20701 cacgcaatgg caagctggca acactaagcg aagaaacctg ccagaaactg cgcgatgcac
    20761 tgccaggaca tgtggcaatc tctaacccgc tcgatctgcg cgatgacgcc agcagcgagc
    20821 attatgtcaa aacgctggac attctgctcc atagccagga ttttgatgcg ctgatggtta
    20881 ttcattcgcc cagcgccgct gctcccgcaa cagaaagtgc gcaagcatta attgaagcgg
    20941 taaagcatca tccccgcagc aagtatgttt ctctgctgac gaactggtgc ggcgagcact
    21001 cctcgcaaga ggcacgacgt ttattcagcg aagccgggct gccgacctac cgtaccccgg
    21061 aaggaaccat cactgctttt atgcatatgg tggagtaccg gcgtaatcag aagcaactac
    21121 gcgaaacgcc ggcgttgccc agcaatctga cttccaatac cgcaaaagcg catcttctat
    21181 tgcaacaggc gattgccgaa ggggctacgt cgctcgatac ccatgaagtt cagcccatcc
    21241 tgcaagcgta tggcatgaac acgctcccta cctggattgc cagtgatagc accgaagcgg
    21301 tgcatattgc cgaacagatt ggttatccgg tggcgctgaa attgcgttcg ccggatattc
    21361 cacataaatc ggaagttcag ggcgtcatgc tgtacctgcg tacagccaat gaagtccagc
    21421 aagcggcaaa cgctattttc gatcgcgtaa aaatgacctg gccgcaggcg cgggtccacg
    21481 gcctgttggt gcaaagtatg gctaaccgtg ctggcgctca ggagttgcgg gttgtggttg
    21541 agcacgatcc ggtttttggg ccgttgatca tgctgggtga aggcggtgtg gagtggcgtc
    21601 ctgaagatca agccgtcgtc gcactgccgc cgctgaatat gaacctggcc cgctatctgg
    21661 ttattcaggg gatcaaaagt aaaaagattc gtgcgcgcag tgcgctacgc ccattggatg
    21721 ttgcaggctt gagccagctt ctggtgcagg tttccaactt gattgtcgat tgcccggaaa
    21781 ttcagcgtct ggatattcat cctttgctgg cttctggcag tgaatttacc gcgctggatg
    21841 tcacgctgga tatcgcgccg tttgaaggcg ataacgagag tcggctggca gtgcgccctt
    21901 atccgcatca gctggaagaa tgggtagaat tgaaaaacgg tgaacgctgc ttgttccgcc
    21961 cgattttgcc agaagatgag ccacaacttc agcaattcat ttcgcgagtc accaaagaag
    22021 atctttatta ccgctacttt agcgagatca acgaatttac ccatgaagat ttagccaaca
    22081 tgacgcagat cgactacgat cgggaaatgg cgtttgtagc ggtacgacgt attgatcaaa
    22141 cggaagagat cctcggcgtc acgcgtgcga tctccgaccc tgataacatc gatgccgaat
    22201 ttgccgtgct ggttcgctcg gatctcaaag ggttaggctt aggtcgacgc ttaatggaaa
    22261 agttgattac ctatacgcga gatcacggac tacaacgtct gaatggtatt acgatgccaa
    22321 acaatcgtgg catggtggcg ctggcccgca agctcgggtt taacgttgat atccagctcg
    22381 aagaggggat cgttgggctt acgctaaatc ttgcccagcg cgaggaatca tgagtaaggt
    22441 actggaaatg ttgaccactt aatcgggact ggtgttatta ttgcccgctt atgtcgtctg
    22501 cattgcacag aggacccttc aatgaacaga gaagaaatgc actgtgatgt tgtcaaaatt
    22561 taagcgtaat aaacatcaac aacaccttgc ccaactaccc aagatttctc aatcagttga
    22621 tgatgtcgat ttcttttacg ctcccgccga cttccgggag acgctgctgg aaaaaatagc
    22681 cagcgcgaag cagcgcattt gcattgtcgc cctgtatctc gaacaggatg acggtggcaa
    22741 aggcattctg aacgcgttgt atgaagctaa aaggcagcgt ccggaactgg atgtgcgggt
    22801 gctggtcgac tggcatcgtg cacaacgtgg acgcattggc gctgcggcat ctaacactaa
    22861 cgctgactgg tactgccgca tggcgcagga aaatccgggc gtagatgttc cagtttatgg
    22921 cgttccaatc aatacccgtg aagcccttgg cgttctgcac tttaaaggct ttatcatcga
    22981 cgatagcgta ctttatagcg gtgccagcct gaacgatgtt tacctgcatc agcacgataa
    23041 atatcgctac gaccgttatc accttatccg taaccgtaag atgtcagaca ttatgtttga
    23101 atgggttaca cagaatatta tgaatggccg cggcgttaat cgtctggatg atgttaatcg
    23161 gccaaaaagc ccggaaatca agaacgatat tcgtctgttc cgccaggagc tgcgtgatgc
    23221 cgcttatcat ttccagggcg atgccgacaa cgatcagctt tccgtaacgc cgctagtggg
    23281 gctggggaaa tcgagtctgt tgaacaagac cattttccat cttatgcctt gtgccgagca
    23341 gaaactaacc atctgtacgc catacttcaa cctgccagca atccttgtgc gcaatattat
    23401 ccagttgctg cgcgaaggga aaaaggtcga aattattgtt ggtgataaaa ccgcgaacga
    23461 cttctacatt ccggaagatg aacctttcaa gataattggc gcattgcctt atctctatga
    23521 gatcaatctg cgtcgtttcc tgagccgttt gcagtattac gtcaatactg accagctagt
    23581 ggttcggtta tggaaagatg acgacaacac ctatcaccta aaagggatgt gggttgatga
    23641 taagtggatg ttgatcaccg gtaataacct gaacccgcgc gcctggcgtc tggatctgga
    23701 aaacgccatt ttgatccacg atccacaact ggagcttgcg ccgcagcgag agaaagaact
    23761 ggagctgatc cgcgagcata ccaccatcgt taagcactat cgcgatctgc aaagtattgc
    23821 cgattatccg gtgaaggttc gtaaactcat ccgccgtttg cgccgtatcc gcatcgaccg
    23881 attaattagc cgcatcctgt aatcacaacc ccgtcctgta cggggtttgt tttttggagg
    23941 ccacgttttg cgtattcttt ttgtctgttc gctattgtta ctttctggat gcagccatat
    24001 ggctaacgat agctggagcg ggcaggataa agctcaacac tttatcgcct cggcgatgct
    24061 ttccgccgcc ggaaatgaat attcacagca tcaggggatg agccgggatc gcagtgccat
    24121 gtttggattg atgttctctg tcagtttggg ggcgtcaaaa gagctttggg atagccgccc
    24181 cgaagggagc ggctggagct ggaaggattt ggcctgggat gtcgccggtg caagcaccgg
    24241 ctataccgtc tggcaactga cccgtcacta aagacgcatc cccttccctt tgcgatgtag
    24301 catcaaagaa accagaaacg ccaccacggc catcaaggtc acataccaga agaaggctgt
    24361 ttccattcct attgatttca gcgacaacgc tacgtactcc gccgaaccac caaatatagc
    24421 attagcgacc gcatatgaca gaccaacgcc taatgcgcga acctgtgccg ggaacatctc
    24481 agccttcagt attccactga ttgatgtata aaaactcact atcagcaggg cacacatcac
    24541 cagaccaaaa gcggcataag gcgaggaaac gttttgcaat gctgagagaa taggaacggt
    24601 aaaaatggct gccagtgaac cgaaacataa cattgaggta cggcgaccaa tcttatccga
    24661 cagcgcgcca atgagtggtt gaataagcat gaatacaaac aatgcggcag tcataatgcc
    24721 actcgccacg ttggcatgca ttcccgcagt atttaccaga tacttctgca tataagtggt
    24781 gaaggtatag aaacaaaggg agcccgcagc ggtaaaaccg agaaccatga tgaatgcacg
    24841 gcgattgcgc cataatcctt tcagagatcc agcttctttt aaagcgcgcg tttcttgttg
    24901 cgaagtttca tctaactgac gacgtaacca caacgccaca acagctaaca cagctcctaa
    24961 cgcgaaagga atacgccatc cccactctct gagtgcagcg tcttccatgg tgtgttgtaa
    25021 aaccacgaca accagtaggg ctagcagttg tccgccgatc aacgtcacat actgaaatga
    25081 tgcgtaaaaa cctttgcgcc cttcaacggc aacttcactc atataggtgg cgctggtgcc
    25141 atattctccg ccaacggata atccctgaaa taaacgcgcg agaagcaata atgccggagc
    25201 ccacgtaccg attgtttcat aaccaggcaa acaggcgatt accagtgagc cgaaacacat
    25261 catacacacc gatagcagca tcgatttttt gcgaccatgt ttatcggcta tgcggccaaa
    25321 tagccaaccg cctattgggc gcatgaggaa tcccgcagca aaaacacctg ctgtttgtag
    25381 tagttgagtc gtcgtgttcc cggaagggaa gaagatgtgg gcaaagtaga gtgaacagaa
    25441 cgagtagaca tagaaatcga accactcgac cagattacct gaagaggccc ccacaatcgc
    25501 ccaaatgcgg cgacgtgtat cactacttgt cagtttgctg tctgccgtta cagtactttc
    25561 agccatgcca ttatgtctcc tgccgtaatc cgatgctttt gtcggtcgct tttgtttatt
    25621 tcttttggta aagaaaatgt tatacatttg ttgcgtgtca ttatgcaacc ttaaccatga
    25681 atttagttag caggaaaatg gttattgagg agcttaagga taaatttctg gtgaggagaa
    25741 cacgtatgga ggtgggcaag ttggggaagc cgtattcgct gctgaatctg gcatatgtgg
    25801 gagtataaga cgcgcagcgt cgcatcaggc atttttttca ccaacgcaaa aaggccatcc
    25861 gtcaggatgg cctttcgctt
//
LOCUS       DAZGBC010000065        25586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGBC010000065 DAZGBC010000000
VERSION     DAZGBC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.065"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            251..1345
                     /gene="gcvT"
                     /locus_tag="ACHK1J_002543"
     CDS             251..1345
                     /gene="gcvT"
                     /locus_tag="ACHK1J_002543"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO7911793.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVEKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1369..1758
                     /gene="gcvH"
                     /locus_tag="ACHK1J_002544"
     CDS             1369..1758
                     /gene="gcvH"
                     /locus_tag="ACHK1J_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO7911794.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDVLSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1876..4749
                     /gene="gcvP"
                     /locus_tag="ACHK1J_002545"
     CDS             1876..4749
                     /gene="gcvP"
                     /locus_tag="ACHK1J_002545"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO7911795.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAGWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4915..6348)
                     /gene="bglA"
                     /locus_tag="ACHK1J_002546"
     CDS             complement(4915..6348)
                     /gene="bglA"
                     /locus_tag="ACHK1J_002546"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO7911796.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDILREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6393..6704
                     /gene="yqfB"
                     /locus_tag="ACHK1J_002547"
     CDS             6393..6704
                     /gene="yqfB"
                     /locus_tag="ACHK1J_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO7911797.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6868..7527
                     /gene="yqfA"
                     /locus_tag="ACHK1J_002548"
     CDS             6868..7527
                     /gene="yqfA"
                     /locus_tag="ACHK1J_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO7911798.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7604..8584)
                     /gene="ygfZ"
                     /locus_tag="ACHK1J_002549"
     CDS             complement(7604..8584)
                     /gene="ygfZ"
                     /locus_tag="ACHK1J_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO7911799.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            8827..9093
                     /gene="sdhE"
                     /locus_tag="ACHK1J_002550"
     CDS             8827..9093
                     /gene="sdhE"
                     /locus_tag="ACHK1J_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO7911800.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9074..9481
                     /gene="ygfX"
                     /locus_tag="ACHK1J_002551"
     CDS             9074..9481
                     /gene="ygfX"
                     /locus_tag="ACHK1J_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO7911801.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9521..10042)
                     /gene="fldB"
                     /locus_tag="ACHK1J_002552"
     CDS             complement(9521..10042)
                     /gene="fldB"
                     /locus_tag="ACHK1J_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO7911802.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10154..11050
                     /gene="xerD"
                     /locus_tag="ACHK1J_002553"
     CDS             10154..11050
                     /gene="xerD"
                     /locus_tag="ACHK1J_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO7911803.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11075..11785
                     /gene="dsbC"
                     /locus_tag="ACHK1J_002554"
     CDS             11075..11785
                     /gene="dsbC"
                     /locus_tag="ACHK1J_002554"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO7911804.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11791..13524
                     /gene="recJ"
                     /locus_tag="ACHK1J_002555"
     CDS             11791..13524
                     /gene="recJ"
                     /locus_tag="ACHK1J_002555"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO7911805.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13615..14713
                     /gene="prfB"
                     /locus_tag="ACHK1J_002556"
     CDS             join(13615..13689,13691..14713)
                     /gene="prfB"
                     /locus_tag="ACHK1J_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO7911806.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14723..16240
                     /gene="lysS"
                     /locus_tag="ACHK1J_002557"
     CDS             14723..16240
                     /gene="lysS"
                     /locus_tag="ACHK1J_002557"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7911807.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFGGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16283..16831)
                     /gene="idi"
                     /locus_tag="ACHK1J_002558"
     CDS             complement(16283..16831)
                     /gene="idi"
                     /locus_tag="ACHK1J_002558"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO7911808.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNPVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            16886..16957
                     /locus_tag="ACHK1J_002559"
     CDS             16886..16957
                     /locus_tag="ACHK1J_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911809.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16954..17079)
                     /gene="yqfG"
                     /locus_tag="ACHK1J_002560"
     CDS             complement(16954..17079)
                     /gene="yqfG"
                     /locus_tag="ACHK1J_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO7911810.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17081..18529)
                     /gene="uacT"
                     /locus_tag="ACHK1J_002561"
     CDS             complement(17081..18529)
                     /gene="uacT"
                     /locus_tag="ACHK1J_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO7911811.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18965..20884
                     /gene="ygfT"
                     /locus_tag="ACHK1J_002562"
     CDS             18965..20884
                     /gene="ygfT"
                     /locus_tag="ACHK1J_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO7911812.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHI
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGCDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20884..21372
                     /gene="ygfS"
                     /locus_tag="ACHK1J_002563"
     CDS             20884..21372
                     /gene="ygfS"
                     /locus_tag="ACHK1J_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7911813.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLLGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     LL"
     gene            complement(21408..22775)
                     /gene="ghxQ"
                     /locus_tag="ACHK1J_002564"
                     /gene_synonym="ygfQ"
     CDS             complement(21408..22775)
                     /gene="ghxQ"
                     /locus_tag="ACHK1J_002564"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO7911814.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22811..24127)
                     /gene="guaD"
                     /locus_tag="ACHK1J_002565"
     CDS             complement(22811..24127)
                     /gene="guaD"
                     /locus_tag="ACHK1J_002565"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO7911815.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24145..25545)
                     /gene="xanQ"
                     /locus_tag="ACHK1J_002566"
     CDS             complement(24145..25545)
                     /gene="xanQ"
                     /locus_tag="ACHK1J_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO7911816.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
BASE COUNT         6388 a         6435 c         6752 g         6011 t
ORIGIN      
        1 tcatcacatc atctttgtat agaaactggt gtgttttttg gttttttatt ctgtcgcgat
       61 ttttgcattt tttaaccata agctaatgtg atgatcaatt ttaccttatg gttaacagtc
      121 tgtttcggtg gtaagttcag gcaaaagaga acgattgcgt tggggaccgg gagtggctcc
      181 gatgctgggt tttgtggtga taatttcacc atgaaaaagt tgtcagcccc gcttattcaa
      241 tgaggacaag atggcacaac agactccttt gtacgaacaa cacacgcttt gcggcgctcg
      301 catggtggat ttccacggct ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca
      361 tcatgcggta cgtaccgatg ccggaatgtt tgatgtgtcg catatgacca tcgtcgatct
      421 ccgcggcagc cgcacccggg agtttctgcg ttatctgctg gcgaacgatg tggagaagct
      481 caccaaaagc ggcaaagccc tttactcggg gatgttgaat gcctctggcg gtgtgataga
      541 tgatctcatc gtctactact ttactgaaga tttcttccgc ctcgttgtta actccgccac
      601 ccgcgaaaaa gacctctcct ggattaccca acacgctgaa cctttcggca tcgaaattac
      661 cgttcgcgat gacctttcca tgattgccgt gcaagggccg aatgcgcagg caaaagctgc
      721 cacactgttt aatgacgccc agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt
      781 gcaggcgggc gatctgttta ttgccaccac tggttatacc ggtgaagcgg gctacgaaat
      841 tgcgctgccc aatgaaaaag cggcggattt ctggcgcgcg ctggtggaag cgggcgttaa
      901 gccatgcggc ctgggcgcgc gtgacacgct gcgtctggaa gcgggtatga atctttatag
      961 tcaggagatg gacgaaacca tctctccttt agccgccaat atgggctgga ctatcgcctg
     1021 ggaaccggca gatcgtgact tcatcggtcg tgaagccctg gaagtgcagc gtgagcatgg
     1081 tacagaaaaa ctggttggtc tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc
     1141 ggtacgtttt accgatgcgc agggcaacca gcatgaaggc attatcacca gcgggacttt
     1201 ctccccgacg ctgggttaca gcattgcgct ggcgcgcgtg ccggaaggta ttggtgaaac
     1261 agcgattgtg caaattcgca accgtgaaat gccggttaaa gtgacgaaac ctgtttttgt
     1321 gcgtaacggc aaagccgtcg cgtgatttac ttttttggag attgattgat gagcaacgta
     1381 ccagcagaac tgaaatacag caaagaacac gaatggctgc gtaaagaagc cgacggcact
     1441 tacaccgttg gcatcaccga acacgctcag gagctgttag gcgatatggt gtttgttgac
     1501 ctgccggaag tgggcgcgac ggttagcgcg ggcgatgact gcgcggttgc cgaatcggta
     1561 aaagcggcgt cagacattta tgcaccagta agcggtgaaa tcgtggcggt aaacgacgta
     1621 ctgagcgatt ccccggaact ggtgaacagc gaaccgtatg caggcggctg gattttcaaa
     1681 atcaaagcca gcgatgaaag cgaactggaa tcactgctgg atgcgaccgc atacgaagca
     1741 ttgttagaag acgagtaacg gctttattcc tcttctgcgg gagaggatca gggtgaggaa
     1801 atttacgcct caccctcact ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt
     1861 caggaaccat cgctcatgac acagacgtta agccagcttg aaaatagcgg cgcttttatt
     1921 gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa tgctgaatgc cgttggcgca
     1981 caatcgttaa acgcgctgac cggccagatt gtaccgaaag atattcagct tgcgaccccg
     2041 ccgcaggttg gcgcaccggc gaccgaatac gctgcactgg cagaactcaa ggctattgcc
     2101 agtcgcaata aacgcttcac gtcttacatc ggcatgggtt acaccgccgt gcagttaccg
     2161 ccggttatcc tgcgtaacat gctggaaaat ccgggctggt ataccgcgta tactccgtat
     2221 caacctgaag tctcgcaggg ccgccttgag gcactgctca atttccagca ggtaacgctg
     2281 gatttaaccg ggctggatat ggcctctgct tctcttcttg acgaagccac cgctgccgcc
     2341 gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa atgccaaccg cttctttgtt
     2401 gcttctgacg ttcatccgca aacgctggat gtggtacgta ctcgtgctga aacctttggt
     2461 tttgaagtga ttgtcgatga tgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg
     2521 ctgttacagc aggtcggtac tactggtgaa attcacgact acactgcgct tatcagcgaa
     2581 ctgaaatcac gcaaaattgt ggtcagcgtc gccgccgata ttatggcgct ggtgctgtta
     2641 actgcgccgg gtaaacaggg cgcagatatt gtctttggtt cggcgcaacg cttcggcgtg
     2701 ccgatgggct acggtggccc acacgcggca ttctttgcgg cgaaagatga atacaaacgc
     2761 tcaatgccag gccgtattat cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc
     2821 atggcgatgc agactcgcga gcaacatatt cgtcgtgaga aagcgaactc caacatttgt
     2881 acttcccagg tgctgctggc aaacatcgcc agcctgtatg ccgtttatca cggcccgatt
     2941 ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg atatcctggc ggcaggcctg
     3001 caacaaaaag gtctgaagct gcgccatgcg cactatttcg acactctgtg tgtggaagtg
     3061 gccgacaagg cgggcgtact ggcgcgtgcc gaagcggctg aaatcaacct gcgtagcgat
     3121 atcctgaacg cggttgggat cacccttgat gaaaccacca cgcgcgaaaa cgtgatgcag
     3181 cttttcagcg tactactggg ggataatcac gggctggaca tcgacacgct ggacaaagac
     3241 gtggctcacg acagccgctc tattcaacct gcgatgctgc gtgatgacga aatcctcacc
     3301 catccggtgt ttaatcgcta ccacagcgaa accgaaatga tgcgctatat gcactcgctg
     3361 gagcgtaaag atctggcgct gaatcaggcg atgatcccgc tgggttcctg caccatgaaa
     3421 ctgaacgccg ccgccgagat gatccccatc acctggccgg aatttgccga actgcaccct
     3481 ttctgcccgc cggaacaggc cgaaggttat cagcagatga tcgcgcagct ggctgactgg
     3541 ctggtgaaac tgaccggtta cgacgccgtc tgtatgcagc cgaactctgg cgcacagggc
     3601 gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa gccgcaacga agggcatcgc
     3661 gatatctgcc tgatcccggc ttctgcgcac ggcactaacc ctgcttctgc acatatggca
     3721 ggaatgcagg tggtggttgt ggcatgtgat aaaaacggca acatcgatct ggctgatctg
     3781 cgcgcgaaag cggaacaggc gggcgataac ctctcctgta tcatggtgac ctatccttcc
     3841 acccacggtg tgtatgaaga aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc
     3901 ggtcaggttt accttgatgg cgcgaacatg aacgcccagg ttggcatcac ctcgccaggc
     3961 ttcattggcg cggacgtttc gcacctcaac ctgcataaaa ctttctgcat tccgcacggc
     4021 ggtggtggtc cgggtatggg accgatcggt gtgaaagcgc atctggctcc gtttgtaccg
     4081 ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc agggcgcggt ttctgcggca
     4141 ccgttcggta gcgcctctat cctgccaatc agctggatgt acatccgcat gatgggcgca
     4201 gaagggctga aaaaagcaag tcaggtggca atcctcaacg ccaactatat tgccagccgc
     4261 ctgcaggatg ccttcccggt gctgtatacc ggtcgtgacg gtcgcgtggc gcacgaatgt
     4321 attctcgata ttcgtccgct gaaagaagaa accggcatca gcgagctgga tattgccaag
     4381 cgcctgatcg actacggttt ccacgcgccg accatgtcgt tcccggtggc gggtacgctg
     4441 atggttgaac caactgaatc tgaaagcaaa gtggaactgg atcgctttat cgatgcgatg
     4501 ctggctatcc gcgcagaaat tgaccaggtg aaagccggtg tctggccgct ggaagataac
     4561 ccgttggtga acgcgccgca cattcagagc gaactggtcg ccgggtgggc gcatccgtac
     4621 agccgtgaag ttgcggtatt cccggctggc gtggcagaca aatactggcc gacggtgaaa
     4681 cgtctggatg atgtttacgg cgaccgtaac ctgttctgtt cctgcgtacc gattagcgaa
     4741 taccagtaat tcactgattc gactctcttc taaaggcgct tcggcgcctt tttagcttgt
     4801 taataaagtg cgctttgtgt gtttcggctg aggcgtaata taaaatcttg cgagctcaat
     4861 atattaaaag agtgccaggc ctgatgagcg tatcgcatca ggcacatcgg ctaattacag
     4921 attctcgccg ttgctggcaa tcacctcttt gtaccagtta aagctcttct tacgtgaacg
     4981 cgacatatcg ccagtaccgt cgtcatgttt attcacatag ataaagccgt agcgtttgct
     5041 gtactgcccg gtggtgaacg acacgcagtc gatgcagccc cacggtgtgt agcccatcag
     5101 atccacgcca tcgtaagtca ccgctttttt catctcttcg atatgggcgc gcaggtagtc
     5161 aatgcggtag tcatcgttga tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc
     5221 gttttcgaca ataaacagcg gcctctgata acgctcatac agttcgcaaa gggcatagcg
     5281 cagacctact ggatcaatct gccagcccca gtcagacgct ttaacatacg ggttcggtac
     5341 gctgccttcg aaaccagaga tcgcatcgcc ggtgccgcct tcggccttca ctgcgttggt
     5401 catgtaatag ctgaaaccaa gataatcgca ggtgccttcg cgcagtatat cgagatcgcc
     5461 atcttccatt ttgatgttaa atccgcgacg ctcccactcg ttcaacacat aggacgggta
     5521 atagccgcgt agctgcacat cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa
     5581 catcacatcg tccgggttac aggagtaagg atagagcggc accatcgcca gcatacagcc
     5641 gactttcatc tccgggttaa tgcgacgcgc agctttcacc gccagggcgc tggcgacaaa
     5701 ctggtgatgc agcacctgat acatcgtctc ttccgggttc tcatgctcgg tataaaccac
     5761 gccggagcag cagtaaccga acagcggtgc acgccagtta cgctggttgt taatttcgtt
     5821 gaaggtcatc cagtatttga ctttatgctt atagcgttca aataccactt ccgcgaaacg
     5881 gacaaagaaa tcaaccactt tacggttggt ccagctaccg tattgctgca ccagatgcag
     5941 cggcatttca aagtgggaga gggtgatcac tggttcgatg ttgtatttca gcaattcatc
     6001 gaacagagag tcgtaaaact tcagcccttc ttcgtttggc tgagcttcat cgccttttgg
     6061 aaaaatgcgc gtccaggcga tggatgtacg aaaacatttg aagcccattt cggcaaatag
     6121 cttgatgtct tccttgtagt gaccataaaa atcaacggct tcatggtttg gatagtattt
     6181 tcctggcaag acttctttgg tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac
     6241 gtcacaaatg ctcggccctt tgccgccttt gttccagccg ccttcgacct gatgagcggc
     6301 aactgcgccg ccccataaga aatctttcgg taaggtgagt tttttcacta tcataggctc
     6361 cagatgagtc aattcttaca ggaaatagta ccatgcagcc aaacgacatc actttttttc
     6421 aacgtttcca ggatgacatt ctggctgggc gtaaaaccat caccatccgc gacgagtctg
     6481 aatcgcactt caaaacgggt gatgtgcttc gtgtcgggcg ttttgaagat gacggttatt
     6541 tttgcacgat tgaagtcacc gcaacctcaa ccgtaacgct ggatacgctg acggaaaaac
     6601 atgcagagca ggaaaatatg accctgactg aactgaaaaa ggtcattgcc gacatctatc
     6661 ccggtcagac acagttttat gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg
     6721 ttagttgaaa tttcgcattt aatttcatga tttttaggag attagtctca aatcagaatt
     6781 tattttagct aacaggtgtt cactggaact attctcagtt acgctggaga ggtatacatg
     6841 tgcgtgtacg tttctccgga gtaagttatg gttcagaagc ccctcattaa gcagggatat
     6901 tcgctggcag aggaaattgc caacagcgtc agtcacggca ttgggttggt gtttggtatc
     6961 gttgggctgg tgttgctact ggttcaggcg gtggatctca atgccagcgc cacagcgata
     7021 accagctaca gcctctatgg cggcagtatg atcctgctgt tcctcgcttc gacgctctat
     7081 cacgccattc ctcatcaacg ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt
     7141 tacctgttga ttgccggaac ctacacgccg tttttgctgg tggggctgga ttctccgtta
     7201 gcgcgcgggt tgatgattgt tatctggagc ctggcattgc tggggattct gttcaaactg
     7261 accatcgcgc accgattcaa aattttatct ctggtgactt atctggcgat gggctggctg
     7321 tcgctggtgg taatttatga aatggcagtt aagctcgcgg cgggcagcgt taccttactg
     7381 gcggtaggcg gcgtggttta ttcgctcggg gtgattttct acgtctgcaa acgcattcca
     7441 tacaaccatg ccatctggca cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg
     7501 atctatttgt atattgggca ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc
     7561 cagcgtcgca tcaggcgata aatgcctgat gcgacacaga gatttactct tcgagcgaat
     7621 acggcagcgg ctcgatatgc aatgtattcg catcgtcacg tacgcggaag atgctgtccg
     7681 gttccatatc gttattcatc actacctgta ccacgacctg accatcttcc agttttaccg
     7741 cagccagcac tgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac
     7801 cagcttccgg cagtcggctg gcactacctg tcagcaacca gagcgcacgt ttattggcac
     7861 cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga
     7921 taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttcgcggcat
     7981 caatcaccgg gaaacccgct tcaatgttta atgccagcca ctgttggcta ttgttcagtt
     8041 ccgcttcacc gcgcagttta tcggtcagca tattggcagt agcttcatcg gttacgatca
     8101 ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct ttaactacct
     8161 gtttttctct cgaaggtagt tcactaaaga gattggccag cgcggcgcgc gcctgaaaac
     8221 cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta gagaacaccg
     8281 catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccacgcaa
     8341 agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg gcgtcgcaat
     8401 gggcggcgag caggtgctga ttttctgtca tctggctgac atctgctgtc acctggccct
     8461 gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca
     8521 tcagcgtcag cggtaaacgg gcagaagccg taggctgacg tggaggaaaa ggtgtaaaag
     8581 ccataataga gtcctgagtt gcttaacggc gtattaataa ctaatggtaa aagagctatg
     8641 aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac ttccaaaaaa
     8701 atgaagccct tgaattgttg tgaattaatt tcgcgatcgc gattgtgttt actgatattc
     8761 ccgtaaaaga agtcgaaaaa cacgttacaa tagcggggta ctggattttc gtgagacagg
     8821 aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg tggtatgcgc
     8881 gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt aagcgatgac
     8941 gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cagacctgtt taactggctg
     9001 atgaatcacg gtaaaccggc cgatgcagaa ctggaaatga tggtccgact catccagaca
     9061 cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg
     9121 gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccgct
     9181 cagttacacc ccgttatgga tggtgttact ttcgctggtg gtgtttgatt gcgttcgcag
     9241 ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg
     9301 ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat
     9361 gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat
     9421 ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagata
     9481 aagatgacgg caaagcatat gcgctaagcc gtcagtgaca tcaggcgtaa tgctctgcca
     9541 tttcgttgag gatttgttcg caccagctct gaatacgctc gtcactaagg tcatactggt
     9601 tagtttcgtc cagcgccagc cccacgaaca gttgcccatc agcaatcacc ggtttcgggc
     9661 tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga
     9721 gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc
     9781 caagcccata cagcgcaaca attttacctt cgaggttcag gtcgtcgagc tgatcccaga
     9841 cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca
     9901 catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt
     9961 ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac
    10021 cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgcggct
    10081 agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc
    10141 ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg atgctctgtg
    10201 gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat
    10261 ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca
    10321 ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct
    10381 gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc
    10441 cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc
    10501 gcaggttgaa cgtttattac aggcaccatt aattgatcag ccactggagc tacgcgataa
    10561 agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcggactgac
    10621 aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga
    10681 gcgcctggta ccgttaggtg aagaggcggt ttactggctg gaaacctatc tggaacatgg
    10741 gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca
    10801 gcagatgacg cgacagacct tctggcaccg tattaaacat tatgccgtgc tggcgggtat
    10861 cgacagcgaa aagctgtcac cgcatgtgtt gcgccacgct tttgccactc atttattaaa
    10921 tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc tttccaccac
    10981 gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc
    11041 gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac
    11101 tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc aacaaacgtt
    11161 agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg gcatgaagac
    11221 agttctgact aacagcggtg tgttgtacat caccgatgat ggtaaacata tcattcaggg
    11281 gccaatgtat gacgttagcg gcacggctcc ggtcaatgtc accaataaga tgctgttaaa
    11341 gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg aaaaacacgt
    11401 catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc
    11461 agactacaac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga
    11521 tagcgatgct gagaaagaaa tgaaagctat ctggtgtgcg aaagacaaaa acaaagcgtt
    11581 tgatgatgtg atggcaggta aaagcgtcgc accagccagt tgcgacgtgg atattgccga
    11641 ccattacgca cttggcgtcc agcttggcgt tagtggtact ccggcagttg tgctgagcaa
    11701 tggcacactt gttccgggtt accagccgcc gaaagacatg aaagaattcc tcgacgaaca
    11761 ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt
    11821 gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg ccgtttatac
    11881 gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg tatgctgccc
    11941 tggcagcaac tgagcggcgt cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa
    12001 ggaacgcgga ttattgtggt cggtgatttc gacgctgacg gtgccaccag cacggctcta
    12061 agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt accaaaccgt
    12121 ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg
    12181 cagttaattg tcacggtgga taacggtatt tcctcccatg cgggggttga gcacgctcgc
    12241 tcgttgggca tcccggttat tgttaccgat caccatttgc caggcgacac attacccgca
    12301 gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca
    12361 ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgta cctttttgcg cgatcagggc
    12421 tggtttgatg agcgtaacat cgcaattcct aacctggcag aactgctgga tctggtcgcg
    12481 ctggggacag tagcggacgt cgtgccgcta gacgctaata atcgcattct gacctggcag
    12541 gggatgagtc gcatccgtgc cggaaagtgc cgtccgggga ttaaagcgct gctggaagtg
    12601 gcaaaccgtg atgcacaaaa actcgccgcc agcgatttag gttttgcgct ggggccacgt
    12661 ctcaatgctg ccgggcgact ggacgatatg tccgtcggtg tggcgctgtt gttgtgcgac
    12721 aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa
    12781 gagatcgaac aaggaatgca aattgaagcc ctgaccctgt gcgagaaact ggagcgcagc
    12841 cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca gggcgttgtc
    12901 ggtattctgg cttcgcgcat caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca
    12961 gcaggtgacg gtacgctgaa aggttccggt cgctccattc aggggctaca tatgcgtgat
    13021 gcgctggagc gattagacac actctacccc ggtatgatgc tcaagtttgg cggtcatgcg
    13081 atggcggcgg gtttgtcgct ggaagaggat aaattcgaac tctttcaaca acggtttggc
    13141 gaactggtta ctgagtggct ggacccttcg ctattgcaag gcgaagtggt gtcagacggt
    13201 ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg
    13261 tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct gcaacagcgg
    13321 ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg
    13381 gatggtattg cttttaatgt cgataccgcc ctctggccgg ataacggcgt gcgtgaagtg
    13441 caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct gcaaattatc
    13501 atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg tggattgggt
    13561 acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca gaccatgttt
    13621 gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga cgttcttagg
    13681 gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga
    13741 acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc
    13801 cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag atgtttctgg
    13861 tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag ccgttgctga
    13921 actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt tctctggcga
    13981 atatgacagc gccgactgct acctcgatat tcaggcgggg tctggcggta cagaagcaca
    14041 ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa
    14101 aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg tgactatcaa
    14161 aatctccggc gattacgctt acggctggct gcgtacagaa accggcgttc accgcctggt
    14221 gcgtaaaagc ccgtttgact ccggcggtcg tcgccatacg tcgttcagct ccgcgtttgt
    14281 ttacccggaa gtcgatgacg atattgatat cgaaatcaac ccggcggatc tgcgcattga
    14341 cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg
    14401 tattactcac atcccaaccg ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa
    14461 gaacaaagac caggccatga agcagatgaa agcgaagctt tatgaactgg agatgcagaa
    14521 gaaaaatgcc gagaaacagg cgatggaaga taacaaatcc gatatcggct ggggcagcca
    14581 gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgcactg gggtagaaac
    14641 ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag caagtttgaa
    14701 agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg acgcggtagt
    14761 cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc gcgagcaggg
    14821 gattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc acgcagaatt
    14881 cggcggtaaa gagaacgaag aactggaagc gctgaatatc gaagtcgccg ttgctggccg
    14941 catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg
    15001 ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca acgagcagtt
    15061 caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac
    15121 cggcgaactg tctatccact gtaccgagct gcgtctgctg accaaagcac tgcgtccgct
    15181 gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga
    15241 tctcatctct aacgatgaat cccgtaacac ctttaaagtg cgctcgcaga tcctctctgg
    15301 tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga tgatgcaggt
    15361 gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc tggatctcga
    15421 catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg gtggcttcga
    15481 gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atctccgtac gtcataaccc
    15541 agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc tgatcgagct
    15601 gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg aagtgaccta
    15661 cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat
    15721 caagaaatat cgcccggaaa ccgacatggc ggatctggac aacttcgact ctgcgaaagc
    15781 gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc gtatcgttac
    15841 cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca ttactgaata
    15901 tccggcagaa gtttctccgt tggcgcgtcg taacgacgtt aacccggaaa tcacagaccg
    15961 ctttgagttc ttcattggtg gtcgtgaaat cggtaacggc tttagcgaac tgaatgacgc
    16021 ggaagatcag gcgcagcgct tcctggatca ggttgccgcg aaagatgcag gtgacgacga
    16081 agcgatgttc tacgatgaag attacgtcac cgcactggaa catggcttac cgccgacagc
    16141 aggtctgggg attggtatcg accgtatggt aatgctgttc accaacagcc ataccatccg
    16201 cgacgttatt ctgttcccgg cgatgcgtcc ggtgaaataa gcattacgtt atgctcacaa
    16261 ccccggcaaa tgtcggggtt ttttatttaa gctgggtaaa tgcagataat cgtattctgg
    16321 cttcgcgatt tgtcgcctgc atcaccatcc acggactgaa cgcccacggc gtggcatcaa
    16381 taccgcgtaa tacatcagct aaatcacacc attgataatc catcacttca tcatcgttga
    16441 tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt tccacaatgc
    16501 cattcggatc ggtggcgcga tagcgaaagt caggatagat agattcagga ggcgtaattt
    16561 ccacgccaag ctcataacgg caacggcgga tcaccgcgtc ttcgttgctt tctcccagtt
    16621 gtgggtgccc acaaaccggg ttagtccaca cgccaggcca tgcttttttg ctcagtgcgc
    16681 ggcgggtaac taataattgt cctttggcat taaacagcca actggagaac gcgagatgta
    16741 agcgggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga actccctgtg
    16801 cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc tgatcactca
    16861 tcattataga taattgaaga gtgaaatgat taaccaagtg agcgtttatc gacaaccgcc
    16921 cgttttgagc ggatgtcgac aggtaaaaac catttaaaaa agtagcatca tatatttgaa
    16981 gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga gagtaaaaag
    17041 caacagcaga ctgaatatag cgcgcattaa aaaattcata ttattctcca tgctcatttt
    17101 tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttttaaa tctttatcag
    17161 agacggattc cttcaccagg tcagcatgat gctggtagcc attaaagaag acattcagca
    17221 caacggcgct aagggttgcg agcataatgc cgctatgcag cagcggttgc agtacggccg
    17281 gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg agactgatcg
    17341 ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga attcgaatcc
    17401 ctgtcgccag taccatgccg aacatcacca gcccagcgcc gcccagcaca aattgcggaa
    17461 tggaggctac cagcaccgcc atttttggca ccatgccaaa caggattaat ataattcccg
    17521 aagaaataca cacccagcgg ctatgaacgc gcgtcacgct aaccaggcca acgttttgag
    17581 aaaatgacgt atgggggaag ctgttaaacg tgccgcctat cattgtccct acgccatcga
    17641 cacgcagccc gcgaataata tcgtgtgaag agagtttacg accaactatt tcacccagtg
    17701 ccaggaacat ccccatcgac tcgataaaca cgatgattaa cacggcagtc atggtcagaa
    17761 tggaaacggg atcaaaaatc ggcataccaa acgacatcgg cgtaacaatc gcaaaccatg
    17821 aagcatcatg tagcccggat aaattgactt cattcatcat ccacgaaagt aaaaagccaa
    17881 atacaatccc cagtaatacg gcgacgttgg acataaatcc tttcgcatag cgagtaatga
    17941 gcaagataaa aattaagacg gcaaaggaga tacctaaata aacgggatta ccatattgcg
    18001 gattcccttt acctccggcg gcccagtcaa tacccacctg aatgatgcta agcccgatag
    18061 aagtaataac cacaccggta accagtggcg gaaataaagg catcaagcga ccgataagtg
    18121 gcgctaataa tgtggtgata aaacccgcgg cgatagtggc accaaatatc cccagcaggc
    18181 cgatatccgg gttcatccca atggctatca ttggtgttac agcagcaaag gtcaccgaca
    18241 taatcaccgg caggcggatc cccataaagc ggccgatacc gatacattgc aataatgtga
    18301 cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc aatagcatct tttgagaggc
    18361 ccagtcggtc accaatcata agaggaacag cgactgcacc tgcgtacatg acaaggacgt
    18421 gttgcagacc gagtatgatc agctttcctg gtgataatat gcggtcaacc tcatcagttg
    18481 ggcggtcttg cccagaagat gagggaagtt gggaatctat ggcgctcatc agtgtttctc
    18541 ctttcgcccg ccagagtaaa aagcaaccgt ttacgttaat gaaactgtct aaacacatgc
    18601 tcgtggagtg ccagtttttg cgaggaaggg gagttagaga caggaattgc ggaggcaatc
    18661 acataatcgt gcttaaacca cgaaaatgag aagagaacct gacaatggaa ttatctgtaa
    18721 ttttctcata ttgatatttc attaatcatt ctggcatgtc gcactctcgc atttaatcgt
    18781 ttttatctgg atagcgctct tttgatcggc aatgttgaaa taatgaaagc ggcatagatt
    18841 gaggggcaat aatttgaact cagtcgcaaa attataatta aaacggtcat gctacctaat
    18901 atatgcccgt ttattgcatc gcgaaatttg agaagacata actcattaaa tgaaagggat
    18961 gcaaatgaat aagtttatcg ctgctgaagc tgcggaatgt ataggctgtc atgcttgtga
    19021 aattgcctgt gcggtggcac acaatcaaga aaactggccg ctgagtcaca gtgactttcg
    19081 accacgtatc cacattgtag ggaaaggcca ggctgcgaat ccggtggcct gccatcactg
    19141 caacaatgcc ccttgcgtta cggcttgtcc ggttaatgct ctgactttcc agtccgatag
    19201 cgtacaactg gacgagcaaa aatgtattgg ttgtaaaaga tgcgcaatcg cttgcccctt
    19261 tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc gacctttgta accagcgcag
    19321 ttccggcacg caagcctgta ttgaagtttg cccaacacag gcgttacgac tgatggacga
    19381 taaagggtta cagcagataa aggtggcccg ccagcgcaaa acggcagcag gaaaagcgtc
    19441 atcagacgct cagccatctc gcagtgcagc gttgctcccc gttaactcgc gtaaaggcgc
    19501 agataaaatt tcagcaagtg aacggaaaaa ccattttggc gaaatctatt gcgggctgga
    19561 tccacaacaa gcgacttatg agagtgaccg ctgtgtttat tgtgccgaaa aagctaactg
    19621 caactggcat tgcccgctgc ataacgctat tccggattac atccgtctgg tacaggaggg
    19681 aaagattatt gaagcggcag aactttgcca ccagaccagt tccttaccag aaatctgcgg
    19741 cagggtatgt ccacaagacc gtctttgtga aggcgcatgt actttgaaag atcactctgg
    19801 cgcagtctct atcggtaatc tggaacgcta catcaccgat accgcgctgg cgatgggctg
    19861 gcgtcccgat gtcagcaaag ttgttccccg tatcgagaaa gtggcggtga ttggcgctgg
    19921 gcctgcaggg ttagggtgtg ctgatattct ggcgcgcgca ggagttcagg tcgatgtctt
    19981 tgatcgccat ccagaaattg gcggtatgct gacttttggc attcctcctt tcaaactcga
    20041 taaaacggta ttaagccagc ggcgagagat attcaccgca atgggaatcg actttcatct
    20101 taactgtgaa attggctgcg atattacctt tagcgattta acttctgaat atgatgcagt
    20161 tttcatcggc gtggggactt acgggatgat gcgagctgat ctgccgcatg aagatgcgcc
    20221 cggtgtcatt caggctctac cgttcttgac tgcccatacc cgccagctca tggggttgcc
    20281 ggagtctgaa gagtatccgc tgacggacgt ggaaggtaag cgagtcgtgg tattgggcgg
    20341 tggcgatacg acaatggatt gtttgcggac ttccatccgc ctcaatgccg ccagcgtgac
    20401 ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg cgcaaagagg tggtcaatgc
    20461 gcgcgaggaa ggtgtcgagt ttcaattcaa tgttcagccg caatatatcg cttgtgacga
    20521 agatgggcgt ttaactgcgg tgggcctgat tcgtaccgcc atgggtgagc cggggccgga
    20581 tggtcgccgt cgtcctcgtc cggttgcggg ttcagagttt gaattgcccg ccgatgttct
    20641 cattatggcc tttggtttcc aggcgcatgc catgccgtgg ttgcagggca gcggaattaa
    20701 actcgataaa tggggcctga ttcaaaccgg cgacgttggg tatttaccta cccagacgca
    20761 tctgaaaaaa gtctttgctg gtggggatgc agttcatggc gcggatctgg ttgtcactgc
    20821 catggccgca ggaaggcagg cggcgcgcga tatgttaact ctgtttgata cgaaggcatc
    20881 gtgatgaaat cgttaattat cgttaatccg gctgactgta ttggctgccg tacttgtgaa
    20941 gtggcctgtg tggtcgctca cccttcagaa caggagttga atgccgatat ctttttaccc
    21001 cggctgaagg tacagcgact ggactgcatc agtgcgccgg tgatgtgcca ccaatgtgaa
    21061 aacgcccctt gtgttggcgc ttgccccgtg ggggcgctga cgatgggcga gcaggtggtg
    21121 cagaccaatt ctgcccgttg tattggctgt cagagttgcg tcagtgcgtg cccgtttgga
    21181 atgatcacca ttcagtcgtt gctgggggat acccggcaac aaatcgtgaa atgcgatctc
    21241 tgtgaacagc gggaagaggg gcctgcctgc gttgaatctt gcccaacaca ggcgctgcag
    21301 ttgcttactg aaagagaact caggcgagtc cgccagcagc gtattgttgc cagcggtgag
    21361 aatcttctct gaaagaaatc tccgcgctga aaggcgcgga gaatctatta gattgcccaa
    21421 ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca ccgtaccaat attcagcttt
    21481 tgccattcgc gtgcaaagac gcggcctacg accagtgtca caaagcccag cataataccg
    21541 gtaacgatat tacaagtcag aacgatgaac acggcacaca ccaggccagc catagcgtca
    21601 ataaaatcat tgaaatccag cttcgagacg ttacttaaca tcagcaaacc tacgtacatc
    21661 agagcgggtg cagtggcgta accagggatc aaaaatgaca gcggtgataa gaacagaatc
    21721 agcaggaata acgcccccac tacggttgcg gttaaccctg ttttaccccc ggcggcggtt
    21781 cctgccgctg attcgatata aaccgctgcg ggcgctgcgc ccaccaggcc ggagaatatt
    21841 gaacttacag agtcactggt cagggctttg ccgccgttga tgatctggtt gtctttatcc
    21901 aacaaattcg cctgaccggc gacggcacgg atggtgccag tagcgtcgaa cactgcggtc
    21961 atcaccaatg ccagtacact cggaagtaca gttggctgga gtgcgcccat aatatcgagg
    22021 ctgaaaatca gagacttacc atcttcgcca gtcaggcttg gcatcgccac cagaccgtgg
    22081 tatttcaccg ctgggtcaaa gattaagccg atgatcgaaa ttgcaataat caccaacaag
    22141 atcccgccgg gtacgcgaca cttctccaga ccgaagatga ccgccagccc cagcaagctc
    22201 atcatcaccg ggaaggaggt aaacgcaccg agcgccactg gcaagccttc aatcgggttt
    22261 ttgataacca tacccacacc gttagcagca atcagcagca gaaacagccc gataccgata
    22321 cctgtaccgt gagcgatacc catcggcaaa ttacgtaaga tccaggtacg cacaccggtt
    22381 acggaaatgg cggtgaagat gacgcccatc agaaataccg cgcccagtgc gacaggaacg
    22441 ctaatttgtt gcccgagtac cagactgaat gcggtaaacg ccgtcaagga aatcgcgcaa
    22501 ccaatcgcca ttggcaaatt agcccataac cccatcagca acgagccgaa gcccgcgacc
    22561 agacaggtgg caacaaacac agctgcggga ggaaaacctg ctttgcccag cattcccgga
    22621 acgacgataa cggaataaac catggccaga aaggtcgtta agccagccag tacttcctga
    22681 cgaacagtac tgccacgagc ggtaatttta aaataattat ccagcgcgcc ctgtggcttt
    22741 ggtgcgtccg gtgtttgtag gatgtctcca gacataatga tgtcctcacg aggatgttaa
    22801 agttttatta ttagttgcgt tcgtacacca gacgaccatc aacgtaggtg cggtagatcg
    22861 aacggtcatc gcccaacgtc atcatcacga acaatttgtc gactaaagaa acagagttgt
    22921 catagcgcag ctgctgtagc ggagtggcgg tgggttccat caccacgaaa tcagcctctt
    22981 tgccaggtaa aaagttgcca atcaaatcgt caaggcccag agatttcgct ccgccgagcg
    23041 tggccaggta aaacgcttca tatgccgaga ggcgatagcc ttgtaattgc aacactttgt
    23101 aggcttcgtt cagcgtttgc agcatgttga aagtggttcc ggcaccgata tccgttccca
    23161 tgcccacttt aactttcttc tgccatgctt ttttcaagtt gaataacccg ctgcccaggt
    23221 aaaggttgga ggtcggacag aaagcaatgc tggatttggt ttcgctgaga cgatcccact
    23281 ctttttcttc gagatggacg cagtgagcaa agacacagtt tttaccggtc aggccgtact
    23341 gatggtaaac atccagataa ccatcatggt caggataaag cgatttcacc caggcaattt
    23401 catctttgtt ttcacagaga tgggtatgta cccacgtatc cggatattcc tctttcaggc
    23461 gttgcgccat cgccatctgt tcaggagatg aggtaggggc gaatcgtggc gtaatcgcat
    23521 acaacagacg accatttttg tgccagcgtt cgatcagttc tttgctttgg tgatagctgc
    23581 tttcggcagt gtcgagcaga taatccggtg cgttacggtc catcatcacc ttaccggcaa
    23641 tcatacgcat attgatatga ctggcggctt caaacagcgc atcaacggat tgcggatgaa
    23701 cagtgccaaa caccagcgcc gtggtggttc cgttacgtaa aagctgcttg atgaagaacg
    23761 ccgacatttc gcgggcgtac tctaaatcct cataacgacg ttcagtaggg aaggtgtgtt
    23821 tattcaacca ctccagcaat tgctcaccat aggcccccac catttcactt tgcggataat
    23881 ggatatgtgt atcgacaaag cccggtacta tcagtttgcc gcgatagtcg cgcacgcgaa
    23941 tggtgtcagg aatttgatgc tttccgtttt cccattcgcc aaaccattcc acttttccct
    24001 gtttaatgag taataaacca tcctcaataa accgcagcgc agaggcaatc tcttccgggt
    24061 tatcgaccgt acgggtgaca tcaataaaac tgcctcgtac cgcttttaac gtgtgttctc
    24121 ctgacatcat tgactccttt actgttaatc catctcttcc gctgaggtaa taccaggcag
    24181 aacgttttcc tgtcggtagc caccagggag gataatattt aataaaatcg cagttaaccc
    24241 gccagcacaa atagggtttt caactaatac ataaatagag gctggcaata ttttaaaaat
    24301 ttcgggatcg taggagacgc caagccctaa acctaaagaa gtggcgacaa taagtgtttc
    24361 acgccgcttt aatccgttgg tgatgatgat gcgaatccca gcgatggcaa tcatggaaaa
    24421 catcaacgtc attgcgcctc ccagaactgc cgagggaatg gtcgtgaaga agccgccaat
    24481 catcggaaat aagccgagga taaccagcat taccgcgatg gttcgcccga cataacgtga
    24541 agcgacgcca gtcatctgaa taaccccatt attttgcgca aacgtggtta atggtaatga
    24601 accaacagcg gaggcgataa cagaaaccag accatctgcc agcacgccgc ctttcagtcg
    24661 ggactgatac tcttccccct gaatggggcg gcgggaaacc attgccgtgg cggtgatatc
    24721 gccgactgct tccagcacgc taagcagata aatcgtgcca accaccagga actgatggaa
    24781 gctaaaacta aagccgtatt tgaacggatg cgggatggtg attaacggca aattgcgcat
    24841 actgctgaaa tccaccatgc ccaggcataa cgatgcgata tagccgacac atagcccaat
    24901 ggcgatccct cccatgcgta gcaacggact gcgacagcag ttaaagccga tcaccacaat
    24961 taaaaccaat aaaccaacgc cgagatgttc gtaattgccg aacgtaccgc tgcttttggc
    25021 tgcaaatcct ccaccaaaat cgataatgcc gactttaatc aggcttaagc cgatcatcag
    25081 taccacaata ccgctgacgg taggcgtaat aacccgacgt aaatagggca agataaatga
    25141 agatccgaca accagaaatg cgccaacgaa ggagacgccg agaagcgacg acatgattaa
    25201 ctcttcgtga aaaccgtcgc ttttcatgct gctgcccagc gcaatcatga ccgtaacaaa
    25261 tgaaaaattg actgactgaa ttgagagtag gccagaaccg acgatgccgt agcggtttac
    25321 ttgtaaccag gtaccaatac cagaggcgat catcgccata gaaacaagat aggcggttgt
    25381 ttcagcggaa agctgtaagg ccgcacccac gattaacgcg ggggttacca tcggaacgaa
    25441 aattgccaac agatgggtaa tggcaccaac gagagcctga tgaaagggag ggcgatcctc
    25501 cagttcaaat ataaggtcag aacctgcatg gtttatatca gacatccctt cactcctttt
    25561 aaagtaaatt tgatcatctg aattag
//
LOCUS       DAZGBC010000066        25197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGBC010000066 DAZGBC010000000
VERSION     DAZGBC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.066"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(197..280)
                     /gene="yohP"
                     /locus_tag="ACHK1J_002567"
     CDS             complement(197..280)
                     /gene="yohP"
                     /locus_tag="ACHK1J_002567"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO7911817.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            504..1940
                     /gene="mdtQ"
                     /locus_tag="ACHK1J_002568"
     CDS             504..1940
                     /gene="mdtQ"
                     /locus_tag="ACHK1J_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO7911818.1"
                     /translation="MNRDSFYPAIACFPLLLMLTGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSELFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1993..2754
                     /gene="yohF"
                     /locus_tag="ACHK1J_002569"
     CDS             1993..2754
                     /gene="yohF"
                     /locus_tag="ACHK1J_002569"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7911819.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2884..3462)
                     /gene="yohD"
                     /locus_tag="ACHK1J_002570"
     CDS             complement(2884..3462)
                     /gene="yohD"
                     /locus_tag="ACHK1J_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7911820.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKIIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3632..4219
                     /gene="yohC"
                     /locus_tag="ACHK1J_002571"
     CDS             3632..4219
                     /gene="yohC"
                     /locus_tag="ACHK1J_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO7911821.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4393..5325
                     /gene="pbpG"
                     /locus_tag="ACHK1J_002572"
     CDS             4393..5325
                     /gene="pbpG"
                     /locus_tag="ACHK1J_002572"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO7911822.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5364..7079)
                     /gene="dld"
                     /locus_tag="ACHK1J_002573"
     CDS             complement(5364..7079)
                     /gene="dld"
                     /locus_tag="ACHK1J_002573"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7911823.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            7275..9572
                     /gene="bglX"
                     /locus_tag="ACHK1J_002574"
     CDS             7275..9572
                     /gene="bglX"
                     /locus_tag="ACHK1J_002574"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO7911824.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSSQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9824..10741
                     /gene="osmF"
                     /locus_tag="ACHK1J_002575"
     CDS             9824..10741
                     /gene="osmF"
                     /locus_tag="ACHK1J_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO7911825.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLGRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10748..11905
                     /gene="yehY"
                     /locus_tag="ACHK1J_002576"
     CDS             10748..11905
                     /gene="yehY"
                     /locus_tag="ACHK1J_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO7911826.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKFGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11898..12824
                     /gene="yehX"
                     /locus_tag="ACHK1J_002577"
     CDS             11898..12824
                     /gene="yehX"
                     /locus_tag="ACHK1J_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO7911827.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVVDYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            12829..13560
                     /gene="yehW"
                     /locus_tag="ACHK1J_002578"
     CDS             12829..13560
                     /gene="yehW"
                     /locus_tag="ACHK1J_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO7911828.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13541..13648)
                     /gene="yohO"
                     /locus_tag="ACHK1J_002579"
     CDS             complement(13541..13648)
                     /gene="yohO"
                     /locus_tag="ACHK1J_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO7911829.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13708..14439)
                     /gene="mlrA"
                     /locus_tag="ACHK1J_002580"
     CDS             complement(13708..14439)
                     /gene="mlrA"
                     /locus_tag="ACHK1J_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO7911830.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGYDIFPLGI"
     gene            14661..16346
                     /gene="btsS"
                     /locus_tag="ACHK1J_002581"
     CDS             14661..16346
                     /gene="btsS"
                     /locus_tag="ACHK1J_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO7911831.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16343..17062
                     /gene="btsR"
                     /locus_tag="ACHK1J_002582"
                     /gene_synonym="yehT"
     CDS             16343..17062
                     /gene="btsR"
                     /locus_tag="ACHK1J_002582"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO7911832.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            17109..17579
                     /gene="yehS"
                     /locus_tag="ACHK1J_002583"
     CDS             17109..17579
                     /gene="yehS"
                     /locus_tag="ACHK1J_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO7911833.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17620..18081)
                     /gene="yehR"
                     /locus_tag="ACHK1J_002584"
     CDS             complement(17620..18081)
                     /gene="yehR"
                     /locus_tag="ACHK1J_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO7911834.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(18206..20209)
                     /gene="yehQ"
                     /locus_tag="ACHK1J_002585"
     CDS             complement(18206..20209)
                     /gene="yehQ"
                     /locus_tag="ACHK1J_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO7911835.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMAQRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSAGVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHMLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(20206..21342)
                     /gene="yehP"
                     /locus_tag="ACHK1J_002586"
     CDS             complement(20206..21342)
                     /gene="yehP"
                     /locus_tag="ACHK1J_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7911836.1"
                     /translation="MSELNDLLTNRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARNFDFKSTLRANLQHWHPQHSKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVNLTADVADPVELLMKVQ
                     LGGGTSIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21335..23614)
                     /gene="yehM"
                     /locus_tag="ACHK1J_002587"
     CDS             complement(21335..23614)
                     /gene="yehM"
                     /locus_tag="ACHK1J_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO7911837.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFLGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQSWCWQWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESDGKPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHVPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLN
                     DDDFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(23625..24713)
                     /gene="yehL"
                     /locus_tag="ACHK1J_002588"
     CDS             complement(23625..24713)
                     /gene="yehL"
                     /locus_tag="ACHK1J_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO7911838.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDAAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5903 a         6451 c         6841 g         6002 t
ORIGIN      
        1 atccccttcg gctaccgggt cgggcgcgag gaaacgtcct tgtaagacgc gccctcagcc
       61 cacatccatg tgggctgccc cggccttcgg ggaacgcttc agcgattttg acgccaccaa
      121 caccggagct gtttgcaaat caagtacaca aaatatctag ttcctgacct gacaacttat
      181 atgtcatatc tgatttttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa
      241 tcaggaaaat aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga
      301 gtgctgaata agtataggaa aaaacgaagc cctttgccgt tatccataaa cgattaagat
      361 aaatcggaag atatcattca cttagtgtat atttttctgg atatgtcgtt ttattgctac
      421 tgatgtattg ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc
      481 ttttcttacc tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt
      541 ttccgctgtt actgatgctg accgggtgtg cgcctatgca tgaaacccgc caggcgttaa
      601 gccagcaaac gcccgctgca caagttgata ccgcattacc cacggcgctg aaaaatggtt
      661 ggccagacag ccaatggtgg ctggagtatc acgataatca actcacttca ttaattaaca
      721 atgcactaca gaacgcaccg gatatgcagg tggctgaaca gcgcattcag ctagctgaag
      781 cccaggcgaa ggcaattgcc actcaggatg gtccgcagat cgacttttct gcggatatgg
      841 agcggcaaaa aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg
      901 caggtacgac cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc
      961 tcgatatctg gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac
     1021 gggcggcgga acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact
     1081 gggagtggca aacccaggcg gcgttaaaca cggtcttgca gcaaatcgaa aaagagcaga
     1141 acaccattat cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgaag
     1201 gtgtggaaac cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga
     1261 aaatgaaaat tattgaggca cggttaagcg cacttacaaa tcaccagaca aagtcattga
     1321 agcttaaacc ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact
     1381 ccttactggc ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgctaa
     1441 gcaccattga tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc
     1501 aacaggatgc gttgcactta agcgagctgt tccgtcattc cgcgcagcaa atgggcgtta
     1561 cggcaggcct gacgctaccc attttcgata gtggtcgtct taacgccaat ctcgatatcg
     1621 caaaagccga aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcggtga
     1681 atgacgtggc gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg
     1741 cgcaaattga gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg
     1801 gcatcattgc tggttcccgc gtcagcgaag ccagaatccc cgcgctgcgt gagcgggcca
     1861 atggcctgtt attgcaaggg cagtggctgg atgcctccat tcaactcact ggtgcgttgg
     1921 gcgggggtta caaacgctga tgatatgtga cttctatact taacgtttat tcagcgttaa
     1981 gtggagaact cgatggcaca ggtagcgatt attaccgcct ccgattcggg gatcggcaaa
     2041 gagtgcgcgt tattactggc gcagcaaggg tttgatattg gcattacctg gcactcagat
     2101 gaagaagggg caaaagatac cgcgcgtgag gtagttagcc acggcgtacg tgcggagatt
     2161 gtgcagctgg atctcggtaa actgccagaa ggtgcgcagg cgttggagaa actcattcaa
     2221 cggctggggc gtattgatgt gctggtgaat aatgcgggcg caatgaccaa agcgccgttt
     2281 cttgatatgg ctttcgatga gtggcgcaag atttttaccg ttgatgtcga tggtgcattt
     2341 ttatgctcac aaattgcggc ccgccagatg gtgaaacaag ggcagggcgg tcgcatcatc
     2401 aacattacgt cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct
     2461 aaacatgcgc tcggtgggtt aaccaaagca atggcgctgg agctggtcag acataagatt
     2521 ttggtgaacg cagtcgcgcc tggggcgatc gccacgccaa tgaatggcat ggatgacagc
     2581 gacgtgaagc ccgacgcgga gccttcgatt cctctgcggc gttttggcgc aacgcatgag
     2641 attgccagcc tggtagcgtg gctgtgctca gagggcgcaa attacaccac cgggcagtcg
     2701 ttgatagtgg atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt
     2761 gaggtgcgtg gttatttcaa tgggtaaaat tttggtaggc atgataagac gcacaagcgt
     2821 cgcatcaggc atggtgcccc ggatgtcgga tgcggcgcga acgccttatc cagggcaatg
     2881 gttttacgcc tgattatccg gctttttctt cccgcgccgt ttcagccacc agcgcacgcc
     2941 taccaccaga accacgacca gaatcaacca gacccagtgc ttcaaatgct gatcgagatt
     3001 gtgcaaccac ggcgcaatca cctgaccacc agcgtaaccg atagtggtaa aaatcaacgc
     3061 ccaggcaaat gcgccgagaa tattcagcgg cagaaagatt ttcggcggca gctggctggc
     3121 accaatcagc gtcgggccaa tcacccgaaa gccatacata aagcgcgtac caatgacaaa
     3181 cagatacgga tggcgttgga taattttctg cgcccgctca attttatcct gatgtttcga
     3241 gaaacggcgt aacagcttgc cgccaaaccg ccgcccgcac agataaagca cctggtcgcc
     3301 aatcatgccg ccaagcgcca cagaaagtac caccagcggg aactttaata gcccctgatg
     3361 cgccgcaacg cctcccagca aagtcacggt ttcaccttcc gccaggctac cgatcaccag
     3421 cgcggcataa ccatattgtg agatgagtgt attgagatcc ataggccagt ctgtctccct
     3481 ttggttgcct gtaagcatac accctggaaa tcattcacgc tgaaaaagga cgtacatcag
     3541 acggtgcaaa ttacatgagc gaacaaaaaa taatcacaag ctgacttata cttgaactgt
     3601 caggttcatg aactcacaac aagggggcgg tatgagccat gtctggggac tgttttccca
     3661 tcccgatcgt gaaatgcagg tgattaatcg cgaaaacgaa acgatttctc accattacac
     3721 ccaccacgtt ttgctgatgg cggcgattcc ggtgatttgc gccttcattg gcactacaca
     3781 gattggctgg aattttggcg atggcactat cctgaagtta tcctggttta ccggactggc
     3841 gctggctgtc ttgttttacg gcgttatgct ggctggggtt gcggtgatgg ggcgggtcat
     3901 ctggtggatg gcgcgcaatt acccgcagcg tccgtcactt gcgcactgca tggtcttcgc
     3961 gggctacgtt gcaactccgc tgtttttaag cggtctggtg gcgctttatc cgctggtctg
     4021 gttgtgtgcg ctggtcggca cggtggcact gttttacacc ggttacctgc tttaccttgg
     4081 tattccgagc ttcctgaata tcaacaaaga ggaaggcctt agcttctcca gttcgacgct
     4141 ggcgattggc gtgctggtac tggaagtgct gctcgccctt accgttatac tatggggcta
     4201 cggttatcgt ttgttctaga aagttctttg acgttgcatt gctggcgtaa atgtcagcaa
     4261 tgcagcattt cctcacgatt ctcctttgac gatcgcactt tttgctcgtt atgatggcgt
     4321 cgctagcctc agtaaatcct tactccggcg gtgcgcaacc cgtgcgcgtg aaccactatc
     4381 tgaatgctca tcatgccgaa atttcgagtt tctttattta gcctggccct gatgctggct
     4441 gtgcctttgg caccgcaggc cgttgcgaaa acggcagccg ctaccaccgc ttcacaaccg
     4501 gaaattgcct ccggtagcgc gatgattgtt gatctgaata ccaacaaagt gatctattcg
     4561 aaccacccgg atctggtgcg tccgattgcg tctatcagca aattaatgac cgcgatggtt
     4621 gtgctggatg cgcgactgcc gctggatgaa aaactaaaag tggatatcag ccagacgccg
     4681 gagatgaaag gggtctattc gcgcgtacga ctgaacagcg aaatcagccg taaagatatg
     4741 ctgttgctgg cgttgatgtc ttcagaaaac cgcgcggcgg caagccttgc gcaccattat
     4801 cccggtggtt acaaagcctt tattaaggcg atgaatgcga aagcgaaatc gctcggaatg
     4861 aacaacacgc gctttgttga accgaccgga ctatcggtgc ataacgtttc aaccgcccgt
     4921 gacttaacca aactgctcat tgccagcaaa caatatccgt tgatcgggca gttaagtacc
     4981 acccgggaag atatggcaac cttctctaac ccgacgtata cgctgccgtt ccgcaatact
     5041 aatcatctgg tgtatcgcga taactggaat atccagctaa ccaaaaccgg ctttaccaat
     5101 gcggcgggcc attgtctggt gatgcgtacg gttatcaata ataaaccggt ggcgctggta
     5161 gtgatggatg cgtttggcaa atatacccat tttgccgatg ccagccgcct gcgtacctgg
     5221 attgaaaccg gtaaagtgat gcctgttccg gcagcagcgt tgagctataa aaaacaaaaa
     5281 gccgcccaaa tggcggcggc ggggcagacg gcacagaacg attaagtgaa ttagggatgg
     5341 cgatactctg ccatccgtca tttttactcc acttcctgcc agtttttccg cttactggtt
     5401 ttaccgatcc ccggattcat actgttggtc ggatcgttct cgcgataaaa cttctgcaac
     5461 gtctccggtg ctttatacaa atgaccgacg ttatgttcgg cagggtattg cgcgccgcgc
     5521 tgctgtagca gctccagcat ctgttctttc aacgcatgca catccacgcc ttttttaacg
     5581 atgtaatcct gatggaagac atagcacata aaatggccgt aatagagctt gtgcaccagc
     5641 tggctgtcga tctccggcgg taaatgctca taccactcgg tgtcgttacg ccgcagagcg
     5701 atatccagcg ccagaatgtc ttcgacttca tcggaatgca ccgcctgata acgaattgct
     5761 gcgcccgcag cggcgaaacg gtgtaaaaac gctttgctgc cttcctccgg cgtacagaca
     5821 aagaaatcgc cctcggcctg tttgaaataa tccaccagcc acgatttggc ttcaccaacg
     5881 ccatcgcccg ccatttttaa cagcagatga tgctcgtatt tatcgcgcca gtttttcatg
     5941 cgcggcggta aatggctggg gaacaggtga ccgaattttt gcatcgcacg gtcagtaaaa
     6001 tgcggacgga agaatttcac tttctccagc atcgcatcgg tgcgtccctt gagattaaag
     6061 aagaacggca tcttgtcggt gcctaactta tcaatcatca ggaaggtgtc tttgccgtat
     6121 ttttccgcaa tatcgtagat atcccggtgc atatattccc cggcaaccgg cagattttcg
     6181 aagttagcca gaatatgacg gcggatttcg gtcagcactt ccggctggtt ggtgccgata
     6241 taaaacacct gctgattttt ttccgcttcg aaggtatcaa gacgtactgc aaagacggcc
     6301 agcttcccgg cgcaaccaga agattcaaat aaccgatccg gatcggcgtt ataacgtgcg
     6361 ggcgtgtcgg cttcaatatc acgaacgcgg tggacatagt cataatcgtg ggcgtgacga
     6421 ccatcgtgac gcacatcgtc atctttgatg cgatcgtcat ccagcttgct aaggatctgc
     6481 tccggcgttt cgcccagatc aatccccaga tggttcacca gcgtcagttt gccatcttca
     6541 tttatacgcg cgaataacga catttcggta tacgccgggc cacgctgcac cagcgaacca
     6601 ccagagttat tacagatacc gccgatgacc gatgcgccta tacacgatga tccaatcacc
     6661 gagtgcggtt cgcgtcccag cggtttgagg gctttttcca gcgaatagag cgtggtgccc
     6721 ggataggcca gcacctgttc gcccttgccg agaacgtgca gcttgtcgag acgcagggtg
     6781 ctgatgatca cgatatcgcg atcataatcg ttaccgtttg gcgtcgatcc ttcggtcagg
     6841 cctgtattgg cagcctgcat cagaataatt ttgtcggcgg tgacgcaggc tttcagcacc
     6901 cgccacaatt ccagtagtga gccagggaaa acgacagcca gcgcgtcgcc ctgaccagaa
     6961 cggaagccct tgcgatagcg ggccgttttt gcgggatcgg tgagcaggtg tgaatgaccg
     7021 accagacggg caagttcatt caaaaaggct ttattatcag ttgttgtcat ggaagacatt
     7081 ttccactcct tgtggtggcg aaaaaatatc acaaactaga atagcgcctt gtatgacaat
     7141 tcagcgagtg aatattttga aaattcagac aataaactga gcattctttt tctctctcca
     7201 tcatgcttat ggcaaactac ggtttttgca ccattttcgc tgccacgtcg ggcaacaaag
     7261 gaagaaaaat ccatatgaaa tggctatgtt cagtaggaat cgcggtgagt ctggccctgc
     7321 agccagcact ggcggatgat ttattcggta accatccatt aacgcctgaa gcgcgggatg
     7381 cgttcgtcac cgaactgctt aagaaaatga cagttgatga gaaaattggt cagctgcgct
     7441 taatcagcgt cggcccggat aacccgaaag aggcgatccg cgagatgatc aaagacggtc
     7501 aggttggagc gattttcaac accgtaaccc gtcaggatat ccgcgccatg caggatcagg
     7561 tgatggaatt aagccgcctg aaaattcctc ttttctttgc ttacgacgtg ctgcacggtc
     7621 agcgcacggt gttcccgatt agcctcggtc tggcctcgtc ttttaacctc gatgcagtaa
     7681 aaacggtcgg acgtgtctct gcttatgaag cggcagatga tggcctgaat atgacctggg
     7741 caccgatggt cgatgtctcg cgcgatccgc gctggggacg tgcttccgaa ggttttggcg
     7801 aagatacgta tctcacttca ataatgggta aaactatggt ggaagcgatg cagggtaaaa
     7861 gcccggcaga tcgctactcg gtgatgacca gcgtcaaaca ctttgccgct tacggcgcgg
     7921 tagaaggcgg taaagaatac aacaccgtcg atatgagttc gcagcgcctg tttaatgatt
     7981 atatgccgcc gtacaaagcg gggctggacg caggcagcgg tgcggtaatg gtggcgctga
     8041 actcactcaa cggcacgcca gccacctccg actcctggct gctgaaagat gttctgcgcg
     8101 atcagtgggg ttttaaaggc atcaccgttt ccgatcacgg tgcaatcaaa gaactgatta
     8161 aacatggcac ggcggcagac ccggaagatg cggtgcgcgt ggcgctgaaa tccggaatca
     8221 acatgagcat gagcgacgag tactactcga agtatctgcc tgggttgatc aaatccggca
     8281 aagtgacgat ggcagagctg gacgatgctg cccgccatgt actgaacgtt aaatatgata
     8341 tggggttgtt taacgaccca tacagccatc tcggtccgaa agagtctgac ccggtggata
     8401 ccaatgccga aagccgcctg caccgcaaag aagcgcgtga agtggcgcgc gaaagcctgg
     8461 tgttgctgaa aaaccgtctc gaaacgttac cgctgaaaaa atccgcaacc attgcggtgg
     8521 ttggcccgct ggcagacagc aagcgtgacg tgatgggcag ctggtccgca gccggtgttg
     8581 ccgatcaatc cgtgaccgtg ctgaccggga ttaaaaatgc cgtcggtgaa aacggtaaag
     8641 tgctgtatgc caaaggggcg aacgttacca gtgacaaagg cattatcgat ttcctgaatc
     8701 agtatgaaga agcggtcaaa gtcgatccgc gttcgccgca agagatgatt gatgaagcgg
     8761 tgcagacggc gaaacaatct gatgtggtgg tggctgtggt cggtgaagct caggggatgg
     8821 cgcacgaagc ttccagccgt accgatatta ctattccgca aagccaacgt gacttgattg
     8881 cggcgctgaa agccaccggt aaaccgctgg tgctggtgct gatgaacggg cgtccgctgg
     8941 cgctggtgaa agaagatcag caggctgatg cgattctgga aacctggttt gcggggactg
     9001 aaggcggtaa tgcaattgcc gatgtattgt ttggcgatta caacccgtcc ggcaagctgc
     9061 cgatgtcctt cccgcgttct gtcgggcaga tcccggtgta ctacagccat ctgaacaccg
     9121 gtcgtccgta taatgccgac aagccgaaca aatacacttc gcgttatttt gatgaagcta
     9181 acggggcgct ttatccgttc ggctatggtc tgagctatac cactttcacc gtctctgatg
     9241 tgaaactttc tgcgccgacc atgaagcgtg acggcaaagt gacggccagc gtgcaggtga
     9301 cgaacaccgg taaacgcgaa ggggccacgg tagtgcagat gtacttgcag gatgtgacgg
     9361 cttccatgag tcgtccggtg aaacagctga aaggctttga gaaaatcacc ctgaaaccgg
     9421 gcgaaactca gaccgttagc ttcccgattg atatcgaggc gctgaagttc tggaatcaac
     9481 agatgaaata tgacgccgag cctggcaagt tcaatgtctt tatcggcact gattccgcac
     9541 gcgttaagaa aggcgagttt gagttgctgt aatgctttag taagggcgcg acgtttgcgc
     9601 cctttgcctg atgttcatat cttaacttgc tgaattttcg ccctttgtag gccggataag
     9661 gcgctcgcgc cgcatccggc aggaaacgcc ggaccgaata tgcctcttaa gtatttatct
     9721 gattctgcaa ctgaatccgt ccgctcaggc taactctgtc attatcaact atgcttttct
     9781 cttaattcgc tgaaaacaac agtaaaagaa tgaggaaagc atcatgccac tctcaaaggt
     9841 ctgggcaggt tcactggtta tgttggcagc cgtgagcctg ccgctacagg cagcttctcc
     9901 cgttaaagtc ggttcaaaaa tcgataccga aggcgcgctg ctcggcaata tcattttgca
     9961 agtactcgaa agccacggcg taccgacggt gaataaagtg caactcggaa cgactcctgt
    10021 ggtgcgcggg gcgattactt ccggtgaact ggatatctat ccggaatata ccggcaatgg
    10081 cgctttcttc tttaaagatg aaaacgatgc ggcatggaaa aacgcgcagc aaggttacga
    10141 gaaagtcaaa aaactcgatg cagagcaaaa caagttaatc tggctgacgc ctgcaccggc
    10201 gaataacacc tggaccatcg ccgtgcgtca ggatgtggca gagaaaaaca aactcacttc
    10261 gcttgccgat ctcggtcgtt atctgaaaga gggcggcacc ttcaaactgg cagcctcggc
    10321 agagtttatc gaacgcgccg atgcgttacc cgcgtttgaa aaagcctacg actttaaact
    10381 cgatcaggat cagttactgt cactggctgg cggcgacacg gcggtaacga ttaaagccgc
    10441 tgcccaacaa acttctggcg ttaatgccgc aatggcttac ggcactgacg gtccggtcgc
    10501 ggcgctgggg ctgcaaacct taagcgatcc gcaaggcgtg caacctatct acgcgcctgc
    10561 accagtggtg cgtgaatcgg tgttgaagga gtatccgcaa atggcacagt ggctacagcc
    10621 agtcttcgcc agcctcgatg aaaaaacatt gcagcaactg aatgccagca ttgcagttga
    10681 aggactggat gccaaaaaag tggctgccga ctacctgaaa caaaaagggt ggacgaagta
    10741 atttcccgtg acttatctac gtatcaatcc tgttctggcg ctgctgctgt tgctgacggc
    10801 aatcgcagcg gcgctgccgt ttatcagtta cgcgcctaat cgtttagtct cgggtgaagg
    10861 gcgacatctc tggcagctgt ggccgcaaac gatttggatg ctggtgggcg ttggttgcgc
    10921 ctggctgaca gcctgtttta ttcccgctaa aaaaggcagc atttttgcac tcattctggc
    10981 gcaattcgtc ttcgtattgc tggtgtgggg agcaggaaag gcggcgaccc aactggcgca
    11041 aaatggcagt gcgctggcgc gtaccagcct cggcagtggt ttctggctgg ctgcggcgct
    11101 ggcattgctg gcctgtagcg atgccatccg ccgaatctcc acgcatccgc tgtggcgctg
    11161 gttgttgcat atgcagattg ccattattcc gctgtggttg ctgtactccg gcacgcttaa
    11221 cgatctctca ctaatgaaag aatacgccaa ccgtcaggat gtgtttgacg acgcgctggc
    11281 acaacatctg acgttgctgt ttggtgcggt gctgcctgcg ttagtgattg gtgtgccgtt
    11341 gggcatctgg tgctattttt ccactgcgcg gcagggggca attttttctc tgctcaatgt
    11401 cattcagacc gtgccttcgg tggcgctctt tggcctgctg attgcgccgc ttgccggact
    11461 ggttacggcc tttccgtggc tggggaagtt cggcatagca ggaaccggaa tgacacccgc
    11521 actgattgcg ctggtgctct atgccttgct gccgctggtg cgcggcgtgg tagtaggctt
    11581 gaaccagatc ccgcgcgatg tgctggagag cgccagagcg atggggatga gcggggcgca
    11641 gcgattcctg catgttcagt taccgctggc gttaccggta tttctgcgca gcctgcgggt
    11701 ggtgatggtg caaactgtag gcatggcggt gattgcggcg ttaatcggcg caggcggttt
    11761 tggtgcgctg gttttccagg ggctgctaag cagcgccatt gatttagtgt tgctgggggt
    11821 gatcccggta attgttctgg cggtgctgat cgatgcgctg ttcgatttac ttatcgcact
    11881 gttgaaggtg aaacgtaatg attgaattta gccatgtcag caaactgttc ggcgcacaaa
    11941 aagccgttaa cgatctcaat ctcaattttc aggaagggag tttttcggtg ctgattggca
    12001 catctggttc cggcaaatcc accaccctga aaatgattaa ccgcctggtg gaacatgaca
    12061 gcggagagat ccgctttgcc ggagaagaaa ttcgctcgct gccagtactg gagttgcgcc
    12121 gccgtatggg ctatgccatt caatctattg gcctgtttcc ccactggagc gtggcacaaa
    12181 acattgccac cgtgccgcaa ttacaaaaat ggtcgcgggc gaggattgac gatcgtatcg
    12241 acgaattaat ggcgctactg gggctggagt caaatttacg tgagcgttat ccacatcagc
    12301 tttccggtgg tcagcagcaa cgtgtgggag tggcgcgcgc actggctgcc gatccgcaag
    12361 tcttactgat ggatgaacct tttggcgcac tggacccggt aacgcgcggc gcgttgcaac
    12421 aagagatgac gcgcattcac cgtttgctgg ggcgcactat tgtgctggtc actcatgata
    12481 ttgatgaggc gctacggctg gcagaacatc tggtattgat ggatcacggt gaagtggtgc
    12541 agcaggggaa tccgctgacg atgctgactc gtccggcgaa tgattttgtc cgccagtttt
    12601 ttggacgtag tgaactgggt gtgcgcctgc tttcgttacg tagtgtggtg gattacgtgc
    12661 gtcgcgaaga acgagcagaa ggtgaggcac tggcagaaga gatgacgcta cgcgatgcgc
    12721 tctctctgtt tgtcgcgcgg ggatgcgagg tgctgccggt ggtgaacacg cagggccagc
    12781 cttgcggcac gctgcatttt caggatctgc tggtggaggc gtaagcgtat gaagatgttg
    12841 cgcgatccgc tgttctggct cattgctttg tttgtggcac tgattttctg gctgccttac
    12901 agccagccgc tgtttgctgc cttgttccca caactgccac gacccgttta tcagcaagaa
    12961 agttttgcag ctctggcact ggctcatttc tggctggtgg gaatttcgag tttgtttgcg
    13021 gtgatcattg gtactggtgc cggaattgct gtcactcgcc cgtggggcgc ggaatttcgc
    13081 ccactggtgg aaactattgc cgccgttgga cagacttttc cgcccgtcgc ggtgctggcg
    13141 atcgccgttc cggtgatcgg gtttggtctg caaccagcga ttatcgcctt gatcctttac
    13201 ggtgtgctgc ccgtcctgca ggcgacactt gccgggctgg gagcgattga tgccagcgtg
    13261 acagaagttg cgaaaggtat gggaatgagt cgtggtcagc gactgcgtaa ggtcgagcta
    13321 ccgctggcgg ctccggtgat tctggcgggc gtgcgaactt cggtgattat caacattggt
    13381 acggcgacga tagcctcaac ggtaggggcc agcacgctgg gtacgcccat catcatcggg
    13441 cttagcggat ttaataccgc gtatgtgatc cagggggcgt tactggtggc actggcggcg
    13501 atcatcgcag accgcctgtt tgaaaggctg gtgcaggcgc ttagccagca cgcaaaataa
    13561 aggtataacc tgcgagcatg acgccaccaa ttccgcctaa cgccataaac aggaacaggg
    13621 cgatgacccc aattttagct atgcgcataa tgcactcctt atgttaacga aaggattgta
    13681 cagtaaagcg catttgttaa cgaatcatta aatgccgagt gggaaaatat catagccttg
    13741 ttcttgccaa ctggtgagtt gctgctgttg ggcggaggtt cgattttcac cgcaccacac
    13801 cagcaatgta cggccttcga acagttcagg gcgtagttga ttgagtgagt gggcgaggac
    13861 atcaatgcgc cagccttgtt gactggcaat ccagccctcc agccacagac gggtggtatc
    13921 gtgaatattc cagccaacca ccagcgcatc tttaccctgt tttttacgtg ccgaagccag
    13981 acaaatggcg atgtagttga tcagtacgcc gtcgaggatc gccagcagcg cctggagagt
    14041 cggttgttgg cactgaagcc gtcggcgcag aggaataaac agatgtgtgg tgagcgtctg
    14101 ggcggggtaa tcctgaccgc gctctttgat ccacgttcgc aggctatgca gattgccgct
    14161 ttgcaggtag gtcagtaatg tttcttgctg atcgcgccag ccgttctgca catcaacatt
    14221 ttcattactg agcagcattt taactttgct gacctgcacg ccgttgtcga tccagcgttt
    14281 gatctcgcgg atccggtcaa tatcggcatc gttgaacagc cgatgaccgc cgtctgtccg
    14341 ttgcggtttc agcaatccgt aacgcctctg ccacgcgcgt aacgtgacag ggttaatatc
    14401 acaaagcaac gccacttcac caattgtgta aagcgccatc gtctcaccct tgctcgcgag
    14461 gtcccggttt aactttagac gcagttttgc gaaccaggta gttttgcccg ttttttgtgc
    14521 atttataggg tgattttatt tttgccaggc gattttgagt gatcgtactc acgaattctc
    14581 atttttctgc aagagttcaa agaaagttaa acgcaggcaa tgtatgttac gcgttttaaa
    14641 gggaagtgtg gtttgcgggt atgtacgatt ttaatctggt gttgctgctg cttcagcaga
    14701 tgtgcgtttt tttagtcatt gcgtggttaa tgagtaaaac gccattattc ataccgttaa
    14761 tgcaggtcac ggttcgtctg ccgcataaat ttctctgcta catcgtcttt tccatcttct
    14821 gcatcatggg cacctggttt gggttgcaca ttgacgattc tattgccaat acccgtgcga
    14881 taggcgcggt aatgggcggc ttactcggcg gtccggtcgt cggtgggctg gttggcctga
    14941 ccggcgggtt acatcgatat tcgatggggg gcatgaccgc gctaagttgc atgatctcga
    15001 ccatcgttga aggattgctc ggcggcctgg tacacagcat cctgatccgt cgcgggcgca
    15061 ctgataaagt ctttaacccc attaccgccg gtgccgtcac gttcgtcgct gaaatggtgc
    15121 aaatgttgat catcctggcg atcgcccgac cttatgaaga tgcggtgcgt ctggtgagta
    15181 atattgctgc gccaatgatg gtcaccaata ccgtcggcgc ggcgctgttt atgcgtatat
    15241 tgctcgataa acgcgcgatg tttgaaaaat acacttcagc tttttctgcc actgcgctga
    15301 aagtggcggc ctcgacggaa ggcattttgc gccaggggtt taacgaagtg aacagcatga
    15361 aagtggcaca ggtgctgtat caggagctgg atattggtgc agtcgcgatt accgatcgag
    15421 agaaattgct ggcctttacc ggaattggtg acgaccacca tttacccggc aaaccgattt
    15481 cttcgactta taccttaaaa gcgattgaaa ccggtgaagt ggtctacgct gatggcaacg
    15541 aagtacctta ccgttgctct ttgcatccgc aatgcaaact ggggtcgacg ctggtaattc
    15601 cgttacgtgg tgaaaatcag cgggtgatgg gcaccatcaa attgtatgaa gccaaaaacc
    15661 gtttattcag ttcaatcaac cgcacgttgg gtgaggggat tgcgcaacta ctttcggcgc
    15721 agatccttgc cgggcaatat gagcggcaaa aagcgatgct cacccagtca gagatcaaac
    15781 tgcttcacgc ccaggtgaac ccccattttt tgtttaatgc gcttaacacc attaaagcgg
    15841 tgatccgccg cgacagcgaa caggccagcc agctggtgca gtatctttcc acttttttcc
    15901 gcaaaaactt aaagcggcct tcggagtttg ttactctcgc cgacgaaatt gaacatgtga
    15961 atgcttatct gcaaattgaa aaggcgcgct tccagtcgcg gttgcaggtc aacattgcta
    16021 ttccgcaaga attatcccag cagcaattgc ccgcgtttac cctgcaaccg atagtggaaa
    16081 acgccattaa acatgggaca tcacaactgc tggatacagg gcgagtggca atcagcgccc
    16141 gacgtgaggg gcaacattta atgctggaga tcgaagacaa tgccggtttg tatcaaccgg
    16201 taaccaatgc cagtgggctg gggatgaatc tggtggataa gcgtttacgt gaacggtttg
    16261 gcgatgacta tgggataagc gtcgcctgtg agcctgatag ttacacccga ataacgttac
    16321 gactaccatg gagggacgag gcatgattaa agtcttaatt gtcgatgatg aaccgttagc
    16381 acgggagaac ctgcgcgtat ttttgcagga gcagagcgat attgaaatcg ttggtgagtg
    16441 ttcaaacgcc gtggaaggga tcggcgcggt gcataaactg cgcccggatg tgctgtttct
    16501 cgatatccag atgccgcgca tcagtggtct ggaaatggtg gggatgcttg acccggaaca
    16561 tcgcccgtat attgtttttc tcaccgcgtt tgacgaatac gccattaaag cctttgaaga
    16621 acacgccttt gattatctgc tgaagccaat tgatgaagcg cgactggaga aaacgctggc
    16681 gcgtttgcgg caggaacgca gcaagcagga tgtttcgctg ttaccagaaa atcaacaggc
    16741 gctgaaattt atcccttgta ctgggcatag tcggatttat ttgctgcaaa tgaaagatgt
    16801 ggcatttgtc agcagtcgga tgagcggtgt ctacgttacc agccacgaag ggaaagaggg
    16861 ctttaccgaa ttgacattac gtaccctgga aagtcgtaca ccactactgc gctgccatcg
    16921 tcagtatctg gttaacctcg cgcatttaca ggagattcgt ctggaagata acggccaggc
    16981 cgagttgatt ttgcgtaatg gcttaaccgt gccggtcagc cgccgttatc tgaaaagctt
    17041 aaaagaggcg attggcctgt aaaagactgc taaaatggct ttttgcctca tcaacacctg
    17101 aaggcctcat gctaagtaac gatattctgc gcagcgtgcg ctacattttg aaagccaata
    17161 ataatgacct ggtgcgtata ctggcgctgg gtaatgtcga agccaccgcg gaacagatcg
    17221 ccgtctggct acgtaaagaa gacgaagagg gttttcagcg ttgtccggac attgttttgt
    17281 cgtcattcct caatggcctg atttatgaaa aacgcggcaa ggatgagtct gctccggcac
    17341 tggagccgga acgtcgcatt aataacaaca tcgtgctgaa aaaattacgc atcgcgtttt
    17401 cgctgaaaac cgatgacatt ctggcgatcc tcaccgaaca gcagttccgc gtttcgatgc
    17461 cggaaattac agcgatgatg cgcgcaccgg atcataaaaa cttccgcgaa tgcggcgatc
    17521 aatttttacg ttattttctg cgtggactgg cagcgcgcca gcatgtgaag aaaagctaag
    17581 acgggtatgg cggccatgcg aaacatggcc gccgacagat tatttcactt ctttaaaacc
    17641 agcggctttc atcaccagtt ccatttgcgc catagtgata ccttttttgg catcttcagc
    17701 agaaacgttg attcctgaaa taccctgcag ggctttaaaa tccacttttt ccatatcgat
    17761 agtcacgttt tcctgcgcgt aggtatcggt ataggttaat ttttcttcaa cacccgcgat
    17821 gtttttgtat ttggcgctta acggctcaag tgtcttggca gcatcttctt tggtggttgc
    17881 accaatggag gcaaattgaa ttttggtttc agaagattgc ttaagcacct tgtcaccttt
    17941 gtagacatag gtaatggcaa tttcagtgcc gttcagattg gcgctgaatt tcttcgattc
    18001 ttctttgtca ccgcagccag caagagagaa aaccagaaca gatgcaacaa caagggaaaa
    18061 cagcttattg aaagccttca tgtaaaactc cattttattt aatcaaggaa ctggtgactc
    18121 tcaccagggg ctatatagga tatgcctaat accgtggcgt gagcagtccg gaactggagt
    18181 agaactctta gtaaaaagca ctatttcatc cttgttgctg aagcatgggg aataattgtt
    18241 cgcacagcaa aacaccgtta ttcatagctt ctacccgtgc ctcgctttct gtattacgaa
    18301 attgtcccaa catatgtgcc agccgataaa aacccaccgc ggtgaggtca ttcgccagca
    18361 actctgcctg accaatagca ctctgttcct gatagcgcca gccgttatgg agcagttgaa
    18421 taagtaatgc ctggcagcgc atcagcaact gatgagcagt agacggcaca ggcagaacgc
    18481 cggcagaagg tagtgatgcc accacaggcg cagtttctgc gtccagcgcc caggcacgag
    18541 tctttgtcat cattacctgt ggttccagtg tcaattgccc atcaacaaaa ctgacaaagc
    18601 cagaaaccag acacacgggg tcgtctgttt gttgcaaaag cgccgccatg cgttcaacgg
    18661 cataaggcgc gctggctgag gctggcagtg ataacgtcag cagattatct tcaccttcgc
    18721 cgctgattac ctgcgcatcc agcgtctggc ggctgctatc ccaaccgagc gaaatacact
    18781 cagcgaccgg cagaataaat aagttatcga cctgattaag aggccgtatg caggaggggg
    18841 gacgctggcg taaatattcc cgcaaagcca caatgcccgg ctggcgtaac ggcgcgctca
    18901 acattcgcca ggcatcaggc gacagcggca caacgctgct taagcggttg cgggtagcta
    18961 acagcagctc gccatcggca ctgcgttttg ctgcttgtga aacaatttgc ccacccgcca
    19021 gtgcgccagc ctgaaaacta aacagccgac gcgtagctgc cggtgagttt tcctgttcac
    19081 ttcgcggcca actgcgcgaa aggtgcaaaa tactgccggt gtcgggatcg gtaaaccaga
    19141 tgcgtaaacc ataatgctca atatcctgcc agcaacgcat acctaaagac accagccgca
    19201 gatgatcaag ctttgcttct ccggcaatgc cagagccaac gaccgtgcgc cacggcacag
    19261 gaggaacttc accaacactg tcgcgctggg ccatctcttg tgcgcaattt aatcgactgt
    19321 ttaatgccgc aagctgacgt aagcattctc cggcatgata gtggctggcg cgggcgtgga
    19381 aggcatcaac gctggcgcgc agttgccgta gcgattcact cacccatcgc cagttgcagc
    19441 gttccgctgc ttgctgcgcg cggctgaaag cggcctcgta gtgaataagc ggctggctga
    19501 tgccgcccag ccataatgcc tggcttagtt gctgaacata ttgacgacac gttttgcctt
    19561 cttcgctggc aaacgggtcg tcagatgatg tgacgtgttc gctgcgcatc tgccagatta
    19621 aatgagtcaa ttccgcttgc tgcgcttttg cctggacaaa ggcctgtact gccagtacga
    19681 catgttcgca aagtgtgcct tcaatacaat cacaacgggc gaaacgaatg ctgctacggg
    19741 aataaaaacg cacatcgctc atcggtaagc gggcagaggg aatttcgccc ggcgtacaga
    19801 acaactcaat ggtgatgcct ttagcgacca gcgcctgtgc gcgtttgcgg gtggcatcgg
    19861 gaagggtagc cagttcttcc agccagattg ccggatccca ttcctcttct tttcccgtag
    19921 gctgggcggt agcacaaagt cgttgataac ttaacaccag catcacgcga tgacggcaca
    19981 tgccgctggc cccgcagctg cactgagcct ctttcagtgc ctgaccgttc gccagctggg
    20041 tacggacacc gtcactgaag gtggcgatta aagcgccgtt ctcatggctg atttccggga
    20101 cgttgccatt ttccagttcc ttaaggctgc gcttaacaaa accggcattg cttaacgccg
    20161 tcagtgcctg cggtgtcagt tctaataatt ccggacgtag tgaattcatg actgaagatt
    20221 ctccgcaagc catgatgcca gctcgcccgg cgtcatggcg gctatttgtg cgccgacatt
    20281 aaccagcgcc tgggccgtat cgcggtcata gcaaggcgtt gctgtgctat cgagcgctgc
    20341 cagtcccagc actttgatgc cgctctggac acactttttc acctgatgcg tcagcaatga
    20401 tgatgaaccc ccttcgtaaa aatcgctcac gaggataatg acgctttttg ctggttgttc
    20461 aataagttgc cgcccatact ccacggcact ggcgatactg gtcccgccgc ccaactgtac
    20521 tttcattaat aactctaccg gatcggcaac gtctgccgtg agattaacga cgcttgtgtc
    20581 aaacgccacc agatgggtac gaatgccggg taactgccac aaacaggccg ccatcaccgc
    20641 agagtggatc accgaatcga ccatcgatcc gctttgatca accagtaaga ccagttgcca
    20701 ttgttcactg tggcgcttaa tacggctgtt aaagcggggg gattcgatat acaacttgct
    20761 gtgttgtggg tgccagtgtt gcaggttggc gcgtagagta cttttgaaat caaagtttcg
    20821 cgccagtgga atagatgagc ggcgacggcg atcgcggaca ccagaaaaag cctgacgaac
    20881 ttcctttgcc agtcgagcca taatttcttc aacaacctgg cgcactatcc ggcgggcggc
    20941 agccagtact tcggggttca tcagatgttt ggtgtgcaaa acggcgcgta gcaggctttc
    21001 agaaggctgc atacgttcca gcacgtcgag attcgtcacc acatcttcaa tgccatagcg
    21061 cagtacggca tcgctttcca gccgctcaat cacctgttgc ggaaacagcg tgtgaatact
    21121 gttgatccac tcaggggtgg tgagatttga gccacctaat ccaccggagc gttcaccacg
    21181 ctgtagccgt tcaggatcgc gcccatacag ccactccagc gcgtggtcta tctgccgggc
    21241 gttgtcatcc agcccacaaa gcgtcgtttc tgccgcttcg ccaagaatta atcgccagcg
    21301 ttgtagctca cggttggtca gaagatcgtt cagttcagac atggaaaact ccccagtttt
    21361 gcagcgatgc cagtgcctgc tgttcgagtt gttgatgatg tgcaatggct tgtggtggac
    21421 aatgcaacgg catttgcagc gccgaaacgg gaagttgcgc cagttgataa tgctcaagca
    21481 cctgatgcgc cagcgtcccg cgttctcgtg gtggtagcca ggccatcgcc gcgcgtaaat
    21541 cgggcagggc gttaataaaa tcgtcgtcat tcagttgatt taaatgactg ctgaaaccgg
    21601 cgataaatgc cggctggcag gccagttgat gacgggcgag cgccagcaaa ccgtgcagcg
    21661 cctcgccgga ttgtgccggg gataactgcg ccagcattgt cagcgccgct tcggcactgg
    21721 cgttgggatg ttccagacgc atcagtgcgc caagagccgc gccgcgatcc agcgccggga
    21781 catgaattgc ctgactgcgg cgttccagta aggccaccgc cgcagaaaga gaaacgccgg
    21841 gtaaattaac gccgctatgt agatcgcgca ggatatggca aagcgcctgc cagctatgca
    21901 ggtgagcgtg aaactccttt tcatccggtt tgccgtcgga ttcacacagc cacagcgtgc
    21961 gatcgatagc cgcacataac gtcgtctgca atatctgcgc gccttgcata ccgctgattt
    22021 catctaaccg ccataaggca taaaggactt ccagcgcctg ccccatttcg gcaaattgat
    22081 tttcctgggc gattaataat gtgagttgct ccagtaattg ttgactgaag gacgccagac
    22141 ccgctaacgc cgcctggcta agacatgtgg tgatactgcc gattccaccc gcgtccagca
    22201 tatcggcttc taatttatgg cgtgcggctt cctggagtgt ggcaccaaag caggcggcct
    22261 caatcaatgc cgcatgctgg ctaagcgggc gggttaattt ccagcgttct tcaccgttgc
    22321 ctgcaagtgt cagtgtgctt ccctgctggc gtacaatccc tgggatctcc agtattgcca
    22381 gccgatgtaa cacctgactt tgcgctagtc cattggggtt aaagcgattt aaggtaagct
    22441 ccgccggaag agagattgcg gtacgttcca gttcgcaggt gacatctttt ggcagaggcg
    22501 gttgtggcgt agaaggggca agtttaccga atccgtcacc cgctaatgtg tctatcaacg
    22561 ttagcagaat cggatcggtg tcgggatgaa taactccgcg atagctccac ggcaacggcg
    22621 cgttcagggc ttctttaatc agcgagcctg ctatggcatc cagccagtca gtgcgtaacg
    22681 gtagtttatg tccgcgcaac tgtgccagcg ccatcgcatg cagatgagcg gcagccatat
    22741 ccgcggtcga agcaggcaag tggtgttggc gcaaacgggt aagaaccgtt tttagcagtt
    22801 gttctcccgc ctgctgtaag ccccactgcc agcaccagct ttgccagacc ggggcaggca
    22861 ttcctgaaag gtatcctgcc agcacatcaa ggcgcttttc actgtagggt gtgagataac
    22921 aacctgtaac ggcatctgcc agcgagggca attctggctt gttaatgtcc tgcgggcatt
    22981 cgcgccacat ttttgccagt gccggagcgt gccagccacc gcagacgact aacacgtcgc
    23041 cattattttg ctgcaccgcc catgcaatcc agcgagccat aaaggcttcg cgctgacgat
    23101 tgagtgcatc gccgaggaaa tcgccccgca gttgggcaaa atagtgcgcc agtgcagagg
    23161 gtaatgcagt ttgctggctt tcatcttcga acaagtgatc ccacagggta tcgctgttat
    23221 ccatgcgggt ggcacgcagc agcaaggtct ggctttcctc ttgcgtatca ggcgaatcgt
    23281 cctcttcttc actctgtgcc cagcaaggca aatcgatgaa gtaagtttgt gcctgaatac
    23341 gacgagcggc ttgtagcgcc tgccactccg gcgaaaattc agcaaatggc gtccaggcac
    23401 cacgcccggg ggctgcaccg tcctgatact ggcaataact gtaaatagct accggaagct
    23461 ggtgggctaa aaacagttcg tctacccggt cattaaaatc agccgggcct tcaatcaaca
    23521 cgtatcgtgg ccgctggctt tcgattaacg atttcaccag ccgggcgcag gccggactat
    23581 gatgccggat gccgacaatt aacggctcgc tcatgcatct ttcctcacgg caggcggtgg
    23641 cgggcttgat aataagcctg ccagtgagct tctttatgtg tggcgacacg ctgttcgaag
    23701 taacggcgca gacgagcgcg atcttcttcg ttatctttga cgatggttcc ggcaatgcag
    23761 tccaccagat cggctggctc acctgcgcga ttcgctaaaa accaggcgcg gacgcccacg
    23821 gcgtgggcca cgttcacggc ttcagcggtg gacataatcg ctgttagtgt atccattgag
    23881 gttttctttt cgccgttggc acgcagatcg cggaaggtgc ggaccagtaa ctccagtacc
    23941 gcgtcaggca ctttatgcgg aataccgcta tgggccagca aacgcgccga agcactggcg
    24001 acaagctcca gctcctgggc gaaatccata atcggaaaga cagtttcaaa atcgaagcga
    24061 cgctttagcg cagcactcat ttcattgacg ccgcgatcgc gggtattggc agtggcaata
    24121 atattaaagc cttctcgcgc atagagctga ctggcttcac cagtgagttc cggcaccgtc
    24181 atcaccctgt cagaaagcat acctaacaga cagtcttgca cttccagcgg tgtacgcgtt
    24241 atctcttcaa aacggacgat tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc
    24301 agcgcttccg ttgatggacc atgattgata agcagggcgt agttccagcc gtacttgatt
    24361 tgatcttcag tagtagatgc gccgccctgg atggttaacc cggcatcgcc gctaatagcg
    24421 gttgccagta attcagacag gagagatttt gcggtgccag gttcccccac caacatcaaa
    24481 ccgcgcccgg tggcaagagt gactaacata cgttcgacag cggagggact aatcaccact
    24541 ttacggctaa tattttgcgc gctatcgcca aggataaaag cacgggccgc aggcaaactt
    24601 aactgccaac cgggcgggca aggtgcggca tcattttgtt ttaattttgc cagttcatcg
    24661 gcgtataaca cagcagcagg cggacgctgc agatgattat tctgcggtga catatattca
    24721 tccctgaaat gtattaatga aataaacgat attttacgcg caataaatgt ttggcgcatt
    24781 agttgtttga gcgaaaaata tctggaaagc agatcacagt aaataaaaaa aactgctggt
    24841 gaggcaggtt cttttattta ctattttcag gcctaacacc acagcctgtg caacatcgtg
    24901 ttccaacgaa cgaattataa ccacggctgg accccgttac aattaagata atgagattta
    24961 taatccagac cgttgggacg ttgcgccgga tcgaacctat ttcaagtcct ggctaccgga
    25021 cgggtggtgc tgcaggcggt gccctgatcc ggagtgcaac atcgtggtcc aacatacgta
    25081 aaccagtatc gacaggcagt ttaagggtaa ggtgctggtg ttgcgctcct taataaggag
    25141 agcggatgga cacgctgaca caaaagttaa ccgtgctcat tgctgtactg gagctat
//
LOCUS       DAZGBC010000067        24776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGBC010000067 DAZGBC010000000
VERSION     DAZGBC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.067"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..531
                     /locus_tag="ACHK1J_002589"
     CDS             <1..531
                     /locus_tag="ACHK1J_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO7911839.1"
                     /translation="YDEQVNASWSEVLNQYQRRADLIPNLVASIKGYSSHERDVLEAV
                     TLARSQANRASSDLQQTPGDEQKLQAWQQAQAQVTRTLGQLTIISERYPELKSQELYQ
                     NLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYTPKKNYLPDDVAA
                     VSKAPTIDFSQNANAH"
     gene            545..1216
                     /locus_tag="ACHK1J_002590"
                     /pseudo
     CDS             545..1216
                     /locus_tag="ACHK1J_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            1453..2601
                     /locus_tag="ACHK1J_002591"
     CDS             1453..2601
                     /locus_tag="ACHK1J_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO7911840.1"
                     /translation="MRIFLLLLMLCSFQVFAERLPSVNDLTGTLTTEEQSVLNQQLQT
                     LEQQNHLQVAVLVTPTTGGKNIKLAASQSYGLYHWKPIGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPKQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASIKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            2598..3500
                     /locus_tag="ACHK1J_002592"
     CDS             2598..3500
                     /locus_tag="ACHK1J_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO7911841.1"
                     /translation="MIVMRLIFILLALWSLPGLAQQIAVPELRQQVTDITGTLRTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     GGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3560..4858)
                     /gene="eno"
                     /locus_tag="ACHK1J_002593"
     CDS             complement(3560..4858)
                     /gene="eno"
                     /locus_tag="ACHK1J_002593"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO7911842.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4946..6583)
                     /gene="pyrG"
                     /locus_tag="ACHK1J_002594"
     CDS             complement(4946..6583)
                     /gene="pyrG"
                     /locus_tag="ACHK1J_002594"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO7911843.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6811..7602)
                     /gene="mazG"
                     /locus_tag="ACHK1J_002595"
     CDS             complement(6811..7602)
                     /gene="mazG"
                     /locus_tag="ACHK1J_002595"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO7911844.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7673..8008)
                     /gene="mazF"
                     /locus_tag="ACHK1J_002596"
     CDS             complement(7673..8008)
                     /gene="mazF"
                     /locus_tag="ACHK1J_002596"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO7911845.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8008..8256)
                     /gene="mazE"
                     /locus_tag="ACHK1J_002597"
     CDS             complement(8008..8256)
                     /gene="mazE"
                     /locus_tag="ACHK1J_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO7911846.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNGITPENLHENIDWGEPKDKEVW"
     gene            complement(8334..10568)
                     /gene="relA"
                     /locus_tag="ACHK1J_002598"
     CDS             complement(8334..10568)
                     /gene="relA"
                     /locus_tag="ACHK1J_002598"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO7911847.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10616..11917)
                     /gene="rlmD"
                     /locus_tag="ACHK1J_002599"
     CDS             complement(10616..11917)
                     /gene="rlmD"
                     /locus_tag="ACHK1J_002599"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO7911848.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYSRAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            11974..14730
                     /gene="barA"
                     /locus_tag="ACHK1J_002600"
     CDS             11974..14730
                     /gene="barA"
                     /locus_tag="ACHK1J_002600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO7911849.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14963..16303)
                     /gene="gudD"
                     /locus_tag="ACHK1J_002601"
     CDS             complement(14963..16303)
                     /gene="gudD"
                     /locus_tag="ACHK1J_002601"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO7911850.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16324..17664)
                     /gene="gudX"
                     /locus_tag="ACHK1J_002602"
     CDS             complement(16324..17664)
                     /gene="gudX"
                     /locus_tag="ACHK1J_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO7911851.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17666..19018)
                     /gene="gudP"
                     /locus_tag="ACHK1J_002603"
     CDS             complement(17666..19018)
                     /gene="gudP"
                     /locus_tag="ACHK1J_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO7911852.1"
                     /translation="MSSLSQAASRVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(19453..19902)
                     /gene="yqcA"
                     /locus_tag="ACHK1J_002604"
     CDS             complement(19453..19902)
                     /gene="yqcA"
                     /locus_tag="ACHK1J_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO7911853.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19920..20702)
                     /gene="truC"
                     /locus_tag="ACHK1J_002605"
     CDS             complement(19920..20702)
                     /gene="truC"
                     /locus_tag="ACHK1J_002605"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO7911854.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20702..21031)
                     /gene="yqcC"
                     /locus_tag="ACHK1J_002606"
     CDS             complement(20702..21031)
                     /gene="yqcC"
                     /locus_tag="ACHK1J_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO7911855.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDVPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21653..22198)
                     /gene="syd"
                     /locus_tag="ACHK1J_002607"
     CDS             complement(21653..22198)
                     /gene="syd"
                     /locus_tag="ACHK1J_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO7911856.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            22266..23114
                     /gene="queF"
                     /locus_tag="ACHK1J_002608"
     CDS             22266..23114
                     /gene="queF"
                     /locus_tag="ACHK1J_002608"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO7911857.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGADIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGNVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23226..24590
                     /gene="ppnN"
                     /locus_tag="ACHK1J_002609"
     CDS             23226..24590
                     /gene="ppnN"
                     /locus_tag="ACHK1J_002609"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO7911858.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKHTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6098 a         6510 c         5938 g         6230 t
ORIGIN      
        1 tatgatgagc aggttaacgc ctcctggtct gaggtattga atcaatatca acgtcgcgcc
       61 gatcttattc ctaatcttgt cgccagcatt aaaggttatt ccagccatga acgagatgta
      121 ctggaagccg taacattggc tcgtagccag gctaatcgcg ccagcagcga tcttcagcaa
      181 actccgggag atgaacaaaa attacaagcc tggcaacagg ctcaagcgca ggtgactcgc
      241 actctcggtc agttaacgat aattagtgag cgttaccctg agttaaaatc tcaggaactc
      301 tatcaaaatt taatggtaca gcttgaagga agtgaaaatc ggattgccgt tgcgcgagga
      361 agatatatta aagccattga acaatataat gtcactatcc gcaaattccc cgctgtgtta
      421 accgcaaagg tcatggatta cacaccaaag aaaaattatt tgccggatga tgtcgccgca
      481 gtaagtaaag ccccaacaat agattttagc cagaatgcta atgctcatta agcggggaag
      541 ttctatgcga aattttatct tccttatggc tttcttctgt tcatctgtgt ttgccacaca
      601 aattccagta cctgaatcac ccaagtatgt gaatgactta acgggcacat taacaaacag
      661 cgaagtaaac accctgacca accagattaa agccctgacg caaaaaagcc acgcacaact
      721 ggtcgtgtta ttggttgaaa caacaggcga tgaaaccatc gagcagtacg caacgcgggt
      781 attcgatagc tggaaaccgg gtgataaaga ccgcgatgat ggcgttctac tgttagtcgc
      841 ctggcaggat cataccgtgc gtattgaaat cggttacgga ctggaaggta ttattacgga
      901 tgcgcagtcc gggaaaatta ttcgcaacag tattattcct gcatttaaaa aaggcgacct
      961 ggctggcggt ttacaaaagg ggatcagcga tattgaaagt cgtcttacgg ggaataattt
     1021 agcgacgata gcaccggccg atcacccttt accttttagc ggttggtggg ccttgttagc
     1081 ctgggcgata gtactgacgt ttatttctgc cagaggatat atcaaaacgc tgggagtaat
     1141 ttgcttcgca gctattgtac tggcctttgt tttacctata gcaggtttca gcggaagctg
     1201 gggagtactc acaacattac tctgcttcgc cacaccgttc ctggctgtcg ccatcgcttt
     1261 tacccccttc ggcaaaaaag ttcgggattt gatgcgtgat gccaaccagc catcacgcca
     1321 tacaagaagc aattcttcct cccactattc atcatcgtca tcctcttcat tcaacaatga
     1381 caatttcagc ggtggtggcg gttcatctgg tggcggtggc gcttccgggc gctggtaaca
     1441 aaaggaattg atatgcgtat ttttctgtta cttctgatgc tatgcagttt tcaggtattc
     1501 gctgaacgat tgccttcagt gaatgactta accgggacat taacgacaga agaacaaagt
     1561 gttttaaatc aacaattaca gactctcgag cagcaaaacc acttacaggt ggccgtgtta
     1621 gttacgccaa ccacaggggg caaaaacatt aaacttgcag cctcgcagag ctacgggtta
     1681 tatcactgga aacccattgg agagaagcga tacggcgaag gtatcttaat tctcgtcgtt
     1741 tggccggaag ggctggcgtc aatgaagatt ggacatggac tggaagagat gttgccgccg
     1801 aaacaagctg cgcaaattgt tcgttaccat atgcagccag aattcgaaaa aaacaatctc
     1861 tttgctggat taacgggcgg aattgaaagc atcgcacagt ttacccatat caaagcgaac
     1921 cttagccctt tggatgcttt agccaatcat ctgtttgcta atccgcaacg ttcgctgcca
     1981 tgtcttgcct ggacactgct gatgatcgtt gccatcgttg tcctctggcg atttgccagc
     2041 cgcccacggc gtggcatatg gatgatatcg atgattaccc ctatggtctg gtttttctcc
     2101 tttcaggacg atgtcatcat tcggcgggta tcggtcgttt gttttagttt attgttagca
     2161 acaacctgca agcagcgtct cgcggtagtt tttaatatgt gccgcgtatt tcctatgatg
     2221 ctggccccaa aaaataaaaa cgctaaagtg aaaaaacaaa agaagactcc gcaggttcgt
     2281 gaatcaggac gaaattcatt gtttgtcgtg atgctgataa ttattgtggg atggtgcctg
     2341 gtgttcgcca gtattaaaga tattgcattc atctcaacaa ttattgggtt aattgatcat
     2401 tttatcggtc caataaccat tgctggcatc gttctgctga tcattgtggc caaatttact
     2461 ggcaatctta agctgggatc aggagaaaaa agcaacagga aaacggggaa tcgtaatgct
     2521 cactctcgct cttcgtcgag aaattctttc cgcggtggtg gcggttcttc aggaggcggc
     2581 ggttcatccg gccgttggtg atcgtaatgc gcttaatctt tattctactt gctttgtgga
     2641 gccttcccgg gcttgcccag caaattgctg tgccggagct gcgccaacaa gtgactgata
     2701 ttactggtac gttaagaacg tctgaacaac aatcactgac acagcagctg caggatatta
     2761 cacataaaac acgggcgcag gtcgctgtat tagtagtgcc gtccaccgga gacgattcca
     2821 ttgaacaata tgctacaagg gtttttgata gctggaaact gggagataag cagagaaacg
     2881 atggtatttt acttctggtt gcctgggagg atcatgctgt tcgtattgag gttggctacg
     2941 ggctggaagg ggtggttacc gacctgcaag cggcgaagat tatcagggat atattgatcc
     3001 ctgccttcaa aagtgatgac ctgatgggtg gattaacact ggcaagcgaa aatatcggcg
     3061 cgcttctgtt gaatggtgaa ttaccggaag acagggggga ttattacagt atcaatcccc
     3121 ctattccatt atcgcttgct gtaattatat tgctggcggt actttcatat tttattgttt
     3181 ttaccgatcc gtcgaattta ccctggatca ctctcactgg cgccatttac gggatggtat
     3241 tcctctatgt tgccgagcca ggaccatgga ccaatttaat cgttgcctgc ggtatgttaa
     3301 cgccttttgc gatcgtacca ctggttattt tttggcttat cgtcaataaa aagctacgcg
     3361 ccaaatacaa aaaactcagt aaagaaagag cttcaagaaa aggttcttct tcatcctctt
     3421 ccggtggagg ctcaagtggc ggcggattta gcggcggggg cggttcttct ggaggtggcg
     3481 gcgcatccgg ccgctggtaa ctcaaccata aaaaatgcca gcctccgggc tggcattttt
     3541 aaatcagata aagtcagact tatgcctggc ctttgatctc tttacgaccg ttgtacggtg
     3601 ctttttcgcc cagagcttct tcgatacgaa tcagctggtt gtatttagca acgcggtcag
     3661 aacggctcat agaaccagtt ttgatctggc ctgcagcagt accaacagcc aggtcagcga
     3721 tggtagcgtc ttcagtttcg ccagaacggt gagagataac tgcagtgtag ccagcgtctt
     3781 tcgccatctt gattgcagcc agagtttcgg tcagagaacc gatctggttg aatttgatca
     3841 ggatggagtt agcgatacct ttttcgatac cttctttcag gatcttggtg ttggttacga
     3901 acaggtcgtc accaaccagc tggattttgt cgcccagaac tttggtctgg tatgcgaaac
     3961 cgtcccagtc agattcgtcc agaccgtctt cgatagaaac gatcgggtac tgtttggtca
     4021 gttcttccag gaagtgagtg aattcttcag aggtgaaagc tttgttgcct tcgccagcca
     4081 gaacgtattt accgtctttg tagaattcag aagccgcgca gtccatcgcc agagtgatgt
     4141 ctttgcccag ttcataacca gcagctttaa cagcttcagc gataacagcc agagcttcag
     4201 cgttggaacc caggttcggc gcatagccac cttcgtcacc aactgcagtg ttcatgcctt
     4261 tcgctttcag aacttttgcc agatgatgga aaacttcaga acccatgcgg atggcttctt
     4321 tcacagtttt cgcgccaacc ggctgaatca tgaattcctg gatatcgacg ttgttgtcag
     4381 cgtgctcacc accgttgatg atgttcatca tcggaaccgg catggagtat ttgcccggag
     4441 tgccgttcag ttcagcgatg tgctcgtaca gcggcatacc tttagcagct gcagcagctt
     4501 tggcgttagc cagagataca gccaggattg cgttcgcgcc gaatttggat ttgttttcgg
     4561 tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg atctttagca tctttgccaa
     4621 tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc tttggttacg cctttaccca
     4681 ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc acgggaacca gtagaagcac
     4741 ctgacggagc agctgccata ccgacgaaac caccctccag atgtacttcg gcttcaacag
     4801 tcgggttacc acgggagtcg atgatttcac gaccgatgat ttttacgatt ttggacatta
     4861 ggttttcctc aagtcactag ttaaactgaa actccagaca aacaacgcgt acccagggta
     4921 cgcgttgccg ttctaacttt tttacttact tcgcctgacg tttctggaac tcgctggcgg
     4981 ctttcacaaa gcctgcaaac agcgggtgac catcacgtgg agtagaagta aactccggat
     5041 ggaactggca agccacgaac cacgggtgat tcggaacttc gatgatctcg accaactgat
     5101 catccccgga acggcccgca acgcgcagac ctgcatcttc aatctgtttc aacagcatgt
     5161 tgttgacttc gtaacggtga cgatgacgct caacaattgt cggcgcattg tacagctggc
     5221 gaaccaagct atcgtcaacc aactggcact gctgtgcgcc gagacgcatg gtaccgccga
     5281 gatcgctctt ctcgctacga acttcaacgt tgccgttttc atcgcgccac tcggtaatca
     5341 gcgccacaac cgggtactta cagtctggca caaattccgt agagttggcg ttctccatgt
     5401 tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat acccaggcaa atgcccagat
     5461 aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat catgccttct acgccacgat
     5521 agccgaaacc gccaggtacg aggattgcgt ccagaccttt aaggatttca acgccgcgcg
     5581 tttcaacatc ttgtgaatcg atcagtttga tgttgacgct gacacgattc ttcagcccac
     5641 cgtgtttcag tgcttcgatc acggatttat aagcatccgg cagttcaatg tacttgccga
     5701 ccataccgat ggtgacttca cttaccggat tcgcttcttc gaagataacc tgttcccatt
     5761 cggacagatt cgcttccggg cagtttaagc tgaatcgttt acaaatataa tcgtccagcc
     5821 cctgagattt caacaggccc ggaattttat agatggaatc gacgtctttc agagaaataa
     5881 ccgctttttc cggaacatta cagaacaatg caatcttcgc acgttcgttc gccggaacgg
     5941 cgcgatctga acgacaaatc aggatgtcag gctggatacc gatggagagc agctctttta
     6001 cagagtgctg agtcggtttg gttttgactt caccagacgc tgccatgtac ggcaccagcg
     6061 tcaggtgcat aaacagagtg tgctcacggc caatttcaac agccatctgg cgaatcgctt
     6121 cgaggaacgg cagggattcg atatcaccta ctgtaccgcc gatttcgacc agtactacgt
     6181 catgaccttc gccaccttcc agcacgcgct ctttgattgc gttagtgatg tgcggaataa
     6241 cctgtacggt tgcgccgagg tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga
     6301 tacgacccgt ggtgaagttg ttgcggcggc tcattttggt acggatgaaa cgctcgtagt
     6361 gccccaggtc caggtcggtt tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga
     6421 ttgggctcat agtacctgga tcgacgttga tgtacggatc cagtttcatg atggtcacat
     6481 tgaggccacg ggcttcaaga atggctgcga gggaggctgc ggcaatgcct ttacccagag
     6541 aggatacgac cccgccggtc acaaaaatat agttcgttgt catgctgaac ctgagaagtt
     6601 aggttgaaaa gacgatggaa taaccaggac gggaaagcag tatacccgaa catgacctgt
     6661 gccacaaact ttcattatcc ctcctcttcg ccagcgcact attgaaatca ggagtgagaa
     6721 aatagcccct ttggggtaaa tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg
     6781 acacaaatgg cgcttgaccg cgtaattccc ttagagatca atttcctgcc gttttacctg
     6841 ttgccagact tcttccattg tttcgaggtc aacacctgtc atttccagtc cacgcgcggc
     6901 aacaatacgc tccacttcgc gaaaacgacg ctcgaatttt tcgttcgctt tttgcaatgc
     6961 gatttccgct tttgtcccta aatgacgagc cagattgacc gtggcaaaca gcaggtcacc
     7021 catttcctcc tccagtttag cctggtcgat aacagcctgc cgtgcctcgt acatcacctc
     7081 gtcgatctct tcgtagactt tatcaaccac tgggccaagc gtcgtccagt cgaagccaac
     7141 gttggcgcaa cgtttctgga ttttttgcgc ccgcattaaa gccggtaaac tacgaggaat
     7201 atcgtccagc gccgaatgct gcgctttctg cgcgcgctct tcggttttga tttgctccca
     7261 acgggcaagc acttcactac tgttttcggc agaactgtca gcaaaaacat gcggatggcg
     7321 acgctctaat ttatcgctaa tcgcagcgca aatatcatta aagtcaaagc gcccttcttc
     7381 ctgagccatt tgggcgtaaa acaccacctg gaacagcaga tcgccgagtt caccacgcag
     7441 atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg taggtttctt caagggtgta
     7501 aggcgcaatg gtggcaaatg tctgctcttt atcccacggg cagccgtttt ccgggtcgcg
     7561 caggcgctgc ataatagtga gcaaacggtc gatttgattc attagaatgt cctgaaaatt
     7621 gcgggtccgt caggtggaaa cctgtgacca gaatagaagt gagttagtaa cactacccaa
     7681 tcagtacgtt aattttggct ttaatgagtt gtaattcctc tggggcaact gttcctttct
     7741 tcgttgctcc tcttgcccgc caggcgatac tttttacctg atcagctaac gctacgccat
     7801 cacgttcctg accggataaa acaacttcga acggatatcc ttttgattgc gttgtacaag
     7861 gaacacacag acacatacct gttttgttgt tgtacatgaa cggactcagg acaacagccg
     7921 gacgatgtcc ggcttgctcg ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc
     7981 ccatatcggg tacgtatcgg cttaccatta ccagacttcc ttatctttcg gctctcccca
     8041 gtcgatattc tcgtggaggt tttccggcgt gatgccgttg accagttcag caagcgtaaa
     8101 tacgggctct ttacgcactg gctcaataat taatttgcca tccaccaggt caatcttcac
     8161 ttcatcatca atattcagat tgagcgcctg cattaacgta gccgggatcc gcaccgccgg
     8221 tgaatttccc caacgcttta cgctactgtg gatcataacc ctttcctcaa accgctatca
     8281 tatgtagata cagtatatat caatctacat tgtagatacg agcaaatttc ggcctaactc
     8341 ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt tgagtttacc cagcacgcga
     8401 cccagcacct gcaggttgta aatctcaatg gtcatgtcga tggtcgccag ttgctgtttg
     8461 gtgtcgctac ggctggcaac gccaagcacg ttcaccttct cgttggcgag aatggtcgtg
     8521 atatcacgta acaacccact acgatcatta gccaccacgc ggaccaccag cgaatatccg
     8581 gcggagtagc tctcacccca taccgcgtca acaatgcgtt ctggcgcatg ggagcgcagt
     8641 tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac cgcgcccctg ggttatgaag
     8701 ccgacaatct catctccagg aatcggctgg cagcagcgcg cgatgtggtg catcaggtta
     8761 ccgacacctt caaccaccac acgaccgtta tctttactgc ggttttgcgg tgtgtagctt
     8821 ttttgctgaa gctgtttcag cgcggcggcg tcctgctctt cggcactcgg cttattaaat
     8881 tgcgattgca ggaagttcac catctgattg agacggatat ccccgccacc aatcgccgcc
     8941 agcaactcgt cgacatcatt gaagttgtaa cgcggcagca gatgtttttc tgcttctttc
     9001 aggctgatcc ccagatgttc cagctcgtcg tcaaggattt gccgcccagc cagaatgttt
     9061 ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg aacgcccacg gctggttgtg
     9121 acgtaaccga ggtttgggtt taaccagtca cggctggggt tcggctgttt ctgggtgata
     9181 atttcaatct ggtcgcccat ttgcagctgg taggtgaacg gcacaatgcg cccgccaatt
     9241 tttgccccga tacagcggtg tccgacatca ctgtggatgt ggtaagcgaa gtccagcggc
     9301 gttgatcccg caggcaaatc aacgacatca cctttcggcg taaagacata cacccggtcg
     9361 tcaaacacct gactacgtac ttcgtcgagc atttcgccgg aatcagccat ctcttcctgc
     9421 cacgcaatca gtttacgcag ccaggcaatc cggtcttcat gtcccgaacg tgcgccgcca
     9481 gctgccgcgc cctctttata tttccagtgc gcagcaacac ccaactctgc atcttcatgc
     9541 atctgtttgg tgcggatttg gatctcaacg gtttttccac ccggccccag aaccacggta
     9601 tgaatagact gataaccgtt tggtttcggg ttagcgacgt aatcatcaaa ctcatccggc
     9661 aggtggcgat agtgtgtgtg cactatcccc agtgcggcat aacaatcctg taaacgctcg
     9721 gcgacaatac gtaccgcacg cacatcaaac agctcatcga aggcgaggtt ctttttctgc
     9781 attttgcgcc agatgctgta gatgtgtttc ggacgaccat acacttcagc tttaacgcct
     9841 tcagctttca tctcagcgcg cagatgaccg acaaactctt cgatatagtg ttcgcggtcg
     9901 agacggcgtt catgcaacag ttttgcgatg cgtttgtatt cggtcgggtg gagataacgg
     9961 aagcagtaat cttccagctc ccatttcagt tgcccaatcc caagacggtt cgccaacggc
    10021 gcgtagatat tggtgcactc ttttgccgcc agtacgcgtt catcttccgg cgcatctttt
    10081 acttcacgca gatgagcaat acgctccgcc agtttgatga ccacgcagcg aaaatcatcg
    10141 accatcgcca ataacatccg gcgaacgtta tcgacctgtt cggaggaaac agaatcagtg
    10201 tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca cgccgtgaat aaggttaacg
    10261 accgacttac cgacgctctc acgcagcaca tcttcgctga ctacgttggc atccgccaga
    10321 gggaaaagca gcgccgcccg cagcgtgtca atgtccatac ttaatgtcga gaggatctcc
    10381 accatctcaa caccacgcca caataacaga ctggcatccg gatgcccctg cgtctgttgc
    10441 agacaatacg cccaggtttc ggctaagcac tcacacgact tctggctggt aatacccaga
    10501 cttgcgatcc atttttccgg atcaaattca ccagccttat tgatatgtgc acttcttacc
    10561 gcaaccatcg tcctctcctt tagggaccag acctgccgaa atcggcaaat cgcaactatt
    10621 taacgcgcga gaaaagtacc atcgattcca gatgtcccgt gtgtgggaac atatccagca
    10681 tcgccagtcg cgcaatggta tatcctgctt ttaataacgc ttcgctatcc cgagccagcg
    10741 ttgcagggtt acaggataca taaactatac gaataggttc cagttttata atttgctgca
    10801 taacacctgc ggcacctgct cgcgcggggt ccagcaacac tttatcgaag ccgtttttcg
    10861 cccacggctg ctttgtgaca tcttcttcaa gattttcatg ataaaacgtc acattctgta
    10921 agccattaag acgcgcattc tgctggcctt tttccaccag cgcaggaaca ccttctacac
    10981 ccaccacact ggctgcttgt gtcgccaatg gcagtgtaaa gttgcccata ccgcagaaca
    11041 gatccagtac gcgatcttca ggttgcacat ccagccattc caacgcacgc gctaccattt
    11101 tttggttcac acccgcattg acctgaataa aatcgcgcgg gctaaaagtt aagcgcaacc
    11161 catttgagtc ataccagggc atctcaccag agacggtttc gagtatctca ctatcggggg
    11221 cgagatacag atccaggcct tcagaatgcg aaaagcgttc cagtttttcg cgatctgccg
    11281 aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc gctggttgcc tgtaccagtt
    11341 caacatgacc aagatggcgc atagcttgta agctgcccag gcatgccctg actttgggca
    11401 gcaatgcttc aagttggggc actaaaatgg ggcattgttt aacgtcgaca atgtcactgg
    11461 agcccgcttt gcgaaacccc atctgaagtt gctgtgtttt cggtaagtaa ttcagactta
    11521 aacgcgcgcg acggcgatag ccccagggaa catcggcgat cacttcagag acttcgtgtt
    11581 tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg taaatccacg ctggcgtgtt
    11641 gttgctgaca gccgccgcat acgccaaaat gaggacagcg tggcgtttcg cgttccgggc
    11701 tatcgcttaa ccggcgtacg actttagcgc gggaatactg ttttttatct tcagtaacag
    11761 taacttccgc gttttcctgc ggcaataatc cggggataaa tagcgttttg ccgttatgtc
    11821 gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga aacggttatg atctgacgcg
    11881 tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg cgagactttc tcaatttaac
    11941 agtgtgacct taattgtccc ataacggaac tccatgacca actacagcct gcgcgcacgc
    12001 atgatgattc tgatcctggc accgaccgtc cttattggtt tattgctgag tatctttttc
    12061 gtcgtgcatc gctataacga cttgcagcgt caactggaag atgccggtgc cagcattatt
    12121 gagccgcttg cagtttctac tgaatatggc atgagcctgc aaaatcgcga atctatcggt
    12181 cagttaataa gcgtactgca tcgtcgccat tccgatattg ttcgcgcgat ttcggtttat
    12241 gatgaaaata accgactctt tgtcacctcc aattttcatc ttgatccctc atcaatgcag
    12301 ctcggcagca acgtgccgtt tcctcgccag ctcactgtca ctcgtgacgg cgatattatg
    12361 atcctccgca cgccgattat ttctgagagt tactcccctg acgaatcgcc cagtagcgat
    12421 gccaaaaata gtcaaaatat gttgggatat attgcgcttg agctggatct taaatcggtt
    12481 cgcttgcagc aatataaaga gatctttatt tccagcgtga tgatgctgtt ttgtatcggt
    12541 attgcgctta tttttggctg gcgcttaatg cgcgatgtaa ccggtccgat tcgcaacatg
    12601 gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca gccgggtgga aggatttatg
    12661 ctcggcgagc tggatatgct gaaaaacggt atcaactcga tggcaatgtc gctggctgct
    12721 tatcacgaag agatgcagca caatattgac caggcgacgt ccgatctgcg tgaaacgctg
    12781 gagcagatgg aaattcagaa cgttgagttg gatctggcga aaaagcgcgc ccaggaagcg
    12841 gcgcgtatta aatccgagtt tctggctaat atgtcacacg agctgcgtac accactgaat
    12901 ggtgttattg gctttacccg cctgacgctg aaaacagaat taacaccaac gcagcgcgat
    12961 cacctgaata cgattgaacg ttcggcaaat aatttgctgg caataattaa tgatgttctc
    13021 gacttctcaa aactggaagc aggtaagctg attctggaaa gtattccatt cccactacgc
    13081 agcacgctgg atgaagtcgt taccctgctg gctcattctt ctcacgataa agggctggaa
    13141 ctgacgctca atattaagag cgacgtgcct gataacgtga tcggcgaccc actgcgatta
    13201 cagcaaatca tcactaacct ggtggggaat gcaattaaat tcaccgagaa tggcaacatt
    13261 gatattctgg tagaaaaacg tgcgctgagt aataccaaag tgcagattga agtgcagatt
    13321 cgggataccg gcattggtat tcctgaacgc gatcaatcgc gcttattcca ggccttccga
    13381 caggctgatg ccagtatttc ccgccgtcat ggtggcaccg ggctggggct ggtgattaca
    13441 caaaaactgg ttaatgaaat gggcggcgat atttcgttcc atagccagcc aaatcgcggt
    13501 tcaactttct ggttccacat taatctcgat ctgaacccga acattattat tgaagggcca
    13561 tccacccagt gcctcgccgg taaacgcctg gcctatgtcg aaccaaactc cgcagcagcg
    13621 caatgcacgc tggatatttt aagtgaaacg ccgctggaag tggtttatag cccaacgttc
    13681 tccgcgctgc ctcccgcgca ttacgacatg atgttgttag gcatcgcggt gaccttccgc
    13741 gagccgttaa caatgcaaca tgagcgatta gcgaaagcgg tatcgatgac cgatttcctg
    13801 atgctggcac ttccttgcca tgcacaagtc aatgctgaaa aactcaagca agatggtatc
    13861 ggcgcgtgtc tgctgaaacc attaacacct acgcgcctgc tgcctgccct gacggaattt
    13921 tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg aaagtaagct ggcaatgaca
    13981 gtcatggcgg ttgatgacaa ccccgctaac ctgaaactta tcggcgcatt gctggaagat
    14041 atggtgcaac atgtggaact ttgcgatagc gggcatcagg cggttgaacg ggcgaaacag
    14101 atgccgttcg atttgatctt aatggatatt caaatgccgg acatggatgg cattcgggcc
    14161 tgcgagctca tccaccagct cccccatcag caacaaacgc cggttatcgc ggtaacggcg
    14221 catgcaatgg ccgggcaaaa agagaagctg cttggcgcag gaatgagcga ttatctggcg
    14281 aaaccgattg aagaagagcg attgcataat ttgttgttgc gctacaagcc tggcagcggt
    14341 atttcgtctc gcgtcgtgac gcccgaagtc aacgaaattg tggtgaaccc gaatgcgacc
    14401 ctcgactggc aactggcact acgccaggca gcaggaaaaa ccgatttagc gcgcgatatg
    14461 ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca aagttgagga acagctggtt
    14521 ggagaaaacc cggaaggcct ggttgatttg attcataaac tgcatggcag ttgcggctat
    14581 agcggtgtgc cgcgtatgaa gaatctctgc caactgatcg aacaacagct acgtagcggt
    14641 actaaagaag aagatttgga gccggagctg ctggaactgt tggacgagat ggataatgtc
    14701 gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt gtcgagtagc aatcggcttt
    14761 ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt ttatgccgga tgcggcgtta
    14821 acgccttatc cggcctacaa aaccaattaa attcaataaa ttaaaaagca tgtaggcctg
    14881 ataagcgtag cgcatcaggc aattttgtgt ttggcatcag tctcaaaccg gctcctgaca
    14941 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    15001 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    15061 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    15121 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    15181 tgccttcctg ccaaatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    15241 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gttagagtgt gaaccccagg
    15301 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcatc gtccagaaat
    15361 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    15421 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    15481 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    15541 aacctttcag gtatttacca attttaatcg cttcgttcag cgaccaggca ccgttaggat
    15601 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcctctt
    15661 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaaaccatat ttttcatacg
    15721 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    15781 accagtcgca tgagtcatcc ggctggcttt gatacggcag cggtgtggct ttgcgattac
    15841 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    15901 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    15961 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccgccggcat
    16021 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    16081 tacccactac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    16141 tttcccctac gccagtgtga ccagaattat ctttgataat cacaatatta cgcgtgaaga
    16201 acggtgcgtg tgcaccactc agattcatca gcatactgtc atggcccgcc accgggatga
    16261 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    16321 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    16381 ctgcatcggg cctgcgtcgt tacgcgcacc gccaggcaga cgtttatagg cctcatgtgc
    16441 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    16501 ttttccgttt ttaatctcca gcggattttg ggtcaggcga caatcgccct cctgccaaat
    16561 ccagtgggta tcgatagcgg taggattacc cggcgccgcc gcgcccacat gggtaaacat
    16621 cgccagagag atatcgaaat ggttattaga atggcagccc caggtcagcc cccagtcgtc
    16681 gcaaagctgc gccacacgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    16741 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    16801 gttagtcgcg acgggcaagc cggtcgcccg ccgaaactcc gccatcactt cacgtccgga
    16861 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    16921 gcacagagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    16981 atccgggaag cgtttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    17041 gccgccctta agtttaaaat ctttaaagcc gtagcgatcc tgtgaggctt ccgccagacg
    17101 cacaacggct tcgctgttca tcgctttctg atgccgcaac tgataccact catggttgcc
    17161 cggcgtattt tccagataag gaagatcggt tttggtccga tcaccgatat aaaacagata
    17221 accgaggacg gtaatagcct cgcgttgctt gcctggccct aacagttcgc agaccggaac
    17281 attcagcgcc ttacctaaca ggtcaagcaa ggcggcttcc agcgccgcta cggcattaac
    17341 gcgcaattca aaagtccagg cacctttgcc gaaggtatca aaatcggctg cttgattacc
    17401 tttatgcacc tgctgaacca ctttattcag gcgcgcgact tcctggccca gcaccatcgg
    17461 aatagcttcg acaagcgtct gataaatcac ctctccgccc ggcgcttcac caatgccggt
    17521 atgcccggcg ttatcggtga gtaccacaat attgcgggtg aaatatgcgt tatgtgcccc
    17581 accaatatta agcaacatgc tatcgtgccc ggcgaccgga atgactttca tatcagtaat
    17641 aacaggactg gattgtgtcg tcatcttatt gccccgcgac aggtttcagc tcgatacgct
    17701 tgatatcgcc caccagcacc aggtagctca gtaccgcgat taaggcatga acaccaacat
    17761 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    17821 tgacgatgcc agaaatgtta ccgaacatgt tgaacaggcc accggaaaga ccgctgatct
    17881 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    17941 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    18001 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgacc
    18061 ccgtgcggcg catcagccag tcggaaatta tcccacccag cacaccgccg ataaaaccgc
    18121 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatcccg cgcgcctgca
    18181 ccagataaac cgggaaccag gtaatgaaga agtaagtcag ggcgttgata cagtactgac
    18241 cgatataaac gccgatcatc atccgtgatc cgagcagctg tttgatctgc ccccacttca
    18301 cgctgaacgg cactttaact ttggtgtttt gctgatccat attgatcagc gcgccacccg
    18361 cggcgatgta ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    18421 ctttcaacca gataaagctg atgacaatcc ccagaccgcc cataaagaag aagacgtgtg
    18481 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    18541 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    18601 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    18661 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    18721 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    18781 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    18841 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    18901 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    18961 agtaacgagc attcgtgcgt ttttccacac ggctcgcagc ctgacttaaa gaactcattg
    19021 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    19081 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    19141 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    19201 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    19261 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtta aaacgaagaa ataaagaaga
    19321 gtatgaaatg gatcgcttga ctccaggcaa acgccagtaa aaatccgcgc tatgaagcag
    19381 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    19441 tcgctgaaca tatcaggaca acagcgtgcc ccactgttcg acccacggat ttgattccgt
    19501 ttccggttcc gggttctcac tggcgtcaat caacagcatt tcgccaaccc gctgagcgct
    19561 ctgttcctgc aacaaggcat cgaactgttt gccgccattg cagaaattta cataactgct
    19621 gtcgccgagc gcaatcacgc cataacgcag attcggctgg aagcccagac tatctttgat
    19681 tccctgaaag agcggcacaa tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac
    19741 caccagaaca tacttatcct gatagggcag ccagtcgctt aattcaggat cttcaaatac
    19801 cgttgctttg tggccctgtg cggtcagaat tgcttctgct tcttccgcca ctaacagcga
    19861 atttccgtac atggtgccga caaaaatacc aatttccgcc ataccgtttt ctccctggat
    19921 taggaactta tctctccatc ctgacctgat ggcgcactga actcaaccct ttcattttca
    19981 ggaagcagac cgcgccagcc aaattgtgat aacgcctgca tccaggtgtc gtccaggccc
    20041 gcgtgaatag tcagcggctc gccagtaaaa ggatgcgtca atgacaactg actggcatgc
    20101 agcattaacc gctgaagacc aaaatgctca gcaccgctgc gattctggcg taaatcgcca
    20161 tgtttgctat cgccaataat cggatgacgc aaatgggcaa gatgtcggcg aagctgatgt
    20221 ttgcgtccgg ttttcggctc cagttccacc aggccgtagc gcgtggtcgg gtaacgtccg
    20281 gtcgctaccg gcatttctac ggtcgccaga ccgcgataat gcgtcactgc tggctgcggg
    20341 cctttatctt cgcgggcaaa tttatcagcg attttgtcca gttcttccac cagtggataa
    20401 tccagcaccg cttcttccat caaccagccg cgcacaatcg catggtaacg tttctggatt
    20461 tggtgctgtt caaactgttg tgccagcagc cgtccggcct cgctggataa tcccatcaac
    20521 aacacaccag aagtgggtcg gtccagacga tgagcagtaa aaacatgctg tcctatctgg
    20581 tcacgcacgg tttgcatgac cactactttc tcgtcgcgat ccagccagct gcggtgaacc
    20641 agccagccgg agggtttatt taccgcaacc agccattcat cctgatagag tatttccagc
    20701 attagctcgc atcatccgca aaaagagcat ccagtttttc caactcagcc agaataagcg
    20761 cgcgttgcgg atggtccgtc gccagcgcca tttcataata gggagcaacg gcaaaagcgc
    20821 ccggtaacgg ctggttgtta tttaacaaat cgtgcattcg cgggatcagc acccactgca
    20881 accactccag tggttccatg gtgtccataa agaacggttg ggtactatta aattgatgcg
    20941 gctggggtac atcgtttcgc cagtgctgat gttcacgcag taacgcttca agcgcctgca
    21001 actggagacg aacgcggtca tgagtggtca taaagcaacc tcaataagaa aaactgccgc
    21061 gaaggatagc aggaataaaa aaagggagca ctgtattcac agtgctcccg gttcgtttcg
    21121 cagcattcca gctacatttg ttgctccctg ctcatccttg acaacttttc ctctggcctt
    21181 gcggccaatc gttcatcctg aactattgct tcctgctcac accaccccga tgtgatactt
    21241 catcctgaag tgtccctggc cttcctgacc caccgaatca tcctgaccgg ttctcattct
    21301 ccatcctgga ggtgtccttt aacgcgtcct gcgtcatcct cttcgcttca tccagaagcc
    21361 tttccctgaa acaccatcct ggtgtgtcct tcagaagtgt catcatcctg atgttcactt
    21421 cgttgtctga ctccctgtcg acgaagatag aatcgtcttt ttcgcgaagt cttacaaggc
    21481 gcttacagac aatgccttaa ggaaaatttc atatgaaagt taggaattaa caagcaacat
    21541 gttgttttta aatgaaaata acgattctta tctttttaag tattcgctta tttcctggcg
    21601 atctcctaca gagtctgtaa gagatctctc acaaccagat ggggtatttg gattacagaa
    21661 gaggcttaag ttgattaagg aattccgcaa gatttgaggc taaatgcgtc cgtttacgtg
    21721 ttcccagcgt ctctttacac acttcaccgg agagattaca caccgagatg acctctaact
    21781 cttcttccag cgtcgcgata aatagcgtgg gtggcagttt tagccttttc tgagtcacca
    21841 gatgaccaat cagattttcc tgcacccgac ggaagtcgtc ttcactccag gtttgcagca
    21901 atgtcagttt gatatcgcca aactgcgcgt gcatatcccc ggcaaactga gtcgtataaa
    21961 aggtatgaat tgtgggttgt atcacaatat caaaagcgcg ttcaaccgcg tttacatttt
    22021 gttccccggt aaacggctgg ggttgccagt atacggcatc ttcagtagtt gaaataatgc
    22081 acggtgaagg aacgccgtac agttcctcac ttaacggcca gcttttatgc tcttcatgcc
    22141 acgcatcgca gtagcgtgca gtaaaatctt tcagggcttg tgcggtcaaa tcgtccacta
    22201 atttctctct tcacttaaac cagatacact tgtcgtttag tttatctggt ttatgacggt
    22261 gaaacatgtc ttcttatgca aaccatcagg cacttgcggg cctgactctt ggaaaatcaa
    22321 ccgattaccg ggatacctat gacgccagcc tactgcaagg cgttccacgc agcctgaatc
    22381 gcgatccgct gggtctgaaa gcggataacc tgccttttca aggtgcggac atctggacgc
    22441 tgtatgaact ttcctggctg aatgcgaaag gtttgccgca ggtcgctgtc ggtcatgttg
    22501 aacttgatta caccagcgta aatctgattg agtcgaagag ttttaagctc tatctcaaca
    22561 gttttaacca gacgcgtttt aataactggg atgaggtgcg ccagacgctg gagcgcgact
    22621 taagcacttg cgctcagggt aatgttagcg tggcgttata tcgtcttgat gaactggaag
    22681 gccagccgat aggtcatttc aatggcactt gcattgatga ccaggatatc actatcgata
    22741 actatgaatt cactactgac tatctggaga atgccacctg tggtgaaaaa gtagtggaag
    22801 agacgcttgt cagccacctg ctgaaatcaa actgcctgat cacccatcaa ccagattggg
    22861 gttcgatcca aattcagtat cgtggacgcc aaattgacag agaaaaactg ctgcgttacc
    22921 tggtctcatt ccgtcatcac aacgagttcc acgaacagtg cgtggaacgc atctttaatg
    22981 acctgttacg cttctgccag ccagagaaat tgagcgttta cgcacgttat acccgtcgtg
    23041 gcggtctgga cattaacccg tggcgcagta atagcgattt tgtcccatcg accacaagac
    23101 tggttcggca ataaattttt tctcaatttt gcgtgctgga ttcacgcaga aggttgtgaa
    23161 aggtcatcag gcagggctat tgtaatcaaa gggaatgacg atattcgtcc cataaggagt
    23221 ttttcttgat tacacatatt agcccgcttg gctccatgga tatgttgtcg cagctggaag
    23281 tggatatgct taaacacacc gccagcagcg acctctatca attgtttcgc aactgttcac
    23341 ttgccgtact gaactccggt agtttgaccg ataacagcaa agaattgctg tctcgttttg
    23401 aaaatttcga tattaacgtc ctgcgccgtg aacgcggcgt aaagctggaa ctgattaatc
    23461 ccccggaaga ggcttttgtc gatgggcgaa ttattcgcgc tttgcaggcc aacttgttcg
    23521 cggttctgcg agacattctc ttcgtttacg ggcaaatcca taacaccgtt cgttttccca
    23581 acctgaatct cgacaactcc gtccacatca ctaacctggt cttttccatc ttgcgtaacg
    23641 ctcgcgcgct gcatgtgggt gaagcgccaa atatggtggt ctgctggggc ggtcactcaa
    23701 ttaacgaaaa tgagtatttg tatgcccgtc gcgtcggaaa ccagctgggc ctgcgtgagc
    23761 tgaatatctg caccggctgt ggtccgggag cgatggaagc gccgatgaaa ggtgctgcgg
    23821 tcggacacgc gcagcagcgt tacaaagaca gtcgttttat tggtatgaca gagccgtcga
    23881 ttatcgccgc tgaaccgcct aacccgctgg tcaacgaatt gatcatcatg ccggatatcg
    23941 aaaaacgtct ggaagcgttt gtccgtatcg ctcacggtat cattatcttc cctggcggtg
    24001 tgggtacggc agaagagttg ctgtatttgc tgggaatttt aatgaacccg gccaacaaag
    24061 atcaggtttt accattgatc ctcaccggcc cgaaagagag cgccgactac ttccgcgtac
    24121 tggacgagtt tgtcgtacat acgctgggcg aaaacgcgcg ccgccattac cgcatcatca
    24181 ttgatgacgc cgctgaagtc gctcgtcaga tgaaaaaatc gatgccgctg gtgaaagaaa
    24241 atcgccgcga caccggtgac gcttacagtt ttaactggtc aatgcgcatt gcgccagatt
    24301 tgcaaatgcc gtttgagccg tctcacgaga atatggctaa tctgaagctt tacccggatc
    24361 aacctgttga agtgctggct gccgacctgc gccgtgcgtt ctccggtatt gtggcgggta
    24421 acgtaaaaga agtcggtatt cgcgccattg aagagtttgg tccttacaaa atcaacggcg
    24481 ataaagagat tatgcgtcgt atggacgacc tgctacaggg ttttgttgcc cagcatcgta
    24541 tgaagttgcc aggctcagcc tacatccctt gctacgaaat ctgcacgtaa tctcccctcc
    24601 cccggtgacg tttgttgccg ggttttctcg tttttggaca cttactcatc aactcatttc
    24661 atttgttata tgaatgtttc ttaccaccct cacgcgacaa atatcatcac agttaatatg
    24721 tcatacaatt tatattgcaa cgcaaacgtt tccctatttt cataaaccgt atttta
//
LOCUS       DAZGBC010000068        24716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGBC010000068 DAZGBC010000000
VERSION     DAZGBC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.068"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..704
                     /gene="yiaT"
                     /locus_tag="ACHK1J_002610"
     CDS             <1..704
                     /gene="yiaT"
                     /locus_tag="ACHK1J_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO7911859.1"
                     /translation="PFMASATASELSIGAGAAYNESPYRGYNENTKAIPLISYEGDSF
                     YVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDKRDSTAMAGVAWYH
                     HERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYYDENFSDYYYG
                     ISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELPEEITDSPMID
                     RNESFTFVTGVSWRF"
     gene            complement(834..2804)
                     /locus_tag="ACHK1J_002611"
     CDS             complement(834..2804)
                     /locus_tag="ACHK1J_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO7911860.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNISRVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIVVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2815..4215)
                     /locus_tag="ACHK1J_002612"
     CDS             complement(2815..4215)
                     /locus_tag="ACHK1J_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7911861.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNQLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4441..5256
                     /locus_tag="ACHK1J_002613"
     CDS             4441..5256
                     /locus_tag="ACHK1J_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7911862.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5258..5398
                     /locus_tag="ACHK1J_002614"
                     /pseudo
     CDS             5258..5398
                     /locus_tag="ACHK1J_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            5500..5973
                     /gene="ysaA"
                     /locus_tag="ACHK1J_002615"
     CDS             5500..5973
                     /gene="ysaA"
                     /locus_tag="ACHK1J_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7911863.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(6124..7377)
                     /gene="avtA"
                     /locus_tag="ACHK1J_002616"
     CDS             complement(6124..7377)
                     /gene="avtA"
                     /locus_tag="ACHK1J_002616"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO7911864.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLHEKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7555..9585)
                     /gene="malS"
                     /locus_tag="ACHK1J_002617"
     CDS             complement(7555..9585)
                     /gene="malS"
                     /locus_tag="ACHK1J_002617"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7911865.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            9905..10729
                     /gene="bax"
                     /locus_tag="ACHK1J_002618"
     CDS             9905..10729
                     /gene="bax"
                     /locus_tag="ACHK1J_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO7911866.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(10837..12015)
                     /gene="xylR"
                     /locus_tag="ACHK1J_002619"
     CDS             complement(10837..12015)
                     /gene="xylR"
                     /locus_tag="ACHK1J_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO7911867.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(12093..13274)
                     /gene="xylH"
                     /locus_tag="ACHK1J_002620"
     CDS             complement(12093..13274)
                     /gene="xylH"
                     /locus_tag="ACHK1J_002620"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO7911868.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSTAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(13252..14793)
                     /gene="xylG"
                     /locus_tag="ACHK1J_002621"
     CDS             complement(13252..14793)
                     /gene="xylG"
                     /locus_tag="ACHK1J_002621"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO7911869.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(14871..15863)
                     /gene="xylF"
                     /locus_tag="ACHK1J_002622"
     CDS             complement(14871..15863)
                     /gene="xylF"
                     /locus_tag="ACHK1J_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7911870.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            16229..17551
                     /gene="xylA"
                     /locus_tag="ACHK1J_002623"
     CDS             16229..17551
                     /gene="xylA"
                     /locus_tag="ACHK1J_002623"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO7911871.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            17623..19077
                     /gene="xylB"
                     /locus_tag="ACHK1J_002624"
     CDS             17623..19077
                     /gene="xylB"
                     /locus_tag="ACHK1J_002624"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO7911872.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            19246..19587
                     /gene="yiaB"
                     /locus_tag="ACHK1J_002625"
     CDS             19246..19587
                     /gene="yiaB"
                     /locus_tag="ACHK1J_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO7911873.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFFGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            19633..20070
                     /gene="yiaA"
                     /locus_tag="ACHK1J_002626"
     CDS             19633..20070
                     /gene="yiaA"
                     /locus_tag="ACHK1J_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO7911874.1"
                     /translation="MDNKISTYSLAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(20112..21107)
                     /gene="wecH"
                     /locus_tag="ACHK1J_002627"
     CDS             complement(20112..21107)
                     /gene="wecH"
                     /locus_tag="ACHK1J_002627"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO7911875.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            21282..21581
                     /gene="ysaB"
                     /locus_tag="ACHK1J_002628"
     CDS             21282..21581
                     /gene="ysaB"
                     /locus_tag="ACHK1J_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO7911876.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            21676..22587
                     /gene="glyQ"
                     /locus_tag="ACHK1J_002629"
     CDS             21676..22587
                     /gene="glyQ"
                     /locus_tag="ACHK1J_002629"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO7911877.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            22597..24666
                     /gene="glyS"
                     /locus_tag="ACHK1J_002630"
     CDS             22597..24666
                     /gene="glyS"
                     /locus_tag="ACHK1J_002630"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO7911878.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         6103 a         6106 c         6381 g         6126 t
ORIGIN      
        1 tgccttttat ggcaagcgca actgcttctg aattatccat tggtgccggt gcggcttata
       61 atgaatcgcc ttatcgcggt tataatgaaa atacaaaggc aattcccctg attagttatg
      121 aaggtgattc tttttatgtt cgtcagacca cgttaggttt tattctgtcg caaagtgaaa
      181 aaaatgaact tagcctgacc gcatcctgga tgccgctgga atttgaccct gccgataatg
      241 acgattatgc catgcaacag cttgataagc gtgatagtac ggctatggcg ggggttgcct
      301 ggtatcacca cgagcgttgg ggaaccgtga aagcctctgc agctgcggac gttctggata
      361 acagcaacgg ctgggtgggg gagctatcgg tattccacaa aatgcagata ggtcgtctgt
      421 cgctgacacc tgcgctgggc gttctctatt atgacgagaa tttcagtgac tattactatg
      481 gcatttcaga gagtgagtcc cgtcgtagcg gtctggcaag ttattccgcg caggatgcct
      541 gggtgcccta tgtcagcctg acggcaaaat acccgatagg agagcacgtc gtattgatgg
      601 cgagcgcagg atacagcgag ctgccggaag agattaccga cagcccgatg attgatcgta
      661 atgagagttt tacctttgtc accggcgtga gctggcgttt ttaattcacc ggtggatgtc
      721 ggtgcggctg gcctacgctc tcgcaccgac ttgtatgcct gataagacgt gttaagcgtc
      781 gcatcaggca atgtgctcct tacgttggat gcggcgcgag tgccttatcc aacctacgga
      841 tggcaatgct tttcctcatt cacccagatc cgcatttcgc cttcaccccg gttggcccag
      901 ctaaaccacg ggataaaggt cagagtttgc gtctggcgtt tggctggcgc gctgtcgtaa
      961 tgccacagcg gttgctgctc tggattgctc tgttcatacc ggtaacccgg tgcctggatt
     1021 aagatcttat ggctaaacaa tcccttgcct tcaaatgtcg taaatggcgc atcggtgggc
     1081 agccacagat tatgcagtga ctcgccgttg tcggcctgtt ccaggcaata caccagcggg
     1141 ccgcgctgaa tcgccacttt tccggcgacg tgacgcacca gcgggttacc gtaaacgcgg
     1201 cgtaccggca tcggcaaagt cagattcagc gtatcgccct cctgccattc gcgggtaatg
     1261 tgcaaatacc ctttacgaat atcctgctcg acctcttccc cattcaatat gatctgcggc
     1321 tgtgtgcacc agtccggcag acgtaaagcc agcgtatgac gtaccggctg gggcgattcg
     1381 accacaatcg tcacctgctc ctgccacgga tagttcccgc taacccgcag gcgcagcgtg
     1441 ccattttcta ccggcacttc catgctgttt cctgcgtaga tgttgatata caacgcatct
     1501 tcacgcggcg tgtagagata atgaccaatc gaggtcagca cgcgggagat atttggcgga
     1561 caacaagcgc agccaaacca acgctggcgg atcggtttaa cgtgatcgta gatatggttg
     1621 aatttcagcg attttggatg tacttccagc ggattcacat agaagaaatg tttgccatcc
     1681 agtgccatgc caccgagcac agtgttgtac agcgcgcgct ccatcacatc ggcatattga
     1741 ctgtcgcctt ccatttccag cattcgtcgg gcgaacatca tcaggccgat ggaagcacaa
     1801 ctttcggcgt aaaccgtgtc attcggcaga tcgtaatcgc tgctgaacgc ttcgccgctg
     1861 ctctgtgagc caatgccgcc ggtaatatat aactgacgct gggccatatt gttccacagc
     1921 cgcaggcagt cctgacgctt gctgtcatcg tgacttaaac gcgcgagatg cgcgacgccg
     1981 gtcatcagat agacaaaacg taccgcgtga ccgatggcgg tttgctgttg cgcaaggggc
     2041 aaatgtgcct ggctgtaggc tttgtctttc accatccacg ccgggccgta ggtgtgccag
     2101 tgcgatgtct gcccgcgctt ttcatattcc tggtcgtaat agtgcggttg cgcaccacgc
     2161 tgttcgacaa aatagttcgt cagtgccagg tagcgcggct cttcagtcac ttcatacagg
     2221 cgcattagtg ccagttcaat ttccgggtga ccaggataac cgtgtaactt actttcatct
     2281 ggaccaaata cgctgtcgat atgatcggcc aggcggcaaa ccacttccag caagcgccgc
     2341 ttgcccgtgg cctggaagaa ggcgactccg gcttcaatca gatgacctgc gcagtaaagt
     2401 tcatgacact ccgccagatt gctccagcgt tcttcgggtg cttttaccgt aaagtaagta
     2461 ttgagatagc cgtcttcgca ttgggcggag gcgatcagtt cgattacctc gtcggcggtt
     2521 ttttccagtt cggcgtccgg cttctggcac agcgaccagg ctaccgcttc cagccatttg
     2581 gcgacgtcgc tgtcctgaaa caccatcccg taaaattcac cctcctgaag tccggcggca
     2641 atgcgaaagt tttcaatcgc atggctgggt tccgcttctg ggatacgatc gttcaaagca
     2701 tcccactgat aaggaatcac cacgtcgcgg accagttgtt ggtactgacc gaggaacgga
     2761 tcgctgaccg tcagtttatg cagatcgact tccgaaatgt tcatcttgtg ctccttaagg
     2821 ctgtacatga cgctgacgca ggtcggtagc tatgcgtgac atcataggat tattgagttt
     2881 gcaaaagcgg atcgcccctg ccagcagcag gtggaatagc gcaggcagta atgtttccat
     2941 cgccgcgatc ccgccaagcg aactggcggt ctggttttcc acgccaggct ggtaggcgac
     3001 aaaaataaac agcaggctga tgatcccggc gctggaggcc caggccagct tgatgaaaaa
     3061 cagattgaag gcgaagttca tgccggaaga acgtacgcgg gttttccact cgccgtagtc
     3121 atcggcaaag gccatcaatg agaagtgcag tggcaatgtg aagccaagaa ttacgccatt
     3181 accgaggatc accgccagcc acagcgtttg ataagccggg ccggagggca ggaaccacat
     3241 caacaccgcc agcgcagcga ggagctggtt ggtgtagtag taaattttga cggtatcgaa
     3301 acgccgcgtt aacgggctga caatcaccga gccgataatg gaggcgaagg tgaccatggt
     3361 gaagaacagc gacgtgtagc ccgtactgcc ttgtaagacg taggtaatga agtacatata
     3421 cccaccgccg cgaatgttaa agacgttaat cagcaggaaa gacatgacca gcatcagcag
     3481 cagttggtcg ttgttccgca gcccggcaag atgttcgcgc agggtaaatt tccccattgt
     3541 ggagagcggc acccgttcac gaacccagaa gaaacagcac aggaacatca ccacggcaat
     3601 ggcgcacaat accccgacgc ccagttgata gccgcgtgca gcgttgccct gaccgaagag
     3661 atctaccatc cacggtaagc ctaccgaaac cagaaatccc gctacgccgc agagaacgaa
     3721 tcgccaggac tggcaggaga tcacttcatt gtggcgggtg gtcatggtgt tgatcagcgc
     3781 gcagtacggt acgttgatgg cggtataacc gactgaaagg agcaggtaag taccgaacgc
     3841 ccaggcgatt tttactccca tgctggcatc tggcacggtg aacgtcagga tgccgataat
     3901 gccgatggga atggcaatcc atagctgcca cggacgaaag cgcccccagc gactttgcgt
     3961 ccggtcggca atgacgccca tcaccgggtc ggagatggca tcaaacacgc gcagtgcaat
     4021 aaacagcgtg ccaaccagcg ccggagttaa tccaaaaacg tcggtgtaga aaaaggtgag
     4081 aaaattcatg atcaggcaag ttattacagt gccgcctgcg tcacccaggc cgtagcctat
     4141 tttttcccgt acagataacg catcgtcaat tttaccttta gcgatatcag cggtagtaat
     4201 cggtgtagag gtcatagata ttcctcgtga atagggagca atttttagtt ttattgaaat
     4261 gacctgatta attctgccgt gaacgggaat atcaacaagg cgagaaaaga gtaaaaaaag
     4321 gtgacgattc cgacctgaat ttaaaagtgt gaaatggatc aggtgaaatt aatgtttttg
     4381 attaataatc aaaaggtaat gtgtttaatc attaatgaat aagcgggaaa ttacatatca
     4441 atgctcgaat tatccatgac gttaccgatt aaagtgcaaa atggtggatt atttatttcc
     4501 cgtggcgtag ggaggcatcc tgcaaggcgg ctggagtcat gggaaattat ctttgtcgaa
     4561 aaaggtcgat taacgattca ggaagaagaa cggatatttc tggtcgatgc gggagagagt
     4621 ttattactct ggccgaaccg acaacatgtt ggggtggagg attttcccgc cgatctcaaa
     4681 ttttactggc tgcattttga ggttaaagcg ccggacaacg accctcgctg gcttactcat
     4741 cttagcgtgc cgcaacaaac gaaggttgtc gatccgcagg cgctggtggc cctgtttcgc
     4801 cagttcttaa gcgaacagga aaagcatcaa cgcactccgg cgctggagtt tattgtgctg
     4861 ctcattttgc agcagctcac ccgtgacgcg cgtcttgatg aacaggccga tggcgcaggc
     4921 accacccttg cctggaaagc gcagaaactg atccgcacgc aatatcatcg cccgctttct
     4981 acggcgcttc tggcccgcga gctgtactgc aatgccgatt atcttgggcg cgtctaccgg
     5041 cgggtgtttc atctgacgtt aacagaagca atccatcgcc agcgggtgcg cgaagccgaa
     5101 aagctgttaa ttagcgaagc gcgatcgctc acggaagtgg cggcgttgtg cgggtttaat
     5161 gacgttggct atttccggca gatttttcgc aaacacaccg gtttaacgcc agcagcgtgg
     5221 aagcggcgtt acagcaagga acatatcaat tcgtagtgcc gtgtcgattt cgctttcgac
     5281 atcacgtctg aaacaggtgg gggagaaaaa tctcctgaaa ccactgcgtg aaaccgcgca
     5341 ggctatttct aatgaactgg gatttactgt cagggatgac ctgggcgcga tcacataacg
     5401 ctttggacaa gtgccaaaac tttaacattt ccttcgttgg atcaaagcag tagggacgtg
     5461 ctctctggca ctctgctgtt ttagtgcaaa ggagtgatca tgaaccggtt tattattgcg
     5521 gatgcgacga aatgtatcgg ttgccgtacc tgtgaagtgg cttgcgcagt gtcgcatcag
     5581 gagaatcagg attgcgctgc gttgtcacca gacgagttta tttcccgtat tcgtgttatt
     5641 aaagaccaca gctggaccac ggcagtagcc tgtcatcagt gtgaagatgc accgtgcgcg
     5701 aatgtctgcc ctgttgacgc gataagccgc gaacatgggc atattttcgt tgaacaatca
     5761 cgttgcattg gctgtaaaag ctgtatgctg gcttgcccgt ttggtgcgat ggaggtcgtt
     5821 tcttcgcgca aaaaggcgag ggcgattaag tgcgatctgt gctggcatcg ggagacggga
     5881 ccggcctgtg ttgaagcctg cccgacaaag gcgttgcagt gcatggatgt cgagaaagtg
     5941 cagcggcatc ggctacggca gcagcctgtt tgaaacgttt tccggcgttt ctaaacgccg
     6001 gaatagtgcg ctttgtttat gccagatgcg gcgtaaacgc cttatccggc ctacaaattc
     6061 ttccaaattc aatatattgc agaaatcatg taggcctgat aagcgtagcg catcaggcaa
     6121 tttttagtga ctttcagccc aggctctttc tatctcttcc gccagaatct tcacccccgc
     6181 ctcaattttc tccggctctg gtacgtagtt catgcgcata cattgatgcg tatgcggcca
     6241 cggtttatcc agccctggga agaagttgtg ccccggcacc atcagcacgc cgcgtgcttt
     6301 caggcgctga tagagctgct cggtcgtaat gggcaaatcc ttaaaccata gccagaggaa
     6361 aatggctcct tccggtttat gaatcaggca gcgattttcc ggtaaatagc ggcgaatgat
     6421 ggcgatagtt tcctgaacac gctggtagta aaacggtttg atgactgttt cagacaggcg
     6481 cagcagatcg ttacgcttaa tcatttcaca catcatcgcc ggaccaatac cgccaggtgc
     6541 caggctgata atgccgttca tattggtgat ggcggtgatg attttttcat tggcgatgat
     6601 aatgccgcag cgggagccag gtagacccag cttggaaaga ctcatgcaca gcacgatatt
     6661 cggattccat agcgggcgcg cttcactgaa gatgataccc gggaacggga caccataagc
     6721 gttatcaatc accagcggaa tgccgtgttg attcgccagc gcgtcaagct tcagcaactc
     6781 ttcgtcagta atcacattgc ctgttggatt cgtcggccgg gagacgcaaa tcatcccggt
     6841 ttcttcgcca atatgcagat gctcaaaatc gacgtggtat ttaaactggc cttcaggcag
     6901 cagttcaata ttcggacgcg cagagacaaa cagatcttct tccagtccgg cgtcagcgta
     6961 gccaatgtat tccggtgcca gcgggaacag cacttttttg acccgaccat cggcacggcg
     7021 tccggcaaac aggttaaata agtagaaaaa cgcgctctgg ctgccgtttg ttagtgcaat
     7081 attctgtggt tcgatatccc aacccaactt ctcgtgcagc attccggcaa gcagtgtgag
     7141 tagctccgtt ttcccctgtg gaccgtcgta attgcacagt gcatcagtcg ctttgccact
     7201 ttccagcatg tcggtcagta gcgtctggaa gtagtcctgc atttccggga tctgcgccgg
     7261 attaccgccg ccgagcataa tcgcgccagg cgtgcgtaaa ccgtcgttca gatcttccat
     7321 cagcagcgta atgccggagt ggcgggtaaa tttgtcacca aaaagggaga atgtcatagc
     7381 gggatatctg tcgaactctg tagcaaggaa ggtaacaata acgctacatt tgctggggtg
     7441 caaatcggtc tgttgaagag tgagtggtgc tttatcctgc aacgctgatt agggctgaca
     7501 ttttatcctg gttggcggct tcccgaggta ctgacgaacg ggaagccgga aaagtcactg
     7561 ttgcccagcc cagatgacca gcactttatc gtcgccatgc tcacgaacaa agccgtagcc
     7621 ctgcttcagc gaaagtgtcg tttgtttgcc cgcgccaatt gcgggatggc gggcgcggaa
     7681 ctggctgatt ttctgccagt gcgcgacgct ggcggcagat ttaccgctaa catcctgcca
     7741 gttcatatcc gaacgtgtac cttgcagcgg atcagaacct gtaggaccga acggacgcga
     7801 ggattcatca ccataaaaga tttgtaccgc gcctggcgct aataataata actctgctgc
     7861 tttgtcgccc ccttcacgga acaggcgggt atcatgcgac gagaggtagc tcaacacgtt
     7921 gaaaccctgc aatttctccg ccatttgctg ccaggtcgta tccatctgcg ccagacaatc
     7981 gactgctttc gccgcctgct cctgataatc gaaattgatc atcgcatcga agccgtggcg
     8041 atagtagtca ctttgcatca cgccgtggcc ccaggcttca ccggtcatcc agaaaggttt
     8101 gtcatctaat gctttgtcgg ggttagcttt tttccattcg cgaagcgcgg cgctggcttc
     8161 ggttttcagt tgctgccagg cgggcaactc aacatgtttg gcggtatcga ctcgaaaacc
     8221 atcaatccca tagtcgcgga cccactgact taaccagtgg gttaagtaat cgcgaggggt
     8281 aaagccgtcg atgactttag cgtgggtatc cgttttgttt ttatagaaca ccggcagacc
     8341 agaagcggtc gttgattcgg ttttgatatc cggcaaaaag gccagcgaca tagtgagatc
     8401 gtcaaatcca ggattgtcgt aatcgccgat atccgttctg atccagtttt ttccccacca
     8461 tttatcccag cctgttttgt cgctgaaatt aatgtaatcg ttaaagctat gccaggtttg
     8521 cccggcggca ggtttccagt cactccagcg ttcacccagc gtttttttca gctcgtcacc
     8581 agaaagatat aacgcgccaa actgatactc ctgcatatcc gccagcgtgg catagccggt
     8641 gtggttcatc acgacatcaa agagaatacg aataccgcgc tgatgcgcgc tatcaaccag
     8701 cgttcgtaga tcggcttcgt tgcccatatt ggcatcaaga ttcgtccagt cctgtgtgta
     8761 ataaccgtgg taggcataat gcgggaaatc accttttgta ccgccgccga cccagccgtg
     8821 aatttgctca aatggggcgc ttatccacaa agcattgacg cccagctgct ggagataatc
     8881 cagtttgttg gtcaggccgc gtaaatcgcc gccgtgaaaa gtacctattt ccgccatacc
     8941 gtctttatga cgtccgtaac tctggtcatt actgggatcg ccgttttcga aacgatctgt
     9001 cagcacaaag taaaccgtgg cgttatgcca gtcgaaaggg gcggatgtgt cagtttctgc
     9061 ccgttccagc aggagtaaac cgttgctggt ggcagcgggt tgtaacgtta tttgaccgtt
     9121 cttcactatc gcaatttgct ggctgtaata atcccgtacg gcggctcctt ccggaaaagt
     9181 ggcgctgaca tccagcgtga gcggtaatcc atcccatttc gggcattcac ggaccaggct
     9241 tgctaccggt tcggcggcgt tctggatgga aatcatcaat gttggcgtgc cggagcgggt
     9301 gtctatttgc agcgtatatt cgccgtccct gaacaatcgc cattgaggcg gcgtgttgct
     9361 acaaggttgc agggaaagca tctgattgag ttttatcgca tccgcaggct gccagcactg
     9421 ttggtcaaaa tttagcgtta gtggacgcgt acctttgggc aactgcgcgt ggctgacaaa
     9481 tgttcccgtt ccctgttcac taaaggcagg gaaccccgga gaagtccagc tggcggcaac
     9541 ggcgaagcca ggaaggagtg tcagaaaaca ggcggcgagt ttcatggcgg atgagtcctt
     9601 attgctgcga tttcagacag tttgccagcg ataagccaaa caaaactcat ccctgagcgg
     9661 taagtccaca ggatgagaag caagggtgag cgatctcgcg caaaaaacgg ctgaattttg
     9721 cgataaaccc cacattttct gcgatttagc gccaatctga atcgttaaca cgtgacatag
     9781 tttcagattt ggactattcc ttggggtgct cttgacagct atttttgata tgatttgaga
     9841 ttcaactctc aaatttgtga aaaatataag gtgttggaat gattaaatcc gaccaggaga
     9901 cctgatgatt ttgactccca tacgacgata tggggcgatg attcttatgt tactcactct
     9961 ggtgttttcg agtgaggtgt tagcgaagac gcacacaaca acagcgagtc aaaagtccca
    10021 cttaactaaa gctagtaata aacaggtaag cagtaaacaa gagtattctc gcaatagtgc
    10081 aaagagtagt tcacttcctg atttgcgaaa atacccttcc ggaacaccaa ggaaaaaggc
    10141 gtttcttcgg accgtaatgc cttacattac cagccaaaat gcagccatta ctgcagaacg
    10201 taactggctg atttcaaaac agtatcaggg ccaatggtca cctgctgagc gtgcgcgtct
    10261 gaaagacatc gccaaacgct acaaggtgaa gtggtctggt aatacgcgaa aaatcccgtg
    10321 gaataccttg cttgaacgcg tagacattat ccctaccagt atggtggcga cgatggctgc
    10381 agcagaaagc ggttggggaa cgtcgaagct ggcgcgcaac aacaacaacc tgttcggcat
    10441 gaaatgcatg aaaggacgtt gtaccaatgc gccaggtaaa gtgaaagggt actcacagtt
    10501 tagttctgtc aaagaatcgg tgagcgccta tgtcactaac ctcaatacgc atccggctta
    10561 ctcttcgttc cgtaaatcgc gtgcgcagct gcgtaaagct gatcaggaag tgactgccac
    10621 agcgatgatt cacaagctga agggctactc gaccaaaggg aagagttata acaactacct
    10681 gttcgcaatg taccaggata accaacggtt aatcgcggcg catatgtaat tgtatttcct
    10741 cgagccttat ccgacttgtc ggtcggataa ggcttttaat cgcatcaggt ggttgtgctg
    10801 agttacctga tgcgacgccg acaattctca tcatcgctac aacatgacct cgctatttac
    10861 atcgcgatac tcttttggcg tcgtgtcata tgctttttta aaaacagagt agaaatattg
    10921 cagcgatgga taaccgcaca tttgcgatat ctcattgatc gacaaggtgg ttgaaatcag
    10981 cagactgcgc gctttctcca gcttctcggc atgaatcatg gcatggatgg tttcacccac
    11041 ctcttcttta aaacgcttct caagattgga gcgcgagatc ccgaccgcat cgagtacctg
    11101 atccacttta atccctttac aggcgtgatt acgaatgtaa tgcatggcct gaataacagc
    11161 gggatcggtc agcgaacggt aatctgttga gcgccgttca atgacgcgaa ctggagggac
    11221 caaaatccgc tgtagcggca tttcttcttt atctaataat cgatgcaaca gttttgccgc
    11281 ctgatagccc atttgccgcg cgccctgagc gaccgaagaa agggcgacac gcgacagata
    11341 gcgggtcagt tcttcgttgt cgatgccaat cacgcataat ttttccggta cgggaatgtg
    11401 tagatgttca catacttgca gaatatgccg tgctcgggcg tcagtaacgg caataatccc
    11461 tgtttgcggt ggcagcgttt gtagccagtc tgccagccga ttttgcgcgt gttgccagtt
    11521 ctctggtgcg gtttctaacc cctgataaac cactccgcga tacttttctt cggcgacaag
    11581 ctgacgaaat gcatattcgc gttcagtggc ccaacgtttg ccgcttgatt ccggaagacc
    11641 ataaaaagca aagcggttaa cgcctttctc ttttaaatgc aaaaatgcgc tttcaaccag
    11701 cgcatagtta tcggtggcaa tgtaatgaac gggtgggtaa ctttctgcaa ggtgatacga
    11761 gccgccaacc ccaacaatgg ggacgtcgac atcagccagc gcttgctcga tctgtttgtc
    11821 gtcgaagtcg gcaatgacgc catctcctaa ccagtccttg attttatcaa tgcgggcgcg
    11881 gaaatcttct tcaatgaaaa tatcccattc cgattgtgac gcctgtaaat attcccctac
    11941 gccttctact acctgccggt cataggcttt attggcattg aacagtaatg tgatgcggtg
    12001 acgtttagta aacatggttc ttttcctgct gaatcatgca aaaactcaaa accggtaata
    12061 cgttaccggc tttgagaaaa tttttatcaa aatcaagaac ggcgtttggt tgcggagtcc
    12121 atccatactg ccagcaacag aatcgcacct ttaacgatat actgccagaa ggtcggtaca
    12181 tccatcatac tcataccgtt atccagtgaa gccatgatga atgcccccat taccgctcca
    12241 gccacgctcc cgacgccgcc tgccagactg gtgccgccaa tcacgcatgc tgcaattgcg
    12301 tccagctcgg cgatattccc cgcagaaggt gaaccagcgc caagtcgaga actgaggatt
    12361 aatccggcga tggctaccat taatccgtta atagcgaaca cggcaagttt ggtgcgttca
    12421 acgttaatcc cggagagacg tgctgcttcc agattgccgc cgatggcata aatgcgtcgt
    12481 ccaaatgctg tccgcgttgc cataaacatt ccgccgagta acagcaacgt cagcagcaga
    12541 acaggagtgg gaacgccacg ataatcattc aacagccaga ttgcgcctaa tacgatgata
    12601 gcggttaaag cctggcgacc gactactgcg gtagaggccg gagactgcaa acccaaagcc
    12661 tgacggcgca ttcttccgcg ccattgccaa ccaacaaaag ccattaagcc aagcgcgcca
    12721 atgatgaagc cggtgctggc ggggagatag ctttgcccaa tttgtgacat cgcggtgctg
    12781 gtgggggata cagtcgtgcc gttggtgatg ccaatgagta tgccgcgaaa tgccaacatg
    12841 cccgcgagag tgacaataaa tgaagggact ttgcggtacg cgacccacca tccgttccag
    12901 gcaccgagaa gcagtcccag aaccaacgtc acaatgatgg taagtggcaa aggccagcct
    12961 agccagacgt cacaaatcgc cgcgacgcca cctaatagcc ccatcattga gccgacggaa
    13021 aggtcgattt cagcagaaat tatgacgaac accattccta ccgcgaggat gccggtaatc
    13081 gcggtctggc gtaacaggtt ggagacgtta cgggcgctta agtaggcacc atcggtggtc
    13141 caggtaaaga acagcatgat tgcgatgata gctgcaatca tcacgaagac ctgcaaattc
    13201 agtgatttca gcccggagaa gccaccggat gtcggtacgg ccaatttcac ttcagacgga
    13261 ttgcttttcg acatgatgtt cgctcctcaa tgcggcttcc atcacctgct cctgagtcag
    13321 gttatgattt atcaggttag cttttagttt cccttcatgc atcaccagta cacgatcgct
    13381 aaggccgagc acttcaggta attcagaaga gatgacaata acggcaatac cctgctggac
    13441 gagttggtta attaatttgt agatctcgta tttcgcgcca atatcgatac ccctggtggg
    13501 ttcatcaaga atgagaatgc gcgggttaag taacagacag cgagcgagga tcgctttttg
    13561 ctgattgccg ccgctcaaac gtccaatagc aaggtcgggg gacgacgttt taactttgag
    13621 ttgctggatt gattccagaa tacatttttg ctctgccgcg tcatcaagct ggctaatgcc
    13681 accggtaaat ttattgagtg cggcgagggt aatattttta ccaaccgcca ttaccggaac
    13741 gatgccgtcg cgctttctgt cttcggggac catcgcaatc ccctgggcga tggcttgctg
    13801 acagttacga atatctacct gtttgccatc aatataaatt tttccttccc attgtccggg
    13861 ccacacacca aacaggcact gaatggtctc ggtacgtccg gcaccaacga gtccggcaat
    13921 acccagtatt tcgccacgtt tcagggaaaa cgagacatca ttaactcgtt taatatgacg
    13981 attaaccgga tgccatgccg tcagatgttc aatacgtaat atttcatctc cggtggtatg
    14041 tggttcatta gggtaaagcg cggttaactc tcgcccgacc atcatggtga taatatcgtc
    14101 ttcactcatt ccggcagcat cacgcgtacc aatgtgctgt ccgtcgcgaa taacgcaaat
    14161 cgtatcggaa atcgctttga cttcgttgag tttgtgcgaa atataaatac aggcgatacc
    14221 gtgctgttgt agatcgcgaa taatatccag taaaaccgac gtttcctgct cagttaatga
    14281 ggctgtcggt tcatcgagaa ttaacaagcg cacctgttta ttaagtgcct tggcaatttc
    14341 aaccagttgt tgttgcccaa gccctaaatc gccaacgcgg gtatcaggtg aaatggataa
    14401 actgacctgt gcgagcagct tctgacagcg tagcgtcatc aggtcataat ccataatgcc
    14461 attgtgggtt atttcgttac ccaggaagat attttccagc acggtcaatt ctttcaccag
    14521 ggccaattcc tggtgaatga tggcgatacc tttgcgttcg gtatcgcgga tgtgactcgc
    14581 ctgaatctct tctcccgcaa aaataatttc gccttcgtag gagccatggg gataaatacc
    14641 acacagcact ttcatcagcg ttgatttacc agacccattt tccccacaga gtgagacgat
    14701 ttcgccagca tttaaccgca agctgacgtt atcaatcgcc ttcacactgc cgaaggtttt
    14761 ggtaatgttc ttcatttcaa gtagataagg cataacgact ccacctaagc caattcattc
    14821 acgcggcatg gagagaaatc acgcccccgc tccgcgctgg ggcgtaacgc ttacagctcg
    14881 ctctctttgt ggaatccgtc tttaattacc gtatctttga tgttgttttt attcacatcg
    14941 atcggtgtca ggaggcggga ggggacatct ttcaggccat tattcagtga ggtatctgct
    15001 tttggctcct gaccattgcc caattcaacg gcaatttctg cggcagtatt tgccagcaat
    15061 gtaataggtt tatataccgt catagtttgc gtaccggcag caatacgttt aatacctgcg
    15121 agatccgcat cctggccgga aattgctact ttccctgata aaccttgcgc gcttaatgcc
    15181 tgaattgccc cacctgcggt ggcatcgttt gaggcaacta cagcatcaat tttgttatta
    15241 ttggcggtta gcgcgttttc cataattttc aatgcgtttt ccggtaacca gccatcaacc
    15301 cattggtcac caacgacttt aatttttccg gaatcaacgt aaggttttaa cactttcatt
    15361 tgtccggcgc ggaacagctt agcgttgtta tctaccggcg agccgcccat caggaagtaa
    15421 ttaccttgcg gaacaatatc gaccagggct tttgcctgca gttcaccgac tttttcgtta
    15481 tcgaaagaaa tataaaaatc gatatccgca tcgttaatca tacggtcgta agctaatact
    15541 ttaatgcctt cttgtttggc ttcttttaca acgttactta atacctgacc gttatacgga
    15601 ataatgacaa gaacatcgac accccggttt atcatgtttt caatctgcga catttgtgtt
    15661 tcttcattgc catttgcaga ctgtacaaat actttcgcgc cgagagattc tgcctttttc
    15721 acaaagatat ctcgatcttt ttgccagcgt tcaagacgga gatcatcaat cgccatacct
    15781 attttgactt ctttggcgtg tgcagcaacg ttggtaagca ggagtgaggt gcaaagggtg
    15841 agtagaatgt tctttatttt catggtgtag ggccttctgt agttagagga cagctttaat
    15901 aagtaacaat caccgcgata aacgtaacca atttttagca actaaacagg ggaaaacaat
    15961 tacagatttt tatctttcga ttacgatttt tggtttattt cttgatttat gaccgagatc
    16021 ttacttttgt tgcgcaattg tacttattgc atttttctct tcgaggaatt acccagtttc
    16081 atcattccat tttattttgc gagcgagcgc acacttgtga attatctcaa tagcagtgtg
    16141 aaataacata attgagcaac tgaaagggag tgcccaatat tacgacatca tccatcaccc
    16201 gcggcattac ctgattatgg agttcaatat gcaagcctat tttgaccagc tcgatcgcgt
    16261 tcgttatgaa ggctcaaaat cctcaaaccc gttagcattc cgtcactaca atcccgacga
    16321 actggtgttg ggcaagcgta tggaagagca cttgcgattt gccgcctgct actggcacac
    16381 cttctgctgg aacggggcgg atatgtttgg tgtgggggcg tttaatcgtc cgtggcagca
    16441 gcctggtgag gcactggcgt tggcgaagcg taaagcagat gtcgcatttg agtttttcca
    16501 caagttacat gtgccatttt attgcttcca cgatgtggat gtttcccctg agggcgcgtc
    16561 gttaaaagag tacatcaata attttgcgca aatggttgat gtcctggcag gcaagcaaga
    16621 agagagcggc gtgaagctgc tgtggggaac cgccaactgc tttacaaacc ctcgctacgg
    16681 cgcgggtgcg gcgacgaacc cagatccaga agtcttcagc tgggcggcaa cgcaagttgt
    16741 tacagcgatg gaagcaaccc ataaattggg cggtgaaaac tatgtcctgt ggggcggtcg
    16801 tgaaggttac gaaacgctgt taaataccga tttgcgtcag gagcgtgaac aactgggccg
    16861 ctttatgcaa atggtggttg agcataaaca taaaatcggc ttccagggca cgttgcttat
    16921 cgaaccgaaa ccgcaagaac cgactaaaca tcaatatgat tacgatgctg cgacagtcta
    16981 tggcttcctg aaacagtttg gtctggaaaa agagattaaa ctgaacattg aagctaacca
    17041 cgcgacgctg gcaggtcact ctttccatca tgaaattgcc accgccattg cgcttggcct
    17101 gtttggttct gtcgacgcga accgtggcga tgctcaactg ggctgggaca ccgatcagtt
    17161 cccgaacagt gtggaagaga atgcgctggt aatgtatgaa attctcaaag caggcggttt
    17221 caccaccggc ggtctgaact tcgatgccaa agtacgtcgt caaagtactg ataaatatga
    17281 tctgttttac ggtcatatcg gcgcgatgga tacgatggca ctggcgctga aaattgcagc
    17341 gcgcatgatt gaagatggcg agctggataa acgcatcgcg cagcgttatt ccggctggaa
    17401 tagcgaattg ggccagcaaa tcctgaaagg ccaaatgtca ctggcagatt tagccaaata
    17461 tgctcaggaa cataatttgt ctccggtgca tcagagtggt cgccaggagc aactggaaaa
    17521 tctggtaaat cattatctgt tcgacaaata acggctaact gtgcagtccg ttggcccggt
    17581 tatcggtagc gataccgggc atctttttaa ggaacgatcg atatgtatat cgggatagat
    17641 cttggcacct cgggcgtaaa agttattttg cttaacgagc agggtgaggt ggttgcttcg
    17701 caaacggaaa agctgaccgt ttcgcgcccg catccactct ggtcggaaca agacccggaa
    17761 cagtggtggc aggcaactga tcgcgcaatg aaagctctgg gcgatcagca ttctctgcag
    17821 gacgttaaag cattgggtat tgccggccag atgcatggag caaccttact ggatgctcaa
    17881 caacgggtat tgcgccctgc cattttgtgg aacgacgggc gctgtgcgca agagtgcact
    17941 ttgctggaag cgagagttcc gcaatcacga gtgattaccg gcaacctgat gatgcccgga
    18001 tttactgcgc ctaaattgct atgggttcag cggcatgagc cggagatatt ccgtcaaatc
    18061 gacaaagtat tattaccgaa agattacttg cgtctgcgta tgacgggaga gtttgccagc
    18121 gatatgtctg acgcagctgg caccatgtgg ctggatgtcg caaagcgtga ctggagtgac
    18181 gtcatgctgc aggcttgcga cttatctcgt gaccagatgc ccgcattata cgaaggcagc
    18241 gaaattactg gtgctttgtt acctgaagtt gcgaaagcgt ggggtatggc ggcggtgcca
    18301 gttatcgcag gcggtggcga caatgcagct ggtgcggttg gtgtgggaat ggttgatgct
    18361 aatcaggcaa tgttatcgct ggggacgtcg ggggtctatt ttgctgtcag cgaagggttc
    18421 ttaagcaagc cagaaagcgc cgtacacagc ttttgccatg cgctaccgca gcgttggcat
    18481 ttaatgtctg taatgctgag tgcagcgtcg tgtctggatt gggccgcaaa attaaccggc
    18541 ctgagcaatg tcccagcttt aatcgctgca gctcaacagg ctgatgaaag tgccgagcca
    18601 gtttggtttc tgccttatct ttccggcgag cgtacaccac acaataatcc ccaggcgaaa
    18661 ggggttttct ttggtttgac tcatcaacat ggccccaatg aactggcgcg agcagtgctg
    18721 gaaggcgtgg gttatgcgct ggcagatggc atggatgtcg tgcatgcctg cggcattaaa
    18781 ccgcaaagtg ttacgttgat tggtggcggg gcgcgtagtg agtactggcg tcagatgctg
    18841 gcggatatca gcggtcagca gctcgattac cgtacggggg gggatgtggg gccagcactg
    18901 ggcgcagcaa ggctggcgca gattgcggcg aatccagaga aatcgctcat tgagttgttg
    18961 ccgcaactac cgttagaaca gtcgcatcta ccagatgcgc agcgttatgc cgcttatcag
    19021 ccacgacgtg aaacattccg tcgcctctat cagcaacttc tgccattaat ggcgtaaacg
    19081 ttatcccctg cctgaccggg tgggggataa ttcacatcta tatatctcag caattaatta
    19141 atatttagta tgaatttatt ctgaaaatca tttgttaatg gcatttttca gttttgtctt
    19201 tcgttggtta ctcgtaatgt atcgctggta gatatggaga tcgttatgaa aacctcaaaa
    19261 actgtggcaa aactattatt tgttgtcggg gcgctggttt atctggttgg gctatggatc
    19321 tcatgcccat tgttgagtgg aaaaggctat ttttttggcg tgttaatgac agcaactttt
    19381 ggcaactatg catatcttcg cgcagaaaaa ctcgggcaac tggataattt ttttacccat
    19441 atttgccagt tagttgcgtt aatcactatc ggtctcttgt ttatcggtgt tttaaatgca
    19501 cctatcaatg cttatgaaat ggtgatctac cccatcgcct tttttgtctg cttgtttggt
    19561 caaatgcgtt tgtttcgctc ggtatgagca acataaagct cttacacatt caggaatgaa
    19621 aggaatactg tgatggacaa caaaatatca acctattcac tggcctttag tattgtgtca
    19681 tggatagctc tcgttggtgg tatcgttacc tatctgttag ggctatggaa tgcagagatg
    19741 cagttaaatg aaaaaggata ttattttgcc gtactggtat taggactgtt ttctgcggcg
    19801 tcttatcaaa agaccgttcg ggacaagtat gaaggcatac cgaccacttc catttattat
    19861 atgacctgcc tgactgtctt tattatctct gttgcattac tgatggtagg tctgtggaat
    19921 gcgacattat tactcagcga aaaaggtttt tatggactgg ctttcttctt aagcttgttt
    19981 ggtgcagtag cggtgcagaa aaatattcgt gatgccggaa taaacccacc aaaagaaaca
    20041 caggttaccc aggaagaata cagcgaataa ctcacgtaag cccggtcagt ccaatgtgac
    20101 cgggctttta cttaactcac taatctgttt ctgtcgattc gttgtaccag catagaaagt
    20161 aacaaactcg ctgccaacgt cgcgcaaaaa atccaaataa tatccagtat tggccaattt
    20221 ttaagctcaa ttccccgggt gcgcagtgca tggataatca aggcgtggaa tccgtatata
    20281 cccaacgaat ggcgggagat taagccaagt ccgcgaatgg tacgcgtatc cagcgtgttt
    20341 ttaaccagag tcaatagcgc gattgcgcag ataaaaacca tcggcccaca gtaaagatac
    20401 caggtatcgg caaaatttcc gcgccactgc aattcatata atgtcccgcg agagataata
    20461 aaaactcccg tcgcaaacag tgcggcgctc acccacgaca gtgctttatg ctgtgtgtcc
    20521 atcatcccta tagcgcggcc caacatgcca tacagaatgt agtaaaaagt atcgccattg
    20581 atatataagt taattggcag ccattcaaaa ccgtcgattt tctgcggcac ggtgtttggg
    20641 ttagcgatga taccaatcac caccattagc accagcaaca tttttccgcc gacgttcttc
    20701 acctgaatca gcggtgaaac cagataaatc accgcaatcg cgaagaaaaa ccataagtgg
    20761 taaaacactg gcttttgcag caggtttttc agcgctaact ccacattaat ggaggtaaac
    20821 agtgcaatgt agagcagcgc gattgcgcta taaaaaaaca gacataagcc gatacgcaag
    20881 aaatggcgcg gctgggcgct gcgttcgcca aaaaagagat agccggaaat catgaaaaat
    20941 agcggcacgc tgacacgaga ggcagagttc agaacattgg cgatatccca ggtgacgggg
    21001 ctaacactat gagcattggt cacataccag gtagtggtgt gaatcatcac caccattaaa
    21061 cacgctatcc ctcgcaggtt atcaatccag taaattttgg gctgcatctg tgtctctgta
    21121 tctggttaaa aaaagtctga ccgataaatc attcagttgg cgcactggaa taatctgagt
    21181 tttaacacta cagcttatag aggcttaagg aaattcgtaa gatatcagcc actataccga
    21241 tataaataat aagactcacc tgcaaaccag acggtaattt aatgatgatg aacgctttct
    21301 ttccggcaat ggcgcttatg gtgctagtgg gttgttctac accgtcaccc gtgcagaaag
    21361 cacaacgggt aaaggttgat cctctgcgtt cgttgaatat ggaagcgtta tgcaaggatc
    21421 aggcggcaaa acgttataac accggcgagc aaaaaatcga cgtcaccgcc ttcgaacagt
    21481 tccagggaag ctatgaaatg cgcggttata ccttccgtaa agagcagttt gtctgttctt
    21541 ttgacgcaga tggccatttt ttgcatcttt ccatgcgtta agtcctgctt tttcccgttt
    21601 catactgtat atcttccatc cagcgggtat actgatccct tcctttaaat ccacacgtat
    21661 ccagcacgaa ataatatgca aaagtttgat accaggacct tccagggctt gatcctgacc
    21721 ttacaggatt actgggctcg ccagggctgc accattgttc aaccattgga catggaagtc
    21781 ggcgcgggaa cctctcaccc aatgacctgt ctgcgcgcgc tggggccaga accgatggcg
    21841 gctgcttatg ttcagccttc tcgtcgcccg accgatggtc gctacggcga aaaccccaac
    21901 cgtttacagc actactatca gttccaggtg gtcattaagc catcgccgga caatattcag
    21961 gagctgtacc ttggttctct gaaagagctg ggcatggacc cgactatcca cgatatccgt
    22021 ttcgtggaag ataactggga aaacccgacg ctgggtgcct ggggactggg ctgggaagtg
    22081 tggctaaacg gcatggaagt gacgcagttc acttacttcc agcaggttgg tggtctggag
    22141 tgtaaaccgg ttaccggcga gatcacctac ggtctggaac gtctggccat gtacattcag
    22201 ggcgtagaca gcgtttacga cctggtctgg agcgacggcc cgctgggtaa aaccacctac
    22261 ggcgacgtgt tccatcagaa cgaagtggag cagtccactt acaacttcga atacgcggac
    22321 gtggacttcc tgttcacctg ctttgagcag tacgagaaag aagcgcagca gctgctggcg
    22381 ctggaaaatc cgctgccgct gccagcttac gagcgtattc tgaaagccgc ccacagcttc
    22441 aacctgctgg atgcgcgtaa agccatctcc gtcaccgagc gtcagcgcta cattctgcgc
    22501 attcgcaccc tgaccaaagc agtggcagaa gcatactacg cttcccgtga agccctcggc
    22561 ttcccgatgt gcaacaaaga taagtaagag gcggctatgt ctgagaaaac ttttctggtg
    22621 gaaatcggca ctgaagagct gccaccaaaa gcactgcgca gcctggctga gtcctttgct
    22681 gcgaacttta ctgcggagct ggataacgct ggcctcgcac acggcaccgt tcaatggttt
    22741 gctgctccgc gtcgtctggc gctgaaagta gctaacctgg cggaagcgca accggatcgt
    22801 gaaatcgaaa aacgcggccc ggcgattgcc caggcgttcg acgctgaagg caaaccgagc
    22861 aaagcggcag aaggttgggc gcgtggttgc ggtattaccg ttgaccaggc tgagcgtctg
    22921 actaccgata aaggtgaatg gctgctgtat cgcgcccacg tgaagggcga aagcactgaa
    22981 gcactgctgc cgaatatggt tgcgacttcg ctggcgaaac tgccgattcc gaaactgatg
    23041 cgctggggtg caagcgacgt gcacttcgtg cgtccggtgc acaccgtgac cctgctgctg
    23101 ggcgacaaag tcattccggc aaccattctg ggcattcagt ccgatcgcgt gattcgcggc
    23161 caccgcttta tgggcgagcc ggaattcacc atcgataacg ccgatcagta tccggaaatt
    23221 ctgcgtgagc gcgggaaagt catcgccgat tacgaagaac gtaaggcgaa gattaaagcc
    23281 gatgccgaag aagcagcgcg taagattggc ggtaacgctg acttaagcga aagcctgctg
    23341 gaagaagtgg cttcgctggt ggagtggccg gtcgttctaa ccgcgaaatt tgaagagaaa
    23401 ttcctcgcgg tgccgtctga agcactggtt tacaccatga aaggtgacca gaagtacttc
    23461 ccggtgtatg cgaacgacgg caaactgctg ccgaacttta tcttcgttgc caacatcgaa
    23521 tcgaaagatc cgcagcaaat tatctctggt aacgagaaag tcgttcgtcc acgtctggct
    23581 gatgccgagt tcttcttcaa caccgaccgt aaaaaacgtc tggaagataa cctgccgcgc
    23641 ctgcaaaccg tgctgttcca gcaacagctg ggtacgctgc gtgacaagac tgaccgcatc
    23701 caggcgctgg ctggctggat cgccgaacag attggcgctg acgttaacca cgcaacccgt
    23761 gcgggcctgc tgtctaagtg tgacctgatg accaacatgg tcttcgagtt caccgacacc
    23821 cagggcgtta tggggatgca ctacgcgcgt cacgatggcg aagcggaaga cgtagccgtc
    23881 gcgctgaatg agcagtatca gccgcgcttt gccggtgatg acctgccgtc caacccggta
    23941 gcttgtgcgc tggcgattgc tgataagatg gatactctgg cgggtatctt cggtatcggt
    24001 cagcatccga aaggcgacaa agacccgttt gcgctgcgtc gtgccgcact tggcgtgctg
    24061 cgaattatcg ttgagaagaa cctcaacctt gatctgcaaa cgctgaccga agaagcggtg
    24121 cgtctgtatg gcgataagct gactaatgcc aacgtagttg atgatgttat cgactttatg
    24181 ctcggtcgct tccgcgcctg gtatcaggac gaaggttata ccgttgacac catccaggcg
    24241 gtactggcgc gtcgtccgac tcgtccggct gatttcgatg cccgcatgaa agcggtatcg
    24301 cacttccgta ccctggaagc agctgcagca ctggcggcgg cgaacaaacg tgtatctaac
    24361 atcctggcga aatctgacga agtgctgagc gatcgcgtga atgcctctac tctgaaagag
    24421 ccggaagaaa ttaaactggc gatgcaggtt gtggtgctac gtgacaagct ggagccgtac
    24481 tttgcggaag gtcgttacca ggatgcgctg gtcgaactgg cagagctgcg tgaaccggtc
    24541 gatgccttct tcgataaagt gatggtcatg gttgatgaca aagaattgcg tatcaaccgt
    24601 ctgaccatgc tggagaaact gcgcgaactg ttcctgcgcg ttgcggatat ttcgctgttg
    24661 cagtaataac gccgttatta aaaagcctgc catctggcag gcttttttta tcgcta
//
LOCUS       DAZGBC010000069        24236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGBC010000069 DAZGBC010000000
VERSION     DAZGBC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.069"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..481
                     /gene="cmtB"
                     /locus_tag="ACHK1J_002631"
     CDS             38..481
                     /gene="cmtB"
                     /locus_tag="ACHK1J_002631"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO7911879.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSATDADSHIGAIQALSELLCEEEILEQLLTASSEKKLADIISRG"
     gene            509..1897
                     /gene="cmtA"
                     /locus_tag="ACHK1J_002632"
     CDS             509..1897
                     /gene="cmtA"
                     /locus_tag="ACHK1J_002632"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO7911880.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1912..3189
                     /gene="yggP"
                     /locus_tag="ACHK1J_002633"
     CDS             1912..3189
                     /gene="yggP"
                     /locus_tag="ACHK1J_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7911881.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3186..4151
                     /gene="glpX"
                     /locus_tag="ACHK1J_002634"
     CDS             3186..4151
                     /gene="glpX"
                     /locus_tag="ACHK1J_002634"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7911882.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVEVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4173..4682
                     /gene="fumE"
                     /locus_tag="ACHK1J_002635"
     CDS             4173..4682
                     /gene="fumE"
                     /locus_tag="ACHK1J_002635"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO7911883.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMNTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4679..5392
                     /locus_tag="ACHK1J_002636"
     CDS             4679..5392
                     /locus_tag="ACHK1J_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO7911884.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIKRVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5364..6041)
                     /locus_tag="ACHK1J_002637"
     CDS             complement(5364..6041)
                     /locus_tag="ACHK1J_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7911885.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     QPDDIHP"
     gene            complement(6035..6712)
                     /locus_tag="ACHK1J_002638"
     CDS             complement(6035..6712)
                     /locus_tag="ACHK1J_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7911886.1"
                     /translation="MVTLEQFRYCPTHTTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPHASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6700..7407)
                     /locus_tag="ACHK1J_002639"
     CDS             complement(6700..7407)
                     /locus_tag="ACHK1J_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO7911887.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLH"
     gene            complement(7408..7986)
                     /locus_tag="ACHK1J_002640"
     CDS             complement(7408..7986)
                     /locus_tag="ACHK1J_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO7911888.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPICTTRHFPAMAAVR"
     gene            complement(8009..8440)
                     /locus_tag="ACHK1J_002641"
     CDS             complement(8009..8440)
                     /locus_tag="ACHK1J_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO7911889.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8812..9831
                     /gene="epd"
                     /locus_tag="ACHK1J_002642"
     CDS             8812..9831
                     /gene="epd"
                     /locus_tag="ACHK1J_002642"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO7911890.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRSEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9881..11044
                     /gene="pgk"
                     /locus_tag="ACHK1J_002643"
     CDS             9881..11044
                     /gene="pgk"
                     /locus_tag="ACHK1J_002643"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO7911891.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11259..12338
                     /gene="fbaA"
                     /locus_tag="ACHK1J_002644"
     CDS             11259..12338
                     /gene="fbaA"
                     /locus_tag="ACHK1J_002644"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO7911892.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12402..12500)
                     /locus_tag="ACHK1J_002645"
                     /pseudo
     CDS             complement(12402..12500)
                     /locus_tag="ACHK1J_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12696..13556
                     /gene="mscS"
                     /locus_tag="ACHK1J_002646"
     CDS             12696..13556
                     /gene="mscS"
                     /locus_tag="ACHK1J_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO7911893.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13695..14330
                     /gene="argO"
                     /locus_tag="ACHK1J_002647"
     CDS             13695..14330
                     /gene="argO"
                     /locus_tag="ACHK1J_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO7911894.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14423..15163
                     /gene="yggE"
                     /locus_tag="ACHK1J_002648"
     CDS             14423..15163
                     /gene="yggE"
                     /locus_tag="ACHK1J_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO7911895.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15331..16227
                     /gene="ygfI"
                     /locus_tag="ACHK1J_002649"
     CDS             15331..16227
                     /gene="ygfI"
                     /locus_tag="ACHK1J_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7911896.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16224..17702)
                     /gene="scpC"
                     /locus_tag="ACHK1J_002650"
     CDS             complement(16224..17702)
                     /gene="scpC"
                     /locus_tag="ACHK1J_002650"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO7911897.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17726..18511)
                     /gene="scpB"
                     /locus_tag="ACHK1J_002651"
     CDS             complement(17726..18511)
                     /gene="scpB"
                     /locus_tag="ACHK1J_002651"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO7911898.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18522..19517)
                     /gene="meaB"
                     /locus_tag="ACHK1J_002652"
     CDS             complement(18522..19517)
                     /gene="meaB"
                     /locus_tag="ACHK1J_002652"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO7911899.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19510..21654)
                     /gene="scpA"
                     /locus_tag="ACHK1J_002653"
     CDS             complement(19510..21654)
                     /gene="scpA"
                     /locus_tag="ACHK1J_002653"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO7911900.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARVIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21858..22751)
                     /gene="argP"
                     /locus_tag="ACHK1J_002654"
     CDS             complement(21858..22751)
                     /gene="argP"
                     /locus_tag="ACHK1J_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO7911901.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            complement(22822..24147)
                     /locus_tag="ACHK1J_002655"
     CDS             complement(22822..24147)
                     /locus_tag="ACHK1J_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HFO7911902.1"
                     /translation="MKKANLTGTLVCFVACIIFLSSSDIFREKPIALIWFIRQNTLFF
                     TGTLAWCLMTLTMCLSLRSSWLNKVLGGLDKAWRLHKWAGICAIAFAFAHWLDEKLPQ
                     LFVAFGWLTHPGKIVDINLTPVQENWLHAGLLAGECAMFIMIAMVFVSLSKKVPYHLF
                     HLVHRLFPVFYLAIAFHVFTALFKSYWWETPAAYLLILITIPGVFVAFISLLKLNGNK
                     SKHQATVKSIVNHPGQITEVILEMEHPIDYCPGQFAFLTFAHSKESHPFTIASYVKEK
                     NTLRFAIKNLGDYTSTLASSIQIGQSALVEGPWGKFDFTLPCSRQVWIAGGIGITPFI
                     AQLEYLNQHGASRVPVDLWYCVSRSEDAWYVDKLTSLCAQASVTLHLLDAHKGERLQS
                     HYLTDKIANKGDTHFWFCGPQSFAKALSKGLYENGIASQNFHYDRFCMR"
BASE COUNT         5933 a         5958 c         6426 g         5919 t
ORIGIN      
        1 gccggacggt accctaaaaa taaaataagg ataatttatg cggcttagtg attattttcc
       61 agaatcatca atctcagtta tacattcagc aaaagattgg caggaagcta tcgatttctc
      121 gatggtatca ttgctggata aaaactatat cagcgagaat tacattcagg caattaaaga
      181 ttccaccatt aacaatggcc cttattatat tctcgcacca ggcgtggcaa tgcctcatgc
      241 gcgaccggaa tgtggggcgc ttaaaaccgg gatgtctttg acattacttg aacaaggtgt
      301 ttattttccg gggaatgatg aaccaattaa attactcatc ggactctctg ctaccgatgc
      361 cgattcgcac attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag aagaaatact
      421 cgaacaactc ttaacagcat catcagaaaa aaaattagcg gacattatca gccgcggata
      481 attactctct cctctacagg ataatattat ggaaaacaag tctgctcgtg caaaggtcca
      541 ggcttttggg ggctttttga ctgcaatggt catccccaat attggtgctt ttattgcctg
      601 gggttttatt actgcgttat ttattcccac cggttggctg cctaacgaac atttcgccaa
      661 aattgtcggc ccgatgatta cctatttatt gcccgtgatg attggttcta ccggtggtca
      721 tctggtcggc ggtaaacgcg gcgcggtcat gggcggaata ggtactattg gtgtgatcgt
      781 cggcgcagag atcccgatgt tccttggctc aatgattatg gggccgctcg gtggtctggt
      841 cataaaatat gttgataagg cactggaaaa acgcatacct gccggttttg agatggttat
      901 caataacttc tcattaggta tcgcggggat gctcctttgc ctgctgggtt ttgaagtgat
      961 cggcccggcg gtgttaattg ccaatacttt cgtcaaagag tgtattgagg cgctggtaca
     1021 tgcgggttat ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc ttttccttaa
     1081 taatgcgatc gatcagggcg tctattatcc gctgggaatg caacaggctt cggttaacgg
     1141 taaatccatc ttctttatgg tggcctctaa cccaggtccg ggcctggggc tgctgctggc
     1201 gtttaccttg tttggtaaag ggatgagtaa acgttctgcg cccggggcga tgattattca
     1261 cttcctcggt gggatccacg aactgtattt cccatatgtg ctgatgaagc cgctgactat
     1321 tattgccatg attgcgggcg gtatgtctgg cacctggatg tttaacttac tggacggtgg
     1381 tctggtggct ggcccaagtc cggggtctat ctttgcttac ctggcgctga cgccgaaagg
     1441 ttcgttcctg gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct ttgctatcac
     1501 ttcgctgata ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg agtttgctca
     1561 gtcagccaat gcggttaagg cgatgaaaca agagggtgca ttctcgttaa gcagggttaa
     1621 gcgtatcgcc tttgtttgcg atgcggggat gggctccagt gcgatgggcg cgaccacctt
     1681 ccgtaaacgc ctggaaaaag cggggctggc aattgaagta aaacattacg ccatagaaaa
     1741 cgtgcctgcg gatgcggata tcgtcgttac tcatgccagt ctggaagggc gcgtgaaacg
     1801 tgtgacggat aaaccactga tattgattaa taactatatt ggcgatccaa aactcgacac
     1861 tttatttaat caattaaccg ccgaacataa acactgatta cagaggtaaa aatgaaaacc
     1921 aaagttgctg ctatttatgg caagcgggat gtccgtctgc gcgaatttga actgccagaa
     1981 attaccgata atgaattact ggtgagtgtg atttctgaca gcgtctgttt atccacctgg
     2041 aaagcggcgt tactcggtag tgaacataaa cgcgtacccg acgatttaga aaatcatccg
     2101 gtcattaccg ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa tctcactgga
     2161 aaatataaaa aaggccagcg ttttgtattg caaccggcga tggggttacc aagcggatat
     2221 tcagcgggct acagctacga atattttggc ggcaatgcca cttatatgat tattcccgaa
     2281 atcgccatta atttgggctg cgtattaccg tatcacggct cttattttgc tgcggcgtcg
     2341 ctggcagagc ctatgtgctg cattattggt gcttatcatg ccaattatca caccacgcaa
     2401 tatgtttatg agcatcgcat gggcgtcaaa cctggcggca atattgcact gctggcgtgt
     2461 gcaggtccga tgggcattgg cgctatcgat tacgccatta acggcggcat acaaccgtcg
     2521 cgggtggtgg tggtcgatat cgacgacaaa cgtctggcgc aggtacagaa gctgctgccg
     2581 gtggaactgg cggccagtaa aggcattgag ctggtgtatg tgaataccaa agggatgagc
     2641 gatcctgtcc agatgctgcg ggcgctgaca ggagatgccg ggttcgatga catttttgtt
     2701 tatgcggcgg tgcctgctgt cgttgagatg gctgatgaat tactggcgga agatggctgt
     2761 ctgaacttct ttgccgggcc gacggataaa aacttcaaag tgccgtttaa tttctacaac
     2821 gttcattaca acagcacgca cgtcgtcggt acatccggcg gttcaacgga cgacatgaaa
     2881 gaggcgattg cccttagcgc cactgggcag ttacagccgt cgtttatggt gacccatatc
     2941 ggtggcctgg atgcggtgcc agaaaccgtg ctcaatctgc cggatatccc tggcggtaaa
     3001 aaactcattt ataacggcgt gaccatgccg ctcactgcca ttgccgattt tgccgaaaaa
     3061 ggcaaaaccg atccgctgtt taaagagttg gcgcggctgg ttgaggaaac gcacggcatc
     3121 tggaatgaac aggccgagaa atatctgctg gcacaatttg gcgttgatat tggggaggcc
     3181 gcgcaatgat gtccctggcg tggccattat ttcgcgttac ggaacaggca gcgttggctg
     3241 cctggccgca aaccggatgt ggcgacaaaa ataaaattga tggcctggcg gtcaccgcga
     3301 tgcgccaggc attaaacgac gtcgcttttc gtggacgagt ggttatcggc gaaggagaaa
     3361 ttgaccatgc gccgatgctg tggattggcg aagaggtggg caaaggcgat gggccagaag
     3421 tcgatatcgc ggttgatccc attgaaggca cgcggatggt ggcgatgggg cagagcaatg
     3481 cgctggcggt catggctttc gcccctcgag atagcctgtt acatgcgccc gatatgtata
     3541 tgaaaaagct ggttgttaat cgactggctg ccggggcgat tgatctgtct ttgcctctgg
     3601 cggacaactt gcgtaacgtg gcgcgggcgt taggtaaacc gctggacaag ctgcgcatgg
     3661 ttacgctgga taaaccgcgt ctgagtgccg cgattgagga agcaacacag ctgggggtga
     3721 aagtttttgc cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc tggcaggata
     3781 atccttacga tgtgatgtac accatcggcg gtgcgccgga aggcgtgatt tctgcctgtg
     3841 cagtcaaagc gttaggcgga gatatgcagg cggaattaat cgatttttgt caggctaaag
     3901 gggattacac ggaaaatcgg cagattgcag agcaggagcg caagcgttgc aaggcaatgg
     3961 gcgttgaagt gaaccgtgtg tactcgctcg acgaactggt gagggggaac gatatcctct
     4021 ttagcgctac gggcgtcacg ggcggcgagc tggtgaacgg tatccaacag acggcgaatg
     4081 gggtgcggac gcagacatta ctgatcggcg gcgcggacca aacgtgtaat ataatagact
     4141 ccctgcattg atggtgatct atcggaagac caatggcgac gctgacagaa gatgatgtgc
     4201 ttgagcaact ggatgcacag gacaatttat tttcatttat gaatactgcg cattctattt
     4261 tgctccaggg gatacgccag tttctgccgt cgctgtttgt cgataacgat gaagagatcg
     4321 tcgaatatgc agtgaagccg ttactcgccc aaagcggccc gcttgacgat attgatgttg
     4381 cactgcgttt gatttatgcg ctggggaaaa tggataaatg gctgtacgcc gatatcacgc
     4441 atttttccca gtactggcat tacctgaaag aacaggatga aacgctcgga tttgccgatg
     4501 acatgacttg ggattttatc agcaacgtca atagcataac ctgcaatgcg acgctctatg
     4561 acgcgttgaa agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg cgttttagcg
     4621 ggatggtcaa aacggcgctg acgctggcag taacgaccac cttaaaggaa ttaacgccgt
     4681 gaaaattgaa ttaacggtga atgggctgaa cgtgcaagcg cagtaccatg atgaagagat
     4741 aaaacgcgtg cataaaccgt tgctgcgtat gctggcagcc ttacagacgg taaatccgca
     4801 gcggcgtact gtggtttttc tttgcgctcc gccggggacg gggaaatcta ccctaaccac
     4861 cttctgggaa tatctcgcgc aacaagaccc ggaactgccc gctattcaga cgctgccgat
     4921 ggatggtttt caccattaca atagctggct ggatgcgcat caattgcgcc ccttcaaagg
     4981 cgcaccagag acatttgacg tagcgaaact ggcggaaaat ctgcgccagg tcgtggaagg
     5041 ggattgtacg tggccgcagt atgatcgaca aaagcatgat cctgttgaag atgcgttgca
     5101 cgttaccgca ccactcgtca tcgtcgaagg aaactggttg ttgctggacg acgagaagtg
     5161 gtgccagctt gcgcaatttt gtgatttctc gatctttatc aatgcgccag ctactgcgtt
     5221 gcgggaacga ctggtggggc gcaagctggc gggagggtta tcgctggcag acgcagaagc
     5281 tttttatgac cgtaccgacg ggccgaatgt tcgacgggtg ctggaagaga gtctgcctgc
     5341 aaatttgacc ttaatgatga ccgctacggg tgaatatcgt ctggttgatt aacgttcctg
     5401 attagaccat cttccagtcg taccacacga gagaaatgac gccgggtaaa tgcggcgtca
     5461 tgactgattg ctacgacgct cgtgcctcgc tggcggcact tctccagcca gctttcaaac
     5521 acgctcaacc aattttcgtc aaaatcccga ctcggctcgt ccagcagcaa tagtggcgga
     5581 gagagtgctt ccaggcaggc gacggcgacc atccgtcgct gcgcactatg cagatccaat
     5641 gggtgcgcgc tggcagtgtc tgctaactga caacattgca gggcagcatg ggtacgttga
     5701 gtgatttcat ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc gtcagcgacg
     5761 gtgctgtgaa aaagttggtt ttccgcttcc tgaaacagta cgccaatctt tgccgcgcgc
     5821 tggcggtttt tcaggttttt catcgcctgt tgttgcagca taaccgtacc ggctgtgggc
     5881 gtaagaagac ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt gtcaccggtc
     5941 agcgccaacc attcaccttg tttgagttgc agagaaatgt cgcaaagaca atccgttgcc
     6001 gcgccaggcc agcgataaga tatctgattt aacgttaaca tagtggctgg agcgctccgt
     6061 tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga cgttcaaaaa
     6121 gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac agcatttcgc
     6181 tggcatgcgg cgtcaggcgg ctaaaggctt catcaagaat taatagcttt ggctgcatcg
     6241 cgatggcgca ggcgatgacc acgcgctgtg tctctccacc ggagagtgtt gctgggtgac
     6301 gatggcgcaa cggctggcac tcggtcagag tcagggcggc atcaatccgc gccataatct
     6361 ctgcttcagc aagacacaga ttttctggcc caaaggcaac ttcttcttcc acgctaaagg
     6421 tacagccgga gagctgcaaa tagggcgatt gctggacgag ctggatggtg gcggattgtt
     6481 cattaagcgg gagatgccca atgggcgtac cgaggagcgt ccccgtgcct gttatctctc
     6541 ctggcagaaa gtccggatac cagcccgcca tcagctgtgc cagcgtactt ttgccactgc
     6601 cgttgtcacc aaagatggcg accatccccg gtttgacata gtgaaagtca taacagaacg
     6661 gaggatgcgt tgtgtgtgtc gggcaataac ggaactgttc taatgtaacc atatccacgc
     6721 tccgaattct gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt gtagggtgct
     6781 atcggctggt gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc gcatatccag
     6841 cgccgcacca cggacagcaa gatcgtttag cgcattgtgt gttagcggaa taattaacgc
     6901 gggcatcgca cgtaaacgct gataccagcc ctcatccagc ggcacgccgc gggcacgctg
     6961 cgcttcatga ataatcgcta attgccgttt taactgctca acgaccaata acggcccggc
     7021 aaacagataa gcgacgcctg gcggcaggcg agaagcaaac agagcgcgga taaaacattg
     7081 cacggggacg tactgcatcc acaactgtga ggttgaaaca atcgccagaa tccgcaacca
     7141 gagcgtaata gcatgcgtcc aacgctccgg cgaacgcggc gtgccgctta gccattctgt
     7201 tagccagcca ccgtgtacca gccacagccc ggctcccagc gagaacatca gccagacgac
     7261 atattttgcc cgccgccgcg tggcctttag cgcaataagg caaaaaaagg tcgccgcgct
     7321 atagatcggc aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg ccagcgccca
     7381 cagcgttaag gatgtaaacg gatgcattta gcgcacggca gccattgccg gaaaatggcg
     7441 cgtggtgcaa atcggcagct ggcgcagtaa aagccagaca atcaccgctg taagaatttt
     7501 atcgaccagg tttgcgccta tcaccgtaat cgccaccgac tccaccaggt tttgccccat
     7561 agagtgcatc caggcaacga acaaatcggc tccgctaccg gtgacaccgc cgaacagcgc
     7621 agtacgcagc ggtacagcga cgaccgttac cgcaagcgta ataatcacgc cgctgacaac
     7681 gacttttggc agggtgcgga accaacccgc tcgcgccagc caaccggcga ccaggccgat
     7741 gaccatcgcc acgggtgcaa atgcggcggc gatcgggtcg gtcagcaatc cccagagtaa
     7801 attggtcagt aagccggtca gcattccgat caccggacca agcagtaccg cgctaataag
     7861 tgtgccgatg gagtcgagaa atataggtaa tttgaccatg ctggccagtt gcccgccgat
     7921 catattgatg gcgatagata taacgatcaa caccagtgcc tgactggaaa agtggctacg
     7981 cgccataaga aaacctctct gaaagtagtt atttaacggg ggaaatatgc aactcgtcgt
     8041 aaccagaaag cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta ccgatcacca
     8101 gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg accgccaagc atcgtgccgt
     8161 gtggatcgga aacgcagaga tgaagatgct caccactctg ttccaacgtg ccgtttagtg
     8221 agatcacttc gaatgtaccg ttcaggagcg tggtgccttc ttgcccggca tagcgcaagg
     8281 cgatgtcagt caggctaccg gtacagcctg caatccaggc ggcatgaagt tgctgctgct
     8341 gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc agtgcgtaaa
     8401 agcgtgcgga ggaagaatat gggtagggca atgtcatcat tggagtactc cgaaactcgg
     8461 acgtattgcg gataatatcg acgctgcatc agtcatattc tgatataagt cagggtttgt
     8521 gacggcacaa cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt ggaataaatt
     8581 ccacaggggc ggggctgaaa acgtgacgta agtcagataa ttgttttctt tgactggaca
     8641 atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt cgattaaatg
     8701 ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg gacgatttgc
     8761 tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc tatgaccgta
     8821 cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc tttgtatgaa
     8881 tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga tgctgcgggc
     8941 atggcgcatt tgttgaaata cgacaccagc catggccgtt ttgcatggga agtacgacag
     9001 gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga acgttcactg
     9061 caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac cggcgtatat
     9121 ggctcccgcg agcatggcga agcgcacatt gccgccgggg ctaaaaaagt gctcttttca
     9181 catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca ggatcaactt
     9241 cgttcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat aattcccgtc
     9301 atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac aattcactcc
     9361 gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg cacccgggca
     9421 gccagccagt cgatcattcc ggtcgatact aaactggccg ccggtatcac acgatttttt
     9481 ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat aaatgtgacg
     9541 gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt caacctgttg
     9601 ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga attgccgttg
     9661 gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac ccaaacccgg
     9721 gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg gggctttgct
     9781 aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta agacgcaagc
     9841 agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa ttaagatgac
     9901 cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg taccagtaaa
     9961 agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca tcgaactggc
    10021 cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta ccgaaggcga
    10081 gtacaacgaa gaattctctc ttctgccggt tgttaactac ctgaaagaca aactgtctaa
    10141 cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag gtgaactggt
    10201 tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa ccctgtccaa
    10261 aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg ctcaccgcgc
    10321 gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag gcccgctgct
    10381 ggcagctgaa ctcgacgcgc tgggtaaagc actgaaagaa cctgctcgcc cgatggtggc
    10441 tatcgttggt ggttctaaag tatctaccaa actgaccgtt ctggactccc tgtctaaaat
    10501 cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg cacaaggcca
    10561 cgatgtgggt aaatccctgt acgaagctga cctggttgac gaagcaaaac gtctgctgac
    10621 cacctgcaac atcccggttc cgtctgatgt tcgcgtagca accgagttct ctgaaaccgc
    10681 tccggctacc ctgaaatctg ttaacgacgt gaaagctgac gagcagatcc tggatatcgg
    10741 tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca ttctgtggaa
    10801 cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa tcgtggctaa
    10861 cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc tggcagcaat
    10921 cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg gcgcattcct
    10981 cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc gcgctaagaa
    11041 gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag cgcgctttta gagcacgcat
    11101 tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga atccccagga
    11161 gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc aacttgaagt
    11221 atgacgagta taaggcccga cgatacagga caagagacat gtctaagatt tttgatttcg
    11281 taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta gcaaaagaaa
    11341 acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac gccgtactgg
    11401 aaaccgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt ggtgcttcct
    11461 ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc ctgggcgcga
    11521 tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg gttatcctgc
    11581 acactgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg gacgcgggtg
    11641 aaaaacactt cgctgctacc ggcaagccgc tgttctcttc tcacatgatc gacctgtctg
    11701 aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgc atgtccaaaa
    11761 tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac ggcgtggaca
    11821 acagtcacat ggacgcttct gcactgtaca cccagccgga agacgttgat tacgcataca
    11881 ccgagctgag caaaatcagc ccgcgtttca ccatcgcagc gtccttcggt aacgtacacg
    11941 gtgtttacaa gccgggtaac gtagttctga ctccgaccat cctgcgtgat tctcaggaat
    12001 atgtttctaa gaaacacaac ctgccgcaca acagcctgaa cttcgtattc cacggtggtt
    12061 ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg aaaatgaaca
    12121 tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac aaagcgaacg
    12181 aagcttatct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg aacaagaaat
    12241 actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct cgtctggaaa
    12301 aagcattcca ggaactgaac gcgatcgacg ttctgtaagt cattcctttc tgcttatctc
    12361 aaggcccgct ctgcgggtct ttttttcgcc aaaagaaaaa ttgcctgatg cgctacgctt
    12421 atcaggccta cacgtatcct gcaatatatt gaattcgcag gtttttgtag gccggataag
    12481 gcgttcacgc cgcatccggc atgacaaagc gcacattgat atctgtctga gccccgctct
    12541 gcggggattt tttcgccaaa agcaaggtga tacagatgag aaatctgtga tctatttggc
    12601 aaaattatgc tttattgttt acccttgtca gactgcccgt cataaggcgg cggagtgtat
    12661 ttctccattt tgagtcagtt gaaaaggaat attgaatgga agatttgaat gttgtcgata
    12721 gcataaacgg cgcgggaagc tggctggtgg ctaaccaggc gctgctgcta agttatgcag
    12781 taaacatcgt ggcggcactc gcgatcatca tcgttggttt gattatcgcg cggatgattt
    12841 ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga tgccactgtt gctgattttc
    12901 tttctgcatt agtccgttac ggtattatcg cctttacgct aatcgctgca ctgggacgcg
    12961 tgggtgtaca aaccgcgtcc gtcattgctg tactcggtgc cgcaggctta gctgttggtc
    13021 tggctttgca ggggtcactg tctaacctgg ccgctggcgt gttacttgtc atgttccgcc
    13081 cgttccgtgc cggagaatat gttgacctcg gcggcgtagc cggtactgtg ctgagtgtgc
    13141 agattttctc caccaccatg cgtactgcag acggtaaaat tatcgttatt ccgaacggta
    13201 aaattattgc cggaaatatt attaacttct cccgcgagcc agttcgccgt aacgagttta
    13261 ttattggcgt ggcgtatgat tccgatatcg atcaggttaa gcagatcctg accaatatta
    13321 tccagtctga agatcgcatt ttgaaagatc gcgaaatgac tgtgcgcctg aacgaacttg
    13381 gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa cagtggcgat ctgcaaaacg
    13441 tgtactggga tgtgctggag cgtattaaac gtgaatttga tgccgccggt atcagcttcc
    13501 cgtacccgca aatggatgtg aactttaagc gggtgaaaga agacaaagct gcgtaatcaa
    13561 cgctgaggcc agataatact ctctggcctc tctcttatta gtttttctga ttgccaatta
    13621 atattatcaa tttccgctaa taacaatccc gcgatatagt ctctgcatca gatacttaat
    13681 tcggaatacc aaacgtgttt tcttattact ttcaaggtct tgcgcttggc gcagctatga
    13741 tcctaccgct cggtccacaa aatgcttttg tgatgaatca gggcattcgt cgtcagtacc
    13801 acattatgat tgccttactt tgcgctatca gcgatttggt cctgatttgc gccgggattt
    13861 ttggtggcag cgcgttattg atgcagtcgc cgtggttgct ggcgctggtc acctggggcg
    13921 gcgtagcctt cttgctctgg tatggttttg gcgcttttaa aacagcgatg agcagtaata
    13981 ttgagttagc cagtgccgaa gtcctgaagc aaggaagatg gaaaattatc gccaccatgt
    14041 tggcagtgac ctggctgaat ccgcatgttt acctggatac ttttgttgta ctgggcagcc
    14101 ttggcggtca gcttgacgtc gaaccaaaac gttggtttgc gctcgggaca attagcgcct
    14161 ctttcctgtg gttctttggt ctggctattc tcgcagcctg gctggcaccg cgcctgcgca
    14221 cggcaaaatc acagcgcatt atcaatctgg tagtgggatg tgtcatgtgg tttatagcct
    14281 tgcagctggc gagagacggt attgctcatg cacaagcctt gttcagttag gcgctatctg
    14341 atggaaaaat aaaacagagg cgctaagctt gcctccagag gtcctgaatt ttaggcaagt
    14401 caaataacac ggagagacta acgtgaagtt caaagttatc gccctggcgg cattaatggg
    14461 tattagcggg atggcagcgc aggctaacga attgccggat ggaccgcata ttgtcacctc
    14521 cggtacggca agcgtggatg cggtgccaga cattgccact cttgcgattg aagtcaatgt
    14581 ggccgcaaag gatgccgcta ctgccaagaa acaggcggat gagcgcgtcg cacaatacat
    14641 ttccttcctt gaactcaatc agatcgcgaa aaaagatatc agctcagcga acttacgcac
    14701 ccagccagat tatgattatc aggatggtaa aagtatcctt aaaggctacc gcgctgtgag
    14761 aacggtggaa gtcacgctcc gtcagttaga caaactgaat tccttgctgg atggcgcgct
    14821 gaaggcgggt cttaacgaaa ttcgttctgt gtcgctgggc gtggcgcagc cggatgccta
    14881 taaagacaaa gcacgtaagg cagcgattga taacgcgatt catcaggcgc aggaactggc
    14941 gaacggcttt catcgtaaac tggggccggt atatagcgtg cgctaccatg tttccaacta
    15001 tcagcccagc ccaatggtgc ggatgatgaa agccgatgcc gcgccggtct cagcccagga
    15061 aacttacgag caggccgcta ttcagtttga tgatcaggtc gatgtggtct tccagttaga
    15121 acctgtggat caacaacccg ctaaaacacc tgcagcacaa taatacttgt agcccgacgt
    15181 attcgtcggg ctaatgcctt tacccgatat tgcctccttt ttctgtaatt ttctgtaaaa
    15241 ccatgacgta atcacctatc tttgctcgcg gtttcatcaa ggataggctg actgaaacgg
    15301 ttaagcggac aaggatgcgg gggagggggg atggacattt ttatttcgaa aaagatgcgc
    15361 aatttcattt tattagcgca aaccaataat attgcccggg cggcagaaaa aatccatatg
    15421 acggcttcgc catttggtaa aagcattgcc gcgctggaag aacaaattgg ctatacgcta
    15481 tttacccgca aagataataa tatcagcctc aataaagccg gacaggaact gtatcaaaaa
    15541 ctgttcccgg tttatcaacg gctttctgcg attgataatg aaatccataa ctcagggcgt
    15601 cgttcacgag aaattgtgat tggcatagat aatacttatc cgacaattat tttcgatcag
    15661 ttgattagtc tcggagataa gtatgaaggc gtgaccgccc agccagttga gttcagcgaa
    15721 aacggcgtta tcgataatct ttttgaccgc cagttagatt ttattatctc cccacagcat
    15781 gtatctgcac gagttcagga actggaaaac ctgactatca gtgaactacc accgctgcga
    15841 ttgggctttc tcgtttctcg ccgttatgaa gagcgtcaag agcaagagct gcttcaggag
    15901 ctgccctggc tacaaatgcg ctttcaaaac cgggctaact tcgaagcaat gattgatgcg
    15961 aatatgcgac cttgtgggat caatccaacc attatttatc gtccctacag ttttatggcc
    16021 aaaatcagcg ccgtggagcg cggacatttt ctgacggtga tcccgcattt tgcctggcga
    16081 ctggtgaatc cggcaacgct gaaatatttt gatgcgccgc acaggccgat gtatatgcag
    16141 gaatatcttt attcaatcag aaatcatcgt tataccgcca cgatgcttca gcatattgct
    16201 gaagatcgtg acgggacaaa ccattaaccc agcatcgagc cggttgcaat taaattacgg
    16261 tgtaagtcga agacgtggct aagatcgtgg tgaatatgtc cgccaggcgc attttccaga
    16321 tagcgatgca gataatcccg atacatagga tgtgcacaat tatcaatgat cgtgcgggcg
    16381 cgttgaagcg gggaaagacc gcgcagatcg gcgatccctt gttcggtgat gatcactttg
    16441 acgctgtgtt cgctgtgatc aacatggctg cacattggca cgacggttga gatcttgcct
    16501 tctttagcaa tcgatggggc cataaagatc gacagatacg cgttgcgttc aaaatcaccg
    16561 ctgccgccga tgccgttcat cagatcgacc ccggctacgt gcgttgagtt ggcatgcccg
    16621 taaatatcaa actccaggcc gacgttcaga gcgatgacgc ccagacgacg gatgatttcc
    16681 gggttattgg aaatctcctg cggacgcaac acaatgcggc tggcaaagta atccatattg
    16741 tcgtaaatct tgcgcaggga atcggccgag attgtcaggc tggaggcgct ggccccgctg
    16801 attttgccgg tttccagtaa atgcaccacc gattcctgta gcacttccga atacatcata
    16861 aacggaggaa tttccgggtt ttcccccaga cgcgccatta ccgcattatt gatattgccc
    16921 acgccacttt gcagcggcag aaattccggc ggaatacgcc catgcgccat ttcctgcaat
    16981 aagaacgtga ccacgttatc ggcaatctgc tggcacatgg gattttgctt atccagcata
    17041 ttaccggcgt cgggcaagtt ggtttccacg acggcgacaa tctttttcgg atcgatttgc
    17101 acatagcggg taccgacgcg atccattgca tggaagatcg acacgctatt acgccgcggt
    17161 ggcgcgccag gaatcacaat atctgccagt tctgcaacgc gcggatcgtg atagtgatta
    17221 agttcaatga tcactttctt cgcccgcagc agccatgtcg gcgcattacc gatcccgctg
    17281 gttaaccaga ctcgaccatc cggtgccagt gccgatgctt caatgacggc aacatcaatg
    17341 tcgccgaaga aaccgtaatt gaccatttgc gccacttcgc tcaaatgcag gtcaacgaaa
    17401 ctcaccgcgc cctgattgat ctttttacgt aaaccggacg atgtttgata tggcgcacgc
    17461 caggaaacag catcagcgtc agaaagtaca tcgtcagcgg cggcgctgat tgacgcaccc
    17521 gtcagcaggc gaatttgata cggctttttg gcctcatgct gttcgttagc tctgcgggca
    17581 atcgcggtgg gtagggcttt cggtgaaccc gctggggtaa agccgctaaa tgccaccatg
    17641 tcgttatgct ggataatttc tgccgcttca tcggcggtca tccttgtcca ctgagtttcc
    17701 atttttactc cttcgttcgc agggattaat gaccaacgaa attaggttta cgtttttcga
    17761 ggaaagcgtt catcccttcc tggtaatctt cgctgtcata aacggcgcgg cgcatcccct
    17821 gaatacgttc aaattcatcg gagttcatgg tgtgtgcttc gcccagtaca cgcagctctt
    17881 ctttgataac ggcaatggct aacggcgctt tctcagagat gtggtgcgcc atttgtaagg
    17941 tgaaatcttc cagttcttcc acttccacaa catggttgag gatgccgaca gccagcgcgc
    18001 gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt gacaatgtgg aagcccgcgt
    18061 cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac gccgaggttt acaggtgtca
    18121 ttgagaaggt tgaggtactg gcggcgatga tcagatcgga actcatgatc atttcaaatg
    18181 cgccacccca aacactacct tccaccatcg aaatgatcgg tttcgggaat ttttggatca
    18241 tgcgggtgat ttgacgcaac ggatcatcat aggagagcgg atcgcgaccg ccagacggca
    18301 gttcgtgaat atcgtgacct gcggagaaga ctttggatcc actcggcgcg cgcaaaatga
    18361 tacagcgaat ttccggtcgg ttgagatcgc ttaatgcctg cataagatca tcaataaaga
    18421 ctttacttaa ggcattaagt ttgcggccat agttaaactc aatgaccgcc actttgttga
    18481 tagtgacaac gttcacatac tgataagaca taaaaattcc tttaatcaaa atattgcgtc
    18541 tggataaatt cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt gtttttgacc
    18601 gctaaaagcg tctggcggta atagcgatcg aaatcttcat tcgcgaacag gtgattcagt
    18661 acttcttctt cggtttgttt acgcagccat tccaccgatt gttgttgccg cacttgctgt
    18721 aaacgaccac tggcagttag cgcggttttg aagtcgatga tggcgtgcca gatctcatcg
    18781 attccacgtt tttccagtgc gctacaagtc agaacccgtg gctgccattc gtcgtattta
    18841 cgtcgcagaa tatgcagggc actctcgtac atatgccggg caatggcgac attggtatgg
    18901 ttatcgccat cgtctttgtt gataacgatc agatcagcca cttccatcag ccctttttta
    18961 atgccctgca gatcatcgcc gccaccggca atttgcaacg agataaaaca gtccaccatg
    19021 cgggcgactt ccgtttccga ctgcccgacg ccaaccgttt cgacaatcac tacgtcataa
    19081 cccgccgctt cgcataacag cattaattcc cgcgctcgct gactggcacc gcccagatga
    19141 ccggaggatg gtaccgggcg aataaacgcc gtctcggcac gcgccaggtc attcatgcgg
    19201 gttttatccc cgagaatgct accgccagtg accggactgc tgggatcgac cgcaataacc
    19261 gcaactttta atccatcacg aatcaacaac atgccaaagg cttcaagaaa ggtacttttc
    19321 cccgcgccgg gtgtgccggt aacgcccagt cgcagggcgt taccgcagta cggcataatg
    19381 gcatcaagca gttgcgtact tagcgcctga tgacgcgggt gacggctttc caccagcgtc
    19441 atggcctggg cgagtgtggc acgctcaccc tgacgtaagc ggcgaatact ttctgccagc
    19501 gtggcttcat taatcatgat gctggcttat cagattcagt acgtcgcgca cactgtcgag
    19561 cataggtgta cctggaccat aaatcgccgc cacgccgcgc tcttgcagga aggcgtaatc
    19621 ctgcggtgga atgacgccac cggcgactac gcagatatct tcgcgtcccc attttttcag
    19681 cgcttcgacc agttccggga tcagcgtttt atgaccggca gccagtgagg atgcgcccac
    19741 tacgtgaacg tcattttcta ctgccaggcg ggcgatctct tcaggtgtag agaacatcgg
    19801 gcttaaatct acgtcgaaac cgagatcgga ataggcgctg gcgatcactt ttgcgccgcg
    19861 atcgtgccca tcctggccca ttttggcgat cagaatgcgc gggcgacgac cattgtcggc
    19921 aaggaactgc tccgtttgcg caacaatggc atcgaactcg gaggccgatt tctcagattg
    19981 atgatagctt tgcgcaatta cgccggtaac acactggctt ggcaccagat aacggtcgaa
    20041 agcggcttcc agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag cggcattaac
    20101 agcggcagcc agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt tcaacgcggc
    20161 ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa tttgctcgtt
    20221 acgcaccatc acgttgtcga tctcaagtac atcggtttcg tcttcgtgat ccagtttgta
    20281 cttgttgaca ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt cgcgcgctga
    20341 ggcctcttcg atcattcgtt ttggcagacc tgcttcgatc gctttcgcca tgccaccggc
    20401 ttcgtcgatc tgttggataa taactctggc ttgtttgacg atttgatcgg tcagcgactc
    20461 aatgtaatag gatccggcca gtggatcgac ggtgcggcag agttctgatt cttcctggat
    20521 gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac caagcgcttc
    20581 gtcaaaggcg ttggtatgca gtgactgagt accgcccagc gtcgcagcca gtgcttcaat
    20641 ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc aggctccagc ctgaggtctg
    20701 gcagtgggta cgcagcgcca gtgatttcgg gtcctgtgcg ccaaatccac tgaccgcttc
    20761 gctccataaa taacgtgccg cacgcaacat ggcgacgttc ataaacagat ccatgccgat
    20821 gccgaagaag aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg cagagattgc
    20881 tgctttgatg tactcaatcc catcagcgag cgtaaatgct acctgctgca cgcagttggc
    20941 acccgcttca cccatgtggt aaccgctgat actgatggta ttaaatcgcg gcatgttgcc
    21001 ggaacaccag gcgatgatgt cggcgataat gcgcattgac ggttttggcg ggtaaatata
    21061 ggtgttgcgg cagaggtact ctttgaggat atcgttctga atggtgccgg tcagtttatc
    21121 aggtgtaaca ccttgctctt ccgcggcgac gatataaaac gccagtactg gtagcactgc
    21181 gccattcatg gtcatcgaaa ccgacatttt atccagcggg atctggtcga acaggacttt
    21241 catatcttcc acggtgtcga tagcgacgcc cgctttgccg acgtcgcccg ccacgcgcgg
    21301 gttatcggag tcgtagcctc ggtgggtggc aaggtcaaac gcaacggaaa gacctttttg
    21361 cccggcggcc aggttacggc gataaaaagc gttggactct tttgctgttg aaaaaccagc
    21421 atactgacgg atggtccacg gttgggcggt atacatagtg gcacgcgggc cacgaacgta
    21481 gggcggcaaa ccaggaaggg tacctgtcac ctccagatta tcgagatcgg cttcggtata
    21541 cagcggcttg atggcgatcc cttccgcggt ttgctgaacc agcgagtcga cagttttctc
    21601 ccgacggctc aattccttgt tggcaagctg ttgccactcc tgcacgttag acattatttg
    21661 ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcctac ggtgagcttt ttacgtgggt
    21721 ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt gccgcctgag atttgtctat
    21781 tttgtgatgt gattcaagga atacaaaaag aagtgaggac aacgcctgat atgtgctatc
    21841 aggcattatt tgatggatta atcctgacga aggactttgt gaccataatc gagtaacgca
    21901 tcagtgactt tacgcatcat gcggctttca ggagcaaagc ggtgccagta gagcatccgt
    21961 cgttgaaata gcccaggcgt taagtcaatc agttcaccgc tggccagctc tttctcgatt
    22021 tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag caagttgcac gaacgcttct
    22081 gaagaattaa cgatatggca gggtacgctg ccaggaggca gatcgaagtt ttgctgcaaa
    22141 aaggcctggt gcatatcgtc aagatggtca aacgcgacca ctggcgcttt cagtaatgcc
    22201 gaacgcgtta cgccgttagg gaaatatttt tcggcaaacg gttttgagct gacgaacaga
    22261 tagtcgagcg caccaagttt atcgacaaga caactcggca gcgcctgatg ttgaatactc
    22321 accgcgccga ccacttcgcc gcgacgcaga cgttcctgag tgcgggtttc atcttctacc
    22381 tgcaagttga ggcggatagg cgaatcagcc aacacaggag ccagtgccgg aagcaaccac
    22441 gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca gcggagtcga accggtttgt
    22501 tcatcgccca gccactcttc ttccagcaac tccacctggc gcagcagtgc cagcagtttt
    22561 tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca acagcggctg cccgaacata
    22621 ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg taatgcacag cttttgtgcc
    22681 gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg cctgtaatgt tctgtagtcc
    22741 gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact atgacataat
    22801 tttgcgttgg gtacagagca tttaacgcat acaaaagcgg tcgtaatgga agttttggct
    22861 ggcaatccca ttttcatata accctttgct gagcgctttg gcaaaactct gtgggccgca
    22921 aaaccagaaa tgggtgtcac ctttatttgc tattttatca gtaagatagt gcgactgtag
    22981 gcgctcacct ttatgcgcat caagcagatg cagtgtgaca ctggcctggg cacaaagtga
    23041 ggtaagttta tcaacatacc atgcatcctc gcttcggctg acgcaatacc agagatccac
    23101 aggaacacgg gacgcgccat gctggttcag atactcaagc tgggcgataa aaggggtaat
    23161 gccgatgcct cctgctatcc agacctggcg ggagcaaggg agagtaaaat caaactttcc
    23221 ccatggccct tcaacgaggg cggactggcc gatttgtatc gaggatgcca gagtcgaggt
    23281 ataatcccct aaatttttga tggcaaagcg caacgtgttc ttctctttta catatgaagc
    23341 gatagtaaaa gggtgagatt ctttactgtg tgcgaacgtt aagaatgcaa actgccccgg
    23401 acaatagtcg atgggatgtt ccatctccag tatgacttcg gtaatttgtc ccggatggtt
    23461 aacaatgctt ttaacggtgg cctgatgctt gcttttatta ccgtttaatt tcagcagcga
    23521 aatgaaagcc acaaagacgc ctgggatagt aattaaaatt aataaatacg cagcaggagt
    23581 ttcccaccaa tatgatttaa acagtgcagt aaatacatga aaagcaattg ccagataaaa
    23641 aacggggaac aaacggtgaa caagatgaaa taaatgatag ggaacttttt tgcttagaga
    23701 cacgaaaacc atcgctatca taataaacat tgcacattca cccgctaaca atcccgcatg
    23761 aagccaattt tcttgtactg gcgtcaggtt aatatcaacg attttaccag ggtgagtaag
    23821 ccagccaaag gcgacgaata actggggtaa tttttcatcc agccagtggg cgaaagcaaa
    23881 agcgatggcg catattcctg cccacttatg taatcgccat gctttatcca gaccgcccag
    23941 cactttatta agccatgatg aacgcagcga taaacacatc gtcagcgtca tcagacacca
    24001 cgcaagggtt cccgtaaaaa agagcgtgtt ttgtcgaatg aaccatatta gcgcgattgg
    24061 cttttcacga aaaatatcgc tggacgacaa aaaaataata catgcgacaa agcaaaccaa
    24121 cgtacctgta agattagctt ttttcatttt ttatttcatt ttggcagcgg agcatagaac
    24181 agttccatgc tcccgttaaa tagagtgatt atttatagaa aatcacagtg gcgtga
//
LOCUS       DAZGBC010000070        24147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGBC010000070 DAZGBC010000000
VERSION     DAZGBC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.070"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..237)
                     /locus_tag="ACHK1J_002656"
     CDS             complement(<1..237)
                     /locus_tag="ACHK1J_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO7911903.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTE"
     gene            complement(414..1325)
                     /gene="gsiD"
                     /locus_tag="ACHK1J_002657"
     CDS             complement(414..1325)
                     /gene="gsiD"
                     /locus_tag="ACHK1J_002657"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO7911904.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIMVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1328..2248)
                     /gene="gsiC"
                     /locus_tag="ACHK1J_002658"
     CDS             complement(1328..2248)
                     /gene="gsiC"
                     /locus_tag="ACHK1J_002658"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO7911905.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2266..3804)
                     /gene="gsiB"
                     /locus_tag="ACHK1J_002659"
     CDS             complement(2266..3804)
                     /gene="gsiB"
                     /locus_tag="ACHK1J_002659"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO7911906.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3824..5695)
                     /gene="gsiA"
                     /locus_tag="ACHK1J_002660"
     CDS             complement(3824..5695)
                     /gene="gsiA"
                     /locus_tag="ACHK1J_002660"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO7911907.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQVLRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAPVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5682..6647)
                     /gene="iaaA"
                     /locus_tag="ACHK1J_002661"
     CDS             complement(5682..6647)
                     /gene="iaaA"
                     /locus_tag="ACHK1J_002661"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7911908.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSSHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDAVATQ"
     gene            6851..8086
                     /gene="moeA"
                     /locus_tag="ACHK1J_002662"
     CDS             6851..8086
                     /gene="moeA"
                     /locus_tag="ACHK1J_002662"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO7911909.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8086..8835
                     /gene="moeB"
                     /locus_tag="ACHK1J_002663"
     CDS             8086..8835
                     /gene="moeB"
                     /locus_tag="ACHK1J_002663"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO7911910.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8911..9573)
                     /gene="fsa"
                     /locus_tag="ACHK1J_002664"
     CDS             complement(8911..9573)
                     /gene="fsa"
                     /locus_tag="ACHK1J_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7911911.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMAITAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9704..10603
                     /gene="ybiY"
                     /locus_tag="ACHK1J_002665"
     CDS             9704..10603
                     /gene="ybiY"
                     /locus_tag="ACHK1J_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO7911912.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10609..13041
                     /gene="ybiW"
                     /locus_tag="ACHK1J_002666"
     CDS             10609..13041
                     /gene="ybiW"
                     /locus_tag="ACHK1J_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO7911913.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRFHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13187..14002
                     /gene="ybiV"
                     /locus_tag="ACHK1J_002667"
     CDS             13187..14002
                     /gene="ybiV"
                     /locus_tag="ACHK1J_002667"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO7911914.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14149..15414
                     /gene="ybiU"
                     /locus_tag="ACHK1J_002668"
     CDS             14149..15414
                     /gene="ybiU"
                     /locus_tag="ACHK1J_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO7911915.1"
                     /translation="MAPTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAG
                     DSVWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15655..17247)
                     /gene="ybiT"
                     /locus_tag="ACHK1J_002669"
     CDS             complement(15655..17247)
                     /gene="ybiT"
                     /locus_tag="ACHK1J_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO7911916.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17466..18386
                     /gene="ldtB"
                     /locus_tag="ACHK1J_002670"
     CDS             17466..18386
                     /gene="ldtB"
                     /locus_tag="ACHK1J_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7911917.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18444..19562)
                     /gene="ybiR"
                     /locus_tag="ACHK1J_002671"
     CDS             complement(18444..19562)
                     /gene="ybiR"
                     /locus_tag="ACHK1J_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO7911918.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLIQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19559..20026)
                     /gene="mntR"
                     /locus_tag="ACHK1J_002672"
     CDS             complement(19559..20026)
                     /gene="mntR"
                     /locus_tag="ACHK1J_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO7911919.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20212..20340
                     /gene="mntS"
                     /locus_tag="ACHK1J_002673"
     CDS             20212..20340
                     /gene="mntS"
                     /locus_tag="ACHK1J_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO7911920.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20612..22195
                     /gene="opgE"
                     /locus_tag="ACHK1J_002674"
     CDS             20612..22195
                     /gene="opgE"
                     /locus_tag="ACHK1J_002674"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7911921.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKDGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22245..22760)
                     /gene="ompX"
                     /locus_tag="ACHK1J_002675"
     CDS             complement(22245..22760)
                     /gene="ompX"
                     /locus_tag="ACHK1J_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO7911922.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22813..22878)
                     /locus_tag="ACHK1J_002676"
     CDS             complement(22813..22878)
                     /locus_tag="ACHK1J_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7911923.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23113..24000
                     /gene="rhtA"
                     /locus_tag="ACHK1J_002677"
     CDS             23113..24000
                     /gene="rhtA"
                     /locus_tag="ACHK1J_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO7911924.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5917 a         6566 c         5972 g         5692 t
ORIGIN      
        1 ctctgtattt tttaataaat acagtgagtc ctttaatgtc agggccgaac gttcaatatc
       61 tttaaagaaa tagccaacgt ttttctgaat aagttcaaag cggctatcgg cacgttttac
      121 cgcgcgttcc gataccagtt gatagttaat gatgaaaaaa aggccaataa atgacagtgc
      181 tgccaataaa aataaaatta tcagccttat ctttatcttt tcgtataaac tcagcatgtt
      241 aatacattta acaaaataac tgtctgaacg ctgtgccgct gcaaaaacag tcccttgctg
      301 cggcttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
      361 gggcaataat ttcctgtgag gtgattactc tttcaagcaa tattcaaacg taactatcct
      421 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtaca
      481 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
      541 cgagcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
      601 aatgacaggc tggcggcaga gataatcgaa gtgccaatgc gcatggtgaa aaacaccacg
      661 atagacgaga cggtcccagg caagatatga cgcaataaaa tggtcatatc gctggcacca
      721 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
      781 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcaa taatcacgtt agcaatgccg
      841 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
      901 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc cagcaagccc
      961 agcaacgtcc ctatcgccgc accgataaac acggcaaaca cgcccgcagc cagcgagatt
     1021 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     1081 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     1141 ggagcgatcc agcgtgcaaa aatggccacc ataatcaata aaataacgaa taatgcggcg
     1201 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     1261 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     1321 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     1381 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     1441 cgcgcatttc tacggagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     1501 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
     1561 ggatcatcgc gttacgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
     1621 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaacgacgcg cgggtaaagc
     1681 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
     1741 aactgtctgc tcccacggta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
     1801 gcatccccag agcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
     1861 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
     1921 ccgcccagac catactggtt atggtcagcc acagcgttgg cataaagcgg ctggcaatct
     1981 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
     2041 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
     2101 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
     2161 gcatatgcac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
     2221 gtaatcgttt gataacgtaa ttaagcattc cactccctgc gttgcttatt gcaaatccgc
     2281 gtcttcaaag ctgaagccgg tgtctggcat gatccaaaaa ccggtcaggt ttttactgtg
     2341 tgccgacacc agtttttcta ccaccagcgg gatccacggc gactcttgcc agatgatatc
     2401 ctgcgccgcc ttatacaagc gggtcttttc cgccggatca ttagttttca gtgcctgagc
     2461 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
     2521 gttctgcgag gcaaacagcg gcgatagtgc ccagtccgct tcgccggttg aagccgacca
     2581 gccagtgtag aacatccgca cgccgctctc tttttgccct ttaccttcaa cttctgccgc
     2641 ccgctgtccg gcatccatcg cagtcacctg ggctttaatc ccgacctgcg ctaactgctg
     2701 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
     2761 attgaaacca ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
     2821 aggccacggt ttataacttt gcgcgtaggc gatactcggc ggtaccacac cagtagctgg
     2881 cgttgcatag cccgcgaagg ccactttcac cagcgccgga cggttaatgg cgtaattcag
     2941 cgcctcacgg accttcgggt tatcgaatgg cttttgcgtc acgttcatac tgatataacg
     3001 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgcggc
     3061 ctgctcgtaa ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
     3121 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
     3181 ccagtaaccc gcgaattttt tcaccttcac aaaatcagtc tgattccagg tatccagttc
     3241 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgc
     3301 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
     3361 tttgagggta atctttaccg ttgtcggatc gatcgcttcc gttttagcaa tattcttata
     3421 caggttatag cgtttaagat gatttgccgg atcgctggcc cggtccagat tcgctttcac
     3481 cgctgcggcg ttgaaatcgg tgccatcctg gaatttaatg ccttcccgca atttcacggt
     3541 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
     3601 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
     3661 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
     3721 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
     3781 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
     3841 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
     3901 gtgcaacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
     3961 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
     4021 aaggatgctg cgggttttcg aagaccgcgc gccgtggacc aatttcaaca atttgcccga
     4081 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
     4141 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
     4201 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
     4261 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
     4321 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcaaccg
     4381 cttctttacc tggcaataaa ccgtgtacac gcaacggttc gataatcgaa tcaccgatgg
     4441 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcgcggcgca
     4501 atacctgaag tttgccgggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
     4561 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
     4621 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
     4681 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacca
     4741 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
     4801 cctgtttcgc tggatgttca agcgatatca acgggaaacg tcggggataa tctaaccctt
     4861 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
     4921 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
     4981 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
     5041 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
     5101 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
     5161 tcatcactcg ctggcgcatc ccgccagaga gttgatgcgg ataacgtgaa agaatggttt
     5221 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
     5281 gactggcgtt ttgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaataccg
     5341 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatgac
     5401 gcatttgtgc agcgctctgc tcgctaagtt caatcacttc acgactgcgc cgccgcaaca
     5461 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
     5521 cagtcactga cttaccggag ccggattcgc caacaattgc cagcgtctca ccgcgttgca
     5581 ggctaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
     5641 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggcg
     5701 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
     5761 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
     5821 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
     5881 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
     5941 aagacttcgc ccgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
     6001 gcacccacta aggggctatc gccaactcgt ccgggtaatt tattggtcat tccgcccgtg
     6061 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
     6121 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
     6181 gctgccatta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
     6241 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgagctt
     6301 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
     6361 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg cgtttcatca
     6421 cgcgtaaaga cggcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
     6481 gcttccgtca ccacatccag cgcactttcg cccgcttcca gcattttctg cccggtttca
     6541 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
     6601 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
     6661 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
     6721 ttttgaatat agaaagatgg atatggcatg taaaggcagg acccagcgct acgcccgagg
     6781 tggcatacag tctgtggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
     6841 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
     6901 gctttctcgc gtcaccccac tgaccgccca ggaaacgctg ccactggtac agtgttttgg
     6961 tcgtattctg gcgagcgatg tcgtttcgcc gcttgatgtt ccggggtttg ataactccgc
     7021 aatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
     7081 cggtaaatcc tttgccggtc agccgtacca tggtgaatgg cctgcgggta cctgcattcg
     7141 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
     7201 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
     7261 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggga ctcgcctgac
     7321 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
     7381 agtgcgagtg gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
     7441 cgacggccaa atctacgata ccaaccgtct cgccgtacac ctgatgttag aacagctggg
     7501 ttgcgaggta attaacttag ggattatccg cgatgatccc catgccctgc gcgccgcatt
     7561 tattgaagcc gacagccagg cggatgtggt gatcagttcc ggcggtgttt cagtgggtga
     7621 agcggattac accaaaacga ttcttgaaga gctgggggag atcgccttct ggaagctggc
     7681 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
     7741 gccgggcaac ccagtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
     7801 aaaactaagc ggtaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
     7861 ccgcctgaaa aaaacgccag gtcgccttga tttccagcgc ggcgtgctgc aacgcaacgc
     7921 cgatggcgaa ctggaagtga cgaccactgg acatcagggt tcacatatat ttagctcctt
     7981 tagcctcggc aactgcttta tcgtactgga acgcgatcgc ggcaatgtgg atgtgggcga
     8041 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
     8101 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
     8161 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
     8221 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgactcg
     8281 gtttcgctct cgaatctgca acgccagacg ctgcacagtg atgccacggt cgggcaaccg
     8341 aaggtggaat ccgcccgtga cgccctggcg cggatcaacc cacatatcgc gattacgcca
     8401 gtcaatgcac tgctggatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
     8461 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggatg ttttgccgcg
     8521 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag gtcaaatcac ggtcttcact
     8581 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
     8641 acctgcgtag aagcaggcgt gatggcacca ttgatcggcg taattggttc gttgcaggca
     8701 atggaagcga tcaagctgct ggcaggttat ggcaaaccgg ccagcggaaa aatcgtgatg
     8761 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgaa
     8821 gtgtgcgggc agtaattgcc acctccaggc ctgataagac gcggcactcc gccacgtcat
     8881 cagcaattta tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctccctg
     8941 ccagtcctgc tcaaacttcg ccacagcggc atcaaccgcc ggatagctaa tcatctgttg
     9001 tgccacatcc agtggcagag taattgattc acatcctgcc agtaagcagt ccagcgcctg
     9061 acgcggggtt ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca ttttcaataa
     9121 ctggtgtaag tcggtcacag tctgaatgcc gctaccgccc tgagcatcaa tacgattaac
     9181 gtaaggcgca acatattccg cacctgccag cgccgacagc aacccttgtg ccgcgccata
     9241 taccgcggtt cccagcgtcg gaatcccttc cgcttttaac atcttaatcg ctgccagccc
     9301 ctcggcggtc accggaactt tcaccacgat atccgcaata atagaacgca atttacgtgc
     9361 gtcattcacc atcccttcag cagtgatagc cattacctgg gcaaacagac gcccctgacc
     9421 gcccatcgct tcatgaagtt ccggaagcac aacctccagc ggttttttac ccgcggcgat
     9481 aatgcttggg ttagtggtca cgcccgccag cggaaaaata cgtgacagcg ccttcaccgc
     9541 aacaacgtct gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc
     9601 tgaatgtaga ctatcacggg aaaactctct gaacagttga cgcacatcaa tctaactttc
     9661 attcgaaagt attttaatgt ttgaacgaaa cagagaggca actatgattt tcaatattca
     9721 gcgctactcg acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc
     9781 gctgggctgc cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg atctgctgta
     9841 tgacgcacga ctgtgtctgg aaggctgcga gctgtgcgct aaggcagcgc cggaagtgat
     9901 tgagcgcgcg ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcatctgac
     9961 ggcgttaacc gactgctgcc cgacacaggc attaaccgtg tgtggtgaag tgaaaagcgt
    10021 tgaggagatc atggcgaccg ttttgcgcga taaaccgttt tacgatcgca gcggcggagg
    10081 cttaacgctt tcgggtggtg aaccctttat gcagccggaa atggcgacgg cgctactgca
    10141 agccagccac gaggccggca ttcatactgc ggtagaaacc tgtctgcatg tgccgtggaa
    10201 atatatcgcc ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc
    10261 cgacgcgccg tttaaacagt ggaccgacgg taacgccgcc agagtgctgg ataacctgaa
    10321 aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc agggctttaa
    10381 tgccgacgaa acctctgtaa aagccattac cgattttgcc gccgacgagc tgcacgttgg
    10441 cgaaattcat ttcctgccct accacacgct gggcatcaac aaatatcact tacttaatct
    10501 gccctatgac gccccggaaa aaccgcttga tgcgccagaa ctgctcgact ttgcccagca
    10561 gtatgcctgc cagaaagggt taaccgcgac cttacgagga taacaaccat gaccacactg
    10621 aaactggaca cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg
    10681 aaaccgccgg tttgtaccga gcgcgcgcag cactataccg agatgtatca acaacatctc
    10741 gataagccga tccctgtacg tcgcgcgctg gcgctggcac atcacctggc gaatcgcact
    10801 atctggatca agcacgatga gttgatcatc ggcaaccagg caagcgaagt tcgcgccgcg
    10861 ccgatcttcc cggaatatac tgtctcgtgg atcgaaaaag agattgatga tctggcagat
    10921 cgtcccggtg ctggctttgc ggtgagcgaa gagaacaaac gcgttctgca tgaagtgtgc
    10981 ccgtggtggc gcggtcagac cgtacaggat cgctgctacg gcatgtttac cgatgagcaa
    11041 aaaggtctgc tggcgaccgg catcattaaa gcggaaggca atatgacctc cggcgatgcg
    11101 cacctggcgg tcaatttccc gctgctgctg gaaaaagggc ttgatggtct gcgcgagaaa
    11161 gtagcggaac gtcgctcgcg catcaacctg acggtgctgg aagatttaca cggcgagcag
    11221 ttcctgaaag caattgatat cgtgctggtg gcagtcagtg aacacattga acgtttcgct
    11281 gccctggcgc gtgaaatggc cgcgaccgaa acccgcgaaa gccgtcgcga tgaactgctg
    11341 gcgatggcag aaaactgcga tcttatcgcc caccagccgc cgcagacgtt ctggcaggcg
    11401 ctgcaactgt gttacttcat ccagttgata ttgcagatcg aatctaacgg tcactcggta
    11461 tcgtttggtc gtatggacca gtatctctac ccttactatc gccgcgacgt tgaactcaac
    11521 cagacactgg atcgcgaaca cgccatcgaa ttgctgcata gctgctggct gaaactgctg
    11581 gaagtgaaca aaatccgctc cggctcacac tcaaaagcct ctgcgggaag tccgctgtat
    11641 cagaacgtca ccattggcgg gcaaaatctg gttgatggtc aaccaatgga cgcggtgaat
    11701 cccctctctt acgcgatcct tgaatcctgc ggtcgcctgc gttccactca gcctaacctc
    11761 agcgtgcgtt tccatgcagg aatgagtaac gatttcctcg acgcctgcgt acaggtgatc
    11821 cgttgcggct tcgggatgcc ggcgttcaac aacgacgaaa tcgtcatccc ggaatttatt
    11881 aaactcggta ttgaaccgca ggacgcttac gactatgcag cgattggttg tatcgaaacc
    11941 gccgtcggtg gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc
    12001 gtgatgctgg cggcgctgga aggcgggcgt gatgccacca gcggcaaagt gttcctgcca
    12061 caagaaaaag cgttgtcggc aggtaacttc aacaacttcg atgaagtgat ggacgcgtgg
    12121 gatacgcaaa tccgttacta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgacacc
    12181 atgctggaag agaacgtgca cgatattctc tgctcggcgc tggtggatga ctgtattgag
    12241 cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtt
    12301 ggcattgcca acctcggcaa cagcctggcg gcagtgaaga aactggtgtt tgaacagggc
    12361 gcgattggtc agcaacagct ggctgccgca ctggcggatg acttcgatgg cctgactcat
    12421 gagcaactgc gtcagcggct gattaacggc gcgccgaagt acggcaacga cgatgatacc
    12481 gtcgatacgc tgctggctcg cgcttatcag acctatatcg acgaactgaa acagtaccat
    12541 aacccgcgct acggtcgtgg tccggttggc ggcaactatt acgcgggtac gtcgtctatc
    12601 tccgctaacg taccgtttgg cgcgcagact atggcaacgc cggacggacg taaagcgcat
    12661 accccgctgg cagaaggcgc aagcccggct tccggtactg atcatcttgg ccctactgcg
    12721 gtcattggct cggtgggtaa actgcctacg gcagcgattc tcggcggcgt gttgctcaac
    12781 cagaaactga atccagccac actggagaac gaatctgaca agcagaaact gatgatcctg
    12841 ctgcgcacct tcttcgaggt gcataaaggc tggcatattc agtacaacat cgtttcccgc
    12901 gaaacattgc tggaggcgaa aaaacatccg gatcaatacc gcgatctggt agtgcgtgtc
    12961 gcgggctatt ccgccttctt caccgcgctc tctccagacg cacaggacga tatcatcgcc
    13021 cgtactgaac atatgctgta atctcctcgg cccggcggcg tcgtcgccgg gctaaatcat
    13081 tcacatcatc aatttcatcc ttactttcat tcgaaatata atttgtgctc tgcgtcacat
    13141 tgttattaca ctgttacggt ttcattttcg agccaggagc acacctatga gcgtaaaagt
    13201 tatcgtcaca gacatggacg gtacttttct taacgacgcc aaaacgtaca accaaccacg
    13261 ttttatggcg caatatcagg aactgaaaaa gcgcggcatt aagttcgttg tcgccagcgg
    13321 taatcagtat taccagctta tttcgttctt tcctgagcta aaggatgaga tctcttttgt
    13381 cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg gcgaactgac
    13441 ccgacatgaa tcgcggattg tgattggcga gttgctaaaa gataagcaac tcaattttgt
    13501 cgcctgcggt ctgcaaagtg catatgtcag cgaaaacgcc cccgaagcat tcgtcgcact
    13561 gatggcaaaa cactaccatc gcctgaaacc tgtaaaagat tatcaggaga ttgacgacgt
    13621 actgttcaag ttttcgctca acctgccgga tgaacaaatc ccgttagtga tcgacaaact
    13681 gcacatagcg ctggatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgacct
    13741 gattattccc ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct
    13801 gtcaccgcaa aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat
    13861 ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta
    13921 cgctaccgat gataataatc atgaaggcgc gctgaatgtg attcaggctg tgctggataa
    13981 cacatcccct tttaacagct gacctctcta ccggagccat gcgctccggt tttcccttct
    14041 ttttacatcc aacacatcaa atcattaaac ttgcattaac ttatttataa cttatattgt
    14101 cacatgcaaa tcagcacagt ttcgaatgaa agttaatgcg aggtggttat ggctcctact
    14161 tttaccagcg acacattgcc tgccgatcac aaagcagcta tccgtcagat gaagcacgcg
    14221 ctgcgggcgc agcttggcga cgtccagcag atctttaatc agctaagcga tgacattgcc
    14281 acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg
    14341 tcttatgccg atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt
    14401 cgcggttgtg cggtgataaa aggccatttc ccccgcgaac aagcgctagg ctgggatcag
    14461 tcgatgctgg actatctgga ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat
    14521 aatttcttcg ggacgctcag cgcttcacgt cccgagattt accccatcta ctggtcgcag
    14581 gcgcaaatgc aggctcgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt
    14641 ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac
    14701 cctgaccgta tccgccgccg tccgcccgga acgacctcca aaggtcttgg agcgcatacc
    14761 gactccgggg cactggaacg ctggctgctt ccagcgtatc agcacgtttt cgctaacgtc
    14821 tttaatggca atctggcgaa atacgatccc tggcatgcgg cacatcgtac ggaagttgaa
    14881 gagtacacgg tagataacac tacaaaatgt tcggtgttcc gcacgtttca aggctggacg
    14941 gcgctctctg atatgttatc gggtcaggga ttgttacatg tggtgcctat cccggaagcg
    15001 atggcgtatg ttttattgcg cccgctgctg gatgacgtac cggacgatga actatgcggg
    15061 gttgcacctg gcaaagttct gcccatttca gagaaatggc atccactact gcttaaagcg
    15121 ctgacttcca ttcccgcgat aacagcaggg gattccgtct ggtggcactg cgatgtgatt
    15181 cactccgtcg cgccagtaga caatcaacag ggttggggca acgtgatgta tatccctgct
    15241 gcgccaatgt gcgagaaaaa tcgggcttat gcacacaaag tcaaaatggc gctggaaaaa
    15301 ggcgcatcgc cgggcgactt cccgcgcgag gactatgaaa caaactggga aggacgcttt
    15361 acgcttgccg acctcaacat tcacggtaag cgcgcactgg gcatggatgt ttgagaatta
    15421 atggaagtga attgatgtgg gattaagaaa atcgattcga cgttactcag attgctgaca
    15481 agtgcgcttt cttaatgcct gatgcggcgt taacgcctta tctggcctac aaagtcctgc
    15541 agattcaata tattacaggt actgcgtagg cctgataagc gtagcgcatc aggctatttt
    15601 gcatttgtca tcagccgccg tcaccccgac agtatcgggg tgacggataa taacttactc
    15661 gatcccttta ctacgcaggt aatcttcgta attaccgcta aagtcgatca cgcgttccgg
    15721 ggtgatttcc agaatgcggg tcgccaggga gcttacgaac tcacggtcgt gtgaaacaaa
    15781 gatcagcgtg ccctgataca gttccagcgc catgttcagt gactcgatgg attccatatc
    15841 caggtggttg gtcggttcgt ccatgatcag aatgttcggc ttctgcatca ttaacttacc
    15901 aaaaagcatc cgcccttttt ctccaccgga aagcacttta gctggctttt tgatgtcgtc
    15961 ctggctgaac agcaaacgac cgagaatgct gcgtaccgcc tgctcgtcat cgccttcctg
    16021 cttccactgg ctcatccatt cgaacacggt cagatcattt tcaaactcat attcgtggtc
    16081 ctgagcatag taaccaatgc gcgcgttttc agaccatttt acggtgccgc tgtccggttg
    16141 cagatcaccc accagcgttt tcagcagcgt tgatttaccg acgccgttgg tacccagtac
    16201 cgcaagtttt tcaccgacct ccagcagcag gttgagattt ttaaacagtg gaccgttatc
    16261 aaaccctttg gtcagacctt ccacttccag cgcgttacgg aacagtttct tatcctgttc
    16321 aaaacggatg aacgggttct gacggctgga ggctttcacc tcttccagtt tgattttatc
    16381 aatctggcgc gcgcgcgaag ttgcctggcg agatttcgag gcgttggcgc taaagcggct
    16441 aacgaaagat tgcagctcag caatctgcgc tttcttcttg gcgttatcag ccagcagacg
    16501 ttcacgcgcc tgggtcgccg ccgtcatgta ctcatcgtag ttgcccggat aaacgcgcag
    16561 ctcgccgtaa tccagatccg ccatgtgggt acaaaccatg ttaaggaagt gacggtcgtg
    16621 cgagatgatg atcatggtgc tgtcacgctc gttcagcacc tgttccagcc agcgaatggt
    16681 gtcgatgtcg aggttgttgg tcggttcgtc gagcaggaga atatccggat cagcaaacag
    16741 cgcctgcgcc agcagcacac gcagcttcca gccaggagca acttcactca tcgggccgta
    16801 gtgctgttcc actggaattc ccacgccaag caacaattca ccggcgcgag cttccgcaga
    16861 gtaaccgtcc atttcgccgt atttaacttc cagatcggcc actttatagc cgtcttcttc
    16921 actcatttcc ggcaaagcat agatgcggtc gcgctcctgc ttcacttccc acaactcttt
    16981 atgccccatg atcaccgtat ccagcacagt gaactcttca aaggcaaact gatcctgacg
    17041 cagcttacca atgcgctcgt tgggatcgag ggaaacgtta cccaacgtcg gctctaagtc
    17101 gccgccgagg atcttcataa aggtggattt accactaccg ttcgcgccaa tcaggccgta
    17161 acggttgccg ccgccaaatt tgacggaaat gttttcaaac aacggcttac tgccgaactg
    17221 catggtgacg ttactggaaa ctaacacagg cgtatcctga aaagagatat gacaaaccgc
    17281 gcattatgcc ataagtctga aagaagatcc tgctcctcgc ttaaggtcta cactcaagcc
    17341 aaaggcaaaa aatgtgattt cgtacacatc tgatttcact gtgagctgga atgaacttat
    17401 aatgcgcttc caatactcta atattctcaa cccaatggcc tgccaggcac aaaatctcgc
    17461 ttaacatgaa tatgaaattg aaaacattat tcgcagcggc cttcgctgtt gtcggctttt
    17521 gcagtaccgc ctctgcggta acttatcctc tgccaaccga cgggagtcgc ctggttggtc
    17581 agaatcaggt gatcaccatt cctgaaggta acactcagcc gctggagtat tttgccgcgg
    17641 agtaccagat ggggctttcc aatatgatgg aagcgaaccc gggtgtggat accttcctgc
    17701 cgaaaggcgg tactgtactg aacattccgc agcagctgat cctgccggat accgttcatg
    17761 aaggcatcgt cattaacagt gcagagatgc gtctgtatta ctatccgaaa gggaccaaca
    17821 ccgttatcgt gctgccgatc ggcattggtc agttaggcaa agatacgcct atcaactgga
    17881 ccaccaaagt tgagcgtaag aaagcaggcc cgacctggac gccgaccgcc aaaatgcacg
    17941 cagagtaccg cgctgcgggc gaaccgcttc cggctgtcgt tccggcaggt ccggataacc
    18001 cgatggggct gtatgcactc tacatcggtc gcctgtatgc tatccatggc accaacgcca
    18061 acttcggtat cggcctgcgt gtaagtcatg gttgtgtgcg tctgcgtaac gaagacatca
    18121 aattcctgtt cgagaaagta ccggtcggta cccgcgtaca gtttattgat gagccggtaa
    18181 aagcgaccac cgagccagac ggcagccgtt atattgaagt ccataatccg ctgtctacca
    18241 ccgaagccca gtttgaaggt caggaaattg tgccaattac cctgacgaag agcgtgcaga
    18301 cagtgaccgg tcagccagat gttgaccagg ttgttcttga tgaagcgatt aaaaaccgct
    18361 ccgggatgcc ggttcgtctg aattaatcgt caaaacgtaa aacaaaggcg gactcataat
    18421 ccgccttttt tatttgccag accttaccag gccgggatca taaccaacaa aacatatccc
    18481 accagcgccg cccacaacag catcggtatt gaatagagat ggaaacgcca ccagatgcgg
    18541 cgatcgttcg ccatacgtag cgcaatcaaa tttgccagcg atccgggtaa taacccaaag
    18601 ccacctacgt ttaccgccca tgccagtaat aaagacggcg gcacatagtt cagcaacaat
    18661 atggtactcg gcacgttact gatcacctgc gataaaccga ttgccgttaa ccataacccg
    18721 ggttcagata aatgactcac gttacccaac acgccttgca acgctggaag ctggatcagt
    18781 aaatggacgt cgataaacat cgccataaac accagcagca gcgtccagtc cacactcaac
    18841 accacgcgac gtgccagcag cgcaaagccc accgccacaa tcaccagtcc ccacagctct
    18901 tgttttaact ccagcgccgt cagaaagacg atatacagtc ccagacaact ccacaccagc
    18961 cgcggtttcc actccggcgt ttgcaccccc gtatggtatt gcagcgcctt tccagggaaa
    19021 caacaccagc acaacagcag gagtgtcagc atcattgcac cagccagcgg tgccatttgg
    19081 gcaataaatc cggcaaacga aagaccagaa cgtccccaga taagaatatt ttgcgggttg
    19141 ccaattggcg tcagtagcga accagcattg actgccagcg cctcaaaaat aatcagccga
    19201 ttaaccggga tctcacacag tctttttagc gtgatagtca gcggaacaac aataaacagc
    19261 gcgacatcgt tggtcagaaa ggtagaaagc agcgccgccg ccagcaccat aaacatcgcc
    19321 agccgacgct ccgtagcaaa gcggcgcacc attttgcgcc ccagcacatc aaaataaccg
    19381 cttaactcca cacctttggt cagcagcatc aggccgctta atgtgatgat ggtgtgccag
    19441 tcgatagcag caggccagga tttcggtgca aagggcacga aaaagcttaa tccgatacca
    19501 acaagaatta ataactgaaa aaaacgatcc ccttgcagcg tgcgtaaaaa aggcaggctc
    19561 atttggcacc gtgtttctgg gtaaacaaac gaaaggcatc cagcgtctct tcactaacat
    19621 ggtgctccat gccttccgcg tcgcgacggg cgatttccgg actgacgccc aacaccaata
    19681 agaaattttc gactatctga tgtcgctcgc ggctttcctg cgccagcttc tctccttctg
    19741 ccgttaaaaa cacgcctcgc caggggatca tttcaatcag ccccatggtt gccagtcgct
    19801 taagcatttt agccaccgtc ggttgcgaaa ctcccaaacg agcagccatg tctacctgac
    19861 gagcttcccc cacttccctg atcaagtcag aaatcagctc aacgtaatca tcaataagct
    19921 cgcgccgatg cgcctctctg acctggcgga acccttcaac gtgctcttcc acgttcacta
    19981 actgcgtcac tttttttgct gttggcgtac ctgcgcgacg actcattgtg cttcctcatt
    20041 atggtgacgc atacatagcg ttctcatttc gtgcgcacat tgtaaaccag agttgcgaag
    20101 gtacaaaaaa ttaacgtttt agcaatagct atataatata gcctgtgcta tatctgtatg
    20161 taatgcaatc atccctcaag gatcgacggg attagcaagt caggaggtct tatgaatgag
    20221 ttcaagaggt gtatgcgcgt gtttagtcat tctcccttta aagtacggtt aatgctgctc
    20281 tctatgttgt gcgatatggt caacaacaaa ccgcagcaag ataaaccttc cgataaatag
    20341 cggcgtcgcg gtacgccgct tcactcctgc tttcatgcag gcataacgcg ttttggtctg
    20401 aaaaacccca ctttttgtcg gatttgcaat ccccttcgca aaagatttgt tcgtcagtag
    20461 ttgacctgaa cggcggctcg ctctatcttc ttgcagccct gcgtatattg cggctcgcgg
    20521 atgcggaccc ctttcccctc ttcacgcact cttgcaggta ttgacccttg acgccagggt
    20581 aagcacatgg cgtttgttac gatagtggca tatgaattta accctcaaag aatcgcttgt
    20641 tacccgtagc cgggtattta gcccgtggac tgcgttctac tttttacagt cgctattaat
    20701 taacctcggc ttaggttatc ccttcagttt gctctacacc gctgcgttta cggctatttt
    20761 gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggtg tcagttcgct
    20821 ggtggcggct tgctatttcc cttttgctca ggcctacggc gcgcctaatt tcaatacatt
    20881 gctggcattg cactccacca atatggaaga gtcgaccgaa atcctgacga tttttccgtg
    20941 gtacagctac ctggtcggct tatttatttt tgcgctcggc gtaatagcaa tcaggcgaaa
    21001 aaaagagaat gaaaaagcgc gctggaatac cttcgacagc ctgtgtctgg tattcagtgt
    21061 ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg ggtggcgtat ttaaactgaa
    21121 agatactggc tatccggtat ttcgttttgc taaggatgtc atcgtcaata ataacgaggt
    21181 gattgaagag caagaacgga tggcaaaact ttccggaatg aaagatacct ggacggtcac
    21241 tgccgttaag ccgaagtatc agacctatgt ggtggtgatc ggtgaaagcg cgcgtcgcga
    21301 tgccctcggt gcctttggcg gtcactggga caataccccg tttgccagca gcgttaacgg
    21361 tttgatattt gctgactaca ttgccgccag tggctccacg cagaaatcgc ttggcttaac
    21421 gctcaatcgc gttgtcgatg gcaagccaca gtttcaggat aactttgtca ccctggcaaa
    21481 tcgcgcgggc ttccagacct ggtggttttc caaccagggc caaatcggcg aatacgatac
    21541 cgccatcgcc agcatcgcca aacgagcaga tgaagtgtac ttccttaaag atggtaattt
    21601 tgaagcagat aaaaacacca aagacgaagc gttactggat atgaccgctc aagtgctggc
    21661 gcaagagcac tcgcaaccgc agctgattgt tctgcatctg atgggctcgc atccgcaggc
    21721 ctgcgaccgg acacaaggca aatatgaaac ttttgtgcaa tcgaaagaaa cgtcgtgcta
    21781 tctctatact atgacgcaaa cggacgattt actgcgcaag ctgtacgatc agttacgcaa
    21841 cagcggcagc agcttctcgc tggtttactt ttctgaccac ggtctggcct ttaaagagcg
    21901 cggcaaagac gtgcaatacc ttgcccatga tgataaatat cagcaaaatt tccaggtgcc
    21961 ttttatggtc atttccagcg acgataaagc gcatcgtgtg attaaagccc gccgctcagc
    22021 caatgacttc ttaggctttt tctcccagtg gacgggaatt aaagcgaagg aaattaacat
    22081 caaatacccg tttatatctg agaagaaagc cgggccgata tacatcacca acttccagtt
    22141 acagaaggtg gattacaacc atctcggaac cgatattttc gacccgaaac cttaaaaaca
    22201 aaaatccgcc ccgagaggcg gattttttta tatcaccaaa gtgattagaa gcggtaacca
    22261 acaccggcaa tccaggtgcc tacgtcaacg ctacgaatac ggctctgctc gtaagagaag
    22321 tccagagcaa cgttttccat cgggttgaac tgcagaccag caccgtagga gaaaccgtag
    22381 tcgctggtgt cgtgtttgta ggtcgggtat tcagtggtct ggaatttacc ataacccaca
    22441 cccactacac cgtagatgct tgcccagtcg ttaatgcggt aagccggacc agcagtgatg
    22501 ccgtagtact ggtttttgtt gtagtcacca gagcttgcag tacggctttt ctcggtgtaa
    22561 gtgaaagaac cgatcacacc cagcgggctg ttgtcttctt catagcggta tttcaggttg
    22621 aaaccgccca ttttgttcat ttggccctga gcgtcgctct gtgcgtaacc gccagttacg
    22681 gtagaagtcg cagctacgga agtacctgcg gtgaaagcca gaactgcggc cagtgctgaa
    22741 agacatgcaa tttttttcat aaccacctca aatgtgattc aaataagtcc taagttttaa
    22801 atatatcaaa aattaatggg aaactcttcg cgatttgtga tgtctaacgg gccatttcat
    22861 gtaacagaac gtttccatac accgctatcc atctaaattt aaatcacttt ttcagagaac
    22921 tgcgtaagta ttacgcatgt tttccctgtc attcatccag attattccta atcaccagac
    22981 taatgattcc atcaatcctg gcgcatttta gtcaaaacgg gggaaaattt tttcaacaaa
    23041 tgctcaacca gcattgggta tatccagtac actccacgct ttacttaagt ctagatattt
    23101 gtgggagaaa ggatgcctgg ttcattacgt aaaatgccgg tctggttacc aatagtcata
    23161 ttgctcgttg ccatggcgtc tattcagggt ggagcttcgt tagctaagtc actttttcct
    23221 ctggtgggcg caccgggtgt cactgcgctg cgtctggcat taggaacgct gatcctcatc
    23281 gcgttcttta agccatggcg actgcgcttt gccaaagagc aacgattacc gctgttgttt
    23341 tacggcgttt cgctgggggg gatgaattat cttttttatc tttctattca gacggtaccg
    23401 ctgggtattg cggtggcgct ggagttcacc ggaccactgg cggtggcgct gttctcttct
    23461 cgtcgcccgg tagatttcgt ctgggttgtg ctggcggttc ttggtctgtg gttcctgcta
    23521 cctctggggc aagacgtttc ccatgtcgat ttaaccggct gtgcgctggc actgggggcc
    23581 ggggcttgtt gggctattta cattttaagt gggcaacgcg caggagcgga acatggccct
    23641 gcaacggtgg caattggttc gttgattgca gcgttaattt tcgtgccaat tggagcgctt
    23701 caggctggtg aagcactctg gcactggtcg gttattccat tgggtctggc ggtcgctatt
    23761 ctctcgaccg ctctgcctta ttcgctggaa atgattgccc tcacccgttt gccaacacgg
    23821 acatttggta cgctgatgag catggaaccg gcgctggctg ccgtttccgg gatgattttc
    23881 ctcggagaaa cactgacacc catacagctg ctggcgctcg gcgctatcat cgccgcttca
    23941 atgggttcta cgctgacagt acgcaaagag agcaaaataa aagaattaga cattaattaa
    24001 atttacattt ctgcatggtt atgcataacc atgcagaatt tctcgctact tttcctctac
    24061 accgtcttta tatatcgaat tatgcaaaag catatttatt ccgaaaattc ctggcgagca
    24121 aataaataag aattgttctt atcaata
//
LOCUS       DAZGBC010000071        23582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGBC010000071 DAZGBC010000000
VERSION     DAZGBC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.071"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3877)
                     /gene="entF"
                     /locus_tag="ACHK1J_002678"
     CDS             complement(<1..3877)
                     /gene="entF"
                     /locus_tag="ACHK1J_002678"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO7911925.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAAQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHSERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFSSLLGCDVQDVDADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRHMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLN"
     gene            complement(3874..4092)
                     /gene="ybdZ"
                     /locus_tag="ACHK1J_002679"
     CDS             complement(3874..4092)
                     /gene="ybdZ"
                     /locus_tag="ACHK1J_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO7911926.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4095..5297)
                     /gene="fes"
                     /locus_tag="ACHK1J_002680"
     CDS             complement(4095..5297)
                     /gene="fes"
                     /locus_tag="ACHK1J_002680"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO7911927.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLEAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5540..7803
                     /gene="fepA"
                     /locus_tag="ACHK1J_002681"
                     /pseudo
     CDS             5540..7803
                     /gene="fepA"
                     /locus_tag="ACHK1J_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            7978..8598
                     /gene="entD"
                     /locus_tag="ACHK1J_002682"
     CDS             7978..8598
                     /gene="entD"
                     /locus_tag="ACHK1J_002682"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO7911928.1"
                     /translation="MKTTHTSLPFAEHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8720..8872)
                     /gene="hokE"
                     /locus_tag="ACHK1J_002683"
     CDS             complement(8720..8872)
                     /gene="hokE"
                     /locus_tag="ACHK1J_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO7911929.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            8841..9109
                     /locus_tag="ACHK1J_002684"
                     /pseudo
     CDS             8841..9109
                     /locus_tag="ACHK1J_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9324..10442
                     /locus_tag="ACHK1J_002685"
     CDS             9324..10442
                     /locus_tag="ACHK1J_002685"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO7911930.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10508..10756
                     /locus_tag="ACHK1J_002686"
     CDS             10508..10756
                     /locus_tag="ACHK1J_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO7911931.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10821..11189
                     /locus_tag="ACHK1J_002687"
     CDS             10821..11189
                     /locus_tag="ACHK1J_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7911932.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDSQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11283..11936
                     /gene="nfsB"
                     /locus_tag="ACHK1J_002688"
     CDS             11283..11936
                     /gene="nfsB"
                     /locus_tag="ACHK1J_002688"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO7911933.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12194..13441
                     /gene="ybdG"
                     /locus_tag="ACHK1J_002689"
     CDS             12194..13441
                     /gene="ybdG"
                     /locus_tag="ACHK1J_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO7911934.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13509..14885)
                     /gene="pheP"
                     /locus_tag="ACHK1J_002690"
     CDS             complement(13509..14885)
                     /gene="pheP"
                     /locus_tag="ACHK1J_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO7911935.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTIDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14987..18130)
                     /gene="cusA"
                     /locus_tag="ACHK1J_002691"
     CDS             complement(14987..18130)
                     /gene="cusA"
                     /locus_tag="ACHK1J_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO7911936.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDTTLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18142..19365)
                     /gene="cusB"
                     /locus_tag="ACHK1J_002692"
     CDS             complement(18142..19365)
                     /gene="cusB"
                     /locus_tag="ACHK1J_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO7911937.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDRAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETDGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDSATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19381..19713)
                     /gene="cusF"
                     /locus_tag="ACHK1J_002693"
     CDS             complement(19381..19713)
                     /gene="cusF"
                     /locus_tag="ACHK1J_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO7911938.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19737..21119)
                     /gene="cusC"
                     /locus_tag="ACHK1J_002694"
     CDS             complement(19737..21119)
                     /gene="cusC"
                     /locus_tag="ACHK1J_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO7911939.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNGGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21276..21959
                     /gene="cusR"
                     /locus_tag="ACHK1J_002695"
     CDS             21276..21959
                     /gene="cusR"
                     /locus_tag="ACHK1J_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO7911940.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21949..23397
                     /gene="cusS"
                     /locus_tag="ACHK1J_002696"
     CDS             21949..23397
                     /gene="cusS"
                     /locus_tag="ACHK1J_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO7911941.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLVMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVDHQVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIVLPERG"
BASE COUNT         5698 a         6425 c         5980 g         5479 t
ORIGIN      
        1 tgtttagcgt tgcgcgaata atcggcccaa ttttttcaaa tgcccctgga gagataatat
       61 ccacatgcgc acaatcctga cgatagatat ccagctccgc aatccacggc gaccaggcgc
      121 gttcaggact cataccttcc tgaagcgtgc gttcagcaac aaacaacgtc gctttaccgt
      181 caaacggtac gctatgagca gtcgtcagca ggcgcacagc atcagcgtag ttgccttcaa
      241 tggtggtaaa caactccgtt gaagtacttc cctgctgtgc cgccaggaag gcctcgcgct
      301 cgcggttaat ctccgccagc acttccgggt ccagaccatt agcttctttt tcctgccagt
      361 tttgcgtttc tggcggccag gtatccagca agccaagaaa tgccacctgt tcgccacggg
      421 cacgcagccg cgccgcaata ccttgcgcca gcgtaccgcc aagtgaataa cccagcaggt
      481 aataagggcc gtgcggttgt tgttcaagta acgttgccag atgcgcttcg cagacttcat
      541 ccaggttcgt cgctgtctgc atggggccat gagggcgcgg cgactgaatg ccgataatcg
      601 accatagtgg atcgagataa cgcgagagca cgctaaactg ccaggcaaaa ccggatgccg
      661 gatggaaaca aaacagcgtc gggccattac cttcacgcaa cggcagaatg gtttcgaatc
      721 ccatgtgccg ggagctgtcc tcttcaccat caataatcgt tgccagtttg gcgacggttg
      781 acgcgaccat cacctgcccc ggcgtcacct gacgggcaaa ctgccgactt aactgcgctg
      841 ccagtttcat tgccagtagc gaatgaccac caagcgcgaa gaaatcagca tcgacatcct
      901 gcacgtcaca acccagtaac gacgagaatg ccgcggcgat aatcgtttca ctgcccgctt
      961 tcggcgcacg ccccggcgtt tgtgccttaa gttcaggcaa cggtaaggct ttgcgatcca
     1021 gcttgccgtt ggcgctaaga ggtaactgtg gaagttgcag cagaacgacc ggcaccatat
     1081 gcggcggcaa tgtttcgcga agctgtgcct gtaatgcgct ggtatccaac ggcagacctg
     1141 attgcgacac cagatagccc accaactgac gcgcatcacc accggtggct gccgcctggt
     1201 taatcacaca ggcgtgggta acggcttgtt cgacatccgg cagcgcctgc atcacgcgat
     1261 cgatttcgcc cagttcgata cgctgcccgc gaatttttag ctgatcgtca ctgcgcccga
     1321 ggtactccac cgcgccgtta tccagccagc gggcaacgtc tccggtacgg tacatccgtt
     1381 cgccaggagc aaaaggatcg gcaataaaac ggctggcggt cagatcgggt cgtccaagat
     1441 acccctgcgc cagttgaatg ccggtgagat agagatcacc cgccacaccc ggcggcaccg
     1501 gatgcatcat cgcatcaaga atacgcagac ccgtattcca taccggataa ccaatcggca
     1561 cactgctgcc gcgcacctgt gccagttcct cgccaaaagc cggataccag ctgacatcta
     1621 ccgccgcttc cgtcgggcca tatagattat gcaacggcgc gcccgttaac tgttgccatt
     1681 cgcggcataa atcagccggt aaggcctcac cactacagaa aacctgtttc aacgtcgcgc
     1741 aactctggcg agcggtttgc ggagtcagcg atgcaacaaa tgccgccagc atcgacggca
     1801 caaagtgcgt ggtcgttacg ccatattcgg caaagaattg ctgcatagcg agcgggtcgc
     1861 ggtgcgcttc cggttcagcc atcaccagtt ttgccccggc aataaacggc cagaaaaact
     1921 cccacaccga gacatcaaaa ctgcacggcg ttttttgggc aacgacatct tcgcctgtaa
     1981 gcgggtagtg attttgcatc cataacaggc ggttaacgat agccgtctgc ccgaccatta
     2041 cccctttcgg tctgccggtg gaaccagagg taaagatgat ataagccgtg tggtgcggtt
     2101 gtgaaagttg cagcggcgca ctgccctgcg gtgtaagcgg ggcgttatag caaaggttgg
     2161 ttaaatctgg aacatcggca aagcgcggca gttgatcatc ggtggtgatt aacagcgacg
     2221 gacgcgcatc ttccagcatc attttcaggc gatcgtccgg ataccccgta tccagcggta
     2281 accaggccgc acctgcttca acaatcgcat gtagcgccag ggtcaaaaag accgagcgcg
     2341 gtaatgccac cgccacactg tcccctggtt taacgccgtg ctcacgcagc agattcgcca
     2401 gcgccaccac ctgctcgcgc atttcccgat agctgaactg gtaacgcgca tctgccagcg
     2461 ccggagcatc cggtgttttt gccgcttgtt ctgccaccag cgcgctaagc gtggtttctg
     2521 gaatctcaac ctgagtggcg ttgatctgcg ccaactgcgc atactcacct ggcagcataa
     2581 tatcgacatc gccgcacaac agcgccggat ccgcggcgaa ctgggcaatc agcattttca
     2641 ggcgttcaga atgctggatt aacgttggct catcgtaacg ctgtttattg gcaaggatct
     2701 caatactcaa atcaccgtgt tcatccggga acagggccag ttcaaggtca ttaaccggcc
     2761 cggttgccag ggtatgggtt tgcgcctgaa caccaggaat gtccagttgg taatcaaata
     2821 ctttgatatt gagtaccgga ccgaacagcg gttcatcacc tgccgctcgc ccactgtcac
     2881 ggacaatttg ttcggcatcg taacgttgat ggcgacgcat ttttttcagt tgtgctgcca
     2941 gtcgggttgc cagttccggc agcgtttctt gcgccgcaat gtgaataccc aacggtaaaa
     3001 cgttgagcac gggtccggta gccgtcagcg ccgccgagcc cagtcgacgc ataaagataa
     3061 atccggcggc gtagtccatg cgattgcaca atcgccccag ccacaaggct gccagcgcaa
     3121 gggctaaatc ggtacgctgc acacctgaaa gttgcgcagc caactggcgg aattccccgt
     3181 cggtaaattc cagtttcagg cgcagaatat ctgccgaggc gctacgcccc ggtaaaggtg
     3241 ccggagaaag tgacgcgggc ggtggcagtt ggcgacgctg ttccacccag aatgccgcat
     3301 cacgctgcca ggcttcgctt tcgcggtatt gctggtactc ttccactaca tcagcgaaag
     3361 gcgtaaacgg cgaagcaggc gttggttcgc cacgcagcaa tgcgcagtaa atgttggcga
     3421 tctggcgggt aatggccgga aaactgaagc catcgaccag taaatggtga taacgctgat
     3481 accagtacca gcggttatct gccacctgta tcagctggtg aaagaccagt ggtttaccgc
     3541 tatcgacgcg caaatcttgt tgcaaatccg cctgcattaa tgcctgcgca gtaccgtgcg
     3601 gatcaatgtt ggttcgcagg tcgataattt ctggcagttc gaatatcagc gcatcatcga
     3661 cccattgcca gacttcgccg ttatcttccg taaaacgcat ccgcaacgta tctgcttgcg
     3721 ctaatccggc aaccaccgcg cgggccagta atggcgcatc aacctctccg gttaactcaa
     3781 cgtaatgcgc cacgctccag gcggagggta aatccgacag tttttctgcc atccagatgc
     3841 cgggctgtgc ggcgaccaaa ggtaaatgct ggctcattgt gcctcctgca actgggtgaa
     3901 attcgccggt gtcagagtac gccagtgggc ttcaagccac tgctggcagg actcttgtga
     3961 ctgcggctga cacacaatgt cccagcctgc cggtaaggcg cattgttgcg gccacagact
     4021 gaattgcccc tgcgcattgc gcaaaatgta aaacactccc tgtggatcat cgaagggatt
     4081 actgaatgcc atattcaact cctgtcatgg aaaagtggtt gccagaggtc gattaacccc
     4141 tgcatcaagc caccgcgcca acaaagcgca tcatgtccgc cgtcaacctg acgccagaaa
     4201 atggattctt ttatagggtg taattgcgca tacagcgcct gattggcccg catgatcatc
     4261 ggctcgcgaa tacccgcttc cagcacaatg cgcagacctt cggcgctaac ttcaccagct
     4321 tctagctttt caagtaacac gccctcttgc tgcccgcccc gatgcggcca ccagtacgat
     4381 cctgactggc ttaatacaca gccaaagcgt tcaggccagt gcagtccggc atacagcgcg
     4441 gacagcccac caaaactctg cccggcgacc acggtgcgat cggcgcgatc gctaaaaggg
     4501 gcaatagctt tcaccagggg caataactct tgctgtactg ccagccagaa atccgcatta
     4561 cacggcagtt cgtgggcgcg gtgcgtggtg tcgatagcgt cgatcaacac atacacggcg
     4621 ggaggaagtt gctgacgatg ggtcagcgaa gtcagcacag gccagacggg catactttgc
     4681 gcccaaaatt cgccatcgag caaaactgcc agcgggcgtt cttctgctgt tacatcgccg
     4741 gtggtaaaaa tccatacacg acgtgaattt ttcaaccgtt cacttttcca gataatttct
     4801 ttggctggta tttctggcgc ttgcggacaa tcccatcccg gttgcagagg cgcttgcggc
     4861 atttcgagtg cagaaacagc gtgccctcgc ccgcctttcc agctttgtgg attcagcgga
     4921 tcggctatcg cctggggtaa tagttttcgc cagccttcac gtaattcgag gcgatcgggg
     4981 gatggtgcag aaaaaatgtc atcgcgttcg gtgggaataa agcagtagct gccgcgccag
     5041 ttggcattga gttgtgtcgt ccactgccag acatcagtac ctgcaattcg ctgcatcgac
     5101 tggggctggc tgttctgatg atgatcggtc acaccagtga tgtagaccca tacacgcttt
     5161 atcgtcgagt attcttcaga accttgggga tcacgccacc agaaagtgac ctcaaacatt
     5221 tcgtcattca gacgctgcca ttccgggcca tgtttcgact gccaccagct ctcacttcct
     5281 acttttaacg ccgtcacacc ataaccccat gtttactgtg caatttttca ttgattgcag
     5341 aaatatattg ataatattat tgataactat ttgcatttgc aatagcgtaa tggcgcgccg
     5401 tgggaagcgc ggacattaat taaccaactg cactgcgtgt ctttcaggat caaaggtttt
     5461 cgcggtagcg ggatgcgtcg tgttgatgac gaccatgccc gacagttgca attcgtggca
     5521 aaaatgcagg aataaaacaa tgaacaagaa gattcattcc ctggccttgt tggtcaatct
     5581 ggggatttat ggggtagcgc aggcacaaga gccgaccgat actcctgttt cacatgacga
     5641 taccattgtc gttaccgccg ccgagcagaa cttacaggcg cctggcgttt cgaccatcac
     5701 cgcagatgaa atccgcaaaa accccgttgc ccgcgatgtg tcggagatca tccgtaccat
     5761 gccaggcgtt aacctgaccg gtaactccac cagtggtcag cgagggaata accgacagat
     5821 tgatattcgc ggtatgggtc cggaaaacac gctgattttg attgacggca agccggtaag
     5881 cagccgtaac tcggtgcgtc agggctggcg tggcgagcgc gatacccgtg gtgatacctc
     5941 ctgggtgcca cctgaaatga ttgaacgtat tgaagttctg cgtggtccgg cagctgcgcg
     6001 ttatggcaac ggcgcggcgg gcggcgtggt taacatcatt accaaaaaag gcagcggcga
     6061 gtggcacggc tcctgggacg catatttcaa tgcgccagaa cataaagagg aaggtgccac
     6121 caaacgcact aactttagcc tgaccggtcc gctgggcgac gaattcagct tccgcttgta
     6181 tggcaacctc gacaaaactc aggctgacgc gtgggatatc aaccagggcc atcagtccgc
     6241 gcgtgccgga acgtatgcca cgacgttacc agccgggcgc gaaggggtga tcaataaaga
     6301 tattaacggc gtggtgcgct gggacttcgc accattgcaa tcgctggaac tggaagcggg
     6361 ttacagccgc cagggtaacc tgtatgcggg cgacacccag aacaccaact ccgattccta
     6421 tacccgctcg aaatatggcg atgaaaccaa ccgtctgtat cgccagaact actcgctgac
     6481 ctggaacggc ggctgggata acggcgtgac caccagcaac tgggtgcagt acgaacacac
     6541 ccgtaactcg cgtattccgg aaggtctggc gggcggtacc gaagggaaat ttaacgaaaa
     6601 agcgacacag gatttcgtcg atatcgatct tgatgacgtg atgctgcaca gcgaagttaa
     6661 cctgccgatt gatttcctcg ttaaccaaac gctgacgctg ggtacggagt ggaatcagca
     6721 acggatgaag gacttaagtt ccaacaccca ggcactgacc ggaacgaata ccggtggcgc
     6781 tattgatggc gtgagtgcca ccgaccgtag cccgtattca aaagcagaaa ttttctcgct
     6841 gtttgccgaa aacaacatgg agctgactga cagcaccatc gtaacgccgg ggctgcgttt
     6901 cgatcatcac agtattgtcg gcaataactg gagcccggcg ctgaacatat cgcaaggttt
     6961 aggcgatgac ttcacgctca aaatgggcat tgcccgtgct tataaagcgc cgagcctgta
     7021 ccagactaac ccgaactaca ttctctacag taaaggtcag ggctgctatg ccagcgcggg
     7081 cggctgctat ctgcaaggta atgatgacct gaaagcggaa accagtatca acaaggagat
     7141 tggtctggag ttcaaacgtg acggctggct ggcgggcgtg acctggttcc gtaacgatta
     7201 tcgcaataag attgaagcgg gctatgtggc tgtagggcaa aacgcagccg gcaccgatct
     7261 ctatcagtgg gataacgtgc cgaaagcggt ggttgaaggt ctggaaggat cgttaaacgt
     7321 accggttagc gaaacggtga tgtggactaa taacatcact tatatgctga agagtgaaaa
     7381 caaaaccacg ggcgaccgtt tgtcgatcat cccggagtat acgttgaact caacgctgag
     7441 ctggcaggca cgagaagatt tgtcgatgca aacgaccttc acctggtacg gtaagcagca
     7501 gccgaagaag tacaactata aaggtcagcc agcggttgga ccggaaacca aagaaattag
     7561 tccttacagc attgttgggc tgagcgcgac gtgggatgtg acgaagaatg tcagtctgac
     7621 cggcggcgtg gacaatctgt tcgacaaacg tttgtggcgt gcgggtaatg cccagaccac
     7681 gggcgatttg gcaggggcca actatatcgc cggtgccggg gcgtatacct ataacgagcc
     7741 gggacgtata cctataacga gccgggacgt acgtggtata tgagcgtaaa cactcacttc
     7801 tgatgctaac gtcagattgt tgacaaagtg cgcgtcgttc atgccggatg cggcgtgaac
     7861 gccttatccg gcctacaaaa atcttgccaa ttcaatatat tgcaggacca tgtaggcctg
     7921 ataagcgtag cgcaccaggc agttttgcgt ttgtcatcag tctcgaatat ggtcgatatg
     7981 aaaactacgc atacctccct cccctttgcc gaacatacgc tgcattttgt tgagttcgat
     8041 ccggcgaatt tttgtgagca ggatttactc tggctgccgc actacgcaca actgcaacac
     8101 gctggacgta aacgtaaaac agagcattta gccggacgga tcgctgctgt ttatgccttg
     8161 cgggaatatg gctataaatg tgtgcccgca atcggcgagc tacgccaacc ggtgtggcct
     8221 gcggaggtat acggcagtat tagccactgc ggggctacgg cattggccgt ggtttcccgt
     8281 caaccgattg gcgttgatat cgaagaaatt ttttctgcgc aaacagcgac cgaattgaca
     8341 gacaacatta ttacaccagc agaacacgag cgactcgcag actgcggttt agccttttct
     8401 ctggcgctga cactggcatt ttccgccaaa gagagcgcat ttaaggcaag tgagatccaa
     8461 actgatgcag gttttctgga ctatcagata attagctgga ataaacagca ggtcatcatt
     8521 catcgtgaga atgagatgtt tgctgtgcac tggcagataa aagaaaaaat agtcataact
     8581 ctgtgccaac acgattaatt gacaacatct ggtacgattc gcccgcagcc atcactgacc
     8641 gcgggcgaaa gtgtaaagca ggtgccttac catcctgacc tgacaaccgg atatgcgggg
     8701 attactcccc gcacaacggc tacttcttcg gttcgtaagc gagaacagcc ctgaactcaa
     8761 tattacgttc cttcaccgta aactcacaca gcgagtcgcc gaccagaagc gtaaatccca
     8821 gcaccgtcaa acacagcact atgactgcca caagggcata tttcgtcagc atgtttatat
     8881 tgcctccatg cgaagaaggg actaccatcc aaattgttca ggtttggagt ggcagcccca
     8941 gggttgatag aaatatctcc tggggcttca tctttccaga cctcagagtt aacctgaaac
     9001 cggaaagctt caggcacccg ccgttatctt acccttcctc aacaaaatgg ctgtttattt
     9061 atacagtaaa tcctgtttcg gacttctcac tcctcattta atcaactgaa gctgagcccg
     9121 ttttttctgc atgcctcaat cgtcaaaatt cttcgccacc agccagccgt gccataactt
     9181 gtcacattgc aaaagtagcc aaattgcttc aggcaccatc agttcaccat caccaaaaaa
     9241 aagaaacatt ccagaacttc cgcatctcgc ccagccgctc cgacttctat actgaataga
     9301 aaacgccaac ataagagaaa cctatgccat tacccgattt tcatgtttct gaacctttta
     9361 ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc gggctatgac ttaagccagg
     9421 actcttcaat gctgattgac gcggttaaaa ataagatcac ggccggagag gtaaagcacg
     9481 atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc aaccaggctg
     9541 ccgggcaatt ttcagcgatg cagaaagtcg tattgcaggc agccgcagac catcatctgg
     9601 aaatttgcgg cggtggcacg cacccgtttc agaaatggca gcgtcaggag gtatgcgaca
     9661 acgaacgcta tcaacgaacg ctggaaaact ttggctatct catccagcag gcgaccgttt
     9721 ttggtcagca tgtccatgtt ggctgtgcca gtggcgatga cgccatttat ttgctacacg
     9781 gcttgtcacg gtttgtgccg cactttatcg ccctttccgc cgcgtcgccg tatatgcagg
     9841 gaacggatac gcgttttgcc tcctcacgac caaatatttt ttccgccttt cccgataatg
     9901 gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctgtttcgc tgcctgagtt
     9961 acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc agtcctcatt
    10021 ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac gcggtaaata
    10081 tggcgggatt aattcaggcc accgcccact ggttactgac ggaacgcccg tttaaacatc
    10141 aggaaaaaga ttacctgctg tataaattca accgtttcca ggcctgccgc tatgggctgg
    10201 aaggcgtcat taccgatccg cacaccggcg atcgtcgacc gctaacggaa gacaccttgc
    10261 gattgctgga aaaaatcgcc ccttccgcac ataaaattgg tgcatcgagc gcaattgaag
    10321 cactgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc gatttcgtcg
    10381 ccgatggcgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg gccggtgact
    10441 aagtcgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
    10501 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
    10561 tttcctctct tgcctgctgc ttcccgcccc cgcattgggg ctgacgctgg cacaaaaact
    10621 ggtgaccacg ttccatctga tggatcttag tcagctttac actttattgt tttgtctgtg
    10681 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
    10741 ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc ggcatacttt gcttattctc
    10801 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctgg
    10861 cccttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
    10921 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
    10981 tgaagtccga ttcacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
    11041 ggtatcacat gaataaaaag cactggattt cggtgtatcc cggcgaggaa atctccgaag
    11101 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
    11161 gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt tttctcgctt
    11221 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attactttca catggagtct
    11281 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca
    11341 aaaaacttac cccggaacag gccgagcaga tcaaaactct cctgcaatac agcccatcca
    11401 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
    11461 ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg taaaatactt gatgcctcgc
    11521 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
    11581 accaggaaga tgctgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
    11641 gtcgcaagtt cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
    11701 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggct ctgggtctgg
    11761 acgcggtacc catcgaaggt tttgacgccg ccatcctcga tgcagaattt ggtctgaaag
    11821 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
    11881 acgctacgct gccgaaatct cgtctgccgc aaaacatcac cttaaccgaa gtgtaattct
    11941 cctgacagcc gggaccccat cccggctcag attgctgaca aagtgcgctt tgtttatgcc
    12001 ggatgcggcg tgaccgcctt atcctgccta caagttcgtg ccaattcatt caggtaggca
    12061 tgataagcgt agcgcatcag gcaattttgt atttgccatc agccctcgca ggaaatcccc
    12121 cctcagattc tcctgatttg cataaccctg tttcagccgt catcataggc tgctgttgta
    12181 taaaggagac gttatgcagg atttaatatc ccaggttgaa gatttagcgg gtattgagat
    12241 cgatcacacc acctcgatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt
    12301 gcatattatt ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc
    12361 acggctttgg ttgcaaatca ttacccagaa taaactcttc caccgtttag cttttaccct
    12421 gcaggggatt atcgtcaata ttcaggcggt attctggctg caaaaaggca ccgaagcggc
    12481 agatattctg actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt
    12541 ctcgctgctg gatgttattt tgaatctggc gcagaaattc ccggcagcat ctcagttacc
    12601 gctgaaaggg atatttcaag ggattaaact gatcggcgcg attctggtcg gcattttgat
    12661 gatctcgctg ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc
    12721 tgccgtgctg atgttggtat ttaaagatcc gattcttggt ctggtggcag gtattcagct
    12781 ttccgcgaac gatatgctga aactgggcga ctggctggag atgccgaaat acggcgcgga
    12841 tggcgcggtg atcgatattg ggttaaccac cgtcaaagtg cgtaactggg acaataccat
    12901 taccactatt cccacctggt ctctggtttc tgactccttt aaaaactgga gcgggatgtc
    12961 agcatccggc gggcgacgta ttaagcgcag tatcagtatt gatgtcacca gtattcgttt
    13021 tcttgatgaa gacgaaatgc aacgtctgaa taaagcgcat ttgttaaagc cttatttaac
    13081 cagccgccat caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcggtatt
    13141 aaacctgcgc cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg
    13201 taaccatccg cggattcgta aagatatgac cttaatggta cgccaactgg ctccaggtga
    13261 taacggttta ccgcttgaga tctatgcgtt taccaacacc gtggtgtggc tggaatatga
    13321 aagcattcag gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg
    13381 acttcatcag tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta
    13441 attaaaaaaa ccgctctcat cgaatggatg agagcggaca tcgttttgtc ggatgtagcg
    13501 tgaatgcctt atttccgacg cagcgtttta aatgccacaa acaggaatac aatccacacc
    13561 ggcagcagga tcgctgacaa gcgcatatca tctatcgtgc acatcagcag caaaatcatg
    13621 ccgaggaagg caatgcagag atagtttccg aacggataga gcagcgcctt aaactgtgtt
    13681 tcacgcccct gacgtcgcat cgctgcacga aaacgcagat gcgccagaca gatcataatc
    13741 cagttcaaca gcagcgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc
    13801 agcagatagt tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta
    13861 atcggcacgc cgcgacggct gacgcgagtc aaaaacttcg gcgcattacc ctgtacagaa
    13921 aggccaaaca gcatgcggct gttagagtaa accccgctgt tatacactga cagcgatgct
    13981 accagaatga cgaagttcag cgcagaagct accacgttgc tgtcgagatt atggaaaatc
    14041 atcacaaacg ggctactgtt ggatttcact tccacccacg gatagagcgc cagtaaaacc
    14101 accagtgaac cgatgtaaaa cagcaggatg cgatacacca cctgatttac cgcttttgga
    14161 atgctttttt ccggatcgcg cgcttcagcg gcagtaatcc caatcagctc cagaccgccg
    14221 aaggagaaca taattaccgc cagcgacaaa atcagcccat tccagccggt ggcgaagaaa
    14281 ccaccgtagc gccagaggtt gtcgatactg gctttctcgc cgccgtgacc agaaaacagc
    14341 agccacaggc caaagccgat cataccgatg attgccagca ctttaatcag cgcaaaccag
    14401 aactcggttt cgccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag
    14461 aaggcggcag cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc
    14521 gcagcggtca gctctgccat tcccaccagc acgaacatca cccagtaatt ccagccagag
    14581 aggaagcccg cgaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc
    14641 ggctcctcaa ccaccatttc gccaagctgg cgcataatca ggaaagcgat gatcccggcg
    14701 acgccgtagc ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga
    14761 aacagaccag taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat
    14821 ccgcgatgaa gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt
    14881 ttcacgcatt tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc
    14941 atcgtcgcaa gacacaatcc acacggttaa acggggtatc ctgcttttat ttccgtaccc
    15001 gatgtcggtg cagccacatc agcttatacg ccgccgggat aataaacagc gacagcaaag
    15061 gcgcggtgat catgccgcca atcatcggcg cggcaatccg gctcatcacc tctgaccctg
    15121 ctcctgttcc ccacagaatc ggcaacagac cagcgataat caccgccacc gtcatcgctt
    15181 tcgggcgcac gcgcaggacc gcgccgtgat acaacgcctc atccagcttc tgctcgctga
    15241 atgtttgcgg attattcaac gacggctcgg cctctatggc gtgacgtaaa tacatcagca
    15301 tcaccacgcc aaattcggcg gcgaccccgg caagggcaat aaaaccggtg cccgtcgcca
    15361 cagaaagatg aaagcccatc caccacagca accagatgcc gcccaccagc gcaaacggta
    15421 cgctgctgat aatcagcaac gcttcgccca cccgacggaa tgccagatac aacagcacga
    15481 agatgatcat cagtgtcatc ggcaccatga gtttcagctt atggttggcg cgctccagta
    15541 gctcgaactg cccggagaat gccacgctgg tgcccggttt taactgcact ttttcagcta
    15601 tcgctttttg caaatcgtga accaccgaca ccatgtcacg atcgcgggca tcgatataaa
    15661 tccagctcgt cgggcgcgca ttctcggttt tcagcattga cggtccggta gagactttaa
    15721 cgtcggccac gtctgccagg gtgatttgct gcttcatcgg cgtcaggatc ggcaactggc
    15781 gcagtgcctg cggactatcg cgccagcttt gcggataacg cagattaatt ggataacggg
    15841 caatcccttc caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc acaaacaact
    15901 gcacatccgc caccgtcata ccgtaacgcg cggctttttc acggttaatc tcaacgttga
    15961 tatagcgccc accttccaga cgctcggcaa gcgcagaagc tacgcctggc accgttcgcg
    16021 ccacttcttc aatttgctcc gccatcgcgt cgatatccgc cagcacagtg ccggaaactt
    16081 taatgccgat ggggctttta atgccggttg agagcatatc gatacggtta cgaattggcg
    16141 gcacccacag attcgccagc cccggcagac gcacggtgtt atccagttcc tcaatgattt
    16201 tgtccattgt catgcctggc cgccactgat cctgcggctt aagctggatg gtcgtttcta
    16261 ccatctccag cggagcagaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc
    16321 gcgccacttc aggtacgctc ataatcagct tgtcggtttt ttgcagcata ctcgccgcct
    16381 ctgctgcgga aatccccggc agcgtcgatg gcatatacaa caagtcgcct tcattgatct
    16441 gcggtaaaaa ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg
    16501 ctgccaccaa cagcgtggtt ttcggccagt gcagtacttt cagcaataga ggatgataaa
    16561 cacgaatcaa aaagcgattg agcgggttac tgctttccgg cggaattttg ccacggatcc
    16621 agtaacccat caggataggg atcactacga tcgccagcag cgccgcaccc gccatcgcat
    16681 acgttttggt gaacgccagc gggccaaaca gacgcccttc ctgcccttcc agggtgaaga
    16741 tcgggataaa cgacagtgtg ataatcagca gactgataaa cagcgccggt cccacttcaa
    16801 cagacgcatc ggtaatcacc tgccagcgcg ttttattatc cagcgtggtg tcaggatgct
    16861 ggtgctgcca ctcttccagc cgtttatgcg cattctcgat catgacgata gcggcatcga
    16921 ccatcgcccc gacggcaatc gcaataccgc ccagcgacat aatattggca ttcagcccct
    16981 ggaagtgcat gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca
    17041 gcgccgagcg cacatgccag agaaacagcg cacagactac cgccaccaca ataaactctt
    17101 ccagcaactt gccgctgaga ttgtcgatgg cccggtcaat gagctggctg cgatcgtatg
    17161 ttgtaactat ctccacgcct tccggcagac tgcttttcag cgtttctagt ttgtccttca
    17221 cggcggcgat cacttcacgg gcgtttttgc cggagcgtag gatcaccacc ccgcccgcaa
    17281 cttcgccttc gccgttcagt tcggcaatgc cccggcgcat ctccgggcca acctggatct
    17341 tcgcaacatc acgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat
    17401 taaagtcgtc gagcgtttgt agatagccgc tggcgcgcac catatattct gcttccgcca
    17461 gttcgattga cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt ttaacttcgg
    17521 cgagactgat gccatactgc gccaggcgct ggggatcgat aaccacctga tactctttca
    17581 ccacgccgcc caccgacgcc acttccgcaa cgtcagggat ggtttttagc tcatatttga
    17641 gaaaccagtc ctgcaatgag cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca
    17701 gtgcatattc atagatccag ccaacacccg tggcatctgg ccccagctcg gcgctgactc
    17761 ccgcaggcag cttaccctgt acctggttga ggtactccag cacccgcgag cgcgcccagt
    17821 acggatcggt gccatcttcg aaaatgacat acacataaga gtcgccaaac tgcgagaaac
    17881 cgcgcacagt cttcgcgcca ggtaccgaca acatggtggt agttagcgga taagtcacct
    17941 gattttcaac gatttgcggt gcctgcccgg gatagctggt tttaataatc acctgcacat
    18001 cggagagatc cggcaacgca tccactggcg tgttaatgat ggtccaggtg ccccagatac
    18061 tcagaaataa cgcgcccatc agcaccagaa aacggttcgc caccgagcga cgaataatcc
    18121 attcaatcat tggttattcc ctcaatgcgc atgggtagca ctttcagagc gcatccgctc
    18181 cagtgcgcca gaaatattgg cttcggaatc aatcaggaac aggccgctgg aaaccacctt
    18241 ttcaccttcc gccagaccag agcgtaacgc ggtgacacct tgcgatgcct ggaaaacggc
    18301 aacgcgtttc ggtacaaagc gcccgtcggc atcaacggta atcacccgct gttcgttgcc
    18361 ggtatcaatc agcgcttgtg acggaatgag cagcatcggt tcgctggcgg tattgagttg
    18421 caaccaggcg ttcattcccg gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag
    18481 ctgcagcgtg cgggtcgcgg aatccacgcc aggtagtagc gtccatttgc ggatggtgag
    18541 tgttttatca ggtcgcgccg gaacggtgag cgtaaactgc gaggcatctt tcaccagcca
    18601 ggcgatggac tccgggatcg cagcagtgac ccacaccggg tccataccct gaattttcgc
    18661 gaccacgtta tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc
    18721 atcaatgggt gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg
    18781 aatatccgcc tccggcattc ccgccagtcg cagccgctca agaatgcctt cagtctgggt
    18841 cgccgtaccg tcggtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcggg
    18901 aatggtcagg tcgagaagcg gtgtgccctt ttgcacttta tcgcccacgg taagcggata
    18961 caccttgtcg ataaacccgg cagcgcgagc ctgcacaatg gcatactgat actcgttgta
    19021 actgacattc gccgggaaac tctgggcaaa agtcagcggt ccgcgcgtga cggtagccgt
    19081 tttcaccccc agattctgcg tctgagtcgg gtcaatgcgc acaccagacg cagaactctc
    19141 ttcatcggca tatttcggca ccagatccat atccataaac ggcgatttac ctggtttatc
    19201 gaaccgcgta ttgggataca tcgggtcgta ccagaataag attttacgtt ctgcagtcga
    19261 cgttttttct gcgggcggtt cagcccggtc aaaccaggta aaacctgccg cagaaataat
    19321 accgcccgcg atcatgctgc cgataataag cgcgattttt ttcatctcat taaacctggg
    19381 ttactggctg actttaatat cctgtaataa agaaaggttg ccctgctgga caaaattaaa
    19441 cgccactttg tcgccagttt taatttcact cattttcgtc tgcggagtga tggtaaagcg
    19501 catagtcatc tccggccagt tcacggcagc aatcggatcg tgatggatgg tgattttttt
    19561 gctttccaga tcaacacctt ttaccacgcc ggtggcacta atgacctgtg gttgtgcttc
    19621 gctcatggtt tcatgatgat gttcgttagc ctgggcatta aagccaataa cggtaaacag
    19681 actgaacatt gcgacttgca gtgctttttt catttactct ctcctggagt taaaaattat
    19741 tgctgccaac cgccaccaag tgcggtatat aaagaaattt cgttgacctg acgggcataa
    19801 ttcaggtcaa gtaaggtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc
    19861 agataactta ccgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc
    19921 gacgccagat aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc
    19981 gcatctgcca cttctttaaa ggcgttctgg attttctgtt cataattcac taccgactgc
    20041 tgctggcgaa tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg
    20101 atctcaattt tgggtataaa attccacatc ccgctgctgg cgttaaataa tgacgacaga
    20161 tcgctgctgg cggttgatat tccgctggtc aggctgatag acgggaaaaa tgccgcgcgt
    20221 gcagcaccaa tattggcatt agccgccatt aacgcatgtt cagcttccat aatatccggg
    20281 cgctgcaata agatttgcga cgacaagccc gccggtaatt taacgctttg cagactgccg
    20341 ccgtttactg tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca
    20401 ttattcgcct gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttcaatc
    20461 accccgcgag cctgttccag cgccagaaca ttactgctac ctgtcaacag ttgtttttcg
    20521 acaaacgcat atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca
    20581 tacgccagct gctgattgaa atagctttgc gcgacattag aaaccagcag aatatgcacc
    20641 gcgcgctgag cttcctcagt ggctaaataa ttttgtcgct cggcttcgct catgttcttt
    20701 aagcgcccga aaaaatcgag gtcaaagctg gcgttaaggc cagtagaaaa ctcccgcgtc
    20761 gtggctgaat cgcctttaag attgccgctc cagctgccgc tgccctcacc attgagctgt
    20821 gggtagcggt cggcatcagt cagacgatat tgcgcccgtg cctcctgcac tttcagcgtc
    20881 gccatgcgca aatcccggtt attctccagc gcctcgctaa tcagcgtctt cacctgatta
    20941 tcaacaaaaa aggtgcgcca gcccgcgttc tgatagttat ctgctgcgtt aaccaggccg
    21001 ttctggctga gtgagaactg ctgaggaacg ggcattgccg gacgctgata atccggtgcc
    21061 agtgaacaac cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata
    21121 ggctcataat ttctggtgat tttataccgc caactttact cgccaggctc tgattttccg
    21181 gtgacaggaa aatgacaaaa ttgtcatttt gccaataagc gattgccatc tgatcccgct
    21241 actctagaat tgcccgggca acatgcggag gaaatatgaa actgttgatt gtcgaagatg
    21301 aaaagaaaac cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt
    21361 tggccgacaa cgggctgaat ggctaccatc tggcgatgac cggtgattat gatctgataa
    21421 tcctcgatat tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttacgctccg
    21481 ccaataaagg gatgccgatt ctgttgctta ccgcgcttgg caccattgaa catcgcgtca
    21541 aggggctgga gttgggggca gatgactacc tggtgaaacc attcgctttt gctgaactac
    21601 tggcgcgggt gcgcacatta ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc
    21661 aggttgccga tttgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca
    21721 tcactttgac cagtaaagag tttactctgc tggagttttt tctccgccat cagggcgaag
    21781 tgctgccccg ctcgcttatc gcctcgcagg tatgggacat gaattttgac agcgacacta
    21841 acgccattga tgtggcggtg aagcggctgc gcggcaaaat cgacaacgac tttgagccga
    21901 agctaattca gaccgtgcgc ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag
    21961 ccatttcagc gcccgttttc gctggcaacc cgcctgacct tttttatcag cctggccacc
    22021 atcgcggcgt ttttcgcctt tgcatggatc atgatccact cagtaaaagt gcattttgcc
    22081 gagcaggata ttaatgattt aaaagagatt agcgccaccc ttgaacgcgt actaaatcac
    22141 cctgacgaaa cgcaagcccg acgcttaatg acgctggaag atatcgtcag tggttattcc
    22201 aacgtgttga tttccctggc agatagccac ggtaaaacgg tgtatcactc ccccggtgcg
    22261 ccggatatcc gcgagtttac gcgtgacgcc atacccgata aagacgctca gggtggcgag
    22321 gtgtatctcc tttccggccc gacgatgatg atgccaggcc acggtcacgg gcatatggaa
    22381 cacagcaact ggcggatgat cagtttgcca gttggcccac tggttgacgg caaaccgatt
    22441 tatacgctct acatcgcgct ttcgatcgat tttcatcttc attacataaa tgatttgatg
    22501 aataaacttg taatgaccgc atcgatcatc agcatcctga ttgtctttat cgtgctattg
    22561 gcggtacata aaggtcacgc gccgattcgc agcgtcagcc gtcaaatcca gaatattacc
    22621 tcgaaagatc tcgacgttcg cctcgacccg cagaccgtgc cgattgagct ggaacagctg
    22681 gtactgtcgt tcaaccatat gatcgagcgt attgaggatg tctttacccg ccagtccaat
    22741 ttctcagcgg atatcgctca cgaaattcgt acgccgatta ccaatctcat cacgcaaacg
    22801 gaaatcgccc tcagccagtc tcgcagccag aaggagctgg aagatgtgct ctactctaat
    22861 ctcgaagagc tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct cgcccaggcc
    22921 gataacaacc agctaatccc cgaaaagaaa atgctcaacc tggcggatga aatcggcaaa
    22981 gtgttcgatt ttttcgaggc gttagcggaa gatcgcggcg tggagttgcg gtttgttggc
    23041 gacaaatgtc aggttgcggg cgatccgctg atgctgcgtc gggcgttaag caacctgctt
    23101 tctaatgccc tgcgttatac gccacccagt gaggctattg tagtgcgctg ccagacggtc
    23161 gatcaccagg tgcaagttat cgtcgaaaac cccggtactc ccattgcgcc cgagcactta
    23221 ccgcgattgt ttgaccgttt ctaccgcgtt gacccttccc gccagcgaaa aggcgaaggt
    23281 agcggcattg ggctggcgat agtgaaatcg attgttgtcg cacataaagg cacggttgca
    23341 gtaacgtcag atgcgcgggg gacaaggttt gtgattgtgt tgccggagag ggggtgatta
    23401 ttaagttcaa attcagggcg agggtttaca gcgtttttcg ctaaatttcc atcattataa
    23461 acaagccacc tatcatataa agcaatgaat aattaaatcg cgattaatca tcattaattt
    23521 ttatattatt cacccaatcc atttataaag tgaatttgat aaattcctaa tttaaaatat
    23581 cc
//
LOCUS       DAZGBC010000072        23402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGBC010000072 DAZGBC010000000
VERSION     DAZGBC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.072"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..270)
                     /gene="chbB"
                     /locus_tag="ACHK1J_002697"
     CDS             complement(<1..270)
                     /gene="chbB"
                     /locus_tag="ACHK1J_002697"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO7911942.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGL"
     gene            complement(569..907)
                     /gene="osmE"
                     /locus_tag="ACHK1J_002698"
     CDS             complement(569..907)
                     /gene="osmE"
                     /locus_tag="ACHK1J_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO7911943.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1109..1936
                     /gene="nadE"
                     /locus_tag="ACHK1J_002699"
     CDS             1109..1936
                     /gene="nadE"
                     /locus_tag="ACHK1J_002699"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO7911944.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2166..3053
                     /gene="cho"
                     /locus_tag="ACHK1J_002700"
     CDS             2166..3053
                     /gene="cho"
                     /locus_tag="ACHK1J_002700"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO7911945.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSQAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3013..3588)
                     /gene="ves"
                     /locus_tag="ACHK1J_002701"
     CDS             complement(3013..3588)
                     /gene="ves"
                     /locus_tag="ACHK1J_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO7911946.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3791..4276)
                     /gene="spy"
                     /locus_tag="ACHK1J_002702"
     CDS             complement(3791..4276)
                     /gene="spy"
                     /locus_tag="ACHK1J_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO7911947.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4606..5574)
                     /gene="astE"
                     /locus_tag="ACHK1J_002703"
     CDS             complement(4606..5574)
                     /gene="astE"
                     /locus_tag="ACHK1J_002703"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO7911948.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5567..6910)
                     /gene="astB"
                     /locus_tag="ACHK1J_002704"
     CDS             complement(5567..6910)
                     /gene="astB"
                     /locus_tag="ACHK1J_002704"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO7911949.1"
                     /translation="MNAWEVNFDGLVGLTHNYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREKGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6907..8385)
                     /gene="astD"
                     /locus_tag="ACHK1J_002705"
     CDS             complement(6907..8385)
                     /gene="astD"
                     /locus_tag="ACHK1J_002705"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO7911950.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAVFPRWARLSFAERQAVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPHLLQAGTSLLTPGIIEMTCVAGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFEQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8382..9416)
                     /gene="astA"
                     /locus_tag="ACHK1J_002706"
     CDS             complement(8382..9416)
                     /gene="astA"
                     /locus_tag="ACHK1J_002706"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO7911951.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGIVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9413..10633)
                     /gene="astC"
                     /locus_tag="ACHK1J_002707"
     CDS             complement(9413..10633)
                     /gene="astC"
                     /locus_tag="ACHK1J_002707"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO7911952.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACERFVSGGSS"
     gene            11079..11885
                     /gene="xthA"
                     /locus_tag="ACHK1J_002708"
     CDS             11079..11885
                     /gene="xthA"
                     /locus_tag="ACHK1J_002708"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO7911953.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12052..12762
                     /gene="ydjX"
                     /locus_tag="ACHK1J_002709"
     CDS             12052..12762
                     /gene="ydjX"
                     /locus_tag="ACHK1J_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7911954.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFKAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTAIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            12767..13444
                     /gene="ydjY"
                     /locus_tag="ACHK1J_002710"
     CDS             12767..13444
                     /gene="ydjY"
                     /locus_tag="ACHK1J_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO7911955.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VIFKITE"
     gene            13459..14166
                     /gene="ydjZ"
                     /locus_tag="ACHK1J_002711"
     CDS             13459..14166
                     /gene="ydjZ"
                     /locus_tag="ACHK1J_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7911956.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14166..14714
                     /gene="ynjA"
                     /locus_tag="ACHK1J_002712"
     CDS             14166..14714
                     /gene="ynjA"
                     /locus_tag="ACHK1J_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO7911957.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14724..15890
                     /gene="ynjB"
                     /locus_tag="ACHK1J_002713"
     CDS             14724..15890
                     /gene="ynjB"
                     /locus_tag="ACHK1J_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7911958.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVQEDFSVPTEGAESPWGGAQLTFIARRD
                     VPPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTADPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKSGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15863..17398
                     /gene="ynjC"
                     /locus_tag="ACHK1J_002714"
     CDS             15863..17398
                     /gene="ynjC"
                     /locus_tag="ACHK1J_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO7911959.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLMIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVILLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDRSWTAVVWGHLLWVIPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17398..18051
                     /gene="ynjD"
                     /locus_tag="ACHK1J_002715"
     CDS             17398..18051
                     /gene="ynjD"
                     /locus_tag="ACHK1J_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7911960.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALPGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDFQDVPADSSVLDMAQWSENYNKLR"
     gene            18118..19425
                     /gene="ynjE"
                     /locus_tag="ACHK1J_002716"
     CDS             18118..19425
                     /gene="ynjE"
                     /locus_tag="ACHK1J_002716"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO7911961.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVNTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TEKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIRPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19434..20054)
                     /gene="ynjF"
                     /locus_tag="ACHK1J_002717"
     CDS             complement(19434..20054)
                     /gene="ynjF"
                     /locus_tag="ACHK1J_002717"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7911962.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPTCFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20141..20548
                     /gene="nudG"
                     /locus_tag="ACHK1J_002718"
     CDS             20141..20548
                     /gene="nudG"
                     /locus_tag="ACHK1J_002718"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO7911963.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20514..20786)
                     /gene="ynjH"
                     /locus_tag="ACHK1J_002719"
     CDS             complement(20514..20786)
                     /gene="ynjH"
                     /locus_tag="ACHK1J_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO7911964.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLNTNPLVWQRVKP"
     gene            21022..22365
                     /gene="gdhA"
                     /locus_tag="ACHK1J_002720"
     CDS             21022..22365
                     /gene="gdhA"
                     /locus_tag="ACHK1J_002720"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO7911965.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGKQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22482..>23402)
                     /locus_tag="ACHK1J_002721"
     CDS             complement(22482..>23402)
                     /locus_tag="ACHK1J_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7911966.1"
                     /translation="KWGFYLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLITMG
                     LIVRAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPE
                     PGKDTFQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKAN
                     MEEAPEQVIALLNKFAVFPSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNG
                     TIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYR
                     YDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         5624 a         6180 c         5944 g         5654 t
ORIGIN      
        1 taaaccatcg actttgccat aaagcagcga gtcaattact tcaaccggtt tgttgggtaa
       61 caaacgctgg atttcgggca acatataagc aatctgcggc cctaataaca cgacatcggc
      121 attctgacct ttttcaccag ccagtgtttc cggaaatgct tcaataatga ccggaacttc
      181 atatttttct gcctgtgcgc gcatttttga taccagtaaa gaggtagaca tgcccgcaga
      241 acaaaacaga taaatgtgtt tcttttccat aaaactgccc tcatcgacga ttatctgtca
      301 gccagacact ccgcaagcct taacctgctt ccatgctctg ggtaacttgc gaaaccaaca
      361 tgactttttt tgtggctgaa aggagtatac ggtatagacc tgagcaatag tatttctttg
      421 accttataaa ttgtctctcc ttgacctgcg gttatgaccc tcttcacatt tcgggcaaat
      481 aattcgcgaa gataattaag aactcagacc aaaaaaaacc ggcgcaatgg ccggtttccg
      541 ttgttactca agctttcaga cgaagtgatt acttcgcagc ctgtggatca gtgtcgtatt
      601 cagcacaggt ctgataaccg gagttgatga catgtccggt atcatctaac gcgacaaagt
      661 aggtttctgc tttaccatca cgttgaccca ggatgtaggt ctggcaagta ccacgagcat
      721 ggatcatgct cacttcagac gaaggtttac ccgcaatttg tgcaacctgc gcccggctca
      781 tgcctttttt gacgtctttc accacaggct gtacaaactg gtctttggta cgatcataag
      841 ccgtacaacc cgccagcatg gttaataccg ccgctgcact cagaattcct gccatattct
      901 tgttcatatt ccgtcctctt gtttatcagc gtgttagata agcctggaat acattgggcg
      961 ctttttcaag cccgtgaacg aaacggctcc gctttcagag gattcctgta tgacgtttta
     1021 accaccattc agcccgctgt cgcttgtcgt ttcagtagca acgggttagc tttaaggaag
     1081 ttttgtcttt tctgtctgga ggggttcaat gacattgcaa caacaaataa taaaggcgct
     1141 gggcgcaaaa ccgcagatta atgccgaaga ggaaattcgt cgtagtgtcg attttctgaa
     1201 aagctacctg cgaacttatc cgttcattaa atcactggtg ctcgggatca gcggcggtca
     1261 ggactccacg cttgccggta agctgtgcca gatggcgatt aatgagctgc gccaggaaac
     1321 cggcaacgaa tcactgcaat ttattgccgt acgcctgccc tatggtgttc aggccgacga
     1381 acaagattgc caggatgcca ttgcctttat tcaaccggat cgcgtattaa ccgttaatat
     1441 caagggcgcg gtattggcta gcgagcaggc attgcgggaa gcaggcattg aactgagcga
     1501 ttttgtccgt ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata gcattgcggg
     1561 tatgaccagc ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta ccggattctt
     1621 cactaaatat ggtgacggcg gtacagacat taatccgctg tatcgtctca acaaacgtca
     1681 gggtaaacag ttactggcgg cattaggttg cccggaacac ctttataaga aagcgccaac
     1741 ggccgatctg gaagatgatc gcccttctct gccagatgaa gtggcactcg gcgtgaccta
     1801 tgacaatatc gacgactatc tggaagggaa aaacgtacct caacaggtcg ccagaacaat
     1861 agagaactgg tatctgaaaa ccgaacataa acgccgtccg ccaattaccg ttttcgatga
     1921 tttctggaaa aagtaataat ttgcacctat tggattgtgc gaaaaagagt aatttgttca
     1981 cgccggatgc ggcgtgaacg ccttattcga cctacaaaac tatgcaaatt caatacattg
     2041 caggagtcga atgggcctga caggcgtagc acgtcagacg gtgtaacctt tgtcatcaac
     2101 ccgcctcttt tttaatcgct tcacgcctgt tacactggat agataaccag ccttcggagt
     2161 caacagtggt acggcgttta acttctccgc ggctcgaatt tgaagctgcg gcaatttatg
     2221 aatatcccga acatttacgt tcattcctta atgacttacc cacccgacca ggggtgtatc
     2281 tgtttcatgg cgaaagtgac accatgccgc tctatatcgg caaaagcgtt aatatccgca
     2341 gtcgggtgct ttctcattta cgcaccccgg atgaagccgc catgctacgg caatcccggc
     2401 gaattagctg gatctgcacc gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa
     2461 tcaaagaaca acagccgctg tttaataaac ggttgcgccg caatcgccag ctctgtgccc
     2521 tgcaattaaa tgaaaagcgc gtcgatgtgg tgtatgccaa agaggtggat ttttcacaag
     2581 cccccaacct gtttggcctg tttgccaata ggcgcgcagc tttgcaagca ttgcagacca
     2641 tcgctgatga acaaaaactt tgttatggcc tgctgggact ggaaccgtta agtcgcggtc
     2701 gtgcatgttt tcgttcagcg ctaaaacgtt gcgccggagc atgctgcggt aaagagagcc
     2761 atgaggaaca tgcgctacgc ttgcgccagt ctctggagcg tttgcgggtg gtgtgttggc
     2821 cttggcaagg tgcggtggca ctgaaagaac agcacccgga aatgactcaa tatcatatta
     2881 ttcaaaactg gctgtggctg ggggcggtta attcgcttga agaagcgaca acgttaattc
     2941 ggacacccgc cgggtttgat cacgacggtt ataaaattct ttgtaagccg ctgctttccg
     3001 gtaactatga aattactgaa cttgatccgg cgaatgacca gcgagccagt tgatttcact
     3061 gaacaacagt ttaccttgcg gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca
     3121 cgcgccctgg tcggtagtga gcagtttgtc gcccagttgc cacgccccgt taatcacaaa
     3181 caccaccccg ccgcgtgagc caaaggtagt aaacgtacgt tcggcaattc tgactttcgc
     3241 cttacagaca tccaggcgtg tcatgatgtt gaaatccatc gacatctgcc cttccgtcag
     3301 tttcgctttt accacctggt ccgccgcaaa ggcaaaaggt tgtaacggtt ttaaagtatg
     3361 gttaaagcgg tctgtgcttt caagaaacat ctcgccgcct tccagcaacg tcactatcct
     3421 ttccataccg ggaaacaaag aaaactcccc attagccgcg atggatgcaa tgctggcacg
     3481 ccagtaaaaa tcacgttttg ccggtgggaa agtgcaaatt tcgcgcgttt caccggcagc
     3541 gtttcgccac agattcaccg acattttacg catatcaaag tattccatac tcgctcccgg
     3601 cccgttcttt tcattgttat cgttcaggta accgtggatg gttatcggca actcgggtga
     3661 tttacgttag tggtgatcag gcttttttta caactgtttg agaagagaaa agaaaaccgc
     3721 cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt
     3781 ggctgaaaga ttattcagca gttgcaggca ttttaccttt tgccgctgga cgttctgtca
     3841 gacgcttctc aaaattagca ttaaattgct ttttctgttc cggcgtcagg atgttgtaaa
     3901 ttttgttctg ggtttccatg tgcgccagca tgttagcttt gcgctgttct tccatttttg
     3961 cgatctgcgc ttcagctttt gctttatcga aggtatcgct ggcaatgatg tcatgcattg
     4021 cgcggcgttc ttccagcggc ggacgtttca tctggtcacg ctggcctttc atgatttcgc
     4081 ggatctgctg tttctgcgcg tcggtcaggt tcaggtcttt gaacatcatg tcctgatgcg
     4141 gaccgaactt gcctttgtgg tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg
     4201 tgtctgcggc atgggccagg ttagccgcgc caagagccag ggtagaggca acaaacagtg
     4261 cagttaattt acgcatattc tatatccttc ctttcagtta tttattacgg ctttcttaag
     4321 tagcgtgccg tgttgacgag attaacttta ctgactttag cgtcaattaa tcagagcaac
     4381 ggtaaaacaa tgaaagtgta aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat
     4441 ttgatggaga gtgatgagcg aatattacaa cacgatgatt ttgcagagat tatgaagaac
     4501 tataccggat gactggtgat aaataaagca aataaccagg attaatctgt attaatttat
     4561 aagaaagcaa cttaataccc gcagaatgat ttctgcgggt aagtattagc ttattttttc
     4621 gagcattaat cccgcgcgta accccaacgc taccaacgga ttagggaata acacatactc
     4681 tacatcatgg gttacggtaa aacgttcctc accgtcctgc gccagcaacg ttcctttctt
     4741 aaacggcata aaattcagcg tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg
     4801 agtaatttgc gaaaccaccc gataacggag cggcggtgtt ctcacgatac cgacactctc
     4861 accagatagc agcgcagcaa ttgcgctggc agttactgca aactggcgaa gatcgttttg
     4921 cccaaagggc aacgctttgc caagttccag cgtacaggcc agcgcgccaa aatgtctggc
     4981 gctgaaatgg gtaaacgtac caccaggctc ctgatggaac accagcgcct ccagccccgc
     5041 cgcacccagc cacgtcagaa atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc
     5101 gaactgcgga tgcaaggagc cacgaattgc ggtatgtaga tcaaggtgcc agcgcacaga
     5161 ttctttgccc tggtcataaa aatcttccag gcactgttcc agttcgcgcg cccgacaggt
     5221 ttctccgctt tcagcaaaca gctgccaacg accgccaaac attcggttca tatcgctatg
     5281 gcaataacgt ttcccttgct tcagcgcagg aggattcccg aggatcacca gcaaccgcca
     5341 acgtaacggg atctcgccgt gagatatcgc gccaagcaac gcgtccagca tttccaccgg
     5401 agccgtttca ttaccgtgga tccccgctga aatcaccagt gcgccttgcg gtggcgttaa
     5461 tggcgtcagt tccagcacac catcgcccag ccagcgccag cgaacaccgt tgatttctcg
     5521 ctcggtgata accggttttt tactcgttaa ggtcagagca agaaaattat ccattgcccc
     5581 ctccctcgcg ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca
     5641 gtgcttcccg cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaagacgat
     5701 cgcggtagta acgatccacc cagtcattga gcgcattaaa caaggtatcg ttcatcatca
     5761 ccgccggatt caccgcccgg cgttcttctt gtgtcaatac cacccgcaac cgcaggcacg
     5821 ccgggccacc gccattcgcc atgctttcac gtaaatcaaa gacttttagt tcgctgatcg
     5881 ggttgtcagc ggcaaggagt tcattgagat aacaccatac tccggcgtgt tcccgacact
     5941 cctgtggcag caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca
     6001 gatatgtaga caccgcatcc gacacggaaa cctgagttgc cggaacttct atcgccataa
     6061 aaccgttcac ccgcgcacgc aggtttgcca gtaactgtga ctggcgagcg aacgcctgtt
     6121 ggtggcaaaa cagcacctgg cggttactca cggcaatcac gtcattatga aaaacgccct
     6181 ggtcgataac gtccgggttt tgctgggcga aaatcacctg ttggggattc acctgattca
     6241 gccttgccac agcctcgctg gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg
     6301 tatcattgcc tttttctcgc ccgtagacaa aaagttgcat acccggttca ccgtaatgac
     6361 cgccgagacg attgtggttt gccgccccct catcaccgag caacgctacc tgcggcaacg
     6421 ccgaatggac gctaaatttc tcttcgtcgt taaaaatagc ttttaacagc gattcagtga
     6481 cgggcgcttc cagcgaacgg tgaaatttat tgttcaggtt ggcaacggtg agatgcactt
     6541 tgccatccag cgtatcggca gatggcgcga tcgttgccgc attggctacc cacattggcg
     6601 aagcggagct gacgctggaa agccagtgcg gtgcctggcg tgcaactttt tccagtacct
     6661 gctcatcgct accactgaat cccaactgac gcagcaccgg aataaacgga cgctcgtgcg
     6721 gcgggatcac ggcctggggg aatcccgcat cggcaagggt tttcattttc agtaagccct
     6781 gtttcgccgc cagtcgcggg ttagacacct gaaaacggtg acgggtagag gcttcattac
     6841 caaacgacag gccagcgtaa ttgtgtgtca gccctaccag cccgtcgaaa ttaacttccc
     6901 aagcgttcat cgcaccacct catcggaaaa atccagtccg gggttaagcg tggcgggtaa
     6961 cgttaacgag tccgactcca ggctcgccat aggccatgcg cagtaatctg cggcatacca
     7021 ggcgctgggg cgatggttac cggatgcacc aatgccgccg aatggcgcgg tactggcagc
     7081 accagtaagc ggtttgttcc agttaacaat ccccgcccgc gcttccagca acagttgttc
     7141 gaacttttcc cgctcggggg aaaccagacc gcaagagagg ccgaagcgag tgttattcgc
     7201 catcctgatc gcctcatcga aattgtcata acgccagacg cgcaagagtg gcccaaacac
     7261 ttcttcatcc ggcacgccag caacgcacgt catttcaatg atccccggcg tcagcaacga
     7321 tgttccggct tgcagcaagt gcggcgcaag cagggttcgt ccgcccatcg cttccagttg
     7381 ctgccaggca gtaaccacct gctgtgcggc ctgttcagaa atcagcccgc caataaacgg
     7441 ctgcggttcg tcatcccagt tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc
     7501 aagaaacgca tcgccctgcg ccccgctttt cagcaataaa cggcgggcgc aggtgcagcg
     7561 ttgaccggct gtgacaaacg ccgactgaat ggtcagatgg acagcggcgt cgatatccgc
     7621 cacctcatcg ataattagcg gattattacc gcccatctca agggcgagaa ttttctccgg
     7681 ctgaccggag agctggcgat gcagctggta gccggtattg gcgctaccgg taaacagcaa
     7741 gccgtcgaga tcctccagcg cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag
     7801 gttcagcacg cccggcggca agccagcctg ctgccataaa cgcattaccg cttcgccact
     7861 ccacggtgtc agttcgctgg gtttaaagat aatggtgtta cctgccagca atgccggaac
     7921 gatatgtccg ttcggcaaat gaccagggaa attatacggc ccaaacaccg ccagcacgcc
     7981 gtgcgggcga tgtcgcaggc tcgccgcgcc gtccggcatt tcactacgct gctcgccggt
     8041 acgaacgtga tacgccttaa ttgaaatcgc gattttattg atcatcgccg tcacttcggt
     8101 tgccgcttcc cagcgcggct tgccggtttc tcgggcaatg attgcggtta attcggcttt
     8161 attgctttcc agcaatccgg caaagcgttc gacaactgcc tgacgttcag caaatgagag
     8221 ccgcgcccag cgcggaaaca ccgcacgggc tgcgcgacaa gcctgcccga cctgagcggc
     8281 atcggcatca ttgccttgcc ataacacctc gcccgatacc ggattacgct tcacacgcga
     8341 tgcaccctgg cccgttatcc agtcaccgtt aatccataaa gtcatgctgt tttctcctct
     8401 gcgcacaggc gcaccagacg aacgcgatcc cctgcgtggc atttgagggc atccagttgt
     8461 gcggcggtta aaatcaaacg ctcggttgcc ggatcggtac gcaccagcac cacgcggaaa
     8521 tggtgataat tttcattggc gaccaggcag gccggaaaat cgccctgcgc aggctgccct
     8581 tctgccactt ccaccagccg acttttacgg atggcgcgca cgcggtcgat gtcacactca
     8641 agcgtcggcc caccgtcgaa gatgtcgata taattacggt agcgaaaacc ttctttctcc
     8701 agcaccgcgc gggcaggcgc ggtttgcgga tgtacctgac cgatgacgtc ctgggcctcc
     8761 tgggataaaa agtgcgtata gatcggatgt ttcggcatca gttctgcaat aaatgccttt
     8821 tgcccggtgc cgcagagaaa atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg
     8881 aggctttgcc agaacggtga atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc
     8941 actttgtcat taaacttgtc gcgaaaagcc gccataaaca taaagcgcga tttcgacagc
     9001 aaatagccgt tgccctcttt gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg
     9061 ctgccggtgt gatcgttact gagaaacagc gttggcagcg cattatagac attcagctct
     9121 ttcgaagcgt gaaccaacgt gccgacgcga tagttgtacc agggatcgtt cagcccaact
     9181 gccacctcaa tggcgcaaat ccccgccaca atgcctgtct cgctatcttc cagtacaaac
     9241 acataaccct gctcactttt gggcagttca ccttgccagg ttttgattgc cctttcgata
     9301 cgcgccgaaa gcgtggcttc attggcggga agcgacgtca ggccgccgcc cgttttgctg
     9361 gcaagctgca tcagcgccga gacatctgag cgttcaacgg gacggatgac catcatgatg
     9421 aacctccgct cacaaagcgt tcacaggccg cagcaaagcg atccagtccg gtcgtcacct
     9481 cttcttcgct gacattgagc gcaggcgcaa aacgcaccac gttgccaccc gcaatcagca
     9541 ccatcacgcc cgctttcgcc gcctcctgag agatctgttt cgcttgtccg gcgtaatcgg
     9601 cattcagtac acagccaatc agcaaaccta agccgcgaac ttcactgaac aaaccatagc
     9661 ggtgattaat aatattaaga cgctcaacaa accagtcgtg acgttgctta accccgttaa
     9721 gcatctctgg cgtattgatg agctccagca ctttgcctgc caccgccgag gccagcggat
     9781 tgccgccata ggtggtgcca tgagtgccaa cggtcatcac gctggcgcac tcttcggttg
     9841 ctaacaacgc accgaccggg aaaccgccgc ccagcgcttt ggcggtagtt aacagatcag
     9901 gcgtcacgcc gtagtgcata taggcataca gttccccggt gcgcccgacg ccggtttgta
     9961 cttcatcaaa aatcaacagc gcattgtggc gatcacacaa ttcacgcaga ccttgtaaaa
    10021 atgcgttgct ggctggcacc acaccgcctt cgccctggat gggttcgaca atcaccgcac
    10081 aggtagagtc gtcaatcagc gcgctggcag agttaatatc gttatatgca gcatgacgga
    10141 tatctggtgg cagtggcgca aaatcctgtg aataggctgg ctgcccaccc gcactgacag
    10201 taaacagcgt gcgaccatga aacgcatttt taaacgccac gataccgctc ttatggctgc
    10261 cgtagcggtc gtgagcgaat ttacgcgcca gttttagcgc cgcttcgttg gcttccgcgc
    10321 cagagttaca aaagaagacg cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc
    10381 gcagtaccgg ttcgttggtg taaccgttgc cggtatgcca gaacttactc gcctgttcgt
    10441 tcagcgcctc gcgcagttcc gggtgcgcat gtcccagcgc gttcaccgca atgccacccg
    10501 cgaagtcgat atactctttc ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg
    10561 gtataaacgg tgccggagcg taaacaggta tcatccattc atcaaagttt tcacgcgtaa
    10621 ttggctgaga catagcgacc tctacagtaa ataattcgtt atttatatgt taataataag
    10681 taatatttgc gctgtaaatg tagattgcag ggttcgtgcc agccagtgat aaaagtgcat
    10741 aaaccgcgga ggctaactgg aaatcaagga gttataacca aaccatatgc atttaaagtg
    10801 catataaagt gaatacgttt gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta
    10861 tgcagaagta aaatataatt ctggaattgt gatcattgac gaaatttact ggaaattact
    10921 gcgccattct gacgcaacgc gcaccaaaag cgggcatttt ttgcgccatc gttgacatca
    10981 ttaacaacca tcgatcaaat cacttaacaa caggcggtaa gcaaagcgaa attctgctac
    11041 catccacgca ctctttatct gaataaatgg cagcgactat gaaatttgtc tcttttaata
    11101 tcaacggcct gcgcgccaga cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg
    11161 atgtgattgg cctgcaggag acaaaagttc atgacgatat gtttcccctc gaagaggtgg
    11221 cgaagctcgg ctacaacgtg ttttatcacg ggcagaaagg ccattatggc gtggcgctgc
    11281 tgaccaaaga gacgccgatt gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc
    11341 aacggcggat tattatggcg gaaatcccct caccgctggg taatgtcacc gtgatcaacg
    11401 gttacttccc gcagggtgaa agccgcgacc atccgataaa attcccggca aaagcgcagt
    11461 tttatcagaa cctgcaaaac tacctggaaa ccgaactcaa gcgtgaaaat ccggtgctga
    11521 ttatgggcga tatgaatatc agtcctggcg acctggatat tggcattggc gaagagaacc
    11581 gtaagcgctg gctgcgtacc ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg
    11641 acaggctgat gagctggggg ttggtcgata ccttccgcca tgcgaatccg caaacagcag
    11701 atcgtttctc atggtttgat taccgctcaa aaggttttga cgataaccgt ggtctgcgca
    11761 tcgacctgct gctcgccagc caaccgctgg cagaatgttg cgtagaaacc ggcatcgact
    11821 atgaaatccg cagcatggaa aaaccgtccg atcacgcccc cgtctgggcg accttccgcc
    11881 gctaatttag cagctctcct ggctcaaact gggtcaggag aattaacctt gagaaaaatc
    11941 aacaaactgt cagtaatgat ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt
    12001 tagttgtctg accacttctc tattatcaag tttgatatag gaaactccac gatgaacgct
    12061 gagcgtaaat ttctgtttgc ctgtcttatt tttgcgctgg tcatttacgc tatccacgct
    12121 ttcggtttat tcgatctgct caccgattta ccccacttac agacactcat tcgccagagc
    12181 ggattgttcg gttatagcct ctatattctg ttattcatca ttgccaccct ctttctgtta
    12241 ccaggaagca tactggtgat cgcgggagga atagtttttg gcccgctctt agggacacta
    12301 ctctcattaa ttgccgctac gctggcctcc tcgtgctcat tcctgctggc gcgctggatg
    12361 ggacgtgatt tactgctgaa atacgtgggc catagccata ccttcaaggc tatagaaaaa
    12421 ggcattgcgc gtaacggcat cgattttctt attctgaccc gcttaattcc gctgtttccg
    12481 tacaatattc aaaattacgc ttacggatta accgctattg ctttctggcc ctataccctt
    12541 atttcggcac tcacgaccct gcccggaatc gtaatttata ccgtgatggc aagcgatctt
    12601 gccaatgaag gcattacgct gcgctttatt ttacaactct gtctggcggg cctggcgctg
    12661 tttattctcg tccagctcgc caaactctac gcccgacaca aacatgtgga tctgtctgct
    12721 tcgcgccgca acccacttac tcacccaaaa aatgaaggat agaacgatgt tgcaacatta
    12781 ttcagtgtca tggaaaaaag gactgactgc actctgctta ctggctgttg ctgggcttag
    12841 cggctgcgat caaaaagaga atgcggcggc aaaagtggaa tacgacggac tttcgaacag
    12901 ccaaccgctg cgcgtcgatg ccaataacca tacggtaacc atgctggtgc aaattaatgg
    12961 tcgtttcctc accgacgaca ctcgtcacgg tattgtgttt aaagatggct ccaacggaca
    13021 taaatcgctg tttatgggtt atgcgacccc gaaagcattt tatgaagccc tgaaagaggc
    13081 aggtggtaca ccgggcgaaa acatgacgat ggataataaa gaaacgactc atgtcacagg
    13141 cagcaaactg gatatttcgg tcaactggca aggggcggca aaaacgtatt ccttcgatga
    13201 agtgattgtt gacagtaatg gcaagaaact ggacatgcgc tttggcggta atttaacggc
    13261 agcagaagag aagaaaacgg gttgcctggt gtgtctggat agctgcccgg tcggcatcgt
    13321 cagcaatgca acatacactt atggtgcggt tgaaaaacga ggtgaagtta aattcaaagg
    13381 caatgcctcg gttctcccgg cggataacac gctggcaacg gttatcttta aaatcaccga
    13441 ataaagccag gataaaggat gatgaagatg caatcgcgaa aaatctggta ctaccgtata
    13501 accctcatca tcctgttgtt cgctatgctg ctggcatggg cgctgcttcc cggcgtccat
    13561 gagtttatca atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg tatagaacgc
    13621 tttattcagt cttacggcgc actggcggca gttgtctcgt tcttgttgat gattttacag
    13681 gccattgctg caccgctacc tgcgtttttg atcacctttg ccaatgcgtc gctatttggc
    13741 gcgttctggg gcggcttgct gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt
    13801 tttatcgcca gagtgatggg ccgcgaagtg gtggaaaaat taaccggcaa aaccgtactt
    13861 gacagtatgg acggcttttt cactcgctac ggcaaacaca ctatcctggt ctgtcggtta
    13921 ttgccttttg tccctttcga tccaatcagc tatgctgccg gtttgacttc aatacgtttt
    13981 cgctcgtttt ttatcgccac cgggcttggt caattaccgg cgactattgt ttattcctgg
    14041 gcgggcagca tgttaacagg gggtaccttc tggtttgtca ccggactgtt tattctgttt
    14101 gccctgaccg tggtgatttt tatggcgaag aaaatatggc ttgagcgcca gaagaggaat
    14161 gcctgatggg tttaccgccg cttagcaaaa ttcctttcat tttacgtccg caggcgtggc
    14221 tgcatcgtcg ccattacggc gaggtgctaa gccccattcg ctggtggggg cggatcccgt
    14281 ttatctttta tctggtgtcg atgtttgttg gctggctgga gcgcaaacgc tcaccgctcg
    14341 atccggtagt acgatcgctt gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt
    14401 gtgtggatat caccagtatg aaagtcgccg agcgcaccgg cagcagcgat aaactgctgg
    14461 cagtggctga ctggcggcaa agcccgctct ttagcgatga agaacggctg gcgctggagt
    14521 acgccgaagc cgcaagcgta acgccgccaa cggtcgatga tgccctgcgt acccgactgg
    14581 ctgcgcattt tgacgctcag gcgctcaccg aactgacggc attgatcggc ctgcaaaatc
    14641 tgtcagcccg ttttaattct gccatggaca ttcccgctca ggggctgtgc cgtattccgg
    14701 aaaaacgttc ttaaggagag atgatgcgcc attgtgggtg gttgctggga ttgttatcgc
    14761 tgttttctct ggcaacacat gccagtgact ggcaagaaat taaaaatgag gccaaagggc
    14821 aaactgtctg gtttaacgcc tggggcggcg ataccgcaat taaccgctat ctcgactggg
    14881 ttagcggcga gatgaaaacc cattacgcta taaacctgaa gattgttcgt ctggcggatg
    14941 ccgcagacgc ggtgaagcgc attcagaccg aagccgcagc cggacgtaaa acgggcggct
    15001 cggtggatct actctgggtg aacggcgaaa acttccgcac cttaaaagag gccaatttac
    15061 tgcaaaccga ctgggcagag actctgccca actggcgcta tgtcgacaca cagctgccgg
    15121 tgcaggaaga tttttcagtg cctacagaag gggctgaatc gccctggggg ggcgcacaac
    15181 tgacatttat cgcccgccgc gatgttccgc cacagccacc acaaacgccg caagccttac
    15241 tggagtttgc taaagccaat cccggcacgg ttacctatcc gcgcccaccg gactttaccg
    15301 gcacggcgtt tcttgaacag ttgctgatta tgctgacggc cgatcccgcc gcattaaaag
    15361 aagcgccgga cgatgcgact ttcgcccgtg tcactgctcc cttgtggcaa tatcttgatg
    15421 cgctgcatcc gtatttgtgg cgcgaaggaa aggatttccc gccttcgccc gcgcggatgg
    15481 atgctctgct gaaatccggc acattgcgcc tgtcgctgac ctttaacccc gcgcatgcgc
    15541 agcaaaaaat cgccagcggc gatttgcctg caagcagtta cagttttggc tttcgcgagg
    15601 ggatgattgg caacgtgcat ttcgtcacca ttcctgccaa cgcgaatgcc agtgctgcgg
    15661 cgaaggtagt tgccaatttc ttgctctcac ccgatgcgca attgcgtaaa gcagatcccg
    15721 ctgtctgggg cgatccttct gttctcgatc cgcaaaaact gcctgatggg cagcgcgaaa
    15781 cattgcaatc aagaatgccg caagatctgc cgccagtact ggctgaaccg cacgcaggtt
    15841 gggtaaatgc gctggaacaa gaatggctac accgttacgg tacgcattaa tctttttgct
    15901 gtgggcgatg gtggcggtga tttatgcacc gctgatcccg gcagctctca cgctgatatc
    15961 gcctgctttg tcgttgacac actggcaggc gttatttgcc gatccacagt taccgcaggc
    16021 attactggca acgctggtgt cgacaaccat cgcggcggtc ggggcgttgt tgattgccct
    16081 gctgatgatt gtggcgctgt ggcctgggcc gaaatggcag cgtatgtgca cccgtctgcc
    16141 gtggctgctc gccattcccc atgtggcttt tgccaccagc gcccttctgc tctttgctga
    16201 cggagggctg ctttatgact atttcccgta tttcactccg ccaatggaca aattgggtat
    16261 tgggctgggc ttcacgctgg cggtgaaaga aagcgcattt ctgctgtgga tcttagcagc
    16321 ggtattgagt gaaaaacggc ttttacagca agtcatcgtg ctggattcgc tgggctacag
    16381 ccgctggcaa tgcctgaact ggctgctgtt gccctccgtc gcgcctgcgc tggcaatggc
    16441 gatgctggcg attgttgcct ggtcgctgtc ggtcgtggat gtggcgatta ttcttgggcc
    16501 aggtaatcca ccgacgctgg cagtaattag ctggcagtgg ttaactcagg gcgacgccga
    16561 tcaacaaacg aaaggcgcac tcgccagcct gctgctgatg ttgttactcg ccgcctacgt
    16621 tttgctgggc tatctgctat ggtgtacctg gcggcgtact attccccgcg tagatggcgt
    16681 tcgtaagcct gccacgcctt tattgccggg caatacgcta gcgagttttt tacccttgac
    16741 cggtgtgctg tgtgtaattc tgctggcgat cctcgcggat cagtcgacga tcaatagtga
    16801 agcgctcatc aacagcctga caatggggct ggtggcgaca ttcatcgctt tgctcctgtt
    16861 actgctgtgg ctggaatggg ggccacagcg tcgccagttg tggctatggt tgcccatttt
    16921 attaccggca ctgccactgg tggcgggcca gtacacgctg gcgctatggc tgaacctgga
    16981 taggagctgg acggcggtgg tctgggggca tctgctgtgg gtaattccgt ggatgctgtt
    17041 tatcctgcaa cccgcctggc agcgcattga ttcacggtta attttgattg cacaaacact
    17101 gggctggtcg cgggccaaaa tcttctttta cgtgaaatgc ccactcatgt tgcgccctac
    17161 gctgattgcc ttcgcggtgg gattttcagt cagtattgcg cagtatatgc cgacactgtg
    17221 gctgggcgcg gggcgttttc cgacgctcac cactgaggcg gtggcattaa gcagcggcgg
    17281 cagcaacggt attctcgccg cccaggcttt atggcaactg ctattaccgc ttattatttt
    17341 tgccctgacc gcgttagtcg caaaatgggt aggttatgtc agacaaggac tccgctaatg
    17401 ctctgcgtga aaaatgtttc gctacgttta ccagaaagtt gcttgctgac aaacgttaac
    17461 tttacggtgg ataaaggtga cattgtcacg ttaatggggc cgtctggctg tggaaaatcc
    17521 actctgtttt catggatgat tggtgcactg cccggacagt tttcttgtac aggtgagcta
    17581 tggctcaatg agcaacggat tgacatgcta cccaccgcac agcgtcagat tggcattctt
    17641 tttcaggatg cactgttatt tgaccagttc agtgtcgggc aaaatttact tctggcgcta
    17701 ccggcgacac ttaaagggaa tgcccgacgt aatgccgtga atgatgcact tgagcgttca
    17761 ggccttgatg gagctttcca tcaggatcct gccactttgt ctggcggtca gcgagcgcgc
    17821 gttgctctgc ttcgcgccct tctcgcccag ccaaaagcgt tactcctgga tgagccattc
    17881 agccgtcttg atgtggctct gcgcgataat tttcgccagt gggtgttcag cgaagttcgc
    17941 gccctggcga tccccgtcgt tcaggtaacg cacgatttcc aggatgttcc tgctgatagt
    18001 tctgttctgg atatggcgca gtggtcagaa aattacaaca aactgcgata acgcaaagtt
    18061 tttctcaatg cgtcagttca gaatggcgca ctcaaaacta caatgtcggg attttcgatg
    18121 aaacgtgttt ctcaaatgac cgcgctggca atggctttag ggctggcttg cgcttcttcg
    18181 tgggccgctg aactggcgaa gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa
    18241 gcgatagata ctcgccccag cgcgttttat aacggctggc cacaaacctt aaatggccct
    18301 tctggtcatg aacctgccgc cttaaacctc tctgccagct ggcttgacaa aatgagcacc
    18361 gaacagctaa acgcgtggat caagcaacat aacctgaaag ccgatgcccc ggtggcgctg
    18421 tacggtaatg gcaaagatgt cgacgccgtc aatacgcgac tgcaaaaagc aggttttacg
    18481 catatctcca ttctgagtga cgcgctaagc gaaccttccc gtctacaaaa actgccgcac
    18541 tttgagcagc tggtttatcc gcaatggctg cacgacctgc aacaaggtaa agaggttacg
    18601 gagaaacctg ccggagactg gaaagtcatt gaagcggcct ggggcgctcc taagctttac
    18661 cttatcagcc atattcccgg tgctgactac atcgatacca acgaagtgga aagcgaaccg
    18721 ctgtggaaca aagtttctga tgaacaacta aaagcgatgc tggcaaaaca cggcattcgc
    18781 catgacacca cggtcattct gtacgggcgt gacgtatacg ctgcagcgcg tgtggcgcag
    18841 attatgcttt atgccggcgt gaaagatgtg cgcctgctgg atggcggctg gcaaacctgg
    18901 tccgacgcgg gtctgcccgt tgagcgcggt acgccaccga aagtgaaagc ggaaccggat
    18961 ttcggcgtga agatcccggc acaaccgcaa ctaatgcttg atatggaaca ggcgcgtgga
    19021 ctgctgcatc gccaggatgc atcgctggtg agcattcgtt cgtggccaga gtttatcggt
    19081 acgaccagcg gttacagcta tattaaacca aaaggtgaaa tagccggagc acgttgggga
    19141 cacgctggta gcgactcgac gcatatggaa gattttcata acccggatgg caccatgcgc
    19201 agcgccgatg atattacggc tatgtggaaa gcatggaata tcagaccaga tcagcaagtt
    19261 tcattctact gcggcaccgg ctggcgcgcg tccgaaacct ttatgtacgc acgagcaatg
    19321 ggctggaata acgtctccgt ttacgacggc ggctggtacg aatggagcag cgatccaaaa
    19381 aatccggttg caaccggtga acgcggcccg gacagcagca aataacattg caattactga
    19441 cgctggagcg acttcagcgt cagataccca ctccacaccc gcgtaaaggt cgtcatccag
    19501 cacaacgcac caaataccca cgcaaaccac ggaaaacagg tcggaaagag gcaactcaga
    19561 acaaacagca aaatcgtctc gctgccttcg gttaacccgc ccagataata aaacgattta
    19621 tgcgcataac cgggattatc aatctgatgt ttcgctgcca gtgcagcaaa ggcgagaaaa
    19681 ctgctgcccg taccgataaa cgcaaacaac aaccagccgc ctgccagcgc attttgctcc
    19741 ggtgcagcaa gaataaaacc aaacggcacc agcgcgtaaa agagaaaatc cagcgagata
    19801 tctaaaaacc cgcctgcatc ggttaatcct ctgcgccgcg ccagtgcgcc atccagacca
    19861 tcaagcagac gattcaacaa aatgacgact agcgccgcca gataccagcc cagagccaga
    19921 aacggcagag ccagcacgcc gatggcaaat cccactaatg ttaaaccatc tggcgtaatg
    19981 cccggtttat caagaacccg cacgcactga tgcaacaacg gtttaatccg gggatgaaga
    20041 tggcggtcta gcacaggcac tccttaaata taaagccttt ctgattgagc aacagtgcgg
    20101 atattatggc atttttcgct tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt
    20161 tgccgccatc attgaacgtg atggcaaaat tttactcgcg caacgccccg cccagagcga
    20221 tcaggcggga ttatgggagt ttgccggtgg taaagtcgag ctggatgaaa gccagcagca
    20281 ggcgctggtg cgtgagttaa acgaagaatt gggcatcgaa gcaactgtgg gtgagtatgt
    20341 tgccagccat cagcgagaag tttcggggcg gattatccat cttcatgcct ggcacgtacc
    20401 cgacttccac gggacgttac aggcacatga acatcaggcg ctggtctggt gctcacctga
    20461 agaggcgctg caatatccgc tggcccctgc tgacattcca ctattagagg catttatggc
    20521 tttacgcgct gccagaccag cggattagtg ttaagggttt tgtcatcacg ctggcattgc
    20581 agcagtattc cctcggcttt aattaccgct ccttcagaat aatttttatc ctgataaaca
    20641 cagcactgag tacagggctg cgctgactgc ccgcctgaac tgaacacttc tggcggtacg
    20701 tttacctcca cgtccggacg ataatgcggg ttagccagtg caattaatgg aaatgctaat
    20761 actacggcga acaatgctcg actcacaggg aactccttaa cgttattgtc tctgctactg
    20821 ataacggtag ccgggtggca aaactttagc gtctgagtta tcgcatttgg ttatgagatt
    20881 actctcgtta ttaatttgct ttcctgggtc atttttttct tgcttaccgt cacattcttg
    20941 atggtatagt cgaaaactgc aaaagcacat gacataaaca acataagcac aatcgtatta
    21001 atatataagg gttttatatc tatggatcag acatattctc tggagtcatt cctcaaccat
    21061 gtccaaaagc gcgacccgaa tcaaaccgag ttcgcgcaag ccgttcgtga agtaatgacc
    21121 acactctggc cttttcttga acaaaatcca aaatatcgcc agatgtcatt actggagcgt
    21181 ctggttgaac cggagcgcgt gatccagttt cgcgtggtat gggttgatga tcgcaaccag
    21241 gtacaggtca accgtgcatg gcgtgtgcag ttcagctctg ccatcggccc gtacaaaggc
    21301 ggtatgcgct tccatccgtc agttaacctc tccattctca aattcctcgg cttcgaacaa
    21361 accttcaaaa atgccctgac tactctgccg atgggcggag gtaaaggcgg cagcgatttc
    21421 gacccgaaag ggaaaagcga aggcgaagtg atgcgtttct gccaggcgct gatgactgaa
    21481 ctgtatcgcc acctgggcgc ggataccgac gttccggcag gtgatatcgg ggttggtggt
    21541 cgtgaagtcg gctttatggc ggggatgatg aaaaagctct ccaacaatac cgcctgcgta
    21601 ttcaccggta agggtctttc atttggcggc agtcttattc gcccggaagc taccggctac
    21661 ggtctggttt atttcaccga agcaatgcta aaacgccacg gtatgggttt tgaaggaatg
    21721 cgcgtttccg tttctggctc cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa
    21781 tttggtgctc gtgtgatcac tgcgtcagac tccagcggca ctgtagttga tgaaagcgga
    21841 ttcacgaaag agaaactggc acgtcttatc gaaatcaaat ccagccgcga tggtcgagtg
    21901 gcagattacg ccaaagaatt tggtctggtc tatctcgaag gccaacagcc gtggtctgta
    21961 ccggttgata tcgccctgcc ttgcgccacc cagaacgaac tggatgttga cgccgcgcat
    22021 cagcttatcg ccaatggcgt taaagccgtc gccgaagggg caaatatgcc gaccaccatc
    22081 gaagcgactg aactgttcca gcaggcaggc gtactgtttg caccgggtaa agcggctaat
    22141 gctggcggtg tagcaacgtc aggcctggaa atggcacaaa acgctgcgcg cctgggctgg
    22201 aaagccgaga aagttgacgc acgtttgcat cacatcatgc tggatatcca ccatgcctgt
    22261 gttgagcatg gtggtgaagg taagcaaaca aactacgtgc agggcgcgaa cattgccggt
    22321 tttgtgaagg ttgccgatgc gatgctggcg cagggtgtga tttaagttgt aaatgcctga
    22381 tggcgctacg cttatcaggc ctacaaatgg gcacaattca ttgcagttac gatctaatgt
    22441 aggccgggca agcgcagcgc ccccggcaaa atttcaggcg tttatgagta tttaacggat
    22501 gatgctcccc agggaacatt tcttatgggc caacggcatt tcttactgta gtgctcccaa
    22561 aactgcttat cgtaacgata acacgcttca agttcagcat ccgttaactt tctgcgatag
    22621 cagcagatat gccagtaaag aaatcccatt agactatttt tttgataatc ttcttcgctt
    22681 tcgaacaact cgtgcgcctt tcgagaggca agcattatat aatgccaggc cagttcttct
    22741 tcaattgtcc cgttttgaaa agctgtgctt gatatcgaga tcatccatga taattccgcc
    22801 gcccatatta gcttcgccga ggatttaccg gagctatgat tagcgcaatc agagatatag
    22861 tctgagggaa aaacagcaaa tttattcaac aaggcgataa cctgctctgg ggcttcctcc
    22921 atgtttgctt taaaggtatt ggctccatgg tcgccagaaa gaaaatgctc cattaaggca
    22981 caataacttt cgctatcttc gataccccat tgatcctcta aagactcgcg tcttttactt
    23041 atgatatcga tcgagtcaaa aggaagcaca tgatattgga aggtatcttt gccaggttca
    23101 ggctttcgcg gccagaactc cagcgtttca gaccattgct tatgatagaa tcgataaggt
    23161 gcgatcaatt gtagcgcctg taacttctcg atactgagcg gctcaatacc tttagcctga
    23221 taataatgca gttgttcttt ttttgcttta aaaccggccc gaacaataag ccccatcgta
    23281 attaatagat aaagaaaaga gcatcccgcg gtaatcaagc ctctttcatt caaaccgttg
    23341 gatgttatcg ctgcgaatac aaacattaca gcgacaacac atgttaaata aaacccccac
    23401 tt
//
LOCUS       DAZGBC010000073        23309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGBC010000073 DAZGBC010000000
VERSION     DAZGBC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.073"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..137
                     /locus_tag="ACHK1J_002722"
     CDS             <1..137
                     /locus_tag="ACHK1J_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO7911967.1"
                     /translation="QQINGADYFIFGHMMLDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(178..414)
                     /locus_tag="ACHK1J_002723"
     CDS             complement(178..414)
                     /locus_tag="ACHK1J_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO7911968.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(425..1852)
                     /locus_tag="ACHK1J_002724"
     CDS             complement(425..1852)
                     /locus_tag="ACHK1J_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO7911969.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESSYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(1852..2445)
                     /locus_tag="ACHK1J_002725"
     CDS             complement(1852..2445)
                     /locus_tag="ACHK1J_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO7911970.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            2592..2999
                     /locus_tag="ACHK1J_002726"
     CDS             2592..2999
                     /locus_tag="ACHK1J_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO7911971.1"
                     /translation="MALRNKAFHQLRQLFQQHTSRWQHELPGLTKPQYAVMRAIADKP
                     GIEQVALMEAAVSTKATLAEMLARMENRGLVRRDHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(3119..4111)
                     /gene="rpoS"
                     /locus_tag="ACHK1J_002727"
     CDS             complement(3119..4111)
                     /gene="rpoS"
                     /locus_tag="ACHK1J_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO7911972.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(4174..5262)
                     /gene="nlpD"
                     /locus_tag="ACHK1J_002728"
     CDS             complement(4174..5262)
                     /gene="nlpD"
                     /locus_tag="ACHK1J_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO7911973.1"
                     /translation="MVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGT
                     TSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYS
                     GSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGN
                     AITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAP
                     VTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAII
                     ATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGS
                     TGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(5453..6079)
                     /gene="pcm"
                     /locus_tag="ACHK1J_002729"
     CDS             complement(5453..6079)
                     /gene="pcm"
                     /locus_tag="ACHK1J_002729"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO7911974.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(6073..6834)
                     /gene="surE"
                     /locus_tag="ACHK1J_002730"
     CDS             complement(6073..6834)
                     /gene="surE"
                     /locus_tag="ACHK1J_002730"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO7911975.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPAEQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(6815..7864)
                     /gene="truD"
                     /locus_tag="ACHK1J_002731"
     CDS             complement(6815..7864)
                     /gene="truD"
                     /locus_tag="ACHK1J_002731"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO7911976.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(7861..8340)
                     /gene="ispF"
                     /locus_tag="ACHK1J_002732"
     CDS             complement(7861..8340)
                     /gene="ispF"
                     /locus_tag="ACHK1J_002732"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO7911977.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(8340..9050)
                     /gene="ispD"
                     /locus_tag="ACHK1J_002733"
     CDS             complement(8340..9050)
                     /gene="ispD"
                     /locus_tag="ACHK1J_002733"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO7911978.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(9069..9380)
                     /gene="ftsB"
                     /locus_tag="ACHK1J_002734"
     CDS             complement(9069..9380)
                     /gene="ftsB"
                     /locus_tag="ACHK1J_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO7911979.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(9574..9897)
                     /gene="ygbE"
                     /locus_tag="ACHK1J_002735"
     CDS             complement(9574..9897)
                     /gene="ygbE"
                     /locus_tag="ACHK1J_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO7911980.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(9947..10552)
                     /gene="cysC"
                     /locus_tag="ACHK1J_002736"
     CDS             complement(9947..10552)
                     /gene="cysC"
                     /locus_tag="ACHK1J_002736"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO7911981.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(10552..11979)
                     /gene="cysN"
                     /locus_tag="ACHK1J_002737"
     CDS             complement(10552..11979)
                     /gene="cysN"
                     /locus_tag="ACHK1J_002737"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO7911982.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(11981..12889)
                     /gene="cysD"
                     /locus_tag="ACHK1J_002738"
     CDS             complement(11981..12889)
                     /gene="cysD"
                     /locus_tag="ACHK1J_002738"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO7911983.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            13141..14178
                     /gene="iap"
                     /locus_tag="ACHK1J_002739"
     CDS             13141..14178
                     /gene="iap"
                     /locus_tag="ACHK1J_002739"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO7911984.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   14199..14776
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=14260..14288
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(14873..15166)
                     /gene="cas2e"
                     /locus_tag="ACHK1J_002740"
     CDS             complement(14873..15166)
                     /gene="cas2e"
                     /locus_tag="ACHK1J_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO7911985.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(15163..16086)
                     /gene="cas1e"
                     /locus_tag="ACHK1J_002741"
     CDS             complement(15163..16086)
                     /gene="cas1e"
                     /locus_tag="ACHK1J_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO7911986.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGYIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(16083..16733)
                     /gene="cas6e"
                     /locus_tag="ACHK1J_002742"
     CDS             complement(16083..16733)
                     /gene="cas6e"
                     /locus_tag="ACHK1J_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO7911987.1"
                     /translation="MYLSRITLHTAQLLPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGRDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(16715..17461)
                     /gene="cas5e"
                     /locus_tag="ACHK1J_002743"
     CDS             complement(16715..17461)
                     /gene="cas5e"
                     /locus_tag="ACHK1J_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO7911988.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDHFHDLKVSLPKLQDECWWEGEHDGLVVSKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(17472..18527)
                     /gene="cas7e"
                     /locus_tag="ACHK1J_002744"
     CDS             complement(17472..18527)
                     /gene="cas7e"
                     /locus_tag="ACHK1J_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO7911989.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(18542..19078)
                     /gene="casB"
                     /locus_tag="ACHK1J_002745"
                     /gene_synonym="cse2"
     CDS             complement(18542..19078)
                     /gene="casB"
                     /locus_tag="ACHK1J_002745"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO7911990.1"
                     /translation="MSIVKEEHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(19075..20637)
                     /gene="casA"
                     /locus_tag="ACHK1J_002746"
                     /gene_synonym="cse1"
     CDS             complement(19075..20637)
                     /gene="casA"
                     /locus_tag="ACHK1J_002746"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO7911991.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPDVPGAQTTKFNKDHFIKRGVTEYLCPHCLALALFSLQLNA
                     PAGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPQKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDPDELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQNA
                     KAAREKKQEAAE"
     gene            complement(20734..>23309)
                     /gene="cas3"
                     /locus_tag="ACHK1J_002747"
     CDS             complement(20734..>23309)
                     /gene="cas3"
                     /locus_tag="ACHK1J_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO7911992.1"
                     /translation="LDVAAMGYLMVKRNCFGLADYFYQLGISDKEQAAQFFAWSLCWH
                     DIGKFAHSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLS
                     PRKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPA
                     FWDDDEGIELIKQLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNA
                     LTVFPLKAKVAPFTGINTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTE
                     AALILAHRLMAAGKAQGLFFGLPTMATANTMYDRLVKTWLSFYSPESRPSLVLAHSAR
                     TLMDRFNESLWSGDLIGSEEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAV
                     MPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQ
                     QRDKLVTAFARGTEGQQEAPLLEKDDYPWLTHVTQTDVNSHRVATRKDVERSVSVGWL
                     HSEQECIARIESSVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFS
                     DRQRIEMETLARFGKEDGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAG
                     RLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGRAMRNSAFVYPDHGR
                     IWLTQRVLRGQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRTMAKK
                     FVLNFRPGYAASINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVR
                     VRRSWWKKHRDEFSLLEGDAFRQWCIEQRQEPEMANVILVTDDESCGYSAMEGLTGKV
                     G"
BASE COUNT         5515 a         6418 c         5763 g         5613 t
ORIGIN      
        1 tacaacaaat aaacggcgct gattatttta tatttggaca tatgatgctt gataacattc
       61 agacgttcgc taaccagatt tatattgata ccggatcgcc gaaaagcggg cggctgtcat
      121 tttataaaat aaagtaatga ggccgggtaa agcaaagcca ctacccggca ctttttatta
      181 gcgcttacct tccgccagca gcggcgggat gctcggcacc attggcgcgt catcaatatc
      241 tttctgcgtc atgcggaacg cttcgggata atgttcgcgg ctggtacggc gcagcggttc
      301 ggtatcgcgc caggtataaa ggcaatgctg gcactgatat accgtccaga catctttcac
      361 cggcgatttc gccatcactt caatctgttc atcggcacaa cgtggacaaa tcatcttgtt
      421 ctccttattt acgtgcggcc agcatagcgg tcagtttttc agcccaggct ttggtttccg
      481 gcaaatccac caccggctgg ctgtagtgac cacggttgtc cggggcgaca ggcgtggtgg
      541 cgtcgataat cagcttgtcg gtgatccccg ccgggcttga gcctgggtcg agttccagca
      601 cggacatatt cggcaactgc accaaatccc ctgccggatt tactttcgag gagagcgccc
      661 acatcacctg cggcaggttg aacgggtcaa cgtcttcatc gaccataatc accatcttta
      721 cgtagcccag accgtgcggc gtggtcatcg cacgcaggcc caccgcgcgg gcaaagccgc
      781 cgtagcgttt tttggtggag ataatcgcca gcaggccgtg ggtgtacatg gcgtttaccg
      841 cctgcacttc cgggaactcg gctttcagtt gctgatacag cggcacacag gtggctggcc
      901 ccatcaggta gtcgatttcg gtccacggca tgccgaggta cagcgattcg aaaatcggcc
      961 tggtgcggta agagacttta tcgatgcgca ccacggtcat gttgcgcccg ccggagtagt
     1021 gcccggtaaa ctcaccgaac ggcccctcga tttcgcgttt acggctttcg atgacccctt
     1081 cgaggatcac ttctgaaccc cacggcacat caaaaccggt cagcggggcg gtggcgatcg
     1141 ggtacgagct ttcacgcagc gcgcctgcca tttcgtactc agattgatcg tatttcagtg
     1201 gcgttgcgcc catcagcgtg atgatcggat cgttacccag ggtgatggca atcggcagat
     1261 cttcaccgcg ctcttctgct ttatgcagat gcagggcgat atcgtgcatc ggcaccggtt
     1321 gcaggccgag cttacgcttg cccttcactt ccatgcggta gatgccgacg ttctgcttgc
     1381 cgaagttatc cgggtcgagc ggatcgcggg aaaccacgca cgctttgtcg agatagaaac
     1441 cgccatcacc gtcgtttaaa cgaaacagcg gcaggatatc gaacaggttg atctcgtcgc
     1501 catcgacggt gttctgcgcc caggctggat tggcgcggcg ctccggggcg atcgggaagt
     1561 tatcccagcg gcggataaac tcatcaatct gctttttgac cggagtgttt ggcggcaggc
     1621 cgagggaaat cgcgtggttc tgccaggaac cgatggtatt catcgccacg cgggcatcgg
     1681 taaaaccgcg aatattatca aaccacagcg ccggtgcacc atcgccgata cgcccggtgg
     1741 cgttggcagc ggcagccaga tccggttccg cgttcacctc ttcgctgatt ttcagtaact
     1801 ggccgtggtc atcaagcgcc tgtaaaaagc tgcgtaaatc atcaaatgcc attattcatt
     1861 ctcctgagaa aaattccggg cctgcggcaa tccttgccag cgcctggcgt ggggatgttc
     1921 gaggccaaat tgatccagca cgcgggctac tacatggtgg acaatgtcat ctaccgtttc
     1981 gggatggtta taaaaggcag gcatcggcgg caccatcgcc acgcccatgc gtgaaagtgc
     2041 gagcatattt tcgagatgga tggtgctaag cggcatttca cgcggcacca gcaccagttt
     2101 gcggccttct ttgagcacga cgtccgccgc gcgccctacc aggccatcag cgtaaccagc
     2161 gcggataccg gcgagcgttt tcatactgca cggaataacg atcatgccgt cggtacgaaa
     2221 ggaacctgag gagatggtcg ccgcctgatc cgccgggtta tggctgaagt cagcgagggc
     2281 agcaacatcg cgggcgctgt aaggcgtttc cagttcaatg gtggttttcg cccacttcga
     2341 catcaccaga tgagtctcga cattcggcat ctcccgcagc gcttgcagta atgccacacc
     2401 aagaggcgca ccggtagccc ctgtcatccc gacgatcagt ttcattgctt attctcttaa
     2461 ttgttcgtat acgaacgtta ttattaaaat acgcctctgt tctcatcgcg gcaagcactg
     2521 aaaagtaaca acatcccctt cgtcactgaa aaaccgctat gatagcggca gagaattagc
     2581 ccggagttta catggcgtta cgaaataaag cgttccatca gttacgacag ctttttcagc
     2641 agcacacatc tcgctggcag catgagctac ctggcctgac caaaccgcag tacgcggtga
     2701 tgcgcgccat tgctgataag cctggtatcg aacaggttgc gctgatggaa gcagcagtca
     2761 gcaccaaagc aacgctggca gaaatgctgg caagaatgga gaatcgtggc ttagtcagac
     2821 gtgatcatga tgccgctgac aagcggcgtc gctttgtctg gctgactgcc gaaggagaaa
     2881 agatacttgc tgcggcgata ccgattggtg acagcgtgga tgaggaattt ttggggcgtt
     2941 tgagtgcaga agagcaggag ctgtttgtgc agctggtgcg caagatgatg aacacatagg
     3001 atgcaaattg ccgggtagga cgctgacgtg tcttatccag gcgacaaaaa cacaagcttc
     3061 ccaaacaaaa gaaaaaggcc agcctcgctt gagactggcc tttctgacag atgcttactt
     3121 actcgcggaa cagcgcttcg atattcagcc cctgcgtttg caggatttcg cgcaaacggc
     3181 gcaggccttc aacctgaatc tggcgaacac gttcacgggt gaggccaatt tcacgaccta
     3241 catcttccag tgttgccgct tcgtacccca gcaaaccgaa tcgacgtgcc agtacttcac
     3301 gctgtttggc gttcagctcg aacagccatt tgacgatgct ctgcttcata tcgtcatctt
     3361 gcgtggtatc ttccggaccg ttctcttttt catcggccag gatgtccagc aacgcttttt
     3421 cggaatcacc acccagcggg gtgtctaccg aggtaatgcg ctcgttaaga cgaagcatac
     3481 ggctgacgtc atcaactggc ttatccagtt gctctgcgat ctcttccgca cttggttcat
     3541 ggtccagctt atgggacaac tcacgtgcgg ttcgcaggta aacgttcagc tcctttacga
     3601 tgtgaatcgg caaacgaata gtacgggttt ggttcataat cgcccgttca atcgtctggc
     3661 gaatccacca ggttgcgtat gttgagaagc ggaaaccacg ttccgggtca aacttctcta
     3721 ccgcgcggat cagccccagg ttgccctctt cgataaggtc cagcaacgcc agaccacgat
     3781 tgccataacg gcgggcaatt tttaccacca gacgcaagtt actctcgatc atccggcggc
     3841 gagaggcgac atctccacgc agtgcgcgac gcgcaaaata aacttcttct tcggccgtta
     3901 acagtggtga ataaccaatc tcaccaaggt aaagctgagt cgcgtccaac acacgctgtg
     3961 tggctccctg cgataacagt tcctcttcgg ccaaatcgtt atcactgggt tcctcttcta
     4021 ctaaggcctt ttcgtcaaaa acctcaactc cgttctcatc aaattccgca tcttcattta
     4081 aatcatgaac tttcagcgta ttctgactca taaggtggct cctacccgtg atcccttgac
     4141 ggaacattca agcaaaagcc tggttccgcc gatttatcgc tgcggcaaat aacgcagcgg
     4201 gtttacggat ttccccttgt aacgaatttc aaaatgcaag cgtgttgaac tggttccggt
     4261 gctacccatg gttgctattt tttgccccgc cttcacttct tgttgttccc ggaccagcat
     4321 tgtgtcgtta tgggcgtagg cactcaggta atcatcatta tgtttgatga taatcagatt
     4381 accgtagccg cgcagcgcgt taccggcata aacaacgcgg ccatctgcgg tcgcgataat
     4441 tgcctgtcct ttgctgcctg cgatatcaat ccccttgttg cccccctcag aagcgccaaa
     4501 ggtttcgatc actttgccct cagtcggcca gcgccaggtg gagataggcg tactggttga
     4561 tgtactgctg acagtcggct cggttgtgct tgctgttggt accgttacag gcgctgtgac
     4621 cgtggtcgca gttggcttgt tgttcggcaa cattttgtta gcactctgtt cacccgaaga
     4681 ctcagaatac gtaattgtcg gttgcgacgc aacagcaacg gtggaatttt gtgcaggctt
     4741 gatcacaact ccttgctctg ctgcgtcggc ctgggtaatg gcatttccgc cagtgattgg
     4801 cgtaccggaa gcattcccca cctgtaaggt ctgaccaacg ttcagcgcgt atggtgcctg
     4861 aatattgttg cgctgagcaa ggtcacggaa atcgttgcca gtaatccagg cgatatagaa
     4921 aagtgtgtcg ccttttttca cggtgtatgt actgccgcta taactgcctt tcggaatgtt
     4981 cccatactga cggttataga cgatgcgtcc gttttccatc tgtaccggct gctgagctac
     5041 tggctgcact ggctggattt gcggttgttg agtagcctga atttgtggct gctgtaccgg
     5101 ctgaatttgc ggttgctgcg ctgtagacgt cgtccccatt ttcggcggcg gcgtaatcaa
     5161 cataccagaa ttggtatttg caggcgcatt gccattgacg gaactgaccg gtgccggtgg
     5221 atttgaagtg tcagaacagc ctgccagcca tagcgaaacc agtgacaaag ccgcaatgcg
     5281 gcgaacggtg aattttgggc ttcccgcgct catttatccc ccaggaaaaa ttggttaata
     5341 accagtgaca taattaccgt gcaaggcacc atactgaaca ctggaaaaga tgttcacgat
     5401 acgctgacct gcggcaaaat aaccaggaaa aatccaggta tttcctcacg ttttaagcca
     5461 gctcaccctt cactaaaggg acaaagcgca cggcctccac ggtatcgata ataaattcgc
     5521 ctccccgacg acgcacccgt ttcaaatact ggtgctcctc ccctacgggt aagacgagaa
     5581 tcccgccttc gtccagctgc gtcattagcg cagttggaat ttccggcggt gccgccgtaa
     5641 caatgatagc gtcaaacggc gcacgtgcct gccaaccttg ccatccatcg ccatgacggg
     5701 ttgaaacatt atgtaaatca agatttttca gacggcgacg cgcctgccac tgcaaacctt
     5761 taatccgttc gaccgagcaa acatgctgga caagatgcgc caggattgcc gtttgatatc
     5821 ccgaaccggt gccaatttcc agcacccgcg actgcggcgt caactcgagt aattcggtca
     5881 ttcgcgccac catatacggc tgcgaaattg tctgcccctg acctatcggc aaagcgatat
     5941 tgtcccaggc tttttgttca aacgcttcat caacgaattt ttcacgcggc acggcggcaa
     6001 gtgcattcag cacctgctca tcctgaatac cttgcgcacg taattgatcc agaagtgctt
     6061 gtacgcgtct gcttaccatt gcgtgccaac tcccacgctg tttaaccagt ctgaaaccac
     6121 atcttgcgcg ctatgcgcag ttaaatccac atgcagcggc gtgatggaga catagccctc
     6181 atctaccgca gcaaaatcgg tccccggacc ggcatcacat ttaccgcccg gcgggccaat
     6241 ccagtacagc gtattgccgc gcggatcttg ctgcgggatc acctgttctg ccggatgtcg
     6301 tgtaccgcag cgcgtcacgc gaataccttt gatttgatcc aagggtaaat ccggaacgtt
     6361 aatattaaga atacgcccgg tgcgcagcgg ctctttacac agtgcgcgca aaattgaaca
     6421 ggttaccgcc gcggcagtgt cgtaatgttt atgcccgtca agcgagacgg caagcgccgg
     6481 aaaacctaaa tgacggcctt ccatcgcggc ggctaccgta ccggaataaa taacatcatc
     6541 ccccagattc ggcccggcgt taattccgga cacaacaatg tccgggcgcg gacgcatcag
     6601 agcattcacg ccaagataga cgcaatcggt cggggttccc atttgcacag caatatcacc
     6661 attttcaaag gtaaacgtgc gcagggagga ttccagtgtc agagaatttg aagcgccgct
     6721 gcggttacga tcgggggcga ccacctgaac gtcagcaaac tcacgcaagg ctttcgccag
     6781 cgtttgtata ccgggtgcat gtaccccgtc atcattactc agcaatatgc gcataatcac
     6841 ctgttgtgtt gataagttcc ctgacaacgc tggttgcaaa actacccgcc ggaagccaga
     6901 aacggatctc tacggtgacg tcatcccacc agttccagct taattgttgc ggatacagca
     6961 gcatcgctct gcgcgcggct tcaacttttt cgcgcaccag taaggtttgt aattcagttt
     7021 ctgcggcgac cgctgcttgt tcgaatgcca acgcttcacg ctgagttccc cattcgccac
     7081 tgcccggcaa tgcggcggtt atcatcagct ctttatcgtt gacgcgacgc tgtaattcca
     7141 ccagttcttc ggtggttgca acaaaccagc taccacgtcc ggctaattgt agcgcatcgc
     7201 cgtcaacaac ttgattaacg tctgcttttt tgaggcgctc agcaacaatc tgattaaaca
     7261 acgcactgcg ggctgccgac aaccaaaaac tccgtttatt gcgatcgcgc accggagtat
     7321 tggtttgcgc ccagcgcagc gcgccctgca agttgctacc gccaatccca aaacgttggg
     7381 caccgaagta gttcggtaca ccttttacgc aaatatcgat cagacgttgt tcaacgtcat
     7441 cgcgattgct cacttcgcgc agaaccaggg taaaggcgtt acctttcagc gcccctaaac
     7501 gcagcttgcg cttgtgccgc gcatactcca gcacctggca gccttccaat tgaaaggcgc
     7561 tcagatcggg catttccttg cccggcacgc gagcgcataa ccactgttct gtaacagcat
     7621 gtttgtcttt ttgcccagca aagctgactt cacgggcatg aattttcagg aatttcgcca
     7681 gtgcatccgc cacaaaacgg gtattgcagc cgtttttgag gattctaacc agaatatgct
     7741 caccttcacc atcaggctca aagcccaaat cttccaccac cacaaagtct tccggattgg
     7801 ctttcagcag cccggtgcct tgcggtttac cgtggaggta agtgagatta tcaaactcaa
     7861 tcattttgtt gccttaatga gtagcgccac cgcttcacag gcaatccctt ccccacgtcc
     7921 ggtaaatcca agtttttccg tagtagtggc tttcacgtta acatcatcca tatggcagcc
     7981 gagatcttcg gcaataaaca cgcgcatttg tggaatgtgc ggcaacatct tcggtgcctg
     8041 agctatgata gtgacatcga cgttgccaag ggtataaccc ttcgcctgaa tacgacgcca
     8101 ggcttcgcgt agcagctcgc ggctatcggc acctttaaat gccggatcgg tatccgggaa
     8161 cagtttgccg atatccccca gcgccgccgc gccaagcaat gcatcggtca acgcatggag
     8221 cgccacgtcg ccatcagaat gcgccagcaa tcctttttcg taaggaatgc gtacgccacc
     8281 aatgataatt gggccttcac cgccaaaggc gtgtacgtca aaaccgtgtc caattcgcat
     8341 tatgtattct cctgatggat ggttcgggtg aggtaaaact cggccagtgc caaatcctcc
     8401 gggcgcgtga ctttaatgtt atccgcacgg ccttcgacca actgaggatg gaatccgcaa
     8461 tattccagcg ccgaggcttc gtcggtaata gtcgcgcctt catttagagc gcgcgtcaga
     8521 cagtcatgta acagctcacg agggaaaaat tgcggcgtca gcgcgtgcca taagccgttg
     8581 cgatcaacgg tatgagcaat ggcatttttg cccggttcgg cacgtttcat agtatcgcgc
     8641 actggtgctg ccaggatccc tcccgtgcgg ctggtttcgc tcaacgccaa caatcgcgcg
     8701 aggtcatcct gatgcagaca aggacgagcg gcgtcatgca ccaataccca ctgcgcgtcg
     8761 ccagcggctt tcaaacctgc cagcacggaa tcggcacgct catcaccgcc atctacaacg
     8821 gtgatttgcg gatgattcgc cagaggaagt tgtgcaaaac ggctatcgcc aggacttatg
     8881 gcaatgacga cacgtttcac ccggggatgc gccagcagcg catgcaccga gtgttcaaga
     8941 atggtttgat taccgattga gagatattgc ttaggacatt ccgtttgcat tcgacggcca
     9001 aatccggccg ccggaaccac ggcgcaaaca tccaaatgag tggttgccat gttaattccc
     9061 gggctgattt atcgattgtt ttgccccgca gactgtgcgc gcttcgacgc gtcaggtacc
     9121 agacgataaa aagtttcgcc cggcctggtc atgctgagtt cattacgcgc acgctcttcg
     9181 agcgcctcct ggccgccatt gagatcgtca atttcggcaa aaagttgatc gtttcgcgct
     9241 ttaagtttcg cgtttgtagc ttgctgtgcc gccacatcat cattgacgcg ggtatagtca
     9301 tgtataccgt tcttaccgaa ccacagcgaa tactgtagcc agaccagaat agccagcaac
     9361 agcagcgtta gtttacccat cctgccccct gaaaaacggc atcatcatcc catgcatccg
     9421 aagacgactc tacatcctct gttggggata ccgcgacaac gcgggcaaat gtaccacatt
     9481 tgtccattgt tacgtatacc cagggcgtgc agaacataat ctcattatta gttacggttt
     9541 gaattatgaa cagaggagac aagaaagtac aaattagccc agtagccaca taaacagtgc
     9601 gccaaacata atgcctactg tcatcagggt gaaaacaata ctgtagcgta gctttccgtc
     9661 catcaatgaa tgcagcgcaa tccccaccac taccgcaacg ggcatcagcg ccagaaagaa
     9721 aggccaggtg tagataaaga agaacagcgt gttagagcca taaatcaaca tcggcatcgc
     9781 cagcgcaaat aaccaagaga taaaaccgac cacggcacca ggcagtgacc atgtggtttc
     9841 ttcatcctca gtaaggctgt cgttatttgt tagtgtaatg ttatggctat tacgcatatt
     9901 tgatcctgtt actttgacga accgggcatg gaaacccggt ggtgtctcag gatctgataa
     9961 tatcgttctg tctcaacaga tctaataatt gctgtaccaa atttgttact aattgttcac
    10021 cattgagatg aatttctgcc gattcaggcg cttcgtaaac ggaatctatt cccgtaaagt
    10081 tgcgcagttc accggcacgc gctttcttat ataagccttt tggatcgcgg gcttcgcaaa
    10141 tcgccagcgg cgtatcgaca aacacttcga taaagcgccc ttctcctacg cgttcgcgaa
    10201 ccatctggcg ttcggcgcgg tgtggcgaga taaatgcggt cagcaccacc agtccggctt
    10261 caaccatcaa attcgccact tcaccgacgc gacggatatt ctctttacga tcggcatcgc
    10321 taaaaccgag atcgctgcat aatccgtggc gaacattgtc gccatccagc agatacgtac
    10381 tgacgccgag tttatgtaac gcctcctcca gcgccccggc gaccgttgat ttaccggacc
    10441 cggagaggcc ggtaaaccac agcactacac cacgatgacc gtggtgtagc tcgcgttgtt
    10501 gcacagtgac cggatggcta tgccagacga cgttttcgtc atgcagcgcc attatttatc
    10561 ccccagcaaa tcgcgcgcgc cccagtgtgg aaagtggcgg cgaaccaggg cattcaattc
    10621 cagttcgaat gcactaaatt cagatggcgc agcagttgcc tggctaactg gctcatgcac
    10681 cataccggca cctacggtca cattgctcag gcgatcgata aaaatcagcc caccggtaac
    10741 cgggttttgc tgataacgat ctaacaccag tggctcgtca aaagtgagat ccaccaggcc
    10801 gatgccgttc agcggcaggt tttcaacttc gcgttgggta aggttattaa tatcaacctg
    10861 ataacgaatg ccatcaacac gagcacgcgt cttcttaccg gcaattttga tgtcgtaact
    10921 ctggcccggg gaaagcggct gttccgccat ccataccaca tccaccgacg cgctctgcac
    10981 agctggtaac gcttcgtctg ccgccagcag cagatcgcca cggctgatgt cgatctcatc
    11041 cgtcagcacc agggtgatag cttctccggc aaaggcttct tcgcgatcac catcaaaagt
    11101 cacgatccgc gcgacgtttg attccacacc agagggcagc acttttacac gttgcccgac
    11161 ttccacgcga ccggatgcca gcgttccggc gtagccacga aaatcgagat ttgggcggtt
    11221 aacgtactgc accgggaagc gcattggctg ggcatccacc actcgctgaa tctccacggt
    11281 ttccagcact tcgagcagtg tcggaccgct gtaccacggc atactttcac tttgcgaagc
    11341 cacgttgtca ccttccagtg cggagagcgg cacaaagcgg atatccagat tacccggcag
    11401 ctgcccggca aaggtcagat aatcttcacg aatacgggtg aacgtctctt cactgtaatc
    11461 caccagatcc attttgttga tcgccacgac cagatgtttg atccccaaca gtgtggagat
    11521 aaaactgtga cgacgggttt gatcgagcac gcctttacgg gcatcgatca gtaagatcgc
    11581 cagttcacat gtcgatgcgc cagtcgccat attgcgggtg tactgctcgt gccctggagt
    11641 gtcggcgata ataaatttac gcttctcggt agagaaatag cggtaggcca cgtcaatggt
    11701 gatgccctgt tcacgctcag cttgcaggcc gtccaccagc agagccagat ccagcttttc
    11761 gccctgggtg ccgtgacgct tactgtcatt atgcagcgat gagagctgat cttcatagat
    11821 ttggcgggta tcgtgcagca gacgaccaat cagggtactt ttgccgtcat cgacgctacc
    11881 acaggtcaga aaacgcagca ggcttttatg ttgttgcgca atcatccagg cttcgacgcc
    11941 gccttcattg gcgatttgtt gtgcaagtgc ggtgttcatc ttaaaaatac ccctgacgtt
    12001 ttttcagctc catagaacca gcctggtcgc ggtcaatcac gcggccctga cgttcactgg
    12061 tggtggaaac cagcatctct tcgatgattt ccggcagtgt ttgtgcattt gactccaccg
    12121 caccggtcag cggccagcag cccagcgtac ggaaacgcac catccgtttt ttgatcactt
    12181 cgcccggttg caggtcgata cggttgtcat caatcatcat caacataccg tcgcgttcca
    12241 gaaccggacg ttccgcagcg agatacagcg gcacaatgtc gatattttcc agccagatgt
    12301 attgccagat atcctgctcg gtccagttag agagcgggaa gacgcggatg ctttcgcctt
    12361 tgttaatttg cccgttgtag ttgtgccaca gctccgggcg ctgatttttc ggatcccagc
    12421 gatggaagcg gtcacggaaa gagtagattc gctctttagc acgggatttc tcttcgtcac
    12481 ggcgcgcacc accgaaggcg gcatcaaaac cgtatttatt cagcgcctgt ttcaggcctt
    12541 cagttttcat aatatcggta tgtttcgcgc tgccgtgcac gaatggatta atccccatcg
    12601 ccacgccttc cgggttttta tgcaccagca gctcgcagcc gtaggctttc gccgtacgat
    12661 cgcggaactc atacatctcg cggaatttcc agccggtatc aacatgcagc aacgggaaag
    12721 gcagcgtacc tggataaaac gccttgcgcg ccagatgcag catgacgctg gaatctttac
    12781 cgatagagta gagcatcacc ggatttgaaa attctgccgc cacctcgcga ataatgtgga
    12841 tgctttccgc ctccagttgc cgcaggtgag taagtcgttt ttgatccata accgttcctt
    12901 tgcaataccg ctattttctt gccatcagat gtttcgacta tagggagcgt aagagaacga
    12961 atgaaattac caattagaat gagtagttcc ttaacggaat aacgatttgg caaagctaat
    13021 atcaaaaagt gcttaaggca ccggatttcg ggcgtttagg aagatttgaa attgttttag
    13081 cgcagcggca gtttcatact atggcggtaa aaaaatttgc atggtattta aggactcact
    13141 atgttttccg cattgcgcca ccgtaccgct gccctggcgc tcggcgtatg ctttattctc
    13201 cccgtacacg cctcgtcacc taaacctggc gattttgcta atactcaggc acgacatatt
    13261 gctactttct ttccgggacg catgaccgga actcctgcag aaatgttatc tgccgattat
    13321 attcgccaac agtttcagca aatgggttat cgcagtgata ttcggacatt taatagtcgg
    13381 tatatttata ccgcccgcga taatcgtaag agctggcata acgtgacggg aagtacggtg
    13441 attgccgctc atgaaggcaa agccccgcag cagatcatca ttatggcgca tctggatact
    13501 tacgccccgc tgagcgatgc tgacgccgat gccaatctcg gcgggctgac gttacaagga
    13561 atggatgata acgccgcagg tttaggtgtc atgctggaat tggcagaacg cctgaaaaat
    13621 acgcctaccg agtatggtat tcgatttgtg gcgaccagcg gcgaagagga agggaaatta
    13681 ggcgctgaga atttactcaa gcggatgagt gacaccgaaa agaaaaatac gctgctggtg
    13741 attaatctcg ataacttaat tgttggcgat aaattgtatt tcaacagcgg tgtaaaaacc
    13801 cctgaagcag taaggaaatt aacgcgcgac agggcgctgg caattgcgcg tagtcatgga
    13861 attgccgcaa cgaccaatcc gggtttgaat aaaaattatc cgaaaggcac tgggtgttgt
    13921 aatgacgcag aaatattcga caaagcgggc attgctgtac tttcggtgga agcgactaac
    13981 tggaatcttg ggaataagga tggttatcag caacgcgcaa aaacacctgc cttcccggcg
    14041 ggaaatagct ggcatgacgt aagactggat aatcaacaac atattgataa ggcacttcct
    14101 ggaagaatag aacgtcgctg ccgtgatgtt atgcggataa tgctaccgtt ggtgaaggag
    14161 ctggcgaagg cgtcttgatg ggttggaaaa tgggagctgg gtgttctacc gcaggggcgg
    14221 ggaattctca gtgatatcca tcatcgcatc cagtgcgccc ggtttatccc cgctggcgcg
    14281 gggaacacat atttttgtcg gtcgcctgga agttcagttt cggtttatcc ccgctggcgc
    14341 ggggaacacg gattcagaac gccgtcatcc tccgcaatcc acggtttatc cccgctggcg
    14401 cggggaacac aattcatcat cagattcacg gtcagcgcca ggcggtttat ccccgctggc
    14461 gcggggaaca catccagcgc gaccattttt ccctgtaaat tttcggttta tccccactgg
    14521 cgcggggaac acctatcttg cgacagctaa atattccaac aaagcggttt atccccgctg
    14581 gcgcggggaa cacaaagtgc tgaaggcgct taatgctctc gctggcggtt tatccccgct
    14641 ggcgcgggga acactcatac agcagggtgc cgtcaccgtc acgcaacggt ttatccccgc
    14701 tggcgcgggg aacacttgca atctgatggg gcagctttct tcaatttcgg tttatccccg
    14761 ctggcgcggg gaacacacta agcatacata tctgttttta aacaacttta ttccacatca
    14821 acaatctacc aactaaattc aaacatttcc ttatttttaa agaacacata acctattgat
    14881 tatcaacagg aagaaaagaa accaaacgta acccatccaa atccaccgga atacgtctgt
    14941 tttctcccca ggtctgaaat tcaaaacccg actcggtatt ggtcgcccag gccatcacca
    15001 catttccgca accagccagt tgggtaattt gctgccagat catctcccga atacgttttg
    15061 atgtatcacc aacatacaca ccggcacgca cttccagtag ccagattgcg agccgtccac
    15121 gtaagcgcgg agggacattt tctgtaacaa ccacgaccat gctcatccgc cgcgcccccg
    15181 gtgaccacta tcacccagcg tttcaggttc agggatggca ggcggtaaca tatccggcgc
    15241 gggttgtggt ggttcaattt cacctgcagc aaggacttcc tcaattaacg gtattaattt
    15301 gcccgttaac ttagtgctac ggaaaatatc gcgacaggct aatctgactt ctttatcagg
    15361 ttctgcgggt tgcctcgctg ctatttcaaa tgcctttggc acaaccgaat caaatttaat
    15421 gatatcggct atgtcataaa caaatgaaag cggtttgcca ctatgaatat atccaatagc
    15481 gggcgcatat cccgcggcta atactgccgc ttcagaaata ccgtacagac atgatgtggc
    15541 agcactgatg cagcgattca caacatcgcc tttttcccag tctttaggat cgtatttgcg
    15601 accattccat ttcacaccat attgtttcgc cagtaatgca taggtctggc gaacgcggga
    15661 tccctcaatt ccccgtagct gatccactga acggcgagct ggcggtggct cacgaaaacg
    15721 taattcatac attttgcgca ccaccttcag gcgtagatct tccgttaaag ccagctttgc
    15781 ctggtagagt aatttatctg cccgcgcccc tccgggttgt ccggaagagt aaacgcgaac
    15841 gcccgcttca ccgacccaga ccagcagtgt tcccaccgtg gcggccagat gcaccgccgc
    15901 gtgggaaact ctcgttcccg gttcgagcat aatgcaggcg accgatccca ccggaatgtg
    15961 cgtgcggatc ccggttttgt cgatcagcac gaaagcgccg tccagtacgt cgatttgacc
    16021 gtactggagg aagatcatag aggtgcgatc ttttaacggg atcggactca gtggtacaaa
    16081 cgtcacacct ctgctccggg tttgatcagc atcagaccac agccgaacgc gcggcttttg
    16141 ccatacccct gactgagacg ttgcagaaat aacccgggat cggtgaccgt aagcatcccc
    16201 gtataatcca cgctactgaa ctggatcagt tgccgggagt tttcccgccg cagttgctgt
    16261 tgtcggtagg catcaaccga ggtatccagc agtgtaaaac cgcttctctc cccctgcgct
    16321 gccagccagt ccagcgccgc ctgttgttga tgcaaccaga catcacgccc ttccgcctgt
    16381 cccctcacct gccgtttcgc ctccatcagc agatcgtggc gcttgcccgc tttacaaatt
    16441 gtgggattcg cccgcaggtt gaaacaaagg gattgtccgg tacgcagctc cggagcaaat
    16501 gaccggcatt cgatagtgaa agtttcactt tccgccgggc gttcctgcga gaggacaaaa
    16561 aagcgaaacg caccctgaag ttcctcccga cgataaagaa attgcctttc tttgccgcta
    16621 gggaagagat cccacagcca ctgatgcatc acatattcac cgcgatccac cagatgcagc
    16681 aactgcgcag gcaaaagctg agcggtatgt aaggttattc ttgagaggta catggttcct
    16741 ccttgctgag ccacggcccc tgattgacgg tgcgttcccc aaacagccac tgctggcgat
    16801 ttaaaggaac atcccggcgg cgtaatattt tgctcacgac caggccgtcg tgctcccctt
    16861 cccaccagca ttcatcctga agtttcggga gtgagacttt caggtcgtgg aaatgatcct
    16921 gatactgttg gtatgcgtta cgtaaaacat cagacgcgtt gccttcgagc agtaacggcg
    16981 caagtggtaa tgccagcgga tgactttttc gccccagata aagaggaaaa actggatggc
    17041 gtaaaccgcc ctgcaactgt tcgaggctgt aaggcgcatc ggctgttgtt gccaccgcca
    17101 ccatccacca ggcgtcggtg tagtagtcgc gccgggagat aatcgcgctc agaagatcag
    17161 ggtcgctcaa ctcttcgcga cggctgaaat aacgcgcttt acgcacctct tttggcatct
    17221 ggatcgtgtg ataatcccgt gcccagcgcg ggttacggct ggcgcaaacc accagtgaat
    17281 agtggcgatt aaacgcgttt aagcgttcgg tatcatcacg ccgaatccct accccggcgg
    17341 ccagcagacc cagcaatgct gagcgcgaag gcagttcatg ggtatgacgc acttcgccgg
    17401 gggcatcgac gccccaggat gccattggcc catgaagctg aaaaatcaga tattggctca
    17461 taagccgccc cttacgcgca gataaagtcc agcacgtcct tcatgcttcc ctgtttgttc
    17521 atcacgtcaa aacttgcgca ttcggtcttc tgttcgtaga ccgtattcat attttcgcga
    17581 agcgttgtaa tacgctgcac cgccacgtct aactgccggg tgccattaat gggttcatag
    17641 aaggccgcag ccagagaacg cggttgatcg gtgccttttt ccgccagcgc ccaggaagca
    17701 taggcacggc tggcaaagct attttgttta ccggttgggg aaactttaag cgcggcttcc
    17761 gtaaaagcgc gtatggtctg attagctaac gcttcgtcac cgccgaggtt ttctaccagc
    17821 agatctttat caatgcagat ataggtgtag aaaagtgctg aaccgaatcc ggtttctccg
    17881 agatgcccgg caccggcatc ttcagaggcc tggcgcaaat catcaacggc ggtgaaaaaa
    17941 tcatcttcga caatcgtttc actgacacca aacgcatgcg cgacctggca ggcggcttca
    18001 acattaaact caggtttatt cgccagcata cgaccaaaca tagcgatatc taccgctata
    18061 cggtctttac gtaacaacgc gagatcttcc tcttttggcg cgcgcttttc ttctgccagt
    18121 tgatgggcca gcgcttttac ggcgtcaaat tctgccgggc tgatatgaac taattgttca
    18181 gtttcggcgt tagtgagcgg atcttttggt tttttgtcgt ttttagcttt cccaagataa
    18241 tccgcaattt ttgccgccca ttcgatggct tttttctctt cgattccttt ctcaatcagg
    18301 atagttgccg cctcacgcgc aattcgccca ctgcgaatac caatatggcc cgccagtgcc
    18361 tgttcaaaaa gtgcagaagt gcgccacgca cgtttcagac tttgtgagga aacgcgcaga
    18421 cgcgttgcac cacccagtac cacagttttt ggcgcaccag tatcatcacg gttaaggttg
    18481 gcagcagggt aagcggttaa caaatgaagc tgaataaatg ttgtcatgag atagtccttt
    18541 attaattttg ttcgttatcg gcgtcgccag cctggtaata ttccagtgcc cagcggatac
    18601 ggataaattc ggttggccgc tgctggcggc ggtgatgatt gagcaggtcg tcgctctcct
    18661 ggcaccagcg gaaaatatct tctgccaaag agataagatt gacggaaccg tttaataatt
    18721 tgaccgcgcg acgtaactga cgcagtaaat catccggcgt ttttactgcc gacaggcgag
    18781 taaaacgccc ctccgacatt accgccgcca gctgcgcggc aaaaggctgc cgttcatcaa
    18841 tggcttttac attggcgcta actgcggcga ccagcgccag tgcggtaaaa cgccactctt
    18901 gtgcttcaat tttccacaaa gtgtgggtct gcatcagcag agaacgaaac ccttctgaca
    18961 agcaaacatc attaaccgtt gtgctacgcc gcagactggc acgattgccg cgtttagcct
    19021 gcaactcttc gtgccattta cgcagcgtgg ctttgtgctc ctcttttaca atactcattc
    19081 agcagcctcc tgctttttct ccctggcggc tttagcattt tgcttctccg ccgatgttgt
    19141 aaaatatttc ttgcgtgcgg tcatgacgcg tttcaaatca acgggctcat aaggattggt
    19201 gaatacacgc tcgtcaaaat cctgacgcgc aaataaccaa atttcctttt gccatttgcc
    19261 gagtaattca tctggatcct gaccttcttc aatttggcgc actaacctca ggaagcgatg
    19321 ctgagttttg ttccagaagt cgatatccac aaaactgaaa tcaccccgtg caccttttgg
    19381 atcggagaac cacgcttctt tcaatgcact ccgtaacaga ctcagaatcc gtgaagccgt
    19441 ttgcgcagcc agccgcagtt tcggtatctg gccttctttt ttattgagca gaagcgggaa
    19501 atggtgctcg taccaacagc gcgctttcat gttgtcaaaa tcataaccaa atccccacag
    19561 gcccactttt gcctgtttca gactgctggc attaaagagt ttcaccacca gcgcgggaag
    19621 ttccgtattg ttttctgatt tccccgtttc gataaggcct aaccagtcgc gccagattaa
    19681 accgcccggt tgtggtttaa ctgagtaaaa ctcgccgccc tctttaagtg gtacacggta
    19741 aggcgttaag ggatgctgcc acatggcata gttagcgccg taatttttgg tcgtcatcag
    19801 gctcagaagc gcgtcgctct gctcaccgca aatatcgcag ttgccgactg tggtggtatt
    19861 aaaatcaata cgaatacgcc gtggcattcc ccagtacgcc tggagtttat taacctgatc
    19921 atcggttacc accgcaccgg ccagttcgct ggtacgcgtc gggccaagcc aggggaaaac
    19981 cagatcgtca aatttttggg gtagcggtaa gtcggcttca tcctgcggca tcacgttgag
    20041 ccacagtttg cgccataagg gtgtttgttg attgccctga tattcctgta attcaatcag
    20101 agtggtcatc ggcccaccgc cgcgcaaacc ggtgcgatag cctttgccac ccgctggtgc
    20161 attcaactgt aaagagaaca aggccaatgc taaacaatgg gggcacagat attccgttac
    20221 accacgcttg ataaaatggt ctttattaaa cttcgtcgtt tgagcgccgg gaacatcagg
    20281 cagtagcgaa gcgacctgaa ctttatctcc cgtgagcgcc tcaaaatcct gcataaatga
    20341 aggggaatct gggccaaact ggaaagcatg ttctaatgac agcaatgctt cccttagctt
    20401 ttcagcttcc agtccgtctt cccatatatc atcccaacga cgatgatctt ttggcgcaaa
    20461 gctggtttgt agtagcccca gcaaaaactg ccatgccgca ccttgtaaat ctgctcgcgg
    20521 cgcggcgata tcgacaacat tttcatcagc cagatcgact ggtgccagct tgcctgtcgt
    20581 tccgtcttta taacgaacgg gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg
    20641 catcctctcg taaaaagacc atccctggtc ggttgtccat ccttccatcc ttttcggtgt
    20701 atcaactatt cagagagccc tgtcgcaggc atctcaacca accttgccag tcaatccttc
    20761 catagccgaa tacccgcaac tttcgtcatc ggtgactaaa atcacgtttg ccatctccgg
    20821 ctcttgccgc tgttcaatgc accactgcct gaacgcatcc ccttccagta aagaaaactc
    20881 atcccgatgt tttttccacc agcttcgacg cactctgaca acgctcattt cccatgcgtg
    20941 agcaccggta gcataaggct tcaccacacc gtcaatacag gtagccagcc acagggaaac
    21001 agactcttca gccagacgtg tcgacagttt ttccggaagg taatcgttga tactggcggc
    21061 atagccgggc ctgaagttca gcacaaactt tttagccatt gtgcgatcgc agtaatattt
    21121 gcccacttgc tcctgctcgc tgcgggcaaa tccttccggc attaccacgt cctcaccgta
    21181 aactgattca ataagaagcc gggctgcgtg tggcatctga atagcgcctt gcccacgcag
    21241 cacacgctgc gtcagccaga ttcgcccatg atcggggtag acaaatgcac tgttccgcat
    21301 ggctctgcca aaccattcat cacccggaga gtcgtcccag acgggtgcca gaatcagcaa
    21361 ttcaggaggg gaacgctcgt cttttccgtc acgctttaac tgaccattaa tatcgcgaat
    21421 atgccgctgt aatcgccccg ctcgctgaat cagcaaatca acaggggcca ggtcggagat
    21481 catttcgtcc aggtcacaat caacgctctg ctctaagacc tgagtacaaa tgaggacttt
    21541 tccggcacgc tgtgaaccgt cttctttacc aaagcgtgcc agcgtctcca tttcaattcg
    21601 ctggcgatcg ctaaaagcaa agcggctatg aaagagtgaa aggctggaag cgggaatgac
    21661 gccgcgggca agtaactgac gatgaacttt aatagcgtca tcgacagaat tccggatcca
    21721 ggcgatgcat ttcccctgac ttaccgaaga ttcgatacgc gcaatacatt cttgttcgct
    21781 atggagccaa cccacgctca cgctacgctc aacgtctttg cgcgtcgcta cccggtgcga
    21841 attcacatcc gtttgcgtga catgcgtcag ccaggggtaa tcatcttttt ccagcaatgg
    21901 agcttcttgc tggccctctg tgccacgcgc aaaggcggta acaagtttgt cgcgctgctg
    21961 ttgagataac gtggcagaaa gcaaaataac gctgtttcca ccacgcgcct gtcgctcgat
    22021 cagcccttca agaatgcaag acatgtaagc gtcacaggcg tgaatctcat cggccagcag
    22081 gattttgtta ctcagcccca gaagccgcag attattatgt ttaaacggca tcaccgccat
    22141 catcgcctga tccagggtgc cgacgccaat ttcagccagc aacgccttct tgttactgtc
    22201 ggcaaaccag gccgcacaac cctggctgaa agcttgttca tccggttctt ctgacccgat
    22261 taaatcaccg gaccagagtg attcattaaa gcggtccatt aatgtgcggg cactgtgtgc
    22321 cagcaccaga ctggggcgag attctggcga atagaaagaa agccaggttt tgacgagccg
    22381 atcatacatg gtattggccg ttgccattgt tggcaggcca aaaaacaaac cctgagcttt
    22441 acccgcagcc atcaacctgt gcgcaagaat aagcgccgct tctgttttac ctgcaccagt
    22501 cacatcttcc agaataaata actgtggccc aggctggctg atatccagat ccagcacctt
    22561 ttgctgtaac ggtgtcggat tctcaataaa aggaaacagc gtattaattc cggtgaaagg
    22621 cgcgactttt gcttttagag gaaagacggt taaggcgttt tgagcctgaa ctaaagtttt
    22681 ctgccagtaa cctttaatat ccattgggtg tgcgacgcgt ggaaaaaatc gcgttgacga
    22741 acctgtccag tctgcgagta cgacggttgc agagatatac caggaaagtt gttttataag
    22801 ttcaatgccc tcgtcatcat cccagaatgc ggggatctct attcgcggaa acagtgcctt
    22861 gattgcaagg agaaaatctc gtgctgcagc tttgtcttca ggcagaaaat tatccaattc
    22921 atccatacga tcagggggcc gaccatgatg ccctgtcgtc acgggcatcc acatttctat
    22981 cactcgccta agtttgcgcg gagagagcga agatgaagga agtaactcct gacattcact
    23041 taaataatga ttccacaacc agtagcccag cgttgaatga gagatttttt cgtaattttt
    23101 tctggcacct tcctgaatct tgagttcagg gggcaggtaa agttgttgaa aagagtgggc
    23161 aaattttcca atatcgtgcc agcacagcga ccaggcgaaa aattgagccg cctgttcctt
    23221 gtcagaaatc cctaattgat aaaaataatc agccagcccg aagcaatttc ttttaaccat
    23281 taaatagccc atcgcggcca catccagcg
//
LOCUS       DAZGBC010000074        22810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGBC010000074 DAZGBC010000000
VERSION     DAZGBC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.074"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1133
                     /gene="gap"
                     /locus_tag="ACHK1J_002748"
     CDS             132..1133
                     /gene="gap"
                     /locus_tag="ACHK1J_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7911993.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVNGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1175..2614)
                     /gene="aldA"
                     /locus_tag="ACHK1J_002749"
     CDS             complement(1175..2614)
                     /gene="aldA"
                     /locus_tag="ACHK1J_002749"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO7911994.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISSGIRECASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2811..3611)
                     /gene="ydcF"
                     /locus_tag="ACHK1J_002750"
     CDS             complement(2811..3611)
                     /gene="ydcF"
                     /locus_tag="ACHK1J_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO7911995.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTIFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPHVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            complement(3883..7785)
                     /gene="hrpA"
                     /locus_tag="ACHK1J_002751"
     CDS             complement(3883..7785)
                     /gene="hrpA"
                     /locus_tag="ACHK1J_002751"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO7911996.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7986..8591
                     /gene="azoR"
                     /locus_tag="ACHK1J_002752"
     CDS             7986..8591
                     /gene="azoR"
                     /locus_tag="ACHK1J_002752"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO7911997.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8645..9937)
                     /gene="ynbD"
                     /locus_tag="ACHK1J_002753"
     CDS             complement(8645..9937)
                     /gene="ynbD"
                     /locus_tag="ACHK1J_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO7911998.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATIMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCLRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(9951..11708)
                     /gene="ynbC"
                     /locus_tag="ACHK1J_002754"
     CDS             complement(9951..11708)
                     /gene="ynbC"
                     /locus_tag="ACHK1J_002754"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO7911999.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLIATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(11724..12620)
                     /gene="ynbB"
                     /locus_tag="ACHK1J_002755"
     CDS             complement(11724..12620)
                     /gene="ynbB"
                     /locus_tag="ACHK1J_002755"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7912000.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGVLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMV
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(12620..13225)
                     /gene="ynbA"
                     /locus_tag="ACHK1J_002756"
     CDS             complement(12620..13225)
                     /gene="ynbA"
                     /locus_tag="ACHK1J_002756"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7912001.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(13396..15702)
                     /gene="ydbD"
                     /locus_tag="ACHK1J_002757"
     CDS             complement(13396..15702)
                     /gene="ydbD"
                     /locus_tag="ACHK1J_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO7912002.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALKIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIASMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWGFLESALISWDNLPAVVLKELQQNTSR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(15766..16626)
                     /gene="pdxI"
                     /locus_tag="ACHK1J_002758"
     CDS             complement(15766..16626)
                     /gene="pdxI"
                     /locus_tag="ACHK1J_002758"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO7912003.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDILDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(19519..22266)
                     /locus_tag="ACHK1J_002759"
                     /pseudo
     CDS             complement(19519..22266)
                     /locus_tag="ACHK1J_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
BASE COUNT         5722 a         6099 c         5235 g         5754 t
ORIGIN      
        1 cattaaatat tgttcacccg ttttcagtac gttacgtttc actgtaagaa tggagattgc
       61 ccatcccgcc atcctggtct aagcctggaa aggatcaatt ttcatccgaa cgttcctgac
      121 aggagaaaac catgagtaaa gttggtatta acggttttgg tcgtatcggt cgactggtgt
      181 tgcgtcgatt acttgaagtc aaaagcaaca tagacgttgt cgctattaat gatctcactt
      241 ctccaaaaat tctcgcctac ctgctgaaac atgattcaaa ctacggaccg ttcccctgga
      301 gcgttgattt tacggaagat tcacttatcg ttaatgggaa aagtatcgcg gtttacgccg
      361 aaaaagaggc taaaaatatt ccgtggaaag cgaaaggtgc agaaatcatt gtcgaatgta
      421 ctggctttta tacctccgcc gagaaatcgc aggcgcatct tgatgctggt gcgaagaagg
      481 tgttgatttc cgctcctgcc ggtgaaatga aaaccatcgt ttataacgtt aatgacgaca
      541 ctctggatgg caacgatacc attgtttccg tggcgtcatg caccactaac tgccttgcgc
      601 cgatggccaa agccttgcat gacagtttcg ggatagaagt cggcacgatg acgaccattc
      661 atgcctatac tggcacccag tcactggtgg atggcccgcg tggtaaagat ttacgtgctt
      721 cacgcgcagc ggcagaaaat atcattcccc acactacggg cgcggcaaaa gccattggtc
      781 tggtgatccc ggaactgagc ggcaaactga aaggtcatgc gcaacgcgtg ccggtgaaaa
      841 caggttcggt cactgaactg gtatcgattc tcggaaaaaa agtgactgcc gaagaggtga
      901 ataacgcact taaacaagca accaccaata acgagtcatt tggttatacc gatgaagaaa
      961 tagtctcttc cgatatcatt ggcagccatt tcggttcggt gtttgatgcc acgcaaacgg
     1021 aaattaccgc cgtgggcgat ttacaactgg tgaaaacggt cgcctggtac gataacgaat
     1081 atggcttcgt cacgcagctt attcgcaccc tcgaaaaatt cgctaaactc tgacgcgcac
     1141 aggcggagga aaaaacctcc gcctctttca ctcattaaga ctgtaaataa accacctggg
     1201 tctgcagata ttcatgcagg ccatgtttac catctgcgcc gccaataccg gatttacgcc
     1261 atccggcgtg gaagccttgc atagcttcga agttttcacg gttgatgtaa gtttcaccaa
     1321 acttcagccc tttaatggct ttcatcgcga cgttcagatt ttgggtatag attgatgagg
     1381 tcaggccgta atcactgtca ttagccattg agatagcatc ttccagcgtg tcgaatgcga
     1441 caactggcag caccgggcca aaggtttcct catgcataat cgacatttcc tggcgaacat
     1501 ccagcagcaa tgtcggcgga taataatatc ctttcccctc taccgctttg ccacccaacg
     1561 ccactctcgc cccttcttct actgcgcgcg ccactttttg ctcgaccctt tccagcgccg
     1621 cggcgttaat caacggcccc atcgcaatgt cgttgcgttc agcggggtta ccaaattgaa
     1681 ccgcctgcat cgcttcaccc agccgattga cgaactgatc ataaatgcct ttctgtacat
     1741 aaacacgttc tgcacagtta cacacttgcc cactattaat gacgcgtgaa tcaacgatgg
     1801 ctttgactgc cagttcaaga tcggcatcgt ccattacgat agctggtgct ttacccccca
     1861 gttccaggca cactttggtg atgtttttcg ccgcagtcgc catgatcttc tcacctgcag
     1921 agacgctgcc tgtcatactg accattgcga cctttgggtt acccgccagt tcttgcccaa
     1981 cggtttcacc acgccccaga acaaggttaa acacgccgcg cggaaggcct atttcatcga
     2041 cgattttggc gaatgcaatc gcattgtttg gcgtaaattc actgggttta atgacgatgg
     2101 tattaccggt caaaagagcg ggagccattt tgcgggcaat gaggaagaac gggaagttcc
     2161 acggcagaat gccagtagtc acgccaagtg cacgtttaaa cagaagaata ttttctcctg
     2221 gacgatcgct ttgaataatc tcgccctcgt aacgccgtgc ccactccgcc atgtaatcga
     2281 tatagtccgc agtaaaagcc acttcgactt cagccagctg ctggatcttg cccccttctt
     2341 caacaatcag cgcactgatt tcactggcgc attcgcggat cccggaggag attttgcgca
     2401 accaactggc gcgttcaatg gcaggcaacg cttcccattc tggttgtgca cgttctgctg
     2461 catcgattgc cttacgagca tcctcggcct gaccatcggg aatgcgggaa atgacagcct
     2521 ctgtagcagg gtttaccaca tcaatccatg cgtctccacg ccaggtaaca aactgtccat
     2581 caatatacat aggatgttga acgggtactg acatgggcga ctcctgtgat ttatatgttt
     2641 gtttttggta aatacattgt taattaacat caatcatcag agagacggca tcgtgaaagg
     2701 acaattctgt gaagggcttc ataaaaacaa gtgtaaaagg aataagaggc agaaataaaa
     2761 ggcaggagtt atgcccgttt gcaaacaagt ggttcacaaa cgggcaattt ttaacgtaac
     2821 gagcgacttt ccatcgcctc gatgagcacc gcatcgtgtt taagcgtttg ccatgcctga
     2881 atgacttctg ccggaaaatc aacgtgaacg ataaaatctc gcccgcgggg accgtagcca
     2941 tcgctatcat cgcgtaaacg cggcagctcg ccagtgagta gtgagagata acgctcaacc
     3001 ggccataatc cttgtaactg attaataaag attacactgt ctgcgttatt tcctaactga
     3061 ggaacgaatc cgggataact taaccagcgt ggtacatggg gattgtcccc ggtcatacgg
     3121 cggaacgttg ccatcgtgcg ccgctgcatg gtggggtcct gtacaacgat agccgtatga
     3181 actcgttcta cggcctgatt caatagcgcg atgctaaagc gtgcgttttc accgcagttt
     3241 gttgactggt cttcaatcca gattttttca tgcggaatat gccagaactg atgagcgata
     3301 tccgccagga tggtcgcttc tgctctgcca gtggtgcgga tagtgttgta gtgcggatgc
     3361 tgtgcgatgg cgctatacaa aaaaatggtc gagtgaccga taccaccact aatcagtaaa
     3421 ggaatttgct gatcgcgggc aatcttacat gccgcatcta tagtcggcat aaccgcattg
     3481 cctgcaagta tgacgcaatc ggcctgatac ggcacctcac cggagaaatc atcctgcgcc
     3541 agccactgtc cgataacatt tatggcatct atagttgccg tcgaaagcgt cggaaacggg
     3601 gtaatgttca tggttcctcc ttatcgttcc cttaagaata ctgcgtgaga taatagtgag
     3661 aagcggagca ctctgaaagg aggatgtcgt atttccttaa ccgccactcc gcaagcctga
     3721 catatctttt aaggaaatgc tgtagataaa cattattcat cttgcagcag agaattgtat
     3781 ttttctgaat tcctgaattt ttgcccttac cgttttagaa ttaataaaaa acccggcgct
     3841 aaatgcttac cgggttttat atttatcagg caaatagcag cgttaaccgc taatctgctc
     3901 catcgcctgc aaaatacgct tatctgaaat cggataaggc gtaccaagtt gttgagcgaa
     3961 gtaactaacg cgcagctctt ctatcatcca acggatctct ttcacgtctt catcctcacg
     4021 acgtgcaggc ggcagtttgt tgatccattg ctgccacgcc tgctggacgt tttcgacttt
     4081 cagcatctgt gcacgatcgc gatgtggatc aaccgccagc ttttccagcc gtttttcaat
     4141 cgcctgcaaa taacgcagcg tgtcgcccag ccgtttgaag ccgttaccag tgacaaaacc
     4201 gcgatatacc aacccgccca tctgcgcttt aatgtcagaa agccccagcg ccatggtcat
     4261 atccacccgc cctttcagac gcttgttgat attgaacact gccgtaagga tttgctcgac
     4321 ctgcttcgca atatccacca ccgtgtcgtt cagttcggca cgcacttttt catgcagcgc
     4381 agcaaagcct tcttccgtcc agaccgggcc accattggcg tcgatcaatt tatccacacc
     4441 gcaggagata cagtcgtcga tcagctccag cactttgcca tacgggttaa agtacagtcc
     4501 aagcttggct ttgtttggta acttctcatg taaatacttg atcggcgatg gaatattcag
     4561 cagcagtaga cggcgaagac cgttccacat tgcctgtttt tgctccagcg ggttatcaaa
     4621 cagtttgatc gccacactgt cgcgctcatc caccagcgcc ggccacgcct tcactttgta
     4681 gttgccgcgc ttctgctcgt agctttccgg cagttgccca aaactccaga tatgtaagcc
     4741 gctctgctcg ataccgtcat ccgccaccgc agacagcgtt tcctgcactt tgcctttcag
     4801 cgcatctttc agatcttgta gcgagcgccc ttcttttagc ttcttgtttt tgtcatccac
     4861 cacgcggaag gtgattttca ggtgatcggg cacctgatcc cagtgccagt cttcgcggtc
     4921 aacggtaacg ccggtcatcc gccgtaactc gcgctcaagg ctgtcaagca acggtaactc
     4981 cagcggtgtg acgcggccta aaaacgcttc ggcgtagttt ggcgcgggta caaaattacg
     5041 gcgtaccggt ttcggcaacg atttaatcag agcaatcacc agttcgcggc gcagaccggg
     5101 gatctgccat tcaaacccgc tttcctcaac ctggttaagt aacggcagcg gaatatgtac
     5161 ggtcacaccg tcagcatccg cgccgggctc aaactgatag ctcaaacgca gcttgagatt
     5221 gccctgatgc cagaagttcg gataatccag cttgctgatc ttttccgccc cttctttgat
     5281 caacatgctc ttttcaaagt tgagcaaatc aggcgtttcg cggctgactt ttttccacca
     5341 actgtcgaag tgacgagcgg agattacatc gtggctgatg cgctggtcgt agaactcaaa
     5401 caacgtttcg tcatcaacca gaatatcgcg gcgacgtgat ttgtgttcca gctcttccac
     5461 ttcggcccgc agtttcaggt tttcacggaa gaatgcgtga cgcgtctgcc agtcaccttc
     5521 caccagcgcg tggcgaataa agagttcacg acataacgcc ggatcgatct ggctgtagtt
     5581 gaccttgcgc gcggcaacaa tcggcaaacc ataaacagtg actttttccg ttgccatcac
     5641 cgcgccctgc gcccgttccc agtgcggttc gctgtaggta cgtttaatca aatgctgagc
     5701 gacaggctcc acccattccg ggtcgatacg cgcagcaatg cgcccccaca ggcggctggt
     5761 ttctaccagt tccgccacca ttacccattt cggcggtttt ttgaataaac cagaaccggg
     5821 gaagatggag aaacgcgcgt tacgtgcgcc ggtatattct tgtttatcgg catctttcat
     5881 gccgatatgg gaaagcaaac ccgtcagcaa cgcaatgtga atttcacgat actccgccgg
     5941 ttcgctgttc accggaatgc cgagttcttt caccacctgg cgcaactggg tgtagatatc
     6001 ctgccattcg cgcacacgca gatagttgag ataatcggta cgacacaggc gacggaaggc
     6061 gttggaagaa agcgcctttt gctgctcgcc aagataattc cacagattca caaacgcgag
     6121 gaagtcagac tctttgtcgt ggaagcgacg atgtttttcg tccgatgcct gctgtttgtc
     6181 catcggacgt tcgcgcggat cctgaatgga gagcgcagac gtgataatca tcgcctcacg
     6241 cacgcagcca tgtttttgcg cttccagcac catacgcgcc agacgtgggt cgacaggcaa
     6301 ctgcgagagc tggcgaccga gcggcgtcag tttataggcg ctggcctgtt catccgtggt
     6361 gatcgcgccc agttcttcga gcagacgcac gccatcctgg atattgcgtt tatccggtgc
     6421 ttcgacaaac gggaacgcag cgatatcgcc cagccccagc gcggtcatct gcaaaataac
     6481 cgaggccagg ttggtacgca gaatctccgg atcggtaaac tccgggcgcg agaggaaatc
     6541 gtcttcggaa tagagacgaa tacagatccc ttcggacaca cgaccacagc ggcctttacg
     6601 ctgattggca gacgcctgcg aaatgggctc aatcggcaaa cgctgcactt tggtgcgata
     6661 gctgtagcgg ctgatacgcg ctgtaccggg gtcgataacg tatttaatcc ccggtacggt
     6721 cagcgacgtt tccgcgacgt tggtcgccag cacaatgcgc cgtccgctat gcgactggaa
     6781 caccctattc tgttcgctat tcgaaagccg cgcataaagc ggcaagattt ccgtatgtcg
     6841 taagttcagt ttgttcagtg catcagcggt atcgcgaatt tcccgctcgc cgctcataaa
     6901 gatcagaatg tcgccggggc tttcctggct cagttcgtct acggcgtcaa aaatcgcctg
     6961 caattgatcg cgctcggtgt catcggcttc ttcaacaatc gggcgatagc gcacttccac
     7021 cggataggtc cgaccggaga cttcgataat cggcgcatta ttaaagtggc gcgaaaagcg
     7081 ttccgggtcg atagtcgcgg aagtgataat gatttttagg tcaggacgcc gcggtagcaa
     7141 ctctttcaaa tagccgagca aaaaatcgat attcaggctg cgttcgtgcg cttcgtcaat
     7201 aatgatagtg tcgtactgca tcagcaggcg gtcttgctgg atctccgcca gcaggatacc
     7261 gtcggtcatc agcttgacca tcgtgttatc acttacgtga tcgctgaaac gcactttgta
     7321 accgatgcaa ccgcccggct ccgttttcag ctcttccgca atacggtttg ccactgttct
     7381 tgccgccaga cgacgcggct gggtatgacc gatcagccct ttaataccgc gccccagctc
     7441 catacagatt ttcggtagct gagtcgtttt accggaaccg gtttccccgg cgacgatcac
     7501 cacctggtga tcacgaatcg cttcgagaat gtcctgcttt ttctgactaa ctggtaaatt
     7561 gtccgggtaa gtaatttccg gtcgtgccgc ttcacgcagc aggactttcc ctgccgcctg
     7621 gtcaatctct ttcgccatct cctggaaaat ggcctgttgt gcatcaggat ttttaacctt
     7681 cttcacacca tgcagacggc gagaaaaacg cagtctgtca cgcagcatca gcgaatccag
     7741 ccgctgctgc aaggctgtaa aggtcaattt ttgttgttct gtcataacgt tagagggcag
     7801 taggctgcct gattaatttt cttttaaatg atagatatag agtaccacat cgcggctttc
     7861 tatgcgttgt tcaataaatt cgaacatagg cttcgattta ttgcgctatc tctgtggcag
     7921 gattgtgaat aaagtgtcag caagcaacgg ggcatcgccc attcaaacat ctataaggaa
     7981 acaccatgag caaggtatta gttcttaaat ccagcatcct ggcagggtac tctcagtcta
     8041 atcagttgtc cgattatttt gttgaacaat ggcgcgaaaa gcactccgct gatgaaatca
     8101 ccgttcgcga cctggctgca aatccgattc cggtactgga tggcgaactg gttggtgcgc
     8161 tgcgtccgag cgatgcgccg ctgactccgc gtcagcagga agctctggca ctttccgatg
     8221 agctgattgc cgagctgaaa gcccatgacg ttatcgttat tgcggcaccg atgtataact
     8281 tcaacatctc gactcagctg aaaaactatt ttgacctggt tgcacgtgca ggcgttactt
     8341 tccgctatac cgagaatggt ccggaaggtc tggtaacggg taaaaaagcc atcgttatta
     8401 ccagccgcgg tgggatccac aaagatggac caacggacct ggtgacgccg tatctgtcca
     8461 cgttcctggg ctttatcggc attactgatg tgaaatttgt cttcgccgaa gggatcgcat
     8521 acggtccgga aatggcagcg aaagcgcagt ctgacgcgaa agcagccatc gacagcattg
     8581 ttgctgcata accggacacg gtcttgatgt ggactgtaga tattcagtcc acatctcaat
     8641 ccacttacct gttttcccac aatctcagca ttgctttgtg ttcgtctgtc agcacaatct
     8701 gcgggcgtct ggctcgaata tagctaatcg cttcattaac ggttttacag tgtccgtaac
     8761 ataacaacca tgccgccacc accagcgcac tgcgcgataa tcccaatgca caatggacca
     8821 gaacgctgcc ttgctcttcg cgtaatgttt ccagcatcgc cacggcctgt cggagctccc
     8881 cctcttccgg aaccaccaga tccagcatcg gtacacaaaa atagagtcga tcttttgtgg
     8941 ctcgtccgcg agggaattca aaggtgacgt ccagaaccgc attctgtgcc ggaatatgtc
     9001 gtggaaacgc ccctaaataa acaccagcag tcattttgct caccggctcc aggcgaagac
     9061 aaaaccaacg catagacagc cacatcccga tgcgccaggg caatgtcagc cagtaaacgg
     9121 cgggcgggag tttcccctga ctatctttcc ctgttgttat cgcgccaagc ccaccgtacc
     9181 cacggccaat gatgaatagc gataatactg gccaacataa ccagactgac caccataact
     9241 gaagcattat cattagctcc atcaacacaa tgcacgagca cgcgcctacg acatatggca
     9301 gtgctatttt gattcgacgt tgatcaggtt tctgataatt ccaacgacgg tcgacgggca
     9361 ccatccagtc aattaacata cctaccgcca gccctgtgat gacatcaata aaatgatgct
     9421 gccaggttgt cagcgtcgaa atggcgatga gtaaaaacca tccgccgcag actttacgcc
     9481 acctcacagc cagatgctga cgaaagtgac gccaaagtag ccagcagaga ataatatgca
     9541 gcgaaggaga ctggttataa ggcagatcaa acagttcaag ttgcgaaaat agccatcctg
     9601 tcaccccact cacttcagga cggataaaac taaacttcag cggatagaga aaaaaaccgc
     9661 agcaggccat tatcgttgcc agaataagcc ggtgaaccag tcggcgctgt tcgaatgtcg
     9721 aactacaaac gaacaacgaa aacccatata aaagatccag actccagtaa ggaacaatag
     9781 tccaggggag aaaagggatc gccgtttccc agccgaatac ctgactgggg atatcatggc
     9841 tgttaaagtc ctgaaccgcg gtgaactgat taagagatcc ataggtgaag aagaaaaacg
     9901 gggccagcaa caataaccag ccagcgcctt gtagcaatac gttccggcgt tcagttatca
     9961 cgacgcaccg ccatcgaaac cgtaaaaatg ccccactcat caatccgttg agtgcattta
    10021 tcaaatccgg cattacgcac gagcgaatcc atctcccctt gcgaacgcac gcgcattacc
    10081 cacggtttac catctttatg actggttaac accccggcaa tcagctccag ttgtgggtgc
    10141 cacggctgcc cggtgtagat gagaatgcca cccggttcga tggcattggc aagacctgcg
    10201 agtgagtttt ttacctgctc atttccggga aaaagctcat acagtccaga aacaatcgcc
    10261 agcgtaggcc gcggagttaa cgcgctgagt tcctccgggt taaacgcatc gccctgttca
    10321 aaacgcaccc gcccagacat tccccgttgg gcaatcatct cttgcccctg agcaacattt
    10381 aactcactgt aatcacgtaa caaaatatcg cttacggcag gttcgtttgc cagcgcatcc
    10441 agtacatagc gcccatgccc tgcggcaatg tcaaccacgc ggacggctaa acctttggcg
    10501 tggagatcgg caacggcttg tttaatcagt atttgtaaat gggttttgcg ctggcgaata
    10561 ccgcgccagc caacactgtt caggtagatt ttgtcgatta agcgcccgaa tgcgttacta
    10621 ccttgcggtt gattttgata gacataatcc agcgaactgc ctgaatcaaa gccggtgctc
    10681 attccgagat gcaggcccgc agagtgcgtc ccgaacaatt tcatcgcttt acgcataaag
    10741 cgatacgcca aatcaaccgg cgataatggc acgggtccac ctgaaaggag ccgccagcga
    10801 tccgctgatg gtccagtgcg gtcttcatgc tgataatcaa atttttgtga cttgttagca
    10861 tataaacgac taataaagct ttgcattttt tcaaatgcct gcgccctgtt ctcttcaccc
    10921 aacgtgtcgt gataaaagcc tggcagcaga tgcagctctt tcagagggct acgtaatctc
    10981 tgataaaaat caatctgcgg ttggcgatgc accacatagt catcgcctga tatcagaagt
    11041 tgcgtgggga gcgtaatcgc cgccgcatcg ctaacaatac gttcagacgt tttatagaga
    11101 tctagcaaga tattgacggc aatcgccctt gtgatcagcg gatcattatt gaaactcgcc
    11161 acccgttgcc gatcgtgggt caaatagcgt cctttcacat aggaattaat aaaaaacaga
    11221 ccacgcagac gatgccataa cgccagcgca ggacgtgcca gcggcacata caatttaacc
    11281 ttaaaggccg gagaagccag caccagcccg cgaattgcag gcgcataatc atgaacccat
    11341 gtggcaatca gcactgcgcc gacgctttgc gcgatcacta ccacctcttc cagtccaacc
    11401 tggctgtcgc tggcagcaaa acggacaaat tcatcgacat cccgcactga acgcgcaaga
    11461 gatgggctat aaccacgcgg ccccgaagtt tttccatgcc ctcgggcatc ccatgcataa
    11521 aaagcagtat ctggcatcgc cagttcatca acgagatgtt gtagacgacc agaatgttca
    11581 tgaccgcgat gaaataagac gatgaccttt ttcgccccgg gctgtaaagc gggccagtgc
    11641 cgataaaaca acgctgtatt atcactggtg gtaaaaaaat gttccccagg gatgcgtgaa
    11701 ttttccattt tctctatttt ccttcagtaa cagcagtagc gaataaaata aaaaaatacc
    11761 ggagcggtga aaattaatga gtcaatcctg tcaaggagtc cgccatgtcc tggtaatagt
    11821 tttccgctat ctttaacacc aatatctcgt ttgatggctg acatcactac atcgccgcaa
    11881 aaaccactaa taccaattaa caaacccgct aataatgcct gtaatgtatt tagcggtgtc
    11941 agcaacggcc caataatcag tgacgcaacc atggtggtga ttacgccccc caccagacct
    12001 tccagcgttt ttcccgggct gactttgggg accacttttc ttctgccgca ggatttcccc
    12061 cataaatact gtgcaatatc gtttgactct gttaacgcca aaagaaataa caccagtaac
    12121 gccccgccct gggtatttgt ggttggcaac attaataacc aggcggcatg actcaaggcg
    12181 aaaacggtca ttatccagcc ccaaaaaatg gcgctcacgg tatgcagaaa accggaggta
    12241 tcacctacta aaactcgcca ggtggttaat atcagaaccc cagccaaagg tataaataac
    12301 agaaacagct caaagcaatt aaaaccgata agcaaatagt taagaggaat aacccaatat
    12361 aaccaacgcg gaaaatgtac ggatataagc gtacaatatt ctttcaatgc cagaaaactt
    12421 atcagcgcaa aaaacgtcaa cgtcatccag cgtggacctg aaatcaccag tgaaaaacaa
    12481 atgacgatgc cccaccaggt acgaatacgt aatgtaactt catccccttt tctctccggt
    12541 aaccgccata cccgaaaacg gtttatcagc gttgctaaaa ttaacaacgc gaaaagtgtt
    12601 gccagagatt tttccagcat tatctttcag cactcatcag gagcacactc cgacaacgat
    12661 taatcgcagt ccagagaagc agaattgaag caatgctcca taaaagatta ttccattgca
    12721 tccactgtgg ataaatggca acggccagcc cccacaaccc aaatattaac gcgcgatcgc
    12781 ttttgccaaa aggaccgaca taactgcgaa caccgttaat cgtctgcgcg agtaaaccgc
    12841 aaaactcagt caatatggtg caaaataaca tgagtattac gagtgatgcg ttactttctg
    12901 gtaaaaataa aaacggtaaa tagagcgcaa tatcggaaat aacatcgcca gtttcattca
    12961 aaatcgcccc cagacgtgtt tgctgattgc actcacgcgc caacatgcca tccagcgcat
    13021 tgagtgccat acggataaaa agcacgatgg gcaatagcaa aaagaggatg ggttgtgcga
    13081 ccaacatcaa cagcaatccg gtaataagag aaagcgccag tgcagcaaga gtgatgtgat
    13141 tcgctgtaac atggtgctta taaagccaaa acatcgtcgg ccttaacagc gactgaaaga
    13201 gcggttttat ctggtagagc gtcattatca atccttgata attcggtttg cgctatggtg
    13261 catcaattcc cctgacggag atatcgcaac acgacgcttc attaaaaatt cagaatatat
    13321 caaggtattg gggactcact gtcaggaatg attatcccca ataaatcaag tggttaaaaa
    13381 ttattacgct gttattcaat acaactcatc aagttttctc atcgcttgtt cccgtattgc
    13441 gcgcgtctta ccgttaagcg ccagctggcg aactaaatcc agttcagggt taacaagctc
    13501 taacgccagc aaaggatcac gtttcagcct cgcctttaat acatcaactg gaaaacgggg
    13561 attattcatc gccacaatcc accactcggg atcaatttct gccgccagta ctgcaggcgg
    13621 tgtgtggcgg ttgttaagtg cgccgaactt gaaaaatgtg agattgcttt tcgccagtgt
    13681 actccaccat atacgatccg gttgttttgc cagtacatcc agttgtgcta acgcatcggg
    13741 gtctagtgca taataaacac gtaacgcttc gttaacctgc gcacgggaac tgttttcctg
    13801 cctcagaaaa aacttcgccc aaatatcatt gcgtgatgtg ttttgctgta attccttgag
    13861 aacaacagcg ggtaaattat cccaactaat taatgcactc tctaaaaagc cccagtcgtc
    13921 cgaacgtatc agagaggtgg catttaatag catctggtca tcaacttcac cgtagctgat
    13981 aagctcagac aaaatcatat cagcgctggt ttcatatgca tctgaatctg ctttgggatt
    14041 atttttccga tgcgataaag cacgttgcca aattttttgt ttcatcgacg tgctcagata
    14101 ctgatttttc gccagattac tccacccctc aggatcgttg aaacgtgtca ataaccgttg
    14161 taaagcagcg tcagagtgaa ttatctgcat atcccgacag caaaagtagg caggggattt
    14221 tttctccatc cttttaacaa aattaaactg taagttatct ggcaacgcta aaaataacgc
    14281 acctattaga tcatcgtcat tctcgaattt ttggtataaa tatactgcta ttttgtcact
    14341 ctcttgtggc gtcatccgca acaattgacc atggtgtttc atctctgcca gctttaacgc
    14401 taagcttgtg acaacatcgt ttgatgaatc gttcgccaac tgcattgcct gattgtaggt
    14461 gagttgtcct ccagcaccga cctgacgaat agaggaatca tcactgcgga tatagccatc
    14521 aataatggat tcgggcaata accacgcatc tttataaagc tcctgaatga tcatatccca
    14581 atctcttttc gccagagtca tgacgatatc cagagggaga ttttcacgta gcgcaagatt
    14641 caacggaccg acaccatcag gtagatgact cattcgcgtc attatttcga cagagagcgg
    14701 ttcacggtcg agcaacccca gaacaacagg ctggtaaccc aattgctgat cggtcaactc
    14761 cagaatctca ttgtcaggca tatatctcgc catacttgca atcgtttgtc catcattgct
    14821 attaataagt tgtttgcgta ggttagctga aattccttta agccttggga gagttcgcat
    14881 tatggcaaga tcaaatgtcg caatatctcg tccttcaaat gcagcaacgt tgccttcatc
    14941 cagcatttgc aattcctgca ctaactcagg agaaggctta gggcgaaaca gcgcttcttt
    15001 tttcatctga ttacagacat tccctgccat gcgaatattg cttcgcttaa aaatttcttt
    15061 cagagcatct tcatcattta ttagacccca ataatatgca tcaggattta ttatcttcaa
    15121 cgcttttttg acattttcag tgtcattttt atcactcgca cggaccatga attcactatt
    15181 tgtcgaagac gcaacaacag gtgcaattat gtcattgata aatatcccac gcgcattaat
    15241 actcccctta atcgtagacg gattatcgta atatgagtcg ataaatacca gtccacttgc
    15301 atttatcgaa ccggtaatta acagtggtga atccgtaact atattcgttg ccgtcacgtt
    15361 accgagaata acccatggat tagaaagaga gaaagtggaa atattacctt tgactgtcaa
    15421 attgccaaga acaatcaatt tttgattact ctctatatta ccttctacca ctaaatcgcc
    15481 acgatgcact ttccagtccg atgcagataa accgaagggg acaagattca cgctactgtt
    15541 gatagcttgt tcaacttgtt gtttatcttc agcggtagcc gcagaaaaaa atggttcaat
    15601 atcaactgca tttgcaacac atcgaagttc cgcctgtact ggaagactca gcataaacag
    15661 ggggagtaaa cctatccagc tgcaaatcct gggcttaaac atatgcactc catgccatta
    15721 aaaaagaaac aaaagggaaa tgcatctttc ccttttgtat attcgttatt ctcgcgaaat
    15781 accatccaac gtagacaaca cttcctcaga aagatgcaat ttttcagcag ccatattctc
    15841 ccgtaaatgc gcaacagaag acgtccctgg gatcagcaaa atattcggtg aacgttgtaa
    15901 cagccacgcc agcgccacct gcattggtgt tgcacccagg ctcgcagcaa catcggaaag
    15961 cgtggaagat tgcagcggcg taaagccccc gagcggaaag aacggcacgt aggcaatgcc
    16021 gtcacgagcc aaagcttcaa tcattgcgtc atcagcacga tgagcaatgt tgtattcgtt
    16081 ctgcacacag acgatttcgg caatcttgcg cgcctctgca acctgcgtcg gtgtgacgtt
    16141 gctcaggcca atatgtttta ccaggccttg ttgttgcatc tctgccagca cggtcaggct
    16201 ggcttcaatc gatccttccg ccgggccatg accatccccc atcatcacgc gcaggttaac
    16261 cacatccagt atgtccaggc cgagattacg tagattatcg tgtaccgctt tttgcagctc
    16321 tgccggagaa aatgcgggca accaggatgc gtcctctcca cgccgcgcac caattttagt
    16381 gacaattgtc agatcgtcag agtaaggata aagcgcttcg cggataatct gattggtgac
    16441 gtgcggacca taaaagtcgc tggtatcaat gtgattgacg cccaatgcca gcgcctcacg
    16501 cagcacggtt atagcgacgt ggcgatctct tggggggcca aaaaccccag gacctgccag
    16561 ttgcatcgcg ccataaccaa gacggttaac ggattttgta ccgagagtaa atgtattgct
    16621 gctcatcgca ccctgccctg taataaaacg gattaagaaa aatcagtata gaaacgaaaa
    16681 gagataaata atgcgaaggc gtaaacaaga tttctggatg agtagcacta ttgtcctgaa
    16741 agacaccaaa acattagaca gcaaaagata agtcaagcgt cgcatccggc aataaaaaac
    16801 cgcaacctgt atcagcgttg cggtttgtgt atcacatgct aaaaattaga acgttttctt
    16861 aaagttcagc atcacgcctt tgtcgctgat gccgtcctct ttccagtgat acgcatccag
    16921 attcagcgaa ctttctttgc tactgtactt cacgccgacg ctcgccaggc tgttgatacc
    16981 gccgcccttc tgaccgtcat cgacgttaaa gtgctggctg cctgcccctg caaggcttgc
    17041 cgtgcgctgg ctcttgctgt agctcaggtt cggcccgcct tccagcgtcg cattcgcgct
    17101 ccagccgcct ttccctgcgt agtccagttt cagcccgacg atgctgtcca ccgccgtttc
    17161 gctgccgctg ttcatgctca ggttaaagtc tccggcattg cgctcctgat agccgccttc
    17221 cagcgtgtga cgcagtttca ctccggcgta cggggtgatt ttcagccctt cgcgcggttc
    17281 aaaggttttt gccccttcgc tgcggaactc caggtactgc tgtttcacat ccgtgtctgc
    17341 cgttttgctg acgtcgccgt aagccaccga gcggctgctg tcgaggttat gcacgtcata
    17401 gcgcagggcg ttgttccagt tcatcccgtt gtcgaagaac atctggtgct tcaggccgaa
    17461 gaactggctg taaccaccgg ttacgccgtt gtcgcccgct ttctgcgcac cgtcgccatc
    17521 cagacgggcg ataccgtact ccagactcag gctctggctt tcgctcaggt caacggtttt
    17581 acgcaacgcc agcatgtcgt actcggtgtt atttccgagt tcagcacgcg ggtcgccttt
    17641 cgccaccacg ttaaacgcca gaccgttgcc catcttcggc gcggcgtccg ccagcatgct
    17701 gaagcggttg ctcagcacgc gcgcctcgcg gaataccgtg gttgcctggc tgccgctcac
    17761 ctgcttcagg gcgctgttca gttcggcagt agtgccgacg ttcaggctgg tatacagctc
    17821 gttgttggtg taacccgcat ccagtgcctt cgccacatcg ttcaccgaag catcggtcgc
    17881 cacatcggtg taggcgtttt tgctcatggt gacgtcaacg ttgccgctgg catcggtgct
    17941 gcctttggcg gtccacacca cggacgttga ggtgatggca tcggcgtcgg tcaggttgct
    18001 gccttccacc acgttgtcga aactgaccgt ggtgtctgcc gtaccggcgg cgaaaccggt
    18061 gttgatcccg acaccgttca tgctggcgtt gttgaccttg agcttaccgg cgctgccgtc
    18121 aacgttggta ccgaccacat aaccgttgag gttgttgcta ccgctgccag tcgcatctcc
    18181 ccctgttgtt acgccaccag aactggtgtt cggcacttcc ggcaacgtgt agtcggtgta
    18241 atgcacttcg gtattattac cgttgttgcc gttcacccct tccacgttca cgctgtcgcc
    18301 ctgcttaatc aacccggaac cggtcacgcc atcggcaatc accacggtac cgttgttaac
    18361 gatatggcct tgtgttccca acacactgaa tgcaaaggaa tcgttagcat agatattaat
    18421 cgtgccgttg ttctcgatca ccgcgtctgc ggtagcgttg gcatccagtt gcataccaat
    18481 taacccggta tcggtggtac cttcggtgcc gatattgata acgccgttgt taaccagttg
    18541 tgcgttttta tcaccttcca tcgccacggc attgttgccg ctgatgttca tcacgccatc
    18601 gttgagggca acatagttgt tgtgggtgaa tttaactgcg ctggcgttgt ctttatcgaa
    18661 gttaatcacc ccgccatcct ggttccacag gatggcatcg ctttctgtcg caccagcggt
    18721 acttaccgcc acagtgactt tcgcatccac cacgcccttg ttaataaact ggctggcgcc
    18781 gtcgtcatag tgaatagcgc ccgcgccaag tgcggcatcc agcgtcattt caccggtgtt
    18841 atataacgtg gcgttagcat tgcttccctg gaagcgaatg gcgtttttac tgttatcaag
    18901 gatgagcttg ccgctgttgt tgaacgttga ttggtcgtca atatccagcg tcccggcatt
    18961 gttattttca tcctgataac ccattttcca ggtccccgcg ttattcacca ccgcagagtt
    19021 atagagaatg gcacctttac gcgcctcgcc gcctgcaagg ttattgaata cagcatcgcc
    19081 ctgggcgtta acgcgcgccg acgcgatacc gctgttattc accgtgccat cgttaagctg
    19141 taaggttgac agtgaaccgg tattattaat taccgcgttg ctgtttttct caatttttac
    19201 gacgtcatta agcgttccgt tgttattgaa agtgcctcct tccagcgtca ccgtattcaa
    19261 tacaccttca ttggttaacg taccgcctgc attaacgacg atagccgaat tgatggttcc
    19321 gctcgactca ttaaccagcg cgccattttc aagaatctgg atttttccat tcttaacggt
    19381 acccgcgctg gtaacatccc cattaataat cgcaccagat gataatgata cagtctcacc
    19441 cgcagagctt aatacatcgc catcatcata caaatcggcg atatagttat ccgtcggatt
    19501 atactcatcg gccccctgac aaacgccaaa ggtacaaatg gtgccatagt tgataataag
    19561 cccagtgcca tcgttataga atgcctgacc attttcagca ttaatattaa taacaccggt
    19621 ttgatcattg atagccgttc ctttctcgta aacagccatg ccgacgagtt tatttacgcc
    19681 aagcgagtcg ttgtaatttt catttttctc aaggttgatc gttccctggt taatgaccgt
    19741 gttggtatta ccatacgccg ccatccccgc acccgcgttg tagacggtaa tcacaccatt
    19801 ctcgtggttg gtagacgtcg ctttggttaa tgctccatag taagcatcgg ttcccgaagc
    19861 cattcccaca ccgaaatctt tcgccgtgct atttgcgatg ttattacgca gcgatgtttc
    19921 atcattttca tcgacccaca gcgtatccag ggtgatttgc ccttcgttat ccgcggttga
    19981 tttatcgata gccaacatcc ccacgctgcc acgaccatac atctcaataa tgcccgtttc
    20041 gccgttcaga atggatgacg agtagctggc aagcatacct gtcaaaccta atgctctgtt
    20101 attgaaagag gtaccgtcag acaagttcca ctctgccgga taattcagca cgctgtgctg
    20161 attcatcatt ttggtggtca aggtgccatt gttaacgcca atccccccgt attcaacatc
    20221 gaagactttt ggtgccgggt cggtaccaaa atcgtacatc gccaggccaa tggatccgtt
    20281 attgatgacc gttgaatctt tattccaggc agccgcaaaa ccgatatttt tcagttcaac
    20341 ttcaccgtta ttggtcgcct tcgcaccaga atcaatacta atggtggata acgccacgcg
    20401 ataataatag gtgaaggaat ccacatcact catatcaaac gtcgcgtcag cgccaatctc
    20461 cagctccgcg ccatgactca actgaatgag attttcgaaa cccaacggga actcgccgct
    20521 aatggttgac tcaccattga ggtagaccga tgaatgccca tcaacgctaa taagcggtgt
    20581 gtctccgata ccacgacgag aagcagatgg ttttgagcca tcatccatga catttttttc
    20641 gccgacaaaa tggacaccgc cattaagcgt aaaggtatta tcatcgccag caataacaat
    20701 acctgcaatg tgctcctgag cgccgtcgta acttgcatag gaacgcaccg tggtttcgct
    20761 atcagcaaca acggttaact gaccatccag cgtgacgtca ttgttattac cgctgatatt
    20821 tacgccggtt gagggattaa agaagtaagg ttgcgcatta tcggctttct gatctttatc
    20881 aaccagaatg ctgcctgcga tttcaatcct attgccatca cccgaaacat taacccccgt
    20941 cgctttttgc cctgtcacat tcagctcata agtcgccagc gtcatgttgt tattattgcc
    21001 agagacatca agaccggtgg agaaatcccc aacgtctaac ccacccgcga gtgaaatatt
    21061 agctttatcc ccagcaacgc tgacaccggt tccggcattg gtcgcggaaa tatcaccaac
    21121 gttaacaaat gtcgcattat caccgtcgat ctgaatgccg attgcatttt cacccgcagc
    21181 ggcctgacca acattgaggt cgcccatgtt gatcacgctg acgccatcac catcaagcag
    21241 catgccaata gagttatctt cgatgaccgt aatattacct ttgttgctaa cgaacgtatc
    21301 attgccgtca acgtcaatac cgtgcgcacc accgcttacg tataagtcgc cttcctgttt
    21361 gatggtggca ccgtcaccga caattttcga gccagtggaa ttctcaccgt caacgctcat
    21421 ggtaccttga ttatcgaccc gcgcattatc gccattgata aggctacccg taccaccatt
    21481 ggtgatggtc gagtcgcccg cagtgttcag cgtagcatta tcgccattga tgattactgc
    21541 ggtaccgccg tcaacttccg tctcgccgtt gttattgata gtgatatcgt caccagtaag
    21601 gataccaacg accgaaccct cgccgatggc ggtcgtttta ccgtcgttgt tgagggtgat
    21661 gttgtcaccc tcaaccgtca tgacagtacc gccattcagc gccgtattgc cgccctgatc
    21721 ggtgatgatc gcattaccaa aatcaatcgc agttccggtt gtgccatcaa caacgacacc
    21781 ggcctcttta gtgatatgga tgagcccatc atcgtcgtag ctccaggtga tgccacctgc
    21841 cgtatttacg ccatcaaaga tgacggtgtt gctgtcgtca tggactgtcc agcccttgac
    21901 tatcgtcgtc aggccatcag gcgcggttaa ggtgtaactc ccatcggcat tttggctgta
    21961 ggtaaaggtg gtttcgcgaa ttttgacggt tttcgcgtcc tgatcccagg ttacgtcatt
    22021 tttatagacg acaggatcgt gtttaaccgg ggtatcgcca ccatcgtctg gcggaacagg
    22081 cgtatcaccg ccgtcgtctg gaactggcgt atcaccgcca tcatctggtg gaaccggcgt
    22141 atcaccgccg tcatctggtg gaaccggcgt atcaccgccg tcatctggcg gaactggcgt
    22201 atcaccgccg tcatctggcg gaactggcgt atcaccgccg tcatctggcg gaaccggcgt
    22261 atcatcgccg tcatctggcg gaactggcgt atcaccgccg tcatctggcg gaactggcgt
    22321 atcaccgccg tcatctggcg gaaccggcgt atcaccgccg tcatctggcg gaaccggcgt
    22381 atcaccgccg tcgtctggcg gaacaggcgt atcaccgcca tcatccggaa ctggggagtt
    22441 attatggtgg tgattgtcat cgtcacttaa ggtatatata gcaattcccg tagctattgc
    22501 agcaagacct gcagctgccc atggtaaaac tgcactatct ttattttctc tgcactccgc
    22561 ggataaattt tctagctgct ctttgctcag ggaatgaata tttgccggac atgttatctt
    22621 actggactta cgtgtttttt cggttgagtc gggtattgaa gtatccgcag cccatgtctg
    22681 gctacttaac gccatggcaa cacaaacaga caataatttt ttcctttgca tataaaattc
    22741 cttagatcta gaccgtgcgt aacaagccat gcagaatttg aagataaatc gtagtaaata
    22801 ttcatagtga
//
LOCUS       DAZGBC010000075        22806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGBC010000075 DAZGBC010000000
VERSION     DAZGBC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.075"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..1031
                     /locus_tag="ACHK1J_002760"
     CDS             9..1031
                     /locus_tag="ACHK1J_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO7912004.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSQ
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1031..2011
                     /locus_tag="ACHK1J_002761"
     CDS             1031..2011
                     /locus_tag="ACHK1J_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO7912005.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2008..2766
                     /locus_tag="ACHK1J_002762"
     CDS             2008..2766
                     /locus_tag="ACHK1J_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7912006.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2776..3588
                     /locus_tag="ACHK1J_002763"
     CDS             2776..3588
                     /locus_tag="ACHK1J_002763"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7912007.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLISKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            3585..4439
                     /gene="modD"
                     /locus_tag="ACHK1J_002764"
     CDS             3585..4439
                     /gene="modD"
                     /locus_tag="ACHK1J_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO7912008.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4465..6435
                     /locus_tag="ACHK1J_002765"
     CDS             4465..6435
                     /locus_tag="ACHK1J_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO7912009.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAVQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINVTTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6485..6739)
                     /locus_tag="ACHK1J_002766"
     CDS             complement(6485..6739)
                     /locus_tag="ACHK1J_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7912010.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6940..7674
                     /locus_tag="ACHK1J_002767"
     CDS             6940..7674
                     /locus_tag="ACHK1J_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO7912011.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKARHITITAETQGAKVEFTVEQLQQSEYLQLPAFIS
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7676..8287)
                     /gene="emtA"
                     /locus_tag="ACHK1J_002768"
     CDS             complement(7676..8287)
                     /gene="emtA"
                     /locus_tag="ACHK1J_002768"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO7912012.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8387..9301
                     /gene="ldcA"
                     /locus_tag="ACHK1J_002769"
     CDS             8387..9301
                     /gene="ldcA"
                     /locus_tag="ACHK1J_002769"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO7912013.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPICRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAMLNNTHEGTQLTISGHPVLKM"
     gene            9396..11132
                     /locus_tag="ACHK1J_002770"
     CDS             9396..11132
                     /locus_tag="ACHK1J_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO7912014.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11253..11414
                     /locus_tag="ACHK1J_002771"
     CDS             11253..11414
                     /locus_tag="ACHK1J_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912015.1"
                     /translation="MDDKPLQALANKLKTPAQESQRNGFFRLQQVKNNQPRITGIPVN
                     HQGPQPDIA"
     gene            11753..13189
                     /locus_tag="ACHK1J_002772"
     CDS             11753..13189
                     /locus_tag="ACHK1J_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912016.1"
                     /translation="MASFKDICPFAPAIPPSSVNTGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIVDLSIAGKVNIQVDNNTIITLGTPLNQQHNEFMMVAKWCEW
                     AIQQDGLQENLQKKLHEVLEENRQNKQSEIPQEDLKERLKEIKEDILKENQSASQIED
                     RAEALRRMKECLITRQSMLDLSNLGLTSLPENLPPHLIEFNCSRNVLTALPEVMPKGL
                     RVLECMENFLILLPKVQPPKLMVLKCYENYIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISSPRLPPKLIIYYCGENQF
                     KTVRVPQPQSLKVFDCNGNPWDKDNLPTLLKAVEGLKNQQGLEDLLDFLRKEGQIDLE
                     GLEELEDLDDLMDLEFLDDPELLERVKVQEDLELLNQQLGLLSLERQQDSQPVNQQSE
                     HEPEPASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(13358..14428)
                     /gene="dadX"
                     /locus_tag="ACHK1J_002773"
     CDS             complement(13358..14428)
                     /gene="dadX"
                     /locus_tag="ACHK1J_002773"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO7912017.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14438..15736)
                     /gene="dadA"
                     /locus_tag="ACHK1J_002774"
     CDS             complement(14438..15736)
                     /gene="dadA"
                     /locus_tag="ACHK1J_002774"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO7912018.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16066..17598
                     /locus_tag="ACHK1J_002775"
     CDS             16066..17598
                     /locus_tag="ACHK1J_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO7912019.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHHLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(17650..18369)
                     /gene="fadR"
                     /locus_tag="ACHK1J_002776"
     CDS             complement(17650..18369)
                     /gene="fadR"
                     /locus_tag="ACHK1J_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO7912020.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18591..20132
                     /gene="nhaB"
                     /locus_tag="ACHK1J_002777"
     CDS             18591..20132
                     /gene="nhaB"
                     /locus_tag="ACHK1J_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO7912021.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20278..20808
                     /gene="dsbB"
                     /locus_tag="ACHK1J_002778"
     CDS             20278..20808
                     /gene="dsbB"
                     /locus_tag="ACHK1J_002778"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO7912022.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20854..22122)
                     /gene="umuC"
                     /locus_tag="ACHK1J_002779"
     CDS             complement(20854..22122)
                     /gene="umuC"
                     /locus_tag="ACHK1J_002779"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO7912023.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22122..22541)
                     /locus_tag="ACHK1J_002780"
     CDS             complement(22122..22541)
                     /locus_tag="ACHK1J_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7912024.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5437 a         5978 c         5752 g         5639 t
ORIGIN      
        1 tataacttat gtcactaacc agacggaggt ttacacagat tcttgcgtcg acgttgttcc
       61 tgcatcatct gccttctttt gcacaatcag tcaaattctg ggcctcactg acgcttcccg
      121 aagcccaaaa cattacccgc atcgtcagcg caggtgcgcc cgccgatttg ctattgctgg
      181 ctgtcgcgcc agaaaaaatg gtcggttttt cctcttttga ttttgcccgt caggcattaa
      241 ttccattgcc agagcacatt cgccagttac ccaggctggg acgactcgcc gggcgcgcca
      301 gcacactctc gcaggaaggg cttatggcgt tacatcccga tttggtcgtt gattgcggca
      361 atacggatga aacctggatc tcccaggcac ggcaggttag cgaacagaca caaataccct
      421 ggttattgct taacgggaaa ctggagcaat cagcagaaca gttaacaacg cttggcaaaa
      481 cgttaggcga agagcatcgc gccgcagaac aagccaatct cgccagccgc ttcgttggtg
      541 aagctcaggc attcgccacc tcacccgccg ctaacctccg cttttatgct gcgcgcggtc
      601 ctcgagggct ggaaacgggc ttacagggtt cgttgcatac cgaggcggcg gaattattag
      661 gtttgcacaa cgtcgcgcaa atagccgatc gccacggcct gacacaggtt tccatggaaa
      721 atctcctgcg ctggcagccg gatattattc tggttcagga ggccgttact gcagacttta
      781 ttcgtcgtga tccactctgg cagggcgtga aagccgttgc ggaacaacgc atcttatttt
      841 taagtggctt gccctttggc tggctggatg ccccaccggg aatcaaccgt cttctgggat
      901 tacgcagact tcacgcctgg ctggatcccg ccatcaatcg ccagtttaaa agtgacatgc
      961 agcattacgc ccaactgttc tggcattgtt cactcagtga cgccgactat caaaaattgg
     1021 tggcgagcta atgagaatcg taaacggatg tattttgctg ctggcagcta tcagtatcac
     1081 ttttgccgcc gtaagtgggg cttatcatct cgatatgcag caactgctgg cgctaattct
     1141 tcgtcaagaa aatgttcctg tgcaggaaca aattgtcttc tggcaaatcc gcgtgccgcg
     1201 tattcttgct gcgctgttac tgggcgcggc gctggcagga gccggaacca cctatcaggg
     1261 aatgttgcgt aatcccttag tttcaccgga tattttaggc gtctccgccg gtgccggact
     1321 tggtgcctgc acggcaattc tgtggggatt atccattgtt tacatccagc tgtacgcatt
     1381 ttgcggcggg ctgatggtgg tcgcgggcgt ctggttgatt acgcgtcggg tgacccgcca
     1441 cgatccgatc ttaaccctgg tgctggtagg cattgcgtta ggaacgttgt gcggagcggg
     1501 gatatcgcta attaaaacgc tggccgatcc ttacacccag ttaccgtcaa ttaccttctg
     1561 gttgctgggg ggactgtcca ccgtgacctt acgcgatctc tgctatgctg caccgatcat
     1621 tctcataggc tccttgcccc tgttttttct ccgctggcgc atgaatttac tcacgctttc
     1681 cgacgacgaa gcccgttcgc tgggactgaa cgtgacgcgt ctgcgctttg gattgattgt
     1741 ctgcgccaca ctaatcaccg ccagtacggt agcaatagcg ggcataattg gttgggtcgg
     1801 cctggtcgtt ccgcacattg cccgcttgtt gaccggtcat aaccatcagc agctgttgcc
     1861 catggcaatg tgtactggcg cgattctgct cctgcttacc gatacgctgg cacgcagcat
     1921 tggaacaaca gaaatcccac ttggcattct gaccgctttt gttggcgcac cgtttttcct
     1981 gcttttgttg ttacgcggag gtcgccagtg actttgcttg ccgtccgtca tgcgtctctg
     2041 ggttactccc gccatccggt gctacgcgac gtgtccttca ccctgtcgca aggaacaatt
     2101 tgttgtctgt taggagccaa tggttgcggc aaaaccacac tcatgcgctc cattcttggc
     2161 gttataccgt tattaaaagg cgaggtctta ctggatagcc tccctgtgca gacactcagc
     2221 catcgccagc gcgcgcaggc tattgcctgg gtgccgcagg cgcacgacgg tatttttgct
     2281 tttagtgtgc tggatatggt gttgatgggc ctggccccca ccataggcgc tttttccgtt
     2341 cccggtaagc aagaacgcct gaaagccacg gagcaactgg aaaagctggg cattttgcat
     2401 ctggcggcac gacgctggaa tacgcttagc ggcggtgaac gtcagctggc tttaatcgcc
     2461 cgtgcactcg tccagcaacc gcgcttgttg ttactggatg agcctgcctc cagcctcgat
     2521 ttcggtcatc agatccaact actggacacg ctggcacagc tcaaaaataa cggcatgaca
     2581 atgttaatgt cgactcatca tccgctccat gccaacgcca ttgccgacag cattatccag
     2641 gtagagccag atggacgagt aacacaaggc ttaccgaccg aacagttaac aacaaacaaa
     2701 ctggctgccc tgtatcgggt ttctgccgac cagattcatc atcacctctc tgcaattagc
     2761 cactaaggaa caatcatgct cattgatgat attgattttg cagacctcta cctgcagcaa
     2821 ctcaaactgg cccaccgcac agaaaaaacg ccggatcact gggatcaacg ggcggaaaaa
     2881 atggcggaaa attgcgcctc gcctacggac agctacctgc aacaactgat ctcaaaaatt
     2941 gacctacaag gcgctcagac cttattcgat atgggctgcg gaccgggtac cgtatcgctg
     3001 gcgctggctg acaaactcac gacgatttat ggcgttgatt acagtcaggg gatgctgaac
     3061 gttgccgccc gtcgcgccgc cgcgctgaag gcagacaatg tccactggat ccagcgcgcc
     3121 tgggaagagg actggagtga cctgccacgc tgcgatattg ctgtggcttc ccgctccact
     3181 ctggtggctg atatgcgcca ggcaatgagc aaactgaata atcaggcacg gcttcgcgtc
     3241 tatacgactc atctggtcag cacctcgttc gtctctccag ccattcagcg ggcggctggt
     3301 cgggaagtga tcgaattacc caactatatc tttgcgctca acgtcctgta tcagatggga
     3361 atttatgccc acgttgattt tatccgtgga caaaattgcc aacaggataa cagcacctgg
     3421 gaacgttttg agcagaacgt cagctggagt cttggcgcgc ttaacgacga tgagcgtgag
     3481 cggttatatc gctggtatca acaacaagat gctcgtgccc tcgcccctgc cagccgcgac
     3541 tgggcgctca tctggtggga tagcgtaccg caggaggcac tgcgatgatt ttcctttctc
     3601 aggcacaaat cgatgcgtta cttctggaag atatccaggg cggtgacctg accacgagag
     3661 cattaaatat tggacaccag catggctata tagagttttt tctccgtcag ggcggctgcg
     3721 tcagcggcgt ttctgtcgcg tgtaagatgt taactacgct gggattaact attgatgacg
     3781 cggtcagcga cggttcacaa gcgaacgcgg gtcagcggct aatccgtgcg cagggtaatg
     3841 ccgcagcact tcatcaggga tggaaggcaa tccagaatgt gctggagtgg agttgcggtg
     3901 tttctgatta tctcgatcaa atgctggcgt tacttcgtga acgttatcct gatggcaata
     3961 tcgcctgcac ccgaaaagca attccgggca cccgcttact ggcctcgcag gcaattctgg
     4021 ctgccggagg actgattcat cgtgccggat gtgcggaaac catattattg tttgctaacc
     4081 accgccattt tcttcatgac aatcaggact ggtcaggcgc aatcaatcag ttacgtcgcc
     4141 acgcacccga gaagaaaatt gttgttgaag ccgacacgcc gaaagaggca atcgccgcgt
     4201 tacgcgcgca accagacgta cttcagctcg acaaatttag tccgcaacag gcaactgaaa
     4261 ttgcgcaaat agcgccgtcg ctggctcccc actgcacgct ggctctgacc ggcggaatta
     4321 atctgacgac actcaaaaat tacctcgact gcggcattcg cctctttatt acctccgcgc
     4381 cttattacgc ggcaccggct gacatcaaag ttagtctgca acccgcagcc tctatttaat
     4441 tctttgttaa acggaattta ttctatgaga ctcaaaaaac attatctctg cacagcatta
     4501 tcacttgcct ttacccagca ggccgtagcg gttcaggaga gcgactcgct gaccgtatgg
     4561 tccagtccgg tatcatcgac gacgaccacc gttctcgatc aacccaccat gaaggccctg
     4621 gataaacaga atgtcgctca ggcattaagt gtcgtccccg gcgtggtgct gcaaaagtca
     4681 ggcagccgca acgaagaaca ggtaaaagtt cgtggctttg atagtcggca ggtgccggtc
     4741 tatttcgacg gtgtgcccat ttatgttccc tatgacggca acctcgatct ggcgcggatt
     4801 ctgaccaaca atctgggggc agtcgaagtt tccaaagggt attcgtcgct gcttcagggg
     4861 cctaatcaga tgggcggagc cattaatgtc accacccaga agccaacgaa acctctggaa
     4921 gcaagtctgg gatatcgcca gggatggagc cgtagccagg acaatgccta cgatatgcat
     4981 gcttcatttg ccgccagcag cgaactgggg tatttgcaag tcagcggtag tcagctaaag
     5041 caggattttc tcggcctgcc gcatggtgta aataatgata ttgcaggcaa acacggcaag
     5101 atgattaatt catcggctga tgataaacgc ggcattgtga agctcggatt tacaccacgt
     5161 gaaaacgatg aatacacact gacttacatt aagcaggatg gtgaaaaaga taacccgcca
     5221 tacagcggaa atagtggtca aaaatcacgc tactggcagt ggccagagta tgacaaagaa
     5281 agtttttatt atcagggaac gacccaacta aacgatcgtt ttaccctgaa aagtcggctg
     5341 tatcgcgaca cctttgaaaa tacgctgatg atgtacaact cgctggctga tttgaaaaat
     5401 aaaaaaggca gctacagcca ttattccgac tacagcgacg gtgccggatt acaactggca
     5461 gccgatgtgc gtgaaaacga tctgctgtcg tttgccgtta actggaaaga tgacgtacac
     5521 cgggaaaaag gtgcgccgca cgccgcttac gatcgctatg aagatcgtac ctggtcgctc
     5581 gccagtgaat atcaatgggc tgctgccgat aatgtcgatg ttgtggctgg aatcagctat
     5641 gactggcgcg atagtgtaga agcgaaaaaa cacgagaaag atggcagcat cacccattat
     5701 gacgacaaca atcagtcagc ttttaactgg caggtgatgg ggaaatacca ctttgtcaat
     5761 gaagacacgc tggcgctttc gtactatgac cgcacacgct ttccgacgct gaaagaacgc
     5821 tataccacgt ccaaacctgc gtataaccag atagcgattg ttaacccgca gctcaagccg
     5881 gaacgcgctc gcggggtgga tttaacctgg aatggtgcct tcacgcgcga ctggggattt
     5941 gaggtcagcg tttactataa ccgggtgagt gatgccatcc tctcgcacaa tatcgatgcc
     6001 gataccattc aaaatcagaa cagcggcacg gtggattaca gcggtctgga tgccggtatt
     6061 aaggggaaaa tcagcaatat actggatgta ggattgagct acgccctgat ccacgctgac
     6121 gccaaacgca aagacatcgg caagataacc gatctgccaa cgcagacaat gaccgcatgg
     6181 atgactctca aaccgtggga gccgttaagc gtaacgctgt cggaagaggc gcgttcctcc
     6241 agctacagca acagtgacgg ttcacaaaaa gccgccggtt ttgcggtgac ccacattcga
     6301 gccgattaca ccttaggtca tggcttcagc gtcaatgcgt cggtcaataa cctgtttgat
     6361 acccaatatg cctacagtga agggtttatt gaagaaggcc gcaatttctg ggcaggtgtc
     6421 gagtatacgt tctgatgtga aagcggatag cggcatctgt atcgctatcc gccttatgaa
     6481 aatcttattc tcttcgcagg aggcggaata cgcccagaac gagaatagct cccaccaccg
     6541 ccaccaggaa gctgtgcaaa ttaaaaccgc tgatggagcc gccaatgcca aacatggtcg
     6601 ccagccagcc gccgaccacc gcaccgacta tcccgagaat acaggtcagg aaaaatccac
     6661 caccatcacg ccccggcatg attagcttgg cgataatgcc ggctatcagg ccaaaaataa
     6721 tccaggcaat aattcccatg ttcaggccct cgtttcgatg aatcggacgc aagaaatgct
     6781 tacgtcaacg ctctaagtat aggcaagggc gggatcgatg ctgggcgttt gtcggctgat
     6841 ttaagaacaa catgtaaaaa atattcacgc atttgtatta agttttgtta actatgaccg
     6901 ataaaccaaa gacagtttgt cagtcaggag tttttccgcg tgagtcatta ccatgagcag
     6961 ttcctgaaac aaaatccgtt agccgtcctg ggcgtgttac gcgatttgca caaagccgca
     7021 attcctttgc gtatcagctg gaatggcggg cagctgatca gcaaaatact ggtaataacc
     7081 ccggataaac tggtgctgga tttcggcagt caagccgaag acaacatcgc cgtactaaag
     7141 gcacggcaca ttaccattac cgccgaaact cagggtgcga aagtcgagtt tactgttgaa
     7201 caactacaac agagtgaata cttgcagctt ccggcattta ttagcgtacc gcctcccacc
     7261 ttatggtttg tacaacgacg ccgatatttc cgcatctccg ccccactcca tccgccttat
     7321 ttttgccaga ccaaactggc ggataacagt acgttacgtt tccgcctgta tgatttgtcg
     7381 ttaggcggca tgggcgcatt actggaaaca gcaaagcctg ccgggttgca cgaaggtatg
     7441 cgcttcgctc agattgaagt caacatgggg caatggggtg tttttcactt tgacgcccag
     7501 ttaatctcca tcagcgagcg caaagtgatt gatggcaaga atgaaaccat caccactccc
     7561 cgtctgagct tccgttttct taacgtcagc ccgacggtgg agcggcaatt acagcggatt
     7621 attttctctc tcgagcggga agcccgggag aaagcggaca aagtacgcga ctgatttaca
     7681 tcgcgtccag tgcctgctca agtttgtaga tatagcgcgg agcctgcggc gcaggatgat
     7741 ttcgcgctac gtgatcgagg aactcgtcag catccagatc gttgattttg ctgattgcct
     7801 ttttccgatc tgacgagaaa gtccgtagca gcgcacctgc cccgttagcg tatgacacca
     7861 ccagcgcata ttgcagtacc ttcggatctt caatgcctgc cagcgggccg gtttccagaa
     7921 tattcaggta agccgccccc attgaaatat tacgctcagg atttttcagc tcgctggtcg
     7981 tcggctcacc actccagccc atacggcgat atacatcacg tccggaggtt gaagctttta
     8041 actgcatcaa accaatggca ttcgatttac tcaccgcgtt cggattacca cccgattcga
     8101 tagcgataat cgccgtgatc aattgtggat cgacgcccca ggctgcaccg gctttctggc
     8161 ttattggcat ccactgcatc gcacgttgca ccggaacttt cgcgttccac ggcgggttcg
     8221 tgtagtcatg ctttgatgaa caacccgcta ataacacaat caaaaaggca aaccatctca
     8281 atttcacttc ttctatcctt atagcctgga atcgccgttg aggtgacaac ggcacaccta
     8341 agcggcatga tatacatttc gtttctgttg tcagcaagga attgccatgt ctctgtttca
     8401 cttaattgcc ccatcgggtt actgcattaa acagcacgcc gcgctgcgtg gtatccagcg
     8461 ccttaccgac gcggggcatc aagtcaataa cgtcgaggtt attgcccgtc gttgtgagcg
     8521 ttttgcgggt acggaaacgg agcgtctgga agatctcaat tcccttgcca gactgaccac
     8581 ccccaacacc atcgtgctgg ctgtacgcgg cggttacggt gccagtcgtt tactggcaga
     8641 tattgactgg caggcactgg tggctcgcca acaatatgac ccgttgctca tatgcgggca
     8701 tagcgatttt accgccattc agtgcggtct tctggcgcaa ggcaatgtca tcacctttag
     8761 cggccccatg ctggtagcaa actttggcgc ggatgaactg aacaccttta ccgagcacca
     8821 tttctggctg gcgttacgca atgaaacctt taccattgaa tggcaagggg aaggcccgat
     8881 atgtcgggcc gaaggcacgt tgtggggagg caatcttgcg atgctgattt cactgatcgg
     8941 tacaccgtgg atgccaaaaa ttgagaacgg tattctggtg ctggaagata ttaacgagca
     9001 ccctttccgc gtcgaacgta tgctgttgca gctctatcat gctggaattt tgccacgtca
     9061 gaaggcgatt attctcggta gctttagcgg cagtacgcct aatgattatg acgcaggtta
     9121 caacctggag tcagtgtacg cgtttctgcg ttcccgcctg tcgattccac tcattaccgg
     9181 cctcgatttt ggtcatgaac aacgcacggt tacgctgccg ttaggcgcac atgccatgct
     9241 taataacacc catgaaggca ctcagttgac gattagcggt catcctgttc ttaaaatgta
     9301 aaaaaatcac gcttcagggc taagtaaaac gctgtctctg ccgctaatat ggtagggttt
     9361 atataagaga cagcgtaatc aggagtaacc gaccgttgga tgccacaaca ataattagcc
     9421 ttttcatctt aggatccatc cttgtcacca gcagtatatt acttagttca ttttcttccc
     9481 gtcttggcat tcctattctg gttatttttc tggcgatcgg catgctggcg ggagtcgatg
     9541 gcgtcggcgg tatcccgttt gataattacc ccttcgccta catggtaagt aacctggcgc
     9601 tggcgattat tttgctcgac ggcgggatgc gcactcaggc cagttccttt cgtgtggcgt
     9661 taggaccggc gctgtcgctg gcgacgctgg gcgtacttat cacctctggt ttaaccggca
     9721 tgatggcggc gtggctgttt aatcttgatt tgattgaagg cttattaatc ggcgctatcg
     9781 tcggctccac cgatgctgca gcggtctttt ctctgctggg tggtaagggg cttaacgaac
     9841 gtgttggctc gacgctggaa attgaatccg gcagtaatga tccaatggcg gtctttctga
     9901 cgattaccct gattgcgatg atccagcagc atgaaagcag tgtcagctgg atgttcgtgg
     9961 ttgatattct gcagcaattt ggcctcggga ttgtcatcgg tcttggcggt ggttatttac
    10021 tgctgcaaat gattaatcgc atcgccctgc ccgccggttt atatccattg ctggcgttaa
    10081 gcggcggtat tttaattttt gctttaacca cggcgctgga aggtagcggt attctggctg
    10141 tttatctgtg cggttttctg ctgggtaatc ggccgattcg caaccgctac ggcatcctgc
    10201 aaaatttcga cggcctcgcc tggctggcgc aaatcgccat gttcctggtg ctggggctgt
    10261 tggttaaccc aagcgatctg ctgcctattg ccattccggc gctcattttg tccgcatgga
    10321 tgattttctt cgcccgtcct ctttcggtat ttgccggatt gctaccattc cgtggcttca
    10381 atctgcgtga gcgcgtgttt atcagctggg tgggattacg cggcgcggtg ccgattattc
    10441 tggccgtgtt cccgatgatg gcggggctgg agaatgcacg tctgttcttt aatgtcgcct
    10501 tctttgtggt tctggtttca ctgctcttgc agggaacatc actctcgtgg gcggcgaaaa
    10561 aagccaaagt ggtggttccg cccgtcggac gtccggtgtc acgcgttggc ctggatattc
    10621 atccggaaaa tccgtgggag cagtttgttt atcaattgag tgccgataaa tggtgcgtgg
    10681 gcgcggcact gcgcgatttg catatgccaa aagagacgcg tattgcagcc ctgtttcgtg
    10741 ataaccagtt gcttcatcct accggcagca cccgactgcg cgaaggcgat gtattgtgtg
    10801 taattggtcg ggaacgcgat ctcccggcgc tcggtaaact gttcagccag tcgccaccgg
    10861 tcgcgctgga tcaacgcttc tttggtgact tcattctcga agccagcgcc aaatatgctg
    10921 atgttgcgct gatatatggc ctggaagacg ggcgagaata tcgtgataaa cagcaaacgc
    10981 tgggtgaaat tgtccagcag ttgttaggcg cggcaccggt tgtcggtgac caggtagagt
    11041 ttgccggaat gatctggacg gtggccgaga aagaagataa tgaagtgttg aagattggcg
    11101 ttcgagtggc tgaagaggaa gctgaatctt aaatttaatg aagcatctat cgccggttac
    11161 gatgctttgc ttaacgcagc ctgagcctgt atataaactt gtcatttcct gattttgata
    11221 tgcacattcc aacatcagaa gaaggttaaa ttatggatga caaaccattg caggctctgg
    11281 ccaataaact caaaacaccg gctcaagaaa gtcagcgtaa tggcttcttc cgactgcaac
    11341 aggtgaagaa taaccagccc cgcattaccg gtatccccgt caaccatcag gggccccagc
    11401 ctgacatcgc ctgaacatat tcccccacgc aacctgccgc actgacatag cccacacaat
    11461 aggcgtttca tccgcctgta gcaccggatg catgagcgat ttcttatagt tattcctttg
    11521 cttaataacg attatttccg ggtcgttcgc tctcgtcttt tctttaatac gtcagagcta
    11581 cctgggccgt aatacagcat gtaccagacc cgacacggca tttctgacca gggaaaaagt
    11641 caacgatgag tagttatatc agcgcaaatg acgtcctgtg gctcctttgc ggtgaccaca
    11701 ttcacgtctc cggcgctgcc agaggatatt tatttcaagg atacgtagca ttatggctag
    11761 ctttaaagac atctgcccat tcgcccccgc cattccccca tcttccgtta acacaggcgt
    11821 acgacgcccg ccagggcggc tcttcctgtc ggtggaactc aagaatcaaa ttttgcagaa
    11881 catgaagtat ctcagcccgc aaaaattcag ctggggtgat gccccctggc agattgtcga
    11941 tctcagtatc gcgggcaaag tgaacataca ggtggataac aataccatca tcacgttggg
    12001 gactcccctg aatcagcaac ataacgagtt catgatggtc gccaaatggt gcgaatgggc
    12061 tatacagcag gatggtttgc aagaaaacct gcaaaaaaaa ctgcacgagg tcctggaaga
    12121 aaaccggcaa aacaaacagt cagaaattcc ccaagaagac ctgaaagaaa ggctgaaaga
    12181 aatcaaggaa gatatcctga aagaaaacca gtcagcaagc cagattgaag accgggcaga
    12241 agcattgcgc agaatgaagg aatgtctcat aacaagacag agtatgctcg atcttagcaa
    12301 ccttggactg acttcactcc ctgaaaattt gcctccacat ctgattgaat ttaactgcag
    12361 tagaaacgtg ttgaccgcgt taccggaggt aatgccaaag gggctgagag tgcttgaatg
    12421 tatggagaac tttttgatct tgttaccgaa ggtgcagccc ccgaaactga tggtactgaa
    12481 gtgctatgaa aactatatta tctggctgcc tgagctgtcg actaacctga gagtgattaa
    12541 ctgttctgaa aacttcttgc aatttttacc gccgtcgatg ccccagtacc tgtataaact
    12601 gtcctgtgct ggcaacaata ttaactcaat acctgatgag atgctggaga acttgactcg
    12661 cctgaaggta ttcgactgtt ctagtaacga tttgatctct tcaccacggc tgccgcccaa
    12721 actaatcata tactactgtg gagaaaatca gtttaaaact gtacgggtgc cgcagcccca
    12781 gagcctgaag gtgtttgact gtaatggtaa cccttgggac aaagacaatt taccgacgct
    12841 gctcaaagcc gtcgagggcc tgaaaaacca gcagggcctg gaggaccttt tagacttttt
    12901 gcgcaaggaa ggccagattg acctggaggg actggaggag ctggaggacc tggatgacct
    12961 tatggatctg gagttcctgg atgacccgga actcctggag cgcgtgaagg tacaggagga
    13021 cctggaactc ctgaatcagc agttgggcct gttgagtctg gaaagacaac aggactcgca
    13081 gcctgttaat caacaatctg aacatgaacc cgaacctgca tcaagggtga agcgtgattt
    13141 atctgaagtc gagttcgagt caacaatgaa acgtaagcgt tttatgtaga acgcctgtgc
    13201 ggtgagttca ggttccgggg aaatagtgtc catcatttta atgggcacta tcatgggcaa
    13261 tggctctgtc acattaacaa ctacagttgc tgaccagccg gatggatgat tttttttgca
    13321 accagaagac gttgcctccg atccggctta caacaagtta caccgtcaca accgggacac
    13381 gtaacgccag cgcgcacatc aactcatagc ccaccgttcc ggcagcggcg gcgacatcat
    13441 caattttgat ctccttgccc cacagctcaa ccggcgtacc aatacccgcc tgcgggcaag
    13501 gcgttaaatc gaccgccagc atatccatcg aaactgtccc tactgtcatg gtgcgcacgc
    13561 cgtcaactaa aacaggggtg ccggtaggcg catggcgcgg ataaccgtcg gcgtatcctg
    13621 cggcgacaat gccgattcgc tgttcatctc gcgcagtata gcgaccgccg tagcccacac
    13681 gctcgcccgc ttttagcgtc tggacaccaa taatctcact gcttagcgtc atcaccggac
    13741 gtaatccggt attggcgata tcacgccact gaccggacgg cgaagcgcca tacaaaataa
    13801 tgccaggccg aacccagtca aaatgcgctt ccgggtgcca cagagtcgcc gccgaattgg
    13861 acaacgaacg ccgacactcc agcccttccg ccgcctgctc aatacgcgcc atcgcgccgg
    13921 aaattccatc aggatgttcc gcctcggcaa aatgcgacat cagggtcatt tcgccaacat
    13981 tcgccattgc ccgcaactgc tgccagacgg taagcacgcg atcgggctgg aagcccaacc
    14041 gattcatccc actgttcact ttaagataaa tatccaacgg tgcttttagc cgcgcatttt
    14101 gcagtgcttt gagctgccag ttgctgtgta cgcaggtggt caggcggtgc tggtcataaa
    14161 tctccagatc ctgagcatgg aaaaatcctt ccagcatcag gatcggtcct ttccagccgc
    14221 gctcacgtaa cgttattgcc tcttccaggt taagtaatgc aaagccatcg gtagccccga
    14281 gcgcgctcca gatacgctca ataccgtgcc cgtaagcgtt cgcttttacc accgaccaga
    14341 cgcgcgcgtg cggcgcggcc tggcggacaa tggacagatt ctgttttaat gcctgcagat
    14401 cgaggctggc ctgtatcgga cgggtcatct cgtttcctta gctgtgtgcg ccatgtaaat
    14461 ggcccggacg tgatggcgta aatccacggc tgtagcgcgc tacgcttaga tcctcatatg
    14521 ggatcgctgg tgtgcgacca gagagcaggt cgcttaacaa ctgaccggaa ccgcaagcca
    14581 tcgtccagcc gagcgtgccg tgaccggtat tcagccacag atttttaaag cgtgtacgcc
    14641 cgacaaccgg cgtgccgtct ggcgtcatcg ggcgcagacc cgtccagaaa gtcgcctgct
    14701 cgacatgacc gccgcgtgga tagagatcgc gaaccaccat ttctagcgtt tcacgacgcg
    14761 gttgcaacag ctcggtgtta aaaccaacaa tttccgccat cccgccaaca cgaatgcggt
    14821 tatcgaaacg ggtaatggcg attttgtagg tttcatcaag aatggtggat actggcgcac
    14881 catcttcctg cgcaattgga atagtcagcg agtagccttt cagcggatac accggaatat
    14941 caacaatgcc tttgagcatc gccgtcgagt aagaaccaaa cgccatcaca tacgcatcgg
    15001 ccttaatcac ttcatcgcca cacttcacgc cgtagatttg ctcgccgtcg caaagcagtt
    15061 ggtcaacggg cgtattaaag cggaatttaa cccccgcctg ctccgccatc cgcgccagat
    15121 tctgggtaaa tagctgacag tctccggttt catcattggg taactgcagg ccgcccgtca
    15181 gtttgtgcgc cacttctgcc agcgcgggct ccacttccgc caggcggctg gattccagca
    15241 gctgatacgg tacgccggca tcttccagca cggcgatatc gcgggtcgca ttttcatact
    15301 gttgttcggt acggaacagt tgcagcgtcc caccctgacg cccttcatac tgaatattgg
    15361 tttcggcgcg caatgctttc aggcaatcac ggctgtattc cgccagacgc accatccgcc
    15421 ctttgttttc catgtagtgg ctggtgtcgc agttacgtaa catttgccac atccatttca
    15481 actggaactg cgtaccgtcg agacgaactg ccagcggcgc atggcgctgg aacatccatt
    15541 tgatcgcttt taaaggcaca cctggtgccg cccacggtgc agcatatccg ggggagattt
    15601 gcccggcatt cgcggcactg gtctccaggg cggcccccgg ctcccgatca atgacggtga
    15661 cctcatgtcc tgcctgattt aagtaccagg cgctggcaac gcctaccaca ccacttccca
    15721 gtatgacaac tcgcatagcc actccgataa cggtagatac agtaaaagaa taataatcta
    15781 attacatctt gataactcag ttgaaaatat tattcaacat acggcttttt tatggtgagc
    15841 tggctcacat ctccctgact ctgcggcaat atggcgtctt tagtatctcc tgctgaacgc
    15901 cctttttatc cagcataaaa ttccgttcag aagcggatta gtggcactct gataataaga
    15961 aatcgcaaag aaaaaaattc tccggcgacg cggtttttaa caggtgttct atgcttgaaa
    16021 tgaggtgctc cacacagaga gcgccgacaa caatgaggtg cgcgtatggc gacgatcgat
    16081 tctatgaata aggacaccac acgtttgagc gatggacccg actggacgtt cgacctgctg
    16141 gatgtttatc tggcagagat agaccgggtg gcgaaactct accggctgga tacctacccg
    16201 caccagattg aagtgataac ctcagaacag atgatggatg cctactccag cgtcggcatg
    16261 ccaattaact atccgcactg gtcattcggt aaaaagttta tcgagactga acggctgtat
    16321 aagcacggtc agcaaggact ggcctatgaa atcgtcatta actctaaccc gtgtatcgct
    16381 tacctgatgg aagagaacac cattaccatg caagcgctgg tgatggctca tgcctgctat
    16441 gggcataact ctttcttcaa aaacaattac ttattccgta gctggaccga cgccagttcg
    16501 attgtcgatt atctgatttt tgcccgtaaa tatattaccg agtgcgaaga gcgctatggc
    16561 gttgatgaag tagaacggct tctggactcg tgccacgcgc tgatgaacta cggcgtggac
    16621 cggtacaaac gcccgcaaaa aatctcgctg caagaagaga aagcccggca gaaaagtcgc
    16681 gaagagtatc tgcaaagtca ggtcaatatg ctctggcgta ccctgccgaa gcgcgaggaa
    16741 gagaaaacgg ttgctgaagc gcgccgctat ccgtccgagc cacaagaaaa cctgctctat
    16801 tttatggaga aaaatgcgcc gctgctggaa tcatggcagc gtgaaatcct gcgtattgtg
    16861 cgtaaggtga gccagtattt ttatccgcaa aaacagactc aggtgatgaa cgaaggctgg
    16921 gccaccttct ggcactacac catccttaac catctgtatg atgaagggaa agtaacggaa
    16981 cgttttatgc tggagttttt gcacagccac accaatgtgg tcttccagcc gccctataac
    17041 agcccgtggt acagcggcat caacccgtat gccctcgggt tcgccatgtt ccaggatatt
    17101 aaacggattt gtcagtcgcc aacggaagaa gacaaatact ggttcccgga tatcgccggt
    17161 tccgactggc tggaaacgct gcatttcgcg atgcgtgatt tcaaagatga gagttttatc
    17221 agccagttcc tgtcaccgaa agtgatgcgt gatttccgct tcttcaccgt gctggatgat
    17281 gatcggcata attatctgga gatttccgct attcataatg aagagggtta tcgggagatc
    17341 cgtaaccggt tatcgtcgca atataactta agtaatctgg agccgaatat tcagatctgg
    17401 aacgtggatt tgcgcggcga ccgttcgctg acactgcgtt acattccaca taatcgcgca
    17461 ccgctggatc gggggcgcaa agaagtactg aagcatgtgc atcacctgtg gggatttgat
    17521 gtgatgctgg aacagcaaaa cgaagacggc agcgtcgagt tgctggaacg ttgcccgccg
    17581 agaatgggaa atctgtaaaa acaacaaaaa acccctcgtt tgaggggttt gttttttaaa
    17641 cggaagggat tatcgcccct gaatggctaa atcacccggc agatttttct gcatccggtg
    17701 ccaaatctcg ccactctcat gcccatagcg acgcactgtt tcgtacacct gatcgtgcgc
    17761 gccttcactg cacaacgccg acagtttgtg gtagaagccc agcgccagac tgcgcgcttc
    17821 cggattggcg aaatagtgac gaccaatgcg cgtatacagt cccttcatcc cgttaaggat
    17881 cagaccgtaa atcgggttgc cggaagcaaa cgccaggccg cggaatatgt tgtaatccag
    17941 ctcggcaaag gcatcggcgt gatcggccac ttcattagcg gtagccagca cttcctgcgc
    18001 tttatcggga tgctgacgaa acgcggtgcg aataaaaata gttgaaatat tggtacgcac
    18061 cgacagcaaa ttatcaataa gctgcggcac actttcgtga tccagtcgcg ccagtgtttc
    18121 aaggatattt aaaccagagg tttcccagaa attattcacc ttcgtcggct tgccatgttg
    18181 aatggtcaac cagccatctc gtgccagacg ctgtaacact tcacgtaacg tagtacgcgt
    18241 tacgccaatt aattctgaaa gttcacgttc tgcgggcaaa atagtcccgg gagggaagcg
    18301 gttattccag atactttcaa taatgtactc ttccgcgaaa cccgccgggc tttgcgcctt
    18361 aatgaccata gtgagatttc cataacacag caaaacaaag ttggactcat cataccagag
    18421 ggctaactac gctgatagca gacaaaaaga agaaaaaagg gatcaaggct tcattttccg
    18481 catctctttc cccttgacta tcaagacttg cctcactcct gcctgaccgt taagctttgc
    18541 gtttaaacat aaaaacatca ttgattattt gtaaggtaag ggaaaccatc atggagatct
    18601 cctggggccg cgcgctatgg cgcaattttt tgggccagtc ccccgactgg tacaaactcg
    18661 ccctcatcat tttcttaatc gtaaatccgt taattttcct catcagccct ttcgtcgctg
    18721 gctggttgct ggtagcggaa tttattttca ctctggcgat ggccctgaaa tgctacccgc
    18781 tactccccgg tggtctgttg gctatcgaag cggtattcat cggcatgacc agcgcggaac
    18841 acgtccgtga agaggtggcg gcaaatcttg aagtcttgct gttattgatg tttatggtgg
    18901 cgggtatcta ttttatgaaa cagctgttgc tgttcatatt tacccgtttg ctgttaagca
    18961 ttcgctccaa aatgctgctg tcgctctctt tttgcgtggc ggcagcattc ctctccgcgt
    19021 tcctcgatgc cttaaccgtc gtggcggtgg tgatcagcgt tgcagtcggt ttttatggta
    19081 tttatcatcg cgtagcctct tcccgtaccg aagacaccga cctgcaagac gatagtcata
    19141 tcgacaagca ttacaaagta gttttggaac agttccgtgg ctttctgcgt agcctgatga
    19201 tgcatgccgg tgtcggcacc gcattaggcg gcgtgatgac catggtgggc gaaccacaga
    19261 acctgatcat cgctaaagcg gctggctggc attttggcga tttcttcctg cgcatgtcgc
    19321 cggtgaccgt tccggttctg atttgtggcc tgttaacctg cctgctggta gagaagctgc
    19381 gttggtttgg ctacggtgaa acgctgccgg agaaagtccg cgaagtgctg caacagtttg
    19441 acgatcaaag ccgccaccag cgtacccgtc aggataaaat ccgtctgatt gtccaggcga
    19501 ttattggcgt ctggctggtg actgcgctgg cgttgcatct ggcggaagtt ggcttgattg
    19561 gtttgtcagt cattattctg gcaacatcat tgaccggtgt caccgatgaa catgctatcg
    19621 gtaaagcctt caccgaatct ctgccattca ccgcactgtt gacggtgttt ttctcggtag
    19681 tcgcggtgat tatcgaccaa caactgtttt cgccgattat tcagtttgtg ttgcaggcat
    19741 cggaacatgc tcagctgtcg ttgttctata ttttcaacgg actgctgtca tccatttcgg
    19801 ataacgtctt cgtggggacg atttatatca acgaagcgaa agcggcaatg gaaagtggcg
    19861 ctatcacgtt gaagcaatac gagctgctgg cggtcgccat taataccggt accaatctgc
    19921 cctctgtcgc tacgccgaac ggtcaggctg cgttcctgtt cctgctgacc tctgcactcg
    19981 cgccattgat tcgcctctct tatggccgca tggtgtggat ggccctgcct tacaccctcg
    20041 tcctgacact cgtcggactg ctctgcgtcg agtttacgct tgcccctgta accgaatggt
    20101 ttatgcaaat gggctggata gcaacgcttt gataacaact taccgggcat ataaatgccc
    20161 ggtttgcctt ttcgcccgat aattgtccaa ttgcgtccta tttttgctgc ctcctggtgg
    20221 cgcagcgaat gaattggttt aaactgcgca ctctatgcat attgcaggga aatgattatg
    20281 ttgcgatttt tgaaccaatg ttcacaaggc cggggcgcgt ggctgttgat ggcgtttact
    20341 gctctggcac tggaactgac ggcgctgtgg ttccagcatg tgatgttact gaaaccttgc
    20401 gtgctctgta tttatgaacg ctgcgcatta ttcggcgttc tgggtgctgc gctgattggc
    20461 gcgatcgccc caaaaacgcc actgcgttat gtagcgatgg ttatctggtt gtatagtgcg
    20521 ttccgcggtg tgcagttaac ttacgagcac accatgcttc agctctatcc ttcgccgttt
    20581 gccacctgtg attttatggt tcgtttcccg gaatggctgc cgctggataa atgggtgccg
    20641 caagtgtttg tcgcctctgg cgattgcgcc gagcgtcagt gggatttttt aggtctggaa
    20701 atgccgcagt ggctgctcgg tatttttatc gcttacctga ttgtcgcagt gctggtggtg
    20761 atttcccagc cgtttaaagc gaaaaaacgt gatctgttcg gtcgctaatc cattcggcgc
    20821 tcctgcggga gcgctttttt cctgccgcta tatttatttg acccgcagta aatcagaact
    20881 tcgcgttgta taacgtggtg aaagcatggc tcgcttcatc tgccattgtt gctggatccc
    20941 ctgcccggca aaatagagtg ttcctctgcc ctctttagca ttcagtgtat ccattaccgt
    21001 catcaattgc tcactcccgg ggcgcggtgc gttgtcatcg aataaattga gctgcgcgac
    21061 tccctgactg aagaaatccc ccagcatcac gcccgctttt tggtagcgat ggcccgcttg
    21121 ccagatggca tccagagatc gcgtagcggc gttaatgata tccctgctgt cctgagtggg
    21181 cgtcagcagt tttaccgacg cgctattgcc gtaataaggt tcattgagcg caaatggtga
    21241 cgtcttaata aatgtggaga taaaccgaca atattgatgc tcactgcgaa gtttttccgc
    21301 cgcccgggca gcgtaactac aaatggcctg ccgcatcgac gtataatccg tgatgcgttc
    21361 accaaacgag cgggaacaga taatttcctg ctttgtcggt gcaaattctt ccagttgcaa
    21421 acagggttca ccgcgcagtt cacgcaccgt tctttcgagc acgacattaa aatgtttacg
    21481 gataaaccgg atatctgtat ccgccaaatc gagaacggtt ttgatcccca tcgcgtccag
    21541 ttttttgctg atccgccgtc caatccccca gacgtcatcc acggggagag cagacattaa
    21601 tttacgctgg cgttccagat ttgataaatc caccacccca cccgtctgcc gctgccattt
    21661 ttttgccgca tgattggcaa gcttagccag cgttttggtc tgggcgatcc ccacaccaac
    21721 agtaagatgg gtacgttgta gcaccgttgc gcgaatttct ctgccaaagt cagtcagatc
    21781 gcgacaatta cgcacacctg tcagatcgca gaatgcctca tcaatactgt aaatctcgac
    21841 gcggggcgat agttcttcca gcgtcgacat cacccgattg ctcatgtctg cgtaaagctc
    21901 ataattgctg ctaaagcaaa ccacgccaca gcgacgaaac agatcttttt gtttgaacca
    21961 gggatcgccc attttaacgc caagcgcctt tgcctcagcg tttcgggcga taacgcaacc
    22021 gtcattattc gatagcacaa ccaccggttt accccataaa tcagggcgaa acaccgtctc
    22081 acagctggca taaaacgcgt ttacatcaca gagggcaaac atcagcgcat cgccttaacg
    22141 acgtggatca ccacaccaaa gacatccagc gtatcttcac tactgatggt aatgggcgag
    22201 tacgcgctgt tcatgggaat aagctgtacc gtcgggcgta gttgcaattt tttcaccgta
    22261 aactcgccgt caacagcagc gatgacaata tcaccatggc tggcggtaat agcgctatcg
    22321 acaatcagta aatcaccgtc actaattcca ccatcaatca tagaatcacc acttgctttg
    22381 acgaagtaag tcgcgctggg atgctggatc aacagttgat tcagatcgat gcgctgttca
    22441 acgtaatctg cggccggtga aggaaagcca cactgaacaa gatcgctaaa tagcggaaaa
    22501 gtcacaattt cgcggagatc cgcaggcttg ataaacaaca taataatctg cctgaagttg
    22561 tactgttttt atatacagta gtctgttctt gccagcagat caatactgat tcaggctatc
    22621 aatatttgtc gctgcataga ctgctgattt ttcgttctct tatcctgtgc tcacgcggcc
    22681 ttctggcgac gacgctcatc cagcagaaat gaaaaatatc acccggctaa aaaatagaat
    22741 agaagcattc gccataatga cattaaacat tgtttgatat ttatcatatt aatataaata
    22801 aagaca
//
LOCUS       DAZGBC010000076        22746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGBC010000076 DAZGBC010000000
VERSION     DAZGBC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.076"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(530..1621)
                     /gene="ychF"
                     /locus_tag="ACHK1J_002781"
     CDS             complement(530..1621)
                     /gene="ychF"
                     /locus_tag="ACHK1J_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO7912025.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1738..2322)
                     /gene="pth"
                     /locus_tag="ACHK1J_002782"
     CDS             complement(1738..2322)
                     /gene="pth"
                     /locus_tag="ACHK1J_002782"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO7912026.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2600..2878
                     /gene="ychH"
                     /locus_tag="ACHK1J_002783"
     CDS             2600..2878
                     /gene="ychH"
                     /locus_tag="ACHK1J_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO7912027.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2933..4612)
                     /gene="dauA"
                     /locus_tag="ACHK1J_002784"
     CDS             complement(2933..4612)
                     /gene="dauA"
                     /locus_tag="ACHK1J_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO7912028.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4737..5684)
                     /gene="prs"
                     /locus_tag="ACHK1J_002785"
     CDS             complement(4737..5684)
                     /gene="prs"
                     /locus_tag="ACHK1J_002785"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO7912029.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5835..6686)
                     /gene="ispE"
                     /locus_tag="ACHK1J_002786"
     CDS             complement(5835..6686)
                     /gene="ispE"
                     /locus_tag="ACHK1J_002786"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO7912030.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVAPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6686..7309)
                     /gene="lolB"
                     /locus_tag="ACHK1J_002787"
     CDS             complement(6686..7309)
                     /gene="lolB"
                     /locus_tag="ACHK1J_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO7912031.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7523..8779
                     /gene="hemA"
                     /locus_tag="ACHK1J_002788"
     CDS             7523..8779
                     /gene="hemA"
                     /locus_tag="ACHK1J_002788"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO7912032.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8821..9903
                     /gene="prfA"
                     /locus_tag="ACHK1J_002789"
     CDS             8821..9903
                     /gene="prfA"
                     /locus_tag="ACHK1J_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO7912033.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9903..10736
                     /gene="prmC"
                     /locus_tag="ACHK1J_002790"
     CDS             9903..10736
                     /gene="prmC"
                     /locus_tag="ACHK1J_002790"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7912034.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10733..11125
                     /gene="sirB2"
                     /locus_tag="ACHK1J_002791"
     CDS             10733..11125
                     /gene="sirB2"
                     /locus_tag="ACHK1J_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO7912035.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11129..11938
                     /gene="sirB1"
                     /locus_tag="ACHK1J_002792"
     CDS             11129..11938
                     /gene="sirB1"
                     /locus_tag="ACHK1J_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO7912036.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11974..12828
                     /gene="kdsA"
                     /locus_tag="ACHK1J_002793"
     CDS             11974..12828
                     /gene="kdsA"
                     /locus_tag="ACHK1J_002793"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO7912037.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12977..13084)
                     /locus_tag="ACHK1J_002794"
     CDS             complement(12977..13084)
                     /locus_tag="ACHK1J_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7912038.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13513..13620)
                     /locus_tag="ACHK1J_002795"
     CDS             complement(13513..13620)
                     /locus_tag="ACHK1J_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7912039.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(14025..15125)
                     /gene="chaA"
                     /locus_tag="ACHK1J_002796"
     CDS             complement(14025..15125)
                     /gene="chaA"
                     /locus_tag="ACHK1J_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO7912040.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15395..15625
                     /gene="chaB"
                     /locus_tag="ACHK1J_002797"
     CDS             15395..15625
                     /gene="chaB"
                     /locus_tag="ACHK1J_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO7912041.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15783..16478
                     /gene="chaC"
                     /locus_tag="ACHK1J_002798"
     CDS             15783..16478
                     /gene="chaC"
                     /locus_tag="ACHK1J_002798"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO7912042.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTALQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16522..16875)
                     /gene="ychN"
                     /locus_tag="ACHK1J_002799"
     CDS             complement(16522..16875)
                     /gene="ychN"
                     /locus_tag="ACHK1J_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO7912043.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            17061..18455
                     /gene="ychO"
                     /locus_tag="ACHK1J_002800"
                     /gene_synonym="iatA"
     CDS             17061..18455
                     /gene="ychO"
                     /locus_tag="ACHK1J_002800"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO7912044.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18456..19106)
                     /gene="narL"
                     /locus_tag="ACHK1J_002801"
     CDS             complement(18456..19106)
                     /gene="narL"
                     /locus_tag="ACHK1J_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO7912045.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(19099..20895)
                     /gene="narX"
                     /locus_tag="ACHK1J_002802"
     CDS             complement(19099..20895)
                     /gene="narX"
                     /locus_tag="ACHK1J_002802"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO7912046.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21234..22625
                     /gene="narK"
                     /locus_tag="ACHK1J_002803"
     CDS             21234..22625
                     /gene="narK"
                     /locus_tag="ACHK1J_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO7912047.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5596 a         5675 c         5860 g         5615 t
ORIGIN      
        1 taatcctaat tagttatggg attttcgctc gatgtatctg cataacaaaa acaatatgcg
       61 ccatttttgc attactaata agaaaaagca aaaaacatcg atattcattt ttgatcaatg
      121 atttttctgg catatcgtta ttatcgtgct attggttatc attttcatct tgccaggatc
      181 gcacgtaatc tcattattcg gggactttat ctgcgggata ttacccttca ggtaagtcct
      241 tttctcgcca gcatattgga gcgtgatcaa tattgatcag ctgtgaaggg ggtgatgtgt
      301 agtggaacaa aaacttacgt tatatcacta ctaatgagat atccgtcatc tgaacactga
      361 tgtctttaat tattctggtg gttcactgta ggcaataagc acaaaagtgt aggatgttac
      421 aagaatgatt aggactcggt gaaatgaaaa atccacgcaa ttgcgtggat tttatatact
      481 tttgcgctct tcatgagatt tagcgaaacc tcatgagaca acaaattaat tagacgttga
      541 aaaggaagtt catcacatcg ccatctttaa cgatgtaatc tttaccttct gcacgcattt
      601 tgcctgcttc tttcgcgcct tgttcacctt tgtaagtgat gaaatcttca aacgagatgg
      661 tttgtgcacg gataaagcct ttttcaaaat cagtatggat tttgcccgct gcctgcggcg
      721 cggttgctcc aactggaatg gtccatgcac gcacttcttt caccccagcg gtgaagtaag
      781 tttgcaggtt cagcagctta taaccggcac ggatcacacg gttcaggccc ggctcttcca
      841 gcccaagctc ctgcataaac tcgtcacgtt cttcgtcgtc cagttcggca atgtctgctt
      901 caacagcggc acaaaccgga accacaacag aaccttcttt cgccgcgatt tcacgcacct
      961 ggtcaagata tgggttgttt tcaaaaccgt cttcgttgac gttggcgatg tacattgttg
     1021 gttttagcgt caggaagctc aggtaacgaa tagccgcttt ctcttcagcg cttaaatcca
     1081 gcgcgcgcag catacctgcg ttttccagct ggggcaggca tttttccagg accgccagct
     1141 cagctttcgc gtctttatcg ccacctttgg ctttcttctg tacgcgatga atcgcacgtt
     1201 cgcaggtgtc gaggtctgcc agcgccagtt cggtgttgat aacttcaata tcgtcagccg
     1261 ggttaacttt gccggaaacg tgaatgatgt tgtcattttc aaagcagcga acaacgtgac
     1321 cgatcgcttc ggtttcacgg atgttggtca ggaactggtt acccagacct tcgcctttcg
     1381 atgcgccttt taccagaccg gcgatatcga caaattccat ggtcgtggga agcgtacgtt
     1441 gcggttttac gatttcagcc agttgatcca ggcgaggatc aggcattggt acgacgcctg
     1501 tgttcggctc aatggtgcag aatggaaagt tggccgcttc aataccggct ttggtcagcg
     1561 cgttgaacag ggtagatttc ccgacgttgg gcaaaccgac gataccgcat ttgaatccca
     1621 tgatttaaat caccttaata tcttaataat caacctgtta ttgctaacag attgcagaaa
     1681 tggaaataac tttgcctatt atacacggca ctcgacaaaa atgccgcaga caacgactta
     1741 ttgcgcttta aaggcgtgca atcggttcgt tgctttggtc aagccatctg taaaccacat
     1801 ttcagtacaa cgcgccgctt cgtcgatggc ttcatcaatt aacttctgtt cactaacagg
     1861 cggtttgcct aacacaaaac cgacaacttt atttttatcg cccggatgac cgattccgat
     1921 gcgtaaacgg tgaaagttag ggttattacc caatttactg atgatgtctt tcagtccatt
     1981 gtgaccacca tggccaccgc ccaacttaaa tttggcgacg ccaggaggca gatccagttc
     2041 gtcgtgggcc accagaattt cgtccggatt aatgcggaaa aaactggcca tcgccgcaac
     2101 ggctttgccg ctgagattca taaatgtagt cgggactaac aggcggacat cttcgcctcc
     2161 aagggtgact cgcgaagtat aaccaaagaa tttagcctct tcgcgcagcg gagcgcgcaa
     2221 acgctctgcc agtaagtcaa cgaaccaggc accagcatta tgtcgcgttg cggcgtattc
     2281 agcaccgggg ttcgccaggc cgacaatcaa tttaatcgtc acgttttttt gtcctgagtg
     2341 tgtacataac tggcgcgtag tttactggtt gcggccccgc ttgacaaaaa actgcgtatc
     2401 aaatgcagat aacgtaataa ttgcctgagt ggactattag aaagtcaggg tgttcaggcg
     2461 tttatttgta aagttttgtt gaaataaggg ttgtaattgt gatcacgccc gcacataacc
     2521 cacagggtgt tgtctatact ttacacataa ggaagagggg tattccctgt tacaacccag
     2581 aaagttccgg aggtgacata tgaaacgcaa aaacgcttcg ttactcggta acgtgctcat
     2641 ggggttgggt ctggtggtaa tggtggtcgg cgtggggtat tcaatcctca accagttacc
     2701 acagtttaat atgccccagt atttcgcaca tggtgcagtg ctaagtattt tcgtcggtgc
     2761 cattctctgg ctggcgggtg cccgtgttgg cgggcatgaa caggtgtgcg accgttactg
     2821 gtgggttcgc cactatgaca aacgttgccg ccgtagcgat aatcgccgtc atagctaaca
     2881 aatactgcag tttgctgacc agtcagtttg cgctgactgg tcaatctcga acttataaat
     2941 ccgccatcgc cgctcgacga ttcgggaaga acgcgaggcg tcccgggatc ggttgaatgc
     3001 ctgcgcgagc catagtgcgc agtggctgga attccacgtt gcacacgcgc agttcacatc
     3061 cttcgggcag acgcttcaca aaacgctgga acgcatcaag accaccagca tcaagtaccg
     3121 gaacggcatc ccacttcaga atcacaatcc gtttgccttc aagacgtgac tccaggtccg
     3181 tgaataagcc ttcagcagca gcaaaaaaca gcgggccaat aacgcgcaga accaggacat
     3241 cgtctggaac atctacgact accggtgcca ggcgagtcat acgtgcgata cgacgcataa
     3301 acagcagcga tgccagcacg atccccacgc tgatggcaat aaccatatca aacagcacgg
     3361 tcagcgacat gcacagcagc atgacaatga tgtcatcttt cggtgcatga cgcagcaagt
     3421 cgaccacttt atgcgcttca ctcatgttcc acgccaccat caacagcagg gctgccatag
     3481 cggaaagcgg cagccaggag agcagcggtg ccagtaccag cagggcaaga ataaccagaa
     3541 tagagtggat caccgccgag ataggggaag ttgccccggc acggacgtta gcggcagaac
     3601 gcgcgatggc agctgtagcg gtaataccac caaagaacgg agcgatgata ttccccagtc
     3661 cctgtccaac cagttcgcta ttcgccttgt gtttcgtccc ggtcatacca tccagcacca
     3721 cggcgcagag cagagattcg attgcgccga gcattgccat tgagaatgcc gcaggcagca
     3781 gtgtgcgaat agaatcccag gttaacgtga attctgaatt aggcagatcc cacggcagca
     3841 ccagttgcgg cagcagttgc ggaataccgt taccctgaga accatcggcc aggacgtagt
     3901 ggaattgcga accgatggta gcaacatgtc cgccgagcag gttaacaatc cccatcaccg
     3961 cgcaaccagc cagcaaggcc ggaaggtgac cgggtaaacg aatgcccaga cgcggccaga
     4021 aaacaagaat acctagcgtc acaatgccaa tggcagcatc acccacatta atggtcggca
     4081 gcgccataaa taatgcgccg actttttgta gataatgttc cgggacatgg gccatttgca
     4141 gaccgagaaa atctttaatc tgcatggtac cgatggtgat cccgataccc gaggtgaaac
     4201 ctaaggtgac ggaaaccgga atatactcaa tcaggcgacc aaagcgtgcc agacccatca
     4261 gaatcaaaaa gatccccgac agcaaggtcg caaccagcag tcctgccagc ccaaactgtt
     4321 gcgaaacggg atagagaatt accacaaatg ccgcagtcgg accggaaacg ctaaagcgtg
     4381 acccacccgt cagagcaatg acaatccccg caacagctgc ggtatataaa ccgtactggg
     4441 gtgccacacc actaccaata gccaacgcca tcgccagcgg gatagcaata atcccgacgg
     4501 ttatcccggc aatcaggtca cgggtaaacc gtgcggcagt atatttttct ttccagcaag
     4561 cgtcgatcag agcgcggaaa ggcatcacat gtgaggaaaa tattttgttc acaataatgt
     4621 ttcatccgtg agcgcatcat ctgtcaacta aatggcaggt gaaggaggca taggtcatac
     4681 aaatggatat tacagacaaa aaaacccgcc gcagcgggtc tttgagccgg gttcgattag
     4741 tgttcgaaca tggcagagat cgattcttcg ttgctgatac gacgaatcgc ttcggccagc
     4801 atacctgaca gggtcagagt acgcacgttc ggcagtgatt tgatttcatc gctcagcgga
     4861 atggtatcgc agacaacgac ttcatcaatt acagagttac gcaggttgtt cgccgcgttg
     4921 ccagagaaga tcgggtgagt cgcatacgca aatacacgtt tagcaccacg ttctttcaga
     4981 gcttcagcag ctttacacag cgtaccgcca gtgtcgatca tatcatcgac cagtacgcag
     5041 tcacgacctg caacgtcacc gatgatatgc atcacctggg aaacgttcgc acgcggacga
     5101 cgtttgtcga tgattgccat atcggtatcg ttcagcagct tagcgatagc gcgggcacgc
     5161 acaacgccgc cgatgtccgg agaaaccaca attgggttat ccagattcag ctgcagcatg
     5221 tcttccagca ggatcgggct accaaatacg ttatcgaccg gaacgtcgaa gaaaccctga
     5281 atctgttcag cgtgcagatc cactgtcagc acacggtcaa caccgacgct ggagagaaag
     5341 tctgcaacca ctttcgcagt gattggtaca cgagcggaac ggacgcgacg gtcctggcgc
     5401 gcatagccaa agtaggggat aacagcggtg atacgacctg cggaagcacg acgcagggca
     5461 tcaaccataa cgactaattc catcaggttg tcgttagtag gggcacaagt ggactggatg
     5521 atgaaaatat caccaccgcg tacattttca ttaatttgta cgctgacttc gccatcgcta
     5581 aagcgaccta cagcggcgtc gccgagtgaa gtgtacaggc ggttggcaat acgttgtgct
     5641 agttccgggg tggcgttacc agcaaaaagc ttcatatcag gcacgagaag aacctcaggc
     5701 atgcgtccat tggtggaaag aatctgccga aaactgtgcg ggccaggcat gatcctttcc
     5761 aggcggtgta ttaaagagcg cgatgcaacg tctggaacaa ggtgacgttg tcaccgaaac
     5821 tcagcttgcc cggcttaaag catggctctg tgcaatgggg aaagattagc gcctttcgcc
     5881 acaaagccat tgagccattc cggggcttgc tctagcacct ggcgggcttc agactctgta
     5941 tcaaattcag caaagacaca ggcccctgtc ccagtcaggc gcgacggggc gtattctaac
     6001 agccaggaaa gcaccgcatc aacctcgcga aaacgttttc ttgcgataac ctcgcaatca
     6061 ttgctgaatt cacattttag caacgtttct attgaccttt ttggcgtatt gcgcgggagt
     6121 tcaggatctt taaaaatcac cggagtcgga atacttacac cagggtgcgc caccagatac
     6181 cacttctctg gcggagccac cggcgttagt atttcaccaa cgccttcggc aaacgcggca
     6241 tgcccccgaa caaagacagg aacatctgcg cccagcgtca gccccatttc cgccagctca
     6301 tccatgctta gcccgcattg ccagagatga tttaatgcca ccaggaccgt cgcggcattg
     6361 gatgaaccac cgccgagacc gccgcccatc ggcaaacgct tgtcaatgct gatattcgca
     6421 ccgcttcccg tcggaagacg cccgctgtct gccgcagttt tcatcaataa ccgcgctgcg
     6481 cgaacgatca ggttatcttc atgttccacg ccttcaacgg gcgttaacag acgaatatcc
     6541 ccatcgtcac gaagctcaat gctgatggtg tcgccgtaat caagaaactg aaacagcgtt
     6601 tgcagcgtgt ggtaaccatc cgcacgctga ccggtaatgt ataaaaacag attaagtttt
     6661 gccggagagg gccactgtgt ccgcattatt tcactatcca gttatccatt tttaacttga
     6721 tgcgttgacc accgtcggtg agttccatat tggctggcat cgcaggttgc gttttggtgt
     6781 cataaccacc ataaacaacc ttccagtttt tgccattctg gctgtaggta atttcgctca
     6841 ggcggtactg gtcgtccagt ttgtagtcgg ttgcatcacc cggtaaacct aaaatccact
     6901 ggcgcaagct gttgagcgga attggcattc cagtcaattt gccaatcatc tcttcggcgt
     6961 catcggcggt ataacgctga cctttattgt cgactaactg cacgttaccc ggttgagcat
     7021 tcagctccag ttccgtgctg cccaatgggt tagtgagcag cagacggtag cgatcctggc
     7081 cggtttgctg ccagaaaaag cgggcgtaca ctttttgttg gtcagaaata taagcgaacg
     7141 cgccgcgagt ctgatactga ttaagattgc gcacgtcttg ctgatgctga cgccattgtg
     7201 gcgaatccgg gcttttgcca ggacctttgg gcgtggtaac ggaacaggca gtgagcacaa
     7261 gagcagccag cggtagcagg cggataagac gaaaatcggg caggggcata gtgatgacaa
     7321 gtccttgaga tacgttgcag ttataaccct taatgctagc gttaccgtcc gctatcgtct
     7381 atgttcaagt tgtcttaatt gccagaatct aacggctttc ggcaattact ccaaaagggg
     7441 gcgctctctt ttattgatct tacgcatcct gtatgatgca agcagactaa ccctatcaac
     7501 gttggtatta tttcccgcag acatgaccct tttagcactc ggtatcaacc ataaaacggc
     7561 acctgtatcg ctgcgagaac gtgtatcgtt ttcgccggat aagctcgatc aggcgcttga
     7621 cagcctgctt gcgcagccga tggtgcaggg cggcgtggtg ctgtcgacgt gcaaccgcac
     7681 ggaactttat cttagcgttg aagagcagga taacctgcaa gaggcgttaa tccgctggct
     7741 ttgcgattat cacaatctta atgaagaaga tctgcgtaaa agcctctact ggcatcagga
     7801 taacgacgcg gttagccatt taatgcgtgt tgccagcggc ctggattcac tggttcttgg
     7861 ggagccgcag atcctcggtc aggttaaaaa agcgtttgcc gattcgcaaa aaggccatat
     7921 gaaggccagc gaactggaac gcatgttcca gaaatctttc tctgtagcga aacgcgttcg
     7981 cactgaaaca gatatcggtg ccagcgctgt gtctgtcgct tttgcggctt gtacgctggc
     8041 gcggcagatc tttgaatcgc tctctacggt cacagtgttg ctggtaggcg cgggcgaaac
     8101 catcgagctg gtagcgcgtc atctgcgcga acataaagta cagaagatga ttatcgccaa
     8161 ccgcactcgc gaacgtgccc aaattctggc agatgaagtc ggcgcggaag tgattgccct
     8221 gagtgatatc gacgaacgtc tgcgcgaagc cgatatcatc atcagttcca ccgccagccc
     8281 gttaccgatt atcgggaaag gcatggtgga gcgcgcatta aaaagccgtc gcaaccaacc
     8341 aatgctgttg gtggatattg ccgttccgcg cgatgttgag ccggaagttg gcaaactggc
     8401 gaatgcttat ctttatagcg ttgatgatct gcaaagcatc atttcgcaca acctggcgca
     8461 gcgtaaagcc gcagcggttg aggcggaaac tattgtcgct caggaaacca gcgaatttat
     8521 ggcgtggctg cgagcacaaa gcgccagcga aaccattcgc gagtatcgca gccaggcaga
     8581 gcaagttcgc gatgagttaa ccgccaaagc gttagcggcc cttgagcagg gcggcgacgc
     8641 gcaagccatt atgcaggatc tggcatggaa actgactaac cgcttgatcc atgcgccaac
     8701 gaaatcactt caacaggccg cccgtgacgg ggataacgaa cgcctgaata ttctgcgcga
     8761 cagcctcggg ctggagtagc agtacatcat tttctttttt tacagggtgc atttacgcct
     8821 atgaagcctt ctatcgttgc caaactggaa gccctgcatg aacgccatga agaagttcag
     8881 gcgttgctgg gtgacgcgca aactatcgcc gaccaggaac gttttcgcgc attatcacgc
     8941 gaatatgcgc agttaagtga tgtttcgcgc tgttttaccg actggcaaca ggttcaggaa
     9001 gatatcgaaa ccgcacagat gatgctcgac gatcctgaaa tgcgtgagat ggcgcaggat
     9061 gaactgcgcg aagctaaaga aaaaagcgag caactggaac agcaattaca ggttctgtta
     9121 ctgccaaaag atcctgatga cgaacgtaac gccttcctcg aagtccgagc cggaaccggc
     9181 ggcgacgaag cggcgctgtt cgcaggcgat ctgttccgta tgtacagccg ttacgccgaa
     9241 gcccgccgct ggcgggtaga aatcatgagc gccagcgagg gtgaacatgg tggttataaa
     9301 gagatcatcg ccaaaattag cggtgatggt gtgtatggtc gtctgaaatt tgaatccggc
     9361 ggtcatcgcg tgcagcgtgt tcctgctacg gaatcgcagg gtcgtattca tacttctgct
     9421 tgtaccgttg cggtaatgcc agaactacct gacgcagaac tgccggacat caacccagca
     9481 gatttacgca ttgatacttt ccgctcgtca ggggcgggtg gtcagcacgt taacaccacc
     9541 gattcggcaa ttcgtattac tcacttgccg accgggattg ttgttgaatg tcaggacgaa
     9601 cgttcacaac ataaaaacaa agctaaagca ctttctgttc tcggtgctcg catccacgct
     9661 gctgaaatgg caaaacgcca acaggccgaa gcgtctaccc gtcgtaacct gctggggagt
     9721 ggcgatcgca gcgaccgtaa ccgtacttac aacttcccgc aggggcgcgt taccgatcac
     9781 cgcatcaacc tgacgctcta ccgcctggat gaagtgatgg aaggtaagct ggatatgctg
     9841 attgaaccga ttatccagga acatcaggcc gaccaactgg cggcgttgtc cgagcaggaa
     9901 taatggaata tcaacactgg ttacgtgaag caataagcca acttcaggcg agcgaaagcc
     9961 cgcggcgtga tgctgaaatc ctgctggagc atgttaccgg cagagggcgt acttttattc
    10021 tcgcctttgg tgaaacgcag ctgactgacg aacaatgtca gcaacttgat gcgctactga
    10081 cacgtcgtcg cgatggtgaa cccattgctc atttaaccgg ggtgcgagaa ttctggtcgt
    10141 tgccgttatt tgtttcgcca gcgaccttaa ttccgcgccc ggatacggag tgtctggtgg
    10201 agcaggcact ggcgcggttg cctgaacaac cttgccgtat tctcgatctc gggacgggta
    10261 ccggggcgat tgcgcttgcg ctggctagcg agcgcccgga ctgcgaaatt atcgctgtag
    10321 atcgtatgcc tgatgctgtc tccctggcac aacgtaatgc ccagcatctg gcgatcaaaa
    10381 atatccacat tctgcaaagc gactggttta gcgcgctagc cgggcagcag tttgcgatga
    10441 ttgtcagcaa tccgccgtat attgacgagc aggaccctca tcttcaacaa ggcgatgtcc
    10501 gctttgagcc gctcactgcg ctggttgcgg cagacagtgg aatggcagac atcgtgcata
    10561 tcatcgaaca gtcgcgtaac gcgctggtat ccggcggctt tctgcttctg gaacatggct
    10621 ggcagcaggg cgaagcggtg cgacaggcat ttatcctcgc ggggtatcat gacgtcgaaa
    10681 cctgccgtga ctatggtgat aacgagcgcg taacgctcgg ccgctattat caatgacaag
    10741 tttttctaca ctgcttagtg ttcatcttat tagtatcgcg ctttctgttg ggctattaac
    10801 cttacgtttc tggctacgtt atcagaagca tccacaggca tttgctcgct ggacgcgcat
    10861 tgtgccgccg gttgtcgata cgttgttatt gttaagcggc attgcgttga tggctaaagc
    10921 gcacatcctg ccattttccg ggcaggcaca gtggctgact gaaaagctgt ttggagttat
    10981 catttatatc gttttgggtt ttattgcact cgattatcgt cgtatgcaca gtcagcaggc
    11041 gcgcattatt gccttcccgc tggcgttggt ggtgctgtac atcatcatta aactcgccac
    11101 cacaaaagta ccgttactgg ggtaagtcat gagatcgtta gctgatttcg aatttaataa
    11161 agcgccattg tgcgaaggca tgatcctggc ttgcgaagca atccgccgcg attttccctc
    11221 gcaagatgtt tacgacgaac tggagcgtct cgttagtctg gcgaaggaag aaatcagcca
    11281 gcttctgcct ttagaagagc agctggaaaa actgatcgcg ctgttttacg gcgactgggg
    11341 atttaaagcc tcacgcggcg tttatcgtct ttccgatgca ttatggctgg accaggtgtt
    11401 aaagaatcga cagggcagtg cggtatcatt aggtgcggtt ttattatggg tcgcgaatcg
    11461 tctcgatttg ccgctgctgc cggtgatttt ccctacgcag ctgatattgc gcattgaatg
    11521 tccggatggc gaaatttggc tgattaatcc ttttaacggt gaatcgttaa gcgaacatat
    11581 gctggacgta tggttaaagg gaaatatcag cccgtcggcg gaactgtttt atgaagacct
    11641 tgatgaagct gataacattg aggtaatccg caaattgctg gatacactca aagcctcgtt
    11701 gatggaagaa aatcagatgg agctggcgtt acgcaccagc gaagctttat tacaattcaa
    11761 cccggaagat ccctatgaaa ttcgcgatcg cgggttgatt tatgcgcaac tggattgcga
    11821 acacgttgcg ttgaacgatt taagttattt cgttgaacag tgtccggaag acccgatcag
    11881 cgaaatgatc cgtgcgcaaa taaataacat cgcgcataaa catattgtgc tgcattaatt
    11941 aatcgacatt ttactcaagg ttaaggcgat cctatgaaac aaaaagtggt tagcattggc
    12001 gacatcaacg tagcaaatga cctgccgttc gtactgtttg gcggtatgaa cgtgctggaa
    12061 tctcgcgatc tggcgatgcg catttgcgag cactacgtaa ccgtgaccca gaaactgggt
    12121 atcccttacg tgttcaaagc ctcttttgac aaagccaacc gctcctccat ccactcttat
    12181 cgtggaccgg gcctggaaga agggatgaaa atcttccagg agttgaagca gacttttggc
    12241 gtgaaaatta tcaccgacgt tcacgaacca agtcaggcac agcccgttgc tgatgtcgtg
    12301 gatgtgattc agttgccggc gtttcttgct cgccagactg acctggttga agccatggcg
    12361 aaaaccggtg cggtaattaa cgtcaagaaa ccacagtttg tcagcccggg acagatgggt
    12421 aatatcgttg ataaattcaa agaaggcggc aacgaaaaag tgattctttg cgatcgtggt
    12481 gctaacttcg gctatgacaa cctggttgtc gatatgctgg gcttcagcat tatgaagaaa
    12541 gtgtctggta actcgccggt gattttcgac gtgacccacg cattgcaatg ccgcgatcct
    12601 tttggcgcag cttccggcgg tcgtcgtgct caggtggctg agctggcacg agccggtatg
    12661 gcggtaggtc tggcggggct gtttattgaa gcgcatccgg atccggaaca tgcgaaatgt
    12721 gatggtccat ccgcgctgcc gctggctaaa ctggaaccgt tcctcaagca gatgaaagcg
    12781 attgatgatc tggtgaaagg tttcgaagaa ctggatacca gcaagtaatc ttttttgctt
    12841 gaaaaataaa gtcttagcgt tctgcgttaa gacttttttc atgggtgccg gatacaaaaa
    12901 aggccgcagg ctgttatccc tgcggccggt ttcgggcgca tattgccatc acggcagcct
    12961 gacgcccgtt ttcgccttac ttccggttac gccaccagcc gacaatcgct gcggtaataa
    13021 ttcccgccag gatcggtgct gccaggtcgt gccagaaaat catggcaaac tgcgcgagcg
    13081 tcatatagcc gccttgttgt aatgacaaca ttttgcggct attcttgcat tgttgatggt
    13141 tcaagattag cccccgttct gttgtcaggt tttacctctc aacgtgcggg ggttttctct
    13201 ttccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc accggataac
    13261 gccggcttgg tgtggatact acgactcaat tcatcttcac ttcatccctg aaatgtttgc
    13321 aatgaagagt gcattccggt ttttcaacag ctgttacagt cgttttatga gtgctctgga
    13381 tgatgcttcc agctcgggtt gccaatattt acttgtggaa gtgataaaga caaaaatggc
    13441 cgcaggctgt tacccctgcg gccggtttcg ggcgcatatt gccatcacgg cagcctgacg
    13501 cccgttttca ccttacttcc ggttacgcca ccagccaaca atcgctgcgg taataattcc
    13561 cgccaggatc ggtgccgcca ggtcgtgcca gaaagtcatg gcaaactgcg cgagcgtcat
    13621 ataaccgcct tgttgtaatg acaacatttt gcggctattc ttgaagtgtc tggtttcaag
    13681 attagccccc gttctgttgt caggttttac ctctcaacgt gcgggggttt tctctttcca
    13741 gcaaccaatg ccaccaggga taaagccccc gcaacattgc gcctcaccgg ataacgccgg
    13801 cttggtgtgg atactacgtc tcaattcatc ttcacttcat ccctgaaatg tttgcaatga
    13861 agagtacatt ccggcttttc aacagctgtt gcagtggttt cgtgcaggtt gtggtacagg
    13921 ctcgcaattc tgattaacga cctgtccagg tagtatgaag aaatacaata agcaggaatt
    13981 tatattatct cccccgattt ccgtcatcgg aggagatata actgtcaggc aaatatcgtc
    14041 atcaaatagg cggcaaacag tgccagatgc gctgcgccat tgagcacgtt agtacgtccg
    14101 gtggagaagg agatatggca cagcactaaa gaggccacca tcaccaccat ttctggcgca
    14161 ccaagtgcaa actgcaattc gttacccgtc ataaatgcaa ttagcgtgac gacaggtacg
    14221 gtaagcgaaa ttgttgctaa cactgaacca aagaacagat tcatcgcgcg ctggacctgg
    14281 ttgttcaaca ctgcttttaa tgcacctaaa ccttccggcg acagaatcaa cagtgccacc
    14341 aggaagccag taaaggcgac aggggcattc atgctgtcga gcaaagtctc cagcgagctg
    14401 gcgttcattt tggtcaccgc aataacggca atcagatgga taatcaacca gatagcatgc
    14461 cacaggctgc tatgggcaga cggtttaccg tggtgcgggt cgtcgtcatc actgtcatct
    14521 tcgtgctcgt agacaaacaa actttgatgc gttttggtct ggatcagcaa aaatacgcca
    14581 tacattgccg cagaaattaa tgctaccagt aacgcctgac cggttgaaaa attcgccgca
    14641 ggcagagcca ttggaaatac cagtacgatt atcgccaggg ggaacagcgc aattaaatac
    14701 tgcttgatac caaacagatt catatattgg gtggcaaact tacgaccgcc caacaataat
    14761 gaaaagccaa ccagcccacc ggtaacaatc ataatgattg aatagagcgt atcacgcatt
    14821 agcgttggcg cggcgtcgcc ggttgccatt aaagctgaaa tcaaactgac ttcaagaata
    14881 accactgaaa ggctaagaat aagcgaaccg taaggttctc ccaggcgatg ggctaatacg
    14941 tccgcatgac ggacaacact aaaggcgcta cttaaaatac caataagcgc aagaagattg
    15001 atggcaatga ccactggtag tgtctggctg cttccccaca ggaacagcac taccagcgcc
    15061 agaaccggga aaataagcga agtctccttg tggcgggttt ttaccgcctc ttgagcattt
    15121 gacattatgg ttatcccttt gcagatgaat ttgtcgaaaa tgtaaaaaat aggtaggaaa
    15181 aataacagaa agtgtctgga tatcggtaac attttacgaa attttacccc gctgtcgatt
    15241 ttttactcat tggggcataa aaataagtaa tacgtttaga caatgtttgt tttagcgttt
    15301 cttaagaaca gtctgacatg aaaattctta tgttttggca agagtagata ttgttgacca
    15361 cacttaatgt tcaactttgt aaaaggagtc aacgatgccg tataaaacga aaagcgacct
    15421 gccggaaagc gtaaagcacg ttctaccgtc tcatgcccag gatatctata aagaagcgtt
    15481 caacagcgca tgggatcaat ataaagataa agaagatcgg cgtgatgacg ccagtcgcga
    15541 agaaaccgcg cataaagtgg cctgggctgc tgtgaagcat gaatatgcca aaggggatga
    15601 tgataaatgg cataaaaaat cgtaaaaccg gtagcttagt taaagctatt cgtgcggtgt
    15661 tgccttgcaa gtggtccgcg gattgcatat tgtcccgtta gtggtttcaa aatgagcagt
    15721 aaaaatgtcc ggaagacacc aaaaagttgt cgcagggaag catgcagtgg cggaggtgta
    15781 aggtgataac gcgtgatttc ttgatgaatg ccgattgtaa aacggcattt ggtgccattg
    15841 aagaatcact cttatggtca gcagaacaac gggcggcttc gctggcggcg acgctggctt
    15901 gtcgacctga tgagggacca gtgtggatct tcggttatgg atcgttgatg tggaatccgg
    15961 cactggagtt taccgagtcg tgcaccggta cactggttgg atggcatcgt gcgttttgcc
    16021 tgcgtctgac cgccgggcgc gggactgcgc tccagccggg acggatgctt gcactgaaag
    16081 agggcggacg caccacaggc gtcgcctatc gactgccaga agagacgctg gagcaggaac
    16141 taaccctgtt gtggaagcgc gagatgatta ccggctgtta tctgccaacc tggtgtcagc
    16201 ttgatcttga tgatggacgc acagtaaacg ccattgtgtt tattatggac ccgcgacatc
    16261 cagaatatga atctgacact cgcgctcagg tcatcgcgcc gttgattgcg gcggcgagcg
    16321 gtccgctggg aaccaacgca caatacctgt tttcactgga acaggagctt atcaaactgg
    16381 gaatgcagga tgatgggctg aatgatttgc tggtatcggt aaaaaaactg ctggcggaga
    16441 attatccgga tggtgtgttg cggccgggat tcgcctgagt aaacttccct catcgtgggg
    16501 cgtcagacgc cccctcaaac attaaaatgt gagcacttta tcggctgaca gcgtccattg
    16561 cgccagttcc acaagagtac cgatttccac cccatcaatc agaggaagtg tactaatccc
    16621 gcgcccgtcg gtacaggttt tgcacaattt caccggtaca ttctgagcgg taaggatctc
    16681 cagcatttgc tgaatgttgt agccttcccc tggtttttgc ccgcgcaacc cggctgtgac
    16741 cgcatcagac atgaggaaca gacgcagatc cagattgctc tcctgctctc gtaacgcaat
    16801 ggccagccgc aagctgttaa acaaggattc gctcccgtaa ggtgcgccat tggcaacgat
    16861 cacaattttt tgcattattt actcctgtat tcagggaatt agacactcat cttctatctt
    16921 actgcttctg cagcgtctga ccaatcggtc acatttttaa ggattttcct gaaagcgcga
    16981 gaaaatacga caaaagttgc cagtaatcgt tattctttaa ggctatggtt tttcattttt
    17041 taccggaagt taccgacgtt ttgagccgtt tcgttcctcg cattattccg ttttatttac
    17101 tcttgctggc ggcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa
    17161 atccctttga taataacaat gatggtctgc cggatttagg catggcacct gaaaatcatg
    17221 atggggaaaa acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata
    17281 acgggctgga tactggcgag caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc
    17341 ttagtcaaca ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagtg
    17401 ttgacgtcaa agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc
    17461 cgttacaaga taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggatg
    17521 atgggttggt gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg
    17581 gttataacac tttttatgac aacttgctgg acgaaaatct tcagcgagcg ggctttggtg
    17641 ccgaagcgtg gggcgaatat ttgcgactat cggcaaactt ttatcagcca tttgctgcat
    17701 ggcatgaaca gacagccacg caggaacaac ggatggcgcg cgggtacgac ctgacagccc
    17761 ggatgcgcat gccgttctat caacacctca ataccagtgt cagcgtagaa cagtattttg
    17821 gtgatcgtgt tgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc
    17881 tgggattaaa ttacacccct gtgccattag tcactgtgac ggcccagcat aaacagggtg
    17941 aaagtggcga gaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc
    18001 tcaaaaaaca actttctgcg ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct
    18061 atgataatcc gcagcgaaat aatctaccga ctcttgagta ccgacagcga aaaacgttaa
    18121 cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat
    18181 tacaaatccg cagtcgttac ggtattcggc aactgatttg gcagggcgat acgcagatat
    18241 taagtttgac gccgggcgca caagccaaca gcgcggaggg ctggacgctg atcatgcctg
    18301 actggcagaa cggggaaggg gcgagcaatc actggcgatt gtcggtggtg gtggaagata
    18361 accaggggca gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgacg
    18421 cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc
    18481 cataccgctg cttccacgcg agacttgagc ttcattttct tcagcatgtg cttgacgtgc
    18541 acttttactg tgctttcggt gatgtccagg cggcgggcaa tcatcttgtt cggcaaaccc
    18601 tgggcaatca gcttgagaat atcgcgctcg cggggggtta actggttaac atcgcgctca
    18661 gtcgtggcac ggttagcgcg caagctggcg gccagaacag gcgttaatgc ttcgcttaat
    18721 accatttcgc cagcagctgc ctgatgcaat gctttcagca gatcttccgg ttccatatct
    18781 tttaacagat agccatccgc gccgcgtttc agtgcggtga ccacatcttc ttcatggtta
    18841 gagacactga ataccacaat gcgccctgag agggactttt cgcgcagttt atccagcgtt
    18901 tccagaccgt tcatgccggg catattgaga tctaacagga tcagatcggg atcaagagat
    18961 tccgccagtt caataccctg ttcgccatta ctcgcttcgc caaccacggt gatatctggt
    19021 gccatactga taagctgttt tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg
    19081 atagtagccg gttcctgatt actcatgggt atctccttgg acgtctgtga aagttttttc
    19141 gggaataaag gtgaccacca cttcggtgcc acctgattca cgacggcgga cgcggcaatc
    19201 gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat
    19261 ggcattttca ggcacgccgc agccgttatc ctggacggtc agtttgacct gattatcgtt
    19321 ttgcgccacc gtcaccacga cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc
    19381 ctcacgggca atttgcaaca agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa
    19441 ttgataatcc agcttcaccg ggaagccaaa tttggcgctg tactcttcgc aactcgcctc
    19501 cagcgccgga cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg
    19561 caactgcgcc caggatgcat tcagttcgtt acggatctga cttaacagtt cgcggctgct
    19621 ttctggcagc gcatcgccct gcatctgtaa acaactcacc tgcatcttca tgcaagagag
    19681 agattgggca atagaatcat gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat
    19741 caactgttgc tgacgttcct gatgccgatc cagcgccagc gtggcggtga gttgttcaac
    19801 cagggtatcc accagttgtt gttgatcatg gctaagatga cgcccctgcg gcagggtcgc
    19861 cagcaaaata ccgtactgcg tatgagagtc agccagccgc cacttcaggg tcgtgccgcg
    19921 atcaccaacg ggtaatacgc cgcgcgggca gagctggcag cctttatcat cacaagtcat
    19981 atctggctgg caggtaaact cctgatgatt ctcttcatca tcagtgtcat acacccgcag
    20041 ttcgatatca cgtagcaggg ttaaattctg taagccgttg agtacaggtg acaggcgttc
    20101 acacagcggg gcgcgggaat gcaaacggcg gttagcctgc cataaaaaag agaggatctg
    20161 atttttatgc tccagcccgg cggttttctc ctgaacccgc tgctcaagta cggcataact
    20221 ttcggccagt tctgcagaca tattgttcaa cgcagttcca agcatcgcca tttcgttgcg
    20281 cccgctgatg tttgcgcgtt gggtaaaatc gcgatggctg acggcactcg ccattgccag
    20341 cagttgccgc cacggttgta gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa
    20401 aagtgccata aataccgcca ttacccgatg gaccagtacc actgtctcaa tacgcatttc
    20461 cgtggtgcgg tcaaaaccag ataccagctg atcaagcccg gcaacaaact ggctgacatc
    20521 cgctgatacc gtttctcggt tttgcgcccg catcagcgca gggatcagtt cattacgcca
    20581 gtaatcttgt aaaccctgta attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa
    20641 ctcggcgcta aatgccgttt gttccatctc tttaattaag ggcttgtctt tctcgcttaa
    20701 tggcactgct gccaacagac ggtaactttg catgcgcagc gatcccgctt tgttgatcgc
    20761 atgggcgctg ccctgaacgc cttgcaccag ccagccagaa accgccatcc ctgccagtcc
    20821 aatagcagta gaaagcaaca caataagcgc aacctgatta accagggtga gcggagagag
    20881 acaacgttta agcatgtaaa cctcttcctt caggctttaa atgagcaata accttaatga
    20941 atgtgacgat acattctgga atggcagtat tctcggctat tggctgaagt atacccatac
    21001 ccggaaagag ttactcctta tttgccgtgt ggttagtcgc tttacatcgg taagggtagg
    21061 gattctacag caccgtgaaa aatctcataa tttttatgaa atcactgtac tcactatggg
    21121 taatgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc
    21181 tttagctaca gacactaagg tggcagacat cgaaacgagt atcagaggtg tctatgagtc
    21241 actcatccgc ccccgaaagg gctactggag ctgtcattac agattggcga ccggaagatc
    21301 ctgcgttctg gcaacaacgc ggtcaacgta ttgccagccg caacctgtgg atttccgttc
    21361 cctgtctgct gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac
    21421 cgaaagtcgg ttttaatttt acgaccgatc agctatttat gttgactgcg ctgccttcgg
    21481 tttctggcgc gttattacgt gttccatact cctttatggt tcctatcttc ggtggtcgtc
    21541 gctggacggc gttcagcacc ggtattctga ttattccttg tgtctggctg ggttttgccg
    21601 tgcaggatac ctccacgcct tatagcgtct tcatcatcat ctctctgctg tgcggctttg
    21661 ctggcgcgaa cttcgcttcc agtatggcaa acatcagctt cttctttccg aaacagaagc
    21721 agggtggcgc gctgggcctg aatggtggtc tgggcaacat gggcgttagc gttatgcagt
    21781 tggttgctcc gctggtggta tcactgtcga ttttcgcagt atttggtagc cagggcgtca
    21841 aacagccgga tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc
    21901 ttgccatctt caccattgcg gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct
    21961 ccatcaagga gcagttgccg gtactcaaac ggggtcatct gtggattatg agcctgctgt
    22021 atctggcaac cttcggctcc tttatcggct tctccgcggg ctttgcgatg ctgtcaaaaa
    22081 cgcagttccc ggatgttcag attctgcaat acgctttctt cgggccgttt attggtgcgc
    22141 tggcgcgttc tgcaggtggt gcattatctg accgtctggg cggaactcgt gtcacgctgg
    22201 tgaactttat tctgatggcg attttcagcg gcctgctgtt cctgacctta ccgactgacg
    22261 ggcagggcgg aagcttcatg gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg
    22321 ggctgggtag tggttccact ttccagatga tttcagtgat cttccgtaaa ctgacaatgg
    22381 atcgcgtgaa agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca
    22441 cggcggcggc gctgggtttc atctctgcga ttggcgcgat tggtggcttc tttatcccga
    22501 aagcgtttgg tagctcgctg gcattaacgg gttcgccagt cggcgcaatg aaggtatttt
    22561 tgattttcta tatcgcctgc gtagtgatta cctgggcggt atatggtcgg cattctaaaa
    22621 aataaatctt aatatttgat tatcctttgc gcggcatgat gtcgcgcttt ttttatgcgt
    22681 catttagtta caacatacta atgttatatg gtttatttcg ccggatttca ttaagagcca
    22741 ttaata
//
LOCUS       DAZGBC010000077        22420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGBC010000077 DAZGBC010000000
VERSION     DAZGBC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.077"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..150)
                     /locus_tag="ACHK1J_002804"
     CDS             complement(<1..150)
                     /locus_tag="ACHK1J_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912048.1"
                     /translation="MPRTCILYAHPSTTAGCGVNALSGLRMAREFVGLIRRVSVASGI
                     CARLPD"
     gene            complement(157..1170)
                     /gene="mhpE"
                     /locus_tag="ACHK1J_002805"
     CDS             complement(157..1170)
                     /gene="mhpE"
                     /locus_tag="ACHK1J_002805"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO7912049.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(1167..2117)
                     /gene="mhpF"
                     /locus_tag="ACHK1J_002806"
     CDS             complement(1167..2117)
                     /gene="mhpF"
                     /locus_tag="ACHK1J_002806"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO7912050.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLDANVEQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(2114..2923)
                     /gene="mhpD"
                     /locus_tag="ACHK1J_002807"
     CDS             complement(2114..2923)
                     /gene="mhpD"
                     /locus_tag="ACHK1J_002807"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO7912051.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(2933..3799)
                     /gene="mhpC"
                     /locus_tag="ACHK1J_002808"
     CDS             complement(2933..3799)
                     /gene="mhpC"
                     /locus_tag="ACHK1J_002808"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO7912052.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            complement(3817..4761)
                     /gene="mhpB"
                     /locus_tag="ACHK1J_002809"
     CDS             complement(3817..4761)
                     /gene="mhpB"
                     /locus_tag="ACHK1J_002809"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO7912053.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(4763..6427)
                     /gene="mhpA"
                     /locus_tag="ACHK1J_002810"
     CDS             complement(4763..6427)
                     /gene="mhpA"
                     /locus_tag="ACHK1J_002810"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO7912054.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            6618..7451
                     /gene="mhpR"
                     /locus_tag="ACHK1J_002811"
     CDS             6618..7451
                     /gene="mhpR"
                     /locus_tag="ACHK1J_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO7912055.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            7566..8342
                     /locus_tag="ACHK1J_002812"
     CDS             7566..8342
                     /locus_tag="ACHK1J_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO7912056.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEVTMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIVA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            8362..9315
                     /locus_tag="ACHK1J_002813"
     CDS             8362..9315
                     /locus_tag="ACHK1J_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO7912057.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKHFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            9352..10434
                     /gene="lacI"
                     /locus_tag="ACHK1J_002814"
     CDS             9352..10434
                     /gene="lacI"
                     /locus_tag="ACHK1J_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO7912058.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEYLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            10557..13631
                     /gene="lacZ"
                     /locus_tag="ACHK1J_002815"
     CDS             10557..13631
                     /gene="lacZ"
                     /locus_tag="ACHK1J_002815"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO7912059.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFSAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLRGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGRGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLPDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            13683..14936
                     /gene="lacY"
                     /locus_tag="ACHK1J_002816"
     CDS             13683..14936
                     /gene="lacY"
                     /locus_tag="ACHK1J_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO7912060.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            15002..15613
                     /gene="lacA"
                     /locus_tag="ACHK1J_002817"
     CDS             15002..15613
                     /gene="lacA"
                     /locus_tag="ACHK1J_002817"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO7912061.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSDV
                     EKRESLIKEMFATVGENAWVEPPVYFSCGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(15716..16897)
                     /gene="cynX"
                     /locus_tag="ACHK1J_002818"
     CDS             complement(15716..16897)
                     /gene="cynX"
                     /locus_tag="ACHK1J_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO7912062.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTQVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLLLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(16903..17373)
                     /gene="cynS"
                     /locus_tag="ACHK1J_002819"
     CDS             complement(16903..17373)
                     /gene="cynS"
                     /locus_tag="ACHK1J_002819"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO7912063.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(17404..18063)
                     /gene="cynT"
                     /locus_tag="ACHK1J_002820"
     CDS             complement(17404..18063)
                     /gene="cynT"
                     /locus_tag="ACHK1J_002820"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO7912064.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            18173..19072
                     /gene="cynR"
                     /locus_tag="ACHK1J_002821"
     CDS             18173..19072
                     /gene="cynR"
                     /locus_tag="ACHK1J_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO7912065.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHTALEECADVGENESR"
     gene            complement(19412..20695)
                     /gene="codA"
                     /locus_tag="ACHK1J_002822"
     CDS             complement(19412..20695)
                     /gene="codA"
                     /locus_tag="ACHK1J_002822"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO7912066.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKICAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(20685..21944)
                     /gene="codB"
                     /locus_tag="ACHK1J_002823"
     CDS             complement(20685..21944)
                     /gene="codB"
                     /locus_tag="ACHK1J_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO7912067.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGLADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         5280 a         6205 c         5909 g         5026 t
ORIGIN      
        1 atccggcagt cgtgcgcaga tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa
       61 ttctcgcgcc atccgtaggc cggataaggc gttcacgccg catccggcag tcgtgctggg
      121 atgggcatac aaaatgcacg ttctcggcag tcaatattat ttgttgttgc gcagatccag
      181 cgccacgtcg acgatcatat cctcctggcc gccaaccatc cggcgtttgc ccagctcaac
      241 gagaatatcc accgcgctta agccataacg cgccgccgcc gtttcacagt gacgcaggaa
      301 gctggagtaa acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg
      361 atcctgcaac ggacgcacca ggtcgtcagc ggcatccatt aacgcataga gatcggcccc
      421 atgctgccaa cccagtttat ccacggcggc aataaatact tccagcggcg cgttacctgc
      481 gcccgctccc attcccgcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc
      541 gatagagttc gccacgccaa gactcaggtt atggtgagcg tgcatgccgg tttgcgtttc
      601 tggtttcagc actgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc
      661 accgccagaa tccaccacat aaatgcaggt cgcaccgtag ccttccatca gctttgcctg
      721 cttggcgaga ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat
      781 tccgagctcg cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt
      841 agccacacga accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc
      901 gattcctggc agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgctgcttc
      961 aatccattca aggtcgctat gtgcgccgaa accatagtta aagctggacc cctgcaaacc
     1021 gtcgccgtgg gccacttcaa tcgaatccac gcgggcatcg tccagtgctt tggcaatctg
     1081 gcgaacgttt tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaacgtgac
     1141 gtccgatata taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac
     1201 tgggccattt tttccgctgt cgccagcgca ctggaagtca taatgtcgag gttgcccgca
     1261 taggcaggca gataatgcgc tgcgccttcg acttccagcc agaccgctgt tttcagtccg
     1321 gagaattgcc ccacgcccgg taaattgacc ggtttatcct gcgggataac ttcaaactgc
     1381 acgcgctgtt tcaggcgata tcccggtacg taagcctgca ccgcctcagc catttcattg
     1441 attgaggctt cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc
     1501 atcatcaacg gtggctctgc tgggttaagc acgataattg ccttcccttt tgccgcgccg
     1561 cccaccactt caatggctcg ggaagtggtt tccgtaaatt catcgatatt ggcacgcgtg
     1621 ccaggtccgg cagatttact ggcgatagaa gcgataattt cggcgtaatg aacacgcgcc
     1681 acgcgtgaaa ctgccgccac cattggaatg gtggcctggc cgccgcaggt gaccatattg
     1741 acgttcagtt gttcgacatt cgcatcaagg ttaaccaccg gcacgcagta agggccgatg
     1801 gcagcaggcg tcaggtcgat taagcgaata tccggtttcg cttcgcgcaa agcggcatcg
     1861 tttttcacat gtgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca
     1921 ggcatgttca tcagcccgat caccccttca tgggtggtgg cgacgcccat acgtctggca
     1981 cgcgccagac cgtcggactg aggatcaata ccgaccatca ccgccatctc cagatgctga
     2041 ccgtgacgca aaattttaat catcagatcg gtgccaatgt tgccagaacc gataatggcg
     2101 actttacgct tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa
     2161 ctgaacctat gccttcaata tgggcttcaa aacgatcgcc cgcattcact gccaccatcg
     2221 gacctaatgc cccggtaaga atgatatctc cggcgcgcag cggttcaccc agactggcca
     2281 ttttgcgtgc cagccagacg gcggcattaa gcggatgtcc caggcattcg ctgccgcgcc
     2341 cgctggaaac ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc
     2401 ccgccggacg ttgcgccgga ccgccgatga catacacccc gcaggaagcg ttatctgcca
     2461 cggtatcgac aaactgaatc gaccagtcgc gaatgcggct acccaccacc tccagcgccg
     2521 gaagtaccca ttcaatggcg ttatacaatt cgtcgaaggt gatatcggtt gcgggcaaat
     2581 cgcggttcaa caccagtgcg atctccgctt caatgcgggg ttgcagaaca cgggaaaaag
     2641 gaatgatttc gttatcgcca taacacatgt cggcaaataa tgtcccgaaa tccggttgat
     2701 caacgcccag ttgttgctgc actttcggat gtgtcaggcc cactttacgc cccaccacgc
     2761 gacgcccctg cgcaacgtca tattgcacat ttatgtgctg aatggcgtag gccgcttcag
     2821 cgttatcgat accaatcaga tcgcgcagcg gcgcaatcgc ttcgccctgc tctgcggcgc
     2881 ggcgtaaatc cgccgccagt tgctcaagag tatgcttcgt catgaccgtt ccttaagcac
     2941 gtgcgaggaa attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccaat
     3001 gaccacagtc gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac
     3061 ccgcatccat cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga
     3121 tttccgccag acgtgggcca aaatccggga actgtttcgg attagcttcc aggctcttaa
     3181 cgaagttttc caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg
     3241 cgtcggtcaa atcgctggta tcaaaaacga agatatccat catcagcttc aggttttcga
     3301 tagtcggctg acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa
     3361 acaaactcat gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc
     3421 agttaagggt gaacgccaca gaactatggc cacccatcga gttgcccagc aggtggattt
     3481 tggcgatatc cagttgatcc accacgcttt tcaggattcg tgcattaaga tccgatcgcg
     3541 aaccactatt aacgatcgaa tcgctcttgc cccaacccgg acaatccagc aggatcaccc
     3601 gatagcccgc ctctaccagc ggatcgatat tgcggctgaa gttcgcccag ccagtagcac
     3661 ccgggccgga accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat
     3721 ggatgcgcag cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg
     3781 tttgtggctg ataactcata atcttctcct gcatattcag ttctctgttc tggcgcttaa
     3841 cgagccaaat ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca
     3901 gttgccaaat gcagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga
     3961 ctttccggca atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc
     4021 ctgctccagc aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagagttct
     4081 ctgatcctca acaaacttct cagcggcgct aatcaccctt tgctgacgca attcgcgctc
     4141 actggcgggt aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt
     4201 cgccagttcg ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg
     4261 tttattgaga gtgctggtga aacgtccaat ggcttcaccc aacatgcggg tacgctggaa
     4321 accgggcagc ggcgtggcga caccgttgat gaacacaggc agaactggca ccttatccag
     4381 cccaccgagc aggaactcca gcggctgtgc gaacccgtgg tccacctgca tacagtaaga
     4441 aacggcaaga tcgatcccgc tcttcatgac ggcatgcgca caggcctccg ccagctccac
     4501 aggcactggc agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc
     4561 taaacagaac ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag
     4621 caccaccagt tcaggggaga aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt
     4681 gacctcatcg agtacctctt gtgctgggtc gacatacccc accagcggcg agtgggaaag
     4741 acaatgaaga taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc
     4801 gggtcagcgt catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg
     4861 cggtggcggc aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgcgcga
     4921 accagctacg caggcgacct tgcgtatccc ccacgcgtag tacgccgtcg tggttatcct
     4981 gtgcggtatg aatttgcact tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc
     5041 actgctggat ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg
     5101 cgaagttcgc gccgatcgcg ttatcgagta gcgtcacgtc gccgttttcc agcgtgactt
     5161 tcggctgaat aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgtaccagcg
     5221 caccgccgta atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg
     5281 gcggcagata attcagcagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg
     5341 gcggagccag cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc
     5401 gacgttcttg ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg
     5461 ccagtttcca tgcgaggtta aaagcgtcgc gcatgccact gttatagccc tgcccctgcc
     5521 ataccggcat gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt
     5581 gcgccagtcg cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat
     5641 taggcagcac tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgttcttcgg
     5701 tttctcctgg catcaccata aattcaaagc gacgtaccgc atgaggtagc gcggcagaaa
     5761 catacgggcg caccggatcg caacacaaat agatatgcgg cgtacttaac ggatcgttgg
     5821 cgatatctac cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcaggg
     5881 tgcgacggac aaaacttgcc ccaccatcac aggccaccag ccactgggct ttgactattt
     5941 cccgctgccc ttctgccgtt ttcaggtgca aggtcacttc gtcatcttgc tgactgaagg
     6001 cctccagctc gcgggaaaac aagcagcgca cattcggaaa acgcgacacc ccttccagca
     6061 tcaccgcatc gacctgcggc tgaataaagg cgttacggcg cggccagcca aattcatcgg
     6121 tcattggctg aatatcagca aaacagcggc ccttcggggt gagaaaacgc atcgcgtgcc
     6181 acggcgtagt atgcggcaga acattatcga ccaggccgac cgactgcatg gtgcgcagcg
     6241 cctcgtcatc aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca
     6301 ccagcacgtc aatgcccatc tggccgagat agttcgccat catcagcccg accggaccgg
     6361 caccagcgat cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta
     6421 ttgccatttc agtacctcac gactcggaca aaatgccgtt gcgcgcacag tacagcgcaa
     6481 cttattttgt taaaaacatg taaatgattt tttattgtgc gctcagtata gaaagggtgt
     6541 tttcggctac aatcaaaaca tgcccgaatg tgcaccaggt gcacaacgtt gttttaacta
     6601 tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct
     6661 taacccgcgg tctaatgtta ttaaatatgt taaataaact tgacggcggt gccagcgtcg
     6721 ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc
     6781 tgcaggaaga gggatatgtc cggcgtagtc cctccgatga tagttttcgt ctgaccatca
     6841 aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc
     6901 cactgctggg cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc acgctggatg
     6961 ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc tttcaccggg
     7021 caatggtcgg gcgacgtttg ccgcttctga aaaccgcctc gggcctgacc tggctggcct
     7081 tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agcctcccgc cccggtgatg
     7141 actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgt gcgcgcaaag
     7201 agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg
     7261 taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg
     7321 caatgaccat cgaacaggca gcggaaaagc atcttccggc actacaacgg gtagcaaaac
     7381 agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga agcggcgtgc
     7441 atttacgcta attacgccaa tgtgtaattt ggcccattct catcgttatt cgcccatttg
     7501 cccacatgca cagtacgggc tgcacgctac gctaatgaca tcaatactgt gatgtggagc
     7561 aaatcatgaa aattacgcaa attcgtaatg ccacccaaat tatccaatac gctggcaaaa
     7621 catttcttat cgacccgtta ctcgcgccta aaggaagtta tccgggtttt gccggaacgg
     7681 cacgcgcaga aatccgcaat ccaacggtag aattaccgtg cgatttaaca acgttactca
     7741 acgttgacgc cctaatcgta acccatttgc atgaagatca ctgggacgaa acagcctccc
     7801 gcgtcgtccc taaagataaa ctgatttatg tgcaaaatga gcaagatgct gaatcactga
     7861 ataaacaagg atttacgcat cttaccgtac ttaccgacga gactaaattt ggcgatatta
     7921 ctctgcataa aacgacaggg cagcacggct ccgatcgcac ctatgccgac ccggctatgg
     7981 cgaagcgttt aggcgcagcc tgcggcgttg ttttccagca cccgacagag aaaacgctct
     8041 atctggctgg cgataccctc tggcgtgacg acgtcgaggt tacaatgcta actttccaac
     8101 cagaagtggt tgtgttgaat gccggatttg cgcatgttct ggggcatggc gcgataatta
     8161 tgggagcaga agatattctc aaaacacatt ttaccctgcc agaagcgcaa attgtcgcga
     8221 cacatatgga ggccgtaaac cactgtttgc tgacgcgtga tgcgctcaag gaatacgcca
     8281 gagataatca aattatcgaa tttgtgaacg taccagaaga tggtgaaacg ttgacattct
     8341 aattcggaga taaagagtgt tatgcgcccg ctaactgttg ccattatcgc cgttgccggt
     8401 tttagtcctt ttcacctttc cgtaccgttt atcgtattta gcgaaaaaat ggcggagaaa
     8461 aaacactttc acgtaattat ctgcgctgaa aagccgggaa acgtggactc tgccgatggg
     8521 ttttccgtaa ccgccaccca tgactatacc gcagtcatcc aggcggatat tgtgataatt
     8581 ccttactggg gaaccattac acaaaaaccg ccacaaaaac tgctggaagc cttaacgacc
     8641 gcacgggata acggcgcaca aattgtcggg ctttgcctgg gcacgtttgt gctcggctat
     8701 gcaggtttac tgaaaaataa gcgtgccgcc acgcactggg agttcgagcg tgaatttcag
     8761 gcacgttttc cacaaacaca tctggatatt aacgcgttgt acgtagacga tgacggcatt
     8821 attacctctg ccggtactgc cgcggcgctg gattgctgtt tgtatattgt tcggcaacat
     8881 tttggcagcg actatgctaa ccatattgcc cgacggatgg tcgtaccgcc atatcgcacc
     8941 ggcggtcagg cgcagtttat tgagcaacca gtgccgaaaa atacccatga tgaacgcatt
     9001 aatctcctgc tggattacct gcggcaaaac attgcgcaac agcatgatct cgactcgctg
     9061 gcgcagcgag taatgatgag tcgccgcaca ttaactcgcc attttatgaa agcgaccggt
     9121 tcgagtatcg ccgaatggct cattactgaa cgcttacgcc gtagccagga actgttggga
     9181 tccagtcagt tgcccgttga gcggatagcg gctgaggtgg gttttctctc acctgtgacc
     9241 tggcgtcagc attttaaatc tcacttcggc gtcagccccg ccgaatggcg caaaaccttt
     9301 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
     9361 gtaacgctat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     9421 gtgaatcagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     9481 gagctgaatt acattcccaa ccgcgtggca caacaactgg cggggaaaca gtcgttgctg
     9541 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     9601 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     9661 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     9721 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cgctaatgtt
     9781 ccggcgttat ttcttgatgt ctctgaccag actcccatca acagtattat tttctcccat
     9841 gaagacggta cgcgactggg cgtggagtat ctggtcgcat tgggtcacca gcaaatcgcg
     9901 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggcggg ctggcataaa
     9961 tatctcactc gcaatcaaat tcagccgata gcggaacggg agggcgactg gagtgccatg
    10021 tccggttttc aacaaaccat gcaaatgcta aatgagggca tcgttcccac tgcgatgctg
    10081 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggttgcgc
    10141 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc gtgttatatc
    10201 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
    10261 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
    10321 gtgaaaagaa aaaccaccct ggcgcccaag acgcaaaccg cttctccccg cgcgttggcc
    10381 gattcattaa tgcagctggc acgacaagtt tcccgactgg aaagcgggca gtgagcgcaa
    10441 cgcaattaat gtgagtcagc tcactcatta ggcaccccag gctttacact ctatgcgtcc
    10501 ggctcgtatg ttgtgtgaaa ttgtgagcgg ataacaattt cacacaggat acagctatga
    10561 ctatgattac ggattcactg gccgtcgtat tacaacgtcg tgactgggaa aaccctggcg
    10621 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    10681 aggcccgcac caatcgccct tcccaacagt tgcgcagcct gaatggtgag tggcaatttg
    10741 tctggttttc ggcaccagaa gcggttccgg aaagctggct ggagtgcgat cttcctgtcg
    10801 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgacgcg cccatctaca
    10861 ccaacgtgac ctatcccatt acggtcaatc cgccatttgt tcccacggag aatccgacgg
    10921 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa
    10981 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt
    11041 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag
    11101 aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg
    11161 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcacaaa ccgaccacac
    11221 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gcggttctgg
    11281 aggcagaagt tcagatgtac ggcgagctgc gcgatgagct gcgggtgacg gtttctttgt
    11341 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg
    11401 agcgtggcgg ttatgccgat cgcgtcacac taggtctgaa cgtcgaaaac ccgaaactgt
    11461 ggagcgccga aatcccgaat atctatcgtg cggtggttga actgcacacc gccgacggca
    11521 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtacggatt gaaaatggcc
    11581 tgctgctgct gaacggcaag ccgttgctga ttcgcggcgt taaccgtcac gagcatcatc
    11641 ctctgcgtgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc
    11701 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacaccc
    11761 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg
    11821 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc cgcgatgagc gaacgcgtaa
    11881 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg
    11941 agtcaggcca cggcgctaat cacgacgcac tctatcgctg gattaaatct gtcgatccat
    12001 cccgcccggt gcagtatgaa ggcggcggag ccgacacctc cgcaaccgat attatttgcc
    12061 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca
    12121 aaaaatggct ttcgttgcct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg
    12181 cgatgggtaa cagtcttggc ggcttcgcta aatactggca ggcgtttcgt cagtaccccc
    12241 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa
    12301 atggcaatcc gtggtcggct tacggcggtg attttggcga tacgccgaat gatcgccagt
    12361 tctgcatgaa cggtctggtc tttgccgacc gcacgccgca tccggcgctg acggaagcaa
    12421 aacaccagca gcagtttttc cagttccgtt tatccgggcg aaccatcgaa gtgaccagcg
    12481 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggca
    12541 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa caggtaattg
    12601 aactgcctga actaccgcag ccggagagca ccgggcaact ctggctaacg gtacacgtag
    12661 tgcaaccgaa cgcgaccgca tggtcagaag ccggacacat cagcgcctgg cagcagtggc
    12721 gtctggcgga aaacctcagc gtgacactcc cctccgcgcc ccacgccatc ccgcatctga
    12781 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc
    12841 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc
    12901 gcgatcagtt cacccgcgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca
    12961 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcgg
    13021 cgttgttgca gtgcacggca gatacacttg ccgacgcggt gctgattacg accgctcacg
    13081 cgtggcagca tcagggaaaa accttattta tcagccggaa aacctaccgg attgatggta
    13141 gaggtcaaat ggcgattacc gttgatgttg aagtagcgag cgatacaccg catccggcac
    13201 ggattggcct gacctgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag
    13261 ggccgcaaga aaactatccc gaccgcctta ctgctgcctg ttttgaccgc tgggatctgc
    13321 cattgccaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga
    13381 cacgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc
    13441 gctacagcca acaacaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag
    13501 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc
    13561 cgtcagtgtc ggcggaattc cagcttagcg ccggtcgcta ccattaccag ttggtctggt
    13621 gccaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca
    13681 ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact
    13741 tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata
    13801 tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc
    13861 aaccgctgtt tggtctgctt tctgacaaac tcgggctgcg caaatacctg ctgtggatta
    13921 ttaccggcat gttagtgatg tttgcgccgt tctttatttt tatcttcggg ccactgttac
    13981 aatacaacat tttagtagga tcgattgttg gtggtattta tctaggcttt tgttttaacg
    14041 ccggtgcgcc cgcagtagag gcatttatcg agaaagtcag ccgtcgcagt aatttcgaat
    14101 ttggtcgcgc gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca
    14161 tcatgttcac catcaataat cagtttgttt tctggctggg ttctggctgt gcactcatcc
    14221 tcgccgtttt actctttttc gccaaaacgg atgcgccctc ttctgccacg gttgccaatg
    14281 cggtaggtgc caaccattcg gcatttagcc ttaagctggc gctggaactg ttcagacagc
    14341 caaaactgtg gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg
    14401 accaacagtt tgctaatttc tttacttcgt tctttgctac cggtgaacag ggtacgcggg
    14461 tatttggcta cgtaacgaca atgggcgaat tacttaacgc ctcgattatg ttctttgcgc
    14521 cactgatcat taatcgcatc ggtgggaaaa acgccctgct gctggctggc actattatgt
    14581 ctgtacgtat tattggctca tcgttcgcca cctcagcgct ggaagtagtt attctgaaaa
    14641 cgctgcatat gtttgaagta ccgttcctgc tggtaggctg ctttaaatat attaccagcc
    14701 agtttgaagt gcgtttttca gcgacgattt atctggtctg tttctgcttc tttaagcaac
    14761 tggcgatgat ttttatgtct gtactggcgg gcaatatgta tgaaagcatc ggtttccagg
    14821 gcgcttatct ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc
    14881 ttagcggccc cggcccgctt tccctgctgc gtcgtcaggt aaatgaagtc gcttaagcaa
    14941 tcaatgtcgg atgcggcgcg agcgccttat ccgaccaaca tatcaggacg gaatgagcga
    15001 aatgaacatg tcgatgacag aaagaataaa agcaggcaag ctatttaccg atatgtgcga
    15061 aggcttacct gaaaaaagac ttcgtgggaa aacattaatg tatgagttta atcactcgca
    15121 tccatccgac gttgaaaaaa gagaaagcct gattaaagaa atgtttgcca cggtagggga
    15181 aaacgcctgg gtagagcccc ccgtctattt ctcttgcggt tccaacatcc atataggccg
    15241 caatttttat gcaaatttca atttaaccat tgtcgatgac tacacggtaa caatcggtga
    15301 taacgtactg attgcaccca acgttactct ttccgttacg ggacaccctg tacaccatga
    15361 attgagaaaa aacggcgaga tgtactcttt tccgataacg attggcaata acgtctggat
    15421 cggaagtcat gtggtcatta atccaggcgt caccatcggg gataattctg ttattggcgc
    15481 gggtagtgtc gtcataaaag acattccacc aaacgtcgtg gctgctggcg ttccttgccg
    15541 ggttattcgc gaaataaacg accgggataa gcaatattat ttcagagatt acaaagttga
    15601 atcgtcagtt taaattagca aaattgcctg atgcgcttcg cttatcaggc ctacaaattc
    15661 agtgttctac gttagccgca tccggcatga acaaagcgca ggaacaagcg tcgcatcatg
    15721 cctctttgac ccacagctgc ggaaaacgtg ctggtgcaaa acgcagggtt atgatcatca
    15781 gcccaacgac gcacagcgca tgaaatgccc agtccatcag gtaattgccg ctgatactac
    15841 gcagcacacc agaaaaccac ggggcaagcc cggcgatgat aaaaccgatc ccctgcataa
    15901 acgccaccag cttgccagca atagccggtt gcgcagagtg atcgagcgcc agcagcaaac
    15961 agagcggaaa cgcgccgccc agacctaacc cacacaccat cgcccacaat accggcaatt
    16021 gcagcggcag ccagataaag ccgcagaaac ccaccagttg taacgccagt gccaacagca
    16081 aaagtttgcg ccgatcctga tggcgagcca tagcaggcat tagcaaagct cctgcggctt
    16141 gcccaagcgt catcaatgcc agcaaggaac cgctgtactg cgcgctggca ccaatctcaa
    16201 tatagaaagc gggtaaccag gcaatcaggc tggcgtaacc accgttaatc agaccgaagt
    16261 aaacacccag cgtccacgcg cgaggagtga aaaccacgcg aacctgagtg gttgttgtct
    16321 tgtgggaaga ggcgacctcg cgggtgcttt gccaccacca ggcaaagagc gcaacaacgg
    16381 caggcagcgc ccaccaggcg agtgtttgat accaggtttc gctatgttga actaaccagg
    16441 gcgtcagggc ggcaccaagt ccaccgccgc ccatcagagc cgcggaccac agccccatca
    16501 ccagtggcgt gcgctgctga aaccgccgtt taatcaccga aggcatcacc gcctgaatga
    16561 tgccgatccc caccccacca agcagtgcgc tgctaagcag cagcgcactt tgggggtaaa
    16621 gctcacgcat caatgcaccg acggcaatca gtaacaaact gatggcgacg ctgcgacgtt
    16681 cgctgacatg ctgatgaagc cagcttccgg ccagcgccag cccgcccatg gtgaccaccg
    16741 gcagagcggt caacagggca gccacgctaa agctcattcc gcttgcctgg cgcaattgcg
    16801 gtagcagtgg cccgacggag gtgagcagtg gtcgcatatt aagaccaatc agcaccagaa
    16861 ccagcagcat cgtgaacgtc ttttgatggt tacgcatggc tgtcagaacg gtttggtcgg
    16921 cagatattta ccatctaagg tgatgaccgc acgttcgcca ccttccgggt ccgccacttt
    16981 cttaacgtcg agtttgaagt taatcgcgct gataatgcca tcgccaaact tctcatgaac
    17041 caacgctttc agggttgtgc cgtacacctg caacatttca tagaaacgat acatcgttgg
    17101 gtcggttgga atacggtcat caatgcagcc acgcagtggg atcatctgta acagtagaat
    17161 ggcgtcttcg tcgagatcca gcttcgcccc gacctggcgg gcggcgtcgg caggaagcgc
    17221 ctgctgaccc agcaaagccg cggtcacaaa ggcttctgcc agcccggtgc cgtcggcaat
    17281 ctcggcaaat gagagatctt ttttagcttt gctgagcaaa atggcatcgg caagatcaag
    17341 acgaatattg cgattaattt gcgactgaat catggtgcat ctcctgatgg tttaaaaata
    17401 aggttacgct gcggtcggtt ggcgtagcgg tatggcacaa acgcgaggat tagcggccag
    17461 tggcacaaac tggcgggttg cgccgtcaaa cgctgcgatg ctgccgcttt caatgtcgta
    17521 gacccagccg tgcagggcga tccgcccctc ttcgagcgcc aggcgcaccg atggatgagt
    17581 ttgcaaatta gccaactgag caatgacgtt ttcacgtacc atcgccgcag cttttgacgg
    17641 taaatcggaa tgcgggcgcg cctcattaac gacgcgggct gaatcggcat aacgcagcca
    17701 gtgggagacg gcaggcatat ggtccatgca ctgacagctg gcaatggcgg tcatcgcgcc
    17761 acagttggaa tgaccgcaaa tcacaatgtc agatacccga agcgcagcga cggcatactc
    17821 caccgaagca gaaacgccac cgggttccgg cccgtaggaa gggacgatat tgcccgcgtt
    17881 gcgaataacg aacagatcgc caggctcacg ttgcgtcacc agctcaggga ccagacggct
    17941 gtcggagcag gagataaaaa gtgtgcgtgg gctttgctgt gtcgccaact gtttaaataa
    18001 ggcttcccgc ttcggaaatg cctcgcgctg gaatttaagg aatccatcaa taatctcttt
    18061 caccgttaac ctctgtctgt ctctggaatg agaggcagac tactcgccga ttgtcataag
    18121 gtaaaagtct catttatgat gagttctatt ggatttactt ataggttggc gaatgctctc
    18181 tcgacatatc aattattttc ttgccgtggc tgaacatggc agcttcaccc gtgccgccag
    18241 tgcgttgcac gtctctcagc ctgcgctttc ccagcagatt cgccagttag aggagagttt
    18301 aggcgtgccg ctgtttgacc gtagcgggcg aacgattcgt ctcactgatg caggagaagt
    18361 ctggcgacag tacgccagcc gggcgttaca ggaactggga gcgggtaaac gggcgattca
    18421 tgatgttgcc gatctgacga gaggatcgct gcgtatcgcc gtcaccccca cctttacgag
    18481 ctactttatc ggccccttaa tggcggattt ctatgcgcgc taccccggca tcacgctcca
    18541 gctacaggaa atgtcgcagg agaaaatcga ggatatgctt tgccgcgacg agttggacgt
    18601 tgggattgcc ttcgcgcctg tgcattcgcc ggagctggag gcaattcctt tactgacaga
    18661 aagtttagcg ttagtcgtgg cgcaacatca tccgctggcc gtccatgaac aggtggcgtt
    18721 gagtcgcttg catgatgaaa aactggtcct gctcagcgcg gagtttgcca ccagagagca
    18781 aattgaccac tactgcgaga aagcggggct gcatccacag gtggtcattg aggcgaactc
    18841 aattagcgcg gttctggagc tgattcgccg cacttccctt tccacattgt taccagcagc
    18901 gattgccaca caacatgacg ggcttaaagc tatttctctt gccccgccac tactggagag
    18961 aacggcggtt ttgttgcggc ggaaaaatag ctggcagaca gccgccgcga aggcattttt
    19021 gcacacggcg ttggaggaat gtgcggatgt tggcgagaat gaatcacggt agccactggc
    19081 acaggtctgc ggctggaatt gcctgatgcg ctacgcttat caggcctacg agttcagtgt
    19141 aaggcgttca cgccgcatcc atcataaact acagctggca gacagccgcc gcgaaatcat
    19201 ttttgcacat ggcattggaa gaatgcgcgg atgttggcgg gaatgaatca cggtagccgc
    19261 tgacacagat cagcggctgg aattgcctga tgcgctacgc ttatcaggcc tacgagttct
    19321 acgagttcag tgctctacgt aggccggata aggcgttcac gccgcatccg gcataaacta
    19381 cgctcgcttt gtcaacacac agcccagccg atcaccgttt gtaatcgatg gcttctggct
    19441 gctccagata tacggtggtt tgtgccggtt gtgtgctggc aatcaccttg ccgccacgta
    19501 ccgaataacg taccggaacc tgacggcgca gcgcatcaaa tccattttca gccggcagga
    19561 taatcaggtt ggcgctgttt ccggcggcaa tgccgtaatc ctgcaaattc aacgtcctgg
    19621 cgctgtggtg ggtgattaaa ttcaggccat cgttaatctg cccatagccc atcagctggc
    19681 aaacatgcag ccccatatgc agcacttgca gcatattcgc cgttcccagc ggataccacg
    19741 gatcgaagac atcatcgtga ccaaagcaga cgttaatgcc ggactccagc atctctttaa
    19801 cgcgcgtgat gccgcgacgt tttggatacg tatcgaaacg tccttgcaga tgaatattga
    19861 ccagcgggtt ggcgacaaag ttaataccgg acattttcag caagcggaac aggcgtgagg
    19921 tatacgcccc gttataggag tgcattgccg tggtgtggct ggcggtgact cgcgcgccca
    19981 tgccttcacg gtgcgccagg gcagcaacgg tttcgacaaa gcgcgactgc tcgtcatcga
    20041 tctcatcaca gtgaacgtcg atgagacggt cgtatttttg cgccaaggcg aaggttttat
    20101 gcagcgactc cacgccgtat tcacgggtaa attcaaaatg cggaatcgcc cccactacat
    20161 ctgcccctaa gcgtaacgcc tcttccagca acgcttcacc gttgggatac gacaaaatcc
    20221 cttcctgagg gaaggcgacg atttgcagat caatccacgg cgcgacttcc tgcttcactt
    20281 ccagcattgc tttcagcgca gttagcgttg catccgaaac atcgacatgg gtacgcacat
    20341 gctgaatgcc gttggcaatc tgccatttca gcgtttgcca tgcgcgttgt ttcacatcgt
    20401 catgggttaa taacgctttg cgctcggccc agcgttcaat gccttcaaac agcgtgccgg
    20461 actgattcca gttcggttgt ccggcggttt gcgtggtgtc caggtgaata tgtggctcca
    20521 caaacggcgg tataactaaa ccttgttcgg catccaggct gttttcagtt atgggcatca
    20581 cgccggattg cgcatcaatg gcgcagattt ttccgtcctg cagatgaatc tgccacagcc
    20641 cctctttgcc tggtaaccgg gcgttaataa ttgtttgtaa agcgttattc gacactgtta
    20701 gcctccacat gcgtcattgc tgctgtcgtt ttacgattca aaatcgggtt aaggatcaga
    20761 tagctcagcg cgccacctaa taccgcgttg accggaacaa ttcccggtaa ccagtggcct
    20821 gcagcaatcc ccagggcgac cgccagaatc gccacccaat tgacactcat catacgcgtg
    20881 gtcgcaaagt gctcatagcg gcgacggttc atcagatagt cggcaatgat cacgccaccc
    20941 actggaggaa tagctgccga aaggaaggtc aaccagccga caaaattgtt atacagccat
    21001 aatgcacaga ccgtaccgat aataccgttg attaccgaaa gggttttgct cgacatcccg
    21061 gtaatgttgg cgaaacctaa acccgacgca tagagcgcgt tatcgttggt ggtccagata
    21121 tttagcccca gcaccacaat cgcaggcaac agcaggccct gagcaatcat cacatcagag
    21181 atatccgcca accccagtgc cgccgctccc gctgcaccga aaataaacat caacgagttg
    21241 ccgaggaaaa aggccaccat cgccaccagc accgccagtt tggcattgcg accaaagcgg
    21301 acaaagtcag cggtgagcgt acccgcgctg ataaatgacc ccacaaccag cgccagcgcg
    21361 acattgaaat ctaatggttg tgcgggaacg accgctttta aggcgtccag gccgcccatg
    21421 ccgttaacgg ccagccacac ggaataaccg ccaagacagg cgatcgccgg aaccgcaatc
    21481 accgaaagaa ctgtcagcgc cgaaatgcca aagaagacgg tgacggtcat cagtaaaccg
    21541 gaaacggcaa tcagcaaatt aatatccagc ccggttgcct tacccaccgg aatggcaaac
    21601 atcgccacgc caacgccaaa ccagccaacc tgggtgccgc ccagcagcag tgaaggcagc
    21661 catgagcctt taacaccaaa cgagaagcga gcaagaagat gagtggtcag gccggttttt
    21721 gcgccaatgt aaccgagaaa tgaagtgtaa ataccgagga gaagattgcc gatgagaact
    21781 gcgaggaaga aatcatgata gctaagaccg gttccgagag tgccgccagt ccacatactg
    21841 gcggaaaaga aggttaatcc cagcatgacg aacgtcaatg ccaatacccc tttccgcgcc
    21901 gactgcggga ctggcccctg gctaaagttg ttatcttgcg acacgaaatt cccccttttt
    21961 gctgtttgaa acccaaaaaa tccgccgcat tctattcatc tgaagataaa aagcaatcgt
    22021 tttcgtgggg aaatatattt tttatatgaa tgagtgcaaa aaggtggggg aaaaattatc
    22081 ggtgtaaaaa cagtgggtag gcctgataag acgcgtcagc gtcgcatcag gcaactgcgc
    22141 atggtgtcgg atgcggcgcg agcgccttat ccgacctacg gttcaggcct gtaggcatga
    22201 taagacgcgc ctgcgtcgca tcaggcatcg gcgcatggtg tcgaatgcgg cgcgagcgcc
    22261 ttatccgacc tacggttcag gcctgtaggc atgataagac gcgcctgcgt cgcatcaggc
    22321 atcggcacac ggtgtcggat gcggcgcgag cgccttatcc gacctacggt tcaggcctgt
    22381 aggcatgata agacgcgtca gcgtcgcatc aggcatctgc
//
LOCUS       DAZGBC010000078        22401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGBC010000078 DAZGBC010000000
VERSION     DAZGBC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.078"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(181..256)
                     /locus_tag="ACHK1J_002824"
     tRNA            complement(181..256)
                     /locus_tag="ACHK1J_002824"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Phe,seq:gaa)
     gene            complement(362..1069)
                     /gene="yqgA"
                     /locus_tag="ACHK1J_002825"
     CDS             complement(362..1069)
                     /gene="yqgA"
                     /locus_tag="ACHK1J_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO7912068.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHEPF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1222..1305
                     /locus_tag="ACHK1J_002826"
     CDS             1222..1305
                     /locus_tag="ACHK1J_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912069.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            1468..3603
                     /gene="speC"
                     /locus_tag="ACHK1J_002827"
     CDS             1468..3603
                     /gene="speC"
                     /locus_tag="ACHK1J_002827"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO7912070.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESQEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAVEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3653..4909)
                     /gene="nupG"
                     /locus_tag="ACHK1J_002828"
     CDS             complement(3653..4909)
                     /gene="nupG"
                     /locus_tag="ACHK1J_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO7912071.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5111..6190)
                     /gene="mltC"
                     /locus_tag="ACHK1J_002829"
     CDS             complement(5111..6190)
                     /gene="mltC"
                     /locus_tag="ACHK1J_002829"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO7912072.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6255..6530)
                     /gene="yggX"
                     /locus_tag="ACHK1J_002830"
     CDS             complement(6255..6530)
                     /gene="yggX"
                     /locus_tag="ACHK1J_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO7912073.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6558..7610)
                     /gene="mutY"
                     /locus_tag="ACHK1J_002831"
     CDS             complement(6558..7610)
                     /gene="mutY"
                     /locus_tag="ACHK1J_002831"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO7912074.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7771..8490
                     /gene="trmB"
                     /locus_tag="ACHK1J_002832"
     CDS             7771..8490
                     /gene="trmB"
                     /locus_tag="ACHK1J_002832"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO7912075.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8490..8816
                     /gene="yggL"
                     /locus_tag="ACHK1J_002833"
     CDS             8490..8816
                     /gene="yggL"
                     /locus_tag="ACHK1J_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO7912076.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            9000..9719
                     /gene="yggN"
                     /locus_tag="ACHK1J_002834"
     CDS             9000..9719
                     /gene="yggN"
                     /locus_tag="ACHK1J_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO7912077.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9895..10941
                     /gene="ansB"
                     /locus_tag="ACHK1J_002835"
     CDS             9895..10941
                     /gene="ansB"
                     /locus_tag="ACHK1J_002835"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO7912078.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11058..12065
                     /gene="yggM"
                     /locus_tag="ACHK1J_002836"
     CDS             11058..12065
                     /gene="yggM"
                     /locus_tag="ACHK1J_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO7912079.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINKACDRWKKDTEQLRRNGVI
                     K"
     gene            complement(12221..13357)
                     /gene="hemW"
                     /locus_tag="ACHK1J_002837"
     CDS             complement(12221..13357)
                     /gene="hemW"
                     /locus_tag="ACHK1J_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO7912080.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(13350..13943)
                     /gene="rdgB"
                     /locus_tag="ACHK1J_002838"
     CDS             complement(13350..13943)
                     /gene="rdgB"
                     /locus_tag="ACHK1J_002838"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO7912081.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREQKSAISHRGQALKLLLDALRNG"
     gene            complement(13951..14241)
                     /gene="yggU"
                     /locus_tag="ACHK1J_002839"
     CDS             complement(13951..14241)
                     /gene="yggU"
                     /locus_tag="ACHK1J_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO7912082.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(14238..14804)
                     /gene="yggT"
                     /locus_tag="ACHK1J_002840"
     CDS             complement(14238..14804)
                     /gene="yggT"
                     /locus_tag="ACHK1J_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO7912083.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(14822..15526)
                     /gene="yggS"
                     /locus_tag="ACHK1J_002841"
     CDS             complement(14822..15526)
                     /gene="yggS"
                     /locus_tag="ACHK1J_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO7912084.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            15544..16524
                     /gene="yggR"
                     /locus_tag="ACHK1J_002842"
     CDS             15544..16524
                     /gene="yggR"
                     /locus_tag="ACHK1J_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO7912085.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRQGISLALRLLPSHYPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(16708..17124)
                     /gene="ruvX"
                     /locus_tag="ACHK1J_002843"
     CDS             complement(16708..17124)
                     /gene="ruvX"
                     /locus_tag="ACHK1J_002843"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO7912086.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(17124..17687)
                     /gene="yqgE"
                     /locus_tag="ACHK1J_002844"
     CDS             complement(17124..17687)
                     /gene="yqgE"
                     /locus_tag="ACHK1J_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO7912087.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(17796..18746)
                     /gene="gshB"
                     /locus_tag="ACHK1J_002845"
     CDS             complement(17796..18746)
                     /gene="gshB"
                     /locus_tag="ACHK1J_002845"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO7912088.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(18759..19490)
                     /gene="rsmE"
                     /locus_tag="ACHK1J_002846"
     CDS             complement(18759..19490)
                     /gene="rsmE"
                     /locus_tag="ACHK1J_002846"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO7912089.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(19570..20277)
                     /gene="endA"
                     /locus_tag="ACHK1J_002847"
     CDS             complement(19570..20277)
                     /gene="endA"
                     /locus_tag="ACHK1J_002847"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO7912090.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(20372..20869)
                     /gene="yggI"
                     /locus_tag="ACHK1J_002848"
     CDS             complement(20372..20869)
                     /gene="yggI"
                     /locus_tag="ACHK1J_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO7912091.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(20946..22340)
                     /gene="galP"
                     /locus_tag="ACHK1J_002849"
     CDS             complement(20946..22340)
                     /gene="galP"
                     /locus_tag="ACHK1J_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO7912092.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5514 a         5696 c         5891 g         5300 t
ORIGIN      
        1 atgtaagcat acagtgttaa cgagaacagg gcgtctccgg aaaagacacc ctgtttgagg
       61 atacgatacc aaactcaatc agtgtaacca caacttacac aaacggagac cctttgatgc
      121 cgcatcctgt attaaccgcc cgtcaaggat ctcatccctt aaccagcgtg ttctggtata
      181 tggtgcccgg aggcggaatc gaaccaccga cacggggatt ttcaatcccc tgctctaccg
      241 actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc gtcaatcaaa
      301 tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat tgcacgcatt
      361 gtcaggcaaa ccaggcggtc caggctgcgg aaagcggcat tgccagaagg agtgctggaa
      421 gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt gccagcagca
      481 ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc ggtaatatca
      541 gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta attaccgaca
      601 ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga aatgacttgg
      661 cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag atcccggtgc
      721 cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaaggt tcatgcgctg
      781 gcttcttacg tgagtgacga aacagatttt gtgctttggc gaccgctgta ttgacgcctt
      841 tctccagcag acaaatttcg ccgattaatg ctccgagtaa ggttgctaaa accatcgctg
      901 gaaggttggc gcatttcacc accagtaaaa taccaatccc cagcgatgcc agaccaaaaa
      961 ttgatgtcat ggagacgcgg atacgttccg gtaagcgttg gctaagcagt gcaccgagca
     1021 tgccacccag taatattgca cttgcgttga taaaagggcc gatgaccaca agagttcctg
     1081 ttaactgtat tgtttgattt tcttattatg aactttttgc cttgttgatg gcttgtctgg
     1141 tcgcaaagtc ctggcttgca cgtcttagcg aaaggtgcca tgattgcgcg aattttctcc
     1201 tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc ttatcgccgt
     1261 ttcgcgcgaa acccttctct ttttatgcta ctgctcatgc ggtgaagtta acgcacgttc
     1321 actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg gcggtcggag
     1381 ctggtgacca gtttgaccca tatctcatgg ggcagggttt tacaccttgt ccggtattct
     1441 tacttccccg aaacgggttt tgcgcttatg aaatcaatga atattgccgc cagtagtgaa
     1501 ctggtatccc gactttctac tcatcgccgc gtggtggcgt tgggagatac tgattttacg
     1561 gacgtcgcgg cagtcgtcat taccgctgcg gatagtcgca gtggcattct tacgttgctt
     1621 aagcgcaccg gttttcatct accggtgttt ttgtattccg aacatgctgt tgaattacct
     1681 gcgggcgtta cggcggtaat caacggcaac gagcagcatt ggctggagct ggaatccgca
     1741 gcctgtcagt atgaagagaa tttgctgcca ccgttttatg acacgctgac gcagtacgtt
     1801 gagatgggca acagcacctt tgcttgccct gggcatcaac atggtgcgtt ttttaaaaag
     1861 catcctgccg gacgccattt ttacgatttc tttggtgaga acgtctttcg cgccgatatg
     1921 tgtaatgctg acgtaaaatt gggcgatctg cttattcatg aaggatcggc gaaagatgcg
     1981 cagaaattcg cagccaaagt ctttcatgcc gataaaacct attttgtact gaacggcaca
     2041 tcggcagcga ataaagtggt gacgaatgcg ctattaacgc gtggcgatct ggtgctcttc
     2101 gaccgtaaca accataagtc gaatcatcac ggcgcgctga ttcaggcggg ggcgacgccg
     2161 gtctatctgg aagcttcacg caacccgttt ggtttcattg gcggtattga tgcgcactgt
     2221 tttaatgaag agtatctgcg ccagcaaatt cgcgacgttg cgccagaaaa agccgacctg
     2281 ccgcgcccgt ttcgcctggc gattattcag ctgggaacct atgacggcac tgtctataac
     2341 gcccgtcagg tgatcgatac cattgggcat ctgtgtgatt acattctgtt tgattccgcg
     2401 tgggtcggtt acgaacagtt tatcccgatg atggcggata gctcgccgct gctgttagaa
     2461 cttaacgaaa acgatccagg gatctttgtg actcagtcgg tgcacaaaca gcaggcggga
     2521 ttctcacaga cgtcgcagat ccataaaaaa gataaccata tccgcggaca ggcgcgtttt
     2581 tgcccgcata agcggttgaa taacgccttt atgctccatg cttctaccag ccctttctat
     2641 ccgctgtttg ctgcactgga tgttaacgcc aaaattcatg aaggggagag tgggcgtcgg
     2701 ctgtgggctg agtgcgttga gttgggtatt gagtcgcgca aggctattct tgcgcgctgt
     2761 aagctgttcc gcccgtttat cccgcccgtt gttgatggca aattgtggca ggattatccg
     2821 acatcagtgt tagccagcga ccgccgtttt ttcagttttg agccgggggc gaagtggcac
     2881 ggctttgaag gatatgccgc ggatcagtat tttgttgatc cgtgcaagct gttactcact
     2941 acaccaggta tcgatgccga aaccggcgag tatagcgact ttggcgttcc ggcgacgatt
     3001 ctggcgcact atctgcgtga gaacggcatt gtgccggaga agtgcgatct caactccatt
     3061 ctgttcttat taactccggc ggaaagccaa gagaagctgg cacaactggt ggcgatgctg
     3121 gcgcaatttg aacagcatat tgaggatgac tcgccgctgg ctgaggtgtt gccgagcgtt
     3181 tataacaaat atccggtgcg ctatcgcgac tacaccctgc gccagttgtg tcaggagatg
     3241 cacgatctgt atgtcagttt cgacgtcaaa gacctacaaa aagcgatgtt ccgccagcag
     3301 agtttcccgt cagtggtgat gaacccccag gatgcgcata gcgcttatat tcgcggtgac
     3361 gtggagttgg tgcggattcg tgatgccgaa gggcgaattg cggtagaagg ggcgttgcct
     3421 tatccacctg gcgtgctttg cgtggtaccc ggggaagtct ggggtggggc ggttcaacgt
     3481 tatttccttg cactggaaga aggggtgaat ttgttgccgg gattttcgcc ggagctgcaa
     3541 ggtgtttata gcgaaaccga tgcggatggc atgaaacggt tgtacggtta tgtgttgaag
     3601 taagaataaa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt aattagtggc
     3661 taaccgtctg tgtacctgtc gggacacgaa cgtgtttata tttgaacatc gccatgaacg
     3721 cgaaggccag aaccacggag taaccagcga aaatcaacca tacggtctgc cagtcggtaa
     3781 tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg aggatacagc
     3841 cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt gccgggctaa
     3901 cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg caaccgtaaa
     3961 cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg taagcaaaca
     4021 gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta ataccgtagc
     4081 ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc gaaatcgaca
     4141 tgatgattga cgcatgctgc acaataaagc tgctggcaaa catcggatct ttgtcgaagc
     4201 tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc agcagcattg
     4261 agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca tcgaggccca
     4321 gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc ggcagagtca
     4381 gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc atgtggctta
     4441 attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg gtgccccaga
     4501 tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg cgatagtaag
     4561 agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc aatatcacaa
     4621 ggaacatcgc ctccggcgtc gtgacctgtg ccgccatgaa cagcgtgata gcgccaatgg
     4681 tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca atccccagca
     4741 gcgcaggcat aaagaccgcg gcgataccca gtgagctata aactgcgcca atagaagcac
     4801 cgtcaaactt cagggtaaca aacatatagg aaccgagggt cgtcagccaa cttccccaca
     4861 gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg ttaatttcct
     4921 cacatcgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag atgatgggat
     4981 tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca aacattccta
     5041 atttgcaaaa cgaaattgtg atatggataa catttttgcc cctgagcatc gtcaggggcg
     5101 gttagtggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta taaagataac
     5161 ggcgagattc cgcagaggga tggcgggtcg tcagcgtttg ataaacatcg cccggcgtca
     5221 tggtgttaat aatattagcc gcctgaatct tatcattcga aaagactcgc agcacgctgc
     5281 ctgcgccgcc gttataggcg gtgatgacgg cataacgccg ggatgtaggg ttatcaattc
     5341 cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca atattgctgg
     5401 caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag cggaacacat
     5461 ctttcccggc agtatgttgt accacctgca ttaaccccag cgcatcggaa cggctgaccg
     5521 catacgggtt aaaggaagat tcagtctgca taattgccag aatcagcgac tcatcaacgc
     5581 catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt ttatcaaggt
     5641 ggttcggcac catgttaatg gtgacgctgt agatgatacg cagcccgttg ctgcggctct
     5701 tcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc cagcgaatcg
     5761 gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa atcgtaatat
     5821 catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcaat aacgttttga
     5881 taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatggtttcg atagtaatcg
     5941 taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat ttcacgtagt
     6001 ccttaggacc agcgatcacc acctctttga agccccagat gttctcaata ttgtgggcaa
     6061 attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg ttataggtat
     6121 cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc agcgcgagat
     6181 attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagctgc cggaggcgtg
     6241 ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg cacctcttta
     6301 ccctcgaaca ggaagttgac catctcctgc tccagcagct tgcggtgctc ggcattcatc
     6361 atgttgagtt tcttttcatt aatcagcatg gtttgtttgt gctgccactg cgcccaggct
     6421 tctttggaga tctcgttata gatgcgtttt cccagttcgc cggggtacag ctgaaaatcc
     6481 tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat aaatcatcct
     6541 ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa caaacgctcc
     6601 acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag cgcattgcct
     6661 tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat atctaagtgg
     6721 aaatggctga aggtatgccg aaacgcggtc agctgcgtca ggttatcggc agcaatctgc
     6781 cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa acagtataaa
     6841 ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc gtgctgtaac
     6901 aggagaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc cggataaagc
     6961 gaccagctat tgttggtggc ggcaatacat ccgttttgta gcggacagag cgaacatttc
     7021 ggcttcgaac gcgtgcaaat catcgcgccc aaatccatca tcgcctgatt aaaccgttcc
     7081 acgccgaccg cgggcgtcac ctgctcgctt aaactccata atttattctc gacctctttt
     7141 ttcccaggcc agccgcttac agcatagcag cgcgccagca cccgtttgac gttaccgtcg
     7201 agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt ggaacgcccg
     7261 acgcccggca gtgctgcaac ttcctcaaag gtttccggga atttaccgcc gtgtaaggtc
     7321 gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc aagccccgtc
     7381 cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt cgggaagcgc
     7441 gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg caacatcact
     7501 tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagagt ttttcgcccg
     7561 tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat ggtcaccgaa
     7621 ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa ccggaacttt
     7681 ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa tgcccgtttt
     7741 cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc accggaattt
     7801 gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca ggggcgactg
     7861 accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt tgagttcagc
     7921 gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac gcttgagatt
     7981 ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccccga gcaggacttc
     8041 ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc gcatgaagag
     8101 ggcttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca taaaatgatt
     8161 cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca caaagcgcgc
     8221 cataataaac gccgtatcgt tcaggtgccg tttgccgaac tggtaaaaag caaactgcag
     8281 ctggggggcg tattccatat ggcgaccgac tgggaacctt atgcggaaca tatgcttgaa
     8341 gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta cgtaccgcgt
     8401 ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg tcacggagta
     8461 tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt cgtctgcgta
     8521 aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg cgattcccgg
     8581 aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac gaggttatcg
     8641 aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt ctgatctgca
     8701 tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag tggctggaag
     8761 agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg gactaagaaa
     8821 gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca tgcctacaaa
     8881 gatgcacgat cgagtaggcg ggataaggcg tttacgccgc atccggcatg gaaaacgcgt
     8941 actttgttat caatctgggg ccagcaaatg ctggcctgat ttgttcttga gggaagacta
     9001 tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc gctcacgccg
     9061 actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa accgtgcagg
     9121 tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg tataacggta
     9181 agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag gctgaactac
     9241 gtagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgagaaa gcccgtattg
     9301 ctctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc agccgtctga
     9361 ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg cgcagcgatg
     9421 gcctgacgtt ccactataaa gccattgatc aggttcgcgc tgaaggccag caattagtga
     9481 atcaggcaat gggcggaatt ttacaggaca gcattaatga aatgggcgcg aaagcggtgc
     9541 tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg ctgcaatcct
     9601 caatccaaac cgagtggaaa aagcaggaaa aagatttcca gcagtttggc aaagatgttt
     9661 gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat ttaaaataat
     9721 cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt tttgttacct
     9781 gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta acgtcaaatt
     9841 tcccatacaa agctaaggga taatgcgtag cgttcacgta actggaggaa tgaaatggag
     9901 tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc agcattggca
     9961 ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg tgactccgca
    10021 accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa tgcggtgccg
    10081 caactgaagg acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg ctcccaggac
    10141 atgaacgatg atgtctggct gacactggcg aaaaaaatta acaccgactg cgataaaacc
    10201 gacggcttcg tcattactca cggtaccgac acgatggaag aaaccgctta cttcctcgac
    10261 ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc gtccacctcc
    10321 atgagcgcag acggtccatt caacctgtat aacgcggtag tgactgcagc tgataaagcc
    10381 tccgctaatc gtggcgtgct ggtagtgatg aacgacaccg tgctggatgg tcgtgacgtc
    10441 accaaaacca acaccaccga cgtagcaacc tttaagtctg ttaactacgg tccgctggga
    10501 tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca caccagcgac
    10561 acgccgttcg atgtctctaa gctgaatgaa ctgccgaaag tcggcattgt ttataactac
    10621 gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga tggcatcgtc
    10681 agcgctggtg tgggtaacgg caacctgtat aaaaccgtat ttgacaccct tgcaaccgcc
    10741 gcgaaaaacg gcactgcagt cgtgcgttct tcccgcgtac cgacgggcgc taccactcag
    10801 gatgccgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct gaacccgcaa
    10861 aaagcgcgcg ttctgctgca actggctctg acgcaaacca aagatccgca gcagatccag
    10921 cagatcttca atcagtacta atcgcctcgc cccggtatcg tgccggggct ttttcacttc
    10981 agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa taaattattc
    11041 cgcgcgaggg ttttcgggtg aaaaagcaat ggattgttgg cacggcgctg cttatgttga
    11101 tgactggtaa tgtctgggca gatggcgaac cgccaactga aaatatctta aaagatcaat
    11161 tcaaaaagca gtatcacggc attctcaaac ttgatgccat caccttaaaa aatcttgatg
    11221 ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt gacgatctct
    11281 atacctgggt cggtcagttg gcagattacg aactgctgga acagacctgg acgaaagata
    11341 aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct ggctggtcgg
    11401 tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc gacgatatca
    11461 aaacgaataa taaatatctg atcgtgaata gcgaagattt caattatcgc tttagtcagc
    11521 ttgagtctgc gttgaataac cagaagaact cgattcctgc gctggaaaag gaagtgaaag
    11581 cgcttgataa gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg ggaaaagatg
    11641 caaacggtaa gcaaatgacc cgcgaagacg cctttaaaaa aattcaccaa cagcgtgatg
    11701 agtttaataa acagaacgat agcgaggcgt tcgcggttaa atatgacaaa gaggtttatc
    11761 aaccggcgat tgcggcatgt cataaacaga gtgaagagtg ttatgaagtg ccgattcagc
    11821 agaaacgaga tttcgatatc aacgaacaac ggcgacagac ttttctgcaa tcacaaaaac
    11881 ttagccgtaa attgcaggat gactggataa cgctcgaaaa agggcaatat ccgttaacga
    11941 tgaaagtttc agaaataaac agtaaaaaag tcgcgatttt gatgaagatt gacgatatca
    12001 ataaggcctg cgatcgctgg aaaaaagata ccgaacaact gcggcgcaat ggggtgatta
    12061 agtaagtttt gatatagcgc acgatttgta gcaggatgcg gcgttgaccc cgcatccggc
    12121 aatcaacgtc tgatgcgacg ctagcgcgtc ttatcagacc tacaaccgtt gccacatgta
    12181 ggctggataa ggcgttcacg ccgcatccgg caatacaagt ttactcagcc agaaaaagct
    12241 ccagcagcga atttaaaaac aacttcccat gttccgttat ctgccagtaa tccgcacatt
    12301 ctgtgagata accctgggca atcgcctcgt ctaactgtgg gcgaatcaca tcttcgcaaa
    12361 gcccggtata cgcactaaac tctgcgcgtg gcgcggcctc cagcaagcga aagcggttca
    12421 taaagaactc aaacggctta tctgcagctt cgacatcacg ctggctttcc agataccttc
    12481 cttgcataaa accacgcgga tgacgcgttt tggtggtacg cagaatgcgc ccatccggga
    12541 aggtcacttt gccgtgcgcg ccgcagccaa taccgatgta gtccccaaag cgccagtagt
    12601 tgagattgtg ctggcactgg taaccgggtt tggcgtaagc ggaagtttca tattgctgat
    12661 aacccgctgc ggttaataac tgatgcccct gttcgaatat atcccacaac gcgtcatcgt
    12721 ccggcagtac aggagggcgc gagccaaaca gcgtattggg ttcgatggtc agttgatacc
    12781 aggaaagatg cggcggattc agttcaatgg cctggcgcag atcgccaagc gcctcttcca
    12841 gtgattgatc cggcagccca tgcatcaaat caaggttaaa actacgcagc cccagcccgc
    12901 ttgccagatt cgcagctcgt ttcgcttctt gcgggccatg aatgcgccca agtcgtttca
    12961 gcttttcttc gctaaaactc tgtacgccaa tagagatgcg gttcacgcca gcacgctgat
    13021 aatcgacaaa gcgatcggct tctaccgtac cagggttcgc ttccatagta atttctgcat
    13081 ccgctgccag cggcaaacgc gcacgcacgc cgtccagcag cgtttgcatc gccgggccgg
    13141 aaagcaggct cggcgtacca ccgccaataa aaattgtctg cacttcacgg ccctgagcgt
    13201 aagccacatc gttgtccaga tcgttaagca gatgctgaac ataatcgtcg tgcggcactt
    13261 ctcctttcaa cgcgtgagag ttgaaatcgc agtacgggca tttctgcacg caccacggga
    13321 tgtgaatgta gagactcagc ggcggtaatt taaccattac gtaaagcgtc cagcagcagt
    13381 ttcaacgcct gaccacggtg ggaaatggcg ctcttttgtt cgcgagtcag ttcggcagcg
    13441 gttttccctt cggaaggtac gaagaagatt ggatcataac caaagccacc agtgcccgcc
    13501 ggttcacgag taatcactcc tggccagcta ccgtggcaca ccagcggagt cggatcttcc
    13561 gcgtgacgca gatacaccag cacgcagtgg aaacgcgcct gacgttggtc gtccggtacg
    13621 tctttcagtg tttccagcag tttttgcaga ttcttttgat cggtcgcgtc ttcaccggaa
    13681 taacgcgcgg agtaaatgcc tggcgcgcca ccaagtacat ctaccgccag accggagtcg
    13741 tcggcaattg caggtaaacc ggtcactttt gccgcatggc gcgctttcag aatcgcgttt
    13801 tcgataaagg tcaggccggt ttcttcagcg gaatctacgc cgaggtctgt ttgggccacg
    13861 atatcaagac cgaagtcgct aagcagcgac gccagctcac gcactttacc ggcattgccg
    13921 gttgcgagga caactttttg cataggatac ctaattaatt aacgccgcga tttctggcgg
    13981 gatttgttgc ggattaatga ttttaatttg tttgtggcgg ccaagttcgc ctttttcaat
    14041 caccacctgg cttttggcaa cccggaattg cttaccgaga aacttcacca gatgactgtt
    14101 ggcctggccg tcaaccggcg gcgcggttat ggcgactttt acctcgtcgc catgtaaacc
    14161 gacaatagaa tcacggctgg ctttcggctg aatataaagc cgtaaaacca gaccgtcatc
    14221 gttaactgtt acggcactca taacgccatc cacagccccg gcagcagcat gtttccggtt
    14281 gcctgtaata cttctgcgac acccatattg atgacataca gcagcagaac gaggatcatc
    14341 ggcgagaaat caatcccacc cattgccggt agcaggcggc gaatcgggcg cagcagcgga
    14401 tcggccagct gaatcagcac gtattcaatc gggctacggc cctggcttac ccagctcata
    14461 atcgccatca ccagcaggac ccagaaaatc agcaggccga tggttttcag cagaatcagt
    14521 aaaccggcaa tccagatgat tggcaggaag gtcaccactt taaacagcac gatggctttg
    14581 ataaaactga gaatataggc aaccagtagc gaggcgctat caatggggcc cattgccgga
    14641 ataacgcggc gcagtggccc gataattggc tgcgttacct tcactacaaa ctgtgagaag
    14701 gggttgtaaa aatcacaatg agcccactgc atccagatgc gtaataacag caccatggta
    14761 tacagctcaa tgaccgttga aagcaggaaa gtcaacgtat tcatggcgtt cctttaattc
    14821 cttatttttt agagtaatca cgcgcaccaa aaattgcagt gccgatacga accatcgtgc
    14881 tacctgccgc aatggcggct tccatatcgt ccgacattcc cagagagagc gtgtcgatat
    14941 gcgggtagcg cgttttcagt ccggcaaatg ctacagccat ttggcgtgca acttcaaact
    15001 gccttacata ttctgactca ggcgcaggga ttgccatcag cccgcgcaga cgtaaacgcg
    15061 gtagttcagc gaccgctgct gccagctcgt ccagttctgc cagttgaatc ccggacttac
    15121 tgttttcatc actaatatta atttgaatca gcacgttaag agggggaagt tctgccgggc
    15181 gctgatcgtt gaggcgggta gcgatgcgca aacggtcgat ggtatgacac cagtcgaaat
    15241 gctctgccac caggcggctt ttattagact gcaacgggcc aataaaatgc cattctaatc
    15301 ctgttacgcc cagttcctga aagtggcgaa ttttatctac cccttcctga acgtagtttt
    15361 caccaaattg acgctgcccg gcatcaatgg cttctgcgat ggcgctcgca ggttttgttt
    15421 tactgactgc aagcagcgta atttcttctg gagaacggcc gcaacgcgtt gcagccgctg
    15481 agattttgtc ccggacctgt gccaggttat gcgcaatatc gttcattttc cgaggatctt
    15541 agtatgaata tggaagaaat tgtggccctt agtgtaaagc ataacgtctc ggatctacac
    15601 ctgtgcagcg cctggcccgc acgatggcgc attcgcgggc taatggaagc tgcgccgttt
    15661 gatgcgccgg acgtcgaaga gctactgcgg gagtggctgg atgacgatca gcgggcaata
    15721 ttgctggaga atggtcagct ggattttgcc gtgtcgctga cggaaaacca gcgattgcgt
    15781 ggcagtgcgt tcgcgcaacg gcaaggtatt tctctggcgt tacggctgtt accttcgcac
    15841 tacccgcagc tcgaacagct tggcgcacca ccggtattgc cggaattact caagagcgag
    15901 aatggcctga ttctggtgac gggggcgacg gggagcggca aatctaccac gctggcggcg
    15961 atggttggct atctcaatca acatgccgat gcgcatattc tgacgctgga agatcctgtt
    16021 gaatatctct atgccagcca gcgatgtttg atccagcagc gggaaattgg tttgcactgt
    16081 atgacgttcg catcgggatt gcgggccgca ttgcgggaag atcctgatgt gattttgctc
    16141 ggagagctgc gtgacagcga gacaatccgt ctggcgctga cggcggcaga aaccgggcat
    16201 ctggtgctgg caaccttaca tacacgtggt gccgcgcagg cagttgagcg acttgtggat
    16261 tcatttccgg cgcaggaaaa agaccccgtg cgtaatcaac tggcaggtag tttacgggca
    16321 gtgctgtcac aaaagctgga agtggataaa caggaaggac gcgtggcgct atttgaattg
    16381 ctgattaaca cacccgcggt ggggaatttg attcgcgaag ggaaaaccca ccagttaccg
    16441 catgttattc aaaccgggca gcaggtgggg atgataacgt ttcagcagag ttatcagcag
    16501 cgggtggggg aagggcgttt gtgacgttgc catgcccgat gtgcaagctt atcgggccta
    16561 caaaggcaat aaacgtctga tgcgacgctt gcgcgtctta tcagacctac aaccgctgcc
    16621 acatgtaggc tggataaggc gttcatgtcg catccggcaa taagacgcga tgatattatt
    16681 tacactccgc caggctttta aatcgcctta atatccctgc tcgaaatagc tttcgagaat
    16741 aataaccgca gaggcagagt cgactttgcc tttgttaagc gcccgatagc cgccctgttc
    16801 aaacagaccg gaacgggctt ccacggtgct aagacgctcg tcatggagct ttacttcaac
    16861 accgaaacga ccatgaatac ggttggcaaa tttacgcgct ctggcggtta acggctgttc
    16921 ggtaccgtcc atattcagcg gcaaaccaac gatgatttcg tccggctgcc actctttcag
    16981 taaacgttcg atgagattcc agtccggcgt accgtcctgt gctttaattg caggcaatgg
    17041 gcgagcggtg ccggtaatgc gttggccgac cgctacgcca atgcttttgg taccgaagtc
    17101 gaaggcgagt aaggttccac tcatcaggcg tgtcctgcca caccaggcat ggtgagaata
    17161 tccacaccaa tcagttttgc cgcctcgcgc cagcggtcgg caattggcgt tttgaacaga
    17221 atattcagat ctgccggggc ggttagccac gcgttatcga gaatttcttg ttccagttga
    17281 cctttctccc aggaggcata acccagagcc accaatacgt cagacggttg tttatcggtg
    17341 ccgagcgttt ccagcacatc gcgggaagtg gtcattaccg tgttgtctga aatgcgaatg
    17401 ctggaagcaa aattagaggg cggagtatgc aaaataaacc cgcgatcttc agccagtggg
    17461 ccgccgagca taaccggttt atccagacgg attgattcat cacgcggctc cggcgtgatc
    17521 ttcagctttt ccagaatccc ttcaattttg agattttcca gcggcttgtt gacgatgatc
    17581 cccattgcac cattggtatt atgttcgcaa atgtagacca cggaacgacg gaaaatcgga
    17641 tcctggagag caggcatggc aataagaaag tgatgctgta aattcattgt cagaggttct
    17701 gtttcctggt tcaaaaaagc aacagcgccc agtatgcgtg gaaagaaggg cgctgtcact
    17761 cagagtctca acgagatcct tctcgctaag gtgggttact gctgctgtaa acgtgcttca
    17821 atggcatcca ttaacattcc agtgatcgac accggaaact ctgcttcaat ctcacgaata
    17881 caggttgggc tggtgacgtt aatttcagtc agacggtcgc cgatgatatc cagaccaaca
    17941 aaaatcagcc ctttttcttt cagcgtcggc ccgatctgac gggcgatttt ccagtcactt
    18001 tccgtcagcg gacgaggttc accgcgacca ccggcagcca gattgccacg ggtttcgccc
    18061 ccctgcggaa tacgcgccag gcagtacggt accggctcgc catccaccac cagcacgcgt
    18121 ttgtcgccat ctttaatggc tggcaggtaa ttttgcgcca tgcagtagcg agtgccatgc
    18181 tcagtcaggg tttcggcaat cacgccgagg tttggatcgc cttctttcac gcggaaaatc
    18241 gacgcgccgc ccataccgtc cagcggctta agaatgatgt cgctgtgttt ctcccagaac
    18301 gcttttagct gcgctttatt gcgcgtaacc agcgtttctg gcgttaagtc agagaaccag
    18361 gcggtaaaca gtttctcgtt acagtcgcgc aggctctgcg gcttgttaac gatcagcgtc
    18421 cctttctctt cggcacgttc cagaatatag gtcgcgtaga taaactcggt atcaaacggc
    18481 gggtctttac gcatcaggat cacatcgaga tcggccagcg gcagatcctg ttcaccgacg
    18541 aatgaaaacc actcttcgta gttctgcttc acgttcagcg tgcgggtatg ggcgcgggct
    18601 tcgccattga tcagatacag atcgcccatc tccatatagt gaagttcgta accacgacgc
    18661 tgtgcttcca gcaacatagc gaaactggaa tctttcttga tgttgatgtt tgcgatgggg
    18721 tccatcacga tgccgagctt gatcattatt cttctccgtt agcccaaatc gccaaatcgt
    18781 acttgtagcg cggtaatggc ggtgagcgca gttgtctctg tacgcaaaac gcgaggtccc
    18841 aacaggatat cagtaaattg atagcgggca gtcatggcaa tttcatctgc cgataaaccg
    18901 ccttccgggc caatcagcag gcggacgcgt tcaaccggta aaggcaacgt attgatgctg
    18961 ttactggcgc gcgggtgaag attcagtttc agtccttcat cctgctctgc acaccaggct
    19021 tccagatcca tcgctggacg gatttccggc actcggttac gaccacactg ctcacaggca
    19081 gcaattgcaa tcttctgcca ctgctggagc ttcttgttca gacgttcact atccagttta
    19141 acgccgcagc gctcagaaaa aagtggcgta atgaggctta caccgagttc gatcgatttc
    19201 tggatcgtaa attccatttt ttcaccacgc gacatcacct gaccgaggtg aatatgcagc
    19261 ggagattcgc gatcgtcgat ctggccttcc agcaccttca cttccacgct ttttttgctg
    19321 gcgctggtaa tttcggcgtc aaagacctgg ttgctgccgt caaacaattg caacgcctgc
    19381 cccggcccca tgcgcagtac gcgcccgata tggttggcgg catcttcgca aagcgcgatg
    19441 tgagaatggc tggtcagtgg ttcaggatga taaatgcggg ggatacgcat agttaaaaat
    19501 ccgcgtcgtc tccccacgcg gttgtacgcg tggggtaggg gttaacaaaa agaatcccgc
    19561 tagtgtaggt tagctctttc gcgcctggca agcgcgttgc acatacgggt tatgattgcc
    19621 ctgcaccttc gcgatgcgtt catcgcgctc gcactcccag tcggtaaccg gatacatctt
    19681 gttccatgcg ttgaacagct gcgtttgctg gcgagagagt gtcaggttgt attggtcacg
    19741 catatagaag taggtgcgcg caatggcacc gcgtgcacgc gctggtggtt cggcagcttt
    19801 ttctttgaaa tcgaccttca tggcgcattg accgtactgg ccttcgccgc cattccactg
    19861 gctgtacata aagttgccgc gatcgccatt cacctcgccg actgacggct gcaggttatg
    19921 catatcgctt tccatcttgc gatagaccgg atctttagcg cagtttttac gtccaccgtc
    19981 ctgccagcac tggcgctggt gaccgaattg ccaggcggga acgacgtgtt cccactctac
    20041 gcggctggcg cggttttcat ttttgcgcac ctgatagccg cacgattgca gatcaacaac
    20101 gccttttttg ccctgccagt taattttaca tccgcaataa aacgtaccgg gcgcgtcagc
    20161 gtggactttt accgccgcgg ctttcgcctg agaaaaacta ttgatacctt cggccaacgc
    20221 cgggccggaa aatgctgcgc tcagtaccac cgcagcaata gacaaataac ggtacatcac
    20281 ttactccgtg ttaaaacgag ccagcaacgt agcgaaagtt gttttggtat gcaatcagcc
    20341 agatcaggaa agttcctgat agttcagatg gttattttgc gaccagttgt tcgccgcagt
    20401 gaacacagcg atagacggct tcgccacgaa ctacgcgatt atggcggcgt acggtaagct
    20461 gatgctcctg gcacttgcag cggtagggga aggtgttgcg acgcacggat tgcagttcga
    20521 actgatgcgt acgacgggct ggaacgccca gcacactttc catcatccac ttccactctt
    20581 tgccatgtgg tgcaacgcgg ccaaaatgtt tccataccag caaatgtgcc agttcgtgcg
    20641 gcaccacttc ttcaataaag gcttcactgt tttccaacag caaaacggga ttgaggcgga
    20701 tttcatagct ttccagccag gctgttccgg cggaggttcc gcgctgggtg taagagagtt
    20761 ttggctccgg gtagttacgc cctagcttca ggttggcctg ggcgagtttt tcccgcaggc
    20821 gacgcataac ggcctgttgg atggcgatag ggagacggga tgttttcata gcggcagagg
    20881 atagagcgaa gaaaagatga ctgcaagagg cggcttcctc cgcgacggga ggaagcctga
    20941 ggagattaat cgtgagcgcc gatttcgcgc agtttacgac ctttcatcag attacgttca
    21001 atatgttcca gcgaaacgtg tttggtttcc ggtaccagcc acaatgtcag caggataaac
    21061 agtacgttca gagccgcata cacccagaag gtgttggcgt tacccagcgt gttgagcatg
    21121 gtcaggaacg ttgcgccaac gatcatgttg gcaatccagt tggtggcggt ggagcaggtg
    21181 atgccaaaat cgcggccttt cagcggctga atttcggagc acagtaccca aatcagcgga
    21241 ccggcactca tggcaaaacc gacaataaac atcagcagca tggcgatggc gaaatactgc
    21301 gccgacggag agtggatacc gatatgcatc attgtaccga gtacgcccat gccagcagcc
    21361 atcaccagga agcccagcgt tagcgttggt ttacgtcccc agcggtcaac aagaccgatt
    21421 gcgataaagg tggcaagtac gttggtcagg ccgacaatca ctgtccccca catttgctcg
    21481 gtagtgttgg tataacccgc cagttcgaag attttcggcg cgtaatacat gatgacgttc
    21541 atcccggtga attgctgcat tacctgtaac agtacgccaa ggaacaccgc gcggcggaag
    21601 ttgctattct ctttaaacag cgcccagcca ctctgtttaa cctgcaaact ttcacggatt
    21661 tcatccagtt cgcgtttcgc ttccgcgctg gtgtcacgca ggcgtagcag cacgcgttcg
    21721 gcatcaacaa aacggcgttt ggcggcaaac caacgtgggc tgtctggcag gaagaagaca
    21781 ccaatcagca gcaaaattgc cgggatgata atcacaccca gcatccagcg ccatgcaccg
    21841 gtgtagctga aggcagtatc agaaagataa gcaccgagga tcccgatagt gatcatcaac
    21901 tgatacatcg agatcatact gccgcgaatt ttttccggcg caatttcaga gaggtacagt
    21961 ggtgcggtat aagaggccac acctaccgcc agccccagca gaacgcggga aagaatcagt
    22021 acttcaacgt ttggcgcagc cgcagagaac agcgaaccgg caacaaacaa aattgcgccg
    22081 atcatcaggc tctttttgcg cccgagttta aaggagagcc agccgctgcc caccgcaccg
    22141 actgccgcac cgaacatcat ggagcttacg acccattctt gcgtgtgcga agtaatctgg
    22201 aattcatctg caataaacgg cagtgcgcca gcaattacac cgatatccag gccaaagagt
    22261 aatcccgcca gagcggcaag gaagcagacg aaaaacgtca ttgccttgtt tgaccgcccc
    22321 tgttttttag cgtcaggcat gatgccctcc aatatggtta ttttttattg tgaattaaga
    22381 taggtgagta cgacgtaaaa a
//
LOCUS       DAZGBC010000079        22168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGBC010000079 DAZGBC010000000
VERSION     DAZGBC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.079"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHK1J_002850"
     CDS             complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHK1J_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO7912093.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTISG
                     SLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            1263..1799
                     /gene="sutR"
                     /locus_tag="ACHK1J_002851"
     CDS             1263..1799
                     /gene="sutR"
                     /locus_tag="ACHK1J_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO7912094.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHK1J_002852"
     CDS             1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHK1J_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO7912095.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTDPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHK1J_002853"
     CDS             complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHK1J_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO7912096.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4377..4553
                     /gene="hicA"
                     /locus_tag="ACHK1J_002854"
     CDS             4377..4553
                     /gene="hicA"
                     /locus_tag="ACHK1J_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
                     /protein_id="HFO7912097.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKVRFHGMHSVMPRHPCDEIK
                     EPLRKAIQKQLGLS"
     gene            4599..5015
                     /gene="hicB"
                     /locus_tag="ACHK1J_002855"
     CDS             4599..5015
                     /gene="hicB"
                     /locus_tag="ACHK1J_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO7912098.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHK1J_002856"
     CDS             5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHK1J_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7912099.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQVVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHK1J_002857"
     CDS             6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHK1J_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO7912100.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVKQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHK1J_002858"
     CDS             7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHK1J_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7912101.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVLVSWSRDVMVPLV
                     EER"
     gene            8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHK1J_002859"
     CDS             8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHK1J_002859"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7912102.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPIAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHK1J_002860"
     CDS             9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHK1J_002860"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7912103.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10669..12093
                     /gene="patD"
                     /locus_tag="ACHK1J_002861"
     CDS             10669..12093
                     /gene="patD"
                     /locus_tag="ACHK1J_002861"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO7912104.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12190..12285)
                     /gene="yncL"
                     /locus_tag="ACHK1J_002862"
     CDS             complement(12190..12285)
                     /gene="yncL"
                     /locus_tag="ACHK1J_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO7912105.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12480..12653
                     /gene="ortT"
                     /locus_tag="ACHK1J_002863"
     CDS             12480..12653
                     /gene="ortT"
                     /locus_tag="ACHK1J_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO7912106.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12739..12972
                     /gene="ydcY"
                     /locus_tag="ACHK1J_002864"
     CDS             12739..12972
                     /gene="ydcY"
                     /locus_tag="ACHK1J_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO7912107.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(12973..13422)
                     /gene="ydcZ"
                     /locus_tag="ACHK1J_002865"
     CDS             complement(12973..13422)
                     /gene="ydcZ"
                     /locus_tag="ACHK1J_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7912108.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13419..13937)
                     /gene="mddA"
                     /locus_tag="ACHK1J_002866"
     CDS             complement(13419..13937)
                     /gene="mddA"
                     /locus_tag="ACHK1J_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO7912109.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14118..15155
                     /gene="curA"
                     /locus_tag="ACHK1J_002867"
     CDS             14118..15155
                     /gene="curA"
                     /locus_tag="ACHK1J_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO7912110.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVEESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKIFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15353..16018
                     /gene="mcbR"
                     /locus_tag="ACHK1J_002868"
     CDS             15353..16018
                     /gene="mcbR"
                     /locus_tag="ACHK1J_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO7912111.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16054..18156)
                     /gene="pqqU"
                     /locus_tag="ACHK1J_002869"
     CDS             complement(16054..18156)
                     /gene="pqqU"
                     /locus_tag="ACHK1J_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO7912112.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            18398..19459
                     /gene="yncE"
                     /locus_tag="ACHK1J_002870"
     CDS             18398..19459
                     /gene="yncE"
                     /locus_tag="ACHK1J_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO7912113.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISVI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(19574..21073)
                     /gene="ansP"
                     /locus_tag="ACHK1J_002871"
     CDS             complement(19574..21073)
                     /gene="ansP"
                     /locus_tag="ACHK1J_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO7912114.1"
                     /translation="MSKHNTDTSEQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21341..21958
                     /gene="yncG"
                     /locus_tag="ACHK1J_002872"
     CDS             21341..21958
                     /gene="yncG"
                     /locus_tag="ACHK1J_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO7912115.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
     gene            22034..>22168
                     /locus_tag="ACHK1J_002873"
     CDS             22034..>22168
                     /locus_tag="ACHK1J_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFO7912116.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVKMFHLLSQAFYNLK
                     M"
BASE COUNT         5634 a         5461 c         5691 g         5382 t
ORIGIN      
        1 atcggtttct gtcagcgatt aaattcaaca ccacgtaaca aacgcctccg gcaattaatc
       61 cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc agaaatgcca
      121 ccaccgccgc gtctcgctca cgctcattat gcagcgcctg atacaaactg ccgctaatgg
      181 tacttaacag cgccagacct gccagcatct ggatccaact tacgggcagg gcagccatca
      241 tcccggtaat agcactacca aacagacctg cgatcaaata aaaaatgcct gcaacggcag
      301 ctgccagcca acgttgatct ttatccggat gcgcttccgg gctttggcaa atagccgcgg
      361 tgattgccgc aataccgacg gaataaacgc cgaaagggga aaaaaccagt gccagcaatc
      421 cagtaaatac aattaatggc gaaacaggag ccgaataccc ggctgctttc attgctgcga
      481 tacccggtgc gttttgcgat gccatcgtca ccagaaaaag ggggagtgca acgctcaggc
      541 tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag aacgggttta aagacaacat
      601 cagttgtgac aacgtcacct tgcgcgataa cgatcacgat cccaataatc atcgcggcaa
      661 ttaccgcata gcgcggcgca acggccttgg ttgccagcca taccagcaac atacttccac
      721 acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa accaaagcgt aataaaatcc
      781 cggcaagcat tgccgccgca agcgagtgcg gaataatgcg catcagacga gcaaagagtc
      841 ccgttatgcc gcagaggact attaacgcgt tggtgacaat aaaaacgccg atggcttcgt
      901 taagtgttag tccctgcaat ccggtgacca acaaagccgc gccaggcgtt gaccatgcgg
      961 tgagaacagg tacgcgatac cataatgtca gagtcagcgt actgacgccc attgccagcc
     1021 ccagcgccgt catccagcca gagatttgtg cagtggtggc tccggcgaca atcgctgctt
     1081 gccagattat tgccgctgaa ctggcgtagc caattaatac cgccagaaac cccgccagta
     1141 gcgtgggtgg aggaatagaa aacagacgca tagtcacctc gtgcgttata gcgtacaggc
     1201 accgtagcat ttgtccgtta taacgcacaa gtgataaact tccgttttgc cggaggagtc
     1261 gcatggaaaa tctcgctcgc tttttatcca ccacacttaa acaactacgt cagcagcgcg
     1321 gctggagtct ttcgcgactg gcagaagcga cgggcgtttc taaagcaatg ttaggacaga
     1381 ttgagcgtaa tgagtccagc ccgacggtcg cgacattatg gaaaattgcc actgggttga
     1441 acgtgccatt ttcgacattt atttctccgc cacagtccgc tacgccttca gtttatgatc
     1501 cacaacagca ggcgatggtg atcatttcgc tgtttcctta tgatccacaa ctctgctttg
     1561 aacacttctc gattcagatg gcacccggtg caataagcga atcgacgccc catgagaagg
     1621 gggtaatcga acatgtcgtc gtcattgatg gacaacttga tctgtgcgtt gatggtgaat
     1681 ggcagaccct taattgtggg gaaggcgttc gatttgctgc agacgtcacg catatctatc
     1741 gtaatggtgg ggagcaaacc gtacattttc attccctcat ccattacccg cgcagttaag
     1801 cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat agccgccatt tttcagctac
     1861 tggataagaa tgtgaccgta tcttctcatc gacttgaact gttaagcccg gcacgcgatg
     1921 ccgccattgc ccgcgaagct attttgcacg gtgccgatgc tgtttatatc ggcggccctg
     1981 gttttggtgc ccgtcataat gccagtaata gcctgaaaga tattgccgag ctggtgccgt
     2041 ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa caccattttg catgatgatg
     2101 agctggaacc cgcgcaacgg ctgattactg acctctacca gaccggtgtc gatgcgctga
     2161 ttgttcagga tatggggatt ctggaacttg atattccgcc gattgaactg cacgccagta
     2221 cgcagtgcga cattcgtaca gtagaaaaag cgaagttcct ttctgatgtt ggcttcacgc
     2281 agattgtgct ggcgcgagag ctgaatcttg atcagatccg cgcgattcac caggccacgg
     2341 acgcgaccat tgaattcttt attcatggcg cactgtgcgt ggcctattcg ggtcagtgct
     2401 acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg cgattgctcg caggcgtgcc
     2461 gtttgccata cacattgaaa gacgatcagg ggcgggtggt ttcctatgaa aaacatctgc
     2521 tgtcgatgaa agataacgat cagactgcca acctcggcgc gctgattgat gctggcgtac
     2581 gctccttcaa gattgaaggg cgttacaaag atatgagcta cgtgaagaat atcaccgccc
     2641 attatcgcca gatgctggat gccattattg aagaacgtgg cgatctgacg cgcgcttcat
     2701 caggtcgtac tgaacatttc tttgttccat cgacggaaaa gactttccac cgtggtagca
     2761 cagattattt tgtgaatgcc cgtaaaggcg atattggcgc gttcgattcg ccgaaattta
     2821 tcggcctgcc ggtgggtgaa gtattgaaag tggcgaaaga tcatctcgat gttgccgtta
     2881 ccgatccact ggcaaatggc gatggcctga acgtgttgat taaacgtgaa gtcgtcggtt
     2941 ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta ccgcgtctgg cccaatgaaa
     3001 tgccagcaga tttgcacaaa attcgcccgc atcacccact aaaccgtaat cttgatcata
     3061 actggcagca ggcactgaca aaaacctcca gcgaacgtcg ggtggcggta gacattgaac
     3121 tgggcggctg gcaggaacaa ctgattctga ccctcaccag tgaagagggg gtcagcatca
     3181 cgcatacgct ggatgggcag ttcgacgaag ccaataacgc cgaaaaagca atgaacaatc
     3241 tgaaggatgg tctggcaaaa ctggggcaaa ccatctatta cgcccgcgat gtgcaaatta
     3301 atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa ccagttccgc cgtgaagctg
     3361 ctgacatgct ggatgctgcg cgtcttgcca gttaccagcg cggcagccgt aaaccggttg
     3421 ctgatcctgc gccggtttat cctcaaacac atctgagttt cctcgcgaac gtttacaacc
     3481 agaaagcgcg tgaattttat catcgctatg gtgtgcagct gattgacgcg gcgtatgagg
     3541 cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa gcattgtctg cgctttgcct
     3601 ttaatctgtg cccgaaacag gcgaaaggca atatcaaaag ctggaaggcg acgccaatgc
     3661 aactggtaaa cggcgatgaa gtattaacgc taaagtttga ttgccgccca tgcgagatgc
     3721 acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc gttaccggga agcgtagtgg
     3781 catccgtaag tccggatgag ctgctgaaaa cattgccgaa gcgaaaaggg taaaacgcca
     3841 gttttctggt tactcacaac ttattgaatc tgcatgatat tgcctgccgg gtaaggcgtt
     3901 acgccgcatc cggcatcaaa cgactcaacg ttgtcccgct tctggtttgc gcgatttttg
     3961 ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc gccacagcac gcaattcatc
     4021 gctgtccgct tcatgacgca gctgatgatc cacattcttt acccacacaa attcatgtcc
     4081 tttgtgccct gccatgagtt gtcctgagaa cagcgcacac gttaataaga caaccgataa
     4141 cgccttcgta aacatcctgc cacctttttc ttaccttttg ccgctatgat gccgatcgtt
     4201 tcttgaggtt attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc
     4261 tgattttgca ttgcattctg ccgttgcggc aatttagtgc tattttcgag caaattacac
     4321 acggaggtaa acactaatgt ttactttgtt gatatactca gcggcaggga ggcgatgtga
     4381 aacaaagcga gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggcagca
     4441 accatttgaa agtcaggttt catgggatgc acagtgtcat gccgcgtcac ccctgcgatg
     4501 agattaaaga accattgcgt aaagcaatcc agaaacaact cggtttgagt taatcgccaa
     4561 ttaaaaaggt taatgacatg cgagagacag tcgaaattat gcgttatccc gtcactctta
     4621 cacccgcgcc ggaaggcggt tatatggttt cttttgtgga tatccctgaa gcgttgaccc
     4681 agggcgaaac tgtcgctgaa gcgatggaag cggcaaaaga tgctttactg accgcatttg
     4741 atttttattt tgaagataac gagcttatcc cgttaccttc gccattaaat agtcacgatc
     4801 attttattga agtacctttg agcgtcgcct ctaaggtatt gctgttaaat gcttttttac
     4861 agtcagaaat cactcagcaa gagttagcca ggcgaattgg caaacctaaa caggagatta
     4921 ctcgcctatt taacttgcat catgcgacaa aaatcgacgc cgtccagctc gcggcaaagg
     4981 cgcttggcaa agagttatcg ctggtgatgg tttaattaca gttaacgaaa agttgtcatt
     5041 tttaacaact gatatagact gccgaatcat ctgcacataa ttacgattcg ataatgaaaa
     5101 aataccagca gcttgcagaa caattacgcg agcagattgc gtcgggtatc tggcaacccg
     5161 gcgatcgttt accttcgttg cgtgaccagg tggcgctttc tggcatgagc tttatgactg
     5221 tcagccatgc ctatcagttg ctcgaaagtc agggatatat tatcgcacga ccgcagtcgg
     5281 gttattacgt tgcgccacag gcaataaaaa tgccgaaagc gccagtcatt ccagtcactc
     5341 gagatgaagc agtcgatatc aacacttata tttttgatat gttgcaggcc agtcgcgatc
     5401 cgtcggtcgt tccgtttgcc tcggcctttc ccgacccgcg acttttcccc ctccaacaac
     5461 taaaccgctc gctggcgcag gtaagcaaaa ccgccacggc gatgagtgtg attgaaaact
     5521 taccgccagg aaacgcagaa ctgcgtcagg ctattgctcg tcgctatgcc ttacagggca
     5581 tcaccatttc tcctgatgaa attgtcatta ctgccggggc gttagaggca ttaaacctca
     5641 gtttgcaagt ggtaactgaa ccgggcgatt gggtgatagt agagaatcct tgtttctacg
     5701 gtgcgttgca ggcgctggag cggctacggc tgaaggcgtt atcggtggcg acggatgtta
     5761 aagaagggat cgatcttcag gcgctggaac tggcgttgca ggagtatccg gtgaaagcgt
     5821 gctggctgat gactaatagc cagaatcctc tcggatttac cttaacgccg caaaaaaaag
     5881 cacaactggt ggcgttgctc aatcagtaca acgtaacgct gattgaagat gacgtttaca
     5941 gcgaacttta ttttggacgg gaaaaaccgc tgcctgcgaa agcgtgggat cgccacgatg
     6001 gcgttttgca ttgctcttcg ttttcgaaat gtctggtgcc tggttttcgt attggttggg
     6061 tcgccgccgg aaaacatgca cgtaaaattc aacgcttgca gttgatgagt acgctttcca
     6121 ccagctcacc gatgcagctt gcgctggtgg attacctttc cacgcgccga tacgacgccc
     6181 atcttcgtcg cctgcgtcgc cagcttgcgg aacgtaaaca acgtgcctgg caggcactgc
     6241 tgcgttacct gcctgcggaa gtgaaaattc atcataatga cagcggttat tttctctggc
     6301 tggagctccc cgagccgtta gatgccggcg aattgagcct ggcggcactg acgcatcata
     6361 tcagtattgc gccgggtaaa atgttttcta ccggtgaaaa ctggtcacgt ttttttcgtt
     6421 ttaataccgc gtggcagtgg ggagaacgtg aagaacaggc ggtaaaacaa ttaggcaaac
     6481 ttattcaaga acggctgtaa tagcgtttaa tttaattcct cttagattgg gtaatatgaa
     6541 tttcgaatag cagtcatatt tcctaactcc ttgtctatac tccagaagat aaccttacag
     6601 acggcataat gcgcggtagc tcacaacctg attaaatttt ctcaggggcg aaggtgtgcc
     6661 tgcaagccgc cgtctatggt taaacaagga gatattttta cggcacggcg gctgaacaat
     6721 taattacgac aggagtaaga ccttatgagc aagacatttg cccgcagcag cctgtgtgcg
     6781 ctcagcatga caataatgac cgctcacgcc gccgaacccc ctaccaattt agataaaccg
     6841 gaagggcgac tggatattat cgcctggccg ggatatatcg aacgcggaca aactgataaa
     6901 caatacgact gggtaaagca attcgaaaaa gagacaggct gcgcggtgaa tgtgaaaact
     6961 gcggcgactt ccgatgaaat ggtcagcctg atgaccaaag gaggttacga tctggtcacg
     7021 gcgtccggcg atgcctcgct gcgtttgatt atgggtaaac gcgtgcagcc gattaatacc
     7081 gccttgattc ccaactggaa aacgctcgat ccgcgcgtgg ttaaaggcga ctggtttaac
     7141 gttggcggca aagtttacgg cacaccttac caatgggggc cgaacctgct gatgtacaac
     7201 actaaaacct tcccgacgcc gccggatagc tggcaagtgg tttttgttga gcaaaatctg
     7261 ccggacggca agagcaataa aggccgcgtt caggcttatg atggccctat ctatattgcg
     7321 gacgctgcgt tgttcgttaa agccactcag ccgcagttgg gcatcagcga tccgtatcaa
     7381 ctcaccgaag aacagtacca ggcggtgctg aaagtgctgc gcgctcaaca cagtttgatc
     7441 catcgctact ggcatgacac taccgtgcaa atgagcgatt tcaaaaacga gggtgtggtc
     7501 gcttccagtg cctggcccta tcaggccaac gccctgaaag ccgaaggcca gcctgttgct
     7561 accgttttcc cgaaggaggg tgttaccggt tgggctgata ccaccatgct gcatagcgaa
     7621 gcgaaacatc cggtttgcgc ctacaaatgg atgaactggt cattaacgcc aaaagtgcag
     7681 ggcgatgtgg cggcctggtt tggctcgtta ccggtagtgc cggaagggtg taaagccagt
     7741 ccgttattag gcgaaaaagg ttgtgaaacc aacggtttta actatttcga caaaatcgcc
     7801 ttctggaaaa cgcctatagc agaagggggc aagtttgttc cctacagtcg ctggacgcag
     7861 gattacattg ccattatggg cggtcgctaa cttcgctggg gtgctttatg acgtacgcag
     7921 tggagtttga caacgtctcg cggttgtacg gtgacgtgcg cgcagtagat ggcgtcagta
     7981 ttgcgataaa agatggtgag ttcttctcta tgctggggcc gtccggctcc ggcaaaacca
     8041 cctgcctgcg cctgattgct ggcttcgaac agctttccgg cggggctatc tctatctttg
     8101 gtaaacccgc cagcaatctg ccaccgtggg agcgggacgt gaatactgtc tttcaggact
     8161 acgcgctatt tccgcatatg tcgattcttg acaatgtcgc ctatgggctg atggtcaaag
     8221 gcgtgaataa aaagcagcgg cacgcaatgg cgcaagaggc gctggagaaa gtggcgttgg
     8281 ggtttgtaca gcaacgtaaa ccgtcacaac tttctggtgg tcagcgccag cgggttgcta
     8341 tcgccagagc attggtgaat gaaccgcgcg tattgctgtt ggatgaaccg ctcggcgcac
     8401 tggatctcaa attgcgtgag cagatgcagc tggaactgaa aaaactgcaa cagtctctcg
     8461 gtatcacttt tatcttcgtt acccacgatc agggcgaagc gttatcgatg tccgatcgtg
     8521 tggcggtttt caataatgga cgcattgagc aggtcgattc cccgcgcgat ctctatatgc
     8581 gcccgcgcac gccgtttgtt gccgggttcg ttggtacatc gaatgttttt gatggactga
     8641 tggcagagaa actttgtggc atgacgggaa gcttcgccct gcggccggaa catatccgcc
     8701 tcaacacccc tggtgaactg caggccaatg gcacgatcca ggcggtgcaa tatcagggcg
     8761 cggcaactcg ttttgaactg aaattgaacg gcggtgaaaa actgctggtg agtcaggcca
     8821 atatgacagg cgaagaactg cctgccacgc tcacgcccgg acaacaggtg ctggtttcct
     8881 ggtcgcgtga tgtgatggtg ccgctggttg aggagaggtg aatggcgatg aacgtattgc
     8941 aatcaccttc acgtccaggt ctgggtaagg tgtccggttt cttctggcgg aatccggggc
     9001 tggggctgtt tttgctgctg cttggcccgc taatgtggtt tggcattgtc tatttcggct
     9061 cgctgctaac gctgttatgg cagggctttt atacctttga cgatttcacc atgtcggtaa
     9121 cgccggaact gacgctggcg aacatccgtg cgctgtttaa tccggcgaat tacgacatca
     9181 ttctccgcac gctgactatg gctgtggcag tcactatcgc cagcgccatt ctggcttttc
     9241 caatagcgtg gtatatggcg cgctatacca gcgggaaaat gaaagcgttt ttttatattg
     9301 cggtaatgtt gccgatgtgg gcgagctaca ttgttaaagc ctatgcctgg acgttattgc
     9361 tggcaaaaga tggcgtggct cagtggtttt tacaacatct tgggctggaa ccactgctga
     9421 cagcgttcct tacattacct gcggtgggcg gcaatacgct gtcaacttcc gggctggggc
     9481 gctttctggt gtttctctat atctggctgc cgttcatgat cctgcccgtg caggcggcgc
     9541 ttgagcgttt gccgccgtca ttgttgcagg cgtcagctga tctcggcgca cgtccacgac
     9601 aaacctttcg ctatgtggtg ttgccgctgg cgatcccggg tattgccgct ggctctatct
     9661 ttaccttctc actcacactg ggcgatttta tcgtcccgca gctggttggc ccgccaggat
     9721 attttatcgg caatatggtt tattcccagc agggggcgat tggcaatatg ccgatggcgg
     9781 cggcattcac cctggtgccg attgttctta tcgcactgta cctggcgttc gtgaaacgtc
     9841 tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc
     9901 ggcgttgttt tcctacattt tcccatcctg ataatcgccg cctatgcgtt taacactgaa
     9961 gatgcggcgt ttagttttcc accgcagggc ctgacgctgc gctggtttag cgtggcagca
    10021 cagcgtagtg atattcttga tgcagtgaca ctgtcactta aagtggcggc gctggcgaca
    10081 ttaatcgcgc tggtgttagg gacgctggca gctgccgcgc tgtggcgacg agactttttc
    10141 ggcaaaaacg ccatttcgct gttactgctg ctgcccattg cgctgccggg cattgtcact
    10201 ggtctggcgt tattaaccgc ctttaaaacc atcaatctgg agccgggatt tttcaccatc
    10261 gtggtcggtc atgcgacttt ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt
    10321 cgccgcacct catggagtct ggttgaggcg tcaatggatc ttggggccaa tggctggcaa
    10381 accttccgct acgtagtgtt gccgaatctc agttcggcgt tactggcagg aggaatgctg
    10441 gcgtttgcct tgtcgttcga tgaaatcatc gttacgacct ttacggcagg tcatgaacga
    10501 acgttaccgt tgtggttgct caatcagctt gggcgaccgc gtgatgtacc ggtaaccaac
    10561 gtggtggcac tgctggttat gttggtaaca accttgccga tcctgggggc ctggtggcta
    10621 acccgcgaag gcgacaatgg tcaataacca ctgatacagg aaaatgctat gcaacataag
    10681 ttactgatta acggagaact ggttagcggc gaaggggaaa aacagcctgt ctataatccg
    10741 gcaacggggg acgttttact ggagattgcc gaggcatccg cagagcaggt cgatgctgct
    10801 gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg
    10861 gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg
    10921 gagtcccgta attgtggcaa accgctgcat agtgcgttca atgatgaaat cccggcgatt
    10981 gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt
    11041 gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct
    11101 atcgcgccgt ggaattatcc gctgatgatg gcagcgtgga aactggctcc ggcgctggcg
    11161 gcagggaact gcgtagtgct taaaccatca gaaattaccc cgctgaccgc gttgaagttg
    11221 gcagagctgg cgaaagatat cttcccggca ggcgtgatta acgtactgtt tggcagaggc
    11281 aaaacggtgg gcgatccgct gaccggacat cctaaagtgc ggatggtgtc gctgacgggc
    11341 tctatcgcca ccggcgagca catcatcagc cataccgcgc cgtccattaa gcgtactcat
    11401 atggaacttg gtggcaaagc gccagtgatt gtttttgatg atgcggatat tgaagcagtg
    11461 gtcgaaggtg tacgtacatt tggctattac aatgccggac aggattgtac tgcggcttgt
    11521 cggatctacg cgcaaaaagg catttacgat acgctggtgg aaaaactggg tgctgcggtg
    11581 gcaacgttaa aatctggtgc gccggatgac gagtctacgg agcttggacc tttaagctcg
    11641 ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg cgaaagcgac agggcacatc
    11701 aaagtgatca ctggcggtga aaagcgcaag ggtaatggct attactatgc gccgacgctg
    11761 ctggctggcg cattacagga cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg
    11821 agtgttacgc tcttcgacaa cgaagaacag gtggtgaact gggcgaatga cagccagtac
    11881 ggacttgcat cttcggtatg gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg
    11941 ctgcaatatg gttgtacctg ggtcaatacc catttcatgc tggtaagtga aatgccgcac
    12001 ggtgggcaga aactttctgg ttacggcaag gatatgtcac tttatgggct ggaggattac
    12061 accgtcgtcc gccacgtcat ggttaaacat taaggataat attgcagatc gtaagagtat
    12121 gagatgatct tacgtactgt cagagcactg atttctctga cagtacgtgc tgcgtgcaaa
    12181 atcactacat caaataaacc agccaaatct catagagata agagtaaaca aaccaacagc
    12241 agcaaagaca tttatcagta ctacagttct actggaaaca ttcatttttg ccacctttta
    12301 tcattgcccc tatgtgtata gtacgggtta agaaaatccg caataatact cattctgaag
    12361 atggataagg gcaagttgct gtttgatgat tttttctcaa actggtgaaa aaacttgatg
    12421 cacgtcaaaa aatgacgcat atttgcgcgc gttttattca tctggctgga cgcccgtaca
    12481 tgtctctcta tcaacacatg cttgtcttct atgcggttat ggcagcaatc gcatttctta
    12541 tcacctggtt tctttctcac gataagaaac gcatccgttt cttaagcgcc tttctggtgg
    12601 gggcaacatg gccgatgagt ttcccggtgg cgctgttgtt ttcactgttt taaatcttac
    12661 tcactggctt gacggcacca cgcagactta tatcatttgg atgaatcgat aaatttcaca
    12721 agtggctaag gagaaagtat gtcgcatctg gatgaagtca tcgcgcgcgt ggatgccgcg
    12781 attgaagaga gcgtcattgc ccatatgaac gaattattaa tcgccctgag cgatgacgcg
    12841 gagttaagtc gggaagatcg ctacacccag cagcagcgtt tgcgcaccgc tattgctcat
    12901 cacggtcgca agcataaaga agatatggaa gcgcgccatg aacagttaac caaaggcggc
    12961 accatcctgt gattaaaatg agcgtctggc taccaaccag gcgccaacca caagtaaaat
    13021 agcgccacaa attgggccga acaacgcccg tagtggtacg ccatgactgc ggaagatatc
    13081 cagaaccaga ccgccaatca gctggctggc gaccagtact gcaatagttg tcgccgctcc
    13141 cacattctga tatccgctga tactggcgaa gacaaaaaac gatcccagca agccaggtat
    13201 aagtgtccac cagcgcacgc tggacaccag ttcgccaaac cccgccatac cttgtttaaa
    13261 ccataaaata gaaacaaaga gcacaatccc caccagtgag ttcagcaaca tggcgatgag
    13321 aatggtagag gaggtctggg tgatgcgcac catcagcgtg ttctggacta ccagaccaat
    13381 tccggcggca atcaaaaagg caagggtgag cgactgattc atccaatcgc gtccggttca
    13441 gtgcgttcgt cgagttgcaa ctgcataaat gtcagatcca gccagcgccc aaatttagtg
    13501 cccacctgcg gcatttgtgc ggtgacgaca aatcccagcg actggtgcag atgcagcgag
    13561 gcctgatttt gcgattcgat cccggcgacc atgacatgct tcccgcaatc tcgcgcttca
    13621 tcaatcaatc ggcttaacaa tttacgcccc agacctttgc cctgatgatc gggatggaca
    13681 taaaccgaat gttccacggt atggcgaaaa ccatcgaaac tacgccagtc gccaaatgag
    13741 gcatatcccg tcactacgcc atcttcttca cttaccagca ccggataacc tgctatggtc
    13801 cgcgcttcaa accaggcaat gcggttatct gcatccaccg tttggtcatt ccagatagcc
    13861 gccgtataca acacggcgtg gttataaatt tccgcaatgg cagcacagtc ggctttgcgg
    13921 gcaaaacgga tggacatgtc tggcctcaat aaaataatga tgatgatgtt atcaatacta
    13981 tcgaaaacag cccgaagcaa cggattcctc gatgaaatta ttgcgtgttg cagagggggt
    14041 gtgacggctg caaacaaaac caatcctgcg agcctgctac tattgatgga ttcatgccaa
    14101 aagcacagga gagcattatg gggcaacaaa agcagcgtaa tcgacgttgg gttctggcct
    14161 cgcgtccaca tggcgcaccg gttccggaga atttccgtct tgaagaagat gatgtcgcca
    14221 caccgggtga aggacaggtg ttactgcgca cagtttattt gtccctggac ccgtatatgc
    14281 gtggacgtat gagcgatgag ccatcttatt caccgccggt tgatattggc ggcgtgatgg
    14341 tcggcggtac ggtgagccgt gtcgaggagt cgaatcatcc tgattatcag tctggcgact
    14401 gggtgctggg ctacagtgga tggcaagact atgacatatc cagtggtgat gatctggtga
    14461 aacttggcga tcatccgcaa aatccatcgt ggtcgctggg tgtgctaggg atgccaggct
    14521 ttaccgctta tatgggccta ctggatatcg gtcagcctaa agagggcgaa acgttggtgg
    14581 tagctgcggc gacaggacca gtgggggcga cggtggggca aatcggcaaa cttaaaggtt
    14641 gcagagtggt gggggtagcc ggtggcgcgg aaaaatgccg ccatgctacc gaggtgttag
    14701 gcttcgatgt ttgccttgat caccacgcgg acgattttgc agaacaactg gcgaaagcgt
    14761 gcccaaaagg tattgatatc tattatgaaa acgtgggcgg taagatattt gatgcagtgc
    14821 taccattact taatacatct gcgcgcattc ccgtttgtgg cttagtgagc agctataacg
    14881 ctacagagct accacccggt ccggatcgtt tacccctgtt gatggcaacg gtgctgaaaa
    14941 aacgtattcg cttgcaaggg tttattatcg ctcaggatta tggtcaccgc atccatgagt
    15001 ttcagaagga gatggggcaa tgggtgaaag aggataaaat ccactaccgc gaagaaatta
    15061 ctgacggttt agagaatgcg ccacagacgt ttatcggcct gctgaagggt aaaaacttcg
    15121 gcaaagtggt gatccgcgtg gcgggtgatg attaaagttt caacggcggc gtaagccgcc
    15181 ataaaaatcc aggcaaaaag cttattttca gctttaatta actaaccaca tcattgttct
    15241 gctgctaata acaacaaagg tgagtggttt atgattattc actttaatac accaggtgaa
    15301 ttccttctgc catgcaggca gggttggaca gaaaacacgc gaaggaatta aaatgcctgg
    15361 aacggaaaaa atgaaacatg tcagtttgac tctgcaggtt gagaacgacc tgaaacatca
    15421 gcttagtatt ggcgcactaa aacctggcgc acgcctgatt actaaaaatc tggcggagca
    15481 attaggtatg agtattacac ctgtgcgtga agcattatta cgtctggttt cggtgaatgc
    15541 gctttctgtc gcaccagcgc aagcatttac agttccggaa gtggggaaac gtcaattaga
    15601 cgaaatcaat cggatccgct acgaactgga attaatggca gttgctctgg ctgttgaaaa
    15661 cctcaccccg caagacctgg cggaactcca ggaattgctc gagaaattac agcaggcgca
    15721 agaaaagggc gacatggaac aaatcattaa tgtaaacagg ctatttcgct tagcgattta
    15781 tcatcgctca aatatgccca tcctgtgtga gatgattgag caactgtggg tcaggatggg
    15841 acctggttta cattatctct atgaagcgat taatccagcg gaattacggg agcatataga
    15901 aaactatcat ctgttactcg ctgcgttaaa agcaaaagac aaagagggat gcagacattg
    15961 tcttgctgaa attatgcaac aaaatattgc tattttatac cagcaataca atcgttaaag
    16021 taaatgcccg gcaaataccg ggcatcaagg taattactca aatcgccatg cgatattcac
    16081 gccgacacca taatttcgtc cgggcgcagg ttcgtaatat cgcccgtttg actcattgac
    16141 aatgacagaa ccaacgtatt ctttatcgaa taaattatcg acacgaccaa ataaatccac
    16201 agttaaattg tggtaattgt atttataccc ggtgaataag ccgatgagag tataagacgg
    16261 cgccttggcc gtattttcat catctgccat aatatcgccc atataacgcg cttccgtgcc
    16321 tgcataccag ccatcttccg gtacataacc tatcgacgca aagcccatat tacgggcgat
    16381 ccctggcatc cgattaccgt tacaatcctg ttcattgcaa acattgctgc gataggtcgc
    16441 atcaagccag gtccaggacg cgtttacgcg aaaatctccc gcgaaacgtt gatcccatgc
    16501 cagttcagcg ccttgacgac gggtctttcc agcatttttg taagtcgtac gcccaccgct
    16561 actgctatcg acaacaattt catcatcggt gtcggtctgg aacaatgcga gactaagcag
    16621 cccatcacca atacgcgttt tactgccgat ctcaattgta tcgttggtgg atggttttaa
    16681 accaaagttc ataccgcttt gcccatcagc gcgataagac agctcattaa tcgtcggcgt
    16741 ttcaaaacca cgcccggctg ccagatagat attccaggca tcggtcattg catattttaa
    16801 cgatccggct ggtagccatt tgtgataact ggcatcaccg ctgtcatcgc cgttacccgg
    16861 agtaacgtaa tggtcgttgg aatcaaacca cacggagctg tagcgcacgc cagcatccag
    16921 cgacagtttt tcgctcagct gccactgcgt ctgtaaatag ggatcgatgt tccacatcag
    16981 attgcgttcg tcgcgacgca actcaccttt ttgcccgtac tccggcatgc cgctattcag
    17041 gcggaagtta ttgtagccct tgcggttttc actcatgttt tcgtagttca ggccggtagt
    17101 gaacgtgacc ggaacgccca gttcaccacg gtgtgtccag cggctgtcta ttccctggta
    17161 atggcgttgc agggtaatca cgccgcccgc atgtgacggg ttaagttgtg gcgccatggg
    17221 tattgactgg tactgggtcg tttctcgctc tccggcatac atcatcacac tcatatcatc
    17281 ccgcgaactc aggctacgtt catagcgcaa cccagcctga gtttgcttga tggtttttcg
    17341 cgtgtcgtac tgttctgcac gaggcgcttg ttgtggattc gccttccatt ctgctttggt
    17401 tagcccaccc gggtcatctg ctttgatatc cacactattg aaaatcaggc ttaatttgct
    17461 ggcttcatca atgcgtacgc ccagtttggc attggctaaa tttttctgtg cgccactatg
    17521 gtcacgatag ccgtgggtcg taaaacgcgt ggttgagacg gtgtaatcaa catcgccggg
    17581 ctgtgtgccg tctcccgttg cgcccgttgc tttcagccca tagcgccagc tgccaaaact
    17641 gccgtagtaa ctactggctt caatggttgg tggctgttgt ccggtctggg tggtgacatt
    17701 cattacaccg ccagacgcgt tgccatacag ggcagagaag gggccacgca gcacttccac
    17761 attttgcaca ctgcttaaat cgatgttgga tgtttgccct tgcccgtcgg gcatggtggc
    17821 gggaataccg tccacataca ggcgaatacc gcgaattccg taagtggaac gggagccaaa
    17881 tccgcgaatc gacagctgta aatcttgcgc atagttctgc cggttttgta cctgcaaacc
    17941 aggcacgccg gtcagtgatt cggacaagtt aatgcgcggt gttgccaggc gcatctcctc
    18001 gccatccacc acgcttactg ctgcaggggt atccagttct gaaaccacct gcggcgcggc
    18061 actgacaatc atggtctgtt catcagcggc aaaaacaacg ggggaaagga caagcagtgc
    18121 gggcaaaacg gtctgtcgga cggaaaaaat cttcatgaaa aaagccaggt taagaatggg
    18181 aaaacgccgt catggtaatg aaattgtaaa tttatggaaa atgaaacggc acaatacgtt
    18241 aagtaattga gaaaattgta gtcgtaacgg caagaaatgc tccacatttg agaaaataat
    18301 gattaccatt cccatttata acaagagcgt aacgatgatt acgcttagcg aagcattgtg
    18361 aagcagcaaa aatatcggtt catcaaaggg agtcgtcatg catttacgtc atctgttttc
    18421 atcgcgcctg cgtggttcat tactgttagg ttcattgctt gttgcttcat cattcagtac
    18481 gcaggccgca gaagaaatgc tgcgtaaagc ggtaggtaaa ggtgcctacg aaatggctta
    18541 tagccagcaa gaaaacgcgc tgtggctcgc cacttcgcaa agccgcaaac tggataaagg
    18601 tggcgtggtt tatcgtcttg atccggtcac tctggaagtg acgcaggcga tccataacga
    18661 tctcaagccg tttggtgcca ccatcaataa cacgactcag acgttgtggt ttggtaacac
    18721 cgtaaacagc gcggtcacgg cgatagatgc caaaacgggc gaagtgaaag gccgtctggt
    18781 gcttgatgat cgtaagcgca cggaagaggt gcgcccgctg caaccgcgtg agctggtagc
    18841 tgatgatgcc acgaacaccg tttacatcag tgttattggt aaagatagcg tgatttgggt
    18901 cgttgatggc gagaatatca aactgaaaac cgccatccag aacaccggta aaatgagtac
    18961 cggtctggcg ctggatagca aaggcaaacg tctttacacc actaacgctg acggcgaatt
    19021 gattaccatc gacaccgccg acaataaaat cctcagccgt aaaaagctgc tggatgacgg
    19081 caaagagcac ttctttatca acattagcct tgataccgcc aggcagcgtg catttatcac
    19141 cgattctaaa gccgcagaag tgttagtggt cgatacccgt aatggcaata ttctggcgaa
    19201 ggttgcggca ccggaatcac tggctgtgct gtttaaccca gcgcgtaatg aagcctacgt
    19261 aacgcatcgt caggcaggta aagtcagtgt gattgacgcg aaaagctata aagtggtgaa
    19321 aacgttcgat acgccgactc atccaaacag cctggcgctg tctgccgatg gcaaaacgct
    19381 gtatgtcagt gtgaaacaaa aatccactaa acagcaggaa gctacccagc cagacgatgt
    19441 gattcgtatt gcgctgtaat agatatggcg agggtgaaaa cggagagact tgcctgactt
    19501 ttatcacgcc gcatgcggca caccttcgtc agtaatctaa atttgcccgg cacgtagccg
    19561 ggcaattttg caattagctc gccgtttcag gcttaaacac aatttcctgt ttttcttcat
    19621 cttcctcgac gactggcgca gtgctgtgaa tttcagcaac gcgtttgcgc acaccaaacc
    19681 agcctataac cagcagaata ccgataatcg gcagcgccgc gatagtataa gtcccgttcg
    19741 ggtaatcgaa cgccatcagc acaaggacac tcagtaaaaa cagtaatgtc agccaggaag
    19801 tgaagggcgc gccaggcagt ttaaaactga catccgctgc tttgccttct ttaatcgctt
    19861 tacgcaggcg catctggcac acgatgataa acgcccatga agcgatgatt cccagcgacg
    19921 cgaagttcaa cacaatctca aatacgcgcg acggcaccag atagttgagg aatacgccga
    19981 cgacatacac aactagtgtc gccagaatcc cggcatacgg cacatgctga cgactcattt
    20041 tcgccataaa actcggtgcg gaaccgccca tcgccattga gcgcagaata cgtccggtgc
    20101 agtacagacc tgaattcagg ctggagaggg cagcggtcag caccacaatg ttcataatgc
    20161 tgccgatata tggcacaccc agtttagaga aaaacgtcac gaacggactt tgccccgcct
    20221 gatacgcgct ccacggcaat aacataacca gcaacaccac ggagccgacg taaaacaggc
    20281 caatacgcca aatcacactg ttaatcgctt taggcaccat ggtctgcgga tctttacatt
    20341 ctccggcagc tgtacccacc atttcaatgg aggcaaaagc aaacactacg ccctgaatca
    20401 acaccagcgc aggcagcaaa ccgtggggga agaagccgcc attatcggtg attaaatgaa
    20461 agccagtggt gttgccatcc agcggctgac cactaccgag gaacactgta cccacgacca
    20521 aaaaggtcac aatggcgagc actttaataa gcgcaaacca gaactccatc tccgcaaacc
    20581 atttcacacc gatcatattc atggtgccaa cgatggtaag tgcagcgagc gcaaagaccc
    20641 actgcggcac gccgccaaac gcaccccagt aatgcatata cagagcgacg gcggtaatat
    20701 caacaatccc ggtcatcgcc cagttgatga agtacatcca accggcaaca taagcggctt
    20761 tctcacccag aaactcacgg gcataggaga caaagctgcc actggaaggg cgatgcaata
    20821 ccagctcacc caacgcgcgc agaataaaaa acgaaaacag gccgcagatt aaatagacca
    20881 gtgccagcgc cggccccgcc atttgcagtc gggctcccgc gcctaaaaac aaaccagtac
    20941 caatcgcacc gccaatggcg atcatctgca cctgacgatt gcccattgct ttgtgatatc
    21001 cctcttcgtg ggcattaagc cagcggcgtt tcgcagcgtg ttgctctgaa gtgtcggtgt
    21061 tgtgtttact cattgcgctc cctgattgct ttaatgaaaa aagtctttta tgtcgccatg
    21121 aacaatgttt attcatcctg gcgatgatag ttatcaccat tattctttat gggtataaaa
    21181 ctatcggcgc agcatcctac ccgttatcga taaacgatgc aaaacatccc cttacaatcc
    21241 tgaaggggat taatacaact gacgaaaaaa tgacaaatcc ttttgctggt taacctgtgt
    21301 actgtcctac acttaatctt taaaagattg tgaggggcat atgattaagg tgtatggcgt
    21361 acccggatgg ggctctacaa tcagtgagct gatgctaaca ctggctgata ttccttatca
    21421 gttcgttgat gtcagtggtt ttgaccacga gggagcttca cgcgagttat tgaaaaccct
    21481 gaacccgttg tgtcaggttc cgacactggc attggaaaat gacgagatta tgacggagac
    21541 agcggcgatt gcattgatgg ttctcgatcg tcgcccggac cttgcgccac cggttgggcg
    21601 cgccgaacgt cagcagtttc aacggctatt ggtctggctg gttgctaatg tctatccaac
    21661 attcactttc gccgattacc ctgagcgttg ggctcctgac gcacccgaac agttaaagaa
    21721 gaatgttatt gaatatcgta aatcgcttta tatctggctg aattcacaat tgaccgctga
    21781 accttatgcg tttggtgagc aactaacgct ggtggactgt tatctttgca ctatgcgcac
    21841 atgggggcct gggcatgaat ggtttcagga taatgccacg aatatcagtg cgattgctga
    21901 tgctgtatgt cagttaccaa cgttacaaga aatactaaaa aggaatgaaa ttatttgagt
    21961 gggataccag aagattatgt tatttattat tcagtaactt tatccacaat gatgggcgta
    22021 attaattaga ttcatgttat gttttttaat ttatatcact ctccctttca ttcagcttgt
    22081 ctatttcatt tcctctgaaa agaaactaac tattcacatt gtgaagatgt ttcacttgtt
    22141 atcacaggca ttctataatc tcaaaatg
//
LOCUS       DAZGBC010000080        21579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGBC010000080 DAZGBC010000000
VERSION     DAZGBC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.080"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            481..762
                     /gene="drpB"
                     /locus_tag="ACHK1J_002874"
     CDS             481..762
                     /gene="drpB"
                     /locus_tag="ACHK1J_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO7912117.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            802..1497
                     /gene="yedJ"
                     /locus_tag="ACHK1J_002875"
     CDS             802..1497
                     /gene="yedJ"
                     /locus_tag="ACHK1J_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO7912118.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALDVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPHTMQTIRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1564..2982
                     /gene="dcm"
                     /locus_tag="ACHK1J_002876"
     CDS             1564..2982
                     /gene="dcm"
                     /locus_tag="ACHK1J_002876"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO7912119.1"
                     /translation="MQENILVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2963..3433
                     /gene="vsr"
                     /locus_tag="ACHK1J_002877"
     CDS             2963..3433
                     /gene="vsr"
                     /locus_tag="ACHK1J_002877"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO7912120.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDS
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3422..4342)
                     /gene="yedA"
                     /locus_tag="ACHK1J_002878"
     CDS             complement(3422..4342)
                     /gene="yedA"
                     /locus_tag="ACHK1J_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO7912121.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4516..5433
                     /gene="yedI"
                     /locus_tag="ACHK1J_002879"
     CDS             4516..5433
                     /gene="yedI"
                     /locus_tag="ACHK1J_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO7912122.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5512..5694
                     /gene="yodC"
                     /locus_tag="ACHK1J_002880"
     CDS             5512..5694
                     /gene="yodC"
                     /locus_tag="ACHK1J_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO7912123.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSNGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPVEEV"
     gene            5937..7631
                     /gene="dgcQ"
                     /locus_tag="ACHK1J_002881"
     CDS             5937..7631
                     /gene="dgcQ"
                     /locus_tag="ACHK1J_002881"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO7912124.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELAMLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7628..8443)
                     /gene="yedP"
                     /locus_tag="ACHK1J_002882"
     CDS             complement(7628..8443)
                     /gene="yedP"
                     /locus_tag="ACHK1J_002882"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO7912125.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8741..8968)
                     /gene="yodD"
                     /locus_tag="ACHK1J_002883"
     CDS             complement(8741..8968)
                     /gene="yodD"
                     /locus_tag="ACHK1J_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO7912126.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQHDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9131..9319
                     /gene="dsrB"
                     /locus_tag="ACHK1J_002884"
     CDS             9131..9319
                     /gene="dsrB"
                     /locus_tag="ACHK1J_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO7912127.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9363..9986)
                     /gene="rcsA"
                     /locus_tag="ACHK1J_002885"
     CDS             complement(9363..9986)
                     /gene="rcsA"
                     /locus_tag="ACHK1J_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO7912128.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10276..11061)
                     /gene="fliR"
                     /locus_tag="ACHK1J_002886"
     CDS             complement(10276..11061)
                     /gene="fliR"
                     /locus_tag="ACHK1J_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO7912129.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11069..11338)
                     /gene="fliQ"
                     /locus_tag="ACHK1J_002887"
     CDS             complement(11069..11338)
                     /gene="fliQ"
                     /locus_tag="ACHK1J_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO7912130.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11348..12085)
                     /gene="fliP"
                     /locus_tag="ACHK1J_002888"
     CDS             complement(11348..12085)
                     /gene="fliP"
                     /locus_tag="ACHK1J_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO7912131.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12085..12450)
                     /gene="fliO"
                     /locus_tag="ACHK1J_002889"
     CDS             complement(12085..12450)
                     /gene="fliO"
                     /locus_tag="ACHK1J_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO7912132.1"
                     /translation="MNNHATVQSSAPISAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12453..12866)
                     /gene="fliN"
                     /locus_tag="ACHK1J_002890"
     CDS             complement(12453..12866)
                     /gene="fliN"
                     /locus_tag="ACHK1J_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO7912133.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADTVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12863..13867)
                     /gene="fliM"
                     /locus_tag="ACHK1J_002891"
     CDS             complement(12863..13867)
                     /gene="fliM"
                     /locus_tag="ACHK1J_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO7912134.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13872..14336)
                     /gene="fliL"
                     /locus_tag="ACHK1J_002892"
     CDS             complement(13872..14336)
                     /gene="fliL"
                     /locus_tag="ACHK1J_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO7912135.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14441..15568)
                     /gene="fliK"
                     /locus_tag="ACHK1J_002893"
     CDS             complement(14441..15568)
                     /gene="fliK"
                     /locus_tag="ACHK1J_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO7912136.1"
                     /translation="MIRIAPLITADVDNTTLSGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSTQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLIAPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15565..16008)
                     /gene="fliJ"
                     /locus_tag="ACHK1J_002894"
     CDS             complement(15565..16008)
                     /gene="fliJ"
                     /locus_tag="ACHK1J_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO7912137.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAATRKPE"
     gene            complement(16027..17400)
                     /gene="fliI"
                     /locus_tag="ACHK1J_002895"
     CDS             complement(16027..17400)
                     /gene="fliI"
                     /locus_tag="ACHK1J_002895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO7912138.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISPE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17400..18086)
                     /gene="fliH"
                     /locus_tag="ACHK1J_002896"
     CDS             complement(17400..18086)
                     /gene="fliH"
                     /locus_tag="ACHK1J_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO7912139.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEETEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18079..19074)
                     /gene="fliG"
                     /locus_tag="ACHK1J_002897"
     CDS             complement(18079..19074)
                     /gene="fliG"
                     /locus_tag="ACHK1J_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO7912140.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19067..20725)
                     /gene="fliF"
                     /locus_tag="ACHK1J_002898"
     CDS             complement(19067..20725)
                     /gene="fliF"
                     /locus_tag="ACHK1J_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO7912141.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20940..21254
                     /gene="fliE"
                     /locus_tag="ACHK1J_002899"
     CDS             20940..21254
                     /gene="fliE"
                     /locus_tag="ACHK1J_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO7912142.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
BASE COUNT         5139 a         5504 c         5693 g         5243 t
ORIGIN      
        1 gaattatgaa ttctaaatat aaatattcat gaaaattata aatgtatatc aatatgttat
       61 gagttgagcg gaaagatagg gggtatcaat gatggttgcg ccaaacacct ggtgtgatca
      121 cgcgcacatt aatgaaggca taaaaaaaag ccggcatcat gccggcccaa aaaatataaa
      181 caagacgata aaaggctaat aacggaggca tcatgacaca gaataataaa aaggtataat
      241 tgtattcagt gctaatatat tgattaaatg aattaataaa gccgatatca aagataaata
      301 aaatcgctca aaacgatttt tcctgtattc actgccgttg cgcaaaaatt atctatttgt
      361 tcaaaaaatg agtgagtctt gactggctca tccaatgtgg aaaaatgtga cttttatcac
      421 ataatagtac taagtctgaa ttttccgggt tatctcaaaa tggaatacgg ttcgacaaag
      481 atggaagaga gactctctcg cagccctggc ggcaaactgg ctttgtgggc tttctataca
      541 tggtgtggct atttcgtctg ggcaatggcg cgttacatct gggtgatgag ccggatcccc
      601 gatgcccccg tgtcgggatt tgaaagcgat ttaggctcta ccgcgggaaa gtggttaggg
      661 gcactggtcg gatttttatt tatggctctg gtcggggcat tgttaggcag cattgcctgg
      721 tacacccgac ctcgtcccgc acgtagcaga cgctatgaat aaaaattgct aaggtggcga
      781 ctctttggca acggagcgcg tatggactta caacactggc aggcacagtt cgaaaactgg
      841 ttgaaaaacc atcatcagca ccaggacgcg gcccatgact tgtgtcattt tcgccgcgtc
      901 tgggctacgg cacagaagct ggcggcagat gacgatgtcg atatgttggt gattttaacc
      961 gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc atccgcaacg gcagcgttct
     1021 tcaattctgg cggcagaaga gacgcgtcgc ctgctccgtg aagagttcga gcaatttccg
     1081 gcggagaaaa tcgaggccgt ttgtcatgcc attgccgctc acagtttcag cgcgcaaatc
     1141 gcccccttaa caacggaagc taaaatcgtc caggatgcgg atcggctgga agccctgggg
     1201 gcgattggcc tggcgcgcgt gtttgccgtt tcaggagcat tggacgtggc gctgtttgat
     1261 ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata aacgttacgc gctggatcat
     1321 ttccagacta agttgctgaa gctaccgcat accatgcaaa ccataagagg caagcagctg
     1381 gcgcagcaca acgcgcaatt tttagtcgag tttatggcga agctcagtgc cgaactggcg
     1441 ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt tttcacctgc gggctgagcg
     1501 cgtggcccta aatggctgta attatgttaa cctgtcggcc atctcagatg gccggttaaa
     1561 tctatgcagg aaaatatatt agtaaccgat tcatacagca ccgggaatgc cgcacaggca
     1621 atgctggaga aactgctgca aatttatgat gttaaaacgc tggtggcgca gcttaatggt
     1681 gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg cgctggcgaa tgactcggca
     1741 tggcaccgtt taagtgagaa agagttcgcc catctgcaaa cgttgttacc caaaccaccg
     1801 gcacatcatc cgcattatgc gtttcgcttt atcgatctat ttgccggaat tggcggtatc
     1861 cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta ccagcgaatg gaacaaacat
     1921 gcggtacgca cttataaagc caaccattat tgcgatccgg cgacgcatca ttttaatgaa
     1981 gatatccgcg acatcaccct cagccataaa gaaggcgtga gtgatgaggc ggcggcggaa
     2041 catattcgtc aacacattcc tgaacacgat gttttactgg ccggtttccc ttgtcagcca
     2101 ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc gggcgcacgg ttttgcctgc
     2161 gatactcagg gcacgctgtt ctttgatgtg gtgcgcatta tcgacgcgcg tcgtccggcg
     2221 atgtttgtgc tcgaaaacgt caaaaacctg aaaagccacg accagggtaa aacgttccgc
     2281 atcatcatgc agacgctgga cgaactgggc tatgacgtgg ctgatgcaga agataacggg
     2341 ccggatgatc cgaaaatcat cgatggtaaa cattttctgc cgcagcaccg tgaacgcatc
     2401 gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg attttactct gcgtgatatc
     2461 agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc tgctggaccc gatggtcgag
     2521 gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct atcgttatgc gaaaaaacat
     2581 caggcgcgcg gtaacggctt cggttatgga atggtttatc cgaacaatcc gcaaagcgtc
     2641 acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg aaattttaat cgatcgcggc
     2701 tgggatatgg ccacgggtga gaaagacttt gacgatccgc tgaatcagca acatcgtcca
     2761 cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt ttgaagcgcc gggagaagcg
     2821 aaattccgca ttccggtttc ggacactcag gcctatcgcc agttcggtaa ctcggtggtc
     2881 gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa aaatcaaaca ggcggtggcg
     2941 ttgcgtcagc aagaggcaca acatggccga cgttcacgat aaggccactc gcagcaaaaa
     3001 tatgcgcgcg attgccacgc gtgatacggc gattgagaag cgcctcgcca gtctgttaac
     3061 cgggcagggc ctggcgtttc gcgttcagga ctccagtctg cccggacgcc cggattttgt
     3121 cgttgatgaa tatcgctgcg tgatatttac ccatggctgc ttctggcatc atcatcactg
     3181 ctatctgttt aaagtgcctg cgactcgaac cgagttctgg ctggagaaga taggtaaaaa
     3241 tgttgagcgc gatcgccgcg atatcagtcg cttgcaggaa ctcggctggc gcgtattgat
     3301 tgtctgggag tgcgcgttac gtgggcgcga gaagctgacg gatgaagcgc ttagcgagcg
     3361 tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag atcgacacgc aggggataca
     3421 tttactcgct tgatgcgtcc tgaataactg gcgcaactac gggttttgcc ggaaagagat
     3481 attttcccaa cgtgaccagt accaccgcga agacaattac gccgagcgcc agccattcaa
     3541 tcttcgacag tgtttctcca cccagtcccg tacccagcaa gaccgcgacc accgggttaa
     3601 cgtaggcgta gctggtggcg agagccggac tgacgttgcg gattaaatac atataagcgt
     3661 taatggcgat aatcgaacca aacagcgcca gatagccgac cgcaaggaag cctgaaaggg
     3721 aagggagcgc cgtcagtttt tcacccgcaa tcatcgacgc gatcattaac accacgcctg
     3781 ccgccagcat ctcaatcgca cccgccatca tccctacagg taaggtaatg cgcgagccat
     3841 aaactgagcc aaacgcccag ctaatcgagc cgattaaaat cagaatcgcg ccccacggat
     3901 tgccgcttaa atttccaccg ctattgagca tgatgattcc ggcaagccca atggcaatac
     3961 ccacccattc cagtttgcgc gttttaatgc caaacaggcg gctgaagcac agggtaaaga
     4021 ggggcacggt tgcaaccact acggcggcga tgccggaagg aacattttga tgttcggcaa
     4081 ccgtcaccat gccattaccg acagccagca ataacaggcc aatcagcgcg gcattgagca
     4141 gcggacgtag cggggggagc ttgtgtccgc gcagtagcaa aaatgccagc aataaaatac
     4201 cggctgccag gaatcgaacg cccgccatca ttaatggagg ccagctttcc acgccaatcc
     4261 gaatgacaaa ataggttgag ccccaaatga tatacaacgc aaacagcgcg ccaaaaagcg
     4321 gtaataactg gcggaaacgc ataatccctc acggtggaaa taaaaaggtg gttcatagta
     4381 aacgtgaaaa tcattctgct ggcgagagat ataattgcac ttgattgtta aaaaaaatgt
     4441 tgacctgtga agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa
     4501 taaaaatgag agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc
     4561 gccacactgc tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt
     4621 gtattagggg atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg
     4681 gaacttcccg tggtctgggg cgtggcgaaa ggatcgctga tcaataaagt gattctggtg
     4741 ccactggcgc tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt
     4801 ggtggcgcgt ttctctgctt tgaaggggta gagaaagtgc tgcatatgct ggaggcgcgt
     4861 aaacataaag aagatccggc gcagagtcag cagcgtctgg agaagctggc ggcgcaggat
     4921 ccgctgaagt ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct
     4981 gccgaaatcg tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg
     5041 ctggtgcttt caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt
     5101 atcgttaaga ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag
     5161 gcattaggta aaggattatt gattatcgcg ccctggctga tgaaagcgtt atcgattgtc
     5221 ggcacgctgg cgatgttcct cgtcggcggc gggattgtgg tacatggtat tgcgccgctg
     5281 catcacgcca ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg
     5341 actgttttaa atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa
     5401 gccgtagcga aaatgcgcgg tcaggcacat taatactcgc acaggctatt atgcaaaatt
     5461 cgccatcttc aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt
     5521 atggttagtg aggaagttac ggttaaagag ggcggcccgc ggatgattgt cactggatac
     5581 tccaacggta tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt
     5641 catgaaaccg agcttgttcc gggggagggg agtcgtcctg tggaagaagt ttgaaaggga
     5701 atcattaaca ccaggccgga tgtggcgctc acgtcgcatc cggtcgggtg tgccagtgtc
     5761 tgcactgaat tgcctgatgc gacgctatcg cgtcttatca tgcctacgtt ataaaacaaa
     5821 actatcgatg tctgtcgcgt cttatcatgc caccattata aaattatcgc tgttttgcgg
     5881 tacgctatcg ccaggttatg ccagaatcat aaaaaagcag gttgggagtc gtcagggtgc
     5941 agcacgagac aaaaatggaa aaccagagct ggttgaaaaa actcgcacgc cgcctggggc
     6001 ctggtcatgt cgttaatctc tgctttatcg tggtattgct tttttccacc ttgctcacct
     6061 ggcgtgaagt ggtagtgctg gaagatgcct atatctccag ccagcgtaat catctggaaa
     6121 acgtagccaa cgcgctcgat aagcatttgc agtataacgt cgacaaactg atctttttgc
     6181 gtaatggcat gcgcgaagct ctcgtagcgc cactggattt cacctctctg cgtgatgctg
     6241 taaccgagtt cgaacagcat cgcgacgagc acgcctggaa aatcgaactc aaccgacggc
     6301 gcaccctgcc agtcaatggc gtgtcggatg cattagtcag cgaggggaat ctcctgtctc
     6361 gcgaaaatga aagcctcgac aatgaaatta ccgctgcact ggaagttggt tacttgctgc
     6421 gactagcgca caactcctcg tcgatggttg aacaggcgat gtatgtctcg cgtgccggat
     6481 tttacgtttc gacgcagccg accttgttta cgcgcaatgt cccgacgcgt tattacggct
     6541 atgtcaccca accctggttt atcggccatt cacaacgaga aaatcgtcac cgcgcggtac
     6601 gctggttcac ttcgcaaccg gaacacgcca gcaatactga accgcaggtt accgtcagtg
     6661 ttccggtaga cagtaataac tactggtatg gcgtgctggg gatgagtatt cccgtgcgta
     6721 ctatgcagca atttttaaga aacgccatcg ataaaaacct cgatggtgag tatcagctct
     6781 atgacagtaa gctgagattt ttgacctctt ccaatcctga ccatccaaca gggaatattt
     6841 ttgatcctcg tgaactggcc atgctggcgc aggcaatgga acatgacacg cggggcggca
     6901 ttcgtatgga cagtcgctat gttagctggg aacgtctgga ccatttcgac ggtgtgctgg
     6961 tgcgcgtcca tacgttaagc gaaggcgtgc gcggcgattt cggcagtatc agcattgcat
     7021 taaccctgct gtgggcgctc tttaccacca tgttactcat ctcctggtat gtgattcgcc
     7081 ggatggtcag caacatgtat gttctgcaaa gctcgttgca gtggcaggcg tggcacgaca
     7141 ccttaacgcg tttatataac cgtggcgcac tgttcgaaaa agcccgtccg ctcgcgaaat
     7201 tgtgtcagac gcaccaacat cctttttctg tcattcaggt cgatcttgac cattttaaag
     7261 cgattaatga ccgctttggt catcaggcgg gcgaccgtgt tctttctcat gctgccggat
     7321 taattagcag ttccttgcgt gcgcaggacg ttgccgggcg ggtcggtggt gaggagtttt
     7381 gtgtgattct gccaggcgcg agtctgacgg aggctgcgga agtcgcagaa cgtattcgcc
     7441 tgaagttaaa tgaaaaagag atgttgattg ctaagagtac gacgatacgc atcagtgcct
     7501 cgttgggggt aagtagcagc gaggaaaccg gtgattatga ttttgaacaa ctccagtcac
     7561 tggccgaccg tcggctttat ctcgctaaac aggctgggcg taatcgggta ttcgcgagcg
     7621 ataacgctta acgggcggag aaaaaatggt ccagcccttc acgccatcct tccggtcctt
     7681 cacgctgcgt tcgccagacg cgggccggat cctcatcatg cagatgcacc ccttcacggt
     7741 ttagcccttt cacaatcacc gcgtaatcca ttacctccag taagggcgca tcgtttggcc
     7801 catcgcccag gccaagtgtg gttgggcgtt tgcctgacaa ttgttgatag gtcgcgataa
     7861 tccagttggc agcctgatct tttccggcag aggcatccag tacgtgccag aagcgcgcac
     7921 cttgcataaa ctgtaagccc agttcgttca gacgagcggt aaattgtgcc atacgctcgt
     7981 cactgtcgcg ccaaattagc gttaccgacg cctcatgaag ctgcgtcagc gccgcctggc
     8041 tacggcttaa tcccgtccat tcggcgatgg ttgcatcgtc gacatcatca aaagtcgtga
     8101 atttaaaatg ttctttctcg cgtagcgtat ttaaaacctg gctgatttcg ccatggctaa
     8161 tacctgagat gatgcgtgga aaaccgtcta tctcctgcca ttgctcagca agctggatca
     8221 ctgcgccatt ttcggcaatc agcggtaaac cttgtagccc caacgttttt tgcaagtaca
     8281 gcatttccgc tgatgtttta ctgctacaga gaatgacggg aacatttgct tcgcgtaaac
     8341 gggtgagcca gggggctgcc ggttgccagt cataactatg actgtccagc agggtgccat
     8401 caagatcgct aaaaaccagt agtggttgtt gaattgaaaa catagttgcg cagtactccc
     8461 cttaccagga tgattctcaa aacagcacaa attatcaaag atgatttttt cgggagcgat
     8521 cccgctgacg ccagagccac caaggggtga cgtgcgtcac atttctattc ataagtagcg
     8581 ttaatcattc atatggcgaa tattttcttg tcagcgaaaa aaattgcgga taaggtgatg
     8641 aacacatcag atttcctggt gtaacgaatt ttttaagtgc ttcttgctta agcaagtttc
     8701 atcccgaccc cctcagggtc gggatttttt tattgtgcat tcaacgattc acttcagaga
     8761 cgctgaagtc atgaatatcc agttcgaagg catccgccaa ttcacgccat gtatgatatt
     8821 cacgtccatc gacgctaacg tgttcagaat cgtgctggct gcgatgaact tcgctttcgc
     8881 taatttcatt gatcgccgcc agcagggcat cgacatcgac gctgacctca cgttttgcgg
     8941 tatcgctgta ctcttttgcg gttttcatca tctttcctcg caaccgttac tctgttgttg
     9001 ccgccagtcg ttaagtccgc ctggcatctc ttaagtatag accgtcgaga aaatcagcac
     9061 tcgcgcgcgc ctggcgctgc cagaaaaacg aaattgttct acactggcac aaagccacag
     9121 gaggaaaacg atgaaggtga atgatcgggt aacagtcaaa acggatggcg gtccgcgtcg
     9181 tcctggcgtg gtactggcag ttgaggagtt tagtgaaggc acaatgtacc tggtttcgct
     9241 ggaagactac ccgctcggca tctggttctt taatgaagca gggcatcagg acggtatctt
     9301 tgtggagaaa gcagagtaat cttattcagc ctgactggtg ggaaaccacc agtcagaatg
     9361 tgttagcgca tgttgacaaa aataccatta gtcacattat ccgtcagtcg gacgacatgg
     9421 tagataacct gtttattatg cgttttgatc ttacgtttaa tattaccttt atgcgatgaa
     9481 acggtcttgg ctttgatatt catttggtcg gagatttgaa tggttccctg acctgccatc
     9541 cacattcgca acatactcga ttcggttcgg ctcaatgata acgtcggcat atttaaaaac
     9601 gaggttatcg ttgtctcttt tttcagaata tcgccaagga tatcgtcgag agattccggt
     9661 ttaatcgatt tagaactgat caataaattt tttctgacca atagatattc atcaaaatga
     9721 acattggcaa ttgccataaa aacgataaat aacgtattgg gatgttgatt aatgatgagc
     9781 ttgatacgct gactgttaga agcatcgtgg atgaaacagt cctcattaat aaacaccact
     9841 gaagggcgct gtgaatcaca agctatggca aggtcatcaa cggtttcaat gtcgttgatt
     9901 tctctttttt taacccctct actcaacaga tacccggtta aacctagtcg ggtgtaacta
     9961 cataaatcca taataatcgt tgacatggca taccctcact caatgcgtaa cgataattcc
    10021 ccttacctga atatttcatc atgactaaac ggaacaacat gggtcaccta atgcgccact
    10081 ctcgcgattt ttcaggcgga cttactatcc cgtaaagtgt tgtataattt gcctggaatt
    10141 gtcttaaagt aaagtaaatg ttgcgatatg tgagtgagct taaaacaaat atttcgccgc
    10201 aggagtatcc tggaagatgt tcgtaggtat tatttttcgg ataatcctta ggataacatg
    10261 ataaacgtta cggaattata ttaatggcaa ttcactaata atatcagcca gcaaattaaa
    10321 catctcactg aataaatgtt cgcaaaaagg cgcaattaac ggcattaatg ccgccattaa
    10381 agagataccg acagtcagag ttaatggaaa tccaataaca aaaatggata attgcggggc
    10441 catacgatta agtaaaccta atgccagatt cagtgtcagc agcagagtaa tgagcggtaa
    10501 cgccagcatc agcccgttaa ggaaaatcaa actccctgct ttggtgagtg ccagaaacgc
    10561 attgctgttc aacggttcgc caccaatcgg cagggtgtga aaggtatcga ccagcagtga
    10621 aatcaaccat aaatgaccgt taaatgtcag gaacagcagt aacgccagca tatccatgat
    10681 acgcgctaaa acgggcatat tgagatggct ggccggatcg acaaacgtcg caaatgacag
    10741 ccccatttgc agaccgataa tttcgccagc ggttcgcaca gcggcaaagg caaattgcat
    10801 ggtaaaacca agcgcaatgc cgatcaggat ctgctgcacg gccagccaca gagcaaagaa
    10861 cgaaaaaaca ggaacatcgt tggcaggtaa tgatggggca atggcgaacg tgatcatcat
    10921 tgccagcccc agttttaccc gtttcggcac gctgcgttcg ctcagaattg gtgcggtaga
    10981 aatgagcgcc agcacgcgca gtaacggcca gaagtacagg cttaaccagg atagccattg
    11041 ttcgcttgtc acctgcaaca tagtacggct acccgatgat atacggcagg ttagtgaaca
    11101 aggtgcggac gtaatccagc aacagattga gcatccacgg tccggcaata ataatggcga
    11161 taaatacggc gatgattttc ggaataaacg acagcgtcat ttcgttaatc tgcgtggcag
    11221 cctgcaaaat actgatgata aggcccgtga ccaacgctac caacaatagc ggggcagcca
    11281 gtgccagcgc gactttcatc gcttcagtcc ccatcatcat gaccgattca ggtgtcattt
    11341 tgcctctcta gctgtaaaag ctctgcgcca gcgaaccgac cagcaattgc cagccatcca
    11401 ccagtacaaa cagcatcagt ttaaagggca gagcaatggt ggcgggggga accatcatca
    11461 tccccaatgc catcaacacg ctggctatca ccaggtcgat aatcaaaaaa gggatgaaaa
    11521 tcgtgaagcc tatctgaaat gcggttttca actcgctggt cacgtaggcc gggagcaaaa
    11581 tgcgcatcgg cacggcttca ggtccctgca acgggccggt attcgccagt ctggcaaaca
    11641 accctaaatc cgcctcacgg gtctgacgca gcataaactc acgcagcggc tgcgcccctt
    11701 tttccagcgc ctcctgcatt gatattttct cttcgctgaa tggctggtac gcatcgacat
    11761 aaattttgtc gatcaccggt gacataataa aaaaggtcaa aaacagtgcc agccccagca
    11821 atacctggtt aggtggcgcg gagggtgttc ccagcgcgtt acgcaataaa ccaaaaacaa
    11881 taatgatgcg ggtgaaactg gtcatcatca gtaaaattgc cggaataaac gtcaacgagg
    11941 tgatgaacac cagcgtttgc accgggagcg accagctttg tccaccgcca ggcagcggct
    12001 ggctggtgat acccggcagt tgcgcgaagg cgaggggcgt aatcagccag agaaggacag
    12061 gtgcgacaga caataaacga cgcatcagga tctcccgcta cgcttaagca aatttttcat
    12121 gaccgactga aaatcggtct gcggtatctc ttccgttggt gcagaagggg gaagtttatg
    12181 cagcagattg atttgccctg cggtaacgcc gagcaccagc cgtgcatctt ccacatcgac
    12241 caccacaacc cgttcacgcg cgcccagcga agcactggca ctaattttca gaccgttaac
    12301 gccagttcgt ttaggggcaa atcccagccg ttttactagc caggcagcag cgaggatcag
    12361 ggcaataatg gcgatgagtg cgccgctcac ctgcagcagt ggcgcagcag aaatcggcgc
    12421 ggaagattgc acagtagcgt ggttattcat cactaacggc tcaggcgacg cattcgctca
    12481 gacggggtaa taatatcggt gatccgcacg ccatatttat cagcgactac gacaacttcg
    12541 ccctgggcga ttaaataacc gttgatcaga atatccagtg gttcgcccgc cagaccgtcc
    12601 agcgccacga cggacccttg cgtcagacgc aacagctctt tgatggtcat ccgcgtacgg
    12661 cccagctcga cggtcagctt gaccggaata tccataatca ggtcgatatc ctgcaacgtt
    12721 ccgctgacat caccaccgcc aaattgctgg aacaccgtgt cggcagcgct tttgctactg
    12781 gttgattttt gttcgctcaa cgcttcagcc cacagatcgt ccattgcgcc gttgttgtca
    12841 tcggccggat tattcatgtc actcatttgg gctgttcctc gttcagagaa tttaaaatcg
    12901 ggttaatcaa atgttctatc cgtaacgcat actgaccgtt gagggtgccg tactgactgg
    12961 tcagcaccgg gacgccgtca acatgggcga tgatgcgatc gggtttttct atcggcagga
    13021 cgtcaccggg tttcagtttt aaaatctgcg acaggcgtag cgagatatcg gcaaagttgg
    13081 cgaccagctc cagctgtgaa tgctgcacct ggcgcaccag gttatcgcgc cagttctgat
    13141 cttcattacg cgagttttcc agcggcgggt taaccaacaa ttcccgtagc ggctcgatca
    13201 tgctgaaagg caggcagata ttaaattcgc cggtcaggtt gccaatctcc acatggaacg
    13261 gcgtgttaac cacaatgtcg ttcggcgagg tggtgatatt ggtaaatttc acctgcattt
    13321 ccgaacgcac gtactcaact tccagcggat taatcgcctt ccatgcgtcg ctatagcctt
    13381 caagcgccag tttcaacatg cggttgatga cgcgctgttc ggtatgggta aactcgcgac
    13441 cttccacttt ggtcgggaag cgtccatcgc cgccaaacag gttatccacg gcgataaaca
    13501 ccagactcgg cgagaacacc accagccctg tgccgcgcag cggtttcaga tggataaggt
    13561 tcaggttggt cggcaccggc aggttgcggg caaattcatg gtacggctga atgcggatgg
    13621 ccccgacggt aatatccggg ctacggcgca gcaggttgaa cagccccata cgaaaatggc
    13681 gagcaaagcg ctcattaatg atttccagcg cctgcaaacg ttcgcgcaca acccgtcgtt
    13741 gggtattcgg atcgtacgga cgaatgtcac tttcgccgct aacactggct gtcggttcgt
    13801 ctttgacttc gctgtcacca ttcaacagcg catcaatttc agcttgagaa agaatactat
    13861 cgcccatgtc gttatcgcag aataaaagcg gtatacagca cgtcggtgac atcctgtttc
    13921 ggttgcccgg caacaagcgg ggtggaaagc gtggttttaa tctcggcaat caggtttttc
    13981 ttgccttctt ctgtcgccag tacggcagca tcctgacgcg aaaacagtaa cagcaagcga
    14041 ctacggactt ccggcaaata ctcactcagc cgcgagcggg tagcttcatc tttcaggcgc
    14101 agggttatgc cgatataaag cacgcgatcc gcatcgccca gattgaccgt aaaggtatcc
    14161 agcgcataga agaccggtga gggcacgacg cgttgctgcg ctttgtcgtc agcggcaacc
    14221 tgatgcgaat gccagtagct gtaacctgcg ctggcacagg ccgcgagggt aatgaatacc
    14281 agaatcggga tccaaagcga tcgcttgctt ttcttgctta tcgcgtaatc agtcatgtgt
    14341 tgcggtcttc ctgtgtcgct actgcttatc ggctaattat cctgcgtctt gagcgcaaca
    14401 aagcggggaa aagacgcgga ttacggtgct acctctgacg ttaggcgaaa atatcaacgc
    14461 cgctgttgcc tgttacacgc ccttgtaaag agacgggaac cggaagcgta tcgtcgtctt
    14521 cccccgccag aggttcatgg tttactgtgc gttggctttg ctgttgttgg gaagcggcct
    14581 gctgctgacc actaaagctt tcgccactga tgttgctttg ccctaactga atgccacttt
    14641 cggccagctg cgtacgcagt accggcagcg ctgcttccag ggcggcgcgt acatgctgat
    14701 ggggtgaaac catctggatt tgcgcctggt tatcatccac tttgagggag atttgcactt
    14761 cacctaaatc ctgcgggtgc agacgcaact ctgcactttg ttgcccctgg cgggtgaaca
    14821 gcgaaatatg ctggcttaat gattgttgcc attcgtgaga acccagcggt gcactcaaaa
    14881 caggcgcggc gacggtgggc agtggctgtg tctggtgtgg agcgattagc gggctggcgg
    14941 cagcggtcac cggtgaaggt gtgctgatga cttccgcttt actctgggct tctgctacca
    15001 gcggtgttaa tggctgagct ggcgtgccgg gggcgtcatc aggctgtgct gttgtgagtt
    15061 gcgtagaagt cagttttgtg aagagcgttg gtttctctgt cggtaacacg gttgacggcg
    15121 catcagtcac tttgggcgta ttgtcaaaac ccggcaacat cgcaaaaagg gcactcaggc
    15181 ttgcggtgac gtcttcattc agatcatccg ctttttcgtc ttttgccgta ttgttgccag
    15241 ccaccgcagc caacgtcatc gtttgagcgg tggttaatgg tgttgatgtg gattgttcgt
    15301 cgttgatgac aggcagtgtt tcatccacgg ggatcagtaa atccgcttgt tgcgcgtcgg
    15361 aaacaatatc gctgaccagc ggctcgcctt ttgtcgtggg cttatctgtt gccaccagca
    15421 actggggagt cgctttgtcg gttgttgcct cgcctgttaa tgcttcgctc aacaacgcga
    15481 gaaaatcttg tgcagcatcg ctggctttgc cgccagacaa tgtggtgtta tcaacgtcag
    15541 cggtaatcaa gggcgctatg cgaatcattc aggtttcctc gtggcggcgc gctgggcgaa
    15601 ctcatccatc tttttctgat cgaggcggtt ttctgcaagc agtgccgccg tggattgccg
    15661 ttcctgcagt gtctgccagg cctgcaaacg ttgtttcttt tctcgccagc tgttcagggc
    15721 aatgtcaact ttctgcgtcc actgattaag ttgctggcga tgctgagtaa tggctttttc
    15781 cagcgtctgg ataaactgct gatagttgat ccagcggttg ctggtcatcc cggcactcat
    15841 atcgctgttg aggttattgc gatattcatt ctgataatcg atcagcattt tgagctgttc
    15901 ttccgcctgc tgacatccgc gacgcatttc acccagcagg cgcgcggcat cctctacctc
    15961 tttttctgcc agatctttca gggtcgccag cgcgccatgt tctgtcatct gccgttatct
    16021 cctgggttat gacactgtcg ggaaaatacg ctccagcccc tggagagacg cttcccagtc
    16081 cgcgcgttca aaaatgcctt gttgcaaata gccctccagc tgcggccaca gggcgatggc
    16141 tttatcgagc atcggatcgc tgcctttggc atacgcgccg acgctaacca gatcgcggtt
    16201 acgctgaaaa ctcgacaaca gctgtttgaa ggtgcgcact cgcgcgtaat gttgctcact
    16261 gatcaacgcc gtcattgcgc ggctgatcga cgcttcaata tcaatcgccg gatagtgccc
    16321 ggcttccgcc agtcggcgag acagcacaat atgaccgtcg aggatggccc gcgcggagtc
    16381 ggcaatgggg tcctgctggt catcgccttc ggtgagcacg gtataaaacg cggtaatcga
    16441 gccgccgccg ctaatgccat ttccggcacg ctcgaccagt gccggtaatt tggcaaacac
    16501 cgacggtgga taacctttgg tggcgggtgg ttcgccaatc gccagcgcaa tttcacgctg
    16561 ggccatcgcg tagcgggtga gggagtccat aatcagcaac acatgctgac cacgatcgcg
    16621 aaaatcttcg gcaatgcgcg tggcataggc ggcaccttgc attcgcagga gcggagacac
    16681 atccgccgga gcggcaatca ccactgatcg tgcacgccct tcggcaccga ggatattctc
    16741 aataaaatct tttacttcgc gcccacgttc accaatcaaa cccacgacaa tgacatcggc
    16801 gcgggtgtaa cgggccatca tccccagcag cacactttta ccgacaccag aaccagcaaa
    16861 cagacccata cgctgcccgc gcccaacggt aagcagggca ttgattgggc gcacgccggt
    16921 gtccagcaca tgttcaatcg gtgtacgttg caatgggtta aatggtgggg taatcagcgc
    16981 accggtttcc gtcgtatcgg gggagggcag gccatcgagc ggtttaccgc tgccgtccag
    17041 aacgcgacct aataacgccg gaccgagcgg caactgtttg ccgctttgta gcccttcagg
    17101 cgaaatgttt ttggcataaa cacgcgcgcc gggcaggaca ccttccactt cctccagcgg
    17161 cattaaaaac agccgttgac cgttaaagcc aacgacttcg ctttctactt cgtgcgtttc
    17221 gctgccgttc tggcgctcaa tgacacaggt tgcgccgagc ggcaattgta atccggtggc
    17281 ttccagcacc agcccggtag cgcgggttaa tcgcccgtag cgacgtaccg caggcaactg
    17341 cgccattttg gcttcaaagt tatccagcgt ggttagccag cgagtcaggc gcgtggtcat
    17401 tacaccactc ctggtgctgc cagacggcag agttcttgcc agcgagtggc gacactggcg
    17461 tcgagatcgc cttcatcggc ggagacttta cagccgccag gatggagggt gggatcgccc
    17521 cgcaagcgcc agccatgcaa acttaaggta gcgccgagca tatcatccac acgttgcaga
    17581 tcatccgggt gcacgcgcag ctgtggttta ccgctgaata acggttcttg ctgcaacaac
    17641 tgttggatct gtttgatcag tgccgagtta tccactgttg gcgtctgacc gatgacctga
    17701 cgtgccgcct ctagcgccat ctgcatcagg cgcgacgcta tcacactatc aagtgcatca
    17761 agggtagttt gaaattcgct gaccagttgc tgcatccggg catgaattgg cgcttgttga
    17821 gacttcgcct ctgccagacc ttgctccagc ccctgagcca gtccttcctg atagccctgc
    17881 ttatgacctt gctggcgacc ttcggcaatc cccgcctgat aaccttgctc gtgggcctgc
    17941 atttgcagtt gcgccagttg ctgctcaagg ctgggttcgg tctcttcaat gatggtttct
    18001 tccggctcaa ctattggcac aaactctgcc tgtggtggcg cgagatcgtc cggcgtccag
    18061 gttttccagg gcagattatc agacataggt atcctcgccg ctgccaatta ccatctcgcc
    18121 agtttcggca aggcggcgca caatcagcag aatcgctttc tgttcgtttt ccacctgcga
    18181 cagacgcacc ggaccacggt tggcgagatc gtcgcgcaga atatcggcgg cacgctgcga
    18241 catattgcgc aggaatttct cgcgcagtgg ctgctcggcc cctttcagcg cgatcaacag
    18301 cgattcggaa tccacttcct gcaacagacg ctgaatgctg cgatcgtcga catccaccag
    18361 attctcgaac aggaacatct cgtcgatgat tttctgcgcc agttcgccgt cgaattcacg
    18421 cacggcggta ataacggctt cttcctgctg agttttcatc aggttgataa tttcggctgc
    18481 cgttctcacg ccgcccattt tgctgcgctt gagattctga ccgtcgagca agccattcag
    18541 tacttcggtc agctctgcca gcgcggctgg ctgcacgccg ccaaaggtgg cgatacgcaa
    18601 catcacgtcg tggcgcagac gttcatcgaa caacgccaga atatcggcgg cctgggcgcg
    18661 cttcagatgc accagaatgg tggcgataat ttgcggatgc tcatcgcgaa tcagatcggc
    18721 ggcgctctgt ggctccataa agttgagcgt ttcaataccg ctggcggtat cgcgagtttc
    18781 gagaatatct tccagcaggc tggcggcacg ttcttcaccc agagctttga ccaataccga
    18841 acgcagataa tcgttggcgt tgatattcag tgcggcaaac tgttcagctt cttgctcaaa
    18901 ctccgccagc acatcggtta gctgcttgtt ggagatctgc gtgacgttcg ccattgcagc
    18961 gctcagggtt tgcacctcgc gctgggagag gtgcttgaac acctctgccg cccggtcttc
    19021 gccaatggtc atcagcagga tgacgctttt atcggtgcct gtcaggttac tcatgatcgt
    19081 tatttatcca ctggcgaatg accagcgcca ccacgcgcgg atcgttatca gacatttcac
    19141 ggatacgctg gctcatgact tctgccccca gacgttggtt agcgcgccgt tgttggagtt
    19201 gttcgtcttt gctcaggcgg acttccaccg catcttccac ttcctcgcgg gcctgcgcct
    19261 gttgctgtac agctttcatc gcctcagcgc gacgtgttaa ctgcgggcgt accgctttac
    19321 gccacagcag ccacgccacc agcagtacca gcaaccagcg accggcagcc agtaactgat
    19381 cgataaacgc ttgctgttgc cagaacggca gttctccgcc gctttcctca ctactattga
    19441 acggcgagtt aacgacattg agcgagtcac cgcgtttttc agaaaagccc atcgcctcgc
    19501 gggtcagatc ttcaatttgc ttcatctgtt cgttgctgag aggcaacggt ttaccgtctg
    19561 gcaaggtttt gtaattcacc acaaccgcga ctgacagacg ttgcacatcg cccacgttca
    19621 ttttggtatg acgaatggtg cgatcgactt cgtagttact ggtttcattc cgctgtgtgc
    19681 tacgcggccc gctattgctg gtggtgctcg cctgctgctg gcggttattt tgatttgccg
    19741 gaggcgtgct gattggcgcg ttattcgcag gtgccggttg attcgacaac gcccccggta
    19801 cgccgcccgg ataaccggaa ccgctttgct cgctctcatt aagctggcgt gaacgaagcg
    19861 ccgcctgaga ttcatcaccg ttagggcgat actgttcttc cgtttgttct ttactggcga
    19921 agtccagctg cgccgtaacc tgggcgtgaa tattaccgtt accaacaata ggcgacagga
    19981 ttgcttcaat acgccgctga atacgtcctt cgacatcgct ggcatatttc aattgagcgt
    20041 cattaagatc gcgcccgctg gtattggact gggttaacag atgtccgccc tgatccacca
    20101 gcgtgacgtt tcccggcggc agaccagcaa cggcgctgga aaccagatgc acaatggcgc
    20161 taatttgccc ttcatcgagt gcgcggccgg gtaacagatt taccgtcacc gatgcagaag
    20221 gggatttttg ttcacggacg aataaagacg gtttcggcat tgccagatgt acgcgcgccc
    20281 cttttaccgg gccgatagtt tcgatggtac gagaaagctc gccttccagc gcccgctgat
    20341 aattcacctg ttcgctgaac tggctgatac caaacttttc ctgatcaagc agttcgaaac
    20401 cgaccgcgcc gccttttggc aaaccttgtt gtgccaggcg cagacgcagt tcgtgaactt
    20461 tatctgccgg aacttcaata gcgccgctgg cttcgctgaa gcggtaagga atattcattt
    20521 gcgtcagttg gctgacaatt gcgccaccat cctgatcgga aagattgctg aataatgtgc
    20581 ggtagtcggg ggctttcgcc cacaggatca gtgcgaccat gaccgccact gccgcggaac
    20641 cggcaacaat caatggaatt ttcggattcg cacgcaggcg attaagccac tcaagagatt
    20701 ttgtctgggc tgcagtcgca ttcatcgcgc acctcgtggc tgattatgga cggcgttatt
    20761 ggcaaagtgg aacaagactc actcccgggt cagcaaactc gaaaaattga cgctcccatt
    20821 atttgtgctt tcgttttttt ctatccgcca ataaacccgt ttttttgttg ctatttagcg
    20881 cctttgtctt attgacttac tggtaggctt tgctaccaga aattatccag gagatgaaaa
    20941 tgtcagcgat acaggggatt gaaggggtta tcagccagtt acaggctacg gcgatgagtg
    21001 cgcgtgcgca ggaatcactg ccgcaaccga ccattagttt tgccgggcag ctgcacgccg
    21061 cgctcgatcg cataagcgat acacaaacag ccgcccgcac gcaggcagaa aaattcactc
    21121 tcggtgaacc cggcgtggcg ttaaacgatg tgatgaccga tatgcaaaaa gcctcagttt
    21181 ctatgcaaat ggggattcag gtgcgtaata agctggtggc ggcgtatcag gaagtgatga
    21241 gcatgcaggt gtaggttttg taacctgttg ttaattacat ccgatctcac cgcgtggggc
    21301 atggatgggg caaactcact caatttctgg tttaaaatgg aaacctgatt ctgaaagttt
    21361 tctgccatcc acgatccgta tacgcgataa accttatgat catcagaatg tctcatttga
    21421 cacacatcac cacgtcggat gttgttacga ccacagacgc aacagtaatc tctggataat
    21481 ttaatcttcg ttcttatttt tattaatata ctgcgttata aaaatgagcg atttttgata
    21541 gtggtaattt tattgtgttt tagaatgctc cctgggcaa
//
LOCUS       DAZGBC010000081        21444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGBC010000081 DAZGBC010000000
VERSION     DAZGBC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.081"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(108..1016)
                     /gene="melR"
                     /locus_tag="ACHK1J_002900"
     CDS             complement(108..1016)
                     /gene="melR"
                     /locus_tag="ACHK1J_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO7912143.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1299..2654
                     /gene="melA"
                     /locus_tag="ACHK1J_002901"
     CDS             1299..2654
                     /gene="melA"
                     /locus_tag="ACHK1J_002901"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO7912144.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2757..4178
                     /gene="melB"
                     /locus_tag="ACHK1J_002902"
     CDS             2757..4178
                     /gene="melB"
                     /locus_tag="ACHK1J_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO7912145.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4317..4946)
                     /locus_tag="ACHK1J_002903"
     CDS             complement(4317..4946)
                     /locus_tag="ACHK1J_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO7912146.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5069..6715)
                     /gene="fumB"
                     /locus_tag="ACHK1J_002904"
     CDS             complement(5069..6715)
                     /gene="fumB"
                     /locus_tag="ACHK1J_002904"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7912147.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6793..8133)
                     /gene="dcuB"
                     /locus_tag="ACHK1J_002905"
     CDS             complement(6793..8133)
                     /gene="dcuB"
                     /locus_tag="ACHK1J_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO7912148.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8704..9423)
                     /gene="dcuR"
                     /locus_tag="ACHK1J_002906"
     CDS             complement(8704..9423)
                     /gene="dcuR"
                     /locus_tag="ACHK1J_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO7912149.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9420..11051)
                     /locus_tag="ACHK1J_002907"
     CDS             complement(9420..11051)
                     /locus_tag="ACHK1J_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO7912150.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAMARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11232..11462
                     /gene="yjdI"
                     /locus_tag="ACHK1J_002908"
     CDS             11232..11462
                     /gene="yjdI"
                     /locus_tag="ACHK1J_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO7912151.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKCRHK"
     gene            11474..11746
                     /locus_tag="ACHK1J_002909"
     CDS             11474..11746
                     /locus_tag="ACHK1J_002909"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7912152.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11973..12269
                     /gene="ghoS"
                     /locus_tag="ACHK1J_002910"
     CDS             11973..12269
                     /gene="ghoS"
                     /locus_tag="ACHK1J_002910"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7912153.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12297..12470
                     /gene="ghoT"
                     /locus_tag="ACHK1J_002911"
     CDS             12297..12470
                     /gene="ghoT"
                     /locus_tag="ACHK1J_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO7912154.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12589..14106)
                     /gene="lysS"
                     /locus_tag="ACHK1J_002912"
     CDS             complement(12589..14106)
                     /gene="lysS"
                     /locus_tag="ACHK1J_002912"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7912155.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGDRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14343..15800)
                     /gene="dtpC"
                     /locus_tag="ACHK1J_002913"
     CDS             complement(14343..15800)
                     /gene="dtpC"
                     /locus_tag="ACHK1J_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO7912156.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(15859..18006)
                     /gene="cadA"
                     /locus_tag="ACHK1J_002914"
     CDS             complement(15859..18006)
                     /gene="cadA"
                     /locus_tag="ACHK1J_002914"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO7912157.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18086..19420)
                     /gene="cadB"
                     /locus_tag="ACHK1J_002915"
     CDS             complement(18086..19420)
                     /gene="cadB"
                     /locus_tag="ACHK1J_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO7912158.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(19785..21323)
                     /gene="cadC"
                     /locus_tag="ACHK1J_002916"
     CDS             complement(19785..21323)
                     /gene="cadC"
                     /locus_tag="ACHK1J_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO7912159.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         5569 a         5048 c         5240 g         5587 t
ORIGIN      
        1 tgaaatattt aattttataa acaatacgtt atgtgattat tttaaattat tttcactgct
       61 tattatcgac ggctaaacta tttttttggc tgatactgat atcgtcttta gccgggaaac
      121 gtctggcggc gctgttggct aagtttgcgg tattgttgcg gcgacatgcc gacatatttg
      181 ccgaacgtgc tgtaaaaacg actacttgaa cgaaagcctg ccgtcagggc aatatcgaga
      241 atacttttat cggtatcgct cagtaacgcg cgaacgtggt tgatgcgcat cgcggtaatg
      301 tactgtttca tcgtcaattg catgacccgc tggaatatcc ccattgcata gttggcgtta
      361 agtttgacgt gctcagccac atcgttgatg gtcagcgcct gatcatagtt ttcggcaata
      421 aagcccagca tctggctaac ataaaattgc gcatggcgcg agacgctgtt tttgtgtgtg
      481 cgcgaggttt tattgaccag aatcggttcc cagccagaga ggctaaatcg cttgagcatc
      541 aggccaattt catcaatggc gagctggcga atttgctcgt tcggactgtt taattcctgc
      601 tgccagcggc gcacttcaaa cgggctaagt tgctgtgtgg ccagtgattt gatcaccatg
      661 ccgtgagtga cgtggttaat caggtcttta tccagcggcc aggagagaaa cagatgcatc
      721 ggcagattaa aaatcgccat gctctgacag gttccggtat ctgttagttg gtgcggtgta
      781 caggcccaga acagcgtgat atgaccctga ttgatattca ctttttcatt gttgatcagg
      841 tattccacat cgccatcgaa aggcacattc acttcgacct gaccatgcca gtggctggtg
      901 ggcatgatat gcggtgcgcg aaactcaatc tccatccgct ggtattccga atacagcgac
      961 agcgggctgc gggtctgttt ttcgtcgctg ctgcacataa acgtatctgt attcatggat
     1021 ggctctcttt cctggaatat cagaattatg gcaggagtga gggaggatga ctgcgagtgg
     1081 gagcacggtt ttcaccctct tcccagagag gcgaggggag cgtccgagca tcatgaggcc
     1141 gaaaactctg cttttcaggt aacttattac cataaactca gatttactgc tgcttcacgc
     1201 aggatctgag tttatgggaa tgctcaacct ggaagccgga ggttttctgc agattcgcct
     1261 gccatgatga agttattcaa gcaagccagg agatctgcat gatgactgca cccaaaatta
     1321 catttatcgg cgctggttcg acgattttcg ttaaaaatat tcttggtgat gtgttccatc
     1381 gcgaggcgct gaaaacggcg catattgccc tgatggacat tgatcccacc cgcctggaag
     1441 agtcgcatat tgtggtgcgt aagctgatgg attcagcagg ggccagcggc aaaatcacct
     1501 gccacaccca acagaaagaa gccttacagg atgccgattt tgtcgtggtg gcatttcaga
     1561 ttggcggtta tgaaccttgc acggtgactg atttcgaggt ctgtaagcgg catggtctgg
     1621 aacaaaccat tgccgatacg ttggggccgg gcggtattat gcgcgcgcta cgtaccattc
     1681 cgcatctgtg gcaaatttgc gaggacatga cggaagtctg ccccgatgcc accatgctca
     1741 actatgttaa cccaatggcg atgaatacct gggcgatgta tgcccgctat ccgcatatca
     1801 aacaggtcgg gctgtgccat tcggtgcagg gaacggcgga agagctggcg cgtgacctca
     1861 atatcgaccc agctacgctg cgttaccgtt gcgcaggtat caaccatatg gcgttttacc
     1921 tggagctgga gcgcaaaacc gccgacggca gttatgtgaa tctctacccg gaactgctgg
     1981 cggcttatga agcagggcag gcaccgaagc cgaatattca tggcaatact cgctgccaga
     2041 atattgtgcg ctacgaaatg ttcaaaaagc tgggctactt cgtcacggaa tcgtcagaac
     2101 attttgctga gtacacaccg tggtttatta agccaggtcg tgaggatttg attgagcgtt
     2161 ataaagtacc gctggatgag tacccgaaac gctgcgtcga gcagctggcg aactggcata
     2221 aagagctgga ggagtataaa aacgcctccc ggattgatat taaaccgtca cgggaatatg
     2281 ccagcacaat catgaacgct atctggactg gcgagccgag tgtgatttac ggcaacgtcc
     2341 gtaacgatgg tttgattgat aacctgccac aaggatgttg cgtggaagta gcctgtctgg
     2401 ttgatgctaa tggcattcag ccgaccaaag tcggtacgct accttcgcat ctggccgccc
     2461 tgatgcaaac caacatcaac gtacagacgc tgctgaccga agctattctt acggaaaatc
     2521 gcgaccgtgt ttaccacgcc gcgatgatgg acccgcatac tgctgccgtg ctgggcattg
     2581 atgaaatata tgctcttgtt gacgacctga ttgccgccca cggcgactgg ctgccaggct
     2641 ggttgcaccg ttaaaacgcg actaaacgct actgcgccgg gggatttatt ccggcgcaca
     2701 cctctgacga taccaataac agaaggcggg cgttggtaac agcgacccga taccctatga
     2761 gcatttcaat gactacaaaa ctcagttatg gatttggagc gttcgggaag gattttgcga
     2821 tcggcattgt gtatatgtac ctcatgtatt actacaccga tgtcgttggg ctgtctgtgg
     2881 gtttggtcgg tactttgttt ctggtggcga ggatctggga tgctattaac gatccgatta
     2941 tgggatggat tgtaaatgct acgcgatcgc gatggggtaa gttcaaaccc tggatcctga
     3001 tcggtacgtt ggcaaactct gtaatcttat ttctcctctt tagtgcgcat ctgtttgaag
     3061 gtactactca gattgtcttt gtttgcgtga cctacatcct ctggggcatg acttacacca
     3121 ttatggatat tcccttctgg tcgctggttc caaccatcac gctcgataaa cgtgagcgcg
     3181 aacaactggt tccttatccg cgtttttttg ccagtctggc aggctttgtt acggcaggtg
     3241 tgacgctacc atttgttaat tatgtcggcg gtggcgatcg gggatttggc tttcagatgt
     3301 tcactctggt actaatcgcc ttttttattg tttcaaccat catcactctg cgcaatgtgc
     3361 atgaagtctt ttcgtcagac aatcaaccgt ctgctgaagg aagccatctg acacttaaag
     3421 ccatcgttgc gctaatttat aaaaacgatc agctttcatg cctcttgggt atggctcttg
     3481 cttataatgt agccagcaac attattaccg gatttgctat ctattatttc tcatatgtta
     3541 tcggtgatgc ggatttgttc ccctattatc tgtcgtatgc gggagctgct aacctggtga
     3601 cgttagtatt cttcccacgc ttagttaaat cattatcccg acgcatttta tgggccggag
     3661 catctattct tccggtgtta agctgtggtg ttctcctgtt aatggcatta atgagctatc
     3721 acaacgtcgt cctcattgtg attgcgggta ttttgctgaa tgtgggaacg gcgcttttct
     3781 gggtattaca ggtcatcatg gtggcagata ccgttgatta cggtgaatat aaactgcacg
     3841 tacgctgtga aagtatcgct tactccgtgc agactatggt ggtgaagggc ggttcagcct
     3901 ttgcggcttt tttcattgcg gttgtgttag ggatgattgg ctatgtaccg aatgttgaac
     3961 agtctacgca agccctatta ggtatgcagt ttattatgat tgctctacca actctgtttt
     4021 tcatggtaac gctgattctc tacttccgtt tctatcgcct caatggcgac acgctgcgca
     4081 ggatccagat tcatctgctg gataaatatc gcaaagtacc gcccgagcct gttcatgctg
     4141 atattccggt cggtgcagtg agtgatgtga aagcctgacg tggacaatag ctaacgaaaa
     4201 tggcctgatg cgatatgttt atcaggccac gtcttaatgt aatgctttgg atttgcaaat
     4261 tttgtagacc ggataaggaa ttcacgccgc atccggcatc aacaaagcgc acgttgttat
     4321 ccggttatca agccaaagcg ccgtagctgg cggcaatgaa agcgagcgag gaatatcacc
     4381 gtcgttaatg cgccaagcag cgcgcagaac atatcggatt gcgtgtccca ctggtcgccc
     4441 tgggtgccga gaaaatcatc cgctccctgg cccattgcca gcgccgccca ccactcgatt
     4501 aattcataca tcgcgcttat cgccagcgcc acgcagcaga ccaggaacgc caccatttta
     4561 cgtccgcgca cgtacatccc gcgcacgaga atttctcgtg ccaccagtgc aggcaccagc
     4621 ccctggaaaa aatgccccag cttgtcatac ggattacggc tcaaccccag ccattcctgc
     4681 acctcaaaac cgatggggac tttcgcgtag gtgtattgcc cgccgaccat caggatgatg
     4741 gcgtgaaaga aaatgagcgt atagagcagc ggcgttaacg gataacgttt agcggtggca
     4801 agcagtagcg gcacgacaat aatcaccggt gtcacttcca tcagccaggt gagtttgtca
     4861 tgggccgaaa tgccggtata aatcaggatt aacgttagcg ccagtgcgcc ggtgttaaga
     4921 attaacggct tgagtgtgcg ggtcatggtg agttcgcaag tcagggaaaa tactgactat
     4981 tcaccagcaa gcgtgaaatt acaatcgcaa aagaggtggc ccagggggat cacctggcag
     5041 catgctgcca ggcgctgggc cgaagaggtt acttagtgca gttcgcgcac tgtttgttga
     5101 cgatctgctg gaagaagtcg ttacctttgt catcgaccag gataaacgcc gggaaatctt
     5161 ctacttcgat tttccagata gcttccatac ccagctccgg ataagcgacg cattccagat
     5221 gcttgatgct ctgctgcgcc agtaccgccg ccggaccgcc gatgctaccg aggtagaagc
     5281 cgccgtgttt atgacacgcg tcggtaacct gctgactgcg gttacctttc gccagcatga
     5341 tcatgctgcc gccgtgggat tgcagcagat ccacgtagga gtccatacgg cctgcggtgg
     5401 ttgggccaag tgaacctgat ggataaccgg caggggtttt cgccggaccc gcgtagtaga
     5461 tcgggtgatc tttgatgtac tgcggaagtt ctttaccggc gtcaatcagc tctttcagct
     5521 tggcgtgtgc aatatctcgg cccacgataa tggtgccggt gagcgacaaa cgagtggata
     5581 ccgggtattg cgaaagctgg gcgaggatct ctttcatcgg gcggttaagg tcaactttca
     5641 ccgcttcgcc ttcaccggcc tggcgcagtt cttgtggaat gtactggcct gggttgtgtt
     5701 ccagtttttc gatccagata ccttcgcggt tgattttcgc tttaatgtta cggtcagcgg
     5761 agcaggagac gcccatgccg accgggcagg atgcgccgtg acgtggcaga cggataacgc
     5821 gaatgtcgtg cgcgaagtat ttaccgccaa actgcgcgcc aagaccgagt ttctgggcct
     5881 cttccagcag ttcctgttcc agctggacat cgcggaacgc ctgaccatgt tcgttccctt
     5941 ccgtcggcag ttcatcgtaa tagtgagcgc ttgctaactt gacggttttc aggttggttt
     6001 ccgcagacgt accgccaatc acaaacgcga tatggtacgg cgggcaggct gcagtaccga
     6061 gggtacgcat tttctcgacg aggaagtttt tcagtttgcc gggagtcagc agggctttgg
     6121 tttcctggta gagatacgtt ttgttggcag agccgccgcc tttcgcaacg caaaggaatt
     6181 tgtactcatc gccatctacc gcgtacaggt cgatttgcgc aggcaggtta gtgccggtgt
     6241 tgacctcttt gtacatgtcc agcgccgcat tctgtgaata gcgcaggtta tcttcgatat
     6301 aggtgttata gacgcctttc gacagcgctt cttcatcacc gccgccggtc cacacgcgct
     6361 ggcctttttt accgacgatg atcgcggtgc cggtatcctg gcaggtcggc agcacgcctt
     6421 tggcggcgat ttcggagttt cttaagaatt gcagcgccac gtacttgtcg ttttcgctgg
     6481 cttctggatc gtgaagaata gccgcaacct gtttctggtg tgccgggcgg agcataaaag
     6541 aagcgtcgtg aaaggcttgc tgcgccagca gggtcagggc ttctggttcc actttcagga
     6601 tggtttcgcc gtcgaagtcg gcaacgctaa cgtaatcgga agtgagtaga tagtattcgg
     6661 tattgtcttt ccccatcggg aaaggtgcct ggtagataaa gggtttgttt gacatagctt
     6721 ccagcctgta actctgtatt tgttattcga aaatggcgtg ccgcgtagtc acgggcggca
     6781 cgcaaagtgc atttataaga acccgtacat cgcggcgaag atccagccga agacgcacga
     6841 tacgctcaca ccaatcaacc ccggcagaat aaagctgtgg ttgatgacga agcgaccgat
     6901 gtgggtggtg ccggaacggt caaactgaat cgctgccaga tcgctcggat aagtcggcag
     6961 gatgtaataa ccgtagcaag ccggtgctga agccacgatg tatgccggat caacgccgat
     7021 cgccagcgcg accggaacaa tcgccgccag cgccgcagcc tgagagttta caaacttgga
     7081 aaccagcagc agaacaatgg cataggccca cggatactct ttcaccattt cacccagtac
     7141 gccctgaatt tcagacatat gcgcaccgaa catggtttct gccatccatg cgataccgta
     7201 caccgccacg atggcgatca taccggaacg gaagacttcg ttttttgaga tagacgcggg
     7261 attggttttg gtcaggataa taatcagcgc cccggtcagc agcataaaca tctgaataac
     7321 cagtaccatc gacagcggtt tgccgccgaa ggatggacgc aggtccgaat cagcaccaag
     7381 aagtgcgact acagcgattg caccgaggaa aatccacatt gccagccagt tgcttttcgg
     7441 cagtttttta tcgagcagcg tcgcggtatc accgtaaaca tactcacggt tttccggtac
     7501 ggagatgaat ttctggaact cttcgtcttt atccagatct ttaccgcgga accagctgaa
     7561 gataccgatc gccaggatac cgattaacgt cgatggaatg gtgattgcca gcaggtcgag
     7621 gaactcaaga tggcgaccat caaaggtgac attacccagc attgcaacca gagacacgac
     7681 cgcaaccgac accggactgg cgataatccc catctgtgca ccgatagaac ttgccgccat
     7741 cggacgttcc ggacggatgt tgttcttaat ggcgacgtcg tagatgatcg gcagaatggt
     7801 gtaaaccaca tgacccgtac cgcaaagaat ggtcagtgta caggtcacaa acggcgcgac
     7861 aattgagaca tatttcgggt tgcggcgcag caacttctcg gcaatttgca gcatgacatc
     7921 aagaccgccc gaagcttgca aggtcgccga tgccgccacc accgcaatga taaccagcat
     7981 gacatcaact ggtggtttac ctggctgaag gtggaagacg aagaccagaa tgaccagacc
     8041 gataccgcct aataaaccca gcgcgatacc accctttctg gcaccataaa acagacatat
     8101 cagtattatg ataagttgga tagtaaataa catgtgtgaa ccctcgcgat aatcctattt
     8161 aaatttttgc tgaatagatc acagtcacgt tctgttttgt atgaactgtt ttcagagcct
     8221 gactaaatat ccgtttggtc gttaccggct ttagcaaata cctcacagtg aatattggct
     8281 gattaaagca gcggcaaatt tatgcactgt gataagcggc tttttcagag agaaagactc
     8341 tctggcgatg ggtttatagt ttgtactgcg cagatgttaa tgttttgttt gctgttatct
     8401 tattgatatt tatcggtcta ttttttgttc tcagggcaga tgtcattagg tttatagatt
     8461 aatctgatct acccatttgt gggtaaaaat acacataatg cgggtgacat aatagttaat
     8521 taacttttgt tagcgttttg aaattaaaaa caccgttcac ctgaagagat attaattttt
     8581 agcgatgatg gagggataat tatatttgat ctggcacaag ttttactgat gaaggatgta
     8641 acttgtgcca ggggtatttt gcattacggt aataattatt acttacagat aaccagcgat
     8701 ccgttattgg caatattgtt tcagtagtga gtagtgttct gcctgaatac ggtaacggta
     8761 aactggacgc cccgtgacgc cataatggat actggtgaac aagatgtggc agttgaccag
     8821 ccagatgagg tatttacggc aggaaacacg cgagatgtta acctcgttag ccagctcgtc
     8881 ggttgaaaac tcatagtcct gatgcgcgtc aatccactgg cacagcgtgc gtaacgtctg
     8941 cggcgttaag ccttttggca agcgacgagg atcctgttcg ttggagctgc tgccgtggat
     9001 tagctgatca agctcggcct ggtcataata ctgatgtttt tccagcgcca ttttcttttg
     9061 ccgccagccg gtgagcgcct cttcaaagcg ggaagcctgg aagggtttga tcaggtaatc
     9121 cacgacaccg taatgcagcg aatctttaat ggttgccgca tcggctgcgg aggagatgac
     9181 aatcacatca cttttgcaac gcgcgttatg caggacaggc agtaaatcga gcccgttctc
     9241 tttttgcata tagatatcga gcaatatcag gtcgataggc gtatcgctat tgaagataat
     9301 ctctttggct ttctccagcg tcgaggctgt tccacagcat tgaaagcctg ggatttgtgc
     9361 tacgtatcgg cgattcagct ccgcgaccat tgcgtcgtca tcgataatta atacattgat
     9421 catctgttcg acctctcccc gtcccagggt atctggacaa aaaattgtgt gaaaatcccg
     9481 ggttccgatt ccacggcgat gctgccgccg agattttcta cctgttgttt gacaagtgct
     9541 aaaccgacgc ctcgctcgct tccttttgtc gagacacctt tgtcaaaaat gtgatcgatt
     9601 ttgtcgggtg cgatccccgg tccatcatcg ttaacttcgc agtgcagcca gccgtgacgg
     9661 tagtgcaatg ttacgctaat ttcgccaccg ggttccggcc ctaatgcttc cagcgcgttt
     9721 tctatcagat ttcccaatgt ggtaatcagc gtcgcgactt ggtcctcact gccgctgtct
     9781 ggcagcaggc tttcactgtt taaaatcagc gtatggccta aatcggtcgc gcggttaatc
     9841 ttgctgatta aaaaaccagc gataaccgga gatttgatct tacccagcag agagccaatc
     9901 tcttcctgat agttattggc tgttttgaga atgtaatctt ccaactgctt ataactcttc
     9961 agatgcaata atccgagaat cacatgcaat ttattcataa attcgtggga tcgttcacga
    10021 agtgcatcag catagttgac cagaccgtcg agtcgctgca tcagtttacg aacttcagtt
    10081 ttgtccctga aggttgaaat ggcaccgatg ataacgccat tactgcgcac cggaacggtg
    10141 ttgatcagta atagccggtc tttaatcgta atctcttcgt cgcggcgcgg cgtaccgtcg
    10201 cgtaacactt ccgagacatc taccacctgt gaccatgagt ggcttagcgt cgacagtttc
    10261 tcatcgtcct gcgacttacg gtaattcagc aattcttgtg cggcatcgtt gatcagcgtg
    10321 acctcgccgc gatcgtccac ggcaacgacg ccttctttga tagactgcaa catggcctgg
    10381 cgctgctcaa acagcgtgga gatttcgtag ggttccaggc cgaaaaggat ttttttcagt
    10441 accttaacca gaatgcaggt gccaatcagt ccgaccagca tgccaaataa taccgaccag
    10501 ataatgctcc agcgactgtc attgatttgt tgggtcacgc ggcttaactc aaggccgatc
    10561 gccaccacgc caatttgttt atgattttca tcgtagatgg gggtaaatac gcgtaaagcc
    10621 tgtgccagaa aaccgcgatt gatagcgaca ttttctttgc cattcagcgc tttaaggatg
    10681 tcatcacctt taaatggctg accaatacgc tgggcttcag gatgcgagta gcgaagactt
    10741 tgcatatcgg taacgacaat aaacagcaga tcgttgcgtt tgcgcacggc ttccgcgatg
    10801 gcctggattc cgctctcctg cggttttttc tgcaagccct gacggatttc cggcgagtcg
    10861 gcgagggtac gcgccattgc cagtgccttg ttggccagcc cgtctcgcgt catatcactg
    10921 atttgcgaga agtaaatcag atgcaccacc aatagcaccg agaacagtac cgcgctgacc
    10981 attaagatca ctgtggtact caatttcatc ggacgtttgc gtaacatgcg gtagggcaat
    11041 gaatgtctca tcagcttcct tgtgtgacaa atttcttaag cattatctct gatgaggcgg
    11101 gtaattcaaa gggagtaaga atgattggct atatagggga agagactctg gcaacggaaa
    11161 ctgccagtgc tgtatgaaga ttccagggct atgcttatag cgataatcat actgatgaga
    11221 gagggaaagt catggatcag gcgctactgg acgagggtta tcgctgttat accggcgaaa
    11281 agatcgatgt ctatttcaac actgcgatat gtcagcattc tggcaattgc gtacgtggca
    11341 acggcaagtt atttaatctc aaacgaaagc cgtggatcat gccggatgaa gtcgacgtcg
    11401 tcactgtggt taaagtgatt gatacgtgcc cgagcggcgc gctgaaatgc cgtcataaat
    11461 aagcgagggt aaaatggaaa tacgcgaagg ccacaataaa ttttacatta atgacgaaca
    11521 gggcaagcaa atcgctgaaa ttgtctttgt gccgaccgga gagaatttag cgattatcga
    11581 acataccgat gtcgatgaaa gcctgaaagg gcaggggatt ggtaaacagc tggttgcgaa
    11641 agtcgtggaa aaaatgcgtc gggaaaaacg aaaaattatc ccactatgcc catttgcgaa
    11701 acacgaattt gataaaacgc gggagtatga tgatattcgc agttgatggg agagtacaga
    11761 gtcacgatat ttttcatgct ctccgcggag tgatgcagga gagcatctga agggtagggg
    11821 gatgcacaaa gaatgggcag agagcgcgtt tttttgtccc aagtcatccc ctttactgag
    11881 caaaaaaaag aatatctcct atatgagaat catcaatcgg ggttaataag ttttgcgtcc
    11941 ccagagcatt caatattgat aggagtcata ttatggaagg taaaaacaag ttcaatactt
    12001 atgttgtttc ttttgattat ccatcatctt attcctcagt gttcttaagg ttaagatcat
    12061 tgatgtatga tatgaatttc tcctctatcg tggctgatga atatgggata ccacgacaat
    12121 tgaatgaaaa ctccttcgca ataacgacat cgttagccgc aagtgaaatc gaagatttaa
    12181 tcaggcttaa atgcttagac ttaccggata ttgattttga cctcaacatt atgacagttg
    12241 atgactattt ccgtcagttt tacaagtagc caggaaggat aagaagaaca taaaaaatgg
    12301 cactattctc taaaatatta attttttatg tgattggtgt gaacatatcc tttgtcatta
    12361 tctggtttat ctcacatgag aaaacacata ttcgtttact tagtgcattc ctggtcggaa
    12421 taacctggcc aatgagtctg cctgtggcat tacttttttc tctcttttag gagctgtagt
    12481 tgagtggacc cgctggttat cgtgaaacga agcgtctctc ctcaaaactg gatttgcgga
    12541 acgacgaacg aagcagtcgc ctgactgctt cgttcattaa agtgaaattt atttctgtgg
    12601 gcgcatcgcc gggaagagaa taacgtcgcg aatagtatga ctgtttgtaa acagcataat
    12661 cattcggtcg ataccaatac ccagaccagc ggttggcggc agaccatatt ccagcgcagt
    12721 cacgtaatct tcgtcataga acatggcttc gtcgtcacct gcagctttag cgttaacctg
    12781 ttcctggaaa cgttcagcct gatcttctgc gtcgtttaat tcgctaaaac cattaccgat
    12841 ttcacgacca ccgatgaaga attcaaaacg gtcggtgatt tccgggttaa catcattacg
    12901 gcgcgccagc ggtgacactt ctgccggata ttccgtaata aaggttggct gaatcagatg
    12961 tgcttctgcc acttcatcaa agatctccgt gacaatacgt cccagccccc agcttttctc
    13021 taccgtaatt ccgatagatt cagctaatgc tttagcagca tcaaaattat ccaggtcggc
    13081 catatcggtt tctggacgat attttttgat tgcttcgcgc atggtgagtt tttcaaacgg
    13141 tttgccgaaa tcaaacacat gctcgccata agtgacttta gtggtaccca gaacctcttg
    13201 tgccagggtg cggaacaggg actctgtcag ttcaatcaaa tcgtggtaat ccgcatacgc
    13261 catgtagagt tccatcattg tgaactcagg attatggcga acagaaatac cttcattacg
    13321 gaagttacgg ttgatttcga atacccgttc aaaaccgcct acaaccagac gtttcagata
    13381 cagctccggc gcgatacgca ggtacatatc taaatccaga gcgttatgat gggtaataaa
    13441 cgggcgagca gatgccccac ctggaattac ctgcatcatc ggggtttcta cttccataaa
    13501 gccgcgcgcg accatgaact ggcggatagc ggccagaatt tttgaacgga caacaaacgt
    13561 ttgacgggat ttatcgttag cgatgaggtc cagataacgt tgacgataac ggacttcctg
    13621 atcctgcaga ccatggaatt tatctggtaa aggacgtagt gcttttgtca gcaggcgcaa
    13681 ctcagtacag tgaatggaaa gctcaccggt ttgcgtctta aacagcgtac cgcgggcacc
    13741 gataatgtca cccagatccc attttttaaa ctgatcgtta taaacacctt ctggcaggct
    13801 atctcttgca acgtacagtt gaatacggtc accgacatcc tgcaacgtta caaaggaggc
    13861 tttccccatg atacgacggg tcatcattcg gccagcaacc gagacttcaa tgtttaagga
    13921 ttccagttcc tggttatcct tcgcatcaaa ctcttcgtgc agctggtcag aggtatggtc
    13981 gcggcgaaaa tcattgggaa acgccacacc ttgctgacgt agtgccgcca gtttttcgcg
    14041 gcgatttctc agttcatcgt taaaatcaat agcctcattg gctccccgtg tttcttgttc
    14101 agacattttg gttcctctaa atccagcttt caatttagcg tagataaaga gacagatcgg
    14161 tacactgtat tatctgcctc gactattaat aactcagttt atcttatcga aactaacgct
    14221 tacctcaagt tgatatcact aataaataac catcaaaatg gtttaaatga cttattaatc
    14281 tagatttgct agtgttataa actgaaagtt aatttattca taaccaattg tttttacgac
    14341 agttaatcgt tgctctcctg tatcatattc gtcggcgtgc taaacagaaa acgggtagca
    14401 aaggccaata cgacgatgat cgcgacacaa gccaacgtcc actctcccat ctgagaaaag
    14461 aatcgctggt aagctgcaat tgccataccg ctaatttgcg actctgtcgt ctgctgtgcc
    14521 acgacgcctg ccagccagtt ggcgaccgcg cctgttgcca gcatataaat accggttaat
    14581 acgccagaca ttttcagacg cgtgatttgc gcaatcgcca ccgggtcaat aaagagttcg
    14641 gcaaagccca ttagcgccaa cccggatatc atcacgccca ttgacgcttg accgtcagcc
    14701 gctgcatgtc gggcatcaaa tgccaacaac ataaagccac aagccatcag cagtaagcca
    14761 aaggcaaact tcagccagac gcgcaatgtt gagttgccgc ggctttctgg cgacgccagc
    14821 caggccagta caaccccagc gagcatcacc gcaattgcat tcaccgactg gaatagtgct
    14881 gtaggtactt caatattgaa tgcctgacga ttcacaaagc gatcgataaa caagctgatg
    14941 gtactgccgc cctgttgtgc cagtacccag aacaatgtcc cgacaaacat caacaataca
    15001 atttgccaaa gagcacggcg gtgttcgggg aatttgacca tcatgcgggc aatgatttgt
    15061 gcggcaatga ggcaaacgat cgccagcaaa tatccggacc agtcgttctc cagcagcagg
    15121 gtaaaaaata ctggggctaa acagagcatc accactaacc agctccatac tggtaaggca
    15181 aatttcacgc tagtgagcgc ttttttatcc atactacgcg tggactggaa atgacgatgc
    15241 ccgcttaaga aaatcaacaa accgataaac atgccgccac ccgcaagggc aaagccaaca
    15301 tgccatccat accactgagc agccaggccg caggcgattg gggcggcgat agaaccgata
    15361 ttgcccgcag catacagcag cgaaaaaccg ccatcacgtc gatgatcgtt ctcgtcgtag
    15421 agctcgccaa gcaaacagct gatgtttgat ttgaataaac cgtagccaca aataatgatt
    15481 gctagcgcca gatacaggct aaaggttgaa tttgtatcaa tgcccagcac cacatggcca
    15541 agggtcatta acagcgcgcc ggcaatcact gcagtgcggt tgccgagcag gcggtcggca
    15601 agccagccgc cgagaatagg ggtaacgtaa accagagaag catatgcgct gaacaggctg
    15661 atggcatggt tatcatcaaa accaagctga tgggtgagat agagaatgag taaggcacgc
    15721 atgccgtaaa aactgaagta ctcccagatt tggatcgcca cgatatagta tatcgcgcgc
    15781 ggctgtgagg gtgttttcat gtgttctcct tatgagcaaa aaagggaagt ggcaagccac
    15841 ttcccttgta cgagctaatt attttttgct ttcttctttc aataccttaa cggtatagcg
    15901 gccatcagcc tgacggtatg caccgtgaat atcggtttca aagcccggat agtgagcgcc
    15961 gatttcacac agcatctgca ggaactccag aaccggacgg ctttcttcgg tgatcatttc
    16021 acccggcatt accagaggaa ctcccggcgg atatggaagg atcatattgg cgttaatacg
    16081 acctaccatt tcgtcgaggt aaacttcttc ggtcataccg tgcagctctt tctggaacgc
    16141 agcatacgga gtcattacca tcgtcggcag cacttcaaat gcgcggtaca tcagatccgg
    16201 cagattgtgg tgaacaatca gtttgtggat attctgagcc agttcctgaa tacgcatgtt
    16261 ttcatagaat tcaggatctt cacgatacag agacggcagc atgtttttca cacgcaggtt
    16321 caggtcgaac gcacgtttga agtcagtcag agcacgcagc aggctcagtg ctttggtctt
    16381 atcgataccg atgctgaaca ggaacagcag gttatacgga ccggttttct caacaacgat
    16441 gccatgttcg tcgaggtatt tcgccacgat gctggccgga ataccaaagt cgctcatggt
    16501 gccgtctttt tccatccccg gagtcagcag ggtgactttg atcgggtcaa gatacatgtg
    16561 ctcgttatcg atgtttttga agccgtgcca ggtgctgtca gaacgcagcg gccagcattc
    16621 agtggtatcg atatgatccg gctgccatac atcaaagaac cagccatcag attccgttct
    16681 cagacgtttg atctctttac ggaatttgat cgcacgttca atggaaccgt tgatcagacg
    16741 cttaccagca ttgcctttca tcatcgccgc tgcggtttca gtggacgcca cgataccgta
    16801 gtgcggagaa gtggtggtgt gcatcatgta ggcttcgtta aaggtttctt cgtttacgtc
    16861 acctttaacg tggatcatgg aagcctgaga gaacgccgcc agcagtttgt gagtggactg
    16921 ggtttcgtaa atcactttcc cttctacacg gccaccgctc ataccgcatt taccttcgta
    16981 aatcggtgag aagttggtgt aaggcaccca cgcggagtca aagtggatgg atttcacatc
    17041 cagtgttttc ttgataaagt cggtgttgta cagcagacca tcataggtag agttggtaat
    17101 tacagcatgt accggccagg ttgcgtttgg tgtttctttc acgcgcttag caatggtagc
    17161 gtgctggaat tcactctgtg ggataccacc aagaataccg taagcgttac gggtcgggcg
    17221 gaaatagatt ggcgtaacat cgctcatcat catcaggtgg gtcagcgatt tgtggcagtt
    17281 acggtcaatc agaatggtgc tgcctgctgg agcagagtac ataccaacaa ttttgttcgc
    17341 agtggaagta ccgttggtca ccatgtagct gcggtctgcg ttaaagacgc gagcgatata
    17401 ctgttctgct tctttgtgtg gaccactgtg atccagcaga gaacccagtt cagatactga
    17461 aatggaaata tcagatttca tggtgttcgg accaaagaaa tcatagaaca ggctacctac
    17521 cgggcttttc tggaatgcag taccgcccat gtgaccagga gtacagaaag tatatttacc
    17581 ttcacgaaca tatttaaaca gtgctttagt cagcggaggc agaatagtgt tgatatattc
    17641 gtcagtggtc tgcttgatct tattagcaat atcttcagca gcacccagcg catattcaaa
    17701 gaagctaatc tgtaaacgca ggtcattcag gcttacatcg agagtggaat acgtattagc
    17761 gaacgcgtac aacggcaggt tctcgttcat tttgctaatt tcttcgcaca gctcgagatt
    17821 atatttatcc cagtcaaaaa taacgccgca cagacgcgca ttgttttcga tcagttttaa
    17881 taagtcgtca cggtcgttcg ggtaaacaat ctggaagttc agacgttcaa gcgcgcgatg
    17941 aagttcacgg atgggttctt ctttaaaata aacccccatg tgattcaata ttgcaataac
    18001 gttcatagtc atatctccag gtaaaaaagg cccctcccaa cacatgggac aaaatgaaag
    18061 gaggagcctc ggaaaatact tttaattaat gtgcgttaga cgcggtgtgg ttatccattg
    18121 agtggctctg gcgctcgtgc attttgcgag cgtagaacat caggataatc aggctgacga
    18181 tgaaggtacc tgccagctcg aaggagcttg cgcccatcag cgcgatgaag cagaacacgc
    18241 aacccagtac agagcagatc aggctgacaa agttgcggat gttaacgcct tcaaaacgaa
    18301 tcaggtcaac gcaagagtag aagtacggca gcatagtcag cagtactgcg ataccggtca
    18361 gttcaccgaa caggtcagat gctttaccac cggcagagtt catcagagtg atgaggatca
    18421 tcagggcagt cattttcact gcagccagca gcagaccttt tttcggaata ccgttgctgt
    18481 cgacttcacc ataaactttc gggaagttac cgtcgttagc ggcacgtaca cctgcctggc
    18541 ctaccaacat catccaggag cccagagaag tcaggcacgc aaaggcggtg aatgcagaaa
    18601 ccagcggcgc agcccagtta ccgaggatgg ttgaagcact gattgcaaac ggagcaccgg
    18661 aagctgccat tacagaagat ggatacatac cggaaagcac ctgagtcgca gcgatgtaaa
    18721 caatacctgc taaaccagta cccagcatgg ttgccagcgg aacggtacgt ttcgggtttt
    18781 taaccatacc agtacttact gctgcggatt caacacccac gaaggcccac aggcagagca
    18841 gaatactttt aatgatcgca tgaccatcag tggtatccgc agtattccag ttagctgcat
    18901 aagttgccgc atcaaaccaa tgccagccaa caatagcagt catcaccaca ggaataagaa
    18961 ccagcaccag accaatagtg gttaaacggc ttacccaggt accgccgagc atatttacaa
    19021 aggtaaatac ccagacgata gcaatacagg cgatacccgc cggaacagga tcatttaata
    19081 ctgggaagaa ggtggaaaga taagatacag cggtaatacc aatcgccagg ttaccaatcc
    19141 agttagcatg gtaataaaga acacctgtct gaaaaccaaa tgcaggggaa atttctccgg
    19201 cataagcaat tgggccacct tgttgcgggt tttttgttgc cagtcgggca tatacatacg
    19261 ccagcgacat tgcaccaata atagagataa tccaacccca gatagcaata ccaccgatac
    19321 ttgctaggtt cgcaggtaat aatgcaatac cgctccccat catattaccg gcaacaacac
    19381 cggtacaggc aaatagcccg atcttcttgg cagaactcat gctcttctcc taatttcatt
    19441 tttgaatttg gagtccgggt catgatgtat aactatttcc tgaccagacc aaactggcgc
    19501 taagaatact cacgaaaaaa ggattaatcc taaagattag gtgaaataac acaaaagttt
    19561 ctgtaagtga gaacttgagg ttttttatta acacatcagg atcgcaagtt gatatcatga
    19621 aaagataaac attcaatgtt tacaatggat tgcatgacat tctctggtta aatttatgta
    19681 ataaaaatta tgcggcaaat aaattgccgc aacatattat accaacggga acatacaaaa
    19741 actcaacaac aaatattcca gagcataaat caacccggag ttacttattc tgaagcgaga
    19801 aatttgtcga gataaggtac aacataagga acagaagtct ggaatatacc attttcaatc
    19861 cagtaaaggg tgtttgcccc tgggcgtaaa ttaaaggcgg tgagatatgc atcagctgct
    19921 tcccggttca tccccttcat ttcataaacc ttgccaagca atacataatt tagccaggac
    19981 atttcaagat caatgccagt atttatcgcc tggtaagact catctgtttt accttttacc
    20041 agagcactga ccgcttttat ttgatatata atggacaggt tattcaattc cggtagtgta
    20101 acaatgttat ctatttctgt gttcagtgct gctaattgtt tttcatctaa aggatgttga
    20161 gaatggcgca cgatatcaac taatgctttt tctgctctcg cgtaggtaaa ttctggggat
    20221 gattgaacaa tctcacctaa taattcactg gcacggttca atgatttatc atcgccatgc
    20281 agtaaataat catgtgcctg ataaaaatta gttaataacg caccacgatg aggcaaaatt
    20341 tgctggagcg tctcctgcat tcgttgtggc cacggttggt ttaacgcttt tgataaactc
    20401 tccagtaaat cattttgaat cgccagctga ttaccgttag tgatgacata acgtttatcc
    20461 agcatggttg aaccatctgc attgtctacc aattttatcg acataaagca ttgttgagca
    20521 cggtattggc gctgattaac aaacgcaata gataatgttt taccggaact gctcggttca
    20581 tcaatgttgt agttgatttt gtcatgcacc ataaaggtgg agaaggtgtt aagtgatgtc
    20641 gccaccaaat cacccacgcc tatcgcgtaa gagagctgat acggggaact ccagctgtta
    20701 caacttttat ttaccatatt aatgtcaata tcgcgtggat tgagcaaaat acgcgatttg
    20761 ctcataggaa gccgtgtatc aagagttgaa aacgctacca gtgctacaca gatacctaac
    20821 gacaacagga aaaaaaacca tacccaaaag gtagtgaatc gtttgctttt aactggggat
    20881 tgttcaggtg gcgttgcggt gttttgaatg ttaagactgt gggagggaga atctgtggca
    20941 ggaaccgcct ctggtatagg gggaggcgaa gatagcatta tttcctctcc ctcttcttcg
    21001 ctgtaccaga taaccggcac cattaattta tagccgcgct ttggtacagt agcgatatag
    21061 acaggactat cttcatcatt atcttttaat gacttacgta gttctgagat actctgcgtc
    21121 acaacatgat tggtgacaat acttctcttc cagacattat cgataagttc atccctgcta
    21181 agtacttcgc cactgtgttg agcaaagaaa accagaagat cgattaatct cggctcaagg
    21241 gtaagttgac gcccattgcg gctaatttgg tttatggacg gagtaacaag ccattcgcca
    21301 acgcgaacta caggttgttg cataatagaa actcattcga aaagggaatg atgcaatgat
    21361 aattgccaca acctattttt accatctata gatgggttta tttacatatt attggtgaat
    21421 gcaagacgtt atttttacca gcca
//
LOCUS       DAZGBC010000082        21203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGBC010000082 DAZGBC010000000
VERSION     DAZGBC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.082"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1117)
                     /locus_tag="ACHK1J_002917"
     CDS             complement(<1..1117)
                     /locus_tag="ACHK1J_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO7912160.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQGGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKD"
     gene            complement(1488..1961)
                     /locus_tag="ACHK1J_002918"
     CDS             complement(1488..1961)
                     /locus_tag="ACHK1J_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912161.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            complement(2048..3574)
                     /locus_tag="ACHK1J_002919"
     CDS             complement(2048..3574)
                     /locus_tag="ACHK1J_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7912162.1"
                     /translation="MSKKFTKTLLSSSLGGLLLGISSGVLAQELPPIKDSGLPFYSVL
                     KEADASSVIHGSTYRIYYNKSSGWGTMRYVLNGKDETLFNFDQEGNIIVLSKDNSIAY
                     TVHEPVLKDFARMAAGLRTSDKENGKHQVDEEEVRRIFNKVNNLSNTIINPEVLKQSD
                     FIASGPRPEGDPLVQASAVGEFALAVGEGAAAEEKNSTAVGSWASAHGRQSTALGSGG
                     QAYANGSTALGSYSHVDDSSVYGVALGGSSVVGENAPEGTALGSRAEVSHRNSVALGA
                     RAHTTRDNEVYIGYVEEAGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQD
                     TNTRLVVEAKKSREYTDSRTTVGVNSDGTLTSADGATKTIAVNDGLVALSGRTDRIDA
                     AVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLF
                     QPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSYNVGVNFEF
                     "
     gene            complement(3934..12321)
                     /locus_tag="ACHK1J_002920"
     CDS             complement(3934..12321)
                     /locus_tag="ACHK1J_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO7912163.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDALVNNPVKTLAGLSSFMTDVYMPPS
                     STQKELGAPPSVGDAIKNIPDGTGKNAVTDTVGKGLKVTGKSVSDGAKASDEWLTGKM
                     SPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVVGGVAKKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETAG
                     MDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAAP
                     QPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGRQ
                     GETLEGEMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLTKAKV
                     WFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAH
                     YEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRRI
                     FKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAAR
                     AFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTL
                     KTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVVRGVMSGIKDSD
                     LKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEI
                     IHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAH
                     MAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTP
                     REDGVGASSQYSRSGKPDPFKADAYDAKQFASEVRRIAGLQSVSRREMLRVGDTPRVL
                     RALGAPDLELVMPASVVHKATRPEIREHFVSVETLADLPQLLTDPVVVMRSKTESDAL
                     VSLIQATDTKGNPVIVAIHTHVKGDGFAEINKIASAYGKDKRSALQDQLDTSLLYLNK
                     EKAAEWLHAVGLQLPEANTINGSSGFKILHPEDIRKGPYYSRTSSLTPEDTIASRFVR
                     QMQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKL
                     LADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGK
                     QAQYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGVVSLP
                     RTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNP
                     DKDYWQVFTDDRPDTMRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTY
                     YIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTA
                     VMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGTQWQKVWK
                     EFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVE
                     NALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTAN
                     LVRTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWY
                     DKVPSHVKERNLVIMKSMFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRA
                     AGNVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENM
                     PFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGG
                     TGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAE
                     LKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMV
                     KSVERDMKIAVDRFNREYNKKVGVE"
     gene            complement(12407..13669)
                     /locus_tag="ACHK1J_002921"
     CDS             complement(12407..13669)
                     /locus_tag="ACHK1J_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912164.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEAIGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKEASKVVAQAIKDGRDAQAANDEFLAAYDSL
                     PHQQKVQQRKTQQLLQKWQPWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
     gene            complement(13680..13952)
                     /locus_tag="ACHK1J_002922"
     CDS             complement(13680..13952)
                     /locus_tag="ACHK1J_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO7912165.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGATIGATVGSAVPVVGTAVGAVAGAVIGGIAGALF"
     gene            complement(13962..14408)
                     /locus_tag="ACHK1J_002923"
     CDS             complement(13962..14408)
                     /locus_tag="ACHK1J_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912166.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(14411..15061)
                     /locus_tag="ACHK1J_002924"
     CDS             complement(14411..15061)
                     /locus_tag="ACHK1J_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912167.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGGAKTPGTGANAIQHQAQNWRL"
     gene            complement(15155..15556)
                     /locus_tag="ACHK1J_002925"
     CDS             complement(15155..15556)
                     /locus_tag="ACHK1J_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912168.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(15613..15753)
                     /locus_tag="ACHK1J_002926"
     CDS             complement(15613..15753)
                     /locus_tag="ACHK1J_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7912169.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(15984..16718)
                     /locus_tag="ACHK1J_002927"
     CDS             complement(15984..16718)
                     /locus_tag="ACHK1J_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7912170.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHSRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            complement(16809..17426)
                     /locus_tag="ACHK1J_002928"
     CDS             complement(16809..17426)
                     /locus_tag="ACHK1J_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912171.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(17432..17710)
                     /locus_tag="ACHK1J_002929"
     CDS             complement(17432..17710)
                     /locus_tag="ACHK1J_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912172.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(17725..18993)
                     /locus_tag="ACHK1J_002930"
     CDS             complement(17725..18993)
                     /locus_tag="ACHK1J_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7912173.1"
                     /translation="MSKKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(18990..20615)
                     /locus_tag="ACHK1J_002931"
     CDS             complement(18990..20615)
                     /locus_tag="ACHK1J_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912174.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSELPI"
BASE COUNT         4921 a         6207 c         4588 g         5487 t
ORIGIN      
        1 catccttcag aacaaccgtg cccttacccc caaaggtgat atctttcccg tcattcagac
       61 tccctcccct gaagccgttc atcttccata cctgcccgcc ctgagaaagg gttccctctc
      121 cgcttccctg actgtacttc cacacaatgt caccaccggc aactgccggc acaactgtgc
      181 ctgaattgtc ctgctttatc gtgttattca catcccctgc aggaatcacc gtaaaccagt
      241 tcgtcttccc gttctcaccg gcataaccat gaagtacagc aacaaatacc cactgcttct
      301 tcaccgcatc ccaggcaaaa agcggtgagc cactgtctcc tgatgtcccg taagacgcca
      361 gcggaccatt aacaccctgg taaagatttc ccgggtttga caccagactc cagtcagaaa
      421 tgaccggact tcccaccgtc ccgccagtcc tccagacgta agcatccgct attctggtca
      481 gcgcaccgga agcagctccg gtgtactgag ttccactccc catacgataa aacaccggat
      541 aacgactgcg gttctggtat acccccttgc cggttccggc atccgtgacg ctcaccgggg
      601 caacttctgt caccagtttg ttcagccggg gagtatgaaa atcacgccag tgctcattac
      661 ggtcaacaag gcgatatgtc gtatcgtccg cataaccaaa acgcaccgta ttgtagctgc
      721 cgttatgctt aacccccgcc acatactgcg gagaaatcag tgttgccacc cccagtatct
      781 ggtccacaac actgaaatcc atcatcggag ccttgtccag ccgcccactg acagccccat
      841 tcttaccata aagcggaata tttgtggccc cggcatgaaa cgcccctttg ttttcagcaa
      901 aatccctgta ggtctgccat ggcagagtat tcccgacaac ggatgccagc gcaggagaag
      961 aaaacagaac gccggcaacg gctcctgagg ccagaagaag acgggaaagc atccccgcac
     1021 cgggaattcc ggacgacttt ttcctgcccc ccaccgtttc cggtacagca accagttctt
     1081 ttctgtcatt aactctgagt gagtatattt tgttcatccc ctggattccc tgtattttct
     1141 cccggacaac aataaacagc gggcacaatc atcatactaa aacgaacttt gactgataaa
     1201 gataaaatca ccattaacca attgatttaa atacataaac ccacaggcat ttaacaagtt
     1261 ctcagttatc agaaagttcg aattaaggaa gtaaactctc ttccgttttc acctcattat
     1321 cagagcggcc agcacacaga atgtcaccac accccccagc catacaaaca gcatgaagca
     1381 catcgcaccc gctgtgtatt tccgctttcc gcatctgatt tttttctgtt ccattctcac
     1441 tgccaccgtt ccactctcat tacccgactc acgaatatgc attccggcta cgcacgtcca
     1501 accaggtgct tcacactcct gtacttaaga ccatttttac caagtcccat atacagaaag
     1561 gtgttcaccg tcttcgtgtt tatccccatt tcacgggcaa gatggcaggg ctttgactcc
     1621 cggtacacgt atccaaacat caccaccatc tcggacatcg tcagaggtcg cgcatacgta
     1681 ttatgtaccc actcaccatt ccggattttc ctgtacgtct ccaggcgccc cgtaacatct
     1741 tccgcatcaa tctgaagata acggcgtccc tgaaacatgc agcacatcag cctgccaagg
     1801 agggcatcac agattatcac ccatgactca tcatgtgccg gtacactctt cctgttctct
     1861 ggtgtaaaaa actccctcgc cagtattatc ctgtacccct caagcatctg ctgcaggcca
     1921 tactccagat aacggttgcg ggtcgccaga atcactttca tcgctgtcac ctgtttcatg
     1981 ctcacatgag aaaaaacggc aggcaaaact ccacgcctgc cgtcatgctt catgtcactg
     2041 tcagcaatta aaactcaaag ttcacaccta cgttgtagga cacatcgcca tcactcgctg
     2101 ccacacctgc tttcgctgct gtctgctcat tgaaacggta accaacaccc acggcaacag
     2161 cctgtttatc actgtacccc cccagggcag cagtggcgtt gaatttcccc acactgtacg
     2221 gctggaaaag ccccgccagc gctgcactct gtgctgctgc acgcttcatc tcttcgtgat
     2281 tctcacgaat ttgacgctgc tggctgttca ggcgtgcatt atgttcctgc aactggcgag
     2341 tattggactg aatagcctgt gtatttttag taacccggcg gtcaacgcta ccaactgcag
     2401 catcaatacg gtctgttctg ccggacagag caacgagacc gtcattaaca gcaatcgttt
     2461 ttgtagcgcc atcagcgctg gtcagggttc cgtcagagtt cacaccaact gtcgttctgg
     2521 aatctgtata ttcacgagac tttttcgctt cgactacaag gcgggtattc gtatcctgag
     2581 ccagttgctc aacactgtct tttacacggt caacctgacg aacggttgct gcatctgagg
     2641 gacgagtacc gtcactcagg ccaccaagca cacgagtttt ataagccttg cccgcttctt
     2701 ctacgtagcc gatataaact tcattatcac gggtagtgtg cgctctcgcc cctaaagcaa
     2761 cactatttct atgagaaact tccgctctgc ttcccagtgc agtgccttcg ggggcatttt
     2821 cacccacaac agagctgccc cctaatgcca cgccataaac ggatgaatca tccacatgag
     2881 aataacttcc caacgcggtt gaaccattag cataagcctg ccccccactt cctaatgcag
     2941 tactctgtct cccatgagca ctagcccatg aaccaacagc tgttgaattc ttttcctccg
     3001 ccgctgcacc ttctccaaca gccagagcga attcgcccac cgcactagcc tgaaccaaag
     3061 ggtcaccttc cggacgtggt cctgatgcga tgaaatccga ttgttttaat acttcaggat
     3121 taataatagt atttgataga ttattaactt tattgaagat ccggcggacc tcctcctcat
     3181 caacctgatg cttaccgttt tctttgtcag acgttctgag gcctgctgcc atacgggcga
     3241 agtcttttag cacaggctca tgaacggtat atgcaattga gttatcttta ctcaatacta
     3301 taatattacc ctcctgatcg aaattaaaaa gggtctcatc ttttccgttc agaacatatc
     3361 gcattgttcc ccaaccagag gatttgttat agtaaatacg ataagtacta ccgtgtataa
     3421 cagacgaagc atctgcctct ttcagcactg aataaaaagg caaaccagaa tctttgatag
     3481 gaggtaactc ctgagctaaa acacctgagc tgatcccaag cagaagccca cccagagaag
     3541 atgacagaag tgtctttgtg aattttttac tcattatgga tatcctgtta aattatctgt
     3601 atacaaatca gccgcaacac ctcatactca taaagaaata tgattaatta cggctttatt
     3661 caggatattc tgaaaatgat cgtgattatt aattttaaat aactaggaat tatcttacta
     3721 tattcttggt gtattcactc ctgagacata gagcaacgta ggaaccgaaa taagaaaaat
     3781 cttaccctca tcaggcatga catttcacat ctgcttacac tgtaatttct gatcaagatc
     3841 acaacattat ccgaatttaa atgttcagtt catctacaat atttccctgc gtgactttat
     3901 atcagccccg tacatccgta cggggcatgt tctttactcc actcccactt ttttgttgta
     3961 ctcgcggtta aaccgatcca cggcaatttt catatcccgc tcaaccgatt tcaccatcgc
     4021 cgactgttgc gccagactga gagtactgtc ggcgtaaatg gcatcacgct gtttacgcaa
     4081 atccttcagt ctcttccggg tatcctgcat aagcccgttc atcgacaatt ttccgttgtt
     4141 ctcgtcaatg aacgccgttc tttctgcacc ggtcagactc ttcagctctg cgtgatactg
     4201 cgcgacctcc gtcatccggt cgtacatctt ctgctggtca gcatacggca tcacctcacc
     4261 tgaaattttc cccaggaagg gcacctgctg ctccggtata tcaataccat tcagcgattt
     4321 caccgccgca tccgtggttt tagaaatgaa gcgcccggtc ccgccagaga tatagtcaat
     4381 ccagaatttc agcgattccg gtgtgatatc caccgcgccg ggacggtact ggctgccacc
     4441 agagaacgca ttcagccagg atgcaaacgc cttgtacgct tctggcgttg aacgtcttcc
     4501 cagctggctg tcaggttttg gtgtaccaaa cggcatgttc tcctggtaaa tctgcgcccc
     4561 catgaagttt tcattcatgg caaggttcgc aaacggacgc agaatggtcg gcgctgcatt
     4621 tttcagcaat gccccggaca gtgtttccga cgtctcactg ccaatcgggc tgaatgcacc
     4681 aagcacacca ccaacaacat taccggcagc acgggacgcc gtcaggtcac ccgccgccac
     4741 accttcagcg gtatgcccga gcaggaagaa aacgttgtac ccgtaaggca gaggaatact
     4801 ccagtactct ccggccttgc ccccgaacat cgatttcata atgacgaggt tacgctcttt
     4861 cacatgagac ggcaccttgt cataccagtt aaccccgtca tcatcctccc ccgcaacact
     4921 gcggttaagc gagccaagca gataacccgc tcccacagct gcaagcgcga ttttctgcgg
     4981 tacattgaga ttcttccagc gaaggcgctc cagtaacggc ccgtcgccat taagatgtcc
     5041 gagcgttctc accagatttg ccgttccctg aatactggcg ttggcgaaca tgtacagcga
     5101 gttcatcagc gccccctgct caccacgacg gttaaagttc accgtcatgt ttttggcaag
     5161 agacgccgcc tgctggcgtg acaaaccggc atcacgggcg tgtttataag ctgacagacg
     5221 cagtgcgttt tcaaccgcgc cgttggcatc ctcgaccagg ttcaggaacg aattccatgc
     5281 accgatactc tggcctttcc atcccccctt cgccagtgag acaaggcgat ccatttcctt
     5341 ctgctggcct tcaaggtcac ccatgttaaa ccagccggtt ttgcctccgt cctcaacaaa
     5401 ctctttccac accttctgcc actgtgtacc tttgcccgtg agagttttgc ctcgcagact
     5461 ggcgtacacg gctgacattg cagaacggct gtctttgact acagccaggg cagataagtt
     5521 atccagccct ttcagtttac cgtcgctcct tccctgctct gccttcaggt tcatcaccgc
     5581 cgtctgcacg tcacggatga agttactgac caggaattcc gggttatacg acgtgttcac
     5641 cgttgccagg aagcggttaa ctttccccag cgtacggatt acggcgttac tggtttccgg
     5701 tcccatattc ttcatcgcac gcatcaggcg cgggtcatgg agtttgatgt agtacgtttt
     5761 gccgttcttt ttggtggtga agtaccggtc tgccatcatt gccatcgcca ccggacgctc
     5821 gacaacttcg cgaatggttt caccagtttc ctggtccttg cgctctgcaa tcacacgcat
     5881 ggtatccggt ctgtcatcag tgaatacctg ccagtaatcc ttgtcgggat tatcctgcac
     5941 cagtttcagg aaggcgttac ccacttcgtt tttgcgatgg cggatcagcg attcgctcag
     6001 gtcctgtatc gcctgagtgg acggagactg tgcacgggat gcacgcccca tggcctgcct
     6061 gctttcacgt ccgccgatgg tgaagccctt acctgtacgg ggcagtgaca ccacaccgtc
     6121 aacatcctgg cctttcaggg gaacgtagta acggtaggcg ttctgccagg catccaccac
     6181 accgctctct tccagtccgg cctcacggat aagctcacgg cgacgggcca gcatatcgtc
     6241 aacaatccct gccagacggt catactgtgc ctgtttgccg ctgttacgca cacgctgcat
     6301 gatttccgcc gcctccgcgt tggtcatccc cgaaccgccg tccggcattt tcgggttgat
     6361 tttcgcgata tgcgcgttac gttccggcgc gtgacgggcg tagaggtact catccagatc
     6421 ggcctgcgca atcttatagt ccgccagtaa tttagccagc ggctgaacat agcgctcctt
     6481 catcacgttc aggtcgtttt ccgccttccc gtggaagagt tcttccgcca tataagcgtt
     6541 gttactgtcg tctatttttc cgccagtttt acggatattc tcctgaacag ctttcagcac
     6601 ctggaattta tcctgcatct ggcgcacaaa acgcgatgca attgtatcct ccggcgtcag
     6661 actgctggta cgggagtaat acggcccctt gcgaatatcc tcaggatgta gtattttaaa
     6721 tccagaggag ccgttgatgg tgttcgcctc gggcaattgg agcccgactg catgaagcca
     6781 ttcagcggct ttttctttat taagatacag caaagatgta tcaagctgat cctgcaaagc
     6841 actccttttg tcctttccat aggcactggc aattttgttt atctctgcaa atccatcgcc
     6901 tttcacatgg gtatgaattg caacaatgac aggattccct tttgtatcgg tggcctgaat
     6961 taaagatacc aacgcatccg attcagtttt cgaacgcata actactaccg gatctgtcag
     7021 aagttgaggc aaatcagcca gtgtttccac tgacacaaag tgttctctga tctctggtct
     7081 cgtagcctta tgcacaacag atgcaggcat aaccagttca agatctggtg cccccaatgc
     7141 gcgcaaaaca cgaggcgtat ccccaactct gagcatttcc cttctggaaa ccgattgtaa
     7201 tccggcaatg cgacgcactt ctgaagcaaa ctgctttgca tcataggcgt cagccttaaa
     7261 cggatcaggt ttaccactac gggagtactg agacgatgca cccactccat cctcacgcgg
     7321 tgtgtaacct tcccgcacac gctggcctaa cgtacggatg gtctcgcgaa caagtctgat
     7381 atcgttcagt tccgtcggtt tcagtaaccc cgtacgacgc agtacccctt tgaccagggc
     7441 aacaacacgc tcccatgccg ccacaaattt attcggctgt ttctctgcca tatgcgccag
     7501 aaattcaccc gcctgcactt ccggtgattc cttaccatag gacgcatcaa ccttacgcca
     7561 ggcttcacgg atggtggcgt tatcactgtc gcgggttttc agcacggttt tgataatcgt
     7621 ctgatattcc gctggcgtga caacatgctc catggcatgg tggataatct cgtgacgcaa
     7681 cttctcgcgt acggtccgcc cgtcagggat gttatccgcc accaggacaa tttctcgttt
     7741 atccggacga tagaatgcgt gcaccctgcc gtaaccatcg aacaattcgc ccgccagcgc
     7801 ttcagcctct ttctgtgact tcaccacacg gaccttcaga tcactgtcct taatgccgct
     7861 catcacgcca cggacaaccg cttcaacctg cgggaccggg ctgcctttgg cttccgcact
     7921 gcggttaaca tccgaaatga gattgccttc aggtgtgcgg gtcacgccct tacgggaata
     7981 aaacgcaact cccttgtccg tctcacgggt tttcagcgta ctgaacaggt gatcgaatgc
     8041 atcacgaata ccgccatcca gttccgcatt cgtcggataa gcccaggtgt tatctgtgtt
     8101 gtgctcaggc gccttacgga tattgaccag ataatcattc tccacgccag ccatacgcgc
     8161 cttatcctga acataacgct caaaggcacg tgccgccatt tcaacatccg ttgaccagta
     8221 cggttttgag cgcacctcat cgagaagcgc tgaacgacgc ggcatgtcac tgtttttaat
     8281 ggcctgaatc acacctttaa aagcgtcgta aacctcctga cgtaccggat attcagcatc
     8341 aacatacctg ccgtctttaa atatgcgcct gacacgctgt gcttccgtca taaagtcacc
     8401 acctgacgta attttcccgt cattggaaac gtcataacga ccaaaataat tatccagaga
     8461 atggaaccat tcgtgcgcca gcgcacccgg tccgttacct tttgtcaggt tgattgccac
     8521 ctcacccggc tcatagtgtg ctgccgcctt acccttacca cgggcaccaa atgccaggcc
     8581 aagacgaccg ttcagggaaa gcgcttttgt cggtacattc agtacttccg caaggtcatg
     8641 cagcgagtca taagcccggt tcaaatcagc ctgacgacgc ggaccttcca cataattacc
     8701 aaactgcaca ccacggaaac caaacgcatc actgaactgc tccggtgaaa cattcccctt
     8761 gcggcgttct ggtccggtac ggtcgcggtt ggtggcgttg cgctgctcct cacgcgaaat
     8821 ctcccgcatc tccttcacat gacgaacaag ctcatcacga tgtgaatcaa tgtacttacg
     8881 cgcatcactg gctgacttaa agccacctct cacccgcatt ttgtttttac cgtaagcgat
     8941 aaaaatatcg ccactgcggg tattccggta aacgtcaaag cggattttgt catccggtga
     9001 cggtgctgtt ttttcatcac ctttcgcctg tgatttttcc tcctgctctg caaaccagac
     9061 tttcgccttt gtcagtaatt catccctgct ttccgagaaa aagaggttac tacccttatt
     9121 gtccttattg cgcagtgaat aaagtttctg tggcggatcg taacgcttcc ctcctgccgc
     9181 ctgatacaca cccgatacca cccgataagc agaagccctg tccatctgtg agggtggcag
     9241 agtgcgtaac agttgccagg tgtccgcgta acgggagggc attctgcctt ccatccattc
     9301 tgcgaggcgt ttcgcgctga ccgttccgtt cagcatttcc gatacactgt gtcgtacttt
     9361 ttttatgctc tccccccagc ctgccgtatt gtgtttcgtc ttcgccggaa tatcgctacg
     9421 atacagcgct atcattgcca gggtatcagc atcggcacct tcgttcgcca gttttgcgta
     9481 gtccggtttc gggaacagtt tgctcagcgg ctgcgtggca taatccctgt cttccagcgt
     9541 tttacccagt gtttcagcaa gctgtgcata acggtgtttg gctgcccctt taatttcctc
     9601 gccaaagtct tcaatttttt tgcccctgac ttcacctccg cgcacggtag cttccctgtc
     9661 agtagagaca gttgtttcag attcaggtgc gattgtctca ggctgcggga tgtcacgatt
     9721 tcctccggcc tgtcggcgta cttctgcctg ctgtgcaagc tcaggcagtg agtcccgtac
     9781 agtgcgggga agtgattcag gctgaggcat acgaacatta cgcgcaatgt caggagccgg
     9841 tagcccttca cgtaccggtg ctgtcgcgtt ctgcgcatcc ggtgacggca gaccacgacg
     9901 aaccatttca ccctcgagtg tttccccctg gcgaccagcg gcgttttccg gtgccggagc
     9961 ttgtcctttc tggaaactct ggcccttaac ctcaccggtt gtggtaaaac gaccaccacg
    10021 tcctgcctga ttctcatccg gcgtacgcgc cacctctccc ggtaacggat atccctgtcc
    10081 gggatgaata tcgccgggag cgggcaggcg tggacgctca gttaactcct gtgctgtcgg
    10141 accggcatca ccttcagcca tctgtgaacg tacaagctcc tctgccgtcg gcacgttgcc
    10201 acgggcaagg cgggcctgta cctccgtatc atcccccgtg aaaccacgga atcgtgggtc
    10261 acgcatgaac gcgggctgct ccatcgggtc agcatcagca atacgctctc cgtcagccag
    10321 ggtgttaatg gtttccgttg ccacatcttc agagaacgca ggattatcca tttcaacggc
    10381 atactgattc tcgcctttct tcactacgga cggtttcagg ccggtggcgg cggcattacg
    10441 gaaaacatca ctccccatgg cgcttttctc atccgtgaaa taacggttgt ccgggcgcac
    10501 cttttcaatg cgttccgctg gtctggacgc accattcccg ttaatttcaa tgcggacttc
    10561 attcgggtcc cggaaccgca ccgtcgggta aatgctcccg ttaccatcct ccgcattttc
    10621 aggctgtgac tgtgcttccg gcgtcacttc ctgttgtggg cgaatgactt ctcgtattgc
    10681 ctgaaactgt tgttcttcag tctcggtgcg cccctctttc tggctgagtc tgcggtattc
    10741 ctcaagcagt tcagaacgcg gcttcgcctt cagctcattc atcacagcct gtcgcttcgc
    10801 ctgttcgtcc agttcattca acaactggct ggcagcttcc cggcgatgag ctgcggatgc
    10861 gtcaccctct gttgccatat ccgcatcagc atactgctcc agaagctgct cgcgattcat
    10921 gccctgcatg gattcacgct gctgcgccac cggatcaaca gattctggtt gcggggctgc
    10981 gtcgtcctgc tgcacggttt cagcatcacg catggcggtt tcttctgcgg cctgacgtct
    11041 gccacgatat ccggcaaccg caccggatgg agcccccatc gcagcaccaa aggctgcacc
    11101 ttcgatcgtt gcgtcagcca cgccctccca cggtgacaca tccattccgg cggtctcacg
    11161 caatgccgtg ttttcctgat agcgtgaata gccgccctgc gccgcattaa tcgccccctg
    11221 ttccgtggca tttctgacaa tgccgctttt aacggtcttc gctgtgcctc gtgtcaccag
    11281 attaaacagt tgtgcgtcac ccagttttgc cgccatggca ttcacagcca gcaattcagg
    11341 atcggttgcc agctgcgcgc gcacctcatc ggcaacacgc tcttttgcca gatccatttt
    11401 ctggcgatca gtaagctgtg cgtgctgcgg gtcggcgtca atggacaaaa acgtctgctg
    11461 aaatttcggt gactgcgcca gctcagagta atccgcatta agaacagcat ctgctgccgc
    11521 cattgcactc tgcccctgtg cactggctgt ggaatgggtg atcaggcccg cctggaataa
    11581 atccggcatt tttttatcga cagcttctgc tgccagtgcc gtggctcttt ccggctgcat
    11641 ccctgccgcg atgtattttt tctccagccc ggcggtcagc atttttcgca gtgtgacatc
    11701 acccaccttt ttagccacac cgccaacaac catatcaggt acaagcgcac caatcaggtt
    11761 tacacccttc gccacccaga ccgcagaatc atcatagcct tcggtcatcg gcgtattcag
    11821 cgcacgaaca gcacccggcg acatcttacc ggtcagccat tcatccgagg ctttagcacc
    11881 atcactgact gacttgcctg tgaccttcag acctttaccg acagtatcag tcaccgcgtt
    11941 tttccctgtt ccatcgggaa tgtttttaat cgcatctcca acagaaggtg gtgcgcccag
    12001 ttccttttgc gtggaggaag gcggcatgta aacatccgtc atgaaagacg acaacccggc
    12061 aagcgttttt acaggattat ttacaagtgc atcaccgagt ccacgggcca tttcccctgt
    12121 tccccggacg gactgggcga aacctttacc cattgttggc aatacatcac cgaggctgaa
    12181 cgatgtgctg tgatcaatgg cattctgcgg gttgctgaaa aaatcctgcc agtaattttc
    12241 tcccggctgc ttaatgttca ggctgctgcg gttctggtta cccaattgcg cttcagggcg
    12301 ctgctcatct gaaaaagcca tacagactcc agtaaaaaaa agcccgacac gatggtcggg
    12361 cgtaagtgaa cgatattaac ggggagcgcg ttactgccgc atatctttac ttcatccagc
    12421 ctttgatctt ctcggcagct gcggaagcgc tataatccag cgccttttca tgctctgcct
    12481 ttttatcccg gaggtgattc tggtaataaa tttccacctc cttttcacga ccaggttcca
    12541 gcaaagcagg cgcattggcg gcagcctctt ttacaaactc ctgtttaacc ggatcgcccg
    12601 ccgcccacgg ctgccacttc tgtaaaagct gttgcgtttt ccgttgctga actttctgtt
    12661 gatgcggcag tgaatcatac gctgctaaaa actcatcatt cgcagcctga gcatcacggc
    12721 catccttgat cgcctgagcc actactttgc tggcttcctt ctgaagctct ttcagagaaa
    12781 gtccctgctg cttcggcatg aaataaccat aatttttcga tatctctgca aatttgctgc
    12841 gatccctgac ctgagcgata gccttatcaa cgggtatcgc cagcacggtt tgatcatcag
    12901 ggtgcgcact gccgtattct gttacaggtt tatgcgcggt ggagccatcg ctgtatgtga
    12961 gatcaaggcc aataagtacg tacccttcct gctgcgccgg tacgatactg ccaatcctgg
    13021 cctcttttat cgttttttcc ccggttgaat caggcatgcc aatacgctgt tgtagttccg
    13081 gggcaaacac gccggaaagc acatcgagat tttccggggt attcagcgaa tcgatcgcca
    13141 tgtccggctt atcaccaaag attttttgca ggttgatcac ggcctgaccc gctttcgctg
    13201 catagccttc gtttgacata agaattaatg gatgtccctt tggcagctgc ccgaacagac
    13261 gcgttgcagc ctcctgatcg ccagcctcaa ttgcttttcc aatcgcttcc attagtggct
    13321 ggctatgggc aatcatatcg ttatactcaa gccgtcgctg gttgtatttc tgcaactgga
    13381 gtcgttgctg ttccatcccc agcgacgcat tccggtaatt ctggttggcg ttaaactccc
    13441 tttcctgcag tgtgtaattg cggtcatcaa ctttggctct gtaatcaaag ttccgctgat
    13501 caacgtcttt attatgttca aactgagact gcgcaaattc aaaatcgcgc tcgttatttt
    13561 tctgttgctg agcaagctgt gcttctcgca aaccaagctc cttacgacgg gtcattgcct
    13621 gatcaacagt gctgaatcca gcaagtaacc cctgtgcaaa tccgctcatt caccactcct
    13681 taaaacaaag cgcctgcaat gccgccaatt actgcaccag caacagcgcc gacggctgtc
    13741 cccactaccg gtactgctga accaacagtc gccccgatag tggcccccgt accaatgccc
    13801 gtaccgatat tctgtttttt ctgagctttc tgttgagcag ccagttgttt atttgatgcc
    13861 tcaatctctg tacgtcgtct gtctgcgtca cttattccct gtaatgcctc acgccgcgac
    13921 tgatttgcaa tatccagtaa accgtatccc atattgcccc cttacgctgc caccagttgt
    13981 ccgccaacac tcagtttctg tcttgccggt gcagaagccc ccgtcagaat attcatctgg
    14041 cgatcctgtt cggcttcacg gataccattt ttcgcgccag caattgccag ggcattacgt
    14101 aaccccagcg tattactgtc gggattatcc gggcggttta ccccgtatct cgccatctgg
    14161 ttatcctgcg ccatctgcgc tgtacggaga ctggaagtgg caaggcctcc cacccgtgca
    14221 agctgtgcat tcatcagact gttgttctcc ccaaggtcag ccagccttgc tacacggggt
    14281 aaatatctgg tgcgccagtc gtcgtattgc tggcgggtca gcgctgcgga tgtctgccag
    14341 tccccctgag ggcgggctgc tccggtataa ccgtatcttg caagtgtttc gtatttaccg
    14401 tactccataa tcacagtctc cagttctgag cctgatgctg aatggcatta gcgccggtgc
    14461 caggtgtttt agcaccgcct ccgctaccgc cagccttatg catcgcatac gcacctgccg
    14521 cacccagacc agcgccaaca agagaggctc gcccctgctg tttcgtaaac gccgcctgtg
    14581 catccgattt agcttttgcc agactgctgt ctgccagaga gttaaaactc tgtaacgcat
    14641 ccgccttctg accagaaccg agagcagcaa catcctgtag cccggcaaca tacttatctg
    14701 cctgcgacac ctgcccccgt gtggttgtgt caatctgccc ggtcacctgg tcgctttgat
    14761 ttgcatccat taccgcatta aaacgaccac tggatgggtc aacgccggac tgagcaagat
    14821 tacccgccag ctcccttcgc gcttcaccaa actgtttctg ataaccaaga tttgttgtgc
    14881 cagcgatatt ttcgtactgc tgctcactgt taaggtcatc gaccttttcc atgaagttat
    14941 cttctgccgg acggaggatt ttttgataat cctgccaccc tttccaggcc acttcttcct
    15001 gcgctatttg cgctgctgtt ggttttactt tggtatcacc accgccttta cttccaccca
    15061 taatggcccc ctggataaca aaaaaccctg ccggagcagg gtcagaatgt gaattacaat
    15121 gttggtttaa acacaatgat gaaaactata gcagtcagac cgctatcctg aacaccatga
    15181 agccatcctc atcatccggc attcgctcaa agcccagacg ttttcccagc cggataaatc
    15241 cccgccttgc cgtatgaaat tcagcccagc gtccgccagc cagacgggtt aatgtcttca
    15301 cctccggcag ataacgctca acgctgttac tccccgtaca cacgcccagc aacaccagaa
    15361 cataagggat accatcagca ctgagcacag aacgcagcac cagaaagcca tcaggtgcct
    15421 caaaacaaaa cgcctgcttt ttaaggcagg cgtctttaac ttcattcata aattcagggt
    15481 tgcgggaatt tctcaccaca cgctgcatat accggagaat tttatcgttc attctctcac
    15541 ctgaaacggg tgccatatcg gctgaacctc agcaaccagt tgacggggac tttcgtcccc
    15601 gccgcggttt tcctactgct tacactgtaa gaacgccgca aactccgctc cccacaaatt
    15661 cagccggaac tcacacaacg agccgtgtaa catccagatg gtgaagataa ccgtcataca
    15721 aattgtgacg gtgataagcg atttttgcga catagcgctt gcctcctgag tggagaggcg
    15781 ctaaccttct acttgtcaag gtacgaagtg ttagggcctc gggttaacgt taagttgact
    15841 cggggccttt ccacatcagg ccttcaggtt ctccctccag ccatcagccg aaaggcaccc
    15901 acgcataatt tacggttttt gccctgtacg ggcaataaaa aacccgccat cacagcgggt
    15961 agtaaacggt taccggatac aggtcagaat ttcaggccaa tgccagcagt aacgccactc
    16021 gtgcgccagt cgccagagcc ggaaccttcg taagccacat ccagtgtaac actctcattc
    16081 aggttaaact gtgcacctgc agcccacgca agggaggttt ttttcgtgct gttgctttca
    16141 gaaaatccgc cactactgtt aatattgtcc ttaattttca ggtcagcgct aactttagca
    16201 acgcccatcc ctgccatcgc atataaactc atgtactgat taacccgcca tgaaggcccc
    16261 gctaacagac tccagtaatt cgccctgata tccgttctgg cagaagctgc cggattctta
    16321 atcttcctgc tatgatcggc tgactgcaca tcaataaatg tctgtgagtt agtgagagaa
    16381 cgcgtccatg taaaagaggt gataacgcca aaatcatccg ttatctcgta gcgatacctg
    16441 atattaatgc cctgtggatt tttatccttg ccttcatatc cactgacccg accatctgta
    16501 tattcgccca atgaagaaaa gtagtttctg ttgacgaaat gactgaaagt ctccctgtta
    16561 tgagcagtcg catcctttac aaaatccttc agtcccggaa actgaaagtg cgcatacccc
    16621 agagaaattg tatggtttcc ttctgctgca tgtgcagata aatttacaca ggcaatcccg
    16681 gagattgcac acacaaccag tgttgctata ctcttcattt aagatatcct gtattcagat
    16741 aacacgctaa actttttaat aatacatctt tttagacatt ttaaatgaat attaaagact
    16801 cgcttcaact aatcatatac tgatgtatta ataaccagga ccttatctat atacggcttt
    16861 cgaatatccc atgttgcacc accggaataa tgctcacagg aatatatttt atttcctgta
    16921 gcaccagtgg atgtggtata aatcggtcga tcataaggag ttcttttcca gttataatac
    16981 ccgaccagtg caggcatgat tgcgcatgga tatccaaggt ccttttcaaa tttgatatca
    17041 acaggtatta acttcgcgtc aagcagcatc atttcgccat gataaatcat ctcaccgtcc
    17101 gggttataca tggcgatacc atactcagaa ggtggagtaa ccatattcgc gaaagcataa
    17161 accgtcgtaa caccagggtt cgttcccctg acaattacat gaagccttag tgcatgatat
    17221 ccatcaatct gttcatgcgt gtacatgaca tcggccttct tctctgttct gataaagaaa
    17281 aaacaacttt tgcctgacgg gattgatgtt ttaaaaaaag acttttcagt cgccggtatg
    17341 gtgcctttgt tgatcagaca ctgcggcgta aaacctggac ttatccatac gctgccatcc
    17401 ggcttcataa tgctcaaacc atacatgaca cttatcccca gaatgtataa atataagatc
    17461 ccataccctg ctcaagattc gaccacgtca ccgtattgcc attaatggtt atcttcggta
    17521 ctttccgatc cgcaaataca ttattccagg gaaataaaca acatacagcc tgcaatgatt
    17581 tcccgtcggg tttattcgtg tacgtctttg agccagactc cgctgtaaat ctgtccagga
    17641 aaaataccgg agtcaacacg cccgtaacat taacgttatt tctgttataa atggcaaaac
    17701 cgtattccaa tactcacctc ctgatcagcg taatctgcct atgcgaacag ccagtcgtcc
    17761 attctgatca taaacctcaa ttttatcatt gcggatcacc agtcctacat tctgattaga
    17821 gtaacgaatt gtcagttgcc cttgtgacgt aacactgaaa aggcctccaa tattcaggtt
    17881 accctgagaa tcaacctgaa agtttccgtt ctgaataacg gcactccgga taactggcga
    17941 agtgatactt accccggctt ttacctcatc cgccacaacc ttccgcgaca ccagtgtttc
    18001 aatcaccgcg tcataaatca tcgctttcgg gatcacaacc ttgccacctg ataccgcaaa
    18061 cggataggcg gtgttatccg ggttgttcgg gtcaaagaca aacaactgcg acgcagaaat
    18121 tgcaacctga cttacaggcc tgccttcact gtcttttccg gcgacaatcc cgatccccgc
    18181 agtgatacca tcaactcccg ctttttttga ccacattgca agaaaagctt caccgccttc
    18241 tttatccaat ttagtgatgc gcttgtcgac ctcattaagc gattcactgg ttgacgaatc
    18301 cagtgtgcta atgcgggttt caataccacc aatcgttctt gtcgtttctt ccctgagagt
    18361 ccccacaact tcggttgtct taattgcagc atcctttaca gcctgcccct gcgcgttttt
    18421 tatttcttta cgcagctcgg acacaaccgg cgactttgca gcctcatcgc ggatctggtc
    18481 aatgatggcc ttcacgccga tctgtgtttg tgcctgagtg cctttttcag cattccatgg
    18541 acctttcact cctgccgcgt taacaaaacg tatccagtaa aatcccgacc agccagggtc
    18601 aaccggatcg ccgtaaacct gccccggcgt cgtggcaacc agcactgcat cagcaaggtc
    18661 atcctccgta ccccgccaga tttcagtcag tgaatgtccg cgataattag gcatatccca
    18721 ttcaagaaga accgagccaa atcctcctgt cgccttaaaa ttcagcggtt ttgtgggaaa
    18781 atcaacagtc attaaagtac tgtcaatctc aatacccgga ttcagtgcat atgaggcacc
    18841 acccgatgtt cgacgccggg cgagtttaag acctaccagt tcttcacggg tcacaaacgc
    18901 gtgacgtccg tcaccacgct gcccggtgcc aatttccata ttctccacaa cagtggataa
    18961 atccttcccc gcccgccacg gttttttgct catatcggta actccgacat cgatgtactc
    19021 agggttattc gttccacctg cccgaatccg gataccatca cctgccagtt ttgcccggtt
    19081 gctgccggaa gtctcaccac acttccctta aacgtacccg gcgcaaaatg aatcacagga
    19141 acatcatcag ccataatggt gatccccacc cgctcaggcg ccggagattt caccctgata
    19201 caggaaaaag aggttctttc aggtaatgaa aaaatttttg aatgccacct tatcgtggag
    19261 ggcagagagc ccccggcaag cactgacatt ttgtctcctg tcaccacacg catcatatct
    19321 ttcgcgagat caacccatgc gcagtcaaac ggtgtactga gataacggat atccatgttc
    19381 accggactga atacaaacac atcctgctta ccatccggtt tcgtgtaaca ggcaatgtac
    19441 tcaccacgcc aggaataagc cacaatggac gccgggttaa actgactctg ccactgttca
    19501 ggtgaaataa tcttttccgt tgccagtgct gtattaccgt ttacatcaac agataccaga
    19561 ccgtttgtcc cggcatagag tacgaatccc tccatcgcca ccatacttct tcggctcagg
    19621 catgcctgca tggaaggaat tctggagcca gaaatggtgg aaggcgatac accactgaac
    19681 agataaggct ccccctttgt cgccaccacc agtgacgttc ccagcggaca gatagccaca
    19741 atatcttctg ccgtcgtgtg acgattcact tccggccatg catacggcag atacgcttcc
    19801 gaaaacatca cttcattacc ggcaaacccg gcggcaatac cattagccat caggcaaagg
    19861 cctgtcatat tctctggcgg cggcaggtaa tcccatgtag ccagagaagg tccaaggttt
    19921 ttccccggta ttttgtccgt gtaactgagc acggatgcat ccagttcagc cacaagtaaa
    19981 aaatccgctt ctcctccacc tgatgcagag cgataaatcc ggcggcgttt aatactggca
    20041 ttctgcaatg gcaccggaga cagcgtcagc tgtaccgcag tccccggagt acggagtgtt
    20101 acctccagag acgccggacc tggcggacct tcttcaccat aatctgagac aaaggtttcc
    20161 gtataaaacc gggtttcgtc atcattcggg ttatcgtcag aaacatcacc gccctgctga
    20221 acagtacaga caggtgctgt cgtcggcgcg gggatcccca gacgatacga tgatgtcggg
    20281 tgattcccgt ccccttttgt ggcaatggtc gcatccgtca ctttaggaaa acgcccgtca
    20341 gtgtagtaaa tacgcccgtg ggagtcctga gcgaccggac tgcggatcac atccaccaca
    20401 tccgtccatg caaaccagaa atcgtcacgg taatgaaaaa tggttttcgg cttaatcgcg
    20461 aatgttttct cagcctctga catctggtgt tcaggcgtga tcactccata gcgaaaatga
    20521 cagttttctg ccagtacagc agaatgatct ggcagcatag atgcaataac gcctggcatc
    20581 atcccgcgca tagttgttat atcgatataa ggcatggttg tctggtatcc gaaaatttga
    20641 atggcaggtg agtcactgaa ttgaagtttt aaaggcggca tacaattgcg cctgtgcaga
    20701 atacagattt ccagaaatct gaaaaattac tcttatcaga gaagagcgtc agaaacacga
    20761 tcggcatcac tgtttctgca aacttccctg tggcagtctt tattcgccat ataacgtagc
    20821 actaaaggtc ttcattaatt tttccattgt agttactcct tccttgtacc gggggttggt
    20881 catgcccccg ttttttattc cctctgctcc tttaatcgca ctgccaggcg ccacaacaca
    20941 catcaactgt ggctggtatg gaaacaccaa aaactctcac ttccggaaat ctgaaaaatt
    21001 atccatatca acaggatgcg ccggaaacat gatttccatc acatccaggc ctgcactttc
    21061 cgtgacagta tttattcgca tagtaaatac ttcacaagtg tataattgac tcagttcatg
    21121 ggggaggtga tggtccccca tctttttgtc tgttattcct gtgcccaacc aaccctgtat
    21181 gccagtatct catccgcact ggt
//
LOCUS       DAZGBC010000083        20831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGBC010000083 DAZGBC010000000
VERSION     DAZGBC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.083"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..1808)
                     /locus_tag="ACHK1J_002932"
     CDS             complement(81..1808)
                     /locus_tag="ACHK1J_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO7912175.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPQPENKESQQQQPSTPTEQQVLAA
                     QQAAIKEAEQSAAAAKALAQQEVQQYSDKQALQGRLQEAPTFARAAKAKATHIANPGT
                     ARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYF
                     PSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLV
                     FLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAE
                     INAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIE
                     LMLKKQRESGVTLSTFGVGDDNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQ
                     TLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLF
                     ELTLNGQKASIDKLRYAPDNKLAKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTIN
                     APSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGVDPQGYRAEFIRLIEL
                     ADGVTDISQ"
     gene            1996..2916
                     /locus_tag="ACHK1J_002933"
     CDS             1996..2916
                     /locus_tag="ACHK1J_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO7912176.1"
                     /translation="MIIFYQPWVNALPSTPRHASPEQLEKTVRYLTQTVHPRSADNID
                     NLNRSAEYIKEVFVSSGARVTSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASS
                     YENDQLTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPHFFRSDEMGS
                     AVHAASLEHPVKLMIALEMIGYYDSAPGSQNYPYPAMSWLYPDRGDFIAVVGRMQDIN
                     AVRQVKAALLSSQDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAIMITDTAFYRNKQY
                     HLPGDTADRLNYQKMAQVVNGVITLLYNSK"
     gene            3019..3522
                     /locus_tag="ACHK1J_002934"
     CDS             3019..3522
                     /locus_tag="ACHK1J_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO7912177.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRNELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(3589..5046)
                     /gene="nuoN"
                     /locus_tag="ACHK1J_002935"
     CDS             complement(3589..5046)
                     /gene="nuoN"
                     /locus_tag="ACHK1J_002935"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO7912178.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(5053..6582)
                     /gene="nuoM"
                     /locus_tag="ACHK1J_002936"
     CDS             complement(5053..6582)
                     /gene="nuoM"
                     /locus_tag="ACHK1J_002936"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO7912179.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(6746..8587)
                     /gene="nuoL"
                     /locus_tag="ACHK1J_002937"
     CDS             complement(6746..8587)
                     /gene="nuoL"
                     /locus_tag="ACHK1J_002937"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO7912180.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(8584..8886)
                     /gene="nuoK"
                     /locus_tag="ACHK1J_002938"
     CDS             complement(8584..8886)
                     /gene="nuoK"
                     /locus_tag="ACHK1J_002938"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO7912181.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(8883..9437)
                     /gene="nuoJ"
                     /locus_tag="ACHK1J_002939"
     CDS             complement(8883..9437)
                     /gene="nuoJ"
                     /locus_tag="ACHK1J_002939"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO7912182.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(9449..9991)
                     /gene="nuoI"
                     /locus_tag="ACHK1J_002940"
     CDS             complement(9449..9991)
                     /gene="nuoI"
                     /locus_tag="ACHK1J_002940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO7912183.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(10006..10983)
                     /gene="nuoH"
                     /locus_tag="ACHK1J_002941"
     CDS             complement(10006..10983)
                     /gene="nuoH"
                     /locus_tag="ACHK1J_002941"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO7912184.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(10980..13706)
                     /gene="nuoG"
                     /locus_tag="ACHK1J_002942"
     CDS             complement(10980..13706)
                     /gene="nuoG"
                     /locus_tag="ACHK1J_002942"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO7912185.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(13759..15096)
                     /gene="nuoF"
                     /locus_tag="ACHK1J_002943"
     CDS             complement(13759..15096)
                     /gene="nuoF"
                     /locus_tag="ACHK1J_002943"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO7912186.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(15093..15593)
                     /gene="nuoE"
                     /locus_tag="ACHK1J_002944"
     CDS             complement(15093..15593)
                     /gene="nuoE"
                     /locus_tag="ACHK1J_002944"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO7912187.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(15596..17398)
                     /gene="nuoC"
                     /locus_tag="ACHK1J_002945"
     CDS             complement(15596..17398)
                     /gene="nuoC"
                     /locus_tag="ACHK1J_002945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO7912188.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(17492..18154)
                     /gene="nuoB"
                     /locus_tag="ACHK1J_002946"
     CDS             complement(17492..18154)
                     /gene="nuoB"
                     /locus_tag="ACHK1J_002946"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO7912189.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(18170..18613)
                     /gene="nuoA"
                     /locus_tag="ACHK1J_002947"
     CDS             complement(18170..18613)
                     /gene="nuoA"
                     /locus_tag="ACHK1J_002947"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO7912190.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(19244..20182)
                     /gene="lrhA"
                     /locus_tag="ACHK1J_002948"
     CDS             complement(19244..20182)
                     /gene="lrhA"
                     /locus_tag="ACHK1J_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO7912191.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         5227 a         5697 c         5239 g         4668 t
ORIGIN      
        1 agtaagttaa catcattgtt aattaattcg accagtcagc aaatatgctg actggtcgaa
       61 catagtttgc taaaaagtca tcactgactg atgtcagtca caccatccgc cagttcaatc
      121 aggcgaataa attccgccct gtaaccctgt ggatctaccc cttttgcctg ctgagcccac
      181 tgtttgatct gctgccagga ggtattgttc aggtattcag aaccgcgtaa cttttgccca
      241 tatgcagcta ctgctgcgcg aaaacgcata tcttcagagg gcgcgtttat tgttggcccc
      301 agcgggaatt caactaactg actttctttt ccctgcgggt atttccagcg aatttttaac
      361 caggccagtt ctttcgtttt gtccgatttc gctaatttgt tatccggggc atagcgtaac
      421 ttatcaattg atgctttttg cccgttcagc gttaattcaa ataacaacgt tatatgtttg
      481 cctgcgccaa tatcccccgc atcaacgttg tcgttattaa aatcttccgc ccgaagttgg
      541 cgcttttcat aaccaatctg acggtattcc gttacccacg cggggttaaa ctcaatttgc
      601 gctttgacat cttttgctac ggtaatcaac gtctgccgca tttcactgtt caatactttc
      661 tgcgcttcag agagggtatc aatgtagctg tagttaccgt tgccaacatc ggcaattcgc
      721 accatcattg cctcgttgta attgtcatcc cccacgccaa acgtcgacag agtaacaccg
      781 gactcccgct gttttttgag cattaattca atcgattttg gatcgtcaat accaacgtta
      841 aagtcaccgt cagtggccaa taaaatgcga ttgataccgc ccttaataaa ccccttcgcc
      901 gcctgctgat aagccagttc cagcccggca ccgccattgg tactgccttc ggcatccagc
      961 gaatcaattg cggcattaat ttccgcctta tgactcccgg agatagaagg caatgcaata
     1021 cgggagtcgc cagcgtaggt cacgatggca atgttatcct gctcacgaag ttctttgacc
     1081 aataatttca acgaagactg gataagtggc aaacgttcat cagaaatcat tgaaccagaa
     1141 gtgtcgataa gaaagaccag attagaagct ggtaactctt cacttttgcg atctttcgcc
     1201 aggatatcaa ctttcagcaa tgttcgctgt tcattccatg gtgcaggtgc caattcgtag
     1261 cgcatagcga aaggtattgg cttagaggcc ggaatagatt gtttgtcttt gatatcccaa
     1321 tcagacggga aataattgac tatctcctcc acccgcacag cgtctggcgg aggtaacagc
     1381 ccttgattga ggaaacgcct tacattcgca taactgccag tgtcaacgtc aagactaaac
     1441 gtcgccaacg gattttgcgc tacctgctta accggattat catcgaactg ctggtagcga
     1501 gcggttcctg gatttgcgat atgtgtagct tttgctttag ccgctcttgc aaatgttggc
     1561 gcttcctgca atcgcccctg taaagcctgt ttgtctgaat attgttgcac ttcttgctgg
     1621 gccaaggctt tcgcggcggc ggcgctttgc tcagcctctt ttattgcagc ttgctgcgcg
     1681 gcaagcactt gctgctctgt gggagtactg ggttgttgtt gctgactttc cttattctca
     1741 ggttgcggcc cacatcctga caaaatcaaa ctactcataa gcaacatgat tatattttta
     1801 tttcgcatat aaaatccttt taaataaact caggcaccag acataaaata tactctttgt
     1861 ataatcctgc taatcttaat aaaatacaat atcgaaaccc atagcaaata atgattcaac
     1921 aacgcaaccc attaataatt tgcatataaa aaaataatct ttgcttttat tatattattt
     1981 gtgtttttac tccctatgat tattttttac caaccctggg tcaatgcatt accgtcaacg
     2041 ccccgacacg caagtcctga gcaattagaa aaaacagttc gttatcttac acaaactgtg
     2101 catccacgta gtgccgacaa tattgataat ctgaataggt ccgcagagta tataaaagag
     2161 gtctttgtca gtagcggtgc cagagttacc tcgcaggatg tccccattac gggtggcccc
     2221 tacaaaaaca ttgttgctga ttatggtcct gccgatggac cgctgattat tattggtgcg
     2281 cattatgact ctgccagcag ttatgaaaac gatcaattga cctatacgcc gggcgcggat
     2341 gataacgcca gcggtgtggc aggattactc gaactggcac gtttgttaca tcagcaagta
     2401 ccgaaaacag gcgtgcagct ggtcgcctat gcgtcggaag aaccgcactt ctttcgtagc
     2461 gatgaaatgg ggagcgcggt gcatgcagct tcgcttgagc atccagtgaa attaatgata
     2521 gcactggaga tgattggcta ttacgactct gcgcctggaa gccagaatta cccttacccg
     2581 gcaatgtcct ggctttatcc cgatcgggga gactttattg ccgtggtcgg cagaatgcag
     2641 gatatcaacg ccgttcgtca ggtaaaagcg gcattgttgt catctcagga tttatctgtt
     2701 tattctatga atgccccagg gtttattccc ggtattgatt tctctgatca cctgaattat
     2761 tggcaacacg atattcccgc cataatgatt actgacaccg ctttttatcg taataaacaa
     2821 taccacttgc ccggtgatac cgcagacaga ttgaattatc agaaaatggc tcaggtagtg
     2881 aatggtgtta taactttgtt atacaacagt aaataataaa aatttctcga ggtgggtcca
     2941 gaaaaagcga catgcacatt ttattttccc tcatctgttt taagatgcga aacatgaaat
     3001 tcaacggagg aaaaataaat gggaatgatt ggctattttg cagaaattga ctcagaaaag
     3061 attaaccaac tgcttgagtc tactgaaaaa cctttgatgg ataatataca tgacacgctt
     3121 tcaggattac gccgactgga tattgataaa cgctgggatt ttttacactt tgggctgaca
     3181 ggcacctctg cgttcgatcc agcgaagaat gatccgctga gtcgtgccgt gcttggtgaa
     3241 cacagcctcg aagatggtat agatggtttc ctgggattga catggaatca ggagttagcc
     3301 gcgacaatcg accgactgga atcactggat cgaaatgaac tgcgtaaaca gttttccatt
     3361 aagcgactca atgaaatgga gatttatccg ggagtgacct tttcagaaga gttagagggg
     3421 caactctttg cttcgatcat gcttgatatg gaaaagctga ttagcgcgta tcgacgtatg
     3481 ctgcgacaag ggaatcatgc gctgacggtt attgttgggt gatggtgtca gggcaccaaa
     3541 aaaaaccgcc gaacatgtcg gcggctttct gacttacaaa ctaacagatt acatcagtgg
     3601 cattgctaac cgcacaatgc taatcagcgg ttgtggccat acacccagca ccagtaccaa
     3661 cagtgcggag atcagcacca cgataccgcc cgcgctgtac tgccagtttg acggtgcatc
     3721 gcgacccggt tgttccggtg cgtgcaggta caggctcacc gccacgcgca ggtagtagta
     3781 gaggccgatt gccgaaccga caaccacggc acccaccagc caccacaagt gtgcctggac
     3841 accgaccgcc agcacgtaga acttaccgat aaagcccagc gtcatcggga taccggccag
     3901 agacagcatc atcaccgtca tcaccgccgc gaggatcgga cgatgccaga acagaccgcg
     3961 gtaagagaac agggaatcag catccgggcc acgatacggg ctggacatca ggctgaccac
     4021 gccgaacgcg ccgaggctgc tgaacagata accggccagg taaaccccta ccgcttccat
     4081 cgacatctcg ccggtttgca gcgcaatcag cgccaccagc agatagccga ggtgagagat
     4141 agatgagtaa ccgagcagac gtttgatgtt ggtctggctc agcgccatca ggttaccgaa
     4201 gatgatggag gcaaaggcga taatcgccag caccacccga atcgcttcgc tgtcacccac
     4261 cggtgcgtag aggaacagac gcatcaccac accgaagata gcgattttgc tcgccgtcgc
     4321 caggaaagtg gaaaccggcg caggcgcgcc ctggtatacg tctggcgtcc acaggtggaa
     4381 cggcaccaga gagagtttga agccgaggcc aacaatcatc aggccgaaac ctgccagcaa
     4441 cagcggctcg ttgagcatac cgtcgccgag gtttttaccc agcgcgacaa acgacaggtc
     4501 gccagactgc gcatacacca gcgccatacc gaacagcagg aaagaagacg ctgcggcaga
     4561 aaggatggtg tatttgatac tggcttccag cgaacgtttc tggcggaaag cgtaaccgac
     4621 caggccaaac agcggcaaag agatcagttc aataccgagg aacagagacg ccagatggtt
     4681 tgcattcgcc agcaggatcc cgcccagcgc ggcaattaac accaacaggt agaactcatc
     4741 cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa gtacaggtgg cgaggctcgc
     4801 caacaatacc agcccggtgt aaagcatggc gaaaccatca acgcgcatca gcggcgtaac
     4861 gtccatagcg cccgcctggc caacaaacca gagcgaaacc agcgccgcgt taagcccaat
     4921 aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac gcaatggaga gcatcacaac
     4981 caccaccgtc aagccgacga tcagcaacgg tagcagtgcg atcaggtttt gtggagttat
     5041 tgtcatggcg atttacggcc ttgtagtagt aacggaatta acaaaccact gctggatatt
     5101 gccaatcgcg gagtgcgagg tatccagaat cggctgcgga tagaagccca gcagtaccag
     5161 cagcaccacc agcaacagga tcataaacag ctcacgcagc gacatccctg gcagttcctg
     5221 gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga tgtaacatcg ccagcgaata
     5281 aacagatgca aagaccagcc caaaggtaga gataacggta atcaccggga caacctggaa
     5341 gctgccgaac agaatcataa attcgccgac gaagttaccg gtgccaggca tcccaagcgt
     5401 tgccaccgca aagaacagcg acagtgctgg cagccatttc atcttgctcc acagaccgcc
     5461 catcatgcgc atgtcgcggg tatggatacg ttcataaagc tgaccgcaga gaataaacag
     5521 acccgccgct gacaagccgt gcgcaatcat ctggattacc gcgccctggt aggccaactg
     5581 gctgccggtg tagatagcaa tcagtacgaa gcccatgtgg gaaaccgagg tgtaggcgat
     5641 cagacgtttg atatcggtct gggcgaaggc catccacgca ccgtagaaga tgccgataac
     5701 acccagccac atagcgattg gcgcgaactc tgccgacgca tttgggaaca gcggcaggga
     5761 gaaacgcagc aaaccgtaag cggcagtttt cagcaagatc cccgcgaggt caacggaacc
     5821 ggcggtcgga gcctgggagt gcgcatccgg cagccagcca tgcagcggaa ccaccggcat
     5881 tttgactgcg aaggcgatga agaagcccag catcaacaga tattccacac cactggacat
     5941 tggcgtattc aacagctctt catagttgaa ggtccagacg ccggtcgcat tgtagtgaac
     6001 aaaaaccagc gccaggatgg cgatcaacat caccagacca ctcgcctggg tgtaaatgaa
     6061 gaacttggtt gccgccgtga tacgcgtttt accgtcagag gctttatgcc cccacagtgc
     6121 gatcaggaag tacatcggca ccagcatcat ttcccagaag aaaaagaaca ggaacatgtc
     6181 gatggcaagg aacacgccga taacgccgcc caggatccac atcaggttga ggtggaagaa
     6241 gccctgatat ttttcgatct ctttccacga acagagtacc gccagcacac cgagcagacc
     6301 ggtcagcacg accattagca gcgacagccc gtcaatggcg agatggatag agataccaaa
     6361 acgcgggatc cacggcatgt cgaattcaga ctgccactgc ggaattccgg cggattgcgt
     6421 cagtgaataa ccgccctgca accacagttg cagcgacagc gccagcgtca atcccatggt
     6481 gatcagcgcg atccagcgcg gcaccttgac gccaaagcgt tcggtctgcc agcacaggaa
     6541 gccgccgata aagggaatta atattagcca gggtagtaac atggcgatct ttattccttg
     6601 taaaagtccc gtcaggaccg gattttcaac gaattctcac gacagaaaat tctcttcgga
     6661 ctgggggtag tgtcggatgc accgcgaagg aggcttatcc gacctacgtt cttatatgcc
     6721 caggggcaat cccacaatcc ttaactcaac gcagtaccat caacagtgcc agcaccacga
     6781 ccgcaccgat gctcatggat gccacatacc agcgcagata accgttctcg cttaacagca
     6841 ggcctttacc tgcaaagcgg gaaagaaccg ccggaatgtt catcattgag ttcagcggat
     6901 cgcgtttcag caaccaggca atacccagga acggcttgac gaacactttg tcgtacagcc
     6961 agtcaaagcc ccaggcgttg taccaccagg tgcccagcag acggcccggc gcactgttgg
     7021 cgatggaggt caccagagta cgtttaccca gccacagcca ggctgccagc agaatgccga
     7081 ccaccgcgac cacgccagag gtaatttcca gggtcaacat gctgccgtgc gccagttccg
     7141 tcgtttgcgg aagcacgccc tgcagcggcg gtacaatcag tgcgccaacg aaggtggaaa
     7201 ggatcagcag cacaatcagc ggcaggctgt gagttacccc tttcacggcg tgagcgtgaa
     7261 tttgttcttt tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc gaggtcataa
     7321 acgcaccgac cagacctgcc accatcagat tgatatgacc attcgccatc gcacccgcga
     7381 ggatctcatc cttactgaag aagcccgcag tgaccagcgg cagtgccgac agtgctgcgc
     7441 cgcccaccag gaagcagaga taaaccagcg gaatagattt acgcagaccg cccatcttga
     7501 agatgttctg ttcgtgatgg caggccagaa tgacggaacc ggatgccagg aacagcagcg
     7561 ctttaaagaa cgcgtgggtc atcaagtgaa aaatcgccgc atcccatgcc tgcacgccaa
     7621 gcgcgaggaa catgtagcca atctggctca tggtagagta agcgagaaca cgtttgatgt
     7681 cggtctgtac cagcgcggca aaaccggcca gcagcagcgt aaccgccccg acaataccca
     7741 ccagatgcag aacttccggc gtcatcagga acaggccgtg ggtacgggcg atcaggtaga
     7801 cacccgcggt taccatcgtt gcggcgtgga tcagcgcgga gacaggcgtc gggcccgcca
     7861 tcgcgtcggc aagccatgtc tgcaacggca actgcgcaga tttaccgacc gcaccgccca
     7921 gcagcatcag cgtcgcccac atcagcatat tattgccgtc agcaaagtgc gctggtgcca
     7981 gttccaccat ttcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg aacagcgcga
     8041 aagcgaggaa cacgtcaccc acacgggtca cgacgaacgc tttcattgcc gctgcgccat
     8101 tcttcggatc ggtgtaatag aaaccgatca gcagatagga gcacaggccc acgccttccc
     8161 agccgaggta catcagcagc aggttgtcgg caagcaccag aactaccatg ctggcgatga
     8221 acaggttggt gtaggcgaag aagcgagagt agccctcttc accgcgcatg taccaggagg
     8281 cgtacatgtg gatcaggaaa cccacaccag tgaccaccga gagcatggtc agcgacaggc
     8341 cgtccagcac cagattgaaa ccgatgttaa agtcgcctac cgacatccac gtccacagcg
     8401 gctggctgta agcctgctcg ccgttagcga agaaatcaac gccgataaaa gcggttacca
     8461 gcgccgccag gcccacagag ccaacgccga cgatcgccga aacgttttca gaccagcgcc
     8521 cacgggagaa cgccagcagg acgaagccaa tcaatggcaa aataatggtt aaggcaagca
     8581 tgttcatccg cgcatctcac ttactgaatc gatgttcagg ttctggcgac gacggtgaag
     8641 ttgcagcagc agcgcaaggc cgatactcgc ttctgccgcc gcgaggctga tggcgagaat
     8701 gtacatcacc tgaccgtcgg tctgacccca gtagcttccg gcgaccacaa aggccagcgc
     8761 ggaggcgtta atcatgattt ccagaccaat cagcataaac agcagattgc gacggataac
     8821 cagaccggtt aagccaagaa cgaataagat tgccgcgagg atcagtccat gttgtaaggg
     8881 gatcatgcgt gctcctccgt ttttcttttc gcgctgtcgt ctttacgatt gctcagcact
     8941 tcacccgcac gctcttcacg accgacgtgg aaggccacaa ccagacccgc gagcagcagc
     9001 atagaagcca gttccaccgc cagtacgtaa ggcccgaaca gcgtaatacc cactgcttta
     9061 gcactgattg gcgtaccgtc gataccttga tcgttaacac cgagaatggc gtaaacaatc
     9121 accaccaaca tgatggctga caaaattgcc ggaccaatcc acacctgcgg tttcagccac
     9181 tggcgttctt gttcgatttc tgaaccgccc aggttgagca tcatcaccac gaacacgaac
     9241 agcaccataa tggcacccgc gtagacgata atttccagcg caccggcgaa gtaagcgcct
     9301 agtgagaaga acaccccgga aatcgccagc agcgaaataa tcaggtacag cagtgcgtgt
     9361 accggattgg tatgggtgat cactcgcaag gtcgcaagta tggctatcag gccacagata
     9421 taaaaagcga actccattgc ccctctcctt acggtaacag gctcttgacg tcgataggct
     9481 tggcttcgtt ctctgcttcg cccttatctt tgccgtcgat tgccatacct gccatccggt
     9541 agaagttata ttccgggtat ttgcccggac cggagatcag cagatcctct ttctcgtaaa
     9601 ccagatcctg gcgcttgtat tcccccattt cgaaatccgg ggttaactga atcgccgtgg
     9661 tcggacaggc ttcttcgcac aaaccacaga aaatgcagcg tgagaagttg atgcggaaaa
     9721 attccggata ccagcgaccg tctttggttt ctgctttttg cagcgagata cagccgaccg
     9781 ggcaggctac cgcgcagagg ttacaggcta cgcaacgctc ttcgccgtcc gggtcgcggg
     9841 tcagaacgat acgaccacga taacggggcg gcagatagac cggctcttcc gggtacattc
     9901 gcgtttcgcg tttggcgaac gcgtgcaggc cgatcatcca gatactacga acctgggtgc
     9961 cgaaacctac taacaattct tttaaggtca tggtcttatt gccccttatt gcgcctgcca
    10021 gagaatgaca gccgccgtta ccagcaagtt gatcagcgtc agcggcaggc agattttcca
    10081 gccgaaggac attacctggt cataacgcgg acgcggtaac gacgcacgaa tcaaaatgaa
    10141 catcatcata aagaacgcgg ttttcagcgc gaaccagatg aatggcggta acaacgggcc
    10201 ttgccagcca ccgaagaaca gcgtcaccat caatgcagag atggtcacaa tcccgatgta
    10261 ctcacccacg aagaacagac cgaacttcat gccggaatat tcaatgtggt aaccatccgc
    10321 cagttcctgc tcggcttccg gctggtcaaa cgggtgacgg tgacacaccg ccacgcccgc
    10381 gatggcaaag gtaataaaac caaagaattg cgggatgacg ttccacacat gcgcctggct
    10441 gttgacgatg tcggtcatgt tgaatgaacc ggcttgcgcc accacgccca tcagggaaag
    10501 cccgaggaac acttcgtagc tcagggtctg cgcagaagca cgcatcgcac ccagcagcga
    10561 gtatttgttg ttacttgacc agcccgcaaa cagcaccgcg taaaccgcca aaccggccat
    10621 catcaggaag aacaaaatcc cgatgttcag gtcggcaacc acccaacccg gactgactgg
    10681 cacaatcgca aaggccagca gcagtgaggt aaaggcaatc atcggtgcca gggtaaagat
    10741 gacgcgatcc gagaatttcg ggatccagtc ttctttaaag aacattttga tcatatccgc
    10801 gaccagctgg agcgaaccgc cccagccaac acggttaggt ccgtaacggt tctggaacag
    10861 acccagcagg cgacgttcgc caaagctcat gaatgccccg caggtgacaa ccaccagcag
    10921 gatcaccacc gctttgagga tggtcagcag gatctcaatc agttccggtg atatccaact
    10981 cattgttgtg cctccttgag atcctcaaga tgcgcgccag ccagcaccgg agcaatgccg
    11041 gacataccca tcggcaagcc cacctgccct gccgtcagcc cttcagcgat ttcaaccggc
    11101 agcgtgaccg tgttgccatc gtaactaaag gagacgcgtg tacctgcgtt cacacccaac
    11161 ttcgcggcat ccgctgggtt gagtttgatg tacggctgcg gcatacggct ctggaagacc
    11221 ggagcacgct gtgacaattc atcactgcca aacaggtggt aatacggcgc aatacgccat
    11281 ttcccgtcct gcggctggaa gcgtgccggt acgctggtga agtaatccag accattctcg
    11341 ctggtttcaa acagacgcac gcccggatcg ccaaagcgca gtttgccgcc cacttcgtcc
    11401 tggaatttgt tccacgcctg cggggagttc cagcctggcg cccaggcaaa cggcacttgc
    11461 gaacggtgcg cggtcggctg gttgttacct tccatcgaga aggtgaacat ggtgtcaata
    11521 tcctgcggct gacgcggctc atgaacgcta atattggcgc gcatggcggt acgaccgctg
    11581 taacggtgcg gttcacgggc cagtttctgc ccacgaatac ggaatgtcgc atccggcgca
    11641 gcatctttga tacctgccag ttccgggatt ttcgccacaa cagcgtcaat cacatggtcg
    11701 agctgcgtcc agtccacttc acggctcaac agggtactgt gcagcgagtg taaccagcgc
    11761 cagctttcca gcatgacagt tttgctgtcg taataagccg gatcgtaaac ctggaagaaa
    11821 cgttgggcgc gaccttcatt attaattacc gtaccgtcgc tttcagcaaa gctggcagcg
    11881 gaaagtacca gatgggcgtt ttccatgatc gctgtgcgtt gatggtcaac caccatcacc
    11941 agcggcgctt tagccagcgc agcattcacg cgggtagcag aagcgtgacg atgcagatcg
    12001 ttttccagca ccaccaccgc gtcggcgcgt ccggtttcca gttcggttaa cgcttcttca
    12061 agcgatccac cgcccataat gcccagcccc atgctgttga cggaacgggc aatcatggtg
    12121 ataccgacgt cagcgccgcg acctttcagg gctttcgcga cgttagccgc agcctgaatt
    12181 acttcagcgc taccggcgtt cgtcccggag ataatcaacg gtttcttcgc acccgccagt
    12241 gcctgcacga tgacgtcgat tttgctttgc agttcaggtt caataccgtc aaccgctggc
    12301 gcggagttat ccagcgcatg ggcgatggca aaacctaaac gcgcctgatc ttcaaccggt
    12361 gcgcggtaag tccacgccgc gatatcatcc agacgggtgt catcaacgtt ggtaacaaac
    12421 agcggatgct tcgcacgttg accgatgttg aggattgccg caatctgcca gtcagccact
    12481 ttctgtgctg ccgccatttc gcgcgcttta cctttcaccg cctgacgcac tgccagcgcg
    12541 acgcgcgcgc cggtctgggt aacgtcttcg cccagcacca gtaccgcatc gtaagattcg
    12601 atttcgcgca gagcaggagt ataaatgccg ccttcacgca gtactttcag cgccagttgc
    12661 agacgttcct gctcaccgtg agcgataccg gtgtagaagt tttcttcgcc caccagctca
    12721 cgcagcgcaa agttactttc cacgctggcg cgcggagaac caatgccgat cactttcttc
    12781 gactgacgca gaatatctgc tgcgccctgc attgcctgtt cggcgttgag ggtaatgaaa
    12841 tcatcgccac gacgctgtac tggctgacgc ggacgatctt tcaggttgac gtaaccgtaa
    12901 ccgaaacgac cacggtcgca gaggaagtag tggtttaccg taccgttgta acggttttcg
    12961 atacgacgca gttcgccata acgctcgccg gggctgatgt tacagccgat ggaacattgc
    13021 tggcagatgc tcggcgcaaa ctgcatatcc catttacggt tgtaacgctc ggagtgcgtt
    13081 ttgtcggtaa atacgccggt cgggcaaatt tcgaccaggt taccggaaaa ttcgctttcc
    13141 agcgtgccgt cttccgggcg accgaagtag acgttgtcgt gcgcgccata aacgcccaga
    13201 tctgtaccgt cagcgtaatc tttgtagtaa cgcacgcagc ggtagcaggc gatgcagcgg
    13261 ttcatttcgt gagagatgaa cggccccaaa tcctgattac ggtgggtacg tttggtgaaa
    13321 cggtagcgac ggaagctgtg tccggtcatc acggtcatat cctgaagatg gcagttaccg
    13381 ccttcttcac ataccggaca gtcgtgcggg tggttggtca ttaaccactc gaccacactt
    13441 tcacggaact gtttcgcttc ttcgtcgtca atggaaataa aggtgccatc ggaagccggt
    13501 gtcatacagg acatcaccag gcgaccacgc gtgtcttccg cgttttggta ttgcttcacc
    13561 gcacactggc ggcaagcacc gacgcttccc agcgccggat gccagcaaaa gtaaggaata
    13621 tcaaggccca gagacagaca agcttccagc aggttgtccg ctccgttgac ctcgtattct
    13681 ttgccgtcta catgaattgt agccattagc atgcttccag ttttctcagt cagagactga
    13741 gcgttaatcg aaattcggtt accagcgctc tttcagcagg ttcggctgaa tcccattaat
    13801 caaatgggta ttgctgaacg gctgtttgat ccccgcctca aattcttcgc ggaaatattt
    13861 gatggcgctc tgtaacggct ccactgcacc aggtgcgtgg gcacagaaag ttttacccgg
    13921 gcctaagaat cgacacagtt gctcaagtgt ttcgatatcg cccggctgac cttcgccacg
    13981 ctccagcgca cgcagaattt tcacgctcca cggcagaccg tcgcggcacg gcgtacacca
    14041 gccgcaggac tcacgggcga aaaactcttc caggttacgc accagcgaca ccatgttgat
    14101 ctcatggtca accgccatcg ccagcgccgt acccagacgg cttcccgctt taccgatact
    14161 ttcgaattcc atcggcagat caaggtgcgc ttcggtcagg aagtcagtcc ccgcgccgcc
    14221 tggctgccag gctttaaatt tcagaccatc acgcatacca ccggcgtaat cttcgaggat
    14281 ctcgcgtgcg gtggtgccga acggcagttc ccacagtccc ggatttttca cccgaccgga
    14341 gaagcccatc agcttggtgc cagcatcttt acttttcgag atgttctgat accactccac
    14401 gccgttagcg aggatcgccg gaacgttaca cagggtttcg acgttgttga cacaggtcgg
    14461 tttgccccat acgccggagg ttgccgggaa ggggggtttc gagcgtgggt tagcacgacg
    14521 cccttccagg gagttgatta acgctgtttc ttccccgcag atgtagcgcc ctgccccggt
    14581 atggacgaac agttcgaagt cgaaacctgt tcccataatg tttttgccaa gcaagcccgc
    14641 ttcagtggct tcggcaatgg cgcggcgcag atttactgcc gcttcgatat attcgccgcg
    14701 caggaagatg tagccacggt aagctttcag cgcaaacgcg gagatgagca taccttccac
    14761 cagcaggtgc ggcagttgct ccatcaacag gcggtcttta taggtgcccg gctccatttc
    14821 atcggcgtta cacagcaggt aacggatgtt catggattcg tctttcggca tcaggctcca
    14881 cttcaggcca gtcgaaaagc ccgcgccgcc gcgccctttc agaccagcgt cttttacctg
    14941 attaacgatt tcgtccggag acagcccggt cagcgcctta cgcgcccctt cgtaaccgtt
    15001 tttgctgcgg tattcgtcca gccacactgg ctgtttgtca tcgcgcagac gccaggtcag
    15061 cggatgcgtt tcgggagtac ggataatgtt tttcatttat accgctccag cagttcaggg
    15121 atcgcttccg gggtcagatg cgcgtgagtg tcctcatcga tcatcatgtt tggcccttta
    15181 tcacagttcc ccaggcagca agttggcagc agcgtaaagc ggccatcaaa tgtcgtttgc
    15241 cctggtttga tgttcagctt tttctcgagc gccgcctgaa taccctgata cccgttgata
    15301 tgacagacca cgctgtcgca ataacggatc acatggcgac caaccggctg acggaagatc
    15361 tgactgtaga acgttgccac accttcgacg tcgcttgccg gaatacccag cacatcggcg
    15421 atcgcgtgga tcgcaccatc cggcacccag ccacgctgct tctgaacgat tttcagcgct
    15481 tcaatggacg ccgcacgcgg gtcttcgtag tggtgcatct cgtgctcaat cgcttcacgc
    15541 tctgccgcac tcagctcaaa agcctcggtt tgtggttgtt gattctcgtg cataattagc
    15601 ggtccacatc tgacataaca aaatcgatac tgcccagata aacaatcagg tcagacacca
    15661 ggctgccgcg gatcgccgcc ggaatttgct gcaaatgcgc ataactcggc gtgcggatac
    15721 gagtgcgata actcatggtg ctaccgtcgc tggtcaggta gtaactgttg atccccttgg
    15781 tcgcctcaat catctggaaa gattcattgg caggcatcac cggcccccac gacacttgca
    15841 ggaagtgggt gatcagggtt tcgatatgtt gcagcgtgcg ctctttcggc ggtggcgtgg
    15901 tcagcgggtg atccgctttg aacgggcctt ccggcatgtt gttgaggcac tgctcaagaa
    15961 tgcgcagact ctggcgcagc tcttccactt tcagcattac gcgggtgtag cagtcagaaa
    16021 caccaccacc aaccgggatt tcaaagtcga agttttcata gccagaataa ggacgcgcct
    16081 tacgcacgtc gaagtcgatc ccggtagcac gcaggcccgc gccagtggtg ccccactcca
    16141 gcgcctcttt cgcgccatag gcggcaacgc cctgggaacg acctttcaga atggtatttt
    16201 gcagcgccgc tttctcgtaa gacgccagac gtttcggcat ccagtcgagg aactcacgca
    16261 gcaggcgatc ccagccacgc ggcaggtcat gcgctacacc gccaatacgg aaccacgccg
    16321 ggtgcatacg gaaacccgtg attgcttcca ccagatcgta aattttctga cgatcggtaa
    16381 aggcgaagaa cacgggcgtc attgcgccga cgtcctgaat aaaggtcgag atatacagca
    16441 ggtgactgtt gatgcggaac agttcggaga gcattacgcg aatgacgtta acgcgatccg
    16501 gcacggtgat cccggccagt ttctctaccg ccagcacgta aggcatttcg ttaacgcagc
    16561 cgccgaggta ttcgatacgg tcggtatacg gaatgtagct gtgccaggac tggcgttcgc
    16621 ccattttctc cgcaccacgg tggtggtagc cgatgtctgg tacgcagtcg acaatctctt
    16681 cgccgtcgag ctgcaaaacg atacggaaag caccgtgcgc cgacgggtgg ttcggaccga
    16741 ggttgaggaa catgaagtcc tcgttttcgg tgccgcgctt catcccccac tcttccggtt
    16801 tgaaggtcag ggcttccatc tccagatcct gtttggcttt ggtcagctca aacggcgaga
    16861 attcggtagc gcgcgccggg taatctttac gcagcgggtg acctttccag gtttgcggca
    16921 tcatgatgcg gcgcaggttc gggtgaccgt cgaatgtaat gccaaacaga tcccaggttt
    16981 cacgctcata ccagttagcg ttcgggaaca gtttggtgaa ggtcggtacg tgcaggtcgt
    17041 tttctgccag cgccaccttc agcatgatgt cgcggttacg atcgatagaa atcagatggt
    17101 agaaaacgga aaaatccgcg gcaggtaacc cttcgcggtg tgtgcgcaga cgttcgtcca
    17161 tgccgtgtaa gtcaaacagc atgacgtaag gtttcggcag tttctttaag aaatcgccaa
    17221 cttccagtaa ttgttcacgc ttgatccaca caacgggaac cccggtgcga gtcgcctgaa
    17281 cagtaaaggc atccggccca aaacggttgc gcagttcgcc aattaccgga tcatcaagat
    17341 gatcgcgggt ctgccaggcg ggttcttgcg cggttaagtc ggtcatattg ttcaccattg
    17401 caaatggtcc gtggtgactg tcggcgcagt aagcttcgcg aaatagattt aggaataagc
    17461 gaagaaaaat ccctttgccg acaggcgcaa attaaatctc gtcaggtgta cgcaggttgg
    17521 ttacggcaat gcgttcaccg cgcttgcgtt cgcgctctga ttgcatattg gcgcgataaa
    17581 cgccctgatc gccaaccacc caggagagcg gacgacgttc tttgccgata gattcctgca
    17641 acagcatcag tgcctgcatg tacgcttcag gacgcggcgg gcagcccggg atatacacat
    17701 caaccgggat gaatttatcg acgccctgca caacggaata aatatcgtac ataccaccag
    17761 agttggcaca ggcacccatt gagataaccc attttggttc cagcatctgg tcatacagac
    17821 gctgaataac cggtgccatt ttggtaaagc aggttcctgc aaccaccatc aggtcagcct
    17881 gacgcggcga agcacgcaat acttctgcgc caaaacgcgc cacgtcatgc accgcggtaa
    17941 acgaagtcac catctcaacg taacagcagg aaagaccgaa gttatacggc caaattgagt
    18001 ttttacgacc ccagttaacc atgtcattga gcttgcccat aaacacgttt ttgttaactt
    18061 cttgctccag agggtcggtt acgatctcct gcttttgcag ggggtaacgg tcgttctcac
    18121 cgttgggatc tatgcgggtg agcgtataat ccatcttaat gcctcgcggt tagcgttgac
    18181 gattagcgat actgttcgtt tccgggttca tacgctcgcg gcgtgaacgc gcgggcgtcc
    18241 agtccagcgc gccaatacgc accagataaa ccagacctgc cagtaacaca aaaataaaaa
    18301 ttgcagcttc cacaaagcct acccagccgc tttcgcggat agaggttgac catgcgaaca
    18361 gatacagcgc ttcaacgtcg aagataacga agaacatggc caccagataa aacttggcgg
    18421 acaggcgtaa gcgggcggag ccgaccgagt cgataccgga ttcaaacggc acgtttttcg
    18481 acctcgcgcg tgcgcgaccg cctaaaaacc aaccgcctac cagcatcagg caacacaggc
    18541 caatggcaac gataagaaag atagcgaatg cccagtgatg agcgatgact tcagtggatg
    18601 ttgacatact cattgcttac tcatcaaaag tagcgccaga ttcactgctc ttcacggcag
    18661 atggacgcca catcgattca tggggaggaa taaaaaaaac cttacaatca ctgtagaaat
    18721 tcttttatac agctaattga tgtggtcttt tactcctttc tataaccttt tgtcaacttt
    18781 aacaaaagct tcttcacatt agtttacata atatcaacac cattagcatt taatgccctt
    18841 tcaccccaga tccttgacga ctccaggata attagatgtt gttgaatcgt gtccgttgtg
    18901 aagcaatgga aaaaatacgg gtctattttg acaggaattt gtgtcgattc ctccccccaa
    18961 aagagagtat tttcttgatc tgtgacacgc ttttgtcatt ccataacaaa aacgcagcaa
    19021 caaatttacg tattttttaa catcattgta gcaggtgatt tttttcaggc gattatttgt
    19081 gcgtacgggg cgtgaatctc tggtgattga aaaatgaaca gttttgtacg ttctgcacga
    19141 tgtgaagaag gttctttact aaaaaaaagc cgctggggtt taaaacaccc ccagcggctc
    19201 gttttttaca ctattgtctc aggaattatc tatggtccgt cgattactcg atatcccttt
    19261 caatcaacaa tgaatcatcc ccttccggag cagatattgg gctgtattgc cacggattat
    19321 ggtagctctc catcgcctga tagatcacct gggccagctc attattactg gacgggtcat
    19381 agcacagcaa atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac
    19441 gcaagtccgg actcatcatc tcgaccggcc ttgccgtcac gccaagaccg gctttcactg
    19501 ccgcacgaac ggccggaagc gtcgaggcga cataagccag tcgccaggga atatctgctt
    19561 tattaagcgt cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag
    19621 ggatcggctc acctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg
    19681 tacgcaggtt caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt
    19741 cctgggattc cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta
    19801 atttcggata aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt
    19861 catctgaagc accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg
    19921 cctcatcatt aaaacgcagg attttcctgg cgtaaccaag aagttgaatg ccatgttcag
    19981 ttaacagttt gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac
    20041 gctgcatttg ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa
    20101 aagtgttcag atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta
    20161 tcggacgatt tgcacttatc atatattatc acttactggc ggctcatact gagctggtta
    20221 atgctgtgca cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc
    20281 cggaacccgg acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt
    20341 tccgttataa tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa
    20401 gttactcact cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc
    20461 ttgttccact ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg
    20521 aggcggcatc aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt
    20581 atttataagg gtcattgcga attatctgat gcaaagttat gttatgttag gcaaagtaat
    20641 cttctattat taataagcac atcaaaacct ttttgaatat taaataataa ttaattagca
    20701 tcatcctcat tcattaattc cgcttaacaa tagtttcaca ccttgccccc attgcgacac
    20761 ctcggaaatc atcacgcggt taaactctcc gtaatgcgga aatatcatca tcgcgtttca
    20821 ttagtgaatt c
//
LOCUS       DAZGBC010000084        20755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGBC010000084 DAZGBC010000000
VERSION     DAZGBC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.084"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..1034
                     /locus_tag="ACHK1J_002949"
     CDS             3..1034
                     /locus_tag="ACHK1J_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO7912192.1"
                     /translation="MDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQGKHGATLAD
                     ITEHSHINEYAASVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRVNMDFTQDVC
                     YQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHYYSDGAFNAA
                     LPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKENIANAGFSD
                     RIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKPGAKVCIMEL
                     FWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVAERHDNLGVG
                     HTLLICQKK"
     gene            1075..1797
                     /locus_tag="ACHK1J_002950"
     CDS             1075..1797
                     /locus_tag="ACHK1J_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO7912193.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDTFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            1794..2615
                     /locus_tag="ACHK1J_002951"
     CDS             1794..2615
                     /locus_tag="ACHK1J_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO7912194.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            2590..2847
                     /locus_tag="ACHK1J_002952"
     CDS             2590..2847
                     /locus_tag="ACHK1J_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO7912195.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            2859..3110
                     /locus_tag="ACHK1J_002953"
     CDS             2859..3110
                     /locus_tag="ACHK1J_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7912196.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPKDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            3115..3696
                     /locus_tag="ACHK1J_002954"
     CDS             3115..3696
                     /locus_tag="ACHK1J_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912197.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQPRRQAGPLQAVTQIVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLVRRQVMKRDRA"
     gene            3693..5054
                     /locus_tag="ACHK1J_002955"
     CDS             3693..5054
                     /locus_tag="ACHK1J_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFO7912198.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGLLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQCIGVLLVLDDE
                     AHLQWQNGGGHSQEQAWRRLLHPMLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            5041..5394
                     /locus_tag="ACHK1J_002956"
     CDS             5041..5394
                     /locus_tag="ACHK1J_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO7912199.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            5385..7061
                     /locus_tag="ACHK1J_002957"
     CDS             5385..7061
                     /locus_tag="ACHK1J_002957"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7912200.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            7065..7487
                     /locus_tag="ACHK1J_002958"
     CDS             7065..7487
                     /locus_tag="ACHK1J_002958"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO7912201.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            7484..8089
                     /locus_tag="ACHK1J_002959"
     CDS             7484..8089
                     /locus_tag="ACHK1J_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO7912202.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            8058..10376
                     /locus_tag="ACHK1J_002960"
     CDS             8058..10376
                     /locus_tag="ACHK1J_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO7912203.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVVLALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKFTTSLENA
                     KKEGLISNYRTIPLNSLARQEEDLHLLTTAAPTVTKALQNAGLTTVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKNSAALQEISSYYPCGIAWVDRKNTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10373..10957
                     /locus_tag="ACHK1J_002961"
     CDS             10373..10957
                     /locus_tag="ACHK1J_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO7912204.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELLNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            10959..12128
                     /locus_tag="ACHK1J_002962"
     CDS             10959..12128
                     /locus_tag="ACHK1J_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO7912205.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPVIPEKFAAHRSRNNQILLAALAQIQPQIDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSPTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQLIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            12125..12589
                     /locus_tag="ACHK1J_002963"
     CDS             12125..12589
                     /locus_tag="ACHK1J_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO7912206.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            12589..13320
                     /locus_tag="ACHK1J_002964"
     CDS             12589..13320
                     /locus_tag="ACHK1J_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO7912207.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13317..14546
                     /locus_tag="ACHK1J_002965"
     CDS             13317..14546
                     /locus_tag="ACHK1J_002965"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO7912208.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            14548..15135
                     /gene="acpT"
                     /locus_tag="ACHK1J_002966"
     CDS             14548..15135
                     /gene="acpT"
                     /locus_tag="ACHK1J_002966"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO7912209.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            15246..16820
                     /gene="nikA"
                     /locus_tag="ACHK1J_002967"
     CDS             15246..16820
                     /gene="nikA"
                     /locus_tag="ACHK1J_002967"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7912210.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            16820..17764
                     /gene="nikB"
                     /locus_tag="ACHK1J_002968"
     CDS             16820..17764
                     /gene="nikB"
                     /locus_tag="ACHK1J_002968"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO7912211.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            17761..18594
                     /gene="nikC"
                     /locus_tag="ACHK1J_002969"
     CDS             17761..18594
                     /gene="nikC"
                     /locus_tag="ACHK1J_002969"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO7912212.1"
                     /translation="MNFFISSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPNAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            18594..19358
                     /gene="nikD"
                     /locus_tag="ACHK1J_002970"
     CDS             18594..19358
                     /gene="nikD"
                     /locus_tag="ACHK1J_002970"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO7912213.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAAALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            19355..20161
                     /gene="nikE"
                     /locus_tag="ACHK1J_002971"
     CDS             19355..20161
                     /gene="nikE"
                     /locus_tag="ACHK1J_002971"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO7912214.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLERASEMLKAVDLDDSVLNKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            20167..20568
                     /gene="nikR"
                     /locus_tag="ACHK1J_002972"
     CDS             20167..20568
                     /gene="nikR"
                     /locus_tag="ACHK1J_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO7912215.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
BASE COUNT         4464 a         5843 c         5761 g         4687 t
ORIGIN      
        1 cactggatgc gattaccgaa gctcaacgca ttgcctttgc ccctatgctt tttcaaacag
       61 cactctgcct acgtaatgcg ggtgtacttt catatctgga tcggcaaggc aaacatggcg
      121 caacgctggc tgatatcacc gaacatagcc atatcaacga atatgctgcc agcgttctgt
      181 tagatatggg cttaagcggg cgaattatta cctgtaaaga gggaatttat tacctggcga
      241 aaatcggtca ttacctcctg catgacacta tgactcgcgt gaatatggat ttcacccagg
      301 atgtctgtta ccaggggctc ttcttcctcg ccgattcact caatgaagga aaaccttccg
      361 ggttaaaagt atttggcgac tggtcgacga tatatccggc actttcgcaa ttaccagacg
      421 ccgctcgtga aagctggttt gcgtttgatc attactattc agatggcgca tttaacgccg
      481 cactgcctta tgtattcgcc aacaacccca caacattgta cgatgtaggc ggcaatacgg
      541 gtaagtgggc tttgcgttgc tgcaaataca atgaaaacat cgccgtgacg ttattagatc
      601 tgccacaaca aatcgtacta gcgaaagaaa atatcgcgaa tgcaggtttt tctgatcgca
      661 tagatttcca tgcggtggat atgctaagcg atgccccatt accgggcgag gctgatatct
      721 ggtggatgag ccaattttta gactgttttt cacccgaaca aataattagc atactcagca
      781 aaattgccag cgtaatgaag cctggcgcaa aagtttgcat catggagttg ttctgggatg
      841 cgcagcggtt tgaagcagca tcatttagcc tgaacgcgtc ctcactttat tttacctgca
      901 tggcaaacgg taacagccgt ttctatagtg cagagaaatt ttacgactat ctgaataaag
      961 caggattcca ggtagcagag cgccacgata atttaggcgt tgggcataca cttttgatat
     1021 gccaaaagaa ataatcgtca cttaataatt ttggtttacc gcgggcgcgc gttgatgaaa
     1081 tttacattaa atattaccga ctggcaggca ctggcgcctg gacttagcga agcgcagcaa
     1141 tggcaagcat ggtcgcgcca gccgtgggcg attgaccctg ccgcgccgct ggcaaaatta
     1201 agtgaactgc caatgatgac cgcccgccgc ctcagttcag gcagcaaact ggctgttgaa
     1261 tgcggtctgg caatgctacg ccgccatcag ccagacgccg ttttgtatac cagtcgtcat
     1321 ggcgaactgg agcgcaatta ccgcattgtt catgcactgg caaccgaaca ggcgctttct
     1381 ccgaccgact ttgcactttc agtacacaac tcttccgtgg gtaacctgac cattgcggcc
     1441 aaacagccga tcgtttcgtc atcgctttct gctggtcgcg acactttcca gcaaggtttg
     1501 tgtgaagtgc ttagcctgtt acaagcggga taccagcgcg tgctgatggt cgattttgac
     1561 ggtttcctgc ccgagtttta tcacccacaa ctgcctgcag aaatgccgac ctggccgtac
     1621 gccgtcgcgc tggtgattga agctggcgat gactggcaat gtgaaacgca gtcagccatt
     1681 gcgggcaatg aaacaacact gccgcaaagc atgttgtttt tacagcacta tttacaaaat
     1741 gtagacacat tttcactgcc tggcgagcgc gtgcaatggc gctggagcca cagatgagta
     1801 agctgatgtc acgtctggag tggacctggc gactggtcat gaccgggtta tgttttgccc
     1861 tgtttggtct cggcggcctg ctgctttctg tcgtgtggtt taacatattg ttagttttgg
     1921 tgtgggatac ttcccgccgc cgtcgtttgg cgcgtcgcag cattgccgcc agttttcgcc
     1981 tgtttttaac tgtcgcgaaa gggcttggcg tgctggatta tcgtattgat ggcgctgaaa
     2041 ttttacgtca ggaacgcggt tgcctcgtcg tagccaatca tcccacgctg atcgattacg
     2101 tgctgctggc gtcagtcatg ccagaaactg actgcctggt gaaaagcgca ctgttaaaaa
     2161 atccttttct tggcggcgtc gtgcgggcgg cagattactt aatcaacagc gaagctgaaa
     2221 ccctgttacc ccgctgccaa cagcgactgg cacagggtga cactatcctg attttccccg
     2281 aaggcacacg aacgaagccc ggtgaaaaaa tgacgctgca gcgcggggcg gcgaatattg
     2341 ccgtgcgctg cgccagcgat ttacgcatcg tgacaatcgg ctgtagcgag catctgctgg
     2401 ataaacaaag taaatggtat gacgttccac ctgaccggcc gttttttacg gttgcagtcc
     2461 atggtcgggt tgaaatcaat aaattttacg atgcaacttc acaagaaccg gcgctggcag
     2521 caaggcagtt aaaccggcat cttttgcttc aattacaaca aggttgttta cctctctcag
     2581 gaataaataa tgcaagcgct ttatctggaa attaaaaacc tcatcatcag caccctcaat
     2641 ctggacgagt tgacgcctga tgatattgat accgacgcgc cgctgtttgg cgacggttta
     2701 gggctggact ctatcgatgc cctggaactg ggactggcag taaaaaatga gtacggcatc
     2761 gtgctctctg cggaaagcga agagatgcgt cagcactttt tctccgtcgc cactctggcc
     2821 tctttcattg ctgcgcaacg tgcctgagga aagccatcat gacagaacaa caaaccgtct
     2881 atcaggaagt ctcagccctg ctggttaagc tgtttgaaat cgatccgaag gacatcaaac
     2941 ctgaagcgcg cctgtacgaa gatctggaac tggacagcat cgacgccgtt gacatgattg
     3001 tgcacctgca aaagaaaacc ggtaagaaaa tcaagccgga agagtttaaa gcagtgcgta
     3061 cagtccagga tgtcgtcgag gctgtagaac gcctgctaca agaagcctga tctgatgtcg
     3121 ggcattcgct cgctaccgat gattaagctg ctgactggat tattgctgct cgcctggccg
     3181 tttgtgattt ggtttggctt agcgcataac ggcctgcact ggctgttacc gctaatggcg
     3241 ttgctgcttt tgttgcgtct gcgccagccc cgtcgccagg cggggccttt acaagccgtc
     3301 acacaaatcg tcgctgtcgt gggcatcgca ttatgtgtcg ccagctttat gttgaaaacg
     3361 caccagctac tgctgtttta cccggtagtg gttaacgcgg tcatgctggc ggtttttggc
     3421 ggttcgctgt ggtcggcaat gccgatagtt gagcggctgg cgcggttgca ggagccagac
     3481 ctcccggaaa agggcgtgcg ctatacccgc cacgtcacgc aaatctggtg cggctttttc
     3541 atcattaacg gtggaattgc cctctttacc gcgctttatg ccgatatgtc gttatggacc
     3601 gcctggaacg gcatgattgc ttatttgttg atggggacgt taatggctgg tgaatggctg
     3661 gtgcgccgcc aggttatgaa aagagatcgc gcatgaagca gaccttaccg cttgcacgct
     3721 ggctgactgc gccacgccct gatgacacac ccattgcgtg gctgaacgaa agtacctgga
     3781 cgctgggcga tttgcgtcac gatgtcgcgc aacttatctg ccgcttgcag caacagccgg
     3841 gtgaacgctg ggcgctgtgc tttgagaaca gctatctgtt tatcgttgcc ctgctcgcca
     3901 cgctacacgc cggaaaaaca ccggtgcttc cggggcataa ccgtgtcatc cagcttaacg
     3961 agcaacgtga actgttcgat ggtgtgctta gcgacagcga acttaactgg caaggttcat
     4021 tgctgttggt agcaagttct ccacaaatcg cgacacaatc cttcaccttt gccgctatag
     4081 cgccagaggc ttttatcgag ctgtttacct ccggttccac gggacagccg aaacgggtga
     4141 ttaaacctgt tcatttgctg gatcgtgaag cagaactgct ggctgaacga ttaggcgcac
     4201 gtctggcggg ttgtcgtgtc gtcggttccg tattgccgca gcatttgtac ggcctgactt
     4261 ttcgcgtttt cctgcccatg gcgttgggat taccgctgca cgccgccatg ctctggtatg
     4321 tcgaacagtt tgccgcgttg agtcatcagc atcgctatat cttcatcagc agcccggcat
     4381 ttttgaagcg cctggatacc cagctttctc cgccacccgt tcaggtgctt ctctctgctg
     4441 gcggtgagct gccgtggcag gatgtacaac acaccgcaag ctggctgcgc gtctggcctg
     4501 atgaaatcta cggcagcacg gaaaccggcg tcatcgcctg gcgctaccgc gaacaagagc
     4561 aacgccgctg gctacccctc ccaggtatgc agttccaggc cgaaggcgat gcctttcgcc
     4621 tcttttcacc gctgatagcg gaagataacg gcctgttact cgacgatatc ttacagttta
     4681 gcgaagatgg tcagttccat ctgatggggc gtcgtgggcg catcgttaag attgaagaaa
     4741 aacgtatttc tttgcaggaa gtggaacaac gtctgctggc actggacggc attcgcgagg
     4801 cggcggcagt tcctgttaca cgcggcgggc gtcagtgcat tggcgttttg ctggtgctgg
     4861 atgatgaagc tcatctgcaa tggcagaacg gcggcgggca tagccaggag caggcctggc
     4921 ggcgattact gcacccgatg ctggagccgg ttgctattcc gcgttactgg cgcgttattg
     4981 atgaaatgcc agtaaacagt atgaacaagc gtgtctatgc gcaattacag gagttatttc
     5041 atgaagcccc atgaaatcga gcgccatcag gcacaaacga atcaccttga aattgttctg
     5101 catctcaggg cggatctgtt ctggtttcgc ggtcattttg ccgtgcaacc attactccca
     5161 ggagttgcgc aaatcgactg ggcgatgagc tacgcgctca atctgctcgc gcctggctgg
     5221 cgttttcact ccattcagaa cataaaattc cagtctccgt tgctgccgga aaaccgcgtc
     5281 acgctcgcgc ttaactggca ggaagaacgc cagattctga gctttagcta tcagcgtcac
     5341 gacggtgacg cccgccacac cgccagtagc gggaagatcc gcctatgtcg gtaaactttt
     5401 ctccctgcgt gttgataccc tgctacaacc acggcgcgat gatgccgggc gtgctggcgc
     5461 gtcttaagcc atttaatctg ccctgtattg tagtggatga cggcagcgat gccgccacgc
     5521 aacagcaact ggacagtctg cttgccgaac agcctggcgt gaccttaatt cgcttagcag
     5581 aaaacgcagg taaaggcgcg gcagtgatgc gtggcttaca ggcagcggca gacgcagggt
     5641 tcagccatgc ggtgcaggtg gatgctgacg gtcaacatgc gattgaagat atccccaaac
     5701 tgctggcact cgctgaacaa caccctgcgg cgctgatctc cggccagcca atttacgatg
     5761 actccatccc ccgctcacgg ctttacgggc gctgggtcac ccacgtctgg gtatggatcg
     5821 aaacgctctc cctgcaactg aaagacagca tgtgcggctt tcgcgtttat ccggttgcgc
     5881 caacgctgca actggcaaaa cacgccacca tcggcaagcg gatggatttc gacactgaag
     5941 tgatggtgcg cctctactgg cagggaaaca ccagctattt cgtgccgacc cgcgtcacct
     6001 atccactgga cgggctttcg cattttgatg ccctgaaaga taacgtccgc atctcgctca
     6061 tgcacacgcg tctgtttttc ggcatgttgc cgcgtattcc ttcactgctg atgcgccgct
     6121 cttccagcca ctgggcgcgg cagagtgaag tgaaaggatt atggggaatg cgcctgatgc
     6181 tgctggtctg gcgtctgctg ggaagaacgg cgtttagcgc gctgctttac ccggtggtgg
     6241 gcgtctactg gctcactgct tctcgtgcgc gcaaagcgtc gcaagactgg ctcgcccgtg
     6301 tacgacagca tcaaccacag gcggcaaaac tcaacagcta tcagcacttt ctacgtttcg
     6361 gtaatgccat gctcgacaaa atcgccagct ggcgcggcga gctacaactg gggcgtgatg
     6421 tgctgtttgc gccaggcgca gaagcggcgc ttgacgtccg cgatccgcgc ggcaaattgc
     6481 tgctggcctc gcatcttggc gatgtggaag tgtgccgggc gctggcaaaa attcagggct
     6541 acaaaaccat taacgcgctg gtgtttagcg aaaacgccca acgctttaaa cagataatgc
     6601 aggagatggc tcctcaggca ggcattaacc tgatgccggt aacagatatc ggcccggaaa
     6661 ccgccatcct gctgaaagag aagctggata acggcgaatg ggtggcgatt gtcggtgacc
     6721 gcatcgccgt caccccgcaa cgcggcggcg actggcgcgt ctgctggagt ccgtttatgg
     6781 gccagcctgc gcccttccca caggggccgt ttattctcgc ctctattttg cgctgcccgg
     6841 tgaatctgat tttcgcccta cgccagcatg gcaagctgca tattcactgc gaaacctttg
     6901 ccgacccgct gctgttgccg cgtggcgaac gccaacaggc gctgcaaaac gctatcgacc
     6961 actacgccgc gcgtctggaa cattacgcgc tccagtcgcc cctcgactgg tttaattttt
     7021 tcgatttctg gcaactgccg gaaattcagg acaaggagta aagggtgctt aacgatcccc
     7081 gatttaccgc tgaagtcgag ctgaccattc cctttcacga cgtcgatatg atgggcgtgg
     7141 cctggcacgg taactatttt cgctactttg aagtggcccg cgaggcgctg ctcaaccagt
     7201 tcaattacgg ctatcggcag atgaaagagt ccggttattt gtggccagtg gtcgatgcgc
     7261 gggtgaaata tcgccacgcc ctcacctttg agcaacgcat ccgcgtgcgt gcgcatatcg
     7321 aagagttcga aaaccgtctg cggattggct atcagatttt cgatgccgaa acgggcaaac
     7381 gcgccaccac cggatacacc attcaggtcg cggtcgacga gcaaagtcgc gaactgtgct
     7441 ttgtcagccc cgatattctg tttgaacgca tgggagtcaa accatgaaat ttttaccgct
     7501 gctggcgctg ctgattagcc cgtttgtgag cgccctgacc ctggacgatc ttcagcaacg
     7561 ctttaccgaa caaccggtga tccgcgccca ttttgatcaa acccggacga ttaaagatct
     7621 gccgcagccg ctgcgatctc agggtcagat gttgatcgcc cgcgaccagg ggttattgtg
     7681 ggatcaaacc tcaccgttcc ccatgcagct attgctggat gataaacgca tggtgcaggt
     7741 gatcaacggt cagccgccgc aaatcatcac ggcagaaaac aacccgcaga tgttccagtt
     7801 taaccacctg ctgcgcgcgc tgttccaggc cgatcgcaaa gtgctggaac aaaacttccg
     7861 cgtcgaattt gctgacaaag gcgaaggccg ctggacgctg cgcctgacgc cgaccaccac
     7921 gccgctggat aaaattttca acaccatcga tctcgccggg aaaacctatc tggagagcat
     7981 tcaacttaat gataaacagg gcgatcgcac cgatattgct cttacccaac atcaactgac
     8041 gccagcgcaa ctgaccgatg acgaacgcca acgttttgcc gcccagtaaa cgccccgcgc
     8101 tgttatgggg gctagtctgc ctggtcatgg cggcggcgtt gctgatcctg ctgccgcaat
     8161 cacggctgaa cagtagcgtg ctggctatgt tacccaaaca ggctatgggc gatattcccc
     8221 cagcgctgaa tgacggcttt atgcagcgtc tggaccgcca actggtgtgg ctggtcagcc
     8281 ccggtaaaga ggctaatccc agggtcgctc aggagtggct gacgctgctg caaaaatccg
     8341 ctgcgctcgg cgacgttaaa gggccaatgg atgccgccag ccagcaagcg tggggagcgt
     8401 ttttctggca gcaccgcaac ggcctgattg accccgacac ccgcgcgcgc ctgcaaaacg
     8461 gcggcgaagc gcaggcgcag tggatcctct cccagcttta ttctgcgttc tccggggtaa
     8521 gcggcaagga gctgcaaaac gatccgctga tgttaatgcg cggctcgcag ctggcgatgg
     8581 cgaaaaacgg ccagcgtttg cggctgatgg acggctggct ggtgacgcag gatccccagg
     8641 gcaactactg gtatctgctg cacggtgaac tggcgggatc gtcgtttgat atgcagcaaa
     8701 cccaccagct catcacgacc ctgaatacgc tggaaaagga tctgaaaacg cgttacccgc
     8761 aggcgcagct gctttcgcgc ggcacggtgt tttacagcga ttacgccagc caacaggcga
     8821 agcaggatat ctcaaccctg ggcgtggcaa cgctgctggg ggtgatattg ctgattgtgg
     8881 cggtgttccg ctctttgcgc ccgttgctgc tttgcgtgat ttccatcggc atcggcgcgc
     8941 tggcgggaac ggtcgccact ttattgattt tcggtgaatt acacctgatg acgctggtga
     9001 tgagcatgag cgttatcggc atttccgctg actacacgct ctattatctc accgagcgga
     9061 tggtgcacgg caacgacgtt tcaccgtggc aaagcctggc gaaagtgcgc aatgccttgc
     9121 tgctggcgct gctcaccacc gtggcggcgt atctgattat gatgctcgcc cccttccccg
     9181 gcattcgcca gatggcgatt tttgccgccg tcgggttgag cgcctcctgt ctgaccgtcc
     9241 tgttctggca tccgtggctg tgccgtggcc tgccggtgcg tccggttccg gcgatggcgc
     9301 tgatgctacg ctggctggca gcgtggcggc gcaataaaaa actgtcgcgg ggtctgcccg
     9361 tcgtgctggc gctgttttcg ctggcgggga tgtcaatgct gcgcgtcgat gacgatatct
     9421 cgcagttaca ggcgctaccg cagcatattc tggcccagga gaaagccatc accgctctga
     9481 ccgggcagag cgtcgatcaa aaatggttcg tggtttacgg cgactcccca cagcaaacat
     9541 tgcggcgact ggagaaattt accacctcgc tggagaatgc gaaaaaagag gggcttatca
     9601 gcaactaccg caccattccg ctgaactccc tggcgcggca ggaggaagat ttacatctgc
     9661 tgacaacagc ggccccgaca gtaacaaaag cactgcaaaa tgccgggctg acaacagtga
     9721 acccggatct caacgccatg ccggtgaagg ttgatgaatg gctggcaagc cccgccagtg
     9781 aaggctggcg tctgctgtgg ctgacgctgg aaaatggcga aagcggcgtg ctggtgccgg
     9841 ttgaaggggt taaaaatagc gcggcgctac aggaaatctc cagctattac ccttgtggca
     9901 ttgcctgggt tgatcgcaaa aacacctttg atgaattgtt cgcactttac cgttatgtgt
     9961 taaccggctt gttgctggtg gcgctggcgg tgattgcctg cggcgcagtg gcccgtctcg
    10021 gctggcgcaa agggcttatc agcctggtgc cttcggtgct ttcgctgggc tgcggtctgg
    10081 cggtgctggc gatgagcggg caggcggtga atctcttttc gctgctggcg ctggtgctgg
    10141 tgcttggcat cggcatcaat tacacgctgt ttttcagtaa tccgcgcggc acaccattaa
    10201 cttcgctact ggcgatcaca ctggcaatgc tcaccacctt gctgacgctg ggaatgttgg
    10261 tattcagcgc cacccaggcc atcagcagtt ttggcattgt gctggtgagc ggtattttca
    10321 ccgccttcct gctttcgccg ctggctatgc ccgataaaaa gagaacaaaa aaatgatcaa
    10381 atccacattc tggcgagcgt tcgccctgac cgctacgctt atcctcaccg gctgtagcca
    10441 ctcgcaacca gaacaggaag gccgcccgca ggcgtggctg caacctggca cgcgcatcac
    10501 gctgccggca ccagggattt cacccgcagt caattcgcag caactgttga ccggcagctt
    10561 caacggcaaa acccagtcgc tgttagtgat gcttaacgcc gacgatcaga aaatcaccct
    10621 tgccgggctg tcgtcggtcg gcattcgcct gtttctggtg acctacgatg cgcaagggct
    10681 acgcgccgag caatccatcg tcgtcccaca gttaccgccc gcaagtcagg tactggctga
    10741 cgtgatgctc agccactggc cgattagcgc ctggcaaccg caacttcccg caggatggac
    10801 acttcgcgac aacggtgaca aacgcgagct gcttaacgcc agcggcaaac tggtcacgga
    10861 aatcacctat ctgaatcgcc agggaaaacg cgtgccaatc agcattgagc agcatgtctt
    10921 taaataccac atcaccattc aatacttagg tgactgatat gatttatatt tccgccgtcg
    10981 gcatgatcaa cgccctggga aataaccttg atgaaatcgc aaccaacctg gctcgcggtg
    11041 tcgcccccgg aatgcgccca cgcgcaggct ggttgcaggg gcatccgcag gcggtcctgg
    11101 ctggcgtgga tggcgagctt ccggttatcc cggaaaaatt cgctgcgcac cgatcgcgca
    11161 acaatcagat cctgctggcg gcgctggcgc agatccagcc gcagattgat gacgccattg
    11221 cgaaatatgg ccgcgagcgc attgcgattg ttcttggtac cagcacctcc ggcctgcatg
    11281 aaggcgacac gcacgttaac ctgcgcaccc acgggcagcc gagccccaca tggcactacg
    11341 cgcaacagga actcggcgat ccgtcgcgtt ttctcagcca ctggctggcg ctcgacggcc
    11401 cggcctatac cctttcaacc gcctgctctt ccagcgccag agcgataatc agcgggcgca
    11461 ggcttatcga ggcggggctg gtcgatgccg ccattgtggg tggcgcagac accctgagcc
    11521 ggatgccgat taacggtttt cacagcctcg aatcgctgtc gccaacctta tgtcagccgt
    11581 ttggtcgtga ccgcgcaggt atcaccattg gtgaaggggc ggggctaatg ctgctgaccc
    11641 gggaaccgca gctcatcgca ttgctgggcg ttggtgaatc gagtgacgct taccatattt
    11701 cggccccgca tccgcagggc gaaggggcga tccgcgccat taatcaggca ctgaccgatg
    11761 cgcagcttac gccagatgac gtcggctata tcaacctgca cggcaccgcc acgcaactca
    11821 acgaccagat tgaatcgatg gtggttaacg ccctgtttgg cgaacgcgta ccgtgcagct
    11881 ccaccaaaca tttgaccggc cacacgctgg gcgcggcggg cattaccgaa gcagcaatca
    11941 gtatgttgat cttacagcgt gatttaccgc tgcccgccca agatttcagc ctgtcgccac
    12001 gcgatcccac gctgccaccc tgcggcatta tcgaaaaacc gcagccgctg gcacgtccgg
    12061 tgatcctgtc caattccttc gcctttggcg gcaataacgc cagcattctg ctcgggaggg
    12121 tttcgtgagc cactatttat cccccggcgc ttatctgccc cacgacgcgc ccatgttact
    12181 gctggaagac gtggtgagcg tcagcgatga cagcgcggtg tgccgcgtga cggtttcgcc
    12241 cagtggcgta ctggcaccgt ttctcgaccc ggacggcaat ctccccggct ggtttgccct
    12301 tgaactgatg gcgcaaaccg tcggcgtctg gtctggctgg catcgccacc agcagggcaa
    12361 aaacagtatt gagttaggga tgatactggg cgcgcgtgaa ttgctttgcg ccgctggcat
    12421 tctgcccgca ggccagacgc tgaccattac cgtcaaactg ctgatgcagg acgatcgctt
    12481 cggcagtttt gagtgcagca tcaacgacgg cgaggcgacg ggccgcatta acaccttcca
    12541 gccgaccgcg gaagaactta ccaccctgtt tcaacaagga gcgtcctgat gagtcgttca
    12601 gttctggtta ctggtgccag caaaggcatt ggtcgcgcca tcgcctgcca actggcggca
    12661 gacggcttta acattggcgt tcactatcac cgtgacgcca caggcgctca ggaaacgctg
    12721 aatgccattg tcgccaacgg gggtaacggg cgtttgctca gtttcgacgt cgcaaaccgc
    12781 gaacagtgtc gggaagtgct ggagcatgag atcgcgcaac acggtgcctg gtacggcgtg
    12841 gtcagtaacg ccgggatcgc ccgcgatgcc gcttttccgg ctttaagcga tgacgactgg
    12901 gatgcggtga tccacaccaa cctcgacagt ttctacaacg tcattcagcc gtgcattatg
    12961 ccgatgatcg gcgcacgcca gggtgggcgc atcatcacgc tatcctcggt ttccggcgta
    13021 atgggcaatc gcggccaggt gaattacagc gctgccaaag ccggaattat cggcgcaaca
    13081 aaagcgctgg ctatcgaact cgcgaagcgc aaaatcaccg tcaactgcat cgcgccgggg
    13141 cttattgata ccgggatgat cgaaatggaa gagagcgctc tgaaagaggc aatgtcgatg
    13201 atccccatga aacgtatggg ccaggcggaa gaagttgccg ggcttgccag ttatttaatg
    13261 tctgatattg cgggttacgt cacccgccag gttatttcca tcaacggagg gatgttatga
    13321 cacgtcgcgt agtgattaca ggaatgggcg gcgtgactgc ctttggtgaa aactggcagg
    13381 acgtttctgc acgcttactg gcgtatgaaa atgcggtgcg caaaatgccg gagtggcagg
    13441 tctacgatgg cctgcacacc ctgcttggcg cgcctgttga tgatttcacg ctaccggagc
    13501 actacacccg caagcgtatc cgtgcgatgg gccgcgtgtc gcaaatgtcg acccgtgcca
    13561 gcgagctggc gttagagcag gcagggttga ttggcgatcc gatattaacc agcggcgaaa
    13621 cgggcattgc gtacggttca tcgaccggca gtaccggccc ggtaagcgag ttcgccacca
    13681 tgctgacgga aaagcacacc aataacatca ctggcaccac ctatgtgcag atgatgccgc
    13741 acaccaccgc cgttaatact ggcctctttt tcggcctgcg cgggcgggtg atcccaacct
    13801 ccagcgcctg tacttccggc agccaggcaa tcggttatgc gtgggaagcc attcgccacg
    13861 gttatcaaac ggtgatggtt gctggtggtg ccgaagagtt atgtccttca gaagcagcgg
    13921 tatttgatac gcttttcgcc accagccagc acaatgatgc gccgaaaacc acaccttcac
    13981 cgttcgatga aaaccgcgac gggctggtga ttggtgaagg cgcaggtacg ctcattctcg
    14041 aagaactgga gcacgccaaa gcgcgcggcg cgaccattta cggtgaaatc gtcggctttg
    14101 cgactaactg cgacgcggca catattaccc agccgcagcg cgaaaccatg cagtattgta
    14161 tggagcaatc gctgaaaatc gccgggttaa gcgcacaaga tatcggctat atttcagcgc
    14221 atggtacggc gaccgatcgc ggtgatatgg cagaaagtct ggcgacagca accatttacg
    14281 gtgataatgt gccgctctcc tcgctgaaaa gttattttgg tcatacgctt ggtgcctgtg
    14341 gcgcgctcga agcctggatg agtctgcaaa tgatgcgcga aggttggttt gcgcctacgc
    14401 taaacctgaa taaaccagac ccgaattgtg gcgcgctgga ttacatcatg catgaagccc
    14461 gcaaggttga ttgtgagttt ttgcagagta acaactttgc ctttggcggc attaatacat
    14521 ccatcatcat taaacgttgg ccctgatatg tatcggatag ttctggggaa agtttcgacc
    14581 ttaagcgcag ctccactgcc accgggttta cgcgagcaag caccgcaagg tccacgacgc
    14641 gaacgctggc tggcggggcg tgcattgctt tcgcacacgc tttccccgct accggagatc
    14701 atctatggcg aacaaggcaa acctgcattc gcgccggaaa cgccgctatg gttcaactta
    14761 agccatagcg gtgacgatat cgccctgctg ttgagtgatg aaggcgaagt cggctgcgat
    14821 atcgaagtga ttcgcccgcg cgccaactgg cgctggctgg cgaacgccgt gttcagcctc
    14881 ggggaacacg ctgaaatgga cgcagtgcat cctgatcagc aactggaaat gttctggcgc
    14941 atctggacgc gcaaagaagc catcgttaaa cagcgtggcg gcagcgcctg gcaaatcgtc
    15001 agcgtagata gcacctatca ctcctcgctg tcagtcagcc attgtcagct agaaaattta
    15061 agccttgcga tctgcacccc cacccccttt acgctcaccg ccgacagtgt gcaatggatc
    15121 gattcagtta actgatccgc ccacccgact gcccatctat tgatccagaa caggtaatca
    15181 gtatgacgaa tacttaaaat cgtcatactt atttccgcca tctattttaa tccattgggg
    15241 ttaccatgct ctccacactc cgccgcactc tatttgcgct gctggcttgt gcgtctttta
    15301 tcgtccatgc cgctgcacca gatgaaatca ccaccgcctg gccggtgaat gtcgggccac
    15361 taaacccgca cctttacacg cctaaccaga tgttcgccca gagcatggtt tatgaaccgc
    15421 tggtgaaata tcaggctgat ggttcggtga tcccgtggct ggcaaaaagc tggactcatt
    15481 cagaagatgg taaaacctgg accttcaccc tgcgtgatga cgtgaaattc tctaacggcg
    15541 aaccgtttga tgcagaggcg gcggcagaaa acttccgcgc agtgctcgat aaccgtcaac
    15601 gtcacgcctg gctggagctg gcaaaccaga ttgttgatgt caaagcactc agtaaaacag
    15661 agctgcaaat caccctgaaa agcgcctact atcctttcct gcaagaactg gccctgccgc
    15721 gccctttccg ctttatcgcc ccctcgcagt ttaaaaacca tgaaaccatg aacgggatta
    15781 aagcgccgat tggcaccggg ccgtgggttt tgcaggaatc gaaactgaat cagtacgatg
    15841 tcttcgtccg taacgaaaac tactggggcg aaaagccggc aattaagaag atcactttta
    15901 acgtcatccc ggacccgact acccgcgcgg tggcgtttga aactggcgat atcgacctgc
    15961 tgtacggaaa cgaagggtta ttaccgctcg ataccttcgc ccgctttagc cagaacccgg
    16021 cttaccatac ccaactgtca cagccgatcg aaaccgtgat gctggcgctt aataccgcca
    16081 aagcccccac caatgagctg gcagtacgtg aagctcttaa ttacgcggta aacaaaaaat
    16141 cgctgattga taacgcgttg tatggcaccc agcaggtcgc cgacaccctg tttgcccctt
    16201 ctgtgcccta cgccaatctc ggcctgaaac cgcgccagta cgatccgcag aaagcgaaag
    16261 cgttgctgga aaaagccggt tggacgctgc ctgcgggcaa agacatccgc gagaaaaatg
    16321 gtcagccgct gcgcattgaa ctgtcgttca tcggcaccga tgcgttaagc aaatcgatgg
    16381 cggaaatcat tcaggctgat atgcgccaga ttggcgcaga tgtctcgctg attggcgaag
    16441 aagagagcag tatctatgct cgtcagcgcg acggtcgttt tggcatgatt ttccaccgca
    16501 cctggggcgc gccatacgac ccacacgcct tcctgagttc aatgcgcgta ccgtcacacg
    16561 ctgacttcca ggcacagcaa ggattagccg acaaaccgct gattgataaa gagatcggcg
    16621 aagtgctagc gacccatgac gaaacgcaac gtcaggcgct gtatcgcgac attctgaccc
    16681 gtctgcatga cgaggcggtt tatctgccta tcagttacat ctcaatgatg gtggtatcaa
    16741 aaccggagct gggtaacatc ccctacgcgc cgatcgccac cgaaattccg ttcgaacaga
    16801 ttaaaccggt gaaaccttaa tgttgcgtta cgtattacgc cgctttctgc tgctgatccc
    16861 gatggtgctc gccgcctcgg tgatcatttt tctgatgctg cgccttggta ccggcgaccc
    16921 ggcgctcgat tatttgcgtc tgtctaacct gccgccgacg ccagagatgc tggcctctac
    16981 ccgcaccatg ctcggactgg atcagccgct gtacgtccag tacggtacct ggttgtggaa
    17041 ggcgctgcat cttgactttg gtatctcatt cgccagccaa cgcccagtgc tggacgatat
    17101 gctgaacttc ctgcccgcca cgctggaact tgcaggtgcc gcgctggtat taattctgct
    17161 cacttctgtg ccgctcggta tctgggcggc gcgccatcgc gatcgtctgc cggatttcgc
    17221 cgtacgtttc atcgcgtttc tcggcgtgtc gatgcctaac ttctggctgg cgtttttact
    17281 ggtgatggcg ttttcggtgt atctgcaatg gctacccgcg atgggttacg gcggctggca
    17341 gcacatcatt ttgcctgcgg tttccattgc ctttatgtcg ctggcgatta acgcacggct
    17401 gctgcgcgcc agtatgctgg acgtcgccgg tcagcgtcac gtcacctggg cgcgtctgcg
    17461 cggcctgaac gacaaacaga ccgaacgtcg ccacatcctg cgcaatgcct cgctgccgat
    17521 gatcaccgcc gtggggatgc acattggcga actgattggc gggacgatga ttatcgaaaa
    17581 catctttgcc tggcctggcg tcgggcgcta tgcggtgtcg gcgattttta accgtgacta
    17641 tccggtgatc cagtgcttta cgctgatgat ggtggtggtt tttgtggtct gtaatttgat
    17701 tgtcgatttg ctcaacgccg cgctggaccc gcgcattcgt cgtcatgaag gagcgcacgc
    17761 gtgaactttt tcatctcttc ccgctggtcg gtacgcctgg cgctgatcat tatcgccctg
    17821 ctggcgctga ttgcgctcac cagccagtgg tggctgccgt atgaccccca ggcgattgat
    17881 ttgccgtcgc gcctgctttc gccgaatgcg cagcactggc tgggcaccga tcacttaggt
    17941 cgcgatattt tctcgcggct gatggcagcg acccgcgtgt cgctcggttc ggtaatggcc
    18001 tgcctgctgc tggtgctgac attagggctg gttattggcg gcagcgccgg gctgattggc
    18061 gggcgcgttg atcaggctac catgcgcgtc gccgatatgt ttatgacctt cccgacctcg
    18121 attctgtcgt tctttatggt tggcgtgctc ggcactgggc tgaccaacgt aattatcgcc
    18181 atcgccctgt cgcactgggc gtggtatgca cgcatggtgc gcagcctggt gatttcactg
    18241 cgccagcgcg agtttgtgct ggcatcacgg ctttctggcg cgggccatgt gcgagtgttt
    18301 gttgatcatc tggcaggtgc ggtgatccct tcgctgctgg tgctggcaac gctggatatc
    18361 ggccatatga tgctgcacgt cgcggggatg tccttcctcg gcctcggcgt gaccgcgccg
    18421 accgccgaat ggggcgtgat gattaacgac gcgcgccagt atatctggac ccagccgctg
    18481 caaatgttct ggccggggct ggcgctgttt atcagcgtga tggcctttaa cctggtgggt
    18541 gacgcactgc gcgatcatct ggaccctcat ctggtgacgg agcacgcaca ctaatgccgc
    18601 aacagattga actacgtaat atcgcgctac aggccgcgca gccgctggtg cacggcgtat
    18661 cgttaaccct gaaacgcggg cgtgtgctgg cgttagtcgg cggtagcggc agcgggaaat
    18721 cattaacctg cgccgcggcg ctgggaattc tgcccgcagg cgttcgccag acggcggggg
    18781 aaattttagc cgatggcaaa ccggtttctc cctgcgccct gcgcggcatc aaaattgcca
    18841 ccatcatgca gaacccgcgc agcgccttta atccgctacg caccatgcac acccacgcgc
    18901 gtgaaacctg cctggcgctg gggaaacccg ccgatgacgc tacgcttacc gctgccatag
    18961 aagcggtggg gctggaaaac gccgcacgcg tgctgaagct gtacccgttc gagatgagcg
    19021 gcggcatgtt gcagcgcatg atgattgcga tggcggtgct ttgcgaatca ccgtttatca
    19081 tcgccgatga accaaccacc gacctcgacg tggtagcaca ggcgcgcatc ctcgatctgc
    19141 tggaaagcat tatgcaaaaa caggcaccgg ggatgctgct ggtcacccat gatatgggcg
    19201 tagtggcgcg tctggcggat gacgtggcgg tgatgtcgca cggtaagatt gtcgaacagg
    19261 gcgatgtcga aacgctgttt aacgccccca aacatacggt gacgcgcagc ctggtttccg
    19321 ctcatctcgc cctctacggt atggagctgg catcatgact ttacttaacg tttccggcct
    19381 ttcccatcac tatgcgcacg gtggatttag cggaaaacat cagcaccagg cggtgctgaa
    19441 taacgtttcc ctgaccctga aaagcggcga aaccgtcgcc ctgctggggc gcagcggctg
    19501 cgggaaaagc accctcgcgc ggctgctggt aggcttagag tctccctcac aagggaatat
    19561 tagctggcgc ggcgaatcgt tggcaaagct caatcgcgcc cagcgtaaag cgttccgccg
    19621 cgatattcag atggtatttc aggactccat cagcgccgtg aatccgcgca aaaccgtgcg
    19681 cgagatcctg cgagaaccga tgcgtcacct gctatcactg aaaaaatccg aacaactgga
    19741 gcgcgccagc gaaatgctga aggcggtcga tctcgacgac agcgtgctca acaagcgccc
    19801 accgcagtta agcggcggcc agctccagcg cgtctgcctg gctcgcgcgc tggcggtcga
    19861 accgaaactg ctgattctgg atgaagcagt ttctaacctt gatctcgtgt tacaggcggg
    19921 cgtcattcgc ctgctgaaaa agctacaaca acagtttggc accgcctgcc tgttcatcac
    19981 ccacgactta cgcctggtgg aacgcttttg ccagcgagtg atggtgatgg acaacggaca
    20041 aatcgtcgaa acccaggcgg tgggagataa attaaccttt tcctctgacg ccggacgtgt
    20101 gctacaaaac gcggtattac ccgcattccc cgtgcgccgt cgcacctcag aaaaggttta
    20161 acgcaaatgc aacgagtcac catcacgctt gatgacgatt tactggagac gctggacagc
    20221 ctgagccagc gtcgtggtta taacaaccgt tccgaagcta tccgcgatat tctgcgtagc
    20281 gccctggcgc aggaagctac ccagcagcac ggcacacaag gtttcgcggt gctgtcgtat
    20341 gtgtatgaac acgaaaaacg cgacttagcc agccgtattg tttccaccca gcatcatcac
    20401 cacgacctct ccgtcgccac gctacatgtg cacatcaacc acgacgactg tctggaaatc
    20461 gccgtgttga aaggtgacat gggtgacgtg cagcattttg ccgatgacgt tatcgcccag
    20521 cgcggcgtgc ggcacgggca tttgcagtgc ttgccgaagg aagattgagt ctattattgg
    20581 gtcctttggt gaacgatcaa caaagggcca ctagcacacc tgattcctct ctaaataccc
    20641 tatccgaacc ttctcttttg taacgttcta aatatattcc taaaaatctt caattcattg
    20701 tgaccacaag tttttcttcg ctttttcgta tgaagatact gtcattaaaa taata
//
LOCUS       DAZGBC010000085        20411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGBC010000085 DAZGBC010000000
VERSION     DAZGBC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.085"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..979
                     /gene="mdtN"
                     /locus_tag="ACHK1J_002973"
     CDS             <1..979
                     /gene="mdtN"
                     /locus_tag="ACHK1J_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO7912216.1"
                     /translation="VALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIVEL
                     AVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVN
                     ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSA
                     ASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPI
                     FTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGG
                     LVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            979..3030
                     /gene="mdtO"
                     /locus_tag="ACHK1J_002974"
     CDS             979..3030
                     /gene="mdtO"
                     /locus_tag="ACHK1J_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO7912217.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGVQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQVLNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICHTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATGSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLTVALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            3027..4493
                     /gene="mdtP"
                     /locus_tag="ACHK1J_002975"
     CDS             3027..4493
                     /gene="mdtP"
                     /locus_tag="ACHK1J_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO7912218.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKNSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVLQSIQLMKSLGGGYQAGPVVEKK"
     gene            4691..6838
                     /gene="fdhF"
                     /locus_tag="ACHK1J_002976"
     CDS             4691..6838
                     /gene="fdhF"
                     /locus_tag="ACHK1J_002976"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5108..5110,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO7912219.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            6932..7621
                     /gene="yjcO"
                     /locus_tag="ACHK1J_002977"
     CDS             6932..7621
                     /gene="yjcO"
                     /locus_tag="ACHK1J_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO7912220.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7926..9239)
                     /gene="gltP"
                     /locus_tag="ACHK1J_002978"
     CDS             complement(7926..9239)
                     /gene="gltP"
                     /locus_tag="ACHK1J_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO7912221.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9581..10177)
                     /gene="nrfG"
                     /locus_tag="ACHK1J_002979"
     CDS             complement(9581..10177)
                     /gene="nrfG"
                     /locus_tag="ACHK1J_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO7912222.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10174..10557)
                     /gene="nrfF"
                     /locus_tag="ACHK1J_002980"
     CDS             complement(10174..10557)
                     /gene="nrfF"
                     /locus_tag="ACHK1J_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO7912223.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10550..12268)
                     /gene="nrfE"
                     /locus_tag="ACHK1J_002981"
     CDS             complement(10550..12268)
                     /gene="nrfE"
                     /locus_tag="ACHK1J_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO7912224.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(12288..13244)
                     /gene="nrfD"
                     /locus_tag="ACHK1J_002982"
     CDS             complement(12288..13244)
                     /gene="nrfD"
                     /locus_tag="ACHK1J_002982"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO7912225.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13241..13912)
                     /gene="nrfC"
                     /locus_tag="ACHK1J_002983"
     CDS             complement(13241..13912)
                     /gene="nrfC"
                     /locus_tag="ACHK1J_002983"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO7912226.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13909..14475)
                     /gene="nrfB"
                     /locus_tag="ACHK1J_002984"
     CDS             complement(13909..14475)
                     /gene="nrfB"
                     /locus_tag="ACHK1J_002984"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO7912227.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14520..15956)
                     /gene="nrfA"
                     /locus_tag="ACHK1J_002985"
     CDS             complement(14520..15956)
                     /gene="nrfA"
                     /locus_tag="ACHK1J_002985"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO7912228.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16349..18307
                     /gene="acs"
                     /locus_tag="ACHK1J_002986"
     CDS             16349..18307
                     /gene="acs"
                     /locus_tag="ACHK1J_002986"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO7912229.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18440..18754
                     /gene="yjcH"
                     /locus_tag="ACHK1J_002987"
     CDS             18440..18754
                     /gene="yjcH"
                     /locus_tag="ACHK1J_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO7912230.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18751..20400
                     /gene="actP"
                     /locus_tag="ACHK1J_002988"
     CDS             18751..20400
                     /gene="actP"
                     /locus_tag="ACHK1J_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO7912231.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGCWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         4820 a         5593 c         5450 g         4548 t
ORIGIN      
        1 ggttgcgttg gcgctggttg cccttgtttt cgttatctgg cgcgtagaca gtgcgccatc
       61 aactaatgac gcttacgcgt cagcagatac cattgatgtg gtgccggaag tcagcggccg
      121 cattgtagaa ctggcggtca ccgacaacca ggcagtcaaa cagggcgatt tgctgttccg
      181 cattgacccg cgcccgtacg aagccaatct ggcgaaagct gaagcctccc tcgcggcact
      241 ggataagcaa attatgctca cccagcgtag cgttgacgcg caacagtttg gtgccgactc
      301 ggttaacgcc acggtagaaa aagcccgtgc cgccgctaaa caggccacag atacattacg
      361 ccgcaccgag ccattactga aagaaggttt tgtctcagcg gaagacgttg accgtgcgag
      421 gaccgcgcag cgcgctgcag aagcggatct taatgccgta ttgttacagg cgcagtcagc
      481 cgccagcgcc gtcagcggcg tcgatgcgct ggtggcgcaa cgtgcggcgg tcgaagcgga
      541 tattgccctg accaaactgc atctggaaat ggcgaccgtt cgcgcaccgt ttgatggccg
      601 ggtcatttcc ctcaaaacct ccgtcgggca atttgcttct gccatgcgcc ctatttttac
      661 cctaatcgac actcgtcact ggtacgtgat cgccaacttc cgcgaaaccg atctgaaaaa
      721 tattcgctca ggtacacccg caacgattcg cctgatgagt gacagcggca aaactttcga
      781 gggtaaagtg gattcgattg gctacggcgt gctaccggat gacggcggcc tggtgctggg
      841 cggcctgcca aaagtttctc gctctattaa ctgggtccgc gttgcccagc gttttccggt
      901 caaaatcatg gtcgataaac ctgacccgga aatgttccgc atcggcgctt cggcagtcgc
      961 taatcttgag ccgcaataat gagcgcgctc aactccctgc cattaccggt ggtcaggctg
     1021 ctggcattct ttcatgaaga gttaagcgag cggcgaccag gtcgcgtgcc gcagaccgtg
     1081 caactctggg taggctgcct gctggtgatt ctgatctcga tgacttttga gatccctttt
     1141 gtggcgttat cgctggcagt gctgttttac ggtgttcagt cgaacgcgtt ttacaccaaa
     1201 tttgtcgcga tcttgtttgt ggttgccacg gtgctggaga tcggcagcct gtttttgatc
     1261 tacaaatggt catacggcga accgttgatc cgcttgatca tcgccgggcc gatcctgatg
     1321 ggctgtatgt ttttgatgcg cacccatcgg ttggggctgg tctttttcgc cgtcgccatt
     1381 gtcgctattt acgggcaaac cttccccgcc atgctcgact atccagaagt ggtcgtgcgc
     1441 ttaacgctgt ggtgtatcgt tgttggcctc tatccaacct tgctgatgac gttaatcggc
     1501 gtactgtggt ttcccagtcg tgccatttcg caaatgcatc aggtgcttaa tgatcggctt
     1561 gatgatgcca ttagccacct gacggacagc ctcgcaccgc tacccgaaac gcggattgaa
     1621 agagaggcgc tggcgctgca aaaactcaat gtcttttgcc tcgcggacga tgccaactgg
     1681 cgaacccaga gtgcatggtg gcaaagctgc gtggcaacgg taacctacat ttactcgacg
     1741 ctgaatcgct acgatcccac ctcttttgct gattctcagg caattattga attccgacaa
     1801 aaattagctt cagaaatcaa caagctgcag catgccatta ccgaaggtca gtgctggcaa
     1861 agcgactggc ggatcagtga aagtgaagcg atggcggcac gggaatgtaa cctggagaat
     1921 atctgccaga cgctgttaca gcttggtcag atggacccga atacgccgcc aacgcccgca
     1981 gccaaaccgc catcaatggc cgccgatgct tttaccaatc cagactatat gcgctacgcg
     2041 gtaaaaacgc tgctcgcctg tttgatctgt cacaccttct acagcggcgt ggactgggaa
     2101 ggcattcaca cctgtatgct gacctgcgtg atcgtcgcta atccgaatgt cggttcatcg
     2161 taccagaaga tggtgctgcg ttttggcggg gccttttgcg gcgcgattct ggcgctgtta
     2221 ttcacgctac tggtcatgcc ctggctggac aatattgtcg aattgctgtt tgtgctggca
     2281 ccgattttcc tgttgggcgc atggattgcc accggctctg aacgctcttc ttatatcggc
     2341 acacagatgg tggtcacctt cgcgctcgcc acgctcgaaa acgtttttgg cccggtgtac
     2401 gacctggtgg aaattcgcga tcgcgccctg ggtatcatca ttggtaccgt ggtgtccgcg
     2461 gtgatttaca cctttgtctg gcctgaaagt gaagcgcgca cgctgccgca aaaactgact
     2521 gtcgcgctgg gtatgttaag taaagtaatg cggatcccac gccagcagga agtcacggct
     2581 ctgcgcactt atctgcaaat tcgtataggt ctgcatgcgg cgtttaatgc ctgtgaagag
     2641 atgtgccaac gcgtggcgct ggagcgtcaa ctggacagcg aagaacgcgc cttactgatt
     2701 gaacgttcgc aaacggttat tcgtcagggc cgcgatcttc ttcacgcctg ggatgccacc
     2761 tggaactcgg cgcaggcgct ggataacgca ctacagccgg acagagcagg tcagtttgcc
     2821 gacgccctgg aaaaatacgc tgccggtctg gcaaccgcac tcagccgttc tcctcaaata
     2881 acgcttgaag agacacccgc ctctcaggcc atcctgccca ccttattaaa acaggagcaa
     2941 cacgtctgcc agcttttcgc ccgcttgcca gactggacag ccccggcatt aacacccgcc
     3001 acggaacagg tacaaggagc cacgcaatga tcaatcgtca actttcacgt ctgctgttgt
     3061 gcagcattct cggcagcacg acgctgattt ccggctgtgc cctggtacgt aaggattctg
     3121 cgcctcatca acagctcaaa ccggaacaaa tcaaactggc cgatgatatt catcttgcca
     3181 gctccggctg gccgcaggcg cagtggtgga aacaactcaa tgacccgcag ttggatgccc
     3241 tgatccagcg gacgctaagc ggttcacaca ccctcgcaga agcgaaactg cgggaagaaa
     3301 aagcgcagtc ccaggccgat ttgttagatg ccggttcaca gttgcaggtg gcagcgttag
     3361 ggatgctcaa ccgccaacgt gtctcggcga acggcttttt aagcccttat gcgatggatg
     3421 cgccagcact ggggatggac gggccgtact atacggaagc cacagtaggt ttgtttgccg
     3481 gactggatct tgatttgtgg ggtgtgcatc gctcagcggt tgccgccgcc attggcgcgc
     3541 ataatgccgc gctggcagaa accgcagcag tagagctatc gctgaccacg ggcgtagcgc
     3601 agctttatta cagtatgcag gccagctatc agatgctcga tctgttagaa caaactcgcg
     3661 atgtgattga ttacgcggtg aaagcgcacc aaagtaaagt ggcacacggt ctggaagcgc
     3721 aagtgccttt ccacggcgcg cgggcacaga ttctggcggt cgataaacaa attgctgccg
     3781 ttaaagggca aatcaccgaa acgcgagaat ctctgcgcgc attgattggc gcgggagcca
     3841 gcgatatgcc ggagatcaaa ccggtggcat taccgcaagt ccagactggc attccggcga
     3901 cgctctctta tgagttgctc gccagacgcc cggatctgca agccatgcgc tggtatgttc
     3961 aggcgtcatt agatcaggtg gattccgcgc gggcgctgtt ctacccaagc tttgatatca
     4021 aagcgttttt cggccttgat gccatccacc tggatacctt attcaaaaaa aacagtcgcc
     4081 agttcaactt catcccgggt ctgaaattgc cgctgtttga cggtggacgg ttgaatgcca
     4141 atctcgaagg cactcgcgcc gccagcaaca tgatgattga acgttacaac cagtcagtac
     4201 tgaacgcggt gcgtgacgtt gccgtcaacg gcacgcgtct gcaaacgctc aacgacgagc
     4261 gagaaatgca ggctgaacgc gtggaagcaa cgcgcttcac ccagcgcgct gccgaggccg
     4321 cctatcagcg cggcttaacc agccgcttac aggccactga agcccggttg ccagtacttg
     4381 ccgaagagat gtcattactg atgctggaca gccgccgggt gctccaaagc attcagttga
     4441 tgaaatcgct gggcggcggg tatcaggcag gtcccgtcgt cgagaaaaaa taaaatgtct
     4501 gccgcgtgat ggctgtcacg cggtatttcg tttcgtcacg tcaaaactga cgacagcctg
     4561 tttttcgtca gagttttgaa taaatagtgc ccgtaatatc agggaatgac cccaaataaa
     4621 atgtggcata aaagatgcat actgtagtcg agagcgcgta tgcgtgattt gattaactgg
     4681 agcgagaccg atgaaaaaag tcgtcacggt ttgcccctat tgcgcatcag gttgcaaaat
     4741 caacctggtc gtcgataacg gcaaaatcgt ccgggcggag gcagcgcagg ggaaaaccaa
     4801 ccagggtacc ctgtgtctga agggttatta tggctgggat ttcattaacg atacccagat
     4861 cctgaccccg cgcctgaaaa cccccatgat ccgtcgccag cgtggcggca aactcgaacc
     4921 tgtttcctgg gatgaggcac tgaattacgt tgccgagcgc ctgagcgcca tcaaagagaa
     4981 gtacggtccg gatgccatcc agacgaccgg ctcctcgcgt ggtacgggta acgaaaccaa
     5041 ctatgtaatg caaaaatttg cgcgcgccgt tattggtacc aataacgttg actgctgcgc
     5101 tcgtgtctga cacggcccat cggttgcagg tctgcaccaa tcggtcggta atggcgcaat
     5161 gagcaatgct attaacgaaa ttgataatac cgatttagtg ttcgttttcg ggtacaaccc
     5221 ggcggattcc cacccaatcg tggcgaatca cgtaattaac gctaaacgta acggggcgaa
     5281 aattatcgtc tgcgatccgc gcaaaattga aaccgcgcgc attgctgaca tgcacattgc
     5341 actgaaaaac ggctcgaaca tcgcgctgtt gaatgcgatg ggccatgtca ttattgaaga
     5401 aaatctgtac gacaaagcgt tcgtcgcttc acgtacagaa ggctttgaag agtatcgtaa
     5461 aatcgttgaa ggctacacgc cggagtcggt tgaagatatc accggcgtca gcgccagtga
     5521 gattcgtcag gcggcacgga tgtatgccca ggcgaaaagc gccgccatcc tgtggggcat
     5581 gggtgtaacc cagttctacc agggcgtgga aaccgtgcgt tctctgacca gcctcgcgat
     5641 gctgaccggt aacctcggta agccgcatgc gggtgtaaac ccggttcgtg gtcagaacaa
     5701 cgtgcagggt gcctgcgata tgggcgcgct gccggatacg tatccgggat accagtacgt
     5761 gaaagatccg gctaaccgcg agaaattcgc caaagcctgg ggtgtggaaa gcctgccagc
     5821 gcataccggc tatcgcatca gcgagctgcc gcaccgcgca gcgcatggcg aagtgcgtgc
     5881 cgcgtacatt atgggcgaag atccgctgca aactgacgcg gagctgtcgg cagtacgtaa
     5941 agcctttgaa gatctggaac tggttatcgt tcaggacatc tttatgacca aaaccgcgtc
     6001 ggcggcggat gttattttac cgtcaacgtc gtggggcgag catgaaggcg tgtttactgc
     6061 ggctgaccgt ggcttccagc gtttcttcaa ggcggttgaa ccgaaatggg atctgaaaac
     6121 ggactggcaa atcatcagtg aaatcgccac ccgtatgggt tatccgatgc actacaacaa
     6181 cacccaggag atctgggatg agttgcgtca tctgtgcccg gatttctacg gtgcgactta
     6241 cgagaaaatg ggcgaactgg gcttcattca gtggccttgc cgcgatactt cagatgccga
     6301 tcaggggact tcttatctgt ttaaagagaa gtttgatacc ccgaacggtc tggcgcagtt
     6361 cttcacctgc gactgggtag cgccaatcga caaactcacc gacgagtacc cgatggtact
     6421 gtcaacggtg cgtgaagttg gtcactactc ttgccgttcg atgaccggta actgtgcggc
     6481 actggcggcg ctggctgatg aacctggcta cgcacaaatc aataccgaag acgccaaacg
     6541 tctgggtatt gaagatgagg cattggtttg ggtgcactcg cgtaaaggca aaattatcac
     6601 ccgtgcgcag gtcagcgatc gtccgaacaa aggggcgatt tacatgacct accagtggtg
     6661 gattggtgcc tgtaacgagc tggttaccga aaacttaagc ccgattacga aaacgccgga
     6721 atacaaatac tgcgccgtgc gcgtcgagcc gatcgccgat cagcgcgccg ccgagcagta
     6781 cgtgattgac gagtacaaca agttgaaaac tcgcctgcgc gaagcggcac tggcgtaata
     6841 cagtcctttc tacagcctcc tttcggaggc tgttttttta tccattcgaa ctctttatac
     6901 tggttactcc ctacccaatc gtattatcaa aatgaaaaaa attatcgcat tgatgttgtt
     6961 tttgacattc tttgcccacg cgaacgactc cgagcctggc agccagtatt taaaggcagc
     7021 agaggccggg gaccgacgcg cacaatattt tcttgccgac agctggttta gctccggcga
     7081 tttgagcaaa gccgaatatt gggcacagaa agccgccgac agcggtgatg ctgatgcctg
     7141 cgcgctgctg gcgcagatca aaatcaccaa tccggtcagt ctggactatc cacaagcaaa
     7201 agttcttgca gagaaagcgg cgcaagcggg cagtaaagaa ggtgaagtaa cgctggcgca
     7261 tattctggta aatactcaag cgggcaaacc ggattatcca aaggcgattt cgctgttaga
     7321 aaacgcctcg gaagatctgg agaacgactc tgccgtcgat gcccaaatgc tgcttggttt
     7381 gatttacgcc aacggcgtgg gcattaaggc cgacgatgac aaggcaacct ggtatttcaa
     7441 acgcagctct gcaatttccc gaaccggtta ttccgagtac tgggcgggaa tgatgttctt
     7501 aaacggtgaa gaaggtttta tcgagaagaa caagcaaaag gcgctgcact ggttgaacct
     7561 gagctgtatg gaagggtttg ataccgggtg tgaagagttt gaaaaattaa ccaacggtta
     7621 aggtaggcct gataaggcgc gttaagcgtc gcatcaggcg ttgatgtcgg atgcggcgta
     7681 aacgccttat ccgacctaca aaatcacgct aaatcaggct attgcgctgt ctggcgtagg
     7741 cctgataaga cgcggcaagc gtcgcatcag gcattgatgt cggatgcggc gtaaacgcct
     7801 tatccgacct acaaaatcac gctaaatcag gctattgcgc tgtctggcgt aggcctgata
     7861 agacgcggca agcgtcgcat caggcattca cacattgccg gggggatatc cccggcaatc
     7921 ttcaattatt gatccgcagt tttatcaaat ttgcccagca cttcacgctc ataagccagc
     7981 gcctttttgc ggtcaaattt gtgttcccac ttggcaatca ccagcaccgc cagcgcatta
     8041 cccaccacgt tcagcgcagt acgcgccatg tcgaggatac ggtcaacacc agcaataaac
     8101 gccagacctt ccagcggaat acctacgcta cccagcgttg ccagcaacac cacaaacgac
     8161 acgcccggca cgccagcaat ccctttcgag gtcaccatca gcgtcagcac cagaatgatt
     8221 tcctgccaga tggacaggtc aatgccatac agctgagcga tgaaaatagc ggcaatactt
     8281 tgatacagcg tcgaaccatc aaggttaaaa gagtaaccgg tcggcaccac gaagctggtg
     8341 atcgacgccg gtgctccgta ggcttccatc ttctcaataa ttcgcggcag tacgctttca
     8401 gagctggcag tggagtacgc cagaatcagc tcatctttca gaatacgaat caggatccag
     8461 acgcttaacc cgcacaggcg cgccacaatt cccagcacta ccagcgcgaa gaacagaatg
     8521 gcgaaatgca ccagcagcac cagtttcgcc agtggccaca gagaagagaa accaaagtta
     8581 gccaccgtca ccgcaatcag cgcaaacaca cccaccggcg cataacgcat caccatgtga
     8641 gtcactttaa acatggtttc agagatggag cggaacacgg tcaccagcgg ttcacgatgc
     8701 gtcgcgggca gggaggaaag ccccagacca aacagcaccg agaagaagat gatcggcagc
     8761 atttcgcctt tcgccatcga cgccacaata ttcgtcggca ccagcgacaa aatcgtgccc
     8821 ataatgccgt gggaactgct ttgtaccgct tccgtagtgc tctgatattt cgagatatcg
     8881 acggtcgcca actgcgacat atccaccccg gcaccgggct ggaagacgtt cgccagagtg
     8941 atccccaaaa tgatggcgac ggtggtgatc acctcgaagt agataatggt tttcgcgcca
     9001 atacgcccga gctgtttggc atcaccaaca cccgcgatac ccaccaccag cgtggagatc
     9061 acaatcggca caacaatcat tttgatcaga tggatgaaga tatcacccgc cggagagagc
     9121 aaattgacga ccagccagtc gcggctgtcg ctatggtagt gcaggtagct tcccaggaga
     9181 atgcccagca ccatagcaaa cagaatctgc caggccaggc tgaattttat atttttcatc
     9241 agaataactt cctcaatgga gcgccgttcc ccgttcagaa acgcgagaac gtgacatact
     9301 gccagggcat gaattgtgtt gcgttggttt gtagattttt ttaacgctta tgatcagtat
     9361 catttgcgcg gtatgttgcg caacccttta acaacagagc ttttcgctgc attaatgcat
     9421 aataacgcga gtttatggca ggattaatga ataaccattt gttatggaaa gatttttatt
     9481 tgtttaatct gcaataatac tgattggtga ttatttttgg tatatgttca ttcgattatt
     9541 ttttatgcag ctcaataaca taatcttcat caccagatga ttaatccgat ctccgctgca
     9601 acaatttcgc catattaatc gactcgacca gctgtgtccg gttgatccgg ggtgagttga
     9661 gatccatcac tttttgccat agttcgatgg cttgcgcgta attcgcctgc ataaacgcat
     9721 cggaagccag cagcatcaga gcggtgattt cgttagagtc cagcgcgagg gctttgtcga
     9781 tcattgcgcg agtctgggcg gtcatatgct ggctggcctg gtaataaagc accgtcgcca
     9841 gcgccgcata cagttcagcg ttctcaccac gtagttgcaa cgcctgacgg tacgccagca
     9901 gcgaattgct gtaatcgttt tgccacagat agtactcgcc cagtaacgcc cactgttcgc
     9961 tgttttgcgg attagcgcgg attttatcct gcaatgcctg aagctgcgct tccggggttt
    10021 gctggctggc aaactgatgt agcggatcgc gctgacgctg atactccgca cgtaccgcct
    10081 gccattttgg gcttaacaga taactgccga cacagaggaa taccatcaat atcgtgagtg
    10141 ttgtcagcat tttcaccggt atttgaggct gtttcatcgc ttcgccctca ctcgccagag
    10201 gatcagtgcc atcagcagta acaacaccac tggcagcgcc cacaacacca gcgtctgacc
    10261 cgttaacggt gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc agccaatgat
    10321 ttcgacttca cttttcccct ccgccaccat gctgtaaacc tgatggcgca tactgactgc
    10381 caccggcgcg ttggattcca gtaagttttg attctggcac tgcggacaac gtaactggct
    10441 ggcaatattt aacgcctgtt gctgttgttg cggattggcg aattgccagg tgtctacaac
    10501 ctgagcgcgc gcaaaacagg taaataacag cagtagcgtg agaagtcctt tattcatcgc
    10561 gcttcctccc ccgccatccg cttaacaatg cgcccgcaat catcaacaat cctccccccc
    10621 agatccagcg cacaccgctt tgtacataca aacgaaaagc gtaacgatcc ggcccagttt
    10681 tctcccccat gaccgcatac cagtcatgga tgccgttcca gcgaattgac ggttccatca
    10741 tttgctgacg gcgtgcttca taaaaacgcc gctccggcgt cagttcacca atacgttgct
    10801 gatggtcaaa cagtgccact atcgcttttt cgctggtgta attgcctctg gcttgcagat
    10861 cgaggcgctc aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc ggctgtaaat
    10921 tcaggctgat ttcctgacgg ctgacgctgg aaaccacgac cccagcggca aataacagca
    10981 cgcccgcatg agctaccagc gccggaagct gcacgcgttt gccagagaca aacgtcgcca
    11041 gcacaatcac caccaacatc aacagaccaa acggtaacgt cgcgcggtta aaatacggtg
    11101 cgccaacgga gaggcgtccc cagcccagca ggccgtaaat catcggataa agcgttccca
    11161 ccagcacgat cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt aacatttccc
    11221 gcgataaccc cgaaaaacgc accgccgggc caccgtcccg cgctcgccag ccatacagag
    11281 ccagagacgc aaggctaatc agtacaaaca gactgaacaa cggcacggcg cggacgttat
    11341 ccagcgcgaa cgcatgaacc gaaaccagaa tgccagaacg gacaattaag gtgcccagca
    11401 gcgacagcat cagagtaact atcgccagta acagcgacca gtggcgaaaa atcccccgct
    11461 ggcgtgtcag ggataaactg tgcagcaacg cagtggcaga aagccagggt agtaaagagg
    11521 cgttttccac cggatcccag aaccaccagc cgccccagcc cagttcgcaa taggcccacc
    11581 aggaaccgag gatgatcccc gcagttaatg cactccagcc aggtaacgcc cagcgccagc
    11641 aaattcgggc gcaggcacca tcaaactcgc cgcgcagtag actcgccagc gccacgctcg
    11701 ccgctaccat caaaccgcca tagccaaggt aaagcagcgg tggatgaaag ataagaccgg
    11761 gatgttgcag catcggattg agatcgcggc cttcgattgc tggtggaaat atccgcacga
    11821 agggatcgga ccacagcact acaaacagta gcagtgcggc gagcatgaga gataaaacgg
    11881 ctaacgtcag cggaaatagc ggatcggttt gctgccgata atgccaggca aacagcgcgc
    11941 tccaggcgga aagcagcagc acccaaagca gcagcgaacc ttcatgaccg ccccacaccg
    12001 ccgccagttt gagttcccac gacagcaggc tgtagctatg ttgggcgaca taaatgaccg
    12061 agaaatcgct gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc aggagcgcga
    12121 actgtgccag aatgccgatg caagtgagtc gcatcatggc aggccagcgc aaccgcactc
    12181 ccgcaaaggc cgtcaacggg gtcaacacgt tgaccccgag acttaacaac agcgccagaa
    12241 aaccgacttc aggaaggaat acgtccagaa accacctctg ttctggctta cgccaccgtt
    12301 aactgcccgg cgtagagaat gaaaaagcgc aacatcaaca cgccgaccag gctggctcca
    12361 cacgccgcca gcacggcagg aatgccggaa ctgcgattga cccacggttt gagcaacatt
    12421 ggcacaatca gccccagccc ggcgacacca agccagaacc accacgtcca gaaaccgcca
    12481 cccaatgccg ccaccagcgc acgcacttta ccgtcatcgc ccaaggccag accgacaaaa
    12541 aacgccacca gcaggaagat ttcaccccat accacgggga tttccatacg gtgtacaaac
    12601 tgcgcttccg tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat cagtgccacc
    12661 gccgcaccgg acgagatgcc ggagaagagg aacagcaccg gcaggatcgg gttattaagg
    12721 aacggatacg atttcagcgc cgacagcaga aaaccggtat aagcccccaa caacaccgcc
    12781 agcaccagca tcaatgtttc cagtccgcga tgaacgggcg tcagcaggct cagaaccttt
    12841 tgcacgatcc ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc atgttcaaag
    12901 attttcgcca gccacagcac cagcaccacc atgtaaagct gaaacagcat cacccccatc
    12961 gacatcaccg aggtaaaact gtagtggaac atcagcttcc agaaggtcca cggtcttgtc
    13021 aggtggaaga cgaggatcaa cagaccgagg atcaccgcgc caggtccgac aatcagcgtg
    13081 gtgcgcagca acgtgctgtc tgcaccgccc gcctgcgggt agaagcgacg taacagcaca
    13141 gccagcgtca ccaaaccagc agaaatacca atcaaaaaca ggtagatggc aatcggccag
    13201 tcccacacca gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc acctccccgt
    13261 atttaaacgg tacgcggtac agtttcggtt tggttcccag cgccagcttg tagcggtaag
    13321 taggcttctg gcgcagcagt tgcgaaatct cactgttagg atcgtccaga ttgccaaacg
    13381 tcagcgcctt cgtcgggcaa gcctcaacgc acgcgggcag cttaccggcc tgcaaattgg
    13441 tcttacggca gaaatcgcat ttgtccgccg tcttcgtgac cggatggata aagcgcacgc
    13501 ggtacggaca ggcggcgatg cagtactgac aaccgacgca gagatcagga ttaacatcga
    13561 caatcccgct agcggcatca cgaaaagacg caccggtcgg gcagacgtca acgcacggcg
    13621 catgatcgca gtgctggcaa gacttacgga agaagcgata tttcacatca ggaaattcgc
    13681 cctgcggctc gctacgaata atcgtcaggc gcgagacgcc ttccggcacc ttgttcactt
    13741 cccgacaagc atccatacag gcggtacagc cgatgcataa cgactcgtca tgcaccatgc
    13801 cgtaacgcac cccattgata ttcaacgttt tcgccaccac gcgccctgcg gtgccgctga
    13861 cggctgccag cacgccgacg ccggtgagaa actggcgacg agaccaggtc atggctgctc
    13921 cttaagcaac ggaacagacg ccgggttaaa gttcggattg gtgcgctgat cgctgtggca
    13981 atcgacgcaa atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat cttgctgtgg
    14041 atgcagggaa tggcagctgg cgcacgccac tttggttacg tggacatcgt gcggccagaa
    14101 cgctttttgc aactgttccg gcagatggca ggacatacag acgctgttct gctctccaac
    14161 cttgtacatc ggctcgttaa agcgcatcac atctttcacc ccttcgcggt gttgcggtga
    14221 tggctggccg tgacagttgg tgcaggtgac cggcagtttg ttattcgggt tgataacgga
    14281 agcatgtttg ccatgcatac cttcggtatc tggcttatga cagtccagac aggcggcatc
    14341 cgggttacgc tgctgggtaa cttcgtagcg atcatcagat gcctgcgccg caggggtagc
    14401 ggtaattccg ttcaggctcc acaacaggcc cgacgccagc accccggcag ttaataacga
    14461 acgtaatacg ctcatattca ctccattgtg ttttccgccc cttgcgaggc ggaacggggt
    14521 tattggctta acagaccgtt tttacgtgcc tgctcttccc actgcgggat caccgttttg
    14581 atgaagtcct gcttctcggc cttgatttgt tccatgttca ggccaatggc ctgctgggct
    14641 ttctctttgg tcgagatatc cgggatctgg atttcatggg tgatgccttt ggtcgccagc
    14701 aggcgcgcca gtttggtgcg tgcatccgcc gctttatcca tcgccgtacc gagcatccgt
    14761 aaaccttctt ccggtgcgtg catatgaatg ccgtgggaag cgatcgccag atcccagcgc
    14821 cactgggcat gacggatatc gtcctgaatt ggcttcattt cggcttccgt cgcgcctgca
    14881 tccagcgctg ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac cttgattttc
    14941 aggtcgttaa tcgactgctt acgttccgcg accacttttt gcaaggcagc tttgtcctgg
    15001 gtatggcagt tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat tttatggtcg
    15061 gtgtagagtt tgccttcggc gttctgcact tttggcatat ggcagtcgat acaggtcacg
    15121 ttgtttttac cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg cgctttcagc
    15181 attggcgttt tcgacaggga gttagtccag tcagagaagg caattttgtc gtaatactgc
    15241 tccatatttt cgactttcat gccgtcatcc cacgggaatt taaccgcttt gtttttgccg
    15301 tcgaagtaat actccacatg gcactgaccg caaaccatcg attgctggtc gaaacgtccg
    15361 gctttctcaa aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg acgggaaagg
    15421 gttaactccg gtttgccttt ggcgaactct ggtgaggcgg tgttatggca atcggcacaa
    15481 cctaagttgt tgacaatttc cgggccgccg cgcgcccatt taccgtggaa gtagccatct
    15541 tcgccgtctt tctggatcag acgcgccaca tccgggcttt tacaactcca gcacgccatc
    15601 ggtagcggac catcttcagc gtttttcggc gcaccggtac gcagggtttc acgcacatcg
    15661 gtcacagcaa aagcatgtcc acgcggcttg ttgtaatcgc gcgagaaggg ataccccgcc
    15721 cacaggatca ccagccgtgg atcttccgcc agggcgtcaa cacgctctga ctgttccgag
    15781 gtggctttcc aggagagata ttgatcggga tgctgcgggg caaaggtttc attcttcgct
    15841 tccacagtta caggttttgc gggagcagcc gtttgttcag cgtgaacaga agtgaaaaag
    15901 aaaaaaggaa tcaataagct gaagatacgg cgtgcgttta tttttatcct tgtcataggg
    15961 gcttcatccg aattgcgcca ttgttgcaat ggcggttttt attgtttttc acgacagtaa
    16021 ccgcacctac actgtcatga cattgctcgc ccctatgtgt aacaaataac cacactgtga
    16081 atgttgtctt taatcaattg taagtgcatg taaaatacca ctttagagtt agtcagtatc
    16141 ttcctctttt tcaacagcat gcataactgc atgttcctca aagaattaat caacttttgt
    16201 tgctgacctt caaaaattac cctgccgttt atttgcacaa ttctactttt gcgtgatctg
    16261 tcgcccaaat actaaacaaa actgccaata cccctacatt taacgcttat gccacatatt
    16321 attaacatcc tacaaggaga acaaaagcat gagtcaaatt cacaaacaca ccattcctgc
    16381 caacatcgca gaccgttgcc tgataaaccc tcagcagtac gaggcgatgt atcaacaatc
    16441 tattaacgca cctgatacct tctggggcga acagggaaaa attctcgact ggatcaaacc
    16501 gtaccagaag gtgaaaaaca cctcctttgc ccccggtaat gtgtccatta aatggtacga
    16561 ggacggcacg ctgaatctgg cggcaaactg ccttgaccgc catctgcaag aaaacggcga
    16621 tcgtaccgcc atcatctggg aaggcgacga cgccagccag agcaaacata tcagctataa
    16681 agagctgcac cgcgacgtct gccgcttcgc taataccctg ctcgagctgg gcattaaaaa
    16741 aggtgatgtg gtggcgattt atatgccgat ggtgccggaa gccgcggttg cgatgctggc
    16801 ctgcgcccgt attggcgcgg tgcattcggt aattttcggt ggcttctcgc cggaagcggt
    16861 tgccgggcgc attatcgatt ccaactcacg actggtgatc acttccgacg aaggcgtgcg
    16921 cgccgggcgt agtattccgc tgaagaaaaa cgttgatgac gcactaaaaa acccgaacgt
    16981 caccagcgta gagcatgtgg tggtactgaa gcgtactggc ggaaaaattg actggcagga
    17041 agggcgcgac ctgtggtggc acgacctggt tgagcaagcc agcgatcagc accaggcgga
    17101 agagatgaac gccgaagatc cgctgtttat tctctatacc tccggttcta ccggaaaacc
    17161 aaaaggcgta ctgcacacta ccggcggtta tctggtgtac gcggcgctga cctttaaata
    17221 tgtctttgat tatcatccgg gcgatatcta ctggtgcacc gccgatgtgg gttgggttac
    17281 cggacacagt tatttgctgt acggcccgct ggcctgcggc gcgaccacgc tgatgtttga
    17341 aggcgtaccg aactggccga cgcctgcccg tatggcacag gtggtggaca agcatcaggt
    17401 caatattctc tataccgcgc ccacggcgat tcgcgcgctg atggcggaag gcgataaagc
    17461 gatcgaaggc accgaccgtt cgtcgctgcg cattctcggt tccgtgggcg agccaattaa
    17521 cccggaagcg tgggagtggt actggaaaaa aatcggcaac gagaaatgtc cggtggtcga
    17581 tacctggtgg cagaccgaaa ccggcggttt catgatcacc ccgctgcctg gcgctaccga
    17641 gctgaaagcc ggttcggcaa cacgtccgtt cttcggcgtg caaccggcgc tggtcgataa
    17701 cgaaggtaac ccgctggaag gggccaccga aggcagtctg gtgatcaccg actcctggcc
    17761 gggtcaggcg cgtacgctgt ttggcgatca cgaacgtttt gagcagacct atttttccac
    17821 cttcaaaaat atgtatttca gcggcgacgg cgcgcgtcgc gatgaagatg gctattactg
    17881 gataaccggg cgcgtggacg acgtgctgaa cgtctctggt caccgtctgg ggacggcgga
    17941 gatagagtcg gcgctggtgg cacatccgaa gattgccgaa gccgccgtag taggtattcc
    18001 gcacaatatt aaaggtcagg cgatctacgc ctacgtcacg cttaatcacg gggaggaacc
    18061 gtcaccagaa ctgtacgcag aagtccgcaa ctgggtgcgt aaagagattg gcccgctggc
    18121 gacgccagac gtgctgcact ggaccgactc cctgcctaaa acccgctccg gcaaaattat
    18181 gcgccgtatt ctgcgcaaaa ttgcggcggg cgataccagc aacctgggcg atacctcgac
    18241 gcttgccgat cctggcgtag tcgagaagct gcttgaagag aagcaggcta tcgcgatgcc
    18301 atcgtaaccc acaattgccg gatgcggcgt ataacgcctt atccggccta cattcggcaa
    18361 gggttacccg agcgttaacc ttctcccata agggagcggg aattaaaaca atccctacat
    18421 tacctctgga gaatctgtga tgaatggtac gatttatcag cggatagaag acaatgcgca
    18481 tttcagggag ttagtcgaaa aacggcaacg gtttgccacc atcctgtcga ttattatgct
    18541 ggcagtttat atcggcttta ttttactgat cgccttcgcg cccggctggc tgggcacccc
    18601 gctgaatccg aacaccagcg tcacacgcgg tattccagtc ggtgttgggg taatagtgat
    18661 ctcctttgtt ctcactggta tctacatctg gcgggcgaac ggcgaattcg accgtcttaa
    18721 taacgaagtc ctgcatgagg tacaagcatc atgaaaagag ttctgacggc gcttgccgcc
    18781 acactccctt tcgcagctaa cgccgcggat gctattagcg gggccgtaga gcgccagcca
    18841 acgaactggc aggcgattat tatgttcctg attttcgtcg tgtttacgct cggcattacc
    18901 tactgggcgt caaaacgcgt acgttcgcgt aatgactatt acaccgcagg cggcaatatc
    18961 accggttttc agaacgggct ggcgattgcc ggtgactata tgtccgccgc ctcatttttg
    19021 gggatctccg cactggtgtt tacctccggc tatgacggct taatttactc gctgggcttc
    19081 ctggtgggct ggccgatcat tttgttcctg attgccgaac gtctgcgtaa cctggggcgt
    19141 tacacctttg ccgatgtagc ctcttatcgt ctgaaacaag ggccgattcg tattctttcg
    19201 gcctgtggtt ctctggtggt ggtggcgctt taccttatcg cccagatggt gggcgcaggt
    19261 aaactgatcg agctgctgtt tggccttaac tatcacattg cagtggtgct ggtcggcgtg
    19321 ctgatgatga tgtacgtcct gttcggcggc atgctggcga ccacttgggt acaaattatc
    19381 aaagctgtgc tgttgctgtt cggtgccagt tttatggcct ttatggtgat gaaacacgtc
    19441 ggctttagct tcaacaatct gttcagcgaa gcgatggcgg tacacccgaa aggtgtcgac
    19501 atcatgaagc cgggagggct ggtgaaagat ccgatctccg cgctgtcgct ggggcttgga
    19561 ttgatgtttg gtacggctgg cttgccgcac attctgatgc gcttctttac ggtcagcgat
    19621 gcccgcgaag cgcgtaagag cgtgttctac gctaccggat ttatgggcta cttctatatc
    19681 ctgaccttta ttatcggctt cggcgcgatc atgctggtcg gtgcaaatcc ggaatataaa
    19741 gacgcggcgg gccatctgat tggtggtaac aacatggcgg ccgttcacct ggcgaatgca
    19801 gtgggcggca acctgttcct cggttttatt tcagcggttg ctttcgccac tatcctcgcg
    19861 gtggttgcgg gtctgacgct ggcgggcgca tcagcggttt cgcatgacct gtacgctaac
    19921 gtcttcaaaa aaggcgcgac cgaacgtgaa gagctgcggg tatcgaaaat caccgtcctg
    19981 attttgggtg tgattgcgat tatcctcggc gtgctgtttg agaatcagaa catcgccttt
    20041 atggtggggc tggcgtttgc catcgcggcg agctgtaact tcccgatcat tctgctttct
    20101 atgtactggt cgaaactgac tacgcgtggc gcaatgatgg gctgctggct ggggctaatt
    20161 accgcagtgg tactgatgat cctcggcccg acgatttggg tacagatcct cggtcacgaa
    20221 aaagccatct tcccgtatga atacccggcg ctgttctcta tcagcgtggc attcctcggc
    20281 atctggttct tctcggcaac cgataactca gcggaaggtg cgcgcgagcg tgaactgttc
    20341 cgtgcgcagt ttatccgctc ccagaccggc tttggcgttg agcaaggccg cgcgcattaa
    20401 tctctcccct t
//
LOCUS       DAZGBC010000086        20366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGBC010000086 DAZGBC010000000
VERSION     DAZGBC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.086"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..369
                     /locus_tag="ACHK1J_002989"
     CDS             <1..369
                     /locus_tag="ACHK1J_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7912232.1"
                     /translation="PAGPQGPKGEKGDPGPRGERGETGPMGPPGASGDDGKSRVMGFR
                     LGNPVTYPLQEGENGWSVDLADGSVITGLSGNTQGGVSRVTTIYYRPLQMTFDGSHWR
                     TVGVGEYVSSGASGREYFPL"
     gene            411..1082
                     /locus_tag="ACHK1J_002990"
     CDS             411..1082
                     /locus_tag="ACHK1J_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO7912233.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVNQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            complement(1522..1851)
                     /locus_tag="ACHK1J_002991"
     CDS             complement(1522..1851)
                     /locus_tag="ACHK1J_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7912234.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(1992..2079)
                     /locus_tag="ACHK1J_002992"
     tRNA            complement(1992..2079)
                     /locus_tag="ACHK1J_002992"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2043..2045),aa:Ser,seq:tga)
     gene            2505..3623
                     /gene="hyaA"
                     /locus_tag="ACHK1J_002993"
     CDS             2505..3623
                     /gene="hyaA"
                     /locus_tag="ACHK1J_002993"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO7912235.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            3620..5413
                     /gene="hyaB"
                     /locus_tag="ACHK1J_002994"
     CDS             3620..5413
                     /gene="hyaB"
                     /locus_tag="ACHK1J_002994"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO7912236.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            5432..6139
                     /gene="hyaC"
                     /locus_tag="ACHK1J_002995"
     CDS             5432..6139
                     /gene="hyaC"
                     /locus_tag="ACHK1J_002995"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO7912237.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            6136..6723
                     /gene="hyaD"
                     /locus_tag="ACHK1J_002996"
     CDS             6136..6723
                     /gene="hyaD"
                     /locus_tag="ACHK1J_002996"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO7912238.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLGAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            6720..7118
                     /gene="hyaE"
                     /locus_tag="ACHK1J_002997"
     CDS             6720..7118
                     /gene="hyaE"
                     /locus_tag="ACHK1J_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO7912239.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            7115..7972
                     /locus_tag="ACHK1J_002998"
     CDS             7115..7972
                     /locus_tag="ACHK1J_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO7912240.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            8106..9650
                     /gene="appC"
                     /locus_tag="ACHK1J_002999"
     CDS             8106..9650
                     /gene="appC"
                     /locus_tag="ACHK1J_002999"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO7912241.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQSFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            9662..10798
                     /gene="appB"
                     /locus_tag="ACHK1J_003000"
     CDS             9662..10798
                     /gene="appB"
                     /locus_tag="ACHK1J_003000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7912242.1"
                     /translation="MFDYETLRFIWWLLIGVILVAFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10811..10903
                     /gene="cbdX"
                     /locus_tag="ACHK1J_003001"
     CDS             10811..10903
                     /gene="cbdX"
                     /locus_tag="ACHK1J_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO7912243.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10983..12287
                     /gene="appA"
                     /locus_tag="ACHK1J_003002"
     CDS             10983..12287
                     /gene="appA"
                     /locus_tag="ACHK1J_003002"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO7912244.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCP
                     QSGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(12407..14573)
                     /gene="etk"
                     /locus_tag="ACHK1J_003003"
                     /pseudo
     CDS             complement(12407..14573)
                     /gene="etk"
                     /locus_tag="ACHK1J_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(14593..15039)
                     /gene="etp"
                     /locus_tag="ACHK1J_003004"
                     /gene_synonym="yccY"
     CDS             complement(14593..15039)
                     /gene="etp"
                     /locus_tag="ACHK1J_003004"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO7912245.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(15027..16166)
                     /locus_tag="ACHK1J_003005"
     CDS             complement(15027..16166)
                     /locus_tag="ACHK1J_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO7912246.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTAAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(16212..18308)
                     /locus_tag="ACHK1J_003006"
     CDS             complement(16212..18308)
                     /locus_tag="ACHK1J_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7912247.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(18308..19054)
                     /locus_tag="ACHK1J_003007"
     CDS             complement(18308..19054)
                     /locus_tag="ACHK1J_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7912248.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLAVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(19051..19695)
                     /gene="gfcB"
                     /locus_tag="ACHK1J_003008"
     CDS             complement(19051..19695)
                     /gene="gfcB"
                     /locus_tag="ACHK1J_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO7912249.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19802..20107)
                     /locus_tag="ACHK1J_003009"
     CDS             complement(19802..20107)
                     /locus_tag="ACHK1J_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912250.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         4759 a         5180 c         5494 g         4933 t
ORIGIN      
        1 cccgctggtc cgcagggacc gaaaggggag aaaggcgacc ccggtcccag aggtgagcgt
       61 ggagaaaccg ggccgatggg gccgccgggg gcatcaggag atgacgggaa gagtcgtgtc
      121 atgggcttca ggctggggaa tccggtaacg tatccgttgc aggagggtga aaatggctgg
      181 tcagttgatc tggcagatgg ttcagtcatt acagggcttt cggggaacac tcagggcggc
      241 gtcagccgtg ttacaacgat ttactacagg ccgttgcaga tgacgtttga tggctctcac
      301 tggcgcaccg ttggtgttgg tgagtatgtg tcgtcaggag cttcggggcg tgagtatttt
      361 ccattataaa gcgcatcatc tttcagacat gtaaccggga ggacttacag atgcacataa
      421 agaatttcag acaatacacg ccggaaaatc cggatgtgcc gggtgcgatg tacctgaaat
      481 cagaggatgg tcaggactgg tatgagtgtc agtcgttatt ttcagcagag acgctgaagg
      541 tggtttataa cagtgccggg gtcattaccg gcatcagcag ggtggcgtca gttctgtggc
      601 ctgtgaacca gagtgtggtg gaggtggcgg atacggaaga aaaccgcaaa gctgacatct
      661 cggggcgctg gggttttgac ggggagaaaa tcacggacct gctgaccgcg gagaaagcgc
      721 gagggatgaa gggcgatgaa attaacgcct ggcgtaatgc gatggaagcg gcgaactaca
      781 cgtttgagca caatgggcgg aaatgggact acgggaagtc aacgcagacg cgtcttgagc
      841 cgtcggtggc ggcagcgaaa gcggggaaac tgccggaggc gtttttctgg acggatgcgg
      901 aaaacaatga tgtgccggtg acagcagaag agcttattgc gctgagtgaa gcggcagagc
      961 aggcgatgtt caccaaaggg atggaaatcc acgtccggca gcgcaccatg aagaaagagc
     1021 tggaaaagct gaccagtgcg gatgagatac tggcatacag ggttggctgg gcacaggaat
     1081 aacagacaaa aagatggggg accatcacct cccccatgaa ctgagtcgat tatgtaatta
     1141 ttgtgaagag accttaccta cacagataaa gagtgtcacg gaaaagttaa tcctgaaagt
     1201 gatgcaggtc tcatttccag agcatcctgt tgatatggat aatttttcag atttccggaa
     1261 gtgagagttt ttggtgtttc cataccagcc acaattgatg tgttttatgg cgcctggcag
     1321 tgcgattaag ggaacagggg gtaataaaaa aaacgggggt atcaccacac ccccggtaca
     1381 agaaaggagt aactataatg gaaaaattaa tgaagacctt tagtgctacg ttatatggca
     1441 aataaagact gccacaggga agtttgcaga aacagtgatg ccgatcgtgt tcctgtgctc
     1501 ttctctgata agagtaattt ttcagatttc cggaagtccg tattttgccc gggcgcggtt
     1561 gcatgcttca ttcgcgatgc cattttctcc ggacattgcg aattcccggc atgtggacgg
     1621 acggtttttg taaacagagc aatatgcgtt tttacccgga gttcccgcca gagcgacaca
     1681 tcggggattt ttctgactgg tgccgcgcat acagcgatga aagggggata tttgctcagt
     1741 gagactgacc ggaacctttc cgccagcatc gtcagcttct gcccagtaaa aagagacgcg
     1801 gaaaaatgca caacaggcac cacacgtcat gcatggattg gggttattca taattcatcg
     1861 gtatagcgat aaatcgtaaa gagaacaatg tacctagtgt gtattgagct aaatatacca
     1921 attagtatac caagtgtata ctaagagcaa aaaaaggaaa ggggctacca tttggtaacc
     1981 ccttgattgt ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt
     2041 tttcaagacc ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac
     2101 atcccgatgc ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg
     2161 cattgatagc gtgaaataaa agcagattga tcatttcatc accataaatt ccttctcttt
     2221 tactcgttta gcaactggct aaacatccca ccgcccggca aaaagaaaaa taggtccatt
     2281 tttatcgcta aaagataaat ccacacagtt tgtattgttt tgtgcaaaag tttcactacg
     2341 ctttattaac aatactttct ggcgacgtgc gccagtgcag aaggatgagc tttcgttttc
     2401 agcatctcac gtgaagcgat ggtttgcctt gctacaggga cgtcgcttgc cgaccataag
     2461 cgcccggtgt cctgccggtg tcgcaaggag aagagacgtg cgatatgaat aacgaggaaa
     2521 cattttacca ggccatgcgg cgtcagggcg ttacccggcg cagctttctc aaatattgta
     2581 gtctggctgc cacgtcgctg ggattaggcg cgggaatggc accaaagatt gcctgggcgc
     2641 tggagaacaa accgcgcatt ccggtagtat ggatccacgg tctggaatgc acctgctgta
     2701 ccgaatcttt tatccgctcc gctcacccgc tggcaaagga cgtcatcctt tccctgattt
     2761 ccctcgatta cgacgatact ttgatggctg ccgccggaac ccaggcggaa gaagtatttg
     2821 aagacatcat cacgcaatac aatggcaaat atatcctcgc agtagaaggt aatccgccgc
     2881 tgggcgagca ggggatgttc tgtatcagca gcggtcgacc gtttattgag aaactcaaac
     2941 gtgccgctgc cggagccagc gcgattatcg cctggggaac ctgcgcgtcc tggggctgcg
     3001 tgcaggccgc gcgacctaat ccgacacagg caacgcctat cgacaaagtc atcaccgaca
     3061 aacccattat caaagtacct ggctgcccgc cgatcccgga tgtgatgagc gccatcatta
     3121 cttacatggt gacctttgat cgcttgccag atgtcgacag aatgggtcgt ccactgatgt
     3181 tttatggtca gcgtatccac gataaatgct atcgccgcgc tcacttcgac gccggagagt
     3241 tcgtccagag ttgggatgat gacgctgccc gcaaaggtta ttgcctgtac aaaatgggct
     3301 gcaaagggcc taccacctat aacgcctgtt cctccacacg ctggaatgat ggcgtttctt
     3361 tcccaatcca gtctggtcac ggctgcctgg gctgtgcgga aaatggtttc tgggatcgcg
     3421 gttcgttcta cagccgcgtg gtcgatattc cgcaaatggg tactcattcc accgccgata
     3481 ccgtcggttt aaccgcgctt ggcgtggtgg cagcggctgt tggtgtgcac gcagtcgcca
     3541 gcgccgttga ccagcgcaga cgtcataacc agcaacctac agaaaccgaa catcagccag
     3601 gcaatgagga taaacaggca tgagcactca gtacgaaact cagggataca ccatcaataa
     3661 tgccggacgc cgcctggtgg tcgacccgat tacgcgcatt gaaggccaca tgcgctgcga
     3721 agtgaatatt aacgatcaga atgtgatcac caatgccgtc tcctgcggca ccatgtttcg
     3781 cgggctggag atcatcctgc aagggcgcga cccgcgcgat gcgtgggcgt tcgttgaacg
     3841 tatctgcggc gtctgtactg gcgtacacgc cctggcttcg gtttacgcca tcgaagatgc
     3901 aatcggtatt aaagtgccgg acaacgccaa tatcatccgc aacattatgc tggcaacgct
     3961 ctggtgccac gatcatctgg tgcacttcta tcagctcgcc gggatggact ggatcgatgt
     4021 gttagatgcg ctgaaagccg acccgcggaa aacctccgaa ctggcgcaaa gtctctcctc
     4081 atggccgaaa tcatcccctg gctatttctt cgacgtacaa aatcgcctga agaaattcgt
     4141 tgaaggcggg cagttgggga tcttccgcaa tggctactgg gggcacccgc agtacaaact
     4201 gccgccagaa gccaacctga tgggctttgc ccactatctc gaagctctcg atttccagcg
     4261 tgaaattgtc aaaatccacg cggtctttgg cggtaaaaac ccgcatccaa actggattgt
     4321 cggcgggatg ccatgcgcca ttaacattga cgaaagcggc gcggtcgggg cggtcaatat
     4381 ggaacgcctg aacctggtgc agtcgattat cacccgcacg gcagacttca ttaacaacgt
     4441 gatgatcccc gacgccttag ccatcggtca gttcaacaaa ccgtggagcg aaatcggcac
     4501 ggggctttct gataaatgtg tcctcagcta cggcgcattc ccggatattg ccaacgactt
     4561 tggtgagaaa agtctgctga tgcctggcgg cgcggtgatt aacggcgact tcaacaatgt
     4621 gctgccagtg gatttggttg atccgcagca ggtgcaggag tttgtcgatc acgcctggta
     4681 tcgttatccc aacgatcagg tcgggcgtca tccattcgat ggcattaccg acccgtggta
     4741 caaccccggc gatgtcaaag gcagcgatac caacattcag cagctgaatg aacaggaacg
     4801 ctactcgtgg atcaaagcgc cgcgctggcg cggtaacgcg atggaagtgg ggccgctggc
     4861 acgcacgtta atcgcttatc acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat
     4921 gatgtcggcg ctgaacctgc cgctttccgg tatccagtca acgttgggcc gcattttgtg
     4981 ccgcgcgcac gaagcacagt gggccgcagg taagttgcag tatttcttca acaagctgat
     5041 gactaacctg aaaaacggca atcttgccac cgcctccacg gaaaaatggg aacccacaac
     5101 ctggccgaca gagtgccgtg gtgtcggctt taccgaagcg ccgcgcgggg cgttaggcca
     5161 ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg
     5221 gaacgccagc ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa
     5281 caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga
     5341 tccgtgcctc gcctgttcaa cacacgtgct gggcgacgac ggtagcgagc tgatctccgt
     5401 gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag
     5461 ccactatgtc tttgaagcgc cagtgcgtat ctggcactgg ctgacggtgt tatgcatggc
     5521 ggtgttgatg gtcaccggtt actttatcgg caaaccgcta ccttccgtca gcggcgaggc
     5581 gacgtatctg ttctatatgg gctacatcag gttaattcac ttcagcgccg ggatggtttt
     5641 taccgtggtc ttgctgatgc ggatctactg ggcttttgtt ggcaatcgat actcccgcga
     5701 gctgtttatc gtgccagtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg
     5761 ctggtatctg tttctggcaa aacgtccgag tgccgatata ggccataacc ccatcgccca
     5821 ggcggcgatg tttggctatt tcctgatgtc ggtctttatg atcatcaccg gctttgcgct
     5881 gtacagcgaa cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt
     5941 ctactggacg ggaggcaact caatggacat tcacagctgg catcggctgg ggatgtggct
     6001 gattggcgcg tttgtgatcg gtcatgtcta tatggcgctg cgtgaagaca tcatgtccga
     6061 cgacacggtg atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag
     6121 taacaaggag cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg
     6181 tgggccgatg aaggtttcgg cgtgcgggtg gcggaacggt tgtatgccca ttaccactgg
     6241 cccgagtatg tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc
     6301 gaaagcgcca gccatctgtt gattctcgat gccattgact acgggctgga acctggaacg
     6361 ctgcgaacct atgccggaga acgtattccg gcttatctca gcgcgaagaa aatgagcctg
     6421 caccagaaca gtttctccga agtgctggcg ctggcagata tccgcggaca tctgccagca
     6481 catattgccc tcgtcggtct gcaacccgca atgctcgacg actacggcgg cagcctgagc
     6541 gaactggcac gggagcaact gcccgcagcg gaacaggcgg cgctggcgca gctgggtgca
     6601 tggggaattg tgccgcaacc ggctaatgaa tcgcgctgtc tcaattatga ctgtctgtcg
     6661 atggaaaatt acgaaggcgt tcgcttgcgc cagtacagga tgacactgga ggagcaggga
     6721 tgagcaacga cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc
     6781 cggttagtga attccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat
     6841 taagcagtga cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat
     6901 tactgcgcga gtttcccgac tacacatggc aggtggcgat tgctgacctt gaacagagcg
     6961 aagccatcgg agatcgcttt ggcgtctttc gctttcccgc cacattagtg tttaccggcg
     7021 gaaactatcg cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc
     7081 gtgggcttgt cgaaccacaa caggagcgtg cctcatgagc gaaacttttt tccatctgct
     7141 ggggccagga acgcaaccga acgatgacag tttcagcatg aatccgctgc cgatcacctg
     7201 tcaggtgaat gatgaaccga gtatggcggc cctggaacag tgtgctcaca gcccgcaggt
     7261 gattgcgctg ttaaacgagt tacaacatca actaagcgaa cgccagccgc cgttgggcga
     7321 ggtgctggca gtcgatctgt taaatctcaa tgccgacgat cgtcacttta tcaatacgct
     7381 tctcggggaa ggggaagtgt cagtgcgcat tcagcaggct gacgacagcg aaagtgaaat
     7441 acaggaggcg atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct
     7501 ggaggacaaa ctggaagctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa
     7561 tctcttgccg acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc
     7621 gttggcgcat gagttactgg cgcatgtacg taaccccgac gcgcagccgc acagcattaa
     7681 tctgacgcaa ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc
     7741 gggaaatatt cagattcgta ccattggcta tggcgagagc tatatcaacg ccacggggtt
     7801 acgccatgtc tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta
     7861 tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc
     7921 gcagcggctt agcgaggtat gtcagtggct ggcggaaggt gcaccgacgt aaaaagacgg
     7981 taagtatcgc tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt
     8041 tcaggataaa gagggagatc taccattatc gggttatttt tctctcttcg cctacaggag
     8101 tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc
     8161 actttttatt tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca
     8221 tttacgtggt caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct
     8281 tcggtatcaa ctttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta
     8341 ctaactggtc attctattcc aactatgtgg gcgatatttt tggcgcaccg ctggcgatgg
     8401 aagcattaat ggccttcttc ctcgaatcca cctttgtcgg gcttttcttc ttcggctggc
     8461 agcggctgaa taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc
     8521 tctctgcatt gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt
     8581 ttgatatcga caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg
     8641 tcagccaggt gaaatttgtg cacaccgtaa tggcgggcta cgtgaccggg gccatgttta
     8701 ttatggcgat cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgct
     8761 cgtttgccat cggttccgtc ttcggtactc tggcgattat tggcacgctg caactcggag
     8821 acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg
     8881 agtggcaaac ggaacctgca cctgcaccgt tccatgtggt tgcatggccg gaacaggatc
     8941 aagagcgtaa cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact
     9001 cattagataa acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc
     9061 aacgcggacg tatggcctgg ctgttaatgc aggaaatatc gcaaggcaat cgtgagccgc
     9121 atgtgttgca gtcattccgg gagctggaag gcgacctggg ctacggcatg ttgctctccc
     9181 gctatgcgcc ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg
     9241 cgatacctca ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc
     9301 tgctgctact ggtgatgctt attgcgcttg tccagacgct gcgtggcaaa atcgaccagc
     9361 atcgctgggt gctgaaaatg gcgctctgga gtttgccgct gccgtggatt gcgattgaag
     9421 ccgggtggtt tatgaccgag tttggtcggc agccgtgggc gatacaggac atcttaccga
     9481 catactccgc gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg
     9541 taggccttta caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc
     9601 tggggccgag cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat
     9661 catgtttgat tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt
     9721 ggcctttatg atctccgacg gatttgacat ggggattggc tgtttgctgc cgctggtggc
     9781 gcgtgatgat gatgaacgcc ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa
     9841 tcaggtctgg ttgatcctcg caggtggggc attatttgcc gcctggccca gagtgtatgc
     9901 agcggcgttt tccggctttt atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg
     9961 cccgctggcc tttgattatc gcggaaaaat cgccgatgca cgctggcgta aaatgtggga
    10021 tgccggtctg gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa
    10081 cttgttgctc ggcgtgccgt ttgccttcac tccgcaatta cgcgtggagt atctcggcag
    10141 cttctggcaa ctgctgacgc cattcccttt attgtgcgga ttgctcagcc ttgggatggt
    10201 gattttgcaa ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc
    10261 acagctggcg actaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcgggtta
    10321 ctggctgtgg gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc
    10381 caatccgtta atgaaactgg tggcagtgct acctggtgcc tggatgaata attttgtcga
    10441 gtcgcccgtt ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat
    10501 ggcgatttat cgtggtcgcc cgggttgggg atttttgatg gcgtcattga tgcaatttgg
    10561 cgtgattttc acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gcgtgagtcc
    10621 aatctccagc ctgacgttgt gggacagtac ttccagtcag ctgacgctga gcattatgtt
    10681 ggtaatcgtg ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa
    10741 aatgtgggga cgcatgacga cagaaactct ccgccgtaac gaaaacgagc tgtactaagg
    10801 agcaggaaca atgtggtatt tactttggtt cgtcggcatt ttgttgatgt gttcgctctc
    10861 cacccttgtg ttggtatggc tggacccgcg attgaaaagt taacgaacgt aagtctgatg
    10921 cggcgcatta gcgtcgtatc aggcaatcaa taatgtcgga tatgaaaagc ggaaacatat
    10981 cgatgaaagc gatcttgatc ccatttttat ctcttctgat tccgttaacc ccgcaatctg
    11041 cattcgctca gagtgagccg gagccggagc tgaagctgga aagtgtggtg attgtcagtc
    11101 gtcatggtgt gcgtgcccca accaaggcca cgcaactgat gcaggatgtc accccagacg
    11161 catggccaac ctggccggta aaactgggtt ggctgacacc gcgcggtggt gagctaatcg
    11221 cctatctcgg acattaccaa cgccagcgtc tggtagccga cggattgctg gcgaaaaagg
    11281 gctgcccgca gtctggtcag gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa
    11341 caggcgaagc cttcgccgcc gggctggcac ctgactgtgc aataaccgta catacccagg
    11401 cagatacgtc cagtcccgat ccgttattta atcctctaaa aactggcgtt tgccaactgg
    11461 ataacgcgaa cgtgactgac gcgatcctca gcagggcagg agggtcaatt gctgacttta
    11521 ccgggcatcg gcaaacggcg tttcgcgaac tggaacgggt gcttaatttc ccgcaatcaa
    11581 acttgtgcct taatcgtgag aaacaggacg aaagctgttc attaacgcag gcattaccat
    11641 cggaactcaa ggtgagcgcc gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa
    11701 tgctgacgga aatatttctc ctgcaacaag cacagggaat gccggagccg gggtggggaa
    11761 ggatcactga ttcacaccag tggaacacgt tgctaagttt gcataacgcg caattttatt
    11821 tactacaacg cacgccagag gttgcccgca gccgcgccac cccgttgttg gatttgatca
    11881 tgacagcgtt gacgccccat ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt
    11941 cagtgctgtt tatcgccgga cacgatacta atctggcaaa tctcggcggc gcactggagc
    12001 tcaactggac gcttccaggt cagccggata acacgccgcc aggtggtgaa ctggtgtttg
    12061 aacgctggcg tcggctaagc gataacagcc agtggattca ggtttcgctg gtcttccaga
    12121 ctttacagca gatgcgtgat aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga
    12181 aactgaccct ggcaggatgt gaagagcgaa atgcgcaggg catgtgttcg ttggccggtt
    12241 ttacgcaaat cgtgaatgaa gcacgcatac cggcgtgcag tttgtaatgt ataaaaaaga
    12301 gcattcagct acctgaatgc tctgaggctg atgacaaacg aagaactgtc taatgcgtag
    12361 accggaaaag gcgttcacgc cgcatccggt cactttacct tcagttttac tctttctcgg
    12421 agtaactata accgtaatag ttatagccgt aactgtaagc ggtgctggcg cgtttaatca
    12481 caccattgag gatagcgcct ttaatattga cgcctgcctg ttccagacgc tgcattgaca
    12541 aactcacctc tttggcggtg ttcaggccaa aacgtgcaac cagcaggctg gtgccgacag
    12601 agcgccccac gaccgcagca tcgctcaccg ccagcatcgg cggcgtatcg acaatcacca
    12661 gatcgtaatg gtcgttcgcc cattccagta attgacgcat ccgatcgcgc atcagcagtt
    12721 cagacgggtt aggtggcacc tgaccgcgag taatcacatc aaagcctcct ttgccaaaat
    12781 gctggatcac tttgttgagc tcatctttac ctgccagata ttccgacaag ccatgttcat
    12841 tactcacggt aaacaggtta tgcgaataac cacggcgtaa gtcggcatca ataaataaca
    12901 ctttttgatc ggactgggcg atcaccgctg ccagagttga actgacaaat gttttaccac
    12961 tgtctggcgt cgcaccggtg atcatcagaa tgttattctc agtctccatc atagcgaaat
    13021 gcagactggt tcgtagcgca cgtacggctt ccacagcaga atccgccggg ttatccaccg
    13081 ccaggaaggg gatattttta gtacgatggc gctgctgatt agaaaataaa tttttcttac
    13141 gcagacgggt gcgtttatcc agccactcgg acattgggat agtggcataa acgctgatgc
    13201 cgtgctcttc cagttgttcc ggtgcttcta caccacgacg caacatcgca cgcgccagca
    13261 cggcacccac cgaaataaac aggccaagaa taaaaccaag caccacgttc aacgcttttt
    13321 tcggtttcac tggctgcggc tgagtgactg ccgggtcgat aatccgcacg ttaccaatgg
    13381 cactggattt cgaaatactc aactcctgct ggcggttaag taattgcaga tataccgcac
    13441 ggcccgcttc tacgtcacga cttaaacgca acacttcctg ttgggtggaa ggcatagccg
    13501 atacccgctt attcaggcgt ttgcgttctt gctccagcgt ctggcgtttt tccagcagcg
    13561 cacgataagt tgggtgatct ttcttataca gctgggagat ctctgcctcg cggaaggtca
    13621 gctcattgag ttgattatca acgttcacaa tctgctcaag aacgactttg gcttccaggt
    13681 taaggtcaac cgaatcgcgc tgctggcgat aaacgttgag tttttcttcc gcctggtcca
    13741 gctcgctgcg cacttcaggc aactggcgct gtaagaattc aaggctttgt gaatcctgcg
    13801 ccgcctggcg agcgatattc tgttgcaaat agttgttagc gatgctgttc agaatacggg
    13861 taatcaactg gggatcatca ccggtcatgg taagttccag catcccgctt tctttactgc
    13921 gttcgctaac ggtaaaggtt tcctgcaacg cgttaatcgc ttccagttcg gtacgttggc
    13981 tcaggacaaa ctgtgttcct ggtttggcct taatatccgc tatagtcagc gcaacgccat
    14041 ctttttccag acgctggccg accataccat tgacggtgaa ctcttcacct tccagcgtat
    14101 agtggccgtt ttccccaacc gtgagtgtca gttgctgatc ctgaccattc agttgtggaa
    14161 tatgcatcca gctgatcgcc agctcacctg gtttctcttt ggttaatctc gcccagccgc
    14221 gacccacaat cggaaaatac ttctgctcaa ccatgtcgcg cagattcagt tcagcaatgg
    14281 ttttaccgag aatcatgcgc gattgcagca gttggatctc cggtgcagac tcgggcgatg
    14341 agttagggat catatcactc aggccgctga gaatggcgtt gccctgtttt tgctcaacct
    14401 ggaccagagt atctgcctga taaattggtg tgcttaacag cgatcagcgt gaataacgcg
    14461 gtcacgctga taataaactt acggtgatcc cataactcgc cgaccagacg aagcagatcg
    14521 atctcatttt cctgagtgct gcctggtggc gtattcatat ttttagttgt catcgtattt
    14581 tataaactca aattaccggc tgaggcgctt cgcccattcc tgactggcgc gctccaacat
    14641 accgtagaca tgttcaaatg cgtcctgact tttacgatag ggatccggga tctctttctg
    14701 ttccagccat tgcccaaaca gcattgtctt cccgcggacc tcgggagcga ttgcggtaac
    14761 ctgagcaata tgttccgact ccattgccag aatcagatcg taatttcgtg ccatctcagc
    14821 agtgagctta cgtccggcat gtccttcaag agatacgcca tgattagcgg caacatctgc
    14881 cgctgtcgca tctgcagggt gttttaccag accatgaaca cctgccgatt tgacttttac
    14941 gcccggcaga cgtttacgca gcaagcgctc gccaatgggc gaacggcaaa tgttgccggt
    15001 acaaaccacc aggattgagt taaattttag ttgggccatg tttgaatcca ccgtgaagtc
    15061 tctgtcaggt catgcacccc ggaaatcgtc ggtaccagca gagaaatcac acggttccaa
    15121 cgagccagag gcgcggtagt gacataaaca atgtcgtaag gttcaagctg gaattccgtt
    15181 ccgagtatca ttgccgatgc gtccttcgca ttcagctgat aaatattggc gattttgcca
    15241 ttctgcttgt tttgcgttgc acgaataaca aaaatccccg tcgcatcagc gacatcctga
    15301 ttcataccct cagcgttacc cagcgcttcc gccagggtca taccgctgcg gtccattttc
    15361 agcgtgctct gtttacccac ttcacccatc acgaagactt tcagatcatc gttgcgcgga
    15421 ataaataaaa tatctcccgg gtgcagcagt ttgttctggc gcaagtcgcc acgctgcatc
    15481 agcgcataga ggttcacttt ggtctttacg ccattctggg tgagcaccac attgcgccag
    15541 tctgcatctg cggtcagacc gcctgcggcg ttaatcgcat ccatgatggt cagaggaata
    15601 ttggtaatcg gctgctgacc cgatttgctg acttcgccgg tgacgtacgc tttttgcgat
    15661 cggaacgccg cgacgctgac atcgacctgc gggctttcaa tgactgaatc cagacgcgct
    15721 gtaatctcgt tgcgtacctg ggtaagtgtt ttcccggcca ctttcaggcg accaatatag
    15781 gggtagaaaa tcgcaccatc ggcgttaacc cagttgccgg tatcactggc gctacggtat
    15841 tgccctgcgg cggtggttag ttccgggtga tcccagaccg taaccatcaa cacatcacca
    15901 atgccgatac gatattcata gttcgcgatt tgctgctcca gctccgggtt agcctgcgac
    15961 ataatcggtt tggcgcgcaa ctgatcaatc aatcctggcg tgacgggata gatattgacc
    16021 attttgtcca ggtcatactg attgtcaggt agttcaatga catccttgtt actggtcgat
    16081 aaattctgac caggaacaag ggtacatgct gtcaaagaca gaacagccaa tgtcagtacc
    16141 gaaaacttaa aaatgttctt tttcattggt ttatatttat cagtcaatgt aatgttatga
    16201 attacgtcag gttattgtcc aaccggtact tctcgctctg ccgtcattgg atagagctgg
    16261 tatttgcgtg acagcatttg tccaccatca cgcgtcaatg gtgtccacga aaccaccgcg
    16321 cggttgcggt taggtccaat ggtcatcaag tcgaacggaa tcgagatata aaaaccttta
    16381 ctaaagccgc cttcgccgta gtcatcttta gatacgttac tgattgccgc ccatacccct
    16441 accgccacgc cgctgtcaaa gcgtttggcg acgtcgatcg ttgcgccttt atcttttgcc
    16501 agatattgcc caacgctaag tttcatcagt acgccattga gcgtcggcgg gttccagtaa
    16561 gccgtcacga aacctgtggg cgtggaataa tcggtgaagc gcatcatgtt gtcccagtca
    16621 cgttgcttca cgtagttaac gtccacaccc agcgcccagc tggcatctag cgggcgataa
    16681 agcagctcgg aaccgacacc ggcgtacatc gtttccagat aaccgccata cacctggcca
    16741 tagaaaccat tgcctaagtc ggcaaagtag ttcgcctgca agttgttgag ataaacgtca
    16801 ttgcgaacgt agtcacggat atgcgtgcgc acgcggggca gggtagagtc cgcgggcaac
    16861 agtgaagact taaacttgtc gtagttgttg taaatattgg tgaaaatacc gccatcaagc
    16921 agcaggtggt cggtaaacca gtagcgggcg ctggacatca gccccagctg gaacatatag
    16981 aaatcttccg ggccgcccag cgactgtgaa agtgttgggt tgaaagagta gctaaagcga
    17041 tcttcacgaa tacggtagcc ccgaccaaag gcagaaagat cttcagcttc aacacgttgt
    17101 tgttgcagtg gctctgattg accaggcgct gtacctgcca gctgtttgcg caggctggct
    17161 acgtcggttt cggtagtcac catcgccata tgctcgcgct tttgcgtcac gctaatcttc
    17221 tcaacgcctt gcggcaggtt attcaccaga atccggttgg cacgatcgac cgcttcacga
    17281 gagtcacggt atttgtattg ctgaccagac atatacagcg tcttatcgcg tagctgaatt
    17341 tctggcgcgt caaagcccgc gttatacttc agggcggtaa gttggtttgc taccgtggtg
    17401 tactgcaatc cttcagattc aggcgcaggt tgatatgccg gttttggcgt atcgcgcagg
    17461 gcaggacgca gatcgttgaa attggtccgt aaagtgaaac caaacatcaa cgtgttaccg
    17521 cgttcataac tcaggttgag gtctgcccag ctggcagcgc gataaactgc accgacgttg
    17581 aaatggcttg cctgaggcag tttgccagcg aaatcattct ggtaattgtt gccgtcgtat
    17641 tcgagtttca gacgcagggg attccacggc gtttgatact caatgccgcc aaagatggaa
    17701 gccgggccac gaaagatatc gctaaagctg atatcgcccg catcgtgaga ctctgcgcga
    17761 tgacaatatt tatcgctcac ccggcaaaac gggttggtaa tattgcccgc attaccggcg
    17821 tagccccatg ccatcccgag ggtgaaatca aatggccccg cttgcttgct ggccaccaaa
    17881 tactcaccgt caaacagacc cgtaccagca atatcacgtt taccaaacgc cacttgcggt
    17941 agccagtaac cttcttccca caggcgaagt ttaaaatcga atgatttgtc tttgtatgac
    18001 tgatcgccgc tgaaatcttc ccactggcta tatttacgtg tgcgcacatc cgtataacga
    18061 atggtgcctt ccagccatgg gaacagcgcc acggaggtgg agtagaaacg gtattgatcg
    18121 ttatcccggt agttgacgct gaattcacct tccggtgcga tgcgcgcatt tggcatctgc
    18181 aacaatcctg tgccgccaaa gtccgattgc gacgaaccca gcggatccgg gtaggttaat
    18241 acttcagcat gacaggcact ggagacggca atggccaggc agcttaaaag ataagaattc
    18301 ttcttcatca gtcaggaaca cgttgcgtta aaacagacac aatctggtca tttaaatcag
    18361 catatttttc gggcagtaca tgcgcagaaa agcctaacca aagctggctg ccaggcggtg
    18421 gttccacatg tcttttattc cacagggcaa caggggcgac gacagtctca ccctcaggcg
    18481 taatcacgat aacgttattc ttgtccgcgc ctgcaagacg ggggtgatct tgcagataat
    18541 cggtaacgct acggcctgcc tgccagggca attgcccagc cccagaaacc gcacccagca
    18601 gagtaatagt caccggacgc tgtacggtat agagcgtata gtccccgacc agcgggggat
    18661 tgctgttctc atccactcgt acaaaatcag ggtccagttt gaccgctaag cgaccagtga
    18721 tattgagatt gagtaactgc tggcgcacgg atttaatcgt tgccgccaca tcgtcgtcag
    18781 cttctgcttc ccaggaagca agctgtgcca tgacatgttg gtagtcttta agcgctttag
    18841 ctttcgccgc agagtcggtc agcaaggcac caggccacca gaggcgatcg cgcagttgcg
    18901 gttgtgtcac caactgggca acattttcca cggggccaac ggaaagtgtc tgttgttcgc
    18961 caggcagata aatagtcacc gttccttgcg caaaggcatg gggtgtcatt acgtaaagta
    19021 cgctggcaat gaaatacgac tgtaatttat tcatggtgcg ggtttaagaa acgtcatttc
    19081 tacgggaata acccctgcgc ctaacatttg ccgactttga cgaacctggc cagtagccga
    19141 atcgacccag aatgtgtttt gccactgttt ttctggcagc gtggaagtca cctcttcctg
    19201 ccagacatta cacagcacat cgcgacccgc aatatgcagc gtctctaatc cagcaaagga
    19261 aaaagtggaa ctcagggtcg cagagcgaaa atggttgtct tcactccaga gaatatcgcg
    19321 cgtccagcgg ctgccgtctt ttattgccag cgcgttacgc agagggtcct gcccggaatt
    19381 tgtcacttcc agcagattat tattaagttt gacggtcttt aaaagacgtc cattgtgggt
    19441 aaccagcatg gcgttatcct gggataacca tttgctttgt tcttgttcta tatatcccag
    19501 taccacaaag attcgttgcc cttcattaag gcgtaaatac atcgtggaat aaggcaacgc
    19561 ctggatctgc tgatccgcta cggtgatatc ctgattatcg aaaaggctgg cgcgaaatgt
    19621 atcgaccata ctttgctggc tgtgcgtaca tcccgcaaga aatagtgcga aaatcgataa
    19681 aataagaggg cgcaccttta atccttgaaa ggttaattaa aaaaatgcaa atctctaaag
    19741 cccggtacga atatcgcaac cgggtattat ttaactttaa tttcgacgcg tttaagcaga
    19801 attagcgcgt agtggacgtg gtggttgagg tgttatgaga accgccatcg ccactgtcat
    19861 ttgctgcaac tactgccaac gtcgccacac caacagccgt tgccacaccg acaactgtcg
    19921 ccgtgccgac agcgccagca gccacgccac cagtggtagc agccgtcgtg gttgcaccgg
    19981 tagtggtcgt tgtggtgcca gtagtacccg tcgttgcctc ggtcccgttt gctgcttcag
    20041 gggcagcaaa cgcagctggc gtcgtcaaca tgaaggccat aaggattgca gaaagcttgt
    20101 gtttcataac ttttccttta ttcatcgcat ggacaatacg tacgcttgag atcaggattg
    20161 tctactttga gtataacatc cttgtgaaat ctccctttcc tcataccact tgcactatat
    20221 acagcgggtg agaaaggcgc gcagagggaa atataagatt gttaactatt cccctctgga
    20281 aatacgactt gtgagcataa gataaatctt agcaaaatac aaattaaaga taagaaaatt
    20341 gttaagaaag ctattaacta ttattg
//
LOCUS       DAZGBC010000087        20038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGBC010000087 DAZGBC010000000
VERSION     DAZGBC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.087"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..640
                     /gene="yfgH"
                     /locus_tag="ACHK1J_003010"
     CDS             122..640
                     /gene="yfgH"
                     /locus_tag="ACHK1J_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7912251.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            656..1195
                     /gene="yfgI"
                     /locus_tag="ACHK1J_003011"
     CDS             656..1195
                     /gene="yfgI"
                     /locus_tag="ACHK1J_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO7912252.1"
                     /translation="MKKVFLCAILVSLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1288..2865)
                     /gene="guaA"
                     /locus_tag="ACHK1J_003012"
     CDS             complement(1288..2865)
                     /gene="guaA"
                     /locus_tag="ACHK1J_003012"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO7912253.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFITVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2934..4400)
                     /gene="guaB"
                     /locus_tag="ACHK1J_003013"
     CDS             complement(2934..4400)
                     /gene="guaB"
                     /locus_tag="ACHK1J_003013"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO7912254.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4562..5932
                     /gene="xseA"
                     /locus_tag="ACHK1J_003014"
     CDS             4562..5932
                     /gene="xseA"
                     /locus_tag="ACHK1J_003014"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO7912255.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5929..6144)
                     /gene="yfgJ"
                     /locus_tag="ACHK1J_003015"
     CDS             complement(5929..6144)
                     /gene="yfgJ"
                     /locus_tag="ACHK1J_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO7912256.1"
                     /translation="MELHCPQCQHVLDQDNGHAHCPSCGEVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6213..7685)
                     /gene="der"
                     /locus_tag="ACHK1J_003016"
     CDS             complement(6213..7685)
                     /gene="der"
                     /locus_tag="ACHK1J_003016"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO7912257.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7803..8981)
                     /gene="bamB"
                     /locus_tag="ACHK1J_003017"
     CDS             complement(7803..8981)
                     /gene="bamB"
                     /locus_tag="ACHK1J_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO7912258.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8992..9612)
                     /gene="yfgM"
                     /locus_tag="ACHK1J_003018"
     CDS             complement(8992..9612)
                     /gene="yfgM"
                     /locus_tag="ACHK1J_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO7912259.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9630..10904)
                     /gene="hisS"
                     /locus_tag="ACHK1J_003019"
     CDS             complement(9630..10904)
                     /gene="hisS"
                     /locus_tag="ACHK1J_003019"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO7912260.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11015..12133)
                     /gene="ispG"
                     /locus_tag="ACHK1J_003020"
                     /gene_synonym="gcpE"
     CDS             complement(11015..12133)
                     /gene="ispG"
                     /locus_tag="ACHK1J_003020"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO7912261.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12160..13173)
                     /gene="rodZ"
                     /locus_tag="ACHK1J_003021"
     CDS             complement(12160..13173)
                     /gene="rodZ"
                     /locus_tag="ACHK1J_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO7912262.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13458..14612)
                     /gene="rlmN"
                     /locus_tag="ACHK1J_003022"
     CDS             complement(13458..14612)
                     /gene="rlmN"
                     /locus_tag="ACHK1J_003022"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO7912263.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14762..15193)
                     /gene="ndk"
                     /locus_tag="ACHK1J_003023"
     CDS             complement(14762..15193)
                     /gene="ndk"
                     /locus_tag="ACHK1J_003023"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO7912264.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15333..16187)
                     /locus_tag="ACHK1J_003024"
     CDS             complement(15333..16187)
                     /locus_tag="ACHK1J_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7912265.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIECAVCQFICPQEAIRGVNSPLRYFRNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNERLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRAP"
     gene            complement(16187..17008)
                     /locus_tag="ACHK1J_003025"
     CDS             complement(16187..17008)
                     /locus_tag="ACHK1J_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFO7912266.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSAPPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(17001..17630)
                     /locus_tag="ACHK1J_003026"
     CDS             complement(17001..17630)
                     /locus_tag="ACHK1J_003026"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO7912267.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17627..20008)
                     /locus_tag="ACHK1J_003027"
     CDS             complement(17627..20008)
                     /locus_tag="ACHK1J_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFO7912268.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYFFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHTCGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGIVEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
BASE COUNT         4967 a         5435 c         4857 g         4779 t
ORIGIN      
        1 taaattattc cccaagtgaa ttttaataaa tttaatctat ccctttatac gcaatacatt
       61 tactttcctc ttttgatgat cttaaatgtc ttatttttcg taatgtgtat aacaaggaat
      121 agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat tcactctggc
      181 aggatgccag tcaaatgctg acgatcatgc cgccgatgtt tatcaaaccg atcaactgaa
      241 taccaaacaa gaaactaaaa ccgttaatat tatttccatt cttcccgcaa aagttgccgt
      301 agacaactcc caaaataaac ggaacgcaca agccttcggc gcgcttattg gcgcagtcgc
      361 tggcggtgtt atcggccaca acgtcgggtc tggcagcaat tccggaacga cggcaggtgc
      421 agttggcggc ggagctgtag gcgcggcagc gggttctatg gtgaatgata aaaccttagt
      481 ggaaggtgtt tctttaacgt ataaggaagg caccaaagtg tatacctcta ctcaggtggg
      541 taaagagtgc cagtttacga caggtttagc cgttgttatt accacgacgt ataacgaaac
      601 gcgtattcag ccaaatacca aatgtcctga aaagagctaa taatcaggag gagtcatgaa
      661 gaaagttttt ctttgcgcca tcttggtctc cttaagctat ccggctatcg cctcatcatt
      721 gcaggatcaa ctctctgctg tcgcagaagc ggaacagcaa ggtaaaaatg aagagcaaag
      781 gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga agcaacgtcg
      841 cgcaaatgcc caggccgccg ctaacaaaag agcggcaacg gcagcggcaa ataagaaagc
      901 tcgtcaggat aaactggacg ccgaagccac tgcggacaaa aaacgcgatc aaagttatga
      961 agatgagcta cgcagcttag agattcagaa acaaaaactg gcgctggcga aagaagaagc
     1021 ccgcgttaag cgcgaaaacg aatttatcga tcaggaactg aagcacaaag ctgcgcaaac
     1081 cgatgtggtg caatctgaag ctgacgccaa cagaaatatg actgaaggcg gtcgcgatct
     1141 gatgaaaagc gtgggcaaag cagaagagaa caaatcggac agctggttta attaatcgat
     1201 gttactaact tcaagcctat gattcttgaa gataaaaaac cctctgtagt aacagagggt
     1261 tttgttcatt catagtgcag ggtcaaatca ttcccactca atggtagctg gcggcttgcc
     1321 gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac
     1381 gcgaccgagg aaatcgtacg gcagatgcgc ccagtgagcg gtcataaagt cgatggtttc
     1441 gaccgcacgc agagaaacaa cccagtcata cttacgacca tcgcccatta cgccaacgga
     1501 acgtactggc aggaagacgg tgaacgcctg gctgactttg tcgtataggt ccgctttacg
     1561 cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac
     1621 ttcacccaga acacgaacgc caaggcctgg tcccgggaac gggtgacggt acagcatgtc
     1681 gtacggtaga cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag
     1741 cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt
     1801 gatgacgtgt gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt
     1861 gccctgcgcc agccacttca cgtcctccag tttcagcgct tcttcatcga atacttcaac
     1921 gaaaacgcga ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga
     1981 caggaagcga tcttctgccg gtacgtgaac aatgttcaga ccaaagtgat cgccaaacat
     2041 atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt
     2101 caggttttta ccgatagcgc ggtgcagcag cattgcggtt acggaggaat ccacaccacc
     2161 agagaggccg aggatgactt tatcgtcgcc tacctgctcg cggatgcgag ctacagcatc
     2221 gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg
     2281 ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata
     2341 gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt
     2401 gatgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag
     2461 cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc tgtcgtttac
     2521 gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa
     2581 ctgcattgcc atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa
     2641 gacatactgc ggcgcacgcg gactgttttc ttcagtagta ctttccgggc cgccggaaag
     2701 aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag
     2761 ttcgcagtaa acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc
     2821 gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga
     2881 ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag
     2941 cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga
     3001 atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca
     3061 cagccggtca gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc
     3121 aggcgacctt tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg
     3181 ttatcgctct ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata
     3241 ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca
     3301 gtacccgcca gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg
     3361 atgtcgccgg agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg
     3421 gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga
     3481 gtcgtacaga tagaacctgg gccgataccg actttaaccg cgctgcaacc agcttctgcc
     3541 aaagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat
     3601 ttagcacggg tttcacggat acgttgcagt acaccttctg agtgaccgtg ggaggagtcg
     3661 atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca
     3721 cctgcgccaa ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt
     3781 ttacgttccg ctttctggaa atctttcacg gtgatcatgc cgatcaggtg gaattcgtca
     3841 tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg
     3901 gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc
     3961 tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt
     4021 tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg
     4081 cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacgccaga ttcgtgtttt
     4141 ttcacacggc gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg
     4201 ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct
     4261 gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca
     4321 gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct
     4381 tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc
     4441 ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc
     4501 cccgaagggc gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca
     4561 catgttacct tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg
     4621 tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac
     4681 acaaccggct tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg
     4741 cgcgatgttc cgcaacagca accgccgggt gaccttccgc ccacagcatg ggcaacaagt
     4801 tttagttcgc gccaatatta cactctacga gccgcgcggc gactaccaga taatcgttga
     4861 gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa
     4921 gttgcaggct gaaggattgt tcgatctgca atacaaaaaa ccacttccct cccctgcgca
     4981 ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt
     5041 aaaacgtcgc gatccttctc tgccggtgat catctacccc accgccgttc agggcgatga
     5101 cgcgccgggg caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt
     5161 gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg
     5221 cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac
     5281 ggatgtgacc attgccgatt ttgttgccga tctgcgtgca ccaacaccgt cggctgccgc
     5341 cgaagtggtg agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg
     5401 gctggagatg gcgatggatt attatctcgc caaccgcaca cgtcgcttta cgcaaattca
     5461 tcaccgatta cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga
     5521 acgcctgcaa aaacggatga gctttgcgct ggaaaatcaa cttaagcgtg ccgggcaaca
     5581 gcagcagcga ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc
     5641 gcaaacgcgc attcagcaac tggaatatcg tttagcagaa accctgcgcg cacagcttag
     5701 cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc
     5761 aacgctggcg cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt
     5821 gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag
     5881 tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa aaggttcatt aagccagcac
     5941 gaattcaacg cgttttttcg aaatcaagcc atgcccgtgc tgacagaagt aatctaccgc
     6001 accgcaggct tttaacacct gcaacggttg atggcagtcc gggcaaagag ctttcatttc
     6061 aataacttcc ccgcaggatg ggcaatgcgc atggccgtta tcctgatcaa gcacatgctg
     6121 acattgtgga caatgcagtt ccatatgaat tcctccggta gccacccctc tacattcata
     6181 gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc
     6241 agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg
     6301 ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg
     6361 aagtagttca tcaagtaacg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga
     6421 atcaccacaa tcggcggatt ataaccaccg gcgtgtgcat atttcagctt cacacgacga
     6481 ccgcgtacca gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata
     6541 gaggtcccca cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta
     6601 ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc
     6661 agacggaagt ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac
     6721 ttattcacca caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga
     6781 tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggcgtcttca
     6841 atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt
     6901 ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg
     6961 tagatactgt cacgcgtcgt accaggcatg tcataaacaa caacgcgctc ttcaccaaga
     7021 atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc
     7081 ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca
     7141 aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc
     7201 cacggcagta gcacatgctc cagcagactt aatacgccac gaccgtgaga cgcggcgatc
     7261 gggtagattt cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga
     7321 ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag atgtttggca
     7381 atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag tacgacgtcc
     7441 gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg
     7501 ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg
     7561 tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt
     7621 agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt
     7681 accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt
     7741 tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac
     7801 gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca
     7861 gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga
     7921 ccatcttcga cgttaatcca gtgcaaataa ccttcactgt caccgaccac caggttgcca
     7981 ttatacagca ccggagaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta
     8041 acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga
     8101 ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca
     8161 ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac aacgccgtta
     8221 acaacgacgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc
     8281 tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga
     8341 ccattatcgc cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc
     8401 aaagagagcg aaggcatatc gaggttaact gtccatttga cagcgccgtc agcttcgttc
     8461 agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct gaccaccggg
     8521 cgcgaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc
     8581 agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca
     8641 ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca
     8701 gaccagattt ctttgccatc atccgcattc agcgctttta ctaaaccagc gcggtccgct
     8761 gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg
     8821 ctaccaacgg aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat
     8881 ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta
     8941 acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga
     9001 caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac
     9061 gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca atgcttcacc
     9121 acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag
     9181 cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc
     9241 tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg cagctttctc
     9301 tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt
     9361 atttttattt tcagcagcaa atttttctgc cgccgggatg ctatccggtt tgccttcgct
     9421 cactgcggta accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg
     9481 gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag
     9541 tgctttgcca ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc
     9601 gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct
     9661 acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg
     9721 ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca
     9781 cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt
     9841 aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa
     9901 gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact
     9961 aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc acggccgccc
    10021 agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgaga
    10081 ctgttagtca cccactcgaa aacggtacgg ttgtagtaat ccagaccacg caccagacgc
    10141 tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc
    10201 tcacgagatt cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc
    10261 acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag
    10321 tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg
    10381 gcgcgtgctt ccagcgaacc gatagaattc agctcaagag ttacgtgttc ggaaataccc
    10441 agcgcgcgcc accagcgggc ggtgagcata atcagttcag cgtcgatatc cggaccttgc
    10501 agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc
    10561 tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca
    10621 tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg
    10681 ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc
    10741 gcacgtttga atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag
    10801 ctgccgagca cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc
    10861 aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg
    10921 aatataaaaa tgaaccctca acgcttccct caatgtttcg ggagccatgc gggttcaatc
    10981 atacacggga agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa
    11041 ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat gcgtgcctcc agctggtcga
    11101 tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagaga ccgcttttct
    11161 tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acccgggcca ttcaccacgc
    11221 agccaataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt
    11281 taactgtacc gataacatca aattcctgac gcgaacaggt cggacaggcg atgaagttga
    11341 tacctcgcga acggatacga agcgatttca aaatatcgaa accgactttg atctcttcga
    11401 ccggatcggc cgccagtgat acgcgcagcg tgtcgccgat gccttcagac agcagcagac
    11461 ctaaaccaat ggcggatttt actgccccgc tgcgcgcgcc acccgcttcg gtgatcccca
    11521 gatgcagcgg ctgatcgatt tgttttgcca gcaaacgata agactcaaca gcgaggaaga
    11581 cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa
    11641 catggcgcat ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca
    11701 gatctttttc cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg
    11761 cacagtcaac caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca
    11821 gacaatcgac gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag
    11881 ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg
    11941 tcggtacgga gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga
    12001 ccgttgcttc gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac
    12061 catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag
    12121 cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc
    12181 ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg
    12241 tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt tgtatggtgc
    12301 ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca attttttacc
    12361 ggtagcatca gtgacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg
    12421 atcagccacc ggcgtggtca cgccagcctg atcggttggc aacggcgcag caccatctgg
    12481 cgttgttgct gccgttggtg ccggggtcgc ggcggtatca acatttgcct gcgaaggcga
    12541 aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg
    12601 cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt
    12661 agttgtcgac gtatttaacg gaacactctg cccctgctcg ctattactgc tcagttccgc
    12721 cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca
    12781 ccaggcaccg ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca
    12841 gccgtcgcgt tttttgcggc gtttaccgag ggaaaaactc tgcatcggcg caacttttgc
    12901 agcccgtagt ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac
    12961 cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc
    13021 cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc
    13081 ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc
    13141 ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat
    13201 tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt
    13261 tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa
    13321 cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga
    13381 ccagacaatt gcaacttaat acttcattca taaaacatta cgccacggta cataaagtaa
    13441 ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta
    13501 cgcagtgtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg
    13561 atatcatcac cacgcgtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt
    13621 gagaaacggt cgatacggct gttcgagctg cgtccatacg gcgcacccgg gaacgggttc
    13681 cacgggatca ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg
    13741 tgttcagtgc cgtcgttaac gtggtcaagc atcacgtatt caatagtgac tcggccctga
    13801 ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac
    13861 tttttgttga tcggcacaat ttcatcacga atttcgtcgt tcggcgcgtg taaggaaatt
    13921 gccagtgcaa cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagtg
    13981 gaaagcgtga cgcgacgttt agacagacca aaaccgaaat catcaagcat gatttccatc
    14041 gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta
    14101 gtgatcgggc gctgaccggt gacttttgct gcgccgacga ttttcgccgc acgccacacc
    14161 tggccgataa tttccgacac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag
    14221 aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct
    14281 tcagggatat acaccgtttc gacgcgctga tcgcctacgg cgatcgccca tttaatggtg
    14341 ccgtcagatg aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc
    14401 agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaaattgtc gcagcaatag
    14461 tgatacatcc acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa
    14521 aactcccgca tctgctgacg attgagatcc agcaggttga tttttccatc tttggtggtg
    14581 acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt
    14641 tatggcccct ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc
    14701 gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt
    14761 attaacgggt gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt tcgcgagcgg
    14821 cagattcgac ggagtcagaa ccgtgggtac cattttcggt caggctgtca gcgtaatcag
    14881 cgcgcagagt accggccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac
    14941 gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt
    15001 caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgagcctgtt
    15061 caacggtcag gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg
    15121 caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgataatg gaaaaagtac
    15181 gttcaatagc catttttacc tctgtaaagt attcgttgtt gtctgtacct gcgtacgaga
    15241 tggcgcggat tataaagagc aacagggccg ttgactattg atgaaggtaa cattttttta
    15301 aaataaaatg agttttagca acaactcgac ttttatggtg ctctcatccc atagcgatga
    15361 cgttggcaat aaagacaatt ttgcgcattt tgctgaaaag taagaaagga tttttgacag
    15421 gttttacaga ctttgcgatt agcatgaaga ttcacaattt ttgccggtag aatcgtcaat
    15481 tcaacggtca ttgcctgatg aaaacaaaca gcaatacaat tttggcatcc gttacacaag
    15541 gcatcattta tttgtaaatg gccttcgtta agcgaaaaga cctgttgctc gcaggcccgc
    15601 cagcatgcac cgcaaagctg acacttttga ggatccatca tcgggcgagc atcactaatc
    15661 tggggccata aataatgtaa ccgacgtaat cctggttgta tttgtgtcat ctgaacatcc
    15721 gcacgctgca catggaacaa acgacgccgg gaaacattca cctcactggt atgtggagat
    15781 accacagacc agccttcctc gccatattgc cgtaagcgtt cgtttaacag caataatgcg
    15841 cgctcccatg ccttcgcctg actcagatca atcgcgatcg ctgtgatccc ctgctcttta
    15901 tgccatagca aaagttcttc cagcgtcggc gcaatagtgg tgaaaggtgc gaccagggtg
    15961 tgattgcgaa aataacgtaa aggggaatta acgccacgaa tagcctcttg tggacaaata
    16021 aactggcaaa ccgcacactc aatacattgt tccgcgtcga ccgctatgcc ctcttctgtc
    16081 agcgttagcg cctgtgccgg gcaagcctcg acacaggcat gacatgttgc gaaacgaaag
    16141 cgttggcgcg cgcattccgc gccaatcgtt atgttatgac ttaacattag ccgatactaa
    16201 aaaagacaaa acgcagcata acttcactcc ccactagcat tacgctaccg aataacgccg
    16261 ccgggatatt ctttttacaa ccaccgacag caaagaacat catcccaatg acggaaattc
    16321 cccagcttag tgtcagtatg ggttttaact gtcccagcat cgctaatggc gcatgtggca
    16381 atgtggctat ctgcatcgca tttgccgtta aatcccctat ccacaacggc tgaaccaaca
    16441 aacgcagcag aaccaggaga gcgatcacca acacgcatcc attacgcagg gcatcaatct
    16501 gcggcaaaat tccgctaata cgaaacactg ctaccagcgc tgaaccgaga attcccactg
    16561 tacctaagaa cataatcagc gtgttgtaat gctgccaggt gataatcgac gtatggatgt
    16621 aaacttgcgc catgcagaaa acatcaacaa tcccgaccac tgccgctagc ataagcagga
    16681 gttgccagcc gcctttgcgt aacatgacca gtaatgtgga taaacccaat agcgccagat
    16741 acagcgcacc aaagataatt tcccgactca gccatgaact ggaaaagtga cttagcgcat
    16801 gcatcgcatt aagtggataa cccaggtgta aggtagaaat cagtaaccct acggcccctg
    16861 ccaaaaaagc caacagtaat ggcggccgca taaccggggg ggcgctggag gtgattaaaa
    16921 tatgacgatg aagaaacgcc agccacaacg tcataccgac cgatgcctgt aacaacagcg
    16981 taaaagccat taacggtaat tcatgcatga tgtgacgcct ccttttccgc gccctgatgc
    17041 ggtttaatca ccagatttgg ctgcgtaatc gacgaatcag gtaaaccttt cacatcactg
    17101 acatcgccat acttttcacg caactcatca atagggccga acttaatcgc ccccaacggg
    17161 cacgttgcca cgcaaatggg ttgttctcct ttagcttgca gatcaataca gaaatcgcat
    17221 ttcgacatct gcccggtctg ctcattcatt tgtggtgcgc cgtaaggaca ggaccaagcg
    17281 cagtaaccgc aaccgacgca cttatcagta ttcacgcgca caatgccgtc tccgggacgc
    17341 ttatgcatcg ccatcgtcgg gcaatttttg gtacagatcg gatccgcgca atggttacag
    17401 gaaacagaaa gggtataagc aaagacatta tgactcacac cgccctctcc agtggcgatg
    17461 aagccacctc cgttcacttc atacacgcgg cgaaaacgtc gcccaacctc aaggttgttt
    17521 ttgtctttac aggccacctg acacgcttta cagcccgaac aacgcgaaga gtcaataaag
    17581 aaccctaatt gtttgtcgct gacagatgca aattctttaa attggctcat gctttctcaa
    17641 cctccaccag cattgtttga tgcgaattcc ctttcgccag cgccgtaatg cgggtcgaac
    17701 tcagtacatt ggcacagcct ccgcgatcga taccttgtgc atccggttgc caccatgccc
    17761 ccgcctgcat agcaactacg ccagggatga tacggggggt tatttccacc gggataatgc
    17821 tcacaccacg atcgttataa atttttacgc tatctccttc gacaatcccg cgctgttttg
    17881 catcttgcgg attcagccag agtttttgcg tctgaacttc ttgcagccac gggttagcat
    17941 actgggtgga attagcacgg tttttccctt tccaggtgat gagctgcagc gggtatttcg
    18001 ccgtcagttt atcttctggc ccttcaaagg cagggacata gtgcgaaagc gcgggaattt
    18061 ctggatcttg catatcaaaa agacgcttag agaagatctc aattttgccg gaaggcgtcg
    18121 ggaaaggatt attctgcgga tcgcgaatat ttgcttcgaa agcgatgtgc ggcgcgcttt
    18181 tgaaaagatg tcgacgtgtt ttttgcaggg tcgcgaagtc cggcaaattt tcatccggca
    18241 tcgccagacg agtttgctcc cagatatgct cgatccattg cttctcatca cgtccttgac
    18301 tgaactcggc ttcgacaccc agttttttcg ccacgtcacg caaccagtca taatcggtgc
    18361 gacgttcaaa atccggctca atcagttttt ctgacagaat aagatagctc gcagttcccc
    18421 atgtttcgcc gatattccag cgttccataa agctggtttc cggcagcaac acatcggcat
    18481 atttcgcgct cggagtcatg aacagatcgc tgagtacgat gaattctatt ttagattcgt
    18541 cttcaaggag cttcgcggcc tggtggacgt ccgggttctg gttcgccaga taattacctg
    18601 ccagtgagaa cagcaggcgg atattactgt cgagtttatc gacaccttta agcccatctt
    18661 gcggcgtaac tttagaagca tcatccgccg cctgcatcca gttcataatc gagattttcg
    18721 cttgtaccgg attttctggc atatctggtc caacgcagaa tttacgatta gaactaccgc
    18781 cgtaacctgc cgcccagcca cctttgatgc cgacgttacc ggtaatcgag gccagtaaag
    18841 ttgaacctcg ggcggtgcgt tcgccgcaag tatgtcgctg tggcccccat ccctgaatca
    18901 acgctgcggg ttttgtagtg gcgtaatcgc gtgccagttg acgaatggat tgtgctggaa
    18961 cgtgggtaat tttttctgcc cattccggcg ttttgtgtat cccatctttt gcgccaaaaa
    19021 ggtaggcaac caacgattcg ttggctggta cgccttctgg catagagttt tcatcaaaac
    19081 cgagagtata tgtgtcgata aatgtcttat cgtggagatt ctcgctgata atgacgtaca
    19141 tcatcgcgtc catcaacgca ttgtccgtgg tcggcagcag cgggatccac tgatcggcaa
    19201 gcgaagatac agtgtcggaa taacgcggat ctaccacgat aaaacgcgta ccgttttgct
    19261 tcattttttg aaaaaagtaa ttggtgtgac cgaagatggt ttccgttggg ttatgccccc
    19321 agaggatcac cagtttggta tccagaagag tatccatcga gttaccgctg gccgccacgc
    19381 catacgtgta aggcgttgcc gccgcagtat tacccagact aactgagtga taatattcaa
    19441 gataaccacc ggttaaattt aacagccgac gcgccatcgc atcgccagaa aatgttccac
    19501 cagaaacagc cgtcccgaca tggacataac gtgaagccgg gccatattgt tgcgtgatcc
    19561 ttttcaggtt atcggcaatt aaggtcgtcg cctcatccca tgaaatacgc tcaaatttac
    19621 cctcgcctcg tttacctacc cgcttcatcg gatatttcaa acggtctgga tgataaacaa
    19681 atttacgata gccgcgacca cgaacacagg cgcgcataat cggcatttgt ggatccagtt
    19741 cgttatcggg aagcgtggta atttgcgtca ccacaccgtc gcgcatatgc gcccgaatat
    19801 cgcacttacc accgcagtca aatgtgctac aggtgggcac gactttatca gccaccggag
    19861 ctgtaacttc tggcgacgca tctggggatt gcgcctttgc ggtagaagaa ataaaaggca
    19921 aagtgataag cgcggaagtc gcttgtaaaa aactacgtcg tgatacttgc ggaaatgtaa
    19981 tgcgttcctt ttcgtctggc gtatccatct tgcgttcccg aaaatgaaaa aataaata
//
LOCUS       DAZGBC010000088        19840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGBC010000088 DAZGBC010000000
VERSION     DAZGBC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.088"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(22..210)
                     /gene="bcsR"
                     /locus_tag="ACHK1J_003028"
     CDS             complement(22..210)
                     /gene="bcsR"
                     /locus_tag="ACHK1J_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO7912269.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            483..2054
                     /gene="bcsE"
                     /locus_tag="ACHK1J_003029"
     CDS             483..2054
                     /gene="bcsE"
                     /locus_tag="ACHK1J_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO7912270.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2051..2242
                     /gene="bcsF"
                     /locus_tag="ACHK1J_003030"
     CDS             2051..2242
                     /gene="bcsF"
                     /locus_tag="ACHK1J_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO7912271.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2239..3918
                     /gene="bcsG"
                     /locus_tag="ACHK1J_003031"
     CDS             2239..3918
                     /gene="bcsG"
                     /locus_tag="ACHK1J_003031"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO7912272.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(4005..4112)
                     /locus_tag="ACHK1J_003032"
     CDS             complement(4005..4112)
                     /locus_tag="ACHK1J_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7912273.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4488..4595)
                     /locus_tag="ACHK1J_003033"
     CDS             complement(4488..4595)
                     /locus_tag="ACHK1J_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7912274.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4971..5078)
                     /locus_tag="ACHK1J_003034"
     CDS             complement(4971..5078)
                     /locus_tag="ACHK1J_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7912275.1"
                     /translation="MTLAELGMAFWHDLVAPVIAGILASMIVNWLNKRK"
     gene            5554..6825
                     /gene="yhjV"
                     /locus_tag="ACHK1J_003035"
     CDS             5554..6825
                     /gene="yhjV"
                     /locus_tag="ACHK1J_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO7912276.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(6855..7859)
                     /gene="dppF"
                     /locus_tag="ACHK1J_003036"
     CDS             complement(6855..7859)
                     /gene="dppF"
                     /locus_tag="ACHK1J_003036"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO7912277.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(7856..8839)
                     /gene="dppD"
                     /locus_tag="ACHK1J_003037"
     CDS             complement(7856..8839)
                     /gene="dppD"
                     /locus_tag="ACHK1J_003037"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO7912278.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(8850..9752)
                     /gene="dppC"
                     /locus_tag="ACHK1J_003038"
     CDS             complement(8850..9752)
                     /gene="dppC"
                     /locus_tag="ACHK1J_003038"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO7912279.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(9762..10781)
                     /gene="dppB"
                     /locus_tag="ACHK1J_003039"
     CDS             complement(9762..10781)
                     /gene="dppB"
                     /locus_tag="ACHK1J_003039"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO7912280.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(11089..12696)
                     /gene="dppA"
                     /locus_tag="ACHK1J_003040"
     CDS             complement(11089..12696)
                     /gene="dppA"
                     /locus_tag="ACHK1J_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO7912281.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(13441..13517)
                     /locus_tag="ACHK1J_003041"
     tRNA            complement(13441..13517)
                     /locus_tag="ACHK1J_003041"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13481..13483),aa:Pro,seq:cgg)
     gene            complement(13609..15300)
                     /gene="eptB"
                     /locus_tag="ACHK1J_003042"
     CDS             complement(13609..15300)
                     /gene="eptB"
                     /locus_tag="ACHK1J_003042"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO7912282.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAVAN"
     gene            complement(15555..16085)
                     /gene="lpfE"
                     /locus_tag="ACHK1J_003043"
     CDS             complement(15555..16085)
                     /gene="lpfE"
                     /locus_tag="ACHK1J_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO7912283.1"
                     /translation="MKFNLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            complement(16090..17145)
                     /locus_tag="ACHK1J_003044"
     CDS             complement(16090..17145)
                     /locus_tag="ACHK1J_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7912284.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRSV
                     DSMKYYTLTEELEVATKIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTITQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDASSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            complement(17160..19733)
                     /gene="lpfC"
                     /locus_tag="ACHK1J_003045"
     CDS             complement(17160..19733)
                     /gene="lpfC"
                     /locus_tag="ACHK1J_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO7912285.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASTFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPALNLVPAEACVPFDEIIPQASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSSGSSSSYVDDNGTVHHDDGNDTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIIPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDEEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSASPRFGQLDLIYGLPWGMTAYGGVLISNNYNAFALGIGKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWNKSPEWDESDRLWSFNISVPLGRAWSNYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAIVPYAMPYRENEISLRSDSL
                     GDDVDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQ
                     NEVSSIVGEEGQLYISGMPEEGRVLIKWGNDASQQCVAPYKLSLELKQGGIIPVSANC
                     Q"
     gene            complement(19762..>19840)
                     /locus_tag="ACHK1J_003046"
     CDS             complement(19762..>19840)
                     /locus_tag="ACHK1J_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="binds with fimbrial subunit LpfA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB"
                     /protein_id="HFO7912286.1"
                     /translation="SVSKIKYNAINDFGSSAEMMTKNVD"
BASE COUNT         4721 a         5289 c         4870 g         4960 t
ORIGIN      
        1 gtacggccat tcaccaatcc cctacttttg ttgcgcaaac tctgccagca acggccagcg
       61 ttttaatgcc gcggccaact gttcgcgttg ggaaatatcg gcataatcaa tatcaggcag
      121 tgaaaatgcc tgctttaacg ccaaaatatc attctggaag atatagccta tcgcgggatc
      181 aggcagagta tctggttcat tgttattcat tttgcttgat ccctatgaaa taaccgtacc
      241 gttcatgaga caattctaac cgtgagcatt tcgccggttt attgtttacg gcgagacggg
      301 aatttgctca ctttttgact ttccgttacg caacagaaaa agttaaaaac gaaaagacta
      361 aaatcacacg tcttgtgacc tgctaaattg attcacatgc taaatctgat aagttttaat
      421 ttcaatggta ggtttatttc ttagctttcg ctagtaaact gataatcaga caaaatggtg
      481 acatgaggga cattgtggac cctgtattct ctatcggtat ctcatcatta tgggatgagc
      541 tgcgacatat gccagcaggc ggcgtctggt ggtttaacgt cgatcgccat gaagatgcta
      601 tcagtctggc gaatcaaaca attgcatccc aggctgaaac cgcacacgtc gcggtcatta
      661 gcatggacag cgatccggcg aaaatctttc aattagatga ttctcaaggg ccggaaaaaa
      721 taaaattatt ttcaatgcta aatcatgaaa aaggtctata ctatttggcc cgtgatttgc
      781 agtgttctat tgatccccat aattaccttt ttattcttgt ttgcgcaaat aacgcatggc
      841 aaaacattcc tgccgagcgg cttcgctcat ggttggataa aatgaataaa tggagcaggt
      901 taaaccattg ttcgcttttg gtaattaatc ccggaaataa taacgataaa caattttcat
      961 tgttgcttga ggaataccgt tcactttttg gtcttgccag tttgcgtttt cagggcgacc
     1021 aacatttgct ggatattgcc ttctggtgca acgaaaaagg ggtcagcgcc cgtcagcagc
     1081 ttagcgttca gcaacaaaat ggtatctgga cattagttca aagcgaagag gcggagatcc
     1141 aaccacgcag cgacgaaaaa cgcattctga gtaatgttgc tgtactggaa ggtgcgccgc
     1201 cgctatcgga acactggcaa ctgttcaaca ataacgaagt cctgttcaat gaagcccgta
     1261 ccgctcaggc ggcgacggtg gtcttttctt tacaacaaaa tgcgcaaatc gagccactgg
     1321 cccgcagcat tcatactctg cgtcgccagc gcggtagtgc gatgaaaatc ctcgtacggg
     1381 aaaataccgc tagcctgcgc gccaccgatg aacgtttgtt attggcctgc ggtgcaaata
     1441 tggttatccc gtggaatgcg ccactctccc gctgtctgac gatgatcgaa agcgtgcaag
     1501 ggcagaagtt tagtcgctat gtgccggaag atatcactac cttgctgtca atgacccagc
     1561 cgctcaaact gcgtggtttc cagaagtggg atgtgttctg taatgccgtc aacaacatga
     1621 tgaataaccc tcttttgcct gcccacggta aaggtgttct ggttgcccta cgtccggtac
     1681 cgggtatccg cgttgagcaa gccctgacgc tatgtcgccc taaccgtacc ggcgatatca
     1741 tgaccattgg cggtaatcgg ctggtgctgt ttctctcatt ctgtcggatt aacgatctgg
     1801 ataccgcgtt gaatcatatt ttcccattgc ctactggcga cattttctca aaccgtatgg
     1861 tctggtttga agatgatcaa atcagtgccg agctggtgca gatgcgcctg cttgccccag
     1921 aacaatgggg catgccgctg cctttaacac aaagttctaa accggtcatc aatgccgagc
     1981 acgatggtcg ccactggcga cgaataccag aacccatgcg actgttagat gatgctgtgg
     2041 agcgctcatc atgatgacca tcagcgatat cattgaaatt attgtcgttt gcgcactgat
     2101 atttttcccg ctgggctatc tggcgcggca ctctttgcga cgcattcgcg acaccttacg
     2161 tttgttcttt gctaaacctc gttatgttaa accggctggg acgttacgcc gcacggaaaa
     2221 agccagggca accaaaaaat gactcaattt acgcaaaata ccgccatgcc ttcttccctc
     2281 tggcaatact ggcgcggcct ttccggctgg aacttctatt ttctggttaa gttcggcctg
     2341 ttgtgggcgg gatatcttaa cttccatccg ctcctcaatt tggtgtttgc cgcgtttctg
     2401 ctgatgccca ttccgcgcta cagcctgcat cgcttgcgcc actggattgc cctgccgatc
     2461 ggctttgctt tgttctggca tgacacctgg ttgcctggcc cggaaagcat aatgagccag
     2521 ggttcgcagg tggcggggtt cagtaccgat tatttaatcg accttgtcac acgctttatt
     2581 aactggcaga tgattggggc catttttgtt ttattagtgg cctggttatt cctgtcacaa
     2641 tggattcgca ttaccgtttt tgtggttgcc attctgctgt ggctgaacgt acttaccctg
     2701 gcgggaccaa gtttctcctt gtggccagcc ggtcaaccga cgaccactgt aacaacgacg
     2761 ggtggtaacg cagcggcaac cgttgcggcg acgggtggcg caccggtagt gggtgatatg
     2821 cccgcacaaa ctgcaccgcc aacaacggcg aaccttaacg cctggctgaa taatttctat
     2881 aacgcggagg cgaaacgtaa atcgatcttc ccgtcttcgc tgcccgctga tgctcagcca
     2941 tttgaactac tggtgattaa tatttgttcg ctttcctggt cggatattga agccgccggg
     3001 ttgatgtcgc atccgctgtg gtcgcatttc gatattgagt tcaagaactt taactccgcc
     3061 acctcctaca gtggcccggc ggcgatccgt ttactgcgcg ccagctgcgg gcagacttcg
     3121 cacactaatc tgtatcaacc ggcaaataac gactgctatc tgtttgataa cctgtcgaaa
     3181 ctgggcttta cccagcacct gatgatggga cataacggcc agttcggcgg ttttttgaaa
     3241 gaagttcgcg aaaatggcgg catgcagagc gaattgatgg atcaaacaaa tctgccggtt
     3301 attttgctgg gctttgatgg ttcgccggtt tatgacgata ccgccgtgct taaccgctgg
     3361 ctggacgtta ccgaaaaaga taaaaacagc cgtagtgcca cgttctacaa cacgcttcca
     3421 ctgcatgacg gcaaccatta tccgggcgtc agcaaaacag cggattacaa agcgcgggcg
     3481 cagaaattct ttgatgaact ggacgccttc tttactgaac tggagaaatc gggtcgtaaa
     3541 gtgatggtgg tcgtggtgcc ggaacacggt ggcgcgctga agggcgacag aatgcaggta
     3601 tctggcctac gtgatatccc tagcccgtct atcaccgacg tccccgttgg ggtgaaattc
     3661 ttcggcatga aggcaccaca tcagggggca ccgattgtca tcgaccaacc gagcagcttc
     3721 ctggctatct ccgatctggt ggttcgcgtt cttgatggca agattttcac cgaagacaat
     3781 gttgactgga aaaaactcac cagtgggttg ccacaaacag caccggtctc cgagaactca
     3841 aatgcagtag ttattcaata ccaggataaa ccgtacgttc gcctgaacgg cggcgactgg
     3901 gtgccttacc cgcagtaagc acgaaaaagg ccgcagagtt tcacccctgc ggcctggtcc
     3961 gggcgcaaat tgccattacg gtagcctgac gcccgtctga cacgttactt ccgcttgttc
     4021 agccagttca cgatcatact ggcaagaatg ccagcaatga ccggagccgc taaatcatgc
     4081 cagaaggcca tgcccagctc tgcgagcgtc atatagccgc ctgtgttgta atgacaacgt
     4141 ttcgcggcta ttcttgaatg gtctagatgc aagaatggcc cccgtgatgt tgtcaggtgc
     4201 atacctgcaa cgtgcggggg ttttctctct ccagcaacca atgccaccag ggattaagcc
     4261 cccgcaacat tgcgcctcac cggaatcctt ccggcttgac gctgattcta cgactgtttt
     4321 tctgttccgg aatccgtcac gtcttcactc tgtgctgcgc attctgcaga atattatccg
     4381 cttgcaatcg ttgcattttt cttacgaaaa aggccgcaga gtttcacccc tgcggcctgg
     4441 tccgggcgca aattgccatt acggcagcct gacgcccgca tgacacgtta cttccgcttg
     4501 ttcagccagt tcacgatcat actggcaaga atgccagcaa tgaccggagc cgctaaatcg
     4561 tgccagaagg ccatgcccag ttctgcgagc gtcatatagc cgcctgtgtt gtaatgacaa
     4621 cgtttcgcgg ctattcttga gttgttcagg ttcaagaatg gcccccgtga tgttgtcagg
     4681 tgcatacctg caacgtgcgg gggttttctc tctccagcaa ccaatgccac cagggattaa
     4741 gcccccgcaa cattgcgcct caccggaatc cttccggctt gacgctgatt ctacgactgt
     4801 ttttctgttc cggaatccgt cacgtcttca ctctgtgctg cgcattccgc agaatattat
     4861 ccgcttgcaa ccgttgcatt tttcttacga aaaaggccgc agagtttcac ccctgcggcc
     4921 tggtccgggc gcaaattgcc attacggcag cctgacgccc gcatgacaca ttacttccgc
     4981 ttgttcagcc agttcacgat catactggca agaataccag caatgaccgg agccactaaa
     5041 tcatgccaga aggccatgcc cagctctgcg agcgtcatat agccgcctgt gttgtaatga
     5101 caacgtttcg cggctattct tgagtggtct aggttcaaga ttagcccccg ttatgttgtc
     5161 aggtgcatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat
     5221 aaagcccccg caacattgcg cctcaccgga acccttccgg cttgccgctg attttacgat
     5281 tgctttttac tgtcagcacc cggcaatctt cctcattgcg cgatgccttc cagcttaatt
     5341 tctttatttg caatagttgc agttcatatc cgggctgact ccagactggt agtgaaatgc
     5401 gcaattttgt agaattgcgc ctcctcgcga cctccccagt gaatctgttg cctcattttt
     5461 tacatactgc gtattcgact tctccacctg ttgcgcaaga gaaactgggt ttattcattt
     5521 ttgcgaggcc gacttctttc tggacaggac tttatgcagc acaacacact accgaaacac
     5581 gatcagaaac tgccgtttac acgctacgac ttcggctggg ttttattatg cataggcatg
     5641 gcgattggtg ccggaaccgt gctgatgcca gtacaaattg gcttgaaggg tatttgggta
     5701 tttattaccg cagcgatcat tgcttatcct gccacctggg tagtgcagga catttattta
     5761 aaaacccttt ctgaaagcga ttcctgtaat gactacaccg atattatcag tcattacctg
     5821 gggaagaact ggggaatttt cctcggggtt atctactttt tgatgattat ccacgggatt
     5881 tttatctact ctctctccgt ggttttcgac agcgcctcgt acctgaaaac cttcggttta
     5941 accgatgccg atctttcaca atctctactt tataaagtcg ctattttcgc cgtactggta
     6001 gcgattgcgt ctggtggtga acgattactg tttaagattt ctgggccaat ggtggtggtc
     6061 aaagtaggga ttattgtcgt gttcggtttt gcgatgatcc cgcactggaa tttcgccaat
     6121 ataaccgcct tcccgcaagc ctccgtcttt tttcgcgatg tcctgcttac cattccattt
     6181 tgcttctttt ctgcagtatt tattcaggta cttaacccaa tgaatattgc ctatcgtaaa
     6241 cgggaagcgg ataaagtact ggcaacccgg ctcgcgctgc gtacccaccg aattagttat
     6301 atcacgctca tcgcggtgat actgtttttt gccttttcgt ttaccttctc aattagccac
     6361 gaagaagccg tttctgcctt tgaacaaaat atctcggcac tggcactggc cgcgcaggtg
     6421 atccctgggc atatcattca tatcacctct acggtgctga atatctttgc cgtactgacc
     6481 gcattctttg gcatttatct cggtttccac gaggccatta aaggcattat tctcaatctg
     6541 ttaagccgaa ttattgatac caagaaaatt aactcacgcg tgctgactct ggcgatctgc
     6601 gcttttatcg tcattacgtt gacgatttgg gtttcgtttc gtgtatcggt gctggtgttc
     6661 tttcagttgg gaagcccgtt atatggcatt gtgtcgtgcc tcattccgtt tttcctgatc
     6721 tataaagtcg cacaactgga aaaacttcgc ggatttaaag cctggctgat tctgctttac
     6781 ggcattttgc tatgcttgtc gccactgttg aagctgattg agtaaaccgg agcgcatggc
     6841 cccggttttg tgagttaacg ctgcggattt tcatcctgat caacagcaaa acaagctacc
     6901 agttgaccgc cgtagtcttt tagctgcggc tgcaactggg tgcaggggcc gaagcgccga
     6961 cgacagcggg cgttaaacgc gcagcccggc ggtggattca gcgggcttgg cagttcaccg
     7021 gtgagtttga tgcgctcgcg gcgatcgtcc ggattcaggc gcggcgtcgc ggaaagtagc
     7081 gcctgagtgt acggatggcg cgggttattg aagatttggt ctttcgttcc cttctccacg
     7141 cagcggccca ggtacatcac catcacttca tcagcaatgt gctccaccac cgacaggtcg
     7201 tgggagatga agacataaga aagccccagt tcctgctgca aatccatcat caggttgagc
     7261 acctgtgcac gcaccgagac gtccagcgcg gagaccggtt catcggcaat caccacgtcc
     7321 gggtcgagca tcagaccacg ggcaatagcg atacgctgac gttgtccgcc ggagaacata
     7381 tgcggatagc ggtcatagtg ctcggttttc aggccgactt tcgccatcat cgacagggct
     7441 ttttcccgac gctgttcttt gcttaagctg gtgttgatca gcagcggctc ttcaagaatt
     7501 tgcccgactt ttttacgcgg attcagcgaa ccgtaagggt tctggaagac aatctggatt
     7561 ttctgccgac gcagcttctg cgcctgcgga tcgtgcttaa gcagatcctg cccctggtaa
     7621 tacagctcgc cgccggtggg cgtttcaatc atcgtcagca accgaccgag ggtcgattta
     7681 ccgcagccag attcacctac taccgccagc gttttgccac gttcaaggtt aaacgaaacg
     7741 ccgtccagcg ccttcaccag acgttccgga gcgaacatac ctttctttac cggataatgt
     7801 tttttcaggt cgatagcctg caacagcggt tgttgcgagg tggcctcttg cgtactcata
     7861 gtgtcggcct cccggcatca tcaagtgggt aatggcattt ggactgacgc ccgtcagcga
     7921 gcatattcag cgccggttct tcagcgcgac atctgtcagt ggcatagggg cagcgcgggt
     7981 taagcaggca gccgttcggg cggtcgtact tgccgggaac aacgccaggc aacgatgcca
     8041 gacgttcttt gtcctgagca aattccggca gcgcacgcag caatgcctga gtatacgggt
     8101 gacgcggcgc atggaagatg gcgtgcgcat cgcccgtttc caccacctga ccggcataca
     8161 tcacgatgat tttatgtgcc gcttccgcca ccagcgccag gtcatgggta attaacacca
     8221 gcgccatgtt ctctttctgc tgtagctcca gcagtagttc gatgatttgc gcctgaatgg
     8281 tcacgtccag cgcagtggtc ggttcatcgg caatcagcag ttttggccga caggcaatgg
     8341 ccatcgcaat catcacgcgc tggctcatgc cgccggaaag ctgatgcggg taaacatcca
     8401 gacgcgatgc cggatcggga ataccgacct gattcagcag gtcaatcgcc cgctgacggc
     8461 gagtgctctt attaccgccc tgatgcacct taatcgcttc cataatctgg aaaccaacgg
     8521 tgtagcacgg gttaaggctg gtcatcgggt cctggaagat catcgccact tcggcaccca
     8581 ccagattgcg gcgctctttt tctgagatac gctgcaaatc ctgaccgtta aactccagct
     8641 tttccgccat cacgcggccc ggataatcaa tcagccccat aatcgccagt gaactgaccg
     8701 acttaccgga gccggactca cccacaatcc cgaccacttc gccctgtttc acgctgtagc
     8761 taatgcggtc tacggcgcgg aacggtgcgc tttcgtcgcc gaaatgcacc gataatttat
     8821 ctacatttaa taacgccatc tcgaacctct tactgcttca gtttgggatc gagcgcgtca
     8881 cgcagaccgt cacccatcag gttaaatgcc agcaccgtca gcaggatcgc cagacccggg
     8941 aaggtcacga cccaccaggc gctttgcgcg aactgcaaca cgtcggagag catggtgccc
     9001 cactccggcg ttggcggctg cgcccccatg ccgaggaaac caagagcagc catatcgaga
     9061 atggcgttag agaaaccgag cgacgcctga acaatcagag gcgcaaggca gttcgggaag
     9121 atgttaataa acatctgacg catcgccccg gcacctgcca cgcgagacgc ggtaacgtaa
     9181 tcgcgattca cctccaccag cacggcggcg cgtgttaagc gcacatagtg cggcaaggca
     9241 acgaaggtca gcgccagcgc ggcgttacca atcgacgggc cgaaaatcgc caccagcacc
     9301 agtgccaaca gcagacttgg cagcgccagc atgatatcga ccacgcgcat gatgatgtta
     9361 tcgaccaggc cgccaaagta accggcgatc aggccgagaa taacgcccat aatcagcgat
     9421 aacaccacca ccagacagcc aaccagcagc gataaacgcg cgccgtacat cagccgcgac
     9481 agcacatcac ggcctacgtc atcggtgccc agcaagtgcg ccatgctgcc gccttcctgc
     9541 caggcgggcg gggcaagcag cgcatcgcgg aattgttccg ccgggttata gggagcaatc
     9601 cagttggcaa agatcgcgat aaacagcacg atgacgacgt aaaccagccc gacgaccgcg
     9661 cctttgttgc gtttaaaata gtgccagaac tcctgtaacg gggtcatcgg caccggtgcg
     9721 ctaatcactt tattttcagt aacctgtgac atgatgtccc cttacttctt atgacgaata
     9781 cgcgggttca ccacgccgta cagcagatcg accagcagat tgacgaggat aatcatcgtc
     9841 gccaccagca atacgccgcc ctgcactacc ggatagtcgc ggcgttgcag tgcgtcaatc
     9901 aaccagcgcc ccagaccggg ccacgagaag atggtttcgg tcagaatcgc ccccgccagc
     9961 aatgttccca cctggaggcc gataacggtc actaccggca gcatcgcgtt acgcagcgca
    10021 tggacgataa tcaccctaag gcgggtcaat cctttggcgc gcgcggtgcg gatgtaatct
    10081 tcacccagca cttccagcat cgaggagcgt gtcatacgta caatgaccgc cagcggaata
    10141 gtacccagca caatggcggg caagatcata tgggcgacgg catcgataaa gttaccgtct
    10201 tcaccccaga tggcggtgtc gatcagcata aaaccggtta acggattgga atcatcgagg
    10261 aacaccatat cgctcacgcg accggagacg ggtgtcaggt tccagtgcac cgaaaccagc
    10321 atgatcagca tcatgcccca ccagaagata ggcattgaat aacctgtcag cgccaggcca
    10381 accgctgtgt gatcgaaaat ggaaccgcgt ttaaccgcag ccagcacacc aaccggaata
    10441 ccgactgccg tagcaaaaat catcgcgcag acgccaagtt ccagcgtggc ctggaagcgc
    10501 ggcacgaact cttcccaaac cgggatgcgg cttttcattg aaatgcctag atcgccatgc
    10561 ataacgcccc aaatgtaatg gagatactgc tgccacatcg gtttatctaa gccgagttca
    10621 gccagcagct gcgcgtgacg ctctggggag atcccacgct cgcccgccat gatcatcacc
    10681 ggatcgcccg ggatcatgtg gacaaaggca aatgtgagaa gggtaatacc gataaacgtg
    10741 gggatgacga gtcccaaacg tcggagaata aactgcaaca taacccggat tctctgtagt
    10801 gacccgcagc ctggaactgc gtgtctgtat tgctcacaaa tcaattcccg cccttgtctg
    10861 acagggaatg aagtattgct gccggatggc gctgcacgca gaggcacagc accaccgggt
    10921 atgcagattg ttgacaaaga gcgctttgtt tatgcgggat gcggcgttaa cgccttatcc
    10981 ggcctacaaa atcttgcaaa ctcaatacat tgcagaattt tcgtaggcct gataagcgga
    11041 gcgcatcagg cagttttgcc tttgccatca gtcttgtatg gcttttaatt attcgataga
    11101 gacgttttcg aagtgatgtt tgcctaatgg atcaaccaca tagcctttaa cttctttgcg
    11161 taccggttca aacacggtgg agtgagcgat gatcagtgcc ggagcctgat cgtgcatcac
    11221 cacctgcgct tgcttgtaca gttcaacgcg tttgttatgg tcgtcggtag cacgcgccgg
    11281 ttgaatcaga tcttcaaacg gtttgtagca ccattttgag tagttggagc cttgttcaga
    11341 ggcggcgcag ctgaacaggg tggcgaagaa gttatccgga tccccgttat cgccagtcca
    11401 gcccatcatt accgtctgat gctcgccatc tttcgcacgc ttgagatact caccccattc
    11461 gtaggtgacg attttggcct gcacgcccac tttcgcccag tctgcctgaa tcatctccgc
    11521 catgcggcga gcgttcgggt tatacggacg ttgtaccggc atcgcccaca ggtcgatgga
    11581 gaaacctttt tccagacccg cttctttcag caaggctttc gctttttcag gatcgtaggt
    11641 gtagtcctga acgtcgtcgt tatagcccca catggttggc gggatcaggt ttttcgctgc
    11701 cacgcccgcg ccctgataaa ccgctttaat gatggcatct ttgttcaccg cgtaggtcag
    11761 agcctggcga actttcacgt catcgagtgg ttttttctgc acgttatacg agagataacc
    11821 gacgttcagc cccggcattt ccatcaggtt gatggattta tcctgcttca tgcgagcgat
    11881 atctgccggg ttcgggtacg gcatcacctg gcattcattc ttctgcaatt tcgcgtaacg
    11941 cacggaagca tcaggggtaa tagagaaaac cagcgtatcg atctgcggtt tggtgcccca
    12001 gtagccatca aacgctttgt agcggatacg ggaatctttt tggtactgct gtaactggaa
    12061 cggaccggtt ccgattgggt tgaggtccag tttttccggt gtaccggctt tcatcatcgc
    12121 gtcagcatat tcttttgaca gaatagaggc gaagtccatt gccaggtcag cgaggaacgg
    12181 cgcttccggg cgagtcagca cgaactgaac tgtgttgtcg tccacctttt tcacttcgct
    12241 gatcagctcc ggcaagccca tgccttcgaa gtattcgtag ctaccgccag aaactttatg
    12301 gtacgggttt tgcgcgtttt tctgacgatc gaacgagaac accacgtcat cggcattcat
    12361 ttcgcgcgtc ggtttgaatt ctttattgtc gtgccacttc acacctttac gcagatggaa
    12421 ggtataggtt ttaccgtctt cgctgacttc ccacttttca gcgaggcccg ggatcacttc
    12481 ggtggtgccg attttaaatt caaccaggcg gttataaagc ggtacggaag aggcgtcata
    12541 ggtggtaccg gaggtaaaca gctgcgggtt aaacccttcc ggagatcctt ctgagcaata
    12601 aaccagagtt ttagcctgaa cacttgctgc gacggtcata gccaccaggc tgagaccaag
    12661 cttcagcatc cctgactttt tcaaggaaat acgcattatt ctgctccaat tgtgatgttt
    12721 gttgttttaa ccctttgcag tgggttgtcg ctgcctgacc ttttttgttt tttgcccggt
    12781 cgggtcaacg ttatgaggtg gggatgccgt actaattaac atcagtgtaa cagtgcggat
    12841 cctccataaa atgcccctcg tgacctacaa tctgtcaaca gaatgtgaaa acgtcaatac
    12901 agccaaccgg gatttacacc aacggtgaga atccacaaac aaagattaaa aaaacttcaa
    12961 acagctattt gcagcagaga aatttgtgct actccaacat gaccagaaca atcagcttaa
    13021 tatttagcaa catttggtga ttaagtttta tgcatttaat gaaaaaaatc tgaggaaaag
    13081 gtggatatct gagcgattaa taccatgaac gctaaaacgc acagcggaaa atgccagcgc
    13141 cagccataag taacgcaaaa aaagatttaa gcaaatataa aaaaagacaa tggtttctgt
    13201 cacaaatccg ttaatggata gtgagatatg gggcgcaaat ttggcaggga aatgtgtggt
    13261 gtgtaggtcg aataaggcgt tcacgccgca tccgacaata agtgcggtgc cgttgcctga
    13321 tgcgacgctg acgcgtctta tcaggcctac gtcgagtcga acctccctgc ccccggaggt
    13381 tctcttcctc atgggctgca gatgcaaaaa agcctgctcg ttgagcaggc ttttcgaatt
    13441 tggtcggtga tagaggattc gaacctccga ccccttcgtc ccgaacgaag tgcgctacca
    13501 ggctgcgcca atcaccgaat gcggggcgca tcttactgcg cagatacgcc ctcgtcaatc
    13561 ccttaatagc aaaatgcctt ttgatcggcg agaaagtcag caggcctctt agttagccac
    13621 tgcctctttt gtctgcggga tgtgacacca gttgttgttt tcgttaattc caccatccgg
    13681 tgaagtatag ccaagacaac ccatgatggt gtcgtacagc tctacgtgac ggcgtggcac
    13741 tttcatgtcg gctgcttttt tcagctgcgc aaacgcctgc gcattgaccg gattttccag
    13801 atatttatct gacatccaga ccatcatcgg tacgcggaac tgctccggcg gtgccagttc
    13861 acgcggcgtg ccgtgcaggt gctcgcgttc attaattgac tcgccgtggt cagctgcgta
    13921 gaacacaatc gctttcttat cgcgaacctg atcaatcaca ctggagataa agtgatccac
    13981 ataggtcacc gagttgtcat aggagttgat catctgcgct ttggtacatc cgctatcaac
    14041 accaatacat tccggcttcc actgcgcaaa gctgcgcggg taacgctggg tgtagttaaa
    14101 gtgcgaacct ttggtatgca gaatgattag atgcttacca tccgggttgc gccccagcga
    14161 ttgctgcatt tcgtctacca gcaacatatc atctaccggc ttgccacggt tacgtggctc
    14221 cgcaccaatc tgttcacggt aagcaatatt gtccgccatc gtattgctgt agaaccacat
    14281 ttcgctctgc atagcgtaga ggtcagaact gaatcctaac tgcttcagta ccgcgaaaat
    14341 gttctgttct tttaatgtgc gctgcggatt atcttccgcg cccccctgac gtacaaacat
    14401 gcaacgcaga gagagtttgg ttgcggtatc acaggagtaa ccacggaacg ccgccagatt
    14461 tttctcctgg gccagcttcg gcgtggtatt acgctcatag ccgaaaatgc ccatatggtc
    14521 ccaacgcgta gtttcaccga tgataaacac gacataagtg tcattaacat tttgcggtgc
    14581 ctgataagtg aatttcttcg ccgggttaag caatgaatta ttatcggaag attcatcgac
    14641 ccgcgcccag gcgtacagcc ccaacgcaga aagccagttt gatggcagat aagagttcgc
    14701 tacgacaccg ccataactcg gcaaatcaac gccggtcgcc ctctccactt tcttctgctg
    14761 gatatccagc aaacgaatcg gtgcccaaac cataattccc gccagtacga cgaccgccag
    14821 gctgcgaata cgctgccctg gggttcgcag ttgtcggagc aaggtgtagc gacagcggtt
    14881 attccagata aggataagag gcaatgcgct aacggcgatt aaccagagaa taaagttcag
    14941 accaacaact tcttttgaca ggtcgatatc ggtggtcatg acggaagcga tgatgccata
    15001 accaatgacc acattaagga aggtcatgta atagctggca cctgcggaaa agagcacaac
    15061 cagcgatgcc agaatacgcc aactgcggcg gccaaacagc gaaagaagac gtagtaaaaa
    15121 gaaggtcacc agtacggtgg cggccagttc aacaacagca gaaatgcctt tccagacggt
    15181 aaaatcgtgc gcatagctgc cgaagcggcg gtaaaaaacc gcgccattca taaaaaggcc
    15241 aatatagatt gcaagcaaaa agctcagctt ctgctgtgta atcgatttga tgtatctcat
    15301 gcaaacaaac cctggacaaa caggtgataa caccctgctt agcggctttt caggcccagc
    15361 aggggaaaag tgcggaggta gtcagataag taaaatctac acgcgcgaat aagtagacca
    15421 cagcaaaggg aaaaagtgtg gggaaagagt gtgcatgaag cacaaattta caaaagtgca
    15481 agtccggaat agaccatttt aacggattaa cgactattca gagataggtt gggccaggat
    15541 gctggcccag taacttactc ataggtgatg gaaaaattga caagggcatt cgctgtgcca
    15601 ccagtggcct gagtgttgac gaccacataa ctcgcctggt agtgaagcga cgtggtttcg
    15661 ccatcaatcg cccattcaat cggcgttccg ctggagcctt tgggaacttg ctgcgttcct
    15721 ttatcgtcct ctatcttcaa cgcaacaccc gttgcgccac cagcctcaag ggcaaacatg
    15781 gaatcatcat cgctgtccga ctgactgtcc atctcaacgg tcgctttgga tatcccatcg
    15841 cactctttga gttcaatggt gaaatctttc ttgctcaaca ccgtaccgac tgccgcaggt
    15901 ttttgcagcg tcattgttcc catatcgact tcgagatcgc ttttttccat ctcgcaagct
    15961 ttagtggtga tagtgatgga gaaatgaata tcccctgccg ggccaagatc cgtcgccgca
    16021 ttcaccccgc aggcaaacag actcaaactg gcgatgccgc tatgcagcaa tagattgaat
    16081 ttcatggcat cactccattt caagatatat ggtcgttacg ccttcaaacg tcccgccttt
    16141 aggcgcttta cccgtcgtgc taatcggtaa agccagcagt cgaatcgcgg cattgcggtg
    16201 agttttagaa tccaggctgc tcaaatttaa taacgtggtg ctgcttgcat cattcggtac
    16261 caaaattttg ccgctggaat ctttcaccag tacgccaata tcggggttgc ccatatccac
    16321 cgctcgggga tcgtcagcat tggttgcccc ctcaaggcgc aacgagactt tgataccatc
    16381 cgagatattg ttgcaatcaa agccgaggtt aatttcttgc tctgtcgcac cttcaggcat
    16441 ttgcccttgt ctgtttttaa acgctgagga ggggaattca ccaaatggca catccagcac
    16501 cgtgccgggc tttatttcgc aatcctgagt tatggtgacg cggccctgaa tagatacgga
    16561 agatatgggt attgttccgt aatctttatt tgttttggag gcatacagtt cggcaattaa
    16621 tgtcagaggg atgacggaat cgccgataaa gggtttagta atagcaaagg aaagtagacc
    16681 tgcgccgccc atatagggcg tagtaatgac tttgttgcat ccggttgttt gcagcgcctg
    16741 ctggtttttg aaaggaacaa atccatacac actggtactg atgcgaattt tcgtcgcaac
    16801 ctccagttcc tccgtcaaag tataatattt catactgtca accgaacggc cagaaggggg
    16861 aagcatactc accccttgta ctagagtatc tttttcattc ttataagaat caggacattc
    16921 acaaattagc gatacgtttt gtccattaga ccagttaagg acatccggta aaaccgttcc
    16981 tgcgacgttt tttgccgcat caggtatacc aacatccaca tcgacattat agtgataagt
    17041 cgtgccatcc gcgggggtac atggccccca cgcatctccg gcaaatgcct tgccagaaaa
    17101 tccgaatacg catcctaata aggataatgc tattgccgct ttcatttcgc tctccttgct
    17161 tactggcaat tggccgaaac aggaataatt ccgccctgtt ttaattccag ggataattta
    17221 taaggcgcca cgcattgctg cgacgcgtca ttaccccatt taatcaatac ccgtccttcc
    17281 tctggcattc cgctaatata gagctgtcct tcttcaccaa cgatactgct cacctcgttt
    17341 tgtttatcgg tgattagcgt tgccgttgct ccaaagggca ccgggctgcc cgcggaacga
    17401 agcagcgtca ttaatacgcg gtaaccaacg cgggtatcaa aacgcgctct gacaatcgct
    17461 ccacgcgttg gcaccacttt ctggaacgca ttttcaacgt caacatcgtc acccaacgaa
    17521 tcgctacgca gtgagatttc gttttcacga tacggcatgg cgtaaggcac gatcgcgtta
    17581 cccatccagt caacctgaac gccgccgtta ttcaccacgc gggcattgcg cgcaccgggt
    17641 acggagatga gcgtcatggt ttcgcctagc ggttgggaaa gcgtcacgcc ttcgctatga
    17701 actatcacgc cgccgcgaac gctgtagttg agctgccggt aatctttccc gtagctatag
    17761 cctacgttga cattaccgga cccaccctga tagccaacgt tagcgttacc gctgttaccc
    17821 acaccgttgc tggcgtagcc ttcctgaaca ctgtagctca ggttgcgatc ctcaagcagc
    17881 gttccgctga ccccaacctg ttgattggta cgaccatcct ggtcggtcgt gacgcgatag
    17941 ttactccagg cccggcctag tggaacggaa atattgaaag accacaagcg atcgctttca
    18001 tcccattcag ggcttttatt ccagctatat gcaatactgt aactgacctt gccaatacgt
    18061 ccgttgtaac ccgccgatac cgtgttctgt ttacctgcgt cgttccagta atcctgctgc
    18121 gttaaattta aataaacaga gccataggcc cctaattgct gcgtcaggtt accctgtatt
    18181 tcgctgcgct tgtgataacg gttatagtcg ctgtcagcgt cactgcgcac atcggtggct
    18241 tcctggaagg tatagaaacc gctggtagag taccgatagc ccgcaaggcg gaaatcggtg
    18301 ccgctttcga agctcttgga atataagaaa cgataagatt gtccctggct atcgcgatcg
    18361 ttattcagtt cgcttttagc ctgcgtcaca tcaatggaaa tcgccccgat ataaccaaag
    18421 tttttcccta tccctaatgc aaatgcattg taattattag agattaatac gccgccgtag
    18481 gccgtcatcc cccacggtaa accgtagatc agatcaagct gcccgaaacg cggcgaggcg
    18541 ctgtcataat tcccggcctg atattcacct gcggcaaagc tatatttgag atggccttca
    18601 cgctggaaaa ttggcaccga tgagaatggc tgaataaagc gttgttcact gccatccgct
    18661 tctttaataa tgaccgtgag atcaccgctg tttgcagtgg ggtaaagatc gtttatttca
    18721 aaggcaccag gctgcacata ggtgcgataa ataacgtagc cgttttgttc aatactgact
    18781 tcggcgttgc ttttggcgat ccctcgaacg atgggtgcaa agcctcgctg gctgtcgggc
    18841 agcatctctt catcagaggc taacatcgcg ccacgcatct gaacgctgtc aaaaatatcc
    18901 cccgcggtcg ccgtatcccc catcgttaat tgcgctttga aagggatgat ggccctactc
    18961 aaagacgtac cgatattatc ccactgcgct ttgccaccgg aatggctcca ggtactgtaa
    19021 ttacgtaacc gccacgcgcc aaggttcagg ccgctgcgta agttgaggta gtagctgtcg
    19081 ctctttagcg tatcgttgcc atcatcatga tgaacggtgc cattatcatc gacataactg
    19141 ctggaagaac ccgaggagtc atattcactg ttgctcccgc taaaactgta atccagcaat
    19201 aatgccggga taccttcgtc ccataaagat tccggcaccg tgccgcgcgc cacctggtgc
    19261 atcgccgcct gagggaaact taataccagc ttttgttcac tgaagtcgaa atggcttgag
    19321 gcctggggaa tgatttcgtc aaacggcacg caggcttcgg caggcaccag attgagggcc
    19381 ggaaagcttt caacgcgcac gcccatctct gccagttgcg tccggctaag gcaaggcaca
    19441 agggattccc ccatcgcttt gcgctgtgct tctgtggcct ctttaaattc cagtgtgcgg
    19501 gtttccattc tctgttcatt gacgtaaaca gagacacgat agatgccggg taactgctgt
    19561 tcctggcctt tttcgaacat cgttaaatcg acatgctggc ccacaccctg aacgtcctcc
    19621 aggaatttgg ggttaaactt tccggcatgt gcagaaaacg tgctggcaac agcaaccgcc
    19681 accacagaga tagaaaagga agaaaaagta cgtgaaacgg tcttccgtga cattgagcaa
    19741 tttctcctga aaaggtatta atcaatcaac atttttagtc atcatttccg ctgacgatcc
    19801 aaagtcattg atggcgttat atttaatctt cgaaacgcta
//
LOCUS       DAZGBC010000089        19581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGBC010000089 DAZGBC010000000
VERSION     DAZGBC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.089"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            222..722
                     /gene="tssB"
                     /locus_tag="ACHK1J_003047"
     CDS             222..722
                     /gene="tssB"
                     /locus_tag="ACHK1J_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7912287.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            757..981
                     /locus_tag="ACHK1J_003048"
     CDS             757..981
                     /locus_tag="ACHK1J_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912288.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1032..2507
                     /gene="tssC"
                     /locus_tag="ACHK1J_003049"
     CDS             1032..2507
                     /gene="tssC"
                     /locus_tag="ACHK1J_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7912289.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2514..2927
                     /gene="tssE"
                     /locus_tag="ACHK1J_003050"
     CDS             2514..2927
                     /gene="tssE"
                     /locus_tag="ACHK1J_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7912290.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2931..4781
                     /gene="tssF"
                     /locus_tag="ACHK1J_003051"
     CDS             2931..4781
                     /gene="tssF"
                     /locus_tag="ACHK1J_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7912291.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTMGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4745..5827
                     /gene="tssG"
                     /locus_tag="ACHK1J_003052"
     CDS             4745..5827
                     /gene="tssG"
                     /locus_tag="ACHK1J_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7912292.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALIRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5852..7132
                     /gene="tagH"
                     /locus_tag="ACHK1J_003053"
     CDS             5852..7132
                     /gene="tagH"
                     /locus_tag="ACHK1J_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO7912293.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7129..7653
                     /gene="tssJ"
                     /locus_tag="ACHK1J_003054"
     CDS             7129..7653
                     /gene="tssJ"
                     /locus_tag="ACHK1J_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7912294.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7656..8987
                     /gene="tssK"
                     /locus_tag="ACHK1J_003055"
     CDS             7656..8987
                     /gene="tssK"
                     /locus_tag="ACHK1J_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7912295.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8992..9753
                     /gene="icmH"
                     /locus_tag="ACHK1J_003056"
     CDS             8992..9753
                     /gene="icmH"
                     /locus_tag="ACHK1J_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO7912296.1"
                     /translation="MDEGSLSLPPFTGYDEKGQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILYQLNKLLR"
     gene            9762..12536
                     /gene="tssH"
                     /locus_tag="ACHK1J_003057"
                     /gene_synonym="clpV"
     CDS             9762..12536
                     /gene="tssH"
                     /locus_tag="ACHK1J_003057"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO7912297.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIDMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYILPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSVVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEVAWHQQQTLVREIIALRQQLLGMAEDT
                     DAVVDTSSESESESESESESESEQDNTAAIPADDAGSVQPEETAETVSPVQRLAQLTA
                     ELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGDTIKGQ
                     DLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTT
                     INMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNL
                     FYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPEIMQEALYPVLADFF
                     KPALLARMEVVPYLPLSKETLATIIAGKLTRLDNVLRSRFGAEVIIEPEVTDEIMSRV
                     TRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDAQDD
                     QSVTEDEPVL"
     gene            12533..13276
                     /gene="vasI"
                     /locus_tag="ACHK1J_003058"
     CDS             12533..13276
                     /gene="vasI"
                     /locus_tag="ACHK1J_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO7912298.1"
                     /translation="MSRMRVFRQTGWFIAGLMAGLPASAAPAETSSMAGVAVAVATAT
                     PLDAAATLQAMQSCRRESAALERLDCYDRLLEPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13281..14696
                     /gene="tssA"
                     /locus_tag="ACHK1J_003059"
     CDS             13281..14696
                     /gene="tssA"
                     /locus_tag="ACHK1J_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7912299.1"
                     /translation="MAMDLRDPNVWISHLLENLPDEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSPQTTATSESGVTQPSTPTPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMTLWQQVEKSVLLAPYWLDGHCISAQTAHRLGYTAVADAIR
                     DEVVSFLGRLPALADLLFNDHTPFVSEKTKQWLATQPGNQAAPVIQVSNEDIQAARLC
                     FDEQGLEAALRYLDTLPAGEPRDQFHRQYFGAQLMEEAGLVQLAQQQYRMLFRMGLQM
                     MVADWEPSLLEQLEQKFTAEQ"
     gene            14805..18239
                     /gene="tssM"
                     /locus_tag="ACHK1J_003060"
     CDS             14805..18239
                     /gene="tssM"
                     /locus_tag="ACHK1J_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO7912300.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVQVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPSVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18250..>19581
                     /locus_tag="ACHK1J_003061"
     CDS             18250..>19581
                     /locus_tag="ACHK1J_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO7912301.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRIQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQPSSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQNSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLNNNTIPTIKK"
BASE COUNT         4540 a         5413 c         5458 g         4170 t
ORIGIN      
        1 gaattgcaca ggttcataca atctatttaa cgcttgcaga atacccctct ttacgatcga
       61 taaaaagtcc ttttcttatt tattttgaag cgtaaaatgc ggggtgatga aggagtggtg
      121 attttatttt caccgccatc aaggcggcat ttccgatatt ttttgtggta cgaatatgcc
      181 gtgttcatgg cctgtacgag ataacatgac ggatcacttt tatgagcaaa aaatttgaag
      241 gcagcgtcgc gcctcgcgaa cgtattaata tatcgtatgt cccaaaaaca gatggccaga
      301 cggctgaagt ggaacttccg cttaacatgc tcgttgtcgg cgataccgga aatacgcagg
      361 aaacatcctc actggatgaa cgtcaggcag tttccgttaa caagcacaat ttcggtgcag
      421 tgatggctga agcagccatt ggcctcaatt ttaccgtccc cgccacgctg aagggaagca
      481 caacggacga tgagctgaat gtggcgctga atatcaagtc actggatgat ttttcaccag
      541 atagcgtggc ccggcaggtt ccggaagtga ataaattgct ggaactgcgt gaagcactga
      601 ctgcactgaa ggggccgatg ggtaaccttc cggcattccg tacccagctc caggcattgc
      661 tggaaaatga agagtcccgc gagcagttgc tcaaagaaat cggccaggtg agcaataaat
      721 aaccaccact atttcattaa acagggttat ccaggcatga cggcacacaa aaatatctcc
      781 gggacatacc acctctctgt tgcagacatt ctgcaggtgg tttaccaggt atgtttcagc
      841 ccgtccgttg aaatcaacca ggatggtgtg gctgcgctga ttacaacact ggatcgccgt
      901 atcagtgatc tattggatga gattatccat ttttgtgagt ttcagcagag cgcgtcgcac
      961 tggcagcgcg tacttcactg acggcgaaaa gccggtttca gaaacacagc taaaacagaa
     1021 ggattttcag tatgtcttta caggaagagg aactcgtttc cagtcatgcc gggcagcctg
     1081 agcaggcatc gtcgctgctt gatcaaatca tggcacagac ccgtattcag cccggttcgg
     1141 aaggttatga cgttgcccgc cagggtgtga cagcctttat tgccagcatc ctgcaatcca
     1201 ctgcatctgc cgaaccggtc aataaactgg ccgtggacag catgattgca gatattgatg
     1261 aacgcatcag ccgccagatg gatgtcatca tccatgcgcc tgcctttcag caggtcgagt
     1321 cgttctggcg ctcactgaaa accatggtgg atcgcgttga tttccgtgaa aacatcaagg
     1381 tcaacgtgct tcatgtcacc aagcaggaac ttctggaaga ctttgagttt gcgccagaaa
     1441 ttattcagtc cggattttat aagcacgttt attcgtccgg tttcggacag tttggcggtg
     1501 aacccatcgc tgccgtgctg ggcgcttatg agttcaaaaa taccgcgcct gacatgaaac
     1561 tgctgcaata cgtcagtgcc gtgggcgcaa tggcgcacgc accgttcctg tcctccgttt
     1621 ccccggagtt tatggggctg aactcgtgga ccgaactgcc taatatcaaa gatctttatg
     1681 ccatctttga aggtccggcc tacaccaaat ggcgcgccct gcgtgactcg gaagattcgc
     1741 gctatttagg gcttacggca ccccgcttcc tgcttcgcca gccctattca ccaacagata
     1801 accctgttaa gaactttaac tattacgaag atgtcagcca gaaccacgaa gattatctgt
     1861 ggggtaatac ggcctggatg ctggcatgca atatcgcaga cagttttgcc aaataccgct
     1921 ggtgtccaaa cattatcggt ccgcaaagcg gcggcgcagt gaaagatctg ccggtgcatc
     1981 tgttcgaaac gatggggcag attcaggcca aaatcccaac cgaggtgctg gtcaccgatc
     2041 gccgcgaatt tgaactggct gaagagggtt ttatcaccct taccatgcgt aaagactctg
     2101 ataatgcagc ctttttctct gcaaactcgg tacaaaaacc gaagcacttt ccgggaaaag
     2161 atgcagaaac caactataag ctgggtacgc agcttcctta tctcttcatc atcaatcggt
     2221 tagcgcacta catcaaggtg ttgcagcgtg aacaactggg gtcatggaaa gaaaggagcg
     2281 acttagaaag agagcttaat acctggatcc gtcagtatgt tgccgaccag gaaaacccgc
     2341 ctgcagacgt gcgcagccgc aaacctctgc gcgctgccaa agttgaggtt atggatgtag
     2401 aaggcgaacc cggctggtac caggttgcgc taagcgtgag acctcatttt aaatttatgg
     2461 gggcaaattt tgagctttcc ctggttggcc ggttagacag ggagtaacac accatgatac
     2521 cggatcggga aagaaccaca ggttctcttt ttgagcgtat ggaagcctct tcggccagaa
     2581 accggcaggg aggcagtatt cattcgctca ggcaatccat tcgccagaac ctacgaaaca
     2641 ttcttaatac ccggtccggt agttgccggg gtgcgcctga gctgggcatt gatgaaccgg
     2701 aaggggctga aaacttccgc gaatccatga gtcgggccat cgagcaatgt atagaacgct
     2761 atgaaccccg gatctcacac gctgaagtgc aggcagtggt ttcatccgcc tccagcccgc
     2821 tggatatgac gtttcatatt acagcctggg tgacatttaa cgaaacgcat gaggtgcttg
     2881 aatttgatat ggcaccgaat ggcagccagc attaccgggt ggactgacaa atggaatttg
     2941 aagaacgcta ttttcgggaa gaactcgatt acctgcgcca gcttagcaag ctgctggcaa
     3001 cggaaaaacc ccatctggcc cgcttcctgg ccgaaaaaga tgcggatccg gatattgaac
     3061 gcctgctgga aggggtggct tttcttaccg gcaatctccg ccagaaaatt gaggatgaat
     3121 tcccagaact gacgcacggg cttattaaga tgctatggcc taattacctg cgtccggttc
     3181 cggcaatgac ccttattgaa tatacgccgg atatggataa gtcttctgta ccggtgttaa
     3241 tcccccgtaa tgagcagttt acaaccaacg ccggggaaat cagagttgat gaagtgctgc
     3301 cctctgatgc taaaaaggag gagccgcctc cctgtacctt caccctctgc cgggatatct
     3361 ggctgctgcc cgttcgcctg gagcagattg aaaaccgcag tacgacccgt aatggtgtta
     3421 tcaacatcac cttttcggtc gcaccgggaa cggacttccg cacgctggat ctgaacaaac
     3481 ttcgcttctg gctcggcaat gacgacaact atacccgtga ccagctttat ttatggttct
     3541 gcgaatactt gcagggtgcc gacctgacta tgggtgaaca gcatattcgc ctgcctgagt
     3601 ttatgctaaa agctgtcggt tttgagccgc aggatgccat gctgccctgg ccgaaaaacg
     3661 tccacagcgg ctaccggatc cttcaggagt atttctgtta ccccgatgcg tttctctttt
     3721 ttgatctttg tggttgtccg gctttgcctg acggattgca ggcggaattc tttaccctgc
     3781 aactgcgttt ttcgcgccct ttgcccgtgg acatccggct gcgccgcgat tccctgcgcc
     3841 tgtattgcgc acctgccatt aatttattta tccaccatgc agaagccatc acgctggaca
     3901 accggcgggc agactatccg ctggttccca gccgccatta cccacaacat tacgatgtat
     3961 tttccgttaa cagtgtggtg agccaggtcc aggatatgtt caggaaaaaa gatctggggc
     4021 gtcctgtttc gacgcaggcc gcgcgccagt ggccagcctt tgaaagtttc agccatcaga
     4081 tggaatacag ccggaagcgg gaagtggtgt actggcatca ccggaccaaa acatccctgt
     4141 tccatcgcgg ctttgatcat acccttgcct ttatacatgc tgatggcagt tatccgtcag
     4201 acgaatctct gctcagtaat gaagtggttt cggtatcgct gacctgtacc aaccgtgagc
     4261 ttccgtcaca aattcgttcc ggcgatatca ccggcacaac cggtaaaaat gcggctgttg
     4321 cttcctttcg caacattacc cgcccgacgc aaccactctg gccggtcatt gatggcagcc
     4381 tgcactggtc cctgctctcc gccatgaacc tgaattatct gtcattactg gatacggacg
     4441 cgctgaagca ggtcatcgct aactttgatc gccacgcaat ccaccatccg cagacggcgc
     4501 ggctgtcaca acaaaagctg gatgccattg agcgtctgga gacccgcccc gttgatcgcc
     4561 tgtttacggg tattcccgtc cgggggctgg cctccacgct gtatctgcac ccggagccgt
     4621 ttgtctgtga aggggaaatg tatctgctcg gtacggtgct ttcgcatttt ctgtcgctgt
     4681 acgccagcgt taactcattc cacatgctga ccgttgtgaa cacagaaagc caggagacat
     4741 ggaaatggac ggaaagaatc gggcagcatc ctcttatctg agtccgggaa atcccccggc
     4801 agataaagag cagaatgatc cgctggcaca ggttttccat aatgcctgct cttacaattt
     4861 ttttgcgatg gcggagctgt tgcaccgcct ggcaaagggt gaaaagggga cgccagaatt
     4921 atccctgcgc gacgatccgg cacaggaaac cctgcgtttc agcgccgatg ccagccttgc
     4981 cttcccgtgc agtgatatca gtgcgctgat acgggatacg tcaggggcat tcaggatgac
     5041 caccaccttt atggggttac aggggagcca gtcgcctctc cccggttatt acctcgatca
     5101 cctggcctgg aaagcggtac atgaacaaag tccggtaggt gattttctgg atatgttcag
     5161 ccaccgcctg acccagtttg tctggcatat ctggcgtaag taccgctatc acatcagttt
     5221 tcgtaacggc ggtgtggatg cgttctcgca gcgcatgtac tcccttgtcg ggctgggcca
     5281 ccgccagctt cgcgataagc tggcgattaa tcacagcaaa atgctggcgt attccgggat
     5341 cctggcaaat ccgggacgat ccccggaaat tatctgtggc ctggtgtccc actgctttga
     5401 tttgtcagaa gtgacgctgc aaaactggca acgtcgcaag gtggatattg aaccggatca
     5461 gcaaaatagt ctggggagct attctcttaa aaatggcgaa aaactggcgg gaagatcggt
     5521 actgggcaac tttgttctgg gtacacgagt gcccgatctc agcggaaaat tccagctcag
     5581 tattaccagc ctgacccgaa aacagttcct ctcttttctg ccgtccggcg aaaacttcct
     5641 gccactgacg atgttcgtgt ccttcatcct gcgcgatcag cttgcctggg acttacatct
     5701 ggggctggcg ccggagcagg tgggcgcaat gcgcctgggc gataacaaaa gtgcgctgct
     5761 gggctggacc agcttcctcg gcacaccgga agaacgaccc tcagtcacca tcagggtacg
     5821 gtcgtaaatt atgataatca cggagcagaa tatgccggaa gagaaactac agacgctttc
     5881 attgcaggtc attaacggca gtgagctgga aagcgggcgg gcggcgcgct gtctgttcac
     5941 acagcaggga aatgtcggcc atggccccga atgccactgg tcggtacagg atcgtcagca
     6001 gagcattccg gcccaggctt ttaccgttat cctgcacgat ggcacatttt gtctacgccc
     6061 gcagacggca caactgtggc tgaatcaggc aaaagtcaca gcaacatcag acctgataca
     6121 gttgcgccag ggcgatgaga tccagatcgg acggctgatg gtgagggttc atctgaaccg
     6181 gggagatatt ccccattacg atgaggaaat ggccactccc gaaaccatcg ttaccaatcg
     6241 cgatatgctc acggataccc tgctatcaac ggagggtgcg ccacactatc cgggaatgac
     6301 tcaccggcac cagcttgcag acaccgtggt aaacggtttt tctgccgatc cactccaggc
     6361 acttcagtcc gaaagcctga ttaccacggg cgatccgctt tcaggcattg cggctgtccg
     6421 gccatcggca ccgctgtccg atccggcaag taatgggggg atcaatactc cgtttatgga
     6481 tctgccgccc atttatgcca gccctggcga tcataatgat gacatctctg cggcagaaat
     6541 ggcgcaacgc caccttgcgg tcaccccctt actgcgcggt cttggcggct cgcttaccgt
     6601 gagcaattcc gacgatgcgg atgattttct ggaggaggcc ggacgaacgt tacaggccgc
     6661 aataaaaggt ctgctcgatt tgcagcagca gcgtaacagc ctctcagaca aacatttgcg
     6721 cccgctggaa gataacccgc tgcgcctgaa catggattac gccaccgcgc tcgacgtgat
     6781 gtttgccgaa ggtaaaagcc cggtacatct ggcggctccc gccgccgtca gtgaaagcct
     6841 gcgcaatgtc cgccaccacg aagaagctaa ccgggcggcg attgtggagt cgcttcgtgt
     6901 cctgctggac gccttctcac cacaaaatct gctgcgccgc tttgtgcagt accgccgcag
     6961 ccatgaactg cgccagccgc tggatgatgc cggagcatgg caaatgtaca gccattatta
     7021 cgaagaactg gcctccgatc gccagcaggg gtttgagatg ctgtttaacg aggtctacgc
     7081 ccaggtctat gaccgggtgc ttcgtgaaaa acagcgggag ccggaagcat gaacaggaag
     7141 gcgtttctgg cctgcgtgct gatgtgcgtc atattaaccg gctgcgaaac ggcgaaaaaa
     7201 atcagcgagg tgatcaaaaa cccggatatc caggtcggga gcctgaagga acaaccttcg
     7261 gaaataaccg tcaccctgct gactgaaccg gacaccaata ccaatgcgga gggtgaatca
     7321 gccgccgtgg atgtgcaact ggtgtacatg actgacgact caaaattgca ggcagccgac
     7381 tatgaccaga tagccagtac gccgctaccg gatgtgctgg gtaaaaacta tatcgaccac
     7441 caggatttca acctgttgcc ggacaccatc aaaaccctgc cgccggtaaa gctggatgag
     7501 aaaacgcagt ttatcggtgt cgtggcctat ttctccgatg accaggccac tgaatggaaa
     7561 caaatcgaga cggtggaagg aaccggccac cattaccgcc tgctggtgca tgtacgtcaa
     7621 agcagcatcg aaatgaaaaa agaggatgaa taattatggc aaccacccgc aacaaggtga
     7681 tgtggcagga agggatgctg atgcgcccac accatttcca gcaacagcag cgttacaacg
     7741 actacctgga taaccagcgt ttccgggcca tgaatgattt atcctgggga tttaccgaac
     7801 tcaccctcaa caatgaactg ctggcgcagg gtaagatcat gattgacagc gcgtcaggca
     7861 cactgcctga cggcaccgtc ttttctatcc ccgaccagga cgcactgccc gatccgctgc
     7921 acccgcaaaa ttttccggac gaaagaagcc gcaatattta cctcgctctg ccggtcgcca
     7981 gtgatgtgag aaatgaaatc agcgacgggc ggcgaatcgg gcgttaccgg ctgaattatg
     8041 ccgatgtccg ggatctgcat tcagaagaag gcgacgcgcg aacactgacg ctgggacaac
     8101 tgacgccgcg cattatgagc ggtgcagaag atatgagtgc ctatattacg ctcccgcttt
     8161 gccgtatcag cgatcgccat gccgacggct ccctgacgct ggatgacgat tttatcccct
     8221 cctgccagaa tattcaggtc agtaagaaac tgcgtgttta tctcaaagag gtacaggggg
     8281 ccattggcgg acgggcaagc gatctggcaa accgcattgg ctctccggcg cagagcggca
     8341 tcgcggatgt ggcggaattt atgatgttgc agttgcttaa ccgtaaccag acccggttta
     8401 cccatcgcgc ccgccgatcc cagctccacc cggaagattt ctaccttgat cttgccgggt
     8461 tgctgggtga actgatgacc tttaccgagc cgtcgcgcct gccctgcccg cttgatgtgt
     8521 atgatcatca tgacctgacc aaaacattta aaacactgtt accggaagtc aaacgggcgc
     8581 tgcataccgt actgtcgcca agagcggtca atctgccgct gcatctgcgt gacggcatct
     8641 ggcaggccga tgttcatgac tcggaactgc tacagtctgc cacctttgta ctggccgtgg
     8701 cggcaaatat gcctgtcgat cagatccagc gtcagtttat ccagcagtcg aaaatttcct
     8761 cgccggaaaa aatccgcaat atggtcagtg tgcagatccc cggcattcca ttgcgtgccc
     8821 tgatggtggc tccccgtcag cttccttacc attccgggtt cagctatttc gaactcgaca
     8881 agagcggaca ggcctggaca gaaatggctg ccgccggggc cgttgcactg catgtatccg
     8941 gcagtttccc ggatctgaac atgcaactgt gggcgataag agggtaagac gatggatgag
     9001 gggtcactgt ctctgccacc atttaccgga tacgacgaaa aaggccagcg cgactaccat
     9061 ctcgcgcttc gtggcaacag cctgaatccg atgatcgacg cagccacacc gctactcggt
     9121 atggtaatgc gcctttccac catgaacagc cagacgatgc cggagcatct ttttgctcag
     9181 gtggtgacgg atgttcaggc cgtggagcaa ctgcttcagg aacagggcta cgaaccgggc
     9241 gtgattatct cgtttcgcta catcctctgc acgtttattg atgaagcggc gctcggtaac
     9301 ggctggtcga acaagaacga gtggatcaag cagtcattgc tggtccattt ccacaacgaa
     9361 gcctggggtg gcgagaaagt tttcatcctg ctggagcgcc tgatccgtga acccaaacgt
     9421 taccaggatc tgctggaatt tctctggctc tgtttttctc tgggatttcg cgggcgctac
     9481 aaagtggcag cacaggatca gggggaattt gagcagatct accgccgtct gtaccacgtt
     9541 ctgcataaac tgcgcggcga tgcccctttc ccgctgttac accaggacaa aaaaacccag
     9601 ggcgggcgtt atcagctcat cagtcgcctg accattaaac atattttttg tggtggcgtt
     9661 gtcgtgctgg cgttgtttta cctgttctat ctgttgcggc tggacagcca gacgcaggac
     9721 atcctgtacc agctaaacaa attacttcga taaggacgtt tatgatccag attgatcttc
     9781 ccacgctggt aaaacggctg aacctgttct cccgccaggc gctggagatg gccgcctcag
     9841 aatgtatgag ccagcaggca gcggaaatta ccgtcagcca tgtgcttatt cagatgctcg
     9901 ccatgccacg cagtgacctg cgggttatca ctcggcaggg cgatattgac atggaagagt
     9961 tgcgccaggc gctgacggtg gagaactaca caaccgcccg ttctgcggac agctacccgg
    10021 cgttttcccc gatgctggtt gagtggctga aagagggctg gctgctggcg tcggctgaga
    10081 tgcagcacag cgaactgcgc ggcggcgtgt tgctgctggc cctgctgcat tcgccgctgc
    10141 gttatatact gcctgctgcc gcccggctgt tgaccggtat taaccgtgac cgtctgcaac
    10201 aggactttgt gcagtggaca caggagtcgg cggaatcagt cgtgccggat gcagacggta
    10261 aaggcgcagg cacactgacg gacgcctctg acaccctgct tgcccgctac gccaaaaaca
    10321 tgaccgaaga tgcccgtaac ggcaggcttg acccggtact gtgccgcgac cacgaaatcg
    10381 acctgatgat cgacattctc tgccgccgcc gtaaaaacaa cccggtggtg gtgggcgaag
    10441 cgggcgtggg caaaagcgcg ctgattgaag gtctggcgct gcgcatcgtg gcaggtcagg
    10501 tgccggacaa gctgaaaaac accgatatca tgacccttga cctgggcgca ttgcaggccg
    10561 gggcatcggt gaagggcgaa ttcgaaaaac gtttcaaggg gctgatggcg gaggtcattt
    10621 cctctccggt gccggtcatt ctgtttatcg acgaagcgca taccctgatt ggcgcgggca
    10681 accagcaggg cgggctggat atctccaacc tgctcaaacc ggcgctggcg cgcggcgagc
    10741 tgaaaaccat cgccgccacc acctggagcg agtacaaaaa atacttcgag aaagacgccg
    10801 ccctgtcgcg ccgcttccag ttggtgaagg tcagcgaacc caacgccgcc gaagccacca
    10861 ttattctgcg tggtctgtcg gtagtgtatg aacagtctca cggcgtgctg attgatgatg
    10921 acgccttgca ggccgcagca acgctgagcg agcgttatct ctccgggcgt cagttgccgg
    10981 acaaagcgat tgatgtgctg gataccgcct gcgcccgtgt ggccatcaac ctgtcatcgc
    11041 caccaaagca aatctcggcg ctgaccaccc tgagccacca gcaggaggcg gaaattcgcc
    11101 agcttgagcg cgagcttcgc atcgggctgc gtaccgacac ctcacggatg accgaggtgc
    11161 tggtgcagta tgatgaaacg ctgacggcgc tggatgaact ggaagtcgcc tggcatcagc
    11221 agcagacgct ggtacgggaa attatcgcgc ttcgccagca gttactgggt atggcagagg
    11281 acacagatgc cgtagtggat acgtcgtcag agtcagagtc agagtcagag tcagagtcag
    11341 agtcagagtc agaacaggat aataccgctg ccataccggc tgatgatgct ggcagcgtac
    11401 agccggaaga aaccgctgaa acagtttccc cggtacagcg actggcacag cttactgccg
    11461 aactggacgc cctgcataac gaccggttgc tggtttcccc gcacgtcgat aaaaaacaga
    11521 ttgcggcggt gattgccgaa tggaccggcg taccgcttaa ccgcctgtcg cagaatgaaa
    11581 tgtcggtcat caccgacctg ccagtatggc tgggcgacac catcaaaggc caggatctgg
    11641 cgattgccag cctgcacaaa cacctgctga ctgcacgcgc cgacctgcgt cgtccgggac
    11701 gtccgcttgg cgcgttcctg ctggctggcc ccagcggtgt gggtaaaacc gaaaccgtcc
    11761 tgcaactggc ggagctgctc tatggcggtc gccagtacct gaccaccatc aatatgtccg
    11821 agtttcagga gaaacacact gtctcgcggc tgattggttc ccctcctggc tatgtcggct
    11881 atggcgaagg cggcgtactg accgaagcca tccgccagaa accgtactcg gtggtactgc
    11941 tcgatgaagt ggaaaaagcg caccctgatg tgctcaacct gttctaccag gcgttcgaca
    12001 agggcgagat ggcagacggc gaaggccgcc tgattgactg taagaatatc gtcttcttcc
    12061 tcacgtccaa cctcggctac caggtgattg tcgaacacgc ggatgacccc gagatcatgc
    12121 aggaagccct ctatccggtg ctggcggact tctttaaacc tgccctgctg gcgcgaatgg
    12181 aagtggtgcc gtacctgccg ctgtcgaaag agacgctcgc caccatcatt gccgggaaac
    12241 tcacccgtct ggataacgtg ctgcgcagcc gctttggcgc agaagtgatt atagagccgg
    12301 aagtgacgga cgaaatcatg agccgtgtca cccgcgcgga aaacggcgca aggatgctcg
    12361 aatcggtcat cgatggcgac atgctgccgc cgctctcgct gctgctgttg cagaaaatgg
    12421 cggccaatac ggcgattgcc cgtatccgcc tgtcggcggc agacggcgcg ttcacggcag
    12481 atgtggaaga tgctcaggac gaccaatccg tcacagagga tgaaccggtt ttatgagcag
    12541 gatgcgcgtt ttccggcaaa caggatggtt tatcgcgggc ctgatggcgg gcttacccgc
    12601 cagtgccgcg cccgcagaaa catcgtcaat ggccggtgtg gcggtagccg ttgccacagc
    12661 gaccccgctg gatgcggcag ccacgttaca ggccatgcag tcctgtcggc gggagtctgc
    12721 ggcgctggag cggcttgact gctatgaccg cctgctggaa ccgctgtccc cgtcagggtt
    12781 tgacggggcg ctggtcaaag ctggttttgt gggcgaagcc tggacccgcg ccacagagca
    12841 ggaaaaacgc cgccagggca acaccacaga gctgctggtg acgcaggtgc cgggggaacg
    12901 cccgacggtg gtgataacca ccccggctat cggccatgtt ccgccacgcc cggtgctgat
    12961 gttcagttgc gtggacaata ttacccggat gcaggtggcg ctgatgcacc ctctggatgt
    13021 ccatgatatc gccgtcaccc tgaatgccga cagccgggca ttacgcagcc actggtttgt
    13081 gcgcgagaac ggcaccctgc tggagtccag ccgggggtta tccggcattg atgaaattaa
    13141 gcaactgttc ggcgcaaaaa cgctgacggt ggatactggc gcagacaacg ccgccggaaa
    13201 gctgaccttt aacattgacg ggctagcaag ggcgattgct cccctgcgcg atgcctgcca
    13261 ctgggcggga gaataaaaat atggcaatgg acttacgcga tccgaatgtc tggatatcgc
    13321 acctgctgga aaacctgccg gatgagaaac tggcatcggc gttgaaagac gacaacccgg
    13381 actgggagta tatcgacggc gaaatcgtca aactggggtc actggcccac agccagctgg
    13441 atatcccgga actccagcgc cgggggctgg tcattctggc ttcggagagt aaggattttc
    13501 gtctgctggc gcatctgctg agaaccctgc aacacgccgg tgatcctcat ctcgcattgc
    13561 gcctgctggc gctgtatgtg gagcattact ggtcggtggc tgcgccgcag aatatggcac
    13621 ataaaaaacg ctttgccagc caggtcatta aacgctttga aacaggtatt gaaggctttt
    13681 cacagaacgc cgccacagcg cagcgcgacg cgctgtcagg ggaactggcg aaactggcgc
    13741 agtgctggca gtcacataac gccccggaac tggcactggc taccgatgat ctttttgccc
    13801 tgtaccagcg tgcgtttaat cgtgcggcac cggctcctgc ccccactccg gcggcctccg
    13861 atagttcacc acagaccacc gccacgtctg aaagcggcgt gacgcaaccc agtactccga
    13921 ctccccaaat cgccatcgac agtcacgacg acaaagcctg gcgcgacacg ctgttaaaag
    13981 tggcggctat tttatgtgaa cgccagccgg actcgccgca gggctatcgc ctgcgccgcc
    14041 atgccctgtg gcaatccatc accagtacac cccaggcgga aagcgatgga cgtaccccat
    14101 tggctgcggt ctctgccgat atggtggcgg attaccagtc ccggcttgcc agcgcggata
    14161 tgacgctgtg gcagcaggtg gaaaaaagcg tactgctggc accctactgg ctggacggtc
    14221 actgcatttc tgcacagacg gcgcaccgtc ttggctatac cgctgtggcc gatgccatcc
    14281 gcgatgaagt cgtttccttt cttggccgtc tgcccgcgct cgctgatctt ctgtttaacg
    14341 accacacacc gtttgtttct gaaaaaacga agcaatggct tgcaactcag ccaggtaacc
    14401 aggccgcgcc agtcatacag gtgagtaatg aagatattca ggctgcgcgg ttgtgttttg
    14461 atgaacaggg gctggaagcc gccttacggt atctggatac gttgcccgcc ggtgaacccc
    14521 gcgaccagtt tcaccggcag tattttggcg cgcagcttat ggaagaggcg gggctggtac
    14581 agcttgcgca gcagcagtac cggatgttgt tcaggatggg attgcagatg atggtggcag
    14641 actgggagcc gtccctgctt gagcagctag aacaaaaatt cacggcagaa caataagcga
    14701 taacaggagt ttttgtgttc agattaccaa caccccgact actcagcgga ctcaaatcag
    14761 ccctacgccc ggcgatgccc cggtttaaag tctccgcttt ctggctgctg atactggcgt
    14821 ggatttttct gctggtatgg atctggtgga aaggcccgac gtggacgctg tatgaagaac
    14881 agtggctcaa accactggcg aaccgctggc tggcaacggc ggcatggggg attatcgccc
    14941 tggtgtggct taccgtccag gtgatgaagc gcctgcaaca acttgaaaaa atgcaaaagc
    15001 agcagcgcga ggaggccatt gatccgctca gtgtggagct gaacgcccag cagcgttacc
    15061 ttgaccgctg gctgctgcgc ctgcaacgcc atctcgacaa ccgccgtttc ctgtggcagt
    15121 tgccgtggta tatggtcatc ggcccggcgg gcagtggcaa aacgacactg ctgcgcgagg
    15181 ggtttccgtc agacattatt tatgccccgg agggcgcgcg cggcacagaa caacgcctgt
    15241 accttacgcc ccatgtcggt aaacaggcgg ttatctttga tatcgacggc acactctgcg
    15301 ctcccgctga tgcggatatc ctgcatcgcc gtctgtggga acatgccctc ggctggctga
    15361 aagaaaagcg cgcgcgccag ccgctgaacg ggattattct gacactcgat ttacccgatc
    15421 tgcttaccgc agacaaacgc cgccgcgagc atctgttaca gacgctgcgc agccgcttgc
    15481 aggatatacg ccagcatctt cactgccagt taccggttta cgtggtactc acccggcttg
    15541 atttattgca gggttttgcc gccctgttcc agtcactgaa cagacaggat cgcgatgcga
    15601 ttctgggcgt cacgttcacc cgccgcgccc atgaaaatga tgactggcga acagagttga
    15661 atgctttctg gcagacatgg gtggatcgaa tgaacctagc gttgccggat ctgatggtcg
    15721 ctcagactca cacccgcgcg tctttattta gtttttcccg ccagatgcag ggaagccgtg
    15781 aaccgctggt gtcactgctt gagggtctgc ttgatggcga aaatatgaac gtgatgctgc
    15841 gtggtgtcta tctcacctct tcgcttcagc gtggacagat ggatgatata ttcacccagt
    15901 ctgccgcccg ccagtaccgg ctgggcaata acccactggc ttcctggcct ctggtggaca
    15961 ccgcgcctta tttcacccgc agcttgttcc cgcaggcatt actcgcagag cctaatctgg
    16021 caacggagag ccgcgcctgg ctgatgcgtt cccgtcgccg cctgacggtt ttctctacca
    16081 caggaggcgt ggcggcactg ctgctcatca ccggctggca tcactattac aacggtaact
    16141 atcagtccgg catcaccgtg cttaagcagg ccaaagcctt tatggacgtg ccgcctccgc
    16201 agggggaaga tgactacggc aacctgcaac tgccgctgct gaacccggta cgtgatgcca
    16261 cactggccta tggcgactgg ggcgaccgca gccgtctggc cgatatggga ctgtaccagg
    16321 ggcgacgtat cgggccatat gtggaacaga cctatctgca actgctggag caacgttacc
    16381 taccctcgct gtttaacggg ctggttaaag cgatgaatgc cgcgccgccg gagagtgaag
    16441 aaaaactcgc ggtgctgcgc gtgatgcgaa tgctggagga caaaagcgga cgtaacaatg
    16501 aggtggtgaa gcagtatatg gcaaaacgct ggagcgaaaa gttccacggc cagcgcgata
    16561 tccaggcaca actgatgtcc catcttgact acgcgctggc tcatactgac tggcacgcag
    16621 agcgtcaggc gggcgacggt gacgccatca gccgctggac gccatatgac aagcccgtgg
    16681 tatcagcaca gaaagaactg agcaaactgc ctgtctacca gcgggtttac cagagcctga
    16741 aaacgcgggc gctgggcgtt cttcctgccg acctcaatct gcgtgaccag gtagggccaa
    16801 cctttgatca ggtatttaca tctgccgatg acaacaaact ggttgttcca cagtttctta
    16861 cccgttacgg cctgcaaagc tattttgtaa aacagcgcga tgaactggtt gaactgacgg
    16921 cgatggattc ctgggtactt aaccttaccc gcagcgtgaa atacagtgac gccgaccgcg
    16981 cggaaatcca gcgccagctc accgagcagt atatcagcga ctacaccgcc acctggcggg
    17041 ccgggatgga caatctgaat atccgcaatt ttgagtccat cggacaactg accggggcgc
    17101 tggagcaggt tatcagcggc gaccagcctt tgcagcgggc gctgaccgtg ctgcgtgaca
    17161 acacacagcc aagcgtcttt tctgaaaaac tctctgccaa agaacgggat gaggccctgg
    17221 cagagccgga ctaccagtta ctcacccgtc tcgggcatga gttcgccccg gaaaacagta
    17281 ccctggcagt acagaaagac aaagaaagca cgatgcaggc agtatatcag caactcaccg
    17341 agttgcaccg ctatctgctg gcgatccaga acgcgcctgt accagggaaa tcggcgctga
    17401 aagccgtgca gttacggctt gatcagaaca gcagcgatcc gatattcgcc acccgccaga
    17461 tggcaaaaac gctgcctgct ccgctcaacc gctgggttgg caggctggct gaccaggcct
    17521 ggcatgtggt gatggtggag gccgttcact atatggaagt ggactggcgc gacagcgtgg
    17581 tgaaaccgtt taacgagcaa ctggcaaata actatccgtt taatccgcgt tctgcacagg
    17641 atgcctcact ggatgccttc gaacgcttct ttaaaccgga tggcatactg gataccttct
    17701 accagcagaa cctgaagctg tttatcgata atgacctgag tctggaggat ggcgataaca
    17761 acgtcattat tcgcgaagat attattgcgc aactggaaac tgcgcagaaa atccgtgaca
    17821 tcttcttcag caaacagaac ggtctgggaa catcctttgc cgtggaaacg gtatcgcttt
    17881 caggcaataa acgccgcagt gtactgaacc ttgacggtca gttagtcgat tacagccagg
    17941 gccgtaacta taccgcccat ctggtctggc ctaacaacat gcgcgaaggc aacgaaagta
    18001 agctgacgct catcggcacc agcggcaacg cgccgcgcag tatcagcttc agcgggccgt
    18061 gggcgcagtt ccgcctgttc ggggccggac aactgaccgg agtacaggat ggcaacttta
    18121 ccgtgcgctt tagcgtggac ggtggcgcga tgacctaccg tgtgcatacc gacacggaag
    18181 ataacccgtt cagcggtggg ttgttcagcc agtttggtct gtcagacaca ctgtactgag
    18241 gaggcaggga tgaatagtaa cgtactgaca caaactatcg ttaccggcag tgacccgcgc
    18301 gggctgccgg aattcagcgc catccgcgag gaaataaaca aagccagcca cccgtcacag
    18361 cctgagctga actggaaact ggtggagtcg ctggcgctgg cgatttttaa agccaacggt
    18421 gtggatttac acaccgccac ctactatacg cttgcccgga cacggataca gggactggcg
    18481 ggattctgcg aaggtgcgga actgctggcg gcaatggtaa gccacgactg ggataagttc
    18541 tggccgcagg gcggcccggc gcgtactgaa atgctggact ggtttaactc ccgaaccggc
    18601 aatattctgc gtcagcaaat ctcctttgcg gaatctgatc tgccgctgat ctaccgcaca
    18661 gagcgggcat tgcagcttat ctgcgacaag cttcagcagg tagagctgaa gcgcgttccg
    18721 cgcgtggaga atctgctcta ttttatgcag aacacgcgta aacggcttga accccagctg
    18781 aagagtaaca ctgagaacgc cgcacagacc acggtcagaa cgctgattta tgccccggaa
    18841 acacagccat cttccacacc agaagcggta gtgcctcccc tgcccggcct gcctgagatg
    18901 aaagtggaag tgcgcagtct gacagagaat cccccacagg ccagtgtgat aaagcaaggc
    18961 agtacggtaa gagggtttat cgcagggatc gcctgttcag tggctgtcgc ctcagcattg
    19021 tggtggtggc aggtctatcc ggtgcagcag caactgttac aggttaacga caccgctcag
    19081 ggcgcagcaa cggtgtggat ggcctcacct gaactcgaaa actatgagcg caggctgcaa
    19141 caacttcttg atacctcccc ggtacagccg ctggaaaccg ggatgcagat gatgcgtgtt
    19201 gccgacagtc gctggccgga aagcctgcaa cagcaacagg cctcgacaca atggaatgag
    19261 gcactcaaaa cccgcgcaca gaatagcccg cagttgcgtg gctggttgca gacccgccag
    19321 gacttacatg cttttgcaga tctagtgatg cagcgcgaga aagagggact aaccctttcc
    19381 tatatcaaaa atgtcatctg gcaggcggag cggggactgg ggcaggaaac acccgttgag
    19441 tctctgttga cgcagtacca ggatgcccgt gcgcagaagc agaatacaga tgcgctggaa
    19501 aaacaaatta atgagcgact cgaaggcgtg ttaagccgct ggctgctgct gaataataat
    19561 acgataccga cgataaaaaa a
//
LOCUS       DAZGBC010000090        19222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGBC010000090 DAZGBC010000000
VERSION     DAZGBC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.090"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(223..1872)
                     /gene="pgi"
                     /locus_tag="ACHK1J_003062"
     CDS             complement(223..1872)
                     /gene="pgi"
                     /locus_tag="ACHK1J_003062"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO7912302.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2109..2305
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2397..3746
                     /gene="lysC"
                     /locus_tag="ACHK1J_003063"
     CDS             2397..3746
                     /gene="lysC"
                     /locus_tag="ACHK1J_003063"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO7912303.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLSMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3999..4271
                     /locus_tag="ACHK1J_003064"
     CDS             3999..4271
                     /locus_tag="ACHK1J_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO7912304.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4272..5144)
                     /gene="rluF"
                     /locus_tag="ACHK1J_003065"
     CDS             complement(4272..5144)
                     /gene="rluF"
                     /locus_tag="ACHK1J_003065"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO7912305.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQDNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKTKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5492..6439
                     /locus_tag="ACHK1J_003066"
     CDS             5492..6439
                     /locus_tag="ACHK1J_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO7912306.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6509..7315
                     /locus_tag="ACHK1J_003067"
     CDS             6509..7315
                     /locus_tag="ACHK1J_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7912307.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7325..7732
                     /locus_tag="ACHK1J_003068"
     CDS             7325..7732
                     /locus_tag="ACHK1J_003068"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO7912308.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNTNVEAVEFVPGENAGDIV
                     AKLKKLVSIHSQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7732..8226
                     /locus_tag="ACHK1J_003069"
     CDS             7732..8226
                     /locus_tag="ACHK1J_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO7912309.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8292..9089
                     /locus_tag="ACHK1J_003070"
     CDS             8292..9089
                     /locus_tag="ACHK1J_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO7912310.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9100..9924
                     /locus_tag="ACHK1J_003071"
     CDS             9100..9924
                     /locus_tag="ACHK1J_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO7912311.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9976..11205
                     /gene="sorE"
                     /locus_tag="ACHK1J_003072"
     CDS             9976..11205
                     /gene="sorE"
                     /locus_tag="ACHK1J_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO7912312.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSMVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSESQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11587..12276
                     /gene="pepE"
                     /locus_tag="ACHK1J_003073"
     CDS             11587..12276
                     /gene="pepE"
                     /locus_tag="ACHK1J_003073"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO7912313.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12367..13998)
                     /locus_tag="ACHK1J_003074"
     CDS             complement(12367..13998)
                     /locus_tag="ACHK1J_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO7912314.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14218..17901)
                     /gene="metH"
                     /locus_tag="ACHK1J_003075"
     CDS             complement(14218..17901)
                     /gene="metH"
                     /locus_tag="ACHK1J_003075"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO7912315.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRVRKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLETARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18101..18925
                     /gene="iclR"
                     /locus_tag="ACHK1J_003076"
     CDS             18101..18925
                     /gene="iclR"
                     /locus_tag="ACHK1J_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO7912316.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4554 a         5008 c         5021 g         4639 t
ORIGIN      
        1 acaaggagat ttatctgata aaacaatgcc gactttaagt cggcattgtt attaaggtta
       61 tttaattaat cgtgctatgt attaattgcc gaatacgatg tacatatcgg catcgacctg
      121 taggcctgat aagacgcgac cgcgtcgcat caggcatcgg ttgccggatg cggcgtgaac
      181 gccttatccg gcctacatag cggcatcagg catgaacgat gattaaccgc gccacgcttt
      241 atagcggtta atcagaccat tggtcgagct atcgtggctg ctgatttctt tatcatcttt
      301 cagctctggc agaatacggt tcgccagctg tttacccagt tccacgcccc actggtcgaa
      361 ggtgaagatg ttcaggatca cgccctgagt aaagattttg tgctcataca gcgcaatcaa
      421 cgcacccagg ctgaacggag tgatttcacg cagcaggatg gagttggtcg ggcggttacc
      481 ttcgaacact ttgaacggca ccacgtagtc aagcgttgcc ggatctttac cctgatcgcg
      541 atattcctgc tcaaccactt cgcgggattt accaaacgcc agcgcttcgg tctgagcaaa
      601 gaagttagac agcagtttct ggtgatgatc agagagcggg ttatgggtga tagccggggc
      661 gatgaaatcg cacggtacca ttttggttcc ctgatggatc agctggtaga acgcgtgctg
      721 accgttagtg cctggttcac cccagataat cgggccagtc tggtaatcca caacgttacc
      781 gttacggtca acatacttac cgttggactc catattgccc tgctggaagt acgccgcaaa
      841 acggtgcata tactggtcat acggcagaat cgcttcagtt tccgcaccaa agaaattgtt
      901 gtaccagatg ccaatcagcg ccagcaagac aggcaggttt ttctcggcag gcgtggtgga
      961 gaaatgcttg tccatcgcgt gtgcgccaga aagcagttca acgaagttat caaagccgat
     1021 ggagagaaca atcgacaggc caatcgctga ccacaaagag taacgaccgc cgacccagtc
     1081 ccagaactcg aacatgttgg cagtatcaat accaaactcg ccaacggctt tggcattggt
     1141 ggagagcgcc gcaaagtgtt tcgcaacgtg tttttcatca cctgccgctt tcaggaacca
     1201 gtcacgcgcg ctatgggcgt tggtcatggt ttcctgagtg gtgaaggttt tagatgctac
     1261 caggaacagg gtagtttccg ggtttacttt tttcagcact tccgcgatgt gggtgccatc
     1321 gacgttagac acaaagtgca tgttcaggtg gtttttgtac ggacgcagtg cttcggtcac
     1381 catgtacggg ccgaggtcag aaccgccgat cccgatgttc actacgtcag tgattgcttt
     1441 gccggtataa cccttccact caccggagat aatcgcttgt gagaaggttt tcatcttctc
     1501 cagcaccgcg ttgacttccg gcattacgtc tttgccatca accagaatcg gggtattgct
     1561 acggttacgc agcgctacgt gcagcacggc gcggttttca gtgcggttga tcttctcgcc
     1621 agagaacatc gacttaatcg cgcccgccag atcgcactct ttcgccaaat cttgtaattt
     1681 cgccagcgtc tcttcagtga tgcggttttt ggagtaatcc accagcatct gatcgtcgaa
     1741 ggttgcggag aacttagaaa aacgatcgcc gtctttagca aaaagatcgg cgatcgtaac
     1801 gtctttcatt tcatcgaagt gtttctgtag tgcctgccag gcagcggtct gcgttggatt
     1861 gatgtttttc attagcaata ctcttctgat tttgagaatt gtgactttgg aagattgtag
     1921 cgccagtcac agaaaaatgt gatggtttta gtgccgttag cgtaatgttg agtgtaaacc
     1981 cttagcgcag tgaagcattt attagctgaa ctactgaccg ccaggagtgg atgaaaaatc
     2041 cgcatgaccc cgtcgttgac aaccgccccg ctcacccttt atttataaat gtactacctg
     2101 cgctagcgca ggccagaaga ggcgcgttgc ccaagtaacg gtgttggagg agccagtcct
     2161 gtgataacac ctgagggggt gcatcgccga ggtgattgaa cggctggcca cgttcatcat
     2221 cggctacagg ggctgaatcc cctgggttgt caccagaagc gttcgcagtc gggcgtttcg
     2281 caagtggtgg agcacttctg ggtgaaaata gtagcgaagt atcgctctgc gcccacccgt
     2341 cttccgctct tcccttgtgc caaggctgaa aatggatccc ctgacacgag gtagttatgt
     2401 ctgaaattgt tgtctccaaa tttggcggta ccagcgtagc tgattttgac gccatgaacc
     2461 gcagcgctga tattgtgctt tctgatgcca acgtacgttt agttgtcctc tcggcttctg
     2521 ctggtatcac taatctgctg gtcgctttag ctgaaggact ggaacctggc gagcgattcg
     2581 aaaaactcga cgctatccgc aacatccagt ttgccattct ggaacgtctg cgttacccga
     2641 acgttatccg tgaagagatt gaacgtctgc tggagaacat tactgttctg gcagaagcgg
     2701 cggcgctggc aacgtctccg gcgctgacag atgagctggt cagccacggc gagctgatgt
     2761 cgaccctgct gtttgttgag atcctgcgcg aacgcgatgt tcaggcacag tggtttgatg
     2821 tacgtaaagt gatgcgtacc aacgaccgat ttggtcgtgc agagccagat gtagccgcgc
     2881 tggcggaact ggccgcgctg cagctgctcc cacgtctcaa tgaaggctta gtgatcaccc
     2941 agggatttat cggtagcgaa aataaaggtc gtacaacgac gcttggccgt ggaggcagcg
     3001 attatacggc agccttgctg gcggaggctt tacacgcatc tcgtgttgat atctggaccg
     3061 acgtcccggg catctacacc accgatccac gcgtagtttc cgcagcaaaa cgcattgatg
     3121 aaatcgcgtt tgccgaagcg gcagagatgg caacttttgg tgcaaaagta ctgcatccgg
     3181 caacgttgct acccgcagta cgcagcgata tcccggtctt tgtcggctcc agcaaagacc
     3241 cacgcgcagg tggtacgctg gtgtgcaata aaactgaaaa tccgccgctg ttccgcgcgc
     3301 tggcgcttcg tcgcaatcag actctgctca ctttgcacag cctgagtatg ctgcattctc
     3361 gcggtttcct cgcggaagtt ttcggcatcc tcgcgcggca taatatttcg gtagacttaa
     3421 tcaccacgtc agaagtgagc gtggcattaa cccttgatac caccggttca acctccactg
     3481 gcgatacgtt gctgacgcaa tctctgctga tggagctttc cgcactgtgc cgggtggagg
     3541 tggaagaagg tctggcgctg gtcgcgttga ttggcaatga cctgtcaaaa gcctgcggcg
     3601 ttggcaaaga ggtattcggc gtactggaac cgttcaacat tcgcatgatt tgttatggcg
     3661 catccagcca taacctgtgc ttcctggtgc ccggcgaaga tgccgagcag gtggtgcaga
     3721 agctgcattt taatttgttt gagtaaatac tgtatggcct ggaagctata tttcgggccg
     3781 tattgatttt cttgtcacta tgctcatcaa taaacgagcc tgtactctgt taaccagcgt
     3841 ctttatcgga gaataattgc ctttaatttt tttatctgca tctctaatta attatcgaaa
     3901 gagataaata gttaagagaa ggcaaaatga atattatcag ttctgctcgc aaacgaattc
     3961 ccgtacactg aactctctgg ctcactgagg gcaccatcat ggcactcccc cgtattaccc
     4021 aaaaagagat gactgaacgc gagcagcgtg aacttaaaac actgctggat cgggcgcgga
     4081 ttgcgcatgg tcgcgtactg accaactccg aaaccaacag cattaagaaa gagtacatcg
     4141 ataaattaat ggtcgagcgt gaggctgaag cgaaaaaagc ccgccagtta aagaaaaagc
     4201 aggcttataa accggatcct gaagcgtcat ttagctggtc agcaaatacg tcgacgcgcg
     4261 gcaggcgctg attagcgccc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
     4321 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
     4381 cccgccgttt tcggtttggc cttcgtttta ggttttgcct cggaagagga attttcgatg
     4441 agcttaaaga gatcgataag ctcgtcgtcg gttaaatcgc gccattctcc cagcggaatg
     4501 ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
     4561 tgctcgcaca tgcgacggat ctgacggttc agcccctgca ccagggtaat gcggaagaca
     4621 aacggcgctt cttttttgac tttacacttt ttagtcactg tcccgaggat tggcaccccc
     4681 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
     4741 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
     4801 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
     4861 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aatgcctacg
     4921 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     4981 tttactttca caacgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     5041 aggaacacat tgtcttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     5101 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     5161 cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt
     5221 aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacaa tgcaaatttg
     5281 cgtggcaaaa taaggataat caacctatta cgaatctccg cacacctgtg ctgataacta
     5341 ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa
     5401 aatcgctttg aaaggccatc atgatggggt tatatcagat caagcggtga gaaagcgtat
     5461 ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat
     5521 tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga
     5581 actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat
     5641 tgtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa
     5701 gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac
     5761 tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg
     5821 cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca
     5881 ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact
     5941 cgaaagccgc tatcacgtaa acacattaac ctacagcgcg gctgcgaagc tgaaagggga
     6001 atcgcatctc gcggattttc cggctctgct ggataacccc ttaattcgta atgggatcat
     6061 gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggatg tcgccctggt
     6121 gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga
     6181 agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga
     6241 tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acactctcta tcgaaatgaa
     6301 taaattaaag caggcacgat attccattgg cattgccatg agtgaagaaa aatacagcgg
     6361 aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc
     6421 tgaactatta ctgaaataaa aatcttcccg atgtttatcg taataaaaat tacggcgggt
     6481 ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat
     6541 cattatcgtc accggcggcg catccggtat tggtctggcg attgtagagg aattattagc
     6601 acaaggcgca aacgtacaga tggtagatat tcacggtggc gatggtcaat atgaagttca
     6661 taaaggctat cagttttggc cgaccgatat ttccagcgcc aaagaggtaa atcatacggt
     6721 agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa
     6781 tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc
     6841 tgcattcgaa aaaatggtca atatcaacca gaaaggcgtt tttctgatgt cgcaggcggt
     6901 ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg
     6961 actggaaggc tcagaaggcc aaagctgtta cgccgctacc aaagccgcgc tcaatagctt
     7021 cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc
     7081 cgggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac
     7141 gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg
     7201 gcgcgccgga agattagcag aagtcgctga ttttgtttgt tatctgttgt ctgaacgcgc
     7261 cagctacatc accggagtaa ccactaacat tgcaggcggc aaaacgcgcg gataaggagg
     7321 ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc
     7381 ggttcagatg gtttacggca acaccaatgt tgaagccgtt gaatttgtgc ccggtgaaaa
     7441 cgccggagac atcgtagcaa agctgaaaaa gttagtaagc attcacagtc aagatgagtg
     7501 gctgattgcc gtggatttac agtgcggcag cccgtggaat gcggcagcga tgcttgcgat
     7561 gcgcaatcct cgtctgcggg taatcagcgg actttctctg ccgctggcgc tcgaactggt
     7621 tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa
     7681 gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt
     7741 acgctcgccc gcattgatga ccgcctgatc cacggtcagg tcactaccgt ctggtcgaaa
     7801 gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga tgaagttcgc
     7861 cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa
     7921 aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc
     7981 actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctaaat
     8041 attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat
     8101 gatgatgata ttaacgcatt tcatgagtta aataatcttg gcgtcatact ggatttacgc
     8161 gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca
     8221 aattaaataa tagcgcctgt gtacatatgc tttaacaggc agggatgacg acacttaaaa
     8281 ggtgacaaat catggaaata agtaccctac aaattattgc tatatttctt ttttcctgta
     8341 ttgccggaat gggcagcgtg ctggatgaat tccagactca tcgcccgtta attgcctgta
     8401 cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggt ggtacgctgg
     8461 aattgatagc tctcggctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg
     8521 ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg
     8581 ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgca cgtaccatca
     8641 ccgtagtgtt ccagcacgcg gcggataaag cagcggaaga agcgcggttt cgcactctcg
     8701 atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga
     8761 ttgtctcgct gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc attcccgaat
     8821 ttgtgacccg aggactacag attgctggcg gttttatcgt ggtggtcggt tacgccatgg
     8881 tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag
     8941 gtggctacct cgatctcagt ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg
     9001 tctacatcca gctaaatcca cagtggcgta aagctgaacc tcaactccag gccaccgcct
     9061 ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca
     9121 cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt
     9181 gaacgtattc acgggctggg tttttgctac gacatgatcc ccgccatcaa gcgactttac
     9241 ccattaaaag aggatcaggt tgcggcgctc aggcgacacc tggtgttctt caataccacg
     9301 ccagccgtat gtggcccggt catcggcgtc actgccgcca tggaagaggc gcgggccaac
     9361 ggcgcggaaa ttgatgacgg taccattaac ggcatcaaag tcggtctgat gggaccattg
     9421 gcaggggttg gcgatccact ggtctgggga acgctgcgcc cgattaccgc cgcgctcggc
     9481 gcatctctgg cactttcggg caacattctc ggcccgctgc tgttcttctt tattttcaac
     9541 gcggtgcgtc tggcgatgaa gtggtatggc ctacagctcg gctttcgcaa aggggtgaat
     9601 atcgtcagcg atatgggcgg gaatgtgctg caaaaactca ccgaaggcgc gtcgattctc
     9661 ggactgtttg tgatgggcgt gctggtcacc aaatggacgt caatcaacgt accgttggtg
     9721 gtttcacaaa cgcatgccgc cgatggctcc accgtcacca tgaccgtgca gaacattctc
     9781 gaccaacttt gccctggttt gctggcgctc ggtctgacgc tactaatggt tcgtctgctc
     9841 aacaaaaaaa ttaacccggt atggctgatt ttcgccctgt ttggcttagg gattatcggc
     9901 aatgcgctgg gcttcctgtc ctgattcttc gccccggcac gactgccggg gccatcgctc
     9961 aacatgaggt ggtttatgaa aacaacagct ctgcgtcttt atggtaaacg tgatttacgc
    10021 ctggaaacct ttgaccttcc tgaaatgcag gaggatgaaa tcctcgcgac ggtggtcact
    10081 gacagcctgt gcctctcttc ctggaaagag gccaacctgg gtgaaaacca taaaaaagta
    10141 cccgacgatg tggcgaccaa cccaatcatc atcggccacg agttttgcgg cgatattctg
    10201 gccgtgggta aaaagtggca gcacaaattc cagccgggtc agcgttatgt gattcaggcc
    10261 aacctgcaac tccccgaccg cccggactgc cccggctact ccttcccgtg ggtaggcggc
    10321 gaagccacgc atgtggttat tcccaacgag gtcatggaac aagattgcct gctggcatac
    10381 gacggcgaaa cctattttga aggctcgatg gttgaaccgc tttcctgcgt gattggcgcg
    10441 ttcaacgcca actatcatct tcaggaaggt agttataacc acacgatggg gattcgcccg
    10501 caagggcgca cgctgatcct cggcggcacc ggaccaatgg gactgttggc gattgattat
    10561 gcgctgcatg gacccgttaa cccgtcgctg ctcgtcatta ccgataccga caacgataaa
    10621 ttgagttatg cgcgcaagca ctatccatca gaatcgcaaa cactgattca ttatctcaat
    10681 gccaccgatg cagcatttga tacgctaatg gcgctgagtg gcggtcacgg cttcgatgat
    10741 attttcgtct ttgtgcctaa tgaaggactg gtgactctcg cctcttcctt gctggcgaca
    10801 gatggttgcc tgaatttctt cgccggaccg caggataaac atttcagcgc gccaattaat
    10861 ttctacgatg tgcattatgc atttacccac tacgtgggca cgtcaggcgg caataccgac
    10921 gacatgcgcg cggcggtcaa actgatcgaa gagaaaaaag tgcaggccgc aaaagtggta
    10981 acacatattc ttgggctgaa tgccgcgggc gaaaccacgc ttgaattgcc tgccgtcggc
    11041 ggcggcaaaa agctggtgta taccgggaaa tacctgccgc tgacgtcact cacgcagatt
    11101 caggatcaag cactggcggc gattctggcg cgtcatcagg ggatctggtc cggtgaggcg
    11161 gagcaatatc tgctcactca tgcagaggca atttcccatg attaatcgcg ttactcaact
    11221 ctgcatgtcg ctggctggtc gcaccagcaa cttcgcgacc ggatgtgctg ctgattgctg
    11281 aagcagcaca atttgcacga gaagcataaa aaatatcagc gccttaaggg gcgctcaaat
    11341 tggtggcaaa gtgcacattg tttatgccgg atgcggaatg aagccttatt cggtctacat
    11401 aattttgaaa attcaatata ttgcagttat ctggtaggcc aggtaagcgt agcgcatcag
    11461 gcaatgctgt gtttgtcatc agtctcagcg cccggtgcaa tacgtgacat cgtgagccat
    11521 gtcagtaagt ggttaaattc gcagtggtag cattccttcc gtgatttctt actaaccgga
    11581 gaagtaatgg aactgctttt attgagtaac tcgacgctgc cgggtaaagc ctggctggaa
    11641 catgcactgc cgctaattgc tgaacagttg cagggtcgcc gctcagcggt gtttatccct
    11701 ttcgctggcg taacgcagac ctgggatgat tacacagcga aaacggctgc ggttcttgct
    11761 ccgctgggtg tttctgtcac cggtattcat agcgttgtcg atcccgttgc cgcgattgaa
    11821 aatgctgaga tcgtgattgt cggcggcggg aatactttcc agttgctgaa acagtgccgc
    11881 gagcgcgggc tgctggcacc aattactgac gtggttaaac gtggcgcact gtatattggt
    11941 tggagcgcag gcgctaacct tgcttgccca accattcgta ccaccaacga tatgccgatt
    12001 gtcgatccgc aaggtttcga tgcgctgaat ctgttcccgc tgcaaatcaa cccgcacttc
    12061 accaacgcgc tgccggaagg ccataaaggt gaaacccgtg aacagcgtat tcgcgaactg
    12121 ctggtcgtcg cgccagaact cacaattatt ggtctaccgg aaggtaactg gatcacagtg
    12181 agtaaaggtc acgcaacgct gggtggcccg aacaccactt atgtgtttaa ggctggtgaa
    12241 gaagcggttc cgctagaagc tggtcaccgt ttttaagtga attgccggat gcatgatccg
    12301 gcatcttgtt catagtgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc
    12361 acaatattaa tactcgtccc gtccttcatc ttcatccggc tgttccagca cactgtaagc
    12421 caccgaacaa aacagcgagt tcaggcgctg catatctccc agtaagccta aatgtagcga
    12481 actggtttca atgctctgca cgttttgctg atgcaggcga tcgacgtggg cgtgggaata
    12541 gcggcgatta agaatacgaa agcgatgctt gctgcggcgc aaacgacggg cgctggtaac
    12601 atcaccagag aagaaaaccg acattgccag ttttaaatta ctgagcaatt gctcatagag
    12661 cgcatccagt tccttcaagc catcaagcga aaatgcccgc cgtgctgcca gcgatttatc
    12721 ggcaatttcg ctccccatgc gctcgacgat atcggaggcc tgttcaaggt tgagcgacat
    12781 ttcgatgatc tccgcccagc ggcgcgactc ttcttctgcc agttcctctt ttggcatccg
    12841 cgccagatac agcttaatgg cggtatagag aacgttgata tcatcagcca gcttacgcag
    12901 ctctttctcc tgccgtggct cgccgtgcat cactttattc agcccttcca tcatctgttc
    12961 catggcgtcg ccaatacgca gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt
    13021 atccagcgcg ctgacatcca gatgtttggg tcgtagctgg gtatccagtt ccggttcatc
    13081 gcgaataatc gttttgcaaa accgtgccat cgggtcaaca aatggcagca tggccaggca
    13141 acgtacaagg ttgtagaaga cgtggaaata gatcaccagt tccgcttttg gcaatgacag
    13201 cttccccatt gtctctgcca gcaaatggac aaacggcagg ataatcaggc tacccaccag
    13261 cttaaacagc agactaccca gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt
    13321 gagcatcgcc agcaggccgg aaccgaggtt agcaccaatc accagacaga gcgccacggg
    13381 gaaggagata atgcctgcgg cggtcagagt cgcagtcagc agtacagcag caaggctgga
    13441 gtagctgata atggcgaaca tcgcgccaat cagcgcatcc agcagaatat cgccggtcag
    13501 cgaggcaaag atcacctgaa cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat
    13561 caactccagc gccagcaaaa tcagcccaag accaataccg acgcggccca gttgcccggc
    13621 gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata aaaataagta acggtgacag
    13681 ccaggataag tcgaaggtga gaatacgcgc cattagcgcc gtcccgacat ctgcacccag
    13741 cacaatcacc agagccggtg cgagggctac cagatcctga gcgacgaacg aggtcaccag
    13801 catggtggtg gcattactgc tctgcaccag tgcggtaacg ccgatccccg cgcaaaaggc
    13861 gagcggcttc ttttcgacgc tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg
    13921 cattacgccg gttcgaacaa tatgagtccc ccagaccagc agggcgacgg cagaaagcag
    13981 gtgaagcagc gttaacacga gttcaggttc tccttatcgt tatatgttcc tgaagcaatt
    14041 gaatgaccaa aggaccggaa acacgctgac tggcatcttt actgtgcctt ttgacattcg
    14101 tttttccggc taagattatt ccagcctgtt tccagtataa gtgtttcata ttaataaaga
    14161 gacagggcac tcattgagcg ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca
    14221 gtccgcgtca taccccagat tcggtgccag ccagcgctca acatcggaga cgctcatacc
    14281 tttacggcgg gcatagtctt caacctgatc gcgctgaatt tgcgccaccg cgtagtactt
    14341 gctgtccgga tgactgaaat accaacccga aaccgatgca ccgggccaca tggcgaaaga
    14401 ttctgtgagt ttcatgccgg tatgtttttc cacttccagc agctcccaga tggtggcttt
    14461 ttccgtatgt tccgggcagg ccgggtagcc cggtgccgga cggatgccct ggtagttttc
    14521 gcggatcagt tcttcgttgc tgaggttctc gttcggcgca tagccccagt agactttacg
    14581 cacacgctca tggagatact ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac
    14641 catgattttg ttgtaatcat cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc
    14701 cagcccgcca gtcacggcaa atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc
    14761 aacgaagtca gcgagacagt agttagcgaa tcctgttttt tcggtctgtt gacgcagatg
    14821 gtggctgacg ttgatcacat gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc
    14881 cacacggttt gccgggaaca gccccaccac accacgcgga ttcagcgttt tctcggcgct
    14941 taatttatcc agcatgtcgt tggcgtcttt aaacagccgc tgcgcctcaa cgcctaccac
    15001 ttcatcttcc agaatgcgcg gatacttccc ggccagcgac caggtcataa agaacggcgt
    15061 ccagtcgatg taattacgca gcgtttcgat gctggcttca acttcctgca caccgagacg
    15121 gtgcgccacc ggcggcgtgt aagcctgcca gtcaaaagcg aaatcgttat cgcgcgccgt
    15181 ttccagcgtg accggtggtg tgcgtggttt cttgcgcccg tgctgaatac gcacggtttc
    15241 gtactccttg cgggtacgag cgacaaaatc atcgcgctga gtatcggaaa gcagcgccgc
    15301 caccacgcca acggtgcgcg aggcattctg cacatacacc gtcgggccgc tgtagttctg
    15361 ctcaattttc accgccgtgt gcgcttttga ggtggtcgcg ccgccaatca atagcggaat
    15421 ggtgaagccc tgacgctcca tctctttcgc cacgttaacc atctcgtcca gcgacggcgt
    15481 gataagcccc gaaaggccaa tcaaatcagc attcacttct ttagcagtac ggagaatttt
    15541 ttccgcaggc accatcacgc cgagatcgac aatttcgtag ttgttacatt gcagcaccac
    15601 gccaacgata tttttaccga tatcgtggac gtcgcccttc acggtggcga tcaccatctt
    15661 gccgttggtt ttgccctgct ctttgctggc ttcaataaac ggttcgagat acgccaccgc
    15721 ctgtttcatg acgcgcgccg atttgaccac ctgtggcagg aacattttcc cttcgccaaa
    15781 cagatcgccg accacgttca tgccgtccat caacgggcct tcaatcactt caatcgggcg
    15841 cgtagcctgc tggcgggctt cttcggtatc ctgctcgata aactcagtaa tgcctttgac
    15901 cagcgagtat tccagacgtt tattcacttc ccacgagcgc cactccgcct gctgggcgtt
    15961 agcggtgtcg tcggttttgc tgccgcgata tttctcggca agctccagta aacgctcagt
    16021 gccatcatcg cgacgattaa gaatcacatc ttccaccgcg tcgcgcagtt cagcgggcag
    16081 gtcgtcgtaa atcgccagtt gcccggcgtt gacgatcccc atatccatgc cattgcgaat
    16141 agcgtagtag aggaacaccg cgtgaatagc ttcgcgcacc ggatcgttac cacggaacga
    16201 gaacgagacg ttggagacgc cgccggaaat cagcgcgtgc ggcagttcgc gtttgatgtc
    16261 ttcacacgcg ccgataaagt cctgcgcata gttgttgtgc tcttcaatgc cggtggcgac
    16321 cgcgaagatg ttcgggtcga agatgatatc ttctggcggg aagccaacct cttcggtgag
    16381 gattttgtac gcccgacggc aaatctcgat tttccgtacg cgggtatcgg cctgtccctg
    16441 ttcgtcaaag gccattacca ccacggccgc accgtaacga cgcaacaatt tcgcgtgatg
    16501 gataaaggca tcgacgccct ctttcatcga gatagagtta acaatgcctt tgccctggat
    16561 acacttcaga cctttttcaa tgacatccca ttttgaggag tcgatcataa tcggcacgcg
    16621 agcgatatcc ggttcaccgg caatcagatt gagaaaacgc accatcgccg cttcggcatc
    16681 gagcatccct tcatccatgt tgatatcgat aatctgcgca ccgttttcca cctgctggcg
    16741 ggccacatcc agcgcctcgc tgtatttctc ttctttgatc aggcgcttga acttagcgga
    16801 accggtgacg ttagtccgct cgccgacgtt cacaaacagg ctgtcgtcgc caatgttcag
    16861 cggctccagg ccggacaaac ggcaggctac gggaatttca ggcagtttgc gcggcgctaa
    16921 tccttctact gcacgactca tcgctgcaat atgttgtggc gtggtgccac agcagccgcc
    16981 gacgatattg agaaaacccg cttgcgccca ttcacgtatc tgttttgcca tcgtgtcggc
    17041 gtcgagatcg tactcaccaa aggcgttggg tagcccggcg ttcgggtgcg cggtgacgta
    17101 gcattccgca atccgtgaca gctcctgcac gtactggcgc agttcatctg gccccagcgc
    17161 acagttcagg ccaaaggtca gcgcttcggc gtggcgcaat gagttgtaaa atgcttcggt
    17221 ggtctgcccg gagagcgtgc gcccggaggc gtcggtgatg gtgccggaga tcataatcgg
    17281 cagctcaacg cccagcgctt caaactccgt tttcaccgca aaaaccgccg ctttggcgtt
    17341 aagggtgtcg aaaacggttt caatcaggat cagatccgca ccaccttcca ccagcgcttt
    17401 ggtggattcg cgataggccg ccaccagctg gtcaaaagtg atgttgcgga atgccggatc
    17461 gttgacgtcc ggagaaatag acgccgtgcg gttggtcggg ccgagaacac cggcaacgta
    17521 gcgcggtttc tctggcgtgc gcgcggtcca ctcatcagca caggcgcgcg ccagtttcgc
    17581 cgccgcaaag ttgatttccg ccgacaggga ttccatctgg taatccgcca tcgcaatggt
    17641 cgtggagttg aaggtgttgg tttcgatgat atccgcgccc gcttcaaagt aggcgttgtg
    17701 gatagcggcg atcacttccg gtttgcttag caccagcaga tcgttattgc ctttgaggtc
    17761 gcatggccag tcggcaaagc gttcaccacg aaaatcggct tcgttcagtc gatagctctg
    17821 gatcatggtg cccataccgc cgtccagcac cagaatacgt tcatttaact gcgcacgcag
    17881 ttgttccact ttgctgctca cacttgctcc cgacacgctc aacccagatt taataaaaat
    17941 tcaacaaacc atactggcat aaacgcatct gtggtaaaag cgaccaccac gcaacatgag
    18001 atttgttcaa cattaactca tcggatcagt tcagcaacta ttgcattagc taacaataaa
    18061 aatgaaaatg atttccacga tacagaaaaa ggagactgtc atggtcgcac ccattcccgc
    18121 gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg actggacagg ttcagtcttt
    18181 aacgcgtggc ctgaaattac tggagtggat tgccgaatcc aatggcagtg tggcactcac
    18241 ggagctggcg caacaagccg ggttacccaa ttccacgacc caccgcctgc taaccacgat
    18301 gcaacagcag ggtttcgtgc gtcaggttgg cgaactggga cactgggcaa tcggcgcaca
    18361 tgcctttatg gtcggcagca gctttctcca gagccgtaat ttgttagcaa ttgttcaccc
    18421 tatcctgcgc aatctaatgg aagagtctgg cgaaacggtc aatatggcgg tgcttgatca
    18481 aagcgatcac gaagcgatta ttatcgacca ggtacagtgt acccacctga tgcgaatgtc
    18541 cgcgcctatc ggcggtaaat tacctatgca cgcttccggt gcgggtaaag cctttttagc
    18601 ccaactgagc gaagaacagg tgacgaagct gctgcaccgc aaagggttac atgcctatac
    18661 ccacgcaacg ctggtgtctc ctgtgcattt aaaagaagat ctcgcccaaa cgcgcaaacg
    18721 gggttattca tttgacgatg aggaacatgc gctggggcta cgttgccttg cagcgtgtat
    18781 tttcgatgag caccgtgaac cgtttgccgc aatttctatt tccggaccga tttcacgtat
    18841 taccgatgac cgcgtgaccg agtttggcgc gatggtgatt aaagcggcga aggaagtgac
    18901 gctggcgtac ggtggaatgc gctgactttt tctggcgggc agaggcaata ttctgcccgt
    18961 catacctgag tggtaataga ataagggtgt ctgttaatcg cattgacgcc aaaataactt
    19021 attgtcatac acttcacaca cttttattaa ctcagcatta tttttaaaca tcaaaccact
    19081 taattattaa caaaatcgca agatctcatt ccaccgaaac ttttacggct gctcaatgag
    19141 attgagcgat ttatgatatt tctttagttg taatcatatt aaattttttc actgttctga
    19201 tagttaaaat tcaagacatc aa
//
LOCUS       DAZGBC010000091        18929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGBC010000091 DAZGBC010000000
VERSION     DAZGBC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.091"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..908
                     /gene="focA"
                     /locus_tag="ACHK1J_003077"
     CDS             51..908
                     /gene="focA"
                     /locus_tag="ACHK1J_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO7912317.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            963..3245
                     /gene="pflB"
                     /locus_tag="ACHK1J_003078"
     CDS             963..3245
                     /gene="pflB"
                     /locus_tag="ACHK1J_003078"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7912318.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3437..4177
                     /gene="pflA"
                     /locus_tag="ACHK1J_003079"
     CDS             3437..4177
                     /gene="pflA"
                     /locus_tag="ACHK1J_003079"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO7912319.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            4276..4386
                     /locus_tag="ACHK1J_003080"
                     /pseudo
     CDS             4276..4386
                     /locus_tag="ACHK1J_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4387..5817)
                     /gene="ycaM"
                     /locus_tag="ACHK1J_003081"
     CDS             complement(4387..5817)
                     /gene="ycaM"
                     /locus_tag="ACHK1J_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7912320.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIHNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(6027..7175)
                     /gene="ycaD"
                     /locus_tag="ACHK1J_003082"
     CDS             complement(6027..7175)
                     /gene="ycaD"
                     /locus_tag="ACHK1J_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7912321.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            7490..8116
                     /gene="ycaC"
                     /locus_tag="ACHK1J_003083"
     CDS             7490..8116
                     /gene="ycaC"
                     /locus_tag="ACHK1J_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO7912322.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(8152..9015)
                     /gene="dmsC"
                     /locus_tag="ACHK1J_003084"
     CDS             complement(8152..9015)
                     /gene="dmsC"
                     /locus_tag="ACHK1J_003084"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO7912323.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9017..9634)
                     /gene="dmsB"
                     /locus_tag="ACHK1J_003085"
     CDS             complement(9017..9634)
                     /gene="dmsB"
                     /locus_tag="ACHK1J_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO7912324.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(9645..12089)
                     /gene="dmsA"
                     /locus_tag="ACHK1J_003086"
     CDS             complement(9645..12089)
                     /gene="dmsA"
                     /locus_tag="ACHK1J_003086"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO7912325.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(12389..13381)
                     /locus_tag="ACHK1J_003087"
     CDS             complement(12389..13381)
                     /locus_tag="ACHK1J_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7912326.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIEAWWLLDGRNQDYGDTYRVRLEKINREMGDPRASQMT
                     RKFMLGYRSDKLQAGLMPSSINRDLCALSAMFSSLIEGEVFHNENPVRGIRKLKVRNT
                     EMAFLSDEEIERLLERLDGDARRVAILCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDAVLALIKTRRTGLLYQADYLRFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLAESVHKLSID"
     gene            complement(13451..13792)
                     /locus_tag="ACHK1J_003088"
     CDS             complement(13451..13792)
                     /locus_tag="ACHK1J_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7912327.1"
                     /translation="MVILSIDYAEKLRQIRKAEGLTQKQFAEITGLSLATVRNYESGQ
                     KSARAKIVESVLQADRFEKYMFWLLKDKTLPAAGQIAPALSLDGSSQSEGDLASIDVT
                     QKSSRSGRNAG"
     gene            13896..14420
                     /locus_tag="ACHK1J_003089"
     CDS             13896..14420
                     /locus_tag="ACHK1J_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO7912328.1"
                     /translation="MRDFIADKELSDTDHTTGAAYINANTSQQEATDNNDEQKPQRTY
                     KKGATLRLDGPIAGLCSLEKGAAYIGLTKTALRAALQRNQMPGHKTRSNPEDENSDGV
                     WWFNVREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFTPADKEDCQTVNGKRVYKG
                     RKSKLEQSQDVNER"
     gene            14427..14822
                     /locus_tag="ACHK1J_003090"
     CDS             14427..14822
                     /locus_tag="ACHK1J_003090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912329.1"
                     /translation="MSGKNKSEKTATVLKQLSLLKEEHKDATILMHLEGSPKITITTT
                     YSELHKELEGLINFCSKLKANENIDDLILKPEAEFGHFCESRENGCIGIMLYASRGIM
                     INLPVEQAVDIFGEMALNKTTIYKRKMPE"
     gene            complement(14836..15030)
                     /locus_tag="ACHK1J_003091"
     CDS             complement(14836..15030)
                     /locus_tag="ACHK1J_003091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912330.1"
                     /translation="MSYDDELIKMLKSVIEIVDKIPEVPPSSDDKHDQIRDLANDVRE
                     KVLHKYPRKSSWNFSKGPTY"
     gene            15138..15476
                     /locus_tag="ACHK1J_003092"
     CDS             15138..15476
                     /locus_tag="ACHK1J_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO7912331.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNSHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRR"
     gene            15496..15738
                     /locus_tag="ACHK1J_003093"
     CDS             15496..15738
                     /locus_tag="ACHK1J_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO7912332.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIVEPYFTEVAA"
     gene            15735..15860
                     /locus_tag="ACHK1J_003094"
     CDS             15735..15860
                     /locus_tag="ACHK1J_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912333.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            15940..16218
                     /locus_tag="ACHK1J_003095"
     CDS             15940..16218
                     /locus_tag="ACHK1J_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912334.1"
                     /translation="MSINTPTEINKLFTDPAHIEFTQKTLTEITNVLDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELRTQSGQFSA"
     gene            16230..16472
                     /locus_tag="ACHK1J_003096"
     CDS             16230..16472
                     /locus_tag="ACHK1J_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912335.1"
                     /translation="MNSIFTATMLTRFTDAVGHEFMVESHLITTTTPCPSDADYLYIH
                     LADGTQITAIASTVREVTAIRGAWKSETQAHGELRP"
     gene            16469..16681
                     /locus_tag="ACHK1J_003097"
     CDS             16469..16681
                     /locus_tag="ACHK1J_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912336.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            16707..16973
                     /locus_tag="ACHK1J_003098"
     CDS             16707..16973
                     /locus_tag="ACHK1J_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO7912337.1"
                     /translation="MTILDYIAANPGCSGSEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLAHMFNQLLKEARA"
     gene            16970..17269
                     /locus_tag="ACHK1J_003099"
     CDS             16970..17269
                     /locus_tag="ACHK1J_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912338.1"
                     /translation="MSTINHQKLRELAFALKRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTAPAIRLTLHHEIADFCATLESPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            17592..17819
                     /locus_tag="ACHK1J_003100"
     CDS             17592..17819
                     /locus_tag="ACHK1J_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO7912339.1"
                     /translation="MANRKQQRARAERLHTRTEINRRLWRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            17888..18298
                     /locus_tag="ACHK1J_003101"
     CDS             17888..18298
                     /locus_tag="ACHK1J_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912340.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVEPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            18295..>18929
                     /locus_tag="ACHK1J_003102"
     CDS             18295..>18929
                     /locus_tag="ACHK1J_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7912341.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALERACD"
BASE COUNT         5095 a         4994 c         4498 g         4342 t
ORIGIN      
        1 ataatgcttt gttagtatct cgtcgccgac ttaataaaga gagagttagt gtgaaagctg
       61 acaacccttt tgatctttta cttcctgctg caatggccaa agtggccgaa gaggcgggtg
      121 tctataaagc aacgaaacat ccgcttaaga ctttctatct ggcgattacc gccggtgttt
      181 tcatctcaat cgcattcgtc ttctatatca cagcaaccac tggcacaggc acaatgccct
      241 tcggcatggc aaaactggtt ggcggcattt gcttctctct ggggctgatt ctttgtgttg
      301 tctgcggagc cgatctcttt acttccacag tgttgattgt tgttgctaag gcgagtgggc
      361 gcatcacctg gggtcagttg gctaaaaact ggctaaatgt ctattttggc aacctggtcg
      421 gcgcactgct gtttgtactt ttaatgtggc tttccggcga gtatatgacc gcaaatggtc
      481 aatggggact aaacgtccta caaaccgccg accacaaagt gcaccatact tttattgagg
      541 ccgtctgcct tggtatcctg gcaaacctga tggtatgtct ggcagtatgg atgagttatt
      601 ctggccgcag cctgatggac aaagcgttta ttatggtgct gccggtcgcg atgtttgttg
      661 ccagcggttt tgagcacagt atcgcaaaca tgtttatgat cccgatgggt attgtaatcc
      721 gcgacttcgc atctccggaa ttctggaccg ctgtcggttc tgcaccggaa aatttttctc
      781 acctgaccgt gatgaacttc atcactgata acctgattcc ggttacgatc ggtaatatta
      841 tcggcggtgg tttgttggtt gggttgacat actgggtcat ttacctgcgt gaaaacgatc
      901 accattaatg gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt
      961 acatgtccga gcttaatgaa aagttagcca cagcctggga aggttttacc aaaggtgact
     1021 ggcagaatga agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg tacgagggtg
     1081 acgagtcctt cctggctggc gctactgaag cgaccaccac cctgtgggac aaagtaatgg
     1141 aaggcgttaa actggaaaac cgcactcacg cgccagttga ctttgacacc gctgttgctt
     1201 ccaccatcac ctctcacgac gctggctaca tcaacaaagc gttggaaaaa gttgttggtc
     1261 tgcagactga agctccgctg aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg
     1321 aaggttcctg caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa atcttcactg
     1381 aataccgtaa aactcacaac cagggcgtgt tcgacgttta cactccggac atcctgcgtt
     1441 gccgtaaatc cggtgttctg accggtctgc cagatgctta tggccgtggc cgtatcatcg
     1501 gtgactaccg tcgcgttgcg ctgtacggta tcgactacct gatgaaagac aaatacgctc
     1561 agttcacctc tctgcaggct gatctggaaa acggcgtaaa cctggaacag actatccgtc
     1621 tgcgcgaaga aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa atggctgcga
     1681 aatacggcta cgacatctct ggtccggcta ccaacgctca ggaagctatc cagtggactt
     1741 acttcggcta cctggctgct gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta
     1801 cctccacctt cctggatgtg tacatcgaac gtgacctgaa agctggcaag atcaccgaac
     1861 aagaagcgca ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc
     1921 gtactccgga gtacgatgaa ctgttctctg gcgacccgat ctgggcaacc gaatctatcg
     1981 gtggtatggg cctcgacggt cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca
     2041 ccctgtacac catgggtccg tctccggaac cgaacatgac cattctgtgg tctgaaaaac
     2101 tgccgctgaa cttcaagaaa ttcgccgcta aagtgtccat cgacacctct tctctgcagt
     2161 atgagaacga tgacctgatg cgtccggact tcaacaacga tgactacgct atcgcttgct
     2221 gcgtaagccc gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg
     2281 cgaaaaccat gctgtacgca atcaacggcg gcgttgacga aaaactgaaa atgcaggttg
     2341 gtccgaagtc tgaaccgatc aaaggcgatc tcctgaacta cgatgaagtg atggagcgca
     2401 tggatcactt catggactgg ctggctaaac agtacatcac tgcactgaac atcattcact
     2461 acatgcacga caagtacagc tacgaagcct ctctgatggc gctgcacgac cgtgacgtta
     2521 tccgcaccat ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa
     2581 tcaaatatgc gaaagttaaa ccgattcgtg acgaagacgg tctggctatc gacttcgaaa
     2641 tcgaaggcga atacccgcag tttggtaaca acgatccgcg tgtagatgac ctggctgttg
     2701 acctggtaga acgtttcatg aagaaaattc agaaactgca cacctaccgt gacgctatcc
     2761 cgactcagtc tgttctgacc atcacgtcta acgttgtgta tggtaagaaa acgggtaaca
     2821 ccccagacgg tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc
     2881 gtgaccagaa aggtgctgta gcgtctctga cttccgttgc taaactgccg tttgcttacg
     2941 ctaaagatgg tatctcctac accttctcta tcgttccgaa cgcactgggt aaagacgacg
     3001 aagttcgtaa gaccaacctg gctggtctga tggatggtta cttccaccac gaagcatcca
     3061 tcgaaggtgg tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga
     3121 tggaaaatcc ggaaaaatat ccgcagctga ccatccgtgt atctggctac gcagtacgtt
     3181 tcaactcgct gactaaagaa cagcagcagg acgttattac tcgtaccttc actcaatcta
     3241 tgtaattaga tttgactgaa atcgtacagt aaaaagcgta caataaaggc tccacgaaag
     3301 tggggccttt tttagcacga gagccttttt tgtcagctat ctatacttta aggtgactgc
     3361 caaaacagac tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga tgggccacat
     3421 ctggagaaac accgcaatgt cagttattgg tcgcattcac tcctttgaat cctgtggaac
     3481 cgtagacggc ccaggtattc gctttatcac ctttttccag ggctgcctga tgcgctgcct
     3541 gtattgtcat aaccgcgaca cctgggacac gcatggcggt aaagaagtta ccgttgaaga
     3601 tttgatgaag gaagtggtga cctatcgcca ctttatgaac gcttccggcg gcggcgttac
     3661 cgcatccggc ggtgaagcaa tcctgcaagc tgagtttgtt cgtgactggt tccgcgcctg
     3721 caaaaaagaa ggcattcata cctgtctgga caccaacggt tttgttcgtc gttacgatcc
     3781 ggtgattgat gaactgctgg aagtaaccga cctggtaatg ctcgatctca aacagatgaa
     3841 cgatgagatc caccaaaatc tggttggagt ttccaatcac cgcacgctgg agttcgccaa
     3901 atatctggcg aataaaaatg tgaaggtgtg gatccgctac gttgttgtcc caggctggtc
     3961 tgacgatgac gattcagcgc atcgcctcgg tgaatttacc cgtgatatgg gtaacgttga
     4021 gaaaatcgaa cttctcccct accacgaact gggcaaacac aaatgggtgg cgatgggtga
     4081 agaatacaaa ctcgacggtg ttaaaccacc gaagaaagaa accatggaac gcgtgaaagg
     4141 cattcttgag cagtacggtc ataaggtaat gttctaattt ccccgtaaag cggcaacttt
     4201 attgagttgc cgctttttta ttccgcgctg cataaataat tcagagctct atttctaacg
     4261 cccttacagc acagcttcca aatgtattac ccaaaccgcc agtatatgtc ctgcggcttg
     4321 cgcgcatttc tcgattatgt aaaaacgggt cagatctgcc agagtcagcg tcaccgacca
     4381 caataattat ttactattcg ctttacgcgc cagcagcggg aatatcagtc ctaatcctac
     4441 cagcacaaac ggcgttgcaa cattcagcgc caactggaag gtccactctg cagtgaaggc
     4501 ttccattttc gggaagatcc ccgtcaaaca ggcaaaggcg gtaaaggcaa aacaccaaat
     4561 cccgacggtc attgccagag gcttgttatg aataaagaca tattctggtt tatatttctg
     4621 cgccaggcga acgactgcaa taaatgcaac aaatacccat aaataacgca gcggcatcac
     4681 taccgagtta agattcaaca gccatttata gagattgttc atatcaccga tgccgagagt
     4741 cggcagcata atcaggatcg ccaccagcac cagggtcaga aaatagccat taacgggcgt
     4801 accagaagcg ttggtacgac ataaactggc tggaatatat ttgctgtcag catcacctaa
     4861 tagcacttta agcggggcat caatcgagaa taccagcgca gctacttgtc ccagggtatt
     4921 cgcaatggcg taaatcacca ttaaagtatt acccatgttg taatactcgc ccagcttctg
     4981 aaaggcgtaa tattgaccgt tagtcattaa gtcatccggg atattacgcg agtcaaacat
     5041 catccccatc gccagtgagc ccagaatggc acaaaccgca accatcaccg ccaggcataa
     5101 catccctttt ggaaattctt ttcctgggtt gcgcgtttga ttaacgtaag gagaaatctt
     5161 ctctgctccg ccaaccgcga aaaccagcat tgaaatagtg gtaatgtagg taaagtcgat
     5221 atgaggaatg aacgtttccc aggtaatgtt tgtggtcgca atatgcactt cagtaatcgc
     5281 aggcgcggtt accgccatcg ccacatacag gagcgacata acaaacatcg caatccctgc
     5341 cacagaaccg acgattttca gcgatttcat accgcgtgaa gcaacccaca taaagaagat
     5401 aaacagcacc agcgttaacc cctgtaacgc tacgactgaa tattctttga ttagcgaacc
     5461 gtcgcctttc atcgcccaac cgagcgcaat cagtattgcc tggggttttt gtgccagata
     5521 gggaatatgc accacccagt aggtccacgc ggcgaggtaa gccagtccgg gtcccatcgt
     5581 gtgtttaatc caggtactga ccccgccttt cccatctttg aaggtcgagc ctaactgacc
     5641 aacaattagc gcataaggtg tgaaatagag cgcaaagata aacacccatg aaaaaacaac
     5701 caccagcccc tggttggcat agttattaac aacgttgcca aaaccccaga cagtgataaa
     5761 agacatcagc gcaatgttgt accatcgcag ctgtttttcc tgaacattcc cagccataga
     5821 caaatccttc gccaaaatta aaggggcatc tgtaaaacga gggattatgc gcggcaaata
     5881 agaagtgcgg aaatagcgaa atgcaaaatt ataacctgct gcacatttta tttttaaacg
     5941 aataaaattt ggaaaaaagc aagtcagata gaagaaacaa aaaagcagag tgtgaaacag
     6001 taagttcaca ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg
     6061 gcgttgcgaa gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat
     6121 aaattatcgg agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc
     6181 acggtatagc tcaacagtaa ggcctggttc atcgccacca gttgatgatg ttcaactttc
     6241 tcgcaagccc aggccatcgc caccggatat agcgtaaagc cagcggcacc gaggatgaat
     6301 aacgctgggg ccatcgccgc ctggctaagc atcgcgatac tgccgagaat gacgacaaaa
     6361 acctgaacac gcaacaccag cagtcgacca aacttatccg ccagacgtcc aatcggccat
     6421 tgtccgagga tacccgcact gaccagtacc gccatccaga aaccaatgct ggcattactc
     6481 acccccttgt gattgaggta caacggcatc aggccatata gagatcccag aacgattcct
     6541 gagataatgc agccattcac gccaagccgc gcctgacgga gttttagcat tgacgtaatt
     6601 gacgtcgaat catggttttc agcctgctga ttcaggacac gcgtaaacaa cagcggtaag
     6661 atccctgcca acgtcaaacc tgtaacccac ggcaatacgc tcatcagctc ggttgagact
     6721 ttgctgacca gtaactggcc taaaaacgtt cccacgtaat aaaccatcat atacgcagca
     6781 agcaaacgcc cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacgactacc
     6841 caaatcatgg cacagccgac gcccgcgaca aaacgccacg ccaaccagct ccagaaacca
     6901 atcatcaggc caaggcccgc acagccagcg gcaaaaatga aggaggccag ataatagctg
     6961 cggttaaagc caatgcgttt aatgacatac cctgtcagca atgtaccgac aaggttgccg
     7021 gtaaaatagg atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc
     7081 caaagcggca cgagtgtatt taacaccgcg atcgccagag tcaacaaaag caggccagac
     7141 agcaaaagca tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa
     7201 atttcatgcg catcatgcca ccgccaaaaa cattgtcaat gggtgaaaat acgcgcatat
     7261 cgcaacggcg gtattatcga tttatattac tcattcataa cgctaatact gaagagatga
     7321 ttaatctcat ctgattgttt ttattgatct aacacaaatt tcatacgctt cgttcagtcg
     7381 aaaaatttca tggtcacagc aaactcatat ttcccgtcta tgctttcaga gcaatgcatc
     7441 gcgtctgacc gatttcagac gtaccactga ctctcgaaag gagaagagga tgaccaaacc
     7501 gtatgttcgt cttgataaaa atgatgctgc cgttttgctt gttgatcacc aggctggttt
     7561 actttccctt gtacgggata tcgaacccga taagtttaaa aataacgtgc tggcgctggg
     7621 tgacctggct aagtatttca acttaccaac tattcttacc actagttttg aaaccggtcc
     7681 taacggccca ttagttccag aactgaaagc acaattcccc gatgcacctt acattgctcg
     7741 cccgggcaat attaacgcct gggataacga agattttgta aaagcagtca aagcgacagg
     7801 taaaaaacag ttaattattg ccggtgtggt aaccgaagtt tgcgtggcat tcccggcgct
     7861 ttcagccatt gaagaagggt ttgatgtctt cgttgtgact gacgcgtcag ggacctttaa
     7921 tgaaattacc cggcattcgg catgggatcg catgtcgcaa gcaggtgcgc aattgatgac
     7981 atggttcggc gtggcttgcg agctgcaccg tgactggcgt aatgatattg aaggactggc
     8041 gacgttgttc tctaaccata ttcctgatta tcgtaatttg atgaccagtt atgacacgtt
     8101 aacgaagcag aaataaaaga gaaccggggc cgaaaccccg gttgttactg attagcttgc
     8161 gacggccatc cccacggtca tatgcaaacc atagaatacg ccgcgaccga ttaattcccc
     8221 tgccagaagc agaatgaatg agacagatag tagcggaacc gcaggctgat aaccttttag
     8281 ctgtggtgca atccacaagc acagggcaac agccaaaagc acgatccgcc aggacatcag
     8341 cgcaccatag tccggcacca gcgctgcggc ctgctgcaca gaactatgaa tagtcgccag
     8401 ctctgcgccc tgcatcactg acatcacgcc actcactacc agtgcaagta cagaaatggc
     8461 tggcagcaga cgcatcgccc agccatcgac gccagccaga ctcagcaaca ggtaacccag
     8521 taacgggccg cccataaaca ttgtcaggaa gaagcccatc ggcgtccaga tgctgtacca
     8581 ggtcggaacg gtatcaatgc tgttatagac acgcaccatc atccagacaa agactacgcc
     8641 aagaaccatc gtcactacca gccacagcgt acgcaatgcc ggtgacagct ttttcagcat
     8701 tgccagcaac cagccgatgc cgcctacggc aaaaaagatg gaaccgctgg cgatttcgtt
     8761 actgagtgct gaagccccta cccggttgag cgagttaaag gcgcgcatcg gtgaaccaag
     8821 atggagcata gaggcgataa agccaatgcc catcagcacc cataagccaa acatgcaggc
     8881 gataacacgc tgctgggctt ctgctcgcag gtcgcctttg agcagcgcca aagccagaac
     8941 gataaaacca cccgctacac attgcccgaa gaccgtgaag atcatcagcg gccattcatg
     9001 ccatccactt cccatctcac acctccttcg ggtttgccag atagccagtg gtatccccgg
     9061 tcgggcggct attggcattg ggtttgatca caatattcgg tttggtaaag tgagctcgcg
     9121 gcaacggcgc aacggccgcc agatcgccat gttttttacg cagctcgtcg atagggccga
     9181 aatccagcgc ccgcagcgga caagattcaa cacagatcgg ctttttaccc tccgcaacac
     9241 ggtcataaca accatcgcat ttggtcatat ggcctttcgt ttcgttatat tgcggtgcgc
     9301 catacgggca agccatatgg cagtagcggc agccaatgca cacatcttca tcgaccacaa
     9361 caaaaccatc ttcacgttta tgcatcgcac cgctcgggca gactttagta caagccggat
     9421 cttcgcagtg gttgcacgaa atcgacagat agtaggcaaa cacgttctgg tgccagacac
     9481 cgttatcttc ctgccagtcg ccaccagcat attcataaat gcggcggaag ctgacttctg
     9541 gtgtcaaatc tttgtagtct ttacaagcca gctcgcaggt tttgcaaccg gtgcaacggc
     9601 tggaatcaat aaaaaatcca tactgggttg tcatcggtta ctccttacac cttttcaacc
     9661 tgaacaaggt ttgtatgtga cggattcccc ttagcgagag gagacggacg ttgagtggtc
     9721 agtacgttaa tacaaccacc cttatcgaca cgttttgcat ccgggtcata ccaggcacct
     9781 tcacccagtg cgaccacacc cggcatcatt cgtggcgtca ctttcgcctc aatatgaacc
     9841 tcaccacgat cgttaaagat cctgacttta tcgccgttgt ggataccgcg tttttgggca
     9901 tcaagcgggt tgatccacat ttcctgacgg caagccgctt tcagcacatc aacgttgccg
     9961 taagttgagt gaacgcgaga tttatagtgg aaacctgtaa gctgcagcgg atactgtttg
    10021 ttcagcggat cctgataact ttcaaagccc ggcgtgtaga tcggcagtgg atcgatcaca
    10081 tcgccttctg gcaattccca ggtagcggca atgtcagcca gcgcctgcga ataaatctca
    10141 attttacccg atggcgtggt cagtggattt gcctgcggat cttcacggaa ggctttataa
    10201 gcaacgtgat gcccttgtgg gtcgcgcttt ttaaagatcc cctgcttgcg gaactcttca
    10261 aacgttggca gttcaggaat cgcttcccgc gactgggcat acagatgccg catccactct
    10321 tcctgggtac ggccttcagt aaactgttgc tcaacgccaa gacgttttgc cagttcgctg
    10381 gtcatttcat agatggtctt acattcaaag cgcggtttaa tcacctgatc gttgaaaatc
    10441 acgtaagaca tattcccgca ggatgcatcc agtgcaaagt ccatctgttc ggaagcggta
    10501 cagtcaggca gcaggatgtc agcatatttc gccgatgaag tcatgtggca gtcgataacc
    10561 acaatcagct cgcacttctt atcatcctga aggatttcat gggtacggtt gatttcagaa
    10621 tgctggttaa tcaggcagtt accggcatag ttccagatca ttttgatcgg cacatccagc
    10681 ttatctttcc cgcgcacacc atcacgcagc gccgtcattt ccgggccacg ttcaatggca
    10741 tcggtccaca taaacatcga aatgctggtc tggatcgggt tttccaaggt cggcatacgg
    10801 acaaacggta agctgtatga accttcacgc gcgccgctgt tccctccatt aataccaacg
    10861 ttaccggtca gaatcgccag catcgagata gcacgggttg cgatttcacc gttagcgtga
    10921 cgctgcgggc cccatccctg gctgataaac gccggtttgg tactgccgat ttcacgagcc
    10981 agtttgatga ttttgtctgc cggaacaccg gtgatttgcg aggcccattc cggtgtttta
    11041 gccacgccat ctggcccttc acccagaata taagctttat agtggccatt tttcggcgca
    11101 ctggctggca gggttttctc gtcatagcca acgcaatatt tatcgaggaa tgcctgatcc
    11161 accaggtttt cggtgatcat gacgtacgcc agaccgttaa ccagtgcggc atctgttcct
    11221 ggacgaatag ggatccactc atcttcgcgc ccggcaccgg tgtcggtata gcgcggatcg
    11281 atgatgatca tgcgggcatt agatttctgg cgtgcctgtt caagatagta agtcacccca
    11341 ccgccactca ttcgcgtttc gccagggtta ttaccaaaca gcactaccag cttactgttt
    11401 tcgatatccg acgggctgtt gccatctgcc cagccgccgt aggtatagtt cagaccttcc
    11461 gcaatttgcg cggaggagta gtcgccgtaa tggttcagat agccgccgca gcagttcatc
    11521 agccgcgcga ccagggtatt tcccggcggc caggagcggg tcatggtgcc acccagcgta
    11581 ccggtgccat agttaagata gatggattcg ttgccgtact ctttgatcag gcgctgcata
    11641 ttggtcgcga tgatgtcgta ggcttcttcc cagctaatgc gctcgaattt gccttcaccg
    11701 cgcgccccga ctcgtttcat cggatatttc aggcggtccg gattatagac acggcgacgc
    11761 atggaacgcc cacgcaaaca ggcgcgaacc tggtgcaggc cgtcgtaatt gtcatcgccg
    11821 gtattgtccg tttcgacata tttgatttca ccgtccacga cgtgcatacg tagcgggcag
    11881 cgactaccac agttaactgt acaggcgctc cagataacct tttcgtctga ttttgttgga
    11941 atggcgctat cgacagcgtg cgcaatccga ctaaaaggta atgttaatgc gctgctggcc
    12001 attgccaggc cgccgatcgc tgtcgttttt accaaaccac ggcgactcac ctcagcagcc
    12061 aataccgcat cagggatttt cgttttcata atggctcact caagcttgct cacgattgat
    12121 tccagtaaca aaccgtattg tgtaattatt tctatagcag atcgctatat aggcttgtat
    12181 acatcgaata atttgaaaat caaagagata ggtagatttt cactcgaaag tagtattacg
    12241 tgtaagtgag gagtaattat tgttcggtat caaagggaga caaaaaaagc gcccgcagga
    12301 ggtttaacgc tacttactcc ccttgatgat ttgcttattt ttcaatgagt aaatcaaaac
    12361 aaggggagca aataagcaca agttacaact aatcgatgga cagtttatgg acactttcag
    12421 ccagcggatt aaagctgatt gcatcctgta aaaagtccgg cgaaaaatgc gcatacgtca
    12481 tcgtttgttg aatcgtggca tggcccagga ttcgttgtag cgtaacgata tttccgccat
    12541 tcatcatgaa atgtgtagcg aatgtatgac gcatgacatg ggtggcctgc cctttcggta
    12601 agtccggctt cacctcctga aggatctccc gaaatctgag gtaatcagcc tggtacaaca
    12661 atccggttct gcgggtttta attaacgcaa gtaccgcatc agaaacggga acggcgcgag
    12721 attttccgtt cttggtgtta aagaacatca cgcggttacc tacaatatgt tccccacgta
    12781 aaccagctgc ttcaccccac cttgcccccg tggaaagaca cagaatggca acacgccgcg
    12841 catctccatc gagtcgttca agcagtcgtt caatctcctc atctgaaaga aaagccatct
    12901 ctgtgttcct gactttcagc ttacgtattc cccgaaccgg gttttcgtta tggaacacct
    12961 caccctcaat cagcgagctg aacattgcag acaaggcaca caaatcgcgg ttaatgctgg
    13021 aaggcattaa cccggcctgt aatttgtcgg accggtaccc gagcataaat tttcgcgtca
    13081 tctgactggc acgaggatca cccatctcac ggtttatctt ttccagtctg accctgtagg
    13141 tatccccgta atcctggtta cgaccatcaa gcagccacca ggcctctatc agttcggata
    13201 accgacgctg atctgctggc ttagcctgcc atggcttatc atgaaaattc tgcaaaacat
    13261 acctttcaaa ctcctgcgcc tttgacttca gggtaaaaat cttacgaatc cgctttcctt
    13321 ccgtcccttg tggccttaca tcaaccttat aacgtccgtc tttgagttgc ttaatcgaca
    13381 tgattagccc ctccaacgga taaattaacc aaacactccc tgcgggatga aacccgctcg
    13441 atgtgtaacg tcagccagca ttgcggcctg atcgggatga tttttgggta acgtcgattg
    13501 aagccagatc gccctccgac tgagaagatc catcaagaga gagagccggg gcaatttgcc
    13561 cggcggcagg tagcgtctta tcctttaaca gccaaaacat atacttttca aatcgatctg
    13621 cctgaagaac cgactccacg attttagctc tggcgctctt ttgccctgat tcataattcc
    13681 ttacggtggc cagagataac cctgtaattt cagcaaattg tttttgcgtt aagccctccg
    13741 ccttacgtat ctggcgtaac ttttccgcgt agtctattga caagataacc atttgagtat
    13801 taacctctcg aaaaagataa cctttcgatt atcttcacaa cccaaaacaa accttcataa
    13861 ggtgcaatag gcgcacctgt gcgagaggat aacaaatgcg ggacttcatt gccgataaag
    13921 agttatctga caccgatcac acaactggag cggcgtacat caacgccaat accagtcaac
    13981 aagaagcaac agataacaac gacgaacaaa agccacagcg cacatataaa aaaggggcaa
    14041 ccctgcggct ggatggccca atagccgggc tttgctcact tgaaaaaggg gccgcctaca
    14101 tcggattaac aaaaacagca ttacgcgcag ctcttcagcg taaccagatg ccaggacata
    14161 aaacccgctc aaaccctgaa gatgagaatt cggatggtgt ttggtggttt aacgtcagag
    14221 agtgggacaa gctggccgac gaactcccgg aatgcgagcc accagaatgg cacaactgga
    14281 aaagctactg gacatacgac cgccagaaaa gaaagtttac cccggctgat aaggaagatt
    14341 gccagaccgt caatggaaaa cgggtttaca aaggcagaaa gtcaaagctg gaacagagcc
    14401 aggatgtaaa cgaacggtga tgatcgatga gcggcaaaaa taaatcagaa aaaaccgcaa
    14461 ccgtccttaa gcagttaagc ctcctgaaag aggaacacaa ggacgccacg atactgatgc
    14521 atctggaagg ctcaccgaaa ataacaatca caacaaccta tagcgagtta cacaaagagc
    14581 ttgaaggatt aattaacttt tgttcaaagc taaaagcgaa tgaaaacatt gatgacctga
    14641 tacttaaacc agaagcagag ttcggacact tttgcgaaag tagagaaaat ggatgcatcg
    14701 gaataatgct atacgcaagc cgtggaataa tgattaatct gcccgttgag caggctgtgg
    14761 atatattcgg agaaatggca cttaataaaa ccactattta caaaagaaaa atgccggagt
    14821 aaccaggcat ggttattagt atgttggtcc cttagaaaag ttccaggaac tcttgcgagg
    14881 gtatttatga agtacttttt cgcggacatc gttagccaga tctctgattt gatcgtgctt
    14941 atcatcagaa gacggaggaa cttcggggat tttatccacg atttcaatta ctgatttaag
    15001 catttttatt aattcatcgt cgtaagacat tgttatctcc tttttctggt tgtgtggaaa
    15061 caccagaata ccacgcgccg ggcgtggtta aacatcccgg cactgatttt catttaactt
    15121 aaacggggtt tttcgatatg acacagagac gcagcttccg cactggcaca gaacgccacg
    15181 cagcccgttt ctcaaccagc gcatcacgca gcaactcgca ctacagcctg caggaaacgc
    15241 acgccacacc tgacgggcat ctggttaaac aaaccggtaa acacacctgg ctgattaatg
    15301 atgccggaat tgtcatacac agaggcgcac gcaacccgat taccggaaat acctgttact
    15361 ccctgactcg cggagatgag cagttcgggc aggatttcac cctacacgaa gcattaagaa
    15421 cggcagaccg attaatcagc ggtcgcagtt ttcagcaccc ggaacatcgc cgctgataaa
    15481 taaaaggatt cagccatgat cgaagcctac aaaatactta ttaataatgc gttgcagcgt
    15541 tatcacttcg aactcgcaaa ccggggtgta acccgggacg aaggacttcg taactcactg
    15601 cggggcgcaa ttatgggact gtataactgc gcactgggca gcgatgaccg cgaagcactg
    15661 gaagaattaa aagttattgt ggccgaactg tttgaaggtg aaatcgttga gccttatttc
    15721 acagaggtgg cagcatgagc gtatttcttt catgggtgat tctgattatt tccgtgctta
    15781 tggcgattgg cattatgcga attatcgaag cgctgaaaaa tatcgaatct ttatttactg
    15841 gcgaagaacg ccgtaaataa ataaccaacc ggacagaaaa cattaaaacc atccgcatct
    15901 gcggagggct tcactcaatc cagaaacgga gaaacagcta tgagcatcaa tacacctaca
    15961 gaaataaaca aactatttac cgacccggcg catattgaat ttacgcaaaa aacgctgact
    16021 gaaataacca atgtactgga tgaaagaata tcagaaatag ataaagacaa gcattttgcc
    16081 acctacatgg ccctgcaaat gcaaagcatg gcaatggtga cagcaaaaca gaccatcagt
    16141 gaactctata tgaaaaacct gcgcctcgag cgcgaactgg cagaactccg gacacagtcc
    16201 ggccagtttt cagcgtgagg ggcaggtcga tgaacagcat atttaccgcc accatgctga
    16261 cgcgtttcac cgatgccgtg ggccacgagt tcatggtgga aagtcatttg atcacaacga
    16321 ccacgccgtg cccgtcagac gctgattatc tctacatcca tctggctgac ggaacacaaa
    16381 tcaccgcgat tgccagtacc gttcgcgaag taacggccat cagaggggca tggaaatcag
    16441 aaacacaagc ccacggagaa ctgcggccat gatcagcaag tacaccacca acgcacgtaa
    16501 acccggtaaa gacgcacgcc accacatcgc cgtactccgc aatctgttac tggaggaaat
    16561 cgacgacttc acggcaacgc tgagcggtcc gggcgcacca gtaacaccag gtgagatcca
    16621 ccgggacctt aaacgccgta ttgaaaacgc cattgattac accctgaacc cgactgaata
    16681 accggaactg caaaggagat ctcacgatga ccattctgga ctacatcgca gccaatccgg
    16741 ggtgcagcgg aagtgaaatc gccgcagcac tgaatacccc aaccacagcc attaatgctg
    16801 agttacgccg actctggcgc ggcggcttag tcatcagaac aaaccgcagc acaggtggtc
    16861 gcgctcgcaa aacaggaggc caggcttctt accacgtaaa cccgatgccg ttcgggtgta
    16921 gcaatccact tgctcacatg tttaaccagc tactgaagga agccagagca tgagcaccat
    16981 caaccaccag aagctacgcg aactggcatt tgccctgaaa cgaatggcaa cgcctcaaaa
    17041 attactggca tttcgcgcaa tgctctcgcc gtctgctgtg ctggcactgc tggatgagct
    17101 ggagcacgcc agaaccacgg ctcctgccat tcgcctgacg ctccatcatg aaatcgctga
    17161 tttctgcgcg acgttggagt cgccgggcga accggaaacg ccggaagcaa tacagcaaga
    17221 gctgctgcaa cgcattgaca aggtttttga tttttttctg aaccagtaag aaaccagaac
    17281 atgcacacac aaaaaaaccg cttgccatgc cgcaatcagt caggttacat tcccgctgca
    17341 cctcataaaa cgggtgccgg gattaccacc ccgccgacta gcaaagcgca cagccgcgcc
    17401 agcggttttt ttgtgcgtac tgtatcgcca cgtctttttc gcacacgaat tatggcgggg
    17461 cgtacggggc cgacttcggt cgggccggat tctttgctag ccggttgtgg taaccctgta
    17521 cgtctcgcca ccctgagatt accgcctcag gttggtgagt tttcagaact tactagcaaa
    17581 gaggccacac catggcaaac cgcaagcaac agcgcgcccg cgctgagcgt cttcacaccc
    17641 ggactgaaat caaccgcaga ctctggcgcg caacacgcgt cgcgcaaatc atgcacatca
    17701 acatgctgca tgagcgcacg cacgcgctat caaaccgtta ctgcgctgct gtcttcagct
    17761 acctggcgga tgacctggca gagctgcagg accttatcaa ccagcaacac caccactaaa
    17821 caacatcctg aagccatccc gcacccgtgc gcgggaggct tccgcacgtc tgtgaccgga
    17881 ggttctcatg aagaacgtta agggactttc agatattcag gattttttta actggatgtg
    17941 ggcagtggag ccccggctgg cttcccgcct caacgactgg cacgactatt accgccgcgc
    18001 ctgtaaacag gccgccagac gcgtcaccac atacggggat ttctccttca ccgttgacga
    18061 ccgttaccgc gtggacgtga aaggtgatgg ttttggttta tttcacatca acggaaccga
    18121 cagcaccgcc atcagctggt cgtcgtcaga gatgacgctt gttctggaaa tgacaaacca
    18181 cagcgtggcc gtgaaaggca tatacaccgc agaagacttt atcggtgaat accagcgcct
    18241 tctcgtgctg taccgcgcgg cgtttcaggc ggcgactgcc ggggagtgcg acgcatgagc
    18301 gaccataacg cacaactggc gtacccgtgg aacgccccgc taccggttat cgatcctgaa
    18361 acgttcggca gagcgcagcc aaccgcatta cgccaggcca ttcagagcta catcaacgag
    18421 gatattcgca ctgacgccag actggatgaa gaaaccgtcg ccttcctgac aaccacagac
    18481 gaagggaagc gaattaattc gcatctttac cacgacgaag agcgtcggat caggctggaa
    18541 aaactggcgc ggcacaaaca cgagaatccg ccaacactgg tatctgaagc catggcagag
    18601 ctgcgcgccc tgccgccgtt tcttgccggt ccgctcatcc gtgacctggc gcgcctgagc
    18661 cgcaaacagg agagcgcacg ccgggaaggg cttaagaata acagcgcata tgcggcggat
    18721 aacttcgttc gtcacgggct gcgtgcccgc ctgaaacgta tcgcccgtgt gaatgaacgc
    18781 tttgccaccc cggcctttaa ggccaccgcc gcgcgcgaac ggctggatga actgcttatg
    18841 ctgccacagc tgagccgtga cgaaatccag cgccttgcca ccctgaccgc caccgcgttt
    18901 tccaccgcac tggagcgcgc atgtgatga
//
LOCUS       DAZGBC010000092        18816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGBC010000092 DAZGBC010000000
VERSION     DAZGBC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.092"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..879
                     /gene="ydiY"
                     /locus_tag="ACHK1J_003103"
     CDS             121..879
                     /gene="ydiY"
                     /locus_tag="ACHK1J_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO7912342.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(932..1039)
                     /gene="yniD"
                     /locus_tag="ACHK1J_003104"
     CDS             complement(932..1039)
                     /gene="yniD"
                     /locus_tag="ACHK1J_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO7912343.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1214..3112)
                     /gene="espL1"
                     /locus_tag="ACHK1J_003105"
     CDS             complement(1214..3112)
                     /gene="espL1"
                     /locus_tag="ACHK1J_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO7912344.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDTPTPSSEPINHFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLMQTKSANETPG
                     LYISMLYGFDEIIDIFLNTLTAPIAQKHLNKKMVMDILAMKTRDGEPGLFAAMENNHP
                     LCVTRFLSKIYGIAVKYNLSKINIIDLLKGATVHGTPALYIAMSKGNKDVMLSYISTL
                     DTFAKKYSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFS
                     ADELKTYL"
     gene            3635..5563
                     /gene="thrS"
                     /locus_tag="ACHK1J_003106"
     CDS             3635..5563
                     /gene="thrS"
                     /locus_tag="ACHK1J_003106"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO7912345.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            5567..6109
                     /gene="infC"
                     /locus_tag="ACHK1J_003107"
     CDS             5567..6109
                     /gene="infC"
                     /locus_tag="ACHK1J_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO7912346.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            6206..6403
                     /gene="rpmI"
                     /locus_tag="ACHK1J_003108"
     CDS             6206..6403
                     /gene="rpmI"
                     /locus_tag="ACHK1J_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO7912347.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            6456..6812
                     /gene="rplT"
                     /locus_tag="ACHK1J_003109"
     CDS             6456..6812
                     /gene="rplT"
                     /locus_tag="ACHK1J_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO7912348.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6929..7053
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6935..6979
                     /gene="pheM"
                     /locus_tag="ACHK1J_003110"
     CDS             6935..6979
                     /gene="pheM"
                     /locus_tag="ACHK1J_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO7912349.1"
                     /translation="MNAAIFRFFFYFST"
     gene            7262..8245
                     /gene="pheS"
                     /locus_tag="ACHK1J_003111"
     CDS             7262..8245
                     /gene="pheS"
                     /locus_tag="ACHK1J_003111"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO7912350.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            8260..10647
                     /gene="pheT"
                     /locus_tag="ACHK1J_003112"
     CDS             8260..10647
                     /gene="pheT"
                     /locus_tag="ACHK1J_003112"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO7912351.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            10652..10951
                     /gene="ihfA"
                     /locus_tag="ACHK1J_003113"
     CDS             10652..10951
                     /gene="ihfA"
                     /locus_tag="ACHK1J_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO7912352.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            11052..12032
                     /gene="btuC"
                     /locus_tag="ACHK1J_003114"
     CDS             11052..12032
                     /gene="btuC"
                     /locus_tag="ACHK1J_003114"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO7912353.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            12095..12646
                     /gene="btuE"
                     /locus_tag="ACHK1J_003115"
     CDS             12095..12646
                     /gene="btuE"
                     /locus_tag="ACHK1J_003115"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO7912354.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            12646..13395
                     /gene="btuD"
                     /locus_tag="ACHK1J_003116"
     CDS             12646..13395
                     /gene="btuD"
                     /locus_tag="ACHK1J_003116"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO7912355.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEIRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            13474..13938
                     /gene="nlpC"
                     /locus_tag="ACHK1J_003117"
     CDS             13474..13938
                     /gene="nlpC"
                     /locus_tag="ACHK1J_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO7912356.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            14185..14898
                     /gene="ydiV"
                     /locus_tag="ACHK1J_003118"
     CDS             14185..14898
                     /gene="ydiV"
                     /locus_tag="ACHK1J_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO7912357.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVARLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIKAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            14961..16397
                     /gene="selO"
                     /locus_tag="ACHK1J_003119"
     CDS             14961..16397
                     /gene="selO"
                     /locus_tag="ACHK1J_003119"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO7912358.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSNSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(16401..16592)
                     /gene="hemP"
                     /locus_tag="ACHK1J_003120"
     CDS             complement(16401..16592)
                     /gene="hemP"
                     /locus_tag="ACHK1J_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO7912359.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(16724..17770)
                     /gene="aroH"
                     /locus_tag="ACHK1J_003121"
     CDS             complement(16724..17770)
                     /gene="aroH"
                     /locus_tag="ACHK1J_003121"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO7912360.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(17927..18760)
                     /gene="ppsR"
                     /locus_tag="ACHK1J_003122"
     CDS             complement(17927..18760)
                     /gene="ppsR"
                     /locus_tag="ACHK1J_003122"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO7912361.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         4773 a         4311 c         4887 g         4845 t
ORIGIN      
        1 tgctaagtta ttcgcgcctt tttataattc gttacctttc cagggacgct aatctttgat
       61 ccttaagaaa gtttccggga ccgcgatggt ctcttttttt gttgtacgga ctcttgataa
      121 atgaagcttt tgaagacagt tcctgccatc gttatgttgg cggggggcat gtttgcgtca
      181 ctgaatgcag ctgccgatga ttccgttttt actgtcatgg acgatcctgc cagcgctaaa
      241 aaacctttcg aaggtaatct gaacgcagga tatcttgcac aatcaggcaa tacgaaaagc
      301 tcctcactta cggctgacac caccatgacc tggtatggac aaaccactgc ctggtcgctg
      361 tgggggaacg ccagtaatac ttcttctaat gatgagcgtt cttcagaaaa atatgcggcg
      421 ggcgggcgta gccgttttaa tttaactgat tatgactatt tatttggtca ggcaagctgg
      481 ctgacagacc gttataacgg ctatcgagag cgcgatgtgt tgaccgcggg ttatggtcgt
      541 cagtttttga atggcccggt acacagtttc cgctttgaat tcggtccagg tgtgcgttac
      601 gacaaatata ccgacaatgc cagtgaaact cagccgctgg gctatgcctc cggggcttat
      661 gcatggcagt tgactgacaa cgctaaattt acgcagggtg tttcggtctt tggcgcggaa
      721 gacacaacgc tgaactccga aagtgcatta aatgttgcta ttaatgaaca ttttggactg
      781 aaggtggctt acaacgtcac atggaactct gaacctccag agtccgcacc ggaacatacc
      841 gatcgtcgga caaccttatc gctgggctac agcatgtaaa attatctgcc ggatcgtgtt
      901 atgcggtctg gctgctttcg tttgtcactg ctcagcccca aatcattgct gcccaacgta
      961 gcaacaacat agcgccacag attgccagta gcaccaccac catcttccag tgttttttat
     1021 caaagcgttt agtcggcata ccagtgtcct gcatatctct tgtgcgtgat aacggagtgt
     1081 aacaaaaggg acaggcatca agggattttt tgttattaca gccgtcgggt ggcgttgtac
     1141 acctgatcac tgtttctgcg ttttatgaaa aaataaaatt atgtatcgat atataaatat
     1201 cgtaaatagc tggttataaa tacgtcttta attcatcagc actaaaactc atgctttggc
     1261 tgattacatt tatagcagca taatatgttt ctacagtttt ataatgatta tgatgaatgg
     1321 ctatatgaac agctgacatg ttgtcatgat ttttagcggc caacagtgta aataactgat
     1381 gttggctaaa agaatatttt tttgcaaaag tatccagcgt cgatatataa gataacatga
     1441 cgtctttatt acccttgctc atagcgatgt ataaagcagg ggttccatgt actgttgcgc
     1501 cttttaataa atctatgatg ttaattttgc tgaggttata tttaacagcg attccataaa
     1561 ttttagagag gaaccgtgtg acacacaacg ggtgattatt ttccattgca gcaaataacc
     1621 ctggttcacc atcacgtgtt ttcatggcta aaatatccat caccattttt ttgtttaaat
     1681 gcttttgtgc tattggagcg gttaatgtat tcagaaagat atcgattatc tcatcgaatc
     1741 catacaacat ggagatataa agtccaggtg tttcgttagc acttttagtt tgcattaatt
     1801 taaccagatt atcttcatga atgatgtgat tatctaccag tgactgaatt tcttgcataa
     1861 atattttcac gttgtcagta tgtccgttca tcatagccaa aaataaagca ggtgtaccaa
     1921 cgctattctg cgcagttatt atttcaatga tttctttttc agggagtatg ggcaactgtc
     1981 tgaataatgt aataagctgt tgagtctggc ctgtggccat tattaacaac attgccgatg
     2041 gatgtatagg ggcaaatggc attcgtgtcg cgatacccgt gaaaatagcc cctggaactc
     2101 cctcaggtaa taggggcaga atagcaattg cacagttgcg aacataccta tcagcccagg
     2161 tctgagcatc gggacctgaa ctgatgaact tatcaagcga gagaaagccc cgtgctgtcc
     2221 tgtgggtgcc tctgacggca acgttggtat cgttcgggtc gtaaacggac acaacataat
     2281 ttaattgccc atacgccgac tgttttattc gtagccttac tgacaaagta tgattgtcta
     2341 tatataaaat cgcagacata tgactgacat tgtttgccat cattcgacag aagaaaatat
     2401 ctaaagcgaa gccaaaatcc ttactcgcga taacgcgact ccctgcagga ggatggcgaa
     2461 gtaatgctct gaactccgcg tcccggacta aggggacatg ctgctgtatg gattcaacgg
     2521 aggagtaatg tttaatcagg ttgaccttgc cttttccatt tctcaatgaa tcaagtgcgt
     2581 attgcaatga cagatggcga caaaaaatag cgtcagaagg agattgcgaa aaaaatgcac
     2641 taccattgag atttactgtt ggttgtatag ttttttctct atggattttt ttagacagtt
     2701 tcatacaatg gttacgattt tcggcagtta actgttcctg ttcaatataa tccagtatgc
     2761 ttcgcaaatg gttagcttta ataggcgtgg tttccagtaa taaggaaatg atttcttttt
     2821 tatagtgtaa tgtaataagt atattttctg catggatggg tgaagtggca aagttaatgg
     2881 taaaaagggc agtggaataa caattttgtg atatttttcg acagtttgcg gtaaattcct
     2941 gagtaacaca gtatgacagc atcgaactta agtcagaggt atcaagcagg gtgatgagct
     3001 ggcgcgcaaa atgattaata ggctctgatg aaggcgttgg tgtgtcgcta tcgatgtgca
     3061 ttctatgagg agacttataa tgagttctga taataatgtc gttttgactc ataatctttt
     3121 cgccttatat atgtctggag gttttgagga tgatgattaa ttattatcgc cagaacgaaa
     3181 tgtgcacgaa aaattgctat aagaattgtg tcacttttat cattgtgata tgaccacttc
     3241 gtcattaatc tcatgatcgt ttaaatacta ccgccaaatc atgcaacgaa ctttgctgga
     3301 tttagtttat gctgctgtaa atccgctcgc agtaaacctt tcagacgctc cgtgatgtta
     3361 tcagttgttc tttaagcgtt ttgctggtgt actcactaca aacgaattgc gaatcgaatc
     3421 aatgtgaaac ggaaaggtac aatctccctt tttttagtgt taacatcgct caaccgggtg
     3481 gaaggtgaga ggttaatgtc tttcatttgt gtacctgatt gcgaaccaat ttagcatttg
     3541 ttggctagat ggtttcgcaa tgaactgtta ataaacaaat ttttctttgt atgtgatctt
     3601 tcgtgtgggt caccactgca aataaggata taaaatgcct gttataactc ttcctgatgg
     3661 cagccaacgc cattacgacc acgctgtaag ccccatggat gttgcgctgg acattggtcc
     3721 aggtctggcg aaagcctgta tcgcagggcg cgttaatggc gaactggttg atgcttgcga
     3781 tctgattgaa aacgacgcac aactgtcgat catcaccgcc aaagacgaag aaggtctgga
     3841 gatcattcgt cactcctgtg cgcacctgtt agggcacgcg attaaacaac tttggccgca
     3901 taccaaaatg gcaatcggcc cggttattga caacggtttt tattacgacg ttgatcttga
     3961 ccgcacgtta acccaggaag atgtcgaagc actcgagaag cggatgcatg agcttgctga
     4021 gaaaaactac gacgtcatta agaagaaagt cagctggcac gaagcgcgtg aaactttcgc
     4081 caaccgtggg gagagctaca aagtctccat tcttgacgaa aacatcgccc atgatgacaa
     4141 gccaggtctg tacttccatg aagaatatgt cgatatgtgc cgcggcccgc acgtaccgaa
     4201 catgcgtttc tgccatcatt tcaaactaat gaaaacggca ggggcttact ggcgtggcga
     4261 cagcaacaac aaaatgttgc aacgtattta cggtacggcg tgggcagaca aaaaagcgct
     4321 taacgcttac ctgcagcgcc tggaagaagc cgcgaaacgc gaccaccgta aaatcggtaa
     4381 acagctcgac ctgtaccata tgcaggaaga agcgccgggt atggtattct ggcacaacga
     4441 cggctggacc atcttccgtg aactggaagt gtttgttcgt tctaaactga aagagtacca
     4501 gtatcaggaa gttaaaggtc cgttcatgat ggaccgtgtc ctgtgggaaa aaaccggtca
     4561 ctgggacaac tacaaagatg caatgttcac cacgtcttct gagaaccgtg aatactgcat
     4621 taagccgatg aactgcccgg gtcacgtaca aatttttaac caggggctga agtcttatcg
     4681 cgatctgccg ctgcgtatgg ccgagtttgg tagctgccac cgtaacgagc cgtcaggttc
     4741 gctgcatggc ctgatgcgcg tgcgtggatt tacccaggat gacgcgcata tcttctgtac
     4801 tgaagaacaa attcgcgatg aagttaacgg atgtatccgt ttagtctatg atatgtacag
     4861 cacttttggc ttcgagaaga tcgtcgtcaa actctccact cgtcctgaaa aacgtattgg
     4921 cagcgacgaa atgtgggatc gtgctgaggc ggacctggcg gttgcgctgg aagaaaacaa
     4981 catcccgttt gaatatcaac tgggtgaagg cgctttctac ggtccgaaaa ttgaatttac
     5041 cctgtatgac tgcctcgatc gtgcatggca gtgcggtaca gtacagctgg acttctcctt
     5101 gccgtctcgt ctgagcgctt cttatgtagg cgaagacaat gaacgtaaag taccggtaat
     5161 gattcaccgc gcaattctgg ggtcgatgga acgtttcatc ggtatcctga ccgaagagtt
     5221 cgctggtttc ttcccgacct ggcttgcgcc ggttcaggtt gttatcatga atattaccga
     5281 ttcacagtct gaatacgtta acgaattgac gcaaaaacta tcaaatgcgg gcattcgtgt
     5341 taaagcagac ttgagaaatg agaagattgg ctttaaaatc cgcgagcaca ctttgcgtcg
     5401 cgtcccatat atgctggtct gtggtgataa agaggtggaa tcaggcaaag ttgccgttcg
     5461 cacccgccgt ggtaaagacc tgggaagcat ggacgtaaat gaagtgatcg agaagctgca
     5521 acaagagatt cgcagccgca gtcttaaaca attggaggaa taaggtatta aaggcggaaa
     5581 acgagttcaa acggcgcgcc ctaaccgtat caatggcgaa attcgcgccc aggaagttcg
     5641 cttaacaggt ctggaaggcg agcagcttgg tattgtgagt ctgagagaag ctctggagaa
     5701 agcagaagaa gccggagtag acttagtcga gatcagccct aacgccgagc cgccggtttg
     5761 tcgtataatg gattacggca aattcctcta tgaaaagagc aagtcttcta aggaacagaa
     5821 gaaaaagcaa aaagttatcc aggttaagga aattaaattc cgtcctggta cagatgaagg
     5881 cgactatcag gtaaaactcc gcagcctgat tcgctttctc gaagagggtg ataaagccaa
     5941 aatcacgctg cgtttccgcg gtcgtgagat ggcgcaccag caaatcggta tggaagtgct
     6001 taaccgcgtg aaagacgatt tgcaagaact ggcagtggtc gaatccttcc caacgaagat
     6061 cgaaggccgc cagatgatca tggtgctcgc tcctaagaag aaacagtaag gccttcaagt
     6121 agcactgtct gtggagcctt cgggcttcac agactttgtt cgcctttgtt tcgtttatta
     6181 acaatgcgaa gtggaagtta ttaaaatgcc aaaaattaag accgtacgcg gtgctgctaa
     6241 gcgcttcaaa aaaaccggta aaggtggttt taagcacaag cacgctaacc tgcgtcacat
     6301 tctgaccaaa aaagcgacca aacgtaaacg tcacctgcgt ccgaaagcca tggtttccaa
     6361 aggcgatctg ggcctggtaa tcgcgtgcct gccgtacgca taagccgtta acgtttttaa
     6421 ctttttaatt agaatataga tacaggagag cacatatggc tcgcgtaaaa cgtggtgtta
     6481 ttgcacgcgc acgtcacaag aaaattttga aacaagctaa aggctactac ggtgcgcgtt
     6541 ctcgcgtata ccgcgttgcc ttccaggctg ttatcaaagc tggtcagtat gcttaccgtg
     6601 accgtcgtca acgtaagcgt cagttccgtc aactgtggat tgcgcgtatc aacgcagcag
     6661 cacgtcagaa cggtatttct tacagcaaat tcatcaatgg cctgaaaaaa gcctctgttg
     6721 aaatcgaccg taagatcctg gctgatatcg cagtattcga caaagtagcg ttcaccgctc
     6781 tggttgaaaa agcgaaagca gctctggcat aagccagttg aaagagggag ctagtctccc
     6841 tcttttcgtt tcaacgccat caaaacattg acttttatcg ccgtagcctt ttcaataaag
     6901 gtcttttgaa gagtaccaaa aggtaacgca agcaatgaat gctgctattt tccgcttctt
     6961 tttttacttt agcacctgaa tccaggaggc tagcgcgtga gaagagaaac ggaaaacagc
     7021 gcctgaaagc ctcccagtgg aggctttttt tgtatgcgcg ttttgaaaaa taaaaagcca
     7081 gtctatcagg cgtaattgtc gcagccagtc tggatacggc cagtgcggag aaaccggagc
     7141 gtacttaagt acgtgaggat ttcgagcact gcccgggacc aaaatggcaa gtaaaatagc
     7201 ctgatgggat aggctctaag tccaacgaac cagtgtcacc actgacacaa tgaggaaaac
     7261 catgtcacat ctcgcagaac tggttgccag tgcgaaggcg gccattagcc aggcgtcaga
     7321 tgttgccgcg ttagacaatg tgcgcgtcga atatttgggt aaaaaagggc acttaaccct
     7381 tcagatgacg accctgcgtg agctgccgcc agaagagcgt ccggcagctg gtgcggttat
     7441 caacgaagcg aaagagcagg ttcagcaggc gctgaatgcg cgtaaagcgg aactggaaag
     7501 cgctgcactg aatgcgcgtc tggcggcgga aacgattgat gtctctctgc caggtcgtcg
     7561 cattgaaaac ggcggtctgc atccggttac ccgtaccatc gaccgtatcg aaagtttctt
     7621 cggtgagctt ggctttaccg tggcaaccgg gccggaaatc gaagacgatt atcataactt
     7681 cgatgctctg aacattcctg gtcaccaccc ggcgcgcgct gaccacgaca ctttctggtt
     7741 tgacgctacc cgcctgctgc gtacccagac ctctggcgta cagatccgca ccatgaaagc
     7801 ccagcagcca ccgattcgta tcatcgcgcc tggccgtgtt tatcgtaacg actacgacca
     7861 gactcacacg ccgatgttcc atcagatgga aggtctgatt gttgatacca acatcagctt
     7921 taccaacctg aaaggcacgc tgcacgactt cctgcgtaac ttctttgagg aagatttgca
     7981 gattcgcttc cgtccttcct acttcccgtt taccgaacct tctgcagaag tggatgtcat
     8041 gggtaaaaac ggtaaatggc tggaagtact gggctgcggg atggtgcatc cgaacgtgct
     8101 gcgtaacgtt ggcatcgacc cggaagttta ctctggtttc gccttcggga tggggatgga
     8161 gcgtctgact atgttgcgtt acggcgtcac cgacctgcgt tcattcttcg aaaacgatct
     8221 gcgtttcctc aaacagttta aataaggcag gaatagatta tgaaattcag tgaactgtgg
     8281 ttacgcgaat gggtgaaccc ggcgattgat agcgatgcgc tggcgaatca aatcactatg
     8341 gcgggcctgg aagttgacgg tgtagaaccg gttgccggta gcttccacgg cgtggtcgtt
     8401 ggtgaagtgg ttgagtgtgc gcagcatccg aacgctgaca aactgcgtgt gacaaaagtg
     8461 aatgtcggcg gcgatcgcct gctggacatc gtctgcggtg cgccaaactg ccgtcagggc
     8521 ctgcgtgtgg cggtagcgac cattggtgct gttctgccgg gtgatttcaa aattaaagcg
     8581 gcgaaacttc gtggcgaacc gtctgaaggg atgctgtgct ccttctctga gctgggaatt
     8641 tctgacgatc ataacggcat aatcgagctg cctgcggatg cgccgattgg caccgacatc
     8701 cgcgaatacc tgaaactcga tgacaacacc atcgaaatca gcgtaacgcc aaaccgtgcc
     8761 gactgtttag gtatcattgg tgttgcgcgt gacgttgccg tgctgaatca gctgccgctg
     8821 gttgaaccgg aaatcgttcc ggttggtgcg accatcgacg acacgctgcc gattacagtc
     8881 gaagcgccgg aagcctgccc gcgttatctt ggccgtgtgg taaaaggcat taacgttaaa
     8941 gcgccaactc cgctgtggat gaaagaaaaa ctgcgtcgtt gcgggatccg ttctatcgat
     9001 gcagttgttg acgtcaccaa ctatgtgctg ctcgaactgg gccagccgat gcacgctttc
     9061 gataaagatc gcattgaagg cggcattgtg gtgcggatgg cgaaagaggg tgaaacgctg
     9121 gtgctgctcg acggcactga agcgaagctg aatgctgaca ccctggttat cgccgaccac
     9181 aacaaggcgc tggcgatggg cggcattttt ggtggcgaac actctggcgt gaatgacgaa
     9241 acgcaaaacg tcctgctgga atgcgctttc ttcagcccgc tgtctatcac cggtcgtgct
     9301 cgtcgtcatg gcctgcatac tgatgcgtct caccgttatg agcgtggcgt tgatccggca
     9361 ctgcagtaca aagcgatgga acgtgcgacc cgtctgctga tcgacatctg cggtggtgag
     9421 gctggtccgg taattgatat caccagcgaa gcaacgctgc cgaagcgtgc aaccatcact
     9481 ttgcgtcgta gcaaactgga tcgcctgatc ggccatcata ttgcggatga gcaggtaact
     9541 gacattctgc gtcgtctcgg ctgcgaagtg accgaaggca aagacgagtg gcaggcagtt
     9601 gcgccgagct ggcgtttcga tatggagatt gaagaagatc tggtcgaaga agtcgcgcgt
     9661 gtttacggct acaacaacat cccggatgag ccggtacagg caagcctgat tatgggtact
     9721 caccgtgaag ctgacctgtc gctcaagcgc gtgaaaacgc tgctcaacga caaaggctat
     9781 caggaagtga tcacctacag cttcgttgat ccgaaagtgc agcagatgat ccatccaggc
     9841 gttgaagcct tactgctgcc aagcccgatc tctgttgaaa tgtcagcaat gcgtctttct
     9901 ctgtggaccg gcctgctggc aaccgtggtg tacaaccaga accgtcagca gaaccgtgtg
     9961 cgcattttcg aaagcggtct gcgtttcgta ccagatactc aggcaccgtt gggcattcgt
    10021 caggatctga tgttagccgg tgtgatttgc ggtaaccgtt acgaagagca ctggaacctg
    10081 gcaaaagaga ccgttgattt ctatgatttg aaaggcgatc ttgaatccgt tctcgacctg
    10141 accggtaaac tgaatgaggt tgagttccgt gcagaagcga atccggcact gcatccgggg
    10201 caatccgcag cgatttatct gaaaggtgaa cgtattggtt ttgttggggt tgttcatcct
    10261 gaactggaac gtaaactgga tcttaacggt cgcactctgg tgttcgaact ggagtggaac
    10321 aagctcgcag accgcgtggt gcctcaggcg cgcgagattt ctcgcttccc ggcgaaccgt
    10381 cgtgacatcg cggtggtggt cgcagaaaac gttcccgcag cggatatttt atccgaatgt
    10441 aagaaagttg gcgtaaatca ggtagttggc gtaaacttat ttgacgtgta ccgcggtaag
    10501 ggtgttgcgg aggggtataa gagcctcgcc ataagcctga tcctgcaaga taccagccgt
    10561 acactcgaag aagaggagat tgccgctacc gtcgccaaat gtgtagaggc attaaaagag
    10621 cgattccagg catcattgag ggattgaacc tatggcgctt acaaaagctg aaatgtcaga
    10681 atatctgttt gataagcttg ggcttagcaa gcgggatgcc aaagaactgg ttgaactgtt
    10741 tttcgaagag atccgtcgcg ctctggaaaa cggcgaacag gtgaaactct ctggttttgg
    10801 taacttcgat ctgcgtgata agaatcaacg cccgggacgt aacccgaaaa cgggcgagga
    10861 tattcccatt acagcacggc gcgtggtgac cttcagaccc gggcagaagt taaaaagccg
    10921 ggtcgaaaac gcttcgccca aagacgagta atctgatcta actaaaaagg ccgcgttgcg
    10981 gccttttttc ttttcactgt cgaagagtca ccgtaaaatc aacgccatga cacttcagca
    11041 gaacggatac catgctgaca cttgcccgcc aacaacagcg acaaaatatt cgctggttat
    11101 tatgcctgtc agttttgatg ctgctggcgc ttctcttaag cctttgcgcc ggtgaacaat
    11161 ggatctcgcc aggtgactgg ttttctcctc gtggcgaact gttcgtctgg caaattcgcc
    11221 tgccacgtac gctggctgta ttgctggttg gtgcggcgct ggctatatcc ggcgctgtaa
    11281 tgcaggcgtt gtttgaaaat cctctggcag aacctggact acttggcgtc tctaacggcg
    11341 caggcgtggg gcttatcgcc gcggtattgc ttgggcaagg gcaactcccc aactgggcgc
    11401 tagggctgtg tgcgattgct ggcgcgctta tcatcacttt aatactctta cgtttcgccc
    11461 gtcgtcatct ttcgaccagt cggttattgc tggctggcgt tgcattaggg attatctgta
    11521 gcgcactaat gacgtgggct atctactttt ccacctctgt tgatttacgt cagctgatgt
    11581 actggatgat gggcggtttt ggcggcgtag actggcggca aagctggctg atgctggcat
    11641 tgatccccgt gttgttgtgg atctgttgtc agtccaggcc gatgaatatg ttagcacttg
    11701 gcgagatctc ggcgcggcaa ctgggtttac ccctgtggtt ctggcgcaat gtgctggtgg
    11761 cagcgaccgg ctggatggtt ggcgtcagtg tagcgctggc gggtgctatc ggctttattg
    11821 gtctggtgat cccacatatt cttcggttgt gtggtttaac cgatcatcgc gtattacttc
    11881 ccggctgcgc gctggcaggg gcgagcgcat tgctgctggc cgatattgta gcgcgcctgg
    11941 cattagctgc cgcagagctg cctattggcg tggtcaccgc aacgttgggt gcgccggtgt
    12001 ttatctggtt attgttaaaa gcaggacgtt agccgcaaaa agacggtcta tgattaaaag
    12061 ctaagatttt tactgaccac acccaggaga aacgatgcaa gattccattc tgacgaccgt
    12121 agtgaaagat atcgacggtg aagtgaccac gctggagaag ttcgccggta atgtgctgtt
    12181 gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa tatgagcagt tggagaatat
    12241 tcagaaagcc tgggccgatc gaggttttgt ggtgctggga ttcccgtgca accagtttct
    12301 ggaacaagaa ccgggcagcg atgaagagat taaaacttac tgtaccacca catggggggt
    12361 gacgttcccg atgttcagta agattgaagt taacggcgaa ggacgccatc cgctgtatca
    12421 aaaattgatt gccgcagcgc cgaccgcagt cgcgccggaa gagagcggat tctatgcccg
    12481 tatggtcagc aaaggtcgtg caccgctgta ccctgatgat attttatgga attttgagaa
    12541 atttttagtt ggcagggacg gattggtcat ccagcgtttt tccccggata tgacgccgga
    12601 agatcccatt gtgatggaaa gcattaaact ggcgttggca aaataatgtc tattgtgatg
    12661 cagttacagg atgttgcgga atctacccgc ctggggccgc tttctggcga gattcgggct
    12721 ggggagatcc tgcacctggt ggggccgaat ggcgcgggta agagtacctt actggcgcga
    12781 atggccggaa tgaccagcgg taagggaagc attcagttcg cggggcaacc actggaagca
    12841 tggtccgcaa caaaactcgc gctgcatcgc gcctatcttt cacaacagca gacgccgccg
    12901 tttgcaatgc cggtctggca ctacctgaca ctgcatcagc acgataaaac gcgtaccgaa
    12961 ctactgaatg atgtcgcagg ggcgctggct cttgatgaca aactcggtcg tagcaccaat
    13021 caactttccg gcggtgaatg gcaacgcgta cgtcttgctg cggtggtgtt gcaaatcaca
    13081 ccacaagcca atcccgcagg ccaattgctg cttcttgatg agccgatgaa cagtcttgat
    13141 gttgcgcaac aaagtgcgtt agacaaaatt ctgagcgctc tgtgtcagca aggactggcg
    13201 attgtgatga gcagtcacga tctcaaccac acgttgcgtc atgcgcatcg ggcgtggttg
    13261 ctaaaaggtg gaaaaatgct ggccagtgga cgcagagaag aggtgctcac gccgccaaat
    13321 ctggcgcagg cctatgggat gaattttcgc cgtctggata tcgaaggtca cagaatgctg
    13381 atttcgacca tctgatttca ttagtatctt gaaaatcggg taattacgac gctaaattaa
    13441 tacaagaata aaaaaacaga ggaaccgtac ggaatgcgtt tctgccttat tttgatcaca
    13501 gcactgtttc tggccgggtg tagccaccat aaagcaccgc cgccaaatgc cagactttct
    13561 gattcgatta ccgttattgc cggtttgaac gaccagctac aaagctggca tggcacgccg
    13621 tatcgttatg gtggcatgac gcggcgcggt gtggactgtt cgggatttgt ggtggtgacg
    13681 atgcgcgatc gttttgattt gcagctgccc cgagaaacca aagaacaagc ctctatcggc
    13741 acgcaaattg ataaagacga gttgctgcct ggtgacctgg tctttttcaa aacgggttcc
    13801 ggacaaaatg gtttgcatgt aggtatttat gataccaaca accaatttat ccacgcctct
    13861 accagcaagg gagtgatgcg ttcctcactt gataatgtct attggcagaa aaatttctgg
    13921 caggcgagac gaatctagta tgtcgaaaat tggacggcga tgatacatcg ccgtccattt
    13981 actccagaat acagtagtat tttgcagaca aaaattccgt aaatggatta ttcgtttata
    14041 atcaggataa tctaaaaaaa ggacccctga aaaagtggtt aacatggaac aaatgaaagt
    14101 tactccttct gtaattgtta tgtcttatgg cctcatccct tttagccgga tactgaaaaa
    14161 catccttcga gagggacggt taccatgaag atttttttgg agaatcttta tcattctgat
    14221 tgctatttcc tgccgataag agataaccag caagaccttg tcggcgttga gttaattacc
    14281 catttctcca gtgaagatgg tacggttcgt atcccgacga gtcgggttat agcgcaactt
    14341 accgaggagc aacattggca attattttca gagcagttgg aattactaaa atcatgccaa
    14401 cattttttta ttcaacataa actctttgcc tggcttaatt taacgccaca agtggctaga
    14461 ttattattag agagggataa ttacgcaggc gaactattaa aatatccgtt tattgaactg
    14521 ttgattaatg agaattaccc gcatctcaat gaaggaaaag ataatcgcgg tcttttatcc
    14581 ttatcgcaag tgtatcccct tgtgttaggt aatttgggag caggcaatag cacaatgaaa
    14641 gcggtttttg atggtctttt cacccgcgtc atgctggata aaagttttat tcagcaacaa
    14701 atcacgcacc gctccttcga accgtttatt cgtgcgatca aggcgcaaat ttccccttgt
    14761 tgcaattgca tcatcgcggg aggcattgat acggcagaaa tattggcaca gattaccccc
    14821 ttcgatttcc atgctctcca gggatgcctg tggcctgccg ttcccataaa tcagataacg
    14881 acgttggttc agcgataacc ctcctgtttg ctggtgttta agacgagagt aaccgtctac
    14941 actatcaaac aggaggatct atgaccctgt cttttattac ccgctggcgc gatgaattgc
    15001 cagcaaccta tacagcactt tcccctacgc ctttaaataa tgcccggctg atttggcata
    15061 ataccgaact cgctaacacg ctgagtattc catcgtcgct gtttaaaaat ggcgcaggtg
    15121 tctggggcgg cgaaacccta ctgcctggta tgtcaccact ggcgcaggtt tacagtggtc
    15181 atcagttcgg cgtctgggcg ggccaactgg gtgatgggcg cggcatttta cttggcgaac
    15241 aactgcttgc tgatggcact acaatggact ggcatctgaa aggtgctggc ctgacgcctt
    15301 attcgcgaat gggtgatgga cgggcggttt tacgttcgac gatacgagaa agtctcgcca
    15361 gtgaggcgat gcattatctg ggcattccga cgacccgcgc gttaagtatc gtcaccagca
    15421 attcgccagt gtatcgggaa acggtggagc caggcgcgat gctgatgcgt gtggcaccaa
    15481 gtcatctgcg ctttggccat ttcgaacatt tttactatcg acgcgagccg gaaaaggttc
    15541 gtcagttggc tgactttgcc attcgtcatt actggtcaca tcttgaagat gatgaggaca
    15601 aataccgtct ctggtttaac gatgttgtcg cacgtaccgc atcgttaatt gcccaatggc
    15661 agacggtcgg ctttgctcat ggggtgatga ataccgacaa catgtcgctg ctgggactga
    15721 cgcttgatta cgggccgttt ggttttctta atgattacga acccggtttt atttgtaatc
    15781 actcggatca tcaagggcgt tacagctttg acaatcaacc tgctgtcgcg ttgtggaatt
    15841 tacagcgtct ggcgcagaca ttgtcaccat ttgttgccgt agatgccctg aatgaggccc
    15901 tggacagcta tcagcaggtt ttgttgacgc attatggaca acggatgcgg cagaaactgg
    15961 gcttcatgac ggagcaaaaa gaggataacg cgctactgaa tgaattattc agtctgatgg
    16021 cgcgagagcg cagcgattat acccgcacat ttcgcatgct gagtctgacc gagcagcaca
    16081 gcgcggcgtc accgctacgt gatgagttta ttgatcgtgc ggcatttgat gactggtttg
    16141 cccgttatcg agggcgtttg caacaagacg aggttagcga tagtgagcgt cagcagctga
    16201 tgcaaagcgt taaccccgct ctggtattgc gcaactggtt ggcgcaacgg gcgattgaag
    16261 cggcagaaaa gggtgatatg acggaattgc accgcctgca tgaggcgttg cgaaatcctt
    16321 tcagcgacag agatgatgac tatgtcagtc gtccacctga ctggggtaaa cggctggaag
    16381 tcagctgctc gagttaaagg ctacttggtc aacagcagct tgccagcctg agttttacgg
    16441 agcagatatt cttgcccgtc atgatcgata atcagtttgc catccggacc taacagtgtc
    16501 tggctggaaa ttcgccgaat aggctgcggg gaagcggtct tgtgattggc atccgtttca
    16561 ggcgtgagtt ttctgctatc cgtataacgc ataaaaatta ataataattg atataacaat
    16621 gattcttatt atcaatatta tggctgacca gagcaagcag taatgtgttg agaagacaac
    16681 aagttgcgca gaaatgccca tgccgtcagg aatgggcacg cattcagaag cgggtatcta
    16741 ccgcagaggc gagtttttcg accaggcgtt cggtatcttc ccagcccaga cacgggtcgg
    16801 taatggattg accgtaagtg agcggctgac tgccgacgat tttttgcgtt ccttcgcgca
    16861 ggaaactttc cgccataatt ccagcaatcg ccgtagagcc attgcggatt tgctgacaaa
    16921 tatcctcaca aacttctaac tggcgacggt gctgcttctg gcagttaccg tggctgaaat
    16981 cgaccaccag atgttcaggt aaatcaaact cgtgcagcgt atcgcaggct gcggcgatat
    17041 catcggcatg ataattcggt tttttgccgc cacgcataat aatgtggcca tacgggttgc
    17101 cgctggtctg atagatggtc atctgaccat ttttgtctgg cgagaggaac atatggctgg
    17161 cgcgggctgc gcggatagca tccacagcaa tccgcgtgtt gccatcggta ccatttttaa
    17221 aacctaccgg acaggagagt gccgaagcca tttcacggtg gatctgactt tcggtagtac
    17281 gtgcgccaat cgcgccccaa ctgattaaat cagcaataaa ctgaccggtc accatatcga
    17341 ggaactcagt cgcggtaggg acacccagct catttacctg taaaagtaat ttgcgcgcca
    17401 gctccagacc gtgatttacc cgatagctgc cgtttaaatc tggatcggag attagtcctt
    17461 tccagccgac aacagttcgt ggtttttcaa aataggtgcg cattacgatt tccagccgtg
    17521 actggtactg gttgcgcagc gactgcagac gggtggcgta ctccattgca gcggtgagat
    17581 cgtggatcga gcaggggcca ataatgacca acagtcgctt atcttcacca ttgagtattt
    17641 tttcaattct gcggcgggag tcggtgacat gggtggcgac gccaggcgtt acgggatacc
    17701 gtagcgcgag ttcggcgggc gttaccaggc tctcaatacg cgcagtacgg agttcgtcag
    17761 ttctgttcat tacaagtctc agaaatttgt tgcttctacg gcaggactgc cgggaagtga
    17821 tgcgcatcac gataaaccaa tcgctgctga tttcaagtgc gaggtgtgtc acacctcgcc
    17881 gggtggttag catgataaca aaaaaataag ctaatgcact agttctctag tacattcggc
    17941 gactaaggcc catgatatcg aggatcttgg tggcaatctc ttctaccgaa taattggtac
    18001 tgttaatcca cgggatctga tttttacggt acaaggcttc cacttccgcg acttccatcc
    18061 tgcactgacg aagcgaggca tagcgactgt tctcccgacg ttcctcgcga atcgccgcca
    18121 gacgttccgg gtcgatagtc aggccgaaca atttatgctg aagcggtttg agcgacgcgg
    18181 gtagcaccag attatccata tcgtcggcaa taaaggggta gtttgccgcg cggataccaa
    18241 attgcattgc cagatacaga ctggtggggg ttttaccaca gcgagaaaca ccgagcagga
    18301 tcacctgagc ctgatccaga ttgcgcaacg aaatgccgtc atcgtgggcg agggtgtaat
    18361 caatcgccgc aatgcgcgca tcatatttat tgagattatt agggttaagg ccatgggtac
    18421 gatgagcaat cggcgttgga tccagtttca tctcttgttg tagcggggca accagcgcct
    18481 gaacgatatc ctggcaaaag ccttcacttt gcaagatgat ggcgcgaatc tccggcaaca
    18541 cgatggagta gaagaccagc gggcgcacgc ctgtctggtg ataaattgcg tcaatctgat
    18601 ccttcactgc ccgtgcacgg ctctcatttt cgacaaacgg cagcgtgatg ctgctgatag
    18661 tgacgggaaa ttgtgacatt actgcgtgtc ctaatacctc cgcagttatt gccgtaccat
    18721 cagaaatata aaaaacgtgg cgatcaacag cattatccat tttgttcttc ccgtgatgca
    18781 gacataattc tatgaaagca taaattaaaa acgcac
//
LOCUS       DAZGBC010000093        18410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGBC010000093 DAZGBC010000000
VERSION     DAZGBC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.093"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..634)
                     /gene="rsmG"
                     /locus_tag="ACHK1J_003123"
     CDS             complement(11..634)
                     /gene="rsmG"
                     /locus_tag="ACHK1J_003123"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO7912362.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(698..2587)
                     /gene="mnmG"
                     /locus_tag="ACHK1J_003124"
     CDS             complement(698..2587)
                     /gene="mnmG"
                     /locus_tag="ACHK1J_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO7912363.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2966..3409)
                     /gene="mioC"
                     /locus_tag="ACHK1J_003125"
     CDS             complement(2966..3409)
                     /gene="mioC"
                     /locus_tag="ACHK1J_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO7912364.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3499..3957)
                     /gene="asnC"
                     /locus_tag="ACHK1J_003126"
     CDS             complement(3499..3957)
                     /gene="asnC"
                     /locus_tag="ACHK1J_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO7912365.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4109..5101
                     /gene="asnA"
                     /locus_tag="ACHK1J_003127"
     CDS             4109..5101
                     /gene="asnA"
                     /locus_tag="ACHK1J_003127"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO7912366.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5106..6557)
                     /gene="viaA"
                     /locus_tag="ACHK1J_003128"
     CDS             complement(5106..6557)
                     /gene="viaA"
                     /locus_tag="ACHK1J_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO7912367.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSKEWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6551..8047)
                     /gene="ravA"
                     /locus_tag="ACHK1J_003129"
     CDS             complement(6551..8047)
                     /gene="ravA"
                     /locus_tag="ACHK1J_003129"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO7912368.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQACAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8270..10138
                     /gene="kup"
                     /locus_tag="ACHK1J_003130"
     CDS             8270..10138
                     /gene="kup"
                     /locus_tag="ACHK1J_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO7912369.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10305..10724
                     /gene="rbsD"
                     /locus_tag="ACHK1J_003131"
     CDS             10305..10724
                     /gene="rbsD"
                     /locus_tag="ACHK1J_003131"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO7912370.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10732..12237
                     /gene="rbsA"
                     /locus_tag="ACHK1J_003132"
     CDS             10732..12237
                     /gene="rbsA"
                     /locus_tag="ACHK1J_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO7912371.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12242..13207
                     /gene="rbsC"
                     /locus_tag="ACHK1J_003133"
     CDS             12242..13207
                     /gene="rbsC"
                     /locus_tag="ACHK1J_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO7912372.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13232..14122
                     /gene="rbsB"
                     /locus_tag="ACHK1J_003134"
     CDS             13232..14122
                     /gene="rbsB"
                     /locus_tag="ACHK1J_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO7912373.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14248..15177
                     /gene="rbsK"
                     /locus_tag="ACHK1J_003135"
     CDS             14248..15177
                     /gene="rbsK"
                     /locus_tag="ACHK1J_003135"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO7912374.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLKSVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15181..16173
                     /gene="rbsR"
                     /locus_tag="ACHK1J_003136"
     CDS             15181..16173
                     /gene="rbsR"
                     /locus_tag="ACHK1J_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO7912375.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16139..17566)
                     /gene="mdtD"
                     /locus_tag="ACHK1J_003137"
     CDS             complement(16139..17566)
                     /gene="mdtD"
                     /locus_tag="ACHK1J_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO7912376.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17589..18281)
                     /gene="yieP"
                     /locus_tag="ACHK1J_003138"
     CDS             complement(17589..18281)
                     /gene="yieP"
                     /locus_tag="ACHK1J_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO7912377.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4432 a         4565 c         4818 g         4595 t
ORIGIN      
        1 ttgataaaaa ttaaatttta tttgctttaa tcaccaccag atggcgttcg ccatccaggg
       61 caggaacctg aagtttaacc actgattcaa cctgatattc ttcgggcaac aaagcgattt
      121 catcttccgg catttgccct ttcagcgcgt agaaacggcc ttgctcacca ggaagatggt
      181 ggcaccagct caccatatcg ttcagagagg caaaagcgcg gctaattacg ccatcaaatg
      241 gcggctctga aggaaactct tctaccctgc tctgtactgg ctcaatattc tccagtttaa
      301 gctcatgttg cacctgacga aggaaacgca cgcgtttacc aaggctatcc aacagagtga
      361 aatgggcttc aggacgcacg atagagagtg gaatgccagg cagtccagga ccggtgccga
      421 catcgataaa ccgttcacct tgcagatacg gtgccaccac aatgctatcg agaatatggc
      481 gtaccagcat ctcattagga tcgcggaccg aagtcaggtt gtacgctttg ttccatttat
      541 gcagcatatt cacgtaggca ataagctggt ttttctggtg atcggtaagc gaaatacctg
      601 cgtctttcag cagtaaggag agtttgttga gcacggtgat tacctgttct tgatgcgttg
      661 cctggtaagc gggtgcttac caggcatttt taatgcgtta tgcgctacga cgcagcatac
      721 cctgtttttt cagccacacc agcagaatgg agatggccgc aggcgtgacg ccagaaatac
      781 gtgaagcctg gccgatagag gccggtttgt gatcgttaag tttggcgatc acttcgttag
      841 aaagaccgga tacctggcgg taatccagtg tcgcgggtag cagggtgttc tcgttacgca
      901 gctgcttttc gatctcatct tgctggcgcg cgatataacc ttcgtattta acctgaatct
      961 caacctgttc cgccgcctgt tcgtctgtca acgcaggggc aaacggcgtc agcgtggtta
     1021 atttttcata agtcatttcc ggacgacgca gcagatcttc accactggct tcacgggaaa
     1081 gcggcgcagt caggtgagca ttcacttcgg ctgcagcttc cgccgacggg gttacccagg
     1141 tcgatttcag acgctgacgc tcacgctcga tattctcaag tttctcgtta aagcgcgccc
     1201 aacgttcgtc atccaccagg cccagttcac gaccgatttc agtcaaacgc agatccgcat
     1261 tatcttcgcg cagcatcaga cgatattctg cgcgcgaagt aaacatacga tacggttctt
     1321 tggttcctaa agtgcacagg tcatcaacca gtacgccgag ataggcctga gaacgcgccg
     1381 gagcccaacc ttctttgtca gcagacagac gggcagcgtt aagaccggcc agcaaacctt
     1441 gcgcagcggc ttcttcgtaa ccggtagtgc cgttaatctg accagcaaag aacagcccct
     1501 ggataaactt gctctccagc gtcggtttca ggtcgcgtgg atcgaagaag tcatactcaa
     1561 tggcataacc cggacgcacg atcttcgcgt tttccatccc ctgcatggag cggacgattt
     1621 gcatctgcac atcgaacggc aggctggtgg agataccgtt cggataaatt tcgttagagg
     1681 tcagcccttc cggttcaagg aagatctgat gctgatttct gtcggcgaag cgcatgactt
     1741 tgtcttcgat cgacgggcag tagcgtgggc cgacaccttc gatcacccct gcgtacattg
     1801 ggctacgatc gaggttactg cggatcacat catgggtttt ctcgttggta tgagtgatat
     1861 aacacggcac ctgctgggga tgctgggacg cattgcccat aaacgagaat accggcattg
     1921 ggttatcgcc atgctgttgc gcaagcacgc taaagtcgat ggttcgagca tcaatacgcg
     1981 gtggtgtccc ggttttcaga cgaccaacgc gcagcggaag ttcacgcaaa cggcgagaaa
     2041 gcggaatgga cggcggatca ccagcacggc caccgctgta gttatccaga ccgatatgaa
     2101 ttttaccgtc gaggaacgtc ccaacggtga gcacaacggc tttggcacgg aacttcagtc
     2161 ccatttgggt aacagcaccg accacgcgat cgttttcgac aataagatct tcaaccgcct
     2221 gctggaagat catcagattc ggttggttct ccagcgccgt acgtaccgcc tgacggtaga
     2281 gcacacgatc cgcctgagct cgggtagcgc gaacagccgg tcctttactt gcgtttagta
     2341 tcctaaactg gatacccgcc tgatcgatcg ctttcgccat cagaccgccg agtgcatcca
     2401 cttcttttac cagatgtccc ttcccaatac cgccgatcgc cgggttgcag ctcatctgcc
     2461 ccagagtgtc gatattgtgt gtcaaaagca gagtctgttg acccatacgc gccgcagcca
     2521 tcgcggcctc ggtgcctgca tgacccccgc caatgatgat gacgtcaaaa ggatccggat
     2581 aaaacatggt gattgcctcg cataacgcgg tatgaaaatg gattgaagcc cgggccgtgg
     2641 attctactca actttgttgg cttgagaaag acctgggatc ctgggtatta aaaagaagat
     2701 ctatttattt agagatctgt tctattgtga tctcttatta ggatcgcact gccctgtgga
     2761 taacaaggat ccgactttta agatcaacaa cctggaaagg atcattaact gtgaatgatc
     2821 ggtgatcctg gaccgtataa gctgggatca gaatgagggg ttatacacaa ctcaaaaact
     2881 gaacaaccgt tgttctttgg ataactaccg gttgatccaa gcttcctgac agagttatcc
     2941 acagtagatc gcacgatctg tatacttatt tgagtaaatt aatccatgat cccagccatt
     3001 cttctgccgg atcttccgga atgtcgtgat caagaatgtt gatcttcagt gtttcgcccg
     3061 tctgttttgc accggaattt ttgagttcag cctcgagttt atcgatagcc ccacaaaagg
     3121 tgtcatattc acgactacca ataccgattg cgccaaagcg gaccgcagaa agatcgggct
     3181 tctgttcctg caatgcttca tagaaaggag aaaggttgtc cggaatatct ccggcaccgt
     3241 gggtggagct gataaccagc cagatccccg aggcagataa atcttccaac agcggaccgt
     3301 gcagcgtttc ggtggtaaaa cccgcctctt ccagcttttc agccaggtgt tctgctacat
     3361 attcggcacc gccgagggtg ctgccgctga taagagtgat atctgccata aaccgccacc
     3421 tttattaaga gtggcgtatt gtacgctgtg aacgcgttgg gatctacctg tggaaaagta
     3481 tgggattaaa aaagccgatc agggcttgat ggtacgcatg atcgggttct gcaggacgat
     3541 cagtgtctcg gtggactgaa tttcatcaat tgtttggatc ttgttgataa gtacatgctg
     3601 gagagcatcg atcgaacggc acatcacttt tataaagatg ctgtagtggc cggtggtgta
     3661 gtacgcctcg gtcacttcat cgaggctttc cagctttgcc agcgcggaag gatagtcttt
     3721 ggcgctcttt aatataatgc cgataaagca gcctacgtca taaccgagct gcttcgggct
     3781 gacatcaata cgcgccccgg taatgattcc cgcctgcttc attttctcta ctcgaacgtg
     3841 aatcgtcccc ggactgacgc caaattgttt cgccagttcg gcgtaagcgg tgcgcgcatt
     3901 gcccattaat gcttccagga tgccacggtc cagattgtcg atcagataat tttccatagg
     3961 attttcttat gcggattgat gattcattct attttagcct tcttttttaa tgaatcaaaa
     4021 gtgagttagg ctttttattg aatgattatt gcatgtgtat cggtttttgt tgcttaatca
     4081 taagcaacag gacgcaggag tataaaaaat gaaaaccgct tacattgcca aacaacgcca
     4141 aattagcttc gtgaaatctc acttttctcg ccaactggaa gaacgtctgg ggctgatcga
     4201 agtccaggcg ccgattctta gccgtgtggg ggatggcacg caggataact tgtcgggctg
     4261 tgaaaaagcg gtgcaggtaa aagtgaaagc tctgcctgat gcccagttcg aagtggttca
     4321 ttcactggcg aagtggaaac gtcagacctt agggcaacac gacttcagcg cgggcgaagg
     4381 gctgtacacg cacatgaaag cccttcgccc cgatgaagac cgtctttctc cgttgcactc
     4441 ggtctatgtt gaccagtggg actgggaacg cgtaatgggc gacggtgagc gtcaattctc
     4501 gactctgaaa agcacggtag aggcgatctg ggcgggaatt aaagcaaccg aagctgcggt
     4561 tagcgaagag tttggcctgg caccgttcct gccggatcag atccacttcg tacacagcca
     4621 ggagttactg tctcgttatc cggatcttga tgccaaaggg cgtgagcggg cgatagcgaa
     4681 agatcttggt gcggtattcc ttgtcgggat tggcggcaag ctgagcgatg gtcatcgtca
     4741 cgacgtgcgc gcaccggatt atgatgactg gagcaccccg tcagagctgg gccatgcggg
     4801 cctgaacggc gatattctgg tgtggaaccc ggtactggaa gatgcgtttg agctttcttc
     4861 catggggatc cgcgtggatg ccgacacgtt gaagcatcag ttggcgctga ccggtgatga
     4921 agatcgtctg cagctggaat ggcatcaggc gttgctgcgc ggtgaaatgc cgcagaccat
     4981 cggcggcggt atcggccagt ctcgtttgac catgctgctg ctgcaactgc cgcatatcgg
     5041 ccaggttcag tgtggagtat ggccagctgc tgttcgcgag agcgtccctt ctctgctgta
     5101 ataatttatc gccgccagcg tctgagcagg cggcttcgca tcccggtatc aaagcgccag
     5161 atatgatcga aaatgcgcat gatgccgggt ttgccgtgtg ccgacatcgc cacggcatga
     5221 aagcgatgct gatgtacccg ctgcagctct ttcactttac tcgtcacgtc gtcaggcaac
     5281 cgctgggcga taaaatcaga aatcaccacc gcatcggcat caaaccattc cttgctttgc
     5341 aagcgttcca taatggcgcg aaaacaactg gcaagatcgg tgccgccacg aaactgctgg
     5401 cttaaaaaac ggattgcttg ttcgatgcct tgtgggcctg aaagctcata acggacgatc
     5461 tcggtggaaa atagcataat ataacagcgc cggttttcag cgagagcaat gcgcatcaaa
     5521 gccaggcaga acgctttcgc acactgttca ttaaagccgc ccattgagcc ggaagtatcc
     5581 acgcagacaa taaacggtcc gcgcggctgt tcgtcgtaat ctttatgcac caccgggcgt
     5641 tcgatcactt tttcacgcca cgactcaccg tgcaggcgat aggtgagcaa ctgtttttcc
     5701 accagccgac ggtaaaactc atactccagt tccgttatcc ctagtgtcgc cagttctggc
     5761 ggcagaagac gtaaaatatc atcgctttgt tgcagaccat caacctgctc aggaacagtt
     5821 gccggttcgc gcaccatggt gcggaaggtt tccatctgcg catcgttgcg cggtattgat
     5881 ttggcttccc gggaacgccc cagctgttct gccaggcgtt tcagttccgg ctgttcgtta
     5941 agaaattcac cgtatttcac aatcaactga tagtcgccac gtttaagctg accggcgctc
     6001 atatcccaca gacgaccagc tgcggtattg ttatctgcga gaatcggttc aagttgtccg
     6061 ctcagcgtca tgcgttcctg aacttcactc aacagttgtt cgcgttcttc ttctaataac
     6121 tgttgattta acgtcgttgc ttgcacgatc agacttaaac gccaacgctg gaggaaaagc
     6181 gtgtgtaacg ctgaagtgat cgtgctgtta gcatcaacca actgtcgggc ttgttctgcc
     6241 catggggaat tcagacgatg cagtaagtcc aggatctgtg gtagctgcac aataaactgc
     6301 ggcgtggaga ggagctggct ttgctgatag cacatcacct cttcggtgag ttccggcggg
     6361 actcgggcat ctttcagccg attacgcagc gcctcacgcc agcggggaac atcatcagtg
     6421 attgccgcct tcagtcgtgg gaatttttca aagaagaccg ctagctgcgg tgaggccagc
     6481 agcgcgatga tcatctcttc gatcaatccc tcttcgctga cggccagcat cacattaagc
     6541 gtatccagcg ttagcattgt tgtgcctggc gaatctgtgc gccgacatcc tgtaggctgg
     6601 cttcaatgcg acctaaccag tcgccaggga taaacagaca tttttgctgt tcgctgaata
     6661 aagcgtgttg cttacgccag tcgctttcaa gctcttccag ttgttgtttt atttcacctg
     6721 gcagaccttc agccgatgaa ccgggaagag ccagcgtact gccttgtaaa cttacatcgc
     6781 gtacaacaag atgttgggtg ctatcaactt ccagattcag tttctgggca aagccgatac
     6841 cgttcagttt gccgcgaatt tcaccacctt tactcagcca ctgttccagc gcgctacgct
     6901 caaaggagat atgaaccact tccatatcat gcagttttaa cggtttttgc agcagcagag
     6961 tcagagtgga agcagtaacg ttaacaggga gttgatactg ctgacgacgg ctgaaaatgc
     7021 cgcccagacg aattaccgtt aaggctgttt tatcactttg ttgctgctgg agttgcaggt
     7081 gacgttgcac aatcgcgcca aggcgagtca acatcccttg ctgttgccag gcgtgaccgg
     7141 tcatcaatac atcaatttgt tgttgtatca aattcaggct ttgcgcgtca taccacaggc
     7201 aatctttcag caaaatgaga tctaccgggg caacagcact gcgaccgcta aaaaaggcgc
     7261 aggcctgcaa taagcggatc gcttttttcc agcgacgatc cgagacataa ggcgcatccg
     7321 gtaatttatc cagttgctgg cgcagcataa aaatcagctc aaatacatga tcgggcagcg
     7381 taatttcacc aatctctttc tgccagcgtt catattcttc atctgtgacc tgcaaggcat
     7441 caggaaccgg attgtcgttt tcatcctgtt gactggtcag catggagcgg aaattcgctt
     7501 tatcctgcac tttatctaac cacagacgaa tcagcatgcg gtcatataac gcttccagac
     7561 tgctgtctgc ttccggcagc tcgttggagg ccgccaccag tagacgcatc gggatttttt
     7621 ctacgtgtgc accgttgcgg aactggcgct cgttaatggc ggtgagcaag gtattaagaa
     7681 ttgccgggcc cgctttccag atctcatcca gaaagacaat ttctgcttcc ggcaggtaac
     7741 cgctggttaa acgttcatag cgcccttcat cttttagcgc ctgaatagaa aggggaccaa
     7801 aaacttcttc cggcgtggag aagcgagtca tcagatattc aaacgcgcgg gcattctgaa
     7861 aggcgaattt taagcgccgg gcgatcaaac ttttggcaat acctggcggg ccaaggagga
     7921 acacactttc accacttaat gccgctaata aacacaagcg gatggcgtgg ctacgttcat
     7981 aaagcccctt ttccaacgaa ctgctcaggc gggaaattct ttccgctaat aaatgagggt
     8041 gagccataat gaagtggcgt cctttcgtca aaagttctgc gtaaattgcg agtatagacg
     8101 tttcttgctg gtggctaaaa tagtctcaaa ggggggggat ttttctttga gccaggttaa
     8161 tgtggccgca tttaggagta cgattttgcc gttaatcgtg catactgtgc gcttttttgt
     8221 gggccaaggg actaagcaca catttcatat ttcaacgaaa gactagtcta tgagcactga
     8281 taataagcaa tcattgcccg cgattaccct cgcggcgatt ggagttgtct acggcgatat
     8341 tggtaccagc ccgttatata cacttcgtga atgtttgtcc ggccagtttg gttttggcgt
     8401 tgaacgcgat gccgtgtttg gctttttatc gctgatcttc tggctgctaa tctttgtggt
     8461 ttccattaaa tatctcacct tcgtgatgcg ggcagataac gccggtgagg gggggatcct
     8521 gacgttgatg tcgcttgccg ggcgtaatac gtcggcgcga accacatcaa tgctggtgat
     8581 tatggggcta atcggcggca gttttttcta tggtgaagtc gtcataacac ccgctatttc
     8641 ggtgatgtca gccattgaag gtctggaaat cgtcgccccg cagctggata cctggatagt
     8701 tcccctctca attatcgttc tcactttatt atttatgatt caaaagcatg gcacggctat
     8761 ggtcggtaag ctgtttgcgc cgatcatgct gacctggttt ttgattctgg caggactggg
     8821 gttacgtagc attattgcta acccggaagt gctgcatgcg ctgaatccga tgtgggcggt
     8881 gcatttcttc cttgaataca aaacggtttc ttttattgca ttaggggcag tggtgctgtc
     8941 gattacgggg gtcgaggcgc tgtatgctga tatgggacac tttggtaagt tccctattcg
     9001 tctggcgtgg ttcactgtcg tattaccttc cttaactctt aattacttcg gccagggagc
     9061 gctgttgtta aagaacccgg aagcgattaa gaacccgttc ttccttttgg caccggactg
     9121 ggcgctgatc ccgctgctga tcatcgccgc actggcgacg gtaatcgcct cgcaggcggt
     9181 tatctctggc gtcttttcat tgacgcgtca ggcggtacgt ctgggatatt tgtcgccgat
     9241 gcgcattatt cacacctccg aaatggagtc agggcaaatc tatattccgt ttgtgaactg
     9301 gatgctctat gtcgcggtcg tgattgtgat tgtcagcttt gagcactcca gcaacctggc
     9361 ggcggcgtac gggattgcgg tgaccggaac catggtgctg acgtctattc tctcgactac
     9421 cgtggcacgt cagaactggc actggaataa gtattttgtt gcgctgatcc tgattgcttt
     9481 cctttgtgtc gatattccat tgttcaccgc taacctcgat aaactgctct ccggcggctg
     9541 gttgccattg agcctcggta ctgtgatgtt tatcgtgatg accacctgga agagcgagcg
     9601 tttccgcttg ctgcggcgga tgcatgaaca tggtaactct ctggaagcga tgattgcttc
     9661 gctggagaaa tcaccgcctg ttcgcgtgcc cgggaccgcg gtgtatatgt cgcgtgcaat
     9721 caacgtcatt ccctttgcgc tgatgcataa cctcaaacat aacaaggtat tgcatgagcg
     9781 ggtgattctg ttaactctgc gcaccgaaga cgcgccatat gtccataacg tccgtcgggt
     9841 acagattgaa caactgtcgc ccactttctg gcgcgtggtg gcaagttatg gttggcgaga
     9901 aacgccaaac gtggaagaag ttttccaccg ctgcggtctg gaaggattaa gttgccggat
     9961 gatggaaacc tccttcttta tgtcgcatga gtcgttgatc ctcggcaaac gcccgtggta
    10021 tttgcgtctg cgcggcaagc tgtacttgct gctgcaacgt aatgcgctgc gtgcaccaga
    10081 tcaatttgaa atcccgccaa acagggttat cgaactgggt actcaggtcg aaatctaacg
    10141 ccagacgcct cctttcttca taagggggcg tttttgtttt catggttaat caccatgtaa
    10201 aacgtttcga ggttgatcac atttccgtaa cgtcacgatg gttttcccaa ctcagtcagg
    10261 attaaactgc gggtcagcga aacgtttcgc tgatggagaa aaaaatgaaa aaaggcaccg
    10321 ttcttaattc tgatatttca tcggtgatct cccgtctggg acataccgat acgctggtgg
    10381 tgtgtgatgc tgggttaccc atccccaaaa gtacaacgcg tatcgatatg gcattaaccc
    10441 agggtgtacc ttcttttatg caggtgctgg gcgtcgtcac aaatgaaatg caggtcgagg
    10501 cggccattat cgcggaagag atcaaacaac ataatccgca actccacgaa acgttgctca
    10561 ctcaccttga gcagctgcaa aaacaccagg gaaataccat tgaaattcgt tacaccacgc
    10621 atgaacaatt caaacaacaa accgcagaaa gtcaggcggt aattcgcagc ggagaatgtt
    10681 ctccgtatgc gaatatcatt ctctgtgctg gcgtgacgtt ctgaggccgt catggaagca
    10741 ttacttcagc ttaaaggcat cgataaagcc ttcccgggcg taaaagccct ctcgggcgct
    10801 gcgttaaatg tctatccggg ccgcgtgatg gcgctggtgg gcgaaaacgg cgcgggtaaa
    10861 tccaccatga tgaaagtgct tactggtatc tatactcgcg atgccggcac gcttttatgg
    10921 ctggggaaag aaacgacatt taccgggccg aaatcttccc aggaagccgg gattgggatt
    10981 atccatcagg aactgaacct gatcccgcag ttgaccattg ccgaaaacat tttcctcggt
    11041 cgtgagtttg ttaatcgctt tggcaaaatt gactggaaaa ccatgtatgc cgaagcggat
    11101 aaattgctgg ctaaacttaa cctgcgcttt aaaagcgaca agctggtggg cgatctttcc
    11161 atcggtgacc agcaaatggt tgaaatcgcc aaagtgctga gctttgagtc gaaagtcatc
    11221 attatggatg aaccgaccga tgcgctgacc gataccgaaa ccgaatccct gttccgcgtc
    11281 atccgcgagc tgaaatcgca aggccgcggt attgtctata tctcccaccg catgaaagaa
    11341 atcttcgaga tttgcgatga cgttaccgtt tttcgtgacg gacaatttat tgctgagcgc
    11401 gaagtggcat cactgaccga agattcgctg attgagatga tggtgggtcg caagctggaa
    11461 gatcaatatc cgcacctgga caaagcgccg ggagatatcc gcctgaaagt cgataatctc
    11521 tgcggacctg gcgttaacga tgtctctttt actttacgca aaggcgaaat tcttggcgtc
    11581 tctggtttga tgggcgcagg tcgtaccgaa ctgatgaaag tgctctacgg cgcactaccg
    11641 cgcaccagcg gttacgtcac cctggatggg catgaagtcg ttacccgttc accgcaggat
    11701 ggcctggcaa acggcattgt gtatatctcc gaagaccgta aacgtgacgg tttagtgttg
    11761 ggcatgtcag taaaagagaa catgtcgctg acagcgctgc gctacttcag ccgcgctggt
    11821 ggcagtttga agcatgccga tgaacagcag gctgtgagtg atttcattcg tctgtttaat
    11881 gtgaaaactc cgtcgatgga acaggcaatt ggtctgcttt ccggtggcaa tcagcaaaaa
    11941 gtggcgattg cccgcggtct gatgacacgc cccaaggtgt tgatcctcga tgagcctacc
    12001 cgtggcgtag atgtcggcgc gaaaaaagag atctatcaac tgattaacca gttcaaagcc
    12061 gatggcttga gcatcattct ggtgtcatcg gagatgccag aagtattagg catgagcgat
    12121 cgcatcatcg tcatgcatga agggcatctc agcggggaat ttactcgtga gcaggccacc
    12181 caggaagtgt taatggctgc cgctgtgggc aagcttaatc gcgtgaatca ggagtaaaaa
    12241 aatgacaacc cagactgtct ctggtcgccg ttatttcacg aaagcgtggc tgatggagca
    12301 gaaatcgctt atcgctctgc tggtgctgat cgcgattgtc tcgacgttaa gcccgaactt
    12361 tttcaccatc aataacttat tcaatattct ccagcaaact tcggtgaacg ccattatggc
    12421 ggttgggatg acgctggtga tcctgacgtc gggcatagac ttgtcggtag gttctctgtt
    12481 ggcgctaacc ggcgcagttg ctgcatctat cgtcggcatt gaagtcaatg cgctggtggc
    12541 tgtcgctgcc gctctcgcgt taggtgccgc aattggtgcg gtaaccggag tgattgtagc
    12601 gaaaggtcgc gtccaggcgt ttatcgctac gctggttatg atgcttttac tgcgcggcgt
    12661 gaccatggtt tataccaacg gtagcccagt gaataccggc tttactgaga acgccgatct
    12721 gtttggctgg tttggtattg gtcgtccgct gggcgtaccg acgccagtct ggatcatggg
    12781 gattgtcttc ctcgcggcct ggtacatgct gcatcacacg cgtctggggc gttacatcta
    12841 cgcgctgggc ggcaacgaag cggcaacgcg tctttctggt atcaacgtca ataaaatcaa
    12901 aatcatcgtc tattctcttt gtggtctgct ggcatcactg gccgggatca ttgaagtggc
    12961 gcgtctctcc tccgcacaac ccacggcggg gactggctat gagctggatg ctattgctgc
    13021 ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg ggacgttgat
    13081 cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg tttcctccta
    13141 ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag acaacaaaaa
    13201 gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg ctaccctggt
    13261 ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag acaccatcgc
    13321 gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg gcgcgcagaa
    13381 agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca acccggcgaa
    13441 agagctggcg aacgtgcagg acttaaccgt tcgcggcaca aaaattctgc tgattaaccc
    13501 gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga acatcccggt
    13561 tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg catctgataa
    13621 cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg aaggtgccaa
    13681 agttatcgag ctgcaaggca ttgctggtac atccgcagcc cgtgaacgtg gcgaaggctt
    13741 ccagcaggcc gtagctgctc acaagtttaa tgttcttgcc agccagccag cagattttga
    13801 tcgcaccaaa ggtttgaacg taatgcagaa cctgttgacc gctcatccgg atgttcaggc
    13861 tgtattcgcg cagaatgatg aaatggcgct gggcgcgctg cgcgcactgc aaactgccgg
    13921 taaatcggat gtgatggtcg tcggatttga cggtacaccg gatggcgaaa aagcggtgaa
    13981 tgatggcaaa ctagcagcga ctatcgctca gctacccgat cagattggcg cgaaaggcgt
    14041 cgaaaccgca gataaagtgc tgaaaggcga gaaagttcag gctaagtatc cggttgatct
    14101 gaaactggtt gttaagcagt agttttaatc aggttgtatg acctgatggt gacataaata
    14161 cgtcatcgac agatgaacgt gtaatataaa gaaaagcagg gcacgcgcca ccctaacacg
    14221 gtggcgcatt ttatggacat cccgaatatg caaaacgcag gcagcctcgt tgttcttggc
    14281 agcattaatg ctgaccacat tcttaatctt caatcttttc ctactccagg cgaaaccgta
    14341 accggtaacc actatcaggt tgcatttggc ggcaaaggcg cgaatcaggc tgtggctgct
    14401 gggcgtagcg gtgcgaatat ctcctttatt gcctgtactg gcgatgacag cattggcgag
    14461 agcgttcgcc agcagctcgc cactgataac atcgatatta ctccggtcag cgtgatcaaa
    14521 ggcgaatcaa caggtgtggc gctgattttt gttaatggcg aaggtgagaa tgtcattggt
    14581 attcatgccg gcgctaatgc tgccctttcc ccggcgctgg tggaagcgca acgtgagcgt
    14641 attgccaacg cgtcagcatt attaatgcag ctggagtcac cactcaaaag tgtgatggca
    14701 gcggcgaaaa tcgcccatca aaataagact atcgttgcgc ttaacccggc tccggctcgc
    14761 gaacttcctg acgaactgct ggcgctggtg gacattatta cgccaaacga aacggaagca
    14821 gaaaagctca ccggtattcg tgttgaaaat gatgaagatg cagcgaaggc ggcgcaggta
    14881 ctgcatgaaa aaggtatccg tactgtactg attactttag gaagtcgtgg tgtatgggct
    14941 agcgtgaatg gtgaaggtca gcgcgttcct ggattccggg tgcaggctgt cgataccatt
    15001 gctgccggag atacctttaa cggcgcgtta atcacggcgt tgctggaaga aaaaccattg
    15061 ccagaggcga ttcggtttgc ccatgctgcc gctgcgattg ccgtaacacg taaaggcgca
    15121 caaccttccg taccgtggcg tgaagagatc gacgcatttt tagacaggca gaggtgacgc
    15181 ttggctacaa tgaaagatgt tgcccgcctg gcgggcgttt ctacctcaac agtttctcac
    15241 gttatcaata aagatcgctt cgtcagtgaa gcgattaccg ccaaagttga agcggcgatt
    15301 aaagaactca attacgcgcc atcagctctg gcgcgtagcc tcaaactcaa tcaaacacat
    15361 accattggca tgttgatcac tgccagtacc aatcctttct attcagaact ggtgcgtggc
    15421 gttgaacgca gctgcttcga acgcggttat agtctcgtcc tttgcaatac cgaaggcgat
    15481 gaacagcgga tgaatcgcaa tctggaaacg ctgatgcaaa aacgcgttga tggcttactg
    15541 ttactgtgca ccgaaacgca tcagccttcg cgtgaaatca tgcaacgtta tccgacagtg
    15601 cctaccgtga tgatggactg ggctccgttc gatggcgaca gcgatcttat tcaggataac
    15661 tcgttgctgg gcggagactt agcaacgcaa tatctgatcg ataaaggtca tacccgtatc
    15721 gcctgtatta ccggcccgct ggataaaact ccggcgcgcc tgcggttgga aggttatcgg
    15781 gcggcgatga aacgtgcggg tctcaacatt cctgatggct atgaagtcac tggtgatttt
    15841 gaatttaacg gcgggtttga cgctatgcgc caactgctat cacatccgct gcgtcctcag
    15901 gccgtcttta ccggaaatga cgctatggct gttggcgttt accaggcgtt atatcaggca
    15961 gagttacagg ttccgcagga tatcgcggtg attggctatg acgatatcga actggcaagc
    16021 tttatgacgc caccattaac cactatccat caaccaaaag atgaactggg ggagctggcg
    16081 attgatgtac tcatccatcg gataacccag ccgacccttc agcaacaacg attacaactt
    16141 actccgattc tgatggaacg cggttcggct tagatttacg ctgtcttttg atcaaattat
    16201 taccatcggt tgttttcaga agcatgaaca ttgctgctga agcaacagta ataatgccca
    16261 tcgtgataaa cgtatagtgg aattgttcga cagtcgttgt gccttccatc ccttcataaa
    16321 cgcgaaggac ggccgcactt acagcaacgc ctaaactgat agacagttgc tgcgtgaccg
    16381 ccagaacact gttaccgctg ctggcgttgt catcggtcag atcggcaagt gtgatggtat
    16441 tcatcgcggt aaactgcgtc gacatagcca tccctaatat aaacaacggc aagatcagca
    16501 tccatatcgc cattgccggt gattgcaaag agaactgagc gatcattagc ccaataatca
    16561 ccgtgatccc cactaatgta tggcgatagc ccagacgacg taagacttgg gtaaccatcg
    16621 attttgcaat aatggaacct aacgctgtcg gtgccatcat acagccggca ataaacgcct
    16681 gataaccaaa tcctacctgt aacatcaatg gcataaggaa cggtacacag ccggtcccca
    16741 gacgggttgc aatattgcct acgataccga tcgagaaagt gcgggtttta aataaatcta
    16801 atgaaattaa tgggtttggc gtgcgtcgtg catggagaat atagagaagc agtaacccga
    16861 tgctggtgac aattaccgtc aaggcaatcc agctggcgac aatcttttcc ccgaatagct
    16921 ctattccgct tgagaagaga acaaggctga ggccaaacag caaaaagcca gtgatatcga
    16981 atctgcgtcg tgcggtggtg aaattgggca tatgtttgcg cgcgtaaaga aggcccgcaa
    17041 tacctatggg gatattgatt aaaaatatcc agtgccaggt tgcccaggtg accagcacgc
    17101 cgccaagaac ggggcctaaa attggcccca ccagacccgg catggcgaca aaattcaata
    17161 ctggaagaag ttcattacga ggataagcgc gcagtaaggc cagccgagca acaggcatca
    17221 tcattgcgcc gcctatcccc tgaataaccc ggaagacaac cagctgtggt agcgagttgg
    17281 aaagtgcgca ggccagagaa cccaacgtga acagactcac ggcaagggta aaaatgcgac
    17341 gcgtaccgaa gcgatcggct agccatccgc ttaccggaat aagcatcgcc accgtcagcg
    17401 tataactgat gatggctgat tgcatcgcga gaggagaacg attaaggcta tgagcgattg
    17461 cgggtaaggc ggtattcaga atagtggcat caagtgcctg catgaagaag gccatcgccg
    17521 cgatccacgg caaacccgcc atactgcgct tctttttatc gctcattcaa tgtcctgtta
    17581 tcgggttatc acttatcagg tgagcgtagc agcgcctgac aagctttaaa tgccgcgtcg
    17641 ccatcgcttt ggataatcgc atcgacaatc gcctgatgca gatccagctt tatcactgtg
    17701 tcgctggtaa ttgacgtgaa gtaagtgtga taaaccgaat ggaatagcga ggcgaatgag
    17761 gtcaaaaacg gattggcgct catttcatag atatgctcat gccaggccat atcgacttcg
    17821 atccagcgtt cacggcgaaa gttctctttt aatgccgcca tttcggccat taacgtattg
    17881 agatgcgctt tctgttctgc ggtgccaacc gttgctgcca gtaggcaggc ttgcggctcc
    17941 agacagatac gcataaccag aaagtgatcg atgacctgat gaaagttctc ttctgtcatc
    18001 caccaggtaa gcaattcctg atcaagaaaa ttccagttcg attgtggcat tacccgggta
    18061 ccaattcgcg gtcgcggtaa aaccatccct tttgccgtta acgttttgac cgcttcgcgt
    18121 accgctgtac gactcactcc aaattgctcg cccagctcaa tctcaccggg caaaatggtg
    18181 ccgggttcat attcaccttt taagatccgt tgcgccagct tctcagccag aacatacgaa
    18241 aggtttttct gtgcagctaa ctgttgtgcg cttaaaggca ttactaatct tcctttttct
    18301 ttttattcct ccttagtatg ccaccaggaa gtgtgattac ggttgcaaaa acggcaaatt
    18361 gcttgtttta tggcacatta acggggcttt tgctgaaaaa atgcgcggtc
//
LOCUS       DAZGBC010000094        18289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGBC010000094 DAZGBC010000000
VERSION     DAZGBC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.094"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(112..759)
                     /gene="ychE"
                     /locus_tag="ACHK1J_003139"
     CDS             complement(112..759)
                     /gene="ychE"
                     /locus_tag="ACHK1J_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO7912378.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSFQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(790..843)
                     /gene="ychE"
                     /locus_tag="ACHK1J_003140"
     CDS             complement(790..843)
                     /gene="ychE"
                     /locus_tag="ACHK1J_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO7912379.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1236..3911
                     /gene="adhE"
                     /locus_tag="ACHK1J_003141"
                     /gene_synonym="adhC"
     CDS             1236..3911
                     /gene="adhE"
                     /locus_tag="ACHK1J_003141"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO7912380.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4213..4830)
                     /gene="tdk"
                     /locus_tag="ACHK1J_003142"
     CDS             complement(4213..4830)
                     /gene="tdk"
                     /locus_tag="ACHK1J_003142"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO7912381.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5434..5847
                     /gene="hns"
                     /locus_tag="ACHK1J_003143"
     CDS             5434..5847
                     /gene="hns"
                     /locus_tag="ACHK1J_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO7912382.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(5991..6899)
                     /gene="galU"
                     /locus_tag="ACHK1J_003144"
     CDS             complement(5991..6899)
                     /gene="galU"
                     /locus_tag="ACHK1J_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO7912383.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7101..8114)
                     /gene="rssB"
                     /locus_tag="ACHK1J_003145"
     CDS             complement(7101..8114)
                     /gene="rssB"
                     /locus_tag="ACHK1J_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO7912384.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8206..9111)
                     /gene="rssA"
                     /locus_tag="ACHK1J_003146"
     CDS             complement(8206..9111)
                     /gene="rssA"
                     /locus_tag="ACHK1J_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO7912385.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9224..9682
                     /gene="ychJ"
                     /locus_tag="ACHK1J_003147"
     CDS             9224..9682
                     /gene="ychJ"
                     /locus_tag="ACHK1J_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO7912386.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAVLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9732..10574
                     /gene="purU"
                     /locus_tag="ACHK1J_003148"
     CDS             9732..10574
                     /gene="purU"
                     /locus_tag="ACHK1J_003148"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO7912387.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10734..10818
                     /locus_tag="ACHK1J_003149"
     tRNA            10734..10818
                     /locus_tag="ACHK1J_003149"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10768..10770,aa:Tyr,seq:gta)
     gene            10862..10993
                     /locus_tag="ACHK1J_003150"
     ncRNA           10862..10993
                     /ncRNA_class="other"
                     /locus_tag="ACHK1J_003150"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11028..11112
                     /locus_tag="ACHK1J_003151"
     tRNA            11028..11112
                     /locus_tag="ACHK1J_003151"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11062..11064,aa:Tyr,seq:gta)
     gene            11159..11290
                     /locus_tag="ACHK1J_003152"
     ncRNA           11159..11290
                     /ncRNA_class="other"
                     /locus_tag="ACHK1J_003152"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11474..12151)
                     /gene="narI"
                     /locus_tag="ACHK1J_003153"
     CDS             complement(11474..12151)
                     /gene="narI"
                     /locus_tag="ACHK1J_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7912388.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12151..12861)
                     /gene="narJ"
                     /locus_tag="ACHK1J_003154"
     CDS             complement(12151..12861)
                     /gene="narJ"
                     /locus_tag="ACHK1J_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7912389.1"
                     /translation="MIELVIVSRLLEYPDAALWQYQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LMKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12858..14396)
                     /gene="narH"
                     /locus_tag="ACHK1J_003155"
     CDS             complement(12858..14396)
                     /gene="narH"
                     /locus_tag="ACHK1J_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7912390.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMV
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14393..18136)
                     /gene="narG"
                     /locus_tag="ACHK1J_003156"
     CDS             complement(14393..18136)
                     /gene="narG"
                     /locus_tag="ACHK1J_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO7912391.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4609 a         4792 c         4378 g         4510 t
ORIGIN      
        1 tgagaagaat atttttacct attccttttg cttttgataa ctgcgcggga acaaagtaaa
       61 aataatgacg aagtaaacgg agcatggcag ctccgtttca tttgaaagga attaattaag
      121 caggccgggg aaaatcccct taataccagt aacgataaat tcaatcccca atgccatcag
      181 caatagcccc ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag
      241 ccacggtgcc atgcggaaca atccccaaca acataaagcg aacaatgcaa tagccacaaa
      301 gaaaccaaac agatagctaa tgctgtgata acgcgtaccc cagacgatgg tagaactgat
      361 cgcccctggc cccgccatca acggcaacgc cagtggcacc acaccaatgc tttcacgtac
      421 cgcggtttct gatttttctt gcttgttctg tttatcctcg ccaagcttgc cgctgatcat
      481 cgacatcgct attgtcacca ccaggatacc cccggcgata cggaacgaat caattgatat
      541 accaaaaagt tgtagaatcg tgtcgccgag aaaaagcgag atccacaaga taatggccac
      601 agacaggttg gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgaa aactggtcat
      661 gctgataaag acgggaataa tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa
      721 tttgaagtaa acgggaaaat caaaaagggt ctgaatcacg gttagctccg aagcaaaagc
      781 cggataatgt tagccataaa taaggttgaa aagacgcgct gacaatacgc cttttgacag
      841 catttttcac ctcctaacta cttaaaattg ctatcattcg ttattgttat ctagttgtgc
      901 aaaacatgct aatgtagcca ccaaatcata ctacaattta ttaactgtta gctataatgg
      961 cgaaaagcga tgctgaaagg tgtcagcttt gcaaaaattt gatttggatc acgtaatcag
     1021 tacccagaag tgagtaatct tgcttacgcc acctggaagt gacgcattag agataataac
     1081 tctgatgttt aaactctttt agtaaatcac agtgagtgtg agcgcgagta agcttttgat
     1141 tttcatcggt taagcaaatc atcaccgcac tgactatact ctcgtattcg agcagatgat
     1201 ttactaaaaa agtttaacat tatcaggaga gcattatggc tgttactaat gtcgctgaac
     1261 ttaacgcact cgtagagcgt gtaaaaaaag cccagcgtga atatgccagt ttcactcaag
     1321 agcaagtaga caaaatcttc cgcgccgccg ctctggctgc tgcagatgct cgaatcccac
     1381 tcgcgaaaat ggccgttgcc gaatccggca tgggtatcgt cgaagataaa gtgatcaaaa
     1441 accactttgc ttctgaatat atctacaacg cctataaaga tgaaaaaacc tgtggtgttc
     1501 tgtctgaaga cgacactttt ggtaccatca ctatcgctga accaatcggt attatttgcg
     1561 gtatcgttcc gaccactaac ccgacttcaa ctgctatctt caaatcgctg atcagtctga
     1621 agacccgtaa cgccattatc ttctccccgc acccgcgtgc aaaagatgcc accaacaaag
     1681 cggctgatat cgttctgcag gctgctatcg ctgccggtgc tccgaaagat ctgatcggct
     1741 ggatcgatca accttctgtt gaactgtcta acgcactgat gcaccaccca gacatcaacc
     1801 tgatcctcgc gactggtggt ccgggcatgg ttaaagccgc atacagctcc ggtaaaccag
     1861 ctatcggcgt aggcgcgggt aacactccag ttgttattga tgaaactgct gatatcaaac
     1921 gtgcagttgc atctgtactg atgtccaaaa ccttcgacaa cggcgtaatc tgtgcttctg
     1981 aacagtctgt tgttgttgtt gactctgttt atgacgcagt acgtgaacgt ttcgcaaccc
     2041 acggcggcta tctgttgcag ggtaaagagc tgaaagctgt tcaggacgtt atcctgaaaa
     2101 acggtgcgct gaacgcggct atcgttggtc agccagccta taaaattgct gaactggcag
     2161 gcttctctgt accagaaaac accaagattc tgatcggtga agtgaccgtt gttgatgaaa
     2221 gcgaaccgtt cgcacatgaa aaactgtccc cgactctggc aatgtaccgc gctaaagatt
     2281 tcgaagacgc ggtagaaaaa gcagagaaac tggttgctat gggtggtatc ggtcatacct
     2341 cttgcctgta cactgaccag gataaccaac cggctcgcgt ttcttacttc ggtcagaaaa
     2401 tgaaaacggc tcgtatcctg attaacaccc cggcttctca gggtggtatc ggtgacctgt
     2461 ataacttcaa actcgcacct tccctgactc tgggttgtgg ttcctggggt ggtaactcca
     2521 tctctgaaaa cgttggtccg aaacacctga tcaacaagaa aaccgttgct aagcgagctg
     2581 aaaacatgtt gtggcacaaa cttccgaaat ctatctactt ccgccgtggc tccctgccaa
     2641 tcgcgctgga tgaagtgatt actgatggcc acaaacgtgc gctcatcgtg actgaccgct
     2701 tcctgtttaa caatggttat gctgatcaga tcacttccgt actgaaagca gcaggcgttg
     2761 aaactgaagt cttcttcgaa gtagaagctg acccgaccct gagcatcgtt cgtaaaggtg
     2821 cagaactggc aaactccttc aaaccagacg tgattatcgc gctgggtggt ggttccccga
     2881 tggacgccgc gaagatcatg tgggttatgt acgaacatcc ggaaactcac ttcgaagagc
     2941 tggcgctgcg ctttatggat atccgtaaac gtatctacaa gttcccgaaa atgggcgtga
     3001 aagcgaaaat gatcgctgtc accaccactt ctggtacagg ttctgaagtc actccgtttg
     3061 cggttgtaac tgacgacgct actggtcaga aatatccgct ggcagactat gcgctgaccc
     3121 cggatatggc gattgtcgac gccaacctgg ttatggacat gccgaagtcc ctgtgtgctt
     3181 tcggtggtct ggacgcagta actcacgcca tggaagctta tgtttctgta ctggcatctg
     3241 agttctctga tggtcaggct ctgcaggcac tgaaactgct gaaagaatat ctgccagcgt
     3301 cctaccacga agggtctaaa aatccggtag cgcgtgaacg tgttcacagt gcagcgacta
     3361 tcgcgggtat cgcgtttgcg aacgccttcc tgggtgtatg tcactcaatg gcgcacaaac
     3421 tgggttccca gttccatatt ccgcacggtc tggcaaacgc cctgctgatt tgtaacgtta
     3481 ttcgctacaa tgcgaacgac aacccgacca agcagactgc attcagccag tatgaccgtc
     3541 cgcaggctcg ccgtcgttat gctgaaattg ccgaccacct gggtctgagc gcaccgggcg
     3601 accgtactgc tgctaagatc gagaaactgc tggcatggct ggaaacgctg aaggctgaac
     3661 tgggtattcc gaaatctatc cgtgaagctg gcgttcagga agcagacttc ctggcgaacg
     3721 tggataaact gtctgaagat gcattcgatg accagtgcac cggcgctaac ccgcgttacc
     3781 cgctgatctc cgagctgaaa cagattctgc tggataccta ctacggtcgt gattatgtag
     3841 aaggtgaaac tgcagcgaaa aaagaagccg ctccggctaa agctgagaaa aaagcgaaaa
     3901 aatccgctta atcagtagcg ctgtctggca acataaacgg ccccttctgg ggccgttttt
     3961 ttgtttaccc aaagcaactt ttccataaac cgacagcatt agccttcatc atatttgcga
     4021 cgatgtataa cgcctaaaca cagcgatatt gtactttaca gatcacaagt caacgtcggt
     4081 gcttaagagc cctgtgaggc gtatagcggc gttaaaaaac tgccgagaag ggtgtatagc
     4141 ccggaagaag tgcgtaaaac gaactgacag gataaaagtg ccctgctcac cctgtcagta
     4201 aagaaattct tattaatcgt ggcgatgcct ttcctgaata gccgttaatg agccgacttg
     4261 taacgcctct ttatagtgtt tacggcatac agaaacgtat cgttcattac caccaataac
     4321 cacctgctca ccttcgttat aaggtctgcc tgcttgatca agacgcagca ccatgcttgc
     4381 cttacggcca caaaaacaga tggtttttaa ttcaaccagt ttgtcggacc atgccagtaa
     4441 gtattggctg ccaataaata attcacctcg aaaatcggta cgtaaaccat aacaaagtac
     4501 gggtatatcg agttgatcga caacctccga taattcatat acttgttgtc tggttaaaaa
     4561 ctggcattca tcaaccagta cgcaatgaat tgcctgctgt tcatgttccg cacgaatctc
     4621 atcaaataat gatgaatttt ggttaaataa ttttgcaggc gatgacaaac ctatacgcga
     4681 actgactttc ccggcaccaa agcgatcatc aatttctgcc gtatatacga cagtgcgcat
     4741 gccgcgttcc tggtaattgt atgaagattg caacaatgct gtagacttac ccgcattcat
     4801 tgcggaatag tagaaatata gctgtgccat cagccacagg ccctcaatga tatgaaataa
     4861 agttggctgg agtttatcat aattcgtcag tttttcagct caacagtatg cacagaagtg
     4921 gtatatgcgt tctcccttac gaagccttgc ataatccttc tgagctatca ttacaactgc
     4981 ctcgcttgtt ataagcgggt aaatgactgc tggtaactat tcacaatctt taacctgttg
     5041 cgcaagtaat agccctctgt tgacctccag gagatagtgc aatactaagt ccctgctctt
     5101 attgcgactg ttctactttt catcattcgc ttaataggga attctcgtaa acacgactaa
     5161 tacagaagac tgaaaggtcg tcagcttacg ataatctccc cataaaatgt gacatgaatc
     5221 aggaagtttt aacctcacgt gctgcgaaat catcggtaca aatagggcta tatgccgcgt
     5281 cttttctggc taattttatg aaaagatatt tattggcggc acaaaataaa gaacaatttt
     5341 gaattactta cattcctggc tattgcacaa ctgaatttaa ggctctatta ttacctcaac
     5401 aaaccacccc aatataagtt tgagattact acaatgagcg aagcacttaa aattctgaac
     5461 aacatccgta ctcttcgtgc gcaggcaaga gaatgtacac ttgaaacgct ggaagaaatg
     5521 ctggaaaaat tagaagttgt tgttaacgaa cgtcgcgaag aagaaagcgc ggctgctgct
     5581 gaagttgaag agcgcactcg taaactgcag caatatcgcg aaatgctgat cgctgacggt
     5641 attgacccga acgagctgct gaatagcctt gccgccgtta aatctggcac caaagctaaa
     5701 cgtgctcagc gtccggcaaa atatagctac gttgacgaaa acggcgaaac taaaacctgg
     5761 actggccagg gccgtactcc agctgtaatc aaaaaagcaa tggatgagca aggtaaatcc
     5821 ctcgacgatt tcctgatcaa gcaataatct tttgtagatt gcacttgctt aaaatcccgc
     5881 catcggcggg attttttatt gtccggttta agacaattta ataagaataa gctataaaaa
     5941 aacggcgtcg attgctcaac gccgtttcgt ggataacacc gatacggatg ttacttctta
     6001 atgcccatct cttcttcaag ccaggcttta aattccgtgc caagggtgtt atgacgaata
     6061 ccgtattcaa cgaaggcctg catgtaacct aatttattac cgcagtcatg gctcttccct
     6121 ttcatatgat aggcttcaac cgtttctttt tcgattagca tatcaattgc gtcggtgagc
     6181 tgaatttcat caccagctcc cggaggggtt tttgccagca acggccaaat atccgcacta
     6241 agtacgtaac gacccacaat agcgagatta gacggcgcaa catccgcttt tggtttttca
     6301 accacgccaa ccatcggtac gctttcaccc ggcgctaatt caacgccttt gcaatccaca
     6361 acgccatatg cggtcacatc agcaaccggt tcaaccatga tctggctatg acccgtttca
     6421 tcgaagcggc ggatcatctc tgccaggtta tcctgtgaca aatcggattc atattcatcc
     6481 agaataacgt caggcaaaat aacagccaca ggttcatcac ccactaccgg gtgagcacac
     6541 aataccgcgt gtcccaggcc tttcgccagc ccctgacgaa cttgcataat agttacgtgc
     6601 ggcggacaaa tagactgcac ttcatcaagc agttgacgtt ttacacgttt ttccagcatt
     6661 gcttccagtt caaaactggt atcaaagtgg ttttcaatag agtttttaga tgagtgtgta
     6721 accagcacaa tttcagtaat gccagccgca atacattcat tcacgacgta ttgaattaat
     6781 ggcttatcga caagtggcag catctctttc gggatggctt tcgtcgccgg caacatcctg
     6841 gttcctaatc ccgcaacggg gataacggct tttttgactt tcgtattaat ggcagccatt
     6901 taaattctcc tggactgttc gtgttttgaa cgtgttcata tttctgtatc gcatcccagt
     6961 atatcagcac cccggagggg ctaagttctg aaaaggatgc gagttagcat aattaagaca
     7021 attacttata cgaatggcga cgatagtacc accagccgac attagctggt aatgcaaatt
     7081 tagcccgcgt catcgtttgc tcattctgca gacaacatca agcgcagtcg accaccggtt
     7141 ccccatattt ggcattgcca ggcatcgcat cgctggctca attgattcaa ataagcgtta
     7201 cctaaagtgc ctaacggaac accattactg atttgcacct ggtgttcgcc ggtatttaac
     7261 gtcgcattca gacccgcaga aaccagaatg agatttttca gttcgcgatg ataatagcca
     7321 actaatagcg gaaactgccc cggcagattg gcctgacgga gtaaatggtt tacctgcttc
     7381 aataacgcgc ccaactctgg caaccgttga ttttggtgtg caagctgttc ctgtaataat
     7441 ccgttaaaca atgcgcgtaa taacaaggca gcaagtacgc cattatgtcc agctcgggtt
     7501 acatcaaggc aataaaatgc cagatcgttt tccgaaagtg cggcaatatc aagcaccagg
     7561 ccgggtttgt ccgcggcaac caattgacgg taattaaccc ggcaatggga aatcacctgc
     7621 tgaaccggcg gttgtagttc ctgtaataat ttcgccgctg cggcagggtt atcaaccatt
     7681 gcatcccagt cgcgaaaaag cctttcctct tcctcaacgc gcgaattaaa catgctggga
     7741 tagagacagg caaaaaccat ctcgcgcaag cgattcagat ctttaactgg tttcagcaaa
     7801 acatcttcaa cgcccagacg taacgctttg gcaatatctg ccatattttc agtggcagat
     7861 atcaccagaa ctggggtctg gtcgcctctg ttacgtatat gctccagcag tttaagcccg
     7921 ttcattcgtg gcatcgcgat atcacatatc atcaggtctg gagtgaaacc tcccagcaac
     7981 tcaagggcat ccaccccatc agccgccagt accgttgtcg ctcccaatga ggaaaaccac
     8041 gaatccagaa gcgagcgaaa tacctgctca tcttcaacaa tgagaatctg ttttccgacc
     8101 aatggctgcg tcatgttctc tcccctgact ggctttactc aatagtggca tgctattgcc
     8161 acctgcgcct gtcagaatta acttaagtgt agataaaaat tctggtcaaa tgttggtgcg
     8221 taccaacggc aaaagttcgt ccattttctt ttccactgcc agctgcccgg ccgcaatggc
     8281 agcgtgcgcg cgatggaaat caagcgtaga tatttgcggg caaacaggtt gaatcagaat
     8341 atcgggcgga tcacctgcca tgcggttcct tttaaggcgg ttctccagca cctggataga
     8401 agtggtcata atctctgttg ccgttggtgc tgtcaccgcc cgacgtgtcg ttatgctgcc
     8461 taacctttct ttcagacgcg catgccacgg cagagaatca ccattctcgc tattttcttc
     8521 actgacatta aaggagagca aatcttgttg catcaaatga gcatcgtgct gcaggtcgac
     8581 cgctatcaca atatcagcac ccaatgcacg cgtgagcgaa ataggaattg ggttaacgac
     8641 tgccccatca accagccagt agccgttatg cgcaacgggt gccattagtc ctggaatact
     8701 gcatgatgcg cgaatagcaa gatggagatc gccttcagta aaccataatt cacgcccagt
     8761 acttaaattg gtggcaacag ccgcgaagcg acgggaacaa ttttcgatct ctgtttccgg
     8821 cattatttcg cggtattgat tgaagacacg ctcgccgcgc agtaacccac cgcgctgcca
     8881 ggagagatcc atcaggcgta aaacatccca ataactgaaa gaggttaccc aatcttccag
     8941 cgcagataat cgatcgcatg cataggccgc gcccaccagc gaaccaatag aacatcctgc
     9001 aacgatatca atttcaatac ccactttttt tagcgcatta ataacgccaa tatgcgacca
     9061 acctctcgcc gcgccagatc ccagcgccag ccctattttt atctttctca ttattcccgc
     9121 aagattcccc tggaatgcaa tcgtagccac atcgctactg ctcggttaac atagtgctac
     9181 tcaggcggtt aatacgccgt tattgtttcc agggagatta tttgtgtctc agctttgtcc
     9241 ctgtggtagt gctgtcgagt atagcctatg ttgccaccct tatgtgtctg gtgaaaaggt
     9301 tgcacctgat ccagaacatc tcatgcgttc gcgttactgc gcttttgtga tgcaagacgc
     9361 agattattta ataaagacct ggcatccatc ttgtggggca gcggtgttac gtgccgaatt
     9421 gattgccgga tttgcgcata ctgaatggtt gggattgacc gtatttgaac attgttggca
     9481 ggacgtcgac aatatcggat ttgtcagttt tgttgctcga tttaccgaag gtggcaaaac
     9541 aggtgcaata attgaacgtt cgcgattttt gaaagaaaac ggtcaatggt actatattga
     9601 cggtacacgt ccgcagtttg gccgcaacga tccctgccct tgtggttcag gtaaaaaatt
     9661 taaaaagtgc tgcggccaat aatggttgac ggtacggttt agcaaacact ctcaacaagg
     9721 tttttccagc aatgcattca ctccaacgta aagttctgcg tactatttgt ccggaccaaa
     9781 aaggtctgat cgcacgtatt accaatattt gctacaagca cgagttaaat atcgtacaga
     9841 acaatgaatt tgttgatcac cgtaccgggc gcttttttat gcgcacggaa ctggaaggga
     9901 tttttaatga ttccaccctg ctggcggatc tcgatagcgc attgccagaa ggctccgtgc
     9961 gtgagctgaa tcctgccggt cgtcgccgga tagtgattct ggtcactaaa gaagcgcatt
    10021 gccttggcga tttgttgatg aaagccaatt acggcggcct ggatgtcgaa atcgcggcag
    10081 ttattggtaa ccacgatact ttacgttctc tggttgagcg ttttgatata ccgtttgagc
    10141 tggtaagcca tgaagggtta agccgcaacg agcacgatca aaagatggcg gatgccattg
    10201 atgcttatca acctgactac gtggtgctgg cgaagtatat gcgggtatta acaccggaat
    10261 ttgtgtcacg cttcccgaat aagatcatca atattcacca ttccttcctg ccagcgttta
    10321 tcggcgcacg tccttatcac caggcctatg aacgtggcgt gaagattatt ggtgcaaccg
    10381 ctcactatgt gaatgacaat ctggacgaag gcccaatcat catgcaggac gttattcatg
    10441 tcgatcatac ctacacagct gaagatatga tgcgcgcagg tcgtgacgtc gagaaaaacg
    10501 tcttaagtcg tgcgctttac aaagtactgg cgcagcgcgt ctttgtttac ggtaatcgga
    10561 cgattattct ttaatcgcca gcaaaaataa ctggttacct ttaatccgtt acggataaaa
    10621 attacgcaac cagttcattt ttctcaacgt aacactttac agcggcgcgt catttgatat
    10681 gatgcgcccc gcttcccgat aagggagcag gccagtaaaa agcattaccc cgtggtgggg
    10741 ttcccgagcg gccaaaggga gcagactgta aatctgccgt catcgacttc gaaggttcga
    10801 atccttcccc caccaccatg attcaccaca gcgatgtggt aacttcggtg agattgtgaa
    10861 agtttgcact catcggggaa gggtgagaac cttcgactaa ggttcgactc gagcgaaagc
    10921 gagagaacat tgccacaggc aacggcccga agggtgagga gcgaagcgac gaataatcct
    10981 tcccccacca gcacctcaag cattacctca aaatttgaat tacctctggt ggggttcccg
    11041 agcggccaaa gggagcagac tgtaaatctg ccgtcatcga cttcgaaggt tcgaatcctt
    11101 cccccaccac catcactttc aaaagtccct gaacttccca acgaatccgc aattaaatat
    11161 tctgcccatg cggggaagga tgagaagctt cgaccaaggt tcgactcgag cgccaatgag
    11221 aaagcgttgc cgcaggcaac tacccgaagg gcgaagcgcg cagcgctgag taatccttcc
    11281 cccaccacca tttcttcctg aaatactcaa aacctgttac tccgagctaa ttctgcccga
    11341 tggcaccgcg tttaccggac ctacaatatc ctcccctgta ggtcggataa gcgaagcgca
    11401 tccgacaact caccccgcac cgtgcccata aaaaaacccc gccgaagcag ggtctatgtg
    11461 aactaaaatt cgcttagtga cgagcgcgca ccagctggta cttacgtgtc agatactcca
    11521 ccggtacgct ccagatgtgt accagacgcg agaacgggaa cagcaggaat aacgtcatcc
    11581 ccagtaccag gtgtagacgg aagataaacg ccacaccatc gaggtgttga gaagcgccac
    11641 cgtggaaggt caccaccgac tgcgcccagc caaccagttt catcatctcg ctaccgtcca
    11701 tatgttgggc ggagaacgga atggtcaaca ggcccagcgc gcactgaata acgagcagcg
    11761 acaggatcag gatatccgct ccggtagtgg ttgcacgcac gcgtgggctg aacagacgac
    11821 gcttaagcaa cagcacgccg ccaatcagac acagcacgcc gctggcacca ccagcaaaca
    11881 tcgccatttt ctgtttcact tcaatcggca gccaggcttc atacatccag tgcggcgtca
    11941 acataccgaa gaagtgaccg acaaaaatcc ccagaatacc gatatggaac aggttcgacg
    12001 ccaggttcat ccctttgcga tccagcatct ggctggacgc cgcgcgccag gtgtactgcc
    12061 cgtagtcata acgcagccag ctaccaatca ggaagaccgc cccggctatg tacgggtaga
    12121 tatcaaagaa gaacatattc aggaattgca ttagtgctgt cctccggtgg tgatattcag
    12181 atattgcggc gcgacggcac cggcaaagcg acgctgatga gcagtgattg ctgaatcacc
    12241 gcagcctttg tcagcaaaga atttaacctg ctcttcttcc cagacagcat ccagcgcctg
    12301 cggcgtatcg tcgcgcgctt cgtcggcaat tttttccgcc actttgtcgc tgtcgatagc
    12361 ggtattcgcc agtttcatca gcagatcaaa cagcacggca taacggcttt cacgctgttg
    12421 cagacgcgcg ctcagcaatg ccagaatcgg cgcgatatct ttcaaacctt ccacggcttc
    12481 gctttgcggt agctgcgcca ggtactccag atacagcggc agatggtccg gcaattcgcg
    12541 gctgtttaac tgcaagccgt gctgctcgta ctgcgccagc aggtccacca tcgcctggcc
    12601 gcggtcgcgg gattcgccgt gcacatgttc aaacagcaac agtgacgtgg cgcggccacg
    12661 gtcgaacagt tcgctgtact gcgcctgggc atcgagcgga tccatcgtcg ttaaatcgcg
    12721 caggaaaatg cccagcgcat gggcatcctc ttttggcaga tttttcgacg cggcaatcgc
    12781 ctcaaacatc tcttgttgat actgccataa ggcagcatcc ggatattcaa ggagacgcga
    12841 tacaatcacg agttcgatca tgggtgcggc tccgttttgc tggtcacatc gatggcatcg
    12901 atacgacggc tgttgaacag attgaatttg gtatctgaac cgtggcagcc atcaccaaag
    12961 gtaaagccgc agccattttt ctccgggaag gcttcccgtg ccagttcacg atgactactc
    13021 ggcaccacaa agcgatcttc gtagttagca atcgccagat aacggtacat ctcctgtgcc
    13081 tgggcttcgg tcagaccgac ctcttccagc gcacgggtat ccactttccc gtcaacggtt
    13141 tcagcacgtt tgtaatgacg catcgccagc atacgtttca gcgcgcgcag taccggtttg
    13201 gtatcaccgg cggtcagcag attcgccaga tactgtaccg gaatacgtag gctttcgacg
    13261 tctggcagaa tgccgttgct acccagctca cccgcgtctg ctgcagactg aatcggagac
    13321 agaggcggca cgtaccagac catcggcagt gtgcgatatt ctggatgcag cggcagcgcc
    13381 agtttccatt ccattaccat tttataaacc ggcgactgct gtgcagcttc aataacgctc
    13441 agcggaatac cgtctttaat cgcctgctcg atgactttcg gatcgttcgg atcgaggaac
    13501 acctccagct gacgctggta gagatctttc tcgttctcgg tgctggctgc acgttcaatc
    13561 gcgtcggcat cgtacagcag cacgccaaga taacggatac gaccgacaca ggtttctgag
    13621 cacacggtcg gctgacccgc ttcaatacgc ggatagcaga agatgcactt ctcagactta
    13681 ccgctcttcc agttgaagta gatttttttg tatgggcatc cggtgatgca catacgccag
    13741 ccacggcatt tatcctggtc gatcaggacg atgccatctt cttcacgctt gtaaatcgca
    13801 ccgctcgggc aggtcgccac acatgccggg ttcaggcagt gttcgcacag gcgcggcaaa
    13861 tacatcatga aggtgttttc gaactggcta tacatcgcct tctggatgtt gtcgaagttc
    13921 ttgtctttcg ccagtttgtc aaactcaccg cccaggtcat cttcccagtt cggccctttt
    13981 tcgattttcg ccatccgttc cccggtaatc agcgaacgcg gacgggcaat cggctgcgat
    14041 ttgctgcctt ccggcgcggt atgcaggttc tgatagtcaa aatcgaacgg ctcgtaataa
    14101 tcgtcgatcc ccggcagatg cgggttagcg aagattttac ccagcagcat ggcacggtta
    14161 cccatgcgcg gctgcagttt gccgttgatt ttacggatcc agccgccttt gtatttttcc
    14221 tggttttccc agtcagtcgg gaagccctgg cccggcttgg tttccacgtt gttgaaccac
    14281 gcgtattcca cgccttcacg gctggtccag acgtttttac aggtaactga acaggtgtgg
    14341 cagccgatgc acttatcgag attcagcacc atgccgactt gtgaacgaat tttcatttta
    14401 cgctctcctg tacctggtca ttgccttcgc catctaacca gtcaatgttc ttcatcttac
    14461 gcactacaac aaactcatcg cggttagaac ctacggtgcc gtagtagtta aagccgtatg
    14521 ccagatgggc atagccgccg atcatatgcg tcggtttcgg cgtgatacgg gtgaccgagt
    14581 tatggatacc accacgctgt tgggtaattt ccgaaccagg caggttaacg atacgttcct
    14641 gcgcgtggta catcatggtc atccctgccg gaacacgctg gctgacaacc gcacgggcag
    14701 tcagagcacc gttgctgttg aatacttcaa tccagtcgtt atcggcgata cccagatctt
    14761 tggcatcggc ttcactcaac cagaccaccg gaccaccgcg acctaaagtc agcatcagca
    14821 ggttgtcgct gtaggtggag tggatacccc acttctggtg cggcgtcagg aagttgagcg
    14881 ctttttccgg gttgccgttg gatttctggc ctatcacttc tttcaccgaa cgggtgtcga
    14941 tcggcggacg ataaaccagc aggctttcac cgaaatcacg catccactgg tgatcctgat
    15001 acagttgctg acgaccagag agcgtacgcc atgggatcag ctcgtgaacg ttggtgtaac
    15061 cggcgttgta agaaacgtgt tcatcttcca gaccagacca ggtcgggctg gagataattt
    15121 tgcgcggctg tgcctgaata tcgcggaagc ggatcttctc gtcttcttta ttcagcgcca
    15181 gatgcgtatg gtcacgaccg gtaaactcgc tcagggcagc ccaggctttc acggctacct
    15241 gaccgttggt ttccggtgcc agcgtcagga tcatctctgc cgcatcaatt gcggtgttca
    15301 gcatcggctg gcctttcgcc ggaccttccg ctttggtgta gttgagctta cgcagcagat
    15361 ccatctcgct ctgggtgttc caggcaatcc ctttaccgcc gttaccgatt ttctccatca
    15421 gcgggccgat agaggtaaag cgttcgtaag ttgccggata atcgcgctct acgaccataa
    15481 tgtgcggtgc agttttgcct gggatcaggt cgcattcgcc ttttttccag tctttcacat
    15541 ccagcggctg cgccagttca gcggcagagt cgtgctggat aggcagcgtg acgatgtcgg
    15601 tttctttacc cagatggccg acgcacactt cggagaattt cttcgcgatg gctttgtaga
    15661 tttcccagtc gcttttcgct tcccaggccg gatcgaccgc cgcagacagc gggtgaataa
    15721 acggatgcat atccgaagta ttcatgtcgt ctttttcgta ccaggtcgcc gtcggcaaaa
    15781 tgatgtcgga atagagacag gtgctcgaca gacggaagtc cagcgtaacc accagatcca
    15841 gcttgccttc cagaccattg tcctgccagt ccacttcttc cggcttcacg ccgccctgct
    15901 gccccagatc tttaccctgg ataccgtgct ccgtccccag caggtacttg agcataaact
    15961 catgaccttt accggaagaa ccgagcaggt tagaacgcca gatgaacagg ttgcgcgggt
    16021 ggtttttacc gttttctggt tgttctgccg caaaacggat ggaaccctct ttcagggatt
    16081 tcaccgtata gtccaccgga ttcatcccgg ctttttccgc ttcgccagcg atagtcagcg
    16141 ggttagtgcc taactgcggt gcagacggca gccagcccat gcgttccgca cggacgttaa
    16201 agtcgatcag gtgtccggta taacgggatt tatccgccat cggtgacagc aactcttccg
    16261 ccgtgacggt ttcataacgc cactggctgg agtggttata gaaataagaa gtgctgttca
    16321 tgtgacgcgc cggacgctgc cagtcaaggg caaacgccag cggctgccag ccggtttgcg
    16381 gacgcagttt ttcctgacct acatagtgcg cccagccgcc cccgctctga ccgacacagc
    16441 cgcagaaaat cagcatgttg atcagaccac gatagttcat atcgaggtga taccagtggt
    16501 tcagccccgc accgacgata atcatcgaac gaccgtgcgt tttatcagcg ttatcggcaa
    16561 attcacgggc gatgcgaata atctggctgc gagaaacgcc ggtaatctgc tcggcccagg
    16621 ccggggtata agctttcaca tcgtcatagc tggttgcaca gttaacgtcg ttcaggccac
    16681 gttccagacc gtagtttgcc agcgtcaaat cataaacggt ggtcaccagg gcggtgctgc
    16741 catcagccag ttgcaggcgt ttcaccggca gtttgtgcag cagcacgttt tccagttcca
    16801 ctttgttgaa gtgttccgtg ccgtcgccac caaagtacgg gaagcctacc tcagcgatct
    16861 catcctgact acccagcagg ctcagttgca gctcggtttc ttcgccagtt ttgccgtcgc
    16921 gctgctcaag attccatttg cccttctcgc cccagcggaa gccaatagaa ccgttcggcg
    16981 caaccatttc gccattggta ttaaaggcga cagttttcca ttccggattg ttttcctggc
    17041 ccagcgcatc aaccagatca gcagcgcgca gcatacgacc tgcagcgtaa taaccgtcgc
    17101 gttcttccag catcaccagc atcggcatgt cggtgtagcg acgcacatag tcggtgaaat
    17161 actggcttgg gttgtcgagg tggaattcac gcagcattac gtggcccatc gccagcgcca
    17221 ttgccgcatc ggtgccctgt ttcggtgcca gccacagatc gcacagtttg gcgatttcag
    17281 cgtagtctgg tgtgacggca acagttttgg tgcctttgta acgcacttca gtaaagaagt
    17341 gagcatccgg ggtacgcgtc tgcggcacgt ttgaacccca ggcgatgatg tagctggagt
    17401 tgtaccagtc agcagattcc ggtacgtcag tttgctcgcc ccaggtttgc ggagacgcag
    17461 gaggcaggtc gcagtaccag tcgtagaagc ttaagcaagt accgccaatc agcgagagat
    17521 agcgtgcacc cgatgcgtaa gaaaccatcg acattgccgg aattggcgag aaaccagcaa
    17581 cacggtccgg gccgtagttt ttgatggtgt aaacgttaga tgcggcgatc agttcgttca
    17641 cctcctgcca ggaagaacga acaaaaccac cgcgtccacg cgcctgctta aagcttttcg
    17701 ctttatcggc gtcttcaatg atagaagccc atgcctcaac cggatcgcta tgcagcgcct
    17761 tcgcttcacg ccacattttc atcaggcgtt tgcgcatcat cgggtatttc aggcggttgg
    17821 cactgtaaag ataccaggag tagctggcac cgcgcgggca gccgcgaggt tcatggtttg
    17881 gcagatccgg acgggtacgc ggatagtcag tctgctgggt ttcccaggtg accagaccgt
    17941 ttttgacgta gattttccag ctgcaggagc cggtgcagtt taccccgtgg gtagagcgga
    18001 cgattttgtc atgctgccaa cgctggcgat atccatcctc ccagtcacgg ttggtattga
    18061 gaagctggcc atgcccatcg gcaaaggttt cacccttctg tttgaagtag cgaaaccggt
    18121 ccaggaattt actcatcggt tttctcctgt gggagcctgt cggccctctg ataaatcgac
    18181 attgctaatg tttaagggca aggtaacgct ctgaaacagc gtgggaattg ataacgatca
    18241 agagtgatgg ggatgaaaaa taaagtaaat tcctaagata cccctttag
//
LOCUS       DAZGBC010000095        18283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGBC010000095 DAZGBC010000000
VERSION     DAZGBC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.095"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..1268
                     /gene="ybdN"
                     /locus_tag="ACHK1J_003157"
     CDS             48..1268
                     /gene="ybdN"
                     /locus_tag="ACHK1J_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7912392.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1241..1870
                     /gene="ybdM"
                     /locus_tag="ACHK1J_003158"
     CDS             1241..1870
                     /gene="ybdM"
                     /locus_tag="ACHK1J_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO7912393.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1871..3031)
                     /gene="ybdL"
                     /locus_tag="ACHK1J_003159"
     CDS             complement(1871..3031)
                     /gene="ybdL"
                     /locus_tag="ACHK1J_003159"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO7912394.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3140..4228
                     /gene="hcxA"
                     /locus_tag="ACHK1J_003160"
     CDS             3140..4228
                     /gene="hcxA"
                     /locus_tag="ACHK1J_003160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO7912395.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNNAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4238..4435)
                     /gene="ybdD"
                     /locus_tag="ACHK1J_003161"
     CDS             complement(4238..4435)
                     /gene="ybdD"
                     /locus_tag="ACHK1J_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7912396.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4617..6722)
                     /gene="cstA"
                     /locus_tag="ACHK1J_003162"
     CDS             complement(4617..6722)
                     /gene="cstA"
                     /locus_tag="ACHK1J_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO7912397.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKEMP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6903..7316)
                     /gene="entH"
                     /locus_tag="ACHK1J_003163"
     CDS             complement(6903..7316)
                     /gene="entH"
                     /locus_tag="ACHK1J_003163"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO7912398.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7319..8065)
                     /gene="entA"
                     /locus_tag="ACHK1J_003164"
     CDS             complement(7319..8065)
                     /gene="entA"
                     /locus_tag="ACHK1J_003164"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO7912399.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8065..8922)
                     /gene="entB"
                     /locus_tag="ACHK1J_003165"
     CDS             complement(8065..8922)
                     /gene="entB"
                     /locus_tag="ACHK1J_003165"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO7912400.1"
                     /translation="MAIPKLQAYALPEAHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8936..10546)
                     /gene="entE"
                     /locus_tag="ACHK1J_003166"
     CDS             complement(8936..10546)
                     /gene="entE"
                     /locus_tag="ACHK1J_003166"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO7912401.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAANNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFIAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10556..11731)
                     /gene="entC"
                     /locus_tag="ACHK1J_003167"
     CDS             complement(10556..11731)
                     /gene="entC"
                     /locus_tag="ACHK1J_003167"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO7912402.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQLLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11920..12876
                     /gene="fepB"
                     /locus_tag="ACHK1J_003168"
     CDS             11920..12876
                     /gene="fepB"
                     /locus_tag="ACHK1J_003168"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7912403.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGIHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12880..14130)
                     /gene="entS"
                     /locus_tag="ACHK1J_003169"
     CDS             complement(12880..14130)
                     /gene="entS"
                     /locus_tag="ACHK1J_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO7912404.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14241..15245
                     /gene="fepD"
                     /locus_tag="ACHK1J_003170"
     CDS             14241..15245
                     /gene="fepD"
                     /locus_tag="ACHK1J_003170"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO7912405.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15242..16234
                     /gene="fepG"
                     /locus_tag="ACHK1J_003171"
     CDS             15242..16234
                     /gene="fepG"
                     /locus_tag="ACHK1J_003171"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO7912406.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16231..17046
                     /gene="fepC"
                     /locus_tag="ACHK1J_003172"
     CDS             16231..17046
                     /gene="fepC"
                     /locus_tag="ACHK1J_003172"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7912407.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17043..18176)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK1J_003173"
     CDS             complement(17043..18176)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK1J_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO7912408.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4298 a         5184 c         4735 g         4066 t
ORIGIN      
        1 agtcttatct cactgatcac cctgtaacgt tcagagagcg tcttcagatg tctatttata
       61 aaattcctct tccgctcaat attctggaag ctgcacgaga gcgtattacc tggacactca
      121 acaccctacc ccgcgtttgt gtctcatttt ctggcggcaa agactctggt ttaatgctgc
      181 atctgacagc cgaacttgcc cgacaaatgg gcaaaaaaat ctgcgttttg tttatcgact
      241 gggaggcgca attctcttgc accattaact atgttcagtc cctgcgcgag ttgtacaccg
      301 atgtcatcga agagttttac tgggttgcgc tcccgcttac gacgcaaaat tccctttcac
      361 aataccaacc cgaatggcag tgctgggaac ctgatgtcga atgggtgcgt cagcccccgc
      421 aagatgcgat aaccgatcct gactttttct gcttttacca gccaggcatg accttcgaac
      481 aatttgtacg tgaatttgcc gaatggtttt cacaaaaacg tccggcggcg atgatgatcg
      541 gcatccgtgc ggatgagtcc tacaaccgtt ttgtcgccat cgccagttta aataaacaac
      601 gttttgccga cgataaaccc tggactacgg ccgcaccagg cggtcatagc tggtacattt
      661 accccattta cgactggaaa gtggctgata tctggacctg gtatgcaaat catcaaagtc
      721 tctgcaaccc actgtataac ctaatgtatc aggcaggcgt tcctctgcgt catatgcgaa
      781 tttgcgaacc ttttggcccg gagcaacgac aaggattatg gctctatcac gttatcgaac
      841 cggatcgctg ggctgctatg tgcgcacgag tcagcggggt aaaaagtggc ggcatttacg
      901 ccggacatga caatcatttc tatgggcatc ggaaaatcct caagccagaa catttagact
      961 ggcaagaata tgcattattg ctgctcaata gcatgccgga aaaaacagct gagcattacc
     1021 gcaataaaat cgccatttat ttgcactggt atcagaaaaa aggcatcgag gttccacaaa
     1081 cccagcaagg ggacattggc gcgaaagata tcccctcctg gcggcggata tgcaaagttt
     1141 tactcaataa cgattactgg tgtcgggcat tatcatttag tcctacgaaa gcgaagaact
     1201 atcagcgtta taacgaacgg ataaaaggaa aacgtcagga atgggggata ctatgcaaca
     1261 acgattaacg caggatttaa cgcaatttct cgccagcttg ccggaagatg atcgcatcaa
     1321 cgcgattaat gaaatccgca tggcgatcca tcaggttagc ccctttcgtg aagagccagt
     1381 ggattgcgtt ctgtgggtta aaaacagcca acttatgcct aatgattaca acccaaacaa
     1441 cgtggcacca cccgaaaaaa aactgctgaa aaaatccatt gaaatagatg gttttaccca
     1501 acccattgtg gtcacgcata cggataaaaa tgccctggaa atcgtggatg gttttcaccg
     1561 ccatgaaatt ggtaaaggct catcatcatt aaaattacgc ttaaaaggtt atttgccggt
     1621 gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg actattcgtc ataatcgcgc
     1681 tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc cgtgaactta gccagttggg
     1741 atgggacgat aataaaattg gcaaagagct gggaatggac agcgacgaag tgctgcgcct
     1801 gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt caatattccc gcgcctggac
     1861 ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc cagcaacgtc gattccttct
     1921 tggcaaaaca gagacgaatc agtttatgag ggaagggatc ggcgcaaaac accgatagcg
     1981 gaatcgccgc tacgccgtgc tcctgcgtca gccactggca aaactcaaca tcatccaggg
     2041 tagatacggc gctgtaatcc accagcaaaa agtatgtacc ttcacacggt aaaatctcca
     2101 gccggctttc atttaaagca ttcaccagaa tatcgcgctt ctggcgataa aagtccggta
     2161 acgcaagata atgctcaggt tctgcacgta gcatatcagc aagcgccagc tgtgccgggg
     2221 tattcaccga aaaggtcaga tactgatgta ccttgcgaat ttcggcactg atgggcgctg
     2281 gcgcaacaca ataacccact ttccagccgg tcatatgata ggtcttgcca aatgaagaaa
     2341 ccgccactgc ccgctcacgc agctgcggat gcgccagcac actggcatgg ccctgttgtg
     2401 aaaagttgat gtgctcgtag acttcatcgc taatgacaaa aatctcgtgc ccggcgatcg
     2461 cctgccacaa agcggcgaaa tcagcctgct gccagacagt tgcactggga ttatgcggag
     2521 tgttgaggat caccagtctg gtgcgctcgc ttaacaatgc ggcaaattcc tgccagtcaa
     2581 cgcgaaaatg cggtggttgc agtgccatac gcttcactat tcccccagaa agcgcgatgg
     2641 cgggggcgta actgtcatag ctgggatcaa aacaaatcac ttcatcgcca ttgcgcacca
     2701 gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc cgttacggtg atatcgctat
     2761 cggcatctgg ttgatagcca tacaaacgtt ccgttttctg agcaatcgcc tcgcgcaagg
     2821 cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg ggcaacgtgg tacgccagcc
     2881 gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc ttgcgacagg ttaatcgcct
     2941 ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt ggtgccaagt tgtggaagtt
     3001 tgctttgtgg aatcagaggg ttatttgtca tttttattgt acctgacgaa gttgcgtgtt
     3061 gaaggcacta taacacgatg ctagtatttg gcaatcaaga cgtttagatg tctaaataaa
     3121 acaataagga caacacaaca tgcctcacaa tcctatccgc gtggtcgtcg gcccggctaa
     3181 ctacttttca catccaggaa gtttcaatca cctgcacgat tttttcactg atgaacaact
     3241 ttctcgcgcg gtgtggatct acggcaaacg cgccattgct gcggcgcaaa ccaaacttcc
     3301 gccagcgttt ggacttccag gggcaaagca tattttgttt cgtggtcatt gcagcgaaag
     3361 cgatgtacag caactggcgg ctgagtccgg tgacgatcgc agcgtggtga ttggcgtcgg
     3421 cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc cgtctcggtc tgccatttgt
     3481 tgccgctccg acgatcgccg ccacctgcgc cgcctggaca ccgctctccg tctggtataa
     3541 caatgccgga caggcgctgc attatgagat tttcgacgac gccaatttta tggtgctggt
     3601 ggaaccagag attatcctca atgcgccgca acaatatctg ctggcgggga tcggtgacac
     3661 gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa ccagaaacgt tgccgctaac
     3721 cgtgcgactg gggatcaata atgcgcaggc cattcgcgac gtcttgttaa acagtagcga
     3781 acaggcgctg agcgatcagc aaaatcaaca gttaacgcaa tcattttgcg atgtggtgga
     3841 tgctattatt gctggtggtg ggatggtcgg tggtctgggc gatcgtttta cgcgtgtggc
     3901 ggcggctcat gccgtgcata acggtctgac cgtgctgccg caaaccgaga agtttcttca
     3961 cggcaccaaa gtcgcctacg gaattctggt gcaaagcgcc ttgctgggtc aggatgatgt
     4021 tctggcgcaa ttaactggag cgtatcagcg ttttcatctg ccgactacgc tggcggagct
     4081 ggaagtggat atcaataatc aggcggagat cgacaaagtg attgcccaca ccttgcgccc
     4141 ggtggagtcc attcattacc tgccagtcac gctgacacca gatacgttgc gtgcagcgtt
     4201 cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta gcagcagcgc gcgccgcctt
     4261 ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc ctcgtaggtc atcggcgttt
     4321 gatcgggatg gttaacccgc atatgttcga catagttgtc gtaatcaggc ataccaatca
     4381 tcagtttcgc cgcctgacct aaatattttc cggcttttgc cagtgaatca aacatcttgg
     4441 cctccgctaa ccatgttgct gaaagagtgc cggatgcggc gtgaacgcct tatccggcct
     4501 acaaatgcta tcaacgcgct cggtcagtcc cctcgcccca tcggggagag ggttagggtg
     4561 agggggaacc cacggcgcca tgccaacatt cgccaacatc ccccctcact ctgactttag
     4621 tgcgcgcctt ttgcctgcgc cacgatctcc tcgacatttt ccggcattgg ctcatacggc
     4681 atttctttcg ccgttggctt cggctctttc aatgccgcaa gtgccgtctt aatcgagaac
     4741 agtgccagaa ccaccacgac caccataaag aagatggtta acccggcatc cagacggttg
     4801 ttgaacacca gttgcgccag ctgtgactca gtatactgcg acggaatatt gccgctgtcg
     4861 atcattgcct ggaacttgtt agcaatggcc aggaagccga ctttcgcatc cgggctaaac
     4921 gctttctgcc agccagcggt cagggtacaa atcagcagcc aggccgttgg taccagcgcc
     4981 acccaggcgt aacgttgacg tttcatcttg aacagcacca cggcacagag catcagcgcc
     5041 atccctgcca gcatctggtt ggcaatacca aacagcggcc acagagtgtt aataccgccc
     5101 agcggatcga ccacgccctg atggaggaag tagccccacg ctagcacgca cagcgccgtt
     5161 gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc caggagacac cacgcccagc
     5221 agatcctgca acataaagcg cgcagcacgc gtacccgcat ctaccgccgt cagaataaac
     5281 aatgcttcaa acaaaatggc gaagtgatac cagaacgcca catccatcat gccgcccagc
     5341 gcgccgtgca gaatgtaggc catccccacc gccagcgtcg gcgcaccgcc cgcacgggaa
     5401 atgatcgact gttcacccac ttcgctggca atctggttta gcgtatccgg ggtaatcgca
     5461 aagccccagc tactcaccac ttgcgccgca gaagcgacca catccgccgt ccctgccgga
     5521 gccagcaccg ccatcgggct gttcatggcg aaatagacgc ccggatcgat gatacaggcg
     5581 gaaaccagtg ccataatcgc cacgaaggat tccatcaaca tcccgccgta gccgataaag
     5641 cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtac cggaagagat cagcgcatgg
     5701 aagccagaca ccgcaccaca ggcgatggtg ataaacagga acgggaacag gttaccggtc
     5761 cataccgggc cagtgccatc gacaaatttg gtcagcgcag gcatggtcag cgtcggacgc
     5821 ataatcaaaa tgcctaccgc cagaccgacg atagtcccga ttttcaggaa ggtagagagg
     5881 tagtcacgcg gggccagcag taaccacacc ggcagcaccg ccgccacaaa accgtagccc
     5941 accagcatcc aggtcagctg cacgccggta aagtcaaagt acggtgccca ggtcgggctt
     6001 tctgccaccc agccgccaga gataatggca aaaatcagga acaccaggcc aatcactgac
     6061 acttcgccaa tacgccccgg acgcagatag cgcaggtaga ttcccataaa aatcgccagc
     6121 ggaatggtga acgcaacggt atacgttccc cacgggctat gagtcagggc tttcaccacg
     6181 atcatcgcca gtactgcaag gataatgacc atgatcataa agcaggccac cagcgcgatc
     6241 accccggcgg ttggccccat ctcttctttg accagctcac ccagcgaacg accgtcacgg
     6301 cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac cggcgagaac caccccggcg
     6361 agtagccaga tcatccctgg caggtaaccc atttgcgccg ccagtaccgg cccaaccaac
     6421 ggacctgctc cggcaatggc cgcaaaatgg tgaccgaaca gcactttctt gtccgtcggc
     6481 acatagtcca gcccgtcgtt atggcgcacc gctggcgtca tacgcgtcgg gtcaaccgcc
     6541 agcacatttt tggcgatata caggccataa aaacggtaag cgatcagata gatacagacc
     6601 gacgccacca caatccacag cgcgttgatc tgttccccac gatttaaagc aatgtatccc
     6661 agagcaaatg ctcccattac agagagcact gtccagacga ggtatttccc tgatttgttc
     6721 atagttgtta tccgtgtgcg tgtccataga gatgttacat tttgtttcta taacatcttt
     6781 gtacgattta acaacacggc aaccatgtaa atcggagttg aaaccaatat ttaacttaac
     6841 aatgttaact cgatcactcc gttaaccgag ttttcctgca atctcattta attctgtcgg
     6901 gctcatccca aaactgccgt acccagccga caagtgcaac aacgccgccc ctgttcatcg
     6961 aaaacgacga tttcccagct ctgattttgc cgcccaagat gcagcggctg gcagacgccg
     7021 cgtaccttgc cttcagacac cgggcgatga tgggtggcgt taagttccgt gcccaccaca
     7081 cactgcccgt cacgggtcat cataaatccg gccatcgatc ccagcgtttc agccagcgca
     7141 gccgacgcgc cgccatgcag caggccaaac ggctgatgag tacgggtatc aaccggcatt
     7201 tcggcttcca gcacatcatc gcccagacgg gtatacacaa ttcccagatg cgccaccatt
     7261 gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat ggcgtttcca gatcatgctt
     7321 atgcccccag cgttgagccg ccatcgacca caatatcctg tagggtgata tggctggcga
     7381 ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg tggacgggcg attttcccca
     7441 gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat acgctgttct tcggcgtcat
     7501 cgctcaccca cagcgtgcgt tgcatatcgg tatcggtgga acccggcgaa accacattac
     7561 agcgcacgcc gctacccgcc agttccagcc cgacgctcaa cgccaggctt ttcagcgccg
     7621 ctttcgatgc gccataagca ctcataccaa tacgcggcgt gtgtgcggcg tcggacgcca
     7681 cagtgacaat cgccccgccc cgctgacggc gaaactggtt catggtttgc tggaacaggt
     7741 taaacgcacc gccgacgtta accgcaaaag tctgctgcca gtcctcctta ctgagctgat
     7801 cggtcgcgcc catgcgcaaa attcccgccg cattgaccag cacgtccagt cgctccgttt
     7861 cagctaacag tcgctgacat acctgcgcca cctgcgcagc atcggcaaca tccattactt
     7921 cggtcgcaaa gggatattgc tcctgagcga acgcttgatc aaaacctgta actttcgctc
     7981 ccgcttcaac aaacgccagc gccgtggcgt agccgatacc tttccccgcg ccggttaccc
     8041 agacattttt accgctgaaa tccattattt cacctcgcgg gagagtagct tccaccaggc
     8101 gtcgatggtc gggtttttcg ccaacatgac aaagtcgata tcgccatgca ctttgcgcca
     8161 gcgcgccgcc agcgccatca tgcgcaccga atccagaccg tagtcgatca ggttgtcgtc
     8221 atcgaacggt tcatcggact cgtccagcaa cggcaggatc acctcacgca gcgccgcttt
     8281 gctggcgggg ataggtgctg gcagtaattc ttcggtcatc accacccggc cagaacgtcc
     8341 ggccacatat ttcagcgaca tcaaatgctc gtcacggctg aaatcggcca gcgcatccgc
     8401 caccataaac ggtttaatat cgcgcataaa tgcgtcggtt gcggtggtca tacagccaat
     8461 gtgggcatat acccctgtaa taatcagctg gttacgtcca ctctctttga gcatttgctc
     8521 cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc agcaccgtgt cgtcggcatc
     8581 tggcgtcagg cgatccacca ccttttgctg ttccggcgag cgggtcaggc ccggccccca
     8641 catatcattc aacagcgccc gatcttcatc gctctgctct ttcggctggg cggtgtaata
     8701 aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca gcaatattcg cgatcacctg
     8761 ctccatcatc gggcagttct cgccccagaa gctgacaaaa tagtcctgca tatcatggat
     8821 taacaacgcg gcacgttgcg gttcaaaggc ccagtcgact ttattcttag ggatatcgtg
     8881 agcctccggc agtgcgtaag cctgtaattt tggaatagcc atcgtgttct ctccttcagg
     8941 ctgatgcgcg tgacgccagc cactgacgta attgtttttt atcgactttc ccgaccgccg
     9001 tcagcggaag tgaatccaca cactccacgc gatccggtaa tttaaattcg gcaataccct
     9061 gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg ctcttttacc accagataag
     9121 cacagctttt ttcgcccatc agctcatctt ccatactgac cagtgcggcg tagatcaccg
     9181 ccggatggcg cagcaacagg ttttcgatct cttcggcagc gatcttctcg ccgccacggt
     9241 tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc ctctggatca atagagatca
     9301 gatcgccgga acagtaaaaa ccgttggcat caaaggcgct ggcattgtgc tgcgggcttt
     9361 tgtaatagcc acggaaggta tacggcccgc gcgtcatcag gcgtccgact tccccttgcg
     9421 gcagtggatt tccttcggca tcggcaaccc atacttcgtc atccggacac attgggtaac
     9481 cctgggtatg gataattttc tccgcgctat catcaagacg ggtatagttc accagccctt
     9541 ccgccatacc aaacacctgc tgcaactggc aaccaatctc agcgggaata cgcgccgcaa
     9601 gcgtggcaga aagacgtgcg ccaccgacct gtaacagttt cagcgaggca agctgcgccc
     9661 ggctttcgcc ttcggtcagc gcctgcaacc acaggctgac tgccggcggc accagcgcgg
     9721 taacgttaat ctgatgtttt tcaatcaatg ggaagcaaag cgtggcgctg ggatcggccg
     9781 ccagaacaac cgttcctccg gcaagaaaga cgcccagcga tcctggcgaa ctcatggcgt
     9841 agttatgagc cgccgggatc gcgcacaggt agcgcgtctg ttgtgtgaac tgacaaatct
     9901 cgacgctacg acgcacgctg tagtagtagt cgttatgagt gcgcgggatc agtttcggtg
     9961 tgccggtggt gccgccggaa agctggaaat aggccacttc atcagcaggt gacggcgtgg
    10021 cagtaaaatc ctcagccgga tggttaatcg catcctgcaa gttatgctca ccgctgtcgt
    10081 tgtgcagttg caccacgcga atggaggagt gctctgcaat aaatgtattg aggaaatcat
    10141 ccccgctaaa tagcgcatgt tggcgatcgg caatcagtaa cgccggttca atctggctgg
    10201 cataggcgtt cagttcacta cgctgatggc tgaacagcgc cagcaccggc gcaacgccca
    10261 gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac gttacccagt tgtaccagcg
    10321 cggtttcacc gggtttaatg ccctgacggc gcaaactaca ggcgagatta ttcgccgcct
    10381 gattcagctc ccgataactc aactgccgct cgccgtcgat aaccgcgatg ctgtcactcg
    10441 cagcgtggcg agtcagaatg tcagtcagtg gcaaatcctg ccagtagcct ttttcccgat
    10501 agcgacgggc aaactcttcc ggccagcggg taaatggaat gctcatcatc gctccttaat
    10561 gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc tgtttcgcgc cactcaccca
    10621 acggtgacga cgcaggcaca atccccgctc cggcaaacag acgcacctga ttttcccgca
    10681 gcttcgcgca gcggatggtc accacccatt cgccgttacc ttcgctgtca caccaaccca
    10741 caatgccgcc aaacagttcg cggtcgaacg gctccagttc agcaataacc tgggtcgcgg
    10801 cctgatgtgg gaaaccgctc agcgcggggg tcggatgcag cagacaagcc agagtcagtg
    10861 cgttttcttg cgaattcgct ttaccttcaa agggagttgc gagatgccac agcgtcggcg
    10921 tggtaatcaa ttgtggagag gagggaacgt gtaactcact actgcgttcg cgcagtacct
    10981 ctttcatcgc ctgagtcacc agttcatgtt catggcgatc tttttctgac gccagcagac
    11041 gattacccgc ttcgcgatcg agcacttcat ccggctgacg acgcgcggaa ccggctaacg
    11101 gaatggagct aaaacgctcg ccgtctttac gtagcagcag ttccgggctg gcccccagca
    11161 gaacgccacc atctgccaac ggcacatgga agttgtaact aaccgggttt tgcgcaatca
    11221 accgttccag caatacgcca ctatcaatgg cggcgtcagt ggtgatatca atcaaccgtg
    11281 acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc ggcggcgcgg gcaaccatct
    11341 gttcaaacgt ggtttgttcc ggaattgcct ggcgttccac cacattcagc aactggctgc
    11401 gggtgaaacg gcgtgcggaa gcttgttttt cctgacggga gaacgactgc caggattcgg
    11461 gaatatacag cgacgaaggc tgacgtggat cgaagggaat cgccccgacc atcaccggat
    11521 ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc gagtttttgc tggaagggac
    11581 tgtcaggcga atccccgttc acagccggtt catcgaagcg ggcgaaacat cctgacgtcg
    11641 taaaactgcg gtacggcgac ataaagaaaa agcgattggg cgcaagtgtt gccatggtct
    11701 gctgtacttc ctcagccagt gacgtatcca tatcatcctc cacaaaatga taaaggcttt
    11761 aataatgatt atcatttata ttttcggtcg ctaacctaaa agcaaacgcg cactatgtca
    11821 actcttgagg taacgcaatc tgcaactcgg ggttgcatct ggttgtggtt acaatgaaaa
    11881 tgagaagcat taacttcatt aataacagga agctgatttg tgagactcgc cccgctctac
    11941 cgcaatgccc ttctattaac aggacttttg ctttcaggaa tagccgcagt tcaggccgcc
    12001 gactggccgc gtcagattac tgacagccgt ggcattcata ccctggaaag ccagccgcag
    12061 cgtattgttt ccaccagcgt caccctgacc ggctcactgc tggcgattga tgctccggtg
    12121 atcgccagcg gcgcgaccac gccaaataac cgcgtcgcgg atgaccaggg ctttttacgc
    12181 cagtggagca aggtggcgaa agaacgcaaa ctgcaacggc tctatatcgg cgaaccgagt
    12241 gcagaagccg ttgccgcgca aatgccggat ctgattttaa ttagcgcaac cggcggtgat
    12301 tcggcgctgg cgctgtacga tcagctttcc accatcgcgc cgacattaat catcaattac
    12361 gacgacaaaa gctggcagtc gctgttaacg cagcttggcg aaattaccgg gcatgagaaa
    12421 caagcggcag agcggattgc acagtttgat aagcaactgg cggcggcgaa agagcaaatc
    12481 aaattaccgc cgcagccggt cactgccatt gtctataccg ccgctgcaca cagtgccaat
    12541 ctctggacgc cggaatcagc acaagggcag atgctggaac aactcggctt tacgctggcg
    12601 aagttgcccg caggcttaaa cgccagccaa agccagggca aacgccatga catcattcag
    12661 cttggtgggg aaaatctggc tgcagggtta aatggcgagt cactattcct gttcgccggt
    12721 gatcagaaag atgccgatgc tatttatgct aatccgctgc tcgcgcacct gcctgcagta
    12781 caaaacaagc aggtttatgc gctgggaacc gagacgttcc gtctggatta ctacagcgcc
    12841 atgcaagtgc tggaacgact taaggcgctg ttttaagcat taaccgtcag acgctgtcac
    12901 ctgcggcggc gtctggcgaa aacgtcgcaa ctccaccagc accagcaata acaacacgcc
    12961 gataatcaac aaaccaaaac cgcttgcgct tgcggaagca accggtgtca tcatcgctcc
    13021 caaaccgccc agcagcgccg cgcctatggc atcgcccgtc acgttttgtg ccgtccacaa
    13081 accgttaatc cgccctaaca tcgcttccgg ggtttgcgtt tgcagcattg tgtattgcag
    13141 caacgagctg accgcactca accagccgaa cagcgccaga caaaccacgc ctaaaatcca
    13201 catcggcatc aggccaaaca gaccaatggc gaggaacgat cccagcgtgg agagcagcat
    13261 caataaccct ggtcgcgcgc tatgcgccag cttcccgctg gttaacgcgc caatagccgc
    13321 gccgagcggg atcgccgcat agagaaaacc aatctgtgcc gccgacatct gccagttgtc
    13381 agccagcgcc ggatacagta cccgcaccgc actcgccatc gtcaataaac cacccagcag
    13441 cgcaatccct cctaccagcg ggctggcgag cagaaaacga aatcctgcca gtaatgattt
    13501 caacggatgc tcacgcggct gcggtggcgg tggcaacgcc ggaaggctta acaacggcag
    13561 caaggtaata aacgtgcccg ccgccgccag tccgtagttc caggccacac cgccagtcgc
    13621 cagcagtaaa ccgccaatca tgggcgaaat caccgacccc agacgcacgg tcaacatggt
    13681 gatcgccccg gcctgcatta agttttcacg ccctaccagt gcaggtgtcg ccgccagtag
    13741 cgccgtaacg ccaagtgatg cgaaaaaacc atcccataaa ccaagtaaat agattgccag
    13801 caatgacggt tcaggcagca gtgcattaag gcacagtcca atgaagccaa tgccacaggt
    13861 gccgcgcgcc agcaaaatca cttttttgcg ttcatagcga tccgccagca caccgccgac
    13921 catcaggcca acaaacatcg cgccgccggt cagcgtcacc gaaagcccca cctgccaggt
    13981 ggaatgtgtc atcatctgga tctgcaccgg caccgcgacg ccgagcaaac ccagagaaac
    14041 aatagagatg aaacgagcga ggaatactgc gcgaaacgcc gggtgcgttt tcaacaggct
    14101 gaggttaagc agccaggatt gtttattcat tacaatgcct tgccatcaaa tattttaaat
    14161 acctattctt aggctgcaca tgctaacata tccaaataag atcgataacg ataattaatt
    14221 tcattatcat ggaagttcgt atgtctggtt ctgttgccgt gacacgcgcc attgccgtgc
    14281 ccggattgct gttattactg attatcgcga cggcattaag tctgctcatt ggggcaaaat
    14341 cactccccgc ttccgtagtg ctggaggcct tctccggcac ctgccagagc gccgactgca
    14401 ccatcgtgct cgacgcgcgg ctgccgcgta cccttgccgg tttactggca ggcggcgcgc
    14461 ttggccttgc cggggcgtta atgcaaaccc tcacccgaaa cccacttgcc gaccccggct
    14521 tgcttggcgt gaacgccgga gccagctttg ccattgtgct gggtgcggcg ctgtttggtt
    14581 actcttccgc acaggaacaa ctggcgatgg ccttcgccgg ggcgctggtg gcctcgttga
    14641 ttgttgcctt taccggcagc cagggcggcg ggcagttaag tccggtgcgt ttaaccctgg
    14701 cgggcgtggc gctggcggca gtgctggaag ggctgaccag cggcatcgcc ctgcttaatc
    14761 ctgacgtcta cgatcagttg cgtttctggc aagccggttc gctggatatt cgcaatctac
    14821 ataccttaaa agtggtgctg atcccggtgc tgatcgccgg agcaaccgcg ctattactga
    14881 gccgcgcgct gaacagtttg agcctcggca gcgacaccgc gacggcgctg ggcagtcgcg
    14941 tggcgcgcac acagttgatt ggtctgctgg cgattaccgt gctttgtggt agtgcgacgg
    15001 cggtagttgg cccgattgcc tttatcggcc tgatgatgcc gcacatggcg cgctggctgg
    15061 taggtgccga tcatcgctgg tcgctgcccg tcacgctact tgctacccct gccctgctgc
    15121 tgtttgccga tatcatcggg cgggtgattg tgcccggcga actgcgcgtt tctgtggtca
    15181 gtgcgtttat tggtgcaccg gtgctgatct tcctcgtgcg acgtaaaacg cgaggtggtg
    15241 catgatttac gtctctcgcc gattactcat cacctgtttg ctgctggttt ccgcctgtgt
    15301 ggttgcaggt atctggggat tacgcagcgg tgccgtcacg ctggaaacct cgcaggtatt
    15361 cgccgcgctg atgggcgatg cgccgcgcag tatgacgatg gtggtcaccg aatggcgttt
    15421 accacgcgtg ctgatggcgc tgttgattgg cgcagcactg ggcgtcagtg gcgcgatttt
    15481 tcagtcgctg atgcgtaacc cgctcggcag ccctgacgta atgggtttta acaccggggc
    15541 gtggagcggc gtgctggtgg cgatggtgct gtttggtcag gacctgacgg ctatcgcgct
    15601 ggcagcaatg gtgggcggca ttatcacttc gctgttggtc tggctgctcg cctggcgtaa
    15661 cggtatcgac acctttcggt tgattattat cggtatcggc gttcgcgcca tgctggtggc
    15721 ctttaatacc tggctgttgc tgaaagcgtc tttagaaacg gcgctaacag caggtttgtg
    15781 gaatgccgga tcgctcaacg gcctgacgtg ggcaaaaacc tcgccttccg cacccatcat
    15841 tatattgatg ctaattgccg ccgccttact ggtacgccgg atgcgcttgc tggaaatggg
    15901 cgatgatacc gcgtgtgcgc tgggcgtcag cgtcgaacgt tcgcgtctgt taatgatgct
    15961 ggttgcagtg gtgcttaccg ctgcggcaac agcgcttgcc gggccgattt cctttattgc
    16021 tttagtcgcg ccgcacattg cccgacgcat tagcggcacc gctcgctggg ggctaaccca
    16081 ggcggcgctg tgcggggcgt tgttactgct ggcggccgat ctctgcgccc aacaactgtt
    16141 tatgccgtat caacttccgg ttggcgtcgt taccgtcagc ctcggcggta tttaccttat
    16201 cgtcttgtta attcaggagt ctcgcaaaaa atgaccgaat cagtagcccg tttgcgcggc
    16261 gaacagttaa ccctgggata tggcaaatat accgttgcgg aaaatctgac tgtagaaata
    16321 cctgatggtc acttcacggc aattatcggg ccaaatggct gcggtaaatc cacgttgctg
    16381 cgtaccttaa gccgcctgat gacgcctgct cacgggcatg tctggctgga tggcgagcac
    16441 attcaacatt acgccagtaa agaagttgca cgccggattg gtctgttggc gcaaaatgct
    16501 accacgccgg gcgacatcac cgtgcaggag ctggtggcgc gtgggcgtta tccgcatcaa
    16561 ccgctgttta cccgttggcg caaagaagat gaagaggcgg taacgaaagc gatgcaggcc
    16621 acgggaataa ctcatcttgc agatcaaagc gtggataccc tttccggtgg gcaacgccag
    16681 cgagcgtgga tcgcgatggt gctggcccag gaaacggcga ttatgctgct cgacgaaccc
    16741 acgacctggc tggatatcag tcatcagatt gatctactgg aactgttaag cgaactgaac
    16801 cgcgagaaag gctataccct ggcggcggtg ctgcacgatc ttaatcaggc ctgtcgttac
    16861 gccagccatt tgattgcatt gcgggaaggg aaaattgttg ctcagggagc gccgaaggag
    16921 attgtcactg ctgaattgat cgagcgcatt tatggtctcc gctgcatgat tattgacgat
    16981 ccggtagccg gaacgccgct tgtggtgccg ctcggacgaa cggcaccttc aaccgcaaag
    17041 atttaaacta agtggtctgc catcatggca tcctgctttc tggatgccat cgcatagcgc
    17101 aataacacgc taccacaagc caccatcccg ccgatcagcg cggaaaggat cacaataatc
    17161 gccttacccg gaccgtcttt tttcactggc aatgacgggc ttaactgata tttaaacggc
    17221 gtaaaattca catcgttgat atttgctttt gttaactgct caacaagata ctgacgatta
    17281 cgtaattcac cgttcagttc cgcaacgtca gtgaccgctt tttcaatttc cagtttgcgt
    17341 tcaataccgt ctgcgccgag agaaatagag aaatcggggt catctttaac ggcctgacca
    17401 ttactgtaaa cgggcttttt aattcctgcc gcgttggcaa tgtcgagtga ataattgagg
    17461 cgctgaatgt ttgcatcaag ttgatttttc attttaatgc ggtcctgagc cagtttttct
    17521 ttttcaaact gggttttgat ctccagttta ttacggacgt tttctatcga ctctttcacc
    17581 accaacgcag agatataatc gatatacccg ctcaaaacgg tctgcgcctc ttcactggtt
    17641 ggcgcggtaa aacttagcgt ccaggaggta tacagtgacg gttcatcttt tttcttactg
    17701 gcattgtcat caaccgcttt cattttttcg ctcaatgcga caattgcgcg atgcaaatcc
    17761 agttcgtcga ttttcgcctc ttttaattgg tccatcacat aaggtgatga acgcaggtac
    17821 tcttccagca agctaaccga ctgaaacttc ttgataaaca ggttaaatgc ttctgtacga
    17881 tcaattttga tatccagatc cagcacacga agcttagtga atgttttctc caactcttgc
    17941 cactgaacag gttctggagg cgtgacaacc gccgcgctgg tccatttttg cggcaggatg
    18001 aaagagatca gcaagcctgc gcaggcaaac gcaaaaacga ccgccatgac cgtttttttg
    18061 gcccgccata aaactgagat tagattaagt aaatcaattt cattattact gggcgacgcc
    18121 agaggataat cgggaaaatg agcgtcactt ccctgtttaa tattcagtga tgacatgggg
    18181 tataacctga aaatgagtcc aataggggga aatctccacg gcgctcactt taccagttgg
    18241 tgagaattat ccgaagctga agtttgtaaa ttcattccac tga
//
LOCUS       DAZGBC010000096        18145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGBC010000096 DAZGBC010000000
VERSION     DAZGBC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.096"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1945
                     /locus_tag="ACHK1J_003174"
     CDS             <1..1945
                     /locus_tag="ACHK1J_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO7912409.1"
                     /translation="NVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARG
                     IIVSDKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISN
                     AYPDYDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFL
                     THTESLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHLLTDELIKDEAVARGLVKNSNI
                     SADVNKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLNVRKTLLSHYDYAWLVPT
                     AVADELINSEDPELRERITGADLTAQQAVLLSKDKSLKVREALARTLTELKITQLSAT
                     LRTEDIERIAEQMYLDNKENKNIVKALLIALPEMRQLSLAKEDVHNLREGTRYLTSKE
                     VISYLLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLAL
                     IDNGVVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWA
                     LAVVSMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQT
                     ALRNAHTPASLLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSLLR
                     DLVKTGATRKIRSEARHRLEEKQ"
     gene            1942..2397
                     /locus_tag="ACHK1J_003175"
     CDS             1942..2397
                     /locus_tag="ACHK1J_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912410.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(2475..3386)
                     /gene="rdgC"
                     /locus_tag="ACHK1J_003176"
     CDS             complement(2475..3386)
                     /gene="rdgC"
                     /locus_tag="ACHK1J_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO7912411.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3511..4419
                     /gene="mak"
                     /locus_tag="ACHK1J_003177"
     CDS             3511..4419
                     /gene="mak"
                     /locus_tag="ACHK1J_003177"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO7912412.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4553..5737)
                     /gene="araJ"
                     /locus_tag="ACHK1J_003178"
     CDS             complement(4553..5737)
                     /gene="araJ"
                     /locus_tag="ACHK1J_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO7912413.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(5863..9006)
                     /gene="sbcC"
                     /locus_tag="ACHK1J_003179"
     CDS             complement(5863..9006)
                     /gene="sbcC"
                     /locus_tag="ACHK1J_003179"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO7912414.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTVPQEDEEESWLATRQQEAQSWQQHQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALVDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPGGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(9003..10205)
                     /gene="sbcD"
                     /locus_tag="ACHK1J_003180"
     CDS             complement(9003..10205)
                     /gene="sbcD"
                     /locus_tag="ACHK1J_003180"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO7912415.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            10395..11084
                     /gene="phoB"
                     /locus_tag="ACHK1J_003181"
     CDS             10395..11084
                     /gene="phoB"
                     /locus_tag="ACHK1J_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO7912416.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11142..12437
                     /gene="phoR"
                     /locus_tag="ACHK1J_003182"
     CDS             11142..12437
                     /gene="phoR"
                     /locus_tag="ACHK1J_003182"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO7912417.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            12844..14163
                     /gene="brnQ"
                     /locus_tag="ACHK1J_003183"
     CDS             12844..14163
                     /gene="brnQ"
                     /locus_tag="ACHK1J_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO7912418.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14239..15612
                     /gene="proY"
                     /locus_tag="ACHK1J_003184"
     CDS             14239..15612
                     /gene="proY"
                     /locus_tag="ACHK1J_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO7912419.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            15768..17585
                     /gene="malZ"
                     /locus_tag="ACHK1J_003185"
     CDS             15768..17585
                     /gene="malZ"
                     /locus_tag="ACHK1J_003185"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO7912420.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGSQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            17773..>18145
                     /locus_tag="ACHK1J_003186"
     CDS             17773..>18145
                     /locus_tag="ACHK1J_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912421.1"
                     /translation="MTISNSNLLKPFITGAELPPFKEDVFSKTEEQFKKSTHGKSLFI
                     CGNENLVNISAEKIDEIKNSKNFFSNVVNIIILLIKYPDKILPILHGCPAESIELIKN
                     AKRTGQWSYEMTDKNGQNIEVS"
BASE COUNT         4234 a         4463 c         4814 g         4634 t
ORIGIN      
        1 taatgttatt gtcgataact ttattaatca tgatttcgcg tatgtcaaag gacagatgac
       61 agcgaaaggt ttagtttatg ctgattacaa tgatcataac tttgaagtga tgaagggaat
      121 tagtgcgcga ggcattattg tttccgataa agcaacgcaa tttgaagtca ttaaagctga
      181 attttatatc aatgaagatg gaagtggtga aggatataac tgggatgaga atattcaaaa
      241 agcctattca ctggtgactg ccgatcttta tgatcacact gaaatcgaaa ccgataacat
      301 ttccaatgcg tatcctgact atgatagcgt cgcagacaac atcgttcagg gtttaccgct
      361 ttttcgagat aaagccgctc ctgaaattaa tgagaaatta aaatggattg aaacggggaa
      421 actcgataat ttcccagcga ataaaataaa gcatcaagat ccactggtgg cacgttttct
      481 gactcataca gagagtttat cacccgccgt tatgctgcaa ctgttacagc accccgatga
      541 ccagacgcgt gaatcgatgg cgcagtcctg gcccgctcaa cagatgcatt tgcttaccga
      601 tgaattaatt aaagatgagg ctgttgccag agggctggtt aaaaacagta atatttccgc
      661 cgatgtgaat aaaaaattaa tgtctgtacc cgtcgagagc gtacagctgg agcaggccag
      721 acaggataat ctttctccag atattgttgc gtcgttaagt catagcccct ttctcaatgt
      781 acgcaagaca ttactcagcc attacgatta cgcctggctg gttccgacag cagtggccga
      841 tgagttgata aatagcgaag atccggagtt acgcgaaaga attaccggag cagatttaac
      901 cgcacaacaa gccgtgctgt tgagtaaaga taaatcactg aaagttcgtg aagcattagc
      961 ccggacgctt actgaattaa aaattaccca actgtcagca accctgcgta cagaggatat
     1021 tgaacgtatc gcagaacaaa tgtatctgga taataaagaa aataaaaata tcgtaaaagc
     1081 gttattgata gcgttgccgg agatgcgtca actgtctctg gcaaaagaag atgtgcacaa
     1141 tttacgcgaa ggtacgcgtt atctgacatc gaaggaagtg attagttatc tactgactca
     1201 gcatgatgtt cccaccgtct gggatgagct ggcacgcgat aaattattgc cgctggaata
     1261 taagaaacag ttatggcaac gcacattaaa tttaatgatg agcaaacggc aagaagatca
     1321 agagcaagcc tacgaagtac agctggcgct gattgataat ggtgttgttg acgaagagat
     1381 gttaaataat gccattgatt tactggttga tttacccgct gaataccgct atcgaatgcg
     1441 taaccaatta tttgataata aagaattacc ttccggaatt atcaacaaac tcgatcaaca
     1501 gtatcgcttc aactctgact gggcgctggc tgttgtcagt atgaaaaata gcacccgtag
     1561 acaaagtgaa agaggattac accgctggaa ccatgaggat agcgatattt ttgcggaact
     1621 ggcgacgata aaagataaat cagatgatga atggtggcgt gcattgctgc aaagtcgcaa
     1681 tgatcatttg cgccaaacgg cattacgcaa cgcacatacc ccggcgtcgt tgttaacgac
     1741 attgactgag cctcaggatc ggtcgctggc tattaataat ccacagctgg ctgccgatgt
     1801 gaaaacggcg tggttaaaag aggatccatc attactctta tttgtcgaac aacccgatct
     1861 ttcgctgtta cgtgatttag tgaaaaccgg ggcaacgcgg aaaattcgca gtgaagcgcg
     1921 tcaccggctt gaggaaaaac aatgacacag cgcccatgga gcaagttaca gcgggagatc
     1981 tatgacttac tgacgccaac gatcaacctg caaattcact gtacccgtta tcccatgcgc
     2041 agccaaaatg gtggaagcac cgatttaccg cgttattgga tcacgttgga taaaaacgtg
     2101 atctgggatt acccgaaaga ctttattgca ggtaacggtg gagtgcgtaa ttttcacggt
     2161 gaaacttgct ggtatcctta cctgacggat atttgttcaa tttctgattt attgcgtgaa
     2221 tatattgaca ccccaaaagc ggagctgtta acaaaacaat ttaccagcga taaatggggg
     2281 ttagtgaata ttctgcgtgc tgctgacagg cgaataggga tgaggcgatt agatcagtta
     2341 cggcgtaaaa cgcataatat tgctgcgcta aaaattattg cccgccgtag cgaataatta
     2401 tatgcctggt gtggcttcgt acgccggata agacgcggca ggcgtcgcat ccggcattaa
     2461 aggtaaatca gcaattaacg ttgtgcttcg ccacctaatc cttcaatcag gttttgaatt
     2521 aacgctgcca gttcaccagt catcaggatg aaatcggcat caaaacgctg ggcgaaatct
     2581 tcacggtcga tatcttcgtt ttgatcgcgc agctcgtcgc agaacttcag acgcttgagc
     2641 gaaccatcgt cgcacatcac aaactgaatg cgctgctgcc agtcgagcgc cagtttagtc
     2701 accacttttc cggcttcaat gtgattggtg atctcttcgc tggtcagatc ttgtttcttc
     2761 gcgcggatca cgccgccatc ttccagcaac gatttcagct cggcttcatc aagcagctgg
     2821 aagccctgtg ccgcactacc ggaacgtacc cattcggtca gcgtcagttc aatcgggttt
     2881 tccatgctca atggtacaac cggtaacgac cccaggcttt tacgcagcag tgccagcgta
     2941 tcttccgctt ttttggcact ggcgcagtcc accataatca aaccgttaac cgtgtcgatc
     3001 cacatcattg tctggctaaa acggctgaaa gcacgcggca acagagagtg cagcacttcg
     3061 tctttcagcg aatctttttc ggttttcttc agcttacgcg cctgttccgc ttccagtttg
     3121 gcgattttcg cttccagcgc ctgtttaatc accggagacg ggaggatttt ttcttctttg
     3181 cgtgcgcaga taacaatttg accattggca acgtgcgtta acgcatcgct gtgcgatccc
     3241 atcggaggaa cccagcccat cttcgccatg tcctggctgc cgcatggggt aaatgccatc
     3301 gaggctagct gtttttccat ctcttctgca cgcagcgaaa tctcgcggct aagacggtaa
     3361 accattaaat tcttgaacca cagcatgata atttccacgg ccttgtcgtt aaatttagcg
     3421 ggcatgataa cgaattgtcg gcggccttgc attgccaatc cggttgtccg tctctacgct
     3481 attgatattg aaaaaaataa ggagagtacc gtgcgtatag gtatcgattt aggcggcacc
     3541 aaaactgaag tgattgcact gggcgatgca ggggagcagt tgtaccgcca tcgtctgccc
     3601 acgccgcgtg atgattaccg gcagactatt gaaacgatcg ccacgttggt tgatatggcg
     3661 gagcaggcga cggggcagcg cggaacggta ggtatgggca ttcctggctc aatttcgcct
     3721 tacaccggtg tggtgaagaa tgccaattca acctggctca acggtcagcc attcgataaa
     3781 gacttaagcg cgaggttgca gcgggaagtg cggctggcaa atgacgccaa ctgtctggcg
     3841 gtttcagaag cagtagatgg cgcggcagcg ggagcgcaga cggtatttgc cgtgattatc
     3901 ggcacgggat gcggcgcggg cgtggcattc aatgggcggg cgcatatcgg cggcaatggc
     3961 acggcaggtg agtggggaca caatccgcta ccgtggatgg acgaagacga actgcgttat
     4021 cgcgaggaag tcccttgtta ttgcggtaaa caaggttgta ttgaaacctt tatttcgggc
     4081 acgggattcg cgacggatta tcgtcgtttg agcggacatg cgctgaaagg cagtgaaatt
     4141 atccgcctgg ttgaagaaag cgatccggta gcggaactgg cattgcgtcg ctacgagctg
     4201 cggctggcaa aatcgctggc acatgtcgtg aatattctcg atccggatgt gattgtcctg
     4261 gggggcggga tgagcaatgt agaccgttta tatcaaacgg ttccgcagtt gattaaacaa
     4321 tttgtcttcg gcggcgaatg tgaaacgccg gtgcgtaagg cgaagcatgg tgattccagc
     4381 ggcgtgcgcg gcgctgcgtg gttatggcca caagagtaaa aaacccaggc aattggcgca
     4441 tcatgcctga tgcgacgctt gccgcgtctt atcaggccta caaaatgtgc cagaaccgcg
     4501 taaggcgttc acgccgcatc cggcaataag caacgtgctc cgtgactgca atctacccca
     4561 gaggtttcgc cagcaccgga gtatccgccg cttgctggcg cttatagcga ccatacagca
     4621 gcaatgacga catcgcagca aacgaaagca gggcggcagg cagcgccacg taattatatg
     4681 ccagccccag cgtcagcatc ataccaccgc aatatgcgcc gacggcgcta ccgaggttaa
     4741 aggctatttg cccacctgcg gcacctaata actctccgcc tttggcgttt tgcagtaaca
     4801 atatttgcag aggtgctgaa agggcaaata atcccgcgca acaaataaaa gcaaaaataa
     4861 gcgacgttgt tttcataccg ccgcagaaaa agagcatcag cagtgccagt acaattataa
     4921 agtcagtcac tgctgcaatg cgcagtggtg aataacgtcc tgaaatcctg ccacttagca
     4981 tatttcccag caccatccct agcccaacta acatcataat aaaggtcatc gccgtttccg
     5041 aaaaaccgga aataaacatc atgtatggct ttacgtagct gaaccaggca aacacacctg
     5101 cgttgccaaa catggtggcg gcgaaaatta accacggggc cgggctgcgc aaaaagtgaa
     5161 attgttcgcg cagctttcct ttcgcctcgt cgcgaatatc cggcacccaa aaatagaccg
     5221 atgccatcac cgcaatatta aaaacagcga tcaataaaaa ggtgtaacgc cagctaaatt
     5281 cctgacttaa atacgttccc agcggaatgc ccagcaaatt ggcgactgtc atcccggaaa
     5341 ccatccccgc cacggcggcg gtgacttttc cgggtttgat aatttttgat aacacgatcg
     5401 ctccgacgcc aaaaaatgcg ccatgcggaa agccggaaac caggcgacca atggcaagca
     5461 tcaggtaaga cgaagagagc gtgaacatgg cgttgccaat gacgcacaac gccaccagaa
     5521 acaacaagat atgtttgagt gagtagcggc tggaaaagag tgcgattatt ggcgcaccga
     5581 ccaccacccc cagcgcataa tacgagatca tatgcccggc agcaggaatc gaaattccta
     5641 cgttatgcgc cagctccgtg agcacgccca taatgccaaa ttcggccatc cccaaaccaa
     5701 acgtgcccag agccaaagat aaaatgactt ttttcatacc accatcacaa ccaccagaac
     5761 ggctaccaat caccgtagcc aataaagtga tagcttgcag gataacgacc agcaaggcca
     5821 agagataatt cgcccctctg tattcattat cctgctgaat agttatttca ctgcaaacgt
     5881 actttccagt ttgctgtagc ccagtccatt gatctttttc actttgatct gcaccggaat
     5941 acgctctttc atcgcttcta cgtggctaat cacaccgatg gttttgccac tggcgttcag
     6001 ggcatccagc gcatcaaggg cggtatccag cgtttcgcta tccagcgtgc caaaaccttc
     6061 atcaaggaac agcgagtcaa tacgtgtttt atggctgacc agatctgaaa gcgccagtgc
     6121 cagcgccaga ctgacgagga aactttcgcc gccggaaagg gtacgggtat cgcgtaccgc
     6181 atctgcctgc caggtatcaa caacctcgac ttccagcgcc tcgctggctt tgcgctgtaa
     6241 cagatagcgc ccgtgcagcc gggtaagttg ctgattagcg agatggacta aattatccag
     6301 cgtcagcccc tgggcaaatt tgcggaattt atcgccctct ttggaaccta ttagcgaatt
     6361 cagatatccc cagtcctcaa cctgctgcgt catttgagca atttgctgca ttaaggtttg
     6421 ttgttgctga cggttatctg catcctgctt cagctgctgg cgaatctcgc cttgactcgt
     6481 ggtgttctca cgcaattttt ggtgagtttg cgctaactct tgctgaatct gctccaccgt
     6541 cacagtgaga gccaacccgc caggtcggtg ttgctgatgc tgtgccagcg tttctgctgt
     6601 ctgagtgacc agcgtttgcg cctgacggcg ctggttttcc agattctgct tgagctgttc
     6661 cagctgcgtt agtgtttgtt catccactag cgccgcaagg aacgcctgct gatcgtcaaa
     6721 gacgctggcc tgtagcgcgg tgtcaaactg cgcctgggct ttttgcagac tttgcgccgc
     6781 cagaacatcc tgttgctgta acgtctgctg ctggctgtgt aatgcgagac attgttcatg
     6841 tacctgccgc cagttttcca ataccacagt ttcttcgcag tgcgggagtt catcactttg
     6901 cggcaacgtt tccagaatcg gcgtcagctg ctgaatacgg ttttgcagcg cggttaattc
     6961 gttctggtgt tgctgccagc tctgcgcttc ttgctgacgt gtcgccaacc agctctcttc
     7021 ttcatcttcc tgtggcactg tcagtgcata acccgccaat gccgttaaga gctgctgctg
     7081 gcgttgttca atttgctgtt gatactggat aatttgctga ttatgcgcgg caatctgccc
     7141 ttgtaattca tgtcgttggc tgagtaaccg cagctggcgt tcgtgctcat cttgtgcatc
     7201 cagccacggt tgaatatcgt cctgtggctg caaggtgata ttgaggctgg tcgtgacggc
     7261 ttgccattgt tgagtaagtg cttgctcatc ttgtcggagg ctttgcgctt cgttttcatc
     7321 acgctgaagc tgctttgtta aggcgtccag ttgcccacgt agcgtcgcac cttcttcacc
     7381 gagcttttta acttcgtttt ccagcgccag taatcgagac tgattaacac caggctccag
     7441 cgcctgatac gcctcgaccg ccgggtggct ggtggaacca caaagtgggc aaggctgacc
     7501 cgcctgtaac tgtgcacgtt gagcttccag cgttttgatg cgcgcttcct gctcgcaaat
     7561 ggttttgaca tcggcaagtt gctgcgtctt ttctttataa cgctggcgca tttcgttaag
     7621 tgcggcatta cgttgcgtct gttccagagt gacattctgg atagtgacca ttaactgcgc
     7681 cagacgtttt tgttggggaa caatctgccc atgcagcgcg accaggcgct gacgcagtgg
     7741 gcgttgctcg gcatgttgcg ccagggcgct agcaacttca tcggcggtta acgtcaacgt
     7801 gatcgccgca agcgtattaa gtttttgctc agcatgggtt aactgttgct gccattgccg
     7861 cagatgctcg cgatcgctgg tttgttggga gaactgcgca cgccaacccg ccagttcgtt
     7921 gttccactga cggaagcggt cgtgttcctg taaccaggta ttcaggcttt gttgctgctg
     7981 ctgtaattct gctgactgct tcgccgcgtg gtggcgaatg ctcgcgcgaa gcgccattgt
     8041 gctctgtaag cgagtattta cttcttcaat ctgctggcga gtatgcgcca gcgccgcgct
     8101 gtgttctgcg atgcgttccc agtgcggacg aagatttcgt gccggttgtg ccagactgag
     8161 cgccgccagt tgaggttgcg ctttttcttc ttcggctaac gcctgttgca aggcctgttg
     8221 acggcggctg gcttcttgct gcaattcgtc cagacgcgtt aaccagttta gtgattgttg
     8281 ttcttgctgc tgcgcggtaa gtaactgttt ttcttcgtca gtaagtacct gcaaactcgc
     8341 tgtcagcgat tgcacttgtt ctggcgtgag caacgcgacg ccgctggcct gcgcttgcag
     8401 cttctccagc tcagtgcggg ccgatttgtg ctgctcaaaa accatcgccg agatttgccc
     8461 gtagatttca gtgccagtta actcctcgag caattccgcg cgttctttgg gtttggcatt
     8521 caggaaggca gcaaattgcc cctgcgaaag cagcatcgaa cgggtgaagc gcccgtaatc
     8581 cagtccggtt aacgtcgccg tcagttccag cttatctttc actttgtcgg cgagaatttt
     8641 gccatcggcg cagcgcgcca gctctacgcg tggcacctgc aaattaccgt ccggctggtt
     8701 acgcgcccga ttctggctcc agaacgcgcg atacgcttca cctttcactt caaactccac
     8761 ctccgccaga cattcggcgg tatcgcgggt catgagatca ttttgcgatt gtgaaacgtt
     8821 agagaggcgt ggagtttcgt gatacagcgc cagacaaatg gcgtccagca gggtggtttt
     8881 ccccgcaccg gttgggccgg taatggcaaa cagcccgttg ctggcgaacg gctcgcgggt
     8941 gaaatcaatc ttccattcgc cttttaatga gttcaggttt ttcaggcgca ggctgagaat
     9001 tttcatgctt cgtgttctcc ggcgagggta tgcaacgtcg tggtgaaaag atgctgcaga
     9061 cgttgctgct gcgattcctc cagttcttcc agtgccagac ggcgattgaa cacctcttcg
     9121 acgctgagtt cgctgagggt ttcacgctgt tggctggcta acacacgctc gcgctgttca
     9181 cgactccggc gtaccagcaa tacttcaaca ggcaatgatt cggttaatgc ctggattttg
     9241 cgctgaatat catgcagata ctcatcagta gtgatttcga tatccagcca gacaggcggc
     9301 tcctgtgata catcgcgcca ctgttccagc tgtgcggtaa tcgacgccag atcgcctttc
     9361 agaaccgcca tgggttgcgt taccggtacg ttcaggtttt ccacgctctc taatttgccg
     9421 tttgaaaatg tcaccagatg gacatattta ctctttccgc attcatcaaa actcagtgga
     9481 atgggggagc cgcaatagcg aacatgttcc atgccgccaa taatctgtgc gcggtgaata
     9541 tgcccgagcg cgatgtagtc ggctggtgga aagttttgtg ccggaaacgc gtccagcgtg
     9601 ccaatataaa tgtcacgcac ggcgtcactt ttactggccc ccacggtcgt taaatgtccc
     9661 gtggcgatga tgggcagagg ctgatcgccg cgcagtttgc aggcatcggc atagtgttgt
     9721 tggtaataat cggtaatcgc tgccagtaaa tgctgctgtt tttcaatacc gttaagcccc
     9781 gcctggctgg taataatgtc acgtggacgt aaaaacggaa tggggcacag cacggcgcct
     9841 ggcgtcccgt cgcgacgagg caagatttgc ggcgcatgtc cggcgctggc gaccacggtc
     9901 gtattgagga acgccatgat atcgcgcgat tcattcagcg tggcgaccga gtcatggttt
     9961 cctgccagta ccaccagatg acagccagtt tgctgtaaat tgacaacaaa acggttgtat
    10021 aacgtgcggg cgtaactggg cggcgagccg gtatcgaaaa catcaccagc aacaataatc
    10081 gcatccacct gatgggtttg tgctgtttcc agcagccagt caagaaaagc ctgatgttca
    10141 gcttcgcggc ttttactgta gaagttctgg ccgagatgcc agtctgaggt gtgaagaatg
    10201 cgcataacgg ttccctggcg aaaaagcatg ggcgcgatta tacccaaaca gatgtgccat
    10261 ttgctttttt ctgcgccacg gaaatcaata acctgaagag atgtgcgacg agcttttcat
    10321 aaatctgtca taaatctgac gcataatgac gtcgcattaa tgatcgcaac ctatttatta
    10381 caacagggca aatcatggcg agacgtattc tggtcgtaga agatgaagct ccaattcgcg
    10441 aaatggtctg cttcgtgctc gaacaaaatg gctttcagcc ggtcgaagcg gaagattatg
    10501 acagtgctgt gaatcaactg aatgaaccct ggccggattt aattctcctt gactggatgt
    10561 tacctggcgg ctccggtatc cagttcatca aacacctcaa gcgcgagtcg atgacccggg
    10621 atattccagt ggtgatgttg accgccagag gggaagaaga agatcgcgtg cgcggccttg
    10681 aaaccggcgc ggatgactat atcaccaagc cgttttcgcc gaaggagctg gtggcgcgaa
    10741 tcaaagcggt aatgcgccgt atttcgccaa tggcggtgga agaggtgatt gagatgcagg
    10801 gattaagtct cgacccgaca tctcaccggg tgatggcggg cgaagagccg ctggagatgg
    10861 ggccgacaga atttaaactg ctgcactttt ttatgacgca tcctgaacgc gtgtacagcc
    10921 gcgagcagct gttaaaccac gtctggggaa ctaacgtgta tgtggaagac cgcacggtcg
    10981 atgtccacat tcgtcgcctg cgtaaagcac tggagcccgg cgggcatgac cgcatggtgc
    11041 agaccgtgcg cggtacagga tatcgttttt caacccgctt ttaacgcctt gctcatcgga
    11101 cgcagagcag ggcttatgat ttcttaactg gagtatctta cgtgctggaa cggctgtcgt
    11161 ggaaaaggct ggtgctggag ctgctacttt gctgcctccc ggctttcatc ctgggtgcat
    11221 tttttggtta cctgccctgg tttttgctgg catcggtaac gggactgctt atctggcatt
    11281 tctggaattt attgcgcctt tcatggtggc tgtgggtgga tcgtagtatg accccgccac
    11341 cggggcgtgg tagctgggaa ccgctactat acggcttaca ccagatgcag ctgcgaaata
    11401 aaaaacgccg ccgtgaactg ggcaatctga ttaaacgctt tcgtagcggc gcggagtcgc
    11461 tgcccgacgc agtggtgctg accacggaag agggcggtat tttctggtgt aacggtctgg
    11521 cgcaacagat tcttggtttg cgctggccgg aagataacgg gcagaacatc cttaacctgc
    11581 tgcgttaccc ggagtttacg caatatctga aaacgcgtga tttttctcgc ccgctcaatc
    11641 tggtgctcaa caccgggcgg catctggaaa ttcgcgtcat gccttatacc cacaaacagt
    11701 tgctgatggt ggcgcgtgat gtcacgcaaa tgcatcaact ggaaggggcg cggcgcaact
    11761 ttttcgccaa cgtaagccat gagttacgca cgccattgac cgtgttacag ggttacctgg
    11821 agatgatgga tgagcagccg ctggaaggcg cggtacgcga aaaagcgttg cacaccatgc
    11881 gcgagcagac ccagcggatg gaagggttgg tgaagcaatt gctgacgctg tcgaaaatag
    11941 aagccgcacc gacgcatttg ctcaatgaaa aggttgatgt gccgatgatg ctgcgcgttg
    12001 ttgagcgcga ggctcagact ctgagtcaga aaaaacagac atttaccttt gagatagata
    12061 acggcctcaa ggtgtctggc aacgaagatc agctacgcag tgcgatttcg aacctggtct
    12121 ataacgccgt gaatcatacg ccggaaggca cgcatatcac cgtacgctgg cagcgagtgc
    12181 cgcacggtgc cgaatttagc gttgaagata acggaccggg cattgcaccg gagcatattc
    12241 cgcgcctgac cgagcgtttt tatcgcgttg ataaagcgcg ttcccggcaa accggcggta
    12301 gcggattagg gttagcgatc gtgaaacatg cggtgaatca tcacgaaagt cgcctgaata
    12361 ttgagagtac agtaggaaaa ggaacacgtt tcagctttgt tatcccggaa cgtttaattg
    12421 ccaaaaacag cgattaatcc gcctttgtca tcttttattg ccataagcca gccgatgttg
    12481 tcttattttc tttgcagtca aaatacgggc gttagatttt acaacggttg gtgatttttt
    12541 gttcgcatga ttagccatgt ctttttcacg gaaatagtgt tttatactag ttggtgattt
    12601 cttatcgcta tatacctctg gtttttagat ccctccttgc tttaaaacgt tataagcgtt
    12661 taaattgcgc ttcaggtgct gtcatactga ctgcattaac gcggtaaatc gaaaaactat
    12721 tcttcgccgc gcctggttgg gagtatttcc cgctaaaatt gtttaaatat accgctgtat
    12781 catccccagg gattggcaca aaaatttaac gttacaacac cacatccaca ggcagtatga
    12841 tttatgaccc atcaattaag atcgcgcgat atcatcgctc tgggctttat gacatttgcg
    12901 ttgttcgtcg gcgcaggtaa cattattttc cctccaatgg tcggcttgca ggcaggcgaa
    12961 cacgtctgga ctgcggcatt cggcttcctc attactgccg ttggcctgcc ggtattaacg
    13021 gtagtggcgc tggcaaaagt tggcggcggt gttgacagcc tcagcacgcc aatcggtaaa
    13081 gtcgctggcg tactgctggc aacggtgtgt tacctggcgg tggggccgct tttcgctacg
    13141 ccgcgtacag ctaccgtttc ttttgaagtg gggattgcgc cgctgacggg tgattccgca
    13201 ctgccgctgt ttatctacag cctggtctat ttcgctatcg ttattctggt ttcgctctat
    13261 ccgggcaagc tgctggatac cgtgggcaac ttccttgcac cgctgaaaat tatcgcgctg
    13321 gtcatcctgt ctgttgccgc gattgtctgg ccggcgggtt ctatcagcac ggcgactgag
    13381 gcttatcaaa acgctgcgtt ttctaacggc ttcgttaacg gctatctgac catggatacg
    13441 ctgggcgcaa tggtgtttgg tatcgttatt gttaacgcgg cgcgttctcg tggcgttact
    13501 gaagcgcgtc tgctgacccg ttataccgtc tgggctggcc tgatggcggg tgttggtctg
    13561 actctgctgt acctggcgct gttccgtctg ggttcagaca gcgcgtcgct ggtcgatcag
    13621 tctgcaaacg gcgctgctat tctgcatgct tacgttcagc acacctttgg cggcggcggt
    13681 agcttcctgc tggcggcgtt aatcttcatc gcctgcctgg taacggcagt tggcctgacc
    13741 tgtgcttgtg cagaattctt tgcccagtac gtaccgctct cttatcgtac gctggtgttt
    13801 atcctcggcg gcttctcgat ggtggtttct aacctcggct taagccagct gatccagatc
    13861 tccgtaccgg tgctgaccgc tatttatccg ccgtgtatcg cactggttgt attaagtttt
    13921 acacgctcat ggtggcataa ttcgtcccgc gtgattgctc cgccgatgtt tatcagcctg
    13981 ctttttggta ttctcgacgg gatcaaagca tctgcattca gcgatatctt accgtcctgg
    14041 gcgcagcgtt taccgctggc cgaacaaggt ctggcgtggt taatgccaac agtggtgatg
    14101 gtggttctgg ccattatctg ggatcgcgcg gcaggtcgtc aggtgacctc cagcgctcac
    14161 taaatcactg aacatttgtt ttaaccacgg ggctgcgatg ccccgtggtt ttttattgtg
    14221 ttgatgggtt aggaattgat ggaaagtaag aacaagctaa agcgtgggct aagtacccga
    14281 cacatacgct ttatggcact gggttcagca attggcaccg ggctgtttta cggttcggca
    14341 gacgccatca aaatggccgg tccgagcgtg ttgttggcct atattatcgg tggtatcgcg
    14401 gcgtatatca ttatgcgtgc gctgggggaa atgtcggtac ataacccggc cgccagctct
    14461 ttctcgcgtt atgcgcagga aaacctcggc ccgctggcag gttacattac cggttggacc
    14521 tactgctttg aaatccttat tgtcgccatc gccgatgtga ccgcttttgg tatctatatg
    14581 ggtgtctggt tcccgacggt gccgcactgg atttgggtac tgagcgtggt gctgatcatt
    14641 tgcgccgtaa acctgatgag cgtgaaggta ttcggtgagc tggaattctg gttctcgttc
    14701 tttaaagtcg ccaccatcat catcatgatt gtcgccggtt tcggcatcat catctggggg
    14761 attggcaacg gcgggcaacc gaccggtatt cataacctgt ggagcaacgg cggcttcttc
    14821 agtaacggct ggcttggcat ggtaatgtcg ttgcaaatgg tgatgtttgc ttacggtggg
    14881 atcgaaatta tcgggattac cgccggtgaa gcgaaagatc ctgagaaatc tattccgcgt
    14941 gcgattaact ccgtgccgat gcgtattctg gtgttctatg ttggtacact gttcgtcatt
    15001 atgtctatct acccgtggaa tcaggttggc actgccggta gcccgttcgt gctgacgttc
    15061 cagcatatgg gcattacctt tgccgccagc attcttaact ttgttgtgct gactgcttcg
    15121 ctgtcggcaa ttaacagtga cgtatttggc gtaggccgta tgctccacgg tatggcagag
    15181 cagggcagcg caccgaaaat tttcagcaaa acgtcgcgtc gcggtattcc gtgggttacg
    15241 gtgctggtga tgactaccgc gctgctgttt gcggtgtatc tgaactacat catgccggaa
    15301 aacgtcttcc tggtgatcgc ttcgctggca accttcgcca cggtatgggt gtggattatg
    15361 atcctgctgt cgcaaatcgc cttccgtcgc cgtttgccgc cagaagaagt taaggcgctg
    15421 aaatttaaag tgccgggtgg ggtagcaacg accatcggcg ggctgatttt cctgctcttt
    15481 attatcgggt tgattggtta tcacccggat acgcgtatct cgctgtatgt cggtttcgcg
    15541 tggattgttg tgctgttgat tggctggatg tttaaacgcc gccacgatcg tcagctggct
    15601 gaaaaccagt aatacttgcg ttctgtaagc cggttcagga ggaaatcctg atccggctat
    15661 tccgaaagtt atccgccccc gtcctcctcc cccaaatatc cttcagatga tgagtgatcc
    15721 tgcattaggc tatggcaagg tgatcagatt ttcatcacag gggaattatg atgttaaatg
    15781 catggcacct gccggtgccc ccatttgtta aacaaagcaa agatcaactg ctcattacac
    15841 tgtggctgac gggcgaagac ccaccgcagc gcattatgct gcgtacagaa cacgataacg
    15901 aagaaatgtc agtaccgatg cataagcagc gcagtcagcc gcagccaggc gtcacggcat
    15961 ggcgggcggc gatcgatctc tccagcggac aaccccggcg gcgttatagt ttcaaattgc
    16021 tttggcacga tcgccagcgt tggtttacac cgcaaggctt cagccgaatg ccgccagcac
    16081 gactggagca gtttgccatc gatgtaccgg atatcggttc acaatgggct gcggatcaga
    16141 ttttttatca gatcttccct gatcgtttcg cccgtagtct tcctcgtgaa gctgaacagg
    16201 atcatgtcta ttaccatcac gcagccggac aagagatcat attgcgtgac tgggatgaac
    16261 cggtcacggc gcaggcgggc ggatcaacgt tctatggcgg cgatctggac gggataagcg
    16321 aaaaactgcc gtatctgaaa aaacttggcg tgacggcgtt gtatctcaat ccggtgttta
    16381 aagctcccag cgtacataaa tacgataccg aggattatcg ccatgtcgat ccgcagtttg
    16441 gcggtgatgg ggcgttgctg cgtttgcgac acaatacgca gcagctggga atgcgtctgg
    16501 tgctggacgg cgtgtttaac cacagtggcg attcccatgc ctggtttgac aggcacaatc
    16561 gtggcacggg gggagcttgt cacaaccccg aatcgccttg gcgcgactgg tactcgttta
    16621 gtgatgatgg cacggcgctc gactggcttg gctatgccag cttgccgaag ctggattatc
    16681 agtcggaaag tctggtgaat gaaatttatc gcggggaaga cagtattgtc cgccactggc
    16741 tgaaagcgcc gtggaatatg gacggctggc ggctggatgt ggtgcatatg ctgggggaag
    16801 cgggtggggc gcgcaataat ttgcagcacg ttgctgggat caccgaagcg gcgaaagaaa
    16861 cccagccgga agcgtatata gttggagagc attttggcga tgcacggcaa tggttacagg
    16921 ccgatgtgga agatgccgcc atgaactatc gcggcttcac attcccgttg tggggatttc
    16981 ttgccaatac cgacatctct tacgatccgc agcaaattga cgcccaaacc tgtatggcct
    17041 ggatggataa ttaccgcgca gggctttctc atcaacagca attacgtatg tttaatcagc
    17101 tcgacagcca cgatactgcg cgatttaaaa cgctgctcgg tcgggatgtt gcgcgcctgc
    17161 ccctggcggt ggtctggctg ttcacctggc ctggtgtacc gtgcatttat tacggtgatg
    17221 aagtgggact ggatggtaaa aacgatccgt tttgccgtaa accgttcccc tggcaggtgg
    17281 aaaagcagga tacggcgtta ttcgcgctgt accagcgaat gattgtgctg cgtaagaaaa
    17341 gtcaggcgct gcgtcgtggc ggctgtcagg tactgtatgc ggaagataac gtggtggtat
    17401 ttgtccgcgt gctaaatcag cagcgtgtac tggtggcaat caaccgtggc gaggcctgtg
    17461 aagtggtgct ccccgcgtca ccgtttctta atgtcgcgca atggcaacgc aaagaaggcc
    17521 atgggcaact gacagacggg attctggctt tgcctgccat ttcggctacg gtgtggatga
    17581 actaaagcat ctgttttatt tgttacgtcg ttagtcacca gccctgacac tacgcagcgc
    17641 aaaaaagcgg ctcaatgtca gggctgaatt tgttaaatat ttgtcccgat cgatatgagc
    17701 gatttttggt attctttgcc catgatgtta tgtacaacat gctccaggat aaccctgtag
    17761 tggagcaaag atatgacaat ttcaaattcg aatttattga aaccgtttat tactggtgct
    17821 gagttgccac cttttaagga ggatgtcttt tctaaaacag aagaacaatt taaaaaaagc
    17881 acccatggca aatcgctttt catttgcgga aatgaaaact tagtaaacat ttcagcggaa
    17941 aaaatcgatg aaataaaaaa tagcaaaaat ttcttttcaa acgttgtaaa tataataatt
    18001 ttactcatta agtatcctga taaaattctg cccattcttc atggttgtcc tgcagagtcc
    18061 atcgaactca ttaagaatgc gaaacgaacg gggcaatggt catatgagat gactgataag
    18121 aacgggcaga atattgaagt gtcct
//
LOCUS       DAZGBC010000097        17789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGBC010000097 DAZGBC010000000
VERSION     DAZGBC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.097"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1319
                     /gene="dcuC"
                     /locus_tag="ACHK1J_003187"
     CDS             <1..1319
                     /gene="dcuC"
                     /locus_tag="ACHK1J_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7912422.1"
                     /translation="GYSATGVLFVGGLLLLIISAIMGHKVLPSSQASTGYSATDIVEY
                     VKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQYINSPYLLMIAAYF
                     VACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPAAIILAPTSGDVVL
                     AAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHISHEMLDVSEITTT
                     APAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEFLRSFNTQKVFSGL
                     EVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFGSASIILMLVLVIL
                     TMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASNLGRTLSPVSGVVV
                     AVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTGK"
     gene            complement(1359..2039)
                     /gene="dpiA"
                     /locus_tag="ACHK1J_003188"
                     /gene_synonym="citB"
     CDS             complement(1359..2039)
                     /gene="dpiA"
                     /locus_tag="ACHK1J_003188"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO7912423.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2008..3666)
                     /gene="dpiB"
                     /locus_tag="ACHK1J_003189"
     CDS             complement(2008..3666)
                     /gene="dpiB"
                     /locus_tag="ACHK1J_003189"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO7912424.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGHPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4046..5104
                     /gene="citC"
                     /locus_tag="ACHK1J_003190"
     CDS             4046..5104
                     /gene="citC"
                     /locus_tag="ACHK1J_003190"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO7912425.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5119..5415
                     /gene="citD"
                     /locus_tag="ACHK1J_003191"
     CDS             5119..5415
                     /gene="citD"
                     /locus_tag="ACHK1J_003191"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO7912426.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5412..6320
                     /gene="citE"
                     /locus_tag="ACHK1J_003192"
     CDS             5412..6320
                     /gene="citE"
                     /locus_tag="ACHK1J_003192"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO7912427.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6331..7863
                     /gene="citF"
                     /locus_tag="ACHK1J_003193"
     CDS             6331..7863
                     /gene="citF"
                     /locus_tag="ACHK1J_003193"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO7912428.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            7867..8418
                     /gene="citX"
                     /locus_tag="ACHK1J_003194"
     CDS             7867..8418
                     /gene="citX"
                     /locus_tag="ACHK1J_003194"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO7912429.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALCALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCAQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8393..9247
                     /gene="citG"
                     /locus_tag="ACHK1J_003195"
     CDS             8393..9247
                     /gene="citG"
                     /locus_tag="ACHK1J_003195"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO7912430.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDMFRATAGVN
                     THKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTNNSQLTAGQ
                     RLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLLLMAINGDT
                     NVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSPGGSADLLI
                     LTWFLAQI"
     gene            9298..10761
                     /gene="citT"
                     /locus_tag="ACHK1J_003196"
     CDS             9298..10761
                     /gene="citT"
                     /locus_tag="ACHK1J_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO7912431.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            10875..11681
                     /gene="rna"
                     /locus_tag="ACHK1J_003197"
     CDS             10875..11681
                     /gene="rna"
                     /locus_tag="ACHK1J_003197"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO7912432.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEMQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            11911..12321
                     /gene="rnk"
                     /locus_tag="ACHK1J_003198"
     CDS             11911..12321
                     /gene="rnk"
                     /locus_tag="ACHK1J_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO7912433.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            12459..12548
                     /gene="yldA"
                     /locus_tag="ACHK1J_003199"
     CDS             12459..12548
                     /gene="yldA"
                     /locus_tag="ACHK1J_003199"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO7912434.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(12552..13790)
                     /gene="ybdR"
                     /locus_tag="ACHK1J_003200"
     CDS             complement(12552..13790)
                     /gene="ybdR"
                     /locus_tag="ACHK1J_003200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO7912435.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            14010..14438
                     /gene="uspG"
                     /locus_tag="ACHK1J_003201"
     CDS             14010..14438
                     /gene="uspG"
                     /locus_tag="ACHK1J_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO7912436.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14559..16124)
                     /gene="ahpF"
                     /locus_tag="ACHK1J_003202"
     CDS             complement(14559..16124)
                     /gene="ahpF"
                     /locus_tag="ACHK1J_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO7912437.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16253..16816)
                     /gene="ahpC"
                     /locus_tag="ACHK1J_003203"
     CDS             complement(16253..16816)
                     /gene="ahpC"
                     /locus_tag="ACHK1J_003203"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO7912438.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17188..>17789
                     /gene="dsbG"
                     /locus_tag="ACHK1J_003204"
     CDS             17188..>17789
                     /gene="dsbG"
                     /locus_tag="ACHK1J_003204"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO7912439.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLKVPANVSTEQMKV"
BASE COUNT         4197 a         4715 c         4425 g         4452 t
ORIGIN      
        1 aagggtattc cgccactggc gtgttatttg tcggtggcct gttattgctg attattagtg
       61 ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac agcgccacgg
      121 atatcgttga atacgttaaa atattgctaa tgagccgcgg cggcgacctc ggcatgatga
      181 ttatgatgct gtgtggcttt gccgcttaca tgacccatat cggcgcgaat gatatggtgg
      241 tcaagctggc gtcaaaacca ttacagtata ttaactcccc ttacctgctg atgattgccg
      301 cctattttgt cgcctgtctg atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt
      361 tgctgatggc aaccctgttt ccggtgatgg taaacgttgg tatcagtcgt ggagcagctg
      421 ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc
      481 tggcggcgca ggcttccgaa atgtcgctga ttgacttcgc cttcaaaacg acgctgccta
      541 tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa cgttatctgg
      601 ataaaaaaga gcacatatct catgaaatgt tagatgtcag tgaaataacc accactgccc
      661 ctgcgtttta tgccattttg ccgttcacgc cgatcatcgg ggtgctgatt tttgacggca
      721 aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg attgcctcca
      781 ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg gaagtggctt
      841 atcgcgggat ggcagatgca tttgcaaacg tggtgatgct gctggttgcc gctggggtat
      901 tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc gctacctcgt
      961 ttggttcggc gagtatcatc ctgatgctgg tattggtgat tctgacaatg ctggcggcag
     1021 tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg
     1081 cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc
     1141 tcggtcgtac cctgtcaccc gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa
     1201 tctcaccatt tgaagtcgta aaacgcacct cggtgccagt gcttgttggg ctggtgattg
     1261 ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca ggcaagtaaa
     1321 tagtcatgcc ggaggcagtt tcctccggct ttatcatgtc acccactgtg gtatatgcgt
     1381 tgcggtctgc caactttgcc gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa
     1441 tattcaagat aacgcctggc agtggtgcgg ctgatggtca gtgcctgcgc caccgtttct
     1501 gccgtatgtt gcacacccgg ctctttaaac agttttcgca ccgcgtttag cgtcaagggg
     1561 tcaatgccgg tcggtagctc gtccttaggt tcaccgcgag cataagcatt aaacatctca
     1621 tcaatttgct tctggctggc gctatcaata ctttccagca tatgtttacg ctggcggaaa
     1681 cgggttagcg tttgccccag ccgttcatag gcaattggct taatgagata atcaaataca
     1741 ccacaacgta cggcttcaga caccgtttcc atatcgctgg ctgcagtggt aaacaccacg
     1801 tcgccgggat aatgcgcctg caccagttca tgcagtaaat taatccctct accgtcagga
     1861 agatagttat cgagcaagat tagccccggc ttaaaacgct cgatcatcat tcgggcctgc
     1921 gccaggtttc ccgccagtaa tatctgactg aatccgggaa tgtgacgaat atattccgca
     1981 tgcatctctg ccagcggcgt ttcgtcctca acgatcaata gggttaatgg agctgtcatt
     2041 aggtttcact ttcggaatat agattgaaaa taaggtaccg cagggatcat tatcttcgag
     2101 agtgataaca ccaccgcagc gcgttacgta gctggcaatc aagtacaacc caatgccatg
     2161 ttcaccgggc tcgtcagcac gcgtactgac cccctgctca aatattttgt ctcgtagaga
     2221 ctctggaacg ccgcagccct gatcggcgac ttcaatcacc acatcatcgc cttcatcgct
     2281 gaggaataat tcaacgatct tgtttccttc atcgctacgc aggctggctt cgaaggcgtt
     2341 atcaagtaaa ttgccaacaa tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg
     2401 cgaaagctgg ctaccgggga cgatgatcat ttttagcccc agttcgcggg cgcgctgcac
     2461 tttaccaaaa agcagccccg ccacctggcg atcggcaaac gcctcgcgca ggctgtcaat
     2521 aagctgttgc tgggcctgag actccccctg caccatcgcc agcacgcgat catactcttt
     2581 catctgcaac agaccattga gcgtcgacat ccaattgaga tgctcgtgtc gcaatgtacg
     2641 aaggctctcg acgtattgtt ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc
     2701 tttactacga aagctgataa tggcccccag caaatcatca cctgaacgaa tagcttcccg
     2761 gttggcaata acgctcagac cgttaaagtt cgccaccaca tcctgacgtt tttcatcaat
     2821 ctgttcggta aagaaatcgg caggcctgac cacttctgca atgggtttac ccaaccattg
     2881 ccgtccaggg gaactcagcc ccagcatctt tcttgcgtta cgattgatgg cggtaatgta
     2941 accgtgcgga tccaccgcaa tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg
     3001 acggaccacg cgtgcgattt gctttggctc catgcccatc atctgccgac ggatatgcgc
     3061 ggccaggaac cacgacagca acatcagaat ccctaacagc acgacaaaca cgcctgccat
     3121 cggtaataaa aactcagccc gccagctatc gattttactc accaggtagc caatcgacac
     3181 cacgccgatg acttttccat cgtcatcaaa gattggcgtt ttggcgcgca tcgccatacc
     3241 cattgaccct ttcccggtaa tgaagtagct ctcccctttc tccagcgcgc cctgtttggt
     3301 gaactgcata ggatgaccaa ttttctccgg attaggatgg taaaggcgga tcgagtgccg
     3361 gtcgccaatc accacataat caaaatcggt atctctttgt aatttgttag cgatggtcgc
     3421 cagccgtttg tagtcacgcg tttttactgc gctgatgata ctgtcattag aagcaataat
     3481 tttcgcctga ttcattgcca tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc
     3541 cgtaaaatat tgcgccaggg ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa
     3601 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
     3661 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
     3721 aatcaaaata catagatatt aattataatc gtggcaaatt tcattcatta aatcaattaa
     3781 atcacttaaa cctgcgggtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
     3841 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc aaaatactcc
     3901 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
     3961 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
     4021 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
     4081 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
     4141 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
     4201 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
     4261 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
     4321 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
     4381 gttgaccagc gtaccaggcg tgatggtgct gatggaaaac agcgccacgc gattgaaacg
     4441 ctatgccgaa tcgctgaaaa aatttcgtca tcccgggaac aagattggct gcattgtgat
     4501 gaacgccaat ccctttacga agggtcaccg ttatctgatt caacaggctg cggcacagtg
     4561 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     4621 actggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     4681 cgaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     4741 taaccattgt tacaccgaaa ttgatttgaa gattttccgt cagtacctcg ctcccgcgct
     4801 gggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     4861 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcaccgccca tcgaactggt
     4921 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     4981 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     5041 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     5101 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     5161 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     5221 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     5281 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     5341 ttacgtgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     5401 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     5461 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     5521 atgccctgat gtttgacctc gaagactccg tagcattgcg tgaaaaagac accgcccgcc
     5581 gcatggttta ccacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     5641 tcaacgcgct ggattccgaa tggggtgtca acgacctgga agccgtcgtt cgcggtggtg
     5701 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat atcgaaaaag
     5761 agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     5821 cgattgaatc tccgctgggg attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     5881 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     5941 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     6001 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     6061 aagccgccca catcaagcag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     6121 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccgccgcg
     6181 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     6241 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     6301 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     6361 caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg atcttgccgc tttccagaac
     6421 tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     6481 gaagaagcga ttcgtcgatc aggtttacag gacggcatga cggtttcctt ccatcacgct
     6541 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     6601 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     6661 attcgccagg gcgtggttac ccgcatttat acctccggcc tgcgcggtcc actggcagaa
     6721 gaaatttccc gtggtctgct tgcggaaccg gtgcaaatcc actctcacgg cggtcgcgtg
     6781 catctggtac agagtggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgc
     6841 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     6901 gcaatggtcg atgccgacaa cgcaaaacag gtcgtgatgc tcaccgaaga actgctgcct
     6961 tatccgcata atccggcaag cattgaacaa gatcaggttg atctgatcgt caaagttgat
     7021 cgcgttggcg atgctgcaaa aatcggcgct ggtgcgaccc gtatgaccac taacccgcgc
     7081 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     7141 ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg cggtaacccg tttcctggaa
     7201 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc ttggcggtat taccgcgacg
     7261 atggttgacc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     7321 agccatgctg cgcagtcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     7381 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     7441 gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga ccggctctga cggcgtactg
     7501 cgtggtgctt ccggtggtca ctgcgatacc gcaattgcct ctgcgctttc catcatcgtc
     7561 gcgccgctgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     7621 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     7681 ccggaactgg cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     7741 cgcgaacgcg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     7801 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     7861 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     7921 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagcg ccatcctgtt
     7981 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     8041 cgaattttta atcatggcgt gaccgccttg tgtgccttag ccgcaaaaca gggctggcaa
     8101 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     8161 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     8221 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     8281 tcactgccgc ctcgccgctg cctgttgtgc gcgcaaagcg cagccgtctg cgcgcgtgga
     8341 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     8401 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     8461 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     8521 tcgcattaac tgcggtgcgc ataaagatat ggcgctggcg attcagggct ggttaccccg
     8581 tttcattgaa tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tccacggatt
     8641 acgcccaatt ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac
     8701 gcataaaggc agcatttttt ctttagggct gctatgtgcg gcaattggac gtttgcttca
     8761 gctcaaccag ccggttacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg
     8821 cctgaccgat cgcgaactgc gtaccaataa ttcacaactg acggcaggtc aacggttgta
     8881 ccagcagctt ggccttaccg gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat
     8941 caaccacgcc ttgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc
     9001 attgctcgat accttgctcc tactgatggc gatcaacggc gataccaacg ttgcgtcgcg
     9061 cggtggcgag gggggcctgc gctggctaca gcgcgaggcg caaacattat tgcaaaaagg
     9121 gggcattcga acccccgccg atctcgatta tctccggcag ttcgacaggg agtgtatcga
     9181 acgaaatctc agtccaggcg gcagtgccga cctgctgatc cttacctggt ttttagcaca
     9241 gatttaatta tttaagcact tgataaattt tgaaatatta attttcggag aacccgtatg
     9301 tctttagcaa aagataatat atggaaacta ttggccccac tggtggtgat gggcgtcatg
     9361 tttcttatcc ctgtccccga cggtatgccg ccgcaggcat ggcattactt cgctgtgttt
     9421 gtggcaatga ttgtcggcat gatcctcgag ccaattccgg caacagcgat cagttttatt
     9481 gcggttacta tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct
     9541 gacccagcgt ttaatgcgca aaaacaggcg ctgaaatggg gtctggctgg tttttccagc
     9601 accacggtat ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg
     9661 ttaggtcgtc gcattgccct tttcctggtg aaattcatgg gcaaacgcac gctgacgttg
     9721 ggttatgcga ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg
     9781 cgtaccgggg gtacggtttt tccggtcatt aaaaacctgc cgccgctgtt taaatcattc
     9841 ccgaacgatc cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt
     9901 accagtctga gttcgtccat gtttgtcacc ggtgcggcac caaacgtgct gggtctggag
     9961 ttcgtcagca aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg
    10021 ccggttgggg ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg
    10081 gaaatcacac acagtgaaga agtggcaacc tgggcgggtg atgaactgaa aaccatgggt
    10141 gcgctgacac gcagagagtg gacactgatt ggccttgtat tgctcagctt aggtttgtgg
    10201 gtatttggca gtgaagtcat taatgctact gcggtaggtc tgctggcagt ttcgctaatg
    10261 ctggccctgc acgttgtgcc gtggaaagac attacccgct ataacagcgc atggaacacg
    10321 ctggtcaacc tggcaactct ggttgtgatg gctaacggcc tgactcgttc tggctttatt
    10381 gactggttcg ccggtaccat gagtacgcac ctggaaggat tctcaccaaa cgcaacggtg
    10441 attgtactgg ttctggtgtt ctactttgca cactacctgt ttgccagcct gtctgcgcac
    10501 accgcaacca tgctgccggt tattctggcc gtcggtaaag gtattccggg cgtaccaatg
    10561 gaacaactgt gtatcctgct ggtgctgtct attggtatca tgggctgtct gacgccgtat
    10621 gcaaccggtc ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg
    10681 cgtcttggcg caatcttcgg ggtgatttac atctctatgt tgctgttggt tggctggccg
    10741 attctcgcca tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca
    10801 ggtaagccca cctggcgggc ttttttatga tttaatagat agtattcagt gtatgtatga
    10861 gttccacacc cattatgaaa gcattctggc gtaacgccgc gttgctcgcg gtttctctgc
    10921 ttcccttctc ttctgccaac gccgtagcgt tgcaggcaaa acagtatggc gattttgatc
    10981 gctatgtcct ggccctctcc tggcaaaccg gattttgcca gagtcaacac gatcgaaatc
    11041 gtaacgaacg agatgaatgt cgcctgcaaa ccgaaacgac caacaaagct gattttctga
    11101 ccgtacatgg tctgtggcca ggattgccta aatcggtcgc tgcccgtggt gttgatgaac
    11161 gccgctggat gcgcttcggt tgcgctactc gcccaatccc gaatctacca gaagcgcgcg
    11221 ccagccgaat gtgttcatcg ccggaaaccg ggttatcact ggaaacggcc gctaaactaa
    11281 gtgaagtcat gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac
    11341 acggtgcctg ctttggtttt gatccggacg catacttcgg tacgatggta cgcctgaatc
    11401 aagaaattaa agagagcgaa gccggaaaat tccttgcgga taattacggt aaaacagtga
    11461 gccgccgtga ctttgacgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag
    11521 ttaagctaac gtgccagggt aaccctgcgt atttgactga aatgcagatc tcgatcaaag
    11581 ctgacgccat caacgctccg ctttctgcca actcattctt gccacaacca cacccaggca
    11641 actgtggcaa aacctttgtg attgataaag cgggttatta atcctctgat ttacaaaaca
    11701 cgctaattcg tcttccataa accaaggcga ttaagattta tacataatgc gctgcattga
    11761 ggaattccct gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat
    11821 gtgagttaaa ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga
    11881 cactcgcagt accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac
    11941 ctggatgccg aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc
    12001 gccgacgcgt taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca
    12061 cacgacgtgg tgacaatgaa cagccgggtt aaattccgca atcttagcga tggcgaagtg
    12121 cgtgtgcgca cgctggtgta tccggcaaaa atgaccgata gcaatactca gctttccgtt
    12181 atggctccgg taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa
    12241 cttccgggcg gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct
    12301 gctggcgact acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga
    12361 tgcactcgca tcctctttac gcaaaatcag caatttttcc tggttctcac tgttcacttt
    12421 tgctgcaaaa ttattccgtt tcgtttgtgg gaataaccat ggcagaagca ttttacatcc
    12481 tgataggatt tctgattatg gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc
    12541 actcctgacg atcatattgt tccccccggc atcgcattca ccagacctga aaccgccttc
    12601 tgcgccgcct ctgcgctttg tgcaccgggt accagaatca ccttacggca ctcctcttca
    12661 cgtttttcga aaatctcata tccccgggcg gcctcttcaa acggcatata gtgggtaaca
    12721 atttcttctg gtttcagtaa tcctttctca attaacggta ataattctcc cagccatgcg
    12781 tgaacgtggg tctgtcccat tttaaacgtc aaccctttat caaaggcgtc gccaaacagg
    12841 aaaccgtgaa taaatccagc gtagacgccc ggtacgctaa caatgccgcc acgcctgacc
    12901 gccgcaatac actggcgcaa cgctttaccg ctgctgccct ccagtttcag gttagtcagc
    12961 accgtttccg tggtgctgcc tttcgcttca aaaccgacgg cgtctattac tgcatccacg
    13021 ccccggtgac ctgccgtttg ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa
    13081 ttaatcggga tcgcgccgta gcggtcagcg gcgaaacgca agcggtaggg atgatgatca
    13141 acaacaaaaa tctgttccgc accgagcaac cgtgcacagg cgattgtcaa caatcccaca
    13201 ggaccggcac catagactgc aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc
    13261 tgccatgccg ttggcagaat atcagaaagg aaaagcgctt tatcatctga aagcaaaggc
    13321 ggtactttaa acggccccac attcccttta gggacgcgga catattccgc ctgcccgcca
    13381 ggaacgccac catacaggtg actataacca aacaatgccg cgggagcagg tatctgtttt
    13441 ttattgagcg cagcgccttt acccgcattg gtattttcgc aggcggcata ttgctgcatt
    13501 cgacagaaaa aacagtcgcc acaagcaatg acgaacggaa ttaccacccg gtcgcctttt
    13561 tgcaaatttt ttacgtcctt cccggtttca actacttccc ccataaattc atgaccaaaa
    13621 atatcgccat gtttaacctg gggtattttg cctcgataaa gatggaggtc agagccacag
    13681 atcgccgttg ccgtaatacg cagaataata tcatctgcct gttcaatgcc cggatcggga
    13741 acattttcta cctgaacgtg atgtgggccg tgataagtca atgctttcat gcaacctcca
    13801 gagacagttc aaaaaaagtc agtctttaag ggtagctatc acagcgggaa aacgggcggt
    13861 accgcaggtt ttgggagaat tcctggtgtt cagtgagaat atattaatca cagaaaccgt
    13921 tcgtaaggaa aaagaaccag acaaatatca gggataaata ttataatcat ggcgaatgat
    13981 ttgttcatga ttaacaggga gaaagggtta tgtataagac aatcattatg ccagttgatg
    14041 tatttgaaat ggaattgagc gacaaagctg ttcgccacgc tgaattcctc gcccaggatg
    14101 acggagttat tcatctactt cacgtactac ccgggtcagc cagcctgagc ctgcaccgtt
    14161 ttgccgctga tgtgcgtcgt tttgaagagc atctgcaaca tgaagcagaa gaacgtctgc
    14221 aaacgatggt cagccacttc accatcgatc cttcccgcat taaacaacat gtccgttttg
    14281 gtagcgtgcg ggatgaagtc aatgagttgg cagaagaact gggggctgat gttgtagtta
    14341 tcggttctcg caacccatcg atttcgaccc atctgttagg ttctaacgcc tcgagcgtga
    14401 tccgccacgc caatctgcct gtactggtcg ttcgctaaca gataaaaata tttatgccgc
    14461 cctgcctgga acatcccggc agggcttttt aatgccataa aaaagccgcc aggtttgacc
    14521 ccggcggcta agcaattgca ggtgaatctt acttcttctt atgcagtttt ggtgcgaatc
    14581 aggtagtcaa aagcactcag agaggctttg gcaccttcgc cagtggcgat gatgatctgc
    14641 ttgtacggaa ccgtcgtaca gtcacccgct gcgaacacgc ctttgacgtt ggtttcgcat
    14701 ttcgcatcaa tgataatctc gcccatgcgg ttacgttcga ctgcgccttc cagccagttg
    14761 gtgttcggca gcagaccaat ctggacaaaa ataccggcca gttcgatgtt gtgaatatcg
    14821 ccgctgacac gatctcgata ttccagacca acgactttgc tgccgtcgcc tttcacttcc
    14881 gtggtttgcg cattcagaat aatgtcgacg tttttcaggc tgcgcagttt gtcctgcaga
    14941 acctggtcgg ctttcatttc tggcgcaaat tccagcagcg ttacgtgctc aacgataccc
    15001 gccaggtcga ttgccgcttc cacgccggag ttaccgccgc cgataaccgc tacgcgtttg
    15061 cctttaaaca gcgggccgtc gcagtgcggg cagtaggtca cgcctttagt gcgatactga
    15121 tcttcgcccg gaacgttcat gttgcgccat tttgcaccgg tcgccacgat aatgctgcgt
    15181 gctttcagta ccgcgccaga agctgtttca atctggtgta gaccaccttc aactgctgcc
    15241 gggatcagtt tgctggcgct ctggctgtcg atcacatcaa cgtcgtattc atcaacgtga
    15301 actttcagcg cacctgccag tttctggcct tcggtcttcg gtacagaaat gtagttttcg
    15361 atatcaacgg tatcgaggat ctgaccacca aaacgttcgc ccatcagacc ggtacggatg
    15421 cctttacgtg cggagtaaat tgctgccgct gcacccgccg ggccggaacc aacgattaat
    15481 acgtcataag catcacgctt gttcagctct tctgccgcac gtttttccgc gccagtatca
    15541 attttggcaa cgatttcagt caacgtcatg cggccctgac caaactcttt cccgtttacg
    15601 aacactgccg gaacgcccat cacgttgcga tcggtgattt cgttctggaa ggtgccgccg
    15661 tcaattgcag tgtgcttgat gcgcgggttc aatacgctca tcaggttcag cgcctgcacc
    15721 acgtccgggc agttatggca agagagcgag taataggttt cgaattcaaa atcaccgtca
    15781 atatggcgaa tctgctccag cagagactgc gcttctttcg acggatgacc accggtccac
    15841 agtaacgcca gtaccagcga ggtgaactcg tggcccagcg gggagcctgc aaaacgtggt
    15901 ccctggttag aacctgggtt ggtgatcagg aaagacggct tacgcaccgg caagctgtta
    15961 tcttctttaa aggtgacttt gtctgacagt tctgcgattt cagccaacag ttccttgatt
    16021 tctgccgatt tagcgctgtc atccagcgtg gcaattaact caacaggctt ggtcaatttc
    16081 tcaaggtaag ccttgagttg agttttcata tttgtgtcga gcatgtttat ctcctgggct
    16141 ttaaacatca tcatgcaagc ggcctctgcg gctacctgga tgcagcttgc atcatggtgc
    16201 ggggaagaaa atcgggtgcc gcagcacccg attaattacg cgattgcgga acttagattt
    16261 taccaaccag gtccagagac ggagccagag ttgcttcacc ttctttccat ttagccgggc
    16321 aaacttcacc tgggtgagaa gctacgtact gtgctgcttt gattttacgc agcaggtcag
    16381 acgcgtcacg gccaatgcct tcagcggtaa cttcgattgc ctggatgata ccctgcgggt
    16441 caacaacgaa ggtcgcacgg tcagccagac cttcatcttc acgcatgttg tcgaagttac
    16501 gggtcagggc gccagtcggg tcgccgatca tcgcatattt gattttagcg atggtttcag
    16561 agctgctgtg ccatgctttg tgggtgaagt gagtatcggt agatactgcg tatacgtcta
    16621 cgcccagttt ctgcagttct tcgtagtggt cagcaacgtc acccagttcg gtcgggcata
    16681 cgaaagtaaa gtcagccggg tagaagaaga agacgctcca gcggccttcg gtatcttttt
    16741 cggtgatttc gatgaattcg ccgtttttga atgcctggtt tttaaaaggt ttaattttgg
    16801 tgttaatcaa ggacatctat acttcctccg tgttttcgat gagatgtaag gtaaccaatt
    16861 tttaccgatt cggctaatgc gtttccatta tcaaatcgat aagttttacc ttacaacctt
    16921 cgtaagacaa ctttgtgaac tttgcaccgc cagcggcgat aaaaagtaaa atggccccct
    16981 ttccaggtga ccgtgtaatc tgaactaccc tttggtatct tcagcgcctg tctgcaggct
    17041 gcggtgttgc tcttacaaat gcaacaacga catggattac aacaccctca taaacaaagg
    17101 gcaatcacct gatctaagct cttacctatg acaatgatag gttatgcctt ttactcgact
    17161 tttgcactga ctgaaaagga caaattaatg ttaaaaaaga tacttttact ggctctgctt
    17221 cctgcaatcg ccttcgcaga ggaacttcct gctccagtaa aagcgattga aaaacagggt
    17281 attacgatca tcaaaacgtt cgatgccccc ggaggaatga aaggttatct cggaaagtat
    17341 caggatatgg gcgtcaccat ctacctgact ccagatggta agcacgctat ctctggttac
    17401 atgtacaacg agaaaggtga aaacctgagt aacacactta tcgaaaaaga aatttacgca
    17461 ccagccggac gcgaaatgtg gcaacggatg gaacaatccc actggctcct cgacggtaaa
    17521 aaagacgcgc cggtcattgt ctacgtcttc gccgatccgt tctgcccata ttgtaaacag
    17581 ttctggcagc aggcgcgccc gtgggtagat tctggcaaag tacagttgag aacattgttg
    17641 gttggggtta tcaaaccaga aagtccggcg acagccgcgg caattcttgc ctccaaagat
    17701 cccgcaaaaa cctggcaaga atatgaagcc tctggtggca agcttaagct aaaggtgcct
    17761 gcaaacgtaa gtacagagca aatgaaagt
//
LOCUS       DAZGBC010000098        17712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGBC010000098 DAZGBC010000000
VERSION     DAZGBC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.098"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            278..493
                     /gene="essD"
                     /locus_tag="ACHK1J_003205"
     CDS             278..493
                     /gene="essD"
                     /locus_tag="ACHK1J_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO7912440.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            493..990
                     /gene="rrrD"
                     /locus_tag="ACHK1J_003206"
     CDS             493..990
                     /gene="rrrD"
                     /locus_tag="ACHK1J_003206"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO7912441.1"
                     /translation="MPPSLRKVVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1207..1389
                     /gene="rzoD"
                     /locus_tag="ACHK1J_003207"
     CDS             1207..1389
                     /gene="rzoD"
                     /locus_tag="ACHK1J_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO7912442.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1480..1773)
                     /gene="iss"
                     /locus_tag="ACHK1J_003208"
     CDS             complement(1480..1773)
                     /gene="iss"
                     /locus_tag="ACHK1J_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO7912443.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(2133..2327)
                     /locus_tag="ACHK1J_003209"
     CDS             complement(2133..2327)
                     /locus_tag="ACHK1J_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7912444.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            2716..3261
                     /locus_tag="ACHK1J_003210"
     CDS             2716..3261
                     /locus_tag="ACHK1J_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO7912445.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3236..5161
                     /locus_tag="ACHK1J_003211"
     CDS             3236..5161
                     /locus_tag="ACHK1J_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7912446.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5158..5364
                     /locus_tag="ACHK1J_003212"
     CDS             5158..5364
                     /locus_tag="ACHK1J_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO7912447.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5361..6962
                     /locus_tag="ACHK1J_003213"
     CDS             5361..6962
                     /locus_tag="ACHK1J_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7912448.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6943..8262
                     /locus_tag="ACHK1J_003214"
     CDS             6943..8262
                     /locus_tag="ACHK1J_003214"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7912449.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLAAGEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGIMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATALQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8272..8604
                     /locus_tag="ACHK1J_003215"
     CDS             8272..8604
                     /locus_tag="ACHK1J_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7912450.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8659..9684
                     /locus_tag="ACHK1J_003216"
     CDS             8659..9684
                     /locus_tag="ACHK1J_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7912451.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            9726..10121
                     /locus_tag="ACHK1J_003217"
     CDS             9726..10121
                     /locus_tag="ACHK1J_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7912452.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            10133..10486
                     /locus_tag="ACHK1J_003218"
     CDS             10133..10486
                     /locus_tag="ACHK1J_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7912453.1"
                     /translation="MADFDNQFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10498..11076
                     /locus_tag="ACHK1J_003219"
     CDS             10498..11076
                     /locus_tag="ACHK1J_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7912454.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11073..11468
                     /locus_tag="ACHK1J_003220"
     CDS             11073..11468
                     /locus_tag="ACHK1J_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7912455.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11476..12216
                     /locus_tag="ACHK1J_003221"
     CDS             11476..12216
                     /locus_tag="ACHK1J_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7912456.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            12232..12654
                     /locus_tag="ACHK1J_003222"
     CDS             12232..12654
                     /locus_tag="ACHK1J_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7912457.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            12636..13070
                     /locus_tag="ACHK1J_003223"
     CDS             12636..13070
                     /locus_tag="ACHK1J_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7912458.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13063..15624
                     /locus_tag="ACHK1J_003224"
     CDS             13063..15624
                     /locus_tag="ACHK1J_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7912459.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSVSGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHADLLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMTGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            15621..15950
                     /locus_tag="ACHK1J_003225"
     CDS             15621..15950
                     /locus_tag="ACHK1J_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7912460.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            15950..16648
                     /locus_tag="ACHK1J_003226"
     CDS             15950..16648
                     /locus_tag="ACHK1J_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7912461.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16653..17396
                     /locus_tag="ACHK1J_003227"
     CDS             16653..17396
                     /locus_tag="ACHK1J_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7912462.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            17294..>17712
                     /locus_tag="ACHK1J_003228"
     CDS             17294..>17712
                     /locus_tag="ACHK1J_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7912463.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPSFRQKLNEGWYQVRISGRDAGENELSARLNEPLANGAVIHVVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPVGWLGAAAVSGLYAAGASMIL"
BASE COUNT         4078 a         4414 c         5408 g         3812 t
ORIGIN      
        1 tgtatttgtg gtggtgtttt gaacactcgg tagcattctc ataaatatca ttcagtggtt
       61 tacgtacgta aaaaattggt tatgctgtta agagtggtta cttcgtcaca cagcttaaac
      121 ccgccgtcga gctggttttt ccattttttg agtctcgata ttagctgata actcaatacc
      181 taagttattc actgactccg agtctgttac gtttctgctt ttttgcgata cgttgtattc
      241 cctcaattta cacccgcttt gtctgcgagg tggggttatg aaatccatgg ataagttaac
      301 aacgggtgtc gcctatggca cctcagcagg tagtgccggt tactggtttt tacagctgct
      361 cgataaagtc acgccctcac agtgggcagc aataggtgtg ctgggtagcc tggtatttgg
      421 cctgctgacg tacctgacaa acctttattt caagattaaa gaagataagc gcaaggctgc
      481 gagaggtgaa taatgcctcc atcattacga aaagtcgttg ctgctgctat tggtggcgga
      541 gcaattgcta tagcatcagt gttaatcact ggcccaagtg gtaacgatgg tctggaaggt
      601 gtcagctaca taccatacaa agatattgtt ggtgtatgga ctgtatgtca cgggcataca
      661 ggaaaagaca tcatgctcgg taaaacgtat accaaagcag aatgcaaagc actcttgaat
      721 aaagaccttg ccactgtcgc cagacaaatt aacccgtaca tcaaagtcga tataccggaa
      781 acaacgcgcg gcgctcttta ctcattcgtt tacaacgtgg gtgctggcaa tttcagaaca
      841 tcgacgcttc ttcgcaaaat aaaccagggc gatatcaaag gcgcatgtga tcagctacgt
      901 cgctggacat atgctggcgg taagcaatgg aaaggtctca tgactcgtcg tgagattgag
      961 cgtgaaatct gtttgtgggg tcagcaatga acagagtaac cgcgattatc tccgctctgg
     1021 ttatctgcat catcgtctgc ctgtcatggg ctgttaatca ttaccgtgat aacgccatta
     1081 cctacaaagc ccagcgcgac aaaaatgcca gagaactgaa gctggcgaac gcggcaatta
     1141 ctgacatgca gatgcgtcag cgtgatgttg ctgcgctcga tgcaaaatac acgaaggagt
     1201 tagctgatgc gaaagctgaa aatgatgctc tgcgtgatga tgttgccgct ggtcgtcgtc
     1261 ggttgcacat caaagcagtc tgtcagtcag tgcgtgaagc caccaccgcc tccggcgtgg
     1321 ataatgcagc ctccccccga ctggcagaca ccgctgaacg ggattatttc accctcagag
     1381 agaggctgat cactatgcaa aaacaactgg aaggaaccca gaagtatatt aatgagcagt
     1441 gcagatagag ctgcccatat cgatgggcaa ctcatgcaat tattgtgagc aatacacacg
     1501 cgcttccagc ggagtataaa tgcctaaagt aataaaaccg agcaatccat ttacgaatgt
     1561 ttgctgggtt tctgttttaa caacattttc tgcgccgcca caaattttgg ctgcatcaac
     1621 agttttctcc tgtccaattc ccgaaacgaa gaagtgatgg gtgatggttt cctttggtgt
     1681 tactgctgtc ggtttgtttc caacagtaaa cgtctgttga gcacatcctg taataagcat
     1741 tgccagagcg gcagaaaaca acattttttt catcttatta tcctgcattg ttaaaaacgg
     1801 cagaatccta tgtgacaaca attaaacgat agttaaatgg attgatgaaa attaaaacta
     1861 tataggtgga tgctcagcct attggaggag gggggcactc agaatcctgt ggaatgaaat
     1921 aaaccgctct atctgtccat taccctttta gctgcgctgt atcgtcgccg tattcccgca
     1981 ttaaccatga ccgtagcccg acggggaatt ccttctgcgt gagtgtgcgg gaataatcaa
     2041 aaacgatgca caccgggttt tactgtgctg acagacgcag ggttaccctc atagtcgctt
     2101 ttccggtgcg atggtggaag aaaccgggat gtttattcat catcactttg gattgatgta
     2161 tatgctctct tttctgacgt tagtctccga cggcaggctt caatgaccca ggctgagaaa
     2221 ttcccagacc ctttttgctc aagagcgatg ttaatttgtt caatcatttg gttaggaaag
     2281 cggatgttgc gggttgttgt tctgcgggtt ttgttcttcg ttgacatgag gttgccccgt
     2341 attcagtgtc gctgatttgt attgtctgaa gttgttttta cgttaagttg atgcagatca
     2401 attaatacga tacctgcgtc ataattgatt atttgacgtg gtttgatggc gtagatgcac
     2461 gttgtgacat atagatgata attattatca ttttgcgggt cctttccggc gatccgacag
     2521 gttacggggc ggcgacctcg cgggttttcg ctatttatga aaattttccg gtttaaggcg
     2581 tttccgttct tctttgccgt aacttaatgt ttttatttaa aacaccctct gaaaagaaag
     2641 gaaacgacag gtgctgaaaa cgagcttttt ggcctctgtc gtttcctttc tctgtttttg
     2701 tccgtggaat gaacaatgga agtcaacaaa aagcagctgg ctgacatttt cggcgcgagt
     2761 atccgtacca ttcagaactg gcaggagcag ggaatgcccg ttctgcgggg tggtggcaag
     2821 ggtaatgaag tgctttatga ctctgccgcc gccataaaat ggtatgccga aagggatgct
     2881 gaaattgaga acgaaaagct gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca
     2941 gatctccagc cagggactat tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac
     3001 gcacaggagc tgaaaaatgc cagagactcc gctgaagtgg tggaaaccgc attctgtact
     3061 ttcgtgctgt cgcggatcgc aggtgaaatt gccagtattc tcgacgggat ccccctgtcg
     3121 gtgcagcggc gttttccgga actggaaaac cgacatgttg atttcctgaa acgggatatc
     3181 atcaaagcca tgaacaaagc agccgcgctg gatgaactga taccggggtt gctgagtgaa
     3241 tatatcgaac agtcaggtta acaggctgcg gcattttgtc cgcgccgggc ttcgctcact
     3301 gttcaggccg gagccacaga ccgccgttga atgggcggat gccaattact atctcccaaa
     3361 agaatccgca taccaggaag ggcgctggga aacactgccc tttcagcggg ccatcatgaa
     3421 tgcgatgggc agcgactaca tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta
     3481 ttccaaaatg ctgctgggtg tttatgccta ctttatagag cataagcagc gcaacaccct
     3541 tatctggttg ccgacggatg gtgatgccga gaactttatg aaaacccacg ttgagccgac
     3601 catccgcgat attccgtcgc tgctggcgct ggctccgtgg tatggcaaaa agcaccggga
     3661 taacacgctc actatgaagc gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa
     3721 agcggcaaaa aactaccgtg aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc
     3781 ctttgatgag gatattgaac aggaaggctc tccgacgttc ctgggtgaca agcgtattga
     3841 aggctcggtc tggccaaagt ccatccgtgg ctccacgccc aaagtgagag gcacctgtca
     3901 gattgagcgt gcagccagtg aatccccgca ttttatgcgt tttcatgttg cctgcccgca
     3961 ttgcggggag gagcagtatc ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg
     4021 gacgccggat gacccctcca gcgtgtttta tctctgcgag cataatgcct gcgtcatccg
     4081 ccagcaggag ctggacttta ctgatgcccg ttatatctgc gaaaagaccg ggatctggac
     4141 ccgtgatggc attctctggt tttcgtcatc cggtgaagag attgagccac ctgacagtgt
     4201 gacctttcac atctggacag cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga
     4261 ctggatgaaa acgaaagggg atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg
     4321 tgagacgtgg gaggcgaaaa ttggcgaacg tccggatgct gaagtgatgg cagagcggaa
     4381 agagcattat tcagcgcccg ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc
     4441 ccagctggac cgctacgaaa tgcgcgtatg gggatggggg ccgggtgagg aaagctggct
     4501 gattgaccgg cagattatta tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga
     4561 tgaggccatc aataaaacct atacccgccg gaatggtgca gaaatgtcgg tatcccgtat
     4621 ctgctgggat actggcggga ttgacccgac cattgtgtat gaacgctcga aaaaacatgg
     4681 gctgttccgg gtgatcccca ttaaaggggc atccgtctac ggaaagccgg tggccagcat
     4741 gccacgtaag cgaaacaaaa acggggttta ccttaccgaa atcggtacgg ataccgcgaa
     4801 agagcagatt tataaccgct tcacactgac gccggaaggg gatgaaccgc ttcccggtgc
     4861 cgttcacttc cccaataacc cggatatttt tgatctgacc gaagcgcagc agctgacggc
     4921 tgaagagcag gtcgaaaaat gggtggatgg caggaaaaaa atactgtggg acagcaaaaa
     4981 gcgacgcaat gaggcgctcg actgctttgt ttatgcgctg gcggcgctgc gcatcagtat
     5041 ttcccgctgg cagctggatc tcagtgcgct gctggcgagc ctgcaggaag aggatggtgc
     5101 agcaaccaac aagaaaacac tggcagatta cgcccgtgcc ttatccggag aggatgaatg
     5161 acgcgacagg aagaacttgc cgctgcccgt gcggcactgc atgacctgat gacaggaaaa
     5221 cgggtggcaa cggtacagaa agacggacgg agagtggagt ttacggccac ttccgtgtct
     5281 gacctgaaaa aatacattgc ggagctggaa gtgcagaccg gcatgacaca gcgacgcagg
     5341 ggacctgcag gattttatgt atgaaaacgc ccaccattcc cacccttctt gggccggacg
     5401 gcatgacatc gctgcgcgaa tatgccggtt atcacggtgg tggcagcgga tttggtgggc
     5461 agctgcgggc gtggaaccca ccgggtgaaa gtgtggatgc agccctgttg cccaacttta
     5521 cccgtggcaa tgcccgcgca gacgatctgg tacgcaataa cggctatgct gccaacgcca
     5581 tccagctgca tcaggatcat atcgtcgggt cctttttccg gctaagtcat cgcccaagct
     5641 ggcgttatct tggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
     5701 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
     5761 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
     5821 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
     5881 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
     5941 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
     6001 ggatgccgca gaaatggaca tggatacccc gtgaattacc cggcgggcgc gcctcgttca
     6061 ttcacgtttt tgaacccgtg gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg
     6121 taatggagca gatgaagatg ctcgacacgc tgcagaacac gcagctgcag agcgccattg
     6181 tgaaggcgat gtatgccgcc accattgaaa gtgagctgga tacgcagtca gcgatggatt
     6241 ttattctggg cgcgaacagt caggagcagc gggaaaggct gacgggctgg attggtgaaa
     6301 ttgccgcgta ttacgccgca gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga
     6361 tgccggggga ctcactgaac ctgcagacgg ctcaggatac ggataacggc tactccgtgt
     6421 ttgagcagtc actgttgcgg tatatcgctg ccgggctggg tgtctcgtat gagcagcttt
     6481 cccggaatta cgcccagatg agctactcca cggcacgggc cagtgcgaac gagtcgtggg
     6541 cgtactttat ggggcggcga aaattcgtcg catcccgtca ggcgagccag atgtttctgt
     6601 gctggctgga agaggccatc gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca
     6661 gctttcagga agcccgcagt gcctggggga actgcgactg gataggctcc ggtcgtatgg
     6721 ccatcgatgg tctgaaagaa gttcaggaag cggtgatgct gatagaagcc ggactgagca
     6781 cctacgagaa agagtgcgcg aaacgcggtg acgactatca ggaaattttt gcccagcagg
     6841 tccgtgaaac gatggagcgc cgcgcagccg gtcttaaacc gcccgcctgg gcggctgcgg
     6901 catttgaatc cgggctgcga caatcaacag aggaggagaa gagtgacagc agagctgcgt
     6961 aatctcccgc atattgccag catggctttt aatgagccgc tgatgcttga acccgcctat
     7021 gcgcgggttt tcttttgtgc gcttgcaggc cagcttggga tcagccgcct gacggatgca
     7081 gtatccggcg acagcctggc tgccggagag gcacccgcga cgctggcgtt atccggtgat
     7141 gatgacggac cacgacaggc ccgcagttat caggtcatga acggcatcgc cgtgctgccg
     7201 gtgtccggca cgctggtcag ccggacgcgg gcgctgcagc cgtattcggg aatgaccggt
     7261 tacaacggca ttatcgcccg tctgcaacag gctgccagcg atccgatggt ggacggcatt
     7321 ctgctcgata tggacacgcc aggcggaatg gtggcgggag catttgactg cgctgacatc
     7381 atcgcccgtg tgcgtgacat aaagccggta tgggcgctgg ccaacgacat gaactgcagt
     7441 gcaggtcaac tgcttgccag cgccgcctcc cggcgtctgg tcacgcagac cgcccggaca
     7501 ggctccatcg gcatcatgat ggctcacagt aattacggtg ctgccctgga gaaacagggc
     7561 gtggaaatca cgctgattta cagcggcagc cataaggtgg atggcaaccc ctacagccat
     7621 ctaccggatg atgtccggga aacactgcag tcccggatgg atgcaacccg ccggatgttt
     7681 gcacagaagg tgtcggcata taccggcctg tccgtgcagg ctgtgctgga taccgaggct
     7741 gcagtgtaca gcggtcagga ggccattgat gccggactgg ctgatgaact tgttaacagc
     7801 accgatgcga tcaccgtcat gcgtgatgca ctggatgcac gtaaatcccg tctctcagga
     7861 gggcgaatga ccaaagagac tcaatcaaca actgtttcag ccactgcttt gcaggctgac
     7921 gttactgacg tggtgccagc gacggagggt gaaaacgcca gcgcggcgca gccagacgtg
     7981 aacgcgcaga tcaccgctgc ggttgcggca gaaaacagcc gcattatggg gatcctcaac
     8041 tgtgaggagg ctcacggacg cgaagaacag gcccgcgtgc tggcagaaac ccccggtatg
     8101 accgtggaaa cggcccgccg cattctggcc gcagcaccac agagtgcaca ggcgcgcagt
     8161 gacactgcgc tggatcgtct gatgcagggg gcaccggcac cgctggctgc aggtaacccg
     8221 gcatctgatg ccgttaacga tttgctgaac acaccagtgt aagggatgtt tatgacgagc
     8281 aaagaaacct ttacccatta ccagccgctg ggcaacagtg acccggctca taccgcaacc
     8341 gcgcccggcg gattgagtgc gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc
     8401 acccgtaagc tggttgcgtg ggatggcacc accgacggtg ctgccgttgg cattctggcg
     8461 gttgctgctg accagaccag caccacgctg acgttctaca agtccggcac gttccgttat
     8521 gaggatgtgc tctggccgga ggctgccagc gacgagacga aaaaacggac cgcgtttgcc
     8581 ggaacggcaa tcagcatcgt ttaaccttac ccttcatcac taaaggccgc ctgtgtggct
     8641 ttttttacgg gattttttat gtcgatgtac acaaccgccc agctgctggc ggcaaatgag
     8701 cagaaattta agtttgatcc gctgtttctg cgtctctttt tccgtgagag ctatcccttc
     8761 accacggaga aagtctatct ctcacaaatt ccgggactgg taaacatggc gctgtacgtt
     8821 tcgccgattg tttccggtga ggttatccgt tcccgtggcg gctccacctc tgaatttacg
     8881 ccgggatatg tcaagccgaa gcatgaggtc aatccgcaga tgaccctgcg tcgcctgccg
     8941 gatgaagatc cgcagaatct ggcggacccg gcttaccgcc gccgtcgcat catcatgcag
     9001 aacatgcgtg acgaagagct ggccattgct caggtcgaag agatgcaggc cgtttctgcc
     9061 gtgctcaagg gcaaatacac catgaccggt gaagccttcg atccggttga ggtggatatg
     9121 ggccgcagtg tggcgaacaa catcacgcag tccggcggca cggagtggag caagcgtgac
     9181 aagtccacgt atgacccgac cgacgatatc gaagcctacg cgctgaacgc cagcggcgtg
     9241 gtgaatatca tcgtgtttga cccgaaaggc tgggcgctgt tccgttcctt caaagccgtc
     9301 aaggagaagc tggatacccg tcgcggctct aattccgagc tggagacagc ggtaaaagac
     9361 ctgggcaaag cggtgtccta taaggggatg tatggcgatg tggccatcgt cgtgtattcc
     9421 ggacagtacg tggaaaacgg cgtcaaaaag aacttcctgc cggacaacac gatggtgctg
     9481 gggaacactc atgcacgcgg tctgcgcacc tatggctgta ttcaggatgc ggatgcattg
     9541 agtgagggta ttaatgcgtc tccccgttat ccgaaaaact ggaagacatc cggcgatccg
     9601 gcgcgagagt tcaccatgat tcagtcagca ccgctgatgc tgctggctga tcctgatgag
     9661 ttcgtgtccg ttcaactggc gtaatcatgg cccttcgggg ccattgtctc tctgtggagg
     9721 agtccatgac gaaagatgaa ctgattgccc gtctccggtc gctgggtgag caactgaacc
     9781 gtgatgtcag cctgacgggg acgaaagaag aactggcgct ccgtgtggca gagctggaag
     9841 aggagcttga tgacacggat gacgcagccg gtcaggacac gtctgtcagc ccggaaaatg
     9901 cgctgaccgg acatgaaaat gaggtggtat cagcacagac ggataccgtg actgatacgg
     9961 ctgctctggt cacggttgtg gcactggtga cgctgcatac cgatgcactt cacgccacgc
    10021 gggatgaacc tgtggcattt gtgctgccgg gaacggcgtt ccgtgtctct gccggtgtgg
    10081 cagccgaaat gacagaacgt ggcctggcca gaatgcaata acgggaggcg ctgtggctga
    10141 tttcgataac cagttcgatg ctgccattgc ccgcgccgat gaaacgatac gcgggtacat
    10201 gggaacgtca gccaccatga catccggtga gctgtccggc gcagtaatac gtggtgtttt
    10261 tgatgaccct gaaaatatca gctatgccgg acagggcgtg cgcgttgaag gctccagccc
    10321 gtccctgttt gtccggactg atgatgtgcg gcaactgcgg cgtggagaca cgctgaccat
    10381 cggtgaggaa aacttctgga tagaccgggt ttcgccggat gatggtggaa gctgtcatct
    10441 ctggcttggg cggggcgtac cgcctgccgt taaccgtcgc cgctgaaagg gggatgtatg
    10501 gccataaaag gtcttgagca ggccgttgaa aacctcagcc gtatcagcag aacggcggtg
    10561 cccggtgccg ccgcaatggc cattaaccgc gttgcttcat ccgcgatatc gcagtcggcg
    10621 tcacaggttg cccgtgagac aaaggtacgc cggaaactgg taaaggaaag ggccaggctg
    10681 aaaagggcta cggtcaaaaa tccgcaggcc agaatcaagg ttaaccgggg ggatttgccc
    10741 gtaataaagc tgggtaacgc gcggattgtc ctgtcccgac gcaggcgtcg taaaaagggg
    10801 cagcgttcag ccctgaaagg tggcggcagc gtgcttgtgg tgggtaaccg tcgtattccc
    10861 ggcgcgttta ttcagcaact gaaaaatggc cggtggcatg tcatgcagcg tgtggccggg
    10921 aaaaaccgtt accccattga tgtggtgaaa atcccgatgg cggtgccgct gaccacggcg
    10981 tttaaacaaa atattgagcg gatacggcgt gaacgtcttc cgaaagagct gggctatgcg
    11041 ctgcagcatc aactgaggat ggtaataaag cgatgaaaca tactgaactc cgtgcagccg
    11101 tactggatgc actggagaag catgacaccg gggcgacgtt ttttgatggt cgccccgctg
    11161 tttttgatga ggcggatttt ccggcagttg ccgtttatct caccggcgct gaatacacgg
    11221 gcgaagagct ggacagcgat acctggcagg cggagctgca tatcgaagtt ttcctgcctg
    11281 ctcaggtgcc ggattcagag ctggatgcgt ggatggagtc ccggatttat ccggtgatga
    11341 gtgatatccc ggcactgtca gatttgatca ccagtatggt ggccagcggc tatgactacc
    11401 ggcgcgacga tgatgcgggc ctgtggagtt cagccgatct gacttatgtc attacctatg
    11461 aaatgtgagg acgctatgcc tgtaccaaat cctacaatgc cggtgaaagg tgccgggacc
    11521 acactgtggg tttataaggg gaacggtgac ccttatgcga acccgctttc agacgttgac
    11581 tggtcgcgtc tggctaaagt taaagacctg acgcccggcg aactgaccgc tgagtcctat
    11641 gacgacagct atctcgatga tgaagatgcg gactggactg cgaccgggca ggggcagaaa
    11701 tctgccggag ataccagctt cacgctggcg tggatgcccg gagagcaggg gcagcaggcg
    11761 ctgctggcgt ggtttaatga aggggatacc cgtgcctata aaatccgctt cccgaacggc
    11821 acggtcgatg tgttccgcgg ctgggtcagc agtatcggta aggcggtgac ggcgaaggaa
    11881 gtgatcaccc gtacggtgaa agtcaccaac gtgggacgtc cgtcgatggc agaagatcgc
    11941 agcacggtaa cagcggcaac cggcatgacc gtgacgcctg ccagcacttc ggtggtgaaa
    12001 gggcagagca ccacgctgac cgtggccttc cagccggagg gcgtaaccga caagagcttt
    12061 cgtgcggtgt ctgcggataa aacaaaagcc accgtgtcgg tcagtggtat gaccatcacc
    12121 gtgaacggcg ttgctgcagg caaggtcaac attccggttg tatccggtaa tggtgagttt
    12181 gctgcggttg cagaaattac cgtcaccgcc agttaatccg gagagtcagc gatgttcctg
    12241 aaaaccgaat catttgaaca taacggtgtg accgtcacgc tttctgaact gtcagccctg
    12301 cagcgcattg agcatctcgc cctgatgaaa cggcaggcag aacaggcgga gtcagacagc
    12361 aaccggaagt ttactgtgga agacgccatc agaaccggcg cgtttctggt ggcgatgtcc
    12421 ctgtggcata accatccgca gaagacgcag atgccgtcca tgaatgaagc cgttaaacag
    12481 attgagcagg aagtgcttac cacctggccc acggaggcaa tttctcatgc tgaaaacgtg
    12541 gtgtaccggc tgtctggtat gtatgagttt gtggtgaata atgcccctga acagacagag
    12601 gacgccgggc ccgcagagcc tgtttctgcg ggaaagtgtt cgacggtgag ctgagttttg
    12661 ccctgaaact ggcgcgtgag atggggcgac ccgactggcg tgccatgctt gccgggatgt
    12721 catccacgga gtatgccgac tggcaccgct tttacagtac ccattatttt catgatgttc
    12781 tgctggatat gcacttttcc gggctgacgt acaccgtgct cagcctgttt ttcagcgatc
    12841 cggatatgca tccgctggat ttcagtctgc tgaaccggcg cgaggctgac gaagagcctg
    12901 aagatgatgt gctgatgcag aaagcggcag ggcttgccgg aggtgtccgc tttggcccgg
    12961 acgggaatga agttatcccc gcttccccgg atgtggcgga catgacggag gatgacgtaa
    13021 tgctgatgac agtatcagaa gggatcgcag gaggagtccg gtatggctga accggtaggc
    13081 gatctggtcg ttgatttaag tctggatgcg gccagatttg acgagcagat ggccagagtc
    13141 aggcgtcatt tttccggtac ggaaagtgat gcgaaaaaaa cagcggcagt cgttgaacag
    13201 tcgctgagcc gacaggcgct ggctgcacag aaagcgggga tttccgtcgg gcagtataaa
    13261 gccgccatgc gtatgctgcc tgcacagttc accgacgtgg ccacgcagct tgcaggcggg
    13321 caaagtccgt ggctgatcct gctgcaacag ggtggtcagg ttaaggactc cttcggcggg
    13381 atgatcccca tgttcagggg gcttgccggt gcgatcaccc tgccgatggt gggggccacc
    13441 tcgctggcgg tggcgaccgg tgcgctggcg tatgcctggt atcagggcaa ctcaaccctg
    13501 tccgatttca acaaaacgct ggtcctttcc ggcaatcagg cgggactgac ggcagatcgt
    13561 atgctggtcc tgtccagagc cgggcaggcg gcagggctga cgtttaacca gaccagcgag
    13621 tcactcagcg cactggtcaa ggcgggggta agcggtgagg ctcagattgc gtccatcagc
    13681 cagagtgtgg cgcgtttctc ctctgtatcc ggcgtggagg tggacaaggt cgctgaagcc
    13741 ttcgggaagc tgaccacaga cccgacgtcg gggctgacgg cgatggcacg ccagttccat
    13801 aacgtgacgg cggagcagat tgcgtatgtt gctcagttgc agcgttccgg cgatgaagcc
    13861 ggggcattgc aggcggcgaa cgaggccgca acgaaagggt ttgatgacca gacccgccgc
    13921 ctgaaagaga acatgggcac gctggagacc tgggcagaca ggactgcgcg ggcattcaaa
    13981 tccatgtggg atgcggtgct ggatattggt cgtcctgata ccgcgcagga gatgctgatt
    14041 aaggcagaga ctgcgtttaa gaaagcagac gacatctgga atctgcgcaa ggatgattat
    14101 tttgttaacg atgaagcgcg ggcgcgttac tgggatgatc gtgaaaaggc ccgtcttgcg
    14161 cttgaagccg cccgaaagaa ggctgagcag cagactcaac aggacaaaaa tgcgcagcag
    14221 cagagcgata ccgaagcgtc acggctgaaa tataccgaag aggcgcagaa ggcttacgaa
    14281 cggctgcaga cgccgctgga gaaatatacc gcccgtcagg aagaactgaa caaggcactg
    14341 aaagacggga aaatcctgca ggcggattac aacacgctga tggcggcggc gaaaaaggat
    14401 tatgaagcga cgctgaaaaa gccgaaacag tccggcgtga aggtgtctgc gggcgatcgt
    14461 caggaagaca gtgctcatgc tgacctgctg acgcttcagg cagaactccg gacgctggag
    14521 aagcatgccg gagcgaatga gaaaatcagc cagcagcgcc gggatttgtg gaaggcggag
    14581 agtcagttcg cggtactgga ggaggcggcg caacgtcgcc agctgtctgc acaggagaaa
    14641 tctctgctgg cgcataaaga tgagacgctg gagtacaaac gccagctggc tgcacttggc
    14701 gacaaggtca cctatcagga gcgcctgaac gcgctggcgc agcaggcgga taaattcgca
    14761 cagcagcaac gggcaaaacg ggccgccatt gatgcgaaaa gccgggggct gactgaccgg
    14821 caggcagaac gggaagccac ggaacagcgc ctgaaggaac agtatggcga taatccgctg
    14881 gcgctgaata acgtcatgtc agagcagaaa aagacctggg cggctgaaga ccagcttcgc
    14941 gggagctgga tgacaggcct gaagtccggc tggagtgagt gggaagagag cgccacggac
    15001 agtatgtcgc aggtaaaaag tgcagccacg cagacctttg atggtattgc acagaatatg
    15061 gcagcgatgc tgaccggcag tgagcagaac tggcgcagct tcacccgttc cgtgctgtcc
    15121 atgatgacag aaattctgct taagcaggca atggtgggga ttgtcgggag tatcggcagc
    15181 gccattggcg gggctgttgg tggcggcgca tccgcgtcag gcggtacagc cattcaggcc
    15241 gctgcggcga aattccattt tgcaaccgga ggattcacag gaaccggcgg caaatatgag
    15301 ccagcgggga ttgttcaccg tggtgaattt gtcttcacga aggaggcaac cagccggatt
    15361 ggcgtgggga atctctaccg gctgatgcgc ggctatgcca ccggtggtta tgtcggtaca
    15421 ccgggcagtc tggctgacag ccggtcgcag gcgtccggga cgtttgagca gaataaccat
    15481 gtggtgatta acaacgacgg cacgaacggg cagataggtc cggctgctct gaaggcggtg
    15541 tatgacatgg cccgcaaggg tgcccgtgat gaaattcaga cacagatgcg tgatggtggc
    15601 ctgttctccg gaggtggacg atgaaaacct tccgctggaa agtgaaaccc ggtatggatg
    15661 tggcttcggc cccttctgta agaaaggtgc gctttggtga tggctattcc cagcgagcgc
    15721 ctgccgggct gaatgccaac ctgaaaacgt acagcgtgac gctttctgtc ccccgtgagg
    15781 aggccacggt actggagtcg tttctggaag agcacggtgg ctggaaatcc tttctgtgga
    15841 cgccgcctta tgagtggcgg cagataaagg tgacctgcgc aaaatggtcg tcgcgggtca
    15901 gtatgctgcg tgttgagttc agcgcagagt ttgaacaggt ggtgaactga tgcaggatat
    15961 ccggcaggaa acattgaatg aatgcacccg tgcggagcag tcggccagcg tggtgctctg
    16021 ggaaatcgat ctgacagagg tcggtggaga acgttatttt ttctgtaatg agcagaacga
    16081 aaaaggtgag ccggtcacct ggcaggggcg acagtatcag ccgtatccca ttcaggggag
    16141 tggttttgaa ctgaatggca aaggcaccag tacgcgcccc acgctgacgg tttctaacct
    16201 gtacggtatg gtcaccggga tggcggaaga tctgcagagt ctggtcggcg gaacggtggt
    16261 ccggcgtaag gtttacgccc gttttctgga tgcggtgaac ttcgtcaacg gaaacagtga
    16321 cgccgatccg gagcaggagg tgatcagccg ctggcgcatc gagcagtgca gcgaactgag
    16381 cgcggtgagt gcctcctttg tactgtccac gccgacggaa acggatggcg ctgtttttcc
    16441 gggacgtatc atgctggcca acacctgcac ctggacctat cgcggtgacg agtgcggtta
    16501 tagcggtccg gctgtcgcgg atgaatatga ccagccgacg tccgatatca cgaaagataa
    16561 atgcagcaaa tgcctgagtg gctgtaagtt tcgcaataac gtcggcaact ttggcggctt
    16621 cctttccatt aacaaacttt cgcagtaatc ccatgacaga gacagaatca gcgattctgg
    16681 cgcacgcccg gcgatgtgcg ccagcggagt cgtgcggctt cgtggtgaga gcgccggagg
    16741 gggaaagata ttttccctgc gtgaatatct ccggtgagcc ggaggcgtat ttccgtatgt
    16801 cgccggaaga ctggctgcag gcagaaatgc agggtgagat tgtggcgctg gtccacagcc
    16861 accccggtgg tctgccctgg ctgagtgagg ccgaccggcg gctgcaggtg cagagtgatt
    16921 tgccgtggtg gctggtctgc cgggggacga ttcataagtt ccgctgtgtg ccgcatctca
    16981 ccgggcggcg ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcttatc
    17041 atctggcggg gattgagatg ccggattttc atcgcgagga tgactggtgg cgtcacggtc
    17101 agaatctcta tctggataat ctggaggcca cagggctgta tcaggtgccg ttgtcagcgg
    17161 cgcagccggg cgatgtgctg ctgtgctgtt ttggttcatc ggtgccgaat catgccgcca
    17221 tttactgtgg tgacggcgag ctgttgcacc atattcctga acaactgagc aaacgagaga
    17281 ggtataccga caaatggcag cgacgcacac actccctctg gcgtcaccgg gcatggcacg
    17341 catctgcctt tacggggatt tacaacgatt tggccgccgc atcgaccttc gtgtgaaaac
    17401 gggggctgaa gccatccggg cgctggccac acagcttccg tcgtttcgcc agaaactgaa
    17461 tgagggctgg tatcaggtgc gcatttccgg gcgtgatgca ggcgaaaatg aattatctgc
    17521 ccgtcttaat gaaccgctgg caaatggtgc cgtgatccac gtcgttccgc gtctggcggg
    17581 tgccaaaagt ggcggtgtgt ttcaggcggt gctgggtgcg gcgctgattg cggtggcatg
    17641 gtggaaccct gttggctggc tgggggccgc ggctgtatcg ggtctgtatg cagcaggggc
    17701 cagtatgatc ct
//
LOCUS       DAZGBC010000099        17438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGBC010000099 DAZGBC010000000
VERSION     DAZGBC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.100"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            158..1369
                     /gene="mhpT"
                     /locus_tag="ACHK1J_003229"
     CDS             158..1369
                     /gene="mhpT"
                     /locus_tag="ACHK1J_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO7912464.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLTSLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            1471..2010
                     /locus_tag="ACHK1J_003230"
     CDS             1471..2010
                     /locus_tag="ACHK1J_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO7912465.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(2236..3069)
                     /gene="fghA"
                     /locus_tag="ACHK1J_003231"
     CDS             complement(2236..3069)
                     /gene="fghA"
                     /locus_tag="ACHK1J_003231"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7912466.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(3162..4271)
                     /gene="frmA"
                     /locus_tag="ACHK1J_003232"
     CDS             complement(3162..4271)
                     /gene="frmA"
                     /locus_tag="ACHK1J_003232"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO7912467.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(4306..4581)
                     /gene="frmR"
                     /locus_tag="ACHK1J_003233"
     CDS             complement(4306..4581)
                     /gene="frmR"
                     /locus_tag="ACHK1J_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO7912468.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(4741..5787)
                     /gene="fbpC"
                     /locus_tag="ACHK1J_003234"
     CDS             complement(4741..5787)
                     /gene="fbpC"
                     /locus_tag="ACHK1J_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO7912469.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADTA"
     gene            complement(5799..7877)
                     /locus_tag="ACHK1J_003235"
     CDS             complement(5799..7877)
                     /locus_tag="ACHK1J_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7912470.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFSEATREQRSRHYFDAGWALLCMAFIVIS
                     ATVVGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(7946..8977)
                     /locus_tag="ACHK1J_003236"
     CDS             complement(7946..8977)
                     /locus_tag="ACHK1J_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7912471.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(8974..10290)
                     /gene="uhpC"
                     /locus_tag="ACHK1J_003237"
     CDS             complement(8974..10290)
                     /gene="uhpC"
                     /locus_tag="ACHK1J_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO7912472.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(10363..11904)
                     /locus_tag="ACHK1J_003238"
     CDS             complement(10363..11904)
                     /locus_tag="ACHK1J_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO7912473.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQIVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMHERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(11904..12533)
                     /locus_tag="ACHK1J_003239"
     CDS             complement(11904..12533)
                     /locus_tag="ACHK1J_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO7912474.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRVQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            12836..13798
                     /gene="tauA"
                     /locus_tag="ACHK1J_003240"
     CDS             12836..13798
                     /gene="tauA"
                     /locus_tag="ACHK1J_003240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7912475.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            13811..14578
                     /gene="tauB"
                     /locus_tag="ACHK1J_003241"
     CDS             13811..14578
                     /gene="tauB"
                     /locus_tag="ACHK1J_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO7912476.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            14575..15402
                     /gene="tauC"
                     /locus_tag="ACHK1J_003242"
     CDS             14575..15402
                     /gene="tauC"
                     /locus_tag="ACHK1J_003242"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO7912477.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            15399..16250
                     /gene="tauD"
                     /locus_tag="ACHK1J_003243"
     CDS             15399..16250
                     /gene="tauD"
                     /locus_tag="ACHK1J_003243"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO7912478.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPLQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETLPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(16357..17331)
                     /gene="hemB"
                     /locus_tag="ACHK1J_003244"
     CDS             complement(16357..17331)
                     /gene="hemB"
                     /locus_tag="ACHK1J_003244"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO7912479.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         4138 a         4638 c         4755 g         3907 t
ORIGIN      
        1 aagcgtcgca tcaggcatct gcacacgact gccggatgcg gcgtgaacgc cttatccggc
       61 ctacatttcg cccgtaggca gtcattaaat agttctgatt acgcggctgg caatgtatca
      121 gtcgcgattc acctgcactc gcaacgaggt tcatcttatg tcgactcgta ccccttcatc
      181 atcttcatcc cgcctgatgc tgaccatcgg gctttgtttt ttggtcgctc tgatggaagg
      241 gctggatctt caggcggctg gcattgcggc gggtggcatc gcccaggctt tcgcactcga
      301 taaaatgcaa atgggctgga tattcagcgc cggaatactc ggtttactac ccggcgcgtt
      361 ggttggcgga atgctggcgg accgttatgg tcgcaagcgc attttgattg gctcagttgc
      421 gctgtttggt ttgttctcgc tggcaacggc gattgcctgg gatttcccct cactggtctt
      481 tgcgcggctg atgaccggtg tcgggctggg ggcggcgttg ccgaatctta tcgccctgac
      541 gtctgaagcc gcgggtccac gttttcgtgg gacggcagtg agcctgatgt attgcggtgt
      601 tcccattggc gcggcgctgg cggcgacact gggtttcgcg ggggcaaact tagcatggca
      661 aacggtgttt tgggtaggtg gtgtggtgcc gttgattctg gtgccgctat taatgcgctg
      721 gttgccggag tcggcggttt tcgctggcga aaaacaggct gcgccaccac tgcgtgcgtt
      781 atttgcgcca gaaacggcaa ccgcgacgct gctgctgtgg ctgtgttatt tcttcactct
      841 gctggtggtt tacatgttga tcaactggct accgctgctt ttggtggagc aaggattcca
      901 gccatcgcag gcggcagggg tgatgtttgc actgcaaatg ggtgcggcga gcgggacgtt
      961 aatgttgggc gcattgatgg ataagctgcg tccagtaacc atgtcgctac tgatttatag
     1021 cggcatgtta acttcgctgc tggcgcttgg aacggtgtcg tcatttaacg gtatgttgct
     1081 ggccggattt gtcgcggggt tgtttgcgac aggtgggcaa agcgttttgt atgccctggc
     1141 accgttgttt tacagttcgc agatccgcgc aacaggtgtg ggaacagccg tggcggtagg
     1201 gcgtctgggg gctatgagcg gtccgttact ggccgggaaa atgctggcat taggcactgg
     1261 cacggtcggc gtaatggccg cttctgcacc gggtattctt gttgctgggt tggcggtttt
     1321 tattttgatg agtcggagat caagaatgca gccgtgtgcc gatgcctgac gcgccttatt
     1381 aagcaggggc gaaagggact tgcccccaat acacccacgg cgtttacagg tatactcgct
     1441 aaaaattatt cagcgggttt ggaaacaaag atggcaaaac ttaccttaca agagcagttg
     1501 ctcaaagcag gattagtcac cagcaaaaaa gcggcgaagg tcgagagaac ggcgaaaaaa
     1561 tcgcgcgttc aggcgcgtga agctcgggcg gcggtagaag aaaataaaaa ggcacagctt
     1621 gagcgtgata aacagcttag cgaacagcaa aaacaagcgg cgttggcaaa agaatataaa
     1681 gctcaggtga agcagcttat tgaaatgaac cggatcacca ttgccaatgg cgatattggt
     1741 tttaacttca ctgatggcaa tctgattaag aagatttttg tcgataagct cacacaagcg
     1801 cagttgatta atggtcgtct ggcgattgcc cgcttgttgg tcgataacaa tagcgaaggt
     1861 gaatacgcca ttattcccgc cagcgttgcc gataaaattg ctcagcgtga tgccagcagt
     1921 attgtgttgc acagcgcgct cagtgccgaa gagcaggatg aagacgatcc gtatgccgac
     1981 ttcaaagtgc ctgatgattt gatgtggtaa acatagataa gccgtgcgca aatgcccgat
     2041 gcgacgctat gcgcgtctta tcatgcctat ggtttatggg cgaagtgtag gccggataag
     2101 gcgttcacgc cgcatccggc agccgtgcgc agatgcctga tgcgacgctt acgcgtctta
     2161 tcatgcctac ggtttatggg cgaagtgtag gccggataag gcgttcacgc cgcatccggc
     2221 agtcgtgcac tattatcaac gcatattcag tttattggcg tggtaggcaa tatgctcgcc
     2281 aataaaactg gagacaaaat aatagctgtg gtcgtaaccc tcgtgataac ggattaacgt
     2341 cttgatattc atctcctggc agatcttttc aagatttgga gtccgcagct gttctgcgta
     2401 aaaatcatca ctcaacccct gatcaaccat gatttccgca acgcgttgac cttgtgaaat
     2461 aagactcacc ggatcgtaat ccaaccaggc atctttattt tcaccaagat atgcggcaaa
     2521 ggcttgctgt ccccacggta cttgcgatgg ggagacaatg ggcgaaaacg ccgagacgct
     2581 gacatattca tctgggttac gtaacgccag caccagcgcg cccagtccgc ccatagaatg
     2641 accagagata gactttttgg ccgttgccgg aaaatgattc atcactaaat ccggcagctc
     2701 gttgcggata tagtcataca ttttgtaatg ttcattccac ggcgcttgcg tcgcgttcag
     2761 gtaaaacccg gcaccttgcc cgagatcgta acggtcagca tctgcgacat gactgcctcg
     2821 cggactggtg tccggcgcaa caacaataat gttgtgctca gccgcgtaac gctgcatccc
     2881 cgatttagta atgaaattct gctcgttgca ggtcaggcct gaaagccagt acagcaccgg
     2941 caatttttca ttcgcggctt ttggtgggag atagacgccg acattcattt cacatttcag
     3001 tgatgcggaa taatgccgat acacattttg ccagccgcca aagctggcat gtttttcaat
     3061 gagttccatc agtcattatc tcacggttaa tcccccagac ggctgcccgg ggagagtatt
     3121 aagaaccccg tctgaagtgg acggggtaaa ccagcgggaa atcagtaacg aattacggtt
     3181 cgaatggatt tgccttcatg catcaggtcg aaggcgtcat taatttcatc caggctcatg
     3241 gtatgcgtga caaacggttc cagatcgata tcacctttca tcgcatcttc aaccataccc
     3301 ggtaactggg aacgaccttt cacgccgcca aacgcggaac ctttccatac gcgaccagtg
     3361 accaactgga atggacgggt ggagatttcc tgaccggaac ccgcgacccc gatgatcacc
     3421 gactgacccc agccgcggtg cgcactttcc agcgccgcac gcatcacgtt gacattaccg
     3481 atgcattcaa aggtatggtc gataccccat ttgttgatat ccagcaggac atctttaatc
     3541 ggtttgtcgt agtcattcgg gttaatgcag tcggtggcac cgaagcgacg cgccagatcg
     3601 aatttcttcg ggttggtatc gatagcgata atccgtcccg ctttcgcctg acgcgcgccc
     3661 tgaaccactg ccagaccaat cgcgccaaga ccaaatacgg caacagaatc acctggctgg
     3721 actttagctg tgttgtgtac cgcgccaata ccggtggtca cgccacagcc cagcaggcag
     3781 acgtgttcat ggtttgcttc tggattaatt ttggccagag acacttccgc gaccacggtg
     3841 tattcactga atgtagagca ccccatgtag tgataaagcg gctgcccgtt gtaagaaaaa
     3901 cgggtggtgc cgtctggcat caggccttta ccctgggttt cgcgaaccgc aacacagagg
     3961 ttagttttgc cagaacgaca gaactcacac tcgccgcact ctgcggtgta aagcgggatc
     4021 acatggtcgc ctggtttgac gctggttacg ccttcaccga cttcaaccac aacgccagcc
     4081 ccttcgtgac cgagaaccac cgggaataca ccttccgggt catcgccgga gagggtaaat
     4141 gcgtcggtat ggcaaacgcc ggtatgggtg actttaatca gcacttcacc ttttttcggt
     4201 ggtgcaacgt caatttcaac gatttccagc ggtttaccgg gagcaaatgc aacggcagca
     4261 cgtgatttca tctctcgctc ttcctcaata tagtaataga ttcagctatt taagataggc
     4321 acgaaccagt tcaatagtgt cgtcaacgga ttggctgact tcgcggctgt agcagtcatt
     4381 tcggtcaaac gtttcccgga tatggctttc aagcacttct gccatcagcc cattagccgc
     4441 gccccgaacg gcagcgatct gttggagtat ggcacggcat tcggcatcac cctccagcga
     4501 ccgttccaga gcatcaatct gcccccgaat acgacgaact cgagtaagga cctttttctt
     4561 ctcttccgga gtactgggca tttcgcacct catcatctgc acgcaatata ctataggggg
     4621 gtatgctata tgtcaatgca taccccccta tagtatataa cactggagaa taattgttgt
     4681 ccgaagccta accaggtgaa cggtccctca agacagatgg ggaccgttta tgtgctattt
     4741 ttatgccgta tccgctaaaa caaacatccc gtaaggatgg atttcgaggt aatagtgctc
     4801 accaatatcg ggctgtaaac gggtggcgtt tacttgtaat aaaatctcct gtccatgcca
     4861 ctctacggtc acttcgtact gcggccccat gtaggcgaca tggcgaataa cacaacgctg
     4921 actctcttcg ccgtgattgc ttaaggtgat ggcttccggg cgaacgccga cggtgccgct
     4981 accttgcgcc gggaaatgcg ccgcgcgcgg caggcgataa ccgtagatat cgacatactc
     5041 ttcgctaaag ttcgccggga acaggttggc gtcgcccata aaacttgcca taaatcggga
     5101 ggctggctga cgatagagat cctgcggtga gccaatctgc atgatgtgac ctttattcat
     5161 taccagcaca gtgtcggaca ccgcgaaagc ttcactttga tcgtgagtga cgtagagcga
     5221 cgtgatatta aactgctttt gcagctcgcg gatcttatcg cgcatactgc ggcgcaggtt
     5281 ggcatcgagg ttacttaacg gctcatcaaa tagcagcacc tttggcttga ggatcaacgc
     5341 gcgggccagt gccacacgct gttgctgacc accggaaatc tgatcgacat agcggtcctc
     5401 gaagccttcc agatccacca ttgccagcgc ctccttcacc cgctgtttca cttcgctacg
     5461 cgacacgcca agcatcttca gcccgtagcc aacgttttca cccagcgaca tatgcgggaa
     5521 cagagcgtat gactgaaaca ccatgcagat atcgcgctgt tgaatggaac gatgcgtgac
     5581 atcttcgcca tcaataaaaa tttgcccttc gctcggtttt tccagcccgg caaccagacg
     5641 caaaatggtg gttttgccac agccggaagg accgagcagc gtcaccattt gcccttgtgg
     5701 aatggtgaga ttgatattgt cgataaccgt attactgccg aatcgtttag tgacgttgcg
     5761 cagttcaaca aaatttttct gactcatagt gcgctccatt acgcctggtt tttggcttta
     5821 gaacgggaga tacgagcttc tccgatcagc cagtcaaaga ggaaaataat cgccagcatc
     5881 accacgatca gaatcgaacc ataggcgatg gctacgccat attcgccgtc ttcaacgcgg
     5941 ttaagaatgt aggcggttgc cacgcgggtg tccggcgtga cgagaaagac gatggcgctg
     6001 accgtcgtga tggcccgcac aaagctgtag atcagggccg agagaatggc cggacgcagc
     6061 agcggcagta aaatgtgggt tatggttcgc aaggaacccg cgcgcaggct gagtgaggct
     6121 tcatccagcg atttgtcgat ctgccccaga cccgcaatcc cggcgcgaat acccaccgga
     6181 acgttacgca tcaccatcga gataatcaca attgccgccg ttccggtgag ataaaccgga
     6241 gcgctgttaa aggcgagaat atacgagacg cccgccaccg tacccggtac ggcaaagcac
     6301 agcatggtgg tgaactcgat ggtctttttg cctttgaatt gttgacgcac caccacccag
     6361 gcgatgagca ggccaaagat agcggtaatg ggagcggcaa tcccggcgta gagcagggta
     6421 tccagcagtg aaggccacgc gccgtcgctc attccctgac caaatagctt gataaagtta
     6481 tccagcgtca gggtgtagtc cacgccccag ttcaccgtga agctgccgta gaaaatgctg
     6541 ccgtagagca gggcgttaaa ggcaatccat atcgcaagta tggcgaccac gctccacacc
     6601 agggtgaccg gtagcggctg gacgtcgcca cggtaggatt tgccggaaac cgtgacgtaa
     6661 gagcgtttac caatccacat atactggatg cagaacacca gcagcgagaa cagcagcagg
     6721 aaagcgccaa gggtgctggc ggcctgataa tccagctgcg agccggtaat atagaagtaa
     6781 atttgcgtcg ccagtacgtc gaagttaccg ccaagtacca gcgggttact gaaatcagcc
     6841 agtgactgta ccacgacgat taaaaaggca ttggcgagtg cgggcttcaa caacggcaca
     6901 aacacgccgt taaaggtttg ccagcggctg gcgcgcaggg tgtaggaggc ctcttccagc
     6961 gagggatgaa tggttttaat ggccccgtcg aggatcataa atgccattgg cgtgaatgcc
     7021 agaacctgtg ccagccagat cccggtaaaa ccgtacagcc agttggtgtt ggtcagccca
     7081 aaccactcca ccataaattc cgtaacatag ccggagcgcc ccatcatgag cgtcacgcct
     7141 aagccgacca caaacggtgg cgtaacgatg ggcaaaatgg aaaagatgcg gccaataatg
     7201 gcgctgcgtt tagcgatgcg cgtggtataa atcgccagca ccaggccgaa gaaagtacag
     7261 cctatcccca ccgctatcga caagacgata gagttgacga tcacctgaac aatatgtgcc
     7321 tgcgagagca cggtcataaa cgccagcggg gcgaactctc ccgcgtcgtt ggtgaacatc
     7381 gggataaaaa tggcgatgct cggccagacg ataaaaatgc caatcagcgc cacaatcgca
     7441 atcagtgagc cgatcacgaa gcggtcgcca cccagccatt cgaggcgggt taaggccagc
     7501 gtcataatcg cgcctagttc gacaaacagc acgatggcgg cgtagcctaa accgcgtcca
     7561 accacggtgg cgctgatgac gataaacgcc atacagagca atgcccagcc ggcatcgaaa
     7621 taatgacggc tacgttgttc gcgcgtggcc tcactaaacg ggcggaccag caacaggctc
     7681 gggagtaaat accacagcca gctgatatta aaatgcgacc agccgtacgc cccgaggatc
     7741 tcatcactcg tggactccag caggccataa tcgaggctcc agctcggcaa caggacaaat
     7801 gccagccaac ccagcaaaac ccaaaggaaa atcgcatccc gctttttcac gggatggagt
     7861 gcaagtgtct gtgacatagc agttccggta tggttggccg gggcaggcgt ctgcgctgca
     7921 cccggcaaat gcgcctaatg cgggtttatt tacccatttt tacttcgcta acccatttat
     7981 taatcaatgc cttacgaaca tccgtggaac cgtatttgtc catgtcgtag ttgattaatt
     8041 tcaggtcgtc gagcttcagt gagtttggcg acgtttcggc ggtggtattg gtcaggatct
     8101 gataagactt ccctttcttc cacgccagtt cctgcgcttc ttttgataat acccagtcca
     8161 caaacagttt ggcgttgtcg aggttgcgcg cgcctttcag aatgctgacg ccgccaattt
     8221 cgtagcctgt accttcacac ggggaaatca actccagcgg cgcgccctgt tctttttcca
     8281 gcgagtagtc atgcaggaag ccaataccaa tcgccgtttc gccacgggcg gcgttacgtg
     8341 ccggggctat gcctgatttg gtgtactgag agacgttggc gttcagttgt tttagataat
     8401 caaaggcctg atcttccccc cacagctgcg caaaagtagc cagggcggtg taggcagtac
     8461 cggaactttg cggatcggca atttgaattt cgcctttata ttccggtttg gtgagatctt
     8521 tccagcactt cggcaccggc aggtttttct ctttcagacg atcggtgttt acgccaaaac
     8581 cgaggatccc cacataaacg gcagaggaca gattcccttt cacttttgcc ggatcgcgga
     8641 atttctccat gatctgctca aggttagggg atttatacgc ctgtagcagg cccatctcgc
     8701 cagcctgcga ttgcgggtct aaagtgccgc cgtaccagac gtcagcctgg gggtttttct
     8761 tttcagcatc gacttttgcc agggtgcttc cggaaccgtt gcggataaac gaggttttta
     8821 catcatattt ctcgccaaac gctttggttt cggcctcaca catttcattg gtggcgctgc
     8881 aatagaccac cagccggcct tttgcctgcg cggcacccgt taaagtcgcc agcgcaacgg
     8941 cagaagcaat cagagtagag gtaaaggtca ttttcattgt tttatccttc gagcgtgagt
     9001 ggtggtgatg cccgccatca gtaacggcat cagcagtaaa cccataagta ctgcggcgac
     9061 cgagagtaaa ctgaacattc ccggccagcc gtagcgttcg atgaccagcg acagcggcca
     9121 gcctgccagc gccgcgccaa ggtaagcgaa caggccgaga aatccggtga ttgaacccgc
     9181 cgcagctttg tgcccacact ccaccgccgc aagtccaatc agcatttgtg ggccaaagac
     9241 gaaaaagccc acggtaaaaa agcacactgc cagcagagcg tagtgatgaa ccggtgccag
     9301 ccacagtgcg gcgactgaca ccatcaaccc caacgtgaac agcaaaatca ttggcgctcg
     9361 ttgcccgcta aacagcaaat ccgatcccca tccggcaaat aacgcgccga gtaagccgcc
     9421 cacttcaaac agcattaccg tggcgttggc gctgagcagg ttgacaccgt ggctttccgt
     9481 cagccagata ttgccccagt cgttaagcgc aatccggatc acataaacca gtacataaga
     9541 gacgcccagt agccagatca gcggattttg cagcatggtg gtgcgcaaca tttgccacag
     9601 ccccatcggc ggactttgtt gttcctgacg taactccagc ggatcgtgac gccagtgacc
     9661 aactgtcggt aatccctctt cctggggcgt gcctttgagt tgtagcgtta accagatccc
     9721 taatgccatg ctgattatcc ccggtgtcag catcgcggcc tgccagcccc accagtgggc
     9781 ggcaaaggcg ctaatcagcg gaataatcgc tccgccaatg ttgatcgaca tattccagca
     9841 gccccaccag aaaccgcgct cattgcgtga gtaccagtgg gtcagcaagc gggcacacgg
     9901 tggccatccc cagccctgaa aaaaaccgtt cagtgtccag accacaagca gaagcgtaag
     9961 tgattcgcca aaggcaaaca ccacgttcaa taacccggtg gcgaacaagc cgacgcccat
    10021 aaaaccgcgt tgtccgtggc tgtcatgcca cagtccggcg gtaaatttcg acaatccgta
    10081 gctgagataa aacagcgaac ccagcaggcc aatgtcgcct ttatccagtc cgaggtcggt
    10141 ttgtagcgcc ggaaggacgt aattcacgct ttttcgcgtc agataaaatg cggcgtagcc
    10201 aataatcatg cacatcagca atcgcggacg taatgtccgg tagcattgat taatttctct
    10261 cgctgaacgc gcgtgcatgg ttgtctccat gttgctgact atatgaaaat gaaagtgaaa
    10321 ggggatgaga gaaaatccgg gatacctaag actttttcct ggttatccgg cgatttgttg
    10381 caaaaatgtg ggcaagttaa caattacccg cgtgccgtgc tgtttttcga gcgttaagtc
    10441 gccgcccagg gcgctgacgc gctcatgcat cccctgaatg ccaaatccgg ccattttccc
    10501 gctggcgatc cccacaccgt tgtcgctgac ttccagatgc aatacctctc cctgctggcg
    10561 caggataatc gtcacttcgc tggcttcggc gtgtttacag atgttattaa gcaactcctg
    10621 caataaccga tacaaggtaa agcgcaccgt ttcgttttct ggtgtggagt tcagttgata
    10681 ggcaaactgg caatgaatgc cgcgttcact aaaggcaaat tcattaatca gatgcaacaa
    10741 cgcttcgcga aatgtcagtt catcaagcgc gggaggccgc aactggcgca gcaattggcg
    10801 ggtggagaga tgaatgcggc gggcgagctc attgatttgg ctggctgcgg attgaatttg
    10861 cgccggatca cgcgcccgct tcaccagttg cgactggatc tgaatggcgg tgatgttctg
    10921 accgatttcg tcatgcagct ctcgcgccag gttttttcgc gtgtcctcct cggtgtggat
    10981 cagcttttcc gccaatgccc gccgcgctgc cagctcggcc tccagccgtt gtcggtaatg
    11041 gtgcaggttt agcgccagat gttgctggcg actgatggca atgcccaggc caattcccag
    11101 caaggcctgg gtcgcgagga aaatctccag ctccaccagg ttactaaagc ccacgccaat
    11161 ctggcgggcg atggtgatca tcatgctgcc caaaagcccg gagagtacgc caccttgcca
    11221 gccaaatttc cacgccatca ccacattggg caaaaagacc acgatcaaca gcagacgttc
    11281 aatttcaggt gacagcacta tctgagtgcc gataccaata ataaaaaaca ggctacacca
    11341 gatgattaaa gaggtacgca aggggggatt atgcgtatcg agtcccagga gctggtagcg
    11401 atgctgctgg cgcagaaact caaagataag atagacaaac ggcgtcagta atacgccgcc
    11461 ggtaaatgag gtcaaacccg gcagcattat tgggcctgac ataaaagggg agagcagggc
    11521 ggtattcaac aatgaagctg ccgtgacagt ggctaacagc aacgtcagac gctgccagta
    11581 aagtggaaaa cggtgccaga agttttgcgc aaggagggca ggaaacaggc taataaccgg
    11641 cgttgccagc atgagataac cgttcaatag ctgttcacta tggagccaga acatcatcag
    11701 caaagggggg agcaccaacg caggccagta gcggcgagag agcaaaataa caagggcgag
    11761 ataaactccc tgcgggagaa acaacaccgc ctgttgaccg ttattggtca gataaaagct
    11821 gagcgtccac agcatcagcc agccggttcc ccaggccagc acaataaaca gcgaaatcac
    11881 ccaatgacga agattgcgcg acattaatgc cccgccagca gatggtggtc gagcgcaaaa
    11941 tgtaccagtt caatggtgct atggcagttc agttttccca gcacattggc gcgatgcaca
    12001 tgtaccgttt tatgactgag ttcgagttta aaggcgatct ctttaacgct gtctcctttg
    12061 acaagtaaat caaacacttc acgttctcgt ggggtgagaa tttccagcgc ctgggcgggt
    12121 tgcccgccgc cgcgtaaagc ccgaatcgca tcggcacaga gataatgccc gcccagcccg
    12181 accgagcgca ccgcctgtac taactcttcc ggcccacagc gtttggttaa atacccgctg
    12241 gcaccggcat ccagcgcgct ttgcacaaag gttggcgcgt cataaatgct aagaataatg
    12301 gcgcgaaatt gcggtttttg tacacgcagg cgcttaagca aactgaggcc gttttcgtcc
    12361 ggcatggcaa tatcaatgac ggcaacattg acgtcatcac ggattaatgc cgaccatgcc
    12421 tgcgcggcac tactgtactg accaatgacc tccagatcgt cttccagaga gagtaattgt
    12481 gcgaagccag agcgcaccac cacatggtca tccaccagca ctacccgaat catacttttc
    12541 ttctcttatt atctaattct cctatcatgc atcacggcgc ggcgctggca atcaaccagc
    12601 gacagatatg gaatggatgt aacaaaatgg caataatttg ccggagcgcg cacatcttat
    12661 ctggcttgtg aaccggcggg atacgttcgc caaaaccgca aaatgcgcaa actcgctaac
    12721 cattgccgat ttttttctaa aaacgctttg ttcttaacaa gaccttttaa ttcctttatc
    12781 aaactgaccc gcaacgaaat actgctccca taacaagaca ggggagcaga caatcatggc
    12841 aatttcatcg cgtaacacac ttcttgccgc actggcattc atcgcttttc aggcacaggc
    12901 ggtgaacgtc accgtggcgt atcaaacctc agccgaaccg gcgaaagtgg ctcaggccga
    12961 caacaccttt gctaaagaaa gcggagcaac cgtggactgg cgtaagtttg acagcggagc
    13021 cagcatcgtg cgggcgctgg cttcaggcga cgtgcaaatc ggcaacctcg gttccagccc
    13081 attagcggtt gcagccagcc aacaggtgcc gattgaagtc ttcttgctag cgtcaaaact
    13141 gggtaactcc gaagcactgg tggtaaagaa aactatcagc aaaccggaag atctgattgg
    13201 caaacgcatc gccgtaccgt ttatctccac tacccactac agcctgctgg cggcgctgaa
    13261 gcactggggt attaaacctg ggcaagtgga gatagttaac ctgcagccgc ccgcgattat
    13321 cgctgcatgg cagcggggag acattgatgg tgcttatgtc tgggcaccgg cggttaacgc
    13381 cctggaaaaa gacggcaagg tgttgaccga ttctgaacag gtcgggcagt ggggcgcgcc
    13441 aacgctggac gtctgggtgg tgcgcaaaga ttttgccgag aaacatcctg aggtcgtgaa
    13501 agcgttcgct aaaagcgcca tcgatgctca gcaaccgtac attgctaacc cagacgtgtg
    13561 gctgaaacag ccggaaaaca tcagcaaact ggcgcgttta agcggcgtgc ctgaaggtga
    13621 cgttccgggg ctggtgaagg ggaataccta tctgacgccg cagcaacaaa cggcagaact
    13681 gaccggaccg gtgaacaaag cgatcatcga caccgcgcag tttttgaaag agcagggcaa
    13741 ggtcccggct gtagcgaatg attacagcca gtacgttacc tcgcgcttcg tgcaataaaa
    13801 ggaggcgcag atgctgcaaa tctctcatct ttacgccgat tatggcggca aaccggcact
    13861 ggaagatatc aacctgacgc tggaaagcgg cgagctactg gtggtgctgg ggccgtccgg
    13921 ctgcggtaaa accaccctgc tgaatctgat tgccggtttt gtgccttatc agcatggcag
    13981 cattcaactg gcgggtaagc gtattgaggg accgggagca gagcgtggcg tagtttttca
    14041 gaatgaaggg ctactaccgt ggcgcaatgt acaggacaac gtggcgttcg gcctgcaatt
    14101 ggcaggtata gagaaaatgc agcgactgga aatcgcgcac cagatgctga aaaaagtggg
    14161 gctggaaggc gcagaaaaac gctacatctg gcagctttcc ggtggtcaac gtcagcgggt
    14221 gggcattgcc cgcgcgctgg cggcgaatcc ccagctgtta ttactcgacg aaccgtttgg
    14281 tgcgctggac gccttcaccc gcgaccagat gcaaaccctg ctgctgaaac tctggcagga
    14341 gacgggcaag caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac
    14401 tgaactggtt ctgctttcat ccggccctgg acgtgtgctg gagcggctgc cgctcaactt
    14461 tgctcgccgc tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat
    14521 cgccatgcgc gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt
    14581 gtgctcatta atgaaaaact gcattcgcgg cggttgaaat ggcgctggcc gctctcgcgt
    14641 caggtgacct taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg
    14701 gcgctgcaac tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg
    14761 ctcaccattg ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc
    14821 agcctgacgc gcattgtgct ggcgctactc gcggcggtgt tgatcggcat tccggtcggg
    14881 atcgccatgg ggctaagccc gacggtgcgc ggcattctgg atccggtaat cgaactttat
    14941 cgtccggtgc cgccgctggc ttatttacca ctgatggtga tctggtttgg tattggcgaa
    15001 acctcgaaga tcttactgat ctatttagcg atttttgcac cggtggcgat gtcggcgctg
    15061 gcgggggtga aaagcgcgca gcaggttcgc attcgtgccg cccagtcgct gggtgccagc
    15121 cgtgcgcagg tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga
    15181 ttacgcattg gtctgggggt gggctggtct accctggtag cggcggagct gattgccgcg
    15241 acgcgcggtt taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggtg
    15301 ctggcgggga tcgcggtgat tgcgattatc gcctttcttt tagaactggg tttgcgcgcg
    15361 ttacagcgcc gactgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta
    15421 ccccgctggg gccgtatatc ggagcacaaa tttcgggtgc cgacctgacg cgcccgttaa
    15481 gcgataatca gtttgaacag ctttaccatg cggtgctgcg ccatcaggtg gtgtttctgc
    15541 gcgatcaagc tattacgccg ctgcagcaac gcgcgctggc ccagcgtttt ggcgaactgc
    15601 atattcaccc tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc
    15661 ataacgataa tccgccagat aacgacaact ggcataccga tgtgacattt attgaaacgc
    15721 tacccgcagg ggcgattctg gcagcgaaag agttaccttc gaccggcggc gatacgctct
    15781 ggaccagcgg tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagcg
    15841 ggctgcgtgc ggagcatgat tttcgtaaat cgttcccgga atacaaatac cgcaaaaccg
    15901 aggaggaaca tcaacgctgg cgcgaggcgg tcgcgaaaaa tccgccgttg ctacatccgg
    15961 tggtgcgaac gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta
    16021 cgcgaattgt tgatgtgagc gagaaagaga gcgaagcctt gttaagtttt ttgtttgccc
    16081 atatcaccaa accggagttt caggtgcgct ggcgctggca accaaatgat attgcgattt
    16141 gggataaccg cgtgacccag cactatgcca atgccgatta cctgccacag cgacggatta
    16201 tgcatcgggc gacgattctt ggggataaac cgttttatcg ggcggggtaa tacgagagtg
    16261 gacggtcccc tcgccccctt ggggagaggg ttagggtgag ggggcgttca ccgtactttc
    16321 aacaggttaa ctcccccttt ctgagaggaa acaaaattaa cgcagaatct tcttctcagc
    16381 caaatccagc gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa tcgaacctaa
    16441 gctttcgagc accactttct cttcatctat agcacccgcc agcgcggcga acttaatcat
    16501 cgcgtactca ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc
    16561 acgcacgatg tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc
    16621 ttcatccagc agtgattcac gaatcgcctc gcgacggttc attgggttca tctgatagct
    16681 tttgcggtcg ccttttaatg cgcttccggc agcttcacgg aacggaccat aaaaggagga
    16741 ggcgaacttg gtcgaatacg acataatcgc cgtatcttta aagccagcag cgtccagcgc
    16801 ctggcgaatc gcctgtacct ggccatccat cgcggcggaa ggggcgatga agtctgcacc
    16861 tgcagcagct gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac
    16921 gccatgctcg cacagcacac cgcagtgacc gtgagaagtg tattcacaga agcaggtgtc
    16981 tgacatgaca atcatttctg gcactgtctg cttgcagatg cgcgacatac gcgccaccag
    17041 tccatcttcc cgccaggcat cgctgccggt ttcatcggta tggtgagaga tgccaaaagt
    17101 catcacggaa cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc
    17161 tggaatgcgc atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc
    17221 aacaaagatc ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg
    17281 cagcgcagga gatttgcgca ggcgacgagg gcgttggatt aaatctgtca tggtctgcct
    17341 gatgtttgtg gaatcgaggg gcatagtata cctgaagcag ggtagggatg ttttacgaaa
    17401 gttgtcgcga tgttgacaag aagagaatgg aagagagg
//
LOCUS       DAZGBC010000100        17076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGBC010000100 DAZGBC010000000
VERSION     DAZGBC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.101"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(163..699)
                     /gene="yghD"
                     /locus_tag="ACHK1J_003245"
     CDS             complement(163..699)
                     /gene="yghD"
                     /locus_tag="ACHK1J_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO7912480.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIIAARKSATTAPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYVLRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(701..1879)
                     /gene="gspL"
                     /locus_tag="ACHK1J_003246"
     CDS             complement(701..1879)
                     /gene="gspL"
                     /locus_tag="ACHK1J_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7912481.1"
                     /translation="MSSILEIFFPLCASDTIRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGSWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRDQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1876..2853)
                     /gene="gspK"
                     /locus_tag="ACHK1J_003247"
     CDS             complement(1876..2853)
                     /gene="gspK"
                     /locus_tag="ACHK1J_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7912482.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLALQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2850..3455)
                     /gene="gspJ"
                     /locus_tag="ACHK1J_003248"
     CDS             complement(2850..3455)
                     /gene="gspJ"
                     /locus_tag="ACHK1J_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7912483.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVTGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQENWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3452..3823)
                     /gene="gspI"
                     /locus_tag="ACHK1J_003249"
     CDS             complement(3452..3823)
                     /gene="gspI"
                     /locus_tag="ACHK1J_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7912484.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPQNTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3820..4383)
                     /gene="gspH"
                     /locus_tag="ACHK1J_003250"
     CDS             complement(3820..4383)
                     /gene="gspH"
                     /locus_tag="ACHK1J_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7912485.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            complement(4387..4842)
                     /gene="gspG"
                     /locus_tag="ACHK1J_003251"
     CDS             complement(4387..4842)
                     /gene="gspG"
                     /locus_tag="ACHK1J_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7912486.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4859..6082)
                     /gene="gspF"
                     /locus_tag="ACHK1J_003252"
     CDS             complement(4859..6082)
                     /gene="gspF"
                     /locus_tag="ACHK1J_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7912487.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPMHIEA
                     RMNASAGGMLQRRRHAHRRVASADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPVMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6082..7575)
                     /gene="gspE"
                     /locus_tag="ACHK1J_003253"
     CDS             complement(6082..7575)
                     /gene="gspE"
                     /locus_tag="ACHK1J_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7912488.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDAHTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7575..9635)
                     /gene="gspD"
                     /locus_tag="ACHK1J_003254"
     CDS             complement(7575..9635)
                     /gene="gspD"
                     /locus_tag="ACHK1J_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO7912489.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9665..10624)
                     /gene="gspC"
                     /locus_tag="ACHK1J_003255"
     CDS             complement(9665..10624)
                     /gene="gspC"
                     /locus_tag="ACHK1J_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7912490.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTVVSPSVNKPPRTDAKTFDKNDVQLISQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLVEEERSTVAVTNKKAISDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10642..11052)
                     /gene="gspS2"
                     /locus_tag="ACHK1J_003256"
     CDS             complement(10642..11052)
                     /gene="gspS2"
                     /locus_tag="ACHK1J_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO7912491.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQITQTPDAFLTSYQRQMCADPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11118..11927)
                     /gene="pppA"
                     /locus_tag="ACHK1J_003257"
     CDS             complement(11118..11927)
                     /gene="pppA"
                     /locus_tag="ACHK1J_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO7912492.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12254..16816)
                     /gene="sslE"
                     /locus_tag="ACHK1J_003258"
     CDS             complement(12254..16816)
                     /gene="sslE"
                     /locus_tag="ACHK1J_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO7912493.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPIPDPEPTPEPEPEPVPTKTGYLTLGGSQRV
                     TGATCNGESSDGFTFKPGEDVTCVAGNTTIATFNTQSEAARSLRAVEKVSFSLEDAQE
                     LAGSDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKL
                     VNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSE
                     GRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTI
                     ALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNG
                     ATLGEGEQVVNLPNEFIEQFNTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQ
                     GVINKLWGVDEDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKN
                     YWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGN
                     PHYNSILRCPNGYSWNGGVNKDGQCTLSGDSDDMKHFMQNVLRYLSDDKWTPDAKASM
                     TVGTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFE
                     YVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASG
                     FVRLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDT
                     TKEVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKKRI
                     LDAFPGLEECKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAA
                     DLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDEL
                     GFKTFTEFLNCYTNDAYTDGTQCSADLKKSLVDNNMIYGESSGKAGMMNPSYPLNYME
                     KPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGL
                     WAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKF
                     KVPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESASFVY
                     TTPKKNLNASNYTGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRF
                     TNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGAT
                     EVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKE
                     WAEKNFDIKKWYPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDDVGNDKFGNRNYCA
                     ESNGNAADKLMLCASWVAQTDLSEFFKKWNPGANAYQLPGVSEMNFEGGVSQSAYETL
                     AALGLPKPQQGPETINQVTEHKMSAE"
BASE COUNT         3782 a         4843 c         4311 g         4140 t
ORIGIN      
        1 gagtccgggc accactaatt cttaagaacc cgcccacaag gcgggttttt gcttttggat
       61 ctgacaataa ccttcacgaa aaaaattagc ttataaagtc tgggggaatt actctcgcca
      121 cgttaacgag agtaatttta ttgatattaa tctcctgaaa ctttaccctc gtccaaactc
      181 cagccgctgc acattcacca tcccagtctt ctcagcagca ctgacatcaa tttgtgttac
      241 ccgcagcaca tatttttcat ccagtgcgtt taaccatttc agaaggtcat taaacaccac
      301 aggttctatc cagacctgaa tattctcccc acgctcggct atccgcttga tgaccaccga
      361 gtgcgcggaa gcactgtcac tgatgacccg cgatacctgc gcaggtgctg tcgtggcgga
      421 ttttcgcgct gcaataatat ccggcgcggc gctcctcagt cgggtgttca tcgccaccag
      481 ttgctgcaac atcgtctcct gttgttctat ccgttcgctc aacggctgcc agatgaggac
      541 gtaatatccg acgctaaaca ggaacaccac cgctgccagt aacatgccct tttcacgcgg
      601 agaacgcccc gccaggtgtt gtgccagcca gtgttcgcca cggcttaact ggcgttcacg
      661 ccattgctga aaatagtgaa taaatttatc gcgtaacatg ttatttcctc cgcaacgtta
      721 cgccgccgga aaccgcatca ccctctttct gtaacgcgtc ctgttgcaca acataatcgg
      781 ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt tgctgcccgc aactggaggt
      841 gaagcgtctg gcgtttttga tcaaaggtga aaccacgcat ctcgatgtcg gaaagtgacg
      901 ctgatttcag ggtgctggct atcgctgaca actcggcgag cagccgggta tcgtcggcct
      961 gtgggcgata ttttttcagc gccatcgtca cctgagagcg taaattcaca atccgcttct
     1021 gctccgggaa caacgttaag aactgtttct ccgcctggtc gcggctttgc gccacctgtt
     1081 cgctgacgct ccataacgtc acgccccgtt ccactgccag cgcaaccaga atcagcaata
     1141 tcggcagaat catcacccgc cagcgcgccc actgttttcg gtagctgacg cgaggctgcc
     1201 acgaccctgt tagcaggttc ccttccggtt cgccataagt ggtaatggcg ggcagagccg
     1261 taacggtcag gcgttcagca tctgcgccag cccatgctga tagctcttcc ggtgcaatgc
     1321 cgactacggt tagcgaaagc ggtaaatcct gctcatttag ctgggcgcgg aacatgaccg
     1381 gagccagcgc ccgcccggcg ctccatcccc ggcattcatc gatgcggcag ataacccgtt
     1441 gcgcatcgcc agccatatag ccacaaggta tggacatcca gtctggcgcg acggtggcgc
     1501 gggtgatgcc gtttgcctgc aaccactgcg caatgttgcg catatgctgt tggtgaatca
     1561 ctgccacggt tgccagttgc tggtcgattt tcaacggggc gaaatgcagt tcatcgatat
     1621 cctggttcag ctcttcttcc agtaaggcgg gcagaatagt cggtatctgc ttgcgtggga
     1681 catcaggcag ttcaacctgc cagacgctga tccattcgcc gggaatgtag agtcgaatcg
     1741 catcagtttg cagccattgc tggagacgtt cgtcagcaac gtcaggccag ataccgtgtt
     1801 ccacgtcggg tgtacggcgc tgccagcgga tggtgtcgga ggcgcaaagc gggaaaaaaa
     1861 tctcaaggat ggaactcact cactttctcc tgtctgatgc cagagaacag aaaagtgttg
     1921 tgggcccatg cggacaatta acgagttcat cgtcagttca atctcattca cggtgatatc
     1981 tgaacgcagc cagaagtaat tgctgtccac gctcaggacg gtttttagct gttttttagt
     2041 acgctcatcg acgtcagcaa gtagcggctg tgcaagaaac tgatcgacat cttcccagcc
     2101 cttcgccgga cgttgttgta ataacgcccg cgcctgaaca gggcttaacc acgggtcaaa
     2161 cagcgcctca agaatcacac tttgcgtgac gtctaaagta ttgatgttga tttgctggcg
     2221 ggtcatcggc agcgcacaga ccagcggttt cagtttttga taaagcccgg cgtccattcc
     2281 ctgcaccacg cgcatctcgc tgatatcggc cagcggttga ttggcggcgt agaaaggcac
     2341 cgagcgggcg agatattcgc tgtcttcacg gcccagacgc gtctgcacgc tgcggtcttc
     2401 atcaataaac tcccacaggc tttcggctat cagttcggcc cgataagcag gcacatcgag
     2461 gcgcgagatc agggcaatca gttgttgtaa cgcgagcgga cgcgacgccg tcgtcggctg
     2521 agcgagggca ttcaggttaa agcacgcctg tgcgtcacgc agagtgacgg cgatttgccc
     2581 ctgcggcagt gggaaaaaac gagggccgga agcccacggc tgtgccagat gcacgcgctt
     2641 ttcatttttc aggctcagac tgagtgcact cagcgccagg ctttctgcac tggcgctgta
     2701 ccacagcgcc tgctggtact cctgctggct gcgggttcgc ccaagttgtt gctgcatccg
     2761 cccggaaagc gtgatggtta ccagcatcat aaccgccagc aataccagca ccacaaccag
     2821 tgccattccg cgttttggtg gtgaggtgat catgataatt gcggcccgcg taacaaccag
     2881 atgcgttcaa tctcgcccca ttgcggcgaa tgcagggtca tgcgcaccgc cacagggatc
     2941 gcctgtactg acgaccagtt ctcctgccag cgcgtaccat catagaactg caagcggagc
     3001 gaatccgccg gaatcaactt ttgcattgtc ggcttcacgc tgcctgctgc gtcggtcagc
     3061 ggccaggcta accgttcgag atagccgtca tgaatgcggt aaccgacggt gagcagattg
     3121 ctgcgtggca gacgcatcaa cggattaacc acgccgccac gcacaaagcg catcccttca
     3181 ctctcagatg ccagcacgcc agcgccagcc agtaacgctg gttctcgctg gccctgatcg
     3241 cctcttaccg gacgcggcat catttgggtc agatcgtggg tcagaaaact catcgtttgc
     3301 tgcatgaggt ttagtttttg atcgtgtccg gtgacggcgc tattcacgcg cgtgacgccg
     3361 ttagtcacct gctgcgccat cagtgccagc gaggcaaaaa tggcaattgc caccagcatt
     3421 tccagtaacg tgaaaccagc gcgagccctt ctcactgttg gcctcccacg gcgctaaacc
     3481 aggcgcgtcg tgactgaatt accgacgaaa agtcttcgtg aaggctgact tcaatatcca
     3541 ccgcctggag cagcgcgttg cctgtattct gcggtgtggt tcgccagtac cagcggcttc
     3601 cagccatatc gctctcgccc tgatgcctga ccgcccgttg ttcacgggtc atcaggtaga
     3661 gcagcgcggt ctggttttca gccacccagc ctgctaccgt tttctcctca agaacgtgct
     3721 gattactcag cgcaccgctg gcaatctgta acaccgccgt ggcagccagc gcaaaaatcg
     3781 ccagtgcgag catcacttcc agcaaggtaa atccgcgctt catggcatcc tctcatcaca
     3841 ttgactgagg gataacgcgc catcgtgtgc cagttttacc gcccaacatg cgttttgcgc
     3901 cgctgagtag aagcgcagcg taaacggcgt ggcgggttca aaaggcgaaa aacggatctg
     3961 cggcgtcttc tttttcgcct ctttttgcaa ctccagttca atatcgtgca gcgtcaggcg
     4021 acgacgttgc agctccagcg catattcctt ctgccagata tcacttccgg gttgtaacag
     4081 catctgcacc tgtttcggca ccgtaacctg tgccgataac cgggtcgcag aaacgggcaa
     4141 ccactgcccc tgacgacgtt gcataaactg gtatcccggc gggtcgatta acacaccaag
     4201 tgtttgccct tcgatcactg ccctgtcctt aaactgcgca aagcgagtca gaaaatcctg
     4261 cgccgctttt ttcgcgggcg attcagaatc ggtcgcaaaa gtctgtacca cgcccgcact
     4321 ggcaaggccg ataaggaaaa tcaccagcat gatttccaga agtgtgaatc cgcgttcagg
     4381 cactgcttac tgaaactcct gcaaattcca gttaccgata tctgcgccag cgccttcccc
     4441 gttttcctga ccatccgccc ccagggtata aacatcaaac agcccttttt cacccgggct
     4501 gagatactga taatcattgc cccacggatc ctttggcagt cgtttaatgt atccaccagt
     4561 acggtagttg cgggcgtccg ccatattggc tggctgctgg atcagcgcct caagcccctg
     4621 ctcggtagtt ggataacgcc cgttatccag tcggtacata tccagtgcgt tttccagcgc
     4681 cacgatatcg ctgatggctt tttgccgatc cgctttctct ttattgccca acaggttagg
     4741 caccaccaga cttgccagga cgccaagaat aacaatcacc accatcactt ccagcagggt
     4801 aaaacctgcc cgtggttttt gtgtgcggga taacgaattc atgtgataac tccgtaaatt
     4861 acattccaac catattgttc agttgcagca tcggctcgag gatggcgatg acgataaaca
     4921 gcaccacgcc cgccatcatc accaccagcg ccggttcaaa cagccctaac gccagcccca
     4981 cctgggtatc aaattcccgc tcctggttga cagcggcctg ctcaagcatg gtttccagtt
     5041 cgccgctctg ttcgccggag gcgatcatgt acagcatcat cggggggaac aggcgcaact
     5101 ccgccagcgc ggcgcgcaga ctgcttcctt cgcggacgcg atctgccgcc agcagcagtt
     5161 gttgctcgac atagcgattt gccgacacag cggcagcggt ctgaatgcct tccagcagcg
     5221 gaacactgct ggcggtgagg atgcttaatg tgcgggaaaa ccgcgccgta ttcagtccgc
     5281 gcgcaacacg ccctgtcacg ggcaggcgca gcaaggtttt gtcccagcgc aggcgcataa
     5341 caggattttt gagtagccgt tgccccagca ccagaagcgc cagcaatccc gccagccagt
     5401 aaacgccgct ggcctgtaac gcgtcactca tggcgataag cgcgcgggtg gtggcgggca
     5461 gcgcgtgtcc gaggtgatcg aactgttcga tgattttcgg caccactgcc gtcagcaaaa
     5521 tagtgaccac gcccgttgcc accactagca gaaccagcgg atagagcatg gcctgcaata
     5581 gacgtgattt caggcgctgt cgctgttcgg tgtaatccgc caggcgatta agcaccacgt
     5641 cgagatgccc ggatttttct ccggcagcca ccatcgaaca aaacagggaa tcaaagacgc
     5701 ggggatgttc gcgcaggctg tccgacaggg tgtaaccttc ctgaatccgg ctgcgcagcg
     5761 ccattccgag gctttttaca tgcagttttt cactttgctc actgaccgcc tgtaagcagg
     5821 tttccagcgg cattgctgcc tgcaccagcg ttgccagttg gcgcgtgaac agcgccagat
     5881 ctgccgacgc cacgcgacga tgtgcgtgtc gccgacgctg caacattccc cctgccgaag
     5941 cattcatccg ggcttcaatg tgcatgggaa tgagatcttt accgcgcaac aactggcggg
     6001 catgacgcgc ggaatccgcc tcaatcatgc ctttggtttt gcgaccatta cgctccagcg
     6061 cctgatagta aaacagtgcc attacgcctc catggttacc cgcagaactt catcgagaga
     6121 ggtttctccg gcgagcactt tctcaatgcc gttgctgcgg atgcccgcag agtgttgccg
     6181 gacataacgt tccagctcca gttccccggc ctgacggtgg atcaaatcac gcaatgtggc
     6241 atccaccacg atcagctcat ggatggcagt acgtccgcgg aaacctttat gattacaggc
     6301 ggggcagccc tgcggatggt acagagtgac ggtatgcgcg tcggtaattc ccagcaggcg
     6361 tttttcttcg tcagtggcag gcgcgggctg gcggcagtcg gggcacagcg tgcgaaccag
     6421 tcgctgcgcc atcacgcccg tcagactgga agagagcagg aaaggctcca cgcccatatc
     6481 ctgcaaacgc gtgatcgccc ccaccgccgt gttggtatgc agcgtggaaa ggaccaggtg
     6541 tccggtaagt gaagcctgga cggcgatttc tgcggtttcg gtatcgcgga tttcaccgac
     6601 catcaccaca tccgggtcct gacgcaaaat cgcgcgcagg ccacgggcaa aggtcatgcc
     6661 gacgcgggtg ttaacctgtg tctgaccaat tccttcaatc atgtattcga tggggtcttc
     6721 aaccgtgaga atattgcgcg aatggttgtt cagctcctgc aatccggcgt acagcgtggt
     6781 gctttttccg gaacccgtcg gccccgttac cagaaagatg ccgtgcggtt tgtgtaacag
     6841 ctggcgcaac tgcgcggtca gttgctggct aagccccaga cgctccagcg tcaggcgggc
     6901 ctggtttttg tccagcagtc gcagcaccac gcgctcgccc caggcggagg gcatggtgga
     6961 gacacgcacg tcaatcgccc gaccgcccag cagcagcgca atacgtccat cctgcggtac
     7021 gcgcttttcg gcgatatcca gccgtgccat caccttgata cgcgatacca gcagcgaggc
     7081 cagtttgcgc cccgggcgca gcatttcatg taatgtgccg tcaacgcgaa aacggatcac
     7141 cagactcttt tcaaacgtct cgatgtgaat atccgaagcg ccttctttaa tcgcctctgc
     7201 cagcatggcg ttgatcagtt tgatgatcgg cgcatcgtcg tcactttcca gcagatcttc
     7261 cgtttccggc agttcttcag cgagagtaaa aaagtcctca gcagaaccga gatcttccat
     7321 tagctggcgg gcctcggaag agtcccgctg ccagaccgca ttcagtcgct gttcaaattc
     7381 ggcctcgtcg atttgccgca gcgtaaaggg cgcgttcagc ccccgctgca gctcctgcaa
     7441 aacagagagc gacaacggat gcacatggag gatctccagc gacgcttcgc accatgccac
     7501 caggctaaac cgacggctga aactgtaggg cagacgcacg gtgttagcgg tggtttcctg
     7561 tgctacaggc accattaacg cgttctcccg gcattaagga acgcacgtac ttccggcggt
     7621 aaggcctggt tttgcgctgg cagtaccggc tgcgcggtgt gcggcatcag gcttaagcct
     7681 tgctcatcgc gatagatctg ttcggcgcgc atatagttat atttgcgctg cgacacgccg
     7741 tctgccgcca taccgtcacg cagaatggtc ggacggataa acaccatcag gttacgtttt
     7801 tcttttttat ccgccgtcga tttaaacagg ttaccaatca acgggatatc gcccagcagc
     7861 ggcactttcg ccacactttc tcccgcctgg tcgtccatca gaccgccaag cacgatcagt
     7921 tcaccatcgt tagccagcac ggtggtcttc agtttgcgct caccaaacac cacgtcgagg
     7981 ctggtctgtc cttccacctt cgagacttcc tgctcaatca ccatctgtac cgcgttacct
     8041 tcgttaatct gcggcgtgac tttcagcatg atgccgactt ttttcctctc tactgtattg
     8101 aaaggattgc tgttattgga gccaacggta gagccggtta ataccggaac gtcctgaccc
     8161 accatgaaga aggcttcctg gttgtccagc gtggtgatgc tcggcgtgga gagaacgttc
     8221 gagctggagt cgtttttaac cgcctgtacc agcgccatcc agtcgccttt caccacgcca
     8281 accgccgtac cgctaaagcc ggaaagaagc tgggcgagcg tggagagatc gccgttggta
     8341 tccggattaa tggtggttgc gccgttttca ctgatcaccg tcgagccttt ctgcggtttc
     8401 gcctgagaaa tggctgcgcc cagcgtaccg atagggatct gcgtaccatt agcaaactgc
     8461 attaatccgg catctttcga cgcccactgc acgccgaagt tgatattact gccttcggcc
     8521 acttccacga tcaacgcctc gacgtgcacc tgagcacggc gaatatccag ttgttcaatc
     8581 acgctttgca gcgactgcat gatgtcctgc ggcgcggtta caatcagggc attactgtgt
     8641 ttgctggcgg cgatggagac aacctcacgc ccgctaccga ccgtgccttc cgcctcttct
     8701 ttagccgccg tgagcgtgcc gctgacctgc ttcagtacat cgaccagatc ttcggctttg
     8761 ctgtatttga gatagaaaac ctggctgttg ccgctgcgct ccatttctga gtccagccga
     8821 cggatcaggc ggcgcatttt gtcccgcgtg gccgggtcac cactgacaat cacactgttg
     8881 gtgcgttcgt cggcgacaat ttgagatttc agcgtcgcag gctggttctc gccgctgttt
     8941 ttcgtcaggc tttccagcac gcgggcgatt tccgaagccg aggcgttatc cagcgggatc
     9001 acctcttcag tgcgattacc cgcgtgatcc acacgctgga tcacttccgt cagccgctcc
     9061 acgacggagg cgcgtccggt gagcataatc acgttggagg gatcgtaatt aacaacgttg
     9121 cctgagcctg cgctgtcgat catctggcgc agaatcggtg ccagctcgcg taccgacaca
     9181 ttacgtaccg gcactacttt ggtgaccatt tcatcgcccg cgtagttgtc gctgccttca
     9241 ccaaccagcg gcagcggctc gactttcgcg gcactggatt tcaccacctt cagcacgtcg
     9301 ttttccatcg gtacgaccgc atacccctgt gcttccagca ggttaaggaa taactggtaa
     9361 tactggcgtt cattgagcgg agtcatagtg cgaatactca ctttcccctg aacgcccggc
     9421 cccataatga tggttttatt aaggttagcg ccgacggttt cgatgaacga tttcaggtcg
     9481 gtatctttaa aattagcggt gaaagtggct tcttccgccc agaccggtga actgcataat
     9541 gccgctgcca acaccagcgg cagtaaacgc ttttttgttt tgaggccagt tgtcttctta
     9601 cgccagaccg acaacgtaat atcacgccaa aacacgatga atgattctcc tggattaaat
     9661 gcggttagcg cagcgcgatg gaaatgtcgt ggcgcgcacc cttgcgtaaa accgtaagtt
     9721 gaatggaatc cattgaaggt aactgccgca tcagagcaat cattgctcgt ggatcagtga
     9781 aatcctgctg attgagcgca atggcgatat cgccttcctt gaaaccgctg gcatcgaaca
     9841 gagaacgatc tgcccccggt ttcactgcat aaccgacaat tccctcctta cgcacaggcg
     9901 taagctggat atagttaaaa attttctgcg gatctttcgc cagtgcctga cgcacggcag
     9961 tcgggatctc aactggcgca ctgacagcag gttcagcaac agcttgcttt gcttcgtcac
    10021 tgatggcttt tttgttggtc acggctacgg tggaacgctc ctcttcaacc aggctcaggc
    10081 gctctatttt tccctgatag cgcagcatca catggtcgcg gttgatttcc tcaatcaccg
    10141 cgttgtgaga accaagccgt tccccctgca aatagacctg ctgtttgcca ccttcttcaa
    10201 taaccgcgcc gggtctggca ccaaaggcga tcccgcgcag caccacatta agacgcgttt
    10261 cggccacagg tgcaggttcg ggttgtttta ccggtgcggc gacgggctga tatttgccaa
    10321 accagttttg ctggctgatt aattgcacgt catttttatc gaacgttttt gcatccgtac
    10381 ggggcggttt attcaccgat ggagaaacca ccgtatattc cgcagaaaag gagaaataac
    10441 gccagagtga atgcgccact tttgcagaaa taataagcag catcagccag aacatcccgc
    10501 gtgcaatttt tcgcagatgc tctttgtctg tattaagaga ttgtctctgg ttaagagtgt
    10561 gacgaatttt tgtcagccat tgaatgagat aaattcgtgc gtcacgaaaa acaacccgcg
    10621 ccaatttata tctacccgac gttatgcttt gactattcca caggtggtac gatccagttt
    10681 ccgctgatac tggttacctg tacgtgtatc attaatcgtt atgctgtaat taattccctc
    10741 agtgagcatt aatttcaccg ttgggtcagc gcacatttgt cgttgatagc tggttaaaaa
    10801 ggcgtctggc gtctgcgtga tctgagtacc cgcttcgctg ataatggtca tttttaccgt
    10861 cgtgccgcta ctttgtgcca gcactaaggt atatcccgca gatttaatcg gcaggttttg
    10921 gctgatattt tgcgcctgtt ttttcgccag taaactggca ttttcattat ggccggcaca
    10981 gccacttaat aatcctgtaa cgctcaacaa tagcgatatt aataccctcc ctggcatttg
    11041 ttttatcgac atggtaaata atctctaagg ttattaataa gagttaaaat gtcactttga
    11101 taatgacgtg gttatcatta aaacaatgcc tgtagataaa gtgttgctat accgcccaga
    11161 cttaaacacg gtccaaaagg cagtgtggtt gatcctcttt ttgtaataac ggcatatatc
    11221 aggccgcagc atgaggcgat taaagcaaca ttgggcagtg ataacggtcc cacccaacta
    11281 cctaaagcgg cgaacaataa tacatcgccc atgcctaatg cttctttacg cagaactatt
    11341 ccggctatcc agcgcagaga gtaaaaagtg ataaacccca ccaggacgcc ggtgactgca
    11401 tcttgtagcg tgagcgggct ctgctgcgcc catgccgcaa ttagtcccgt ccacaatacg
    11461 ccctgagtaa aaacatcggg cagccattgg tgatcgaggt cgatgatgct cgcggcaatc
    11521 agccaggcgg ataatatcat caccgccagc gcccatccac tttccggcca caccaggctc
    11581 gccagcaaaa aagcgagtgc cgtcaataac tccaccagcg gataacgctt gctgattttc
    11641 gcctgacaat cacggcagcg ccctttgagc atcagccagg agagcagcgg aatattgtca
    11701 cgtatccgga tggtctgctg acaatgtggg cagtgcgaac gcggtagcgc aaggcttatt
    11761 tttgactgcg cactcggcat ttcaccatga aactccgcca tttgttggcg cagcatgatg
    11821 gggtaacgcc aaatcaccac attcaaaaaa ctgccgatga tcaatcctcc gacggtagcc
    11881 agtatgggca tcgccacggg gtattgctga aaaacatcaa aaagcatggt taaaggttgt
    11941 ttgttgtaac ttgccggatg cggcgtaaaa cgccttatcc ggcctacagg tatctgccaa
    12001 cgcaagtaat cgcggctttc cctgaagacc cgataagatg gcgcatcggg catatgtgtt
    12061 aacccgatgc gccttatttc atgccggatg cggcgcgagc gccttatccg gcctacaggt
    12121 gtctgccaac gcaaataaac gcagctttcc ctctctgtag gcccgataag cttgcgcatc
    12181 gggcatatgt gttgacccgg tgcgccttat ttcatgccgg atgcggcgcg agcgccttat
    12241 ccggcataca ggcttactcg gctgacatct tatgctcggt aacctgatta atggtttccg
    12301 gcccttgctg cggtttcggc agaccaagcg ccgccagcgt ctcgtaagcc gactggctca
    12361 caccgccctc gaagttcatc tcgctcaccc ccggcagctg gtaagcattc gcgcccggat
    12421 tccatttttt aaagaactcc gaaagatccg tctgggcgac ccaggaggca cacagcatca
    12481 gcttgtcggc agcgttaccg ttggattcgg cacagtagtt tctgttgcca aatttgtcat
    12541 tgccaacatc atcgccgcgt gctttacgat gcatcaactg gaacaggttc cagcctttca
    12601 tcccttcacg ctcgctgtaa aactctggca gaggagtgcc atctggatac catttcttga
    12661 tatcaaagtt tttctctgcc cattccttca gctgcgcgta catcagcaaa cggtcacccg
    12721 caccgccgcg cgcccaagcc tggttgttgc tctcctccag atattccggc gcgacggtaa
    12781 tatcgtcagc gacacggttc atcttgccga gatagcgatc ctgcatgtac agcgccagca
    12841 cgttgttcgc cacttcggtc gcgcctggaa cagtcagcgg cgtttctgcg gcgttgtgcc
    12901 ctacttcgtg ccagatcaac cagtcgttca gcggcgtcgt cggcagcgtg gtgctgttcg
    12961 tcgagaagct gctgttcatt accggataac ccgagtgcgc atcaccgatg gagatctgca
    13021 catcgtttgt gaaacgatgt ttatgtcccg tcaatgcttt ataggtaaac atccggtgct
    13081 taccgctcgt ttcatcacga ccgtagaagt cattcatcga gctggcaaag gtgtccagat
    13141 cgttagcgaa ttgctccagt ccgccagtgt aattgctggc attcaggttc ttcttcggtg
    13201 tggtatagac gaacgaggca gactccagtt cgcccagcgg agccggtgag ttcagatcgt
    13261 ttttccatgc gccgtcttta tagaacggtg cttttaccac gccagtaaag gtgaagctgg
    13321 cagattcatt ggtagagcta ttgcccttga tataaatcag gccaccgtaa ggcaccttga
    13381 acttcaccgt accgctagcg tccagagagt acgttttagt cactcttggc ggacggttca
    13441 gcgcaacttc atgcttctca cgtccggtca ggtcgtcagc cagcgccacg gtgacggtca
    13501 caggaacgtt cgcattggac ttaatggtga cctctttctg agccggtgcc cacaggccgg
    13561 ttgattgcat gttacccgca aaccatttgg tcggattcga gtacaggctg atggtttcag
    13621 taacgttctg tccctcttcc gatactgctc ccggatactt ctccacatca accttaatgt
    13681 tcagatccca ccaggaacgg cccagcatca ggcgcgtcag cggtttttcc atatagttga
    13741 gtggatagct tgggttcatc atgcccgctt taccgctact ttcaccgtag atcatgttgt
    13801 tatcgaccag cgattttttc agatctgcgg agcactgtgt gccatcggta taggcatcat
    13861 tggtatagca gttcaggaac tcggtgaacg ttttaaagcc cagctcgtca tttttgcctt
    13921 cttcataacg atagctcgta tcgttccaca gccagaccga catgttttgg tacagacgtt
    13981 ccagatcgac gctgctcaga cgctcacctt tgcgaccatt ggtccggaag tagagctcat
    14041 gctgatacag acgctgaatg ttggtgccta aatctgcagc ctgcaccatc gcttttgcag
    14101 tgtcggcgtt aaggcttagt tgcgtatact gtggaacata catgccacca gtaaccggaa
    14161 ctcccgtgcc aggacgatat tccaggcagt tgacctcgta gtggtagtca gaatccttac
    14221 actcttccag ccctgggaac gcgtccagaa ttctcttctt cgccgcagcc agtgactcag
    14281 gcgttttgtg gtcggcttca tcgataaagg cgaattgagt tacctgttta ccctcaactt
    14341 cttcctgcca gctggcaact tccagcttcg gtttgtcatc aggtttgttt tcttgctgat
    14401 atttccagat aacttctttc gtggtgtcat caatggtata cggtggttta ccatcgacag
    14461 ccggataacg ctcatagacc caaattggcg ttgaacgtcg ttgacgaacg cggtccgggt
    14521 agccttgcgg atcgttattt actaccgact tgttaagcgc catcgacaaa cctgcggcat
    14581 caagcagacg tacaaagcca gatgcgctct cttccttaag attgctcatc acgttttcca
    14641 tgatcagcac cgatccacct ttgttcatat aggcgatcaa atcggtcaca tcctgctgag
    14701 tcagcttcgg tttgctggta tctgcacgca gcgggattgc ataaggatca ttaccaacct
    14761 gagtcacata ctcaaagccg ttgaggatca gcagcggcat ttcctgcggg tcgagatcgc
    14821 catagctact taaatgctca acagagatac ccgcaaaatc cggatgaaag ccgaactcag
    14881 cgctgtttcc tgtaacctga ccatgacgtt tgaaatagac agtatccagg ttggtgccta
    14941 cggtcatgct ggctttcgcg tccggcgtcc atttatcgtc ggacagatag cgcagtacgt
    15001 tctgcatgaa gtgcttcatg tcatctgaat caccgctgag cgtacactgc ccgtctttat
    15061 taacgccccc gttccagctg taaccgttcg ggcaacgcaa aatgctgttg tagtgcgggt
    15121 taccgataac catcagtttg ccctcaccga cttgccccag cgaaataaac ggcaggttaa
    15181 aggtggcggt atcgcgcgta acgttttccg gctcaacaag agaaggcgct tccgtaatgt
    15241 acgccagctc gtttttatcc caggcgcgtt tttcgccaaa cgccagccag tagtttttat
    15301 cattacgcgc catcagaatt gggaaggccg cgttggagat attcaccacc gcctgaccgc
    15361 gcgcattacc agtgctgcca tagaagttgg tagagtcatg aaagacgtgg aatttcgtca
    15421 ccgatttgta atcttcatcc acaccccaca gcttgttaat aacgccctga atctggccgt
    15481 cattaacatt gcgcgtcgtc agcgagaacc agcgagcctc gttacaacca tcggttttcg
    15541 cacaaatcgc ggtatcgatc tctttggcct gacccgtatt aaactgctca ataaattcgt
    15601 ttggcagatt aacaacttgc tcaccttccc ccagcgtcgc accgttggat aacgagagat
    15661 tgataatctc gttgatcacg ttgggatatt cggcaaagac cttgcgtaca tcgtccggaa
    15721 caacacgggt attattttgc ccggtcgtcg aatagcgatg aataagctga tcaatattcg
    15781 ccccgcgaac ttcatcaccc agttcagtca gcgcaatggt cgacttattg ccgcgcactg
    15841 aacccagttc aaaggtatcg ataccaaagg agatggtttc gccccagcta aaggaaaatt
    15901 caccattttc ccctgtcacg ccacggcctg aattggtgta gtagttgacg ccagcaacac
    15961 catatccctg gctatcgacc aggcgacctt ctgagagaat gatttccgag ggttgatact
    16021 gataaaactg ttccgcgtta gccgacacga aggaagcgtt cagatccggt tttgttcccg
    16081 gcgtggtgac gggcacgacc ggtgaagtat gagtggatgg cgctttatcg gtcgcagcat
    16141 tgttttccac ctcttcattg accagctttt tgaactcttc cggtgccaga tcgatttgct
    16201 tatacagcga gtcgaagcgt ttactctcga tcaccgagga gaacgtcaga caaacctgtt
    16261 ctgtattcgc cggacagctg ttactggacg ttaccagcga aaccgcattg cttttcttgt
    16321 catcggagcc cgccagttct tgcgcgtcct caaggctaaa cgacactttt tcaaccgcac
    16381 gcaagctacg cgcagcttct gactgagtgt tgaaggtggc aattgtcgtg ttacccgcca
    16441 cgcaagtaac gtcctcgcca ggtttaaatg taaagccatc gctggattca ccattacagg
    16501 tagcaccagt tacccgctgg cttccgccca gggtcagata acccgttttc gtaggaacag
    16561 gttctggctc cggttctggt gttggttcag gatcaggtat cggttctggc gtaggctctg
    16621 ggtccggcgt tggctcaggc gtcggctccg ggtttggtgt tggatcaggt ttcacttccg
    16681 gcaaagaccc tgttccagaa tctacaggcg gcgtatcgga ggaagatccg gagccaccgc
    16741 catcacaacc ggctaacagg gttgcactca aaatagccgc taaaagcgat ttcttatatt
    16801 taaatttctt attcattaat aacgcaagtg acaaaacaat gttaaaaaat tcgtaactgg
    16861 gagaaatagt tttatgcttt atcttcttta ataatttcct ccattcttaa gaaaacgaca
    16921 tcattgatag aaaacaggtg aaatttataa gaataacccc tatacgatgt ctatctggct
    16981 atttttacga aatttcaata aacaaaaaat taacaacatt tcacaacgta actatataaa
    17041 ttcgttggta gttttaacga ttaagattgc aaaagc
//
LOCUS       DAZGBC010000101        17029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGBC010000101 DAZGBC010000000
VERSION     DAZGBC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.102"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..1184
                     /gene="arnB"
                     /locus_tag="ACHK1J_003259"
     CDS             45..1184
                     /gene="arnB"
                     /locus_tag="ACHK1J_003259"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO7912494.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1188..2156
                     /gene="arnC"
                     /locus_tag="ACHK1J_003260"
     CDS             1188..2156
                     /gene="arnC"
                     /locus_tag="ACHK1J_003260"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO7912495.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2156..4138
                     /gene="arnA"
                     /locus_tag="ACHK1J_003261"
     CDS             2156..4138
                     /gene="arnA"
                     /locus_tag="ACHK1J_003261"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO7912496.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPVCTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4135..5025
                     /gene="arnD"
                     /locus_tag="ACHK1J_003262"
     CDS             4135..5025
                     /gene="arnD"
                     /locus_tag="ACHK1J_003262"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO7912497.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5025..6677
                     /gene="arnT"
                     /locus_tag="ACHK1J_003263"
     CDS             5025..6677
                     /gene="arnT"
                     /locus_tag="ACHK1J_003263"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO7912498.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6674..7009
                     /gene="arnE"
                     /locus_tag="ACHK1J_003264"
     CDS             6674..7009
                     /gene="arnE"
                     /locus_tag="ACHK1J_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO7912499.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7009..7395
                     /gene="arnF"
                     /locus_tag="ACHK1J_003265"
     CDS             7009..7395
                     /gene="arnF"
                     /locus_tag="ACHK1J_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO7912500.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7389..7655)
                     /gene="pmrD"
                     /locus_tag="ACHK1J_003266"
     CDS             complement(7389..7655)
                     /gene="pmrD"
                     /locus_tag="ACHK1J_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO7912501.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7765..9120)
                     /gene="menE"
                     /locus_tag="ACHK1J_003267"
     CDS             complement(7765..9120)
                     /gene="menE"
                     /locus_tag="ACHK1J_003267"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO7912502.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9117..10079)
                     /gene="menC"
                     /locus_tag="ACHK1J_003268"
     CDS             complement(9117..10079)
                     /gene="menC"
                     /locus_tag="ACHK1J_003268"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO7912503.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRG
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10079..10936)
                     /gene="menB"
                     /locus_tag="ACHK1J_003269"
     CDS             complement(10079..10936)
                     /gene="menB"
                     /locus_tag="ACHK1J_003269"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO7912504.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10951..11709)
                     /gene="menH"
                     /locus_tag="ACHK1J_003270"
     CDS             complement(10951..11709)
                     /gene="menH"
                     /locus_tag="ACHK1J_003270"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO7912505.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQRLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11706..13376)
                     /gene="menD"
                     /locus_tag="ACHK1J_003271"
     CDS             complement(11706..13376)
                     /gene="menD"
                     /locus_tag="ACHK1J_003271"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO7912506.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRVCDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13465..14760)
                     /gene="menF"
                     /locus_tag="ACHK1J_003272"
     CDS             complement(13465..14760)
                     /gene="menF"
                     /locus_tag="ACHK1J_003272"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO7912507.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVFARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14839..15144)
                     /gene="elaB"
                     /locus_tag="ACHK1J_003273"
     CDS             complement(14839..15144)
                     /gene="elaB"
                     /locus_tag="ACHK1J_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO7912508.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15199..15660)
                     /locus_tag="ACHK1J_003274"
     CDS             complement(15199..15660)
                     /locus_tag="ACHK1J_003274"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7912509.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15725..16642
                     /gene="rbn"
                     /locus_tag="ACHK1J_003275"
     CDS             15725..16642
                     /gene="rbn"
                     /locus_tag="ACHK1J_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO7912510.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16830..>17029
                     /locus_tag="ACHK1J_003276"
     CDS             16830..>17029
                     /locus_tag="ACHK1J_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO7912511.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAED"
BASE COUNT         3940 a         4612 c         4366 g         4111 t
ORIGIN      
        1 agcaaatata agtggacagg tattcaatgg cggaaggaaa agcaatgtca gaatttttgc
       61 ctttttcgcg accagcaatg ggcgtggagg aactcgctgc agttaaagag gttctcgaat
      121 ccggttggat cacaaccggt ccgaagaatc aggcgcttga gcaagccttt tgccagttga
      181 cgggaaatca gcatgccatc gcggtcagtt cagccaccgc cggaatgcat atcacgctaa
      241 tggcgttgga aattggcaag ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa
      301 ccctcaatat gatttccttg ttgggtgcaa cgccggtaat ggtggatgtc gatcgcgata
      361 cgctgatggt cacgcctgaa gcgatcgaat cagccattac gccacgcact aaagccatca
      421 ttccggtgca ttatgccggt gcgccagcag atattaacgc cattcgcgcc attggcgaac
      481 gttacggcat cgcagttatc gaagatgcag cccatgccgt cggtacgtat tacaaagggc
      541 gacatattgg cgcaaaaggt accgctattt tttcgtttca cgccatcaaa aatattacct
      601 gtgcggaagg tggcctgatt gttactgata atgaaaatct tgcccgccag ctacggatgc
      661 tgaaatttca cggtctgggt gtcgatgcct atgacagaca aacctggggc cgtgcaccgc
      721 aggctgaagt cttaacaccg ggctataagt acaatctgac cgatattaac gccgcgattg
      781 ccctgacaca gttagctaaa ttagagcacc tcaacacccg tcggcgcgaa attgcccagc
      841 aatatcagca agcactggca gctctcccct ttcagccatt aagccttccc gcctggtcgc
      901 acgttcacgc ctggcatctg tttattattc gtgtcgatga acaacgttgt ggtatcagtc
      961 gaaatgcgtt gatggaagcg ttaaaagaaa gaggtattgg taccgggtta catttccgcg
     1021 ccgctcacac acaaaaatat tatcgcgagc gttttcccac gctgtcgtta ccgaataccg
     1081 aatggaatag cgaacgcatc tgttcgttgc cgctgttccc ggatatgact accgccgatg
     1141 ccgaccgtgt catcacagcc cttcagcaac tcgcaggaca ataagccatg tttgaaatcc
     1201 accctgttaa gaaagtctcg gtggttattc ccgtttataa cgagcaggaa agcttaccgg
     1261 aattaatcag gcgcaccacc acagcctgtg aatcgttggg gaaagagtat gagatcctgc
     1321 tgattgatga cggcagtagc gataattccg cgcatatact ggtcgaagcc tcacaagcgg
     1381 agaacagcca tattgtgtct attttgctta accgcaatta cgggcaacat tcagcgatta
     1441 tggcgggatt cagtcacgtt actggcgact taattattac ccttgatgcc gatctccaga
     1501 atccgccaga agaaatcccc cgcctggtgg caaaagccga tgaaggttac gacgtggtag
     1561 ggactgtacg ccagaaccgc caggacagct ggtttcgcaa aaccgcttcg aagatgatta
     1621 accggcttat tcagcgcacc accggcaaag cgatgggtga ctacggttgt atgttgcgcg
     1681 cctatcgccg tcatattgtc gatgcgatgt tgcactgcca tgaacgcagc acctttatcc
     1741 cgattctggc gaatatcttc gcccgccgtg ccattgaaat tccagtgcat catgccgagc
     1801 gtgagtatgg tgaatccaaa tacagcttta tgcgcctgat taatttgatg tacgacctgg
     1861 tgacctgcct taccacaacg ccgctacgta tgctgagtct gctcggcagc attattgcga
     1921 ttggaggttt tagcattgcg gtgctgctgg tgattttacg cctgaccttc ggaccacaat
     1981 gggcggcaga aggtgtcttt atgctatttg ccgtgctgtt tacttttatt ggcgctcagt
     2041 ttatcggcat gggattgctc ggtgaatata tcggcaggat ctacaccgat gtccgcgccc
     2101 gcccccgcta ttttgttcag caagttatcc gtccatccag caaggaaaat gaataatgaa
     2161 aaccgtcgtt tttgcctacc acgatatggg atgcctcggt attgaagccc tgctggctgc
     2221 cggttacgaa attagcgcca tttttaccca taccgataat cccggtgaaa aagcctttta
     2281 tggttcggtg gctcgtctgg cggcggaaag aggcattccg gtttatgcgc cggataacgt
     2341 taatcatccg ctgtgggtgg aacgcattgc ccaactctca ccagaggtga ttttctcttt
     2401 ttattatcgc catcttattt gcgacgagat tttgcagctc gctcccgcag gtgcatttaa
     2461 tctgcacggt tcgctgttac caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt
     2521 gaacggtgaa acagaaactg gcgttacatt gcaccgaatg gtaaaacgtg ccgatgccgg
     2581 ggccattgtg gcgcaactgc gcgttgccat tgcgccagac gatatcgcta ttacgctgca
     2641 tcataaattg tgccatgccg cgcgccagct actggaacag acattacccg ccattaaaca
     2701 cggtaatatt ctggaaatcg cccagcgcga aaacgaagcc acctgttttg gtcgcagaac
     2761 gccggatgac agtttcctcg aatggcataa gccggcatcc gtactgcaca acatggtacg
     2821 tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt
     2881 ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag ccggggagcg tgatttctgt
     2941 tgcgccactg ctgattgcct gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg
     3001 cgacggcatt actatgcagg gctcgcaatt agcgcagacg ctgggcctgg tgcaaggttc
     3061 acgcttgaat agccagcctg tctgcaccgc ccgacgccgt acccgggtac tcatcctcgg
     3121 ggtgaatggc tttattggca accatctgac agaacgcctg ctgcgcgaag atcattatga
     3181 agtttacggt ctggatattg gcagcgatgc gataagccgt tttctgaatc atccgcattt
     3241 tcactttgtc gaaggcgata tcagtattca ttccgaatgg attgagtatc atgtcaaaaa
     3301 atgtgatgtc gtcttgccgc tggtggcgat agccacgccg attgaatata cccgcaaccc
     3361 gctgcgcgta tttgaactcg attttgaaga gaatctgcgc attatccgct actgcgtgaa
     3421 gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa
     3481 atacttcgat gaggaccatt ctaatttaat cgtcggcccg gtgaataaac cacgctggat
     3541 ttattcggta tcaaaacaat tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg
     3601 tttacagttc accctcttcc gcccgtttaa ctggatggga ccgcggctgg ataaccttaa
     3661 tgcagcgcga attggcagct cccgcgcgat tacgcaactc attctcaatc tggtagaagg
     3721 ttcaccgatt aagctaattg atggcggaaa acaaaaacgc tgctttactg atattcgcga
     3781 tggtatcgag gcgttatacc gcattatcga aaatgcggga aatcgctgcg acggcgaaat
     3841 tatcaacatt ggcaatcctg agaacgaagc gagcattgag gaactgggcg agatgctgct
     3901 ggcgagcttc gaaaaacatc cgctgcgcca tcatttccca ccgtttgcgg gctttcgcgt
     3961 tgtcgaaagt agcagctact acggcaaagg atatcaggac gtagagcatc gtaaaccgag
     4021 catccgcaat gcccaccgct gcctggactg ggagccgaaa attgatatgc aggaaaccat
     4081 cgacgaaacg ctggatttct tcctgcgcac cgttgatctt acggataaac catcatgacc
     4141 aaagtaggct tacgcattga tgtcgatacc tttcgtggca cccgtgaagg cgtgccgcgt
     4201 ctgctggaaa tcttgagtaa gcataatatt caggccagca tttttttcag cgtcggcccg
     4261 gacaatatgg gccgtcatct ctggcgactg gtgaagccac agtttttgtg gaagatgctg
     4321 cgctcaaacg cggcatcgct ttatggctgg gatattttac tggcaggtac ggcctggcca
     4381 ggtaaagaga ttggtcatgc caatgccgat atcattcgtg aagcggctaa acatcacgaa
     4441 gtcggcctgc acgcctggga tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg
     4501 caaacaatga tcgacgatat tgcacgcggc cttcgcactc tggaagagat tatcggtcaa
     4561 ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc agcaggtgat cgaagcaaaa
     4621 gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg ccatgccgtt ccgtccactg
     4681 ctcgaatcag gaaaccctgg cactgcgcaa attccggtga ccttacccac ctgggatgaa
     4741 gtgattggtc gggatgtgaa agcagaagat tttaacggtt ggttactcaa ccgcatcctg
     4801 cgagataaag gcacgccggt ttataccatt catgcagaag ttgaaggctg cgcttatcag
     4861 cataattttg tggatctcct caaacgcgca gctcaggaag gcgtgacatt ttgcccttta
     4921 agcgaactgt tatcagagac gttgccgctc ggacaagttg ttcgcggaaa tattgccgga
     4981 cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt
     5041 accttatcgg cctcttcgcg tttattgcct gctattacct gttaccgatc agcacgcgtc
     5101 tgctatggca acccgatgaa acgcgttatg cggaaatcag tcgagaaatg ctggcatccg
     5161 gcgactggat tgtgccccat ctgttagggc tacgttattt cgaaaaaccc attgccggat
     5221 actggattaa cagcattggg caatggctat ttggcgcgaa taactttggt gtgcgggcag
     5281 gcgttatctt tgcgaccctg ttaactgccg cgctggtgac ctggtttact ctgcgcttat
     5341 ggcgcgataa acgtctggct ctactcgcca cagtaattta tctctcattg tttattgtct
     5401 atgccatcgg cacttatgcc gtgctcgatc cgtttattgc cttctggctg gtggcgggaa
     5461 tgtgcagctt ctggctggca atgcaggcac agacgtggaa aggcaaaagc gcaggatttt
     5521 tactgctggg aatcacctgc ggcatggggg tgatgaccaa aggttttctc gcccttgccg
     5581 tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa acgctggaaa gatctcttta
     5641 tttacggctg gctggcggtt atcagttgcg tactgacggt tctcccctgg ggactggcga
     5701 tagcgcagcg ggagcctgac ttctggcact attttttctg ggttgagcat attcaacgct
     5761 ttgcactgga tgatgcccaa catagagctc cgttctggta ctacgtgccg gtcatcattg
     5821 ccggtagcct gccgtggctg ggattactcc ccggtgcact atacacaggc tggaaaaacc
     5881 gcaagcattc cgcaaccgtc tatttgttga gctggacgat aatgccgctg ctgtttttct
     5941 ccgttgctaa gggtaaattg cccacctata ttctttcttg ctttgcacct ttggcaatgc
     6001 tgatggcgca ttacgctttg ctggcagcaa aaaataatcc tctggcgctg cggattaatg
     6061 gctggattaa catcgctttt ggcgtcactg gcattattgc cacatttgtg gtctccccct
     6121 ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct
     6181 ggtcgatttt ttcgctatgg gcatttttcg gctggtacac cttaacaaac gtcgaaaaga
     6241 cttggtcttt tgccgcgctt tgcccgctgg ggctggcgtt gctggtagga ttttcaattc
     6301 ctgacagagt catggaagga aaacatccgc aattttttgt cgagatgaca caagaatcac
     6361 ttcagccaag ccgctatatt cttactgata gcgtcggtgt tgccgcaggt ctggcatgga
     6421 gcctgcaacg cgatgacatc atcatgtatc gccagacagg tgagttgaaa tacggcctta
     6481 attatccgga tgcgaaaggg agatttgtca gcggtgatga gttcgcaaac tggcttaatc
     6541 aacatcgtca ggaggggatt attacactcg tgctttcggt tgaccgcgat gaagatatca
     6601 acagtctcgc cattccgccc gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc
     6661 agtatcgtcc caaatgatct ggctaacatt agtctttgcc agcttgctta gcgttgccgg
     6721 gcagttgtgt cagaaacagg caacctgctt tgtggcgata aacaaacggc gcaaacatat
     6781 cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt gccatggtgc tctggctgct
     6841 ggtcttgcag aacgtaccgg taggcattgc ctacccgatg ttaagtctga attttgtctg
     6901 ggtgacgctg gctgcagtaa aactgtggca cgaaccggta tcgccgcgtc actggtgtgg
     6961 ggtggcgttc attattggtg gcattgtgat cctcgggagt acggtgtaat gggcctgatg
     7021 tggggattat tcagcgtcat tattgcctca gttgcgcaat taagcctggg ttttgcggcg
     7081 agtcatctgc cgccgatgac gcacttatgg gattttatcg cggctctgtt agcttttggg
     7141 ctggatgccc ggatactgtt actcgggctg ctgggttatc tgctttccgt tttttgttgg
     7201 tataaaacgc tacataaact tgcgctaagc aaagcctatg ccttgctcag tatgagttat
     7261 gtgctggtat ggattgcttc aatggtactt cccggctggg aagggacttt ttcgctgaaa
     7321 gcactactgg gagtagcctg tattatgagc gggttgatgc tgattttcct gcccacgaca
     7381 aaacaacgtt actgagtttt ccctgccact ttacactgcc gttcccactc atcggggcta
     7441 taacagcttg cagagaggac tttcaccgta tgcagctttt cacgattaat acaataaagc
     7501 gcattctgca aaggcgagag taaatccccc actttcacgg cgaagtcact cttcacttcg
     7561 gcgatcattt ttatcgcgcc gccagcatcg cagagcataa gtacatgtct gttatcttgt
     7621 ttattgcagc acgatttttt gaccagccat tccattgtat tatcctgttt gctaagagtt
     7681 ttcatatcaa tttacagatt aacagataaa ctctttatca acggcaatgg aatggcagaa
     7741 ctgagaagtg aaacggaggg ttaattattg ctgacgttgc acccactctt ttagcgcctg
     7801 acgtgaaatt ttaataccgc cgtttttcag ctccggcggc aaagtcagcc aacgtaccgg
     7861 ttgctgaaaa cgggctagct tatctttcac ccattcacta agatcaacgc tctcgtggtc
     7921 atactccatc accgccaccg gtcgatgacc aaactccttg tcggcaacgg ggacgataaa
     7981 cacctgcaaa accgcaggat gtgcagcaat tacgcgctcg acttcttccg gctgaatacc
     8041 ctctccgcca ctgaagaata gattgtctaa acgtccgaca atggtcagct tgccattatg
     8101 catctcaccg cgatcgcgcg tagcgtacca gccttcgtca ttaaccagtg aaaccagttg
     8161 cccgttacgc cagtaacctt ctgccatact ggcagcccgc agccacactt cattattaac
     8221 gattttcact tcccgacccg gcagcggcga accaacgtct gccaggccgt cggcttcttt
     8281 cgcacacacc gtggaggcaa actcggtcag accatagccg caaaagcaac gaatcccctg
     8341 ctcgcgcgcc tgttccgtca actcgaccgg gatagccgca ccgccaagta acaccgcttt
     8401 cagggaaacg gaactgcggt taaccagcaa acgccagagt tgtgttggca ccagtgaagc
     8461 gtgagtacag cctgccagca tttgctccaa tggctgttta tcacgcaccg tcatccgcgc
     8521 accagcgtat aaccagcgcc acataattcc ctgaccggag acgtgaaaca gcggtaaaga
     8581 gagcaaccag tcatcgtgat cgccaaacgg aatcaacgat aacacccctt cggcactggc
     8641 aagatgggcc tgataagtat ggacagcggc tttcggcaac ccggtagaac ccgaggtcaa
     8701 cgtcattgag cacagacgcg tcggctgcca cgcagcggca tgtgcgcctt caaccagctg
     8761 aatgtgcagc gacgttaacg ccggaaacgt gttttcccca tccggcacca gcgcaaattg
     8821 cagcgtcaga ttgggcagca attcttcaag caacggttgc ggcagctgag ggttcacggg
     8881 caacacccgc gccccgcatt gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt
     8941 ccacgcccgc aacatcacgc cgctgccctc aaccacaccc tgcaccgcaa atccggaggc
     9001 taattcatcg acgcgagcac aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa
     9061 ggcgatggct tctccccgca cttgccgcca gtgacgccac ggccagtcag agaagatcat
     9121 aacaaccgct ccagtgcatc aacttccacg acaggcagcg tgctacccgg ccagcgacgt
     9181 acctgctgcg cctgcatcag atccagcgtg tccagccctg gaatggtgtc cggcgttaac
     9241 caggcggcaa tccgcgccag ttgcgttaag cctaagctcg attcaatgga agaactgatc
     9301 accgccgtca gccccagcgc gtgcgccgcc tgtacctgct cgcgtacttt ttccagactg
     9361 cccgtgagcg tgggtttgat aactaccgcg cgcacgccct cttcagccac aaaggcaaaa
     9421 tccggcccgc gcaggctttc atcccaggca atggcaatgc cggtttcacg ggcaaacgct
     9481 cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg cgatgcggtc gcgataatcc
     9541 gggttaacgt atttggcaaa ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca
     9601 agacgcaaat gcagatccgg aattgcctcc agcaacagat tcaccaccat gccgtcgcgc
     9661 accgcttcgt acaatcccac tttgaccttc gccactttct cgcccggcat atcggcaagt
     9721 ttgaggatca gatcgtccgg atcgccatta cacagcggtg ccgcacggta gttggctgct
     9781 tgcggcaacg tatctgtcag ttctgccaat gcacagctta cgccaaaggc cacggaaggc
     9841 atctgcggta gctcgcaatc gcctgccagc cagttattta cccaggcaag cagcacactt
     9901 tgcgcctctt cccaggtttc ctgactgaag cccggcagtg gggagatctc cccccaccct
     9961 tcgcgctcgc cttcacgcag gcaaacatac agcccgtcgc gggtttttaa ccgcctgtcg
    10021 cgcagaacca cccccgcgtc catggggatc tgccagcggt atacctgcgc gctacgcatt
    10081 acggattccg tttgaatttg ctgaagtcag gctgacgttt ctggttgaag gcgttgcgac
    10141 cttcctgacc ttcttccgtc atgtagaaca gcatggtggc gttgcccgcc agctcctgca
    10201 gccccgcctg cccgtcacag tcggcgttca gcgcagcttt caggcagcgc agcgccatcg
    10261 ggctgttttg cagcatttcg cggcaccaac ggacggtttc tttttccaga tccgccagcg
    10321 gtaccacggt gttgacaagg cccatatcca gcgcctgttt tgcgtcgtac tgacggcaca
    10381 ggaaccagat ttcacgcgct tttttctgcc cgacgatacg agccatgtag gaagcgcccc
    10441 agccgccgtc gaaggaaccg actttcgggc cagtctgacc gaagatggca ttatctgccg
    10501 cgatagtcag gtcgcacatc atgtgcagaa cgtgaccacc gccgatggag tagccagcca
    10561 ccatcgcgac aaccggtttc ggacaggtac ggatctgacg ctggaagtcc agcacgttca
    10621 ggtgatgtac gccggaatca tctttatagc cgccgtaatc accacgcact ttctggtcac
    10681 caccggagca gaacgcttta tcgcctgcgc cagtcagaat gatcacgccg acgttatcgt
    10741 cataacgcgc atctgccagt gcctggatca tctcttttac cgtcagagga cggaaggcat
    10801 tgcgcacctg cggacgatta atggtgattt ttgcgatacc gtcggtggat ttttcataac
    10861 gaatgtcctc gaaaccttcg gagcagtcgt gccattcaac cggtgcgtaa agcattgctt
    10921 catcaggata aatcatattg tgtcctttag tcagaaacgc aagatctgcg ccagacttgc
    10981 gattacgcca gcgggatttt cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca
    11041 gtcggcagcc agttccgccg ccagggcgcg gaatttgctg tcacgttcac cacataaata
    11101 ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga
    11161 agtcgcctcc agcatggcgg caagcgttgc gccattattg ttgctgcgca gcgccaccag
    11221 ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa
    11281 taccgcggtt aatggttcgg aacgaaaacg ctgcgcccat tggcgatcgg aacgctgacg
    11341 ttccgcgcgt tgttcagcat tttgcagccc cggatgaccg ccttcgacaa caaccccaca
    11401 aagccccgcc agcctctggc aagccgccat catcgccacc cgtccgccaa gcgagtaccc
    11461 caccagccag aagttaagga tgttgtaact aaccagggtt ttacgcagta agtcggtgac
    11521 atcatcaaat ccatcgacgc taatcgccgc cgaaccaccg tgacctggga gatcaacata
    11581 caaccgtgag tagtcggcaa acacctcgcc cacttcttgc cattcgtggc aatcgccgga
    11641 aaaaccgtgc aaaaacacca gccagggtaa acctggtttg ccgtgttttg cctgcgcgtg
    11701 caggatcata aatggcttac ctgcgccaga agttgctgga gcgtttgcgc gccgtcggtg
    11761 tcgttaacca ccatttcaat taccgtggtg gttggcgtgc gccaggcatc ggcaagtgcc
    11821 gtttcaagtt cctgccagtt ttgcggacga tgatatttca gctcgaacat cgcggcggcg
    11881 tgctcaaaat ggacgttttg cggcatcaga tagaaacgct cgcgctcgct tttcggcgtt
    11941 ggcagcagcg agaaaatttg cccgccgttg ttgttcacca caattaatac cagcggcgcg
    12001 gaaacctggc gcaataacgc cagcgcattc aggtcgtaaa gtgcggagag atcgcccaca
    12061 atcgccagcg tcggtttgcc gcttgcccgc tgaacgccgg cggcagtcga gagcagcccg
    12121 tcgataccgc tggccccacg gttgctgtac accgggtaac ctgccggaag ttgcgaaagc
    12181 gcatcaatca gacgtaccac caggctgtta ccgacaaaca attgcccctg ttcaggcaga
    12241 tagtcgcaga cgcgatgcgc cagttgcgct tcgccaaacg catcgcgacg ggcaataacc
    12301 gcctgcatcg cctgctccgc caggcgcggg atttcaacgc accagggctg gcgtttttct
    12361 gccggatgca gttccagcca gtcggcaata ttggcaatta accgacgccc gcgatggtgc
    12421 gccggatcaa gccgcccttc gatgtcatcc acaatccagt actcttcggg ttcacagctt
    12481 gcctgccatt gcaggagccg tttgccagtc aggctgcttc ccagttgcac cacaatttgc
    12541 gcctgttgca gttcgctggt cgctttggca ttgcctaacc agagatcggc acacggcagc
    12601 ggctgtccgg tttgcgacag cacgtcgcca atcagcggcc agccaagagt ttgcgcccac
    12661 agtgcaactt ttttgccctc ttccgcactc atgcgcccgg caaccaccac gccgcgtttt
    12721 tgccgccaga agaaccagtc gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc
    12781 cacggcttat cgtcctgcca ccagtcgccc agacgctgtt gccagctaag cccggtatcg
    12841 tccatttcgc catacagcgg ttcagcaaac gggcagttga tatggactcc acccgcatga
    12901 agcgtaccga gagcgtggtc gatggtagaa accagccaac gtgcggggat atcctgggtc
    12961 gggcgcggca atgaaatact gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt
    13021 gcctgattcg cgccgcagtc aattagctcc ggcgggcgat cggcggttaa gagaatcagt
    13081 ttttctccgg ttaacccggc ttcaatcagt gccggataga gatttgccac cgccgtaccg
    13141 gaggtgacaa tcaccgccac cggctgcttg ctgactttcg ccagccccag cgccagatgc
    13201 cccagtccac gctcatcgaa atgggtgtgg tgaatgaatg cggaattctc cgccgccgct
    13261 aacgttaacg gtgtagaacg cgagccaggg gcgatacaga tgtgtcttac gccgtgacgc
    13321 gttaatgctt ccagaatgac cgccgcccag cgtcggttaa atgcgcttac tgacatgagt
    13381 ttgtccggta tcaatattgc ggctaagtat aaggagctac aaaaatagat tattgatatg
    13441 aatcggtaat gatgcgactc attactattc catttgtaat aaagtacgca gccctgccgc
    13501 tttgttgtcg atttcctgcc actcttgctc ggggtcggag ccacggacaa tgcccgcgcc
    13561 agcatatagc cgcacgacat tgccgctgat ttttgctgag cgcagggaaa cacagaattc
    13621 gctttgctgt aatgagagat agcccgcaga accggcgtac cattctcggg tgaacggttc
    13681 gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg
    13741 ctgcaactga tgtaaacaga tcacatcatc cgctttgttg agtgaagtcc agatacagcg
    13801 gcgaagatgc tgcactttac gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg
    13861 ggtatcggcc tgtaatcgtt ggcagatgtc ttccaccacc agcatgttct cgcgctggtt
    13921 tttatcatcc gccatcagcc actctcctaa ctgctgcgcc tgcttatcat caggatgatt
    13981 tgctactgtt cccgccagcg cttcagtacg cagcgctttg tcacgccgcc gccataaccg
    14041 ttccggtgaa gagccaagaa aagcattttc gccatcaaag gccatgtaaa aatggtagca
    14101 attgagattc agtcgacgac tggccgccat catcgccgcc gcattgaccg gacttgcgaa
    14161 atgcaggtca gttgcccgag caaacaccac tttgtcgagc tcaccttcgg cgatggtttt
    14221 cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt
    14281 agtggttaaa tgtaacccag gcaagggctt gatactcacc agtgtggcga taaattctct
    14341 tgccttaatc gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc
    14401 tttaccgcca cagcgtcgcc attccaggcg tggtaaaagt aaattgccct gcgacgggtc
    14461 aaacgcattc agcccccaaa tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg
    14521 ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc aggacggcag cttcttcatc
    14581 accattacgt tgttgccagt agaattgcgg gtacgtttgc tgactggcca gccagctcaa
    14641 ggcatcaaaa gcgtctttga gagggaaagg aatatcgata acccgaatgc cgggtgtcgc
    14701 cggaatctct tgcgacaaat ggcgcagtag attttccagc gccgtagtaa gtgattgcac
    14761 gcggacctct ccctgttaaa agcctcacat tatacggggt actacaaaaa aaatgcagta
    14821 ccccggtgta gggaggtttt aacggcgtgc cagcaacagt cctagtacca gcccaacggc
    14881 cgcacccaca ccaattccct gccagggttt ttcgtggacg tagtcatcag cacgataaac
    14941 agcctgcttc gcccgatagt aataactgtc tgaagcctgg ctaacccgtt ttttcacatc
    15001 atccagcgct ttttctgcac gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc
    15061 agaggagcgg agcacctctt ccagtgtttc actaagcagc gtcaggtcgt catcgatacg
    15121 tgtatcacca aactgattag acattctcgt tctccatttg cgtaaaacct gtcactaact
    15181 atagacaagg gttattgatt acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat
    15241 accatcttct tcatagatct ccgtcacagg gataaaaccg aaactctggt agaagttttg
    15301 cagatgcgcc tgcgccccga ggtagacagg cttatcaggc cagtgatgcg tgcaagtgtc
    15361 gagggtttta ctcatcagtt gctgccccac tttttcgcca cgcaacgctt cactgacaat
    15421 cacccgacct ataacgaccg gctcaagatc atcatcactt ttcagaatcc tcgcatacgc
    15481 caccagttca tcatttttcc aaccgagaat atggcgatta tcccctgtca aatcatcacc
    15541 atcgatatcc tgataagggc aattctgctc gacaacaaac accgcgcaac gcagttgtaa
    15601 taaggcataa agctgggaaa ccgataattc agagtgatgc agatcttgcc attcaatcat
    15661 aatgctctcc ttttagatgt atgccttata ctagccggaa tattttttga aacgtgatga
    15721 actcatggaa ttaatttttt taggtacttc ggccggtgtg ccaacccgta cgcgcaatgt
    15781 cacggcaata ttgcttaacc tgcaacatcc gacccagagc ggtctttggt tgtttgactg
    15841 cggtgaaggc acccagcatc agctactgca taccgccttt aaccctggaa aactggacaa
    15901 gatttttatc agtcaccttc atggcgatca tctctttggt ttacccggct tgctgtgcag
    15961 tcgttctatg tcaggcatta tccaaccctt aacgatttat ggtccgcaag gtatccgtga
    16021 atttgtggag accgcgctgc ggattagcgg ctcatggacc gattatccgc tggaaattgt
    16081 cgaaattggc gctggcgaaa ttctcgatga tggcctgcgc aaagtgaccg cttatccaat
    16141 ggaacaccca ctggaatgtt atggctatcg tattgaagaa catgataaac cgggtgcatt
    16201 aaatgcccag gcattaaaag ctgctggcgt accgcctggc ccactatttc aggaattaaa
    16261 agcgggcaaa acaatcatgc tggaagatgg aaggcagatt aacggcgcag attacttagc
    16321 cgctccagtg ccaggtaaag cgctcgctat tttcggcgat accggcccct gcgatgccgc
    16381 acttgacctg gctaaaggtg tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat
    16441 ggaagccaaa gccaatagtc gcggtcatag ctctacacgc caggctgcga cactggcccg
    16501 tgaggctgga gtcggtaagc taatcattac ccacgtcagc tcgcgctatg atgataaagg
    16561 ttgtcagcac ctgttacgtg aatgcaggtc aattttcccg gcgactgaac tggcgaatga
    16621 tttcaccgtg tttaacgttt aactctgctt attattaaaa acagggcgaa acttgccctg
    16681 ttatcgcaac ccgcgctaca tatatccacc cacacgggca tattgagcga tttttgatat
    16741 ttattcatct ccttacgcac tatattcaga gcaataagtt gcctcttttt atcttcattc
    16801 agcactacct ttgcaactaa aaggagcata tgatggttac agttgtcagc aattattgtc
    16861 aattatctca aacccaactc agtcagacat ttgcagaaaa atttactgtg accgaggaat
    16921 tactacagtc tttaaaaaaa acagcgttat ccggagatga agaaagtatt gagttactgc
    16981 ataacattgc gttaggttat gatgaatttg gaaaaaaagc tgaagatat
//
LOCUS       DAZGBC010000102        16951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGBC010000102 DAZGBC010000000
VERSION     DAZGBC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.103"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            461..613
                     /gene="mokC"
                     /locus_tag="ACHK1J_003277"
     CDS             461..613
                     /gene="mokC"
                     /locus_tag="ACHK1J_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO7912512.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(717..1847)
                     /gene="dnaJ"
                     /locus_tag="ACHK1J_003278"
     CDS             complement(717..1847)
                     /gene="dnaJ"
                     /locus_tag="ACHK1J_003278"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO7912513.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1936..3852)
                     /gene="dnaK"
                     /locus_tag="ACHK1J_003279"
     CDS             complement(1936..3852)
                     /gene="dnaK"
                     /locus_tag="ACHK1J_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO7912514.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4229..4633
                     /gene="yaaI"
                     /locus_tag="ACHK1J_003280"
     CDS             4229..4633
                     /gene="yaaI"
                     /locus_tag="ACHK1J_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO7912515.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4659..5372
                     /gene="msyB"
                     /locus_tag="ACHK1J_003281"
     CDS             4659..5372
                     /gene="msyB"
                     /locus_tag="ACHK1J_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7912516.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5521..6087
                     /gene="satP"
                     /locus_tag="ACHK1J_003282"
     CDS             5521..6087
                     /gene="satP"
                     /locus_tag="ACHK1J_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO7912517.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6122..6709)
                     /gene="mog"
                     /locus_tag="ACHK1J_003283"
     CDS             complement(6122..6709)
                     /gene="mog"
                     /locus_tag="ACHK1J_003283"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO7912518.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6824..7777)
                     /gene="tal"
                     /locus_tag="ACHK1J_003284"
     CDS             complement(6824..7777)
                     /gene="tal"
                     /locus_tag="ACHK1J_003284"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7912519.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8056..9486
                     /gene="yaaJ"
                     /locus_tag="ACHK1J_003285"
     CDS             8056..9486
                     /gene="yaaJ"
                     /locus_tag="ACHK1J_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO7912520.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9556..10332
                     /gene="yaaA"
                     /locus_tag="ACHK1J_003286"
     CDS             9556..10332
                     /gene="yaaA"
                     /locus_tag="ACHK1J_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO7912521.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10485..10781)
                     /gene="yaaX"
                     /locus_tag="ACHK1J_003287"
     CDS             complement(10485..10781)
                     /gene="yaaX"
                     /locus_tag="ACHK1J_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO7912522.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10995..12281)
                     /gene="thrC"
                     /locus_tag="ACHK1J_003288"
     CDS             complement(10995..12281)
                     /gene="thrC"
                     /locus_tag="ACHK1J_003288"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO7912523.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12282..13214)
                     /gene="thrB"
                     /locus_tag="ACHK1J_003289"
     CDS             complement(12282..13214)
                     /gene="thrB"
                     /locus_tag="ACHK1J_003289"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO7912524.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVETAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDARLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13216..15678)
                     /gene="thrA"
                     /locus_tag="ACHK1J_003290"
     CDS             complement(13216..15678)
                     /gene="thrA"
                     /locus_tag="ACHK1J_003290"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO7912525.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15704..15823)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15759..15824)
                     /gene="thrL"
                     /locus_tag="ACHK1J_003291"
     CDS             complement(15759..15824)
                     /gene="thrL"
                     /locus_tag="ACHK1J_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO7912526.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16038..16724)
                     /gene="yjtD"
                     /locus_tag="ACHK1J_003292"
     CDS             complement(16038..16724)
                     /gene="yjtD"
                     /locus_tag="ACHK1J_003292"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7912527.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3970 a         4464 c         4193 g         4324 t
ORIGIN      
        1 cagcggtgag atgcgttttt gacggctgta agcgctgacg ggtgacccta agccctgata
       61 atcatagctt aaggtgagga tttctgaggg attaattagg aaaagtaata attaatttgt
      121 tgtcagtcgg cgatgtttgt taggccaata tttcaaaaat aatccagctt gtcgtcaata
      181 attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat tacgccataa
      241 aaatgcgagg cgtcttccgg attagaatcg ataaaacaga atgggttaaa gagagcgata
      301 taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg cagatagaga
      361 aaagccccga gtgatatttt accatcaacc cgaggccccc tatatgctga acacatgtag
      421 agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc ataaggcgat
      481 gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg taacgagaaa
      541 agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt tcacggctta
      601 cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg tgctcgcata
      661 tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg gggaggttag
      721 cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga gcgcgggctg
      781 ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag ctgtttctgc
      841 ttctcgttca ggcctacagg tgtttcgacg acaacgcggc acagcaaatc accctgtgcg
      901 ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc ggtctgggtt
      961 tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat ttcgccaccc
     1021 agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc ttcacgctcg
     1081 aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg tgcgccatgc
     1141 tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac ccctgccggg
     1201 attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca tttgttgcac
     1261 ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg cacggcaaag
     1321 aaaccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt ctgcggctgt
     1381 gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt cggaatgcgg
     1441 atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt atagcgtaaa
     1501 tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc gccgaaaacg
     1561 tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac cgccgccgcc catgccacct
     1621 tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga gtcggtcaga
     1681 acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc accctggtta
     1741 cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttttgatttc acgctcttcc
     1801 gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt ttttaaattg
     1861 cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca gtataattac
     1921 ccgtttacag ggcgattatt ttttgtcttt gacttcttca aactcagcgt cgacaacatc
     1981 gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct gggcatgttg
     2041 ctgctgggcg atttccatca gtttctggga aacctgtgcc agctcctgca ttttcgcttc
     2101 gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg cagactcgat
     2161 agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct tacgggtgct
     2221 gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact tacggtcagc
     2281 ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt tcagaccaga
     2341 agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg cggaaacgtg
     2401 caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatac cgcgcggtgc
     2461 cgggttgata ccatccaggt tgaactgacc cagagatttg ttatcagccg cacgtttacg
     2521 ttcaccctgc agcacgtgga tggttaccgc agactggttg tcttcagcgg tagagaacac
     2581 ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca tcacaccgcc
     2641 catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt cttttacgtc
     2701 accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt ccgggttaac
     2761 gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca ttggcatacg
     2821 agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc cagcgtcctg
     2881 cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca ggctttccag
     2941 tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg cagtgatgta
     3001 cggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt tttctgccgc
     3061 ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct gatctttctt
     3121 gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac ccaggtgggt
     3181 atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt cgatttcaat
     3241 aatagaaata tcgaaagtac caccacccag gtcataaacc gcgatagtac ggttgccagt
     3301 acctttgtcc agaccgtaag ccagcgcagc tgcagtcggt tcgttgatga tacgttttac
     3361 ttcgagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat cgttaaagta
     3421 tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt cagcggtttt
     3481 cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct ggcctttaac
     3541 ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga tggaaacatc
     3601 acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg caaacagggt
     3661 gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag tttcaccatc
     3721 ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct ccagcacacg
     3781 aggagtggtg ccatccataa tcgctacaca agagttggta gtacccaggt cgataccaat
     3841 tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt gtgagtctgt
     3901 aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag actcactcac
     3961 tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat gcaaaaattt
     4021 tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa ttgacagcaa
     4081 ttttattcat ataaagaatg aatcgcagtc aaggtattca tcaaaaaatt gtaataaaaa
     4141 gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg tattcatccc
     4201 cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt ccgccagcct
     4261 ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac tcctcggcgt
     4321 cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg tttgtcgcat
     4381 tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagcg gtgcatcggt
     4441 ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag aaataaaaga
     4501 agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac gctgcgtctc
     4561 aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc tgaaaattat
     4621 cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat tacctgaatg
     4681 attcagatct ggattttctc cagcattgta gtgaggaaca gttagcaaat ttcgcccggc
     4741 tgctcaccca taatgaaaaa ggcaaaactc gcctctccag cgtactgatg cgcaacgaac
     4801 tgtttaaatc gatggaagga catcccgagc aacatcgccg caactggcag ctgattgccg
     4861 gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat
     4921 tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc gacaaagaga
     4981 tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga
     5041 aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc
     5101 tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt gtgtcgcatt
     5161 tgctttccag ccaattgacc cgcattttac gcacccacgc agcaatgagc gtgcttgggc
     5221 atggtttgct gcgcggcgcg ggtctgggag gccctgtggg cgcagcatta aatggggtta
     5281 aagcggtcag cggcagcgcc tatcgcgtga cgattccagc cgtactgcaa atcgcctgcc
     5341 tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa attttcattt
     5401 tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa
     5461 cactacagtt attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt
     5521 atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc
     5581 accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat tattcttgcc
     5641 atgggcattt tctacggcgg catcgcgcaa atttttgccg gtctgctgga gtacaaaaaa
     5701 ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt
     5761 gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca gttccttggt
     5821 gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac gctgaaaggc
     5881 gcacgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc
     5941 ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg tctgatctgc
     6001 ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt tggtcgcacc
     6061 gttctgccga ttggtgaatc ccactaattg ttttgagatc cccccgctcg gggggatttt
     6121 tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc acttctggtg
     6181 ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac
     6241 cgtgtaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct ttaatagact
     6301 tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacaccc acctgacgcg
     6361 aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagccag
     6421 gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgccgggc
     6481 cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc tcacacaacg
     6541 tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca aacggcgtgg
     6601 ttagcgccga tgtcagccat tcttccagcg cagggatgcc tttatcctga taaacgccac
     6661 tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc
     6721 cgtttaaaca gtctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggcttc
     6781 agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc agatcgccga
     6841 tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc gccagtttat
     6901 ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg atacgcgccg
     6961 gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct tcgctctcag
     7021 ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc gcagcctgcc agttccagaa
     7081 tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg tgctctttgt
     7141 agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga gcgtactctt
     7201 tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaatgga gagatcagaa
     7261 acacgcccgc ttccgcacaa gcacgagcct gagcgaagga gaacagcagg gtcaggttac
     7321 agttgatacc ttctttttcc agctgttccg cagcacggat accctgccag gtagaagcca
     7381 gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg atcaggcgtt
     7441 ttgctttcgc aattgacgct tcggtgtcat aggaaagacg cgcatcaact tcagttgaga
     7501 tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt ttgtcagtcg
     7561 cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat
     7621 caatcaactt acgatattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg
     7681 tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg gccactacgg
     7741 tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc
     7801 ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc
     7861 gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact gcgttgatat
     7921 cgcccgccat ttttaaacaa aacctcatgt atgctacgca gaagttatca agtacctcgt
     7981 agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca
     8041 ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg
     8101 gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac cggatttgtg
     8161 cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca
     8221 ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt gggtagcggc
     8281 aacctggccg gcgttgcgct ggctattacc gccggtggac ctggagccgt cttttggatg
     8341 tgggttgccg cgtttatcgg catggcgacc tcgtttgccg aatgttccct ggcacaactt
     8401 tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc
     8461 gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat cgcctacggc
     8521 ataattttca gcggagttca ggcaaacgct gttgcccgag ccctgagttt ttcttttgat
     8581 tttcccccgc tggtgacagg cattattctc gctgtctttg ctctgctggc aatcactcgc
     8641 ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc gataatctgg
     8701 gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca cgtcatttgg
     8761 tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc
     8821 ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga ggcgggaatg
     8881 ggttcgacgc caaacgcggc agcggcagcg gcgtcctggc ctccgcatcc ggcagcgcaa
     8941 ggaattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc
     9001 atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt
     9061 atccagaagg cgatgcgggt gctaatgggt tcttggggtg ctgagtttgt taccctcgtg
     9121 gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc
     9181 ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg caccttcgca
     9241 acggtcatcg gcggcacctt gctaagtctt ccgctgatgt ggcaactggc agatatcata
     9301 atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc tgtggttcat
     9361 accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg
     9421 ttgcgttatc cggatatcgg tcgccagctt tctcgggacg cgtgggatga tgtttcgcag
     9481 gagtaatcac aactatcgat caactcattc tcgttttttg ctaaagtcgg cataaatttc
     9541 ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga ttaccaaagc
     9601 ccgttgacca ccacgcgata tacactgccg gagctgttag acaattccca gcagttgatc
     9661 catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgtat cagcgacaaa
     9721 ctggcaggta tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggagaat
     9781 gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc
     9841 ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat
     9901 ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt
     9961 gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg
    10021 aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat
    10081 tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat
    10141 gagaagaacg gcaaatttaa gataatcagc ttctacgcta agaaagcacg cggtctgatg
    10201 agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc
    10261 gaaggttact tctttgatga agcttcctcc agcaatggcg aactggtgtt taaacgctac
    10321 gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg gacgccttat ccggcctaca
    10381 aaagcatgca gattcaatat attgcagaat tcacgtaggc ctgataagac gcgccagcgt
    10441 cgcatcaggc gctaaatgca ggattgttac ccggcatttg tcatttagcg gtgatgtttg
    10501 cctggaccat gaccgccgtg atgatcatga tgagctttct tatggtgacg cggcggtggc
    10561 ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt ccaccagccg
    10621 tggtcgcgcc agtgaccgcc atcccagtaa taaccgcgat tatcacgatc gcctatctgt
    10681 aattttactg acgggactaa cgtaatttcc gcagcctgcg ctgccatggg agcgaccaga
    10741 accagggaaa gtgcgagtac aatagattgc atctttttca caggttactc cttttatcat
    10801 tgcccgttat gggtcgatat ccggagatta ctcgatgaga acggcggttg ttattctccg
    10861 caatcctaat ctctaagtta ccgcatttat tgagaatttc tcctttttcc ctgtcgtttt
    10921 tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg cctgattgag
    10981 ataatgaata gatattactg atgattcatc atcaatttac gcaacgcagc aaaatcggcg
    11041 ggcagattat gcgaaagcaa gggtaaatcg gcacgttctg ccagctcttt tggcagatcc
    11101 aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg cgcggtgccg
    11161 aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata cgctacggca
    11221 gcgtgcggct ccgaagtgta gcccagttct tttaactcac gcattgtctg ttgcgtggtt
    11281 tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg gcggaacaac
    11341 tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc
    11401 tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt atcgttcacg
    11461 ttggtcgcag caataaaacg tttcaccggc agaccgagtg acttcgccag cagacccgcc
    11521 gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg cgcttcctgc
    11581 ggcagctgcg caactgcttc aaagtagtag caaatctgcg ccagcaaccg gctgatgtta
    11641 atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc aaacgcctgc
    11701 ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc gatattgccg
    11761 cccaatgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg atagaggata
    11821 accactttca cattcggtaa accgtagaaa gcatgagcca ctgccgctcc ggtatcaccg
    11881 gaggtcgcgg tcagaatggt cactggctta tcgcccgcaa tatgggtcag catttgtgcc
    11941 ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa ttccagacaa
    12001 ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc gcgcacgcgc
    12061 tcttccagga tttcctgcgg gatttcatca ccaataaacg ccgagaggat cttcgcactg
    12121 cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa ttccggcagg
    12181 tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc ttgcgcaaag
    12241 ctgacctgct cattgtgatc tttcagattg tagagtttca tttagttttc cagtactcgt
    12301 gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg caggtagttc
    12361 ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacacag agcgaacaag
    12421 gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac cgcctgccgc
    12481 gcctgccgga agccaggcag taaccgttca cggtagggtt ctgcgataac atctttcatc
    12541 agcttcgcgg caagctcagg ctgacgggaa tagcaagcgt gaatgaagcc tgccagatgt
    12601 cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc ccgggcttct
    12661 gccgtcgaga ctttaatccc cggatacgcc agcacccaca gccactcatc aaaccctggc
    12721 acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc aagaaaacac
    12781 ggtgccacgt tgtcgtaatg aatgctgccg gagatacgcc cttccagctc gcccatcaaa
    12841 gccagcaaac gagcgtcatt aagcggcttg ccgcagtgtt cattcatcgc catcagcgcc
    12901 gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt cttttccaga
    12961 gtcatcgcca ctggaatttg cttgcccagc tcctggcaaa aacgctccca gcactgataa
    13021 acgatatttt cccgtggttc tgacggcagc ttatcggcaa aacgtccgag gttgttgagg
    13081 ctgaatgtct ctgccgtctc aaccgtgact acatctccga gcaatgcacc atcaacgggt
    13141 gtcaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga agccggggca
    13201 taaactttaa ccatgtcaga ctcctagctt ccatgagagg gtacgtagca gatcggcaaa
    13261 gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca atggcagcgg
    13321 ctgataatag tggctataga aggccagggc gttttcgcca tttttcactt tgaacagcgg
    13381 atcattacca tccacttcgg caatcttcac gcggcagacg ccatcttcat caatattgcc
    13441 aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc
    13501 gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt taaactctgc
    13561 gggcagcact ggttcaattt caatatccgc caactccagt tcacgtcccg tttcacgagc
    13621 gagaattaat agtttacgcg ctacatccat accagaaaga tcatctcgcg gatccggttc
    13681 ggtataaccc atttcccgcg ccagagtagt cgcctcggag aaactcatgc cttcgtctaa
    13741 cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca tcaattcatc
    13801 accagcattg agcagatttt gcaggttctc aataaccggt aatccagccc caacgttggt
    13861 gtcatagagg aatttacgtc gcgatttttc cgccgcatga cgcaacagat ggtagtaatc
    13921 catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt cgcgcaggaa
    13981 gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga ccgggttcag
    14041 cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg
    14101 cgccagttct tcctgccagt tttccaggtt taggccatgc acattggtaa gcagagcctt
    14161 cgagttagca acaccgcaga cacgtaagtc gatatgttta ttcttcaacc agctttgctg
    14221 acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca caaacacttc
    14281 gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag tggtcgcatc
    14341 atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa tggcgacaat
    14401 gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg
    14461 cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca gcggctccag
    14521 taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag ctcgcacaca
    14581 gtcgctttgc ggaacgcaga aactgatact gtattcggaa gatgattgcg taatcagcac
    14641 cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc cgaccattcc
    14701 tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg aaatgccctt
    14761 gaccggtaat tcgtcttcat cacgactggc accaatgagc gtacctggtg cttgaggatt
    14821 tccggtattt ttaatcaggc acgggatctg gaactgggcg atgggggtaa tggtgcgggg
    14881 gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg acatcgattt
    14941 caacaacctc gcatcgggca cctgacgcgg gtcgcaggta taaaccccgt caacgtccgt
    15001 ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg agtagtcgga
    15061 accgttgcgt ccaagtacca ccagttcgcc tttttcatta ccggcggtga aacctgccat
    15121 cagcaccatg tgatcagccg gaatacgact tgccgcaata cggcgggtgg actcagcaat
    15181 atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg gatcgataac
    15241 ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgattg acattttctc
    15301 gccacggcaa atcagcgcag cgttaatgct atccgggcac tgccctaaca aactaatgcc
    15361 atgcaggaca tgttttattt gggcaaattc ctggtcgacg aaagttttca attgcgccag
    15421 cgggaatccc ggctgggcgg cggcgagtcc cgtcagaagt tcggcaaaaa tacgttcggc
    15481 atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca tcgccaccag
    15541 gtggttggtt attttggcgg gggcagagag gacggtggcc acctgcccct gcctggcatt
    15601 gctttccaga atatcggcaa cccgcagaaa acgttctgca tttgccactg atgtaccgcc
    15661 gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa aagcccgcac
    15721 tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg
    15781 gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct
    15841 gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag tgacctaagt
    15901 caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgctg tcagagactc
    15961 tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat
    16021 acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc
    16081 aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gttgtaacca gtcgaccagt
    16141 tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tggctcgttc gcgtaaagct
    16201 tgcagttgat gttggtctgc cgttgtgtca cttttcgtcg gttgttgtat taatgttgct
    16261 aattgatagc aatagaccat caccgcctgc ccaagattga gcgaaggata atccgccacc
    16321 atcggcacac cagtaagaac gtcagccaac gctaactctt cgttagtcag cccggaatct
    16381 tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc
    16441 accagttcta ctggcgtggc gtagtaatga tatttcgccc gactgcgtgc agtggtggcg
    16501 acagtgaaat cgacatcgtg taacgattca gccagcgtcg ggaaaacttt aatattatca
    16561 ataatatcac cagatccatg tgcgacccag cgagtggctg gctccagatg tgcctgacta
    16621 tcgacaatcc gcagttcgct aaaccccatc gttttcattg cccgcgccgc tgccccaata
    16681 ttttctgctc tggcgggtgc gaccagaata atcgttatac gcatattgcc actcttcttg
    16741 accaaatgac cgcgaacagg gtgatcactg ccaacttatt acgcggtgcg aatttacaaa
    16801 tccttaacgt aagtcgcaga aaaagccctt tacttagctt aaaaaaggct aaactatttc
    16861 ctgactgtac taacggttga attgttaaaa aatgctacat atccttctgt ttacttagga
    16921 taattttata aaaaataaat ctcgacaatt g
//
LOCUS       DAZGBC010000103        16946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGBC010000103 DAZGBC010000000
VERSION     DAZGBC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.104"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..1171
                     /gene="ompN"
                     /locus_tag="ACHK1J_003293"
     CDS             38..1171
                     /gene="ompN"
                     /locus_tag="ACHK1J_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO7912528.1"
                     /translation="MKSKVLALLIPALIAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1312..1746
                     /gene="uspF"
                     /locus_tag="ACHK1J_003294"
     CDS             1312..1746
                     /gene="uspF"
                     /locus_tag="ACHK1J_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO7912529.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1922..2857
                     /gene="ttcA"
                     /locus_tag="ACHK1J_003295"
     CDS             1922..2857
                     /gene="ttcA"
                     /locus_tag="ACHK1J_003295"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO7912530.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2986..4359)
                     /gene="dbpA"
                     /locus_tag="ACHK1J_003296"
     CDS             complement(2986..4359)
                     /gene="dbpA"
                     /locus_tag="ACHK1J_003296"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO7912531.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQQLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGYIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4389..4562)
                     /gene="ynaL"
                     /locus_tag="ACHK1J_003297"
     CDS             complement(4389..4562)
                     /gene="ynaL"
                     /locus_tag="ACHK1J_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO7912532.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4837..5820)
                     /gene="zntB"
                     /locus_tag="ACHK1J_003298"
     CDS             complement(4837..5820)
                     /gene="zntB"
                     /locus_tag="ACHK1J_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO7912533.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5834..5881)
                     /gene="ydaN"
                     /locus_tag="ACHK1J_003299"
     CDS             complement(5834..5881)
                     /gene="ydaN"
                     /locus_tag="ACHK1J_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO7912534.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6074..7306
                     /gene="dgcM"
                     /locus_tag="ACHK1J_003300"
     CDS             6074..7306
                     /gene="dgcM"
                     /locus_tag="ACHK1J_003300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO7912535.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLVA"
     gene            complement(7327..7890)
                     /gene="smrA"
                     /locus_tag="ACHK1J_003301"
     CDS             complement(7327..7890)
                     /gene="smrA"
                     /locus_tag="ACHK1J_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO7912536.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8220..9128)
                     /gene="abgR"
                     /locus_tag="ACHK1J_003302"
     CDS             complement(8220..9128)
                     /gene="abgR"
                     /locus_tag="ACHK1J_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7912537.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVREILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9304..10614
                     /gene="abgA"
                     /locus_tag="ACHK1J_003303"
     CDS             9304..10614
                     /gene="abgA"
                     /locus_tag="ACHK1J_003303"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO7912538.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10614..12059
                     /gene="abgB"
                     /locus_tag="ACHK1J_003304"
     CDS             10614..12059
                     /gene="abgB"
                     /locus_tag="ACHK1J_003304"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO7912539.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPISKNVTPSPLK"
     gene            12096..13622
                     /gene="abgT"
                     /locus_tag="ACHK1J_003305"
     CDS             12096..13622
                     /gene="abgT"
                     /locus_tag="ACHK1J_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO7912540.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13633..14148
                     /gene="ogt"
                     /locus_tag="ACHK1J_003306"
     CDS             13633..14148
                     /gene="ogt"
                     /locus_tag="ACHK1J_003306"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO7912541.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14343..15095
                     /gene="fnr"
                     /locus_tag="ACHK1J_003307"
     CDS             14343..15095
                     /gene="fnr"
                     /locus_tag="ACHK1J_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO7912542.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15247..16197
                     /gene="uspE"
                     /locus_tag="ACHK1J_003308"
     CDS             15247..16197
                     /gene="uspE"
                     /locus_tag="ACHK1J_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO7912543.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16247..16504)
                     /gene="ynaJ"
                     /locus_tag="ACHK1J_003309"
     CDS             complement(16247..16504)
                     /gene="ynaJ"
                     /locus_tag="ACHK1J_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO7912544.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16748..>16946
                     /locus_tag="ACHK1J_003310"
     CDS             16748..>16946
                     /locus_tag="ACHK1J_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO7912545.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIE"
BASE COUNT         4325 a         4205 c         4123 g         4293 t
ORIGIN      
        1 gataaaaggc aaataaaata actaaaggat ttattcaatg aaaagcaaag tactggcact
       61 tttaattcct gccctgatcg ccgcaggtgc tgcacatgca gccgaagttt ataataaaga
      121 cggcaacaaa ttagatctgt atggcaaagt tgatggcctg cattattttt ctgataattc
      181 agcgaaagat ggcgaccaga gctatgcgcg tctgggtttt aaaggcgaaa cccaaattaa
      241 cgatcaactt actggctacg gtcaatggga atacaatatt caggcaaaca acactgaatc
      301 ttcaaaaaac cagtcatgga cccgtctggc atttgccgga ctgaaatttg cagattacgg
      361 ttctttcgat tacggacgta attatggcgt aatgtacgac atcgaaggct ggaccgatat
      421 gctgcctgaa tttggcggtg actcttatac caatgcagac aactttatga ctggtcgtgc
      481 caatggcgtc gcgacttatc gtaatactga tttcttcggt ctggtaaatg gtctgaactt
      541 cgcggtgcag tatcaaggta acaacgaagg tgccagtaat ggtcaggaag gcaccaacaa
      601 cggacgtgat gttcgccatg aaaacggtga cggctggggt ctttccacaa catatgattt
      661 aggcatgggc tttagcgctg gtgcggcata cacctcttct gaccgcacca atgaccaggt
      721 taaccatact gcggcgggtg gtgataaagc agacgcgtgg actgctgggc taaaatacga
      781 tgctaacaat atttacctgg caaccatgta ttcagaaacg cgtaatatga ccccgtttgg
      841 cgacagcgat tatgcagttg cgaacaaaac ccagaacttt gaagtcactg cacagtacca
      901 gtttgatttt ggtctgcgtc cggcagtctc tttcctgatg tctaaaggcc gtgacctgca
      961 cgctgccggt ggtgcagaca acccggcagg tgttgatgat aaagatctgg ttaaatacgc
     1021 cgatgttggc gcgacttact atttcaataa aaacatgtcc acttatgttg actataaaat
     1081 caacctgttg gatgaagatg acagtttcta cgctgccaac ggcatctcta ccgatgatat
     1141 tgtcgcttta ggtctggttt atcagttcta aatcctcctg cccgctgtta tggcgggctt
     1201 tttctgctta ttcttcctcc tttgatctaa attaaaaatg tgaactccgt cattacacaa
     1261 aaagtgtcat ctggcgttac actttatgcg gatactaaaa caggaggttt tatgaacaga
     1321 acgattcttg tccctatcga tatttccgat tcagaattaa ctcaacgcgt gattagccac
     1381 gttgaggcag aggcaaagat tgatgatgca gaggttcatt tcctgacggt aataccttca
     1441 ctgccctact atgcctctct gggtttagcg tattccgcag aattaccggc aatggatgac
     1501 ctgaaagcgg aagccaaatc gcaactggaa gagatcatta aaaaatttaa actgccaacc
     1561 gacagagtgc atgtccatgt tgaggaaggt tcgcccaaag accgtattct ggaattggcg
     1621 aagaagatcc ccgctcatat gatcatcatt gcttcccatc gaccggatat caccacttat
     1681 ctgctcggtt ccaacgccgc agctgtagtg cgtcacgcag agtgctccgt gctggttgtg
     1741 cgctgacact aacgcccgca cattgctgcg ggctttttga ttcatttcgc aaatgtgctg
     1801 acattttccc ctctaatccg taccatacac gccacagttt ttatatcaga tttcttatgc
     1861 tgggcgttcc ggcatgacgc atactcttct gatgccatat aacgaattga gtcgctttta
     1921 aatgtcgcaa aatcaagaaa ttagcaagaa agaacaatac aatctgaaca aattacaaaa
     1981 acgcctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga
     2041 tcgcatcatg gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg
     2101 caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa
     2161 gcaaccgggc ttcccggaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta
     2221 caagattgtt gaagagaaca cttacggtat cgtgaaagag aagattccag agggcaaaac
     2281 cacttgctca ctgtgttctc gacttcgtcg cggtatcctt tatcgcaccg caacggaact
     2341 gggggcgacg aagatcgcgt tgggccacca tcgtgacgat atcctgcaaa cgttgttctt
     2401 aaatatgttc tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg
     2461 caaacatatc gttattcgtc cgctggccta ctgccgcgag aaagatattc agcgatttgc
     2521 cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca
     2581 acgtcaggtg attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga
     2641 gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt
     2701 cgatttcaaa ggcatcaccc acggttctga agtggttaac gggggtgatc tggcgtttga
     2761 tcgcgaagag atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt
     2821 ggatgagtta cggctgaatg tggttgaagt gaaataacca ggatagcgcc cgatgcgcaa
     2881 gcgtatcggg ctactcttat ggaggccgga taagacgcag ccagcgtcgc atccggcaat
     2941 cccgaataag atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg
     3001 cacccggcac gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc
     3061 aacagcctga cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc
     3121 tgcgccatca agcccgatat atcctgtcag tgcacctaat acatcacccg ggcgcatttt
     3181 ggcttttttc ccgccatcga tacacaacgt tgccatttct gcttccagcg tcacaatgga
     3241 actattagct ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt
     3301 ggcccgctgt gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg
     3361 agctgtacga ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac
     3421 caccagctca agcgatttaa tatccagacc acgcgcagca acatcagtcg cgaccagtac
     3481 acgggcgcta ccgttagcaa aacgtaccag ggtctgatcg cgatcgcgtt gctccaaatc
     3541 gccgtgtaat gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagcctggca
     3601 atcttttttg gtattgcaaa acaccacgca agaggatggc tgatgcaagc ttaataactg
     3661 ttgcaacaga ggaattttgc ctttactgga tgtctcataa aattgttgtt caatgggtgg
     3721 caaagcatct gttgagtcaa tttcaatcgc caaaggatcg cgttgcactc gtccgctgat
     3781 tgcggcgatg gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgccggcgc
     3841 aaaacggatg acatcatcaa tggcatcgct aaatcccata tccagcatgc ggtcggcctc
     3901 atccatcacc aaagtattca gcgcatccag tgataccgtg cctttttgca ggtgatccag
     3961 caaacggccc ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat
     4021 accgaacggt tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag
     4081 ccgacgcaat tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc
     4141 ctgggtttga aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt
     4201 tttgccgctg ccagttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag
     4261 cgcggcggcc tgcaccggcg tcatggttaa ataacccaac tcattaaggt tcgtgagttg
     4321 ggcgggaggc aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg
     4381 acgcaaagtt agcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc
     4441 ggttcttcat cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact
     4501 ggaacaggat cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc
     4561 atatttccct ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca
     4621 aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc
     4681 aaaaaaggaa gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg
     4741 caagagagat attgccctga atgggtagag agtttattga cttcgctcaa actttgcggc
     4801 gtttttgtat acagatagcc ggaaaaattg cttttgttac aaccatttac tacgatgcaa
     4861 ccataaagca acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccgaa
     4921 ttgccacccg ccgccaggga tcccaccaag gttgacgcca aataaccctg tcagaaaggt
     4981 actgggtaaa aagaccattg ccatcaatga cattgtatag gtacgacgag ctaaattttc
     5041 ctgcatcacc tgagcgattt catccgccat cacaccagtc cgtgctatac aggcgtcgat
     5101 ttcgtcaagg ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc
     5161 gctcatccac ggcaaacgtt cactggcaag acgagcatag acatcacgtt gcggtgccat
     5221 atagcgacgc atcacaatta attgtttgcg cagcagagcc aggaatccac gcggcggaat
     5281 ttgctgatca aggagattat cttcaaggtc gataatttta tcgtgcagct gctcgataaa
     5341 ttcgctggaa tgatcggtca acgcatcgca cacatccacc agccaccctc cgcaatcggt
     5401 cggacccgtg ccctcttcca gatcgctcac cacatcgtcc agcgccaaca ctttgcgttg
     5461 tcgggtcgaa acaattaacc gcccgtccat atatacacgc atggcgacca gttgatcggg
     5521 gcgttcatcg gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgagacg
     5581 gctgactcgg ggccgtgtgc tctcacctgc cagcgcatca cgtacgttat tgggaagcag
     5641 cggtgttgtc gccagccatt gggcgctatc atggtgtaca taattaaggt ggagccagca
     5701 gggatgcgct tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc
     5761 cagcatccag gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac
     5821 agtgcctcca tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca
     5881 tctcatcacc ccattaccct gaaatgatta ataaaattct gtctaaattg aatacaaaaa
     5941 gcaaaatgct tttccgtata caaaccgtgt gaagtgttaa atagcgtcta tcattatcag
     6001 aattatctga tcatatgacg tgcttttttg cgatcggata gcaacaaaaa ttgataaaaa
     6061 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
     6121 ctggatcgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt
     6181 gatgcaagac ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa
     6241 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
     6301 tgttcagcgt aaagaagagg aaacagcatt aagctgtcgg cttgttttgc gagagctgac
     6361 agaaacagaa ccggtgatta ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa
     6421 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct
     6481 gactaactct gcaccaatgt tgttgattga cccgtcacga gatggccaaa tcgtcgatgc
     6541 taacctcgcc gcgctcaatt tctatggtta taaccatgaa acgatgtgcc agaaacatac
     6601 ctgggaaata aatatgctcg ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt
     6661 acccggcggt cataaacctt tgaattttgt tcataaactg gcggatggat ctactcgtca
     6721 tgtgcagacc tatgccggac cgattgaaat ttatggcgac aagctcatgt tatgtattgt
     6781 gcatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg ctcaccatga
     6841 cgcgatgacc ggattactga atcggcgaca gttttatcac attacggaac caggccaaat
     6901 gcagcatctc gccatcgctc aggattacag cttgttgctc atcgacaccg atcgttttaa
     6961 acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg
     7021 caccctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt
     7081 tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg
     7141 cgtaagcgtg gcaaaagtga gtatttcggg cttaccacgc tttaccgtca gcattggtgt
     7201 ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt
     7261 gtatcgggcg aaaaatgatg gtcgcaaccg cgtgctggtg gcataagccg cggatgcgtc
     7321 tcgagatcaa cgactgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt
     7381 tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg cggtgcaata
     7441 tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc
     7501 atgcgattta tcatcccgcc ctttaccatg aataatcagc acgttacgca aaccatccgc
     7561 cagggcttgt tgaataaaac tgaacaccat tttgcggcac tcttccaccg gctggcgcaa
     7621 aagattcagg ctcgcctgtt gcggatattt accactacgc agcttatcca gcaccccatg
     7681 ttgcaaccct tcccgccgaa actccagcgg ctgacttagc gggataatgt cgagaaatcc
     7741 ggtagtgagg aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg
     7801 cgttggatgc cagtggacat cggtagcacg tttcagcggc tggacatctt ccatggcgtc
     7861 aagaaacagc gatttgtcgt caaggttcat gttacatcct cccgcaatta agagcgcgat
     7921 atgataacca gaccgggtcg gtccaacaac gtattaccca aaattccagt aataagttcc
     7981 aaatattgcc gatattttaa gcaaaatgct tatgcatgat tattcattca cgatattaat
     8041 aatgtaactt atattttcgt gaaatctgtc actgaagaaa attggcaact aaaggttaaa
     8101 accgttataa cacagtcacc ggcgcagagg agacaatgcc ggatttaaga cgcggatgca
     8161 ctgctgtgtg tactgtagag tctggcggat gtcgacagac tctatttttt tatgcagttt
     8221 taactttgca gatagccgca ttctcgccgg aattgcgtga ttaatgacgc ggtcagtggt
     8281 gtctggcgac tatcacgccg ctgaatcaaa taataggccg ctttcggtaa aatttctcta
     8341 accggcaaca tcaccagccc ctgtccgtgc aagggatcgc agcccatttc ttcaggcagt
     8401 atgctgagaa aatcgctttt tgccaccaga ctgatacagg ctgagaacgt ctcgcagact
     8461 acaccgacct gtggcgtttg cgcctgatcg tcaagcaatt cactcaactg tttgtagtag
     8521 ctgccgtgtg gcgtcggcat tgtccagctg taatccagta actgtttgat cgaacgggca
     8581 ccaatggcgg ggtgtcccgg gcggcaaaag atcgcgaatt gcttttccag taatttctca
     8641 aaagtaaatt cgtggtcgta cggtccctga taataggtat tgatggtgaa atccaattct
     8701 ccctgacgca attcattaat catcgacacc agttgccctt ccataatgcg tacttttacc
     8761 tgcggatgct gctgatgaaa acgagatatg acagctggca tcagactgcg ggaaatactg
     8821 gcccccatgc cgatattaat ctgccctgcc agttgccctt gtcgttggcg aatatcctct
     8881 tgggctgcgc gcagctcttc aagaattaga ctggcgtgct gataaaaact ttcaccggca
     8941 tcagttaacg tcacgccttt actacggcga aaaaagagtt gcgccgctaa cccttcttct
     9001 agctcctgaa tagatttact cagtgccggt tgcgacatat tcaacattcg gctcgctccg
     9061 cgaatgctgc cctgacgagc cacttcaaca aaagcccgaa tttgatgaat ttttacctga
     9121 aaagccatga cgccaccgat aaccgttatt tatcagacca aagaaactgg catctacttt
     9181 aatgcagatg attgtgtcag ggtaatttat gaacggttaa aactgtgaaa aatcagttag
     9241 tgataagtaa aaactatcgc tacgtgaacc gggtcacact ttttactgat gacgggaaag
     9301 gttatggagt ctttgaatca atttgttaat tcgcttgccc caaaattatc gcactggcga
     9361 cgtgattttc atcactatgc agagtctggc tgggtggaat tccgcactgc cacccttgtt
     9421 gcggaagaat tgcaccagct cggctattca ctggcgctgg gtcgcgaagt agttaatgaa
     9481 agtagccgga tgggattacc tgatgaattc actctacaac gcgaattcga gcgcgctcgt
     9541 caacagggtg cgctagcaca atggattgcg gcttttgaag gtggtttcac tggtatcgtc
     9601 gccaccctgg ataccggtcg ccccggtccg gtgatggctt tccgtgtcga tatggacgcg
     9661 ctggatctca gtgaagagca ggatgtcagc catcgcccct accgcgacgg ttttgcgtca
     9721 tgtaacgccg gaatgatgca tgcctgtggt catgatgggc ataccgccat tgggcttggg
     9781 ctggcgcata cccttaaaca gttcgagtcc ggactacatg gcgtcatcaa actgattttt
     9841 cagcctgcag aggaaggtac gcgtggcgcg cgggcgatgg tcgatgcagg tgtagtggat
     9901 gatgttgatt attttactgc cgtgcacatt ggcactggcg tacctgcggg caccgtggtg
     9961 tgcggcagtg ataattttat ggcaaccacc aaatttgacg cgcactttac cgggaccgcc
    10021 gctcacgcag gcgcaaaacc agaagacggt cacaatgcct tgttggcggc agcacaagca
    10081 actcttgcac tacatgcaat cgccccgcac agcgaaggcg cttccagagt aaacgtgggc
    10141 gttatgcagg caggaagcgg tcgtaatgtt gttcctgcct cggcgttgct gaaagtggaa
    10201 acacgcgggg ccagcgacgt cattaatcaa tatgtttttg accgtgctca gcaagcgatt
    10261 cagggcgcag caaccatgta tggtgtcggc gttgaaactc gtctgatggg tgcagctacc
    10321 gccagttctc cttcgccgca atgggtcgca tggttgcaaa gtcaggcggc tcaggtcgcg
    10381 ggggtcaatc aggccattga acgtgttgaa gcgcctgcgg gttccgaaga tgccacatta
    10441 atgatggccc gcgtgcagca acatcaaggg caagcctcct acatggtgtt tggcacacag
    10501 ctggcggcag gtcatcacaa cgaaaaattc gattttgacg agcaggttct cgctattgcc
    10561 gtcgaaacgc tggcgcgcac cgcgctcaat tttccctgga cgcgaggtat ctgatgcagg
    10621 aaatctatcg ttttatcgac gatgcgattg aagccgatcg ccaacgttat accgatattg
    10681 ccgatcaaat ctgggatcat ccagaaacac gttttgaaga gttctggtca gcggagcatc
    10741 tggcttcggc gctggaatct gcaggcttca ccgttacccg caacgtaggc aatatcccaa
    10801 atgcctttat tgcttcgttt ggtcaaggca aaccggttat cgccctgctg ggagaatatg
    10861 acgccctggc aggtttaagt cagcaagcag gttgcgcgca acctacatcc gtgacgcccg
    10921 gtgaaaatgg tcacggttgc ggacacaatt tgctgggaac cgccgccttt gccgctgcaa
    10981 tagccgtcaa gaaatggctg gaacaatatg ggcaaggcgg cacggtgcgc ttttatggtt
    11041 gtcctggcga agaaggcggc tcgggtaaaa cgttcatggt tcgcgagggg gtatttgatg
    11101 atgtggatgc ggcactcacc tggcacccgg aagcctttgc cggtatgttc aatacccgta
    11161 cgctggcaaa cattcaggca tcatggcgct ttaaagggat cgcagcacat gccgcgaatt
    11221 cccctcattt gggacgcagc gcccttgatg ccgtaacgtt gatgaccact ggcaccaact
    11281 tcctcaacga acatattatt gaaaaagcgc gcgtacacta tgccatcaca aatagtggcg
    11341 ggatctcgcc caacgtggtc caggcgcagg cagaagtgct ttatcttatc cgcgcccccg
    11401 aaatgaccga cgtgcagcat atttatgatc gagtcgccaa aatcgccgaa ggtgcggcat
    11461 tgatgaccga aaccacggtt gaatgccgct tcgacaaagc ctgttccagt tatctcccga
    11521 atcgcacctt agaaaatgcc atgtaccagg ccctatccca ttttggtacc ccggaatgga
    11581 actccgaaga actggctttt gcgaaacaaa ttcaggctac gctcaccccc aacgatcggc
    11641 aaaacagtct gaataatatc gccgcaaccg gtggcgaaaa cggcaaggct tttgcactac
    11701 gtcatcgtga aacggtactg gcgaatgaag tcgctccata tgccgccacc gataacgtgc
    11761 ttgcggcatc gactgatgtc ggcgacgtca gttggaaact gcctgttgcc cagtgtttca
    11821 gcccctgttt tgccgtcggt acaccgctac atacgtggca actggttagc caggggcgaa
    11881 catctattgc tcataaagga atgctgctgg cggcgaaaac tatggcagca accacactca
    11941 atctcttcat tgattcaggg ctattgcaag aatgccaaca agagcatcag caagttacgg
    12001 acacgcaacc gtatcactgc cctatctcga aaaacgtgac accgtcacct ttaaaataac
    12061 aacaacaacg caaacacaac aaccgaggaa tgcccatgag tatgtcatcc ataccgtcgt
    12121 cctcccaatc cgggaagctc tatggctggg tcgaaagaat tggtaacaag gttccccatc
    12181 cttttttgct ctttatctat ttgattatcg tactcatggt gacgacggca attttgtcgg
    12241 cctttggcgt cagtgcgaaa aacccgaccg atggtacgcc ggtggtggtg aaaaacctgc
    12301 tcagtgtgga aggattacac tggtttttac ccaatgttat taaaaacttt agcggttttg
    12361 ctccacttgg tgcgatcctg gcgctggttt taggtgccgg tctggcggag cgcgtcggct
    12421 tactgccagc gctaatggtt aaaatggcat cgcatgttaa tgcccgctac gccagttata
    12481 tggtgctgtt tattgctttt ttcagccaca tttcttccga tgcggcgtta gtgatcatgc
    12541 caccgatggg tgcgctgatt tttctggcgg tgggcaggca tccagttgca ggtttactgg
    12601 ctgccattgc aggcgtaggt tgcggcttta cggctaattt actgattgtc acaaccgacg
    12661 tgttgctgtc ggggatcagc acggaagcgg cggctgcgtt caatccgcaa atgcacgtca
    12721 gtgtaattga taactggtat tttatggcca gctccgtagt cgtactgacg attgttggcg
    12781 gcctgataac cgacaaaatc atcgagccac ggttaggtca atggcaggga aacagcgatg
    12841 agaaactgca gacattgacc gaaagtcagc gttttggttt acgcatagca ggtgtcgtat
    12901 cgctactttt tattgctgcg attgcgctga tggtgatccc ggaaaacggg atattgcgcg
    12961 atccgattaa tcacaccgtg atgccatcac cctttattaa aggtatcgtg ccactgatca
    13021 ttcttttttt ctttgttgtc tcgctggctt atggcatcgc tacccgcaca attcgacgtc
    13081 aggcggattt accgcattta atgattgaac cgatgaaaga gatggcggga tttatcgtga
    13141 tggtttttcc cctcgcccag tttgtcgcca tgtttaactg gagcaacatg gggaaattca
    13201 tcgccgtggg gctgaccgat atactggaaa gttcagggct tagcggcatc ccggcgtttg
    13261 tcggtctggc gttgctttcc tctttcttat gcatgtttat cgccagcggt tccgcaatct
    13321 ggtcgattct ggcccccatt ttcgtaccaa tgtttatgct acttggcttt cacccggcat
    13381 ttgcgcaaat cctctttcgt attgccgact catccgtatt gcctttagcg ccagtatctc
    13441 cttttgttcc actgtttctt ggattcctgc aacgctacaa accagacgcg aaactgggta
    13501 cttactattc gttagtcttg ccctatccgc ttatcttttt ggtggtatgg ctgctaatgt
    13561 tgctggcgtg gtatcttgtg ggcctgccga taggtccggg tatttaccca cgtttgtctt
    13621 aagagagaac ggatgctgag attacttgaa gaaaaaattg ccacgccact gggtccactg
    13681 tgggtgattt gcgatgagca atttcgcctg cgggcggttg aatgggaaga gtacagcgaa
    13741 cgcatggtgc agctgctgga catccattat cgcaaagaag gctatgagcg catttctgcc
    13801 accaatccag gcggtttaag cgacaagctt cgtgaatatt ttgccggtaa tcttagcatt
    13861 attgatacgc ttcccactgc tacggggggg acgccatttc agcgcgaagt ctggaaaaca
    13921 ctacgcacta tcccctgcgg gcaggtaatg cattacggcg aactggctga gcaattgggc
    13981 cgtcctggcg cggcgcgtgc cgttggtgcg gcaaacggat cgaatcccat cagcatcgtc
    14041 gtaccttgcc atcgggttat tggccgaaac ggcaccatga ccggatatgc aggcggagtt
    14101 cagcgaaaag agtggttatt gcgccacgaa ggttatcttt tgctgtaaac attaaacaat
    14161 ttgtgccagc ttgttcacac ttttatgtaa agttaccctt aacaacttaa gggttttcaa
    14221 atagatagac atatatttac atctaatatc ggaattctct gctgttaagg tttgcttaga
    14281 cttacttgct ccctaaaaag atgttaaaat tgacaaatat caattacggc ttgagcagac
    14341 ctatgatccc ggaaaagcga attatacggc gcattcagtc tggcggttgt gctatccatt
    14401 gccaggattg cagcatcagc cagctttgca tcccgttcac actcaacgaa catgagcttg
    14461 atcagcttga taatatcatt gagcggaaga agcctattca gaaaggccag acgctgttta
    14521 aggctggtga tgaacttaaa tcgctttatg ccatccgctc cggtacgatt aaaagttata
    14581 ccatcactga gcaaggcgac gagcaaatca ctggtttcca tttagcaggc gacctggtgg
    14641 gatttgacgc catcggcagc ggccatcacc cgagcttcgc gcaggcgctg gaaacctcga
    14701 tggtatgtga aatcccgttc gaaacgctgg acgatttgtc cggtaaaatg ccgaatctgc
    14761 gtcagcagat gatgcgtctg atgagcggtg aaatcaaagg cgatcaggac atgatcctgc
    14821 tgttgtcgaa gaaaaatgcc gaggaacgtc tggctgcatt catctacaac ctgtcccgtc
    14881 gttttgccca acgcggcttc tcccctcgtg aattccgcct gacgatgact cgtggcgata
    14941 tcggtaacta tctgggcctg acggtagaaa ccatcagccg tctgctgggt cgcttccaga
    15001 aaagcggtat gctggcagtc aaaggtaaat acatcaccat cgaaaataac gatgcgctgg
    15061 cccagcttgc tggtcatacg cgtaacgttg cctgattttt ccgcataact cactatcctt
    15121 ctgtcatatc attaaatttt tctgatttat tgatctggca gaaggttcat cactgtttca
    15181 ttcaccagat atgggttaat cttttaatta caaactgcgt tgacagttgt tgtaaggaga
    15241 ccctgtatgg ctatgtatca gaacatgctc gttgttatcg atcctaacca ggacgaccaa
    15301 ccagcattgc ggcgagctgt ttatttacat caacggattg gtggcaaaat taaagccttt
    15361 ttgccgatct atgacttctc atacgaaatg accaccctgc tctccccgga cgaacgtacc
    15421 gctatgcgtc agggcgtcat cagccagcgt acagcctgga tccacgagca ggcaaaatat
    15481 tatctcaatg ctggcgttcc cattgaaatt aaagtggtct ggcataaccg tcctttcgaa
    15541 gccatcattc aggaagtgat cagcggcggg cacgatttgg tgctaaaaat ggcgcaccaa
    15601 catgaccgtc tggaagcggt gatttttacg ccaacggact ggcatctgtt acgcaaatgc
    15661 ccaagcccgg tgtggatggt gaaagaccag ccgtggccgg aaggaggtaa ggcgctggtg
    15721 gcggtgaatc tcgccagtga agagccgtat cataatgcgc tcaatgaaaa actggtcaaa
    15781 gagacgatcg aactggcaga acaagtcaac cataccgaag ttcatctggt tggcgcttac
    15841 ccagtaacgc caattaatat cgcgattgaa ctgccggaat ttgatccgag cgtttataac
    15901 gatgccattc gtgggcaaca tttgctggca atgaaagctc tgcggcagaa attcggcatt
    15961 aatgaaaaca tgacgcacgt agaaaaaggt ctgccagaag aggtgattcc tgatttggcg
    16021 gagcacttac aggcggggat tgtggttctg ggcacggtag gacgcaccgg tatttcagca
    16081 gcattcctcg gcaacacggc ggaacaggtg attgatcatc ttcgctgcga tctgttggtc
    16141 attaaacctg accagtatca gacacccgtt gaactggatg acgaagaaga cgattaacga
    16201 ctcttcaata actgacaagg cccgagttat cgggcctatt ttttacttac tgctcttcgc
    16261 cgccaaggaa ataaattccc aacgggattg ccagcaacac agtaaagacc aggctataaa
    16321 caaaaatcat cgatgactga atcacataca ttgatgtcgt ccactcagac agcggaatat
    16381 tgtactgttc tataacgccg ccgatagtcg cgcgagtcac aaccgacgcc gcaataatga
    16441 aaaccgccaa caaaccaaag agaaatttct tccctttcgc tgacttcagt ttcgccataa
    16501 tcatgatgta atccttgtgc agacaaattt cttgttacct aaacgtaaca ataccatgac
    16561 atttcaccgt tgtctgctct gaaaacctca cctcttagct ggatttacaa tcaacgcatt
    16621 gattagttag ctaatgttgc tacttccttc cctgactatt gatgatctga atgaattgtc
    16681 gcggtaaaat agccccgcca ggggtttccg gcatgacgac aattatctgg aggagcctga
    16741 taaaacaatg atcgctgaac tgtttacaaa taatgcgctt aatctggtca ttattttcgg
    16801 tagctgcgca gcattgattc tgatgagctt ttggtttcgc cgcggaaatc gtaaaagaaa
    16861 aggcttttta ttccatgcgg tgcaattttt aatctacacc ataattatca gtgctgttgg
    16921 tagcattatt aattatgtta tagaaa
//
LOCUS       DAZGBC010000104        16859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGBC010000104 DAZGBC010000000
VERSION     DAZGBC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.105"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..490
                     /locus_tag="ACHK1J_003311"
     CDS             <1..490
                     /locus_tag="ACHK1J_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFO7912546.1"
                     /translation="LLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMI
                     IAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVIS
                     GITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLP
                     FL"
     gene            complement(546..3401)
                     /gene="valS"
                     /locus_tag="ACHK1J_003312"
     CDS             complement(546..3401)
                     /gene="valS"
                     /locus_tag="ACHK1J_003312"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO7912547.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEYN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3401..3844)
                     /gene="holC"
                     /locus_tag="ACHK1J_003313"
     CDS             complement(3401..3844)
                     /gene="holC"
                     /locus_tag="ACHK1J_003313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO7912548.1"
                     /translation="MKNATFYLLDNDITVDGLNAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(4198..5709)
                     /gene="pepA"
                     /locus_tag="ACHK1J_003314"
     CDS             complement(4198..5709)
                     /gene="pepA"
                     /locus_tag="ACHK1J_003314"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO7912549.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5863..5913
                     /gene="ytgA"
                     /locus_tag="ACHK1J_003315"
     CDS             5863..5913
                     /gene="ytgA"
                     /locus_tag="ACHK1J_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO7912550.1"
                     /translation="MSIIILATISTTQENK"
     gene            5976..7076
                     /gene="lptF"
                     /locus_tag="ACHK1J_003316"
     CDS             5976..7076
                     /gene="lptF"
                     /locus_tag="ACHK1J_003316"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7912551.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7076..8158
                     /gene="lptG"
                     /locus_tag="ACHK1J_003317"
     CDS             7076..8158
                     /gene="lptG"
                     /locus_tag="ACHK1J_003317"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7912552.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8319..9821)
                     /gene="yjgR"
                     /locus_tag="ACHK1J_003318"
     CDS             complement(8319..9821)
                     /gene="yjgR"
                     /locus_tag="ACHK1J_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO7912553.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASNEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(9899..10897)
                     /gene="idnR"
                     /locus_tag="ACHK1J_003319"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(9899..10897)
                     /gene="idnR"
                     /locus_tag="ACHK1J_003319"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO7912554.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRTAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(10964..12283)
                     /gene="idnT"
                     /locus_tag="ACHK1J_003320"
     CDS             complement(10964..12283)
                     /gene="idnT"
                     /locus_tag="ACHK1J_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO7912555.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12346..13110)
                     /gene="idnO"
                     /locus_tag="ACHK1J_003321"
     CDS             complement(12346..13110)
                     /gene="idnO"
                     /locus_tag="ACHK1J_003321"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO7912556.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(13134..14165)
                     /gene="idnD"
                     /locus_tag="ACHK1J_003322"
     CDS             complement(13134..14165)
                     /gene="idnD"
                     /locus_tag="ACHK1J_003322"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO7912557.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14382..14945
                     /gene="idnK"
                     /locus_tag="ACHK1J_003323"
     CDS             14382..14945
                     /gene="idnK"
                     /locus_tag="ACHK1J_003323"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO7912558.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPHADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(14949..15969)
                     /gene="ahr"
                     /locus_tag="ACHK1J_003324"
                     /pseudo
     CDS             complement(14949..15969)
                     /gene="ahr"
                     /locus_tag="ACHK1J_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
     gene            16165..16249
                     /locus_tag="ACHK1J_003325"
     tRNA            16165..16249
                     /locus_tag="ACHK1J_003325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16199..16201,aa:Leu,seq:caa)
BASE COUNT         4000 a         4421 c         4206 g         4232 t
ORIGIN      
        1 tttattacct tttctcgctg ttgctggtta tattatcttc gatcaaatat taaatgcgta
       61 tgatagttct gtgtatgcaa atgatgatat tgagaattta cagcaattta tggaaatgca
      121 acgtaaaatg ataatcgcgc agttaatcta ttattttggg attgctgtta gcaccagtta
      181 tttaacggtg tcgttgcgaa atcattttat gagcaacctg tcactgaatg atgggcgtat
      241 tcgttttcgc tcaactttaa cataccacgg tatgctttat cgcatgtgtg cgttggtggt
      301 gatatccggg attacgggcg gtctggctta tccactgctg aaaatatgga tgattgactg
      361 gcaggcaaaa aatacgtatt tgctgggcga tttggatgac cttcctttaa tcaataaaga
      421 agaacaacca gataaaggct tcttagccag tatttcacgg ggaattatgc cttctttacc
      481 atttctgtaa gagataaaaa aggccggagc atgctccggc cttcgttttc atcactgtgt
      541 tttgattaca gcgcggcgat aacagcctgc tgttcaatca gtttcgcttt cgcttctgca
      601 tagccttcca gcttctcacg ctctttcgcg atgaccgctt ccggtgcgcg ggcgacaaag
      661 ccttcattcg ccagcttgtt ctcgatacgg ctgatttcgc cttcgatctt cgccacttct
      721 ttcgccagac gcgccagctc atcttctttg ttgatgaggc cagccatcgg gatcagcagc
      781 tctgcgccgt cgatgatctt cgtaacggaa accggacctt tgtcatcggc aggcagcacg
      841 gtgatgcttt ccagacgcgc cagcgtttgc aggaagccac ggttttcatt tacgcgacgt
      901 tctgcatccg cgctgcaacc acgcagcagc agctccagcg gtttgcccgg cgcgatattc
      961 atttctgcac ggatgttacg tacggcaacg atcgcctgtt tcagccactc ggtgtcggcc
     1021 agcgccgctt catcaacctg agacgcatca tactgcggga acggctgcag catgatggtg
     1081 tcggcagtga taccgcaaag tactttcaca cgctgccaga tggtttcggt gatgaacgga
     1141 atgatcggat gcgcgaggcg cagcagacct tccagtacag tcaccagcgt atggcgagta
     1201 ccgcgcagtt cggcttcggt gccaccgttc attaccggct tggtcagctc cagataccag
     1261 tcgcagaact ggttccaggt gaactcatac agaatgcctg cagcgatatc gaagcggaag
     1321 ctgtccagcg cttcgcggta cgctttgatg gtctggttgt actctgccag aatccagcgg
     1381 tccgccagcg acagcgtcat ttcaccaccg ttgaagccgc aatcctgacc ttctgtgttc
     1441 atcagcacaa agcggctggc gttccacagc ttgttacaga agttacggta accttccagg
     1501 cgcttcatat cccagttgat gtcacgaccg gtagaagcca gcgccgccag ggtgaagcgc
     1561 agggcatcgg tgccgtgcgg ctcaatgccg ttcgggaact gtttctcggt gcgcttacgg
     1621 attttgtccg ccagctgcgg ctgcatcata ttgccggtac gtttttccag cagttctggc
     1681 agcgaaatac cgtcaaccat atccagcgga tcaataacgt tacccttgga tttagacatc
     1741 ttctggcctt cgtcatcacg aatcaggccg gtcatgtaaa cggtgtggaa cggcacctgc
     1801 ggtttgccat tttcatcttt gatgaagtgc atggtcatca tgatcatgcg ggcaatccag
     1861 aagaagatga tgtcgaagcc ggataccatc acgctggttg ggtggaactg acgcagggcg
     1921 tcggtgtttt ccggccagcc aagggtagag aaggtccaca gtgcagaaga gaaccaggtg
     1981 tcgagaacgt cttcgtcctg acgcagggca acgttggcac cgaggttatt ttccttgcgc
     2041 acttcttctt cgttgcggcc aacataaaca ttacccgctt cgtcatacca tgccgggata
     2101 cggtgacccc accacagctg acgagagata caccagtcct gaatatcgcg catccaggag
     2161 aagtacatgt tttcgtactg cttcggtacg aactgaatgt cgccgttctc aaccgcttca
     2221 accgccggtt tcgccagtac atcggcacgc acgtaccact ggtcggtcag cattggttcg
     2281 ataactacgc cgccacggtc gccgtaagga acggtcaggt cgtgcggttt aatttcttcc
     2341 agcaggccga gtgcgtcaac cgcagcaacg accgctttac gtgcagcaaa acgctcgagt
     2401 ttctggaact ctgccgggat ttcgctggaa taaacgtcag attcgttacc tttggtatcg
     2461 aacacctggg cgctttcacg gatatcgccg tcaaaggtca ggatgttgat catcggcagc
     2521 gcgtgtcgtt tacccacttc atagtcgtta aagtcgtgcg ccggagtgat tttcacgcag
     2581 ccggtgcctt tttccatgtc ggcgtgttcg tcgccaacga tcggaatacg acggttaacc
     2641 agcggtagaa tgacatattt gccaatcaga tctttgtaac gcggatcttc cgggttaacg
     2701 gctacgccag tatcgcccag cagggtttcc ggacgggtag tcgcgaccac cagataatct
     2761 ttaccgtctg cggttttcgc accgtcagcc agcggatagc ggatgtgcca catcgaacct
     2821 ttcgattcgc ggttttccac ttccaggtca gagatagcgg tgcgcagttt cgggtcccag
     2881 tttaccaggc gtttgccacg gtaaatcagg tcttctttat acagacgaac gaaaacttct
     2941 ttcaccgcat tggacaggcc ttcgtccatc gtgaagcgtt cacgctccca gtcgacggag
     3001 ttgccgagac ggcgcatctg acgggtaatc gtgccgccgg attccgcttt ccattcccag
     3061 attttgtcga tgaaagcttc gcggccgtag tcgtgacggg ttttaccttc ttctgcggca
     3121 atcttgcgct caacgaccat ctgggtagcg atcccggcgt ggtcagtacc gacctgccac
     3181 agggtgtttt tgccctgcat gcgctgatag cggatcatgg tatccatgat ggtttgctgg
     3241 aaggcgtgac ccatatgcaa actgccggtg acgttcggcg gcgggatcat gatgcagaaa
     3301 ctttcctggc tttcatcgcc attaggctta aagtagccct gcttttccca gtgctcgtaa
     3361 agcggctgtt cgatatcttg tgggttatat gtcttttcca ttatttccag gttgccgtat
     3421 tcaggttgaa accagccacg cggtaggctt tatagcgttc gcgcgccagt tgtttcagag
     3481 aatcttcata aggaacgaag tctaccactt ctgtgaaagc ggtggcaaaa tctgcaaagc
     3541 ttgttcgcag actaatcaat atatcgcgcg ggctgctgct gcgcttttgc ggccaggcga
     3601 tctccaccgg cgcaccgccg cgcggtcctt ctcccgctaa attatgcgga acaaagcttt
     3661 ctgccggacg cgcccacagg gcttcatcca gtcggtaagc ctgcttttca tcttcacagg
     3721 cgatgagtac gcgcttacca ctgcgccaac gttctgcggc aatttcacac accagttgct
     3781 caacggcgtt taagccatcg acggtgatgt cattgtccag aaggtagaac gtcgcgtttt
     3841 tcatatatgg ggcttcttgt ggtggattta aatacgcatc aggtatacac cgcagcgcct
     3901 gatgcgacgc tgttgtgtct tatcaggcct acgagttcag tgctgtgtgt aggtcggata
     3961 aggcgctcac gccgcatccg acaataacag cattgcctga tgcgacgctg ccgcgtctta
     4021 tcaggcctac gagttcggtg ctgtgtaggt cggataaggc gttcatgccg catccgacaa
     4081 taacagcatt gcctgatgcg acgcttgcgc gtcttatcag gcctacgagt tcagtgctgt
     4141 gtaggtcgga taaggcgttc acgccgcatc cggcaataac agccttgcct gacgcaatta
     4201 ctcttcgccg ttaaacccag cgcggtttaa caggaactgt gccagcaacg ctaccggacg
     4261 accggtggcg ccttttgctt taccagaacg ccaggcggta ccggcgatat ccaggtgcgc
     4321 ccagttgtac ttacgggtaa agcgcgacag gaagcaacct gcggtaatcg ccccaccagg
     4381 acgaccgcca atgttcgcca tatcggcaaa attggactcc agttgttcct gatactcgtc
     4441 acccagcggt aagcgccatg cgcggtcacc ggattgttca gacgcggcaa tcagttcatg
     4501 ggccagcgga ttatggttcg ccatcagacc agtaatatga tgacccagcg cgatcacgca
     4561 ggcaccggtc agcgtcgcca cgtcaatcac cgcttccggc tcaaaacgct caacgtaagt
     4621 taacacgtcg cacagtacca ggcggccttc agcgtcagta ttcagcactt caacggtttg
     4681 accggacatg gtggttaaca catcgcccgg acgataggct cgtccgccag gcatgttttc
     4741 gcagcctgcc aacacgccga taacgttaat cggcagttgt agctccgcga ccatccgcat
     4801 cacgccgtaa accgctgccg caccgcacat atcgtacttc atctcatcca tgccttctga
     4861 aggcttgatc gagataccgc cggagtcgaa ggttaaacct ttacccacca gcacgattgg
     4921 gcgtgcatct tccgacgcgt tgcctttgta ctcaatcacc gacatcagcg attcgttttg
     4981 cgaaccctga ccgaccgcca gataggaatg catccccagc tctttcatct gctgttcgcc
     5041 gataacgcgg gtgataacat tcttgctgta gctgtcagcc agctggcgcg cttgtgaagc
     5101 gaggtaagcg gcgttacaga tattcggcgg catattgcca agatcctttg ctgctttaat
     5161 cccggcggca atcgccagac cgtgctggat cgcgcgctca ccgctggtca gttcacggcg
     5221 ggtcggcacg ttgaacacca ttttacgcag cggacgacgc ggttcgctct tgttcgtttt
     5281 cagctgatcg aaactgtaga gcgtctcttt tgccgtctcg acagcctgac gcactttcca
     5341 gtagttgtta cggcctttaa cgtgcagttc agtcagaaag cagaccgctt ccattgagcc
     5401 agtatcattc agcgtattaa tggttttctg aataacctgc ttgtactgac gctcatccag
     5461 ctcacgttct ttgccgcaac caataaggag aattcgctcg gaaagtacat tcggaacatg
     5521 gtgcagcaac aatgtctgcc ccggttttcc ttccagttcg ccccgacgta gcagggcgct
     5581 gatgtaccca tcgctgattt tatcgagctg ttctgcaatc ggagaaaggc gacgtggttc
     5641 gaagacgccc acgacgatgc aggcactccg ctgtttctcc gggctaccgc tttttacact
     5701 aaactccatg cactacgctc ctgaatctta aagacaacgg cggtggctac agatagaatt
     5761 gcaagctttc gtaactcatg tccgctgttg cgatgacttc gtgttaatct taacgttatt
     5821 acggcattgg cacgtcagaa caaaatctga gaggtgaatc cgttgagtat aattatctta
     5881 gcgacgattt cgacgactca agagaataaa tgacgtttaa gccatgaaac aagctaaaat
     5941 cctgcaaaag acgagttttt acgggcgtat ttaaagtgat aatcataaga tatctggtgc
     6001 gggagacgct caaaagccag ctggcgatac tcttcatctt gcttttgatc ttcttctgtc
     6061 aaaagttagt gaggatcctc ggcgcagcgg ttgacggcga tattccggcg aatctggtgc
     6121 tctcccttct cgggttgggc gtgccggaaa tggcgcagct tatcctgcca ttaagcctgt
     6181 tcctcgggct gctgatgacg ctgggcaaac tgtataccga aagtgaaatt acggtaatgc
     6241 atgcctgcgg cctgagcaaa gcggttctgg tgaaagcggc aatgatcctt gcggtattca
     6301 cggcaatcgt cgcggcggtt aacgtgatgt gggcgggacc gtggtcatcg cgtcatcagg
     6361 atgaagtgtt agcagaagcg aaagcgaacc ctggcatggc ggcgctggcg caagggcaat
     6421 tccagcaagc gactaatggc agctcggtgc tgttcatcga aagcgttgac ggcagcgatt
     6481 tcaaagatgt gttcctcgca caaattcgac caaaaggtaa tgcacgtcct tctgtggtgg
     6541 tggccgattc cggacattta acccagctgc gcgacggctc ccaggtcgtc actctcaacc
     6601 agggaacgcg cttcgaaggc actgcactgt tacgtgattt ccgcattacg gatttccagg
     6661 attatcaggc gatcattggt caccaggcgg tggcgctcga cccgaacgat accgaccaga
     6721 tggacatgcg cacattgtgg aacactgaca ccgatcgtgc tcgcgcagaa ctgaactggc
     6781 gtatcacgtt ggtattcacc gtgtttatga tggcacttat ggtcgtaccg ctgagcgtgg
     6841 ttaacccacg tcagggacgc gtactgtcga tgctgccagc catgctgctg tatctacttt
     6901 tcttcctgat ccagacctcc ctgaaatcga acggcggtaa aggtaagctg gacccgacgc
     6961 tgtggatgtg gaccgttaac ctgatttatc tggctttggc gattgttctc aacctttggg
     7021 acaccgtgcc ggtccgccgc ctgcgcgcca gtttttcgcg taaaggagcg gtgtgatgca
     7081 accttttggc gtacttgacc gctatatcgg taaaactatt ttcaccacca tcatgatgac
     7141 gctgttcatg ctggtgtcgc tgtcgggcat tatcaagttt gtcgatcagc tgaaaaaagc
     7201 cgggcagggg agttacgacg cgttaggcgc aggaatgtat accttgctga gcgtgccgaa
     7261 agatgtgcag atcttcttcc cgatggcggc tctgcttggg gcgttgcttg gtcttgggat
     7321 gctggcgcag cgcagcgaac tggtggtgat gcaggcttct ggttttaccc gtatgcaggt
     7381 ggcgctgtcg gtgatgaaaa ccgccattcc gctggtctta ctgacgatgg cgattggcga
     7441 atgggtcgcg ccgcagggcg agcagatggc gcgtaactac cgtgcgcagg cgatgtacgg
     7501 cggctcgttg ctctctaccc agcaagggtt atgggcgaaa gatggcaaca acttcgtcta
     7561 cattgagcgg gttaaaggtg acgaagagtt aggtggcatc agcatttatg cctttaacga
     7621 gaatcgtcgt ctgcaatccg tacgctatgc cgctactgcg aagtttgacc cggaacataa
     7681 agtctggcgt ctgtcgcagg ttgatgaatc tgatctgacc aatccgaaac agattaccgg
     7741 ttcgcagacg gtgagcggca cctggaaaac caacctcaca ccggacaaac tgggcgtggt
     7801 ggcgctggac ccggatgcac tctccatcag tggtttgcac aactacgtga agtatctgaa
     7861 gtcgagcggt caggatgccg gacgttatca gctcaacatg tggagcaaaa tcttccagcc
     7921 gctatccgtg gcggtgatga tgctgatggc gctgtcgttc atctttggcc cactgcgtag
     7981 cgtaccgatg ggcgtgcgtg tggtcaccgg tatcagcttc ggttttgtct tctacgtact
     8041 ggaccagatc ttcggtccgc tgacgttggt ttacggcatc ccgccgatca ttggcgcact
     8101 gttgccaagc gccagcttct tcttaatcag cctgtggctg ttaatgagaa aatcgtaatc
     8161 taatgaacgc ataaagggag cgaaagctcc ctttattatt attagcaaag tgtgcttcgt
     8221 tcattcctga aaaattatga aaatccaata tattgcagtg gtgtaggcat gataagacgc
     8281 gccagcgtcg catcaggcaa agacaaatcg cacctggctt acctttttct cccccccagc
     8341 aaactcccca acatcccgcg tacaatctga ttcgtcacct ggcgagcggc gcttttggcc
     8401 attgtttgca ccacaccatc tttcttcccg ccgcgtggtc cggtagtgcc gaacaaaata
     8461 tccttcaatc caccaagaat gccgtcatct accgctacct ctttcccttt cgcgggggga
     8521 ttattttgct gctcgttact ggcctgaaag cctttttgca acatctcata ggcggattcc
     8581 cggtccacgt catcctcata tttgccatac accggggagt ggttaatcaa gccattgcgc
     8641 tcatcttccg tcaccggtcc catccgcgaa caaggcgcaa tcaccatcgc ccgttccacc
     8701 acagaaggac ttccttttgc atcgagaaac gagattaacg cctcgccggt ccccagttcc
     8761 tggattgcct tttcggtatc aaatgccgga ttggcccgca tggtttgcgc cgctgccttc
     8821 actgctttct gatcttttgg cgtaaaagcc cgcaaagcgt gctgaacgcg attaccgagc
     8881 tggccgagca cattatccgg aatatcagac gggttttgcg aaacgaacca gacgcccacg
     8941 ccttttgagc gaataagcct tatcacctgc tcaatcttat ccagcagtac ctgcggggca
     9001 tcgttaaaca gcagatgtgc ctcgtcgaag aaaaacacca gttttggttt ctccagatcg
     9061 cccgcttccg gcaattgttc atacaactct gaaagcatcc acagcaggct ggcggcgtac
     9121 agtttcggca tctgataaag tttctcggcg ctgaggatat tgataacgcc tttaccgttg
     9181 gcatcggtgc gcatccagtc tttgatatcc agcatcggct caccaaagaa gtgtgctgcg
     9241 ccttgctgtt ccagcgacaa cagcccgcgc tggatggcac caaccgatgc gctactgata
     9301 tttccgtact gattctggaa ggatttggcg ttatcgccga tgtactgggt aattgcccgc
     9361 agatctttaa agtcgagcag taacagcccc tgatcgtcag caatgcggaa gatgatattc
     9421 agcacgccag attgcacatc gttgagattc aacagccgcg ccagcaacag cggccccaga
     9481 tccgaaaccg tcgcccgcac cggatggcct ttctcgccaa agatatccca caccaccacc
     9541 ggattggcat gcggttgcca atcattgacg ccgatatttt taagccttgc gagcagtttt
     9601 tccgacgccg ttcctgcctg cgcgataccg gtcagatcgc ctttcacatc agccataaac
     9661 accggcacgc cgatttccga caatgactct gccagttttt gcagcgtaac ggttttaccc
     9721 gtccccgttg cgccagtaat cagcccgtga cggttagcca ttcccggcag taaaaatagt
     9781 tctgtgtccg gcgtgcgggc aattaacagg ggttcgctca tgagatttcc tccagtttcc
     9841 tgcctggagt ataggcaacg tccggcaaag agaatacgtg taatgaagag aaaataacct
     9901 aaagcgtgtt gccgtgataa atctgatacc cgaggtcaac agtgttgtgg ttgtgatcgt
     9961 tatttttaat tttgctcagc aacatttggg cagccgtccg accaatatca aaacggggag
    10021 taattacgct ggcgaggctt gggatcattt gcctgccaat ctccagccca tgaaaacccg
    10081 caatggaaat ttgttctggc acggcgaggt ttcgctcgcg gcacagcaga agcgcaccca
    10141 tcgcaatatc gtcattggtg caaaatacac cgtctaaatc cgggtttgcg ctaagggcat
    10201 cacgcattaa ctgcattcct aaatgaatgg atgagatggc gcgtggattc atgcgtaatg
    10261 gggaaagatt atgcagcatc atcgcatcgc aatacccctg ataacgctgt tcatcgcggg
    10321 tgtcatcttt ggaaccgaga tacagaattt tgtgcctgac tcgcttctcc agcatggtac
    10381 acaccatgtc aaaagcggcc tgccgattat caaaaccgac ctccatatcc agccgttctc
    10441 cctgtacatc catcaattcc acgaccggaa tggtggctga acgcagaaat ttcaccgtcc
    10501 tgatggtgtg atatttttcc gaaagtataa tcccgtcaat gttataagag agcaggttaa
    10561 tcaccgactc ttcttcggaa tcgcgatcgt aattgtaatt agcaataagg gtctgataat
    10621 tatgctcaga ggtgacggat tctattcccg cgagaatgtc agcgaagagt tgattttgaa
    10681 atgacggaat caagataccg agggtataac tttgtgcatt caacaacatg ccgggagcgc
    10741 gattagggat ataattgatc tcttccataa ttttggcgat gcgctcgcct gtttcctttg
    10801 ccaccttttt cggcgagcgg atataacgac tcacggtcat ttttgttacg ccagccagcg
    10861 tagcgatatc ctgtaaagaa attctgtgat tgcgcataac ttctcccaac gtcgggggaa
    10921 tcttcaatct tcccccgaaa ctacatcaca atattttatt ctttcagtgc aatacggcgt
    10981 taatggctaa cacgcccagc aaacccataa tagaaataag ggtttccatc accgtccagg
    11041 tacgcaacgt ttcaccaacc gtcagattaa aatacccttt aaatagccag aagccagggt
    11101 cgtttacgtg tgacgcgatc acactgcccg caccggttgc cagtaccatt aaagcgggat
    11161 cggcatgggt aacgttgatt atcggcaaca ccacacccgc ggtggtaatg gccgcgacgg
    11221 tggcagagcc cagagcgata cgcaacagcg ccgcaacagt ccagcacatc aataacggcg
    11281 aaagcgtagt tccggtcatt aagtgtgaaa tatactgccc gacaccgcta tctactaata
    11341 cctgcttaaa cgcgccgccg ccagcgataa taaacacaat catcgcgata gcgcctatag
    11401 aatccccaat gatatccatg atttgctcga tggtgcgtcc attacgtcgg cccagtgtga
    11461 aaatcgcaat aacaatggca ataaacagcg caacggcagg gttaccgaca aattcaaaga
    11521 agaggcgcac ggtgttagtt ttcggtaacg taatttcaca aacggcggcg atagccatca
    11581 ggatgaccgg gatcacggca gcgaaaatac tgttccagaa ggagggcatc tcctcttcgc
    11641 taaacagatg aggattaaat aagccttccg gtggtgcttt ctcaaagcga gttagcagtt
    11701 tagaaaacag cggtcctgcg acaataactg tcggaatggt aatgataaat ccatacagta
    11761 aagtcgttcc gagattagcc tcaaagatag tcgcgatggc agtaggccct ggatgtggcg
    11821 gcagaaaaca gtgggttaca gagagcgctg ctaccattgg tacgccaaca tacaacaggg
    11881 gtaatcctga tgatgctacg atggtaaata ccaacggcaa cagcaggaca aaacccactt
    11941 caaaaaacat ggcgaggccc acaaccagac cggtgatcac tagcgcccat tgcacgcgtt
    12001 ttttaccaaa agtagcaatc agcgtagtgg cgatacgttg tgccgcaccc gtatcagaaa
    12061 tcagcttgcc taacatggcc ccgaaaccga ggatcattgc cagcccgccg agcgtgctgc
    12121 cgataccatt ttgtatagag tgcaggacgg cctgtgcatc catcccttcg gcaaatccga
    12181 cgacggcagc taccagaacg agggcaataa agccgttaac tttaaagccg atcatcagga
    12241 taagaagcag cgcgacgcct gccgcaataa tgattaatgg catagtcttt atctcttatt
    12301 agtgccagaa aagcagcgct ttcgcgctgc acgtaggggg ggctgttaaa cagccactaa
    12361 catgccgcca tcaacaaaca acaggtggcc gtttacgaaa tcagaggctt ttgaagaaag
    12421 gaacaccgca gcaccaatca gttcctgcgg atctccccag cgtgcggcgg gggtccgttt
    12481 gcacaaccag gcggtgaagg cttcgtcctc aaccagtgct ttagtcattt ctgttttgaa
    12541 atagcccggc gcaataccgt tgacctgaat attgtggcgc gccagctcga cgcacatgcc
    12601 gcgggtgagc atttttaccg cccctttcga tgcggcataa ggggtgatgg tgtcacgtcc
    12661 cagttcgctt tgcatcgagc aaatattaat aactttacct gccttgcgtt caaccatgtg
    12721 acgagttacc gcttgcgata ccaggaacac ggcggtctgg tttactgcga tcacatcatt
    12781 ccactcttgt tcagggaact cagtaaaagg atgacggcgc tggataccgg cgttattcac
    12841 cagcacatca atggggccga tgtccttttc gatatgttca acggcggcat caatttcatg
    12901 tttatgagta acattaaaag gtgcggcaac ggcctgaata ccctcctggt ggagtttttc
    12961 tacagcaagt tctgcgcgtt cggcagtaat atcattaata attatttgtg cgccatattt
    13021 acccaggccg gttgccagta aaaagccaat gccctgtgct gaaccggtaa tcaagatatt
    13081 ttttcctgcc agtgaaaata gatcgttcat tgtattattc ctttatttat ctcttagaaa
    13141 acaagctgga cttttgctgc ctgggtttta tcaccggcga aacgtagcgc ctcttccagg
    13201 tcagtgaagg gatattcagc actcagtaaa ggcagtggat tgataacgcc attcgccagc
    13261 catgacactg cggtattaaa ttcgctggta aaacggaaag agcctctgag tgaaatctcc
    13321 ttaccaatca acgtcatcat tgggaattct gccatcgcgc ctcccatacc tacctgcacc
    13381 attacgccgc gtgcacgagt gacctccaga caggtattca ctgatgaagg atgaccggac
    13441 acttcaaagc tgacatcgaa atagcctttt tccgctttcc agtgatccat gtcgtcgttt
    13501 tgtgggttta cgagcacatc cgcccccatc tctttgccca gcgaaaggga acggggactc
    13561 acatcagcac agacaatttc cgcggccccc agtgttttca ctgcactgac aatcaggcag
    13621 ccaatgggtc caacaccgga aataaatact cgcttgccct gtaactcgcc ggcctgatgt
    13681 gcggcatgaa tcgcgacggc taaaggttcg gcaaaagcca taaccttttc gtcagctttg
    13741 gccggataag ggacacattg cgacgtttcg accattttat aacgggtaaa accaccatca
    13801 acatgaggga aatacatggc actgccaaaa aaacgcatat ctgtacactg attctcgtta
    13861 tgttcaatgc agtatttgca gtgaccgcac ggtttagacg gattaatggc taccgtttgc
    13921 ccttcatgta attctgatga gtcgctatga ataactttac cgataacttc atgacctaac
    13981 accatcggtg cctttatcat gaaattacct acttttcctt cctgataata atgtaaatcg
    14041 gaaccgcaaa ttccacctcg ggttatttgt actaatgttc cattattatt ccaatctatc
    14101 gtctgctcgg taacggcaac agttttcttg cccgcaacaa cgcaggactg tgttttcact
    14161 tgcataaatt cctcactggt caggtagaca cctcggaagc atttaagcgg ttttaattgt
    14221 cattatttgt gatgaagatc acgtcagaaa attgttacat tactatgtta cgcataacgt
    14281 gatgtgcctt gtaattctta tcagcagaaa taaaaaaacg tgaaattatt atgccgccag
    14341 gcgtagtatc gcagcaggta agatgattca ggagatttta aatggcgggt gaaagcttta
    14401 ttttgatggg cgtttcaggg agtggtaaaa cattaattgg tagcaaggtt gccgcgttat
    14461 tatctgctaa atttattgat ggtgacgatc ttcatccagc caaaaatata gataaaatgt
    14521 cgcagggtat tccattatct gatgaagatc gacttccctg gctggaacgc ttaaatgatg
    14581 cttcatacag tctttataaa aagaatgaaa caggatttat tgtctgttcg tcattaaaaa
    14641 aacagtatcg tgatatttta cgcaagggta gcccccatgt tcatttcctc tggttagatg
    14701 gcgactatga aactattctc gcgcgaatgc agcgtcgggc tgggcatttt atgccggtag
    14761 cgttactaaa aagtcagttt gaggcactgg agcgtccaca tgcagatgaa caggatattg
    14821 tgcgcattga tatcaaccat gatattgcca atgtcaccga acaatgtcgg caggctgtgc
    14881 tggcgatacg acaaaacaga atatgtgcga aagagggcag cgcctcagat cagcgctgcg
    14941 aatgattttc aaaaatcggc tttcaacacc acgcggtaac gcgccttacc gtcgcgcaca
    15001 tgctggatgg cgtcgttaat tttcgacatc gggaacagtt cggtagtcgg cgcaaccttg
    15061 ctgcgggcgg caaagcgcat cagcttacgc aactcataag gtgtaccggt agcagagccc
    15121 agagacactg cgatcgcccg caatcagcgt aaaggccgga accggcaacg gcgtgagaac
    15181 cgcaccgact gtatggaaat taccgccata ggtcagcgcc tcaaaatagg gctgccagtc
    15241 gaggctgacg ttgacggtgt tgataatgag atcaaactgc cccgccagtg ctttcagtgc
    15301 ctgcggatcg cggctattca ccactttatc ggcacccatc gccagcactt cctgctcttt
    15361 cgccggatta gaactaaagg ctgtcacctc gcatcccatt gcgtgcagaa gttttatagc
    15421 gatatgcccc agcccgccaa taccaattac cccaacgcgg ctggtagcag tgatatggtg
    15481 catcaacagt ggtttaaaga ccgtgatacc gccgcacaac agcggcccgg cggactcgat
    15541 atcaatattt tctggcagtg gaatcaccca ttgccagtcc gcacgcaact tctcggcaaa
    15601 gccaccgcga ttcataatcg tcggcaccgc accttgctcg cagttgatct gattaccgct
    15661 aatacaggcg tcgcagtgac cacagctacg cgccgtccag ccaatcccga cacgctgacc
    15721 gacctgcaaa cctttatcct gcgcggcgct cccgagtgcc accacgcgcc caatcacctc
    15781 atgcccggca accagcggat attgtgaaaa tccccattcg ttatcgatca tcgacagatc
    15841 ggaatggcag atcccgcagt aatccacctg cacttcaaca tcttgtggct tcagctcacc
    15901 gggagcgtac tcataaactt ccagttcgcc gcctgcttct tttgcggcat agctttttat
    15961 catcgacatt ttttggtcct tctctggtgt tgtctgggaa gtgtagagca tggcagggcg
    16021 gggtgcctgg agtgtgacaa aggttacaca tcgctgtatg caatgctgaa aatttcagca
    16081 cttagcgagg tgcgagcaag ctggcgcttg catggtggcg tgcgacaggt ataatccaca
    16141 acgttttccg catacctctt cagtgccgaa gtggcgaaat cggtagacgc agttgattca
    16201 aaatcaaccg tagaaatacg tgccggttcg agtccggcct tcggcaccac accgtatgca
    16261 aatcgagtcg ctcaagggcg gctttttttg tgtctgaaat cacccatatc cgtgcagtat
    16321 ataggcctgc cagaaaagca gttcagtatt cttcattttg tagtcgatgg tcccaagttt
    16381 gctctggctg gaagctgcat cgtccacatt acgtgggcag ggtggtttcg ctatgctact
    16441 tgaacgtttt gaccatgtgg ctcaggtagc taatatcaaa agacgtcatt ttgtctccac
    16501 gcccaccttg gtgaacattc aataaatagt ttcggtcgag agcagttatg tagggctagc
    16561 agacgagatg cgcagacatc ttgttgaaga caattttact gaccacctga gaacgctatt
    16621 ctaccgaata atatttatta tcttggcgaa taatgatgat tacctgtgta actatggttt
    16681 tatgctgatg ggaccggcag tacaatttct gtagtgtact cgcaatttca gatctctggc
    16741 gtttattctg gtgcaaagga ggtatgtgat gaaaactttg ctaccatgcc attaaatgcg
    16801 accaatatcg gattatccat cagttgcttg ttgtagattt cattggttat aaaaatgca
//
LOCUS       DAZGBC010000105        16724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGBC010000105 DAZGBC010000000
VERSION     DAZGBC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.106"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(78..230)
                     /locus_tag="ACHK1J_003326"
     CDS             complement(78..230)
                     /locus_tag="ACHK1J_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7912559.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(495..1229)
                     /gene="cysH"
                     /locus_tag="ACHK1J_003327"
     CDS             complement(495..1229)
                     /gene="cysH"
                     /locus_tag="ACHK1J_003327"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7912560.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1303..3015)
                     /gene="cysI"
                     /locus_tag="ACHK1J_003328"
     CDS             complement(1303..3015)
                     /gene="cysI"
                     /locus_tag="ACHK1J_003328"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO7912561.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3015..4814)
                     /gene="cysJ"
                     /locus_tag="ACHK1J_003329"
     CDS             complement(3015..4814)
                     /gene="cysJ"
                     /locus_tag="ACHK1J_003329"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO7912562.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAEKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVESEVHVTVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5133..5495
                     /gene="queD"
                     /locus_tag="ACHK1J_003330"
     CDS             5133..5495
                     /gene="queD"
                     /locus_tag="ACHK1J_003330"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO7912563.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5573..6844
                     /gene="ygcN"
                     /locus_tag="ACHK1J_003331"
     CDS             5573..6844
                     /gene="ygcN"
                     /locus_tag="ACHK1J_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7912564.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDFPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTYPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHCE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYWAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6835..7095
                     /gene="ygcO"
                     /locus_tag="ACHK1J_003332"
     CDS             6835..7095
                     /gene="ygcO"
                     /locus_tag="ACHK1J_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7912565.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7112..7687
                     /gene="ygcP"
                     /locus_tag="ACHK1J_003333"
     CDS             7112..7687
                     /gene="ygcP"
                     /locus_tag="ACHK1J_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO7912566.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7835..8695)
                     /gene="ygcQ"
                     /locus_tag="ACHK1J_003334"
     CDS             complement(7835..8695)
                     /gene="ygcQ"
                     /locus_tag="ACHK1J_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7912567.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTISVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIDADCQ"
     gene            complement(8692..9471)
                     /gene="ygcR"
                     /locus_tag="ACHK1J_003335"
     CDS             complement(8692..9471)
                     /gene="ygcR"
                     /locus_tag="ACHK1J_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO7912568.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAAELRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCRQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9449..10858)
                     /gene="ygcS"
                     /locus_tag="ACHK1J_003336"
     CDS             complement(9449..10858)
                     /gene="ygcS"
                     /locus_tag="ACHK1J_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7912569.1"
                     /translation="MMGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLILAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10880..12334)
                     /gene="ygcU"
                     /locus_tag="ACHK1J_003337"
     CDS             complement(10880..12334)
                     /gene="ygcU"
                     /locus_tag="ACHK1J_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO7912570.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12404..13189)
                     /gene="ygcW"
                     /locus_tag="ACHK1J_003338"
     CDS             complement(12404..13189)
                     /gene="ygcW"
                     /locus_tag="ACHK1J_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7912571.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13508..14785
                     /gene="yqcE"
                     /locus_tag="ACHK1J_003339"
     CDS             13508..14785
                     /gene="yqcE"
                     /locus_tag="ACHK1J_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO7912572.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVLQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADIAPTTANSK"
     gene            14812..16290
                     /gene="ygcE"
                     /locus_tag="ACHK1J_003340"
     CDS             14812..16290
                     /gene="ygcE"
                     /locus_tag="ACHK1J_003340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO7912573.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLMHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     NAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4004 a         4395 c         4181 g         4144 t
ORIGIN      
        1 aaatataaat tttaaaaagg gtgcaggaga ccaaaccatc acaattcagt acgcggggtt
       61 ttacccccgc gtggtggcta cttcttcgat tcgtaagcga gaactgcctt aaactccata
      121 ccacgttctc tgatactcaa ctcacacaac gagtcgccta ccatcagcgt gaatcctagt
      181 actgtgcaac acagtacgat gattgccaca agggcatatt ttgtcagcat gtcttgcctc
      241 cctgcaaaga agagactaac attcgaattg tttgggttcg aacgtaagcc tcaggttgat
      301 agaaatatcg cctggggctt ttgtccatct ggaacctcgc gaatgcttaa cgccagacag
      361 cctcaagcac ccgacgccat tctatacctg ataattcttc cagcggcgtt cacgtttaaa
      421 atcaggaatg ctgctcgcaa aataaaaacg cccccatcat ttctgacaga ggcgtttaat
      481 ttatccggca atatttaccc ttcgtgtaac ccacattccc tttttaagcc aaagaaacgc
      541 gtttcttctt ccgccatgcc gggttcccat ttacgggttg tatgggtatc gcccaccgat
      601 aaatatcctt cgtcccataa tgggtgatat ttcaggccat gcttttgcag atactgatat
      661 atcgttcggt tatcccagtc gataatcggc agcactttaa atacgccacg ctgaattgcc
      721 agcaccggta aattggcgcg gctgccggat tgttcgcggc gcaggccagc aaaccacgtt
      781 tgcgcgttaa gttctttcag cgcccggttc atcggttcga ctttgttgat gtcattgtac
      841 ttttcaatgc cttcaacgcc ctgttcccac agttttccgt agcgtgcttc ctgccaggct
      901 gcgctttcgg tagcacggta cactttcagg ttgagcttga gtttgtccgt taactcgtca
      961 ataaagcggt aggtttccgg gaacagataa cccgtatcgg tgaggatcac cgggatatcc
     1021 gggcgaatct gattcaccag atgcaggctg accgccgcct gaatgccaaa gctggaagaa
     1081 agcacatatt caccgggcag attatccagc gcccaggcta cgcggccttc agcgtccagt
     1141 ttttccagtt cggcgttagt ttctgccagc gccagaatgc gatctacctt cggcagttcg
     1201 ttcagggcgt ttagatcgag tttggacata gattcctcac tgtttgcctt gcctgatgcg
     1261 acgctcacgc gtcttatcag gcctacaaga ccgggctgat ggttaatccc acaaatcgcg
     1321 cgccggatcg agcaccgggc gaatgatgcc cgcacgcacc gtaaagtcgc cgaagccttc
     1381 acccgcttcg cgctctttcg cccagcgccc tatcagttca tcaagcgacg ccaggatttc
     1441 cggctcggtg atgttttctt tatacatccg tgggatacgt gtcccaatac ggttgccgcc
     1501 aagatgcagg ttgtagcgac ccggcgcttt acccaccagg cccacttccg ccagcatcgc
     1561 gcgaccacaa ccgttcgggc agcctgttac acgcatcacg atatgctcat cgctgacacc
     1621 atgtttcgcc attaaattat cgatgttgtc gataaaagac ggcaggaaac gctccgcttc
     1681 cgccatcgcc agcgggcaag tcgggaacga gacgcaggcc attgagtttt cacgctgcgg
     1741 cgtgacggca ttcattaacc cgctctcttt ggcgatcttc tcgatcttcg ctttctcgct
     1801 ttccggcaca ccggcgatga tcagattctg gttcgccgta atgcggaaat cacctttgtg
     1861 gatcttcgcg atctccagca ggccggtttt cagcggacgc cccggataat caaggatgcg
     1921 accattttcg ataaacagcg tcaggtgcca gttatcatca atgcccttaa cccagccaat
     1981 acgatctccg cgtccggtaa actcatacgg gcgaatcggt tcaaatttga tccccgcgcg
     2041 acgctccact tccgctttaa acgtctcaac ccccacgcgc tccagcgtgt atttggtttt
     2101 ggcattttta cgatcggttc ggttacccca gtcacgctga gttgtcacga cggcttccgc
     2161 caccgccagc gtatgctcca gcggcagata gccaaactca ctcgccgtgc gggcgtaggt
     2221 tttcttgttg ccgtgttcga tagaaagccc accgcccacc aacaggttaa agcccaccag
     2281 cttgccgttt tcggcgatcg ccacgaagtt catgtcgttg gcgtgcagat cgatatcgtt
     2341 ctgtggcggg atcactaccg tggttttgaa tttacgcggc aggtaggtct ggccgaggat
     2401 cggttcttca tcagtggtgg cgactttttc ctgatcgagc cagatctccg catacgcgcg
     2461 ggtacgcggc agcagatgtt cagagatttt cttcgcccac tcgtacgctt ctgcgtgcag
     2521 ctgcgactcg taagggttcg aggtgcagag tacgttacgg ttcatgtcgt tagcggtcgc
     2581 cagcgcatca agaccgaccg agtgcagcat ctggtgcacc ggtttgacgt tctttttcag
     2641 aatgccgtgg aactggaatg tctggcggtt ggtcaggcga atgctgccat agatggtgtt
     2701 ttcaccggca aatttatcga tcgcctgcca ctgtttggtg gtgataactc cacccggcag
     2761 acggcagcgc aacagcatcg cgtggcgcgg ctccagcttc tgttcagcac gttcggcgcg
     2821 gatgtcgcgg tcatcctgct gatacatgcc gtggaagcgg atcagcagga agttatcgcc
     2881 cttaaagccg ccggtcagac cgtcgtttaa atcttccgca atggtgccgc gcaggtagtt
     2941 gctttcaagc ttcatgcgct cggcgtctgt cagttttcct tcgaccacta aaggccctgg
     3001 atgtttttcg ctcattagta gacatctcgc tgataacggc gctctacgcg cagctcactt
     3061 aaaaattcat ccgccgcttc ggtgtccatg ccaccaaatt cggcaatcac ttccagaagt
     3121 gcctgctcaa cgtctttcgc catgcgatta gcgtcgccgc agacataaat gtgggcaccg
     3181 tcattgatcc agcgccacag ctccgcgccc tgttcgcgca gtttgtcttg tacgtaaact
     3241 ttttcttttt gatcgcgcga ccaggcgaga tcgatccgcg tcagcacacc ctctttgacg
     3301 tagcgctgcc actccacctg atacaggaag tcttccgtaa aatgcggatt gccaaagaac
     3361 agccagtttt tacctggcgc ttcgtcagcg gcgcgttgct gcataaaggc gcggaacggc
     3421 gcaataccgg tgcctgggcc tatcataatc accggggttt ccgggttagc gggcagacgg
     3481 aagttgtcgt tatgttcgat aaagacgcgg acttcaccct cttcttccac gcggtccgcg
     3541 aggaagctgg aggcaccacc cgcacgggcg cggccttcaa tgtcataacg aacgacacca
     3601 acggtgacgt gcacttcact ttccacttca gcctgcgatg aggcgatgga atagaggcgc
     3661 ggcgtcagcg ggcgcagcag gttaatcagc gcttcagcat caagctgtgc cggggagaaa
     3721 cgcaccatgt caacaatcgg cgtcgtcgcg gcgtaatgct gtaacttcgc tttatcgccc
     3781 accagcggca gcagtgtttc actgcgggta agcgtggcgt aattctcaac aatgttggcg
     3841 gtgttgacgg tcagttcgaa gtgccactgt agcgcttcgt tcagaggcaa cgttttgccc
     3901 tcgacggtga caggttcatc gcctttcagc cacagcagtt cgacaagttc tttcaccagt
     3961 gccggatcgt tctgatacca gacacccagc gcgtcacccg gctggtaacg caggcccgag
     4021 tcacctaagt caatttcgat atggcgaacg tctttttcag agttacgccc ggtaattttc
     4081 tggttaaccg aaaggctcgc taccagcggc gcgtctttgc tgtacgggct ggtgtggatt
     4141 tcatttacca cgccagtagc gacggattgc gaaggtgccg cgacaggcgc acgcgattta
     4201 agcgcatcaa ccacgcgggc gcgccactcg ctggcagcag cctggtattc aacgtcggcg
     4261 tcgacacggt cgagcaggcg ttcaccgccc agttccgcca gcttgctgtc gaaatctttc
     4321 ccggactggc agaaaaattc ataagaacta tcgccgaggc taaacacggc aaaagcggta
     4381 ttttccagct tcggcgcttt tttggagaac aggaacttat gcagcgcgac ggcttcttcc
     4441 ggcggttccc cttccccttg cgttgacgtc actacgatga gcagtttttc gctggcgatt
     4501 tgtttgaatt tatagtcgcc cgcgttcacc agcttaacgt tcagtttttc tgctaataaa
     4561 tcatcgcgta atgcttcagc aacccggcgc gcattgccgg tttgcgaagc agagataata
     4621 gttatacccg gcatttctgc ggctggcgct ggcgtcgctg caagcgcagc aggctgctga
     4681 ttgagcacgc cccagaaata gccagaaacc caggcaagct gggtgggagt taaatcggtc
     4741 gtggccgcct gaaggcgtgc cagttgctcc gggttcaacg gaagcaacgc ggaaggtggg
     4801 acctgtgtcg tcatgcgtcg ttatgttcca gtaagcaaag ctgtttctgc gccctgtcag
     4861 cgcccataaa acagaagaga aggtaaggtt aacggggtga acggtgtgga ttaaagacgg
     4921 gatagcgata actaataacc aaatcgacta acctgtttta gcaatagtct ttaacaacaa
     4981 aatagattaa acaacctaat gaaaaacaaa tgaatttagc caatcattaa gataaatcag
     5041 cgattttgcg caacaaagtc gttttagata atgcgaaaaa acagcctttc cggtactcta
     5101 cggcggtttt atcgtcctgt agagaaatta tgatgtccac cacgttattt aaagatttca
     5161 ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc
     5221 acgggcattc ctttatggtg cgactggaaa ttaccgggga agtcgatccg catacgggct
     5281 ggattatcga tttcgctgaa ctgaaagcgg cgtttaagcc aacttatgag cgcctcgatc
     5341 accattatct caatgatatt ccaggtctgg aaaacccgac cagcgaagtt ttagcaaaat
     5401 ggatttggga tcaggttaaa cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa
     5461 cctgcaccgc tggttgtatc tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc
     5521 ccttaagtaa cgctatgtta gggtgttgtg ttcaggatat ctggggcatg acatggaaga
     5581 cgactgcgac attattatta ttggtgccgg tattgcaggc accgcttgcg cgttacgctg
     5641 cgcgcgagcg ggtttatccg ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa
     5701 tctttccggc gggcggttat atacccatgc actcgcggaa ctcctccctc agtttcatct
     5761 gaccgcgcct cttgaacgac gcatcactca cgaaagcctt tccctgttaa cgccggatgg
     5821 cgcaacgacg ttttccagct tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg
     5881 gttcgatccg tggctggttg ccgaagccga aaaagaaggt gtcgaatgta tccccggagc
     5941 gacggtggat gcactgtatg aagaaaatgg cagagtgtgt ggtgtcattt gtggtgacga
     6001 tattctccgc gcccgttatg tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg
     6061 tcacggatta gtgactcgtc ctgctggcga agcgatggcg ttggggatca aagaagtgct
     6121 gtcgctggaa acatccgcta ttgaagaacg ttttcatctg gagaataacg aaggcgcagc
     6181 gttgctgttc agcggcggaa tctgtgatga ctttcccggc ggcgcatttc tttatactaa
     6241 tcaacaaacg ctctcgttag ggattgtttg cccgctctct tcccttacgc aaagtcgtgt
     6301 tccggcaagc gagctgctgg ctcgctttaa aacgtatccg gcagtgcgcc cgcttatcaa
     6361 aaacacggaa tcactggagt atggtgcgca tctggtgcca gaaggtggct tgcacagtat
     6421 gccagtgcaa tacgccggta acggctggct gctggtgggc gatgcgttac gcagttgcgt
     6481 caataccgga atctccgtgc gcggcatgga tatggcgctg actggcgcac aggcggcggc
     6541 acaaacgctg ataagcgcct gccagcactg cgagccgcaa aatctgtttc cgctttatca
     6601 tcacaacgtc gagcgcagcc tgctgtggga tgttctacag cgttatcagc atgttccggc
     6661 gcttttgcaa cgccctggct ggtattgggc gtggcctgcg ttaatgcagg atatttcccg
     6721 cgatttatgg gatcagggtg ataaacctgt tccaccgctg cgccagttat tctggcgtca
     6781 tttacgtcgt cacggcctgt ggcatctggc gggcgacgtt atcaggagtc tgcgatgtct
     6841 gtagcccgta atctctggcg cgctgctgat gcgccacata ttgttccggc tgactccgtt
     6901 gagcgccaga cggcagaacg gttgattaac gcctgtccgg caggtctttt ttcgctcaca
     6961 ccggaaggtg acttacgtat tgactatcgc agttgcctgg agtgtggcac ctgccgtttg
     7021 ctgtgcgacg aatcaacact acaacagtgg cgctatccgc cttccggatt cggcatcacc
     7081 taccgttttg gataaataat aaggataatt tatgcccctc ttacacctgc tccgccagaa
     7141 tccggtgatt gctgccgtta aagataatgc cagcctgcaa ctggcaatcg actctgaatg
     7201 ccagtttatt tccgtgttgt acggcaatat ctgcaccatc agtaatatcg ttaaaaagat
     7261 taagaacgcc gggaaatatg cttttattca tgttgatttg ctggaaggcg cgtcaaataa
     7321 agaagtcgtg attcagtttt tgaaactggt gaccgaggcg gacggcatta tcagtaccaa
     7381 agcctcaatg ttgaaagcgg caagagcgga aggttttttc tgtattcatc gcctgtttat
     7441 tgttgattcg atttcgtttc acaacattga taagcaagtt gcgcaatcga atccggattg
     7501 tattgagatc ctgccaggct gcatgcccaa agtgctgggc tgggtgacgg aaaaaatccg
     7561 ccaaccgctg attgctggcg ggctggtatg cgatgaagaa gatgcgcgta atgcgattaa
     7621 cgcgggtgtc gtggcgcttt ccaccacgaa taccggagtc tggacgttag cgaaaaaatt
     7681 gctttgacgg gataatcgta accaattgaa tttggtttga tttgtaggcc gcacgccaca
     7741 tccgacattc agcgcctgat gcgacgctta acgcgtctta tcaggcctac aggtccccag
     7801 tccagaaccg taggcaggat catccggcaa cacgttattg gcaatctgcg tcaatgtttg
     7861 tcaccagtgc ctccagcacc actttccagt catcaaccac gccaacatct gcctgtgaaa
     7921 ataccgctgc gctggcgtca tgattaatcg ccaccacaaa cttgctattg cgtacgcccg
     7981 ccattaacgc tgctgcgcca gaagcgccaa caacaataca cacctcaggt gccagcagat
     8041 gtccggaaat accaatcact ttttcagcat caacgccacc gttcatcact cgtgcccgac
     8101 tgtagcctac ttcggctccc agcttttctg ccagcatagc aatttcctgg ttatctgcct
     8161 ctccgccctg ccccaccacc agcacccgtc tggcctcagc taatgggtcg cgagtaacat
     8221 ttttcagatc ctctgtgcta accagccagt ccggaagcgc acccgggaca atgttcagtt
     8281 gttgcattcc agagggcaac gtggcgtttt tcgctgctcc cgcctgacgc gccagggaaa
     8341 ggcatagcgg gcgtttttct gtttgcagcg tcgccgttag cgcatttccc cagtgggatt
     8401 tgcgcacgct tatggttgga atatctaacg aagtcacctg gcagatgctt gcaccatgta
     8461 aacgccaggc cagccgggtc gacagttcat cgccaaacgt tcccggcgga aagagaacta
     8521 cgtccgcagg cgttcgctgc cactgttcca ccaacgcatc cagcacctgt tccgccacta
     8581 caggctgagg ttcaatctgc cagtgcgcca gggtacaacc gctgaaatcc tgtgccgcca
     8641 gccagcttgc tattgcggca ttctcctgat ttattgtcac aattgcgata ttcatggctg
     8701 catcctttgg cgtaaatagt cctgccatag cttttgcgct ttttctgcta ccgtttgccc
     8761 atcaatcagc gtcgctccac gtcgttgctc tgctcttgcc agttgccgac actgcatcgc
     8821 tggcatttcg gcagcaacag ttttacggat aatttctgct ttccccgcag ccattcgctg
     8881 gcgcattccc ggtactggca aggccacttc accgcactga cgcacggcaa tcaccgcagg
     8941 caagcgaacc cggcaacaac gcaacccatg ttcagtacgc tgttcgaggg tgataaacag
     9001 cgcgtcgaga gtgaaacgct ccacctgggt gaagcagggc cagcccagca tttccgccag
     9061 caaaaatggc gtttgcccat tctgcccttc gctgctttgg cagccggtaa ttatcagatc
     9121 cagcggattc tgacgctgcc attcggcaat atggcgagcg acaaattccg gtgcaaagcg
     9181 cagttctgcc gccgtctcca gcaatacagc ttcctcaaac ccaagagcca tgagatagcg
     9241 cagccagtgc aacgcccgtt catcccccat gctcaacgcg gttaaagaca tcggcgtacc
     9301 gttttttctc tgcgccaaca gcagcgcggc ggcagcctgt tcatcagcac cgagtaaact
     9361 tcgcagtagc gaaacatccg gtccgctatt accctgagcc gccgcctgcc actctttttc
     9421 cgccagcatt ccggcatccg gttcggcttt aaacgctaac agaatgttca ttcgcacctc
     9481 ctacatttcc cgccgccacc agcgggagtg ctttggtttc tggtgcccat agccaggtca
     9541 cgacaaaacc aactaacaac actgccgcca gaatcaataa ggtaacctgc attccccact
     9601 gcgccagcac ccacggcagc aggccagtac ttaccgccgc gccaagtcga ctcatggcgg
     9661 tggcaaaacc gacgcccagc gagcgaatgt cagtaggaaa actttccgca ggcaaaatgc
     9721 ccaccagatt actgactgcc gaaatggtgg tgctgaagag aacaaaaagc agcagcgtta
     9781 atgaactgcc ggaaggcaaa caggccatta ctaccagcgt tgccgccagc agcaaaaaac
     9841 ttcccagcaa aaatttgcga tgtgccagca ggtgcgtcag aactaatccc agcagcgcgc
     9901 ccacaattaa caacgcatta agcatcaggc tggcagtcag cgcatcttcc agaccaatag
     9961 tctgggcgat agttggcagc caggtataaa tcacaaacca tgggattacg aggcagacaa
    10021 agaagacgct gttaaacgcc gtgcgccgcc agtaacgcga agagaacaag gttttgatgt
    10081 gtttatgggt cgccgttacc acttcatcgc ccagtaaaac atggggacca aaatagcgat
    10141 gcacgatagc gtgagcttct gcaaaacgcc cctggcgcag tagccagcgt ggcgattctg
    10201 gcgttcccca gcgtaataac gtaatcaaca acgcgggcag agctgccgat gccagcagcc
    10261 agcgccaggc ctccgggttt tcggaaataa agtgatgtcc ggcaatactt gccagcacat
    10321 agcctacggt ccacaccacg ctgaatgcgc ccagcaaaac accgcgatgg cggcgcgggg
    10381 aaaattcagc cagcaaggtg tgacctactg agtaatcgcc tcccagacca atgccgatca
    10441 aaatgcgcag cccaataaga tgctctggcg tggtggcaaa aaattgcagg aacgaagcga
    10501 gcgtaatcag caaaaagctg aaggtgaaga ttttttgccg accaatatgg tcggagatcc
    10561 acccaagaac caggctgcca agaaacaaac caaggagcgc cgagccgccg atcattcccg
    10621 ccataaacgg cgtcagttgc atggcgggcg taagttgaat aatggcgtaa ccaatgacgc
    10681 cgagaacata accgtcggtc aggtgtgcgc cgaaagtgag cgcagcaatt cgacagtgaa
    10741 aacggttaag cggtaaatca tccattcgca ccggtgaagt gttcatcttt accccttcat
    10801 tgtccggtaa taagaacggc ttctcgcctg agaagccgaa aaggcaacgc cccatcatcg
    10861 cggaacgctg cctgacacat tatttttcaa tcggatagat agtacccgta ttcataatgc
    10921 cgttaggatc gaattgcttt ttcagccctt ccagcaacgc ccacgcgctg ccgtgttcca
    10981 gcttgctcca gtgaacgcga tgtttaccaa tgccgtggtg gtgcaccatc gaaccgccga
    11041 ggcgaatggt ttcttcgcag atgattttgt tgagcggatt gtggtacttg tcgatttcct
    11101 cttccggctt acagttgaca acgttgtagt cgtagacgaa gtacatgttg gtgccgttct
    11161 gatagctatg agaggagtga ccacccagca tagtgatgtc gtcggcgtgc gggaactcgg
    11221 tacgaatacg gttaataacg ctttcgtaga tttcgtggat gcagctccag cagccggaca
    11281 cttcagtggt aaagcccatg ttgccggttt tgaggatttg cacacgttcg gcagccactt
    11341 tatccggtcc ccagttcagg ttgttaaacc aggtttcgat cagcttgctg tccacgcgtt
    11401 ggcattgcgg gtagcgggca acgatttccg caatcccttc gcccgtcgcc ttcgcaatgc
    11461 gagggttacc ttcagccata aagatcagca cgcattttcc gtcggcaaaa tgggtgaagt
    11521 gttgggtgcc atcttcagcg tcatacaaac gagcgatcga cggacgatac ccttccacca
    11581 tgatttcacg caggatgttg aagccggttt tcatgtcttc caggatatag ccgtagaaga
    11641 ggttgttttc cggggtgaat ttaaagattt tcactgttac ttcagtgata tagcacaatg
    11701 caccttcgtt gccgatgatg atgtgacgaa tgtccgggcc agccgcgcgg cgtggcacgt
    11761 ttttaatacg tgtgacggtg ccatcagcca atacggcttc cagaccaacg accatatctt
    11821 cgattgcgcc gtagagtgtg gagaactgcc cgatactgcg ggttgctacc aggccgccca
    11881 tctgcgccag cggctttgac tgcggagaat gccccgtggt gtaacctttt tcacgcaacg
    11941 cgttttccag cacctccagc ggaacgccac attgcgccgt cgcctgcata ttctcaatat
    12001 caatattaat gatttgattc atggcggaac cgtcgagcac caccgagttt tctacaacag
    12061 tttccagccc accttcggtg gcggaagcac cggtacgcgg cacaccgtta attttgtgcg
    12121 cattcataaa attcagcaca cgggatactt gctctgtgga accgagtttt acgaccgctg
    12181 ccggaatcgg caaagtataa atgccatgaa tatccggaaa tttacgaaaa cggtcaatac
    12241 tgttcttctt taatactgtt tcatcggtaa ttacgcgatc tgcaccaaca atttccttta
    12301 gctggtcgac aatcgctgcg cgagataaag acatagtaat tccttcctga taataaaaat
    12361 ggaataatca ttaataagag aaaacagaaa ccaccgttat gggttagcgc actaaataac
    12421 cgccatcgac cactaataaa tgcccgttga cataattcga tgcctgactt gcgagaaaca
    12481 cggctgcgcc cattaaatcc tgagtatcgc cccaacggtt tgccggaata tgatcaagaa
    12541 cgcgctgatt ggtttctgga ttactgcgtg tcgccagcgt aatatctgtt gcataatagc
    12601 caggggcgat accatttacc tgaatattat attgacctag ttcatcacaa taagctttgg
    12661 tgaatccggc aagagcatgt ttagtagcag aatatgcagg tgaccattgt ccacctaagt
    12721 aagagaacaa tgaacagata ttaatgattt taccgctttt ctgcgggatc ataatttttg
    12781 ccgcttcgta gcttaactcg aatgcggcgg tcaggttcac atcaatcatt ggatcccagt
    12841 cggcacgacc gaagtccagc accttattca gcttacaaat accggcattg ttaaccagaa
    12901 tatcaactgt accgaaacgc tcacagcagg cagcgataat cttctgcggc gcgccttctg
    12961 cggtgatatc cacctgcatg aagtccacct caacaccctg attttcaatc atttcctttg
    13021 tttcaccgtt atctttgaca aaacttggaa taaagacatt tgcgcctgct ttggccaacg
    13081 ccatggcaaa tgcctggcct aaaccgctat tcccgccggt aacaattgcg gttttacctt
    13141 tcagggagaa aaaatccatt gagaacgcat tgagagattc gattgacata gttagctcca
    13201 atttccgtaa ggcaaaaaaa aagaaaggta acttcccccg cgagagggaa gttacctttc
    13261 tcattatctc tggtaactgg agataatttt tagcataagg acataactgc taatgtgatc
    13321 gtaatcacag tgtgatattc gcattatcaa atagttttaa aaagcagaag cgtgattact
    13381 ctcacacaac acctctgctt tccatgttat ttcttattgc aattactaga gacctgagaa
    13441 aagtaatcca cctgaaaggg cggcttactt ttctcttttt tttgtctatt acaaatggga
    13501 atacgatatg caacacaact catatcgccg ttggataacc ctcgcgataa ttagttttag
    13561 cggcggcgtt agttttgacc tggcttattt acgttatatt tatcaaattc ccatggcgaa
    13621 atttatggga ttcagcaata ccgagatagg tttaataatg agtacctttg gtattgcagc
    13681 tattattctt tatgccccca gcggcgttat tgccgataaa ttctcacatc gcaaaatgat
    13741 tacttccgcg atgatcatta ccggattact gggtctgtta atggcaacgt atccaccgct
    13801 gtgggtaatg ctctgtattc aggtcgcctt tgcgataacg acgattttaa tgctgtggtc
    13861 ggtgtcgatt aaagccgcct cattgctggg cgatcatagt gagcaaggga aaattatggg
    13921 ctggatggaa gggctgcgcg gcgtcggtgt aatgtcgctg gcggtgttta ccatgtgggt
    13981 cttttctcgc tttgcaccgg atgacagcgc cagcctgaaa acggtcatta tcatctatag
    14041 tgtggtttac atcttgttgg ggattctgtg ctggtttttt gttagcgata acaacaacct
    14101 gcgcagtgcc aataacgaag aaaaacaatc attccagctt agcgacatcc tggccgtttt
    14161 gcgcatcagc accacctggt attgcagcat ggtgattttt ggtgtcttca ccatctacgc
    14221 cattctgagt tactccacca actatctgac cgaaatgtat ggcatgtcgc tggtggcggc
    14281 gagctacatg gggattgtga tcaacaaaat cttccgcgcg ctgtgcggcc cacttggcgg
    14341 cattatcacc acctacagta aagtgaaatc ccctacccgc gtgctccaaa tcctttccgt
    14401 actcggcctg ctggcgttaa ctgccctgct cgtcacgaac tctaacccgc aatcggtcgc
    14461 gatggggatt ggcctgattt tactgctggg attcacctgt tacgcctcac gcgggctgta
    14521 ttgggcctgc cctggcgaag caagaacacc gtcttacatt atgggcacca cggtaggtat
    14581 ttgttcggtg ataggattcc tgccggatgt cttcgtttac ccaattatcg gccactggca
    14641 ggacaccctg cccgcagcag aagcctaccg caatatgtgg ctgatgggca tggcggcgct
    14701 cggcatggtg atcgtcttta cctttttgtt gttccaaaaa attcgtactg ctgatatcgc
    14761 ccccacaacg gctaacagca agtaagccag aaaaattaca ggagaaaaat catgtcgaag
    14821 aaatacatca tagggattga tggcggaagt cagagcacaa aagtggtgat gtacgatctg
    14881 gaaggtaacg tggtttgcga aggcaaaggt ttattgcaac cgatgcacac gccagatgcc
    14941 gataccgcag aacatcctga cgacgattta tgggcatcat tatgttttgc cggtcacgat
    15001 ttgatgagtc agtttgccgg gaataaagaa gatattgtcg gtattggtct gggatccatc
    15061 cgttgctgcc gtgcgttatt gaaagccgat ggcacgccag ctgcgccatt gattagctgg
    15121 caggatgcac gcgtaacgcg tccctacgaa cacactaatc ctgacgtggc atatgtcacc
    15181 tctttttcgg gttatctgat gcatcgctta accggcgagt ttaaagacaa tatcgccaac
    15241 tattttggtc agtggccggt ggattataag acctgggcat ggagtgaaga tgctgcggta
    15301 atggataagt ttaatatccc ccgtcatatg ctgtttgatg tgcaaatgcc tggcaccgtc
    15361 ctcggacata tcacaccaca agccgcactg gcgacacatt tcccggcagg actgccggtt
    15421 gtttgtacca ccagtgataa accggtagaa gctctggggg ccggattact ggatgatgaa
    15481 actgcggtaa tttctcttgg cacttacatc gcattgatga tgaacggcaa agcactgccg
    15541 aaagatccgg tggcgtactg gccgattatg tcttctattc cgcaaacatt gctgtatgaa
    15601 ggttacggta ttcgcaaagg catgtggacg gtgagctggc tgcgcgacat gctaggcgag
    15661 tcgttaattc aggatgccag ggcgcaggat ctttcaccgg aagatttact caacaaaaaa
    15721 gcttcttgtg tgccgccagg ctgtaatggt ctgatgacgg tgctggactg gctgaccaat
    15781 ccgtgggaac cgtacaaacg cgggattatg atcggctttg attccagcat ggattacgca
    15841 tggatatatc gttcgatact ggaaagcgtg gcgctgacgc tgaagaacaa ttacgacaat
    15901 atgtgtaatg aaatgaatca ctttgcgaag catgtaatca ttactggcgg cggttcgaac
    15961 agcgatctgt ttatgcagat ttttgccgac gtgttcaacc ttccggcacg acgtaacgcc
    16021 attaacggtt gtgcaagtct gggggcagcg attaatacag cggtaggtct ggggctatac
    16081 ccggattacg caacggctgt cgataaaatg gttcgcgtga aagatatctt tatgcctgtt
    16141 gagagcaatg ccaaacgcta caacgcgatg aataaaggca ttttcaaaga tttaaccaaa
    16201 cacactgatg tgattctgaa aaaatcgtat gaagtgatgc atggggaatt ggggaatgcg
    16261 gattcgatcc agagctggtc gaatgcgtaa gtgaggaaga ccgggcggga aactgcccgg
    16321 cctgaacata cctgaatggt tatccccgct ggcgcgggga acataagttc gacaacttaa
    16381 aaaatgataa caagtccggt ttatccccgc tgatgcaggg aacataataa aaactttaac
    16441 tagttcggtt tatccctgtt tttacaaggc atattaacta cactcgatgt ctttagaata
    16501 aaagacaata cgaatcgcac tttatcaatt ttcacttaaa gtatgaactg gatatcttta
    16561 ttcataagaa atattcatca acagcgtata gaggcggtta tgtaaaacca ctcattagcc
    16621 tcaaaacaaa gacaaatttc cagatttatt acttaatcaa tgaataacaa gcttaaaaaa
    16681 tacactgtta ttctaaagaa aaaattaaca ggcattaatt aata
//
LOCUS       DAZGBC010000106        16719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGBC010000106 DAZGBC010000000
VERSION     DAZGBC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.107"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..1140
                     /gene="yegS"
                     /locus_tag="ACHK1J_003341"
     CDS             241..1140
                     /gene="yegS"
                     /locus_tag="ACHK1J_003341"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO7912574.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIVLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDNIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1222..1995)
                     /locus_tag="ACHK1J_003342"
     CDS             complement(1222..1995)
                     /locus_tag="ACHK1J_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7912575.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2101..3141)
                     /gene="gatD"
                     /locus_tag="ACHK1J_003343"
     CDS             complement(2101..3141)
                     /gene="gatD"
                     /locus_tag="ACHK1J_003343"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7912576.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIVHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3189..4563)
                     /locus_tag="ACHK1J_003344"
                     /pseudo
     CDS             complement(3189..4563)
                     /locus_tag="ACHK1J_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(4567..4851)
                     /gene="gatB"
                     /locus_tag="ACHK1J_003345"
     CDS             complement(4567..4851)
                     /gene="gatB"
                     /locus_tag="ACHK1J_003345"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO7912577.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4882..5334)
                     /gene="gatA"
                     /locus_tag="ACHK1J_003346"
     CDS             complement(4882..5334)
                     /gene="gatA"
                     /locus_tag="ACHK1J_003346"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO7912578.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5344..6606)
                     /gene="gatZ"
                     /locus_tag="ACHK1J_003347"
     CDS             complement(5344..6606)
                     /gene="gatZ"
                     /locus_tag="ACHK1J_003347"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO7912579.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRTYRYGCAE"
     gene            complement(6635..7489)
                     /gene="gatY"
                     /locus_tag="ACHK1J_003348"
     CDS             complement(6635..7489)
                     /gene="gatY"
                     /locus_tag="ACHK1J_003348"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO7912580.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKKYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7789..8841)
                     /gene="fbaB"
                     /locus_tag="ACHK1J_003349"
     CDS             complement(7789..8841)
                     /gene="fbaB"
                     /locus_tag="ACHK1J_003349"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO7912581.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9098..10375
                     /gene="yegT"
                     /locus_tag="ACHK1J_003350"
     CDS             9098..10375
                     /gene="yegT"
                     /locus_tag="ACHK1J_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO7912582.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLITAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10372..11376
                     /gene="yegU"
                     /locus_tag="ACHK1J_003351"
     CDS             10372..11376
                     /gene="yegU"
                     /locus_tag="ACHK1J_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO7912583.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRGEFTDDTSMALCLADALLEREGNIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            11373..12338
                     /gene="yegV"
                     /locus_tag="ACHK1J_003352"
     CDS             11373..12338
                     /gene="yegV"
                     /locus_tag="ACHK1J_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO7912584.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12312..13058)
                     /gene="yegW"
                     /locus_tag="ACHK1J_003353"
     CDS             complement(12312..13058)
                     /gene="yegW"
                     /locus_tag="ACHK1J_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7912585.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13110..13928)
                     /gene="yegX"
                     /locus_tag="ACHK1J_003354"
     CDS             complement(13110..13928)
                     /gene="yegX"
                     /locus_tag="ACHK1J_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO7912586.1"
                     /translation="MQLRITSRKKLTALLCALVLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPGQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKLIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(13993..14793)
                     /gene="thiD"
                     /locus_tag="ACHK1J_003355"
     CDS             complement(13993..14793)
                     /gene="thiD"
                     /locus_tag="ACHK1J_003355"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO7912587.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14790..15578)
                     /gene="thiM"
                     /locus_tag="ACHK1J_003356"
     CDS             complement(14790..15578)
                     /gene="thiM"
                     /locus_tag="ACHK1J_003356"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO7912588.1"
                     /translation="MQVDLLSFAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVATGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15613..15709)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16317..16642
                     /locus_tag="ACHK1J_003357"
                     /pseudo
     CDS             16317..16642
                     /locus_tag="ACHK1J_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         4166 a         4291 c         4026 g         4236 t
ORIGIN      
        1 ttaatatatt atattgaaga tgccactgtt tagttgaata ttaggtatat gctctttttt
       61 gacatttatc ggtggctgcc gttagtattc gctgtcccaa ttgcaagctc ctggtggtaa
      121 ccactgaatc ctccatactt gaactgactt tttatcctcc gactttcatc ctgttctgac
      181 tccacctttt cttttctgct ctacactatc tacagaccaa tcataaaggc acatacgatc
      241 atggcagaat ttcccgccag cttactgatt cttaatggca aaagtactga caatttacca
      301 ttgcgcgaag caattgtgct gttgcgtgag gaaggaatga cgatccatgt gcgggtcacc
      361 tgggagaaag gcgatgccgc acgatatgta gaggaggccc ggaagttggg cgtcgcaacg
      421 gtgattgccg gtggtggcga tggcaccatt aatgaagttt ctacggcgtt gattcagtgt
      481 gagggggata acatacccgc gctgggaatt ttgccattag gaaccgccaa tgattttgcc
      541 accagtgtag ggattcctga ggcactggat aaggcgctga aactggcaat tgccggtaac
      601 gccattgcga tagatatggc gcaggtcaac aaacaaacct gttttattaa tatggcgaca
      661 ggcgggtttg ggacgcgtat taccacagaa acgccggaaa aattaaaagc cgcgctgggt
      721 ggcgtctctt acatcattca tggcttaatg cgcatggata ctctgcaacc ggaccgttgt
      781 gaaatccgcg gtgaaaactt tcactggcaa ggtgacgccc tggtcattgg tattggtaac
      841 gggcgtcagg ccggtggcgg tcagcaattg tgtccgaacg cgttaattaa cgatggcttg
      901 ctgcaactgc gcatttttac cggcgatgaa attcttccag ctctcgtatc aaccttaaaa
      961 tctgacgaag ataacccgaa tattatcgaa ggcgcttcgt cgtggtttga tatacaagcc
     1021 ccacacgaaa tcacttttaa tcttgatggc gaaccgttga gtgggcaaaa ctttcatatt
     1081 gaaatacttc cggcggcgtt gcgttgtcga ttaccaccag attgcccatt attgcgttaa
     1141 tcaaactatt ctctcttgaa ccagagagaa tccgtaatgc cggatgcggt gtaaacacct
     1201 tatccggcat acagaacaat actacaccgt aattaatttt acccgtgtct tctgcaatgc
     1261 cagtttatcc acctctgaca cagcatcatc ggtaataata atgtcaattt tttcgattgg
     1321 cagtacctga ttaaaaccgc gacgattaaa tttcgatgaa tcgagaacgg caatcacttt
     1381 attagcagcg gtgaccatcg caccgctaat cgaataacct tcattaaagg tcgtaatacc
     1441 attaaccgca tcaataccat cagcaccgac aaacattaaa tcggcattaa tatcctgcaa
     1501 tgaacgctcg gcaatggaac catgcatcga gcgcgtttta tggcggaccg tgccaccgca
     1561 gacgaccaga gtaatgtctt tattttcgga aagggcaaac gctgccggga gactgttggt
     1621 aatcaccgtg atatttttgg cggtcattaa tccttcggcg ataagcattg tcgtacttcc
     1681 gctgtcgaga ataacggtca tcccttcgtg gatcattttg actgccgcct gcgctattct
     1741 gttttttggc gcgctggcga gctgaaatcg ctctttaaac ccgacttcct gggtctcggt
     1801 ttcactatta ccggacatta ttttcgccgc accgccatga aagcgcgtaa ccacgccttt
     1861 ttgttcgaga aagcgcaaat cggcacggat tgtcgcttcc gaggcagcaa atactcccgc
     1921 cagatcctga acaagcacgg ttccctgttc attcactaat tggatgatct tattccttcg
     1981 ctcgaatgaa ttcatcgtca tttacccata ttcaattgtg gctagtgtaa acgaaggggg
     2041 accattggtg aatgatctgt tttgcacaat accgggccag catcacgctg gcccgcggtt
     2101 tcagggaatg agcaacactt tgcccggcat agcattacga gcgatgtcac gcaccgcctg
     2161 ggcgaagctt tcaaagcttc cacggtgaac gattaatggc tctaggctta acttacgttc
     2221 tgtcagcaac cggctcgccg tttcccactc ctgccccggc caagggctgg agtagttcat
     2281 ccaactgccg ataaccgtca gctctttacg caatattttg ccaaacgttg ccgatgttaa
     2341 atgcagatcc tgatgcaacg tccccaccag cgccagttgg gcatgaggac cggcaatctc
     2401 taccgccagt tcgacagttt gcggtacgcc agccgtctcg aggataagct gattaaagcg
     2461 cagctcgcgt aaaacgccct gcatttgcgg cgcgctcatt tcgctactgt taaatgtttg
     2521 catcgcaccg aaagattttg ccagtgctag tttttctgag ctaatgtcga tcgccgtcac
     2581 actctttgct cccagcgcga cagcgcactg aatagccagc aggccaatgg ttccggcacc
     2641 aataataata acgtttttat tctcacaacc ttgcgctaaa tgaaaagcat gcaggccaac
     2701 ggtaatcggt tcaataaaag ccccatcctc aataggcata tccgtgggta gagcaaagac
     2761 atttttccgc ttaacgacaa tatattcggc aaatccacca tcacgccgtg agccaataaa
     2821 atcatatttt gcgcactggg aataaaaccc tttcagacac tctggacaag taaaacaggg
     2881 taataacggc acacaggcaa ccgcatcgcc agggtgtaaa tcatcaacac cggatcctac
     2941 cgcatcaata tagccgctaa attcatggcc taacgttatt ggataataat gtgcaccatt
     3001 tttaaatatc ctgggtaaat cggaaccaca taagcccgag ctggcaattt ttacccgcac
     3061 ctcatcctga tgtttaattt caggaatgac gctttctgca acgcgcacga taccatcagt
     3121 atcattcacc actgatttca taaaaactcc tgattgttaa gggggataac ctccccctca
     3181 ggtaaaaatt attctgcaag aacgactttc tcttgtttaa taaagccacg cgctctacgc
     3241 caggtcatga aaataccggt aagataaatt gcgccgataa taatgaaacc gggaatattt
     3301 tgcggggaga aaacctgaat cagtaaccag gtaatgggag aaccgccctg atccattgaa
     3361 gccaccatac ccccggcttt taacgcccca gcattagccg ccagttgggt gtgcaaacca
     3421 atagtttgcg tcgcgatcca cagggtgatg ctcataatga tgacacccga gattaaggtg
     3481 cggaacagat ttccacgatg caccgcgacc gccatcgcca caaagaagcc gatagtggca
     3541 agatcgccaa acggcagcac ctgattaccc ggcacacaaa cagcaattaa aatggtgagt
     3601 ggaataaaaa tcaggcttgc cgataccacc gccgtgtgcc ccagcagcaa cgccggatca
     3661 aggccaatca ggaactcctg accgccgaac ttcgcctgta aacgactacg ggcctgctta
     3721 gcgatgggtg ttaaaccatc catgatgggt taaaccatcc atgatgggtt taatcacccg
     3781 tggcatcagc agcatcaccg ccgctgtttt taccgccagc tgcaatacac ctttgacatc
     3841 gtaacccgcg aggatgccga taatcagccc catcacaaaa cccacggtga caggctcgcc
     3901 aaacgggcca aatttgcgct gaatatcgtc ggcgctaaat ttaatacggt taacgcctgg
     3961 gattttctcg atgatagcat cgaccagcac cgcaattggc cccatatacg ccgacgtacc
     4021 gtgcgggatt gcaatgcctt ccagctcaaa gaaatttcgg gtatcgcggg caaaccagtc
     4081 gccgagctta taaacaaacg ccgcgtgaat taccacaccc gccatcccta tcatccatga
     4141 accggttgcc agatgcagca acgcgccggt gaaggtcata tgccagatat tccagatatc
     4201 aacatttacc acccgcgtca tacgggtcag taacatcgcc acgttaacca gaatcgcaat
     4261 cggaatcgcc accagcgcaa tttgcgacgc ccaggtcatt ggtgaagagc ccggccagcc
     4321 gacatcgacc acatgcagat tcaggtcgaa attttccgcc atcgctttcg ccgccgggcc
     4381 aatggaatcc agcattaagc caatcacaag gccaatgcca acaaagccaa tcccgatatg
     4441 cagacccgct ttaaagcaat cgcctgcctt catgcctaaa attttagaaa aaataatgat
     4501 gacaatcggc aacatcaccg tagggccgag atcgagaata taacgcatga cttctgaaaa
     4561 cataggtcac ccctgtaaga tagtcagaat tttattttgt aatgcttcga taccgacacc
     4621 agaaacaaaa ggcatgccgt gaactaatgg aatatcgcca aaactacgat ccactttggc
     4681 agtggtgcat atcaaatgca cgccatccat ataggtttct atttcattaa cccgacactg
     4741 gattaattca acaggaatat tatgactctg acacaactct ttaatttctt ccgccgccat
     4801 cgtagaggtc gcaaccgcgc ctccgcaagc gacaataatc ttgcgtttca taaatagtcc
     4861 tttttattgt tacagaggga tttattcgtc tgaatctaaa acatactttg tgaagtattc
     4921 ctttaactga gtttcaggaa gagtgattag tgtctcgacg atatcgggct gttgtaactt
     4981 gccaaataaa cagcgtaaaa gtttcaattg ctgctgcgga ttttccacaa tcaacgcaat
     5041 aaccaacgat accgccacgt cattatcatc atccgcttgc tgaaaatgaa ctttatttgt
     5101 tggccttaac agataaatgg ctgacgactt agcatgaatc gcctcacaat gcggtattgc
     5161 aatggcgtgc tgctcaagca ttatcccagt agggaattct gcttctctgg caattaatgc
     5221 ctgtggccag gtatcatgaa ccacaccttt cgcgagcatc tcattaccga tatgggttaa
     5281 aacttcgcta cgatcgacaa aagaaattcc gctacgaaca aacaggttag tcatataccg
     5341 tccttattcc gcacagccgt agcgataggt gcgcaaaaca tcataaattt tatccataat
     5401 aagctgatgc ggtattgctg ataattcccc ggactgaatg cgttcaaatt gtttaggaag
     5461 atactgacta atcatgccca gtgggatgtc cacgccttcc agattcacca tcatcgtttc
     5521 aacgctattt ttaatccgac tatgcggcca gtaataacga atacgatccg ataggctgta
     5581 acgaatatcc agtaatgaat cgttaaaacc cgtgcgataa tattttttcc agtattgcgg
     5641 ttcgtcgagc atcacttctt caattaccgc caggcaaccg ctgcgatttt cgggggcgat
     5701 aagttcctgc tcaatttgtg ccagtgcaaa tatcgcctcg cgtaaagcaa aggttaatgc
     5761 gggaccgact ttcaatattg caaagtgatc gcggactaat tcccaataag ccgtccgggt
     5821 ctggtaatcg gtagaatgtg cttcataaac cattcgggtg ttttctatcc attgcgccag
     5881 cgcctgcgct tcctgcggct gataatggat aatattgctg tgatcaaatt ccacacccgg
     5941 ctgcaccacg atggcaatca cacgtgttaa cgcctctgtc aggccacggg caataaaggc
     6001 cttttgatgc gtacgtaaag tattggcggc atcttcaaca tgggtgatgt gtactgactg
     6061 aatggcgctg gcctcaccgc ccggaaccgg aacttcggtg ccaatgacat agctcagttg
     6121 ctcacgctgg caatctgtcg ccacactttc cgcagcaaag caaagcacag cagctcgttc
     6181 cgcaaccgtt tctggtgcta acggtatggg atcccccgcg caggacattg acgcatcaag
     6241 atgaatttta ctgaagccag cacgaacata tgcctttacc agctcgacgg atttttccat
     6301 tgccgcatcc gcattttctt gctgccagca gtttggcccc agatggtctc cgccgagaat
     6361 aatgcgctcg cgcgcaaatc cgactttatc ggcaatcgta aaaacaaatt cgcgaaagtc
     6421 tgccggtgtc attccggtat aaccgccaaa ttgattgacc tggtttgacg ttgcttcaat
     6481 cagcactttg cgcgtgctgt tgcgatcaaa tgccagcgcc gcttcgataa ccaacggatg
     6541 ggcagaacag actgaacata tgccgatatg ttcaccagct ttatgccggg caattaacgt
     6601 tttcatgttt tttccttgtt aaatggcagg tgcgttatgc cctgccctcg cagccacaat
     6661 cggcaatcac tttgctcacc acatcgcgca ttgcggattt agccgactgc aaataatccc
     6721 ggggatcggt cgcttcgggg tgctcggtca ggtatttttt taacgcctgc gagaaggcat
     6781 ttttcagctc cgttgcaacg ttgattttgc atatccccag tttgatggtt tgctgaatat
     6841 ccttagtcga taaccctgac gcgccatgca gcaccagcgg taagttcacc cactggcgaa
     6901 tgttctccag tcgagaaaaa tcaagcgccg gtgcgctggc atacatacca tgagccgtgc
     6961 cgatggcgac cgccagggaa tcaattccgg ttgcctcggc aaattcacgc gcctgagcgg
     7021 ggttggtata aaaagcatcg gcttcattga cttgcacatc atcttcctgg ccgccaagtt
     7081 gccccagctc cgcttcgacg ctgacatcaa agcgatggca aaaatccacc acctctttga
     7141 cccgtgaaat attttgcgca aaaggcaaat gcgaggcgtc aatcatgact gagcgcacgc
     7201 cagaacgaac cttctgagcg atatcgtcaa atttcgtgtg atggtcgaga tgaattgcca
     7261 gtggatggtg atattgcttc gccatcgcgc tgaccagcgc caagagattt tctgtaccag
     7321 catgagtaaa tgtgccaggc gttccggcga tgatgaccgg cgcatgcagg ttggcagcgg
     7381 tttctaccac cacttgcatc gtttcgagat tgtgaatatt gaatgccgga accgcataac
     7441 cgccgcgctg tgcgttgttc agcatctgct ttgtcgatac cacgtacatt ttcatatcct
     7501 gtcgtttgtt ttcgatttca aaatataatg aaatgatttg tttttaaatg tcgagataac
     7561 gatcacaaaa acgacaatat gaaaattaat agaggaatga aaggcaaaaa aaaacggcct
     7621 cccgtaggga agccgcaaca aagtgcgcgt ggttttatgc aggatgcggc gtaaacatat
     7681 gtggcagcgc tgcgtaggca tgataagacg cttcagcgtc gcatcaggca taggttgccg
     7741 gatgcggcgt aaacgcctta tccgtcctac ggggtagtgc cgttcgtatc aggcgatagt
     7801 aattttgcta tcgagataaa cgtcctgcac ggcgttaatc agtttcacgc cgtcagccat
     7861 cgatttcttg aacgccttac gtccaagaat cagccccatt ccgccagcgc gtttgttgat
     7921 aaccgcagta cgcactgcat cactgaggtc agtttcaccg cctgcagcac cgccggagtt
     7981 tatcaacccg gcccggccca tatagcagtt agctaactga taacgcacca gatcaatcgg
     8041 gttttcgctg gttaacttgc tgtacacgcg atcgtcggta taaccgtaat taattgcttt
     8101 atagccgccg ttattttccg ccattttttg tttgacgata tctgcaccta tggttgccgc
     8161 cagatggttc gcctgaccag tcaggtcggc ggagacatgg taatcaacgc catctttctt
     8221 aaaggcggag ttacgcaaat aggcccacag cactgtcacc atgcccagct cgtgcgcacg
     8281 ttcaaaagcc gcagaaattt cttcaatctg gcgacgtgac tcttccgagc caaaatagat
     8341 agtcgcacca accgccaccg cacccatgtt gaacgcctgc tccacgctgg catacagcgt
     8401 ttgatcgtag gtattcgggt aacttagcgt ctcgttgtga ttaagtttga cgagaaatgg
     8461 aatgcgatgc gcgtaacgcc gcgataccga cgccaacacg ccgtaagttg acgccacaca
     8521 gttacagccc gcttcgatcg ccagttcaac aatgtttttc gggtcaaagt agagcgggtt
     8581 agcggcaaat gaagctccag cagagtgctc aacgccctgg tcaacgggca gaatagaaag
     8641 atatcctgtg ccagccagac gcccggtgtt gtacaacgtc tgcatattac gtaacaccgc
     8701 tggcgggcga ttattgtcaa tcatcacgcg gtctacgtag tcatgtccgg ggagataaag
     8761 ctggtcagaa ggaatagtca tacaacggtg ctgtaaaagg ttgtcggcgt ctttgccaag
     8821 caactgcgca atatctgtca ttactatgct cccgtaaatt ccgattggat atcggctatg
     8881 gattgtcctg gcccgccttt tgcgggcaat cataatcctg gtcgttacgg gcgagttttt
     8941 ccatcttttg gatgtttttt cagcgttttc tgttggctcg attcatcaga aaaaatgtta
     9001 gcgcggtcaa gttttcaccg caaaggtatt taaaaggtat tattaagtgg tattgtcatc
     9061 gcgtacctta cattacctgt catgaaggaa ttaaaagatg aaaacaacag caaagctgtc
     9121 gttcatgatg tttgttgaat ggtttatctg gggcgcgtgg tttgtgccat tgtggttgtg
     9181 gttaagtaaa agcggtttta gtgccggaga aattggctgg tcgtatgcct gcacagccat
     9241 tgcggcgatc ctgtcgccaa ttctggttgg ctccatcact gaccgctttt tctcggcgca
     9301 gaaagtgctg gcggtattga tgttcgctgg tgcggtgctg atgtatttcg ccgcgcaaca
     9361 gaccactttt gccgggttct tcccgttact gctggcctac tcgctaacct atatgccgac
     9421 cattgcgctg actaacagca tcgcttttgc caacgtgccg gatgttgagc gtgatttccc
     9481 gcgcattcgt gtgatgggca ctatcggctg gattgcctcc ggtctggcat gtggtttctt
     9541 gccgcaaata ctggggtatg ccgatatctc accgactaac atcccgctgc tgattaccgc
     9601 cggaagttct gctctgctcg gtgtgtttgc gtttttcctg cccgacacgc caccaaaaag
     9661 caccggcaaa atggacatta aagtcatgct cggcctggat gcgctgatcc tgctgcgcga
     9721 taaaaacttc ctcgtctttt tcttctgttc attcctgttt gcgatgccac tggcgttcta
     9781 ttacatcttt gccaacggtt atctgaccga agttggcatg aaaaacgcca ccggctggat
     9841 gacgctcggc cagttctctg aaatcttctt tatgctggca ttgccgtttt tcactaaacg
     9901 ctttggtatc aaaaaggtat tattgcttgg tctgatcacc gctgcgatcc gctatggctt
     9961 ctttatttac ggtagtgcgg atgaatattt cacctacgcg ttactgttcc tcggtatttt
    10021 gcttcacggc gtaagttacg atttttacta cgttaccgct tacatctatg tcgataaaaa
    10081 agcccccgtg catatgcgta ccgccgcgca ggggctgatc acgctctgct gccagggctt
    10141 cggcagtttg ctcggctatc gtcttggcgg tgtgatgatg gaaaagatgt tcgcttatca
    10201 ggaaccggta aacggactga ctttcaactg gtccgggatg tggacttttg gcgcggtgat
    10261 gattgccatt atcgccgtgc tgttcatgat ttttttccgc gaatccgaca acgaaattac
    10321 ggctatcaag gtcgatgatc gcgatattgc gttgacacaa ggggaagtta aatgaaaaca
    10381 gaacgtattc tcggtgctct ttatgggcag gcgttagggg atgcgatggg gatgccctcc
    10441 gagctttggc cacgcagccg cgttaaagca cactttggct ggattgaccg ttttctgcct
    10501 ggaccaaagg agaataacgc ggcctgttat tttaaccgcg gcgaatttac cgatgacacc
    10561 tcgatggcgc tgtgtctggc ggatgcgtta ctggaacgtg aaggcaatat cgacccgaat
    10621 ctgattgggc gtaatattct cgactgggcg ctgcgtttcg acgcctttaa caaaaacgta
    10681 ctaggtccga cctcgaagat tgctctcaac gccattcgcg acggtaaacc cgttgctgaa
    10741 ctggaaaaca acggcgtgac caacggcgca gcgatgcgcg tctcgccatt aggttgtttg
    10801 cttccggcgc acgatgttga ttcctttatt gatgatgtgg cgctggcgtc cagcccgacc
    10861 cataaatccg atctggcagt tgcaggtgcg gtagtcatcg catgggcgat ttctcgtgcc
    10921 attgacggag aaagctggtc agcgattgta gattcactgc cttcaattgc gcgacatgca
    10981 cagcaaaaac gcatcactac cttcagcgcc tcactggcag cacgtctgga gattgcgctg
    11041 aaaattgtgc gcaatgccga cggcactgaa tccgccagcg aacagcttta ccaggtcgtt
    11101 ggcgcaggta ccagcactat tgagtccgtt ccgtgcgcca ttgcgctggt tgaactggca
    11161 caaaccgacc cgaaccgttg cgccgtcctg tgcgctaacc ttggcggcga cacagacacc
    11221 atcggtgcta tggcgacggc aatctgcggc gcgttgcatg gcgttaacgc tatcgatcct
    11281 gcattaaagg cggagctgga tgcggtaaat cagcttgatt tcaaccgcta tgccacagcg
    11341 ctggcgaaat atcgtcaaca acgggaggtg gtatgagcgg cgctcgatta cacacgctgc
    11401 tgcctgaatt aaccacgcgt cagccggtga tggtcgtcgg cgcggcggtc attgacgtga
    11461 tcgccgacgc ctacgccctc ccctggcgtg ggtgcgatat cgaactgaaa cagcagagcg
    11521 ttaacgttgg cggctgcgca ctgaatattg ccgtggcgtt aaagcgcctc ggcatcgaag
    11581 cgggtaatgc cttgccgctc ggtcagggcg tgtgggcgga gattattcgc aaccggatgg
    11641 cgaaagaggg gttaatcagc ctgatcgata acgccgaagg cgataacggc tggtgtctgg
    11701 cgctggttga gccggatggc gaacgtactt ttatgtcatt cagcggtgtg gaaaatcagt
    11761 ggaatcgcca gtggctggcg cgattaaccg ttgcgcctgg cagcctgctc tatttttccg
    11821 gttatcaact ggcctcgccc tgcggcgaat tgttagtgga atggctggaa gagctgcaag
    11881 acgtgacgcc gtttatcgat tttggcccac gtattggcga tatcccggat gcattactgg
    11941 cgcggatcat ggcctgtcgc cctttagtgt cgctcaatcg tcaagaggct gagattgccg
    12001 ccgaacgttt tgctttatcc gcagagataa caacacttgg caaacaatgg caggagaaat
    12061 ttgccgcgcc gttgatcgtt cgtctcgata aagaaggcgc atggtatttc agcaacgacg
    12121 cttctggctg cattccggca tttccaacgc aagtcgtcga caccattggg gcgggcgaca
    12181 gtcatgccgg tggcgtactt gccgggctgg cctctggtct gccactggcg gatgccgtat
    12241 tactgggcaa tgcagtggcg tcgtgggttg tcgggcatcg gggcggtgat tgtgcgccaa
    12301 cgcgcgagga actactcctc gcacacaaaa acgtatagat cgctgcgaca gtggctaatg
    12361 ctgtactcaa taggccgttg ctgttggtca agcgccactt gcttgatcac cagcaccggt
    12421 attttgctat ctaactgaat atgtgactga aactcggcat ctggcatccg ggcgctaacg
    12481 cgggaacgtg tacgttgtgg gtaaatgtgc tggctgcgga aatagtcata aagagaaatt
    12541 ccgatggcat caacatcatg aattaaatgc gccggaaccc acgattcctc aatcgatacc
    12601 gcttcttcat cgacatagcg aatacgctta agcaaaaaga catcacttcc cgcttcgacc
    12661 gccagctgct gcgcgacttc ttcgggacat ttcacgacac gcttgttaac ccatagcgta
    12721 tcgggctttt tcccgcgcaa taccacctgc tgagaaaaac ctcgcgcttc tttcaacgag
    12781 tattcgaaga tgttgttgat ctgcgtgccg taaccgcgcg aacgcgtcac cacgccctct
    12841 tcttccagcg cctgcatcgc cttgcgcacc gtaatgcgtg aaacgccggt taactgactt
    12901 aaatcacgct caccgggcaa aatattgcca tgctccagca ccccgctgcg cacggcattt
    12961 tttaccgttt cggcaaactt aatataaagc ggcgtgttat ccgccgctaa aatacgctcg
    13021 ttaagttggg caatcagctg ggtatgcgct tgttccattt gtctttttcc tgccagggag
    13081 tctggtggga gtatatcact gcttcttatt taaggcgttt ctttaatccc atcaacgaat
    13141 gcctgtaact cctccaccgt gccattaaac acattaaaat ccaccggacc attgatgcca
    13201 tctacctgtc cacggtcgga atgctgccag aagcgccagg ccatgccgtc attgtccgga
    13261 cgacgttgat agtagtgagc cacccaccac ggatattcat tgaaatagcc cgccagattg
    13321 gtgtgataaa aaacggctcc tgagtaaata atcagctttt ttcccgtact tttttcgacc
    13381 atttttagcc actgacttac ccgcttgcgt aattcttttg ccgataattt tccccgttct
    13441 tctacgtcca gcacggcagg gaaatcgcct tgtgagaaat ccaccgtttg cagaaataat
    13501 cgcgcctgac caggcgctgc taccgacggg gagaaataat gatacgcccc gcgcagcagg
    13561 ccattttcgc ggcttagttg ccagttacgc gaaaaatagg ggtccaccag cttttcgcct
    13621 tccgtagcct taataaaagc aaactgtaag cggataccgt tgtcgcgcat ttttgccacc
    13681 cgctgccagt cgatccgctc ctgccagcgc gaaacatcta ttccgtgaat ggtgtaactg
    13741 gcaggaatgc gaatagcgaa agatttaacc gggcgataac cgtagaagtg gatgtagtct
    13801 gtaatctgaa ctgctgtcga atagaaaaaa ttcaccgtct gacgcggata aattgcaacg
    13861 atggaaatta gcacaagagc gcataataga gcggtgagtt tttttcgact ggtgattctc
    13921 agctgcattg gtttcatccc tgaatgtcag tgccaaaggc tgacaataac caaagcagct
    13981 atagtacggt gctcaccacc aggcgtggaa gtggtgaacc ggaccaatac cgtgaccaac
    14041 ttccagcgtg tcggcctggg ctaatgctga tgaaagccag ctttttgcct cctgtacggt
    14101 gtcagcccag tttgtatggc gcgggcgtag tgcagccaac gccgcagaga gcgtacaacc
    14161 agtgccgtgg gtgtttttgg tcataatgcg cggtgcggta aaccgttgtt caccctcgcg
    14221 ggtaaacagc cagtccgggc tttgctcatc atccagatga ccacctttca ttagcactgc
    14281 gccacagccc atcgccaaca gcgatcgccc ttgttccagc atttcctgtt cggtgcgcgc
    14341 gtgtggcgcg tcgagcaagg cagcagcttc gggcaagttt ggcgttatta atgaaacctg
    14401 tggcaataat cgactgcgca acgtagcaac cgccgaaggt gaaagcagcg ggtcgccgct
    14461 ttttgccagc ataacggtgt cgagtaccac gttttggatc tgataacgtt gcaaccgttc
    14521 tgccaccgct tcaacaatat cggtttccgc caacataccg attttagtgg tatcgattcg
    14581 cacatcgctg aacaccgaat cgagctgggc ggcgacaaaa tcaggctcaa tgcgatacac
    14641 cgactgtacg ccacgggtat tttgcgccac cagtgcagta ataactgagc aaccataagc
    14701 gccaagtgcc gagaaggttt taagatcggc ctgaatcccc gcaccaccac tcggatcagt
    14761 accggcaatc gtcagagcgt taattcgttt catgcctgca cctcctgcgt caattgccag
    14821 agcgcatcaa ggaaatgtgg aacaaaactg cctggcccct cgcttctggc gactgcgcgt
    14881 tctccggctt gtttcatcca gtgacaggca gatgcgacat tttccagcgt atcgcctggt
    14941 aacgcacagc aggcagcgac aaccgccgat aacgcacagc cagttcctac cactttggtc
    15001 attaacggat cgccaccgtg aataccaatg atacgatgtc catcggtaac ataatccacc
    15061 tcgccagtga ccacgacgat tgcgccagtt tcccgtgcca gtgtttgtgc agcgggtatc
    15121 gcattagctg ccgcgtcagt ggtatccact ccccgtccgc cagtagcaac gccagctaat
    15181 gccatgattt ccgaagcatt accacgtatc gctgccggtt taaaagataa aagttcatga
    15241 caaaaatggc gacgataatc gagcgcacca accgctactg gatcaagcgt ccagggggtt
    15301 tgagagcttt ttgcttgctc aacggcggca cgcatcgcct gtgcgcgtgg ctgcgtcagc
    15361 gtgccaacgt taatcaacaa ggcactggcg atagccgcaa actgactggc ctcttcggtt
    15421 tcgataacca tcgctggcga tgcaccgatc gccagcaagg tattggcagt aaaggtttgc
    15481 accacatcgt tggtcatgca gtgcacaaga ggggaatgtt ggtgaaaaag gtgtaacgtg
    15541 tgcgcagatt gcgcgaaact cagcaggtcg acttgcatag tttgctcctg ccataacgtg
    15601 aagaagcaat gacctggtgg tccgtgactt ccctacgctg gcattatcca gatcaggtga
    15661 tacgggtatt tctcagcctt cacgcagaag ggcaccccga gtcgtttggt tgaatctcta
    15721 tattttgtgc tagattaggg gaagtggctt gcagatgcaa atttaccctc aagtcatttt
    15781 cccggcttac gtggtagctg gcgtagccag cagagaatcg ggtcatataa atatgaccag
    15841 cctgtatcat acaacctttt gtaagaaggc ttcagtatta tcacgccgga ccaagtatgg
    15901 atagaaaaga tcacatacct ttataccata aatgcagttt gtatctgaca atagtgatta
    15961 acctgtagtg atattccatt attggttggt cattttttgc agattactgc atcattagtc
    16021 tgtaacagac tacagataga gaggttggag cgtaagcttc tataaaacgt tgttaagtac
    16081 ttcgtataat cgtgcaattc gcatttagag atcctcaggc ataatcaatc tgccaatgaa
    16141 ggggagcgcc atgcataaag cccgtattac tgaacatcac gccgtgatta tatcgattga
    16201 atccggacaa accgttaaag atgtctcccg ggatgccggt atctctgaag ccatctacta
    16261 caactgaaag tccagattag gcggcatgag ggcttcttat attaaaaaga aaatgactct
    16321 tgatgcacat gggtaatggt ccggaatgta tctttctgag gctgtctgaa cgggactgag
    16381 aaacatggtt taatactgga gtttatcttg ccggataaac cgacaaagaa cgctttcatg
    16441 gagcgtttta ataagacata cttgtacata aatactcgat ttttatctat tcagaacgct
    16501 gaaagaaatg ttggaaatca cggaataatg tttatcagaa tataacagat aataccacat
    16561 gaatcgcaga acaatatttc tgccggagga ataccgatgg cgccattatt ttgtatggtt
    16621 ctcaaaaaat gcatggaact aaaaccgtct attttcttct cgttctagct caaaattttt
    16681 gaagatcatg aaaacaatca aatcggcgaa tagatatgg
//
LOCUS       DAZGBC010000107        16692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGBC010000107 DAZGBC010000000
VERSION     DAZGBC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.108"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..481
                     /gene="ddpX"
                     /locus_tag="ACHK1J_003358"
     CDS             <1..481
                     /gene="ddpX"
                     /locus_tag="ACHK1J_003358"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO7912589.1"
                     /translation="YQQARCLLHKDAITALAKSISIAQLSGLQLVIYDAYRPQQAQAM
                     LWQACPDPQYVVDVTVGSNHSRGTAIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSV
                     PPAAQRNRLLLNAIMTGGGFVGISSEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            495..2045
                     /locus_tag="ACHK1J_003359"
     CDS             495..2045
                     /locus_tag="ACHK1J_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7912590.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASGWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTNKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRNYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            2047..3069
                     /locus_tag="ACHK1J_003360"
     CDS             2047..3069
                     /locus_tag="ACHK1J_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7912591.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGDE"
     gene            3066..3962
                     /gene="ddpC"
                     /locus_tag="ACHK1J_003361"
     CDS             3066..3962
                     /gene="ddpC"
                     /locus_tag="ACHK1J_003361"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO7912592.1"
                     /translation="MMLSEETSAVRPAKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCVFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            3959..4945
                     /locus_tag="ACHK1J_003362"
     CDS             3959..4945
                     /locus_tag="ACHK1J_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7912593.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            4938..5864
                     /locus_tag="ACHK1J_003363"
     CDS             4938..5864
                     /locus_tag="ACHK1J_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO7912594.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWTLRKTELPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIRENGRELRCWRAL"
     gene            complement(5920..6351)
                     /gene="osmC"
                     /locus_tag="ACHK1J_003364"
     CDS             complement(5920..6351)
                     /gene="osmC"
                     /locus_tag="ACHK1J_003364"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO7912595.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            6697..6912
                     /gene="bdm"
                     /locus_tag="ACHK1J_003365"
     CDS             6697..6912
                     /gene="bdm"
                     /locus_tag="ACHK1J_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO7912596.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7014..7151
                     /gene="sra"
                     /locus_tag="ACHK1J_003366"
     CDS             7014..7151
                     /gene="sra"
                     /locus_tag="ACHK1J_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO7912597.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            7308..9005
                     /gene="maeA"
                     /locus_tag="ACHK1J_003367"
     CDS             7308..9005
                     /gene="maeA"
                     /locus_tag="ACHK1J_003367"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7912598.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            9139..10149
                     /gene="adhP"
                     /locus_tag="ACHK1J_003368"
     CDS             9139..10149
                     /gene="adhP"
                     /locus_tag="ACHK1J_003368"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO7912599.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDV
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            10296..10580
                     /locus_tag="ACHK1J_003369"
     CDS             10296..10580
                     /locus_tag="ACHK1J_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7912600.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(10808..11461)
                     /gene="fdnI"
                     /locus_tag="ACHK1J_003370"
     CDS             complement(10808..11461)
                     /gene="fdnI"
                     /locus_tag="ACHK1J_003370"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO7912601.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(11454..12338)
                     /gene="fdnH"
                     /locus_tag="ACHK1J_003371"
     CDS             complement(11454..12338)
                     /gene="fdnH"
                     /locus_tag="ACHK1J_003371"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO7912602.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(12351..15398)
                     /gene="fdnG"
                     /locus_tag="ACHK1J_003372"
     CDS             complement(12351..15398)
                     /gene="fdnG"
                     /locus_tag="ACHK1J_003372"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(14811..14813),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7912603.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            15630..16511
                     /gene="yddG"
                     /locus_tag="ACHK1J_003373"
     CDS             15630..16511
                     /gene="yddG"
                     /locus_tag="ACHK1J_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO7912604.1"
                     /translation="MTRQKATLIGLIAIILWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSMPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         3978 a         4345 c         4289 g         4080 t
ORIGIN      
        1 ttatcagcaa gcgcgttgtc tgttacacaa ggatgcgatt accgcgctgg cgaaaagtat
       61 cagtatcgcc cagctctcag gcttacaact ggtgatttac gatgcgtacc gcccacaaca
      121 agcacaggcg atgttgtggc aagcctgccc agacccgcaa tatgttgttg atgtgacggt
      181 cggttctaat cacagccgtg gcacggcgat cgacctgacg cttcgtgatg agcacgggaa
      241 catcctcgat atgggcgcag ggttcgatga aatgcacgag cgctcccatg cctatcaccc
      301 ttccgtcccg cccgccgctc agcgcaatcg cctgttgctg aatgcgataa tgactggtgg
      361 cggttttgtc ggtatctcca gcgaatggtg gcacttcgaa ttaccccagg cagcgagtta
      421 cccactgctc gctgatcaat tcacctgttt tatatcccct ggcacacagc acgtcagtta
      481 atcaggaaac tgtcatgaaa agatcgatat cgtttcgtcc tacattgctc gcgctcgtcc
      541 ttgccacaac tttcccggtt gcgcacgccg ccgtaccgaa agatatgctg gtgattggta
      601 aggccgccga tccacaaacc ctcgacccgg cggtaacaat agataataac gactggacgg
      661 tgacctaccc gtcttatcag cggctggttc agtacaaaac ggacggtgat aaaggctcaa
      721 ccgacgttga aggcgatctg gcgagtggct ggaaagcgtc tgacgatcaa aaagagtgga
      781 cgttcaccct gaaggacaat gctaaatttg ccgatggcac acccgtcact gccgaagcag
      841 taaaactctc ttttgagcgg ttactaaaaa tcggccaggg gccagcagaa gcatttccca
      901 aagatttaaa gattgatgct cccgacgaac atacggtgaa gtttaccctt agccagccat
      961 ttgcaccgtt cctctacacg ctggcgaatg acggtgcatc cattatcaat ccggcggtgt
     1021 taaaggaaca tgcggcggat gatgcccgcg gcttcctcgc gcaaaatacc gccggttccg
     1081 gaccatttat gctgaaaagc tggcaaaaag gtcagcaatt agttctggtg ccaaatccgc
     1141 attaccctgg caataaaccg aactttaagc gagtatcggt aaaaattatt ggtgaaagtg
     1201 cctcccgtcg cctgcagctc tcccgtggcg acattgacat tgccgatgcg ctgccggtgg
     1261 atcaactcaa cgccctgaag caggaaaata aagtcaatgt ggcagagtat ccgtcactgc
     1321 gcgtcaccta tctgtatctg aataacagca aagcgccact taatcaggcg gatctgcggc
     1381 gggccatttc ctggtctacc gattatcagg gcatggttaa cggcattctg agtggtaacg
     1441 gaaaacagat gcgcggcccg attccggaag gcatgtgggg ctacgatgcg acggcaatgc
     1501 aatacaacca tgacgaaacg aaagccaaag ctgaatggga taaagtgacg aacaaaccca
     1561 ccagcctgac gtttctctat tctgataatg atccgaactg ggagcctatt gctctggcga
     1621 cacaatccag tctcaacaag ctgggcatca atgtgaagct ggaaaagctg gcgaacgcca
     1681 ccatgcgcga cagagtgggt aaaggtgatt acgacattgc gattggcaac tggagtccgg
     1741 attttgccga cccgtatatg tttatgaatt actggtttga gtcagacaaa aaaggtctgc
     1801 cgggtaaccg ctcgttctat gaaaacagtg aggtcgataa gttactgcgc aatgcgctcg
     1861 cgaccaccga ccagacgcag cgtacccgga actaccagca ggcacagaaa atcgtcattg
     1921 atgacgctgc ttatgtgtac ctgttccaga aaaactacca actggcgatg aacaaagagg
     1981 tgaaaggctt tgtgttcaat cccatgctgg aacaggtctt caatatcaat accatgagta
     2041 aataacgtga ccttctggag tattttacgc caacgctgct gggggctggt gctcgtggtg
     2101 gcgggcgtct gcgtgattac gtttattatc tcgcatctga tccccggcga tccggcgcgg
     2161 ttactggcgg gtgaccgcgc cagcgatgct atcgtggaaa atattcgcca gcaactggga
     2221 ctggaccagc cactgtatgt acagttttac cgctacgtca gcgacctgtt tcatggtgac
     2281 ctgggaacat ccattcgtac cgggcgtccg gtgctggaag agttgcgtat atttttcccg
     2341 gcgacgctgg agctggcttt ttgtgccctg ctgctggcac tcttgattgg catcccgctg
     2401 ggcatactct ctgcagtctg gcgaaatcgc tggctggatc atctggtgcg aataatggcc
     2461 attaccggaa tctccacacc tgcgttctgg cttggactgg gcgtcattgt gctgttttat
     2521 ggtcatctgc aaattcttcc cggcggcgga aggcttgatg actggctgga tccaccaacg
     2581 cacgttaccg gcttttatct gctcgatgcg ctgcttgaag gcaacggtga agtattcttc
     2641 aatgcgttgc aacatctcat cttaccggca ttaacgctgg cgttcgttca cctgggaatt
     2701 gtcgctcgcc agatccgctc agcgatgctg gaacaattga gtgaagacta cattcgtacc
     2761 gcccgggcca gcggcttgcc cggctggtat atcgttttat gttatgcgct acccaatgcg
     2821 ttgatcccat cgattaccgt attgggtctg gcgctgggcg atttgttgta tggcgcagtg
     2881 ctcaccgaaa ccgtttttgc ctggcccgga atgggggcat gggtagtgac atcaatacag
     2941 gcgctcgact tccctgcggt gatgggattt gccgtcgtgg tttcgtttgc ttatgtgctg
     3001 gtcaacctgg tagtggattt gctctatttg tggattgatc cgcgtatcgg acgtggaggt
     3061 gatgaatgat gctaagcgag gaaacgtccg ccgtacgccc agcaaaacaa acgcgattta
     3121 acggtgcaaa actggtatgg atgctgaaag gcagtccgct caccgtgacc ggcgcagtca
     3181 tcattgtatt aatgctattg atgatgattt tttcaccgtg gcttgcgacg catgatccca
     3241 acgccattga tttaaccgcc cgccttttgc cgccttctgc ggcgcactgg tttggcaccg
     3301 atgaagtggg acgcgatctg ttcagccgcg tactggtcgg cagtcagcaa tcaattctcg
     3361 ccggattagt cgtggttgcc attgcgggta tgattggttc gctactcgga tgtctatccg
     3421 gtgtgcttgg cggacgcgca gacgccatca tcatgcgcat catggacatt atgctgtcga
     3481 ttccttcgtt ggtactgaca atggcactgg ctgccgctct cgggccgagt ttatttaacg
     3541 ccatgctggc gattgctatt gtgcgaattc ccttttatgt gcgcctggcg cgggggcaag
     3601 cattagttgt acgccagtat acctatgttc aggcggcgaa aacctttggt gcgtcgcgtt
     3661 ggcatctgat cagctggcat attttacgta actccctgcc gccgctgatc gtgcaggcat
     3721 cgctggatat cggtagcgcg attttaatgg ccgccacgtt gggatttatt ggcctgggtg
     3781 ctcaacaacc gagtgctgaa tggggcgcga tggtagcaaa tggtcgcaac tatgtactcg
     3841 atcagtggtg gtattgcgta tttccggggg cagcgatttt gcttaccgcc gtcgggttta
     3901 atctctttgg tgatggtatt cgcgatctgc ttgacccgaa agcaggagga aagcagtcat
     3961 gacccaaccc gttctggaca ttcaacagct gcatttgagt ttccccggtt ttaacggtga
     4021 tgttcacgcg ctcaacaatg tgtccttgca gattaaccgc ggtgaaattg tcggtctggt
     4081 gggagaatcc ggctcaggta aatcagtcac cgcaatgctg attatgcgtc tgctaccgac
     4141 gggcagttat tgcgtacatc gggggcagat ttcactgctg ggagacgatg ttcttaacgc
     4201 tcgggaaaaa cagcttcgtc agtggcgcgg cgcacgagtg gcgatgatct ttcaggaacc
     4261 gatgaccgcc ctcaatccga cacgtcgaat aggtcttcag atgatggacg tgatccgcca
     4321 tcatcaacca ataagtcgtc gggaagccag agctaaagcg attgccctgc tggaagagat
     4381 gcaaatcccg gatgccgtgg aagttatgtc gcgctatccg tttgagcttt caggtggtat
     4441 gcgccagcgg gtaatgattg cgctggcatt ctcctgcgag ccgcaattga ttattgccga
     4501 cgaaccgact acggcgctgg acgtcacggt acagttgcag gtactgcgtt tgcttaaaca
     4561 taaagcccgc gccagtggaa ctgcggtact gttcatcagc catgatatgg ccgtggtgtc
     4621 gcaactgtgc gatagcgttt acgtgatgta tgccggaagc gtaattgaaa gcggcgggac
     4681 ggcagacgtt atccatcatc cccggcatcc gtataccatt ggtttgctgc aatgcgcacc
     4741 agaacatgga gtaccacgcc agccattacc cgccattcca gggacggtac caaacctcac
     4801 ccatttgcct gacggctgcg cttttcgcga tcgttgctat gcggcaggtg cacagtgtga
     4861 aaacgtcccg gcgctgacag cgtgtggtga caacaaccag cgctgcgcct gttggtatcc
     4921 gcagcaggag gtcataagtg tctgacacgt tattaacgtt acgcgacgtc catatcaatt
     4981 tcccggcccg taaaaactgg cttggtaaaa ctacggaaca tgttcatgcc attaatggta
     5041 ttgatttaca gatccgccgt ggtgaaacct tagggatcgt cggcgagtca ggctgcggca
     5101 aaagcaccct cgcacagctt ttaatgggta tgctgcaacc gagccacggg caatacatcc
     5161 gttctggctc acaacgcatt atgcagatgg tgtttcagga cccgctctct tcgctcaatc
     5221 cgcgcttacc ggtgtggcgc atcatcacag aaccgctctg gatagctaag cgtagtagtg
     5281 aacaacagcg gcgagcgttg gcagaggagc tggctgtgca ggtgggtatt cgtccggagt
     5341 atctcgaccg cctgcctcat gcgttctccg gcgggcagcg gcaacgcatc gccattgcca
     5401 gagcactctc ttcgcagcct gacgtgattg tgcttgatga gccaacctcg gcgctggata
     5461 tctccgtgca ggcgcagatc ctcaatttac tggtaacgct acaggtaaat cgcgggctga
     5521 cctatgtgct gatctcacac aatgtctcgg tgattcgtca tatgagcgat cgggtagcgg
     5581 tgatgtatct cgggcagatt gtagaactgg gcgacgcgca acaggtactg acggctccgg
     5641 cacatccgta cacccgatta ttgctggatt ccctccccgc cattgataaa ccgctggagg
     5701 aagaatggac attacgtaaa acggaactgc cgggaaaccg cacgttgccg caaggctgtt
     5761 ttttctacga acgttgccct ctggcaaccc acggatgtga agtccggcaa tcattagcaa
     5821 taagggagaa cggacgtgag ctccggtgct ggcgggcgct gtagagatgc taacaacgca
     5881 tcaggcattc acgcgcctga cgcgtcatcc ggcaatgctt tacgatttca actggtaatc
     5941 cagtgtaatt tccgctttca gtacctgaga gaccgggcat cctgcttttg ctttctggat
     6001 gatgccgtca aaggtagagg catcaatacc cggcaccgca acttcactct tcagtgcgat
     6061 tttcgtaatc gcaaaaccgg catccacttt atccagcgac acatcggcgg tggtatcaat
     6121 cgatgttggc gtgaatcccg cttcccccag cattaatgaa agcgccattg agaaacatgc
     6181 ggcatgcgct gcgccaatca gttcttcagg gttggttcct ttttcgcctt caaaacgcgt
     6241 gttaaatcca tacggctgtt ggttcagcac gccactctcg gtggatactg ttcccttccc
     6301 gcgtttgata tcgccttccc agtgtgcctg acctttctta tggattgtca ttgttgctct
     6361 cctgtgggct taaaaaggaa attatagact acgtgagaaa cagcacgagg ataagcagga
     6421 tattccgaat aaaatccggc agaaaatatt acttctgcac ttaaattaaa tttccacaat
     6481 agataaccgg gaatatatag ctcaagagat attgcatcaa tatcattcaa ctcaccacaa
     6541 taataatacc tatcaatatc atggggttaa aatataaaaa tcatcaacca ggactaatct
     6601 taacaacgaa gcggcaaata tttgccatgc tgaatgtgct ttataaatct gcgatccgta
     6661 gcagacacta taaatacaca gacacggaga atcactatgt ttacttatta tcaggcagaa
     6721 aattcaacag cagaacccgc tctggttaat gccattgagc aaggccttcg ggcagagcac
     6781 ggtgttgtca ctgaagatga cattctcatg gagctgacca aatgggtgga agcttctgat
     6841 aacgacatcc tcagtgatat ctaccagcaa acgatcaatt atgtggtcag tggccagcac
     6901 cctacgcttt aagatgctat gcttgatcgg caacctaatt taggggttta gcacgtgttt
     6961 cttcgctacg gcgatgttgt ccttaaaact agcaacagga ttgaggagtt aaaatgaaat
     7021 cgaaccgtca ggcacgtcat attcttggac tggaccataa aatttctaac cagcgcaaaa
     7081 tagttaccga aggtgacaaa tccagcgtgg taaataaccc aaccggcaga aaacgccccg
     7141 ctgaaaagta attcataacc atcagtcctc aatgacgatt aaacaccatt gcctgcgcaa
     7201 tggtgttttt gtttttatct gctttatact tggggccgac gccctggcgg taaagcaaag
     7261 acgataaaag cgtgccaggg atggatattc aaaaaagagt gagtgacatg gaaccaaaaa
     7321 caaaaaaaca gcgttcgctt tatatccctt acgctggccc tgtactgctg gaatttccgt
     7381 tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac ctgctggggt
     7441 tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg atccagtatc
     7501 agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag gacaccaacg
     7561 aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct gttatttata
     7621 ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt tcacgcggcg
     7681 tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac gtaccaaacc
     7741 ataatatcaa agtgattgtg gtgactgacg gtgaacgtat tctggggctt ggtgaccagg
     7801 gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc tgtggcggca
     7861 tcagcccggc gtataccctt ccggtggtgc tggatgtcgg caccaacaac caacagctgc
     7921 ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgac gaatactatg
     7981 aattcgttga tgaatttatc caggctgtga aacaacgctg gccggacgtg ctgttgcagt
     8041 ttgaagactt cgcacaaaaa aatgcgatgc cgttacttaa ccgctatcgc aatgaaattt
     8101 gttcctttaa cgatgacatt cagggcactg cggcggtaac agtcggcacc ctgatcgcag
     8161 caagccgcgc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt ggcgcaggtt
     8221 cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa ggattaagcg
     8281 aggaagcggc gcggcagaaa gtctttatgg tcgatcgctt tggcttgctg accgacaaga
     8341 tgccgaacct gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac ctcagtgact
     8401 gggataccga cagcgatgtg ctgtcactgc tggatgtggt gcgcaatgta aaaccagata
     8461 ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc cgtgagatgc
     8521 ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa cccgacgtca cgcgtggaag
     8581 ccacaccgca ggacattatc gcctggactg aaggtaacgc gctggtcgcc acgggcagtc
     8641 cgtttaatcc agtggtatgg aaagataaaa tctaccctat cgcccagtgt aacaacgcct
     8701 ttattttccc gggcatcggg ctgggtgtta ttgcttccgg cgcgtcacgt atcaccgatg
     8761 agatgctgat gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc ctgaacggcg
     8821 aaggtctggt actaccggaa ctgaaagata ttcagaaagt ctcccgcgca attgcgtttg
     8881 cggttggcaa aatggcgcag cagcaaggcg tggcggtgaa aacgtctgcc gaagctctgc
     8941 aacaggccat tgatgataat ttctggcaag ccgaataccg cgactaccgc cgtacctcca
     9001 tctaagcctg cgcccggtag tgaaggctac cgggcaattt tcctctccct ttttcagatc
     9061 tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat gcagaacatc
     9121 atccgaaaag gaggaactat gaaggctgca gttgttacga aggatcatca tgttgacgtt
     9181 acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat ggagtgttgt
     9241 ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa aaccggcgta
     9301 attctgggcc atgaaggcat cggtgtggtg gcagaagtgg gtccaggtgt cacctcatta
     9361 aaaccaggcg atcgtgccag cgtggcgtgg ttctacgaag gatgcggtca ttgcgaatac
     9421 tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata cagcgttgat
     9481 ggcgggatgg cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt
     9541 ctggactcgg cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt
     9601 aaactgtcaa aaattcgtcc cggtcagtgg attgctatct acggtcttgg cggtctgggt
     9661 aacctcgccc tgcaatacgc gaagaatgtc tttaacgcga aagtgatcgc cattgatgtc
     9721 aatgatgagc agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc
     9781 accgaagacg tcgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc tgcggtggta
     9841 acagcagttg ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt
     9901 gttgtggctg tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg
     9961 gatggtattg aagtggtcgg ttcgctggtc ggcacgcgcc aggatttaac tgaagccttc
    10021 cagtttgccg ccgaaggtaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc
    10081 aacaccatct ttaccgagat ggaagaaggc aaaatccgtg gccgcatggt gattgatttc
    10141 cgtcactaag aggcctttgc tgcgactgcc agtcctggtg tcgcagcatc gcacactctc
    10201 caacatgaaa tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc
    10261 ccagtgaagg aacactactg agagtaaaga cactgatgaa aatggccaat catccccgcc
    10321 cgggggacat tattcaggaa tcactggatg aacttaatgt cagcctgcgc gagtttgcca
    10381 gagcaatgga aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga
    10441 cgccagaaat ggcaattaaa ctttccgtgg tgattggcag ttcgccgcaa atgtggctga
    10501 atctgcaaaa cgcctggagt ctggcagagg ctgaaaaaac ggtggatgtg tcgcgattac
    10561 gccgtctggt tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca
    10621 aatgcaacat tgcctgatgc gctacgctta tcaggcctac acgttcactg caatctattg
    10681 tatttgaagg gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc
    10741 cacgttgtct acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca
    10801 atagagatta tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat
    10861 accagcgcgg atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt
    10921 taatcgatcc tttcacccag aatgccatat acatatggat caggatggcg tggatcagga
    10981 tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact
    11041 gcgcaaagta cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca
    11101 tgatcgacca gaacatcatc ttttgcccgg cgttgtactt accgacatcc gccactttgt
    11161 gctcattgcc ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg
    11221 ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc
    11281 cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt
    11341 gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca
    11401 cggtccagtg acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca
    11461 tgatgatcct cctcgtcatc gtccacttcc ttattcgggc caataccgat gtaatggaaa
    11521 atcaaccctg caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc
    11581 cacaggctta ccgaggtgtc gatcttcgga tctttcggca gaccgtgata cagctccggc
    11641 tgatcggcgt gatgcagcac gtacataacg tgcgtaccac cgaccccttc cgggttgtag
    11701 acgccagcat gttcgtaacc acgcgctttc agtttcgcca cgcgctgttc cgccagctcc
    11761 agcatctcct tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc
    11821 ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc
    11881 tctttgttga ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg
    11941 cagttttccg actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac
    12001 gggcacgcct tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc
    12061 cactccagct tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg
    12121 ctcagatcgg cggggttatc ataaaccccg acgcagtgcc ccacttcatc acggatgtcg
    12181 ttccactccg aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata
    12241 agtttcgcga cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt
    12301 gcggaccttt tgataatgtc ctgcgtttcc atagccatct gttcgccccc ttacgccttc
    12361 tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc
    12421 ggcgtcagag tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt
    12481 ggaataccca ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt
    12541 accacagcca ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta
    12601 ttaatgcctt tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca
    12661 attgcgttga gcaacgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc
    12721 gtacccacat acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga
    12781 cgaacgaccg ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca
    12841 atcggctcgt agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt
    12901 cccatccctt ccggctgcat aataaacggc ccggttggcg tacccggtgc ggcattgccg
    12961 aagtcaggaa tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc
    13021 ggatcccacg gtttaccgtt gatatccgcc gaagcacggt tgtacagcac gcgacggttg
    13081 agcggccacg cccaggccca tcccagcgta ttccccagac cggacgggtc ggagttatcg
    13141 cggttagcca tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat
    13201 gcggttgtac cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca
    13261 atcagcacgc cattagcatc atagagatct tccagcgcat agccgttgtt ctcttttgcc
    13321 acttcgtcag attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt
    13381 tctacgcctt taccaccttc ggcctggtac agctcgcgca gatgatggta aatacccgcc
    13441 agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc
    13501 aaccagcgac cggagttagc gatagaacca tcttcttcag caaagcaggt cgaaggcaga
    13561 cggaatactt cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc
    13621 cagaaggtag aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc
    13681 aggcagctca ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag
    13741 aaataaccgg tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc
    13801 tggtcccact tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca
    13861 tagaaagatt tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga
    13921 tcagccagcg tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt
    13981 tctgacggca gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcaga
    14041 ccctgaatgt tggagtgacc acgcaatgca ttcacgccgc caccggccat acccatgtta
    14101 ccgagcagca actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc
    14161 gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc
    14221 acttcacaca ctttcaggaa gtcagctttt ggcgtaccac agatgttttc aacaacgtcc
    14281 ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt
    14341 gtttcatcgc gtttcgcata gccgttttca tcgaactgat agttccagga cgatttatcg
    14401 tattgccgtt tttcagcgtc gtagccgctg aacagaccgt cttcgaaagc aaaatcatca
    14461 cgcaccagca agctggcgtt ggtgtaatgc ttaacgtatt cggcgttgat tttgttgttt
    14521 tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt
    14581 gcgtaaatat ccgctacaga agcggtacgc gtaaaacggg gatcgacaac gatcaaggtt
    14641 gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca
    14701 gcgttaccgc ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc
    14761 atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc
    14821 gcctggttgt ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg
    14881 gtttcgttgc tggcaccgga agcacacagc ataccggtag aaagccaacg gtttaccgtt
    14941 acgccctgct cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca
    15001 atacgggaga atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga
    15061 tattccgggt agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttc
    15121 gggcacagcg caccacggct taccggatga tccggatcac cttcaatgtg ataaatcgct
    15181 tctctggcgt ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa
    15241 cagtatgtgc aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga
    15301 gccagtgctt gcttcggggc aaagcccaat gccgctactg ttgttccagc cataccgccc
    15361 gcgcagattt taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg
    15421 tattactgcg cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag
    15481 aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg acctacctca atagcggtag
    15541 aaaaacgcac cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt
    15601 ttaacggcaa aagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc
    15661 tgatagcgat catcctgtgg agcacgatgg taggattgat tcgcggtgtc agtgaggggc
    15721 tcggcccggt cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca
    15781 cggttggatt tccgcgtatt cggcaaatcc cgaaaggcta tttactcgcc gggagtctgt
    15841 tattcgtcag ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc
    15901 aggcgattga agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca
    15961 ttctgtttaa tggtcagaaa accaactggc tgattgtacc tggattatta ttagccctcg
    16021 tcggcgtctg ctgggtgtta ggcggtgaca atgggttaca ttatgatgaa atcatcaata
    16081 atatcaccac cagcccattg agttatttcc tggcgttcat tggtgcgttt atctgggcag
    16141 cctactgcac agtaacgaat aaatacgcac gcggatttaa tggaattacc gtttttgtcc
    16201 tgctaacggg agcaagtctg tgggtttact attttctcac gccacaacca gaaatggtat
    16261 ttagcatgcc cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg
    16321 ctgcatggaa tgtcggtata ctgcatggca acgtcaccat aatggcggta ggttcgtatt
    16381 ttacgcccgt actttcctca gcgcttgcag ccgtgctgct cagtgccccg ctgtcgttct
    16441 cgttctggca aggcgcgctg atggtctgcg gcggttccct gctctgctgg ctggcgacac
    16501 gtcgtggcta aaataattgc cgggtctacg gcccggcaat taatcagata aaccctgaca
    16561 atattaaaaa ataaatttca aataccagta attcacctta tatctcattt cgacttcgat
    16621 cacatatcga taaataatat tatttcatct atttttctca ttgtttttaa tcaccattct
    16681 aatttaaaaa ca
//
LOCUS       DAZGBC010000108        16600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGBC010000108 DAZGBC010000000
VERSION     DAZGBC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.109"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..357
                     /gene="acpS"
                     /locus_tag="ACHK1J_003374"
     CDS             <1..357
                     /gene="acpS"
                     /locus_tag="ACHK1J_003374"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO7912605.1"
                     /translation="TDIVEIARITKAIINGEMAFYERVYTAHERLLINPAEPDYERAS
                     GFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIEQGLQNNSL
                     SISHCRTHAIATVIVY"
     gene            371..1132
                     /locus_tag="ACHK1J_003375"
     CDS             371..1132
                     /locus_tag="ACHK1J_003375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO7912606.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHILQCDLACPQSVSALCEQIEREAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            1136..1684
                     /locus_tag="ACHK1J_003376"
     CDS             1136..1684
                     /locus_tag="ACHK1J_003376"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFO7912607.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAHVGKKLISQGTIINFRETK"
     gene            1706..1987
                     /locus_tag="ACHK1J_003377"
     CDS             1706..1987
                     /locus_tag="ACHK1J_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7912608.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            2023..3183
                     /locus_tag="ACHK1J_003378"
     CDS             2023..3183
                     /locus_tag="ACHK1J_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFO7912609.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCNID
                     GYYLLSEDIPAAELIASMNTILEKAEELDIQSLPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTDEDLLIFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQSVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKAVFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            3257..5815
                     /locus_tag="ACHK1J_003379"
     CDS             3257..5815
                     /locus_tag="ACHK1J_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFO7912610.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVADSERFA
                     KWFENARAAEVLHDIEYSDLNDDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSQARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHLELGSVT
                     FPYDTLEEAKELLAVLDESNQFAELFDEEYSFSDATLPETASNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAAARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALKHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            5820..7043
                     /locus_tag="ACHK1J_003380"
     CDS             5820..7043
                     /locus_tag="ACHK1J_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFO7912611.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHAAKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAITMFAGERQILVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMSNTQAGRAK"
     gene            7040..7993
                     /locus_tag="ACHK1J_003381"
     CDS             7040..7993
                     /locus_tag="ACHK1J_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7912612.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGPFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERRQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            8004..8711
                     /locus_tag="ACHK1J_003382"
     CDS             8004..8711
                     /locus_tag="ACHK1J_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7912613.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            8695..9351
                     /locus_tag="ACHK1J_003383"
     CDS             8695..9351
                     /locus_tag="ACHK1J_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912614.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVKQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVSEWDSEELTQVLLPLMKGE
                     "
     gene            9352..10662
                     /locus_tag="ACHK1J_003384"
     CDS             9352..10662
                     /locus_tag="ACHK1J_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7912615.1"
                     /translation="MSGEILAVNSVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKLRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSTKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            10671..11459
                     /locus_tag="ACHK1J_003385"
     CDS             10671..11459
                     /locus_tag="ACHK1J_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFO7912616.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLVGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            11456..12844
                     /locus_tag="ACHK1J_003386"
     CDS             11456..12844
                     /locus_tag="ACHK1J_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912617.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMNLDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSYSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHHETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLIDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFSLFGD
                     TLGLYLGFKYYL"
     gene            12858..13799
                     /locus_tag="ACHK1J_003387"
     CDS             12858..13799
                     /locus_tag="ACHK1J_003387"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO7912618.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMLNDFAGVDQQRVMHCRKAFTDAKVFK"
     gene            13796..14782
                     /gene="ycdU"
                     /locus_tag="ACHK1J_003388"
     CDS             13796..14782
                     /gene="ycdU"
                     /locus_tag="ACHK1J_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO7912619.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            15149..16351
                     /locus_tag="ACHK1J_003389"
     CDS             15149..16351
                     /locus_tag="ACHK1J_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7912620.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         4183 a         3754 c         4374 g         4289 t
ORIGIN      
        1 acagatattg ttgaaattgc cagaataacc aaagccatta ttaatggaga gatggctttt
       61 tatgagcgtg tttatactgc tcatgagcgc ttactcatta accctgcaga acctgattat
      121 gaacgggcct ctggattctg ggcggcgaaa gaagcgttag ttaaagcgat tggttgtggt
      181 tttcgtgatg gcgtacgttt ccaggatatt gaaataaagc atgatgcagc caaagcacct
      241 tatttcgtta tttccggaag actacaagaa atttttatcg agcaggggtt gcaaaataac
      301 tccctgagta tttcgcattg ccgtacccac gccattgcga ctgtcatcgt ttattaactc
      361 aacatatgtg atgaatatgt acaaatataa tgatttaaat gaaaaaaccg tactggtgac
      421 aggagccagt ggtgatattg gactgggtat ttgcgaaaaa tatctggagc agaactgtga
      481 tgtttatgcg ctctataaat ccaacgacgt gcaattaacg gcgctgaaag caagccatcc
      541 tgcaggcgat aaactgcata ttttgcagtg cgatctcgcc tgcccccaga gcgtgagcgc
      601 attgtgcgaa cagatagagc gcgaagctgg caagattgat gtgctggtga ataacgcggg
      661 catcgttaaa gacagcctgt ttgcctcgat gagctatgag gatttcaccc aggttatcga
      721 aaccaacatg ttcagcattt tccgtctgac gaaagacgcg ctgatgttgc ttcgtgcggc
      781 agaaaatccc gccattatta acgttgcctc tattgcagcg ctgatcccaa gcgtcggtca
      841 ggcgaattac agcgcgtcaa aaggggcgat cctaggcttc acccgcacgc tggcagcaga
      901 aatggcaccc tggggcgtgc gcgtcaacgc ggtcgcaccg ggaatgattg aatcgaaaat
      961 ggtgaaaaaa gtatcccgcg cagtggtccg tgcggtaacc agtaccatcc cgttacgccg
     1021 tctggggaaa tgtgaagaag tggcgaacac catcgtcttc ttaagttcct ctgcctccag
     1081 ctacattgtc ggccagacca tcgttatcga tggcggcctg gtcatgcggt gagttatgtc
     1141 taaatacacg attccttcga aaatcttcct cgaaatgggc ggctggcgtc agccgctgct
     1201 gatggtcgat aaaattgcag attataaata cggtgaaaat ggctttgtca gtgtcgtgaa
     1261 acacgtcacc tacaacgaac cgtatctgct ggggcatttc ccggaagatc cgataatgcc
     1321 cggggtaatt atttcggaaa tcttcggcca ggccagcgag tatctctctt tcctgaccga
     1381 catttgcgac atctggcgcg aacgttttga agaagagctg aagtcgttac gtgatattca
     1441 cgcacgcatt caccgccctg aaatgctgga gattatccgt acccggcgca gccaggtacg
     1501 cggcgttctg gcggctcaga acctgaaatt taaagatatc gcctatccgg gtgattctat
     1561 tgatgttgtc agcaaattgg ctttttcgga tgccagtggt tttaagcact attccgtgac
     1621 cgcgcatgtc ggtaaaaagc tgattagtca gggcaccatt attaatttcc gtgaaaccaa
     1681 gtaatccaat aagagagtaa acactatgtc taccatgaat gacgctattg aacagcgtaa
     1741 agaagttctg atgaccatta aaactgagat catccagcga ctgaatattc aacgcgatga
     1801 agcgcaaatt gatgatgata ctgcgctgtt cggtaatggt tttaaactgg actccgtcga
     1861 tgccacggaa attgtcgtcc tgctggataa agtttttgat atcaagatca gcgaaagtga
     1921 tgacccgtca tatatgcgca gtatcaatag tctggccacc ttcgttattc aaaagaaaaa
     1981 cgactaacta ctttttaaag aattaaatat caggacacgt ttatgaaatc tcttgctgaa
     2041 tttcacttga aaaataatgc ggtaatgggt gtgtataaca accgtaccct accttcttct
     2101 tatcacgatg ctatgactga atataaagct gtacgcgaaa acgcgctgct ggtggactat
     2161 tctcacctct ctatcgttag cgtcatgggc gatgatgcct gggcgctgat caaccagctg
     2221 gtttccgcag atgtctccat cattcgtgat gagcaggcaa tctactcact ggtgttgaac
     2281 gaagagggca ccattcgcgg agacgtctat gtgctgtgca acatcgacgg ttattacctg
     2341 ctgtctgaag atattccggc tgccgaactt atcgccagta tgaataccat tctcgaaaaa
     2401 gcggaagaac tggatattca atcgctgcct gaaattcagg atatgcggga aaacaactgg
     2461 ggggcaatcc tgctcgaagg cccatactcc tgggagatca tgtctgaaat tcatggcttt
     2521 gacgtgattg gcctgcctta ctacgaatac atgaataccg atgaggatct gctgatcttc
     2581 cgctgtggta aacacggtga gtatgcgtac atgaccatcg gtgagcaggc gaaactggcg
     2641 gagcagtggg aaaaactgtt aaccgtcggt gagaaatacc tgatgcagac cggcggtctg
     2701 gattatcaaa aaatcgtacg cctcgaaaat ccgtgctggg atgccagcct gtgggaaggt
     2761 cagtcggtga atccggttca gttgcagatg cagtgggcgg tgcagtacga caaagatgat
     2821 tttattggca aggacgcggt gacagagctc tcccaggagt ataccggtaa taaactcatc
     2881 ggcatgatcg ctcaggaaga atgcgaaggc attgaggctg gcgatcgtgt gctggtggaa
     2941 ggtcaagacg tgggctatgt cgtgaaagcg gtattttccc cggcattgca gcgtttcatt
     3001 gccctgaccc tgctggaaaa ggattacgcc tggtctgata tcagcggcta cgaaattcaa
     3061 actgcccacg gaattattcc ggcgcaatca aaatgtatgc cgtttattta taacctgagc
     3121 atgttggtta gcccaactga gcacagttat atcgacgcgt caaaaaataa aagcgcagcg
     3181 taatgaatat cgccgcgtga ctatagctta cgcggcttaa attaacccca attggcggga
     3241 ttgccaggta ttattcatga aaacgaacag gaaaagagtt gttattaccg ggctggggat
     3301 tttgtcctca ctggcggaaa atattcagga tttccgggaa gcattattaa ataaaaaaaa
     3361 tggcgttgcg gatagcgaac gctttgcaaa atggtttgaa aatgcgcgtg cggcagaagt
     3421 gttgcatgac attgaatact ccgatctgaa cgatgatgtt atcgattcac tggataacgc
     3481 agcgctctgg gcctacaaag tgggtaaaga tgcgctgagt caggccggcc tgatggatag
     3541 ccgtgacacc ctcaaagaaa caggcatgat cgtcggtgtt tcctccgcgg gtacagaggc
     3601 ctttttaccg ctgttcgaac agcgcatgga agatttctcg ctgcgtaaag cactcttttc
     3661 cggcgcattc tcatcctgtt gttccagcgt ttcaacgctg ctgggcttac aggggggggt
     3721 tgaactggtc gcgaccgcgt gtaccgccag cccgaacgcc gtggggatgg cctttgacta
     3781 catccagaat ggtaaaagca aaaccatgct ggcagtaggt accgagccca tctatctgcc
     3841 gacctttgcc ggattctacg cactgaatgt catgcatcca gacacttgta cgccgttctc
     3901 gggtcagtcc ggtatgtcga ttggtgaagg tgcgggcgct atcgtactgg aagagtatga
     3961 gcatgcagta gcccgtgggg cgaccattta tggtgagatc ctctcttacg ccacttcctg
     4021 cgatgctttc catgagaccg ggccagatcc gcgtgccagc ggtgcggtgc aggtcatgaa
     4081 caaggcaatg gcaaatgcgg gcgtcacacc ggagcaaatc gactacgtca acgcgcacgg
     4141 tacgggtact gaagccaatg accgtatcga aacgctggca atgaaaaagg tctttgctaa
     4201 ccacgataaa ctgctggtga gctccaccaa atcttacttt ggccacaaca ttggcgcagc
     4261 ggggattgtg gaactgattg cctgtctggc aacactgccg gacaaccgcg tactgccgac
     4321 gctgaatttc agccaggcac gaccgaattg cgatctggac tatgtgccga atgaatttcg
     4381 cgatcgcaaa gtgaacatct tcatgaagaa taactatgcc ttcggcggca acaactgctg
     4441 catgatcctg agcatggagc ccgcgtccat tccggtcagt acctatgatt cgaagcgtgt
     4501 ggctatctcg ggcgtcggtg cagtatccgc aattggccac acgctcaacc aggttctgga
     4561 aaacatctgg gcgcaggacc accaccttga actgggtagc gtaaccttcc cgtacgatac
     4621 actggaagaa gccaaagagc tgctggccgt gctggatgaa tcaaaccagt ttgccgagct
     4681 gtttgatgaa gagtacagct tcagcgacgc tacgcttccg gaaacggcaa gcaacttcaa
     4741 aacgttccag gtttccgggc tggagccgcg taaacacctg cgtcgtttcg atcagcgtaa
     4801 agcaacccgt ggcggcacgt ttgccctgat cgcactcacc gaagcgctgg aacaggcgaa
     4861 gcgtaaaatc aaacgcgacg gcgatgagct gggcatgatc atggggatgt cccgcggccc
     4921 gcaggaaacc acctacaaat atctgcaaag cctgaaaccc gatccacgca aagtacgtac
     4981 gtcggaattc cctggctcac tgatgaatgc gatcccaacg ttctgcggga tctctgaagg
     5041 gatcaaaggg tataccacta cgcttgcgac aggggaaaac tcggcgctgg gcgcactgac
     5101 ctacggttat gaaatcgtcc ggcagaacct gcaaccgcag gtattggttg gcggcgcgga
     5161 tgagtacttc ccgtcgatgt cgctgtatat ggatgcggtg acacaaaaga tccacatgac
     5221 ggctgaggcg agcgactatc aaatttacgg taaagatccg cagggttatg ttccgggcga
     5281 gggtgcctgc atgctgctgc tggaagatcc acagttggct gcggcgcgtg gcgctgaggt
     5341 tctcgcggaa gtggtgggct acggtaagtc atgtagtaat acctacttcg acgcctcatt
     5401 gttggaagaa aaatccgcgt cgatggcgct ggccattgaa cgcgccctta agcatgcggg
     5461 catctctgct ggcgatattg atttggtatg tggtaccagc aacggcagcg atgaccattc
     5521 tcgtatcgaa attgacgcca tttacaacac cttcgccgcg caaaacccgg cggtgccggt
     5581 agtgaactac aacgcttgct ttggttttgt cgcctctgcc gctggtttgc ttaacctcgc
     5641 cgtgattact gactgcatta agaagcaggc agtacctgcg attccaaata cggtggaatt
     5701 ctttgatgag cgtgtgaatt ttgtacgcca gtcgatgaaa cttgcgctga accatgtcct
     5761 gcttgttggc gcaacggaag gcggcaacta ctacgcgttc gtcattaagg gataagactg
     5821 tggacaatgc cgctattatt tcgggttaca gcgtgtggat gccgtatgcc gaagactgtt
     5881 cgcagttaat tgacaatctg aaacagggga aacgcgttgc gcgtacaccc tggtttacct
     5941 ccaacgagga agccattaaa tgcgggttta agggtaatcc gtcagtggcg acgttgaaac
     6001 aggtggacga tagcgcattg gacctgctgt ctcagttgat cgacgaagcc ctggaacagg
     6061 caatgctgga taagcattgc ctggccgggc gcaacgttcg cgtttacctg accggtattg
     6121 ggccgcgtat cgacgggctg gattacaaat ctttctataa ttacaacgat gtagaagata
     6181 tcaacttaac gcaatccatc accaatctgc atgccgcgaa gatgtcgcag gacacgattt
     6241 caagccatct tgcgcgcaaa tatcgcttgc agtatttgcc gcctaacatg aactgcacca
     6301 gtaactcgtc actgactgcg gtgcatctgg cgacgcaggg cattgagcag ggcgggattg
     6361 atctcgcgat tgtgctgaac tgttcgaaaa ttaaaaccca ggatatctgg ttcctggaaa
     6421 ctcaatcgat gctggacagc gaacaggttc agccgtttgg tgaaaacagt aaaggtgtgg
     6481 cgttcgcaga aggttttagc gcgctcctac tggaaagcgc tcatcaccgc cgggcgcgtc
     6541 aacaaagtga gggcgtaagg gtgcaaacga cttacaccca aatcagcgcg ggccgcagca
     6601 acgatgcctc ctggcttagc actaacgtgc acaaggtcat gcaggccgcg atgaagcagg
     6661 ctgaaattgc gctggatgat cttgcagcca ttcttccaca cggcaacggt tcggcagtga
     6721 gcgataacgc ggaagccaaa gccatcacca tgttcgcagg ggagcggcag atcctcgttc
     6781 tcgcctataa agggcagatt ggctataccg caaccggatc tggtgttgtc gatctgatca
     6841 ttggccacca ttcgttgacg catcatcaac tgatcgcacc tgttggtaac gacgtcatta
     6901 tcgacagcat ggcttcgctg gtacttacgg acggcagcgt gacaaaccat agcaaacgcc
     6961 atttgctgaa ggttggtgtg ggtgtcgatg gttcagttat tggcgttgtc atgtcaaata
     7021 cgcaagcggg gcgcgcgaaa tgacggaaaa gagtatctac ctgtcttcct gggcgcttac
     7081 ggaaccggac gacatttttt atttttatca gccgacctgg ctcaacgcct gggaagcaca
     7141 tatgtacgaa agaggattat atggcactcg tgctaatggc tgggggctga agcgctttgg
     7201 cgttgagaac gatatttatg cgccggaacg gatccgtaat ccacaatata tgccttacct
     7261 tgaggcgatg gagcaggtaa ccgattctgc attacaacaa atgccgacta aagagcgcat
     7321 gaagttaatg aaatcgcgca cggcatttat ttatgccgat tcctgggggg aatccggtcc
     7381 gtttgaaaaa atttccagtg cgctgcatat cgccactatt gatacgcttc ccaagaattt
     7441 acttaagaag ttttcgataa cggaaccgac ttgcaagatt cgcggcgaaa aacaggcatt
     7501 cgttcaggcg ctgcgtatgg cgcaggatta tctcgcctgg gatgtattcg ataacgtggt
     7561 aatttgtgct gcctatcggg caattccggt gctggtattc tctgaagaag atgtgggtaa
     7621 accgcctaag cgtttatttc cccgtcgtgc tgatgaggtg aatttatctg ttgaacgagt
     7681 gggttgtttt attctcagcc agcgtgagag cgctatcagg ctgcaatgcg gggaatatca
     7741 ttccggcact gcgccacagc atgctacgga tattgatgtc atggccttca gcgaacggcg
     7801 acaaatgccg tcccggcctg ctgctggcgt tatcgacctg gtagcccatt acggagaaag
     7861 cggttgtctg acgcccgcgc tgagtatgga ttatttaacc cgtcatattc agcccggtgg
     7921 aaaaatgcgt acggtcattg ccgataaaca gtttggttat cactactttg accttgaata
     7981 ccttgaagga taacgacagt cgtatgcaat ctactattaa atcgttgccc gctatttcgc
     8041 ttagcaacat ttacaagtgt tatggcgctg gggcaggaaa ggttgatgcg cttaaaagca
     8101 ttaatcttga tatcaataaa ggcgaattcc tcgcactgtg tgggccgtca ggcagtggta
     8161 aaagtactct gttgaatatt ctctcgggta ttgataaacc gaccagcgga acggtgatat
     8221 ttttaaataa attactgaac caactgccag aagagcagtt ggctgtgatt cgtggaaaac
     8281 atttgggttt tatttttcag ttttttaacc ttatgccggt attaaatgtt ttcgataacg
     8341 tttatttccc actggtgctt aatggtcagt tcagtaaaaa agaagccact gaacggacgc
     8401 tgcattatct cgatagtgtt ggattaagcg gttttggcga tcgtaaacct gggcaactat
     8461 cgggcggtca acaacagcgt gtcgccatcg ccagggcact ggcccacgaa ccgatggtgg
     8521 tcattgccga tgaaccgacg ggaaatttag acctggcaac cggcgaggct attttggacc
     8581 tgttgttgac gattaatcag cagaccggca ccacatttgt tatttccacg cactccagcg
     8641 aattaaaagc gcgggcacgg cgagtggttg aaattcagga tggagtattg attcatgact
     8701 cagatgcata aaggattttt gctcatcggc gtctttttgt acgcggcgtt tgtggctccg
     8761 aagatttacg cggcaacccc cgacgctgtt aaacaggttc tgcaacaggg gctcccggcg
     8821 cagcctgccg cgctggcgac gctgaccgag gcgttaaaga aagagtatga agccaggcac
     8881 aatgtggtgg cgctggtttt ttacgcctat ggcttactgc gtcaagcgga cggttatgcg
     8941 gcaacaaacg attttatcca tgcctcagaa tacgccaaaa gcggcttttt ctggctggat
     9001 gaagcggtgg atttgcagga gaaaaatcaa cgcgtacggt atttacgcgc ccgcgtagat
     9061 gcctatttac ccgcagactc agggcgttgc gtggtgacag tcaaagacac cgaacacatg
     9121 ctggctgacc cggcgatatg gacgacaacg atccgcgacc atattctggc gatgcgttat
     9181 cgcgcattac gtcactgcaa ggacaccacc cgcgcgaatg cgttgttggc gcaaattaaa
     9241 gggcaaaacg ccgcgcttgc gcagtcgctg acgcaggatt ttaatgttgt atcagaatgg
     9301 gatagtgaag agttaaccca ggtactgctg ccgctgatga aaggtgagta aatgtccggg
     9361 gaaattttag ctgttaatag cgttctgctg ctgatcctgt tttttgtgtt ttatcagctt
     9421 ctgttttatt cacgagatgt caaatttgca tttttgaatc tctttcgcca caaacgtcgc
     9481 tctttttcta ccataaccgc aattgtattg ggtggtgtgg cgatcttttt atatggcgga
     9541 tttattgatt actctttctg gatcctgaaa gagcagacga ttcgtaccaa tattggtcat
     9601 gtgcagattt acaatcagca ctattttgaa acgtcgaata aaaataagag cctgattgcc
     9661 gattatgcgg cgttaaagaa agctattttg tcggacccga cgctggcggc ggatatttcc
     9721 acgctctccg ggcaactgga gtttactggc gtaatttcac attacgagag cgaaacttcc
     9781 agctatttct ccgcgctggg ggtggagccg ttacccgctt taaagctggg ttcgttcgat
     9841 aagattatcg cggggagcga tctttctcgg gtaaaaggtg atgaaatcac cctgggcagc
     9901 ggtctggcga aaacgttgaa tgcgaaatac gacgactggc tggatgtgat ggtggtcaac
     9961 acggcgggcg ggcagggcgc gttgtcgcta aaactgcgtg gcatttttga gtcaggcatt
    10021 aaagattatg acgatgtcgc gatgaagatc ccgctggaca ccgcgcagcg catgatgggt
    10081 acggatggcg tcagcaaagt gctaattctg cttaaagaag atgacaccgc ggcgtttagc
    10141 acaaagctgc gtcagtttat cgaacgcaat catttgccgc tggtggtcaa agactggcgt
    10201 gatgtttcac tcttttatca acaggttgaa gggttgctat cggggatcta tttcttcatc
    10261 aagctgattg ttgcgctgat tgtcatcttc atgatcggca attcgatggc tatgaacatt
    10321 gttgagcgca cccgtgaaat aaccactctg cgtgccatag gcctgaagcc gcttcacgtc
    10381 acgcgcttgt ttctgacaga aggcattttt attggtgtga ttggcgccgt cggcagcctg
    10441 ctggtggggg gcgtgttagc ctggattatt aatctttatg gtattgcgat gccgccatcg
    10501 cctggacaaa ccatcggcta taccgccttt ataaaaacca gtaacccgga attgatttgg
    10561 gtgacggtgg tattacccat tttgacagca acaggcgcct ctgtgttacc ggctttacgt
    10621 gcttcccggc tgaatatttc tgacgcattt aaattctcat agggctaatt atgaatatgg
    10681 ctttttacgg caagtggttt gcttgcctgt ggctcgctac gtcgtgcgtt caggcggcga
    10741 gcacagataa taaggcgctg gagataattc gtcgcgctga cgaaattcgt tcgccgaata
    10801 aaccgtttcg ttacacgctg acggtaacgg aatataaagc gggcgcaacc cagccagaga
    10861 ataaacaggt cctcgatatt tccatgcgct ttatgaaacc gcagggtaat gaaaaagcgg
    10921 atgcacgctc gctggtgcgt tttatttatc cgccgcgaga taaaggaaaa atcatgcttt
    10981 ccgactggta cgatttatgg ttttacacgc cggaactgcg acgtcctatg cctatttcac
    11041 ggcaacaaag attaattggg caaatctcta acggcgatgt cattgtcact aactttgaat
    11101 atgcctatga ctcgaccctg gtgggggaag tcacgtgtgc tgaaaagcag tgttacaagc
    11161 tggcgctggt gcgtaaatcg gcggatatca cctggccgaa ggttatctat tacgttgaaa
    11221 aagacggtga taatcgcccg tggaaggcgg cctattattc gcaggatgac cagttaataa
    11281 aagaagtgtt gtatcaggat ttccagccgg tgttggggaa aacgcgaccc atgaaaataa
    11341 ccgttaccga tgttcgtcac ggcaataact attcagtgat ggagtacagc gatgttcgtc
    11401 tggaatcgct gccggagttt catttcacca aagagtatat tcagcgaggt gcgaaatgaa
    11461 acgtacgctg aagatctctt cgttgctgtg tgttgcgctg ccgttgacgg tacaggcgga
    11521 ttgcctgtca ggtgacgagg tggcacaaaa cagcgatgtg acgctttacg agcaggtgtc
    11581 ttatgtcaat aaacaggctt cggcctggca gattgcaggc aaaaatccct acactcgtca
    11641 caacggttat caggaggcgg ggattggcat caacagtggt tgttcgataa tcgacaatac
    11701 gctggacctt aagctcaatc tgtatggaat aaacgaatat gcgcttaaac ccgcagggaa
    11761 attcgaaacg gatgatagcc gcacacgtgc gttaattaac cgcctcagtt tggtctatag
    11821 cgcctcggac agcgttcaat ttgaagcagg caaatttgcc gcgccgtcag gaatgttctt
    11881 tttgcgttca ccttctgatt tgcaaacgca ttattacacc ggattccagt cgacacgttt
    11941 gcacgatccc aaaatgacat cggcgtatca ggcgtcatcg tggggcgcaa aaatgaactt
    12001 ggatacacgc gattatgcgt tttcggcgag cgtcataccg aagttagcca ccatcgacaa
    12061 acgctatgtg acgtctggca actggtctgc taatcagcag ggaaacagcg acgaagccta
    12121 tctgctgagc tacagcgatc atcgctttgg tgagcacaca ccgacagtca atgtgcggct
    12181 cggaccatcg ccgtcactcg cgctgtctga cagttatcac tacaccccgc agctgacgct
    12241 gaatgtcgac gcagcctacc accgctccca acaatggcgg catctttccc accatgagac
    12301 ggcgcaggtc gaggaatatc agtttccgga ttcgctctat gaaacgaaag atgaaagcgg
    12361 cgtggagctg gcgctgggtg ggcagtacac cagtgataat ttcagcgtgt ttggcgttga
    12421 gtattacttt cagagcgagg gttattcgcg cgctgaacag cgtcagcagc gcgagttaat
    12481 tgacttcctc aataccacaa ccggctatgc gccactggat caggcatttg attcttacaa
    12541 atacctgatg gcttcagaaa tcagcaatac cgccaatcag gggatgctgc agggtaagca
    12601 ctatctgaac gcctgggcca gtctgccgct ggcgggagag tcgacgctgc agccttcgct
    12661 ggtggtcaat ctcattgatg gcagcacgtt gctgggcctg cattattcca cgccgctcag
    12721 cgctatcagt aaccagcttg aagcctatgc cggtggctat agcgcgctgg gaagtcggta
    12781 ttctgaattt tcgctttttg gcgacacgtt aggcctttat ttgggcttta aatactatct
    12841 ataaggataa agagatgatg aataatgctt cacaaccgac agtactgctt tttgccggtc
    12901 agggtaatcc ggttattggc atgggcagcg atctttggga tctcaacgcc agtacaaaac
    12961 atatctggga ttgtgccagc gatatttccg ggctgaacct gcgtcgcctg tgtcagaagg
    13021 ggccgatgaa tcgccttatt caaacgacgg tacaacaact ggcggtgacc gcaatcaatg
    13081 taacgctcta taccctgtgc cgtgaacgtc ttgagaattt aacggttgtg gggtcctgcg
    13141 gacacagcgt tggtgaatac agtgcgctct acgctgccgg agctatttct cttgagacgc
    13201 tatttggcat gattcacttc agagccagcc tgatgcatga cgaaagccag agaaataaag
    13261 gcgcaatgct ggcggttaaa ggcatcaacc gcgaacaact gcagacgatg attacggaat
    13321 caggcattgc gctggatatt agctgtgata acacgccgcg ccagcaggtt attgggggaa
    13381 cgcaggctgc gttgaatgaa tttgccacgc tgctgatggc cgctggatac gaaccggtta
    13441 aactcggtgt gagcggtgcc tggcacacgc gtttgatgga agacggcgtt caggcaatgc
    13501 gcgattatct tgccggactg gatatcgctt cgccagagca tcaggttctg atgaacgtaa
    13561 cagcaaaaag cgaggtcgct ccgtcaatta tcaaagaaaa tctctcatta cacttaactc
    13621 atacggtgaa atggactgaa tctctcgata catttctgaa tatgccaacc tcggtagcct
    13681 tccttgaaat aagtaataag ccctatttgg ggaatatgtt aaacgatttt gctggtgtgg
    13741 atcagcaacg agttatgcat tgtcgtaaag catttactga tgcgaaggta ttcaaatgat
    13801 tcctgattat ttaactttta ttcgctttca ggataaacga agtctgatat acatttatgc
    13861 tattggactt attctgatag gcttttattg gaaaaatgca ggatttactt ttccatcaga
    13921 ggatattggt gtcgttagtg ggattctggc cctggtgctg tataatttta tttttgatct
    13981 caaggcgtac tgggcttata aatgcgtcac gaagaatatc gatttttcgt ggtttaagaa
    14041 aaagcagaac cacaaaatag aattatttct tacgcaacct ctggtggcag gatttctgtc
    14101 gttaatcatg ttgagtgcaa tgagttgggg gctataccag cgtctaccct cgttatatgc
    14161 gctgttcctg atttcgttac ttgggccgtt ggttatcttt ctgttgtttc ggatgatccg
    14221 caccagttat gtcaagcagg tcgctatttc agtggcgaaa aaagtaaaat ataaaagtct
    14281 gactcgctat gtgctgcttt cggtgtgcat ctcaacggtt gttaacttgc ttactatcag
    14341 cccgttgcgt aacagtgatt cttttgtgat agaggggcag tggttaacgt ttaaatcgat
    14401 aattgcattg ctcattcttt gtggcgtagt gttggcgatt aatctgtttt ttctgcgctt
    14461 ctccaggcgg tacgcttttt tgggcaggct ttttttgcag gaaatcgatc tctttttctc
    14521 cagtgaaaat gcgttgtcga ccttttttgc caagccgctt tggcttcggt tattcatatt
    14581 gctggttatt gaagtgatgt ggattacgct ggtgtcggta ttggcaacgc ttgtggaatg
    14641 gcggatttgg tttgaagcct attttttact ctgctatgta ccgtgcttaa tttactattt
    14701 tttctattgt cgattcctct ggcataacga ttttatgatg gcatgtgaca tgtatttccg
    14761 ttgggggcat tttaataagt gaggaagtga taggaagtga ccagataata catatatgtt
    14821 ctgtactctc ttgcgcattt tgattgttga ctgagcaacc agacagttga tgtgcacgat
    14881 ttcccctcgc cctaacagac gtgggcgggg gcacccggtg tgtgcaatat acgaaaaaaa
    14941 agcccgtact ttcgtacgag ctcttcttta aatatggcgg tgagggggac cgcctgaaat
    15001 aaatctgact taatccaggg gtgttatata atttcattat aaacaatgca ttacactttt
    15061 tgttgtccga tattatctaa tgtattctta gtgaagcgag agactaatgg catactggat
    15121 ggcataccag agtgacggga attagcgtat ggcagtattg acggatacga aagcaagaca
    15181 tatcaaacct gatgacaaac cattgcccca tgggggaatt accggactga cccttcatcc
    15241 ttcttcagta aaggggcggg ggaaatgggt ttttcgttat gtaagtccgg tgacacaaaa
    15301 aagacgtaat gcaggattgg gaacttaccc agaggtcagt attgctgaag ctgcacgtac
    15361 tgcccggata atgcgagagc aacttgctgc aggtgatgat cccctggaga ttaaaaaggc
    15421 tgaatctgag aaagtcgtta tcccaacatt tgccgatgca gccaggcgtg tacatgcaga
    15481 actgtctcct ggatgggaaa atccaaagca tgtaaggcag tggttatcga cgcttgagaa
    15541 ttacgcgttt cctcaactgg gagcaaaaac gctggattcg attacggctg cggacgtggc
    15601 agaaacactg cgtccagtct ggttaacctt gtcagaaacg gcaagccggg ttaaacagcg
    15661 cattcatgtt gttatgcagt ggggatgggc gcacggtttt tgtgtggcaa atcctgttga
    15721 tgtggttgac catttgcttc ctcagcagac aagaggacgt gatgaacacc aaccagcaat
    15781 gccctggagg cagttaccgc tttttgtggc gaccagtgtg tatagtgatg aaccttataa
    15841 tgttacccgc gcactgttat taatggtgat acttacagca actcgctcgg gcgaagccag
    15901 gggaatgcgc tgggctgaaa ttgattttca taagcgggta tggactatac ctgcagaaag
    15961 aatgaaagcc aggctacagc atcgtgttcc tttatcccgg caggctattt acattctgga
    16021 aaatatacgt ggcctgcatg atgaactggt gttcccttca cccagaaagc agcagatcct
    16081 ttccgatatg gtgttgacaa gttttctgcg taaaaagaaa gccgtcagtg acattccggg
    16141 gcgagttgcc acggcacatg gttttcgctc aacattcagg gactggtgta gtgaacaggg
    16201 gtattcgcgg gatctggcgg aaagggcgct cgctcatacg ctgaaaaata aggttgaggc
    16261 ggcatatcat cgtactgatc tactggagca gcgtgtaccg atgatgcagg catgggcgga
    16321 ttatgtgatg tctcaaattg tgaataaata atagtgaaaa tgtatattgt acgatcatga
    16381 tttgatatgt gatcgtggca gtagtaatta aggaaccgct tttatgtcaa atgtattgag
    16441 ctgataatta tcagatcgtc aggaaagcgt atttttataa acaggggatt actcaacaat
    16501 ctcctgttta taaaaaacaa tttgcctgtt ttaacgtttc actcctgctg agactgaatt
    16561 attcctagtg cgtcataaat cagtcttctg cgtagtttat
//
LOCUS       DAZGBC010000109        16540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGBC010000109 DAZGBC010000000
VERSION     DAZGBC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.110"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..363
                     /locus_tag="ACHK1J_003390"
     CDS             <1..363
                     /locus_tag="ACHK1J_003390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912621.1"
                     /translation="NILRHSVRNQMAEIKGKITNPALVNAKVFYNRRTKLGIPTEVFL
                     ILMFLNVFAVVLCVMYFSTFSVISLILFVLVSVVPMKFVFHGDPDAHKAWIYGYLSPS
                     RLNNTHAVKRRITFIDAI"
     gene            393..740
                     /locus_tag="ACHK1J_003391"
     CDS             393..740
                     /locus_tag="ACHK1J_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912622.1"
                     /translation="MLNRISRKISTFQIMFYVALVTGSVTPAMAAGNSTVKLPTPDDS
                     FLQKIVNWMQSILDLVGGAGALFFAFVSGFACIVLWGLAPQQSQAIGKLFRAAVAAIA
                     AFNLAFFIAWLQK"
     gene            752..3352
                     /locus_tag="ACHK1J_003392"
     CDS             752..3352
                     /locus_tag="ACHK1J_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFO7912623.1"
                     /translation="MGQSYNEHLNLAGIEQHSGQVYTYTGGAFGAIELTGVDPTGVTP
                     SQGSRISLLLRNIVQLLPLSVTLTQYYIHNDGVKIRLTGRDNQRSQLLSMRRQAYLNK
                     CRNLNASRIIWTLDVHPEENINRLASAEFLKNLFNSMFDDTARRKVRLALSNRESFII
                     ERQAFEKQCLLLKETLRDLDLRLSFISEDNRILDSEGIWRLQKFLATFNHHWLAPRRK
                     VTVPNDMWDCLALEGDDIEAITWRGVDMLKIQGVRPVYVRLASVLKIGDESVPECAWA
                     LTNGRPVLQRGNYVYFMRFTPASAFKKSLMISGKENELVRTQISLSDLMTDSTSKDIL
                     DEKLRRNSHLMKMREELENISYSDERLGICQAGIAVFNTDPDKLLETCADINRSVSQT
                     MQVVWETVAQLDAYKVMQPAYSRNTFRALQLNASQMGAASLFFKSHPGIPEWELNGRM
                     EEAFYILESDDGVPFFFTPKVKEKLLLLGNGATRGGKTFMKNCIASHFIKFGGLYNCL
                     DIDPGSIPMANFFGDDGAAFTLDSDFQCGFNMFSMAQSKDDRVFLAHLIEQLKAMAAE
                     NDSEQDKYLTPDELENITVCASDLLVQKFSPNEGRLSSITLSTLVSKCGQSVKRKLAP
                     FISNGIYGRLFDNKEDAIGVLDKPVAVYNLSGVKDNHNLARLVHREIFFRVVRLFESP
                     EYRTIPKFFEIDEAQYTLSVPHAAEFATQKARTWFKHGGGMGFWTQNPEHYSSLDEWT
                     TLRSSASVFIFMPDNEATVKAFHDAYGFDEEEVNIIRSLKVKQQSYIKIPDMQIAKVV
                     NLFVEAEQYAVCTSTSNEAALAAEIWKRESDPDVAVRQIVEGLITKCNKDLIPTPTET
                     EKDKTEGFFL"
     gene            3349..4170
                     /locus_tag="ACHK1J_003393"
     CDS             3349..4170
                     /locus_tag="ACHK1J_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO7912624.1"
                     /translation="MRKQLAVVLFSVMTGLITTPAISSGIPVADAAQILGQARAMMQD
                     IKNYKEYMEQTILNNKELLEAYKQYNQMLEDYKQILREAEGLKDKLSSIDYKNFMDEI
                     ALISNQFDPVYGGGRMVKNTGNAPWDNAYERNLLLNGYGLTNEEYAEMLAAIPYTSDD
                     RERARQMFEYRQRRVVEGISKDAFVGELQIQLKKQGKTLGELQERRLRLGDNDTTASI
                     QFLAEQNEQLLQQIQSLQLQQAEAMKYSGRLEDHYFNKRAQQEELKARQLQRAWQ"
     gene            4167..4634
                     /locus_tag="ACHK1J_003394"
     CDS             4167..4634
                     /locus_tag="ACHK1J_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912625.1"
                     /translation="MKKYLILFFMMFSASAMAKIGYVDEHQKEVDLKIDALISKYEKE
                     CEGKRNSNMCKSQAWDKAHFEYEDEFRGEDKYNHKHYDGLTKDQAVAKLHELIKLHNI
                     VSKDERNPESWPGKLDTLTINGEINYIVRKHWPAWINPCDKICAELLLRQIGK"
     gene            4694..5665
                     /locus_tag="ACHK1J_003395"
     CDS             4694..5665
                     /locus_tag="ACHK1J_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912626.1"
                     /translation="MSLINRLLFVVLFLSVSFYASAETAMRTNFQNGVSEMVNKILNS
                     PDSDLRVWTIAASTFLAAVTAFVYFWRFVENGGALVDLFTLAIMFMLSMSLLASYSMV
                     VDTITGAFYAISNTLQKLALGNIKPSFLQNFVEGVLTKAISAPSVDIFDGMNMVGMAL
                     LWEICSAILSVAIYLADVWIDVGTAIAKITGVMFIPFLVAPATRGIFDGWFKFYIGWG
                     LAAVVLKITSIVVMIMIRASINSAGSFQGAGDVIASDGTVNGVLNITANNISLLRDIC
                     AYSVIAAIMVFSCFIFAYMIGSGVGSASQGLNNMAVKLAKKAAKALL"
     gene            5675..6394
                     /locus_tag="ACHK1J_003396"
     CDS             5675..6394
                     /locus_tag="ACHK1J_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HFO7912627.1"
                     /translation="MLLEKKEVEKVNRTAFKDRVGELKKDRNLFFWIALLLAGTTIYQ
                     SYSVQEAIEKSQNTTEVVWVKLLPDGSHKISQFTPDNEQPIYTPTVNSLLSKYVQKRY
                     GQLKETIERDYAEAAVFMSPVLTGEFADPKGFNVAQKVVDIQADMAAKRIYIEIKDID
                     HYDAIEGQFDGDVKPVIRTLVTYQEITRDYQGKEISSVQQMRRLQWTLLQRNELSKQS
                     LDWLNINPLGIQIIDDKSVQQ"
     gene            6407..7240
                     /locus_tag="ACHK1J_003397"
     CDS             6407..7240
                     /locus_tag="ACHK1J_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO7912628.1"
                     /translation="MLAKRTLLFSGVLAVVMTTFSATAQQCRVVNWRPGTIVKVQSAL
                     YLGTRIELPEGSMLLTKEPFSSNALWASTGAANQVMIQPTSEEPEGLKTMVTVFMTNG
                     QTLDIEAVRVSQNQNQQCVQIRGAGMRSADRSALNTAYSMGASGADVVRLQNEVNTLR
                     SNRESEVKNAVLEALRRYRYFIYTRYNWTTGKGFSGKNMISDVYDDGRFTYIRIAKPN
                     RGLLAVHADVGGKPAVIPTKYDDAYAIYHMSGIYPKFTLTLDGVKLEVSRADNATDGE
                     S"
     gene            7243..8649
                     /locus_tag="ACHK1J_003398"
     CDS             7243..8649
                     /locus_tag="ACHK1J_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO7912629.1"
                     /translation="MEERQEKVEEKPATYRKKIQYYAFGTFGVILVILFINMVISIAS
                     RNEEEPKENAKQETASTAPNTTEKFNALLKNSGARPSASVNQANTVGNKDSASNGNPN
                     SETDKKPVHPYSREKEASQERGARASDAGSPDEIRARFKADEINRALRARSDKTNFDS
                     TKNTVAGSGNAYAPAASFTGNRTNAQRISDINAEQNGLQERIQMMESKVRADEAALPG
                     RLEEARIRGEQQFAEARQGGAGSSATSVTKGSTQSVPSGVVGYTASNPYGASVDGMMK
                     LPVGTVLNAITTFTAISDYSGGSMKAMLTHDIYDATHSYILAPKGSEFVIRVVKASQV
                     NEILQSRIGFQVTWLVLPDGNRVDFKTSSSLDRMGTPAVEGDEVDRHLLAQFLGVAAY
                     ALVGTKTSYEGSGDSNESFAGNFGEGARTQAGNIAQKYLQVVPTTTLHAGAPIRVFTE
                     DEIYMKPWRNIYETDYTG"
     gene            8720..9121
                     /locus_tag="ACHK1J_003399"
     CDS             8720..9121
                     /locus_tag="ACHK1J_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912630.1"
                     /translation="MRESAPVQISQQDVHVQGNFQELQPLDRNQKDPRWQYIDPLSVY
                     MRTVWPDNIRTIRAAVNYLLEPTGYRLVTSHPAPREASLLVDKPLPPIARMQRTMPVV
                     DALQLLVGLDNYVVIDRAHRLVSFQKNGGGV"
     gene            9133..9342
                     /locus_tag="ACHK1J_003400"
     CDS             9133..9342
                     /locus_tag="ACHK1J_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912631.1"
                     /translation="MRIIFVAFLAIATVILCLFMTGCAQRYESGHDQLFELSERHIIY
                     RDLTVPASMSNLPEKQQISVPGIEP"
     gene            9360..10016
                     /locus_tag="ACHK1J_003401"
     CDS             9360..10016
                     /locus_tag="ACHK1J_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO7912632.1"
                     /translation="MLAFLISGGISASTDEEILLASITAPGVTSFTDESPSSEWITAM
                     QGRWGSTTDMKDMRSLREIREAVIAEINAVSEPVVEKTGPENKESTTGIKISEKIQEI
                     INKYAEKYQISETLLKALIKTESGFNPNAVSRKGAKGLMQLMPIHTKRHNADPFDVET
                     NVKIGTGHLSYLLDKYGDLRLALAAYNAGEGAVDKYGGIPPYKETQQYVNKIMAMLGG
                     "
     gene            10019..10378
                     /locus_tag="ACHK1J_003402"
     CDS             10019..10378
                     /locus_tag="ACHK1J_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO7912633.1"
                     /translation="MTKIKILILAAFVSFGTVHSASAENGSDALSLTGEFLEQPVNSS
                     DPCTVFLCMAGMLKGESPSECSGAKKKFFSIIKRKHGSFHPGRTFNARKAFLGDCPSA
                     PGDIVGKILDKYGKLRW"
     gene            10394..10837
                     /gene="ssb"
                     /locus_tag="ACHK1J_003403"
     CDS             10394..10837
                     /gene="ssb"
                     /locus_tag="ACHK1J_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7912634.1"
                     /translation="MARKGVNEVTLVGFVGNAPVISQASSGDKIAVISVATTETWTDR
                     KTGAEKEKTEWHRVVIFGKTAEITEQYVKKGTLLYIRGKLQTRKWLDRDNMERYSTEI
                     IVNPAGGEMQMLGSRQDKVTGGKAGNDIPENGAGSTDKQQSNETD"
     gene            11068..12051
                     /locus_tag="ACHK1J_003404"
     CDS             11068..12051
                     /locus_tag="ACHK1J_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO7912635.1"
                     /translation="MSPAIDYFAANLPYRPYHANELLYGLRIDKAAKAVLARYIQHNP
                     PHAMYWLVFDVDRPVAAIDWNDLNAPTPNFTVKNPVNGHAHLFYGLKTAVRVAKDGSI
                     KAIEFAAAVERGLRDKLKSDTGYAGLICKNPLHTYWQVVEWRGELYELAELADYVDLY
                     EPTAANDEQAFDIDFSYGLGRNCWLFNLTRIWSYTAIRQGWPDFPQWKEAVIQRVEMY
                     NVQLSVPLSHKECVCIGSSIAKWTHKRFTEAGFEQYVADTHTSEIQARRGRKNRSEVQ
                     AVKGRKGGIAKGNAYGDKREQARVLRVKGLSYQAIADAIQVSRRSVINWCK"
     gene            12288..12554
                     /locus_tag="ACHK1J_003405"
     CDS             12288..12554
                     /locus_tag="ACHK1J_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO7912636.1"
                     /translation="MMMVCILVVSNNGREELIDFNPDHDLAHIIKSYRTPENRMVCII
                     QDGKRILRWDRSYASRAKNHWRKVDPDSFEILGSVEYLRYVGQG"
     gene            13057..13770
                     /locus_tag="ACHK1J_003406"
     CDS             13057..13770
                     /locus_tag="ACHK1J_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="HFO7912637.1"
                     /translation="MAEKNNSLIPRISPSGVAKQSPCYNVVSRKRRRFLNRWIKSLAT
                     VAGRVKIKNELCRRIRHNRYWVNEANKFGIEALCELMLAILDDLDLRSWETRHNLETL
                     AERAGLVTRSDAGRKSISRASRGCDRLVWLNAIITDKAPFNPYDARCACKHIEVTEDF
                     FAILGIPLKLVYRERARLLKADPNEIIYSGDARLIAIRVQNWTRKAAAGLARMKAKRD
                     AARQRKKEYYAHPPTPIPL"
     gene            14461..14769
                     /locus_tag="ACHK1J_003407"
     CDS             14461..14769
                     /locus_tag="ACHK1J_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="HFO7912638.1"
                     /translation="METFDTDKFRSELDLLSKRIMPGCGLVFELYQRRLSAAIDEFIA
                     RLPKEQHAQAFELARQEFDYLSAEEIADEIRRDAEKGYCCHGFDRDCCPLGCGDLDDY
                     "
     gene            14835..15485
                     /locus_tag="ACHK1J_003408"
     CDS             14835..15485
                     /locus_tag="ACHK1J_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912639.1"
                     /translation="MMVNTKKFVMIALSSLLLAGCDSAKDANKSNFEKAINTKLEKEC
                     ITISPLGRFDSKPYPVQLSVAQTNRYTSQERADEINTKEFSMFDALVKAGLLTVKDTL
                     VDETIGFSKTGKKVPGREYALTENGKKYLKSPERPDFCIGHYKVDEIIDFTEPGDAMG
                     MKITRVNYTFSPTNIAEWAKSDDIQAAFPLLKFNLKEKQKNRLSLVLKNDGWSAER"
     gene            15696..16181
                     /locus_tag="ACHK1J_003409"
     CDS             15696..16181
                     /locus_tag="ACHK1J_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912640.1"
                     /translation="MNNLRDKKSNPVITLIAVVISLFFLPAIAAGLGTMGEIYIDGLR
                     ELPSFGIKAISVAIWMLLVAYLMCCFMKAKKDFIRLPAILTFIGTVLLPFVSSFQKMM
                     IISGSDVSTKATFLSKVTEGLGTMKSGFMFIVPALLCAIASIMLAVGMINILRNRYMG
                     R"
BASE COUNT         4792 a         3081 c         4058 g         4609 t
ORIGIN      
        1 aatatactac gtcattcggt aagaaatcaa atggctgaaa taaaaggaaa aataaccaac
       61 ccagcactgg ttaatgcaaa agttttttac aacagacgaa caaagctggg gatacctaca
      121 gaggtctttt tgatcctgat gttcctgaat gtttttgctg ttgtgctgtg tgttatgtat
      181 ttcagcacat tttccgttat ttcgctgatt ttatttgttc tggtatcggt tgttcctatg
      241 aagttcgttt ttcatggcga tcccgatgct cataaagcct ggatttatgg ttatttgtca
      301 ccttccaggc tgaacaatac ccacgcagtg aagcgtcgta tcacatttat agacgctatt
      361 tagggattta accagtaaaa gaggtgtata caatgctcaa tcgcatttcc agaaagatat
      421 caacattcca gataatgttt tacgtagcat tagtgacagg aagtgtcaca ccggctatgg
      481 ctgctggtaa tagtacagtt aaacttccta cgccggatga tagtttttta cagaaaatcg
      541 ttaactggat gcaaagtatt ctggatctgg ttggtggggc tggagctttg ttctttgcgt
      601 ttgtcagtgg gtttgcttgt attgtgttgt ggggacttgc accgcaacag agccaggcta
      661 tcgggaaact gtttcgtgct gctgttgcag caattgcagc gtttaacctg gcgttcttta
      721 ttgcctggct gcaaaaatga ggtgatccac gatggggcag agctataacg aacatctgaa
      781 tctggcaggc atcgaacaac attccgggca ggtgtatacc tatactggag gtgcttttgg
      841 tgctattgaa ctgacaggtg ttgatccaac gggtgttacc ccttctcagg gtagccgtat
      901 ttctttatta cttcgtaata tcgttcagtt actgcccctt tctgtcacac ttactcagta
      961 ttatatccat aatgacggcg ttaagatccg gctgacaggg cgtgataatc aacgcagtca
     1021 gttactttct atgcgtcgcc aggcatatct taataaatgt cgtaatttaa atgcctcaag
     1081 aattatatgg acgctggatg ttcacccgga agagaatatt aatcgcctgg catctgccga
     1141 gtttttaaag aatcttttta acagcatgtt tgatgacaca gccagacgta aagtcaggct
     1201 ggcactgtca aatcgcgaat cgtttatcat tgagcgtcag gcatttgaaa aacagtgcct
     1261 gttgctgaaa gaaacattac gcgatctgga cttacgcctt tcttttattt cagaagataa
     1321 caggattctt gattcagaag ggatctggcg attacagaag ttccttgcaa catttaatca
     1381 ccattggctg gctccacgcc gtaaggtcac tgtacccaat gatatgtggg attgtctggc
     1441 gcttgaaggt gacgatatcg aggccattac ctggcgtggc gttgatatgt tgaagatcca
     1501 gggggtacgg cctgtttatg ttcgcctggc cagtgtgttg aaaattggcg atgaatctgt
     1561 acctgaatgt gcctgggcgc tgacaaatgg cagaccagtt ttacagcggg gtaactacgt
     1621 ttacttcatg cggttcactc ccgccagtgc ctttaagaag tcactgatga tcagcgggaa
     1681 agaaaacgaa cttgtcagga ctcaaatcag tttgagcgat ttaatgactg acagtacgag
     1741 caaagatatt ctcgatgaaa aattgcgcag aaattcacat ctgatgaaga tgcgcgaaga
     1801 actggagaat atttcatatt cagatgagcg cctggggatc tgccaggccg ggattgctgt
     1861 atttaataca gatcccgata aattacttga gacttgtgcg gatattaacc gtagtgtctc
     1921 tcagaccatg caagttgtat gggagacggt agcacaactg gatgcttaca aggttatgca
     1981 accagcctac tcacgcaata catttcgtgc attacagctt aacgcatctc agatgggggc
     2041 tgcatccttg ttctttaaat cccatccagg tattcctgag tgggagctaa atggacgcat
     2101 ggaggaagcc ttctatattc ttgaatcaga tgatggcgtg ccgtttttct ttacgccaaa
     2161 agttaaagaa aagctactgc ttcttggtaa tggggcgacc cgtggcggca agacttttat
     2221 gaaaaactgt attgccagcc attttattaa attcggtggt ctgtataact gtctggatat
     2281 cgatcctggc tcaatcccaa tggctaattt ctttggggat gatggggctg catttaccct
     2341 tgatagcgat tttcagtgtg gttttaacat gttcagtatg gcgcaaagca aggatgacag
     2401 ggtatttctt gcgcatctta tagagcagtt aaaagcaatg gcagcagaaa atgacagtga
     2461 gcaggataaa tatttaacgc ctgatgaact tgaaaatata acagtgtgtg cgagtgatct
     2521 tcttgtacaa aaattttcgc cgaatgaggg gcgtttaagc tcaatcacgt tgtcaacgct
     2581 ggtttctaag tgtggtcaga gcgttaaacg caagttagcg ccatttatta gtaatggtat
     2641 ttatggacgt ttatttgata acaaagagga cgcgattggt gttcttgata agccggtagc
     2701 tgtttacaac ctttcaggtg tgaaagataa ccataatctt gcgcggcttg tgcaccgtga
     2761 aatcttcttt cgtgtcgttc gtttgtttga atcaccggaa tatcgcacga taccaaagtt
     2821 ttttgaaata gatgaggcac agtacaccct gtctgtacca catgccgcag agttcgctac
     2881 acagaaagcc cgaacctggt ttaagcatgg tgggggtatg ggattctgga ctcagaaccc
     2941 tgagcactat tcatcactgg atgagtggac tacgttacgt tcctcggcgt cggtgtttat
     3001 ctttatgccg gataatgagg caacagttaa agcattccat gatgcttatg gcttcgatga
     3061 agaagaagtg aatattattc gcagccttaa ggtaaaacag cagtcgtata taaagatccc
     3121 ggacatgcag attgcaaaag tggtgaatct gtttgttgag gctgaacagt atgctgtttg
     3181 tacatcaacg tctaatgaag cagccctggc agcggaaata tggaaacgtg aatcagatcc
     3241 cgatgttgca gtgcgacaga ttgtagaagg gctgataacg aagtgtaata aggacttgat
     3301 cccaacgcct acagagactg aaaaggataa aacggagggg tttttcttat gagaaaacaa
     3361 cttgcagtag tcttgttttc tgtaatgaca ggattgatta caacacctgc tatttcttct
     3421 ggcattcctg ttgctgatgc tgctcagatt cttggtcagg ccagggctat gatgcaggat
     3481 atcaaaaact ataaagaata tatggaacag acaattctca ataataaaga attgctggaa
     3541 gcatataagc aatacaacca gatgcttgag gattataagc aaattttacg tgaggccgaa
     3601 gggctgaaag ataaactttc atcgattgac tataagaatt ttatggatga aatagccctt
     3661 atctctaacc agttcgatcc cgtttatggt ggggggcgta tggttaaaaa tacgggtaat
     3721 gcgccgtggg ataatgccta tgagcgtaat ttgttgctta atggctatgg gcttacaaac
     3781 gaagaatatg ccgaaatgct ggcagcgata ccatatacga gtgatgaccg cgaacgtgcc
     3841 cggcagatgt ttgaatatag gcagcgcagg gttgttgaag ggatttcaaa agatgccttt
     3901 gttggtgaat tgcaaataca attaaaaaag caaggaaaaa ctctagggga attacaggaa
     3961 agacgtttaa ggttaggtga taacgatacg acagcctcta tccagttttt agctgaacaa
     4021 aatgaacaac tgttacaaca aattcagagt ttacagttac agcaagcaga ggcgatgaaa
     4081 tatagtggtc gtcttgagga tcactatttt aacaagcgag cgcaacagga agaattaaag
     4141 gcacgtcagt tacagagggc atggcaatga aaaaatatct gattcttttc ttcatgatgt
     4201 tttcagcctc ggcaatggct aagattggct atgttgatga gcatcaaaag gaagttgacc
     4261 taaaaattga cgctctgatt tctaaatatg aaaaagaatg tgaaggtaaa cgcaatagta
     4321 atatgtgtaa atcccaggca tgggataagg cccattttga atatgaagat gaatttagag
     4381 gcgaagataa atataatcat aagcattatg atggcctgac aaaagatcag gcagtcgcga
     4441 agttacatga acttattaaa ttgcataata ttgtttctaa agatgaacgt aatcctgaaa
     4501 gttggcctgg aaaattagac acactaacta ttaatggtga gattaactat atagttagaa
     4561 aacactggcc agcatggatt aatccatgtg ataagatttg tgcggaactg ttacttagac
     4621 aaataggtaa atgatgtagc atttaacaag ctatatcttt aatttggggg ctattatgct
     4681 tcgaggattc tttttgtctt taattaacag actcttattt gtagtcctgt ttttatctgt
     4741 ctcattttat gccagtgcag aaacagctat gcgcacaaac tttcagaatg gcgtaagtga
     4801 gatggttaat aaaatactaa atagcccgga tagtgattta cgtgtctgga ctattgccgc
     4861 atcaactttc ttggctgcgg taacggcatt cgtctatttc tggcggttcg tggaaaatgg
     4921 tggggcgtta gttgatttat tcacgcttgc aatcatgttt atgttatcaa tgtcattgct
     4981 tgcttcttac tcaatggttg ttgatactat cacaggcgct ttttatgcga tatcaaatac
     5041 actacagaaa ctggcgttag gtaatattaa gccgagtttt ctgcaaaatt ttgtagaggg
     5101 cgtgttaaca aaggctattt cagcaccatc ggttgatatt tttgatggca tgaatatggt
     5161 tggcatggcg ctcttgtggg aaatatgttc tgctattttg tctgttgcta tatatcttgc
     5221 tgatgtttgg attgatgttg ggacagcaat agcaaaaatt acaggtgtta tgtttattcc
     5281 ttttttggtt gcgccagcaa caagaggaat ttttgatgga tggtttaaat tctatattgg
     5341 atgggggctt gcagcggttg tacttaaaat aacatctatt gtggtaatga taatgatcag
     5401 ggcatcaatt aactctgcgg gtagtttcca gggcgctggc gatgttatcg cgtcagatgg
     5461 tactgttaat ggcgttctta atataacagc taataatatc agcttgttac gtgatatttg
     5521 tgcatattct gtaatcgctg caattatggt gttcagttgt tttatatttg cgtacatgat
     5581 tggttctggt gtcggctctg caagtcaggg gttgaataat atggcagtaa aactagccaa
     5641 gaaagcggct aaagcattat tgtaaggtga acgtatgctg cttgaaaaaa aagaagtcga
     5701 aaaagtaaat cgcacagcct ttaaggatcg tgttggtgaa ctaaaaaaag acagaaattt
     5761 gtttttctgg attgccttgc ttcttgccgg gacgacgata tatcagtcat attctgttca
     5821 ggaggcaata gagaaaagcc agaatactac agaagtagta tgggtgaaat tattgcctga
     5881 tggttcacat aaaatatcac aatttacgcc agacaatgag cagcctatat atacaccaac
     5941 agtgaactca ttgctgtcaa agtatgtgca gaagcgctat ggacagttaa aagagactat
     6001 agaacgggat tatgcagaag cggcggtatt tatgtctcct gttcttacag gggagtttgc
     6061 tgatcccaaa gggtttaatg tcgctcagaa agttgtggat attcaggccg atatggcagc
     6121 aaaaaggatt tatatagaaa taaaagatat tgaccattat gacgcgatag aaggtcagtt
     6181 tgatggtgat gttaaacctg taattcgaac gcttgttact tatcaggaga tcacaaggga
     6241 ttatcagggt aaagaaattt catcggttca acaaatgagg cgtcttcaat ggacgttgtt
     6301 acaacgtaac gaacttagta aacaatcgct ggactggctt aacatcaatc cgctgggaat
     6361 ccagattatt gatgataaat ccgtacaaca gtaaaggaac ttaatcatgt tagcgaaaag
     6421 aactctctta ttttctggag tcctggctgt tgtcatgact acgttcagtg caacagcaca
     6481 gcaatgtagg gtggttaact ggcggcctgg aacaatagtt aaagtgcagt ccgctttata
     6541 tctgggaact cgtatagaac tgccggaagg ctcaatgtta ttaacgaaag aacccttttc
     6601 ttctaatgcg ctatgggcca gcacgggagc cgcaaatcag gtcatgattc agcctaccag
     6661 cgaagagccg gaagggctga aaacgatggt aacagtattt atgactaacg ggcagacgct
     6721 ggatattgag gctgttcggg tatctcagaa ccagaatcaa cagtgtgttc agatccgtgg
     6781 tgcaggtatg cgttccgcag acagaagtgc attaaacacg gcttacagta tgggggcatc
     6841 cggggctgat gtagttcgtt tacagaatga agtgaataca ttgcggagta acagagaaag
     6901 cgaagtaaaa aacgcagttc ttgaagctct tcgtcgatac cgatatttca tttatacccg
     6961 ttataactgg acgacaggta aaggttttag cggaaagaat atgatctctg atgtttatga
     7021 tgatgggcgt tttacttata tccgtatcgc aaaaccgaat cgtggattac ttgcggtaca
     7081 tgcagatgtc ggaggaaaac cagcggttat tcctaccaag tacgacgatg catatgccat
     7141 ttatcatatg tcagggatat atcccaaatt tacccttacc ctggatggcg taaagctgga
     7201 agtcagccgt gcggataatg cgactgatgg ggagagttaa ttatggaaga acgtcaggag
     7261 aaagtagaag aaaaaccagc tacttaccgt aaaaaaatac agtattacgc ttttggtact
     7321 tttggtgtga tcctggtaat tcttttcatc aatatggtga taagtattgc ctcaaggaac
     7381 gaggaagaac caaaggagaa tgcgaaacag gaaactgcca gcactgcgcc aaatacaacg
     7441 gaaaagttta atgcgctact gaaaaacagt ggtgcacgtc cttctgcctc cgttaatcag
     7501 gctaatacgg ttggaaataa ggatagtgcc agtaatggaa atccgaatag tgaaacagat
     7561 aaaaaacctg ttcaccctta cagccgggaa aaagaggcgt ctcaggagag gggggcgaga
     7621 gcaagtgatg cgggatcacc tgatgaaatc agggcacgct ttaaggctga tgagattaac
     7681 cgagcactgc gtgcaaggtc agataaaaca aattttgaca gcactaaaaa tacggttgcg
     7741 ggtagtggta atgcatatgc cccggcagca tcgtttaccg gaaaccggac taatgcgcag
     7801 cgtatcagcg atataaacgc agagcagaat ggattgcagg aacgtatcca gatgatggaa
     7861 tcaaaagttc gtgctgatga agccgctttg cctggacgac tggaagaagc ccggatacga
     7921 ggggaacagc aatttgcaga agcccgacag ggtggagcag gaagtagcgc gacatcggta
     7981 actaagggga gtacccagag cgtaccgtct ggtgttgtgg gatatacagc cagtaatcct
     8041 tacggggcaa gtgttgatgg aatgatgaag ttgccagtag gtacggtcct gaatgccatt
     8101 accacattta cggctatttc tgattattcc ggcggctcaa tgaaggccat gcttacgcat
     8161 gatatctatg atgcgacgca tagctacatt cttgcgccta aaggaagtga gttcgttatc
     8221 agggtcgtta aagcctccca ggtaaacgaa atccttcaaa gccgtattgg attccaggtt
     8281 acatggcttg tattgcctga tggtaacagg gtcgatttta aaacctcgtc gtctcttgac
     8341 cgaatgggga ctccggcggt tgaaggcgat gaggtggaca ggcatttgct cgctcagttt
     8401 cttggcgtag cggcctatgc ccttgtgggg acgaaaacca gctacgaagg ttcaggtgat
     8461 agtaacgaga gttttgccgg gaattttggc gaaggcgctc gtactcaggc aggaaatatc
     8521 gctcaaaaat acctccaggt tgtgccaaca acaacgttac atgcgggagc accgatcagg
     8581 gtctttaccg aagatgagat atacatgaag ccgtggagaa atatttatga aacggattat
     8641 acgggctgaa atattgtttt ttgctgcatt ctttgcattt tcctgtcagg cagcaagaag
     8701 tcctttccag gtagacagga tgcgagaaag tgcgcctgtt cagatatcac agcaggatgt
     8761 gcatgtgcag ggaaactttc aggagttaca accactggac aggaatcaga aagatcctcg
     8821 ctggcagtat atcgatccgt tgagtgttta tatgcgtaca gtttggcccg ataacatcag
     8881 gactatcaga gcagcggtga actatctcct ggaaccaact ggatatcgcc tggtaacgag
     8941 tcatccagca ccgcgtgagg cttctctgct tgttgataag ccattaccgc caattgccag
     9001 aatgcaacgc acaatgcccg tagttgatgc tcttcagttg ctggttgggc ttgataatta
     9061 tgttgttatt gatcgtgccc acaggctggt ttcatttcag aaaaatgggg gcggtgtatg
     9121 aaggaatggt gtatgcggat tatttttgtg gctttcctgg ctattgcaac ggtgattctt
     9181 tgcttattta tgacggggtg tgcacaacgt tatgagagtg gtcatgatca gcttttcgaa
     9241 ctgtcagaac gccatatcat ttaccgggat ctgactgttc cggcatcgat gagcaatttg
     9301 ccggagaagc agcagataag cgtaccgggg attgaaccgt gaacgtccgg ttaattttca
     9361 tgctggcgtt tctgatatct ggcggtatca gcgcatcaac ggatgaagaa attctactgg
     9421 cgtcgataac cgcgccagga gttaccagct ttaccgatga atcaccttct tcggaatgga
     9481 ttacagcgat gcagggacgt tggggtagta caactgatat gaaggatatg cgtagtttgc
     9541 gcgagataag agaagcggtg attgctgaaa ttaatgccgt gtcagagccg gtggtggaga
     9601 aaacagggcc agaaaacaaa gaatcgacca caggaataaa aatatctgaa aaaatacagg
     9661 agataatcaa taaatacgca gaaaaatatc aaattagtga aactttgttg aaagcgttaa
     9721 ttaaaacaga gtctggattt aatccaaacg ctgtcagcag aaaaggtgca aaggggctga
     9781 tgcagttaat gcctatacat accaaaaggc ataatgctga tccctttgat gttgaaacca
     9841 atgtgaaaat tggtacaggg catctttcat atcttctcga taaatacggg gatttacggc
     9901 ttgctctggc tgcatataac gctggggaag gtgctgttga taaatatggc ggtatccccc
     9961 cttataagga aacacaacag tatgtaaata agatcatggc aatgttagga ggttaaatat
    10021 gacaaaaata aagatactga tacttgcagc ttttgtctca tttggaaccg tgcatagcgc
    10081 cagcgcggaa aatggaagtg atgctctgtc actgacagga gaatttcttg agcaacctgt
    10141 taacagtagt gatccatgta ctgtttttct ctgtatggct ggaatgctta agggggaatc
    10201 gcctagtgaa tgtagtgggg caaagaaaaa atttttcagc attatcaaaa ggaagcatgg
    10261 cagttttcat ccaggaagaa catttaatgc ccggaaagcg tttcttggtg attgtccgtc
    10321 agcacctgga gatattgtag gcaagatact tgataaatac ggaaaattac gctggtaaca
    10381 gaaggggtaa acagtggcaa gaaaaggtgt aaatgaagtc accctagttg gttttgttgg
    10441 taatgctcca gtgatatctc aggccagtag cggggataag atcgcggtta tttccgttgc
    10501 aacaactgaa acatggacgg acagaaagac aggtgctgaa aaagagaaaa ccgagtggca
    10561 tcgagttgtt attttcggaa aaacagccga gataacggaa caatatgtaa aaaaaggcac
    10621 attgctgtac attcgcggga aactgcaaac ccgtaagtgg ctggaccgag acaatatgga
    10681 gcgttacagc acggaaatta ttgttaatcc ggcaggggga gaaatgcaga tgctggggag
    10741 tcgccaggat aaagtaaccg gaggaaaagc cggaaacgac attcctgaaa atggcgctgg
    10801 cagtacggac aagcagcaaa gcaatgaaac cgactaacgc gccttcggct tgttcaaccc
    10861 cggagtcact cagaagcaag ttccgagtga ccccggcgtt tttggttttt actacgggga
    10921 tgataatacc catgagattg atacttggca gagtcagctt gtgggcatta tcaggggact
    10981 ataaaagccc atttaaattg tatttaaatc atccggtaac ggcaagaaaa ataatgtgtc
    11041 attcaggcat aagtgcaggg ccatatgatt agtccggcaa ttgattattt tgcggcaaac
    11101 ttgccatacc gaccttatca cgcgaatgaa ttgctctatg ggcttcgtat cgataaggca
    11161 gctaaagcag tgctggcgcg ttatatacag cataatccgc ctcatgcaat gtactggctt
    11221 gtttttgacg ttgacaggcc agttgcggcg attgactgga atgatcttaa tgcgcccaca
    11281 cctaatttta cggtcaaaaa tccggttaat ggtcatgcac acctgtttta tgggttaaaa
    11341 acggcggttc gtgttgccaa agacggtagc attaaggcga tcgagtttgc tgctgcggta
    11401 gagcgaggat tacgtgacaa acttaaatca gataccggct atgccgggct gatttgtaaa
    11461 aatcctctcc atacatactg gcaggttgtt gaatggcgtg gagaacttta tgagttagct
    11521 gaactggctg attatgttga tttgtacgag ccaacagcgg ctaacgatga acaggcattc
    11581 gatatcgact ttagttatgg gcttgggcga aattgttggc tcttcaatct gacccgtatc
    11641 tggtcatata cagcaatccg gcagggctgg cctgatttcc cgcaatggaa agaagcggtg
    11701 attcagcgtg tcgaaatgta taacgtgcag ttaagcgtgc ctttgtctca caaagaatgt
    11761 gtatgcattg gctccagcat cgccaaatgg acgcataaac gcttcactga agctggattt
    11821 gagcaatatg tggctgatac ccatacatca gaaattcagg ctcgtagagg gcgtaagaat
    11881 cgctcagaag tgcaggctgt taaggggcgt aaaggtggca ttgcgaaggg gaatgcctat
    11941 ggtgataaac gcgaacaagc aagagtatta cgggtaaaag gattgagtta tcaggcaatc
    12001 gctgacgcaa ttcaagtatc tcgacgctcg gttattaact ggtgcaaata gcaacctata
    12061 tcagataaca gcaccggatc ttgatccttg cagacagatc ctttgccttt aataatcttc
    12121 cgattttcag gtattgagtc ttttatgagt gagatgatag cgcccgccgc agccccagcg
    12181 accgaaccgc caggtgagcg agtgggcgag gaggcgcaag ggaggcagag gttgatgttt
    12241 gcacttacgc tcgatgtttg agcagcgcaa gaagacaaat ggagagtatg atgatggtct
    12301 gtatacttgt tgtgtcaaat aacggtcggg aagagttgat tgattttaat cccgatcatg
    12361 atttagcgca cattatcaaa tcataccgga caccagaaaa caggatggta tgtattatcc
    12421 aggatggtaa gcgcattctt cgttgggatc gcagttatgc ttcccgtgca aaaaatcact
    12481 ggcgtaaggt agatccagac agttttgaga ttttagggtc tgttgaatat ctccgctatg
    12541 ttggtcaggg ttagttgggg cgttgaaaac ggaaaatacc ccacgctaag actgtttttt
    12601 aaccaggcta tgataatgct atctttgacg gatgatggtc attgctcttt tgttaatgct
    12661 aacggacgcg tacattagca ttaacttttg tgttatgttg cacgtgaatt tttgagttat
    12721 aatatttata taatgttatg aatattaaag ataaatgatg aacatgcttt gtgatggctt
    12781 ttggcgcata gattgatctt aacatctgtg catgttattt ttatgcggtt ttgacgaatt
    12841 tataagggtg ggacgttccc gtaacagcgt ccaagggagg gggatagcga atttatagcc
    12901 cccggaatgc aacaaccccc tgatatctct cttagagctt ttgccgggcc aaagaagata
    12961 atcagggggt ccaaagcagg ctgggtgcct gtaagggatt atagcaaatg catttctcaa
    13021 tacaagacct ctgtccggca ataagtgcgg gcggtggtgg ctgaaaagaa caactcctta
    13081 atcccccgca tatcgccgtc aggggtggca aaacaaagtc catgctataa cgttgtgtcg
    13141 cgtaagcgac gccgtttcct caatcgctgg attaaatcac tggcgacggt cgcagggcgt
    13201 gtcaaaatca aaaatgaact ctgccgccgt atccggcata accgatattg ggttaatgag
    13261 gctaacaagt ttggcataga ggcactttgc gaactgatgt tagctatcct ggatgatctc
    13321 gatttacgct cctgggaaac caggcataac ctcgaaacac tggcagaacg tgccggactg
    13381 gtgacacgca gtgacgcggg gcgtaaatct atttcccgtg ccagtagggg atgtgatcgc
    13441 ctggtctggt tgaatgcgat cattacagat aaagcgccgt ttaaccccta tgatgcacgg
    13501 tgtgcctgta aacacattga agtaaccgaa gacttctttg ccatactcgg cattccgtta
    13561 aaactggttt atcgggaacg tgccaggctg ttaaaggcag atcctaatga aattatttac
    13621 tccggtgatg ccagactgat agctatcaga gtccagaact ggacacggaa agcggcagcc
    13681 ggattagccc ggatgaaagc gaaacgcgac gcagctcgac aacgcaaaaa agaatattac
    13741 gctcacccac ctacccctat tccgctctga tacaggctaa cttaccgcct tatggggcgg
    13801 tgtatcggca cgtccataat aaaaaactgt acaaaaaata gtcttagcgt gggttatttt
    13861 ccgttttcca tgtacagaaa agagcaggta taagctgtat cattgggtaa actatgcaca
    13921 ggttgtggat aatcatactt aaagtgcaaa cgatatgccg caattactct tttgcttctc
    13981 ttttagttac ttacgtacta aaagagaagc aaaaaagaaa aacattactg ttatcccctt
    14041 taacaagtga ataacctaaa aacatcggcg caagcgcctc gaaagtagca cccctcgcaa
    14101 gctcggggca ttagaaacaa cagtatccca tcatcacaat cgaactatta ttcacctatc
    14161 tttgctgtaa catcgacggt ctacgaccac ctaccacctg aatagctgta agttccgtgc
    14221 ggtcaggctt agcagggggc tgacgcggtt cctggcgaca tgaaactccc tggttagggg
    14281 tatcggcgcg acctggcatc tgtgactcta tgaaaccatt tttcgcgcct tccatgccgc
    14341 taaagcggca tattccataa gaatttacat gcgcgtatac tttataatca gtatcaacga
    14401 aagttgcttg cccaaatcag aggcaacaat cgcatcaaat aacttgcgca ggtgataatt
    14461 atggagactt tcgatactga taaatttcgc tcagaactgg atcttttatc aaaaaggatc
    14521 atgcctggtt gtggtcttgt ttttgagcta tatcaacgtc gtttgagcgc tgcgattgat
    14581 gaatttatcg ccagattgcc gaaagaacaa catgctcagg cgtttgaact ggctcgccag
    14641 gagtttgatt atctttctgc agaagagatt gcagatgaaa tacgccgaga cgcagaaaaa
    14701 ggctattgtt gccacggctt tgatcgcgat tgctgccctc ttggttgcgg tgatttagat
    14761 gattattaat ttaaagggat aaatatattt acatactgaa aatataggct gattttttct
    14821 gataaagagg aaaaatgatg gttaatacaa aaaaatttgt gatgattgcg ttaagttcat
    14881 tattgttggc agggtgtgac tcagcgaagg atgccaataa atcaaacttt gaaaaagcga
    14941 ttaatacgaa acttgagaag gaatgcatta caatcagtcc tcttgggagg tttgatagta
    15001 agccttatcc agttcaatta tctgtcgccc aaactaatcg atatacatct caggaacggg
    15061 cagacgaaat aaataccaaa gagttttcca tgtttgatgc actggttaaa gctggtttat
    15121 tgacagtaaa agataccctg gttgatgaaa ctattggatt ttctaaaaca ggtaaaaaag
    15181 tgcctgggcg tgaatatgct ctgacagaga atggaaaaaa atatctgaaa tctccagagc
    15241 gccctgattt ctgtattggt cattataagg ttgatgagat cattgatttt actgaacctg
    15301 gtgatgctat gggaatgaaa ataacaaggg tcaattacac attcagtcct acgaatattg
    15361 ctgaatgggc aaagagcgat gatattcagg cagcatttcc acttcttaag tttaatttga
    15421 aagagaaaca gaaaaaccgt ctatctctgg ttttgaaaaa tgatggatgg tccgctgaac
    15481 gataggtaga taaatgacgt tgctacgtgg tctccctctt ttgctgattt cacgtagcac
    15541 tctcactctg ccgctacttc tcgttgtgct gaacagatga ttgaaaaata aggagaatta
    15601 aataatctct tgattttata atcctatgat tataagatta ttgtgtatga gtgtttggat
    15661 agagaattat tgtaatcaag tgggagatat tatttatgaa taatttacgt gataaaaaat
    15721 caaatcctgt tattacatta attgctgtcg tgatttcttt attcttcctt cctgcaatcg
    15781 ccgcaggact ggggactatg ggagaaattt acattgatgg tctgcgtgaa ttaccgtctt
    15841 ttggtataaa ggcgataagt gttgcaatct ggatgctttt agttgcatat ctgatgtgtt
    15901 gcttcatgaa agctaagaaa gattttattc gcttacctgc tatactgacg tttattggga
    15961 cggttctcct gccttttgta agtagttttc aaaagatgat gattatatcc ggttcagatg
    16021 tttccaccaa agcgaccttt ttgagcaagg ttacagaagg tctgggaaca atgaaaagtg
    16081 gatttatgtt tatagtacct gctttattat gtgcaatagc ttccattatg ctggctgttg
    16141 gaatgataaa tattttacgt aatcgttata tggggcgcta atctgatgtg cggtgattgt
    16201 gaggttgatt tgattataga agggagttaa tgtggaactg ataacagata atttttatga
    16261 gtgggtggtg gtgtctctcg ctattttgaa ttttatcgtt atattgaaat ataacgataa
    16321 aataactttg ttgttaacga cgatgattag cctacttctt gcttcaatta tatttgccag
    16381 ataaacgact taatagctac aggggggggc aagccggaaa cctggatttt tccgcgatca
    16441 gtaaggggtc ggttagggaa acgtacaata ttgtacgttt ccctaacgca gccctacagg
    16501 aactggcacg gctgaaaatc tgaccgcctg gcctgcatac
//
LOCUS       DAZGBC010000110        16358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGBC010000110 DAZGBC010000000
VERSION     DAZGBC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.111"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..153
                     /locus_tag="ACHK1J_003410"
     CDS             <1..153
                     /locus_tag="ACHK1J_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912641.1"
                     /translation="TYKTVLLDFNNLSNEEANQLRISSLYRDMVRYAQYRFSKVLSEW
                     TPDAWL"
     gene            437..2587
                     /gene="dinG"
                     /locus_tag="ACHK1J_003411"
     CDS             437..2587
                     /gene="dinG"
                     /locus_tag="ACHK1J_003411"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO7912642.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2615..3577
                     /gene="ybiB"
                     /locus_tag="ACHK1J_003412"
     CDS             2615..3577
                     /gene="ybiB"
                     /locus_tag="ACHK1J_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO7912643.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3718..4803
                     /gene="hcxB"
                     /locus_tag="ACHK1J_003413"
     CDS             3718..4803
                     /gene="hcxB"
                     /locus_tag="ACHK1J_003413"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO7912644.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDGGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            4870..4929
                     /gene="ybiE"
                     /locus_tag="ACHK1J_003414"
     CDS             4870..4929
                     /gene="ybiE"
                     /locus_tag="ACHK1J_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO7912645.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5032..5292)
                     /gene="ybiJ"
                     /locus_tag="ACHK1J_003415"
     CDS             complement(5032..5292)
                     /gene="ybiJ"
                     /locus_tag="ACHK1J_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO7912646.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5557..5823)
                     /gene="ybiI"
                     /locus_tag="ACHK1J_003416"
     CDS             complement(5557..5823)
                     /gene="ybiI"
                     /locus_tag="ACHK1J_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO7912647.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(5897..6574)
                     /gene="ybiX"
                     /locus_tag="ACHK1J_003417"
     CDS             complement(5897..6574)
                     /gene="ybiX"
                     /locus_tag="ACHK1J_003417"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO7912648.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6616..8898)
                     /gene="fiu"
                     /locus_tag="ACHK1J_003418"
     CDS             complement(6616..8898)
                     /gene="fiu"
                     /locus_tag="ACHK1J_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO7912649.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAARLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9163..9423)
                     /gene="mcbA"
                     /locus_tag="ACHK1J_003419"
     CDS             complement(9163..9423)
                     /gene="mcbA"
                     /locus_tag="ACHK1J_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO7912650.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            9699..10625
                     /gene="rlmF"
                     /locus_tag="ACHK1J_003420"
     CDS             9699..10625
                     /gene="rlmF"
                     /locus_tag="ACHK1J_003420"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO7912651.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10622..12847)
                     /gene="ybiO"
                     /locus_tag="ACHK1J_003421"
     CDS             complement(10622..12847)
                     /gene="ybiO"
                     /locus_tag="ACHK1J_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO7912652.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13108..13830)
                     /gene="glnQ"
                     /locus_tag="ACHK1J_003422"
     CDS             complement(13108..13830)
                     /gene="glnQ"
                     /locus_tag="ACHK1J_003422"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO7912653.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13827..14486)
                     /gene="glnP"
                     /locus_tag="ACHK1J_003423"
     CDS             complement(13827..14486)
                     /gene="glnP"
                     /locus_tag="ACHK1J_003423"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO7912654.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14625..15371)
                     /gene="glnH"
                     /locus_tag="ACHK1J_003424"
     CDS             complement(14625..15371)
                     /gene="glnH"
                     /locus_tag="ACHK1J_003424"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO7912655.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15775..16278)
                     /gene="dps"
                     /locus_tag="ACHK1J_003425"
                     /gene_synonym="pexB"
     CDS             complement(15775..16278)
                     /gene="dps"
                     /locus_tag="ACHK1J_003425"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO7912656.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         3932 a         4112 c         4383 g         3931 t
ORIGIN      
        1 acatacaaaa cggtattgct tgatttcaat aatctgtcta atgaagaggc caaccagttg
       61 cgcataagtt cgttatatcg tgatatggtc cgctatgcac aatatcgttt ttctaaagtt
      121 ttatccgagt ggacgccaga cgcatggctg taaagatcgc agcagaataa tggcattgat
      181 ggccctggct caggccaggg tcggcaatac cggttcctgc gaagcgatga cggcggcaag
      241 tgtaaccacg aaatgcggta tcacttgctg ttttctgccc tgaagactgg cgctcactgt
      301 gccgtcgaat tttcaatcaa atatattgcg agagggcgtg tttgcgagcc gctttccaga
      361 aacagaaaaa ccattacccc tgaaaaccga aaaatgccac aatattggct gtttatacag
      421 tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg ccgcctggta
      481 taaggcgctt caggaacaga tccccgactt tattccccgt gcgccgcagc ggcagatgat
      541 cgcggacgtc gccaaaacgc tggccggaga agaagggcgg catctggcga ttgaagcccc
      601 cactggcgtt gggaaaacgc tctcctattt gattcccggc atcgccattg cccgcgaaga
      661 gcaaaaaacg ctggtggtga gcaccgccaa cgtggcattg caggatcaga tctacagcaa
      721 agatttaccg ttgctgaaaa agatcattcc cgatcttaaa ttcactgccg cttttgggcg
      781 tgggcgctac gtttgcccgc gtaatctgac ggcgcttgcc agcactgagc ccacgcaaca
      841 ggatctgctg gcgtttcttg acgacgaact gacgccaaat aatcaggaag agcaaaaacg
      901 ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg gatggtctgc gcgatcatac
      961 ggatatcgct atagatgacg atctctggcg tcgtttaagt accgacaaag ccagctgcct
     1021 caaccgcaac tgttactact atcgcgaatg cccgtttttt gttgctcgtc gggagattca
     1081 ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg aaagcgaagc
     1141 ggtattgcct gacccgaaaa atttactgct ggtgctggac gaaggccatc acctgccaga
     1201 tgtggcgcgg gatgcgctgg agatgagtgc cgaaatcacc gcgccatggt atcggctaca
     1261 gctggacttg ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc cgaagaccat
     1321 cccgccgctg gcgatccctg aacgtttgaa tgcgcattgt gaagagctgt atgagcttat
     1381 agcctcatta aacaacattc tcaatctcta catgcctgct gggcaggaag cagaacaccg
     1441 ttttgcgatg ggcgaactgc ctgatgaagt gctggagatc tgccagcggc tggcaaaact
     1501 caccgagatg ctgcgtggtc tggcggagtt atttcttaac gatttaagtg agaaaaccgg
     1561 cagccatgac attgtacgtc tgcatcggtt gattttgcag atgaaccgcg cgttggggat
     1621 gttcgaggcg caaagcaaac tctggcggct ggcttcgctg gcgcaatctt ccggtgcacc
     1681 ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag ctacacctct ggtttcactg
     1741 cgtgggaata cgtgtcagcg atcagctgga aaggctgctg tggcgcagta ttccgcacat
     1801 tattgtcacc tccgcaacct tgcgttcgct gaacagtttt tcgcgtttgc aggagatgag
     1861 cgggctgaaa gagaaagcgg gcgaccgttt tgtggcgctg gattccccct ttaaccactg
     1921 cgaacagggc aaaattgtta ttccccggat gcgcgttgag ccttccatcg acaacgaaga
     1981 acagcatatt gctgaaatgg cggccttttt ccgtgagcag gtggagagca aaaaacatct
     2041 cggtatgttg gtgctgtttg ccagcgggcg tgcgatgcag cgctttctcg actatgtgac
     2101 ggatttacgt ctgatgttgc tggtgcaggg cgatcagccg cgttaccgtt tagttgaact
     2161 gcaccgcaaa cgcgtcgcca acggtgagcg tagtgtgctg gtgggcttac agtcatttgc
     2221 cgaagggctt gatttgaaag gtgatatgct cagccaggtg catatccaca aaatcgcttt
     2281 tccgcctatc gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa gcctcaaccg
     2341 ctatccattt gaggtgcaaa gcttgccgag cgcctcgttt aacctgattc agcaggttgg
     2401 gcgactgatt cgaagtcacg gttgctgggg cgaagtggtt atctacgaca aacgcttgct
     2461 gaccaaaaat tatggcaagc ggctactgga tgcattaccg gtatttccga tagagcaacc
     2521 ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac gccgtcggcg
     2581 gcgttaatga tgtaagtcag gtaaggagtc gtaaatggac tatcgcaaaa tcattaaaga
     2641 gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc gcggtctgta
     2701 tgcccatatg ctcaacggtg aagtccctga ccttgagttg ggcggtgtat tgattgcgct
     2761 gcgtatcaaa ggggaagggg aagcagagat gctcggcttt tacgaagcca tgcaaaatca
     2821 caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg attgtcatcc ccagttacaa
     2881 cggcgcgcgt aaacaggcca acctgacgcc gttgttggcg attctcctgc acaaactcgg
     2941 ttttcctgtg gtggttcacg gggttagcga agatccaact cgcgtgctga ccgaaaccat
     3001 tttcgaactg atgggtatta cgccaacgtt gcacggcggg caggcgcagg cgaagctcga
     3061 cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc ccgccgctgg aaaaacaact
     3121 ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat accctggcga aactggcgac
     3181 gccatttgcc gaaggtgagg cactgcgtct ttccagcgtt tcgcatccgg aatacatcgg
     3241 gcgagtcgcg aagttcttta gcgatatcgg cgggcgggcg ctactaatgc atggtactga
     3301 aggtgaagtg tatgctaatc cgcagcgctg cccgcagatc aatctcattg accgtgaagg
     3361 gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc gagttattgc cacaagcaaa
     3421 agatccggaa accacggcgc agtggattga gcgttgcctg gctggcagcg aaccgattcc
     3481 cgaatcgctg aaaatccaga tggcttgctg cctggtggct acgggtgaag cggcaactat
     3541 cagcgatggc ctggcgcgcg ttaatcaggc attttaattt ttatctcagc cggatgagcc
     3601 acgtcatccg gcttttaccc caccgcaata tgaaattcct gcatctttat tgaccttctc
     3661 gcgcccggcg tgcagcataa aaaaacaaca aacacataac ataaacagga gttaaccatg
     3721 gaaagtggtc atcgctttga tgctcagacg cttcacagct ttattcaggc tgtatttcgt
     3781 cagatgggta gcgaggaaca agaagcgaaa ttagttgccg atcatttaat cgcggcaaac
     3841 ctggcagggc atgattcaca tggtattggc atgatcccaa gctatgtacg ctcctggagt
     3901 caggggcacc tgcaaattaa ccatcatgcc aaaatcgtta aagaggcggg ggcggcggtc
     3961 acgctcgatg gcgatcgcgc atttggtcag gtcgcggcac atgaagcgat ggcgctgggg
     4021 attgagaaag cgcatcagca cggtattgcc gccgtggcgc tacataactc gcatcatatc
     4081 ggccgtatcg gttactgggc ggagcagtgt gcagcggcgg ggtttgtctc tatccacttt
     4141 gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag ccgctttggc
     4201 accaatccgt tctgtgtggt tttcccacgt aaagatgatt ttccgctgtt gcttgattac
     4261 gccaccagcg ccattgcatt tggcaaaact cgcgtcgcct ggcataaagg cgtccccgtg
     4321 ccgccaggtt gtctgattga cgttaacggc gtgccgacga caaatccggc ggtaatgcag
     4381 gagtcgccgt tgggttcgct gttgaccttt gccgaacata aaggctacgc ccttgcagcg
     4441 atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa cgacgcatca ggaaacgtta
     4501 caaaccagtc ccgatgccat tcttaactgc atgaccacta tcatcatcaa tccggaactg
     4561 ttcggcgcgc cggattgtag cgcgcagacc gaagcctttg ccgagtgggt gaaagcctcg
     4621 ccgcatgatg acgataagcc gattttgctc ccgggcgagt gggaagtgaa cacgcgtcgc
     4681 gaacggcagg agcaggggat tccattggat gggggaagct ggcaggccat ttgtgatgcg
     4741 gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt tctgtcagca gttagccagc
     4801 taaaaaaaag cccgtccagt ggcggacggg caaacaaggg taacatagga tcaatgaggg
     4861 ttagagcata tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg acgtagcgta
     4921 aacgcctgat ccgtccaacg gttcggattt tgtaggcctg ataagacgcg gtagcgtcgc
     4981 atcaggcatc tgttgccgga tgcggcgtaa acgccttatc cgtcctacga attacttata
     5041 aattaccgca gtaccgctta atttattgtt gttggtggcg gaagtgatgc tgtatccact
     5101 cgccccagct gccgccgctt tctcagccag tttcgcttcc agggcatcga gggtggatgc
     5161 gccatcggca gaaaccacgc cgattttatt catgttctgt gcctgggatg ccgttaccgg
     5221 ttccgcggca aacacgccaa atgacagagt tgaaagagcc atagcagcaa caacagtatt
     5281 gatagttttc ataattaatc tctcgcaggt gatttttgta taagtgacgt tgtttcgtcg
     5341 atgtgatgag tatcacgttt ttttacgaga gataaaatcg aagagaattg accgccttgt
     5401 tcaaataaat tgattgatat ctaacatact gataaaccat ttatttgttg tacttatgga
     5461 cttgttttat gcagcgtggc agtcacagca gcgttaaagg cacggaatgc cacgcaaaat
     5521 acaaattata ctcgctatac atctcgccag ttgtagttaa cgtaactggc tgtctttcga
     5581 acctctgcga ttatatcctg tataagctgg tttttgtaaa tctttctcct gctgacaatg
     5641 aatacataag cgcacgccag gaatggcttc ccgacgggct tgcgggatgg gggcaccgca
     5701 ctcttcacat tcatacaggc tttcgccacg cggaatttca ccccgagcgc gggcaatcgc
     5761 atcttcaatt gtactgttga tctgttcgtt gacggcgtca tcgttagccc aaccggatgc
     5821 catatcgtcc tccccatatc aatgcttgta cagttaagtg tagctaatat agggacgaac
     5881 tcgggcagtt caagcgtcag atctccgacc attcccgcag cagattatga taaagattga
     5941 gcagcgacag gatctcttca ctttcgccgt agcggctttt cagcgactga atattgttgt
     6001 ccagttcaaa cagcatggcg cgctttttat catcacggat catcgactgg atccacataa
     6061 atgatgccac tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg ctggagggat
     6121 acaacaccag atcgcctgcc gggagtttta cccgatgttg tccgaaggtg tcattaacga
     6181 ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca gaaaggtcag
     6241 tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc atccacatga aaaccatagg
     6301 tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc aaggccgcgg
     6361 caaagaacaa agcatgttgg ttaaccgcgt tcagcacctc attttgcagg gcggcgtata
     6421 acgtgctgcg ggtgtcgacc tgttgattgt tcttaacttg cgcaccttgt gcgccagtgg
     6481 tgacgcgtcc atccacccat tcggcttgtt ccagttgttc gcgaaaacgg gcgacgtcct
     6541 gtggcgataa cacgccggga atgtggtaca tcatacaagt tctccaaaaa gtggggcctg
     6601 cgccccacat ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc ttggctcgcc
     6661 cgggtgataa cggtagccgc tcttattgat tgaggcgacg taatcggtat caaacaggtt
     6721 gtagacgttt agctggaagt cgagattgcg attaacccga taccccagtt tggcatcggc
     6781 gacccagtaa ccttcggtaa acgctggcgt tcccaccgcg ccgtctgaac ctttatgcat
     6841 actgccgata tagcgtgcgc ccgcacccac agaaatatca tcggttgcct gatattggct
     6901 ccataaggtg aaggcgtgct ccggggtata cggcagcgat gaggaaccat cctgggcaac
     6961 atctttgccg tttttgatgg ttgctttttg ctgggtatag ccgccaatca cctgccacgc
     7021 gggagtgata ttccctgcca cggatatctc atagccttcg acgcgtttct taccgtattg
     7081 cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg atatcagtac ggaacagcgc
     7141 ggcggtgagc aacaggcgtt tatccagaac ctgccatttg gtgccaatct cgctggtgtt
     7201 ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta ccactgccag actgcgcaag
     7261 ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca tagttaatat agacattgcc
     7321 gttttccgtc aggtgataca gcgccccggc tttccagttc accagattgc ccgacttggc
     7381 ggtgtcgacg gtggtgactg gagaaccttt tgccacacca gctgggcagg tgatggcacc
     7441 gcgtccgctg ccgccgcagg cggtggcact gtcatattca gtatgataat tatccagacg
     7501 gatcccgccg ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg cgtaaattgc
     7561 gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc gtcagtccgc cgggatgaat
     7621 gctgctgtca ggatgataaa tatttaccgc cggtaacgtc accggattaa cgccgtagtt
     7681 agtctgcgtt tcacgggtaa attccacgcc ggtactgaca tcatgaccga tagaaccggt
     7741 atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt ttattactca catctttggt
     7801 attcgccgtg cgtgaccacg tccagctatt cacatcgctg gtgggctggg taatattcga
     7861 cgccccaccc ataatcgccg tcatcaggta atcctgcttc acgcgcgacc aacgggtagt
     7921 gttgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtag cggtgtcggt
     7981 ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta tgcgtatcaa cttttccgga
     8041 atgattcagg gccgccgttc ccgcagatgg ggcagaatag cccggcaaac cgatggttgg
     8101 aatgccgccg tctggcgtgt tgtgctgggt gacatgcaga taattaagat acaaacgatt
     8161 cgctgtacca aggccaaaag cgacagaagg ggcgacgccg taacgctcat ttttgacttt
     8221 gtcgcgtccg gcatcgtgcg ttttttcgcc catcacattc aggcgcgccg cggtggtatc
     8281 accaatgacc tgattgacgt ccagcgtgcc gcggcggaac caggcgctac caatactggc
     8341 ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg atcatattga tcgagcctgt
     8401 cggtgcgctg cgcccgtagt cggtgccgga cggcccttta atcacttcga cctgctcggt
     8461 attgaaggtg tcgcgcgaga cgctgccgat atcgcgaatg ccatcaatat aaatactgtt
     8521 agaggtatca gcaccgcgca tataaatggc gtcgccagtg gtggagttac cgttctcacc
     8581 cgcaaaaaac gcacccacgc cggggacgtt tttgagtgcg tcggtaaggt tggttgcgcc
     8641 ctgatcttta atcacttgtt cagaaatcac cgtcatcgtg cgggtagtat ccgctaccgg
     8701 acgcgagaat ttcggatctg cagattgttg tggcgcataa agcgaaggcg ttgatgcttc
     8761 gacaaccagc gtgtcatccg cgttagtttg cccttcggcg gcgagtgcct gagccacagg
     8821 cgtgatgcca atacaaagac cggcaaagaa cgtgagcgaa tgaaattgtc tggcagggaa
     8881 attgcgattg ttttccattt tgcaggtgac tttttcttat atgttgaaat tttcactgcc
     8941 acgagaaagc ctctctgcgg atgcgatgcc actgttaatc tcgtgatagt gttcgtgcaa
     9001 gtgataataa ttttgataag cattatcact tggcgcgatt ttctatcacc cattgcgcga
     9061 aaaagaaata catttattta cattgcgttc atatttgatg ttttttgatt acaaaacagg
     9121 cgaaaactgc atccagaggc gaatgcagtt acgttggtgg ggttatttgt agatggttgc
     9181 agtaccgaac atctggtcat ttccgcctgc ggaattaatg acataacctt tcgcgccttg
     9241 ctcgcgcgct ttttctgcca gtttatcctc cagatcgctt aggtttgatg cgccagttgc
     9301 cgaaacggta ccggcgggcc gtagctgacc gctgtccagg ttactcatcg gttgcgcggc
     9361 ataagccgtg agagagattc cgctcaggac ggtggcaatc agtagtgtga ggcacttttt
     9421 cataatgaca tcctcataaa tacaacgggt agtgcctgat aagtgtaggt cgcctgggcg
     9481 atgaaaatga cgcaaaaaat tgacagagat acgcttattt tttgaacgat atatttttac
     9541 aaaataagac aaatcaatga catgcatgat gctgattttt atgatgaatt gccataaacc
     9601 atagccatgt taaggttttt agaaatgata aacgcagcca gatatttacc ctgtcgcgtt
     9661 atgcgggtat cttacgccgc tgcttaaagg agaatgctat gtccgcccag aaaccggggt
     9721 tgcatccgcg caaccgtcat cacagccgct acgatctcgc cacgctttgt caggtcaatc
     9781 ctgaactcag gcaattcctc acgcttacac ccgcagggga gcaaagcgta gactttgcca
     9841 atccgctggc ggtgaaggcg ctcaataagg cgttgctggc ccatttttac gccgtagcga
     9901 actgggatat ccccgacggt tttctctgcc cgccagtacc gggccgggcg gattatattc
     9961 atcaccttgc cgatttactg gcagaagcaa gcggaacaat tccagccaat gccagcattc
    10021 tggatatcgg cgttggtgcg aactgtattt atccgctgat tggcgtacat gaatatggct
    10081 ggcgttttac cggtagtgaa accagcagcc aggcgttaag cagcgcgcag gcgatcatca
    10141 gtgctaatcc ggggcttaat cgcgccattc gtctgcgtcg gcaaaaagag agtggggcga
    10201 tttttaacgg catcatccat aaaaacgagc aatacgacgc gaccttgtgt aacccgccat
    10261 tccacgattc tgccgctgcg gcgcgggcag gtagtgagcg taaacgccgt aacctggggc
    10321 tgaacaaaga cgatgcactg aactttggcg gccagcaaca ggagttgtgg tgtgaaggcg
    10381 gtgaagtcac ctttatcaaa aagatgattg aagagagcaa aggcttcgcg aagcaggtga
    10441 tgtggtttac ttcactggta tctcgtggtg aaaacttacc gccgttgtat cgtgctctga
    10501 cggacgtggg cgcggtgaag gtggttaaaa aagagatggc ccaggggcaa aagcagagtc
    10561 gctttattgc ctggaccttt atgaacgacg agcagcgccg ccgttttgtc aatcgccagc
    10621 gttaaagcgt tggctccccc ggcggtaacg gttcagccgg ggtcgcgccc ggagcaggca
    10681 gcacctgata agtctgcact ggcgcgcgaa cgcccgccag gtcgaaatgt tttttcacct
    10741 ggctgtcgag ggcaaagcgt accgtccact gtttgagcgg cagcgtagtg aacgaaacac
    10801 gcagtgtaaa cgcggtattg cttaagccga caatcccggc aaaattcggt tcaccaataa
    10861 tcagcccgcg aatttcttcg ttttccatta attccgctac cgcatctttc agtgcctgat
    10921 tggctttatc agcatcttca tggcgatcaa catcgtagtt tgccactacc gaaccaatgc
    10981 cgcggacaaa gttggcaaag gtggtaatcg aagaccacgg aatgatgtgg tacgccccgg
    11041 tatcctggcg tacgcccacg gagcgaatcg acatccgttc cacagtgccg gtcaacggcc
    11101 caatagtcac caaatctcca gtgttcatgc cgttttcaaa ctgaataaat accccggtga
    11161 taatatcttt caccagcgtt tgcgaaccaa acgagatagc cagacctaat gccccggcac
    11221 ctgccagcaa tggcgcgata ttgacgccga tttccgacaa cacaatcatg atggtgatgg
    11281 tactgataat caccgccagc gcgttacgaa acagcgtaag cagggtacgc gtgcgggcgc
    11341 tgggtagcgg gcggccatga atatccgaag ccagccggtt ttcgatcaaa ctggcgagca
    11401 ccgtccagcc aaccgccgag aagaaaagaa tgagtgcgat acggatcagg atatctacgg
    11461 ttttctgccc cgcgccgttt tgcagccagt tccagaaatc gaacaatccc catgcgctca
    11521 acagcagcat taccgccacg cagacggtca gaatacgcgc cgttttcagc gccgccgaaa
    11581 gccagccatt cagccgtttt tgcaactccg gatagttacg ctgagtatgt ggcgagagag
    11641 tgatggtttt tgccagccag cgggaaaaca taccggaaac aaacgctgcg ataccaataa
    11701 tcgccaggct gcgcaccgtt gcacccatca taaatttcag gctattgccc ggatcgaaca
    11761 acgaaaagaa aaacagcacg ataaaatagg cgcttgccag ccagtgccac accagcgcaa
    11821 aggcgcggat aaacaggctg aaaaaggcca gcgaatgctc cgcaaagttg agcaaatgct
    11881 gggtaatctc ttttttatta cgaaagatca ggtacaacgc ccagacggtc atgcacagca
    11941 taatgatgac gttcgccagc gcgccaattt gtacattcac ctgattagag ataatcggca
    12001 cggccacaat caggccataa cctatcaaac tgcttaacca gctcaggcga cgactccagt
    12061 aacgggcgct ctcgtcctga atcgtgaacg ggcgcagttc cgccacgttt gggcaaaaaa
    12121 tcaggcgtag tacggctttg aaaaattcaa tcagggcaaa ggcgttgaga aacaaacttt
    12181 gttggaaagc gatggtgcga ctgcctgcat tcaggttgtc gcttaatacc tggccgacaa
    12241 ataatgtcag ggccagtaac agcaggtcga taataaacgc cccgataatc atcgccggaa
    12301 gctgcaacca gttactgcgc tcacgatttt tttgccgcgc ccactgaccc attttgcgat
    12361 acagcggcag tgcgcacagg cgaatcagcc agtaaaaacc aaacactaaa accgctaaca
    12421 ttgaaaaatg ggtcagcgca ttgctgaagg tttgcggatt aaacggctta tgcggggagc
    12481 cggtgatatt gcgataaagt tgcccgaagc gggcggaaag ggcttcacca taatggcggc
    12541 tgacttcggt gaccttttgc agcacggttt gctcttcaac cagcgtcggc ggcacgatct
    12601 tcgggaccgg ttcagcaggt ggcgtcgcgg cgacggtgcg caactggtcg atcaactctt
    12661 tacgcgaggt gtcattatcc agcacatccg ccagtgcgcc ataggccgct tttttttgtt
    12721 cgatatccgg ttccggggcc ggttcagtcg ttgagtccgt tgtcgttgtg gttgtaacgc
    12781 cgggtatgga taccgcgtgg gcaggtgcgc ccagcaggca gaagaggatg aacaggatcc
    12841 accgcatgac tcctccagtg agaaaacaga gcaaaaagat aagtatagat gctggggaag
    12901 gggcgatttt aaatgagagg aatctggtgt gcctcccttt cgggtgaaag ggaggaaggg
    12961 atattaagca ggttgctgac aacgtgcggg cttttttgcc ggatgcggcg tgaacgcctt
    13021 atccgtccta caagttcatg caattttaat aagttgccag atatcctgta ggcctgataa
    13081 gcgtagcgca tcaggcaatg tgtcttatta agagacgtgc tgcaaaaatt cctgcaagcg
    13141 ctggctcggc gggttcttga tcaacacctg cggatcgcca tcttccgcaa tccggccttt
    13201 atcaatgaag atcaggcgcg aagctacttt ctcggcaaaa ccgatttcgt gggtcacgat
    13261 caccatcgtc atcccttctt cagccagatc ctgcataacc ttcagcactt catggcgcag
    13321 ttccgggtca agagcggaag tcggttcatc aaacagcatc attttcggct tcaccgccag
    13381 cgcgcgggca atcgccacac gctgctgttg accaccagaa agttcggaag ggtagtgatg
    13441 tgcacgttct gccagaccga ctttcgccag cagctcacgt gccagttttt ccgcctcttc
    13501 tttgttcgcg ccacgcacgc gtagcgggcc aaacataacg ttttccagcg ccgtcagatg
    13561 cgggaaaagg taaaactgct ggaacaccat acctgcttcc tggcgaatca ggcgctcgtc
    13621 aactttcgga tcgttaacct tcaggccatc gacaatcaga tcgccggagg tgatttcttc
    13681 cagtttgttg atgcagcgca gcagggtcga tttaccggaa ccggacggcc cgataatcac
    13741 cacgacttcg ccctgggcaa tgttcaaatc gatattgtgc agcacctggg ttgggccaaa
    13801 gtgcttggag acgtttttaa attcaatcac aggattttca tccttctttc cagacgacgc
    13861 agaataaagc tcagcaccag cgtaataatc agatagaaca ccgccacggc gctccagatc
    13921 tcaagggcgc ggaagttacc ggcaataatt tcttgcccct gacgggtcag ttccgccacg
    13981 ccgataacaa taaacagcga ggtgtcttta atgctgatga tccactggtt acccagcggc
    14041 ggcagcatac gacgcagtgc cagcggtaaa atgacgtagc gaatggtttc ccaacgtgaa
    14101 agaccgagcg ccagtcctgc ttcacgaaaa cctttgtgga tagacagcac cgcaccacgc
    14161 gtgatttccg caatgtacgc gccggagttg atcatgatgg tgaccaccgc cgcagtaaat
    14221 gggtcgatgc gtaagtcgtt aaacgccatc ggcagggcga aataaataaa catcacctgg
    14281 acgacgatag gtgtgccgcg gatcacttca ataaagacca gcgcgacgtg gttggctatc
    14341 caaccaccga aggtgcgtgc aaaacctgcc agcaatccga ttaccagacc gccagccaga
    14401 ccgaggaccg aaatccacag ggtcattttg gcaccttcaa tcagaagcgg aatggcaggc
    14461 cagatggcac tccagtcaaa ctgcatatgt tgttcctgtt accgtggtgt aaataatcaa
    14521 attcagggta attaatggcc ccggagcggg tttgcgcccc tcaccctaat cctctcccca
    14581 gaggggagag ggaactgcca gtgcgcttta caggtgtagc gttattattt cggttcagta
    14641 ccgaaccatt ttttgtagat ttcgttgtaa gttccgttct cgcgcagggt tttcaacgcg
    14701 ccgttgactt tgtcacgcag ctcgtcgcta cctttcggga acgctatacc gtattgctgc
    14761 gcttccagag agtcacctac cgctttgaac tgaccgttac cggcggtttt gatgaagtac
    14821 agaatgtttg gcgtatcgtg cagaacggcg tctgcgcggt tggtgcccag ttccatatag
    14881 gcgttatcga tgttcgggaa ctgacgcaga tctttagttt tgatgtttgc tttcgcgtaa
    14941 tcaacggagc cagtaccgct cttcacagca accactttcc cgtcgagatc tttcacgctt
    15001 ttcacatcgt tattgttagc tttcaccatc actaacaggc cgcttttgta gtagccgtca
    15061 gagaaatcga tcgctttttt acgctcgtcg gtgatggtaa tgcccgccag cgccagatcg
    15121 acgtttttgg tttgcagtgc cggaatgatc ccactgaaat ccatcggctt cagttcgtaa
    15181 tccagcttca gctctttagc gatggcagcc cacagatcaa cgtcaaagcc cacatattta
    15241 tcgccctgtt taaattcaaa cggaacgaag gcggtatccg tcgcgacaac taatttttta
    15301 tccgcggcat gagaagaaac cgcaaaagcc agggtcagtg cagccagtga aacttttaat
    15361 acagacttca tagcatttcc ttttttctat ccacgggacg atcccctgcg tgacattcac
    15421 atatatgaaa aaatcgtgcc agttttgcaa ctcattgatt tttcgagatg cggcaaaaac
    15481 gtgatgcacg atttataggg caataccctg aagatgcacc attctggggc accaaactgg
    15541 tgcgctaaaa ttgtgcactc aacacagtga ttatttagcg taaaacctga tgaaaaaaca
    15601 atctttatgt aacgattgtg tgatgatgtg gatacaaaaa atttacttaa tcagctgggg
    15661 atagcagatg gatgcactaa ataagtgcgt tgaggcgggc tgcaaaacaa aacggcctcc
    15721 tgtcaggaag ccgcttttat cgggtactaa agttctgcac catcagcgat ggatttattc
    15781 gatgttagac tcgataaacc acaggaattt atccaggtcg cgagacgcgg cggtcaggat
    15841 atctgcggtg tcgtcatctt tcgcttcgcc aatcgctttg cgtacgtcat tagcgacgat
    15901 tgcgtaacgg tcagccagtt ctttcaggtg atcctgaacg ttgtggatgt ccagcgggta
    15961 acttttcagc ggggttttgc tgttgataac ttgagtggtc cccagagcta caccgcccag
    16021 ctgcactgca cgttctgcca tggtatccag atgatcgatc agtgcagtgc ggaagccatc
    16081 cagcatttca tgtacggcaa tgaagttagc gccgcgcatg ttccagtgcg cttgtttggt
    16141 aatcaaagaa agatcaataa actggataac ctgacgattc agcaactcta ctgttgcttt
    16201 tttctcgctg tcagagacat cgttgcgggt ataaagcaga ttggtcgctt ttgatttaac
    16261 taatttagcg gtactcataa tttcatatcc tcttgatgtt atgtcccagt aattaacgag
    16321 actaagtata gcaccggcta tgtgttccgc tattctgg
//
LOCUS       DAZGBC010000111        15943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGBC010000111 DAZGBC010000000
VERSION     DAZGBC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.112"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(122..1171)
                     /gene="yahK"
                     /locus_tag="ACHK1J_003426"
     CDS             complement(122..1171)
                     /gene="yahK"
                     /locus_tag="ACHK1J_003426"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO7912657.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1258..1335)
                     /locus_tag="ACHK1J_003427"
                     /pseudo
     CDS             complement(1258..1335)
                     /locus_tag="ACHK1J_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1548..2930)
                     /gene="yahJ"
                     /locus_tag="ACHK1J_003428"
     CDS             complement(1548..2930)
                     /gene="yahJ"
                     /locus_tag="ACHK1J_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO7912658.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2940..3890)
                     /gene="yahI"
                     /locus_tag="ACHK1J_003429"
     CDS             complement(2940..3890)
                     /gene="yahI"
                     /locus_tag="ACHK1J_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO7912659.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTEVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STVLLAREIHADILVITTGVEKVCIHFGKPEQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPESLPAALRGETGTHIIKT"
     gene            complement(3940..4194)
                     /locus_tag="ACHK1J_003430"
                     /pseudo
     CDS             complement(3940..4194)
                     /locus_tag="ACHK1J_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4220..5638)
                     /gene="yahG"
                     /locus_tag="ACHK1J_003431"
     CDS             complement(4220..5638)
                     /gene="yahG"
                     /locus_tag="ACHK1J_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7912660.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFEQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(5638..7185)
                     /gene="fdrA"
                     /locus_tag="ACHK1J_003432"
     CDS             complement(5638..7185)
                     /gene="fdrA"
                     /locus_tag="ACHK1J_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7912661.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETSVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIDAI
                     KEAKAIAAAGGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(7175..8038)
                     /gene="yahE"
                     /locus_tag="ACHK1J_003433"
     CDS             complement(7175..8038)
                     /gene="yahE"
                     /locus_tag="ACHK1J_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7912662.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     HDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRFRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8078..8683)
                     /gene="yahD"
                     /locus_tag="ACHK1J_003434"
     CDS             complement(8078..8683)
                     /gene="yahD"
                     /locus_tag="ACHK1J_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO7912663.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQVLIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8941..9438
                     /gene="yahC"
                     /locus_tag="ACHK1J_003435"
     CDS             8941..9438
                     /gene="yahC"
                     /locus_tag="ACHK1J_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7912664.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLCQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            9530..10462
                     /gene="yahB"
                     /locus_tag="ACHK1J_003436"
     CDS             9530..10462
                     /gene="yahB"
                     /locus_tag="ACHK1J_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7912665.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(10504..11592)
                     /gene="pdeL"
                     /locus_tag="ACHK1J_003437"
     CDS             complement(10504..11592)
                     /gene="pdeL"
                     /locus_tag="ACHK1J_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7912666.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
     gene            complement(11734..15705)
                     /gene="ehaA"
                     /locus_tag="ACHK1J_003438"
     CDS             complement(11734..15705)
                     /gene="ehaA"
                     /locus_tag="ACHK1J_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO7912667.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LALSKDVAVSEDYTAVFGKDNQTIDYRISVTDNANLVIDATDSSRPRLTLASGGGLDI
                     TGGKVHINGSLNFLLKGTGFLNVANAGSELYADDLYESYSNITHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     VPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDIAQWSHNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3649 a         4362 c         3789 g         4143 t
ORIGIN      
        1 acaatttgaa tttacgttat aggttttact gctactttga tattatttcg ctctccttaa
       61 ctaaaaagtt aagtggagag cgaagttaat atgttaaacc acagggtatt tattaatttt
      121 ttcagtctgt tagtgtgcga ttatcgataa caaaacgata tttcacatca ccacgcagca
      181 ttcgctcata ggcttcatta atttgatcgg cccgaatcat ctctatatca gccacgatgc
      241 catgttcggc gcaaaaatcg agcatctcct gagtttctgg aatgccgcca atcatagaac
      301 cggctatcgc acggcgtttc atgatcaggt tgaaaacttc cggcgattta tgcggtgtcg
      361 caggcgcacc aaccagcgtc atggtaccat cacgcttcag caaggtggta aaatcgtcga
      421 gattatgtgg cgcagctact gtattcaaaa tgaaatcgaa actcttcaga tgagccgcca
      481 tctcatcggc attgcgtgag ttaacaactt catcggcccc cagggctttt gccgcttcgc
      541 gttttgcctc agaagtggta aatgccacca catgtgcccc catcgcgtgg gccagcttga
      601 tccccatatg tcccagaccg ccgatgccga ccacgcccac ttttttaccc ggtccggcct
      661 gccagtggcg caacggggag taagtagtga tccccgcgca cagtaatggg gctaccgctg
      721 ccagttgctc ttgcgggtga cgaatacgca gaacatatcg ctcatgaacg acgatctgtt
      781 gtgagtagcc gcccagcgta tggcccggtt cgtccggcgt cggcgagtta taggtgccgg
      841 tcatgtgatc acagtagttt tccagcccgt cttcacactc ttcgcaatgt ttacaactgt
      901 cgacaatgca gccgacaccg accagatcgc ccggcgcatg tttttctacc tgatcaccaa
      961 cggctaccac acgccccaca atttcatgac ccggcacgca gggataaacc gtccccgccc
     1021 actcggaacg gacctggtgg agatcggaat ggcagacgcc acagtaagcg atttcgattt
     1081 tgacatcatt cggtcccggt tcacgccggg tgatatccat cggttcaagt ggttgtttag
     1141 cggaatatgc accaacagct ttgatcttca ttgtgtttac tcctgattag ctatgtgtat
     1201 ttggcgacgc ctgatatgtc atcgcggcaa aacgcgtcca ttgaatatag ccaatattta
     1261 aaccacccga tccagcccca acatgccgcg agcctgaatg aaacagagca gcaacgttcc
     1321 ccataaggcg agcgtctata agtgtgatat ttgctatccg taaaagacta aaatcggcaa
     1381 aactgatgat cttttcacgt agacaaatag ttatctacac aacgtcaata agggcatatt
     1441 cctcaaagat aatgtccatt tctgccgaca cactcatatt cagattgccc cactccctgg
     1501 ggcttaaatc tcttactatt caccgttgca ggcgtttcaa atgcgcatca acctgccaca
     1561 ctcccccaca ccagttgccc tttatggaac gttgcggttc tcggcgagat acgcgccacc
     1621 gcctcggcgg aacaggaggc gtccaccagc acaaagctgg cgtcatcctg cgcttttggc
     1681 catacacgct cgcctttttc gttgagcggc aatacatcac cagtggctaa aaacagcgag
     1741 cgggagaggt tctgttcgtt aggacgaata tagagctgcg cgtagagatt ggctttttcc
     1801 agcatgtcgc ccagaccata aggcgaccag tggtcgataa cgctgtcagt gccagtcatc
     1861 acttttacgc ctttgtcgtg caactgtttg agcggcatat gcagcgtgcc aatcggcacc
     1921 gtcgaggcga tagaaatttg ctgcgccgcc atccgattcg ccagttcatc gacctgttgt
     1981 tcgttgagcg tagccagcgc aaaggcgtgg ctgatggtca gcttgccctt cagttgcggc
     2041 gttttctcta ccgtttcaac catataattg atggctgcca cgcccgacgg agtggtttcg
     2101 tgcaggtgaa tatcgacgcc tttgtcgtag tccagcgcaa tctggaacat ggtatcgagg
     2161 gatttttcca tcgcgccatc gacactggtc gggtccagcc cgccgacgta atgcgcaccc
     2221 gcctgcattg cttcacgcat taagggttcc gatttcgaca gcagcaaacc gtgctgcggg
     2281 aaggcgacga tttcacactc aaagcccgcc tgacgtcgcg ccagcaccgc ctgcaaattt
     2341 tgcagatttt tcaggccgga aaccggttcg atattgcagt ggctgcgggc aatggtggtg
     2401 cctttcgact gtaataaatc gatcagtttt tcagcccgtt cctgcgtgta cggttgcagc
     2461 tccggcagca ttttctgctc gagtttgatc atgtcctgga tggtggtgcc tgccgggcga
     2521 ttgagcgagc gccacggccc gccgtaaaag gttttgtcga gatgaatatg catgtcgcgg
     2581 gtggtgggca gcatcagctt atcgccagcg tcatagtgcg gcagcgtggc atccgggtgc
     2641 tgcttattct cgcgcagcgc gacaattttg ccgttctgaa tctccacggt ctggcgcgcc
     2701 gtgcgggtct gcaccgccac gccattttcg tagtcaaatc cagtttccag cagcacgttg
     2761 tcgagatagt aatgcgggtc agtgattttc atggtgttat tcgcgtcgca gtttagggtg
     2821 ccagctaccg ccgcatgggc gagcggcaca gaggtaccaa acagcgcggc ggcggtgacc
     2881 atcttaccgc tctggctcag aaactcacgg cggctattgc tttctttcat cttacgtcct
     2941 tacgttttaa taatatgagt gcccgtctca ccacgcagcg ccgcgggcag gctttccggc
     3001 gtggtgataa tcacttcttt gccgccttgt tccaggaacg tcaggctggc gatgattttt
     3061 ggcaacatgc tgccgggcgg gaaatgtcct tcctgcatat agcgagtcat ggtggcaata
     3121 tccacccgat cgagcgcctg ctgctcgggt ttgccaaaat gaatacacac tttttccacg
     3181 ccggtggtga tcaccagaat gtcggcgtga atttcacggg ccagcagcac ggtagagaga
     3241 tctttgtcga taaccgcgtc cacgctttgg taatctcccg cgtcagtacg caccacagga
     3301 attccgccgc cgcccgcgcc aatcaccaca aagccttgtt ggatcagcgc cttaatggcg
     3361 ggtgcttcga caatacgttt cggttccggc gaggcgacca cgcggcgata gccccgtccg
     3421 gcatcttcaa caaaacacca gtcagggttt gccttttgta attcgtcacg ctggctttca
     3481 ctgaagaatg cgccgatggg cttggtggga tgagcaaatc ccggatcgtt tttatccact
     3541 tccacctcag tcaccacggt gacggctttt ttctcgccgt gacgcgccag ccggttattc
     3601 agcgcctgtt ggatcagata gccgatgccg ccctgcgtat ctgccacaca gttcgccagc
     3661 ggcgttaatg gcagcccttc gcgctcgtgg gcaatctccg cacggcgtaa atccagcccg
     3721 acctgcggcc cgttaccgtg ggtcagcaca atgtcgtaat cggaagccag catttccagc
     3781 accgtatcgg cgacggcttt caccgcctcc gcctgatgct caatcgactg gctggcgtta
     3841 tctttgataa tgctgttgcc accaatggca acgaccacaa gctctttcat gttgttatgt
     3901 ccgctacaga gggtgatggg gttatgacca acgccagact gcctgatgcg ctacgcttat
     3961 caggcctaca aattttcgtg ccattcgtag gccggataag gcgttcacgc cgcatccggc
     4021 aatggtgaca aatgcctgat gcgctacgct tatcaggcct acaaattctc gcgccactcg
     4081 taggccggat aaggcgttca cgccgcatcc ggcaatggtg acaaatgcct gatgcgctac
     4141 gcttatcagg cctacagatt ctcgcgccat tcgtaggccg gataaggcgt tcacgccgca
     4201 tccggcatga acatgtaggt cagacgccgt aacgttcgca ccagccaaga atggctttct
     4261 cgaagcaggc aaaaggtgga tgcacaatgc ccgcaccaat catgccgacg cccgcatctt
     4321 tatgggcgat ggcggtgttg atcactggca gaatgccgct gctacccacg cgggtgatgt
     4381 cgattgccga cggcacgccc ataaaaccga gcagcggaat ggtgacgttg gggttttcgc
     4441 caagggtgat ttcgcgcatc tgacgggaga aatcgatagc ctcttccacc gtgccaccca
     4501 ccagcgcgac gattgcaggc gcggtcgcca tggcaaatcc gccaataccg taggtttccg
     4561 tgatggcgct gtcgccaata tccagccctg aatcttccgg cttataaccg gcaaacatcg
     4621 ggccgatgac ctgctgcgcc gggccggtaa accactgccc cggcaggccg ctgacccgca
     4681 ggccgaactc gacgccatta cgcgccatgg tggtgaccac ggtgctgtat tcaatgccat
     4741 gcgccgcatc catcgccgct ttacacatcg ccatccacgt cgggccggag aagtaatcgc
     4801 tactggcaac aaactcaaac acttcgcgct gttgcgcgac ggaatagccc gcctggataa
     4861 tccccggcgt cagcgcctga atcagcagcg tcgtcccggc gttattgcgg ttatggcact
     4921 catcgcccat atgcagcgcc tgcgccagca ttaagcgcag atcgatttcg ccgataattt
     4981 tcatcgcgtc gcgcagtatt ggtccctgca catcacgcat ccagttcagg cggtcaatca
     5041 cgctctggtc gttagcgccc atacgcaaaa tcttcgccat ctgctcgctc atgttggtat
     5101 aagcgatgtt gccgtaggtt ttgtttttta cgatgtgcat aaacatcgag gccgaggtaa
     5161 cacccgccat cgatcccacg cagtcgtgct cgtgacacgg cgagaaagtg atctccccgg
     5221 aagccgccag ttcagccgcc tcgtcgagat ctttcgccag tccttcgaac accagcgctc
     5281 cggtgaccgc gcctttcatc gcgccgcaca ttttttccca ggtgatcggc ggcccggcgt
     5341 gaagaatggt tttcggcgtc atgcccggca ccacgttaat cgcctgctca aaaccaatca
     5401 gcaccggatg cgactggata atgcgctcca gcgcctgctg gttagcggcg gcgattttgt
     5461 ctgccagcgg cgaatcggca atgttgtcca gcgcctgcac cacctgcata ttgccctgcc
     5521 ccggcggcgt ccagtcgagt tgagtcactt ctacatgctg ctttttcagg tcatcgctaa
     5581 acatggcgat gccgacgtta ataacgttca atggttggct aaacagtgac tggctcatca
     5641 ggcttcctcc cctttgcaga taaattcacg cgccagcaat ccggtattgg tgctgctgct
     5701 cgccagaatt accccggcat caagcagcat ctggctttgt tgttctaacg atggcgtatc
     5761 gagatcggtg cccagcacat aggcgagaat gatcaactcg cgccctccgg ctgcggcgat
     5821 cgcttttgct tctttgatcg catcgatggt ggaaccaacc ggatcttcgt gcgatccaaa
     5881 tccgagcaca aaatccatca cgatcaccgc cacttctgga tcgcgcgctt cttcgatcaa
     5941 gcgactgatg cggttggtgg ggtcaatcat tggatgcggc ttgccgttgg tgaagtcgtc
     6001 atcgccaaag tcgaggaagg tgtgtttgat gctgcggttg atatctttca ggcggaattc
     6061 cggatcgggc tgaatgttgc tgtagacatc gccatgtttt tccatcaccg cgaacatggt
     6121 ttcgtcgcac aacgtgccgc cgcagaacag gccacgaatg tatttctgct gcggttgcag
     6181 acgcgcacgc acatccgcaa tcaacggctg gttaagcgta tgtaggtcga gattttcctg
     6241 tttcacgccg gagagcatca ccgcttttag cgccgcttct ttgctgccgc gagcaaactg
     6301 tagcccctgc tcatccactg acgtttcgcc acgaccgagg aagcagacca ccaccggctt
     6361 gcggcaggcg cgcgcacgtt ccagcacttt gcgggccacc gcaggcgcag gcggtttgga
     6421 gatcagcgca atgatttcag tttgcggatc gttttccagc atcccgatgg cgtcgagcat
     6481 catcaggccg ccgattttct cgctcaggtc gcgcccgccg gtgccaatca gttgtgaaac
     6541 gccgccacca aattcatgaa tgcggacgct cagctcctgg ctgccggtgc cggatgcgcc
     6601 aacaataccg atgttgccgc gacgcacggc gttaccaaaa cagagcgccg cgccgttgat
     6661 aatcgccgtg ccacagtctg gccccatcat cagcagccct ttttcgtggg ccagttgctt
     6721 gagcgccagt tcatcttcaa ctgagacgtt atcggaaaac agcatcacgt tgaggtcatt
     6781 ttgcagcgcc tgacgcgctt cgcgagcggc aaacagaccg ttgaccgaaa tcaccgccag
     6841 gttactttcc gggatatgct ttttggcgct ggcaatagtg gcgtaacgcg cctcgtgcga
     6901 gccgctttgc gctttgctgt tgaacagttc ttcaatctcc accagcaact gctcgttgtc
     6961 cgcccccgat ttaccattga tgacaatcat caggtcgccg tttttcgcct gttccagctc
     7021 cggcgtcagc agtcctaaat ttttcagcac acctttgttc atttcggtcg ccatcgccac
     7081 aaatgcctgc tcgacgccgt cgagtttatt tgcacgcgtg gagatagaca tcagcgagac
     7141 agaatcaaaa taggtattcg gtttaatgac tattttaact gacataattc cctccgtagg
     7201 tttggctcag cgcacaacca tcggccatgc catacagcat gtcgcagccg gaactggagc
     7261 caatatgttt aattttttct attgcctgag ttgcgttccc agggatgtca taaataatgt
     7321 tgtgaataaa acgatgaata ttttcccgga agcgttgttg taatgcggct tccagcgtta
     7381 tggcacttaa taacgtggta ttatttctgc cgcgttgcaa gccgagataa aattcctctt
     7441 tgtattttcc cgccggatgc ccggaaataa ataaaataag tgccagacct gttaaataat
     7501 catcggctga tggcgtaagg ccaatcccta agcccatcat actggcgacc gctgaggcga
     7561 tattttgttt atcgattact gcctgtataa ctgcccgtcg tttaatatgt aattctttat
     7621 ttaacgcctg ataaaacgga ttatcgccgc gataattaaa cagggtttca ttttccccca
     7681 ggtgctgatg aatgatgtcg tgccactgtt gccaggcgat aaggcgaaaa ttcacagcgg
     7741 tcatttgcaa ggttggggac agccaacgcc gacaacgact catctctata tgaagatgtt
     7801 gaccaaccgt aataccttga tcgtcaaact gaaccttatc accatgccgg aacagatcat
     7861 caaagtgagt gagtgccaac cgacaactgt ttggcgcatt gtcgtgctct tcacaaagca
     7921 gcgtcagcaa ctgctggcga gccgggagtg cgatatttac cgctctggca aagacctgtt
     7981 caacctgtcc ttgcccccgc tgcgccagaa aatccgcatc cgcagttagc gcccacacac
     8041 accgtttttc tggtgtgttg atagcaagcc tcccggttta tgcacctgcg gcaatcagta
     8101 actgcgcaat ctcttcaaag ccccgttccc gcgccagttc cagcggcgtt ttgccatatt
     8161 tatccgtcat gtgcgggctg gccccgtgtt ccagcaataa ctggacaatc gcctgctgtt
     8221 taataccacc atcattaagc acaatcgctt ccagtagcgg cgtccagcct acatggttgg
     8281 tctggttaac gttaatctcc gtatgtgcca aaagttcttt tacaatactt aaatggcctt
     8341 tttcacaggc aggcgtcagg ccgacaccgc caaaacgggt tacgcaatta agatcgggtt
     8401 tagccggtaa aataattcgt agtagcgtta aatcatcgtt cagacagcta attaaaaaag
     8461 gatttaaaca ggtatgatct tgtttattaa tatccgctcc ggcgtcaatt aatacctgaa
     8521 cgcaagcata ttgctgatat aaacttgcca gcgtaattgc cgttttcccc tgacgatcgc
     8581 aggtattaat atcgacaccc agcgcaaggc aggtttttac tttatcgata tcaccctgtt
     8641 gtgcagccaa taaatagtcg gcaggtagat tttttatact cattcacatt cccctggttt
     8701 ttgtggctgt tttcgatgaa ttgagaatag caacgataat ctgataaaag aacccgctta
     8761 aatttaattc gccgtatttc gcctttttgt tgaatgatgt tcaacaatct tttcgcagtg
     8821 tgcgatagtg caatatttat ttctttgacc cgataccctt tcactcaatc aggttttatt
     8881 tctctatagt ctgcacatat aagcgcagct ttcgtccatg tctaaccaga ggggaacaag
     8941 atgaacggac tcacggcaac gggtgtcacc gtcggtattt gtgcagggct ttggcagtta
     9001 gtgtcttcac atgtcgggct ctgtcaggga tgggaattat taggaaccat tggcttcgtc
     9061 gccttttgca gtttttatgc cgcaggcggc ggtaaatcgg ggtttatcag aagcctggcg
     9121 gttaattatt ccgggatggt gtgggcgttt ttcgccgcgc tggctgccgg ttggctggcc
     9181 tctgtaagtg gactgtccgc gttctgggcg agcgtaatta ctaccgtacc cttctcggcg
     9241 gtggtggtct ggcaagggcg tttctggtta ctgtcattta ttcccggtgg ctttctcggc
     9301 atgacgctgt tttttgccag cgggatgaac tggacggtga cgttactggg ttttctggcg
     9361 ggtaactgcg tggggataat ttcagaatat ggtggacaaa aattaagcga agcgacgact
     9421 aaacgcgacg ggtattaaaa gttattcaaa aatgattatc tatctttaac cgatacctct
     9481 acagtcatag ctggagaaat tcatagtaga taatggcggg aatggtttaa tgaactcaat
     9541 ttttaccgaa gagaatctgt tggcatttac taccgctgcg cgttttggca gtttcagtaa
     9601 agccgcagaa gagttgggtt taaccacttc cgccattagc tacaccatta agcgtatgga
     9661 gacggggctg gatgtggtgc tgttcactcg cagtacccgc agcattgagt taacggagtc
     9721 cgggcgttat tttttccgca aagctaccga cctgctgaat gattttcacg ccatcaaacg
     9781 cagtatcgat actatttcgc agggcataga agcgcgtgtg cgcatttgta ttaatcagct
     9841 gctttatacg cccaaacaca ccgcgcgctt attacaggtg ctgaaaaagc agtttcctac
     9901 ctgccagatt accgtgacga ccgaagtgta taacggcgtc tgggatgcga ttattaataa
     9961 tcaggccaat atcgccatcg gcgcacccga tacactactg gacggcggcg gtattgatta
    10021 cactgaaatt ggcgcgattc gctgggcttt tgctatcgcc ccggatcacc cgttagcctt
    10081 tgtgccggaa cccatcgccg aaagccaact gcgtctctac cctaatatta tggtggagga
    10141 caccgcgcat acgattaata aaaaagtggg ctggttgctg cacgggcagg agtcaattct
    10201 ggtgccagat ttcaacacca aatgtcagtg ccagatttta ggtgaaggga ttggtttttt
    10261 gccggattac atggtccgtg aggcgatgac gcaatccctg ctggttaccc gacaaatcca
    10321 taatccgcgc caggattcgc gcatgttact ggcgactcag cattctgcta cgggtcaggt
    10381 gacgcagtgg attaaaaagc aatttgcgcc aaatggcata ttaacgggga tctatcagga
    10441 tttactgcat cgggagaatt aatcgctgcc aacctcccga tgcgctgtga atagcggttt
    10501 acatcaacca ccagctttca ttacccattc agaaacaaat ttattacccg gtactggcgg
    10561 agagtacaaa tagccctgca aaaagtgaac gcctttaccg atcattaaat ccgcctgctc
    10621 ctgggtttct accccttccg ccacgatact cagaccaggc ttacgcgcca gttcgacaat
    10681 attgtccaca atatgaccgg atatttcgtc gacactcgcc atttgcacaa atgacttatc
    10741 gatcttaata aaatcgaccg gaaacgcctg cagataacga taggtcgcat atcccgtacc
    10801 aaagtcatcc agcgcaaagg taatgttatg ctgatgaagg ctgtcaaata tcgctctggc
    10861 ttctggcgtt accggaatag ggttacgttc cgttagctcg agaaccagtt tgattttatc
    10921 gtttcctaat ttcttgacca ggttcaggca ttctttttca aatcccgcgg ccaaaaaaca
    10981 acccgccgag acgttgatac caatatggaa attatccggc agcaaatgtt ttaccggcat
    11041 cagaatatcc gcagtctgtt tcatcagttg gcgggtcatt atgacaataa gaccggatga
    11101 ctccgccaga ggaataaact gatccggcgg gatgattccc gtttgtggat gttcccagcg
    11161 gacaagcacc tcacagcccg tcagtacccc agtctgcgcg cagaaaaccg gttggatcca
    11221 cggtttgaat tcgtggtttt ccagcgccag actgatggct tcaggcgtga cgatatggtg
    11281 acaacggtta tcagggatat aaaaattatt tttatttccc gtgccactga tgacttgcgg
    11341 atgatattga aaaaagatat cccgccagaa ggtaatatca ctctggatgc ctagtttttc
    11401 atacagctgc ttcttttgat gtgaaattgt ctttgcactg cgatttctgt actgtgagat
    11461 ttctgtaacc gacattccct ccattagcag cttgagtaaa agccgttctt tttcggacac
    11521 gataccaggc aacgtcaaat tcaccgttgc cccgaactct tccaaaaaaa cactcaaatc
    11581 atgcgaagcc attgaacaca ccttgattaa atcttgtgcg ccagctaaat ctgcgagtct
    11641 tcactctgac ggataaagct ggctgcaaga aaatgacaca gagagccgaa ttatctcccg
    11701 ttcggctctc tctcctttta ctcattattc agatcagaat tgccacttaa tgccaaccat
    11761 tgcagaggtg tcattatatc ccctatcggc aacctgaacg cccacattcc cccagacatt
    11821 caggttggca tttagctgtc cttccacccc ggtttttatc tcagcaatat ttcgggctcc
    11881 atcctgagtg acggacacgc catccatact ggtgctgaaa tccttactgt tatgcagcca
    11941 gttcacttct acaaacggct ggaactcgcg ggatttaccg tcatccattt tatggtggct
    12001 cttcagccag gtttttacgc caagtcgggt ttgaacattg ccatcaccgt tgctatgaac
    12061 gagggttccg ttgctttcgc ggtgcttatc ggctttgact cccatccagg taacctgtgc
    12121 ttgcggctga acataccatt cattacgcgt tccctggctg ccattaaatt cagctaattt
    12181 gtgtttgtat ccagcttcca gtgaagcggt aaatcctttt gatttatagg attcactttg
    12241 taagtcatcc cctttcactg tgttatcaaa ccagctgtac tgcgcccaac tgtcgagata
    12301 cgcgccatta cgcgactcgt catcggcata ccaggtggca tagaggcctg tgctatatcc
    12361 gttcacactc gcttttgcac gataaccggt tcgcgaggaa atggttttgc tgtcgctgtt
    12421 gccatatccc gccatgaccc caacatgcca gctgtcgctg ccgttatggc tccactgcgc
    12481 gatatcgcct cccagttgca gaacatagcg attgctttgg gttttcagct ggccgctgcc
    12541 atcacgccat ttattatgac caccttcatg gcgcatccac atagtggttt gtttctgctc
    12601 acccgtcacc atgtcggtat agtacgtatt acccagacgc tcatgcagac gcgtggtgaa
    12661 catggtattc gctgctgcaa ggttcgcaat gtagctaccc gcctccggtc gcaggtcatt
    12721 atccacattc agatccgggc ccggcgtagg tgtcgggtta gggttcggct ctggattcgg
    12781 attcggcacc gggtctggct ccggctgcag ttcaggagaa tcactgccgc tggtcagata
    12841 ccagttacca ctatttgccc cttgtccacg cgcgagagtg tagtcatacg cccccgcaac
    12901 gatacgacca gcctgaacaa attcgccctc agacttaccg tctacatgga taagttcgat
    12961 accgttaagg gttttcgcac ctgtaccgcc agcgttattc accgtaacgg cagtcgtgcc
    13021 gctagtatcg ccctcgacaa ccaatttatc ggttactgag tcatcgccat tcagtaccgt
    13081 attcattact agttgaccgt tattaccgtg gtagttcccc ttcaccacta acacattgcc
    13141 agcttgctgt cctgacattc cggtcatcag cgtaccgttg ttggtcagat tgccgcccag
    13201 ggtgtaattc acagacgcag ccgctctttt ttcagcgcta ttattcaacg ccagggtacc
    13261 gttgttctca acgtttgcgc tacccaatcc ctgcgcatga cttaccagga cattagccga
    13321 attagcaatg ttcacattcg ccgtcaggtt gctgttatcg cccgcgatgt ccagcgtgcc
    13381 gccctgaata ttcagctctc cgctccccgt taacgaaccg gttgaagtgc ccccgttggt
    13441 aaccgtcaga ctgccgccat ttaagctcag cagtgaatcg gcgctgccat tgagcgtgcc
    13501 cacggtctgg ctgtgaccat tcatatccag ttcagtctct gccgccagac gcagttcatg
    13561 ggtattaccc aacacgttat cgttattcaa caaaagcgtc ccactgcgca gatcggtaac
    13621 accggtatag tcgttgtctt tgttagaaag cgatacggtc tgacccttct ggctgctgaa
    13681 tgccaggtca ccactgccgg taatctttgc gctgagatcg gcggcattct cgcttttacc
    13741 gttagagctc agcaccagag catcgctgtc ggtagcgtgt aaatcaagct gggtcaggcc
    13801 gtaaccgata tacagaccgt cattgtttac cccactcgtc agacgatagt cgtaagtgcc
    13861 gacggccgct ttttgcccat tctgctgaat atcacgttgg gcgctgctgg agaggatttg
    13921 cccattttta tcctgcaatt gcagattgcc cgcatcgccc agaacttccg caccttgcgc
    13981 atcaaccaac ttaatggtgc tgttaccatc atcgacttca gtgagcgata acgcagagtc
    14041 aatatcacga gatactgagc ttacaacgtc actgtcagaa acctgaatca caccctcgcc
    14101 gcgcaaatcc agcgttttac taacgttgac cgtcccttct gtcatctgtg cacctgctgt
    14161 cagggcacca aaatcaacgg ttccgccgga gaatcccaaa gtgctaatgg agtgagcgcc
    14221 cttacccact gttaccaggc ttccctgacc cgcagtgatc ccgcctttct gcagaaactc
    14281 agcattcttt tccagattaa atgtcgtgtt ctggaacgcc agtgaaccct ggaaccagtc
    14341 tgtataagca aactcatcga aggagaattc gtgattcccc atgtttattt caacaatacc
    14401 tctaccttca agatcgttat caaataccca atctttattg gcattgattt gcagtttgcc
    14461 atcaacatcc aggtccgcac ggccgatgtt cttgtcttca gaaataaccg cgacagcacc
    14521 agaagcgaca tcaatatatc cgctgaatgc atagttattg ccggtcagtg ccactgtccc
    14581 actgtcgata cttattgtac cattgccgga aatcttattg gcaaaggtca tgctatcgtc
    14641 agccttcagg tgtaatttcc cgcggttgtt gatttctgct gtacctatat tttgggtgat
    14701 ccctaaagcg ccattttgct caattttgac tttaccgcta aaggcggagt tatccccggt
    14761 taagtaagtc gtaccattga gcgcattgat atagcccagg ccctcgctgt cactgacaat
    14821 atccgcggaa atagtcgcgt ttttatccgt gtggtttaag gtgattttct tctcggatgt
    14881 ctttgcccac acaaactcaa ttttttcggc atcaataatc cctgcagcct tcgctgcgct
    14941 tccttcctgt gcacctaacg ctaactcaga gttggtgctt aaactaatag tcggcgcaga
    15001 gattttacca ccttccgaaa cggcaagtgt tgtatcggag ttttgatcat aataacccag
    15061 gctaattttc tcactggcat taacttcacc gccgttatta actgacaggt actgattacc
    15121 accgtaactg ccgtaaacgc ccataaagaa ggttttagaa tttacctggc tgccttcacc
    15181 gctgacattt ccctgcaaat aggtcagacg gctggtgccc ttaacatgga ttttgccgcc
    15241 gttggagaca ttaaaatagc ctctatcgtg ggtaatatta gagtaagact catataaatc
    15301 atcagcatat aactcgctgc cagcattggc gacgttcaga aaccccgtcc cttttaacaa
    15361 aaagttaagc gagccattga tatgcacctt ccctccagtg atatccagcc caccaccaga
    15421 cgcgagagtc agacgtggac gagaagaatc tgtcgcatcg attaccagat tggcattgtc
    15481 ggtcactgaa atacggtaat caatagtctg gttatcttta ccgaatacag ctgtgtaatc
    15541 ttcacttaca gcgacatctt tagacagtgc cagagatgaa tataaactta tcgcgctaat
    15601 taaaacagcc cgggactttt ttccttttac tctgctcccc agctcagagc agacgctcca
    15661 acagttacgg gacctgttcc actttagccg atatatttta ttcatcacaa tctccagcaa
    15721 tacatccaaa taaaaccaag attgcataaa cagcaacgca ttaaatgcca ttccggttac
    15781 tgttcatcgc atataaaaat tcaaaaattt tgcatagcat tggcaaccac atatctgagt
    15841 atgtagttca gagggtaata aaatagcaag ttactactga aaacttattt taagttaaca
    15901 ctcttaactt tatcattaca ttcccccccc agtctctaat acc
//
LOCUS       DAZGBC010000112        15542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGBC010000112 DAZGBC010000000
VERSION     DAZGBC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.113"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..311)
                     /gene="yehE"
                     /locus_tag="ACHK1J_003439"
     CDS             complement(30..311)
                     /gene="yehE"
                     /locus_tag="ACHK1J_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO7912668.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(573..1682)
                     /gene="apbC"
                     /locus_tag="ACHK1J_003440"
     CDS             complement(573..1682)
                     /gene="apbC"
                     /locus_tag="ACHK1J_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO7912669.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1814..3847
                     /gene="metG"
                     /locus_tag="ACHK1J_003441"
     CDS             1814..3847
                     /gene="metG"
                     /locus_tag="ACHK1J_003441"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO7912670.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3988..4182
                     /locus_tag="ACHK1J_003442"
                     /pseudo
     CDS             3988..4182
                     /locus_tag="ACHK1J_003442"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            6715..7791
                     /locus_tag="ACHK1J_003443"
                     /pseudo
     CDS             6715..7791
                     /locus_tag="ACHK1J_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7804..10836
                     /locus_tag="ACHK1J_003444"
     CDS             7804..10836
                     /locus_tag="ACHK1J_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO7912671.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHITWGKVGSNGQSQVKSFAD
                     ATAAEKAELKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNNETSSELLDEIVS
                     TFGIEYATETVIFWWQIDFDFDYKDELLTFFINYADSSKDSYRRNNDKFSLRLRKHLS
                     LAEEEVWQNCIAKLLVALPDISLFRQPLIAILMPEIPEVAHEIVHRLHQVADVPQLEM
                     LKLVATDPFTLEILENYQYIDVFNTYGASWSATVLREQGIAALARLAPYAEEDNCGDV
                     LKCINHPLAITALIEAADTNKRCYERLIKSAENFPSATIAALAEALVKKDDKRKHTLL
                     VSLLNANPLLVEKLVIGLSSQAANLLQQCQQLLNSRNEDFANSEILPMVLVSPPWETQ
                     NKNNDNSPVGKYPAKIPPLPLFYQPPLWTRPKIKNSSKVLPDSALQHLGEMLRFPQEE
                     ALYPGLLQVKDACTADSLAEFAWDLFTAWLTAGAPSKESWAFTALGVLGNDDTARKLT
                     PLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIA
                     ESRELTVAELEDRLAPDLGLDDNGSLLLDFGSRQFTVSFDETLKPFVRDVSGSRLKDL
                     PKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAMCLRRRWSPENFQLFLVE
                     HPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEGDISIGIPHVLE
                     ISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAGRKCPSGRVMGL
                     ANKGWIKGTPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWE
                     GKAESYGWGSNSTQEAQLSVLDAITASELINDIEALFE"
     gene            10846..14478
                     /gene="yehI"
                     /locus_tag="ACHK1J_003445"
     CDS             10846..14478
                     /gene="yehI"
                     /locus_tag="ACHK1J_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO7912672.1"
                     /translation="MDKELPWLADNAQVELKYKKGKTPLSHRNWPGEPVPVITESLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEKSCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQKNRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNNPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAVQRDLARQWILHWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEHLLKQSPIEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRHFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHLLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            14539..14856
                     /gene="yehK"
                     /locus_tag="ACHK1J_003446"
     CDS             14539..14856
                     /gene="yehK"
                     /locus_tag="ACHK1J_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO7912673.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         4182 a         3866 c         3739 g         3755 t
ORIGIN      
        1 ctaaatgcaa ttaaaatcag gttgccaaat taatcgtagc gatttaacac gattcgacgt
       61 ttgctatcac tacctgcaac aaccacttct gtcgttacac ctttgacggg cgaagaaacc
      121 tgatgacggg tatcgacatt atacgttaac tgaccacaat gctgttgagg ttgactatta
      181 accatagcca tactgcatgt tgggggactg atcctgccat taatcgttaa tgtggcagtc
      241 tctgaagaga acgcgggaga agccaatcca taggcaagaa aaataattcc tgacagccaa
      301 tacttattca ttgaacgtta tccctgtagt aaaggttatg cctggataga atgagtgcat
      361 aacaaactat agctgtacat ccactacaca gcacgaagga tgataatgaa gcattgcctg
      421 tacgatcaat cgactttgta gagtttcgga cgaaggtccg cagaatattc gcagtattaa
      481 ataagtgttc attaatgcgg caaaaacaaa tacaccgcat caatataaca tctctgcggt
      541 gtatgaagaa taaccagaat ggttaatagg cgttagaccg cgcggaagga aatctcgcct
      601 ggaatgactt caccctgcca gtagagctga gctgcaacgc ggtcagccag ctggcggtag
      661 atagcggtaa attcgctctc cggacggctg ataaccgttg gcgtgccttt atcgagatcc
      721 tcacgtaagg agatgtgtaa cggcatctgc cccagcagct gtgtatggta tttttcagcc
      781 agtttctctg caccaccggt accgaaaatc ggttcatggt gaccacagtt actgcaaata
      841 tgcacactca tgttctcgac aataccgagc accggaacct cgactttctc gaacatcaca
      901 atgccttttt tcgcatcgat caacgcgatg tcctgcgggg tggtgaccac gactgcacca
      961 gttactggaa tgttttgcgc cagcgtcaat tgaatgtcac cggtgcctgg cggcatatca
     1021 agcaccagat agtcgagatc cggccacaag gtttcctgca acatctgcat taacgccttg
     1081 ctcgccatcg gtccacgcca caccatcgca ttgtcgtcag tgaccagata accaattgaa
     1141 ttggttgcca ggccatgaga cataatcggt gccatatggg taccgtccgg cgaagttgga
     1201 cgctgatttt ctgcgcccag catggtggga attgatgggc cgtagatatc ggcgtccaga
     1261 atgccgactt tcgccccttc cgccgccagt gccagcgcca ggtttaccgc cgtggaggat
     1321 ttacccacgc cgcctttacc ggaactgacc gcaatgatgt tcttcacgcc attaataccc
     1381 ggctgattct tcacgcgttt taaggtggcg atattgtgcg acagtttcca gtcgatagct
     1441 ttcgccccgg tgatacgcag cagttcggcg ctacattgct cttttaactc ttcaaacgcg
     1501 ctatgccaga cgaatggcat aaccagttcc acatgcaacg tgtcgtccat ccaggcgaca
     1561 tggtgcaacg ctttcagcgt ggtgaggtta tgcttcaggg ttgggtgctg aaaattggcc
     1621 agtgtcccgg cgaccattgc gcgcaaggct tctggtgatt tggcctggga ttgttcgttc
     1681 atcccgactc cttttttgta tagataaacc atcagctgat agtttacctg aagaatatag
     1741 agaagtactt acttaacatt ttcccatttg gtactatcta accccttttc actattaaga
     1801 agtaatgcct actatgactc aagtcgcgaa gaaaattctg gtgacgtgcg cactgccgta
     1861 cgctaacggc tcaatccacc tcggccatat gctggagcac atccaggctg atgtctgggt
     1921 ccgttaccag cgaatgcgcg gccacgaggt caacttcatc tgcgccgacg atgcccacgg
     1981 tacaccgatc atgctgaaag ctcagcagct tggtatcacc ccggagcaga tgattggcga
     2041 aatgagtcag gagcatcaga ctgatttcgc aggctttaac atcagctatg acaactatca
     2101 ctcgacgcac agcgaagaga accgccagtt gtctgagctt atctatactc gcttgaaaga
     2161 aaacggtttt attaaaaacc gcaccatctc tcagctgtac gatccggaaa aaggcatgtt
     2221 cctgccggac cgttttgtga aaggcacctg cccgaaatgt aaatccccgg atcaatacgg
     2281 cgataactgc gaagtctgcg gcgcgaccta cagcccgact gaactgatcg agccgaaatc
     2341 ggtggtttct ggcgctaccc cggtaatgcg tgattctgaa cacttcttct ttgatctgcc
     2401 ctctttcagc gaaatgttgc aggcatggac ccgcagcggt gcgttgcagg agcaggtggc
     2461 aaacaaaatg caggagtggt ttgaatctgg cctgcaacag tgggatatct cccgcgacgc
     2521 accgtacttc ggttttgaaa ttccgaacgc gccgggcaaa tatttctacg tctggctgga
     2581 cgcaccgatt ggctacatgg gttctttcaa gaatctgtgc gataagcgcg gcgacagcgt
     2641 aagcttcgat gaatactgga agaaagactc caccgccgag ctgtaccact tcatcggtaa
     2701 agatattgtt tacttccaca gcctgttctg gcctgcaatg ctggaaggca gcaacttccg
     2761 caagccgacc aacttgtttg ttcacggcta tgtgacggtg aacggcgcga agatgtccaa
     2821 gtctcgcggt acctttatta aagccagcac ctggctgaat cattttgacg ccgacagcct
     2881 gcgttactac tacactgcga aactctcttc gcgcattgat gatatcgatc tcaacctgga
     2941 agatttcgtt cagcgcgtga atgccgatat cgttaacaaa gtggttaacc tggcctctcg
     3001 taatgcgggc tttatcaaca agcgttttga cggtgtgctg gcaagcgaac tggctgaccc
     3061 gcagttgtac aaaaccttca ctgatgccgc tgaagtgatt ggtgaagcgt gggaaagccg
     3121 cgaatttggt aaagcagtgc gcgaaatcat ggcgctggct gatctggcta accgctatgt
     3181 cgatgaacag gctccgtggg tggtggcaaa acaggaaggc cgcgatgctg acctgcaggc
     3241 gatttgctca atgggcatca acctgttccg cgtgctgatg acttacctga agccggtact
     3301 gccgaaactg accgagcgcg cagaagcatt cctaaatacg gaactgacct gggatggtat
     3361 ccagcaaccg ctgctgggcc acaaagtgaa tccgttcaag gcgctgtata accgcatcga
     3421 tatgaagcag gttgaagcac tggtggaagc gtctaaagaa gaagtgaaag cagctgccgc
     3481 gccagttact ggcccactgg cagatgatcc gattcaggaa accatcacct ttgacgactt
     3541 cgccaaagtt gacctgcgcg tggcgctgat tgaaaacgca gagtttgttg aaggttctga
     3601 caaactgcta cgcctgacgc tggatctcgg cggtgaaaaa cgcaatgtct tctccggcat
     3661 tcgttctgct tacccggatc cgcaggcact gattggccgt cacaccatta tggtggctaa
     3721 cctggcacca cgtaaaatgc gcttcggtat ctctgaaggc atggtgatgg ctgccggtcc
     3781 tggcgggaaa gatattttcc tgctaagccc ggatgccggt gctaaaccgg gtcatcaggt
     3841 gaaataatcc cccttcaagg cgctgcatcg acagcgcctt ttctttataa attcctaaag
     3901 ttgttttctt gtttgttcaa cgctcttaat ttttttcatt aactgctagc atccgcttat
     3961 aactggatat aaatacaggg aacgcggatg aagagttata tctatcagga cgaaaaatca
     4021 cataaattct gggcggtgga gcagcaggga aacgagttgc atatcagctg gggaaaagtt
     4081 ggtacccaag ggcaaagcca ggtaaaaaat tttgctgatg ttgcagcagc ggaaaaagcg
     4141 aaactgaagc ttatcgcgga gaaaacgaaa aaaggctatg tggaaaatgc ttcagcaaac
     4201 gtgcatatcc cccccattac caaagccact cctgaagttg aaacttcccc tgagagtaaa
     4261 aaccaacgcc cctggctggc tgatgatgcg gtcattcggc taactgacga tattaatcga
     4321 tttgcttttc ctcatcgctc tcgccccaga gagatcaatt atctgcacaa agatggtcaa
     4381 atatggcagc tcatcgcgaa taacaccaag gcatatgatc ccgccaacaa ttaccgttct
     4441 tatccagaaa actggcagca ggcttttgct gagttacaaa tgcgagttca gggtaatcaa
     4501 cagacaggga gtgtgcaatc tgatgcagca ttgctctgga gtttctggaa ctcttactct
     4561 gcagatgaac tgatcgatga cttagtcatt cgctgtggtc tggaaagtgc cgttgaaatt
     4621 gcccttctcg ctcttcaact tagatataaa ccagtaaaag ccgacgtcac caccatcatt
     4681 ccccctgatc tcaaagcaga atcgctaccg aactggcatc aacgtctatg tcatcatctt
     4741 tcactcgctt cagaagatga gtggcaacgc tgtgtagata aggtactcgc ggctattccc
     4801 tcgctatccc cagcacggca accttttgcg gcgttactga tccctgaacg cccggatatc
     4861 gccaatgcta tggcgctaca ttatgcagat caaaacgttc cggcgatgac ctggttaagt
     4921 atgatggtaa gcgatgatgt tgccctggcc accctggaaa aatatggttt cccaccgcta
     4981 tataatgatt ttcgcaaata tctggctacg ttgctggcaa ataatggaat gcgtggtgta
     5041 agccggatac ttctcaagct acctgtcgac tatcccgtaa aatacacgga ccttttcact
     5101 cacatacacg ccaatgctga agatcttgtc aaatggctat ggaaaacgaa tcacccagat
     5161 gcgattcaaa ttctgatcct cggtgtaaat ggcaagaaaa agcacctgga atacttaagc
     5221 aaagcctgcc aaaaacatcc cgctgcggct attgcggctt atgctacttt gctggcaata
     5281 catgaagata aagagtggcg taaagcgctt gtcaaactga ttaccgccac gccagagtta
     5341 gtatgcgacg tcattccctg ggttaatgct aaagcggcag gcatcttatc tgaatgtcgt
     5401 ccgcagccag tagctgaaga atgtgaatat gccactgtgg atatgttgcc ggagctgctt
     5461 gtttcgccgc cctggatgac caaagagaaa aagaaaaata cgcctgtgtt tgatcttcca
     5521 gtgcttccag ttccttctgt ttctgatgtc actccagaga ttaccaaaaa actgacccgt
     5581 acttaccttg tcacccactt ccagcaaata gcccaacagc aggcgacaaa acaaacattg
     5641 tttaccgacc tgccgccgat caaaaaagca agctgggaga aacacttaat accgcttaca
     5701 ccggagcaac aaatactgtg gcatcttggt tttgaaaaat ggcgggaaag cggagaaaaa
     5761 atatacgaaa aaattccggc gccacaaagc gccgttgatg ccctgctacg cttcgatttt
     5821 ccagcactca atgctgaatt tgtgcaatat cataacaatg catacaaaag ctggaatctc
     5881 attgccctct gttacctgcc tggtcaacag gccatttctt ttcttaacca gatagtcaaa
     5941 gaagataact actctggtga gggaaatatt ctggcaattt ttggtagcgc cgccatacct
     6001 gcatttatgg cctgcctgca aggtgatcca cgacgtctgt gcttcttccc ctttttcctc
     6061 ggtgtcagtg aactggcctt accgatggcg caacaattac agaaaaaaat gtcttatgaa
     6121 gatgcgcgaa actggctaac tgattatcca cgccatgccg ccgccgggct acttcctgtg
     6181 gccctgggca aaaaaggaaa agatcgtgat tgtgcccgcc aggcgcttcg tctgctggtg
     6241 aatttaaacc agcgagaaac gattgaggaa atcgcgcaag gatataatca acctgatgtt
     6301 ttagctgcgc tggcaacatt gttcgatagc gacccactgg aagaatatcc cgctaaaatc
     6361 gccccactgt ccggctttta tcagttcacc ttgtggcgca gaccacggct taaaagtaac
     6421 aacctgcctc tgtcagatga cgctatgcgc cacctcggca ctatgctgag ctttcctcgc
     6481 gacattaccg cctacgctgg gctggatatc attagagaaa tcttcacccg cgaatcactg
     6541 gctgaatttg gctgggatct gtataccgcc tggacagaag ctggcgcacc cgcaaaagaa
     6601 aactgggcat ttacttcgct tgggatttta ggcaatgacg acaccgcgcg taaattaacc
     6661 ccgcttatcc gcgcctggcc tggggaatcc cagcataaac gggcggtgtc cgggctggat
     6721 gtattagctg atattggcag cgatgttgcg ctaatgctgc ttaatggcat cgcgcgaaaa
     6781 attaaattca aagcattaca ggaacatgcc cgcgagaaaa tcaacatagt tgctgaaaac
     6841 cgtgggctga ctatggctga actggaagac cgcctggctc cagatttagg gcttgatagc
     6901 agtggctcgc tgatactgga tttcggcccc cgcaaattca ccgttggttt tgacgaaacc
     6961 ttaaaacctg tagtgtgcga tgcaaacggc aaagtcctga aagatttacc taagccaaac
     7021 cagagcgatg ataaaacact ggcaactgac gcagttaatc tcttcaaaca gttgaaaaaa
     7081 gatgttcgtg ctatcgccag ccagcaaatt acccgtctgg aacaggctat gtgccagcgc
     7141 agacgctgga cggcagagca gttccgcctg tttctggtgg agcatccgct ggtacgtcac
     7201 ttaacccgac ggctgttatg gggggtttat aacgatgaaa acgctcttat cacctgtttt
     7261 cgcgtggcag aagacagcac ttacagcgat gcgcaggatg agttattcac gctgccagca
     7321 ggaaacatcg gtattccgca tgtgctggaa atatcccctg aatccgccgc tgcattcagg
     7381 caaatttacg ctgattacga actgcttccc cctttccaac agctcgagcg aggtagctat
     7441 caccttgctg ataatgaacg taatactcac gaactgacac gctggcaagg acggctttgc
     7501 caggccggac gcattgtcgg gctggaacgc agaggctggc aacgtctgga agaaagcggc
     7561 agcgtttatg cgatgcgcaa aacaacccct catggcgatc tcgaacttga aacggaacct
     7621 ttttcattaa tttatggtga aacggggtat ggcgaccagc taccggttga aagcgtaaaa
     7681 atcacctcgc cagatgatcg ctacggtaaa caatcctcac tcactttctc catgctcgac
     7741 gacatcaccg ccagcgagct gattaacgac atcgaatcac tgtttgatta aaaggattta
     7801 acaatgaaaa cctatattta tcaggacgaa aaatcacata aattctgggc ggtggagcag
     7861 caggaaaacg agctgcatat tacctggggt aaagtcggta gtaacgggca aagtcaggta
     7921 aaaagctttg ctgatgctac ggcggcggaa aaagcggaac tgaagctgat tgcggagaag
     7981 gtgaagaagg ggtatgtgga gcaagtagac gctaacgctc actcacatga tgcctcagca
     8041 aagaaagtcc tggtcacaga ggaaaaagcc tcaataaatc gccaggcagc aactaatggt
     8101 ttaccctggc tggcagacaa cgatcccatt attcttcccc cgaatattgc ccgccatgcc
     8161 ctcagccacc gcctttggcc gggagagcca gtaaaaaaac caaccaaacc cgagaaacaa
     8221 aatttaattt cccgattggc aaatcgtact tatcattatt ataaaaatct gactttcgat
     8281 tacagctcat gctctgatga atgggctgat gcgattagcc aggcaatcag cttaattaag
     8341 gcccaaaaga ctgagatacc cgcgcgtact atggcggtac ttacttttct ggaaatatgt
     8401 ttttgtcagg gctggaataa tgaaacctca tccgagttgc tggatgaaat tgtaagcacc
     8461 ttcggtatcg aatacgcaac tgaaactgtc atcttctggt ggcaaattga tttcgatttt
     8521 gactataaag atgaactttt gactttcttt attaattatg cagatagcag caaggacagt
     8581 tatagaagga ataacgacaa atttagttta cgtctgcgta aacatttatc actagcagaa
     8641 gaagaagtat ggcaaaattg cattgcaaaa ttactcgttg cgttaccaga tatatcactc
     8701 tttcgccaac cgttaattgc cattttaatg ccagaaatac ccgaagtggc ccatgagatc
     8761 gtacatcgtt tacaccaggt cgcagatgtt ccacaacttg aaatgttgaa actggtagcg
     8821 acagatccat tcacgctcga aatactggag aactatcaat acattgatgt atttaacacc
     8881 tatggtgcca gttggtcagc tacggtatta cgggagcaag gtatagcggc actggctcgc
     8941 ctggctccct atgctgaaga agataattgc ggtgacgtac tgaaatgtat taatcatccg
     9001 ttggccatta cggcactcat cgaagccgcc gatactaata aacgctgtta tgaacgacta
     9061 ataaaaagtg ccgaaaattt tccttctgcc acgatagcgg cattagccga agcattggta
     9121 aaaaaagatg ataaacgcaa acatacgttg ctggtttcat tacttaatgc aaatccttta
     9181 ttggttgaaa aattagtcat cggattatcg tcgcaagcgg caaatttatt gcaacaatgt
     9241 cagcaattgc tgaatagtcg caatgaagat tttgcaaact cagaaattct accaatggta
     9301 ctagtctcgc cgccctggga aactcaaaat aaaaataacg acaacagtcc tgtgggtaaa
     9361 tacccggcga aaatcccccc tttaccgttg ttttatcaac ctccattatg gactcgcccg
     9421 aaaatcaaaa atagcagcaa ggtactgcca gatagcgcac tccagcacct cggtgaaatg
     9481 ctccgcttcc ctcaggaaga ggccctgtat ccgggattat tgcaggtgaa agatgcctgt
     9541 accgccgact cactggctga atttgcctgg gatctgttta ccgcctggtt gactgcgggc
     9601 gcaccgtcga aagagagttg ggcgttcact gcgttaggcg ttcttggtaa cgatgacacc
     9661 gcccgcaaac tgacgccatt aatacgcgcc tggcctggtg aatcccagca taaacgcgcc
     9721 accgttgggt tggatattct cgccgctatc ggtagtgata tcgccctgat gcagcttaac
     9781 ggtattgccc agaaactgaa attcaaagca ttacaggagc gggcaaaaga aaaaattgcc
     9841 gacattgccg aaagccgcga actcacggtg gcagagcttg aagatcggtt agcaccggat
     9901 ctcggtctgg atgataacgg ttcgttgctg ctggatttcg gctcacgcca gttcaccgtc
     9961 agctttgatg aaaccttaaa accgtttgtg cgtgatgttt ctggcagccg tctgaaagac
    10021 ctgcccaaac caaacaaaag cgatgatgaa tcacaggcga acgatgctgt taaccgctac
    10081 aaattgctga aaaaagatgc gcgtaccgtc gccgcccagc aggtagcaag gctggaatcc
    10141 gccatgtgcc tgcgccgccg ctggtcgccg gaaaacttcc agctcttcct ggttgagcat
    10201 ccgttggttc gccacttaac ccgccgtctg atttggggtg tttatagcgc cgaaaaccag
    10261 ctactggctt gctttcgcgt agcggaagat aacagctaca gtaccgctga cgatgatctt
    10321 ttcaccctgc cggaaggcga tatctctatc ggcattcctc acgttctgga aatatcacca
    10381 acggatgctg ccgcctttgg tcagcttttt gccgactacg aactgctacc accgttccgt
    10441 cagcttgacc gtaacagtta cgccctgaca gaagccgagc gcaacgccag cgaactgacc
    10501 cgctgggcag gcagaaaatg cccgagcggt cgggtcatgg ggctggcgaa taaaggctgg
    10561 ataaagggca ccccgcagga tggaggctgg atcggctgga tgatcaaacc tctgggccgc
    10621 tggtcgttaa tcatggaaat cgatgaaggt tttgcggtag gcatgtcgcc agccgaactg
    10681 agtgccgagc agctcttaag caagctgtgg ctatgggaag gcaaagcaga aagctatggc
    10741 tgggggagta attcaacaca ggaagcgcag ctctccgtac tcgatgccat caccgccagc
    10801 gagctgatta acgatattga agctctgttt gaataaggaa agtgcatgga caaggaatta
    10861 ccgtggctgg cggataacgc ccaagtggaa ctgaaatata aaaaaggcaa aacaccactc
    10921 agccatcgca attggccggg cgaaccggtg cctgttatca ctgaaagcct catccagaca
    10981 ctgggtgatg aattgctaca aaaagcagag aagaaaaaaa acattgtctg gcgttatgaa
    11041 aatttttcac tggagtggca gtccgccatt acgcaggcca tcaacttgat cggcgaacac
    11101 aaaccctcag tcccggcccg cacaatggcg gcgctagcct gtatcgcgca aaatgacagc
    11161 caacagttgc tcgacgaaat cgtccaacaa gaggggctgg aatatgcgac tgaggtggtg
    11221 attgcacgcc agtttattgc acggtgttat gagagtgatc ctctggtagt cacattgcag
    11281 tatcagaacg aggactatgg ctatggttat cgctcagaaa cctataacga attcgatctc
    11341 cgactgcgta aacacctctc tctggcagag aaaagctgct ggcagcgttg cgccgacaaa
    11401 ctcattgccg cactgccagg aataaccaaa gttcgccgtc cttttattgc gctgatcctc
    11461 ccggaaaagc ctgaaatagc caatgaactc gccagtcttg aaagctcccg gtcaagtctc
    11521 cattcaaagg agtggttaaa ggtcgttgct actgataata cggcggtaaa aaaactcgaa
    11581 cgatactggg gcctggacgt atttagcgat cgcgaagcca gctacatgtc acaaaaaaac
    11641 cgcttcggct acgcggcctg cgcctccctt ttgcgcgaac aaggactggc agccgttccg
    11701 cgcctggcaa tgtatgctca taaagaagat tgcggcagtc tgctggtaca aattaaccat
    11761 ccgcaagtca tccgcacctt gctgttagtt gctgataaaa acaaccccag cctgcaacgt
    11821 gtggctaaat acagtaaaaa cttcccccat gcgacgctcg ccgcactggc agaactgctg
    11881 gcgttaaaag aaccaccagc ccgccctggt tatccaatca tcgaagacaa aaagctgcct
    11941 gcacagcaaa aagctcgaga tgaatactgg cgtacgctgt tacaaacgct gatggcatcg
    12001 cagccacaac tggcagcaga agtgatgccg tggttaagta ctcaagccag ggcagtgctg
    12061 aagagttatt tatcggcact gccaaaaccg gttattgatg gcaccgataa cagcaatctg
    12121 cctgaaatcc tcgtttcacc accgtggcgt agtaagaaaa aaatgacagc tccacgtctt
    12181 gatttggcac cgctcgaatt aaccccgcaa gtttactggc aaccaggcga acaagagagg
    12241 cttgcctcca ctgagtctgc ccgttatttc agcacggaat ctcttgcgga acgcatggaa
    12301 caaaaaagtg ggcgagttgt attacaggaa ctgggttttg gggatgatgt atggctgttt
    12361 ctgaattata tactccccgg aaaactggat gctgcacgca attcactcat tgttcagtgg
    12421 cattactacc aggggcgggt tgaagagatc ctgaatggct ggaactcccc ggaagcacaa
    12481 ttagcagaac aggcgctccg cagcggtcac atagaagcgt taattaacat atgggaaaat
    12541 gacaactact cacgttatcg tccggaaaag agtgtctgga acctgtattt attggcacag
    12601 ttgccgcgtg agatggcttt gaccttctgg ctgcgtatca atgagaaaaa acatctgttc
    12661 gcgggtgagg actattttct cagtatcctc gggttggatg cgctaccagg tctgatgttg
    12721 gctttttcac atcgtccaaa agaaacattt ccgttaattt taaattttgg cgcaacagaa
    12781 ctggccctgc ccgttgcccg cgtctggcga cgtttcgcgg tgcagcgtga tctggctcgc
    12841 cagtggattt tacattggcc ggaacatacg gctactgcac ttatccctct tgtcttcacc
    12901 aaatccagcg ataatagcga agctgcatta cttgccctgc gtttacttta cgaacaggga
    12961 catggtgaat tgctgcaaac cgtggcaaac cgctggcagc gtacagatgt atggcctgcc
    13021 ctggagcatt tacttaaaca gagtccaata gagatttacc cggcacgcat tccaaaagcc
    13081 cctgatttct ggcatccgca aatgtggtcc aggccgcgcc ttatcactaa taatcaaact
    13141 gttaccgatg acgctctgga aattatcggc gaaatgctgc gctttaccca ggggggacgt
    13201 ttttatagcg ggctggaaca actgaaaacg ttctgccagc cacaaacgct ggcagctttt
    13261 gcctgggatc tcttcactgc gtggcaacaa gctggtgccc ccgcaaaaga caactgggca
    13321 tttctggcgt taagtctctt tggtgacgaa agcacggcac gggatctgac gacacagatc
    13381 ctcgcctggc cacaagaagg caaatctgcc cgtgctgtca gcggcctgaa catccttacc
    13441 ctgatgaata atgatatggc gctgatacag ctgcatcata tatcgcaacg ggctaaatcc
    13501 cgccccttac gtgataacgc ggcggaattt cttcaggtgg tcgcagaaaa tcgcgggcta
    13561 agccaggaag agctagcgga cagattagtc ccaaccctgg gccttgatga tccgcaggcg
    13621 ttgagttttg attttggtcc ccggcatttt accgttcgct tcgatgaaaa ccttaacccg
    13681 gttatctttg atcagcaaaa cgttcgccag aaaagcgttc cccggttgcg cgccgatgac
    13741 gatcaactga aagcgcccga ggcactggcc cgactaaaag ggctaaaaaa agatgccact
    13801 caggtgagca aaaacctgct cccgcgtctt gaaacggccc tacgtaccac tcgacgctgg
    13861 tcgctggcag attttcattc tctgtttgtt aatcatccct ttacccgtct ggttacccag
    13921 cgattaatat ggggggttta tccggcaaat gaaccgcgtt gtttactcaa cgcctttcgt
    13981 gtggccgcag agggggagtt ctgcaatgcg caagatgagc caattgacct gcctgcggac
    14041 gctctgattg gcattgccca cctgttagaa atgacagtag aaatgcgcag tgaatttgca
    14101 cagctttttg ccgattacga aattatgccg ccttttcgcc agttgtcgcg gcgcacggtg
    14161 ctgctcacac ctgacgagtc aaccagtaac agcctgactc gctgggaagg taaatccgct
    14221 accgttgggc aacttatggg aatgcgatac aaaggctggg agtcaggcta tgaggacgca
    14281 tttgtctatg acctgggtga gtaccggctg gtccttaagt tttcacccgg ttttaaccac
    14341 tacaatgttg atagcaaagc gctaatgagc ttccgttctc ttcgagtgta ccgtgacaat
    14401 aaatccgtca cttttgccga acttgatgtg tttgatttga gtgaggcgtt aagcgcacct
    14461 gacgtcattt tccattaaaa cacagcgggc agtgatgcaa ctgcccgtta tcaacacgac
    14521 ttaccagcgg ggaaagcgat gatcgtgcaa aaagagctag ttgctattta cgattatgag
    14581 gtccctgtac ctgaagatcc gttttccttc agacttgaga tccataaatg ctctgaatta
    14641 tttacaggtt ccgtctatcg actggagcga ttccggctac atccaacatt tcatcaacgt
    14701 gatcgagaag atgctgaccc gctaataaat gatgcgttga tttatataag agatgagtgt
    14761 attgatgagc ggaaattacg aggtgaatca cctgaaactg taatagcaat ttttaatcgt
    14821 gaactacaga atatattcaa ccaagaaata gaataatata ctctagataa ttaaagtcgc
    14881 agcacatgca acttgaagta tgacgagtat agctttctta aagggggaaa cggtattccc
    14941 ccttagatgc gtttcaattt gcccgttact tcaacaaatc aatcaaccgt aacagagcca
    15001 ctaataactc cagtacggca atgagcacgg ttaacttttg tgtcagcgtg tccatccgct
    15061 ctccttatta aggagcgcaa caccagcacc ttacccttac actgcctgtc gatactggtt
    15121 tacgtatgtt ggtacgaaac gttgctctcc ggatcagggc accgcctgcg gcaccacccg
    15181 tccagcagcc aggacttaaa attggtccga tccggcacaa cctcccaacg ggcaggatta
    15241 taaatctcat tcactgaatt gcaatggagt gtggatatgg ttataattta ttcgttggta
    15301 cgaaatgttg cacaatcagc aagttagcgt cactgtagta aataaaagaa cctgcctcac
    15361 cagcaggttt ttttattttc tgcgatctac tttccagata ttttttgcca atagcccacc
    15421 ataaatattc acatcccggt caaatggtgc gttgaatata aaatcgatga acttataaat
    15481 aaacatgagt tatatgatga attaataaaa acagtaatca ttttattaaa gggggaagcg
    15541 gt
//
LOCUS       DAZGBC010000113        15501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGBC010000113 DAZGBC010000000
VERSION     DAZGBC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.114"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..681
                     /gene="yceJ"
                     /locus_tag="ACHK1J_003447"
     CDS             115..681
                     /gene="yceJ"
                     /locus_tag="ACHK1J_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO7912674.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            685..1260
                     /gene="yceI"
                     /locus_tag="ACHK1J_003448"
     CDS             685..1260
                     /gene="yceI"
                     /locus_tag="ACHK1J_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO7912675.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1302..2354)
                     /gene="trhO"
                     /locus_tag="ACHK1J_003449"
     CDS             complement(1302..2354)
                     /gene="trhO"
                     /locus_tag="ACHK1J_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO7912676.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPACAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2579..3499
                     /gene="lpxL"
                     /locus_tag="ACHK1J_003450"
     CDS             2579..3499
                     /gene="lpxL"
                     /locus_tag="ACHK1J_003450"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO7912677.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3671..4897
                     /gene="mdtG"
                     /locus_tag="ACHK1J_003451"
     CDS             3671..4897
                     /gene="mdtG"
                     /locus_tag="ACHK1J_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO7912678.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4980..5354
                     /gene="msyB"
                     /locus_tag="ACHK1J_003452"
     CDS             4980..5354
                     /gene="msyB"
                     /locus_tag="ACHK1J_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7912679.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5355..5582)
                     /gene="yceK"
                     /locus_tag="ACHK1J_003453"
     CDS             complement(5355..5582)
                     /gene="yceK"
                     /locus_tag="ACHK1J_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7912680.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5755..8298)
                     /gene="mdoH"
                     /locus_tag="ACHK1J_003454"
     CDS             complement(5755..8298)
                     /gene="mdoH"
                     /locus_tag="ACHK1J_003454"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO7912681.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPLDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8291..9826)
                     /gene="mdoG"
                     /locus_tag="ACHK1J_003455"
     CDS             complement(8291..9826)
                     /gene="mdoG"
                     /locus_tag="ACHK1J_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO7912682.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPSNSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10219..11376
                     /gene="mdoC"
                     /locus_tag="ACHK1J_003456"
     CDS             10219..11376
                     /gene="mdoC"
                     /locus_tag="ACHK1J_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO7912683.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSLIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11384..12865)
                     /gene="clsC"
                     /locus_tag="ACHK1J_003457"
     CDS             complement(11384..12865)
                     /gene="clsC"
                     /locus_tag="ACHK1J_003457"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO7912684.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12807..13340)
                     /gene="ymdB"
                     /locus_tag="ACHK1J_003458"
     CDS             complement(12807..13340)
                     /gene="ymdB"
                     /locus_tag="ACHK1J_003458"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO7912685.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENSHLYERLLTQQGDE"
     gene            complement(13435..13746)
                     /gene="ymdA"
                     /locus_tag="ACHK1J_003459"
     CDS             complement(13435..13746)
                     /gene="ymdA"
                     /locus_tag="ACHK1J_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO7912686.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNSQVASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13867..14199)
                     /gene="csgC"
                     /locus_tag="ACHK1J_003460"
     CDS             complement(13867..14199)
                     /gene="csgC"
                     /locus_tag="ACHK1J_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO7912687.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14258..14713)
                     /gene="csgA"
                     /locus_tag="ACHK1J_003461"
     CDS             complement(14258..14713)
                     /gene="csgA"
                     /locus_tag="ACHK1J_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO7912688.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14754..15209)
                     /gene="csgB"
                     /locus_tag="ACHK1J_003462"
     CDS             complement(14754..15209)
                     /gene="csgB"
                     /locus_tag="ACHK1J_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO7912689.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3860 a         3965 c         3680 g         3996 t
ORIGIN      
        1 aattcaaaat tattgaaata aaaataaata tcattcaatt tgttattttt gtgttgcaat
       61 tcattctccg acacgccatt ttctttctct cgcaaattcg ttttggatgc acttatgtca
      121 ttcacaaata cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca
      181 attattgtct acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat
      241 ggctggtatc acaaagcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg
      301 ctggttattc gcgttctgtg gcgcgtcata tctccaccgc cgggtccgct gccgagttat
      361 tcgcccatga ctcgtcttgc cgcaaaagct ggacatctcg cattgtacct attgctgttc
      421 gccatcggca tcagcggcta tctgatctca actgccgatg gtaaaccgat cagcgttttt
      481 ggctggtttg acgtccccgc gacccttgcc gacgctggcg cacaggcaga ctttgccggt
      541 gccctgcatt tttggctcgc gtggagcgtc gtagtattgt ccgttatgca cggatttatg
      601 gccctgaagc atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg
      661 tcatctgact atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc
      721 ctgatgttct ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag
      781 cacgcctttg ttaacttccg catccagcat cttggctata gctggttata cggcaccttt
      841 aaagatttcg acggtacttt tacctttgat gaaaaaaatc cggctgccga taaagtgaat
      901 gtgacaatta acaccaccag cgtcgatact aaccacgccg aacgcgataa acatcttcgc
      961 agtgcggatt ttctcaatac cgcaaaatat ccacaggcaa cattcacctc caccagcgtg
     1021 aagaaagacg gtgacgaact ggatattacc ggcgatctga cgctgaatgg tgtaaccaaa
     1081 cccgtcacgc tggaagcgaa attaattggt cagggcgacg acccatgggg tggtaaacgt
     1141 gcaggcttcg aggccgaagg caaaattaag ctcaaggact tcaatatcaa gacagattta
     1201 ggtccagctt ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa
     1261 gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata tttattctgt tggatcagga
     1321 atgcccagtg ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca
     1381 cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc
     1441 tcactacaac aacctttgta tttttccgca catgctggac actgaataaa cagcaggtgg
     1501 cagccatcat ttttacagtt ggtatggctg tcgcacggcg caccgcactg gtggcaatgc
     1561 gcgataatct catccgatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca
     1621 ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat aattccgccc
     1681 tcgatatgcc agactttgtt gaatccgtta tgtttcatcc aggcactggc cttttcacaa
     1741 cgaatgccgc cggtgcagta catgacgatt tttttatctt tatgtgcctg catcatctcg
     1801 actgcttttg gcagctgctc acggaaggta tctgccggaa tttcgagcgc gttttcaaag
     1861 tgccccactt catactcata gtggttacgc atgtcgataa atagtgcatc gggatcgtca
     1921 agcatggcgt tcacttccgc cgcttgcaga tactcaccaa cattgctggc atcaaagtga
     1981 ggatcgtcaa taccgtcggc aacgatacga tcgcggacct tcatgcgcag tacccagaaa
     2041 gatttcccat catcatccaa cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag
     2101 gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgctg gtacgctgat ttgcgcgtta
     2161 atgccctcat gcgccagata cactcgccca aaaacattca gcgcggtaaa cagctgatat
     2221 aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg
     2281 gttcgcggtt cgctctcagc caacattttg gcttttaacg cgtcgttgga aatgcggttg
     2341 tgtaacactg gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga
     2401 tatagcaatt atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat
     2461 tatttgttat ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg
     2521 ggcgaaaaaa tgcgacaata catacaattg cccgaatagg ttgaaaaaca ggattgatat
     2581 gacgaatcta cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt
     2641 gggtattggc gtactttggt tagtcgtgca attgccctac ccggttatct accgcctcgg
     2701 ttgtggatta ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg
     2761 caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa
     2821 gaatttcgaa tccgttggca tgggactgat ggaaactggc atggcgtggt tctggccgga
     2881 ccgccgaatc gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc
     2941 gcaaaaacgc ggcatcctgt tagttggcat ccattttctg acactggagc tgggtgcgcg
     3001 gcagtttggt atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat
     3061 tgactggcta caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga
     3121 tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg caccggatca
     3181 tgattacggc ccgcgctcaa gcgttttcgt ccctttattt gccgttgagc aggctgcgac
     3241 cacgaccgga acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc
     3301 acgccgtaag ccagatggca aagggtatca attgattatg ctgccgccag agtgttctcc
     3361 gccgctggat gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat
     3421 catgatggca ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gcccggaagg
     3481 cgttccttca cgctattaaa tctcccatgc cggatgcttc agaatggcat ccggcattac
     3541 cacagcaaat ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg
     3601 ggcgcataat tagtgtgctt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca
     3661 gcggattgct atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat
     3721 cgtcgcctgg ctaggctgtt ttcttaccgg tgccgccttc agtctggtaa tgcccttctt
     3781 acccctctac gttgagcagc ttggcgttac cggccactcc gccctgaata tgtggtccgg
     3841 tattgtcttc agcattacat ttttattttc ggccatcgcc tcaccgtttt ggggtggact
     3901 cgccgaccgt aaaggccgaa aactcatgct attacgctct gctctcggca tgggcatcgt
     3961 gatggtgttg atggggctgg cacaaaatat ctggcagttt ttgatccttc gggcgcttct
     4021 tgggttactt ggcggatttg tccccaacgc taatgctctt atcgccacac aagtaccgcg
     4081 taataaaagc ggctgggcac tgggtacgct ctccacaggc ggcgttagcg gtgcgttgct
     4141 cggcccaatg gctggcggcc tgctcgccga tagctacggc ttacgtccgg tattctttat
     4201 taccgccagt gtgctcatac tctgcttttt cgtcaccctg ttctgcatca gagaaaaatt
     4261 ccagccggtc agcaaaaaag agatgctgca catgcgggaa gtggtgacat cacttaaaaa
     4321 cccgaaactg gtactcagcc tgtttgtcac tacgttaatc atccaagtgg cgacgggctc
     4381 aattgccccc attctgacgc tgtatgtccg cgaactggcg ggtaacgtca gtaacgtcgc
     4441 ctttatcagt ggcatgatcg cctcggtgcc aggcgtggcg gctctgctga gtgcaccacg
     4501 actcggcaaa cttggcgatc gaatcggccc cgaaaagatc ctgattacgg cgctgatctt
     4561 ctctgtactg ctgttgatcc caatgtcata cgttcagacg ccattacaac ttgggatttt
     4621 acgttttttg ctcggtgccg ccgatggtgc actactcccc gccgtacaga cactgttggt
     4681 ttacaactcg agcaaccaaa tcgccggacg tatcttcagc tataaccaat cgtttcgtga
     4741 tattggcaac gttaccggac cattgatggg agcagcgatt tcagcgaact acggtttcag
     4801 agcggtattt ctcgtcaccg ctggcgtagt gttattcaac gcagtctatt catggaacag
     4861 tctacgtcgt cgtcgaatac cccaggtatc gaactgattt ttcgcctttc atacttgcaa
     4921 aagcggagaa tcagctatcc ttttccctga aacctcatca actcaaaggg agaatcgtga
     4981 tgaccatgta cgcaacgctt gaagaagcca ttgacgctgc acgcgaagaa tttcttgcag
     5041 acaaccccgg catcgacgcc gaagacgcga atgtgcaaca gttcaatgcc caaaaatacg
     5101 ttttgcagga cggcgacatc atgtggcaag ttgagttttt tgccgacgaa ggggaagaag
     5161 gtgaatgttt acctatgctt agcggtgaag ccgcgcaaag tgtttttgat ggcgactatg
     5221 atgagataga gatccgccag gagtggcagg aagagaatac attacatgaa tgggacgagg
     5281 gggaatttca gcttgagcca ccgctggata ccgaggaagg acgcgcagca gctgatgagt
     5341 gggatgaacg ttaatcactc atacgggcca tgatgaatgt cgatcggcag cagtaaagta
     5401 tcaaaaacca gggagaatgg cagatcaagg attgtgacat aacgccaggc ggagtcacgc
     5461 acatcccatt gcacaccggg ataatattgg ttgccatgcc cctgccccgg tatagtgcgg
     5521 ctaatgatgc tgccacagcc actcagcaag gtcaccatga tgctcaccac aattaaacgc
     5581 atttgatacc tcttccgtaa aaaaataccg gcataacgcc ggtattttta ttggctcgtt
     5641 gtttttgcca gatgcgacgt gaacgcctta cctaacctac aaaatcttgc aaaatcaata
     5701 aattgcagga acgatgtagg cctgataagc gtagcgcatc aggcaactac gtttttattg
     5761 cgaagccgca tccggtttac gcaatgccag tggattgagc tttatccctt cgtaataact
     5821 cacccatgaa gaatatctct caggggaatt ccagacgcgg aaatgcagac gggccatcgt
     5881 caccggatcg cttagcagca caaggcgacg atcgcgattc agcttctctg gcgtctcgtt
     5941 cagcgcctgt tcaacgtggc ggtcacgggc gatttccagc accttactgg cgcggtgacg
     6001 cgcggtcgcc attgcggtgg ccagagcgtt aaatgacggg ttaaacactg cgtgcataaa
     6061 accatcatca agggagcgtt gacggttcat ctcaaggaac cgatcggtat caaccagtac
     6121 ctgcggcggt gaatactctt ccgggatcag gaacagtttc cagcgtttgg tgcgcagacc
     6181 aacggtggca cggctggaaa tcaccgaaac aaacggtgac aggatcaacg agaagacaat
     6241 cggtgccagc cagaacagga aacgcagatc caaccacgcc atcccaacag cccacactaa
     6301 ccctaacagc agctgtgagc cgtggcgttt gaacgcttca ccccaggaag tggagtcatc
     6361 atcacgctgc ggtgaattcc acaccacttc ccagccaagg aacgcgctga cgacgaagac
     6421 cgtatggaac agcatgcgta ccggagccag cagcacggaa aacagcactt ccagcagcaa
     6481 cgataatgta acgcgccaga agccgccata ttctttcgtt cctttgcacc aaataagcaa
     6541 aatgctcaat agctttggca ggaacaacag caccatggtc gaagcaaaaa gtgcaatcgc
     6601 cagctcagga cgccactgcg gccacaccgg gaacaactgc cgtggttgca ggaagtattg
     6661 cggttcggtt aacgcatgca ctacctgcaa tgcagtagag agcgcgagga acataaacca
     6721 cagcggagcg gagagataag acatcacgcc cgtcaggaac accgcacggt gaaccgggtg
     6781 cataccctta accaggaaca gacggaagtt catcaggtta ccgtggcacc agcggcggtc
     6841 acgttttagc tcatcaagta agttaggcgg taattcttca taagaacctg ggagatcgta
     6901 agcaatccag actccccaac ctgcacggcg catcaacgcc gcttccacga agtcatgtga
     6961 caggattgaa ccggcaaagg aaccttcgcc cggcagcgga gccagtgcgc agtgctcgat
     7021 aaacggtttc acgcggataa tcgcgttatg tccccagtag tgcgactcgc caagttgcca
     7081 gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg gtcgcgaact gctgacagcg
     7141 cgcatacagc gtatccatac cggacgcttt cggcgacgac tgaatgatcc cggcgttcgg
     7201 gttggcttcc atcaagcgca ccaggccgca taaacaatca ccggtcatta ccgagtcagc
     7261 atccagcacc accatgtagc tgtactggct gccccagcga cggcagaagt catcgatatt
     7321 accgctttta cgcttcacgc gacgacggcg gcggcgatag aaaatctgac cttcgccacc
     7381 gacttcagcg ataagctcca tccaggcttt ttgctctgcg acgcagatat ccgggttata
     7441 actgtcactc agaatgtaga catcaaagtg tttggcattc ccggtggctt ttactgattc
     7501 ccacgttgca cgcaggccag caaaaacacg gttcacgtct tcgttacaga taggcatgat
     7561 caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca actgttgacg cagatatact
     7621 gtatttatcg cgaccaataa gcagttgcag gaagcccatt aacgccgtcc agaatccggc
     7681 ggacacccaa cagaacagta ccgcaaagag gatcaggata ccggtttgca gcatataagg
     7741 cagaagctgc ataaaggaaa cccacaaatc ctgaccaacc atatccaaag gattaatcag
     7801 cgcccaaccc tgataaggaa gaatggtctt catataccag gtcgcgacga cagtttgcgc
     7861 gagcgtcagg atcaacagaa tgtaacggcg gatggtaccg acggtacgcc acttttgctc
     7921 gctctcctgc tcttctttgg tcaaacgagc cagatagcgc ggcgtgacat cgcgtccacg
     7981 caggcgatcc cagaaacggc ctaccgggtt ggtacgccac gggtcgggaa acatcgagga
     8041 gcgttttgct tctggcatcg ccttaagctg atcgcgccct tcgtcgtctt taattaactg
     8101 tccatcagca agtgaatctg gccaggcttg ttccagacgc gcctttaccg agccttgcgg
     8161 ggagtcatcc tcccgcgccc aggtgcggtg ttcggcatcc agcgcctgat gaacggcgcg
     8221 gatatcagtc ttcggcaatg ccgctttctc gcttgcggcg atgggcattg cgtcaatgta
     8281 ctcagttgtc ttattcattg gcaggtaact ggtagctcca ggtttcactc aacgtctgat
     8341 cggcattcac cagcgcagct cgcatttcag tggttttctt ggcatctttc actttcacac
     8401 gcatcaccag acgccagcct ttggtaaccg ggttatagcg caccgtgctt tcaactatct
     8461 caccattatc accaatgctg gtttgcgctg tgaccggggt atcctctggc agttttttca
     8521 tctctgcgcc ggtaaaatcg accacaaagg cgatagtacc gtcaggctgg cggatcaggt
     8581 tcgactgctt cacatccccc gttgaacgac gcgtttgttg cacccatgcg ttatctggtg
     8641 catgcagttt gtcttcatca cggctgaagg tgatggtgta tttaaagttc atctctttac
     8701 ccggctccgg cagctgatcc ggcgtccagt aagcgacgat gttatcgtta gtttcatcgt
     8761 tggttggaat ttccaccagc tcaacgctgc ctttgcccca ctcccctttc ggagtcaccc
     8821 atgcgcttgg acggagatcg taacgatcat cgagatcttc aaagcgggag aaatcacgac
     8881 cgcgctgcaa cagaccgaag ccttgcgggt tttccatgga gaagctgctg accgctaaat
     8941 gtttcgggtt attcaacgga cgccagatcc attcgccatt accggcatgg atagagagac
     9001 cgttagagtc gtgcaactcc ggacgatagt tatttgcagg cgacggttgg ttcggcccaa
     9061 acaggaacat actggttaac ggtgcaaccc ccagtttgcc gactttatcg cgcagataga
     9121 ttttcgactg cacatccaca accgtgtcac gccctggcat aactacgaat ttgtaagcac
     9181 ctgtcgcgcg cggcgagtca agcaatgcat aaatggttaa acgtttatca gttggttttg
     9241 gacgctcgat ccagaactct ttgaagcgtg gaaattcttc acccgatggc aaggcggtat
     9301 caattgccag accgcgggca gaaaggccat aaacctgacc tgcaccaatc acgcggaaat
     9361 agctggcccc gagcatgctg acgatttcat cgtttttatc tttgctgttg ctcgggtaaa
     9421 gcactttaaa accggcaaaa ccaaggtctt ttaccgtgtc tttgtcatgc tgaacatcgc
     9481 cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac tgcggtggca gtcacttcat
     9541 ttattttgac cggggtatcg aagtacatcc cctgatggta gaactcgagt ttgaatgggg
     9601 tcttcagatt gttccagtac gctttgtcat gattaaactg gatctgctga tagtccgcgt
     9661 atttcatatc gcggaaaacg gagggcaagt tgcttttggg cgcctcatag cctttcccgg
     9721 ctaaggattg agcttgcttt gcgacatcat caatactgaa agcccagctt gaagatgtat
     9781 acagggttaa cattactgca gcactcaacc aacgcatttt catcatttgt agtttatgtt
     9841 tcataattag taagcacttc cccctttgtg tgcttatatc gatccgatct attttaatgg
     9901 ataaccagcc attccgacaa cagaatcccg gtatcgttca gcacgctggc tcatggatta
     9961 agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc taaagacaga agcatcgact
    10021 tcgtgtaggg tttgccagca aatttattta tcaaccgtct tacagacgct tccattctgg
    10081 ttagtggtag cgatgatgct atgcataaaa acttgaagcc tgcgcctggc gcggtcaggt
    10141 cggtgtcgct atggcaacaa gcctaaaggc ataacccgac agggataaga cgaaaaatcc
    10201 gagattagtt aaccatatat gaatccagta cccgcgcaac gtgaatattt cctcgactcc
    10261 atccgcgcct ggctgatgtt gttagggatc ccttttcata tttctttaat ctattcgagc
    10321 catacatggc atgtgaatag cgccgaaccg tcattgtggc tgaccctttt taatgacttc
    10381 atccactcgt tccgcatgca ggtatttttc gttatatccg gctacttttc ctacatgctt
    10441 tttttacgct atcccttgaa aaaatggtgg aaagtacgtg tcgaacgtgt aggtatcccg
    10501 atgttaacag ccatccccct actgacattg ccgcaattta ttatgctgca atacgtcaaa
    10561 gggaaagcgg aaagttggcc tgggctgtca ttgtatgaca aatataatac gttagcctgg
    10621 gaattaatat cgcacctgtg gtttttactg gtgttagtgg tcatgacgac gctgtgcgta
    10681 tggatattta aacgcatcag aaataattta gaaaattctg ataaaacgaa taaaaaattc
    10741 tcgatggtaa aactatcgct gatttttttg tgcctcggca tcggttatgc ggtaataaga
    10801 agaacgattt ttattgtgta tccgcccatt ctgagtaatg gcatgttcaa ttttattgtc
    10861 atgcaaacgc tattttattt gccgttcttt atcctcggcg cactggcttt cattttccct
    10921 catcttaaag ccttgtttac cacgccgtct cgtggctgta ccctcgctgc agcattggcg
    10981 tttgtcgctt atttactcaa ccagcgctat ggcagtggcg atgcctggat gtacgaaacc
    11041 gagtcggtga tcaccatggt cctcggtctg tggatggtga atgtggtctt ctcctttggc
    11101 caccgtttgc ttaacttcca gtcagcgcgg gtgacttact ttgttaacgc atcgctattt
    11161 atctatctgg ttcaccaccc gttaacgctg tttttcggcg cgtacattac accgcacatc
    11221 acctccaact ggcttggttt tctctgtggc ctgatattcg tagtagggat tgcgataatt
    11281 ctgtatgaaa ttcatttacg catcccatta ctgaagtttt tgttctctgg taaaccggtt
    11341 gttaagcgtg agaacgataa agcaccagcc cgttaagccg cgtttacaat aaccattcca
    11401 cgggcaatat cgacgccagt ctgaccataa cccgcttcca gaaactggtg gcgggttctt
    11461 ttttgagaac aatctctttc tttgcatgac gatcaaccca gttgatccgc ccccacctgt
    11521 ccagacggag ctgccaggcc gcatcatact ggctctgaat aaagcgttta tcaattaact
    11581 gtgccagcgt ttcgctctct atcacgaagc ccatttcagt attgagcaat gtcgaacgtg
    11641 gatcgaaatt gaaagagccg ataaacaccg ttttaccatc gatgctaaag gttttagcat
    11701 gcaggctggc tccggaatta ccggttatgc cgcgatcgtg taacgtacta ctttgttcac
    11761 gcgtcggctt gagttcatat aattccacgc catagcggag caattttttg cgccagcgcg
    11821 cgtatccggc atggacgaca gcaacatcgt tagcggcaag agaattggtt aggatcgcaa
    11881 tctttacccc ttttctcacc atccgtaaga gttgcgccac acctgcgcgt gtcggtacaa
    11941 aataggaaga gataatatcg atgcgttcac tgggtgagcc catgatatcg aacaggcgct
    12001 gcggtaacag tgaatgccgt tttgccttgc cctccccttt cgccggatca tcacttaata
    12061 aacgtgtttt cgcccagata agcggcaacg ttccatcaac cagatgattt ataaacggac
    12121 tggattccat tttgcgtaag taacgatgcg tcatggcatc gttatgccag gaggcgggta
    12181 actcgatgcg atccgccatt tcaccttccg ggacatccag cacctgctgt aagggtgaaa
    12241 ccgatttgca ataccagtag cgggcgaaat catcggcaac gtcctctact acgggtccta
    12301 ttgccatgac atctaaatcc gaaaaaagtg gctcttcccc tgccccaaaa taggcatcac
    12361 caatatttcg cccccctacc agcgtcacca cgccatcgac agtgaaactt ttattgtgca
    12421 tacggcgatt gagacgggaa aagtcggtga tataaccaag cggacgcagc aagcgaaacg
    12481 agaaaggatt aaaaagccgg acttcaatgc ggggatggct gtcaagcaag cgtaaaatgt
    12541 cgtcaagtcc gggcgtattg ttgtcgtcca gcaacaaacg gacgcgaacg ccacgctttg
    12601 ctgcggctaa cagggcggaa aacagtaacc gacccgacat atcgtcctgc cagatgtaat
    12661 actgaacatc cagcgtatgc tctgccattt cggccagacg ataccgggcg gcaaacgcat
    12721 ccagactttt ctccagaggg aaaagaccac actgaccggg atgttgcgaa cacagtggca
    12781 gcaccgcgct cgccagccgg ggcaaatcat tcatctcctt gttgggtaag gagtctttcg
    12841 tagaggtggg agttttcttc atcataacag acaaagtata cctgttcggg taaagcgtga
    12901 cgggtaataa attctgaaac ggtttttact gcgatttcag ccgctgccgc acgagggtaa
    12961 ccataaaccc cagtactgat tgcaggaaaa gccactgacg tatagctgtt tgccgccacc
    13021 agtcgcaggc tattgagata ggcatcctgc aaaagctggt cttcgttttg ttcaccacca
    13081 cgccagactg gcccgacggt gtgcactacg gctttagcag gaagatcgcc tgcaagcgta
    13141 ataacggcat gccccgtagg gcaatcgccc tgctgttgcc taacttttaa acaggcatcc
    13201 agcagggccg gacccgctgc gcgatgaatg gccccatcga cgccgccgcc tcccattaat
    13261 gacggattag ccgcattcac aatcacatca acggccagtt tggtaatatc accctgcaca
    13321 acatgaatac gcgttttcat aacgcctcct tacaccttct tgtttcttaa gtgtacagca
    13381 ggctttttgg ttaaatcgca tttttctgct cagcaacgtg agttgttcag aaacttagtt
    13441 atattcgatg ttcattacag ccagcttttt ctgtttatca agatactgca cttttactga
    13501 ggctacttgc gagtttttaa cattgccgct cattaacgct ttgctggaat aggtttttcc
    13561 cggaacagaa ccattctgtg ggcagttcat ttcaacgggt gatgactggg tgctgacgtc
    13621 acatgccggt tctacaattt ggccataaaa atggatcacg cccccttgcg cggtgcttcc
    13681 tgcgatggca ataaaaggaa aaaacaaact gccgagcata agagagttta aaaaaggtct
    13741 gaacatttat tctatttcct caatgattag tcatccttga gggttgtgtt atccatactt
    13801 tcaggttggc tgattcgcag cagaccattc tctccagatt catcttatgc tcgatatttc
    13861 aacaaattaa gacttttctg aagagggcgg ccattgttgt gataaatgaa gtgactgtcc
    13921 atcagaaaca gtaacaacta ttttcacccg atcgtccggg gaaatattta aactcaactt
    13981 cgtcaaagca atgggttgat tagcaggcaa tgaaagggtc ttttcttgct tcgtctgact
    14041 ttgccctgaa ctgccttcgc gcagggacaa tatttgtact ctgcacagac aagattgagt
    14101 aagagtgact tcaggaataa tggtatacac atccccttgc tgggtcgtat taaaggttat
    14161 ctgactggaa agtgccgcaa ggagtaataa cgcattcata ttcttctccc gaaaaaaaac
    14221 agggcttgcg ccctgtttct gtaatacaaa tgatgtatta gtactgatga gcggtcgcgt
    14281 tgttaccaaa gccaacctga gtcacgttga cggaggagtt agatgcagtc tggtcaactg
    14341 cagcaccgtt gccaccaccg aactgtttaa ccgtcatttc agaatttttg ccgttccact
    14401 gatcaagagt agcgctgtta ccgaagccac gttgggtcag atcgattgag ctgtcatccg
    14461 agccctgacc aacatctgca ccattaccgc cgccatgctg ggtaatagtc aagtcagagt
    14521 tacgggcatc agtttgcaga gcaagtgcag agttaccgcc accgtactgg taaatgttca
    14581 gctcagaatt tgggccgcta ttattaccgc caccaccgtg gttaccgccg ccgccgtact
    14641 gaggaacaac acctgccaga gcgctaccgg agaatacgat tgctgcaatt gctgctactt
    14701 ttaaaagttt catgtaaaac ccccatcgga ttgatttaaa agtcgaatgg aaattaacgt
    14761 tgtgtcacgc gaatagccat ttgcgactgt ctctgcacta caattgccgt tttttgagta
    14821 ccatactgtg taatatttgc tttattacca gaacctttct ggataatcat cgcagtatta
    14881 ccataagcac cttgcgaaat actggcatcg ttggcactgc ccgcctgatc aatatatgca
    14941 aggttataat ctcctgtctg gtcaatcttt gcccggttgc tactaccttc ttgcgcaaca
    15001 accgccaaaa gttttgagcc tccctgccgt aactgagcac tattattagt cccagcttga
    15061 ccaattatgg ctgcctgatt aaatgaagac ttactcaatt catttaccgc gaagttatat
    15121 tctgaattag ctaaatcata acctgctgcg gctgcaatcc caggcgcacc cagtattgtt
    15181 aacatcataa ataacaattt gtttttcatg ttgtcaccct ggacctggtc gtacatttaa
    15241 gaaattaaat catttcaact tggttgttaa cgcaacctgt attttgttaa cgctgcgtta
    15301 cgatggaaag tatgtctgcg gaaatatttt taataactca cccgcgcgtt gtatttttct
    15361 ttttttgttc ataccaaagt attaaaagcc acgtaaatac gctgttatct acgcaaaaaa
    15421 tattttgttt tcttttaaat ctccgtttcc gctatcaaaa atcaccagac agtcattctt
    15481 cttgcccgtc gctgattgct g
//
LOCUS       DAZGBC010000114        15443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGBC010000114 DAZGBC010000000
VERSION     DAZGBC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.115"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            134..1111
                     /gene="ybeQ"
                     /locus_tag="ACHK1J_003463"
     CDS             134..1111
                     /gene="ybeQ"
                     /locus_tag="ACHK1J_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO7912690.1"
                     /translation="MIFTSSCCDNLSIDKIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1181..1663)
                     /gene="ybeL"
                     /locus_tag="ACHK1J_003464"
     CDS             complement(1181..1663)
                     /gene="ybeL"
                     /locus_tag="ACHK1J_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO7912691.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1899..4481
                     /gene="leuS"
                     /locus_tag="ACHK1J_003465"
     CDS             1899..4481
                     /gene="leuS"
                     /locus_tag="ACHK1J_003465"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO7912692.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDSEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4496..5077
                     /gene="lptE"
                     /locus_tag="ACHK1J_003466"
     CDS             4496..5077
                     /gene="lptE"
                     /locus_tag="ACHK1J_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO7912693.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5077..6108
                     /gene="holA"
                     /locus_tag="ACHK1J_003467"
     CDS             5077..6108
                     /gene="holA"
                     /locus_tag="ACHK1J_003467"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO7912694.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6110..6751
                     /gene="nadD"
                     /locus_tag="ACHK1J_003468"
     CDS             6110..6751
                     /gene="nadD"
                     /locus_tag="ACHK1J_003468"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO7912695.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            6775..7386
                     /gene="cobC"
                     /locus_tag="ACHK1J_003469"
     CDS             6775..7386
                     /gene="cobC"
                     /locus_tag="ACHK1J_003469"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO7912696.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7646..7963
                     /gene="rsfS"
                     /locus_tag="ACHK1J_003470"
     CDS             7646..7963
                     /gene="rsfS"
                     /locus_tag="ACHK1J_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO7912697.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7967..8434
                     /gene="rlmH"
                     /locus_tag="ACHK1J_003471"
     CDS             7967..8434
                     /gene="rlmH"
                     /locus_tag="ACHK1J_003471"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO7912698.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8465..10366
                     /gene="mrdA"
                     /locus_tag="ACHK1J_003472"
     CDS             8465..10366
                     /gene="mrdA"
                     /locus_tag="ACHK1J_003472"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO7912699.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10369..11481
                     /gene="mrdB"
                     /locus_tag="ACHK1J_003473"
                     /gene_synonym="rodA"
     CDS             10369..11481
                     /gene="mrdB"
                     /locus_tag="ACHK1J_003473"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO7912700.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11492..12580
                     /gene="rlpA"
                     /locus_tag="ACHK1J_003474"
     CDS             11492..12580
                     /gene="rlpA"
                     /locus_tag="ACHK1J_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO7912701.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLSATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLRQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12720..13931
                     /gene="dacA"
                     /locus_tag="ACHK1J_003475"
     CDS             12720..13931
                     /gene="dacA"
                     /locus_tag="ACHK1J_003475"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO7912702.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            14042..14305
                     /gene="ybeD"
                     /locus_tag="ACHK1J_003476"
     CDS             14042..14305
                     /gene="ybeD"
                     /locus_tag="ACHK1J_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO7912703.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14406..15047
                     /gene="lipB"
                     /locus_tag="ACHK1J_003477"
     CDS             14406..15047
                     /gene="lipB"
                     /locus_tag="ACHK1J_003477"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO7912704.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15307..>15443
                     /locus_tag="ACHK1J_003478"
     CDS             15307..>15443
                     /locus_tag="ACHK1J_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7912705.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     G"
BASE COUNT         3857 a         3893 c         4000 g         3693 t
ORIGIN      
        1 atgtaaacta cattacgagg ctcatttatt tatacttttc tgacacaaca taatacgcat
       61 aaaataagac ttacgcaaaa acggaacaca taaaaatata atatgtttta tctgcaacca
      121 taaggaaatg ataatgattt ttacgtcaag ttgctgcgat aatttatcaa tagataagat
      181 tatcgaacgt gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa
      241 tcgcgatagc gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc
      301 cgctgagcaa ggtcattgtg aagcacaata ctccttaggg caaaagtata ccgaggataa
      361 aagccgtcat aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg
      421 ccatactttc gcttccaacg cccttggctg gatactggat cgtggagaag accccaacta
      481 taaagaagcg gttgcctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa
      541 taatcttggg tggatgtaca gaaatggcaa cggagtcgca caagactata cgctggcatt
      601 tttctggtac aaacaagctg cattacaagg ccatagtgac gcgcaaaaca atctggccga
      661 tctttatgaa gacggaaaag gcgttgctca aaacgagaca ctcgccgcat tctggtattt
      721 gaaaagcgca cagcagggta atcggcatgc tcagtttcaa attgcatggg attataacgc
      781 tggcgaaggg gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc
      841 tcaggggagc gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt
      901 tgagaaagat tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc
      961 cactgcctgg tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct
     1021 ccgacaggct ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca
     1081 ggaaattcgt gagactgaag atttactgta gagataaaaa caagggccgg ggaatttcat
     1141 tcggcccata ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc
     1201 tctggaactg gtcatgacca catttcgggc atagcgtcag cacttccggt gtgtagatcg
     1261 ggagatggaa gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt
     1321 gataaacccc atgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat
     1381 cggtgatgtc tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt
     1441 cagattcttc tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga
     1501 cagctcgcgt cagctcatcg acctcggttc gcgttaactc ccctgttttt attacgcgct
     1561 cgcgcgcctg ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca
     1621 gtgacgcaac cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt
     1681 cgttaactga ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa
     1741 ctctgtaaat taatgtatga ataatccggg catcatttca acgtcgagtg cgcgaaaggc
     1801 tgttttgacg cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tctaacgcta
     1861 cttttgtagc cgtaatgaaa acaggaccac tggctgccat gcaagagcaa taccgcccgg
     1921 aagagataga atccaaagta cagcttcatt gggatgagaa gcgcacattt gaagtaaccg
     1981 aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct tctggtcgac
     2041 tacacatggg ccacgtacgt aactacacca tcggtgacgt gatcgcccgc taccagcgta
     2101 tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc gtttggtctg cctgcggaag
     2161 gcgcggcggt gaaaaacaac accgctccgg caccgtggac gtacgacaac atcgcgtata
     2221 tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag ctggcaacct
     2281 gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat aaaaaaggcc
     2341 tggtatataa gaagacttct gcggtcaact ggtgcccgaa cgaccagacc gtactggcga
     2401 acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgaa cgtaaagaga
     2461 tcccgcagtg gtttatcaaa atcactgctt acgctgacga gctgctcaac gatctggata
     2521 aactggatca ctggccagac accgttaaaa ccatgcagcg taactggatc ggtcgttccg
     2581 aaggcgtgga gatcaccttc aacgttaacg actatgacaa cacgctgacc gtttacacta
     2641 cccgcccgga cacctttatg ggttgtacct acctggcggt agctgcgggt catccgctgg
     2701 cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt tattgacgaa tgccgtaaca
     2761 ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat actggcttta
     2821 aagcggttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga
     2881 tggagtacgg cacgggcgca gttatggcgg ttccggggca cgaccagcgt gactacgagt
     2941 ttgcctctaa atacggcctg aacatcaaac cggttatcct ggcagctgac ggctctgagc
     3001 cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct ggcgagttca
     3061 acggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact gcgatgggcg
     3121 ttggcgagcg taaagtgaac taccgcctgc gcgactgggg ggtttcccgt cagcgttact
     3181 ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgccg accccggacg
     3241 accagctgcc ggtgatcctg ccggaagatg tggtaatgga cggcattacc agcccgatta
     3301 aagcagatcc ggagtgggcg aaaactaccg ttaacggtat gccagcactg cgtgaaaccg
     3361 acactttcga cacctttatg gagtcctcct ggtactatgc acgctacact tgcccgcagt
     3421 acaaagaagg tatgctggat tccgaagcgg ctaactactg gctgccggtg gatatctaca
     3481 ttggtggtat cgaacacgcc attatgcact tgctctactt ccgcttcttc cacaaactga
     3541 tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa acagttgctg tgtcagggta
     3601 tggtgctggc agatgccttc tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc
     3661 cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg
     3721 caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag aacaacggta
     3781 tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg tttatgatgt
     3841 ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggcgtggaa ggggctaacc
     3901 gcttcctgaa acgtgtctgg aaactggtgt acgagcatac tgcgaaaggt gatgttgcgg
     3961 cactgaacgt tgatgcgctg actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa
     4021 cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg
     4081 cgattatgga gctgatgaac aaactggcga aagcaccgac cgatagcgag caggatcgcg
     4141 ccctgatgca ggaagcgctg ctggccgttg tccgtatgct taacccgttc accccgcaca
     4201 tctgcttcac gctgtggcag gaactgaaag gcgaaggcga tatcgacaac gcgccgtggc
     4261 cggttgctga cgaaaaagcg atggtggaag actccacgct ggtcgtggtg caggttaacg
     4321 gtaaagtccg tgccaaaatc accgttccgg tggacgcaac ggaagaacag gttcgcgaac
     4381 gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttactgta cgtaaagtga
     4441 tttacgtacc aggtaaactc ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg
     4501 atatctggca acattgttgt tatctctggc ggtgttaatc accgccgggt gtggctggca
     4561 tctgcgtgat accacgcagg ttccttccac tatgaaggtc atgatcctgg actcaggtga
     4621 tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt
     4681 gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttaggta aagtgagcat
     4741 cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc agatgatcat
     4801 gacggtgaat gcgaccgtgt tgatccccgg ccgtgacatc tacccgatta gcgccaaggt
     4861 cttccgttcg ttcttcgata acccgcaaat ggcgttagcg aaagataacg aacaagacat
     4921 gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc tgccaagcat
     4981 ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg actacaacgg atactccggc
     5041 aacgcctgca cgcgtctcca ccacgctggg taactgatga ttcggttgta cccggaacaa
     5101 ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg taacgatcct
     5161 ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag ctgcggcaca aggattcgaa
     5221 gaacaccaca ctttttccat tgatcccaac actgactgga atgcgatctt ttcgttatgc
     5281 caggccatga gtctgtttgc cagtcgacaa acgctattgc tgttgttacc agaaaacgga
     5341 ccgaacgcgg cgatcaatga acaacttctc acactcaccg gacttctgca tgacgacctg
     5401 ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc ctggtttact
     5461 gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc tcagcttccc
     5521 cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac tggatgacgc ggcgaatcag
     5581 gtgctctgct actgttatga aggtaacctg ctggcgctgg cccaggcact ggagcgttta
     5641 tcgctgctct ggccagacgg caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat
     5701 gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc
     5761 gcgttgcata ttcttcagca actgcgtctg gaaggcagcg agccggttat tttgttgcgc
     5821 acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc ccatacgcca
     5881 ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat gatgggcgag
     5941 gcgttaaatc gcttaagtca gccgcagtta cgtcaggccg tgcaactcct gacacgaacg
     6001 gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg cagagctgga agggttatct
     6061 cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata tgaaatcttt
     6121 acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctaa aacccgtgga
     6181 aacgctggcg aatttgattg gtctgacgcg agtcacaatc atccctaata atgttcctcc
     6241 gcatcgtccc cagccggaag cgaacagcgt acagcgtaaa cacatgcttg aactggcgat
     6301 tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg ccccctctta
     6361 cactgcgcaa acactgaaag agtggcggca ggaacaaggg ccggacgtgc cgctggcgtt
     6421 tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg aaacgatact
     6481 cgacaatgca catttgatcg tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc
     6541 gcaataccag caatggctgg aagatcattt gacacataac ccggaagatc ttcaccttca
     6601 gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg cgaccatcat
     6661 ccgcgaacgt ttgcaaaacg gtgaatcatg tgatgattta ttgccggaac cggtactgac
     6721 ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg catgatgcga
     6781 ctgtggttaa ttcgtcatgg cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat
     6841 gcgcccacac ccctgaccgc acgcggtatt gagcaagcgc aaaatctgca tacactgcta
     6901 gatgatgttt cctttgacct ggttttatgc agtgaactgg aacgggcaca gcataccgcg
     6961 cgactggttc tcagtgaccg ccagctcccc gttcatatca tccctgaact caacgaaatg
     7021 ttttttggcg actgggagat gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac
     7081 tatagcgcgt ggtgcaatga ctggcagcat gctatcccca cgaacggtga aggatttcag
     7141 gcattttcgc aacgtgtgga acgctttatc gcaaggctta gtgaatatca gcactatcag
     7201 aatattttaa tcgtcagcca tcagggcgta ctgagtctgt taattgctcg tttaattggc
     7261 atgcctgccg agtctatgtg gcattttcgc gttgaccagg ggtgttggag cgccattgat
     7321 atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaat
     7381 gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacagc cccgggcagg
     7441 ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa attgttttac
     7501 aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat
     7561 tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc
     7621 tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga
     7681 caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcagg gcaaatccag
     7741 catcaccgac tgcatgatca tctgtacggg tacgtccagc cgtcatgtta tgtccattgc
     7801 tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga
     7861 aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga
     7921 agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact
     7981 tgtcgccgta ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg
     8041 tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa aacgcggcaa
     8101 gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg
     8161 caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc cgcagttagc
     8221 cgctgagctg gaacgctgga agctggatgg tcgcgacgtc agtctactga ttggcgggcc
     8281 tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag agctggtcgc tgtcggcgct
     8341 taccctcccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag
     8401 catcaccacc aaccatcctt atcaccgtga gtgataaggg agctttgagt agaaaacgca
     8461 gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg
     8521 ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct
     8581 gtataatctg caaattgttc gctttaccga ctaccagacc cgctctaatg aaaaccgcat
     8641 taagctggtg cctatcgcgc ccagccgcgg cattatctat gaccgtaacg gtatccctct
     8701 ggccctcaac cgcactatct accagataga aatgatgccg gagaaagtcg ataacgtgca
     8761 gcaaacgctg gacgctttgc gcagcgtggt agatctgacc gatgacgata ttgctgcatt
     8821 ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa ctaacctgac
     8881 cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa
     8941 aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca tcggctatgt
     9001 gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta
     9061 tgcggcaacg catgatatcg gtaagctggg cattgagcgt tactatgaag acgtgctgca
     9121 cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt
     9181 aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc tcaaactcca
     9241 gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggtagtca ccgatccgcg
     9301 tacaggtggg gtgctggcgc tggtttccac gcctagttat gacccaaacc tgtttgttga
     9361 cggtatctcc agcaaagatt attccgcctt gctgaatgac ccgaatacac cgctggtgaa
     9421 ccgcgccaca cagggggttt atcctcccgc gtctacagtg aaaccctatg tggcggtttc
     9481 ggcattgagc gccggggtga tcacgcgcaa tacgacgctg tttgacccag gctggtggca
     9541 actaccaggg tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg gacgtctgaa
     9601 tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat
     9661 ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt acaccggtat
     9721 cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac agaaacgctt
     9781 taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggtcagg gttactggac
     9841 agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa
     9901 ggttccgcat ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacaacc
     9961 gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga aagacggtat
    10021 gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tactttgcca gcgcaccgta
    10081 caaaattgcg gcgaaatccg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta
    10141 taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc
    10201 atacaacaat ccgcaagtgg ctgtcgccat gattctggaa aacggcggcg cgggtccggc
    10261 ggttggtacg ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga
    10321 tctgcctgcg gaaaatccag cggttgccgc agcggaggac cattaatcat gacggataat
    10381 ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatttta
    10441 ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc
    10501 atgatggagc gtaaaatcgg ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg
    10561 caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt
    10621 ttgctggtgg cggtagatgc tttcggtgcc atctctaaag gcgctcaacg ctggctggac
    10681 ctcggtattg ttcgttttca gccgtcggaa attgccaaaa tagccgtacc actgatggtt
    10741 gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga agaacactgg catcgcgctg
    10801 gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacctggg aacatcaatc
    10861 ctcgttgcgc tttccggtct gtttgtactg ttcctctctg gccttagctg gcgtctgatt
    10921 ggcgtcgcag tagtgctggt agcggcgttc attccgattc tgtggttctt cctgatgcat
    10981 gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgcg
    11041 ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc
    11101 tggctgcacg gcactcagtc acagcttgaa tttctccccg aacgccatac tgactttatc
    11161 ttcgcggtac tggcggaaga gctgggatta gtgggcattc tgattctgct cgctctctac
    11221 attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc
    11281 gtcatggctg gtggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg
    11341 gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg
    11401 gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa
    11461 atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat
    11521 cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga cggtaagtgt
    11581 accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcgg acccgcgttt
    11641 cgaaccactg agcgcgacgg caaatcagga ttaccagcgc gacggtaaaa gctacaaaat
    11701 cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg atgccgaacc
    11761 aggcagtaac ctgacggcct ctggcgaagc tttcgatccg acacagctga cggcggccca
    11821 tccaacgctt ccgatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat
    11881 cgtggtgcgc attaatgatc gcggtcctta cggcaacgac cgcgttattt cgctttctcg
    11941 cgcagcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat
    12001 tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca
    12061 gacttacgcc ctgcctgcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc
    12121 tggcccgcag ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac
    12181 cggcgcgccg gtaaccagca gcggtttcct cggcgcacca acgaccttag cgcctggtgt
    12241 actggaaggc agcgaaccga cgcctgcgcc acagcccgtt gttacagctc cgtcgacaac
    12301 gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa
    12361 ctttatggtg caggtcgggg ccgttagcga tcaggctcgt gcgcaacagt accaacagca
    12421 actgagacag aagttcggcg tccccggtcg cgtaactcaa aatggcgcag tctggcggat
    12481 ccagcttggc ccattcgcca gcaaagcaga agccagtacc ttgcagcaac gtttgcaaac
    12541 cgaagcccaa ttacagtcat ttattaccac cgcgcagtag cgtaaagcag gcatctgatg
    12601 tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag
    12661 tgcatttgct atagtagggc acttttttta attccatcac ggatgtcgta gttcagacca
    12721 tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg gctctttgca
    12781 cagcctttat ctctgctgca catgccgatg acctgaatat caaaactatg atcccgggtg
    12841 taccgcagat cgatgcggag tcctacatcc tgattgacta taactccggc aaagtgctcg
    12901 ccgaacagaa cgcagatgtc cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt
    12961 acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta gtcactatcg
    13021 gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca
    13081 aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg caatcgggta
    13141 acgatgcttg tgtcgccatg gccgattttg ccgctggtag ccaggatgct tttgttggct
    13201 tgatgaacag ctacgttaac gccctgggcc tgaaaaacac ccacttccag acggtacatg
    13261 gtctggatgc tgatggtcag tacagctccg cgcgagatat ggcgctgatc ggccaggcat
    13321 tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt acgtttaacg
    13381 gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat gtcgacggca
    13441 tcaaaaccgg acacactgac aaagcaggtt acaaccttgt tgcttctgcg actgaaggcc
    13501 agatgcgctt gatttctgcg gtgatgggcg gacgtacttt taaaggccgt gaagccgaaa
    13561 gtaaaaaact gctaacctgg ggcttccgtt tctttgaaac cgttaaccca ctgaaagtag
    13621 gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct tcgttagggg
    13681 ttgataaaga cgtgtacctg accattccgc gtggccgcat gaaagatctg aaagccagct
    13741 atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta
    13801 tcaacttcca gcttgatggc aaaacgatcg agcaacgccc gctggttgtg ttgcaagaaa
    13861 tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg ttccatcact
    13921 ggtttggtta aaaattgaac acttgaaagt gtaatttccg tccccatata ctaagcatca
    13981 gtaaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg ccggagctga
    14041 catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt acaaagttat
    14101 ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc gccatgcgcc
    14161 aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact cggtatctat
    14221 cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat
    14281 cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggcacct ggtcgggtaa
    14341 ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc cacggagatg
    14401 ccgttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct tacgagccaa
    14461 tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt gatgaaatct
    14521 ggctggtcga gcactatccg gtattcaccc aagggcaggc aggaaaggcg gagcacattt
    14581 taatgcctgg tgatattccg gtgattcaga gcgatcgcgg tgggcaggtg acttatcacg
    14641 ggccggggca acaggtgatg tatgtattgc ttaacctgaa acgccgtaaa ctcggtgtgc
    14701 gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa ctgggtatag
    14761 aagcgcatcc tcgggctgac gcgcctggtg tctatgttgg ggaaaagaaa atttgctcac
    14821 tgggtttacg tattcgacgc ggttgttcat tccacggtct ggcattaaac gtcaatatgg
    14881 atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa atggctaaaa
    14941 tatcacaatg gaaacccgaa gcgacgacta ataatattgc accacgttta ctggaaaata
    15001 ttttagcgct actaaacaat cctgacttcg aatatattac cgcttaattt catacctcaa
    15061 tggcccaatt tacactgggt cattactcat aacacctgct aatgctttac atctccttcg
    15121 cgtttgtata ttttctttat cacttgcaga aatgcgatgc tacgccttgc tccgtaactc
    15181 accccattga ttaacttatt gaaatagttg attatgatga gcaaaagcgt tccgttccaa
    15241 ttagacataa gattattgta atttttttaa atattatttt tccatgaata aaattggagt
    15301 aagtgcgtgg atagtaataa tcaaatcgaa ccctgtttaa gcagaaagtc gtcagaaggt
    15361 aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac tatttttgaa
    15421 gctgtatatg tacataaagg gat
//
LOCUS       DAZGBC010000115        15234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGBC010000115 DAZGBC010000000
VERSION     DAZGBC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.116"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            295..1701
                     /gene="chiP"
                     /locus_tag="ACHK1J_003479"
     CDS             295..1701
                     /gene="chiP"
                     /locus_tag="ACHK1J_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO7912706.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            1751..2077
                     /gene="chiQ"
                     /locus_tag="ACHK1J_003480"
     CDS             1751..2077
                     /gene="chiQ"
                     /locus_tag="ACHK1J_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO7912707.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(2161..2607)
                     /gene="fur"
                     /locus_tag="ACHK1J_003481"
     CDS             complement(2161..2607)
                     /gene="fur"
                     /locus_tag="ACHK1J_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO7912708.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(2600..2686)
                     /gene="uof"
                     /locus_tag="ACHK1J_003482"
     CDS             complement(2600..2686)
                     /gene="uof"
                     /locus_tag="ACHK1J_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO7912709.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            complement(2896..3426)
                     /gene="fldA"
                     /locus_tag="ACHK1J_003483"
     CDS             complement(2896..3426)
                     /gene="fldA"
                     /locus_tag="ACHK1J_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO7912710.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            complement(3566..3859)
                     /gene="ybfE"
                     /locus_tag="ACHK1J_003484"
     CDS             complement(3566..3859)
                     /gene="ybfE"
                     /locus_tag="ACHK1J_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO7912711.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            complement(3999..4763)
                     /gene="ybfF"
                     /locus_tag="ACHK1J_003485"
     CDS             complement(3999..4763)
                     /gene="ybfF"
                     /locus_tag="ACHK1J_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7912712.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPTMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4948..5493
                     /gene="seqA"
                     /locus_tag="ACHK1J_003486"
     CDS             4948..5493
                     /gene="seqA"
                     /locus_tag="ACHK1J_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO7912713.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5519..7159
                     /gene="pgm"
                     /locus_tag="ACHK1J_003487"
     CDS             5519..7159
                     /gene="pgm"
                     /locus_tag="ACHK1J_003487"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO7912714.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(7334..8011)
                     /gene="kdpE"
                     /locus_tag="ACHK1J_003488"
     CDS             complement(7334..8011)
                     /gene="kdpE"
                     /locus_tag="ACHK1J_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO7912715.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8008..10692)
                     /gene="kdpD"
                     /locus_tag="ACHK1J_003489"
     CDS             complement(8008..10692)
                     /gene="kdpD"
                     /locus_tag="ACHK1J_003489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO7912716.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLLVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            complement(10685..11257)
                     /gene="kdpC"
                     /locus_tag="ACHK1J_003490"
     CDS             complement(10685..11257)
                     /gene="kdpC"
                     /locus_tag="ACHK1J_003490"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO7912717.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            complement(11266..13314)
                     /gene="kdpB"
                     /locus_tag="ACHK1J_003491"
     CDS             complement(11266..13314)
                     /gene="kdpB"
                     /locus_tag="ACHK1J_003491"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO7912718.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTCILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            complement(13337..15010)
                     /gene="kdpA"
                     /locus_tag="ACHK1J_003492"
     CDS             complement(13337..15010)
                     /gene="kdpA"
                     /locus_tag="ACHK1J_003492"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO7912719.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPFGSGLARLINDIPLPGTAGVER
                     VLFSALGVSDREMNWKQYLSAILCLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFSLIRAFTRQSM
                     STLGNAWVDLLRITLWVLAPVALLIALFFIQQGALQNFQPYQAVTTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            complement(15010..15099)
                     /gene="kdpF"
                     /locus_tag="ACHK1J_003493"
     CDS             complement(15010..15099)
                     /gene="kdpF"
                     /locus_tag="ACHK1J_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO7912720.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
BASE COUNT         3725 a         4161 c         3944 g         3404 t
ORIGIN      
        1 aacaaaacat aattaaataa aatgtaaccg ctttcatctt gctggaaatt cacgctttta
       61 ttcttctgca agcctttcaa ccgcaaactt aagccttgta acaaaaatca tcaaaatatg
      121 tgcggttgct catgttctta cattctggtt acagaaagag attgataatt cgcgccgcga
      181 aaaatagtct gttcctgtag tcagcgagac ttttctcaac gctacttttt taatttttat
      241 tttttcgctg ttcacctttg gtgcagcaat ttatacgtca aagaggatta acccatgcgt
      301 acgtttagtg gcaaacgtag tacgctggcg ctggctatcg ccggtgttac agcaatgtcg
      361 ggctttatgg caatgccgga ggctcgcgcc gaaggattca tcgacgattc aaccttaacc
      421 ggcggtatct attactggca gcgtgaacgc gaccgtaaag atgttaccga cggcgacaaa
      481 tacaaaacca acctttctca ctccacctgg aatgccaacc tcgattttca gtctggttat
      541 gctgctgata tgttcggcct tgatattgcc gcgtttacgg cgattgaaat ggcggaaaac
      601 ggcgacagct ctcacccgaa cgaaatcgcg ttttcaaaaa gtaataaagc ctatgacgaa
      661 gactggtccg gcgataaaag cggtataagc ctgtataaag cagcggccaa atttaaatac
      721 ggtccggttt gggcgagggc aggttatatt cagccaaccg gtcagacgct gttagcgccg
      781 cactggagct ttatgccagg tacctatcag ggtgcggaag ccggagcgaa ttttgattac
      841 ggcgatgccg gtgcgttgag tttctcctac atgtggacca acgaatacaa agcaccgtgg
      901 catctggaaa tggatgagtt ttatcagaac gataaaacta ccaaagttga ttatctgcac
      961 tccatcggtg cgaaatacga cttcaaaaat aacttcgtac tggaagcggc atttggtcag
     1021 gcggaagggt atatcgatca atactttgcc aaagccagct acaaatttga tatcgccggt
     1081 agcccgttaa ccaccagcta ccagttctac ggtacgcgcg ataaagttga cgatcgcagc
     1141 gtcaacgatc tttatgacgg caccgcctgg ctgcaagcgt tgacctttgg ttaccgggcg
     1201 gctgacgtag tggatttgcg cctcgaaggc acctgggtta aagctgacgg tcagcaggga
     1261 tacttcctgc aacgtatgac tccaacctac gcttcctcga acggtcgcct ggatatctgg
     1321 tgggataacc gttctgactt caacgccaac ggcgaaaaag cggtcttctt cggtgcgatg
     1381 tatgacctga aaaactggaa tcttccaggc ttcgccatcg gcgcttccta cgtttacgca
     1441 tgggatgcta aacctgcgac ctggcagagc aatccggatg cgtactacga caaaaaccgg
     1501 actattgaag agtctgccta cagcctggat gcggtctaca ccattcagga cggtcgcgcc
     1561 aaaggcacga tgttcaaact gcacttcacc gaatacgaca accactccga catcccaagc
     1621 tggggcggcg gttacggtaa catcttccag gatgagcgtg acgtgaaatt tatggtaatc
     1681 gcaccattca ccatcttctg atgcccgacg cggcaggttt tgtgcctgcc gcacgtttga
     1741 ggaatttgct atgaaaaaac tgattctcat cgccatgatg gcatcggggc tggtagcttg
     1801 tgcgcaatca accgcaccac aggaagacag ccgtctgaaa gaggcataca gcgcctgtat
     1861 caacaccgca caaggttcgc cggaaaaaat tgaagcctgc cagagcgtgt taaacgtgct
     1921 gaagaaagag aaacaacatc agcagtttgc cgaccaggaa agtgtccgcg tactggatta
     1981 tcagcagtgt ctacgtgcaa cgcaaaccgg aaatgatcag gcggtgaaag ccgattgtga
     2041 taaggtctgg caggaaattc gcagtaataa caaataagtg agagctgtaa ctctcgcttt
     2101 tcttatttcc cttgcataaa aaagccaacc cgcaggttgg cttttctcgt tcagactggc
     2161 ttatttgcct tcgtgcgcgt gctcatcttc gcggcaatcg ccttcggcac agtgaccgta
     2221 aagatagaga ctgtggttag tcaggcgaat gccatgtttt gcggcaattt cacgctgacg
     2281 cgcttcgatt gaatcatcac taaattcgat aaccttgccg cagtcgaggc agatcaggtg
     2341 atcgtggtga tgttgctgtg tcagttcaaa tacggattta ccgccttcaa aattgtggcg
     2401 ggtgacgata ccagcgtcgt caaactggtt cagtacgcga tataccgtag ccagaccaat
     2461 ttcttcaccc atatcgatca gacgtttgta taaatcttcc gcactgacgt gatggttgtc
     2521 cggctcctga agaacttcca ggatttttaa acgtggaagc gttactttca ggccagcttt
     2581 ctttagggcg gtattgttat cagtcatgcg gaatctgtcc tgttactaag cggttcactt
     2641 cattagaaga agtgacagaa tttgctcttg agataatgcg tatcattata gaattgccac
     2701 gcctaaatga aaaccacaag tccctggcaa atattgttaa taaaaacgtg gcacagctta
     2761 catttacaac ggcaaggcca cttgaacacc gggacattgt acaggtacaa cagcaaaagt
     2821 tacaaatttg tagcaattat tttgattggc attatctatt aatacggcgt agacatgagt
     2881 ctacgccgca tcacatcagg cattgagaat ttcgtcgaga tgcaactctt cagaaatctg
     2941 tttaacccat ttttctacac gttcagcggt cagttccggc tgacggtctt cgtcgatagc
     3001 cagaccgaca aagtggtcgt catctgccag accttttgat gcttcgaaat gatagcccgc
     3061 agttggccag tgaccaacga tggttgcacc gcgcggttca atgatgtcgc ggatggtgcc
     3121 caatgcgtcg cagaaatatt cggcgtaatc ttcctggtca ccacaaccaa acagcgcaac
     3181 cagtttgcca ttgaaatcaa tctcttcgag agtcgggaag aagtcatccc agtcacactg
     3241 cgcttcgccg taataccagg ttgggatgcc cagcagcaga atgtcataag cttccagatc
     3301 ttctttgctg ctttttgcaa tgtcatggac atcggcaacg tctttaccaa gctgtttttg
     3361 aatcattttt gcgatatttt cggtattacc ggtgtcgctg ccgaaaaaga tgccagtgat
     3421 agccatgagt gaaataacct cttgaaactt attgaaatgg gggtggaaaa ttgcccacgg
     3481 ataaaggcaa tcatagcaga acaggcagtc ttgcggaatc agcaaacgag caggactgca
     3541 cactgtgcta catgaaagtg gaaatttaaa cgatgccctg actacgcagc gccgccagtt
     3601 gctgcattaa catctcttcg atcagttcgc tacggctcat attgcgcgat tccgccagtt
     3661 cgttcagcgc ctcgacagct tccgcgttca gcttcagttc gacacgctta aggccacgta
     3721 ctttgtcgcg ttttagttgg ttgcgtttat taatacgcag ctgttcatcg cgcgaaagcg
     3781 gattagtttt cggtcgtccc ggtcgacgct cgtgcgcgaa cagatctaat gtcgtacggt
     3841 ccgtttgttc tttggccatg atcttggtga cttcggggga aacaatcagc caggcgcctg
     3901 cccggatgga tagcgcgcca taatacaaca gcgagaggag tctcgccaac gcccacgcgc
     3961 ggaaagcgac gcggacgctg ggtttttaat cagttgcgtt aatcattgag atagcgacgg
     4021 atagcgcgta ataccgcatc tggtttttca gcatggaccc agtgacccgc gcctgcaatc
     4081 acatgcgccc gtgcctgtgg aaattgtgcc agtaaatcat cacggtactg ctcgctaaca
     4141 tacggagaat tgccgccagg gataaacagg gcagggtgat cccatgccgg gattttctcc
     4201 caacccacaa tatgcggata ctgatcccac aataccggca cgttaaagcg ccactccccg
     4261 tcaacaaaag atttcaacag aaactgaatc accccttctt cattaagatg ctggcgcatt
     4321 attgctgctg cttgctggcg agtttgtgcg tccgattcac tgaccgcgtt gatagccgag
     4381 aaaatctcat catgacggcg tacgtgatag tcgaccggtg cgatatcgat cgccaccagt
     4441 ttatcgatgc gatcgggggc cagtgcggta agtgccatta ccgctttacc gcccatggag
     4501 tgaccgataa atgttgcttt gtcgatctgc agtgcatcca gggtatcaac aagatcctgc
     4561 gccatcgtcg ggtaattcat taccggatct ctcggtgaaa gaccgtggtt acgcatatca
     4621 acctggatga tattgtgatc gtttaccaga tcgcgagcca gtacgccaag gttgtcgagg
     4681 ctgccaaaca gaccatggac aagaacgatg ggagaattat tgtgctggtt ttgtgcagtt
     4741 tgcgcgcgga tattcaattt catggcaaag ttcttttttt cgcgttgtcg ggttagggta
     4801 ttatgttgac cattgtgcca cagggctgca acaaataagg tttattccga gtttttctgc
     4861 aagccaggct tgacgctatc cgctgccggg atttattcat atactcctgg cgacttgtat
     4921 tcagctaaga cactgcactg gattaagatg aaaacgattg aagttgatga tgaactctac
     4981 agctatattg ccagccacac taagcatatc ggcgagagcg catccgacat tttacggcgt
     5041 atgttgaaat tttccgccgc atcacagcct gctgctccgg tgacgaaaga ggttcgcgtt
     5101 gcgtcacctg ctatcgtcga agcgaagccg gtcaaaacga ttaaagacaa ggttcgcgca
     5161 atgcgtgaac ttctgctttc ggatgaatac gcagagcaaa agcgagcggt caatcgcttt
     5221 atgctgctgt tgtctacact atattctctt gacgcccagg cgtttgccga agcaacggaa
     5281 tcgttgcacg gtcgtacacg cgtttacttt gcggcagatg aacaaacgct gctgaaaaat
     5341 ggtaatcaga ccaagccgaa acatgtgcca ggcacgccgt attgggtgat caccaacacc
     5401 aacaccggcc gtaaatgcag catgatcgaa cacatcatgc agtcgatgca attcccggcg
     5461 gaattgattg agaaggtttg cggaactatc taaaacgttg cagacaaagg acaaagcaat
     5521 ggcaatccac aatcgtgcag gccaacctgc acaacagagt gatttgatta acgtcgccca
     5581 actgacggcg caatattatg tactgaaacc agaagcaggg aatgcggagc acgcggtgaa
     5641 attcggtact tccggtcacc gtggcagtgc agcgcgccac agctttaacg agccgcacat
     5701 tctggcgatc gctcaggcaa ttgctgaaga acgtgcgaaa aacggcatca ctggcccttg
     5761 ctatgtgggt aaagatactc acgccctgtc cgaacctgca tttatttccg tactggaagt
     5821 gctggcagcg aacggcgtag atgttattgt gcaggaaaac aatggcttca ccccgacgcc
     5881 tgccgtttcc aatgccatcc tggttcacaa taaaaaaggt ggcccgctgg cagacggtat
     5941 cgtgattaca ccgtcccata acccgccgga agatggtggt atcaagtaca atccgccaaa
     6001 tggtggcccg gcagatacca acgtcactaa agtggtggaa gacagagcca acgcactgat
     6061 ggccgatggc ctgaaaggcg tgaagcgtat ctccctcgac gaagcgatgg catccggtca
     6121 tgtgaaagag caggatctgg tgcagccgtt tgtggaaggg ctggccgata tcgttgatat
     6181 ggcggcgatt cagaaagcgg gcctgacgct tggcgttgat ccgctgggcg gttccggtat
     6241 cgaatactgg aagcgtattg gcgagtatta caacctcaac ctgactatcg ttaacgatca
     6301 ggtcgatcaa accttccgct ttatgcacct tgataaagac ggcgcgatcc gtatggactg
     6361 ctcctccgag tgtgcgatgg cgggcctgct ggcactgcgt gataagttcg atctggcgtt
     6421 tgctaacgac ccggattatg accgtcacgg tatcgtcact ccggcaggtt tgatgaatcc
     6481 gaaccactac ctggcggtgg caatcaacta cctgttccag catcgtccgc agtggggcaa
     6541 agatgttgcc gttggtaaaa cgctggtttc ttctgcgatg atcgaccgtg tggtcaatga
     6601 cttgggtcgt aagctggtag aagtcccggt aggtttcaaa tggtttgttg atggtctgtt
     6661 cgacggcagc ttcggctttg gcggcgaaga gagcgcaggg gcttccttcc tgcgtttcga
     6721 cggcacgccg tggtccaccg acaaagacgg catcatcatg tgtctgctgg cggcggaaat
     6781 caccgctgtc accggtaaga acccgcagga acactacaac gaactggcaa aacgctttgg
     6841 tgcgccgagc tacaaccgtt tgcaggcagc tgcgacttcc gcacaaaaag cggcgctgtc
     6901 taagctgtct ccggaaatgg tgagcgccag caccctggca ggtgacccga tcaccgcgcg
     6961 cctgactgct gctccgggca acggtgcttc tattggcggt ctgaaagtga tgactgacaa
     7021 cggctggttc gccgcgcgtc cgtcaggcac ggaagacgca tataagatct actgcgaaag
     7081 cttcctcggt gaagaacatc gcaagcagat tgagaaagaa gcggttgaga ttgttagcga
     7141 agttctgaaa aacgcgtaaa cacattaaat aagaaaaagg gcggtcggaa gatcgccctt
     7201 ttttacgtat gacaaacaca gaattgcctg atgcgctacg cttatcaggc ctacgaggat
     7261 ggtgcaatat attgaattta cgcgattttg taggccggat aaggcgttca tgccggatcc
     7321 agcatttaac cgcctaaagc ataaaccgat aaccaatacc ggtttcagta atgaaatggc
     7381 gggggcgagc gggatcctgt tccagttttt gtcgcagatg ccccatataa atccgcaaat
     7441 aatgactgtg ttcgaccgcg tttggtcccc atacctggtt aaggagctgg cgctgggtga
     7501 gcacttttcc agcattgttg agcagcaccg ccagcaggcg gaattcaatc ggcgtgagat
     7561 gcacctcttc ctcaccccgg tgaatcacgc gggcggctaa atcgacggta acatcggaaa
     7621 attttaccag cggatcgggc gcggcggtgg cggagtggcg gcgtaatgcg acgcgcagac
     7681 gggcctgcaa ttcgccaatg ccaaacggct tactcagata atcatctgct ccggcatcca
     7741 gcgcggcgat tttgtcgctc tcttcgctac gtgcggaaag cacaatcacc ggcaccgcgc
     7801 tccactggcg caggtcgcgg ataaactcaa tcccatcacc atcgggcagg ccgagatcga
     7861 gaataatcaa atctggctta cgggtagccg cttccagcaa gccgcgttgc agcgtttcgg
     7921 cctcatagac gcgcatcccg tcgccctcca gcgccgtgcg cagaaagcga cgaatagcct
     7981 gttcatcttc aacaatcaga acgtttgtca catatcctca tgaaattctt caagttcagg
     8041 ggcagtttgc tggggaagtg taacacgaaa acaggcacca ccttccggtc ggttgaacgc
     8101 ggtaatagtg cccccgtgta catccactat cgcccgacaa attgccagtc caagccctac
     8161 ccccggtact gccgactctt tattcccgcg agcaaactta tcaaatatcg tctgctcctg
     8221 gcctggcgga agaccggggc cgttatccca gacatccagt tgtagatttt cgccctcaac
     8281 gtgggcatcg ataccaattt cggcctgcgc acccgcatat ttcaccgcgt tctccagcag
     8341 attaatcagc acccgttcaa agagtggccc gtcaacgtgg attaaggtca gcggttctgg
     8401 cagagaaaga ttgatgggcg acgataaacc cggttccagc atttgcagcg cgctgccgac
     8461 tacttcttcc agcgttaacc actctttctt caaattaaag ccgccggact gaattcgcgc
     8521 catatccagt agattattca ccagtcgggt agtgttcagc acatgctgac gaatctcgct
     8581 ggcctggcgg gcgtggggtg atccttcgct tgccagatcg agcgttaaga tttctgcctg
     8641 accaaacagc accgtaagcg gagtgcgtaa atcatgcgaa agcgccgcca gcagggcgtt
     8701 gcggatctgt tcacgttcgc ttgccatccg cgcctgttct tcgctggcgg tcagcgtcag
     8761 ccgctcaagg gcattggcga ctaacagcgt aaacgtctcc agcaggcgct gctgttccgg
     8821 gatcatcaac tgacgcagat tccccggttc caccaccaac aacccgtagg ttttctcacc
     8881 gctttttaac ggcaaaatct ggtacggcac gccgggtaac gtgtcggtgc ccgcacccgc
     8941 aggcaggcct ttatcaaaac tccactgcgc gatggcatcg tcccacggcg tcattccttg
     9001 cggatgtgtt aacggctgca atttaccgtt gtcatcgggc aacaacacct gactgcgggc
     9061 atgaaacgtg gaggcaataa attgttcgct ggtggcggcg atgtcctgcg gactgcggcc
     9121 caccgccaga gctttcgaca tttcatataa gtgccgtgtg cgttgctcgc ggtaacgggc
     9181 tacccgtgcc tgataacgca cacccgcagt aaggttcccg atcaccagcc cgacggttaa
     9241 catcaccgcg aaggtcagca gatattgcac atcagagacg gcgagcgtgc cgcgtggggc
     9301 gataaaaaag agatcgaaac tcaccacatt aatgacggta gcaaccaccg aaggccagcg
     9361 tccataaaat agcgccacca ccaccacgcc aagtagatac agcatcacca ggttggcggc
     9421 atcaaacgcc atcagccact gcatggcaat taaggtgata acggcgcata acgcggcggc
     9481 aaccacgcat ccctgaatct gtacccgcca cttgtcttta aaagagcggc tatccggcgc
     9541 gttgttaatc gtgcgggcgg gtggttcatc aagcgcgacc agcacctgat cgagatcggg
     9601 ggcgatgcgc gccagtcggt cagcaaacgt ttcccgacgc caccagcggc gcgaggctgg
     9661 gcgaccgaga ataatcttgc cgagattatg ttcacgggca taacgcacta ccgctttctc
     9721 ttccgctgga tcagaaagtg ttgccgtctc cgcgcccagc tcctgcgcca gacgtaaggc
     9781 gctgagaatt gcccgacgtt ttttctccgg taagcgatgc agggcagggg tttcaacata
     9841 caccgcgtgc cagacgctcc ccagccgtga cgccagccgc gccgccgcgc ggaccagttt
     9901 ttcgctgccg gtgttatggc cgatgcataa aaggatcgcg tcgcgcgtgt gccagacttt
     9961 ctcttcgcca ggatgccccc gccaggcgcg catttgctca tcaacgcgat cggcagtacg
    10021 gcgcagtgcc agttcgcgca gggcgatcag attgcctttg cggaaaaaat gttcaatggc
    10081 gcgctccgcc tgcccggcaa tatagacttt gccttctttt agccgctggc gcagatcgtc
    10141 cgggggcaag tccaccagca ccacgtcgtc ggcggcatcg aaaaaaggat cgggcacggt
    10201 ttcccgtacc tgaattccgg tgacgccgct gaccacatca ttcagacttt ccagatgctg
    10261 aacgttgacg gttgtgaaaa catcaatgcc agcttccagc agttcttcga tatcctgcca
    10321 gcgtttggga tgacgggaac ctggcgcatt actgtgcgcc agttcgtcca ttaagatcag
    10381 cgccggacgg cgggcgaggg cggcatcgag atcaaactcg ctgatatgcc gcccacggta
    10441 cgcctggcgt ttcagcggca gaacagccag cccttccagc attgcggcgg tatctttccg
    10501 cccgtgggtt tctaccacgc caaccacaat atccagccct tgcgcccgca gtcgctgggc
    10561 ttctgccagc atcgcccagg tcttcccgac gcctgcgcag gcaccgaaaa aaactttcag
    10621 cttcccccga tgcggcgcgg cagtttgttc cagcagacga tcggggtcgg gacgtaaggg
    10681 ttcgttattc atcaagttta tccagcgcca gattgagttc gacaatgttg acgaccggct
    10741 ggccgatata tttcaccagc ggttgttggc tgtattttgc gatcagttgc gtgagctgtt
    10801 caacgctgag attacgcgct ttcgctacgc gtgggatctg ccaggccgcc gcttgcgggg
    10861 tgatgttatt gtccagcccg cttgccgacg ccgtcaccag ttcaaccgga acgctcgtgc
    10921 tggcattcgg gttagcggcc cgtaatgcag caacgcgtgc ggctattagt ttatccagct
    10981 cagggttact gaccgccaga ttgctcccgc cagaagcctg tggattataa ggcatttctg
    11041 ccgttgccga cgggcgacca tgaaaatagc cgttgccggt aaaattctgc ccgattaatg
    11101 ccgaaccccg caccgtatca ccttcacgga tcaacgaacc gttggcctgc cagggaaacc
    11161 accattgccc cagcgcggtg gtcagcagcg ggtaaacacc gccagtaatc aataacagaa
    11221 agataaatgt cgataatgcc ggacgtaatc cacgcatggt aaacctcaca ccagaccgca
    11281 aacggtcagt agtaaatcaa tgactttgat accaataaac ggcaccagca gcccacccag
    11341 accgtaaatc cataagttac ggcgcaacat ggcagaagcg gtaagcggtt tataactcac
    11401 gccttttaac gccaggggaa tcaaaaagac gataatcaag gcgttgaaaa tgaccgcact
    11461 gagaatggcg gagtcgggcg aatgcaggcg catgatgttc agcgcattta actgtggata
    11521 cgttaccgcg aatgccgccg gaataatggc gaagtatttc gccacatcgt tggcaatgct
    11581 gaaggtggtc agcgagccac gggtcatcag catctgtttg ccaatatgta ccacctcgat
    11641 caacttggtc gggttagagt cgagatccac catattgccc gcttctttcg ccgcctgagt
    11701 accggagttc atcgccaccg cgacatctgc ctgcgccagc gccggagcat cgttggtgcc
    11761 gtcgccggtc attgctacca aacggccttc cgcctgatac tgacggatca atgccagctt
    11821 ggcctccggt gtcgcttcgg cgagaaaatc atcgacgccc gcttccgcgg caatggcggc
    11881 ggcagtcaga cggttatcgc cggtaatcat caccgtttta atacccattt tgcgcagttg
    11941 ggcgaagcgt tctttaatgc cgcctttgac gatatctttc aacgcaataa cgcccagcac
    12001 acgagaacct tctactacta ccagcggtgt ggccccctga cgcgcaacct gatcgacttt
    12061 ttgatcaaca tcggcaggga agtgaccacc gttagcctca acatggcggc gaatggcatc
    12121 gacagaacct ttacggatca tgcggttgtc gatattgatc ccgctcatcc ggctttgcgc
    12181 agtaaacggt acaaaggtgg catggagcga ctgcacatcg cgctcgcgca ggttaaaacg
    12241 ctgcttggcg aggatcacaa tactgcggcc ttccggcgtt tcatcagcca gcgaagccag
    12301 ttgtgcggcg tcagccagcg ttttttcatc cacgccctgc gcagggataa actccgatgc
    12361 ctgacggtta ccgagtgtga tggtgccggt tttatccagc agcagaacgt caacgtcacc
    12421 tgccgcttca actgcacgtc cgctggtggc aatcacattc gcgcccagca tccgactcat
    12481 cccggcgacg ccgatcgctg acaacaggcc gccaatagtg gttgggatca gacagaccag
    12541 cagcgccacc agtaccgtta cgctgactgc attaccgccc cacgcggaaa acggccacag
    12601 cgtggcggtt gccagtaaaa agacgatagt cagggcaatc agcaggatgg tcagggcaat
    12661 ctcgttcggc gttttgcgtc gctgtgcgcc ttccaccatc gcgatcatcc gatccagaaa
    12721 tgtctcgccg gggttaacgc tacactcaat caccagccag tcagaaagaa tacacgtgcc
    12781 gccggtgacg gaggcaaaat cgccgccgga ttcacggatc accggtgccg attccccggt
    12841 gatggcgctt tcatcgaccg atgcgccgcc ttcaataact tcaccatcgc aggggataat
    12901 atcgcctgct tctaccagta cgatatcgcc tttacgcagt tgatcggcgg gaactttgtc
    12961 cgccgcagcg ccatatttcg gttcgcgcag cttgcgggca aaggccgttt ttttcacccc
    13021 tttcaggctg ttggcctgcg ctttactgcg gccttctgcc agcgcctcgg cgaaattagc
    13081 gaacagtacg gtgacccaca gccagccgct aatggccacg ctaaacagcg cattgccagg
    13141 catcgcgccg cttgccatcg cgatgctaat acaggtggtc agcagactgc cgatccagac
    13201 gataaacatc accggattgc gccattgcgc ctgcgggttt aattttttca ccgcttcttt
    13261 cagcgcctga acgacaagtg ttggttcgaa tagcgccagt tgtttacgac tcatattcag
    13321 tgctcactca atatcatcag gagagatatt ccgccaccgg accaagcgcc agggcaggga
    13381 taaaggtcag tgcgccaacc agcaacacgg tgccgattaa caggccaaca aacagcgggc
    13441 cgtgcgttgg cagcgtgccg gagctggcgg gttggctctt tttactcacc agcgaaccgg
    13501 caattgccat caccgggata atcaccccga agcgaccgac aaacatgcag aacgccagta
    13561 aacagttcca gaacggagag ttggcgctta atccggcaaa ggcgctgccg ttgttgttag
    13621 cggcggatga cacggcgtac agcacttcgc taaaaccatg cgggccaggg ttgagcatgg
    13681 cgctacgtcc ggcgtcggtc atcattgcca gcgccgcgcc catcagcacc agcgtcgggg
    13741 tgaccagaat cgccagtgca gtcagtttca tctcacgtac gtcgattttt ttacccagat
    13801 attccggtgt acgaccaatc atcagcccgg caataaacac cgccagcagg acaaacagca
    13861 tcatgccgta aagaccagaa ccgacaccgc cgaataccac ttcaccaatt tgcatcagcc
    13921 acatcggcac catgccaccg agagcggtaa acgaatcatg catcgcaatc accgcgccac
    13981 aggacgccgc cgtagtcacg accgcaaaca ggctactgac cagcacgccg aaacggctct
    14041 ctttaccttc catattgatg ctgctgtctg cgccgagcgc cagcagatga ggattaccct
    14101 gaacttctgc ccacatcacc acgcctacgc agatgacaaa aatcactgac atcgcccaca
    14161 gcaacatgcg cccctggcgg cgatcgcccg tcacttcacc aaaggcaaag cacagcgctg
    14221 ttgggatcaa gaagatcgcc agcatctgca cgaagttggt cagtgcggtt gggttttcaa
    14281 acggatgcga cgagttggca ttaaagaagc caccgccgtt agtgccgagc atcttgatcg
    14341 cttcctgaga agccactggc cccattggta acagctgctg cgctccttca acggtcgtca
    14401 cagcctgata aggctgaaaa ttttgcagtg caccttgttg aataaaaaac agcgcgatca
    14461 acaacgccac aggggccaac acccataacg tgatgcgtag cagatcgacc caggcattcc
    14521 cgagtgtgct catgctctgg cgggtaaatg cacggatgag ggaaaaaatc accgcaatcc
    14581 cgctggcggc agaaagaaag ttttgcaccg ttaagcccgc catctggctg aaatagctca
    14641 acgtggtttc accgctataa gattgccagt tggtattagt gacaaagctg acggcagtat
    14701 tcagcgccag atcccacgac agccccggca actgctgtgg attaagcggc agatagtgct
    14761 gaccgagcag catgaaaaaa agcaccgcca gccccagcat gttcagacag agaatggctg
    14821 agagatactg cttccagttc atctcacggt cagagacgcc aagcgcgcta aagagtacgc
    14881 gttcaacgcc cgctgtaccg ggaagaggaa tatcattaat caaccgtgcc agcccgctgc
    14941 cgaaaggacg cgccagcacc attaatacca gtaaaaacgt ggcaatcagt aagaaccctt
    15001 gcgcagccat cagaacgcct ccgcattgat cagggcataa accagataac ccagtaataa
    15061 aaacaccagc aatacgccgg ttatcacgcc tgcactcaca gtgcacctcc agtggcctaa
    15121 aagtgatacc ggaagggtag aattctggct gcaaagattt cgcaaaaatc tgcggacggg
    15181 gtgtaaaaaa agtataaaaa tggcaaaagc catgatttaa ctaatgttta gtat
//
LOCUS       DAZGBC010000116        15230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGBC010000116 DAZGBC010000000
VERSION     DAZGBC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.117"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHK1J_003494"
     CDS             complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHK1J_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO7912721.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            573..2771
                     /gene="priA"
                     /locus_tag="ACHK1J_003495"
     CDS             573..2771
                     /gene="priA"
                     /locus_tag="ACHK1J_003495"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO7912722.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2927..3952
                     /gene="cytR"
                     /locus_tag="ACHK1J_003496"
     CDS             2927..3952
                     /gene="cytR"
                     /locus_tag="ACHK1J_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO7912723.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHK1J_003497"
     CDS             4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHK1J_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO7912724.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5096..5626
                     /gene="hslV"
                     /locus_tag="ACHK1J_003498"
     CDS             5096..5626
                     /gene="hslV"
                     /locus_tag="ACHK1J_003498"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO7912725.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5636..6967
                     /gene="hslU"
                     /locus_tag="ACHK1J_003499"
     CDS             5636..6967
                     /gene="hslU"
                     /locus_tag="ACHK1J_003499"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO7912726.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7034..7960
                     /gene="menA"
                     /locus_tag="ACHK1J_003500"
     CDS             7034..7960
                     /gene="menA"
                     /locus_tag="ACHK1J_003500"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO7912727.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8053..8538
                     /gene="rraA"
                     /locus_tag="ACHK1J_003501"
     CDS             8053..8538
                     /gene="rraA"
                     /locus_tag="ACHK1J_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO7912728.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            8598..9272
                     /locus_tag="ACHK1J_003502"
     CDS             8598..9272
                     /locus_tag="ACHK1J_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912729.1"
                     /translation="MQNIILLFIYLLLIAVNHFRYKFLLSGNAGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            9395..10021
                     /locus_tag="ACHK1J_003503"
     CDS             9395..10021
                     /locus_tag="ACHK1J_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912730.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(10060..10305)
                     /gene="zapB"
                     /locus_tag="ACHK1J_003504"
     CDS             complement(10060..10305)
                     /gene="zapB"
                     /locus_tag="ACHK1J_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO7912731.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10731..11576
                     /gene="glpF"
                     /locus_tag="ACHK1J_003505"
     CDS             10731..11576
                     /gene="glpF"
                     /locus_tag="ACHK1J_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO7912732.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            11599..13107
                     /gene="glpK"
                     /locus_tag="ACHK1J_003506"
     CDS             11599..13107
                     /gene="glpK"
                     /locus_tag="ACHK1J_003506"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO7912733.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            13337..14347
                     /gene="glpX"
                     /locus_tag="ACHK1J_003507"
     CDS             13337..14347
                     /gene="glpX"
                     /locus_tag="ACHK1J_003507"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7912734.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            14444..15190
                     /gene="fpr"
                     /locus_tag="ACHK1J_003508"
     CDS             14444..15190
                     /gene="fpr"
                     /locus_tag="ACHK1J_003508"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO7912735.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKESSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
BASE COUNT         3722 a         3822 c         4064 g         3622 t
ORIGIN      
        1 taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca acaggaaatg
       61 ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta caggcacaaa
      121 aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc gggatgttga
      181 aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt ttgccagtga
      241 agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg gtggagcgga
      301 ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc gggtgaatat
      361 cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc taacgccaga
      421 caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc
      481 atacagaaat taaccagcgt atgcaaactg atccgcactc ttctacggca atgtgtatac
      541 taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt gccttgcccg
      601 ttccgcttcc tcgtaccttt gactatctgc tgccagaagg catgacggtt aaagctgggt
      661 gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cgggattgtg gtatcagtta
      721 gcgatgccag cgaactgccg ctcaatgagc taaaagcggt agtcgaagtg ctggatagtg
      781 agccggtgtt tactcactcc gtctggcgat tgctgctatg ggcggcagat tattatcatc
      841 atccgattgg cgatgtgctg tttcatgcct tgccgatttt actacgccag gggcggcctg
      901 cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccatgcg gtggatctga
      961 acagcctgaa acgttcgcca aagcaacaac aggcactggc ggcattgcgg caaggcaaaa
     1021 tctggcgcga ccaggttgcc acgcttgaat ttaatgatgc cgcgttacag gcgctgcgca
     1081 aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg cgaacgaact
     1141 atgccgtttc tggtgagcgg ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa
     1201 ttcatagtgc ggcggatacc ttctctgcct ggctgctggc gggtgttaca ggttccggta
     1261 aaacagaggt ttatttgagc gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg
     1321 tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta
     1381 atgcccccgt agaagttctg cattccggcc tgaacgacag cgagcgtctt tcggcgtggt
     1441 tgaaagcgaa aaatggtgag gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc
     1501 cgtttaaaaa tctcggtgtg attgtcattg atgaagagca cgacagttcc tacaagcagc
     1561 aggaaggctg gcgctatcat gcccgcgacc tggcggttta tcgtgcgcac agcgagcaaa
     1621 tcccgattat tcttggctcc gcaacacccg cactggaaac gttatgcaac gtccagcaga
     1681 aaaaataccg cctgctgcgc ctgacgcgtc gggcggggaa tgcgcgtccg gcaattcaac
     1741 acgtgctgga tttaaaaggt cagaaggtgc aggcggggct ggctccggcg ttaatcaccc
     1801 gtatgcgcca gcatttacag gccgacaacc aggttatcct ctttcttaac cgccgtggct
     1861 ttgcgcctgc cttgctgtgc cacgactgcg gctggattgc cgaatgtcca cgttgcgatc
     1921 actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt gacagccaac
     1981 gcccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtaccc gtggggctgg
     2041 gcaccgaaca gcttgaacag acgctcgcgc cgttattccc tggtgtgccc atttctcgta
     2101 tcgaccgcga taccaccagc cgcaaaggag cgctggaaca acaactggcg gaagtgcatc
     2161 gcggcggcgc gaggatcttg attggcacgc aaatgctggc gaaaggtcac cactttccgg
     2221 atgtgacgct ggtagcgtta ctggatgtgg acggcgcgct gttttctgcc gattttcgct
     2281 cggcagagcg ttttgcccag ctatacaccc aggtagcagg ccgtgccggg cgtgcgggta
     2341 aacagggcga agtggtgctg caaacgcatc atccggaaca cccgctattg caaacgttgc
     2401 tctataaagg ctacgacgct tttgccgaac aggcactggc tgagcgacgc atgatgcagc
     2461 tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc
     2521 cgctgttcct gcaacaactg cgtaatctga tcctctccag cccgctggca gacgacaaat
     2581 tgtgggttct cggtccggtt ccggcactgg cacctaaacg tggcggtcgc tggcgctggc
     2641 agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattaac ggtacgctgg
     2701 cactcatcaa tacaataccg gattcccgta aggtgaaatg ggtgctggat gttgatccga
     2761 ttgagggtta aaccgctcac gatgcgaggc ggatcgaaaa attcaatatt catcacactt
     2821 ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa
     2881 gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa
     2941 gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac
     3001 cgtctcccga gcattaatga atcccgataa agtctctcag gccacccgta atcgggttga
     3061 aaaagcggcc cgggaagtgg gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa
     3121 tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct ttagcgaaat
     3181 tattcgcggt atagaagtta cggcggcaaa tcacggatat ctggtgctga ttggcgactg
     3241 tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca agcaaattga
     3301 tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg aacagcgtaa
     3361 tctgccgccg atggtgatgg cgaacgaatt tgcaccggaa ctggagctgc ctaccgttca
     3421 tatcgacaat ctgaccgccg catttgatgc agtaaattat ttatatgagc aagggcataa
     3481 acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg
     3541 ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca tcgcccgtgg
     3601 tgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc
     3661 gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa
     3721 acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata acatcgacct
     3781 gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg
     3841 ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca gtggctctcg
     3901 tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt aaagtaaacc
     3961 tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg ggctgacgaa
     4021 cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc agccaaccgg
     4081 caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg cctgcggttt
     4141 ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt ggtctgtact
     4201 tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa gtgaccggaa
     4261 acggactacc accaaaacca gaagaacgct ggcgctacat caaagagctg gaaagtcgcc
     4321 agccgggtgt gcgtgcgccc acagaacctt ctgctggtgg tgaagtgaaa acgccggagc
     4381 aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg cgccagcagc
     4441 caacgcagct ggttgaggtt ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc
     4501 tacagcgcca acgtcaggcg cagcaattag cggaacagca acgcctggct cagcagtcgc
     4561 gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg
     4621 cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa
     4681 cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt actcgtgccg
     4741 ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg
     4801 gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg ttcgaaggct
     4861 ttgactcaaa aattaccacc aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag
     4921 gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat acaaactgca
     4981 ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa ttgcattatg
     5041 ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc agctcgtgac
     5101 aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg gtcaggccac
     5161 gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa
     5221 agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg aactgtttga
     5281 acgtaaactg gaaatgcatc agggccatct ggttaaagcc gccgttgagc tggcaaaaga
     5341 ctggcgtacc gatcgcatgt tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac
     5401 cgcttcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg atcttattgc
     5461 tatcggctcc ggcggccctt acgcccaggc tgcggcgcgc gcgctgttag aaaacactga
     5521 acttagcgcc cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta
     5581 taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc
     5641 tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg gccaggacaa
     5701 cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga
     5761 agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg
     5821 taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca tcaaagttga
     5881 agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta ttattcgcga
     5941 tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa accgttatcg
     6001 cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg ctaaaaacaa
     6061 ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa
     6121 actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg cagcaccgat
     6181 gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc tgcagtccat
     6241 gttccagaac ctgggcggtc agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat
     6301 gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc tgaagcaaga
     6361 cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg acaaaatctg
     6421 taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc gagacctgct
     6481 gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa ctgaccacat
     6541 tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga tcccggaact
     6601 gcaaggtcga ctgccaatcc gcgttgaact gcaggcactg accaccagcg acttcgagcg
     6661 tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga tggcgactga
     6721 aggcgtaaat atcgagttta ccgactccgg tattaaacgc atcgcggaag cggcatggca
     6781 ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc tggagcgttt
     6841 aatggaagag atttcctacg acgccagcga tttaagcggt caaaatatca ctattgacgc
     6901 agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga gccgttttat
     6961 cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg ccccattttt
     7021 attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc tggaaagttt
     7081 acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg
     7141 gtggcaaggt cattttgatc cgctggtcgc cctgctggca ctgattaccg ccgggctatt
     7201 acagatcctt tctaacctcg ccaatgatta cggcgatgca gtaaaaggca gcgataaacc
     7261 tgaccgcatt gggccgctac gcggcatgca aaaaggggtc attacccagc aagagatgaa
     7321 acgggcgctc attattaccg tcgtgctcat ctgtctctct gggctggcac tggttgcagt
     7381 ggcatgccat acgctggctg attttgtcgg tttcctgatt cttggcgggt tgtcgatcat
     7441 tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc tgggtgatat
     7501 ttccgtactg gttttctttg gctggttaag cgtcatgggg agctggtatt tacaggctca
     7561 tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg caacggcagt
     7621 actgaacatt aataacctgc gtgatatcaa tagcgaccgc gaaaacggca aaaacacgct
     7681 ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc
     7741 tctggtatgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg gctggctgtt
     7801 cctgctggcg gcaccgttgc tggtgaagca agcccgttat gtgatgcggg aaatggaccc
     7861 ggtggcgatg cgaccgatgc tggaacgcac tgtcaaggga gcgttactga ctaacctgct
     7921 gtttgttgta gggatattcc taagccagtg ggcagcataa ctgacaaata tcaattaaca
     7981 attgatgatt ttgccaacag accacatagc gcgatatact gaaaattctc gcagcaactg
     8041 aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca agaagatgtt
     8101 aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg cggacaaata
     8161 atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga acagaatggc
     8221 cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct
     8281 gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg
     8341 cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc ggcaattccg
     8401 gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtt
     8461 accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct ttcagaagat
     8521 ccgctggata ttgaataagt atctgccatg cgttaaaagg ggaattaaat tccccttttt
     8581 tatacatgga cgtattcatg cagaatatta ttcttttatt tatatattta ttacttatag
     8641 ccgttaacca tttccgctac aaatttcttt tatctggcaa cgcgggtgaa atgattttat
     8701 attttgctaa cgtatttttt aacccacttg ccttatcatt tattatcgcc acaatcatct
     8761 gtataacgat aaaaaaaaga agcagccgca gctttattcg cggcacttgt tggttaatgg
     8821 gattatttct tatctatagc agctatacgg tgtgggaacg acacagcata tgggattata
     8881 ctttcccgga gccaggtatc accgtcacgg ttccgagcaa acagtgggtt gccaatatag
     8941 taaaacaagg accaaccctg gtgacccggg atgcccatgc tattttagct attgttgcct
     9001 tctcacaaaa tgaactcggt gtgcattcaa tcgaggaact taccgcgact caaaacggaa
     9061 ctgcagaaat gcatgtttgt gatgttcagg gatttgcctg cgcttatcag gaaggagtgc
     9121 aaacaatgag tgatggcaaa gtaagacatg ctattttcat gactctactg gataacacta
     9181 atttgataca actggtcgca gctatcgacc cagattattt ggacgaatat caagaagaag
     9241 taatgaaaat gatgctttct gctcgtcaat gatctgatgt gcatgttaat aataatggat
     9301 tgatatgaga tacgttatct tattaatact atggataata actgtttttc ttagtcatct
     9361 tcagcacagt aacttgctta ctggagatcc tgggatggtt ggcgagtata tcggttcagt
     9421 acttcttggg ccgttattac ttcccgtgct tgtgtcaggt attctgtgca cattcactaa
     9481 aaaaacgcgt aacttcgcca gttttactcg cgggtgctgt tgggtattgg gagtcctgct
     9541 tttatccaac gtcggcaata cgtttcgtct gttcaccccg tggcactata cgtttgagaa
     9601 ggcagctatt tccgtgactg tcccaaaccg acactggaat acggtttcta ttagtactga
     9661 taaaaccatt gatataagaa gtgaagataa tagcgtcttt atttctgctt tccgcctgcc
     9721 cgcaggacgt agcgccgatg actcgctgga agagttaaag aagatgcagc gagacaatct
     9781 caaggatcaa tacaatgaag aaaccttcca gtttcatgat tgtaatgcca aacatttcac
     9841 ctgtaagtat caggatgtgt tgataaattt tgatggtcag cagaaaagaa ccatcagtgt
     9901 ttatcttgaa gacaccccca gggctgtggg gatcattgca ttaatggagc cagatacggc
     9961 agataaatat cgccaacagg cgatggagat tatgttgtct gctaaaaata cagtgaagta
    10021 aaaaataagg cgccttgcgg cgcctctgcg tggaagagat taaacctctt ccatgcgacc
    10081 cagcagggcc tgcagacgtt cctgccagcc gttctgctgt tctttcagat ggttgttctc
    10141 acgctccagc tcttcgcgct gatgctgggc attttgaact tcctgcgaca gtgagttgtt
    10201 tttttctttc agctcttcga tttccatctg caacagagtg atggtatcaa tcgcctgctg
    10261 tacttttgct tccagtttct caaacacttc taatgacatt gtcatacctc tcctgaattg
    10321 caaggcgttg atggataaaa aatcctcgtc ccgattaccg gtgacgcctt aataaatacg
    10381 agcgcacttt agttagctcc gattgtatga agccgcgcca tcgctgtcca gcggcacgcc
    10441 ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat ccacaccaat
    10501 cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca tgtacaatca
    10561 gatggaataa atggcgcgat aacgctcatt ttatgacgaa gcacacacat tttaagttcg
    10621 atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag catcgtggag
    10681 gtccgtgact ttcacgcata caacaaacat taactcttca ggatccgatt atgagtcaaa
    10741 catcaacctt gaaaggccag tgcattgctg aattcctcgg taccgggttg ttgatttttt
    10801 tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc gtcttttggt cagtgggaaa
    10861 tcagtgtcat ttggggactg ggggtggcaa tggccatcta cctgaccgca ggggtttccg
    10921 gcgcgcatct taatcccgct gttaccattg cattgtggct gtttgcctgt ttcgacaagc
    10981 gcaaagttat tccttttatc gtttcacaag ttgccggcgc tttctgtgct gcggctttag
    11041 tttacgggct ttactacaat ttatttttcg acttcgagca gactcatcac attgttcgcg
    11101 gcagcgttga aagtgttgat ctggctggca ctttctctac ttaccctaat cctcatatca
    11161 attttgtgca ggctttcgca gttgagatgg tgattaccgc tattctgatg gggctgatcc
    11221 tggcgttaac ggacgatggc aacggtgtac cacgcggccc tttggctccc ttgctgattg
    11281 gtctactgat tgcggtcatt ggcgcatcta tgggcccatt gacgggtttt gccatgaacc
    11341 cagcgcgtga cttcggtccg aaagtctttg cctggctggc gggctggggc aatgtcgcct
    11401 ttaccggcgg cagagacatt ccttacttcc tggtgccgct ttttggccct atcgttggcg
    11461 cgattgtagg tgcatttgcc taccgcaaac tgattggtcg ccatttgcct tgcgatatct
    11521 gtgttgtgga agaaaaggag accacaactc cttcagaaca aaaagcttcg ctgtaatatg
    11581 actacgggac aattaaacat gactgaaaaa aaatatatcg ttgcgctcga ccagggcacc
    11641 accagctccc gcgcggtcgt aatggatcac gatgccaata tcattagcgt gtcgcagcgc
    11701 gaatttgagc aaatctaccc aaaaccaggc tgggtagaac acgacccaat ggaaatctgg
    11761 gccacccaaa gctccacgct ggtagaagtg ctggcgaaag ccgatatcag ttccgatcaa
    11821 attgcagcta tcggtattac gaaccagcgt gaaaccacta ttgtctggga aaaagaaacc
    11881 ggcaagccta tctataacgc cattgtctgg cagtgccgtc gtaccgcaga aatctgcgag
    11941 catttaaaac gtgacggttt agaagattat atccgcagca ataccggtct ggtgattgac
    12001 ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc atgtggaagg ctctcgcgag
    12061 cgtgcgcgtc gtggtgaatt gctgtttggt acggttgata cgtggcttat ctggaaaatg
    12121 actcagggcc gtgtccatgt gaccgattat accaacgcct ctcgtaccat gttgttcaac
    12181 atccataccc tggactggga cgacaaaatg ctggaagtgc tggatattcc gcgcgagatg
    12241 ctgccagaag tgcgtcgttc ttccgaagtg tacggtcaga ctaacattgg cggcaaaggc
    12301 ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc aggccgcgct gtttggtcag
    12361 ttgtgcgtga aagaagggat ggcgaagaac acctatggca ctggctgctt tatgctgatg
    12421 aacactggcg agaaagcggt gaaatcagaa aacggcctgc tgaccaccat cgcctgcggc
    12481 ccgactggcg aagtgaacta tgcgttggaa ggtgcagtgt ttatggcagg cgcatccatt
    12541 cagtggctgc gcgatgagat gaagttgatt aacgacgcct acgattccga atatttcgcc
    12601 accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg catttaccgg gctgggtgcg
    12661 ccatactggg acccgtatgc gcgcggggcg attttcggct taacgcgtgg agtgaacgct
    12721 aaccacatta tccgcgcgac gctggagtct attgcttatc agacgcgtga cgtactggaa
    12781 gcaatgcagg ccgactccgg tattcgtctg cacgccctgc gcgtggatgg cggcgcagta
    12841 gcaaacaatt tcctgatgca gttccagtcc gatattctcg gcacccgcgt tgagcgcccg
    12901 gaagttcgcg aagtgactgc attgggtgcg gcctatcttg ctggtctggc ggtcggtttc
    12961 tggcagaacc tcgacgagct gcaagagaaa gcggtgattg agcgcgagtt ccgtccaggc
    13021 atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga aaaaagcggt caaacgcgcg
    13081 atggcgtggg aagaacacga cgagtaatgt caggcacctg aatcgattgc ctgatgctga
    13141 tcgcctgatg cgacgcttac gcgtcttatc aggcctacga gccgatgcca ttttgtgtag
    13201 gcgggataag gcgttcacgc cgcatccggc atcacgcaga ctctgaaacc gtgcgggggc
    13261 aaccccgcac acatcaataa tccctccctt cccctgtgct acacttcgcg ccattcctta
    13321 ctgcttagag tttgctatga gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc
    13381 agcggcgctg gctggctaca aatggttagg acgcggcgat aaaaacaccg cggacggcgc
    13441 ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac attgacggca ccatcgtcat
    13501 tggtgaaggt gaaatcgacg aagcaccgat gctctacatt ggtgaaaaag tcggtactgg
    13561 tcgcggcgac gcggtagata ttgctgttga tccgattgaa ggcacgcgca tgacggcgat
    13621 gggtcaggct aacgcgctgg cggtgctggc agtaggcgat aaaggctgtt tcctcaacgc
    13681 gccagatatg tatatggaga agctgatcgt cggaccggga gccaaaggca ccattgatct
    13741 gaacctgccg ctggcggata acctgcgcaa tgttgcggcg gcgctcggca aaccgttgag
    13801 cgaactgacg gtaacgattc tggctaaacc acgccacgat gccgttatcg ctgaaatgca
    13861 gcaactcggc gtacgcgtat ttgctattcc ggatggcgac gttgcggcct caattctcac
    13921 ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt
    13981 ggtttctgcg gcggtgatcc gcgcattaga tggcgacatg aacggtcgtc tgctggcgcg
    14041 tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg
    14101 ctgcaaagcg atgggcatcg aagccggtaa agtattgcgt ctgggcgata tggcgcgcag
    14161 cgataacgtc atcttctctg ccaccggtat taccaaaggc gatctgctgg aaggcattag
    14221 ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc cgcggcaagt cacgcaccat
    14281 tcgccgcatt cagtccatcc actatctgga tcgcaaagac ccggaaatgc aggtgcacat
    14341 cctctgattg atttgatcga ttgagccttc cagtccttcg ggactggaat ttttttgttc
    14401 ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa aacatggctg attgggtaac
    14461 aggcaaagtc actaaagtgc agaactggac cgacgccctg tttagtctca ccgttcacgc
    14521 tcccgtgctt ccgtttaccg ccgggcaatt taccaagctt ggccttgaaa tcgacggcga
    14581 acgcgtccag cgcgcctact cctatgttaa ctcgcccgat aatcccgatc tggagtttta
    14641 cctggtcacc gtccccgatg gcaaattaag cccacgattg gcggcactga aaccgggcga
    14701 tgaagtgcag gtggttagcg aagcagctgg cttctttgtt ctggatgaag taccagattg
    14761 cgaaacgcta tggatgctgg caaccggtac agcgattggc ccttatttat cgattctaca
    14821 actaggcaaa gatttagatc gcttcaaaaa tctggtcctg gtgcacgccg cacgttatgc
    14881 cgccgactta agctatttgc cactgatgca agaactggaa aaacgctacg aaggaaaact
    14941 gcgcattcag acggtggtca gtcgggaaac ggcagcgggt tcgctcaccg gacggatacc
    15001 ggcgttaatt gaaagtggtg aactggaaag cgcggttggc ctgccgatga ataaagaaag
    15061 cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc gatacgcaac agttgctgaa
    15121 agagacccgg cagatgacga aacatttacg tcgccgaccg ggtcacatga cagcggagca
    15181 ttactggtaa gcggttactt atcgataaac ggcacgatca agagatccgc
//
LOCUS       DAZGBC010000117        15184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGBC010000117 DAZGBC010000000
VERSION     DAZGBC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.118"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..840
                     /gene="hyxR"
                     /locus_tag="ACHK1J_003509"
     CDS             46..840
                     /gene="hyxR"
                     /locus_tag="ACHK1J_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO7912736.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            994..1755
                     /gene="hyxA"
                     /locus_tag="ACHK1J_003510"
     CDS             994..1755
                     /gene="hyxA"
                     /locus_tag="ACHK1J_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO7912737.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTDSVCNITYIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1797..4094
                     /gene="hyxB"
                     /locus_tag="ACHK1J_003511"
     CDS             1797..4094
                     /gene="hyxB"
                     /locus_tag="ACHK1J_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFO7912738.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADNAVSTTPVTLDKDKTT
                     LDQDVVINGDKKITAVTIETSDSDKDLNVTFGGHDITAASTVNQDFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVIDSTGDVVVNGGNFVAKNEKGSATGISLEGAKGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSLLNSFEGDATDNITLAGSNIINGRIETIV
                     TAGNKTGIHTVNLNIKDGSVIGAANNKQTIYASASAQGTGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYTFNSGDKAGSNLDINLSDSSVWKGKVS
                     GAGNASVSLQNESVWNVTGSSTVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASS
                     ENTLDISGKASGDLRVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQG
                     ADGNFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWI
                     QYFGGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSK
                     GDTEGYSFHAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAM
                     VKGGYTWEDGNGLFIQPYAKLSALTLEGVDYQLNGVNVHSDSYNSVLGEAGTRVGYDF
                     AVGNATVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAY
                     ASLDYTKGDDIENPLQGVVGINVTW"
     gene            4276..4944
                     /gene="ykgH"
                     /locus_tag="ACHK1J_003512"
     CDS             4276..4944
                     /gene="ykgH"
                     /locus_tag="ACHK1J_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO7912739.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIINWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLILGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(5187..5882)
                     /gene="ykgG"
                     /locus_tag="ACHK1J_003513"
     CDS             complement(5187..5882)
                     /gene="ykgG"
                     /locus_tag="ACHK1J_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO7912740.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5875..7302)
                     /gene="ykgF"
                     /locus_tag="ACHK1J_003514"
     CDS             complement(5875..7302)
                     /gene="ykgF"
                     /locus_tag="ACHK1J_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO7912741.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLIAMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7313..8032)
                     /gene="ykgE"
                     /locus_tag="ACHK1J_003515"
     CDS             complement(7313..8032)
                     /gene="ykgE"
                     /locus_tag="ACHK1J_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO7912742.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(8559..9413)
                     /gene="rclR"
                     /locus_tag="ACHK1J_003516"
     CDS             complement(8559..9413)
                     /gene="rclR"
                     /locus_tag="ACHK1J_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO7912743.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGMDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQFAP"
     gene            9639..10964
                     /gene="rclA"
                     /locus_tag="ACHK1J_003517"
     CDS             9639..10964
                     /gene="rclA"
                     /locus_tag="ACHK1J_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO7912744.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGVAVNERGGIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLIK"
     gene            11073..11309
                     /gene="rclB"
                     /locus_tag="ACHK1J_003518"
     CDS             11073..11309
                     /gene="rclB"
                     /locus_tag="ACHK1J_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO7912745.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            11321..11914
                     /gene="rclC"
                     /locus_tag="ACHK1J_003519"
     CDS             11321..11914
                     /gene="rclC"
                     /locus_tag="ACHK1J_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO7912746.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(12074..12943)
                     /locus_tag="ACHK1J_003520"
     CDS             complement(12074..12943)
                     /locus_tag="ACHK1J_003520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO7912747.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLFHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            13201..14051
                     /locus_tag="ACHK1J_003521"
                     /pseudo
     CDS             13201..14051
                     /locus_tag="ACHK1J_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(14191..14748)
                     /locus_tag="ACHK1J_003522"
                     /pseudo
     CDS             complement(14191..14748)
                     /locus_tag="ACHK1J_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4160 a         3526 c         3403 g         4095 t
ORIGIN      
        1 gaatcaaaat gtcaggtttt gtagcaatgg cgtggacgag tctgaatgaa gaagcctctg
       61 gttattatca gtgcctgtca gttcaccagg ctggcactgg agtctctcat cccagcggac
      121 aggtatatcg tacgggtgta cagcaacgtc actacagagg ttgaattcgt tttaaagacc
      181 tcctgtggct atctgttggc tgatatccct tcgcttcctt cgggggagtt agttcttctg
      241 gcttgtcttg tccgcttgcg gagtgatgcc gggcaatggc aggtctgcct gaccggtgac
      301 tccgactggt tcgataatgc tcctgctgca agtctgtata acggcttccc acgcatcggc
      361 accatgctga cagcgagtcg ttatcagaat caaattatcc gctggttact acgtcctttg
      421 cccggcgata tgagcgattt ctggctcctg actgcacggg agttgagcgt gcttaagatt
      481 ctgatgcagg gcatgagttt cagtgagatt gcccgttatg aacaacgcag ttcaaagacc
      541 ctgcatgcga ttgccacacg ggcattaatg aaacttggac tgggaactct gagcgatttc
      601 aggctgctct atacgggatg cggtgacagc agagtgaaac ggaatatacg gctacatgca
      661 aaatatgtcg gacaacattc cgctcatcga ctcactcagt atgtgcagaa catggtatcc
      721 ggaattcttt atccagagaa agttgccgtt ggtgaatcaa taaattctga cgccatatcc
      781 ggaaaagtac tttccggaca aagaaaaaat atgtctaaaa aaagaccagg attgttatga
      841 cacagcagcg ttgttactac cccccactgc ggcgcgtacg gactcgctac agtgtagggg
      901 ccaaaatatt tctactgctc tgggctgcag gattgggagg cacgctgtac agcctgtaca
      961 gcgtgctggc gtactgacat ctgtgaggga aggatgcgtt tcgttctttt ctgtccctca
     1021 ggaattgtac ctgcacagtt cgcagctcta tcaaccgatt ctgtctgcaa tattacctac
     1081 atcagaacag aagaggagtt acgcaataaa ttgcggcaca ggccacagag tgtcgtgata
     1141 tcggcagggc gacctgccga atgcgctgaa atgtggttcc gattttatcg ggatcactca
     1201 ttcgtggtgg tgttgtgcgt cgcgcccttt tttctgccac cggatgtgag tatttctggc
     1261 gttctcaaaa acctgaggtt actgaaacca ggaatgtcag tagagcgtgt catcagtatt
     1321 gcaaatgcct ctggtgggtt ttcaggactg aaacatgcag aaaatttgcc agttatggac
     1381 agttactctg tctttatgaa tgaggtgaat aaccgcacaa agacaatcgt catgtcagaa
     1441 cgcttcccgg aaaagcaaaa gaaagtgttg tcgttgcttc ttgccggaca tagttgggaa
     1501 tacagtgcgc agtttctcaa aacggggata agacagatat ggcttgccga gcagtcgcta
     1561 aaaaagcgtt ggggaatacc ggacagcatg tcattgcggg aagccctgct gctgagcagc
     1621 aataaatttc acggtgatgg aaatgcatta gaaaaaacga atgccatgac attgcgtgaa
     1681 aacggaaata ccaacaggta ttccgtggtg gtaaatgcag ggacgcaggc tttatctctg
     1741 cataaatata aataagtggg aaattgaaat tagttaccag aatggagaat ttcttcatga
     1801 aaaacagtaa ggcattttac cgcagcgcat tagcgacagc tattgttatg gctctttctg
     1861 caccagcatt cgctgctgat aacgcggtat caactacacc ggttacattg gataaagata
     1921 agacaactct ggatcaagat gttgttatta acggtgataa aaagattaca gccgtaacaa
     1981 ttgaaacgtc agattcagat aaagacctta atgttacttt tggtggtcat gatattaccg
     2041 ccgcatcaac ggtaaaccaa gatttcgttg aaggtgtaaa agttagtggt aacaaaaatg
     2101 ttgtgattaa tgctacagac tccaccatca cagctcaagg tgaaggcacc tatgtccgga
     2161 ctgcaatggt cattgattca actggcgatg ttgttgttaa tggcggtaat ttcgttgcaa
     2221 aaaatgaaaa aggtagtgcg acagggatat ctctggaagg ggccaaggga aataatgtca
     2281 cgcttaatgg cacaaccata aatgctcaag gtaataagag ttccagcaac gcctccacgg
     2341 caatttttgc tcaaaagggt agcttattga atagttttga aggtgatgca accgacaaca
     2401 ttacccttgc tggctcaaat attattaatg gtcggattga aacaatagtt actgccggga
     2461 ataagacggg aattcataca gtcaacctga atattaagga tggctcagta attggggcgg
     2521 ctaataataa acaaacaatt tatgcctctg cttcggcaca aggcacaggt tcagcaacgc
     2581 aaaatttaaa tttgtctgtt gctgattcaa ccatctactc tgatgtcctg gccctttctg
     2641 aaagcgagaa ttcagccgct accacaacaa atgtaaacat gaacgttgcc cgctcttact
     2701 gggaaggtaa tgcttatact ttcaatagcg gcgataaagc gggtagtaat ctggatataa
     2761 atctttccga tagttcagtc tggaaaggca aagtttcagg ggcaggaaat gccagtgtat
     2821 ctctgcaaaa cgagtctgtc tggaatgtta cgggctcctc aactgttgat gctctggcag
     2881 taaaagacag tacggttaat atcacgaagg ctacagtcaa tactggcacg tttgcttctc
     2941 aaaacggcac tctgattgtt gatgcctctt ctgaaaacac tctggatatc agcggtaaag
     3001 cgagcggtga cttgcgtgtt tacagtgcgg gttcattgga tcttatcaat gaacaaacgg
     3061 catttatttc taccggcaaa gacagcactc taaaagccac aggcacaacg gaaggtggtc
     3121 tgtatcaata tgacctgaca cagggagctg atggtaactt ttatttcgta aaaaacacgc
     3181 ataaagcatc caacgccagc tccgtgattc aggcaatggc agcagctccg gctaacgttg
     3241 ctaatctgca ggctgacacg ctctcagccc gtcaggatgc tgtccgtctg agcgaaaatg
     3301 acaagggcgg cgtatggatt cagtactttg gcggtaaaca gaaacatacc accgcgggaa
     3361 atgcatccta tgacctggat gtaaacggtg taatgctggg tggtgatacc cgcttcatga
     3421 ctgaagatgg tagctggctg gccggtgtgg cgatgtcttc tgcgaaaggt gacatgacca
     3481 ccatgcagag caaaggcgac actgaaggtt acagcttcca cgcttacctg agccgccagt
     3541 ataacaacgg tatcttcatt gatactgctg cacagtttgg tcactacagc aacacggcag
     3601 atgttcgcct gatgaatggt ggcggtacca tcaaagctga ctttaacacc aatggtttcg
     3661 gtgcgatggt taaaggcggt tacacctggg aagacggtaa tggcctgttt attcagccat
     3721 atgccaaact gtctgcgctg acgctggaag gtgtggatta tcaactcaac ggcgtgaacg
     3781 ttcattctga cagctataac tctgtgctgg gtgaggccgg tacgcgcgtg ggttatgact
     3841 tcgctgtggg caacgcgacc gttaaacctt atctgaatct ggccgcactg aacgaattct
     3901 ctgatggcaa caaagtccgt ctgggtgatg agtctgtcaa tgccagcatt gacggtgcag
     3961 cattccgcgt gggtgcaggt gtacaggctg atatcaccaa aaacatggga gcatatgcaa
     4021 gccttgacta caccaaaggt gacgacattg agaatccgct acagggtgta gttggtatca
     4081 atgtgacctg gtaagaataa agcagtcggt tcccctgtcg actgttatac cccctgcagg
     4141 cagcagtatg ctgcctgcag gctataaata tccagtattc atgatgctgg ctttttgctg
     4201 atatttaacc cgaacattcc tgatacagac tattaattca tttatctgcg gcagatttta
     4261 ttttctggat atattatgag tgaacaaatc aaacaggata tcgatctaat tgagatttta
     4321 ttttatctga agaaaaagat tcgtgttatc ctttttatta tagctatctg tatggcaatg
     4381 gtgctgttgt ttctgtatat caataaagat aatataaaag tgagttacag cctaaaaata
     4441 aatcagacaa caccaggcat acttgttaac tgtgatagca ataataactt tgcctgtcag
     4501 accacaatga ctgaagatgt tattcagcga attactacat tttttcacac cagcccagat
     4561 gtcaagaaca gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct
     4621 gaagcggaaa tatctcgcgt gcaggcgtct attatcaatt ggtatgcgtc agaataccat
     4681 aatggcaggc aagttctcga tgagatacaa acaccttcag caattaacag tgagctttat
     4741 acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta
     4801 actataagct caccagaaat aaaaaataaa taccctgctg ccatttgcct gattctggga
     4861 ttttttctaa gcattgtgat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa
     4921 taccaacaaa actctgggca gtaataaaca gcattctgat agagagtgcc tgtataaagc
     4981 tgaagtgaca atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcac
     5041 tgtaaatatc ctggcattac taatgccagg atatttacag aagaggagca taaccatgac
     5101 ggaacggggt tcagtcaggt tgtccttaac gctattcaca acctcgtatg ccgccttcag
     5161 gtttatgtcc agacttcata tctctctcag caatcctcaa taatcagata caccgctttc
     5221 accgggccat gaaccccgac gactttgata agctcaatat ccgccgttga actagggccg
     5281 ctaatgatgt taatgcagga cggcatgcgt tcaccggcct gcgctttctg atgcaatttt
     5341 tctgcgagtt gcgctacacg cggcagtatt gtgcttttac gcaggataaa aagagaagat
     5401 tccgggagca ggctcaatga acgcccgcgc tcggcggcgg aaaaaagaac cacgcctccc
     5461 gattcggtta aaccatattc agcatacacg acacccactt tagcctgctc tgcctgcgag
     5521 atattctcgg cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt
     5581 tcgctaatcc ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc
     5641 agctctttac acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag
     5701 cgcgtcaaca taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta
     5761 agttgggtaa gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca
     5821 agtcgcaacg ggcgacccag tgcctgagca acgttattca aaaattcgcc tcgattatcc
     5881 attctttttc tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc
     5941 ttcaggaaga tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt
     6001 gccgccattg ataaaccagc ttgccgcatg agcgccggcc atcatcccga ctttccacaa
     6061 tcctggatga ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt
     6121 gatccctttt tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg
     6181 caccggacac acgctgtcac aagcagtgca taaagagcag gcgtagggta aatctttaaa
     6241 atctttatag ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga
     6301 gccatatcca tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca
     6361 gcgaatacag cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc
     6421 gacaataacc agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gcggtcctgt
     6481 cagccaggtg ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggcgat
     6541 caatacatct acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg
     6601 cgttttaggc agcgtggtac acattcgcgc attaccttca ttggtcacca ggcataccga
     6661 acctgtctct gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc
     6721 ttcgcggatt ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata
     6781 gcccagacgt tcgtggagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac
     6841 cacaacatga gatggcggat cttgatccag ctggagaata tattcaccca gatcggtttc
     6901 aatcacctga atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat
     6961 cgatttagat ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg
     7021 ggtagcgtct tctttggttt ttgcaaaata gacgtgaccg ccgttttgcg tcactttttc
     7081 tgagagctgg tacagataag cgtcgagatt actcagaaca tgatcacgta tctgggcggc
     7141 ccgatcgcgc cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc
     7201 aatacgctgc tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg
     7261 gatgcgtgtc ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc
     7321 tcatcaacac ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc
     7381 cactgatgtt tagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca
     7441 tcaggtgcgc aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga
     7501 acgtgccgcc aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac
     7561 gcacattttt cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac
     7621 aagatgggtg atacaccgct ctcccttgca aactggcacc tacatcgact acccctaatt
     7681 tattaacaat aaaagaggtg agatcctgca tacgcgcggc aacctttgcg gcacgtgatg
     7741 cccattcagg ttcatccgcc agatacatcg ggtaactttt tacggcatag gtgcaggagc
     7801 cagccggtga aataatggga tcatcgttat cctccagtgc ggcgatcaga tttttcatcc
     7861 ctggaatcgc ttctttgata taaccgctat tgatcgcagg ctgaccgcag catccctgtt
     7921 tctccgggaa atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca
     7981 ttcttgattt cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata
     8041 ctccattact tcatgcccat ttatgcccga tgtttccccc caccggggga tcaatcatca
     8101 ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt tgtatgacaa
     8161 atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt tcggcatcgc
     8221 tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt taaaaatggc
     8281 tacatccctt atacctattc agagctactt attcaggggt tattgagata tacactgatg
     8341 gtgtacaaaa aaatcaccga caataatcac gtatcaagta atagtcggga ttttatttaa
     8401 ttgaagcaat ttagtttgca tttttattta aaaactgagc tagagtccat cagaaaaaaa
     8461 tatataaagc ccaaaccacc gttggaaata gaaatagttg aaaggctcac aactcccgga
     8521 atgcggatta tctttgccct gatttctggc ggttttattc agggtgcaaa ctgtctgacc
     8581 ctttcccgat attctcccgg agtacaacca aactcgcgga caaacgcctt gtgaaaagat
     8641 gattcactgg catagcctac tgactcagcg atcaccacaa cagggagcat ttcccgggaa
     8701 aacatctggg ccgctatttg tagacgcaac tttgttaata cagccagcgg cgtggttccg
     8761 gaaacatcac ggaaaagctg ggcaaaactt gcccgggaca tgtgggcgat gctggccagc
     8821 gattcgacgg tccaggcgtg tcctggcatt tccagcattt gctgtattac cgcaccaaga
     8881 cgtggatgca gaagcaaact gagaatgttt ttctcagtat taacctgtgc aatccactca
     8941 cgcaccgcga gggtaaagaa tgtagcgcag atctggctac acagagcatc cattcccggc
     9001 attgccgatc tggattcctg ttgtaaaaac gggatcgcct ccctcagcca gttatattcc
     9061 acgctgtggt taaccggtgc taaaaacagc gtttccggca aagacgttaa aaaataacgc
     9121 gccgaatgtt gcagccgaag agtgccgcag acaatacagg tcgattcatt atcgacatga
     9181 ctcaggcgat gagctgaatt ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa
     9241 atctctcccg tcggcatttc cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata
     9301 accgacaatt ccccggcacc atgtggaagc tgccagtcac ttcctaacac gcaattctta
     9361 tcgatcgttc cttgtggagc gttaagcatc aacaaacggc taagggcatc catatcagac
     9421 tcctgagtgg tttttgatta caaacaccaa caatcttagt aaacaactaa tctaatacca
     9481 tcaaatagtt aatcatgatt tttgttttgt gattctgatt aagaaaaaga aaataccaga
     9541 cgataagaca aaatatcaag cgttgtgaag aaatgttatt tgctcttttg cgtctatagt
     9601 catgatgtca aatgaacgcg tttcgacagg aaatcatcat gaataaatat caggcagtga
     9661 ttattggttt tggcaaggct ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc
     9721 gtgtggctct catcgaacaa tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct
     9781 gcatcccaac caaaacattg gttcatgacg cacagcagca cacagatttt gtccgtgcca
     9841 tacagcgtaa aaatgaagtg gttaattttt tacgtaataa gaattttcat aatcttgcag
     9901 atatgcccaa tatcgacgtg atcaacggcc aggcggagtt tatcaataat catagcctgc
     9961 gtgttcatcg gcctgaggga aatctggaaa ttcatggcga gaaaattttt attaataccg
    10021 gtgcacaaac cgtggttccg ccaattcctg gaattaccac cacgcctggt gtatatgaca
    10081 gcaccggatt acttaatcta aaagaattgc ctgggcattt aggtattttg ggcggcggat
    10141 atattggcgt tgagttcgcc tctatgttcg ctaattttgg cagcaaggta accattttag
    10201 aagcagcttc gctgtttttg cctcgggaag atcgggatat tgctgataat atcgcgacga
    10261 ttttacgcga tcagggcgtc gatattatcc tcaatgccca tgtggagcga atcagtcacc
    10321 atgaaaatca agtgcaagtg catagcgagc acgcccaact ggcggtggat gcactgttaa
    10381 tagcttccgg tcgtcaaccg gctaccgctt cgttacatcc agaaaatgcc ggtgtcgcag
    10441 taaacgagcg cgggggaatt gtcgttgaca agcgattaca taccaccgca gacaatattt
    10501 gggcgatggg agatgttacc ggcgggctgc aatttactta catatcactg gatgattacc
    10561 gcattgtacg tgatgagtta ctgggtgaag gcaaacgtag tactgatgat cggaaaaatg
    10621 tgccttattc cgtatttatg acaccgccct tgtccagggt tggtatgaca gaagaacaag
    10681 ccagagagag tggtgctgat attcaggtgg tgacattgcc tgtagctgca attccgcgcg
    10741 ccagagtgat gaacgatacc cgtggggtat taaaagcgat tgttgataat aaaacccaac
    10801 gtatattagg ggcatcactg ctgtgtgttg actcccacga gatgattaat atagtgaaaa
    10861 tggtgatgga tgccgggctg ccttatagca tattacgcga tcagatattt actcatccgt
    10921 cgatgagcga atcactcaat gatctatttt cattaatcaa ataaactcaa aatcagacgc
    10981 cagaacaaat attctggcgt cccagagaaa agaatcttat taattccctg ttactctata
    11041 acttcttaat cacttcattg atggtatttt atatgtttaa aaaatccgtt ttatttgcaa
    11101 cactattatc tggcgttatg gcattttcca ccaatgcaga tgataaaata attctgaaac
    11161 atatcagcgt ctcgtcagta tcagcatcac cgacagttct ggaggatgcc attgctgata
    11221 tagccagaaa atataatgct tcatcctgga aagtcacatc gatgcgaatt gataataatt
    11281 caaccgcaac agcagtattg tataaataag gatgttcaca atggaaaaat acctgcacct
    11341 gttaagtcgg ggagataaaa ttggcctgac attgattcgt ctgagtattg cgattgtttt
    11401 tatgtggatt gggttattaa agtttgtccc ttacgaggca gacagcatta caccattcgt
    11461 cgcaaacagt ccactaatgt cgttctttta tgaacacccg gaagactata aacagtatct
    11521 gactcacgaa ggcgaataca aaccagaagc aagggcatgg caatcggcca ataataccta
    11581 tggtttttcc aacggtcttg gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa
    11641 tcctgtcaat cgctggttag gtttattggg agggctgatg gcatttacca caccgttggt
    11701 aacactctca tttttaatca ccaccccgga ggcatgggta cccgcattgg gtgacgctca
    11761 tcatggtttc ccttatttat ccggtgctgg tcgcttggta ttgaaagata ctctgatgct
    11821 ggcaggtgct gtcatgataa tggcagattc ggcgcgggaa attcttaaac aacgcagtaa
    11881 tgaatccagt tcaacgttaa aaactgaata ttgataatcc gaacactgtc tgttgttcac
    11941 tttttcaggc gtcaggcccc ctttctgatg atgaagcctg acgccatatt accggagata
    12001 tgacagagtg aagatcaata tcactcccct gttaaaagat tcttgtcgtc tcaggtaaaa
    12061 tacatgatta atatcaatca tgtattttta cagcaagaca accacgaaca aattcaggat
    12121 taaaatgttt taccgactcc ccaacataac ccaaatcaag cgaactaatt tgtgccattt
    12181 ctttctctgt cagtgagaaa tcccagatag caaaattttc ttcgatacgc tgttgatgtg
    12241 tcgatttcgg aataacgacc actccccgtt gaatattcca acgcagaatg acttgagaaa
    12301 tcgatttttg atgggcatct gcaatactct gaagcagatt attttcaaag ggtttatgtc
    12361 gtccaccacc taacggagcc catgcttcag gctgcacgtt ataatacttc atggttgcca
    12421 gcgcctctgg ttgggcaaaa tagggatgca actcgacctg gttgaccatc ggtttaactc
    12481 ttaccgtttc acaaaagttc gccagaacat gaggatagaa attggaaacc ccaatcgctt
    12541 ttaatttgcc ctcttcatag gcatcttcga gtgcacgcca cgcactgaaa taatcgcgca
    12601 tagcctgatg aaataaatag aggtcgaaat attctagtcc cgattttttt aatgatgctt
    12661 caatacctgc tgctgccgta tcctgattca acatatcctg cacccacaat ttagacgtta
    12721 taaataactc ttctcgggta cataaacctt cggatatggc ttcacgaacg gcttcaccga
    12781 cagcatcttc attaccgtat accgctgcag tgtcgatgag tcgataaccc gtgcggatag
    12841 cgttcaacac tgactgtttg cacacgtttt tatcggtaac ctgaaaaaca ccaaatccca
    12901 tcatcggcat tttcagattg ttacttaata cggaaaattc cactgtcagt ccctccatta
    12961 ggcacattag gcatgaacaa tgagtctacg ttaaaacgta acctcaaatt agtatgtgga
    13021 ttttgatatc acttatgcaa aaaattcatt aataatgtag gactgaaacc tctctatttt
    13081 cggggacaac gaagcagacg ctaccagtgc ttttgccttc gcccttgctg tttttgatac
    13141 acttagggcc cagggcataa cgaaaatgtg cgatatgaca aagaattaac ggagaatgtg
    13201 atgatcaggc agaagattct acagcagctc ctggagtgga ttgagtgcaa tcttgagcac
    13261 cctatttcaa tcgaagatat cgcacagaaa tctggctaca gcagacgcaa catccagctt
    13321 ctgttccgaa atttcatgca tgtgcctttg ggagaataca ttcgcaaacg aaggctttgt
    13381 cgtgccgcca ttcttgtccg gctcaccgcg aaatctatgc ttgatattgc actctctttg
    13441 cattttgatt cacagcagtc attcagccgc gaatttaaaa agttattcgg ctgctctccc
    13501 cgtgtatacc gccaccgtga ttattgggat ctcgcaaata tcttcccttc ttttttaata
    13561 cgtcaacagc aaaagacgga gtgtagatta gtcaactttc ctgagacacc tattttggca
    13621 attcatttaa atatgatatt gaagtgtcga ataaatcacc ggatgaagaa gtcaaactgc
    13681 gacgtcatca tttagccaga tgtatgaata attttaagac ggatatctat ttcgtctcca
    13741 cgtttgaacc gtcaacaaaa tcggtcgatt tgctcacggt tgaaactttt gctggtacgg
    13801 tatgtgaata tgctgacatg ccaaaagagt ggacaacgac ccgaggactt tatgcctctt
    13861 tccgttatga aggaaactgg gaaaattatc ctgactgggt gcgtaacatc tatctgatag
    13921 agttgcctgc cagggggtta gccagagtga acggcagcga tattgagcgc ttttattaca
    13981 atgaagattt cgtagaaaag gatggcaatg atgttgtttg cgaaattttt attcccgttc
    14041 gtccgattta gttggtcact atctcttatt gagttttatc cttgcctgat actatttaat
    14101 cagtatcagg caaggtattt agtgaataat ggcgttgaat atttcaacac cattattctt
    14161 tattagatcg taactttcat actattcaaa ttatggcatc tcctcctcgc cattttcagt
    14221 ctctgcagca acggcgttaa ttgtcacctt cacaggcgtc atttcgtacc cgccatacgt
    14281 cagtgcgttg aatgtgaaag tatagacacc tggcttgctg gtactaaatg tacgcgtgat
    14341 tttcccacct gagaaatggt cagccttcga cggcaggaat tgataatcct tctccgtcac
    14401 tccttccgga gcctcaatgt cgacccacat acttcctcct acgggattga ctcccgccat
    14461 cagggtaacc gtcattgttg cagtagtctt cccgtccgcc accagagaat ctacacgttc
    14521 tggagatgcg gcgagcaccg cgtttttatc atccgcgacg aatttagatt cagcagtttt
    14581 cgtcgatacc tgaccgttgg cctgttccac tttcgccgtg agtgtatatg tcgctgcgat
    14641 agtggtagag ccggtgaaaa cagcctgtcc ttgctcatta gttttcgccg tccctttagg
    14701 atccagaact aaattttccg agctggcagt cagcgtcacg tcgctatctg gctgactgct
    14761 taaaaatgtc ccggcatttg cggcaaacga cgcgacattt ttctccacgt tattatcagc
    14821 agttaccgta gtatttccca tgctcaaccg tggctgaacc gcatgctgcg cacgtgccgc
    14881 cattactggg gtaaaggtga cagcgagtgg aaaaagaacc tgaacagaga tatttgccca
    14941 cgccacgcag cgggccagaa ctgaataacg aaatcgtggt tgtttatgac ctgttttata
    15001 acgtgacatt gagataattt cctgatgaac gaattaacga acataggcag acacaaataa
    15061 tggcgtctgc ttgtcagaaa aaccggggat ttcccggttt ccttttccgc agaaaaggaa
    15121 atacctttat gtaagaaagt acaccgccac ctaatatatc cagtgcctgt agccactcat
    15181 cagg
//
LOCUS       DAZGBC010000118        15174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGBC010000118 DAZGBC010000000
VERSION     DAZGBC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.119"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(150..2429)
                     /gene="maeB"
                     /locus_tag="ACHK1J_003523"
     CDS             complement(150..2429)
                     /gene="maeB"
                     /locus_tag="ACHK1J_003523"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO7912748.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2718..3668
                     /gene="tal"
                     /locus_tag="ACHK1J_003524"
     CDS             2718..3668
                     /gene="tal"
                     /locus_tag="ACHK1J_003524"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7912749.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3688..5691
                     /gene="tkt"
                     /locus_tag="ACHK1J_003525"
     CDS             3688..5691
                     /gene="tkt"
                     /locus_tag="ACHK1J_003525"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7912750.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5786..6829)
                     /gene="ypfG"
                     /locus_tag="ACHK1J_003526"
     CDS             complement(5786..6829)
                     /gene="ypfG"
                     /locus_tag="ACHK1J_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO7912751.1"
                     /translation="MRYRNLLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6955..7530)
                     /gene="nudK"
                     /locus_tag="ACHK1J_003527"
     CDS             complement(6955..7530)
                     /gene="nudK"
                     /locus_tag="ACHK1J_003527"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO7912752.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7598..9577)
                     /gene="aegA"
                     /locus_tag="ACHK1J_003528"
     CDS             complement(7598..9577)
                     /gene="aegA"
                     /locus_tag="ACHK1J_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO7912753.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9783..11483
                     /gene="narQ"
                     /locus_tag="ACHK1J_003529"
     CDS             9783..11483
                     /gene="narQ"
                     /locus_tag="ACHK1J_003529"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO7912754.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11647..14760
                     /gene="acrD"
                     /locus_tag="ACHK1J_003530"
     CDS             11647..14760
                     /gene="acrD"
                     /locus_tag="ACHK1J_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO7912755.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14859..14918)
                     /gene="ypfM"
                     /locus_tag="ACHK1J_003531"
     CDS             complement(14859..14918)
                     /gene="ypfM"
                     /locus_tag="ACHK1J_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO7912756.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3583 a         4000 c         3932 g         3659 t
ORIGIN      
        1 cgacgcatga atataaataa aataatctcg agatatttta tatactgctt tttttattat
       61 tcgcaaactc agtaataagc tttactaata tattgcagcc aaaccgctta ccctgaatat
      121 tcagggtaag cgtgagagtt aaaaaagaat tacagcggtt gggtttgcgc ttcaaccacg
      181 gccagcgcca ccatgttgac gatacgacgc accgatgcga tcggcgttaa cacgtgaacc
      241 ggtttcgcca cgcccatcag caccggaccg acagtcacac cttccgagct ggaaacgcgc
      301 agtaagttgt aactaatgcg ggcagcttcc atgttcggca tcaccagaat attggcggaa
      361 cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg cttccaccag cgctgcatcg
      421 ccgtgcattt caccatcaat catcagttct ggtgcacgtt ccctgaccag ttccagcgcc
      481 tgacgcattt tgctcgacga cgggcagtca gaagaaccaa agttggagtg cgacaacaaa
      541 gcaacgcgcg gttcaatacc gaaacgacgg acagtttctg ccgccatcaa ggtgatctcc
      601 gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat cggcaataaa ggtgttacca
      661 ctcggcagca gcagcgcgtt cattgcacct gcggtgtgaa cgccatcgcg ataaccaaag
      721 acatttttca ccacgctaaa atgttcatga taatcgccca ccgtaccgca gatcattgca
      781 tcggcttccc cacgctgaac catgatcgcg ccgatcactg tcgggttact gatcagcgca
      841 cgctgcgcct gttcctgagt gacaccgcga cgcttcatga tctggaagta ttcggtccag
      901 tactctttaa agcgcggatc ggattcgtta ttgacgatct caaaatcaac gcccgctttg
      961 atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt tcggacgacc gataaggatc
     1021 ggtttcgcca gtcccagcgt taccagttcc tgagtggcat gcagaacgcg cgcctcttcc
     1081 ccttccggca gaactacgcg cttcggcgct ttgcgagcct gggagaaaat cggcttcata
     1141 aacaggttgg ttttgtaaac gaactcagtc agcttgtcga tgtagacgtc gaaatcagca
     1201 atcggacgcg tcgccacgcc cgactccatc gccgctttcg cgaccgcagg agcgatctta
     1261 acgatcaagc gcggatcaaa cggttttgga atgatgtatt ccggaccaaa gctcagatcc
     1321 tgatcgccat acgctgaagc caccacttcg ctctgttccg catgggccag ttctgcaatc
     1381 gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg ttgcgccaac gtccagcgcg
     1441 ccacggaaga tgaacgggaa gcacaggacg ttgttcacct ggtttggata gtcagaacga
     1501 ccggtgcaga tgatggcatc cggacgcact tctttcgcca gcggcggcag aatttccggt
     1561 tccgggttcg ccagcgccag gatcattggc gcacgagcca ttttcttcac catttcctgg
     1621 gtcagcactt tcgggccgga acagccgagg aaaatatccg cgccttcaat cacatcatcg
     1681 agggtacgtt tgccgtcatc caccaccgca tatgcggctt tggtttccgc catgtttggc
     1741 tcacggccct gatagataac gccttttgaa tcgcaaacca cgatgttatg tttttgcaga
     1801 cccagcgcga ccagcaggtt catacaggcg attgctgcgg cgcccgcgcc ggaaaccacc
     1861 atccgcacgt cagagatgtt tttctccacc acgcgcaagc cgttgaggat ggcggcagtg
     1921 ctgataattg ccgtgccgtg ctgatcgtcg tggaataccg gaatattcat ccgctcgcgc
     1981 agtttctgtt caatatagaa acattctggc gctttaatgt cttcgaggtt gatgccgccg
     2041 aaggtcggtt cgagcgcagc gacgacttca ataaatttgt ccgggtcgag ttcatcaact
     2101 tcaatgtcaa atacatcaat cccggcgaat ttcttaaaca gaacgccctt gccttccatc
     2161 accggtttgc ctgccagcgc gccaatgttg cctaacccca gcaccgccgt accgttagag
     2221 atcaccgcca ccaggttacc gcgcgcggta tatttgtagg cttttaacgg gtctttttcg
     2281 atttcaagac aaggtgcggc aacgcctggt gagtaggcca gcgccagatc gcgctgcgtt
     2341 gccagaggct tggttggaga aacctggatt ttccctggaa ctggaaattc atggaaatca
     2401 agtgcacttt gttttaactg gtcatccatt tggttgttcc tttcacgtaa cgttcacaaa
     2461 taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc acattattac cttgccggag
     2521 tctacagact ttgagcaagt ccaaactctc accattaata taatgttttg gtaataatcc
     2581 tataacactg atgttacctg cttaatccag caataccatg cctgtctgct atgctttttt
     2641 gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc actcatctaa cactttactt
     2701 ttcaaggagt atttcctatg aacgagttag acggcatcaa acagttcacc actgtcgtgg
     2761 cagacagcgg cgatattgag tccattcgcc attatcatcc ccaggatgcc accaccaatc
     2821 cttcgctgtt actcaaggct gccggattat cacaatatga gcatttaata gacgatgcta
     2881 tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca ggtggtcgca gcgtgtgaca
     2941 aactggcggt caatttcggt gctgaaatcc tcaaaatcgt acccggtcgc gtgtcaacag
     3001 aagttgatgc acgcctctct tttgataaag aaaagagtat tgagaaggcg cgccatctgg
     3061 tggacttgta tcagcaacaa ggcgttgaga aatcacgcat tctgatcaaa ctggcttcga
     3121 cctgggaagg aattcgcgcg gcagaagagc tggaaaaaga aggtattaac tgcaacctga
     3181 cgctgctgtt ttcttttgca caggcacggg cctgtgcaga agcaggcgtt tttctgattt
     3241 cgccgtttgt cgggcgtatt tatgactggt atcaggcacg caagccgatg gacccgtatg
     3301 tggtggaaga agatccaggc gttaaatcag tacgcaatat ctacgactac tataagcaac
     3361 accactatga aaccattgtg atgggcgcga gcttccgtcg caccgaacaa atcctcgcct
     3421 taaccggctg cgatcgactg actatcgcac cgaatttgct gaaggagctg caggaaaaag
     3481 tttcgccagt ggtacgtaaa ttaatcccac cttctcagac gttcccacgc ccagctccca
     3541 tgagcgaagc ggagttccgt tgggagcaca atcaggatgc gatggcggta gaaaaactgt
     3601 ctgaaggcat tcgtctgttc gccgttgatc aacgcaaact ggaagatctt cttgccgcca
     3661 aactataaac cagccacgga gtgttatatg tcccgaaaag accttgccaa tgcgattcgc
     3721 gcactcagta tggatgcggt acaaaaagcc aattctggtc atcccggcgc gccgatgggc
     3781 atggctgata ttgcagaagt gctgtggaac gattttctta aacataaccc taccgaccca
     3841 acctggtatg atcgcgaccg ctttattctt tccaacggtc acgcgtcgat gctgctctac
     3901 agtttgctac atctgaccgg ttacgacctg ccgctggaag aactgaagaa cttccgtcag
     3961 ttgcattcga aaacccctgg ccacccggaa atcggctata cgcccggtgt tgaaaccacc
     4021 accggtccac ttggacaagg tttggcgaac gccgtcggac tggcgatagc agagcgtaca
     4081 ctggcggcgc agtttaacca gccggatcat gaaattgtcg atcacttcac ctatgtgttt
     4141 atgggcgacg gctgcctgat ggaaggtatt tcccacgaag tctgttcgct ggcgggcacg
     4201 ctgggactgg gcaagctgat tggtttttac gatcacaacg gtatttcgat tgatggagaa
     4261 accgaaggct ggtttaccga cgatacggca aaacgttttg aagcctatca ctggcatgtg
     4321 atccatgaaa tcgacggtca cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa
     4381 agcgtgaaag ataagccgtc gctgattatc tgccgtacgg tgattggctt tggttcgccg
     4441 aataaagcag gtaaggaaga ggcgcacggc gcaccgctgg gggaagaaga agtggcgctg
     4501 gcacggcaaa aactgggctg gcaccatccg ccatttgaga tccctaaaga tatttatcac
     4561 gcctgggatg cccgcgaaaa aggcgaaaaa gcgcagcaga gctggaatga gaagtttgcc
     4621 gcctataaaa aggctcatcc gcaactggca gaagagttta cccgtcggat gagcggtggt
     4681 ttaccgaagg actgggagaa aacgactcag aaatatatca atgagttaca ggcaaatccg
     4741 gcgaaaatcg ccacccgtaa ggcttcgcaa aatacgctta acgcttacgg accgatgcta
     4801 ccggagctgc tcggcggttc ggcggatctg gctcccagca acctgaccat ctggaaaggc
     4861 tctgtgtcgc tgaaggaaga tccggcggga aattatattc actacggggt gcgtgaattt
     4921 ggcatgaccg ctatcgccaa cggcattgcg caccacggcg gctttgtgcc gtataccgcg
     4981 acattcctga tgtttgttga atacgcccgt aatgccgcgc ggatggcggc actgatgaaa
     5041 gcacggcaga ttatggttta tacccacgac tcaattggcc tgggcgaaga tggtccgacg
     5101 caccaggctg ttgagcaact ggccagcctg cgcttaacgc caaatttcag cacctggcga
     5161 ccgtgcgatc aggtggaagc ggcggtgggc tggaagctgg cggttgagcg ccacaacgga
     5221 ccgacggcac tgattctctc caggcagaat ctggcccagg tggaacgtac gccggatcag
     5281 gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca gcggcggtaa gccagatatt
     5341 attttgattg ccaccggttc agagatggaa atcaccctgc aagcggcaga gaaattagcg
     5401 ggagaaggtc gcaatgttcg cgtagtttcc ctgccctcga ccgatatttt cgacgcccag
     5461 gatgaggaat atagggagtc ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa
     5521 gcaggtattg ccgattactg gtacaagtat gttggtctga aaggggcaat tgtcgggatg
     5581 acgggttacg gggaatctgc tccggcggat aagctgttcc cgttctttgg ctttaccgcc
     5641 gagaatattg tggcaaaagc gcataaggtg ctgggagtga aaggtgcctg atggtgatcg
     5701 ccggatgctg attgccggat gcgacgctga cgcgtcttat ccggcctaca gcgtcttatc
     5761 cggcctacat gtcccgccat tttgtttaac gggtgatcca caacgtgggc caggcgtctg
     5821 gcccatgcca gttatcgcag gtgggttctg cggcgtaacg caccaggcga aaacgctgac
     5881 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     5941 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcatttgtct
     6001 cctgcccgtt gttgaacggc aaacgcaacc gaaccggacg cgaggctagt ggctttttac
     6061 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6121 tcagcgcttt atcatcagtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6181 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6241 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcaacgca ggcgctggcg
     6301 gtacgctgag cggcggttcg tcccctttct ttatccacgc ggtttcactg ccaacgcgct
     6361 tttgctgagc atcaataaac aacaacgccg cttttaagcc actcagagaa atggtctgat
     6421 tgccgtcgcg taaggtgatc gccttccctt cctgaatcat ctgcaaaaac gcggtgatgg
     6481 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaacg
     6541 ccaacggctc accatctaac agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6601 atttcaaccc gccgcgctca atacgtaaaa cggcatcggt atgcgccccg gcgctgcggc
     6661 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacgcag aagttttggt
     6721 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6781 acgtcggcaa caaagcgaaa aaaaagagaa ggagattgcg atagcgcatg gacggtacga
     6841 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgtggctca
     6901 atcggatttg tcggattgag ccaataataa acaacgaaat attattgtta tttttcagtc
     6961 cattaaatgt gacgtttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7021 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7081 atcttcgaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7141 gtggattagc tcagtcacac cgcctggcga catatacagt tcaaataatt tgcgcacttc
     7201 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7261 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7321 ggtagcgaca cggaactgac gaatcagaac cacggtcttt ttcttcgcgt tgtacaggag
     7381 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7441 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7501 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7561 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7621 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7681 ccagatccgc accacgcacg gcgtcaccac cagcgaagat tttcggattg gtggtctggt
     7741 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7801 gcgactccag ccacggcatc ccgtgcggat tgaagccaaa cgccataatc accgcgtcgg
     7861 ctggcatgac aaattcactg ccttccaccg gcactggacg cctacgcccc tgggcatccg
     7921 gctcgccaag acgcgtacgc aggaaacgaa tcccgcaaac gtgaccttgt tcattcagct
     7981 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8041 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8101 tcgcgccgtg acgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8161 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8221 tcacctgttt agtgttggcg atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8281 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8341 attgttccaa aagcgaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8401 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8461 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8521 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgcaccga
     8581 taatcgccac ccgcttgtcc actttggtga catggcttaa gtcaggacgc caacctttcg
     8641 ccaacgcctg atctgaaatg tagcgttcaa tgttgccgat agttaccgca ccgtgctcat
     8701 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8761 ccggcagggt gttggtcagg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8821 ccagttcaat ccactgcggt atatggttat gcagcgggca ggtccattca caaacgctgt
     8881 gctcgccgca tttaaggcag cgcgaggctt cccgttgtgc ctgatcggca cggaatggca
     8941 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9001 gcgcgggcgt tgcctgcatt tgctcgactt tactcattac cggcatttct tgcgccgcgg
     9061 tactggcatg ccacggttga tgttcctgac gtgcggtacg caagcggcgg gatttcgcca
     9121 tgcctgagag tgcggcgtca gtgaccagct gcagcgcgtc cgccgggcaa ttctcgacac
     9181 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gctttaactt
     9241 tgcccgctgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9301 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9361 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9421 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatga tgttggctca
     9481 ggacatgttg ctcatcattg tgagccatga cacaagcgat ttcacaagca tgacaaccca
     9541 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9601 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa tcggcaatgt
     9661 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9721 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9781 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9841 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
     9901 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
     9961 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt atttcagcag gcactgcatt
    10021 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10081 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10141 ggtatcaggc caatatcaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10201 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggcatcg
    10261 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10321 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccaccgc
    10381 tggataccag cctgccgaat gagcttggtc tgcttgcaaa aacctttaac cagatgtcga
    10441 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10501 tccacgaggc caagcgtcgt ctggaggtgt tgtatcaatg ttcgcaggcg ctgaacacca
    10561 gccagattga tgtgcattgt ttccgccata ttttgcagat tgttcgcgat aatgaagcgg
    10621 ctgaatatct ggagttaaat gtcggtgaaa actggcggat tagcgaaggg caaccaaacc
    10681 cggaattgcc gatgcagatt ttaccggtga caatgcaaga gacggtttac ggcgaactgc
    10741 actggcaaaa tagtcacgtt tcatcatcag aaccgctgct taacagcgtt tcgtcgatgc
    10801 tgggacgcgg tttgtacttt aatcaggcgc agaagcattt tcagcagtta ttgttgatgg
    10861 aagaacgtgc aaccatcgcc cgcgaattgc acgactcgct ggctcaggta ctttcttact
    10921 tacgtatcca gttgacgtta ctgaagcgtt cgataccgga agataacgcc accgcacaaa
    10981 gtatcatggc cgatttttcc caggcgttga atgatgctta tcggcagtta cgcgagctgt
    11041 tgactacttt tcgcctgacg ctgcagcagg cggatctccc ctccgcgttg agggaaatgc
    11101 tggatacgtt acaaaatcaa accagcgcca aactgaccct cgactgccgt ctgccaaccc
    11161 tggcactgga tgcgcaaatg caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga
    11221 atgcgatgaa gcacgccaac gccagcgaaa tcgccgtcag ttgcgtcacc gcgccggacg
    11281 gcaatcacac ggtttatatc cgtgataacg ggattggtat cggtgaaccg aaagaacccg
    11341 aaggtcatta tggtctgaat atcatgcgcg aacgcgcgga acggctaggt gggacgctga
    11401 ctttttcaca accttccggc ggcggtacgt tagtgagtat tagctttcgc tctgcggagg
    11461 gtgaggaaag tcagttaatg taatgcctcc tactgaccaa agaatgcttg cacttaaggt
    11521 tcagtataaa agggcatgat aatttacatt aactcctttt tttctccacg attggctcgt
    11581 accttgccgc tacagtgaag caagtcaagc ctacaacgat acgcagaaac acgaggtcct
    11641 cttttaatgg cgaatttctt tattgatcgc cccatttttg cctgggtgct ggcaatcctg
    11701 ttgtgtctga caggtaccct ggcgattttt tcattgcccg ttgaacaata ccccgatctc
    11761 gcgccaccga atgtgcgagt gaccgctaac tatcccggcg catcggccca gacgctggaa
    11821 aacaccgtga cccaggttat cgagcaaaat atgactggcc tcgataatct catgtatatg
    11881 tcatctcaga gcagtggcac cggtcaggca tctgtcactt taagttttaa agcaggcacc
    11941 gatccggacg aagccgtgca gcaagtacaa aaccagctgc aatcagccat gcgaaagtta
    12001 ccgcaggcgg tgcaaaatca gggcgtgacg gtgcgtaaaa ccggcgatac caacattctg
    12061 accattgcct tcgtctctac cgatggttcg atggataaac aggatattgc tgattatgtt
    12121 gccagtaata ttcaggaccc gttaagccgc gtgaatggcg tcggggatat cgatgcctat
    12181 ggttcgcaat attccatgcg tatctggctg gacccggcga aactcaacag tttccagatg
    12241 acggctaaag atgtcactga tgccattgag tcacagaacg cgcagattgc ggttgggcaa
    12301 cttggtggta caccttccgt cgataagcag gcgctcaacg ccaccattaa cgcccagtca
    12361 ctgctgcaaa caccagaaca gttccgcgat atcaccttgc gggtgaatca agacggctca
    12421 gaggtaaggc tgggcgatgt cgccaccgtc gaaatggggg cggagaaata cgattatctt
    12481 agccgcttca atggtaagcc agcctccggg ctgggggtaa aactggcctc cggcgctaac
    12541 gaaatggcga cagcggagct ggtgctcaat cgtctcgacg agctggcgca gtatttcccg
    12601 catggactgg aatacaaggt ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa
    12661 gacgtggtga aaacgctgct ggaagctatc gctctggttt tcctcgttat gtatctgttc
    12721 ctgcaaaact tccgcgccac gctgataccc actatcgccg tgccggtggt gttgatggga
    12781 accttctccg tactttacgc cttcggttac agcgtcaaca ccttaaccat gttcgccatg
    12841 gtgctggcga tcggtctgct ggtggatgat gccatcgtgg tggtggaaaa cgtcgaacgt
    12901 attatgagtg aggaaggact cactcctcgc gaagccacac gtaaatcgat ggggcagatc
    12961 cagggggcac tggtcgggat tgcgatggtg ctttcggcgg tatttgtacc aatggccttc
    13021 ttcggcggca ccaccggtgc catctatcgc cagttctcta ttaccattgt tgcggcgatg
    13081 gtgctgtcag tactggtagc gatgatcctc actccggctc tgtgtgccac gctacttaag
    13141 ccactgaaaa aaggtgagca tcacgggcaa aaaggctttt ttgcctggtt taaccagatg
    13201 tttaaccgca acgccgaacg ctacgaaaaa ggggtggcga aaattctcca ccgtagcctg
    13261 cgctggattg tgatttatgt cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg
    13321 ccgacgtcgt tcttaccgct ggaagaccgt ggcatgttta ctacctcggt acaattgccc
    13381 agcggttcaa cccaacaaca gaccctgaaa gtcgttgagc aaatcgagaa atattacttc
    13441 acccatgaaa aagacaacat catgtcggtg tttgccactg ttggttctgg ccctgggggt
    13501 aacgggcaaa acgtggcgcg aatgtttatc cgcctgaaag actggagcga acgcgacagt
    13561 aagaccggca cctcatttgc cattatcgag cgtgcaacaa aggcgtttaa ccaaattaaa
    13621 gaagctcgcg ttatcgccag cagcccgcca gcaattagcg gtcttggtag ttctgcaggt
    13681 tttgatatgg agttgcagga ccacgctgga gcaggtcacg atgcgctgat ggcagcacgt
    13741 aaccagttgc tggcgctggc ggcggaaaac ccggagctaa cccgtgtgcg ccataacggc
    13801 ctcgatgaca gtccgcagtt gcagattgat atcgaccagc gtaaagctca ggcgctgggc
    13861 gttgctatcg acgatattaa cgacacactg caaaccgcct ggggttcgag ctatgtgaat
    13921 gactttatgg atcgcggtcg cgtgaagaaa gtctatgtgc aggcagctgc gccgtatcgc
    13981 atgctgccag atgacatcaa tctctggtat gtccgaaata aagatggcgg catggtgccc
    14041 ttctctgctt tcgcgacctc acgctgggag acaggttcgc cgcgtctgga acgctataac
    14101 ggttattctg cggttgagat tgttggggaa gccgcaccgg gggtcagtac cggtacggcg
    14161 atggatatta tggaatcgtt agtgaagcag ttgccaaacg gctttggtct ggagtggacg
    14221 gcgatgtcgt atcaggagcg gctttccggc gcgcaggctc cggcgctgta cgctatttcc
    14281 ttgctggtgg tattcctgtg tctggcggcg ttgtatgaaa gctggtcggt accgttctcg
    14341 gtaatgctgg tcgtgccgct gggggtaatc ggcgcgctgc tggcaacctg gatgcgcggg
    14401 ctggaaaacg acgtttactt ccaggtgggc ctgttaacgg tcattggttt atcggcgaaa
    14461 aacgccatcc tgatcgtcga gtttgctaac gagatgaacc aaaaaggcca cgacctgttt
    14521 gaagcgacgc tccacgcctg ccgtcagcgt ttacgcccga ttctgatgac ctcgctggca
    14581 tttatcttcg gcgtattgcc aatggcaacc agcacgggtg caggttccgg tggtcagcat
    14641 gcggtgggta ctggcgtaat gggcgggatg atttcagcca ctattctggc tatttacttc
    14701 gtgccgctgt tctttgtgct ggtgcgccgc cgcttcccgc tgaagccgcg cccggaataa
    14761 gcaataaaaa aggcgacatg ctaatgtgtc gcctttttca actttccgat taagaacctg
    14821 ctcagcgggt tcttgctgtt tgtactttgt ctcaggaatt acttacgaag cataacttcg
    14881 ataaagtctt tccagttccc cagttcacgt tcaatcataa caacctctct tataattatg
    14941 ggtattctac ggaaacaata taccgtggtg aagctaattt actcgattgc tgcgatgact
    15001 acctccgggg gacaaacctt atgtaaatac tatggtcctg cggtgataat ttgtatgtga
    15061 tacacagcaa catttcgaga tattcatacg gcatctaata ctgatttaat tctggttaaa
    15121 atacagacag ataacaagat gaatattctt aatgtttacg ttaaaaatgt ttaa
//
LOCUS       DAZGBC010000119        15042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGBC010000119 DAZGBC010000000
VERSION     DAZGBC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.120"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..364
                     /gene="yjbE"
                     /locus_tag="ACHK1J_003532"
     CDS             122..364
                     /gene="yjbE"
                     /locus_tag="ACHK1J_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO7912757.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            478..1116
                     /gene="yjbF"
                     /locus_tag="ACHK1J_003533"
     CDS             478..1116
                     /gene="yjbF"
                     /locus_tag="ACHK1J_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO7912758.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1113..1850
                     /gene="yjbG"
                     /locus_tag="ACHK1J_003534"
     CDS             1113..1850
                     /gene="yjbG"
                     /locus_tag="ACHK1J_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7912759.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1850..3946
                     /gene="yjbH"
                     /locus_tag="ACHK1J_003535"
     CDS             1850..3946
                     /gene="yjbH"
                     /locus_tag="ACHK1J_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7912760.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGVDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3993..4271)
                     /locus_tag="ACHK1J_003536"
     CDS             complement(3993..4271)
                     /locus_tag="ACHK1J_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912761.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4272..4415
                     /locus_tag="ACHK1J_003537"
     CDS             4272..4415
                     /locus_tag="ACHK1J_003537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912762.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4541..4951
                     /gene="psiE"
                     /locus_tag="ACHK1J_003538"
     CDS             4541..4951
                     /gene="psiE"
                     /locus_tag="ACHK1J_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO7912763.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4995..6470)
                     /gene="xylE"
                     /locus_tag="ACHK1J_003539"
     CDS             complement(4995..6470)
                     /gene="xylE"
                     /locus_tag="ACHK1J_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO7912764.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6842..7732)
                     /gene="malG"
                     /locus_tag="ACHK1J_003540"
     CDS             complement(6842..7732)
                     /gene="malG"
                     /locus_tag="ACHK1J_003540"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO7912765.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7747..9291)
                     /gene="malF"
                     /locus_tag="ACHK1J_003541"
     CDS             complement(7747..9291)
                     /gene="malF"
                     /locus_tag="ACHK1J_003541"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO7912766.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9445..10635)
                     /gene="malE"
                     /locus_tag="ACHK1J_003542"
     CDS             complement(9445..10635)
                     /gene="malE"
                     /locus_tag="ACHK1J_003542"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO7912767.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11000..12115
                     /gene="malK"
                     /locus_tag="ACHK1J_003543"
     CDS             11000..12115
                     /gene="malK"
                     /locus_tag="ACHK1J_003543"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO7912768.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12187..13527
                     /gene="lamB"
                     /locus_tag="ACHK1J_003544"
     CDS             12187..13527
                     /gene="lamB"
                     /locus_tag="ACHK1J_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO7912769.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13770..14690
                     /gene="malM"
                     /locus_tag="ACHK1J_003545"
     CDS             13770..14690
                     /gene="malM"
                     /locus_tag="ACHK1J_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO7912770.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVAGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14919..>15042
                     /locus_tag="ACHK1J_003546"
     CDS             14919..>15042
                     /locus_tag="ACHK1J_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912771.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNGVPGLN"
BASE COUNT         3709 a         3874 c         3960 g         3499 t
ORIGIN      
        1 tatcctaaaa ccccatctct aattccccgc gccgtaattc gcatgcttta gttgtgtata
       61 ctcgatcccg cccgaaatgt ttttgggtaa atctccattc attcaatgaa gggaaattgt
      121 tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga cttctgcgtg
      181 ggctgcacct gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg
      241 tagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc
      301 aaccggtggc ggtgatggtt ctaataccgg gaccacaaca accacgacca ccagtaccca
      361 gtaataaagt atgtatcccc aaaataattc gagtcatcgc atctgtggct tgaagtatga
      421 agggatttac cataaccaca ctccggtgtg gttattctgc ccctctggag aagagtcgtg
      481 aagcgacctg cattcattct tatctgcctg ctattacagg cctgttcagc cacgactaaa
      541 gagctgggca attcactgtg ggacagtctg ttcggtacgc caggtgtaca gctgacggac
      601 gatgatattc aaaatatgcc ctacgccagc cagtacatgc agcttaatgg cgggccgcag
      661 ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa aatgggtcac tcaggatcag
      721 gccactctcg tcacacaaca tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg
      781 attgaagtga ataaccttgc cgccgacccg ctgattaaac ccgcacaaat tgttgatggt
      841 gcaacatgga cgcgcacgat gggctggacc gagtaccagc aggtacgcta cgccaccgca
      901 cgctcagtct tcaaatggga tggcaccgat accgtcaaag tcggcagcga tgaaaccccg
      961 gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc gctggcataa ccgctattgg
     1021 atcgacagcg aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg
     1081 gtgaaaacca ccctcatcaa ggcggcaaaa caatgatcaa acaaactttt gtcgcgttgc
     1141 ttttgagtgt gggagcgtca tcggtcttcg cggcaggaac cgtcaaggtg ttcagcaatg
     1201 gcagcagtga ggccaaaaca ctaacaggcg cagagcattt aatcgatctg gtaggccaac
     1261 cgcggctggc aaacagctgg tggcccggtg cggtgatcag cgaagagctg gcaacggcgg
     1321 cagcattgcg tcagcagcag gcgttgctga cacggctggc agaactggcc gcagactcca
     1381 gcgccgacga tgccgctgcg attaacgcct tacgccagca aattcaggcg ttgaaggtga
     1441 cgggcaggca aaaaatcaat cttgatcccg atatcgtccg cgttgctgaa cgcgctaacc
     1501 cgccgttgca gggcaactac acgctgtggg tcggaccacc accgtccacg gtcacgttgt
     1561 tcgggcttat cagccgtcct ggcaatcagc cattcactcc cggtcgcgac gtggcgagct
     1621 atctctccgg gcaaagtctg cttagcggtg cggatcgtag ctacgcgtgg gtggtttacc
     1681 cggacggacg cacacaaaaa gcgccggtgg cttactggaa caagcgtcac gtagagccga
     1741 tgcccggcag cattatttat gttggcctcg cggactccgt ctggagtgag atgcctgatg
     1801 cccttaacgc cgacattctt cagactctga cgcagcggat acctcaataa tgaaaaaaag
     1861 acatctgctt agcttactgg cgctgggcat tagcacagct tgctacggcg aaacatatcc
     1921 tgcgcccatt ggtccgtcgc agtcggattt cggtggcgta ggattattac aaacgcccac
     1981 cgcgcgtatg gcacgggaag gggagttgag tctgaactat cgtgataacg atcagtaccg
     2041 ttattactca gcttcagtgc aactcttccc gtggctggaa acaacgctgc gctacaccga
     2101 cgtgcgcacc cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga
     2161 taaagccttc gatctcaaac tgcgtttgtg ggaagagagt tattggctgc cgcaagtggc
     2221 ggttggtgcg cgggatatcg gcggtacggg gctgtttgat gcggaatatc ttgttgccag
     2281 caaagcctgg gggccgttcg attttacgct cggcctgggc tggggatatc tgggcaccag
     2341 cggtaatgtg aaaaatccgc tctgttcagc cagtgataaa tattgctatc gcgataacag
     2401 ctacaaacag gcgggatcta tcgacggtag ccagatgttc cacggtcctg cctcactgtt
     2461 tggcggcgtg gaataccaga cgccctggca accgctgcgc ctgaaactgg agtatgaagg
     2521 caataattat cagcaggatt ttgccgggaa gctggagcaa aaaagtaagt ttaacgtcgg
     2581 ggcgatttat cgcgttaccg attgggccga cgttaacctt agctatgaac gtggcaacac
     2641 ctttatgttt ggcgtcacgc tgcgcaccaa ctttaacgat ctgcgcccgt cttacaacga
     2701 taacgcccgc ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc attcggtggt
     2761 ggcgaatcag ttaacgctgt tgaaatacaa tgccggactt gccgatccgc agatccaggc
     2821 gaaaggcgat acgctgtatg ttaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg
     2881 gatcatccgt gctaatcgga tcgtgatgaa tgatctgccg gatgggatca agacgatccg
     2941 cgttacggaa aatcgcctta acatgccgca ggtgacgacg gaaacggatg tcgccagcct
     3001 gaaaaatcat ctcgccggag agccgttggg ccacgaaacg aagctggcgc aaaaacgcgt
     3061 cgagccagtg gttccgaagt ccaccgagca gggctggtat atcgacaaat cacgctttga
     3121 tttccatatc gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact tttacatgta
     3181 tcagctcggc gtgatgggaa cggcagattt gtggctgacg gaccatctgc tgaccaccgg
     3241 cagcctgttc gctaaccttg ccaacaacta cgacaagttt aactacacca atccgccgca
     3301 ggactcgcac ttaccgcgcg tgcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta
     3361 tgtgaataac ctgcaagcca actacttcca gcatctgggc aacggcttct acggtcaggt
     3421 ctacggcggc tatctcgaaa ccatgtttgg cggtgcgggg gcagaagtgt tgtatcgccc
     3481 gctggacagc aactgggcgt ttggtgtgga tgccaactac gttaaacagc gcgactggcg
     3541 tagtgcaaaa gatatgatga aattcaccga ctacagcgtg aaaaccggac atctgaccgc
     3601 ctactggacg ccatctttcg ctcaggatgt gttagttaaa gccagcgtcg ggcagtatct
     3661 ggcaggggat aaaggcggca cgctggagat cgccaaacgc tttgatagcg gtgtggtggt
     3721 gggtggctat gccacgatca ctaatgtttc gaaagaggag tacggcgaag gggacttcac
     3781 caaaggcgtg tatgtctctg taccgttgga tctcttctcg tctggcccga cacgcagccg
     3841 tgcggcaatt ggctggacgc cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt
     3901 ccagttgtat gacatgacca gcgaccgtag cgtcaatttc cgctaagtca tgggaaaggt
     3961 gccagttttc gcactcactg gcaccttcat tcttaatggc ctgtcgagcc atcaggaacg
     4021 cagttcaccc ccggtttgcg atctggtgtg taaaggcgaa aacattctgt cttcctgtcc
     4081 gtcgatatcg gttgcaggtc tggttgcctg agcgactggg ccatcattgg gctggagaca
     4141 ttgctttgcg gtaggtcggc tttatcagca gtaccgagcg gaccagcata agcaggaaga
     4201 acagagactg ataacatcaa agcagcaaaa taaggcttca tttttaccac ctttatcagg
     4261 ttacgtttca tttgttccag aggaacattg tcgatttttc gcgcattgct ggtggctggg
     4321 aatcacctga atgggtgatt tttgaattac cggctttggt gcggtttgtc ttgccggatg
     4381 cgccgccagg cgcggcttat ccggcctacg ggtaggtata tccggctttg gtggaggcgc
     4441 gctccaaatc caggttgaac aaaacataca caaaaaatat agatctccgt cacatttttg
     4501 cgttatacag gaagctcgcc actgtgaagg aggtactgct atgacgtcac tctctcgtcc
     4561 gcgcgtggag tttatctcca ccattttgca gaccgtactc aatcttggcc tgttgtgcct
     4621 cggcctgatt ttggttgtct tcctcggcaa agaaacggtg catctggctg atgtgctatt
     4681 cgcgccagaa caaaccagca aatatgagct ggtagaagga ctggtggttt actttctcta
     4741 tttcgaattt atcgcgctga ttgtgaagta ctttcagtcc ggttttcact tcccgctacg
     4801 ttactttgtc tatattggga tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc
     4861 gccactggat gtgttgatct actcggctgc gatcctgtta ctggtgatca ccctgtggct
     4921 gtgtaattcg aaacggctga agcgggagta aaaagacagc acgccgaact ggcgcggcgt
     4981 gctggacagg aagattacag cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag
     5041 agggcttcca gctcctcaag ggttttacct ttggtttccg ggacaaattt ccacataaat
     5101 agtgctgcca gaacgcccat acacccgtaa atccagtagg agaaaccgtt gtggaaatgg
     5161 gccaccagcc aggagttttt gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc
     5221 agccactggg ccgccaccgc gattgccagc gctttaccac gaatagcatt cgggaagatt
     5281 tccgacagca gtacccagca taccggaccc caggacatgg caaaggcggc aacatagaac
     5341 agcatcgaca gaagcgccac aatacccggt gcctgagtgt aaaacgcggt accgaggcta
     5401 aacataccga ttgccattcc gagtgcgccg ataatttgca gtggcttacg accaaattta
     5461 tccaccgtca taattgccag aacggtgaag gtgaggttga taactccgac aataatggtc
     5521 tgcaacagcg cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac
     5581 agcaccacat tgatgccgac aaattgctgg aagatggaga gcataacgcc gatgacaatc
     5641 acgcccacgc caaacatcag cagacgacca ccggttttgc ggccatgatc cagggagtgt
     5701 ttaatttcct gtactgcctg agttgcaagc gtgttgccca taattttgcg caggatacct
     5761 tccgcctgtt cttgcttgcc gcgtgacatc agccagcgag gactttctgg cacggtatac
     5821 agcagcatta agaacagcag tgcagggata cattccgagg caaacatata acgccagccg
     5881 tcagtattca gccagctggc atcaccggaa cgggcaataa aatagtttac gcagtaaact
     5941 aaaagttgcc cgaaaataat cgcaaactgg ttaaaagaga ccagtttccc gcgaatatga
     6001 gctggagcca gttccgcaat atacatgggc gagagcattg aggctaaacc aacgccaata
     6061 ccgccaataa tgcgataaat aacaaattcc gggacataac ctgccagata aacaggcact
     6121 gtgttgtccg ggtttataga ggtaaaacca agttctggcc aggcagaacc tacaccagaa
     6181 ataaaaaaca ggacagcagc aatcttaagt gaatcacgac gaccgaagcg gctactgcaa
     6241 taaccaccga gggcaccacc gatgatgcac ccaatcagag cgctggctac gcaaaaccct
     6301 aacagggagt tggcagcgga ttcacttaag ttttgtggag caacaaagac ggtattgagt
     6361 gactcaacag taccggaaat aacggcggtg tcgtagccaa ataataaacc acctaatgta
     6421 gcgactaagg taatcgaaaa tatataactg gaattatact gggtattcat tcagacctgc
     6481 cttagaccat tctgatcttt tatcagtgca ttgatgttgg aattgtcagg cagttaaggc
     6541 aattatcatt ttttacaatg cgtttacgta atgtcttttc tgtgatctta attgtgataa
     6601 ttatccaaaa ttgataaaaa caatactatt gccgtgactc agagcacgaa agagaattat
     6661 cgtaagtggg aaaacaaata acgtaaaaat aataagttct atgatggaaa tattaaccgg
     6721 cgaacgattc agattgcaga cgaaagaaaa aaaggcgctc cgtggagcgc cgaataacag
     6781 tcacaagttg ggataacgta agttgagggt gcagcggcat aacattggca gaacaacatc
     6841 tttaaccttt cacaccacct gccgtcaggc cgttgaccag ccagcgttga gctagcaaga
     6901 agacgatggt gatcggtaat gcagacatca cggcagcggc ggcaaagtca ccccacaggt
     6961 agttttgcgg gttgaggtat tgctgcatcc ccacggccag ggtgtagctg tttacgtcac
     7021 gcagtaacag cgatgcgacc ggaacttcag taatggcagc gataaacgac aggatgaaca
     7081 ccaccgccag aatcggtact gacagcggca acaggacaag gcggaaggcc tgccacggtg
     7141 tcgcaccatc cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg aaatagcctt
     7201 tgatggtcca gacgtgcagc gcaatcccac ccagatacgc gaaaattacg ccgccgtgag
     7261 tattcaggcc aatgaatgga atgtactcac ccagacgatc aaacaacgca tacaacgcga
     7321 ccagtgaaag tactgccggg aacatctgga aaatcagcat tcctttcagc agcgtcgctt
     7381 tgcctggaaa gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc
     7441 caatcgcgga aatcccggcg acctttaccg agttccacag ccacagcagt accgggaatg
     7501 gcggtggcgt aatgcgacca tcagcctgtt caacgctaaa acctaacgcc agtttccagt
     7561 gatcccagga gatttgctcc gggatcaggc taccggtcgc aaagttcccc tggcgcagtg
     7621 agatggcgac gaccatcagc agcgggaaca taatcgccgc gataaaaagt agcagtagca
     7681 ggtgagtaat aaacaaacgt gctttttgcg atttcggttg gaccattgcc atttttgtta
     7741 tctcccttaa tcaaacttca ttcgcgtggc tttcaggttc actatcgcca gcgcacctac
     7801 cagcaggaag atcagcgtgg caattgctgc tgccagaccg aagtcctgac ccccgccgcc
     7861 ttcaaaagcg atgcggtagg tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt
     7921 cgtgccaaga cgatccgggc cgccgttggt taacagttga atcagcacga agttgttaaa
     7981 gttaaaggcg aagctggcga tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt
     8041 aatcttaaag aagttctgga acggacctgc accatccatt gctgaggctt catacaaatc
     8101 gtccggaatc gctttcagca agcccatgca gaggatcatc atgtacggat aacccagcca
     8161 ggtattgacg ataatcagca tcgttcgggc ggtggtcgga tcgctgaacc aggcgggctt
     8221 cacgccaaac agcgcgctca acatcatgtt gatctcaccg aagctctggt taaacaaccc
     8281 tttgaaaatc aagattgaaa tgaacgatgg caccgcgtag ggcagaatca gcaggacgcg
     8341 atagaccgct ttgccgcgca acgcttccca ctgcaccaga cacgccagaa ccatgccgac
     8401 tgccactgtt aaaaagacag tgatcagcga gaacaccacg gtccagacga aaatggcgag
     8461 gaacggtttc tgaatgcctt cgtcggtgaa gacgcgggta aagtttttcc agccagtagt
     8521 cacggtgtaa ccggggctta gcttttcatc gccccagttg ccgtcggcgg taatggactg
     8581 gtaaaagcca atttggttat tcggacgata tttcacgccg ctctgattat tcgtcaacgt
     8641 gccgtcaccg tcgagtgtgt agagcggctg cgtgccagaa aactggcgca gggagctcat
     8701 catcactttg ttgccatccg gcagaatggc ggtaatgtca ctcagcgcct gacgattctg
     8761 ggtaatcacg cgtagattcg cgcgttcgcc ttcgggctgg gcggtcgttt ctttcagttg
     8821 cagtttttgc tctccgccaa atttaaaagc gtcggagagg taatttttgc cggtttcgcc
     8881 gtcgctgagc gccagttgcc actcatcgcc cgccgggtaa agaccaaagt tataggtttt
     8941 gcctgcttgc caggagcgat ctaacaacac ttcctgtgca cgttcaaaag tcagctggtt
     9001 agtgctgctg tagtttgtga aggcaatggc gatggtgcag accagaggga agaggacgaa
     9061 taatcccatt ccagccattc ccgggtaaac atagcgccag gcgtaggctt tacgattggc
     9121 gaaaatatac agccccgctg aactcaatat cagcgtggta atggcgaaca ggtattcccc
     9181 ttgtgcgtac attaaaacaa caaggtaacc caccagcagg ccgagcagac ctaacactga
     9241 ccatttcagc gcgtcgcttt gccaccaatg tttcttttta atgacatcca tggggttctt
     9301 cctcattcca ggacggataa ggctttcacg ccttatccga caacaactgc ctgatgcgac
     9361 gctgacgcgt cttatcaggc ctacatacgt ttcggttttg taggccggac aaggcgttca
     9421 cgccgcatcc ggcatttcac agcattactt ggtgatacga gtctgcgcgt ctttcagggc
     9481 ttcatcgaca gtctgacgac cgctggcggc gttgatcacc gcagtacgca ctgcatacca
     9541 gaaagcggac atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatggt
     9601 ggctgcaata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ctacggcacc
     9661 cagcggtttg tctttattaa ccgcttccag accttcgtca gtcagcagat agttttcgag
     9721 gaactctttc gccagctctt tgttcggact ggcggcgtta atacctgcgc tcagcacgcc
     9781 aacgaacggc ttggatggtt gacccttgaa ggtcggcagt accgttacac cataattcac
     9841 tttactggtg tcgatgttgg accatgccca cgggccgttg atagtcatcg ctgtttcgcc
     9901 tttattaaag gcagcttctg cgatggagta atcggtgtct gcattcatgt gtttgttttt
     9961 aatcaggtca accaggaagg tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc
    10021 tttaatgtcg tacttgccgt tttcatactt gaacgcataa cccccgtcag cagcaatcag
    10081 cggccaggtg aagtacggtt cttgcaggtt gaacatcagc gcgctcttac ctttcgcttt
    10141 cagttcttta tccagcgccg ggatctcttc ccaggttttt ggcgggttcg gcagcagatc
    10201 tttgttataa atcagcgata acgcttcaac agcgatcggg taagcaatca gcttgccgtt
    10261 gtaacgtacg gcatcccagg taaacggata cagcttgtcc tggaacgctt tgtccggggt
    10321 gatttcagcc aacaggccag attgagcgta gccaccaaag cggtcgtgtg cccagaagat
    10381 aatgtcaggg ccatcgccag ttgccgcaac ctgagggaat ttctcttcca gtttatccgg
    10441 atgctcaacg gtgactttaa ttccggtatc tttctcgaat ttcttaccga cttcagcgag
    10501 accgttatag cctttatcgc cgttaatcca gattaccagt ttaccttctt cgattttggc
    10561 gagagccgag gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc
    10621 tgtttttatt ttcataatct atggtccttg ttggtgaagt gctcgtgaaa acacctaaac
    10681 ggactctagt ttctttatac ggcaacctct ttccatcctc cttgccccta cgccccaccg
    10741 ttgctttgtg tgatctctgt tacagaattg gcggtaatgt ggagatgcgc acataaaatc
    10801 gccacgattt ttgcaagcaa catcacgaaa ttccttacat gacctcggtt tagttcacag
    10861 aagccgcgtt ctcatcctcc cgcctcctcc cccataaaaa agccaggggg tggaggattt
    10921 aagccatctc ctgatgacgc atagtcagcc catcatgaat gttgctgtcg atgacaggtt
    10981 gttacaaagg gagaagggca tggcgagcgt acagctgcaa aatgtaacga aagcctgggg
    11041 tgaggtcgtg gtatcgaaag atatcaatct cgatatccat gaaggtgaat ttgtggtgtt
    11101 tgtcggaccg tctggctgcg gtaaatcgac tttactgcgc atgattgccg ggcttgagac
    11161 gatcaccagc ggcgacttgt tcatcggtga gaaacggatg aatgacactc cgccagcaga
    11221 acgcggcgtt ggtatggtgt ttcagtctta cgcgctctat ccccacctgt cagtagcaga
    11281 aaacatgtca tttggcctga aactggctgg cgcaaaaaaa gaggtgatta accaacgcgt
    11341 caaccaggtg gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct
    11401 ctccggtggt cagcgtcagc gcgtggcgat tggccgtacg ctggtggccg agccaagcgt
    11461 atttttgctc gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat
    11521 cgaaatctcc cgtctgcata aacgcctggg ccgcacaatg atttacgtca cccacgatca
    11581 ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgccggtc gcgtggcgca
    11641 ggttgggaaa ccgctggagc tgtaccacta tccggcagac cgttttgtcg ccggatttat
    11701 cggttcgcca aagatgaact tcctgccggt aaaagtgacc gccaccgcaa tcgatcaagt
    11761 gcaggtggag ctgccgatgc caaatcgtca gcaagtctgg ctgccagttg aaagccgtga
    11821 tgtccaggtt ggagccaata tgtcgctggg tattcgcccg gaacatctac tgccgagtga
    11881 tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac
    11941 ccaaatccat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt
    12001 ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc gttgccatct
    12061 gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg tttaagcacc
    12121 ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga
    12181 gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta
    12241 atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg tattggctgg
    12301 acaggtagcg gcggtgaaca acagtgtttc cagactaccg gtgctcaaag taaataccgt
    12361 cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag
    12421 ggcgataaga gcttctattt cgacactaac gtggcctatt ccgtcgcgca acagaatgac
    12481 tgggaagcta ccgacccggc cttccgtgaa gcaaacgtgc agggtaaaaa cctgatcgaa
    12541 tggctgccag gctccaccat ctgggcaggt aagcgcttct accaacgtca tgacgttcat
    12601 atgatcgact tctactactg ggatatttct ggtcctggtg ccggtctgga aaacatcgat
    12661 gttggcttcg gtaaactctc tctggcagca acccgctcct ctgaagcagg tggttcttcc
    12721 tctttcgcca gcaacaatat ttatgactat accaacgaaa ccgcgaacga cgttttcgat
    12781 gtgcgtttag cgcagatgga aatcaacccg ggcggcacat tagaactggg tgtcgactac
    12841 ggtcgtgcca acctgcgtga taactatcgt ctggttgatg gcgcatcgaa agacggctgg
    12901 ttattcactg ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag
    12961 tacgctactg actcgatgac ctcgcagggt aaaggtctgt cgcagggttc tggcgttgca
    13021 tttgataacg aaaaatttgc ctacaatatc aacaacaacg gtcacatgct gcgtatcctc
    13081 gaccacggtg cgatctccat gggcgacaac tgggacatga tgtacgtggg tatgtaccag
    13141 gatatcaact gggataacga caacggcacc aagtggtgga ccgtcggtat tcgcccgatg
    13201 tacaagtgga cgccaatcat gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc
    13261 cagcgcaccg gcgacaagaa caatcagtac aaaattaccc tcgcacaaca atggcaggct
    13321 ggcgacagca tctggtcacg cccggctatt cgtgtcttcg caacctacgc caagtgggat
    13381 gagaaatggg gttacgacta caacggcgat agcaaggtta acccgaacta cggcaaagcc
    13441 gttcctgctg atttcaacgg cggcagcttc ggtcgtggcg acagcgacga gtggaccttc
    13501 ggtgcccaga tggaaatctg gtggtaatag caaaacctgg gccggataag gtgcttgcgc
    13561 cgcatccggc atccaacgcc tgatgcgacg cttgcgcgtc ttatcaggcc tacaacggct
    13621 gtcaaatgta ggccggataa ggcgtttacg ccgcatccgg cataaaaaca ggttgtcatt
    13681 atctgaaagg ggcgcaagcc cctctgatta tcgggtttag cgcgctattg cctggctacc
    13741 gctgagctcc agattttgag gtgaaaacaa tgaaaatgaa taaaagtctc atcgccctct
    13801 gtttatcagc agggttgctg gcaagcgcgc ctggaatcag ccttgccgat gttaactacg
    13861 taccgcaaaa caccagcgac gcgccagcca ttccatctgc cgcgctgcaa caactcacct
    13921 ggacaccggt cgatcaatct aaaacccaga ccacccaact ggcgactggc ggccaacaac
    13981 tgaacgttgc tggcatcagt ggtccggttg ctgcgtacag cgtcccggca aacattggcg
    14041 aactgaccct gacgctgacc agcgaagtga acaaacaaac cagcgtattt gcgccgaacg
    14101 tgctgattct tgatcagaac atgaccccat cagccttctt tcccagcagt tatttcacct
    14161 accaggaacc aggcgtgatg agtgcagatc ggctggaagg cgttatgcgc ctgacaccgg
    14221 cgttggggca gcaaaaactt tatgttctgg tctttaccac ggaaaaagat ctccagcaga
    14281 cgacccaact gctcgacccg gctaaagcct atgccaaagg cgttggtaac tcgatcccgg
    14341 atattcccga tccggttgct cgtcatacca ccgatggctt actgaaactg aaagtgaaaa
    14401 cgaactccag ctccagcgtg ttggtcgggc ctttatttgg ttcttccgct ccagctccgg
    14461 ttacggtagg caacacggcg gcaccagctg tggctgcacc cgctccggtg ccggtgaaga
    14521 aaagcgagcc gatgctcaac gacacggaaa gttattttaa taccgcgatc aaaaacgctg
    14581 tcgcgaaagg tgatgttgat aaggcgttaa aactgcttga tgaagctgaa cgcctgggat
    14641 cgacatctgc ccgttccacc tttatcagca gtgtaaaagg caaggggtaa ttacgcccca
    14701 cagtgctgat tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta tgcttcattc
    14761 ctgggatatg agcgattttt tatagtaact cacttcttct tcactaagaa tatccattat
    14821 ctcaatgcct tatcagagat tcttttcctt tcgccggtag tgtctggaca ttcaggctac
    14881 ttttccaggt tatttcattt ctgtaatgca gaggttttat gttaggtcat atctcaaagt
    14941 ttgacggcaa taactctttg ataaaacatg gtgtggtgca aggaaataat atagtagatt
    15001 ttgatttact acgtaatttt aatggggtgc cagggttaaa tc
//
LOCUS       DAZGBC010000120        14916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGBC010000120 DAZGBC010000000
VERSION     DAZGBC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.121"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..631
                     /locus_tag="ACHK1J_003547"
     CDS             <1..631
                     /locus_tag="ACHK1J_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFO7912772.1"
                     /translation="NAVRFMIITGVRPINVHNLRWDYVHEEAGEIVYPEGVIGMRGAM
                     KTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRDYVFLQPRDPMQPFSKRSLDKLVKT
                     YSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSNVIALMKERGYSRSDRREISLLCLH
                     HSSKSDDPMAEHYDFSDEILQEEIALKREAFEAHERSILAQVALLRRRG"
     gene            complement(628..828)
                     /locus_tag="ACHK1J_003548"
     CDS             complement(628..828)
                     /locus_tag="ACHK1J_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7912773.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(825..2219)
                     /locus_tag="ACHK1J_003549"
     CDS             complement(825..2219)
                     /locus_tag="ACHK1J_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO7912774.1"
                     /translation="MGQIFTPRPYQDLIINHEIGISRCNIWAGMGMGKTVATLTTLED
                     LFMAGSETQPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVQ
                     RWINLTGTPAPNGLVDLWGQLWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQAEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(2264..3043)
                     /locus_tag="ACHK1J_003550"
     CDS             complement(2264..3043)
                     /locus_tag="ACHK1J_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7912775.1"
                     /translation="MSERTLTLAGTGKQPMMSSREIAEVVNKNHADVCRDIRAMLAKL
                     YGGDERDYIRKADLLYLTNQCVNCLQYNTDNPNAWEYQLDYEHSECLVTGYDAVRRMA
                     MIKHWQKLKKEVEPPQLAITHPAPVIQVDSRIVQLARVIAEATASATMKAMSEVIALP
                     TYQAESAAPANLSHVEGEYVPVSKAAWKTGLSDSTCRKLIGFARIPVRSDTGVRGLLV
                     NLPAIEQAAQKLLNESTPPKGNRKRWTHPQFGNFTYYTDLI"
     gene            complement(3104..3280)
                     /locus_tag="ACHK1J_003551"
     CDS             complement(3104..3280)
                     /locus_tag="ACHK1J_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912776.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            3393..3722
                     /locus_tag="ACHK1J_003552"
     CDS             3393..3722
                     /locus_tag="ACHK1J_003552"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO7912777.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQVEWVYENKYELIEVMEKLKK
                     LLNPENE"
     gene            complement(3691..3867)
                     /locus_tag="ACHK1J_003553"
     CDS             complement(3691..3867)
                     /locus_tag="ACHK1J_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO7912778.1"
                     /translation="MSKRDDPQLRVRIPQDLKDTLEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(3864..4133)
                     /locus_tag="ACHK1J_003554"
     CDS             complement(3864..4133)
                     /locus_tag="ACHK1J_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO7912779.1"
                     /translation="MAYERENLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKAPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            complement(4123..4422)
                     /locus_tag="ACHK1J_003555"
     CDS             complement(4123..4422)
                     /locus_tag="ACHK1J_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO7912780.1"
                     /translation="MIVYVAGPMSGYEQFNRPAFHSAAKRLTDKGYVVLNPATLPDGL
                     TQAQYMDICLAMLRCADTIYMLKEWEYSAGARAENALAEKLELNVLFEEWDSDGL"
     gene            complement(4419..4634)
                     /locus_tag="ACHK1J_003556"
     CDS             complement(4419..4634)
                     /locus_tag="ACHK1J_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7912781.1"
                     /translation="MSFKYRDSPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVEE"
     gene            complement(4687..5454)
                     /locus_tag="ACHK1J_003557"
     CDS             complement(4687..5454)
                     /locus_tag="ACHK1J_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO7912782.1"
                     /translation="MTSQIIPVFNGTIANETTLLCNARDLHAFLGVGKMFAHWIKDRL
                     GEYGFVENQDFVIACQNWQAKGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYFI
                     ECERKLHQQSSTILAPHRECLPKMVYHHSSKYNPYRAYAWDGEKIVYVGCYPTVDEAV
                     AAQERFYQNGSTKRIQKVQTAINDAEKEMFINNLRAVCHNFRRINEIWRAQLMPALEK
                     MDSKLVYQLHDRFSDSMCILPTIEDRIGRYIPPTLPR"
     gene            complement(5694..7757)
                     /locus_tag="ACHK1J_003558"
     CDS             complement(5694..7757)
                     /locus_tag="ACHK1J_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO7912783.1"
                     /translation="MSNILWGDLETFCEIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIAAPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDMQLARAAIEAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPHLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYNESVKRKSTFGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEQGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITEAPDSPDYSAEHLSQLLATVPDWATNIPLNAGGFESY
                     RYRKD"
     gene            7784..8383
                     /locus_tag="ACHK1J_003559"
     CDS             7784..8383
                     /locus_tag="ACHK1J_003559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912784.1"
                     /translation="MDTKDIISLVGVIISAITAPVGVYKLFKDASWFLPKTTKFSHIL
                     KNYPEHIEPSDVEFMKAEIKREVKKSILGISSQKLRTLVIYVRTYSELKMPFWQWGYL
                     APHIQCKYDRFFIRYKGKYKRCRWCSKVVSIFYLADGLIFFCWSLNYGAVFIGMGAVL
                     MLLCIWMAFMFWFLFPGRGVIKKYNSQLLKIDASKYQAK"
     gene            complement(8498..9262)
                     /locus_tag="ACHK1J_003560"
     CDS             complement(8498..9262)
                     /locus_tag="ACHK1J_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO7912785.1"
                     /translation="MSTQIVFKSHVLETIEHNGKTWFTAATLATALEYSDARSVTNIY
                     TRNTDEFTLGMSEVIKMVTSGNLQASRRIFSLRGAHLVAMFATTPVAKEFRRWVLDLI
                     EKETAVPQSSTILAPHRECLPKMVYHHRSKYNPYRAYAWDGEKSVYVGCYPTVGEAVA
                     AQERFYRNGSTKRIQKAQPAVSDAEKEMFINNLRAVCHNFRRINEIWRAQLMPALEKM
                     DSKLVYQLHDRFSDSMCILPTIEDRIGRYIPPTLPR"
     gene            complement(9644..10192)
                     /locus_tag="ACHK1J_003561"
     CDS             complement(9644..10192)
                     /locus_tag="ACHK1J_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO7912786.1"
                     /translation="MKIKLNNVRLAFPALFEAKTVNGEGDPRFSAVFLISPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(10208..11509)
                     /locus_tag="ACHK1J_003562"
     CDS             complement(10208..11509)
                     /locus_tag="ACHK1J_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO7912787.1"
                     /translation="MPDVHARLSPSSAHRWLRCAGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGKRAQEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGNPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDLKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLAADLI"
     gene            complement(11502..12020)
                     /locus_tag="ACHK1J_003563"
     CDS             complement(11502..12020)
                     /locus_tag="ACHK1J_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFO7912788.1"
                     /translation="MPEAWKDISGFEGIYQISDAGNLRSLDRTVLNKGSGCTYPIKGK
                     SLKPRYDADGYLITDLWKAGKKVTVKIHRLVAAAFIPGSAPEVDHINGRRDDNRAVNL
                     RWASLSQNRANSHTRKNKSGVVGVRFSEGKKNPWQAYGRLNGKFKSLGHFPTKQLVAA
                     ARRKHIQEVMNA"
     gene            complement(12023..12925)
                     /locus_tag="ACHK1J_003564"
     CDS             complement(12023..12925)
                     /locus_tag="ACHK1J_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912789.1"
                     /translation="MSLETSLELNNQLLEQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(12922..13071)
                     /locus_tag="ACHK1J_003565"
     CDS             complement(12922..13071)
                     /locus_tag="ACHK1J_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912790.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(13173..13466)
                     /locus_tag="ACHK1J_003566"
     CDS             complement(13173..13466)
                     /locus_tag="ACHK1J_003566"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO7912791.1"
                     /translation="MAGKPAGKVKADGYVTICLDGKNQYVHRLVWEYHFGDIPAGMEI
                     DHINHNRADNRIENLRLVTRAENAKNLAQRSPTKRNGGQLERIKKALAGTNHR"
     gene            complement(13654..13860)
                     /locus_tag="ACHK1J_003567"
     CDS             complement(13654..13860)
                     /locus_tag="ACHK1J_003567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912792.1"
                     /translation="MNTYELTTKYAPAFEVVENGPDATDYVVATVKGENPARLRAAAY
                     QERNGYLGYGYTIRPLTNSSESSM"
     gene            complement(14245..14874)
                     /locus_tag="ACHK1J_003568"
     CDS             complement(14245..14874)
                     /locus_tag="ACHK1J_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7912793.1"
                     /translation="MNLANRAKKRRTELNLTQVEVAKRAGISQQSIEAIENGKTLKPR
                     NLLALASALECDPKWLLLGGDVVTDFNYGARRVPVLSYVQAGAFTSAELLREEGDFEY
                     ILTTAELSESSFALRIRGDSMEPEFKEGDIVIIDTDVYPTPGEFVAACNGSHEATFKK
                     YRPVGIGAKGEEDFELVPLNSDYPICRSWEKPIKIIGTMVEHRIFRRKR"
BASE COUNT         3396 a         3623 c         3766 g         4131 t
ORIGIN      
        1 gaatgctgtt cgcttcatga tcatcactgg tgttcgtccg attaatgtcc ataacctgcg
       61 ctgggactac gttcacgagg aggctggtga aattgtttat ccggaagggg ttatcggcat
      121 gcgaggggct atgaaaacac aaaaggcttt ccgcctgccg ataacgcctg agatccggcg
      181 gattatcgac gagcagaaag cctggcgtga ttcagttcct gagtgcaaca gggattatgt
      241 atttttgcag ccacgtgatc caatgcagcc attttcaaaa cgatcactgg ataagctggt
      301 gaaaacatac agcccggacg gggctgtaaa aggaataaaa catgatggga ctgttaaagg
      361 gaaagacggt gcatttaata cgatgtgccg taaattcctt aagagcaatg ttattgcctt
      421 gatgaaggaa agaggctatt cccgctcaga ccgaagggaa atcagcctcc tttgccttca
      481 ccactccagc aagtcagatg acccgatggc agaacattac gacttttctg atgagatttt
      541 acaggaagag attgcgttga agcgcgaagc tttcgaggct cacgagcgga gcatacttgc
      601 gcaggtggca ttgctacggc ggcgaggtta atactggctg cgacattttt gaataaaagc
      661 gtcgacattt cggcgctcat aacgaactac ttttgcactg aaacgaattg gtgccaggat
      721 agcccgatga cgatgcttaa tattccactc acatagcgtt ttctgtgtaa tacctaactt
      781 ttggcatact tcatccgggg tgagtaaatc gtcgggtttc tcgctcatgc tatacctctc
      841 tttttcatgg catcgagcag gatgtcctgc actgttcgtt ttgagttgcg ccgttccatc
      901 accatttcgt ccatagtgtc ggcggcgata atgtggtgaa taaataccgg acgattgtgt
      961 ccggcctgta tctgcctggt ggggccgata cgttcaataa tttgctgata ttgctccagg
     1021 tcccaccagt gtgagaaaaa taccagtata tttccgccgt cctgcatgtt caggccgtgg
     1081 cccgcgctgg ctgggtgtgc aaagagaaca ggaatctttc cggaattcca gtcgcgcagt
     1141 gtctgtggat cctggtcgag gtgacgaccg cgagggaatg ctttaagcaa gcgttcaaga
     1201 tcgtgtttcc agtgataagc aaccagcaca ggtgcgccag ctgcttcggt cagtatgctg
     1261 tccagcgcct gtagtttggt gtcatgcagt tctgaccaac ttccggtgtc gtctgtgtat
     1321 actgcgccac tggcgatttg aagacacttc agtgtctttg ccgcggcgtt cggtgcttcg
     1381 atgccttcgc cattcagctc gaggaacatt tccttttcca tttcacgata ctgctgacgg
     1441 gccttcggcg gcatatccac gcggattacg ttatggatgg ggtctttgat atcgaaccag
     1501 tcggccgcat ccagcgagag ggtcacatcg gctaacgctc gctgtatttc agcctgtgag
     1561 tgagcaaacg gctccagttt ggtccagctc tgccccggaa actgtatcga gttgaaccag
     1621 cgtgaggtaa acgcgccgta agtgcgcccg agacgttgcc cctggtccac aaaccacaat
     1681 tgtccccaca aatctaccag gccgttcggt gctggcgtac cggtgagatt tatccagcgc
     1741 tggacatact tatgcgccac ttttcccagc gccgccgcgc gcttaccacc acctcgcagc
     1801 cggaaggatt ttagccgggt gctttcatct ggaatgacag taccgaacgg ccatcgttct
     1861 cccaattcct caaccagcca gacaaggtta tcgtagttga tggtgaacac gctcgcgttg
     1921 ctgttcgcca gcgctgcaga gcgcgctttg gcgttaccaa caatcggctg cacctcgata
     1981 ttgcgcagat gcccccattt cagcgcttca tccggccatg tgcttgctgc aacgcgtagc
     2041 ggcgcgagga ccagcgcggg ctgtgtctct gagcccgcca tgaaaagatc ttccagcgtg
     2101 gtgagtgtcg ctacggtttt acccatcccc atccctgccc agatgttgca gcgggaaata
     2161 cctatttcgt ggttgataat tagatcttgg taggggcgag gcgtgaaaat ttgtcccata
     2221 gtattttagc cagtagggcg cggggtaatc ccgcgcaata atgtcagatt agatcggtgt
     2281 agtaggtgaa gttgccaaac tgcggatgtg tccagcgctt gcggttacct ttcggcggtg
     2341 tcgattcgtt taacagcttt tgagctgctt gttcgatcgc cggaaggtta acgagcaatc
     2401 cccgaacacc ggtgtctgaa cgtaccggta tcctggcgaa gccgatcaat ttccggcatg
     2461 ttgaatcaga taaacctgtt ttccatgctg ctttgctgac ggggacatat tcaccttcaa
     2521 catgagataa attcgctggt gcggccgatt ctgcttgata tgtcggaagg gcaatcacct
     2581 ccgacatcgc tttcatggtt gctgatgcgg ttgcctccgc gatgacgcga gcaagctgga
     2641 caattctact atccacctga attacaggtg cggggtgagt gatagctaac tgggggggct
     2701 cgacttcttt tttcagtttt tgccagtgct taatcatcgc catacgacga acggcatcgt
     2761 aacctgttac aaggcattcg gagtgttcat agtcaagttg gtactcccat gcgtttggat
     2821 tatcggtatt gtactgcagg caattaacac actgattcgt aaggtaaagc aaatcggctt
     2881 tacggatata atcgcgctca tcaccgccgt aaagctttgc cagcattgca cgaatatcac
     2941 ggcaaacatc ggcatggttt ttatttacca cttctgctat ctcgcgactg ctcatcatcg
     3001 gttgcttacc agtgcctgct agtgttaaag ttctttcgga cattgaagtg ttctccattc
     3061 agtaaaagat gcgctgatag ttgcagctac cagcgcgttt tgtttattca tcccacggct
     3121 tgtttgcagc ctcccttgcg gcctgctgtg caaaataaat caattcaaaa gctaactgct
     3181 ctcctttttc cagcgaactg agaagtcttg ctgtttccag catgtccgcg atatacccga
     3241 aaatatctgc ctgatgtaac tgcgtgatct gaatagccat agcgttaacc ttgttttgta
     3301 ctcattgtga ggactttatt agtactcaca atgaggcttg aagtcaacat gtttgtgagg
     3361 catgttgttc tcacactttc aagggatcat atatgagcaa aagagacgac ccgcagttga
     3421 gggtacgcat accagaattg ctaaaagagg ctttggagaa gaaagcacgg gctaataaga
     3481 gaacactgac ctctgagatc gttagccggt tggaatctac tattcttcag gacaaattac
     3541 ttcatacatc tagggggttt gaggaagctg ctgatgagat tctcatactc agggatcttc
     3601 tcgagaaact taagacaacg taccatcgag agtatcaggt cgaatgggtt tatgaaaaca
     3661 aatacgagct tattgaagtg atggaaaaac ttaagaagtt gctaaatcct gagaatgaat
     3721 agtggattca tcctctaaac ttttacgtaa ccgccgggtg atttcggcgg ttagtgttct
     3781 gtcgttttct ctggaaattt tttccagtgt gtctttcaag tcttgcggga tgcgtacccg
     3841 caattgtggg tcatcacgtt tactcacaat attccctcca gatttttgct atccagcacc
     3901 accacggtaa agcccagctt tcgcagacgt tcatgctcgc gcaactggtc aggccgtggt
     3961 ggcttgcctg gcgctttgca ttcgacaaag acgatgcgac cgccgggtag caggacaatg
     4021 cgatccggta ccgagcggcg accgggtgac acgaacttaa aggcgacccc gccagccttt
     4081 ttcacttcgg cgacgagatg cttctctatt aggttttcac gttcataggc catctgaatc
     4141 ccactcttca aaaagcacat tcaattccag tttctctgcc aatgcgtttt ctgcacgtgc
     4201 gccggcagag tactcccact ctttgagcat ataaatcgta tcggcacagc gaagcattgc
     4261 gaggcagatg tccatatact gagcctgtgt caaaccatcg ggtaaagttg cggggttaag
     4321 cacaacatat cccttgtccg ttagcctctt tgctgcggaa tgaaaagccg ggcggttaaa
     4381 ttgctcgtaa ccgctcatcg gcccggcaac gtaaacgatc attcttcgac cgccttacgc
     4441 ttttcgcgca tgttctgcat cagacaaaaa tcagaccggc gttcgctcca gtcctgattc
     4501 agttcgttac gtgactggcg gttagccttg gcccagacct tcgctgcccg gtcatattcg
     4561 ccggactgct caaggcgcaa ggcctccctc gcagcccggt aataaagcgg actgtcccgg
     4621 tatttaaatg acatatcttt cgactcctgt aaaaagcccc tggctgagca ggggcgaact
     4681 gaaggattaa cgaggtaaag tgggtgggat atatctgccg atgcggtctt ctatggtcgg
     4741 cagaatgcac atgctgtcgc tgaagcggtc atgcaactgg taaaccagtt tcgaatccat
     4801 tttttcgaga gcaggcatta actgcgcccg ccagatttca ttgatgcgcc ggaagttatg
     4861 acagacggcg cggagattgt tgatgaacat ttccttctct gcgtcattga ttgctgtctg
     4921 cactttctga atgcgtttcg tgctgccgtt ctggtagaag cgctcctgcg cagcgacagc
     4981 ctcatccaca gtcggatagc acccgacgta aacgattttc tctccgtccc atgcataagc
     5041 cctgtacggg ttgtatttgc tactgtggtg gtaaaccatc ttcggcagac actccctgtg
     5101 cggcgctaat attgtggagg actgctggtg cagtttgcgt tcgcactcga tgaagtatcg
     5161 gcggatctgg cggccttttt cgttacgttc gaccattgcc agttctttag cggtgtcgag
     5221 ggtgaggtgg tagtccttgc ggttgtgacc gcctctacct tttgcttgcc aattttggca
     5281 agcaatcaca aaatcttgat tttcaacgaa tccatattca cccagcctgt ccttgatcca
     5341 gtgagcaaac attttaccca caccaagaaa agcgtgcaga tcacgggcat tacagagtag
     5401 ggtggtttcg ttggcaatag tgccgttgaa tacggggatg atttgactag tcatttttat
     5461 gtcctttgca atattttgaa ttcacccctt ttgagagggt ggtcaggcgc tcaaaaccgt
     5521 gcaaagtcgg cgggcgtatt tcccttacgg gtattgtatt agccgcacgc ccgaccattg
     5581 aattctcaat atcgtggaca taaaaaatcc gcatgactgt cgggtgcggt ttccgctttg
     5641 caaggtgttt tgagcaccat gaagcggaat atacctattg gtcatcgcgg atgtcaatct
     5701 ttccggtatc tgtaagattc gaatcctccg gcgtttagtg gaatgttcgt ggcccagtca
     5761 ggaacggttg ccagtaactg gctgagatgt tctgctgagt aatcgggtga gtcaggtgct
     5821 tcggtgatca cctcgtcgtg taccgtcagc acaatgctgt aaccggcatc ttcgataagc
     5881 ggcatgtttc cggccagaac gtcgcgggcg gccgcctggg tgacgttctc taccagcttt
     5941 ccgccgtagg ttttgagtcg ttgccattta cgcgaataag agttaacacc catgtaggtg
     6001 atattccctt gttcgataac cggagacggg tagcatacag cgcgtccgga tggtagctgt
     6061 atgcgcagcc acgcgccatc gcggcggatt ttaagataac cgcaatacaa tgttttttgc
     6121 ggtgtggcga ttgcagtgcg gacggttcgc tccagttcgt accagaaatc gcaggtcgcc
     6181 gggtgcgccc tgcgccacag gcgcttgagc gagtcacatg cgatgaatac acgctcggac
     6241 aggccaaagg tcgacttacg tttaaccgat tcgttgtacc agcttttcgc ctcgcggata
     6301 acatcgcggg gaatgtttgg cagtgcggcg ttcgccagct cgtcgagatc gagaccgtaa
     6361 accagagcaa aagtgatgaa agccgataca ccacctccat aacccagacc gagttccatg
     6421 actttaccga tctgacgcat gtgtttatca acatcatctg gtgcaatatc gaaagctttt
     6481 gcatacgcca gtttatataa gtccggaccc gttccggcgt catactctct gaatgcattc
     6541 agtttccatt cttctcccgc cagccatgcc agcatgcggc cttcaatgtt cgacaagtca
     6601 cttaccacca gttttttgcc tgttggcgcg ataatgcagc cacgtaacgc tgaactggtt
     6661 agttccatga tattgtcaaa cagcaggtct gcacatccgg ctttcagtgc ttcgatgcct
     6721 tcgtctattt gttcctgttt tagtgaaggg cggggaaggt tctggggctg gaatagccgt
     6781 ccggcccaac gaccggtacg tgacgccccg cagaactgta gcgtaccgcg taagcgcccg
     6841 tcgtggctta cgcctttcat cagtgccttg tatttactgg tgctggtagt actggcttgc
     6901 aggcggatag ccagcagttc tttcacggca gatggtaaat gggggtcggc aatacgacgt
     6961 tccagagtac tgcgttgcat gtctggtagc tccacaccgt aggattcaac aatgtgctta
     7021 atcaacgcgt ctcgttgtgt ggctgcctgc acttcgccat cagtcatttc ctgtgtacgc
     7081 tttgccaggc gcttttgctc ctggtctacc gcttcgatcg cagcgcgcgc gagttgcatg
     7141 tccatgcaga cgccccggtc gttgatctgc tgatcacgat gccagagcgc cagctctgtc
     7201 ccctgataat tccacttcgg cagacgttta tagacttcgc gcattgcctc gatatccagt
     7261 ccggcgtaag caacaaagcg ccgccattct tccgggtggg ttttgctggt ggcacggcgc
     7321 agtttgctgt ttttcgggcg gggcttacag aacagctgga tcagcgcttt accttctttg
     7381 tccttcgctt tgtcttgcgg gacgcctagt acttcgcaga gttcccccag agaccccggg
     7441 agaccgtgcg ccagcgcctg caccattgtg tcgcgccaac gttcgactgg cggtgccagg
     7501 cgcggcattg cataacgcag aacggtgcgg tcgaagtgag agttatggaa ataaagcagg
     7561 gtttcagggg ctgcgattgc ttcgtataag ccgtgtggaa taccaccacc ggcagtgata
     7621 tcccacacgt ttactggccc gtcgttgata gcccatgcga aaagcatcac ttcaacgcct
     7681 tcggcatacg catgggtgcc attattgata ggtatttcac agaaagtttc caggtcgccc
     7741 catagtatat tggacattgt atttactccg aagaattagc tacatggata cgaaagatat
     7801 tatctcactt gttggagtca taatttcagc cataactgca ccagtcggtg tttataagtt
     7861 atttaaagat gctagttggt ttttaccgaa aacaacaaaa ttctcgcata ttctgaagaa
     7921 ttatccggag catattgagc cttctgatgt agagtttatg aaggcagaaa tcaaaagaga
     7981 agttaaaaaa agtatattag gtataagtag ccagaaatta agaacgttag ttatttatgt
     8041 cagaacgtat tctgaattga aaatgccgtt ttggcagtgg ggttatttag caccccatat
     8101 tcagtgtaaa tacgatagat tctttatacg ctacaaaggt aaatacaaac gatgtcgctg
     8161 gtgttctaaa gttgtttcta tcttttattt ggcagatggc ttgatatttt tctgttggtc
     8221 gttaaattat ggcgctgttt ttataggaat gggcgctgtt ttaatgttgc tgtgtatatg
     8281 gatggctttt atgttttggt ttctttttcc gggacgaggc gttataaaga agtataattc
     8341 ccaattattg aaaatagatg caagtaagta tcaggccaaa tgatattaca ggtctgatac
     8401 tttgcttttt taaatcagtg ttttcagaca aaacgaagtc ctccgtaaat taaacggtga
     8461 taaaaagccc ctggctgagc aggggcgaac tgaaggatta acgaggtaaa gtgggtggga
     8521 tatatctgcc gatgcggtct tctatggtcg gcagaatgca catgctgtcg ctgaagcggt
     8581 catgcaactg gtaaaccagt ttcgaatcca ttttttcgag agcaggcatt aactgcgccc
     8641 gccagatttc gttgatgcgc cggaagttat gacagacggc gcggagattg ttgatgaaca
     8701 tttccttctc tgcgtcactg actgctggct gcgctttctg aatgcgtttc gtgctgccat
     8761 tccggtagaa gcgttcctga gctgcaacag cctcacccac ggttggatag cacccgacgt
     8821 aaacgctttt ctctccgtcc catgcataag ccctgtacgg attgtatttg ctccggtgat
     8881 ggtaaaccat tttcggcaga cactccctgt gcggcgccag tatcgtggag gattgcggaa
     8941 cagcggtttc tttctcgata aggtcaagca cccaacggcg gaattctttt gctaccggag
     9001 ttgttgcgaa catagctacc aggtgggcac cgcggaggga gaaaatgcgg cgcgaggcct
     9061 gtaaattccc cgaggtaacc attttgatta cctcggacat gccgagagtg aactcatccg
     9121 tgttgcgggt gtaaatgtta gttacgctgc gagcatccga atattccagc gcggtagcta
     9181 gggtagccgc agtaaaccaa gttttgccgt tatgttcgat cgtttcgaga acgtgggatt
     9241 tgaatacgat ttgggtactc atgacgattt tcctcttaac tgagttaatc gccaccatca
     9301 ggtgctaatc atcgtggtgg cgaactgtgc ggggttagca ctaccgggtt aagaggaacc
     9361 ggcgcggatt tctccgcccc cacacagccc gccataatgc gaacgtggca aagcttgcga
     9421 caataaaaag acgctggcgc gtctggtgtc gcctcttaaa cttccggggt gctaatcccg
     9481 atctccattt gggagattcg gtgagcataa accggaaggg ggcgatgagt caatatcggg
     9541 gtagcagggc gactgggtgc taaccagaac aatacaggta gctccgggca tattcccctt
     9601 tgcaaagaca cccggcgttt aaccgggcgt ctgtgaagca ggattaaatc agtgcttcag
     9661 catcagcacc ttcgctgata tcgtcgaaat cgtcagcgct tgctactccg ccgccagcga
     9721 atgcatcgcc gtctcgcagg aactggactc cgccgagtga ggcattaatg cgtttaccga
     9781 aattattgtc ctgtgcccag atatcgataa cggcgtttac atagcaccct gcatagggac
     9841 gtccatcagc ctgaattagt ggcgaacgat cgcgatcaag aacggccggg cgcgctttgt
     9901 tagcagcatt caggaagaaa ttaccgggga agccttcata ctctgctttt tcatcaccat
     9961 catgcaggca cagattgagt tttttctcca gctggttata aatggactcc cacttctccc
    10021 cccatttttc cttcgctacc tgcttcatag ctttacggat ttcttccagt tgtgggtgtt
    10081 tgggagatat taaaaatact gcggagaaac gcggatcgcc ttcgccgttt acagttttag
    10141 cttcaaacag agcaggaaaa gctaatctca cgttatttaa tttaatcttc atgaaagtct
    10201 cctttaatca gatgaggtct gcggcgagcg tatcgtcgga tacgtcgtcg aaatcattta
    10261 cagggttgat attgagtgcg gggcgtgggt ccgactcggg aacgacggtt ggtttaccat
    10321 cagctcgtgt tatcagtgcc tcgacttttg accaacggcg cggactggcc tttttgataa
    10381 gtttttcggc tttggttggg ctaatcagct taagatcgaa aacctcctcg gttttataac
    10441 ggaactggtc tttcagcagc gcgcgggcgg cttcttcatc actccaggcc cggttacctt
    10501 gttttcctgt taccagttta aaccccggta ccggatttcc ggcattgagt tcattatgaa
    10561 cccggtcccg tactgccttt agccaggatt caataaagtc ggcctgacta tagatctccg
    10621 caagctgctc aatggttaac agaggtacac gtgcgctggc attggtgatg atttcgctga
    10681 caggctttgt cagatcttca aaatcgctgg ccgctgtttg taaatgctgc attttctggg
    10741 cagtgcaaat agcttttgct ttacagaagc ggcactgttt ttctccaggt atgaagtttt
    10801 ccagcggtag tgtctcaatg ccttcgcatt cagcaatatt gagaacaagg atcgcactgg
    10861 ttgcggcctc ctgtgctcgt ttaccgaaag actgaagctc ctgtacggtt aaagaccatt
    10921 ctgaaacgtg gttgagcctt ggctggtgaa taaataatct tacagtctca aagtcataca
    10981 gcatgctgaa ttgttcgaga gcacccagag catacagttg tagttgctca ttttgttctg
    11041 catcaatgcg gacgcctttg ccatatttca ggtcgtggat ttgtaattcg ttaccagcga
    11101 tgattatgcc gtcggcagtt ccgaaagatt cttccacacc cgttatatgt gagaaatcaa
    11161 cacgttgttc aaccaatagt tcattattct gtgcaagagt ccagaccgta tcaacatacc
    11221 ggccaacggc ttcgaccatt tcatcatcca cctgtgggcc agatgtatca tcaggatttt
    11281 cgcgaagggg gtatgagccg agaaacatag aaacattgca tccggcgtag tgttccgggt
    11341 ggctttgcct gtttcgtaga actttttcag caagcgcgtg cgctgcagtg ccctcgattg
    11401 caaaagttgt ttctttatcc ggttgtgtgg cctccagcgc cagactaccc gcacacctta
    11461 gccaacgatg tgcagatgat ggtgataatc gtgcatgaac atcaggcatt catgacctcc
    11521 tgaatgtgtt ttcgccgcgc agccgctacg agttgtttgg tggggaaatg accgagagat
    11581 ttgaatttgc cgtttaatcg tccgtaggcc tgccacgggt ttttcttacc ttcgctgaac
    11641 cgaacgccaa ccactccaga tttatttttt ctggtgtgag agttagccct gttttgtgaa
    11701 agagaggccc agcgaagatt taccgcccgg ttgtcatctc gacgaccgtt aatgtggtca
    11761 acttcagggg cagatccggg aataaatgca gcggcaacga gacggtgtat tttgacggtt
    11821 acctttttac ccgctttcca taagtcggtg atgagatatc cgtctgcgtc atagcgtggt
    11881 tttagtgact tacctttaat gggataggtg cagccagaac ctttgtttaa tacagttctg
    11941 tcgaggcttc tcaggttgcc agcgtctgaa atctggtaaa ttccttcaaa gcctgaaata
    12001 tccttccagg cttccggcat gattaaccct ccagtgcttt ttcagccagg gtgattactt
    12061 cagcgagatt ttcatctgtt acttcaccaa gtttcctggc tccctgtttt tccagaattg
    12121 caatagcttc tgcccggtaa cccccttttg ctaactggag gatcaaccct tcagcttgtt
    12181 tgcgtagtgc cgcgaaatca attgtatggt catctttggc gtcattattc tggctggaat
    12241 ttgctgcgtc tctgcgtgca aattcttcct gcagctgaag gtactcaaca cggttgatct
    12301 cgatatggcc ttttttcagc atctcgttca acttgcgtaa ggtgtggagt tcactggctg
    12361 ctgtgccgga tacatttttg acgtaaaacg gccccgtgcg ttctccatct ttgttactgg
    12421 cctttttcgg cttaacttca tcacgcccat ctgcaggtgc atcaagtagc tgctcggcaa
    12481 aagcacgtcg ctcgccgatg gttggcaggt cgtcccagaa cttaagaatg ttacgggaca
    12541 ggtccaggag agcaggttta agcagcgccc tggctcgttt gacgccctgt aatgcgctgt
    12601 cgagagcatc aatctgaact actcgtttat cgccttcagc atcacggtag gcaacagcac
    12661 gttgcagcat gtcttctgtg ataggggtgg ctaccgggta gaaaccagcc agtgcgataa
    12721 cgtcgctgaa ctccagatca tccagtgtca ttgccgctga catgttttca gcttcagttg
    12781 ctgtatcccg acattcctgc actcgtgaaa tcgtgtcagg atgcataaca atacctgatg
    12841 ccattgtgcg gataagacgt tcaagcagcg cattatgttg ttccagaagt tgattattaa
    12901 gttcgagact ggtttctaaa ctcatactgt ggtcctcgct acaaggagaa tgaaagtgat
    12961 gatcagaccg agcgcagtgg caacggccag accggtcatc aaatcgaagt ttttacggcg
    13021 ataacggaga acatcgcgcc ccgtcagtcg atagaggtgt tcaggtttca tcggttgtgc
    13081 tcctaaaacg cccatgcgct ttttcggctc ggatacgtgc ggcaatggcg ctatcaagtt
    13141 cggggaaata cccgaggtgg agtggtttac cgttaacggt gatttgtgcc cgccaacgct
    13201 ttcttgattc tttccagttg acccccattg cgttttgtgg ggctacgctg tgcaaggttt
    13261 ttggcgttct cagcgcgtgt gaccagacgt aagttttcaa tgcggttatc cgcccggtta
    13321 tgattgatat ggtcaatttc catacctgcg gggatatcgc cgaagtggta ttcccagacg
    13381 aggcgatgaa cgtactgatt ttttccatca aggcagatgg tgacataacc gtctgctttt
    13441 acttttcccg cgggcttgcc tgccatgcgt gaattagtgg atttccatgc gtgggggctt
    13501 ttgaaatgcg atgcgggtcg gtatttccat ataagctggc cgttctggta ttcaaaagcc
    13561 tccctgattc tctttttcaa cgtaggagtc ctcgtgaagg taggaatgaa aaaaagcccg
    13621 tcatgggagg cgggcaaaga ctacacatag caattacatg gatgattcag aagaattagt
    13681 tagcggacgg attgtgtagc cataaccgag atagccgttg cgttcctggt aggccgccgc
    13741 tcttagtcgg gctgggtttt cgcctttgac ggttgcaacg acgtaatcag tcgcgtcagg
    13801 tccgttttct accacttcaa aagccggagc gtattttgtt gtgagctcat aggtattcat
    13861 ttcggtaccc cttcagtgga ttagtaaaag gcccgaagcc tttgattaat tcactgcacg
    13921 ccccatcatc ggggcgtttc aatttacgtg acttatcagc tcgtcgcggt gttgtcctct
    13981 acgcttaccg tacgcatacg gactcggcgc ttacctcgat cccatcgggt gccatttcat
    14041 tttgccagga gtactgcggc ttacctgtca cgcggttcag tttgttaaag agcgattact
    14101 tagtgcggtt aaaaatacaa gcaaacctgt aagtgcgtca acaggtattc tggtaataaa
    14161 atgcattatt aacctaatta cttgttttta caggtaataa aaaaagaggg cctaagccct
    14221 ctttgtttag tagtcaggat gcgattatct ttttctgcgg aatatgcgat gttctaccat
    14281 tgtccctatg atcttaattg gtttttccca ggagcgacaa atagggtaat cactattcaa
    14341 tggaacaagt tcaaagtctt cctcaccctt agcgccaatc cctaccgggc ggtatttttt
    14401 gaacgttgct tcgtggctac cgttacatgc cgcaacgaat tcaccaggag tagggtatac
    14461 gtcagtgtca attataacga tgtccccctc tttaaattca ggttccattg aatccccacg
    14521 tatacgtagc gcaaatgaac tttctgatag ctcagccgtg gtgagaatgt attcgaaatc
    14581 accttcttca cgaagtagct cagcgcttgt gaaagcccct gcttgtacgt agcttagaac
    14641 aggcaccctt cgggctccgt agttgaaatc tgtaacaaca tcgcccccaa gaagtagcca
    14701 tttaggatcg cattcaagag ctgacgctaa agcaagaaga ttgcgtggtt taagtgtttt
    14761 cccgttctct atagcttcaa tcgactgctg gctaatgccc gctcgtttcg caacttcgac
    14821 ctgagttaag tttagttctg ttctgcgttt tttagctcgg ttggcaagat tcatgttgac
    14881 tccttttctg tcattgatga ttacagatac ttctgt
//
LOCUS       DAZGBC010000121        14642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGBC010000121 DAZGBC010000000
VERSION     DAZGBC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.122"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..255
                     /locus_tag="ACHK1J_003569"
     CDS             <1..255
                     /locus_tag="ACHK1J_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7912794.1"
                     /translation="GVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIHA
                     TGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGETSVTD"
     gene            complement(424..891)
                     /locus_tag="ACHK1J_003570"
                     /pseudo
     CDS             complement(424..891)
                     /locus_tag="ACHK1J_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(940..2234)
                     /gene="insG"
                     /locus_tag="ACHK1J_003571"
                     /pseudo
     CDS             complement(940..2234)
                     /gene="insG"
                     /locus_tag="ACHK1J_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2348..2923)
                     /locus_tag="ACHK1J_003572"
     CDS             complement(2348..2923)
                     /locus_tag="ACHK1J_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7912795.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFSGGLSALASQHGVNV"
     gene            complement(2991..3569)
                     /gene="terD"
                     /locus_tag="ACHK1J_003573"
     CDS             complement(2991..3569)
                     /gene="terD"
                     /locus_tag="ACHK1J_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO7912796.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(3618..4658)
                     /gene="terC"
                     /locus_tag="ACHK1J_003574"
     CDS             complement(3618..4658)
                     /gene="terC"
                     /locus_tag="ACHK1J_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO7912797.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            complement(4677..5132)
                     /locus_tag="ACHK1J_003575"
     CDS             complement(4677..5132)
                     /locus_tag="ACHK1J_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7912798.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            complement(5155..6312)
                     /locus_tag="ACHK1J_003576"
     CDS             complement(5155..6312)
                     /locus_tag="ACHK1J_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7912799.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(6312..6893)
                     /locus_tag="ACHK1J_003577"
     CDS             complement(6312..6893)
                     /locus_tag="ACHK1J_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7912800.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            7213..8271
                     /locus_tag="ACHK1J_003578"
     CDS             7213..8271
                     /locus_tag="ACHK1J_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO7912801.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKLFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNNQWFEEHRTAIESTGATLT
                     TGATGIDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTRLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSALPMAVFNHPEHRLVSPQLYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNQGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNLLN"
     gene            8281..9423
                     /locus_tag="ACHK1J_003579"
     CDS             8281..9423
                     /locus_tag="ACHK1J_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO7912802.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            9416..10189
                     /locus_tag="ACHK1J_003580"
     CDS             9416..10189
                     /locus_tag="ACHK1J_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912803.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            10191..11270
                     /locus_tag="ACHK1J_003581"
     CDS             10191..11270
                     /locus_tag="ACHK1J_003581"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO7912804.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            11270..12226
                     /locus_tag="ACHK1J_003582"
     CDS             11270..12226
                     /locus_tag="ACHK1J_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO7912805.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            12237..13332
                     /locus_tag="ACHK1J_003583"
                     /pseudo
     CDS             12237..13332
                     /locus_tag="ACHK1J_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13350..13817
                     /gene="terW"
                     /locus_tag="ACHK1J_003584"
     CDS             13350..13817
                     /gene="terW"
                     /locus_tag="ACHK1J_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO7912806.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            14142..14642
                     /locus_tag="ACHK1J_003585"
                     /pseudo
     CDS             14142..14642
                     /locus_tag="ACHK1J_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         3667 a         3787 c         3635 g         3553 t
ORIGIN      
        1 ggggtgaaga tagaaggatt tacggaccat cttggcgggc aggcgtataa ccggctcctg
       61 tcagagaaac gggcggaatc agtcaggggg tggtttattg cccggggatt cagggctgaa
      121 aggatacatg caacgggccg tggatatgat gcaccagtgg tgttctgccc ggatgtggag
      181 ggcgaagcac tgaaggaatg tctgcgcccc aaccgcaggg ttgaaataac cggggaaaca
      241 tcagtgactg attaacaata aagtgccccg ggagctaatg ccagtcagtt cagcaactga
      301 ctggctcttt ttcggggctg tggggtattt ccagggagcc tgtttaggaa tctgtgtaaa
      361 tgccttttct cagaagtgac cgtccaggcg gtcaccgaac tcgataataa agcggctcat
      421 tgccattcgc cggttttgcc attctacaac ctgctccatc acggcatcgg tgacctttga
      481 tatcagtgcc ggtgaaacat ctgcgtcata cagctctttg aacgcagcag cgatctcacg
      541 ggtggtcatc cctttggcat acaacgagaa gatctggtta tccatcccag taatacgggt
      601 ctgatttttc tttaccagtt gtggttcgaa ggtaccgtca cgatcgcgcg gagtacgcag
      661 ttccagtgga ccgtcgcctg tgataacggt ctttgtggaa taaccgttgc gggagttagt
      721 tcccggtttg gactgatttt tctcataccc aagatggtgt gtcatctctg cattgagagc
      781 ggcttcaacg ctgatctttt tcagtagacg gtcaaactgg ctgaggtctt caggggtttt
      841 gaggtttttg gccagttcgt tagccagagc ctgtaactgt ttttcgtcca taaattaacc
      901 ttcatttgat gcttgattga acatatcaaa atcaggcaat tacacaaatc tatgtacagg
      961 ctctttccaa ggcctctcct ttaccactct cgggaagccc ctttcccttc ttgtcggtaa
     1021 tttcacaagt tgtcccatac ttgcaagatc gcgcatcagc cccggtatac gtcccggtga
     1081 agcgccctgc aatgtcatca ccattccgca tgattctgag aaactcagtt gattcggcca
     1141 gtaacctttc agatgttccg ccattttaat catctgacat cttaccagat tataagccag
     1201 taagacaccc cacagctctt gctccacaag ctccggcttt ttacagcgtc agcctgctca
     1261 gttgcatcgt ctgttttatc tccctgtatc ccagttcaat ttcccggcga tgactgtaca
     1321 gatccgccat ttctcccccg gggaagcgca tggcgtccgt catcgacgtc agcagatggc
     1381 agacttctcc tttgcgcgtc acggtcagca ggcgggctgt cacctcattt cccagtcccg
     1441 gccacttttt tcgtgcctgc gggctggttt tcagcttcac cagatgatcg cctttaccca
     1501 gttttctgat ctcttcatat tgcgctccct ttctgagagg gatcatccag tggcggtgtt
     1561 ctcccaccag gctccaggca tttaacagtc ccagtgagta ataaccttta tccattaacg
     1621 tcagggtgtt atcgccggtt tgttctataa gttgctcagc aagctcattt tcgctgttct
     1681 tcatcgtgcc gaaggctcag ccgtaagcag atggctggtc agttccatct ggcagactat
     1741 tttgacctgc gggtagagcg ccgggttgcc ggcatgtatc tggcggggga aggctgcatc
     1801 gttctctggt gtatccggtg tgcgccagaa cataccatcg atggccagca gggtcaggcc
     1861 gcatcagtgc ggatgcggcg tggcgttatg ccagagctgc gctgttttcg tgaacacgcg
     1921 gcggacagcc tcacttccca ggtgctggcg ggcctgaata acggcactgg gggcaacgaa
     1981 ggggcgattg ccggcagcat gatgtccagg cgattcacaa tctggtgaag gggctcttta
     2041 cgctcaagcg ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg
     2101 cgtagcgtta ccgtacctga ttcggcaagg caacgagaga tgagttcggg gtcgaggtaa
     2161 tcccccagag aagccagtgg gttacgcaga gaatcgtaac gggataccag atcaagggcc
     2221 tgtccagtgt gcataaaaaa tccggaaacg agtgagcgtt tccggattct tacacaacca
     2281 ctggatcggt caactgatcc ttaaccgatc ggcattacgg cattgccggg gtcagactaa
     2341 gcatctttta gacgttgacg ccgtgctggg aagcaagcgc tgacaggcca ccggaaaaac
     2401 cctgaccgac agctttaaac ttccactcag cgccatggcg atacagttca ccgaagacca
     2461 ttgcggtttc agttgaggcg tcttcagaca gatcgaaacg ggcaatttcc gcgccgttat
     2521 cgttgttgta aacgcgcatg aagctgttgc tcaccatgcc gaaattttgt ttacgcgctt
     2581 ctgcatcata gatggtaacg gcaaatacca gttttttgac gtcaactgag acttttgcca
     2641 gatcgatttt aatttgctca tcgtcaccat cgccttcacc ggtacggttg tcaccctggt
     2701 gctctactgc gccatcaggg ctggttttat tattgaagaa aatgaaatgg gcatctgaca
     2761 gcactttacc gtcttcacct actgcgaata cggaagcgtc cagatcaaaa gcctgaccgt
     2821 cagttacacg ggcatcccag ccaagaccaa ccatagcaac attcatggtc ggtgcttctt
     2881 tggtcagaga tatgttgccg ccttttacga gagaaactgc catttttaac tcctgcaaac
     2941 agttgaatga gggctgaatc aacagcccgg aatgaaaagt tagttttcga tcaagacgcg
     3001 ttaatgccgt actgagcaca tacagatgcc agcccccccg cataaccctg acctacagca
     3061 cggaatttcc actcaccatt gtgacgatac agttcaccga acagcatggc ggtttcagtg
     3121 gacgcatctt cagtcagatc gtagcgagca acttcagtct ggttatcgtc attaaccagg
     3181 cgaataaacg ccccggaaac ctgaccaaag ttctgacggc gagcctgagc atcatggatc
     3241 gtcacaacga agatgatcct gtcgatgtca gacgggacag tatcaagttt aattttcagc
     3301 gattcatcat caccatcccc ctcaccagta cggttatcgc cggtgtgcgt tacagaaccg
     3361 tcggatgacg tcaggttgtt atagaagatg aaatctgaat cgccacgcac tttgccgttt
     3421 gcggtcagca ggaatgctga agcatccaag tcaaagtcct gaccgtcagt tgaacgtgca
     3481 tcccagccaa gacccaccag gacgtttttc attgacggag ctgctttact cagggagacg
     3541 ttcccgcctt tggaaagaga aacactcata aaataacctc ttcgattagt aattgctccg
     3601 gttaacactt aaggggatca gttccccttt tcctcagatt caggtttgcc ggggaaaata
     3661 acgcttgcga gaatgcccag caccagaacc cccagtacaa catacaggct ggttgtcgca
     3721 gcgatgctgt aaccatgatg ccagatgtga tcggtcgcgt tcaggcccag ttttaccgca
     3781 atgaagaaca acaacacgat aacagccttt tccagatgaa tcaggtactg tttcagtgct
     3841 tcaaggacaa agtacagagt acgcagcccc aggatagcaa acatcatggc actatagacg
     3901 ataagcggtt cacgactgac agcaatgatt gccggtaccg agtcaaacgc aaacataaca
     3961 tccgagagtt caaccacggc cacacacagg aacagagggg tcgcatacag cgccgccttt
     4021 ttaccacggt caatggttac atcactgttt tctggtttcg ccagttcagc atccacttcc
     4081 ttctggttaa gcaggaaggc atgcccacgt agcttcggcc agacagggaa gaaacgttta
     4141 accatgcggt aagccagatg ctgggagtaa tcttcaattt catcatcatc atcaccgctt
     4201 ttaagcatca tgaccgctgt ccaggcaacg atgatagcga agacgacttc aacgtacggc
     4261 cccagactca gcaggctcgt accgatggcg acaaagattc ccctgaagac gatcgctcca
     4321 atgatccccc agtaaagcac acggtgccga taacgatccg gaacagcgaa ccaggaaaag
     4381 atggccatca tgacaaacag gttgtcgacc gacagtactt tctccagcgc ataccccgtg
     4441 acaaacaaac tggcaacttc agcaccgtgg tggatataga ggaaaccggc aaatgccatc
     4501 gcaacaacaa cccagaatac ggaccagagc gccgcactct tcagggagat tggcttgtca
     4561 tcacggtgca taaacaggtc aataaagatg gctccgactg aaagagcaat aaaaacaatg
     4621 accgtttcag tcgggaagcc gatgtgtgtg gaaaccatat tttaccctta gggatgtcag
     4681 aggccaaatt cagccggctc gaagcccaac gcgatagcga tctcgcggga ggccagtttc
     4741 tcgtcctgat cgaagttacc gtcgcttttc gcaacagcaa taccaacacg taaggccagt
     4801 tgagcggcct caggctgatc tttcagcgcc aggatgtatt tcatggtctc gcccttgccg
     4861 atttcaacat cgaaatcgaa gcttgaaacc agtttattga agaactcgat cacctcattg
     4921 gtatcgaaga ccttcagctc ttctgaagag cgcagaaagc ccatcatttt ctgcttttct
     4981 tccgaactca cgccgtcact cgatacggca atacgggcac atacagcaac ggtgccctgc
     5041 atgaattttt tgtttttgaa acgaccaacc tggcgggtca gttcgtcacg accagagtta
     5101 atggcacctt taactttgtc gaaaaagctc ataccagttt cctttattgc tgtgttattt
     5161 agaaccggcg ctccagcgaa atccccagcc aaatgcccgg tccatttcgt cctggccttt
     5221 gaagtactgg ttgatacgct caactttaat cgcaccgttt tcgtttacga gtctggcaat
     5281 ggcgcacaat gtgcgacggt tttcaccttc agtcaggcgg gtctcaatgg gcggttgatc
     5341 cggcacataa atggtgacca ctccatcagt cttgtcccaa ctgggaacac cttcataaat
     5401 aaaggcgtaa atcagcactt cgcggatatg cttccattca cgtccattga tatgcagcca
     5461 ctcgccgtct gatacatcac ccgtccggtc atcgcccttg agctgaacat aaggttcatc
     5521 gcgataatca ccgaatgcat taccgagcgc ctgaatgacc gacttatacc cgtcctgaag
     5581 ctcaacaaag gctcccagat ccaggtcgat gcctttggag ccgaacatgc ctgcaaagcc
     5641 ggatttgccg ctgccccggt gccagttcag gttgatcctg atttcaccga agttatcccg
     5701 tttggtcaga ctgattgcag gcatctcttt tgtcagagat actttactca ggctgatttt
     5761 tgtctcagct ggcagagggg gtacaaccgg aggagtcggg gctgcgggag ccggttcgtc
     5821 ggcaatattt acgccgaaat gctctgccag cggtttaagt ccaccgttga acccctgcgc
     5881 aacgaagcgg aatttccagc cattattacg acggtaaaat tcacccagaa ttaaggctgc
     5941 ttcctgacgg ccgctcagtt cgacacagcc gctcaccagg ccggtagccc cttgctccac
     6001 gtcgatggaa agattacgaa gaccggacac cgtctgccca ccgtcacagg tcacggtgaa
     6061 cgcaattttc tggacatcag gtttcagccg gttgagcgcg acagtaaagg ttgagtactg
     6121 accttcactg accaggctga cagtaccatc atcgttacgt ggctgaccat aaaacaccat
     6181 gtccgcatcc ccctgcacct tcccggcggc atacaggcga aacgctgaag catccacctg
     6241 gacgcccgag gttatccgga cccgcaactc ctgggctgga acgggggcat tgccccccgg
     6301 catcaggttc attagcgaat aaccgcagaa acgatatcgg agtgcatatc gtcaattgtg
     6361 cgaccgcgac aggcatgacc aagggcggta aagtcccagt tgccgttatt acggcgcagc
     6421 gaagaaataa cgatcccggt atgtgagccc tgctcagtca gcttgtagca agccagctct
     6481 ttgccggttt gatcgaccac acgacagaac gcattttcga cgtcattgaa cgactgccca
     6541 cggaagctgt taaccgtaaa cgccaggtat tcaacgtttg caggcagacg ggagaggttg
     6601 accttaatca cttcatcgtc gccgtcaccc tcaccagtca ggttatcacc gctatgcaca
     6661 acagctttgc aggtggattc cagtttgcgg aaccagatgg tgtcaattgt tttaccggtg
     6721 ctgtccatta atacacagcc agcatcgaga tcgattgagt cgttacctcc aaacaatcct
     6781 ccaagaaaac cttttttctt aaccggatcc caaccgagac cgaaatgaag ctggcttaat
     6841 gcaggtgatt ctttgctgag tgataccgtc tgattcttaa ctaatgaaac catgttttat
     6901 tccttaaaat gctgataact gagacaatca aaagtatatt tagtaactgg cgaagaaaat
     6961 catatcatga tgaagttctc tgacaaggga aatttggtag ctttctgtaa aaacacaaag
     7021 agaaatcctt ttcatgatgt cctttcatct aaataccaat taaatatcaa cacattaaca
     7081 ggtaacacaa aaacactgat acactccata acctcaggtg ttattaatca tattatgatt
     7141 gacatgtagc atatacaccc ataaactcac ttctcgacaa taaattttct tgccgttccg
     7201 gataataaaa aaatgaacaa aaaaatttgg tttatggaag gtttgtcctc tcagagagat
     7261 attattcagg gggttaaatt atttgcacaa aaaaataatt ttgccattac cgtttttgcc
     7321 tcccaccgta acgaaagaaa tgaaatcctt tccgttgccg attattcttt gactgaacct
     7381 gaagatcctc aaaaacgtct tcagtttatt caggaaacca ttcagaccta cggcatccac
     7441 catattcata ctggtcgtaa caaccagtgg tttgaagaac accgaaccgc cattgaatcg
     7501 accggtgcca cccttactac cggtgcaacg ggcatcgact ggttaactct ggctgacgaa
     7561 aaagttactt ttgctcagtt tatggagcaa aaggggctcc cggtcgtacc atcctggcgg
     7621 gtgaatacgc tggcagaatt aaagacacgc ctcgcggctc cgccgttcac tgacagtccg
     7681 gtatgcgtga agccggtgac gggtatctac ggcatgggat tctggcgctt tgatgacagt
     7741 gctttgccta tggccgtctt taatcacccc gaacatcgtc tggtcagccc gcaactgtat
     7801 attgctgcag cgtcggcttc tgagtcgttt aaaccccttg ttttgatgcc gtacctgcct
     7861 ggcccggaat tttccgtcga tatcctcgcg gataagggcg aaatactcgc agccgtggga
     7921 cgccgtaagg aaggggctat ccagtatctg gtaaaccaag gaagcgcctg ggaactggcg
     7981 tgtgactgcg cccgggttat gaaggccgac gggctggtga atgttcagac gcgaaacgat
     8041 gtgaatggca acccggtgct gcttgaaacc aacatgcgtc cgtcaggcgg ggtgggttat
     8101 acccttcaca gcggggttaa ccttcccggg ttgtttgctg cttttaagct cggtctgatg
     8161 tctgaagatg cggtgatcca gagcgccaga aatacctttt ctccggttgc agtgagatcc
     8221 atcacagatg tcatcgttta cccggaatca ctctctaacc ttctgaatta aggcgtgttt
     8281 atgtctgata ccctcgaaga tatgatttat cgccgtaccc tctcatgcgg tacgattcag
     8341 gtaacccgcg accagggtga agtttcgctc gatgacctgt ttgatatcgc tgaaagacgt
     8401 aacccgaaac gcgcctttct gtttgtcagc aaagtactcg gcaggcacat tcccgtgcca
     8461 ccctcagtca tgcggcaggc ctacagacag cttgccagcc agttcccctc aacgctgaca
     8521 gggcccatac tgtttatcgg tatggcagaa accgccgttg ggcttggggc tggtgtgttt
     8581 gatgaagtgc gccaccagca tcgtgaatct gtctatctga cctctacccg tcacccggtt
     8641 gatggcacat tgctttgcga gttcaaggaa gaacacagcc acgccaccga tcatctgatc
     8701 tatctgccag acgatgaaga gaaaagacgt cgtgttacaa acgcgcgaac tctggttttg
     8761 attgatgacg aagcaaccac cggtaatacc tttattaacc tgctttctgc cctgcgtaat
     8821 accggcaagc ttcaacatat tgaacaggtt atcgctgtta cgcttacgga ctggagcgaa
     8881 aatgccctgt ccaggcgcag cactttaccc gtcacttcgg tttctcttgt aagtggtcag
     8941 tggggatgga ctccattacc agatgcacct gtcccggaca tgccgaaggt caacgtaact
     9001 tcacgtggaa aatgggacat ccagggaaaa cagtcctggg gtcgactggg gatgctcgca
     9061 cctgcggccg atctcggtca tgaggtatcc ttccgcaagg gggagcgtgt tctggttctc
     9121 gggacagggg aattcgtctg ggagccgttc ctgcttgctg aacggctcga agctgccgga
     9181 gcacaggcat tttatggtgc gaccacccgc tcccctatcg ccgttggtta tgccattaag
     9241 tccgcaattt cctttacgga taactacggc ctgggcatcc ccaattttgt ctataacgtc
     9301 gcccatcagc aatttgaccg cattcttgtg tgtactgaga cacctgcaga aagtattgac
     9361 acgcggcttc ttaaagcgct ggctgaggtt gcacccgtcg tggagattgt tacctatgaa
     9421 taaacccgtt gttctgagcg accttgacga cacgctcttt cagacccgtc gtaaaatggt
     9481 agacgagctg gccctggagc cattccgtac cggtgccgtt gatcgtactc tgaatccacg
     9541 aagctttatg acagaagaac agtccatgtt agtggactgg ctcctagagc aggctgaact
     9601 cattcccgtt accgcacggg gaactgaaga aatcagccgc gtaaggatcc ctttccactc
     9661 ctgggcaatc accacgcacg gggccgtcat tctcacgcct gaaggcaaac ccgacgagga
     9721 gtggaaggcc catatgctcg gccagctaac tccctaccag gaaaagctgg catcgatgca
     9781 gcgtctgatc actgaaatga tggacgcaaa agggatcaat gcctgggcaa gactgaactt
     9841 cgaatacggt gaaacggcgg tttatatggt gatgaaacac cgcgacagca ctcgccttga
     9901 cgaactcaat gccattgcag atgagataga aacggtattt tcgaccgagg gcttctacat
     9961 ccaccgcaac agtaacaacg tagcctggct tcccaccccg gttgagaaag ggctggccgt
    10021 cagctggctg cttgaaaaac ttcgggctga acgcggagtt ttccccgtaa ttggtctggg
    10081 cgacagcctg agcgatcatc gttttatgaa actgtgcagc tggtttggca tcccgcgtca
    10141 gagtcagttt gcagatgcca tttcacagcg aatttttgga gaaaattaaa atgcagccac
    10201 atgaaacatt taccggctca taccagcccg gtgacgtgga atttctgcta aagccggttg
    10261 tcattgaaat gacgccagtt gatcaaaaag aagagctgat tcagtccggg aagaaacatt
    10321 attcggacat gctcagccag gagccagcgc cgacacaatg gcatcttgat ttgtttcacc
    10381 gggcgctgga tcggggcgca gagagacttg caaaagaagt cacacagctc gctattgctc
    10441 tggccgaacg cttcggtgat gagcccatag tactggccag tcttgtcagg gctggcgtac
    10501 cgctcggcgt tatgctacac caggctctgc gtgacatggg aaaaacctca tggcattacg
    10561 gcatcagcat catccgggat cgtggaattg acggtacagc acttgatatc attgaagagc
    10621 ggcatggtac cagcggcatt gtctttgttg acggatggac aggtaaaggc gcaattaccg
    10681 gagagcttgt acgcgcactg agagatcgcc cgggctatcc tgagcagccg cgactggttg
    10741 tcctggccga tccctgtggc tgttcctggc ttgcagcaag tgatgatgac tggctcattc
    10801 cttttggcat catgggggct ccggtgtcag gcctgctctc ccggtcagta tggtcctctg
    10861 aaggattaca tgggtgcatg gtttgcgagc atctcagtga attcgaatgc agccggatgc
    10921 ttgtcgatac cgtcgctcat ttccgtaaga agttaacacc gtcatccctc gctcctctga
    10981 gctggaatac ggagtcaacc cgggtcttat ggcagacaag tcgcgacgtt atcgcgttcc
    11041 tggccgatga atttaaagtg gacagcgtca accgtattaa acccggtatt gctgaagcaa
    11101 cccgggctgt attacgtcgg gtaccggacc atgtatttgt gcgttcgatt gacgacccgg
    11161 acgttgcctt acttgtgggg cttgctcgtg aaaagggaat aattgttaca gaaatggggg
    11221 gaaccctcgg ccagtatcgg gctgtaacca ttatcaaaaa ggtattctaa tgaaaaatcg
    11281 tctttctccc tggaatcttg gtgccactct atacatgcct gcaacacggg aagatattgc
    11341 tgatgccgtt ctgcacgaga aaatcccggg tttgcgctct ctggtgattt gcctggagga
    11401 tgctgtcagt gaagcagaca tccccgtcgc cctcaaaaat ctagagcacc tgctgcacga
    11461 actcagtaac agcatgcgta gcctgggtaa aaataactgg cccctgttgt tcattcgccc
    11521 ccggcatgcc gaaatgggca aatggttaaa agaacattat gatctttccg ctgtcgacgg
    11581 gtttgtgtta ccgaaattta ccctaagttc gttagctgaa tggtgggata tcatgggcgg
    11641 aactcacctg agcatgatgc caacgctgga aacagaaaac gtctttgatg tgattcagat
    11701 gcgcgagctg gccacccggc tgttggaaca tccctgccac aagcgcatta ttgcgcttcg
    11761 aatcggcggc aacgatctta tgaacgtcgt gtcgcttcgc cgtccccgag ccctgacgct
    11821 gtatgacagt ccgatgggct acgtcatcaa aatgctcgtt tcagtcttca gcccgcgtga
    11881 ttttgccctg accgcgcccg tatgtgagca tattgacgat catgccgtta tggccagaga
    11941 gctggctctt gatatggcac atggacttgt tggaaaaacg gccattcatc cagatcagat
    12001 agaggtcatt caaaacgcgc tgatggtcac tcagggtgag tactctgacg ctctgcggat
    12061 cctgaactct acccaggccg tgtttaagtc gcagggggcg atgtgtgaac ctgccacaca
    12121 tcgccgctgg gcggctggca ttctggacag agcccggttt tacgggttac ataacgagca
    12181 aagcgctgat ggaatcagat tacttactat aacccagcat cattaaggaa atcataatgg
    12241 ggttcaggtt cagaggtact tccgtttctt tctgtagccg gggggcctat gccaatctgg
    12301 ggttacccgg taccgagctg agttaccgta cccggtttga gctggccgcg ccttccggag
    12361 gtggaaacag gacagcaacc gtcccggatc tcagacagtc gcttgagcag gaagcagttg
    12421 aactcatgtc agcggtgacc tcaattcgta acattcacga gttgacgctg gatccaaaaa
    12481 caggcatcaa ttgggcagag ctggaagcag tatacctgca tagcagaaca tcgctttttc
    12541 aggttccggc accagtgcgt cctgaaaaac cagaatacca tgcattgtcg gaaaatcctg
    12601 cagggaacaa aggcattgtt tttctgggta aatggtttga atcggaatca gctaaagctg
    12661 agcgtcaagc agggaatctt cgccgatggc ggcaagaact gattgatgtg aagcgtgaga
    12721 atccccttcg acagcaccgg taccagcaac ggcagacgac cagggccgaa cagtattcaa
    12781 actggaagtg tgatgctgaa gaacatgaaa aaaggctcgc cacgactcag gcagatgccc
    12841 ggcagttgtt ccgggcagac gcagcgtttt tcgaatcata ccttgcgggt gtgctttcag
    12901 aaactgaatg gccgcgtgaa acgcttgttg catttgaagt aaagccggag atatcaacag
    12961 tcctgccgga tgttggttta gctgagattg aagatttccc tgataagatt tacggcgtca
    13021 atgcccgggg catggagctg acgcaaaaag ccatacgcga aaactatgcc cgccacagcc
    13081 atggctgctt gttccgactg gtcggtatcg tgttacatac gctacctttc gacaaggtga
    13141 tcgcatcagg atttacacaa cgagtaagcg gaacgactat ctggaggatg tttatatcct
    13201 gtcctgcaaa tgcactcgca gccagaaggc atagaacata ttgatccggt tgaagcgtta
    13261 ggctatcacc ctgttattcg aaagatgaga aacacattca tttttcagcc tattgaacca
    13321 ctaacccttt aagcaaacta aatattacga tgcaattaaa taccagacag gccagaattt
    13381 ttaagcttgc caacttactg ggctccggca agcctgtttc cgctaccgac attataacct
    13441 atctggactg ctcagagccg acattaacac gggccctcaa agagctacga aagtcatatt
    13501 ctgcagaaat caaatacagt aaggcaggtc attcctatca tctcgttaat ccgggtcaac
    13561 tggataaaaa gacacttcgc cggatgaatg aggcgcttgc acagaatgct gatctgaaaa
    13621 ccggtgagtc tacaggaaag gtcgtactcg ataaggataa aaaaacagcc gtatcattat
    13681 cgctacgcat gcgaatttta agaaaaattg accgtctggc tgcactgagt ggatcgaccc
    13741 gaagtgaagc tgttgaaaaa ctggcactgc attccgttga cgaactgata aaagaataca
    13801 gcgctaaaaa atcctgatga caaatctcag ctcggggtga ataccccggt tttttcatat
    13861 ttcaactctg tcttggcgtg acgtattttt gatctattct cctcccgcaa tgccagcagc
    13921 tccttacagc ctacaaaacc tagcgtcttg acatcctctt gtgctacgca tcaatctttg
    13981 ggcgatttac actccactga ctgccgtatg tagccattaa gccagtattg gtaacgtaga
    14041 tgcccacctt ttcacttatt tggtggacac cactttgtct aactcgtcag attctgacca
    14101 aacgtttcag gctgtacgct tactctgaag gggggagaat agtagcccgt gatagcattg
    14161 agctaactga cagccagatc gcagcactaa agcgtaaagc cagtgatgac gaggcctgtg
    14221 gcgaaagcga aacggctcat ccgggatatc tgggttcgca ggacacgttc tatgtaggca
    14281 acctgaaagg tgtcgggcgt atttatcagc agacgttcgt tgatacgtac tcgaaagtgg
    14341 ctcactgtaa gctttacgtc accaaaacac cgattaccgc ggctgactta ctgaatgatc
    14401 gtgtactgcc gttttatacg tctcagggcc tgccgatgct gagaatactg accgacagag
    14461 gcacggagta ctgtggcaaa gtagagcagc atgattatca gctttatctg gcaataaacg
    14521 atatagaaca cacgaaaaca aaggcgatgt ccccgcagac caacggcatc tgcgagcggt
    14581 tccataaaac aatcctgaac gaattttatc aggtaacgtt ccgtaaaaaa ctgtacggcg
    14641 at
//
LOCUS       DAZGBC010000122        14514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGBC010000122 DAZGBC010000000
VERSION     DAZGBC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.123"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            261..467
                     /locus_tag="ACHK1J_003586"
     CDS             261..467
                     /locus_tag="ACHK1J_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912807.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            1020..1514
                     /locus_tag="ACHK1J_003587"
     CDS             1020..1514
                     /locus_tag="ACHK1J_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO7912808.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1514..3616
                     /locus_tag="ACHK1J_003588"
     CDS             1514..3616
                     /locus_tag="ACHK1J_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7912809.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSHSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            3613..3825
                     /locus_tag="ACHK1J_003589"
     CDS             3613..3825
                     /locus_tag="ACHK1J_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912810.1"
                     /translation="MNQNDIEAMIQRYMEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3825..5333
                     /locus_tag="ACHK1J_003590"
     CDS             3825..5333
                     /locus_tag="ACHK1J_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7912811.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFVQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKDYEDYELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5278..7305
                     /locus_tag="ACHK1J_003591"
     CDS             5278..7305
                     /locus_tag="ACHK1J_003591"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO7912812.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7392..7715
                     /locus_tag="ACHK1J_003592"
     CDS             7392..7715
                     /locus_tag="ACHK1J_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO7912813.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7708..7983
                     /locus_tag="ACHK1J_003593"
     CDS             7708..7983
                     /locus_tag="ACHK1J_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO7912814.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQHWTVTRHERFNGKPMIFIE"
     gene            7995..8573
                     /locus_tag="ACHK1J_003594"
     CDS             7995..8573
                     /locus_tag="ACHK1J_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7912815.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLTRR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLVNGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8570..8971
                     /locus_tag="ACHK1J_003595"
     CDS             8570..8971
                     /locus_tag="ACHK1J_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7912816.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8982..9725
                     /locus_tag="ACHK1J_003596"
     CDS             8982..9725
                     /locus_tag="ACHK1J_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7912817.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEICAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASINVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            9786..10172
                     /locus_tag="ACHK1J_003597"
     CDS             9786..10172
                     /locus_tag="ACHK1J_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7912818.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAGEG
                     ELSEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDDEHEALTPEKS"
     gene            10181..10510
                     /locus_tag="ACHK1J_003598"
     CDS             10181..10510
                     /locus_tag="ACHK1J_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7912819.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10482..13547
                     /locus_tag="ACHK1J_003599"
     CDS             10482..13547
                     /locus_tag="ACHK1J_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7912820.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKSAQAHERMAREVEKTRQRMEALSQK
                     MRDEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSSGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSYMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13547..13876
                     /locus_tag="ACHK1J_003600"
     CDS             13547..13876
                     /locus_tag="ACHK1J_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7912821.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13886..>14514
                     /locus_tag="ACHK1J_003601"
     CDS             13886..>14514
                     /locus_tag="ACHK1J_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7912822.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSTSFVLSTPTETDG
                     SVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCL"
BASE COUNT         3554 a         3351 c         4337 g         3272 t
ORIGIN      
        1 tgctattcaa caatacaaat tacccataaa cctcgttttt acggggtttt gttatattta
       61 aacattaccg aatagatata aatctcgatt gtcgatggta ttggttgtgt gacaatacct
      121 agtctttccg gtatgcctga agagaataca aacgacagat tatgtaaggg aaacgcgctg
      181 aaactttcac tctttcatgc tgagtgattt gacgttatat ttttagccgc aagtgaagag
      241 caaacgcatg gagcgacaaa atgcataaag atcaatacac tgatgccccc tcccaggagc
      301 aggttcgtgt aaaaactatg cttaatagca ccatttcgat gggttacccg gatgttgtaa
      361 ttgcatgtat agaacatcaa gtatctctgg aagcattcag ggcaattgag gccgcgttag
      421 taaagcacga taagaatacg aaggattatt ccctggtggt tgactgatca ccataactgc
      481 taaccattct gaccatttca caagtgacag agtcaatatc gtattctgtc actgtcaggc
      541 taatacagta ctgcaattca actacagcaa tgcctcgtat ttaggtgaat ttacaatatc
      601 gtccagttcg gatgccggct gcatttctga agataaggca tttcggttcg cgtattttcc
      661 cctcatgctc gtcagtcctg cgcgtagtaa gaaacaggac actcacacta atttgtgtgg
      721 gcatgctgtg atgtccttct gaattatccc cacgccatta tgtaaagctc tgtttcagat
      781 gctcgtcacg gttgtcaggc tgtcgggtcc tccctgtggg gggccctgcc acggggcggg
      841 agcgtcgcgg aaaaaggcta gtttttgaaa tttcattcgt catcaccacc actgtaatag
      901 attgatatta cagtggtttt atttttatgg tgtcgatttt gattgttttt tgttcatcac
      961 taacaccgtt tgcctaaagt tgttcgcaag atgcatgttt aaaacattct ggagcgggta
     1021 tggatcgaga gttaaaaaat ctgacgctga atatcagtca actggcggca ctgtcaggtg
     1081 tacatcgcca gactgctgcg gcaaggctgc aaaatctacc cgttgcaggg gggcatgaaa
     1141 gcaacctcaa gctttatcgg gtggttgata ttgtgtcggc atttctggca ttaccaccgc
     1201 cggttgcaga aggcgaaatg gacgcgcatg agcgcaaagc ctggtatcag tctgaacgtg
     1261 agcgtcttaa gttcgaacag gaaacggcac aactcattcc ggccagtgat gtcagacggg
     1321 agtttgccat ctgggcaaaa gcggtcgtgc aggtgctgga gacattaccg gatattcttg
     1381 aacgtgactg cggtctgcag cctgccgctg tgagccgtgt tcagtccatt attgatgatc
     1441 tgcgcgatca gatagccctg cgggtgactg aagcaggtgc ggatgatgag gaggaattac
     1501 agcaggagga gtaatgctga atcaggaaac cgcaaaggca gcacgaaccg attcaggtta
     1561 tatccttcgc gcaccgagac gaatgcgggt tgctgatgcc gttgctcagt atatgcgggt
     1621 gcccatgggg gcagggaact cagtcccgtg ggatccgctg gtggcaccgt atgttattga
     1681 gccgatgaac tgcctggcct cgcgtgaata cgacgcagtg atatttgttg gcccggcacg
     1741 aaccggcaag actatcggcc tgattgacgg ctgggtgatt tacaacgtga tttgcgatcc
     1801 tgctgatatg ctgatcattc agatgacgga ggaaaaagcc cgcgaacact ccaaaaaacg
     1861 actcgccaga acgtttcgcg tcagcccgga agtggtcagt cgcctgagtc cgaacaaaaa
     1921 tgacaacaac gtttatgaca gaacattcct tgctggtaac tacctgaaaa tcggctggcc
     1981 gtcagtcaat atcatgtcct catcagatta taaatgcgtc gcgctgacgg attatgaccg
     2041 ttttccggaa gatattgatg gcgaggggga tgctttctct cttgcctcaa aacgtaccac
     2101 aacatttatg tccagtggta tgacgcttgt ggagagttcc cccggcaggg atgtgaagga
     2161 tgtgaaatgg cgacggactt caccgcatga ggctccacca accacgggga tactgtcgct
     2221 ctataaccgt ggcgatcgcc gtcgctggta ctggccctgt ccacactgtg gtgagtattt
     2281 tcagccctgc ggcgatgtgg ttgctggttt ccgtgatatt gccgatcccg tgctggcaag
     2341 tgaggcggct tatattcagt gtccttcctg ttcaggacgg attatgcctg aacaaaaacg
     2401 tgagctgaac ggacgtgggg tctggttgcg ggatggtgaa tccatcaatg cggatggcag
     2461 tcgttatggt gatccccgac gctcacgtat tgcgtcattc tggatggagg gtccggcagc
     2521 tgcttaccag acactctcgc aactcgttta caaactgctt actgcagaac aggaatacga
     2581 gacaaccgga agtgaagaaa cactcaagac ggttatcaat accgactggg gattacctta
     2641 tcttccccgc gccagcatgg agcaacgaaa aagtgaactg cttgagcagc gggcagagcc
     2701 agttccttcc cacagtgtgc cggatggcgt taatttcctt gtggcgacag tggatgtgca
     2761 ggcgggacgt catcgccgtt ttgtggttca ggtaacgggc tatggcagcc gtggcgaacg
     2821 ctggattatt gatcgttaca acatcacgca gtcattgcgc ggtgacagcg acggggagag
     2881 ccagcgaatt gatccggcca gctatccgga agactgggat gtcctgctga cggatgtttt
     2941 tcataaaagc tggccgctgg cctccgatcc ttctcaacaa atgcgactga tggcaatggc
     3001 ggtggactcc ggcggtgaag acggggtcac tgataatgcc tataaattct ggcgtcgttg
     3061 ccgtcgtgat ggccttggta aacgtattta cctgtttaag ggcgacagca tccggcgcgc
     3121 aaaactgatc actcgtacat tccctgataa caccggacga acgggccgac gggcgcaggc
     3181 cgcaggtgat gtgccgctct ggcttcttca gacggatgca ctgaaagacc gggtgaataa
     3241 cgcgttatgg cgtgactcgc caggtcccgg ctatgtgcat ttccctgact ggctggggag
     3301 ctggttttac gacgaactga cgtatgaaga gcggagcagt gacgggaaat ggagtaagcc
     3361 tggtcgcggt gccaacgaag cttttgacct gatggtgtat gccgaggctc tggtcattct
     3421 gcatggatac gaaaagatcc gctggccgga tgcaccggag tgggcgagcc gggaaacctg
     3481 gctggagtgt gtccaggaca gtaccgaacc gtcatcctca ccggaaccgg tatccacgcc
     3541 tgttaaaaaa caaaaacgga agaaaacagt aactgacgat gttaacccct ggctgacttc
     3601 cggaggatgg ttatgaacca gaatgatatc gaagccatga ttcagcgtta tatggaagct
     3661 gaaatggcgg tgctggacgg aaaatccgtc acctttaatg gtcagcagat gaccatggaa
     3721 aacttatctg agatccggca gggacggcag gagtgggagc gccgccttgc ggctctgatt
     3781 acacgacgac gggggcatcc cgggtaccgg ctggcgaggt tctgatggca attcttgatg
     3841 atgtgattgg cgttttttca cctggatgga aagcggcaag gctgcgttcc cgtgcggtga
     3901 tccaggctta tgaagccgta aaaacgacgc ggacacacaa agcccgacgg gagaaccgaa
     3961 ctgccgacca gctaagccag tacggggccg tgtcgttacg tgagcaggcc cgttaccttg
     4021 ataacaacca cgatctggtt attggtgtat ttgacaagct ggaagaacgg gtggtgggga
     4081 aaaacgggat tattgtcgag ccacatccgg tattacgcaa tggggccatt gcccgtgatc
     4141 tggcagcgga gattcgcacc cgatggagtg aatggtctgt cagcccggaa gtcaccgggc
     4201 agtttacccg tccgatgctg gaacgtctga tgctgcgtac ctggctgcgc gatggtgagg
     4261 tgtttgtcca gatggtttcc gggcgcataa acagcctgac gccttctgcc ggtgttcatt
     4321 tctggctgga ggcgctcgag ccagacttta ttcccatgac cagtgatgag agcaacaggc
     4381 tgaatcaggg cgtgtttgtt gatgactggg ggcgtcccga aaaatatctg gtgtataaaa
     4441 gccgtcccgt atccggacgg cagatggaaa ccaaagaagt ggatgcagag cgaatgctgc
     4501 atcttaaatt tgttcgccgt ctgcaccaga tgcgcgggac gtctttgttg tccggtgtgc
     4561 tgatccgcct cagtgccctg aaagattatg aagattatga gctgactgca gcaaggatcg
     4621 ccgctgctct ggggatgtac atccgcaaag gcgacgggca gagctatgaa ccggatggta
     4681 atggcagcaa ggataaggaa cgcgagctta ccattcagcc aggcattatt tacgacgatc
     4741 tgaaacccgg cgaagaaatc ggaatggtga agtcggatcg ccccaatcct aaccttgaaa
     4801 cttttcgtaa tggtcagttg cgtgccgtgg cggcgggcag tcgtctgagt ttttccagta
     4861 cagcgcgcaa ctataacggc acttacagcg cccagcgtca ggagctggtt gaatctactg
     4921 atggctacct gatcctgcag gactggttta ttggtgccgt cacccgcccg atgtatcgtg
     4981 catggctgaa acaggctgtg gcatccggtg ttatcaggct accccgcgat cttgaccgtt
     5041 cttcactgta taccgcggtg tattccggac cagtgatgcc gtggattgac cctgttaagg
     5101 aggctgaggc ctggaaaatc cagattcgtg gtggagcggc gacagaatca gactgggtac
     5161 gtgctggtgg tcgtaatccg gatgatgtca aacgtcggcg caaggccgaa attgatgaaa
     5221 accgcaagct ggatctggta tttgataccg atccggccag tgataaagga ggcagcagtg
     5281 ccgcaacgaa acgacaggag ccgcagcaca ccgacgacca gtccgaagaa taattcctgg
     5341 ttcaggatgc aggctggtca ccagagtgac gcggatattt atatttatga cgagattggt
     5401 ttctggggtg ttacagcgaa gcagtttatc agtgatctga atgcactggg cgatatcacc
     5461 cacattaatc tccatattaa ttcaccgggt ggcgatgtct ttgaaggcat cgccattttt
     5521 aatgcgctga aaacacatgg tgcgtccatt accgtttatg tcgacggtgt ggcggcgtca
     5581 atggcgtcgg tcattgcgat ggtgggaaac ccggtcatta tgccggaaaa caccttcatg
     5641 atgattcata aaccatttgg ctttacgggc ggtgatgcgg aggacatgcg cacctatgcc
     5701 gacctgctcg ataaggttga ggcggttctg ttacccgctt atgcacagaa aaccgggaaa
     5761 accaccgatg aaattgctgc catgctggcg gatgagacct ggatgtccgg tgccgaatgt
     5821 ctggcacagg gatttgctga tcaggtgacg ccagccgtta aggcaatggc atgtattcag
     5881 tcaaaacgta cagaggaatt taaaaagatg ccggaatcca ttcgaaacat gattactccg
     5941 ccacgcaaca gtgctccacg cgtacaggat gatgaacctg cagcctcccg gacgccagtg
     6001 caggcagcag cacccgtggt ggatgaaaac agtatccgtg cgcaggtact ggcagagcaa
     6061 aaagcgcgtg taaacggtat taatgatctg tttgccatgt ttggcgggcg ttatcagacg
     6121 ctgcaggctc agtgtcttgc cgatcctgaa tgttcgctgg agcaggcccg cgaaaagctg
     6181 ttgaacgaga tggggcgcga gtccacgcca tccaataaaa ataccccggc tcatatttat
     6241 gccggaaacg gtaattttgt gggggacggg atccgccagg cgctgatggc gcgtgccgga
     6301 tttgaaaaaa ccgaacgtga taatgtctac aacgggatga ccctgcgtga atatgcccgt
     6361 atgtcactga ctgaacgggg tattggggtt tccagttata acccgatgca aatggtcggt
     6421 gcggcgttca cacacagtac gtctgacttc ggtaatattc tgctggatgt tgcgaacaaa
     6481 gccattctgc agggctggga agatgcccct gaaacctatg aacagtggac gcggaaaggt
     6541 cagttgtctg attttaaaat tgcccatcgt gtgggtatgg ggggcttcag tgctctgcgt
     6601 caggtgcgtg aaggggcgga atataaatac gtcaccaccg gagataaaca ggccactatt
     6661 gcactggcga cctatggcga gctgttcagt atcacccgtc aggccattat caatgatgat
     6721 ctgaatatgc tgaccgatgt cccgatgaaa ctgggccgtg cggcgaaatc cactattgcc
     6781 gatctggttt atgccattct gacgtctaac ccgaaaatct ccacagataa tgtaagtctg
     6841 ttcgataaag cgaaacatgc aaacgtactg gagagcgctg caatggacgt ggcatcgctg
     6901 gataaagccc gccagttgat gcgcgttcag aaagaggggg agcgtcatct gaatattcgt
     6961 cctgcgttcg tactggtacc gacggcgatg gagtctgttg ctaaccaggt cattcgctcc
     7021 tcaagtgtca agggggctga cattaacgcc ggtattatta acccggtgaa agattttgcg
     7081 accgttattg cagagcctcg tcttgatgat aacagccaga ccaccttcta cctggctgcg
     7141 tcaaaaggct ccgatacgat tgaagtggct tatctcaacg gtgtggatac gccatatatt
     7201 gatcagatgg agggcttcag tgtggatggc gtgacaacga aagtgcgtat tgacgccggt
     7261 gtcgcgccag ttgatcaccg cggtctggtg aaatgtacgg cgtaaacgtc gcagacaaca
     7321 actctgatgg cccgtaaggg ctttttttgt acctgaaatc agcccctgaa cgaggctgtg
     7381 cggagacagt tatggcaaag aattttgtag aagaaggaaa aacggtggcg attgttgcca
     7441 gtgcagccat cagcagcgga gacctggtgc aggtgggtga tgtttttgcg gtggcgctga
     7501 ccgatattcc acagggtgaa acaggcgacg gcctgaccga aggtgtgttt atgctgccta
     7561 agctgaaaac ggatgacatg aaaacgggta agaaggttta tctgaagtcc ggaaaagttc
     7621 agttgactaa cagcggctct gatccgctgg tcggggttgt ctgggcagat gccggaacca
     7681 gtgcagaaga agtgccggta aaactcaatg tctgatccct tttcccggct ggcagcgcgt
     7741 atggatgcga tcacggtcag aaagatggga aagacagcct cgattaatga tgtcgatatg
     7801 actgtgatcc cgggagaaac actggcagag ctgaatgctc tgtccggacc tgcggtctct
     7861 ctggtggtgt tttcttcggg ataccgccca cggcgcgggg atcgcgttgt ttatgacgga
     7921 caacattgga cggtcacacg gcatgaacgc tttaacggta agccaatgat ctttattgag
     7981 taaagaggtg tgggatgaag gggcttgaga atgccatccg caatctgaac agccttgata
     8041 cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag aaagcggtct
     8101 cggttgccac ccggcaggtt gccgggaata ccgttgcggg agataaccag gtgaaaggga
     8161 tacccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg tcaggaaaaa
     8221 tgacggccag gatccgcgtt aaccggggca atctgcctgc cattaagctg gggaccgccc
     8281 gggtcagact gacccggcgt ggtggaaaac tgcagtaccg tggcagcgtg ctgaaggtgg
     8341 gtaaatatct tttccgggat gcgtttattc agcaactggt gaatggtcgc tggcatgtga
     8401 tgcggcgtat tgatggcaaa aatcgttacc ccattgatgt ggtgaaaatc cctctgtccg
     8461 gaccgctgac acaggcattt gaagatgccc gcgaccgcat cattgctgcg gaaatgccga
     8521 aacagctggg gtatgcactg aaacaacaac tgaggttatg gctgacccga tgaaccgaca
     8581 tacacaaatc cgccaggtcg tactggcacg ccttcgggaa cagtgtggag acagcgccac
     8641 gttttttgac ggacttccgg catttgttga tgcgcaggaa ctgcctgccg tggcggtgtg
     8701 gctgagtgat gctcagtaca ccggaaaaat gacggatgaa gatgactggc aggctgttct
     8761 gcatattgct gtcttcatcc gggcacaggc accggattca gagctggata tgtggatgga
     8821 gagcaccatt ttcccggccc tgaatgatgt accggcactt tccggactca tcgacaccct
     8881 gatcccactc ggttttaact atcaacgtga taatgagatg gccacctggg cgatggcgga
     8941 aatcacgtac cagatcacgt acacgaatta aggaggtggt aatgaccaca ccaaatccac
     9001 tggcaaaaac gaaaggtgcg ggaacgacgt tctggatgta caccggcaag ggcgatgcgt
     9061 ttgcgaaccc tttatcggac actgactggc tgcgtcttgc gatggtgaag gatctgcaac
     9121 ctggcgaaat gaccgctgat gcagaagatg acacttatct cgatgatgaa gatgcagact
     9181 ggaaaacgac aactcagggg cagaaatccg tcggtgatac ttcggcgacg ctggcctggc
     9241 gtccgggtga cagcggacag aaaaaactgg ttcagttgtt cgactccggt gaaatctgcg
     9301 cgtttcgtat caaatatccc aacggcactg ttgatgtttt ccgtggctgg ctgagttcac
     9361 tgggtaaaac cattgcctca aaagacgtga tgacccgcac cgtgaaaatc agcggtgtgg
     9421 ggcgtccgta tctggcagag gagggcactg aaaccgtggg cgttaccggg ctgacggtgg
     9481 caccggcatc cgccagtatc aatgtgggag caaccaccac gctgaccttt acagtaaaac
     9541 ctgacggagc cagtgacaaa gcgatcagtg tgcattcgac agatccacag acagccactg
     9601 tgaccctgaa cggacttgtg gccacggtga aaggcgtgaa gcagggcagt gtcagcattg
     9661 tgggcatgac ttctgacggc gattttgtgg cagtggctac ggtggctgtc agcgccgcag
     9721 gttaacagga cgatactcat catttgcccc ggttattcgg ggcttttttg caggtggaga
     9781 atatgatgtt tctgaaacag ggcacgttta attatgaaaa acagtccgtg gtgctcagtg
     9841 agctgtccgg gctgcagaga attgaatatc tggcgtttgt tcagcagcga acggcaaagt
     9901 ttgatgccgg ggagggagaa ctgtcggagg ctgaacgaca gattgctttt ctgcggatgg
     9961 ggatggatat caatgcctgg ctggtttccc gatcactgtg gaatgcggat cagtctaagg
    10021 atgtagagac gctttgcgca tccgttatta caacatggtc gtatgatgcc ctgggggcgg
    10081 gggcggagat ggttctgtcg ctgagcggta tgggggccat tgataatgcc ggggatgatg
    10141 agcatgaggc gctgacgccg gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct
    10201 tgcccgggag ttccggcggg cagactggcg gcggatgctg tcggaaatgt cggccactga
    10261 gcttggtgag tggggcgatt atttccggat gcagagcttc agtgatgtgt ggatggatgc
    10321 gcagtttgcc tcgctgaagg cattgatcgt gagaatggtg tccggcagca gtgatgctgc
    10381 ggtggctgat ttcagccttt taccggaaga gaacgggata ccggagcgaa cggacgaaga
    10441 actgatgcat cttggagaag gtatttccgg aggtgtgcgt tatggaccag atagccaacc
    10501 tggtcattga tttggggatt gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa
    10561 accttctgaa tggtgccgcc agcgatgcag aacggtcttc tgcccgtatg cagcgtttta
    10621 tggagcgtca gactcaggcc gcccggcaga caacgcaggc ggcttcttcg gctgcaacag
    10681 ccgcctccgt ccatgcgcag acggtggaga agagcgcaca ggctcatgaa cgcatggccc
    10741 gcgaggtgga gaaaacccgc cagcgcatgg aggcactgag ccagaaaatg cgcgatgaac
    10801 aggcgcaggc catggctctg gcggaggctc aggataaagc ggctgctgcg ttttatcgtc
    10861 agattgacag tgtgaaacag gccagtgcgg ggctgcagga attacagcgt attcagcagc
    10921 agatccgaca ggccagaaac agtggcggga ttggtcagca ggattatctg gcgctgattt
    10981 ctgaggttac ggcgaaaacc cgtgttctta cacaggctga ggaagaggct acccgacaga
    11041 aagtggcgtt tatccgtcag cttaaagagc aggcaacccg ccagaatctt tcttcttctg
    11101 agttgcttcg tgccaaggct gcccagctgg gggtaagcag tgctgcagaa gtgtatatcc
    11161 gcaaaatgga gcaggcagga aaagccacgc attcgctggg tctgaaaagt gcagcggccc
    11221 gccaggagat aggcgttctg ataggtgaac tggctcgcgg caatttaggt gcgctgaggg
    11281 gatccgggat aacgctggct aaccgtgccg gatggataga cacactgatg tcaccgaaag
    11341 gcatgatgct gggcggggtt attggcggta ttgccgcggc cgtctatggt ctgggtaaag
    11401 cctggtatga cggtcagaag gagggggaag aatttaaccg ccagttgtcg ctgacggggc
    11461 attatgccgg agtcactgcc gggcagctgt ggacgcttag tcgtgctatt tccgggaatg
    11521 gtatcacgca acatgctgca gccggtgcgc tggctcaggt ggtggggagt ggtgcatttc
    11581 gtggaaacga tatcggtatg gtggcgagag ctgccgcaca gatggagcga tcggttggcc
    11641 agtcggtcag cgataccata aatcagttta agcggctgaa ggatgatcct gtaaatgccg
    11701 cgaaggctct ggacaatgag ctgcattttc ttactgccac tcagcttgag cagatacgtg
    11761 tccttgggga tcagggacgg tccagtgatg ctgcacggat agccatgtct gcactggcag
    11821 aggaaaccgg tcggcgtact gcggatattg ataataacct caatgcgctg ggcagtacgc
    11881 tgaagtatct gtctgattta tggagtcgtt tctgggatgc ggccatgaat attggtcgtg
    11941 aagactcgct ggatgaacag atttccgctt tacaggagaa agtgtcgcgg gcgaaaagac
    12001 tcccctggac ggcatcatct tctcaggttg aatacgatca gcagcgtctt aacgagcttc
    12061 aggagaaaaa acgccagaag gatttgcagg atgcaaaaga gcaggcagag cggaattatc
    12121 aggagcaaca gaaacgccgt aatgctgaaa atgctgcact gaaccggatg aatgaaacgg
    12181 aagcagcacg acatcagcgt gaaattgcgc gtattaatgc catgcagtac gccgatcagg
    12241 ctgtcaggga tgcggcgata caacgtgaaa atgaacgtta cgagaaagcc ctggcatccg
    12301 gtaagaaaaa aacacgcgaa acccgtaatg atgaggccac ccggttattg ctgcagtaca
    12361 gtcagcaaca ggcacaggtg gaaggacaga ttgctgctgc cagacagtca tcaggcattg
    12421 ccacggaaag gatgacagaa gctcataaac agcttctggc tctgcagcag cgcatcagcg
    12481 acctggacgg gaaaaaactg acggcagatg aaaagagtgt gctggcccgt aaagatgaac
    12541 tgattcaggc actgacgctg ctggatgtaa aacagcagga gcttcagaaa cagacggcac
    12601 tcaacgagct gaagaaaaaa acaattcagc tgaccagtca actggctgaa gaagagcgcg
    12661 ctcagcgtca gcaacatgac ctggatattg ccacggtggg tatgggtgat cagcagcggc
    12721 agcgatatca ggtacaactg agtcttcgcc agaaatacca gcaacagctg gagcagttga
    12781 ggcgggatag tgagcagaaa ggaacatata acacggatga ctacagaaag gccgagcagg
    12841 cgctgacgga gagcctgaac cgacaactga atgagaatcg ccgttactgg caacagcttg
    12901 aagttgtgca gggtaactgg aaaaacggag tcctgcgtgc atttcaggat tttaccgtgg
    12961 atgcagataa tacggcagga acagcagaac aggtgttctc gtcagccttc agctacatgg
    13021 gaaatggcct ggcaactttt gtcactaccg gcaaactcaa tttcaaatcc ttcacctctt
    13081 ctgtgctgtc agatatggcg aaaatcctgg cgcaggcaac catgatgaaa tcgataaaag
    13141 ggattggcag tgtactggga tttgatctca gcagcctttc cctgaatgcc aatgggggga
    13201 tttatcagtc tgctgatttg agtcgttaca gtggcacggt ggttaaccgt ccgacgtttt
    13261 ttgcttttgc aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgcctc
    13321 tgcgtcgtgg tgctgacgga aagctggggg ttgtggcgga tattggtggt tcaggtatgg
    13381 cgatgtttgc cccgcagtac aacatcgaga tcaataacga tggcacgaac gggcagatag
    13441 gtccggctgc cctgaaggtg gtttatgacc ttgggaaaaa agcggcagcg gactttatgc
    13501 aacagcaggc ccgtgatggt ggtcggttaa gtggagcata tcggtaatgg agacgtttca
    13561 ctggaaagtg cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct
    13621 gggcgatggt tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag
    13681 cgtgacgata cgtgttcgta aatgtgaaca cccatcttta aaagcctttc tggaacggca
    13741 cggtggcgtc cgcgcatttc agtggacgcc accttatgac tggaaaccga tcagggtggt
    13801 ttgtcgcaaa tggtcggcaa gcgtgggggc gctgtgggta accataacgg cagattttga
    13861 acaggtcgtg gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac
    13921 gacacgcctc actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg
    13981 tggtgaacgt tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca
    14041 ggggcggcag tatcaggcat accccattca ggggacggga tttgaactga atggcaaggg
    14101 cagtgctgcc cgtccgacac tgacggtttc taacctgcac ggcatggtca ccgggatggc
    14161 ggaagacctg cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt
    14221 tctggatgcg gtgaacttcg tcaacggaaa cagcgacgcc gatccggagc aggaggtgat
    14281 cagccgctgg cgcatcgagc agtgcagcga actgagtgcg gtcagtacct cctttgtact
    14341 gtccacaccg actgaaacgg atggctctgt ttttccgggg cgtatcatgc tggccaacac
    14401 ctgcacctgg acctatcgcg gcgatgagtg cggttatagc ggtccggcgg tcgcggatga
    14461 atatgaccag ccgacgtccg atatcacgaa ggataaatgc agcaaatgcc tgag
//
LOCUS       DAZGBC010000123        14468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGBC010000123 DAZGBC010000000
VERSION     DAZGBC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.124"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..125
                     /locus_tag="ACHK1J_003602"
     CDS             <1..125
                     /locus_tag="ACHK1J_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912823.1"
                     /translation="MTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPFE"
     gene            445..1833
                     /gene="narU"
                     /locus_tag="ACHK1J_003603"
     CDS             445..1833
                     /gene="narU"
                     /locus_tag="ACHK1J_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO7912824.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASITDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1928..5668
                     /gene="narZ"
                     /locus_tag="ACHK1J_003604"
     CDS             1928..5668
                     /gene="narZ"
                     /locus_tag="ACHK1J_003604"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO7912825.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGELRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     KELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5665..7209
                     /gene="narH"
                     /locus_tag="ACHK1J_003605"
     CDS             5665..7209
                     /gene="narH"
                     /locus_tag="ACHK1J_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7912826.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7209..7904
                     /gene="narW"
                     /locus_tag="ACHK1J_003606"
     CDS             7209..7904
                     /gene="narW"
                     /locus_tag="ACHK1J_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7912827.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            7901..8581
                     /gene="narI"
                     /locus_tag="ACHK1J_003607"
     CDS             7901..8581
                     /gene="narI"
                     /locus_tag="ACHK1J_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7912828.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8661..9554
                     /gene="yddE"
                     /locus_tag="ACHK1J_003608"
     CDS             8661..9554
                     /gene="yddE"
                     /locus_tag="ACHK1J_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO7912829.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDDYRISLEQGTPGFEPPLEDETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9650..10495)
                     /gene="nhoA"
                     /locus_tag="ACHK1J_003609"
     CDS             complement(9650..10495)
                     /gene="nhoA"
                     /locus_tag="ACHK1J_003609"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO7912830.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALRLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10668..11237
                     /gene="yddH"
                     /locus_tag="ACHK1J_003610"
     CDS             10668..11237
                     /gene="yddH"
                     /locus_tag="ACHK1J_003610"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO7912831.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEQYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11241..11468)
                     /gene="pptA"
                     /locus_tag="ACHK1J_003611"
     CDS             complement(11241..11468)
                     /gene="pptA"
                     /locus_tag="ACHK1J_003611"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO7912832.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(12063..12461)
                     /locus_tag="ACHK1J_003612"
     CDS             complement(12063..12461)
                     /locus_tag="ACHK1J_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912833.1"
                     /translation="MEMLGKILVIIISMIIISCDYYNAGMDYQDNIALEQVVTWDSDN
                     IFTPNIFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSI
                     TDKERENLKALICSKPAKSVNTDKRPALGY"
     gene            complement(12462..12902)
                     /locus_tag="ACHK1J_003613"
     CDS             complement(12462..12902)
                     /locus_tag="ACHK1J_003613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912834.1"
                     /translation="MIIGNGPVPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKI
                     QSRRDTWIWIIDTTDEEEQCMMNKAIDLNKKLKTINLGPLPVLNNCFSRTNMIFEACG
                     FNNPSMNTNAPISLQVLGELYGYQRYIMPKGPFTGFPFEFQEVK"
     gene            complement(12906..14321)
                     /locus_tag="ACHK1J_003614"
                     /pseudo
     CDS             complement(12906..14321)
                     /locus_tag="ACHK1J_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(14331..>14468)
                     /locus_tag="ACHK1J_003615"
     CDS             complement(14331..>14468)
                     /locus_tag="ACHK1J_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO7912835.1"
                     /translation="SGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGL
                     S"
BASE COUNT         3341 a         3836 c         3689 g         3602 t
ORIGIN      
        1 atatgacctt agatgaaata aacaatactt attgtaataa cgattattac aatcaggcaa
       61 taagagagga accgatagac cttctggaca gatcgttttc ctccagctca tggccttttg
      121 agtgaataaa ttattgctaa ttctataagt gtcttgtctt gttaagaata tagttaatat
      181 acaacccttt ggattcatca tctgaagggt tgaatcatga catgcgtaca tagctgaacc
      241 ccgtttaatg gttagcttgt tagttacatt tagtaacaca taactggctg cttaatcccc
      301 attctgcccc cgcggttacg ctttcttatc tattctttca atcagcactt cacccaattc
      361 atccttaatt ctaataactg gctattgcgc cttacctcct gttttgtctt ttttcattac
      421 caatgtgtgc atgtgaggaa caatatggca ctgcaaaatg agaaaaatag tcgttatctt
      481 ttacgcgact ggaaaccaga aaatccggcc ttctgggaaa ataaaggaaa acatattgct
      541 cgaagaaacc tctggatatc agtcagttgt ctacttcttg ccttctgtgt ctggatgcta
      601 tttagcgcag ttaccgttaa tctcaataaa atcggtttta attttactac cgatcaactc
      661 tttttattaa ccgcattacc ctccgtttct ggcgcattat tgcgtgttcc ctactccttt
      721 atggtaccta tattcggtgg acgccgatgg acggttttta gtactgcaat cctgattatt
      781 ccttgcgtct ggctcggaat tgccgtgcag aatccgaata ctccttttgg gatctttatc
      841 gttatcgctt tgctatgcgg ttttgcaggt gcgaactttg cttcgagcat gggcaatatc
      901 agtttcttct ttccaaaagc caggcaaggg agcgctcttg ggattaatgg cggattagga
      961 aacttaggtg taagtgtgat gcagctggtt gcaccgctgg tcatttttgt acccgtattc
     1021 gcctttctcg gcgtcaatgg cgtcccgcag gccgacggtt cggtgatgtc gctggcgaat
     1081 gccgcatgga tttgggtacc gctactggcg attgccacga tcgccgcctg gtcagggatg
     1141 aatgatatcg ccagttcacg cgcgtcaatt accgaccagc tccctgtctt acaacgcctg
     1201 catctctggc tgctgagcct gctttacctt gccaccttcg gttcgtttat cggtttttct
     1261 gcgggttttg ccatgctggc aaaaacccag ttcccggatg tgaatattct gcgcctggcg
     1321 ttctttggcc catttatcgg tgccatcgcg cggtcggttg gtggtgctat ttccgataaa
     1381 ttcggcggcg tgcgggtgac gttgatcaac tttattttta tggcgatttt cagcgccctg
     1441 ctgttcctta ccttaccggg cacaggctcc ggtaatttca tcgcatttta cgccgtattt
     1501 atggggctgt ttctgaccgc gggtctggga agtggttcta ctttccagat gatcgccgtc
     1561 atctttcgcc agataaccat ttatcgggtg aagatgaaag gcggtagtga tgagcaagct
     1621 cagagagaag ctatcaccga aacggcggcg gctctgggct ttatctcagc cattggcgca
     1681 gtgggcggct tttttattcc gcaggcattt ggcatgtcgc tcaatatgac cggctctcca
     1741 gtgggcgcga tgaaagtgtt tttaatcttc tacatcgttt gtgtgctact gacctggctg
     1801 gtttatggtc ggcggaaatt cagccaaaaa taagagatcg atgcctgaca cgtcttatca
     1861 ggctacaacc gtgtcgcatc cgacaaggca tcagatccca attcattcct ggagcaggag
     1921 tcatgtcatg agtaaacttt tggatcgctt tcgctacttc aaacaaaagg gcgatacctt
     1981 tgccgatggt cacggacagg tgatgcatag caaccgcgac tgggaggaca gctatcgcca
     2041 gcgttggcag ttcgacaaaa tcgtgcgttc cacccacggt gttaactgta caggctcctg
     2101 tagctggaaa atctacgtta aaaatggtct ggtgacctgg gaaatccaac agaccgacta
     2161 cccgcgcact cgccctgacc tgcccaatca tgaacctcgc ggctgcccgc gtggcgcaag
     2221 ttactcctgg tatctttaca gcgctaaccg cctgaaatac ccgctcattc gtaaacgact
     2281 gattgaactg tggcgcgaag ccctcaaaca acacagcgat ccggtacttg cgtgggcatc
     2341 gattatgaac gatccgcaaa agtgcctgag ctacaaacaa gtgcgtgggc gcggcgggtt
     2401 tatccgctcc aactggcagg aactaaacca gctgattgcc gccgctaacg tctggaccat
     2461 caaaacctac ggcccggatc gcgttgccgg tttctcgccg atcccggcga tgtcgatggt
     2521 ttcttacgcc gccggaacac gttatctgtc actattaggt ggcacctgtt taagcttcta
     2581 cgactggtat tgcgacctgc cgcccgcctc gccgatgacc tggggcgagc aaaccgacgt
     2641 gccggaatct gccgactggt ataactccag ttacatcatc gcctgggggt ctaacgtacc
     2701 gcagacacgt acgccggacg cccatttctt taccgaagta cgctacaaag gcactaaaac
     2761 catcgccatt acccctgact actctgaagt ggccaaattg tgcgaccagt ggctggcacc
     2821 aaaacaaggc actgatagcg ccctggcgat ggcaatgggc catgtgattt taaaagagtt
     2881 tcatctcgat aatcccagcg actactttat caactactgc cgccgctata gcgacatgcc
     2941 gatgctggta atgctggaac ctcgcgacga cggtagctac gtacccgggc gcatgatccg
     3001 cgcgtctgac ctggtagatg gactaggcga aagcaataat ccgcagtgga aaaccgtggc
     3061 ggttaatacc gcaggtgaat tggtagtgcc gaatggttcg attggtttcc gctggggaga
     3121 aaaaggcaaa tggaatctgg aatccattgc cgccggtaag gaaaccgaat tgtcgttaac
     3181 cctgctcggt caacatgacg ccgttgcagg cgtggctttc ccctactttg gcggcatcga
     3241 aaatccgcat tttcgcagcg taaaacacaa tcctgtcctg gtgcgtcaat tacccgttaa
     3301 aaacctgaca ttagccgatg gcagcacctg tccagtggtc agcgtttatg atttggtact
     3361 ggcgaattac ggcctcgatc gcgggctgga agatgaaaac agcgcgaaag attacgctga
     3421 aatcaaaccg tacacccctg cctggggcga gcaaattacc ggcgttccgc gccagtacat
     3481 tgaaaccatc gcccgcgaat ttgccgatac cgctcataaa acgcatgggc gctcgatgat
     3541 tatcctcggt gctggtgtca accactggta tcacatggac atgaactacc gcgggatgat
     3601 caacatgctg atcttctgcg gttgtgtcgg acaaagcggt ggcggctggg cacactatgt
     3661 cggtcaggaa aaactgcgcc cgcaaaccgg ctggttgccg ctggcctttg cgctcgactg
     3721 gaaccgacca ccacgccaga tgaacagcac ctcgtttttc tacaatcatt ccagccaatg
     3781 gcgctatgaa aaagtctctg cgcaggagtt actttcaccg cttgccgatg ccagtaagta
     3841 cagcggtcat ctgattgatt tcaacgttcg cgccgaacgc atgggctggc taccttctgc
     3901 gccgcagctg gggcgtaacc cgctcgggat taaagctgaa gccgacaagg ccggattatc
     3961 ccccacagaa tttaccgccc aggcgctgaa atcgggcgag ttgcgtatgg cctgcgaaca
     4021 accagatagc ggcagcaatc atccgcgtaa tttgtttgtc tggcgttcta acctgcttgg
     4081 ctcctccggc aaaggccacg agtatatgca gaagtatctg ctggggaccg aaagcgggat
     4141 tcagggcaag gaactcggtg ccagcgacgg gatcaaaccg gaagaagtcg agtggcaaac
     4201 tgcggcgatt gaaggcaagc tcgacctgct ggtgacgctc gacttccgca tgtccagtac
     4261 ctgcctgttc tccgatatcg ttctgcccac cgccacctgg tatgaaaaag acgatatgaa
     4321 cacctcggat atgcatccgt ttattcatcc gctatctgcg gcggtcgatc cggcctggga
     4381 atcacgcagc gactgggaaa tctacaaagg tattgccaaa gcattttcgc aagtgtgtgt
     4441 gggtcatctt ggcaaagaaa ccgacgtggt attgcaacca ctgctgcacg actctccggc
     4501 agagctctca cagccgtgtg aagtgctcga ctggcgcaaa ggcgaatgcg atctgattcc
     4561 aggtaaaacc gcgccgaata ttgtggcggt ggagcgcgac taccctgcta cgtatgaacg
     4621 ctttacctcg ctcgggccat tgatggacaa acttggcaac ggcggtaaag ggatttcgtg
     4681 gaatacccag gatgaaatcg atttcctcgg taaactcaat tacaccaaac gtgatggccc
     4741 ggcacagggg cgtccgctga ttgacaccgc cattgacgct tcagaagtga ttctggcact
     4801 ggcaccagaa accaacggtc atgttgcagt taaagcgtgg caggcgctgg gcgagatcac
     4861 cggacgcgaa catacccatc tggcgctgca caaagaggac gagaagattc gctttcgcga
     4921 tattcaggcg cagccgcgta aaattatctc cagccccaca tggtctggtc tggaaagcga
     4981 tcacgtctcc tataatgcgg gatacaccaa cgttcatgag ttaattccgt ggcgcacgct
     5041 gtcgggacgc cagcagctct atcaggatca tccgtggatg cgtgcttttg gtgaaagcct
     5101 ggtggcttat cgcccgccta tcgacacccg tagcgtcagt gagatgcgcc agatcccgcc
     5161 aaacggcttc ccggaaaaag cacttaactt cctgacgccg caccagaaat ggggcattca
     5221 ctcaacctac agtgaaaacc tgctaatgct gacgctctct cgcggtggac cgattgtctg
     5281 gatcagcgaa accgatgccc gtgaactaac cattgtcgat aacgactggg tggaagtatt
     5341 taacgccaac ggcgcgctga ctgcccgcgc ggtggtcagc caacgtgtac cgccgggcat
     5401 gaccatgatg tatcacgccc aggaacgcat tatgaatatt cctggttcgg aagtaactgg
     5461 catgcgcggc ggcattcata actcggttac ccgcgtttgc ccgaaaccaa cgcatatgat
     5521 tggcggttac gcgcagctgg cctggggctt taactactac ggcaccgtcg gctcgaaccg
     5581 cgacgaattc atcatgatcc gcaaaatgaa aaacgttaac tggctggatg atgaaggtcg
     5641 cgatcaggta caggaggcga aaaaatgaaa atccgttcgc aagtcggcat ggtgcttaat
     5701 ctcgataaat gtatcggctg ccatacctgt tcggtgacct gtaaaaacgt ctggaccggt
     5761 cgcgaaggta tggagtacgc atggtttaac aacgtcgaaa ccaaaccggg cattggttat
     5821 ccgaaaaact gggaagatca ggaagagtgg caaggcggct gggtccgcga tgtgaatggc
     5881 aagatacgcc cgcgtctggg cagcaagatg ggtgtaataa ccaaaatctt cgccaacccg
     5941 gtggtgccgc agattgatga ttactacgaa cctttcacat tcgactacga acatttgcat
     6001 agcgcaccgg aaggcaaaca tatccctact gctcgcccgc gttcgctgat tgacggtaag
     6061 cggatggaca aagtgatctg ggggccaaac tgggaagaac tgctgggcgg cgagtttgaa
     6121 aaacgtgccc gcgaccgcaa cttcgaggcc atgcaaaagg agatgtacgg gcagtttgaa
     6181 aacaccttca tgatgtacct gccgcgcctg tgcgaacact gcctcaatcc cagctgcgtg
     6241 gcgacctgcc caagcggcgc tatctacaaa cgcgaagaag acggcattgt gctgattgat
     6301 caggataaat gccgtggctg gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac
     6361 ttcaactgga aaagcggcaa gtcagaaaaa tgtatcttct gttacccacg aattgagtcc
     6421 ggtcaaccga ccgtgtgctc agaaacctgc gtgggtcgca tccgatatct gggcgtgctg
     6481 ctttacgacg ccgaccgcat tgaggaagcg gcaagcaccg agcgcgaggt tgacctctat
     6541 gaacgtcagt gcgaagtgtt cctcgatcca cacgatccct cagtgatcga agaagccctg
     6601 aaacaaggta ttccacaaaa cgtgattgac gctgcccagc gttcgcctgt ctacaaaatg
     6661 gcgatggact ggaaactggc gctaccgttg caccctgaat accgcaccct gccgatggtc
     6721 tggtacgttc ctccgctgtc accgattcag tcttacgctg atgcgggcgg tttgccgaaa
     6781 agcgaaggcg tgctccccgc catcgaaagc ctgcgtattc cggtgcaata tctcgccaat
     6841 atgttgagtg ccggtgatac cggtccggta ctgcgggcgc tgaaacggat gatggcgatg
     6901 cgtcactata tgcgttcaca aaccgtggaa ggcgttactg atactcgtgc catcgacgaa
     6961 gtaggcctga gcgtcgccca ggtcgaagag atgtatcgct atctcgccat tgccaactat
     7021 gaagatcgtt ttgttatccc gaccagccat cgggaaatgg cgggcgatgc cttcgcagaa
     7081 cgcaacggct gcggttttac ctttggcgac ggttgccacg gttcggacag taaattcaac
     7141 ctgttcaaca gtagccgtat cgatgccatc aacatcaccg aagtgcgcga caaagcggag
     7201 ggcgaataat gcagatcctc aaagtgatcg gtctgttgat ggagtatccg gacgagctgt
     7261 tgtgggaatg caaggaggac gcgctggcgt tgatccgccg cgacgcgccg atgcttacgg
     7321 atttcaccca caacctgctt aacgcgccgc tgctggataa acaggccgaa tggtgcgaag
     7381 tgtttgaccg cgggcgcact acgtcgctgc tgctgttcga acatgttcat gccgagtccc
     7441 gcgatcgcgg ccaggcaatg gtggacctgc tggcggagta tgaaaaggtc ggcctgcagc
     7501 tggattgtcg ggaactgccc gattatctac cgctgtatct ggagtattta agcatactgc
     7561 ctgacgatca ggcgaaagaa ggattgctca atgttgcgcc gatcctcgcc ctgcttggcg
     7621 gtcgcttaaa acaacgcgag gcaccgtggt acgcgttgtt tgatgctctg ctgcaactgg
     7681 cgggaagtac actttcaagt gacagtgtca caaaacaagt aaacagcgaa gagcgcgatg
     7741 acacccgcca ggcgcttgat gcagtgtggg aagaggaaca ggtgaagttt attgaagata
     7801 acgccacggc gtgtgacagc tcgccgctta atcaatatca gcgacgcttt agccaggatg
     7861 tcgcgccgca gtatgtcgac atcagtgcgg gaggtgagaa atgattcagt atctgaacgt
     7921 ctttttttac gatatctacc cgtacatttg tgcgacggtg tttttcctcg gcagctggct
     7981 gcgctacgac tacgggcagt acacctggcg cgcctcctca agtcagatgc tcgataaacg
     8041 cgggatggtg atatggtcga atctgttcca tatcggcatt ttggggatct tcttcgggca
     8101 cctgttcggc atgttaacgc cgcactggat gtacgcgtgg tttttgccag tggcagcgaa
     8161 acaactaatg gcgatggtgc tcggtggtat ttgcggtgtg ttgacgttga ttggcggcgc
     8221 aggactgctg tggcgcaggc tgacaaatca gcgcgtgcgt gccacttcca ctacgccgga
     8281 tatcatcatc atgagcattt tgctggtcca gtgtctgttg gggctaagca ccataccgtt
     8341 ttcagcacag tatcctgatg gtagcgaaat gatgaagctg gtgggttggg cgcaaagcat
     8401 tgtcactttc cgtggtggat cgtcagagat gcttaacggt gtagcgttcg ttttccgcct
     8461 gcatctggtg ttgggaatga ccatcttcct gctcttcccg ttcacccgac tggtgcatgt
     8521 gtggagcgcg ccgtttgagt acttcacccg ccggtatcag attgtgcgct cgcggcgata
     8581 attttcaccc tggccggata gcccttcgct ctccggccac ttgcaggcta tgatagttct
     8641 ccagccacag gagaacgtta atgaaaccgc aggtttacca cgtcgatgct tttacctctc
     8701 aaccatttcg cggcaattct gccggtgttg ttttccccgc cgataatctt agcgaagcgc
     8761 aaatgcagct tatcgcccgc gaattaggtc attcggaaac tgcttttctg ctgcacagcg
     8821 acgacagcga tgtgcgcatc cgctacttta cgccaacggt tgaagttccg atttgtggtc
     8881 acgcgacagt agctgcgcac tatgtacgtg cgaaggtgtt aggtttagga aattgcacgg
     8941 tctggcaaac atcgctggca ggaaaacatc gcgtgactat cgaaaagcac cacgatgatt
     9001 atcgtatttc gctggaacaa ggtacgccgg gctttgagcc accgctggaa gatgaaacac
     9061 gtgcggcgat tatcaacgcg cttcatctca ctgaggacga tattctgcca ggcttgccaa
     9121 ttcaggtggc aacaacagga cactcaaaag tgatgatccc gttgaaacca gaagtggata
     9181 tcgacgccct ttcgcctgat cttgctgcgc tgagcgctat cagcaaacag attggctgca
     9241 atggtttctt cccgttccag atccgcccag gcaaaaacga aaccgatggt cgcatgttct
     9301 cgcctgcgat tggtattgtg gaagatccgg tgactggcaa tgccaatggc ccgatgggtg
     9361 catggttggt acatcacaac gtattgcccc acgatggcaa ggtgctgcgt gttaaaggcc
     9421 atcaggggcg cgcattgggg cgtgacggcg tgattgaagt gacggtgaca attcgtgata
     9481 accaaccgga aaaagtcacc atttctggcg cggcggtgat tttgtttcat gccgaatggg
     9541 caattaaact ctgaaaccgt tgcctgatgc gcttcgctta tcaggtctac aaccgagcta
     9601 atgtatgccg gataaggcgt ttacgccgca tccggcaacc tgacataaat tattttcccg
     9661 cctccgggtg cgtatcaaac gccgccatca ccagcgctaa ctcatccacg gtaaagccat
     9721 gtttcgcatc atccacgccc agaccaaact gttcttgcat cacagcatat aatgacgcca
     9781 catccggtag atttcgctgc tccaccgcgt gcccattttc ataatgggta aaatgaaaat
     9841 tggtcagtgt cagcttgccg ccgtccggca aatggcggca catcagcaaa tgatggcgaa
     9901 aatgcgactg cggccagtgc gccgaccaga aattgcccat cacataatcg ctttgttgct
     9961 gctcgcagag atcaaaacgg tacatcgact gccaatgctg atgatgatta aactgcaaca
    10021 cccaatcatc accctcctgc aacaaccgat actctccgtg tggcgtggtc tgcacgagat
    10081 cgggaactaa acgaatcggc gcggttagcg tctgcccacc gaaaccgaca tcagcaatcc
    10141 atttttcccc ttccagttca accaacagca aacgatgggt gcgcggcggt aatgctggcg
    10201 gatttgataa cactacccgc cctaacaagc tgcgaacgtt aaaccccagc tcgcgtaaca
    10261 cccgctcaaa cacgccattc tgctcaaaac agtaaccgcc acgacgggct atcaccagtt
    10321 tctcttccag cgattgatca tcaagctgta tttccctcgg cagcaaaacg tcgaggtttt
    10381 caaacggaat ggtgcaattg tgtttcaggc gcaatgcacg aagcgtatca atattgaccg
    10441 cagcagctcc cgaccagtta atacgggcaa aatagtgatt cagaatgggc gtcatcaggc
    10501 gtttccctta gtggttttct caacttataa cctggaatag acgccgcacc ggctattttg
    10561 tgcgatcgcg gacatgcatc acgttcgcaa atcgccaatc ccgttaattt cgttacttat
    10621 tgcgttacgg actacgctta aaaaaacctt cttcgcgagg cgataccatg agccgattca
    10681 tccccataga attacaccat gccagccgtc tgttaaatca tggcccaact gtcttgatca
    10741 ccagtttcga tgagcaatcc cagcggcgta acattatggc tgcagcctgg tcaatgccgg
    10801 tggagtttga acctccacgc gtggcgattg tggtagataa atcgacatgg accagggagt
    10861 tgattgaaca taacggtaaa tttggcatcg ttatcccggg cgttgcagca actaactgga
    10921 cgtgggcagt gggaagtgtg tcggggcgtg aagaagataa atttaattgc tatggcattc
    10981 cggttgtgag aggcccggta tttggtttgc ctctggtcga ggaaaaatgt ctggcgtgga
    11041 tggagtgtcg cctgctacct gcgacttctg cgcaagaaca atacgacaca ctgtttggcg
    11101 aggtagtatc agcagcggca gacgcacggg tatttgtcga aggccgctgg cagtttgatg
    11161 atgataagct caatacgttg catcatttag gtgctggcac gtttgttact agcggcaagc
    11221 gtgttacggc gggttaagca ttacatgctg taaccaggtt tctttatcaa agcctccatt
    11281 tggggcgcga tttcggcatc ccagatagct tgccatgatt ctggttgaat ctgctgtaga
    11341 gaaatgctta tcgagctatc tttactgttc agatggcgaa taataacgtc ggtaatatct
    11401 gcagcaagtg ctgctttttg ttgttcgtcc agttcacgcg gaaaacattt aatgtcgatg
    11461 tgcggcatga ttgtgctcct tttgaaagat gtcagtaaaa ttaacacaca ttgtgaatgt
    11521 cgtatgtccg gtgatgcgtt tttacgacaa taatgcctac ttgaaattgt agatatttgc
    11581 actacaccct tttaaataaa gcaccaacaa atcgtcaact tgcagaacat attcttctgc
    11641 ctacatacca ttaagaaaac atcaagttcc cgacttgcca atttattaac gcccattata
    11701 gacgctgaaa caagctacgg acaaaggaga tgccaaaatt accggcaatg cagaaaaact
    11761 caacgaacca tggctcttta ccacagcagc cggggttgtt tgggaccgac ttccgacagc
    11821 agatcccggt gggcctgcca ccatctcttc acaagcttat gcttattaca tgcaaaacgt
    11881 cccgttttgc atctttgtgc ctacggcaca cagtgatctt tccctggtcc ctgtcccgga
    11941 ttgtagtacc ggtgccagtt gtagtgcagt tcggattcgt cgtcatactg ctagcccgga
    12001 tggcggagca gttgctctag tcactcagaa tattgtaata gtgatagagt gcagtgccgg
    12061 tttcaatatc ctaacgctgg tcttttgtct gtattaactg atttagctgg tttgctacat
    12121 attaatgcct tcagattttc cctttccttg tctgtgatac tgtcgcaaac cctactagcc
    12181 cattctactt cactcttctc caggcattct attttcttat tgctcattgt tctaatgttt
    12241 ggtgttgcat ctccatttaa taacatccat gtttcatttt tcttcataaa acatcttcca
    12301 ttatgttcaa agatattagg tgtgaatata ttatcagaat cccatgttac gacttgctcg
    12361 agggctatat tatcttgata gtccatgcca gcattataat aatcacaaga tataattatc
    12421 atggaaataa ttatcactaa tatcttccca agcatttcca tttactttac ctcttgaaat
    12481 tcaaatggga atccagtgaa aggaccttta ggcataatat atctttgata tccataaagc
    12541 tcaccgagca cttgtaggct tataggcgca tttgtattca tagaggggtt gttaaacccg
    12601 cacgcctcaa aaatcatgtt cgttcggcta aaacaattat ttaaaacagg aagaggcccg
    12661 aggttaatag tttttagttt tttattaagg tctattgcct tattcatcat acattgttct
    12721 tcttcatctg ttgtatctat tatccaaatc caggtatctc tgcgggattg tatcttttta
    12781 aaataatcag agacagaggc tcccatctca tccccagttc ccgaactcat taatccatat
    12841 cgatttgccg ccgcagcatg cccaaaagga ttatcaggaa cggggccgtt acctattatc
    12901 atagtactaa gccctaatgg atcaacgtgt tgcactggat tcagcggata ttggtacaga
    12961 ttccatcccc ccttcagccc aatcgggtcc tggatgatat accgcccctg caacggatcg
    13021 tagtaccggt gacggttata atacagcccc gactcctcat cgtgttgctg ccctggcaga
    13081 cggtacggct ggtgcaggtg atgcgggttt tcctcattca gcaggtttcc ccactcatca
    13141 tactccccgc ttcacgcagt attgccgtct tcgctgatga gcgccagcgg caggccccgg
    13201 tggtcgcagt ggtaaaaatg aacttttcgc gccggtgtgt attccggctc cacctgtctg
    13261 gccagttgct ccaccgtcag cccgcactgc gcaagccacg cccggctttc actgctcacg
    13321 cggtctgccc ggatttcttc ctccagtctg tccagcagcc gcaccagttc agccggaaat
    13381 accacgccgt gcccgtcctc gctcccttcc tgctgcatct tctccgccag gctgcggcgc
    13441 tgcgctttct cccgctcgcc gttctctgtc tcgacccgga tgagcggcgt gaagcttccc
    13501 ggctcgtata ccgtctggat acgtgtggtg tcagtctgca ccgtcgtcag cctgtcgccg
    13561 tcccacccgt accatgtctc ctccggttta cgcgacagcg acatccatcc cgtcaggtca
    13621 cgcccgcgac gccacacccg tttcgccatt cgccgtccca gcgggtcgta gaggtagcgg
    13681 ctctcgacca gtggctcgcc atgctgtatc cgcgtgtaga acaccagccg gtgctggctg
    13741 tcgtagtgat agtggtgggt gcgctcgtcg tgcatccgga taaccccttc cgggatacgg
    13801 tccgtcttct ccgccagcct gccgtattca tcgtagcgat agacatagtg cgcatcttcc
    13861 gcgatgcggt tatccggcca cgccgtgaag gtactgtccg ggtgcagttc cgggtccggc
    13921 agccggtttc ccgccgggtc tgttgcatac gggatatcga tatccaggtt cgccgccgtg
    13981 gtgtggacgc ccgtcagcct tcccgtgtca ctgtaacggt actcccggct ctcctgcggg
    14041 ccgctgatgc ggattagctg tccgttgtcg ttccagtcgt agtcacggtc aagctgcgga
    14101 aggttcaggt gcctgctctg gagctggccg ctggtattcc aggcggtggc aagttcatat
    14161 gcctccccgc cgaagctgcg ggccgtctca cggtgcagcc ggtcccgcgt gtactcgacc
    14221 agcggcgttc cgcccagctt catccccgca agataaccgc tgccatacgt cagccactcc
    14281 accggcggca gaccgtccgg ctcctgacgg gtcgccagcc cagggcaaga ttacgaaagc
    14341 ccgctccccg caaggactga cgccagatag tttctgtcca tggctgcttt tcgcatctta
    14401 cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa tattaatagc aatgtgccgt
    14461 atccctga
//
LOCUS       DAZGBC010000124        14381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGBC010000124 DAZGBC010000000
VERSION     DAZGBC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.125"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..302)
                     /locus_tag="ACHK1J_003616"
     CDS             complement(<1..302)
                     /locus_tag="ACHK1J_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7912836.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVKRPVAITIVVVVMLITLTALVGVLAASFNEFISMLPKFNKEL"
     gene            434..1651
                     /locus_tag="ACHK1J_003617"
     CDS             434..1651
                     /locus_tag="ACHK1J_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7912837.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMAVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(1655..2212)
                     /gene="dcrB"
                     /locus_tag="ACHK1J_003618"
     CDS             complement(1655..2212)
                     /gene="dcrB"
                     /locus_tag="ACHK1J_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO7912838.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(2285..2950)
                     /locus_tag="ACHK1J_003619"
     CDS             complement(2285..2950)
                     /locus_tag="ACHK1J_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO7912839.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLTDKSEINAL
                     QAS"
     gene            3171..3416
                     /gene="tusA"
                     /locus_tag="ACHK1J_003620"
     CDS             3171..3416
                     /gene="tusA"
                     /locus_tag="ACHK1J_003620"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO7912840.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(3518..5716)
                     /gene="zntA"
                     /locus_tag="ACHK1J_003621"
     CDS             complement(3518..5716)
                     /gene="zntA"
                     /locus_tag="ACHK1J_003621"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO7912841.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(5790..6416)
                     /locus_tag="ACHK1J_003622"
     CDS             complement(5790..6416)
                     /locus_tag="ACHK1J_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO7912842.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISSFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            6557..6916
                     /locus_tag="ACHK1J_003623"
     CDS             6557..6916
                     /locus_tag="ACHK1J_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO7912843.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(6919..7188)
                     /locus_tag="ACHK1J_003624"
     CDS             complement(6919..7188)
                     /locus_tag="ACHK1J_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO7912844.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(7178..7774)
                     /gene="rsmD"
                     /locus_tag="ACHK1J_003625"
     CDS             complement(7178..7774)
                     /gene="rsmD"
                     /locus_tag="ACHK1J_003625"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO7912845.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAKCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLYREKVAGQVAYRLYQREAQGESDAD"
     gene            7924..9420
                     /gene="ftsY"
                     /locus_tag="ACHK1J_003626"
     CDS             7924..9420
                     /gene="ftsY"
                     /locus_tag="ACHK1J_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO7912846.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKDEM
                     GEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            9423..10091
                     /gene="ftsE"
                     /locus_tag="ACHK1J_003627"
     CDS             9423..10091
                     /gene="ftsE"
                     /locus_tag="ACHK1J_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO7912847.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            10084..11142
                     /gene="ftsX"
                     /locus_tag="ACHK1J_003628"
     CDS             10084..11142
                     /gene="ftsX"
                     /locus_tag="ACHK1J_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO7912848.1"
                     /translation="MNKRDAINHIRRFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            11387..12241
                     /gene="rpoH"
                     /locus_tag="ACHK1J_003629"
     CDS             11387..12241
                     /gene="rpoH"
                     /locus_tag="ACHK1J_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO7912849.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            12512..13615
                     /gene="livJ"
                     /locus_tag="ACHK1J_003630"
     CDS             12512..13615
                     /gene="livJ"
                     /locus_tag="ACHK1J_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO7912850.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(13815..14198)
                     /gene="panM"
                     /locus_tag="ACHK1J_003631"
     CDS             complement(13815..14198)
                     /gene="panM"
                     /locus_tag="ACHK1J_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO7912851.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         3449 a         3685 c         3960 g         3287 t
ORIGIN      
        1 agctccttat taaacttcgg cagcatagag ataaattcgt taaacgatgc cgccagtacg
       61 ccgaccagcg cggttagtgt gatcagcatc accaccacca caatcgtaat ggcaacgggg
      121 cgttttactc cccgtcgaat aaaccaggta acgagcgggt tgaggacgat ggcaaaaaac
      181 agcgccagta acagctgcac aatgatatct gccgctgcgt gaatgcccgc gaggatcact
      241 accagcgagg ccagcttgag cagaatgtgc atgcccgttt tatcgggttg aggggtttcc
      301 attggggctt ccttgtgact ttttgtatta agtgtagcgg gagtcacgcc agcaatattc
      361 tgattcttac tgctgatttt cacgtcagca cgtggtaaaa atgaaacact gttgtaaaaa
      421 tgtggtgatc ctcatgcccg aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa
      481 tttgcgcatt gtctccattg tcatgtttaa cttcgccagc tacctcacca tcgggttgcc
      541 gctcgctgta ttaccgggct atgtccatga tgtgatgggc tttagcgctt tctgggcagg
      601 attggttatc agcctgcaat attttgccac cttgctgagc cgtcctcatg ccggacgtta
      661 cgccgatttg ctgggcccca aaaagattgt cgtcttcggt ttatgcggct gctttttgag
      721 cggtctggga tatctgacgg caggattaac cgccagtctg cccgtcatca gcctgttatt
      781 actttgcctg ggacgcgtca tccttgggat tgggcaaagt tttgccggaa cgggatcgac
      841 cctgtggggc gttggcgtgg ttggctcgct gcatatcgga cgggtgattt cgtggaacgg
      901 tattgtcact tatggggcga tggcgatggg tgcgccgtta ggtgtcgtgt tttatcactg
      961 gggcggcttg caggcgttag cgttaatcat tatggccgtg gcgctggtgg ccattttgtt
     1021 ggcgatcccg cgtccgacgg taaaagccag taaaggcaaa ccgctgccgt ttcgcgcggt
     1081 gcttgggcgc gtctggctgt acggtatggc gctggcgttg gcttccgccg gatttggcgt
     1141 catcgccacc tttatcacgc tgttttatga cgctaaaggt tgggacggtg cggctttcgc
     1201 gctgacgctg tttagctgtg cgtttgtcgg tacgcgtttg ttattcccta acggcattaa
     1261 ccgtatcggc ggcttaaacg tagcgatgat ttgctttagc gttgagataa tcggcctgct
     1321 actggttggc gtggcgacta tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc
     1381 cgggttttcg ctggtgttcc cggcattggg tgtagtggcg gtaaaagcgg ttccgcagca
     1441 aaatcagggg gcggcgctgg caacttacac cgtatttatg gatttatcgc ttggcgtgac
     1501 tggaccactg gctgggctgg tgatgagctg ggcgggcgta ccggtgattt atctggcggc
     1561 ggcgggactg gtcgcaatcg cgttattact gacgtggcga ttaaaaaaac ggcctccgga
     1621 acacgtccct gaggccgcct catcatctta aaacttactg aataaccagc gtattaatga
     1681 tgttttctgc ggtggtctgc gctttttgct gatcgtcagc tggcagagta atttgcatgg
     1741 tcagcagttg attacccacg ttacccagaa taacggaaga gtacgccgtc tggcctttcg
     1801 cggagataat gctgtctaac tgctgcattt tgtgaccttt cagctcaatg gctttgttgg
     1861 tcactacttg cagctgcgga tcgcggctac gttgctgatc ttccagacgc ttcgccagca
     1921 ccgccagatc ttctttcgga tcatcgccca tgatgacgat gactgctttc tgcccggtgg
     1981 cgtcggacca gacatgcatg ttattggcct gcgttcccag cttaccgctc tggtcggtca
     2041 tatccgctgg cagcgagaaa cttaacttgc catcaagcag gttgacggga ttcccggtag
     2101 cgttactttc cgcgaccgaa ccctgcgccg tagcgttagt gtctttatca tcacaggccg
     2161 caagccccat aaccagcagg ccaattccga catatttaac cagattgcgc attgacttct
     2221 tcctttcgat aaacggccat aacggctcat tcatccatct tatcacaact ctgataacga
     2281 acctttaact cgcctgcaaa gcgttgattt cggatttatc tgtcagtctt ttcaacagca
     2341 tattgagtaa tacgccatac attggcagga agaaaacgat actgattaac actttgaaac
     2401 agtaatcgac cagcgcgatt tccatccagt gttcagccat aaaggcatcc gggctacgcc
     2461 agaaggcaat gaagaaaaag gccagcgtgt cgctgacgtt accgaacagt gtggacgctg
     2521 tcggtgccag ccaccagcgg cgactctgac gcaggcggtt aaaaacgtgc acgtcgagga
     2581 tctgtcccag cgcgtaggcc atgaaactgg cggtggcgat acgggcgaca aacaggttga
     2641 agtgggcgag tgcgccgaat ccctgccagg aacccatata gaatagcgac gagatgacgt
     2701 aggagatcag caatgccggg atcatcaccg caaagatgat acgacgggcc agcggtgcgc
     2761 caaaaatacg tacggtcagg tcggtagcaa ggaaaataaa cgggaagcta aacgcgcccc
     2821 aggtggtatg gaaaccaaaa atggagacgg gaagctgaac cagatagtta ctggaggtga
     2881 tcaccagcag atgaaataac gataaccaga acaacgcctt atagcgttga gtttgcgaga
     2941 aaacgttcat attgtacctt tttgattaac cattggggtg agggaaccca atacgtacga
     3001 cacgtctgtt accttaacat aataacgtgc ttaccctttt ttcgagccgc cgcatgatac
     3061 tgctttacgt tgacaatgca atggttaatt ttcacgcaat cgttaacctg gtttgcttac
     3121 ggactcacag ggcgtaaact agcgccgttt ttttatgtga tgagaagaaa atgaccgatc
     3181 tcttttccag ccctgaccac acactcgacg cacttggcct gcgctgcccg gaacccgtaa
     3241 tgatggtgcg caaaacagtg cgcaatatgc agcctggtga aacgttgctg attattgctg
     3301 acgatccggc gacgacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg
     3361 ttgctaaaga gacggatgga ctgccttatc gttatttgat tcgtaaaggc ggttgatggg
     3421 ggcagattgg cttcgatgcc gccttttccc ctcaccctaa ccctctcccc agaggagcga
     3481 ggggaccgat cgcgctcaat gttgcgatcg gtttgcctta tctcctgcgc aacaatctta
     3541 acgcattcgc tgtcaccagc accgtcgcac ccgtatctgc cagcaccgcc agccatagtc
     3601 cggtcattcc taacagcgtg gtgacgagga agatcccttt cagccccagc gcaatagtga
     3661 tgttctggcg gatattggcg tgagtggcgc gtgccagttc aatcatttgc accagaccgc
     3721 gcaggtggtt atgggttaat gctgcgtcgg cggtttccag cgccacgtct gtgccgctgc
     3781 ccattgcaat cccgatggcg gcagctttca tcgctggcgc gtcgttaata ccgtcaccga
     3841 ccatcgccag cggcgcatgt tgattcagct cggttaccgc tttgacttta tcttccggca
     3901 acaggcccgc tttaaactcc agccccagct ccccggcaat tgccgccgct gcgcgtggat
     3961 tatcgccggt gaggatcacc cctttgacgc ccagcgcgtt cagttcactg atggcagttg
     4021 cagcatcggc gcgcagggta tcctgtaacg caatgacacc cagcacgtca tcgttacgta
     4081 ctaccagcac taccgtttgc ccggcgcttt ccagttcgtt aattaaacca gcaaatgcat
     4141 cagcgggatg tttcccggca gcgcaaatca atacgcgctc accgttaacc tgcgcttcaa
     4201 tgccagaccc gaccagcgcc cgctgtgatt cggcggcggg aatggcgagt gcagcaacct
     4261 gtgcttcgcg cacgatggcc tgcgccagtg gatgcgtcgc gccttgctcg accgccgccg
     4321 ccagtgtcag cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg
     4381 gtttaccgac ggtcagcgta ccggttttat caaacgccac ctgggtaaca cgacctagct
     4441 gctccagcgc cgcaccgcct ttaattaacg ccccacgacg tgccgctgct gccagcccgg
     4501 aggtaatcgc cgcgggcgtg gagataacta acgcacacgg gcagccaatc agcagcagcg
     4561 tcaggccttt ataaatccac tcttgccagc tggcggcaaa cagcagcggc ggcaccagcg
     4621 ttaccagcag ggcgacggcc ataatcgctg gcgtataaat acggctgaaa cggtcgataa
     4681 accgctcaat gggagcgcga cgctcttcgg cttcttcgat cagtttcaga atccggtcaa
     4741 tggcgctggc tcccggttcg gacaatactt ccagcgttac cagacggtct acgctggtgg
     4801 ctcctgcagg aactttatcg cccgttgcgc gctccaccgg aatggattcg ccggtcaggg
     4861 cgctttcatc aaaactggca aagggcgaga gcagtttacc gtcggcaggc aaacgcccgc
     4921 ccgccgcaac ttcaatcaca tcgccagggc gcaggctgtt aatcgccacc tcttcccgct
     4981 caccgttacg caggcgcgtg gcggtttctg gtttcagcgc catcaacgcg ctaacgccct
     5041 gacgcgcgcg gctggcggcc cagccttcca gtcgttcacc aatcaaaaac agcagcaaca
     5101 ccatcgcagc ttcagccgtt gcgccaataa acagcgcacc aatagcagct acgctcatta
     5161 acgtttcaat ggcgaagtaa ctgcctgatt tgatcagccg taatgcctga cgagcaatcg
     5221 ggtacagccc aaccagcgtg gtcgcgataa acgccagttg accgaacgga tgattaaatt
     5281 gctccagtcc ccagctgatt gccatcatca cgataagcgt aatcagcggc agattctctt
     5341 tcaggcgcga ttcttgtggc tcgtcggcgg cctgctcatc gcgcagggaa tagcccgctt
     5401 tttgcaccgc agattcaacc tgggcacgga tgtcattgtc ggcatcaacc accagttttt
     5461 cggtggcgaa cagtacctgc acctgattca cgcctgcaag ctggcgcacg gcattttcta
     5521 ccttgcgcgc acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg
     5581 agacgttttc agagagcgtt ggcgtgctgg aacatgcgcc gtcgcagcaa cagtcgttgg
     5641 cgttctgtac cgtggttagc ggtttgaacg cagcaaattg aggggctttc ttgccgtgat
     5701 tgtcaggagt cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac
     5761 tctggagtcg actccagagt caagttttat cagagataca gcgagcggac gatgaggaag
     5821 tgaccggcaa agtagcaggc cgcagcaatc gcgttatccg cacggaagcg tcggcgatag
     5881 tggctaccca gccagacaaa gctactgata aacagcaacg aagcgccgac aaacgcagag
     5941 agtgccggag cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc
     6001 gtcatgccga taaacgtgca gataggccaa cgatactctt ccagccgcgt ccagataatc
     6061 gccagtaaca gcgcgcccag aaccagcagt accaacggca gaggccagaa gaaagagagc
     6121 gtcatctgac tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg
     6181 gcgtacatca gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac
     6241 agccctgcca gcaccagata gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac
     6301 agcaggagaa gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac
     6361 gatgcatcca cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta
     6421 gttccttgaa ttcgttattt tttcgtaggc ggaatgccta cggtcttgct tccagtttag
     6481 tggcgagggt ggacggatga caacaccata actggcaggg tatgcttaat tccgcatttt
     6541 ccgatgaggg ataaagatga gcaaaccacc tcttttcttt attgttatca ttggcttaat
     6601 catcgtcgcc gcatcgtttc gttttatgca acagcgacgg gaaaaagctg ataatgatat
     6661 ggcaccgctc cagcaaaagc tggtggtggt gagcaacaag cgggaaaaac cgattaacga
     6721 tcgccgttcg cgtcagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag
     6781 cttcaaaccg caaagcggag gaatggagca gacgtttcgc ctcgacgccc agcagtacca
     6841 cgccctgaca gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgttagctt
     6901 tgttggcgaa caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc
     6961 aaacacgccg aaaaggaaaa ttcgcacctt ttcggcggtg gtcatctgcg ggccatcttt
     7021 cggtaaagtg gatttcaata gcgccagctg cataccatgc atcagcacgg taaaaatcag
     7081 cgccacgtta acgaaaatat tcagcgggcg cgggaaggga tgcaccaggt tgaggattaa
     7141 aaatccccaa acgcagagca ttaacaaacg tccaatatta atcagcatca ctttctcctt
     7201 gtgcttcgcg ttgatacagc cgataagcca cctgacccgc cactttttcc cgatacaatg
     7261 accagtttgc cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg
     7321 cttcgtcagc cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac
     7381 ggcggaacgg tggatcgaca aacacgatat tatgcggtgt gcctttttgc gccaggaatg
     7441 acatcgcgtt gctgttcact acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa
     7501 ttaactgctg agaaactgca cgatccatct caatcaacgt ggcccccgca gcgtagcgtg
     7561 ataacgcttc cagccccagc gcgccgcttc cggcgaagca atccagacat ttggcgtcaa
     7621 caatgaccgg agccagccag ttaaataacg tttcgcgtac gcggtcggtg gtggggcgca
     7681 gacctgggct atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga
     7741 tttggccgct gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa
     7801 cacatacttg caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt
     7861 tagctgctat gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg
     7921 caaatggcga aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag
     7981 cagaccccgg aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt
     8041 caggcgcaag agcctgtgaa ggcttcggaa caagccgttg aagagcagcc gcaggcgcat
     8101 actgaagccg aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggtagct
     8161 gaaagtgaaa aagcgcagcc tgaagcggaa gtcgttgcac agccggaacc ggtcgtagaa
     8221 gaaacgccgg agccagtggc tatcgaacgt gaagaactgc cgttgccgga agacgtcaac
     8281 gccgaagagg tttcgccaga agagtggcag gctgaagcgg aaaccgtaga gattgtcgaa
     8341 gcggcggaag aagaagcggc taaagaagaa attaccgacg aagagctgga agcacaggcg
     8401 ctggctgccg aagcggcaga agaggcggtg atggtggttc ctccggcaga agaagagcag
     8461 ccggtggaag aaatcgctca ggagcaggaa aaaccgacca aagaaggttt tttcgcgcgc
     8521 ctgaaacgca gcctgttaaa aaccaaagaa aatctcggtt ccggatttat cagcctgttc
     8581 cgcggtaaaa aaatcgacga tgatctgttt gaggagctgg aagagcagct tttgatcgcc
     8641 gatgtgggtg tggaaaccac acgtaaaatt atcaccaatc tgacggaagg cgcatcccgc
     8701 aagcagcttc gtgatgccga ggcgctctat ggcctgctga aagacgagat gggcgagatt
     8761 ctggcgaaag tcgatgagcc gctgaatgtt gaaggcaaag cgccgtttgt gatcctgatg
     8821 gtgggcgtca acggtgtggg taaaaccacg acgattggta agctggcacg tcagtttgag
     8881 cagcagggta aatcggtgat gctggcggcg ggcgatactt tccgtgcagc agcggttgaa
     8941 cagcttcagg tctggggtca gcgcaacaat attccggtga ttgcccagca taccggtgcg
     9001 gattccgcct ctgttatctt cgacgccatt caggcagcta aagcgcgtaa tatcgacgtc
     9061 ctgattgccg ataccgccgg acgtctgcag aacaaatcgc atctgatgga agagttgaag
     9121 aaaatcgtcc gcgtgatgaa gaaactcgac gttgaagcgc cgcatgaagt tatgctgact
     9181 attgatgcca gcaccgggca gaacgcggta agccaggcca aactgttcca cgaagctgtt
     9241 ggcttaaccg gcatcacgct aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc
     9301 tcggtggctg accagtttgg tatccctatc cgctacattg gtgtcggcga acgtattgag
     9361 gatttgcgtc cgtttaaggc ggacgacttt atagaggcac tttttgcccg agaggattaa
     9421 caatgattcg ctttgaacat gtcagcaagg cttatctcgg tgggagacag gcgctgcagg
     9481 gcgttacgtt ccatatgcag ccgggtgaga tggcgtttct gaccggtcat tccggcgcag
     9541 ggaaaagtac cctcctgaag ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct
     9601 ggtttagcgg ccatgacatc acgcgtctga aaaaccgtga agttccgttt ctgcgccgcc
     9661 agattggcat gattttccag gatcaccatc tactgatgga ccgtactgtc tacgataacg
     9721 tggcgatccc gctgattatc gccggtgcca gcggtgacga tattcgtcgc cgggtgtcgg
     9781 cggcactgga taaagtcggg ctactggaca aagcgaagaa cttccctatt cagctttctg
     9841 gcggtgaaca acagcgtgtt ggcattgccc gcgcggtggt gaataagccc gcggtactgc
     9901 tggcggacga accgactggt aacctggacg acgcgttgtc ggaagggatt ctacgtctgt
     9961 ttgaagagtt taaccgcgtt ggggtaaccg tattgatggc aacgcacgac atcaacctga
    10021 tttcgcggcg ttcctatcgc atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg
    10081 gccatgaata agcgcgatgc aatcaatcat attcggcggt ttggcgggcg tctggatcgc
    10141 ttccgtaaat cggtcggcgg ctcaggcgac ggcggtcgta acgcgccgaa acgcgcgaaa
    10201 tcctcgccaa aaccggtaaa tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc
    10261 ttccacggcg cattgcagga tctgaaaagc aaaccgttcg ccacgttttt aacggtgatg
    10321 gttatcgcca tctctctgac gctgcccagc gtctgttata tggtgtacaa aaacgttaac
    10381 caggcggcga cgcaatatta tccgtcaccg caaatcactg tttatctgca aaaaacgctg
    10441 gacgatgacg ctgctgcggg cgtggtggca cagttgcagg ccgagcaggg cgtggagaaa
    10501 gtgaattatc tttctcgtga agacgcactg ggcgagttcc gtaactggtc tggttttggt
    10561 ggtgcactgg atatgctgga agaaaacccg cttccggcag tggcggtggt gatcccgaaa
    10621 ctcgattttc aggggacgga atcactcaat acgctgcgtg atcgtatcac gcagattaac
    10681 ggcattgacg aagtgcggat ggatgacagc tggtttgccc gtctggcggc gttgaccggg
    10741 ctggtcgggc gcgtttcggc gatgatcggc gtgttgatgg tggcggcagt gttcctcgtc
    10801 atcggtaaca gtgtacgcct gagtatcttt gctcgccgtg actccatcaa cgtgcagaaa
    10861 ctgattggtg cgacggatgg tttcatcctg cgtccgttcc tgtacggtgg cgcactgctg
    10921 ggattttctg gcgcattgtt gtcattaatt ttgtcagaaa ttctggtgct gcgattgtca
    10981 tcggcggttg cggaagtggc acaggttttc ggaacgaagt ttgatatcaa tggtttatcg
    11041 ttcgatgaat gcctgctgtt gctgctggta tgctcgatga ttggctgggt ggcagcgtgg
    11101 cttgccacgg tacaacattt acgccacttt acgcctgaat aataaaagcg tgttatactc
    11161 tttccctgca atgggttccg tagcagggaa agagaccccg ttgtctcttc ccggtatttc
    11221 atctctatgt cacattttgt gcgtaattta ttcacaagct tgcattgaac ttgtggataa
    11281 aatcacggtc tgataaaaca gtgaatgata acctcgttgc tcttaagctc tggcacagtt
    11341 gttgctacca ctgaagcgcc agaagatatc gattgagagg atttgaatga ctgacaaaat
    11401 gcaaagttta gctttagccc cagttggcaa cctggattcc tacatccggg cagctaacgc
    11461 gtggccgatg ttgtcggctg acgaggagcg ggcgctggct gaaaagctgc attaccatgg
    11521 cgatctggaa gcagctaaaa cgctgatcct gtctcacctg cggtttgttg ttcatattgc
    11581 tcgtaattat gcgggctatg gcctgccaca ggcggatttg attcaggaag gtaacatcgg
    11641 cctgatgaaa gcagtgcgcc gttttaaccc ggaagtgggt gtgcgcctgg tctccttcgc
    11701 cgttcactgg atcaaagcag agatccacga atacgttctg cgtaactggc gtatcgtcaa
    11761 agttgcgacc accaaagcgc agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg
    11821 tttgggctgg tttaaccagg atgaagtcga aatggtggcc cgtgaactgg gcgtaaccag
    11881 caaagacgta cgtgagatgg aatcacgtat ggcggcacag gacatgacct ttgacctgtc
    11941 ttccgacgac gattccgaca gccaaccgat ggcaccggtg ctctatctgc aggataaatc
    12001 atctaacttt gccgacggca tcgaagatga taactgggaa gagcaggcgg caaaccgtct
    12061 gaccgacgcg atgcagggtc tggacgaacg cagccaggac atcatccgtg cgcgctggct
    12121 ggacgaagac aacaagtcca cgttgcagga actggctgac cgttacggtg tttccgctga
    12181 acgtgtacgc cagctggaaa agaacgcgat gaaaaaactg cgcgctgcta tagaagcgta
    12241 atttccgcta ttaagcagag aaccctggat gcgagtccgg ggtttttgtt ttttgggcct
    12301 caacaataat caatttcccc tccgacaaaa cgccaatccc cacgcagatt gttaataaac
    12361 tgtcaaaata gctattccaa tatcataaaa atcgggtatg ttttagcaga gtatgctgct
    12421 aaagcacggg tagtcatgca taaaacgaaa taaagtgctg aacaacaaca tcacaacaca
    12481 cgtaataacc agaagaatgg ggattctcag gatgaacata aagggtaaag cgttactggc
    12541 aggatgtatc gcgctggcat tcagcaatat ggctctggca gaagatatta aagtcgcggt
    12601 cgtgggcgca atgtccggtc cggttgcgca gtacggtgac caggagttta ccggcgcaga
    12661 gcaggcggtt gcggatatca acgctaaagg cggcattaaa ggcaacaaac tgcaaatcgt
    12721 aaaatatgac gatgcctgtg acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa
    12781 cgacggcatt aaatatgtga ttggtcacct ctgttcttca tcaacgcagc ctgcgtctga
    12841 catctatgaa gacgaaggca ttttaatgat caccccagcg gcaaccgcgc cggagctgac
    12901 cgcccgtggc tatcagctga tcctgcgcac caccggcctg gactccgacc aggggccgac
    12961 ggcggcgaaa tatattcttg agaaagtgaa accgcagcgt attgctatcg ttcacgacaa
    13021 acagcaatac ggcgaaggtc tggcgcgtgc ggtgcaggac ggcctgaaga aaggcaatgc
    13081 taacgtggtg ttctttgatg gtatcaccgc cggggaaaaa gatttctcaa cgctggtagc
    13141 gcgtctgaag aaagagaata tcgacttcgt ttactacggc ggttatcacc cggaaatggg
    13201 gcaaatcctg cgtcaggcac gcgcggcagg gttgaaaact cagtttatgg ggccggaagg
    13261 cgtggctaac gtctcgctgt ctaacattgc gggcgaatca gcggaagggc tgctggtgac
    13321 caagccgaag aactacgatc aggttccggc gaacaaaccc attgttgatg cgatcaaagc
    13381 gaaaaaacag gacccaagcg gcgcattcgt ttggaccacc tacgccgcgc tgcaatcttt
    13441 gcaggcgggc ctgaatcagt ctgacgatcc ggctgagatc gccaaatacc tgaaagcgaa
    13501 ctcggttgat accgtgatgg gtccgctgac gtgggatgag aaaggtgatc tgaaaggctt
    13561 tgagttcggt gtgtttgact ggcacgccaa cggcacagcg accgatgcga agtaattgtt
    13621 aatcggcaac tttgggttgc cgccaaattg ctaatataga gtacgttgct tcatgccgga
    13681 tgcggcgtaa acgccttatc cggcctacaa gatccaaaga aatcagtaaa ttgcaacaca
    13741 cattgtaggc ctgataagcg tagcgcatca ggcaatttct cgttggtcat cacttttgaa
    13801 atcaaacttg acgattaacg cttctcccag ccgccctgct gtgccgtaaa tcccagtgcc
    13861 tgcataaacg ccgtcatcac accgcgatct tccacgcctg catccgccat ccaccagcat
    13921 gaaacgccag gattgttacg caaaacctct tccagcagat attgccccac accgcgacgt
    13981 cgagtgactt cccgcacgcg cagggaatcc agtgccccct cggtgccgct taaggttacc
    14041 cgcacggccg caagcaggcg ctcgttaaaa cgcgcggcgt agatgcggtg gttatcgtca
    14101 acctgtaaag aggaagggga atactccggc cagatctttt gcaggtcaat ccggtcctgg
    14161 tcgctaaatt tttctaatcg aatgatggtc agcttcatgg gtaacccgtg taaatcacaa
    14221 aagtggaacc agtgtagcga aataatttaa tcggaggctt tctctttttt atttcttttg
    14281 gcaggtgatt aattttttaa cagcaataat tacaaagtta aaacattaga gaatgaaaaa
    14341 tgtccagcat aatcccctga atgatagtga attattccgc c
//
LOCUS       DAZGBC010000125        14371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGBC010000125 DAZGBC010000000
VERSION     DAZGBC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.126"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(360..785)
                     /gene="arsC"
                     /locus_tag="ACHK1J_003632"
     CDS             complement(360..785)
                     /gene="arsC"
                     /locus_tag="ACHK1J_003632"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO7912852.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDQL
                     VKLIADMGLTVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1118..1291)
                     /locus_tag="ACHK1J_003633"
     CDS             complement(1118..1291)
                     /locus_tag="ACHK1J_003633"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912853.1"
                     /translation="MHPLTHPLPVTVHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHCQSENASELTG"
     gene            1319..1402
                     /gene="dinQ"
                     /locus_tag="ACHK1J_003634"
     CDS             1319..1402
                     /gene="dinQ"
                     /locus_tag="ACHK1J_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO7912854.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1456..2808)
                     /gene="gorA"
                     /locus_tag="ACHK1J_003635"
     CDS             complement(1456..2808)
                     /gene="gorA"
                     /locus_tag="ACHK1J_003635"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO7912855.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2880..3722)
                     /gene="rlmJ"
                     /locus_tag="ACHK1J_003636"
     CDS             complement(2880..3722)
                     /gene="rlmJ"
                     /locus_tag="ACHK1J_003636"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO7912856.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3925..5967
                     /gene="prlC"
                     /locus_tag="ACHK1J_003637"
     CDS             3925..5967
                     /gene="prlC"
                     /locus_tag="ACHK1J_003637"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO7912857.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5975..6727
                     /gene="rsmJ"
                     /locus_tag="ACHK1J_003638"
     CDS             5975..6727
                     /gene="rsmJ"
                     /locus_tag="ACHK1J_003638"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO7912858.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6776..8245)
                     /gene="dtpB"
                     /locus_tag="ACHK1J_003639"
     CDS             complement(6776..8245)
                     /gene="dtpB"
                     /locus_tag="ACHK1J_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO7912859.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGSKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMVAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8411..8464
                     /locus_tag="ACHK1J_003640"
     CDS             8411..8464
                     /locus_tag="ACHK1J_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912860.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            8442..8495
                     /locus_tag="ACHK1J_003641"
     CDS             8442..8495
                     /locus_tag="ACHK1J_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912861.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(8562..8996)
                     /gene="uspA"
                     /locus_tag="ACHK1J_003642"
     CDS             complement(8562..8996)
                     /gene="uspA"
                     /locus_tag="ACHK1J_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO7912862.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9387..9722
                     /gene="uspB"
                     /locus_tag="ACHK1J_003643"
     CDS             9387..9722
                     /gene="uspB"
                     /locus_tag="ACHK1J_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO7912863.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9793..11292)
                     /gene="pitA"
                     /locus_tag="ACHK1J_003644"
     CDS             complement(9793..11292)
                     /gene="pitA"
                     /locus_tag="ACHK1J_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO7912864.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11524..12726
                     /gene="yhiN"
                     /locus_tag="ACHK1J_003645"
     CDS             11524..12726
                     /gene="yhiN"
                     /locus_tag="ACHK1J_003645"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO7912865.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(13041..14093)
                     /gene="yhiM"
                     /locus_tag="ACHK1J_003646"
     CDS             complement(13041..14093)
                     /gene="yhiM"
                     /locus_tag="ACHK1J_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO7912866.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         3666 a         3607 c         3758 g         3340 t
ORIGIN      
        1 ttttgtgatg ttttagatgt gtaataataa aatgggttaa ggtatcttat aattattgaa
       61 taatttgttg tgtaaagcac tgataaatca taatccagta cgtctttaaa ctgctgcact
      121 gtatcgcgga taactaagca gctcactgat acaccatgct ggacgcgata attggaatct
      181 cacgcaggga gtaatgtgta aatagcaaaa tcgcctgatg cgctacgcct gtcaggccta
      241 caaataacat actattcatc gaattttcat aactttgtag gtcggataag acgcgccagc
      301 gtcgcatccg acataaataa agcgcacttt accaactatc ggcagtggat atcgccgctt
      361 tatttcaacc gcttacccgc ttcatcaacc actttctcgc catcttcctt ggagaacgcg
      421 cctttttgcg catctggcag aatttccagc accacttccg aggggcggca taggcgagtt
      481 cccagcggcg tcaccacaat cggacggtta atcaggatag gatgttgcag cataaaatca
      541 attaaccgat cgtcagtaaa tttatcttct gcaaggccca gttcctcata cggctcgacg
      601 tttttacgca gcagcgcgcg tacggtaagc cccatatcgg caatgagttt gaccagttga
      661 tcgcgcgttg gcggtgtttc cagataatag ataatggtcg gttctgtacc gctgttgcgg
      721 atcatctcca gcgtattacg cgacgtgccg caggccgggt tgtgataaat ggtaatgttg
      781 ctcatatcag tatctcatta caaagtgaaa gagagacgta gcgccagcgc agccagcgtt
      841 acaaaccaaa gcggataagt caaaaacata tatggtttaa cgaatgcgta tgtgcagagg
      901 aaggtaatag gtgtgaattt tgagttggct attcatttga aaggaggtcc gaatcaggac
      961 gtaaaaaaac cgactttgcg tcggtttttt actttccagc cctgagttgg tggctctggc
     1021 tggagtgaga tagccatcat ctaacgcatc aatgctaata ccatactgaa actattgcaa
     1081 ggacgtgctg gttttataac ctgcatgtac tgtatgatta tccagttagc tctgaggcat
     1141 tttcactctg gcaatgcgca taaacgcttt caaagtcctg gtcagaagta cgggtggtgc
     1201 cgttaactga tgctctggcc ggagtgagat agtcgtcatc taacaatgag acatgcaccg
     1261 tgacaggcag tggatgagta agcggatgca ttctcactcc atcgcatgga gaaaacgggt
     1321 gattgataaa gcaatcatcg ttcttggggc gttaattgcg ctgctggaac tgatccgctt
     1381 tctgcttcag cttctgaact gatagcggaa acgtaattaa gggctaagag cacactactc
     1441 ttagcccttt aacatttaac gcatggtcac gaactcttct gccgccgttg ggtgaatggc
     1501 gacggtattg tcgaagtctt ttttggttgc ccccatcttc agcgccaccg cgaagccctg
     1561 caacatttcg tccataccaa aaccaatgcc gtgaataccg acaatcttct cttctggccc
     1621 aacgcacacc agcttcatgc ggcacggctg gcggtgagtg gtgacggcgg tatacatcgc
     1681 ggtgaaagag gatttataca ctttcacctg atcgtcgccg tactgctcgc gcgcctgcgg
     1741 ttccgttaaa ccaacagtac caatcggcgg atggctgaag accacggtcg gaatgttgct
     1801 gtaatccaga tgctcatccg gcttgttatt aaacaggcgt tcagagaggc ggcgacccgc
     1861 tgcaactgcc accggtgtca gttctactgc acccgtgtta tcgcccaccg cgtaaatacc
     1921 ttcaatgttg gtgttttgat atttatcgac gacgatatag cctttttcgt tggttttaac
     1981 gccagcggct tcgaggttga tgttgtcatt ggcaggctcg cgaccaatcg cccaaatcag
     2041 gcaatccacc gtttcactgc gaccatcttc cagctccagc gtcaggctac catcggcatt
     2101 tttcactacc gctttcggga tggcgttggt gtgcaactgc gggccttcgg cgttcatcac
     2161 ttcaaccagc gtttcagaga tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac
     2221 aaacagatgc gttttcgcgc ccaggccgtt aatcacgccc gccagctcaa cggcgatgta
     2281 acccgcgcca acaaccgcca cgcgctctgg caaagcagga agggcgaaga agccatcaga
     2341 atcaataccg tattccacgc ccggaatatc cgggtggctc ggacgaccgc cggtggcgat
     2401 caggatatga tcggccgtga tggtttcgcc gtttacttcc agcgttttgg catcaacgaa
     2461 gcgggcaaag cctttgatta catcaacgtt atttttaccg agcacgtttt cataggaagt
     2521 atgaatacgg tcgatatagg cggtacggct ggcgatcaac gtttcccagt tgaatttatt
     2581 gatagtggta tcaaaaccat aatccgggcc gtacatatgg atcgcttcac ggatttgcgc
     2641 cgcgtgccac atcacttttt tcggcacgca gccaacattc acgcaggtgc cgcccagctc
     2701 tttggcttca atcagcgcgc atttctggcc gtacatagcc gcgcggttga tggaggcgat
     2761 accgccgctg ccgccgccga tggcgatgta atcatagtgt ttagtcatga cagagtgtcc
     2821 ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag gtgccagcaa tggctgcaat
     2881 tactccggca cgatccagct tacggtggcg tgcccggtgc cagccggaac cagtttgctg
     2941 tgcagccacg gcagcacgtt attcatctgc tgttccagct tccacggcgg gttaatcaca
     3001 atcatgccgg aagcggtcat gccacggcga tcgctgtctg gcagtaccgc cagttcaatt
     3061 tgcagaattt tgcgaatacc ggtcgcttcc agatcgtgga tcatgcgctt aatttgctga
     3121 cgcagcacca ccggatacca cagtgcgtaa gtaccagtgg cgaaacgttt gtaaccttct
     3181 gctatcccgc tgaccaccgc ttgatagtcg gttttcattt catacggcgg gtcgataagg
     3241 attaaaccac ggcgggaaac cggcggcagt ttggccttaa gctgctggaa accgtcggat
     3301 ttttcgacgc gcgcacggct gtctttctga aattcagaac gcagcaacgg gtaatcgctc
     3361 gggtgcagtt cggtcagttg caggctgtcc tgttcacgca gtaactgacg ggcaatcaac
     3421 ggtgaaccgg ggtagtaacg caactgaccg ctacggttga agtgttttac cacattgatg
     3481 tacgcctcca gctccgcagg caaatcgtcc tgctgccaga tacgggcgat accttcgaga
     3541 tattcgccgg tacgctcggc atgttcgcta cctaactgat aacgcccggc gcctgcgtgg
     3601 gtgtcgagat agagaaacgg tttatctttc tctttcagcg actcgatgat caggctctga
     3661 acggtatgtt taagtacgtc ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc
     3721 atgggtaaag gtgttccggt aagtaaaaat cggcccgtat tcggggcgca caaaagccta
     3781 tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc gtaacgtttc tcattgaaat
     3841 tcactacact taaccccatg ctacacacat tatgtaaagc gcctgttgag cgcttcctta
     3901 acctctttaa ccaggactgc gctaatgacg aatccgttac tgactccctt tgaattgcct
     3961 ccgttttcta aaattctccc ggaacatgtc gttccagccg tgactaaggc attgaatgac
     4021 tgccgcgaaa atgtggagcg cgtagtagcg caaggagcac cgtacacctg ggaaaatctc
     4081 tgccagccgt tggcggaagt ggacgatgtg ttggggcgta ttttctcccc ggtcagccac
     4141 ctgaactcgg tgaaaaatag cccggaattg cgcgaagcgt acgaacaaac tctgccgctg
     4201 ctgtcggaat acagcacctg ggtagggcaa catgaagggc tgtataaagc gtaccgcgac
     4261 ctgcgcgatg gcgatcatta cgccacgctg aacacggctc agaaaaaagc ggttgataac
     4321 gcactgcgcg attttgaact ctcaggcatc ggtctgccga aagagaaaca acagcgttac
     4381 ggcgaaattg ccactcgtct ttctgaactg ggcaaccagt acagcaacaa cgtccttgat
     4441 gcgacgatgg gctggaccaa actcgttacc gacgaagcgg aactggcggg gatgccagaa
     4501 agcgcgctgg ctgcggcaaa agcccaggcc gaagcgaaag agctggaagg ctacctgttg
     4561 acgctggata tcccaagcta cttgccggta atgacctact gcgacaacca ggccttgcgt
     4621 gaagagatgt atcgtgctta cagcacccgc gcttcagatc aaggtccaaa cgccggtaaa
     4681 tgggataaca gcaaggtgat ggaagagatc ctcgctctgc gtcacgaact ggcgcaactg
     4741 ctgggttttg aaaactacgc ctttaaatcc cttgccacta aaatggcaga aaatccgcag
     4801 caggtgcttg atttcttaac cgatctggca aaacgcgcgc gtccgcaagg tgaaaaagag
     4861 ctggcgcaac tgcgtgcctt cgctaaagcc gaatttggcg tggatgagtt gcagccgtgg
     4921 gatatcgcgt actacagcga aaaacaaaaa cagcacctct acagcatcag cgacgagcag
     4981 ctgcgtccgt acttcccgga aaacaaagcg gttaacggcc tgtttgaagt ggtgaaacgt
     5041 atttacggca tcaccgctaa agagcgtaaa gatgttgatg tctggcatcc ggatgtacgt
     5101 ttcttcgaac tgtatgacga gaacaacgaa ctgcgcggca gcttctacct cgacctgtat
     5161 gcccgtgaaa acaagcgcgg cggggcgtgg atggatgact gcgtaggcca gatgcgtaaa
     5221 gccgacggtt cgctgcaaaa accggtcgcg tatctgacct gtaacttcaa ccgcccggtg
     5281 aatggtaaac cggcgctgtt tacccatgac gaagtgatca ccctgttcca cgagttcggt
     5341 catggtctgc accatatgct gacccgcatc gaaaccgccg gtgtatccgg gatcagtggg
     5401 gtgccgtggg atgcggtcga actgccgagt cagtttatgg aaaactggtg ctgggagccg
     5461 gaggcgctgg cgtttatctc cggtcactat gaaaccggcg aaccgttgcc gaaggagttg
     5521 ctggataaaa tgctggcggc gaagaactac caggcggcgc tgtttattct gcgccagctg
     5581 gagttcggcc tgttcgattt ccgcctccat gccgagttcc gcccggatca gggagcgaaa
     5641 atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg tagtaccgtc tccatcctgg
     5701 ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg gttatgccgc aggttattac
     5761 agttacctgt gggccgacgt gctggcggca gatgccttct cgcggtttga ggaagagggc
     5821 attttcaacc gtgaaaccgg gcagtcgttc ctcgacaaca ttctgagccg tggcggttca
     5881 gaagagccga tggatctgtt caaacgcttc cgtggtcgtg aaccgcaact ggatgcgatg
     5941 ctggagcatt acggcattaa gggctgatca ttcagtgaaa atctgcttaa ttgatgaaac
     6001 aggcaccgga gacggtgcct tatctgttct ggcggcccgc tgggggctgg agcacgatga
     6061 agacaacctg atggcgctgg tgttaacgcc ggaacatctg gaattgcgca agcgtgatga
     6121 gccaaaactt ggcggcatct ttgttgattt tgttggcgga gcgatggcgc accgacgcaa
     6181 attcggcggt ggtcgcggtg aggcggtggc gaaagcggtg ggcattaaag gcgattattt
     6241 gccggatgtg gtggacgcta ccgcagggct ggggcgcgat gcctttgtgc tggcttcggt
     6301 cggctgccgc gtgcggatgc tggagcgtaa tccagtcgtt gccgcgctac tcgacgacgg
     6361 cctggcgcgt ggatatgcgg atgcggaaat cggcggctgg ttgcaggagc gtttgcagtt
     6421 aattcacgcc tccagcctga cggcgctgac cgatattact ccgcgcccgc aggtggtcta
     6481 tctcgacccg atgttcccgc ataagcagaa aagcgcgctg gtgaaaaaag agatgcgtgt
     6541 ctttcagtcg ctggtggggc cggatctcga cgcggatggg ttgctggagc ctgcgcggct
     6601 gctggcgact aaacgcgtgg tggtaaagcg tccggactac gcgccaccgc tggcgaatgt
     6661 cgccacgcca aacgcggtag tcactaaagg gcatcgcttt gatatttatg ccggaacgcc
     6721 ggtgtaaaag taaagcaggg tagcgataac gctaccctgc tttctgcaag aataattaat
     6781 ggctttccgg cgtcgcaatc atgcgtttca gccacggcac catcaacagc atcactactg
     6841 caacgcccag cgtgaccaga ccaatcttac caaacacgtt ggtatagacg ggcaacgtct
     6901 caagcggatc ggtaatgttg tccggcaccg cggtaaatgt tgccacatag ccgcccagca
     6961 agaacgcggc agcctgcgtc aggaaccaca tcccgagaat aaagcccatc aaatgctgcg
     7021 gcaccagagc ggcaaccatc gccagaccca gggcgctaat aaacaattca cccaggctct
     7081 ggaacaagta aaccagcacg ataaaccacg gcgatgtcag tccttgtgca tcagcaaacc
     7141 acattcccgc agcggcggcg gtaagaaagc ccagcgagca cataaacatg ccgagagtaa
     7201 atttcatcgg catagagaga tctttgcctt tgctgcccag atgcgtgtaa atgcctgcca
     7261 gtattgggct ggcgagtacc acccagaacg ggttaagcgc ctggaagctg accgggttga
     7321 tggaaaagcc gagaatttca tgatgcacgt tgttgatggc aaagaagttc agcgacgttg
     7381 gcatctgggc gtagagaatg taaaacacca ccgcttcgag catcaggaca aaggcgacaa
     7441 acattttatt gcgcccggtt ttatccagct tgaatgcctg acgaaagaag atgatggtga
     7501 cgacgatgga gagaacaatc agcaccagat tggcgacttc tacgttgtgc atcagccatg
     7561 cacagacgaa gatcatcacc acgctgccaa gtaacacgta caacagtttg ctgaagctca
     7621 tcggccggaa gtcgggttca gaaccaatgt ctttcaccat tccacgacag gcgatgtaaa
     7681 ccagtaatgc gataattaac cccgccccgc acaggttgta ggtgacagaa taaccgaatc
     7741 tatcagcgat cacaggggcc agcgataacg ctatcaacga gccgatgttg atcgacatat
     7801 agaacagggt gaatgcgcca tcaaggcgcg gatctttcgg cggatagcac ttcgaaagca
     7861 agctggctgg gttagcttta aacaggccgt taccgacagc gatagtcccc agggcgatga
     7921 aaatcaggtc aggcttaagt agcgacaggc cggtcatgaa gtagccaatc gccagcacaa
     7981 gtgctccgag aacaatggtg cgtttggtcc ccagcaggtg gtcgccgaca tagccgccaa
     8041 tggaaatgag gccatagacc agcgcagcaa aagcaccaaa agtgacaaaa gcctgctctt
     8101 gcgagaatcc aagctgttta acgaagaaaa ccgccagtac gccctgcacg ccgtagtagc
     8161 cgaatcgctc ccataactcg acaaaaaaga tcatgaaaaa tgggcgaggt tgctgcagca
     8221 tccccatggg tgttgttgta ttcatattta ttaaccttcc aataccatcc tgaaaagtat
     8281 gaacgcgtaa gggataacgc gcgaaaaagt ttacatctgt cagagattct taatattgat
     8341 gcggtgaata gattaataca ctattacaac agaaaataac cagctgatca cactaatgat
     8401 ggaaaatatg atggtgttaa atattgtatt ggctggggat tttgattgcg aacgcagacc
     8461 gtaggccaga taaggcgttt acgctgatca ggtaacaagg cgcgccaaaa agcaaaacgc
     8521 ccgcatatgc gggcgttcag gaacacgttg tggagggaag attattcttc ttcgtcgcgc
     8581 agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc
     8641 agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc gtatttcttg
     8701 attgcatcga ccagaacctg gcccaggtcg ccgctgccgc tcagggtttc agtgattggg
     8761 tatcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc
     8821 tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta gtttacatct
     8881 acgtggatca gagaaacttt cgcattgtat gggcgagcca tagagactgc tttctctacc
     8941 agaactttgc tttccgggga gagatcgacc gcgatgagaa tgtgtttata agccatagtg
     9001 ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg ccgttacgtc
     9061 acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag
     9121 accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt acccgttaga
     9181 taaacaaatt ttcgacatga atatattgat agtggttaac cttctggaaa aaaaacaacc
     9241 tgatctccta cactatctat agagccgctc ggatgttggt caccacaaaa gcggtttctc
     9301 caggacctgg tgttaactga ttggcagcgt atcgggagcg gtagccccgg ggtggaactc
     9361 cgcgggcagg tcgccgggga ggaggtatga taagcaccgt cgcattattt tgggctttat
     9421 gtgtcgtttg cattgttaac atggcgcgct atttctcatc actacgcgcg ttgttagtgg
     9481 tactgcgtaa ctgcgatcca ttgctctatc aatatgttga tggagggggc ttttttacct
     9541 cacatggcca acccaacaaa caggtgcgtc tcgtttggta tatctatgcc caacgttatc
     9601 gcgatcatca cgatgatgag tttattcgcc gctgtgagcg ggtgcgtcgg cagtttattc
     9661 tgaccagcgc attgtgtggt ctggtggtgg tcagcctgat tgcattgatg atttggcatt
     9721 aagcacttca gcaataataa cgcataaaaa aagcgggcca gtaaactgac ccgctcgttt
     9781 tggtgtgtac gattacagga actgcaagga gagccagtac agcccgccgg aaagcagtac
     9841 cgcagccgga agggtaaaca cccaggccat cagaatactg gttacggttt tacgctgtaa
     9901 gccgccgcca tctaccacca tcgtccccgc gacagaagag gagagtacgt gagtggtgga
     9961 aaccggcatc ccggtataac tcgccaggcc gatagacact gccgccgtca tctgggcaga
    10021 catcccctga gcgtaggtca tgcctttctt accgattttc tcaccgatag tcgttgccac
    10081 acgacgccag ccaatcatcg taccgatacc taacgccagc gcgaccgcca tgatgatcca
    10141 caccggcgca tactcgatgg tgctaagcat gtcggacttc agtttcttca acaggcgctg
    10201 atcgtcagca ctcacaccag gcatcttcac cactttgtcg atagtgtcag aaacgcacag
    10261 cataatgcgg cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc tttccacatc
    10321 ggtagtcaac atgcctttca ggcggttgag cgcgttaatg gtattcgacg gatggcaatg
    10381 aaactccgca ggttgcgttg cgccagcttc cggagccggt actaactgat cagcaccggt
    10441 agcttgtttc agcagcgcag gatgctgctc aaagtaagct tcgacgttgt tgatggcatc
    10501 acgggtacgg gtgatttcgt agccagtggc attcatgttc accacgaagc ctgctggtgc
    10561 gacaccaatc aataccaaca taaccagacc aatgcctttc tgaccatcgt ttgcgccgtg
    10621 cgaaaacgcc acgccgatag cggaaaggat cagcgcgata cgcgtccaga acggcggctt
    10681 tttcttgccg tctttctttt cacgctccgc tggggtcagg tggatacggg cgcgtttctt
    10741 ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca ccaggccgac
    10801 aataggggaa acgatcagag aaccgaaaat acttaatact ttcgggatat tgagtgcatc
    10861 caccactgac gtcccggtca tcaacgcatt ggttaaacca atcccgatga tcgcgccaat
    10921 cagcgtatga gagctggatg caggtaaacc aaagtaccag gtacccaggt tccagataat
    10981 cgccgccagc aacatagaga acaccatggc aaggccatga gacgatccca tattaagcag
    11041 cagatccgtc ggcagcatat gcacaatagc ataggcaaca ctcagaccac ccagcaaaac
    11101 acccaaaaag ttgaacaccg ctgccataac cacggcgagc tgagaacgca tcgcgcgggt
    11161 atagataacg gttgccacgg cgttggctgt gtcatggaaa ccattgatgg cttcgtagaa
    11221 cagcacaaaa gccagtgcaa gcaataataa cagcccggta tgcaaatcca ggccagcaaa
    11281 caaatgtagc ataggacgtt acgccatttt gaggacatga acgcggcgca ttatcagtga
    11341 ctttcgcggc gcgggcaaag tgaaatatag actttttttg atttgcctcc tgtatggatt
    11401 tcactcaaaa aataattatc ttatataatt caggcaaata cttcctttta gtaatattga
    11461 tgctggtgcg accactgagg aatctttaca attcacgccc gttttttcta agaggagcgc
    11521 aacgtggaaa ggtttgatgc cattattata ggcgctggtg cggcgggtat gttctgttct
    11581 gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa aaaaccaggg
    11641 cgcaaaatcc ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta tgtcgaaccg
    11701 ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg ttttacccag
    11761 tgggatttca ttgatctggt caataaacac ggcatcgcct ggcacgagaa aacgttaggg
    11821 caactcttct gcgatgactc cgctcagcag attgtcgaca tgctggtgga tgagtgcgag
    11881 aagggcaatg tgaccttcag attgcgtagc gaagtgctga gcgtggagaa ggatgataca
    11941 ggcttcacgc ttgaactgaa cggcatgact gtcggttgcg aaaagctggt catcgcgacc
    12001 ggcgggctgt caatgccggg cctgggcgcg tcgccgtttg gttataagat tgccgaacaa
    12061 tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc cattcactct gcataaaccg
    12121 ttgctcgaag agttacaggt gctggcaggc gtggcggtgc cttccgtgat taccgctgaa
    12181 aacggcaccg ttttccgaga gaacttactc ttcacccacc gcggcttatc tggaccggcg
    12241 gtgttgcaga tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa tctgctaccg
    12301 gatgtggacc tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa tcagagcctg
    12361 aaaaacacac tggcggttca tctaccgaag cggttggttg aacgcttaca gcaactcggg
    12421 caaatcccgg atgtctcgct aaaacagctc aacgtgcgtg accaacaggc actgattagc
    12481 acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac tgccgaagtg
    12541 acgctcggcg gcgtggacac taacgaactc tcttcacgga cgatggaagc gcgcaaagtg
    12601 cctgggctgt acttcatcgg cgaagtgatg gacgtcaccg gctggctggg gggctataac
    12661 ttccagtggg cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc agcgaagtcg
    12721 tcctgagctt tttcctaaag atgaaaatta ttagagtcgc attaaaaatg agctaatttt
    12781 gatagtggct atcttgtgat tattttctaa tgagcccgtg aactgaaccc cccatgctta
    12841 atataaggtg gagggaaagg tgattgaaaa ctcacccagt gggctgatct ttataaaata
    12901 tgaacaggtt gaaaaaaaac agtaactttg ctgtttttta tacttaacca ctatgcatta
    12961 atgctgcgtt atttcatgat acctaagaaa aaccagcgtt acgcaaatgg tcaacgctgg
    13021 ttttatccgg tacgttgcaa ttatttttta gcagaacccg cttctaatat tgaaacgatt
    13081 gagaacaacg taaagcatac cgctcccaaa ccgaccagaa cccgcgaagg aataaaatat
    13141 cccatatcgg tctgcgccat ttccgcaaga aacgatgcca ggaaaaggca aaacaggcag
    13201 gtgaagacgg gaatcatcgg tattctgttg gctaacgaac aggtacgtct ccagaccagt
    13261 gccagtagcc agacttttga gaatatgctg taacagatca ttccgaggca aatgagaata
    13321 atgccgggag ccagtcgggc gcttgcatcg gagctgacta agacgtatat gccctgcagc
    13381 accgtgattg agccgagaaa aataacccaa taacaccaca gccagtgctc tttcgttgaa
    13441 aacgtattac gtgtttgatg gacgattgtg gcaacaaggc caattagaca ggcacagatt
    13501 gcggttaacc cgagcaatac gtgaccggcg acataatgcg gagttatgtc ggcactacgt
    13561 aacagcgtaa tagaccaaat gaaaccgagc agggtgagta acacgggaac ggcaatgagg
    13621 caattaccga ttaatgaaga ataagcctgt accggtgttc cgtcgctctt gctccccgct
    13681 gcgtttttag gaatgagcag aaagtgaccg gatgacgctg ccactgtcga tacacaggcg
    13741 gcaatcatac caacgccgaa aataacatgg ccggcgacaa actcgtctgc catcacatca
    13801 ttacctgcta acagtgccca accccatatc atggtgataa ttgaccccgc atagccaata
    13861 atggggaaca atgtattgta aaaagtatta acgccgcgcg tgagctgcga aatgataata
    13921 aatgcggtag tgaataatgc caggcatatg gctgccagag aaatgagcac atgacccgcg
    13981 acgaaatact cgctttgccc tgaactttcc agcacaaagc cacctaacgc gatacaaatc
    14041 aagcccataa ttaaagggat taatttgaaa agccacccga tatatatgtt cactttaaac
    14101 tcctcaggta tccctgctaa tattgaaatg tgcattaatg ataatttcag cctgttaata
    14161 caatgacgag atatcattaa agacaaatgc atagcataac tatagcgggg tttactttgc
    14221 agagcgctac ttttcagcgt tcaaaaggcc atgataaata aatatttatc ctaagattaa
    14281 ttcttattct gttgtttttt acaattgtct tccagggagg gattcttttt cattatttgg
    14341 taaaagaccg gatggtctcg gtgcttaaaa t
//
LOCUS       DAZGBC010000126        14309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGBC010000126 DAZGBC010000000
VERSION     DAZGBC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.127"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..439
                     /locus_tag="ACHK1J_003647"
     CDS             83..439
                     /locus_tag="ACHK1J_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7912867.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(490..1386)
                     /gene="yhaJ"
                     /locus_tag="ACHK1J_003648"
     CDS             complement(490..1386)
                     /gene="yhaJ"
                     /locus_tag="ACHK1J_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO7912868.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1491..2192
                     /locus_tag="ACHK1J_003649"
     CDS             1491..2192
                     /locus_tag="ACHK1J_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO7912869.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLRKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2215..2379
                     /gene="yhaL"
                     /locus_tag="ACHK1J_003650"
     CDS             2215..2379
                     /gene="yhaL"
                     /locus_tag="ACHK1J_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO7912870.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2513..3823)
                     /gene="cyuA"
                     /locus_tag="ACHK1J_003651"
     CDS             complement(2513..3823)
                     /gene="cyuA"
                     /locus_tag="ACHK1J_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO7912871.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVVEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3851..5182)
                     /locus_tag="ACHK1J_003652"
     CDS             complement(3851..5182)
                     /locus_tag="ACHK1J_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7912872.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5457..6821)
                     /gene="tdcG"
                     /locus_tag="ACHK1J_003653"
     CDS             complement(5457..6821)
                     /gene="tdcG"
                     /locus_tag="ACHK1J_003653"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7912873.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6893..7282)
                     /locus_tag="ACHK1J_003654"
     CDS             complement(6893..7282)
                     /locus_tag="ACHK1J_003654"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO7912874.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7296..9590)
                     /gene="tdcE"
                     /locus_tag="ACHK1J_003655"
     CDS             complement(7296..9590)
                     /gene="tdcE"
                     /locus_tag="ACHK1J_003655"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO7912875.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9624..10832)
                     /gene="tdcD"
                     /locus_tag="ACHK1J_003656"
     CDS             complement(9624..10832)
                     /gene="tdcD"
                     /locus_tag="ACHK1J_003656"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO7912876.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10858..12189)
                     /gene="tdcC"
                     /locus_tag="ACHK1J_003657"
     CDS             complement(10858..12189)
                     /gene="tdcC"
                     /locus_tag="ACHK1J_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO7912877.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12211..13200)
                     /gene="tdcB"
                     /locus_tag="ACHK1J_003658"
     CDS             complement(12211..13200)
                     /gene="tdcB"
                     /locus_tag="ACHK1J_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO7912878.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVARPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13299..14237)
                     /gene="tdcA"
                     /locus_tag="ACHK1J_003659"
     CDS             complement(13299..14237)
                     /gene="tdcA"
                     /locus_tag="ACHK1J_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO7912879.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3692 a         3841 c         3351 g         3425 t
ORIGIN      
        1 catccgaaaa agaaagatac gaatagcatt tacacaatac ccggaataga ctttcgtcac
       61 ttttaattaa agggatgttt ttatgcagtg gtatttatcc gtattaaaaa attatgttgg
      121 tttctctggt cgcgcacgtc gtaaagagta ctggatgttt actctgatta acgccatcgt
      181 cggcgctatt atcaatgtca ttcaattgat tttaggtctg gagcttccat atctgtctat
      241 gctttatttg ctggcaacct tccttcccgt ccttgcgctt gctatccgtc gcttacacga
      301 taccgaccgt tcaggtgcct gggccttgct cttttttgtt ccgtttattg gctggctcgt
      361 gcttctggtt tttttctgca cagaaggtac ttctggcagc aatcgttacg gaaacgatcc
      421 gaagtttggt tcaaattaat cttagaattg gggcgatatt tcgccccttt ttattaacaa
      481 taataaatat tattttccgt taaaaagttt gggaatttcc cgcagacacc aggatttcgc
      541 ttcccccata ctgtcacgac gccaggccat aataatatcg atctcgctgg tcgattccgg
      601 gctgacgaca cgcaaccgcc cttccgcaat atctttttcg accatcggat acggcatcgt
      661 cgccacgcca agccccgcca gtaatgcctg acgtttatct tcaatcgtgc tcaccgttaa
      721 gcgcggctgt ttgtccagca gctgtacggt caacaccggg cgctcacgag cggtatccgc
      781 caccgcaatt ccacgatatt tcacacgcgt cacttcagat aacggttccg gctcctgatg
      841 aatcgggtga tcaggcgcgg caacgtagac gttcattaac gtatagagct tgcgcgagtt
      901 gatctccgac gaggaacgaa aatgcatatc cggcgcgata acaatatccg cccgcccctg
      961 ctccagccgt tcccacgccc ccgccagcac ttctgtgatg attgccagtt gggtattggc
     1021 ttttgccgcc agtttgtcga ttaacgggaa aaaggcaggt gtcggtacca gcgcttcggt
     1081 cacaatggtg agatgcgttt cccaaccgcg cgcgagagct tccgcatcag tagtcagttt
     1141 atctgcggct tccagcaaaa cgcgccctcg ctccaacaac atcctcccga cattggtgaa
     1201 tttggtgcga tggcccgagc ggtcaaacag caccacatcc agctcttctt ccagtttttg
     1261 catggtgtag ctaagtgcgg aaggcacgcg tcccagctca tccgctgccg ccgcaaaact
     1321 gccccggcga tcgatcgcat ccataacccg tagtgcttcc agcgttaatg ccctttcttt
     1381 ggccatttcg ttctcattca ggaaatttga acataccggg cagaatatct ggctaacaat
     1441 gtagcgtcca gccccttacc ataaaaggaa gtaaagagag gtcaagaatt atgattacta
     1501 cccgaactgc caggcagtgt ggacaagcag actacggatg gttgcaggcc cggtatactt
     1561 tttcctttgg acactacttc gacccgaaat tgttaggcta tgcctccctg cgtgtgctta
     1621 accaggaagt gctggcccca ggtgccgcct ttcagccgcg aacctatccc aaagtcgata
     1681 ttttaaatgt gattctggat ggagaagcag agtatcgcga cagcgaaggc aatcatgttc
     1741 aggccagcgc cggtgaggcg ttgctgctct ctacccagcc gggtgtcagc tacagcgaac
     1801 acaatctccg caaagacaaa ccgttaacac gaatgcagct ttggctggac gcctgcccgc
     1861 agcgagagaa tccgctgatt caaaaactgg cgcttaatat gggcaagcag caattaatcg
     1921 cctcgccaga gggtgcgatg ggaagcctgc aattacgcca gcaagtgtgg ctgcaccata
     1981 tcgtgctcga caaaggcgaa agtgcgaatt tccagttgca tgggccacgc gcgtatttgc
     2041 aatcgattca cgggaaattt catgcgctta cgcatcatga agagaaagcg gcgctgacct
     2101 gcggtgatgg ggcgtttatt cgtgacgagg ctaacattac gctggttgcc gattccccac
     2161 tgcgcgcttt gctgatagat ttgcctgtct agttgttttt acagggagat gatgatgagt
     2221 aaaaaatcgg ccaaaaagcg ccagccagtg aagcccgtgg tggcgaaaga acctgctcgc
     2281 accgccaaaa attttggcta tgaagagatg ttgagcgagc tggaagccat cgtcgcggat
     2341 gctgaaacgc gtttagccga ggatgaagct accgcgtaaa gacaggttgc cggatgcggc
     2401 gaaaacgcct tatccggcct acaaaatcca acaacattaa cgaattaaac aacccgtagg
     2461 ccggataaga tgcgccagca tcgcatccgg cacgataccc aaaacctggc gtttatctgg
     2521 ccttgctcgc cataatctcg ataatctgcc gatccgtttg ctgcatcgaa tggcttgcta
     2581 atgcacacag gttggcaatc gactgctcaa catcatgcgc cacaatccct tcattgccgg
     2641 tcacggcggt atcatccagc gccattaaca ccgctttcca cgcagccgaa gcactggtcg
     2701 aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat catgccgctg acatcgccga
     2761 tcatactgct gatcgccatc gagatggttt cataacgccc atccaccagc catgccatcc
     2821 cggcggcggc ccccattgct gcggtcgttg cggcacacag cgcagacaaa cgcggtaact
     2881 ggttatggat gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc agccgttcat
     2941 catccgctcc gaagtgttct gctaccacca ccacaggcat tgttgcggtg atcccctggt
     3001 tacccgagcc ggagttactc atagccggaa gcgtagcgcc gcccatacgc gcatcggatg
     3061 ccgcgctggt acgaatcaca atggatgaag agagatcttt cgccagcaaa ccgcgcgcgc
     3121 actgtttttc cagcgtcgcg ccaatatgca gcccccactt accgctcaaa ccttcctgcg
     3181 ataacgcgca atttagcttc gcggaatcga gaataaagcg gatcgccgca aacgggactt
     3241 cattgacgaa cttcaggatc tcagccagcg tcgttctgga aagcaccgtc agcggagact
     3301 cttgctcgcc ctctaccaca cacgcctgct gggtaaacac cacaccatcg tgcgtctcaa
     3361 tatgcacaat gttggtatgc ccgccgacga tggtgacaca cgcccacttc tcaccgttcc
     3421 agactttggc gcgtgagaag aggatttcat tgcaaggttc ctggatctta acggagactt
     3481 tccccgccgc cagcagtgct ttggcatcgg caattgcctg cgcagttgcg tctttcagca
     3541 cttccagccc ggcgttggca tttccaccta acgcccccag cgccgccgca atcggcagcc
     3601 ccaccattcc cgtgccggga acggtgacgc ccagaccgtt cttcatcaga tttggcgaaa
     3661 cccaggcttc tacacgttca accggacctt ccagttctgc cgcagcaacc gccgccgcca
     3721 gcgccagtga aatcggttca gtacatccca gcgccggttt tacttcctcc tgaacggcga
     3781 ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa catattaaaa accttaaaat
     3841 ttcaggtaaa tcaagaaaat gccaggaacg gagaaacaca aagcaacaaa ccagtgacga
     3901 taatcaggta cagagacatc cctttgtatt tgtgcaatgc cggtactttg taaaccagcc
     3961 acgccgggat caggcaccct accatgccga aaatcgggct acagatagag gtgaagctca
     4021 acaccggtgc gttcagtacg atggcgctcc aggccagcaa aatggcgaaa atcatgatgc
     4081 cgcgctgaac aagattttcg ttaatcttct cggcaggcat cttgcgacgc aggatgttca
     4141 ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat gcagtcatga
     4201 cagcaaagat attgagaatg acgctgacca ctttcaccca cgctgcgccg tcaccgctaa
     4261 taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc gtaggctttt accgcttcgt
     4321 catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca aacaaaatgc
     4381 caaacgcgat attcattgcc cgcaatgctt tatgccgcgc cacttcaatt gatttttccc
     4441 gcgagcgata agagatcacc atcgggctta acgtctggat aaacagaatc gacgtcaggg
     4501 taaacggcag cgtaataatg gcgtttttca ccagcagccc cagcggcggt agcgaaccga
     4561 cgttatacag atgccacatt cccaccatcg acacgcccag cgccgcgacc accagcagct
     4621 ttgtcagcac catgccagtc gaaattttga ataacaattt ctcgccgcgt gaagagatcg
     4681 ccaccagaat gcaaatcagt accagaccat aaaagggact gtctgacagc aacccttccg
     4741 tcacgccgaa ggtatgcagg taggaagcac tatcgttggt gatggcggtg gaatagacga
     4801 acatccaaat caccagcatt acgaaataga gtgcacctaa caggatgccc cagtttttac
     4861 ctaaataacc gctaatgacg ctcgggtaat ctttacattc tggtgattct gccagcgtat
     4921 taataaacaa ccgctgaaac agatacattg ccgggtaacc aatcaccgat gagagcaaaa
     4981 atacccacaa tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc gcgccaatcg
     5041 ccatcccgat actcataatc acccagccgg tgtcagtact gtcgaatttg atcgcttctc
     5101 gccactcgct ctcactcatt ccagcacgac ccgccggggt cgaagcgtct gcaatgacgc
     5161 ctttattcga tgcaatttcc ataatttctc gctcaatatt ttgtagggct tattattttt
     5221 ttccgagccg catcaaggcg atatgcggtc tacgtttttg caggcgagtt attagaataa
     5281 agaaatgata cgcgcgaggt cagagaaaat cttcacaatc aagactttga aataacggag
     5341 atggataaga attttctact aaattaatcg caagagaata ttcattctct atttgcggca
     5401 gatcacaaaa aaaaggtgca catttgtgca cccaaggatg aaagctgaca gcaatgtcag
     5461 ccgcagacca ctttaatggc cagtcctccg cgtgatgttt cgcggtattt atcgttcata
     5521 tctttgccgg tttcatacat cgtctcgatc actttatcga gtgaaacacg cggtgccgag
     5581 gtgcggcgca tcgccatccg cgcggcgttt actgctttca cggcattaat ggcattacgt
     5641 tcaatgcacg ggatttgtac ctgtccggca accggatcgc aggtcagtcc aaggttatgc
     5701 tccatcgcga tttccgccgc attgcatacc tgcgccggac tgccgcccag tagttcagtt
     5761 aaccctgccg ccgccattga acaggccacg ccaatctccc cctgacagcc gacctccgcg
     5821 ccagagatgg aggcgttcat tttatacagc gcgccaatag ccccggctgc aagaaaatag
     5881 cgggcaattg accgctcgtt taccggacga cggaacttat cgtaataagc cagcactgcc
     5941 ggaataatgc cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc agctgcgttt
     6001 tcttcactaa ccgccagcgc gtacatgttg atccagtcga tgacattcat cggatcgtta
     6061 gagatgttat cgctggaaac cagttgacga cgcagcgcta cggcacggcg cggcacattg
     6121 agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc atgcatcact
     6181 tgccagatac gggcaaaacc ggcgtcaatt tccgctttac tgcgcagcgc tagctcgttg
     6241 tgcatcatca ggccagaaat agacaggccg ttgtaatcac acattttcag cagttcacct
     6301 gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc gaagtgttct
     6361 tcttcgacaa taaacccgcc gccgacagag taataggttt tacttaatag ctcttcctgt
     6421 gccttccagg cagtgatccg cattccgttc tcatggcgag gcaacatttc gggatggaag
     6481 ataatgttct ttgctacagg aaaatcaaca atatgcgcac cagatgccac tggcagccgc
     6541 ccgctgcgcg ttactaactc tataaatgca gggatctcat caatgacaac atcctgcgga
     6601 ctgtttcctg ccagtcccat gatgatggcg acatccgtgg catggccttt gcccgtcagt
     6661 gacaacgacc cgtacagatc gaccacaata tggctcgtcg cggttaataa gccgctactt
     6721 tccagccgat caataaaact ttttccggca ttcattggcc ccacggtatg cgaactggag
     6781 ggaccaatcc caattttgaa aatatcgaat gcactaatca tatccacacc ctcggattgc
     6841 cgttcagtga agtggagcgg aacgacctta cgaccgtccc gctcacgagg ctttacgcac
     6901 tacgtactgc gatagcttca atttccagct tcacatcttt cggcaaacgc gcgacctgta
     6961 cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta tagacttcgt
     7021 tgatggtggc aaaatcattc agatcggtga tgaacacggt catcttgatg atatcgccca
     7081 cgctcagccc ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa cgcgcctgat
     7141 cttgcacatc agccgggatc tcaccggtct gtgggcaaac cggaatttga ccggaggtga
     7201 agaccatgct gcctaaatca acgccttgaa cataagggcc gattgcgcct ggggcacgtt
     7261 gcgtttcgat aatctttttc atacatcctc cggcgtcaga gcgcctgggt aaaggtacgt
     7321 gaaataacat cctgctgctg ttcacgggtc agtgcgttga agcgcacggc gtagccagag
     7381 acacggattg tcaggttagg atatttttcc gggtgctcga tggcatccag cagcatttcc
     7441 cgattcatta cgttgacgtt gaggtgttga ccgccttcga catccgcttc gtggtggaaa
     7501 tacccatcca gcaggccgac aaggttggtt ttacgtactg gatcttcttt gcccagcgcc
     7561 gcagggacga ttgagaaggt gtacgagatc ccgtctttag cgtaggtgaa cggcagtttc
     7621 gccaccgacg tcaatgaggc cacggcacct ttgcggtcac gaccatgcat cgggttagcg
     7681 cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg tattaccggt tttctggccg
     7741 tacaccacgt tagaagtgat agtcagaatc gactgggtag ggacggcgtt gcgataggtt
     7801 ggcagcgctt taattttctt cataaaacgt tcaaccaggt cgcaggcaat gctgtctacg
     7861 cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc caccgccagg
     7921 ccgttttcgt cacggattgg tttcacgcgg gcatatttga tcgcagacag ggagtccgtt
     7981 gccaccgaca ggcccgcgat gccgcatgcc atggtgcgat agacatcacg atcgtgcagc
     8041 gccatcagcg aagcttcgta gctgtacttg tcgtgcatat agtgaatgat attcagcgca
     8101 ctgatgtact gcaccgccag ccagtccatg aagtgatcga ggctgtccat cactttgtcg
     8161 taatccagca catcgtccat caacggtgct gttttcggcc cgacctgaat cttcagcttc
     8221 tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg ccaggttagc gcgtgcacca
     8281 aagaactgca tttgcttacc aatcaccatt gggctgacgc agcaggcaat cgcgtaatcg
     8341 tcgctgttga agtcagtacg catcagatcg tcgttttcat attgcaagga agaggtgacg
     8401 atcgacacct gcgcggcata ttttttgaag gcaatcggta attcttccga ccaaagaatg
     8461 gtcaggttag gttccggtgc aggccccata gtgtgcaggg tgtgcaaata gcggaaggag
     8521 tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc cgtcgcccag
     8581 attgggtcgc cggagaacag cgaatcaaat tccggtgtac gcaggaagcg caccatacgg
     8641 atcttcatga tgaagtgatc gatcagttcc tgtgcctgct gctcattgag tacgccagct
     8701 ttaaagtcgc gctcaatgta gatatccagg aacgatgccg tgcgacccag cgacatcgcg
     8761 ccgccgttct gtgatttcac tgccgccaga taagcgaagt agagccactg caccgcttcc
     8821 tgcgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat ttcctgaatc
     8881 tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt ggcttccaga
     8941 tcctcgcctt tttccagacg agactggaga tcggcaaatt gcagttcgcg ttcacgcacc
     9001 agataacgaa tgccatacag cgctacgcgg cgatagtcac cgataatgcg cccacgtcca
     9061 taaccatctg gtaaaccggt cagtacgcca gatttacggc agcgcagcat atccggtgag
     9121 taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag atattcaaat
     9181 tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt aatgccaccg
     9241 aacgggtgta gcgcacgttt caacggcgca tccgtttgca ggccaacaat tttttccagc
     9301 ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa
     9361 tcaaccggcg cgtgggttgc gttttcgata cggatgcctt ccatcacctt ttcccacaat
     9421 tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat caccttcata cggtgtatag
     9481 ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc agtccgtacc tttaaagcca
     9541 agccatgcgt cggcgtacag cttatcgctg gtatcaatat ctaccttcat gaaaaataat
     9601 ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac tttgcctaaa
     9661 tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacggc gcaaatgaca
     9721 cgcgcatttt cactggaaac aattcgctcg ccaaaagagt tagatcgatt attcatttct
     9781 gtatcaatct ctacgcctaa tacagccaaa tgttccatga ccagacgacg aattaaactt
     9841 gaattctctc ctattccgcc ggtgaatata attccatcca ggcgacgtaa tgaagctgcg
     9901 tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag ctgcgcgcgt
     9961 tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag accggaaata
    10021 cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct ctggttggtt
    10081 tggctggcga cccaggacat cgcaccaaag tcgacatcgc cactgcgggt acccatcatc
    10141 aagccttcca gcggcgtcat tcccattgag gtatcaacac tctgaccgtt gcgaaccgcg
    10201 cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc ttccgccaga
    10261 ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg gaaaccataa
    10321 cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata agcttccgga
    10381 gccatcgtct ggtggaaact ggtatcaaat accgccacct gagttacgcc cggaaataat
    10441 tgctgcgccg attcaatacc acttaaattg gcgtaattat gcaggggtgc cagtggagaa
    10501 acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt aaaaatactg
    10561 ccgccgtgag cgatgcggtg gccaattaag gccacgctgt catttaaatt ccgtttttcc
    10621 agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc
    10681 tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac
    10741 attaatactt cacagtcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag
    10801 ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta
    10861 aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt
    10921 atccagacga ccacggtatt tcgccagaga cggcgcttta cggatggcat acatcggcaa
    10981 caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt cgaggatgtt
    11041 cgggttggcg taggcaacaa cccaagtgga gcccatgatg aagatcatgc tgagagtatt
    11101 cagtttaccc agcgacactt tggttttgtc acctttataa ccgaacttca gaatcagacc
    11161 attcaagcct tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag
    11221 tgcgatgatg gaagccgcat attccagtgt aattgcgaac gttgttttcg taccggtcat
    11281 ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc
    11341 catgttggcc ggagacagcg taaacaggca gctaaaggca aagaacatca ccactgcaac
    11401 catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc
    11461 gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa
    11521 gttaaaggag aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg
    11581 accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg
    11641 gatcagcgac aaagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcat
    11701 cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca gcaacaggaa
    11761 cagcgccaca aagccgcgat tcagcggtgc aaagccgagc tggttttccc agaacgtcat
    11821 aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg cgaagaagta
    11881 caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg tttccgtaat
    11941 gttgccggaa ggattagagc cagaaagaca cagacgcgcc agcgcccggt ggcaataaaa
    12001 cgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc caaaacctgc
    12061 gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc ctaacgtcca
    12121 tgtggtatct gatttacgcc aggacgattg ttttgtctgg ctggatacaa tgctatctga
    12181 agtactcata tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg tgatttgaga
    12241 gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc tgttttgaat
    12301 atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg cgccttcggt
    12361 gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt cttcgctgac
    12421 cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac ccgggcgggc
    12481 gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt ctccggagtg
    12541 gaaagaagcc gccatgccgt gaacgttttc tgactgtacg ccaataacac gaatggtcgg
    12601 gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccgccaccac caattggcac
    12661 aatcacgtta tcgacatcat agagatcttc cataatttcc aggccaatcg ttccctgacc
    12721 agcaatcact ttcggatcat cgtaaggtgg gataaaaata cggccttcca tttcgacaat
    12781 ttcgctcact ttagcgatag tgtcgttgaa gttatcacca tgcagaacga cttctgcgga
    12841 gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca ccactttacc
    12901 gtcgataccc agcatcgcgc aggagaggga aaccccttgc gcatggttgc ccgcagaaca
    12961 ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa cttaatttat taaatgcgcc
    13021 acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata tttcaccttt
    13081 gcaacgttca ctaaaatagt tggagcgagg catacctgtt ttataaattc gcccagccag
    13141 tcgttgtttc gcttcaataa tgtcatcaat agcaaccggc agatcgtatg taatatgcat
    13201 tataaaacct cttcgcctga attaaatatg taggtaaccg taaccgacac ctgcaagaca
    13261 gacaggtgga ttatttatgt ttaaataaaa acaaataact aaccaacttc tattaattgc
    13321 cttcgtctac acccattata agatgaatac tctttggcta attccaccaa aaccgatgct
    13381 gcttttttaa tacgataatt tttcgaccat accgcggcat attgtgccac aggtaatgtt
    13441 tcttcaaccg gaatagtaat aaattgatta gaaccaaaag gtgacgtcat atcacaagga
    13501 attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac tgagtcggtt
    13561 ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagcag ttcgctgtag
    13621 taccccatat tggtttgtgg caacacccac tgttcgttct tcaacgactc cagcgtggtg
    13681 gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc gaacagcggc
    13741 tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc aaaatccagt
    13801 cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat agaaacctgc
    13861 gcttttggga acacctcttt gaacttattg atcatccctg acataaaagt aaaaccaatc
    13921 aatgaaggaa aaccaaatga gacttccacc accgcctcag aagacatacc gcttatctca
    13981 ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa caattgaccg
    14041 gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc aaaataatct
    14101 tcaatatcgt taatgatttt actgacggct ggttgagtta accctaattc ttttgcagcc
    14161 gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg cgttttcgga
    14221 agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat gaagtgtatc
    14281 aaaatgaaat gaacaggata tgtgcgacc
//
LOCUS       DAZGBC010000127        14260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGBC010000127 DAZGBC010000000
VERSION     DAZGBC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.128"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            178..1749
                     /gene="garD"
                     /locus_tag="ACHK1J_003660"
     CDS             178..1749
                     /gene="garD"
                     /locus_tag="ACHK1J_003660"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO7912880.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1898..2233
                     /gene="prlF"
                     /locus_tag="ACHK1J_003661"
     CDS             1898..2233
                     /gene="prlF"
                     /locus_tag="ACHK1J_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO7912881.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2233..2697
                     /gene="yhaV"
                     /locus_tag="ACHK1J_003662"
     CDS             2233..2697
                     /gene="yhaV"
                     /locus_tag="ACHK1J_003662"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO7912882.1"
                     /translation="MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2752..3561)
                     /gene="agaR"
                     /locus_tag="ACHK1J_003663"
     CDS             complement(2752..3561)
                     /gene="agaR"
                     /locus_tag="ACHK1J_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO7912883.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRLQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3810..5090
                     /gene="kbaZ"
                     /locus_tag="ACHK1J_003664"
     CDS             3810..5090
                     /gene="kbaZ"
                     /locus_tag="ACHK1J_003664"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO7912884.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPV
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5113..5586
                     /gene="agaV"
                     /locus_tag="ACHK1J_003665"
     CDS             5113..5586
                     /gene="agaV"
                     /locus_tag="ACHK1J_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO7912885.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5597..6376
                     /gene="agaW"
                     /locus_tag="ACHK1J_003666"
     CDS             5597..6376
                     /gene="agaW"
                     /locus_tag="ACHK1J_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO7912886.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6366..7244
                     /gene="agaE"
                     /locus_tag="ACHK1J_003667"
     CDS             6366..7244
                     /gene="agaE"
                     /locus_tag="ACHK1J_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO7912887.1"
                     /translation="MASNQTTLPNISENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTMTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7262..7696
                     /gene="agaF"
                     /locus_tag="ACHK1J_003668"
     CDS             7262..7696
                     /gene="agaF"
                     /locus_tag="ACHK1J_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO7912888.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7693..8826
                     /gene="nagA"
                     /locus_tag="ACHK1J_003669"
     CDS             7693..8826
                     /gene="nagA"
                     /locus_tag="ACHK1J_003669"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7912889.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRYLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQGQLASF"
     gene            9177..10331
                     /locus_tag="ACHK1J_003670"
     CDS             9177..10331
                     /locus_tag="ACHK1J_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO7912890.1"
                     /translation="MPENYTPAAAASGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10344..11204
                     /gene="kbaY"
                     /locus_tag="ACHK1J_003671"
     CDS             10344..11204
                     /gene="kbaY"
                     /locus_tag="ACHK1J_003671"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO7912891.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11371..11847
                     /gene="agaB"
                     /locus_tag="ACHK1J_003672"
     CDS             11371..11847
                     /gene="agaB"
                     /locus_tag="ACHK1J_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO7912892.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11886..12689
                     /gene="agaC"
                     /locus_tag="ACHK1J_003673"
     CDS             11886..12689
                     /gene="agaC"
                     /locus_tag="ACHK1J_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO7912893.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12679..13470
                     /gene="agaD"
                     /locus_tag="ACHK1J_003674"
     CDS             12679..13470
                     /gene="agaD"
                     /locus_tag="ACHK1J_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO7912894.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13471..14226
                     /locus_tag="ACHK1J_003675"
     CDS             13471..14226
                     /locus_tag="ACHK1J_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO7912895.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQHVDVSQLTVVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTSIPASFLWLHSNFICLINT"
BASE COUNT         3446 a         3603 c         3722 g         3489 t
ORIGIN      
        1 aaagcacaga tttgcgcttt accttgccgg gcggcattgc aatccctgaa atgattgaca
       61 ttgatcacat ttctgcattt aaactcctga cattcttatt tcacccaatg aagtcattta
      121 tttttaaatg agaccaggtc ctcattttaa taacccctgg ctggagaata ttgcacaatg
      181 gccaacatcg aaatcagaca agaaacgcca actgcgtttt atataaaagt tcacgacaca
      241 gataatgtgg caattattgt taatgataat ggcctgaaag cgggaacgcg ttttccggat
      301 gggctggaat taattgaaca tattccccag gggcataaag tcgcattgct ggacattccg
      361 gctaatggtg aaattattcg ttatggcgaa gtgattggtt acgccgtgcg tgcaatccca
      421 cgcggaagct ggatcgacga atcaatggtt gtactaccgg aagcgccgcc gttacacacg
      481 ctgccactgg caaccaaagt cccggaaccc ttaccgccgc tggaaggata cacctttgag
      541 ggctatcgca atgccgatgg cagcgtgggc accaaaaacc tgctcggcat caccaccagc
      601 gtccactgtg tggcaggcgt ggtggactac gtagtaaaaa tcattgaacg cgatctgctg
      661 ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc atctgtacgg ttgtggcgtg
      721 gcgattaacg ctccggcggc agttgtacct atccgtacca ttcacaatat ttcgctgaat
      781 cctaactttg gcggcgaagt aatggtgatt ggcctgggtt gtgaaaagtt gcagcctgag
      841 cgcctgctga ctggaacgga tgatgtgcaa gctattccag tagaaagcgc cagcattgtc
      901 agtttgcagg atgaaaagca tgtcggtttt cagtccatgg tcgaggatat tttgcaggtc
      961 gccgaacgcc atttacaaaa actgaatcaa cgtcagcgag aaacctgtcc ggcttcagaa
     1021 ctggttgtcg gcatgcagtg cggtggcagc gatgcctttt ctggcgtaac ggcaaacccg
     1081 gcggttgggt atgcgtctga tctactggtg cgctgcggcg caacggtgat gttctcagaa
     1141 gtcacggaag tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc
     1201 ggcaaacggc tgctggagga gatggagtgg tacgataact atctcaatat gggaaaaacc
     1261 gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg gtctggcaaa tgtagtggag
     1321 aaagcgctcg gctccattgc taaatcgggt aaaagtgcaa ttgttgaagt gctgtcgccc
     1381 ggtcaacgcc cgactaaacg cggattaatt tacgccgcga cgccagccag cgattttgtc
     1441 tgtggcacgc aacaggtggc ttcgggtatc accgtgcaag tgtttacgac cggtcgtggt
     1501 acgccgtacg gcctgatggc ggtacccgtc attaaaatgg cgacccgcac cgagctggcg
     1561 aaccgctggt ttgatttaat ggatattaac gcgggcacta tcgccaccgg cgaagaaacc
     1621 attgaagagg tgggctggaa gttgttccac tttattctcg acgtcgccag cgggaagaag
     1681 aaaaccttct cggatcaatg gggactgcat aaccagctgg cggtgtttaa cccagcaccg
     1741 gtgacctgat ttcttttgga acgtccctcg caaaacatgg ccttagtgcc atgtttttat
     1801 tgtttaaagc ccccacgtcc attaataatg catttgcatt accttagttc aagcttataa
     1861 tttgagcaga aaacaggctg taaaaggaca gtgaatcatg cccgctaatg ctcgctctca
     1921 cgctgtactg accactgaat caaaggtcac gatacgcgga caaacaacta tccccgcgcc
     1981 agtgcgtgag gccttaaaac tgaagccagg ccaggacagc attcattacg aaattctgcc
     2041 tggtgggcaa gtatttatgt gccgactggg agatgaacag gaggatcata ctatgaatgc
     2101 atttttgcgt tttctggatg cagatatcca gaacaacccg caaaaaactc gtccattcaa
     2161 cattcaacaa ggaaagaaac ttgtcgctgg catggacgtc aacattgatg atgagattgg
     2221 cgacgacgaa taatggattt tccacaaagg gttaatggtt gggcgctata tactcatccc
     2281 tgttttcagg aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat
     2341 cctgaaaatt atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt
     2401 gaggagcata tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc
     2461 tctgggaaaa ataaagactg gtcacgggta aaatttggtg ctggtcgtta tcgtctcttc
     2521 tttcgttata gtgaaaaaga gaaagtcatc attctgggat ggatgaacga tgaaaacacg
     2581 ctgcgcacct acggtaaaaa aacagatgcc tataccgtat tcagcaaaat gttaaaaaga
     2641 ggacatcctc ctgccgactg ggaaaccctc acccgagaga cagaagaaac ccattgatgg
     2701 tgttcacaat gcccgccgaa cgcattcgtg ttgaccagcg ggcacccttt ctcactcccc
     2761 gaccagaatc acttcaaccc cagcctttcg cagtccttcc agactatccg caggaatgcc
     2821 ttcatcaaca atgatcatgt cgatacgttg agtatcaatg atcttatgta aactggagcg
     2881 gttgaactta ctggaatcgg tgactacgat gatccgttcc gcaacttcgc acatccggcg
     2941 gtttaaacgg gcttcatctt cattatgcgt gctgacgccg cgctccagat caatcgcatc
     3001 tacaccaaga aacagcatat cgaagtggta attttgcagc gattgctcag cctgatcgcc
     3061 gtaaaaagat tgcgactgac ggcgcaaatg cccgccggtc atcagcagct caacgccttc
     3121 cgcttccagc aaagcattag ccacgttcat accgttggtc atcgcaatta cgtcagtgtg
     3181 cttgcgcatc agacgagcaa tctcaaaagt ggtggtcccg gaatcgagga tcacccgatg
     3241 acctggctga atcaactcaa cggcagcttt cgcaacgctg cgtttcattg cggtgttcag
     3301 cgcgctttta tcttccactg atggctcgac tgacggcgtt gtgctatcgc agatcaacgc
     3361 gccaccatag gcacgcacag cgatcccctg cttttccaga aacgccagat cgttgcggat
     3421 cgtcacagta gatacgccat acaatgccga cagatcgtta acctgcacac tcccttgcag
     3481 tcgcagacgc tgaatgatct gttctcgtcg ctcgctggtg cctgtcactc gcttctcacc
     3541 tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa ctttcgtttc atttcgtttt
     3601 gcctattaac gcctttctat taagcaaatg caagcccacc ttgcccattg acgcaagcta
     3661 ctctcgtttc agtgactttc attatgtttc ttttgtgaat cagatcagaa aaccattatc
     3721 tttcgtttta tttttatctc accatgacgc agtatcaact gaaacaaaac gaaagattaa
     3781 tatcgcagta atctgaactg gagaggaaag tgaaacatct gacagaaatg gtgagacagc
     3841 acaaagcggg caaaacaaat ggaatttatg ccgtttgttc cgcacatccg ctggtgctgg
     3901 aggctgcaat ccgctacgcc agtgcaaacc aaacgccgtt actgattgaa gcaacctcca
     3961 atcaggtaga ccagttcggc ggttataccg gaatgacgcc cgccgatttt cgcggctttg
     4021 tttgtcagct cgccgactcg ttgaatttcc cgcaggatgc gttgattctg ggtggtgacc
     4081 atctggggcc aaaccgctgg caaaacctgc cagtcgctca ggcaatggcc aatgccgatg
     4141 atttgattaa aagctacgtt gcggcaggat tcaaaaaaat ccaccttgat tgcagcatgt
     4201 cctgtcagga cgatccaatt cccttaactg atgacatcgt ggctgaacgc gccgcccgtc
     4261 tggcgaaagt ggcggaagaa acctgtcttg aacactttgg cgaagccgat ctggagtatg
     4321 tcattggtac cgaagtgccg gtacctggcg gcgcgcatga aaccttaagc gagctggcgg
     4381 tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca tcgtcacgcc tttgaaaagc
     4441 aaggtttgaa tgccatctgg ccacgcatca ttgccctggt ggttcaaccc ggcgtcgaat
     4501 tcgatcacac caacgttatt gattatcagc ccgccaaagc gaccgcctta agccagatgg
     4561 tcgaaaacta cgaaacgctg attttcgaag cgcactctac cgattatcaa acgccgcaat
     4621 cgctgcgcca gctggtgatt gaccactttg ccattctgaa agttggccca gcgctgacct
     4681 tcgccctgcg tgaagctctg ttctctctgg cagcgattga agaagaactg gtgccagcaa
     4741 aagcctgttc tggtctgcgt caggtgctgg aaaacgtgat gctcgaccgc ccggaatact
     4801 ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct ggcgcgtggt tatagctact
     4861 cggatcgcgt gcgctattac tggccggaca gccagattga tgacgctttc gctcatctgg
     4921 ttcgtaatct ggcggattca ccaattccgc tgccgctgat cagccagtat ctgccgctgc
     4981 agtacgtgaa agttcgctcc ggcgagctgc agccaacgcc acgggaactc attatcaacc
     5041 atattcagga catcctggcg cagtaccaca cagcctgtga aggccaataa gcaaaacaaa
     5101 gaggaacacg ctatgccaaa tattgtttta agccggattg atgaacgctt gattcacggt
     5161 caggtcggcg ttcagtgggt cggatttgcg ggggcaaatc tggtgctggt cgccaacgat
     5221 gaggttgccg aagatccggt acaacaaaac ctgatggaaa tggtactggc agaagggatc
     5281 gccgtacgtt tctggacgct gcaaaaagtt atcgacaaca ttcatcgcgc cgccgatcga
     5341 cagaaaatcc tgctggtttg taaaacaccc gccgatttcc tgacgctggt gaaaggtggc
     5401 gttccggtga atcgcattaa cgttggcaat atgcactacg ccaatggcaa gcaacaaatc
     5461 gccaaaacgg tttctgtgga tgcgggcgat atcgcagcat ttaacgacct gaaagccgct
     5521 ggggtggaat gcttcgttca gggcgtcccg acagagcctg ctgtggacct ctttaaatta
     5581 ctttgaggga ttcatcatgg aaatcagtct gttgcaggca ttcgcgttgg gcattatcgc
     5641 ctttatcgct ggcctggata tgtttaacgg cttaacccat atgcaccgcc cggtggtcct
     5701 cggcccgtta gtcgggctgg tacttggcga tctgcatacc ggtattttaa ccggcggtac
     5761 gctggaactg gtgtggatgg ggctggcccc gctggcgggc gcacagccgc ctaacgtgat
     5821 tatcggtact atcgtcggca cggcgtttgc cattactact ggcgtgaaac ccgatgtcgc
     5881 agtcggtgtc gccgtacctt tcgctgtcgc agtacagatg gggattacct tcctgttctc
     5941 ggtgatgtct ggcgtgatgt ctcgctgcga ccggatggcg gagaacgccg atactcgcgg
     6001 cattgaacgc gtaaactatc tggcactgct ggcactcggc actttctatt ttctctgcgc
     6061 tttcctgcct atctacttcg gcgcggaaca tgcaaaaacc atcattgatg tcctaccgca
     6121 acgattaatc gacggcctcg gcgtcgcggg cggcatcatg ccagcaatcg gttttgccgt
     6181 gctgctgaaa atcatgatga aaaacgtcta catcccctac ttcatcctgg gctttgttgc
     6241 cgcagcctgg ctcaagttac cggtgctggc tattgctgcc gccgcgctgg cgatggcgct
     6301 gatcgatctg ctgcgtaaat ctcctgaacc gactcaacct gcggcacaga aagaggaatt
     6361 tgaagatggc atctaatcaa accaccctgc cgaacatcag tgaaaacgaa gaaacattgc
     6421 tgactggcgt caatgaaaac gtgtatgaag atcagagcat tggcgcggag ctgacgaaaa
     6481 aagatatcaa tcgcgtcgcc tggcgttcca tgctgttaca agcgtctttt aactacgaac
     6541 gtatgcaggc ttccggctgg ttgtacggtc tgctgcctgc tctgaaaaag atccacacta
     6601 ataaacgcga cctggcgcgc gccatgaagg ggcatatggg tttcttcaat acccatccgt
     6661 ttctggtgac atttgttatc ggcattatcc ttgcgatgga gcgttctaag caggacgtta
     6721 acagtattca gagcaccaaa attgccgtcg gtgcgccgct cggcgggatt ggcgatgcaa
     6781 tgttctggct aacgctactg ccgatttgtg gcgggattgg agccagtctg gcattgcaag
     6841 gctccattct tggcgcagtc gtctttattg tgctgttcaa cgtggtacac ctggggctgc
     6901 gttttggtct ggcgcattat gcttaccgca tgggcgtggc ggcgattcca ctcattaaag
     6961 caaataccaa aaaagtcggc catgctgcgt ctatcgttgg gatgacggta atcggtgcgc
     7021 tggtggcaac ctatgttcgt ttaagcacca cgctggaaat cacagcgggc gacgcagtgg
     7081 ttaagttaca ggctgatgtt atcgacaaac tgatgcctgc cttcttaccg ttggtctaca
     7141 ccatgaccat gttttggttg gtacgccgcg gctggagtcc gctgcgtctg attgcagtta
     7201 ccgtggttct cggcatcgtc ggtaaattct gccatttcct ttaaatacaa tgaggtatta
     7261 gatgttaagt attattttga cagggcatgg cgggtttgcc agcggcatgg aaaaagcaat
     7321 gaagcaaatt ctcggtgagc aatcgcagtt tatcgccatc gattttccgg aaacctcaag
     7381 caccgcgctg cttacatcgc agcttgaaga ggccatcgcc caactggatt gtgaagacgg
     7441 cattgttttt ctgacggatt tgctgggcgg cacgccgttt cgcgtagctt caacactggc
     7501 gatgcaaaaa ccgggctgtg aagtgatcac cggcaccaat ttgcaactgc tactggagat
     7561 ggtgctggag cgcgaagggt taagcggtga agaattccgt gtgcaggcgc tggagtgtgg
     7621 acatcgtggg ctgaccagtc tggtggacga gttaggccgc tgccatgagg aatgtccggt
     7681 cgaggaagga atatgacaca cgttctgcgc gccagaaggc tgctgactga agagggatgg
     7741 ctcgatgacc atcagttgcg tattgctgac ggtgtcatcg cagcaatcga accgattcca
     7801 gtgggcgtga ctgaacgcga tgcggaactg ctctgccctg cttacatcga cacccatgta
     7861 cacggtggtg cgggcgttga tgtaatggat gacgcgccgg atgtactcga caagctggca
     7921 atgcacaagg cacgcgaagg tgtcggcagt tggttaccga ccaccgtaac cgcgccgctt
     7981 aataccattc atgcggcgct gaaacgtatt gctcaacgtt gccaacgcgg cggacctggt
     8041 gcgcaagtgc tggggagtta tctcgaagga ccgtacttca cgccgcagaa taaaggcgcg
     8101 catccgccgg aattgtttcg cgagcttgaa attgccgagc tggatcaatt gattgccgtt
     8161 tctcagcaca ccttacgcgt ggtagcgctg gcaccggaaa aagaaggggc attgcaggcc
     8221 atccgctatc ttaaacagca aaatgtaagg gtgatgctgg ggcatagcgc ggcgacctgg
     8281 caacaaaccc gcgccgcgtt tgatgccggt gccgacggcc tggtgcattg ctataacggg
     8341 atgacaggtt tacatcaccg cgaaccggga atggttggcg cgggattaac ggacaagcgc
     8401 gcctggctgg aactgatagc cgatggtcat catgtgcatc cggcggcaat gtcgctgtgt
     8461 tgttgctgtg cgaaagagag aatcgtgctg atcaccgacg cgatgcaggc agccgggatg
     8521 ccggatggtc gctatacgtt atgtggtgaa gaagtgcaga tgcacggtgg cgttgtccgt
     8581 accgcgtccg gtgggctggc gggcagtacg ctgtctgttg atgcggcagt gcgcaatatg
     8641 gtcgagttga cgggcgtaac gcctgcggaa gccattcata tggcatcgct gcatccggcg
     8701 cgaatgctgg gtgttgatgg tgttctggga tcgcttaaac cgggcaaacg cgccagcatc
     8761 gttgcgctgg atagcgggct gcatgtgcaa caaatctgga ttcagggtca attagcttcg
     8821 ttttgatggt ttgctccttt attgggcctt cacttccccc gtaaggcctt tctttttctt
     8881 tcgttttgat ctgtgcagcg gtgtcggatg cgacgctaac gcgtcttatg cgacctacgg
     8941 tacgccgcat ccggcaatcg gtgaacatag cgacgtcatt acgcaattct ttaatcttcc
     9001 cttgtctttt ctttcaatga tcacagattt cgttttattt cctttttctc cattgaactt
     9061 tcggtttctt ttctatagat tttaatcaac gaaagacatc accaagtgaa atgaaacgaa
     9121 aggcaagtga aagcgacaac gcccgacgtc aagttcatca gactaaggat tgagttatgc
     9181 cagaaaatta cacccctgct gccgccgcat ccggtacatg gactgaagaa gagatccgcc
     9241 atcagcctcg cgcatggatc cgttcactca ccaacatcga cgcgctacgt tccgcgctca
     9301 ataacttcct tgaaccgtta ctgcgcaaag agaatctgcg gatcatcctg accggagccg
     9361 gaacctcggc atttatcggt gacatcatcg cgccgtggct cgccagccat accggtaaaa
     9421 acttcagcgc cgtaccgacc accgatctgg tcactaatcc gatggactac ctgaacccag
     9481 cccatccgct gctgttgatc tccttcggtc gatccggcaa cagcccggaa agcgtcgcag
     9541 ccgtggaact ggcaaatcaa tttgtaccgg aatgctatca cctgccgatc acctgcaacg
     9601 aagcgggcgc tctttaccaa aacgcgatca acagcgataa cgcgtttgcc gtgctgatgc
     9661 ccgcagaaac gcacgatcgc ggctttgcga tgaccagcag cattaccacc atgatggcca
     9721 gctgcctcgc ggttttcgca cctgagacga tcaacagcca aaccttccgc gacgtggcgg
     9781 atcgttgcca ggcgatcctg acctcactgg gcgatttcag cgaaggtgtg tttggttacg
     9841 caccgtggaa acggatcgtt tatctcggca gcggtggctt acagggcgca gcacgcgagt
     9901 cggcgctgaa agtgctggaa ctgacggcgg gtaaactggc ggccttttat gattctccaa
     9961 ccggattccg tcatggacca aaatcgctgg tcgatgacga aacgctggtg gtggtatttg
    10021 tctccagcca cccttacacc cgtcagtatg atcttgatct gctggctgaa cttcgccgtg
    10081 acaaccaggc aatgcgtgta atcgccatcg ccgcggaaag cagcgacatc gtcgctgccg
    10141 gtccacatat catcctgcca ccgtcacgtc actttatcga cgttgagcag gcattttgct
    10201 tcctgatgta cgcccagacg tttgcactga tgcagtcgct gcacatgggc aatacgccgg
    10261 ataccccatc agccagtggc accgttaacc gcgtggtgca aggcgtaatc attcatccgt
    10321 ggcaggcata agaggatcgc attatgagca ttatctccac taaatatctg ttacaggacg
    10381 cccaggccaa tggctacgcg gtgcctgcct ttaacattca taacgccgag acgatccagg
    10441 cgatcctcga agtgtgcagt gaaatgcgat cgccggtgat cctcgccgga acgccgggga
    10501 cctttaaaca tatcgcgctg gaagagatct acgccctgtg cagcgcctat tccacaacct
    10561 acaacatgcc actggcgctg catctcgacc accacgaatc gctggatgat attcgccgta
    10621 aagtccacgc aggtgtgcgc agtgcaatga tcgacggcag ccacttcccg tttgccgaga
    10681 acgtgaagct ggtgaaatcg gttgttgact tctgccactc acaggattgc agcgtggaag
    10741 cagaactggg ccgcctgggc ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat
    10801 tcctgaccga tccacaagaa gctaaacgct ttgtcgaact gactggcgtc gacagcctgg
    10861 cggtagcgat tggtacggcg cacggcttat acagcaaaac gccgaagatt gatttccagc
    10921 ggctggcgga aattcgtgaa gtggtggatg ttcctctggt gctacatggt gccagcgatg
    10981 ttccggatga atttgtccgt cgcactattg aacttggcgt cacaaaagtg aacgttgcca
    11041 cagaattaaa aatagccttc gccggcgcgg ttaaagcctg gtttgcggaa aatccgcagg
    11101 gtaatgatcc tcgttattat atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa
    11161 ataaaattaa tgtctgtggt tcagcgaatc gaatttcagc ataatcatta gttttttatg
    11221 atttatccca atgtacttcc cgattaatat cggggagtgc cttaatggaa aaggagatga
    11281 ctaaacctta ataaatacat cagtacaata tcgcaacaat aatatattta aaaacattat
    11341 attattcaac tttatggtga ggattacaca atgagcagtc caaatattct cttaacccgt
    11401 attgataacc gtctggttca cggtcaggtt ggcgtgacct ggacatccac catcggtgca
    11461 aatctgctgg tagtcgtgga tgatgttgtc gctaacgatg atattcaaca gaaattaatg
    11521 ggtattaccg cagaaaccta cggctttggc attcgtttct ttactatcga aaaaaccatt
    11581 aacgtcatcg gcaaagctgc accgcatcag aagatcttcc tgatttgccg tacgccacaa
    11641 acggtacgta aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg
    11701 catttctcgg aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc
    11761 acggacttac gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc
    11821 gatcaaaaag aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa
    11881 taaaaatgca tgaaataacc ctacttcagg gattatccct ggcggcgtta gttttcgttc
    11941 tggggattga tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc
    12001 taactggcgc tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt
    12061 tggctttcgc cggattaacc cctgcaggtg gtgttcagcc gcccaaccct attatggcgg
    12121 gtctgatgac caccgtcatt gcatggtcta cgggcgttga tgccaaaacg gcaattggtc
    12181 ttggcctacc gtttagtttg ttaatgcagt acgtcattct gttcttctat tctgctttct
    12241 cattatttat gaccaaagcc gataaatgcg cgaaagaagc ggatacggca gcgttttccc
    12301 gacttaactg gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct
    12361 gtacttacct ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggctga
    12421 cccacggctt tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc
    12481 gcgtgatgtt caaagcacaa tatatccctt acctgatcgc cggcttcctg tttgtttgct
    12541 acatccaggt cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg
    12601 agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg
    12661 aagaagagga ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc
    12721 tttcgttcgt cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt
    12781 acctgggcga tgttgccgat cctgaaaaag atttataagg atgacaaacc gggcctaagc
    12841 gcagcgatga aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg
    12901 atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc
    12961 aaagtggcac tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg
    13021 ttgccgatta tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg
    13081 attctatttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc
    13141 ggttattcag tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct
    13201 cgctcggcaa ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat
    13261 attaacgtgg tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat
    13321 ttcttcgata aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac
    13381 ttcctgcggg tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct
    13441 attgtttgtt ccgcattcgg cattttgtaa atggaacgag gcactgcgtc tggcggtgcc
    13501 tctttactta aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg
    13561 ttaagtgaac gtgccagcga atatttattg gccgtgatcc gtagcaaacc ggatgccgtg
    13621 atttgcctgg cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc
    13681 caccagcagc acgttgatgt cagccagctc accgtcgtca aactcgatga atgggtggat
    13741 ctgccattaa cgatgcctgg tacctgcgaa actttcctgc aacagcatat cgtgcagccg
    13801 ctggggctac gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag
    13861 tgcgaacggg taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga
    13921 ttggggaaaa acggtcatct tgggctgaac gaaccggggg aaagcctaca accggcctgc
    13981 catatcagtc aacttgatgc cagaacacag caacatgaga tgttaaaaac cgcgggtcgc
    14041 cccgtgactc gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg
    14101 ttactggtga ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc
    14161 tctacctcta tcccggcttc gtttttgtgg ctgcacagta actttatttg tttaattaat
    14221 acctaaccag atgaaaaatt tgctcaaaat gtttaaatta
//
LOCUS       DAZGBC010000128        13920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGBC010000128 DAZGBC010000000
VERSION     DAZGBC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.129"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..719)
                     /gene="pgpA"
                     /locus_tag="ACHK1J_003676"
     CDS             complement(204..719)
                     /gene="pgpA"
                     /locus_tag="ACHK1J_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO7912896.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(697..1674)
                     /gene="thiL"
                     /locus_tag="ACHK1J_003677"
     CDS             complement(697..1674)
                     /gene="thiL"
                     /locus_tag="ACHK1J_003677"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO7912897.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1752..2171)
                     /gene="nusB"
                     /locus_tag="ACHK1J_003678"
     CDS             complement(1752..2171)
                     /gene="nusB"
                     /locus_tag="ACHK1J_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO7912898.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2191..2661)
                     /gene="ribE"
                     /locus_tag="ACHK1J_003679"
     CDS             complement(2191..2661)
                     /gene="ribE"
                     /locus_tag="ACHK1J_003679"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO7912899.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2750..3853)
                     /gene="ribD"
                     /locus_tag="ACHK1J_003680"
     CDS             complement(2750..3853)
                     /gene="ribD"
                     /locus_tag="ACHK1J_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO7912900.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3857..4306)
                     /gene="nrdR"
                     /locus_tag="ACHK1J_003681"
     CDS             complement(3857..4306)
                     /gene="nrdR"
                     /locus_tag="ACHK1J_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO7912901.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4457..4996
                     /locus_tag="ACHK1J_003682"
     CDS             4457..4996
                     /locus_tag="ACHK1J_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO7912902.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHNVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLDFVRIHDIQPVMH"
     gene            5295..6179
                     /gene="tsx"
                     /locus_tag="ACHK1J_003683"
     CDS             5295..6179
                     /gene="tsx"
                     /locus_tag="ACHK1J_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO7912903.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6369..6716)
                     /gene="yajD"
                     /locus_tag="ACHK1J_003684"
     CDS             complement(6369..6716)
                     /gene="yajD"
                     /locus_tag="ACHK1J_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO7912904.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6845..7816)
                     /gene="secF"
                     /locus_tag="ACHK1J_003685"
     CDS             complement(6845..7816)
                     /gene="secF"
                     /locus_tag="ACHK1J_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO7912905.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(7827..9674)
                     /gene="secD"
                     /locus_tag="ACHK1J_003686"
     CDS             complement(7827..9674)
                     /gene="secD"
                     /locus_tag="ACHK1J_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO7912906.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9702..10034)
                     /gene="yajC"
                     /locus_tag="ACHK1J_003687"
     CDS             complement(9702..10034)
                     /gene="yajC"
                     /locus_tag="ACHK1J_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO7912907.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10057..11184)
                     /gene="tgt"
                     /locus_tag="ACHK1J_003688"
     CDS             complement(10057..11184)
                     /gene="tgt"
                     /locus_tag="ACHK1J_003688"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO7912908.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11239..12309)
                     /gene="queA"
                     /locus_tag="ACHK1J_003689"
     CDS             complement(11239..12309)
                     /gene="queA"
                     /locus_tag="ACHK1J_003689"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO7912909.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12402..12983
                     /gene="acpH"
                     /locus_tag="ACHK1J_003690"
     CDS             12402..12983
                     /gene="acpH"
                     /locus_tag="ACHK1J_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO7912910.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVITILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYDALETRFWQFYPRMMAQASRKAL"
     gene            complement(13004..>13920)
                     /locus_tag="ACHK1J_003691"
     CDS             complement(13004..>13920)
                     /locus_tag="ACHK1J_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912911.1"
                     /translation="RTKQLEKKIAKAGFITGKVNAEKFNVKEFKDDITSMLCEQPPMC
                     THQISSSGYLSADKVPDLYKTNLKDAQRDLKTTPVDIDGVSLKSKTREKIAEEIAKKT
                     AEKTAEKTAKKNSEIIAKQNANSIEEKNIEKNNKETNPEDNFVLKFSQEEINEMLLDY
                     SDEEQKKLRPFFTSDHIYCLFDAFSQCLYNVARDGSPRYAGLLQQIASKCAILQDFDQ
                     QLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDFPHINDNGELSRTVLMFEGVAES
                     NFKDIANHSSECFSYRCYAAPLDANAKK"
BASE COUNT         3412 a         3727 c         3357 g         3424 t
ORIGIN      
        1 gcaattatta tgagttcgtc gactgacgat atttcagtgt cattgaatta tgagtgacaa
       61 agatacacct tgaaatatcg ccgcatagta tattcggcga tgtcgggttt tactttaaca
      121 gagtgcccca aaattggggc actgttcatt tataaatcaa accgggtggt ctggaatcgc
      181 ctacacctcc cggtcaatgg catttaaaga atacccagcg gccagtgatg accgataaag
      241 tacaggatgc ctgcggaaat caccccggcg acaatatcat cgatcatgat ccccatgccg
      301 ccatgtacat tgcgatcaaa ccagcggatc ggccacggct tccacatatc cagaatacgg
      361 aaaatcacaa acccggcggc gacccactgc cagtcattgg tcggcagcgc catcagggtg
      421 atccacatgc caataaactc atcccagacg atgctaccgt gatcgtgcac acccatgtct
      481 ttcgccgttt gatgacaaag atagacgccg atacagatcc ccaacatcac caccagcgag
      541 tagagctgcc agggcaaaaa ggtcatcaga taccagaacg gaatcgccgc cagcgatccc
      601 atcgtgccag gaacgattgg gcttaatcca cttccgaatc cgacagcaag cagatgccac
      661 ggattactca tcttcaggcg acttttcgcg acatctttat ggcgtggcaa aatggtcata
      721 tcctttccag tctaacgtga caggttcgcc gtcacgaata aaacaaagcc cttcgatatc
      781 ggcggtcatt tgcccgatac aggtaaacgg tacgcccaga tgaccgagag ccacatccag
      841 cgcgccacgg ttcagttccg gcaccgtgaa acacaactcg taatcttcac cgccagagag
      901 cgcccagcgc agcgcctgtt ccggtacaac atggcgagaa agcgcatcag aaaacggcag
      961 caatgccagg tcaatacgtg cgccgcagtc gctggctttc acgatatgcc cgagatcgga
     1021 aatcagaccg tcagagagat cgatagctga atttgccaga tcgcgcagtg cctgcccctg
     1081 taaaatacgc ggcgatggac ggagatgacg tttgatcaag tagtccgcat ctttagcatc
     1141 ggcaacctgc aaacggtttt gcaaaatcgc cagcccggcg gcgctatcgc ccggtgtacc
     1201 ggtcacatag atccagtcac ccggtttcgc cccagagcgc gttaaggctc gtcccatcgg
     1261 aacaaagccg tggataccca acgtcattga taatggccca cgcgtggtat cgccgccaat
     1321 gagttgcata tcgtaataat tgagaagatc aaacaaactg tcgctgaagg actcaagcca
     1381 cgcttcgtct acgtccggta aggttaatgc cagcgtcagc caggccggat cggcccccat
     1441 cgctgccaga tcgcttaggt tcaccgccag tgctttataa gccagatcag caggatcgat
     1501 atcagggagg aaatggttgc ccgccaccag cgtatcagtg ctgatcgcca gggtctgttt
     1561 ctcggggata ttgagaagag cgcaatcgtc gccgatgccc agttcgacat caagacgaga
     1621 acttcttaca cggtcaaaat aacgggcaat cagggagaac tcgccacatg ccatacgtta
     1681 tgcctcagca ggtaaaaaag aaaaggccgg ggatcgcgga acagtcttcc gtgaatctac
     1741 cggcctggat attacttttt attagggcga atcacaggtg ctgctttatc gagtacgccg
     1801 ttgacgaact tatggctgtc ttctgcgccg aacgatttcg ccagttcgat cgcttcgtta
     1861 atggccactt tgtatggcac atcgctacgt ttagacagtt cgtacagcgc aatgcgcagt
     1921 actgcttttt ctacctgtcc cagttcttcc agcaggcggg acaggtatgg cttcatcagt
     1981 ccgtcgaggt atgcggtatt agtcgccacc ccggccagca gctcacggaa gtacaggacg
     2041 tcaacgtctt ttacatcctg ttcagccagg aactggtatt caacatcagc gatgtcgttc
     2101 tgggacaact gccaggagta gagcgcctgg acggcacact cacgagcgcg gcgacgagca
     2161 gcaggtttca cggatttccc cttactaatt tcaggccttg atggctttca atacattaat
     2221 catttcaagc gcggtcagtg cagcttctgc acctttgttg ccagctttgg tgccagcacg
     2281 ttcgatcgct tgttcaatgc tttcagtggt cagaacccca aaagcaaccg gaatttcgct
     2341 gtcctgggca acatgcgcca ggccgttgct tgcaccacca gcgacatatt caaagtgggc
     2401 agtgccacca cgaataaccg tacccagcgc aatcaccgcg tcgtatttac cggttttagc
     2461 cagtgcaccc gccgccagcg gcagctcata ggcaccaggc acccaaacaa cggtaatgtt
     2521 ttcatctttt acctgaccga tacgtttcag tgcgtcaatt gcaccttcca gcaggctgtc
     2581 attgataaag ttgttgaaac gcgcgatggt gatggcgacg cgagcgtccg gggtagcaac
     2641 gttagcttca ataatgttca ttttcttcct tcgggttcga gtatggcccc gcaggggggc
     2701 ggattttagc ataatatttc gtgcgctgct tccctttcga gccgggagat catgcaccca
     2761 ctaaatgcag gcaaacatcc gggcctacat gacgtatctc tttgaattta aattgggggg
     2821 cgtcggctaa tttctcaagc cctggcagcg agcataatcc acgggcgtcg ctgcctaata
     2881 gtttaggtgc gatatagaca atcagctcat cgactaaacc cgcctgcagc aatgcgccag
     2941 cgagcgttgg ccccgcttcc acccagatgc tgttaatctg ctgtttaccc agttgcatca
     3001 tcagtacaac cagatccaga tgacctttat gctctggaat cagcaaggta cgcaccgttt
     3061 ccggccactc acgagaatct tcctgcgtac gcgcgaacca ggtttcgccg ggctgctgca
     3121 caatgtgatg ttccggcgtc acgcgatttt ggctatcaat cacaatacgt accggctggc
     3181 ggagattttg ttgcgggtag agcgcctgag tttgttcatc cagttcggac caacgcaccg
     3241 ttaaggcagg atcatccgcc agcaccgtgg cgctgctggt taaaatggca tgactttgcg
     3301 cgcgcagtcg ttgtacatca cgccgcgcct ggggcgaagt gatccactgg ctttcgccgc
     3361 tcgccatcgc cgtgcgacca tcaagcgatg cgccaagttt taactgaata taaggaaagc
     3421 cggtgcgcat ccgtttgaga aagcctttat tcaattgctc ggcttcactc atcatcagcc
     3481 cgtggctgac gtcaatgcca gcctgttgca gacggtaaag tccacgtcca gcaacctgcg
     3541 ggttcgggtc ctgcatcgca gcgaccacgc gcgctacgcc agcggcgatg agtgcgtcac
     3601 agcacggtgg cgtacgacca tgatggctac agggttcgag tgtgacatag gcggtcgcac
     3661 ctttggcttt ttcacccgcc atacgcaacg cgtgtacttc agcatgtggt tcacccgctc
     3721 gttggtggta accttcaccg acaatttcgc catctttgac aatgacgcac ccgacattcg
     3781 ggttggggtg ggtggtaaaa cgtcctcgtt gcgccagctt tagcgcccgc gccatgtaat
     3841 actcgtcctg cacggcttag tcctccaggc gcgcgatctc ttcgccaaat tctttgatat
     3901 cttcgaaact gcggtagaca gaggcaaaac ggatataggc gactttatcg agctttttca
     3961 attgctccat caccagattg ccaatcatct tgctcggcac ttcgcgctca ccggtggcgc
     4021 gcagctgcga tttaatatga ttgattgcca tttcgacgtc atcggaactc accggacgtt
     4081 tttccagcgc ccgcagcatt ccgctacgta atttttcttc attaaacggt tcacgcacgt
     4141 cgttgctttt tacaacacgc ggcataacca gctccgccac ttcaaaggtg gtgaaacgtt
     4201 cattacacac cagacactgc cgacggcggc gtacggatga accctcgccc acgagacgag
     4261 agtcaattac cttagtgtcc acggcgaaac agaatgggca atgcatacgg cgtcctgacc
     4321 aggtggttaa cagaaatcta ttttaccctg aactgacgat acaacaaagg cgcaacgctt
     4381 ttgagataaa ggatcgatgc tttcgctgct tttaccctct accattaggc taattgcgac
     4441 gagaaaggga cgacctatga atactaacgt ttttcgactg ctcctgctgg gaagcctgtt
     4501 cagccttagc gcctgtgtgc agcaaagcga agtgcgacag atgaaacaca acgtcagcac
     4561 gctgaaccag gagatgacgc aactcaatca agaaacagtc aaaatcactc agcaaaacag
     4621 gctgaatgca aaatccagca gtggggttta ccttctgcct ggagcgaaaa caccggcaag
     4681 actggaaagc cagatcggta ctttacgtat gtcgctggtg aatattacgc ctgatacaga
     4741 tggcaccacc ctgacgctac gtattcaggg cgagtcgaat gatcccttgc ccgcattcag
     4801 cggaaccgtt gaatatgggc aaattcaggg aacaatagac aactttcagg aaatcaatgt
     4861 gcagaatcaa ttgattaatg cccctgccag cgtcctcgcc cccagcgatg ttgatattcc
     4921 gttacagtta aaggggattt ctgtggatca gttagacttt gtacgtatcc acgacattca
     4981 acctgttatg cattaaacgt attgcggggc aattttgtgc gtcccgcaac atctttcccc
     5041 gtcattttgt tactccgctt acatcaccca gattgatagt aaaagtttgc aacaagggtg
     5101 aaagtcagta caatccctgc ccgaatgtgt gtaaacgtga acgcaatcga ttacgtaaat
     5161 gatagaactg tgaaacgaaa catatttttg tgagcaatga tttttataat aggctcctct
     5221 gtatacgaaa tatttagaaa cgcaatttgc gcctttttca ctcccgcaag ggattttcaa
     5281 acagtggcat acatatgaaa aaaacattac tggcagccgg tgcggtactg gcgctctctt
     5341 cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca gtatctttcc gactggtggc
     5401 accagagcgt taacgttgtc ggaagctatc acacccgttt tggaccgcag atccgcaacg
     5461 atacctacct tgagtacgaa gcattcgcta aaaaagactg gttcgacttc tatggttatg
     5521 cggatgcgcc ggtattcttc ggcggtaact ccgatgccaa aggtatctgg aaccacggct
     5581 ctccgctgtt tatggaaatc gaaccacgtt tctccatcga caagctgacc aatactgacc
     5641 ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa ctacatttac gacatgggtc
     5701 gtaataaaga tggtcgccag agcacctggt acatgggtct gggtaccgat atcgacactg
     5761 gcctgccgat gagcctgtcc atgaacgtct atgcgaaata ccagtggcag aactatggcg
     5821 cagcgaacga aaacgagtgg gacggttacc gtttcaaaat taaatacttt gtgccgatta
     5881 ccgatctgtg gggcggtcag ctgagctaca tcggcttcac caacttcgac tggggttccg
     5941 atttagggga tgacagcggt aacgcaatca acggtattaa gacccgtact aataactcta
     6001 tcgcttccag ccatattctg gctctgaact acgatcactg gcactactct gtcgtagctc
     6061 gttactggca cgacggtggt cagtggaacg acgatgcaga actgaacttc ggcaacggca
     6121 acttcaacgt tcgctctacc ggctggggtg gttacctggt agtaggttac aacttctgat
     6181 tatgaaaatg ccgggattta ttcccgggat ttattcccgg catttcagat tgttaacaac
     6241 gtgcgctttg ttcatgccgg atgcggcgta aacgctttgt ccggcccaca aaagcacgta
     6301 aatgcaatat attgctgaaa taacgtaggc ctgataagcg tagcgcatca ggcaatttta
     6361 cgttttaatc acttcttctt gttcatcatc gctttcaggt cagcgaatgg gttgtacttc
     6421 gcttcaccga catctttctg cgcatcttcc cctgcgataa cggtcgtacc atactgatcc
     6481 gcttcggtat atttcgaatg ctcatgatcg tggcaataga gacaaagcaa ttcccagtta
     6541 ctgccatctt ccgggttatt ggtatggtcg tgatcaatgt ggtgaacggt aagttcacgc
     6601 aggttggaat aaacaaactc gcgggaacag cgaccgcaga cccacggata gatttttaat
     6661 gctttttcgc gatagccgct ttctaaccgc gcatagtttt ttgggatgat agccatgaaa
     6721 agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa
     6781 tctccataca tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat caacgggaac
     6841 ttgattaagg cagaattgac ggctgatccg ccccttcttt ttccactttc tgctgcaaca
     6901 tgtgttcgcg cttcataccc agtttcagag ccaacgcgga tgccacatag atggaagatg
     6961 cagtaccgat ggaaacacca atcagcatgg tcagcgagaa gccttccagt accgggccac
     7021 cgaagaggta cagcatcagg ataaccatca aggtagtacc ggatgtgatc aaggtacgat
     7081 gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga cgtatcttgc
     7141 ggaagttttc acgaatacgg tccgatacca cgatactgtc gttaagcgag taaccgataa
     7201 ccgacatcaa cgatgccaca atggtcaggt caatctcgat atggaataac gacaaaatac
     7261 ccagcgtaat gataacgtcg tgcgccagcg caataaccac ccctgccgcc agtcgccact
     7321 caaagcggaa acctacgtac acgaggatag acagcagcgc tgccatcaac gccatcgcac
     7381 cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc ttcactgctg
     7441 cattctgatt ggtggattcg ttaatcacct tcagaacctg gctgcccagc acctgaccgc
     7501 cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt
     7561 gcagcattgg ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg tcaatttcag
     7621 ccggtttttc gagcgtaatt tcgataaccg taccaccggt gaaatccagc ccccagttaa
     7681 agccgcgcac gcccataata acgatagcag cgattaacag cagaccagag atgccgaaag
     7741 cccagtagtc ccagcgcata aagtcataga ctttacggcc gtggtttagt tgttcaacag
     7801 tatattcctg tgccacatcg cactcctcag attgacagct tcttgacgcg cttgccgcca
     7861 tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca
     7921 ccgataccgg tagtaatcgc gaacccttta attgccccgg tacccactgc gtacaggatg
     7981 atgactttaa tcagcgtggt gatgttcgca tcgaagatag aactgaatgc gccacgataa
     8041 ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca actcttcttt aatacgttcg
     8101 ttgatcagta cgttcgcatc gaccgctacc gcaagggtta agacgatacc cgcgatacct
     8161 ggcatactca gtgttgcgcc tggcaacagc gacataatac cgacgatcaa gatcaagttg
     8221 gcaatcagag cactggtcgc aatcagacca aacttcttat agaagatgat catgaacaga
     8281 atagaaacca gcagaccggc aaggcaagcc tccagcccct gctcaatgtt ctgcataccc
     8341 agggtcgggc caattgtacg ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca
     8401 cgcagcagca gtgacagctg acgggcttcg ttcgggttgt tgatgccggt gatacggaag
     8461 ctgttaccaa gacgagactg gatgttggcg atgttaatca cctcttcctg tttcaccaga
     8521 accgcacgac catttgcatc tttcttaccg ctgtctttgt actccacaaa cagggttgcc
     8581 atcggtttgc cgatgttgtc cttggtgaag ttagacataa tgttgccacc agcgctatcg
     8641 agcgagatgt taacctgcgg ctggttgtat tcgtcctggc tggaagtgga gtcggtgata
     8701 tggtcaccgg tcagaattac gcgtttgtac agcacaaccg gctgaccttc gcgggtctgt
     8761 ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag cggcctgatc aacgttggtg
     8821 tttaccagac ggaattccag cgttgccgtc gcacccagaa tctctttcgc acgcgcagtg
     8881 tcctgaatac ctggcagttc aacaacgata cggtcagcac cctgacgctg aaccaccggc
     8941 tccgccacgc caagttggtt tacacggtta cgcaggatat tgatgttctg ctgcaccgca
     9001 tattcacgcg cttcactcag acgagcatcg ctcataaccg cacgcagctg gttgctgccc
     9061 tggctactaa tcaccaggtc cggatggcgc ttgctcagat acgcaatggc ttcatcacga
     9121 gctttagcat cgcggaaagt gatgctcagg ccgtagttgt tttctttacg aacggtggta
     9181 tacgggatgc ctttttcgcg caggtcgctg cgcaggctat cgatattttg ttcctggagt
     9241 ttgccaagcg cggtgtccat atccacttcc atcaggaagt gaacgccgcc acgcaggtca
     9301 aggccgagct tcatcggctc agcgtgaata gctgccagcc agcgcggcgt tgccggggca
     9361 aggttaagcg ccacgacgta tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc
     9421 agctgggtgt cagtggagtc gaagcgcgca agaatagcgc cctcttccag tgccacagac
     9481 ttagcagtta ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg
     9541 gcggcgactc cgcgcgcacc agtgatctga acagccggat cctcaccaaa caggttggga
     9601 agcgcataca gcagaccgat gacaatcacc acgatcagca tgacgtactt ccacaaagga
     9661 taacggttta acacggcaat tcccttaggg aaaaatttta attacagcgc cttcatggtg
     9721 cctttcggca ggacggcagc tacgaagtca cgtttaataa ctacttcagt ggtgtcattc
     9781 agcgcgatag caatgtagcc gttttccgct actttggtta cgcgaccgac caggccaccg
     9841 ttcgtcagaa cttcatcacc tttggcaatg gagtccatca gctttttgtg ttctttggtg
     9901 cgcttctgct gtggacgcag gatcatgaaa tagaaaatca gaccgaatac caccagcatc
     9961 aaaatcaaag acatcgggct accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa
    10021 ataaaaaagc tcattaaatt tccctcatta ttaatattaa tcaacgttca aaggtggtac
    10081 ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc tctaatttac cctcttcaat
    10141 agccttgcgt aaacccgcca tcaaacgctg gtagtaacga aggttatgaa tggtattgag
    10201 tcgcgcgcct aatatttcgt tgcaacggtc aagatgatgc aagtaagcgc gtgaataatt
    10261 gcgacaggtg tagcaatcac actcaggatc gagtgggcca gtatcgctct tatacttcgc
    10321 attgcggatt ttcaccacgc catcggtcac gaacaaatga ccatttcggg cgttgcgggt
    10381 tggcattacg cagtcaaaca tatcgatacc acgacgtacg ccttcaacca ggtcttctgg
    10441 tttaccaacg cccatcaggt aacgcggttt gtctgccgga atttgcgggc atacatgctc
    10501 cagaatgcgg tgcatatctg ctttcggctc acccacagcc agaccgccga cagcgtagcc
    10561 atcaaaaccg atatctacca gacctttaac agaaatatca cgtaaatctt cgtaaacgct
    10621 gccctgaatg ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac gctcacggct
    10681 acgcttcgcc caacgcagag acatctccat ggagcgtttt gcgtaatccc agtcagcagg
    10741 atacggcgta cactcatcaa agatcatgac gatatccgaa ccaagatcgt actgaatctc
    10801 catcgacttt tccgggtcga ggaaaatcgg gtcgccgttg atcgggttac ggaagtgcac
    10861 gccctgttcg gtgattttac gaatatcgcc aaggctgaag acctggaagc cgccggagtc
    10921 ggtgaggatc ggtcccttcc actgcataaa atcgtgcaga tcgccgtgca gtttcatgat
    10981 ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg aggataattt gcgcgccagt
    11041 ggcttcaact tcttctggcg tcatcccttt tacggtgccg taggtgccaa caggcataaa
    11101 acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc
    11161 ggtggtgtcc agttcaaatt tcattttttc tccactacgt cagaaaaaca gtccaacgtt
    11221 taaaccagcg ccgcggaatt actccccgac gcgctcatta attgcctgcg gattgtacgt
    11281 gataaacatc gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg ccgctttata
    11341 ggcattcatg gtgtgttgat aaccggcaaa ggccgaaacc agcataatca gcgtcgactc
    11401 tggcaagtgg aagtttgtca ccagcgcatc gaccactttg tactggaagc ccggatagat
    11461 aaagatttgg gtatcgtcaa agaacggttc gatgagatcg tttttcgccg cctgagccgc
    11521 gctttccagc gaacgtactg aggtagtgcc aaccgcaatc acccggttac cgcgcgcttt
    11581 cgccgccagt accgcgtcta ccacatcctg cggtacttca gcgtattccg agtgcatgat
    11641 gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcagggt
    11701 gacgaacgcc atctccacgc ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg
    11761 cagaccagcg gtcggggctg caaccgcgcc cggtttttcg ctataaacgg tttgataaag
    11821 ttcgcggtca gcgtcttcgt ccggacggtc gatatacggc ggcagcggca tatggccgat
    11881 gctgttgaga atatccagta ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc
    11941 gtggcgcgcg gtcattgttg cgttgatact ttcgtcatcg cccagcaaca gttctgcgcc
    12001 aggttttggc gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg
    12061 ctcaaccagc acttcaatct tgccgccgct ggctttacgc ccaaacaggc gcgccgggat
    12121 cacgcgggta ttattaaaaa ccagaagatc gccggggttg agcttatcga gtaaatcggt
    12181 gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac gacagctact
    12241 gcgttcaggc atcggatagt gggcaatcag ggattcgggc aattcaaagg agaaatcggt
    12301 aacgcgcatg acactgactc agactaaaat aagaggcggg tagtctagtg ccggggcgct
    12361 ctccctgcaa cctttacccc cctttggata gaatataatc gatgaatttt ttagctcacc
    12421 tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa tttgctggct gatttcgtac
    12481 gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc tggcattcat atgcatcgac
    12541 gtatcgacgt attgactgac aatctgccgg aagtccgcga agcacgggag tggtttcgta
    12601 gtgaaactcg ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc
    12661 gtcactggtc gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg
    12721 aacaagtgat aacgattttg ccggactcac cgccacgctt tatcaacctg aacaactact
    12781 tatggtcaga gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa
    12841 atggcatggc aagccgtcgc ccacgtctgg atgccctgcg tgactcctgg tacgatttaa
    12901 acgcccatta tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc
    12961 aggcgtcacg caaggcgctc tgaaacgtca aactccggcg tcactacttt ttagcattcg
    13021 catccagcgg ggcggcataa caacggtaag aaaaacattc gctggaatga ttggcgatat
    13081 ctttaaagtt gctttcagca accccctcga acatgagtac cgttctggac aactctccgt
    13141 tgtcatttat atgtggaaaa tcaaattgtg tactgaatcg ccatacatct gtcttttctg
    13201 gctcgctgtt cacagagata cgtcgatgga agactcctcc tgaaaacgtg cgccagaaca
    13261 attgttgatc aaaatcttgt aatatcgcac acttggatgc tatttgttgt agtaaacctg
    13321 cataccgtgg actgccatca cgagcaacat tatacaaaca ctgactaaaa gcatcgaaca
    13381 aacaatagat gtgatcacta gtgaaaaaag gtcttaattt tttttgttct tcgtcgctgt
    13441 aatccaggag catctcattg atttcttcct gtgagaattt taagacaaaa ttatcttcag
    13501 gatttgtttc tttgttgttt ttttcaatat tcttctcctc aatgctattc gcattttgtt
    13561 tcgcaattat ttccgaattt tttttcgcag ttttttccgc agttttttcc gcagtttttt
    13621 tcgcaatttc ttctgcaatt ttttcccgag tttttgattt tagactaaca ccatcgatgt
    13681 caaccggtgt tgttttcaga tctctctgcg cgtctttcaa atttgtctta tagagatcgg
    13741 gcactttatc ggcagatagg tatccggatg aagaaatctg gtgtgtacac atggggggct
    13801 gttcacaaag catcgaggtt atatcgtctt taaactcttt tacattaaac ttctcggcat
    13861 taaccttccc tgttataaac cccgctttgg caattttctt ctctaattgt tttgtacgtt
//
LOCUS       DAZGBC010000129        13811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGBC010000129 DAZGBC010000000
VERSION     DAZGBC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.130"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(519..758)
                     /locus_tag="ACHK1J_003692"
                     /pseudo
     CDS             complement(519..758)
                     /locus_tag="ACHK1J_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(1298..1660)
                     /locus_tag="ACHK1J_003693"
     CDS             complement(1298..1660)
                     /locus_tag="ACHK1J_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912912.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(1651..2187)
                     /gene="yfdR"
                     /locus_tag="ACHK1J_003694"
     CDS             complement(1651..2187)
                     /gene="yfdR"
                     /locus_tag="ACHK1J_003694"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7912913.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YNVAQHAVLCSKLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            complement(2315..3139)
                     /gene="yfdQ"
                     /locus_tag="ACHK1J_003695"
     CDS             complement(2315..3139)
                     /gene="yfdQ"
                     /locus_tag="ACHK1J_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO7912914.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAQFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(3205..3567)
                     /gene="yfdP"
                     /locus_tag="ACHK1J_003696"
     CDS             complement(3205..3567)
                     /gene="yfdP"
                     /locus_tag="ACHK1J_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO7912915.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(4236..4910)
                     /gene="ymfK"
                     /locus_tag="ACHK1J_003697"
     CDS             complement(4236..4910)
                     /gene="ymfK"
                     /locus_tag="ACHK1J_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7912916.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            5001..5201
                     /gene="ymfT"
                     /locus_tag="ACHK1J_003698"
     CDS             5001..5201
                     /gene="ymfT"
                     /locus_tag="ACHK1J_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO7912917.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            5245..5796
                     /gene="ymfL"
                     /locus_tag="ACHK1J_003699"
     CDS             5245..5796
                     /gene="ymfL"
                     /locus_tag="ACHK1J_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO7912918.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            5942..6121
                     /locus_tag="ACHK1J_003700"
     CDS             5942..6121
                     /locus_tag="ACHK1J_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7912919.1"
                     /translation="MRELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            6084..6629
                     /locus_tag="ACHK1J_003701"
     CDS             6084..6629
                     /locus_tag="ACHK1J_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO7912920.1"
                     /translation="MILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIGVLELIKAIYDA
                     PASFLSSAFAHIKVMVGWAGASKDAPVSLITGYANPVQFTTSEIGVSGGGSISPIKEA
                     AIMATVPALTRLNDEDLHKLSYVTTALRALRKVTLSDPQAHQVLVETLINLQAERIRL
                     ADKANFHIHRLLNISGGHRHA"
     gene            6622..6858
                     /locus_tag="ACHK1J_003702"
     CDS             6622..6858
                     /locus_tag="ACHK1J_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912921.1"
                     /translation="MLNPLILNIYRLFQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLITFRSGKNMKHLGGAA"
     gene            6855..7673
                     /locus_tag="ACHK1J_003703"
     CDS             6855..7673
                     /locus_tag="ACHK1J_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7912922.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKSSYDNVDYSQIPAGFRW"
     gene            7670..8164
                     /gene="yfdN"
                     /locus_tag="ACHK1J_003704"
     CDS             7670..8164
                     /gene="yfdN"
                     /locus_tag="ACHK1J_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7912923.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMEAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            8164..8817
                     /locus_tag="ACHK1J_003705"
     CDS             8164..8817
                     /locus_tag="ACHK1J_003705"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7912924.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGTAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            8814..9140
                     /locus_tag="ACHK1J_003706"
     CDS             8814..9140
                     /locus_tag="ACHK1J_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO7912925.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKA"
     gene            9137..9526
                     /locus_tag="ACHK1J_003707"
     CDS             9137..9526
                     /locus_tag="ACHK1J_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7912926.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            9546..10355
                     /locus_tag="ACHK1J_003708"
     CDS             9546..10355
                     /locus_tag="ACHK1J_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO7912927.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVQELTDTQNLGVGPVNTVRGGPKQGSYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVVSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLRQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            10363..11352
                     /locus_tag="ACHK1J_003709"
     CDS             10363..11352
                     /locus_tag="ACHK1J_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7912928.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLVLKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVTFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            11366..12118
                     /gene="ydfT"
                     /locus_tag="ACHK1J_003710"
     CDS             11366..12118
                     /gene="ydfT"
                     /locus_tag="ACHK1J_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7912929.1"
                     /translation="MNLEALPKYYSPKSPKLSDAAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKAGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEPCAGTGFHNVLREVVKYSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARCHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTR"
     gene            complement(12390..12479)
                     /locus_tag="ACHK1J_003711"
     CDS             complement(12390..12479)
                     /locus_tag="ACHK1J_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912930.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLTVIALLIM"
     gene            complement(12534..12746)
                     /gene="cspF"
                     /locus_tag="ACHK1J_003712"
     CDS             complement(12534..12746)
                     /gene="cspF"
                     /locus_tag="ACHK1J_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO7912931.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            13047..13262
                     /gene="cspB"
                     /locus_tag="ACHK1J_003713"
     CDS             13047..13262
                     /gene="cspB"
                     /locus_tag="ACHK1J_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO7912932.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNFRT
                     LFEGQKVTFSVESGAKGPAAANVIITD"
     gene            complement(13626..13796)
                     /gene="ynfU"
                     /locus_tag="ACHK1J_003714"
     CDS             complement(13626..13796)
                     /gene="ynfU"
                     /locus_tag="ACHK1J_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO7912933.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT         3530 a         3124 c         3516 g         3641 t
ORIGIN      
        1 gagtattggt tgagccgagc aggaaaagaa acagagattt aatttgttca gtatataaaa
       61 gctaattttt aaggagtaat tttcagatga ttaaagctga ttacattgcg ataggtactc
      121 tacgactaac ttttcaacaa gagtaagggg gtaatcaatt ttatttgtat caatggtatg
      181 acatttcttg gcgataatgt tttgatctta tcgattcatg tcatatggtc attggtaacg
      241 tccgctcttg gtaaagagtg gacgctcaga ttaggtttgg catctaagca tagatgtgtc
      301 agctcacatc taagctaata catattactc aatcactccc gcaaatctgt aaatcttgtg
      361 tgatgcccat ttatttgggc aggatttaat atcaggatct ggaaggtcag gcctgtattt
      421 ctgaccagtt ctcctgttta cgctgttcca gcgaagaact gtcgatactg aaacaccaca
      481 gaaatcggcg acttgtttag ttgtcattaa gttgttcatt acttcacctc ctgcggcggc
      541 tccggtagtg gcatccagtg ggttactttc gatgcctgtt cttcctcatt gtcagtaact
      601 gcccaccatt tgtttctcga ccaatcgtaa tacccttcga aggtatcgca ctcagtccag
      661 ccgtaagact tcccccaaca ccaaacatac tgtttatcat tcggcattcg ctcactacag
      721 cttatccaac cacccggact taccggagag ttgccagaca gcgcgttctg cagccgttcc
      781 agcttaacgt attcctgaac cctgtttccg tcgcacacct gaagccattg cgcagccttt
      841 tgcgcatcag tgtgaaaggc acaagtgcga ccgtcatcaa attgcatttc gtagaggtca
      901 gcaacttgtt caaactgcgt gtgtggtgac ttgtaagttt ggtttacagg ttcggcacaa
      961 tgaagcatgg cggcgcggca ggcgttccag ccttcatcaa aaccgactat gccattattt
     1021 aaagacggac gagcatctgg cactaccggc actggcttgg ctatatatag cggctgaaca
     1081 taccagccct ttgataacca actgtcagca atgtttttac tcctcgttat tgccggaata
     1141 cctaagccat tttctgaatg cagccatgcc accggctcct cttctagcga tgccagagca
     1201 atttcataag cacggcgctc aatatcgtct cgaacctcta ggctgctgat tcgttctttg
     1261 atttctttaa tcagttcttt attggtaaat gtggtcatta tgctccagcc tccggtgctt
     1321 ttggcattac tgcccagtga gtgatattga cgttttcaag gtccccgacc tgaaatgtcc
     1381 actgccattc tccggtttct ttttgtcccc aggtgtacca gagagaacgc cagccaatca
     1441 gccagccttc tccattagca tcaaataaca gaacactttc atttgctggt ggcagttcag
     1501 ctgacactgg tattattttg ttttccagtg ccgcacattt agcttcaagc gcgtcgaatt
     1561 tacgtaccag gtactcagca tttgtttcgt tcactttcag atctcgcggt acacatttcc
     1621 cgcgaagaaa cccttccatt tcgaaaacat tcatgcgcat ttgcgtaact ccgataaatc
     1681 gttaaaacgt tccataaaca tcccgtaggc atggcccggt gccagtggaa tcacgttgaa
     1741 catctctgtt gccgggatgc cttccagtac aggccagaaa gagccatcat caagcccgag
     1801 atcgcggcgt tcggttgcca gcatgatgag atcggcatat ttcacgggtg tactcataac
     1861 cgggggtaac ccgtatttct cacggattac ggcgtctatt ttttcttcca tccgtttata
     1921 gtcaggaaga aggcgtttca gtggtgcggg aatgtcctgg caatacgctt ctgttgcatc
     1981 atgcattaac gcttcaaaag caaattcctg cggcaccagt ttgctgcaaa gcaccgcatg
     2041 ttgggcgacg ttgtagaagt gcgaaagatg accggcaaag cgacagatat ttgaaaggga
     2101 aaccgcgata tcgttaatat cgatgtcgtc tttatttatc ctgtcataat aaaaatgctt
     2161 cccagaaaaa gttttgataa atgacatttt gttctccacg tatatgcgct gcaccgcgct
     2221 gaattctggt aaaaagaatc cttcaccatc cggcgattat tgagtaaatt acgtttccat
     2281 aaatgccccc gcaggggcat ttgcagtaat gaaatcaggc ggtgaaagta ccaataaagg
     2341 tttctacttt gctgtccttg aatttctcaa caagcagatc acgaaattcg ttagccattt
     2401 cctcctgcac cgcctccagc tgaataatgc gcagaaccag tacaggacga tcgccagtga
     2461 taatgctgag gcgtaattta aacggacgtt ctttcagacc ttcaaacgga acgcatttaa
     2521 attcaaatgc cactggcata atgtctttgg tcttcgcttc gacagactcc atcagggagc
     2581 gtttgccgct gaagtcatta tcttcaaaat cagcggtctg gtttgcttca atcgtgattt
     2641 tacggaccgc cgcagccgct tttgttgcct gaatggcgtc accattagca tcaaagccca
     2701 caaggtagtc ggcccagtct tcgatccatt ctgccagtga cttctgggag ttacgctcgc
     2761 cgttaacaga caacagagca gagaactgtg ctgtcttttt cagtttgaga gtggcagtgt
     2821 tatctgcgtg acctggctca tcaatggtac ccaggttaag cacactgacg gcacgcatat
     2881 tatcggcatc gataaagcag cgggtgcctt catctgcaag atctttagaa taacgggtaa
     2941 agtcatcgat gctggcagtg gaaagcgcac cacggaaacg gaagcgattt aaattaaatt
     3001 tttccagatc atgaatgcgg aaattctcag gcaatgccac agcatcggca ccaatcttac
     3061 tgataatttc attaacaccc tgagcagaaa taagggcatg gatttgatta attgcggttg
     3121 cgtctaagtt ctgagacata ataagtcctc actatataaa gatattcagt gatgagataa
     3181 ataatcagtt aattaagaac gatattaatg acctgctgcg cgtagttttc cgtcaggttc
     3241 accggcaaga gtcagtaatt gtccctggtc ttcctgcaga atagtcaggc gaccaccgcg
     3301 attgacatac atcggcgttt cggtggtgtc ttcttcggaa attttcccgc ggttagtcgg
     3361 gcgaacatat gagagtttgt gtttgatttt cacacggttc tcatcaaatg gttcgatttc
     3421 caggttgagt gagaccttac ctttggtttt cgtgttcatc acaccggaag cgacttcact
     3481 gagaactgcg ccgattttgg tttcaaatac gccgccgtcc agctccccga taaatgcctg
     3541 cacatcagta ctgcgttcgc tagccatttt gctgctcctc atcatatcga ccctgcaagg
     3601 ccggttggtt tctccacaaa acagagaaga acacctgcgg tggcagccgc ccggatggat
     3661 tgggttatga gcccgtcgtc cggtgatgct cttctctgtt ttgtaaaaag agcggtacca
     3721 gccggaagca agtgtacaaa ctggtaccgc caaagcagtg gctgttgtgg tgggggtgtc
     3781 actcaggcgt atggtcaacc tgacaatccg gtgtcctcaa cggggaaaga gtaacccagc
     3841 catacttacc gccgcgccat ttcgcggatg accacaacgc tgagagcact tagccagtta
     3901 cggcaccaca ctttgtcgcg gctccataaa tgccctcatc gttgcaccct ggtctcttcc
     3961 caggcgtcaa accggatcgc cacgctggtt aggcgtctta tcagcatcat cattgacttg
     4021 cacattccgg ctacctggtt tgtttgcccg agcaaggagt ggattgtccc ctttaacgtc
     4081 cccagaccgc taacgacgca tgtgccatac gccgtgttac aaccaaattt tgtttgaatc
     4141 ttgcctgcct catgtttctt ttggatacat tatgtatcta atgggtacat tgtcaagtat
     4201 aaaaaaacct gccgaagcag gttcataaat attgattagg cctttattgt gtatcttctt
     4261 ggttttcccg agaaaatcac tgtaccaatt atagagcaat taccgttaat cttaatgtaa
     4321 ggctcaggcc agtttgggtt taatgctttg agataacgct gtgttccatc ttctatcaat
     4381 cgcttgaagg tggtttcgcc tgtatcgtgc atcaatgcaa taacgtcgtc accgtggcag
     4441 gcagggactt cgggatcaac aaaaatcatg tctccagggc ggtactcatc aatcattgaa
     4501 tcaccaatca cccgcaagat ataagtcatt tcgccacagg gtacagggca gggatacgtt
     4561 tctgctgtgc tcaaatcaac ctcagaatag ccaacttctt tccatgctcc ggcctgtacc
     4621 catgatatga cagggactaa cgttatttgt ttgttagtga ttgaaacatc aggttttttt
     4681 gtgatgttcg ttgtctggtg ttcttgatca agccatccga caggcaggtc gaaacatttt
     4741 tcgatgtgcc gtgccatgct gtcaccgata tttttagtag cgccatcccc cataaacctg
     4801 ctggtctggg ttggctcgcg atcaatcatg gtggcaaagg atgaatttcc gccaacacca
     4861 tctctcagtt ttctggcgtt agaccgccgg atgtcatgga ttgttttcat aacgaaatta
     4921 aaacctttgt accgataagg tacaagtatc ttgaaggttc atttcaatca tgtaatatgt
     4981 atatcggagg tacatattgt atgaaagcgt attgggactc tttaaccaaa gaacagcagg
     5041 gcgagttggc cggaaaagtt ggctcaacac ctggctactt acggctggtt ttcaatggtt
     5101 ataaaaaagc cagttttgtg ctggcgaaaa aacttgagca atgcacgtca ggtgcaatta
     5161 cgaaatctga cttaagaccg gatatctatc cgaaagatta acagaacacc ttcaattttt
     5221 aaccacagaa cgatgaggct aaccgtgggt aagtatcact ggaaagtaga aaaacagcct
     5281 gagtggtacg tgaaagctgt cagaaaaact atcgcagcgt tgccgggtgg ttacgctgaa
     5341 gcagctgact ggctggatgt aacagagaac gcattattta accgccttcg tgccgatggc
     5401 gatcagattt tcccgctggg atgggcaatg gttttacagc gcgcggctgg cactcactac
     5461 attgcggatg ctgtcgcaca gtctgctggt ggggtgttcg tatcgcttcc tgaaattgag
     5521 gaagtagaga acgccgatat aaaccagcgc ctgctggaag tcatcgaaca gatcgggaat
     5581 tactcaaagc agattcgttc ggcaatcgaa gatggggtcg tggagccaca cgagcagaca
     5641 gcaattaatg atgagttgta tctgtcaatt tcgaagctcc aagagcatgc agcactggtc
     5701 tacaaaatct tttgcgctcc agaaaagagt gacgcccgcg agtgtgcagc tccgggcgtc
     5761 gtggcgtttt gtgtctgtgg agaaactaac gcatgaacag tttaacggca aataaccgtt
     5821 tgtcgcaaca gctggtggtc agtgtcgctg aacacctgtt gttacggcat gaatgcagat
     5881 taccaaatca cctggctgta agtaaccaca gagaacttta cctgactgtg gggggcgagt
     5941 tgtgcgggaa cttaaccgct ggtttcgtga cggaagaggg ctttatgtcc atgttattcg
     6001 ttgggaacca gaaacacagc gcgttatcta tcttcgcaaa gactacccgc atgagtgctt
     6061 tagtcctttg tggaaattca ggcgtgattt tgttgagtgt gaaggaccac cagcacattg
     6121 attctgccat tccgggacgt tacactgttc aggcacctta taaagcgggt gccgggattg
     6181 gcgtcctgga attgatcaag gcgatatatg acgcgccagc gtctttttta tcgtccgcat
     6241 ttgctcacat caaagttatg gtgggctggg cgggggcatc gaaagatgcg ccggtttcct
     6301 tgatcaccgg ttacgccaac cccgttcagt tcaccaccag cgaaattggc gtttccggtg
     6361 gtggaagtat ttcaccgatc aaggaggctg ccatcatggc tactgtccca gccctcactc
     6421 gtctgaatga tgaagactta cataaactca gttatgtaac aactgcacta cgtgctctgc
     6481 gcaaggtaac tctttcggat ccgcaggcgc atcaggttct ggtagaaacc cttattaact
     6541 tgcaggctga acgtattcgt ctggcggata aggctaattt tcatattcac cgtctcctga
     6601 atatcagcgg agggcatcgt catgcttaat ccgttgatcc tcaacattta ccgtttattt
     6661 cagcgtaaaa aaacatcaat tcctacagtt gggcagtggt acaccacgcc tgcagggcat
     6721 gttctacgtg ttagcctggt tgaccgtgaa tgtcagaagg tgatttgtga accgctgggc
     6781 cgtaattacc gcgtcagtat gccgcttata acctttcgct ccggaaaaaa catgaagcat
     6841 ctcggaggtg cagcatgagt atggagctga tggttaaagc gatgaaaatt cgagtgggta
     6901 atccattgcg aaaactggtt ctgatcaagc tggctgataa tgccagcgat cagggtgagt
     6961 gctggcccag ctaccagcat attgctgacc agtgcgagat tagcaaacgt tctgtgatga
     7021 atcatattgc ggccctttgt gagtccgggc tggtaaaaaa agtcacccgg aaaggtgaaa
     7081 aaggtaactc aagtaatatc tatctccttc atctggatgg tgcaggagat tcactagggg
     7141 gtagtgcaaa taattcacta tctggtgcag caaattcacc aggtagtgca ggagttgcac
     7201 cagggggtag tgcaggagat tcacccagaa ccagtcactc ttttgaacca gtcaaagaac
     7261 cagtcaatga accaatagct gttggtgcat cagttgatga gtccgtgcga gttcgttcaa
     7321 accgaccgga atattctccg gagtttgagc aggcatggct ggcatatccc aaacgagctg
     7381 gtggcaattc aaaatctgca gccttcaaag cctggaaagc ccgtttgaat gagggggtaa
     7441 accccgaaac catgctggaa ggtgtgaaac gctatgcggg ctgggtatct gcgatgggta
     7501 acagcggcac acaatttgtg aaacaggctg tcacgttctt tggtccggat cgtcatttcg
     7561 aagaatcctg ggaagttcct gcggtatctg cagccagacg tgaggacccg tacttcaaat
     7621 ccagttacga caacgtggac tacagccaga tcccggcagg attcaggtgg tgatcatgag
     7681 tcttttgaat gacgttcaga aattcattga agcccatccg gggtgtactt ccggagacat
     7741 tgcggatgct tttgcaggtt actcacggca gcgcgtactg cagtcagcaa gcaagttacg
     7801 tcagagtggg cgtgtggctc accgttgtga aggggataca cgcagacatt tcccgcgcct
     7861 gactgagaga gcgcaggagc cggaaccaca accagttcgt gaaaccagac ctgtgcgcaa
     7921 tttctatgtc ggcactaacg atccccgggt gattttgtgc ctgacccgcc aggctgaaga
     7981 actggagtca agaggcttat accgtcgtgc tgcaaccgtg tggatggagg cattccgtga
     8041 aagccactcc cagccagaac gaaacaattt tctggcgcgt cgtgaacggt gtttacggaa
     8101 aagcagcaag cgggctgcat caggtgaaga gtggtatctg tcagggaatt acgtgggggc
     8161 ttaatgagta ataaatattg ccaggcgctg gtggaactgc ggaacaaacc agcccatgaa
     8221 ctgaaggaag tgggcgatca gtggcgcacg ccggacaaca ttttctgggg aattaacacc
     8281 ctgtttggcc cgtttgttct ggatctgttc accgacggtg ataacgccaa atgtgccgcg
     8341 tattacacgg cggaagacaa cgcgctggcg catgactggt cagaacgtct tgcggagctt
     8401 aaaggtgctg cctttggtaa tcccccatac agccgcgcca gtcagcatga ggggcaatac
     8461 atcaccggca tgcgttacat catgaaacat gccagtgcca tgcgtgataa aggcgggcgc
     8521 tatgttttcc tgatcaaagc tgccaccagc gaagtgtggt ggccggaaga tgcagaccat
     8581 attgctttta ttcgcgggcg tattggtttt gaactgcctg cctggtttat cccgaaagac
     8641 gagaagcagg tgccgacagg cgctttcttc gctggtgcta ttgctgtttt cgataagacc
     8701 tggaagggaa cggcaatcag ctacatcggg cgcgatgaac ttgaggcatg tggtgaggcg
     8761 tttctggcgc aggttcgcca gcaggcggaa aaactggtca gggagatggc ggcatgacga
     8821 cattaactca atgccagcag caggtgctgg atatgctgat ttcttaccag aaagaacgtg
     8881 gcttcccgcc aaccaatcag gaggtggcaa ccatgctggg ataccgttca gtgaatgcag
     8941 cggtggagca tcttcgcgca ctggagaaaa aaggcgtcat cacgataaag cgtggcgtgg
     9001 cccgggggat aacgcttcat accgcggtga aggacgacga cagcgaggcg gtcgggatta
     9061 tccgctcact gcttgccggt gaggaaaacg caaggctgcg tgcagcccac tggttacatg
     9121 agaggggcct gaaagcatga agctgatcct gcctttcccg cccagcgtga acacttactg
     9181 gcgacaccct aacaaagggg cgtttgcagg taagagcctg ataagcgcgg cggggcgaaa
     9241 attccagagc gcggcgtgcg cagcaatagt tgagcagtta cgtcgtctgc cgaaaccaac
     9301 gtcggcacct gcttcagtgg agatcgtgtt gtttcctccg gataacagga tccgcgatct
     9361 ggataactat aacaaggcgc tgtttgacgc cctgacccac gcaggtgtgt gggaagacga
     9421 cagccaggtg aaaagaatgc tggtggagtg gggaccggtt atcccggaag ggaaggtcga
     9481 gatcactatc agtaagtacg agaaaacggc gggtgcagcc gcctgattaa gaggagaaac
     9541 gaagtatgaa taatctgatg gtcattgatg gtattgaagt tcgtcgtgat gcttatggac
     9601 gttacagcct gaacgatctg catcgtgccg cagtagcatc tggtgcaaat gccagaacga
     9661 aggagccggg aaagtttctt tccagccaac agactgttga acttgttcag gaattgaccg
     9721 atacccaaaa tttgggtgtc ggtccggtta atacagttcg tggtggtcct aagcagggga
     9781 gttatgtctg caaggaactg gtgtatgcct atgcaatgtg gatcagtccg tcattccatc
     9841 tgaaggtgat ccgtactttc gatatggtag tcagtgcacc ggaaaaatta tccgggcagg
     9901 ctgctgacaa gatgcaggca ggtgtgattc tgctggactt tatgcgtcgg gagttaaacc
     9961 tgtctaactc atctgtgctt ggggcctgtc agaaactcca ggaggctgtt ggcttaccga
    10021 atctggcacc gcgctatgcc attgatgctc ctgctgatgc acacgatggc tcaagtcgcc
    10081 ccacgctgtc gctgagtgca ctgctgaggc agtatggtat ccgcctgacg gctaatcagg
    10141 catatcacca gatggtgaag ctggggatcg ttgaacaacg cgaacgatac agccgtaccg
    10201 cgattaacaa catcaaaaaa ttctggtcgc tgacggcgaa aggctgcatg ttcggcaaga
    10261 acatcaccag tcctgcaaat ccgcgcgaga cgcagccgca tttcttcgaa tcccgattcc
    10321 ctgagctgtt aaagctgctc gataccgttc attgaggtga ccgtgagagc actactgacc
    10381 cctgaaattg ccccgcgtat ggggatcgta ttgttcaggc caggttcaga gctgatgccc
    10441 ctgtttatgc aggggcgtgt cctgctggag cctgagccgg aacgttattc atctttcgcc
    10501 agtggtgccg ttccggcggc atcacaaccg ctggcggatg atcctgccgt tcgggccgtg
    10561 ttccgcaatg aggcagtgat ccgtcgtgct ggtggcgtgg aatgtcttga aagctggtta
    10621 cttcgtgaaa agggctgtca gtggcctcat tccgactggc acagcgagaa catgaccaca
    10681 atgcggcacg ctccgggagc aattcgtctg tgctggcact gcgataacca gctgcgtgac
    10741 cagttcacgg aacggctgga atcaatggca acggataact gtgcccgctg ggtgttgtct
    10801 gtcgtgcgtc gggatctcgg ttttgatgac agtcacgttg tgacaatgcc ggaactgtgc
    10861 tggtggctgg ttcgtaatga tttggcggat gccttaccgg aaagtgcagc ccgtaaggca
    10921 ctgagattac cgaagcctgt tgtgccatct gtcacccggg aaagtgacct tgtgccttcg
    10981 gttcctgcca ccagcatcat ccaggataaa gcgaaaaagg tgctggtgct gaaagtggat
    11041 ccggagtcgc cggagtcttt tatgttacgc cccaaacgtc gccgctgggt taatgaaaag
    11101 tacacgcgct gggttaagac gcagccgtgc gcatgttgtg gaaagccagc tgatgatccc
    11161 caccacctga taggccacgg tcagggtgga atgggtacaa aagcgcatga tctctttgtg
    11221 ttgcctttgt gcagaaagca tcacgacgag ctgcatgcgg ataccgtgac atttgaagag
    11281 aagtatggct cccaactgga gctgatattt cgttttatcg atcgtgcgct ggcaattggc
    11341 gtactggcgt aagtggagaa cgagcatgaa ccttgaagcc ttaccaaaat attactcccc
    11401 aaaatctcca aaattgagtg atgccgcacc ggcgacaggc tcaggtggtt taacgattac
    11461 ggatgtgatg gctgcgcagg ggatggtgca gtcgaaagca ccgcttgggt ttgccttatt
    11521 cctggcaaaa gctggcgttc aggatcctca gtttgcgatt gaaggtctgc tcaattacgc
    11581 gatggcactg gataacccga cattgaacaa attgagtgaa gaaatccggt tacagatcat
    11641 cccttacctt gtgagttttg cctttgctga ttattccagg tctgcggcaa gtaaggctcg
    11701 ctgtgagcct tgtgctggta ctggatttca taatgtattg cgcgaagtgg tgaaatactc
    11761 cagaagcggg gagtctgtta tcaaggaaga gtgggtgaag gaactatgtc agcattgtca
    11821 tggtaaggga gaagtcagca cagcgtgcag agggtgtaag ggtaaaggta ttgtcctgga
    11881 tgaaaaaagg acccggcttc atggcgcgcc tgtttataag atttgtgggc gttgcaatgg
    11941 aaaccggttt agtcgtttac caacgacact ggcgcggtgt catgtccaga agctggtacc
    12001 agacctgact gattatcagt ggtacaaagg atatgcagat gtcattgata aactggttac
    12061 aaagtgctgg caggaagaag catatgctga ggtgcaatta agaaaagtga cgagataaat
    12121 gattttcgcc gaagatggcg acgtaatgct tgcatttttc aaaaaatatg gataaaattc
    12181 tttcaacgat gggctttata tacccgacgt taagaaaaag tagaaaaccc gctgatgagc
    12241 gggtttttgt gctttaaata gggtaataga gatgttgaat ctcattccgg gattcatgtt
    12301 tgtttactta ttatttatcg ggtgactttg cctgatgttt aaaatgtttt cttccagtgc
    12361 tatgtcccta gatacaatga gtctgcatat tacattatta gcagagctat tacggtcaaa
    12421 gtacagcata agcttttaaa gccaatcaac cagtcatcaa gacagacggg gttattcatg
    12481 aaaactctcc atgtttgatt cgatggggcc tgaaattaaa gctttaatat agctcatgaa
    12541 aggtaaacat tggcagctga agggccacgc agaccattta tccggcaaaa ttccacgcgt
    12601 aatccggtgg taatttcttc tgcatcgcgg agattgagcg ctgaaacatg aagctggaca
    12661 tcgatacgac catcggatgg ggtgataaga cccttgccgc ttttgccgtc aaaggttttg
    12721 acaattcctg tcattttacg ggacaaaaaa attccttaat actgataact gggcgcacta
    12781 tacacacgtt cctgaagaaa gctatagttt tttgatgggg ttgaagatgg ctggatgtct
    12841 aaaataaaca ttgcttcata tgttcaacta tgcgttaatg attgcgtcgg tttgaagaac
    12901 agacgatata cgaagtagtt tactaaagca gttctcattt caggtgttat tcacttattc
    12961 cttctttgag tctctccaat taagtacgaa gtcgtttctg ttatacaagc catttatgcc
    13021 gaaaggctca agttaaggaa tgtagaatgt caaataaaat gactggttta gtaaaatggt
    13081 ttaacgctga taaaggtttt ggctttattt ctcctgttga tggtagtaaa gatgtgtttg
    13141 tgcatttttc tgcgattcag aatgataatt ttcgaacctt atttgaaggt caaaaggtta
    13201 ccttctctgt agagagtggt gctaaaggtc ctgcagcagc aaatgtcata attactgatt
    13261 aaaattcata gtttgtctgt atacgataac gaagaaggct gatgcctgag tggagataca
    13321 gacagagtgg tgaatattgg atctctttaa taaatagtaa ggaggtccaa tacatgaaac
    13381 aatggccagc atatttggca ataacttaat caggaaaagt atgctaacca ttgtggtgaa
    13441 gtgcaggttt gctgcatgaa tagttttaca gcagaagcta actgctggca tagcaaaaca
    13501 aagtgcgtaa gtggatgact cccacaaaaa gtaccacaat attaaacccg ctcaggcggg
    13561 ttttttatta tctgctttaa atatgttatt aaaatataaa aaatacttgt tacgaataaa
    13621 atcaatcaag ctacagcttt aagatttgtc tggaatactt tgttgcaatg agggcagatc
    13681 aaaagggcac ctttttgtac tcttgaaaaa ctgtgttctg actcttgggt gcagtttggg
    13741 caggaacatt taacgagata attacggcgt gattttgagt ctttacgttc tgacataggc
    13801 ttttcctgta t
//
LOCUS       DAZGBC010000130        13663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGBC010000130 DAZGBC010000000
VERSION     DAZGBC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.131"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..1246
                     /gene="livK"
                     /locus_tag="ACHK1J_003715"
     CDS             137..1246
                     /gene="livK"
                     /locus_tag="ACHK1J_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO7912934.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1294..2220
                     /gene="livH"
                     /locus_tag="ACHK1J_003716"
     CDS             1294..2220
                     /gene="livH"
                     /locus_tag="ACHK1J_003716"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO7912935.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2217..3494
                     /gene="livM"
                     /locus_tag="ACHK1J_003717"
     CDS             2217..3494
                     /gene="livM"
                     /locus_tag="ACHK1J_003717"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO7912936.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3491..4258
                     /gene="livG"
                     /locus_tag="ACHK1J_003718"
     CDS             3491..4258
                     /gene="livG"
                     /locus_tag="ACHK1J_003718"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO7912937.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4260..4973
                     /gene="livF"
                     /locus_tag="ACHK1J_003719"
     CDS             4260..4973
                     /gene="livF"
                     /locus_tag="ACHK1J_003719"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO7912938.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5092..5838
                     /locus_tag="ACHK1J_003720"
     CDS             5092..5838
                     /locus_tag="ACHK1J_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7912939.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQNKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6198..7514
                     /gene="ugpB"
                     /locus_tag="ACHK1J_003721"
     CDS             6198..7514
                     /gene="ugpB"
                     /locus_tag="ACHK1J_003721"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO7912940.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7612..8499
                     /gene="ugpA"
                     /locus_tag="ACHK1J_003722"
     CDS             7612..8499
                     /gene="ugpA"
                     /locus_tag="ACHK1J_003722"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO7912941.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8496..9341
                     /gene="ugpE"
                     /locus_tag="ACHK1J_003723"
     CDS             8496..9341
                     /gene="ugpE"
                     /locus_tag="ACHK1J_003723"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO7912942.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9343..10413
                     /gene="ugpC"
                     /locus_tag="ACHK1J_003724"
     CDS             9343..10413
                     /gene="ugpC"
                     /locus_tag="ACHK1J_003724"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO7912943.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            10410..11153
                     /gene="ugpQ"
                     /locus_tag="ACHK1J_003725"
     CDS             10410..11153
                     /gene="ugpQ"
                     /locus_tag="ACHK1J_003725"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7912944.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11140..11580)
                     /locus_tag="ACHK1J_003726"
     CDS             complement(11140..11580)
                     /locus_tag="ACHK1J_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO7912945.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            11700..13445
                     /gene="ggt"
                     /locus_tag="ACHK1J_003727"
     CDS             11700..13445
                     /gene="ggt"
                     /locus_tag="ACHK1J_003727"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO7912946.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT         3036 a         3464 c         3910 g         3253 t
ORIGIN      
        1 atcgctatca tcacaaaata ctgcgctaac cccttaatca gacaggcaaa aacagtgcag
       61 tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac aacatcacga
      121 atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga tgattgcact
      181 ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg gcgcgatgtc
      241 cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg cgattaaaga
      301 cattaatgcc aaagggggga ttaaaggcga taagctggtt ggcgtggaat atgacgacgc
      361 ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaacgacg gcattaaata
      421 cgttattggt catctgtgtt cttcttctac tcaacctgca tcagatatct atgaagacga
      481 aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac gcggttatca
      541 acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg caaaatacat
      601 tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac agtatggcga
      661 agggctggcg cgttcggtgc aggacgggct gaaagcggct aacgccaacg tcgtcttctt
      721 cgacggcatt accgccggcg agaaagattt ctccgcgctg atcgcacgcc tgaaaaaaga
      781 aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga tgctgcgcca
      841 ggcccgttcc gttggcctga aaactcagtt tatggggccg gaaggtgtgg gtaacgcatc
      901 attgtcgaat attgccggtg atgctgccga aggcatgttg gtcactatgc caaaacgcta
      961 tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca agaaagatcc
     1021 gtccgggcct tatgtctgga tcacctacgc ggcggtgcaa tctctggcaa ctgcccttga
     1081 gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta acggtgcaaa
     1141 caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat ttgattttgg
     1201 tgtcttccag tggcacgccg atggttcatc cacggcagcc aagtgatcat cccaccgccc
     1261 gtgaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt gtatttcttg
     1321 cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc catcggctac
     1381 accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt ttacatgatc
     1441 ggcagctacg tctcatttat gatcatcgcc gcactgatga tgatgggcat tgataccggc
     1501 tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc ctacggctgg
     1561 agtatcgaac gggtggccta tcgcccggtg cgtaactcca agcgcctgat tgcactcatc
     1621 tcggcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgaccga aggttcgcgc
     1681 gacgtggcgc tgccgagcct gtttaacggt cagtgggtgg tgggccatag cgaaaacttc
     1741 tctgcctcta ttaccaccat gcaggcggtg atctggattg tcaccttcct cgccatgctg
     1801 gcgctgacga ttttcattcg ttattcccgt atggggcgcg cctgccgtgc ctgcgccgaa
     1861 gatctgaaaa tggcgagtct gcttggcatt aacaccgacc gggtgattgc gctgaccttt
     1921 gtgattggcg cggcgatggc ggcggtggcg ggtgtgctgc tcggtcagtt ctacggcgtc
     1981 attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc ggtgctcggt
     2041 gggattggca gcattccggg agcgatgatt ggcgggctga ttctggggat tgcggaggcg
     2101 ctctcttctg cctatctgag tacggaatat aaagatgtgg tctcattcgc cctgctgatt
     2161 ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga gaaagtatga
     2221 aaccgatgca tattgcaatg gcgttgctct ctgccgcgat gttttttgtc ctggcgggcg
     2281 tctttatggg cgtacaactg gagctggatg gcaccaaact ggtggtcgac acggcttcag
     2341 acatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc cagcttttgc
     2401 gaccggcttt ccagaaaggg ttgaaaagcg tttccggacc gaagtttatt ctgcccgcca
     2461 ttgatggctc cacggtgaag caaaagctgt ttctcgtggc gctgctggtg cttgcggtgg
     2521 cgtggccgtt tatggtctca cgcgggacgg tggatatcgc caccctgacc atgatctaca
     2581 ttatcctcgg actcgggctg aacgtggttg ttggtctttc tggtctgctg gtgctggggt
     2641 acggcggctt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac tattacggtt
     2701 tgggcttctg gacctgtctg ccgattgccg ggttaatggc ggcggcggcg ggcttcctgc
     2761 tcggcttccc ggtgcttcgc ttgcgcggcg actatctggc gatcgttacc ctcggtttcg
     2821 gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcggc ccgaacggaa
     2881 tcagtcagat cccgaaaccg acactcttcg gactcgagtt cagccgtacc gctcgtgaag
     2941 gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc gatcgtgtca
     3001 tcttcctcta cctggtggcg ttgctgctgg tggtgctaag cctgtttgtc attaaccgcc
     3061 tgctgcggat gccgctgggg cgtgcgtggg aagcgttgcg tgaagatgaa atcgcctgcc
     3121 gttcgctggg cttaagcccg cgtcgtatca agctgaccgc ctttaccatc agtgccgcgt
     3181 ttgccggttt tgccggaacg ctgtttgctg cgcgccaggg ctttgtcagc ccggaatcct
     3241 tcacctttgc cgaatcagcc tttgtactgg cgatagtggt gctcggcggt atgggctcgc
     3301 aatttgcagt gattctggcg gctgttctgc tggtggtatc gcgcgagttg atgcgtgatt
     3361 tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg atctggcgtc
     3421 cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc gcagcgaaag
     3481 gagagcaggc atgagtcagc cattattatc tgttaacggc ctgatgatgc gcttcggcgg
     3541 cctgctggcg gtgaacaacg tcaatcttga actgtacccg caggagatcg tctcgttaat
     3601 cggccctaac ggtgccggaa aaaccacggt cttcaactgc ctgaccggtt tctacaaacc
     3661 caccggcggc accattttac tgcgcgatca gcatctggaa ggtttgccgg ggcagcaaat
     3721 cgcccgcatg ggcgtggtgc gtaccttcca gcatgtgcgt ctgttccgtg aaatgacggt
     3781 aattgaaaac ctgctggtgg cgcagcatca gcaactgaaa accgggctgt tctcaggcct
     3841 gttgaaaacg ccgtctttcc gtcgcgccca aagtgaagcg ctcgaccgcg ccgccacctg
     3901 gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc tggcctacgg
     3961 tgaccagcgc cgtctggaga tcgcccgctg catggtgacg cagccggaga ttttaatgct
     4021 cgacgaacct gcggcaggtc ttaacccgaa agaaacgaaa gagctggatg agctgattgc
     4081 cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata tgaagctggt
     4141 gatgggaatt tcggaccgaa tttacgtggt caatcagggg acgccgctgg caaacggtac
     4201 gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg aggcataaga
     4261 tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa atccaggcgc
     4321 tgcatgaggt gagcctgcat atcaatcagg gcgagattgt cacgctgatt ggcgcgaacg
     4381 gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgcc accagcgggc
     4441 gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc atgcgcgaag
     4501 cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg gaagagaacc
     4561 tggcgatggg cggctttttt gccgaacgtg atcagtttca ggagcgcata aagtgggtgt
     4621 atgagctgtt tccacgtctt catgagcgtc gtgttcagcg ggcaggcacc atgtccggcg
     4681 gtgaacagca gatgctggcg attggtcgcg cgctgatgag caatccgcgt ttgttgttgc
     4741 ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc gacaccatcg
     4801 agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc aaccaggcgc
     4861 tgaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgttgtgctc tccgataccg
     4921 gcgatgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg taaattatcg
     4981 aatacccggc ggccacgttc tgccgggctc ttcgtccgta tacagacttg tttcaactga
     5041 ccgggaggat taaaaccaga ccacttattt gccacggaaa ggttccctcc catgcattat
     5101 gccgttgttg tcatgcttga attgctttgc tgtattcacg cttaccgtac cggacaagag
     5161 cggtactgga tttttatcat tttctgtttt ccggtgattg gctgcgtggc gtatttcgtc
     5221 atagtgatgc tgcctgaaac gggcgctgac cgtcacgggc atactttgct gatgcgcctg
     5281 cagaacaaac tcaaccctga gcgccacctg cgcaaattaa ccgaagaact ggcgattgcc
     5341 gagacgaatc aaaaccatta tgcgttggct aatgaactgg cgcgtctggg gcgttatcac
     5401 gaagctgttc cccattatca gcaggcgtta agcgggatat tcgcccatga agcggcaatg
     5461 atgctaagcc tggcgcaggc acagtttgct attcaggagt tcgccgcctg tcagcagacg
     5521 ctggaagatg tgatgcgtta taaccctgat tttcagtcgg cagacgggca tttgttattt
     5581 gccagaacgt tagccgcaca agaaaaatac gctgatgcgg aaagcgagtt tgaagtattg
     5641 atttcttatt accccggccc gcaggcgcgt atttactatg cggaaatgtt ggcgaaaatg
     5701 agtcggttac gtgaagccaa cgagcagtat gttgctgtag ttgatacggc taaacgctca
     5761 agacctcatt atagaaaaca ccaccgggaa tggattaaga ccgccaatga acggctgaag
     5821 cagagtgtgg tgcaatgaaa atcttctttg ccgttagacc agtgtcgcgg caatcttttg
     5881 ttaaaattga aaaatgtagc tctcatcggg cagcaaaact ggacgcagaa tttgctgcca
     5941 tttttgctct tcatggtaag gagttctggc gattagctgg ccgttaatcg cctcttttca
     6001 gtgcattctc gggatatccg tgccccatct cccattttcc ctcacccgta ccgtcaccgc
     6061 cttgtcatct ttctgacacc ttactatctt acaaatgtaa caaaaaagtt atttttctgt
     6121 aattcgagca tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg cattcggtac
     6181 aacaagagag ataaacgatg aaaccgttac attatacagc ttcagcactg gcgctcggac
     6241 tggcgttaat ggggaatgca caggcagtga cgaccattcc gttctggcat tctatggaag
     6301 gggaactggg taaagaggtg gattctctgg cccaacgttt taacgccgaa aacccggatt
     6361 acaaaattgt accgacctat aaaggcaact acgaacagaa tttaagcgcg gggattgccg
     6421 catttcgtac cggcaatgct ccggctattt tgcaggttta tgaagttggc accgccacca
     6481 tgatggcgtc gaaagccatt aaaccggtat atgacgtgtt taaagaggcg gggattcaat
     6541 tcgatgagtc gcagtttgtg ccgacggttt caggttacta ttccgacagc aaaaccggcc
     6601 acttactctc ccagccgttc aacagctcga ctcccgttct ctattacaac aaagacgcct
     6661 tcaagaaagc ggggttagac ccggaacaac cgccgaaaac ctggcaggat ctggcggact
     6721 atgccgcaaa actaaaagcc tccggcatga agtgcggcta cgccagcggc tggcagggct
     6781 ggatccaact ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg
     6841 gctttgacgg cacggatgcg gtgctggagt tcaacaagcc ggagcaggtg aaacacatcg
     6901 ccatgctcga agagatgaac aagaagggcg atttcagcta cgtcgggcgt aaggatgaat
     6961 ccaccgagaa gttctataac ggtgattgcg cgatgaccac cgcctcttcc ggttctctcg
     7021 ctaatattcg tgagtacgcc aaatttaact atggcgtagg catgatgcct tacgacgccg
     7081 atgcgaaaga tgcaccgcaa aacgccatta tcggcggggc cagcctgtgg gtgatgcagg
     7141 gcaaagataa agaaacgtat accggcgtgg cgaagttcct cgacttcctc gctaagccag
     7201 aaaatgctgc cgagtggcat cagaaaactg gttatctgcc aatcaccaaa gcggcgtatg
     7261 acttgactcg tgagcagggc ttttatgaga aaaacccagg ggcggatacc gcgacgcgtc
     7321 agatgctgaa taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc
     7381 agatccgcgt gattgtggat gaagagctcg agagcgtgtg gaccggcaag aagacgccac
     7441 agcaggcgct ggataccgcc gttgagcgtg ggaatcagtt gctgcgccgt tttgagaaat
     7501 ctacgaagtc ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg
     7561 tgtaagccgg ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca
     7621 tcccgtccgg tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc
     7681 atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gctgcaaagc
     7741 gtcgatccgt ttggtttctc cagccagttt gtcggcctgg ataacttcgt cgcgctgttt
     7801 catgacagct actatatcga ctccttctgg acgacgataa aattcagcac ctttgtcacc
     7861 gtcagcggtt tgctggtatc gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc
     7921 agtcgtttct atcaaacctt gatgctgctg ccgtatgccg tggctcccgc cgttgccgcc
     7981 gtattgtgga tatttttgtt taaccccggt cgcgggctga tcactcattt tctcgccgag
     8041 ttcggctacg actggaacca cgcgcaaaac agcggtcagg cgatgtttct ggtggtgttt
     8101 gcctcggtgt ggaagcaaat cagctacaac tttctgttct tctatgccgc gctgcaatcc
     8161 attccccgtt cgttgattga agccgcagcc attgatggcg caggcccgat tcgccgcttc
     8221 tttaagattg cgctaccgct tatcgctccg gtgagtttct tcctgctggt agtgaacctg
     8281 gtgtatgcct tcttcgacac cttcccggtg atcgacgccg ccacgtccgg tgggccggtg
     8341 caggccacca cgacgctgat ttataagatc taccgtgaag gctttaccgg actggatctg
     8401 gcttcgtctg ccgcacagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg
     8461 cagttccgct atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga
     8521 cgatattcag ccataccatg ctgatcctcg ggatcgcggt gatcctattc ccgctgtacg
     8581 tggcgtttgt cgcggcgacg ctggataaac aggccgtcta cgccgcgccg atgacgctca
     8641 tccccggcac gcatctgctg gaaaacatcc acaacatctg ggtaaacggg gtaggcacaa
     8701 atagtgcgcc gttctggcgg atgttgctta acagcttcgt gatggcgttc agcattacgc
     8761 tcggcaaaat taccgtctcg atgctctcgg catttgccat tgtctggttt cgttttccgc
     8821 tacgtaacct cttcttctgg atgattttta tcaccctgat gctgccggtt gaagtgcgta
     8881 tcttcccgac ggtggaagtc atcgccaacc tgaaaatgct cgacagctac gccggtttaa
     8941 cgctgccgct gatggcctcg gcgaccgcta ccttcctgtt ccgccagttc tttatgacgc
     9001 tgccggatga gctggtggag gccgcgcgga tcgacggcgc gtcaccaatg cgtttctttt
     9061 gcgacatcgt ttttccgttg tccaaaacta atctggcggc gctgtttgtg atcaccttta
     9121 tttacggctg gaaccagtat ttgtggccgt tgttgattat taccgacgtg gatctcggca
     9181 ccaccgtggc agggatcaaa gggatgatcg ccactggcga aggcaccaca gaatggaact
     9241 cagtgatggc ggcgatgttg ttaaccctga tcccaccagt agtgattgtt ttagtgatgc
     9301 agcgtgcctt cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta
     9361 caggcagtaa ccaaaagctg ggatggcaaa acccaggtga ttaaaccgct gacccttgat
     9421 gtggcggatg gcgaatttat cgtgatggtc gggccgtcag gctgcgggaa atcgacgctg
     9481 ctgcgcatgg ttgccgggct ggagcgggtg acggaaggcg atatctggat caacgaccag
     9541 cgggtgaccg aaatggagcc gaaagatcgc gggattgcga tggtgttcca gaactacgcg
     9601 ctttatccgc atatgagtgt tgaagaaaac atggcgtggg ggctgaaaat tcgcggcatg
     9661 ggcaagcagc aaattgccga gcgcgttaaa gaggcggcgc gcattctgga actggacggt
     9721 ctgcttaagc gccgcccgcg cgagctttcc ggcggtcagc gtcagcgtgt ggcgatgggc
     9781 cgcgcgattg tgcgcgatcc ggcggtgttc ctgtttgatg agccactctc taacctcgat
     9841 gccaagctgc gcgtacagat gcgtcttgaa ctgcaacagc tgcaccgtcg cctgaaaacg
     9901 acatcactct acgttactca cgatcaggtt gaggcgatga ccctcgccca gcgagtaatg
     9961 gtgatgaacg gcggcgttgc cgaacagatt ggcacaccag tagaagtcta cgaaaagccc
    10021 gccagcctgt ttgtggcgag ttttattggc agcccggcga tgaatctgct gacaggccgc
    10081 gtgaataacg aaggcacgca tttcgaactg gacggcggta ttgtgctacc gctaaacggt
    10141 ggctaccgtc agtatgccgg gcgtaaaatg actctcggca ttcgcccgga acatattgcg
    10201 ctaagctcgc aagcagaagg tggcgtaccg ctggtgatgg acacgctgga gatcctcggc
    10261 gcagataacc tggcgcacgg acgatggggc gaacagaagc tggtggtgcg actggcgcat
    10321 caggagcgcc cgacggcagg cagcacgctg tggctgcatc tggcggaaaa tcagctgcat
    10381 ctttttgatg gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg
    10441 cacatcgtgg cggcggtaag ttggccccgg aaaacaccct ggcggcaatc gacgtcgggg
    10501 caaaatacgg tcataagatg atcgaatttg acgcgaagtt gtcgaaagat ggcgaaatct
    10561 tcctgctcca tgacgacaat ctcgaacgta ccagcaacgg ctggggcgtc gcgggtgaac
    10621 tgaactggca ggatttacta cgtgtggatg caggcagttg gtacggcaaa gcgtttaaag
    10681 gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacat gggatgatgg
    10741 cgaatatcga aatcaaaccc accactggca ccgggccatt aacgggcaaa atggtggcgc
    10801 tggcggcacg cgaactgtgg gccggtatga cgccgccgct actgtcatcg tttgagattg
    10861 atgctttaga agccgcacaa caggcagtac cagaactgcc gcgcggtttg ttgctggatg
    10921 agtggcgcga cgactggcga gaactgaccg cgcggctggg ctgcgtctct attcatctca
    10981 attataaatt gcttgataaa gcacgagtga tgcagttgaa agacgccgga ttacggattc
    11041 tggtttatac cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt
    11101 gcatctgtac cgatgcgatt gatgtgattg gtccgaactt tacggcccaa tagttttcaa
    11161 cggtatatca ggctgcggcg tgccgggctg attcagcatg tcgccgtttc tcctctccgg
    11221 caacatatgc tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc
    11281 attggtgttg ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa
    11341 cacccgctga gaattattgt ttatctggtt ttctaaatgc tgttgttgca actgagtttg
    11401 cgttttcagt tgctgattca gcatcccttt ttgctggatt tgctgcgttt gcatctgggt
    11461 ttgcatccgc tgctgactgg ggatctgata ccccggctgg ttagggttgt tcagagtatt
    11521 aatgggctgt gcaaagccga caaacggcag gagtgccgta agaatcagaa gtcgtttcat
    11581 cgcgtatcct cctctgaaga tattctttaa gtttactcct ttcctgcaat catcaggatt
    11641 aattcagagt tatataccag gcttagctgg ggttgcccct taatctctgg agaataacga
    11701 tgataaaacc gacgttttta cgccgggtgg ccattgctgc tctgttcaca ggaagttgtt
    11761 ttagtacggt agccgcgcca cctgcgccgt ctcctgtttc ttacggcgtg gaggaagatg
    11821 tctttcatcc ggtacgcgcg aaacagggga tggtggcatc tgtagatgct actgccactc
    11881 aggtgggggt ggatatcctc aaggagggcg ggaatgccgt tgatgccgcc gtggctgtgg
    11941 gctacgcgct ggcggtaacg catccgcagg cagggaatct gggcggtggt ggttttatgc
    12001 tgatccgctc gaaaaatggc aataccaccg ctatcgattt ccgcgaaatg gcacccgcca
    12061 aagcgacccg cgatatgttc cttgatgacc agggcaaccc ggacagcaaa aaatcactca
    12121 cttcgcatct ggcttccggc acgcccggta cggtcgctgg tttctcgctg gcgttggata
    12181 aatacggcac aatgccgttg aacaaagtgg tgcagccagc gtttaaactg gcacgcgatg
    12241 gttttatcgt taacgacgcg ctggctgacg atctcaaaac ctacgggagc gaagtgctgc
    12301 cgaatcacga aaacagcaag gctatcttct ggaaagaggg cgagccgctg aaaaagggcg
    12361 acaagctggt gcaggcgaac ctggcaaaga gcctggagat gattgccgaa aacggcccgg
    12421 acgaattcta caaaggcacg atagcggaac agattgccca ggagatgcag aaaaacggtg
    12481 gcctgatcac taaagaagat ttagcggcgt ataaagcggt cgaacgcaca ccgataagcg
    12541 gcgattatcg cgggtatcag gtttactcca tgccaccgcc atcctccggc gggatccata
    12601 tcgtacaaat cctcaatatt ctggaaaact tcgatatgaa gaaatacggt tttggcagcg
    12661 ccgacgcgat gcagatcatg gcagaagcgg agaaatatgc ctacgctgac cgctcggaat
    12721 atcttggcga cccggatttt gtcaaagtcc cgtggcaggc gctgaccaat aaagcctatg
    12781 ccaaatctat tgccgagcaa atcgatatca ataaagcgaa accgtccagc gagattcgcc
    12841 ctggcaagct tgcgccttat gagagtaatc aaactaccca ttactcagtg gtggataaag
    12901 acggtaacgc cgtggcggtg acctatacgc tgaacaccac cttcggtacg ggtattgtcg
    12961 cgggcgagag cggtattctg cttaataacc agatggatga tttctccgcc aaaccgggcg
    13021 taccgaacat ttatgggctg gtgggcggtg atgccaacgc cgtcgggccg aacaaacgcc
    13081 cgctgtcatc gatgtcaccc accattgtgg tgaaagacgg taaaacctgg ctggtcaccg
    13141 gtagccctgg cgggagccgg attattacca ccgtgcttca aatggtggtg aatagcatcg
    13201 attatggcat gaacgtcgcc gaagcgacca atgcgccgcg tttccaccat cagtggttgc
    13261 cggacgagct gcgtgtcgaa aaagggttta gcccggatac gctcaagctg ctggaagcaa
    13321 aaggtcagaa agtggcgctg aaagaggcga tgggcagtac acaaagcatt atggttgggc
    13381 cggacggtga gctgtatggt gcatccgacc cgcgctcggt ggatgattta actgcggggt
    13441 attaaggtta gcggccctct tcgtgggaag agggctgttt tcttgaacaa gataaattag
    13501 tgtctggatt gcttttacta ttgctccgta atatataatc ataattatcc tgaatgcttt
    13561 taaggggctt aggttatgct ttcttttcaa gagtatgcag aatagttttt catttaccgt
    13621 gaaaatagga cgtgtgataa ttacttcctg atattcagga aag
//
LOCUS       DAZGBC010000131        13605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGBC010000131 DAZGBC010000000
VERSION     DAZGBC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.132"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..592)
                     /gene="yeeO"
                     /locus_tag="ACHK1J_003728"
     CDS             complement(<1..592)
                     /gene="yeeO"
                     /locus_tag="ACHK1J_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO7912947.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKEAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLIN"
     gene            753..828
                     /locus_tag="ACHK1J_003729"
     tRNA            753..828
                     /locus_tag="ACHK1J_003729"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:786..788,aa:Asn,seq:gtt)
     gene            complement(866..1816)
                     /gene="cbl"
                     /locus_tag="ACHK1J_003730"
     CDS             complement(866..1816)
                     /gene="cbl"
                     /locus_tag="ACHK1J_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO7912948.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1918..2835)
                     /gene="nac"
                     /locus_tag="ACHK1J_003731"
     CDS             complement(1918..2835)
                     /gene="nac"
                     /locus_tag="ACHK1J_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO7912949.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3161..3236
                     /locus_tag="ACHK1J_003732"
     tRNA            3161..3236
                     /locus_tag="ACHK1J_003732"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3194..3196,aa:Asn,seq:gtt)
     gene            complement(3292..4227)
                     /gene="ldtA"
                     /locus_tag="ACHK1J_003733"
     CDS             complement(3292..4227)
                     /gene="ldtA"
                     /locus_tag="ACHK1J_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7912950.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4289..5368)
                     /gene="cobT"
                     /locus_tag="ACHK1J_003734"
     CDS             complement(4289..5368)
                     /gene="cobT"
                     /locus_tag="ACHK1J_003734"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO7912951.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5380..6123)
                     /gene="cobS"
                     /locus_tag="ACHK1J_003735"
     CDS             complement(5380..6123)
                     /gene="cobS"
                     /locus_tag="ACHK1J_003735"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO7912952.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLMALL"
     gene            complement(6120..6665)
                     /gene="cobU"
                     /locus_tag="ACHK1J_003736"
     CDS             complement(6120..6665)
                     /gene="cobU"
                     /locus_tag="ACHK1J_003736"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO7912953.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRKGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQVINTEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            6889..7023
                     /locus_tag="ACHK1J_003737"
     CDS             6889..7023
                     /locus_tag="ACHK1J_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO7912954.1"
                     /translation="MEEISLVKVWLREAGAHNVLVTSAVNNNGVAELLALLHTEEGCR
                     "
     gene            complement(7124..7453)
                     /locus_tag="ACHK1J_003738"
     CDS             complement(7124..7453)
                     /locus_tag="ACHK1J_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO7912955.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(7625..8683)
                     /locus_tag="ACHK1J_003739"
     CDS             complement(7625..8683)
                     /locus_tag="ACHK1J_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7912956.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRAVRK"
     gene            complement(8882..9355)
                     /gene="sbmC"
                     /locus_tag="ACHK1J_003740"
     CDS             complement(8882..9355)
                     /gene="sbmC"
                     /locus_tag="ACHK1J_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO7912957.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(9474..10640)
                     /gene="dacD"
                     /locus_tag="ACHK1J_003741"
     CDS             complement(9474..10640)
                     /gene="dacD"
                     /locus_tag="ACHK1J_003741"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO7912958.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            10849..12276
                     /gene="sbcB"
                     /locus_tag="ACHK1J_003742"
     CDS             10849..12276
                     /gene="sbcB"
                     /locus_tag="ACHK1J_003742"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO7912959.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNLIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGNNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(12319..12546)
                     /gene="tsuB"
                     /locus_tag="ACHK1J_003743"
     CDS             complement(12319..12546)
                     /gene="tsuB"
                     /locus_tag="ACHK1J_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO7912960.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(12560..>13605)
                     /gene="tsuA"
                     /locus_tag="ACHK1J_003744"
     CDS             complement(12560..>13605)
                     /gene="tsuA"
                     /locus_tag="ACHK1J_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO7912961.1"
                     /translation="LSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAI
                     SVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLI
                     GSWIALFTYMVMSAVMRSPHASSLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITL
                     WVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGR
                     MFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQT
                     TLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASWLVYVR
                     PQRKARLATAAAN"
BASE COUNT         3640 a         3624 c         3061 g         3280 t
ORIGIN      
        1 cgttaatcag tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaatta
       61 gagtgatggc agctgctgga taactgagta ccgtcagctc cagataagtc aacgccagtg
      121 ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat aatttgctcg ccaaaatgat
      181 gaataagcgt tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg
      241 ccaccctcgc tcgtcgtcga tcccgcttac cgagactaaa tgccacaacg acagtagtac
      301 caagatcgat agcagcaaaa aaagccataa tgaccatatt gaagctgtcc gccaatccca
      361 cgccggccat cgcttctttt cccagccagc tgaccagaaa agtgctcaga acccccatca
      421 acaggacaca ggcattctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga
      481 acaacacttt gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca acctggcgta
      541 aagcagagga gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaata
      601 atgagggaga atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac
      661 aaactgttga tagaaacggc aaacagttgg ggaatttaaa aatcgggttt gacaaaagat
      721 ttttcgccgt taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc
      781 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa
      841 gcccgctttt atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt
      901 ccatcacctg ccgcttgata tcctcaactg aaagtcctgc attacaaagt tccagaaagc
      961 gccagacata gttacgctga agttgtcctc gcttcagtcc caaccagaca gtattagcat
     1021 caaaaagatg ccgcgtatcc aggcggatta aattcttttc ctcttgttca ccgctggatt
     1081 gttcggcaac tattccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat
     1141 cctgcgcact taatacaata tctgccagca aacctttgcg ggcaaatgcg tcatcaatac
     1201 gtgagcgccc cgtaatcccc tgtcggtaag tgattaacgg ccacttcgct attgactcca
     1261 gcgtcaatgg tgtaatttgc gtcaagggat gatcgagtgg aacaagcaaa ctatggtgcc
     1321 aacgaaacca cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac
     1381 caatatcagc ttcaccatta tgcaacaacg tcgcaatttc ctgtggcgtc ccctggatta
     1441 gctcgagccg aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat
     1501 aacgtgcctg agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca
     1561 ggtctgcaag ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta
     1621 atgctttgcc cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga
     1681 tgccaagttc atcctccagt tcccgaatat gacggctgac gcctgactgt gaggtaaaaa
     1741 gcatattcgc aacctctgtc aggttgtaat cctgacgtgc agcttcgcgg attatcttta
     1801 gttgttggaa attcacggta aactccgggc agttcagata ttccgttatt gttaaagtct
     1861 aacgcccggc ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta
     1921 gctcaccaat tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa
     1981 ctctttcact gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat ttaatgacaa
     2041 agagagactc atggaaggcg tggtaatacg tgacatccac ccatttactg cgccacataa
     2101 cgaacgcgcg gccgattcgg gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt
     2161 aagcgtggca atagactcaa tttcaccaat gacttttgcc gtgagtcgcc gtagggaaaa
     2221 cgcctcatca acacgaagtc taacagcgct gtaatcactg gggagaaaga ggttcatttg
     2281 tgcaatagca ttaacatcaa cgctttgccc cgggcaatct tgagttccta ccaggaaaag
     2341 atcttctttc agcaaagcct gactggatac cccagccaca ggggaatgct cataaatcac
     2401 cgccatatcg agttggtgat ttatcagttt ttcgttaagc actgcaccgc tattttcatg
     2461 aagatagata acgatctccg ggaattcagc gcgaaccgct tgtaataagg gcatggtgat
     2521 agatgacgca gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgcctgacc
     2581 aacgttatgc accgccagtt gggcctgctc acactgacgt aaaatggccc gcgcatgggt
     2641 atagagaatt tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagttg
     2701 ttgatttaac tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg
     2761 caatacttca gcagcctgag tcaggctacc aatatctaca atttttacga agtatttcag
     2821 gcgtctgaag ttcatgttgc ctccggtttt tgagaatcgg cccaagtgcc gccattactt
     2881 acaaccagac tgcaagatgc ttgccagttt tattttggcg ttgatgtaca agctaaccaa
     2941 ctgtcaaata agagattatg atagattcgt cattggctct tttcaccagc agtcgcgttc
     3001 ccctgcccca taaaaggaga gtatgcacca cgatggttca taaccaataa gattgaaagc
     3061 tcaccacttt gttgaaattg acagcaaaca aacaaaaaaa tgcatttcac cctttgacat
     3121 caccatgcac tgccattaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt
     3181 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     3241 caaaaaagcc tgctttctag caggcttttt gctttctaat taccaacgct cttaaaacat
     3301 ctgtcttgaa ccagaactaa tttgcacagg cattcccgat cgacgttgca acgcagcatt
     3361 tgcgcgattc acatcaactt cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg
     3421 caatggcact tttcggtcag actcatattc tgcacgattg cgcgacagcg gctcatgaac
     3481 ttccagccag ttcgagccat ctggttcagt ggtgtatttt actggctggt cgataatttg
     3541 cacacgcgtc ccaacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg
     3601 aatacagccc tgacttaccc ggagcccaat accaaaattg gcattggtac cgtggatggc
     3661 atacaacctg ccaatataaa tcgcgtacag ccccatggga ttatcgggcc ccgcaggaac
     3721 aaatgcgggc aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg gcgttggcgt
     3781 ccaggttgga gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg gggtttctcg
     3841 cccagcctgg ccgataccaa taggaaagac ttccacagta ttactgtctg gtgggtagta
     3901 ataaagacgc atctcagcga cgttaaccac aatcccttta cgaacagtgg cgggcaaaat
     3961 cagttgctgc ggaatggtga gttgcgagcc agacttcggc aagaaaacat cagcgcccgg
     4021 gttcgcttcc agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg
     4081 ctgggtattg tgatcaggaa cagttacagt aaacgactgc cccactaaac ggctaccctc
     4141 tggaggtaat ggataagtta ccgccaggct agtatggctg gcaaaaagca gagcaaatga
     4201 gcaaagaata tttacacgac gcatcatgtc cctttcctat gtcgcgaaag ctatccgtta
     4261 agtatagctt ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg
     4321 gtagaacaat attactggca gcaagttcgc ccatgttgtt gtatatcgca caggcagctt
     4381 cgatgatggg catcgccaga gctgcgccac tcccctcacc taaacgcatc tccatattga
     4441 gataaggctc cagccccaaa tgcgacagcg ctatacgcgc ccctttttct gccgacaggt
     4501 gagaaggaat gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcagcag
     4561 cataagaaag aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca
     4621 cgccagccat tccgaccaaa tcaaatccac ccacttttgc cagaacatca acgccatcct
     4681 gcggatttgg ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt
     4741 tatctgtcgg caggtttgcg ccaatcccaa ccacttcttc aggatcccgg ccagtgattg
     4801 tgctgactat tgctgctgcc ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca
     4861 gcgtgacacc gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt
     4921 cagcctgacg gcgactcatt gccggagctg aagcaatatt gccgctacct cgtgcgacac
     4981 gcatgttgat aagcccgggg ataggctcag cagtatcaat accaacatca attacgtgga
     5041 cgttagcacc cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt
     5101 cagcctgaat ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt
     5161 gatcggcaca cataaccagt accgcttttt tgcccacatg cggtatgcca ttcaaccccg
     5221 gcattcctgc cagttgtatg gcaagcgcct ccagccttcc aaggctacca acaggtttga
     5281 gtaacccgtc aatatgccgt tgtgcacgcg acatagcggc aggatcgata gcagggatcg
     5341 tattcagtaa atcggcaagt gtttgcatgt cacgtccctt tatagcagtg ccatcaggaa
     5401 gaccagttca ccaagctcta tggctgctcc cagcgtatcg ccggtttgcc cgcccagcgt
     5461 acgtttgaga agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac
     5521 accatgcata cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca
     5581 ggtttgtcgc ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata
     5641 acgatgacga tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag
     5701 cgatgcaagg atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag
     5761 tacaacaaaa atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc
     5821 caacatgcga tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc
     5881 caggtggaat cccccggtca tcagcgccag cacaagcacg ctaaacagtg ccgccagtgg
     5941 tacgccacac catgcctgca gcaccatgaa aaccagcccg ctaatcgcgc caagcaataa
     6001 tccaattaaa ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga
     6061 ccagcgacgc ggtacaggca gacgcgtaat aaatgagagc atcgcccaaa ataatttact
     6121 catttgattt ttactccaat acccgaaacc accagccata cttcatttgc cgcagcggcc
     6181 agctgctgat ttacccgccc ggcaatatca cgaaaatgac gtgccagacg actctccggc
     6241 acaatcccca ttcccacttc gttagtcact aatacaacct ttgcggggca acgttggcag
     6301 gcagcaatca acgactgaat ctcagtatta atcacctgtt ccatcgcctg ataatcccat
     6361 tcatcagggt ctttatcgcc gccataatca aacaacagat tagtcaccat tgtggtaacg
     6421 cattccagca acacagcttc atgcgggtta atgtctgcat gaattaattc atcaagatgt
     6481 tgccaacgct ccaccgtgcg ccagtgcgcc gggcggcctt tccgatgatg ctctatccgt
     6541 gcagccatct catcatcaag gatttgggag gtggcgatat acagaacctg tgaagagtcc
     6601 ccaataagcg cctctgcgtg gcgacttttc ccgctccgtg cgccccccgt caccaaaatc
     6661 atcataccga ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc
     6721 ccaccgttgc tgaccgcaaa acatttacac gattaatact gcgcgtaata taattaaaaa
     6781 tccagcattc tcaatccatt acgaaagaag atggcattgt ggaatagcca gacttcctcg
     6841 tgttaacgtc cgatcattgg agtagtcacc aaagcagatt tagccagcat ggaagaaata
     6901 tctttggtga aagtttggtt acgggaagcc ggagcgcaca acgtgttagt aaccagtgcg
     6961 gttaataaca atggagttgc agaactttta gccttgctgc atacagaaga aggctgtcgc
     7021 taatttcgac aggttctcag atgagaatga tgactttgat acacagaaaa gaatcaatga
     7081 gcaatgaggg ttgccgggca accctcattg aataaaacag gaattacttc gcttcgccgt
     7141 ttttcatttc gcccatagct ttcagctttt tggagatatc gcggcgttct ttggagagct
     7201 cggcattttt gatgatgtaa tcgtcaacac gatcttcata gtcacctttc atgctggcga
     7261 tgatgccctg gattgcttca acgctcatcc ccggcttgat gtaatcgctc aggttgtcca
     7321 gcagcaggac gcgcttctgg ttgtcacgga tctttttctc aacgtcctga atttcacgtt
     7381 gcagtttgtt cttacgacgg aacagacgaa caaattccag tacgtcctgg aatgaaggct
     7441 tggtagtttc catttttata cccctgataa tgtgagagtc ggattcgttt aagcaaccgc
     7501 tattcgttag ggccaacatt actcatagct gccgcagatg acaatgcttt tatccctttt
     7561 cactatcata ccctttatcc ttgctgaatc gaagcagcag caagatgatt ctgaagttca
     7621 ggaattattt gcgcacggcc cggcaaatca gattcgatag caactcaagc tgatgtgccg
     7681 tttccatgtt cagccacaca taaccataga ttggtgtttc cacaaccttc aggtcatggt
     7741 ggttattgag caactgacgc agctcttcca cagcatcgtt caatttttcc gtattggcaa
     7801 aaaccggctg cggattacct tcgtacagcg catgaacaag gctcagcagt atttgctgca
     7861 tcatgtgctg ggtatcacga agtttttgcg cgttcaataa cacgaaatgg ctggggcgcg
     7921 tggcccagta cgcattgatt tgcaactcca gcatacaaac cagattgcga ttaattgtct
     7981 ggataccttc atatatcgat tttggaatac gggtttcttt gctggcgggc gcaatcaacc
     8041 cacgcatttt cacggcatcg gtcaggagtt tttgtagatg gctttccaga cgtgggcgtt
     8101 cgagtaagtt cggtgagaat gcagattgat agacccgatt atactcggtc agacttttcg
     8161 ccagttgaat gcgccagtgg atgaacgccc gttgtggcca gataccggta aacaacattg
     8221 ccagtaaaga gccgaggatc acatcgccgc ttcgccataa cgccgtatca atttcacctg
     8281 tcggggaacc aacaacaatt gccagcgtca ccccaatcaa taaaccttga tacggtttct
     8341 tgcccagcgc cagccaaccg caaaggaaca tggccgccgc gcaccagact aacatcagcg
     8401 gtaacgagat taactccagt tgcagagcga taagacctaa aatcgaaccc aacaccgtac
     8461 cgccaatacg ttcgaaggcg cgagggacaa cgttacccca gaacgagatt ggccccataa
     8521 tcaccaccat ggtgaccagc ggccaggtgc tttccgggat agtaaacagg cggataatga
     8581 gaaaagtgag caggaatgcc agcgcgaccc gagtaccatg cacaatgcgg taatggcggt
     8641 atacccggat ttcaaacggg cttaatgact tatcggcacg cacgcagaaa ctccgtaatg
     8701 aagattaaaa gcgctcataa ctcatctgtc ggccagaagg cgcgcctctg aattatccga
     8761 cggacaaacc gggaacagtg taatttcccg gcaggagcga aaaaattgaa aggcgcatct
     8821 tactcttttc gctttcaaaa aaaagagtgg tcatcgcgtt aacacaccgc cctgagatga
     8881 attagtgatg ttttggctgc accgcaacat acatttcgat atcccagtac ccatcttccg
     8941 cgccattgtt caaataaacc tcgaagcagg gctttggtaa catttcataa gcactgtcct
     9001 gcaagaggct attaaagaac tgataccagg gtttagcaaa atcatcaccg actacacgag
     9061 ctaccgccac cgcatactga ccacctgaaa tttctgtcag aatgacgccc tcactgtttt
     9121 cggggagcgt aaagtaaccc ggcaccgtca cgacggtgtc gcagcgtaat ttttcggcgg
     9181 gtgtttcatc tggattgtcg taatagacag caacccattc cttcggcaca atatttttgc
     9241 tatctaccca catcatcaac tgctcaaaac ctttctttac cgtctgttcc cacgggccaa
     9301 cgagatggaa acctgcaacg gtacgtttat cttcctgctt aatctcgtag ttcatgacgc
     9361 ctccattaac actgttttta tatacagtat agttgcaata ttaaaaccac aaggaatgag
     9421 tgttgattat gcgagcagac tcgcactcct gccagtctgc tgcaaaagaa aggtcaggcc
     9481 ttatggtgga aataatcact caggcgggaa aacatgccgc cttccccgac agattccagg
     9541 gtaaccagcg gccagtgcgc cacctgttta tcacggtcgt aaagttcaat ttcccctacc
     9601 cgctgatggg cgctaattgg cgcggtgagc tctttaccat caagggtata tttggctttg
     9661 atatgtggaa tttcggcttt cggtagcacc atccagaact cttgttccgt tcccaggtcg
     9721 atattttctt tatcgccata ccagatgcgt tccgttccga cctttttccc acggtgcaaa
     9781 atttgcaccg tagtaaagtt ttgttgcccc caacgtagta attttcttgc ctcttcctca
     9841 cgaccttttg cactgtcagc ccccattacc acggcaatga gacgacgctg cccatctaca
     9901 gccgaagcaa tgagattaaa cccggcacca gaagtatgac ccgttttcag gccgtcaaca
     9961 ttcatggttt tatcccacaa caacccgtta cggttttgct gggtgatacc gttccaggtg
    10021 agacttttct cactgtacat atgataaaac tcgggctcgc cgtggatgat agcgcgagaa
    10081 agtacagcta aatcataagc cgagctatgc tgacctggcg catccaggcc atgcactgtt
    10141 tcaaaatgcg tatccttgag atgcagcttc tcggcatagt tgttcatcat ttcaacaaac
    10201 tgccgttgcc caccggcaat atagtcagcc agtgcaacac aagcgtcatt tccggaatcc
    10261 acaattaaac cacggcttaa atcacgtacc gatacgcgat cgccctcttt caaaaacatc
    10321 agtgaagaac cgacaaacac cggattatct ttcgcccacg catcgcgccc cacggtgaca
    10381 atatcgtctg gcgtaatgcg atgactatcg atagcgcgat ccacgacata acccgtcatc
    10441 agctttgtca ggctggcggg attgcgctgt tgatgctcat tacccgcggt gaggatctgt
    10501 ccggtggtgt aatccatcag tacccaggac ccggcatgaa tctctggagg ctgaggtgaa
    10561 aaaggaatgt tttccgccgc aaaaccagac gataagttaa aaacgaacaa agaagcagca
    10621 ataataagac ggcgtttcaa cagcaaaccc tcaggagttt caaatagctg ttctttttac
    10681 ggaaatactt atgaactggc tggaataaag tgcaaaaaaa tgtgactacc ctctcatttt
    10741 tatctgacat gatctgttgc cactcgctgc caaattgtgg cgctaaagct gattagcacg
    10801 gtgatatttg atactctggc agacagcaga aataacggat ttaacctaat gatgaatgac
    10861 ggtaagcaac aatctacctt tttgtttcac gattacgaaa cctttggcac gcaccccgcg
    10921 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgata gcgaattcaa tctcatcggc
    10981 gaacccgaag tcttttactg caagcctgcg gatgactatt taccccagcc aggagcagta
    11041 ttaattaccg gtattacccc gcaggaagca cgggcgaaag gagaaaacga agccgcgttt
    11101 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
    11161 gtgcgtttcg acgacgaagt cacacgcaac attttttatc gtaatttcta cgatccttac
    11221 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
    11281 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
    11341 cgccttgagc atttaaccaa agctaatggt attgaacata gcaacgccca cgatgcgatg
    11401 gctgatgtgt acgccactat tgcgatggca aagctggtaa aaacgcgtca gccacgcctg
    11461 tttgattatc tctttaccca tcgcaataaa cacaaactga tggcgttgat tgatgttccg
    11521 cagatgaaac ccctggttca cgtttccgga atgtttgggg catggcgcgg caataccagc
    11581 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggtggatttg
    11641 gcaggagaca tttcgccatt actggaactg gatagcgaca cattgcgcga gcgtttatat
    11701 accgcaaaaa ccgatcttgg caataacgcc gccgttccgg ttaagctggt gcatatcaat
    11761 aaatgtccgg tgctggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
    11821 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgc
    11881 gaaaaagtgg tggcgatatt cgcggaagcc gaaccgttta cgccttcaga taacgtggat
    11941 gcacagcttt ataacggctt tttcagtgac gccgatcgcg cagcaatgaa aattgtgctg
    12001 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acggattgaa
    12061 aagctgttgt tcaattatcg ggcacgcaac ttcccaggga cgctggatta tgccgagcag
    12121 caacgttggc tggagcaccg ccgccaggta ttcacacccg agtttttaca aggctatgca
    12181 gaagagattc agatgctggc gcagcagtat gccgacgata aagagaaagt ggcgctgtta
    12241 aaagcgcttt ggcagtacgc ggaagagatc gtctagtttg aagcctccgc cgctgttacg
    12301 gcggaggaca atgttgggtc aggctttttg aacggtgatg ctccacgcag catcgccaat
    12361 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
    12421 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
    12481 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
    12541 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
    12601 gtcgaacata gacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
    12661 cccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
    12721 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
    12781 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgacccca
    12841 cgaagatccc taacaccaag aaaacgcccc agttaatgta tttcacgtca cctgcgacca
    12901 gaaattgcag aatattggcc gttggggaag tgattcctaa cccaaacatg cgtccggttg
    12961 cttcactcaa gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
    13021 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
    13081 cgactttcag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
    13141 aaacggcaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
    13201 tactgtagtg ctgcaaggtt tgatttaaac tactggcatg tggagaacgc atcaccgcac
    13261 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
    13321 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaag atatacccac
    13381 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
    13441 ttaacgcaaa aacccccacg ctttgtaccg aaattgcaat cagcagggcg taaaacatgc
    13501 gattattttt cgcgatatac atatcgcgaa aaccacctgt caggcaaaaa cgcccacgtt
    13561 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taata
//
LOCUS       DAZGBC010000132        13534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGBC010000132 DAZGBC010000000
VERSION     DAZGBC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.133"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1336)
                     /gene="plaP"
                     /locus_tag="ACHK1J_003745"
     CDS             complement(<1..1336)
                     /gene="plaP"
                     /locus_tag="ACHK1J_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO7912962.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPV"
     gene            complement(1326..1388)
                     /gene="yoeI"
                     /locus_tag="ACHK1J_003746"
     CDS             complement(1326..1388)
                     /gene="yoeI"
                     /locus_tag="ACHK1J_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO7912963.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1603..2532)
                     /gene="yeeY"
                     /locus_tag="ACHK1J_003747"
     CDS             complement(1603..2532)
                     /gene="yeeY"
                     /locus_tag="ACHK1J_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7912964.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANCAIAGVYAKSDDGKSAI"
     gene            complement(2578..3402)
                     /gene="yeeZ"
                     /locus_tag="ACHK1J_003748"
     CDS             complement(2578..3402)
                     /gene="yeeZ"
                     /locus_tag="ACHK1J_003748"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7912965.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            3829..3879
                     /gene="hisL"
                     /locus_tag="ACHK1J_003749"
     CDS             3829..3879
                     /gene="hisL"
                     /locus_tag="ACHK1J_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO7912966.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    3855..3978
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4025..4924
                     /gene="hisG"
                     /locus_tag="ACHK1J_003750"
     CDS             4025..4924
                     /gene="hisG"
                     /locus_tag="ACHK1J_003750"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO7912967.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            4930..6234
                     /gene="hisD"
                     /locus_tag="ACHK1J_003751"
     CDS             4930..6234
                     /gene="hisD"
                     /locus_tag="ACHK1J_003751"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO7912968.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSTLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6231..7301
                     /gene="hisC"
                     /locus_tag="ACHK1J_003752"
     CDS             6231..7301
                     /gene="hisC"
                     /locus_tag="ACHK1J_003752"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO7912969.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLMAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7301..8368
                     /gene="hisB"
                     /locus_tag="ACHK1J_003753"
     CDS             7301..8368
                     /gene="hisB"
                     /locus_tag="ACHK1J_003753"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO7912970.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8368..8958
                     /gene="hisH"
                     /locus_tag="ACHK1J_003754"
     CDS             8368..8958
                     /gene="hisH"
                     /locus_tag="ACHK1J_003754"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO7912971.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            8958..9695
                     /gene="hisA"
                     /locus_tag="ACHK1J_003755"
     CDS             8958..9695
                     /gene="hisA"
                     /locus_tag="ACHK1J_003755"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO7912972.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSAEDVAALLEAGVARVVVG
                     STAVKSPDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            9677..10453
                     /gene="hisF"
                     /locus_tag="ACHK1J_003756"
     CDS             9677..10453
                     /gene="hisF"
                     /locus_tag="ACHK1J_003756"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO7912973.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDEGRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10447..11058
                     /gene="hisIE"
                     /locus_tag="ACHK1J_003757"
     CDS             10447..11058
                     /gene="hisIE"
                     /locus_tag="ACHK1J_003757"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO7912974.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPKALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGETAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11155..12132)
                     /gene="wzzB"
                     /locus_tag="ACHK1J_003758"
     CDS             complement(11155..12132)
                     /gene="wzzB"
                     /locus_tag="ACHK1J_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO7912975.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETAEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12279..13445)
                     /gene="ugd"
                     /locus_tag="ACHK1J_003759"
     CDS             complement(12279..13445)
                     /gene="ugd"
                     /locus_tag="ACHK1J_003759"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO7912976.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3407 a         3521 c         3462 g         3144 t
ORIGIN      
        1 gaaccgggtt acggaagctc ttggtgacgc aagccaggta aatcagaccg atagcagccc
       61 agatcagacc cagaaccatt gagctttctt ccaggttaac ccacagcgca ccaacggtca
      121 gcgcaccaca catcggcagg aacagatact ggaagtgatc tttcagcgtc ttattacgct
      181 tctcacggat ccagaactgc gagatgaccg acaggttaac gaaggtgaac gccaccaacg
      241 caccaaagtt gatcagcgcc gtagccatca ccaggtcgaa gttgattgcc agcagggcaa
      301 tcgcgccaac caggatgatg ttcatcgccg gagtacgcca tttcgggtgt acataaccga
      361 agaagctttt cgggaatacg ccgtcacgac ccattacgta catcagacgc gctacgcctg
      421 cgtgcgccgc cataccggac gccagtacgg taatggtgga gaagatcagc gcgccaacct
      481 ggaacgcttt acccgcaacg tacagcatga tttcaggctg tgacgcatcc ggatctttaa
      541 agcgagagat atccgggaag tacagctgca ggaagtaagt tgcaaagatg aagatcatgc
      601 cgccgatcag cgcggtcagg aaaatcgcac gcgggatcac gcgctctgca tctttggttt
      661 cttccgacag gttgctgatg ccgtcaaagc cggtaaagga gaagcacagg attgtcgccc
      721 cggtaatcat cgggataaca tgcgcatcgc cagaccagaa tggacgagtg ctcgccagcg
      781 taccagcgcc ttcaccttca aatacgccat aaacaaccat gcccagaatc accgcgatca
      841 gcactacctg caacacgacg attacagtgt tgaagttcgc tacggatttc agactacgca
      901 ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa catccacgat gggatggaag
      961 gcaccagagc ttcaaaatag attttcgcca gcagaatgtt gatcatcggc gcgaacagat
     1021 agtcgagcag agaagaccaa cccaccataa agccgacagt cgggctaatg gatttctggg
     1081 cgtaagtata tgcagagcca gcagaaggat agcggcgaac cagcttcccg tagctcagag
     1141 ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt cggcacatga ccatccgtga
     1201 ggcctgaaac gataccaaaa gtatcaaaca gcgtcatcgg ctgcatatag gcaagaccca
     1261 tcattacaac cggaactaac gtaagcgttt tacgcaattc cacgcgagag gtgtttggag
     1321 taacgttatg cgacatggtc attctccttt acggtgataa ccgtcgcgta agcaaaaaat
     1381 tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta aatatctatc
     1441 cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa taaccgacgc
     1501 tgatgaaacg tcggttttta gtcatttttt gacagcggcg cattgtgcct aaatgggggg
     1561 gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttaaatgg ctgattttcc
     1621 gtcatcagat tttgcataaa caccggcgat cgcacaattt gctaaaattt catcccgcca
     1681 ccagccactg gcaagtcctg cggtctgttc attccagcca atccagacgg gttcaaagag
     1741 agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac gttgcgccag
     1801 ataacggggt aaatagccgc agccgaggcc gctaatttgc agctccagct tggttttaaa
     1861 atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag aagcgcctgc
     1921 ctgcgcagtg tcccccacca caatcgcccg atatcgttta atgatacgac ggtttaacgg
     1981 ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt ccagatcgcc
     2041 caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc caacaataat
     2101 atccgctcgc ccctgagtca gggcatccca ggaaccaccg agtacgccat tgataaactt
     2161 caggcgcgtc acgctatgat gttgataaaa agcttcaatc agtggcgcaa gcagagaaaa
     2221 aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat gcagtttgat
     2281 cgcctgcttt tccagttccc gcacggtatg cagaacttcc cgccctttct ctaataacat
     2341 ttttccggtg cgggtgaatt tagcgcggtg accgctacga tcaagcaatt gaatattaag
     2401 gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt agagtttggc
     2461 cgacgccgcc gcaaaactgc cttctttttc taaggcatcg aggatcatca acacatccag
     2521 cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg tgatcgttta
     2581 ctccagcggc attaccagcg gatcgggata ctggtattca aatcccagtt cattacaaat
     2641 ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga actgcggagg
     2701 ttccagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag ggtgagctgg
     2761 cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag tgatagcgcc
     2821 aataacatct tctaaatgga ctaaattaac accatgttca ccatcaggcg cggtttttcc
     2881 ggcaaagaag cgtccgggat gacgccccgg tccgaccagg cccgcaagac gcagaatatc
     2941 gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata ctcgtccact
     3001 gttggttacc ggattacgcg gtgtagtttc tttcaccgtg ccttgcgcat cgccatagac
     3061 agatgttgag ctggtaaaaa taatgcgggg aatacgatga gccagcgcgc tatccactaa
     3121 ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacgtg ccggaagcgt
     3181 aatgaccagc gcatcggcat ccatcagggc atccagatcg tcagaatcgc aaactaactc
     3241 aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt cgacaccatc
     3301 ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca tcgccagcgg
     3361 catgcctaac caccctaacc cgacaattgc gacctttttc atccgttatc tcctgataca
     3421 ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct atgaatgaaa
     3481 tcgaaacggt tagcaaatat accattgcct gatgcgctcc gcttatcagt cctacaaaaa
     3541 catcaatata ttgaatttag gcaacatttg tagaccagat atggcattca cgccgcatcc
     3601 ggcaggaaca aagcccactt tagttacaga acacaatgtt cttgagggtt ttaatgacat
     3661 attttcacga ttactttttt aacctagtca ctcttagccc cctcctttcc ccgctcattc
     3721 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
     3781 ggtttaggtt aaaagacatc agttgaataa acattcacag agacttttat gacacgcgtt
     3841 caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg
     3901 gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc
     3961 ttccgggggc ttttttattg cgcggttgat aacggttcag acaggtttaa agaggaataa
     4021 gaaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga
     4081 tgactcacgc gaattgctgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct
     4141 gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg acgacattcc
     4201 cggtctggta atggacggcg tggtagatct tgggattatc ggtgaaaacg tgctggaaga
     4261 agagctgctt aaccgtcgcg cccagggtga agatccacgc tactttaccc tgcgtcgtct
     4321 ggatttcggc ggctgccgtc tttcgctggc aacgccggtt gatgaagcct gggacggccc
     4381 gctctcctta aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct
     4441 cgaccagaaa ggcatctctt ttaaatcctg cttactgaac ggttctgttg aagtcgcccc
     4501 gcgtgccgga ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc
     4561 taacggcctg cgcgaagtcg aagttatcta ccgctcgaaa gcttgcctga tccagcgcga
     4621 tggcgaaatg gaagaatcca aacagcaact gatcgacaaa ctgctgaccc gtattcaggg
     4681 tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgtctgga
     4741 tgaagtcatc gccctgctgc caggtgccga acgcccaact attctaccgc tagcgggtga
     4801 tcaacagcgc gtagcgatgc acatggtcag tagcgaaacc ctgttctggg aaacgatgga
     4861 aaaactgaaa gcgctgggtg ccagttcgat tctggtcctg ccgattgaga agatgatgga
     4921 gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa
     4981 cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa gcattacccg cactgttaac
     5041 gatattctcg ataacgtgaa agcacgcggt gatgacgccc tgcgggaata cagcgcgaag
     5101 tttgataaaa ccacggttac cgcgctgaag gtgtctgctg aggaaattgc cgccgccagc
     5161 gaacgcctga gcgacgagct aaaacaggcg atggcggtgg cagtaaagaa tattgaaacc
     5221 ttccacactg cgcaaaaact gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc
     5281 cagcaggtca cgcgcccggt cgcttcagtt gggttgtata ttcctggcgg ctccgccccg
     5341 ctcttctcaa cggtattaat gctggcgact ccggcgcgta ttgcgggctg taaaaaagtg
     5401 gtgctgtgct caccgccgcc gattgccgat gagatccttt atgcggcgca gttgtgcggt
     5461 gtgcaggacg tgtttaacgt cggcggcgca caggccattg ccgcgctggc gtttggtacg
     5521 gaatctgtgc cgaaagtgga caaaatcttc gggccaggaa acgcctttgt caccgaggca
     5581 aaacgtcagg tgagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg
     5641 gaagtgttgg tcattgccga cagcggcgca acgccggatt tcgtggcttc tgatttgctt
     5701 tctcaggctg aacacggccc ggactcacag gtgattttac tgacgcccgc tgctgatatg
     5761 gcgcgtcgcg ttgccgaagc tgtcgaacgc cagttggcag aactgccgcg tgccgaaacc
     5821 gcccgtcagg cactgagcgc cagccgcctg atcgtgacta aagatttagc gcagtgcgta
     5881 gagatctcca accagtacgg cccggagcac ctgatcatcc agacccgcaa cgcccgcgat
     5941 ctggtcgatg gcatcaccag cgccggttca gtatttcttg gtgactggtc accggaatcg
     6001 gcaggtgatt acgcctccgg caccaaccac gttctgccga cttacggtta caccgccacc
     6061 tgttccagcc tcgggctggc ggatttccag aagcggatga ccgtgcagga actgtcgaaa
     6121 gaaggctttt ctacgctggc ttcaaccatt gaaacactgg ccgccgccga gcgcctgacc
     6181 gcccataaaa atgccgttac tttgcgtgtt aacgccctta aggagcaagc atgagcaccg
     6241 tgactattac cgatttagcg cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc
     6301 gtcgtctggg cggtaacggc gacgtctggc tgaacgccaa cgaatacccc acagccgtgg
     6361 agtttcagct tactcagcaa acgctcaacc gctacccgga atgccagccg aaagcggtga
     6421 ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agccgtggcg
     6481 cggacgaagg tattgaacta ctgattcgcg ctttttgcga accgggtaaa gacgccatcc
     6541 tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc tgaaaccatt ggcgtcgagt
     6601 gccgcacagt gccgacgctg gaaaactggc aactggactt acagggcatt tccgacaagc
     6661 tggacggcgt aaaagtggtc tatgtttgca gccccaacaa cccaaccggg caactgatca
     6721 atccgcagga ttttcgcacc ctgctggagt taacgcgcgg taaggcgatt gtggttgccg
     6781 atgaagccta tatcgagttt tgcccacagg catcgctggc tggctggctg gcggaatatc
     6841 cgcacctggc tattttgcgc acactgtcga aagcttttgc tctggcgggg cttcgttgcg
     6901 gatttacgct ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc gccccctacc
     6961 cgctctcgac gccggttgcc gacattgcag cccaggcgtt aagcccgcag gggatcgtcg
     7021 ccatgcgcga acgagtggcg caaattattg ctgaacgcga atacctgatg gccgcactga
     7081 aagagatccc ctgcgtggag caggttttcg actccgaaac caactacatt ctggcgcgct
     7141 ttaaagcctc cagcgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc
     7201 agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa
     7261 gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt
     7321 tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg tggaccgttt
     7381 tgacaaactc gcctttgaac cgggcgtgat cccggaactg ctgaagctgc aaaaagcggg
     7441 ctacaaactg gtgatgatca ctaatcagga tggtctggga acacaaagtt tcccgcaggc
     7501 ggattttgat ggaccgcaca acctgatgat gcagatcttc acctcgcaag gcgtgcagtt
     7561 tgatgaagtg ctgatttgtc cgcacctgcc cgcagatgag tgcgactgcc gtaaaccgaa
     7621 agtaaaactg gtggagcgtt atctcgctga gcaagcgatg gatcgtgcca atagttatgt
     7681 gattggcgat cgcgcgaccg acattcaact ggcggaaaac atgggcatta atggtttacg
     7741 ctacgaccgc gaaaccttga actggccgat gattggtgag caactcacta aacgagaccg
     7801 ttatgcccat gtagtgcgca acaccaaaga gacgcaaatt gacgtccagg tgtggctgga
     7861 tcgtgaaggt ggcagcaaga ttaataccgg cgttggcttc tttgatcaca tgctggatca
     7921 gatcgccacc cacggcggtt tccgcatgga aatcaacgtc aaaggcgacc tctatatcga
     7981 cgatcaccac accgtcgaag ataccggcct ggcgctgggc gaagcgttaa aaatggctct
     8041 tggcgacaaa cgcggtattt gccgctttgg ttttgtgctg ccgatggacg aatgccttgc
     8101 ccgctgcgcg ctggatatct ctggtcgccc gcacctggaa tataaagctg agtttaccta
     8161 ccagcgggtt ggcgatctca gcactgaaat gatcgagcac ttcttccgtt cgctctctta
     8221 caccatgggc gtgacgctcc acctgaaaac caaaggtaaa aacgatcatc accgtgtaga
     8281 gagcctgttc aaagcctttg gtcgcaccct gcgccaggcc atccgcgtgg aaggcgacac
     8341 cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac cggctgcgcc
     8401 aatttgaact cggtgaagtc agccattgcg cgccacggtt atgaacccaa agtcagccgt
     8461 gacccggacg tcgtgttgct ggccgataaa ctatttttac ccggcgttgg cactgcacaa
     8521 gcagcgatgg atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa
     8581 ccggtgctgg gcatctgctt agggatgcaa ctgctggggc ggcgcagcga agagagcaac
     8641 ggcgtcgact tgctgggcat cattgacgaa gacgtgccga aaatgaccga ctttggtctg
     8701 ccgctgccgc atatgggctg gaaccgcgtt tacccgcagg caggcaaccg cctgtttcag
     8761 gggattgaag atggcgcgta cttttacttt gttcacagct acgcaatgcc agtcaatccg
     8821 tggaccatcg cccagtgtaa ttacggcgaa ccattcactg cggcggtaca aaaagataac
     8881 ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa
     8941 aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc accgtggtgc
     9001 gtctccatca gggcgattac ggcaaacagc gcgattacgg taacgacccg ctgccgcgct
     9061 tacaggatta cgccgcgcag ggtgccgaag tgttgcatct ggtggatctg accggggcaa
     9121 aagatccggc taaacgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgttc
     9181 cggtgcaggt tggcggcggc gtgcgtagcg cagaagacgt ggcggcgtta ctggaagcgg
     9241 gcgttgcgcg cgtggtggtc ggctccaccg cggtgaaatc accagatatg gtgaaaggct
     9301 ggtttgaacg tttcggtgcc gatgccttag tgctggcgct ggatgtccgt attgacgagc
     9361 aaggcaacaa gcaggtggca gtcagcggct ggcaagagaa ctcgggcgtt tcactggaac
     9421 aactggtgga aacctatctg cccgtcggcc tgaaacatgt gctgtgtacc gatatctcgc
     9481 gcgacggcac gctggcaggt tctaacgtct ctttatatga agaagtgtgc gccagatatc
     9541 cgcaggtggc atttcagtca tccggcggca ttggcgacat tgatgatgtg gctgccctgc
     9601 gtggcactgg cgtgcgcggc gtaatagttg gtcgggcatt actggaaggt aaattcaccg
     9661 tgaaggaggc catcgcatgc tggcaaaacg cataattcca tgtctcgacg ttcgtgatgg
     9721 tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccgct
     9781 ggcaaaacgc tacgctgaag aaggcgctga cgaactggtg ttctacgata tcaccgcttc
     9841 cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat
     9901 tccgttctgt gtggcgggtg ggattaaatc gctggatgac gccgccaaaa ttctttcctt
     9961 tggcgcggat aaaatttcca tcaactcccc tgcgctggcg gacccgacgt taattactcg
    10021 cctggcggat cgatttggcg tgcagtgtat tgtggtcggt attgatacct ggtacgacgc
    10081 tgaaaccggt aaatatcatg tgaatcaata taccggcgat gaaggccgca cccgcgtcac
    10141 tcagtgggaa acgctcgact gggtacagga agtgcaaaaa cgcggtgccg gagaaatcgt
    10201 cctcaacatg atgaatcagg acggcgtgcg taacggttac gacctcgaac aactgaaaaa
    10261 agtgcgtgaa gtttgccacg tcccgctgat tgcttccggt ggcgcgggca ccatggaaca
    10321 cttcctcgaa gccttccgcg atgccgacgt tgacggcgcg ctggcagctt ccgtattcca
    10381 caaacaaata atcaatattg gtgaattaaa agcgtacctg gcaacacagg gcgtggagat
    10441 caggatatgt taacagaaca acaacgtcgc gaactggact gggaaaaaac cgacggactt
    10501 atgccggtga ttgtgcaaca cgcggtatct ggcgaagtgt taatgctggg ctatatgaac
    10561 ccgaaagctc tggacaaaac catcgaaagc ggcaaagtca ccttcttctc gcgcactaaa
    10621 cagcgactat ggaccaaagg cgaaacgtcg ggcaatttcc tcaacgtagt aaatattgcg
    10681 ccggactgcg acaacgacac gctactggta ctggcgaatc ctatcggacc aacctgccat
    10741 aaaggcacca gcagctgctt cggcgaaact gctcaccagt ggctgtttct gtatcaactg
    10801 gaacaactgc tcgccgaacg caaatctgcc gacccggaaa cctcttacac tgccaaactg
    10861 tatgccagcg gcaccaaacg cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca
    10921 ctggccgcga ctgtacatga ccgctttgag ctcaccaacg aggcgtctga tttgatgtat
    10981 cacctgctgg tgctgttgca agatcaggat ctggatttaa cgacggtgat tgagaacctg
    11041 cgtaaacggc atcagtgagt tgcggggtaa gcggatgcaa tattgttgtc gcatccggca
    11101 aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt
    11161 cgcgttgtaa ttacgcagag cgttacgccc cagcacaatc cccgcaccaa ccatgccgcc
    11221 cagcagcacc gccagaatca aggtgattgc ctttttcgga ctatcgcgac gaataggtaa
    11281 cgtcggtttc atcacataac ggtaagcatg aatatcaaga tcatcaactt ttaagctttc
    11341 gatatccagc aggttttgac gagtctgata gtagtttggt gagaacacca acggacgggt
    11401 cgcctcatgc ttaatcatcg actccagcgc ttcgctcccc aaaaggaaca acgtatcttg
    11461 tgtcacatct tcagtctgct gaacctgtgg ctttgtcacc tgcgcctgat tcgcatactg
    11521 caacgcttcc tgaatctgac ggatacgcag atctttctgc tcctgcgcga ctacttcctg
    11581 agttttcaga gaatcctgca agtttttcat acgcagcgcg atgttgtctt tcaggtcttt
    11641 ttccagttct tcgtttacct ggtcgtcaac ttgctggata tacttcgcca gctgcttctg
    11701 cgcaccttca gctgtttcac ccacataaga aactgctaaa ggtaacgact ggtttttaac
    11761 ggtaggctca atggtcagct tctctgcttc ttcctggtta tccagcgttt ccgctaatgc
    11821 tgagaatgca gtgctgtaac gtccgataag cgaagcctga atgtcggaga ctttcggtac
    11881 agccgatccg tagatcacat tcatcgcgtt ggtataacca gcgatttgcc ccacatcagg
    11941 ttgagtaacg atagctgtgg aagtccactt ttctttggca acggccagat accctatagc
    12001 cagggcaata gcaacaatga cagagatgat aattgtcatc ttgccgcgcc acaactgcac
    12061 cagtaaatca atcaaatcaa tctgttccgg gtcatggttt tgcccagaaa cattattatt
    12121 ttctactctc atcattaccc taacggacga aataatttta agtcgccata gtttagcgat
    12181 aatcgccatg aatgctatag gataaatgat aaaaataatg aattattaca aagggaacat
    12241 aagagaatca gggcgaaaat cgccctgata acaggatgtt agtcactgcc aaagagatcg
    12301 cgggtgtaga ctttgtctgc cacatcctta agctcttctg ccatacggtt ggaaataatg
    12361 acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca ggcgagagtt gaagaatgag
    12421 tcttctttca ttaccggttc gtaaatgatt acttcaacgc ctttcgcctt gatacgcttc
    12481 ataatcccct gaatggaaga cgcacggaag ttatctgacc cgctcttcat aatcagacga
    12541 taaataccca ccacttgcgg tttgcgtgac aaaatggcat cggcaataaa gtctttacgc
    12601 gtgcggtttg catcgacaat tgccgagatc aggttattcg gcacagactg gtagttcgcc
    12661 agtaactgct tggtatcttt cggcagacaa taaccaccat aaccaaacga cggattgttg
    12721 taatggttgc caatgcgtgg gtcgagacaa acgccttcga taatctggcg agtattcagc
    12781 cctaaacttt ctgcatagct atccagttca ttaaagtatg ctacgcgcat cgccagatag
    12841 gtattagcaa acagtttaat cgcttctgct tcggttgaat cggtgaacag ggtcgggata
    12901 ttttgcttaa tcgcgccttc ctgtaataac gcagcaaaac gttctgcgcg ttccgaacgc
    12961 tcaccgatta caatacgtga cggatgaagg ttatcgtaaa gagctttacc ttcacggaga
    13021 aattccggag agaaaataag gttatcgata cccagacgtt ctttaattga tttggtgaaa
    13081 cctaccggaa ttgtagattt aatcaccatt accgcgttgg gattaatttc tgtcacatcg
    13141 cgaatgaccg cttccacgct tgaggtatta aaataatttg ttttcgggtc ataatcggta
    13201 ggcgtggcaa taataacgta atcagcattt ttatacgcgt catacttatc tgtcgtagcg
    13261 cggaaattga gatctttagt cgccagatac tcttcaatct ccttatcaac aatcggtgac
    13321 tgcttcttgt taagcatgtc aactttggcc tgaacgatat ccagtgcaac cacttcgtgg
    13381 ttttgcgcaa tcagaatacc atttgaaaga ccaacataac ctgttcctga aattgttatt
    13441 ttcatagtcg tttagcttag caatattaac ttgtatagtt atatgtacag ttgattagga
    13501 aagttgtcaa tataacagta ccaaaaaaac ctta
//
LOCUS       DAZGBC010000133        13462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGBC010000133 DAZGBC010000000
VERSION     DAZGBC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.134"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..1751
                     /gene="mqo"
                     /locus_tag="ACHK1J_003760"
     CDS             105..1751
                     /gene="mqo"
                     /locus_tag="ACHK1J_003760"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO7912977.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(2127..2615)
                     /gene="eco"
                     /locus_tag="ACHK1J_003761"
     CDS             complement(2127..2615)
                     /gene="eco"
                     /locus_tag="ACHK1J_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO7912978.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(2749..2913)
                     /locus_tag="ACHK1J_003762"
     CDS             complement(2749..2913)
                     /locus_tag="ACHK1J_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7912979.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            3022..3516
                     /gene="napF"
                     /locus_tag="ACHK1J_003763"
     CDS             3022..3516
                     /gene="napF"
                     /locus_tag="ACHK1J_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO7912980.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3506..3769
                     /gene="napD"
                     /locus_tag="ACHK1J_003764"
     CDS             3506..3769
                     /gene="napD"
                     /locus_tag="ACHK1J_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO7912981.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNASPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3766..6252
                     /gene="napA"
                     /locus_tag="ACHK1J_003765"
     CDS             3766..6252
                     /gene="napA"
                     /locus_tag="ACHK1J_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO7912982.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            6259..6954
                     /gene="napG"
                     /locus_tag="ACHK1J_003766"
     CDS             6259..6954
                     /gene="napG"
                     /locus_tag="ACHK1J_003766"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO7912983.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6941..7804
                     /gene="napH"
                     /locus_tag="ACHK1J_003767"
     CDS             6941..7804
                     /gene="napH"
                     /locus_tag="ACHK1J_003767"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO7912984.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7801..8250
                     /gene="napB"
                     /locus_tag="ACHK1J_003768"
     CDS             7801..8250
                     /gene="napB"
                     /locus_tag="ACHK1J_003768"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO7912985.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            8260..8862
                     /gene="napC"
                     /locus_tag="ACHK1J_003769"
     CDS             8260..8862
                     /gene="napC"
                     /locus_tag="ACHK1J_003769"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO7912986.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8875..9498
                     /gene="ccmA"
                     /locus_tag="ACHK1J_003770"
     CDS             8875..9498
                     /gene="ccmA"
                     /locus_tag="ACHK1J_003770"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO7912987.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            9495..10157
                     /gene="ccmB"
                     /locus_tag="ACHK1J_003771"
     CDS             9495..10157
                     /gene="ccmB"
                     /locus_tag="ACHK1J_003771"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO7912988.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            10199..10936
                     /gene="ccmC"
                     /locus_tag="ACHK1J_003772"
     CDS             10199..10936
                     /gene="ccmC"
                     /locus_tag="ACHK1J_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO7912989.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10933..11142
                     /gene="ccmD"
                     /locus_tag="ACHK1J_003773"
     CDS             10933..11142
                     /gene="ccmD"
                     /locus_tag="ACHK1J_003773"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO7912990.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRSVAQQRAREARLRAAQQQEAA"
     gene            11139..11618
                     /gene="ccmE"
                     /locus_tag="ACHK1J_003774"
     CDS             11139..11618
                     /gene="ccmE"
                     /locus_tag="ACHK1J_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO7912991.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11615..>13462
                     /gene="ccmF"
                     /locus_tag="ACHK1J_003775"
     CDS             11615..>13462
                     /gene="ccmF"
                     /locus_tag="ACHK1J_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO7912992.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAG"
BASE COUNT         2853 a         3428 c         3967 g         3214 t
ORIGIN      
        1 atataattta taaacattat ttaaatgttg ttacttaagt gttaccgttg atgccgcgca
       61 aatctcacct gcaataaagc gactaaaagt aaggcattaa caagatgaaa aaagtgactg
      121 ccatgctctt ctcgatggcc gtggggctta atgccgtttc gatggcggca aaagcgaaag
      181 cgtccgagga gcaggaaact gatgtactgt tgattggcgg cggcattatg agcgccacgt
      241 tggggaccta tttacgcgag ctggagcctg aatggtcgat gaccatggtg gagcgcctgg
      301 agggggtcgc gcaggagagt tcgaacggct ggaacaacgc cggaaccggg cattctgcac
      361 tgatggaact gaactacacc ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag
      421 tcgccattaa cgaagcattt cagatttccc gccagttctg ggcgcaccag gtcgagcgcg
      481 gcgtgctgcg tactccgcgt tcatttatca ataccgttcc gcatatgagc tttgtctggg
      541 gcgaggataa cgtcaatttc ctgcgcgccc gttacgccgc gttgcaacaa agctcgctat
      601 ttcgcggtat gcgttactct gaagatcacg cgcagatcaa agagtgggca ccgttagtga
      661 tggaagggcg cgatccgcaa cagaaagtgg cagccacgcg tacggaaatt ggtaccgatg
      721 tgaactacgg cgagatcacc cgccagttaa ttgcttcctt gcagaagaaa tctaacttct
      781 cgctgcaact cagcagcgaa gtccgcgccc taaagcgtaa tgacgataac acctggaccg
      841 ttaccgttgc cgatctgaaa aatggcactg cacagaacat ccgtgccaaa tttgtcttta
      901 tcggcgcggg cggtgcggca ctgaagctgt tacaggaatc ggggattccg gaagcgaaag
      961 actacgccgg tttcccggtg ggcggtcagt tccttgtttc ggaaaacccg gacgtggtta
     1021 atcaccatct ggcgaaggtt tacggtaaag catccgttgg cgcaccaccg atgtcggttc
     1081 cgcatatcga tacccgcgtt ctggacggta aacgcgtagt gctgtttggg ccatttgcca
     1141 ccttctcaac caaattcctc aaaaacggtt cattgtggga tctaatgagt tccaccacca
     1201 cctctaacgt gatgccgatg atgcacgtcg ggctggataa tttcgatctg gtgaaatatc
     1261 tggtgagtca ggtgatgttg agtgaagagg atcgttttga agcgttgaaa gagtactatc
     1321 cgcaagcgaa aaaagaggac tggcgtttgt ggcaagcggg gcagcgtgtg cagattatca
     1381 agcgtgatgc cgataaaggt ggtgtactgc gtctgggtac tgaagtggtc agtgaccagc
     1441 aagggaccat tgccgcgctc ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta
     1501 tgttggatct gctggaaaaa gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta
     1561 cgttgaaagc gatcgttccg tcttatggac gcaagctgaa cggtgatgta gccgcaacag
     1621 agcgcgagtt gcagtacacc agcgaagtgc tggggctgaa atacgacaaa ccgcaagcag
     1681 cagatagtac gccgaaaccg cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata
     1741 ttgcgttgta atgatgtgcc acatccggca tggtatgccg gatgtggcgt atgctgataa
     1801 gacgcgccag cgtcgcatca ggcaaccggc tcgggcgtta gatgttggat gcggcgtaac
     1861 cgccttatcc gacctacggg gtggagcact tcacagcggt gtaggcctga taagacgcgt
     1921 cagcgtcgca tcaggcaacc ggctcggacg ttggatgtcg gatgcggcgt tcccgcctta
     1981 tccgacctac ggggtggagc acttcacagc ggtgtaggcc tgataacacg cgtcagcgtc
     2041 gcatcaggca accggctcgg gcgttagatg ttggatgcgg cgtaaccgcc ttatccaacc
     2101 tatggggtgc cgcacttcac ggcagtttag cgaactaccg cgttgtcaat tttctcttcc
     2161 gccttccaga cgcggtactt cacatctaca ttgtctggcg tatacaccac gatcggcagc
     2221 ttgctgttgt aacgcagcat tccagcatcg cccagatacg cggtgacaaa tttcttctct
     2281 ttcttgccat ccgggcaggc catcatcgtg gaaaccggcg agctaacttt atcaaagaca
     2341 taataatcat agccccagcc ttccagcgtt ttgctttcca gcttcccgcc gagacgatgc
     2401 aaattgcaat cgacttccag cgtctgaccg attaacagtt ctactttcag ggtagattca
     2461 tcttcttgcg gggttaactg aatcacctga cgcttcatgc ctttttcagc ttgtggataa
     2521 ggcgcgattt tttccagtgg ctggacgctc tctgccgccc aggcggaagt ggtggcgaaa
     2581 gcggcaaaca atactgcagg tagaatggtc ttcatttttt gctttccttc ttcatttata
     2641 ggtttgcacc gatgatgtcg ctgaaggtta acatattcat cagatttatt aatttgcttt
     2701 atgaataact taaagaatat tcagacaatt ttacctgctc ttttagtgtt aaattctgat
     2761 gagagaacaa accagcagaa atatctctgg tgaattaatc gctatataaa tatgtttata
     2821 accatttgaa atgtgagcaa aagcccgttt ttgccacgct tcccgccact cttttgatcc
     2881 tgctacaggt tttacctcga tcggggtatg catctttgac acatccttta atatcttagc
     2941 ggctataaaa atggcttatt aattatgcgg tttatttggt cgctctcaat tttcagagcg
     3001 cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc gtcggggcat actcactggt
     3061 cgctggcgca aagccagtaa cggtatccgt ccgccctggt ccggtgatga atctcatttt
     3121 ctgacccatt gcacccgctg cgacgcctgt attaatgctt gtgaaaacaa cattctgcaa
     3181 cgcggcgcgg gcggctatcc gagcgttaat ttcaaaaata acgagtgcag cttctgttac
     3241 gcctgcgcgc aggcttgccc cgaatcgcta ttttctccgc gccacaccag ggcctgggat
     3301 ttgcagttca ccattgggga cgcgtgcctg gcgtatcagt cagttgaatg ccgccgctgt
     3361 caggatagct gtgagccaat ggcaattatc tttcgcccga cgctctccgg gatctaccag
     3421 ccgcaactta atagccaact ttgcaacgga tgtggagcat gtgcagccag ctgtccggta
     3481 tcagccataa ctgcggagta tctccatgca cactaactgg caagtttgca gcctggtcgt
     3541 gcaggccaaa agcgaacgaa tttcagacat cagcacccaa ctgaacgcct ctcccggctg
     3601 tgaagttgct gtcagcgacg cgccgagcgg tcagttgatt gtggtggtgg aagcagaaga
     3661 cagcgaaacg ctgatccaaa ccattgagtc agtacgcaac gtagagggcg tgctggcggt
     3721 gtcgctggtt tatcaccagc aggaagagca aggtgaggaa acaccatgaa actcagtcgt
     3781 cgtagcttta tgaaagctaa cgccgttgcg gccgctgcgg cggctgccgg tctcagcgtg
     3841 ccgggcgttg cccgcgccgt tgttggtcag caggaagcca tcaaatggga taaagcgccg
     3901 tgccgtttct gcggtactgg ttgcggcgtt ctggtcggaa cgcagcaggg gcgtgtggtg
     3961 gcctgtcagg gcgacccgga cgcaccggtt aaccgtggcc tgaactgcat taagggctat
     4021 ttcctgccca agatcatgta cggtaaagac cgtttgacgc agccgctgct gcgtatgaaa
     4081 aacggtaaat atgacaaaga aggcgaattt accccaatca cctgggatca ggccttcgat
     4141 gtgatggaag agaaattcaa aaccgccctg aaagaaaaag ggccggaatc gatcggtatg
     4201 ttcggttctg gtcagtggac tatctgggaa ggttatgccg catccaagct gtttaaagcg
     4261 ggcttccgtt cgaacaacat cgacccgaac gcgcgtcact gtatggcgtc ggcagtagtt
     4321 ggctttatgc gtacctttgg tatggatgag ccgatgggct gctatgacga catcgagcag
     4381 gctgacgcgt ttgtgctgtg gggcgcaaac atggcggaga tgcacccgat cctctggtca
     4441 cgcatcacta accgtcgtct ctctaaccag gacgtcaccg tggcggtgct ttcaacctac
     4501 cagcatcgta gcttcgagct ggcggataac ggcatcatct ttacgccgca atctgacctg
     4561 gtgatcctga actacatcgc caactatatc attcaaaaca atgcgataaa tcaggacttc
     4621 tttagcaagc acgttaacct gcgcaaaggg gcgacggaca tcggctacgg tttacgtccg
     4681 acccatccgc tggaaaaagc agcgaagaat ccgggttctg acgcctccga accgatgagc
     4741 tttgaagatt acaaagcctt cgttgctgag tatacgctgg aaaaaaccgc tgaaatgact
     4801 ggcgtaccaa aagatcagct ggaacaactg gcgcagctgt atgccgatcc gaacaagaaa
     4861 gtcatctcct actggacgat gggcttcaac cagcatactc gtggcgtgtg ggccaacaac
     4921 ctggtctaca acctgcacct gctgaccggc aaaatttccc agccgggttg cggtccgttc
     4981 tccctgaccg ggcagccttc cgcgtgtggt actgcgcgtg aagtgggcac ctttgctcac
     5041 cgtctgcctg cggacatggt ggtaactaac gagaaacatc gtgatatctg cgagaagaag
     5101 tggaatatcc cgagcggcac cattccggcg aaaatcggtc tgcatgcggt agcacaagac
     5161 cgtgcgctga aagacggcaa gctgaatgtt tactggacca tgtgtaccaa caacatgcag
     5221 gccgggccga acattaatga agagcgtatg ccgggctggc gcgatccgcg caacttcatc
     5281 atcgtctccg atccgtatcc gacagtcagt gcgctggccg ccgacttgat cctgccgacc
     5341 gcaatgtggg tagaaaaaga gggggcttac ggtaacgccg aacgccgtac tcagttctgg
     5401 cgtcagcagg tacaggcgcc aggcgaagcg aaatcggacc tctggcagtt agtccagttc
     5461 tcccgccgct tcaaaactga agaagtatgg ccggaagagc tgctggcgaa gaaaccggaa
     5521 ctgcgtggca aaacgctgta cgaagttctg tatgccaccc ccgaagtgag caaattcccg
     5581 gtatccgaac tggcggaaga tcagctgaac gatgaatccc gcgagctggg cttctatctg
     5641 caaaaagggc tgttcgaaga gtacgcatgg tttggtcgcg gtcacggtca cgatctcgcg
     5701 ccgttcgatg actaccacaa agcgcgcggt ctgcgctggc cggtggtgaa cggtaaagaa
     5761 acgcagtggc gttacagcga aggtaacgac ccgtacgtga aagcgggcga aggctacaag
     5821 ttctacggta aaccggatgg caaagcggtg atcttcgcac tgccgttcga accagcggcg
     5881 gaagcaccgg atgaagagta cgacctgtgg ctctctaccg gacgcgtcct ggagcactgg
     5941 cacaccggca gtatgactcg ccgtgtgccg gaactgcacc gtgccttccc ggaagcggtc
     6001 ctgtttattc acccgctgga tgcgaaagcg cgcgatctgc gccgtggcga caaagtgaaa
     6061 gtggtttctc gccgtggcga agtgatctcg attgtcgaaa cgcgcggtcg taaccgtccg
     6121 ccgcagggcc tggtgtacat gccgttcttc gacgccgcac agctggttaa caaactaacg
     6181 ctggacgcga ccgatccgct ctcgaaagag acggacttca agaagtgcgc ggtcaaactg
     6241 gagaaggtgt aagccattat gtcccggtca gcgaaacctc aaaatggtcg ccgccgcttt
     6301 ctgcgcgatg tcgttcgcac agcaggcggg ctggctgccg tgggtgtggc gctggggtta
     6361 caacagcaaa ccgcacgcgc atctggcgtg cggttgcgcc cgcccggagc cataaacgag
     6421 aacgcctttg ccagtgcttg tgtgcgttgc ggtcagtgtg ttcaggcttg cccttacgac
     6481 accttaaaac tggcgacgct ggcctctggc ctgtcggcgg gcacgccata ttttgtcgca
     6541 cgggatattc cttgcgaaat gtgtgaggac attccgtgcg ccaaagtgtg cccgagcggt
     6601 gcgctggatc gtgagattga atcgatcgac gacgcgcgga tggggctggc ggtactggtg
     6661 gaccaggaaa actgtctcaa ctttcagggg ctgcgctgcg atgtttgtta tcgcgaatgc
     6721 ccgaaaattg atgaggccat caccctggag ctggagcgca acacgcgtac cggtaagcac
     6781 gcccgctttc tgccgacggt tcacagcgac gcctgtaccg gttgcggtaa gtgcgaaaaa
     6841 gtgtgcgtgc tggaacaacc ggcaatcaag gtgttaccgc tgtcactggc gaaaggggag
     6901 ttaggccacc attaccgctt cggctggctg gaggggaaca atggcaaatc gtaaacgtga
     6961 cgccgggcgc gaggcgctgg agaaaaaagg ctggtggcgc agtcaccgtt ggctggtgtt
     7021 acgtcgcctt tgtcagttct tcgtgctggg gatgtttttg agtggtccgt ggtttggtgt
     7081 gtggatcttg cacggcaact acagcagtag cctgttattc gacaccgttc cgctgaccga
     7141 tccgctgatg acgctgcaaa gtcttgccag tggtcatctg cctgccacgg tggcgttgac
     7201 cggtgcggtg attatcaccg tgctctacgc cctggcagga aagcgattat tttgcagctg
     7261 ggtctgcccg ctgaacccga ttaccgactt agcgaactgg ttacgcaggc gctttgacct
     7321 caatcagtct gcgacgatcc ctcgccacat tcgctacgtg ctgttggtgg tcattctggt
     7381 gggatcagcc ttaactggca cgctcatctg ggaatggatc aaccccgttt ccctgatggg
     7441 gcgtagcctg gtaatgggct tcggcagcgg cgcgctgctt attctcgcgc tgtttttatt
     7501 tgatctactg gtcgttgaac acggctggtg cgggcacatt tgtccggttg gcgcgctgta
     7561 tggcgtgctg ggtagcaaag gtgtgattac cgttgcggcc agcgatcggc agaaatgtaa
     7621 ccgctgtatg gattgttttc atgtttgccc ggaaccgcat gtgctacgtg ccccggtgct
     7681 ggatgaacaa agcccggtgc aggtcaccag ccgcgattgc atgacgtgcg gtcgctgcgt
     7741 ggatgtctgt tctgaggatg tatttacaat aactacacga tggagttcgg gagcgaaatc
     7801 atgaaaagcc atgacctgaa gaaagcgctg tgtcaatgga cggcgatgct ggccctggtg
     7861 gtaagcggcg cggtttgggc ggctaatggc gtcgatttta gccagtcgcc ggaagtatcc
     7921 gggacgcagg aaggggccat tcggatgcca aaagagcagg atcggatgcc gctgaactat
     7981 gtgaatcagc cgccaatgat cccgcatagc gttgaaggtt atcaggtaac gaccaatacc
     8041 aaccgctgct tgcagtgcca cggtgtcgaa agctatcgca ccactggcgc gccgcgtatc
     8101 agtcctactc actttatgga cagcgacggc aaagtgggcg cggaagtggc tccgcgtcgt
     8161 tatttctgtc tgcaatgtca cgtaccgcag gccgataccg cgccaatcgt ggggaatacc
     8221 tttaccccat caaaaggtta cgggaaataa gaggttatta tgggaaattc tgaccgtaag
     8281 cctggtctga ttaagcgcct gtggaaatgg tggcgtaccc ccagccgtct ggcgctgggg
     8341 acgctgctgt tgatcggttt tgtcggcggc atcgtcttct ggggcggctt taacaccggg
     8401 atggaaaaag ccaataccga agagttctgc attagctgcc acgaaatgcg taacacggtg
     8461 tatcaggaat acatggattc cgtgcactac aacaaccgta gcggcgtccg tgcgacctgt
     8521 ccggattgtc acgttccgca cgagtttgtg ccgaagatga tacgcaagct caaagcaagt
     8581 aaagagctgt atggtaaaat ttttggcgtt attgacacgc cgcagaaatt tgaagctcat
     8641 cgtctgacga tggcacagaa tgagtggcgg cgcatgaagg acaataactc gcaggagtgc
     8701 cgtaactgtc acaacttcga gtatatggat acaaccgccc agaaatcggt tgccgcgaag
     8761 atgcatgacc aggcggtgaa agatgggcaa acctgtattg attgccataa agggatagcg
     8821 cacaagctcc ccgatatgcg tgaagtcgag ccaggttttt aacagggtta ttgcgtgggt
     8881 atgcttgaag tcagagagtt actttgtgag cgggatgaac gaaccttatt tagtggcttg
     8941 tcatttacgc tgaacgcagg agagtgggta caaatcaccg gtagcaacgg cgcggggaag
     9001 acaacgcttc tccgtttgct gacggggttg tctcgccctg acgcaggcga ggttctctgg
     9061 caagggcagc ccttgcatca ggtacgcgac agctaccatc aaaacctgtt atggataggc
     9121 catcagccgg ggatcaaaac ccggctgacg gcgttagaaa atctgcactt ttatcatcgc
     9181 gatggcgata ccgcacaatg tctggaagcc ctggcgcagg ccgggcttgc cggattcgaa
     9241 gatattccgg taaatcaact ttctgccggg caacaacgcc gcgtcgcttt agcgcgtctg
     9301 tggctgaccc gtgccacgtt atggatcctc gacgagcctt ttaccgcgat tgacgttaac
     9361 ggcgtcgatc gtctgaccca gcgtatggcg cagcatacag agcagggggg gattgtgatt
     9421 ctcactaccc accagccgct caacgttgct gaaagtaaaa ttcgccgcat ttcactgacg
     9481 caaacgaggg ccgtatgatg ttctggcgca ttttccgtct tgagctgcgc gtagcgtttc
     9541 gccatagcgc tgaaatcgcc aacccgctgt ggttcttcct gattgtaatt accctttttc
     9601 cgctcagtat cggtccggaa ccgcaactgc tggcacgtat tgcaccgggc attatctggg
     9661 ttgccgcgct gctttcatcc ctgctggcgc tggaacgact gttccgtgac gatttgcagg
     9721 acggcagtct tgaacaattg atgttgttgc cgttaccctt gcccgccgtt gtgctggcga
     9781 aggtgatggc gcactggatg gtaaccggtc tgccgttact catcctttcg cccctggtag
     9841 caatgctact gggaatggat gtttatggct ggcaagtgat ggcgttaacg ctactgctgg
     9901 gaacccctac gcttggcttt cttggtgcgc cgggcgtggc gctgacagtg ggacttaagc
     9961 gcggtggtgt gctgctcagc atactggtgt taccgctgac tatcccatta ctcatctttg
    10021 ccaccgccgc gatggacgcg gcttctatgc atttgcccgt tgacgggtat ctggcaattt
    10081 taggcgcgtt gctggcaggc accgcgacat taagtccttt tgcgacggcg gcagcgttac
    10141 gaatcagcat tcaataacgc tggattactg atttttgttc gagtctggta tcgaaactat
    10201 gtggaaaaca ctgcatcaac tggcgatccc accacggctg tatcaaatct gtggctggtt
    10261 tataccgtgg ctggcaattg ccagtgtggt cgtgcttacc gtcggctgga tctggggatt
    10321 cggctttgct ccggctgatt atcagcaggg aaatagctac cgcattatct acctgcatgt
    10381 gcctgcggcg atctggtcga tgggcattta tgcatcaatg gcggtggcgg cttttattgg
    10441 ccttgtctgg cagatgaaaa tggccaacct ggcggtggcg gcgatggccc ccattggtgc
    10501 cgtgtttacc tttattgccc tggttaccgg ctctgcatgg gggaaaccga tgtggggcac
    10561 ctggtgggta tgggatgcac gtctgacttc tgaactggtg ctgctgtttt tgtatgtggg
    10621 tgtgattgcc ctgtggcacg ccttcgacga ccgccgtctg gcgggccgtg cggcaggtat
    10681 cctggtgctg attggcgtgg tgaatctgcc gattattcat tactccgtgg agtggtggaa
    10741 caccctgcat cagggatcaa cgcggatgca gcaaagtatc gatcctgcga tgcgttcgcc
    10801 gctgcgctgg tcgatttttg gcttcctgct gctgtctgcc acgctgacgc tgatgcggat
    10861 gcgtaatttg attttgctga tggaaaaacg ccgtccgtgg gtgagtgaac tgatactgaa
    10921 aagaggccgt aaatgacccc tgcatttgct tcctggaatg aatttttcgc aatgggcggt
    10981 tacgcctttt ttgtctggct ggcggtggtg atgaccgtta ttccgctggt ggttttggtc
    11041 gtgcactcgg tgatgcagca tcgcgcaatt ctgcgtagcg tggcgcaaca gcgggcgcga
    11101 gaggcgcgtt tacgtgctgc acaacagcag gaggctgcat gaatattcgc cgtaaaaacc
    11161 gcttgtggat tgcctgtgcc gtgttggcag ggctggcgct gactatcggt ctggtgctat
    11221 atgcgctgcg ctcgaatatc gatctctttt atacgccggg ggaaattctc tacggcaagc
    11281 gtgaaactca gcagatgccg gaagtcggtc agcgtctgcg cgttggcggg atggtgatgc
    11341 cgggtagcgt gcagcgcgat cccaattcgc tgaaagtaac gttcaccatt tacgatgctg
    11401 aaggctcagt ggatgtctct tacgaaggca ttttgccgga tctgttccgt gaagggcagg
    11461 gcgttgtggt gcagggcgag ctggaaaaag gcaatcatat cctcgcgaaa gaagtgctgg
    11521 cgaaacacga cgaaaactac acgccgccag aagttgagaa agcgatggaa gctaaccacc
    11581 gtcgcccggc gagtgtttat aaggacccag catcatgatg ccggaaattg gtaacgggct
    11641 gctgtgcctg gcgctaggaa ttgcgctgct gctgtctgtg tatccgctat ggggcgtggc
    11701 gcgcggagat gcgcgcatga tggcgtcttc ccgcttgttt gcctggctgc tgtttatgtc
    11761 tgtagctggc gcatttctgg tgctggtcaa tgctttcgtg gtcaacgact tcaccgtcac
    11821 ctatgttgcc agcaactcca atacccagct tccggtgtgg tatcgcgtgg cggctacctg
    11881 gggtgcacat gaaggctcac tgctgctgtg ggtgctgctg atgagcggct ggacctttgc
    11941 ggtggcgatt tttagtcagc gtattccgct ggatattgtg gcccgcgtac tggcgataat
    12001 ggggatggtc agcgtcggct ttttgctgtt cattctcttt acctctaacc cattctcacg
    12061 cacgctgccg aacttcccga ttgaagggcg cgatcttaac ccgctgttgc aggatccggg
    12121 gctgatcttc catccgcctc tgctctatat ggggtacgtg ggtttctcgg tggcgtttgc
    12181 ttttgccatt gcttctttgt tgagcgggcg tctggacagc acttatgcgc gttttactcg
    12241 tccgtggacg ctggcagcgt ggatcttcct gacgctcggc atcgtgctcg gttccgcatg
    12301 ggcctattac gaactcggct ggggtggctg gtggttctgg gatccggtag aaaacgcctc
    12361 gtttatgccg tggctggtgg ggactgcgtt gatgcactcg ctggcggtga ctgaacagcg
    12421 tgccagcttc aaagcgtgga cattactgct ggcaatcagt gccttctcgt tgtgtctgct
    12481 ggggactttc ctcgtgcgtt ccggcgtgct ggtatcggta cacgcgtttg cgtctgatcc
    12541 ggcgcgcggt atgtttatcc tcgcctttat ggtgctggtg attggcggtt cgctgctgct
    12601 gtttgccgcg cgtggacaca aagttcgctc acgcgtaaac aatgcgctgt ggtcgcggga
    12661 atctttgctg ttagcgaaca acgttttgct ggtcgccgcg atgctggtgg tgttgctggg
    12721 gacgctgctg ccgctggtgc acaaacaact ggggctgggc agtatttcga ttggcgaacc
    12781 gttcttcaac accatgttta cctggctgat ggtgccgttt gcgctactgc ttggtgtcgg
    12841 tcctctggtg cgctgggggc gcgatcgccc acgtaaaatc cgcaatttat tgattatcgc
    12901 cttcatctct acgctggtgc tgtctctgct gttgccgtgg ctgttcgaaa gcaaagttgt
    12961 ggcgatgacg gtgctcggcc tggcaatggc ctgctggatt gcggtgctgg caattgcaga
    13021 agcggcgctg cgtatttcac gcggcacgaa aaccaccttc agctattggg ggatggtggc
    13081 ggctcacctg gggctggcag tgacaattgt tggtattgct ttcagtcaga actacagcgt
    13141 tgagcgtgat gtgcgcatga agtccggcga tagcgtcgat attcatgaat atcgcttcac
    13201 cttccgtgat gttaaagagg tgactggtcc gaactggcgc ggcggtgtgg cgactatcgg
    13261 cgtaacgcga gatggcaagc cggaaacggt gctgtatgcg gaaaaacgtt attacaacac
    13321 tgccgggtcg atgatgaccg aagcggcgat tgacggcggc atcacccgtg acctgtacgc
    13381 ggcactcggt gaagaactgg aaaacggtgc ctgggctgtt cgtctttact acaaaccgtt
    13441 tgttcgctgg atctgggcgg gc
//
LOCUS       DAZGBC010000134        13451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGBC010000134 DAZGBC010000000
VERSION     DAZGBC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.135"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..861
                     /locus_tag="ACHK1J_003776"
     CDS             31..861
                     /locus_tag="ACHK1J_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO7912993.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESEGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGIANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1002..1775)
                     /gene="uxuR"
                     /locus_tag="ACHK1J_003777"
     CDS             complement(1002..1775)
                     /gene="uxuR"
                     /locus_tag="ACHK1J_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO7912994.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1990..3450)
                     /locus_tag="ACHK1J_003778"
     CDS             complement(1990..3450)
                     /locus_tag="ACHK1J_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO7912995.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3531..4715)
                     /gene="uxuA"
                     /locus_tag="ACHK1J_003779"
     CDS             complement(3531..4715)
                     /gene="uxuA"
                     /locus_tag="ACHK1J_003779"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO7912996.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5055..6398
                     /gene="gntP"
                     /locus_tag="ACHK1J_003780"
     CDS             5055..6398
                     /gene="gntP"
                     /locus_tag="ACHK1J_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO7912997.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLIGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6572..7474)
                     /gene="fimH"
                     /locus_tag="ACHK1J_003781"
     CDS             complement(6572..7474)
                     /gene="fimH"
                     /locus_tag="ACHK1J_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO7912998.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPNYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7494..7997)
                     /gene="fimG"
                     /locus_tag="ACHK1J_003782"
     CDS             complement(7494..7997)
                     /gene="fimG"
                     /locus_tag="ACHK1J_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO7912999.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8010..8540)
                     /gene="fimF"
                     /locus_tag="ACHK1J_003783"
     CDS             complement(8010..8540)
                     /gene="fimF"
                     /locus_tag="ACHK1J_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO7913000.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8550..11186)
                     /gene="fimD"
                     /locus_tag="ACHK1J_003784"
     CDS             complement(8550..11186)
                     /gene="fimD"
                     /locus_tag="ACHK1J_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO7913001.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVVCAFAAQTPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTSWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLSQLSAECR"
     gene            complement(11253..11978)
                     /gene="fimC"
                     /locus_tag="ACHK1J_003785"
     CDS             complement(11253..11978)
                     /gene="fimC"
                     /locus_tag="ACHK1J_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO7913002.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12015..12554)
                     /locus_tag="ACHK1J_003786"
     CDS             complement(12015..12554)
                     /locus_tag="ACHK1J_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913003.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12619..13173)
                     /gene="fimA"
                     /locus_tag="ACHK1J_003787"
     CDS             complement(12619..13173)
                     /gene="fimA"
                     /locus_tag="ACHK1J_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7913004.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         3267 a         3474 c         3239 g         3471 t
ORIGIN      
        1 tcagtgaaat actcataaaa ggtaactatt atgtctatgc cattaagcaa tacccttcag
       61 agccaaatta ttacagataa tcacttcctt catcatccaa aagtcgacag tgagttaaca
      121 cgtaaatatg aacgtgcacg cctggatacg gaaaatatct atcttctgcc tcttgctcgt
      181 ggcaataacc ataattacga tggaaaaagc gttgtcgaaa ttagaaaact tgatattagt
      241 aaagagtcct ggccatttaa ttatgtgaca gaagcatgca gagaatccga aggtattaca
      301 acaacaggac gtatgctcta tcgaaacttg aaaatcacct cagctttaga cgaaatatat
      361 ggtggcatat gtaaaaaggc gcatgctgct acagaattag cagaaggttt acggctaaat
      421 ttatttatga agagcccttt cgatcctgtt gaggactata cagttcatga aataacatta
      481 ggccctggtt gcaatgttcc aggttacgcc ggaaccacga taggatatat ttcaaccctt
      541 ccagtttctc aggcaaaaag atggaccaat gagcagccaa gaattgatat ctatattgat
      601 cagataataa ctgtctcagg gatagcaaat tcttccggat tcgcccttgc cgcattactt
      661 aatgctaata ttgagatggg aaatgaccca atcattggta tagaagccta tcctggtacc
      721 gcggagatcc atgcaaaaat gggatataac gttatccccg gtgatgaaga tgcgccactc
      781 aaacgcatga ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa
      841 tggaattata ttggtaaata gccactgaga agaatgagta ctaatattca ggttatacag
      901 atatttaaac aacaccctgc aatttacaaa gtaatactca ttgcgacttc ttaatttaag
      961 aaagcgcaag gaacgtttac ccttgcgctt attataataa gtcaggcgtc gactttatcc
     1021 agcggccatg aatcaaacag atagccgtca aaataaatat cgtcaacgtt cgagaattcc
     1081 agcagacgtt gcttaacgtt ttccagatgc tgccacattg ccagcttcgc cgctcgggca
     1141 tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac
     1201 aggctgtcat ccagatggct gtgcaactgg atccacattg gattgttttc ccgccactgc
     1261 caggactgac ggaataactc caccagcatg ctgttatgcg ttgcttcggc aatggcgaga
     1321 tggaactgca tatcaccgct ttcgctgcta cccggcgcgc tggaagccag ttcacgctct
     1381 tccagttgca atgcctgacg cattttgacg atatcttcgc gggtagcctg cagggcggca
     1441 aactcggcga tgttactttc cagtaactga cgcgcctgta acagctcaaa aggacccgca
     1501 tcattgcaga cgttggcatc cggactgtct gtgttgtggc tgcctgagct atcaagaaca
     1561 tagataccgg caccccggcg cacttccacc agccctttga tctccagcat gatcagcgct
     1621 tcacgtacca ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggcggtagc
     1681 cgttcaccag gattgtacgg cgtctttatg atcagatcgc ggatcatcgc cccgacttcc
     1741 tggtaaggtc tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt aggtgcggtt
     1801 aatcaaattt ttgccggatg cggcgtgaac gccttatccg gcctacaacg ggcaatttta
     1861 cggcccccgt taatctcaaa ctataccatt agcactggaa gttggcacgt acaggtgggc
     1921 aaaaaaacga aaactggtca accaaatatc agtttgattg accagtaagt ctgtagggta
     1981 atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca gctgctgata
     2041 tgccgccgtc accgcgccaa caaagtcggc attctgcggc agatcatcgg caaaaatacc
     2101 gctcaggccc agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt taatcttctg
     2161 gaactcggcc agcatcgggt cgaccacgtc aatggcatta ccctgctcat ccacgccctg
     2221 ggtgtaacgc atccagccag ccacgcccag cgccaggtga cgccagctgc cgccgttttg
     2281 caggtgcaga cgcaccggat ccagcagacg ctgcggtaac ttctggctgc cgtccatcgc
     2341 aatctgccag gtacggtgac gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc
     2401 ataggcgttc aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat
     2461 cagggcaaag gccgctttgc gataatccgg gttagtcatg gtgtcggcaa tggtttcata
     2521 gccgccgagg taaccgaggt acgccaggaa agagtggctg ccgttcagca tacgcagttt
     2581 catcatttcg aacggcacaa cgtctgcaac gaactgtgcg cccactttat cccagtccgg
     2641 acgaccatta acgaagttat cttcaatcac ccactgacgg aacggttcgc aggcaatggc
     2701 gcacgggtcg taaacaccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc
     2761 cggaacgatg cggtcaacca tggtgcacgg gaaggtgacg ttctcttcaa tccatgccgc
     2821 cagctgcggg tcacgcgcct gagccagccc cagaaccgcg accttcgcca catgaccgtt
     2881 ttcacgcacg ttgtcgcagg acatcaccgt aaacgctttc aaccctttct cacgacgcag
     2941 acgcaacgct tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt tttccagatc
     3001 gtgcttgatc agtgggttat tgagatccag ttgaccgctt gccgcatcag cgcagtagcc
     3061 tttttccgtg accgttagag agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat
     3121 accttcgcag ccgtcgattt ccggatgcag cgcttctttc atcgaaccga taattttcag
     3181 ctcggtgctt tctgcgcctt tttccgctac ggtgtacagc agttgctgtt ttttcaggtt
     3241 ttcgatcagc acgcggtcgt tgcctggcat caggttaact tcgcagatgc cccagtcgct
     3301 gtcggtgctt tccagcagat gatgggtata cagcgcctgg tgcgcgcggt gaaacgcccc
     3361 gcaaccgaga tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac
     3421 cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcagagagt
     3481 gacggcaagg aagaaggaac cggcacggcg cgcagccatg ccggtggata ttaacggcta
     3541 aagaaagcgc gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga
     3601 ccaattgcgg agtaacctgg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg
     3661 tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct
     3721 tcaacaatcg ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg
     3781 tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc
     3841 ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg
     3901 gtgcacatgg tgaaaccgtt cgccatgctg tttacggtat caaccatcca ctgcatatct
     3961 tcaatggtgg aaacaatgcg cggcaggccg aggatcgggc gcggcggatc gtccgggtga
     4021 accgccatac gcacgccaac ttcttcagca actggaataa tcgctttcag gaagacggca
     4081 aagttttcgc gcagcttcgc tttatcgata tctttgtaca gctccaggtg cttacggaac
     4141 tggtcgaggg tatacccttc ttccgcgccc ggcagaccgg caatgatgtt acgcgtcaga
     4201 cgcgctttgt cttcgtcgct catagtggcg aagcgttcag ctgcctgagc aatttcttct
     4261 tcggtgtaat ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat
     4321 tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc gaggtcagta
     4381 cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatacc gcactgcgcc
     4441 aggttgcgca gagtctgctg atagttagcg atccactgct catagttgcc agtgtgggtt
     4501 ttgatatctt cgtgaattgg tacgctttct acgacagacc acaccaggcc tgcgtcttca
     4561 acgatcgcct tgcgtttgag gatctcttct acggaccata cttcgccgtt cgggatatgg
     4621 tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctaa agaaaccgga
     4681 tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg
     4741 ttccacagca aagagacgcg gaaccctgga gttgtttagg tatccggatg aacaaccaga
     4801 ccgcagccgt ggcgttgcat cggatgagat ggcgtataag ttctaccttt tggtaagaca
     4861 tgtcaacccg aattctaaaa ttggttaacc acatcacaag aatttcactc tcatggttgt
     4921 tgctgcaaat tgcagcaaag ggtaattgat ggttattgat cagaggatgt gacattcatc
     4981 gcaacaatgg ttgaccaatt tacataacat atcgcccaaa ataacaacgg ttcaaccagg
     5041 taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt ggtattcggc attggtctga
     5101 tgctggtact gaatttgaag ttcaaaatca attcaatggt ggctttgttg gtggcggcgc
     5161 tgtccgtcgg gatgctggcg ggcatggatt tgatgtcgct gctgcacacc atgaaagcgg
     5221 gcttcggcaa cacgctgggg gaactggcta tcatcgtggt gttcggtgcg gtcatcggta
     5281 aattgatggt cgactccggc gcggctcacc agatagcgca tacgctgctg gcgcgtctcg
     5341 gtctgcgcta tgtacagctg tcagtgatta tcatcggcct gatttttggt ctggcgatgt
     5401 tctatgaagt ggcctttatc atgttagcgc cgctggttat tgttattgcc gccgaagcta
     5461 aaattccgtt cctgaaactg gcgatcccgg cagtagcagc tgccactacc gcacattcac
     5521 tgttcccacc gcagccgggt ccggtggcgc tggtgaatgc ttatggcgca gatatgggga
     5581 tggtttatat ctatggcgta ctggtgacga tcccaagtgt aatctgcgca ggtctgatcc
     5641 tgccgaagtt cctcggcaat cttgagcgcc caacgccatc attcctgaaa gcagatcaac
     5701 cggtagatat gaacaatctg ccctctttcg gcgtttcgat tctggtgccg ctgatcccgg
     5761 cgatcattat gatctctacc accatcgcca atatctggct ggtaaaagat acccctgcct
     5821 gggaagtggt taactttatc ggttcctcgc cgattgcaat gtttattgcg atggtggttg
     5881 cattcgtact ctttggcacc gcacgtggtc atgacatgca gtgggtgatg aacgcttttg
     5941 aaagtgcggt gaaaagcatt gcaatggtga ttctgatcat cggtgcgggt ggcgtgctga
     6001 agcaaactat catcgacacc ggcattggcg acaccatcgg catgttgatg tcccacggca
     6061 atatctcgcc ctacattatg gcatggctga tcactgtgct aattcgtctg gcgacgggtc
     6121 agggtgtcgt ttcggcgatg accgccgccg ggattatcag tgctgcaatc cttgatccag
     6181 caactggtca gctgattggc gtgaatccgg cgctgctggt gctggcgacg gctgcgggtt
     6241 ccaacaccct cacccacatt aatgatgcct cattctggct gttcaaaggt tactttgacc
     6301 tgtcggtaaa agacacgttg aaaacctggg gactgctgga gctggtcaac tccgtggttg
     6361 ggctgattat tgtgttgatt attagcatgg tagcgtaaaa gaacaccaga gcctgccaat
     6421 ggcaggctca gttcggattg tcggcaaagt gcgctttgtt tatgtcagat gcgacgctga
     6481 cgcgtcttat ctggcctaca aagggctaac gtgcaggttt ttagcttcag gtaatattgc
     6541 gtacctgcat tagcaatgcc ctgtgatttc tttattgata aacaaaagtc acgccaataa
     6601 tcgattgcac attccctgca gtcacctgcc ctccggtacg tgcgtaattt gccgttaatc
     6661 ccagacttac cgccgaagtt cctactgctc ctaacgatac cgtgttattc gctggaataa
     6721 tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgcgc tggtgaaaac gacgcggtat
     6781 tggtgaaaat cgagttgccc gcatctgcgg ttgtgccgga gaggtaatac cccaggtttt
     6841 ggcttttcgc acaataaacg gtaagaggaa ttggcactga accagggtag ttcggcagag
     6901 taacggtgac atcacgagca gaaacatcgc agccgccagt aggcaccacc acatcattat
     6961 tggcgtaaat attccacaca aactggaaat catcgctgtt atagttgttg gtctgtcgca
     7021 aaataagcac ggcaattaat gagccagctt taatcgccac cccgcccgca ctgctcacag
     7081 gcgtcaaata aagcgccacc ggccacggct tatccgttct cgaattataa acaacccgcg
     7141 gcgtttcgct ggtggtcgga aatggatagc tactgccact atattttacg gtcccggaaa
     7201 aattagataa cacgccgcca taagccgagc ctcgttgcag tgtgacatag tctgtaatgg
     7261 tttccggata atcgttatgg caaaagattt gcgtcgaaag atctacgacc aggttttgcc
     7321 ccacattcac ggcaggcgca aggtttacat aaacattagc gctgccacca ccaataggga
     7381 tagcggtacc attggcggtt ttacaggcga atgaccaggc atttaccgac cagcccatca
     7441 gcagtacagc aaacagggta ataactcgtt tcattacaat catctcttcg ggttcagctg
     7501 taggtatagg tgatgctaat cactgcctga atggttccct gagtggctcc gccatttacc
     7561 gttaatgctc tgacctgtaa cgggaagtgc gctgattgtg aggaatcatc cacctgaact
     7621 gttttggttg cgccagtatt caatgtgttg ccactgtcat cctgtagctc taactggatg
     7681 ttttgcgcgg tcccctggtt tttataatat ccggtactgt cggctgcccc gctgaagctg
     7741 gcagtgaccc tcgacgttcc caccggacaa ttagtcaact caagcgcaac atcatgccag
     7801 gccgatgccg ccccggcaga catcagactg aaagaataaa gatcgccgag atcaaccgtg
     7861 gcattggtgg tggaaactgt gcacggtttg gcgacgacct taccgttcac cgtgatggtg
     7921 acatcggctg cctgtatcgt cgcacttgcg agcgccaata ttgccgccaa taaataccca
     7981 cgtttgcgcc atttcatgag catctccagt tactgatatt caagagtgaa ggtagccgtg
     8041 gcattgatat gccccgcagt gacaggcacc tgtgtcgcca ttagccgggc gtaaaaattc
     8101 aacgtatttg gtttacccgg cgtcagggtc gtccacgaaa gcgcggacga tggagcatta
     8161 aggggtattt gattttgctg ctcattcaga agctgtattc ccagtcccga agccgctgac
     8221 accgtatttt caagtgcaag caggttggca ttgtggctat ctgcaacacc ggtaaaccca
     8281 acttttacgg cagaaacggc attaccacag ggtgacagca aaatacgaaa tggaacgaca
     8341 ggagtcgtcg cgccaatgtt gttaaattgc ttcgccgcgt tttccatcaa atcaacagta
     8401 aaattggttg attcagcggc cacactacag ccgttatctc tgacatagcc gcggatagta
     8461 atcgtgctat ccgcagccaa agcgtgactc accgccagcc acaaaaaagc gcacagaaga
     8521 taaaaaggtt tgtttctcat cacgccccct taacgacatt cagctgatag ctggcttaat
     8581 aactgctgct gactctctgg tggcagttga taattggcga cacagtgagc attttcctct
     8641 tctccccatt tcacctgaac ttttcccgct aaaggcattc cgctgaggta aacctgacca
     8701 ttatccgcaa caatgccgct actctggcta ctctctgatg tcaccatcgc cccaaacggc
     8761 agcggcttat tattgtgggt tagcgtcatg agcagtttta tcccaacgcg cgctttaaac
     8821 tctgctcgca cgatcgcccc acgagtggga acaacgttag cgaccgcgtt atctaaatcg
     8881 acgttatcag ccagggtatt ggtatccagc gccactctgt tttcccgata ttcggtggca
     8941 taaggcagca cggcataacc gcgccagtcg gtacgcaccc ccgtctggtt ttcgactttt
     9001 gcatcttttg cgccaggcgc tttaacaagc accaccgtat cgtttaacgg ctgccccagc
     9061 gttacgccat tggcatgagc cagtacccca ccgctgactc cgtaatagag ctgcttaata
     9121 tcatcgctat ggctgtaacc gatattggca ttgccgtaac caccgcgata attcagcgtg
     9181 gcgtagcctg tgcttccgct attaccatcg cctcccccgg catagccggt ttgcacgcta
     9241 tagctgaggt tgttgtcttc cagcaacgta ccgtatacac cagccagatt ggtcatccga
     9301 ccgttgagat cgtgtgacat gctgtagctg gcactggcat gtcgccactg agatttgctg
     9361 tcagaacgca gccagtggct gaaaggaata ttgacgttaa gcgctaacat ctgatcccgt
     9421 cctttttgcc aggcgttttt cgtcaggcta tagctgagcg tccagttgat atcttcgaac
     9481 gcagtattta atccagcctg gaattgctca tcgacattac tcgttcccca ataagtttga
     9541 tggctaccac tcaaatacag tgttgatgtg cgcccgagtt gctgagtaac ggtgagctgt
     9601 aatttcccgc gtttgttata agcgaggttg taatagtcgg tgaatttcgg cttaacctga
     9661 ataactccat cctgtgtttc gatgttgtag ccattcattc gactgtatgt tgtatcagcg
     9721 aaattaaaat atccgctggt cgaataacgg taacccacta actgaatatt cgtgcctgac
     9781 tcattgagcg atttgttata gagaaaacgc accgattgtc cgtcatgctg actgtcatcg
     9841 ggaagtgtgg aattagcctg cgtcatatcc acagacagag cgcccagtgc ccccatgttt
     9901 ttcccgatac cgaaattaaa agcacgataa cgatccgcca gttgcgttcc accatatatt
     9961 gtccagccag ccggaaggcc gtggagtaat gtactctgga aaaagcgggg tttttcctgc
    10021 tgcgcatttc cactacggta ttctcctgcc gtaatggaat aacgagtatg cccttcacgt
    10081 tgcaaaagcg ggactgacga atagggtacg gtaaaaatct gcgtgctgcc gtcagcctct
    10141 ttgatcgtta cctgcaagtc accactatta cctgcggcat agatatcgtt gatggtaaaa
    10201 ggccccggtg gcaccgtact attataaatg tcatacccat tttgtttaat agtgacctgt
    10261 gcagtaccac gagcaatacc gtggatcacc ggggcaaatc ctctttggct atcgggtaac
    10321 atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa taccatcgaa aatatcgccc
    10381 tgggtataac catcacccag cgtcagccgg gaacgtaacg gtattatgtc tcgctcaagc
    10441 caggtattga tatgctgcca tttatttttg ctacctgatg aactgtcgct actgttataa
    10501 ctccagctgg tattgtcgcg taaacgccac gcaccaatat ttaacccact ctgtaggttt
    10561 aaatatgcat aatggctgtt acccccaatc cgattctgta cactatttcc gctgaaatta
    10621 taattgagca atccggcatt aataccggga tcccataact caggaggaat ataaccacgc
    10681 gcgcgattac tcataaatgc ctgagggatc gtcaggttca gtcgctgctg accaacatct
    10741 aaatgcgcag tagcgtcctg gaccattgtg gttaatggca cgcaggcatc atccgccagc
    10801 agattcatac cggagacaga agccgtattc agccccatac tggcaagttg cgcgcgtgtc
    10861 aggcagggaa caatcccttg ttcgctgtcg cccgtattaa atgtgacatc acgcgttgcc
    10921 atataaccat tattcaaata gatatcgaca cgatacgtcc ctggcggtaa ttcttgccca
    10981 ttttcaaaac gcgataaatc agccacagcc tggggatcat ccgctaaaaa gcgcggatta
    11041 aaatagagtt cggcagatga caaaggtgtc tgtgcggcaa aagcacagac gacaacgagc
    11101 cggacaaaaa aaccagccaa acgatgctta cgaatatgca agcattgtgt gtttcgctgg
    11161 taaagtctta aattcagata tgacattatt cctccggttt aaaatcaccc ggcagtcaat
    11221 tctttttatt caggcgaaaa attccccctg cgttattcca ttacgcccgt catttttggg
    11281 gtaagtgcac cataatcatt tattgttcgg taagtaatat tgcttcctgc atcagaaggc
    11341 aatttaaccg tgctttcgcc cattggaggc accaatgcat tttcaagaac ccgggttccg
    11401 gcattcaact ctgttaccgt caggtaatag ggtgtcgggt taatcagcgt cagagaattc
    11461 gcgctacgac gaaatcttaa tttttctgcg gcctgatcgg gtggcaacgc taatttagcc
    11521 ggacgatagt acagtttaat gcggctgata attgcgagct gtagcgtatt ctcagtcaat
    11581 tttgatttat ccattgacgg aatcgcttta acgttcatcc agaataaact ttcccggtcc
    11641 tgtggcaatt ggttatttgt tgcatcaaga atacgcaagg tattctcttt ttttcccttc
    11701 atcgcaaaca gaggaggcgt cacgataaaa cgaccatcct ttacaccatc ggcattttcc
    11761 acccatgatt gaattaaata ggtactattt tcatcattat ttgtcacggc aagttgcact
    11821 tgtttttgcc ctgccggata aattacgcga gtcgcaccta aggccactcc cgcttcagcg
    11881 cgtccggcaa ccatcattgc catgaacatc aggatacctg ccagcaagca gaatgttatt
    11941 tcctgcgatt tccttacatt gacgttttta ttactcactg tcctgttcct gttatcacat
    12001 tatctgctga acaattactg ataggttaaa gagaaccagg cctgtgcatt ggcgatgccg
    12061 ccagtaaccc gacgcccggt agcacgatat ttggcgatga aatgtagcga agtagagcct
    12121 gagtaaagcc gtttccagtt tgctggagga cgattaatcg gtacgaggtt tccttcatca
    12181 tcaaacaacg ctacgccaat attggtggct atccctggcc cctctcccac ggaaagcaca
    12241 tccggatttt taccatccgc gacaccgtga aacgccacac ctacacgttc actcaccacc
    12301 gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg cgctatcttc ccctaccgca
    12361 tgaaaccggt tactgctgat ttgccccata ttgaccgtca tttgtttatc accggcttca
    12421 atccggcaag tttccgcaat aatgacgccc tgaaattgca tatttccgcc gggcagcgtg
    12481 gtattccatt tatttccggc cagagcaaac attggcagca acgacgctaa tagaaataat
    12541 ctcttccttt tcatcgttat ttttatcgca caagggtggg catccctgcc cgtaatgacg
    12601 tccctgaacc tgggtaggtt attgatactg aaccttgaag gtcgcatccg cattagcagc
    12661 acccggggtt gcctcgccga ttgcataata acgcgcctgg aacggaatgg tgttggtacc
    12721 gttattcaag gttgtttgtg cactaaatgt cgcaccgtcc agcgtcagct catttcctgt
    12781 tctgtccagt atctgcacac caacatttgt tgcgctaccc gcagctgaac tctgcagagc
    12841 cagtacttta ggatgagcac tgtcaatcgc agtccccaag aaggcaacag cggcttttgt
    12901 ggcaacactg gtatcgcaat cattcagctg aatattaaaa ccgacagcag agctggttgc
    12961 tccagtctgc ttcagactag cggtacgaac ctggcccaac tgaacggttt gatcaacaga
    13021 gcctgcatca actgcgcaag cggcgttaac aacttcccct ttaaagtgaa cggtcccacc
    13081 atttaccgtc gtgggtgtag tttcagccag agccgctgcg gaactgaggg acagagccga
    13141 cagaacaacg attgccagag ttttaatttt catgctgctt tcctttcaaa aaactatttc
    13201 taaatcgaca tgggcagtcg ttctgtacac tttgttttgt caacgagttt gactgccaac
    13261 actgcacagt tttcccccaa aagatgaaac atttggggcc attttgactc atagaggaaa
    13321 gcattgcgca caaacttttt cagttaattt gttggcttaa tattctattg ttatctttat
    13381 ttatagattt ttatattgca tgaggtggtt tttggagaga agaatgagga agaggcgtcg
    13441 agccacagaa a
//
LOCUS       DAZGBC010000135        13414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGBC010000135 DAZGBC010000000
VERSION     DAZGBC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.136"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2208
                     /gene="crfC"
                     /locus_tag="ACHK1J_003788"
     CDS             <1..2208
                     /gene="crfC"
                     /locus_tag="ACHK1J_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO7913005.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQRALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLI
                     RHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     VAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQ
                     VEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQDDSEPLSE
                     IRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPG
                     FHFPVFHGVVPHFNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
     gene            2205..3083
                     /gene="rdcB"
                     /locus_tag="ACHK1J_003789"
     CDS             2205..3083
                     /gene="rdcB"
                     /locus_tag="ACHK1J_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO7913006.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            3347..4849
                     /gene="proP"
                     /locus_tag="ACHK1J_003790"
     CDS             3347..4849
                     /gene="proP"
                     /locus_tag="ACHK1J_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO7913007.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4961..5050
                     /gene="pmrR"
                     /locus_tag="ACHK1J_003791"
     CDS             4961..5050
                     /gene="pmrR"
                     /locus_tag="ACHK1J_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO7913008.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5026..6126)
                     /gene="pmrB"
                     /locus_tag="ACHK1J_003792"
     CDS             complement(5026..6126)
                     /gene="pmrB"
                     /locus_tag="ACHK1J_003792"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO7913009.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6127..6794)
                     /gene="basR"
                     /locus_tag="ACHK1J_003793"
                     /pseudo
     CDS             complement(6127..6794)
                     /gene="basR"
                     /locus_tag="ACHK1J_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
     gene            complement(6791..8434)
                     /gene="eptA"
                     /locus_tag="ACHK1J_003794"
     CDS             complement(6791..8434)
                     /gene="eptA"
                     /locus_tag="ACHK1J_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO7913010.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8538..9875)
                     /gene="adiC"
                     /locus_tag="ACHK1J_003795"
     CDS             complement(8538..9875)
                     /gene="adiC"
                     /locus_tag="ACHK1J_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO7913011.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10012..10773)
                     /gene="adiY"
                     /locus_tag="ACHK1J_003796"
     CDS             complement(10012..10773)
                     /gene="adiY"
                     /locus_tag="ACHK1J_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO7913012.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11098..13365)
                     /gene="adiA"
                     /locus_tag="ACHK1J_003797"
     CDS             complement(11098..13365)
                     /gene="adiA"
                     /locus_tag="ACHK1J_003797"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO7913013.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3218 a         3294 c         3530 g         3372 t
ORIGIN      
        1 gagttaagtc aggaggctga acgcctgtta cagctttctc gccaacagtt gcagttactg
       61 gaaaaaatgc ctctctctgt acccggagac gatgcgccac aacgggcttt accctggagt
      121 cagcctaata tcgccgagcg tcacgcgatg ctgaataatg agttgcgtaa aatttcccga
      181 ctggaaatgg tgctggcgat tgtcggtact atgaaagcag ggaaatcaac caccattaat
      241 gccattgttg gtacggaggt actgcctaat cgcaatcgcc caatgactgc gttgccgacg
      301 cttattcgcc atacgcccgg gcaaaaggaa ccggtactgc atttttcaca tgtcgcgcca
      361 atcgattgtt taattcaaca attacaacag cgcctgcgtg attgcgatat taagcatctg
      421 accgatgtgc tggaaataga taaagatatg cgtgcgctta tgcagcggat cgaaaatggc
      481 gtcgctttcg aaaaatatta tctgggtgcc cagcctattt ttcattgtct gaaaagtttg
      541 aatgatttag tgcgactggc gaaggcgctg gacgtcgatt ttcctttttc tgcttacgcc
      601 gccattgagc atattcccgt gattgaagtg gagtttgtcc atctggcggg gctggagagt
      661 tatcccggtc aattgacgtt actggatacc cccgggccaa atgaagccgg gcaaccgcat
      721 ctgcaaaaaa tgcttaacca gcagctggca cgcgcctcgg cggtactggc ggtgctggat
      781 tatacgcaac tgaaatcgat ctccgatgaa gaggtccgtg aggcgatttt ggcggtgggg
      841 caatcggtgc cgctgtatgt gctggtcaat aagttcgatc aacaggatcg taacagtgac
      901 gacgccgacc aggtgcgggc actgatttcc gggacgctga tgaaaggctg tattacgcca
      961 cagcagatat ttccggtgtc gtcgatgtgg ggctacctgg cgaatcgggc gcgccatgag
     1021 ttagccaaca acggtaagtt accagcgcca gagcaacaac gctgggtgga agattttgcc
     1081 catgccgcgc tcggcaggcg ctggcgtcat gccgatctgg cggaccttga acatattcgt
     1141 catgctgccg atcagttgtg ggaagattcg ctgttcgccc agccaattca gatgttgctt
     1201 catgccgctt acgctaacgc ctcgttgtat gctctgcgat ctgccgcgca taaactgttg
     1261 aattacgcgc agcaggcgcg ggaatacctg gattttcgtg cgcacgggtt aaacgtcgct
     1321 tgtgaacaat tgcggcaaaa tatccatcag gtcgaagaaa gtttgcagct attgcaactc
     1381 aatcaggcgc aggtgagcgg cgagattaaa catgaaatcg agctggccct gacctccgcc
     1441 aaccactttc tgcgtcaaca gcaagatgcg ctgaatgcgc agttagccgc cttgtttcag
     1501 gatgattcgg agccgttaag cgagattcgt acctgctgtg agacactgtt acagacggcg
     1561 cagaacacca tcagtcgcga ctttacgctg cgttttgccg agcttgaatc caccctttgc
     1621 cgggtgttaa ccgatgttat tcggcccatt gagcaacaag tcaaaatgga attgagcgag
     1681 tcagggtttc gtcctgggtt tcattttcct gtttttcacg gcgtagttcc ccacttcaac
     1741 actcgccagc tgttcagtga agtcatttcg cgccaggatg caacggacga gcagagcacg
     1801 cgtttaggcg ttgtgcgtga gactttttcg cgctggttga atcagcccga ctggggacgg
     1861 ggaaatgaga aatccccgac agaaacggtt gattacagtg tgttgcaacg agctttaagc
     1921 gcagaagtcg atctttattg ccaacaaatg gctaaagttc tggcagagca ggtcgatgaa
     1981 tctgttacgg caggcatgaa tacttttttc gctgagttcg cttcatgttt gacggaatta
     2041 cagacgcgtt tacgcgaaag tctggctctg cgtcaacaaa atgaatcggt ggtcaggctg
     2101 atgcagcagc aattgcagca gactgtgatg actcacggct ggatttacac cgacgcccag
     2161 ctgttacgcg atgatattca aacacttttc acggcagaac gatattgacc aagacgttac
     2221 ttgacggccc cggtcgcgtg ctggagtcgg tttatccccg ctttttagtg gatctggcgc
     2281 agggcgatga tgcccgcctt ccacaagccc atcagcagca gtttcgtgaa cgactgatgc
     2341 aggaacttct tgcgcgcgtg cagcttcaga catggacgaa cggcggcatg ttaaatgcgc
     2401 cgcttagcct gcgactgaca atggtggaaa aactggcgtc gatgctggat cccggtcatc
     2461 tggcgctgac gcagatcgcg cagcatctgg cgctgctgca aaaaatggat caccgccagc
     2521 actctgcttt cccggagctc ccccagcaaa ttgccgcctt gtatgagtgg ttttcagccc
     2581 gttgccgctg gaaggaaaag gcgttaacgc aacgaggcct actggtgcag gcaggtgatc
     2641 agagcgagca aatttttacc cgctggcgtg ctggggcgta taacgcctgg tcgttgcctg
     2701 ggcgctgttt tatcgttctc gaagagttgc gttggggggc atttggcgat gcctgtcgtc
     2761 tgggaagccc gcaagcggtg gcgttgttgc tgggtgattt gcgcgtgaaa gcgacacaac
     2821 atctggcaga gagtatcaat gcggccccga ccacgcgtca ctattaccat cagtggtttg
     2881 cctcttcgac cgttccgacg ggcggggatc atgctgattt tttaagttgg ctgggaaagt
     2941 ggaccacggc agataaacaa cccgtttgct ggtcagtgac ccaacgctgg caaactgtcg
     3001 cgctggggat gccacgactc tgttcagcgc agcgtctggc gggggcaatg gtcgaggaaa
     3061 tcttctctgt aaatttggcg taaataatca gttacatcaa tgagtcctaa acgaaatcca
     3121 tgtgtgaagt tgatcacaaa tttaaacact ggtagggtaa aaaggtcatt aactgcccaa
     3181 ttcaggcgtc aactggtttg attgtacatt ccttaaccgg agggtgtaag caaacccgct
     3241 acgcttgtta cagagattgc atcctgcaat tcccgctccc cttttgcggc cgtcgcgctg
     3301 atttttctgg cgtttgcgga aatgggccaa ctctgcgagg aaagctatgc tgaaaaggaa
     3361 aaaagtaaaa ccgattaccc ttcgtgatgt caccattatt gatgacggta aactgcgtaa
     3421 agccattacc gcagcatcac tgggtaatgc aatggaatgg tttgattttg gtgtttatgg
     3481 ttttgttgct tacgcattag gtaaagtttt tttcccgggg gctgacccca gcgtgcagat
     3541 ggttgctgca cttgccacgt tctccgttcc ctttctgatt cgaccgcttg gcggactctt
     3601 ctttggtatg ttgggcgata aatatggtcg ccagaagatc ctcgctatca ctattgtgat
     3661 tatgtcgatc agtacgttct gtattggctt aataccgtcc tacgacacga ttggtatttg
     3721 ggcaccgatt ctgctgttga tctgtaagat ggcacaaggt ttctcggtcg gcggtgaata
     3781 taccggggcg tcgatatttg ttgcggaata ctcccctgac cgtaaacgtg gctttatggg
     3841 cagctggctg gactttggtt ctattgccgg gtttgtgctg ggtgctggcg tagtggtgtt
     3901 aatttcgacc attgtcggcg aagcgaactt cctcgactgg ggctggcgta ttccgttctt
     3961 tattgctctg ccgttaggga ttatcgggct ttacctgcgc catgcgctgg aagagacccc
     4021 agcgttccag cagcatgtcg ataaactgga acagggcgac cgcgaaggtt tgcaggatgg
     4081 cccgaaagtc tcgtttaaag agattgccac caaacactgg cgcagcctgt tgacatgtat
     4141 tggtctggta attgccacca acgtgactta ctacatgttg ctgacctata tgccgagtta
     4201 tttgtcgcat aacctgcatt actccgaaga ccacggggtg ctgattatta tcgccattat
     4261 gatcggtatg ctgtttgtcc agccggtgat gggcttgctg agtgaccgtt ttggccgtcg
     4321 tccgtttgtg ctacttggta gtgttgccct gtttgtgttg gcgatcccgg cgtttattct
     4381 gattaacagt aacgtcatcg gcctgatttt tgccgggtta ctgatgctgg cggtgatcct
     4441 taactgcttt acgggcgtta tggcttctac cttgccagcg atgttcccga cgcatatccg
     4501 ttacagcgcg ctggcggcgg catttaatat ttcggtgctg gttgccggtc tgacgccaac
     4561 actggcggcc tggctggtcg aaagctcgca gaatctgatg atgcctgcct attacctgat
     4621 ggtagtggcg gtgattggtt taatcaccgg cgtaaccatg aaagagacgg caaatcgtcc
     4681 gttgaaaggt gcgacaccgg cggcgtcaga tatacaggaa gcgaaggaaa ttctcgtcga
     4741 gcattacgat aatatcgagc agaaaatcga taatattgac cacgagattg ccgatttgca
     4801 ggcgaaacgt acccgcctgg tgcagcaaca tccgcgaatt gatgaataag ctgaaacgga
     4861 tggcctgatg tgacgctgtc ttatcaggcc aattgaactc ttaaggttca cttaatctct
     4921 gacgcgcata ctctcctcca ggttaacgga ggagagtgca atgaaaaacc gtgtttatga
     4981 aagtttaact accgtgttca gcgtgctggt ggtcagcagc tttctttata tctggtttgc
     5041 cacgttctga tctttcttca gccgtaccca ggcccgcgtg ccggaagtct cttgccggtt
     5101 ttgcaggaaa aactgcccgt gatgcaactg tgtaatgcgg ctgacaatac ttaaccccag
     5161 accaatcccg ccataacggc tgtccatacg tacaaacgct ttactcaact ccccgcattt
     5221 actctcatca atacctggtc cttcatcttc aactgccatg accgctccgc catcttcttg
     5281 cagcttaatc ataatgttgc tgccttgcgg gctgtaacga tgggcgtttt ctaccaggtt
     5341 tcgcaataac atccgcagca gggttgcatc accctgaacg gtgatgtcgg cggcgctctc
     5401 tggcaatagc agggtttgct gtcgctggtc gagcatggta ctgagttcgt catacgaggg
     5461 gagaatgaca tcttccagca gttttacatg ttgataatta ccggaagaaa atgactgtcc
     5521 ggcacgcgcc agttgcagca gctgggagac gctctccatc atctgatcaa gccgtgccac
     5581 taacggtgct acatcaatgt gatgcgtttt cgccagcagt tccagatgca aacgcacccc
     5641 agccagtggc gttcgcagtt cgtgcgcgac gtcagcggta aacaaccttt cgttatccag
     5701 cgtgctggtc aggcgactga ccagatcgtt caacgccgaa accaccgctt cgatttcgag
     5761 ggtggcgctg tgaatggcaa tgggcgttaa gttgtcggcg gtgcgcgctt ccagctcttt
     5821 ttgcagctcc gccagcgggc gggtgatgcg gcgtaccgcc tgatagcaga taaatagcgt
     5881 caggctgacc ataaacacgc cggggacaat caggctggcg accgcctcgc ggatctcacg
     5941 catgatgtgg cgatcgttgt tgcgattgtc gcgtagcgcc tgctcaaaca gctgaatctg
     6001 ctcggtactt tcatgccata gccagaagac gctgatcagc tcaaacacca acaaaatggc
     6061 cccgatggtc aatatcagcc gttggcgcag cgatattggt cggcgcagaa aatgcatcag
     6121 attcaattag ttttcctcat tcgcgaccag catatagcca aagccgcgca cggtgcggat
     6181 acgggctttg cccactttgt cgcgcagatt gtggatatgc acttccaggg tgttggtcga
     6241 gggttcattg tcccagttat agatgtcgtt gtagagaatt cccgatgcac cggactgcct
     6301 gctttgagca ttaaccgtga cagcagagca tattctttgg gcgtcagaat caactcttca
     6361 ccgcccatcc atacctgacg gcgacccata ttcagcgtca gattgccaac aatcagctca
     6421 ctttcgccct gattattatg gcgtcgtagc agggcgcgga tacgggcgtg taactcttcc
     6481 agcgcaaaag gcttcaccag atagtcatcg gcaccgacat ccagcccggc gattttgtcg
     6541 gtcagcgtat cgcgagcggt gaggatcagt actggcaggg tgtatttttt ctgccggata
     6601 cgggcgagaa aatgcagtcc atcttcgtcg ggtaacccta aatccagtac caccaggctg
     6661 taatgaccgg cctcaaggct ttgttccgcc atccgcgcgg ttgtcacgcc atcgcacgcg
     6721 tagccttcgg tttgcgccgc cagaatcaat ccctgcaata acagcgtatc gtcttcaaca
     6781 atcagaattt tcattcactc actctcctgc aagtttgcag aatatcatcc gcagcctggt
     6841 aatacttcgt ctcaacgcca gttaatccca atagcgtgga gaataaattg tcttgtgaat
     6901 agtgttgcgt ttgcgcctgt ttttgcaggc agttctggtc aacctgatac cgtttttgat
     6961 aatcctccga cagccacagc agcattggca cctgtttttg gctatccggg gcgatggcat
     7021 aaggcagacc gtgcagatag atgccatttt cacctaacga ttcaccgtgg tcagaaagat
     7081 aaaccaggct ggtggtaaat ttatcctgat gttctttcag cagattaatc gctttatcaa
     7141 caatatagtc gacgtaaacc agcgtgttgt cgtaagtgtt caccagttgc tctttgctac
     7201 aggtctggat ctcattggtg tcgcaggttg gggtaaattt cctgaactgc ggcggatagc
     7261 ggttgtaata ggtcggaccg tggctgccga tggtgtgtaa gacaatcacg ccatcaccct
     7321 gcaggttatt gatgtactct tccagcccgt ggaacagcac ttcgtcatag cattcgccgt
     7381 tgatgcactg accaggcaga ttcagcgcgg tgacgttctg gtgaggcacg cggtcgcagg
     7441 cacctttaca gccgccatcg ttgtcattcc acagcacgtt gatgcccgct cgctgaatga
     7501 tatccagcac gccttcctgg tgctgtgcca gctcttcttt gtaatgctca cgcggcatat
     7561 ccgagaacat gcacggtact gaaactgccg ttgccgtgcc gcaagatgcg gtattaggga
     7621 aatagaccac gttatctttc gccagccgcg ggttagtttc acgcgggtag ccgttgaggg
     7681 agaagttctc cgcccgcgag gtttcgccga caatcaggat ggtcaaattt ttacgttttt
     7741 cgttctgcat taacgggttg cggtgggcgt cttcaccaat tcgcaccagc ggtagatttg
     7801 ccagtcgctg atgggagtac catgaccagc tggcaacaat gctgttagag gggcttaagg
     7861 atttcaccag ctctttgtta ttgcggaaca acgaggcgta gtctttataa aacagtgcgg
     7921 cgaccagcaa aatcagtagt acagaaacca gaatattggc tccacggaaa agaacactgc
     7981 gcagacgcga ggtggcaggt ttgattttta tccagcaggc aatcagcgca gcaagcacgc
     8041 cgctgaatcc cagcgttaat aacatttgcg gtgtcatcag cgcataactt tctgccggag
     8101 tggtatcaat aatattggca atcatcgagc ggtcgatgac gatgccgtaa gtcattatga
     8161 aatattgtgc agccgcgcca accagaataa acaggcaggc cagtggtcga ttaagccata
     8221 agaaagagct tagtgtcagg acaatattaa tcacgctgaa agcgacgacc ggcatcgaca
     8281 agaaaaccag tacgttatgc agcgaatcca gcggcagcgc ctgcaacacc tgtttaaaaa
     8341 aggcaatatt caggcagata gagatataaa aagcggccaa caatagccag gcgagtaaat
     8401 tcaaagaggg tctttttagt aggcgcttca acatcacggt gtttccatcg aacaaagtgc
     8461 gcatatgctc gcaaagcaaa attaagccaa ccttaagttc ttaaggttgg cttttatgtt
     8521 tgctggatta aggggaatta atctttgctt attggtgcat ctaagggata cgggttttta
     8581 tgtagccggt tgtaattcag cgcatacatg gcggtgatga ccatcagggt gacaaatgac
     8641 cacataacct ctttcgctcc ggaccccacc acggcccaga tgcagtagag gaaggcaatg
     8701 gtagtaactg ccagatatgc cgggcgtgct ttaccaaagt gaccgtgtcc gagcagcagt
     8761 aacgccgcac aggtgtaaag atatggcacc agtgtaaaga tgaccgagac ggaagaaacc
     8821 agaccgaact ctttggtcgc gtttggtgaa atgctgctga gctggaagat ggtcatcaaa
     8881 ataccgacga taatcagccc cgccactggc gtacccgctt tatttacacg ggcaaaaatt
     8941 ggtgggaaca gtccgtcatc ggcagcggct ttcgccgttt gacccgccag caacgtccag
     9001 ccgcccagtg aacctaagca acccgcagct gcgcagaagg aaacaatggc cccggcggtg
     9061 tcacccaacg ccatccgtgc ggcatcaccg aatggcgaag cagaaacgcg cagtgcggca
     9121 ttagggatca tccccataat cgcggtggta gaaagtacat agcaaacggc ggcaatcaat
     9181 accccaccaa tggtggcgat agggacattg cgtttcgggt ttttcaccac acctgcggca
     9241 acggaggcac tttccacacc gatgaacgac cacagcgtaa cgttaagggt actttgaatt
     9301 gcaccgaagg tgcccaggcc gctgacgttc catgccgcca tataggtttc accacggaac
     9361 cagaaccagc caaatacggc aatcccgacg atggggatca gcgccagcac ggtggcaact
     9421 gcctgcacac gggtgatcat tttcggaccg acaatgttca gcaggacgaa gatccacagc
     9481 accacgacgc aggtgatggt taataccaat ggatctttca gaatcgggaa gaagtaactt
     9541 aaatatccta cgccaatgac caccatggcg atattgccga tccagcaggc cagccagtag
     9601 aggacgttgg tttgataacc gagaaacggg ccaaagcagc ggcgggcgta agcgtaagaa
     9661 ccaccaggac ttgggtcgag gaacgacatt ttggcgtata ccatcgagag ccccagtgca
     9721 ccgataatcg tcaccaacca tccataaatg gcaatcccgc cagtagaggc caggtttgca
     9781 ggtaacagaa aaacacctga ccccataata ttccccgaca ccatcagggt gacggggatt
     9841 aagcccactt tgtgagcatc agcatccgaa gacataatta aactcctgcg aaggcgagct
     9901 tcgtgacaat aaattacgtc atatcatacg cctgcattcg atagttgaat tattatcgac
     9961 cgggttatcg ctgatgccgt tattaatata gctcttcgtt aaggttaaaa atcaggcgac
    10021 agtgcgttct ctgtgctgac taacataatg cagcggcgtc ataccgtaga agtctttaaa
    10081 gacagaaata aagtacgacg tactgttgta gccgcaggac tgtgatacct gagagatatt
    10141 tttaccgtcc atcattaatt catttacggc ataacgcatg cggcaggtgg tgattatttg
    10201 gctataactg gtgttttcgc ttttcaactt tttctttaac agacttgggc taagacataa
    10261 acaactggct accatactaa gattccagtc tttgtgaata tcgctttcaa taatttgata
    10321 aacgctgtca cttacacaat tacgtaacat atacatcatt aatgaaagga attttttact
    10381 gtcgagaaaa cgggatagca ccgtgaataa taatgcgcgt gttcgttcct tttctgactc
    10441 agtttctgcg ggcatcatgc tgtgttgcgc ggcaacgcga aagacgtctg gcgtcaggca
    10501 tggcagggtg agtatgggcg tagcgctacg ttgccatact ggtatttgcg agagatcttt
    10561 attcagaaac cggaggtaat ctttgatgat gtcgtgacta atatgagcaa ccaaagtgtt
    10621 gttaagagag gagacgtcga taatattatt ttcacaattt aataacgcca tatggttagc
    10681 tttaaggcta ataggttctt tcccattcac ccttatccag acatcttttt cagtcaataa
    10741 aacaatacaa ggttggtcgc tgcaaatcct catacatgta ctcctgagtg caaataaaag
    10801 accagttctc gctaaagcaa agcgataccg ggaaatggtt atataattgt atgatgaata
    10861 taaacatgca taaaaataac gagttttact aatattacaa atttttatag taatttctca
    10921 tgtacatatt tttttacagg ataaataaca ggcaaaaaaa agcgcggggt ggggccgcgc
    10981 aaaaagtatg acatttttgt ttgcagcaat ttttatgaaa acgttgccat actcaaaagg
    11041 cctaaggcca ggcggcgtaa tgttatttaa acaattacgc cttcagcgga atagtggtta
    11101 cgctttcacg cacataacgt ggtaaatacc gtcaataatt tcagtccctt cagtttcgtg
    11161 ttcaaatcca gggaaatggt ggtcccagga ttgcagcgag cgtaaataac ttacttgcgg
    11221 actgttttta tcgccgaagt tttcaccaga cagcagcatc gggattcctg gcggatacgg
    11281 gataactgag tttgccgcga tgcgtcctgg cagattttca atggatacca gttcgacatt
    11341 gttgtcgaca atcgcgttgt acgcttcacg cggggtgatt tccgccatcg gcaggccgga
    11401 ataggcttcg ttcaaccgtg cgccagggtt gttttctttc agccaggcaa acatggtgtc
    11461 acccagatcg tgaatcccca tgttcgcgta agtgtcagga tattgttcaa caagttccgg
    11521 catcacctgc gccagcggtg tgttggcgtc atagtggcgt ttgaaggagc aaagggtgtt
    11581 aaccagagtc ccccatttcc cacgggttac gcccatagag aacaggaaca taatttggaa
    11641 gtcagtggtg cgggtaggca caatgccgtg gcgaccaagc caggcagtga ccagcgccgc
    11701 cggaacaccg gtttcttcca gttcaccatc ttcacccatt cccggagcaa ggatgctgac
    11761 tttaattggg tcgagcatac tccagttatc cggaatatct ttgaagccgt gccagctttc
    11821 gcccggatgc attacccagc agtcctgaac ggtggtcagc agtttggttg gtgcgtcagc
    11881 aaagtcatag gttttgccgg tttgtgggtc ggtgacgact tctttgttcc acggtttgaa
    11941 gaaccagcta ccgtcagcgg tgaactcttt atatagccgc gccatcgcct gacggaaatc
    12001 aaccgcttcg tcaatcactt cctgtgtcag tgacaggccg ctgttgccgt ccatcatcga
    12061 caccgccacg tcgttggatg cgcagatggc atacagcggg gaggtggtgg catgcatcat
    12121 gtaggcctgg ttgaagcggg agaagttaat cgccccacga ccttcacgta catgaatata
    12181 agaagcctgt gacagcgcat tcagcagttt gtgggtggag tgggtggcga aaacggtagg
    12241 accgttgtga tcgcctggtt cgccgcgcat ggcatagtga tcggcataga tcgggttgaa
    12301 acgtgcatag ccgtaccagg cttcgtcaaa gtgcagacga tcggaggttt tttccagcag
    12361 atcctgcgct tctttagcgt tataacacac gccgtcatag gtgcagttgg tcaccacgca
    12421 gtaagacggt ttttgcccgg ctttgtcttt ggtcagcggg ctttcactga ttttcttctg
    12481 caaggtttca ggttgcattt cctgcggata gattggcccg ataatgccgt agcggttgcg
    12541 gcttggcacc atatagaccg gtttcgcgcc tgtcagcatc aaaccttgtt cgatggattt
    12601 atggcagtta cggtcaacga ccacgacatc gttatcggtc atgcaagcct gcatgatggt
    12661 gcggttagag ccggaagtac cgacgactac cgaccaggag cgatcggcac caaatacgcg
    12721 tgcggcatat ttttcgcttt cgccaaatgc gccagtatgg tcaagcaaag aaccgaggga
    12781 agttcgttcg atgcccatgt cggtgcggaa cagattttca ccatagtagt catggtagaa
    12841 acgtccggcg ggtgttttgg taaaaccaac gccgccctgg tggcctggcg ctgcccagga
    12901 atattcatgg atgtcactat atttcatcag cgcgctgaac agtggcggca acagctgctg
    12961 gcggtagcgg gtcatcgcgg caacggcgcg tccggcgata aagtcggcgg tatcttccag
    13021 aatccaggcg aattcatcga caagctccag cagatcccgg tccattgcgg cgagggcttt
    13081 ttcccgatcg cccaacagga agaccggcac gttttgttgg cgctcatgaa gcttaccgat
    13141 caattgtctg acgttttgat gttcgtccgg atgttccatt tgatagctga acatcaggca
    13201 gtcaatggct tcgtttgaag agagaatggc aaaaccatca tcaaaggagg tggatttaat
    13261 cacggtaaca ttttgctggc ttaaagcatc tgccagacgc tcaacggcgt taccgaccca
    13321 ggtgtcttga tggagaaact cgctttcaac aattaatact ttcatcattg cttacccggt
    13381 tatgaaggaa tcttcgttgc gggcaagtat cttc
//
LOCUS       DAZGBC010000136        13377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGBC010000136 DAZGBC010000000
VERSION     DAZGBC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.137"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(117..1271)
                     /gene="ompC"
                     /locus_tag="ACHK1J_003798"
     CDS             complement(117..1271)
                     /gene="ompC"
                     /locus_tag="ACHK1J_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7913014.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
     gene            complement(1470..1811)
                     /locus_tag="ACHK1J_003799"
     CDS             complement(1470..1811)
                     /locus_tag="ACHK1J_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7913015.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            complement(1804..5046)
                     /locus_tag="ACHK1J_003800"
     CDS             complement(1804..5046)
                     /locus_tag="ACHK1J_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7913016.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLSNP
                     GWTTGNGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQGALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(5110..5523)
                     /locus_tag="ACHK1J_003801"
     CDS             complement(5110..5523)
                     /locus_tag="ACHK1J_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913017.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(5587..5796)
                     /locus_tag="ACHK1J_003802"
     CDS             complement(5587..5796)
                     /locus_tag="ACHK1J_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO7913018.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            complement(5892..6266)
                     /locus_tag="ACHK1J_003803"
     CDS             complement(5892..6266)
                     /locus_tag="ACHK1J_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO7913019.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(6281..6997)
                     /locus_tag="ACHK1J_003804"
     CDS             complement(6281..6997)
                     /locus_tag="ACHK1J_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO7913020.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(7063..7407)
                     /locus_tag="ACHK1J_003805"
     CDS             complement(7063..7407)
                     /locus_tag="ACHK1J_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO7913021.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(7404..7850)
                     /locus_tag="ACHK1J_003806"
     CDS             complement(7404..7850)
                     /locus_tag="ACHK1J_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO7913022.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGIESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(7847..8197)
                     /locus_tag="ACHK1J_003807"
     CDS             complement(7847..8197)
                     /locus_tag="ACHK1J_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7913023.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(8207..8533)
                     /locus_tag="ACHK1J_003808"
     CDS             complement(8207..8533)
                     /locus_tag="ACHK1J_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO7913024.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(8530..10952)
                     /locus_tag="ACHK1J_003809"
                     /pseudo
     CDS             complement(8530..10952)
                     /locus_tag="ACHK1J_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(10898..11119)
                     /locus_tag="ACHK1J_003810"
     CDS             complement(10898..11119)
                     /locus_tag="ACHK1J_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913025.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVQRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(11164..13101)
                     /locus_tag="ACHK1J_003811"
     CDS             complement(11164..13101)
                     /locus_tag="ACHK1J_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7913026.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(13165..>13377)
                     /locus_tag="ACHK1J_003812"
     CDS             complement(13165..>13377)
                     /locus_tag="ACHK1J_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7913027.1"
                     /translation="AEGVLVHGGQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPL
                     MALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT         2924 a         4248 c         3114 g         3091 t
ORIGIN      
        1 tcagcacatg ccagatggca attgctgatc gcagtgacca atgaaaactg tgttttcatt
       61 gatacaggcc gcctgtaagg gcagacggcc cgtatttatt aacttatcag caataatcag
      121 aactgataag tcatacccac agcaacgata ttatctgtcg caacaccggc tttccgggta
      181 aattcacttt catcaatcag gttaatttta taatcaacat aggtggacat attcttgtta
      241 aagtaataag tcgcaccgac atcaatatat ttaaccagat cctgatcgcc atggttcaca
      301 ccaccaacca taacatcctg tccgcgggac tgaaggtagg caagggacgg gcgcagacca
      361 aaatcaaact gatactgaac aacggcttcg atattctgag ctttatccgc aacaccttga
      421 tcaccaaagg tcgtcatatt ctgggtttca gaatacactg ctgccagata caggttattg
      481 gcatcatatt taagaccagt tccccagaat tcagcatgtt taccttttgc cacactacct
      541 gagtcaactg ttttttctcc aaccttttta cctgattcag gatcaagaac atctgcagta
      601 ccgttcaacc cctgaacctg cttatcggta cgatctgact tggtatacgc agcgacaaaa
      661 ccaaaaccat caaactcata gctggccgag aaaccatacc cgtcgccatt agccttctga
      721 aggtcatcac gctcattttt gccctgatac tgcgccgcaa aattcagacc atcaaccagt
      781 ccgaagaagt ctgtattacg gtaagtcagg acaccggaag ttcgggccgt cataaagtta
      841 tcggtctgcg tccagccatc accaccaaac tcaggcagta cgtcagtata tgatccgaca
      901 tcgtaagcaa taccatagtt acgtccgtaa tcaatggtac caacatctgc aaatctcaga
      961 cctgcaaatg ccagacgggt tttatttccc gcggaacctt cagattccgt tttgttacca
     1021 gagaactggt attcccactg accaaatccg gtcagttgat cattaatctg cgtttcgcct
     1081 ttaaagccga gacgagcata agtctggtct ccgtcattac ctttatcatc cgagaagtaa
     1141 tgcagagcgg ttgcacgccc gtaaagatcc agtttattac catctttatt ataaacttct
     1201 gctgcctgcg cccccgcagc aaacatcact gccactgcta cagcagaaag tgccactgtc
     1261 atttttttca tgatttaatc cttatttaaa ctgaactatt catgcattta gatgtcatga
     1321 acaaaaatta aaatatttta atacaaattc ttagttcaat ttatattatg taacaaaatg
     1381 taaatacaag atcgatttcg cactttctga caagacagat taaaaataat taacaactaa
     1441 acaataaaga gtgatttata ggataattct catctgacta cctgctctat cgtgcaactg
     1501 aaatcactgt accgggcgtt atccgtaata ctccactccc ggcacaccac cctgaccgtc
     1561 cggttatgtt tcgggggctt ccacaaaaaa gcccggtaac caccatgcca tgacagaaac
     1621 gcggacagtg cctcccgctc agcatccgtt gtcacccgaa aaaccacctg aaaggtcttc
     1681 agttgcgcat tcagtcctgc cggacgacgt tgctgataac cgtcgccaaa cttcaccgtc
     1741 accaccgacg gtttctccgt cacctgcatc ccttcccggg gacaccagtg aagggtctta
     1801 atctcatcca ctcagcattc ctccgtcacg acgcatggat aacatcaccg cctgtacccg
     1861 ctggtcaatc agttgcacaa gggtgcccgc cgcttccggc cctatctgcc cgttagcgcc
     1921 gtcattctga atggcaatgt ggtacaccgg ggaatacacc agacccgcac tgccgttcat
     1981 actgcccacc gcacgcacgc cgagtgaacc atccgctgcc cgggtcaggg gcatgatggc
     2041 ttcaggtccg gcttccccca tcagccctgc cccttttgca aaggcaaagt acgtgggcgt
     2101 gtccacaata ctgttactgt atgcactcag gtttgccgag gtataaacgc cgccttttgc
     2161 attggccacc gcgccaccca gccagttgcc tatactgccg aaaaatcctc ctgcaccgga
     2221 catactgttt gcggccatct taatgccgtt gacaatggcc gcattcataa gaacttttga
     2281 tatttcctgc aggattgatg cagcccagct gcgccattcc actttatttc cgttcagcat
     2341 ctccgtgatg ttattcacca tccctgagat accgtctgtc gcaagctgtg ctgcctgtga
     2401 agcgtaatct gatgcgctgt ccacccagtt acttagccct tcctgcagcc ctttctgcca
     2461 gtcagcacgc tgcgcatccg attcggcata aaaagcctcc tggtctttaa ggcgttcact
     2521 cagatactgc gcattctgcg ccagcgcctg tctgtaaaaa tcctcactga tatcccctgt
     2581 ctgatactga gactgcaggt ccgcatcctt ctggcggtag ccgtcacgaa tctgctgcag
     2641 ctcccgcatc cgttcacgga cccgctcacc ctgcccgtac cccagcagct cagcgtcatt
     2701 tgatgccctt tcgtctgcat tatcattttt cagagcctcc tcccgggccc gtaactgctc
     2761 ccggattttc ttctggtcaa tcagggcagc attgcgcagc aactcctgct tctgtatctc
     2821 cgtcagggtt ttcagctctc cctgcgaggt ctggtacttc agcttcgcca gttctgtatt
     2881 cttccctgcc agtgccagtt gttccttctg ctgcttcagc agacgggaaa aactgtcttc
     2941 cgctttttcc gtctcagatt ttccggcccg ggatttgggt ttattcgcct cattattgcg
     3001 ccaggcttcc atcgaattac ggatgtaacg ctgcctcgcc tcctgatacg tatcccccac
     3061 cagaccaagg tcatccgccg cataccccag ccgggcacgc tctttttctt cccctttcag
     3121 ccgggacagg gccagttgac gctctgtgtt attcagggcg ccctgctgtt tatcatccag
     3181 agtggcctgt ggcagtcgca gcggtacact cgccagtccc tgacgctgtt gcagcagttc
     3241 attacccagc cccagcaggc ggttgaattc cgtatgctga ccgttcatga tcagcagtga
     3301 ctgatacgct ttattctgct ctgccgcctg ctgacgaatt aacacaacac ggcgctcttc
     3361 cagtccggca agcacatcct gaatggactg cgctttttcc tgcatctgtg ccagacggga
     3421 ctgctcaaca gcaagctgct ctgttgcctg agcaagccct tccgttacag ttttcaccga
     3481 tgtcaggtgg tttatcatga agccgttacc ggtcgtccag cccggattag acagcacata
     3541 ctgatacccg gcgatttttt cctgcaggga tttcacccgg ctggcctgct catcaatcag
     3601 ccggttctgc tctgtcagcg cctcccgtgt tcgctcttca ttatctgagg cttcaggcag
     3661 agacattgat ggcgttttat gcgcgatttc atcaatcgtc agtgcatact ggcgcgcaga
     3721 ctccctggcc tgttcctgat tctggtacag cgtgtaccat gcggcagctc ccagcatcac
     3781 cagtccgggt acgcctccaa ccagtcccag cgcaccgctc atcagacgtg agcccaccgc
     3841 cgttgtactg ttcagcgcat tctgggcggc ggttctggca gcaatatttc tgttcaggcg
     3901 ttcctgtgtg accgccagac gggcctctgc cgcaatctgc atctcagtcc cgcgggctgc
     3961 cgccacagcc tgctgagccc ggtacacggc tgcccttgcc cgcgccgtgg caatctgcgt
     4021 tccccttaac tgggcctcag ccagtgccac ttcattacgt gcagcggtca caagtcctgc
     4081 cgtggcagac atcgctccgg aagccagatt accaaagtac cgggccaccc cgacagcaac
     4141 cagcacaccc gcagctgttg ccacggtatc aatatttccg gccacaccgt tcagtgcgcc
     4201 agagagcgtt ttcgtcgctc cgctggcctc attcgcgccg cccacccagg ccataaaggc
     4261 gttttccacc ttcgtgatcc cgtcagacac cgtttccggc atggctgcat attcatcacg
     4321 caatatcccc agctggctga ttaacgcggg aacgacttta tccgccgtca gttgaccatc
     4381 gtccgccatc gccttcagat ccttacgggc cacgcccatg cctgcagcca gtgcgcgaat
     4441 gatccggtcc ccactttcat tgaccgaatt aaattcctcg ccgcgcaaca ccccctgtgc
     4501 cagcgcctgg ctgaactggg tgatcaccga acccgcctct gccgcactgg caccggaaat
     4561 tttcagccct gtcgaaatgg cctccgtcac cttcagcaca tcatcagcac tgtaaccata
     4621 ttcacgcatc gaggcagccg aacgggcaaa cagggccgca ttatccgaaa atgcggtgcc
     4681 tgtccgctgg ctgatatcca tcagcacttt ctgtgatgac gaaaattcat ccgatgactg
     4741 cgacgcctgt ttcagacggg cattcacgga gctccattca tccgccagcg aaatcaggtg
     4801 tccggtggca aaggcaccag caaacgcacc ggtcattccg acagccgaag cgcggatttc
     4861 cgtcaactgg ctgttcagct cagccagagc ccggcgctgc tccctggctg ccgcagcagc
     4921 ctgacgtccg ccattctgca gggtccggta atattcactg cccatacggg acgcccgctg
     4981 gatctccgac tggaatgact gtgaatttgc cgaaattttg ataatcagtt cacgtaacgt
     5041 cgccattcac ctttctccgg gcgaaaaaaa acctgccgca gcaggtttta ttcatcagca
     5101 gattattgat taagttttgg gtaccagccc gggattcaga ctttccggac tcagtgtata
     5161 cataccgcca ttgaatgggt ccacaagcaa ccagccaaca ataccaccaa agacaaagtt
     5221 accaccaatg taccatccat tagccgatgc cttgatcggc agcgttacgg gctgataatt
     5281 ctctttactg aatgtgatct gataactctt tttcccgaag taactgccgt cagatttttc
     5341 aagtgttaca ttctgcggtg ttttgcccga agaaattgtt ttccctgact catctttaat
     5401 aataaaatcc gcaccagacg gattactgtt tacctgtaca gtctgcgttt tatctccgac
     5461 aatggttgca caccccgtca gaaaaaagct gacagagaaa atcaacagca tgaatttctt
     5521 cataaaatgg ctccgttcag ttaattttcc ccgaaaatac tgtaataaag atctgcaaag
     5581 ttaaaattaa ttttccgaag caaataacag agcatcctcc agagcggcca aaggatctgc
     5641 ctccggctta tcctcatcct cgccccagca gagcatggcg tccttcagtg caacattcat
     5701 cccctgtgcc ccgaaaaccg ctttcacgat ctgtgcatta cggatatccc cgcgctcatc
     5761 acccagcggg gataccctgt cgaactccat ccacatcatc gcctcgctcg cactcaggct
     5821 gtggcgcagt tcggataagg tgcgccccag acggagcgca agtcgcatca gaaagcgaat
     5881 ttccgggcgg gctacttttt tctggccgac tctgcatcag cgatcagttc cagtgcctga
     5941 cgcagcaagc gggcatgtac cggaccatag acggccagca cctgctcacg gtcgtccgga
     6001 gtgaacaccc gctgcaggtc cgtatcacac aggacatcgc agaacagcgt cacatccgct
     6061 tccaggttac ggcgggtttt cgccaccacc gacagggtat cgtcatcctc tccatcacca
     6121 ttgagcacgt cctgccacag ataccaggcc tctgccgaag gctcccgcag caccacgctg
     6181 acattacccc attccggcac cttcaccgtt ttatgacgaa accctgacag tctggccagc
     6241 gccagcgttt tcagatcctt tttcatgatg acccatcccc ttatccggcg gctgcgctca
     6301 ctgtcactgt acattcaaca gacgtcacac tctgtgcttt ctctgccgaa tcagtcacca
     6361 cgcaggtata tttccccgca tcagcggact gcgcacctgg cttactgaag gtgtctgtcg
     6421 tctgcccgtc aaccggctga ccatccttct tccaggcgta tttatacggc ggcgttcccc
     6481 cgttggcact gactgacatt gtcagcagcg caccggtatt cacggtaagt gtcttatcca
     6541 gatttttcac aaacgccagc ggtaccacaa aggacaccgg tttgcctttc agacgcagtg
     6601 agaacgttgc agccaccacg ccgttggtac cggatgacca ggtgtgctgg cgcacttccg
     6661 ccaggaattt aaagccctta ccggacggaa actgcacctt aaacgcatac aacgcgtcat
     6721 tgtcataagc atcacgcagg gcgttctggg cctgattcag ataaaaatta cccgacatgg
     6781 aaatctcgga cgacgccccc agaccattga tattctcctg ctctgtggag cagagcgtgg
     6841 tcacatcaat atcctgtttc tgaccggcgg tgaactggac ttccttgatg gtgcagtcca
     6901 ggcgcagata ttccgcctta tccatagttt cagcagtcgc cggggcagat gaaatcatca
     6961 cctgcgtcag ctgtgagcgt tcatacaaag cagacattct gcctcctgat aataaaaaac
     7021 ccgcacgcgg cggggtatgg gttttgtaga aaaaagaaaa agtcacaccg tgacctgaaa
     7081 ctccagggtt gcacggtaac agcggttttc cggaatatag tcctgcattt cactgacgga
     7141 tcccggggcc agcagcatta tggcttcacg ggcgtcctga cgtatctgac gcgcctgcgt
     7201 cacagtcccg gcataaacgt ctatctgcac cgccactgag gactccgcct gcccgcccat
     7261 cacgtccgca gacaccgatg aaatcaggct gaaaaccacc cacggaagcg ccaccgacgg
     7321 cctgccatcc agcaggggga ccacatacgg gtacacctgc ccgccggcaa gatgcgccag
     7381 atgaggatac aaatccgcct ccgtcatcgt ctcagtacct catcaatggc ccggttcatc
     7441 cgcgcaatcg ccacctgcgc tgcctgttca ctgcgcacat caaacgccgg gcgcacaaac
     7501 gggtgcggtg gcatattcac ggtccccatt tccacaaacc gccagtagaa agcattccgc
     7561 gggttatctg ccttcatggt gttatcgctg ttaccggtgt ccggattaac accacggata
     7621 tggacaccgg attctatccc gccatcgcgg gagcgccggg aaaggaccac cacattgcgg
     7681 cgcagttttc ccctgcgcac cggtgcccgt gacaccactt cttctttcag cacattcgca
     7741 cccgcacggg ttgcctcacg cagcacccgg ttattttccg caccactcag aagctgcaaa
     7801 tcgcggctga tgtcctccag ccccgaaaaa tccagcaggg tttcgatcat ttttcccctc
     7861 ccagccgaca gagaatttcc agacgcccgc cggtcgcatc cggcacgggc agcccgacaa
     7921 cgttcaggat ccggtcacgc catggaccac tcagcacatg aagtcgtgac gctgccgtga
     7981 tttcccgacc ggactgaccg cgcacccaga tgcggatttc cgcctgcgcc atttccgcac
     8041 cggactgcat ccgctcccgg ctgctcctgc cacggatatc cgcatgaatt ttcccgcatg
     8101 acacccattc ttccgtcatt tctccggcag cattacgggt taacaccggg ttcagaacac
     8161 ttatcatctg tgtcagacga cctgcagata ttgccattcc ccctcctcat aacaccgtcg
     8221 gacaacgcaa atcgtaaatc agcacggaaa cagaaaacgg cagctccccc tgaagcagtt
     8281 cttcccgctc cgcaagatcc ggattccggt acagcatccc ggtcagtcgc atggcagccc
     8341 ccttcatccg ggttaatgcc tcgcccggga tcagttcacc gtcctcacga atcaccttat
     8401 cccggctgcc ctgaatgtag gccagcagca cggcggtagc ctgacgaacc ttgtccatca
     8461 gcatgtcatc atccgcgtca tggtcgacac gcagatgtgc cttgatctct tccagtgtca
     8521 gtaatgccgt cattttccac ctcctgcatc ccgcccacgt tttgcagcca gggtccaggc
     8581 tgatgaatga gcttctccgg gtttatcacc ggtcatactg ttgcagtgcc acagcgagcc
     8641 cccccatgtc accgtatcgc cggggtggta ggtttcaccg gctctgaaca caccgcggta
     8701 gagcatcacc ggcagggaaa atgttttttc cgtacgctgg ccactgctct gccggaccac
     8761 cacagagaac aaccgttcac ccgtcatgct gacgtcaata tccgccaccc cgtcaaccag
     8821 gcattcccat ccccgcatcc cgtgcgtttt ttcatacgcc cgccagagtc cgccctggtg
     8881 tgtggcatac gtgccccggg gaaaggattt ttgatcgtca atggcaggga gtatttccag
     8941 tgccgtggca tcacgcccgt cctgcggagc cggcagggca ctcaccgcat ccagaaccgc
     9001 cttctgcaga acatcgggat catagtcacg accatcacgc ggaacaggaa tatggcttac
     9061 cgcctccttc accatctgtt caagcatcgg acgcacatca tccggggtga tacttttacc
     9121 gtccgccggc tgcggaatat ttgcgaccgc atcattcacc gccttctgca gaacatcggg
     9181 atcatagtca cgaccatctc gcggaacagg gatatggctt accgcctcct tcaccatctg
     9241 ttcaagcatc ggacgcacat catccggggt gagactttta ccgtccgccg gctgcggaat
     9301 atttgcgacc gcgtcattca ccgccttctg cagtacttcc ggatcgtagt cacgaccgtc
     9361 acgcggaaca gggatatggc ttaccgcctc cttcaccatc ttctcaagca tcggacgcac
     9421 atcatcgccc gtcacgcact tctgtaatac cactgacagg gacgccagtt tttcttcaaa
     9481 cgtttgtgcc tgcgcggcta ttttcccctc aaatgtgcgc tgtaaatctg ccagcactgc
     9541 ggaaaattct tcacccagcg cacggataat ggacagttca cgttcattca ttttttcaga
     9601 atccccctga acatcgcctt caccgcatca tgctctgttt cagtgattgc cttattaccg
     9661 tcagaggcag cgaccgcctg aggcacggaa gctgttttac cggacgacgc gaacggatct
     9721 tcacgggcat cacggcggga caacgcttcc agactgtagt tctgctgctg aagatacagt
     9781 gcatcaccgc cggccagggg cggcaggttc tcccgtttac gggcctcatt gggcgtgaga
     9841 agcgtatttt tcaccgcatc ccccagcgtt ttcatgcgcc gctcactgtc cattctcagc
     9901 agcgtggtga catcaaattc cgtactctcg ttttcccccg tttccagcgc ctcatccagt
     9961 aacagctcaa tggactcaat cagtgtctgc aggcactggg aataatactg ctgctccagc
    10021 gcctccacgt tgtcactgga aggcggttgt cccacgccaa tcttgtaggc cgggacacgg
    10081 aacaccgaac agacaatttc agcggtcatc ttcagttgtt ccaccgtctg tgcatccacc
    10141 ggtgaaaacg tcgtggggtt gtattttgcc ccgttgctca gaatggccgt tttccccgca
    10201 ttttcgcctg tatacccgct gtcccagttg ctcttcagtt ttttcgcatt ttcttccgta
    10261 atactgccgg ggatctcaat caccccggac ggcctgccgc catttctgaa aaaagacgtc
    10321 gaattttcct gaatatgatg cccctgcgtg gccgccagcc cggcggcata caccggcggc
    10381 aaccctataa gcggatgaaa aaaacagtta aaccggtcgt ggatcacttc ccgggcaggc
    10441 accgtcaccg cctcagggat cccgcagttc cggtccggcg tgatgcggta gaacacctcg
    10501 ccgtcatccg ccaccagagg ttcaacccgg ttccagtcca gaatacgcag ttctttgatc
    10561 tgcccccggg cattacggat tttcagcacc accgtattgc cgtgacgcag tttggcgttc
    10621 agccacagtt caaaaaactg gatgcggttc tgctgggcgt tgggacgacg acagaggcgg
    10681 gcaatatccc cccggcgcgt ttccctgcgt atcccctgcg catccgtctg catcaggcgc
    10741 agccgcattt tggcgatatc ctgggatatc agcgaaatac atgcaaacac cgcatgaaag
    10801 gagaggacgg cttcaggatc ggctttcacg ccctgctgcc aggcaccgga aaagggctca
    10861 gccaccgcct gaaacaggct ggtccagccc gcctctctta cgtcacgtcc tgatttctgg
    10921 ttttttcggg ttcgccgtaa aaggttccac attcgccatg ctccgcatca cgtttctttt
    10981 tctgacctgc cggacgttgc accgtgatgt actccgcctt tcccaggcga accagcacct
    11041 ccgcacacgg ctgtgccaca tcacggatat ccccggcccg ggcatcatgc gtgccctgca
    11101 gatatcggat ctttgccata acctgttacg ggaggcgcac gcctcccgtc ctccttatca
    11161 gactcagccg ccggacgcac tgccgtagtt cactccggtg atcaccgcca ccgccgcggt
    11221 acggcgacga cgccagttga tccagcgctc cgcacggatg gccacgctgc ctgtctggaa
    11281 catggaaacc agctccaccg gcgacggcgt ggtactgtcg ccggtcggct cagactgcat
    11341 ttccagtgat gcctcgcggg acatatccac tgccacgccg ccgtcatccg ccagataaat
    11401 atccggggca ttcaccagca ccagctggtc acccacgtac tgggagacaa tcaccggcag
    11461 cccctggaag gagccaccca gcagggtcat gtccgggtat tctttctgac ccagcgcatt
    11521 tttacgcatg gacagtgcca gggcattggt gctggacatc agccagaccg caccggtggg
    11581 ttgcaggttt gctgccacaa actgtccaaa cgcagcctct gcatccgcat ccgggttacc
    11641 gcttgatgcc gtgcccttca catcatgggt gatggacgcc ggggagacat ctgccaccgc
    11701 cgcttttttc gggtccacaa agtctgtatc cagacgcgcc accaccgctt ccgccagcgc
    11761 attacggacc agtgcatcag cagccggact ggaaaaacgg atcaattctt ccgtcagtac
    11821 cgcaatggcc gacaccttcg catgactgaa ggtgatggat tcaaaatcaa acttcgtcag
    11881 gggttttgcc ttaccctcac ccacccagcc ggcagcaccg ccggacacct gggcgtgcac
    11941 acggatattg aacggcacct gacgaagtgc agggatcccg ccctgaccaa atcgcccgat
    12001 aatggtctga ggacgcaggt aatcaataaa gtcctgtgcg tattcctgat attcagacag
    12061 gctgcctgcc cactgcggat ccgtggtggt ccccgcgccc actgccgatt tcaggacatg
    12121 atgcagacga ctgtcatccg gatactgacg acgggccact tccagggctt cagagcggac
    12181 acctttagcc gcagccagcg atttggcaaa acgtgcgaaa ccaatcccct tatccagttt
    12241 ctgctccaca cggatcaccg gcgcagaagc caccgcggcc acattcccgt taccggcctg
    12301 tttcaccggc tgcgccgtgg cggccttacc ggcttccagt tcacgcaggc gcttcaggtg
    12361 cgcatccacc tgacggattt ccgctgcggt gttgtcgtaa tgctcttcct cctccacatc
    12421 cagcgtgcgc ccttcctctg cggctttggt catgacctcc tcaagggagg ctgccagcgc
    12481 tgcacgcttg ttttcaaaac ttttaatctg ttcgccaata ttcattatgg tcttttcctt
    12541 atgaaaaacg gttgttgact gtgccgcagc gccggcagaa gatgcgattt tcaccaccgg
    12601 tttccggttg ccggacgcgg cagaaaacgg gcggtcgtaa gatttaatgg tccggatagt
    12661 gcattccgca ttcgcgggca cggtgacggc agacacctcc atcagctccc agcgcagaaa
    12721 atgcactccg cctccgtcca gaaaggtgta ttcatgagga cggaagccca cggacagccc
    12781 cctgaccagc ccggtcttaa tggccgccca gacctcatcc agccgggcag ccagttgcga
    12841 cggcatatcc ggtacgggtt tcaccagtgt tgccgtgatt tccagccctt cgctgacccg
    12901 gcgcaccgta cactgcccca ccgggcggga atggtcatgc tgccagagaa acgggatcgc
    12961 actgccaaac tccgcgccct ccggctccag gatgtcacca tcccgatccg gagaaggcgt
    13021 tgacgcaatc ccggtgatca cccgctcatc ctcactgaag gatttcaccg tcagcaggga
    13081 gcaggcccgt ttaagagtca catcagcctc ctgaaaataa aaaaaccgcc gcagcggtcc
    13141 atgatggtta cagggtgaac agggttatat gaaaaaaacc gcatattctt tctttttggg
    13201 ctccggattc agggacatca gggataccgc attgaacagc gccatcagcg ggtcaatttt
    13261 cccccgtccg ctggcctgtt tggtaataag gatggcgtta cctttaggct ccacccgggc
    13321 attgccaacg caccaggcca tcagtggctg accaccatgc accagcactc cctcagc
//
LOCUS       DAZGBC010000137        13278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGBC010000137 DAZGBC010000000
VERSION     DAZGBC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.138"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..171)
                     /locus_tag="ACHK1J_003813"
     CDS             complement(<1..171)
                     /locus_tag="ACHK1J_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7913028.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPV"
     gene            388..723
                     /gene="iraM"
                     /locus_tag="ACHK1J_003814"
     CDS             388..723
                     /gene="iraM"
                     /locus_tag="ACHK1J_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO7913029.1"
                     /translation="MKWIVIDTVIQPSCGISFSAIWSKIKLIIWYQSDVFLPPESIFT
                     LTHTGIVLNNKVPPVTIYNVVPFNKRFWDLIKNSQECPANSDNVLKECFNNHCILQIC
                     PYGLKQQCP"
     gene            complement(1025..1189)
                     /locus_tag="ACHK1J_003815"
     CDS             complement(1025..1189)
                     /locus_tag="ACHK1J_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7913030.1"
                     /translation="MTVKLRLAAVALLLFLVVMVDFTSRIMSVLADGVLVVGIVVVLF
                     PLVKKDMPGS"
     gene            complement(1186..1617)
                     /locus_tag="ACHK1J_003816"
     CDS             complement(1186..1617)
                     /locus_tag="ACHK1J_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7913031.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATITGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(1807..2016)
                     /locus_tag="ACHK1J_003817"
     CDS             complement(1807..2016)
                     /locus_tag="ACHK1J_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913032.1"
                     /translation="MFVSEVLMEDKDNKGWVKGWAVVRSSPWHLVGVFATEEDAETEA
                     RKMGDKYEVHYGSHRTGSDDFVWGE"
     gene            complement(2069..2293)
                     /locus_tag="ACHK1J_003818"
     CDS             complement(2069..2293)
                     /locus_tag="ACHK1J_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913033.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            complement(2383..2459)
                     /locus_tag="ACHK1J_003819"
     tRNA            complement(2383..2459)
                     /locus_tag="ACHK1J_003819"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2423..2425),aa:Arg,seq:tct)
     gene            complement(2473..2549)
                     /locus_tag="ACHK1J_003820"
     tRNA            complement(2473..2549)
                     /locus_tag="ACHK1J_003820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2513..2515),aa:Arg,seq:tcg)
     gene            complement(2559..2634)
                     /locus_tag="ACHK1J_003821"
     tRNA            complement(2559..2634)
                     /locus_tag="ACHK1J_003821"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2599..2601),aa:Ile,seq:cat)
     gene            complement(2675..3732)
                     /locus_tag="ACHK1J_003822"
                     /pseudo
     CDS             complement(2675..3732)
                     /locus_tag="ACHK1J_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(3883..4080)
                     /locus_tag="ACHK1J_003823"
     CDS             complement(3883..4080)
                     /locus_tag="ACHK1J_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7913034.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(4296..4676)
                     /locus_tag="ACHK1J_003824"
     CDS             complement(4296..4676)
                     /locus_tag="ACHK1J_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7913035.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(4694..5683)
                     /locus_tag="ACHK1J_003825"
     CDS             complement(4694..5683)
                     /locus_tag="ACHK1J_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7913036.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRLAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(5735..5993)
                     /locus_tag="ACHK1J_003826"
                     /pseudo
     CDS             complement(5735..5993)
                     /locus_tag="ACHK1J_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5990..7390)
                     /locus_tag="ACHK1J_003827"
     CDS             complement(5990..7390)
                     /locus_tag="ACHK1J_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO7913037.1"
                     /translation="MTNPTNPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWAKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMTSLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRHIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTQGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKAERLF"
     gene            complement(7387..8277)
                     /locus_tag="ACHK1J_003828"
     CDS             complement(7387..8277)
                     /locus_tag="ACHK1J_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7913038.1"
                     /translation="MTNFLNKQHMQHDKAQLLNRKADLEEELAFVRGGKRPWRCEHWV
                     QSTETVSCGKHGKYTRYVLTGPDVRGKAVKRVSGCLSCLSEELDRVYDELRALKVREL
                     LDQAGIARRFQDCEFENYQAVNTDAQKNLEACQRYAASWKKCLAAGTSMVMIGNCGTG
                     KNHLAVSMAKNIIRHHQATIEITDVMRLTRAVKNTWRHNSERTDDEVINHFASLDLLI
                     IDEVGVQFGTPTEITILQEIINARYESVLPTILISNLTFEQLKESIGERIVDRVTDGG
                     RNCLVFGWESYRAHIRGVAA"
     gene            complement(8297..9208)
                     /locus_tag="ACHK1J_003829"
     CDS             complement(8297..9208)
                     /locus_tag="ACHK1J_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7913039.1"
                     /translation="MSSKILGNVWDACAAYGIKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLELLARTERD
                     KVAELKQQRRLSGLRDPSDSEPSKSEPSESVCSGVFDPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEESWQQPA
                     APGGGRGKSLPISGFSEQDYGSTNFNW"
     gene            complement(9205..9429)
                     /locus_tag="ACHK1J_003830"
     CDS             complement(9205..9429)
                     /locus_tag="ACHK1J_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913040.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGHVLRVCLVNIESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            complement(9426..10289)
                     /locus_tag="ACHK1J_003831"
     CDS             complement(9426..10289)
                     /locus_tag="ACHK1J_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7913041.1"
                     /translation="MTRHQHIDSASPGRYTVPAPYKTGAGIGVLKLITEHNRAHAVFS
                     CHEHYYAQIMVGRTGPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMT
                     TLPTLSQPEIAIVDGQAVTSSLAVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSI
                     LVRQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMEKSLSGADAS
                     DMSAVARNARGVYLHLREIHQIWTSQLYPMLKAVESPLASKLYDRVGDAVFGAALVDS
                     RLNGSDKEVRP"
     gene            complement(10286..10918)
                     /locus_tag="ACHK1J_003832"
     CDS             complement(10286..10918)
                     /locus_tag="ACHK1J_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO7913042.1"
                     /translation="MKFRRHTLHIVAKSTIGSETPELLQATNDAPASFLLSASLHARI
                     MVGWAGEPKGSPVSCNAGSLNPVQSATSEIETSGGGTVPLLQEAALMATIPAHPYLKI
                     EVVNGRAVIFSLYVACHFKRMHKNIIDRIEYLNCSREFLTRNFIPGTYRAYGDSVRGY
                     YITRDGLMMLQLGLSLRTMRYYESCIEAFHEAEAVQGHSVFRRNQREVHQ"
     gene            complement(10947..11123)
                     /locus_tag="ACHK1J_003833"
     CDS             complement(10947..11123)
                     /locus_tag="ACHK1J_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001664303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7913043.1"
                     /translation="MQVLNRWFRDGRGRRVHVIRWESETQRVIYLRDGYPHECFIPLW
                     LFRRDFVECEAPPT"
     gene            complement(11143..11850)
                     /locus_tag="ACHK1J_003834"
     CDS             complement(11143..11850)
                     /locus_tag="ACHK1J_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7913044.1"
                     /translation="MNTTIFTDKASMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQHEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRLERVEEKVSHVTETVEQIKRGAIRDGYAGYRQLAAK
                     TGMSDAKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMSAFHRMTSEAEPRGT
                     RWYHPKMGLFQALGWQV"
     gene            complement(11847..12110)
                     /locus_tag="ACHK1J_003835"
     CDS             complement(11847..12110)
                     /locus_tag="ACHK1J_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7913045.1"
                     /translation="MKNEGIAKAVDIAGSQIALARRCGRAQSTICDWLNGKKKISPEF
                     VPSLVKAVDGKVQAYEFRPDLPELFPHPSLAHTEDGVCEGDKQ"
     gene            12234..12938
                     /locus_tag="ACHK1J_003836"
     CDS             12234..12938
                     /locus_tag="ACHK1J_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7913046.1"
                     /translation="MSFSDRLQLAMTIRGYSQGKLAREVGMAQSSVNKLVNGATGSRK
                     VVEIANTLNVRPEWLSYGVGPMTPEESTSCNPTPQVSYSPLENDKDIFRVEVLDIAAS
                     AGPGTFLVSDFAETVHAIEFSHDAARRLFCSRPANIIKMITVDGDSMAPTLCAGDQVF
                     VDVSVRNFETDGIYIFIFGHTFHIKRLQMQGMQLAVISDNPAYKEWFISESAEEHLFI
                     MGKVLIHQSIQYNRVG"
BASE COUNT         3322 a         3624 c         3022 g         3310 t
ORIGIN      
        1 cacaggtgtg gtgacatcac cttccgccgc aatggcctgc atcagggtat aaggcgtgat
       61 ggcgacaggg ctgccaaatg gctgccaccc ctccttcagt ttttgagtca gtcgctccgc
      121 aaggtctgac ggcgacgccg ccctgaccac atcgtagtgt ttaaatgcca tggttctttc
      181 caccatatta aaaacaattc tttaaaatac ctgacatgca atacagaaaa aaaacacaaa
      241 accatacctt aaatgaaaac ctcatcataa agcaggtatg catggataaa ccacatcaga
      301 aaaataattc tgctctatgg tttacattca aaattatcat ttatactttt cagaacatca
      361 ccagcacagc ataaataagg aggctaaatg aagtggattg tgattgatac agttatccag
      421 ccatcatgcg gaatatcttt ttcagccata tggagtaaaa taaaattaat aatctggtat
      481 caatcggatg ttttcttacc tcctgaaagt atatttacgt tgactcacac aggtatcgta
      541 cttaataaca aagtgccacc tgtaaccatt tacaacgttg taccattcaa taaaagattc
      601 tgggatttaa tcaaaaacag ccaggaatgt cctgcaaatt cagataacgt attgaaagaa
      661 tgctttaata atcattgcat tctgcaaata tgcccttacg gactaaaaca acagtgtcca
      721 taattagttt actcatatct tacaaaattc atataaaagc ccctccggag aggggctaaa
      781 gccgcgtatc tgtatcatca tgcgcatgat gccgggtgcc tcccggtgag ttcagcccgg
      841 tgccaccaaa cccgcgcgtt cactttcctg ggtatcaaag ataacaccta atgcaccagt
      901 cgcccctccg ctcaggggga ttcaccatgc cagtttcttt taacaaactc cccgcaaaac
      961 agacaactgt caaccgtctg aattgtgaga catttaaaat tttcggggca tgactgatac
     1021 ccggctaact acctggcatg tcctttttca ccaaaggaaa aagcaccacc acaataccga
     1081 ccaccagcac tccatccgcc agcaccgaca tgatcctgct ggtgaaatcc accatcacca
     1141 ccagaaacag caggagtgcc acagcggcca gacgcagttt taccgtcaca ggtggttctc
     1201 cagtcgcagg ccaagaacac cagcaatctc ttccagaacc ttacgctctt ccggctcaat
     1261 ttcaccatct gcttcagcaa tagccaccgc cacatccagc acatcttccg cttcacgcgt
     1321 atcgtgtttc acatcttcaa tttcacgcaa cgccgctcga cgaccaattt taaagttggt
     1381 gtcaagctga cctgtgatcg tggcactgat ggcattaatt tctgacgtaa acgcggacaa
     1441 cgcaggctgg ttacgtaata cctgttcgat cttcgctttc tctgaagcct cacattcacc
     1501 atctgcatag gctaccaggt aggcagcatt aataaccgcc tgtgccagat cgcgtttctc
     1561 aaacttttta atttccgttg ctgctctgcg ggttttcttt ttgaaaatac caaacatcgt
     1621 gacgttcctt tgggtgggtg agccaacgcc cgggagcgat ctgcccacag agaaagtcac
     1681 actgaccact ccgtaagctc acccccgaaa ggctctgtgg ttgatatgcg ccgggcgtgg
     1741 cgcggataca aaaaaggccg ccaatagcga cctcagttac gggattattc tggggttaaa
     1801 cgactgttac tccccccaga caaaatcatc acttcctgtt cgatgcgagc catagtgaac
     1861 ctcgtactta tctcccatct ttcttgcttc cgtttctgcg tcttcctctg tcgcaaaaac
     1921 cccaacaaga tgccagggcg agcttcttac cacagcccaa cctttaaccc atcctttgtt
     1981 gtccttatct tccattaaca cttcagaaac aaacatattt atctccttgt gggtacccag
     2041 agatatttta tgattgctcc cggtcagatc aataaagtgg cttcaatttt gccttaatga
     2101 tcaaatcagg gtgattgacg gaatcgtaca ccacctcaat attttcatcc gtggcgtcga
     2161 taagatattc ttttacataa ggacctgttg attttccatg aaatacatct tcaacaagta
     2221 cactctcccc ctgaacaaca cgaaaactaa cttctgtttc gaacggacca atcgtcacca
     2281 tcagtttttt cacatagcct cctgataagc actcgattta ttagttaatg gtgtaacgca
     2341 gatacaaaaa aggcccgcaa aagcgagcca gaaaaataag tatggcgcgt tgtactggat
     2401 tcgaaccagt gaccgattgc ttagaaggca attgctctgt ccggctgagc taacaacgct
     2461 gaataccgat aatggaccgc catcgaggac tcgaaccccg cgcaaccagc ttcgaaggct
     2521 gacgctctgt cccgatgagc taatggcggt atgtgatatg gtggcccttg ctggatttga
     2581 accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag ggccgggtgc
     2641 aggataataa cggtacgtaa ctaatcctgc aatatcatcc gttctgactg gctaaatcct
     2701 gaacttccct gaccgtctgc tcaaaacgtt cagtctccag ctcaacacca gttgcacgac
     2761 gccccagtgc catcgcggct ttgactgtcg aacccgaccc catgaaaaaa tctgcaacca
     2821 ggtcccccgg acgactgctc gcgctgatta tctgctgcag catttctgcc ggtttttcgc
     2881 acggatgttt cccgggatag tactgcaccg gtttatgcgt ccacacatcg gtgtacggca
     2941 cctgcgccgt cacgccaaaa taccgccgca gatgcttata ttcactctgc agttccgcat
     3001 actgccggtt cagtgaagta tacgtctcca gcagctggtg gtggggcttt tccagtacac
     3061 cgcgctgatg cttctcttct gccacccggg caaacagcga ctgtaatttc agataatcgc
     3121 tttcgttcgg tagctgccac tgactggcac tgaaccagtg cgacaccatg tttttctttc
     3181 ctgtggcatc cactatctgt tttgccgtta tccccagggc agcacgcgca tcacgaaagt
     3241 aagcaatcag cggagccatc acatgctgtt tcagtgccct gcccttcgcc tcatacccgg
     3301 catctttcgg acgatacggc ccctgataat gttccgcgaa cagaatgcgc tctgtggcgg
     3361 ggaaatacgc cctcaggctt tccttgttgc acccgttcca gcgtccggac ggctttgccc
     3421 agatgatatg gttcagcaca ctgaagcgtt cacgcatcat gatttcaatg tcagacgcca
     3481 ggcgatgacc acagaacagg taaagacttc cggcaggttt cagcacccgc cagaactgcg
     3541 caagacactg gtccagccat ttcaggtaat cctcatcccc cgtccactgg ttatcccagc
     3601 cctcgggctt cactttaaag tatggcgggt ctgtgactat cagatcgaca gagttttccg
     3661 gtaaggtctg ataaattcca ggcaatcagc gttgattagc tcacaactgg atatttttac
     3721 agtattagcc atagatcaat aagcacttct ctgataggct cattctgctt ttgcgcaaag
     3781 cagtgggcct gaggtttgct tgtgatccgg acgcatgagc agatggctgg tgagtgcccc
     3841 taacacccac cagccgccca tttaccacaa ataaaaaagc cttcaggact gaaggcgtct
     3901 gtaacaaccg aactgatagt ctgccagccc cgccataaca agctgggtca gtattaactg
     3961 gcagcgttca cgtgaaaggt acgtattctg cgcaatctcc ccgactgtcg ccggttcggt
     4021 gacgcttaat tcattaaaca ccactctggc ggtttctgtc atatcctgct gtttcagcat
     4081 gtctttttcc cttttccggt taacgtgaca caccaataac tcttgtcgaa aaagccagca
     4141 agctgaaaga ccggtattca ccgccaccag cgcgtttact gtactggcgt gatttcagtc
     4201 ataaaaaaac ccgctcgcgg cgggtttaag ctgtgtggcg aagtaaccac tcttaacata
     4261 ctgacatact ttttgcggac cgcactaatc attttttact tttttagcag ccagtcgtcc
     4321 atctccagtc ttacccccag cacagacaaa catccgtcaa taaacccttc ggctatctgc
     4381 atctcaattc gtattgcttt ttcgcttttc tttctcgtcc tggctatctg tctttttgat
     4441 attcgcaaca aataatgagc aatgagaagc gaatactcct caggtttttt ctgcttcaga
     4501 cgagcaagac agttttcaat gataagtccg tcatcatcgc agcaggccgg acgtggttta
     4561 gtggcagatg gtaaaagtcc tttgaatccg gcagcgatcg gagaatagtc caccccggtg
     4621 ttaccacttg ccgcccatgc cccccagcgt tcaagaacca tctgaatatc acgcatcaac
     4681 tttctccaca aaatcaggac agcacaccaa tcgccagtgc gcgatcgata aaacgaaata
     4741 tcagctccag ttgggaacca tacttctctt caaatgccac agtatccgca tgcagttcgt
     4801 cgtggtgttt tctgcacaaa ggcagcacaa aaaggtcatg cgcttttgtc cccataccac
     4861 cctgaccgtg accaatcaga tgatgcggat catcggctgg ctttccacaa cacgcgcacg
     4921 gctgcgtctt aacccagcga gtgtactttt cattaaccca gcgacgacgt ttcgggcgta
     4981 acataaaaga ctccggcgac tccggatcca ctttcagcgc cagcaccttt ttcgctttat
     5041 cccggatgat gctggtggcg ggaaccgaag gcacaaggtc actttcccgg gtgacagacg
     5101 gcacaacagg cttcggtaat ctcagtgcct tacgggctgc actttccggt aaggcatccg
     5161 ccaggtcatt acgaaccagc caccagcaca gttccggcat tgtcacaacg tgactgtcat
     5221 caaaaccaag atcacggcgc acaacagaca acacccagcg ggcacagtta tccgttgcca
     5281 ttgcttccag ccgttccgtg aactggtcac ggagaagatt gtcacagtgc cagcacagac
     5341 ggattgcacc cggcgcgagc cgcattgtgg tcatgttctc gctgtgccat ccggaatgag
     5401 gccactgaca gcccttttca cgaagtaacc agctttcaag acattccacc ccaccagcac
     5461 gacgaataac tgcctcattg cggaacacgg cccgcacagc aggatcatcc gctaacggtt
     5521 gtgatgccgc cggaacggca ccactggcaa aagatgaata acgttccggc tcaggctcca
     5581 gcaggacacg cccctgcata aacaggggca tcagctctga gcctggtctg aacagtacga
     5641 tccccattcg cggggcaatt tcaggagtca acagtgctct cacggtcacc tcagcgaacg
     5701 atattgcatg aacacaggag aaaaaattca gccatcacgc agtaaactcc ttcaccagcg
     5761 tttcaaactg gcttaccttg ccttccagtt ccgccacgca atccaccagc tcatccactg
     5821 ccttttgtgt gcggtgtttt gcctgcagca gatcacgaag cgccggagta agctgcttgc
     5881 ggagcgtatc ctttgccacg ctcatttttt tccatctgtt cagcacaacg aagcatctcc
     5941 tgcgcctggc gacgaagttg ttccggtgaa acagtggctg ttctgttatt caaaataaac
     6001 gctccgcttt actgcccgac atgcggttat tgctgtatct gcgcggattg cccggcgtca
     6061 tgggtgtgga aagaacccgg gcactctcct ggtccacagg cagaaaatgc ccgttatgaa
     6121 aacgccggta aatggtaccc tgcgtgccat tacgctgttt cgtgatgttg atttctgcta
     6181 tgcctctcgc ctgtgtctcc gggttgtaca cctcatccct gtaaagcatc agaatgatgt
     6241 ctgcatccgc ctctatttcc ccggagtttt tcaggtccga gttcatgggg cgtttattgg
     6301 gtctggattc cacaccgcgg gagagctggc tcagcgctat cagcgggaaa ccgccggatt
     6361 ttgccaggct tttaagtccc tttgagattt cccccacagc aaggtcgtga cgccccgtac
     6421 tacgggtttt aatcaggccg aggtaatcga ccaccaccag cgccgtttcc gggtgtttca
     6481 tccggtggtg cctcgtggtt gcacatatct catcaatggt caggtttgcc tggtccacca
     6541 tccagatatg acgccccgtc attcgtccca cgccctgtga gaaacgcgcc cagtcttcat
     6601 cttcaaaacg ggcaacagac ttaagacggg ataccggcat tcccccggca gcagacacca
     6661 tacgttcacc aatctggatg ttcgccatct ccatggtgaa cagaagcacg ccatgcccct
     6721 gctcagtcac cttgtcgatg atatccagcg caagttcggt tttccccatc gaaggacgag
     6781 ccgcaatgaa taccaggtcg cctggctcca taccgcccgt ttttgcgtcc agttcatcaa
     6841 taccggtcat cagtgccctg gatttctcca gtccctgatt gcggcattca acacggtcga
     6901 ccacttccgg aaggacatca tcaatgtgaa ccggctgaat gacgcccttt ccggtcgaca
     6961 gtgaggtcat catgttctgc gcatccttca gggcatcctc agctgcttca caggtatgcg
     7021 catcacgtaa attctgtaat gcttcggtca gtgttttttc tgcatcgcgc agtgcggcat
     7081 tgcgccgcag cgctgcaaca tagtgctcca gtgaagactt cgcccaggtt ttgcgtccgg
     7141 tgtcggtaat caccggggca agttccggca tctcattgca cagcagtacg gggtcaatga
     7201 cgccggatac acgggcctgt ctgcaaattc ccgcgtaaat atccctgtac tgacgcacga
     7261 aaaagacatc cgccggaagt gtggccagaa tatccatcac ttccggatca gccccacgca
     7321 gaaaaaacgc gccaatgaca gctccttcca ggtcatcatt acgccacgcc ggatttgtcg
     7381 ggtttgtcat gctgccacac ctctgatatg cgcacggtag ctttcccaac caaacaccag
     7441 gcagttacgc ccaccatcag taacgcgatc cacaatccgt tcaccaatgg attccttaag
     7501 ctgttcaaac gtcaggttgc tgatcaaaat tgtcggtaaa acgctttcgt aacgcgcatt
     7561 tatgatttcc tgcaggatgg ttatctctgt aggcgtgccg aactgcacac caacctcatc
     7621 gatgataagc agatccagcg atgcaaaatg attaatcact tcatcgtcag tgcgctcaga
     7681 gttgtggcgc caggtatttt tcaccgcacg ggtaagccgc atcacatccg tgatttctat
     7741 ggttgcctgg tggtggcgaa taatattttt tgccattgat acagccaggt gatttttccc
     7801 ggtaccacaa ttgccaatca tgaccatgct ggtgcctgca gcgagacatt tcttccagga
     7861 agcggcatag cgctgacagg cttcaaggtt tttctgtgcg tcagtattca ccgcctgata
     7921 attctcaaac tcacagtcct ggaaccggcg ggcaataccg gcctgatcga gtaattcacg
     7981 aaccttcagg gcgcgtaatt cgtcatacac gcggtccagc tcctcactga ggcaggacag
     8041 gcaaccggac acccgtttga cagctttccc ccttacatcc gggcccgtca gcacatagcg
     8101 cgtgtatttt ccgtgtttcc cgcaggacac cgtctcagtg ctttgcaccc aatgctcaca
     8161 gcgccacgga cgctttccgc cacggacaaa tgccagctcc tcttccaggt ctgccttgcg
     8221 gttaagtaac tgcgctttgt cgtgttgcat gtgttgtttg ttcaggaaat tagtcatttt
     8281 caccctcccc acaaaatcac cagttgaagt tcgttgagcc gtagtcctgt tcactgaatc
     8341 ccgagatcgg gaggcttttg ccccgcccac ctccgggggc tgctggctgt tgccaggatt
     8401 cctcgaaatg acgatcgggt ccaaagaacg tcgacgcctg cttcacgaac tgggtaccgg
     8461 tatttccaga gacacgcacc caggcggcat agcgtttcac accgttgagc atggtttcgg
     8521 gtgtcacacc ttccctgatt cgggctttcc aggctttgaa ggctgctgac ttggaattac
     8581 caccagcacg tttgggatat tcctgccagg cctgttcaaa ttccggtgaa tattcctgtc
     8641 tggcagaacg cgctggtgca gacgcgtcag cggatgcgcc aatatcttgc ggttcatgtt
     8701 ttgaatttac tggtggttca tgttttaaac cttgtggatc tggggtcaga ttctgaaggg
     8761 tcaaacgcgt atttttgcca gaatctgaag ggtcaaacac acctgaacat acagattctg
     8821 acggttcaga ttttgaaggt tcagaatctg aagggtcacg taatcctgaa agtctgcgct
     8881 gctgtttcag ttctgcaacc ttatcccgct cagttctggc aagcagctca agccgatcag
     8941 cattcagatg ataaagattg gacgtattac ggttaccttt gcggcgtgac tgacgcgtca
     9001 gccagccatc agcctccagt tcggagattg ccgttctgac tgtactttct cccaacccaa
     9061 gctgtcggca tatggtttca acacctgggt agcacacccc gtcatcattc gaataatccg
     9121 ccaagcgcgc cataatcatc agttttgcac ctttgatgcc atatgctgca catgcatccc
     9181 agacattacc taggattttg ctactcatac ggcacctccc agacgcttaa acatttttcc
     9241 ggactgaaac gccaccagcg gataactcag ggtatgagta cgtccctgaa cctggcagac
     9301 aaccttctgg ctttctatat tgaccaggca aacccgcaga acgtgtccgt tgctggtggt
     9361 gaaccactgc cccacacggg ggcaatggtt gtatcggtga tacaggaaat taacgatgtg
     9421 gcggatcatg gacgcacctc cttgtcagaa ccattcagcc tggaatcaac aagtgcagca
     9481 ccaaaaacag catcacctac acggtcgtac agtttgctgg ccagcggaga ttcaacagcc
     9541 ttaagcattg gataaagctg gcttgtccag atttgatgga tttcacgcaa atgcaggtat
     9601 acgcctctgg cgtttcgtgc gacagctgac atatcagacg catcggcacc tgataaactc
     9661 ttctccatct ggttaaaggc attgatgtat gcctctttga accgggcagc acgtttacca
     9721 gtgaagccca tagcaagaaa cgcaaagccg tcgcgggtta tttgatagca aggaagtttg
     9781 cgagtaccgc cgttgggctg gcgtaccaaa attgatgtct ccgcaaaatt gcgggcacaa
     9841 aactctggag aacaatccaa aatgcggatc tttttcagaa catcgtcatg acgtttagag
     9901 aagaagtcag caacagccaa agaagatgta acagcctgac catcaacgat ggcaatttca
     9961 ggttgagaga gggttgggag agtagtcata gtgacagccc cggtagtcag ttttttagaa
    10021 aactcaccac atgggacgcc aatcacagag gtggtgagac gtacagggtt ggcgttaccg
    10081 gagactaccg aacccggccc gaccgaagtc ggccctgtac gccccaccat aatttgggcg
    10141 tagtaatgct catgacacga aaaaaccgca tgagcgcggt tatgctcagt aatcaatttc
    10201 aggacgccaa tcccggcacc cgttttataa ggtgccggaa cagtgtaacg tcccggactt
    10261 gcagaatcaa tatgctggtg gcgcgtcatt gatgcacctc ccgttgatta cgacggaaca
    10321 cggagtgacc ctggacggct tcagcctcat ggaatgcttc aatgcagctc tcgtagtacc
    10381 gcatggtgcg cagacttaac ccaagctgta gcatcatcag accatcacgg gtgatgtaat
    10441 aaccacgcac tgaatcgcca taggcgcgat aagtgccggg aataaagttg cgggtgagaa
    10501 attcgcgtga gcagttgaga tattcgattc gatcgatgat attcttatgc atgcgcttaa
    10561 agtgacaggc gacatacagg gagaatataa cggctctgcc gttgacgacc tcgattttga
    10621 ggtatggatg tgcggggatc gtagccatta aggcagcctc ctgtaacagt ggaacagttc
    10681 caccaccgga ggtctcaatc tcgctggtgg cggactggac agggttgaga ctaccggcgt
    10741 tacaggaaac cggcgaacct ttcggttccc ctgcccagcc caccataatt ctggcgtgca
    10801 aggacgctga caacaaaaaa gacgctggcg cgtcatttgt cgcctgtaac aattccgggg
    10861 tctcactccc gatagtggat tttgccacta tatgtaaagt gtgccgccgg aatttaattt
    10921 tgtcaacgag gttaaatata gagtcgctat gttggtggcg cttcgcactc aacaaaatca
    10981 cgacgaaaca accacagcgg gatgaagcat tcatgcggat aaccatcacg caggtaaata
    11041 acccgctgtg tttcagactc ccagcgtata acgtggacgc gacgccccct tccatcacgg
    11101 aaccagcgat tgagtacttg catgtgttac ctgtgagcat aattacacct gccagcccag
    11161 cgcctggaac aaccccattt tcgggtgata ccagcgagta cctcgtggtt ctgcttcact
    11221 cgtcatgcga tgaaaagcag acatgaaggg ttccacagca acaatagcgc gacgcgataa
    11281 caatccatcc ggcgtcatga actcatgggt gtcggtaggg atctgatatg cgttcaccag
    11341 attgcggcat ttcgcatctg acatacccgt tttcgccgcc agctgacgat agcctgcata
    11401 accatcgcgt atggcgcctc ttttgatttg ttcgacagtt tcggtaacgt gactgacttt
    11461 ttcttctacc cgctcaaggc gtttctgctg acgaacagct tcaagagcca tcgcggcaac
    11521 catttcgatt tcgctcattg gcttgcggat ctgttcttcc agttcgcgcc agcggtctac
    11581 cagacgggct gtgaattcag ggcaaagctg tgcgacaaca atgatgctgt cgcgtttgcc
    11641 ttgttcgcct tcaaacagat aatgctcgtg ttgaacttta aaccctaagt tattgattct
    11701 ttcggaaacc tcaatttgag gagatcggac aacaccacct ttagctaatg tttcaatagt
    11761 gcgtttcaca ttgtcatgac gcttacccac cagctctgcg atctcaacgc tggtcatgga
    11821 tgctttatcg gtaaaaattg ttgtattcac tgtttatctc cttcgcacac tccatcttct
    11881 gtgtgcgcta aacttgggtg tgggaaaagc tccggtagat caggacgaaa ttcatacgcc
    11941 tggactttcc catcgaccgc cttaaccaga gacggaacaa attcaggaga aatttttttc
    12001 ttcccattca gccagtcgca aattgttgac tgcgcgcgac cacaacgcct agccagagca
    12061 atctggcttc ccgcaatatc tacagccttg gctatacctt catttttcat atcgacctcc
    12121 aacctgattc cattcactat agcgattgat taaagcgatt gcaatgtacg ggaaagcggt
    12181 ttgtgtatat atcgctaagg cgatacacta aaagggcaga aaaaggagga cttatgagct
    12241 tttcagacag gctacagtta gctatgacta tccgcggata ctcgcaggga aaactggcca
    12301 gagaggttgg catggctcaa tcgagcgtaa ataaactggt taatggtgcc acaggctcca
    12361 ggaaagttgt ggagatagcc aataccctga acgtgcgccc cgagtggcta tcgtacggtg
    12421 ttggtcctat gaccccagaa gaaagcacat cctgcaatcc gacaccacag gtatcatatt
    12481 ccccgctgga aaatgataag gatatttttc gtgtggaagt cctggatatc gctgccagcg
    12541 cgggaccagg aacattcctt gtttcagatt ttgcagaaac ggtccatgct atagaatttt
    12601 cacatgacgc cgcaagacgt ctgttctgta gcaggccagc aaatatcata aagatgataa
    12661 cagttgatgg cgacagcatg gctcctactc tgtgtgcagg agatcaggtg tttgtcgacg
    12721 tatccgtcag aaattttgaa actgacggta tctatatttt tatttttggg cacactttcc
    12781 atatcaaacg cctgcaaatg caagggatgc aacttgctgt tatatcagac aatccggcct
    12841 acaaggaatg gtttataagt gaatcagctg aagaacatct attcataatg gggaaagttc
    12901 tcattcatca gtcaatccag tataacaggg tcggataagt caacatcacc ggcagctgta
    12961 ttgcgcagct gctctacttt cctgtatctc aactccctac gtatcgtctc atttctctca
    13021 taatctgttc tttgctgccc aaaatgcata tctatagcga ttgacataac caaacgcttt
    13081 ggctattgta aaattaaaac aacaggaggc tcatatgaag catgcagtta aggtgatgga
    13141 tgaaattcag cacaaataca agaaagcggc tcgctgcttg gaatgatcta ttcctacatg
    13201 tcagaaaacg gagcagaaag tgagagggtt tataatgcaa tcagatgcct gatgcgttcc
    13261 ctgcaaaaaa cagaagaa
//
LOCUS       DAZGBC010000138        13273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGBC010000138 DAZGBC010000000
VERSION     DAZGBC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.139"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..162
                     /locus_tag="ACHK1J_003837"
     CDS             22..162
                     /locus_tag="ACHK1J_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7913047.1"
                     /translation="MRQAVAVMPHAAKVLKQVMVNTGGNKRQVPAKRQNRGSRNSTDD
                     GG"
     gene            186..488
                     /locus_tag="ACHK1J_003838"
     CDS             186..488
                     /locus_tag="ACHK1J_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913048.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            644..931
                     /locus_tag="ACHK1J_003839"
     CDS             644..931
                     /locus_tag="ACHK1J_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913049.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
     gene            1050..1871
                     /locus_tag="ACHK1J_003840"
     CDS             1050..1871
                     /locus_tag="ACHK1J_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7913050.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVPGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKKNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRGITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(2168..2758)
                     /locus_tag="ACHK1J_003841"
     CDS             complement(2168..2758)
                     /locus_tag="ACHK1J_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7913051.1"
                     /translation="MVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFINVTC
                     KAGDCFDLAGRDYKIDPDLLRAISWKESRNRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVFWEAVGAYNAGFRKTERQNQRRLA
                     YASDVYRIYSGIKNSKGIQLRASEKPLSKMNSSQKN"
     gene            3091..3474
                     /gene="traM"
                     /locus_tag="ACHK1J_003842"
     CDS             3091..3474
                     /gene="traM"
                     /locus_tag="ACHK1J_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO7913052.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            3668..4315
                     /locus_tag="ACHK1J_003843"
     CDS             3668..4315
                     /locus_tag="ACHK1J_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFO7913053.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHSIKCQNCDSEIWFSGIDIDTQILFQTAEVDAFSSSNGVSIINGVILNDVSWDVVIE
                     STEVNDLNLFVWRFFERYVVSSFFIKGKEFCCESDKYRFNKIIPYLLGYSHEYSAQRM
                     DVSIDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINNLHRKEM"
     gene            4434..4661
                     /gene="traY"
                     /locus_tag="ACHK1J_003844"
     CDS             4434..4661
                     /gene="traY"
                     /locus_tag="ACHK1J_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO7913054.1"
                     /translation="MIKGALRAAPSRKVLLTLDEATNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVTEERESTFKEL"
     gene            4696..5049
                     /gene="traA"
                     /locus_tag="ACHK1J_003845"
     CDS             4696..5049
                     /gene="traA"
                     /locus_tag="ACHK1J_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO7913055.1"
                     /translation="MNTSVSVPGAFTPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            5064..5375
                     /gene="traL"
                     /locus_tag="ACHK1J_003846"
     CDS             5064..5375
                     /gene="traL"
                     /locus_tag="ACHK1J_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO7913056.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            5397..5963
                     /gene="traE"
                     /locus_tag="ACHK1J_003847"
     CDS             5397..5963
                     /gene="traE"
                     /locus_tag="ACHK1J_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO7913057.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            5950..6678
                     /gene="traK"
                     /locus_tag="ACHK1J_003848"
     CDS             5950..6678
                     /gene="traK"
                     /locus_tag="ACHK1J_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO7913058.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGQLPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLVAARMDVYVIRDGEGN"
     gene            6678..8108
                     /gene="traB"
                     /locus_tag="ACHK1J_003849"
     CDS             6678..8108
                     /gene="traB"
                     /locus_tag="ACHK1J_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO7913059.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVLNKQRGDD
                     QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPEGEPQPGNAPVAFPP
                     QGAGAVPPPTAFYPGNGATPPPQVTYQSVPVPNRIQRKTFSYDAGTKQTPSLPYIPSG
                     SFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQVEESNPGSPVPNPPSAESHLNGLNTD
                     QMLKQLGDLNPQQFMAGGQEGGNDGK"
     gene            8098..8682
                     /gene="traP"
                     /locus_tag="ACHK1J_003850"
     CDS             8098..8682
                     /gene="traP"
                     /locus_tag="ACHK1J_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO7913060.1"
                     /translation="MANNTSSSQACHTARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFIRQTAPAGQFPVRECLPSLPDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYLVRPGKMKCIRVVTADEHLKEV
                     YTEDASLPGKIRKCHAYFPDNRTNRNNGDKNEHA"
     gene            8669..8863
                     /locus_tag="ACHK1J_003851"
     CDS             8669..8863
                     /locus_tag="ACHK1J_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO7913061.1"
                     /translation="MNMRNIHVITALSVPDKRISDDFMHAVLSNCTTGVLPAPEKFGS
                     ESLPDNFTMTAVGVMKNSEI"
     gene            8875..9126
                     /gene="trbG"
                     /locus_tag="ACHK1J_003852"
     CDS             8875..9126
                     /gene="trbG"
                     /locus_tag="ACHK1J_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO7913062.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            9123..9638
                     /gene="traV"
                     /locus_tag="ACHK1J_003853"
     CDS             9123..9638
                     /gene="traV"
                     /locus_tag="ACHK1J_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO7913063.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLQRLAEGNFRTMPVQTVTATSPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVIPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            9773..9994
                     /gene="traR"
                     /locus_tag="ACHK1J_003854"
     CDS             9773..9994
                     /gene="traR"
                     /locus_tag="ACHK1J_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO7913064.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            9987..10460
                     /locus_tag="ACHK1J_003855"
     CDS             9987..10460
                     /locus_tag="ACHK1J_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913065.1"
                     /translation="MHKSENEKIDSILDEHQQIYRRHNRIDNILAFFACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            10540..10758
                     /locus_tag="ACHK1J_003856"
     CDS             10540..10758
                     /locus_tag="ACHK1J_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913066.1"
                     /translation="MHTFIFLVLMKNVGGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGL"
     gene            10786..11133
                     /locus_tag="ACHK1J_003857"
     CDS             10786..11133
                     /locus_tag="ACHK1J_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913067.1"
                     /translation="MKSIYLKYFLSFVFVGVMAMIVCIPFYIIYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTFGKANKIASECRKRNEMEEARRVRENELNKIREKQ
                     LQALSDADSAKER"
     gene            11264..>13273
                     /gene="traC"
                     /locus_tag="ACHK1J_003858"
     CDS             11264..>13273
                     /gene="traC"
                     /locus_tag="ACHK1J_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO7913068.1"
                     /translation="MSNNPLEVVTQAVNSLLTALKLPDESAQANQILGEMNFPQFSRL
                     LPYRDYNQESGLFMNDSTMGFMLEAIPINGANETIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGERIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGLNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVA"
BASE COUNT         3516 a         2904 c         3442 g         3411 t
ORIGIN      
        1 tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag tgatgcccca tgcggcgaag
       61 gttctcaaac aggtgatggt gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt
      121 caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaacatca gatacaggag
      181 gaaacatgag cgcattttct tttaacaccg cttatcagcc aacaggcgat cagcagaaag
      241 acgcaatcgc acaaatcgac atcatgcaga atcgtgctgt tcaggcaaat cttgcatacc
      301 agagcagcct ggatgcggaa acgctattga aacagttatg gcaaatcaat gatgaactgg
      361 gggaactgtt ggatagcgtg gaaaatcaca cgccagttat cagaaaaaaa gccagtgatt
      421 tgagcgcact gatgatgctt ttcagccaac aggccggtca gcctatgact atgttgttga
      481 tttgttaatg ataatcaggg gcaggaaatt aagcgaaaga tgctctgccc cgggtttgca
      541 gcgaccggaa cggtcacgcc acccttgtcg cgctgtggcc tgcggcggct cccgaaactc
      601 aaccgatgat gggggctgaa aaccagagag aggagaaagc aggatgagta cccgaaatat
      661 ccacgttaac accgcgtcgt acacgcttct tgttgccggg aagaaaaaaa acaccgggga
      721 agagtgggac gtcctggaat tcagcagcct gactgagctg aaaaaatatc gcaaaagcca
      781 cccggaaaag atggccttca gctatagcta cgcgctcagc cggggtgtgg atacgcagtt
      841 ccgccatatc aacattgcgg aagccgatca cttcaaacag ttcctgcgtc agattaagcg
      901 taccggcctc gatatccggg cgatctgctg accggatgtg atgaatatcc ctcctgattg
      961 ttgcaatctt gttaatgggg accgggaggg atgatattaa gcggaaaaat tttttattaa
     1021 atttattctt tcataaagga gtagctgtta tgcgattagc ttcccgtttt ggtcggtata
     1081 attccattcg ccgtgagcgt cctttaacgg atgatgaatt aatgcagttt gtgccttcgg
     1141 tattttccgg tgataaacat gaatcccgga gtgagcgtta tacatatatt ccaacaatca
     1201 atatcatcaa taagttacgt gaagaaggtt tccagccgtt ctttgcatgt cagagtcggg
     1261 ttcgggattt gggacgccgc gaatacagta aacatatgtt acgtcttcgc agggaagggc
     1321 atattaacgg gcaggaagtt cctgaaatta tcctgcttaa ttcacatgat ggttcatcca
     1381 gttatcagat gatcccagga atttttcgtt ttgtctgcac aaatggtctg gtgtgcggaa
     1441 ataattttgg cgaaatccgc gttccacata aaggtgatat tgtcgggcag gttatcgagg
     1501 gtgcgtatga agtgcccggt gtctttgata aggtcactga taatatggag gcgatgaaag
     1561 aaatacatct taacagtgac gagcaacatt tatttggcag agctgcactg atggtcaggt
     1621 atgaagatga aaataaaaca ccggtgacgc ctgaacaaat aattactccc cgtcgttggg
     1681 aggataaaaa gaacgatctc tggacaacct ggcagcgggt tcaggagaat atgataaaag
     1741 gtggactgtc ggggcgaagt gcctccggta aaaataccag gacaagaggc attacgggta
     1801 ttgatggtga tatccgaatc aacaaagcat tatggatgat tgccgaacag ttcagggagt
     1861 ggaagtcatg ataatataag taaagccccg aaaatttttt cggggcttta cttatattat
     1921 cattatgcgg cattgatatg tcgtctgaga tgcctcaatt tcatcaactg gcttttcggt
     1981 agtctgtaaa tattgtgccc gcgaaaaatg gtctggcagg aggctgcgca gttaccggat
     2041 atatataaga tgggttcccg ctttgcgggg cttcggccag tcagcattat cactcatatg
     2101 atatttttgt gtgtggcctt ccatgccgcg tttgcggcat aaccggtttt ttaaaatgta
     2161 tatcaaatta attcttctgc gagctgttca tttttgaaag cggtttttca gatgcacgaa
     2221 gctggatccc cttgctgttt tttatcccgc tgtaaatccg gtaaacatct gatgcatagg
     2281 caagacgccg ctggttctgg cgttcggttt tcctgaatcc tgcgttgtat gcaccaacag
     2341 cctcccagaa gactccccat tttttaaagg ctatagccag ataataagca ccggtataaa
     2401 tgttcagaca gggatctgct gtcagatgtt ccggtttaat tccgtagcgg gccagttcgt
     2461 taaaatgctg ggaatctacc tgcataagtc cactgccata tcccgtaacc ggattaatac
     2521 cgatggcatt aacccggtta cgggattctt tccatgatat tgctctcagc aagtcagggt
     2581 caattttata atctctgcca gccagatcaa agcaatcacc agccttgcag gtcacattta
     2641 taaacatcag gcagatcgtc agaagccatt ttctcatttt ctcacctctg gtgacgtcat
     2701 caggaaataa tttaacccac tccacaaaaa ggctcaacag gttggtggtt ctcaccacca
     2761 aaagcaccac acactacgca aaaacaagtt tttgctgatt ttcgtttaat atcattgagt
     2821 tgtatttgcg gatttatatt gtttaaatct gatttattta aaacagcgtc gttaacgccg
     2881 ctacagcaac gcgcagacac cgctttttag gggtggtact gactattttt ataaaaaaca
     2941 ttattttata ttaggggtgc tgctagcggc gcggtgtatt tttttatagg ataccgccag
     3001 gggcgctgct agcggtgcgt ccctgtttgc attatgaact ctggtgtttc taaattaact
     3061 ttattttatg ttcaaaaaag gtaatttcta atggctaagg tgaacctgta tatcagcaat
     3121 gatgcttatg aaaaaataaa tgcgattatt gagaagcgtc gacaggaagg ggcaagagaa
     3181 aaagatgtca gtttttcagc aacagcttca atgcttcttg aactggggct tcgtgtatat
     3241 gaagctcaga tggagcgtaa agagtccgcg tttaatcaga ttgagtttaa taaattactt
     3301 cttgaatgcg ttgtaaaaac acaatcatca gtagcgaaaa ttttgggtat tgagtctctc
     3361 agtcctcatg tttccgggaa tccaaagttt gaatatgcca atatggttga agatatccgg
     3421 gagaaggtgt catctgagat ggaacgattt tttccaaaaa atgatgagga ataaaaaaga
     3481 attaaaagtt gattttattc aaataacaga atattgatct agttctattt gatgacaatt
     3541 aaccacataa cgagtatatt aattatgtgg ttaatgccac gttaaaattt gaacttgaaa
     3601 atcgccgatg cagggaggga ctagctccct gcatcgactg tccatagaat cctttgagga
     3661 ggttcctatg tgtgcgatgg atactagaga aagagcactc atacgtcaat caggcctgcc
     3721 ggaaattatt ttatccgcaa aatatccagt ctgtgtccgc acagagtcag gaaaattcat
     3781 agactcaaac tctgtttttt tacactcaat aaagtgtcag aattgtgatt ctgaaatatg
     3841 gttttctggt attgatattg atacgcaaat tttatttcag actgctgaag ttgatgcttt
     3901 ttcgtcctca aatggagtga gcattattaa tggtgtaata ttgaatgatg tatcatggga
     3961 tgtggtgatt gaaagtaccg aagtgaatga cttgaatttg tttgtctgga gatttttcga
     4021 aagatacgta gttagtagct tttttataaa gggcaaggag ttttgttgtg aatctgacaa
     4081 atatcgcttt aataagataa taccgtatct tttgggttat agtcatgagt attctgctca
     4141 gagaatggat gtctctattg accagtctaa aaaaatattt atgaatttta aaaaacacta
     4201 tggtttctca gacagggatg agtggttgcg atatgctata atttctgatg tgttatttta
     4261 tctatatcaa tacttcgcta gtaatattaa caaccttcac cgcaaagaaa tgtaagtatt
     4321 cccggcacta aaagtgccgg tttttgttaa cgtaatttac tcttttgttg tttgttttgt
     4381 gtgcgtctta tgtaaatatt ttgactaaat ctcttttatg tacgggaggt gtggtgatca
     4441 aaggtgcttt aagagctgct ccaagtagaa aggttttact gacccttgat gaggcaacca
     4501 ataaattatt acttggagcc agagagcgca gtggaagaac gaaaacaaat gaagcattgg
     4561 tgaggttgca ggatcatttg caacgttatc cagattttta taacgaagag atattcaggg
     4621 aggtgacaga agagagagaa tcaactttta aagaacttta gttttaatct ttttacatac
     4681 aatccgaggt aattgatgaa tacttctgtt agtgttccgg gggcttttac gcctgttaaa
     4741 aagaaatcat cttttttctc taaactgaac ctgcttcgcc tggttcgtgc tgtgatcccg
     4801 gcagctgtgc tgatgatgtt cttccctcag atggccctgg ctgctggcgg tcaggacctc
     4861 atgcagtccg ggaatgacac agtcaaagcc acctttggta aggactccag tattgttaaa
     4921 tgggtcgttc tggctgaagt gctggtcggg gcggtcatgt acatgatgac caaaaacgtc
     4981 aagttcctgg ccggttttgc catcatctct gtatttattg ctgtaggtat gaccgtcgtt
     5041 ggcctctgac aggaaataaa acgatgtcgg gagacgagaa taaacttaag aaatatcgtt
     5101 tcccggaaac actgaccaac cagagccgct ggtttggcct gccactggat gaactgatcc
     5161 ccgcagcgat ctgtattggc tggggtatca caacatcgaa atatctgttc ggtattggtg
     5221 cggcggttct ggtttatttc gggataaaaa agctgaaaaa agggcggggc agcttctggt
     5281 tacgggatct tatttactgg tatatgccaa cagtcctgct gcgcggtatt tttcataatg
     5341 ttcctgattc gtgtttccgg cagtggatta aatagaactg atacctggat tgttatatgg
     5401 aacacggtgc ccgcttaagt accagtcgtg tactggccat tgcttttata tttatgtccc
     5461 tgctcattgt tctcagcctg actgttaacg tgattcaggg ggtgaataat taccgccttc
     5521 agaatgaaca gcgtactgcc gtgacaccga tgggatttaa tatgcccttt gcggtatcac
     5581 agaacagtgc tgatgcctct tttttgcagc agatggcgct ggcgtttatt gccctgcgcc
     5641 tgaatgtctc accggaaatc gtcgatgctt ctcaccaggc acttctgcag tatatccgtc
     5701 cgggcgcgca gaataagatg aaagtgattc tggccgaaga agcaaagctc atcaaaaaag
     5761 ataatgtgaa ttctgctttt ttccagacca gtgttcgtgt ctggccacaa tatggtcgtg
     5821 tggaaatccg tgggattcgg aaaacatgga ttggtaactc cgaacctttc actgatatca
     5881 aacattacat tcttattctg aaacgggaaa acggggtgac ctggctggat aatttcgggg
     5941 aaacagacga tgagaaaaaa taagacagta ataatatccg cctgcctgct tttttccggc
     6001 agcgtgctgg caggaaacgg cacactggca cctacagtgg ttccgatggt caatggcggt
     6061 caggcaagta ttgccatcag taataccagc ccgaatctgt ttaccgtccc cggtgaccgg
     6121 attatcgccg tgaacagtct ggatggtgcc ctgaccaata atgagcagac tgcgtccggt
     6181 ggtgtggtgg tggccaccgt caacaaaaag ccttttacgt tcattctgga aacagaacgt
     6241 ggtcttaatc tttccattca ggccgttccc cgtgaaggcg ccgggcgtac cattcagctg
     6301 gtcagcgatt tgcgcggaac aggagaaact gccggtgcat gggaaacatc cacgccttac
     6361 gaatccctgc tggtttccat cagtcaggcg gttcgtgccg gacaactgcc agaaggctgg
     6421 tatcagatcc ccgtgacaaa ggaaaccctg caggcccctg ccggaatgtc cgcaaaagct
     6481 gaagccgtat ggacggggaa tcacctgaag atggtgcgct ttaccgtgga aaataaaacg
     6541 ctgtctgcgc tgaatatccg ggaaagtgac ttctggcagc cgggaacccg ggcggtgatg
     6601 ttcagccagc ctgtcagcca gttactggtc gctgcccgta tggatgtgta cgtcattcgt
     6661 gacggggagg gcaactgatg gccagtatca acaccattgt gaaacgcaag cagttcctgt
     6721 ggctggggat tgtgattgtc ggatcggtat cagcaattgg tggtgcgctt tatctgtctg
     6781 atatggatat gtccgggaac actgatgccg ttgcagggca ggagcctgtg ccggatatga
     6841 ccggtgttgt ggatacgacc tttgatgaca aggtgcgtca gcatgccacc acagagatgc
     6901 aggtgacggc ggcacagatg cagaagcagt atgaggaaat ccgtcgtgag atggatgttc
     6961 tgaacaaaca gcgtggtgat gaccagcgtc gcattgaaaa gctggggcag gagaatgctg
     7021 cgctggcaga gcaggtgaaa gccctgggcg cgaatcccgt tacagtgacc ggagagcctg
     7081 tgccacagat gccggcatcc cctcccggtc cggaggggga gcctcagccc ggtaatgcgc
     7141 ccgttgcttt tccaccgcag ggggcaggtg cagtgccacc gcccaccgcg ttctatccgg
     7201 gcaacggagc cacgccgccg ccacaggtga cgtaccagtc tgtgccggtg ccgaaccgga
     7261 tacagcgtaa aacgttcagc tatgatgctg gcacaaagca aactccgtcc ctgccgtaca
     7321 ttccgtcagg cagttttgcg aaagccattc tgattgaagg ggcggatgcc aatgcttctg
     7381 tcaccggtaa tgaatccacg gtgccgatgc agctgcgtat caccggtctg gtggaaatgc
     7441 cgaacagcaa aacctatgac gcaacgggat gctttgttgg gctggaagcc tggggggatg
     7501 tgtccagtga gcgtgccatc gtgcgtaccc gcaatatcag ctgcctgaag gagggtaaaa
     7561 ccatcgatat gccggttaaa gggcatgtca gcttccgggg taaaaacggt atcaagggtg
     7621 aagtggtgat gcgtaacggc aaaatcctcg gctgggcatg gggcgcggga tttgttgacg
     7681 gtatcggtca gggaatggag cgcgcttccc agccggctgt cgggctgggt gccactgccg
     7741 cttacggggc cggcgatgtc ctgaaaatgg gtatcggtgg cggtgcatcg aaagcggcac
     7801 agacactcag tgactactac atcaaacgcg ctgagcagta tcacccggtg atacccatcg
     7861 gggcgggtaa tgaagtgacg gtggtgttcc aggacggttt ccagctgaaa accgtggaag
     7921 agatggcgct ggagcaggct caggggcagg tggaagagag taacccggga agtcccgtgc
     7981 cgaatccgcc atccgcagag agtcacctga atggtctgaa taccgaccag atgctgaagc
     8041 aactgggcga cctgaatccg cagcagttta tggccggtgg tcaggaaggg ggcaacgatg
     8101 gcaaataata cgtcgtccag tcaggcgtgc cataccgccc ggtatgttgt cgcccgggtt
     8161 ctgcgtggac tgttctggtg tctgaaatac accgtcattc tgccactggc cacaatggcc
     8221 ctgatggcgt tatttgtttt atggaaggac aacaccacac cagggaaatt actggttcag
     8281 gaaattgact tcataagaca aacagcccct gccgggcagt ttcctgtaag agaatgtttg
     8341 ccttctctgc cagatcttcc aggagcagaa gagaaaatct gccgttaccg tgctgctgat
     8401 gctgcagatt atgtcaggga gactgaccgg tcactgatgc aactggtcac cgcattatgg
     8461 gcaacactgg cactgatgta tgtctcgctg gcagcaatca ccgggaaata tctggtgcga
     8521 cccggaaaaa tgaaatgtat ccgggtggta accgcagatg aacatctgaa ggaagtttat
     8581 acggaagacg cttccctgcc gggaaaaatc aggaaatgcc atgcttattt ccctgataac
     8641 agaacaaaca gaaacaacgg agataaaaat gaacatgcgt aatattcatg ttattacggc
     8701 cctctctgta ccggataaac gcatatcaga tgattttatg catgctgttc tcagtaactg
     8761 caccacagga gtacttcctg caccggagaa attcggttct gagtcattgc cggacaattt
     8821 tactatgact gctgtcggtg tgatgaagaa ttcagaaata taaggaaccc ttcgatgaat
     8881 aagctggttt ctgatggcag cgtcaaaaaa ataaattatc cggtgctgta tgagtccgga
     8941 ataacacctc cgttatgtga agtcagtgca ccagaacctg atgcgggtgg gaaacgcatt
     9001 gtggcatatg tttataaatc atcacggaat acggtttttg aaaatcctga cattgtaaaa
     9061 acctgtacgg tcagggattt gaagaatgat tttgtgaact gcgatgagaa aggtgaggga
     9121 caatgaaaca gatttcttta tttattccgc tgctggggac gttattactt tccggctgtg
     9181 ccggaacaaa ctccgacttt gagtgtgatg ccaccacatc cgatacctgt atgacgatgg
     9241 agcaggccaa tgagaaggcc aaaaaactgg agcagtcctc agaagcaaag ccggttgcgg
     9301 catcactgca gcgcctggct gaagggaact tccggacaat gccggtgcag accgtcaccg
     9361 cgacctctcc gtccggcagc agacctgcgg tgacggcact tccggagcag aaactgctgg
     9421 caccacgtcc gctgtttacc gctgcccggg aagtgaaaac ggttgttccg gtcagttcag
     9481 ttgcaccggt aatacctccc cgtccgctaa gaacgggtga gcagacggca gcattatgga
     9541 tagcgcctta tattgataat caggatgttt atcatcagcc atccagtgtg ttttttgtta
     9601 ttaaaccgtc tgcgtgggga aaaccacgta ttaattaaca ggccctgaag ttgtatatca
     9661 gccggcaatg ccggcgggct ttctgtattc aaaatctgcc tgtaaaacga gtgccctgtg
     9721 cgtgaaaagg gatgggcggt agcgtgtcag aacatgtcgg gaggaatatt ccgtgagtga
     9781 tgaagccgat gaagcatatt cagtaacaga acaactgacc atgacaggaa taaaccggat
     9841 acgccagaaa ataaatgttc atggtattcc tgtttatctc tgtgaagcat gcggaaatcc
     9901 tattccggaa gcccggcgga aaatatttcc cggtgtgacg ttgtgcgttg aatgtcaggc
     9961 gtatcaggaa aggcagagga aacactatgc ataaatcaga aaatgaaaaa atagattcta
    10021 ttctggatga acatcaacag atatatcgca gacataaccg aatagataac atcctggcgt
    10081 tttttgcgtg tattgcatgg attccctgta ttattgctgc gttcggcgtt gatggtatat
    10141 atctgaaaat cctgtttgcg gtccttgcct gctgtggtgc ggtatgcttt tttattttct
    10201 tcttcactct cctgccggaa tcactcatgg tcctcagccg tcagagcctg cttcagctta
    10261 tgcgtctgac tgaagacgta ccggatgcga gacaggaact gcttaaccgt ctgctgtccg
    10321 gtaaaaaact gactggtcgg gatgaaaagg atatccggcg tctctggcag gagaaagtgg
    10381 atgcaatgca ggagtctgcc acccgtcagc gtgaacagga taccatccgg aagtttactg
    10441 aagggaataa atccgaatga aaatctgctg tgttttataa cctcataagt taactgaagg
    10501 agtattcagt gattaatatt tcactggagg taataaagaa tgcatacctt tatttttctt
    10561 gtgctgatga aaaatgttgg cggtggcgga agctggaatg ttacccccat gccaaatacg
    10621 gacgtatgcg tgcaggttct tcggacaatc gaagcacatg gaggcagggg atttggaaat
    10681 acaggggagt gtattgaagt taaaactaat gaacctttga ataacgtgat ggaaacactg
    10741 aatggtggtg ggctatgagt gcttactgct tcgaaggaaa atgaaatgaa gtcgatatat
    10801 ttaaaatatt ttctgtcttt tgtatttgtt ggtgttatgg ctatgattgt atgtattcct
    10861 ttttacatca tttaccttgc gcaacaacct gcaacaccag aacaactgac agagatttta
    10921 caggaaactc cctgtgcagc agaagcattt caggagacac ttaattatca gtctgaacct
    10981 ttaacatttg gtaaggcaaa taaaatcgca tcagaatgca gaaaaagaaa tgaaatggaa
    11041 gaggcgcgac gggtaagaga aaacgagctc aataagatac gcgaaaaaca acttcaggca
    11101 ttaagtgatg ctgattcagc aaaggaacgt taattgtaat cctggtttcc tgttaaaagc
    11161 ctccatctct tatttgtgct gtggtttcag aaatggcact gcgtgatata tccacgccac
    11221 agactgatat tttccttttt taaacgtact aaggaatact catatgagta ataacccact
    11281 tgaggtcgtc actcaggcgg tcaattcact gctgaccgca cttaaattac cggatgaatc
    11341 tgctcaggct aatcagatcc tcggtgagat gaacttcccg cagttcagcc gtctgctgcc
    11401 ataccgtgat tacaaccagg aatccggtct gttcatgaat gactccacaa tgggctttat
    11461 gctggaagcc attcccatca atggtgcgaa tgagaccatt gtggaggctc tcgaccatat
    11521 gctgcgcacc aaactgccgc gcggtattcc gctgtgtatc catctgatgt ccagccagct
    11581 ggttggcgag cggattgagt acgggctgcg tgagttctcc tggtcaggtg aacaggcaga
    11641 acggtttaac gccattaccc gggcctatta catgaaagcc gcagaaacac tgtttccgct
    11701 gccggaaggg ttgaacctgc ctctgaccct gcgccattac cgggtgttta tctcgtactg
    11761 ttctccctcg aagaaaaaaa gccgggccga cattctggaa atggaaaacc tggtgaaaat
    11821 catccgggcg tctctgcagg gagcatatat caccacgcag acggtggatg cacaggcttt
    11881 catcgatatt gtcggggaga tgattaacca taacccggac tccctgtacc cgaaaagacg
    11941 tcagctggac ccgtattctg atttgaatta tcagtgcgtg gaggacagtt ttgacctgaa
    12001 ggtccgggca gattacctga cgctgggact gcgtgagaac ggcaggaaca gcactgcccg
    12061 catcctgaat ttccatctgg cccgtaaccc ggaaatcgcc ttcctgtgga acatggccga
    12121 caactacagc aacctgctga acccggaact gtccatctcc tgcccgttca ttctgacgct
    12181 gaccctggtg gtggaagacc aggtgaaaac ccacagcgaa gccaacctga agtacatgga
    12241 cctggagaaa aagtcgaaga cctcctatgc caaatggttt ccgtccgtgg agaaagaagc
    12301 gaaggagtgg ggggaactgc gtcagcggct gggctccggt cagtcttctg tcgtgtccta
    12361 cttcctcaac atcacagcat tctgcaagga caataatgaa acggcactgg aagtggagca
    12421 ggatatcctg aacagcttcc gtaaaaacgg ttttgaactg atttcaccgc gctttaacca
    12481 catgcgcaat ttcctgacct gcctgccctt tatggccggg aaagggttgt ttaaacaact
    12541 gaaagaggcc ggagtggttc agcgcgcaga gagctttaat gtggccaacc tgatgccgtt
    12601 agtggcggat aaccccctga cgccggcagg tctgctggca cccacctacc gtaaccagct
    12661 ggcgtttatc gatattttct tccgggggat gaataacacc aactacaaca tggcggtctg
    12721 tggcacttcc ggggccggta aaaccgggct gatacagcca cttatccgca gtgtgctgga
    12781 ctccgggggt tttgctgtgg tgttcgacat gggggatggc tacaagtccc tgtgtgaaaa
    12841 catggggggc gtatatctgg acggtgaaac cctgcgcttt aacccgtttg cgaacatcac
    12901 cgatattgac cagtcagcgg agcgtgtccg tgaccagctg tcggtgatgg ccagccccaa
    12961 cggtaacctg gatgaagtgc atgaaggtct gctgctgcag gcggtcaggg cctcctggct
    13021 taccagacag aaccgggcgc gtattgatga cgtggtggat ttcctgaaaa acgcccggga
    13081 taacgaccag tatgccgagt ccccgaccat ccgcagccgt ctggatgaaa tgatagtgct
    13141 gctggaccag tacacagcca acggtactta cggccagtat tttaactctg atgaaccgtc
    13201 cctgcgggat gacgcaaaaa tggtggtgct ggagctgggt ggactggaag accgtccgtc
    13261 cctgctggtt gcg
//
LOCUS       DAZGBC010000139        13190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGBC010000139 DAZGBC010000000
VERSION     DAZGBC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.140"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..216)
                     /locus_tag="ACHK1J_003859"
     CDS             complement(<1..216)
                     /locus_tag="ACHK1J_003859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913069.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEI"
     gene            153..1652
                     /gene="pitB"
                     /locus_tag="ACHK1J_003860"
     CDS             153..1652
                     /gene="pitB"
                     /locus_tag="ACHK1J_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO7913070.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHK1J_003861"
     CDS             complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHK1J_003861"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7913071.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVVKLPLMGKSLNKSIERNKVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACMRKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2567..3280
                     /gene="yghS"
                     /locus_tag="ACHK1J_003862"
     CDS             2567..3280
                     /gene="yghS"
                     /locus_tag="ACHK1J_003862"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7913072.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPIIGASFGRYLRGKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3312..4070
                     /locus_tag="ACHK1J_003863"
     CDS             3312..4070
                     /locus_tag="ACHK1J_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913073.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4116..5465
                     /locus_tag="ACHK1J_003864"
     CDS             4116..5465
                     /locus_tag="ACHK1J_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO7913074.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAAHELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5465..6289
                     /locus_tag="ACHK1J_003865"
     CDS             5465..6289
                     /locus_tag="ACHK1J_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913075.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQARFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            6301..6861
                     /locus_tag="ACHK1J_003866"
     CDS             6301..6861
                     /locus_tag="ACHK1J_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7913076.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLDKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6892..7962
                     /gene="lptF"
                     /locus_tag="ACHK1J_003867"
     CDS             6892..7962
                     /gene="lptF"
                     /locus_tag="ACHK1J_003867"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7913077.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTDNRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWIVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7959..9038
                     /gene="lptG"
                     /locus_tag="ACHK1J_003868"
     CDS             7959..9038
                     /gene="lptG"
                     /locus_tag="ACHK1J_003868"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7913078.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSLGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQAMQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKMWILHGVNHKKWLNGKETL
                     DTSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQY"
     gene            complement(9073..10245)
                     /locus_tag="ACHK1J_003869"
     CDS             complement(9073..10245)
                     /locus_tag="ACHK1J_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7913079.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10245..10493)
                     /locus_tag="ACHK1J_003870"
     CDS             complement(10245..10493)
                     /locus_tag="ACHK1J_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7913080.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10525..11439)
                     /locus_tag="ACHK1J_003871"
     CDS             complement(10525..11439)
                     /locus_tag="ACHK1J_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7913081.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGIAGGYDW
                     QRVQQLAAEARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11436..13124)
                     /locus_tag="ACHK1J_003872"
     CDS             complement(11436..13124)
                     /locus_tag="ACHK1J_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO7913082.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHTWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         3162 a         3265 c         3431 g         3332 t
ORIGIN      
        1 tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata acactctttt
       61 tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga taatgcgtcc
      121 gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata
      181 tatacacagg gcttttgtta ttacttgctc tggcatttgt gttgttctac gaagcaatca
      241 atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt gccatgcaac
      301 cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta ttgggcggac
      361 ttagcgttgc ctatgccatt gtccatatgt taccaaccga tttgttgctg aatatggggt
      421 caacccacgg tctggcgatg gtcttttcca tgctgctagc ggcgattatc tggaacctgg
      481 gaacgtggtt ctttggttta ccggcctcca gttcgcatac cttgattggc gcgattatcg
      541 gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc
      601 gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg
      661 cgggaggcct gatattcctg ctgcgacgct actggagcgg aacgaaaaag cgtgaccgta
      721 ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaagccg ccattctgga
      781 cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcacacggc gctaacgacg
      841 ggcagaaagg gatcggcctg gtgatgctgg tactggtggg gattgcccct gctggcttcg
      901 tcgtcaatat gaacgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc gtcaccaact
      961 tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattacg atggaacctc
     1021 cattgcctgc ggcatctact gatggcgcgc aagtgacgga gtttcactgt catccggcaa
     1081 atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg
     1141 agccgttaag cgtgagtcag cgcagccagt tgcgccgcat tatgctgtgc atctctgata
     1201 cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa
     1261 aacttcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc atcatggcgg
     1321 tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg
     1381 gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca caaatgacgg
     1441 cggcagtgtc tatcggtctt gccagttata ttgggatgcc cgtctccaca acacacgtcc
     1501 tctcgtctgc agttgcaggg acgatggtgg tggacggcgg tgggttacag cgtaaaacgg
     1561 taaccagcat cctgatggcg tgggtattta ctttaccggc ggcgattttt ctttctggtg
     1621 ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg gtcctggcga
     1681 tcgggcattt ccatttttgg ctagtgataa ccacgcgcgg tcataaaatc cgtaatcgct
     1741 ttttctgcat caaccagcac ctgttccagt ggctgattgg catcgatatc aaccagttgt
     1801 gcaccaccaa aggttaactg tggcgttatg gcaatcttcc tcgccagcga ttcccgttta
     1861 tggtcgggtt tacgcatgca ggcaacttca aggtcaacat tgagtttgat gaccagatca
     1921 ggcttatggc tcgccatcca gtgaaacgct ttacgttcct ggcttgccag ccatgagaca
     1981 aaacgaccac cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc aggaatttgg
     2041 tcctgaggat aacggtcggt tagaacaatt aacccgcgac gacgacaggc aagcatatga
     2101 cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg aactggtcct
     2161 ggcaattttt ttgctgtttt cactttattg cgttcaatag atttattaag cgattttccc
     2221 attaagggca gttttaccac tgcacgaccg acatttccgg cttgttttcc gagatgaact
     2281 ctttctgcgg cgccatattt ttcgacaacg gtaatgagat gttcacacac cgttgacttg
     2341 cctgaaccat cgctaccaat aacggctatt aatggaggtg ttattgactg catgaatata
     2401 tccttaaatt taaatatcca tccaaaatat ttatctggtt aatatgtttt tatgaaagcg
     2461 taattcaggt caatgtcaca attaaccatt gtcacaataa ggttgaacgg atattctgag
     2521 cacggaccac gtaatattca atacattatt tgctgccggg tttttcgtga atcaacgtaa
     2581 tatgtcaata attaattcca cgccagtgcg tgttattgcc attgtaggat gtgatggttc
     2641 aggtaaatcg accctcacgg caagcctggt aaatgaactg gcagcaagaa tgccaacaga
     2701 acacatttat ctcgggcaat cgtccgggcg aattggcgaa tggatttcac agctgcccat
     2761 tattggcgca tcttttgggc gttatctgcg gggtaaggca gctcatgtgc atgaaaaacc
     2821 ctcaacaccg cctggcaata ttactgctct ggttatctat ctgctttcct gctggcgggc
     2881 gtacaagttt cgcaaaatgt tgtgtaaaag ccagcaaggc tttctgctca tcaccgaccg
     2941 ctacccgcag gttgaagtgc cgggttttcg ctttgatggc ccgcaattgg caaaaaccac
     3001 gggcggtaac ggttggataa aaatgttaag gcagcgcgag ctgaagctgt accaatggat
     3061 ggcgtcttat ttgcccgtat tgttgattcg tcttggcatt gatgaacaaa ccgcgtttgc
     3121 gcgtaaacct gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact
     3181 gacattcaat ggcgcaaaga tccttgaact ggatggtcgg caaccagccg atgaaattct
     3241 gcaagcgtca ctacgcgcaa ttcacgccgc gctttcctga tcaccaaaat ggatagggga
     3301 tgatttaacg aatggacgca ctacaaacac aaactgttaa tagtacaacc gcgccgcagc
     3361 ccaactacat tccggggctg attgcggtgg tcgggtgtga tggcaccggt aaatccacac
     3421 tgaccaccga cctggtgaaa tcgctgcaac aacactggca aaccgagcgg cgctatctgg
     3481 ggctgctctc cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt ggcgtctggc
     3541 tggaacggcg actggcggcc aaatcctcga aaacccaaag catgaaaacc aaatctccgg
     3601 cgctatgggc ggcggtgatt atgtactgct tctcgctgcg aagaatggcg aatctacgca
     3661 aggttcagcg actggcgcaa agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg
     3721 aaatttcggg cttttattat gacggaccgg ggattggcgt cgaacgtgcg accgggaaaa
     3781 tcagcatgtt tctggcgcag cgcgaacggc gtttatacca acaaatggcg caatatcgcc
     3841 cggaattaat tattcgcctg ggcattgata ttgagactgc catctcccgc aagcctgacc
     3901 atgactatgc cgagctgcag gacaaaatcg gcgtcatgtc gacgattggc tataacggca
     3961 caaaaattct tgagattgat tccagagcgc cctacagcga agtgctggaa caggcacaga
     4021 aggcggtttc tctggttgcc atcgtttctg accgccgaag tttaacttaa ggctggcggg
     4081 atacttttta ctatcaacga taaggtcagg ctgaattggc gggttttaac atcaaacatt
     4141 ggtttgcaga tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta ggctccagta
     4201 atgtcgtgag cgccttgctg ggtctgttgg cgctctcgtg tgccggtaaa gggatgacgc
     4261 ccgccatgtt tggcgtactg gtgattgtac aatcgtacgc caagtcgatc agcgatttta
     4321 ttaagtttca gacatggcag ctggtggttc agtacggaac accagcatta accaacaata
     4381 atccgcagca atttcgcaat gtcgtctcat tttccttctc gctggatatc gtcagcggcg
     4441 cggtggcgat tgtcggcggc attgccttac tgccattcct ttcccattca ttaggtctgg
     4501 atgaccaaag cttttggctg gcggcgctgt attgcacgct cattccttca atggcttcct
     4561 ccacgccgac cggcattctg cgtgcggtag atcgctttga tttgattgct gtacagcagg
     4621 cgacgaaacc ttttctgcgc gcagcgggga gcgtcgcagc ctggtatttt gactttggtt
     4681 ttgcgggttt tgttattgcc tggtacgtgt cgaatctggt tggcggcacc atgtactggt
     4741 ggtttgccgc gcacgaatta cgccgcagaa atatccataa cgccttcaaa ttgaatctgt
     4801 ttgagtctgc ccgacacatt aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc
     4861 actccatctg gtcggcgcgt aactcgtgca gcacagtgtt ggtggggatc gtgttaggac
     4921 ccgctgccgc cgggttattt aaaatcgcca tgacattctt cgacgccgcc ggaacgccag
     4981 cgggtttact gggtaaaagt ttttacccag aggtgatgcg tttagatccg cgcaccacca
     5041 gaccgtggtt gttaggcgtg aagtccggtt tactggcggg cggaatcggt attctggtgg
     5101 cgctcgcggt gttgatcgtc ggcaagccgc taatttcgct ggtgtttggc gttaagtatc
     5161 tcgaagcgta tgacctgatt caggtgatgc tgggcgcaat tgtgatctcg atgctgggct
     5221 tccctcagga gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa
     5281 ccatcgcctc aatcggctac atcgtattgc tgttcatgtt ctgtcatctg tttggcgtgc
     5341 tgggagcagc gtttgcttat ttcggcggtc aatgtctgga tgtggtgctt tcgctaattc
     5401 cgaccttaaa ggcatttttt cagcgccatt ccttgcttta taacgcagcc ggagagaaat
     5461 cctgatgatg agaaaatatt tcccgcttga ggcgagcgag cgtctatttg ttgcgattga
     5521 agaagatgat gtcgttgatg cacaggtcag tttgccgccg actatcgcac taagttgtac
     5581 caccgaaatc atccatgata attacgcgtt atgcctgcaa ttctggctca atggcgttga
     5641 tcgtcaggaa ttacttcgtt taattcgcaa acaagccaaa ggcgatgagc tgacgacaga
     5701 cgagcgtaaa cagtttaaat acatgcgagc gcgctacaag catctacgtt ttgcacaacg
     5761 gctgtattta aaaaagcatc aggccagatt tttattcggc aaaacgaccg tttttctggg
     5821 acgttttcag gacggatttc gtaacggtaa gaaaaatatt gtgtcgtatt acggcaacct
     5881 gttacgaatt tatttaagct ctccggtctg gtcgttggtg aactattcat tacgccatag
     5941 ccagttagaa agcgtaagcg gttttattgc ctatcggcaa aagcaaatgc ataccctgaa
     6001 agagattatt gccaaaccgc gactaaccgg cagggaattt catgatgtgc gaaaaatcat
     6061 cagccagcag gtttcttatt acgacacctt aagatcgctt gatcctgaaa ataaagaggc
     6121 gttacagatt tcccgtttcc tcgccgccat taatggcctg atgggggata aacacgacga
     6181 catggtggcg gatgatatgg aaaaccgcca gtcgtatgat gcaccggtgg cgctggatag
     6241 cgatattcgc cagcgcttag agctgctgat ttcgcgtttt ccgctataac cggagcaatg
     6301 atgtcatctc gtttgattat tgcgctgatt atcatgctct tagccccggg tgtgcaggca
     6361 cacaatttcg tcactggcaa aacggttacc cctgtttata ttcaggaagg cggcgagctg
     6421 cttctgaata gcgacgacga aattcactac caaaagtgga acagcacaca attggcggga
     6481 aaagtccgca ttatccagta tatcgccggg cgtaagtcag cgaagaagaa gaactcactg
     6541 ttaattaaag cggttgaagc ggcaaacttt ccacaggatc gttttcagcc caccaccatt
     6601 gtgaacactg atgatgccat ctttggtact ggctattttg ttgttggcaa aatcgaaaaa
     6661 aataaacgcc gctatccgtg ggcgcaattc gttattgacg gcaatggaca gggacgcgtt
     6721 gcctggcgct tgccggagca gagttcaacc attctggtgc tggataaagc cgggcaaatt
     6781 cagtgggcaa aggacggcag cctgacgccg gaagaggtag atcatgtcat tgcgctggcg
     6841 cagaaactga tcaatgaata aaaaattcaa aggattgagg gcgttagtga gatgaagctg
     6901 gttgaacact acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt cggttttctg
     6961 atctttattt tcgccagcta ctccgcacag cgttatctga ccgaagcggc aaatgggaca
     7021 ttagcacttg atgttgtgct ggatatcgtt ttctacaaag tgctgattgc actggagatg
     7081 ttgttacctg ttggactgta tgtgtcagtt ggcgtgacgc tagggcagat gtataccgat
     7141 tcggaaatta ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta caaagccgtt
     7201 ctttatctgg cgataccgct aagtattttt gtcacccttc tgtcgatgta tggtcgtccg
     7261 tgggcttatg cgcagattta tcaactggag caacagtcgc agtcggagtt ggatgttcgc
     7321 cagttgcggg caaagaaatt taacactaac gataacggac gaatgatcct ttcgcagacg
     7381 gttgatcagg ataataatcg cctgactgac gcgctgattt atacttctac tgacaatcga
     7441 acccgcattt tccgcgcccg ttcggttgat gtggttgacc catctcccga gaaaccgacc
     7501 gttatgttgc acaacggcac tgcctatctt ctcgatcatc aggggcgtga cgacaacgaa
     7561 cagatctacc gtaatctgca attacatctg aatccgctgg atcaaagccc taacgtcaaa
     7621 cgcaaagcaa aatcggtcac ggagctggcg cgctccgcct ttcctgccga tcatgccgaa
     7681 ctgcaatggc gacaaagccg tggcctgacc gcattgttga tggcgctgct ggccatttca
     7741 ttaagtcggg taaaaccgcg gcaagggcga ttttcaacgt tattgccact gacgttgctg
     7801 tttgttgcca ttttttatgg cggcgacgtc tgccgtacgc tggtggctaa cggtgcgatt
     7861 cccctcattc ctggtttgtg gatagtaccc ggactcatgc taatgggcct gctgatgctg
     7921 gtcgcacgcg acttctcttt gctgcagaaa ttttcccgat gaatgttttc agtcgctatt
     7981 taatccgtca tctctttctc ggttttgccg ccgccgcagg gttattgctg ccgcttttta
     8041 ctaccttcaa cctgattaac gaactggatg atgtcagcct gggcggttat cgctggactc
     8101 aggcggtgct ggtggtgctg atgaccttac cgcgcacact ggtggagctt tcgccgttta
     8161 tcgccttatt gggagggatt gtcggcctgg ggcagctatc gaaaaacagt gagcttaccg
     8221 ccattcgcag cacggggttt tctatcttcc gtattgcact ggtggcgctg gttgccggga
     8281 tattgtggac tgtttcgtta ggcgcgatag atgagtgggt agcgtcgcca ttgcagcaac
     8341 aggcgatgca aatcaaatcg accgccactg cgttggggga ggacgatgat attaccggca
     8401 atatgctgtg ggccaggcgc ggtaatgaat ttgtgacggt gaaatcgctg aacgagcagg
     8461 gccagccggt gggcgtggag atatttcatt atcgcgacga tctctcgctc gaatcctaca
     8521 tttatgcacg cagtgccacc attaaagacg acaaaatgtg gatcctgcat ggtgtgaatc
     8581 ataaaaaatg gcttaacggt aaagaaacgc tggacacatc agataatctt gcctggcaat
     8641 cggccttcac cagtatggat cttgaagagc tatcgatgcc ggggaatact ttttctgtcc
     8701 gtcagcttaa tcattacatc cattatttgc aggaaaccgg gcaacccagc agcgaatacc
     8761 gccttgcact gtgggaaaaa ctggggcaac ccatcctgac cctggcgatg attttgctgg
     8821 ctgtgccgtt tacctttagc gccccgcgct cgccagggat gggtagccgt ctcgctgtag
     8881 gcgtcatcgt tggcttactc acctggatca gctatcaaat catggtcaat ctgggattgc
     8941 tatttgcgtt aagcgcacct gttaccgcgc tcggtttacc gatagcgttt gtgctggtgg
     9001 cgttgagcct ggtgtattgg tatgacagac aatattaaac ccgccgtggc gcaacaccac
     9061 ggcgggctca cattactgct tcagagtagc aaatgcctga atgatggcat cgatttgctc
     9121 acgactgtgg gcagcgttta cgctacagcg cagcagcgtg atgcctgcag gcgcggcagg
     9181 tggcaggata agattcacat aaacgcccag cgaaataagc tcgcgccaga tgcgtaaacc
     9241 gtcctctttc gaaccgatta ttaccggcac taccgggcta atatgtgaac ttaactcata
     9301 acccagtttt tgcagaccgt catacagatg gttggcatta tccatcagct tttgccgcag
     9361 ctctggttgt gaggcaatgg ttcttaacga cgagcgcacg gtcgcaatgc aggacggaga
     9421 aggagaggcg gtaaaaatat acgggcggct gccgtagcgc agcacttcca tcgcttccga
     9481 gcctacagca aaaccgccaa tcgaggccag acttttactg aaggtgccaa cgataatatc
     9541 cacatcatct tcaacgccga cagcttcagc cagtccgcgc cccgttgcgc ctaacacgcc
     9601 aaacgaatgc gcttcatcga caatcaaata accaccgaga cgacgtttga tatcaacaat
     9661 ttccgccagc ggcgcaacgt caccgagcat actgtagatg ccctcaacga tgataatcgc
     9721 ctctttcgcc cgttcaccga ggcgaaccat gcgccgttcc agatctttag catcgttatg
     9781 gcggaaacga ataatttccg cgccaccgag agaacaggcg tcataaatac tggcgtggct
     9841 gtcggcatcc agtaatacca cggcgttatg gtcggcaagt gcgctgatga cgcctaaatt
     9901 agcggtataa ccggttgaaa aaacgatagc ggtagggcga ttgaaaaact cggcaatctc
     9961 tttctctaac gccaggtgcg gcgcataact gccgtttgcc atacgagaac cggtggtgcc
    10021 ggtgccttgg gccgctaacg ctgcctgacc ttcggcgatg gcatcatgat taaaggttaa
    10081 accgaggtaa ttattggtcc cggccagaat cactttttta ttgccgatcc ggccttcggt
    10141 ggcggaatac acttcatcaa tacaggtgcc aaacggtgtt aacccgttgt cactaaattg
    10201 cagtcgctcg ccagcaaggc gcgcatattt atcgtatagc cccattattt attctccagc
    10261 catggaagta gagtttccat taattgcgca ggggttttga catccagcag aatattaata
    10321 ggaatggaaa tatcgaatct gtcttccagc atcattaaga gatccatcac tttgatcgac
    10381 tccaggccaa gatcgttgac gagatcgctg tcgggcttaa tttccacacc gttttcgacc
    10441 aaatcctgta aacaggaaag gatgtaatcc attactattt cacgatttac catagaaacg
    10501 ttaacacacc tttaaacaag acaatcaaaa tagcccgttg cgtaatgcgt gttccaggct
    10561 aatcccggga aaccaattaa tatcttcaga aatacggtta ttacttgccg accagtcggc
    10621 gtgggttaat tcacgaattt tcgagcgggt cagcataggt tctttacctg ccaggcgact
    10681 caacgcggta ctgatatccg caaggcaggt gagtaccggc agaggaatgc caaccatccg
    10741 cacagaacca caacgggctt cggcggcaag ttgctgtacg cgttgccagt catagccgcc
    10801 agcgatgcca tcgcataatt cataggtttg cgtctgtaca gtttcggcgc ttaaccactg
    10861 acccactgct tgcgcaaaat cggtgacatg caggaaagag agctgtgtat ctggtgcacc
    10921 aagtcgtggc agcaggccgc gcagcatcca gtcaaacagc ggttttaact ctttatcgcc
    10981 gggaccatac acggctgtcg ggcgaaaaac gcccagcgta atttcgtcag ccattgccgt
    11041 cagccgttgt tcggcgacgt gtttggaatt tgcgtaccag gagagctcgg gatggcgcgc
    11101 cgccagcgaa gagataaaca gaaaacgttg gcaaaagccg ctctcttttg ctgcttgcat
    11161 caggcgcagg ctgccgtcaa cgttacagtg ggtaaaaatc tcttctttgt gtccgcgcac
    11221 ttgcccggcg caatggacca ccgcgctggc tccggcaacc agttcgctaa gcgaatgtgt
    11281 atcttccagc gaaccgcgca cccaggtgag attgtcattg acgtgagcgc gggcagtacg
    11341 cgtcaatgcg cgaacgtgaa agccgcgggc gagcaggtta tcgataatat atttaccgat
    11401 aaacccggta gcgcccgtca ccgcgacagt ttgattcatg ccagggattc ctgcacatga
    11461 agactggcag cataagcctt ctgataacgt tttttcgctt ccgcacgggc aggcttgccg
    11521 gaggacgttc ggggaatact gtggggcggc aacagatcga tagccgcggt cacgccaaat
    11581 tcgctttgga tccgtgctgc cagcgcgtgg ataagctgcc cgcgacgttc ttcgtcgctg
    11641 atccgacact ggatctgcaa aatgattttt tcctgggcgg taacaaaagc aatcgcatcg
    11701 ccagaatgaa tttccggttc ctgttccgct atatattcaa tatcctgcgg ccagatatta
    11761 cggccacgaa taataatcag atctttaatg cgtccggtga catacagata accgtccagc
    11821 agataaccga ggtcgccggt gtctaaccag cccgtcgcgg caatctcgtc ttgcgaagcc
    11881 tggtcgccaa agtaaccgct catcagactg ggaccggaga tgcaaatatg gcctaccaca
    11941 cgttccgcga ccggcatacc tgcttcattg cggatttcaa tgccatgttc tggcaacgct
    12001 ttgccgcagt tgacgaaagt cgatacggcg cgtgtctctg cacccggcgc gacggcttta
    12061 ccctgatatt cgaggatgtc gcgatccact tcgttaacca caaccccgga ggcttcatca
    12121 gagaagctga cagccagcgc attttctgcc agtccgtagc acggcatgaa agttttattg
    12181 tcaaagttaa cctgacggaa acattcagcg aattgatgga gttgttctgc ggagatgggt
    12241 tctgcaccaa taccagcgac gcgccagcag gaaagatcca gttcagcgag atctttttca
    12301 ttcacgcggc gctggcacaa ttcatagcca aacggcggcg caacggaaac ggtgccgcga
    12361 tttttactga tcaatttaag ccattgcaga ggacgcatgg caaaatcctg agtgcgcaaa
    12421 taatctactg aaagttgcgt ggcgacgggg gtcaggagaa agcctaccag tcccatatca
    12481 tggtagaaag gcagccagga gacgcagcgg tcgccagggc gtaatttaat gccgtcgtgg
    12541 cttatagcac gtagattagc catcacttcg cgatgggtga taatgacgcc acggggaaaa
    12601 cgggtgctgc cggaggtgta ctggaggtag gcgatatcgt tcggaactgg acgctggagc
    12661 gcaacatcgg cttccggtaa tgccttaaac caggtgtggc ttaatacatg caattcgggg
    12721 ttgttatgcg tggcggcatt gaccagcggc aaccactcat caccagtgat aatggcagcg
    12781 ggctggcaac ttgccagtaa accctgcagt ttggcgctcc aggaatcccg ctgaccgacg
    12841 cccattggaa tcgccaacgg gacggcgact aagccggcat actggcaggc aaaaaaagcc
    12901 tctacgaacc cgctactggt ttcggcaatc agcgccacgc gatcgccttt tttcaggttc
    12961 agcgataaca accgcttcgc accagcttcg gcacgtgctt ttaacgtctg atattccagt
    13021 tgatcttcaa gttggcaacg tctgtcataa aaattcattc cagcgctact cagagcggcg
    13081 tagtccaaag catcaaccag cgttggaaaa tcggcatagc gcataggtag ggaatgcgta
    13141 aagattttat tagacatata caccacgaaa aaattggata tcaaaaataa
//
LOCUS       DAZGBC010000140        13124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGBC010000140 DAZGBC010000000
VERSION     DAZGBC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.141"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..462)
                     /gene="yhaH"
                     /locus_tag="ACHK1J_003873"
     CDS             complement(97..462)
                     /gene="yhaH"
                     /locus_tag="ACHK1J_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7913083.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(755..1741)
                     /gene="yqjG"
                     /locus_tag="ACHK1J_003874"
     CDS             complement(755..1741)
                     /gene="yqjG"
                     /locus_tag="ACHK1J_003874"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO7913084.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1811..2203)
                     /gene="yqjF"
                     /locus_tag="ACHK1J_003875"
     CDS             complement(1811..2203)
                     /gene="yqjF"
                     /locus_tag="ACHK1J_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7913085.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2389..2688)
                     /gene="yqjK"
                     /locus_tag="ACHK1J_003876"
     CDS             complement(2389..2688)
                     /gene="yqjK"
                     /locus_tag="ACHK1J_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO7913086.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2678..3082)
                     /gene="yqjE"
                     /locus_tag="ACHK1J_003877"
     CDS             complement(2678..3082)
                     /gene="yqjE"
                     /locus_tag="ACHK1J_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7913087.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3085..3390)
                     /gene="yqjD"
                     /locus_tag="ACHK1J_003878"
     CDS             complement(3085..3390)
                     /gene="yqjD"
                     /locus_tag="ACHK1J_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO7913088.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3428..3796)
                     /gene="yqjC"
                     /locus_tag="ACHK1J_003879"
     CDS             complement(3428..3796)
                     /gene="yqjC"
                     /locus_tag="ACHK1J_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO7913089.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3943..4326)
                     /gene="mzrA"
                     /locus_tag="ACHK1J_003880"
     CDS             complement(3943..4326)
                     /gene="mzrA"
                     /locus_tag="ACHK1J_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO7913090.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4330..4992)
                     /gene="yqjA"
                     /locus_tag="ACHK1J_003881"
     CDS             complement(4330..4992)
                     /gene="yqjA"
                     /locus_tag="ACHK1J_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO7913091.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5014..5097)
                     /gene="yqiM"
                     /locus_tag="ACHK1J_003882"
     CDS             complement(5014..5097)
                     /gene="yqiM"
                     /locus_tag="ACHK1J_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO7913092.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5337..6113)
                     /gene="exuR"
                     /locus_tag="ACHK1J_003883"
     CDS             complement(5337..6113)
                     /gene="exuR"
                     /locus_tag="ACHK1J_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO7913093.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQTADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6243..7541)
                     /gene="exuT"
                     /locus_tag="ACHK1J_003884"
     CDS             complement(6243..7541)
                     /gene="exuT"
                     /locus_tag="ACHK1J_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO7913094.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            8024..9436
                     /gene="uxaC"
                     /locus_tag="ACHK1J_003885"
     CDS             8024..9436
                     /gene="uxaC"
                     /locus_tag="ACHK1J_003885"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO7913095.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9451..10938
                     /gene="uxaA"
                     /locus_tag="ACHK1J_003886"
     CDS             9451..10938
                     /gene="uxaA"
                     /locus_tag="ACHK1J_003886"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO7913096.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQKRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11021..11572
                     /gene="ygjV"
                     /locus_tag="ACHK1J_003887"
     CDS             11021..11572
                     /gene="ygjV"
                     /locus_tag="ACHK1J_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO7913097.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(11576..12820)
                     /gene="sstT"
                     /locus_tag="ACHK1J_003888"
     CDS             complement(11576..12820)
                     /gene="sstT"
                     /locus_tag="ACHK1J_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO7913098.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3338 a         3360 c         3357 g         3069 t
ORIGIN      
        1 acattgaaat attttaaata aacattagta aattattcat tcatcaacat gatgataaaa
       61 gccggatgag tcagacatcc ggcgagcaga tttgttttaa ggttccagtt ttggatcagg
      121 tccaaagcgg ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca
      181 gccgataaac gggattaaga atagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag
      241 gcggcggaac tgaactgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag
      301 gatgccttcg ccaccagcgc gttgccagcc taacatttta tccagcagcc ccagcacgaa
      361 cgtaaagatg atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg
      421 gaaaccgaca taatttttta gtactttcag ataccagtcc atttttgcct caattttgct
      481 caatcacttg tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata
      541 ttcgttcaag gaattaataa agtgctgtga aggcgcgcta agactgatga caagcgcaaa
      601 attgccagat gcgctacgct tatctagcct gcgcggtcat tgcaacatat tgaatataaa
      661 agttttgtag gtttgataag gcgttcatgc cgcatccgac atgaacaaca tgcacttagc
      721 taattatcag aggcacctcg taaggtgcct ttttttaacc gaagcgaaca tctcgtccat
      781 gcggttcatc gagatcctgc cacggaccaa ttgaaataat ccccgtaggg ttgatggtct
      841 tatgactgcg gaagtaatga ttacggatat gatcgaaatt gactgtttcg gcaattcccg
      901 gcatctggta gatatcacgc aggaagccat acagattaag gtaatcgctg atgcggtgct
      961 tatcacactt gaagtgggtc acatacactg gatcaaaacg gaccagcgtg gtccacaggc
     1021 gaatatcggc ttcggttagc tggttgccgg tcaggtaacg atgctgacct aaaatctgtt
     1081 ccagtcgcgc cagcgattca aacactttct ccaccgcttc atcgtaggct tgctggctgg
     1141 tggcaaaacc agctttatac acgccgttgt taacggtgtc ataaatccag ccgttaagtt
     1201 cgtcaatttt cgtttgcagg gctggtgggt agtaatcacc cgctttcgcg cccagcgcat
     1261 caaacgcggt attaaacatg cggatgattt ctgctgattc gttgctgacg atggtgtggt
     1321 tctttttgtc ccacagcacg ggaacagtaa ctcgtccgct gtagtgtgga tcggcgtgga
     1381 gataaagctg atacaaaaat tcatgttgat agagcgtgtc tccggttgct cccggaaaac
     1441 tgtcgtcaaa ggtccagccg ttttccagca tcagcgggtt cactacggaa acggaaataa
     1501 acggttccag tcctttgagt ttgcgcatga tcagcgtgcg gtgcgcccac gggcaggcga
     1561 gtgaaacata gagatgataa cgatctttct ctgcgataaa accgcctgtg ccagtggggc
     1621 ctggagcgcc atcggcagtg agccagttac gaaatgcgga agctgaacgt tgaaatttac
     1681 cgccggtaga tttggtatcg taccaggtgt catgccagac gccgtcaatc agttgaccca
     1741 tttttctctc ctccaatagc aaaaacgagg agcttcctcc tcgtttttta tcgttaagta
     1801 tagcgcgtgc ttaccacttt ttattcagca ggcggtcgat gctatacgcg cccggaccgg
     1861 taattgccag cagcaggaat ccgccagaaa ttgtcaggtt tttcatgaac atcagcgagt
     1921 tgacgccttc agcaaagttg ctgtgaaata aaaatgccgt cagcagcgta aagcccgcag
     1981 taaacagggc tgtggtgcga gtcaggaaac cgaacaggat tgccagacca ccaccaaact
     2041 caagcagaat caccagcggc agcataaaac ccgggacgcc catcgcttcc atatattgtt
     2101 gggtacccgc gtaaccagta atttttcccc agcctgcggt aataaacaga atcggcatta
     2161 aaatgcgcgc taccagtaca ccaacatctt ctaatttttt catcatactc tccactgcgc
     2221 cactcaggct gctgattgtt tttttgtgca agggcgcggt attagcgtcg ttgctgtcga
     2281 tggagagaat cataaacgtg gtgaatgatg attgttagca aggaaaactg tcaaaaatct
     2341 tcaaaaaatt tgatgggtaa ggccggaatg gctccggcca gagagaagtt aaccgcgaag
     2401 ctgttgctgc ttgagggtcg ttttaaccag acgccaggcg ctccatacgc caaaaccgcg
     2461 tctggcccag cggaccagca tattaggatg gcgaatcgtc cagatcgcca tcacgctact
     2521 gccaaccagc gcccaggagc gcagacttag cagcatattc cagcgacgat cgtaagcgcc
     2581 tgttgcctcc agccattcac gacgactggc agaaagatcc agccgttgtt gctggatctg
     2641 gctaagcagt tgcgccttac gtcgttcacg ttcgacttta ctgctcacgg gactcctcct
     2701 cgagcagctg ccgatcgttt gccagctcat ggcgtgtatg gcgcagcaag gtggatttac
     2761 gcgatttacg taacgtccag ataccgccaa tcagtgccag tagcagcaac accacggtgg
     2821 tggcaatcat cgcattcagg cgatattgcg gatcaaccgc ccaaataatt agcaccatca
     2881 ggctcataag accaaatgca gcgaaaagca tcgtcaggcc cagcatcagt aaaagttgaa
     2941 agagattagc tttttcctct tccagctcca ccaccgccag ccgcagacgt gtttccacca
     3001 tttcaaccat gatggaaaca attcgctgcc cgatgcccag aacgctttta ccgggccctt
     3061 gcgcgtgatg agtgtccgcc ataattaacg acgcgacagc agaacgccga gcactacacc
     3121 gattgcagcg ccaatgccca cgcccgtcca tggattttca cgcacatact catcggcacg
     3181 cgccgccgcg acacgggttt gtttggcgat ggcatcaccg gtttcaccca ggcgataacg
     3241 gctctgtttc agtgcctgct ccgctttgct acgaatctta ctcaactctt ctttcgactt
     3301 ctcgccagat gagctaagca cctcttccag cgtatcggaa agggatttca actcagcacg
     3361 cagatgttcc gtagtgtgtt ctttcgacat agttttctcc aggtgagtaa gtagtgacta
     3421 ttgtgagtta gtagtcgcgc gcttccagct ttttcagctc ttcctgcgct tctgccagtt
     3481 tccgttcgcg tttggcaatc ttatcggcat cgcctttttg cttcgcctcg gctaaatctt
     3541 gctggcgttc cgccacctca tctttctgct ttgcgatttt cttctgatga tcggcacgca
     3601 gctggctatc tgaacagttg gcccggactt cactcagggc tttattcaga ccgtcaatac
     3661 gattctggtt ttggtgtttt tcggcatagc tgatctcctt aaggatattt tgctcctttt
     3721 cctgacacag ggtagtggca taactaccgg cactaagagc aaaaagagaa acagctaaag
     3781 cgatgcggta tttcattctt gaaccttcca ttgtgattcg gacctattac aaaggaacga
     3841 ttgtccagac aacgaagcgg tagtgttaac caccgcctca ttaatcatag acagttacgg
     3901 tgaaatcacc aaagtgagtg atctgtttcg gataacagga agttatccga agcgatgtga
     3961 gttatcccgt agccgggtca gccactgcat agcctgactg ttattgtcct gctgcgcaat
     4021 gatgtagcct tggggcaatg ttctgtcgag gaccgctttt gcagcggcgc tctggtcgct
     4081 ggagtcaaag gtaattaaca gagtgtcgtt tttgggggtg atacttttga aaggaatgcc
     4141 atgagcgtca aggtgatgcc agattgaaaa accgtctggc atcgttgtgc cttgatgaac
     4201 ggcacgaatc gccagcgtag actcttgctg gcgaaccgcg gaccaggcca gcaacagcgt
     4261 gccgaccaga agtaaaacag cgccggacca ggctagctgg cgaagcgaca tgcgaggtat
     4321 ttgcatccct tacccccgat ttccatattt ctttttccat aacacgacca gagaacctgc
     4381 caggccaaac accagcagca ccaccgggag cagcatcagg catgacatca gctggtcctc
     4441 gtactttaaa aataccggcg ttttgccgag catgtaaccc agagttgtca ggatcaatac
     4501 ccacagcaga ccgctcatcc agttgaaaaa ctgaaagcgc gcgttattca gccctgataa
     4561 cccggcaatc gtcggcagca gtgttctgac aaacgcaata aagcgaccaa ttaacagcgc
     4621 cgataaaccg tgtttatgaa aaagatggtg tgcgcgttga tgataatgcg cgggtaaatg
     4681 agatagccag ttttgtacgg tgcgggtatt gcccagccat cgcccctgaa tatagctgac
     4741 ccagcagccg aggctggcgg caacggtcag cagcagaatc gtttgcggat agcccatcgc
     4801 gcctttcgca atcaacacgc cgaccaatac cagtaaactg tcgcccggta aaaaggccgc
     4861 cggaagcaag ccgttttcaa ggaacaaaat tacaaacaag acaaaataca acatgccaat
     4921 catcgatgga ttggccaggg tttcaaaatc ctgcgcccac agggcctgca gcaattgggt
     4981 caaaagttcc attcattatt cctggtgaat cggttaacgc cacgctggtt ttaaaagatg
     5041 cactaccgca atgttattgc tgttttggta tgttctgcgg gtttgataca tcaacacagc
     5101 ctgaactatt ccctgttaaa tcgtgagata agaaagcatt caaaaacata aattcaaatt
     5161 gaatctaatt gtaacaaagg tcaacgttgt tcgttatcaa aattacatgt ccgtggagtc
     5221 tgattttgct gaatgcaggg gagggtggcg agaggattta cacaggctga cactttatac
     5281 ttttcgctta ccgacgagac aaacaggcgc attgcagcgc ctgtcggaca ggaatttcat
     5341 ttactgccgc tggtggctgt atcgagatgc accaccgggt tttcggcgaa tagatagcgg
     5401 tcggcattga actcgaagtc gtcgctggtt tcgttgaata acatgatctt ggtgttttcc
     5461 aggtgctgcc acattgccag cttagcagca tgaggatctt tgcgaatcag cgccttgagg
     5521 atttgatcgt ggtcatcaca ccagttatcg acggtacggg aatcaatgtg ttcgtgcagt
     5581 tttttccagt acgggttatg gctacgctgg gtccacattt tttcaacgat agccgccagg
     5641 gcggagttct gcgtcgccag ggctacctga atatggaact gcaaatccca ctcggaatca
     5701 cggaagcatt gttcaccgcg tgcctgctcc tggatggcca tcaatttcat gatgtcctgt
     5761 ttcgttacct gagttgccgc gaactcggca atattacttt cgatgagctg gcgagcctgg
     5821 agtaactcaa acggaccgta attggcgaat tccatattat tgtccgccgt ctgctgatgg
     5881 cgcggctggt tggaaaccac gtgaatacct gaacctttac gcacttcgac atagccttca
     5941 acttccagca tgatgatggc ttcacgaact accgtacggc tgacgttctt ttcatcggca
     6001 ataaagcgct cggcaggcag tttatcaccc accagataga cgccctgttc gatgcgctct
     6061 ttcaggtcag cggcaagttg ttgatacaaa cggcgtggtt cagtgatttc catatgcgct
     6121 ccaggcatga tacggcagac tatatttgtt ataccacttt tgcgagttta gctctactcg
     6181 cagtgatgaa aaagccgcct gagaaggcgg ctttgaaggg aaactaaaac ttacacgaag
     6241 cgttaatgtt gcggcgcggg atcgttatgt gtttcctgcg ccacctcaat cgccggtttg
     6301 ttctgcaaca cggtccagat aaccagcgca cccagcaggt caaatactgc cagaactgcg
     6361 aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta
     6421 cttgccagcc atgcggacat cccggttaaa ccgtttgccg ttgccacttc gttacgacca
     6481 aacacgtcag aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg
     6541 atacacagca gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca
     6601 atcatcagca cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca
     6661 aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac
     6721 agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac
     6781 atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgccag gaaacgcggc
     6841 agcgcgatac cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc
     6901 gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc
     6961 agatgcttct ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag
     7021 ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcatcacgat tgcccatacc
     7081 accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta gcctactgcg
     7141 atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc
     7201 gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca gctacctgcc
     7261 agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata accgattttc
     7321 gtacccagca catccagtac ataacccgct accggttgca tgaccgtata agcagcagaa
     7381 taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt
     7441 ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc
     7501 agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct catcgttttg
     7561 ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc
     7621 gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc
     7681 ggcttccgta ccggttaacc cgattcatct aacaaatgag tgaatcggca atttgcattc
     7741 cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt aaaaggtgag
     7801 agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac
     7861 tggcgcggcc tgcagcgaaa tttaccactt tgagagtaat ttttttaact acgtttattg
     7921 atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc
     7981 gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac
     8041 tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga
     8101 ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg
     8161 ttttaaaaac ctgtatgaca tctggttgaa gggcgatcac tacaaatggc gcgctatgcg
     8221 taccaacggt gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga
     8281 cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcacct
     8341 cgaactgcgt cgtccgtttg gtatcactgg caaattgctt tctccgtcaa ctgccgatga
     8401 aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat
     8461 gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca
     8521 tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc
     8581 ggacaaagcc tttaacatcg aacaggcgac ctttaacgac tacatggcga agctgggcga
     8641 agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct
     8701 ggatcacttc gccgctcacg gctgtaaagt gtctgaccac gcgctggatg tggtgatgtt
     8761 tgctgaagcg aacgaagcgg aactggacag cattctggcg cgtcgtctgg ctggcgaaac
     8821 cctgagcgag cacgaagtgg cacagttcaa aactgcggtg ctggtgttcc ttggtgccga
     8881 atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct
     8941 gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat
     9001 ggcggaagag ctgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac
     9061 cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt
     9121 ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca
     9181 gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtttgc tgagccgctt
     9241 tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg
     9301 ccgcattctg tgccagatga ttggtcgctg ggtggaagcg ggcgaagcac cggcggacat
     9361 caacctgctg ggtgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc
     9421 cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat
     9481 aacgtcgcgg tcgctttagc ggatttggct gaaggcacag aagtcagtgt cgataaccag
     9541 actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca
     9601 aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg
     9661 gcgggggaac acgttcacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat
     9721 cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcgtga agtgcagatc
     9781 tatcgtcgcg ctaacggcga tgtcggggtg cgtaatgagc tgtggatcct gccaaccgtg
     9841 ggctgtgtca acggcatcgc gcggcagatc caaaagcgtt tcctgaaaga gaccaacaac
     9901 gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc acagctgggc
     9961 gacgatcaca ttaatacccg caccatgctg caaaacatgg tgcgccaccc gaacgcaggc
    10021 gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg
    10081 ctgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag
    10141 atcgaggccg ggatcgagca tttgcatcag ttgtataacg tgatgcgcaa cgataaacgc
    10201 gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt
    10261 tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc
    10321 ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac
    10381 cattgccgcg acgaagcaac gtttgaaaaa ctggtcacta tggtcaacga cttcaaacag
    10441 tactttattg cccatgacca gccgatctat gaaaacccat cgccggggaa caaagcgggc
    10501 ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagtgtc
    10561 gtggttgacg tgctgcgtta cggcgagcgt ctgaaaactc cggggctgaa cttgttaagt
    10621 gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg
    10681 ctgttcagta ctggtcgtgg cacgccgtat ggtggatttg tgccgacggt gaaaatcgcc
    10741 accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg
    10801 atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt
    10861 gccaacggta agcaaacctg taacgagcgt aacgacttcc gcgaactggc gatcttcaaa
    10921 agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt
    10981 gaagcgccgt ttgcttttcc atacctgcta aggatttatc atgaccgcgt attggctggc
    11041 ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga
    11101 acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct
    11161 tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac
    11221 cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg
    11281 cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggcacga ttgtcagtac
    11341 ctgggcgctg ttccgctgta aagggctgac catgcgctgc gtgatgtggt tttcaacgtg
    11401 ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacgatga ttgagggtag
    11461 ttttctgctt atgaatggcc tgaatatcat tcgtttctgg cggatgcaga aaaggggaat
    11521 tgatccgttt aaagtagaga aaaccccttc cgccgtagac gaaagggggt aaacattaat
    11581 tacgcagggc gctatttgcc agacgatcgt cttccgcctg gcaagctgcc gcagtgaaca
    11641 gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg ccgatgataa
    11701 agccgacggc aaccacctgc atggcgatat cgttcgaaat accgaacata ttacaggcca
    11761 gcgggatcag cagcagagaa ccacccgcca cgccggatgc gccacaggca cacagagaag
    11821 ccactacgct caacagcagc gctgtgggca gatcgaccgg aatacccagc gtattaaccg
    11881 cagccagcgt caacacggta atggtgattg ctgcacccgc catattgatg gtggcaccca
    11941 gcggaataga aacggaatag gtatcgcgat ccagattcag cttttcacac agcgccatat
    12001 tcaccggaat gttagctgca gagctgcggg tgaagaaggc atacacgccg ctttcgcgca
    12061 ggcacagcag caccagcggg aacgggttac gacgaatttt ccaccacacc agcaatgggt
    12121 taaccaccag cgccaccagt aacatacagc caaccagcac gaccagcagt tgcgcgtagc
    12181 cccacagtgt ggagaaaccg gtggttgcca gggtagaaga aaccagccca aaaataccga
    12241 tcggtgcgaa gcgaatgacc agtttcacca taaaggtgac ggcattcgac atatcgttga
    12301 ccaggttttt ggtggtttcg ttaccgtgac gcagtgcgaa accgaggcca attgcccaca
    12361 ccaggatccc gatgtagtta cctttcagca gcgcgtcaat ggggttggaa accatgctca
    12421 ttaccagtcc gcgcatcact tcgacaatgc ctgacggcgg cgaaatatca cccgcgctac
    12481 tggacaagtg cagggtagaa gggaaggcaa agctgaagac tactgcggcc agagctgcag
    12541 agaaggtgcc cagcagatag aggaacaaaa taggacggat attggttttc tgcccgtgct
    12601 ggtggttagc aatagatgcc attaccagca tcaacaccag gatgggggca acagctttca
    12661 gtgcgccgac gaacaaagta cctaacagac caacagcttc cgccgcgggt tttgagatcc
    12721 atgccagaag aatccccaga acaaggccga ccaggatttg ttttaccagg ctgccatgag
    12781 ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc tttcattgtg
    12841 ttgtcgcaca tccctggtgc gttctgtcga cgcatctttc aggatgtaac aaagtgtttg
    12901 caaggtcgag tataaggaaa gttacagctt cgggaagcga aaaatgctgg attttaaaga
    12961 gaggtcatgt tttgcaactt aatggttaca tttatttgac ataaacagag ggaaggactg
    13021 gctactaagg ctgattagat aacatatggg caaaactatt tggaatatga gaacggttgc
    13081 gctcctttaa tgtgctatgc catataaagt cactggcaca gggg
//
LOCUS       DAZGBC010000141        13017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGBC010000141 DAZGBC010000000
VERSION     DAZGBC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.142"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..1567)
                     /gene="amn"
                     /locus_tag="ACHK1J_003889"
     CDS             complement(113..1567)
                     /gene="amn"
                     /locus_tag="ACHK1J_003889"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO7913099.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYIRSGELPDE
                     NARKQGLFVYPSLTVTWDGGTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1669..2985)
                     /gene="shiA"
                     /locus_tag="ACHK1J_003890"
     CDS             complement(1669..2985)
                     /gene="shiA"
                     /locus_tag="ACHK1J_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO7913100.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            3300..4352
                     /locus_tag="ACHK1J_003891"
     CDS             3300..4352
                     /locus_tag="ACHK1J_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO7913101.1"
                     /translation="MFLASLLRHIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLLIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4614..12617)
                     /locus_tag="ACHK1J_003892"
     CDS             complement(4614..12617)
                     /locus_tag="ACHK1J_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO7913102.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLVTQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALKAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSSATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQVLTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPPGEITVTDTAPQQLTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGIAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVYTAEATLPNGNNDTKMVNIAPDASNALVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDNVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTAVPDRIIAGTPQNSSGSVITATVVDNNGFPVKGVTVSFTSRTKSAEMTNGGQAVTN
                     EQGKATVTYTNTRSSRETGARPDTIEASLENGSSTLSTSIQVDADASTAHLTSLYTLY
                     DTQLAGDDTTLYITVNDNYGNGVPLHQVTLSVSPSEGVTLSNNGINTTNHDGYLYASM
                     TATKAGVYQVTATLDNGDSMQHTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVRANFTLSDGGKAITDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSGQLVVNFTADTLTAQVNLNVTEDNFIANNIGMTKLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNSYGVSDTKPVT
                     LIADAGTAKLAGFTASSGSFTASTTEGATLTASVTDAYGNPLEGIKVNFRGPATTLSN
                     TSVETDAQGKAEILVTSTIAGTKVVTANLAIAPTEAAMRTLTVKADIDSATITSLEMP
                     EGQVIIREPIAVKAHVDDQFGNPVADQLVTFSAEPSSFNMVISQDTVSTNRQGIAEVT
                     MTPGRYGSYTVKASLANGSFYEKDLVVIDLRLTLTSSSQLIGVNDPSGATLTVRLTHA
                     NGAPLSHELVTFSVTPEGATLSSQTATTNTSGEAQVVLTSNKVGTYAVTASIHSGVII
                     ETQTTVKVTGNPSTAHVASFIADPSTIPANNSDISTLKATVEDGSGNLVEGVNVNFVL
                     KSGSATLTSLTAVTDQNGLATTSVRGAMTGSVTVSAETSYGGAQTVDITLVAGPADAS
                     LSVLKNNRSSLKGDFTESAELHLVLHDLSGHPINVSEGMEFVQSGTNVPYVQVSAIDY
                     SKNFSGEYKATVTGGGEGIATLIPVLNGVHQAGLNTTIEFISAEARPMTGTVSVNGAT
                     LPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSSTASWVDVDTSGKVTFKNVGDRN
                     AVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLSQAENYCSNQVGNGYTLPRADLL
                     SNGHMRREIGSLYGEWGDMGNYMNEADFYSMVYWSSNSAGAGQQYIVSLETGTQNTYQ
                     THEFFYGACYKQI"
     gene            complement(12929..13004)
                     /locus_tag="ACHK1J_003893"
     tRNA            complement(12929..13004)
                     /locus_tag="ACHK1J_003893"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12969..12971),aa:Asn,seq:gtt)
BASE COUNT         3173 a         3165 c         3190 g         3489 t
ORIGIN      
        1 ataaaactcc tatccgccta aggaagcaaa cagcctgctt tattgcaggc tatttgccag
       61 atgtcttact taatccttca catctatttt tgataagaag gttcagaact tattatcgga
      121 acggcggctc attaaaggtt cgtaatttac gtgaatgcag tcggtcgcct tctgcgcgca
      181 gcaaatcgat tgcccgaatg ccaatttgca ggtgttcgga aatagcgcct tcataaaaac
      241 ggttagcctg gccgggaagt ttaatctcgc catgcaacgg tttatctgaa acacacagta
      301 gtgtcccgta tggcacgcgg aaacgatatc cttgcgcggc aatggttgca ctttccatat
      361 caattgctac ggcccggctt aagttaaaac gaagtgcaga agctgagtaa cgtaattccc
      421 agttcctgtc atctgtggtt accacagtgc cagtacgtag ccgctgtttg acttcctcac
      481 cgggcctgcc gctcaccagc ttggtggcgt cataaagcgc acgttgcact tcagcaatgc
      541 ttggaatagg aatatcgggc ggcagaaccg catcaagaac gtgatcatcg cgtaaataag
      601 catgtgcaag tacataatcg ccaatggcct ggctttcacg taatccgcca cagtgaccaa
      661 tcatcaacca gacatccggg cgtagcactg ccagatgatc gcagatggtt ttagcatttg
      721 acggtcccac gccaatattc accagagtaa taccctgacc atcggcggta attaaatgcc
      781 atgctggcat ctggtgtttt ttccatgcaa ggtcggaaat ggcttcttct ggcgcttcgg
      841 tttcggcggt gatccagttc ccgccagcac aagaaagggc aatgtaggga ctatcaggat
      901 cgaggatctg gctgcatccc caacgaacga attcatccac ataacgtgtg tagttggtaa
      961 acaagacgaa cggctgaaaa tgttcaactg gcgtaccggt ataatggcgc aaccgcgcga
     1021 gggaaaaatc gacgcggcgc gcatcaaaat gcgatagcgg ggagaattca gttgggtgat
     1081 aaatgccgtc tgcagtttca tcgccaattt gcgccagttc tgttgtcggg aagtagcgag
     1141 ttaacccagc gctcattgag cgatcaagtg tcaattcaga gccatcgatg acataaggat
     1201 aagggatttc atgctgcgag ggttgcaccg agatatgcgc accataatcc tgatacagca
     1261 acgtaagttg ttcattaaga tacgaacgaa agagagtagg gcgagtaatc gtggtggtgt
     1321 agctgcctgc gtgagtaaaa cgaccaaatg cgcgcgtttt ggggggattg gttgtgccac
     1381 cgtcccaggt tacggtcagt gatggataga caaaaagacc ttgtttgcgg gcgttttcat
     1441 caggtaattc gccacttcta atatatttgc caatggcgtt gcgtaatgcg actacagatt
     1501 gctcatacag cgcgtcgagt ttatccagtg cctgagctgg ggtcagaccg gagcccttat
     1561 tattcatgtg tgtctcctgt tccatacaat taccgcacat ccgatagtat gtcacaggaa
     1621 tgtgaaacaa aagtgtaagg cgaagatcgg atgatcttcg ccagactatc aagcgcgttg
     1681 attgtctttc atcaacaaag cggtcattgc ggaaattaga catccagcca gcaaataaat
     1741 ggcgacgcta tgccagttcc cggcaaagta agtgatgagc gcagcggcaa taaaaggtgt
     1801 aaatccaccg ccaaccacac tggcaacctg ataaccgact ccagcaccac tatagcgata
     1861 actggcacca aacatttcgg taaacatcgg ttgttgcaca cacaccacca tgtcatgcgc
     1921 aatgtttgcc agcattatgg agaagaaaac tatccagaaa atagattgtg cttcaagcgc
     1981 cataaagaaa ggaaatgcgc tcaacgttcc gatcaacgcg cctgtgatat aaaccctgcg
     2041 gcgaccaaaa cgatcggcaa gccaggcaaa acagggaatt gtcaggcagc ttaatccacc
     2101 taccagcaaa ccaatattaa ggaaaagttc gcgcggtaac cccatattct gggttgaata
     2161 attaagtgca aaggcggtaa cgatgtacat cgtcagcaac tcgcacagtc gtagcgcaat
     2221 aatcttcagg aaagcaccgg gatgtcgtaa cagagcttcg ataaccggga tgcgtttttt
     2281 cgcggcagct tgataatgtt gctgttgttc aaattccgcg gactcctcca tgccattgcg
     2341 cacccacaat gctcccagta ccagtacgat gctaaacagg aaaggaatac gccagcccca
     2401 gcttaaaaac tgttcgtcag tcgtcatcat actgatcaat gaaaccagtc cggttgaaag
     2461 cagtaaacct acaccgtagc caacttgtac accgctactg taaaaggctt ttttattttt
     2521 tggtgcactt tcaacggaaa gcaacgccgc gcctccccat tcgccgccga cggcaaatcc
     2581 ctgaatggca cgcagcgtca ccagtaaaat aggtgcccac cacccaatgg tcgagaatga
     2641 aggaagaata ccaatcaagg ctgtcgcgat acccatcatc cagacggtca gcattaacat
     2701 gcgcttacgt cccagtcggt cgccaaagtg accgaaaatg acaccgccga gcggacggaa
     2761 aaggaatccg acgccaaagg tagcaaatgc ggcgagcgtt cccatcgccg ggcttacttg
     2821 cgggaaaaac tcgcgattaa acaccagtgc ggcggtgatg ccatagagta aaaaatcata
     2881 ccagtcgacg acggcaccag cgaaactgcc taacgcagct cgtcgggcgc gacttaacga
     2941 aagcgtccct tcatcgggac gggtggagat gagcgtggag tccataatga tcctgtttgt
     3001 actgtctttt tataattagt atggaaaagg taactcacat tgccgtcgat ctataaacga
     3061 accttatgag actacctcga cagagcgcat ctgcaaatga ttttatggct gattagcggc
     3121 aacatgaaaa attcgctgga agatgaaagc gcgggagttg ctggtgttgt ttaaggatta
     3181 agcagaggac ccacaggttc gagatatgaa acataaaagt ccgcatatgc acaaaatggc
     3241 accctgatga gtctgataga ctgcattgca ttataaccag tagggaggcg aggttaggta
     3301 tgtttttagc atcattgttg agacatattg catttagtta ctacgattat aaagcttata
     3361 atttcaatat tgaaaaaaca gattttgttg tcatccatat tcccgatcag attggcgatg
     3421 ctatggccat ctttcctgtt attcgggcgc ttgaattgca taaaattaag catctactaa
     3481 ttgttacgtc gacaattaat ttagaagtct ttaatgcgct caaacttgaa cagactaaat
     3541 taacattagt cacaatgact atgcaggatc acgcaacatt aaaagaaata aaagatttag
     3601 cgaagaacat aacacagcaa tacggtacgc cagatctttg tatagaggcg atgcgtaaaa
     3661 agaacctgaa aacgatgtta tttatcagtc agttgaaagc aaaaacgaat tttcaggttg
     3721 ttggtttaac catgaagtgc tattcccctg tgtgtaagaa cgcgtcccgg atggatcaga
     3781 atctccgggc tcccgtaccc atgacatggg catttatgat gcgtgaggcg ggttttccag
     3841 cagtcaggcc aatatatgaa ttgccactaa gtgaggatgt actcgatgag gtgcgcgagg
     3901 aaatgcgatc gttaggatct tacattgcgc ttaatttaga aggtagctcg caggaacgta
     3961 cattttcatt attgattgca gaaaatctaa tagcaaaaat tcaaagtgaa acagatatac
     4021 caatagtgat cgttcatgga ccaaaagggg aagataaagc cagggtatta gtggattgtt
     4081 ataataatgt ctaccgttta tccttatcac cctcgattaa acgttcagca gcaatcataa
     4141 aagatgctta tatcgcaata actcctgaca cctcaatatt acatatggca agtgcctata
     4201 atacccccgt tgttgcaatt tatgctgatt acaaaacgcg atggcccgca atggccgatg
     4261 tttcggagtc agtcgtcgtt gggcaaaaaa ttgacaatat aagtctggat gaattcgcaa
     4321 aggcattaaa aagtgttttg gcgagaattt gaggcagtac gtatgcatag taatgccgat
     4381 ctgttatgaa cggtggtgtc atcaatactc tgaggctggt caatcatcac agaaaaccag
     4441 cctttggatc aaagctgtac tcaccggata cgatcatagt cacaccgaac gcgaatctgg
     4501 cggtatcgca tttttattta ttcattttcg gtgagagaag agcaagctct atagagggaa
     4561 tgtaaaaaag agtgccccga atggagcact ctttaagaaa ttataacgaa aaattaaatc
     4621 tgtttataac acgccccata aaaaaattca tgcgtttgat aagtgttttg cgtccctgtt
     4681 tccagggaga caatgtattg ttggcctgcg cctgcagaat tagatgacca atataccatt
     4741 gaataaaaat cagcttcgtt catgtaattt cccatatctc cccattcacc atacagtgac
     4801 cctatttcgc gccgcatgtg tccattgctt aaaaggtccg cgcggggaag ggtgtagcca
     4861 ttaccgacct ggttgctgca gtagttttca gcctggctta attgcatgag gttattaccg
     4921 tgattcaccc accagccctt cacccgtact tgcgtttggt aaatagcgcc acctgatctt
     4981 ggggtcgccg tgattattac agcatttcta tctccgacat ttttgaatgt caccttaccg
     5041 gaagtatcaa catcaaccca ggaggcagtg cttgaaaacg cataatcagc cgccgttttt
     5101 cctggtgcaa agttgtcatt attcaactga taatacgccc cggtaaatcc ctgcgaaggg
     5161 aatgaagctg ccggtagggt cgcaccattg actgacactg taccggtcat tggtctggct
     5221 tccgcgctaa tgaattctat cgtggtattc agacctgcct ggtgaacacc attcaacacg
     5281 gggatcagcg ttgcgatacc ctctccgccg cctgtaacag tggctttgta ctcgccggag
     5341 aaatttttgc tgtagtcaat tgcactaact tgcacatagg gcacgttggt acctgattgc
     5401 acaaattcca tcccttcaga aacattgatc ggatggcctg atagatcgtg cagaacaaga
     5461 tgtagttcag cactttcggt aaagtcccct ttcagtgatg accggttgtt cttaaggacg
     5521 gacagcgaag cgtctgccgg gcctgccacc agcgttatat ctactgtttg cgctccacca
     5581 tagctcgttt ctgcgcttac cgtgacgctc cccgtcatag ctcctctaac gctcgttgtc
     5641 gcgagtccgt tttgatcggt caccgctgtt aatgacgtca atgtggcaga gccgcttttt
     5701 aagacgaagt ttacattgac cccttcgacc aggttaccac tgccatcctc aacagtagcc
     5761 tttagcgtgc tgatatcact attgttggcc gggatagtcg acggatcagc gataaaacta
     5821 gcaacatgtg cggtgcttgg gttgccagta accttcacgg ttgtctgtgt ctcaataatt
     5881 acgccgctat ggatcgatgc tgtcaccgca tatgtaccga ctttattgct ggtcagaacg
     5941 acttgtgcct cacctgaagt gttggttgtc gctgtttggc tacttaacgt tgcaccttcg
     6001 ggcgttacgc taaaggtaac gagttcatgg ctcaatggag ccccgtttgc atgagttaac
     6061 cttacagtca gagttgctcc cgatgggtca ttgacgccga taagttgact tgatgacgtg
     6121 agtgttagtc ttagatcgat taccaccagg tccttctcat aaaaggagcc attcgccagg
     6181 gatgctttca cagtatacga accgtatctt ccaggcgtca tggtgacctc agctataccc
     6241 tgcctattcg tagaaaccgt atcctgacta atgaccatgt taaaacttga cggctctgca
     6301 ctgaatgtca caagttggtc agcaactgga ttgccaaact ggtcgtcgac gtgtgcttta
     6361 accgctatgg gttctctgat tattacctga ccttcaggca tttccagact ggtgatcgtc
     6421 gcagaatcaa tatctgcttt gacagtcagc gttcgcatgg cggcttcggt aggtgcgatt
     6481 gccaaattag ctgtaactac tttcgttcca gcaatggtgc ttgtcacaag gatttctgcc
     6541 ttaccctgag catccgtttc gacactggtg ttactcagcg tggtggcggg gccgcggaaa
     6601 ttgaccttga taccttctag cgggttgccg taagcgtcag tgacgcttgc cgtcagggtc
     6661 gcgccctcgg ttgtgctcgc ggtaaatgag cccgatgatg cggtaaaacc agccagttta
     6721 gcggtaccag catcggcaat caaagtcacc ggcttcgtat cactgacacc gtaactgttc
     6781 acgctaactg tcacgggata ggtgccggat tttgtaccgc tcagtgtaac ttcagccttg
     6841 ccgttgatgt ctgtaatggc ggaaccgcct tgtccgagag tgaagcttgc gctcacatct
     6901 gccggtagtg tgaatgttac cgcctcattg gctaacgggt tgccgtttcc atcagtcact
     6961 gttgcctgaa gtttggtcat cccaatgtta ttggcgatga aattgtcctc ggtaacgtta
     7021 agattaacct gcgcagtgag cgtatccgca gtaaagttca ccaccagttg tccgctctta
     7081 ccgccagcca tcgatgctgt aacagtatga gcgcctgctt ttgtaccttt cagcgtgact
     7141 ttcgctttgc cttcagtatc agtaatcgct ttaccgccat cgctcagtgt gaagttcgcc
     7201 ctcacatctt ccggcagagt aaatgttacc tcagtgttgg ctatcgcatt gccctctgta
     7261 tcagcgactg ttgctgttag tgtcgtgaga tcgttattgt cggcaatcac cggatccttc
     7321 gaggctgcca gcgtgatttc agcattcgcg acgttcggca cataggtcac tgtgtgttgc
     7381 atcgaatcgc cattgtcgag ggttgccgtc acttgataaa ccccggcttt tgtagcggtc
     7441 atgcttgcgt ataaatagcc gtcgtggttg gtagtattta taccgttatt actgagggtc
     7501 acgccttcac tcggcgaaac gctgagggtt acctgatgca ggggtacacc attgccgtaa
     7561 ttatcattca ccgtaatgta cagagtggta tcgtcgcctg ccaattgtgt gtcataaagt
     7621 gtatatagcg aggtgagatg tgccgtagac gcatcagcgt cgacttgaat ggacgtgcta
     7681 agcgtggagc taccattttc caggctagct tcaatggtat ccggtctcgc tcctgtttct
     7741 ctcgaggagc gggtattggt ataagtgacg gtagctttac cctgttcgtt cgtcacggct
     7801 tggccgccgt tcgtcatttc ggcagatttt gttctgctgg taaagctcac agtcacacct
     7861 tttaccggaa agccattatt atcaacgact gtggcggtaa tgacgctgcc ggagctgttc
     7921 tgcggggtac cggcgattat gcggtctggc acagccgtca actcgataat ttttgccgcc
     7981 gctgtgtcgc caataaaatg cacagttttg ttgtcgctgg caccattatt agccagtgat
     8041 gcagtgaccg tctgctcacc aaaggcaacg cctgcgagag tggcctgcgc gatgccagac
     8101 tcattggtat tactcactcc cggggtcagg gtgagtcctg aagaggctga gctgaatgtt
     8161 accggaagat tgttcacctc attgtcgaac tgatctttaa ccgttgcagt tagcgttgcg
     8221 ttatcgacgc cattacctgt gatctcatct tttgacatct gcaggacaac ctgagccgag
     8281 gttttgtccg ccacaaacgt taccggctgc gaatcactgg tattgttatt acccagcgtt
     8341 gcggtaaccg tatgcgtgcc cgctttttta cctttcagcg tgacatgcgc ttccccattg
     8401 gcctgggtaa tggcaatgcc gttattttca agggtaaagt ttgctgccac gtcctgtggc
     8461 atggtgaagt ttaccgttat ccccgctacc ggatgattcg atgggtcttt caccgtcgcc
     8521 gtcagctgca cattgtcgct gttattcgtc accacctgtt gagcagggat gttcagggtg
     8581 acctgcgcgt tgctggcatc gggcgcaata ttgactgtcg tcgtatagcc attgccgtta
     8641 agcagtgtgg cttcgactgt atgaacaccc aaaaccgtgc ccttaagggt aacttcagca
     8701 acaccactgt cgttagtatt gcttgtgctc tgactaagtt gagtatctgc cgggttggta
     8761 ctgaaggtga cctgtagatc ttttactgcg ttatcaaaag gatctttcac tgttgctgtc
     8821 agagtcgtct catccacgcc attcccaatg atttccgctt tcgatgtttg cagaacgaca
     8881 actgcgctgt ccttatccgc cacgaatgtg actggttgcg catcgctggc attattgtta
     8941 cccagcgttg cggtaaccgt atgcgtgccc gcttttttgc ctttgagggt gacgtgcgct
     9001 tcgccattgg cctgagtgat ggcaataccg ttattttcaa gggtaaagtt tgccgccacg
     9061 tcctgtggca tggtgaagtt caccgttatt cccgccaccg gatgattcga cggatctttc
     9121 accgtcgccg tcagctgcac gctgtcgctg ttattcgtca ccacctgttg agccgggatg
     9181 ttcagggtga ccagcgcgtt gctggcatcg ggcgcaatat tgaccatctt cgtatcgttg
     9241 ttgccgttag gcagtgtggc ttcggctgtg taaaccccca aaaccgtgcc cttaagggta
     9301 acttcagcaa taccgttctc attagtatta cgcgcgttca gactcagttg cgtgtctgcg
     9361 ggggaggtgc ggaagactac tgacagattt ttaaccacgt tatcaaaagg atctttcact
     9421 gttgctgtca gagtcgtctc atccacgcca ttcccaatga tttccgcttt cgatgtttgc
     9481 agaacgacaa ccgctctgtc tttatccgcc acaaacgtca ttggctgtgt atcgctgaca
     9541 ttgctgttag ccagacgagc cgtgatcata tgcgtgcccg ctaacgtgcc ggtcagggag
     9601 gcgattgcaa tcccgttgct atccgtcatg ccttttccgc tgttgctaat cgagaaccga
     9661 cttgcgacgt cgtttggcac agagaaagtg atttctttat cttttagtgg gttgccattt
     9721 ttatcctgca aggttgccgt cagctgttga ggcgctgtgt cggtgacggt gatctcgcct
     9781 gggggcacac tgagggtcag ggctgcagta ctttgatcac cgataaaaat cacctgttga
     9841 ttagcctgag aaccagagct cacagaggcc gtaaccgtat aatcaccatt tttcaaactg
     9901 gtcagcgtag ctgtggcgat cccgtcgtgg ctattcactt cggtttggct cagtttcgct
     9961 gctgctgaat taacattgaa ggtgaccttg acgtcattga gcaggttgcc ttttgcatcc
    10021 cggactgtcg cggtcattgt ggcgctgtca ttgccgtcag cgaccacttc atttttcgac
    10081 ttctgcagat caacctgcgc ggtactcgag tcgccgacaa aactgacaat taatgtttgt
    10141 ttcacgccat tttcaagggt gacttcaacc gtgttgtctt cctgcttact acttttcaga
    10201 tcaaaagtcg ccagaccatt aacatccgtt tttgcggtgt tttgattgtt aaaggaagtt
    10261 gccgatccgc ttaataccgc aaacgtgacg gtatgatcat tgattgggtt gcttccttca
    10321 tcagcgacat tgaccgagac ggtgtttgct gcattctcat tggcgagcac accattattg
    10381 ctggcagata atgtcgcaat tttcgctgac tgcgggttgg cgtcgatgat aaatccagcg
    10441 gtatgcaaat cttcattcca gttttgcatt aacagcttcg cagtaagccc actgcctttg
    10501 gtataggcgg tataggttgc cttatagacg ccatctgcgg tttctttcca gtctgtagtg
    10561 acaccaggtt tcacattgtc gatgctgact gcggtattca gttgctgttt ttgctcctta
    10621 acaggtttgt cattttcatc tctcagttct accgtcacct cgataggatt cccggagaga
    10681 taacggtcct tatcaatctt aattgacgag tgagtccggg atgacgaaac agaaatgata
    10741 ttcaccacgg cgggggcttt agccgcatcc acaccgttca gctgtggcat cagcgtcagc
    10801 gtgccagaca tcgctcctgt ggtcaggact tgggtatagc ttccgtcacc attatctttc
    10861 cagtccgaca gggtgatgtc ctgaacacct tcgtgacgcg tcgagagcac cagcccgata
    10921 acaggattac ctgctgcatc atgagcaata aaagtcagtg tggcgcttga atgggaatcc
    10981 gcgctcaacg tctgggtact taacgatacc gaggaatctt tctggcttag cgtaggtgcc
    11041 tgaacgacta ccatgctctg ttcacgattc gaaaaattgc ctttgacatc ttcagcggtg
    11101 acttcaatcg gccaggtgtt atcggtttct ggcgtactgg tgaaccggta cgccggcagg
    11161 gtaaccagaa tatctttacc cgttgtaacc actttaccac cggcagcttt cagagcggtg
    11221 gcttcgacgt tatagccttt cagggcatat ttggtttgta gcgacgaaac cagtgatttc
    11281 acttctcctg acttccctgt cacggggtct gtcagggtca ggcggaccag ttcttttttg
    11341 cgatattcca gaacgatgtt gttgttgcga tccaccagat cataacggct gcctgcaagg
    11401 ctacgccgtg cagcgacttc attcgggtca agctgtttct gcattgcgct gccaggttgc
    11461 caggtaaaat cgacggcaaa tcgggtgtca ttttcgccct gtttaccctg gcgttgctcc
    11521 gcgctaaagg tcatcagcgg gaagggggta tagtttagtc cagcggttat ggcatgagga
    11581 ttactttgcc gatcgtcttt atcgaacagg gccacttcat cgccataata ctgttcatag
    11641 accagtttac cgccaaggtg cggccaggcg ggtaaccagc cttctgcgcg tacatcccag
    11701 ccattggccg ggcgtgcttc ataatcgttg tccagttcag gtgcgctgcg ccagtttgtc
    11761 agtcgcaaat agccgttact gcttaattta agatagtcgc gccagtactc cgcgccaatg
    11821 ccggcgcggg agtggtaacg gctaagatcg tggtcgaaaa agaagttgat gcccgacatc
    11881 catgtgggag tgaaatgacg ccaacccaag ccgttgttaa tctgcgtacg ctcgtcagta
    11941 cgatggagag tatgctgact gaaaaagaga ttatcaggtg tttcatacca cggatggaga
    12001 aaatcgaact gggagttctt caggctaaaa tcttcatcca cgcccagcgt gattcttgcg
    12061 gtaccgaagc ggcttaacca gtctgtcatt gcgcctgaag cctgagaaga ggcccatcca
    12121 cgcgccatat ttgccgcttg ctcgctgttc atatcttcgg cgagcagaga tccgatttgc
    12181 tgtgaagtac tggctatctg ttgctcaagg ttgccactgc tattacccgg tggcggggtt
    12241 aaattatttt cactaacttg tgccgggaca tccagttcat caccctggcg gacattatca
    12301 aaacctcgag caaacgtacg aaactggttg agtttgcgta actcagccac cgaaataccg
    12361 aaacgttcgg caacgctttg ggccgattcc agcgctccaa gggtgtaggg caccgtattg
    12421 gcatttgcaa tggcaatttg tgcaggaact ggttgttggg ttgcggcgtt taccacacct
    12481 tgtgctgccg cagccatagg gaacacaagt tgagttacca ggcagatacc cgcagttaag
    12541 cggcgtagtt tgatacccat cccgcataag atttgtcggt catttatttc ttctccactt
    12601 ctcttcttcg tagccatgct taccttccct gaacgagcta acataaatgc gccctttcta
    12661 gcacagcgaa aaaatgtaaa tcctgatagc aatatgattg aaaattaata acttttcgtt
    12721 aggcagtttt gggtgtgagt tgcaagaggg gagactactg aattactcaa gttttataat
    12781 cgaggggaaa atggtgatgg cgccatagca aaacgccctc aaccataaag gtcgagggcg
    12841 cttaagatgt caaaacccgc tatccgttaa aaaacaatgt tcaactaagg tcagtgacat
    12901 tgcgctaaaa aagcgaattt ttagaatttg gctcctctga ctggactcga accagtgaca
    12961 tacggattaa cagtccgccg ttctaccgac tgaactacag aggaatcgtg tgaacgg
//
LOCUS       DAZGBC010000142        13004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGBC010000142 DAZGBC010000000
VERSION     DAZGBC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.143"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            256..1098
                     /locus_tag="ACHK1J_003894"
     CDS             256..1098
                     /locus_tag="ACHK1J_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO7913103.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNANSEVSILKKIKIPLQETPTNCGFEGLAYSPQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1166..2344
                     /gene="yjiJ"
                     /locus_tag="ACHK1J_003895"
     CDS             1166..2344
                     /gene="yjiJ"
                     /locus_tag="ACHK1J_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7913104.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            2555..7795
                     /gene="iatD"
                     /locus_tag="ACHK1J_003896"
                     /pseudo
     CDS             2555..7795
                     /gene="iatD"
                     /locus_tag="ACHK1J_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(7919..8503)
                     /gene="kptA"
                     /locus_tag="ACHK1J_003897"
     CDS             complement(7919..8503)
                     /gene="kptA"
                     /locus_tag="ACHK1J_003897"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO7913105.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            8753..9436
                     /gene="yjiH"
                     /locus_tag="ACHK1J_003898"
     CDS             8753..9436
                     /gene="yjiH"
                     /locus_tag="ACHK1J_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO7913106.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9433..9894
                     /gene="yjiG"
                     /locus_tag="ACHK1J_003899"
     CDS             9433..9894
                     /gene="yjiG"
                     /locus_tag="ACHK1J_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO7913107.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLTTAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9907..11079
                     /gene="iadA"
                     /locus_tag="ACHK1J_003900"
     CDS             9907..11079
                     /gene="iadA"
                     /locus_tag="ACHK1J_003900"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7913108.1"
                     /translation="MIDYNAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11144..12055
                     /gene="hypT"
                     /locus_tag="ACHK1J_003901"
                     /gene_synonym="qseD"
     CDS             11144..12055
                     /gene="hypT"
                     /locus_tag="ACHK1J_003901"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO7913109.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12048..12440)
                     /gene="iraD"
                     /locus_tag="ACHK1J_003902"
     CDS             complement(12048..12440)
                     /gene="iraD"
                     /locus_tag="ACHK1J_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO7913110.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLVISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12437..12520)
                     /gene="idlP"
                     /locus_tag="ACHK1J_003903"
     CDS             complement(12437..12520)
                     /gene="idlP"
                     /locus_tag="ACHK1J_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO7913111.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(12636..12779)
                     /gene="ytiD"
                     /locus_tag="ACHK1J_003904"
     CDS             complement(12636..12779)
                     /gene="ytiD"
                     /locus_tag="ACHK1J_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO7913112.1"
                     /translation="MADYAEINSFPPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         3379 a         3248 c         3146 g         3231 t
ORIGIN      
        1 acccaaacag agcaggtatg gcagaggata taaattaaca tttaaacgtt aaccgcctca
       61 tctatcattc atggatgagg cgcatttatc agtcgatgta agctgaaaat gttctgccat
      121 ccagagacaa ccgccgcccc ctgcggcgtt tactatttca tcgcccgcgt taagaattca
      181 ttaagcttca tccgtatact ccttatctgt cgcactgaat ttgacgagaa aaaagcaata
      241 ataaggagca ataagttgaa aaagcgcatc acctttatcg tttttctttg cgccatcgtg
      301 gcggcaagcc tgttttttgt gcaatcctgc gtccgtaaaa gccagcatgt cgcagggttt
      361 cagaattatc aggccactat cgatggtaaa gagattacag gagtaactaa aaatatctcc
      421 tcactgacct ggtcagcgca gagcaatacc ttatttagca ctatcaacaa acctgcaacc
      481 atcgttgaaa tgacgaccga gggcgacctg atccgcacca ttccgctcga ttttgttaag
      541 gatctcgaga cgattgaata cattggtgat aataaatttg tcatcagtga tgaacgtgat
      601 tacgccattt atgtgatttc acttaacgct aactcggaag tgagcatcct caaaaaaatt
      661 aaaatcccat tgcaggaaac gcctactaac tgtggctttg aaggactggc ttactcccct
      721 caggaccata ccttctggtt ttttaaagag aaaaatccga tagaggtcta caaagtaacc
      781 gggctattgc gtagcgatga gttgcatatc agcaaagata aagcgttgca gcgtcagttt
      841 actttagacg atgtttctgg cgcggaattt aacccacaga aaaatacgtt gctggtgttg
      901 tcacatgagt cacgcgccct gcaggaagtg accgtgagag gtgacgtgat cggcgaaatg
      961 tcgttaacca aaggtaagta tggcctttca cacaacatca agcaggcgga agggatagcg
     1021 atggatgata gcggcaacat ctatatcgtt ggcgaaccga atctttttta tcgctttacg
     1081 tcgacaaaat cacgataaca gtctggcagg ttctcctttt ttgttattaa cttgttaact
     1141 caaaataata caaaccaggc agaacatgaa ttcgacatcg catcccgtag aacgtttttc
     1201 tttcagcacc gcgttattcg ggatgctggt tctgacctta ggtatgggtt taggccgctt
     1261 tctctatacg ccgatgctgc cagtcatgct ggcggaaggc gagttttcct ttagcgaact
     1321 ctcatggatc gccagtggta actatgccgg gtatctggcg gggagcctgc tgttttcatt
     1381 cggcgcgttt catttaccct cacgcctgcg cccgttcctg ttagcttccg ccctcgcaac
     1441 cggattatta atcctcgcga tggcgtggct gccgccgttt cttctggttt ttatcattcg
     1501 ctttctggcg ggagtcgcca gcgccggaat gttgattttc ggctcaacgc tcatcatgca
     1561 acatacccgc catccctttg tccttgcggc gctattttct ggtgttggcg tcgggattgc
     1621 tctgggcaat gaatatgtgc tggcaggcct gcattttgcc ctctcttcac aaacgttgtg
     1681 gcaaggtgcc ggagcacttt ctgccattat attgcttgct ctggcgctgc tcatcccgtc
     1741 gaataaacac gttatcccgc cagcgccatt ggcaaaaatc gcgcaacaac ccatgagctg
     1801 gtggttactg gcgattctgt atggtctggc gggttttggt tatatcatcg tcgccaccta
     1861 cctgccgctc atggcgaaag acgcgggcca gcctgtgttg acggctcacc tctggacact
     1921 ggttggcttg tcgattgtcc caggttgctt tggctggctg tgggcagcca aacggtgggg
     1981 agcattacct tgcctgaccg cgaatttgct ggtgcaggcg atctgcgtgc tgttaaccct
     2041 cgccagcagc tctcctttat tactcatcat cagcagtatt ggttttggcg gcacctttat
     2101 gggaacgacc tcgctggtga tgaccatcgc ccgccagctt agcgtgccgg gaaatcttaa
     2161 ccttttgggc tttgtgacac tcatttatgg tatcgggcaa attcttggcc cggcgctgac
     2221 cagtatgctc ggcaacggaa cgtcggcgct cgccagtgcc acactctgcg gcgcagcggc
     2281 gctatttatc gcagcattaa tctgcgggat gcaaatattc aaattgcata cgaattattc
     2341 ttaagtaatt taacgaattt aacttttcag tacatcacgt aaaataatta aaacatcctt
     2401 tttctttgga ggtactcagc caccggcgtt attagtcctg gtttagcaat tttacggcta
     2461 attcattatt tctggtcaca ttatagtcag gatcgcttta tatctccgtg ctagaaaaaa
     2521 caagtttaat tctgtttttt cacggaaggt cactatggct tcgacaaatg cgcatcaaat
     2581 aaaaaataat gatcaaaact ctttatgtgg tctgggtgac aaaatacgcc gcctgaccgc
     2641 cggagtttgt ttatttacac aaattttttt ccccgtcatg gcgacggcac aaaatgtggt
     2701 acatgctaaa cctcagacga cagtttcatc tgccccccct ctaatagaaa ataatacggt
     2761 gccttacacg cttggtgcgc tggaatcagc gcaaagcgtt gctgatcgat tcggtatttc
     2821 actggaggag cttcgtcgtc ttaatcagtt ccgtactttt gctcgcggct ttgataacgt
     2881 gcgccagggt gaggaactgg atgttccggc aacaacctcg cagaaaagcc atgagcaaca
     2941 aaatgccata ccacctgcga atggcgaaaa cacgctggag aatcaaatag ccagcacctc
     3001 gcagcgagtt ggcactctgc tttcacaaga tatgaatagt gagcaggcca gcggcatggc
     3061 gcgtggttgg gcgtcttcag aagcctcagg cgcgatgact gattggttaa ataactttgg
     3121 tactgcgaga atctctctgg gtgtggatga agattttagc ctgaaaaatt cgcaattcga
     3181 cttcctgcat ccgtggtatg acacacctga ttatctgctc ttcagccagc atacccttca
     3241 ccgaacagac gatcgtaccc agatcaacac cggtttgggc tggcgtcatt tcacctccag
     3301 ctggatgtca ggcatcaacc tgttttttga ccacgacctg agccgctatc actcccgcgc
     3361 agggcttggc gcagaatact ggcgtgatta tctgaagttg agcagcaacg cttatatcgg
     3421 cctgaccggc tggcgtagcg caccagaatt ggataacgac ttcgaagccc gcccggccaa
     3481 cggctgggat ttacgcgcgg aaggctggtt acctgcctgg ccacaactgg ggggaaaact
     3541 ggtctatgaa caatactatg gcgatgaagt ggcgctgttt gacaagaatg atcgtcaaag
     3601 taacccccat gctattaccg caggcctcaa ctataccccc ttcccgcttc tgactctcag
     3661 tgcggaacag cgtcagggga agcaaggtga aaatgacaca cgttttgccg ttgatctgac
     3721 ctggcaaccc agcagttcaa tgcagaaaca gcttaacccg gacgaagtgg ccggacggcg
     3781 cagtctggcc ggtagccgtt atgacctgat tgatcgcaac aacaacatcg ttctggaata
     3841 tcgcaagaaa gagctgattc gcctgagtct gctggatcca gtgaaaggga agtctggaga
     3901 aataaaaccg ctggtttcct cgctacagac caaatatgcc ctgaaaggct ataacatcga
     3961 agccgctgcg ctggaagctg ccggaggtaa agtcagcacg tctggaaaag atatcacggt
     4021 cacgctgcca ggttaccgct tcactaacac cccagaaacc gataatacat ggtcgataga
     4081 cgttaccgcc gaggatgtaa aaggtaacct gtctcggcat gaacaaagca tggtagttat
     4141 tcaggctccg acattaagcc agaaagattc tctattatcc gttaatccgc taaccgtggc
     4201 tgcagataaa aaatcgacga ccacattgac cgttactgcg cacgattccg acggaactcc
     4261 ggtgccgggg ctggcgctgc aaacccgcag tgaaggcgtt caggatatca ccctgtctga
     4321 ctggacagat aacggtgatg gtagttacat acagatgctg accgccggaa cgacatcagg
     4381 ttcagtaaca ctgacgccgc aaattaacgg tgagagtgcg gtaaaagaat ccatcgtcgt
     4441 taatatcgtc cctgttgtct catcccgcga ccattcatca ataacaattg ataacgtatc
     4501 gtattatgcc ggagacgaca tcaaggttag ggtggaactg aaagacgaaa gtaatcaacc
     4561 ggttgcatat caaaaagagg aattggtaaa agccgttact gtcgaaaaca gcaaacctgg
     4621 caccacgatt gtctggcacg aagagcagcc gggcgtttat accgcgaatt atccggccca
     4681 taagcaaggg actgcactaa gggcacaact tagccttcac aactggaatg ctccactgca
     4741 atcgcatatt tataacattg aggcaaacca gaataaggct cgcgtcgcca cattatcagc
     4801 gacaaataat gacgtttacg ccgataaaaa gacatttaat accctcacta tcaacgtcac
     4861 tgatgagagt gataatcccc tgacaaatca tcaggtcacc tttaagaatg aaaaaggaag
     4921 cgcggaattt gtcgaaccgc cgcagcaaaa tacggatgca tatggtgttg ccacaatcaa
     4981 catggtaagt caggttgcgg aagaaaatac gattagcgcc acgctgccaa atggtttctc
     5041 acaacggata attgcgaaat ttgttagcga ttcgagtacg ccaaaattca aacaactggt
     5101 tgccgatcca gataccatta ttgctggcaa cagccagggc agtactctga ccgccaccgt
     5161 cacagacttt cataacaacc cgttaaaaga tatgaaagtg aattttgtgg cacctggtgg
     5221 ctcgcaactg gacaacacgt ccgccacaac agaccagtcc ggtattgtgc gggtgcacct
     5281 gaccagttca aaagctggta gctattccgt cgatgcctcg cttgaggtgg ataaaaatat
     5341 tcaccagtcg gtcacgatca ccgtggtccc aaacagggaa caatcggtaa tgaccttgaa
     5401 tgccgggtcg ggcagtgcga tcgctaacaa tacaaatacc gttaccctga ctgccagtgt
     5461 gaaagatgtt tatggacacc cgttgccgga tgaggatgtg aaatttacct tgccagcctc
     5521 catgaccggg aacttcacgc taagtagtga aaccgcccgc accgatgcaa acggtgatgc
     5581 cgtggtcaca ttgcgaggca caaaagcggg tgagtttaca gttacggcga cgctgaccag
     5641 aaataatacc gttgcttatc agcaagtcac ttttattggg gatacaaaca gtgcgcagct
     5701 ccagccgctg actgcctcat taaattccat tgttgcgggt aacagtacgg ggagtaccct
     5761 gacggcaacg atcctggacg cttaccaaaa tccgcttaaa gaccagtagg tcacattcca
     5821 gagtaacgat gtcactctaa gcggaacaga agtcaccacc aatacgctag gtcaggcgac
     5881 ggtaacaatg accagcaata ttgccgggca acataacgtc gtggtgagcc ggaaagcgca
     5941 agcctccgat aataaaacgt ttagtttatc agtgctcccg gatgaaagtt cggcgaaggt
     6001 aataagtata accggagccg aaaaaacgat aacggttggc gaaaacatca cgctacggat
     6061 actcgtccag gacgcgttta acaatgtaat agcgggtcaa cgcgtcagat taagtgcgca
     6121 gccaacagct aacattacga taggcgatac ggcttacacc gataataacg gttatgcgta
     6181 cgttaacctt attagcaccc agcctggggt ttatcaggtg acggcaacgc tggacaataa
     6241 cagtagtagt aaggttgacg tgaatgtggc aaatggcaaa ctcgagttaa catcatcgaa
     6301 accagaaacc acggtccata atagtgaggg tattacgctt accgcaacgg cgagaaatgc
     6361 gcggggtgaa ttgatgccag ggcaaattat cacctttagc gtaacgcctg aaggtgcaac
     6421 gctaagcaat acaggggaag tccttactga ccagtcaggt caggccaaag tgacgctgac
     6481 cagtgacaaa gtgaatgtct ataccgttac ggccataatg ggcaaagatg ttcccgttca
     6541 gagccaggta acggttgcgg ttaaggcaga tgctaaaacg gcacatgttg tgagcgtcgt
     6601 ggcttctcct gacaccatca ccgccgacgg catcgatagc agcaccatca cttcacgagt
     6661 agaagatgat tacggattcc cggttgaagg tgtcgatgtt agtcatggct tagacaccaa
     6721 aggcagcccg gtagttaata ttccaactac gcgtaccgat cagtccgggc aagtcacggc
     6781 gacaataacc agtacattgg cagaaacctt aacagtcaat gtgcaagttc ctggtacagc
     6841 caaccaatcc gcaaccatta cattggttgc cggcacggcc gatgaaagta agtcaatttt
     6901 gaaatccgat gttggcactc tgaaggctga ctaccagcag agcgcaaaac ttacgctaac
     6961 attgcaagac aagtacggta acccgatagt gacgtctgat catctggaat ttgtccagtc
     7021 aggccccttc gtgaactttc tcaagttgag cgatattgat tacagccaaa gaaattatgg
     7081 cgagtacacc gtgactgtca ctggcggaaa agaggggaca gcgaccctca ttcccatgct
     7141 gaacggggtt catcaggcaa acttaagcat atcgctgaat ctcatccaat cgataaaaga
     7201 aatgtccggt catgtcactg caaacaacca taccttctcc acggctaaat tcccgagcga
     7261 aggctttgca ggggcgtatt acacactcaa caatgataac tttgaagcgg gtaaaaccgt
     7321 tgatgattat atgttttcaa gttcacaggg ttgggtgtct gtcgatgctt caggtaaagt
     7381 ttctttcgca aatatcggcg atcaaacgtc agtcacaata agcgctgttc cccgacaagg
     7441 aggtacaacc taccagacct taattaagct gaaaggctgg tgggtgaata atggaaatca
     7501 taccaatatc tggctagctg ccaatgcgct ctgtcatgct aaaaatgatg gatataatct
     7561 tcctggcatc acacatttga cgtctggcga aaacaaacgc acgcagggat cactgtatgg
     7621 tgaatggggg aacgttggag cgttttccag taattcgcaa tttacaccgg gagcttactg
     7681 gacaagtgaa cctgatgatt acagtcggca ctactatgtg cagatgctaa ccggtatgac
     7741 cggaagcgac gctgattcca gcccccaact gaccgcctgc cgtaaatcac tttaatttca
     7801 gcagttgtta ttaaaggata cccttttacg agggtatcct ttcagactga taacaaacgc
     7861 tacattgccg gatgtgctgc gcttatcagg cctatatagc ccctgcaata tattgaattt
     7921 attgtttttg taccccagtc ggcataaacg accactcaag aaactcaaca gcaaccgttg
     7981 atgtcagcca gaccccgttt tccgcttgcc agaaagggat gcctcgtttc gccatctcct
     8041 gtgctttgac ggttaagata accggcgatc cgtgtcttgc tcccactttg cgtgccgttg
     8101 cttcatcagc agagagatgc acgtaatgac gatcccctgc aatcagcccc tgttttttta
     8161 tctcgtcaag gaaacggctt gccgtaccgt ggtagagaaa ctgtggcggt gttttttcgg
     8221 caaaggaaat agcgacctgc gaggtcgaat gcccctgaac cgcgcggatg cacctaccat
     8281 cactggaata actaaaacgc tttttatcgc tggtggcgac cacggtatcc agcaatgcac
     8341 gggttaatcg cttgcccgct ttttgggcac aaagtatgag cttatcgata tccgcccatc
     8401 cttcacggtc cagtacgata ccaatcgcct ccggtttgtg tcggaggaca aaacttaaaa
     8461 acttgctcgt atccgccagc tctttttcgt tgtactttgc catcgcgttt tcgtccctgg
     8521 attttctgtt tttatttacc aatgtcattg tcactgggtt tatgaatttt ctgcaactca
     8581 atcctgcgta gaaccttttc cacacagcgt aatttttccg ctatgcaaaa aacgcatagc
     8641 cagaaacata cttgcatttt ccccataccc acatggctga taaggttttt ccgctccagg
     8701 aattttcctg gctcaagcaa cacacaaaca caaaaaaaca atggggattg ttatgacaca
     8761 acaaggggat gctgtagccg gggagctggc gacagaaaaa gtgggcatta aagggtatct
     8821 ggcttttttt ctcaccataa tctttttttc cggtgttttt tccggaacag acagctggtg
     8881 gcgcgttttt gatttttccg ttctgaacgg atcgtttggg caactgcctg gtgcgaacgg
     8941 agccaccacc tcatttcgcg gcgcgggtgg tgcgggggct aaagatggct ttctctttgc
     9001 gctggagctg gcaccgtcag tcattctctc tctggggatt atttcaatta ccgatggtct
     9061 tggtggatta cgcgccgcgc agcagttaat gacacctgtg ctaaaaccgt tgttgggcat
     9121 tccagggatc tgctcgctgg cgctgatcgc taacttgcaa aacactgatg ccgccgccgg
     9181 tatgaccaaa gagctggcgc aggaaggtga aattaccgaa cgcgataaag tgattttcgc
     9241 cgcatatcaa accagcggta gcgccatcat caccaactac ttctcttccg gcgtcgcggt
     9301 gtttgccttt ctgggcacgt ccgtaatcgt acctttagcc gtcatcctgg tgtttaaatt
     9361 tgtcggagcc aacattttgc gcgtctggct taatttcgaa gaacgtcgca acccaacgca
     9421 aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca tggatatgtt tatcgatggc
     9481 gcgcgccgtg gctttactat cgccacgacc aacctgctgc caaacgtggt gatggcgttt
     9541 gtcatcattc aggcgctgaa aataaccggc ctgctcgact gggtggggca tatctgtgaa
     9601 cccgtaatgg cgctgtgggg attacccggt gaagcggcaa ccgtcctttt agccgcatta
     9661 atgagtatgg gcggcgcagt cggtgttgcc gccagtctga caaccgctgg cgcattaacc
     9721 ggtcacgatg tcaccgtttt actgcccgcg atgtatttaa tgggcaaccc ggtgcaaaat
     9781 gtcggtcgct gcctggggac agccgaagtg aatgccaaat attacccgca tatcattacg
     9841 gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc agcttattgt ttaacaagga
     9901 gttaccatga ttgattataa cgcagccggt tttaccctgc tgcagggagc gcatttgtat
     9961 gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta acggcaaaat tatcgccgtt
    10021 gccagcaata tcccttctga cattgtaccg aactgcacgg ttgtcgatct cagtgggcag
    10081 atcctctgcc caggttttat tgatcaacac gtccatttga ttggcggtgg cggcgaagca
    10141 ggtcccacga cgcgcacgcc ggaagtggcg ctaagtcgcc tgacggaagc gggcgtcacg
    10201 tcagtggttg gtctgctggg caccgactct atctctcgcc acccggaatc cctgctcgcc
    10261 aagacccgtg cgctcaatga agaaggcatc agcgcctgga tgctgaccgg cgcttatcat
    10321 gtcccttccc gcactattac gggttccgtg gaaaaagacg tggcgattat cgatcgtgtg
    10381 attggcgtga aatgcgccat ctctgatcac cgttctgccg caccggacgt ttatcacctg
    10441 gctaatatgg cggcagaatc ccgcgttggc ggtttgctcg gcagtaaacc tggcgtcacc
    10501 gtgttccaca tgggcgacag taaaaaggcg ttacagccta tctatgacct gctggaaaac
    10561 tgcgatgtgc cgatcagcaa gctgctgccg acccacgtta accgcaacgt accgttgttt
    10621 gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg atatcaccag cagcattgac
    10681 gaaccggtcg cccctgccga aggtattgcc cgcgccgttc aggcgggtat tccgctggca
    10741 cgcgtcaccc tcagctccga cggcaacggt agccagccgt tcttcgatga tgaagggaat
    10801 ttaacccata tcggcgttgc aggttttgaa acgttgctgg aaaccgtgca ggtgctggtt
    10861 aaagactatg atttcagtat cagcgatgcc ctgcgcccgc tcaccagtag cgtagccggt
    10921 ttccttaacc tgagcgggaa aggcgaaatt ctgccaggca atgacgcaga cttactggtc
    10981 atgacgccag aattgcgcat tgagcaggta tacgctcgcg gcaaactgat ggtcaaagac
    11041 ggcaaagcct gcgtgaaagg aacgtttgaa acggcttaaa cggttgtcag ccgccctaat
    11101 ggacgtagaa tgcccgcaag ggcggctgac agagtaaaac gtaatggatg actgtggtgc
    11161 ggttttgcat aatattgaaa ccaaatggct ttatgatttt ctgaccctgg aaaaatgccg
    11221 caatttttcc caagcggcag tcagtcgtaa cgtctcgcaa ccggcattca gccgccgcat
    11281 ccgcgcgctg gaacaggcga ttggcgttga attgtttaac cgccaggtga cgccgctgca
    11341 actctcagaa caagggaaaa tcttccattc gcagatccgc catctgttgc aacagttaga
    11401 aagtaacctt gcagagctgc gtggcggcag cgattacgcg caacgtaaaa tcaagatagc
    11461 cgctgcacac tctctttccc tcggtctgtt accgtccatt atcagccaga tgccgccgct
    11521 ctttacctgg gcaattgaag ccattgatgt cgatgaagca gtcgataaac tgcgtgaagg
    11581 gcaaagtgac tgtatttttt cctttcacga cgaagatttg ctggaagcgc cgtttgacca
    11641 cattcgctta tttgaatctc aattgttccc cgtttgcgcc agtgacgaac acggagaagc
    11701 actttttaac ctcgcgcagc cacactttcc gttactgaat tacagccgca actcctacat
    11761 ggggcgattg attaatcgca ccctgacgcg ccacagtgag ttaagtttca gcaccttttt
    11821 tgtctcttcg atgagcgagc ttttaaagca ggttgccctc gacggctgtg ggattgcctg
    11881 gctgccggaa tacgccatcc aacaagaaat tcgcagcggg cagctcgttg tgctgaatcg
    11941 ggacgaactg gtgatcccga ttcaggccta cgcataccgg atgaataccc gcatgaatcc
    12001 cgttgccgaa cgcttctggc gtgaactgcg cgagctggag attgtgctta gctgacattc
    12061 tcaagcgtcg cactgcgcca accgccatta tcccagacca gatcaaacgt tagcggttca
    12121 tcgtcgtggt acagcattat ctcaaggctg atcaccagcg tgtggcagta cgcttctttc
    12181 aaattaacct gcaacgccgc aatccgtggc tcatgaaata acagtgcaga acgcagctga
    12241 cggcaatacc agctctggag atgtttgtcg ctcgggttat gcacttgcca ctcgggcatg
    12301 cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat taaaaagagt gaggagatcg
    12361 ctgcataccg atttacgcag caatgacgtt ttattgcctg aaatttcagg taaaacagcc
    12421 tgaagtgatt ggcgcatcat tttgcgcact cctgacgttt agcaacatat gcggcttgcc
    12481 ctgaacaccg ggcaccactg tattgatgct cattttccat agccattccc ttttgttcat
    12541 tacatccatg tcagtctgag acaggcctga atcctgtcag caaagtataa aaccttactt
    12601 gcctatgaat atctactgac ttttcaggaa aaaagtcatg atttgataaa aatcatcaaa
    12661 ctcaaaaaaa aaccgctagt atattctgaa tattccgaat agttacgtag atgaaaatac
    12721 ttgtcaccgc cgctacttaa ttccggtggg aaactattta tttccgcata atcagccatg
    12781 tattatttga aacaattagt ttgtaaataa tataaattga taacatatca aatacatcaa
    12841 aaataccatt cataggcatt attaatatag acatcaatat agcaacatca aattccaatt
    12901 agtaataaga tatgcttttt aattgcatga aaataatttc aaatgagcgt tttttgatac
    12961 tttagagtgt ttcctaccct ttaatatcac cgtgtagagt tgat
//
LOCUS       DAZGBC010000143        12916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGBC010000143 DAZGBC010000000
VERSION     DAZGBC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.144"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..885
                     /gene="yhfZ"
                     /locus_tag="ACHK1J_003905"
     CDS             <1..885
                     /gene="yhfZ"
                     /locus_tag="ACHK1J_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO7913113.1"
                     /translation="KEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQAALK
                     TLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEGLASGLKA
                     QFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALELGPHTYV
                     GEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESLQRIVKGD
                     VDAVIWNVVAENELTTLGLEATPLTDDPRFLQATEAVVLTRVDDYPIQQLLRAVVDKH
                     ALLAHQQRVVSGEQEPSY"
     gene            902..1264
                     /gene="yhfY"
                     /locus_tag="ACHK1J_003906"
     CDS             902..1264
                     /gene="yhfY"
                     /locus_tag="ACHK1J_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO7913114.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSTHWPAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            1348..2511
                     /gene="yhfX"
                     /locus_tag="ACHK1J_003907"
     CDS             1348..2511
                     /gene="yhfX"
                     /locus_tag="ACHK1J_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO7913115.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNPLEA"
     gene            2512..3738
                     /gene="yhfW"
                     /locus_tag="ACHK1J_003908"
     CDS             2512..3738
                     /gene="yhfW"
                     /locus_tag="ACHK1J_003908"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7913116.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFTTHQTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLALLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVTTQIGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            3735..4613
                     /gene="php"
                     /locus_tag="ACHK1J_003909"
     CDS             3735..4613
                     /gene="php"
                     /locus_tag="ACHK1J_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO7913117.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYSFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            4624..4977
                     /gene="yhfU"
                     /locus_tag="ACHK1J_003910"
     CDS             4624..4977
                     /gene="yhfU"
                     /locus_tag="ACHK1J_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO7913118.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            4989..6293
                     /gene="yhfT"
                     /locus_tag="ACHK1J_003911"
     CDS             4989..6293
                     /gene="yhfT"
                     /locus_tag="ACHK1J_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO7913119.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDACGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFIPA"
     gene            6305..7390
                     /gene="yhfS"
                     /locus_tag="ACHK1J_003912"
     CDS             6305..7390
                     /gene="yhfS"
                     /locus_tag="ACHK1J_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7913120.1"
                     /translation="MKTFPLQSLTLNEAQQKQFALVDTICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RLAGIPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAHALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(7550..8281)
                     /gene="frlR"
                     /locus_tag="ACHK1J_003913"
                     /gene_synonym="yhfR"
     CDS             complement(7550..8281)
                     /gene="frlR"
                     /locus_tag="ACHK1J_003913"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO7913121.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNQRH"
     gene            complement(8381..9166)
                     /gene="frlD"
                     /locus_tag="ACHK1J_003914"
     CDS             complement(8381..9166)
                     /gene="frlD"
                     /locus_tag="ACHK1J_003914"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO7913122.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPELVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(9166..9993)
                     /gene="frlC"
                     /locus_tag="ACHK1J_003915"
     CDS             complement(9166..9993)
                     /gene="frlC"
                     /locus_tag="ACHK1J_003915"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO7913123.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            complement(10043..11065)
                     /gene="frlB"
                     /locus_tag="ACHK1J_003916"
     CDS             complement(10043..11065)
                     /gene="frlB"
                     /locus_tag="ACHK1J_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO7913124.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGEQASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(11086..12423)
                     /gene="frlA"
                     /locus_tag="ACHK1J_003917"
     CDS             complement(11086..12423)
                     /gene="frlA"
                     /locus_tag="ACHK1J_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO7913125.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAVTAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(12719..12886)
                     /gene="yhfL"
                     /locus_tag="ACHK1J_003918"
     CDS             complement(12719..12886)
                     /gene="yhfL"
                     /locus_tag="ACHK1J_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO7913126.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         2948 a         3250 c         3662 g         3056 t
ORIGIN      
        1 aaagaaggcg tcgttatcac gacgctggcc cgttatttgt tgggtgaaaa gtgcggtaat
       61 cgactgaaaa ctatagatga actggccact gaatgccgtt catccgttgg tctgacgcag
      121 gccgcgttga aaacgctgga atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat
      181 ggtagttatc tggtcgagat ggataacaaa gcattgctga gtcatgtgga tatcaacaac
      241 gtggtatgtg cgatgccttt gccctatacc cgcttgtacg aaggtttagc gagcggattg
      301 aaagcccagt ttgatggcat tcctttttac tatgcgcata tgcgcggcgc ggatattcgc
      361 gtggagtgtt tacttaacgg cgtgtatgac atggcggtgg tttcccgact ggcggcggaa
      421 agttatctca cgcaaaaagg gttatgcctc gcgctggagt tggggccgca cacctacgtt
      481 ggcgagcacc agttgatttg ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg
      541 gataaccgtt cggcggatca gaaaatcatg actgatgtct tttttggcgg tagtgatgtg
      601 gaacgagtcg atctctctta tcacgagagt ttacaacgca ttgttaaagg cgatgttgat
      661 gcggttatct ggaacgtggt ggcggaaaac gaactgacca cgctgggatt agaggcgacg
      721 ccgctcacag acgatccgcg atttttacag gccaccgaag cagtggtcct gacgcgagtc
      781 gatgattacc caatacaaca actgctgcgt gcagttgttg ataaacacgc cctgcttgcc
      841 catcaacaac gggtggtgag tggggaacag gaacccagtt attaaacgaa aggcatctga
      901 tatggaaacc agactcaacc tgctttgcga tgcaggcgtt attgataagg acatctgcaa
      961 aggcatgatg caggtcgtca acgtactgga aacagagtgc catctaccgg tgcgcagtga
     1021 gcaaggaacg atggcgatga cacatatggc gagtgcactg atgcgcagtc gccgtggtga
     1081 agaaatagag ccgctggatg acgaattgct ggcagagctg gcgcaatcca ctcactggcc
     1141 tgccgttgtg caattgcatc aggtgttgtt gaaggaattc gcactggaag ttaacccgtg
     1201 tgaagaaggc tatttgctgg ccaaccttta tggattatgg atggctgcta acgaagaggt
     1261 ttgaagctcc cgacaaatcc acgtcgtaca atctaaatat tcaaaaaaat gaatatttaa
     1321 ttcaaggaac agaaacgagg caacaagatg tttgtagaag cattgaaacg ccagaacccg
     1381 gcgctgattt ccgccgcact aagtttgtgg cagcagggca agatcgcccc ggacagctgg
     1441 gtgatcgacg tggatcaggt actggaaaac ggtaaacggc tgattgagac ggcgcggctt
     1501 tacggcattg aactgtatct gatgaccaaa caattcggtc gtaacccatg gctggcggaa
     1561 aaactgctgg cgttgggcta cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc
     1621 atgcgccgcg ctggtttgcc ggtggcgcat caggggcatc tggtacaaat cccttgtcat
     1681 caggttgctg acgccgttga acagggcacc gacgtcatca ccgtgtttac tctcgacaaa
     1741 gcgcgggaag tttctgcggc ggcggtgaag gccgggcgaa tccagtctgt gctgctcaaa
     1801 gtttatagcg acgatgattt tctttatccg gggcaggaga gcggttttgt acagcattcg
     1861 ctgcacgagg tggttgctga aattaagaaa ctgccggggc tgcatttagc cggactaacc
     1921 catttccctt gcctgctttg ggatgaggct gccgggaaag ttttgccgac accgaatctt
     1981 cacacgctga tacaggcacg ggatcaactg gcgaaatctg gtattgcgat tgagcaactg
     2041 aacgcgcctt cagcgaccag ctgcacttcg ctgccattgc tggcggaata cggtgcgact
     2101 catgccgaac ccggtcatgc gctgaccggt actattccgg caaaccagca gggcgatcag
     2161 cctgaacgta tcgcgatgct ctggttaagt gaaatctccc atcatttccg tggcgacagc
     2221 tactgctacg gcggcggtta ctaccgtcgt ggtcatgcgc aacatgcgct ggtgtttacg
     2281 ccagaaaatc aaaagattac tgaaaccaat ctcaaaactg tggatgacag cagtatcgac
     2341 tacaccctgt cgctggcagg cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc
     2401 acgcagattt ttgtcacccg tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa
     2461 ccgaaaatcg ttggtcgtta tgacagtctc ggcaaccctc tggaggcgta aatggcgcga
     2521 tttgtggtgt tagtgattga tagctttggc gtaggggcaa tgaaagatgt cacgctggtg
     2581 cgtccgcagg atgccggggc gaatacctgt ggtcacatcc tgagccagtt gccgcatttg
     2641 cagctaccaa cgctggagaa gctggggcta atcaacgcat tgggttatgc gccaggcgat
     2701 atgcagccgt cagactccgc aacctggggc gtggcagagc tgcaacatga aggtggcgat
     2761 acctttatgg ggcatcagga aattttaggc acgcgcccgt taccgccgct gcggatgccc
     2821 tttcgcgatg tgattgaccg tgttgagcag gcattagtat ccgccggttg gcaggtggag
     2881 cgccgtggcg atgaactgca atttctgtgg gtcaatcagg cggttgcgat tggcgataat
     2941 ctcgaggcgg atttaggcca agtctataac attaccgcca atctctctgt gatctctttt
     3001 gacgacgcaa tcaaaattgg tcgtatcgtg cgtgaccagg tacaggtcgg tcgggtcatt
     3061 acatttggcg gcctgttaac cgacagtcag cgtattctcg atgccgcaga aagcaaagaa
     3121 gggcgcttta ttggtattaa tgcgccgcgt tctggcgctt atgacaacgg tttccaggtc
     3181 gtgcatatgg gctatggcgt cgatgaaaaa gtgcaggtgc cacaaaaact gtatgaagca
     3241 ggcgtgccaa ccgtgctggt gggtaaggtg gcagatatcg tcaacaatcc ttatggcgtg
     3301 agctggcaaa atctggtgga tagccagcgg attatggata tcactctcga cgaatttacc
     3361 acccatcaga cagcgtttat ttgcaccaac attcaggaaa ccgacctcgc tggtcacgcg
     3421 gaagacgtcg cacgttatgc cgaacgtttg caggttgttg accgtaatct tgctctcctg
     3481 gttgaggcga tgcagccaga tgattgcctg gttgtcatgg ctgatcacgg taacgatccg
     3541 accattggtc acagccacca tacccgtgaa gtggtgccag tgctggttta tcagcaaggg
     3601 ctggtcacta cgcagatcgg tgtgcgcacc acgctttctg atgtgggggc taccgtgtgt
     3661 gaatttttcc gtgcgccacc gccacaaaat ggtcgctctt ttctttcctc cctccggttt
     3721 gcaggagaca ccctatgagt tttgatccga cgggttacac cctcgcccat gagcatctgc
     3781 atattgatct ctccggcttt aaaaacaacg tggactgccg ccttgatcag tattcgttca
     3841 tttgccagga gatgaacgac ctgatggccc ggggcgtgcg taatgtgatt gagatgacca
     3901 accgttacat ggggcgcaat gcgcaattta tgctcgatgt aatgcgcgag acggggatca
     3961 acgtggtggc ctgtaccggt tattaccagg acgcgttttt cccggaacat gtggcgaccc
     4021 gcagcgtgca ggaactggcg caggagatgg tcgatgaaat tgagcagggt atcgatggca
     4081 cggaactgaa agccgggatc atcgcggaga tcggctccag cgaagggaag attacgccgc
     4141 tggaagagaa ggtatttatt gccgccgcgc tggcacataa ccagaccgga cgcccgatct
     4201 ccacgcatac gtcgttcagc acgatggggc tggagcaact ggctttgttg caagcccacg
     4261 gagttgatct ttcgcgcgtc accgttggtc actgcgatct gaaagacaac ctcgacaaca
     4321 ttttgaagat gatcgatctc ggcgcgtatg tgcagttcga caccattggt aagaacagtt
     4381 actacccgga cgaaaagcgt attgcgatgc ttcatgcgct acgcgaccgt gggttgctga
     4441 accgcgtcat gctgtcgatg gatattacgc gccgctccca tttaaaagcc aacggtggtt
     4501 atggctatga ctatttacta accaccttta ttccgcaatt gcgccagtca ggattcagtc
     4561 aggccgatgt ggatgtgatg ttacgtgaaa atccctctca atttttccaa taaggacaga
     4621 ctcatgaaaa agattggcgt tgcaggctta cagcgtgagc agattaaaaa aactattgaa
     4681 gcgacggctc ctggctgttt tgaagttttc attcacaacg acatggaagc ggcaatgaag
     4741 gtgaaatccg ggcaactgga ttattacatc ggcgcgtgta ataccggtgc gggcgcggca
     4801 ttgtcgattg ccatcgcggt gattggctat aacaaaagtt gcaccattgc caaaccaggc
     4861 attaaagcga aagacgagca tatcgccaaa atgatcgctg aaggaaaagt ggcgtttggc
     4921 ctttccgttg agcacgtcga acacgcgatt ccgatgctga ttaaccatct gaaataaaag
     4981 gcacgactat ggatctgtat attcagatta tcgtggtggc gtgcctgacg ggtatgacat
     5041 cgcttctggc gcatcgctcg gcggctgttt ttcatgacgg catccgcccg atcctgccgc
     5101 aactgattga aggctatatg aaccgtcgcg aggcggggag tatcgctttt ggtctgagca
     5161 ttggttttgt ggcctcggtg gggatctctt ttaccctgaa aaccgggctg ctaaacgcat
     5221 ggttactctt tcttcctacc gatatcctcg gcgtactggc gataaacagc ctgatggcgt
     5281 ttggtcttgg cgctatctgg ggcgtgttga tccttacttg cctgttgcca gtaaaccagc
     5341 tgctgaccgc gctgccggtg gatgtattag gtagcctcgg ggaattaagc tcgccggtgg
     5401 tttctgcttt tgcactgttc ccgctggtgg cgattttcta ccagtttggc tggaagcaaa
     5461 gtctggtcgc cgccgtggtt gttctgatga cccgtgtggt agtcgtgcgc tatttcccac
     5521 atcttaaccc tgaatccatc gaaatcttta ttggcatggt gatgctgctg gggatcgcga
     5581 taactcacga cctgcgtcat cgtgatgaaa atgacatcga tgcctgcggg ctttcggtgt
     5641 ttgaagagcg cacgtcgcgg attatcaaaa acttaccgta tatcgccatc gtaggggcgc
     5701 tgattgccgc cgttgccagc atgaagattt tcgccggcag tgaagtgtcg atcttcactc
     5761 tggagaaagc gtactccgca ggcgtaacgc cggaacaatc acaaacgctg attaatcagg
     5821 cggcactggc ggaatttatg cgcgggctgg gttttgtgcc gttgattgcc accaccgcgt
     5881 tagccactgg cgtgtatgcg gttgcgggct ttacctttgt ttatgcggtg ggctatctct
     5941 caccgaatcc gatggttgcg gcagtattag gcgcagtggt tatttcggcg gaagtcttgc
     6001 tgcttcgttc tatcggcaaa tggctgggac gctacccgtc ggtgcgtaat gcgtcggata
     6061 acatccgtaa cgccatgaat atgctgatgg aagtggcgct gctggtcggt tcgattttcg
     6121 cagcaattaa gatggcgggt tataccggat tctctatcgc ggttgccatt tacttcctca
     6181 acgaatccct gggccgtccg gtacagaaaa tggcggcacc ggtcgtggca gtaatgatca
     6241 ccggcatcct gctgaatgtt ctttactggt ttggcctgtt tattccggct taaggaatgc
     6301 acctatgaag acgtttcctc tgcaaagcct gacgcttaat gaagcgcagc aaaagcagtt
     6361 tgcgttggtg gacaccatct gccgccattt ccccggcagc gagtttctta ctggcggtga
     6421 tttagggtta actccagggt tgaatcaacc gcgcattacc cagcgggtgg agcaggtgct
     6481 ggctgatgcg tttcatgcac cagcggcggc gctggtacag ggggcgggta ctggcgcgat
     6541 tcgtgcggcg ctggcggctt tgctcaaacc ggggcagcgt cttctggtgc atgacgcgcc
     6601 tgtttaccca acgacacggg ttattattga gcagatgggg ctgacgctta ttactgctga
     6661 tttcaatgac ctgtcggcac tgaagcaggt cgtcgacgag caacaacctg atgccgcgct
     6721 ggtgcagcat acgcgccagc agccgcagga cagctatgtg ctggcagatg tgctggcaac
     6781 gttgcgcctg gctggcattc cagcgttaac cgatgacaac tatgcggtga tgaaggtggc
     6841 gcgaatcggc tgtgaatgcg gcgcgaatgt ctcgacattc tcctgcttca agctgtttgg
     6901 gccggagggc gttggtgcag tggttggcga tgccgatgtt attagtcgga ttcgcgccac
     6961 gctttactcc ggcggcagcc agatccaggg cgctcatgcg ttggaagtgt tgcgtgggct
     7021 ggtgttcgcg ccagtgatgc acgcggtgca ggcaggggta tctgaacggt tgctggcttt
     7081 gctcaacggt ggtgcagtgc cggaagtgaa aagcgccgtc attgccaatg cgcagtcgaa
     7141 ggtgttgatt gtcgagtttc atcagccgat tgccgccagg gtgctggaag aagcgcaaaa
     7201 gcgtggcgcg ttgccttacc cggtcggtgc agagtcgaaa tatgaaatcc cgccgctctt
     7261 ttatcgcctt tccggaacgt tccgccaggc gaatccgcaa ctcgaacatt gtgcgattcg
     7321 cattaacccg aatcgcagcg gtgaagagac ggtgctgcgg attttgcgtg agagtattgc
     7381 cagtatttaa tgctggatag caccgttcgg caatcaagct aaattccgca ctgaacggtc
     7441 ccctcgcccc tttggggaga gggttagggt gaggggaacg tgcgggatgt aaaattcagt
     7501 gcgccggcat cgccggatgc gatgctggcg catcttatcc ggcctacggt taatgtcgtt
     7561 ggttatcaat cgttaaggtg atgcggttgg cgcggcagaa gagttcggag acgtgcaccg
     7621 gtttgtcatt ctgatcaaag gcgattttgc tgatgcgaaa caacggttcg cccagttcgc
     7681 atttcagcca ttttgcctgc gggcgggtgg cggcaaagat atcgatggtc tttttgtcgc
     7741 tgaccactcg cgtgtcaaaa cgctcctgaa ataactgata ggtggagctt ccttcgacgt
     7801 aaatctcatc aaagtcagga taacgcgaca gcgggatcca ggaactatca ataaacagcg
     7861 gctctttatc gagatacatc acccggcaaa gatggaacac ttcgctgttg ccagggatgt
     7921 tcagcttttc gcaaaacggc gcggcgctga cccgttcctg ctcgatcact ttctctttcg
     7981 tcgccttgcc ttgtgagacg ccaaaatcgg taaaaccact gacagtaagc agggcgtttt
     8041 cgaccttctg gctttgtaca aaggtgcctt tgccctgcca gcggatcagt acgccgtctg
     8101 ccactaagtc gctgatggct ttgcgaatgg taatgcggct gacgttatat tgtgtacaaa
     8161 gctcgttttc ggtagggatc tgttgcccgg cctggtaaac cccctgcgcg atatcatcca
     8221 gcagccgctg gcggacggta gcgtagagga gttgatgaga gtagcggtcc gtagctgaca
     8281 taatcgaccc tgaaataaag aatgaattac tcggattata gggtcgtttt ccggcaagta
     8341 acagcacgtt ggcgtgaaga tgctcacgcc aacgttatac ctaccaggca ccgtggtact
     8401 gaatggtttt cgccgcgcac gccgttccct gcgccatcgc ctgcggtaat gtcatccccg
     8461 cagaccagcc gcaaaggaat ccggcaatga acgaatctcc ggcacccatg gtgtcgataa
     8521 ccgtcaccag ttcaggagcc tgacgccaga actgcgcgcc atcccaggca atgctgccgt
     8581 tttcgcccag cgtgacaatc actgttcctg cgccacgggc aacaatcgct ttcatcttca
     8641 gacgcagcgc ttcgtcttct tgcggtgcag aggcaaaggc aaaatcgaga tgcggcacca
     8701 gtgtctgcca gagcgggctg tcccacttgt cggagaagtc gaaagcggta agtttgcccg
     8761 cagcgtgcag ctgtgggaat gcgtcttccg catgtcccca gattgccgcg tgcacaatgt
     8821 catactgcgc cagccaggcg taatcttctt cgctcagggc aaagtcggcc atcacgcctt
     8881 cggtgtagtc gccaaaaacg cgatcgttgt cgtgcagttc cacctgagtt tgtgcggtta
     8941 cgccgtgttt cgtatggaca tggctgatat cgacgcccat gcgggcgaga tcctgcttca
     9001 gctttgtgcc gtagtcatcg tcacccaccc aggtaatgca tcccggcttt ataccgtagc
     9061 gagtgcagta caccgccacg ttcaccgcat taccgccaga aaacgcttta ttcagttgcg
     9121 ggtagatatc gacgcagtta tcgccgattg tcgccagggt tttcatcact catcctccgg
     9181 cagcagcgcg cgaaagcgtt ccagcgcctg gcgggcgtaa agtctgggct cgttcatata
     9241 catcgttacc agttccactg tgcagtaccc ctcatagccc cgatcaataa tatcgcgcat
     9301 cagttcccgc agcggcattt tgccttctcc aggaatgtaa tgcgtgtcgc tggccccgtc
     9361 gctgtcgaca atatgcagat gacgtaattt atcgcccagt ttgtcgaaat aactcatcac
     9421 cggttccgcc tggacatacg gcgcgcaaat gtcgaccatg ctgaacaggc gcggcgaagg
     9481 caccagcgcc agcgcatgaa gcacatcatt agcattacac acgacgttcg attcatacgg
     9541 cgttaacggt tcgaggatca gatccatgcc gatgttttcc gcgtactcac acagttcgct
     9601 taagttctct gccagccgcc cccagataac gttaggcggc gtgagatagc ccgcgtgggc
     9661 cgcagaaatc agcgtataac ccgcgttcat ctcttttgcc atatccatcg ccagcttgat
     9721 catgtccagg ctttcgcgac gcatatgttc atcgcccagc atcatgttat acggatagcc
     9781 gttggtttct ggcgtataac cgataatcgg catctgatac gtctgcgcca gcgccttgat
     9841 ctgtttgatg ccgcccgctt ttaagtccgg cgcgaacgcg tgcgggcgac cgccccaaat
     9901 ttcgatgccg tcgtaaccca gctcgcttgc atcacggaat gcgtgttcaa taggcagccg
     9961 ctggtggccg caggtaaaca tacctgtttt catcgggtaa ctccttgcgt aaatccggcc
    10021 cgttgccggg ccgggagagg gattaatatt ccaccagacc accgtaatag cggcgttcat
    10081 ccgggttgtg atctttgtaa atagacaggt agtagcagag ccactccatt ggcacgaaca
    10141 tcaggaacgg tgccagccac gggtgcagcc cttgcgaaat ttcggcgtaa tcgatgacga
    10201 tcacgttgtc agtacgctgt ttaacaaagt taatggcgcg ttcggtggtg tgacgacttt
    10261 cgtcattgcc gagcagaaac aggaacggaa cgcccggttc gacaatctcc agcgggccgt
    10321 gacggaactc accgctctca atcacacagc cgtgcgtcca ggtaaattcc atcagcgtca
    10381 cgatgccttc tttgtaaccc agcgggcgca gcgggcctgc ggcaaccgtg taaatcatcg
    10441 gccactgact ggcctgttca ccgagctggc gacctttctc ttcccaggtg cgtaccagat
    10501 gaccgagcgc atttggcagc tgcttgagat cgtttttgat cttgccgatt tccgcgtgcg
    10561 gcgcgaggcg ggtgatcatc tccagcacca cgctgtagca gagcagcaga tgaatttccc
    10621 agatacagtc ggcctgataa tcaatgctaa attccgccgc cgaggtaatc gggctgtccg
    10681 cgcgtttggt gaacgccgca gtgagtgcgc cgcaggcccg gcccagctcc agcgctttga
    10741 ttacctcttc ggttttaccg tagtcagaaa cgccaattac tgcgcaacga tcgtcgaggc
    10801 gatacggggt gttatcgcag aactcccagc cggaaatggc gtaaacctga agatcggaaa
    10861 agcgatctgc cagatgtttc gccgtttgcg cggcgttgag tggcgatccg caggcaacga
    10921 aataaatacg atcgatgtcg cgcttcacca tctcttccac gatggtgtgc accagcggaa
    10981 cgtcatggct gagaactttt tccacttcct gaaccatatt ttcggtgacc agaaagtcca
    11041 cggtgctttt atcaatatcc aacattttat tttctccaga taacattacg acaaagcgtt
    11101 gagctggcgg ctacgcttcg cccagaaggc gtaagcaggc agaccagtgg caataacgat
    11161 gacggcgcag ataaggccgg gaatcggtgc ccagacaaag gttgaggcaa ccagaatgag
    11221 gcttgatgca atggcgaggg tggtcatcag cccgaaagcc ggagtacgcc acagcggttt
    11281 gtaatcgtcg cgtttacgac accagatgat ggagccgaag gtgagggtat ttttgaaaca
    11341 cattaccagg gtgaaatagc ccagcaggct ggtgagatct gaaacgaaga tgaagaagat
    11401 ccccagcgcc ccttgcagga tgatggagac atccggcgtg ttgtatttcg gatgcacatg
    11461 gccgaagcat ttgaagaaca gattgtcttt tgccatcgca tattccagcc gcggctggta
    11521 catcacgcaa ctggaaagcg agccgagaat gacgatcatc gccgtgacgg caacgaagat
    11581 cccggcggtg ctgccgagtg ccgggatcca ggtcagtgcg tcggaaatcg gcgtttcaga
    11641 gttggcgagt ttgtcgaagg gcatcagccc ggaaatcacc aacgccagca gggtgtagag
    11701 caccagaacc agcagacagg aaccaattag cgctcgtggc atagttttac cggggttttt
    11761 aatttcgccg gtcatataac agatagaggc catgccggtg tacgaccaac tggtggcgga
    11821 gatccccgcc agtagcgcca taaagctgcc cgttgcgcca atcgcagtgg tggtaggggc
    11881 ggcaaaattc tccgctttaa accagaagat cccaaggcca atgacgatag tgaacgggat
    11941 aattttggcg atggtaatta gcgtctgaaa tgctgcgccg ccttcaacgg agcgcaggtg
    12001 cagcagcata aaggcgataa ttaatccggc ggcgataaat ttaccgagca acggatcgat
    12061 aggcgttaaa aagccaagat tgctgactat cgccagcgcc ataatcgaca atgacggcgc
    12121 atcgttggcc cagaagctgg cccagccgga gaggaaagcc agcggtcggc ttccggcatt
    12181 tttcagataa acataatctg cgccattttc cggataagcg gtggatagtt ccgcatagac
    12241 gcacatttgc gggatcacaa ttaacccgcc gatgacaaat gcgagcaccg tcagccacgg
    12301 cgtgcccgcc gcttttgcca cttcacccac agatacaaaa ataccggagc cgacggttgt
    12361 cccgacggcg attgcaagaa cggcccaaaa tccgagcttg cgttggagtt cctggcttcc
    12421 catagcgata cctttttctt gtccaacacg ttgtagggga gagtttgcag tcatttaaaa
    12481 ggtaacatga caatgcatga tgaatataac aacatatgat gttatgtaat gtggaggaag
    12541 atcacataat ttcgcacagg atcgcgctgt ggctgatgaa tgtggttatc caattgaaat
    12601 taaagtgaaa atatttttac gggtggggag caagaaggat atagaaacaa atacgccctc
    12661 ggaaaatcca gagggcgtcg ggcaattaaa ccggtgttag ccgatttctg tcagagactt
    12721 actgtgcagt aggaccgcaa ccgccaatga ttttagaaat agaaattgcc gggtgcagca
    12781 ggtagtcgta agagcagttc ttatcacggt tttcaatatg accagtacat gcagttaacg
    12841 tagccagtac cgcacctacc agtgcaactt taataaattt gttcatttac attccctatg
    12901 tattatatat tgatta
//
LOCUS       DAZGBC010000144        12898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGBC010000144 DAZGBC010000000
VERSION     DAZGBC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.145"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..360
                     /locus_tag="ACHK1J_003919"
     CDS             <1..360
                     /locus_tag="ACHK1J_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO7913127.1"
                     /translation="TRLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMF
                     NTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDN
                     INAVAMKITADIMQDAD"
     gene            complement(514..2163)
                     /locus_tag="ACHK1J_003920"
     CDS             complement(514..2163)
                     /locus_tag="ACHK1J_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO7913128.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2314..3663)
                     /gene="ghxP"
                     /locus_tag="ACHK1J_003921"
                     /gene_synonym="yjcD"
     CDS             complement(2314..3663)
                     /gene="ghxP"
                     /locus_tag="ACHK1J_003921"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO7913129.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4209..4673)
                     /gene="soxR"
                     /locus_tag="ACHK1J_003922"
     CDS             complement(4209..4673)
                     /gene="soxR"
                     /locus_tag="ACHK1J_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO7913130.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSVRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4759..5082
                     /gene="soxS"
                     /locus_tag="ACHK1J_003923"
     CDS             4759..5082
                     /gene="soxS"
                     /locus_tag="ACHK1J_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO7913131.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5085..6671)
                     /gene="pdeC"
                     /locus_tag="ACHK1J_003924"
     CDS             complement(5085..6671)
                     /gene="pdeC"
                     /locus_tag="ACHK1J_003924"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO7913132.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCSPAHQKRMLNIIRGYLYINELIYAHDN
                     HFLCSSLIAPVNGYTIAPADYKRDPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7100..7381
                     /locus_tag="ACHK1J_003925"
     CDS             7100..7381
                     /locus_tag="ACHK1J_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO7913133.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7480..8016)
                     /gene="ssb1"
                     /locus_tag="ACHK1J_003926"
     CDS             complement(7480..8016)
                     /gene="ssb1"
                     /locus_tag="ACHK1J_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO7913134.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8271..11093
                     /gene="uvrA"
                     /locus_tag="ACHK1J_003927"
     CDS             8271..11093
                     /gene="uvrA"
                     /locus_tag="ACHK1J_003927"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO7913135.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11128..11484)
                     /locus_tag="ACHK1J_003928"
     CDS             complement(11128..11484)
                     /locus_tag="ACHK1J_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7913136.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11488..11904)
                     /locus_tag="ACHK1J_003929"
     CDS             complement(11488..11904)
                     /locus_tag="ACHK1J_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO7913137.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12015..12728)
                     /gene="aphA"
                     /locus_tag="ACHK1J_003930"
     CDS             complement(12015..12728)
                     /gene="aphA"
                     /locus_tag="ACHK1J_003930"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO7913138.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3317 a         3393 c         3189 g         2999 t
ORIGIN      
        1 acgcggttaa caaatacgtt cctcgaaatg gttatatgta ccggctctaa tatggctaat
       61 gttaatttta ataatgccaa tttaagcaat tgccatttca actgttctat tttaacaaaa
      121 gcctgtatgt ttaatacccg tctctatcgg gttaatttcg atgaggctag cgtccaggga
      181 atgggcattt ccattctccg tggggaggaa aatatcccca ttgatagtga taccctggta
      241 acactacaga aattctttga agaagattgt acctctcata ctggcatgtc acaaactgaa
      301 gataatatta atgcagtcgc tatgaagatt actgcagata ttatgcaaga tgcagattga
      361 ggttaagccg aagaacagcc aatgaattga ctttacaaga gagataactt atttgtttta
      421 gaatcatgaa tgttagcccg agctaacctg ttaaaggtgc aaaagattgc acctttttca
      481 gattacctcg ggcctggtta cgtcgctttc ggcttactga ttttcctcaa tcaacaacgc
      541 ttcaagcaaa tcgagatcgt gcagtaattt ttgcagcgtt tcgttactga tctcccgcgt
      601 ggcgcgtagg tggtacagtt cagcacgttc agaacgcaat gccgccagac ggaagcgacg
      661 ctcaaggttc tcttcctgca cggaactttc aacgtcgtta cgtccatcgg cgcgacgacg
      721 caggttacca atgacgcgag aactgacctc cgtaagcagc tggttatcga tattctcttc
      781 ggtatccgcc gccagacgct cctccatttt ctggatcgcc acaatcgcca cttctgccgt
      841 tgccgctcgc gcaatacgtt cttctttcaa ttgttgcgaa tggtccgcga cttcaatgtg
      901 ttgtagcaga ataggcaaca tcaccacgcc gacaaacagc gagaagagaa tgacaccagc
      961 cgccagaaac accagctcat agcgcgccgg gaagacgtta ccatccggca agagcagcgg
     1021 gatagagagc acaccggcca aagtgatcgc cccacgcacc ccggcgaaag acgcgatcag
     1081 gatttctcgt gtggtccacg aaccaaactc catcggcttc ttcttcagga agcggttgct
     1141 gaactttttc atcgtccaca gccagccgaa acggaccagc atcagcgccg catatatcag
     1201 aataatatcg gtaaacagca tccagatttc gacgttaggg tcgatttctg ctgccatcag
     1261 cgacgtctcc agaatacccg gcagctgcag acctaacagc aggaatacca tgccgttaaa
     1321 gacaaattcc agcatcgccc aggtgctgtt tgcacgcagg cgcattgcca gcggcgcacg
     1381 gcgcatcaca ccggagcggg tgatggtcat cccggcggca accgcagcga ggatgccgga
     1441 aacgccaata tgttccgcaa tcagataaga agcgaatggc agcaggaaca acaggacgat
     1501 ctgcgtcgcg ggttcatcac cgccccagcg gctgaggaat cgcagcgagc gaccgtacag
     1561 ccagctaacc acaaaaccgg cgagaatacc gccaatggct actttcataa attcgaccgt
     1621 cgcaccgccg acggtgaaga tcatcgtccc catcgccact gctacggcaa acttcaacga
     1681 caccaggccg gaagcgtcat tcatcaacgc ttcgccctgc aaaatgccca tgattttttt
     1741 cgggatgcgc ccttcgccaa caatcccgga gagcgccaca gcatcggtcg gagaaagtac
     1801 cgccgccagc gcaaaggcgg ggatcagcgg aatgcccggc accacccagt aaataaggaa
     1861 gccaatgccg accacggtga ccaccaccag cgccagtgcg aggccgaaaa tctctcgacc
     1921 atgttcgaga aattcacggg tcggcgtttt ccagccatca gcgaacagca acggcgggat
     1981 aaacaagact aaaaagagtt caggatcaaa ctccacatgc aaaccaaacg tcggccacgc
     2041 cagtagcgca ccgatggcga tttgcataag cggaagcggg atctgaaagg gcatgacacg
     2101 agtgaccacc ccggacagcg agaccacgag ggtcattatc agtatggtga agaagatttc
     2161 catgcgttcc ctgtttgcga tgttttttat gatctgagaa agacgttatg cctgaattct
     2221 cattttttca gaatatcgca aaagctcaca caatgtgcgg agaaaataaa aacgggcagc
     2281 cattgccacc cgttttcaga atccgatgaa agattagata gcccagccac ccgcatagaa
     2341 ggtcaccagc gccacggcga taacgaccgt accgatgttc aacttgcgcc attcgccgga
     2401 aaccagacga ccaatcacca gggtcgcgaa gccgatcatg atgcctgtta cgatgttaca
     2461 ggtcagcacg atgaataccg ccgtaaccag acccgccatc gcatcaacaa aatcagcaaa
     2521 gtcgattttc gccacgttgc tcagcattag caggccaacg tacatcagcg ccggagccgt
     2581 tgcatacccc ggaacgaggt aagaaagcgg agagaggaac agaatcagca ggaacagcac
     2641 gccaacggtg atagccgtca acccggtttt accgcccgcc gccgtacccg ccgcagactc
     2701 gatgtatacc gctgccggag ctgcacccac caggccagag aaaacgctgc tcatggagtc
     2761 agtggtcagt gctttcccgc cgtcgatgat ctgcccatct ttatccagca ggttcgcctg
     2821 gccggcgacg gcacggatag ttccggtggc atcaaatact gccgtcatca ccagcgccag
     2881 aacgcttggc aggactacag gattcagcgc gcccataatg tccaggctgc caatcagtga
     2941 attgccgttt tcatcgctca atgaaggcat ggcgaaaacg ccggagaaat ggacgttagg
     3001 atcgaagatc aaaccgacaa ttgagatacc gataatggtc agcagaatgc caccagggac
     3061 tttcagtttt tccaggccga tgatcaccgc cagacctacc agtgacataa tcaccgggaa
     3121 ggtcgcgaaa tcacccagcg caacgggcag accatcaagc gggtttttaa tcaccagacc
     3181 gacaccgtta gcggcaatga gcagcaggaa cagaccgata ccgatccccg tgccgtgcgc
     3241 cacaccgtgc ggcaagttgc gcaaaatcca gctacggata cccgtggcag aaattaccgt
     3301 aaacagcaca cccatcagga acacggcacc cagcgcgaca ggtacgctaa tatgttgccc
     3361 cagcaccagg ctgaatgcgg taaacgccgt cagggagatg gcgcaaccaa tcgccaacgg
     3421 cagattagcc cacagaccca tcacgataga accgagtccg gcaaccagac aggtcgcaac
     3481 gaaaactgcc gcaggcggga agcccgcttt acccaacatg cctggaacga cgataaccga
     3541 gtagaccatc gccagaaacg ttgttaaccc ggcaaccact tcctgacgga cagtgcttcc
     3601 acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg gtacgcgctg atggcgtaga
     3661 cataagagaa catcccctga gagtttaatt ttcgtcaaga tgcgtggcgg acaatcccct
     3721 gccagcgaaa cgttatcttc ctgctccagg tttgcgacaa tgtgctgcgt cgcaaaaaag
     3781 caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa aagcaaacga ttatccggcg
     3841 atcatagcgg ctttttcaac cgaagtttgc tgcttttttc tcttttattg tcttgacgat
     3901 gaaaaaccag gcaattgatg cgcaaacgtt atcgtcgatg cgcaagattg cagcgttatc
     3961 gagccgtata ccgttctgga ttgcgataaa aaatcgtctg agatgacaaa aatattgcgg
     4021 ctaaaaagta tacttttaca ctggcgaaga aacgccggtg acatacaatt aaagcatcaa
     4081 caccaaccgg aacctccacc acgtgctcga atgaggtgtg ttgacgtcgg gggaaaccct
     4141 cctgtgtacc agcgggatag agagaaagac aaagaccgga aaacaaacta aagcgccctt
     4201 gtggcgcttt agttttgttc atcttccagt aagcgtgcgc cggtaccttc ttctcctaag
     4261 cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa ggcagccaca accaatacat
     4321 ccatccagtt cgtcacgcag cgccactaag gtatgaatgc gccgatccaa ctcttctcgc
     4381 cattgggacg aaagctgttt ccactctttc gcacttaacg tatgcccttc gggcaacacg
     4441 ccaaacgctt caccaatggt cgccagcgga atgccaatac gctgagcaat tttgataatt
     4501 gcgacatatc gcaacacatc acgtttatat cggcgctgat tgccgctatt acggacactg
     4561 gtaatcaacc ctttgctttc atagaaatgc agcgccgata ccgccacacc gctgcgtttc
     4621 gccacttcgc cgggggttag cagcgcttta atgcggggta atttcttttc cataaatcgc
     4681 tttacctcaa gttaacttga ggaattatac tccccaacag atgaattaac gaactgaaca
     4741 ctgaaaagag gcagatttat gtcccatcag aaaattattc aggatcttat cgcatggatt
     4801 gacgagcata ttgaccagcc gcttaacatt gatgtcgtcg caaaaaaatc aggctattca
     4861 aagtggtact tacagcggat gttccgcacg gtgacgcatc aaacgcttgg cgattacatt
     4921 cgccaacgcc gcctgttact ggccgccgtt gagttgcgca ccaccgagcg tccgattttt
     4981 gatatcgcaa tggacctggg ttatgtctcg cagcagacct tctcccgcgt tttccgtcgg
     5041 cagtttgatc gcactcccag cgattatcgc caccgcctgt agttttattg cccgcgcgtt
     5101 aactcccgcg cgggtaattg ctccatccat tgcataaaca cctgcggcgg catcgccttc
     5161 gcaaaaaacc atccctggca atagcgcacg ccgcgtttgc gcagccagtt aacctgctcc
     5221 tccgtttcga cgccttcagc gatcgttttt aaccccaggc tgtgcgccag ctcgatgata
     5281 tgttccgcaa tcaaatgact ggttttatgg gtggtcagcg tctcaacaaa cgatttgtcg
     5341 attttcaaaa tatcgacatt caatgattta aggttatgca agttagagta gccaatacca
     5401 aaatcatcaa ttgccacttc gtaaccggcc tggcggaaag ccagaataat cggcgtcatt
     5461 ttgtcgacat caagaaatgc atgttcagtc acttcaaatt taatttgctg tggacgcacc
     5521 gcgtattgct ctgttttctg attgattcgc gctatcaacc gtgacgtatg aaaatcggag
     5581 gccgacagat taatagaaac atagcgatca gcatgtgttg caaggtacgc gcccagatcg
     5641 cggaagacat tatcaataac ataatcagtt acctgttcta tcatcccctc cttttctgcc
     5701 agcggaataa actctgccgg attcattact tgcccctgct caccaggcca acgtaacaac
     5761 gcttcagcgc cgatacattt ttctgttttg atatcgatta ttggctggta ataaagacaa
     5821 agttgatgtt tttctagggc gcgttgcaat ttgcgtttgg gagacaaata gttttgtcga
     5881 atacgtagcc agagtaatag cagaacaaga ctccccaaaa taccggcggg taacgcaaaa
     5941 ataagatgat tataaaaatg ggctataaga cgttgatatg aagtcgcaac aatggctgca
     6001 attgggcgtt ttgtcgaata aactgtcgca tataaatagc catttctttg tacggttaaa
     6061 tccttcaaat gaatcagcgg agaaaacgtt gctgccgagg cctcgttgct taacgagaaa
     6121 aaggttttcg tcaccgtatc atacacaccc cattgcaatg tcgggtcatc agacatcact
     6181 tcactccaga agagagggtt gataaccgcc acataatttc cccgctgcat ataggtcatt
     6241 ttatagccag agaaaaaagg cgtatcgcgg taataataga tagaaacgtt agggtcacgc
     6301 ttataatcgg ccggtgcaat cgtatagccg tttacaggcg ctatcagcga tgagcataaa
     6361 aaatggttat catgggcata gatcaattca ttaatataaa gatagccacg aataatattt
     6421 aacattcgct tttgatgggc tggagagcaa acttgcccct gatagcgttc tgctgcatcg
     6481 ctcactaaat ctgcctggcg aatgaccagc tcggatttat ccagtgccag ttgagcaaag
     6541 gttcggagct gattattcac ttctgatttt gcccaaagga aggcaatcca tagcgaaaga
     6601 atgatgggaa agagaactaa aaagataatg cccggcaacg ccagtaattg gtgtcgtgca
     6661 cgatgactca ttgctatcaa tttcctgcct gtttataccc ggccattgca aggtgaaacc
     6721 ctggctaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga
     6781 aattgatacc tttttaatcg tcacctggat gaaaataatt cattgatatt caaaattaat
     6841 tttctacagg aatacagagg cggcgggaag atataccctt aatgccactg aaaaaccaca
     6901 taatctgtac agcaataaaa gtcacgacct aatctggcta acaaacaatc aattgtgata
     6961 tagttcacaa aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
     7021 gatagccaga gtgtaccacg cctcccgtga acaacgccgc gctgtccagg gcatcggctc
     7081 ttttcagagg aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca
     7141 acttcttagt gccgtaatga tgtttctggc cagcacgctc aacatccgtt ttcgtcggtc
     7201 tgattatgtc gggcttgcag tgatcagcag cggtctgggc gtggtttccg cctgctggtt
     7261 cgcgatgggg ttgcttggca tcacaatggc ggatatcacc gccatctggc acaacatcga
     7321 gtcggtgatg atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacctg
     7381 atacgtagaa gcctccataa cggaggcttc ttttttatgg ctggcagatg ctttaatcat
     7441 ccaccttaaa acaatataac ctattgtttt aatgacaaat cagaacggaa tgtcatcatc
     7501 aaagtccatc ggcggctcgt tagacggcgc tgccggagcg gactgctgcg ggcgagactg
     7561 cgcgccgccg ctgaactgat tgccaccctg cggctgctga ggctgacccc aaccgccctg
     7621 cggctgacca ccaccgatat tgccacctgc aggagcgcca ccaccctgac gaccacccag
     7681 catctgcata gtgccgccaa cgttcaccac aacttctgtg gtgtagcgat cctgaccaga
     7741 ttgatcggtc catttacggg tacgcagctg accttcgata taaacctgag aacctttacg
     7801 cagatattcg ctggccactt ctgccagttt gccgaacagc acaacgcggt gccattcagt
     7861 ctgctctttc atctcgccgg tcgctttatc acgccaggat tcggaagtag ccagcgtaat
     7921 gttggcaact gcgccaccat ttggcatgta gcgtacttcc gggtcctgac ccagattacc
     7981 aacgagaata accttgttta cgcctctgct ggccatgttc gtgtctcctg aaaaaaaatc
     8041 gttctgaata agtgtaaacg tgcgattgta ccattaccaa tagcgctttt actatgttgt
     8101 gacctcggtt ccgggaaaca aacctggcca gacattgtta cacaacactc cgggtaatgc
     8161 attccaatac tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc
     8221 cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga
     8281 tcgaagttcg gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg
     8341 acaagctcat tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca
     8401 ccttatatgc cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt
     8461 ttctgtcact gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct
     8521 caattgagca gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa
     8581 tccacgacta tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg
     8641 acgtcccgct ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg
     8701 aaggcaagcg tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca
     8761 aaacgctgga gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct
     8821 gcgatctttc cgatccgccg aaactggaac tgcaaaagaa acataccatt gaagtggtgg
     8881 ttgatcgctt caaggtgcgt gacgatctta cccaacgtct tgccgagtcg tttgaaaccg
     8941 cgctggagct ttccggtggt accgcggtag tggcggatat ggacgacccg aaagcggaag
     9001 agctgctgtt ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgcgagctgg
     9061 agccgcgact gttttcgttt aacaacccgg caggtgcctg cccgacctgt gacggccttg
     9121 gtgtacaaca atatttcgat cctgaccgcg tgatccaaaa ccccgagcta tcgctggctg
     9181 gcggtgcgat ccgtggctgg gatcgccgca acttctatta tttccagatg ctgaaatcgc
     9241 tggcagatca ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc
     9301 ataaagtggt gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc
     9361 gtggcgatac ctccattcgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc
     9421 gttataaaga gacggagtcc agcgcggtac gcgaagaatt agccaagttt atcagtaatc
     9481 gtccgtgtgc cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtgtatgtcg
     9541 agaatacgcc gctgcccgcc atctccgaca tgagcattgg tcatgcgatg gaattcttca
     9601 acaatctcaa actcgcaggt cagcgggcga agattgcgga aaaaattctt aaagagatcg
     9661 gcgatcgttt gaaattcctc gttaacgtcg gcctgaatta cctgacactt tcccgctcgg
     9721 cagaaacgct ttctggcggt gaagcacagc gtattcgtct ggcgagccag attggtgcgg
     9781 gcctggttgg tgttatgtat gtactggacg agccgtctat cggcctgcac cagcgcgata
     9841 acgagcgcct gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg
     9901 tggagcacga cgaagacgcg attcgcgccg ctgaccatgt gatcgacatt ggcccgggcg
     9961 caggtgtaca cggcggtgaa gtggtcgcgg aaggtccgct ggaagcgatt atggcggtgc
    10021 cggagtcgtt gaccgggcag tacatgagcg gtaaacgcaa gattgaagta ccgaagaaac
    10081 gcgttccggc gaatccggaa aaagtgctga agctgacagg cgcacgcggt aataacctga
    10141 aagacgtgac gctgacgctg ccagtcggtc tgtttacctg catcacaggg gtttcaggtt
    10201 ccggtaaatc gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg
    10261 gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca gggactggag catttcgaca
    10321 aagtgatcga tatcgaccaa agcccaattg gtcgtactcc gcgttctaac ccggcgacct
    10381 ataccggcgt gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc
    10441 gcggctatac gccgggacgt ttcagcttta acgtccgtgg cggacgctgc gaggcctgtc
    10501 agggcgatgg cgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg
    10561 accagtgcaa aggtaaacgc tataaccgtg aaacgctgga gattaagtac aaaggcaaaa
    10621 ccatccacga agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc
    10681 cagctctggc gcgtaagctg caaacgttga tggacgttgg tctgacgtac attcgcctgg
    10741 ggcagtccgc aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc
    10801 tgtcaaaacg cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc
    10861 acttcgccga tattcagcaa ctgctcgacg tactgcataa actgcgcgat cagggcaaca
    10921 ccattgtggt gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc
    10981 tgggaccaga aggcggcagt ggtggcggcg agatcctcgt ctccggtacg ccagaaaccg
    11041 tcgcggagtg cgaagcatca cacacggcac gcttccttaa gccgatgctg taatcgttaa
    11101 ggccgctttc tgagcggcct tttcctttca gagttgcacc agcaatttac gtttttcttc
    11161 cggcagtaaa ttcaccgcct gctgataaga cgcatccacc agataataga tttgcgaatc
    11221 tggcagcgaa ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggcttgg
    11281 acgcacatcg ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga
    11341 aacagctggg cgattttcta cttctttcac catcgcaaac agtacatctt caactttgat
    11401 ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg cctggttttg ccatgcaata
    11461 ttgtagcaac tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg
    11521 cgatccgccg tggatgcgat gttcccccag ccaaatgcct tgccaggtgc cggtctgaat
    11581 acgcccttta tgcaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga
    11641 aggcatatcg tctgctccct catagtcatg ctcatagttt ccattatcgg gaacggtgcg
    11701 gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag
    11761 agaggcggag gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc
    11821 agccagctga ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag
    11881 cgtgagcgtc ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg
    11941 cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagtgtcac
    12001 gctctcccgt tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct
    12061 tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg
    12121 acatcgcgtg cagcggtaat atcgttatcg gaatcgccat aaaaaattcg gatattttta
    12181 tcctgcagcc attgcgattt tgtattttgc cctggtttat cgcccgcaaa gatcaccgga
    12241 ttcatgttgg tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt
    12301 ttcgtcgggc tacgaccagt cacaaagaag atcgcgtcac cacggcgtac atgcatatca
    12361 atcagctggc gagcgacctc ttttggaatg ctgaattcat cccagccgtt gttcattttt
    12421 tcccagaata cagggttttt cagataatct tcgctttctg gcgagaaggt ttttttgccg
    12481 cgccagaagc ccgggctgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt
    12541 ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aattggtgcc
    12601 tgttcagcaa gcctggcaac gttagtccca gggttaagcg gtgaaggaga tgaggccagg
    12661 gcaacagcgg aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc
    12721 ttgcgcatat ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg
    12781 caaaaaccat ctgaccataa cgccagctgc aggggaaagg aaggattatc tgcggttttt
    12841 gtgatgaaaa gcaggatgat tatgtttaac ccagtaaata cgttcctgtc tttttttg
//
LOCUS       DAZGBC010000145        12760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGBC010000145 DAZGBC010000000
VERSION     DAZGBC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.146"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1124
                     /gene="proV"
                     /locus_tag="ACHK1J_003931"
     CDS             <1..1124
                     /gene="proV"
                     /locus_tag="ACHK1J_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO7913139.1"
                     /translation="GLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGKSTMV
                     RLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLDNTAF
                     GMELAGINAEERREKAIDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINPDILL
                     MDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNGEVVQ
                     VGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSALKLL
                     QDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLSELLS
                     HVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1117..2181
                     /gene="proW"
                     /locus_tag="ACHK1J_003932"
     CDS             1117..2181
                     /gene="proW"
                     /locus_tag="ACHK1J_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO7913140.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2239..3231
                     /gene="proX"
                     /locus_tag="ACHK1J_003933"
     CDS             2239..3231
                     /gene="proX"
                     /locus_tag="ACHK1J_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO7913141.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3423..4607
                     /locus_tag="ACHK1J_003934"
     CDS             3423..4607
                     /locus_tag="ACHK1J_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7913142.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYNDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4731..5468
                     /gene="ygaZ"
                     /locus_tag="ACHK1J_003935"
     CDS             4731..5468
                     /gene="ygaZ"
                     /locus_tag="ACHK1J_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO7913143.1"
                     /translation="MESPTTQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5458..5793
                     /gene="ygaH"
                     /locus_tag="ACHK1J_003936"
     CDS             5458..5793
                     /gene="ygaH"
                     /locus_tag="ACHK1J_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO7913144.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5884..6414
                     /gene="emrR"
                     /locus_tag="ACHK1J_003937"
     CDS             5884..6414
                     /gene="emrR"
                     /locus_tag="ACHK1J_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO7913145.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6541..7713
                     /gene="emrA"
                     /locus_tag="ACHK1J_003938"
     CDS             6541..7713
                     /gene="emrA"
                     /locus_tag="ACHK1J_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO7913146.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQTFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7730..9268
                     /gene="emrB"
                     /locus_tag="ACHK1J_003939"
     CDS             7730..9268
                     /gene="emrB"
                     /locus_tag="ACHK1J_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO7913147.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9332..9847)
                     /gene="luxS"
                     /locus_tag="ACHK1J_003940"
     CDS             complement(9332..9847)
                     /gene="luxS"
                     /locus_tag="ACHK1J_003940"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO7913148.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(9997..11553)
                     /gene="gshA"
                     /locus_tag="ACHK1J_003941"
     CDS             complement(9997..11553)
                     /gene="gshA"
                     /locus_tag="ACHK1J_003941"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO7913149.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11626..12054)
                     /locus_tag="ACHK1J_003942"
     CDS             complement(11626..12054)
                     /locus_tag="ACHK1J_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO7913150.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVATLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12051..12617)
                     /gene="yqaB"
                     /locus_tag="ACHK1J_003943"
     CDS             complement(12051..12617)
                     /gene="yqaB"
                     /locus_tag="ACHK1J_003943"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO7913151.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         2937 a         3382 c         3291 g         3150 t
ORIGIN      
        1 aaggactttc aaaagaacaa attctggaaa aaactgggct atcgcttggc gtaaaagacg
       61 ccagtctggc cattgaagaa ggcgagatat ttgtcatcat gggattatcc ggttcgggta
      121 aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc cacccgcggg caagtactga
      181 ttgatggtgt ggatattgcc aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa
      241 agattgcgat ggtcttccag tcctttgcct taatgccgca tatgaccgtg ctggacaata
      301 ctgcgtttgg tatggaattg gccggaatta atgccgaaga acgccgggaa aaagccattg
      361 atgcactgcg tcaggtcggg ctggaaaatt atgcccacag ctacccggat gaactctctg
      421 gcgggatgcg tcaacgtgtg ggattagccc gcgcgttagc gattaatccg gatattttat
      481 taatggatga agccttctcg gcgctcgatc cattaattcg caccgagatg caggatgagc
      541 tggtaaaatt acaggcgaaa catcagcgca ccattgtctt tatttcccac gatctcgatg
      601 aagccatgcg tattggcgac cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg
      661 gcacaccgga tgaaattctc aataatccgg cgaatgatta tgtccgtacc ttcttccgtg
      721 gcgttgatat tagtcaggta ttcagtgcga aagatattgc ccgccggaca ccgaatggct
      781 taattcgtaa aacccctggc ttcggcccac gttcggcact gaaattattg caggatgaag
      841 atcgtgaata tggctacgtt atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg
      901 attcgcttaa aaccgcgtta acgcagcagc aaggtcttga tgcggcgctg attgatgcgc
      961 cgttagcagt cgatgcacaa acgcctctta gcgagttgct ctctcatgtc ggacaggcac
     1021 cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa
     1081 tgctgctgcg cgctttagat cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg
     1141 gataccacgc cagcggcgga cagtgccgcg caatccgcag acgcctgggg tacaccggcg
     1201 actgcaccga ctgacggcgg tggtgctgac tggctgacca gtacgcctgc gccaaacgtc
     1261 gagcatttta atattctcga tccgttccat aaaacgctga tcccgctcga cagttgggtc
     1321 actgaaggga tcgactgggt cgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt
     1381 ccggttgatt atatcctcaa cggtttccag caattgctgc tgggtatgcc cgcgccggtg
     1441 gcgattatcg ttttcgctct catcgcctgg cagatttccg gggtcggaat gggcgtggcg
     1501 acgctggttt cgctgattgc catcggcgca atcggtgcct ggtcgcaggc aatggtgact
     1561 ctggcgctgg tgttaaccgc cctgctgttc tgtatcgtca tcggtttgcc gttggggatc
     1621 tggctggcga gaagtccgcg agcggcgaaa attattcgtc cactgcttga tgccatgcag
     1681 accacgccag cgtttgttta tctggtgcca atcgtcatgc tatttggtat cggtaacgtg
     1741 ccgggcgtgg tggtgacgat catctttgct ctgccgccga ttatccgtct gaccattctg
     1801 gggattaacc aggttccggc ggatctgatt gaagcctcgc gctcattcgg tgccagcccg
     1861 cgccagatgc tgttcaaagt tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt
     1921 aaccagacgc tgatgctggc cctttctatg gtggtcatcg cctcgatgat tgccgtcggc
     1981 gggctgggtc agatggtact tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt
     2041 ggcggcgtcg ggattgtgat cctcgccatt atcctcgatc gtctgacgca ggccgttggg
     2101 cgcgactcac gcagtcgcgg caaccgtcgc tggtacacca ctggccctgt cggtctgctg
     2161 acccgcccat tcattaagta actctgcact tgcccggtga caccgggcat atcaccctgc
     2221 caaaaaaaag gaataacaat gcgacatagc gtactttttg cgacagcgtt tgccacgctt
     2281 atctctacac aaacttttgc tgccgatctg ccgggcaaag gcattactgt taatccagtt
     2341 caaagcacca tcaccgaaga aaccttccag acactgctgg tcagtcgtgc gctggagaaa
     2401 ttaggttata ccgtcaataa acccagcgaa gtggattaca acgttggcta cacctcgctt
     2461 gcttccggcg atgcaacctt caccgccgtg aactggacac cactgcatga caacatgtac
     2521 gaagctgccg gtggcgataa gaaattttat cgtgaagggg tatttgttaa cggcgcggca
     2581 cagggttacc tgatcgataa gaaaaccgcc gaccagtaca aaatcaccaa catcgcacaa
     2641 ctaaaagatc cgaagatcgc caaactgttc gataccaacg gcgacggtaa agcggatttg
     2701 actggctgta accctggctg gggctgcgaa ggtgcgatca accaccagct tgccgcgtat
     2761 gaactgacca ataccgtgac gcataatcag gggaactacg cggcgatgat ggctgacacc
     2821 atcagtcgtt acaaagaggg caaaccggtg ttttactaca cctggacgcc gtactgggtg
     2881 agtaatgagc tgaagccagg gaaagatgtg gtctggttgc aggtgccgtt ctctgcactg
     2941 ccgggcgata aaaatgccga taccaaactg ccgaatggcg cgaattacgg cttcccggtc
     3001 agcaccatgc atatcgttgc caataaagcc tgggccgaga aaaacccggc agcagcgaaa
     3061 ctgtttgcca ttatgcagtt gccagtggca gatattaacg cccagaacgc cattatgcat
     3121 gacggcaaag cctcagaagg cgatattcag ggccacgttg atggctggat caaagcccac
     3181 cagcaacagt tcgatggctg ggtgaatgag gcgttggcag cgcagaagta atttttattc
     3241 gggcggataa ggcgtttacg ccgcatccgc cgttctgtac acaatgcctg atgcgacgct
     3301 ggcgcgtctt atcatgccta caaaccacac cgcacatttc aacaatctat ctttcatccc
     3361 atattcatca acatccgcta ttattgattt ccagcttaat catcacctga tgaacaaaaa
     3421 taatgaccaa acctaatcat gagcttagcc cggcgctaat cgtgctgatg tctatcgcca
     3481 ccggtctggc ggtagccagt aactattacg cccagccatt gctcgacacc atcgcgcgta
     3541 acttttccct ttccgccagt tcggcaggct ttattgttac cgccgcgcag ttgggctatg
     3601 ccgcaggtct actgtttctt gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg
     3661 tctcgatgac cttactggcg gcatgcggca tgttgattac cgccagcagt cagtcgctgg
     3721 cgatgatgat cctcggtacg gcattaaccg gtttattctc agtcgtggca caaattctgg
     3781 ttccactggc agcgacgctg gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta
     3841 tgagcggcct gctgttgggg atattgctgg cacgaaccgt tgccgggtta ctggcaaacc
     3901 tcggcggctg gcgcaccgtc ttttgggttg cctcggtgtt aatggctctg atggcgctag
     3961 cattatggcg tggtctgcca caaatgaaat cagaaaccca cctcaattac ccacagttgt
     4021 tgggttccgt tttcagtatg tttatcagca ataagatcct gcgcacccgc gcgttgctgg
     4081 gctgcctgac ctttgccaac ttcagcattc tctggacctc aatggccttt ttgcttgccg
     4141 caccgccttt taattacaac gatggcgtca ttggtctgtt tggacttgcg ggggctgccg
     4201 gagcgttggg cgctcgtccg gcgggcggtt ttgccgacaa gggcaaatcg caccacacca
     4261 caactttcgg tctgctgctg ctgttacttt catggctggc aatctggttt ggtcacactt
     4321 ccgtactggc gttgattatc ggcattctgg tgctggacct caccgtacag ggcgtgcata
     4381 tcactaacca gacggtaatt tatcgaatac atcctgatgc gcgtaatcgc ctgaccgcag
     4441 gttacatgac cagctacttt attggcggtg ccgccggttc gctaatttca gcctcagcct
     4501 ggcaacatgg cggttgggct ggcgtttgtc tggctggcgc gacgattgcc ctggttaact
     4561 tactggtctg gtggcgaggt tttcatcgtc aggaagccgc aaattaagca atcagcaacc
     4621 gttttcgtgg ctttacactt ataagggtgt taagaagccc atcagtctga taaggttaag
     4681 atattcattc agtctattta taatattaac aatcgttaag cgtacactct atggaaagcc
     4741 ctactacaca gcctgctcct ggttcggcga ccttcatgga aggatgcaaa gacagtttac
     4801 cgattgttat tagttatatt ccggtggcct ttgcgttcgg tctgaatgcg acccgtctgg
     4861 gattctctcc tctcgaaagc gtttttttct cctgcatcat ttatgcaggc gcgagccagt
     4921 tcgtcattac cgcgatgctg gcagccggga gtagtttgtg ggttgctgca ctgaccgtca
     4981 tggcaatgga tgttcgccat gtgttgtatg gcccgtcact gcgtagccgt attattcagc
     5041 gtctgcaaaa atcgaaaacc gccctgtggg cgtttggcct gacggatgag gtttttgccg
     5101 ccgcaaccgc aaaactggta cgcaataatc gccgctggag cgagaactgg atgatcggca
     5161 ttgccttcag ttcatggtca tcgtgggtat ttggtacggt aataggggca ttctccggca
     5221 gcggcttgct gcaaggttat cccgccgttg aagcagcatt aggttttatg cttccggcac
     5281 tctttatgag tttcctgctc gcctctttcc agcgcaaaca atctctttgc gttaccgcag
     5341 cgttagttgg tgcccttgca ggcgtaacgc tattttctat tcccgtcgcc attctggcag
     5401 gcattgtctg tggctgcctc actgcgttaa tccaggcatt ctggcaagga gcgcccgatg
     5461 agctatgagg ttctgctgct tgggttactg gttggcgcgg cgaattattg cttccgctat
     5521 ttgccgctgc gcctgcgtgt gggtaatgcc cgcccaacca aacgtggcgc ggtaggtatt
     5581 ttgctcgaca ccattggcat cgcctcgata tgcgctctgc tggttgtctc taccgcacca
     5641 gaagtgatgc acgatacacg ccgtttcgtg cccacgctgg tcggcttcgc ggtactgggt
     5701 gccagtttct ataaaacacg cagcattatc atcccaacac tgcttagtgc gctggcctat
     5761 gggctcgcct ggaaagtgat ggcgattata taacccacaa gaatcatttt tctaaaacaa
     5821 tacatttact ttatttgtca ctgtcgttac tatatcggct gaaattaatg aggtcatacc
     5881 caaatggata gttcgtttac gcccattgaa caaatgctaa aatttcgcgc cagccgccac
     5941 gaagattttc cttatcagga gatccttctg actcgtcttt gcatgcacat gcaaagcaag
     6001 ctgctggaga accgcaataa aatgctgaag gctcagggga ttaacgagac gttgtttatg
     6061 gcgttgatta cgctggagtc tcaggaaaac cacagtattc agccttctga attaagttgt
     6121 gctcttggat catcccgtac caacgcgacg cgtattgccg atgaactgga aaaacgcggt
     6181 tggatcgaac gtcgtgaaag cgataacgat cgccgctgcc tgcatctgca attaacggaa
     6241 aaaggtcacg agtttttgcg cgaggtttta ccaccgcagc ataactgcct gcatcaactc
     6301 tggtccgcgc tcagcacaac agaaaaagat cagctcgagc aaatcacccg caaattgctc
     6361 tcccgtctcg accagatgga acaagacggt gtggttctcg aagcgatgag ctaacgcgtc
     6421 atctcgctca aaaatccaga tttataaaag aaaaaatgac cggccagcat cgcaacatgc
     6481 tggccttttt ggcaagcagg tcggctcagc cgatgagtta agaagatcgt ggagaacaat
     6541 atgagcgcaa atgcggagac tcaaaccccg cagcaaccgg taaagaagag cggcaaacgt
     6601 aagcgtctgc tcctccttct caccttgctc tttataatta ttgccgtagc gatagggatt
     6661 tattggtttt tagtactgcg tcatttcgaa gaaaccgatg acgcatacgt ggcagggaat
     6721 caaattcaaa ttatgtctca ggtgtctggc agcgtgacga aagtctgggc cgataacacc
     6781 gattttgtaa aagaaggcga cgtgctggtc actctcgacc cgacagatgc tcgccagacg
     6841 tttgaaaaag ccaaaactgc actggcttcc agcgttcgcc aaacccacca gctgatgatt
     6901 aacagcaaac agttgcaggc gaatattgag gtgcaaaaaa tcgccctcgc gaaagcacaa
     6961 agcgactaca accgccgtgt gccgctgggc aatgccaacc tgattggtcg cgaagagctg
     7021 caacacgccc gcgacgccgt taccagtgcc caggcgcaac tggacgtcgc gattcaacaa
     7081 tacaatgcca atcaggcgat gattctgggg actaaactgg aagatcagcc tgccgtgcaa
     7141 caggctgcca ccgaagtacg taacgcctgg ctggcgctgg agcgtactcg tattgtcagt
     7201 ccgatgaccg gttatgtctc ccgccgcgcg gtacagcctg gggcgcaaat tagcccaacg
     7261 acgccgctga tggcggtcgt tccagccacc aatatgtggg tggatgccaa ctttaaagag
     7321 acgcagattg ccaatatgcg tatcggtcag ccggtcacta tcaccaccga tatttacggc
     7381 gatgatgtga aatacaccgg taaagtggtt ggtctggata tgggcacagg tagcgcgttc
     7441 tcactgcttc cggcgcaaaa tgcgaccggt aactggatca aagtcgttca gcgtctgcct
     7501 gttcgtatcg aactggacca aaaacagctg gagcaatacc cgctgcgtat cggtttatcc
     7561 acgctggtga gcgtcaatac cactaaccgt gacggtcagg tactggcaaa taaagtacgt
     7621 tccacaccgg tagcggtaag caccgcgcgt gaaatcagcc tggcacctgt caataaactg
     7681 atcgacgata tcgtaaaagc taacgctggc taatccagag gtgcgtgtga tgcaacagca
     7741 aaaaccgctg gaaggcgcgc aactggtcat tatgacgatt gcgctgtcac tggcgacatt
     7801 catgcaggtg ctggactcca ccattgctaa cgtggcgatc cccactatcg ccgggaatct
     7861 gggctcatcg ctcagccagg gaacgtgggt aatcacttct ttcggggtgg cgaatgccat
     7921 ctcgatcccg cttaccggct ggctggcaaa gcgcgtcggg gaagtgaaac tgttcctctg
     7981 gtccaccatc gcctttgcta ttgcgtcgtg ggcgtgtggt gtctccagca gcctaaatat
     8041 gctgatcttc ttccgcgtga ttcaggggat tgtcgccggg ccgttgatcc cgctttcgca
     8101 aagtctattg ctgaataact acccgccagc caaacgctca atcgcgctgg cgttgtggtc
     8161 gatgacggtg attgtcgcgc caatttgcgg cccgatcctc ggcggctata tcagtgataa
     8221 ttatcactgg ggctggatat tcttcatcaa cgtgccgatt ggcgtggcgg tggtgttgat
     8281 gacattgcag acgctgcgcg gacgcgaaac ccgcaccgaa cggcggcgga ttgatgccgt
     8341 ggggctggca ctgctggtta ttggtatcgg cagcctgcag attatgctcg accgcggtaa
     8401 agagctggac tggttttcat cacaggaaat tatcatcctt accgtggtgg cggtggtggc
     8461 tatctgcttc ctgattgtat gggagctgac cgacgataac ccgatagtcg atctgtcgtt
     8521 gtttaagtcg cgcaacttca ccatcggctg cttgtgtatc agcctcgcgt atatgctcta
     8581 cttcggcgct attgttctgc tgccgcagtt gttgcaggag gtctacggtt acacggcaac
     8641 gtgggcaggt ctggcctctg cgccggtagg gattattccg gtgatcctgt cgccgattat
     8701 cggccgcttc gcgcataaac tggatatgcg ccgactggta accttcagct ttattatgta
     8761 tgccgtctgc ttctactggc gtgcctatac ctttgaaccg ggtatggatt ttggcgcatc
     8821 ggcctggccg cagtttatcc agggcttcgc tgtggcctgc ttctttatgc cgctgaccac
     8881 cattacgctg tctggtttgc caccggaacg actggcggcg gcatcgagtc tctccaactt
     8941 tacgcgtacg ctggcggggt ctatcggcac gtcgataacc acaaccatgt ggaccaaccg
     9001 cgagtcgatg caccatgcgc agttaaccga gtcggtaaac ccgttcaacc cgaatgccca
     9061 ggcgatgtac agtcaactgg aagggcttgg gatgacgcaa cagcaggcct ccggctggat
     9121 tgcccagcag atcactaacc aggggctgat tatttccgcc aatgagatct tctggatgtc
     9181 agccgggata ttcctcgtcc tgctggggct ggtgtggttt gctaaaccgc cctttggcgc
     9241 aggtggcggc ggaggcggtg cgcactaagt acaactaagc cagttcattt gaactggctt
     9301 ttttcaatta attgtgaaga tagtttactg actaaatgtg cagttcctgc aacttctctt
     9361 tcggcagcgc cagttcttcg ttgctgttga tgcgcacgtc acgttccaga atgctacgcg
     9421 caatatcctg cgcttcctgc aacgagtgca tctggtaagt gccacactgg tagacgttca
     9481 gctccggaat ctgattctga tcctgcactt tcagcacgtc ttccattgcc gctttccagg
     9541 catcagcaac acgctgctca tctggcgtac caatcagact catataaaaa ccggtgcggc
     9601 agcccattgg cgagatatcg ataatctcta caccattacc gttaagatgg ttacgcataa
     9661 aaccagcaaa caggtgctcc agggtatgga tccctctttc tggcatcact tctttgttcg
     9721 gcacgcagaa gcgcagatcg aacacggtga ttgcgtcgcc atgcggggtg ttcattgttt
     9781 tcgccacccg aactgcaggc gcttccatcc gggtatgatc gactgtgaag ctatctaaca
     9841 acggcattta gccacctccg gtaatttttt taaaaatttt ctgaactctt tcttcccagg
     9901 cgagtctgag tatatgaaag acgcgcattt gttatcatca tccctgaatt cagagatgaa
     9961 attttggcca ctcacgagtg gcctttttct tttctgtcag gcgtgttttt ccagccacac
    10021 cgcaaacggt tcggtatctg cggcttccat ttcctgctga cggcgttcag acgcctcgcg
    10081 ctcggctaca aaatcctctt cgcgcagaat ttccagcggc tcttcacgca gcagattacg
    10141 gtaggcttct gcaaatgctt tgcctgttcc gccaatacca gtatcaatca tagaccttaa
    10201 gatacgggca gagaaagtca gatcgggatt atcgaagcag gcgaccagtt catcacacac
    10261 tttctgatac gcttcgccgc cgttaatgct atccagcgtt tgcgcgacgc gtttcagatc
    10321 gcggaacaga tctttaccca cctgcggtaa cgggaactgc gcggtttcgc agccgatacc
    10381 cagcgtcaga cccggtttgc gaccttcgag gatcacccgg ttccagttaa cgcgtgtaca
    10441 ggcaagttcg ctactgctca tttccggtgc atcagccagc gcacaccaga ccataaacag
    10501 gtcgaggaat cgcacctgct gttcatctac accaatcggc gagaacgggt tgatgtccag
    10561 cgaacgcact tcaatatatt caatgccgcc acgtaacagc gcatcagaag gcgactcgcc
    10621 gctgcgggta acgcgttttg gacgaatcgg cgcgtacagt tcgttttcaa tctgcagcac
    10681 attgctattg atttgcagcc gcttaccatc tttctcaata ccaatcttcg cgtactcttc
    10741 cgatggcgtt ttgattgcct gtttcaggcc cgctacgtac tcgtaaagat cgttgaaggt
    10801 aataccaaga ttgctttgcg atttattggt atagccaaga tcgctcaaac gaagagaggt
    10861 cgcatacggc aggtaataca taccgcactc ggttttctca aacggcagcg acgttggttt
    10921 accttgcagg aatgaagagc atattgccgg agacgcgcca aacagataag gaatgaccca
    10981 accgaagcga tagtagttgc gaataacgcg gaaatagccc gcagagattt tctctttagc
    11041 atcagcgccc gaaatatcgc cacacttcgc ttgccagaat gccataggca aagagaagtt
    11101 gtaatgcacg ccggagatgg tttgcatcag cgcgccgtag cgatttttca gcccttcacg
    11161 gtacagcgtt ttaaagcgtc cggtgttaga agtgccatac tgcgccagtt cgatgtcctg
    11221 accttctgcg atgtagcatg gcatacttaa cggccacatc cgctcatcgc ccatgttgcg
    11281 cgccgtatag cgatgcaggt cgcgcataaa ggtcagcata tgttcaatat caccatccac
    11341 tggtgtaata aattccagca atgcttccgc aaaatcggtg gttatccatt tatgcgtcag
    11401 tgcggaacct aatgcttcag gatgacctgt tgttgccagt gtgccatcag cattaacacg
    11461 caaagtttcg cgctccagcc cacgctgtat cccctttaac gcctgaggat gtttttccag
    11521 ccaggccagc gcctgtgata cgtccgggat caaattgacc tcccgcctgt caaaatcgtt
    11581 ttaattagca taactgtaat ggtgaccata tgtgcaggcc tacaattagt gccaccacat
    11641 catgccctga acggtcgctg ctgcaactgc aacatagcgt aacgctttac caaggcacaa
    11701 aaaaaagatt accggccccc aggagatgcg catccatccc gctaacaggc acagtaaatc
    11761 gccaactacg ggcatccagc ttaataatag cgtgactgca ccatagcgtt tcagccagcc
    11821 agtagctttc tcttgccagc gcgatgtttt acgcaatgga aagaaacgcc caaggataac
    11881 gttagttaac cctccaaggc tattacccat tgttgctgtt aaaactaaaa cccagggatg
    11941 actgatcccg gaaagcaacg ttgccaccag cacgacttcg gagttgccgg gtaatagtgt
    12001 agcgctgaga aaactactgg caaacaacga gaaaagcgat aacgcttcac tcacagcaag
    12061 cgaacatcca cagcgtccat gcctgctgca cgggccgcct gaataccgaa atcggcatct
    12121 tcaaagacca cacactgcgt cggttgcacg cccatacgct gcgcgcacaa caaaaatgtg
    12181 tctggcgcgg gtttatggtg tttgacgtga tcggcagcga cgacggcgtc aaaataacgg
    12241 cgtaatccca ggtgagccag caatgcctca gcgatggcgc tttcactccc cgttcctaca
    12301 gccattgggc gacgaccatg ccaacttttc accacttcaa caagaggaag cggctcgaca
    12361 ctatccagca gcatacttct tactgcttct gttttttcac gcgctaacgc atgcgggtcg
    12421 agatcggcct gattcagctc aataattgcc tgagcaatac gccaggtggg cgatccatta
    12481 agcgcaatca tcgcctgaac atcgtactga agaccgtagt gccctaatac ttcgcgccac
    12541 gctttacggt gcgtaggctc cgtatccagg attgtgccat ccatatcaaa aattaaacct
    12601 gcataacgct cgtacatggt cttctcgcaa gtcgaaaaat atgacgctac tttagcgtaa
    12661 ttgcttgatt ttgtcgctga tggcgaagga gaagaaaatt caggaaatgg ataaagtagt
    12721 tatttagaaa tgagatattt ttgaaggaaa ttttttggag
//
LOCUS       DAZGBC010000146        12493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGBC010000146 DAZGBC010000000
VERSION     DAZGBC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.147"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            143..1657
                     /gene="caiT"
                     /locus_tag="ACHK1J_003944"
     CDS             143..1657
                     /gene="caiT"
                     /locus_tag="ACHK1J_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO7913152.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1688..2830
                     /gene="caiA"
                     /locus_tag="ACHK1J_003945"
     CDS             1688..2830
                     /gene="caiA"
                     /locus_tag="ACHK1J_003945"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO7913153.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2959..4176
                     /gene="caiB"
                     /locus_tag="ACHK1J_003946"
     CDS             2959..4176
                     /gene="caiB"
                     /locus_tag="ACHK1J_003946"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO7913154.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4250..5803
                     /gene="caiC"
                     /locus_tag="ACHK1J_003947"
     CDS             4250..5803
                     /gene="caiC"
                     /locus_tag="ACHK1J_003947"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO7913155.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPKFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5912..6697
                     /gene="caiD"
                     /locus_tag="ACHK1J_003948"
     CDS             5912..6697
                     /gene="caiD"
                     /locus_tag="ACHK1J_003948"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO7913156.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVDSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6703..7293
                     /gene="caiE"
                     /locus_tag="ACHK1J_003949"
     CDS             6703..7293
                     /gene="caiE"
                     /locus_tag="ACHK1J_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO7913157.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGHDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRTVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVAPKR"
     gene            complement(7412..7807)
                     /gene="caiF"
                     /locus_tag="ACHK1J_003950"
     CDS             complement(7412..7807)
                     /gene="caiF"
                     /locus_tag="ACHK1J_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO7913158.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8068..11289)
                     /gene="carB"
                     /locus_tag="ACHK1J_003951"
     CDS             complement(8068..11289)
                     /gene="carB"
                     /locus_tag="ACHK1J_003951"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO7913159.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11307..12455)
                     /gene="carA"
                     /locus_tag="ACHK1J_003952"
     CDS             complement(11307..12455)
                     /gene="carA"
                     /locus_tag="ACHK1J_003952"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO7913160.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETAIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2912 a         3269 c         3320 g         2992 t
ORIGIN      
        1 acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc cacgaggtta
       61 ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa ataaatattc
      121 gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa tagaaccgaa
      181 ggttttcttt ccaccgttaa taatcgtcgg catactttgt tggcttacag tcagagatct
      241 ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg tatggggatg
      301 ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg tgtttggccc
      361 gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca gttggatctt
      421 tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga ttgagatcta
      481 ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag cgaaagagtt
      541 ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt acagcttcct
      601 ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc gccccagctc
      661 gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta ccatcgtcga
      721 caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc
      781 gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc aactggacgc
      841 tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcctgcg gtctgcaaaa
      901 aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt
      961 gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg tggggatgtt
     1021 gctgatgtat ctgccgcgaa tgttgttcta taccgatccc atcgctaaag gcggcttccc
     1081 gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc agatgagtat
     1141 cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg gcatggtgat
     1201 ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca ctctgctgtt
     1261 gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc
     1321 catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg gcttcttcat
     1381 cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc tggcgatgtc
     1441 tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta tcggttggtc
     1501 aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga aaccgattca
     1561 aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg tgacgctctc
     1621 ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa tatcaagagg
     1681 ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg gtatccgcga
     1741 actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg acagcgtcta
     1801 cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc tgatccctga
     1861 agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga tggagctggg
     1921 acgtctgggg gcaccaacct atgtactgta ccagttgccg ggcggattca acaccttcct
     1981 gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca ccggtaagca
     2041 gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta gcctgaaaac
     2101 gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt ttattaccag
     2161 cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc
     2221 tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga ccaaacttga
     2281 gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg tggaactgga
     2341 cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag aagagttcga
     2401 ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg cctttgaaga
     2461 tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc gtttccagtt
     2521 gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa acatgctgta
     2581 tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag cgatgtgcaa
     2641 atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg tgctgggcgg
     2701 tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc gtgtagaccg
     2761 cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg tgctgaagca
     2821 ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca ggcctacggg
     2881 agcacgaatg taggccggat aaggcgttta cgccgcatcc ggcagtcatg cgctacataa
     2941 atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc gctggccgga
     3001 ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca aatgttcgca
     3061 gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac cattcgcgtt
     3121 caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc
     3181 aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa
     3241 gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact gtggcagcac
     3301 aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac cgaggagtac
     3361 accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct gattcagaac
     3421 ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt ttctggcctg
     3481 accgccacca cggcggcgct ggcagcactg cataaagcgc gtgaaaccgg taaaggcgaa
     3541 agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt catgatggat
     3601 tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc ctactacgcc
     3661 ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt gggcataacc
     3721 caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac accggaaatc
     3781 ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact ggtagaagaa
     3841 aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg ctttgccgaa
     3901 ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa tccacagtat
     3961 gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg caaagggccg
     4021 aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat gccctcacat
     4081 ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga cattcaggag
     4141 ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag tcggcttgcc
     4201 tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa tggatatcat
     4261 tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg gtcataaaac
     4321 ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc ttgagttaaa
     4381 tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca aaggcgacaa
     4441 ggttgcacta catctcgaca actgcccgaa atttatcttt tgctggttcg ggctggcaaa
     4501 aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa gcgcgtggat
     4561 cctgcaaaat agccaggcgt gcctgctggt gacaagtgcg caattctatc ctatgtatca
     4621 acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag atgtggcact
     4681 tcccgctgat gatggcgtga gttcgtttac tcaactgaaa aatcaacaac ctgccacctt
     4741 gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca cctccggcac
     4801 cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg ctggatatta
     4861 ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac ctgacggtaa tgcctgcgtt
     4921 tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg ccacctttgt
     4981 gctggtcgag aaatacagcg cccgcgcctt ctggggacag gtgcagaagt accgcgccac
     5041 cattaccgaa tgtattccga tgatgattcg tacgttgatg gtacagccgc cttcagcgaa
     5101 cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg agcaggaaaa
     5161 agatgcgttt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga tgacggaaac
     5221 cattgtgggc attatcggcg atcgccctgg cgataaacga cgctggccgt cgattggtcg
     5281 ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc tcccggcagg
     5341 tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca aagagtattt
     5401 tctcaacccg aaagccactg caaaagtgct ggaagctgat ggctggctgc ataccggcga
     5461 taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct gcaatatgat
     5521 caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg ccacccatcc
     5581 gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg aagccatcaa
     5641 agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt ttcgcttctg
     5701 cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa aagatctgcc
     5761 acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac ggcagggaac
     5821 taccctgcca gctaataatc acgctctaaa acaatatgca ttttttgcag ggatgttgtc
     5881 gtccctgaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc tgacccgcaa
     5941 tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg atgcaaaaac
     6001 cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat tacgtgtcgc
     6061 cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa aagcggcagc
     6121 agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat taaccgaaat
     6181 tttcaatctc gacaaaccgg ttatcgcagc tgtgaacggc tacgcctttg gcggcggttt
     6241 cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct tcgccctgcc
     6301 ggaagccaaa ctgggcatcg ttcctgacag cggcggcgtg ctgcgtctgc cgaagatcct
     6361 gccgcctgcc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg cagaagaggc
     6421 gctgcgttgg ggagtagtca accgcgtggt tagccaggcg gaactgatgg ataacgcccg
     6481 cgaactggcg cagcaactgg ttgacagcgc cccgctggcg atcgcagcgc tgaaagagat
     6541 ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc gcagcggcgt
     6601 gttgaaacac tatccatcag ttctgcattc agaagatgcc attgaagggc cgctggcgtt
     6661 tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta ttacgccttt
     6721 gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc cgtcttgatt
     6781 ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact gcgtggtgac
     6841 tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat tatgcatggc
     6901 tactgcgaca ctgacactat cgttggggaa aacggccata tcgggcacgg agcgatcctg
     6961 catggttgtg tgattggtca cgatgcattg gtcgggatga acagcgtgat tatggatggt
     7021 gcggtcattg gcgaagagag cattgttgcc gccatgagct ttgtcaaagc gggctttcgc
     7081 ggcgagaaac gtcagttgtt gatgggtacg ccagcccgcg cggtacgcac tgttagtgac
     7141 gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt cgggcgctgt
     7201 catgcatcgt tacatgaaac acagccactg aggcaaatgg aggaaaatcg cccccgtttg
     7261 caggggacga cggatgtggc gccgaaacgg taatcttgcc ggatgcggcg taaacgcctt
     7321 atccaactct gctggagagc aaccgtaggc cggataagat gcgccagcat cgcatccggc
     7381 gaccaggtcg cacggtatca tcgaaatgac attaacgacg catactcttt gacaacatca
     7441 tctgccactt ctgctcgcgg ttaagttccg tgagcggaac ggcaggaata cgcggtgtct
     7501 ttcttacgcc gactaatttt tcccgactgt tattgcgcag gcgcgcgtaa atttgctcat
     7561 cgatatcgac cactttaacc agtcgctgac actggcatcc ccgcccttcc agcttattag
     7621 ggatcatctt aacttcgcag cttatttctg tgacttccga cagaatataa gtcagggtat
     7681 taaccgcctt gctgtgttca atatcgaaat gaatagagat ctctcttgct atcacccacc
     7741 gattttcagc catcatccat tcggcgatta acaagtagag tggtttttca acatatcctt
     7801 cacacatacg aactccagtt tcacaaatta aaagtctggc tatgactcgt tttgaagcaa
     7861 ttaaacaagg ccatattaaa attgtggata tatcgatagt atgcaacttc accgtcgata
     7921 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttcccgaaaa gatttactta
     7981 atgggcaaaa aaaacggtcc tcatcagaga accgtattag ttattcgatc aaattagcgg
     8041 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
     8101 aattactttt tcggtcgcat cggcattcag cgccatcgcg gtggcaaagc cgccgttcag
     8161 ggtggtgtcg taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
     8221 aatcgcgcga cgacctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
     8281 gtcctgaatg tgcggacgac cttcatgcac cttgtttacc agacgcggat tgatacccgc
     8341 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
     8401 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcagcgcacg
     8461 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
     8521 ggtgcggccc acgcccatga cttccccggt agagcgcatt tctggcccta acagcgggtc
     8581 aacgcccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
     8641 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
     8701 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
     8761 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcaggcc
     8821 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
     8881 ctgacttaag gtgtacgctg gcagcgaaca tgcggagtca ccggagtgca cgcccgcctg
     8941 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     9001 gtcaacttct actgcgtcat caaggaaatg atccagcaac actggcgcat cgttagacac
     9061 gctaaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     9121 ccgaccgccg agaacgtaag acggacgcac caccagcggg tagccaatct cttttgcctt
     9181 ctcaactgcc atttcaatag cagtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     9241 gtcaaccgca tgctggaagc gttcacggtc ttccgcacgg tcgatagcat ccgggctggt
     9301 gccgataacc ggtacgccag cggcttccag tgcacgcgcc agtttcagcg gggtctgacc
     9361 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     9421 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtag agacggtttc
     9481 cgggttacag ttaaccataa tggtttcgta accgtcttca cgcagcgcca gcgaggcgtg
     9541 tacgcagcag tagtcgaatt cgataccctg accgatacgg tttggaccgc cgccaagcac
     9601 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     9661 catgtaagcg gtgtcggtgg caaactctgc cgcacaggta tccacgcgct tgtagaccgg
     9721 gtgcaggtca tactggtcac gcagcttacg gatttccgct tcgcgtacgc ccgccagttt
     9781 tgccaagcgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     9841 gccagtgatg cccacttccg ccactttctc ttccagacgc accagttctt caatctgtac
     9901 caggaaccag cggtcaatgt tagtcaggtt gaagacgccg tccacagaca ggcccgcgcg
     9961 gaaagcatcg gcgatgtacc agatacgctc tgcgcctgcg tctttcagtt cgcgacggat
    10021 tttggttaac gcttccgggt catccaggct cactttcggg tcgaatccag tcgcaccgac
    10081 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
    10141 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccggcga atttttcgaa
    10201 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
    10261 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
    10321 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgcgacac
    10381 gcgcgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
    10441 gttggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
    10501 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg aatcaccagt
    10561 gtggatgccc atcgcatcga agttttcgat agagcagacg atgatgcagt tgtcgttttt
    10621 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcaa
    10681 ctctttggtc ggagagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
    10741 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
    10801 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
    10861 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
    10921 aatcgcatcg gcagtggcac caatcatggt gacgccgaac tcttccaaca cgccctgacg
    10981 ttccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atggttggca gcaccgcgtc
    11041 cgggcgctct ttttcaataa tcttgcgtac cacttcccag tgaatcggct cgatgtaggt
    11101 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
    11161 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
    11221 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
    11281 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
    11341 tcgataaagt ggtcgaacaa cggcgcggcg tcgtgtggac cagggctggc ttcagggtga
    11401 ccctggaagc tgaacgccgg tttatcggtg cgatgaatgc cctgtaacgt accgtcgaac
    11461 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
    11521 tggttctggg cggtgatcat caccacgttt ttctcaacat ctttaaccgg atggttgccg
    11581 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
    11641 tgaccgaggc agatgccgaa taccggaata gcggtttcga ggaatttctg gatggcggta
    11701 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
    11761 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
    11821 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
    11881 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
    11941 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccgttcaga
    12001 cctgggaacg cgcgggcttt ttctaacgcc agcgccgcat ccgggttatc gcctgcgata
    12061 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgtg tcagcttacg ggtatcgata
    12121 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag ataggtcttc ggtattacgg
    12181 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
    12241 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
    12301 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
    12361 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
    12421 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
    12481 actttatttg cat
//
LOCUS       DAZGBC010000147        12463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGBC010000147 DAZGBC010000000
VERSION     DAZGBC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.148"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..532
                     /gene="trmL"
                     /locus_tag="ACHK1J_003953"
     CDS             59..532
                     /gene="trmL"
                     /locus_tag="ACHK1J_003953"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO7913161.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYSAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(585..1406)
                     /gene="cysE"
                     /locus_tag="ACHK1J_003954"
     CDS             complement(585..1406)
                     /gene="cysE"
                     /locus_tag="ACHK1J_003954"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO7913162.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(1486..2505)
                     /gene="gpsA"
                     /locus_tag="ACHK1J_003955"
     CDS             complement(1486..2505)
                     /gene="gpsA"
                     /locus_tag="ACHK1J_003955"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO7913163.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(2505..2972)
                     /gene="secB"
                     /locus_tag="ACHK1J_003956"
     CDS             complement(2505..2972)
                     /gene="secB"
                     /locus_tag="ACHK1J_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO7913164.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(3035..3286)
                     /gene="grxC"
                     /locus_tag="ACHK1J_003957"
     CDS             complement(3035..3286)
                     /gene="grxC"
                     /locus_tag="ACHK1J_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO7913165.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(3427..3858)
                     /locus_tag="ACHK1J_003958"
     CDS             complement(3427..3858)
                     /locus_tag="ACHK1J_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO7913166.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            4103..5647
                     /gene="gpmM"
                     /locus_tag="ACHK1J_003959"
                     /gene_synonym="pgmI"
     CDS             4103..5647
                     /gene="gpmM"
                     /locus_tag="ACHK1J_003959"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO7913167.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKNRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVDEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            5681..6940
                     /gene="envC"
                     /locus_tag="ACHK1J_003960"
     CDS             5681..6940
                     /gene="envC"
                     /locus_tag="ACHK1J_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO7913168.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            6944..7903
                     /locus_tag="ACHK1J_003961"
     CDS             6944..7903
                     /locus_tag="ACHK1J_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO7913169.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPNAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(7890..8924)
                     /gene="waaH"
                     /locus_tag="ACHK1J_003962"
     CDS             complement(7890..8924)
                     /gene="waaH"
                     /locus_tag="ACHK1J_003962"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO7913170.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNHGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(9163..10188)
                     /gene="tdh"
                     /locus_tag="ACHK1J_003963"
     CDS             complement(9163..10188)
                     /gene="tdh"
                     /locus_tag="ACHK1J_003963"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO7913171.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(10198..11394)
                     /gene="kbl"
                     /locus_tag="ACHK1J_003964"
     CDS             complement(10198..11394)
                     /gene="kbl"
                     /locus_tag="ACHK1J_003964"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO7913172.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(11669..>12463)
                     /gene="yibB"
                     /locus_tag="ACHK1J_003965"
     CDS             complement(11669..>12463)
                     /gene="yibB"
                     /locus_tag="ACHK1J_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO7913173.1"
                     /translation="NKGFREKLARSVDVYFSYFERLAVLENEMIIFTSPDLKPRVEAI
                     RNGKPTTVIAIDIKKKFRHIRSRIEKIQKDESFTNRLEPRQLKNPEYWSPEYVLVCNL
                     KAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDENKMHLFTIKKGLT
                     VTSQQQAFDFMIGNHVYIIGGAIVGSQYKWKEFYKLVLESQKITLNNNIVDDDQGIFV
                     MCYYKRSGLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         3182 a         3294 c         3090 g         2897 t
ORIGIN      
        1 aaaaggccgc acggttcacc aattttctgc tatcctgccc ccgcactaag ggggcagtat
       61 gctaaacatc gtactttacg aaccagaaat tccgccaaat actggcaaca tcatccgtct
      121 ttgcgctaat accggctttc gtctgcatat catcgaaccg atgggatttg cctgggacga
      181 taagcgcctg cgccgcgcgg ggctggacta tcacgagttt accgccgtta cgcgtcatca
      241 tgactacagc gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa
      301 aggtacgcct gctcacagcg ccgtaagcta tcaggatggc gattatctga tgttcggccc
      361 ggaaacacgc ggcctgccag cgagcattct tgatgccctg cccgctgaac aaaaaattcg
      421 cattccgatg gtgccagaca gccgcagcat gaacctgtcc aatgcggtgt cggtagtggt
      481 gtatgaagcc tggcggcagc tgggatatcc gggagcggtg ttgagagatt agttacgcca
      541 tgccggatag atgattacat cgcatccggc acgatcacag gacatcagat cccatcccca
      601 tactcaaatg tatggttaat accgttgaaa tgctggtcca tatccattga tggcttatcg
      661 ctgtctggtt taccgacaat gcgtgccgga acgccagcgg cggtggtatg cggcggcacc
      721 ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg
      781 agaatcttcg cgcccgcgcc aatcatcacg ccttcacgaa ttttcgggtg acggtcgccg
      841 ctagttttcc cagtaccgcc aagggtgacg gattgcagaa tcgacacgtc gttttcaatc
      901 accgccgttt cacccacaac gatacccgtg gcgtggtcaa gcatgatacc gcgaccaatt
      961 tttgctgccg ggtgaatatc aacctggaac gtcacagaaa cctggttttg cagaaagatt
     1021 gccagtgcgc gacgcccctg attccacaac cagtgaccga tgcgataggc ctgcaaggca
     1081 tgaaaaccct tcaggtataa caacggggtt gagtatttat cgactgccgg gtcgcgggta
     1141 cgcaccgcct gaatatcaca ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct
     1201 tcttccacca cttcacggat agcaatagca ggcataattg gcgatgacag cttgttcgcc
     1261 agcatgtagc tcagtgcact gccaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa
     1321 ctggccagca ttggctcaca gtccgccagc gttctggctt cggctttaat attgttccag
     1381 acaatttcca gttcttcaca cgacattgct tactccacac gatgagataa tgaccggccc
     1441 gttctgcgcg ggccgggtca tagcggtaac aaaggttccc tggggttagt ggctgctgcg
     1501 ctcgtccttg cgtgcacgac ctaataacgt caatgctgcc tcgcgcgcgt tttttccgca
     1561 atataatact tgataaattt cctcggttat tggcatttca acgccgaagc gatgcgccag
     1621 ttcgcggact tctttcgtat tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc
     1681 gctttgtaca tccatgccct gaccgagcat catgccaaaa cggcggttac gcgactggtt
     1741 gtcggtacag gtaagcacca gatcgcccag ccccgccatg cccataaagg tggcaggatc
     1801 ggcacccagc gccgcgccaa gacgcgacat ttcagccagg ccacgggtga ttagcgccgt
     1861 acgcgcattc gcgccaaaac cgataccgtc ggacatcccc gcgccaatgg caatgacgtt
     1921 tttcaccgcg ccgccaagct gcacgccaat gaaatccggg ttactgtaaa cgcggaaact
     1981 tttaccgcag tgcagcagct gctgaagatc atcagcaaaa gtttggtcgg tcgaggccag
     2041 cgaaatggct gtcggtaaac ctgccgccag ttctttcgca aacgttgggc cagagataac
     2101 cgccagcgga atttgatcgc ctaacgcctc gcgggccacg tcctgtaaca gacgcccggt
     2161 ttccgcttcc agccctttgg tcgcccacac cagacgcgca tcaggacgca tcagcggttt
     2221 aatctggcgc agcacttcac caaagacatg gctgggtacg acgacgagaa tattacggct
     2281 ggctgccagc gcagtggcga gatcgctttc aagatggagt gtatcgggaa aaggcacatc
     2341 ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg
     2401 gccccagagg acaacctcgt ggccatttct tgccagggta atggcaagag cggtgccgta
     2461 cgagccggca ccgatcacag tcattgaagc attacgttgg ttcatcaggc atcctgatgt
     2521 tcttcagtac cttcgccagc ctgctgctgc aaatagttca tgaacagcgc atcgaagtta
     2581 accggcgcaa ggttcagttg cgggaatgta ccgcgggata ccatgctggt gatgcactca
     2641 cgagcatacg ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc catctgggtg
     2701 ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa cttcacacag gaacgcggtt
     2761 tcttcgccca gggaggccgt tacggtaaca cgcagtacca cttcgtaaac gtcatctgcc
     2821 agttgagtag atgccgtatc cagatcaagt ttaacttctg gctgccaatc tttctggaaa
     2881 acgtgcggcg cgttcggcgc ttcgaaagaa atatccttgg tataaatacg ttggatctgg
     2941 aaagtcattt cggtgttgtt ttgttctgac atgtgtagaa aacccttaag tgttgtcctt
     3001 aaatactgcg caatgtaatg ccaacgttca cacgttattt cagcagggga tccagtccac
     3061 cacgtgcatc caatgcatac aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa
     3121 tctggggaac cgtggtgcga ccgctgcgtt tgatcatctc ttcacgctta gcggcgttgc
     3181 catcgatcgg caactcctga aaactcacgc ccttgctgct cagcagtgct tttgcacgat
     3241 ggcaatacgg gcaggtttct ttggtataga tttcaacatt ggccataact tagctcctgt
     3301 tttttaccct gaaatttcat gttgcagggc ggcagcaaaa tggagtgtcc ccagaagctt
     3361 ataacaataa gtcgctgggg gcctaattgc agctaacggc ctgcatcatg aaagacgaca
     3421 ggtgaattat ttgccgcgca ccaaaggcaa gttttcgcca gcccagccag cgacgccttc
     3481 tttcagcacg aatacttgcg caaaaccagc tttcgtcagt gcgtttgcag gctcctggca
     3541 ctgcatgcca gaaccgtcta ccacgataac cggtttgtct ttgtgcttct caagctcacc
     3601 aacattgttg gctttgattt cgctcggcaa cagattaata gaacctgcga tatggccttt
     3661 acggaagtca tcacgctgac gtaaatccac aaccacagcg tcttctttgt tgatcagacg
     3721 cgtagcttca ccacgagtaa tcaccttcac tttcgaggtc aggctcttaa acgtagtcac
     3781 aagaaccgcc accagtaacg cgatccaggc gatactcagt atgggatgac ggccaacaaa
     3841 ttgcataatt tcttgcatgg ggggtaacaa ctcccgacgt agtgattaaa aaaaccagga
     3901 aaggagtata cctgcgcggt gcggcaaata cagccagcgc gttaactgga atgcaatttt
     3961 gcggggcgcg acgaaaaaaa agcgtaaatc tggcatcggc ttgccgcgag cggtcgtata
     4021 ttttgatctt cagaggctat tttatcgatt cagctgtagt aaaattacgc aaattttgac
     4081 tcttgagtat gaggttgtcg caatgtcggt ttctaaaaaa cctatggtac tggtgattct
     4141 ggatggctat ggctatcgcg aagaacagca ggataacgcc atttttagtg ctaaaacccc
     4201 ggtaatggat gcactgtggg ccaatcgtcc gcataccctg atcgacgctt ccggtctgga
     4261 agtcggtctg cctgaccgtc agatgggtaa ctccgaagta ggccacgtta acctgggtgc
     4321 cggccgcatc gtgtatcagg acctgactcg tctggacgtt gaaatcaaaa atcgcgcttt
     4381 ctttgctaat ccagtgctga ctggcgcggt agataaagcg aaaaacgcag gcaaagcggt
     4441 acacattatg ggtctgctct ccgcaggcgg cgtacacagc cacgaagatc acatcatggc
     4501 aatggtagaa ctggcagctg aacgcggcgc agaaaaaatc tacctgcacg cattccttga
     4561 cggtcgcgac actccgccgc gcagtgctga atcctcgctg aaaaaattcg aagagaaatt
     4621 tgccgcgctg ggtaaaggcc gcgtagcgtc tatcattggt cgttactacg cgatggaccg
     4681 cgataaccgt tgggatcgcg ttgaaaaagc ttatgacctg ctgactctgg cgcagggtga
     4741 gttccaggcc gataccgccg ttgctggttt gcaggctgct tatgctcgtg acgaaaacga
     4801 cgagttcgtg aaagcgaccg ttattcgtgc agaaggtcag ccagatgcgg caatggaaga
     4861 cggcgatgcg ctgattttca tgaacttccg tgctgaccgt gcgcgtgaaa tcactcgtgc
     4921 tttcgtgaac gctgatttcg atggcttcgc gcgtaagaaa gtggttaacg tcgatttcgt
     4981 gatgctgacc gaatacgccg ctgacatcaa aactgcggtt gcttacccac ccgcttccct
     5041 ggttaacacc ttcggcgagt ggatggcgaa aaacgacaaa actcagttgc gtatttccga
     5101 aaccgaaaaa tatgcccacg ttactttctt ctttaacggt ggcgtagaag agtcgttcaa
     5161 aggcgaagat cgcattctga tcaactcacc gaaagtggct acctacgatc tgcaaccgga
     5221 aatgagctcc gcagagctga ccgaaaaact ggttgcggca atcaagagcg gcaaatacga
     5281 caccattatc tgtaactatc cgaacggcga catggtaggt cacaccgggg tgatggaagc
     5341 agcagttaaa gccgttgaag cgctggatca ctgcgtggat gaagttgcga aagcggttga
     5401 gtctgtaggt ggacaactgc tgatcaccgc tgaccacggt aacgctgaac agatgcgcga
     5461 tccggcaacg ggtcaggcgc acacagcgca caccaacctg ccagttccgc tgatttacgt
     5521 tggcgataag aacgtgaaag cggttgaagg cggcaaactt tctgacatcg cgccgaccat
     5581 gttgtcgctg atgggtatgg aaatcccgca agagatgact ggtaagccgc tgttcatcgt
     5641 ggaataatcc ctccccatga ggggaaaggc gattaatacc atgacacggg ccgtgaaacc
     5701 gcgcaggttt gcaatcaggc ccatcatcta cgccagcgtt cttagcgctg gcgtattgtt
     5761 gtgcgccttt tccgcccacg cggatgagcg tgaccaactc aaatctattc aggccgatat
     5821 cgccgcgaaa gagcgcgcgg tacgccaaaa gcaacaacaa cgcgcaagcc tgctcgcaca
     5881 attgaaaaag caggaagaag cgatctctga agccacccgt aagctgcgcg aaacgcaaaa
     5941 cacgctcaat caactcaata aacagattga tgagatgaac gcgtcgattg ccaaactgga
     6001 gcagcaaaaa gccgcccagg agcgcagcct cgccgcacaa ctggatgccg cattccgtca
     6061 gggcgagcat accggtattc agctgattct cagcggtgaa gaaagccagc gtggacagcg
     6121 tttacaggct tatttcggct atctcaacca ggcgcgacaa gaaaccattg cccagttgaa
     6181 gcaaacgcgt gaagaagtcg ccatgcagcg tgctgaactg gaagagaaac agagcgagca
     6241 acaaacgctg ttatatgagc agcgcgccca acaggcgaaa ctgactcagg cgctgaacga
     6301 gcgtaaaaag acgctggcag ggctggagtc ttccatccag caaggtcagc aacagttgag
     6361 cgagctgcgc gccaacgaat cccgtctgcg taacagcatt gcccgtgcgg aagccgcggc
     6421 gaaagcgcgt gcagaacgag aagcacgtga ggcccaggcg gttcgcgacc gccagaaaga
     6481 agcgacgcgt aaaggcacca cctacaaacc gactgaaagc gaaaaatcgc tgatgtcccg
     6541 taccggtggt ctgggcgcgc cgcgtggtca ggcattctgg ccggttcgcg ggccgacgct
     6601 gcatcgctat ggcgagcagc tacagggtga attacgctgg aaagggatgg tgattggtgc
     6661 ctctgaaggt actgaagtta aagcgattgc cgacggtcgg gtgattctgg ctgactggct
     6721 gcaaggctac ggtctggtgg tggtggttga gcatggtaaa ggcgacatga gtctttatgg
     6781 ctataatcag agcgcactgg tgagcgttgg ttcgcaggtt cgcgcgggcc agccaattgc
     6841 actggtgggc agcagtggcg gtcagggtcg gccttcactc tatttcgaaa ttcgccgcca
     6901 gggtcaggcg gtcaatccac agccgtggtt gggaagataa gttttgtttc catttcgtcg
     6961 taacgttctt gcatttgccg ctctgttggc gctctcctcc cccgtaattg ctggcaaact
     7021 tgccatcgtc attgatgatt ttgggtatcg cccgcacaac gaaaaccagg tgctggcgat
     7081 gccttccgct atctccgtcg ctgtattacc cgattcaccg aacgccagag agatggcgac
     7141 caaagcgcat aacagcggcc acgaagtgtt gattcatcta ccaatggcac cattgagtaa
     7201 acagccgctg gagaaaaata cgctacgccc ggagatgagc agcgacgaaa ttgagcgcat
     7261 tattcgtagt gcggtcaata acgtgcccta tgccgtgggg atcaacaacc acatgggcag
     7321 caagatgacc tctaacctgt ttggtatgca gaaagtgatg caggcgctgg agcgttacaa
     7381 tctttacttc ctcgacagcg taaccattgg taatacccag gcgatgcgcg ccgcgcaagg
     7441 cactggcgtg aaggttatta aacggaaggt gttcctcgac gattcacaga acgaagcgga
     7501 catccgtgtg cagttcaatc gcgcaattga tctggcgcgt cgcaacggtt caaccattgc
     7561 cattgggcat cctcaccctt caacggtacg cgtgctacaa cagatggttt ataacctgcc
     7621 gccagacatt acgctggtga aagccagcag cttgctgaat gaaccgcagg ttgatacgtc
     7681 tacaccgccg aaaaacactg tgcccgacac accgcgtaat ccgttccgtg gcgtgaagct
     7741 gtgcaaaccg aagaaaccga tagaacctgt ttatgctaat cgcttctttg aagtattaag
     7801 cgaaagcatc agccagagca cgctgatcgt ttacttccag catcagtggc aaggctgggg
     7861 caaacagccc gaagcggcaa aacttaacgc tagcgcaaat taaaggcgct acgcgtaatg
     7921 cgatggtgcg accgcgtttt gtcgcgccat tgccataagc ggaaagacca cagtaacgcc
     7981 tggtaaccca ccttcacgct gcgcacattg gtaatcaggc gcttatacat accggaagta
     8041 aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg caccgcatgg
     8101 caaacacgca atgcttcgta agttatttgc tgatgaaatt ccggataaat cgtaattttg
     8161 tcggcataat ttcgatttaa tttctccagc aggcgggtaa tcttaatata gtgacgttga
     8221 taattaaggt ttttattccc ttgtctatgc aaccgactca ctgacgtatt atgcagataa
     8281 tatttatata atgattgctc ggtatatcgc gcacgcagcg cgttaaacat gaattctgtt
     8341 gtccagacaa tatcctgatg atgtaatccg gcaataaatt taatgttatt tttaacaata
     8401 acatcgcggc gataaacccc catccagaca acgtgagtcc aacggcgcga agaaagcccc
     8461 atccgcagcc agtccgggcc ggttaataca ccggttgagc gaaggcgatc gctggggatg
     8521 gattgccagg tttctcccgt ttcacgaaaa caccagtcag cgttgcactg cgccacgtcg
     8581 aggtcgtcct ctaacgccat ggtcatcagc gtttcgtaca tggtgggata gacttcatcg
     8641 tcagcatcga caaaagcgac atatttgccc gttgccactt caatcccatg attacgcgcc
     8701 accgatgcgc cagcattcgc ctgatgcaac aaacgaacgt gcggatagtt ttctgcgtaa
     8761 tgctttgcta tttcaacaga attatccgtt gaaccatcgt taataataat gatttccaga
     8821 gcagtccagg tttgcgtaat taaagattcc atacaagtgc ggaaatcatc gcccgcatta
     8881 tataacggaa taataacact aagtttattg gtgctgttca tcataaatta ccgacgacct
     8941 ctgaagttat cctgacgttt tacaggcttt gtattaagaa actattaagc ctgtgagata
     9001 ttctttttac aggtgagaga gtcacttcag ggtagagaga taatgagagc agctatataa
     9061 gagatcagca caattacgaa ctctttatta cactgacagg attttacata tttaacagat
     9121 tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc agctcagaat
     9181 aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat catcgataga
     9241 gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg ccgccatctt
     9301 gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc ccttaaagat
     9361 cacttttgtc cagtctatcg acatgtcaga aggtggaata cccagcatcg caatacgacc
     9421 gccgtggttc atggtgtcga gcatggtacg aaacgctggc ggcgcaccgg acatttccag
     9481 accgacatca aagccttcgg tcatgcccag ttctgccatc acatcattaa ggttttcttt
     9541 agcgacgttg accgcacggg tgatacccat tttgcgcgcc agttcgagac gatattcatt
     9601 aacatcagtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg cagccataat
     9661 acctatcgga cctgcaccag agaccagcac atcctcgcca accagatcga acgacagcgc
     9721 ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg agatattgtc
     9781 gggaattttg aaagcgttga acgccggaat caccagatat tcggcgaagc agcccggacg
     9841 gttaacgccg acgccgatcg tgttgcggca cagatgggta cgaccgccac gacagttgcg
     9901 acagtgaccg caagtgatat gaccttcgcc agaaacgcga tcgccaatct tgaagccttt
     9961 cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga ccatcggcac
    10021 cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag tcccgcagat
    10081 ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta caggaacgtc
    10141 ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct cacatcctca
    10201 ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac gcgtaatttg
    10261 ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt tcggaacgac
    10321 cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctcacggg caaatttctg
    10381 cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc ccgccagggt
    10441 aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca gacggtcacg
    10501 cagctcgcta cccgcttcga ccatctccag tactttgatg gatgcggcaa caatggccgg
    10561 tgccagcgag ttggagaaca gatacggacg agaacgctgg cgcagccact caaccacttc
    10621 tttgcgcgcc gcagtataac caccagaagc cccgcccagc gctttaccaa gcgtaccggt
    10681 gataatatcg acccggccca tcacatcgca gtattcatgg gaaccacgac cgttttcacc
    10741 gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt tatctgccag
    10801 atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac catcggtggc
    10861 aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt caagctcctg
    10921 catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac catcaataat
    10981 agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca gcgtttcaaa
    11041 caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca tccccaggaa
    11101 tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa taaaacgcac
    11161 cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gccgccgcaa tcagatccgg
    11221 atgattcgcc agcccgagat agttattggc acaaaagtta atgacgtggc ttccatcagc
    11281 cacagtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa acaacccttc
    11341 cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac gcattgcgat
    11401 tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc gacgagatga
    11461 cgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt ctctcaacac
    11521 ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt gattttactg
    11581 cattgatata aaaagaaaaa atgatgccaa aacatcattt ttcctatctc agttggcatc
    11641 aaaacgctac ccactaatgg cgggaaacct attttcttga aaggaaaatt ctcagcgcct
    11701 gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac cattttcctc
    11761 tccccagata gtttaggttg aagagaccgg aacgtttgta atagcacatg acaaaaatgc
    11821 cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc agaactaatt
    11881 tatagaattc tttccattta tactgtgaac caacaattgc gcccccaata atatatacat
    11941 gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc ccttttttta
    12001 tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc aatcctcgag
    12061 tcacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc gttttcacta
    12121 agcccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc aaaacatact
    12181 ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt gtaaacgact
    12241 catctttttg aattttttcg atacgacttc tgatatgtct aaatttcttt tttatatcga
    12301 tagcgataac cgttgttggt tttccgttgc gaatcgcttc aacccttggt ttcaaatcgg
    12361 gagaagtgaa aataatcatc tcattttcaa gtacggccag acgctcaaag taactaaaat
    12421 aaacatcaac tgaacgagct agtttttcac ggaacccttt att
//
LOCUS       DAZGBC010000148        12269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGBC010000148 DAZGBC010000000
VERSION     DAZGBC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.149"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            316..1086
                     /gene="yjjP"
                     /locus_tag="ACHK1J_003966"
     CDS             316..1086
                     /gene="yjjP"
                     /locus_tag="ACHK1J_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO7913174.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1077..1550
                     /gene="yjjB"
                     /locus_tag="ACHK1J_003967"
     CDS             1077..1550
                     /gene="yjjB"
                     /locus_tag="ACHK1J_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO7913175.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1657..2196
                     /gene="dnaT"
                     /locus_tag="ACHK1J_003968"
     CDS             1657..2196
                     /gene="dnaT"
                     /locus_tag="ACHK1J_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO7913176.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKTEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2199..2936
                     /gene="dnaC"
                     /locus_tag="ACHK1J_003969"
     CDS             2199..2936
                     /gene="dnaC"
                     /locus_tag="ACHK1J_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO7913177.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2985..3479
                     /gene="yjjA"
                     /locus_tag="ACHK1J_003970"
     CDS             2985..3479
                     /gene="yjjA"
                     /locus_tag="ACHK1J_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO7913178.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3733..6024
                     /gene="opgB"
                     /locus_tag="ACHK1J_003971"
     CDS             3733..6024
                     /gene="opgB"
                     /locus_tag="ACHK1J_003971"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO7913179.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQTVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6162..7184)
                     /gene="lgoD"
                     /locus_tag="ACHK1J_003972"
     CDS             complement(6162..7184)
                     /gene="lgoD"
                     /locus_tag="ACHK1J_003972"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO7913180.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATLEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7323..8237
                     /gene="lgoR"
                     /locus_tag="ACHK1J_003973"
     CDS             7323..8237
                     /gene="lgoR"
                     /locus_tag="ACHK1J_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO7913181.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVMARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8452..9813
                     /gene="lgoT"
                     /locus_tag="ACHK1J_003974"
     CDS             8452..9813
                     /gene="lgoT"
                     /locus_tag="ACHK1J_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7913182.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9862..11526)
                     /gene="tsr"
                     /locus_tag="ACHK1J_003975"
     CDS             complement(9862..11526)
                     /gene="tsr"
                     /locus_tag="ACHK1J_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO7913183.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11754..12269
                     /locus_tag="ACHK1J_003976"
                     /pseudo
     CDS             11754..12269
                     /locus_tag="ACHK1J_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3052 a         3183 c         2932 g         3102 t
ORIGIN      
        1 tgcggaatca cacaatcaat atttattcaa taaaatctga tagttttaac ttgttaacat
       61 taaaaaacgc gcccagtcac ctattatcct gattcgtaaa ggctaaataa tgaattcatt
      121 atagtgataa tattcatgtg ataatttgtt tgcggcgaga gataattcgc tttttatcac
      181 cgagcttctc ccgataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      241 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      301 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacacggct ttgtatccag
      361 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      421 cgactgggtc gggcgctggg aatggacagc gtagaaagtt ctatctcttc gaacgccata
      481 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      541 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      601 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcagccatt acgttaccca
      661 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      721 ggtggctggg atggtgccgt catcaccttc tttgccagta cggccgcgat gtatatccgc
      781 cagctgctgg cacaacgtca tcttcatcca caaatcaact tttgcctcac cgctttcgcc
      841 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
      901 gcgatggccg ccagcgttct gctgctggtt ccgggctttc cgttgattaa tgccgtcgcc
      961 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
     1021 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
     1081 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
     1141 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1201 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1261 caacctttat ggcttcgatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1321 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1381 cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1441 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1501 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1561 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1621 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1681 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaaactgaa
     1741 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     1801 cgcctggctg aactgctggc gctggaagaa aagctggcgc gtccgggaag cgatgtcgat
     1861 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     1921 ttcgccatgt atccggactg gcaacccgat gccgacttta tccgcctggc ggcgctatgg
     1981 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     2041 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     2101 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2161 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2221 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     2281 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2341 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2401 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2461 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2521 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2581 aatccgtatt gatcatcacc gtggccgata ttatgtcagc gatgaaagat accttcagga
     2641 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2701 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     2761 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     2821 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttgt
     2881 gggtgatctt caactgggat agctaccgta gccgggtaac aggtaaagag tattaatccc
     2941 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactgtta
     3001 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     3061 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3121 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccagg
     3181 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3241 agctggcgtc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3301 tgaacagtga agagcaaaaa gaagacacca actatctgga cggcattcag ggtttgctga
     3361 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacaactccg ctggcagaaa
     3421 aggtgaaaac caaagcctgc gatctggtgt taaaacaggg gctgaacttc atttcctgaa
     3481 ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
     3541 ctgctcctgg acggttttac ccctgttaca cgggaagtga ccgtaatcaa aaaagtctgt
     3601 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3661 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3721 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     3781 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacgttaac ggtgctgggg
     3841 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     3901 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     3961 atcctgccgg gtatcggcat tgtgctgggg ctgacggcgg tgttcggtgc gctgggctgg
     4021 atcctgcgcc gtcgtcgtca tcatccgcac cattttggtt acagcctgct ggcactctta
     4081 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
     4141 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4201 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4261 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4321 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4381 gcttctcagt gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc cgcttctgtt
     4441 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4501 aactatttcg tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt cctgaagtcg
     4561 catggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc cgacccgcac
     4621 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4681 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4741 cacccggatg gttttatctc gcgcacctgt aaccgcaaaa aatatgattt tgacggtaag
     4801 ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac gtttatcaac
     4861 aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc tgaccattta
     4921 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
     4981 gtcattcgtg gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa cacgatggat
     5041 aacggcgcga cggtactgga cattctcggt ggcgataact atctcggact tggtcgtagc
     5101 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5161 tggaagccag atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5221 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5281 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5341 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgaccg ttgctacaag
     5401 atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt ctcgcagggg
     5461 cagcttggcg gtcagcaaac tgttcagcat gtcgacaaaa caacatggaa gagcaagacg
     5521 gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga tacgctgaag
     5581 attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt cgccggtgcg
     5641 ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg ccgctggtcc
     5701 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     5761 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     5821 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     5881 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     5941 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     6001 aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata aggtgcgacc
     6061 tacacacctt ctccggctta caacacatta ttaagaaaca aaaaaaacag cgcccaacac
     6121 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacgcct
     6181 ttaattaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6241 ggatagcgat gggttaacat catgtcagca gtgatttttc cttccgccat cagtcgaccg
     6301 actttagcaa aatcttccag cgtggcgttg cggctaccca tcatcgtcgt ttctttttta
     6361 tggaattcag gatcggagaa ctgcaactct cctttaaaca ggccgacaaa taccaccgtg
     6421 ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattgcc tgtcgcgtcg
     6481 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg
     6541 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6601 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6661 ccgattggcc ccgccccaac caccagcacc tgctcgccgg gagcaattgc tgcgcgacgc
     6721 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     6781 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     6841 tgcacgccaa tgactgaaat tttttcacag caattggtac gcccgctttt acacgccggg
     6901 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     6961 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     7021 ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac
     7081 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7141 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt
     7201 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7261 gtgaaaacac acgcataaaa atgttttaaa tcgggtttta atggactcac aaggaggaaa
     7321 atatgagtcg ttcacaaaat ttacgccaca atgtgattaa ccaggtcatt gatgatatgg
     7381 cgcggggtca tatcccttcc ccacttccat cgcaaagtgc gctggcggaa atgtacaaca
     7441 tcagccgcac tacagtgcgt catattctca gccacttacg cgaatgcggc gtcttaacgc
     7501 aggttggaaa cgactatgtt atggcgcgta aacctgacca tgatgacggt ttcgcctgta
     7561 ctaccgcttc aatgagtgaa caaaacaaag tgtttgagca ggcttttttc acgatgataa
     7621 accagcgtca gttacgccct ggggaaactt tttctgaact gcaactggct cgggccgcgg
     7681 gtgtcagccc ggtggttgta agagaatatc ttttaaaatt cggccgttat aatcttattc
     7741 aaagcgaaaa gcgcggccag tggagcatga agcaattcga tcaatcctac gccgagcagc
     7801 tgtttgagct acgcgaaatg ctcgaaacac attcacttca gcactttctc aacctgcctg
     7861 atcatgatcc ccgctggctg caggcgaaaa ccatgctcga acgtcatcgg ctactgcgtg
     7921 acaacatcgg caatagcttt cgcatgttct cgcaacttga cagagatttt cactccttac
     7981 tgctttcagc tgccgacaat atcttttttg atcaatcgct tgagatcatc tcagtcatct
     8041 ttcattttca ctatcaatgg gacgaaagcg atcttaaaca gcgcaatatc attgccgtcg
     8101 atgagcatat gaccattctc agcgctctga tttgccgcag tgaccttgat gccacccttg
     8161 cgctccgtaa ccatttgaat tcagctaaac aatcaatgat tcgatccatt aatgagaaca
     8221 cgcgttatgc tcattaagta cggattaaaa acagcaaact attagcgggt gcacggtttt
     8281 atcaaaagat aaataccggg aaattcccgc ttacctatgc tcacaatcag tcgactaaaa
     8341 atgtttttgt gagcggtagt aaagtcctaa aactttaacc tgccgttaat gtcaaccttg
     8401 cgtaatgcac aattacgtaa acaattaaaa ttataaatac aggagtccgg cgtggaaaaa
     8461 gaaaatatca ccctcgatcc gcgttcttca tttactccat cttcgtcggc agatattccc
     8521 gtgccaccag atggattagt tcaacgcagt acccgaatta aacgcattca aaccaccgcc
     8581 atgttgttat tattttttgc ggcggtaatc aattatctcg accgcagttc gctgtcggta
     8641 gcaaatttaa cgattcgtga agaattggga ttaagtgcca ccgaaatcgg cgctttgctc
     8701 tccgtgtttt cactcgctta cggcattgcg caacttcctt gcggcccact attggatcgt
     8761 aaaggcccac gcctgatgct gggactgggg atgttcttct ggtcactgtt ccaggccatg
     8821 tctggcatgg tgcacagctt tacgcagttc gtgttggtgc gtatcggtat ggggattggt
     8881 gaagcgccga tgaacccatg cggtgtaaaa gtcattaacg actggttcaa catcaaagag
     8941 cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc cgtaagccca
     9001 ccgattctgg cggcgatgat gctggtgatg ggctggcgcg ggatgtttat taccattggt
     9061 gtactgggga tttttctcgc catcggctgg tatatgctct atcgcaaccg cgagcacgta
     9121 gaactgactg ccgttgaaca agcttatctc aatgcaggta gcgtcaatgc ccgccgagat
     9181 ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc gcacaatgtg gggaatgatg
     9241 ctcggattca gtggcatcaa ctacactgcg tggctgtatc tggcttggct tcctggttac
     9301 ctgcaaacag cctataacct ggatttaaaa agtacagggt tgatggcggc tatccctttc
     9361 ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ctgactggct ggtcaaaggg
     9421 ggaatggctc cgattaaaag ccgtaagatc tgcattattg ccgggatgtt ctgttctgcc
     9481 gcctttacgc tgatagtacc gcaagcgaca acatccatga cagcggttct gctgattggt
     9541 atggcactgt tctgtattca ctttgccgga acatcctgct ggggcttgat ccacgtcgca
     9601 gttgcttctc gcatgactgc gtcggtgggc agtatccaga actttgccag cttcatctgc
     9661 gcctctttcg cgccgatcat tactggtttt attgttgata caacccactc attccgtctg
     9721 gcactaatca tttgcggttg cgtcaccgca gcgggagcac tggcgtacat cttcctggtt
     9781 cgtcagccga tcaacgaccc gcgtaaagat taaactccct atgcccgata acattttgct
     9841 tatcgggcat ttttatggcg attaaaacgt ttcccagttc tccccgctat ctgccactgc
     9901 cattttacgc ggcgtagctg gcgtcacggt ttttaccaca gccgatgttt cacgctgctg
     9961 ttgctgctgt tgctgaatcc ggaacactgc cacagcttcg gtcaggcgac tggcctgctc
    10021 ttccagcgcg gcggcggcag cggcagattc ttccaccagc gcggcgttct gttgagttac
    10081 ccggtccatc tcagcaaccg ctaagccaac ctgatcgata ccacggctct gctcatcaga
    10141 agcagaagct atttcgccca taatgtccgt cacgcgggtc acagcactga caatctccgc
    10201 cattgtttcc ccggcgcttt cgaccagcgt agagccaaca tccactttcc ccaccgagtc
    10261 ttcaatcagg cttttaattt cacgagccgc ctgggcgcta cgctgggcca gattacgcac
    10321 ttctcccgca accaccgcaa aaccgcgccc ttgctcaccc gcacgcgcag cctcaaccgc
    10381 cgcgttcaaa gccagaatat tggtctggaa agcaatgccg tcaattacgc tgataatatc
    10441 ggcgattttc tgcgaactgg tggagatatc gcgcatagtc tgcaccacgt tatctaccac
    10501 tttgccgccg cgttgcgccg tttcagaagc acttaacgcc aggtggctgg cctggcgcgc
    10561 attctcggcg ttctgtttca ccgttgcggt cagttgctcc atgctggctg ccgtctcttc
    10621 cagcgaagcg gcctgttgct cggtgcgcga agagagatcg ttattaccgg tggcgatttc
    10681 gctggcaccg ctatagatgg cattggcccc gttgcgcaca tcaccgacgg tacgcatcag
    10741 ctctccctgc atatggcgca aactttctgc cagttgcccc atctcattag agccatccac
    10801 ctcaatcggt ttcaccagat cgccgcctgc aatatgacga atgctgtcaa tcagacgatt
    10861 cattggcgct accagcgagg ctttaatacc gaaccagacg gcgaagatga ccgccagtac
    10921 gacgatcatc acgcccacca gaatccacat cgcctggctg taggaggcat tgttatcgct
    10981 gacggcgata tcatagagcc gatcgttttg ctccatgtaa gccacatact gcttctcgaa
    11041 accgtcctga tatccctggg tcggctgatc aaagaactcg ttgattttgc ctgcacctaa
    11101 cagttggatc agctccgcca gcgcattgtg ataaatatcg taattacgtt tgatctctgc
    11161 cgctgcggcg gtgctctgac gcgggtcacg cggcaacgct tcgtaatctg cccagttttt
    11221 ttccgcctgt ttcagcgaaa tactggcact ctgcatcagc tcagcaacgg ttgaaccgct
    11281 accaatatta ttctgatcca tcatgtagcg gatacccgca cggttgaggg tgttacgcgt
    11341 ctgcaacaac gcgacccagc tgccattcag cgtggattgc tgctggcgaa tggtttgtaa
    11401 aacagtgaaa ttttctttgt cattctttaa ggcattaaag aacagaccgc ctgatgtcag
    11461 ttgtaaaagg ccaaaaacgg ccaaaaccag cagtaagctg gtcacgatct tgatacgttt
    11521 taacatggtt tctctttcct gtggaccgat gcaagatttt cggcctgaaa tgagaaaact
    11581 ttataggtag cgacttgaaa ctggttaatt ttacctcact tttttaattt ttgttcaaaa
    11641 atacaataac cacttattga tacattttct tttcttattt ttttcacacg aattgtatct
    11701 attttgaacg aaatatttta tagagtgtgg aattttaaga aaggacatct gaaatgttaa
    11761 accgtgactt cgatcatctc tcaacgctat tacatctctt ttttgaacgt gaggatattt
    11821 gccaaaagct taatattatt gaccaaaaca atatcaccgg ttgggaagtg tggttccagg
    11881 tagagtttgc taatatgctc tgctcaacag accatgaatg gtggcgtgaa caagcacttt
    11941 cctgtgatat gcgcaaaaaa cctgagcgcc cgacactacg cacagatttc ttactacgta
    12001 aaaaaggctg ggcacaagat agctatatca cgcttgaaat aaaacaaaac agagacgcga
    12061 ccagttgtgt taaaaatatg atcgcagact tagaaaaaag cgcaaaaata aaacgttccg
    12121 aactcgattt acgcagtttc tgaaccttgg gtatcacccc tatgatagac agggaaaggc
    12181 tggactctct catcgattgc tatttagatg agaaatttta tcttactaaa tctcgtaaaa
    12241 aacacgtttt acttaaagaa ataaataac
//
LOCUS       DAZGBC010000149        12231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGBC010000149 DAZGBC010000000
VERSION     DAZGBC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.150"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..506
                     /gene="cybB"
                     /locus_tag="ACHK1J_003977"
     CDS             3..506
                     /gene="cybB"
                     /locus_tag="ACHK1J_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO7913184.1"
                     /translation="MSIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMIHVSCGISIL
                     VLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGLVMMYNRGNP
                     WFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHYFWKDNTLLR
                     MMPRKRS"
     gene            751..924
                     /locus_tag="ACHK1J_003978"
     CDS             751..924
                     /locus_tag="ACHK1J_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913185.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            950..1018
                     /gene="yncP"
                     /locus_tag="ACHK1J_003979"
     CDS             950..1018
                     /gene="yncP"
                     /locus_tag="ACHK1J_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO7913186.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(996..1145)
                     /gene="hokB"
                     /locus_tag="ACHK1J_003980"
     CDS             complement(996..1145)
                     /gene="hokB"
                     /locus_tag="ACHK1J_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO7913187.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            1544..3184
                     /gene="trg"
                     /locus_tag="ACHK1J_003981"
     CDS             1544..3184
                     /gene="trg"
                     /locus_tag="ACHK1J_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO7913188.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRVGGMFMIGAFVFALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITTVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVLAEPRGAGKPVSFATV"
     gene            complement(3223..4146)
                     /gene="ydcI"
                     /locus_tag="ACHK1J_003982"
     CDS             complement(3223..4146)
                     /gene="ydcI"
                     /locus_tag="ACHK1J_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7913189.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            4363..5706
                     /gene="ydcJ"
                     /locus_tag="ACHK1J_003983"
     CDS             4363..5706
                     /gene="ydcJ"
                     /locus_tag="ACHK1J_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7913190.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5931..7586
                     /gene="opgD"
                     /locus_tag="ACHK1J_003984"
                     /gene_synonym="mdoD"
     CDS             5931..7586
                     /gene="opgD"
                     /locus_tag="ACHK1J_003984"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO7913191.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7726..7950
                     /gene="ydcH"
                     /locus_tag="ACHK1J_003985"
     CDS             7726..7950
                     /gene="ydcH"
                     /locus_tag="ACHK1J_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO7913192.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            8013..8552
                     /gene="rimL"
                     /locus_tag="ACHK1J_003986"
     CDS             8013..8552
                     /gene="rimL"
                     /locus_tag="ACHK1J_003986"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO7913193.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(8544..9524)
                     /gene="ydcK"
                     /locus_tag="ACHK1J_003987"
     CDS             complement(8544..9524)
                     /gene="ydcK"
                     /locus_tag="ACHK1J_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO7913194.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGKSR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            9648..10640
                     /gene="tehA"
                     /locus_tag="ACHK1J_003988"
     CDS             9648..10640
                     /gene="tehA"
                     /locus_tag="ACHK1J_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO7913195.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            10637..11230
                     /gene="tehB"
                     /locus_tag="ACHK1J_003989"
     CDS             10637..11230
                     /gene="tehB"
                     /locus_tag="ACHK1J_003989"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO7913196.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            11534..12202
                     /gene="ydcL"
                     /locus_tag="ACHK1J_003990"
     CDS             11534..12202
                     /gene="ydcL"
                     /locus_tag="ACHK1J_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO7913197.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATSKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         3146 a         2842 c         3068 g         3175 t
ORIGIN      
        1 aaatcagcat tcactggctg gtctttttgc tggttatcgc agcgtattgc gcaatggagt
       61 ttcggggttt cttcccacgt agcgatcggc cactcatcaa catgattcat gtttcctgcg
      121 gcatatcaat tctcgtgctg atggtcgttc gtcttttgtt aaggctgaaa tacccaaccc
      181 cgccgattat acctaagcca aaaccgatga tgacgggact ggcgcatttg ggacatttgg
      241 tgatttatct cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccgag
      301 gcaacccgtg gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgaac
      361 gggtagatag cttaaagtcg tggcatgaaa cgctggcgaa tctgggatat tttgtcatcg
      421 gtttgcacgc tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac
      481 gcatgatgcc gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg
      541 acccgtggta caggcgaaga atacgggtct acatcggaag cgcctattat atttatttgt
      601 atgacaaata aaaccataat ccttgcccat acgtccatct ggcttatttt taatcaattc
      661 acccgatctt tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcga
      721 acataagatt cgataaacca tggaggttat atgaaaaaac tggcacttat tttgtttatg
      781 ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg
      841 gggatctcac actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgca
      901 tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct
      961 agtcagatgc gcggggaaaa tccccgcgct tgcccttacc tggacgtgca ggccatgagc
     1021 gcagcaacct ccttatcacc gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg
     1081 agtgtgaatg tcagaatcgt aatgcagata atgagcagac acaccaccag agggttgtgc
     1141 ttcatagcct ttctccttgc cggatggcgg gtaagaggct aagatctgaa ttgctaggtt
     1201 cattcgttgg cctcggttga tagaaatatc ggtcggggcc ttcgtctttc tgattcccgg
     1261 ttagcctgaa aacagaaagt ctcaggcacc cgtgagcatc ctatgtggtt tccttaggga
     1321 cgaaaataat cacctgacga aatttcgtgc tgttttccag aatttttcgt cattcgggtt
     1381 agccagttta accattcgtt actctcttca ttccaatggc attaatcttc tctgcaataa
     1441 ttgttgtaaa aatgtgacgc aaagaggctt ttagtcgtaa gtaattaccg tcaaatgccg
     1501 atgactttct atcaggagta aacctggacg agagacaacg gtaatgaata caactccctc
     1561 acagcgatta ggttttttgc atcacatcag gttggttccg ttatttgcct gcattctagg
     1621 cggtatctta gttctattcg cattaagttc agccctggct ggctatttcc tctggcaggc
     1681 cgatcgcgat cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag
     1741 ttcagatttt ttgcgttcag cccggatcaa tatgattcag gccggggctg cgagtcgtat
     1801 tgcggaaatg gaagcaatga agcgaaatat tgcgcaagcc gagtcggaga ttaaacagtc
     1861 gcagcaaggt tatcgtgctt atcagaatcg gccggtgaaa acacctgctg atgaagccct
     1921 cgacactgaa ttaaatcaac gctttcaggc ttatatcacg ggtatgcaac ctatgttgaa
     1981 atatgccaaa aatggtatgt ttgaagcaat tatcaatcat gaaagtgagc agatccgaac
     2041 gctggataat gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc
     2101 caaccaactg gcggaactgg cccatcagcg cacccgcgtg ggtgggatgt ttatgattgg
     2161 cgcgtttgtg tttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt
     2221 cattcgtcca ctgcaacatg ccgcacaacg gattgaaaaa atcgccagtg gcgatctgac
     2281 gatgaatgat gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca
     2341 aatgcagcat tcactgggga tgacagtagg gactgttcga cagggcgcgg aagagattta
     2401 tcgtggcacc agcgaaattt ccgctggcaa tgcggacctt tcatctcgca ccgaagaaca
     2461 agcggcggca attgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca
     2521 gaatgcggat aacgcgcatt atgccagtaa actggcgcaa gaggcttcta ttaaagccag
     2581 cgatggcggg cagacggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc
     2641 gaagaaaatt tctgagatca ccaccgtcat caacagtatt gctttccaga cgaatattct
     2701 ggcactgaat gctgccgttg aagctgcgcg agcgggtgag caagggcgtg gatttgccgt
     2761 tgtcgccagc gaagtacgga cactcgcaag ccgcagcgcc caggcggcga aagagattga
     2821 aggcttgatc agtgaatcag tcaggttaat tgacctggga tcggatgagg tggcaacggc
     2881 cgggaaaacc atgagcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga
     2941 aatcgccgcc gcctcggatg agcaaagtag aggcataact caggttagcc aggcgatttc
     3001 tgaaatggat aaggtgacgc aacagaatgc ttctctggta gaagaggcct cagcggcggc
     3061 ggtgtccctt gaagaacagg cggcacgatt aactgaggcg gtggacgtat tccgtctgaa
     3121 caaacattct gtgttggcag aacctcgcgg agcgggtaaa ccagttagtt tcgctacggt
     3181 gtgaaaatgt tcaaggaagg gatcgacaga tcccttcacc tttcagaacg gcattgattt
     3241 tcgaatagcg ttaatcatca actggcaacc agaagagaac gtcgcatcta cgcgggtcag
     3301 tattccaatc ggttcgcctg caccatgtcc cggaacaggc agggccacca gcgtggcatg
     3361 acgcaggtcg tcttttacag cgccagaagg gacaaaccac acgtaatcgt attcaaccgt
     3421 aagttgacgg gatagcgaag cagacagcgt ttcgatacaa cccgaaggaa ttttacatcc
     3481 ctggctctgt actaatgcat ctgaatgctg gcgtggtgca gtgccttctg gtgatacaac
     3541 gaccggccat tccagcactc ggcttagcgt tacgttctcc tgaagtagcg ggtgattagg
     3601 gcggacaacc agcttcagcg attcaagaaa cagcagttcg taattaagcc cggtcatcag
     3661 ttcaggatct gacatccgac caatgccgat atcgatttcc ccggttttta aacccgccag
     3721 aatcataggg ttactcattg tcgcaacttg caaggtcgtc tctttttgtt gctgatgaaa
     3781 ctgacctata accgaaggta atatccccag tgccgcagta ggtagtgcac caaccctgac
     3841 gacatcatta ttaagacctt ctttacgatg aagcgactgt ccagcagtgt tgatggcgtc
     3901 aagaactctg actgcatgcg ttaaaaattg ttcgccgggt aaggtaagtt gcgccccctg
     3961 acgaccacgc tcaaacaagc gagcgcccgt cagttgctcc agttcattca atgtcttaga
     4021 gagcgcaggt tgactcaaat taagggtttc agccgcgcgc cccaaagttc cttgttgtgc
     4081 gacagctacg aatgtatgaa ggtggcgcaa acggatgcgc tgactaaaca gactattttt
     4141 ttccataagc gatgttaaaa acgaagcggt gtcgctgaca agtgaagttg tttgattatg
     4201 ataacttgat tgcaaaatat tattaacaat taaagcaatt atgttacagc aaaatggata
     4261 atattgatgt tttcgcggcg agatcacagt ttgtaaattc ttcccgccag agtgaatgcg
     4321 gttacctaca ctccagatta ctgaccactg gaggcagaca ctatggcgaa cagcatcacg
     4381 gcggatgaga ttcgggaaca gttttcgcag gcaatgtcag ccatgtacca gcaagaagtt
     4441 ccgcaatatg gcacgctgct ggaactggta gctgatgtga atctggctgt gctggaaaac
     4501 aatcctcaac tgcacgaaaa aatggtaaat gcagacgagc tggcgcgact gaatgttgaa
     4561 cgtcatgggg cgattcgcgt tgggactgca caagagcttg ctactcttcg gcggatgttt
     4621 gccattatgg ggatgtaccc ggtgagctat tacgatctct cgcaggcagg ggtgccggta
     4681 cattcgacag catttcggcc cattgatgat gcttctctgg cgcgtaatcc cttccgcgtt
     4741 tttacctcct tactccgcct tgagcttatc gagaacgaaa ttttgcgcca gaaagcggcg
     4801 gagattctgc gtcagcgcga tatcttcacc ccacgttgtc gacaactgtt agaggaatat
     4861 gagcagcagg gcggttttaa cgaaacacag gcacaggagt ttgtgcagga agccctggaa
     4921 acgtttcgct ggcaccagtc agcaacggta gatgaagaaa cctatcgcgc cttgcacaac
     4981 gaacatcggt tgattgctga tgtggtctgt ttccctggat gccatatcaa ccacctgacg
     5041 ccacgtacgc tggatattga ccgggtgcag tcgatgatgc ctgaatgcgg gattgaaccc
     5101 aaaattctga tcgaagggcc gccgcgccgc gaggtgccga ttttactacg gcagaccagc
     5161 tttaaggcac tggaagagac ggtgttgttt gcggggcaga aacagggcac gcataccgcg
     5221 cgctttggtg aaattgagca gcgtggcgtg gcattaacgc cgaaagggcg acaactgtat
     5281 gatgatcttc tgcgtaacgc tggaaccggg caggataatc tcactcacca aatgcattta
     5341 caggaaacct tccgcacttt tcctgacagt gagtttttaa tgcgtcagca agggctggca
     5401 tggttccggt accgtctgac gccttcaggt gaggcgcatc gtcaggctat tcatcccgga
     5461 gacgatccac agcccttaat tgaacgtggt tgggtcgcgg cgcaacccat tacctatgaa
     5521 gatttcttgc ccgttagcgc ggcggggatc ttccagtcaa atctgggtaa tgaaacgcag
     5581 gcacgcagcc acggtaatgc cagtcgcgaa gcatttgagc aggcgttggg ttgtccggtt
     5641 ttggatgagt tccagcttta tcaggaagcg gaagagcgca gtaaacgtcg ctgtggtttg
     5701 ctttaaattc tgaccatccg cctttgcaaa aatttgcctg attttacaaa cgaatcaggc
     5761 tcatcccatc gacataaaaa aattgccgat ttatgcatat tctctgagtt caacaattgg
     5821 attattaata aatattgtct agagtgagcg gtcataaata agcactttct tgccgctgaa
     5881 aacgaccagc gcgggaccat tcacaacacc agaaggactc actttcaggt atggatcgta
     5941 gacgatttat taaaggttca atggctatgg ccgccgtgtg cggtaccagc ggcattgctt
     6001 ctcttttttc tcaggcggca ttcgcggcag attctgatat tgccgacggg caaacccagc
     6061 gttttgactt ctccattcta cagtcaatgg cgcacgactt agcgcaaaca gcgtggcgtg
     6121 gtgcgcctcg tccgttacct gacacgctgg cgacaatgac gccgcaggct tataacagta
     6181 ttcaatacga cgccgaaaaa tcgctctggc ataacgttga gaaccgtcaa ctggacgctc
     6241 agttcttcca tatgggaatg ggattccgtc gccgcgttcg tatgttttct gtagatccag
     6301 caacacatct ggcgcgtgaa attcactttc gcccggagtt gttcaaatac aacgatgcag
     6361 gtgttgatac caaacaatta gaagggcaaa gcgatctcgg ctttgccggt tttcgcgtgt
     6421 ttaaagcccc cgaactggcg cgtcgtgatg tagtatcatt cctcggtgcg agttatttcc
     6481 gcgccgttga tgacacatat caatacggtt tgtcggctcg cggcctggcg atcgacactt
     6541 acaccgacag taaagaagaa ttccccgact ttaccgcctt ctggtttgat acggtaaaac
     6601 cgggggcaac tacctttacc gtttatgcgt tgctcgatag cgccagtatt actggtgcct
     6661 ataagttcac tatccattgt gagaaaagtc aggtgattat ggatgtggaa aatcacctgt
     6721 atgcgcgcaa agacattaaa cagctgggca ttgcgccgat gaccagtatg ttcagctgcg
     6781 gtactaatga acgtcggatg tgcgacacca ttcatccgca aattcatgac tctgatcgtc
     6841 tgtccatgtg gcggggcaac ggcgagtgga tttgccgtcc gctgaataat ccgcaaaaat
     6901 tgcagttcaa tgcttacacc gacaacaacc cgaaagggtt tggtttattg caactggatc
     6961 gtgacttctc ccattatcag gacatcatgg gctggtataa caaacgccca agtctgtggg
     7021 tggaaccgcg taacaagtgg ggtaagggca ccatcggcct gatggaaatc ccaacaacgg
     7081 gcgaaacgct ggataacatt gtctgcttct ggcagccaga aaaagctgta aaagcgggtg
     7141 atgagtttgc attccagtat cgtctgtact ggagtgcgca accgcctgtt cattgcccat
     7201 tagcgcgcgt tatggcgacg cgtaccggca tgggcggttt cccggaaggt tgggcaccag
     7261 gtgaacacta tcccgaaaaa tgggcgcgtc gttttgccgt cgatttcgtt ggtggtgatc
     7321 tgaaagctgc cgcaccaaaa ggcattgagc cggtgattac gctttccagt ggggaagcga
     7381 agcaaatcga aattctctat attgaaccca ttgatggtta tcgtattcag tttgactggt
     7441 atccgacttc ggactccact gatccggtcg atatgcggat gtatctgcgt tgtcaggggg
     7501 acgctatcag tgaaacatgg ctgtatcagt atttcccgcc agcgccggat aaacgtcagt
     7561 atgttgacga ccgcgtgatg agttaatcgt tttttcttcg gcaccttctt cgggaggtgc
     7621 cgtctggtta aacacgatcc cgctcgcatt tttccctaag ttaaatgagt aatctgatgg
     7681 tgtgtatttc agatacacct tgtcagccac taacagggag tgcgtatgtt tccagaatat
     7741 cgagatttaa tatcccgtct gaaaaacgaa aatcctcgct ttatgtcctt gttcgataaa
     7801 cacaataaac ttgatcatga aattgccaga aaggaaggtt ccgacggtcg agggtacaat
     7861 gcggaagtgg tccgcatgaa aaaacaaaag ctacagttaa aagatgagat gctcaaaatc
     7921 ctgcagcatg agagcgtcaa agaggtgtaa actttcctaa gccgccagcc aggcggcttt
     7981 tttaacaact gcatggattg actggagata agatgactga aacgataaaa gtaagcgaat
     8041 cacttgaatt acatgctgtt gcagaaaatc acgtcactcc tctttatcag ttaatctgta
     8101 aaaataaaac ctggttacag cagtcgctaa actggccgca gttcgttcag agtgaagagg
     8161 acacgcgaaa aacggtgcag ggtaatgtga tgttgcatca acgcggctat gccaaaatgt
     8221 tcatgatttt caaagaagat gaacttatcg gcgttatctc gtttaatcgt attgaaccac
     8281 tgaataaaac cgctgaaatt ggctactggc tggacgaatc tcatcagggg caggggatca
     8341 tttctcaggc gctgcaggca ttgattcatc attacgctca gtctggtgaa cttagacgct
     8401 tcgtgatcaa atgtcgggtg gacaatccgc aaagcaacca ggtcgctttg cgcaatggtt
     8461 ttatccttga aggttgcctg aaacaggctg agttcctgaa tgatgcctat gatgatgtga
     8521 acttatacgc gcgtattatc gattcacaat aaccctgcca gcggcgttcg cgtaatgcgc
     8581 tcctcgccgt taatgacttt tggaccacgg acatgaaccg tgtcgccatc aaaggcttca
     8641 acgacggcgt ggtcggttaa ctccacatgg ttttcaatta tcaccgcacc tgtaatacgg
     8701 ctcttacctt gtattacaac gtgctcgtcg agcagaatcg gtccgccacg taccacggca
     8761 tttccgccaa tcaagacatg atgttttaac acacaattac cttccacaat ggcatattcc
     8821 gccacctgcg aactgtaatg aatcgtcgga atggcatctt cttcaatgcc agctttcacc
     8881 tgcgcgtgac cgtagacttt agcgcaatcg catagccaga cgttgttctc ttcattacct
     8941 tcaatactgg caaaatcaaa aacttcggcg cgatgttcaa taaaagcata ccggatgacg
     9001 gcatcgccat aaatttgtgc ctggtggaca atacgcgagg cgctaaccct tgcgcgatca
     9061 taaatttgta gcaggagttg atggtcaggc gttaagcctt gtgctgcgac gatcatagaa
     9121 tgttgattaa ttaaggcgtg accaaatatt ctacattgcc cgcaaaccag tgagtcatga
     9181 atggtgacac tgtcacttat atatgcaccc tggctgattt cactattatc gatccagaca
     9241 ttatccgttg cgtaaacctc tccccacagc acgctggtgc cggtgatacg cgtattaccg
     9301 gaaatcaccg ttccgccgaa cgcaatagca ttctggtcgt aaatccagca atttccttcc
     9361 tgtgctaaca ccgtttcgcg atcgatccag ccgccagcgg ttcctgctat cacatcgtta
     9421 aaatcgctaa tggcgataat ctgccgtaat aacacacttt ttttagtgcc atcttcctga
     9481 taactaaagg ctcgctgttc ttcacttaga cgatatttgc gcatagcgtt ttcccacagg
     9541 tgacttacta taaccgtagc aaattctgcg gctctggcta tgctcgagaa aattccataa
     9601 aatgcatttc aaatatactt tataaattaa acaaaatgag taagaagatg cagagcgata
     9661 aagtgctcaa tttgccggca ggctactttg gtattgtgtt ggggacgata gggatgggat
     9721 ttgcctggcg ctatgccagc caggtttggc aggtcagcca ctggttaggg gatgggttgg
     9781 tgattctggc gatgatcatc tggggattat tgactagcgc atttattgcc cgactcatac
     9841 gctttccgca tagcgtgctg gcggaagttc gccatccagt gctgagcagt tttgtgagtt
     9901 tgtttccggc aacgacgatg ctggtggcga ttggttttgt tccgtggttt cgcccactgg
     9961 cggtgtgcct gttcagtttt ggtgtcgtgg ttcagttggc ttatgccgcc tggcaaactg
    10021 cgggattatg gcgcggatct caccctgaag aagctaccac gcctggactg tatctgccga
    10081 cagttgccaa caactttatc agcgcaatgg cctgtggtgc gttgggctac accgacgccg
    10141 gtctggtgtt tttaggcgca ggcgttttct catggctaag ccttgaaccg gtgatcttgc
    10201 agcgtctgcg tagttcggga gaattaccca cggcactgcg gacatcactc ggcattcagc
    10261 tcgctcctgc gctggtggct tgtagtgcct ggctgagcgt caacggcggc gagggtgaca
    10321 cgctggcgaa aatgcttttc ggttatggac tgctgcaact gctgtttatg ctacgtctga
    10381 tgccatggta tctctcccag ccatttaatg cttcattctg gagtttctcg ttcggcgtat
    10441 ctgcactggc aaccaccggt ttgcatctgg ggagtggcag cgataatgga tttttccata
    10501 cgctggcggt gccgctgttt atctttacca attttattat tgcaatactg ctcatccgta
    10561 cttttgcgct tctgatgcag ggaaaattgt tagtcagaac cgagcgcgcc gttttaatga
    10621 aagcagagga caaagaatga tcattcgtga cgaaaactat tttactgata aatatgaatt
    10681 aacccgcaca cactctgaag tactggaagc ggtgaaagtg gttaaaccgg gtaaaacgct
    10741 ggatctgggc tgtggcaatg gtcgtaacag tctttacctg gcagccaatg gttatgatgt
    10801 tgacgcatgg gataaaaatg ccatgagtat cgccaacgtc gagcgcatta aatctattga
    10861 aaatctggat aatttacaca cccgggtcgt tgatctgaat aacctcacat ttgctggaca
    10921 gtacgatttt attctttcga ctgtggtgct gatgttcctt gaggctaaaa ccatcccagg
    10981 gctgattgcc aatatgcaac gttgcactaa acctggcggt tacaacctga ttgtggcggc
    11041 gatggatacc gctgattatc catgtaccgt cggcttcccg tttgccttca aagaaggaga
    11101 attacgtcga tattacgaag gctgggagat ggtgaaatac aatgaagacg tcggcgagct
    11161 gcaccgcacc gacgccaacg gtaatcgtat taaactgcgt ttcgccacga tgctggcacg
    11221 taaaaaatga cccggtaaga acaaagcgcg tgaaaattcc ccacgctgag atggtttact
    11281 gtccttcttt tcggtaagca tattttttat cgaagggatg tgaaattaat cacagtagtc
    11341 gaagtttttt agcagcttaa cttactgaaa tttaactact gatgattgac ttagcccctt
    11401 ttgcggcatt gactatgtcg tctgaaaagg ggctaaaaaa atttattttc accaacactt
    11461 tttttgccac aacacgaagc ggcgcttttt gctataactt aaaaagtaat ataatcatct
    11521 caggaaacta ttcatgcgta ccacatcatt tgcgaaagtt gcagctttat gcggcttgtt
    11581 ggctctgtct ggttgtgcct ctaaaatcac ccagccagat aaatattctg gttttttaaa
    11641 caattactct gatttaaaag aaacaacctc agcaacaagt aaacctgttt tacgttgggt
    11701 agacccgagt tttgatcaaa gcaaatatga cagcatcgtc tggaacccaa tcacttatta
    11761 tccggtacca aaaccttcta cccaggtagg gcagaaagtt ctggataaaa ttttgaacta
    11821 taccaacacc gaaatgaaag aagcgatagc gcagcgtaaa ccagtggtta ccaccgctgg
    11881 gccgcgtagt ctgattttcc gtggggccat taccggtgta gataccagca aagaagggct
    11941 gcaattctat gaagtggttc ctgttgcatt agtggttgcg gggacgcaaa tggctacagg
    12001 ccaccgtacc atggatactc gcctctattt tgaaggtgag ctgattgatg cagcgactaa
    12061 taaaccggtt atcaaagtcg ttcgtcaggg cgaaggtaaa gacctgaata acgaaagtac
    12121 gccaatggct ttcgaaaata ttaaacaagt tattgatgac atggcgaccg atgccaccat
    12181 gtttgacgtt aacaaaaagt agttccagac gcgccatcgt tagatggcgc t
//
LOCUS       DAZGBC010000150        11999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGBC010000150 DAZGBC010000000
VERSION     DAZGBC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.151"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..202
                     /locus_tag="ACHK1J_003991"
     CDS             <1..202
                     /locus_tag="ACHK1J_003991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913198.1"
                     /translation="RNHASEFTDLIILLLIYFEKGIGIYAVFLKILIIGMDFRARKGF
                     YMDTLLIISSSHHQQFTSLFRV"
     gene            296..2719
                     /gene="pgaA"
                     /locus_tag="ACHK1J_003992"
     CDS             296..2719
                     /gene="pgaA"
                     /locus_tag="ACHK1J_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO7913199.1"
                     /translation="MYSSSRKRCPQTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQTRGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2728..4746
                     /gene="pgaB"
                     /locus_tag="ACHK1J_003993"
     CDS             2728..4746
                     /gene="pgaB"
                     /locus_tag="ACHK1J_003993"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO7913200.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFRWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSPLIEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     AGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVTQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLINQIKSIPQAKDKSILELQAKNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4739..6064
                     /gene="pgaC"
                     /locus_tag="ACHK1J_003994"
     CDS             4739..6064
                     /gene="pgaC"
                     /locus_tag="ACHK1J_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO7913201.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6066..6479
                     /gene="pgaD"
                     /locus_tag="ACHK1J_003995"
     CDS             6066..6479
                     /gene="pgaD"
                     /locus_tag="ACHK1J_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO7913202.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6529..7317)
                     /gene="phoH"
                     /locus_tag="ACHK1J_003996"
     CDS             complement(6529..7317)
                     /gene="phoH"
                     /locus_tag="ACHK1J_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO7913203.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7949..9220)
                     /gene="efeB"
                     /locus_tag="ACHK1J_003997"
     CDS             complement(7949..9220)
                     /gene="efeB"
                     /locus_tag="ACHK1J_003997"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO7913204.1"
                     /translation="MQYEDENGVNELSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLVKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9226..10353)
                     /gene="efeO"
                     /locus_tag="ACHK1J_003998"
     CDS             complement(9226..10353)
                     /gene="efeO"
                     /locus_tag="ACHK1J_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO7913205.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTEADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10411..11241)
                     /gene="efeU"
                     /locus_tag="ACHK1J_003999"
     CDS             complement(10411..11241)
                     /gene="efeU"
                     /locus_tag="ACHK1J_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO7913206.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3201 a         2923 c         2792 g         3083 t
ORIGIN      
        1 ccgaaatcat gcctcggaat ttactgattt aattatttta ctcctaattt attttgaaaa
       61 aggcattggg atttatgccg tatttctgaa gatcctcatc attggaatgg attttcgggc
      121 gagaaaagga ttttatatgg acactctgct catcatttct tcttctcatc atcaacaatt
      181 cacgtctctc ttccgcgttt aataacggat tatgaggtgc aaaaaatatc tttcttttca
      241 gttgcctgtc attagataca gagagagatt ttggcaatac atggagtaat acaggatgta
      301 ttcaagtagc agaaaaaggt gcccgcaaac caaatgggct ttgaaaattc ttactgccgc
      361 atttttagca gcgagtcccg cggcgaagag tgccgttaat aacgcctatg atgcattgat
      421 tattgaagct cgcaagggaa atactcagcc agctttgtta tggtttgcac aaaaatcagc
      481 actcagtaat aaccaaattg ctgactggtt acagattgcc ttatgggccg ggcaagataa
      541 acaggttatt accgtttaca accgctaccg ttatcagcaa ttaccagcgc gtggttatgc
      601 agctgtcgcc gtcgcttatc gtaacctgca acaatggcaa aactcgctta cactgtggca
      661 aaaggcgctc tctctggagc cgcaaaataa ggattatcaa cggggacaaa ttttaaccct
      721 ggcagatgcg ggtcactatg atactgcgct ggttaaactt aagcagctta actctggagc
      781 accggacaaa gccaatttac tcgcagaagc ctatatctat aaactggcag ggcgtcatca
      841 ggatgaatta cgggcgatga cagagtcatt acctgaaaat gcctctacgc aacaatatcc
      901 cacagaatac gtgcaggcat tacgtaataa tcaacttgct gccgcgattg acgatgccaa
      961 tttaacgcca gatattcgcg ctgatattca tgccgaactg gtcagactgt cgtttatgcc
     1021 tacgcgcagt gaaagtgaac gttatgccat tgccgatcgc gccctcgccc aatacgctgc
     1081 attagaaatt ctgtggcacg ataacccaga ccgcactgcc cagtaccagc gtattcaggt
     1141 tgatcatctt ggcgcgttat taactcgcga tcgttataaa gacgttattt ctcactatca
     1201 gcgtttaaaa aagacggggc aaattattcc gccctgggcg caatattggg ttgcgtcggc
     1261 ttatctcaaa gaacatcagc caaaaaaagc acagtcaata atgacagagc tcttttatca
     1321 caaggagacc attgccccgg atttatccga tgaagaactt gcggatctct tttacagcca
     1381 cctggagagt gaaaattatc ccggcgcgct aactgtcacc caacatacca ttaatacttc
     1441 gccgcctttc cttcggttaa tgggcacgcc tacgagcatc ccgaatgata cctggttaca
     1501 ggggcattcg tttctctcaa ccgtagcaaa atatagtaat gatcttcctc aggctgaaat
     1561 gacagccaga gagcttgctt ataacgcacc aggaaatcag ggactgcgca ttgattacgc
     1621 gagtgtgtta caaacccgcg gttggcctcg tgcagcagaa aatgaattaa aaaaagcaga
     1681 agtgatcgag ccacgtaata ttaatctgga ggttgaacaa gcctggacag cattaacgtt
     1741 acaagaatgg cagcaggcag ctgtcttaac gcacgatgtt gtcgaacgtg aaccgcaaga
     1801 tcccggcgtt gtacgtttaa aacgtgcggt tgatgtacat aatcttgcag agcttcgtat
     1861 cgctggctca acaggaattg atgccgaagg cccggatagt ggtaaacatg atgtcgactt
     1921 aaccaccatc gtttattcac caccgctaaa tgataactgg cgcggttttg ctggattcgg
     1981 ttatgccgat ggacaattta gcgaaggaaa agggattgtt cgcgactggc ttgcgggtgt
     2041 tgagtggcgg tcacgtaata tctggctcga ggcggagtac gctgaacgct ttttcaatca
     2101 tgagcataaa cccggcgcgc gcctgtctgg ctggtatgat tttaatgata attggcgtat
     2161 tggttcgcaa ctggaacgcc tctctcaccg cgttccatta cgggcaatga aaaatggtgt
     2221 tacaggcaac agtgctcagg cttatgttcg ctggtatcaa aatgagcggc gtaagtacgg
     2281 tgtctcctgg gctttcactg atttttccga cagtaaccag cgtcatgaag tctcaattga
     2341 gggtcaggaa cgcatctggt cttcaccata tttgattgtc gatttcctac ccagtctgta
     2401 ttacgaacaa aatacagaac acgatacccc atactacaac cctataaaaa cgttcgatat
     2461 tgttccggca tttgaggcaa gtcatttgtt atggcgaagc tatgaaaata gctgggagca
     2521 aatcttcagc gcaggtgttg gtgcctcctg gcaaaaacat tatggcacgg atgtcgtcac
     2581 ccaactcggc tacgggcaac gcattagctg gaatgacgtg attgatgctg gcgcaacgct
     2641 acgctgggaa aaacgacctt atgacggtga cagagaacac aacttatacg ttgaattcga
     2701 tatgacattc agattttaag gataaatatg ttacgtaatg gaaataaata tctcctgatg
     2761 ctggtgagta taattatgct cactgcgtgc attagccagt caaaaacatc atttataccg
     2821 ccacaggatc gcgagtcttt actcgccgag caaccgtggc cgcataatgg ttttgtggcg
     2881 atctcatggc ataacgttga agacgaagct gccgaccagc gttttatgtc agtgcggaca
     2941 tcagcactgc gtgaacaatt tgcctggctg cgcgagaacg gttatcaacc ggtcagtatt
     3001 gctcaaattc gtgaagcaca tcgaggagga aaaccgctac cggaaaaagc agtagtgctg
     3061 acttttgatg acggctacca gagtttttat acccgcgtct tcccaattct tcaggccttc
     3121 cggtggccag ctgtatgggc ccccgtcggc agttgggtcg atacgccagc ggatgaacaa
     3181 gtaaaatttg gcgatgagat ggtcgatcga gaatattttg ccacgtggca acaagtgcga
     3241 gaagttgcgc gttccccgct cattgaggtc gcttctcata catggaattc tcactacggt
     3301 attcaggcta atgccaccgg cagcttattg cctgtatatg taaatcgtgc atattttact
     3361 gaccacgcac ggtatgaaac cgcagcagaa taccgggaaa gaattcgtct ggatgctgta
     3421 aaaatgacgg aatacctgcg tacaaaggct aaggtaaatc cacacgtttt tgtttggcct
     3481 tatggcgaag cgaatggcat agcgatagag gaattaaaaa aactcggtta tgacatgttc
     3541 ttcacccttg aatcaggttt ggcaaatgcg tcgcaattgg attccattcc gcgagtatta
     3601 atcgccaata atccctcatt aaaagagttt gcccagaaaa ttattaccgt acaggaaaaa
     3661 tcaccacaac ggataatgca tatcgatctt gattacgttt atgacgaaaa ccgccagcaa
     3721 atggatcgca atattgatgt gctaattcag cgggtgaaag atatgcaaat atcaaccgtg
     3781 tatttgcagg catttgctga tcccgatggt gatgggctgg tcaaagaggt ctggtttcca
     3841 aatcgtttgc taccaatgaa agcagatatt tttagtcggg ttgcctggca attacgtacc
     3901 cgcgcaggtg taaacatcta tgcgtggatg ccggtattaa gctgggattt agatcccaca
     3961 ttaacgcgag taaaatactt accaactggg gagaaaaaag cacaaattca tcctgaacaa
     4021 tatcgccgtc tctcgccttt tgatgacagg gtcagagcac aagttggcat gttatatgaa
     4081 gatcttgccg gacatgctgc ttttgatggc ctattgttcc acgatgatgc tttgctttca
     4141 gattatgaag atgccagtgc accggctatc gcggcttatc agcaagcagg ctttagcggg
     4201 agtctgagcg aaattcgaca aaacccggag caatttaaac agtggacccg ctttaaaagt
     4261 cgtgcgttaa ctgacttcac tttagaactt agtgcgcgcg taaaagccat tcgtggtcca
     4321 catgttaaaa ctgcacgaaa tattttcgca cttccggtaa cacaacctga aagtgaagcc
     4381 tggtttgcac agaattatgc tgatttccta aaaagctatg actggactgc tattatggct
     4441 atgccttata tggaaggtgt cgcagaaaaa tcggctgatc aatggttaat acaactgatc
     4501 aatcagatta aaagcatccc tcaggctaaa gacaaatcta ttttagaatt acaggcaaaa
     4561 aactggcaga aaaatggtca gcatcaggct atttcttcgc aacaactcgc tcactggatg
     4621 agcctattac aactgaatgg agtgaaaaac tatggttatt atcccgacaa ttttctgcat
     4681 aaccaacctg aaatagacgt tattcgtcct gagttttcaa cagcctggta tccgaaaaat
     4741 gattaaccgc atcgtatcgt tttttatatt atgtctggtg ttatgcatcc ccctatgcgt
     4801 agcgtacttt cactctggtg aactgatgat gaggttcgtt ttcttctggc cgttttttat
     4861 gtccatcatg tggattgttg gcggcgtcta tttctgggtc tatcgtgaac gccactggcc
     4921 gtggggggaa aacgcaccag ctccccagtt gaaagataat ccgtctatct ccattatcat
     4981 tccctgtttt aatgaggaga aaaacgttga ggaaaccata cacgccgctt tagcacagcg
     5041 ttatgagaac attgaagtta ttgccgtaaa tgacggttca acagataaaa cccgtgccat
     5101 cctggatcgc atggctgcac aaattcccca tttgcgggtc attcatctgg cgcaaaacca
     5161 ggggaaagcc attgcgctta aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg
     5221 cattgatggc gacgcgttat tagaccgcga tgcggcggca tatattgtgg aaccgatgtt
     5281 gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt attcgcacac gttctaccct
     5341 ggtgggtaaa attcaggttg gcgagtattc ctcaattatt ggtttgatca agcgaaccca
     5401 gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc
     5461 cctggcagaa gtgggttact ggagtgacga tatgatcacc gaagatattg atattagctg
     5521 gaagctgcag ttgaatcagt ggacgatttt ttacgagcca cgggcgctgt gctggatatt
     5581 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
     5641 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     5701 actgtttttt gaatactgcc tgacgacaat atgggccttt acctgcctgg tcggtttcat
     5761 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     5821 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     5881 gatgattgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     5941 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     6001 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtccc gatcgcggga ttctgagagg
     6061 ttaatatgaa caatttaatt attacgaccc gacaatcgcc agtacgttta ctggttgatt
     6121 atgttgccac aaccatcttg tggacattat ttgctttgtt catattctta ttcgccatgg
     6181 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     6241 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     6301 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     6361 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     6421 tcaccagcca ggggcaaata aaaatggtgg tttcagaaaa agcgctagtc cgggcataaa
     6481 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     6541 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tctttaccga accggacgat
     6601 cccgaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     6661 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     6721 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     6781 gactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcaattt ccaccttccc
     6841 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcacgtc
     6901 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tctccaggta agaagccaag
     6961 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     7021 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     7081 ggcgaatatc agctgcttac tctcaatagc cttcagatag tgcaattgcg cttcatttcg
     7141 cgcgaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     7201 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     7261 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     7321 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     7381 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     7441 acggctacgc gcaccgttta cggcgtcggt aacacatgaa aagaaaggat gaggttgaaa
     7501 atgcaaagtg acgagatgac taccggagga gaaaactccg cgagtggtgg cgcgttgatt
     7561 atctaaaaca tgtccagtac aggacgttac catccgcgat ctccatagtg actgactatc
     7621 actgccggga acttccgctg ctacttaata agtacaacag atctcgcatt cattgcaaca
     7681 atatattcac ttatatttaa ctataaaaca ccattttagt gacattagtt tctactggaa
     7741 agatgacaga gtgatgacag agtgatgaca gagatgaaaa aagctgtgtg ctttcagcag
     7801 gattcacatc aggcatcagt gcacgaacgt cggatgcggc gtgaacgcct tatccagcct
     7861 accaaacccg caccgattgt gggcaacata agcatcgcaa gcgccgcatc aggcatctgc
     7921 gcacaaatgc cttatccgcc taaaaacatt aaacccgcaa taatgcgctt ccgagataat
     7981 cgttcgcgtc cttcactccc ggcagcgcaa aaaaataacc gccgccgata ggtttaacgt
     8041 attcctccag cgcttcgcca ttgagccttt tttgtactgt caggaagcct tttaccagat
     8101 cgtgctggta gcagacaaac agcaacccca tatccagttg tccggagttg gtgacgccca
     8161 gtgaataact gtagccacga cgcagcatca ggctggactc actctccgcc gtgcggggat
     8221 tcgccagccg gatatggctg tccagcgcga tcaccttccc ttccgggtcg ctggcgtaat
     8281 caggcacatc atgctcatgc tgcattccca gcggcgcacc ggtttgctta tcacggccaa
     8341 aaatcgtctg ctgttctttc agcggcgttc tgtcccaaaa ttccactcga aactgaatca
     8401 agcgtactgc ctgatagctg ccaccgattg tccacgcagg ctcctgctga tctgccgtta
     8461 cccacaccac tttttgcatc aacttatcat tctggctatc gggattggca gtgccgtctt
     8521 tgaaacccag caaattaatc ggcgtctctt tgcctttact acgcgccgcg tgatcggaaa
     8581 taaacccttc ccgcttccag cgcacactga gcaaatccgg cgtgtgtttg atgatatcgc
     8641 gcagcgcatg gatcaccgtg tcctgggtgt tggcgcaaat ttgtagcaac acatcaccat
     8701 ggcataacgc cgcatccagc gagtcgttgg ggaaacgcgt catcttctgc aactttttcg
     8761 gcatctgtgg cgcaaggccg aagcgctcat caaacaatga gtgacccacc gataacgtga
     8821 tggtgagatt atcgggcgca atgtagccgc caagaatgcc ggaatcgagt ggtggcaggc
     8881 gcggatttgg cgtttctggt gctgctccgc cctgagtcag aaaagcaaaa cgctgagtca
     8941 acaagcgaaa caaccgctca agatcggctt tatcgctggc aagcacatca aacgccacca
     9001 gcatcattgc ggcctgttgt ggcgtcagga tccctgcctg atgctcacca taaaacggct
     9061 gtttctcatt gcgtgcatcc ggtgaaagcg tgcccggtgc actttgcgtt ttttgtgcat
     9121 gagcgaccgg acaacttccc gccagcgcca gcgcaccaat tcctttcagt aaacgtcggc
     9181 gtgacagttc attcacgccg ttttcatctt catactgcat aacgcttaat ccagtcccag
     9241 cacaccacga agttgcgcca gatcttccgc cagcgcagta atcggccctt tcagcgcgtt
     9301 ccggtcggcc tcggtcaatt tgtcgtaggt ttcaaaaccg tctttagtac ggtatttcgc
     9361 cagaatggta tcgacctttt taaagttggc atcgactttt gccagcagtt ccgggttggc
     9421 tttttgcagt tgtggacgca gcagatcgac aattttctgc gagccttcaa cgttagcctg
     9481 gaaatcccac agatcggtgt ggctgtagcg atcttcttca ccgctgattt tgctggccgc
     9541 cacttcctca atcagtccgg ctgcaccgcc aaccactttt gaaggtggga aagccagttc
     9601 actgatgcgt ttttgtaaat cgaccacatc ggtataaagc tgctcagcgt actgatccat
     9661 tcctttggtg gtgttgtcgc caaacaatgc tttttccaga cggtggaaac cggtgaattt
     9721 tggatcagcg gctttttgct cgtaatcatc ttcacgggcg tcaatgctgc catccagatc
     9781 ggagaacagt tcagcaatcg gctcaatgcg ctcatagtgc tggcgcgtcg gcgcatacag
     9841 tgctttcgct ttttcgatat cgcctgcttt aatcgcgtcg gtaaaggctt tggtgtcggt
     9901 caccagctgt gtggtttccg ccatgacata cgctttatat gcagtaattg caccaccaag
     9961 acttaacagc gcatcacttt gcgccgcatc cgccgttgcc tcacctttga cgatcaactt
    10021 ccctttcggg ttagtcagca gaccgcaggt catatcgtat tcgccaggct gtaaattcgc
    10081 cgtcattttc tggctaaaac caggggcgat attttcccgc tcttccacca ccatcacgcc
    10141 tttgaggatc tcccactcca gcgccttctg gctgtggttc tgaataatga actgtgtttt
    10201 cccggcgtta accgtaatgg tcatcggttc gcactgttta tccgtcacgg tcactttgac
    10261 ttgcggaata tcagcggcgt tagccataaa agcagaagag aacagcgcag ccacgctcaa
    10321 ctgcaatgcg ttacggcgga agttaatggt catgacgaga ctatcccttt aaagtatgtt
    10381 gtaactaagc aagagtttgc gtcgtatttg ctacgcggag cgagacgctg tcgcccctgc
    10441 gcgtggtggc agagcaaatg ccaccagcgc cgggatgaga taaataaacc agacggcgac
    10501 ttcgctgacg ctcggcgctt cctgataacc aaaaatccct tccatcaacg tgccaaacag
    10561 cgagtgagtt gagagcaccg cgctcatatc gaaggcgatt tcctgaaagt ggttccacaa
    10621 tccggcttca tgaaatgcgc gaatggcacc tgcagccagc cctgcggcga cgaagagaat
    10681 aaacaggctg gtccatttaa aaaatgcacc aagattgagg cgaataccgc cccagtagag
    10741 caggaagccg agcaccacgg cagtagcaag accgagcatt gcacccagcg gcggccagat
    10801 cccgacatct tgttgaaatg ccgccagcag gaaaaagacc gactccaggc cttcccttgc
    10861 aacggcaaaa aagaccatca tcaccagcgc ccagccatga tgatttccac gctgcaatgc
    10921 gctatcgact gcctgttcca gttgcacttt gacgttgcgc gacactttgc gcatccagaa
    10981 aaccatccag gtaaggatca ccacggcgat caccgccacg ataccttcaa acagttcctg
    11041 ctctttttgc ggaaattcgc cggtggtttc gttaatgaag atacccaagc ccaggcacaa
    11101 cgcagcggca agcaacacgc caatccacat cacaccaatc catcggcctc gctgggtacg
    11161 cttaagatag ctggcaatca aactgacaat cagcgcggct tcaagccctt cgcgcaacat
    11221 aatgagaaat ggaacaaaca tgccacccac ccttaaacgt ttttctgagt caatctatgc
    11281 aaagaaaagt aaatcgcagt gatagcgatt atcattacgc taaagaaaaa tacaagcgga
    11341 atgtttataa aatgatgttg gaatgtaaaa atgagtagat caaatccgga gaaaaaagcg
    11401 attattttaa ggtatttagc tgacgtttcg cccggcagat gaagcttgta cgaatgttgg
    11461 tggcgacaaa ctcgctgcag cgtgccccga agggttgaat tttcagttag tcgtatcaca
    11521 aaagaacgtt gttttttgct tattaataga tagtgatagg gttgtgaatg aaattatacc
    11581 tcgtctcctc atcgaatttt tcgcccacaa cctaaacagg agaaaacgtg tatttaagtc
    11641 tggaatgagc gatttttgat agtgcgtaat taacccaaaa aattataatc ccccttatta
    11701 tatttccagt gatatcattt tatggctaaa gctatataca actctaaatc acgataaaac
    11761 gaagcaggat gcgcattaaa aaagtcacac ataataaaaa tagaaatctt aaccagaagt
    11821 aatagatcat cgtgaaagtc aatcatcaaa aacaccaaca cacaaaaatc aataagttaa
    11881 taattgttca gggctttttt ctcacgattt ttctactatg acattatcac aaacccacta
    11941 gcatgaatac agaaagagaa gtaataagat gagacgccac tgctttcaga tgaaccctg
//
LOCUS       DAZGBC010000151        11943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGBC010000151 DAZGBC010000000
VERSION     DAZGBC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.152"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..629
                     /gene="yfaZ"
                     /locus_tag="ACHK1J_004000"
     CDS             87..629
                     /gene="yfaZ"
                     /locus_tag="ACHK1J_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO7913207.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQVGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            729..1931
                     /gene="yfaY"
                     /locus_tag="ACHK1J_004001"
     CDS             729..1931
                     /gene="yfaY"
                     /locus_tag="ACHK1J_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO7913208.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2151..2933
                     /gene="yfaX"
                     /locus_tag="ACHK1J_004002"
     CDS             2151..2933
                     /gene="yfaX"
                     /locus_tag="ACHK1J_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO7913209.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2948..4153
                     /gene="rhmD"
                     /locus_tag="ACHK1J_004003"
     CDS             2948..4153
                     /gene="rhmD"
                     /locus_tag="ACHK1J_004003"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO7913210.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4210..5499
                     /gene="yfaV"
                     /locus_tag="ACHK1J_004004"
     CDS             4210..5499
                     /gene="yfaV"
                     /locus_tag="ACHK1J_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7913211.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5517..6320
                     /gene="yfaU"
                     /locus_tag="ACHK1J_004005"
     CDS             5517..6320
                     /gene="yfaU"
                     /locus_tag="ACHK1J_004005"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO7913212.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVDGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6361..6594)
                     /locus_tag="ACHK1J_004006"
                     /pseudo
     CDS             complement(6361..6594)
                     /locus_tag="ACHK1J_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6595..7566)
                     /gene="rpnE"
                     /locus_tag="ACHK1J_004007"
     CDS             complement(6595..7566)
                     /gene="rpnE"
                     /locus_tag="ACHK1J_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO7913213.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSLLQKERLMTIAERLRQEGHQIGWQEGKLEGLQQGKLEGLQ
                     KGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(7759..8949)
                     /gene="glpC"
                     /locus_tag="ACHK1J_004008"
     CDS             complement(7759..8949)
                     /gene="glpC"
                     /locus_tag="ACHK1J_004008"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO7913214.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8946..10205)
                     /gene="glpB"
                     /locus_tag="ACHK1J_004009"
     CDS             complement(8946..10205)
                     /gene="glpB"
                     /locus_tag="ACHK1J_004009"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO7913215.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10195..11823)
                     /gene="glpA"
                     /locus_tag="ACHK1J_004010"
     CDS             complement(10195..11823)
                     /gene="glpA"
                     /locus_tag="ACHK1J_004010"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO7913216.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQSLHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAQTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
BASE COUNT         2665 a         3062 c         3289 g         2927 t
ORIGIN      
        1 tttttcgctc attgaatcct ctgcgccagg cagtacaatc ccgcagcttt gggaattttg
       61 attaattgct tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa
      121 tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggtg ggtaaagaat
      181 acaccaatat tggtgtcggt tttgggactg aatcgacggg cctggcttta agcggtaact
      241 ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc
      301 tcgggccgtt aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg
      361 atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc
      421 gtttgtttgg cgagtattac tactctccgg attcactctc cagcggtatt aaaagttatg
      481 aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt
      541 atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga caacgccgtg gctgacggcc
      601 cgtatgttgg ggttaacgcc agtttctgat cccttccggc gcggcattct gtcgcgccat
      661 tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg
      721 agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta cacgggcaaa
      781 tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg ttgccattat
      841 ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta
      901 gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg gccgaccagt gatgatttaa
      961 gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca
     1021 aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac
     1081 aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt
     1141 ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg tcagaattta
     1201 aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga gcgtttttct ttaccacagc
     1261 cgccggtttg cctgcgtttg actacctttg gtcgttcgga aagcgatctg gcacaaagcc
     1321 tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcctca atgccgatca
     1381 tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa ctgtggctgg
     1441 acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc
     1501 agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag caattcaccg
     1561 gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg
     1621 ttccttcaca ggaggaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgggcca
     1681 accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat ctcaactttg
     1741 cgctagccac gccagacggc actttcgctc tgcgtgtgcg tttcagcact acacgctaca
     1801 gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgctg cgccgttggt
     1861 taaacggcca ggacatcgcc agtgagcatg gctggattga ggttgttgag tccatgacct
     1921 tatccgtctg attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc
     1981 aaaatttgtc caacctgttg tactcaataa gaaaaaatcc gtgcgctgat tcacaaattt
     2041 gtgcgtaatt tctccagtcc gttggacgag gagcgggatt tgatgcgctt cacaatttta
     2101 ctccccctga gcaacgagac tgattccagt atctaagact ggagtccagt atgttggaat
     2161 caagcaaagt tcccgccctg acacgggcta ttgatattct caatttgatt gcccgcattg
     2221 gcccctgtaa tgctgcgacg atcattgaca cattggggat ccctaaaagc acggcctatt
     2281 tgctgcttaa tgaactcagg cgtcagcgtt ttcttagcct tgatcaccag gaaaattttt
     2341 gtttgtggac caggctggtg gagctttccg gtcatgcgtt aagcaagatg gatctccggg
     2401 aactggctcg accgcgtttg acgcaactga tggataccac cgggctgtta tgtcacctgg
     2461 ggattattga taacggcagc gcttattaca ttttgaaagt ggagtcatca gccactatca
     2521 gcgtgcgttc acatgaagga aaaagccttt cgctttatcg ctccggaata ggtaaatgcc
     2581 tgcttgcctg gcaacctgca actgtacagc aaagtattat cgaagggctg gtctgggagc
     2641 aggcgacacc cacaacgatt actcatccac agcaacttca cgaagaactg gcgcgtattc
     2701 gccgtcaggg ctggagctat gacaacggcg aagattacgc cgatgtccgt tgtgtcgctg
     2761 cgccggtgtt taacgccaat aacgagctta ccgctgcgat ctcggtggtc ggtacccgct
     2821 tacaaatcaa tgaagaatac cgtgattatc tggcgggcaa ggccattgcc tgcgccaggg
     2881 atatttcacg tctgttggga tggaaaagtc cctttgactt acaagcctca taacaatgga
     2941 gaacatcatg accctaccaa aaattaaaca ggttcgcgcc tggtttactg gcggtgcgac
     3001 agcagaaaaa ggcgctggcg gcggtgatta tcacgaccag ggggcgaatc actggataga
     3061 cgatcatatt gccaccccga tgagtaaata ccgcgattac gagcagtcac gccagtcatt
     3121 tggcattaac gttcttggca cgttggttgt tgaagtcgaa gcagaaaacg gccagaccgg
     3181 attcgccgtt tcgacagccg gtgaaatggg ctgttttatc gtcgaaaaac accttaaccg
     3241 tttcattgag ggtaaatgtg tcagtgatat caaactgatc catgatcaaa tgctcaatgc
     3301 caccctgtat tactccggct ctggtggcct ggtgatgaac acgatttcct gtgtcgatct
     3361 ggctctgtgg gatctgttcg gcaaagtggt cgggcttccg gtttataaac ttttaggcgg
     3421 cgctgttcgt gatgagattc agttctacgc cacaggtgcg cgtccggatc tggcaaaaga
     3481 gatgggcttt atcggtggca aaatgccgac gcactggggg ccacatgatg gcgatgcggg
     3541 gatccgcaaa gatgccgcta tggtcgcgga tatgcgtgaa aaatgcggtg aggatttctg
     3601 gttaatgctc gactgctgga tgagtcagga cgtgaactat gcgaccaaac tggcccacgc
     3661 ttgcgcgccc tataacctga aatggatcga agagtgcctg ccgccacagc agtatgaagg
     3721 ttatcgcgaa ctgaaacgca acgcgccagt cggaatgatg gtcaccagcg gtgagcacca
     3781 cggcacactg caatcttttc gtacgctttc agaaaccggt atcgacatta tgcagccgga
     3841 tgttggctgg tgcggtggct taaccacgct ggtggaaatt gccgcaatcg ccaaatccag
     3901 ggggcaactg gtggtgccgc acggttcgtc tgtttactcc caccatgcgg tgatcacctt
     3961 cactaatacg ccattcagcg aattcctgat gaccagtccg gattgttcaa cgatgcgtcc
     4021 gcagtttgac ccgattctgc tcaatgagcc ggttccggtg aatggtcgta ttcataaatc
     4081 agtgcttgat aaacccggtt ttggcgtcga actcaatcgt gactgcaatt tgaaacgccc
     4141 ctacagccac taatcactcc gggcgttgct gtgacaacgg taacgctcac atattctaag
     4201 gagcttttta tgagcaccgc tttgcttgac gccgtggtga agaaaaaccg tgtgcgttta
     4261 attccgttta tgttggcgct gtatgtgctg gcgtttctcg accgttcgaa tatcggtttt
     4321 gccaaacaga cctaccagat tgataccggg ttgagtaatg aagcttatgc gctgggagca
     4381 ggcattttct ttgtggtata tgcgtttctg ggtgttccgg cgaatctttt gatgcgcaaa
     4441 ctgggggcca gaacctggat tggtacgaca acactgctgt ggggatttct ttcggcagcc
     4501 atggcatggg ccgatactga agcgaaattt ctgatagttc gcactctgct tggtgctgcg
     4561 gaggccggat ttttccctgg tatgatttat ctcacctcgc aatggtttcc gcagcgtaat
     4621 cgcgccagca ttatggggct gttctatatg ggcgcaccgc tggcgttaac actgggatca
     4681 ccgctttccg gcgcgctgct ggagatgcat ggatttatgg ggcatcccgg ctggttctgg
     4741 atgtttgtga ttgaaggatt gttggcagtc ggcgctgggg tattcacatt cttttggctt
     4801 gatgacacac cggagcaggc acgttttctg agtaaacaag aaaaaacgtt gcttatcaat
     4861 caactggcaa gtgaagaaca acagaaagtg acttctcggc tgagcgatgc gctgcgtaat
     4921 gggcgagtct ggcaactggc gattatctac ctgaccattc aggtggcggt ttacggatta
     4981 attttcttcc tgccgaccca ggttgcggca ttgctgggaa caaaagtggg ctttacagcg
     5041 tcggtggtca ccgccattcc gtgggttgcg gccttgtttg ggacctggct tattccgcgc
     5101 tattccgata aaaccggcga acggcgtaat gtcgcagcgc tgacattact ggcggcgggc
     5161 attggtattg gtctgtccgg gctgctttct ccagtaatgg cgatcgtagc gctgtgtgtt
     5221 gcagccatcg ggtttattgc cgtgcagccg gtgttctgga cgatgccgac acaactgctt
     5281 tccggtacgg cgctggctgc ggggattggt tttgtaaacc tgtttggtgc agtgggcggg
     5341 tttattgccc cgatcctgcg cgtgaaagca gaaacgttat tttccagcga tgcggcggga
     5401 ttactgacgc tggcagcggt ggcggtcatc ggttcgctga ttattttcac tctgcgtgta
     5461 aatcgtactg ttgcgcagac cgacgtggca catcattaaa taggttaagg aacacgatga
     5521 acgcattatt aaccaatccc tttaaagaac gtttacgcaa gggcgaagtg caaattggtc
     5581 tgtggttaag ctcaacaact gcctatatgg cagaaattgc cgccacttcc ggttatgact
     5641 ggttgctgat tgatggggag cacgcgccaa acaccattca ggatctttat caccagctac
     5701 aggcggtagc gccctatgcc agccaacccg tgatccgtcc ggtggatggc agtaagtcgc
     5761 tgattaaaca agtcctggat attggcgcgc aaactctact gatcccgatg gtcgatactg
     5821 ccgatcaggc acgtcaggtg gtgtctgcca cgcgctatcc tccctacggt gaacgtggtg
     5881 ttggggccag tgtggcacgg gctgcgcgct ggggacgcat tgagaattac atggcgcaag
     5941 tcaacgattc gctttgtctg ttagtgcagg tggaaagtaa aacggcactg gataacctgg
     6001 acgagatcct cgacgtcgaa gggattgatg gcgtgtttat tggacctgcg gatctttctg
     6061 cgtcgttggg ctacccggat aacgccgggc acccggaagt gcagcgaatt attgaaacca
     6121 gcattcggcg gatccgtgct gcgggtaaag cggctggttt tctggctgtg gctcctgata
     6181 tggcgcagca atgcctggcg tggggagcga actttgtcgc tgttggcgtt gacacgatgc
     6241 tctacagcga tgccctggat caacgactgg cgatgtttaa atcaggcaaa aatgggccac
     6301 gcataaaagg tagttattga tatcaaaggc ccatggggat cggctgtggg cctgtgttaa
     6361 ttagtggtta ttcgctgcca gatcggcttc gcttagctgg gtggccgcga gcacctggtc
     6421 acgatcaatg ccctgttcca gcatgcgcaa ggcaatttta atagcttgtt catgcatgcc
     6481 ttcctgcaaa ccttctaatt taccttgctg caaacctact aatttacctt cttgccagcc
     6541 aatttgatgt ccttcctgcc gtagccgttc agcaatagtc atcaatctct ccttttaatg
     6601 acagttcgga atatcagcat cagtgagttg ggttgccgcc agcacaatct cacgttcaaa
     6661 gccctgctcc agcatgcgca aggcaatttt aatggcttgc tcatgcatgc cttcctgcaa
     6721 accttctaat ttacctttct gcagaccctc caatttacct tgctgcaaac cttctaattt
     6781 accttcctgc caaccaattt gatgtccttc ctgccgtagc cgttcagcaa tagtcattaa
     6841 tctctccttt tgtagtagtg aacgttcggc aatctcctga ataaaacggg tgaaacggga
     6901 ggtatcgccg cattgcagca gataattaaa cagtgtttgt agctggctgt cattagtgaa
     6961 gcctctaacc aaaagcgtgg tgatcctgtc gaccataccg attaaatcgc ggtcgcgaat
     7021 atgcttttga atcagttcaa gcagagctat acgccgatgt tgcatgatct catcgtcagg
     7081 tacgatggta atatccacca acggaaaagc ttcggtgtac aactgccggg caagttgcgg
     7141 atcgtcaaac tcatccagcc agttgagtga gtacgggtag ggcgaggttt cgccatgata
     7201 aaacagcaac ggcaccacca gcgggactct gtcatagcct ttgtccaggt gacgctgcat
     7261 ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag agctttgatg
     7321 ttcaatcacg cagtagatat agccgtcacc gtcgctggtt tccacggacc acaaaacatc
     7381 cgagtagtaa gcgcgtaaac ttttttcaat aaaactggtg ggttccaggc gtaaggtttg
     7441 caggttgcaa agcttgcgca gcggctctgg tagatgtatt tcgagaaaat cccgtgcggt
     7501 ttcgggtgtg aacataaagg ttttaaatac tgcatcatgc ggggaggagg ttgttgattc
     7561 ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt atcccaggcc
     7621 tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt cactgcgttg
     7681 caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc acatgcttac
     7741 cgggcatcag aaaggagttt aagccagcgc ctgggccagt agcgtaatcg gatgttcgca
     7801 gcgaagactt gtggacatct caatctgcca tttacaggtt tcgcagtcgg tgaccaccag
     7861 atctgcgccg ctttcttcta tctggcggaa cagtggtgcg ccgatggctt gtgaggtggg
     7921 gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg aatccagcac
     7981 cgttaactca agccccggga tgttacgcaa cagctccagg gtgtagagcg tccagcccat
     8041 tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt tcagcggtaa
     8101 cgttttgcct tcgtccagct tgcgccacag ccagcgggtc gccagttcga tatgatcgcg
     8161 caagcctttg ttgtcgacat tcagcacttc cgggtactcg tcgcgcaggg cgaaagtaca
     8221 ggtggaggag gtggcaatca ccggaatgcc ttttactccc acggcttcgc ggatcgactc
     8281 tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga ttagcggcac
     8341 accgcagcat ttttctttgc tgagcagttg cacgccagta cccatcgcgt tgagcacttt
     8401 aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt ggaaaaaggc
     8461 gacctggtct ttatactgcg cctgctgggc cgccacgctg cgataccagc gacgaaacgt
     8521 gccgaaggag tatttcggta gcgtgcggcg atgatcgatt tttaacgccg catcaagcaa
     8581 ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg gcgtggaaac
     8641 gctacccatc aggtcggtat ggcttaaaac aaaattgcgc agcgacgggc gcgtggtgtc
     8701 atatttcgcc cgtgcgcgct ggatgatatc gccaatcttc acatcggacg ggcaggcgac
     8761 ttcgcaacgt ttgcagttga tgcaatattt cagtgcctcg tcatacagtg cgccatcttt
     8821 taaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac cgggattcac
     8881 ccggctcact gggcaggcgg tggtgcagac ggtgcactta atgcagtttt cgaagctggt
     8941 gtcattcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg taaagcactg
     9001 acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc cagcaccgaa
     9061 ccgatggcaa acaggttttc gatggtttgc cctgcctgcg acgggcgtag cgtctgatca
     9121 gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatctccctt ataccattca
     9181 ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctctcgaat gccgttacgt
     9241 tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa acgtggacgc
     9301 agcgggatat cggcgtgatt gcgggtccag atttcattca ctacgccatt tttacaggtc
     9361 acttttttca cttcatcgcc cggcatccac acgccaccct gacgcacaaa ctggcgctgt
     9421 aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa aagcatcagt
     9481 gaacaaggta gtttttcatt caaccaacgc cacagtttgt catcagccag gccgaagcag
     9541 gcgggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc aagtaacagc
     9601 ggccagtttt cttcattatc aaggaaacgg gcgatattca ccgcgcggaa ctcggtggcg
     9661 ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac ggcaaggccg
     9721 agttcacgca acgaagctgc cgcaaggtgc gcctgaaaat ccatcagtcc actaattccc
     9781 actacacata ttttcttcgc gggcagcggc cagacgggga cttctggcga acttagccag
     9841 gtagagcgca gagtgccgag cggcgtaatc cgctggtgag ccagttctac gctgccctgc
     9901 aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc gagcacgcgt
     9961 tgtggcccga gaagagagta aggatgtgca ggtgcctgct gacgcaaaga ttccagtcca
    10021 ctgtgaatgt ctgtcaccgg ttgaccatct ggcagatggc tcagcaaatc cagcgaaccg
    10081 gatgagaaat gcagcgcgct ttgaccacgc gtgacaatgg cacagcgcag gccgtgtttt
    10141 tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac agtatcaaag
    10201 cgcatctttc tgctccttct ccagaccaca caatccctga taaacccagc gggtaaattc
    10261 gctttcgcgc agtgcatctc cccaggcgat gggttgcacg cctttccagc gttcgttaag
    10321 gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaaac gttgcagcag
    10381 tccggcagcg cggcaggcgc agagttcgcc ctggcaggta cccatcccca cacgggtacg
    10441 acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact gcacttcacc
    10501 cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt cgctcagcca
    10561 ggcaggcgta cgatcgccat gacgataaac cgcagaaccg cgcagcgggg caggcaggga
    10621 gatgacttta cgcaaggtaa cttcagcggg ttcctgtgaa cccggaagtg ccagatcggc
    10681 ggtcgtacag gggcgcgtgt tgcccagttt gcggcatacg gcgtcggtag cccattccgc
    10741 catcagccga taggtcatca gtttgccacc ggtaatggtg ataaatccgt ccagaccatc
    10801 gcgttcagca tgatcgagca gcacgatgcc gcggctgacg ttacgcccgc tcggatcatc
    10861 atcgctggcg accagtggac gcacgccaga gtaggcccgc aaaatgcgcg tttgcgccat
    10921 cacgggggcc agtttttccc cttcacgcag caggatatcc acttcttctg ccgtcacccg
    10981 attatcgtca atctcgttgt aatcaatacg taaagaggtg gtgccaatca gcgaaatggt
    11041 atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga tcacatgctg
    11101 gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc gaatgcgcag
    11161 atcggcatat tcggcaatgt gttgccccca gatcccggca gcgttaacca cgacaggcgc
    11221 atgtagcgac tgggtttcgc cggtgaggtg gttgcgtaca tgaacgccgc acaccgttgc
    11281 gccttcacga atcagtcccg tgacttcatg agcagtaagg ataacggcac cgtgttcttt
    11341 ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat ccggaacttt
    11401 caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt gctgcgggtc
    11461 tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg cctggaagga
    11521 gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc agtgacgtgc
    11581 aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg catcggttac
    11641 cgcgtagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac cggttgcgat
    11701 gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc gggcaatccc
    11761 ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt cgcgagtttt
    11821 cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa catgaattgt
    11881 ttgatttcgc gcatattcgc tcataattcg aaagtgaaac gtgatttcat gcgtcatttt
    11941 gaa
//
LOCUS       DAZGBC010000152        11910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGBC010000152 DAZGBC010000000
VERSION     DAZGBC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.153"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..417
                     /locus_tag="ACHK1J_004011"
     CDS             <1..417
                     /locus_tag="ACHK1J_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7913217.1"
                     /translation="TAYRDGAGIWTICRGATQVDGKPVVPGMKLSKEKCDQVNAIERD
                     KVLAWVAKNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRW
                     WIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            708..839
                     /locus_tag="ACHK1J_004012"
     CDS             708..839
                     /locus_tag="ACHK1J_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913218.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            803..957
                     /locus_tag="ACHK1J_004013"
                     /pseudo
     CDS             803..957
                     /locus_tag="ACHK1J_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1022..1483
                     /locus_tag="ACHK1J_004014"
     CDS             1022..1483
                     /locus_tag="ACHK1J_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7913219.1"
                     /translation="MNRILYVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREAPTTSGVDNATAPRLADTVERDYFTLRERLMLMQKQLEGAQDYIRTQC"
     gene            1783..2589
                     /locus_tag="ACHK1J_004015"
     CDS             1783..2589
                     /locus_tag="ACHK1J_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO7913220.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            2570..4276
                     /locus_tag="ACHK1J_004016"
     CDS             2570..4276
                     /locus_tag="ACHK1J_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO7913221.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGACGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            4276..6420
                     /locus_tag="ACHK1J_004017"
     CDS             4276..6420
                     /locus_tag="ACHK1J_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO7913222.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            6578..7585
                     /locus_tag="ACHK1J_004018"
     CDS             6578..7585
                     /locus_tag="ACHK1J_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913223.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEYLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            7609..8823
                     /locus_tag="ACHK1J_004019"
     CDS             7609..8823
                     /locus_tag="ACHK1J_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO7913224.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            8879..9268
                     /locus_tag="ACHK1J_004020"
     CDS             8879..9268
                     /locus_tag="ACHK1J_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913225.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            9318..9779
                     /locus_tag="ACHK1J_004021"
     CDS             9318..9779
                     /locus_tag="ACHK1J_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913226.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            9763..10326
                     /locus_tag="ACHK1J_004022"
     CDS             9763..10326
                     /locus_tag="ACHK1J_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913227.1"
                     /translation="MAELSDFLPYVRRQISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDADISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            10326..10976
                     /locus_tag="ACHK1J_004023"
     CDS             10326..10976
                     /locus_tag="ACHK1J_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO7913228.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLSCVPGARQTLPDGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRG
                     KPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            10973..>11910
                     /locus_tag="ACHK1J_004024"
     CDS             10973..>11910
                     /locus_tag="ACHK1J_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO7913229.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKG"
BASE COUNT         3045 a         2764 c         3425 g         2676 t
ORIGIN      
        1 acggcatacc gtgatggtgc gggtatctgg accatctgcc gtggtgccac ccaggtggat
       61 ggtaagcctg tcgtccccgg catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc
      121 attgaacgtg ataaggtgct ggcatgggtg gcgaaaaaca tcagagtgcc actgaccgaa
      181 cctcagaaag cggggatcgc gtcattctgt ccttacaaca ttggccccgg taagtgtttc
      241 ccgtcgacgt tttataaacg aattaatgca ggcgatcgca ggggagcgtg tgaagcgatt
      301 cgctggtgga ttaaggacgg tggcagagac tgccgtatcc gctcaaataa ttgctacggt
      361 caggtatccc gtcgcgacca ggagagtgcg ctggcatgtt ggggtatcga caggtagcag
      421 aatattttgc tgaaaaataa cgttggtcaa cgcggacgga taacacgaaa tcctgcgaac
      481 tggcaaaacc taagtgaata aaagtaaaaa ccccgttttt ggcagcaagc ggggttttgt
      541 gtttatggca gtaagctatg ggtggctgcc ttgattgatt ttagcaaact gattagggag
      601 ttgcgactca tgattagtca attaccaaac tggaagtttt tgctggtctg gagcatccct
      661 tttttatggg gggtatccca gttaattgtg gcaattaagg ggtagctatg tcagacaaac
      721 tcataacgct ggcgaagatc ctctgtgtaa tcgtcggcat ttcattttca ctaatgctgg
      781 ttgctatttt tcttttcctg ccctggatga tgttgtcttc gtcggggatg ctggggtaac
      841 agtgactgat gacatcagca gagcgctggc tttgctatta agtgggtggc ggttggtatc
      901 gctgtgtctc cgatactgta tgggctggca aaactggtca ttgcgctgaa atcgtgaact
      961 ttatttaaac agatgagtaa tgaaattgct gtactggtcc gggcaattcg ctggtggtga
     1021 tatgaaccgt attctgtatg tggtgattat tgtactgctg gtggcctgtg gtgcgcttag
     1081 tctggggctg aatcattacc gcgataacgc catcacctac aaagcgcagc gcgataaaaa
     1141 agccagagag ctggagctag caaacgcaac cattactgac atgcagcagc gccagcgtga
     1201 tgttgctgcg cttgatgcca gatactcaag ggaattagcc gatgcgagag ctgaaaatga
     1261 aactctgcgc gctgatgttg ccgctggtcg taagcgcctg cggatcaacg ccacctgctc
     1321 cggtaccgtg cgtgaagccc ccaccacctc cggcgtggat aatgcaaccg ccccccgact
     1381 ggcagacaca gttgaacggg attatttcac tctcagagag cggttgatgc tgatgcagaa
     1441 gcagctggaa ggggcacagg actatatccg cactcagtgc tgaactaagt tttgctgatg
     1501 cgccgtatcg tcgccgtatt cccgcataaa cagagaccgc agccacctta tctgcgtgag
     1561 tgtgcggggg taatcaaaaa cgatgcacac cgggttttct catttttcac gagatgggag
     1621 cgatttcccg cgaagccgcc tgtccggtgc ggtggtggaa gaaaccggat aaaacaacct
     1681 cattgtacaa atatcgatca agtatggcgc tgctgtgtga attctgaaaa atcacagcgg
     1741 tcattatgcg tcagttttta acagaggacg tcagaaagtg acatggcaaa gctggactgg
     1801 aaaaagctgg agcaggcatt ccgacgcgaa catgccgaaa cgggaataac attactggac
     1861 tggtgccgga agaaaaagat taattacaac accgccagaa cccgtataaa aatgggcaaa
     1921 atcgatcatg aaattgatca taaaaccgat catgaaatcg atcatgacat ctcagatgaa
     1981 gaacccagca atgacgcgtg ttccgacgat gaaaaatgcg caaaaaactc tgaaaaaaac
     2041 tgcgcaaatt cggcagaaac gaaacggatt cgtggttccc gacttttacc tccttcaaac
     2101 gctttttctc agcgaaacac ccacgccgta agacaccgtg gatatgcgaa gtatcttgag
     2161 gcagataacc tcatggatga tgcgtccgac atggtgctgt tcgatgaact ggtgttcacc
     2221 cgggcccgcg cactttcagt aactaaggca cttaaaggga tgttcgccga cctggaagag
     2281 gcaactgacg tggaaacccg cgttgctctt tacgacaaaa tactcaaagc tgaacaggcc
     2341 cttgaccgga atattgcccg tatcgagtca attgaacgct cattgctgac gctggacgtc
     2401 ctggctgaga cagcaccaaa acttcgtgct gaccgggaaa gaatcaacgc cgccagagat
     2461 aaactcagag cggaaaccga tattctgacc agccagcgcc ggggtgttat tacgccagtc
     2521 agtgacatcg tgtcatcgct gcatgaaatg agtaattcgg ggagactgga tgacattccg
     2581 gaagaataaa acgcgatgtg atgaacctgc agaaatgact gagaccgaac aacgtctgtt
     2641 cattatgaca aaactgagca atccctggtg gcgcctcaat catctctaca aaatacagaa
     2701 cgaaaaaggt gaactggtca ccttcagaat gcgaccggcg caacgtcagt tgtttcgtaa
     2761 catgcataat aaaaacatta tcctgaaagc gcgccagctg ggattttcca cagccattga
     2821 tatttatctt ctcgaccagg cattattcat tccgcatctc aaatgcggga tcgtcgctca
     2881 ggataaacag gctgccagtg aaattttccg cacaaaaatt gctgtaccgt ttgatcatct
     2941 ccctgactgg ctgagagcct cattcaccat cgttgaacgt cgtagcggtg cctgcggtgg
     3001 ctatatcctg tttggtcacg gctcgagtat tcaggtggca acctcatttc gctcaggtac
     3061 ggtgcagcgc ctgcatatct cagagcacgg caaaatttgc gcgaaatatc cggctaaggc
     3121 gaaagagctg cgaaccggta cgcttaatgc cgtctctgat gaatgcatta tttttgatga
     3181 atccacggct gaaggcgtgg gtggtgattt ttacgagatg agtaaccgtg cacaggagag
     3241 cactgcatca ggcttattgc tgacggcaca ggattataaa ttccattttt acgcatggtg
     3301 gcaggatcct aaatacagcg ccagagtgcc tgaaagcggg ctgaagctgt cacgggaaaa
     3361 aatgacgtat ttttctgcgg ttgagaaggc aatgaacatc acgcttaccg atgagcagaa
     3421 gcagtggtac atcagtaagg aaactgaaca gcgtgaggaa atgaagcagg agtttccctc
     3481 aacgccacag gaggcgtttc tgacgtccgg acgacgtgtg ttcagtgccg aaagtacatt
     3541 gcaggcagaa tcattctgtt cgccaccgat gattgtttat gacattgaac ctgttacagg
     3601 agcgaagact aaagctcagt ctctgcgtga aggaaataaa aacgagttgc agcggacgct
     3661 gatgaattat ctgctggtat gggaactgcc ggatccggat gaagagtatg tttgtggggc
     3721 agatactgcc gaagggctgg agcacggaga ccgctcatcg ctggatgttg tcaaacgcag
     3781 taatggcgag caggtggctc actggttcgg gcatctcgat gctgaacttt ttgctcatct
     3841 catttcgcag gtctgtcgta tgtataacaa cgcgtttgtg gggccggagc gtaataatca
     3901 cggacatgca gttatcctga aactccggga actctatccg acacgttata tctacaacga
     3961 acagcatctt gaccaggcat atgacgacga tacgccccgc cttggctggc tgacaacccg
     4021 tcagagcaaa cctgttctga ccgaaggaat gaaaacgctc ctgaataatg gaatatcagg
     4081 gatccgctgg tcaggcacat tatcggaaat gaacacctac gtttatgacg cgaaaggctc
     4141 catgaatgca caggaaggct gctttgatga tcagctcatg agctacatga ttgcccagga
     4201 gatgcgcgcc agaatgccgg tgagggtaaa acagaaaacg gataaacgca gaaccacaca
     4261 ctggatggca cactgatgaa aaatgaaatt aacaccacag cgatgaaaaa cgatcatgga
     4321 tccacgccgc gtttttctca gcgtcagtta ctgtctctct gttctgatat tgacagtcag
     4381 cctctctggc gtgatgccgc aaacaaggcc tgtgcgtatt atgatggcga ccagctggca
     4441 ccggaagtta tccaggtact gaaagatcgc ggtcagccca tgaccatcca taacctcatt
     4501 gcccccacgg tagatggtgt actgggaatg gaggcaaaaa caagaacgga cctgatagtg
     4561 atgtcagacg atccgaacga tgaaacagag aaactggcag aggccattaa tgcggagttt
     4621 gctgatgcgt gccgtcttgg caacatgaat aaggcccgct ccgatgccta tgcggaacaa
     4681 atcaaggcgg ggctcagttg ggtggaggtc aggcgcaaca gcgatccgtt cggacctgaa
     4741 ttcagggtat ccacagtcag cagaaatgag gttttctggg actggctcag ccgggaatcc
     4801 gacctgagtg actgccggtg gctgatgcgt cgccgctgga tggataccga tgaggcaaaa
     4861 gccacgttcc cgggaatggc tcaggttatc gattatgcta ttgatgactg gcgtggtttt
     4921 gtcgatacca cgattactga aggccagccc agtccgttga tgagtgcatg ggaagagtat
     4981 cagtcatggg atcgcaggga gaatgaatgg cttcagcgtg aacgccgtcg tgtgctgctt
     5041 caggtggttt attaccgtac attcgagcgt cttccggtga ttgaactcag taatggacgg
     5101 gtggtggctt ttgataaaaa taatctgatg caggcggtgg ctgtggcatc cgggcgggtt
     5161 caggtgaaag tcgggcgggt aagtcgtatt cgtgaagcct ggtttgtggg cccacacttt
     5221 attgtggatc gcccctgtag tgctccgcag gggatgtttc cgctggttcc tttctgggga
     5281 taccgaaagg ataaaaccgg ggagccatac gggttaattt cccgcgccat tccggcacag
     5341 gatgaggtga attttcgtcg tatcaagctg acctggctgc ttcaggccaa acgcgtgatt
     5401 atggatgagg atgccaccca gttgtcagac agcgacctga tggagcagat cgaacgtcca
     5461 gatggcatta ttaaactgaa tccggcccga aaaaatcaga aaagtgtcgc agatgttttt
     5521 cgggttgagc aggattttca ggttgccagc cagcagtttc aggtcatgca ggaatcggaa
     5581 aaacttatcc aggataccat gggagtgtat tccgcatttc tcgggcagga ttcaggtgcg
     5641 acgtcaggcg tggctatcag taacctggtg gagcaggggg ccacaaccct tgcggaaatc
     5701 aacgataact accagtttgc ctgccagcag gtgggaagac tgttgctggc ttatcttctc
     5761 gatgacctga aaaaacgccg taatcatgca gtggtgatta atcgcgatga tcgccagcgt
     5821 cgccagacca ttgtcctcaa tgctgaaggt gataatggtg aactgaccaa tgatatttca
     5881 aggttaaata cacatattgc gctggcgcct gttcagcaga caccggcgtt taaggcacag
     5941 cttgcacaga gaatgtcaga ggttattcag gggctgccgc ctcaggtgca ggctgttgtg
     6001 ctcgacctgt gggttaatct tctggatgtg ccgcagaaac aggagtttgt tgagcgtatt
     6061 cgtgctgcgc tggggacgcc aaaatcaccg gatgaaatga cgccggaaga acaggaagta
     6121 gcggcacaac aacaggcact tcagcaacaa caggcagaac tccagatgcg cgagatggct
     6181 ggcagagtgg caaaactgga agctgacgcc gccagggcac atgcagctgc acaacgggat
     6241 aatgccagtg cacagcggga agtcgccctg acacaggggc agcgttatgt ggatgcgctt
     6301 aaccaggcac atacggcaga aatcattacc ggcgtacaga atatggaaca ggagcaggac
     6361 gttcttcagc aacagatgct gtatacgtta caacagcgga tgaatgaaat gtcgctctga
     6421 aaactctggc ttcaactgaa ccccgtcatc gtacggggtt ttttgtttcc ggaggtaagc
     6481 gttccgggag cggtgcgctt attcgcgggg gcagcgataa gccttattta ctcaaccatt
     6541 cggatctgtc cgataaacag accatgcgga gttatttatg gattttgaat ttacgggtga
     6601 agaaaccccg gaacaactgg aaaaaatgct ggaaggactt ggggatgtgg atattgacag
     6661 tcacgcacag gacgtcgtga cggaagatac cacggaaaaa catgcggatg aggaagcaca
     6721 gactcagacg ggcgataaca atgtggcacc gacgccggat gccagtgtgg agcagacgca
     6781 ggacgtgaag gagccggaag cgaagggggt gctcacccgc gacggtaaac acgtcattcc
     6841 ctatgaagtc cttgaggctg aacgttccgg taagcaacgg gccgaacagg aagccgcact
     6901 tcttcgtggg cagatagctg aagaaaaacg cagggtggaa ctgctgacat ctcagatcca
     6961 ccaggccggt atgaagccca caccgttacc ggaaaacgaa aaaatttctg atgagcagat
     7021 tgcccgtatc agggagatgt atccggaaat tggtgacgcg gtggcttcgc tcatccgtaa
     7081 aaataactat ctccagtccc gtgttcagca atcaacacag caggcagaag gtaatggtgg
     7141 tgagtattta tcaccggttc ttgatgcgat gaatgccgtg ccggtgctga aaacgtggca
     7201 ggagtccgat ccggatcgct tctcggttgc tgtatccatc gacgggaagc tccagaatga
     7261 ccccgcatgg aaagacaaaa cgctcactga acgtttcgct gaagtggccc gtcgtacgca
     7321 ggttgctttc ggtgaagtca gtgagtcgtc tgctgacaac caggcagaca aaacggatat
     7381 ccggaaaacg gcggaagaaa aagtgaagac cgctgaacag gagcaggcag tacctgcttc
     7441 cccgtcagat ttaggcacca cggcctccgt cggaaccggt gataattttg aacggttact
     7501 tggtgcttct cattcagagg ctgaggcgat tatgcgtggt atgacgaatg ctgaaataga
     7561 cgcacttctg gagaagctcg ggtaacttac tgaaggagta ctgaagtaat gacgactgta
     7621 acatcagccc aggcgaataa gctgtatcag gtggcgcttt ttaccgctgc caaccgcaac
     7681 cgctcgatgg ttaatatcct tactgaacag caggaagcgc caaaagcggt ttcgccggac
     7741 aagaaaagca cgaagcagac cagcgcaggt gcgccggttg tccgtatcac agaccttaac
     7801 aaacaggccg gtgatgaagt gaccttcagc atcatgcaca aactctcaaa acgtccgacg
     7861 atgggagatg agcgtgttga aggtcgtggt gaggatctca gccatgctga cttctccctg
     7921 aaaatcaatc agggacgtca cctggtggat gcaggcggac gtatgagtca gcagcgcacg
     7981 aagtttaacc tggcatcctc tgccagaacg cttctgggga cgtactttaa tgacctgcag
     8041 gaccagtgtg cgatagtgca tcttgcggga gctcgtggtg attttgttgc tgacgacacc
     8101 attctgccga cagcggagca ccctgaattc aaaaaaatca tgatcaacga tgtactgcct
     8161 ccgacacatg accgtcactt ttttggcggt gatgcgacaa gctttgagca gattgaagcg
     8221 gcagatattt tttctattgg cctggtggac aatctctccc tgttcattga cgaaatggcg
     8281 catccgttac agccggttcg tctgtccggt gatgaacttc acggagaaga tccatattac
     8341 gtcctgtacg tcacgccgcg tcagtggaat gactggtaca cctcgacgtc cggtaaggac
     8401 tggaaccaga tgatggttcg tgccgtgaac cgtgcaaaag gttttaatca tccgctgttc
     8461 aaaggtgaat gtgcgatgtg gcgcaatatc ctggttcgta agtatgcggg tatgccgatc
     8521 cgtttctatc aggggtcaaa ggttctggta tcagagaata acctgacggc aaccacgaaa
     8581 gaggtcgctg ctgcaaccaa tattgaccgc gccatgttac tgggggctca ggcgctggca
     8641 aatgcttacg gtcagaaggc gggcggtcac ttcaacatgg ttgagaagaa aacggatatg
     8701 gataaccgta ctgagatagc aatcagctgg atcaacggtc tgaaaaaaat ccgtttcccc
     8761 gagaagagcg gcaagatgca ggatcacggc gtgattgccg ttgatacagc agtgaagctc
     8821 tgattttttc ctttccccat gccgggtttt cgcccggctt tttcaggagt cattaattat
     8881 ggcaaagact atccttgccc cgtcactgag tgaacgggtc tatacgggta cgcacggtaa
     8941 tgagtcggtg gcagaaggcg tatttacggt gaatgctgcg gaagcggaca gtgttattca
     9001 tcttctctca ctgccagtgg gcatccgtat caactcactc cagctggttt caacgggtgg
     9061 tctgggtact gcaaccgtca gcattaagtc cggtgagcat gctctcatcg ataacagcga
     9121 agctgtttct gcaaaatttg ccagatatgt gccagtggag ccgtacacca cacagcgtga
     9181 cggggagctg gttactgtca ccattaagac tgccgctgca accggcaccc tgaatgttct
     9241 gctgcgttat accgtggtgg gatactgatt aaaaccttcc ggcccgcgtc atgcgggctt
     9301 tttatccggg gaattatatg agtgagaaaa ttgccgttgt ctatatcggc ccaaaacccg
     9361 tgaaaaagga caccattacc ggaagccgca cattgttccc gcgtcttgag ccggtgcatg
     9421 ttgacagtgc gatggcctgg caactgctgg ggtttccgga tgtctgggtt cgtcatgaag
     9481 agcttgatga tgttctgaaa aagcaacaac agaatgagca gttgcggcag gcacagcagg
     9541 cgcaggaaag agtgcttgct gcgcgggcag aagcggagaa cagttttgtt gtttctgtta
     9601 acgggcagga ggtggattta agtaagctca cctcagcacg gctggcgacg ctgtgtgagg
     9661 cagaagagct ggatattcac aaagacccga aagaaacggc tgaggcattc cgtatccggg
     9721 tgcgtgaggc atttcgccgt cgtgttgcgg agactgaaca gcatggcgga actgagtgat
     9781 tttttaccgt atgtccgtcg tcagatcagc ggtccactga acattatgat gacggatgct
     9841 ctgtcaatgg ctgccgtggc attcagccgt cagtcgctgg tgtgccgtcg ggaggttact
     9901 gttgtaccag tagcaggaaa agaaatcgtg cttccgtatg acaaagatga tgaggagtgc
     9961 gttcatatca tccgtatctc tgacgataat catgagcttt ttgtcggtcg ggatgcggat
    10021 atcagctccg gacgctccct gcgatttgcc tgttctcccg gtgaggtgag cgtgctttat
    10081 gccgtcgctc cgaaagccgg acgcagccag ataccggatg aactcctcac atggcctgaa
    10141 gaagtggcag cgggggcact tgagcggttg ttcatgcaga ctggtgtttc atggtcagac
    10201 ccgttacgcg cacagtattt ttctgtgcag ttttctgagg ggatccgtcg ggcatatcgt
    10261 catacactgg cgacaagtcc gtactcctca taccgcaatc ctgtccgcag acaaaggttt
    10321 tactgatgac gacgattact gaaatcatcg gacgagtgaa cacgcaactg gttgacccga
    10381 tgatggtgcg ctggcctctg gctgagctgt gtgattatta caatgatgct gtgcgggcgg
    10441 tgattctggc aaggccggat gcaggcgcaa gcctggaaac gctcagttgt gtccctggcg
    10501 cacgccagac tttacctgat ggcgcaatac agcttcttga cgtgatatgc ctcagcgatg
    10561 gcagtgcgat taaaccagta tcccgggagg tgcttgatgc acagtatccc gactggcaca
    10621 tgctgagggg aaaaccggaa tgctttatca gcaacgacct gtccccgcgc gtattctggc
    10681 tgtttcctgt ccctgacaaa gagataagta ttgatgcagt ggtaagccgg ataccggagg
    10741 cagtgtatgt tctgacgcag gacgatgata cgccagttcc actggaagag gcttatgtta
    10801 acccactggt ggactggatg ttgtttcgtg ctttcagtaa ggatgctgcc ggtggtgcag
    10861 aatcggggct ggctgcgcag cattatcaga gttttgttga gcaacttggg atcaaacagg
    10921 gggcagacag tgcattgtct gcccgtaaaa aagtgtttaa cggaggtgga gtgtgagtgt
    10981 tgttgtttcg gggacgctga aatctcctga tggtgaggcg atatcaggag caaatattac
    11041 cctgacggcg ctgacagttt caccggatgc gctcagcggc accagtgcgt cggcagtgac
    11101 ccgtgaaggt ggatattacg gaatgacgat ggatccgggg gagtatgcgg tttcggtgac
    11161 ggtgaaaggg aagactgctg tctacggacg tgtgcgtatt gaggggaccg aaagtacggt
    11221 gacgctcaat atgctgttac gccgcagtct tgttgaggtg agcatacccg gagagctgct
    11281 gacagatttc cggcagatac agaataatgt ggctgatgac cttgccacta ttcgtcgcct
    11341 gaatgaagac acggcgacaa aaaacactca ggccacacag tcaaaagaaa gtgcagcagc
    11401 cagtgcgaag agtgcatctg acagtgcaaa gacggcaacc agcagggcgg ctgaagccgg
    11461 acaaaaagcg actgatgcca ctgaggccgc gacccgtgca atcacagcag cggggaatgc
    11521 agaggaaagc tcgacccgtg ccggagagtc tgaaaaagcc gccggagctg atgcagaaaa
    11581 agccagacag catgctgaaa aggccaggct ggcgcaggag agcgccggag agatccttaa
    11641 gcgggcagag gctgccactg tcagtgctga agaggccaga cgtatggctg agaatgcacg
    11701 ggggccccgg gggcctcagg gagaaactgg tccgaagggg gatgtcggtc ctaaaggcga
    11761 aacaggtcca gtgggccctc aagggcccgc agggccgaaa ggtgagcgtg gtgacgttgg
    11821 tgctcagggg gctgtagggc ccgctggtcc gcgtggtgag aagggcgaac agggggagcg
    11881 aggaccgcag ggaataccag gcctgaaggg
//
LOCUS       DAZGBC010000153        11891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGBC010000153 DAZGBC010000000
VERSION     DAZGBC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.154"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..77
                     /locus_tag="ACHK1J_004025"
     CDS             <1..77
                     /locus_tag="ACHK1J_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO7913230.1"
                     /translation="DFTYGYDEDIKEFIKDKTYPYGNW"
     gene            109..375
                     /locus_tag="ACHK1J_004026"
     CDS             109..375
                     /locus_tag="ACHK1J_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7913231.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            379..1527
                     /locus_tag="ACHK1J_004027"
     CDS             379..1527
                     /locus_tag="ACHK1J_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO7913232.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGTLLFIIRASGY
                     VKVLNDFSIWWLALTPSGCWFFLFCLRHWQWSNQMDEHLFLKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            1520..4909
                     /locus_tag="ACHK1J_004028"
     CDS             1520..4909
                     /locus_tag="ACHK1J_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO7913233.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDI
                     RHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPVDGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTDDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPAIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVTVPESRLHGPLATTFGPVLALMDNQNNSADLLNLQTYLTRITQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLAAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNTRWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLRREGNRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPEMVFELSGTSAFTGNDEDAGDSVEETD"
     gene            4909..6507
                     /gene="tssA"
                     /locus_tag="ACHK1J_004029"
     CDS             4909..6507
                     /gene="tssA"
                     /locus_tag="ACHK1J_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7913234.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADEVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLAELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNPGSS"
     gene            6509..7201
                     /locus_tag="ACHK1J_004030"
     CDS             6509..7201
                     /locus_tag="ACHK1J_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO7913235.1"
                     /translation="MSVMHYLQDKTFQDIVKNLVLPLLSGIIIPLVKWFVQHYGYAPN
                     IRKYRFEKIPVSEKESILARIDKLTKEPTTKNTLVRIKYCYEQMGIYLPIWCCNKLIC
                     FISDRNVSSVDNRLHCFLKYSFVGIFSDGKFTVNTRRVHKGYRMIAVFAVFSLCVQFT
                     GGIFTTMPFLSGGNTVLFMLFSLVYFIMIFLTVIFTCNLINEIRLAVQFGRLFEAWLK
                     SERESPEQLALF"
     gene            7209..8972
                     /gene="tssF"
                     /locus_tag="ACHK1J_004031"
     CDS             7209..8972
                     /gene="tssF"
                     /locus_tag="ACHK1J_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7913236.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KIAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVENVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENVSLPAGISGFDLE
                     VVLSQPWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVTGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEVDRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVSVRNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            8927..10027
                     /gene="tssG"
                     /locus_tag="ACHK1J_004032"
     CDS             8927..10027
                     /gene="tssG"
                     /locus_tag="ACHK1J_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7913237.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYPGSRLDVRLQLCVKRSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTISLGRYQRVQEHYQRKEAQENGDYRW"
     gene            10008..10544
                     /gene="tssJ"
                     /locus_tag="ACHK1J_004033"
     CDS             10008..10544
                     /gene="tssJ"
                     /locus_tag="ACHK1J_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7913238.1"
                     /translation="MAIIAGKTGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWLQPGGSVAVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            10547..10996
                     /gene="tssE"
                     /locus_tag="ACHK1J_004034"
     CDS             10547..10996
                     /gene="tssE"
                     /locus_tag="ACHK1J_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7913239.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSEEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTSILQGMPGTAHQLMCVLSDVLLKYEPRIKRVDVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            11049..11354
                     /locus_tag="ACHK1J_004035"
     CDS             11049..11354
                     /locus_tag="ACHK1J_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913240.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            11409..>11891
                     /locus_tag="ACHK1J_004036"
     CDS             11409..>11891
                     /locus_tag="ACHK1J_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFO7913241.1"
                     /translation="MTSEVKLKTGNDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILARHELKQSSKVALPE
                     A"
BASE COUNT         2724 a         2773 c         3369 g         3025 t
ORIGIN      
        1 gtgatttcac atatggatat gatgaagata ttaaggaatt tatcaaagat aaaacttatc
       61 cttacggtaa ctggtgactc aaagtgaaaa tttatttttg agagttttat gtctaaaggt
      121 tttgtattgc ttggtgataa taccacacat ggtggtaaag ttatttcggc atcttcaacc
      181 atgattgtcg atggtaaaaa agtagctctt gttggagata tggttagttg ccctgtttcc
      241 ggacatggta ccaatcctat tgtggagggc tctcctgaat ggatatctga tggaagagct
      301 gttgtgattg atggatgtaa atgtctgtgt gggtgtaagg ttatatccag tgcaccggaa
      361 tgtgcaatag gataaaccat gggatggagc agaacaaaat ctttatcaac agaattacct
      421 cataaaccat caatgatgtt gtggtttatg acaggggcat ttatggctgt tgttggtact
      481 ctgttgttta ttatccgggc atcaggatat gtgaaagtat taaatgattt cagtatctgg
      541 tggcttgcat tgactccttc ggggtgctgg tttttcctgt tttgcctgcg acactggcag
      601 tggagcaacc agatggatga acatttgttt cttaaaaaag agggggagta tgctcagaaa
      661 cagtgggaat cctgggccga aaggtatctt gttattactg ccagttgtgt ttatttgcct
      721 gataaaataa cggtagctac tctttgtgat gaattgcctc tgcagtacgg ccttgtaaag
      781 aaaatagatt atctttctga cagtgggcat aaggttgaag cctctttacg tgtcttgcta
      841 agggagataa cggataaatt ttgtcagtta cctgccgctc tccctgtaaa tgtgacgctg
      901 ataaccgatc agcctgattc tgaaatcaga tctgcttttg tttcagcatg ggaagcgtta
      961 tttccccaac gcgtcgttcc tgacaatatc gaggttactc ccgacttttc catggggtgg
     1021 gttgatgagc gtctgaaaca accagtgctg actgtggatt tgatactggt aatacagctt
     1081 aatgggggaa atgcatactc tgacggactg gctgcacttt tgttgaccag cgatgatgtt
     1141 gcgcaaaaat ataacctgcc gcatccagcc cgtctgctac gcccgatgtc gctggatatc
     1201 aacaagttta atgatgaatt cacacttttc cttgaaaccc agacagctgc ctgtcgtacc
     1261 gctcgtgtac tgggggactg ttatcactgg gaaaagattg ctgcaccgct gatgactatt
     1321 ggaaaccagt atggtgccgg atgggaaccg tccggacgta tgttactgga aaaatggtgc
     1381 ggtatcccgg gacctgctgc tccgtggctg cttactgctc tggcagccga tctggtctgt
     1441 cttggtaatg attctctcct gactttattc tcatccggag aagaacattt tatcagtact
     1501 gttacatcag gaaaccagaa tgaataaact ggcctgtctg tctggtcgct ttggacgacc
     1561 ggggattgta tttattggtg tggctgcgct ctggtggctg ataacccgat atggagcatt
     1621 tctgggggca gaaacacgcc gggatcaaat actactgctg atattgctga gtctggggtt
     1681 gctctttgtc tgttacctgc ctgtgatgaa aaaatatgtt caggaactga catatcgacg
     1741 tcgggcccgg aaggagcaaa ggttacctga cgatgaagag cgactggctc agacgccacc
     1801 gcgctatgtt actgttcagg atattcgtca tactctgcgg cgccagtatg gcagattctg
     1861 gggacgtaaa atacgcattc tgttgataac aggtactgcc tctgaggttg agttgctgac
     1921 cccgggcctg acagaacagt tctggcagga agagcagggg acattgcttc tctggggagg
     1981 agacccctcg cagccggaaa acgcagactg gctggctgcg ttacgccgtc tgcgttaccg
     2041 gcctgttgat ggcattgtct gggtaacttc cggactgagt gaaacattat ccgcaccact
     2101 gacagaagat gccctggaca gggtttcccg tgcggtcagt tcatgttgtg agcgccttgg
     2161 ctggcgtctg ccgctgtatg tctggtcact gcaggagagt ccggatgagc gagggcgtat
     2221 tactcagccc gtgggatgcc ttctgccggc agaatgtagt tctgacaaac tgaaagcaca
     2281 actacaggcc atgctccccg ggcttgttgc tcaggggata cagcagatat gctgtgcccc
     2341 tcggtattat tttttgttgt cgctggcaga gcgattcaga cgaaatattg atgctgtggt
     2401 ggagccatta tccgtattac tccgtccata ccgtcagttg ttgctggcgg gtattgtttt
     2461 cagtccggca actgtcggcg gtgaacgaag tgtcagacat cgctggcgaa tggataaccg
     2521 ctgggaggcg ttgccggaga cagtgcagca attacctgtc cggctacagc cttcccgcac
     2581 aggtcataac tggcgcagaa gtctggccgt tatggcggcc atactgatga tggctcaggg
     2641 aacaggaatg gtggtttcct ttctggcaaa ccggagtctg gttgctgaag tgcaggaaca
     2701 gattcgtccg gctcagaacc agcaactgtc tcctgcagaa cgtctgcagg cgttgctgaa
     2761 tctgcagaaa tccctggccc gccttcagta ccgggaggag cacggggcgc catggtacct
     2821 gcgggccggt atgaatcaga atgcagacct gctcgcggtc gtaatgcccc tttatgcaca
     2881 aaatgcacat cttttactgc gtgatgccgc tgctgctcat cttgagcagc agctcaggac
     2941 atttattcgt cttcctccgg acagcccgca gcgggggaaa atggcaaaag ccgcatatga
     3001 ccagctcaga ctgtatctga tgctggcgca accgcaacat atggagcccg cctggttcag
     3061 tcgtacgctc atgcgggaat ggccacagcg tgacggtgtg agtgccgttt tctggcaggc
     3121 taatggcccg acgttgctgg cgtattatgc ctccgggatt attactcatc ctcagtggaa
     3181 gctgacggct gatgaagagc tggtcagcca gagcagaact ctgctgcttc gccatctggg
     3241 gacacagaac agcgatgcca tgctgtatca gaaaatgctg gcacgggtgg cacatcagtt
     3301 tgcggatatg cgtctgacgg atatgaccga tgatactgat gtctcccgcc tcttttttac
     3361 ggatgaagtg gttcccggta tgttcacccg ccaggcctgg gaagaggccg tattgccggc
     3421 gatagacacc gtgattaatg agcgtcgtga agaaatggac tgggtactga cggatggcag
     3481 gcagaaggca ccgtcaccgg tatcgccgga agcactcaga cagcgcctga caacgcgcta
     3541 ttttgctgat tttggtaatg cctggctgaa ttttctgaac agtctgcact tgcgtaaagc
     3601 tcagacgctg tctgatgtga cagagcaact gacgctgatg gctgatgtgc gccagtcccc
     3661 cctggttgca ctgatgaata cgctggcggt tcaggggcgt actggtcagc cccgggaagc
     3721 ggttactgat tcactggtta aatctgccag aaacctgctt tcacaggaaa aacagccagt
     3781 gacagtaccg gaaagccggc ttcacggtcc tctggcgacg actttcgggc cggtgcttgc
     3841 actgatggat aatcagaaca acagcgcgga tctgctgaat ctgcagacgt atctgacccg
     3901 gattacacag gttcgtctgc gtctgcaaca gattgccggc tcttctgacc cgcaggccat
     3961 gatgcagatg ctggcacaga cagtattgca ggggaaatcg gttgatttaa ctgatacccg
     4021 ggattacggc agcctggccg ctgccggact cggacaggag tggtatggct tcggacagac
     4081 ggtctttgtt cgcccgatgg agcaggcgtg gcagcaggtt cttacacccg cggcagaaag
     4141 tctgaatacc cggtggcgta ctgcggtggt ggatggctgg aataatgcat tttccgggcg
     4201 ttatccgttt aaaaatgtca gcagcgatgc ttcacttccc ctgctggcaa aatacctgaa
     4261 tacggacacg ggacgtattg cccgttttct gcagaataac ctgagtggtg tgttgcgcag
     4321 ggaaggcaat cgctgggtac cggataccat taatacccgg gggctgacat tcaaccctgc
     4381 atttctgaaa gccatcaata ccctgagtga aatagcagat gtggcgttca ccacaggtaa
     4441 cgcggggctg cattttgagc tgcgcccggg aactgctgcc ggtgtgatgc agacgacgct
     4501 gataaccgat aatcagaaac tgatttatgt taatcagatg ccggtatgga agcgatttac
     4561 ctggccggct gatacggaag cacctggcgc cagtttaagc tgggtaagca ctcaggccgg
     4621 tacccgtcag tatgcagacc tgccgggaag ctgggggctg atacgtctgc ttgagatggc
     4681 ccgacggaaa gccgcaccgg gcgttgccag tggctggagc ctgagctggc aggcgcagga
     4741 cggtcgtatg ctgaattaca cactgcgaac tgaagcgggg gaagggccgc ttgtattgct
     4801 gaaactccgc aattttgttc tgccggaaat ggttttcgaa ctctccggta cgtcagcgtt
     4861 tacaggcaat gacgaagatg ccggggattc cgtggaggag actgactgat ggcttccatt
     4921 cattcgctcc tcagtgcatg ccagacgaca ccccgggatg tggctgaacc ggctcaggtg
     4981 cgtattgcgc tgtgggataa atggcttgct cccgtgacgc cagataatcc cgcaggtgat
     5041 gatgcggggt acgatgatga ttttcagcag atgcgtgaag aggtcaataa actctccgga
     5101 gccgatgccg gaattgtcag ccagcttgca gaaaaattac tgaccaccag aaccaaagat
     5161 atccgggtgg cgacctggta tatctgggcc cgtctgcggc aggatggtga aaaaggtctg
     5221 gccgacggtc tggagctgct gaccggtttg ttgcagcgtt ttggagagca cctgcatcca
     5281 caacggtcac gggccaggaa ggcggcgctg gagtggttat gcagcgcccg catactggac
     5341 agtctgtctc tgtatccgga ggtggtgaag gcggataccc tgcgcattgc cggggcgctg
     5401 tggctggcag aacagacctt tacggatgag gcttccgccc cggtgctgaa cgggttatat
     5461 caggcacttg aaaatcgcct gatgaaggcc ggtggtgttg atgcagtggt gcctcaggag
     5521 gctgccgcgc cggcaccgac ggtaacatcc ggcagcgtca tggcattatc tgccatcacc
     5581 tccggacagg aactgctgtc acaggcccgt gttctggcaa aatatctgcg tgaccagcct
     5641 gagggctggc tggcggctca tcgactgatg aaaagcgtcc ggcatgacac gctgcatcag
     5701 ttaccgccgt tatctgctga tggacggacc cgtattgccc caccggggcc ggatcggcgt
     5761 gcgtcactga aacggttgta tctgcagcag aactggttgt cactgcttga acagtgtgac
     5821 gatatgtttg cccggggagc cagtcatctg tggctggacc tgcagtggta tattcatcag
     5881 gcgctgttgc agaccggaaa agaaaattat gctgccatta ttcagtatga cctgaaagga
     5941 ttgcttctgc gcctgccggg gcttgagaca ctggctttta acgacggcat gccgtttgcc
     6001 gatgaagtga cgctaagctg gatacagcag caggtgatgg agtgtggtga acgctgggcg
     6061 gaagaaccgt ctgtaacgat aacggcagcc cccggagata acgacatttt atctcttgag
     6121 ccggaagccc tgcagattgc ggataatgaa ggaacagaag ccgctttaag ctggttacag
     6181 gcacgtcccg gtattcagtc tgaccgcagc aactggctac tgcgcctgtt gatggcccgt
     6241 gttgccgaac agactggaaa aaatgacctt gccctgcatc tgcttgcgga gctggatgaa
     6301 cgtgcaaccc ggctgaccct gtcacaatgg gaacctgaac tggtatttga ggtaaaagcc
     6361 cgccgcctga aactgttacg tatgaagtcc gcaaaaactg aaagtgacag ggtgcgtctg
     6421 cagcctgata tggaacatct tcttgccggg ctcatcgcca ttgatgctgc cagagctgct
     6481 gtgttgtgca atccggggag ctcatgagat gtctgtcatg cattatcttc aggacaaaac
     6541 atttcaggat atagttaaaa atctggtgtt accgcttctg tcgggcatta ttattcctct
     6601 tgtcaaatgg tttgtgcagc attatggcta tgcgccgaat atcaggaaat acagatttga
     6661 gaaaattcct gtatctgaaa aggagtccat tcttgcccgt attgacaaac tgacaaaaga
     6721 gccaacaacg aaaaatacac tggtaagaat aaaatactgt tatgaacaga tggggatata
     6781 tcttccgata tggtgttgta ataagctgat ttgttttata tccgacagaa atgtcagttc
     6841 agtggataac agactgcatt gttttcttaa atattctttt gtcggtatat tttcagatgg
     6901 caaatttact gttaatactc gtcgtgtgca taaaggttac cggatgatag cagtgtttgc
     6961 ggtgttttct ttatgtgttc agtttacggg agggatattt acaaccatgc catttctgtc
     7021 ggggggtaat acagttttat ttatgttatt ttccctcgtt tattttatca tgatatttct
     7081 gacagttata ttcacatgta atctgattaa tgaaatccgg cttgccgttc agttcgggcg
     7141 attgtttgag gcatggctga agtcagagcg cgagtctccg gaacagttag ctttattctg
     7201 agaagataat ggacgattta accctgcgtt attttgacgc tgaaatgcgt tatctgcgtg
     7261 aagccggaaa ggcgtttgca caggctcatc ccgatcgggc agcgatgctg gatctcgata
     7321 aagcgggtac gcctgatccc tgcgtggaac gcctgtttga aggttttgcc ttttccatgg
     7381 gacgactgcg ccagaaaatc gacgatgatt tgccggagct tactgaaagt ctggtcagta
     7441 tgctctggcc tcattacctg cgtactatcc cgtccctgtc ggtggtggca ctgacccccc
     7501 ggttatccgt catgaaaata gctgaaaccg taccagccgg actggaagtg acttcccgcc
     7561 ctgttggccc cggaaataca gtctgtcgtt accgtaccac ccgggccata ccactgaatc
     7621 cactggcagt ggagaatgtc gttatgacga cggagccgga cgggcgctca gtgctgaaaa
     7681 taggttttgc ctgcagtgaa ctggctgact ggtctcaggt cgatttgcat cgtctgagtc
     7741 tgtatctggc agcagaagcc ccggtcagca gcacccttca tctgatgatg acaaagcgtc
     7801 tggctgcgct gtatctgagg ctgccgggga atgatgagcg gatccgcatt gacggatggt
     7861 tttcacccgg cggttttgca gaagaagatc ggctgtggcc gaaaggtgac agtgctttca
     7921 gtgggtatca gttattgctg gaatatttca cctttcggga aaagtttatg tttgttcatc
     7981 tgaacggact ggagaatgtt tctctgccgg caggtatttc cggatttgat cttgaggtgg
     8041 tgctgagtca gccctggccg gcagatttac ctgtgacaga cgatgccctg tgtctgcatt
     8101 gtgtgccggt gattaacctt tttacgcttg aagccgatcc actcatcatc aatgggctgg
     8161 agagcgaata cctgctgcgc ccgaagcgtc tgcaggatgg gtatacggag atatattcag
     8221 tggatgcggt gacgggctcc gggcgaaccg gcagtgcaga gtacgtgcct tttaccagct
     8281 tccgtcaccg tgggggaatg ctgcgccatg atgcacccga acgttattat catacccggg
     8341 tgaagcgggg tgtcacgggg atgtatgaca catggcttat ccttggcggt cagcggtggg
     8401 aggtggatcg gatgccggag cgggaaacgc tttcattacg tattacaggt accaatggtc
     8461 agcttccccg tcgtgcgttg cagagtacgc tgcttgatcg gtgtgaacag gtactgcaga
     8521 ccccggtcag tgtacgtaat ctttgtaaac ccacgctacc ggtttatccg ccgacagaag
     8581 atcgttttca ctggcgggtg atgagtcatc tggggacagg atttcttaac atgctgagca
     8641 gtgcggaagt gctgcgtggc acgctggcgc tttataactg gcgggacgat gaacttaatc
     8701 accgtcggct tgatgccatt ctggctgtac agcatcatcg tattcagcgt tttgaaaagg
     8761 gttttctgct ccgggggctt gatgtggagg taactcttga tggcaacggg tttgccggag
     8821 agggagatat tcacctgttt ggcgaaatgc tcaaccgttt cctggccctg tacgccgata
     8881 tgaatcagtt caatcagctc acactgattg ttcagcctga aggaaaatgc atccggtgga
     8941 aagaaaatca caatccgcgc ctgcccggtt aattacccgt taccggaaac agcttccgta
     9001 tattaatttt tatcgcttct gtcagttact ggaacaaagc cagccagatc agcctcccat
     9061 tggcagtggc tggcaggccc gtcaggaagc ggttcgcttt tgtccataca cgggaatggg
     9121 gtttcctgcg agtgaaataa aagatgctgt aattcctgaa gaatcccatc tgccacccac
     9181 tgtacatgtg acgtttatgg gactttacgg ggtcacgtct ccgttgccgg ctcactatat
     9241 cagtgatatt gcacagcagc gggaaggcca tgaagccgca gcggattttc tcgatatatt
     9301 cagtcatcgt ctgatcaccc agtattaccg tatctggcgc aaatattcgt acccggccac
     9361 ctttgaggcg ggggggcagg ataaaacatc ccagtatctg ctcggtctgg ccagactggg
     9421 gatccccgga tgtgcacaga atattgccac gccggtatcg cgttttctgg cgttattgcc
     9481 cctgatgttg ttacccggaa gaacagctga agggctgaca tcactggtaa cgctgctggc
     9541 tcccggaaca caggcccgtg tctggcatca tgaccgacga cggatcccgc tgaaaacacc
     9601 gctggccatg cgtgttcatc atccggtcag tctgaagagt cgcccggtga tgggcgatca
     9661 tgctacggat gtgaacggac aggtgttatt acagctttca acacagacgg gcagcgaggt
     9721 acagggctgg ttaccgggcg ggcagctgta ttctgatttg ctggcgttgt tgcacgttta
     9781 tccgggaagc cgtctggatg tacgtctgca actttgtgtt aagcgttcac tgttgccgga
     9841 tgtccggctc agttgccgac cggcagccgg tagtccgcaa ctggggcgca cggctgtaat
     9901 gagaacacag gcaaagataa cgacttcagc ggcacgcgta atgaccatca gtctggggcg
     9961 ttatcagcgg gttcaggaac attatcaacg taaggaggct caggaaaatg gcgattatcg
    10021 ctggtaagac tggttacggt ctgattattg cgttgttttc cctgtcgtta tcagggtgtg
    10081 gcctgacgca aagagtggca gacggtacgg tatctgccac gaaatccctg ttttaccgac
    10141 agataaaaac acttcacctt gatattcgtg ccagggaggc cattaacacc agtgctgccg
    10201 gtattcccct gtcggttgtg gtgcggattt atcagcttaa agacaaccgg aattttgaca
    10261 gcgcggatta tcaggcgctt tttacggggg acagtgaaat tctggcaggc gatatcattg
    10321 cacagaaaga tgtatggctg cagccaggtg gttcagtggc cgtggatatg cctctggatg
    10381 atgcggcgaa atttaccggt gtggcagcaa tgtttctgga gcctgaccgg aagaaaaata
    10441 cctggcgggt ggtgctgagc cgggatgagc ttgagccgga cacgccccgt cttattgaag
    10501 tatccggtaa caccctgacg ctgttaccgg tgaaggataa atagtgatgc cgcgtccttc
    10561 cctttatgaa attctctatg gcaatttcgc tggcgggctg gagttaaacc aggtcagtga
    10621 ggaagagcag gttattcttt cggtgctcga taacatgcag cgtattctga atacccgggc
    10681 cggaagcctt aaacatcttc cggattatgg tctgccggat atgacaagca ttctgcaggg
    10741 aatgccggga acggcgcatc aactgatgtg tgtgctttca gatgttctgc tgaagtatga
    10801 gccacgtatt aagcgtgttg acgtgaccat gcaggagcag actcagcctg gtgagttgca
    10861 ttatgtcatt gatgcagaac ttaaagacgc aggactggtt cgttacggaa caacatttat
    10921 gccggagggc agggtgttac tgcgatatct gaagcaacag cagtttgtgg ataatgtgat
    10981 gactttcagg gggtgattta tggttcccct tatccgtgtg cttaacagac ataaacgagg
    11041 gctgaatgat gaacccgtta tttaatgaca tccagatgcg ccttttttat ctgaatcatg
    11101 ctccgtattc ctggcactgg aatgtcagat tcaggccaca ggaagccgta tatattggta
    11161 atgatgcctg ccatattacc attacctgta atcagtccgg ttttcattta accagggacg
    11221 ggcagcgact gttcactgaa cgttatatca gaacactcag tgagttattg gcggttctga
    11281 agcggcggtg ggatgtgaca ccggcaatca tccgtgcggt ggaatacctt tcccgggtac
    11341 cggtattaca ctgacgctct ttccgggaat attaattatg atttgataca gcaacactga
    11401 atctgccgat gacttctgaa gtaaaactga aaacaggtaa tgacccccgg tcattaccgg
    11461 attatgctgc cctgcgcgat gaaatcagca agctgactca cccggcacgt ccggatgtgg
    11521 actggcgtta tgtggagacg ctctgcctgc gtctgtatga acacaatggc gtggaattac
    11581 agacggtatc ctggtacacc atcgcccgga tgcatacagc agggttaagt ggtctgaatg
    11641 aagggctggc tctgattgtg gcgctgaccc ggcatcactg gtcagtgatg tggccgctga
    11701 atacccacgc ccggctggaa attattaccg gtctgttcag tcgtctgcaa aaaacgctcc
    11761 gggccatgcc accggatgag cgtgacaatc tgcccctgct gtatcagaca gaaacatgcc
    11821 tgaaaacgct gagtgacata ctggcccggc atgaacttaa acagagcagt aaagtggctt
    11881 taccggaagc c
//
LOCUS       DAZGBC010000154        11741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGBC010000154 DAZGBC010000000
VERSION     DAZGBC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.155"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..663)
                     /locus_tag="ACHK1J_004037"
     CDS             complement(<1..663)
                     /locus_tag="ACHK1J_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7913242.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQ"
     gene            complement(990..4112)
                     /gene="agn43"
                     /locus_tag="ACHK1J_004038"
                     /gene_synonym="flu"
     CDS             complement(990..4112)
                     /gene="agn43"
                     /locus_tag="ACHK1J_004038"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO7913243.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINLDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFIARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     IQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVIEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFRARGWGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGY
                     NGQATLNVTF"
     gene            complement(4445..5317)
                     /locus_tag="ACHK1J_004039"
     CDS             complement(4445..5317)
                     /locus_tag="ACHK1J_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7913244.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            5583..7139
                     /locus_tag="ACHK1J_004040"
     CDS             5583..7139
                     /locus_tag="ACHK1J_004040"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO7913245.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAALDDGIITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCGVQK"
     gene            7136..8351
                     /locus_tag="ACHK1J_004041"
                     /pseudo
     CDS             7136..8351
                     /locus_tag="ACHK1J_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            8471..11632
                     /locus_tag="ACHK1J_004042"
     CDS             8471..11632
                     /locus_tag="ACHK1J_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO7913246.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPVFPENASGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAAN
                     KSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQ
                     MNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIESEKEKKDFVKLFGEYLRAENVLQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGASLVTGAQTPPCLNKQA"
BASE COUNT         3013 a         2973 c         2929 g         2826 t
ORIGIN      
        1 ctgatatgcg ctccacaggt ccttaccgta atcctcccgg cgtcgtggtg tcagaatgtc
       61 ggcagtggtg acgggttgat gttcatcacc ataacggtaa gtcagcgccg cctgtgccag
      121 tgcctgacgt gccggtggcg gcagaaccag agactgcatg gcatcacgct tttcctcaat
      181 ccggtcaaaa acgcccacta cctcgtaagc cccttcaatg actctgtcca ccacatttcc
      241 ccggtggggc acacggactt cccccagaga ctgaccgcag acgcagccgt tctggcagac
      301 gaatctgaag taacccggca gcatctggta gctggaggta ccgtcatggg agttgagcag
      361 aataatttca ggcacatgct gaccggttat ctgtccggcc cgccgcagac gcagcatgtg
      421 tttggtatat tcccggcggc ccgggtcacg tacgcgggtc tggcaggcga agaatggctg
      481 aaagccttcc cgctgcaggc tttccagcac ggtgatggtg ggaatccagg tatagttctc
      541 gcttctggat gtgtgtttct cttccccgaa tacgctgggg acgacctgca tcagttcttc
      601 acgagttaag gggcggtcac ggcgtatctg gttcgcataa ccaaaacgac tggctaatcg
      661 catgatttat tccttatgaa tgagttagcg gtataaatga aaaagccgcg tctcccggag
      721 aagacgcggc ctgatggggg aaatgaatga cgtttattgt ctgagaagcc cttcactggc
      781 gagctgcgta tttttatgga aggagatcac ccaataaccc catcgggatc gggtaataac
      841 cggtcagact gtgatcgatt cagatgccat ttggcagcaa aagagccttc ggcaataaac
      901 gtgctgtaca acccggcata tctgttgctc cctgaaagta aaaagccccg gtcatgatga
      961 ccggggcctg aaggggagtg acctgattat cagaaagtca cattcagcgt ggcctgaccg
     1021 ttatagcctt cagcgctgct gccgctgacg ctgtgggcat aaccggcctg aacgcccagg
     1081 gtgatatttt cccggacacg ggcttccagt ccggcctgca ggtccagtga tgtgccattc
     1141 tgtgagggag agaacgtcat cccgctgcct gcagtggaag tccccacacg catatctccc
     1201 cgggagctga aggtgcggat aacggaaggc tgtacccacc agttcaccgg cagttcacgc
     1261 acactgtgtt ttgcactgtc acgcaggggg gcacgggatg aggtgccttc accaaaggtc
     1321 atatcgttgt ggctgcccag acggaaaccg gcacgcacat gctgtgcact gccatgcccg
     1381 aacttcacat aaccggcgtt gtccttaccg tcatccagcg aaagcccctg ccaggtgtac
     1441 tgcagccgtg gctccagcat caggttgtca gtgatactga agggcagacc ggtttccagt
     1501 gagcccagcc agccccagcc ccgtgcgcgg aagtcgttat tgcccgatga cgctttcata
     1561 ctgtggcggg ttccctgtgc cacaatgtca gcccacaggc cggaggaggt gtgtgtcagg
     1621 ttcatatatc cgcccaggct gccggcatca tcccggaccg tgccggcgcg ggagccgtca
     1681 tcatccttaa catcaacgga agaatggcct gcagcaccat acactcctgt cgtcagtgac
     1741 ataccggcga cctctgttct gagcaggtca ccctccagac ggacgaagcc atagctgccg
     1801 ctgctttccg gcgtggcccc acgggcaata ccgccgttgt tatcgtgacc gagatggccg
     1861 ccctgaatgc tgagacggac gctgttattt tcaccgttta caccggtctg atggctgcgg
     1921 gagcctgcca gaatccggtc atagtccatt gcctgtgtca gcatggaggc gtacaggggg
     1981 acttcagcac gataacgttc ttcactgcgc agataccagc tctcatcact gtcccggttg
     2041 agggtgtagt taaaggcccc ggcctgcagc atattcccct ggacaaaagc cccttcctcc
     2101 gtggtggcac cgttaatggc ctcaatcacc tgaataccct taccggtggt cgccagcccc
     2161 gtaccactgt tgccggcgtt caccagattc aggatggttt ttccggttgc cctgccaccg
     2221 tcaatgacca gtctgtcagc attgttctgc gccatatccg ggcgtacacg caggctgatg
     2281 gtgccattct gcccgttcag gtttttcacc tgcagggttg tcggaacgaa cttccctgtg
     2341 cgggcggagg tgaaatgaat ctgtccggca tggctgaggt catccaccac cgactgaacc
     2401 gtggcgttat cggggatatt ccaggtggca ccggatgtca gggtgacatt cgtggggtca
     2461 atggcaccgt tcagcactgt gctgccggtc agcttcaggg ccgtgccgcg ctgagcgatg
     2521 acatccgtgg tgacggtact gtcgttcagc gtcagcgtgc cttcattcag gttgacggtt
     2581 ttctggatga gtgtggtgtt gctgacagtg agtgtgccac ttcctgattt ttccaccctg
     2641 ccgttaccgg taagagcgcc tccctgcagg agtgcatcac cttcatggat ggtcagggta
     2701 tcgttattca ccgttttccc ggaaagggta agcgtggcac cgttattcag cgtggtggtg
     2761 cccgccagcg agccccctct ggcgatgaac agtccgccat ttaccgtggt atccgtggcc
     2821 gtatcaccgg cgttcagcgt gaatgaactg cctttttcca gcatcagggc atccgcctga
     2881 ccgcctccga tacggaatgc cgttccgtcg ctccgggtac cactgacagc ggcaccggaa
     2941 tcggccagca gtacaccgcc attccccatg gatacggtgg tggcggcacc accgttgcgg
     3001 acatccagcg ttccgccatc atccacacgg gtattagtgg ctgtgtgtcc gctcagcaca
     3061 tccagacgtc cgccattttc cagtacgaca ttatcagcct tacccgccac aacggagaat
     3121 gctcccaggc ggtttgtacc ggtgacggtt gcagctgtac tggtaaccag tgcaccgccc
     3181 gtgttctggg tgacatcaga cgctttaccg ccggcattca cctgcagctt tcctttctgg
     3241 ttgatggtgg tatgcgcggc agttcctcct tccttaatca cctgccagcc atcacggttt
     3301 atcagcgtct ctgttgccgt gccaccgttg tgtacatact ggttaccccc ctccagccgg
     3361 gtgttatgtg cctctccgtg taccgtctgg tcaccaccgg cgtaaatgag ggtgtcccgt
     3421 gccatccccg aagaccagat aacctgccgg ccatttttgt tgatggtggt ggattcagcc
     3481 gtccctccga ccatctgacc gctggacaca ttttcagact ccggaccacc ttctgcaccg
     3541 gtgttgacga tggttccggt tgccagtccg ccatgtttta tggtctggta accatccagg
     3601 ttaataattg taccggcagc tttccctccc ccgtgtaccc actgctcacc tctgttatcc
     3661 agcgtggtgt tcaccgccag tccgttaagg ctctgccctc cgccatcacg aataaccgta
     3721 tcactggcag ttcctcctgc ctgcacaatc tgacgaccat ttgcggtgac agtggtgttt
     3781 ctgcctgtgc cacccgcgtt tatccattgc ccgccggtgt tttcgtcact gtccggcccc
     3841 agctcaagcc cggtactgac agtcacgcca tcagctgttc cggatacaaa ctggttgtca
     3901 tggtttacca gtgttccgcc attcactgtt tcacccgggt gcacaacgat gtcagcagcc
     3961 agcaccggga gtgacgtgac cgcggcaaga gacagtgcaa ccgccacacc gccacgttta
     4021 ccccgtgcgc gggccagttc ggaggcaacc acgaaagcgc ccgtaatgtg attccatacc
     4081 agcctgtagc aggtattcag atgtcgtttc atcagcattt ccttaatcag agtgagggtg
     4141 aataaaaaag ccggtatcca cagatgtgga taccggcagg taaagggtta cagtgccaga
     4201 ggtccggctg acggaagttt ctgacgtgtg gctctgttgt tccctgaatg tctttctgta
     4261 cgtatctgag catgttactg acaaaggtta ttattagctt atcgatcgat ataaacgatc
     4321 gataaaatag gtatcgatcg ttttattcta ttatttggct attgcacgga caagagagac
     4381 agtaacgaaa acagcgcttt tgtgagcaga ctcagaggca gggaggacat catccacaac
     4441 aggttcagaa gaagatccag ttccataccg cgcgggcaac agagaccacg gtgtcacgaa
     4501 cagcgcgcag ggccgtacgg acaacagagg caacacagac gctctccgct gtgtcaaata
     4561 tccggtccac cgcaccggta aactgttcac gggcctgcga gcggacagac tccgtgcgca
     4621 gctcgtcctg caggcgggtc atcaggggac tggcggcatg gtcgggaagt gctgtcatga
     4681 gcgcactgac cagcgtatcc agttcccagc cggtgcgggc cgatacggcc acaaccggat
     4741 gtacgggccg gaacagccgg aataccgcat ctgttttctc acgaatgttc tgttcctgtg
     4801 cgggagaagg ctgaatgccg gccatatccc attcatggca gggctccgtt ttgtcggcct
     4861 gcgtcaccac aaacagcacc tgctgatgtc cgcactgcag gatgtgtcgc cagaaatact
     4921 catccacaga cagggcccgg tcatcggctt taatcagcca cagcaccagg tcaagttcag
     4981 gcagaatgtc acggtacagg gcctcatact ctgcatccct gtcccggctc tcgcccaccc
     5041 cgggcaggtc agtgataacc atgctgtgac cgtggccact cagacggaag cgccgtactt
     5101 cccgggtgcc ggcgtgaaca tcactgaccg gggtgacctc cccctgaaac agtgcattac
     5161 agagtgagga tttaccggcc ccgcttttac ccataatgcc aatcacgggt tcgtgacggg
     5221 tgagtttgcg cagatgttcc aggatgtgac gggaaagtga gtaaggcagg gaggagagcg
     5281 gtttttcaat tgcctcaatg gcatcagacg gattcataca tgtattcccg gtgaaaaaag
     5341 acaaaacccc gcgcaccgga aaggtggcgg gggactcaca acgataatat ctgtctgaaa
     5401 ttattttgaa tgtacccggt gcactgtaaa agaaaggcag tgttccgttc ctcctccctg
     5461 tcatgaataa cctggtgtat ctcacaatat ctgctgactt gctccggggc tgcctgaacc
     5521 acaagattgc tgtagtattg cccccggaga ttcagttcca gaccaggaaa aacaggttaa
     5581 aaatgacaag tattcaacaa agagcagaac tgcaccgcca gatatggcaa attgctaacg
     5641 atgtcagagg ttcggtcgat ggatgggatt ttaagcaata cgttctgggc gcgcttttct
     5701 accgttttat cagcgaaaat ttctccgggt atatggaagc cggtgatgac agcatctgtt
     5761 atgcggcact tgatgacggc attattactg atgacattaa agacgatgcc atcaggacta
     5821 aaggctactt catctacccc agtcagcttt tctgcaacgt agctgcgaaa gcaaatacca
     5881 atgacagact gaatgcagat ttaaacagca tcttcgttgc tatcgaaagt tctgcttacg
     5941 gttatccttc agaagctgac atcaagggtt tgtttgctga tttcgatacc accagtaacc
     6001 gcctgggtaa caccgttaag gataaaaatg cccgcctggc tgctgttctg aaaggggttg
     6061 aagggttaaa ccttggtgac ttcaacaaac atcagattga cctgtttggc gatgcctatg
     6121 aattccttat ttctaactat gccgcaaatg ccggtaagtc aggcggcgag ttctttacac
     6181 cacagcacgt ctctaagctg attgcacaac tggctatgca cgggcagacc cacgttaaca
     6241 aaatctacga cccggcagcg ggttccggtt cgctgttgtt gcaggcgaaa aagcagtttg
     6301 atgaccatat catcgaagaa ggcttttttg gtcaggaaat caaccatacg acctataacc
     6361 tggcgcgtat gaacatgttt ttgcacaaca tcaactacga caagtttgat atcaagctgg
     6421 gcaatacgct gactgagccg cacttcagag atgaaaaacc gtttgatgcc atcgtttcta
     6481 acccgccgta ttcggtgaaa tggactggca gcgatgaccc gacactgatt aacgatgagc
     6541 gttttgcccc ggctggcgtt ctggccccca aatccaaagc tgactttgcg tttgtattac
     6601 atgcgctgaa ctatctttcg gccagaggtc gcgctgcgat tgtctgcttc ccgggtattt
     6661 tttaccgtgg cggcgcagag cagaaaatcc gtcagtatct ggtcgacaat aactatgtcg
     6721 aaaccgtgat ttcactggca ccgaacctgt tctttggcac caccattgcc gtaaacatac
     6781 tggtgctgtc taaacataaa acggatacca gagttcagtt tattgatgcc agcgaactgt
     6841 tcaaaaaaga ggctaacaac aacattctga ccgatgctca tatcgaacag attatgcagg
     6901 tatttgccag caaggaagat gttgcccatc tggcgaaatc agtcgcgttt gagaccgttg
     6961 tcgctaatga ctataacctg tcggtgagca gctatgtgga agcgaaagat actcgtgaaa
     7021 ttatcaatat tgctgagctg aatgctgagc tgaaaaccac ggtcagcaaa atcgaccagt
     7081 tgcgtaaaga tattgatgcg attgtggctg aaattgaagg ctgcggggtg cagaaatgag
     7141 cgagttgagt tatctggaaa aattgctgga tggggttgag gttgagtggt tgccattggg
     7201 ggaagtctca gccttaagaa gaggcagagt tatgtcaaag ggatatttaa ctgaaaattt
     7261 tggcccatat cctgtctata gctctcaaac tgcaaataat gggaaaatag gttctatcaa
     7321 taccttcgat tttgatggcg aatatataag ctggacaaca gatggcgcaa atgcaggcac
     7381 tgtgttctat cgaacaggta aattttcaat aactaatgtt tgcggcctaa ttacattaaa
     7441 aagtaaatat ccattaatct acaaatttct gttttactgg ctaacaatag aggccaaaaa
     7501 acatgtttac tcaggaatgg gaatcccaag ttaatgagtc atcaggttga aaatatacct
     7561 gtaccaatcc cctgcccgga taatccggaa aaatcccttg ccatccagtc tgaaatcgtg
     7621 cgaattctgg acaaattcag cgccctgaca gcagagctga cagcagagct gacagcagag
     7681 ctgacagcag agcttaacat gcgtaaaaaa caatacaact actatcgaga taagttgttg
     7741 aatttcaaag atattgcggg ggggggtaat tacatccttc cggacgccat ggatggtatt
     7801 cggattgagt ggaagacttt gggggaagtg gctgttatag gaacaggtaa ccatgatacg
     7861 caagatgcta tagaacatgg caaatatatt ttttatgcac gtggacgtga accattaaag
     7921 ttaaatgtat ttgattttga tgaaacagca atcattacgg ctggagatgg tgccggggtg
     7981 gggaaggtat ttcattacgc taaaggtaaa tatgctttgc atcagagagc atatcgtatt
     8041 gtcccaaatg catttatgaa tccgcgattt gtatatcact atataacagc atatttttta
     8101 cctatattca aaaagcatca gtaagttcat cggttacctc acttcgtcga ccaatgtttt
     8161 taaaattccc aatacctgta ccaccttcag aagaacaagc tcgcattgtc gaaatactcg
     8221 ataaattcga caccctgacc aactccatca ccgaaggtct cccgcgtgaa atcgagttgc
     8281 gccagaaaca atacgagtac tatcgtgatt tactgttcag tttcccgaaa cctgaaaccg
     8341 tcagtaacta attgacaatt gctaccgacc gggccacctt aacatccggt cagtatataa
     8401 accattttta cacgccggaa gtcaccctta actcccttcc ggccccgcca gacggcacaa
     8461 aggatgcgct atgattcttc agacacacac cattgctgaa accagtaact ttatcgttct
     8521 tgataagtac atcaaggctg agcaaacagg cgacagctac cagagtgaat cggacctgga
     8581 acgtgaactg attcaggacc tgcagaatca gggctatgaa tttatttccg taaaatcaca
     8641 gtcggcgatg ctggctaatg tccgggaaca gcttcagagc ctcaatggcg tggtgtttaa
     8701 tgacagcgaa tggcggcgtt tcacggagca gtatctggac aaccctggcg atggcattct
     8761 ggataagacc cgtaaaatcc atatcgacta tatctgtgac tttatttttg acaacgggcg
     8821 tcttgagaat atctatctga tagataaaaa gaatctcatg cgcaacaaag tgcagattat
     8881 ccggcagttt gaacaggcag gttctcacag taaccgttat gacgtcacta tcctggttaa
     8941 tggtttgccg ctggtgcaaa tcgaactgaa aaagcgtggc gtggcgattc gtgaggcttt
     9001 caaccagata catcgttaca gcaaggagag ttttaacagc gaaaattccc tgtttaagta
     9061 tctgcaactg ttcgtcattt ccaacggtac cgatacccgt tactttgcca acacgacaaa
     9121 gcgcgataaa aacagttttg acttcaccat gaactgggcg aaatcagaca acacgctgat
     9181 taaagacctc aaagatttta ccgccacctt tttccagaaa cataccctgc ttaatgttct
     9241 ggtgaactac agcgtttttg acagcagtca gacgctactg gtgatgcgac cgtaccagat
     9301 tgctgccacc gagcgcattc tgtggaaaat taagagctcc tttacagcga agaactggtc
     9361 aaaaccggaa agcggtgggt atatctggca caccacgggt tccggtaaaa cactgaccag
     9421 ctttaaagcc gcgcgtctgg caacagaact ggactttatt gataaagtct tctttgtggt
     9481 cgacaggaaa gacctcgatt accagaccat gaaggaatat cagcgttttt cgccagatag
     9541 tgttaacggc tcggaaaata ccgcaggcct taaacgaaat ctggataagg acgataacaa
     9601 aattatcgtc accactattc agaaacttaa taacctgatg aaagcagaaa gcgacctgcc
     9661 tgtatacaat cagcaagtgg tgtttatatt tgatgaatgc caccgcagcc agtttggaga
     9721 agcgcagaaa aatctgaaga agaaattcag acgctattat cagtttggtt ttaccggcac
     9781 acctgttttc ccggaaaacg cctcaggttc agaaacgacc gccagcgtat ttggtcgtga
     9841 attgcattcg tatgtgatta ccgatgcgat tcgtgacgaa aaagtgctca aattcaaggt
     9901 ggactacaac gatgtgcggc cacagtttaa gtctttagag acagaaactg acgagaaaaa
     9961 actgagtgcg gctgaaaatc agcaggcgtt tcttcatccc atgcgtattc aggaaatcac
    10021 gcaatatatc ctgaataact tccgccagaa gacccaccgc actttcccgg gttccagagg
    10081 ttttaatgcc atgctggcag taagcagcgt ggatgctgcg aaagtctatt acgcgacgtt
    10141 taaacgctta caggaggaag ccgctaataa atctgcaaat tataaaccac tgcgtattgc
    10201 gacaatattc tcctttgccg ccaatgaaga acaaaatgcc attggtgaaa tttccgatga
    10261 aacttttgat accggcgcaa tggacagcag tgctaaagag tttctggatg ctgcaattcg
    10321 tgaatataac gaccattttc aaatgaattt cagtactgat agcaaggatt ttcagaacta
    10381 ctatcgggac ctggctcagc gggttaaaaa tcaggatgtt gacctgttaa tcgtcgtggg
    10441 gatgttttta accggcttcg atgctccaac actgaacacg ctgttcgtcg ataaaaacct
    10501 gcgttttcac ggtctgatgc aggcgttttc ccgtaccaac cgcatttatg acgccacgaa
    10561 aaccttcggt aacatcgtca cattccgtaa cctggagcag gcaaccatcg atgccatcac
    10621 actttttggt gataaaaaca ccagaaatgt ggtgctggaa aagagttatg cagagtatat
    10681 ggaaggcttt acagatgctg ccactggcga agctaaacgc ggctttatgg cagtagtttc
    10741 agaactggaa caaaggttcc ctgaccctgc cagtattgaa agcgaaaaag agaaaaaaga
    10801 cttcgttaaa ctgtttggtg aatacctgcg tgccgagaac gtcctgcaaa actatgatga
    10861 atttgccacg ctgaaagccc tgcaacaaat cgatctcagc gatcctgttg cggtagaaaa
    10921 attcaaagca gaacattatg tggatgatga aaagttcgct gaattgcaaa tgattcgtct
    10981 ccctgctgaa cgcaagattc aggattatcg ttctgcctat aacgatattc gtgactggca
    11041 gcaccgcgaa aaagaagctg acaaaaaaga aaaatcaacc actgactggg atgacgtggt
    11101 ttttgaggtc gatttgctga agtctcagga aataaacctg gattatatcc ttggcctgat
    11161 tttcgaacac agcagacaaa acaaaagtaa ggacgaaatg accgtagagg tcaaacgatt
    11221 aattcgttca agtctgggga accgtgctaa agaaggcctg gtggtcgatt ttattcagca
    11281 aacgaacctt gatgatttgc cggacaaagc cagcatcatt gatacgttct ttacctttgc
    11341 tcaacgcgaa caacaacgtg aagcagatgc attgatcaaa gaagaaaatc ttaatgaaga
    11401 agcggccagg cgctatattc gcacgtcgtt aaaacgcgaa tacgccaccg aaaatggcac
    11461 agaattaaac gagacattac caaaacttag tccgttaaac ccgcaatata aaacgaaaaa
    11521 acagaccgtt tttcagaaga ttagcgcatt tattgagaag tttaaaggcg tggggggggc
    11581 atctttagtt acaggcgccc agactccccc ttgcctcaat aaacaggcat gaacactgac
    11641 tgctattgta aggcgttaac ggagtccgta ttgacgctta cgctcaagtc acgactaata
    11701 caactaaatg aatgattaac agctcagcta aacggagctg t
//
LOCUS       DAZGBC010000155        11367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGBC010000155 DAZGBC010000000
VERSION     DAZGBC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.156"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            86..991
                     /locus_tag="ACHK1J_004043"
     CDS             86..991
                     /locus_tag="ACHK1J_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7913247.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(1025..1627)
                     /gene="cobO"
                     /locus_tag="ACHK1J_004044"
     CDS             complement(1025..1627)
                     /gene="cobO"
                     /locus_tag="ACHK1J_004044"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO7913248.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1637..3289)
                     /locus_tag="ACHK1J_004045"
     CDS             complement(1637..3289)
                     /locus_tag="ACHK1J_004045"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7913249.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVSLQLLRNAEVSAKGVLP
                     NCQDTGTATIIASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDICAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKALL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGESMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3397..4677)
                     /locus_tag="ACHK1J_004046"
     CDS             complement(3397..4677)
                     /locus_tag="ACHK1J_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO7913250.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4838..5155)
                     /locus_tag="ACHK1J_004047"
     CDS             complement(4838..5155)
                     /locus_tag="ACHK1J_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO7913251.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5152..6522)
                     /locus_tag="ACHK1J_004048"
     CDS             complement(5152..6522)
                     /locus_tag="ACHK1J_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO7913252.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAEIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6526..7767)
                     /locus_tag="ACHK1J_004049"
     CDS             complement(6526..7767)
                     /locus_tag="ACHK1J_004049"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO7913253.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     TVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7767..9212)
                     /locus_tag="ACHK1J_004050"
     CDS             complement(7767..9212)
                     /locus_tag="ACHK1J_004050"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO7913254.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNAGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9231..10619)
                     /gene="glmL"
                     /locus_tag="ACHK1J_004051"
     CDS             complement(9231..10619)
                     /gene="glmL"
                     /locus_tag="ACHK1J_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO7913255.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10619..11068)
                     /gene="glmS"
                     /locus_tag="ACHK1J_004052"
     CDS             complement(10619..11068)
                     /gene="glmS"
                     /locus_tag="ACHK1J_004052"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO7913256.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVENKFKEMGFDRVFAPSHDLEEVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2698 a         2835 c         2849 g         2985 t
ORIGIN      
        1 attatttatt gctgctttgg ttaccattac ttcgtaataa gtaatgtatt tttgatggtt
       61 taaactattt ttaagggacg gcattatgcg agggaaaata cctaaaacgg aactgctggt
      121 gacttttgag gtcgtcgcac gtcatgaaag ttataccaga gcggcagagg agttagcgtt
      181 aacccaaagt gcagtattca gacaagtaag tgcgctggag gaatttttac gtactccgtt
      241 attttcacac tctaaaaaac gcatttttct taatgatgca gggaagtatt acctggggat
      301 agtaaaagaa acattaaata agctggaacg cgataccaat accattatga cctggcagcc
      361 tacggttcag gttattgaac ttgccgttaa tcctactttt agtacccact ggttgatccc
      421 aaatttacat gaattcacaa agttacatcc cgatattatt gttaatattc attctttagc
      481 taataacggc gattttctta atcgggagta cgatgctgtc attatgcgcg aaaatttttg
      541 cgcgccctgg gcggaagtag aatatctctt tgatgaggag attttgccgg tatgcagcgg
      601 cagtttgctt gcaatgtccg atcaaaagct gtcagtggca gaactgctta ctgagttacc
      661 gcttctgcat caaagcaccc gtattactgg ctgggaagag tggtttgctt tatctggcgt
      721 tagcagtccg ttggtcaata acggaccacg tttcgatttg ctttcaatgc tgattgccgc
      781 cgtgcgctct aatctcggcg tggcgctgtt accacgtttc gctattcaac atgatttgga
      841 tagtggcgat atggtgatcc cctgtgatgt gcctattcgt accggtaatc gttttattat
      901 gacgtggcag gaagagaagt ctgactcacc gcacttgcag caatttcgtg aatggttgct
      961 ggcgaaatcg gtcgtaccgc aggagatgta aaacctgcgc ctggcgggat gccaggcgca
     1021 gttattacca gtcgataccc ggctgggcct gtatgccgtt atcaaaagca tgtttgacgg
     1081 ggcggatttc gctgaccgta tctgccattt ttaatatctg actgtggcaa ccgcgtccgg
     1141 tgacaatgac actttgttgt gccgggcggt tttgcagaga agcgatcact tcttcggtat
     1201 ccagataatg gtaagccagc atataggtca gctcatcaag caccactaag tcgtaacgct
     1261 tgtcagccag catccgtttg ctttccgacc agacctcttt cgccgcgtcg atatccgctt
     1321 gccggttctg tgtctcccag gtaaaaccgg ttcccataat atgaaactca acccccagcg
     1381 gctggagaag gttgtattcg ccgttgtccc attgcccctt gatgtactgc gcaaccccca
     1441 ccgttttgcc atgacccacc gcgcgcgtca cagtaccaaa cgcggcagtg gatttccctt
     1501 tgccgttgcc ggtgaaaaca atcaagatcc cttttttctc tatcgccgct gcgacgcgcg
     1561 tatcgacctg ttccttcaat ttctgttggc gctgcacatg ccgttcagtt gaaattcgcg
     1621 cttccattca ttttccttaa tgtcccgcag ggcaactggc gcagcggtgt tgctgctcaa
     1681 actgactaaa gaaattattg cctttatcat ctaccaggat gaatgcgggc agattttcca
     1741 cttccatcat ccacaccgct tccataccca gctccgggta ttccaggcaa cgcagacttt
     1801 tgacatattc ttgcgccagt aacgcagctg cgccgccaat acttcccaga ttgaagccgc
     1861 catgtttgtg acaggcatcg gtgacctgct ggctgcggtt acctttcgat aacatcacca
     1921 gactgccgcc ggccgcctgg aaggtatcga cataaccgtc cattcgaccg cccgtggttg
     1981 gacccagtga accgcaggcc atgttctccg gtgttttcgc cgggccagcg taatagacaa
     2041 tatgatgctt caggtactcc ggcatcgatt cgccgctatc cagccgcgct tttattttgg
     2101 cgtgggcgat atcgcgcgcc actacgatgg gaccacttaa cgacacgcgg gtgcctaccg
     2161 gtaatctggc gagatccagc atcacgtcac gcaacggacg gtttagatcc agttgtacat
     2221 gttgcgcgtg attttcttcc cgcaatgagg ctggaatata ttgccccgga ttgtgttcga
     2281 gtttttccag ccagataccg tgtttgttaa tttttgcttt gatattgcga tcggcagaac
     2341 aggagagcgc catagctatc gggcaagagc caccgtgacg cggcagacga atgacgcgaa
     2401 tatcatgagc gaaatatttc cccccaaatt gtgcaccaat accaaattgc tgactggcct
     2461 cgagcaacgc tttttccagc tcaatatcac ggaatgcttg tccctgctcg tttccgctgg
     2521 tgggcaaatt gtcgtaatat ttcgttgagg ccagcttggc aatttttaag gtttgatcgg
     2581 ctgaaagccc tccgacgaca aaggcgatgt ggtaaggcgg gcaggcagcg gtgcccagcg
     2641 atttcatttt ttcgattaaa aatgcggtca gtttttctgg ttgcaataag gatttggttt
     2701 cctgataaag cgcggcttta ttggcagagc cgcctccttt attaacacag aggaaatggt
     2761 actcatcacc agcgacggcg cagatatcaa tctgcgccgg gagattggtt tgcgtattga
     2821 cttcggtata catatccagc ggcgcatttt gtgaaaagcg cagattattt tcctgaaaag
     2881 tggtgtaaat gcctttgctt aaggcttcgg cgtcgttacc tccggtccat atctgttgcc
     2941 ctttactggc aataatagtt gcggtgccgg tatcctgaca gttgggtaat accccttttg
     3001 ctgagacttc agcattacgt aataactgca acgaaacgta tttatcatta ctactggcct
     3061 gcggatcgtt aagaatgctg gcgacctgtt gcaaatgagc ggaacgtaag aaaaaagagg
     3121 cttcataaaa tgcctgccgg gcaagcagcg ttaatgcttc aggggcgatt tttatcactt
     3181 cttcgccgtc taattccgtt actgtaatat gctgatcgct aattaatgta tattctgtac
     3241 cgtctttatt ttgtagaaac ggttcttgcc agataaatgg tttggacata tttcactttt
     3301 caccttaaac gttagtgtta cttaattgcc gtctaacttc atgatccggc ttataaaaat
     3361 gcgtagtcat tggcttcaga ttgcttaaga gccccctcag ggcagagcaa cgttcgattc
     3421 attgggtttt aatgctttac cccgacagcc ctcgccgaaa tgttcgcctt cccaccgact
     3481 caccatcgca gcgcccattg cgttgctcat gacgtttgtc gcggaacgcc ccatatcaag
     3541 gaacggatca acgcccatca gcaagatcag ccccgcttcc ggaatattga actggttgag
     3601 cgtggcggcg ataaccacca ttgaggcgcg tggtacgcca gccattcctt tcgaggtcaa
     3661 catcaggatc aacagcatgg tgatttgctc accgatggat aaatggatat tgcaggcctg
     3721 ggcgatgaaa actgtggcga aggagcagta ggccattgat ccaacgagat taaatgagta
     3781 gccaatgggt aagacaaagc tggcaatttt gggggaaacg ccaaattgct ccagtttttc
     3841 aagcgttccc ggaaaagccg cttcagaact ggatgtggta aatgccagca gggcgggttc
     3901 cgaaagggcg cgggtcaggc gtctgatgca ggggccgaca taaacgatgg ccagaccgat
     3961 aagcagcacc caaagtaata acatggtgaa ataaaattca cccataaaga tcccggcgct
     4021 caccataact gccagtcctc gttcagcaat caatgctgaa atagcagcga atacggtcag
     4081 gggagcgaag agcatgacgt agccagtgag ctttaacatg gcatgtgcca gcgaatctaa
     4141 ggcgtgaacg atggcgctgc ctttctcgcc aatcgccgtc aggctacagc cgaggaaaat
     4201 tgagaacacc acgatttgca aaatttcatt gtgcgccatg gcatcgacaa tgctggtggg
     4261 gaaagcatgc gaaataaata cttttagcgt gaagggttct gccgcgacca ctccggtggt
     4321 ttcggcaccg tgtgcaacaa aattaattcc tgtacctggc atgaagaaat ttactgttat
     4381 caagcctaag gcaattgaca gcaatgaggc gcaaataaat aaaaagagtg ttttagaaaa
     4441 aatacgacca agggctttgg catcgcccat tttagcaata cctaccacca gggtagaaac
     4501 cactaacgga gcgataatca tttttatcag tcgtaagaaa atcgtcgtga atatcgatat
     4561 ttcttgcgca tagagttttg ctgtttccgg tgaagcagtg ttattgagca ctacgccgat
     4621 aatcattcca agtaccagcg ccaaaagaat catcgtggtt aaacttattt tcttcatttt
     4681 ttcctccatc aggtgcacgg cgataaatca gaatcaggag aagtagatcc ctgaatagag
     4741 attcaggcat ctacctgcta ttacaacctt acaaatagct gaagtgttga actgcaataa
     4801 gtgacgggat gtcagttggc taaaagtatt cggattttta caaatcgaaa ctcatattta
     4861 ataaaggtgc atgttgctgt gcaccataga catcattgtc acctacattc ccggagataa
     4921 tatctcgtgg catgacgatt tttactgcgt tagaaggatc aaaccagaca atacgatgaa
     4981 taaagtcagg ttcaacatga tataagcgcg caatcagttc tggggttaat tgccctgatg
     5041 atttaactcg ctcatagttt tctttcgttt taaataaaat atctaaaacc agttcgtaag
     5101 gtccggcatt tttcgaacga atgacttgcg ccagtgagac aattgactgt ttcatgcctg
     5161 aactccttct ggcgtcacct gttcaatatg gaaatcaaaa cgcagagcgt cgttggcttc
     5221 aatcaggtga tatatggaaa attcgtatac cggcccgccc tggatatcag aaggggagaa
     5281 cgggaacgcc agattgcctg cggtagcgat gcggttttca tagccgtagt gcagcatggt
     5341 agagcgcacc agcgagcaaa cgctattggc aatttcctgg gtcggtgcaa ctacatcaag
     5401 gacaatcccc agctcatgcc cggcagtttg catcggttca tggtctccca tcaccccgtt
     5461 tttgccgtac aggtggaaat tgatgcgaat gctgtcatcg tcgagcgaca ggttacgcga
     5521 aacgctggtt ttgacttcat cgatgatttt atcgatctcg gcgatcataa tcgggtcgcg
     5581 cgttccggcg atggtcagac agcggaagcc cacccgtcgt gcaccttcca gtttcagggc
     5641 atacggcgtt tcttcatgct tagaaccgct gacgtagact tcgccgtcat tgactgcttt
     5701 aaatgtgcag cctttcaggt tcaacacgcc gccaggtcct ggcaggaagt agggatcgga
     5761 tttctcatac agtgtgtgtg cagctgctga cgtttcggta aatttacgct tcggattaaa
     5821 tgtcttcagc gtaaagccgt tgtcatcaat gatgcccatc gcacagtcag agcctgagcc
     5881 gggcgttgcg gcgattgccg cgcattcaag gattttccca caatgcagcg ccagaccttc
     5941 atcaaagcct tgcatgatcg ggagcgcagc aaagcaggcc gggtcgtaag cgcgcccgcc
     6001 cagcaccact tgcgcgcctt ctttcagcgc ccgctggaag ggttcgatgc ccatttgcgc
     6061 gacgatatac gtactttcgt caatcgcgtc gtgggttaac ggcgggacaa aatccagcgc
     6121 cgtgattttg ccgttatcca gcgcctggtg gacgatggct ttgtcaacgt ctgccgggat
     6181 caacgccatc gagaacgaga ggtgctcttc ctgggcaatc tcatgaatta tttctcggca
     6241 ccactccagg tgtggagcgg caccagaacc gccggccgtt ccgatcacca ccggtatatt
     6301 attttgcacg cctgcggtga tcatatagcg cagatcgcgc ttgacccctg cgcgatcggt
     6361 aaacggtttg cctgccccga ggtagtgggg gcctggatcg gaagagcctg catcaacggc
     6421 gatcagatcg ggtgattctg ccatcgcttt gcggaagctc tcttccggga atccataccc
     6481 gaggattgct gttggcgata agactttaaa agaacgagcc atttgttaat ccttagcctg
     6541 caacagcgcg atagtacgat tcatttcgtt gaagacgata tcgaggcctt cgtcaaagcc
     6601 catccctggc tttaccagca tacgcatagg gcgagcggca agggcgacgt ggacacaggt
     6661 gcgggcactg acatcagttt cattgcaggt gccgccctgg tacgcttcca tgctgtggct
     6721 gttgcagtaa agaaccgcat cgacgatgtt gtgaatactg cccagatccg gggttttgat
     6781 ttgcaccatg tgacaactgg cggcatcagt gaaatcaaca atatcctggt aggtgttaca
     6841 ccattcatcg gccacaattt tcacgccgga accgaggcgc gtcagctctt tggtaatcgc
     6901 ggtcagcagg cgaatttgat cgggcttgtt accggcatcg accggccctt caatgtagag
     6961 cggcaggcct tgcgcttctt tttccaggct ggcgatgtat tgcgcacagc gaagcggatc
     7021 catatcgaag atcagaccga tagtgccgta tacatcgatg tgcagggtag ggtggtagcg
     7081 tgcgctggtg cgcttgctta gaatgcgatc cgacaaccag cggacatatt cgcgcagttt
     7141 ttcaccttta aagcccagct tctcttcgac gttattaatc agcgcatggg gcagcacgtc
     7201 gatgcccttg aggatcatct tatcgacggc gatatatcga tcgtcgccgc tctgaccaaa
     7261 taatggaatg gattccgcca cgcgtggcaa ctgccattca tcacagacca cttcagtttt
     7321 cagacggccg gttgccagcg cggtagcatc aagcagcgcc tgtgataatc cgtagcgcac
     7381 ggcggtatgc agcaagttgc cgtcaatacg caatttgtcg aagaaacggg cattcggcag
     7441 gaaagcatcc acatcgcggc ctaccagtaa tggcttgata tggtcgttga ggaacggaat
     7501 aaagtgctct gcgaggaaca gtggatcgcg accacctgcc ccagaatact gtacggcagc
     7561 acagtcacct acagcgaccg cgccgttttc cagaatcaac tgtaccgata cgcactcccc
     7621 ggcctgacgt acggtgttaa acccctgtgt tactggctcc ccggtataaa aaaagccgtc
     7681 gtgacccgct ccgtttttta tcgcctgctg gtcatcgaaa tagaatgagg aatagccagc
     7741 ggtgaacagg gcctgtttta ttttcattat ggtcttccta ttaatttact gtgggataca
     7801 gcattgatgt catctacaac catctggaat gtcggttggc gtccttcaca gcgtgcgcgt
     7861 tccgcaacgt aatcgtggtg gaggtcgaga atatcttttg gcaatggaac ggctccggct
     7921 tccagtacgc gtatcgcgcc ggcgttgtca cggaccggaa gaattttgcc cgcgttgcag
     7981 gcggctggcg caaacggcac atccagtacg cccgcttcaa atgccagcac cgttccgcgc
     8041 gcaatatcac cattacccag ttcgaacact ttattcagta ctgcgcgaac ctcgctctta
     8101 atcagttcaa tttccagttc aaccgcaggg cacggcggga atttctgctc attgaccatg
     8161 ttgagcatct ggcgggaggc tttcagcccc tgaatgttgg cagcggcagt cgggatgccc
     8221 caggcttcgt gcggactttt ggtgatgact ttggtggcac cagacatccc cgcgacggct
     8281 gcgccccagg agataatagc gaacgctttc gattcgtctt ccggaaagcc gcccatccac
     8341 tggtgaaaca cggtactcaa ttcatagtcg gtatagccat agctctgaaa atattcgtgt
     8401 gcaagttcac gcagagactg gatcgccgca acatcctgcg tcaggctgcc gacctggcca
     8461 taaccaacgg tgatagattt cacaccctgt tccagtgcca gcaagccttc gattatcgcg
     8521 atagagtgcg agataaacgg cgggatcagc gtacccgtta gtgggccaaa cggttcgcgg
     8581 ttgatgcgga tcccgtgttc ttcatacatc cccattaaac ggtcgcagta ctgccagtcg
     8641 cggatcgatt tttccagcgt tacgcgtttg gcatacggaa tgttgtagga aatgccaccg
     8701 ccttcataac tggtaaagcc actggccatc gagatttcag ccagcagacg cgcatctggc
     8761 gtgccgtggc ggatctgtag cggtttttgc agggtttcgg tgaggcgacg gcaagctgcg
     8821 acaccatggt taactaccgg taaaccgttc agtttagagg ttccggcttc gatggatttt
     8881 ttaatcccta cagccgcttc ttcgtaccga ttcagacggg tatatgcatc gatggtgctt
     8941 ggcagcagat cgcactcttc ttgtagtgtt ttcagcagtt cgatgtgttc gtccatcagc
     9001 gccacacctg cgcgcggttg gctaagggtt ttaccttcct tgtctgcttt cagcagcgca
     9061 agagagaagc gtttatgctc agggatggtt tgttggtatt taacgccatc ttcaaagttt
     9121 tcaacatcct tgccggtttc ccaggttttc agtacctgtt gccgctcggt catgaattcg
     9181 tcatgggtta atttcttatt tcgaagttcc attgattgca gccttaattg ttatagggtt
     9241 aagcattgaa tactggtgcg cgcggcggcc agcggatcga ctctggctac gtttgccagc
     9301 agtggtaata gcccccggga atcgcggtaa tattcaaatt cggtcggtaa gagaatgcgt
     9361 ttaccgtcgt cgtttaactc gcggtatttc aaccagtgat gcatatcgaa ctgactggca
     9421 cgcgagagcc atccaccgga acccacgact ttacgcacgg ttgtcaggtc acgtcccatc
     9481 tgtaaatcga cattgccgac gcaggtgcag acctcttttt tggtgcccgc atgtctttcg
     9541 gtggcataac ccacacacag tccggccagg acggtatcga aatatttctc ttctgcattt
     9601 gcgggtaagt agtcgggatg agcgactaga tgacggaggt aatgataaaa agcgtccagc
     9661 tgtgcgggtt gctgagcaaa attcacattg actaactctt tcgcactttc accaaccacc
     9721 actgccgaga cgcgcattcc cagatcgcct tccaccgtcc gttttacgaa gggttccggt
     9781 acgccgtgta aaacggtgtc gggcgagagc gtgttggcac aggcggaata aacatcggtg
     9841 gttgcgccgc ccatgtcgat cagcatgaac tctttccagc tgtgatcgac gttactgatt
     9901 gccttgacca gttcaaagac cgtccatggt gtcggcattg gttcttcgcc tgttttatca
     9961 acaatgacat ccagcccttt ccctttaacg atgcgtttaa ggaatatgtc gcaaatagcc
    10021 tggcgtgcgg ctaaagggtt cggatggtca agatcgggca gaacgttgtc gacgatggtc
    10081 aggtctttat gcccgaggat ctcctgcacc tcgtcctgaa tatcccggtt tccggcataa
    10141 ataatggcgc agtcgagttt ggattctgcc agcacacggg cgttattcag tccgtagctt
    10201 tcttctccgc cgtcggtgcc gccggtgaac aagagaatat cgggctgcgt ttcttccagt
    10261 gcctggatat cacggcggtt cagcttgtag gcgtaatatt gcgcaatttt agcacccgca
    10321 gagtgagcgg ttactttcgc ggtttccagg gtgatagaag gcaccaatcc cattgcggca
    10381 accgccagcc cacctttggc agaggaggaa tacttcaatg caacctcacc gctgtttaac
    10441 agggggaggg cattatctac gtttaacacc tggttcaggc tggagaaaaa ccccttcgcc
    10501 agatgatggg tggtggttgg ggtcagtacg tggttaacca acgttaacgc gtccccctcc
    10561 cgggtaaaga gggctgcttt ggtccacgtc gatccaatat cgacagagac gatttgcatc
    10621 agatagcctc ttcaagaata cgagtatcaa cgtcatgacg ttggttgata tcgtgggcca
    10681 tcagctgaca aacctcttct aagtcatgac ttggggcaaa tacgcgatca aatcccatct
    10741 ctttgaactt gttttccacg tcagcgaagt catgttttcc taccaccagg ttaccgccca
    10801 catagagcag gatgtccccc agtccacgct cgatgcagcg ttcgcgcatg cccagacagt
    10861 cgatatcgcc atgcccatag atagaggaga cgacaatcgc gtcagcgcca gtttcaatgg
    10921 cagcatcaat atattcgtcc tggctgacca ttacgcccag attaatgact ctgaagtcgt
    10981 ggttgctaaa aacgcggtcc agaactttat tgcctactgc atggcagtcc gcgccaataa
    11041 cgccaatcac aagtgttgct ttcttcatgg gtaatccttt gtaagacaga gagtaaaata
    11101 agttacacac gtcgtattta tggccgttca taacggcaca ggtttaatat cacaggagtg
    11161 tctttatctg accgtaatgt tgcaattgat ctattttcaa aatgtctttc gggaaatgat
    11221 tgtttaaagt gtgtttttaa atttccatgt taattcagtg tgttatgtca actaatgtca
    11281 tgagtgttaa atttttaaaa ttaacttgat ttaatgtatt gagataattg tgatctactt
    11341 ctaatttgca tttttgtgac gtagttt
//
LOCUS       DAZGBC010000156        11345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGBC010000156 DAZGBC010000000
VERSION     DAZGBC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.157"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(189..2018)
                     /gene="glmS"
                     /locus_tag="ACHK1J_004053"
     CDS             complement(189..2018)
                     /gene="glmS"
                     /locus_tag="ACHK1J_004053"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO7913257.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2180..3550)
                     /gene="glmU"
                     /locus_tag="ACHK1J_004054"
     CDS             complement(2180..3550)
                     /gene="glmU"
                     /locus_tag="ACHK1J_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO7913258.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELQEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3903..4322)
                     /gene="atpC"
                     /locus_tag="ACHK1J_004055"
     CDS             complement(3903..4322)
                     /gene="atpC"
                     /locus_tag="ACHK1J_004055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO7913259.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4343..5725)
                     /gene="atpD"
                     /locus_tag="ACHK1J_004056"
     CDS             complement(4343..5725)
                     /gene="atpD"
                     /locus_tag="ACHK1J_004056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO7913260.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRCLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5752..6615)
                     /gene="atpG"
                     /locus_tag="ACHK1J_004057"
     CDS             complement(5752..6615)
                     /gene="atpG"
                     /locus_tag="ACHK1J_004057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO7913261.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6666..8207)
                     /gene="atpA"
                     /locus_tag="ACHK1J_004058"
     CDS             complement(6666..8207)
                     /gene="atpA"
                     /locus_tag="ACHK1J_004058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO7913262.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8220..8753)
                     /gene="atpH"
                     /locus_tag="ACHK1J_004059"
     CDS             complement(8220..8753)
                     /gene="atpH"
                     /locus_tag="ACHK1J_004059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO7913263.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8768..9238)
                     /gene="atpF"
                     /locus_tag="ACHK1J_004060"
     CDS             complement(8768..9238)
                     /gene="atpF"
                     /locus_tag="ACHK1J_004060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO7913264.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9300..9539)
                     /gene="atpE"
                     /locus_tag="ACHK1J_004061"
     CDS             complement(9300..9539)
                     /gene="atpE"
                     /locus_tag="ACHK1J_004061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO7913265.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9586..10401)
                     /gene="atpB"
                     /locus_tag="ACHK1J_004062"
     CDS             complement(9586..10401)
                     /gene="atpB"
                     /locus_tag="ACHK1J_004062"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO7913266.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10410..10790)
                     /gene="atpI"
                     /locus_tag="ACHK1J_004063"
     CDS             complement(10410..10790)
                     /gene="atpI"
                     /locus_tag="ACHK1J_004063"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO7913267.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2741 a         3049 c         2795 g         2760 t
ORIGIN      
        1 agtacgtcaa ttatggcaat tgtttgtcaa tgattcgagg tatttaatta ctactacggt
       61 taaaacaagt gttcaacggt gccccaaata atagctgaag ttaattatat ggaatgaggt
      121 tagattatta atgctggtaa gtaaaagggc cgccaaaagg cagcccttcg ttcagcaaat
      181 gaataatatt actcaaccgt taccgatttt gccaggttac gcggctggtc aacgtcggtg
      241 cctttgatca gcgcgacgtg ataagccagt aactgcagcg gaacggtgta gaagatcggt
      301 gcaatcacct cttccacatg cggcatctcg atgatgtgca tgttatcgct acttacaaaa
      361 cccgcatcct gatcggcgaa gacatacaat tggccgccac gcgcacgaac ttcttcaatg
      421 ttggatttca gtttttccag caattcgttg ttcggcgcaa caacgataac cggcatatcc
      481 gcgtcaatca gcgccagcgg accgtgtttc agttcacctg cagcgtaggc ttcagcgtga
      541 atgtaagaga tctctttcag cttcaatgcg ccttccagcg cgattgggta ctgatcgcca
      601 cggcccagga acagcgcgtg atgtttgtca gagaaatctt ctgccagagc ttcaatgcgt
      661 ttgtcctgag acagcatctg ctcaatacgg ctcggcagcg cctgcagacc atgcacaatg
      721 tcatgttcaa tggaggcatc cagacctttc aggcgagaca gcttcgccac cagcatcaac
      781 agcacagtta actgggtggt gaatgcttta gtggatgcca cgccgatttc tgtacccgcg
      841 ttggtcatta gcgccagatc ggattcgcgc accagagaag aacccggaac gttacagatt
      901 gccagtgaac caaggtaacc cagctctttc gacagacgca ggcccgccag ggtatctgcg
      961 gtttcgccag actgtgacaa ggtgatcatc aggctgttac gacgcacggc agatttgcga
     1021 tagcggaatt cagaggcgat ttcgacgtcg cacggaatac ctgccagcga ttcaaaccag
     1081 taacgggaaa ccataccgga gttataagaa gtaccacagg cgaggatctg aatatgctca
     1141 accttcgaca gcagttcgtc ggcgttcggt cccagctcgc ttaaatcaac ctgaccgtgg
     1201 ctgatgcgcc cggtaagggt gtttttgatc gcgttcggct gttcgtagat ctctttctgc
     1261 atgtagtgac ggtaaatacc tttatcgccc gcgtcatatt gcagattgga ttcgatatcc
     1321 tgacgtttta cttccgcgcc agttttatcg aagatgttta ccgaacggcg agtaatttcc
     1381 gcaatatcgc cctcttcaag gaagataaag cgacgggtca ccggcagcag cgccagctgg
     1441 tcagaagcga taaagttttc gcccataccc agaccaatca ccagcggact accagaacgt
     1501 gctgccagca gggtatccgg gtgacgggag tccatgatca ctgtaccgta cgcaccacgc
     1561 agttgcggga tagcacgcag aacagcctca cgcagagtac cgccctgttt cagctcccag
     1621 ttcaccagat gggcaatcac ttcggtgtcg gtttcagaaa cgaaggtata gccacgcgct
     1681 tttaactctt cacgcagcgg ttcatggttt tcgatgatgc cgttatgcac caccacaatg
     1741 tgttcagaaa catgcggatg cgcgttaact tctgaaggtt caccatgggt cgcccagcga
     1801 gtgtgagcaa taccggtgcc gccatgcaga ggatgttctt ccgctgcctg agccagcatc
     1861 tggactttac cgaggcgacg caggcgggtc atatgacctt ccgcatcaac aacggccaga
     1921 ccggcagagt catatccgcg gtattccaga cgacgtaaac cttcaagaag gatttctgct
     1981 acatcacgtt gcgcgatcgc gccaacaatt ccacacatag tttttgattc cgatttatat
     2041 cgttgtcggt caacctgtat gcccgttttt tcgggcgccc cgagccttgt agagagtggg
     2101 gttattttta tagttactgc ttgtgggagg gagattatgt tatctcctca tcccatgtga
     2161 ccggattacc cggccagaat cactttttct ttaccggacg acgccagcct tctttctgag
     2221 tctgcggcac acggctgata gctaatgcat tttcgccgac attacgcgtc acagttgtac
     2281 ccgcagcaat ggtcgcgcct ttgcctactg ttaccggggc caccagctga gtgtcggaac
     2341 cgacaaacac atcgtcgccg ataatggtct taaatttatt cgcaccatcg tagttgcagg
     2401 taatggttcc cgcgccgatg ttaacgttat cgccaatttc cgcatcgccc aggtaagtca
     2461 gatgaccagc tttcgagcct ttacccagac gcgctttttt catctcaaca aagttaccga
     2521 cgtgtgcacc ttcctgcaac tcagcaccag gacgcaaacg ggcaaacggg ccaatggtac
     2581 aggccgccgc cagattcgcg tcttccacga cggtatatgg gctaatttcg caatcatcgc
     2641 caatcacgct gtttttaatc acgcaaccgg tgccgatttt cacgcgatga ccgagagtca
     2701 cgttgccctc gatgataacg ttagtatcaa tttcaacatc gcgcccgtga gtaagcgtac
     2761 cacgcagatc aaaacgcgcc ggatcgcgca gcataacgcc tgctaacagc agtttttcag
     2821 cctgttcgga ctgataaaca cgctccagac gggagagttg caggcggtta ttcacgcctt
     2881 ctacttcgct taaacgttgc ggatgaacgg cgacgatttc acgcccttcc tgatacgcca
     2941 gtgcaataat gtcggtgatg tagtattcgc cctgagcatt attgttggtc agcttcgcca
     3001 gccagcgttt catatctgcg ccgttagcga tcagaatgcc ggtgttgatc tcctgaatct
     3061 gacgctgctc gtcggtggca tctttgtgct caacaatgcc ggtaactttg ccgttttcac
     3121 gggtgatacg tccataaccg gtcggatcat ccagtttcac cgtcagcaga ccaatgccac
     3181 cctgcggttt agcatcacgc agacgctgga gtgtttcgac agagatcagc ggcacgtcgc
     3241 cgtagagcat taaaatgtct tcatcatcgg caaagaaagg tgcagcctgc tgcattgcat
     3301 gaccagtacc cagctgttct gcctgaagca cccagttcag gttgtcgtct ttcagcgcct
     3361 gttttagcag atcgccgccg tgaccgtaca ccaggtgaac gtgcgctgcg cctaattcat
     3421 tcgcagcatc aatgacatgc tgaaccatcg ctttcccggc aagggtatgc agcactttcg
     3481 gaagatcgga atacatgcgc gtgcctttac ctgcggcaag gatcactacg ctcatagcat
     3541 tattcaacat acgcgtcctg actgtaattt gagaacgaat ttaaaccgct tcaccttgaa
     3601 aaaactacat ttttttcatc gtgaaatgga cagaggataa attgttcaat aacggattat
     3661 ccccgctgac gaaagcgcca ttttcgacca tcgtcacgcc ttttgtctgc agaaaattaa
     3721 ggcagtacct tcatttaagc gtcaggtgga tgtttttgct cttatttcga tcaatgaata
     3781 aacgggaaaa cagggggttt tatatcagca ggatctatgt gaacgctatt caggacgggt
     3841 cacacgcgca aaaaaaaagc cagcctgttt ccagactggc ttttgtgctt ttcaagccgg
     3901 tgttacatcg cttttttggt caactcgata acgcgcagct gcgcgatcgc tttggccagt
     3961 tccgcagacg cctgagcgta atctacgtcg ccgtgagagc tgctaatgtg ctcttcagcc
     4021 ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct ggccgcgaat tgcggtgtcg
     4081 gccagaacgg tcacgttgcc aggctgcact tcaagaatgc cgccagacag atagataaac
     4141 tcttcgtgac cgtgctgttt cacgatgcga atcataccag gcttaatggc ggtgagcagc
     4201 ggtgcgtggc cagggtagat ccccagttca ccttcgctac ccgttacctg gattttctcg
     4261 accagaccag agaacatttg ttgctctgcg ctgacgacgt ccaggtggta agtcattgcc
     4321 atatcaccct ccgattaagg cgttaaagtt ttttggcttt ttccacagct tcttcgatgg
     4381 aaccgaccat gtagaacgcc tgctccggca ggtgatcgta ttcgccttcc atgatgcctt
     4441 taaagccacg gatggtgtct ttcagggaga cgtatttacc cggagaaccg gtgaatactt
     4501 ctgccacgaa gaacggctgg gacaggaagc gctggatctt acgagcacgt gctaccacca
     4561 gtttgtcttc ttcagacagt tcatccatac ccaggatggc gatgatgtct ttcagttcct
     4621 gataacgttg caggatggac tgaacgccac gcgcggtgtc gtagtgttcc tgaccaacca
     4681 ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc aacggccggg tagataccca
     4741 gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag gtgcgcaaag gtggttgccg
     4801 gagacgggtc agtcaagtca tccgcaggta cgtatactgc ctgtacggag gtgatagaac
     4861 cggttttggt ggaggtgata cgttcctgca gaacgcccat ctcttccgcc agggtcggct
     4921 gataacctac cgctgaaggc atacggccca gcagtgcgga tacttccgta ccggccaggg
     4981 tgtaacgata gatgttgtca acgaacagca gaacgtcacg accttcgtca cggaatttct
     5041 cagccatggt cagaccggtc agagcaacgc gcagacggtt tcccggcggc tcgttcatct
     5101 ggccatacac cagggatact ttgtcgataa cgttggagtc ggtcatttcg tggtagaagt
     5161 cgttaccctc acgagtacgt tcacctacgc ccgcaaacac agagtaaccg gagtgctcga
     5221 tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt acctacaccc gcaccaccga
     5281 acagaccaac tttaccgccc ttagcgaacg gacacatcag gtcgataact ttgataccgg
     5341 tttccagcag ttcctgagag tttgacagct cttcgtagga aggtgctgcg cggtgaatcg
     5401 cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac cggttcaccc agtacgttca
     5461 tgatacggcc cagagtcgct ttacctaccg ggacttcgat cgggtgttcg aggtctttta
     5521 catccagaca gcgacgcaga ccgtcggagg aacccattgc gatggtacgc acgataccgc
     5581 cgccgagctg ctgctgaact tccagcacca gacgctcatt accattttgc acctcaagag
     5641 catcgtacac gcgcggtacg gcatcctgag ggaattcgac gtcaactacg gcgccgatta
     5701 cctggacaat ctttccagta gccatcttaa atcctctacg aaataacctg tttaaaccgc
     5761 ggcggccccc gagacgatct cggtgagttc ctgagtaatg ctggcctgac gagctttgtt
     5821 gtataccaac tgcagctctt taatcaggct gccgccattg tcggtcgcgg ctttcatcgc
     5881 caccatacgg gcggcctgct cgctggccag gttttcaacc acgccctgat aaacctgaga
     5941 ttcgacataa cgacgcagca gggtatccag caacgccttc ggatcgggtt cgtacaggta
     6001 atcccaggat ttatgtttca gatcatcatc atctgatgcc ggtaacggca gcagctggct
     6061 gatggtcgga acctgagaca tggtgttaat aaatttgttg ctgacaatgt aaagcttgtc
     6121 cagacggcct tcgtcgtagg cctgcaacat cacttttacc ggaccgatca gttcggacag
     6181 ggaagggtta tcccccatgc cggtgacctg ggcaacaaca ttgccgccca cggagttgaa
     6241 gaacgacacg cctttcgagc cgatcattgc gaggtcgcat tgaacgcctt tgtcggtcca
     6301 ggtcttcatt tccgccagca gttttttgaa caggttaatg ttcaaaccac cgcacaaacc
     6361 acggtcggtc gacaccacca ggtagcccac gcgtttaacg tcgcggtctt ccaggtaagg
     6421 gtgcttgtat tccagattac cgtgtgcaag gtgaccaatc actttgcgca tggtttctgc
     6481 ataaggacgg ctggccgcca tgcgatcctg cgatttacgc attttggaag cggcgaccat
     6541 ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt gcgatcttac tacgtatctc
     6601 ttttgcgccg gccatgagct tctcctcaat gccttgcggc ctgccctaag gcaagccgcc
     6661 agacgttacc aggattgggt tgctttgaag gaatcgagga tgcctttcag cttgccttcg
     6721 atttcgtcgt tgtagccacc ggtctggttg atctcttgca tcaacggagc gtgatcacgg
     6781 tcgacgtaag ccagcagagc ggcttcgaag ctgccgattt tcgacagttc aacatccgcc
     6841 aggtaaccac gttctgctgc gaacagaacc agagactgtt gcgctacgga catcggcgca
     6901 tactgtttct gtttcagcag ttcggtcact ttctgaccgt ggtcaagttg cttacgtgtt
     6961 gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca gttcacgata ctgtgccaga
     7021 gcggtacgga taccaccgga cagttttttc atgatcttgg tctgtgctgc accaccaaca
     7081 cgggatacgg aaatacccgg gttaaccgca ggacgaatac cggcgttgaa caggttggtt
     7141 tccaggaaga tctgaccatc ggtaatggag attacgttgg tcggaacgaa cgcagaaacg
     7201 tcacccgcct gagtttcgat aatcggcagt gcggtcagag aaccggtttt ccctttcact
     7261 tcacctttgg tgaaggcttc aacgtattcg gcgttaacac gcgcagcacg ctccagcaga
     7321 cgagagtgga ggtagaatac gtcgcccggg aatgcttcac gtcctggcgg acgacggagc
     7381 agcagggaga tctgacggta agcaacagcc tgtttagaca ggtcatcgta aatgatcagc
     7441 gcatcttcac cgcggtcacg gaagtattcg cccattgcgc aaccggcata cggtgccagg
     7501 tattgcagtg cagcggattc agacgcggtt gctaccacaa cgatggtgtt agccagtgcg
     7561 ccgtgctctt ccagtttacg taccacgtta gaaatggtgg acgctttctg gccgatagcg
     7621 acatagatac atttgatacc ggaatcgcgc tggttgatga tggcatcgat agccagtgcg
     7681 gttttaccgg tctgacggtc accgatgatc aattcacgct gaccacgacc gattgggatc
     7741 atggagtcaa cggctttata accggtctgt accggctgat ctacggactg acgttcgata
     7801 acgcccggag cgattgcttc tacagcagag aagccgtcgt gatccagcgg acctttaccg
     7861 tcgattggtg cacccagagt gttaaccaca cggcccagca ggccacggcc aaccggaact
     7921 tccaggatac ggccagtaca cttaactttc atgccttcgg caaggtcagc gtacggaccc
     7981 ataacaaccg cacctacaga gtcgcgctcg aggttcagtg cgatagcgta acggttaccc
     8041 ggcagggaga tcatttcacc ctgcatacaa tcggccaggc cgtgaatgcg gataacaccg
     8101 tcacttacag aaacaatagt accttcgttg tgagcttcac tcacaacatt gaactgagca
     8161 atgcgctgct tgatcagttc gctgatttcg gtggaattca gttgcatgct ccagtcccct
     8221 taagactgca agacgtctgc aaggcgctca agacgaccgc gtacgctgcc atcaatgacc
     8281 atatcacccg ctcggatgat aacgcctgcc attacagact tatcgatttt gcaattcagc
     8341 ttaactttgc gtgacagacg tttttccatc gcagcagaaa ttttcgcgag ctgttgttca
     8401 ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag cctcactcac ggcacgaagg
     8461 tgaataaact gctccagaac atccgggagc gcgttaagac gaccattttc agccattacc
     8521 cgaataaggt tctgaccgtt ttcgtccagt tgctcaccac aaactgcgat aaacgactcg
     8581 gcaagcgttt ctggcgcaag cgcgccagag agaagctctg ccatttgttc gtttttggtt
     8641 acctcggcgg caaacgccag catgtcctgc cagcgttcta cactttggtg ttcgacggca
     8701 aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa taaattcaga catcagcccc
     8761 tccctcctta cagttcagcg acaagtttat ccacgatgtc gctgttagca gcttcatcca
     8821 cggaacgttc gatgatcttc tcggcgccag caacagccag gatagcaact tgcttacgca
     8881 gctcttcacg ggcacgttta cgctcggctt caatttccgc ctgcgcctgg gccacgattt
     8941 tagtacgttc ctgttctgcc tcagctttcg cttcgtccag aatctgcgag cggcgtttgt
     9001 tcgcctgctc gatgattacc tgggcttccg ctttcgcttt tttcagctgg tcggtcgcgc
     9061 tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc ggaagcaagg ccgtcagcaa
     9121 tttctttttg acgtttttcg atggctgcca ttaatggcgg ccatacgtac ttcatgcaga
     9181 acagaacgaa caggacaaac gcgatggcct ggccgaggat tgttgcgtta agattcacag
     9241 cacaatgcct ctatttagtt aacgttctga tattgctctt taaataaaag caacgcttac
     9301 tacgcgacag cgaacatcac gtacagaccc agacctacag cgatcatcgg gatagcatcc
     9361 accagaccca taacgataaa gaactgagta cgcagcagag gaatcagatc aggttgacgc
     9421 gctgcgcctt ccaggaattt acccccgagg atgccgatac cgatcgcagc accgattgcc
     9481 gccagaccca tcatcacagc ggcagccatg tacagcagat ccatattcag gttttccatg
     9541 acagtctcca gtttgtttca gttaaaacgt agtagtgttg gtaaattaat gttcttcaga
     9601 cgccatcgac agatagacga tcgtcagaac catgaagatg aaggcttgca gcgtaatgat
     9661 caggatgtgg aaaatggccc acggcacatt caggatccac tgtgaccacc acggcaacag
     9721 accagcaatc agaatgaaaa tcagctcacc ggcatacatg ttaccgaaca gtcgcaaacc
     9781 gagtgaaact ggtttggaca gcaggcttac cccttcaagg attaagttga caggaatgaa
     9841 cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg aagccgccga tgcctttcat
     9901 tttgatgctg tagaacagaa tcaggataaa tacgcccagt gccatagaca gcgttacgtt
     9961 cacgtccgca gacggaacca cacgcagtgc aggcagaccc agtacatgtt cagcaatgta
    10021 cggcagcagg tcgataggca gtaaatccat caggttcatc aggaataccc agacgaagat
    10081 cgtcagggcc agcggagcaa tcagcttgct tttgccatgg tacatgtctt tcacgctacc
    10141 attaacaaag ccgatcacca gctcaatcgc ggtctgaaac ttacctggca caccgctggt
    10201 cgcctttttg gctacgctac ggaataaaac caggaacaac agacccagca ccaccgagaa
    10261 gaacatggag tcaatattga ttgtccagaa ggtggctggg gggttttgtg gatccaccag
    10321 cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg tgtcctatgt aatcctgcgg
    10381 cgtcatattt tctgaagcca tgatgccttt taccctttgt tgttaattac agccggtgcc
    10441 agtatctgaa ccaccagcac caaaacccac gtaacgatca gcggcaagaa taccgccttt
    10501 aaaaccgcca acgccaccac cagtaacacc aacatcgcca gaactttgaa agcttcgcca
    10561 aatgcgaatg tccaggccac ccggcctttc gctggtgtat gcgcctggtg acgccaggca
    10621 aatatcataa acaaaacgtt aggcagaaag actgccaggc ccccgcttat tgcagagacg
    10681 ccccagaagg ggtctttgag gctgaacagc aatccacttg ctatcaccac cagtaactga
    10741 acgagcagaa gcttccgagc aacgtttcga ctcacgagcg acacagacat cacgtttttc
    10801 actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa ctttctttaa ggcttagagt
    10861 caagcatcaa aaagcggtca aattatacgg tgcgcccccg tgatttcaaa caataagtag
    10921 ccaaaaggtg aataaatgtt taaatatttt tccagtgcat acaattgcga cttttctgct
    10981 aaccctgttt gatcatgaaa aactgtaaat aacgcgtaaa cactggtgat aaagcgtgct
    11041 tcagatcaca tattgcgcat gttcgcgcac agcatattta tttacttggc aaatgatgcc
    11101 tttgcaagtt tatgatattt cagtctaaaa acagatactg ttttaataaa tgacatttac
    11161 acaacaaaaa ccacccattg acatttttaa taatgtttta acagacaatg atggttctta
    11221 gcgccgattt ttagcagact gatattttca ctaatgactt attttctgct taccaaaaaa
    11281 agccacgtta tcttgttgat gcaaaagagt gaacgtggcg ttaaatgtaa ccagttatat
    11341 cagta
//
LOCUS       DAZGBC010000157        11232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGBC010000157 DAZGBC010000000
VERSION     DAZGBC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.158"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..248
                     /gene="yjeJ"
                     /locus_tag="ACHK1J_004064"
     CDS             <1..248
                     /gene="yjeJ"
                     /locus_tag="ACHK1J_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7913268.1"
                     /translation="EQFSGAVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVPCQVY
                     VRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(441..794)
                     /locus_tag="ACHK1J_004065"
     CDS             complement(441..794)
                     /locus_tag="ACHK1J_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO7913269.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(932..2578)
                     /gene="groL"
                     /locus_tag="ACHK1J_004066"
     CDS             complement(932..2578)
                     /gene="groL"
                     /locus_tag="ACHK1J_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO7913270.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2622..2915)
                     /locus_tag="ACHK1J_004067"
     CDS             complement(2622..2915)
                     /locus_tag="ACHK1J_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO7913271.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3191..4447
                     /gene="yjeH"
                     /locus_tag="ACHK1J_004068"
     CDS             3191..4447
                     /gene="yjeH"
                     /locus_tag="ACHK1J_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO7913272.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4463..4939)
                     /locus_tag="ACHK1J_004069"
     CDS             complement(4463..4939)
                     /locus_tag="ACHK1J_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO7913273.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5276..6712
                     /gene="aspA"
                     /locus_tag="ACHK1J_004070"
     CDS             5276..6712
                     /gene="aspA"
                     /locus_tag="ACHK1J_004070"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO7913274.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6830..8131
                     /gene="dcuA"
                     /locus_tag="ACHK1J_004071"
     CDS             6830..8131
                     /gene="dcuA"
                     /locus_tag="ACHK1J_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO7913275.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8247..8585
                     /gene="cutA"
                     /locus_tag="ACHK1J_004072"
     CDS             8247..8585
                     /gene="cutA"
                     /locus_tag="ACHK1J_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO7913276.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8561..10258
                     /gene="dsbD"
                     /locus_tag="ACHK1J_004073"
     CDS             8561..10258
                     /gene="dsbD"
                     /locus_tag="ACHK1J_004073"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO7913277.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10295..10870
                     /locus_tag="ACHK1J_004074"
     CDS             10295..10870
                     /locus_tag="ACHK1J_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7913278.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10977..11052
                     /locus_tag="ACHK1J_004075"
     tRNA            10977..11052
                     /locus_tag="ACHK1J_004075"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11010..11012,aa:Phe,seq:gaa)
BASE COUNT         2635 a         2949 c         2803 g         2845 t
ORIGIN      
        1 aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc aacgccgggt aaagaaatag
       61 aggcgatcac tcgccgaatg cttgatttca gcccacgact gaaaaaactc gcgggtgtgc
      121 catgtcaggt ctatgttcgc accgtagcag caaataacgc ccaaccgcta acccaggatc
      181 aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact gcaccacagg
      241 cgaaataatc tcatctttgc taatgcgagg ctggagccat ccagcctgaa atgactgata
      301 acgtgatcgt tggtcatgcc ggatgcggcg taaacgcctt atccggccta caaattctca
      361 agcattcaat cagttgcaat gcattatgta ggcctgataa gcgaagcgca tctggcaatt
      421 atacgtttgt caccatcaca tcagttcggg cacttatata cctggccgat aatctggcta
      481 tccgtcggga caaaactgga caacattccc tgcgacgggc tactgatgcc ataaatcacg
      541 ttaccgccca ttgcagccgc ctggttgcgc agatcgtttg ctgcgccgcg catagaaccg
      601 ccctcttctc cgtgttgccc ggaaagccag ttgctttgct tacctgtcgc agtaccaatc
      661 agctggcact ctgcgcctgg ctgctcgtcc acaatgcgta cactctgacc ggcagcactc
      721 aattcgttgc tggagctaca acccgccatc agtagcgcgg caccgacaat ccctgctaag
      781 tattttacgt gcatgttatt ccccataatc aatgagctgg acgtcactcg cgtcgtccgt
      841 gtctgaatct tatactagaa agatgaccaa aagaaaaacc cccagacatt tctgcccggg
      901 ggtttgttta tttctgcgag gtgcagggca attacatcat gccgcccatg ccacccatgc
      961 cgcccatacc gccagcagcg cctaagtcag ctgcatcgtt tttcggcagg tcggtaacca
     1021 tgcactcggt ggtgatcatc aggccagcca cagaagccgc gtactgcaga gcagaacggg
     1081 ttactttggt tggatccagg atacccatgt cgatcatgtt gccgtattct tcggttgctg
     1141 cgttgtaacc gtagttgccg tcgccgcctt taacggtgtt agcaacaaca gacggttctt
     1201 cgccgcagtt caggacgatc tgacgcagcg gagcttccat tgcacgcagt gcaactttga
     1261 tacccacgtt ctggtcttcg ttctgaccac gcaggtcagc cagtttagac gctacgcgga
     1321 tcagcgcaac accaccacca gcaaccacgc cttcttctac cgcagcacgg gtcgcgtgca
     1381 gggcgtcttc aacgcgtgct tttttctctt tcatttcaac ttcggtagca gcacctactt
     1441 tgataactgc aacgccgcct gccagtttcg ctacgcgctc ctgcagtttt tcacggtcgt
     1501 agtcagaagt tgcttcttca atctgctgac ggatctgagc aacacggccc tggattgcag
     1561 cttcttcacc cacgccatcg atgatagtgg tggtgtcttt gttgatcaca acacgtttag
     1621 cctgacccag gtcttccagg gttgcttttt ccagctccat accgatctct tcagagatta
     1681 cggtaccgcc agtcagggtt gcgatatcct gcagcatagc tttacgacga tcgccgaagc
     1741 ccggagcttt aactgcagca actttcacga tgccacgcat ggtgttaaca accagagttg
     1801 ccagcgcttc gccttctaca tcttcagcga tgatcagcag cggtttgcct gctttggcaa
     1861 cggcttccag aaccggcagc atttcgcgga tgttggagat tttcttgtca gccagcagga
     1921 tgaacgggct ttccagttct actgcgccag tttccggctt gttgatgaag taaggagaca
     1981 ggtagccacg gtcgaactgc ataccttcaa ccacgtccag ttcgtcctgc agaccggtac
     2041 cgtcttcaac ggtgataacg ccttctttac cgactttgtc catcgcttca gcgatcagtt
     2101 tacctacggt ttcgtcggag ttagcggaga tagtaccaac ctgagcaatc gctttagagt
     2161 cagagcacgg tacggacagc gctttcagtt cttcaactgc agcggtaaca gctttgtcga
     2221 taccacgttt caggtccatc gggttcatgc ccgcagcaac agctttcaga ccttcagtga
     2281 tgatagcctg agccagtacg gttgcagtgg tggtaccgtc gcctgcagcg tcgttcgctt
     2341 tagaggcaac ttctttcacc atctgcgcac ccatgttttc gaacttgtct tccagttcga
     2401 tttcacgagc aacggaaaca ccatctttgg tgatggtcgg tgcaccgaaa gatttatcca
     2461 gaactacgtt acggcctttc ggaccgaggg taactttcac tgcatctgcc agtacgttta
     2521 cgccgcgcag cattttcaca cgagcgtcgt taccgaattt tacgtcttta gctgccatta
     2581 tctttattcc ttaaattcgt atgttcagtg tcgtgcgagg attacgcttc aacaattgcc
     2641 agaatgtcgc tttcggacat gatcaacact tcttcattgt cgatcttctc ggatttcaca
     2701 ccgtagccat cgttgaaaat aacgatgtcg ccaactttca catccagcgg cttcacttcg
     2761 ccattttcaa ggatacggcc attgccgaca gccagcactt cgccgcgggt ggatttagcc
     2821 gctgcagagc cggtcagaac gatgccgcca gcagatttag tttcaacttc tttacgcttg
     2881 acgatcacgc gatcatgcaa tggacgaata ttcattgata actctccttt gagaaagtcc
     2941 gtatctgtta tgggtgacgc cggagcttac gtggtttccc ggctggtgac cagagaaatg
     3001 gggatgaggc ttcgcccctt caagggggaa aaagaaaaaa aattctgatc agcacaaaat
     3061 cgggtgaaaa ccctgattca cctcacattt catcgcaatt tcttcatcgc cgtcataagc
     3121 gaatctgatt gtgctaccat cgaaaatcta cgcagttgcc caaaatttgg gcgcaatcgg
     3181 acatcaaccc atgagtggac tcaaacaaga actggggctg gcccagggca ttggcctgct
     3241 atcgacgtca ttattaggca ctggcgtgtt tgccgttcct gcgttagctg cactggtagc
     3301 gggcaataac agcctgtggg cgtggcccgt tttgattatc ttagtgttcc cgattgcgat
     3361 tgtgtttgcg attctgggtc gccactatcc cagcgcaggc ggcgtcgcgc acttcgtcgg
     3421 tatggcgttt ggttcgcggc ttgagcgagt caccggctgg ctgtttttat cggtcattcc
     3481 cgtgggtttg cctgccgcgc tacaaattgc tgccggattc ggccaggcaa tgtttggctg
     3541 gcatagctgg caactgttgt tggcagaact cggtacgctg gcgttggtgt ggtatatcgg
     3601 tactcgcggt gccagttcca gtgcaaatct acaaaccgtt attgccggac ttatcgtcgc
     3661 actgattgtc gctatctggt gggcgggcga tatcaaacct gcgagtatcc ccttccccgc
     3721 gccaggaaat atcgaactta ccgggttatt tgctgcgtta tcagtgatgt tctggtgttt
     3781 tgtcggtctg gaagcatttg cccatcttgc ctcggaattt aaaaatccag aacgtgattt
     3841 tcctcgtgct ttgatgattg gtctgctgct ggcaggatta gtctactggg gctgtacggt
     3901 agtcgtctta cacttcgacg cctatggtga acaaatggcc gcggcagcat cgcttccaaa
     3961 aattgtagta caactgttcg gtgtaggagc gttatggatt gcctgcgtaa ttggctatct
     4021 ggcctgcttt gccagtctca acatttatat acagagcttc gcccgcctgg tctggtcgca
     4081 ggcgcaacat aatcctgacc actacctggc acgcctctct tctcgccata tcccgaataa
     4141 tgccctcaat gcggtgctcg gctgctgcgt ggtgagcacg ttggtgattc atgctttaga
     4201 gatcaatctg gacgctctta ttatttatgc caatggcatc tttattatga tttatctgtt
     4261 atgcatgctg gcaggctgta aattattgca aggacgttat cgactactgg cggtggttgg
     4321 cgggctgtta tgcgttctgt tactggcaat ggtcggctgg aaaagtctct atgcgctgat
     4381 catgctggcg gggttatggc tgtttctgcc aaaacgaaaa acgccggaaa atggcataac
     4441 cacataatcc ggcgtttcga cattaatcct ggcgatcgtc tttatgatca aggcggtcgc
     4501 gctcatcatc ctttcgctgg tactcaccat caaaagtatt accgccaccg gtcccggcgc
     4561 taaaaccgcc gcctggcatg cgagaaaagc gcaaatgtgg catcaacttc actgtcagat
     4621 gcttttgcac cggcggcaat aaaagcagaa gaccgaggaa gtcggtgaaa aagcccggta
     4681 ataaaagcag caaaccggca atgatcagcg aaacactttt aatcatctcc gccgctgggt
     4741 tttcacccgc cgccattttt tgctgcatca gcacgaaatt cttaaagccc tggttacgta
     4801 ccagtgacat accgataact gacgtgaata taaccagcac gagggtcaac aataccccca
     4861 atacatgggc aacctgaata aagattgaaa tctcaatata gacataaagg aaaatggcaa
     4921 taaaaggtaa ccagcgcaaa ggtttctcct gtaatagcag ccggttaaac ccggctacct
     4981 gaatgggttg cgaatcgcgt tcagcttata ttgtggtcat tagcaaaatt tcaagatgtt
     5041 tgcgcaacta tttttggtag taatcccaaa gcggtgatct atttcacaaa ttaataatta
     5101 aggggtaaaa accgacactt aaagtgatcc agattacggt agaaatcctc aagcagcata
     5161 tgatctcggg tattcggtcg atgcagggga taatcgtcgg tcgaaaaaca ttcgaaacca
     5221 catatattct gtgtgtttaa agcaaatcat tggcagcttg aaaaagaagg ttcacatgtc
     5281 aaacaacatt cgtatcgaag aagatctgtt gggtaccagg gaagttccag ctgatgccta
     5341 ctatggtgtt cacactctga gagcgattga aaacttctat atcagcaaca acaaaatcag
     5401 tgatattcct gaatttgttc gcggtatggt aatggttaaa aaagccgcag ctatggcaaa
     5461 caaagagctg caaaccattc ctaaaagtgt agcgaatgcc atcattgccg catgtgatga
     5521 agtcctgaac aacggaaaat gcatggatca gttcccggta gacgtctacc agggcggcgc
     5581 aggtacttcc gtaaacatga acaccaacga agtgctggcc aatatcggtc tggaactgat
     5641 gggtcaccag aaaggtgaat atcagtacct gaacccgaac gaccatgtta acaaatgtca
     5701 gtccactaac gacgcctacc cgaccggttt ccgtatcgca gtttactctt ccctgattaa
     5761 gctggtagat gcgattaacc aactgcgtga aggctttgaa cgtaaagctg tcgaattcca
     5821 ggacatcctg aaaatgggtc gtacccagct gcaggacgca gtaccgatga ccctcggtca
     5881 ggaattccgc gctttcagca tcctgctgaa agaagaagtg aaaaacatcc aacgtaccgc
     5941 tgaactgctg ctggaagtta accttggcgc aacagcaatc ggtactggtc tgaacacgcc
     6001 gaaagagtac tctccgctgg cagtgaaaaa actggctgaa gttactggct tcccatgcgt
     6061 accggctgaa gacctgatcg aagcgacctc tgactgcggc gcttatgtta tggttcacgg
     6121 cgcgctgaaa cgcctggctg tgaagatgtc caaaatctgt aacgacctgc gcttgctctc
     6181 ttctggtcca cgtgccggcc tgaacgagat caacctgccg gaactgcagg cgggctcttc
     6241 catcatgcca gctaaagtaa acccggttgt tccggaagtg gttaaccagg tatgcttcaa
     6301 agtcatcggt aacgacacca ctgttaccat ggcagcagaa gcaggtcagc tgcagttgaa
     6361 cgttatggag ccggtcattg gccaggccat gttcgaatcc gttcacattc tgaccaacgc
     6421 ttgctacaac ctgctggaaa aatgcattaa cggcatcact gctaacaaag aagtgtgcga
     6481 aggttacgtt tacaactcta tcggtatcgt tacttacctg aacccgttca tcggtcacca
     6541 caacggtgac atcgtgggta aaatctgtgc cgaaaccggt aagagtgtac gtgaagtcgt
     6601 tctggaacgc ggtctgttga ctgaagcgga acttgacgat attttctccg tacagaatct
     6661 gatgcacccg gcttacaaag caaaacgcta tactgatgaa agcgaacagt aatcgtacag
     6721 ggtagtacaa ataaaaaagg cacgtcagat gacgtgcctt ttttcttgtg agcagtaact
     6781 taaaaataac aatctaatat caacttgtta aaaaacaagg aaggctaata tgctagttgt
     6841 agaactcatc atagttttgc tggcgatctt cttgggcgcc agattggggg gaataggtat
     6901 tggttttgca ggcggattgg gggtgctggt tcttgccgct attggcgtta aacccggtaa
     6961 catcccattc gatgtcattt ccattatcat ggcggttatc gccgctattt ctgccatgca
     7021 ggttgctggc ggtctggact atctggttca tcagacagaa aagctgctgc gccgtaaccc
     7081 gaaatacatc acgatcctcg caccgatcgt gacctatttc ctgactatct ttgctggtac
     7141 tggcaacatc tctctggcga cactgccagt tatcgctgaa gttgcgaagg aacaaggcgt
     7201 taaaccttgc cgtccgctgt ctactgcagt ggtatccgcg cagattgcga tcaccgcatc
     7261 gccaatctca gcggcagtgg tttacatgtc ttccgtgatg gaaggtcatg gcatcagcta
     7321 cctccatctg ctctccgtgg tcatcccgtc caccctgctg gcggttctgg tgatgtcctt
     7381 cctggtcact atgctgttca actccaaact ctctgacgat ccgatttatc gcaagcgtct
     7441 ggaagagggc ctggttgaac tgcgcggtga aaagcagatt gaaatcaaat ccggtgcaaa
     7501 aacgtccgtc tggctgttcc tgctgggcgt agttggcgtg gttatctatg caatcatcaa
     7561 cagcccaagc atgggtctgg ttgaaaaacc gctgatgaac accaccaacg caatcctgat
     7621 catcatgctc agcgttgcaa ctctgaccac cgttatctgt aaagtcgata ccgacaacat
     7681 cctcaactcc agcaccttca aagcaggtat gagcgcctgt atttgtatcc tgggtgttgc
     7741 gtggctgggc gatactttcg tttccaacaa catcgactgg atcaaagata ccgctggtga
     7801 agtgattcag ggtcatccgt ggctgctggc cgtcatcttc ttctttgctt ctgctctgct
     7861 gtactctcag gctgcaaccg caaaagcact gatgccgatg gctctggcac tgaacgtttc
     7921 tccgctgacc gctgttgctt ctttcgctgc ggtgtctggt ctgttcattc tgccgaccta
     7981 cccgacgctg gttgctgcgg tacagatgga tgacacgggt actacccgta tcggtaaatt
     8041 cgtcttcaac catccgttct tcatcccggg tactctgggt gttgccctgg ccgtttgctt
     8101 cggcttcgtg ctgggtagct tcatgctgta atgaccaatc gcggggcgtt cacgccccgc
     8161 tttctttccc gccgactaac atccttcccc cgtccgttgt atagtgacct ctctcttgcg
     8221 gttccatctg ttcttgcgag gtgtttatgc ttgatgaaaa aagttcgaat accgcgtctg
     8281 tcgtggtgct atgtacggca ccagatgaag cgacagccca ggatttagcc gccaaagtgc
     8341 tggcggaaaa actggcggcc tgcgcgacct tgatccccgg cgctacctct ctctattact
     8401 gggaaggtaa gctggagcaa gaatacgaag tgcagatgat tttaaaaact accgtatctc
     8461 accagcaggc actgctggaa tgcctgaagt ctcatcatcc atatcaaacc ccggaacttc
     8521 tggttttacc tgttacacac ggagacacag attacctctc atggctcaac gcatctttac
     8581 gctgatcctg ctactttgca gcacttccgt ttttgccgga ttattcgacg cgccgggacg
     8641 ttcacaattt gtccccgcgg atcaagcctt tgcttttgat tttcagcaaa accaacatga
     8701 ccttaatctg acctggcaga tcaaagacgg ttactacctc taccgtaaac agatccgcat
     8761 tacgccggaa cacgcgaaaa ttgccgacgt gcagctgccg caaggcgtct ggcatgaaga
     8821 tgagttttac ggcaaaagcg agatttaccg cgatcggctg acgcttcccg taaccataaa
     8881 ccaggcgagt gcgggagcga cgttaactgt cacctaccag ggctgtgctg atgccggttt
     8941 ctgttatccg ccagaaacca aaaccgttcc gttaagcgaa gtggtcgcca acaacgcagc
     9001 gtcacagcct gtgtctgttt cgcagcaaga gcagcacacc gcgcaattgc ccttttccgc
     9061 gctctgggcg ttgttgatcg gtattggtat cgcctttacg ccatgcgtgc tgccaatgta
     9121 cccactgatt tctggcatcg tgctgggcgg taaacagcgg ctctccactg ccagagcatt
     9181 gttgctgacc tttatttatg tgcaggggat ggcgctgacc tacacggcgc tgggtctggt
     9241 ggttgccgcc gcagggttac agttccaggc ggcgctacag cacccatacg tgctcattgg
     9301 cctcgccatc gtctttacct tgctggcgat gtcaatgttt ggcttgttta ctctacaact
     9361 cccctcctcg ctgcaaacgc gcctcacgct gatgagcaat cgccaacagg gcggctcacc
     9421 cggcggagtg tttattatgg gggcgattgc cggactgatc tgttcaccat gcaccaccgc
     9481 accgcttagc gcgattctgc tgtatatcgc ccaaagcggg aacatgtggc tgggcggcgg
     9541 cacgctttat ctctatgcgt tgggcatggg cctgccgctg atgctaatta ccgtctttgg
     9601 taaccgcttg ctgccgaaaa gcggcccgtg gatggaacaa gtcaaaaccg cgtttggttt
     9661 tgtgatcctc gcactgccgg tcttcctgct ggagcgagtg attggtgata tatggggatt
     9721 acgcttgtgg tcggcgctgg gtgtcgcatt ctttggctgg gcctttatca ccagcctaca
     9781 ggccaaacgc ggctggatgc gcgtggtgca aataatcctg ctggcagcgg cattggttag
     9841 cgtgcgccca cttcaggatt gggcatttgg tgcgacgcat accgcgcaaa ctcagacgca
     9901 tctcaacttt acacaaatca aaacggtaga tgagttaaat caggcgctcg ttgaagccaa
     9961 aggcaaaccg gtgatgttag atctttatgc cgactggtgc gtcgcctgta aagagtttga
    10021 gaaatacacc ttcagcgacc cgcaggtgca aaaagcgtta gcagacacgg tcttacttca
    10081 ggccaacgtc acggccaacg acgcacaaga tgtggcgctg ttaaagcatc ttaatgtcct
    10141 tggcctaccg acaattctct tttttgacgg acaaggccag gagcatccac aagcacgcgt
    10201 cacgggcttt atggatgctg aaaccttcag cgcacatttg cgcgatcgcc aaccgtgaac
    10261 gacacttgca gtgggaaaga cggaggaaaa tagcgtgcaa cgtgaagatg tactgggaga
    10321 agccctgaaa ttattagaat tacaagggat tgccaacacc acgctggaga tggttgctga
    10381 acgtgtggat tatccactgg acgagctgcg ccgcttctgg ccagacaaag aggcaatcct
    10441 ctacgatgcg ctgcgttatc ttagccaaca gatagatgtc tggcgtcgtc agctgatgct
    10501 ggacgaaacg caaaccgccg aacaaaagct gctggcacgt tatcaggcgt tatcggagtg
    10561 cgttaaaaac aaccgctatc cgggctgtct atttatcgct gcctgtacgt tttatcccga
    10621 tcctggccac cctattcatc aactggccga tcagcaaaaa agcgcagcct acgatttcac
    10681 ccacgaactg ttaaccacgc tggaagttga cgatccggcg atggtagcaa agcagatgga
    10741 actggtgctg gaaggctgtt taagccgaat gctagtgaat cgtagccagg cggatgtcga
    10801 cactgcacat cggctggcgg aagatatcct gcgcttcgcc cgctgccgcc agggtggcgc
    10861 attgacctga cagaaacaca gaaaagaagc gatttgccgc aatcttaagc agttgaatcg
    10921 cttttactga aattaggttg acgagatgtg cagattacgg tttaatgcgc cccgttgccc
    10981 ggatagctca gtcggtagag caggggattg aaaatccccg tgtcggtggt tcgattccgc
    11041 ctccgggcac catataccag aacacgctgg ttaagggatg agatccttga cgggcggtta
    11101 atacaggatg cggcatcaaa gggtctccgt ttgtgtaagt tgtggttaca ctgattgagt
    11161 tcggtatcgt atcctcaaac agggtgtctt ttgcagagac gccctgttct cgttaacact
    11221 gtatgcttac at
//
LOCUS       DAZGBC010000158        11002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGBC010000158 DAZGBC010000000
VERSION     DAZGBC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.159"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            272..1414
                     /gene="yghO"
                     /locus_tag="ACHK1J_004076"
     CDS             272..1414
                     /gene="yghO"
                     /locus_tag="ACHK1J_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO7913279.1"
                     /translation="MERDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1421..2185)
                     /gene="glcC"
                     /locus_tag="ACHK1J_004077"
     CDS             complement(1421..2185)
                     /gene="glcC"
                     /locus_tag="ACHK1J_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO7913280.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLSASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2436..3935
                     /gene="glcD"
                     /locus_tag="ACHK1J_004078"
     CDS             2436..3935
                     /gene="glcD"
                     /locus_tag="ACHK1J_004078"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO7913281.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHVGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSGEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3935..4987
                     /gene="glcE"
                     /locus_tag="ACHK1J_004079"
     CDS             3935..4987
                     /gene="glcE"
                     /locus_tag="ACHK1J_004079"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO7913282.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4998..6221
                     /gene="glcF"
                     /locus_tag="ACHK1J_004080"
     CDS             4998..6221
                     /gene="glcF"
                     /locus_tag="ACHK1J_004080"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO7913283.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKSDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6226..6630
                     /locus_tag="ACHK1J_004081"
     CDS             6226..6630
                     /locus_tag="ACHK1J_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO7913284.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6652..8823
                     /gene="glcB"
                     /locus_tag="ACHK1J_004082"
     CDS             6652..8823
                     /gene="glcB"
                     /locus_tag="ACHK1J_004082"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO7913285.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMASGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9178..10860
                     /gene="glcA"
                     /locus_tag="ACHK1J_004083"
     CDS             9178..10860
                     /gene="glcA"
                     /locus_tag="ACHK1J_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO7913286.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2578 a         2759 c         3068 g         2597 t
ORIGIN      
        1 tggtgtgata ttgaacattg attaaaacaa tgctttaaat cttaactctg tttattgtga
       61 ataatattta atttatgaat atattaagat aatgccctgg tgggtcgcat taaatccaca
      121 atagtcatgt tgatcggcta acggtggcgc tggtgtatcg taattattca taaatattat
      181 tttatttttc cttatattca ttgcgtggat agttaaggta ccgtccgttg ctgtttattt
      241 tcagcattaa ttatttgtta tccactaagc tatggaacgc gatctgctga tgattaaaat
      301 tgaaaaagta attaataaaa atgaccttaa ggcatttatc gcttttccgt catcacttta
      361 tcccgacgat ccaaactgga tacccccttt attcattgaa cgcaacgagc atttgtctgc
      421 gaaaaatccg gggacggacc atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca
      481 gatagttggg cgtattaccg cgcaaataga taccttgcac cgcgagcgtt acggcaaaga
      541 taccggtcat ttcggcatga ttgacgccat tgatgatccg caggtttttg ccgcgttgtt
      601 tggcgcagcg gaagcgtggt tgaagtcaca aggtgcaagt aagatcagcg gtccattcag
      661 cctgaatatc aatcaggaaa gcggattact gattgaaggt ttcgatacac caccctgtgc
      721 gatgatgcca cacggcaaac cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa
      781 aggcattgat ttactggcgt ggtggatgca gcgaaccgat ctcactttct ctccggcgct
      841 aaaaaaactg atggatcagg tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg
      901 gtttgccgaa gagatgcaga tcctgcgtga gattttcaac tctggctggc agcacaactg
      961 gggatttgtg ccgtttaccg aacatgaatt cgcgaccatg ggcgaccaac ttaaatatct
     1021 ggtgccggac gatatgatct acatcgctga gattgattct gcaccctgcg cgtttattgt
     1081 cggcttgccg aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg
     1141 ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct
     1201 gatgggcgta cgcgacgagt atcagttcag ccgcatcggc ccggtgattg cgctgttatt
     1261 gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg
     1321 gatcctcgaa accaatacag gcatgaataa catgctggaa cgcattgggg cggagccgta
     1381 caagcgttac cgattgtatg aaaagcagat ttaataccag ctaactcagg ttcatctcca
     1441 gcggcaccgc cgagcgaatc aaatggtggc cttccagctc gatatcgtgg agattctttt
     1501 tcacggtccg cacatgatcg cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca
     1561 ccgcgttgta gatccgcgca tgctggcgat cgatctgctt tttttgctgt ggtcgatggt
     1621 agagattatt tacgctggca aacactgaat taaacatcag atcggtcaat gattgcagcg
     1681 taaacaccag cacctgattg tgagaagcct gacaaatggc gagatggaaa gcgtgatcca
     1741 actgcgcatg ttcgatcagc gaaatctctt tgttgttctc actggcggag agcatttttt
     1801 cataacagcg agttatcaca acaaaatcag cctgcgttcc cagcgttgcc gccagccttg
     1861 ccgattcgcc ctccagtaat gcgcgaacat cgagcagatc gtacagcgtt cgcggttgcg
     1921 tactgaacag atggatcagc ggactggtgt cctgcacccg attaagccgt gcgacacgag
     1981 aatcgcgacc ctgcgccgtt tcaataatcc cgcgcccgcg cagcacggtc agcccttcac
     2041 gcagtgcgga gcgtgagaag ccgagctttt cacacagtcg acgttccgag ggaagcggct
     2101 gaccgacctt cagtacgccg tcgataatta accgttcgat actctctgca accacttcgc
     2161 aaatagggcg acgttcatct ttcattcccg gacctcgtgc acaggtagga ccaatttttc
     2221 tgttcttaac tcgcaaaaca cgcacatcac gtaagtgatt atgttacatc aatttaacat
     2281 taagttaacc aaggcaaggt cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag
     2341 agatagacga aaacgaaaag cccgcttaat aactgttcac agaagcagcg cgcaaaaatc
     2401 agctgccaca caacataaca aagcgaagcc tactcatgag catcttgtac gaagagcgtc
     2461 ttgatggcgc tttacccgat gtcgaccgca catcggtact gatggcactg cgtgagcatg
     2521 tccctggact ggagatcctg cataccgatg aggagatcat tccttacgag tgtgacgggt
     2581 tgagcgcgta tcgcacgcgt ccattactgg ttgttctgcc taagcagatg gaacaggtga
     2641 cagcgattct ggctgtctgc catcgcctgc gtgtaccggt ggtgactcgt ggtgcaggca
     2701 ccgggctttc tggtggcgcg ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct
     2761 ttaaagagat cctcgacatt aaccccgttg gtcgccgcgc gcgcgtgcag ccaggcgtgc
     2821 gtaacctggc gatctcccag gccgttgcac cgcataatct ctactacgca ccggaccctt
     2881 cctcacaaat cgcctgttcc attggcggca acgtggcaga aaatgccggc ggcgtccact
     2941 gcctgaaata tggtctgacc gtacataacc tgctgaaaat tgaagtgcaa acgctggatg
     3001 gcgaggcact gacgctggga tcggatgcgc tggattcgcc tggttttgac ctgctggcgt
     3061 tgttcaccgg atcggaaggt atgctcggcg tgaccaccga agtgacggta aaactgctgc
     3121 cgaagccgcc cgtggcgcgg gttctgttag caagctttga ctcggtagaa aaagccggac
     3181 ttgcagttgg tgacatcatc gccaatggca ttatccccgg cgggctggag atgatggata
     3241 acctgtcgat ccgcgcggcg gaagatttta ttcatgtcgg ttatcccgtc gacgccgaag
     3301 cgattttgtt atgcgagctg gacggcgtgg agtctgacgt acaggaagac tgcgagcggg
     3361 tcaacgacat cttgttgaaa gcgggcgcga ctgacgtccg tctggcacag gacgaagcag
     3421 agcgcgtacg tttctgggcc ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc
     3481 cggattacta ctgcatggat ggcaccatcc cgcgtcgcgc cctgcctggc gtactggaag
     3541 gcattgcccg tttatcgcag caatatgatt tacgtgttgc caacgtcttt catgccggag
     3601 atggcaacat gcacccgtta atccttttcg atgccaacga acccggtgaa tttgcccgcg
     3661 cggaagagct gggcgggaag atcctcgaac tctgcgttga agttggcggc agcatcagtg
     3721 gggaacatgg catcgggcga gaaaaaatca atcaaatgtg cgcccagttc aacagcggtg
     3781 aaatcacgac cttccatgcg gtcaaggcgg cgtttgaccc cgatggtttg ctgaaccctg
     3841 ggaaaaacat tcccacgcta caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg
     3901 gtcatttacc tttccctgaa ctggagcgtt tctgatgcta cgcgagtgtg attacagcca
     3961 ggcgctgctg gagcaggtga atcaggcaat tagcgataaa acgccgctgg tgattcaggg
     4021 cagcaatagc aaagcctttt taggtcgccc tgtcaccggg caaacgctgg atgtgcgttg
     4081 tcatcgcggc attgttaatt acgacccgac cgagctggtg ataaccgctc gtgccggaac
     4141 gccgctggtg gcgattgaag cggcgctgga gagcgccggg caaatgcttc cctgcgagcc
     4201 gccgcattat ggtgaagaag ccacctgggg cgggatggtc gcctgcgggc tggcggggcc
     4261 gcgtcgcccg tggagcggtt cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg
     4321 cgctggaaaa catctgcgtt ttggtggcga agtgatgaaa aacgttgccg gatacgatct
     4381 ctcacggtta atggccggaa gctacggttg tcttggcgtg ctcactgaaa tctcaatgaa
     4441 agtgttaccg cgaccgcgcg cctccctgag cctgcgtcgg gaaatcagcc tgcaagaagc
     4501 catgagtgaa atcgccgagt ggcaactcca gccattaccc attagtggct tatgttactt
     4561 cgacaatgcg ttgtggatcc gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga
     4621 actgctgggt ggcgaagagg ttgcgggtca gttctggcag caattgcgtg aacaacaact
     4681 gccgttcttc tcgttaccag gtaccttatg gcgcatttca ttacccagcg atgcgccgat
     4741 gatggattta cccggcgagc aactgatcga ctggggcggg gcgttacgct ggctgaaatc
     4801 gacagccgag gacaatcaaa tccatcgcat cgcccgcaac gctggcggtc atgcgacccg
     4861 ctttagtgcc ggagatggtg gctttgcccc gctatcggct cctttattcc gctatcacca
     4921 gcagcttaaa cagcagctcg acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga
     4981 actttgagga gcaggctatg caaacccaat taactgaaga gatgcggcag aacgcgcgcg
     5041 cgctggaagc cgacagcatc ctgcgcgcct gtgttcactg cggattttgt accgcaacct
     5101 gcccaaccta tcagcttctg ggcgatgaac tggacgggcc gcgcgggcgc atctatctga
     5161 ttaaacaggt gctggaaggc aacgaagtca cgcttaaaac acaggagcat ctcgatcgct
     5221 gcctcacttg ccgcaattgt gaaaccacct gtccttctgg tgtgcgctat cacaatttgc
     5281 tggatatcgg gcgtgatatt gtcgagcaga aagtgaaacg cccactgccg gagcgaatac
     5341 tgcgcgaagg attgcgccag gtagtgccgc gtccggcggt cttccgtgcg ctaacgcagg
     5401 tagggctggt gctgcgaccg tttttaccgg aacaggtcag agcaaaactg cctgctgaaa
     5461 cggtgaaagc taaaccgcgt ccgccgctgc gccataagcg tcgggtttta atgctggaag
     5521 gctgcgccca gcctacgctt tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc
     5581 gtctggggat cagcgtcatg ccagctaacg aagcaggctg ttgtggcgcg gtggactatc
     5641 atcttaatgc gcaggagaaa gggctggcgc gggcgcgcaa taatattgat gcctggtggc
     5701 ccgcgattga agcaggtgcc gaggcaattt tgcaaaccgc cagcggctgc ggtgcgtttg
     5761 tcaaagagta cgggcagatg ctgaaaagcg atgcgttata tgccgataaa gcgcgtcagg
     5821 tcagtgaact ggcggtcgat ttagtcgagc ttctgcgcga ggaaccgctg gaaaaactgg
     5881 caattcgcgg cgataaaaaa ctggccttcc actgcccgtg taccctacaa catgcgcaaa
     5941 agctgaacgg cgaagtggaa aaagtgttgc ttcgtcttgg atttacctta acggacgttc
     6001 ccgacagcca tctgtgctgc ggttcagcgg gaacatatgc gttaacgcat cccgatctgg
     6061 cacgccagct gcgggataac aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg
     6121 tcaccgccaa cattggttgc cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgtc
     6181 actggattga aattgtagaa caagcccttg aaaaggaata acaaaatgaa aactaaagtc
     6241 attcttagcc agcaaatggc gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa
     6301 aacaactggt ctgtttccat tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt
     6361 cgcatggacg attgcgcgcc gattgcggct tacatctccc aggagaaagc gcgtaccgcc
     6421 gcgctggggc gtcgtgaaac taagggctat gaagagatgg tgaacaacgg acgtaccgcg
     6481 ttcgtgactg cgccgttatt aacgtcgctg gaaggcggcg taccggttgt tgttgatggg
     6541 caaattattg gtgccgtggg cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg
     6601 aaagcggcag cagcggtgtt ggcgaaataa gcgaaaacga ggagagaaac aatgagtcaa
     6661 accataaccc agggccgttt acgcattgac gccaatttta aacgttttgt ggatgaagaa
     6721 gttttaccgg gaacagggct ggacgctgcg gcgttctggc gcaattttga tgagatcgtt
     6781 cacgatctgg caccagaaaa tcgtcagttg ctggcagaac gcgatcgcat tcaggcggcg
     6841 cttgatgagt ggcatcgcag caatccgggg ccggtaaaag ataaagcggc ctataaatct
     6901 ttcctgcgtg aactgggcta cctggtgccg caaccggagc gcgtgacggt ggaaaccacg
     6961 ggcattgaca gcgaaatcac cagtcaggcg gggccacagc tggtggttcc ggcaatgaac
     7021 gcccgctacg cgctgaacgc ggcgaacgct cgctggggct cactgtacga tgcgttatac
     7081 ggcagcgaca tcatcccgca ggaaggggcg atggccagcg gctacgatcc gcaacgcggt
     7141 gagcaggtta tcgcctgggt tcggcgtttc ctcgatgaat ctctaccgct ggaaaacggc
     7201 agctatcagg atgtggtggc gtttaaggtg gtcgataaac aattacgcat ccagttgaaa
     7261 aatggtaaag aaaccacgtt acgtaccccg gcgcagtttg tcggttaccg tggcgatgcc
     7321 gctgcgccga cctgcatttt gctgaaaaat aacggcctgc atattgaact gcaaattgat
     7381 gccaacgggc ggattggcaa agacgatccg gcgcacatca acgatgttat cgtcgaagcg
     7441 gccatcagta ccattctcga ctgcgaagat tcggtcgcgg cggttgatgc ggaagataaa
     7501 atcctgctgt accgcaacct gctgggcctg atgcagggga ctctgcaaga gaaaatggag
     7561 aaaaacggtc gacaaatcgt gcgtaaactg aatgacgatc gtcattacac cgccgccgat
     7621 ggctctgaaa tttctctgca cggacgctcg ctgctgttta tccgcaacgt gggtcatttg
     7681 atgaccattc ctgtgatttg ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat
     7741 ggcgtcatga ctggcgcgat tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact
     7801 ggcagcgtct atattgtgaa accgaaaatg cacggtccgc aggaagtggc gttcgccaac
     7861 aaactgttta cccgcattga gacaatgctc ggtatggcac cgaataccct gaaaatgggc
     7921 attatggatg aagaacgtcg gacctcgctg aacttgcgta gctgtatcgc tcaggcgcgc
     7981 aaccgcgtgg cgttcatcaa taccggtttc ctcgaccgta ccggcgatga aatgcattcg
     8041 gtgatggaag ctggcccgat gctgcgtaaa aaccagatga aatcgacgcc gtggatcaaa
     8101 gcctacgaac gtaataacgt gctttccggt ctgttctgtg ggctgcgcgg taaagcgcaa
     8161 attggtaaag gcatgtgggc aatgccggac ctgatggcag acatgtacag ccagaagggc
     8221 gaccaactgc gtgccggggc aaacacagcc tgggttccgt caccaaccgc tgctacgctc
     8281 catgcgctgc actaccacca aaccaacgta cagagcgtgc aagccaacat tgcccagacc
     8341 gagttcaatg ctgaatttga accgctgctg gacgatctgc tgactattcc ggttgctgaa
     8401 aacgctaact ggtcggcgca agagatccaa caagagctgg ataacaacgt gcaggggatt
     8461 ctggggtacg tggtgcgctg ggtggagcag gggattggtt gttcaaaagt gccggatatt
     8521 cacaatgtgg cgctgatgga agaccgcgca acgctgcgta tctccagcca gcatatcgcc
     8581 aactggttac gtcacggtat tctgaccaaa gaacaggtgc aggcgtcgct ggagaatatg
     8641 gcgaaagtgg ttgatcagca aaacgctggc gatccggctt atcgtccgat ggcggggaat
     8701 ttcgctaact cgtgtgcttt taaagctgcc agcgatttga tcttcctcgg cgtgaaacag
     8761 ccaaacggtt ataccgaacc gttattacac gcctggcgtt tacgcgaaaa agaaagtcat
     8821 taatagcgtc aaaaaagccc cggcaagttt tattgtcggg gctatgatag taactgcggt
     8881 agtacattta tcccggaaat aaccggagcg attatcaggg tttaacaata ttaaataccc
     8941 gtagcgcatt actagcgggc ctaaaacaac aatatggaca tcttaacgat actctgcgcc
     9001 tgcgtgtgcg gaacagaaac ccttacccga tatacttgtt atgccagagg tagcaattgc
     9061 gttagcggcg tactgttgct gtaaagtata acgcctatat tttacagttc agtcctcttt
     9121 tgcttattac aaccttaagt atatcaagca tataaagata ataagagact gaacaatatg
     9181 gttacctgga cccaaatgta tatgccgatg ggaggactgg ggctatccgc tctggtcgcc
     9241 ctgatcccga taatattctt cttcgttgca ctcgcggtat tacgtctgaa aggacatgtc
     9301 gctggagcaa taacccttat attatctatc ctgattgcaa tattcgcctt taaaatgccg
     9361 attgatatgg catttgctgc tgcgggctat ggctttattt atggattatg gccaattgca
     9421 tggattattg tcgcggcggt attcctctat aaattaaccg ttgccagcgg gcagttcgat
     9481 attatccgca gctcggttat ctccatcacc gacgatcagc gtttgcaggt gttactgatt
     9541 ggtttctcct ttggtgcgtt gctggaagga gcggctggct ttggtgcgcc ggtggcgatt
     9601 accggtgcgc tgctggtggg cctgggcttc aaaccgttat acgcggcggg gctgtgtctg
     9661 attgccaata ctgcgccggt ggcgtttggt gcgttgggcg tgccgattct ggtcgccggt
     9721 caggtaacgg gaatcgatcc gttccacatt ggcgcaatgg cgggacgtca gttaccgttc
     9781 ctgtcggttc ttgtgccgtt ctggctggta gcaatgatgg acggctggaa aggggtgaaa
     9841 gagacgtggc cagcggcgct ggttgctggg ggaagcttcg ctgtcactca gttctttacc
     9901 tctaattata ttggtccgga actgccggat attacttcgg cgctggtgag tatcgtctca
     9961 ctcgctttat tccttaaagt ctggcggccg aaaaataccg aaacggcaat cagcatggga
    10021 caatccgcag gtgcgatggt ggtaaataag ccatcttctg gcggtcccgt gccttcagaa
    10081 tatagtctgg ggcaaatcat tcgagcgtgg tcaccgtttt taatcttaac ggtgctggtc
    10141 accatctgga ccatgaagcc gtttaaagcg ttatttgctc cgggcggcgc gttttattca
    10201 ctggtgatta atttccagat ccctcatttg catcaacaag tgttgaaagc ggcacccatt
    10261 gtcgcccaac caacgccaat ggatgcggtg tttaaattcg accccctctc ggctggcggc
    10321 accgctattt ttattgcggc gattatctct atcttcatcc tcggtgtggg gatcaagaaa
    10381 ggtattggcg tctttgccga aacgctaatt agtctgaagt ggccgatact gtcgattggc
    10441 atggtgctgg cgttcgcctt cgtcaccaac tattctggca tgtccaccac gctggcgctg
    10501 gtactggcag gtacaggcgt gatgttcccg ttcttctcac cgtttctcgg ctggctgggc
    10561 gtattcctta ccggctcgga cacctcctct aacgccctgt ttggttcact gcaatcgacc
    10621 acggcgcagc aaatcaacgt ctctgacact ctgctggtgg cagcaaatac cagcggcggc
    10681 gtaactggca agatgatctc cccgcaatct atcgccgtgg cctgcgccgc gacgggcatg
    10741 gtgggccgag aatctgaact gttccgctac accgtgaagc acagtctgat ttttgccagc
    10801 gttatcggca ttatcaccct gctgcaggcg tatgtgttta ccgggatgtt agtctcgtaa
    10861 atcaataaag ccggatgatc gtcaatatca tccggcttca tttacattat tctgtcttat
    10921 taattgaaaa tcatttctcg ctttattatc catgcagcaa taatgttttg cgttcagata
    10981 acctgctaaa taattaagag cg
//
LOCUS       DAZGBC010000159        10924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGBC010000159 DAZGBC010000000
VERSION     DAZGBC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.160"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..179
                     /locus_tag="ACHK1J_004084"
     CDS             <1..179
                     /locus_tag="ACHK1J_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913287.1"
                     /translation="YEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGED
                     FRNHLQYKLTLPTH"
     gene            390..713
                     /gene="ybjQ"
                     /locus_tag="ACHK1J_004085"
     CDS             390..713
                     /gene="ybjQ"
                     /locus_tag="ACHK1J_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO7913288.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            710..1540
                     /gene="amiD"
                     /locus_tag="ACHK1J_004086"
     CDS             710..1540
                     /gene="amiD"
                     /locus_tag="ACHK1J_004086"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO7913289.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     STWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMRPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1537..2550)
                     /gene="ybjS"
                     /locus_tag="ACHK1J_004087"
     CDS             complement(1537..2550)
                     /gene="ybjS"
                     /locus_tag="ACHK1J_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7913290.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2649..4079)
                     /gene="ybjT"
                     /locus_tag="ACHK1J_004088"
     CDS             complement(2649..4079)
                     /gene="ybjT"
                     /locus_tag="ACHK1J_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO7913291.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGESGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLSLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4090..5091)
                     /gene="ltaE"
                     /locus_tag="ACHK1J_004089"
     CDS             complement(4090..5091)
                     /gene="ltaE"
                     /locus_tag="ACHK1J_004089"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO7913292.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLNKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5128..6846)
                     /gene="poxB"
                     /locus_tag="ACHK1J_004090"
     CDS             complement(5128..6846)
                     /gene="poxB"
                     /locus_tag="ACHK1J_004090"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO7913293.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(6979..7947)
                     /gene="hcr"
                     /locus_tag="ACHK1J_004091"
     CDS             complement(6979..7947)
                     /gene="hcr"
                     /locus_tag="ACHK1J_004091"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO7913294.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFITGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7959..9611)
                     /gene="hcp"
                     /locus_tag="ACHK1J_004092"
                     /gene_synonym="priS"
     CDS             complement(7959..9611)
                     /gene="hcp"
                     /locus_tag="ACHK1J_004092"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO7913295.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9755..10654)
                     /gene="lysO"
                     /locus_tag="ACHK1J_004093"
     CDS             complement(9755..10654)
                     /gene="lysO"
                     /locus_tag="ACHK1J_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO7913296.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2641 a         2902 c         2906 g         2475 t
ORIGIN      
        1 tatacgaatt tgcaagaatg atcttcgctt atattaatga caaaagcatt gagatcttat
       61 tactttcttg ttttgcggcg gcggaccaaa aagcaatcca gcggtatata cctcaggcgc
      121 aagatgggga agactttagg aatcatttac aatataagtt gactcttccc actcattaat
      181 gttgccggca caacataata gggcaggtaa gcggtgctcc ataatgaaac caatatccgg
      241 ttacgaaaca gataattgta caggcttatg tagctgtttt tgacacaatg agatgatttc
      301 ctaaactgat caaaaccttc tttccaacat ggcatatcag tccgataatg ctttagagat
      361 cgaaggctta acaaacaagg agatcgatca tgcaattttc cacaactcca actctggaag
      421 gccagaccat cgttgaatat tgcggtgtgg tgaccggcga agcgatttta ggtgccaata
      481 ttttccgtga tttctttgcc ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg
      541 aaaaagaact gcgtaaagca cgggagatcg cctttgagga attaggctcc caggcgcggg
      601 cgctgggggc cgatgccgta gttggtattg atatcgacta cgaaacggtc gggcaaaacg
      661 gcagtatgct gatggttagc gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt
      721 cttctggctg gtcgctgccg ctctgttact ggcagggtgt gcaggcgaaa aaggcattgt
      781 cgagaaagag ggataccagc ttgatacccg acgccaggcg caggcggcgt atccgcgcat
      841 taaagtgctg gtgatccact acaccgcaga tgattttgat agctcgctgg cgacactgac
      901 cgataagcag gtcagctcgc attatctggt ccctgcggta ccaccgcgat acaacggtaa
      961 accgcgcatc tggcaactgg tgccggaaca agaactggcc tggcatgcgg ggattagcac
     1021 ctggcgcggg gcaacgcgcc ttaacgacac ctctattggc attgagctgg aaaaccgtgg
     1081 ctggcaaaaa tcggccggag tgaaatattt tgccccgttt gaaccggcac agattcaggc
     1141 gcttattcca ctggcgaaag atattattgc ccgttatcac atcaagccgg aaaacgtagt
     1201 ggcacatgcg gatatcgcac cgcagcgcaa agacgatccg gggccattat ttccctggca
     1261 gcaactggcg cagcagggga ttggtgcctg gccggatgcg cagcgggtga acttttacct
     1321 tgccgggcgc gcgccgcaca caccggtaga taccgcgtca ttgctggagc ttttggcgcg
     1381 ctacggttat gacgttaaac ctgatatgag accgcgcgag cagcggcgcg tgattatggc
     1441 gttccagatg catttccgcc cgacgttata caacggcgaa gcggatgcag aaactcaggc
     1501 aattgccgaa gcgttgctgg agaaatacgg gcaggattag cgcggcagtt ttccgtggtc
     1561 gcgtagccag gcggcagttt tctcgatacc ttcatccagg gtgatgaccg gctgataacc
     1621 taattcttcc tgtgcacgcg taatatccag cgtaaagtca aaattaagct tcgagacgcc
     1681 gtagtgggtc agcggcggct cttttgctga cttgcggcct aaacgctcca tgctgcgggc
     1741 gatcatatcc agcatcgggt aggggacgga acgaatacga cagtcaatat tcaactcgtc
     1801 gatcagcttc tgcacgatgc tgcgcagtgt gcgatgctcg ccgttggtaa tgttgtacac
     1861 acgcccggaa ggtagcttat cgcaggcttc ctggcttgcc agccacatgg cgtgcactgc
     1921 attttcatag taggtcatat cgaccagcgc acttccgccg tgcggtaaca gaatgctgcc
     1981 gtagtggtgc atcatatgcg caagacgggg gataaagact ttatcgtgcg gtccgaacag
     2041 actttgtggg cgcagaatag taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat
     2101 cacttcttcg ctggctgctt tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa
     2161 atcttcttta atatcgcgat ggtggtgata atcgaagtac agggagggag aagagatatg
     2221 aataaagtta cgcacacccc aggcgacagc ccattcaccc aggcggcgag tggcgcgaac
     2281 gttagccaga tcgaaagcct gttgtgtccc ccagggagag gtaaagctgg agcagtgcca
     2341 cagcgtatca atgcccgcga gcatcacttt agcttgtgat gagaccagct cggtcagatc
     2401 cgccggaaca aactctgcgc ccattttttc cagcaattta cccattgcct cgttgcgacc
     2461 ggtcgctcgc acgctgatgc ctttctggca taaaaactct accgcgtttc gacctaagcc
     2521 gctggtggcg ccggtaacca gtaccttcat atcaatccac tgttgttgag aaaatgacgt
     2581 gcgcattctt ccgtgatttc ccccatgatg caatgggaaa catgaaagaa taacgcaggt
     2641 tttgtcgatt aatctgtgct ttgttctgcc agtctggcga tttgttttgc cattccgcga
     2701 aaaataaaca gatgcgcggg gatcatcaat aaccagtaaa acagccccgg cataccgtgc
     2761 ggatgccaga aagcgcggac atcgatagta cgatagtcgc ctttatcttc cagggtaaaa
     2821 cacagtcgtc ccagccccgg tgctttcatg ccaaataaca acgaaagttg ctttttcggt
     2881 tcaacgacaa tgactttcca gctatccacc gcatcgccag tttgcaaata ttcgcgctcc
     2941 ggacggcctt tcgctaattt atgaccgatc gcacggtcca tcaacgcccg tgtctgccac
     3001 aaaatattgc caaagaaata acgctcttta ccgccgattt ggttcactac ctgccataaa
     3061 gccgcaaggc tggcggacgt tttaacggta aaccccgcct gtttggcgaa ataaccgtac
     3121 tcaggtcgcc agcgggcaaa ggcctgagcg tcgtagcccc agtcgctgga gttgaccagt
     3181 ttttcttcct ctttcaacgt gctacgtacc gcgtcatcga aagcgatcag ccgttgtggg
     3241 atgagtgcac gtagcgcggt gtcatccgcc agcagatcgt gtttcagccc ctgaatcaac
     3301 gccctggcgg tggtgggcgg tacggaagta atcacattaa gaaaccacac cgaaatccag
     3361 cgagtgggga gggggatggg gatcaaccag cggcgcttac cgctcaccgc cataaaatgt
     3421 tcaaactgtt gctgataact gagcacctct ggtccggcgg cttcgaagat gcggtgttcg
     3481 ctggccggat gatctaacag cgccaccaga tagtgcagca agttttccag cgcgatgggc
     3541 gtggtgcgtg aacgaaccca gcgtggcggc gttaacactg gcagattgta gaccatatcg
     3601 cgcatgactt cgaacgctgc tgaacctgcg ccaacgataa ttccggcccg cagttcggtc
     3661 accgggacac ctgcttcacg aagaatatcc gccgtagcct gacgagcacg cagatgatcc
     3721 gactgctcat gtggcggggc ctgtaacgaa ctgagaaaga ttaattgctt aactggtact
     3781 tcacgtagcg catcgcggac gttgagagcc acctggcgct cctgagcgat aaaatcgccg
     3841 ctttcgccca tgctgtgcac cagaaaatag acggtatcga tatcctgtaa cagggccgga
     3901 aggttatccg gccagctgag atcgacttta tggcaactga cgtttgccag ttgcagcttt
     3961 gcaagcctgt cgacatgacg tgccgccgcc aggatctgat gcccttgctg gctgagtgtg
     4021 cgcaccagat gctgaccaat gtagccactg gcaccgagaa ctaaaatgcg ttgcggcacg
     4081 tttctctcct taacgcgcca ggaatgcgcg ccagtgggcg gcgacttccg ccagctgttc
     4141 gcgcgagacg tcaagatgcg tcaccaggcg gacaatcggc gaggcgttaa tcagcacgtt
     4201 tctcgctttc atgtattcgc ctaacgcggc agcattttct tccccgacgc gaacaaacag
     4261 catattagtg tcctgacgca tcacatccgc gcctgcttca cgcagctgct ccgccatcca
     4321 ggcagcgttg tcgtggtctt cctgcaagcg cgcaacatta tttttcaggg catatatccc
     4381 ggcggcagcc agaatgccgg actggcgcat cccgccacct gtcattttcc gccagcgaat
     4441 ggcacgttta atgtaatcac gattaccgac gagtaatgaa ccgactggcg tcccaagacc
     4501 tttcgacagg caaatggtga acgaatcaca atattgcgtg atctctttca gttcgcagcc
     4561 gtaagccacc acggcattaa agatgcgcgc accgtcaaca tgcagcgcca gattgcgctc
     4621 gcgggtaaat tcccatgcat ctttaaggta ttcacgcggc aacactttgc cgttgtgggt
     4681 gttttccaga ctgagtaatt tggtgcgggc gaaatggata tcgtcgggtt tgattttcat
     4741 cgccacttta ttcagcggta gcgtgccgtc ggcagccgcg tcgatgggtt gcggctgaat
     4801 actgcccagc accgctgcgc caccggcttc aaacagatag ttatgcgcgg cctgaccgac
     4861 aatatactct tcgccgcgtt cgcagtgact gagcagagcg accaggttgg cctgagtgcc
     4921 ggtaggcaga aaaatggcgg cttctttacc ggaaagctct gctgcatagt cctgcagagc
     4981 attaacggta gggtcgtctc cgtaaacgtc gtccccaacc ggggcggcca tcatcgcttc
     5041 gagcatggcg cggctcggtc gggttacggt atcactgcgt aaatcaatca tggcatgtcc
     5101 ttattatgac gggaaatgcc acccttttta ccttagccag ttcgttttcg ccagttcgat
     5161 cacttcatcg ccgcgtccgc tgatgattgc gcgcagcata tacaggctga aacctttggc
     5221 ctgttcgagt ttgatctgcg gtggaatggc taactcttct ttggcgacca ccacatccac
     5281 caataccgga ccgtcgatgg agaaggcgcg ttgcagggct tcatcaactt cagacgcttt
     5341 ttctacacgg atacccgtaa tgccgcatgc ttcggcaatg cgggcaaagt tggtgtcgtg
     5401 cagctcagta ccgtctgtca ggtagcctcc ggctttcatc tccatcgcca caaagcccag
     5461 cacgctgtta ttaaagacga taattttcac cggcagtttc atctgcacta ccgagaggaa
     5521 atcgcccatc aacatgctaa aaccgccatc gccgcacatg gcgaccacct gacgttccgg
     5581 ctcggttgcc tgcgcgccca gcgcctgcgg catggcgtta gccatcgaac cgtggttaaa
     5641 cgaacctaac aggcgacgct tgccgttcat tttcagataa cgcgctgccc acaccgttgg
     5701 cgtaccaacg tcacaggtga aaatggcgtc atcggcggca aaatgactaa tttgctgcgc
     5761 cagatattgc gggtgaatgg ctttctcgct cggtttagct aaatcgtcca gccctttgcg
     5821 ggcatcgcgg taatcttcca gcgctttatc cagaaatttg cgatcggctt tttcttccac
     5881 cagtggcagc aatgcacgca gggttgattt gatatcgccg accagtgcca tatccacctt
     5941 gctgtgagcg ccgatgctgg ctggattgat atcaatctga ataattttgg catcggtcgg
     6001 gtagaacgca cggtagggga actgagtacc gagtagcact aacgtgtcgg cgttcatcat
     6061 ggtatggaaa cctgacgaga atccgattaa cccggtcatt ccaacatcat acggattatc
     6121 gtattcgaca tgctctttac cgcgtagggc atgaacaata ggtgctttaa ttttcccggc
     6181 aaactcaact aactctttat gcgcccccgc gcagccgctg ccacacatca gagcgatatt
     6241 gctggaataa cgcagcagtt gcgccagttt gcgtaactct tcttcttccg gcgtcacaac
     6301 tggctgtggc gcatgatacc agtgcgtggt tgccccttct ggcgcaggtt ttaacgccac
     6361 gtcacccggt aacacgacaa cggaaacgcc acggttaagc accgctttgc gcatagcaat
     6421 tgccagcact tgtgggatct gctccgggct ggaaaccagc tcgcaatagt gactacattc
     6481 gcggaatagc tcttgtgggt gggtttcctg gaaatagccg ctgcctattt cgctggaggg
     6541 aatatgagcg gcaatcgcca gtaccggaac gtgattgcgg tggcaatcga acaggccgtt
     6601 gattaagtgc aggttgccgg ggccgcacga tccggcacag accgccagtt ctcctgaaag
     6661 ttgtgcttca gcgccagcgg caaacgccgc cacttcttca tggcgggtgg acatccactc
     6721 gatggtgccc atgcgattaa gactgtcgct aagaccgttc agagagtcgc ccgtgactcc
     6781 ccagatgcgt ttcacgcctg ccgattcgag tgttttggcg atataagctg caaccgtttg
     6841 tttcatggtt ctccatctcc tgaatgtgat aacggtaaca agtttagttc atctgacgga
     6901 gggggaaggg atgggagaaa aaggaggcac taacggttaa atagcccgat gaaatgaata
     6961 tcatcgggca taaggcgatt atgcgagaac caaatccccc tgcggatggc aggagcaggc
     7021 cagtacgtaa ccttcagcga tttcggcgtc ggtcagcgtc attgtgctgc tcaccgtata
     7081 ttcaccggaa accacttttg tcttacagca gccgcaaaca cccgcacggc aggcagcgac
     7141 aaccggaacg ttattgcttt ccagcgcctc cagtagcgta gtgccaaccg gggcgtaaaa
     7201 ttctcgtgcc ggttgcagtt tggtgaattt cagaccgctg gtcgccgctt ccgctactgg
     7261 ggtgaagaat ttctctttaa agaaacgcgt cacgccgagc gctttcactt cctgctctac
     7321 ccaatccata tacggtgctg gaccacaagt catcacggta cgtgaagcta agtcaggaac
     7381 gcgagtcagg agttcgcgag tgagacgacc agtgataaag ccttcggtaa cgttattttc
     7441 cgccaccagc gttaccggat agttacgcca ctcatcggcg aaaataacat cctgcggcgt
     7501 acgcacgttg tagatcacct gtacatcggc ctgtggacga ttcttcgcca gccagcgacg
     7561 catcgacata atcggcgtga cgccgcagcc tgcagccagc aacaggaatt tatcttctgc
     7621 tttatcgtcg caggtaaatt cccccatcgc gtccgaaagc cagagataat caccgcgttt
     7681 tacatcgcgg gtcagccact gggagccgac accgtcatca atccgccgca cggtcagggt
     7741 gatatattca ctcacgcctg gcgtggagga aatggtgtaa gcacgcagcg tttccgctga
     7801 gttacgcacg ctgaccagtg catattgccc ggcgcggtat gggtagtaat cgtggcaaat
     7861 cagggaaatc gtccacacat ccggcgtttc ttgcttaatg tgatgaacct gcatccgcca
     7921 cgggcattga ttcgttggca tcgtcatcga caaactcctt acgcgctcaa cagttgcttc
     7981 atgtcttctt caacagtggt gatagaacgc aggccgaatt tctcgttcag caccgccagc
     8041 agatctggtg tcaggaaacc aggtgcagtc gggccggtga cgatattttt cacgcccaga
     8101 gaaagcagcg tcagcagaat gacgatcgct ttctgttcaa accaggagag caccagcgac
     8161 agcggcagat cgttgacacc gcagcccagt ttctctgcca gcgtaaccgc cagaataatc
     8221 gctgagtaag catcgttaca ctgaccggca tctaccagac gcggcagacc ttcgatatcg
     8281 ccaaactcaa gtttgttaaa gcgatattta ccacaggcga gggtcaggat caggcagtca
     8341 tccggcacgc tggtggcgaa atcggtgaag tagtggcgct cgccgcgtgc gccgtcacag
     8401 ccaccaagca ggaagatatg acgcagtttt tcacggctca ccagatcaat cagcgtatcc
     8461 gctgcgccaa gcagcgtctg gcggccaaag cccacggtga tcaggtgcgg aatttcgctg
     8521 tacgggaagc ccgccatttg ttgcgcctgg gcaatgaccg cagagaaatc ttcgccatcc
     8581 agatgacgca cacccggcca gccaacgatg ctgcgggtcc agatacgatc gtcataagcg
     8641 cctacggttg ggtcgatgat gcagttcgag gtcatcacga tggggccagg gaaacgagcg
     8701 aactccactt gctgattctg ccagccgctg ccgtagttac cgaccagatg cttgaattta
     8761 cgcagctccg ggtagccatg cgcaggcagc atttcgccgt gggtgtagac attaacaccc
     8821 gtgccttcgg tctgttccag aaggttgtag agatctttga gatcgtgacc ggaaatcaga
     8881 atgcatttac ccgccgtcgc tttgacgttg acctgggttg gcgtcgggtg accgtattta
     8941 ccggtttcgc ctgcatccag aatgctcatc actttgaagt tcatctggcc gatttccatt
     9001 gaacactcaa gaagcgcgtt catatcggca ggccaggtcc ccagccacgc catgatttta
     9061 tggtactggg cataaatatc attgtcgtat tgaccgagaa cgtgtgcgtg ttccatatag
     9121 gccgccgcac ctttcaggcc atacaggcac agcagacgca ggccgagaat gttttcgcca
     9181 atcgccgctt tatctttgtt aggggtaaat tctgctgcct gacgttgcag ttcgccgaga
     9241 tcgtcgctca ccagttgcag gtcagccatc gggttatcga cgcgggcgtt ggcatctaca
     9301 gccaggcatt gtgctttcag cgcctcgcgc aaggcaatcg cttcacgagc gtagccgaca
     9361 atacgcggag aatcgaagtt aacgttggtc agggttgaga aaaatgcgcg tggcgcaaag
     9421 ctgtctacat cgtggttgat gatgccgtat tcacgcgctt ttaccgccca ggccgaaagc
     9481 ccttgcagcg ccgcgatgag taaatcctga aggtcagagg tttccgccgt tttaccacac
     9541 atcccctgcg cgtatgagca gccgtttcct gccggagtac ggatagtttg ttcacattgc
     9601 acacaaaaca tgatcacacc ttttaaagtt atatttaata tacatcttta aggttaagac
     9661 gcttaacgcg gggataaaag ggatttttca tgcaacttta agggagattg atttagcgca
     9721 attttggcgg cagggatcta ccgccagaga ggtattacgc agagaaaaag gcgatgagga
     9781 tcggcactaa caggctaaga ataaaaccgt gaacaattgc cgccgggacc atatccagcc
     9841 cgccagtacg ttgaagaacg ggcagggtga aatccatcga cgtcgcaccg cataagccca
     9901 gtgcggtaga gcggctgcgg cgaatcagcc cagggatcaa cataattgca atcagctcac
     9961 gggccagatc attaaaaaac gccgcgctcc cgattaccgg accaaaagat tcggtcaata
    10021 aaataccgga aagagaatac cagccgaaac cagaggccat tgccagcgcg gtattgatgg
    10081 gtagatcaag aataaaggcg ttaattaaac caccaattaa tgaactggca accaccacca
    10141 cggcgacaat cattccccgg cgattaagga caatctgctt taaggtcatg ccattattgc
    10201 gcaactgaat accgacgagg aaaagtagca gaattaaggt gtattcactg gcttcggtcg
    10261 cgtgttgtaa gaaagccagt ccactcaggc caatggcaaa accaatcact actacgccgc
    10321 acagttttag cgactcaagc gccatcgcaa tacgcgacgg gagtttttct tgctgatggt
    10381 ggttgcgcca cggcagaccg cgctccagcc acataagggc ggcaatatta cacagtaaaa
    10441 taacggtaat actgacggca gaataatgca gaatcgccaa caggttactg gcgaggttat
    10501 cgagaaacgc cagactgata cccataaaaa agagaataag gtaaaccatc cagcttagta
    10561 gctgattaat aacttttaac gcagcttgtt ggcgaagcgg aatgaggtaa cccacaatca
    10621 ggggaaccag aatgattaac aacccagaaa acatgaaaac ccagcccttg caaagaaaga
    10681 agtcgaaatg cgcgatgaca cactactgaa agcggaagga cgagtaaagt tgcaattaaa
    10741 aggaaatgtt atgcataagg atcggtaggg tattcatttt catttaaaga tattcttgct
    10801 ctttaattac aaaccgcacc ggtgttggtg gcgtcaaaat cgccgaggcg ttccctgaag
    10861 gccggggcag cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg
    10921 cccg
//
LOCUS       DAZGBC010000160        10730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGBC010000160 DAZGBC010000000
VERSION     DAZGBC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.161"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1181)
                     /gene="rhsD"
                     /locus_tag="ACHK1J_004094"
     CDS             complement(<1..1181)
                     /gene="rhsD"
                     /locus_tag="ACHK1J_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO7913297.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQVQRAEEARTSSLSSSDRSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYCYQYEQDRIT
                     VT"
     gene            complement(1611..4025)
                     /gene="ybbP"
                     /locus_tag="ACHK1J_004095"
     CDS             complement(1611..4025)
                     /gene="ybbP"
                     /locus_tag="ACHK1J_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO7913298.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMINLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LSEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMHQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(4022..4708)
                     /gene="ybbA"
                     /locus_tag="ACHK1J_004096"
     CDS             complement(4022..4708)
                     /gene="ybbA"
                     /locus_tag="ACHK1J_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO7913299.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            4679..5302
                     /gene="tesA"
                     /locus_tag="ACHK1J_004097"
     CDS             4679..5302
                     /gene="tesA"
                     /locus_tag="ACHK1J_004097"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO7913300.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5292..6101
                     /gene="ybbO"
                     /locus_tag="ACHK1J_004098"
     CDS             5292..6101
                     /gene="ybbO"
                     /locus_tag="ACHK1J_004098"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7913301.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            6162..7016
                     /gene="cnoX"
                     /locus_tag="ACHK1J_004099"
     CDS             6162..7016
                     /gene="cnoX"
                     /locus_tag="ACHK1J_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO7913302.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(7079..7858)
                     /gene="fetB"
                     /locus_tag="ACHK1J_004100"
     CDS             complement(7079..7858)
                     /gene="fetB"
                     /locus_tag="ACHK1J_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO7913303.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(7845..8522)
                     /gene="fetA"
                     /locus_tag="ACHK1J_004101"
     CDS             complement(7845..8522)
                     /gene="fetA"
                     /locus_tag="ACHK1J_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO7913304.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            8668..9585
                     /gene="qmcA"
                     /locus_tag="ACHK1J_004102"
     CDS             8668..9585
                     /gene="qmcA"
                     /locus_tag="ACHK1J_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO7913305.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            9582..10040
                     /gene="ybbJ"
                     /locus_tag="ACHK1J_004103"
     CDS             9582..10040
                     /gene="ybbJ"
                     /locus_tag="ACHK1J_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO7913306.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(10321..10683)
                     /locus_tag="ACHK1J_004104"
     CDS             complement(10321..10683)
                     /locus_tag="ACHK1J_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913307.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKGMGKTTEE"
BASE COUNT         2521 a         3014 c         2789 g         2406 t
ORIGIN      
        1 gtgacggtga tgcggtcctg ctcatactgg tagcagtaac tcaggcctgc cgggtttagt
       61 tgctccacca cccgccccgc atcgtcgtag cggtagcgca tctccggcct tcccgcgtaa
      121 cggtgtgcca ccatccgccc ggggtgctgc gcgtcatatg tgaaagcgcg cacctgcgta
      181 ttgctgcggt catataccgc cagcagttca ccggcctccg tatacgtgta ccgcaccagg
      241 ggcgcaccgg gcaggctctc cgggtacgcc gggtcgtgca tcagccacac cgccgaaagg
      301 cggatacccc tgtcggggcc gtattcggta ccgggcagtg tgtcggggaa cgctgaggct
      361 gagagagggc gggaacggtc agaagaagat agcgaagagg tgcgggcctc ttccgcacgc
      421 tgcacctgcg tggtcagcac cagacggaac tcccgcccgg caccgtccgt cacgccggtg
      481 atttccccgg ccaggtcacc ggcggcctca cgccggtacg tcagcgtccg cccgaagcgg
      541 tccgccatcc cggtaagcac ccggtacggc ggcagcggcg ctggcagtac gtcctcagca
      601 cccggcaccc gctcagacca ccccagtatc caccacggcc cctgtgcgct gttggtcgcc
      661 aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg acgcccacag ccgtgccagc
      721 gtgtggccgt ccggctgtgc tgccttacca ccgcgcacca gccacattga ctcgctgcgg
      781 ctgtacaccg cctcacccgg cagcagcggc tcaaagtgaa tgctccgtcc gccgttgtcg
      841 ttgagtacca gtgcatcatc gcgtagctgt aagcggatat cagaaggcgc tttccagccg
      901 gggccaaaaa tccccaccgg tgcaggcgtc ttcgtccggt agctgctgta ggtgcgggag
      961 agaatgaacg gcagcgggcc gggcagcgca aggtccgtct cgccgggcag caccttcgcc
     1021 cccagcagcg gatttaccgg gttgcccgag gtcatcccgc ccggacacac cgagcacgcc
     1081 acgccagttg gcgcgccaat tcttacacct gccgaaccct ggacaatggg accgccatac
     1141 tgagtcatat ctccctgacg cgccgctggt tttccgctca tataaagtgc ccttttagtt
     1201 attgttcata tccctgtgtt agcaacaaaa aaaataaaat gacacacaag accagctaat
     1261 gtatttatat atttttcaca taaacttatt tgttctcaat caacaacaac tgttattaca
     1321 ggtcactttc aatattttat taaactaaaa tttttagcac agatgaattc atcatcaaca
     1381 aattcactat atggataata tccatttaac gcatatgatt tatgatttta ttagtctgag
     1441 ttatttttgt agggctatat ttaaaattat taaactccac cgattcagat taatatcagc
     1501 aatagaaaga taggaaatca ccaattcaat aacatcatca atgaaacata ttgtagaatt
     1561 aatttaaaca aggcgttcca ttcaaccaga acgcccctta tttactctca tcaccccgca
     1621 aactgcctga acagcgcctt acccttaacc agtctcgcgc ccagccagcc gccgaaaagc
     1681 gacagcagca gcgcaccgct gcacggcaga acaatccaca atcgccagtc tggctcccac
     1741 gggaaatcaa acactttcgc ctgcaacact gccagcgccg tttccgcacc aattgcggcc
     1801 accaggccag aaacaaaccc aagcatggcg aactcgcacc acaatgtcgt gcgcagcagt
     1861 tttttccccg caccgagtgt gcgccacacc accagctcct gatgacgctg atgcattccc
     1921 acctgcacct gtgccagcaa cagcaacata ccgcaggcgg tgaccagcac caccatcacc
     1981 tccagcgccc gacttacctg ctccagcacc tggccgacct gttttaaaat cgcgccaatg
     2041 tctaacaggc taatggtcgg gaactggcgg ttgagttgcg tcaacatgcc gttgccattc
     2101 tcccagcgga aactggtaag ccagctctgc ggttgcccgt ccagagcccc ttcagggaaa
     2161 ataaaataga aattaggccg cagactttcc cagtcaactt tgcgcaggct ggtcacttta
     2221 gcgcggaact cctgggtatc gcccataaaa gtcacggtat caccgagggc aacgtttaag
     2281 cgtttcgcca gcccctcttc catcgacact tcatcggctt ttggcggcca gttaccggcg
     2341 acaatcggat tatgatcggg ccgcgtattt tgccaggtaa gattgagttc gcgattgagc
     2401 gcctcatctt cattgccttc tgtcggcttg ccgttaatcg ccgtcagtct cgcccgcacc
     2461 accggataaa acgattccgg gactatctga tgttccgaga ggaacgcttt tagcggtgct
     2521 acctgttctg tggcgatgtt aattaaaaag tagttcgggc tttctggagg tagctgctgc
     2581 tgccagcggt cgagcagatc gccacgcaac accagcagca gtgccagcag cataaaggag
     2641 agcgaaaatg ccgaaagctg gcttaacgtt gaccacggct gacgtaacag gcggctaacc
     2701 gccaggcgca gaggcagcga tttcagcgtc atacggcgaa gtacattcag cagcatccag
     2761 cccagcacac cgcacagcaa agccagtact accgcgcccg ccagcaccgc ccaaagcagc
     2821 atgctgccac ccattaatcc ggcgagcagc agcacaacca ccacactgac aatcggcaga
     2881 taaaacttca gcggccagac gttcgcaacc acatcattac gtaatacgcg taaaggctgc
     2941 gttgccagca acaagcggta tggtcgtagc cccaccagca gcgagatgac cgtcatggtg
     3001 ccgagcgccc acagccatgg ccagaggctg gctggcggta gtgcagcagg cagaacgggc
     3061 ttgagcagca ccatcaacac gttttcgaac aacaggccta tcgccccacc ggtaacggct
     3121 gaaagcgtca gcaccatcaa ccactgaccg acgattagct tacgcagttg cgctcgccct
     3181 gcccccagcg ttttgaggat cgccaccaga tcgtagcgac tgcgacagta atgattcatc
     3241 gccaccgcca ccgctgccac tgccagcagc aaggtcagaa gcgccgaaag cagcaggaac
     3301 tgctgcgagc gttccatcga tcgccccagc gcgccttcgt cctgttccag accgtaccag
     3361 cgttgttcgg gtttaagctg aggtaacaac catttctcat agctgtcgag ctggttctcg
     3421 ctgccgccga atttatagcg ccaggtgacc cgactccccg gctgcacggc tccggtttta
     3481 tcgacatccg ccagattaat catcagacgc ggagccatct ggaaggggtt aaaaccggaa
     3541 tccggttcct gaatcacttc tccggcaatc cgcaaggtgg catcgcccac gtcaatggtg
     3601 tcgcccgttt tcaggttaag cagtgccatc aggcgtgggg ccagcaatac gctgcccgcc
     3661 tgcggtttca ggccaggggg attggtttgc agatcgccat acatcgggta gatatcatcc
     3721 accgctttga cgttcgccag ctgcggtgtg tcgcctgcaa atgtcatggt ggcgaaagtc
     3781 agctgcttgc cgactttcag gccgcgcttt tgcgcttcct ccagccacgc ttgcggcact
     3841 tcgcgcgaac tgcgcagcgc ccgatcgccc gccataaact cacggctttg ctggcttaag
     3901 cccttctcca tgcgatcgct gatattgccc agcgccagca cgcaggccac cgccaggctt
     3961 agcgccagcc agacaattaa tagcgacggc gagcgccatt cgcgccagaa ccaacgtgca
     4021 atcatgcttc ctcctgcaac tgcccgttca ccagccgtaa gcagcggtcg cagcgtgccg
     4081 ccagttgcag gtcgtgggtc accattatca acgtggtgcc atgctcacgg ttgagggaaa
     4141 acagcaagtc ggcaatttta tcgcccgtct ggcggtcaag gttaccggtg ggttcgtcgg
     4201 caaacagcac atcaggtcga ccgttaaagg ctcgtgccag cgccactcgt tgctgttcac
     4261 cgccggaaag ctgtgccgga agatgatcca gacgtttacc cagccctaac tgttcgagca
     4321 acgctttcgc tccgttacga ctttccgcgc tactctcacc gcgcagcagc gccggaagct
     4381 cgacgttttc cagcgcgtta agggtaggaa ttaacataaa tgactgaaaa acaaagccga
     4441 cgtgcttcgc gcgcaacttt gcccgcgctt cttcgtccat attatgtagc ggttgtccca
     4501 ccagactcac ttcgccactg ctgccgtcat ccagcccggc gaggatcgcc agcaaggttg
     4561 acttacccga tcccgactcg cccaccagtg cgatggtctc gccacgtttg acaaccagct
     4621 caactccggt gaggatggag agttcatgct ccccctgacc gacggacttc ttaagatgat
     4681 gaacttcaac aatgttttcc gctggcattt gcccttcctg ttcctggtcc tgttaacctt
     4741 ccgtgccgcc gcagcggaca cgttattgat tctgggtgat agcctgagcg ccgggtatcg
     4801 aatgtctgcc agcgcggcct ggcctgcctt gttgaatgat aagtggcaga gtaaaacgtc
     4861 ggtagtcaat gccagcatca gcggcgacac ctcgcaacaa gggctggcgc gccttccggc
     4921 tctgctgaaa cagcatcagc cgcgctgggt gctggttgaa ctgggcggca atgacggttt
     4981 gcgtggtttt cagccacagc aaaccgagca aacgctgcgc cagattttgc aggatgtcaa
     5041 agccgccaac gctgaaccat tgttaatgca aatacgtctg cctgcaaact atggtcgccg
     5101 ttataatgaa gcctttagcg ccatttaccc caaactcgcc aaagagtttg atgttccgct
     5161 gctgcccttt tttatggaag aggtctacct caagccacaa tggatgcagg atgacggtat
     5221 tcatcccaac cgcgacgccc agccgtttat tgccgactgg atggcgaagc agttgcagcc
     5281 tttagtaaat catgactcat aaagcaacgg agatcctgac aggtaaagtt atgcaaaaat
     5341 cggtcttaat taccggatgt tccagtggaa ttggcctgga aagcgcgctc gaattaaaac
     5401 gccagggttt tcatgtgctg gcaggttgcc ggaaaccgga tgatgttgag cgcatgaaca
     5461 gcatgggatt taccggcgtg ttgatcgatc tggattcacc agaaagtgtt gatcgcgcag
     5521 ccgacgaggt gatcgccctg accgataatt gtctgtatgg gatctttaac aatgccggat
     5581 tcggcatgta tggccccctt tccaccatca gccgtgcgca gatggaacag cagttttccg
     5641 ccaacttttt cggcgcacac cagctcacca tgcgcctgtt acccgcgatg ttaccgcacg
     5701 gtgaagggcg tattgtgatg acatcatcgg tgatgggatt aatctccacg ccgggtcgtg
     5761 gcgcttacgc ggccagtaaa tatgcgctgg aggcgtggtc agacgcgctg cgcatggagc
     5821 tacgccacag cggaattaaa gtcagcctga tcgaacccgg tcccattcgt actcgcttca
     5881 ccgacaacgt caaccagacg caaagtgata aaccagtcga aaatcccggc atcgccgccc
     5941 gctttacgtt gggaccggaa gcggtggtgg acaaagtacg ccatgctttt attagcgaga
     6001 agccgaagat gcgctatccg gtaacgctgg tgacctgggc agtaatggtg cttaagcgcc
     6061 tgctgccggg gcgcgtgatg gacaaaatat tgcaggggtg agttgaagcg cgcgcttaag
     6121 cccccatgtc aaagaaaata tcgacaacag agagcgactc catgtccgta gaaaatattg
     6181 tcaacattaa cgaatctaac ctgcaacagg ttcttgaaca gtcgatgacc actccggtgc
     6241 tgttctattt ttggtctgaa cgtagccagc actgtttgca gttaacccca attctggaaa
     6301 gcctcgcggc gcagtacaac gggcaattta ttctggcgaa gctggactgc gacgcggagc
     6361 agatgattgc cgcccagttt ggtctgcgcg cgattccgac cgtgtatctg ttccagaacg
     6421 ggcaaccggt agatggcttc caggggccgc aaccggaaga ggcgatccgc gccctgctgg
     6481 ataaagtgct gccgcgcgaa gaagagctga aagcgcagca ggcgatgcaa ctgatgcagg
     6541 aaggcaatta caccgacgcc ctgccattgc tgaaagacgc ctggcagttg tcgaatcaga
     6601 acggggagat cggcctgctg ctggcagaaa cgctgattgc gctgaaccgt tctgaagatg
     6661 cggaagcggt gctgaaaacc attccgttgc aggatcagga cacccgctac caggggctgg
     6721 tggcgcaaat cgaactgctg aagcaggcgg ctgatacgcc ggaaattcaa cagttgcaac
     6781 agcaggtggc ggagaatcca gaagatgccg cactggcgac gcaactggcg ctgcaactgc
     6841 atcaggttgg gcgcaatgaa gaggcgctgg agttgctgtt cgggcatctg cgtaaagatc
     6901 tcaccgccgc agacggtcag acgcgtaaaa cgttccagga gatcctcgct gcgctgggta
     6961 cgggtgatgc actggcgtcg aagtatcgcc gccagctgta tgcattgttg tattgatatt
     7021 ccgttgatcg gcacatgaac cacaacgccg gatgcgatgc tgcgcatccg gcatcgcatc
     7081 atttcttctt caattgcgtc accaccaact ggtggcgcga attataaaac ttacgatagg
     7141 ttaaatagca ggcaataatg gtcgacaagc tggcggttga gagcagcata aaggtcacca
     7201 taatctgata tttaatcgcc ttcaccggat caatcccggc aaatatcagc ccggacatca
     7261 ttcctggtaa actcactaag ccaaccgttt ttgctgaatc gaccgtcgga attaaagccg
     7321 cgcgaatact gtcgcgaatc aatatcgctg aagcctgttt cggcgtcgcg ccaagactca
     7381 gtttctcctg aatctgttgc tgttcgctaa tcacccgttg tcctaaattg ttgtaacaca
     7441 gccctaccgc taccatggcg ttaccggcta tcatcccggc gatagggatc acctgcatcg
     7501 ggataaattc aatcgaccct gagagaatca gcaccgccag ggtaattccc gccccgaccg
     7561 taatggcaat aaacgatgag ataaaagctt tagcaatata tttactgcgt ttttgcgcgt
     7621 tccacgccgc attaaagcag ataaataaca ccatcaataa tgtcaggctg gcatcatcca
     7681 cgctgaaaat atacttcagc acatagccga caataatcag ctgaattatc gcgcgcccga
     7741 cgctccagag aatatctttc tccagcgcca gtttttcttt atggctaatt aagattgcca
     7801 ccaccaccag cattaatgcc agtgctaatg attcgttagt aatattatgc gagttcatag
     7861 cgtgcttcct gcatttctcc ggcatgcggt tgcagtgtaa tcactttatc cgcatgatta
     7921 atttcgtctt tatcgtgtgt cacccacagc acggcaatat tttgctcgcg cacataacga
     7981 tggatcatct cattgacgtt atgtttatta ctttcatcca gcgcactggt tatttcatcc
     8041 agcaataaaa ccttcggcat aaattgcagg ttacgaatca atgagatgcg ttgtttttca
     8101 ccaccagata gctcggcgat attcttcgtc aaaatgctgt ccggcaaggc gaagcgttca
     8161 agaaaatcga gaaaaatggc cgggtcaggc tgccggttac ggatctgcca gggaaagatc
     8221 agattatcgt ataccgtatc gccaaacagc gtcggtgtct gggcgcagta agagacttgt
     8281 tggcggtaga tttctggctt tagtgtgctg acatcctcac cttcaaacag taacgttccg
     8341 ctggttgggc tgatcaatga agcaactatt tttagcagcg tacttttgcc acaaccagaa
     8401 ggaccggtaa ttaacttaaa ttcgccagca cgcagcgaaa aattgatgtt attaagaatc
     8461 ttcgcatcac ccgccagata tcctacgttt tgtagctgaa gcaaaggact attttcctgc
     8521 atcgctgttc cctttttctg atttttacta aaaacagttt atccttcgca ggaataaggg
     8581 ggaactctct ttcagtaatc aggtataatt tgtttaatca agcagcaatt ttgctgatta
     8641 tggacggtac aacaggaggt ttttccgatg cttatcttta tcccgattct catttttgtc
     8701 gcgctggtca ttgtcggcgc gggcgtcaaa atcgtgccgc agggctatca atggacggta
     8761 gaacgttttg gtcgctatac caaaacgtta cagccggggc tcagtctggt ggtgccgttt
     8821 atggatcgca ttggtcgcaa gatcaatatg atggagcaag tgctcgatat cccttcccag
     8881 gaagttatct cgaaagataa cgccaacgtt accatcgacg cagtctgttt tattcaggtg
     8941 attgacgcgc cacgcgctgc ctatgaagtc agcaatctgg agctggcgat catcaacctg
     9001 accatgacta acatccgtac cgtgctgggt tcaatggaac ttgacgaaat gctctctcag
     9061 cgcgacagca tcaactcacg cctgctgcgt attgtcgatg aagccaccaa cccgtggggg
     9121 attaaagtca cccgtattga aattcgcgac gtgcgcccac cggcagagct tatctcttca
     9181 atgaacgcgc agatgaaagc ggaacgtacc aaacgcgctt acattcttga agcggaaggg
     9241 atccgtcagg cggaaatcct caaagccgaa ggtgaaaaac agtcgcaaat cctgaaagcg
     9301 gaaggcgaac gtcagtcggc gtttttacag gctgaagcgc gtgaacgttc cgctgaagca
     9361 gaagcccgcg ccaccaaaat ggtgtctgaa gccatcgcct ccggtgatat tcaggcggtg
     9421 aactacttcg tagcgcagaa atacaccgaa gcgttacagc agatcggttc gtccagtaac
     9481 agcaaagtag tgatgatgcc attagaggcc agcagcctga tggggtcgat tgccgggatt
     9541 gctgagctgg tgaaagacag cgccaacaaa cggactcagc catgatggag ttaatggtcg
     9601 ttcatccaca tattttctgg ctcagtctcg gcggtttgct gctggcagcc gagatgctgg
     9661 gcggaaatgg ttatttgttg tggagtggcg tggcagcggt gattaccggc ctggtggtct
     9721 ggctggtgcc gctgggttgg gagtggcaag gggtgatgtt tgccgtcctg acgctgctcg
     9781 ccgcctggct gtggtggaaa tggttgtcgc ggcgggtgcg cgaacaaaag cacagcgaca
     9841 gtcatttaaa ccagcgcggg cagcagctga ttggccgacg ttttgtgctg gaatctccgc
     9901 tggtcaacgg gcgcggtcat atgcgcgtcg gtgacagttc atggcctgtc agcgccagcg
     9961 aggatctcgg cgcaggtacg catgttgaag tcattgcgat agaagggata acgctgatca
    10021 tccgtgcggt catcgcctga tgcgacgctg acgcgtctta tcatgcctac aaaatatgcc
    10081 cgaatcgtag gccagataag gcgcttgcgc cgcatccggc agtcgtgcac cgacacctga
    10141 tgcgaggcgg gcgcgtatta tcatgcctgg aagtctgcgc ccgaatcgta ggccggataa
    10201 ggcgtttacg ccgcatccgg cggttatgcg ccgatgcctg atgcgacgct gacgcgtatt
    10261 atcatgccta ctaacactgc cagtctgtgc gcatccggca tgtgggcggg taatgacaat
    10321 ttactcttct gtggttttac ccataccttt ttggtcttca tccggatagt gtttttttag
    10381 atattccagg acgtttttat tgaccttgtg ttgcgtatac acccaccctt tccagtaatc
    10441 aggctggtcc aggtaaactt ctggcggaat ggtgaaatca gaaagcgtta accattcggc
    10501 taacagatcg gggtttcgtt tctggatcaa ctgcaacagc ataatcagcg acatggcaga
    10561 ggcaggagcc gtactatcgc cgcttaaata cttccacact gtcgaccggt tcgaacgaaa
    10621 aaggatcggt agcaatgccc ggtccagatt catttcatta aaaatcttct caaattcatt
    10681 catttaaatt ttcctgcctg gcgtaaacct cttaaaaatt gagatttatc
//
LOCUS       DAZGBC010000161        10592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGBC010000161 DAZGBC010000000
VERSION     DAZGBC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.162"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..1408
                     /gene="dcuC"
                     /locus_tag="ACHK1J_004105"
     CDS             41..1408
                     /gene="dcuC"
                     /locus_tag="ACHK1J_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7913308.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHKLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1450..1947)
                     /gene="sspB"
                     /locus_tag="ACHK1J_004106"
     CDS             complement(1450..1947)
                     /gene="sspB"
                     /locus_tag="ACHK1J_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO7913309.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1953..2591)
                     /gene="sspA"
                     /locus_tag="ACHK1J_004107"
     CDS             complement(1953..2591)
                     /gene="sspA"
                     /locus_tag="ACHK1J_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO7913310.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2986..3378)
                     /gene="rpsI"
                     /locus_tag="ACHK1J_004108"
     CDS             complement(2986..3378)
                     /gene="rpsI"
                     /locus_tag="ACHK1J_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO7913311.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3394..3822)
                     /gene="rplM"
                     /locus_tag="ACHK1J_004109"
     CDS             complement(3394..3822)
                     /gene="rplM"
                     /locus_tag="ACHK1J_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO7913312.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4041..5168)
                     /gene="zapE"
                     /locus_tag="ACHK1J_004110"
     CDS             complement(4041..5168)
                     /gene="zapE"
                     /locus_tag="ACHK1J_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO7913313.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKREISPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHAVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5362..5760
                     /gene="zapG"
                     /locus_tag="ACHK1J_004111"
     CDS             5362..5760
                     /gene="zapG"
                     /locus_tag="ACHK1J_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO7913314.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5914..7281
                     /gene="degQ"
                     /locus_tag="ACHK1J_004112"
     CDS             5914..7281
                     /gene="degQ"
                     /locus_tag="ACHK1J_004112"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO7913315.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7371..8438
                     /gene="degS"
                     /locus_tag="ACHK1J_004113"
     CDS             7371..8438
                     /gene="degS"
                     /locus_tag="ACHK1J_004113"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO7913316.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8501..9439)
                     /gene="mdh"
                     /locus_tag="ACHK1J_004114"
     CDS             complement(8501..9439)
                     /gene="mdh"
                     /locus_tag="ACHK1J_004114"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7913317.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9874..10344
                     /gene="argR"
                     /locus_tag="ACHK1J_004115"
     CDS             9874..10344
                     /gene="argR"
                     /locus_tag="ACHK1J_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO7913318.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2610 a         2689 c         2636 g         2657 t
ORIGIN      
        1 ttatgggatt gaccgttttc ttttgacacg gagttcaaca atgttcggca taattatatc
       61 tgtcatcgta ttaattacga tgggctattt gatcctgaaa aactacaaac ctcaggtggt
      121 gctggctgcc gcaggtatct tcctgatgat gtgcggtgtc tggttagggt tcggtggtgt
      181 actcgatccc accaaaagca gcggctactt gatcgtcgat atttataatg aaatcctgcg
      241 catgctgtcc aaccgcattg ccggattggg gctgtcgatt atggcggtgg gcggttatgc
      301 ccgctacatg gagcgcatag gggccagtcg cgcgatggtg agcttgttaa gccgcccgtt
      361 aaaactcatt cgctcgccgt atattattct gtcggcaact tacgtcatcg gccaaatcat
      421 ggcgcagttt attaccagcg cctccggtct gggtatgttg ctgatggtca ccttatttcc
      481 gacgctggtg agtctgggag taagtcgcct ctctgcggtg gcggttatcg caaccacgat
      541 gtccattgag tgggggattc tggaaacgaa ctccattttt gcagcccagg tcgcgggaat
      601 gaaaattgcc acttacttct tccactacca gcttccggtc gcctcttgcg tcattatctc
      661 ggtggcgatc tcccactttt tcgtgcaacg cgcttttgac aaaaaagata aaaatatcaa
      721 tcacgaacag gcagagcaaa aaactctcga taatgtcccg ccgctctatt acgccatttt
      781 acctgtgatg ccgttaatcc tgatgctcgg ctcgctattc cttgcccaca tcgggctgat
      841 gcagtcagaa ctgcatctgg tggtggtgat gttactgagt ttgacggtga cgatgtttgt
      901 tgagttcttc cgcaagcata aattacgcga aacaatggac gatgtgcagg cgttttttga
      961 cggcatgggt acgcagtttg ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc
     1021 gaaaggctta acgacgattg gcactgtcga tgcggttatc aggggtgcgg agcattctgg
     1081 tctgggcggt attggcgtga tgattattat ggcgctggtc attgccattt gtgccattgt
     1141 gatgggctct ggcaatgcgc cgtttatgtc atttgccagt cttattccga atatcgcagc
     1201 cggactacat gtaccagcgg ttgtaatgat tatgccgatg cattttgcca cgacgctagc
     1261 gcgcgcggtt tcgccgatta ctgcggtggt agtcgttacg tcaggaattg caggcgtttc
     1321 gccttttgcg gtggtgaagc ggacagcgat ccccatggca gtcggtttcg tggtgaatat
     1381 gattgccaca atcacgctat tttattaagc cattaaaaga caaaacaggc caccagggcc
     1441 tgttttgtat tacttaacaa cgcgtaatgc cggtcgacca ccgcgtggtg gctgcggagg
     1501 ttcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat
     1561 aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggcat cttcatcgta
     1621 ggcagcttca ggttcaaaca tcgtgccagc gccattttca cgggcgtaga tagccagcac
     1681 ggcagccagc ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac
     1741 ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg
     1801 cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccag
     1861 gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg
     1921 acgtggtgtt agctgtgaca aatccataca gattaactcc ggcccagacg catttcacgt
     1981 tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct
     2041 ttcagctctt tcgcacccgg gccgctgaac tcgatgccca gttgcggcag acgccacagc
     2101 agcggagcaa ggtagcaatc gaccaggctg aactcatcac tcaggaagta cggcttctga
     2161 ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct
     2221 tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga
     2281 tgcatgtaca gacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc
     2341 gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt cagctcacga
     2401 tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg
     2461 tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc
     2521 tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca
     2581 gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt
     2641 ggccagtcag aagttgtgtt acccagtaag gaacgactct ctttgttcga aaatcaaaca
     2701 aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc
     2761 gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat
     2821 agttgactta gccccttatt ggcgatgtgg tttttgtttt gcctgtctgt cagacggcag
     2881 caaaaagcaa ctttccattt tttacgctaa ttcaaaattt agccataaaa aaacccgccg
     2941 aagcgggttt tttcgcaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa
     3001 ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc
     3061 acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag
     3121 agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac
     3181 ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt tccagcggct gacgaactac
     3241 catgcgggca gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt
     3301 accgttgccc ggtttgatga aaacgcgagc tgcggaactt ttgcggcgac cagtgccgta
     3361 gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg
     3421 tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc
     3481 cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg
     3541 agcaatcatc tcttcaaagg tcgcttgttt gataccaccg atgtggccgg tgtggtgata
     3601 gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat
     3661 gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgcaggcg
     3721 acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca
     3781 gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa
     3841 atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt
     3901 ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa
     3961 ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt
     4021 tttgtgattt gtggcaggtt ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt
     4081 tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg ctcaccctga
     4141 taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac
     4201 tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt
     4261 acatcaaaca acatgaccgc atgaaagaga cgtgagagcg caatatagtc atgctgactg
     4321 cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt
     4381 gtcgctaatg gccgatggtt gatttctaac gttggtgaaa tttctcgttt cgcccccgcc
     4441 agcgccaacc atagtttatc catttgcgcc cggatttcat cgttgagtgg cgaaagccac
     4501 agatgcgcct gagtgagtgt acgcagtcga taatcaacac cagcgtccac gttcattaca
     4561 tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg caggccattt
     4621 cgataaagtt catccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac
     4681 agggctttca tcagaccgcc aagtagcatg gcgtcggtaa tatcagaaac aaaaaattcg
     4741 tcaaaacaga gcacgtcagt ttcggcttta aagcgatcgg caataatttc cagcggatcg
     4801 gtctgcccct gtaaggcagt tagctcttcg tgcacccgca gcataaaacg gtgaaagtgc
     4861 aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc
     4921 ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg
     4981 cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc
     5041 ctgctattga tgagttcctg ataaataatt tccaggcggc tgacggcctc tttttgaacg
     5101 tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg
     5161 ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg
     5221 aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct gtggcattaa
     5281 cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac
     5341 aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc
     5401 atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg
     5461 ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt
     5521 agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg
     5581 tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac
     5641 ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg
     5701 cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa
     5761 tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta
     5821 atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc
     5881 ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaaaccca gctgttgagt
     5941 gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt
     6001 ccaggccagg ttgccgatca ggcccccctc cccagtctgg ctccaatgct ggaaaaagtg
     6061 cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg
     6121 gaagaattca aaaagttttt tggtgatgat ttaccggatc aacctgcaca acccttcgaa
     6181 ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat
     6241 gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca
     6301 aaactgattg gtagcgatga ccagagcgat atcgccctgt tgcaaattca aaacccgagc
     6361 aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgtg
     6421 gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta
     6481 ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt
     6541 aaccgcggta actccggcgg tgcactgtta aaccttaacg gtgagttaat tggcatcaac
     6601 actgcaatcc ttgcgcctgg cggcgggagc gtcggaattg gatttgccat ccccagtaat
     6661 atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtttgtta
     6721 ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag
     6781 cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa
     6841 gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc
     6901 tctcgtatcg cgaccaccga gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc
     6961 aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa
     7021 atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc
     7081 aaaggtatta aaatcgatga agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa
     7141 aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt
     7201 aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc
     7261 atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg tgatgtccgg
     7321 ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga
     7381 agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc
     7441 cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc
     7501 ctgccagtta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc
     7561 gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa
     7621 tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga
     7681 tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt gctggtggga tctgactctc
     7741 taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc attccgatca
     7801 atgcacgtcg cgtaccgcac attggcgacg tggtactggc gatcggtaac ccgtacaacc
     7861 tcgggcagac cattacccag gggattatta gtgccacggg tcgaatcggt ctgaacccga
     7921 ccgggcggca aaacttccta caaaccgatg cttccattaa ccacggtaac tctggcggcg
     7981 cgctggtgaa ctcgctggga gaactgatgg gcattaacac gctgtcgttt gataagagta
     8041 acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa
     8101 ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggt atcggcggac
     8161 gcgagatcgc accactgcac gcgcagggcg gtggtataga tcaactgcaa gggatcgtgg
     8221 ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc aacgatctga
     8281 ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcag
     8341 aaattcgccc tggctcggtg atcccggttg tagtgatgcg tgatgataag cagttaacgc
     8401 tgcaggtcac cattcaggaa tatccggcaa ccaattaagt cgagtgctca aaacaaaaaa
     8461 ccggagtctg tgctccggtt ttttattatt cgctaatcaa ttacttatta acgaactctt
     8521 cgcccagggc gatatctttc ttgagcgtat ccagcatacc ttccagcgcg ttctgttcaa
     8581 atgcgctcag ggtaccgata gatttacgct cttccacgcc gtttttaccc agcagcagcg
     8641 gttgagagaa gaaacgggcg tactgaccgt cgccttcaac gtaggcacat tcgacaacgc
     8701 cttgttcgcc ctgcagtgcg cgaaccagag acagaccaaa acgtgcagct gcctggccca
     8761 tagacagggt tgcagacccg ccaccggctt tcgcttcaac cacctcagta cccgcgttct
     8821 ggatacgttt ggtcagatca gccacttcct gctcggtaaa actaacgcca ggaacctgtg
     8881 acagcagcgg cagaatggta acaccagagt gaccgccaat aaccggcact tcaacttcgc
     8941 ctggctgttt gcctttcagt tccgcaacaa aggtgttgga acgaatgata tccagcgtgg
     9001 taacgccaaa cagtttgttt ttgtcataaa caccggcttt tttcaacact tcagcagcaa
     9061 tcgcaactgt ggtgttaacc gggttagtga taataccaat gcacgctttc gggcaggttt
     9121 tcgcaacttg ctgtaccagg tttttcacga tgccggcgtt aacgttaaac aggtcggaac
     9181 gatccatacc cggtttacgc gctacacctg cagagataag aacgacatct gcgccttcca
     9241 gcgccggagt cgcatcttca ccagaaaaac ctttgatttt cacagcagta gggatatggc
     9301 tcagatcgac agccacaccg ggagtcactg gagcgatatc atacagagag agttctgaac
     9361 ctgaaggcag ttgggttttt aacagtagtg caagcgcctg gccaataccg ccagcagcgc
     9421 cgaggactgc gactttcatc ctaaactcct tattatattg ataaactaag atatgttgct
     9481 ccgctgccgc gaccttaatc cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt
     9541 gtagtcacgc aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaacttacg
     9601 taattaacag tcgcttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta
     9661 ccgttcagcc aaaacaacat caatttgata acaattaatt tacttttaag cagaatttgc
     9721 atgccgtgac gcaggcatgt ttctcaataa cgaaatttga taaaatcccg ctctttcata
     9781 acattatttc agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa
     9841 taatgttgta tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa
     9901 ctagttaaag catttaaagc attacttaaa gaagagaaat ttagctccca aggcgaaatc
     9961 gtcgccgcgt tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg
    10021 ttgaccaagt ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg
    10081 ccagctgaac tgggtgtacc aaccacctcc agtccattga aaaatctggt gctggatatc
    10141 gactacaacg atgcagttgt cgtgattcat accagccctg gcgcggcgca gttaattgct
    10201 cgcctgctgg actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac
    10261 accatcttta ccacccctgc taacggtttc accgtcaaag acctgtacga agcgatttta
    10321 gagctgttcg accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt
    10381 tgcttatccc tctcaacccc ctgctttccc ctgcgattaa tttaacgaat agtgcgtttt
    10441 actgcgacat gtcattcaca caatgaatac atacggtaaa aaaagcacat tatgcaaaat
    10501 tcattatcta attgaaaata ctagaattaa cgataaataa ccgtattttc aattcttttt
    10561 tgttattaaa attcacattt ttaacactta gt
//
LOCUS       DAZGBC010000162        10577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGBC010000162 DAZGBC010000000
VERSION     DAZGBC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.163"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            213..2363
                     /gene="btsT"
                     /locus_tag="ACHK1J_004116"
     CDS             213..2363
                     /gene="btsT"
                     /locus_tag="ACHK1J_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO7913319.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2413..2616
                     /locus_tag="ACHK1J_004117"
     CDS             2413..2616
                     /locus_tag="ACHK1J_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7913320.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2627..3583
                     /gene="yjiA"
                     /locus_tag="ACHK1J_004118"
     CDS             2627..3583
                     /gene="yjiA"
                     /locus_tag="ACHK1J_004118"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7913321.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILMTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3629..4543)
                     /gene="mrr"
                     /locus_tag="ACHK1J_004119"
     CDS             complement(3629..4543)
                     /gene="mrr"
                     /locus_tag="ACHK1J_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HFO7913322.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     AKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQET
                     NHLAFDFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            4731..8243
                     /gene="hsdR"
                     /locus_tag="ACHK1J_004120"
     CDS             4731..8243
                     /gene="hsdR"
                     /locus_tag="ACHK1J_004120"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HFO7913323.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIPPCENQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLD
                     DAQMCLRLGFRLAVWYYRLVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQVRE
                     KAQSQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQT
                     EQERKAYHKEITDQAIKRTLNLSEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPG
                     VNKAIAEWPTGKDETGNQGFADYVLFVGLKPIAVVEAKRKNIDVPGKLNESYRYSKCF
                     DNGFLRETLLEHYSPDEVHEAVPEYETSWQDTSGQQRFKIPFCYSTNGREYRAAMKTK
                     SGIWYRDVRDTRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQE
                     DAVRAVEKAIVKGQQEILLAMATGTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGE
                     QALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLVKRTLQSDEPMPVA
                     RYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAVKIALTATPALH
                     TVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQITTRNAQEGVYLSKGEQVERISPQGEV
                     INDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMV
                     VEELRTAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKERLPNIVVTVDLLTT
                     GVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPDVNKTSFKIFDCVDIYSTLESVDT
                     MRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLVAKLQRIIGLATFNR
                     DRSETIDKQVRRLDELCQDAAGVNFNGFASRLREKGPHWSAEVFNKLPGFIARLEKLK
                     TDINNLNDAPIFLDIDDEVVSVKSLYGDYDTPQDFLEAFDSLVQRSPNAQPALQAVIN
                     RPRDLTRKGLVELQEWFDRQHFEESSLRKAWKETRNEDIAARLIGHIRRAAVGDALKP
                     FEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRRGGKAML
                     QRTFDDNLDSLLDKFSDYIWDELA"
     gene            8320..9909
                     /gene="hsdM"
                     /locus_tag="ACHK1J_004121"
     CDS             8320..9909
                     /gene="hsdM"
                     /locus_tag="ACHK1J_004121"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HFO7913324.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPVGYRWDDLKSRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQ
                     LTELVSNMDSLDWYSGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PSTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
                     SREWIRTAKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALGELDALMRELG
                     ASDEADAQRQLLEEAFGGVKE"
     gene            9906..>10577
                     /locus_tag="ACHK1J_004122"
     CDS             9906..>10577
                     /locus_tag="ACHK1J_004122"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7913325.1"
                     /translation="MSLKNIPTNWCVTTIGDINDYKSLTFNPKSYQEDSFELYSVPSF
                     ASGSPEILLGKDIASNKQYIQFKDILLCKINPRINRVWCVKEKKEHPQIGSSEWIVIR
                     QPLIHEGFLAYFLQTQDFRDLLCSDVSGVGGSLTRAQPKRVATYNVPIAPLPEQKIIA
                     EKLDTLLAQVESTKARLEQIPQILKRFRQAVLGAAVSGKLTQNWRENNKTTPINKLLN
                     KINEER"
BASE COUNT         2627 a         2829 c         2770 g         2351 t
ORIGIN      
        1 taaataacct tcataaacct tgctcaaaac cgctcaaccg caaaactgac cgcttacgtc
       61 cctaaaataa ccactcagtt atttacctta ctttacgcgc gcgtaactct ggcagcatca
      121 ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa cggcttcggc
      181 caactattaa tcaatacatg ccaggtttta ctatggatac gaaaaaacta ttcaagcaca
      241 taccctgggt gattctcgga atcatcggtg cattctgcct cgcggtagtt gcattacgtc
      301 ggggggagca cgtcagtgcc ctgtggatcg tggtcgcctc tgtgtcggtg tatctggtgg
      361 cgtatcgcta ctacagtttg tatatcgccc agaaggtgat gaaactcgac cccacgcgcg
      421 cgacgcctgc ggttattaac aacgacggtc tgaactacgt tccgaccaac cgttacgtgt
      481 tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc gctggttggt ccggttctcg
      541 ccgcgcagat gggctacctg cctggcacac tgtggctgct ggcgggggta gtgctggccg
      601 gtgcggtaca ggactttatg gtgctgttta tctcctctcg ccgtaacggc gcatctctcg
      661 gtgagatgat caaagaagag atgggaccag taccggggac tatcgcgctg tttggctgtt
      721 tcttaatcat gatcatcatc cttgccgtcc tggcgctgat tgtggttaaa gccctggccg
      781 aaagtccgtg gggtgtcttc accgtttgct caaccgtacc aattgcgctg tttatgggta
      841 tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt ctctgtcatt ggtatcgtgc
      901 tgctggttgc ctctatctac ttcggtggcg tgattgctca cgatccgtac tggggtccgg
      961 cactgacctt taaagacacc accattacct tcgcgctgat tggctatgcg tttgtttccg
     1021 cgctgctgcc ggtgtggctg atcctcgcac cacgcgacta tctggcaacc ttcctgaaaa
     1081 tcggcgttat cgtcggcctg gcgctgggta tcgtggtgct gaacccggaa ctgaaaatgc
     1141 ctgcgatgac ccagtacatt gacggtactg gcccgctgtg gaaaggcgct ctgttcccgt
     1201 tcctgttcat caccatcgcc tgtggtgcgg tatctggctt ccacgcactg atctcttccg
     1261 gtactacgcc gaaactgctg gctaatgaaa ccgacgcgcg tttcatcggt tatggcgcaa
     1321 tgctgatgga gtccttcgtg gcgattatgg cgctggttgc agcgtccatc atcgaaccgg
     1381 gtctttactt cgcgatgaac accccgcctg ctggccttgg catcaccatg cctaacctgc
     1441 atgaaatggg tggcgagaac gcgccgatca tcatggcgca gttaaaagac gttaccgcac
     1501 acgcggcggc gaccgtcagc tcctggggct tcgtgatttc gccagagcag atcctgcaaa
     1561 ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc aggtggtgcg ccgacgctgg
     1621 cggtaggtat cgctcacgtg ttccacaaag tgctgccgat ggctgacatg ggcttctggt
     1681 atcacttcgg tattctgttc gaagccctgt tcatcctgac cgcgctggat gcgggtaccc
     1741 gttctggccg ctttatgctg caagatctgc tgggtaactt catcccgttc ctgaaaaaaa
     1801 ccgactctct ggttgccggt atcatcggta ctgcgggctg tgtgggtctg tggggctacc
     1861 tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa gagcctgtgg ccgctgttcg
     1921 gtatttccaa ccagatgctg gcagccgtag cgctggtact gggcaccgtt gtgctgatta
     1981 agatgaagcg cacccaatac atctgggtaa ctgttgttcc ggctgtatgg ctgcttatct
     2041 gcaccacctg ggcgctgggt ctgaaactgt tcagtaccaa cccgcagatg gaaggcttct
     2101 tctacatggc aagccagtac aaagagaaga ttgctaacgg tactgacctg acggcgcagc
     2161 agattgccaa catgaaccac atcgttgtga acaactacac caacgcaggc ctgagtattc
     2221 tgttcctgat tgtggtgtac agcatcatct tctacggttt caaaacctgg cttgcggtgc
     2281 gtaacagcga caaacgtact gacaaagaaa caccgtacgt tccaatcccg gaaggcggcg
     2341 tgaagatctc ttcgcaccac taaccgtgtt tagccccgct tcggcggggc tttgttctta
     2401 tcagagtgaa ctatgtttgg taacttagga caggcaaaaa aatatctcgg ccaggcggca
     2461 aagatgttga ttggcattcc agactacgac aactacgtcg agcatatgaa gaccaaccat
     2521 cccgacaagc cgtacatgag ctatgaagaa ttcttccgcg aacgccagaa cgcgcgctac
     2581 ggcggcgatg gtaaaggcgg tatgcgctgt tgttaatgga gagagcatga acccgattgc
     2641 agttacccta ctcaccggtt ttttaggcgc gggaaaaacc accctgctgc gccatattct
     2701 taacgaacaa cacggctaca agattgccgt gattgaaaac gaattcggcg aagtctccgt
     2761 tgatgatcaa ctgattggcg accgcgccac gcagatcaaa acgctgacca acggctgcat
     2821 ctgctgttcg cgctccaacg agctggaaga cgcactactc gacctgctgg ataatctcga
     2881 caagggcaat attcagttcg accgtctggt cattgaatgc accggcatgg ccgatcccgg
     2941 cccgattatt cagacctttt tctcccatga gattttatgc cagcgttacc tgctggacgg
     3001 cgtgattgcg ctggtggatg cggtacatgc cgatgagcag atgaaccagt tcaccatcgc
     3061 ccagtcccag gtgggctacg ccgaccgtat tctgatgacc aaaaccgacg tcgcaggcga
     3121 agcagaaaaa ctgcgtgaac gcctggcgcg catcaacgcc cgtgcaccgg tctacaccgt
     3181 tacccacggc gacatcgacc tgggcctgct gttcaacacc aacggtttta tgctggaaga
     3241 aaacgtcgtc agcaccaaac cgcgtttcca ctttatcgcg gataaacaaa acgatatttc
     3301 gtcgattgtg gtagaactgg attacccggt agatatcagc gaagtttccc gggtgatgga
     3361 aaacctgctg ctggaatcgg cggataaact actgcgttac aaagggatgc tatggattga
     3421 cggcgaacct aaccgcctgc tgttccaggg cgtccagcgc ctctacagcg ccgactggga
     3481 ccgcccgtgg ggcgatgaaa aaccgcatag cacgatggtg tttatcggga ttcagctgcc
     3541 ggaggaagag attcgggcgg cgtttgcggg gttgaggaag taaggggtta tgggccggat
     3601 aaggcgcagc cgcatccggc ctgatatttc actcaaaata gtccatatcc agtttcggca
     3661 ccttcaacaa acgtgaagaa acccctactt cgttttcgat cattaagtgc accaggcgtt
     3721 ccccatcaac caacaccata ccctcgacgg attgagcaaa gtcacgcgcc tgagacgtaa
     3781 atccagaagt ggtaataaac accccacgtt tcgccttttg cccagccagt gcgccgtaaa
     3841 atgcctgtaa ttctggcctg cctacagtgt tctgccaacg ttttgcctga acataaactt
     3901 tctccaggcc aagtttatca agcgatatca caccatcgat gccaccatct ccagtaccgc
     3961 caacacgctg caaatcatca cggtggccgc cataccccag gcgatgcaaa acatccagaa
     4021 caatgacttc aaagcgcgaa ggagaaacct gcaataagtt ttccagaacc tcatcagcca
     4081 ccgcatcacg aagctctttt agcgcctgat ctaaccgatc gtccgggctg ctctttgcaa
     4141 gttcttcatg atcgggagag tcggctttcg gatctaaatc gacggcatcc ggccgtgact
     4201 taagtttgac attcacaaaa tcgaaggcca gatggttcgt ctcctgctcc gtcattggct
     4261 ggggatgaga cgcaacccag tcaaaacccg caggagtcag gcaccatttg ccacgcgaca
     4321 aactttgcga caacccggca cgttttaaac ggtcatgcgc ccagcctgca cgatttttat
     4381 aaacaagttg tccgctggta atgactttcg ctcgctggct gtcatccagt cctaatgcat
     4441 ccgcggcagc ctcatgaaca tcacgcgcgg ctgcaccttc cggttttgtc gccagataac
     4501 gcagaacagg ttcaataaat ttgtcatagg taggaaccgt catagtacat ccttgcagaa
     4561 tcaggtagat gtttttcggc tactataaca ctaccaaaat agacgaacac gttagaaatg
     4621 agtcagttgc tgtgaccgtg gtcattgccc ggaaaggtac agaaagctaa gataaggcgt
     4681 tatgggcctt aaatatttgg acaggcccac acagcaatgg attaataaca atgatgaata
     4741 aatccaattt tgaattcctg aagggcgtca acgactttac ctatgccatc gcctgtgcgg
     4801 cggaaaataa ctacccggat gatcccaaca cgacgctgat taaaatgcgt atgtttggcg
     4861 aagccacagc gaaacatctt ggtctgttac tcaacatccc cccttgtgag aatcaacacg
     4921 atctcctgcg cgaactcggc aaaatcgcct tcgttgatga caacattctc tctgtattcc
     4981 acaaattacg ccgcattggt aaccaggcgg tgcacgaata tcataacgat ctcgacgatg
     5041 cccagatgtg cctgcgactc ggcttccgcc tggctgtctg gtactaccgt ctggtcacca
     5101 aagattatga cttcccggta ccggtgtttg tgttgccgga acgtggcgaa aacctctatc
     5161 accaggaagt gctgacgcta aaacaacagc ttgaacagca ggtgcgagaa aaagcgcaga
     5221 gtcaggcaga agtcgaagca caacagcaga agctggttgc cctgaacggc tatatcgcca
     5281 ttctggaagg caagcagcag gaaaccgaag cgcaaaccca ggctcgcctt gctgcactgg
     5341 aagcacagct cgccgagaag aacgcggaac tggccaagca gaccgaacag gaacgtaagg
     5401 cttaccacaa agaaattacc gatcaggcca tcaagcgtac actcaatctt agcgaagaag
     5461 agagccgctt cctgattgac gcgcaactgc gtaaagcagg ctggcaggcc gatagcaaaa
     5521 ccctgcgctt ctccaaaggc gcgcgcccgg aacccggcgt caataaagcc attgccgaat
     5581 ggccgaccgg gaaagatgaa acgggtaatc agggctttgc ggattatgtg ctgtttgtcg
     5641 gtctcaaacc catcgcggtg gtagaagcga aacgtaaaaa tatcgacgtt cccggcaagc
     5701 tcaatgagtc gtatcgctac agtaaatgtt tcgataatgg cttcctgcgg gaaaccttgc
     5761 ttgagcacta ctcaccggat gaagtgcatg aagcggtgcc ggagtatgaa actagttggc
     5821 aggacaccag cggccaacaa cggtttaaaa tccccttctg ctactcgacc aacgggcgcg
     5881 aataccgcgc agcgatgaag actaaaagtg gcatctggta tcgcgacgtg cgtgataccc
     5941 gcaatatgtc gaaagcctta cccgagtggc atcgcccgga agagttgctg gaaatgctcg
     6001 gcagcgaacc gcaaaaacag aatcagtggt ttgccgataa tcccggcatg agcgagctgg
     6061 gcctgcgtta ttatcaggaa gatgccgtcc gcgcggttga aaaggcaatc gtcaagggtc
     6121 aacaagagat cctgctggcg atggcgaccg gtaccggtaa aacccgcacc gcaattgcca
     6181 tgatgttccg cctgatccag tcccagcgtt ttaaacgcat tctcttcctt gtggatcgcc
     6241 gttctcttgg cgagcaggcg ctgggggcgt ttgaagatac gcgtattaac ggcgacactt
     6301 tcaacagcat tttcgacatt aaagggctga cggataaatt ccccgaagac agcaccaaaa
     6361 ttcacgttgc caccgtacag tcgctggtga aacgcaccct gcaatcggat gaaccgatgc
     6421 cggtagcccg ttacgactgt atcgtcgtcg atgaagcgca tcgcggctat attctcgata
     6481 aagagcagac cgaaggcgaa ctgcagttcc gcagccaact ggattacgtt tctgcctacc
     6541 gtcgtattct cgatcacttc gatgcggtaa aaattgctct caccgccacc ccggcgctgc
     6601 atacggtgca gattttcggc gagccggttt accgttatac ctaccgtacc gcggttatcg
     6661 acggttttct gatcgaccaa gatccgccga ttcaaatcac cacccgcaac gcccaggaag
     6721 gggtttatct ctccaaaggc gagcaggttg agcgcatcag cccgcagggg gaagtgatca
     6781 acgacaccct ggaagacgat caggattttg aggtcgccga ctttaaccgt ggcctggtga
     6841 tcccggcgtt taaccgcgcc gtctgtaacg aactcaccaa ttaccttgac ccgaccgggt
     6901 cacaaaaaac gctggtcttc tgcgtcacta atgctcatgc cgatatggtg gtggaagagc
     6961 tgcgtaccgc attcaagaaa aagtatccgc aactggagca cgacgcgatc atcaagatca
     7021 ctggtgatgc cgataaagac gcgcgcaaag tgcagaccat gatcacccgc ttcaataaag
     7081 agcggctgcc caatattgtg gtaaccgtcg acctgctgac gaccggcgtc gatattccgt
     7141 cgatctgtaa catcgtgttc ctgcgtaaag tacgcagccg tattctgtac gagcaaatga
     7201 aaggccgcgc cacgcgctta tgcccggacg tgaataaaac cagctttaag attttcgact
     7261 gcgtcgatat ctacagcacg ctggagagcg tcgacaccat gcgtccggtg gtggttcgcc
     7321 cgaaggtgga actgcaaacg ctggtcaacg aaattaccga ttcagaaacc tataaaatca
     7381 ccgaagcgga cggccgtagc tttgccgagc acagccatga acaactggtg gcgaagctcc
     7441 agcgcatcat cggtctggcc acgtttaacc gtgaccgcag cgaaacgata gataaacagg
     7501 tgcgtcgtct ggatgagcta tgccaggacg cggcgggcgt gaactttaac ggcttcgcct
     7561 cgcgcctgcg ggaaaaaggg ccgcactgga gcgccgaagt ctttaacaaa ctgcctggct
     7621 ttatcgcccg tctggaaaag ctgaaaaccg acatcaacaa cctgaatgat gcgccgatct
     7681 tcctcgatat cgacgatgaa gtggtgagtg taaaatcgct gtacggtgat tacgacacgc
     7741 cgcaggattt cctcgaagcc tttgactcgc tggtgcaacg ttccccgaac gcgcaaccag
     7801 cattgcaggc ggtgattaat cgcccgcgcg atctcacccg taaagggctg gtcgagctac
     7861 aggagtggtt tgaccgccag cactttgagg aatcttccct gcgcaaagcg tggaaagaga
     7921 cgcgcaatga agatatcgcc gcccggctga ttggacatat tcgccgcgct gcggtgggcg
     7981 atgcgctgaa accgtttgag gaacgtgtcg atcacgcgct gacgcgcatt aagggcgaaa
     8041 acgactggag cagcgagcaa ttaagctggc tcgatcgttt agcgcaggcg ctgaaagaga
     8101 aagtggtgct cgacgacgat gtcttcaaaa ccggcaactt ccaccgtcgc ggcgggaagg
     8161 cgatgctgca aagaaccttt gacgataatc tcgacagcct gctcgataaa ttcagcgatt
     8221 atatctggga cgagctggcc tgacacggta tcattcccgg caatttcttc ttctgcggcc
     8281 caataaattt gggccgctgc ttaatttacg gaacacccaa tgaacaataa cgatctggtc
     8341 gcgaagctgt ggaaactgtg cgacaacctg cgcgatggcg gcgtttccta tcaaaactac
     8401 gtcaatgaac tcgcctcgct gctgtttttg aaaatgtgta aagagaccgg acaggaagcg
     8461 gagtacctgc cggtaggcta ccgctgggat gacctgaaat cccgcatcgg ccaggagcag
     8521 ttgcagttct accgtaacct gctggtgcat ctgggcgccg acaatcaaaa gctggtgcag
     8581 gcggtgttcc agaacgtcaa caccaccatt acccagccaa aacagttgac cgaactggtc
     8641 agcaatatgg attcactgga ctggtacagc ggcgcgcacg gtaagtcgcg cgatgacttc
     8701 ggcgatatgt acgaaggtct gttgcagaag aacgcgaacg aaaccaagtc tggcgcgggc
     8761 cagtacttca ccccgcgtcc gctgataaaa accattattc atctgctgaa accacagccg
     8821 cgtgaagtgg tacaggaccc ggcagcaggt acggcgggct ttttgattga agccgaccgc
     8881 tacgttaagt cgcaaaccaa tgatctggac gaccttgatg gtgacacgca ggatttccag
     8941 atccaccgcg cgtttatcgg cctcgaactg gtgcctagca cccgtcgtct ggcgctgatg
     9001 aactgcctgc tgcacgatat tgaaggcaac ctcgaccacg gcggcgcaat ccgtctgggc
     9061 aacaccctgg gtagcgacgg cgaaaacctg ccgaaggcgc atattgtcgc cactaacccg
     9121 ccgtttggca gcgccgcagg caccaacatt acccgcacct ttgttcaccc gaccagcaac
     9181 aaacagttgt gctttatgca gcatattatc gaaacgctgc accccggcgg tcgtgcggcg
     9241 gtggtggtgc cggataacgt gctgtttgaa ggcggcaaag gcaccgacat ccgtcgtgac
     9301 ctgatggata agtgtcatct gcacactatt ctgcgtctgc cgaccggtat tttttacgct
     9361 cagggcgtga agaccaacgt gctgttcttt accaaaggga cggtggcgaa cccgaatcag
     9421 gataagaact gtaccgatga tgtgtgggtg tacgacctgc gtaccaatat gccgagcttt
     9481 ggtaaacgca cgccgtttac cgacgagcat ttgcagccgt ttgaacgggt gtatggcgaa
     9541 gacccgcacg gcttaagccc ccgtactgaa ggtgaatgga gttttaacgc tgaagagacg
     9601 gaagttgccg acagcgaaga gaacaaaaac accgaccagc acctggccac cagccgctgg
     9661 cgcaagttca gccgtgagtg gatccgcacc gcgaaatccg attcgctgga tatctcctgg
     9721 ctgaaagata aagacagcat tgatgccgac aacctgccgg agccggatgt attagcggca
     9781 gaagcgatgg gcgaactggt acaggcactg ggtgaactgg atgcgctgat gcgtgaactg
     9841 ggggcgagcg atgaggcgga tgcgcagcgt cagttgctgg aagaagcgtt tggtggggtg
     9901 aaggaatgag tttaaaaaat attccaacga attggtgtgt aactaccata ggggatatta
     9961 atgactataa atcattaact tttaatccca aatcatacca ggaggacagc ttcgaattgt
    10021 atagcgttcc cagctttgca tctggctcac cagaaatatt actgggaaaa gatattgcct
    10081 ccaataaaca gtatattcag tttaaagata ttttattatg caaaattaat ccccgtatta
    10141 atcgcgtttg gtgtgtaaaa gaaaaaaaag aacatcctca aatcggttca tctgaatgga
    10201 ttgtaatcag gcaaccgctc attcatgaag gttttttggc ctattttttg caaacacaag
    10261 atttcagaga tctcctatgt tctgatgttt ctggtgtggg tggttctctg acgagagcgc
    10321 aacccaaaag agtcgctaca tataatgtcc ctatagcacc tttgccagaa caaaaaatca
    10381 tcgctgaaaa actcgatacg ctgctggcgc aggtagagag caccaaagca cgtcttgagc
    10441 aaatcccaca aatcctgaaa cgttttcgtc aggcggtgct gggtgctgct gtcagcggaa
    10501 aacttacaca aaattggaga gaaaacaata aaacaacccc aattaacaaa ttattaaata
    10561 agataaatga agaaaga
//
LOCUS       DAZGBC010000163        10309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGBC010000163 DAZGBC010000000
VERSION     DAZGBC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.164"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(240..1640)
                     /gene="asnS"
                     /locus_tag="ACHK1J_004123"
     CDS             complement(240..1640)
                     /gene="asnS"
                     /locus_tag="ACHK1J_004123"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO7913326.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1808..3010)
                     /gene="pncB"
                     /locus_tag="ACHK1J_004124"
     CDS             complement(1808..3010)
                     /gene="pncB"
                     /locus_tag="ACHK1J_004124"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO7913327.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLRNFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3276..5888
                     /gene="pepN"
                     /locus_tag="ACHK1J_004125"
     CDS             3276..5888
                     /gene="pepN"
                     /locus_tag="ACHK1J_004125"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO7913328.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(5931..6698)
                     /gene="ssuB"
                     /locus_tag="ACHK1J_004126"
     CDS             complement(5931..6698)
                     /gene="ssuB"
                     /locus_tag="ACHK1J_004126"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7913329.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(6695..7486)
                     /gene="ssuC"
                     /locus_tag="ACHK1J_004127"
     CDS             complement(6695..7486)
                     /gene="ssuC"
                     /locus_tag="ACHK1J_004127"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO7913330.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7498..8643)
                     /gene="ssuD"
                     /locus_tag="ACHK1J_004128"
     CDS             complement(7498..8643)
                     /gene="ssuD"
                     /locus_tag="ACHK1J_004128"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO7913331.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQDLAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(8640..9599)
                     /gene="ssuA"
                     /locus_tag="ACHK1J_004129"
     CDS             complement(8640..9599)
                     /gene="ssuA"
                     /locus_tag="ACHK1J_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO7913332.1"
                     /translation="MRKIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLATRPY
                     AVKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9592..10167)
                     /gene="ssuE"
                     /locus_tag="ACHK1J_004130"
     CDS             complement(9592..10167)
                     /gene="ssuE"
                     /locus_tag="ACHK1J_004130"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO7913333.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
BASE COUNT         2571 a         2787 c         2643 g         2308 t
ORIGIN      
        1 ctctttcaga catccagaac gccaaaaata gtatgaaaat tgtgctatca gctaagaaaa
       61 aactaatccg caataagaat gcggattatt ttttgcgcat aaatgttgca atgtaatatt
      121 ccgtaacagg atgatcgtta tggcggggtt gcaaatagat tgcttgactt aacaaacaga
      181 taacttgaca gaaaagataa aaaaagagcc agcgggcgct ggctcttgaa gacgctaaat
      241 tagaagctgg cgttacgcgg agtacgtggg aacggaatca catcacgcac gttttgcaca
      301 ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga aacctgaatg cggaacagta
      361 ccgtagcgac gcagatcgcg ataccaccag taatcttctt tattcaggcc catttccagc
      421 atacgctcgt ccagcacgtc cagacgttct tcacgctggg agccaccaat gatctcaccg
      481 atgcccggag ccagaacgtc catagccgca acggttttac cgtcttcgtt aagacgcata
      541 tagaacgctt taatatcttt cggatagttt ttaaccacta ccggtgcttt aaagtgttct
      601 tccgccagat aacgttcatg ctcagaagag agatcaacgc cccagtaaac cgggttttca
      661 aacttcctgc cgcagttttc gagaatggtc actgcgtcgg tgtaatccac ctgcgcaaaa
      721 tcggcttcaa tgaagcgttc cagacgtgaa acggcatctt tatctacgcg ttcagcgaag
      781 aatttcatgt cgtcagcgcg ttcttcgaga accgctttga agacatattt cagcatggct
      841 tcagccagtc ccgcgatatc gttcaggtta gcaaacgcca cttccggttc cagcatccag
      901 aattccgcca ggtgacggct ggtgttggag ttttcagcac ggaaagtcgg gccgaaggta
      961 taaattttgg acaatgcgca agcgtaggtt tcgccgttca actggccaga tacggtcagg
     1021 aaagactctt taccaaagaa gtctttgtcg aaatccactt tgccctgatc gttacgcggc
     1081 aggttttcca gatccagcgt agaaacgcgg aacatttcgc cagcaccttc ggtatcagat
     1141 gcggtaatca gtggcgttga aacccagaag aatccctgct cgttaaagaa gcgatgcagc
     1201 gcctgcgcca gcgtatgacg aacgcgcgcg acggcaccaa tcaggtttgt gcgcggacgc
     1261 aggtgagcga cttcacgcag atactcaatg ctgtggcgtt ttgccgccat cgggtacgtg
     1321 tctggatctt caacccaacc agcaacttca accttgctgg cctgaatttc aaattgttgc
     1381 ccctggcccg gcgacgccac gactttaccc gtcacaatga ccgagcaacc ggtggtcaga
     1441 cgcaggacgt cttcattgta attgggcaga gaattattga tgacagcctg tacaggatca
     1501 aagcaggaac cgtcataaac ggcgaggaag gagatgccag cttttgaatc tcggcgggta
     1561 cgtacccatc cgcgtacggt gacttcgctg tcaacggcta cgcggccctg gagtacgtcg
     1621 gctacaggca caacgctcat aatattctct ctgttaatag tcggaaaaaa taaacacttg
     1681 tccacccgta aatgggggga tacctatgtt acctggcatc tgcaatcaga caagcagaat
     1741 tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa agggagccga ttagctccct
     1801 gatgatatta actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc agcgcccgaa
     1861 caaacgcttt atcgtggcag atagttttgc cagggctgtc agaaagtttc gccaccggtt
     1921 taccgttaca ctctaccaac ttaatcacaa tattcagggg ttttacctgg gggatatcgc
     1981 aggtcaggcg agtcccaata ccaaaactta attgcacgcg ggaagagaag tggcggtata
     2041 gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac cagcgtttta ctctgtggat
     2101 caattcccag cttttcataa tgtgcaatgg ctttttcacc ccattcaacc gggtcgccag
     2161 agtcatgacg caggccctga taccgactag cgaactcgac accgaaatca cgcaggaaag
     2221 catccatagt gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac tcttccagcc
     2281 aggcagcaag tgcagctcgc tggctgttgg ctagatccgg gctgatttgc tgatgtgcct
     2341 ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc gccagatcgt
     2401 agttgctggt gcccacgaac caagattcct gttgcagacg cttaacgatg gtttcttgta
     2461 cttcgcgaga aaaacggcga cgggtgccaa aatccatcag atggaagcgc gacatatcaa
     2521 gaccggcggt taacgccgag aagtcgacta atttgctttc cagcgtgtcg agggcttgcg
     2581 caacgtcggc ctgcggtgag cgatagcgat gtaccatttc actgataacc gccagcaaag
     2641 gaacttccca gaggatgact tcacgccacg ggccgcttaa acgaatatcc agcttgccat
     2701 tatcgttgga cacggtgact tgttccgggt taaagcggaa attacgcagc cagttgagat
     2761 agtcggcctt aaagaaaggc agcgcagaga gccattgata ttcatcatcc tgcaggcgca
     2821 ggtgctgcat cgcctgaacc tgttcacgaa tagcatcggc ataaataccc agcagatcgt
     2881 cacctcggca acgaaactcc gccgcgacat gcacatcgta atagtgatga aacacggctt
     2941 gctgcatatg caacttataa gcatctgtat ccagcaacga gtgcagaaca ggagaagcga
     3001 attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca ataaacatct
     3061 tcaggaactg ctggagtata ccttgtttcg caatttattg aaccccgatc acaccatatg
     3121 ccacctttct ggtcgatggc attacgcggt gtatgttata aaaatgtagc aataaaggcg
     3181 tttgtacctg aaaagatgaa gattctgcat agcgcgattt acgcaacagg aatagactga
     3241 acaccagact ctataaaaga tgctaaaggt tatttatgac tcaacagcca caagccaaat
     3301 accgtcacga ttatcgtgcg ccggattacc agattactga tattgacttg acctttgacc
     3361 tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat ggtgcatcag
     3421 atgctcccct tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat attaatgatg
     3481 agccgtggac cgcctggaaa gaagaagagg gcgcactggt catcagtaat ttgccggagc
     3541 gttttacgct taagatcatt aatgaaataa gcccggcggc gaatacggcg ctggaagggc
     3601 tttatcagtc aggcgatgcg ctttgcaccc agtgtgaagc cgaaggtttc cgccatatta
     3661 cgtattatct cgaccgcccg gacgtgctgg cgcgttttac caccaaaatt attgccgata
     3721 aaatcaaata tcccttcctg ctttccaatg gtaaccgcgt tgcgcaaggc gaactggaaa
     3781 acggacgcca ttgggtacag tggcaggacc cgttcccgaa accgtgctac ctgtttgcgc
     3841 tggtggcagg cgactttgat gtactgcgcg atacctttac cacgcgttct ggtcgcgaag
     3901 tagcactgga gctgtacgtc gatcgcggca accttgatcg cgcgccgtgg gcgatgacct
     3961 cgctgaaaaa ctccatgaaa tgggatgaag aacgctttgg cctggagtat gacctcgaca
     4021 tctatatgat cgtcgcggtg gatttcttca atatgggcgc aatggagaat aaggggctga
     4081 atatctttaa ctccaaatat gtgctggccc gcaccgatac cgccaccgac aaagattacc
     4141 tcgatattga acgcgttatc ggccatgaat atttccataa ctggaccggt aaccgagtga
     4201 cctgccgcga ctggttccag ctcagcctga aagaaggttt aaccgtcttc cgcgatcagg
     4261 aattcagctc tgacctcggt tcccgcgcgg taaaccgtat caataatgtg cgcaccatgc
     4321 gcggattgca gtttgcggaa gacgccagcc cgatggcgca cccaatccgc ccggatatgg
     4381 tcattgagat gaacaacttc tacaccctga ccgtttacga gaagggcgcg gaggtgattc
     4441 gcatgatcca caccctgctt ggcgaagaaa atttccagaa agggatgcag ctctatttcg
     4501 agcgtcatga tggtagcgca gcgacctgtg acgattttgt gcaggcgatg gaagatgcgt
     4561 cgaatgtcga tctctcccat ttccgccgtt ggtacagcca gtccggtacg ccggttgtga
     4621 ccgtcaaaga cgactacaat ccggaaaccg agcagtacac cctgaccatc agccagcgca
     4681 cgccagccac gccggatcag gcagaaaaac agccgctgca tattccgttt gccatcgaac
     4741 tgtatgataa cgaaggcaaa gtgatcccgt tgcagaaagg cggtcatccg gtgaattccg
     4801 tgctgaacgt cacccaggcg gaacagacct ttgtttttga taatgtctac ttccagccgg
     4861 tgcctgcgct gctgtgcgaa ttctctgcgc cagtgaaact ggaatataag tggagcgatc
     4921 agcaactgac cttcctgatg cgtcatgcgc gtaatgattt ctcccgttgg gatgcggcgc
     4981 aaagtttgct ggcaacctac atcaagctga acgtcgcccg tcatcagcaa gggcagccgc
     5041 tgtctctgcc ggtgcacgtt gctgatgctt tccgcgcagt gttgctcgat gaaaaaatcg
     5101 atccggcgct ggcggcagaa attctgacgc tgccttcggt aaatgaaatg gcggaactgt
     5161 tcgatatcat cgacccgatt gccattgccg aagtccgtga agcactcact cgtactctgg
     5221 cgacagaact ggcagatgag ctgctggcta tttacaacgc gaattaccag agcgagtacc
     5281 gcgttgagca tgaagatatt gcgaaacgca ctctgcgtaa tgcctgcctg cgtttcctcg
     5341 cttttggtga aacgcatctg gctgatgtac tggtgagcaa gcagtaccac gaagcgaaca
     5401 atatgactga tgcgttggcg gcgctttctg cggcggttgc tgcacagctg ccatgccgtg
     5461 acgcgctgat gcaggagtac gacgacaagt ggcatcagga cggtctggtg atggataaat
     5521 ggtttatcct gcaagccacc agcccggcgg cgaatgtgct ggagacggtg cgcggcctgt
     5581 tgcagcatcg ctcatttacc atgagcaacc cgaaccgtat tcgttcgttg attggtgcgt
     5641 ttgcgggcag caacccggca gcgttccatg ccgaagatgg gagcggttac cagttcctgg
     5701 tggaaatgct taccgacctc aacagccgta acccgcaggt ggcttcgcgc ctgattgagc
     5761 cgctgattcg cctgaaacgt tatgatgcca aacgtcagga gaaaatgcgc gcggcgctgg
     5821 aacagttgaa agggctggaa aatctctctg gcgatctgta cgagaagatc accaaagcac
     5881 tggcttaata aataaccgaa tggcggcaat agcgccgcca ttcggggatt ttacccctgt
     5941 tttctcaggc gagtttcaga ctcaccacgc tgcattaccc gttgcaacac ttccgcttcc
     6001 agctccgcta atcgcaccga ccctaaccgg cgcggacggg ggatatccac cgtcaaatcc
     6061 agaccaattt ttccctcttc aattaacaac acccggtcag ccattgctac cgcttcgctc
     6121 acatcatgcg tcaccaacag tacggtaaag ccgtgctcct gccaaagtga cacaatcaaa
     6181 tcctgcatct cgagtcgcgt taaggcatcc agcgccccga gtggttcatc aagcaacaat
     6241 aaacccggtc gatgaatcaa cgctcttgcc agcgccactc gctgtttctg cccgccagaa
     6301 agtgcggcag gccattcccc ggcgcgattc tccagtccta ccgcagccaa cgcccgacgc
     6361 gcggcatcgc gccactggcc tttaagacct aacccaacgt tatcaatcac cgatttccac
     6421 ggcagcagac gcgcatcctg aaacatcatt cgcgtgtctt cctgaatttc agccagcggt
     6481 gtggtgcccg ctaacacatc gccagcggtt ggcgtttcca gacctgccag caggcgcagc
     6541 agggtacttt tgccaccacc gctgcggccc accaccgcca caaactgacc tgccggaata
     6601 tgtaaatcca gttggttcag gacgatattt tctgcgtaat gtttgcttac tgcattgagc
     6661 aacaatggtg tgccctggtt cagacgagca gtattcatac cgtggcctcc ttcaaatgat
     6721 aagccgggtt ccagcgcaac cagaggcgct ctaacagctg cgcgctgacg tcagccagtt
     6781 tgccgagcag ggcgtaaaga ataatggcga ccaccaccac gtccgtttgc aaaaactccc
     6841 gcgcattcat cgccagataa ccaatgcctg aattggcaga aatggtttcg gcaacaatca
     6901 gcgtcagcca catcaggccc aacgcaaaac gcacgccgac cataattgag ggcagggcac
     6961 caggcaggat cacatggata aacagcggta tgccggataa tccatagcta cgcgccatct
     7021 ccaccaaccc gcgatcgata ttacggatgc catgccaggt gttgatataa atggggaata
     7081 gcgtaccgag cgccaccaga aagatttttg cggattcatc aatgccaaac cacaaaatca
     7141 ccagcgggat cagcgccaga tgcggcacgt tgcgcaacat ctgaattgag gtatccagca
     7201 gccgttctcc ccagcgagat agcccgctaa tcagtcccaa aatcaacccc agcgatccgc
     7261 caattgaaaa gccaatcagc gcccgccagg agctgatcgc cagatgctgc cacagttcgc
     7321 cgctggcgga gagcgtccag aacgccgtca ccactccttc cggtgaaggc aaaatacgcg
     7381 tcgacagcca gccaaccgag gaggccagtt gccacaccgc cacgatgccc accggtaaaa
     7441 accagggggc aacgcgcaat aaccacttct tcactggcgt tgccatcatc gtgttcctta
     7501 gctttgcgcg actttacggg ggataaaatc attcgccacc gcttcgcctt gcggattcag
     7561 cggctgcggc tggggaattt ccggaatggc gacatccaga tgtgggaaca gcaactcgcc
     7621 gacccgatac gcttcttcca gatgcggata gcccgaaagc acaaagctgt cgatgccaag
     7681 cgcggcatat tcgttgattc gcgcagcgac cgtaggacca tcgcccacca gcgccgtccc
     7741 ggcaccgccg cgcactaagc caacgcccgc ccataaattg gggctgatct ccagattgtc
     7801 gcgcttgcca ttatgcaacg ccgccattcg ctgttgcccc acggaatccg tgcgggcaaa
     7861 cgcggcctgt gctttggcga tagtctcatc atcaagatgc gaaattaacc gttcggcggc
     7921 ctgccacgct tcgtcgttgg tttcgcgaac aatcacatgc agacgaatac cgaaacgaat
     7981 tttgcgtcca tgcgcggccg ctttcgcccg cacttgttcg attttctctt ttaccagctc
     8041 tggcggttcg ccccaggtga gataaagatc aacttgttcg gcggccagat cctgagcgac
     8101 gtctgaagat ccgccaaagt aaagcggggg ataaggctgt tgaatcggcg gaaagagcag
     8161 tttggctcca cgcacatgaa tatgtttgcc gttgaaatcg acggtttcgc ccagcaataa
     8221 acgccgccag acctgggtaa attccgccga ggcttcgtag cgctcgctat gatcaaggaa
     8281 cacgccatcg cctgccagct cttgtggatc gctgcctgtg accaggttaa ataacgcacg
     8341 tccgttagag agacggtcaa gcgtggcggc ctggcgggcg gcaacggtag gagacgtgac
     8401 actgggacgc agagcgacaa gaaacttcag ccgctgcgtc accgggatca tcgacgctgc
     8461 aaccagccac gcatcttcgc aggagcgccc cgttgggatc agcacaccgg tatagcccag
     8521 acgatccgcc gcttgcgcaa tttgttgcag ataaccgtga tcaaccgggc gtgaaccttc
     8581 ttccgttccc agataatgcc cgtcaccgtg ggtcggtaaa aaccagaaca tattcagact
     8641 cataattgtt ttccttccag ttgagtgggc tgccagatgc gctggcgaat atcgactttt
     8701 tttggcacca gacggttttc ataaaacaga tctgccgttt gctgttgtaa ggcagcaact
     8761 tctgcgctca ccggcttgat agtggtaggg ggacgatgat ctaaataaga ggcaatcacc
     8821 ggcgccggta agcccatcgt ttttgccagt aaagcgatac tttgctcgcg ctgactgcgg
     8881 gttaacgcat cggcttcgct aaaggttgcc agtacgccct gaataaatgc gccgtttttt
     8941 actgcatacg gacgggttgc cagataaaac gatccggttt gattgaggtc ggtaccgtct
     9001 ttcagcaccc gcacgccgcc ctgtaataac gcggcggagt agtagggatc ccagatagcc
     9061 caggcatcga cgttaccctg ctggaatgcg gcgcgggcat cagctggcgt cagataagtg
     9121 ggctggatgt cagtaaactt aagtccggcc tggcgtagtg cgcgcagtaa aagattgtgt
     9181 gaactggaac ctttctggaa cgcaactttg tggcctttaa gatcggctac ggttttgatc
     9241 gggctgtttt ctgccaccag aatcacctcg gcttttggct tcggcggctc gacgcccaca
     9301 tataccaaat cagccccggc agcctgggca aagattggcg gaatatcccc ggtactgccg
     9361 agatcaatac tgccaacgtt taacgcttct aacatttgcg gacccgcggg gaactccacc
     9421 caggagattt ttgattgcgg atagcgtttt tccagtaact ggtggctttt tgccaatacc
     9481 ataccaatac tgcctttctg atagcctata cgtaacgctt caggcgagga ttctgcagca
     9541 accacaatcg tagagacgct aagcaatccc accagcgcca gtttaatgat cttacgcatg
     9601 ggcattacct cgcagagaat gaagatcagg aacctgaaca tcgcggcggt gcaatgcctg
     9661 ccagaaagtt tctagcgcgg tgtcaagacg ggtttgtaaa ttcggcgtga actggggttt
     9721 gtgatggtaa tcaattactt gtgagtcatc ggcaaacacg ccgtgcagga tctcctgggc
     9781 tttcagcgca cttaaaactg gcttaagggc ataatcgacc gccagcagat gggccaccgt
     9841 accgcccgtc gccagcggta gcaccacttt gccttgcaac gcacgttctg gcagcaggtc
     9901 gagcagggtt ttcagcgcac cggaataagc ggctttatac acaggcgtgg cgacaatcag
     9961 cccgtcagcc tgttgcaact gttcggtgaa ggtatggagt gccggactat cgaaacgagc
    10021 ataaagcaga tcttccgcgg cgaagttttg cagattccag tgataaacct caacatccag
    10081 gccatttagt ttttcccgcg catattccag caaggagctg gagcgagaag gaaagcgagg
    10141 actacccgcc agggtgatga cacgcatact ctctccttat aaccaattgt tcttgttttg
    10201 ttaacattga taacaatttg ctcagtctgt cggagagaca agaaaatgcc aaatataaat
    10261 tttgtgtatc tttttctgaa aataaactaa agaaagggct ttatatatc
//
LOCUS       DAZGBC010000164        10299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGBC010000164 DAZGBC010000000
VERSION     DAZGBC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.165"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..456
                     /locus_tag="ACHK1J_004131"
     CDS             <1..456
                     /locus_tag="ACHK1J_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7913334.1"
                     /translation="PVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     NTNVLEIAIKTRIKLFWRPAAVKIIPEQAIKNVKWSRQGNNLIVENPNPIHINVMSVT
                     VDDKDVSLNMIRPFETLTLPIPAGVAGKSLVWRFINDYGAISQPLKKNL"
     gene            478..3012
                     /locus_tag="ACHK1J_004132"
     CDS             478..3012
                     /locus_tag="ACHK1J_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7913335.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFVQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYGDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSTEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSSSNYHDDNNDNFGKEQIF
                     MFSMSIPLSGWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLIQMDAICR"
     gene            3024..4115
                     /locus_tag="ACHK1J_004133"
     CDS             3024..4115
                     /locus_tag="ACHK1J_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913336.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGVCFNSDGATHMSNLSFGPLT
                     VAAANNHSGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLE
                     RTLNGLNYYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNL
                     DSGRSAKLSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQN
                     CELTPNQSIDVDFQKINSGEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASM
                     IAANRSADATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFY
                     SAPASLTGTKPAPDNGFTATATLVIEFTN"
     gene            4143..5225
                     /locus_tag="ACHK1J_004134"
     CDS             4143..5225
                     /locus_tag="ACHK1J_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913337.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGDERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIIGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            5437..6477
                     /gene="pstS"
                     /locus_tag="ACHK1J_004135"
     CDS             5437..6477
                     /gene="pstS"
                     /locus_tag="ACHK1J_004135"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO7913338.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6564..7523
                     /gene="pstC"
                     /locus_tag="ACHK1J_004136"
     CDS             6564..7523
                     /gene="pstC"
                     /locus_tag="ACHK1J_004136"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO7913339.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7523..8413
                     /gene="pstA"
                     /locus_tag="ACHK1J_004137"
     CDS             7523..8413
                     /gene="pstA"
                     /locus_tag="ACHK1J_004137"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO7913340.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8584..9357
                     /gene="pstB"
                     /locus_tag="ACHK1J_004138"
     CDS             8584..9357
                     /gene="pstB"
                     /locus_tag="ACHK1J_004138"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO7913341.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9372..10097
                     /gene="phoU"
                     /locus_tag="ACHK1J_004139"
     CDS             9372..10097
                     /gene="phoU"
                     /locus_tag="ACHK1J_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO7913342.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         2631 a         2454 c         2609 g         2605 t
ORIGIN      
        1 cccgtttctc gcattgaacc gcagcaagag aaaatattac gcattttaaa aacggctggc
       61 gagttaccaa aagatcgaga gtccgtattc tggttgaacg tgaaaaacat ccctccttca
      121 attgataaaa aaaataccaa tgttcttgag atagccataa aaacaagaat taagttattc
      181 tggcgaccag ccgcggtaaa gataatccct gagcaggcta ttaaaaatgt taaatggtcg
      241 cggcagggta ataatctcat cgtcgaaaat ccaaatccga tccatattaa tgtaatgagt
      301 gtgacggtgg acgataaaga cgtgtcgcta aatatgatcc gcccttttga aacattgaca
      361 ttaccaattc cggctggcgt ggcaggaaaa tcgctcgttt ggcgctttat taatgattac
      421 ggtgcaatta gtcaaccact aaagaaaaac ctttaagcgc cggataagga tatatcgatg
      481 gcctcttcgc atcttttcat tacgcttgca tcgggcatat gtctgctctg ttccatatct
      541 gcttttgccc gggatagctt gttcaacccc agattactgg aactggatca tcctgcggat
      601 aatattgata ttcaccagtt caaccgttcg aataccttac ctgcgggaac atacaaagtt
      661 gatgtgatga tcaacggcat gctcttcgaa cgccaggaag ttaaatttgt ccaggataac
      721 cctgatgctg aactccaccc atgctacgtg gcgataaaaa acgtactggc gacctatggt
      781 ataaaagttg atgcgataaa atctctggcg aatgttgatg acaaaacatg cgtaaatcca
      841 gttccgctga tcgacggggc tacctggtta ctggacgcca gtaaacttgc attgaatatt
      901 actattccgc aaatttatct caacaatgca gttaatggtt atatcagccc ttcccgttgg
      961 gatcagggga tcaatgccat gatgatgaat tatgattttt cggcatcgca taccatccgg
     1021 tcaaattatg acgacgacga tgacagttat tatctgaatt tgcgtaatgg tattaattta
     1081 ggcgcatggc gttttcgtaa ttacagcacc ctgaattctt atgacggtaa tgtggactac
     1141 cattccgtca gtaattacat tcagcgcgac atcatggcat tacgtagcca gattatgatt
     1201 ggcgatacct ggacggcaag cgatgtattt gatagtacac aggtgcgtgg cgtgcggctg
     1261 tataccgatg acgatatgtt gccctccagc cagaatggct ttgcgccagt ggtacatggg
     1321 attgcgaaaa ctaacgccac ggtgatcatc aaacaaaacg gctacgttat ttatcaatca
     1381 gccgtaccac agggcgcatt tgccctcacc gacttaaaca cgaccagtag cggcggcgat
     1441 ctcgatgtca ctatcaaaga agaagatggc agcgagcagc actttattca gccatttact
     1501 tcactggcca ttctcaagcg tgaaggtcag accgatgtag accttagcat tggagaagtg
     1561 cgcgacgaaa gcggctttac gcctgaggtc ttgcagttac aagcaatgca cggtttccct
     1621 ttgggaataa ccttgtatgg cggaacacaa ctggcaaatg attacgcttc tgccgcgctg
     1681 ggtattggta aagatatggg ggcgctgggc gcgatttctt ttgacgtgac tcatgcccgc
     1741 tcgcagtttg actacggcga taatgagagt ggtcaatcgt atcgttttct ctattccaaa
     1801 cgttttgaag acaccaatac cacctttcgt ctggtgggtt atcgctactc tacggagggg
     1861 ttctacaccc tcaatgaatg ggtgtcgcga caggataacg attctgattt ctgggtaacg
     1921 ggcaaccgtc gcagccgctt cgaaggcacc tggacgcaat ctttcacgcc aggctggggc
     1981 aatatttatt taacattcag tcgacaggaa tactggcaga ccgatgaggt cgagcgttta
     2041 ttacagttcg gctataacaa caactggcga aacatctcct ggaacgtttc ctggaactat
     2101 acggactcga tcaagcgctc atcgagcaac tatcatgatg ataacaatga taatttcggc
     2161 aaagaacaga ttttcatgtt ctcaatgtcg ataccgctat cgggctggat ggaagacagc
     2221 tacgtcaact attcgttaac gcaaaacaac caccatgaaa gcacgatgca ggtcggtctg
     2281 aacggaacga tgctggaagg gcgtaacctg tcttataacg tacaggaatc gtggatgcac
     2341 tctcctgatg actcctacag cggcaatgcc ggaatgacct atgacgggac ttatggctcg
     2401 gtcaatggca gctattcctg gagccgtgac tcccaacatt ttgattatgg cgccagaggc
     2461 ggcgtgctgg tgcatagtga cggagtgacc ttctcgcagg aactgggcga aacggtggca
     2521 ttagtcaaag cgccgggcgc agaaggcctg tccattgaaa acgccaccgg gatttctacc
     2581 gactggcgtg gttataccgt aaaaacgcag cttagcccat atgacgaaaa ccgcgtggca
     2641 ttgaacagcg actatttctc caaagccaat attgaactgg aaaacaccgt catcaacctg
     2701 gtaccaacgc gcggtgcggt ggtgaaagcc gaatttgtca cccatgtcgg ttatcgcgtg
     2761 ctatttaacg tccgccaggt caacggtaaa ccaataatgt ttggcgcgat ggcaaccgcc
     2821 tctctcgaaa cgggcacagt caccgggatt gtcggtgata acggcgaact gtatctctcc
     2881 gggatgcctg aaaaaggcga gtttttattg agttggggac aagctgcgga tgaaaaatgt
     2941 aaggcggcct atcacatcac ccataaacct gatgatacca gcctgattca aatggatgcg
     3001 atttgtcgct aaaggaagca tgaatgaaaa aaatattatc agggttgatt ctgctgcttt
     3061 gctgtcctta tggtttcgcc gctaatggtg atggcgtttg ttttaatagt gatggcgcaa
     3121 cgcacatgtc aaatttatca tttggtccgc tgacggtggc tgcggcgaat aaccactccg
     3181 gatacaatat tttcgaggca ctgagcaaca cgactggaac atacccggtg cgctgtcact
     3241 gtgatgacac gcatggcgga ccgggccaac aaacagcatt ttttcctatc ttctacacgg
     3301 gggatgccgc accggggctt gtgcttgagc gcactcttaa tgggttaaat tactatgctc
     3361 tgaatgatta tttatcggtc ggcgtgacga tttttattat taataaccaa tatgcggcca
     3421 ttccttttga acacttatcc aaccaatcca cctcaccgca acatacctgt ggagcaggta
     3481 ataatgggag cactgtaaat ctggattcag ggcgctcggc aaaattatct ttctatgttc
     3541 ggcattctat tactggcacg gtgacaatac ccacaacgga agtcgcctgg ttatacgcgg
     3601 gcatgtccga tcattttccc aaaacgaccc ccgtttctaa agtgacaatt cgcggacaat
     3661 taacggcccc gcagaactgt gagttaacgc caaatcagag catcgatgtc gattttcaaa
     3721 aaattaatag cggtgagttc tcctcaacgg cgggttcaat tattgcagaa cgaaagatta
     3781 aaaccgaagt cacagtatcc tgtaccggga tggaagacgt aaggtctacg gaggtggtga
     3841 gtgcgtcgat gattgcggca aacagaagtg ccgatgccac catgatcgtg acgagtaatc
     3901 cggatgtggg aattaagatt tttgataaga acgaccgtcc agtgaatgtg gatgggggca
     3961 acttacccgc tgatatgggt gctattagtc gattaggaaa aaccgatggt agcgtaacgt
     4021 tttattcagc gcccgccagt ctgacgggca caaaaccagc gcctgataat ggatttaccg
     4081 ccacagccac gctggttatt gaatttacta actaatcttg tgttagtaaa aggaataatt
     4141 tcatgaaaaa atggcattat atattttgca taattctctt tcatttaggg ttgccgtgcg
     4201 ggtatgcggc aaatgatggc acgtgtgcaa caagaggcgg cacacataca ttaagcctta
     4261 attttcctct gacaacggtc agtgcagcaa acaatgtgcc tggaaataca ttaatagata
     4321 ttgctaatgc aacatcttct gaaaattata gcgttctgtg taactgtgat tcaaaacata
     4381 gcaatggcgc ttatcacgaa atatattata ccgcagaccc tgctcccggt atggtttata
     4441 gcaccaccgc aagtggtctt gctttttact atcttaacga atatgtcgat gtgggaacaa
     4501 aaatatctgt gctaaatgcg gggtatacgg cagttccttt tgaacatgtt tccaaccagg
     4561 caactacaac agatcacact tgtcagggaa acaaaactac agcggttggc gtgagcctga
     4621 aaactggagc agatgcgaag atttcatttc gtattaaacg ttcaataaat ggaacggtag
     4681 taatacctat caccgatatt gcattgctgt atgccaacat atccagcacc acgacccgtg
     4741 gtgaggcgat tgcaaaagtt cgaatttcag gcagtttgac cgcaccacag tcttgtcaga
     4801 taaatgcagg acaggtgatt tattttgatt ttgatactat tcctgcgtcc gaattttcat
     4861 ctaccgccgg gcaagccatt acttcacgaa aaatcactaa aacagtgagt attgagtgta
     4921 cggggatggg ggatgagcgt acgcagaaag tcgatgcttc ttttacgggg acgaaccgaa
     4981 gcagtgacga tacgatggtg gcgacagaca atgctgatgt cgggatcaaa atttacaata
     5041 aatcgaatgc tgaagttagc gtcaacaacg gcaagttacc cgcagacatg ggcaacacga
     5101 ccattattgg tcgtaaaaat ggttcggtaa ctttttcggc agcacctgcc agctttaccg
     5161 gtgcccggcc tcagcccggc gtttttaacg ctaccgcgac cttaaccatt gaatttgtaa
     5221 actaaccttt tgcggctgtt ttatgacagc cgttcatatt tcctctcctt tcatgaaaaa
     5281 atgctgatat ttttcccctt caatatcaat aagtaataac tttcctttgg gtgtcatcaa
     5341 actgtcacat tccttacata taactgtcac ctgtttgtcc tattttgctt ctcgtagcca
     5401 acaaacaatg ctttatgaat cctcccagga gacattatga aagttatgcg taccaccgtc
     5461 gcaactgttg tcgccgcgac cttatcgatg agtgctttct ctgtgtttgc agaagcaagc
     5521 ctgacaggtg caggtgcaac cttccctgcg ccggtgtatg ccaaatgggc tgacacttac
     5581 cagaaagaaa ccggtaataa agttaattac cagggtatcg gttcttccgg tggcgtaaaa
     5641 cagattatcg ccaataccgt tgattttggt gcttctgacg cgccgctgtc tgacgaaaaa
     5701 ctggctcagg aaggtctgtt ccagttcccg accgtgattg gcggcgtggt gctggcggtt
     5761 aacattcctg ggttgaagtc tggcgagctg gtgctggatg gtaaaaccct cggcgacatc
     5821 tacctcggca aaatcaagaa gtgggatgat gaagccatcg ccaaactgaa tccgggtctg
     5881 aaactgcctt cacagaacat tgctgtagta cgccgcgcag atggctccgg gacttccttc
     5941 gtcttcacca gctacctggc gaaagtgaat gaagagtgga aaaacaacgt tggtactggc
     6001 tctaccgtaa aatggccgat cggtctgggt ggtaaaggta acgacggtat cgctgcgttc
     6061 gttcagcgtc tgccgggtgc aattggttac gttgaatatg cttatgcgaa gcaaaacaac
     6121 ctggcgtaca ccaaactgat ctccgctgat ggtaaacctg ttttgccgac tgaagaaaac
     6181 ttcgctaatg cagcaaaagg tgcagactgg agcaaaacct tcgctcagga tctgaccaac
     6241 cagaaaggcg aagatgcgtg gcctattacc tctaccacgt tcattctgat ccacaaagat
     6301 cagaagaaac cagaacaagg cacagaagtg ctgaaattct tcgactgggc gtacaaaacc
     6361 ggggctaaac aggcgaacga cctggattac gccagcctgc cggatagcgt agttgaacag
     6421 gttcgcgctg cgtggaagac caatattaaa gacagtagcg gcaagccgct gtactaataa
     6481 aactccaggc cggataaggc attaaacggc ttatccggca ttacaaaatg actttgtaaa
     6541 cgcgtttaac tgaagagtaa cttatggctg caaccaagcc tgcttttaac ccaccgggta
     6601 aaaagggcga cataattttc agcgtgctgg taaaactggc ggcgctgatt gtgctattga
     6661 tgttgggtgg cattattgtc tctctgatca tctcctcatg gccgagcatt cagaaatttg
     6721 gtctggcttt cctgtggacc aaagagtggg atgcgccgaa cgatatctac ggggcgctgg
     6781 tgccgatcta cggtacgttg gtgacttcgt ttatcgcgct gctgatcgcc gtcccggtga
     6841 gtttcggtat cgccctgttc ctgactgagc ttgcgcctgg ctggctgaaa cgcccgctgg
     6901 gtatcgccat tgagctgctg gcagccattc caagtatcgt ttacggcatg tggggcctgt
     6961 ttatctttgc gccgctgttc gccgtttact tccaggagcc ggtcggcaat atcatgtcga
     7021 atatcccgat tgtcggcgcg ctgttctctg gcccggcatt tggtatcggt atcctcgcgg
     7081 caggcgtgat cctcgccatc atgattattc cgtacattgc ggcggtaatg cgcgatgtgt
     7141 tcgaacaaac cccggtgatg atgaaagagt cggcctacgg tattggctgc accacctggg
     7201 aggttatctg gcgtatcgtt cttccgttca ccaaaaatgg tgttatcggc ggcattatgc
     7261 tggggctggg ccgcgcgctt ggtgaaacca tggcggtgac ctttatcatc ggtaacacct
     7321 accagctcga cagcgcctcg ctgtacatgc cgggcaacag tatcacctct gcgctggcga
     7381 acgaatttgc ggaagcggaa tccggtctgc acgttgccgc actgatggaa ctgggcctga
     7441 tcctgtttgt gattaccttc atcgtcctcg ccgcatcgaa gtttatgatt atgcgcctgg
     7501 ctaagaatga gggggcacgc taatggctat ggttgaaatg caaaccactg cggcgcttgc
     7561 tgaatcccgc cgcaaaatgc aggcgcgtcg ccgcctcaag aaccgtattg cactgacgct
     7621 ctcaatggcg acgatggcct tcggtctgtt ctggctgatc tggattttaa tgtccaccat
     7681 cactcgcggt atcgatggta tgtcgctggc tctgttcact gaaatgacgc cgccgcccaa
     7741 tacggaaggt ggtggtctgg cgaacgctct ggcgggtagc gggctgttaa ttttgtgggc
     7801 cacggtattc ggtacgccgc tgggcattat ggcggggatt tatctggctg aatatggtcg
     7861 taaatcctgg ctggcagaag tgattcgctt cattaacgac attctgctct ctgcgccgtc
     7921 gattgtggtt ggtctgtttg tttacaccat cgtggtggcg cagatggagc acttctccgg
     7981 ttgggcgggt gtgattgcgc tggcgctgtt gcaggtaccg attgttatcc gcaccaccga
     8041 gaacatgctg aaactggtgc cggacagcct gcgtgaagcg gcttatgcac tgggtacgcc
     8101 gaagtggaag atgatctccg cgattacgct gaaagcgtcg gtgtccggaa ttatgaccgg
     8161 tatcctgctg gcgattgccc gtattgccgg tgaaaccgcg ccgctgctgt ttaccgcgct
     8221 ctccaaccag ttctggagca cggacatgat gcagccgatc gccaacctgc cggtgacgat
     8281 ctttaagttt gcgatgagcc cgtttgcgga atggcagcaa ctggcctggg ccggggtatt
     8341 gatcattacc ctgtgcgtac tgctgctgaa cattctggcg cgcgttgttt tcgcgaagaa
     8401 taaacacggt tgatattgct gacacggttt tcccctctcc ccagaggggc gaggggaccg
     8461 accgagcgcc tttttgactc tgtacacggt taacactttg ccggatgcgg cgtaaacgcc
     8521 tgatccggcc tacggtaagc ctgattagcg aagtgcatca ggcacgatga ggaaaagatt
     8581 gcaatgagta tggttgaaac tgccccgagt aaaattcagg ttcgtaattt gaacttctac
     8641 tacggcaaat tccatgccct gaaaaacatc aacctggata tcgctaaaaa ccaggtaacg
     8701 gcgtttatcg ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt caacaaaatg
     8761 tttgaactgt atccggaaca gcgtgcggaa ggtgaaattc tgcttgatgg cgacaacatc
     8821 ctgaccaact ctcaggatat cgccctgctg cgtgcgaaag tgggcatggt gttccagaag
     8881 cctacgccgt tcccgatgtc catttacgac aacatcgcct ttggtgtccg tctgtttgag
     8941 aagctctccc gtgccgacat ggacgagcgc gtacagtggg cattgaccaa agccgcattg
     9001 tggaacgaaa ccaaagataa attgcaccag agcggttact ctctctctgg tggtcagcaa
     9061 caacgtctgt gcattgcgcg tggtatcgcc attcgcccgg aagtgctgct gctcgacgaa
     9121 ccgtgttcgg cgctcgaccc tatctctacc gggcgtattg aagagctgat caccgaactg
     9181 aagcaggatt acaccgtggt gatcgtcacc cacaacatgc agcaggctgc gcgttgttcc
     9241 gaccacacgg cgtttatgta cctgggcgaa ttgattgagt tcagcaacac ggacgatctg
     9301 ttcaccaagc cagcgaagaa acaaacagaa gactacatca ccggtcgtta cggttgattc
     9361 aggagtgcgt tatggacagt ctcaatctta ataaacatat ttccggccag ttcaacgccg
     9421 aactggaaag tatccgcacg caggtgatga ccatgggcgg catggtggag cagcagcttt
     9481 ctgatgcaat caccgcgatg cacaaccagg acagcgatct ggcgaagcgc gtcatcgaag
     9541 gcgacaagaa cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg cgcattattg
     9601 ccaaacgtca gccgacagcg agcgacctgc gtctggttat ggtgatcagt aaaaccattg
     9661 ctgaactgga gcgtattggc gacgtggcgg acaaaatctg ccgtactgcg ctggagaaat
     9721 tctcccagca gcatcagccg ttgctggtaa gtctggagtc gctgggccgt cataccatcc
     9781 agatgctgca cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa gcggtacgta
     9841 tttatcgtga agataaaaaa gtcgatcagg agtatgaagg tattgttcgt caactgatga
     9901 cctacatgat ggaagattcg cgtaccattc cgagcgtact taccgcgcta ttctgtgcgc
     9961 gttctatcga acgtattggc gaccgctgcc agaatatttg tgagtttatc ttctactacg
    10021 tgaaggggca ggatttccgt cacgtcggtg gtgatgagct ggataaactg ctggcgggaa
    10081 aagaccccaa agagtagtgc taactgtagg ccagataaag ctttttgccg catctggcaa
    10141 tcagcccggt catacatagt taccgggctg ataaactttc tcactgaaat attgccacac
    10201 aaactcacct cgaaactctc cccagtacat tgcatataca ttattacatt tgaattattt
    10261 tacttttaat gtagtgccac gctataaaaa catttttgt
//
LOCUS       DAZGBC010000165        10259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGBC010000165 DAZGBC010000000
VERSION     DAZGBC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.166"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..762
                     /gene="yahL"
                     /locus_tag="ACHK1J_004140"
     CDS             <1..762
                     /gene="yahL"
                     /locus_tag="ACHK1J_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO7913343.1"
                     /translation="ILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDDLV
                     DDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFRILENQKG
                     TVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEHHMPLLRLFPKG
                     KECMHVEKWLREMSSIPETDAPIKQVLAHGLLLHLKNIYPVFPESLVMLLLSVLDVKT
                     YRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1177..1422
                     /gene="yahM"
                     /locus_tag="ACHK1J_004141"
     CDS             1177..1422
                     /gene="yahM"
                     /locus_tag="ACHK1J_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO7913344.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAWIRQIRLIT"
     gene            complement(1439..2110)
                     /locus_tag="ACHK1J_004142"
     CDS             complement(1439..2110)
                     /locus_tag="ACHK1J_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO7913345.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2257..2532
                     /gene="yahO"
                     /locus_tag="ACHK1J_004143"
     CDS             2257..2532
                     /gene="yahO"
                     /locus_tag="ACHK1J_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO7913346.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(2633..4219)
                     /gene="prpR"
                     /locus_tag="ACHK1J_004144"
     CDS             complement(2633..4219)
                     /gene="prpR"
                     /locus_tag="ACHK1J_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO7913347.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLRYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            4458..5348
                     /gene="prpB"
                     /locus_tag="ACHK1J_004145"
     CDS             4458..5348
                     /gene="prpB"
                     /locus_tag="ACHK1J_004145"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO7913348.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARNQVK"
     gene            5508..6677
                     /gene="prpC"
                     /locus_tag="ACHK1J_004146"
     CDS             5508..6677
                     /gene="prpC"
                     /locus_tag="ACHK1J_004146"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO7913349.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSAIGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6711..8162
                     /gene="prpD"
                     /locus_tag="ACHK1J_004147"
     CDS             6711..8162
                     /gene="prpD"
                     /locus_tag="ACHK1J_004147"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO7913350.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAVTLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            8202..10088
                     /gene="prpE"
                     /locus_tag="ACHK1J_004148"
     CDS             8202..10088
                     /gene="prpE"
                     /locus_tag="ACHK1J_004148"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO7913351.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         2509 a         2632 c         2757 g         2361 t
ORIGIN      
        1 atacttaata cggttaaaaa caatcagtta ccagaggata tcaaaagctc cctggtttcc
       61 tgtgtcgata tattcaaggt tttgattaaa caatattatg attatcctta tgattgtcgt
      121 gatgatttag tcgatgacga caaactcatt catctcatgg ctgccgtacg agactgtgag
      181 tggtcagacg ataacgcact caccataaat gtgcagttta atgatttccc tggattttat
      241 gactggatgg attaccctga tcacccggtt aagtttgttt ttcgcatact tgaaaatcaa
      301 aaagggacag tttgggtata tgaccaggat gatgcgtttc ttgatattaa agcaaacgtg
      361 caggctggac gctttaccgg gcttaagaaa ttagtgcagt ttatcgattc agtgcgaaca
      421 gattgcaaat gtattcttct tgaacaccat atgcctttgc tgagactttt ccctaaaggg
      481 aaagagtgta tgcatgtgga aaaatggctt cgcgaaatgt cgtctattcc ggagacagac
      541 gcgcccatta agcaggtgct tgcgcatgga ttattattac acttaaaaaa tatctaccct
      601 gtttttcctg aatcactggt gatgttattg ctctctgttc tggacgtaaa aacatacaga
      661 gatgacgcac gattaaatga atggatatct aatagagtgc aggaattagg tgacagatat
      721 tatcctgtaa ataagcacgt taaaatccgc tacactcttt agttcagaaa gggttccacc
      781 tgcaagttgt tattccacct gagagtgaat cgcaatgcag gtaatcattt catctgtata
      841 tctgtatgcc aactggcatt ccatctcttt tttcgccgat attttgttgt gatcggcgac
      901 acttcgctta aaaaagcacc agtagtggtt tcgcagccat gcggtgtata aaaaaaatga
      961 tctcatgcag atgttttgtg aatgtgtggg ttgacattca tatgaaaaaa atcataattc
     1021 catcatgttt gtgtggggtc ttttctgtat cttacgcatc gcactcaagc ctgacagaaa
     1081 atatgctgta aggctcatat caaaaaccgc cattagctca tcaggaagag cagacgacaa
     1141 ccaggattgt tgtatggtac ggggttcgag gcctcgatgg cggtccaact tttcaaaact
     1201 cttctgaatc agatcccctt attatcatcc ctgcaaagtg gaacattgcc attgtttggc
     1261 tattcaggac gggtcagacc aatgaaaaaa gcccacacag gggagagtgg gctgaaatgg
     1321 gaagctaaag actcaagtaa acttatcgga aataaggacc acgcattacg ggggctatca
     1381 tcgccgatgg cctggattcg gcaaatcagg ttgattacgt agagagtaaa ttatctgctc
     1441 accgctgcgt caccccttcg taaatcaggc gtagcgcgaa tacaccaata attgcaccaa
     1501 taacccgact ggcaacgcgt tgcatacgcc cataagcacg acgcacagcg ggcaaagaaa
     1561 acgcctgact aagaaagact cgccagataa ctgatgcgag cacaatcccc gcccaggcca
     1621 ttaaacgtgc ccatgttggt gtttcggcat ttaatgttac tgagaaaata ctgataaaaa
     1681 ataaaacggt ttgcgggtta gagagatcgg taattaatcc gcggcgaaaa aagacatacc
     1741 agggggcgct aatcggttgt tgtagtgtgc tcatttgcgg tgttgactgg cggcgcatgc
     1801 tgcaccacgc aaaccataag agataagcgc cgccgacgat tctgataagc gaaaaaatct
     1861 cctcacactg cgtaattagt gttgcaagac caaacaaacc caacccggaa taaaatgcat
     1921 cgcccagcgc cacgcccagc ccggtcagca cccctgcgcg tcgaccggaa gccaggctgg
     1981 tttgtactac cacaaagaga ttggctcccg gattaaaaaa agtaatcacg aacagtccca
     2041 cggtcaggta aacggcatgc aaaggatcca tagtgatttc atccataaat aagtgaacta
     2101 actgcatcat atttctacca aaaataatcg ggtgcgagag agatcacaaa gtgtcttatt
     2161 tccggttact ggcgtttatg ccctgactga actaattatt aatcaaccca ataatgtggg
     2221 tgggtgatag tgtgataaca actctggagc cgtaatatga aaataatctc taaaatgtta
     2281 gtcggtgcgt tagcgtttgc cgttaccaat gtctatgccg ctgaattgat gaccaaagcg
     2341 gaatttgaaa aagttgaatc gcagtatgaa aaaataggtg atatttcaac cagtaatgaa
     2401 atgtcgactg cagatgcaaa agaagattta atcaaaaaag ccgatgaaaa aggggctgat
     2461 gtgttggtgc tgacctctgg tcaaactgac aataagatcc acggcacggc ggatatttat
     2521 aagaagaagt aattctgaac cctatgtaaa catccccgat gcgtaagttt atcggtgatc
     2581 atcatctatt gaaatttatg ccggataaag cgttcgcgct gcattcggca gttcagcttt
     2641 tcagccgccg ccagaacgtc gtccgactga tgcctaaata attcgccgct gctgttttat
     2701 cgccattaaa tttctccagt gcctgttgtg gtgtcagtaa gcgtggagcg ggagttttcg
     2761 ccgactcgcg cgccagttcc ggcagtagca gctgcaaaaa ttgcggcgtt aaatccggcg
     2821 tcggttccac acttaaaaat agcgccagtc gctccatcat attgcgcagt tcacgaatat
     2881 tgcccggcca gtcgtagcgc agcagcacag tttcacttgc ctgtaacccc tggcgtaatg
     2941 cagcagaaaa cggggcggag agcgccgcca gagacacttt caaaaaactt tccgccagtg
     3001 gcagaatatc tgccacccgc tcgcgcagtg gtggcaattg cagacgcaaa atactcaacc
     3061 gataaaacag atcacggcga aactgccctt gccgcatatc ttcttccaga ttgcagtgag
     3121 tggcgctaat gacccgcaca tccaccggaa caggctgatg cccgccgacg cgagtaacct
     3181 ctttttcttc cagcacccgt aacaggcgag tctgcaaagg tagcggcatt tcgccaatct
     3241 catccaaaaa cagcgtgcca ccgtgggcga tttcgaacag tccggcgcga cctccgcgtc
     3301 gcgagccggt aaacgcccct tcctcatagc caaacagctc cgcttccagc agcgattcgg
     3361 caatcgcccc gcagttgacg gcaacaaacg gatgcgactt tttgccctgt cgcgcatcgt
     3421 ggcgggcaaa atattcccga tgaattgcct gggccgccag ctctttgccc gtccccgttt
     3481 ccccctcaat caacaccgcc gcactggagc gggcatacag caaaatagtc tgccgtactt
     3541 gttccatctg tggtgattga ccgagcatat cgcccagcac gtaacgagta cgcagggcgt
     3601 tacgggtagc atcgtgagtg ttatggcgta acgacatgcg cgtcatatcc agcgcatcgc
     3661 tgaacgcctg gcgcacggtg gcggcggaat agataaaaat tccggtcatt ccggcttctt
     3721 ctgccagatc ggtaatcagc cccgcgccga ccaccgcttc ggtgccgtta gcttttagct
     3781 cgttaatctg cccgcgtgcg tcttcttcgg taatgtagct acgttgatcg aggcgcaaat
     3841 taaaggtttt ttgaaacgcc accagtgccg gaatagtttc ctgataagtg acaacgccga
     3901 tagaagaggt gagctttccg gcttttgcca gtgcttgtaa cacatcgtag ccgctcggtt
     3961 taatcaaaat aactggcact gacaggcggc ttttcaggta cgcgccgtta gagccagcag
     4021 cgatgatggc gtcacagcgt tcgtttgcca gtttcttgcg gatgtaggtc actgcttttt
     4081 caaagccaag ctgaataggg gtaatgttcg ccaggtgatc aaactcgagg ctgatatcgc
     4141 gaaacagctc gaacaggcgc gttacagata ccgtccagat aaccggtttg tcgtcattca
     4201 gccgtggtgg atgtgccata gcgcaccgca aagttaagaa accgaatatt gggtttagcc
     4261 ttgtttcata attgttgcaa tgaaacgcgg tgaaacattg cctgaaacgt taactgaaac
     4321 gcatatttgc ggattagttc atgactttat ctctaacaaa ttgaaattaa agatttaatt
     4381 ttattaaggc aattgtggca caccccttgc tttgtcttta tcaacgcaaa taacaagttg
     4441 ataacaaagg atgggctatg tctctacact ctccaggtaa agcgtttcgc gctgcactgt
     4501 ctaaagaaaa tccattgcag attgttggca ccattaacgc caaccatgcg ctgttggcgc
     4561 agcgtgccgg atatcaggca atttatctct ccggcggtgg cgtggcggca gggtcgctgg
     4621 ggctgcccga tctcggtatt tctacccttg atgatgtgct gaccgacatt cgccgtatca
     4681 ccgacgtttg ttcgctgccg ctgctggtgg atgcggatat cggttttggt tcttcggcct
     4741 ttaacgtggc gcgtacggtg aaatcgatga ttaaagccgg tgcggcagga ttgcatattg
     4801 aagatcaggt tggtgcgaaa cgctgcggtc atcgtccgaa taaagcgatc gtctcgaaag
     4861 aggagatggt ggatcggatc cgcgcggcgg tggatgcgaa aaccgatcct gattttgtga
     4921 tcatggcgcg caccgatgcg ctggcggtag aggggctgga tgcggcgatc gagcgtgcgc
     4981 aggcctatgt tgaagcgggt gccgagatgt tgttcccgga ggcgattacc gaactcgcca
     5041 tgtaccgcca gtttgccgat gcggtgcagg tgccaatcct cgccaacatt accgaatttg
     5101 gcgccacgcc gctgtttacc accgacgaat tacgcagcgc tcatgtcgcg atggcgctgt
     5161 acccgctttc agcgtttcgc gccatgaacc gcgccgccga acatgtctac aacgtcctgc
     5221 gccaggaagg cacgcagaaa agcgtcatcg acaccatgca gacccgcaac gagctgtacg
     5281 aaagcatcaa ctactaccag tacgaagaga agctcgacga cctgtttgcc cgtaaccagg
     5341 tgaaataata aacgcccgtt ggttgtattc gacaaccgat gcctgatgcg ccgctgacgc
     5401 gacttatcag gcctacgggt gtactgaact gtaggtcgga taagacgcat ggcgtcgcat
     5461 ccgacaacaa tctcgaccct acaaatgata acaatgacga ggacaacatg agcgacacaa
     5521 cgatcctgca aaacagtacc catgtcatta aaccgaaaaa atcagtggcg ctttctggcg
     5581 ttccggcggg caatacggcg ctctgcaccg tgggtaaaag tggcaatgac ttgcattacc
     5641 gtggttacga tattcttgat ctggcggaac attgcgaatt tgaagaagtg gcgcatctgc
     5701 tgatccacgg caaactgccg acccgtgacg aactcgccgc ctacaaaaca aaattgaaag
     5761 ccctgcgcgg tttaccggct aacgtgcgta ctgtgctgga agccttacca gcggcgtcgc
     5821 atccgatgga tgtcatgcgc accggcgttt ctgcgatcgg ctgcacgctg ccagaaaaag
     5881 aggggcacac cgtttccggt gcgcgggata ttgccgacaa actgctggca tcacttagct
     5941 cgattctcct ttattggtat cactacagcc ataacggcga acgcatccag ccggaaaccg
     6001 atgacgactc catcggcggt cacttcctgc atctgctgca cggcgaaaag ccgtcgcaaa
     6061 gctgggaaaa ggcgatgcat atttcgctgg tgctgtacgc cgaacacgag tttaacgcct
     6121 ccacctttac cagtcgggtg attgcgggca ctggctctga tatgtattcc gcgattattg
     6181 gcgcgattgg cgcactgcgc gggccgaaac acggcggggc gaatgaagtg tcgctggaga
     6241 tccagcaacg ctacgaaacg ccagacgaag ccgaagccga tatccgcaag cgggtggaaa
     6301 acaaagaagt ggtcattggt tttgggcatc cggtttatac catcgccgac ccgcgtcatc
     6361 aggtgattaa acgtgtggcc aagcagctct cgcaggaagg cggctcgctg aagatgtaca
     6421 acatcgccga tcgcctggaa acggtgatgt gggagagcaa aaagatgttc cccaatctcg
     6481 actggttctc cgctgtttcc tacaacatga tgggcgttcc caccgagatg ttcacaccac
     6541 tgtttgttat cgctcgcgtc accggctggg cggcgcacat tatcgaacaa cgtcaggaca
     6601 acaaaattat ccgtccttcc gccaattatg ttggaccgga agaccgccag tttgtcgcgc
     6661 tggataagcg ccagtaaacc tctacgaata acaataagga aacgtaccca atgtcagctc
     6721 aaatcaacaa catccgcccg gaatttgatc gtgaaatcgt tgatatcgtc gattacgtca
     6781 tgaactacga aatcagctct aaagtggcct acgacaccgc acattactgc ctgctcgaca
     6841 cgctcggctg cggtctggaa gctctcgaat acccagcctg taaaaaactg ctggggccaa
     6901 ttgtccccgg caccgtcgta cctaacggcg tgcgcgtccc cggaactcag ttccagctcg
     6961 accccgtcca ggcggcattt aacatcggcg cgatgatccg ttggctcgat ttcaacgata
     7021 cctggctggc ggcggagtgg gggcatcctt ccgacaacct cggcggcatt ctggcaacgg
     7081 cggactggct ttcgcgcaac gcggtcgcca gcggcaaagc gccgttgacc atgaaacagg
     7141 tgctgaccgg aatgattaaa gcccatgaaa ttcagggctg catcgcgctg gaaaactcct
     7201 ttaaccgcgt cggcctcgac cacgttctgt tagtgaaagt ggcttccacc gccgtggtcg
     7261 ccgaaatgct tggcctgacc cgcgaggaaa ttctcaacgc tgtttcgctg gcgtgggtgg
     7321 acggtcagtc gctgcgcacc tatcgccatg cgccgaacac cggcacgcgt aaatcctggg
     7381 cggcgggcga tgccacttcc cgcgcggtac gtctggcact gatggcgaaa acgggcgaaa
     7441 tgggttaccc gtcagccctg actgcgccgg tatggggctt ctacgacgtc tcctttaaag
     7501 gtgaatcgtt ccgcttccag cgtccgtacg gttcttacgt catggagaat gtgctgttca
     7561 aaatctcctt cccggcggag ttccactccc agacggcagt tgaagcggcg atgacgctct
     7621 atgaacagat gcaggcagca ggcaaaacgg cggcggatat cgaaaaagtg accattcgca
     7681 cccacgaagc ctgtattcgc atcatcgaca aaaaagggcc gctcaataac ccggctgacc
     7741 gcgaccactg cattcaatac atggtggcga tcccgctgtt gttcgggcgc ttaacggcgg
     7801 cagattacga ggacaacgtt gcgcaagata aacgcatcga cgccctgcgc gagaagatca
     7861 attgctttga agatccgacg tttaccgctg actaccacga cccggaaaaa cgcgccatcg
     7921 ccaatgccgt tactcttgag ttcactgacg gtacgcgctt tgaagaagtg gtggtggagt
     7981 accccattgg tcatgctcgc cgccgtcagg atggcattcc gaagctggta gataaattca
     8041 aaatcaatct cgcgcgccag ttcccgactc gccagcagca gcgcattctg gaggtttctc
     8101 tcgacagagc tcgcctggaa cagatgccgg tcaatgagta tctcgacctg tacgtcattt
     8161 aagtaaacgg cggtaaggcg taagttcaac aggagagcat tatgtctttt agcgaatttt
     8221 atcagcgttc gattaacgaa ccggagcagt tctgggccga gcaggcccgg cgtattgact
     8281 ggcagacgcc ctttacgcaa acgctcgatc acagcaatcc gccgtttgcc cgttggtttt
     8341 gtgaaggccg aaccaacttg tgtcacaacg ctatcgaccg ctggctggag aaacagccag
     8401 aggcgctggc attgattgcc gtctcttcgg aaacagaaga agagcgcacc tttacctttc
     8461 gccagctgca tgacgaagtg aacgcggtgg cgtcaatgct gcgctcactg ggcgtgcagc
     8521 gtggcgatcg ggtgctggtg tatatgccga tgattgccga agcgcatatt accctgctgg
     8581 cctgcgcgcg cattggcgct attcactcgg tggtgtttgg gggattcgcc tcgcacagtg
     8641 tggcggcgcg aattgatgac gctaaaccgg tgctgattgt ctcggcagat gccggagcgc
     8701 gcggtggcaa aatcattccc tataaaaaat tgctcgacga tgcgataagt caggcgcagc
     8761 atcagccgcg ccatgttttg ctggtagatc gtgggctggc gaaaatggcg cgcgttagcg
     8821 ggcgggatgt cgatttcgcg tcgttgcgcc atcagcacat cggcgcgcgg gtgccggtgg
     8881 cctggctgga atcaaacgaa acctcctgca ttctctacac ctccggcacg accggcaaac
     8941 ctaaaggcgt gcagcgtgac gtcggcggat atgcggtggc gctggcgacc tcgatggaca
     9001 ccatttttgg cggcaaagcg ggaggtgtgt tcttttgtgc ttcggatatc ggctgggtgg
     9061 tggggcattc gtatatcgtc tacgcgccgc tgctggcggg gatggcgact atcgtttacg
     9121 aaggattacc gacctggccg gactgcggcg tgtggtggaa aatcgtcgag aaatatcagg
     9181 ttagccggat gttctcagcg ccgaccgcca ttcgcgtgct gaaaaaattc cctaccgctg
     9241 aaattcgcaa acacgatctc tcgtcgctgg aagtgctcta tctggctgga gaaccgctgg
     9301 acgagccgac cgccagttgg gtgagcaata cgctggatgt gccggtcatc gacaactact
     9361 ggcagaccga atccggctgg ccgattatgg cgattgctcg cggtctggac gacaggccga
     9421 cgcgtctggg aagccccggc gtgccgatgt atggctataa cgtgcagttg cttaatgaag
     9481 tcaccggcga accgtgtggc gtcaacgaga aagggatgct ggtggtggaa gggccgctgc
     9541 cgccggggtg tattcagacc atctggggcg acgacgaccg ctttgtgaag acgtactggt
     9601 cgctgttttc ccgcccggtg tacgccacct ttgactgggg catccgcgat gctgacggtt
     9661 atcactttat tctcgggcgc actgacgatg taattaacgt tgccgggcat cggctgggga
     9721 cgcgcgagat tgaagagagt atctccagtc atccgggcgt tgccgaagtg gcggtggttg
     9781 gggtgaaaga tgcgctgaaa gggcaggtgg cggtggcgtt tgtcattccg aaagagagcg
     9841 acagtctgga agatcgtgat gtggcgcatt cgcaagagaa ggcgattatg gcgctggtgg
     9901 acagccagat tggcaacttt ggccgcccgg cgcacgtctg gtttgtctcg caattgccaa
     9961 aaacgcgatc cggaaaaatg ctgcgccgca cgatccaggc gatttgcgaa ggacgcgatc
    10021 cgggggatct gacgaccatt gatgatccgg cgtcgttgga tcagatccgc caggcgatgg
    10081 aagagtaagg tattgtcgga tgcgtcgcgt ggcgcatccg acagttgtgc gcagatgcct
    10141 gatgcgacgc tggcgcgtct tatcatgcct acaggcctga accgtaggtc ggataaggcg
    10201 ctcgcaccgc atccgacacc gtgctcagtg cctgatgcga cgcaggcgcg tcttatcat
//
LOCUS       DAZGBC010000166        10256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGBC010000166 DAZGBC010000000
VERSION     DAZGBC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.167"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            167..1849
                     /gene="ydeN"
                     /locus_tag="ACHK1J_004149"
     CDS             167..1849
                     /gene="ydeN"
                     /locus_tag="ACHK1J_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7913352.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1901..3058
                     /gene="ydeM"
                     /locus_tag="ACHK1J_004150"
     CDS             1901..3058
                     /gene="ydeM"
                     /locus_tag="ACHK1J_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7913353.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFTKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEVFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3024..3146)
                     /locus_tag="ACHK1J_004151"
     CDS             complement(3024..3146)
                     /locus_tag="ACHK1J_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913354.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
     gene            3348..5033
                     /gene="yddA"
                     /locus_tag="ACHK1J_004152"
     CDS             3348..5033
                     /gene="yddA"
                     /locus_tag="ACHK1J_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO7913355.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            5071..7443
                     /gene="yddB"
                     /locus_tag="ACHK1J_004153"
     CDS             5071..7443
                     /gene="yddB"
                     /locus_tag="ACHK1J_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO7913356.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSNVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDLFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDTWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7500..>10256
                     /gene="pqqL"
                     /locus_tag="ACHK1J_004154"
     CDS             7500..>10256
                     /gene="pqqL"
                     /locus_tag="ACHK1J_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7913357.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLMALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNT"
BASE COUNT         3010 a         2277 c         2250 g         2719 t
ORIGIN      
        1 gaaattactt agtagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt
       61 tttagctaaa tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct
      121 tcgtaaaatt actttggaat taagtacaat aagaagagga acatttatga agtctgcatt
      181 aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt
      241 tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc
      301 agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg tactgaccat
      361 ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat
      421 ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc
      481 gcaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg
      541 ctatgtggcg cacggtgttt ccggcccctc ccgcgccgca ataatgaccg gtcgagcgcc
      601 cgcccgcttt ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac
      661 tttcttgcct gaattattcc aaaatcatgg ttattacact gcagcagtag gtaaatggca
      721 cttgtcaaaa atcagtaatg tgccggtacc ggaagacaaa caaacacgtg actatcatga
      781 caacttcacc acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt
      841 tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg
      901 tgaacgcgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt
      961 tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc
     1021 gcacctgcca aatgataatc ctgcaccgga gcaatatcag aagcaattta ataccggtag
     1081 tcaaacggca gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat
     1141 tctcgaacaa ctgaagaaaa acggacagta tgacaatact attattctct ttacctccga
     1201 taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag
     1261 tcagacctat cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca
     1321 acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc
     1381 agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt
     1441 gcaagataag aaacaaggcg agccacataa aaatctgacc tggataacct cttattctca
     1501 ctggtttgat gaggaaaata ttccattctg ggataactac cacaaatttg tccgtcatca
     1561 gtcagacgat tacccgcata accccaacac tgaggactta agccaattct cttatacggt
     1621 gagaaataac gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa
     1681 actgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga
     1741 gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaagtaaa
     1801 tcaggagaag tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca
     1861 tgcggcggaa ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc
     1921 ctccagtttt caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca
     1981 gtttactcat gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat
     2041 cgcagcgtct ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg
     2101 cctggatttt ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat
     2161 ttttaatgca ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa
     2221 agaacatgaa tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta
     2281 cagacgcagt aattcaggta acggtacttt tacaaaagtg atagcagcca tcgagcgtct
     2341 gaaatcatat caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta
     2401 ccctcttgag gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga
     2461 attgctagaa accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg
     2521 tatcattgat ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat
     2581 gcaatgggtt aaaaacgatg tgggtgaagt tttcatccgt cagtttgaaa gctttgtcag
     2641 ccgttttttg gggaatgggc ataccagttg tattttccag gagtcctgca aggataatct
     2701 ggttgttgaa agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa
     2761 aattggaaac attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca
     2821 aaaaaaacgg atttcagcga aatgtctgaa atgtgcatat aaacctatct gcaatggcgg
     2881 ttgtcctaag catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg
     2941 ttataaaatc cttttttcaa ccatggtacc ttatatgaac gccatggtag agttagctaa
     3001 gaacagagta ccgctttatc acattatgga tgttgcaaaa caaatggaga ataattaata
     3061 acacgccctc ttagctccag tcttagccta cgtcttatga ccttaacaaa gcacccgaca
     3121 taaattcctg tcgggtgtgt cgtcatcatc atcttacctt gcgaaaatcc tggcatacgt
     3181 ggtatgcgtc agggcattag caataccctt gattatcaat agcataacca taaccactga
     3241 aattaaaaat ttctatatat ttcatattgt taaatgatac ttaaataaat ttcatcagta
     3301 aatcattgcc atagataatc attggatggc acaatcaatt ttcgctaatg ataaccattc
     3361 ccattacgtt ttgtatgttg atagcaaagt atctctgtct gctgaagccc ttttggctgc
     3421 gtaagaataa taaaacttca gtgttgttaa tcattattat cctcgccatg atccttggcg
     3481 tggttaaaat tcaggtctgg ttaaacgact ggaataatga ttttttcaat gcgctaagcc
     3541 agaaagaaac cgataagctt tggcagcttg tcctttggtt ccctgccctg ctggggatct
     3601 ttgtattaat atccgtaaac aaaacatggt taattaaact gctgactatt cgctggcgcg
     3661 aatggttaac agattactac cttaatcggt ggtttgcaga taaaaactac tacttcacgc
     3721 agatctacgg cgagcataaa aatacagata accctgacca acgtatcgct gaagacattc
     3781 tcttactgat cagcaaaacg ctcagtcttt catttggctt catccagtca cttagcatgc
     3841 tgatcacctt taccgttatt ctttggcaaa gcgcgggtac gctctcattc actgttggtg
     3901 gaacagaatg gaatatccag gggtatatgg tctataccgt cgtgctcatt gtgatcggtg
     3961 gaactttatt tacccataag gtgggtaaac gtattcgtcc acttaatgtg gaaaaacaac
     4021 gtagcgaagc gacatttcga actaatcttg tgcagcataa taaacaggca gaactgattg
     4081 cgctaagcaa tgctgagtct ctacaacgcc aggagttgag cgacaatttt cataccatta
     4141 aagagaactg gcatcgttta atgaatcgtc agcggtggct tgattactgg cagaatattt
     4201 attcgcgttc gcttagcgtt cttccgtact ttctgttatt gccgcagttt attagcggac
     4261 agatcaatct gggcggactg atgaaatcgc gccaggcatt tatgctggta tcgaacaatt
     4321 taagctggtt tatttataaa tatgacgaac ttgctgaact ggctgcggtt atcgatcgct
     4381 tgtatgagtt ccatcaactc accgaacagc gccctacgaa taagcctaaa aattgccaac
     4441 atgcggtaca agtggctgac gcgagtattc gtacccctga taataagatc atattagaga
     4501 acctgaactt tcatgttccg ccaggcaaat ggctattact gaaaggttac tctggtgcgg
     4561 gaaaaaccac actgcttaaa acattatccc actgctggcc gtggtttaaa ggtgatattt
     4621 cttctcctgc tgacagttgg tatgtgtcac aaacaccgtt aatcaaaacc ggcttactga
     4681 aagagattat ttgtaaagca cttcccctgc ccgtagacga taaatcgttg agcgaagtac
     4741 tgcatcaggt tggtcttggg aaattggctg cgcgtattca tgaccacgat cgctggggag
     4801 atattctttc cagcggcgaa aaacaacgta tcgccctggc acgattaatt ttacgacgtc
     4861 cgaaatggat atttcttgac gaaactacct ctcatcttga ggaacaagag gctatccgct
     4921 tactgcgttt agtgcgtgaa aaactaccca caagcggcgt cattatggtt acacatcaac
     4981 ccggtgtctg gaacctggcc gatgatattt gtgacattag cgcggtttta taaaaacaat
     5041 aaaagccctg ctgtacggag atataaataa atgaagcgag ttcttattcc tggcgtcatt
     5101 ttatgtggcg ctgatgtggc gcaggccgtc gatgataaaa acatgtacat gcattttttt
     5161 gaagagatga cggtctatgc tcctgtccct gtacccgtaa acggcaacac gcattacacc
     5221 agtgaaagca tcgagcgttt accgaccggg aatggcaata tcagcgatct gctgagaacc
     5281 aaccctgcgg tacgcatgga ttcaacgcaa agtacctcgt tgaaccaggg agatattcgc
     5341 ccggagaaaa tctctattca cggtgcgtcg ccctaccaga atgcctattt gattgatggt
     5401 attagtgcca ctaataacct gaacccagcg aatgagtccg atgccagtag tgcaaccaat
     5461 attagcggga tgtcacaggg gtattatctt gatgtcagct tactggacaa tgtgacgctt
     5521 tatgacagtt ttgtgccggt tgaattcggt cgcttcaatg gcggggtaat tgatgcaaag
     5581 atcaaacgct tcaacgctga tgatagcaac gtgaaactgg gttatcgcac tacgcgttcg
     5641 gactggttaa catcgcatat cgatgagaat aacaagagcg catttaatca aggttcttca
     5701 ggaagtactt attactctcc agattttaaa aagaactttt ataccttgtc gtttaatcag
     5761 gaactcgctg ataactttgg cgttaccgcc ggtttatcgc gccgccagtc tgatatcacc
     5821 cgcgcggatt atgtttcaaa tgacggcatt gtcgccggtc gggcacagta taaaaacgtt
     5881 atcgatactg cattgagcaa atttacctgg tttgccagcg acctctttac ccacgattta
     5941 accttaaaat ataccggctc cagccgtgat tataatacca gcaccttccc gcagtctgat
     6001 cgcgaaatgg gtaataaatc ctatggtctg gcatgggata tggatacgca gctcgcatgg
     6061 gccaaactac gtaccaccgt tggttgggat catattagtg attatacccg tcacgatcat
     6121 gacacctggt acaccgaact ttcatgtaca tatggtgata ttacagggcg ttgcacccgt
     6181 ggcggattag gacacatttc ccaggctgta gataattaca ccttcaaaac acgcctggac
     6241 tggcaaaaat tcgccgtggg taatgtttcg catcaaccct acttcggcgc ggaatacatc
     6301 tattccgatg catggactga acgccataac cagtctgaat cctatgtgat taatgctgcc
     6361 ggaaagaaaa ctaaccatac catttaccat aaaggtaaag gcagcctggg aattgacaac
     6421 tacacactgt atatggcgga tcgcattagc tggcgtaatg tgtcattaat gcccggcgtg
     6481 cggtatgact atgacaacta tctgtcaaac cacaatatct ccccgcgctt tatgacggaa
     6541 tgggatattt ttgctgatca aacctcaatg attaccgcag gttataaccg ttactatggt
     6601 gggaatattc ttgatatggg attacgtgat atccgcaata gctggacgga atcggtatca
     6661 ggcaataaaa ccctgacgcg ttatcaggat ttgaaaacgc cttataacga tgaactggca
     6721 atgggattgc agcagaaaat cggtaagaac gttattgcgc gtgcaaacta tgtttaccgt
     6781 gaagcgcatg atcaaatcag caaaagcagt cgtaccgaca gcgcgactaa aaccactatt
     6841 actgaatata acaacgatgg caaaaccaaa acgcattcgt tcaacctcag ttttgagctg
     6901 gccgaacccc tgcatatccg ccaggtagat attaacccac aaattgtctt tagctatatc
     6961 aagagcaagg gcaacttgtc gttaaacaat ggttatgagg agagcaatac cggtgataac
     7021 caggtggttt ataacggtaa tcttgtctct tacgatagcg ttccagtggc agattttaat
     7081 aacccattaa agatctcctt aaacatggat ttcacgcatc aaccgagcgg gttggtgtgg
     7141 gcgaatacgc tggcctggca agaagcgcgt aaagctcgca ttatcctggg taagacgaat
     7201 gcgcaataca tcagcgaata ttcagattac aagcagtatg ttgacgaaaa actggatagc
     7261 agcctgacct gggacacccg cttgtcctgg acgccacaat ttctgaaaca acaaaacctg
     7321 acgatcagtg ccgatattct caatgtactg gatagcaaaa ccgcggttga tacaacgaat
     7381 accggtgtgg cgacctacgc cagtggccgt actttctggc ttgatgtcag catgaaattt
     7441 taatagttcg gattttactt aaccgggtaa attcgcccgg ttttcgcatg gagataatta
     7501 tgagaaacct ctgtttctta ctgacgttag tggcaactct gttgctcccc gggcggctga
     7561 ttgccgccgc cttaccgcag gatgaaaagt taattaccgg gcaattggac aatggcttgc
     7621 gatatatgat ttatccgcat gctcatccaa aggatcaggt aaatttatgg ctgcaaattc
     7681 ataccggttc attgcaggaa gaagacaatg agcgcggcgt ggctcatttt gtagaacata
     7741 tgatgtttaa cggcacaaaa acatggccgg gtaataaagt catcgaaaca tttgagtcaa
     7801 tgggcctgcg ttttggtcgc gatgttaatg cctataccag ctatgacgaa acggtgtatc
     7861 aggtgagttt gccaactacg cagaaacaaa atctgcaaca agtgatggca atcttcagtg
     7921 aatggagtaa tgccgcaacc tttgaaaaac tcgaagtaga cgctgaacgc ggcgtaatta
     7981 ctgaggaatg gcgtgcccat caggatgcga aatggcgcac ctctcaggcg cgccgccctt
     8041 tcctgctggc taatacccgt aatttagacc gtgaacctat cggcctgatg gatactgtcg
     8101 ccacggtcac accggcacaa ttgcgccaat tttatcaacg ctggtatcaa ccaaataata
     8161 tgacctttat cgtggttggc gatatcgaca gtaaagaagc gctggcgctg ataaaggata
     8221 atttaagtaa gcttccggct aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg
     8281 aaaaccacct gcgctttaat atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac
     8341 tctattatcg cctgccaatg gtacaagtga acgatgagca aagctttatc gaacaagctg
     8401 aatggagcat gttagttcag ctgttcaatc aacgtctgca ggaacgtata cagtcgggcg
     8461 agttgaagac tatttctggc ggcactgcgc gcagcgttaa aattgcaccc gattatcagt
     8521 cgctgttttt ccgtgtaaat gcacgagacg ataatatgca ggatgctgcg aatgcattaa
     8581 tggcagagtt ggcaaccatt gatcagcatg gtttttctgc tgaagaactc gatgatgtca
     8641 aatctacccg cctcacctgg ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac
     8701 gtatgctgac cagtcgcctg gcatccagct cattaaataa tacgccgttc ttgtcgccgg
     8761 aagagacata tcaactttcg aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg
     8821 aaaaatggca gcagttaaga aagaaccagg acgcattttg ggagcaaatg gtaaacaatg
     8881 aggttgccgc aaaaaaagca ttgtctcctg cggctatcct ggcgctggaa aaagagtacg
     8941 ccaacaaaaa gctggcggct tacatcttcc caggcagaaa tttatcgtta acagtagacg
     9001 ctgacccaca ggcggaaatt agcagcaaag aaacgctggc tgagaatctg acatcattaa
     9061 cactttccaa tggtgccagg gttattctgg caaaatccgc gggtgaagag caaaagctac
     9121 aaattattgc cgtatcgaat aaaggcgatt taagtttccc tgcgcagcaa aaatcactta
     9181 tcgcgctggc aaataaagca gttagcggaa gcggcgttgg cgaactctcc tcttccagcc
     9241 tgaaacgctg gagtgcggaa aactcggtaa ccatgagcag taaagtcagt ggcatgaata
     9301 cgttgctctc cgttagcgcg cggactaata accctgaacc tggtttccag ttgattaacc
     9361 agcgaatcac ccacagcacg attaacgaca atatttgggc atcgctacaa aatgctcaaa
     9421 ttcaggcgtt gaaaacgctc gaccagcgtc cagcggagaa attcgcccag cagatgtatg
     9481 agacgcgcta tgctgatgac cgcacgaaat tactgcaaga aaatcagatt gcacagttta
     9541 ctgccgcaga tgcgctggct gccgatcgcc agttgttttc atctccagcg gatatcacgt
     9601 ttgtcattgt cggtaatgtc gcagaagaca aactcatggc gttaattacg cgttacttag
     9661 gctcaatcaa acactctgat tcgccattag ccgcaggtaa accattaact cgcgcgacgg
     9721 acaacgcatc ggttactgta aaggaacaaa atgaacccgt ggcacaggtt tcacagtgga
     9781 agcgttatga ttcccggaca cctgttaatc tggcgacgcg tatggcgctc gatgctttta
     9841 acgtcgcact ggcaaaagat ctacgcgtta atattcgtga acaggcatct ggggcataca
     9901 gcgtttcttc tcgcctctcg gttgatcctc aggccaaaga tatcagtcat ttgctggctt
     9961 ttacttgtca accagaacga catgatgaac tgttaacgtt agcgaatgaa gtgatggtta
    10021 agcgtctggc taaagggatc agtgagcaag aactgaacga ataccagcaa aacgttcagc
    10081 gcagcctcga tatccaacag cgtagcgttc aacaattagc gaacactatt gtaaatagtc
    10141 ttattcaata tgacgatcct gcagcatgga ctgagcagga gcaattgttg aaacaaatga
    10201 cggtagagaa tgttaacact gccgttaaac aatatctttc tcatccggta aatact
//
LOCUS       DAZGBC010000167        10154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGBC010000167 DAZGBC010000000
VERSION     DAZGBC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.168"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..910
                     /locus_tag="ACHK1J_004155"
                     /pseudo
     CDS             23..910
                     /locus_tag="ACHK1J_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            923..1837
                     /locus_tag="ACHK1J_004156"
     CDS             923..1837
                     /locus_tag="ACHK1J_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7913358.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLTCEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKNRMAKLFNQSDTSVRNKYRPAFDKWNELV
                     GKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2530..2748
                     /gene="yiiE"
                     /locus_tag="ACHK1J_004157"
     CDS             2530..2748
                     /gene="yiiE"
                     /locus_tag="ACHK1J_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO7913359.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2965..3207
                     /gene="yiiF"
                     /locus_tag="ACHK1J_004158"
     CDS             2965..3207
                     /gene="yiiF"
                     /locus_tag="ACHK1J_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO7913360.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGREEDGVEYQRKLREEW"
     gene            complement(3537..4466)
                     /gene="fdhE"
                     /locus_tag="ACHK1J_004159"
     CDS             complement(3537..4466)
                     /gene="fdhE"
                     /locus_tag="ACHK1J_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO7913361.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4463..5098)
                     /gene="fdoI"
                     /locus_tag="ACHK1J_004160"
     CDS             complement(4463..5098)
                     /gene="fdoI"
                     /locus_tag="ACHK1J_004160"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7913362.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5095..5997)
                     /gene="fdoH"
                     /locus_tag="ACHK1J_004161"
     CDS             complement(5095..5997)
                     /gene="fdoH"
                     /locus_tag="ACHK1J_004161"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO7913363.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(6010..9060)
                     /gene="fdnG"
                     /locus_tag="ACHK1J_004162"
     CDS             complement(6010..9060)
                     /gene="fdnG"
                     /locus_tag="ACHK1J_004162"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8473..8475),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7913364.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9254..10087
                     /gene="fdhD"
                     /locus_tag="ACHK1J_004163"
     CDS             9254..10087
                     /gene="fdhD"
                     /locus_tag="ACHK1J_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO7913365.1"
                     /translation="MKKTQQKEIENVTNVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2576 a         2435 c         2578 g         2565 t
ORIGIN      
        1 taactgtgga aaggaggctg taatggttgc caatattaat ctcattcaac aagaaagtta
       61 ttctgttgtt aatttagaaa aacaactaat gagtaatgaa tcaccgaagc aaacgctatt
      121 aaatgatttt tcacatcgcc ttaaaatgga aggtgaggaa caactgttcc attgtgttaa
      181 cgaactgaaa tatggtttgt cgagtaataa tgaatatgac ctgtttaaaa atagagaaac
      241 ctcactgtgg aaacgcaaag taccagaaag cgcagccctg gcaatgacgg gaacagtata
      301 taaattattt ggtagtcgct tactcaacgc ccgcaatcag ttagataaaa atctcctcga
      361 aatggctatg caaatggttt atggcaaaga tatggtgaca aagtccgggg atatattgat
      421 tgatatacaa ttaaataagg atggcagcct ccagagtaag cactacacat ttgatgtagg
      481 tgaagttata aacagttata atattgacct ctttgtgaat gcttcgagta ttaatcaaac
      541 ttacttacat gataaaaatc ctggtgacgt tattagcctg tatggcactt tagataacat
      601 tccccttgta gttaaggaat gcacgggtaa aaatcatcaa attaacgtta aagccaaatt
      661 gccacaagaa cgaagtgaaa aagttgagga tatgtgtgaa agaatgagag gtggcatctc
      721 tatgtttaat cacaccacaa aaacagctgg taatatagag cataatttgc agctaagttt
      781 tctcgtggat tcacgaccgt cgatcaccaa taaatactca gccaaagatg tagcgccaga
      841 catacttgta ttgcccgtca acgtatatca tgcggatttt aaaattaaaa tcaatgacgc
      901 agtagagaag gcttcgtcaa ttgtgttatg tgctgtgcaa aaaaatctcc atgcagcaag
      961 aaaggttctc agcgggataa taggaaatgt gccaaaaaat aataaaccaa aaacaatcat
     1021 ttttgattta cgttttctta gtgcaattaa gcagaaagtt tttttgggca gtgaaaaacc
     1081 gttgtttgta aagcatacgg caatgatgag ggaggcatgt aaggaattac cgcagtcagt
     1141 ggaatatgtt cctttaacct gtgaagcgca tagccatcgc agcgtcgccc ttgccaaaac
     1201 cgtggctgcg cccatcatgg cgacaggtgt gggcgcggct ttaatgtatg caacatcatc
     1261 aggaccgtat tatgcgcaaa gtctttctgg ttcgccggat atcgagatga taaaaaatcg
     1321 catggctaaa ttgtttaacc agtcggatac ttctgtgcgc aacaaatatc gcccggcttt
     1381 tgataaatgg aatgaactgg taggtaaatt aaatcacgaa agaaatacag ttccgtttgc
     1441 gtgtttgacc accattttag cgacagcgat aaatgaaact cctgaggggg atacgaatgt
     1501 tgccgttgtg atggggtgta aatctgcgaa agatcgcacg atatccattg tccttggcaa
     1561 ctcgatgttg caaacactct ttgaaaaacg cctcgccgat gatggagaaa tagaaaacct
     1621 ttttgatcaa cagggatatt ttagttgcga ttcattaaca gccgaagaag taatgatgtt
     1681 aaaagacctt tttgatattc gagttcttca tctctctaac aaatttaatg ttgggttgca
     1741 aggtaatatc aacacggatg ttttacaaga ttcgtttttt aaaaatgtcg atttcattca
     1801 tgaatctaac tcgtataccg tagggatggg agtctgaaga gtaaaactca ttaggattat
     1861 cagaattcgg ccaatgcatt gcaaaaatga aatacaaaga gagatgtgtg caggcgctta
     1921 gccgcgagag gcatggcgaa gccatacaga aagcaccagc attcagaact cctgtgaaag
     1981 cggcatagtg ccgctgctca ggagttttgt tatgacccgt atcgtgctga gcgttgccac
     2041 cctatgatgc gattaaaaaa actcaccgtc gagccgatga ttgacgtcaa tgctatcgga
     2101 tgatgccggg ggtgttccgg aaggaattcc ttgtttcccg aactgttacc aacaaaatta
     2161 taacgatgag caagtaaaga ccggttggtg gcagtactga ccggtctgtg atggaagatt
     2221 aatttcacct taaatcctgg tgctgttagg atgctgacat ttttcgggta caccattcag
     2281 attatgcaaa taaagatcca atgaaaaaat aaatatccct gaatataata tattcatagc
     2341 acccataact catcctgggc gataactaaa ccatttttga gtaacaaatg aaacaactgc
     2401 aacgctctta ttatttctgg aaaacgtctc gcaaaaacaa aacaacatgc aaaaatccgc
     2461 tttcgttaat taattttccg gctaattttc gcacatacat taatgagtaa tatatgtaag
     2521 agggtggcaa tggcgatgaa tacggttttt cttcatttat cagaagaggc aattaaacgg
     2581 ctgaacaagc ttcggggctg gcgtaaggtt tcacgatctg caattttacg cgaagcggta
     2641 gagcaatatc tggaacgtca gcaattcccg gtgcgtaaag caaagggagg caggcaaaag
     2701 ggggaggtag ttggtgttga tgatcagtgt aaggagcata aggaatgaca aaaatttttc
     2761 attcttgaat ataaaaaaca gatgccctta ttctggtatt aatacaaggc tattttactt
     2821 gaacttataa taactgcaac tgttacatca tatctggaaa acgcctcgca aaaataaaaa
     2881 atgatgcgta aatgagcttt ctttaaagat tcgacaaggg tacttttctc tccatagata
     2941 tcaaatgata ttggtcaaat atttatgaac tctctggcag gtattgatat gggcagaatt
     3001 atactcgatt tatcgaatga ggtgattaag caacttgatg atcttgaggt gcagcgtaat
     3061 cttcctcgta cggagctatt acgggaagcg gtagatcaat acttggtaaa tcaatcgcaa
     3121 acagtaagaa ccagtgcttt tggcatatgg caagggcgtg aggaagatgg tgtcgaatac
     3181 cagcgtaagc tgcgcgagga atggtaatgg gaaaaaatga tatcaatcag attgctgaca
     3241 acgtgtgcgt tgttcatgcc ggatgcggcg tgaacgcctt acccggccta caaagtcgta
     3301 taaattcaat gtattgcagt ttattggtag gcctaataag cgcggcgcat caggcaatct
     3361 tacgcttatc atcagtctca tacccataac cttattgatg tgggggtggg taaattgagg
     3421 caaaacaggc actggcggca tagcttgagg tggtaacgcg gaatacgaga gatggtgtcg
     3481 tcacacccta tgagctgtaa tccgggcgac cagcgccacc cggaaaacgt cagagattac
     3541 tccccttcac ccggaaacag gaacgggttg atggaactgc gggcatagcc ttcttgctcc
     3601 attcgtgcgt ccagtaccag agaggcgagg tcatctgcca cggcttcaac tttcggttct
     3661 ttttcctgat agagaatttt caggtaagtg ccgcagtcat cgcagctttc ggctttaatc
     3721 gcggcctgtt cgtcatccag cgaccagtaa tgcagtttgc cgctctgctc acagttgctg
     3781 cattttacgc gcactacgtg ccattcagtt tcacacaggt tgcagtgcag gtaacgcaga
     3841 ccctgagtgg tgccaatttg caccatgctg gacaccggca tagagccaca taccgggcaa
     3901 tattgacgtt gttcgccgta ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc
     3961 gcccagtaga gcgacagtgc agcccagata aacggcgctt tatcgctact gacggatgag
     4021 aaatcagagg caaacagtgc gctggccata tcttccagct cctgagtcga tgccttctcc
     4081 agattctcaa tcactgccag cgccgggccg ctcatttcag gtttcagctc agcaattagc
     4141 gccatcagca gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc
     4201 ttgccttgtg cgctggcttc tttaatgcgt gcagtcagat ccatctccag cggatggtcg
     4261 tacagcacca cttcctgggc gtgggcgata agcgcagcaa agcgcaggta atcacccagc
     4321 ggattatttt ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa attcttgagc
     4381 cgagggaaca ataacggcgg aatcatatcc gccgtacgtt tctcgctcga acccagctca
     4441 tcttgcggga ttatgcgaat actcattcag ctttcttttc cgttgtcttg cggacctcac
     4501 ggtaccagcg cgggtgatgt ttcttcgccc aggcgctggt aacccatcct tccaccatgg
     4561 cggtaatcgt gcctttcacc caaagggcgg cgtagatatg caccatgata accacaatta
     4621 acgccactgc ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg attgagaaag
     4681 caggcgcaaa ataaggacgc cagataatca cgccactcac cagcagcaga accaggaaaa
     4741 taatcgccgc ccagaaaacg catttctgac cgaagttata acgcccggtg tcacctactt
     4801 cctcgttgac gacgatctta cgaatattct tcgcccaaaa gatatcatcc cgattgatta
     4861 ggttgtgatg ccagtaacgg aaaaacatga tgatgaacga ggcaaacata accacgccga
     4921 caaacgggtg cagaattcgc gccagctgcg gtgtgcccat gatttgcatc aaccagttga
     4981 aggacgggaa caaaaagccc agcccgctca ccgccgccag gatgaagcag aaggcggtga
     5041 tccagtggtt gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt
     5101 gcgctcctcg tctttctctt cgtgcagatt attctcttcc tcatccgcac ggttcggacc
     5161 gacacccacg tagtggaaga tactggctgc gaaggtagcc gcaaagccaa cagctgcgag
     5221 cggtttccag atgcctttcc agaatttcac ggtttcgctg atttccgggt tctccggcaa
     5281 gccatgatac agatttggct tgtcagcatg gtgcagcacg tacataacgt gtgtaccacc
     5341 gacgcctgcc ggatcgtaca ggcccgcatt gtcgtaaccg cgggttttca gctccgccac
     5401 gcgctcgctc gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg
     5461 gcaggtcttc acgcaggctg gttcttgccc aaccaccacg cggtcaacgc acaacgtaca
     5521 tttgtagacg cggttgtctt ccgggttgag gcgcggaatg tcgaacggac agcccgcaat
     5581 gcaataaccg cagccaatgc actgctcgga ctggaagtcg acgataccgt tggcatactg
     5641 aataattgca ccttccgccg ggcacgcttt cagacagcct ggatcggaac agtgcataca
     5701 gccgtctttg cggatcagcc attccagttt gtcgttctgc tccacttccg agaagcgcat
     5761 caccgtccac gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc
     5821 gacggtatcg cgaatgtcgt tccactctga acacgccacc tgacaggctt tacagccgat
     5881 acaggtggtg acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc
     5941 gggggtcaga ccgttagtcg cggaacgacg aatgatatct tgcgattgat aagccatatg
     6001 tcgtctccgt tacacctttt ccacattcac aaggaaggac ttaaactccg gcgtctgcgt
     6061 gtttgcatca ccaacgaatg gcgttaacgt attggcaata aagccttttt tagctacgcc
     6121 ttcataaccc cagtgaatcg gaataccgat ggtatcgata tctttgccgt tcgctttcag
     6181 cgtgcgaata cgtttggtca ccaccgcttt ggctttgata tagccacggt tggaggagac
     6241 tttcacggta tcgccctggg caatgccaag tttattcgcc agcgactccc cgatttccac
     6301 aaactgttct ggttgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt agtggaaatg
     6361 ctcggtcaga cgataggtgg ttccgacata cgggaactta tcggctttac ccaatgcttc
     6421 ggcgtcgtct ttaaagattc gcgcagccgg gttcgagata acgtttggat gcagcgggtt
     6481 agttcccagc ggcgtttcaa acggctcgta gtgttccggg aacggacctt ccgccatctt
     6541 atcgagggca aacagacgcc ccatgccttc ctgctgcatg ataaacggcc cgacgccgct
     6601 acccggaggc gctgcgctgt agtccggaat atcccagccg gtccacttag tgccgtccca
     6661 tttcagcaac tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt
     6721 atacagaatg cggcggttaa gcggccatgc ccatgcccag cccagcgtgt taccgaggcc
     6781 agacggatcg gcgttatcac gacgcgccat ctggttgcct tccggcgtcc agctaccggc
     6841 gaaaatccag cagccacagg aggttgtacc gtcatcgcgc aattgggcga acgagctaag
     6901 ttgttggcct ttcttgacga taaccgcacc ggttgccgga tcggtaatat cggccagcgc
     6961 cttaccgttg ctctccatcg ctacttcttc cgacttcggc tcatgcggaa tggcgtagtt
     7021 ccaggtcatg ttcagcacct ggtccgggtt cgcgccaccc tgttcggcat acatcttgcg
     7081 caagcgcagg aagataccgg agaggatctc gccgtcagtc agcgcaatcc ccggggcgtc
     7141 cgcacctttc cagtgccact gcaaccagcg acctgagtta acgattgaac cgttctcttc
     7201 tgcgaagcag gtcgatggca gacggaacac ttcggtctgg atcttcgacg agtcaacttc
     7261 gttcagctca ccgtggttct gccagaagtt agaggtttca gtgttcagcg ggtcgatggt
     7321 gacgaggaac ttcagtttcg acagacaacc gatcactttg tttttgttcg ggaatgaggc
     7381 aacagggtta aagccctggc agatatagcc attgaccttg ccctctttca tcatctcgaa
     7441 gtattgcagg acgtcgtagc ctttatccca cttcggcaac cagtcaaagc cccagctatt
     7501 ttccgccgtc gctttatcac caaagaaggc cttcatcata gagacgaaga atttcgggta
     7561 gttgccccag tagtttacct ggccttccag cagcggtttt ggcgtgttgg cggtaaggta
     7621 ggtttgcaga tcggtctgct tctcgcttgg cagcgtcatg taacccggca ggctctgcga
     7681 cagcagcccc aggtccgtca gcccctgaat attggagtgg ccgcgcaggg cgttaacgcc
     7741 gccgccagcc atccccatgt taccgagcag caactggatc atcgccatcg tacgaatgtt
     7801 ttgcgcacca acggagtgtt gcgtccagcc gagggcatac aggaacgagg cagttttatc
     7861 gtgagcactg gtttctgcga tgtattcgca gactttcagg aacgcgtctt ttggcgtacc
     7921 acagatgttt tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca
     7981 cacgcagcgc ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc
     8041 ataagtccag gaggatttat cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc
     8101 atcttcaaag ccgtaatcct cacgcacgat caggctggcg ttggtatagg cttcagtgta
     8161 ttcgcggttg aatttttcat tgttcagcag gtacagcaat acgcctgaca ggaaagcaat
     8221 gtcagtaccg gaacgaatag gggcatagta gtcagctacc gccgccgtac gcgtaaagcg
     8281 aggatcgatc acaatcagct tagcgccgtt gtgaattttg gcttccatcg cccagcggaa
     8341 cccgaccggg tgagcttcag cggcgttacc gcccatcacc acgacgaggt tggcgttctt
     8401 gatgtcgacc cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac
     8461 cgttggtccg tgtcagacac gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga
     8521 gaatttttgc gttaaatagc cggtttcgtt actcgacgcg gaagcacaca gcatcccggt
     8581 ggagagccag cggttaacag tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg
     8641 gtcttctttc atcagtttgg cgatgcgatc aaacgcctct tcccaactga tttgctgcca
     8701 tttatcagaa cctggcgcac ggtattccgg aaacttcaga cggctttcgg agtggatgaa
     8761 atccaccagg ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc
     8821 gccttcgata tggaagatag atgctttggc gttttttgct ccgtcaccga ggctgtacat
     8881 caacagccca cagcctacgg aacaataggt gcaggtatta cgggtttcgc gggtgcgcag
     8941 cagtttatac tgccgggttt ccgcgagtgc tacgctgggt gcaaaaccca gtgccgctgc
     9001 cgtggtgcct gccataccgc cagcgcagat cttaaagaac tgccttctgc tgacctgcat
     9061 ggattgctcc ttgtttcgac attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag
     9121 ggagtgggag ttatttttct ttgcggaagt ggccgcaaag gtccagaatt ggctcaattt
     9181 ccctccatcc aggaagggtt tgtaacagaa taccataatg ttggtgtgtg tgttcttatc
     9241 cggttaatag aaagtgaaaa aaacacagca aaaagaaatc gaaaatgtga caaatgtcac
     9301 aggtgttcgt caaattgagt tatggcggcg tgatgattta caacacccac ggttagatga
     9361 ggtggcggaa gaagttcccg ttgcgctggt ctacaacggc atttcgcatg tggtgatgat
     9421 ggcgtcgccc aaagaccttg agtactttgc gctcggtttt tcgctttccg aggggataat
     9481 agaaagtccg cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt
     9541 acaaattgag ctttccagcc gccgctttat ggggttgaag gagcgccgcc gggcgctggc
     9601 gggacgtacg ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt
     9661 gcagccgcta ccgttcaccc agacgtttga tctcaacaaa ctggatgatg cattacgtca
     9721 tctcaacgat tttcagccag tgggacaact gactggttgt actcacgccg ctgcctggat
     9781 gttgccatct ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg tagcgctgga
     9841 caaactgtta ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg cggtactggt
     9901 ttccagtcgt gccagttatg aaatggtgca aaagtcggcg atgtgcggcg tagagatttt
     9961 gtttgcggtg tctgccgcga ccacgcttgc tgtagaagtg gctgagcgct gtaatttgac
    10021 actggtaggt ttttgtaaac cgggtcgggc aacggtttat acccatccgc agcgtttgag
    10081 caattaatta aaataagtcc tgcgatatat attaaattgc aggacttatt catttcgtga
    10141 attttattgt ttta
//
LOCUS       DAZGBC010000168        10149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGBC010000168 DAZGBC010000000
VERSION     DAZGBC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.169"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            423..1400
                     /gene="glaH"
                     /locus_tag="ACHK1J_004164"
     CDS             423..1400
                     /gene="glaH"
                     /locus_tag="ACHK1J_004164"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO7913366.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLAQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1419..2687
                     /gene="lhgO"
                     /locus_tag="ACHK1J_004165"
     CDS             1419..2687
                     /gene="lhgO"
                     /locus_tag="ACHK1J_004165"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO7913367.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARNVDALHAAFNQ"
     gene            2710..4158
                     /gene="gabD"
                     /locus_tag="ACHK1J_004166"
     CDS             2710..4158
                     /gene="gabD"
                     /locus_tag="ACHK1J_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7913368.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGD
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4172..5452
                     /gene="gabT"
                     /locus_tag="ACHK1J_004167"
     CDS             4172..5452
                     /gene="gabT"
                     /locus_tag="ACHK1J_004167"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO7913369.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPNAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFAEAKQ"
     gene            5870..7270
                     /gene="gabP"
                     /locus_tag="ACHK1J_004168"
     CDS             5870..7270
                     /gene="gabP"
                     /locus_tag="ACHK1J_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO7913370.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7291..7953
                     /gene="csiR"
                     /locus_tag="ACHK1J_004169"
     CDS             7291..7953
                     /gene="csiR"
                     /locus_tag="ACHK1J_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO7913371.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMNLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEARDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRQTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMCQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7954..8403)
                     /gene="kbp"
                     /locus_tag="ACHK1J_004170"
     CDS             complement(7954..8403)
                     /gene="kbp"
                     /locus_tag="ACHK1J_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO7913372.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8487..8645)
                     /locus_tag="ACHK1J_004171"
     CDS             complement(8487..8645)
                     /locus_tag="ACHK1J_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO7913373.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8828..9127
                     /locus_tag="ACHK1J_004172"
     CDS             8828..9127
                     /locus_tag="ACHK1J_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7913374.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9137..9655
                     /gene="ygaP"
                     /locus_tag="ACHK1J_004173"
     CDS             9137..9655
                     /gene="ygaP"
                     /locus_tag="ACHK1J_004173"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO7913375.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(9708..10112)
                     /gene="stpA"
                     /locus_tag="ACHK1J_004174"
     CDS             complement(9708..10112)
                     /gene="stpA"
                     /locus_tag="ACHK1J_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO7913376.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2303 a         2639 c         2796 g         2411 t
ORIGIN      
        1 aggcggaact ctaaatatca atattcctgg gcgtagcgct cttcttttag ggaaaacagg
       61 agaaccgccg aactatctct atttgtaatt taatgttata tctgccccgc taaaacgggg
      121 cagataatat gtttagttta ctaacggtca ttttgcattg aagccattta ctgtttttta
      181 tcgaccagat aatctgttct ctgatggtaa ctccccctaa cctgttgctt tagttattca
      241 tttcctgtct cactttgcct taatacccta cgttaaatgt tactaatttg ttgtttttga
      301 tcacaataag aaaacaatat gtcgcttttg tgcgcatttt tcagaaatgt agatattttt
      361 agattatggc tacgaaatga gcatcacgca ctctccctga aacctcaaga ggatcgcttc
      421 tgatgaatgc actgaccgcc gtacaaaaca acgctgtcga ttcaggccag gactatagcg
      481 gattcaccct caccccatcg gcgcaatccc cgcgtctgct ggaactcacc tttaccgaac
      541 agacgaccaa acagttcctc gcgcaggttg ccgagtggcc cgtgcaggcg ctggagtaca
      601 aatcgtttct gcgttttcgg gtaggcaaaa ttcttgatga tctgtgtgcg aatcagctgc
      661 aaccgttgct gttgaagacc ctgctaaacc gcgctgaagg tgcgttgttg atcaatgcgg
      721 tgggggtcga tgatgtgaag caagcggatg agatggtgaa gctggcaacg gcggtggcgc
      781 atctgattgg tcgctccaat ttcgacgcga tgagcggtca gtattacgcg cgttttgtag
      841 tgaaaaatgt cgataactca gacagctatc tgcgtcagcc gcaccgcgtg atggagctgc
      901 acaacgacgg cacctacgtg gaagagatca ccgactatgt gctgatgatg aaaatcgacg
      961 agcaaaacat gcagggcgga aattcgttgc tgctgcatct cgatgactgg gaacatctcg
     1021 accactattt ccgccacccg ctggcgcgcc gcccaatgcg ctttgccgcg ccgccgagta
     1081 aaaacgtcag caaagatgtt ttccatccgg tgttcgacgt tgatcaacag ggtcgcccgg
     1141 tgatgcgtta tatcgaccaa ttcgtccagc caaaagattt tgaagaaggc gtgtggttga
     1201 gcgagctttc cgatgccatt gaaaccagca aaggcattct ttctgtgccc gttcccgttg
     1261 gcaaattcct gttgattaac aacctgttct ggctgcacgg tcgcgaccgc tttactccgc
     1321 acccggatct gcgccgtgaa ctgatgcgtc agcgtggcta tttcgcttac gccactcacc
     1381 actaccagac gcatcagtag cgcaaaggaa ttgagcggat gtatgatttt gtgattattg
     1441 gcggtggcat catcggcatg tcgaccgcca tgcaactgat tgatgtctac ccggacgccc
     1501 gcattgcgtt gctggaaaaa gagtccggcc cggcctgtca ccagacgggg cacaacagcg
     1561 gcgtgatcca tgccggggtc tattacacgc ccggcagcct gaaggcacag ttttgcctgg
     1621 cggggaaccg cgccaccaaa gccttttgcg atcaaaacgg cattcgctac gacaactgcg
     1681 gcaagatgct ggtcgccacg tccgatctcg aaatggaacg gatgcgcgcg ttgtgggaac
     1741 ggacggcggc gaacggcatc gagcgcgagt ggttaaacgc cgatgaactg cgcgagcgcg
     1801 aaccgaatat caccgggctt ggcggtattt ttgtgccgtc cagcggcatt gtcagctacc
     1861 gcgaagtcac ggcggcgatg gcaaaaatct tccaggccag aggcggcgag attatctata
     1921 acgccgaagt cagcggcctt agtgagcata aaaacggcgt ggtgatacgt acccgtcagg
     1981 gtggcgaata cgaagcgtca acgctgatta gctgttccgg gctgatggct gaccggctgg
     2041 tgaaaatgct cggcctcgaa ccgggcttta ttatctgccc gttccgtggc gagtatttcc
     2101 gcctggcgcc ggagcataac cagattgtta accacctgat ttaccccatc cccgacccgg
     2161 caatgccgtt tttaggcgtt catctcaccc gcatgatcga cggtagcgtc accgttgggc
     2221 caaacgcagt gctggcgttc aaacgcgaag gctatcgcaa gcgcgacttc tcgtttagcg
     2281 acacgctgga gattttaggc tcgtcgggga ttcgccgggt gctgcaaaac catctacgct
     2341 caggactggg cgagatgaaa aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa
     2401 agtattgtcc ccggctttcg ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc
     2461 aggcggtatc gccggacggc aagctgattg acgattttct gtttgtcacc accccgcgca
     2521 cgatccacac ctgcaatgcg ccctccccgg cagcgacatc agcaattcct attggtgcgc
     2581 atattgtcag caaggtacaa acgctgttgg caagccagag taaccccgga cgcacgctgc
     2641 gagcggcacg taatgtggat gccttacacg ccgcatttaa tcaataacct ttgaaaacag
     2701 gatgtagcga tgaaactgaa cgacagcaac ttattccgcc aacaggcgtt gattaacggg
     2761 gaatggctgg acgccaacaa tggtgaagtc atcgacgtca ccaatccggc gaacggcgac
     2821 aagctgggta gcgtgccgaa aatgggcgca gatgaaaccc gcgccgctat cgacgccgcc
     2881 aaccgcgccc tgcccgcatg gcgcgcgctc accgccaaag aacgcgccaa cattctgcgc
     2941 aactggttca atttgatgat ggagcatcag gacgatttag cgcgcctgat gaccctcgaa
     3001 cagggtaaac cgctggctga agccaaaggc gaaatcagct acgccgcctc ttttattgag
     3061 tggtttgccg aagaaggcaa acgcatttat ggcgacacca ttcccggtca tcaggccgat
     3121 aagcgcctga ttgtgatcaa gcagccgatt ggcgttaccg cggctatcac gccgtggaac
     3181 ttcccggcgg cgatgattac ccgtaaagcc ggcccggcgc tggcggcagg ctgcaccatg
     3241 gtactgaagc ccgccagtca gacgccgttc tctgcgctgg cgctggcgga gctggcgatc
     3301 cgcgcgggca ttccggctgg cgtgtttaac gtggtcaccg gttcggcggg cgcggtgggt
     3361 gacgaactga ccagcaaccc gctggtacgc aaactgtcgt ttaccggttc gaccgaaatt
     3421 ggccgccagt taatggaaca gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc
     3481 ggcaacgcgc cgtttatcgt ctttgacgat gccgatctcg ataaagccgt ggaaggcgca
     3541 ctggcctcga aattccgcaa cgccgggcaa acctgcgtct gcgccaaccg tttatacgtg
     3601 caggacggcg tgtatgaccg ctttgccgaa aaattgcagc aggcggtgag caaactgcat
     3661 atcggcgacg ggctggataa aggcgtcacc atcgggccgc tgatcgatga aaaagcggta
     3721 gcaaaagtgg aagagcatat tgccgatgcg ctggaaaaag gcgcgcgcgt agtttgcggt
     3781 ggtaaagcac acgaacgcgg cggtaacttc ttccagccga ccattctggt ggacgttcca
     3841 gccaacgcca aagtgtcgaa agaagagacg ttcggccccc tcgccccgct gttccgtttt
     3901 aaagatgaag ccgatgtgat cgcgcaggcc aatgacaccg agtttggcct tgccgcctat
     3961 ttctacgccc gtgatttaag tcgcgtcttc cgcgtgggcg aggcgctgga gtacggcatc
     4021 gtcggcatca ataccgggat tatttccaat gaagtggccc cgttcggcgg cattaaagcc
     4081 tcgggtctgg gtcgtgaagg ttcgaagtat ggcatcgaag attacttaga aatcaaatat
     4141 atgtgcatcg gtctttaact ggagaacgcg aatgagcagc aataaagagt taatgcagcg
     4201 ccgcagtcag gcagttcctc gtggcgttgg gcaaattcac cccattttcg ctgatcgcgc
     4261 ggaaaactgc cgggtgtggg acgttgaagg ccgtgagtat cttgatttcg cgggcggcat
     4321 tgcggtgctc aataccgggc acctgcatcc gaaagtggtt gcggcggtgg aagcgcagtt
     4381 gaaaaaattg tcgcacacct gcttccaggt gctggcttac gagccgtatc tggagctgtg
     4441 cgagattatg aatcagaagg tgccgggcga ttttgccaag aaaacgctgc tggtcaccac
     4501 gggttccgaa gcggtggaaa acgcggtgaa aatcgcccgc gctgccacca aacgtagcgg
     4561 caccatcgct tttagcggcg cgtatcacgg gcgcacgcac tacacgctgg cgctgaccgg
     4621 caaggtgaat ccgtactctg cgggcatggg cctgatgcca gggcacgttt atcgcgcgct
     4681 ttatccttgc ccgctgcacg gcatcagtga agatgacgct atcgccagca tccaccggat
     4741 cttcaaaaat gacgccgcgc cggaagatat cgccgccatc gtgattgagc cggttcaggg
     4801 cgaaggcggt ttctacgccg cgacgcctgc gtttatgcag cgtttacgcg cgctgtgtga
     4861 cgagcacggg atcatgctga ttgccgatga agtgcagagc ggcgcggggc gtaccggcac
     4921 gctgtttgcg atggagcaaa tgggcgtggc accggatctc accacctttg cgaaatcgat
     4981 cgcaggcggt ttcccgctgg cgggcgtcac cgggcgcgcc gaagtgatgg atgccgtcgc
     5041 tccaggcggg ctgggcggca cctatgcggg taacccgatt gcctgcgtgg ctgcgctgga
     5101 agtgttgaag gtgtttgagc aggaaaatct gctgcaaaaa gccaacgatc tggggcagaa
     5161 gttgaaagac ggattgctgg cgatcgccga aaaacacacc gagatcggcg acgtacgcgg
     5221 gctgggggcg atgatcgcca tcgagctgtt tgaagacggc gatcacagta aaccgaacgc
     5281 caaactcacc gccgagatcg tggcgcgcgc ccgcgataag ggcctgattc ttctctcctg
     5341 cggcccgtat tacaacgtgc tgcgcatcct tgtaccgctc acaattgaag atgctcagat
     5401 tcgtcagggt ctggagatca tcggccagtg ttttgctgag gcgaagcagt agcaccgccc
     5461 ccatgccgga ggcgacgctg cgcgtcttgt ccggcctacg agagtgtagg tcggataagg
     5521 cgtttacgcc gcatccgaca atcggcgcac catgccggag gcgacgctta cgcgtcttgt
     5581 ccggcctacg gaaatgtagg tcggataagg cgtttgcgcc gcatccgaca atcggcgcac
     5641 ccatgccgga ggcgacgctt tgcgtcttgt ccggcctacg gggattctcg tcggataagg
     5701 cgtttacgcc gcatccgaca atctgtacgt gaacaggaag aaatctatgt tggccgggta
     5761 aggcggagcc gctctccggc aaaaagaatc aataacaatt aaacgcgtga cccgacgcgg
     5821 gaaatgtcgg ggcgctctcc caagtgacac actttcgaga ggattcagga tggggcaatc
     5881 atcgcaacca catgagttag gcggcgggct gaagtcacgc cacgtcacca tgttgtctat
     5941 tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc gtcgccatcg ccgaagcggg
     6001 cccggcggta ttactggcct atctgttcgc cggattactg gtggttatga ttatgcggat
     6061 gttggcggaa atggcggttg ccacgcccga taccggttcg ttttccacct atgccgataa
     6121 agccattggc cgctgggcgg gctataccat cggctggctg tactggtggt tttgggtact
     6181 ggttatccca ctggaagcca acatcgccgc catgatcctg cactcgtggg ttccaggcat
     6241 tcccatctgg ttattttccc tcgtcattac cctcgcctta actggcagta atttattaag
     6301 cgttaaaaac tacggcgaat ttgagttctg gctggcgctg tgcaaagtca tcgctatcct
     6361 ggcctttatt ttccttggtg cagtcgcaat tagcggtttt tacccgtatg ccgacgtgag
     6421 cgggatctca agattgtggg atagcggcgg ctttatgccc aacggtttcg gtgcggtatt
     6481 aagtgcgatg ttgatcacca tgttctcgtt tatgggcgca gaaattgtca ccattgccgc
     6541 cgcggaatcc gacacgccgg aaaaacatat tgtccgcgcc actaactcgg ttatctggcg
     6601 tatttctatc ttctatttgt gctctatttt tgtcgtcgtg gcattaatac cgtggaatat
     6661 gccggggctg aaagccgttg gttcttatcg ctcggttctg gaattgctca atattcccca
     6721 tgcgaaatta atcatggact gcgtgatatt actttccgta accagttgcc tgaactcggc
     6781 gctgtatacc gcttccagga tgctctactc cttaagccgt cgcggtgatg cgcccgcggt
     6841 aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg gtgttactct ccaccggcgc
     6901 ggcattttta acggtggtgg tgaactatta cgcacctgcg aaagtgttta aattcctgat
     6961 cgacagctcc ggcgctatcg ccctgctggt ttatttagtc atcgccgttt cacagttgcg
     7021 gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc ctgcgcatgt ggctttaccc
     7081 gtggctcacc tggctggtca tcggctttat tacctttgtg ttggtggtga tgctattccg
     7141 cccggcgcaa cagttagaag tgatctccac tggcttatta gcgataggga ttatctgtac
     7201 cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa
     7261 tacgcgctga taatgatcag gagtcacacc atgaccatta cgtctctgga tggctatcgc
     7321 tggctgaaga acgatattat tcgcggtaat tttcaaccgg atgaaaaatt acgaatgaat
     7381 ttgctgacat cgcgttatgc acttggcgtt gggccgttac gggaagctct ttcgcaactg
     7441 gtggcggaac ggctggtcac ggtggtgaat caaaaagggt atcgggtggc gtctatgtca
     7501 gagcaggagc tgctcgatat tttcgacgcc cgcgccaata tggaagcgat gttagtgagt
     7561 ctggcgattg cccgcggtgg cgatgagtgg gaggcagacg ttctcgcaaa agcgcatctg
     7621 ttgagtaagc ttgaggcccg tgacgccagc gagaaaatgc ttgatgagtg ggatctgcgt
     7681 catcaggcgt ttcatacggc aattgtggcg ggctgtggtt ctcactattt gctgcaaatg
     7741 cgtgaacggt tgtttgatct ggcggcgcgc tatcgattta tctggctgcg acaaacagtg
     7801 ctttcggtgg aaatgctgga ggataaacac gatcagcacc agaccctgac tgcggcggta
     7861 ctggcgcgag ataccgcgcg cgccagtgag ttaatgtgcc agcatttact gacgccaatt
     7921 cccattatcc agcaggcgat ggctggcaat taactactct tcaggaatac gcaacacttg
     7981 ccccgggtaa attttatccg ggctttttag catcggttta ttcgcttcga agattttatt
     8041 gtacagattg gcgttaccgt agacctgttt ggaaatggca ctcagagtgt cgccagactt
     8101 aacggtataa aactggctgg cagtggctgg tgtcgccgtt ttcacctgat catcgacact
     8161 ggcaataccg gaaatattcc ccaccgcaac aaggattttc tccttcgcct cctgactcag
     8221 gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga atgttcactt tatcggcatc
     8281 aggtataccg gttttgctca gatgctcctg caccttcttc gcctgatcgt ctttatcgtg
     8341 ctgacctgta accgcgtccc agagtttttc tccggcatct ttcacaaaat tgaacagccc
     8401 catagctacc tcattcgtta acggaaacac tcagaaagtg tagcagagga acgcggcgcg
     8461 ttaacgccgc ggggatgagc acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc
     8521 aaaccgggga tatacccaag cagtgtcaac agaatattaa taatgaacgc ccaaccgaac
     8581 cctttaccga gcagcacgcc gagcggcggc agaatgatgg tgatgacgat tctccagaaa
     8641 cccatatgta ctccctataa gaaaattact cattgtttaa aaagagattt tatctcttaa
     8701 gcgtagtaat tttagcggag gctgccagtt tcagcttacg cttttactct catttacgct
     8761 gctgatgctg gtatatgttg catttttagt ttagagttta cttaatttag ctaacactta
     8821 aatgactatg actgaactcg cacaattaca ggccagtgcc gaacaggcag cggccttatt
     8881 gaaagcaatg agccacccta aacggttgct gattctgtgc atgcttagcg gttcacccgg
     8941 caccagcgcg ggagagctga cgcgcattac cggactgagt gcctctgcga catcacagca
     9001 tctcgcccgt atgcgggacg aagggcttat cgacagccaa cgggatgccc aacgcattct
     9061 atattccatt aaaaatgagg cggtaaatgc cattatcgcc accctgaaaa atgtctattg
     9121 tccgtaagga gtcactatgg ctttgacaac catttcgccg catgatgcac aagaattaat
     9181 cgcacgcggc gcaaagttaa tcgatattcg tgacgctgat gaatatcttc gtgaacatat
     9241 tcctgaagca gatctggctc cattatccgt gctggaacag tcaggtctcc cggctaaatt
     9301 acgtcgcgag caaattatat tccactgcca ggcaggtaaa cgcaccagta ataacgccga
     9361 taaattagca gcgattgccg cccccgcaga aatcttttta ctcgaagatg ggattgatgg
     9421 ctggaaaaaa gcaggattgc cagtagcggt aaataaatct caacccttgc cgttaatgcg
     9481 ccaggtgcag atcgctgcgg gtggtttaat attaatcggc gttgtactgg gttataccgt
     9541 aaatagcggt ttcttcttat taagcggctt tgttggtgcc gggttactgt ttgcaggaat
     9601 tagcggtttt tgtgggatgg caaggttgtt agataagatg ccgtggaacc aatgagcttg
     9661 agaagcgacg ccggacgcgc catagcagcg acatccggcc tcagtaatta gatcaggaaa
     9721 tcgtcgagag atttaccttc tgccagcgcc tgagcgattg gcttcggtgt acggccctga
     9781 ccggtccagg ttttagtttc accgttaaca tcggtgaatt tatatttcgc cggacgcggc
     9841 tggcgttttt taccagcacg tggtgcagca gcagagctat tacccaataa ctcttccggg
     9901 ttaattccgt cagctttcat cagctccagc caggtgctaa ttttttcctg gcgctctgcc
     9961 agttcacgct gctgctgttc ttcttcttca cgtctttctt tagtgacaac cctgaatttt
    10021 tcgagcattt cttcaagaac gtcaatggag aattcgcgag ccatcgcacg gagggtgcga
    10081 atgttattta aactttgtaa cattacggac ataataaaga aaacctttta acgccaaaac
    10141 aaaaaagta
//
LOCUS       DAZGBC010000169        10029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGBC010000169 DAZGBC010000000
VERSION     DAZGBC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.170"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(600..1241)
                     /locus_tag="ACHK1J_004175"
     CDS             complement(600..1241)
                     /locus_tag="ACHK1J_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7913377.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISG
                     TKLDRPELGRLLMDSHRNDILLVEQIDRLTRLSNSDWITLKKQIEQHELRIVSLDVPT
                     SWQALSDKDPSQSDPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTLG
                     KYRGKQADQERHQKVLYYRQVKKLSIRETADATGYSTSQVCRIQALYREPTTD"
     gene            complement(1532..1990)
                     /locus_tag="ACHK1J_004176"
     CDS             complement(1532..1990)
                     /locus_tag="ACHK1J_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913378.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQRAARLAASV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
     gene            complement(2131..2979)
                     /locus_tag="ACHK1J_004177"
     CDS             complement(2131..2979)
                     /locus_tag="ACHK1J_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7913379.1"
                     /translation="MNNDELVTRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMRGKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            complement(3025..3289)
                     /gene="yacB"
                     /locus_tag="ACHK1J_004178"
                     /pseudo
     CDS             complement(3025..3289)
                     /gene="yacB"
                     /locus_tag="ACHK1J_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            complement(3289..3558)
                     /gene="yacA"
                     /locus_tag="ACHK1J_004179"
     CDS             complement(3289..3558)
                     /gene="yacA"
                     /locus_tag="ACHK1J_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO7913380.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            3767..3958
                     /locus_tag="ACHK1J_004180"
     CDS             3767..3958
                     /locus_tag="ACHK1J_004180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913381.1"
                     /translation="MERVLERSGEAPVYWPNLHASFRLREDAGFVPCVRLICSWPWHG
                     YGQILLRNSIGVPDLLKVP"
     gene            4191..4553
                     /locus_tag="ACHK1J_004181"
                     /pseudo
     CDS             4191..4553
                     /locus_tag="ACHK1J_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            4557..4912
                     /locus_tag="ACHK1J_004182"
                     /pseudo
     CDS             4557..4912
                     /locus_tag="ACHK1J_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            5193..6332
                     /locus_tag="ACHK1J_004183"
     CDS             5193..6332
                     /locus_tag="ACHK1J_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFO7913382.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            6452..7720
                     /locus_tag="ACHK1J_004184"
     CDS             6452..7720
                     /locus_tag="ACHK1J_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO7913383.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            7728..8873
                     /locus_tag="ACHK1J_004185"
     CDS             7728..8873
                     /locus_tag="ACHK1J_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO7913384.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            9341..9487
                     /locus_tag="ACHK1J_004186"
     CDS             9341..9487
                     /locus_tag="ACHK1J_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913385.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(9813..>10029)
                     /locus_tag="ACHK1J_004187"
     CDS             complement(9813..>10029)
                     /locus_tag="ACHK1J_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO7913386.1"
                     /translation="TAVSSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIE
                     HYNETHPHSALGYLSPREYRRQRVTST"
BASE COUNT         2418 a         2603 c         2443 g         2565 t
ORIGIN      
        1 caccctaaaa tatatggatt taaatgaagc aacccggatc acattttctc agaaaggtat
       61 cctttaaact ccaatgctaa tcgtgcaact gataacggct tccacaacag ggagccgttt
      121 tcttatgcca ctactcaagg atggggtttg agggccaacc gtacgaaaac gtacgatttc
      181 ggacagcgaa acagctgttg tcccgccatc cagacgattc ctgaaagtca tttgtctaaa
      241 aaatagtctt aacgtggttt attttccgaa ttccaagcgg ccccctggat gagcgtgatg
      301 attactggcg gacctttgac gttccagcaa attcccctgt tccacatagg tttccatata
      361 tgaccggtta ttttcacgtt cggtgtacgt gttacagctg caaataatac aggtgcatta
      421 tcaatatcat cagggataaa attgtaagat cgtggaaaat gtttattcat agcccggatg
      481 atggctgtct gtgcatcctt tgttcgggcg cggtgtttcc attcacgcca gccatccacc
      541 tcagaaataa taaggggtca gtttggatat cgaaaattat tgtacgttaa ggctactttt
      601 taatcagtcg ttggttctcg ataaagtgcc tgaatacggc aaacctgcga agtgctgtag
      661 cccgtagcat ctgctgtttc tctaatgctg agttttttta cttgtctgta gtacaacact
      721 ttctgatgcc gttcctgatc ggcttgtttt ccccggtatt tacccaacgt atgagctcgt
      781 tcaattccct gtttttggcg ttggcgacga ctcagccagt ccttatgcga catcgcggcc
      841 atcaggtcta taagcatatt atttattgca gttatcactg cgcgggtaat cggatctgat
      901 tgtgaaggat ctttatctga aagggcttgc catgatgttg gaacatccag actgacaatt
      961 cgcaattcat gttgttcaat ttgctttttc agcgttatcc agtcgctgtt gctaagacgg
     1021 gtcaggcggt ctatctgctc aaccagtaaa atatcattac ggtgactgtc catcagtaat
     1081 cgccccagtt ccggacggtc aagttttgta ccgcttatgt tctcccgata ataactggca
     1141 attttatgcc cccgttcctg gacgaactgt tccagcatct cttttgcccg atctgcaaac
     1201 tgatcttctg ttgatgccct taaataagct ctgatgaaca ttttttcacc gcattatcgc
     1261 atttaggttg ttgcatatat tctatcgcat ataggttatg tcattaattg tatgaggtca
     1321 cattttatat ctcgcatcag ggtgtaccct atcgcgacag gcaagcctgt tcaaaaaaca
     1381 ggcttgccgt acaataattc tctatatcca aactgacccc tatgagatca gtttggatat
     1441 tcgaaattat tgtacgttta acgcaccatc attaatgcta atgtatgcat acattagcat
     1501 taatggtgaa ggaaaagaat aacaatggca gtcaacccac ttcttttttg gatttcttaa
     1561 ttttcgtctg ataaagccgc tttacccgtt caatttctgc cagcgttgcg gaccagtgta
     1621 gttcggcggc atgacgatat ctggcagcca ttttttccac catctccgga ttgctgtccg
     1681 cgctgcggtg ctgacgcccc ttctccccaa aaacttcaac aaccagccac tggcttccgg
     1741 ttctgccgaa caatgccttg cacaaacgtc tcacagtcag cggggttaaa tctggatcgg
     1801 gatcataggc aatggtagca aaaataaagc gcagcatttc tgatgtcttg taacgcttta
     1861 cgctggcagc caggcgtgcg gcacgttgcg gtgcttccca gcggtcgtta ctgtaattag
     1921 ccacttcagc cagataatcc ttttcaagtt tttcagccgt gtagcacctg ccgcctaaaa
     1981 aaagccgcat ggcctccctg gttttatttc tcacatatca tccctcatta atgctaatgt
     2041 acgcatacat tagcattaac atactgacag taacaagcgg cggatttacg tccggataac
     2101 gtaaatgatt gcggtataca ggccaggcgg tcagatttcc agccgtgcct gttcctgcag
     2161 cagttccaga tgatatgcag caatagcgtt taccacccct gccgtcatcc gtgcgtcggc
     2221 gatcgctgag tgtgcctgcc cttcccaggt cagtccggcc tggctggcag cacaggccag
     2281 tgaaatcgtg ccatagcggt tggtggcccc ataatatcct gcagccagct ccatcgcaca
     2341 atataccgtc atttcttcca gccagtcagc cggatcgcta tgtgcggcag cagtctgttt
     2401 cagaatgcgg atgtcgaacc gggcattaaa gataattact ggtcggtccc cgattgcatg
     2461 ccgcagttga cgcgccacat ctgtccatga tggtgcgccg cagagagcct gctcactgat
     2521 accatgcacg gcggcagcct gcgccccgat ggcgaccgtg ggcttcagcc tggtttcaaa
     2581 taccacctga ccggccgcat cggtcaggcc gatttccagg gcttccgcgg tattatccag
     2641 tcctgtcgtt tcagtatcca gaaagagtgg tgccagactc agccagtcat atgcctgccg
     2701 tgccatccgg ccactggtgc tgtttagtcg ggataaaacg gaaagccgct ggccagccag
     2761 ctgttgttta ctcgtcagag ggcgtttccc acgcatcgga acacagtctg caattcggta
     2821 tacagcgaac ctgccaccat aagtattttt ataccactta accggctcag caccgggggc
     2881 tggcttcatt ctgaattcat cgcgcagacg tcctttcgag aaacacctgt cttcggcaat
     2941 cgcctgcgca cgtctggtga ccagctcatc attattcatg gttcttgcat ctccgtcaga
     3001 cgtttttata tccggacagc ccctctatgg ccacttctgt gcagtatgca atactcgcag
     3061 gatatacact tttccccact ggctgtcgac ctcataaacc agaacaaaat gcgggtgaat
     3121 aaccagttcc cgggtacctt ccacacgacc gttgcggcct ttatacggct gcccggccag
     3181 gctgctggct gaataaccaa tccgttccag ccatcaggtt ctgctccgca atgtactcac
     3241 gaatgcgctt cctgtcctgg ctggccagca tggtccagaa tatctccatc atttaccggc
     3301 aaccttcctg ctcatttcat cccgccatgc tgcagcagag gattcgacca tgtcatgggg
     3361 aagcacatcg ccgtgctcga gttgctgacg tcctgccgca acctggtcgc ggaaccaggt
     3421 attatgctgc cgttcaacgg tctgccgcat aaaatcccgg attaactgag agccattacg
     3481 gtccatgctt tttgcagcgg ccataaaagc atccttcagt tcagcgtcga ttcttaaact
     3541 catattaacc tgtgccattc atcacctcgt cattaccacg tatatacaca gtatataatt
     3601 attcagatat cgtcaccatg atatgccgcg ccagcaacat ggaaggcggc actccatgct
     3661 gtatatggca gaggagtaaa accgccgaat cccggcgtaa gccggtacag tcgtccgaat
     3721 gtcagtagtt ctactccccc ctgcgaggcc agtgctgacg cgggacatgg aacgggtgct
     3781 ggagcgaagt ggggaagctc cggtatactg gccaaaccta cacgcctcct tccgtctccg
     3841 ggaagatgct ggcttcgtac cctgcgtgcg tctgatctgt agctggccgt ggcacggcta
     3901 cggacagatt ctgctccgca acagtatcgg cgtgcctgac ctcctgaaag tcccgtaacg
     3961 cattttctca cctcccatgc cctgacgcta ttgagcgcgt tacagaacaa tttaagtcat
     4021 ttaacgagcc tccaacggct cgatcgaaat aaatcaaatc gtaatcgtca acgatatatg
     4081 agaggagacc ccgctcgccg caaatcgatg accgaacgta gtgagcgaat cgacgaggaa
     4141 gcggaagaga acctgtacga aaatggagca attacgcccc accgtcaaga aatgagctgg
     4201 ctctccacgg cgcgccatca tagacagatg tggctctgca actgcgccat gcgatcggag
     4261 accgaccagt aattatcttt aatgcccggt tcgacacccg cattctgaaa cagactgctg
     4321 ccgcacatag cgatccggct gactggctgg aagaactgac agtatattgt gcgatggaac
     4381 tggcagcagg atattatggg gccaccaacc gctatggcac gatttctctg gcctgtgctg
     4441 ccagcaggcc ggactgacat gggaagggct ggcacactca gctgatcgcc gacacacgga
     4501 tgacgtcagg ggtggtaaac gctattactg catatcatca ggaattgctg cagcttatgt
     4561 actgcgataa ccctgaactc aactatgcaa acctgaaatc cctgcaaaaa cagctggatg
     4621 aaatgtatac ctgcatccgt aataatcaga tgatgctccc aaactacgga aaaatgcgcc
     4681 ggtatggtga accggtttca acaacgtttg tagagtcgac aatcaacgaa gtgatcgcca
     4741 gacgatgacc caaaagcaac agatgcagtg gggtagggaa ggtacgcatt atttgttaca
     4801 gaccagaacc gctgtcctga ataattaact gcaggacaaa ttcgccagtt ggtacccggg
     4861 ttgcacgata gacgctcaga gtggaggaaa gttatccgcc atggcagcat aactacttcc
     4921 ccagtttttt cagctctccc ttaccaatat cctacgacga tcacatcgaa aaaaaacacg
     4981 gaacaggcga acagaaatat aattaattat ttgagtttaa aggaaaaaag aggatacaag
     5041 ctttttgttg tatttttatt tgtataaacc gacaaaaaag aggccatact tcccgctttc
     5101 tttgtctgtt tacacggaac atttcaccac tggatacgct aatagatcgg atatgagcac
     5161 atattgcgcc cgcctttcat gcagagcata aaatgaacaa gattcacgct aattattacc
     5221 tgcaagattc taccgcacgc cagatagttc aacgcacaat ggggattatt ccgtactcgg
     5281 taaatgtgat gaacgagcat gggatgatca tcgcttccgg ggaagcctca cgtttacatc
     5341 aacgccatga aggtgcggta ctcgcattga ccgaaaaccg ggtcgtcgaa attgattccg
     5401 cgaccgccaa taagcttaaa ggcgttaagc ccggaatcaa tttaccgatt aattttcgcg
     5461 accagctgat tggtgtactg ggcattaccg gcgagcctgg tgaagtgaag gcatatgcac
     5521 agttggtgaa gatggcttcg gaattaatca ttgaacaaat ggctctcctc gagcaaaaac
     5581 agtgggataa gcgttaccgc gaagagctga ttaatcagct gattttacgc gatccgccac
     5641 cggactcact gaatgcggtg gtctcatggt tgggaattaa tctccagctt ccgcgggtgg
     5701 tggtgattat ggaactgcaa caacctgatc gcgacgcact gcataatctg atggagtatt
     5761 ttgaatatcg cgcccgcgac catttggtca cgttttccga ctttaatgat ctgataatct
     5821 taaaacctat ctcgctaaaa aacggcgtct gggataaatc gttggaatta agcgatctgc
     5881 agagacttaa acattacgcc cagtcctgtg gtttttcccg actgattgtc ggcggctatt
     5941 tctctggaga aaacggcctg catcgctctt ttcagaccgc gaaatccacc cagtccatgg
     6001 cgaaaagact gaagttgaag catcagttca tttttcatga cgactatgcg ctggcatcat
     6061 tattagatgg gctttctggt acctggcaag cggaagaatt atccaaaatc tggcgcaatc
     6121 tggttgccaa ggacaccaaa ggtatgctgc aaaaaacgct gcagaattat tttgatcata
     6181 attgtgatct ttcgcaaaca gcgactggct tgcatattca tgtcaataca ttacgctatc
     6241 gactgcagag agtggaagat attacaggaa tacaaatcaa taacttaaag cagttattct
     6301 ggctgtatat cggcatggag ttacaacgct gaactgtatt tccctccaaa tttttcgccg
     6361 tctgacgtcg tatttttagc cgatacacac aagtctaacg cggctgcttt ctccatactg
     6421 ccccctgtta ttttcatgga gaaagcagaa gatggtttca ataacagcaa ctggcgccat
     6481 catcgcactc gtggtggcga tcgttttaat tttacgcaaa gtctcgccgg cttacggcat
     6541 gattgtcggt gcgctggtag gcggtctggt cggcggcgcc gatctggtgc aaaccgtgac
     6601 gctaatggtc actggcgcgc aaggcattac caatgccgtg ctgcgcattc tcgcagcagg
     6661 tgtactggcc ggcgtgttga tagaatccgg cgcggcaaac accatcgctg aaactgtggt
     6721 gaaaaaagtg ggggaaagcc gggcgctttt agccttagct attgcgacga tgattctgac
     6781 cgccgtcgga gtatttatcg acgtggcggt tatcactgtt gcgccgattg ccctgtctat
     6841 tgcccgtcgt gccggtttgt cgaaactcgc cattctgctg gcgatgattg gcggtggtaa
     6901 agcgggtaat gttatgtcgc ctaaccccaa tgctatcgcc gtttcggaca cctttcatgt
     6961 tcccctcacc tccgtgatga tgaccgggat aatccccggt ctgttcggcc tgatcgtggc
     7021 ttatttcctg gcaaaatgtc tcagccagaa aggcctgatg gtgatgcccg acgaaatgat
     7081 ttcacacgac aaccacggca agcagccggg atttcttgcc gctatcagcg cgccgctcgt
     7141 cgccattgtg ctgctggcgc tacgacctgt tgcgggtatc gtcatcgatc cgctgattgc
     7201 cctgcccgtc ggtgggttag ccggtgcgat ccttatgggc aggtttaagc aaagtaacgc
     7261 ctttatgatg tccgggctta cgcgcatggc gccggtagcc atcatgctgc tcggtaccgg
     7321 tacgctcgcc gggattatcg ccaactcgga actgaaagat gtgctcattc atggactgac
     7381 ctcttccggt ctgccttctt atcttctggc ccctgtttcc ggcgcgatca tgtccatggc
     7441 aacagcctcc accacggcgg gaaccgctgt tgccgccggg gtattcagcc agacgctgct
     7501 ggggcttggc gtatcagccc ttgccggttc cgccatgatt catgctggcg caaccgtact
     7561 tgatcacctg cctcacggca gcttcttcca cgccacgggc ggcagtgtcg gcatgctgat
     7621 ccatgaacgg ttaaaactgc tgccttatga aacccttgtc ggttttgtgg tcgccctgat
     7681 ttccacactg atgtttggcg tcttcggact tgccgggtaa agacacgatg aaaatagtca
     7741 ttgcaccaga ttcatttaaa gaaagtttat cggccatgca ggtggccacc gctattgagc
     7801 agggatttcg tgaaatttat cccgaggccg agtatgtcaa attgccgatg gctgacggcg
     7861 gtgagggtac cgtcgaatca atggttgaag caaccgaagg ccattatctg ttccagaatg
     7921 tcaccggacc gctggggacg ccagtacagg cgcgctgggg tatgctggga gatgggaaaa
     7981 cggcgatcgt tgaaatggcc tccgcatccg gactacatca tgttcctcca gaatgccgta
     8041 accctatgct taccaccagt tatggcacag gcgaactgat ccttgccgca ctggaccacg
     8101 gcgttgagcg aattattctc gggattggcg gtagcgccac caatgacggc ggcgcaggta
     8161 tgctacaggc acttggtgtg gcgatgcttg atacaaacgg gaaatcgctt cctttcggcg
     8221 gcggcgcgct aagcaacctg gcgctcatcg atgcctcagg ctgcgacgct cgactgaaaa
     8281 acgtttctgt caccgtcgcc tgcgacgtta acaatccact ctgtggcgca acaggagcgt
     8341 cagccatctt cggcccgcaa aaaggagcaa caccagaaat ggttgcctcc ttagaagctg
     8401 cgctgaccaa cttcggtata cgacttcagg acgcaaccgg acgaaacgtg atgacgacgc
     8461 ctggcgcagg tgccgctggc ggcatgggcg cggcgctgtt agggattctg aatgcggagt
     8521 taaagccggg agtggagatt gttattgacg cgctgcatct cgatagcctg gtcgcggatg
     8581 ccagcctggt aatcaccggt gaaggtcgtc ttgatagcca gagcatcagt ggtaaaacac
     8641 cgattggtgt cgcacgctgc gctaagcggt atcagaaacc ggtgatcgca ctggcgggcg
     8701 gcttaacgac cgaccaccag atagtgcatg agcacgggct ggacgcggtg ttctccgtgc
     8761 tgacgcaaat cgccactctg ccggaagcgc tacaggatgc ggaatacaac ctccgcgttt
     8821 cggcacgcaa tattgccagc gtctggaaat tatcacagca gcagatgcgc taatcttcag
     8881 tcaggccggc aagcagtagc tggcctggat attctttctc accaggccca tgctaaaaca
     8941 gcctgcttca gtcatcccac gcaaggcact agcatggcaa aaaaagaggg gagtagtgga
     9001 tcccaaaaga cggttaagcg gttaaattga atggtcaata agggggctgt ttgggaaacg
     9061 aatctatggt cactcccgtt tttgcaacac cgattttgac gataagtggg cttgcttgaa
     9121 tctatccggc gtctgaatgg gattttattc ccgcgcctcg atgagttccg cgcctgatga
     9181 acctcaagaa aatatacggc ttcaatgagc ctttccgttt cacaggttcc tcaacaggcc
     9241 ggtgggccgt tcatatcatc aataccgtga tgctgatact ccgtacccac cggaaccgca
     9301 cctgatgcgg ttccggctta agggctgtca ggactcgctt ttggctgcca tcaacaaacc
     9361 ggaagagatg ccggattatc tcttccgtca actcggtttt aatcagacct ggcatgagtg
     9421 gaagcgggac gaacagcaca ggcaacaaca acgccggccc ggacactacc ggggcatgag
     9481 tatgtaatgt cagggacagc tatacgccat aaaaaaatga aatagatggc ctctctataa
     9541 agaggccatg atatttacta accctgttcg ttaatacatt ctataaagtt tttatgtagg
     9601 ctatctatat tttctcgggt atgaatagct gatattgcac aacgtagaat tgcctttccc
     9661 tcttccactg taggatacat agcaacggtt gtagcaaaac cacgatcatg catttttttg
     9721 catatcgtta tagctttttc ttcattgcca attaacaatc cacgtattgg cattgtgtcc
     9781 ttgacccacg atattcagac agtcgttgta tcttaagttg acgttacccg ctgacgtcga
     9841 tattcccttg gtgagagata acctaacgca ctatgcggat gggtttcatt gtaatgctcg
     9901 atcgccactg caaggttatg caatgccgtt cttacattcg gtttcggcat gaacgcgatg
     9961 tagtcttctt tcatcgtttt cacgaacctt tctgccattc cattgctctg agggctactg
    10021 actgccgtc
//
LOCUS       DAZGBC010000170         9983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGBC010000170 DAZGBC010000000
VERSION     DAZGBC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.171"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..269
                     /gene="hokA"
                     /locus_tag="ACHK1J_004188"
     CDS             117..269
                     /gene="hokA"
                     /locus_tag="ACHK1J_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO7913387.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(246..317)
                     /locus_tag="ACHK1J_004189"
     CDS             complement(246..317)
                     /locus_tag="ACHK1J_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913388.1"
                     /translation="MRNAVRKAGITSRRRLLLFQIAG"
     gene            complement(457..669)
                     /gene="cspA"
                     /locus_tag="ACHK1J_004190"
     CDS             complement(457..669)
                     /gene="cspA"
                     /locus_tag="ACHK1J_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO7913389.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(950..1240)
                     /gene="yiaG"
                     /locus_tag="ACHK1J_004191"
     CDS             complement(950..1240)
                     /gene="yiaG"
                     /locus_tag="ACHK1J_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO7913390.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1674..2384
                     /gene="yiaF"
                     /locus_tag="ACHK1J_004192"
     CDS             1674..2384
                     /gene="yiaF"
                     /locus_tag="ACHK1J_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO7913391.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2434..3408)
                     /gene="ghrB"
                     /locus_tag="ACHK1J_004193"
     CDS             complement(2434..3408)
                     /gene="ghrB"
                     /locus_tag="ACHK1J_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO7913392.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALMLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALLGKVEKNCVNPHVAD"
     gene            complement(3512..4171)
                     /gene="yiaD"
                     /locus_tag="ACHK1J_004194"
     CDS             complement(3512..4171)
                     /gene="yiaD"
                     /locus_tag="ACHK1J_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO7913393.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4324..6657
                     /gene="bisC"
                     /locus_tag="ACHK1J_004195"
     CDS             4324..6657
                     /gene="bisC"
                     /locus_tag="ACHK1J_004195"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO7913394.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATEMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLEMFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6626..7066)
                     /gene="yiaC"
                     /locus_tag="ACHK1J_004196"
     CDS             complement(6626..7066)
                     /gene="yiaC"
                     /locus_tag="ACHK1J_004196"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7913395.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7063..7626)
                     /gene="tag"
                     /locus_tag="ACHK1J_004197"
     CDS             complement(7063..7626)
                     /gene="tag"
                     /locus_tag="ACHK1J_004197"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO7913396.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7784..8482
                     /gene="yhjY"
                     /locus_tag="ACHK1J_004198"
     CDS             7784..8482
                     /gene="yhjY"
                     /locus_tag="ACHK1J_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO7913397.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGSWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8711..9913
                     /gene="yhjX"
                     /locus_tag="ACHK1J_004199"
     CDS             8711..9913
                     /gene="yhjX"
                     /locus_tag="ACHK1J_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7913398.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLIISLALSTTIRQPQQ
                     KVLREAHI"
BASE COUNT         2373 a         2425 c         2579 g         2606 t
ORIGIN      
        1 cgaaaggcag acagagaaaa gccccacctg actataaatc aaagtgaggc ccctctatgc
       61 atagacacca taaggttagc ctcttactcg ttggaaatca acacaggggg ctgggaatgc
      121 cgcagaaata tagattgctt tctttaatag tgatttgttt cacgctttta tttttcacct
      181 ggatgataag agattcactg tgtgaattgc atattaaaca ggggagttat gagctggcgg
      241 catttttagc ctgcaatttg aaagagtaag agtcttcggc gggaagttat tcccgccttt
      301 cttacggcgt tgcgcattct cattgcaccc aaatttattt ttcacaaaaa taatactaga
      361 ttttattacg cgatcgatta tttatttcct gaaaaacaaa taaaaaaatc cccgccaaat
      421 ggcagggatc ttagattctg tgcttttaag cagagattac aggctggtta cgttaccagc
      481 tgccgggcct ttagcgccgc tttcgatggt gaaggacact ttctgacctt cgtccagaga
      541 tttgtaacca tcgttctgga tagcagagaa gtgtacgaac acgtctttag agccatcgtc
      601 aggagtgatg aagccgaagc ctttgtcagc gttgaaccat tttacgatac cagtcatttt
      661 accggacata gtgtattacc tttaataatt aagtgtgcct ttcggcgata tggcgtgctt
      721 tacagatttt gaagcgttaa aggaatgtgc actacgaggg gtatcaacga taactcttga
      781 agggacttgc cttactacac tgctttaatg gtctgtacgt caaaccgttg atgtgcatta
      841 agccacgcat tggcgggtga tgcaacaatt atttttcata tttatgatta atcggttgga
      901 aaacggtcct gtcatcagga ccgtaaaccg caataaagtg gataaaagtc tattccatca
      961 actgcttact taatgccggg ttggcttgaa tcaaacgcat caattttagt tcggcacttg
     1021 aaggcttcac gcgtctggat tcccattcct ttaccatggc gactgatacg cccaaaaccc
     1081 gggcgaaatc atcgattttt aatcctgtcc cttttcgtaa ctgctcaatt tcggtacagg
     1141 acgttgttct gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca atctgttcca
     1201 ggctgctcaa cagctcatgc attggatctt tatattccat tgaaaactcc tcaaatcaca
     1261 ctgcgggatc gtgaattgca tcgaagctca ttaagaatag tcgggaaaag cagacacagc
     1321 ggggtgaagt cagggcatgc tctggctatg attcgattca gccagtcatt tagcgtgact
     1381 tatagccacc taacgatctg ataaatgccg atatatctct taccactcct ggtttgtaaa
     1441 gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct ggctacaaaa aaggcaacgg
     1501 caatatcatt ttttaacaat gaaacaacaa attaaccttg tttcattttc tccatgattt
     1561 taccaggaag attacgtaga aagggtatcc tgcaagcctg tcctggacta tccttgtcac
     1621 gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag aaaatggcga
     1681 caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg gtagttagcc
     1741 tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac ttcctgcaga
     1801 atacagtgat gcgtagcggt gaacgtctac caaccctgac tgccgatcag aaaaaacagt
     1861 ttggtccttt tgtctctgat tacgcgattc tgtatggtta ttctcagcag gtgaatcagg
     1921 cgatggattc cggtctgcgc ccggttgtag acagcgttaa cgcaattcgc gtaccgcagg
     1981 attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg ttcgctgggt gtgctggcgc
     2041 aacaactgca aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta aaacagagtg
     2101 atgacctgaa accggtcttt gatcaggcat tcaccaaagt ggtaacgacg ccagctgatg
     2161 cattgcaacc attgattccg gcggcacaaa ctttcacgca acaactggtg atggttggag
     2221 actacattgc ccaacaaggt actcaggtga gctttgtagc aaatggcatc cagttcccga
     2281 cttcacagca ggcaagtgaa tataacaaac tgattgcgcc attaccggca cagcatcagg
     2341 cgtttaatca ggcctggact acagcagtta ctgcaaccca ataaagagta aaagcccgag
     2401 tgattatcgg gctttactcc acgcagtcgc ggcttagtcc gcgacgtgcg gattcacaca
     2461 gttcttctca acctttccta gtaacgcatc aatcaaatta tccacggcac aggcggccat
     2521 gccataacgc gtctcatggg tggcagatcc aatatgcggt actgcgacga cgttggccat
     2581 tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg aagacatcca gcccggcggc
     2641 gtgaatttcg cctttctgca atgcagcaat cagtgcattt tcatcaacca ccgggccacg
     2701 tccggcatta atgaatatgg cggaggattt cattttggcg aattgttctg cgccaaatag
     2761 atgatacgtc tcatcagtta acggcaggat caggcaaacg aaatctgact cttgtaacag
     2821 tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct gcttctttat ggtggcggcg
     2881 cgcgttatag aggatgggca tgttgaagcc aaagtgcgca cgttgtgcca gcgccatacc
     2941 gatccgtccc atcccgacaa tgcccagtgt tttatggtga acgtcagtgc cgtaccagtc
     3001 cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt tctgctacct ccacaacccg
     3061 acgagcggta gacaacatca gcgccatcag cgtatcggcg acggtttctg ttaatacggt
     3121 tggcgtgtgc atgagcagaa tttttcgggc ggtaagcgca tcgacatcaa aattgtcata
     3181 gccgacggag atcgttgatg tggcacgcag tttcggcatt ttttccagca atgcggcatc
     3241 tacattctcg tttgaaccca gtaaaccttc agcttcggca aaaattgctg cattttgatc
     3301 gacggtttgt gggctgaggt ttgccacctg gtgaacggtg aaatgctctt gcaggcgttg
     3361 cagtaaatca tcaggtaagg ctttgtagag gataacggac ggcttcatgc ttctctccat
     3421 tcactgataa ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga agaatgccag
     3481 gtgatttctg catcacctgg catgaaaggg attacagcgg gcttaaggta atttctacac
     3541 ggcggttttg cgccttacct tctgcggtgc tgttgctggc gattgggtta gccgggccaa
     3601 ggccctgagt acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg ctggcaacgg
     3661 aatccgcacg ctgttgggag agacgcatgt tcagatcgtg accacccgtg ctgtcggtat
     3721 aaccaatcac gttaaccgcc gttttcggat actctttcag caccattgcc acgccggtca
     3781 gggtgttagc gcccgcaggt ttcagggtcg cgctgctgct gtcgaaggtc acattgttcg
     3841 gcatattgag gataatgtta tccccgctgc gggttacact aacaccagtg ccgcgcattt
     3901 tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc cacgccgccg cccagagctg
     3961 cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc tttcttcgaa gaagagagcg
     4021 caccaatacc cgcgcccacg agagagccca gacctgcgcc gatagcagat ttacctgctt
     4081 cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac cgccagagcg ccactcacta
     4141 cggcggcaat aagataaaca cgtttcttca ttgttaatcc ttaataacct ttttattctt
     4201 tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca tgaagattat tcctgggaat
     4261 actcggaaat ttgtaagtaa tatttaactg ctcaatacat ctaacctttc aggagtcttc
     4321 ggtttggcca actcatcctc acgatattcc gttctgactg ccgcccattg ggggcccatg
     4381 ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc cacagaaatg
     4441 gaaaactcct tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg ggtacgattt
     4501 ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat tcgtgggcag
     4561 gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca acaacataaa
     4621 cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg ctggcgttca
     4681 aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct ggcaggcggt
     4741 tataccgggc atctggggga ttattcgacc ggcgcggcac aggcgatcat gccgtatgtc
     4801 gtgggtggta gtgaagttta tcaacagcag accagttggc cgctggtgct ggaacatagc
     4861 gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc tgaaaattgc gtggaatgca
     4921 tccgatgagc aggggctttc ttacttttct gcactgcgtg acagcgggaa aaagctgatc
     4981 tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat ggagtgggtg
     5041 gcaccgcaca tgggcactga tgttgcgctg atgctgggga tcgcccatac gctggtggaa
     5101 aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc cgtcttcgcc
     5161 tcttatttgc tgggcgagag tgacggaata gcgaaaaccg ccgaatgggc agcagagatt
     5221 tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca aaataccacc
     5281 atgctgatgg ctggctgggg aatgcagcgc caacagtttg gtgagcaaaa acactggatg
     5341 atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg ttttggtctt
     5401 tcttaccatt ttgccaatgg tggtaacccc acgcggcgcg ctgcggtact ctcttccatg
     5461 cagggcagct tgccgggtgg ctgcgatgcg gtagataaaa tccctgtcgc ccgcattgtt
     5521 gaagcactgg aaaaccccgg tggcgcatat caacacaacg gtatggaccg acatttcccg
     5581 gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca ggataccaac
     5641 cgcctgatcc gtgcctggca aaaaccggag ctggtggtga tctccgaatg tttctggacg
     5701 gcggcggcga aacacgcgga tatcgttctg cctgcgacta cctcttttga gcgtaatgat
     5761 ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca agtggtgccg
     5821 ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttaagtga acgctgggag
     5881 aagggcggct atgcgcggtt tacggaagga aaaagtgagc tgcaatggct ggaaatgttt
     5941 tataacgttg cccgacagcg cggggcaagc cagcaggttg aattgccgcc atttgctgag
     6001 ttctggcaag ccaatcagtt aattgagatg ccggaaaacc cggacagcga gcggtttatt
     6061 cgcttcgctg atttttgccg cgatccgcag gcgcatccat taaaaaccgc cagcggtaag
     6121 attgaaatct tctcgcagcg tattgccgat tacgcttatc cggattgccc ggggcatcca
     6181 atgtggctgg agccggacga atggcagggt aatgccgaac cggaacagtt acaggtactt
     6241 tctgctcatc cggcgcatcg tctgcacagc cagctgaatt acagttctct gcgcgaattg
     6301 tacgcggtag caaaccgtga gcctgtgacc attcatcctg acgatgctca ggcgcgcggc
     6361 atacaagatg gcgatactgt tcggctgtgg aactcacgcg ggcaaattct tgccggagcg
     6421 gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc atggccggat
     6481 ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct gaccaaagat
     6541 ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc atggctagaa
     6601 aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag ctcataatcc
     6661 aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa ccctgcgcct
     6721 ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga tggggatggc
     6781 gctgttgcac atactgcatc agcgccttac caataccgcg cctgacggcc ttcggtgcga
     6841 caaacatcgc tgccagaaat cggccttcca taatgctgac aaaaccaaga agcttactgt
     6901 cttcttccca gacccagttt tgtgcgttgg caagataggc atcccgcacc agcggaatgc
     6961 actcgcgcca gtaattcgct tttataaagg gatggcccca ggttgtactt tccagccaca
     7021 gttcgaggat cgcggggagt tctgaacgtt gcgcttccct aatcatggtt tatttcccgg
     7081 atagcaacag cagccaacca catgatcatt caccagccca catgcctgca taaaggagta
     7141 acagattgtg gtaccgacaa acttaaaacc acgttttttc agtgccttag atagggcgtc
     7201 ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg gcttgtgtca cctgtggctg
     7261 atgatttaca aacgaccaga caaagtcggc aaacggttcg ccgttctgtt ccatttgcag
     7321 gtacgcccgc gcattaccaa taattgcctg aattttcccg cgatggcgga taatcccggc
     7381 gtcctgtacc agtctttcga catcctcttc ctgcattgcg gcaaccttca ccggatcgaa
     7441 ctgatgaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc acgataaccc
     7501 agcttgctgc ccttccaggc agatcatttc gaacagtttt ttactgtcag tttcaggcac
     7561 gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc agccgcaacg
     7621 ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt ttcttgacgc
     7681 atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct tcatacaatg
     7741 acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca taaaaaaaag
     7801 cggtggtcgc tggcagctaa gcctgctggc gagtgtggta atcagtgcct tttttctcaa
     7861 cacagcttac gcctggcaac aagaatatat cgttgatacg caacccgggc attccacaga
     7921 gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt cgcaacgtat
     7981 tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa cccctgtgga
     8041 tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg ttacaactgg
     8101 cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg agtttggcga
     8161 cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtacct taggctggcg
     8221 tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata accagcaatt
     8281 tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga caaaccagaa
     8341 cggcagctgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa atattgccgc
     8401 ctatgccgcg ctatctcagg cagaaaatac cactaacaat agcgattatc tgtatacgat
     8461 gggggttagt gccagatttt aacgtaacaa tcacaattga aaccattgaa taacaatagt
     8521 tgtggcgata gtgggtgcta acttaccaaa taataaattt ggtgaataat tgtcgcgtca
     8581 ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc gtttttcccc
     8641 aggcataaag tgcacttcgt tatggttgtc ggcagagatt tttccttttt attactgcag
     8701 gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac tcatcggtac
     8761 tatcattacc cagtttgcgc tggggtcggt ttatacctgg agcctgttta acggcgcgct
     8821 ttccgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct tgttaagtct
     8881 ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg gtgttaaacg
     8941 cgtcaccatc gcttccggca ttttgctggg attagggttc ttcctgacag cgcattctga
     9001 caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg atggcgcggg
     9061 ttacctgctg acgctctcta actgcgtgaa atggttcccg gagcgtaaag gtctgatctc
     9121 cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta tcgacacgca
     9181 gctgctggaa acggtcggtc tggaaaaaac ctttgtgatt tggggagcga ttgcgctggt
     9241 gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag tgaaaaccag
     9301 caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac cgcagtactg
     9361 gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga ttggggtagc
     9421 gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tctgcagcca atgcagtcac
     9481 tgttatttcc atcgccaacc tttcaggtcg tctggtgctg ggcattctgt ctgacaaaat
     9541 cgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta tggcggccct
     9601 gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg attgcctgtg tggcatttaa
     9661 ctttggcggc actattaccg tctttccgtc actggtcagt gagttcttcg gcctcaataa
     9721 cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca tttgtggttc
     9781 gattatcgcc tcactgtttg gcggcttcta tgtgacgttc tacgtcatct ttgctctgct
     9841 gattatttcc ctggcacttt ccaccaccat tcgccagccg cagcagaaag tgttgcgtga
     9901 ggcgcatatt taatgagatg gcgttggtta ttaacgaagg ggcgagagag cccctttttt
     9961 tcgtttaaaa taataaggag att
//
LOCUS       DAZGBC010000171         9852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGBC010000171 DAZGBC010000000
VERSION     DAZGBC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.172"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            217..825
                     /locus_tag="ACHK1J_004200"
     CDS             217..825
                     /locus_tag="ACHK1J_004200"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO7913399.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            923..2314
                     /gene="selA"
                     /locus_tag="ACHK1J_004201"
     CDS             923..2314
                     /gene="selA"
                     /locus_tag="ACHK1J_004201"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO7913400.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2311..4155
                     /gene="selB"
                     /locus_tag="ACHK1J_004202"
     CDS             2311..4155
                     /gene="selB"
                     /locus_tag="ACHK1J_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO7913401.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4345..5496
                     /gene="yiaY"
                     /locus_tag="ACHK1J_004203"
     CDS             4345..5496
                     /gene="yiaY"
                     /locus_tag="ACHK1J_004203"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO7913402.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            5661..7199
                     /gene="aldB"
                     /locus_tag="ACHK1J_004204"
     CDS             5661..7199
                     /gene="aldB"
                     /locus_tag="ACHK1J_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO7913403.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAYTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            7744..8067
                     /locus_tag="ACHK1J_004205"
     CDS             7744..8067
                     /locus_tag="ACHK1J_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7913404.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            8073..9209
                     /locus_tag="ACHK1J_004206"
     CDS             8073..9209
                     /locus_tag="ACHK1J_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7913405.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(9206..>9852)
                     /locus_tag="ACHK1J_004207"
     CDS             complement(9206..>9852)
                     /locus_tag="ACHK1J_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7913406.1"
                     /translation="SKHVIDKIKDISDITLSLKTCNLERNAFYEGVEFPDDCDVLISY
                     APPKDESLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWR
                     FNVAGSKEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQ
                     HPYEWWLDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2371 a         2394 c         2706 g         2381 t
ORIGIN      
        1 gtattatcgg gttatttgtc cgctgacagg caaaacccta cagatttata agaaagcctt
       61 aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatcacc atgataaata
      121 tgtgatttat tgggaggtga caaaccatca acattttctg ccctttttcc catcaccagg
      181 ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg tagctacacc
      241 agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcattac tttcgaattt
      301 attaatgaac tgccctataa cgcggacaat ggcgtggcgc aatttaaccc gttagggaaa
      361 gtgccagtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat catcgccgaa
      421 tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccttt ggagtcgttg
      481 cgggtgcgca aaattgaggc gctggccgac ggcattatgg atgctgggct ggtgtcggtg
      541 cgtgaacagg cgcgtccggc ggcgcagcag tctgaagatg aattgttacg ccagcgggag
      601 aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac acttaaaacc
      661 gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct caatttccgc
      721 cgcgttgcgc caggctggtg tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg
      781 tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct
      841 cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc gtgtgagagg
      901 gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata gtcaacttcc
      961 ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata cttatggtca
     1021 cacccgcgtg gtggaattgc tgcgtcagat gcttgacgaa gcgcgagaag tgattcgtgg
     1081 cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac
     1141 gaaagaggcg cagagcgcgc tgcgtccggt gataaacctg acgggaaccg tgctgcatac
     1201 caatcttggg cgagctttac aggcggaagc tgcggtggaa gccgttgcgc aggctatgcg
     1261 ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc gcgatcgggc
     1321 gctggcgcag ctgctgtgcc gcattaccgg cgcggaagat gcctgtatcg ttaataacaa
     1381 tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg tggtggtttc
     1441 tcgcggcgaa ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc
     1501 aggctgcaca ctacacgaag tagggaccac caaccgcacg cacgcgaatg attatcgtca
     1561 ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact acagcattca
     1621 ggggttcacc aaagcgatag acgaagctga actggtggcg ctcggcaaag agctggatgt
     1681 tccagtggtg actgatttag gcagtggctc gctggtcgat cttagccagt acggtttgcc
     1741 gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga gtttctccgg
     1801 cgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag agatgatcgc
     1861 ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga cccttgcggc
     1921 gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa aattaccgac
     1981 cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac gtttacaggc
     2041 tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat gtctttcgca
     2101 gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa cgtttacacc
     2161 ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg aattgccagt
     2221 gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct gccttgaaga
     2281 tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg acacgttgac
     2341 cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgccgatcg tctgccggaa
     2401 gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca gccggatggt
     2461 cgtgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa catgctggcg
     2521 ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggca
     2581 cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct gacagtggcg
     2641 ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttaatg aggttgaacg ccaggtaaag
     2701 gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa
     2761 ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccagaacg tgaacacgcc
     2821 agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg tgccgggctg
     2881 gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact atggctgact
     2941 ggtgtaaata aaccgatgcg cgtgcgtgcg ctgcatgcgc aaaaccagcc aacagaaacc
     3001 gctcatgccg ggcagcgtat cgcgcttaac atcgcggggg atgcggaaaa agagcagatt
     3061 aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacgcg ggtgattgtc
     3121 gagcttcaaa cccatacgcc gctgacccag tggcagccgc tgcatattca ccacgccgcc
     3181 agccacgtta cgggacgcgt ttcgctgctg gaagataacc ttgccgagct ggtgttcgac
     3241 acgccgctgt ggctggcgga taacgatcgc ttagtgttgc gcgatatctc ggcccgcaat
     3301 acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg
     3361 gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga tgcgttatct
     3421 gttcatctgg aacgcggagc ggttaacctt gcggatttcg cctgggcgcg ccagctcaac
     3481 ggcgaaggga tgcgcgaact gctgcaacag ccgggctata ttcaggctgg ttatagcttg
     3541 ttgaatgcgc cggttgccgc ccgctggcag cggaaaattc ttgacacatt agcgacttat
     3601 catgagcaac atcgcgatga acctggtcct gggcgcgaac gtctgcgacg tatggcgttg
     3661 ccaatggaag atgaagcgct ggtactgttg ctgattgaaa agatgcgcga aagcggcgac
     3721 atccacagcc atcacggctg gttgcatctg ccagatcaca aagcgggctt cagcgaagag
     3781 cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt
     3841 gacctggcaa aagagacggg aaccgacgag caggcaatgc gtctgacgct acgccaggcg
     3901 gcccaacagg ggataattac cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt
     3961 gtcgagtttg ccaatatgat ccgcgatctc gatcaggaat gtggttcaac ctgcgcggcg
     4021 gatttccgcg atcgcttagg ggttgggcga aaactggcga ttcaaatcct ggaatatttt
     4081 gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga cgcattatta
     4141 tttccggaaa aataaggaaa tgattaatta agttttaaaa taaattaata caaaattctt
     4201 atgattttaa aaaaagcaca ttgtttaatg aatacaatgt gctttttatt agattaattt
     4261 tgctaaacaa taatactgat catattttcg aatgaaaatc ctcagtaagc tgcccgccct
     4321 tttttacact ttcaggagtg tgttatggca gcttcaacct tttttattcc ttctgtgaat
     4381 gtcatcggcg ctgattcatt ggctgatgca atgaatatga tggcagaata tgggtttcgc
     4441 cgtacattga ttgtcactga cagtatgtta acgaaattag gtatggcggg cgatgtgcaa
     4501 aaagcactgg aagaacgcaa tatttttagc attatttatg atggtaccca acctaacccc
     4561 accacggaaa acgtcgccgc aggtttgaaa ttacttaaag aaaataattg cgatagcgtg
     4621 atctccttag gcggtggttc tccgcacgac tgcgcaaaag gtattgcgct ggtggcagcc
     4681 aatggcggcg atattcgcga ttacgaaggc gttgaccgct ctgcaaaacc gcagctgccg
     4741 atgatcgcca tcaataccac ggcgggtacg gcctctgaaa tgacccgttt ctgcatcatc
     4801 actgacgaag cgcgtcatat caaaatggcg attgttgata aacatgtcac tccgctgctt
     4861 tctgtcaatg actcctcgct gatgatcggt atgccgaagt cactgaccgc cgcaacgggt
     4921 atggatgcct taacgcacgc tatcgaagcg tatgtttcta ttgccgccac gccgatcact
     4981 gacgcttgtg cactgaaagc cgtgaccatg attgccgaaa acctgccgtt agccgttgaa
     5041 gatggcagta atgcgaaagc gcgtgaagca atggcttatg cccagttcct tgccggtatg
     5101 gcgttcaata atgcttctct gggttatgtt catgcgatgg cgcaccagct gggcggtttc
     5161 tacaacctgc cacacggtgt atgtaacgcc gttctgctgc cgcatgttca ggtattcaac
     5221 agcaaagtcg ccgctgcacg tctgcgtgac tgcgccgctg caatgggcgt gaatgtgaca
     5281 ggtaaaaacg acgcggaagg tgctgaagcc tgcattaacg ccatccgtga actggcgaag
     5341 aaagtggata tcccggcagg cctacgcgac ctgaacgtga aagaagaaga tttcgcggta
     5401 ctggcgacta atgccctgaa agatgcctgt ggctttacta acccgatcca ggcaactcac
     5461 gaagaaattg tggcgattta tcgcgccgca atgtaatcat catttccaca acggctggca
     5521 aattgtcagc cattttttca actatctctg taatccttgc ccgtaaattc gtgatagctg
     5581 tcgtaaagct gttaccgact ggcgaagatt tcgccagtca cgtctaccct tgttatacct
     5641 cacaccgcaa ggagacgatc atgaccaata atcccccttc agcacagatt aagcccggcg
     5701 agtatggttt ccccctcaag ttaaaaaacc gctatgacaa ctttattggc ggcgaatggg
     5761 tagcccctgc cgatggtgag tattaccaga acctgacgcc ggtgaccggg cagctgctgt
     5821 gcgaagtggc gtcttcgggc aaacgagaca tcgatctggc gctggatgcc gcgcataaag
     5881 tgaaagataa atgggcatat acctcggtgc aggatcgcgc ggcaattctg tttaagattg
     5941 ccgatcgaat ggaacaaaac ctcgagctgt tagcgacagc tgaaacctgg gataacggca
     6001 aacccattcg cgaaaccagt gctgccgacg tgccgctggc gattgaccat ttccgctatt
     6061 tcgcctcgtg tattcgggcg caggaaggtg ggatcagtga agttgatagc gaaaccgtgg
     6121 cctatcattt ccatgaaccg ttaggcgtgg tggggcagat tatcccgtgg aacttcccgc
     6181 tgctgatggc gagctggaaa atggctcccg cgctggcggc gggcaactgt gtggtgctga
     6241 aacccgcacg tcttaccccg ctttctgtac tgctgctaat ggaaattgtc ggtgatttac
     6301 tgccgccggg cgtggtgaac gtggtcaatg gcgcaggtgg ggaaattggc gaatatctgg
     6361 cgacctcgaa acgcatcgcc aaagtggcgt ttaccggctc aacggaagtg ggccaacaaa
     6421 ttatgcaata cgcaacgcaa aacattattc cggtgacgct ggagctgggc ggtaagtcgc
     6481 caaatatctt ctttgctgat gtgatggatg aagaagatgc ctttttcgat aaagcgctgg
     6541 aaggctttgc actgtttgcc tttaaccagg gcgaagtttg cacctgtccg agtcgtgcct
     6601 tagtgcagga gtcaatctac gaacgcttta tggaacgcgc catccgccgt gtcgaaagca
     6661 ttcgcagcgg taacccactc gacagcgtga cgcaaatggg ggcgcaggtt tctcacgggc
     6721 aactggaaac catcctcaac tacattgata tcggtaaaaa agagggcgca gacgtgctca
     6781 ccggcgggcg gcgcaagctg ctggaaggcg aactgaaaga cggctactac cttgaaccga
     6841 cgattctgtt tggtcagaac aatatgcggg tgttccagga agagattttt ggcccggtgc
     6901 tggcggtgac caccttcaaa acgatggaag aagcgctgga gctggcgaac gatacgcaat
     6961 atggcctggg cgcgggcgtc tggagccgca acggtaatct tgcctataag atggggcgcg
     7021 gcatacaggc agggcgcgtg tggaccaact gttatcacgc ttacccggca catgcggcgt
     7081 ttggtggcta caaacaatca ggcattggac gcgaaaccca caagatgatg cttgagcatt
     7141 accagcaaac caagtgcctg ctggtgagct actcggataa accgttgggg ctgttctgat
     7201 gtaagaagct ggtcgcatcg ggtattaatt gcctgatgcg acgcttacgc gtcttatcat
     7261 gcctacgggg acctgaccgt aggccggata aggcgtacac gccgcatccg gcataaacaa
     7321 agcgcacgtt gttacaattt acgttttaaa cctacgtgcg caataaccca gactacgcca
     7381 acagaagtgc gtttctgggg cgtgttttgc caggagttta atctcgcgtt tcgatggtga
     7441 gcgggcaaac cagaccgtaa atcatcattg gtattccgag gacaatcagc aataaacggt
     7501 agattattga aggtttaata cgcttaatct gcattgtgga tgttttacgg tttttaatca
     7561 taaattttgc caatagttcc attatttcaa tatgataagc ctcgacctgt taaaaagcca
     7621 ggcagcacac ttcaggaagt atataaaaaa caatatgtcg ttatataaat aagaaaattg
     7681 cactgcatca tggcatttca taatatgcgt taattgttct tttttatatt ctctgaggga
     7741 aatatgttcc tggactattt tgcactggga gtgcttattt ttgtatttct ggtgatattc
     7801 tatgggatta ttattttaca tgatattccc tacctgattg ctaaaaaacg taatcatcct
     7861 catgccgacg ccattcatgt tgctggttgg gtgagtcttt ttacgctgca tgttatctgg
     7921 ccatttttgt ggatttgggc cacgctttac cgcccggagc ggggatgggg aatgcaaaac
     7981 catgactcct ccgttgtgca actgcaacag cgcattgccg ggctggagaa acagctcgcc
     8041 gacatcaaat cctcttctgc cgagtaatac ttatggattt actgattatt ttgacctatg
     8101 tggctttcgc atgggcaatg tttaagatct tcaaaattcc tgtaaataaa tggaccattc
     8161 ccacagcggc cctggggggc atatttattg tcagcggttt aattctgtta atgaactata
     8221 accatccgta tacctttaaa gcgcaaaaag cggttatttc tattcctgtt gtcccgcagg
     8281 tgacaggcgt ggtgatcgaa gtgacggaca agaaaaatac gctaattaaa aaaggtgagg
     8341 tgctatttcg actggacccg acgcgttatc aggcgcgggt ggatcggctg atggcggata
     8401 tcgttaccgc agaacataaa cagcgggcgt tgggcgcaga gttagatgag atggcggcga
     8461 atactcagca ggcaaaggcc acgcgggata aattcgctaa agagtaccag cgttacgcac
     8521 gcggcagcca ggcgaaagta aacccgtttt cagaacgcga tattgatgtg gcacggcaaa
     8581 attatctggc gcaggaagcc tccgtaaagt catcggcggc ggaacaaaaa cagatccaga
     8641 gccagctgga tagcctggtg ttgggtgaac attctcaaat cgccagcctg aaagcgcagc
     8701 tcgcggaagc aaaatataac cttgagcaga cgatagtgcg tgcgccgagc gatggttatg
     8761 tgacccaagt gctgattcgt ccgggtacct atgccgcgtc gctgccgcta cgtccggtga
     8821 tggtgtttat acccgatcag aaacgacaaa tcgtggcgca gttccgtcag aactccttgc
     8881 tgcgcctggc tcccggcgat gatgcggaag tggtatttaa cgctctgcca ggcaaagtgt
     8941 tcagcggtaa gctggcagcc attagtccag ctgttcccgg cggagcttat cagtcgaccg
     9001 gcaccttaca gacgttaaac acagcgccgg gttcagatgg cgttatcgcg accattgaac
     9061 tgaatgagca cactgatttg agcgctttac cagacggtat ttacgcccag gtggcggtct
     9121 actctgatca tttcagccat gtctcggtga tgcgcaaagt gttattacgc atgaccagct
     9181 gggtgcatta cctttatctc gatcattaat cttcgctttc cgccgcttgt tcaggccgca
     9241 gttgctgcga ctgagcaagc tggtggcgaa tttcacgcag catttcatcc agcacatact
     9301 ggacccgcca gggctggtat tcccgtttac ggaagatagc caccagatcc agccaccatt
     9361 catacgggtg ctgaaagcag ggcaccagcg tgccgtcttg caggtcgctc tgcacacttt
     9421 tgtctggcgc aaacacaatc cccaggtgat ttcgtgccag ctccagcgca gattgcgtgt
     9481 tgtcgcaaac gtaatttccc ttcacgcgat aatcccgcac ctctttactc ccggcgacgt
     9541 tgaagcgcca gatattcgcg tcgtcgatca tcatcgagtc gatcaaaata caggagtgat
     9601 gttcgagttc gtcaggacgg ctaatgggat gtttctcaag atagcgctgg ctggcataag
     9661 cggttacggc atattgggta ataaaactgg caaccagcga ttcatctttt ggtggggcat
     9721 agctgattaa aacatcacaa tcatcaggaa attcgacgcc ttcataaaag gcattacgct
     9781 caagattgca ggtttttagc gataaggtga tatcactgat atctttaatt ttatcgatta
     9841 catgtttgga ta
//
LOCUS       DAZGBC010000172         9703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGBC010000172 DAZGBC010000000
VERSION     DAZGBC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.173"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(263..1960)
                     /gene="treA"
                     /locus_tag="ACHK1J_004208"
     CDS             complement(263..1960)
                     /gene="treA"
                     /locus_tag="ACHK1J_004208"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7913407.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPT
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHVPNGNRSYYLSRSQPPFFAMMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGIENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQREVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSELTTQPVKQKEAEPTP"
     gene            complement(2280..3698)
                     /gene="dhaM"
                     /locus_tag="ACHK1J_004209"
     CDS             complement(2280..3698)
                     /gene="dhaM"
                     /locus_tag="ACHK1J_004209"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO7913408.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSAVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNHQS"
     gene            complement(3709..4341)
                     /gene="dhaL"
                     /locus_tag="ACHK1J_004210"
     CDS             complement(3709..4341)
                     /gene="dhaL"
                     /locus_tag="ACHK1J_004210"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO7913409.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4352..5422)
                     /gene="dhaK"
                     /locus_tag="ACHK1J_004211"
     CDS             complement(4352..5422)
                     /gene="dhaK"
                     /locus_tag="ACHK1J_004211"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO7913410.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFVTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5650..7569
                     /gene="dhaR"
                     /locus_tag="ACHK1J_004212"
     CDS             5650..7569
                     /gene="dhaR"
                     /locus_tag="ACHK1J_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO7913411.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAVL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7669..8733)
                     /locus_tag="ACHK1J_004213"
                     /pseudo
     CDS             complement(7669..8733)
                     /locus_tag="ACHK1J_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            8980..>9703
                     /locus_tag="ACHK1J_004214"
     CDS             8980..>9703
                     /locus_tag="ACHK1J_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFO7913412.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIA"
BASE COUNT         2341 a         2400 c         2459 g         2503 t
ORIGIN      
        1 caaaatttgt ctaaagagac agaatgattt gcttgttgct taaataacac tttgttccac
       61 aagaaaattg tcagggagaa tggcggagtg gcgaaaaata taaatagagg tgtactgatt
      121 aacaaattga taaaataaaa ttaaaatctt ctctgatata agaaaataaa ggcatagacc
      181 gtagaatggg gggcatttcg tggcgaaaaa agagcaatgc caattttatg cacataatgg
      241 cattgctcga tatacagcaa agttaaggcg taggttccgc ctctttttgt tttaccggct
      301 gtgttgttaa ttcactgagt ggacgcgtcg ccggaacatt gtcacacggt tgctctttcg
      361 ggcagatcaa atccagcatt ttcagcgtca cgccattggt ccagccaaag ccatcctgta
      421 atggatattc gccaccgccg ccccccgttc cggtggtgct gacatcatat ttttccacca
      481 gctttttctc ccggtcatag gtgtgctgaa cattggtcag gaagtgccag ctaatgtcca
      541 tcgccacctc tctttgcccg tagttttgta atccttctgt cgcgacccac tgtaacggtg
      601 cccagccatt tggcgcatcc cattgttgcc cacttttcac cgacgtggtg ttcaggccgc
      661 cgggttgcag cagatgtgtt ttcgtcgccg tcgccatttt gttggcgcga tctttcgctg
      721 ccgcattgac gtacagcggg aacagggcgg ccgcggttaa ctgattgcgc actttatgac
      781 ttttcaggtc gtaatcggca taccagcctt gttgatcgtt ccacaggtat ttttcgatcc
      841 ctttttgacg ggcatttgcc agcgtttcgt actggtttgc catcgcgtta tctccggcag
      901 ctttgctggc gcgggcgagg attttttcca ttttaaacat caggctgttc agatcgaccg
      961 gtacgatgct ggtggtgcgt aaggtattta actgctgcgg gttgtccatc cagcgcgagc
     1021 tgaaatccca gccagacgca gcggcagagc gcaggtcgcg gtaaatttca gtggcaggtc
     1081 gattcggatt gcttttggcg gtggcaatat cttccaccca tgactctggt cgtggcgtat
     1141 cgcgatcatc ccagtagcgg ttgagaaggg taccatcctg aagttttaca acgcgttttt
     1201 cctgttgtcc ggcttgcagg ttttcaatac cgtccatcca gtaagcatat tctttttgca
     1261 tttgcggcag gtattgcttc aatgcagcat cgccttcatg ctgcgccagt aactctacca
     1321 tcatggcaaa gaagggcggc tgcgagcggc ttaaatagta actgcggttg ccgttgggaa
     1381 catgaccgta agtgtctatt tcatgagcaa aattggccac catatccgcg accttatccc
     1441 agtgaccgct ttcggcaagt cctaacatgg tgaagtaact gtcccagtaa tatacctcgc
     1501 gaaaacgtcc gcctggcacg acataaggtt ccggcagcgg taacagagaa tcccattttt
     1561 cggtgttttc ggtagaacgc gttaataccg gccaaagtcc gtcaatatgt tcgcgcagtg
     1621 actgtccctc tggcggaaca tatttctcgc cctctgtcgg cagggtgaaa ttgacgttaa
     1681 cgaaatggcg cagatcaaat ccgctctggt tttgctgcat ccgataatca gcaaggatca
     1741 tcagcggatc gctgttcggc acggcatcgg caaaggtttt ttggtccgga aaaagtttgg
     1801 cgttttgcac atcattaaac agcggcccta ataaaatatc aggcggctgt ggcgttaccg
     1861 gtgtttcttc tgcctgcacc gatagcgcag cgaaacacaa aaagatgcag gctggaatta
     1921 acgccatttt ttgcgggcga gaaggtgcgg gggatttcat caatcattct ccttaggcga
     1981 aaccgaataa aacggtattc aagtatcaga aaaccttagt tcaggatcgc actagctgca
     2041 tgatcgaaat ctcattctgc gaactgtcag gcattttaga aaagggaatt aaagggataa
     2101 ataacggcga aatagagtga tcaaataacg tctgtttatt agccagccag cggcgtttgg
     2161 ttcgcgatta gcgaagaaaa atcatcataa attggtgaaa aatataacag gtagttaaaa
     2221 aacattagtg ctgagtaaat tgccggatga catcagaacg atgccatccg aacggtggtt
     2281 taactctgat ggttgaaacg ttgcgtttta acgtccagcg ttagcgtttc ttctggttgt
     2341 atcgcataca gtttctcacc ctgctggcaa atccagccaa tccccagttc acgggcgatc
     2401 agggcgctgt gggataccgg acttccggcg ctaaggcaga taccttttac aaccgccgga
     2461 tccagttgca gtactgcgga gggataaatg ttctccgcca gtagaatagt tggcgagtta
     2521 aactgcggga gttcttcttt cgtttgggtc aggtggacca gggtgcgatg cagaagatcg
     2581 tccacatcaa tatagcgagc ttgtagatat tcatcatcca gttgctggta ttgctggcta
     2641 agttctttaa gaacttgctg ccaggcatat tctgccgtgc aatgttcgtg ctgaaggagt
     2701 tcgcttgccg ccgccagcag ttccggatca tctaacagtg tatggtgacc agaaaagatt
     2761 gcggcaatat cgtcaagccc gctggcttct gctttcgctg ttaacgtcat cagatctaat
     2821 aacgtgaagt caatggcctg gcgtaatcga tcttgttcct cttccacggt cagggttgat
     2881 ttggcctgta ccgtacataa aactggttga taataaaatg ctttacccga aacaggcgga
     2941 acgggacgca gagtaggtgg agcaacttcc tccgtttcac caaagttatc ttcagccagc
     3001 tgacggaaag cgatcagtgc ctcttcagct tctggccctt tcgcaatcag gcgcagcgta
     3061 tcgttatagc gaacttgtag taacgcaatc tggttaatac tctccggtgt gacgcatttg
     3121 ccgtttttct ccagcaacat atcggcatta aatgtcgata aggtataaac cagccgggag
     3181 gctggacgta catgcaggcc gttacggttc tttatgacca ccgccagaga acgggcttct
     3241 tcatcgtacg gaggacatgt gtcagagatt tcagtgtcgg aggacggtaa acccagttgt
     3301 tcacgtttgg cttccagcgc atgcatggcg tcaaagataa ctttgtcgat atccgccccc
     3361 gaggccgcgc tgaccgttgc tgccagtgta ccttcgacca acggtgcagc acacaaacgt
     3421 acttttgcag cgatctcggg agccagcaat tccagcgcag tttcagcact caataatgcg
     3481 ctacccatat ccatcatgac cagcacatgg tcggcatcgg caacagattc gatggcctcc
     3541 atcactttga cggcatcggt accaatggga ttttgtggat cgtcaattcc cgcggcaatg
     3601 gcgattttac aactatcact cattaacatc tgacgggcta attcaccgac accttctccc
     3661 agtcggctgc tatgtgaaac tatgaccagg tttaccatca ccaattcctt actcttttgc
     3721 ggctaacgcc aacatttgca tcataaacat caccgaggtt gcgccgggat cctggtgacc
     3781 aatactgcgt tcaccgagat aactggcgcg gcctttgcgg gcttgcatcg taatcgtact
     3841 ttgtgcagcg gattcggcga tgctactggc agcttcgagt gccgccggaa cagagagatt
     3901 ttgctcgctg gactgacgta acgattccac caccggcacc cacacatcac acatggtttt
     3961 atcgccaggt tcggctttcc cgcgactgat tacgccgtcc gcgccatcgc ggaacatctg
     4021 ataaagctct tccagcgtca ggctttgccg tgcctgggtc gcctgtgcgg cgcgtataaa
     4081 gaaggtaccg aatagcggac cactggcacc accgacgctg gaaagcagcg tcataccggt
     4141 attcttgaga atgaaaccga tatctttatc tgcgatagca gggagttttt ccaccacttt
     4201 gctaaagcct cggttcatat ttagcccgtg gtcagcgtcg ccaatctcgc gatccagtcc
     4261 ggtaagatac tcgctctcgg tgttgaaaat atcgccacaa cgagtgagcc agttaacaat
     4321 ttgagttctg ctcagtgaca ttgctttctc cttatttacc ccagttaagg gccggggtgt
     4381 ggaccggggc gtcccagagt gccagcgttt cgtcatcaac tttcagtaag gtgattgaga
     4441 aaccggtcat atccagtgag gtgcagtacg cgccaattaa attacgctcg atagtcaatc
     4501 ccgcttgctg gcaacgtgtg gtcaggcggt tatagacgcc gtacagctca gaaagtggag
     4561 ttgcgccaag attgttaacc agcgcaatca ccctgtcgcc agactggagc ggttgtttgg
     4621 tttgtggttc ttcctgccag ctgccttgtt gataatccca gaaacgcaaa gtgcgatggt
     4681 atgagccatt ttccagcagg gtgacgaaca tttcatcgac ggtttgatca agggaagaga
     4741 aggggcgacg gtcaataccc ggctcaccat gaatgccgac gccaaactcc atctcattgt
     4801 ccgccagggt aaaagaaggt ttgcccgcgg caggaacggt acaggcaccg agagcgatac
     4861 ctattgagtg gccttgatta ttcaacttac gccccagttc cgcacaggcg tccagtgagt
     4921 cgccacgctc cgccgctgcg cctacgagtt tttcaattaa tacggtgttg gcaacgccgc
     4981 gccgcccggc ggtataaaga ctgtctttta ctgcaacgtc gtcatcaatg accacagtgg
     5041 tcacttttac gccgctatcg tgcagtaact cggtcgctgt ttcaaagtta agaatatcgc
     5101 cggtgtaatt tttgataatc aacagtacac cttcgccgcc atcaacttgc atggcgcatt
     5161 caaagatttt atcgggcgtc ggtgaggtga aaatttcgcc cggacaggcc cccgaaagca
     5221 tcccctgacc gatataacca cagtgcatcg gctcgtgtcc gctgccgcca cccgacagca
     5281 gggcgacttt tcctgcaaca ggggcatcag ctcgggtgac atacaccgga tcctgatgca
     5341 gtgtcagcga tggatgcgct ttcgccagtc ctgccagttg ttcgtccagt acgtcttgca
     5401 catcattgat caattttttc attattttgc tccagcaatt acggtagggc atggatgatg
     5461 ttcaacgaca cggcgaccac tgactgccga tgaatccatt gtgcatcagg caagggaaaa
     5521 gaaaattagc ccaaattatg tttcatagtg aaacatatgc tttaatgaat gttccatatt
     5581 gaaactttta cgtgtattaa tacttaaaat tgagagccgg aacacctttt gtcataaggg
     5641 atgcgggata tgagtggcgc ttttaacaac gatggtcggg gcatatctcc cttaattgca
     5701 acctcctggg agcgatgcaa taagctgatg aaacgggaga catggaacgt accacatcag
     5761 gcccagggcg tgacatttgc ttctatttat cggcgtaaga aagcgatgct gacgctcggg
     5821 caggctgcgc tggaagatgc ctgggaatat atggcaccgc gagagtgtgc gctgtttatt
     5881 ctcgatgaaa ccgcctgcat tctcagccgt aatggcgatc cgcaaacctt gcagcagcta
     5941 agtgcacttg gattccatga cggcacgtat tgcgccgagg gaattattgg tacttgtgcg
     6001 ctatcgttag cggctatctc tggtcaggcc gtgaaaacga tggccgatca acatttcaaa
     6061 caggcactct ggaactgggc cttttgtgca acgccgttgt ttgacagcaa gggccgattg
     6121 acgggaacaa tagcgctggc gtgtccggtt gagcaaacta ccgcagctga tttgccgttg
     6181 acgttggcaa tcgcccgcga ggtcggtaat ttactgctga cggacagttt gctcgctgaa
     6241 actaaccgtc atttaaatca acttaatgcc ctgttagaaa gtatggatga tggcgtgatt
     6301 agctgggacg agcagggtaa tttgcaattt attaatgccc aggcggcgcg ggtcttgcgc
     6361 cttgacgcga cggcaagtca gggacgggca atcactgaac tcttaacgtt acccgccgta
     6421 ttgcaacaag caataaaaca ggcacatccg ctcaaacacg tagaagcaac ctttgaaagc
     6481 cagcaccagt ttattgatgc ggtgataacc cttaaaccga taatagaaac gcagggaacc
     6541 agctttattt tgttgctcca tcctgtggaa cagatgcggc agttaatgac cagtcaatta
     6601 ggaaaagtca gccatacctt cgctcatatg ccacaggacg atccgcaaac ccgccgcttg
     6661 attcattttg gtcgccaggc ggcgcgcagt agctttcctg tcctgctttg tggagaagaa
     6721 ggcgtgggca aggcactgct aagtcaggca attcataatg aaagcgagcg tgctgcaggt
     6781 ccttatatcg ccgtcaattg tgagttatat ggtgatgcgg tgctggcgga agaatttatt
     6841 ggcggcgatc gcacggacaa tgaaaatggc cgtctgagtc ggctggaact ggcgcacggc
     6901 ggcacgctgt ttcttgaaaa gattgaatat ctggcggtgg agttacagtc tgctttgctt
     6961 caggttatca agcagggtgt tatcacgcga ctggatgcgc ggcgtttaat accaattgat
     7021 gtcaaagtga ttgctacaac gaccgcggac ctcgcaatgc tggtggaaca aaatcgtttt
     7081 agtcgccagc tgtattacgc gctgcatgca tttgaaatta ccatcccgcc tctgcgtatg
     7141 cggcgtggca gcattccggc tctggtgaat aacaaattac gcagtcttga aaaacgcttc
     7201 tctacgcggc tgaaaattga tgacgatgcc ctcgctcgcc tggtttcctg tgcatggcct
     7261 ggcaacgatt ttgaacttta cagcgtcatc gagaatcttg ctctgagtag tgataatggg
     7321 cgcattcgcg tcagtgattt gccggaacat ctgtttaccg agcaggcaac agatgatgtc
     7381 agcgccacac gcctttccac cagtctgtca tttgcggaag ttgaaaaaga ggcaattatt
     7441 aacgcagccc aggtcacagg cggtcgcatt caggaaatgt cggctttact tggaatcggc
     7501 cgcactacgc tgtggcggaa aatgaagcaa catggcattg atgcagggca gtttaagcgc
     7561 cgggtatgaa agacagaaac gatttctgat acatcagagt gatctatatt tcattccggc
     7621 gcacgctaac atttttcagc atcgtttaag ggcttgtcta tcccgcactt aaaagctgaa
     7681 gcgatatcct ccgttgactt gcttctgatc aaagaggtta ccctgcgtgt aatccgcttc
     7741 gaggtagaat gtgtgctgtt tgttatactg tgcactgact cccacgccgt tattccagcc
     7801 attacctttg aagctgtact tctcccggga gttgttcaac agatattctg tatcccctga
     7861 aaactcgtgg atagcgccag tcttcacata gatattcagc tggcttttac ctgcggtgat
     7921 gtcatatccc agtatcatgc tggcacgccc cagcagcgat tcgtagtgat tcagatgtat
     7981 attgaggcca ttactcgcct tcatagccat ctcattctgg tggctgtatg tgagctgggt
     8041 ttgcggctct atatagaacc cataaccagt aggggtcagg ttgaacctct gcccggcttc
     8101 cagggagatg ctcaatccat tcgcagtgcc gttggcgtta acgccgttgt tctgactgtc
     8161 cagtacgtgg aaactatttt tctggcgcga tgcttttaca acgagatcgc tgtaaaaacc
     8221 gttgtgtgcc atgtaactgg cgtacattcc catgtagtct gaacgtgcgg taccgtcgcc
     8281 accgctatag tccggcgatg catgtgttga gccaatatac agaccgacat acaacggcat
     8341 tacatcagag agacgtttat ccccaccaaa ctggataccg ctgtaaccca tgtcaaagcc
     8401 gctcagtttg ccactggcga aggagtccag gcttccccca taactgcgaa gccagatatt
     8461 accgtcttta ctctgatttc gcaggtcacc cattcgttgc atcagcgtac ggttttcaac
     8521 atagtttaat aagtaaccga cattgagata attgccgcca gcatctgcgg tcgtggtggg
     8581 tttaggtgtg ggagtggggt taggcgttgg atcggggttg actatgggag gctgagcggg
     8641 agccggcgtt ggttcaggat ttggagtcgg ctccggaatt gtcccggaag cataaagctc
     8701 ccagttagtg ccatttttac gcacatcgta caattaatac agcgtttagt aattagtaaa
     8761 gacatttcaa tggattaccg ttaaatcatt ttaaaatcag taagttgtat cgagtttgta
     8821 tgctttactg tcattaactt actgtatgtt gatccagtgt atttaacctt gataaactca
     8881 gtccagcaac acaaaaccgc aacacattgc attttgtccc gtagaaaaga cttgtatgtg
     8941 tgagcttgtt ttctgcgcct acgcagataa ggattgagaa tgccgcgcac tgtaacacat
     9001 aatccggata gccccaataa tgacgatgtt ttagccgcat ctgaaaaatg ggacgcctgt
     9061 aaacccccct ataccagcgc acacatgaaa atctgtgttg ctgccgctaa aatcatcctc
     9121 gctgcttccg gcgtggctcg ccgttccaaa tacgaaaaag agaactatct ccgtatcgat
     9181 ttcagcaaag ccggtaaggt tacattttat gccgagtttc caaaaaagat gggcctcaaa
     9241 ggtaaaaagc tcggcgagtg gccggagctc gctatccagc tggcgcgcga aaaagcgcta
     9301 ggtatggctg acggtggcct gcgggcagag tccgtacatg cagcgctgga aatgtaccgg
     9361 gatgacctca aagccaaagt cgcccggcag aagctgagcc cggacagttt cacaacctac
     9421 ggggtgcgta tcgaccggat taaagccacg ttcggcgagc gcgaggtgtt cagcgacgta
     9481 acatacaatc ggctggtgga agtgctggac gagtggatcg ccactcgctc gaacaataac
     9541 gccctggagt tgtttgccga gctccgtcgg ttctggaagt tctgcgcacc tactctttgc
     9601 aatggccgca atgttgccgc cagtctgcca gatgattatg tttcctcccg cgtacagaaa
     9661 cccaccccca cacggctttt taccgatatt gaatcaatcg ccc
//
LOCUS       DAZGBC010000173         9652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGBC010000173 DAZGBC010000000
VERSION     DAZGBC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.174"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..1220
                     /gene="ulaG"
                     /locus_tag="ACHK1J_004215"
     CDS             156..1220
                     /gene="ulaG"
                     /locus_tag="ACHK1J_004215"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO7913413.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1328..2083
                     /gene="ulaR"
                     /locus_tag="ACHK1J_004216"
     CDS             1328..2083
                     /gene="ulaR"
                     /locus_tag="ACHK1J_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO7913414.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2080..2829)
                     /gene="yjfP"
                     /locus_tag="ACHK1J_004217"
     CDS             complement(2080..2829)
                     /gene="yjfP"
                     /locus_tag="ACHK1J_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7913415.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3011..3340
                     /gene="bsmA"
                     /locus_tag="ACHK1J_004218"
     CDS             3011..3340
                     /gene="bsmA"
                     /locus_tag="ACHK1J_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO7913416.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3489..3764
                     /gene="yjfN"
                     /locus_tag="ACHK1J_004219"
     CDS             3489..3764
                     /gene="yjfN"
                     /locus_tag="ACHK1J_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO7913417.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3881..5506)
                     /gene="aidB"
                     /locus_tag="ACHK1J_004220"
     CDS             complement(3881..5506)
                     /gene="aidB"
                     /locus_tag="ACHK1J_004220"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO7913418.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5590..6753)
                     /gene="yjfC"
                     /locus_tag="ACHK1J_004221"
     CDS             complement(5590..6753)
                     /gene="yjfC"
                     /locus_tag="ACHK1J_004221"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7913419.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6756..7394)
                     /gene="yjfM"
                     /locus_tag="ACHK1J_004222"
     CDS             complement(6756..7394)
                     /gene="yjfM"
                     /locus_tag="ACHK1J_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO7913420.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7404..7802)
                     /gene="yjfL"
                     /locus_tag="ACHK1J_004223"
     CDS             complement(7404..7802)
                     /gene="yjfL"
                     /locus_tag="ACHK1J_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO7913421.1"
                     /translation="MHILDSLLAFSAYFFIGVVMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7820..8479)
                     /gene="yjfK"
                     /locus_tag="ACHK1J_004224"
     CDS             complement(7820..8479)
                     /gene="yjfK"
                     /locus_tag="ACHK1J_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO7913422.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8530..9228)
                     /gene="yjfJ"
                     /locus_tag="ACHK1J_004225"
     CDS             complement(8530..9228)
                     /gene="yjfJ"
                     /locus_tag="ACHK1J_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO7913423.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9247..9648)
                     /gene="yjfI"
                     /locus_tag="ACHK1J_004226"
     CDS             complement(9247..9648)
                     /gene="yjfI"
                     /locus_tag="ACHK1J_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO7913424.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSDNALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEIASLVDNALDLAEITEEYSH"
BASE COUNT         2512 a         2546 c         2289 g         2305 t
ORIGIN      
        1 acattccatt aaatctttcc atactccaaa ttcacaacaa atcacccggc aaaaagtcaa
       61 aaaatggtca ttgccttcct gaaaaaatag tgtcaggatt aatcaaaacc aatcatatgt
      121 tgattcgaat tgaactatca tgaaggtaaa tggcgatgag taaagtgaaa agtatcaccc
      181 gtgaatcctg gatcctgagc actttcccgg agtggggtag ctggttgaat gaagaaattg
      241 aacaagagca ggtagctcct ggtacatttg cgatgtggtg gcttggctgc accgggatct
      301 ggctgaaatc ggaaggtggc accaacgttt gcgttgattt ctggtgcggc acaggcaaac
      361 aaagtcacgg taacccgtta atgaaacagg gtcaccagat gcagcgtatg gctggcgtga
      421 aaaaactgca gccgaacctg cgcaccaccc cgtttgttct tgatccattt gctattcgcc
      481 agatcgacgc cgtcctggcg actcacgatc acaacgatca tatcgacgtt aacgtcgctg
      541 ctgccgtgat gcagaactgt gctgatgacg taccgtttat cggaccgaaa acctgtgtgg
      601 atttatggat cggctggggc gtgccgaaag aacgttgcat cgtggttaaa ccgggcgacg
      661 tggtgaaagt caaagacatt gaaattcatg cgcttgatgc tttcgaccgt actgcactga
      721 tcaccctgcc tgccgatcaa aaagcggctg gcgtactgcc agatggcatg gacgatcgcg
      781 cggtgaacta cctgttcaaa acgcctggcg gctccctgta tcacagcggc gactcccact
      841 actctaacta ttatgcgaag cacggtaacg aacatcagat cgacgtggcg ttaggatcgt
      901 acggcgaaaa cccgcgcggt atcaccgaca aaatgaccag cgccgatatg ctgcgtatgg
      961 gcgaagcgct gaatgcgaaa gtagtgatcc cgttccacca cgatatctgg tcaaacttcc
     1021 aggccgatcc gcaagagatc cgcgtgctgt gggagatgaa aaaagatcgc ctgaaatatg
     1081 gctttaagcc gtttatctgg caggtgggcg gcaaatttac ctggccgctg gataaagaca
     1141 acttcgagta ccactatccg cgcggtttcg atgattgctt cactattgaa ccggatctgc
     1201 cgttcaagtc attcctgtaa tctgtcgttt ataccggata gcgtggagaa gttcgcgcta
     1261 tccggcgtag tatttcctgg ctatttcaaa tatcatctaa aaaaatcaaa ttttatcgga
     1321 atagctcatg actgaagcac aaagacatca aatcctcctg gaaatgctcg cacaattggg
     1381 ctttgtgacc gttgagaaag tcgttgagcg tctgggaatt tcgcctgcca ctgcgcgacg
     1441 cgatatcaat aaacttgacg aaagcggcaa actgaaaaaa gtgcgcaatg gcgcagaagc
     1501 tattacccaa cagcgcccgc gctggacgcc gatgaatctg catcaggcgc agaatcacga
     1561 tgaaaaagta cgtattgcta aagcggcctc gcagctggtt aatccgggcg aaagcgtagt
     1621 catcaactgc ggctccaccg cgtttctgct tgggcgggaa atgtgtggca agccagtgca
     1681 aatcatcact aattatctac cgctggcaaa ttacctgatc gatcaagaac atgacagcgt
     1741 gatcattatg ggcggacagt acaacaaaag tcagtccatc actttaagcc cgcagggcag
     1801 cgaaaacagt ctctatgccg ggcactggat gtttaccagc ggaaaagggc tgaccgcaga
     1861 agggttgtat aaaaccgata tgctgacagc aatggcagag cagaagatgc tgagcgtggt
     1921 agggaaactg gtggtactgg ttgatagcag taagattggc gaacgcgcgg gaatgctttt
     1981 tagccgtgcc gatcaaatcg atatgcttat caccggcaaa aatgctaacc cggaaatcct
     2041 gcaacaactg gaagcgcaag gggtcagcat tctgcgtgtt taaagatgct ggcggaaaaa
     2101 tgtcacggca gcatctaacg cctcaggcgt aatgcggtga cgcacgcctg gctgccatga
     2161 acaggttagc agtttatccc gtcccgtctc gcttaaggcc tgctgcaaac gtagtgattc
     2221 atcggcaggc acaacgtcat cgtcgaggcc atgccacagc agtagaggtc tgtccccaag
     2281 ttgttccagg tggtttgtcg cttcccactc tgccagcggc gcgacaatgt tattgaattc
     2341 attctgctgt gctgccgttt caggtatcag cggtggaaac agtgaacggg cgagcgatgt
     2401 aaaatagccc gatcccatca tgctggcggt acatttcacc gtggggtggc gagcggtaat
     2461 tcccagtgcc gtcatcgcgc ccatcgacgc gccaccgact gccagacggt catcaagcag
     2521 ccagttttct tcggctattg ccgcacgtaa agtagtgaat tcctgcatac tttgtagcaa
     2581 gatttgccag aattgattta accgccgcgc tgcgtcgcca ctaaaacgac taccgtgatc
     2641 gggcgcatcc ggcatgatca cccgcaaacc agcctgcgcc agcgcaacgg caaaatagct
     2701 atacaccaga ctggacgaag taaagccgtg ataaaaaatc acgcacggta acggggtatc
     2761 tttttgtccg acaggataag catgaagaac gggaatatct gccagctcgc gtgattctat
     2821 ttcaatcatc tgtctctcct ttttgtctgg tatgacatga agaaatcctt agcacaagtt
     2881 tctgctggcg gtattagttc ataatgttga gatgtgggtt acgctttcgt cacgttttca
     2941 ttcgaaaatc gcgtaccagg taacaattcg ggaacattcc cccaaagtta aattaataag
     3001 gcactacact atggttagca ggaaacgtaa tagcgttatt taccggtttg ccagtttatt
     3061 attggtgttg atgttaagtg cctgtagcgc actgcaaggt acgccacagc cagcaccgcc
     3121 agtaacggac catccgcaag agattcgccg cgaccagacg caagggttac agcgaatagg
     3181 tagcgtaagc acaatggttc ggggttctcc ggatgacgca ttagcagaaa tcagagcaaa
     3241 agctgtcgct gcaaaagctg attattacgt tgtcgtcatg gtggacgaaa ccatcgtgac
     3301 aggacagtgg tattcacaag ccattttgta ccgtaaataa atcagacaac ttacatagtc
     3361 agctttacat tgctttgcgt ccctatgcgt ttacctgtgc acaataaatt accggctgga
     3421 agcctgggcg tttattaccg cgatggaatg ccctgcaaca tgtggggaaa cgaaaaatgg
     3481 agctgacgat gaaacaatta cttgcctcac cctcgctgca attagtgact tatcctgcga
     3541 gcgccacggc acagtctgca gaattcgcca gtgctgactg cgtgacgggc ctgaatgaaa
     3601 taggtcagat ctcagttagc aatatttcag gggatccgca ggacgtggaa cgtatcgtgg
     3661 cgttaaaggc cgatgaacaa ggtgcgtcat ggtaccgcat tattaccatg tacgaagacc
     3721 agcagcctga taactggcgc gtacaggcga tactgtatgc atgagtgtca tctacagcaa
     3781 atgaacaatg ccggatgcgg cgcgagcgcc ttgtccggcc tacaattttc acatatttca
     3841 ttgagttaat cgaaaccagc gtcgcatcag tcgtatacgc ttacacacac actccccccg
     3901 tcgcccgcag caataaatca ttctggatct gctctgacaa ccgtacgccg ccgcgcgtat
     3961 ctaacatcac ctgacaccac gcctgcgcca ttggcggaga ggcatatttc aacatttgcg
     4021 caccgcagcc cagcaggaat agctgatgag taatctctcg ccccagttct tcagctggct
     4081 tacgcagctg ctgctgtaaa cgacgaaccg cgcgatcaaa atagcgatcc tgccctttca
     4141 cttccacaaa tgcttccgac aataagtcgt atacgcccgc ttgcttattg agaacgcgca
     4201 gcacatccag gcacataata ttgccggaac cttcccaaat actgtttacc ggcatctccc
     4261 ggtaaagccg cggcagctcg ctctcctcgc aataaccaat gccgcccagc acctccatcg
     4321 cttcggccac aaacggcata cctcgtttgc agatcacaaa ttttgccgca ggcgtaaata
     4381 aacgcgccca cagggcttct ttggcatcgg ctcgccggtc ccacgctcgc gcaagacgaa
     4441 acagcaacgc cgtttgccct tcaagctgaa gtgccatgcg acttaagaca tgacgcataa
     4501 ggggctgttg gatcaatgga ttaccaaaaa catggcgttg atgcgcatga taaatcgcca
     4561 gcgagaacgc acggcgcatc atggcatggc tacccagggc gcaatcaaaa cgcgtcatcc
     4621 cccccatttt caggatcaga cgaattcctt ccccttcctg ccccaataac cagccaatgg
     4681 catcctgaaa ctccacttca caactggcgt tagagcgatt acccagctta tctttcagcc
     4741 gctcgaggcg aatcgcgttg cgttgcccgt caggcaaaaa gcgcggcaca aaaaagcagg
     4801 acaaaccgcc cgcggtctgc gccagcacca gatgcgcatc gctttgcgga accgagaaaa
     4861 accatttatg ccccaccagc cgataagagc catcctccag acgctctgcg cgggtggtgt
     4921 tgctcatgac atcggaaccg ccctgctttt ccgtcattcc catgccaatc aacaaaccgc
     4981 gtttttgccc acctggcaat aagtgagaat cgtagcgatc gctcaacagc ggcgtggtcc
     5041 agtcctgaaa cggcgtgggt aacatctgta acaacaatgg cgtggcggca aaggtcatcg
     5101 ttattggaca taacgacccc gcctcaacct gtgcgtgcaa cataaaacgc gccgcgcgcg
     5161 ccacaaatgc gccggagcga gcgtcttctt cccaggcaag attgtgcacc cgattggtac
     5221 atagcgcctg catcagcagg tgccaggcgg ggtgaaaacg cacatcgtcc agacggcgtc
     5281 cttgcgcatc gtagcgcaat aattcaggcg gattcacatt cgccagccgc cccagttcaa
     5341 gggattcagc cgttcctaac tgctgaccaa tactggctag aaaatcgcta tcccagccag
     5401 caccttcacg cgttaccgct tcgcaaagcg cgccatcaga caggtataag ttgctgttat
     5461 ttaatggtat aggttgatta aaaacggtgt gagtttgcca gtgcactgtg tctccctcca
     5521 tcaatggcag tcaccattag tatggtcact gccatggatt catgacagaa agagattgct
     5581 aaaacattct tatccagcaa tgtaatgcgg aacgaaacgt gaggtgtctt tggtgatcag
     5641 tgtgttatct tcacgaatcc ccattccgca cgcttcatca tcgacaatcc agctaccgat
     5701 gagtgtgtag ctatcgccaa accgcggcag aggttgaaac gcctggtaga tcatcggttc
     5761 atcggcgtaa tcaccatcag cgtggtcaac gacgttattc tggccgtcga aaatggtgac
     5821 atttccgcct tcgcgcgagt agatcggttt acgcacatag ctttcgccag cggcaatctg
     5881 cggtttttca ccctcaaacc aggatgcaag aaggttagga tgaccaggga agaagcgcca
     5941 aagcagcggc attagccctt tattactcaa gatacttttc cataacggct ccacccattg
     6001 ctcgcgacgc ttgcgcagca gcggaccgtt atcgtcacgc atcatccact ccagcggata
     6061 cagcttaaat gcacgctgga tgacattatc atcaagatcg gtcagtacgc cgccgacgcc
     6121 caaaccgaga tcttcaatgt agataaaccg cgactcctgc cctgcctgct gggcgcagtc
     6181 ctgcaagtac agcacggtag tcctgtcttc atcggtgtcc tgacagcagc agaaataaaa
     6241 cggctcccga ctgtaaagct cactaaagcg cgaaatcagg cgttcctgaa tggcattgta
     6301 ctgatcggca tcacgcggaa taatgccgct gcgccgggca tcctctagcc acaaccactg
     6361 gaaataagcc gactcataca acgaggtcgg cgtatcggcg ttgtactcca gcagcttcac
     6421 cggcgcattg ccacaccagg caaaatccat ccggccatac agcgaaggat cgcgtgcgcg
     6481 ccagctttca gcgatcacat cccagtacaa cggagggatc gccagttgcg tcaggatctc
     6541 ttcgtcttta accgcgcgat ccaccacctc aaggcacatc tgatgcagtt ccgcagtcgg
     6601 tttttcgatc tgctcttcaa tctggcgcag agtaaaacgg taagcccgac tctcgtccca
     6661 atagatttca ttgtcgatga tatgaaagtc gaaaccgttg tcagcggcga tttggtccag
     6721 atcccgtcgc acaggaacgt tgtgtctcag catgattagc ctccccaatg cccacgggcg
     6781 ctggaagaac gaccatagcc gccgcgagaa acggttgacg cttttttggt ggtaaagccg
     6841 cctgaagagt aagactcttt tttgccagaa ccggagcgcc aggagtaatc accagaagtg
     6901 ttgcgccaga cggggcggga agcaaatgag gatccgccgc tgttataaac aaactgctca
     6961 tcgcgatctt tacgaataac ccggcttaac aaaaatccag aaacgaccgg gatccaactc
     7021 tgttcaacgt tgtcgtagta gcaattttcg tacttagact gacaattctg ctgagtcata
     7081 ttcttcggaa catcggcata aaatgctgct ttggcgttat tccagccacg cgcgcaaatg
     7141 tctgcattat taccgtcatc aatacaatcc tgcactgtcg cgtaaaacgt tccgtcgccg
     7201 tcgttatcga catcgccact atcgccacaa cctttcaata caaaaaaagc ggcaccgccc
     7261 attagcgcca acgttaagta tttttgtgcg tagcgattgc gacgcggttc aaaaggatta
     7321 ttcggtctac caatgcgact gatcgctccg tgaccaattt tactgttatt tcttgatttg
     7381 cgttttctgg ccatgatcgt ccattaccat gtcatacaag ctgcgttaaa aataccgcca
     7441 gccagcgcgg cggttcccat aaacattcct gctgcggtat tgtgattaat aattttttcg
     7501 cttaatgcgg gcatataaag tctgacacca gcaaaaacga gtaactgaat caccagtgca
     7561 atcccgcccc aggcgaaata gtctggaata ctcaccgcat tgattgccgc actggataag
     7621 gggatcacgt aacccaacaa tgtaccgctg aacgccagtg acgctgcggt attattgttt
     7681 ttgattaatt gccattcatt gtgcggtgta attttggagt agataaacag aaaaataatc
     7741 accatcacca cgccaataaa aaaataggca ctaaaggcaa gaagtgaatc cagtatatgc
     7801 attgtaaacg tccttgtaat taaccaataa tatgaagtga tgtcagcggg atatcgacac
     7861 ccaacgcgcg cgaaaataac cactctggct cgccgagatc gttaaaagat tcctcaccat
     7921 ttagcagcaa atattcgtaa gtatcttcgg tcacttgccg ctggtagccc atggtaaaat
     7981 tatggacttc ccatttagca tgattttgat tttctacttt ttccagcatg tagacgggtt
     8041 cgatatttcc cggttcttcg ctgttaaaaa atctctgcca gcgtttttcc tgccagttta
     8101 aggtcattgc ccccatcgtt ttggcgttga tcgcctcgcg ccagtgactc tctttactga
     8161 taccgtagct ctcttcatag acaaagagct tgatatcatc aatgtcatca atatcttcgc
     8221 cgccggtggt attgatttgc agaaactcat cgcctgaagt gtagtagcgg aaaatctgac
     8281 taccgccgcc cagatcgatg tggctgacag cggctaccgt aaattcctca cccggcagcg
     8341 caatcagcaa ttcatcttcc agcaaacgaa acgctaacgt gtcgagcgta aagccactat
     8401 tgagatgaag tcccagcgga ccgcgagcga tagcaggctt attatccttg ccaaacagac
     8461 gctggaaaaa accagacata ttttatcctt aaaatagttg ccagccttgt agtgaggctg
     8521 gcaaaaaaat tactcgccct gttggcgttg cagtcttgct aatacatcct gggcgctact
     8581 tttattgcta ccgccaattc cggcttccgc cagcttttcg tcaaggtcgc gaccgtctgc
     8641 gactttctcc aactgtgcgg cagcatccag gcgagcctga cgttctgcct gacgcgtttg
     8701 caggcgtttt aaggattctg ccgctgtcga aacgctggaa gatgcgccaa cggtagaggt
     8761 tgttacagcc tgttgtgcac gctgcatggc ttcagtggct ttaacgactt ccatttgctg
     8821 ctcaaactga gcgatacgct gcgctgtcgc tgtaactgct ttttccacgc catcacggga
     8881 gacttccaga ttcgacaaca cttgctcttc agcggtaatg agattctcaa ggcgcgcgat
     8941 ttcttcagca acttcgttaa tcaacgacgg attaacgttc ttgcttaacg cttccagcgc
     9001 acgggcttcc agactggctt tgcgctcacg taaatctttc agcttatcgt gacttaattt
     9061 cacccgcgcc agcagatcga cgcgagattt tccggcttta tcgagttcag ctttagcgtc
     9121 acgaatatgc tgttccagca tgcgcacgcc ctgggtttct tcaatggact cttccgcctg
     9181 ggagataaac gatttcccca gcgtgaataa actttttaaa attcccataa atacccgtcc
     9241 ttgtaattag tgtgaatatt cttctgtaat ttcagccaga tccaatgcgt tatctaccag
     9301 cgaggctatc tcaagcagga tatcttcaag agaagattta agcgataacg ccccgaaaac
     9361 aatgtaatac tcttcctgtt gcacgctgga gattcctacc gatgacaacg gcatcatctt
     9421 ctgatttctt aataagaagg tattaaattc atctggattg ctgatactac ttaccggaca
     9481 aataaaggtt tcgataatca tttggcggga agtaaaaaga atattgattt gcaaatcgcc
     9541 ataatcattc attttaataa ttaatgcgtt atcgctattt gttacatcaa tattttgttc
     9601 aggtacagtt tgcagcgccg tcgccagcgc caacggattc catgtcatat at
//
LOCUS       DAZGBC010000174         9616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGBC010000174 DAZGBC010000000
VERSION     DAZGBC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.175"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1891
                     /gene="yjiT"
                     /locus_tag="ACHK1J_004227"
     CDS             <1..1891
                     /gene="yjiT"
                     /locus_tag="ACHK1J_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048814575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YjiT"
                     /protein_id="HFO7913425.1"
                     /translation="NGKRASWVCHPDETFIGVPKVISTLPDIQSIDVTRYLSGISIEQ
                     CHIQEMLGAQYLSIRNSNNETLLRRKIGILPADFSIEIKGGMHANEGTIVITTQHPCV
                     ATLKDKTLEATRKRTEGRTEIQLKAEGVPPAFITLQVLPNLAADTIDIELPFPAKGCL
                     ALDVNGRPLDKNITLHDLLGSRAFLFSRNGEPTKYTLQLHLRSISGLQAWHEWSYTAL
                     SERPVELNLYSLREHIENLISLEAGIDQVVEMRITGAGVVMAWQIRRYKYSLRYDYEK
                     ELLLSQSVNHRAGQIPSPVIMLLSEPERKSIPLVSRMSEGVPVGEYELSSIVNKNGPW
                     LVVPKPGEEMAFRPCFIRGESSLPVEESNIRSLQKATQLFNPQAEVNAITLVLGQMAN
                     DPAHSGWQFMRSLYDQFGYLPLATFEVWRALVQHPQALAMSLFKFEMSAEYLSRIENE
                     FPILWEFFPIFEIKAASERFKLFLSQKGAPEETQKLLVTNMFQRLGLVFPTYADEIEK
                     WLSNGYLPPSIPESCVHGWYQELLREHSEARWPEYGCKRLYKWMMSQKNPVIDINPDA
                     NHRYSVAWLPVFAAAVASGNASFESVFDRKPGAVFFLRQVRDFDSRWFKAIFQCSLLR
                     YVAKK"
     gene            1904..8248
                     /locus_tag="ACHK1J_004228"
     CDS             1904..8248
                     /locus_tag="ACHK1J_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO7913426.1"
                     /translation="MTTRYFSSLIEQSLSRSTEATLSIMGVTNPKLREHLAQQMGADC
                     GKPGSFLASPVFQQMFGWKESNHTMRSLTEGNALLSKAVVDSLDDQSNGRYRFGADWK
                     PFTHQLASWKALLEKKHSVVVTSGTGSGKTECFMVPVLEDLYRELHENGNNPLVGVRA
                     LFLYPLNALINSQRERLDAWTRGFGTGIRYCLYNGNTENLHASVKSEQAKRPNEVLSR
                     EKMREEPAPILVTNGTMLEYMMVRQVDAPIIQQSKAQKSLRWIVLDEAHTYVGSQAAE
                     LALQLRRVMTAFGVTPDDVRFVATSATIAGSDAEKQLKKFLSELSGIAQERIDVLDGS
                     RVIPELEPCKNVSVPLDEIEQIPDLDLKDKNDNKIKGISPERFYALTHSPEARYIREM
                     LVSQPNPMKLDEMTQRLNALTKQHYSQQEVLRWIDVCSGTQPNAKDPAFLKVRAHIFQ
                     RNTQGIWACVDKNCRLKHGTPLEKGWPFGYVYVNQRQNCDCGSPVYEVAFCNDCNEPH
                     LLAQDKKGKLVQWENKGGDEFSLQDEVPVEHDATEEKVEKENSFQPPLIIAAGETSEA
                     GYTLQRLDRQTRRIGVINNDSIPLIINDVEQVCSASGCGYRGMSGKQPFRRALLGGPF
                     YVTNIVPTVLEYCQDFTSDEGKEGIGPDSLPGRGRRLITFTDSRQGTARMAVRMQQEA
                     ERSLLRGSVVEILSWHQRTQTSTAPNANADLEKLAARAKQAREQAEEYRSWGMPDQAK
                     LSQAQAEQLEQAYQAATGGKAATTLVSRTWTEMVNELKDKADIRGPVLKYNHYLQPEV
                     FNKNGGPLKLSEMLLFREFMRRPKRTNSLETQGLVQVGYQGLEKIHKSPLHWQEKGLT
                     LDDWRDFLKVTLDLYVRESNFTQLDDELKNWIGSRFSSKFVRNPESKDPEDNQNRRWP
                     QIRNGNVSHRLAKLLMLGAGFKTVNTATIDIINTWLKEAWAQLTGPLAVLKPDGNRFY
                     LPKEHMTFSLITDAWICPVTNKILDTAFKGLTPYLPTHISFEHLTQAQYDTFVAQKVT
                     MPEIWKLDRSQEDYAEGLAKARDWVNNDPLIAQLRSENVWTDINDRVVEGGFYYRTAE
                     HSAQQSSERLQSYEKMFKNGQLNVLNCSTTMEMGVDIGGITAVVMNNVPPHPANYLQR
                     AGRAGRSKESRAISYTLCKGNPHDQQVFANPLWPFETMIPAPMVAMNSARLVQRHVNA
                     LLLSDFLCNVIGETDKEKTSLDSLWFFGEDDGQSKCERFKVWLERPVLDIDTALERLV
                     KGTALHGARAEHLRDKTINAITFLQQRWLSVYRDLVTQERESQPQTPYRKRIELEKKR
                     HCGEYLLRDLAARTFLPGYGFPTDVVTFDNFTMEDYIREKSQKSRDKKDREDNVSRYK
                     GLPSRNLGVAIREYAPGAEIILDGRVFRSAGVSLHWHNINADTNEAQRLDCAWRCHKC
                     GTIGYEEGMSSSGMLFCSNSACGEKITMDNRRQVLQPAGFVTDAHAPVTNNIETMKFV
                     PVVPAWVFVKAEPVPLPNPLMGYMASGADGHVFQQSLGEGGHGYALCLSCGRAESMLN
                     ENDAPKSMEAHYPPRPGKADRDSQNHRLICPGSTALMKNVTLGALARTDVFEMVLRKP
                     QNGEYLPDNTEEGRIVAMTLAVALRQALAGVLGISAAELGYSVRPVRLEDGQSVLAVQ
                     LYDVISGGAGFASSAPVHIEAILQGMVKQLGCRHCDTACSECLLDSQTRHDHDLLDRK
                     VALAWLGDDFTYYIGLPDEETFSLPDARYCPGAIGDTIRRAINEGAEKLTLWMTGAPN
                     EWDLYARQFRAAIQSYRLKDNVEVDLVIPAGVDDPDLLHELSQFTALGVRLCHVEQEL
                     QLPIVAQVTFADRVMTLASRSQQATIPGPEWHLNDELVVRSLGYQTVELNEFILPAKA
                     ANAVERVKDIQIHKQLNGPLSQFGQRFWDVLFNDHEEAQSLMKNTRITGVHYTDRYLQ
                     NPVALALLGSILKPLKTKLTDGAEVALDTLFKDKDRPGNRPFHDWMSIADFQDFADQW
                     FAAALGRPIELTVFDSPRDIPHHRKLTVTFEDGQVLKIRFDQGMGYWRINFASQWHYF
                     DFRDDVSFQLVKMAQACKEGNVANSEESWATDVLVEVTAS"
     gene            complement(8291..8632)
                     /gene="symE"
                     /locus_tag="ACHK1J_004229"
     CDS             complement(8291..8632)
                     /gene="symE"
                     /locus_tag="ACHK1J_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO7913427.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPVAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVIAGKQKVA"
     gene            complement(8860..>9616)
                     /locus_tag="ACHK1J_004230"
     CDS             complement(8860..>9616)
                     /locus_tag="ACHK1J_004230"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010671752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7913428.1"
                     /translation="FQLPADCWEWVCFEQICETDNNALKAGPFGSALKKSDCVKSGHK
                     VYGQEQVISGQESLETYRITEDKYQQLKSCATKPGDILISLVGTIGKTLILSNLAETG
                     IINPRLVKIALHSEIQRQYVKLYLDGPLAYDFFKGFSHGGTMDILNLGILKELPIPLP
                     SLEEQREIVRRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENP
                     ELISGENSAAALLEKIKAERAASGGKKASRKKS"
BASE COUNT         2479 a         2246 c         2503 g         2388 t
ORIGIN      
        1 aaatggaaag cgtgcaagct gggtttgtca tccagatgag acatttattg gtgtaccaaa
       61 agtcatttct acactaccag atattcaaag cattgatgta acgcgttacc tgagcggaat
      121 ttcaattgag caatgtcata tacaagagat gctaggggct caatatcttt ctatccgtaa
      181 tagtaataat gaaacgctct tgcgtcgcaa aatcggtatc ttgcctgctg atttcagtat
      241 tgagataaaa ggtggaatgc acgcaaatga aggcactatc gtaatcacca cacagcatcc
      301 ttgtgtagca acacttaaag ataaaacatt ggaggctaca cgtaagcgta ctgaggggcg
      361 aacagagata caactcaaag cggaaggggt acctccggca tttattacat tacaagttct
      421 gcctaatttg gcggctgata ccatcgacat tgagttacct ttcccagcta aaggctgcct
      481 tgctcttgat gtaaacggac gtcctctgga taaaaacatt acgctgcatg atctgctggg
      541 ttccagagca ttcctgttct ccagaaatgg tgagccgaca aaatatacat tgcagttgca
      601 tttgcgctcc attagtggat tacaggcatg gcacgaatgg agttatacgg cattgagcga
      661 gcgtcctgtt gaattgaatc tctatagtct gcgcgaacat attgagaacc tgatttcact
      721 tgaggcaggt attgatcagg ttgttgagat gcgtattacg ggggcgggtg tggtcatggc
      781 ctggcaaatt cgtcgctata aatacagcct gcgctatgat tatgaaaaag aactattact
      841 cagccagtca gtgaaccatc gtgctgggca gattccttcc cccgttatca tgctgttaag
      901 tgaacccgag aggaaatcca ttcccttagt atctagaatg agcgagggag tgcctgtcgg
      961 tgaatatgag ttgagctcaa tcgttaacaa aaatggacca tggttggttg ttcctaaacc
     1021 gggtgaagag atggcttttc gtccctgctt cattcgtggg gaatcttcgt taccggtaga
     1081 agaaagcaac atccgctcat tgcagaaagc gacccagctt tttaatccac aggcagaagt
     1141 taatgccatc accctggtgc tcggacagat ggctaacgat cctgcccatt ccggctggca
     1201 atttatgcgt agcctgtacg atcaatttgg ttatttaccg ctggcaacct ttgaagtatg
     1261 gcgtgcactg gtgcagcatc cgcaggcatt ggcaatgtcg ctcttcaaat ttgaaatgtc
     1321 agcagagtac ctgagtcgta ttgagaatga gttccctatt ttatgggagt ttttccccat
     1381 ctttgagatc aaagctgcat ctgagcgttt taaattgttc ctgagccaaa aaggtgcacc
     1441 ggaggaaact cagaagttac tggttactaa tatgtttcaa cggctggggc tcgtgttccc
     1501 gacatatgcc gatgaaattg agaaatggtt aagtaatgga tatctccccc cgtctattcc
     1561 tgaatcttgt gtccacgggt ggtatcagga gctcttacgt gagcacagtg aggctcgctg
     1621 gcctgaatat ggctgcaagc gtctgtataa atggatgatg tcacaaaaaa atccagtgat
     1681 tgatattaat cctgacgcaa atcaccgcta ttccgtggcc tggttacccg tcttcgccgc
     1741 agcggtggca agcggaaatg cttcttttga atctgtattt gaccgtaaac ccggtgccgt
     1801 atttttcctg aggcaagtca gggatttcga ttcccgctgg tttaaggcca tcttccagtg
     1861 cagcctgttg cgttatgttg ctaaaaaata aaaaggaata aggatgacta cacgctattt
     1921 ttcgtcactt attgaacaat cgttgtctcg ttcaactgaa gccacgctca gtatcatggg
     1981 cgttacaaac ccgaagttgc gtgaacatct cgcgcagcaa atgggggctg attgtgggaa
     2041 accgggctca ttccttgctt ctccggtatt ccagcaaatg tttggctgga aggagtcaaa
     2101 ccacacgatg cgcagcctga cggaaggcaa cgcattgttg agcaaagcag tggttgattc
     2161 actggatgat cagagtaatg gccggtaccg gtttggtgct gactggaagc cttttacgca
     2221 ccagttagcc agttggaaag ctttgctgga aaagaaacat tcggtggtgg taaccagtgg
     2281 tacgggttca ggtaaaacgg aatgttttat ggttccggtg ctggaagatc tttaccggga
     2341 actgcatgaa aatggcaaca atccgttggt cggtgtccga gccctttttc tctatccatt
     2401 gaacgccctg attaattcac aacgcgagcg tctggatgcc tggacgcgtg gttttggtac
     2461 cgggatccgc tactgccttt acaacggcaa caccgaaaac ctacacgctt cggtaaaaag
     2521 cgaacaggca aaaaggccga atgaggtgct ctcgcgcgaa aaaatgcgtg aagaacctgc
     2581 gccgattctg gtgaccaacg gcacgatgct ggaatatatg atggttcgcc aggtcgacgc
     2641 cccgattatc cagcaatcca aagcacaaaa atcactacgc tggattgtac tggatgaagc
     2701 gcatacctat gtcgggtcgc aggccgctga actggcgctc cagctgcgtc gtgtgatgac
     2761 agcgtttggc gtgacgccag atgacgtgcg ctttgtggcg acctctgcga ccattgccgg
     2821 cagcgacgct gaaaagcagc tgaaaaaatt cctctctgaa ttatccggca ttgcgcagga
     2881 gcgcatcgat gttctggacg gaagtcgggt gattcctgaa ctggaaccat gcaaaaacgt
     2941 ttctgttccg ttagacgaaa tagagcaaat acctgatctg gatctaaaag ataaaaatga
     3001 taataaaatt aaagggataa gtcctgagcg cttttatgcg cttacgcact cccccgaagc
     3061 gcgttacata cgtgaaatgt tggttagcca acccaatcca atgaaactgg atgagatgac
     3121 gcagcggtta aatgcgctga caaaacagca ttacagccag caggaagtac tgcgttggat
     3181 cgatgtctgt tcagggactc aacctaatgc aaaggatccc gcatttctta aagtcagggc
     3241 gcatatcttc cagcgtaata cccagggaat atgggcttgt gtggataaaa attgcagatt
     3301 aaagcacggt acaccgctcg aaaaaggctg gccctttggc tatgtgtatg tgaaccagcg
     3361 acaaaattgc gactgtggaa gccctgtcta cgaagtggcg ttctgtaatg attgtaatga
     3421 gcctcatctt ctggcgcagg acaaaaaggg caaacttgtc caatgggaaa ataaaggtgg
     3481 cgatgagttc tccttgcagg atgaagtacc tgttgaacat gacgctacag aagaaaaagt
     3541 cgaaaaagag aacagtttcc agcctccgtt gattattgcc gcaggagaga ccagcgaggc
     3601 cggttatacc ctacaacgcc tcgaccgcca gacgcgccgt attggcgtta ttaacaatga
     3661 cagcattccg ctgattatta atgatgttga acaggtttgt agcgccagtg gctgtggcta
     3721 cagaggcatg agtgggaaac agcccttccg gcgtgcacta ttaggtgggc cattttacgt
     3781 tactaatatc gtgcccaccg ttcttgagta ttgtcaggac tttaccagtg atgaaggcaa
     3841 agagggcatc ggaccagatt cgttgccagg gcgaggacgt cgactcatta cctttacgga
     3901 cagtcgacaa ggtacagctc gaatggcggt gcgtatgcag caagaagcag aacgcagtct
     3961 cttacgcgga agcgtagtcg aaattctgag ctggcatcaa cggacgcaaa cgtccacggc
     4021 gccgaatgcc aatgccgatc tggaaaaatt agcggccagg gcgaagcaag cccgtgagca
     4081 ggcagaagaa tatcgaagct ggggaatgcc agaccaggcg aaattgtcac aagcgcaggc
     4141 tgaacagctt gaacaggctt atcaggctgc aaccggtgga aaagcagcaa ctaccctggt
     4201 atcccgcacc tggacagaaa tggttaacga gcttaaagat aaagccgata tccgtggccc
     4261 ggtactgaaa tataaccact atttgcagcc tgaagtgttt aataaaaacg gcgggccact
     4321 aaagctttcg gaaatgttgt tgttccggga attcatgcgt cgccctaaac ggactaacag
     4381 cctggaaaca caggggctgg ttcaggttgg ttaccagggg ctggagaaaa tacataagag
     4441 ccccttgcac tggcaggaaa aaggattaac gctggatgac tggcgcgatt ttctcaaggt
     4501 tacgttggat ctttatgttc gcgaaagcaa cttcacacag ctggatgatg agctgaaaaa
     4561 ctggattggt agccgttttt catcaaaatt cgtccgtaac ccggaatcaa aagatcctga
     4621 agataatcag aacagacgct ggcctcaaat tcgtaatggc aacgtatccc atcgtttagc
     4681 gaagttactg atgctggggg ctggattcaa aaccgtcaat acggcgacta ttgatattat
     4741 caatacatgg ctgaaagaag catgggccca acttaccgga ccacttgcag tactgaaacc
     4801 cgatggcaac cgtttttatt taccgaaaga gcatatgact ttttctttaa tcacggatgc
     4861 ctggatttgc ccggtaacca ataaaatcct ggatacggct tttaaaggct taacccctta
     4921 tctgcccacc catatttcgt tcgagcatct tacccaggcg caatatgaca cttttgtcgc
     4981 gcagaaagtc accatgccgg aaatctggaa gctggatcgt tcacaggaag actacgcaga
     5041 gggtctggca aaagcacgag actgggtcaa caatgatccg ctgatcgcac aattgcggtc
     5101 ggaaaacgtc tggacagaca tcaacgatcg tgtcgtggaa ggcggtttct actatcgcac
     5161 tgccgagcac tctgcccagc agtcgtcaga acgcctgcaa agctatgaaa agatgttcaa
     5221 gaacgggcaa ctaaatgttc tcaactgttc caccaccatg gaaatggggg ttgatattgg
     5281 gggcattact gcggtggtaa tgaacaacgt tccgccacat ccggccaact atttacagcg
     5341 cgccgggcga gccgggcgta gtaaagaatc acgcgcgatc tcttataccc tatgcaaagg
     5401 taacccacac gaccagcagg tttttgccaa cccactctgg ccatttgaaa ccatgatccc
     5461 ggcacccatg gtcgcgatga actccgcccg actggtgcaa cgtcacgtca atgcattatt
     5521 actgtctgac tttttatgta acgttattgg cgaaaccgat aaagagaaaa ccagcctcga
     5581 tagcctctgg tttttcggtg aagatgacgg gcagtcaaaa tgcgaacgct ttaaagtctg
     5641 gctggaacgc ccggttcttg atatcgacac ggcgctggaa cggctggtga aaggtacggc
     5701 gttgcatggc gcaagagccg aacatctgcg ggataaaaca atcaatgcga tcaccttcct
     5761 gcaacagcgg tggctctccg tttatcgcga tctcgtgacc caggagcgtg agtcgcagcc
     5821 gcaaacgcct tatcgtaagc gcatagagct ggagaagaaa cggcactgtg gcgagtatct
     5881 gctgcgcgat ctggctgcca gaaccttcct gccgggatac ggtttcccga cagatgtagt
     5941 gacgtttgat aacttcacga tggaagatta tatccgcgaa aaatcgcaga aaagtcgtga
     6001 taaaaaggac cgtgaagata acgtctcccg ttataaagga ttaccttccc gtaacctcgg
     6061 tgttgccatt cgtgaatatg caccgggagc agagatcatt cttgatggcc gtgtcttccg
     6121 ctctgccggt gtctcactgc actggcacaa cattaatgct gataccaatg aagcccagcg
     6181 tctggattgc gcctggcgct gtcacaaatg cgggaccatt ggctatgaag aggggatgag
     6241 cagttccggg atgctctttt gctccaacag cgcctgcggc gaaaaaataa ccatggataa
     6301 tcgccgtcag gtgttgcagc ctgccgggtt tgtcaccgat gcacacgctc cggtaacgaa
     6361 caatattgaa accatgaaat tcgtcccggt tgtccccgcc tgggtctttg ttaaagcaga
     6421 gcccgtaccg ttgcctaacc cgctaatggg ttatatggct tctggggctg atgggcatgt
     6481 tttccagcaa agtctggggg aaggtggaca cggttatgca ttgtgtctga gctgtggccg
     6541 tgcggagtcg atgctgaatg aaaatgatgc tccgaaaagt atggaagctc actatcctcc
     6601 gcgtccgggt aaagcggacc gcgacagcca gaatcatcgt ctgatttgcc ccggttcgac
     6661 agcactcatg aagaacgtca ccctgggcgc actggcgcgc accgatgtct ttgaaatggt
     6721 tctgcgcaaa ccacaaaatg gcgagtatct gccagataac acagaggagg ggcggattgt
     6781 cgccatgact ctggcggttg ctctgcgtca ggcgctggca ggcgttctgg gtatctccgc
     6841 cgcagaactc ggctattctg tacgtccggt cagactggag gatgggcaat cggtattagc
     6901 cgttcagctt tatgatgtga tcagtggtgg tgcaggtttt gcctccagcg caccggtaca
     6961 cattgaagct atattgcagg gaatggtgaa acagttaggc tgccgccatt gtgatactgc
     7021 atgtagtgaa tgcctgcttg attcccagac tcgccacgat cacgatttgc tggatcgcaa
     7081 agttgcgctg gcctggcttg gcgatgactt cacgtattac atcggtttac cggatgaaga
     7141 gacattctcg ctgccggatg cccgctactg ccctggcgct attggcgata cgattcgccg
     7201 ggcgattaac gaaggtgctg aaaagcttac cctatggatg accggcgcgc ccaatgagtg
     7261 ggatttatac gcccgtcagt tccgcgcagc tattcagagc tatcggctaa aagacaacgt
     7321 cgaggtcgat cttgttatcc ctgcgggagt ggatgacccg gatctattgc atgaactttc
     7381 gcaatttact gcgctgggtg tgcggttgtg ccatgttgag caggagctgc aattaccgat
     7441 tgttgcccag gtcacctttg ccgatcgcgt gatgacgctg gcctctcgca gccagcaggc
     7501 aacgattccg gggccggagt ggcacctgaa tgacgaactg gtggtgcgta gtctcggtta
     7561 tcaaacggta gaactgaacg agtttattct gcccgctaaa gccgccaacg ctgtcgagcg
     7621 ggtcaaagac attcagatcc acaaacagtt gaatggacca ctttcgcagt ttgggcaacg
     7681 cttctgggac gtgctgttta acgaccatga agaagcgcag tcactgatga aaaacacgcg
     7741 aattaccggc gttcactata ccgatcgcta tctgcaaaac ccggttgcgc tggcgttgtt
     7801 gggatcaatt ctgaaaccgc taaaaacaaa gctgaccgat ggcgctgaag tggcgctgga
     7861 tacgctgttt aaagataaag atcgccccgg taaccggcct ttccatgact ggatgagcat
     7921 agcggatttt caggatttcg ctgatcagtg gtttgctgct gcgctgggca ggccgataga
     7981 actgacggtg ttcgattcgc cgcgcgatat ccctcaccat cgtaaactga cggtgacttt
     8041 tgaggatggt caggtattga agatccgctt cgatcagggg atgggctact ggcgcatcaa
     8101 ctttgcatcg caatggcatt actttgattt ccgcgatgac gtttctttcc agttagtcaa
     8161 aatggctcag gcctgcaagg aagggaatgt ggccaacagc gaagagagct gggcaacgga
     8221 tgtgctggtg gaggtgaccg cctcctgatg atgtgccgct cccgacgtcg tgtcgggagc
     8281 gtttttcaat tcaggcaact ttctgtttac ccgcaattac cccaataaaa tcctgcacct
     8341 gcttttgttt acgcgccgac agcttgcaca cctggcgcag cgactgcatc agttcgctct
     8401 cttcggcgac gggtggttgg gcggtgagga caatacagcc ttccattact ttgacatcta
     8461 ccgccgtgcc ggtggcaaaa ccggcggctt ccagccactg acctttcagg gtaatggcgg
     8521 gaatacggct gtaatccggg tagcggctcg cataactcac ggttaattga cggttatttg
     8581 ccggggagac ttctgcttcg aacggttgtg caatagaatg cgtgtcagtc atgattacta
     8641 ttctccataa atagtgattg tgatgagcgg tgcgggtgtg ttggcgcaca tccgcaccgc
     8701 gctaaatacc tggatatatc atcagtaaat acaggaaaag tccagctaaa aatagaataa
     8761 aatgggcaat ttctggaatg aataaaatat atttgcgtga gttatgattg gcgtgaaata
     8821 ataaaaagcg caccggaaag gtgcgccaga aaataatgat caggattttt tacgcgaggc
     8881 ttttttaccc ccgctggctg cgcgttcagc tttgattttc tccagcaacg cggcggcgct
     8941 gttttcaccg ctgattaatt ccgggttttc ggcccgccac tgggcagtga gctcaccacg
     9001 gaatgctttt gccaggatgg attgcgtcag attgttgacg cgggctaagg cgttgttgac
     9061 ctgtttttct atggtgtcgg cgtaggcgaa gagttgctcg acgcggcgaa cgatttcgcg
     9121 ttgttcctcc agcgatggga gaggaatagg taactctttt aatataccaa gatttaggat
     9181 atccatagtc ccgccatgac tgaatccttt aaaaaagtca tatgctaagg ggccatctaa
     9241 atacagcttt acatattggc gttgaatttc actatgtaat gctattttta ctaggcgtgg
     9301 atttattata cctgtctctg caagattaga tagtattaat gtttttccga tagtaccaac
     9361 taaactaata agaatatctc ctggtttggt ggcgcaggat ttcaattgtt gatatttatc
     9421 ttctgtaatt ctataagttt caagtgattc ctgtcctgag ataacttgct cttgaccata
     9481 aactttatga ccagatttaa cacagtcaga ttttttgagt gccgaaccaa atggcccagc
     9541 ttttaacgca ttgttatcag tctcacaaat ctgttcaaaa catacccact cccaacaatc
     9601 ggcaggcaat tgaaat
//
LOCUS       DAZGBC010000175         9524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGBC010000175 DAZGBC010000000
VERSION     DAZGBC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.176"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..107
                     /locus_tag="ACHK1J_004231"
     CDS             <1..107
                     /locus_tag="ACHK1J_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7913429.1"
                     /translation="ISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(91..729)
                     /gene="ytfB"
                     /locus_tag="ACHK1J_004232"
     CDS             complement(91..729)
                     /gene="ytfB"
                     /locus_tag="ACHK1J_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO7913430.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            948..1568
                     /gene="fklB"
                     /locus_tag="ACHK1J_004233"
     CDS             948..1568
                     /gene="fklB"
                     /locus_tag="ACHK1J_004233"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7913431.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1877..3289
                     /gene="cycA"
                     /locus_tag="ACHK1J_004234"
                     /gene_synonym="dagA"
     CDS             1877..3289
                     /gene="cycA"
                     /locus_tag="ACHK1J_004234"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO7913432.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3334..3996)
                     /gene="ytfE"
                     /locus_tag="ACHK1J_004235"
     CDS             complement(3334..3996)
                     /gene="ytfE"
                     /locus_tag="ACHK1J_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO7913433.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4104..5069)
                     /gene="ytfF"
                     /locus_tag="ACHK1J_004236"
     CDS             complement(4104..5069)
                     /gene="ytfF"
                     /locus_tag="ACHK1J_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7913434.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGPLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5177..6037)
                     /gene="qorB"
                     /locus_tag="ACHK1J_004237"
     CDS             complement(5177..6037)
                     /gene="qorB"
                     /locus_tag="ACHK1J_004237"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7913435.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHRFNVNN"
     gene            6126..6506
                     /gene="ytfH"
                     /locus_tag="ACHK1J_004238"
     CDS             6126..6506
                     /gene="ytfH"
                     /locus_tag="ACHK1J_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO7913436.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6624..8567)
                     /gene="cpdB"
                     /locus_tag="ACHK1J_004239"
     CDS             complement(6624..8567)
                     /gene="cpdB"
                     /locus_tag="ACHK1J_004239"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO7913437.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8757..9497
                     /gene="cysQ"
                     /locus_tag="ACHK1J_004240"
     CDS             8757..9497
                     /gene="cysQ"
                     /locus_tag="ACHK1J_004240"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO7913438.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2166 a         2438 c         2512 g         2408 t
ORIGIN      
        1 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
       61 tccttagctg gcttctggtt gacccttcat ctaccgcgcg cgaataaaac tgccatccgg
      121 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ctgtcaccac
      181 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggttt
      241 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      301 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      361 tccgctatct ggctggaatg gttgtgtttg ggtagtttgc ggctgctctt ctggctggcc
      421 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      481 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgtgactgaa tatctaactg
      541 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      601 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      661 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      721 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
      781 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
      841 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
      901 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
      961 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1021 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1081 cgctggaagg caaacatccg gctgttccgg ttgatgtggt gcatcgcgca ctgcgtgaaa
     1141 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1201 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1261 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1321 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1381 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1441 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgtg
     1501 gcgcaggcgc gtccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1561 tcctctaagc agcgcattct gttcccctcg agccagaggg gagcaggcat tcagcaataa
     1621 ccccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1681 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1741 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     1801 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     1861 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     1921 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     1981 tgccattggt acggggttgt ttatggggtc tggcaaaacg attagccttg ccgggccgtc
     2041 gatcattttc gtttatatga tcattggttt tatgctcttt ttcgtgatgc gggcaatggg
     2101 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2161 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2221 tatggcagac gtggtggcga tcacggctta tgctcagttc tggttccccg atctctccga
     2281 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg cttaacctcg ccaccgtgaa
     2341 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2401 gattgtcgtc ggcctggtca tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2461 gtcattcgcg catttgtgga atgacggcgg ctggttcccg aaaggtttaa gtggcttctt
     2521 tgccggattc cagatagcgg ttttcgcttt cgtggggatt gagctggtag gtacaacagc
     2581 tgcggaaacc aaagatccag agaaatcact gccacgcgcg attaactcca ttccgatccg
     2641 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2701 ggtagtcccg gagaaaagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgctgc
     2761 cgcaagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     2821 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     2881 cgctaaactt tctaagcgcg cagtaccagc gaaagggctg acgttctcgt gtatctgtct
     2941 gctcggcggc gtggtgatgc tgtatgtgaa tcccagcgtg attggcgcgt tcacgatgat
     3001 cacgaccgtt tccgcgattc tgtttatgtt cgtctggacg attatccttt gctcgtacct
     3061 tgtgtatcgc aaacagcgtc ctcatctaca tgagaagtcg atctacaaga tgccgctcgg
     3121 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3181 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cgctggggct
     3241 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3301 tgctggatgg cgcaatgcca tccagctttt agatcactca cccgccagcg cgcgtgggaa
     3361 cagcacatta ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatgcc
     3421 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtatt
     3481 gtgtttgatc acttccagca gttcacccgc ttcatcgtgc tcgctttcca ttacgctgat
     3541 tggccccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3601 tttcatcatg tggctggaaa gctcttcatg cagcatggtc aggtattttg tcagcccttt
     3661 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca gaatcagctc
     3721 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcga tgatttctgc
     3781 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagctcagc
     3841 ttcaatgacc tcaacatcca gttctttacg tgccgccgca cgcgccagcg tttgcttacc
     3901 gccgcagcag taatccatat catatttacg aaacagagcc gaagcgcgag gaatagagag
     3961 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataag
     4021 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4081 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4141 ggttttgctc tgaccgcgat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4201 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4261 aaaacaatca gcggcccgag aatcacggtc ggtaatcgct ggctggcgac gttccagcag
     4321 agtgcgccga cccatgagca aagcacggct atcgcaatca tcagactaat aaacaccagc
     4381 ggacgggggc caaagggtag ggagaagtcc ggcgtttgcg tattcagcca gtaacaagcg
     4441 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4501 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4561 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4621 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4681 atcagtgccg gggcgagttt tccccacgcg agtttgccgt cgcgctggct ataaagcaga
     4741 ttggcaaaga caggaatgac caccggcagg gggccgataa tcatcgtgga aacaggcgcg
     4801 ccagtacgtt gaatggcact ggcaaggcag aagtaataaa tgaggttgcc catcatagtg
     4861 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     4921 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     4981 gccgggtatt ccggcacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5041 aacagggcgt acagcacgcc gctaatcatt actggcatcc attgatctgt cagaagaaag
     5101 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5161 atgccacttt aattaactag ttattaacat taaaacgatg gcttacgctt tcggctaacg
     5221 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gtcatcaaat agaccgcctt
     5281 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5341 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5401 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5461 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5521 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5581 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5641 tgtaaacgat gccagaatca gccaacattt tctccgtctc aatgtgctca tcggcgaggc
     5701 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     5761 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     5821 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     5881 agtcagcctg acgcacggta atgccttgtg ctgccagggc ctgggctttt gccggattac
     5941 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaagattca ataacatagt
     6001 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6061 tgagtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6121 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6181 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6241 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatg ggcggggtga
     6301 gtgaaaagat gcttgcgcag tcgttacagg cgctagagca ggatggtttt cttaaccgta
     6361 tcgcgtatcc ggtggtgccg ccgcatgtgg aatatagcct cacgccgctg ggcgagcagg
     6421 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttag
     6481 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6541 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6601 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6661 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6721 gctttatctg acggggaagt ttcgaaacgg atatccagtt tcttatcgcc agctatcggt
     6781 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     6841 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     6901 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     6961 atagcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7021 gcattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7081 ttcacaccat caataacatc aaagttatag gtgcggaaac cgtcccagtt gatgagtgac
     7141 tgtggtttcg tgctgttggg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7201 tctttcacct ctttaccgct ggctttcacc acaatcagcg tattgggata gagataaaga
     7261 tcggcggcat tacggaaagt cagctgacct ttttccacct ctacatagct tgccgggtca
     7321 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7381 tccggatcgc cctgaatgta atgctcgaca tacgcttttt gcgcgttgtt caccacctgc
     7441 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7501 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7561 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgtgcttcc
     7621 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7681 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7741 gcgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatgg
     7801 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     7861 atcactttat acggatcggc agatagcccg gaatgcgcca gaacgacaac gacatcggca
     7921 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     7981 actttcccgg ataaattagc tttatcccag cccatgattt gtggcggcac gacgccaata
     8041 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt gtctttaatt
     8101 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8161 gggaatttcg ctcctgccag cgcatttttc aggtaatcca gaccgtagtt aaactcgtgg
     8221 ttgccaagcg ttccgacggt atagtccagc gtatttaatg ccttatagac cgggtgaata
     8281 tcgcctgctt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaag
     8341 tcgccgttat caaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8401 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8461 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8521 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8581 ttatcatcgg gaatacgaaa gaaaagggag aataaacgcc ttacttatag aacagtgaag
     8641 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8701 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     8761 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     8821 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     8881 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     8941 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9001 tagacccact ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9061 ttgcgctcat tgatcatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9121 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9181 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9241 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9301 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9361 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9421 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9481 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttc
//
LOCUS       DAZGBC010000176         9341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGBC010000176 DAZGBC010000000
VERSION     DAZGBC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.177"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(275..3799)
                     /gene="nifJ"
                     /locus_tag="ACHK1J_004241"
     CDS             complement(275..3799)
                     /gene="nifJ"
                     /locus_tag="ACHK1J_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO7913439.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKVRF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            4073..4339
                     /gene="ydbJ"
                     /locus_tag="ACHK1J_004242"
     CDS             4073..4339
                     /gene="ydbJ"
                     /locus_tag="ACHK1J_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO7913440.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(4336..4758)
                     /gene="hslJ"
                     /locus_tag="ACHK1J_004243"
     CDS             complement(4336..4758)
                     /gene="hslJ"
                     /locus_tag="ACHK1J_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO7913441.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4869..5858)
                     /gene="ldhA"
                     /locus_tag="ACHK1J_004244"
     CDS             complement(4869..5858)
                     /gene="ldhA"
                     /locus_tag="ACHK1J_004244"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7913442.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            6066..8705
                     /gene="ydbH"
                     /locus_tag="ACHK1J_004245"
     CDS             6066..8705
                     /gene="ydbH"
                     /locus_tag="ACHK1J_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO7913443.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVGAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQVSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            8702..8887
                     /gene="ynbE"
                     /locus_tag="ACHK1J_004246"
     CDS             8702..8887
                     /gene="ynbE"
                     /locus_tag="ACHK1J_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO7913444.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            8895..9218
                     /locus_tag="ACHK1J_004247"
     CDS             8895..9218
                     /locus_tag="ACHK1J_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO7913445.1"
                     /translation="MKRTLLIAVWAIGLMSDGAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
BASE COUNT         2285 a         2287 c         2427 g         2342 t
ORIGIN      
        1 tatcatttgt tacgccttaa gtaaaacctt aatcaaataa ttcctctgaa atgataataa
       61 gtctggtgaa tgtatcgaag ataatacata caaaataaaa attatacttt ttattttcta
      121 tacgttattc tgcgcgggtt atatgccttt attgtcacag attttatttt ctgttgggcc
      181 attgcattgc cactgatttt ccaacatata aaaagacaag cccgaacagt cgtccgggct
      241 ttttttagaa ttggataatc cttatccaga gcatttaatc ggtgttgctt ttttccgctt
      301 ttccggccat ttgtgccagg aagtcatagc gtttttgcaa atcagctgca gcatctttcc
      361 ataactgttc tgccacttct ggctgctgcg aattcagacg acggaaacgt tgctcgtgaa
      421 gtaacgtttc ttccagtgct tctgacggcg ggcgtgaatc cagcgccagc ggcagtttgc
      481 cttcatcggc acgacgcgga tcgaagcgat atagcggcca gaagccggta gctgtgagtt
      541 ggcgcatctg gtcatggctg agtgccagat cgtaaccatg ctcttcacat gggctataag
      601 cgatgatcag cgatggtccc ggatacgctt ccgcttcctg aatcgctttc accgtctggt
      661 tcagttgcgc gccgagagaa atctgcgcca cataaacatg accgtacatc atcatactga
      721 cgccaagatc tttacgcgct ttacgtttgc cgtgctcgcc gaacttagtt actgcaccca
      781 gcggtgtcgc tttcgacgcc tgaccaccgg tgttggaata gcattgcgta tccagcacca
      841 gaatgttgac gttttccgtc aaactcaata catgatccag accgccaaag ccgatatcgt
      901 aagcccagcc gtcgccacca atcagccaga ttgatttttc taccagtgca tctgcgtcac
      961 gcagcagttc atgtgcttcg gcaacatcgt tgagttgctg gcgtaaagct gcgacctgtt
     1021 ctcgacgaac ctctggcgta gcgtctgatt tcaacgccgt cagtaattcc gccgggattt
     1081 tatcggcaaa ttgatccagc agacgcagca cgcggacacg gtgttgatcg accgtcaggc
     1141 ggaaaccaag gccaaattcg gcattatctt caaatagaga gttcgcccat gccggcccac
     1201 gaccgttggc atcggtggta tacggtgtag agggcaggtt accgccataa attgaagaac
     1261 agccggtggc gttagcgatc aacatccggt cgccatagag ctgagtcagc aatttaatat
     1321 acggcgtctc gccacaaccg gagcaagcac cggaatattc aaacagcggt gtaatcagct
     1381 gcgatgtacg aatatcaata cgttccagtt tgctacggtc gatttctggc aggttgagga
     1441 agaaatcgta attgattttc tcttcttcga catgttcaag gcgcgacatc atattgatgg
     1501 ctttaatctc tggattctga cggtctttcg ccgggcaaac ttcgacgcac aggttacaac
     1561 cggtgcaatc ttccggagcc acctgcaaga catatttctg cccgcgcata tcacgcgatt
     1621 tcacatccag cgaatgcagg ctggcagggg cgttttccat cgcttcaggc ggtaccactt
     1681 ttgcgcgaat agctgagtgt gggcaagcgg caacacagtg gttacattgg gtacagagtt
     1741 cctctttcca gatggggatc tcttcggcga tattgcgttt ttcccagcgt gtagtgccca
     1801 tcggccaggt gccgtctggc ggcagcgccg aaacggggag ggcatcacca agcccggcga
     1861 gcatcgccgc ggttacggtt ttcacgaaat caggggcggc atcggaaacc actggcggtc
     1921 gattggcgct gtgcggatta accggctgca acggaacttc ttctacggat tcacgcgcca
     1981 gcgccagagc ctgccagttg cgttccacca gatcctgacc tttgctactg taacttttgg
     2041 caatcgcacc ctgcaattct gcgagggcgc tatcgccagg cagaatttgc gtcagatgga
     2101 aaaaagccat ctgcatgacg gtattaatac gggccgccag gccacattcg cgggcgattt
     2161 tcgccgcgtt aatcacatag aagcgcgctt ttttctgatt tagcacggcc tgaacttcct
     2221 gcggcaagcg cgaccacact tcatctgcgc tgtacggcgt gttgagcagg aaaatgccgc
     2281 caggttttaa acgctcagcc atctgatatt tatcgataaa ctgcaactgg tggcagccaa
     2341 caaaatcagc ctgggaaatg agataagcgg aacgaatcgg ctgttcgctc actcgaaggt
     2401 gagaaaccgt caggccgccc gcctttttgg agtcgtaaac aaaatagccc tgtgcgtacc
     2461 acggcgtgga attaccgata atcttgatat tgtttttggt cgcggaaacg ctgccatcac
     2521 taccaagccc ataaaacaag gcttccagtt tcgccgagtt tggcagggtg ttttccggca
     2581 acggcagtga cagattggtc acatcatcgt aaataccaac cgtaaagcgc actttcggtt
     2641 tagccgcgtt gagctcggca aataccgcca gtacacagtc cgggccaaat tctttggatg
     2701 aaagaccata gcgcccacca atgacacggg gcagagtttc gcgctcgcca ttattaaagg
     2761 cttctgccag tgcggtcatt acatccagat agagcggttc tgcctgggca ccgggttctt
     2821 tggttctgtc cagtaccgcc acgctgcgta cggatcccgg cagagcttgc agcaagtgtt
     2881 tggcggagaa gggacggtac agacgaactt tcagcacgcc aactttttcg ccacgggtta
     2941 gcaattcatc aaccacttct tcacaggtac caatggcaga gcccatcagg ataatcaccc
     3001 gttccgcttg cggatgcccg taatattcaa acggctgata ctgacgacct gtcgcggcag
     3061 cgaaatcatt catcgcctgt tcaacatggt catagaccgc gttgtaccat gggttggtgg
     3121 cttcgcgaga ctggaaataa gtgtcaggat tggcggacgt accgcggatc accggatgtt
     3181 ccgggttgag tgcccgggcg cgatgagcat caatttcggc ctgcggcatg agatcaagaa
     3241 tcgtgtcatc ggccagcggg acaattttat tgatttcgtg ggacgtgcgg aaaccatcaa
     3301 agaaatgaat aaatggcacg cggcttttca gcgtcgcgat gtgcgaaatg agagcaaagt
     3361 cttgcgcttc ctggacgttt gccgcacaca acatcgcgca acccgtctgg cgcaccgcca
     3421 tgacgtcgga atgatcgcca aaaatagaga gtgcatgtgt ggcaacggta cgtgccgcta
     3481 catgcaggac aaacggtgtg agttcgcctg ccagtttgta cagcgtcggg atcatcagca
     3541 gcaaaccctg cgatgacgta aacgatgttg aaagggcacc cgtctgcaaa gcgccatgca
     3601 cggtagcgat agcacccgct tccgactgca tttcaaccac gcgtggtgtg tctccccaaa
     3661 cgttcttcaa gccgtttccg gcccaggcat cagcctgttc tgccatcgtg gaactggggg
     3721 taatagggta gatggcgata acttcactgg tgcgaaacgc caccgaagca accgcgccat
     3781 taccgtcaat agtaatcata tgacaccctt acattgcgca aatgaggggc gcacgaaatt
     3841 gctgcgcgcc cagtagtaat ctttcaattt tagcaaatgg ctttcttctg cattttcgct
     3901 tttgtgtccc ccacatcagc gtaatgaatg ttttgatcaa acagagggca aaaaaatagc
     3961 cataaaaaag taaaaatgcg gggcaacgcg caggacagct ctcgacgtcg ccctctgtgc
     4021 tgcctattat ttatggcggt gtcgtttggc ttgaagatca gaggaagaag agatgcgagc
     4081 agcgttttgg gtagggtgtg ccgctttatt gttgtcggcg tgtagtagtg agcctgttca
     4141 gcaggcgact gcggcgcacg tagcgccagg tttaaaagcg tcgatgtcca gtagtggaga
     4201 agcaaattgt gcaatgatcg gcggttcgct ttctgttgcc cgtcaactgg atggtacggc
     4261 gattgggatg tgtgcattac ccaacggcaa acgctgtagc gaacagtcac ttgccgccgg
     4321 gagctgtggc agctattaat tcattaaatc cgccagctta taagttaatg tctgtttcgc
     4381 ggtcgccagc gttaactggt tcgcggtcag atccacttgt gcaccttctt tcagcatttc
     4441 gctaatggtg ttatcgagtt cattaagctg cgggttagcg cacatcatac gggtcattgc
     4501 cagccctttg gctgtcagtt caccattaga cagtttgcct tcaccgctaa agcggttaca
     4561 catgctgccg gaaatcatca ttttttcacc aaagctgatt tctggcggat ttttatcgct
     4621 ggtcacgggc ttaccgttta cgctttccag cacaaagcga tgatgctgta gctgttctgg
     4681 cgtaacagca attttgtcat tacttacaca tcccgccatc agcaggctta gcgcaacaag
     4741 cgcggctact ttcttcattg tggttctcaa ttacagtttc tgactcaggg ctattttaag
     4801 aatagaggat gaaaggtctt tggggattat ctgaataagc tcccctggaa tgcaggggag
     4861 cggcaagatt aaaccagttc gttcgggcag gtttcgcctt tttccagatt gcttaagttt
     4921 tgcagcgtag tctgagaaat actggtcaga gcttctgctg tcaggaatgc ctggtgcccg
     4981 gtaaacagca cgttgtggca ggcagacagg cgacggaata cgtcatcctg gatcacgtcg
     5041 ttggatttat cttcaaagaa cagatcgcgt tcgttctcat acacgtccat acccaacgaa
     5101 ccaattttct gatttttcag cgcttcaatt gctgcctgtg aatcaatcaa tgcaccgcga
     5161 ctggtattga cgatcatcac gccatttttc atctgatcga aggcggcttc gttcaacaga
     5221 tggtagtttt ccggtgtcag cgggcagtgc agagagataa cgtctgattc agagaacagg
     5281 gttggcagat cgacatactc cacaccgagt tccagcgccg ctgcacttgg atacggatcg
     5341 aacgccagca gacgcatacc aaaacctttc agaatgcgca gcatcgccac accgatttta
     5401 ccggtaccga taacgcctgc cgttttgcca tacatagtaa agccggtcag accttccaga
     5461 gagaagttag cgtcacgggt acgctgatac gcgcggtgaa tacggcggtt cagcgtcatc
     5521 atcataccga tggcgtgttc agcaacggcc tctggatcat aggctggaac acggactact
     5581 ttcagcccca gttcttttgc cgcgtcaagg tcgacgttat tgaaaccggc acagcgcagg
     5641 gcgatatatt taacgccgtg ctttttcagc tcttccagca ccgggcggct gccgtcatcg
     5701 tttacgaaaa tacataccgc ttcgcagcca ttggcagttt tagcggtttt ttccgtcagc
     5761 agaaagtcaa aaaattccag ctcaaagcca aaggactcgt tcacctgttg caggtacttc
     5821 ttgtcgtact gttttgtgct ataaacggcg agtttcataa gactttctcc agtgatgttg
     5881 aatcacattt aagctactaa aaatatttta caaaatttca aatttaattg aaagctatgg
     5941 cgatattgaa aaattcatca acaactatgc ttagtgtagg cgcaaccttc aactgaacgg
     6001 ttaaacatgc cacaataccc gtattgaatg cttaattttt cgctaaatca ggatattaac
     6061 tacccatgct gggtaaatat aaagccgttc tcgcgctgtt attactgatt attcttgtgc
     6121 cgttgacgct gctgatgacg ctcgggctgt gggttcccac gctggcgggc atctggctac
     6181 cgctcgggac acgtattgca ttagatgaaa gcccacgcat tacgcgtaaa ggtttaatca
     6241 ttcccgatct ccgttatctg gtgggagatt gtcagcttgc gcatatcacc aacgccagcc
     6301 tttcacatcc cagccgctgg ttattgaacg tcggcacggt agaacttgat tctgcttgtc
     6361 tggcgaaatt gccgcagacg gagcaatcgc cagctgctcc aaaaaccctc gcgcagtggc
     6421 agtccatgct gcctaacacc tggatcaata tcgataaact gattttttct ccctggcagg
     6481 aatggcaggg aaaactctct ctcgcattaa cctctgatat ccagcaactg cgttatcagg
     6541 gcgaaaaagt taaatttcaa ggccagctga aagggcaaca acttacagtc agcgaactgg
     6601 atgtcgtcgc gtttgaaaat cagccgccgg ttaaactggt gggggaattt actatgccgc
     6661 tcgtgccgga tggacttcct gtaagtgggc atgctactgc gacgttaaac ttgccgcagg
     6721 aaccgtcact ggtgggtgcc gagctggact ggcaggaaaa tagcgggcaa ttgatcgtgc
     6781 tggcacggga taacggcgat ccgttgctcg atttgccgtg gcaaattacg cgtcaacaat
     6841 tgaccgtaag cgatggtcgc tggagctggc cgtatgcagg ttttcctttg agtggccgac
     6901 tgggtgtcaa agtcgacaac tggcaggcag ggcttgagaa cgctctggtc agcggacgac
     6961 tgagtgtgct gacccagggg caagcgggta aaggcaacgc ggtgctaaat tttggcccag
     7021 gaaaattaag catggataac agtcagctgc ctctgcagct gaccggtgaa gcgaaacagg
     7081 cggacctcat tttatatgcc cgtttacctg cgcaggtaag tggaagtctg tctgacccaa
     7141 cgctgacctt tgagccaggc gcgttacttc gttcaaaggg aagagtcatc gattcgctgg
     7201 acatcgatga aatccgctgg cctttagcgg gtgtaaaagt cacccaacgt ggtgttgacg
     7261 gacgtttgca ggccattttg caggcgcatg aaaatgaact gggcgatttc gtgctgcata
     7321 tggatgggct ggcgaatgat tttctccctg acgctggccg ctggcagtgg cgctactggg
     7381 gaaaagggag ttttacaccg atgaatgcca cctgggatgt cgcaggaaaa ggtgagtggc
     7441 atgacagcac gattacgctg accgatctct ccaccggttt cgaccagtta caatacggta
     7501 cgatgacggt agaaaagccg cgattaattc tcgacaagcc cgtcgtctgg gtacgtgacg
     7561 cacagcatcc ctccttcagc ggcgcgctgt cactggacgc cgggcaaacg ctgttcactg
     7621 gcggcagtgt gttaccgcca tcaaccttaa aatttagcgt cgatgggcgc gatccgacgt
     7681 atttcctctt taaaggcgat ttacatgctg gtgagattgg cccggttcgg gtaaatggtc
     7741 gctgggacgg tattcgtctg cgcggtaatg cctggtggcc taaacaatca ctgactgtat
     7801 tccagccgct ggtgccaccc gactggaaga tgaacttacg cgatggtgaa ctgtatgctc
     7861 aggttgcatt ttctgctgcg cctgaacaag gattccgcgc gggagggcac ggcgtgttga
     7921 aaggcggtag tgcctggatg ccagataatc aggtgaacgg tgtcgatttt gtcctgcctt
     7981 tccgttttgc cgatggagcc tggcatctgg ggactcgcgg ccccgttacg ttgcgaattg
     8041 ccgaagtgat taatctggtg acagcgaaaa atattacggc tgatttgcaa gggcgttatc
     8101 cgtggactga agaagaacca ttgctgttga ctgatgttag cgtcgatgtg ttaggcggta
     8161 acgtgctgat gaaacaatta cgtatgccgc aacatgaccc ggcgctgttg cggctgaata
     8221 atctctcatc cagcgaactg gttagcgcgg ttaatccgaa acagtttgct atgtctgggg
     8281 catttagtgg ggcgttgcca ctatggctga acaatgaaaa atggattgtc aaagatggct
     8341 ggttggcaaa cgcggggccg atgacgctgc gtctggataa agacactgca gatgcggtgg
     8401 ccaaagacaa tatgaccgct ggaactgcca tcaactggtt gcgttatatg gaaatcagtc
     8461 gctcatcaac aaggattaat ttagataatc tcggtgtgtt aaccatgcag gccaacattt
     8521 cgggcaccag tcgcgtcgat ggtaaaagcg gtacggtaaa ccttaattat caccatgaag
     8581 agaatatttt tacgttatgg cgcagtttac gctttggcga taatctccag gtatggctgg
     8641 agcagaatgc ccagctaccg gaaactggct gcccatcagg aaaagagtgt gaggaaaaac
     8701 aatgaaaata gtgatagccg cgtttgcgtc ctcttttatg ttggttggtt gcacgcctcg
     8761 cattgaagtg gctgcgccga aagagccaat caccattaat atgaacgtta aaattgagca
     8821 tgaaatcatc attaaagcgg ataaggacgt tgaagacctg ctcaaaaccc gtagcgatct
     8881 attttgaggt gatgatgaaa agaacattac tcatagctgt atgggccatt ggcctgatga
     8941 gcgacggtgc aatggcttta accctgaacg aagccagaag tcaggggcgg gtaggtgaaa
     9001 cgcttaacgg ttacctggtg gcgctacaaa cggatgccga aacacaggca ttagtaaaag
     9061 atattaatga agctcgtaac catagttacc aacaactggc aaagcaaaat aatgtatcca
     9121 ctaaagagat agccaaactg gctggacaaa agcttgttgc tcgcgcgaag tcggggcaat
     9181 atgttcaggg agttaatggt aaatggttgc gcaaataaat tatagttggc tacaaaatgt
     9241 atctattaat gatcttttac agtatttaaa ctgacgttag catgcttaat taccgtgggt
     9301 atttctacaa aaaccttata tatccttccc ttaaataaca a
//
LOCUS       DAZGBC010000177         9334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGBC010000177 DAZGBC010000000
VERSION     DAZGBC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.178"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..81
                     /gene="tnaC"
                     /locus_tag="ACHK1J_004248"
     CDS             7..81
                     /gene="tnaC"
                     /locus_tag="ACHK1J_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO7913446.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            302..1717
                     /gene="tnaA"
                     /locus_tag="ACHK1J_004249"
     CDS             302..1717
                     /gene="tnaA"
                     /locus_tag="ACHK1J_004249"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO7913447.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1808..3055
                     /gene="tnaB"
                     /locus_tag="ACHK1J_004250"
     CDS             1808..3055
                     /gene="tnaB"
                     /locus_tag="ACHK1J_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO7913448.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3187..4362
                     /gene="mdtL"
                     /locus_tag="ACHK1J_004251"
     CDS             3187..4362
                     /gene="mdtL"
                     /locus_tag="ACHK1J_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO7913449.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSQAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4337..5296
                     /gene="yidZ"
                     /locus_tag="ACHK1J_004252"
     CDS             4337..5296
                     /gene="yidZ"
                     /locus_tag="ACHK1J_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO7913450.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPSGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5453..6202
                     /gene="yieE"
                     /locus_tag="ACHK1J_004253"
     CDS             5453..6202
                     /gene="yieE"
                     /locus_tag="ACHK1J_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO7913451.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6224..6790
                     /gene="yieF"
                     /locus_tag="ACHK1J_004254"
                     /gene_synonym="chrR"
     CDS             6224..6790
                     /gene="yieF"
                     /locus_tag="ACHK1J_004254"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO7913452.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQEEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTNSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6844..8181)
                     /gene="adeP"
                     /locus_tag="ACHK1J_004255"
     CDS             complement(6844..8181)
                     /gene="adeP"
                     /locus_tag="ACHK1J_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO7913453.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8348..9013
                     /gene="yieH"
                     /locus_tag="ACHK1J_004256"
     CDS             8348..9013
                     /gene="yieH"
                     /locus_tag="ACHK1J_004256"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO7913454.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGINVDLEEIFT
                     RFKGVKLYEIIDIISKEHGVTMVQADAEHVYRAEVARLFDTELADIAGANELLASVNV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9150..>9334)
                     /locus_tag="ACHK1J_004257"
     CDS             complement(9150..>9334)
                     /locus_tag="ACHK1J_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913455.1"
                     /translation="NALNAKIVDCDDKIRSKLTIIRNNRLENQYKDDKNISDAMRNIL
                     DWFEQYSDIVKNITKA"
BASE COUNT         2297 a         2322 c         2320 g         2395 t
ORIGIN      
        1 tgcattatga atatcttaca tatatgtgtg acctcaaaat ggttcaatat tgacaacaag
       61 attgtcgatc accgcccttg atttgccctt ctgtagccat caccagagcc aaaccgatta
      121 gattcaatgt gatctatttg tttgctatat cttaattttt ccttttgcaa aggtcatctc
      181 tcgtttattt acttgtgtta gtaaatgatg gtgcttgcat atatacatgg cgaattaatg
      241 gatattgcaa atgtaatatt cacagggatc actgtaatta aaataaatga aggattatgt
      301 aatggaaaac tttaaacatc tccctgaacc gttccgcatt cgtgttattg agccagtaaa
      361 acgtaccact cgcgcttatc gtgaagaggc aattattaaa tccggtatga acccgttcct
      421 gctggatagc gaagatgtgt ttatcgattt actgaccgac agcggcaccg gggcggtgac
      481 gcagagcatg caggctgcga tgatgcgcgg cgacgaagct tacagcggca gccgcagcta
      541 ctatgcgtta gccgagtcag tgaaaaatat ctttggttat caatacacca ttccgactca
      601 ccagggccgt ggcgcagagc aaatctatat tccggtactg attaaaaaac gcgagcagga
      661 aaaaggcctg gatcgcagca aaatggtggc gttctctaac tatttctttg ataccacgca
      721 gggccatagc cagatcaacg gctgtaccgt gcgtaacgtc tatatcaaag aagcattcga
      781 tacgggcgtg cgttacgact ttaaaggcaa ctttgacctt gagggattag aacgcggtat
      841 tgaagaagtt ggtccgaata acgtgccgta tatcgttgca accatcacca gtaactccgc
      901 aggcggtcag ccggtttcac tggcaaactt aaaagcgatg tacagcatcg cgaagaaata
      961 cgatattccg gtggtaatgg actccgcgcg ctttgctgaa aacgcctatt tcatcaagca
     1021 gcgtgaagca gaatacaaag actggaccat cgagcagatc acccgcgaaa cctacaaata
     1081 tgccgatatg ctggcgatgt ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct
     1141 gtgcatgaaa gacgacagtt tctttgatgt gtacaccgag tgcagaaccc tttgcgtagt
     1201 tcaggaaggc ttcccgacat atggcggcct ggaaggcggc gcgatggagc gtctggccgt
     1261 aggtctgtat gacggcatga atctggactg gctggcttat cgtattgcgc aggtgcagta
     1321 tctggtcgat ggtctggaag agattggcgt tgtctgccag caggcgggcg gtcacgcggc
     1381 attcgttgat gccggtaaac tgttgccgca tatcccggct gatcaattcc cggcacaggc
     1441 gctggcctgc gagctgtata aagtcgccgg tatccgcgcg gtagaaattg gctctttcct
     1501 gttaggccgc gatccgaaaa ccggtaaaca actgccatgc ccggcagaac tactgcgttt
     1561 aaccattccg cgcgcaacat atactcaaac acatatggac ttcattattg aagcctttaa
     1621 acatgtgaaa gagaacgcgg cgaatattaa agggttaacc tttacctacg aaccgaaagt
     1681 attgcgtcac ttcaccgcaa aactgaaaga agtttaatta atactacaga gtggctataa
     1741 ggatgttagc cactctctta ccctacatcc tcaataacaa aaatagcctt cctctaaagg
     1801 tggcatcatg actgatcaag ctgaaaaaaa gcactctgca ttttggggtg ttatggttat
     1861 agcaggtaca gtaattggtg gaggtatgtt tgctttacct gttgatcttg ccggtgcctg
     1921 gtttttctgg ggtgccttta tccttatcat tgcctggttt tcaatgcttc attccgggtt
     1981 attgttatta gaagcaaatt taaattatcc cgtcggctcc agttttaaca ccatcaccaa
     2041 agatttaatt ggtaacacct ggaacattat cagcggtatt accgttgcct tcgttctcta
     2101 tatcctcact tatgcctata tctctgctaa tggtgcaatc attagtgaaa cgatatcaat
     2161 gaatttgggc tatcacgcta atccgcgtat tgtcgggatc tgcacagcca ttttcgttgc
     2221 cagcgtattg tggataagtt cgttagccgc cagtcgtatt acctcattgt tcctcgggct
     2281 gaagattatc tcctttgtga tcgtgtttgg ttctttcttc ttccaggtcg attactccat
     2341 tctgcgcgat gccaccagca ccactgcggg aacgtcttac ttcccgtata tctttatggc
     2401 tttgccggtg tgtctggcgt catttggttt ccacggcaat attcccagcc tgattatttg
     2461 ctatggcaaa cgcaaagata agctaatcaa aagcgtggtc ttcggttcgc tgctggcgct
     2521 ggtgatttat ctcttctggc tctattgcac catggggaat attccgcgag aaagctttaa
     2581 ggcgattatt tcctcaggcg gcaacgttga ttcactggtg aaatcgttcc tcggcaccaa
     2641 acagcacggc attatcgagt tttgcctgct ggtgttctcc aacttagctg tcgccagctc
     2701 gttttttggt gtcacgctgg ggttgttcga ttatctggcg gacctgttta agattgataa
     2761 ctcccacggc gggcgtttca aaaccgtgct gttaaccttc ctgccacccg cgttgttgta
     2821 tctgatcttc ccgaatggct ttatttacgg gatcggcggt gccgggctgt gcgccactat
     2881 ctgggcggtc attatccccg cggtgctggc aataaaagct cgcaaaaagt ttcccaatca
     2941 gatgttcacg gtctggggcg gcaatcttat tccggcgatt gtcattctct ttggtataac
     3001 cgtaattttg tgctggttcg gcaacgtctt taacgtgtta cctaaatttg gctaaatcct
     3061 tcaagaagcc agtcattcgc tggcttcttg cctctcagga aatcacttat gtccaaatgg
     3121 caactcgcct gatcctcctt caccacgtat gctttgcgtc accttactat caggacgctt
     3181 tagcccatgt cccgcttttt gatttgtagt tttgccctgg ttttacttta tcccgctggg
     3241 attgatatgt acctcgttgg tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg
     3301 cagttacata ttgcgttctc cgtttatctg gcaggtatgg cagctgcgat gttatttgcc
     3361 ggtaaagtgg ccgatcgttc agggagaaaa ccggtcgcca tccccggcgc ggcgctattt
     3421 attatcgctt cggtgttctg ttcacaggct gaaaccagta cgttatttct tgcagggcgc
     3481 tttctacagg ggttgggcgc gggctgttgt tacgtggtgg cattcgccat tttgcgcgac
     3541 acgctggatg atcgacgtcg ggcaaaagtg ctgtcgttac tcaacggcat tacctgcatc
     3601 attccggtgt tagcgccggt gctcggtcat ctgattatgc ttaaattccc gtggcagagt
     3661 ctgttctgga cgatggcaat aatgggcatc gcggtactga tgttgtcttt gtttatttta
     3721 aaagaaacgc gccctgccgc ccccgcagct tcggacaaat cacgagaaaa tagcgagtcg
     3781 ctgcttaatc ggtttttcct cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc
     3841 ctcaccttcg tcaacacgtc gccggtattg ctgatggaaa tcatgggttt tgagcgcgga
     3901 gaatacgcca ccattatggc gttgaccgct ggcgtcagca tgaccgtttc attctcgacg
     3961 ccatttgcgc tgggaatttt taagccacgt acgttgatga tcacctcaca ggtgttattc
     4021 ctggcggcgg ggatcactct tgccgtttca ccttcccatg cggtttctct gtttggtatc
     4081 acgctgattt gcgccggttt ctcggttggt tttggtgtgg cgatgagtca ggcgttaggg
     4141 ccgttttcat tacgcgcggg tgtagccagc tcgaccttag gtattgcgca ggtttgcggt
     4201 tcgtcactgt ggatttggct ggcggcggtg gttggtatcg gcgcatggaa tatgctgatc
     4261 gggattctga ttgcatgtag catagtgagc ctgttgctga ttatgttcgt cgcgcctgga
     4321 cgccccgttg ccgctcatga agaaatccat caccacgctt gatctcaatc tgctgctctg
     4381 tctgcaactg ctgatgcagg agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac
     4441 cccttcggcg gtgagtaagt cgctggcaaa gttaagagcg tggtttgacg acccgctctt
     4501 tgtgaactca ccgctggggc tgtcgcccac accgctaatg gtcagcatgg agcaaaatct
     4561 ggcggagtgg atgcaaatga gcaacctgct gctggataaa ccgcatcacc agacaccgag
     4621 cggcctaaag tttgagctgg cggcagaatc accgctaatg atgatcatgc ttaatgcgct
     4681 gtcgaaacag atctaccaac gttacccgca ggcgaccatc aaattacgta actgggatta
     4741 cgattcctta gatgccatta ctcgtggtga agtggatatc ggtttttccg gtcgcgaaag
     4801 ccatcctcgc tcgcgggagc tgttaagctc gctaccgtta gccattgatt atgaagtgct
     4861 gtttagtgat gtgccttgcg tctggttacg caaagatcat ccggcactgc atgaaacgtg
     4921 gaatctggac acctttttac gttatccgca tatcagcatt tgctgggaac agagcgatac
     4981 ctgggcgctg gacaatgtgt tacaggagct ggggcgcgaa cgcacgattg ctatgagcct
     5041 gccggaattc gagcagtcgc tgtttatggc agcgcagccc gacaatctgc tactggcgac
     5101 cgcgccgcgc tactgtcagt actacaatca actccaccaa ctgccgttgg ttgctcttcc
     5161 tctcccgttt gacgaaagcc agcaaaaaaa gctggaagtc ccttttaccc tgctgtggca
     5221 caaacggaac agccataatc cgaagatcgt ctggttacgg gaaaccatta aaaatcttta
     5281 cgcgtcgatg gcataaccga atcgtatgaa acgcgaccct atttcacaat aaaatgtaaa
     5341 aaatttgtaa taaacttgtc tgaatcgaac ttttagccgc tttagtctgt ccatcattcc
     5401 agtaaatgat tactcttgta ttcataatgg accattaagc atggagcgaa aaatggcgac
     5461 tcactttgcc cgagggattt taacggaagg acatctgatt tctgttcgtc tcccctccca
     5521 gtgtcatcaa gaagcccgaa acattccccc tcatcgtcaa agccgttttc tggcgtccag
     5581 aggtttactc gcagaactga tgttcatgct gtatggcatt ggcgaattgc cggaaatcgt
     5641 caccctgccg aaaggtaaac cggttttcag cgataaaaat ttgccttcgt tttctatttc
     5701 ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa ggtgaatgtg gcctcgatat
     5761 ggaactgcag cgtgcgacac gcgggtttca tagcccacac gcgcccgata accacacctt
     5821 ttccagcaat gaatcgctat ggatcagtaa acaaaacgat cctaacgaag cgcgggcgca
     5881 gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga atgacgatcc
     5941 gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa tgtgctcatg taaatcatgt
     6001 agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc gtggcggtca cgcccacgat
     6061 tgaaaagctc agtgtctggg agttagatgg caaacacggc tggaaaagcc tgccggatat
     6121 tcacagccgc gccaacaatc ctaccagccg gatgatgcgt tttgcccagc tctctaccat
     6181 gaaggctttt tcgccaaatt gatagacaac cacaggagtc attatgtctg aaaaattgca
     6241 ggtggttacg ttactgggga gcctgcgcaa aggctcattt aatggcatgg ttgcacgtac
     6301 cctgccgaaa attgctccgg cgagcatgga agtcaatgcg ttaccttcca ttgccgacat
     6361 tcccctgtat gacgctgacg tacaggagga agaaggtttt ccagcaacgg ttgaagctct
     6421 ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc acgccggaat ataactattc
     6481 ggtaccgggt gggctgaaaa atgccatcga ctggctttcc cgcctgccgg atcaaccgct
     6541 ggccggtaaa ccggtgttga ttcagaccaa ctcaatgggc gtgattggcg gcgcgcgctg
     6601 tcagtatcac ctgcgtcaga ttctggtttt cctcgatgca atggtgatga acaagccgga
     6661 atttatgggc ggcgtgattc agaacaaagt tgatccgcaa accggagaag tgattgatca
     6721 gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta ttcagcgagt
     6781 taagatctaa ataaaaaacc cgccagcaat catgcatggc gggtttttaa tgcgctatcg
     6841 attttaatga gcgtcgataa atacaatctt caggataaac agcagcgcaa cgatgattac
     6901 gcacgggcta aggtcacgga gacgcccggt accaatcttc atcacgcagt aggagataaa
     6961 gcccagcgca ataccttcgg taatcgagaa gctgaacggc atcatcacag cggtaataaa
     7021 cgccggaaca gattcagtaa gatcctgcca gttcacgcga gcaagacttg aggtcatcaa
     7081 cacgccaacg taaatcagcg cgccagctgc agcgtagcct ggcaccattc ccgccagcgg
     7141 cgacagaaag ataaccagca ggaacagcag accaacaacc actgccgtca gaccggtacg
     7201 accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag aagtaccgat
     7261 aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca tgcgcgggaa
     7321 tttccccttc tcatctgcca gacctgcttt atcggtcacg ccaatcaacg taccggagga
     7381 gtcaaacagg ttgaccagca tgaaagagaa aatcacccct gccagcccga ggttaaacga
     7441 cccggctaaa tctacatgac ccacgacagt cattacgctc ggcggcgcag aaacgatgcc
     7501 attgtagtgg acatcaccca gcatccagcc cagcagcgtt gtcaccacga tagaaaccag
     7561 caccgctgcg tgaatgttgc gtgaggccag aatagcaatg atgaagaagc cgaggatccc
     7621 cagcagcacg ctgtgagaag tcagattacc gatgctgacc agcgtttccg ggttagcgac
     7681 aatcacacct gcgtttttca gccccatcat gccaatgaac agaccgatac cgctggtaat
     7741 gcccacacgc agactcaccg gaatgttggc aatcatccag tagcgaacgc ggaaaatcgt
     7801 cagtaatagc agaccaatcg cgccccagaa gattgcgccc atcccgactt gccacggcaa
     7861 gcccatcgcc tgcacaacga caaaagcgaa gaacgcattc aggcccatag cgggtgccag
     7921 tgcaactggc aggttagcaa acagccccat cataatactg ccgaatgcag caatcagaca
     7981 ggtagtgacg aagacggcgc tggtatccat gccagcaacg ccaagaattt gcgggttaac
     8041 aaaaacgatg taaaccatcg tcaggaaggt ggtaaaaccg gcgatcactt cggtccgtgc
     8101 cgtcgtgcca tgttcgcgca gtttaaacac gcgttccagc atcccctggc cagaagcctg
     8161 ggttgtgtgt tgttgactca ttatctattt ccgaacaagg agggaaaatc cgtcgctatc
     8221 gtataccaaa atgagacaat atggacggct gaagataact ttttttcatt gtatttgctt
     8281 atacggcaac gattgcgtca tgcgaattgg catgacgaaa acgttaaact aacgataaag
     8341 gaaagacatg tcccagatag aagcggtatt tttcgactgc gacggtacgc tggtcgatag
     8401 cgaggtcatt tgctctcgcg cctatgtcac gatgttcagg gaatttggca ttaacgttga
     8461 cctggaggag atttttaccc gttttaaagg cgtaaaactc tacgaaatca tcgacatcat
     8521 cagtaaagaa catggcgtga cgatggtcca ggccgatgcc gagcacgttt accgcgcaga
     8581 agtagcgcgt ctgtttgata ccgaacttgc ggacatcgca ggcgcgaacg aattgctggc
     8641 gagcgttaac gtgccgatgt gcgtcgtctc taacggtccg gtcagcaaaa tgcagcattc
     8701 gctgggtaag ctcaacatgc tgcactactt cccggaaaaa ctgttcagcg gctacgatat
     8761 ccagcgttgg aagcccgatc cggcattaat gttccatgcg gcaaaagcga tgaatgtgaa
     8821 cgttgaaaac tgcattctgg tcgatgactc cagcgctggc gctcaatcag ggatagatgc
     8881 cggaatggaa gtgttttact tctgcgctga cccgcacaat aagccgatcg ttcacccgaa
     8941 agtcaccacc tttaccgagc tgtcgcagct gcctgcgctc tggaaggcac gcggctggaa
     9001 tataacgcga taatcgcctc cttacttcaa cgcttacaca tcagtcacgc tggtttgaga
     9061 taaagaacac caggtgtctg atgagtaagc gtctattccc tatgaataaa ttatgtcgtt
     9121 attcgtcctg tcatccaccc tctgcatagt taagccttgg ttatattttt tacaatatcg
     9181 gaatactgtt cgaaccaatc cagaatatta cgcatcgcgt cactgatgtt tttatcgtct
     9241 ttatattgat tctcaagtct attatttcga ataatagtaa gcttagaacg tattttatca
     9301 tcacaatcga caattttcgc attcaatgca ttta
//
LOCUS       DAZGBC010000178         9134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGBC010000178 DAZGBC010000000
VERSION     DAZGBC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.179"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..102)
                     /locus_tag="ACHK1J_004258"
     CDS             complement(<1..102)
                     /locus_tag="ACHK1J_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO7913456.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAI"
     gene            complement(360..2759)
                     /gene="dosP"
                     /locus_tag="ACHK1J_004259"
                     /gene_synonym="pdeO"
     CDS             complement(360..2759)
                     /gene="dosP"
                     /locus_tag="ACHK1J_004259"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO7913457.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(2784..4166)
                     /gene="dosC"
                     /locus_tag="ACHK1J_004260"
     CDS             complement(2784..4166)
                     /gene="dosC"
                     /locus_tag="ACHK1J_004260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO7913458.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            complement(4538..5857)
                     /gene="digH"
                     /locus_tag="ACHK1J_004261"
     CDS             complement(4538..5857)
                     /gene="digH"
                     /locus_tag="ACHK1J_004261"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO7913459.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5988..7523)
                     /gene="gadC"
                     /locus_tag="ACHK1J_004262"
     CDS             complement(5988..7523)
                     /gene="gadC"
                     /locus_tag="ACHK1J_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO7913460.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(7679..9079)
                     /gene="gadB"
                     /locus_tag="ACHK1J_004263"
     CDS             complement(7679..9079)
                     /gene="gadB"
                     /locus_tag="ACHK1J_004263"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO7913461.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT         2339 a         2241 c         2213 g         2341 t
ORIGIN      
        1 aatagcttta cctgtgatgt tatcagcgca ggcgtatttc aattcgatct ccagatcagg
       61 gaagatcacg gctaaatcaa ccagttcggt ggtatccgac ataaacggtt cctgtataag
      121 acaaaaattg ctgcgctttc cgcttatgca gatctcatgc catgccggga taagcgccag
      181 aatgctggct taaagttata ttttttaact tttgatcaac atttgtgcag cgtagtgcag
      241 ttttggtgca agaggggaag ttaaggaagg aatctcccgg aatcgtagct gaaatcacag
      301 tatttaagtg acagtgtcac gttaaatgaa aacccgcgag tgcgggcgag aggaatttgt
      361 cagattttca gcggtaacac gctgctcatc cagcctggaa tttcttcggc gggtaggggg
      421 cgggaaaaga aatatccctg aataactcga cagtggatct tacgtagcat ctcaaattgc
      481 tctttggttt cgacgccttc cgccacgacg gttaaattga ggctttgccc aatgctggta
      541 atggcttcaa gtaaggcaag gatgcgtttt tccgtcagac aacgatcgac aaaacttttg
      601 tcaattttga tttccgttac cggaagactg actaagcggg ataatccgga aaagcccgtg
      661 ccaaaatcat ctaccgataa acccacgccc atatcacgca ggatctgaat gcgcttaaag
      721 atttcggtat cgtgttccat catcatgctt tccgtgattt ccaccgtcag ctggtggccg
      781 tcaatacccc aggcttgcat tgcatcagac acctgattag gcagttgatt actgcgaaag
      841 tgcagcgccg acaagttcac ggataacgcc gggatatgaa tattctggct acgccattct
      901 gctaactgac ggcaagcttc cgcgatgacc cagcgcccaa tattttcgat ttcaccaatc
      961 tcttctgcga gaggaataaa ccgtgaaggg ggcacatgac catgctgggg atcgtgccag
     1021 cgagcaaggg cttcgatgcc gtacagttca cccgtttctg cgaagatttg cggctggtaa
     1081 accagtttca gttggttatt gctaatcgcc tctttcagcg ctgcgcctaa aaccaaacgc
     1141 tcttttacca tttcgttcat cgccgggctg aagaactgcc agccgttacc gccattcttg
     1201 cgaatataat ccattgcatt gtgagcagtg gagagcaggt aatcgcggtt tttacccacg
     1261 tcgtagctga tgccaatact caaggtaagc gggaagggtt tatcgtcaat cattatcggc
     1321 ttgctgacca cattccgtag ctcatcggcg atttgggtaa tgttactgac gtcgttttcc
     1381 aggctcacga ggacaaactg cgtaccttcg atacgacaga gatactgatc cggtttgagt
     1441 ttttcacgaa agcgattgac cacttccagc aatgcctgat cggcccacgc atagccaagg
     1501 ctatcaatca catcctgaat atggtcaaca ccgatgagat acaccaccgg agagacggct
     1561 ttgtcgacca ggtcatcgag gtaattgtgc aggttattgc gatttggcag accggtcatc
     1621 ggatcaaatt ggatgagttg ttcaatatgc tgacggcttt tttcctgttc cagcgccagc
     1681 gcggccatat gctggctgat atctgccacg cgttcgataa aggcgctggt ttctgctcct
     1741 gacgaggttt taatttgcag gatccccgca ggcgcgccat cacgctgacg aatggtcgct
     1801 gaccagcttt gcgcattttg aacttctgca ccgtgggaag atgacgccca gtgtatcggc
     1861 atcccgttgc gcagtgcgaa cagcgaaaca tgcgattcgt tgagaacaga ttcgatatta
     1921 cgacaaatga tttcccccat ttcatgaaat ggcgggctgc tgcacatggc ggcgagaata
     1981 ttgccttcaa gctggcggat ttgccgttct tcggtgatat ccgagaaggt cattaccagg
     2041 ttctgcagat gcgcgagcac gtcataaacc gggctgatag aagctttaat ccagattttt
     2101 tcaccggtgc gcgtcaacag cagaaattcg tcctgatcgc gggcggtttt ccatagcaac
     2161 tgttgtaaac gaatgcggtt atcggcaggg aattcaggaa tgttcaggag tgtatcgggc
     2221 tgcataccgc tggcttcgct aatgcagtaa ccaaacattt cggtaaatgc gcgattgcac
     2281 tgcacaatat ggcgttccgg atcgaggaca atcaccggtc ggtcgagatg gtcaacggca
     2341 ataatcaatt gcctggtctg ttctttttgc gccatttcta cgctggcatc ccgtaccagc
     2401 gccaggtaat aaactttccc ctcggcgctc acttttgata gcgcaaaacg ggtccagatt
     2461 ttactgccgt cttttttctc cagctgcagc tcccgactca tcccctcgac gcgcgcttta
     2521 ccgccttcac ggttatgacg aatgtattca ggatgcgcag gacgcaaatc ccgcggaatc
     2581 agcatatcaa tgttattgcc aatgacttct tcacgtttgt acccccagag cttctctgcg
     2641 gcggggttga aaaacatcac ttcatcattt tcgttaatta acaccgcacc catcatattt
     2701 tgctcaaggg cggggaaaaa aatgccatcg gcggcattat ccgcatcggt tagcttcatg
     2761 attacctctg catcctggcg catctaaaga ctggctttcc agagttcaac acggtttcta
     2821 cctcgtcttt tggcgatata cagagcttca tcggctattt gaatgaggcg ctcatagtca
     2881 ggatgaccat taaacatggc ggcaccgatg gaaagtgaga gggcaatatc ttcgccgttt
     2941 gcggctttca gtttggtttt ctccacccga ctgcgaatac gttctgcggt acgtaacgtt
     3001 tcgttttcag aagcttcagt caaaacaatg ataaattcat cgcccccgta gcggaaaaca
     3061 taatcactac tgcggacgtt gtcataaaag gcctgagaga ctttacgcag aatttcatcg
     3121 ccagtgttat ggccccacgt atcgttgatc tctttgaatt tatcaacgtc aataatcagc
     3181 actgacagcg gtgtaccggt ccggttggca tgggcaattt cgcgtttgaa gatagtcggc
     3241 aggaaacggc ggttaagtaa tttcgtcagt acatccatac cgacttcgtg gcgcgatact
     3301 tcttcaaaca attcacgcag caaggtaata atttgcgata cggtatttct tatctgtaat
     3361 aaaaatttca cccgcagact tctgttattc aaattcctgg tgttacgcat ggtttgattg
     3421 aaaataccgt cgaaatcctg aatcagacga gagatatggc ccacttcagc aatgccgctg
     3481 aaataatgtc gacctttatg gttaaaccac aggccaaaat cagcctggct taaaggcaaa
     3541 ctactgccta aatcagaatc cagcaggatt ttatagatga tatctatttc ccatgaaagt
     3601 attgaggcta tttgccgttc tttttcttct tcggcgtttt ccagtaacga gaagatacga
     3661 tagttttcat cttcttttga ggcactactg tcactaaagg taaacgcgcg ggtcatcact
     3721 tccatagcga tatcaatact gttaatcgag aaatggtaga cctgaagttt ttctgcggca
     3781 gaataatccg aagagaagat taccggatag aggatctttt tcagcacccg aaaccccatc
     3841 tcgacaattt ctaccggaat tcctatgcgg gcatgcactt ccgcgacggt atgctggatt
     3901 tgtattagcc tttcgacatc gtcaacctgg gcagaaagca cgttaataat ccagcgttcc
     3961 atcgcacttt tcaactgccg ctcaacttgt tcattactca agaattcttc ggcatgcggg
     4021 tcgatgcgga caattcgata aaactcaata ctcagataat gagcatgcgc aacggcaatt
     4081 tccgcggctt tagctcgaat gggcggatct gcctgttcga caagtccggt ccactcatct
     4141 ttcattcttt taaaatacat ctccataatt cacaccctta taaggctggg aaatcagacg
     4201 gaatcaaaac gcaacgtgcg agatcgacta actgcaccat attctcctga aatatgaaga
     4261 tatactgaaa agaaataagc gacttaggac agtttcaatc tacgctactg ttcttcagaa
     4321 gagtatagcc catcgtaatt atttttcggt gacagcgaat atcgtatgtt ttttcatatt
     4381 catacatttg tattagggga tttatggctg tttaactaag tgtggttaat tgcatggtta
     4441 atttgacttt agtaagcatg attagtagtg ggatagttca agagggtaac aagccggtgg
     4501 gtaatgtacc ggcttgttac aaagtaagaa tgggagttta actgccccaa cgactttgca
     4561 gatagctgac cgcttgttga gtctgcggtt tattcagata gtcctcacgg aacaagatgg
     4621 tgccgctaat ttctggtacc gcatcgttaa gatcgagctg ctttttcagt tccggtacgc
     4681 cgccgttaat catccagtct ggctctatct ttgaaggttc acccacttta tagaaggcga
     4741 taccgatata caggcgggtc ctggtcggtt taacgacatc cgcccaccat tttgccaaca
     4801 cgtcataacg cgcggcactc cgtgagaacg gccagtaaat ttggggagca atgtaatcca
     4861 gcaatccttg ttccacccat cgacgggtgt cagcgtagga ttcgtcatag gctgccgcgc
     4921 ctcgggtatc ggaaccgagc ggatcgtgtg atcggttacg ccacacgcct gccgggctaa
     4981 caccaaattc gactcccggc ttaatgcttt taatggtgtg cgatactttt gcaattaact
     5041 gctgagtatt gttgcgccgc cagtctgctt ttgacgcaaa tgcgcctccg tatttgcggt
     5101 acgtttcgtt atcatttagc cgtgaacccg gtgactcagt atagaaatag tcgtcaaact
     5161 gcacgccatc taccggatag cgggaaacca cttctgcgac tattgatgtg atccagtcct
     5221 gaacctcagg gatgcccggg tcgaggacaa agcgatcgcc agacgttctg atccagtcac
     5281 ggtgttgcac atagacgctc gccggttgtt gagacagagt gctattcagt tccctgatag
     5341 taccgggctt cgtattaacc gatacgcgat aggggttaaa ccaggcgtgt actttcatcc
     5401 cacgcttgtg ggcttcgtcg agcataaatt gcagcggatc gtaacccgga ttttcaccaa
     5461 tcttaccggt cataagatcg gaccatggca aaatttttga aggccacagg gcggtaccgt
     5521 ccggcttgac ctggaaaaag accgtgttta taccgagacg ttgcaaatga tccaatttgt
     5581 cgatcatcgc ctgttgttgt acacgggccc ggctggtggg gttactaatg ttaaccgagg
     5641 aaaccggtgg ccagtcgagg cgagacaccg tggccagcca gatgccacgc atcggttgtg
     5701 acgattgttg cgtcgtggct ggtggctttg aacccgctgg tggtgtcacc atggactctg
     5761 gaggcgtgct tttacaacta cacagtaaaa gtgccagtgc aaccagtatc gctggtcttc
     5821 taatcgttaa tttcttgttt cgggagcaga tatccatatg tgctggtttc cggtaaacag
     5881 atgtgcgctc attctcgtac ttatcccggt taagtcaata cgacagcaag cacgaaaaag
     5941 ggagcgatga attatcgctc ccttgtctta taaccattca gacatggtta gtgtttcttg
     6001 tcattcatca caatatagtg tggtgaacgt gcacgcgggt gcaggaagaa gtgacctttt
     6061 ggtgcgttct gactgttgat tggctccaga gtgacgccgg tattcgcttt gcctttacga
     6121 tcatgaacag catagagaat aaagggcagg gcaagtacca ccaggaaact aacgaccagt
     6181 aattcaacat acatatcggt agagtcaccc tggatattat ccggcggcag gaaggaaaca
     6241 ataaacgcca taattgaagt cagcagaccg acaatagcca cgaccagttt cacaccttta
     6301 ccaccaggga tattaaatgt gcgttttaag tcaggatgtt taagaaccaa cacaatgtag
     6361 ccaataaaca gcatgaaata agcacacaga taaatcacca ccgtcagcgc cagtgcgatc
     6421 aggaaggaca tgttgttacc gccaccggta ttggtgagga tgatcaacgc gatagaagta
     6481 atcaccagct gtgaaatgac cagcgttacc ggtacgccat ttttgttcat tttagcgaat
     6541 gccgctggca gcaggttttt ctgcgctgtg acatacatcc cgcgagaagg accaacaatc
     6601 caggaggcga tttccgccag aacacccagc aacagcagtg cggagatcac gcgaaccgtc
     6661 cactcaattt ccggtgccac atgggacatc agaacggtaa aggtttgcat tacccctgcg
     6721 gagaggttga tttcattacc cggaatgacc atcgcaatag acaaaccgcc aacagagctt
     6781 aagcagattg ccgccaccat cagcagtaac atagccagtg gatagtcgcg ccctgggtta
     6841 ctcatttcat tgacgtgggt tgcggaagct tctacgccca tataactcaa aatgaaggca
     6901 acaaatacaa ccagggtgcc cactttagag aagtcaggga agaaggtctt cgaatccatt
     6961 tcgatagcaa cgggggcacc ggagtgcaga taaatagccg ctaatgcaat caaaataaat
     7021 gcaggtaaca ggataccggc gaagaagcca actttagcaa ttcgcgccgt gtatttcgtg
     7081 ccaccaaact gcgttaatgc cagcgcccaa agaatgatga gtgctgcaat agttttggta
     7141 atggggtctt cattcagcgc tggccatttc agaatgtagg agagtgcccc taacacgaaa
     7201 tagagcatcg gaataaaacc aatggcgatt tgcagatagc caaatgagat cgctgcaaat
     7261 ccccatctcg gccccagagt atttgatacc caggcgaaga caccaccttc ttcccagccg
     7321 tcgacggtgg ccatttccgc agcacaaagt cccacgggaa taaaccataa aatcccgcct
     7381 aatagcagga agaagactaa tgaaaagccc gatgttgcga aggtagggta ttcataaaca
     7441 gccattacca tcgatgccgt tatggcaaaa aagccgagta atgtgagctg cttagcttta
     7501 cctgtctgta ctgatgtagc catattatcc ccctaaaacg gtattcctgt cggaaccgca
     7561 cctgttttgt tctgaagcgt attcagaaca atattttccg ttgctaatgc cagtgaacag
     7621 actttggaaa ttgtcccgaa acgggttcgt ttcgggacac cgttaccgtt aaacgttatc
     7681 aggtatgttt aaagctgttc tgttgggcaa taccctgcag tttcgggtga tcactgagat
     7741 atttcaggga ggctttgtag tcttccagca acagttcagc aaagtccatt tcgaagccgc
     7801 gacgacacat aatgcgcatc accacgatgt cggtggcttc accgccgaga gtgaaggccg
     7861 gaacctgcca gccgcgcaga cgcagacgtt cagagaggtc gtacagggtg tatcccggat
     7921 cttcaccctc tttcagtttg aagcaaaccg ccgggatgcc ttcgtccggg cgacccgtac
     7981 agatgaactc atacggcccc agtttggcga tttcatccgc cagataagcg gcaacctggt
     8041 aagaggcgtt ctgtactttg gtatagcctt cacgaccgag gcgcaggaat tcatagtact
     8101 gtgcaattac ctgacccgcc gggcgggaga agttgatggc aaaagtacca atttgaccac
     8161 ccaggtagtc aacgttgaac accagttcct gcggcagcgc ttcttcgtca cgccagataa
     8221 cccagccgca gcccagcgga gccagaccga atttatggcc tgaagcactg atcgatttca
     8281 cacgcggcag gcggaagtcc cagacgatat ccggggcgac gaacggtgcc aggaagccac
     8341 cgctggcggc gtcgatgtgc atgtcgatgt cgataccggt gtcggcctgg aatttatcca
     8401 gcgcatcgtg cagcggttgc gggaactcat agttaccggt gtaggtcacg ccgaaagtcg
     8461 gcaccacgcc gatggtgttt tcgtcgcagg cttcaatcat gcgtttcggg tccataaaca
     8521 actgaccggg gcgcataggg atctcacgca gctccacatc ccagtagcgg gcgaatttat
     8581 gccagcagat ttgtaccgga ccgcacacca ggtttggttt atccgttggt ttgcctgcag
     8641 cttccatacg cttgcgccaa cgccatttca tcgccatccc gccgagcata caggcctcgg
     8701 aagaaccaat ggtgttggtg ccaacggcct gaccattttt cggcgcaggc gcatgccaca
     8761 gatcggcaac catatttacg caacgcaggt cgatggctgc ggattgcgga tattcttctt
     8821 tgtcgatcca gtttttgtta atggataaat ccatcaactt gtggacattt tcgtcgtccc
     8881 aggtctggca gaaagtggcc aggttctgac gagcgttgcc atcaagatat aattcgtcat
     8941 tgataatctg aaatgcgaca tcgtcgcgca tttcgtgcag cggaaaacgt tttgattctg
     9001 cgatagtgga aatagacttc gcaccaaaac gtgaatcgag tagttccgac cttaaatccg
     9061 ttacttgctt cttatccatt ttaaactcct taaaatgatt ggatcgcatt aaaaaagtag
     9121 gatttatcga taaa
//
LOCUS       DAZGBC010000179         9114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGBC010000179 DAZGBC010000000
VERSION     DAZGBC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.180"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1512)
                     /locus_tag="ACHK1J_004264"
     CDS             complement(<1..1512)
                     /locus_tag="ACHK1J_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO7913462.1"
                     /translation="MNNTLPDDIEQLKALLIAQQAVIVRLSGEITGYAREISSLRALV
                     AKLQRMLFGRSSEKSREKIEKKIARAETRITELQNRPGEAQLQLTSMAGETAPKTSDS
                     PVRKAFPATLPRDRQVISPAETECPVCSGKLKPPGESISEQLDIINIAFRVIETVRPK
                     LACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKFAEHLPLYRQSEIYARQGVELH
                     RNTMGRWVDIMGEQLRPLYDELKHYVLMPGKVHADDTPVNVLEPGQGKTRTGRLWVYV
                     RDDRNAGSTMPAAVWFSYSPERKGIHPQQHLADYRGILQADAYAGYNALYESGQVTEA
                     ACMAHARRKIHDVHVRHPTTVTGEALRRIGALYAIESEIRGSPAEERLAVRKARTVPL
                     MQSLYEWLQGQMSTLSRHSDTAKAFTYLLKQWDALNEYCRNGWVEIDNNLCENALRVV
                     ALGRRNYMFFGSDGGGESAAVMYSLIGSCKLNGIEPETWLRHVISVINTWPANR"
     gene            complement(1532..1714)
                     /gene="tnpB"
                     /locus_tag="ACHK1J_004265"
                     /pseudo
     CDS             complement(1532..1714)
                     /gene="tnpB"
                     /locus_tag="ACHK1J_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1989..3008)
                     /locus_tag="ACHK1J_004266"
     CDS             complement(1989..3008)
                     /locus_tag="ACHK1J_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFO7913463.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            complement(3157..3321)
                     /gene="tnpB"
                     /locus_tag="ACHK1J_004267"
                     /pseudo
     CDS             complement(3157..3321)
                     /gene="tnpB"
                     /locus_tag="ACHK1J_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(3326..3994)
                     /gene="tnpA"
                     /locus_tag="ACHK1J_004268"
                     /pseudo
     CDS             complement(3326..3994)
                     /gene="tnpA"
                     /locus_tag="ACHK1J_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            4332..4473
                     /locus_tag="ACHK1J_004269"
                     /pseudo
     CDS             4332..4473
                     /locus_tag="ACHK1J_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4567..5793)
                     /locus_tag="ACHK1J_004270"
     CDS             complement(4567..5793)
                     /locus_tag="ACHK1J_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7913464.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(5866..6888)
                     /gene="iolG2"
                     /locus_tag="ACHK1J_004271"
     CDS             complement(5866..6888)
                     /gene="iolG2"
                     /locus_tag="ACHK1J_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFO7913465.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFTLRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(7061..9001)
                     /gene="iolD"
                     /locus_tag="ACHK1J_004272"
     CDS             complement(7061..9001)
                     /gene="iolD"
                     /locus_tag="ACHK1J_004272"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFO7913466.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT         2317 a         2456 c         2015 g         2326 t
ORIGIN      
        1 gcggttggct ggccaggtgt tgatgacact gatcacgtgg cgtagccacg tttccggctc
       61 gattccgtta agtttgcagc taccgatcag gctgtacatc actgccgcac tctcgcctcc
      121 tccatcagag ccgaagaaca tgtagttacg ccgccccagt gcaactaccc ggagggcgtt
      181 ttcacacaga ttattgtcga tctccaccca gccattgcgg cagtattcgt tcagggcgtc
      241 ccattgcttc agcagatagg tgaacgcttt cgccgtatca gagtgacgcg acagcgtgct
      301 catctgcccc tggagccact catacaacga ctgcattagc ggtaccgttc tggcttttct
      361 gaccgccagt cgctcttctg ccggactgcc gcggatctca gactcgatag cgtacagtgc
      421 cccgatacga cggagtgctt ctcccgttac tgtcgttgga tggcggacat gtacatcgtg
      481 gatcttgcgt cgggcatgtg ccatacaagc cgcttcggtt acctgaccgc tttcgtaaag
      541 agcattgtaa cccgcatatg catcggcctg caggatacct ctgtagtccg ccagatgttg
      601 ctgcgggtgg atacctttgc gctcgggaga gtatgagaac cacaccgctg ccggcatggt
      661 cgaaccggcg ttgcgatcgt cacgaacata gacccacagc cgtccggtac gggttttacc
      721 ctgacccggc tccagtacat ttaccggcgt gtcatcggca tgcactttac ccggcatcag
      781 cacatagtgc ttcagttcat catacagcgg gcgaagctgc tctcccatga tgtcaaccca
      841 gcgccccatc gtattgcggt gcagctccac gccctggcgg gcatagattt ccgactgacg
      901 gtacagcggc agatgctcgg cgaacttcgc catgattatg cgggccagca gagccggact
      961 ggcgtaactg cgctcgatgg gttttggtgg ctgcggagcc tgaactatac agtcgcaccg
     1021 gctgcaggcc agttttgggc gaaccgtttc gattaccctg aacgcgatgt tgatgatatc
     1081 cagttgttca gagatgcttt ctcccggcgg tttcagtttg ccgctgcaga cggggcattc
     1141 ggtttctgcc ggggagataa cctgcctgtc acggggaagt gttgccggaa atgctttgcg
     1201 gacgggagag tctgatgttt tcggcgctgt ctctccggcc attgaggtga gttgcaactg
     1261 cgcctcacca ggcctgttct ggagctcggt tatacgcgtt tctgcccgtg cgatcttctt
     1321 ttctatcttc tcgcggcttt tctcgctgct gcgaccgaac aacattctct gcagtttagc
     1381 gaccagcgct ctgagcgagc tgatctcgcg ggcatagccg gttatttcac cagacagacg
     1441 gacgataacc gcctgctgtg cgatcagcag ggccttcagt tgctcgatgt cgtcggggag
     1501 tgtgttgttc attcccctgt tttatcacgg gttatatccg gatgccaggc cgttctgtcc
     1561 gtttgggatg ttgccacgcg atcccctcca gtagcatgga taactgagct ggcgtcaggt
     1621 gcactttccc ttcccgggtt accggccaga cgaagcggcc ccattccagg cgtttggaga
     1681 acaggcataa cccgtcacga tcggcccaca gtatttatat gaacgcatcc cgtttgcaag
     1741 gcatccattg ttttgacatg acgggtaggt tgcagcttta tatccggctt tattgcagcc
     1801 gtaacgctgc gagcccctat gtaattcgcc gactcacccc ttattttcac ggcggacttc
     1861 aagcccaaaa tactgttcag gtttagtgag tcacggtctt acctgtcttt gtcatcactc
     1921 tggccgccat agcaaccttt cagcattcac ccttttgtta agagttcatt gtttaacggt
     1981 atcagcgatt aatatggtct gatactgacg tggttcctgt catacttacg gtcatgggcg
     2041 gttatcgccc atactgttcg tgccagcttg tttgccatgg cgacgattgc cacactggct
     2101 ggacgacgtt ttttcagttc ggttatccac gggccaggct ctttagctac taatgccacc
     2161 gctcttgcac cgtggataaa taatgtcctg agataagtgt caccacgttt gcttatcccc
     2221 agcagacgta ctttccctcc ggagccagtt tgttttggaa ccagaccaac atatgcggcg
     2281 aactctcgcc ccgatttaaa cgctgatgct tcccccatgg tggccaccgc tgccgtcgca
     2341 ataagtggtc caacgccagg aatatccagc aaccgcttac aggtttcatt ctgcctcgcc
     2401 acgctggtaa gctgtttttc aatatcctct atcagtgagt ccagctcatt cagtcggttg
     2461 tactggtttt ccagaaccat aatgagatac ggtggcagtc tctctttcat ccgttccagt
     2521 gcttcgggga actcccgctc catcgctgcc cggcctttgt ggatggtttc accaaactcc
     2581 agtaacgtcc cgtgcagggc attaatttgt gcggtccgga acttcaccag ttgcatgcgg
     2641 gtacggtgca gaaccagtac cgactgctgt tcttctgttt ttacggcgat ttctttaccc
     2701 ggctgctgaa ccgccatcca gatagccctg gcatccatca catcattctt attgcccatg
     2761 acgaatgcct taacgaagcg ggcctgcaac aaccggactt tatgaccaag ttttgtcagt
     2821 tcccgtgccc agtgctgaga acctccacag gcctccatac caatcaggca tggctcacgg
     2881 ttgccgaaga acgtcagaaa atcctgtcta cgcaactgtt tatcaaccac ttcacctgtg
     2941 tgctcattga tgaagtgaat ctgaatgaca tgttttgcga tatcaacgcc aaccggtgta
     3001 tatttcattt gtggagtctc cagtctgagg gagctttgtg catcccgtat tgggcactat
     3061 gatgccggaa atctgcgagg ctccacacct gccagtttca cccttttaac catcatactg
     3121 ctccgtgttt aattgtgtgg gatgcgttca ttacattttc accattttgc cactgcggcc
     3181 ccggaagacg aagatatgcc cggagaacgg gtcatctttc agcgtgttct gcaccttcga
     3241 agccaggccg ttgaagccac aacgcatatc tgtgatgcca gcgatgatcc agattttggt
     3301 accggtcggc agcgttatca tcagatcccc ctttcatttc gcgaatgagc accctaagaa
     3361 gttccggagt gaggggggaa aacagtttta ccacacctga tttaagatgc agctcacact
     3421 gtggcgagcc tacggtagtg tcattctggc tttcatcaga cttgtttcgc cagaaaggat
     3481 ttgtcaccgg tttgacgggg tcaagagtct cagtgattgc caccggaacg gcctggcatt
     3541 cctggagatc ctcatcattc agtaagccct cttcatacag tcttttccag ttaaacaaca
     3601 gattgtcatt aatattgtgc tctctggcga tccgtgcaac gactgcacca ggctgtaatg
     3661 cctgttttac cagcctgatt ttaaattcac gactgtagga ggttcgtcgt tcttttcgcc
     3721 atgtgctttc gctgatttga ggatcatcag gtaattcctt ctttctattg gcataaagta
     3781 tggcatcgag ttgggcgact gaaagtgatt caggtaatgg ccaagtaata ccggaggcat
     3841 taaatcgttg aaagagatta tgaatggtgg aatgactgag acctaactgc agggcgatag
     3901 cccggacaga taattgctcg tcgaatctta gacgtaatgc gtctggcaaa taagagcgaa
     3961 agccggtgat atcttttgct gtctgagaat tcatcgttcg tgtccatcaa tatagatgga
     4021 cgcgattgtt gccagacagg acaaccatca caagacgtca caggcagagc gcttacggaa
     4081 tggccttcca accgtgttga cgaactgctg ccatggaacg tagttctcac cgataaataa
     4141 gcgtcaatac ggtgctctgt tgacgcttac cttttactgc gaaaatggac attagtatcg
     4201 gaaacaggaa agggaggcga aagacggttt aaatgatgcg cttacccgca gggttgaaat
     4261 aaccggggaa acatcggtga ctgattaaca atgaagtgcc cccggagtaa ttccgggggc
     4321 cgtaaccgga atttattttc tttggctgtg cttatgaaag tgaatgagga gccctgctgt
     4381 acggggtgag tctcatgctt ccagaagcac atctgttata ttctgagcgt tctgccagaa
     4441 tggccttcct gccgtgttga tgaactcctg tagaacgaag tacttcgcca ataaataacc
     4501 gtcaatacgg cattcgatta atgcacttac agctaaatgg tgtttttgaa caatatttga
     4561 cgttatttat ttctcaccag gaactttaag aaactgaacc aggatgccac taaataaata
     4621 gagtcctgcc atagtataca taaccacgcc tgtaccaaaa cctgtcagaa tggataccaa
     4681 cgcaggtccg acaaaatttc ccaggccaga accgaaattc accgctgagt ttgctgcccc
     4741 cttcttatcc ggtgccatca tcggtaccag cggcgtcatc ggcacaaatc ccgccagccc
     4801 cgctccccac aggcacattg ccagcataaa agcaacgtaa ttatgccccc acactatcgg
     4861 cacccagtag acaatcagcg tggcaaaccc ggtcagaaga ctgccaaaaa tctggatagt
     4921 acggcgaaaa cctattttat cccccatcca gccgaaaata atgttgaaaa tctgattaga
     4981 aataaaggtt ataccccaga tactcgccca ctcctcaagg gtaaactgtt tatccagcaa
     5041 aaacagcggc aacaccacta caaaaccata ggtgccgagt ccattgattg ttttaacaat
     5101 cactgccaga ccaattcggg gtcgttgata cataatggaa atacctgccg acaactcttt
     5161 aaagactgac gtagttcttg gcttcagaat gaacgcattg cgctcaaaga ccagcatgaa
     5221 aatcccccca agcagtgcaa ccccaatccc ggcccacata acattaatat gcccaaggac
     5281 aggaattaac gaagcagcta ctaccggacc aatgatggta aaacctaagt tatatactac
     5341 ccaaaacagc gacgttccaa ttccctgttg atgattatcg agttgaatgg tcagtcggac
     5401 aagaaaggaa tagcagacca acggataacc tactccccgc agcatatagc taatgagaat
     5461 tgtactaaaa tcatggctgg gaatcgcaaa attaagaaac gcgatagaag cgatcaggta
     5521 aacggagaaa ccagcaagca taacctttct ggggccaaac atatccacac agacaccgga
     5581 taaccaggaa gttacggcaa ccacaattcc gtaggcggca aatatctgtc cgatctgaga
     5641 ctgatcaaat ccctgagtat tcagccaggc agatagccaa ctattttcaa tacagccgcc
     5701 caacataaat aaaaatgttc ccaggtagct ccagataaac tgacctggct ttatataaaa
     5761 tgtctgaggc aacattgcct gcatatcttt cattttataa ccctcagtac ggataatctc
     5821 tcgctcgtaa tagtgcgaga gacccttcgc tatttacaaa tgcaactaaa gttgaatatc
     5881 agtttttatc ttcactggca gtccactttt taatgattgt tttgcagcaa tggcaatacg
     5941 ctgcgccaat aatgcatctt caccagtaca gacaatgggt gtatgattca gaacagaatc
     6001 aataaagtgt aatatttccg ttctataagc ttcacgatag cgtagcgtaa aatcaggcaa
     6061 aggatttttc atgatacagt tctgactgtt atataccttc accgccgatt cactgacatt
     6121 gtcggcatac gccatccctg ctgaaccaaa gacctcaacc cgttggtcgt agccataaac
     6181 cgcccgacga ctattatcaa ttagcgcata accaccgttt ctgaaggtca gcatcaccac
     6241 gagagtatct acgtcatcaa tatttttcag agtaggatca atcaatgtat tgcctttaac
     6301 ataaacctcg ctaacttcat cctgcataat aaagcgagcc atatcaaagt catgcatagt
     6361 aaaatcaaat atgagtccac ctatcctctt gatcaaatcg tgcggcaaca actctggatc
     6421 acgtgaggtg atctttatca tctgaggctg accaatcttg cctctgcgca ccagttcata
     6481 cacctcccga aactggggat caaaacgacg attaaaagca atctgcagtt tgactccttt
     6541 ctctttaacc gcccttagca ccgcaaggga agctttctca tcttctctca tgctgagagg
     6601 tttttcgcag aaaatatgtt tacccgcctg gactgctgcc gtaacgatct cttcgtgcat
     6661 atctgtcgag gtgaaaataa acaccgcctc aatatcatca cgctgtaaca tttcctgata
     6721 atctttatat cctgaggtta tatttaccga actcagccag tcgctcattt cctcaataaa
     6781 actgtcagca gcacagacaa tatctaattg aggaagtaaa tacatattct caacatgcat
     6841 tttccctacc cggccaagac caataatccc acatcgaagc ttttgcattt atacctcgtc
     6901 atatcatgat atagtaaata taaaacgaca aaagaaaatc ccctttatcc aacacggtac
     6961 gaaagacttt attttgtagt ttcaacgaac aaatactttt accgtaaaaa acgtcaggtt
     7021 gcagaaaggc gccctcctgc aaccttaaaa actgaattaa ttagtactgt cgcgcttgtc
     7081 caatatgctc atcaagcata cgagctactg cctggatgcg ttcgctacgc gacacgcgag
     7141 cgccaccaac acgccaccag ctcagatatt tatgcgccat tgttttaggc agtactttaa
     7201 tgtctatcaa cgtactcacc gtctgtttac gtgcatcctc cagagcttca agcagttgtc
     7261 caatgctggt gacgcgatag cttttacagc catatccttc ggcaatccgg gcaaaatcca
     7321 caggaatgag tcccccctcc tgacgaccgt tatcctgttt atggaaacga aactcggtaa
     7381 agaaactgtc cataccgtgt tccatttgaa gattatttat acaaccatta gtcatgttat
     7441 ccagcaaaat aacattaatt ttcttcccca tctggaccga agttaccagc tcagaatgca
     7501 gcatcataaa tgagccgtcg ccaacgaagc tatatacctc ccgctccggt tgcgccaact
     7561 tcgcccccaa tgcggcattg acctcatagc ccatacagga atagccatat tctacgtggt
     7621 aatcattatc gccacggctt tgccagatgc gctgcagatc gcctggcaga cttcccgccg
     7681 cagcaaccac cagcgcgttt tctggcagca ttcgatttag tactccaaga acgcgcgtct
     7741 gtgtcagcca tgaatgagta atttcattga actcttcaaa cactttttga gtatccatat
     7801 gatccacaat ttccggtttg aaatccttac cgctgtattc cgctgcatag acgcgatcca
     7861 cttccgccag atattgagaa cgaacacgat ggatcgtttt cccccattgt gatatgtaac
     7921 cttccggagc cagtcgttcg caaagctgcg tcagggcaac acgggcatca gcctgcaatt
     7981 gcactccatc cagtttgaag acgtcaaaac ggctgacgtt tatattcaga tagcgcacat
     8041 caggattctg gaaaatccat ttagaagagg ttgtgaaatc ggtataacgc gtacccacac
     8101 caataatcag atcggcctcc tgagccagca aattcgccgc cagcgatccg gtttcaccaa
     8161 tcccaccaac attcagcgga tgcgcagaac tgagcgcacc tttaccagcc tgcgtttcag
     8221 caatcggcag atgatattgt tcggcaaatc gcagcagttc ctgttcagac tgcgaatact
     8281 tcacaccccc accgcaaata ataattggtc tctggtggcg aagaataaga gcaactgcat
     8341 cattcagcat tagctctgtt ggcacgtggc gctcaagacg atagacccga cgtgcaaaaa
     8401 aatagtctgg ataatcccag gcttctccct gaacatcctg cggcaaacaa agcgtcacag
     8461 ctcccgtatc agccggatcg gtaagcaccc gcatcgcgct aatgcaggcg ctcattagct
     8521 gttccggacg gataatgcga tcccaatagc gactcactgc acgaaaagca tcattggtgc
     8581 tcagcgtcag gtcatgctcc tgttcaatct gctgcaacac aggatcgggt tgacgcgatg
     8641 catacacatc tcctggtagc agaagcaacg ggatacggtt cgccgtcgca gtcgccgccg
     8701 ccgtgatcat attagcggca ccaggcccta cggaggaggt gcaggcataa atctgctgac
     8761 gacgcatctg acgcgcaaat cctgttgcta tatgtgccat accctgttca ttgcgtcctt
     8821 gatgaacgct aagttgacca cactcttctt ccagagcctg ccctaatccc acgacattcc
     8881 catggccaaa aattgcgaaa atacctttta caaacttgat ttcgcgaccg tccacttcaa
     8941 tgtactgatt atcaagaaag cgcaccaaag cctgcgccat agtcaacctg attgttttca
     9001 tgcactccac ctatcttatc aaccgtttga ctgtcgttac ctacgtacgc actgctacag
     9061 ccccgggcat aaaacactca ccacaagaaa aaaattccgc aaacgcaatt atat
//
LOCUS       DAZGBC010000180         9055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGBC010000180 DAZGBC010000000
VERSION     DAZGBC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.181"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..163
                     /locus_tag="ACHK1J_004273"
                     /pseudo
     CDS             1..163
                     /locus_tag="ACHK1J_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(344..1099)
                     /gene="mlaA"
                     /locus_tag="ACHK1J_004274"
     CDS             complement(344..1099)
                     /gene="mlaA"
                     /locus_tag="ACHK1J_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO7913467.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1393..2325
                     /gene="yfdC"
                     /locus_tag="ACHK1J_004275"
     CDS             1393..2325
                     /gene="yfdC"
                     /locus_tag="ACHK1J_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO7913468.1"
                     /translation="MDNDKIDQHSDEIEVESEEKESGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2401..2475
                     /locus_tag="ACHK1J_004276"
     tRNA            2401..2475
                     /locus_tag="ACHK1J_004276"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2434..2436,aa:Arg,seq:cct)
     gene            2637..3794
                     /gene="intS"
                     /locus_tag="ACHK1J_004277"
     CDS             2637..3794
                     /gene="intS"
                     /locus_tag="ACHK1J_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO7913469.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IISLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            4167..5120
                     /gene="ompT"
                     /locus_tag="ACHK1J_004278"
     CDS             4167..5120
                     /gene="ompT"
                     /locus_tag="ACHK1J_004278"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO7913470.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            5307..6791
                     /locus_tag="ACHK1J_004279"
     CDS             5307..6791
                     /locus_tag="ACHK1J_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7913471.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKPENIYCPGNSEAEIQ"
     gene            complement(6975..7187)
                     /locus_tag="ACHK1J_004280"
                     /pseudo
     CDS             complement(6975..7187)
                     /locus_tag="ACHK1J_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            7337..8005
                     /locus_tag="ACHK1J_004281"
     CDS             7337..8005
                     /locus_tag="ACHK1J_004281"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7913472.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(7950..8087)
                     /locus_tag="ACHK1J_004282"
     CDS             complement(7950..8087)
                     /locus_tag="ACHK1J_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO7913473.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(8060..8644)
                     /locus_tag="ACHK1J_004283"
     CDS             complement(8060..8644)
                     /locus_tag="ACHK1J_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7913474.1"
                     /translation="MAFRMSEQPRTIKIYNLLSGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(8644..>9055)
                     /locus_tag="ACHK1J_004284"
     CDS             complement(8644..>9055)
                     /locus_tag="ACHK1J_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913475.1"
                     /translation="SSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTHSV
                     TGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         2673 a         2066 c         1967 g         2349 t
ORIGIN      
        1 ctttattcgg taacgaagtt aacgttgacc attctcaaaa aatatataag cactttaagg
       61 aactcgaagg taacattgaa aaaggcttca agatcgccag acgaaatatc aaaatgaaat
      121 taatcaacta tatgcgctag gtgttaattt tgatgatatt taaattattt ttccagtttt
      181 ttgatttttc aacagctgaa tatcggaaat agtcagtgac gaacatcagt atttttacat
      241 aattacgtcc gggtcattgt cggtttatct ccttttactt gtgataattg agggataaca
      301 acaaaaaagg tgagtattgc gactcacctt ttttatttgt ttcttattca gaatcgatat
      361 cttttaaatc atcctgaatc gcttgcgcgt tcggattttc ctgcggtttg agttcgccgc
      421 cattagcgat gaaatcatga cgctggaagt acgcttcgcg caccataata taaggatcgg
      481 acgactgacg cagcagaccg tcggaatcca acaactgcgc acgagtttct atcccttcaa
      541 gcgtccattt acccacagac atcggccagg tcagccagga tagaaccggg taaagaccat
      601 ccgccatatc accaccgtca tcacgcagcg tgaagctacc gtagaacggt aactggacgt
      661 aaggcccata acccacgcca taatgaccaa gcgtactacc gaagcggtga ggttcagtcc
      721 gttgcagttt cgggttcgcc atccctgcaa catcaataaa accacccatc cccaaaatgg
      781 tgttcaggaa aaagcgggta aagtggacca tcccctgata agggtcgccc tgcaagaagt
      841 agttcaccat caccgcgggt tcttcaaggt tgccggtaaa gttgctcaaa ccgttacgcg
      901 ccggttgcgg aacataatca cgccaggcga cagcgaccgg tcgaacaata tacgggtcta
      961 gtacgttgaa gttgaagttg tacatggtgc ggttgaaccc ttctaacggg tcagaacgcc
     1021 cttgctgatc tgtaccggaa ctcgcacacc ccaccagaag cgtagttccc agagcaagcg
     1081 ccgacaggcg aagcttcata aatgtctccc tgttttttta tggcttatgc agtttgccat
     1141 ccatgacgga acgataccgt atccgcctgt ttaggtgtgg gcgattgtaa cagcacgtca
     1201 actgatgtcc agacgccctg atttgctgat ttgatcatag cctggtaatc gccgccctgt
     1261 aggctacttg attctataga aacagaaaaa ggcaaacgcg gccttttcta cattttcaga
     1321 gtaactcccg ccagttgcga gcgaaaaagc cgctacgctt tagctatacg tgctaattca
     1381 agagaagaga ccatggacaa cgacaaaatt gatcaacaca gcgacgaaat tgaagttgag
     1441 agcgaagaaa aagagagtgg caaaaaaata gaaatagatg aagatcgact cccctcccgg
     1501 gcgatggcaa ttcatgagca tatccgccag gatggtgaaa aagagctgga acgcgacgca
     1561 atggcactac tgtggtcagc cattgcggcg ggtctgtcga tgggcgcttc attactggca
     1621 aaagggatat ttcatgtcga actggaaggt gtgccgggca gcttcttgct ggagaatcta
     1681 ggttatacct ttggttttat tatcgtcatt atggcccgcc agcaattatt taccgaaaac
     1741 accgtgactg ctgtactacc cgtcatgcaa aaaccgacaa tgagcaacgt tggcttactt
     1801 atgcggttat ggggcgtcgt gctgctgggt aatattctcg ggacaggtat tgcggcgtgg
     1861 gcatttgaat atatgccaat cttcaatgaa gaaactcgcg atgcatttgt caaaatcggc
     1921 atggatgtga tgaagaacac ccccagcgaa atgtttgcca acgcgatcat ttccggctgg
     1981 ctgatcgcca ctatggtatg gatgtttcct gcagcgggtg cggcaaagat tgtggtgatt
     2041 atattgatga cctggcttat tgccctgggt gacaccaccc acatcgttgt cggttctgtt
     2101 gaaatcctct atctggtgtt taacggcacg ctgcactgga gcgatttcat ctggcccttc
     2161 gcactaccta ctttagcggg gaacatctgc ggcggcacct ttatcttcgc gttaatgagt
     2221 catgcacaga tccgtaacga catgagcaac aagcgcaaag cagaagcacg ccaaaaagca
     2281 gaacgtgcgg aaaacattaa gaaaaatgat aaaaacccgg cataaatggc gagggtttaa
     2341 gcaatcgcac ggcagcgtac ttaccccgca gtccattagc gggtatactc attccgcatt
     2401 gtcctcttag ttaaatggat ataacgagcc cctcctaagg gctaattgca ggttcgattc
     2461 ctgcagggga caccatttat cagttcgctc ccatccgtac cagtccgcaa aatcccctga
     2521 atatcaagcc ttccgtagat tcacagttcg taatggttcg cgtcagatcg ttgacagccg
     2581 cactccatga cgggtaaaaa gtggataaaa taattttacc caccggattt ttacccatgc
     2641 tcaccgttaa gcagattgaa gcagcaaagc cgaaagaaaa accataccgc ctactcgatg
     2701 gtaatggcct gtacctttat gtccctgtgt cagggaaaaa ggtatggcag cttcgctaca
     2761 agattgacgg taaggagaaa atcctgaccg taggaaaata tccgctaatg actttgcagg
     2821 aggcaagaga taaagcatgg accgcgagga aagacatctc ggttggcatc gatccggtaa
     2881 aagcgaaaaa ggcttcgtct aacaacaatt cctttagtgc gatttacaag gaatggtacg
     2941 agcacaagaa gcaagtctgg tcagtaggct atgcaactga acttgcaaaa atgtttgatg
     3001 acgacatttt acctatcatc ggcggccttg aaattcagga tattgagccg atgcaactgc
     3061 tggaagtaat ccgcagattt gaagatcgcg gtgcaatgga gcgagccaac aaagcccgca
     3121 gaagatgcgg cgaggttttc cgttacgcta ttgtcactgg tagggctaaa tataacccgg
     3181 cacctgacct tgctgacgcc atgaagggat accgcaagaa gaacttcccg tttctcccag
     3241 cagaccagat ccctgcattc aacaaagcac tggcaacatt ttcaggaagc atcatatcgc
     3301 tcattgcgac caaagtttta cgctacacag ccctaagaac gaaagagctt cgctccatgc
     3361 aatggaagaa cgtcgatttt gaaaacagga ttatcactat cgacgccagt gtgatgaagg
     3421 gccgcaagat tcatgttgtt cctatgtcag accaggtggt tgaacttctc actacgctaa
     3481 gctccatcac taaaccagta tcagagtttg tttttgccgg gcgcaacgat aagaagaagc
     3541 caatctgcga gaacgcggta ttgcttgtga tcaaacaaat cggctatgag ggtctggaaa
     3601 gcggtcacgg attcaggcat gaattcagca caattatgaa cgagcacgaa tggcctgctg
     3661 acgctattga agtgcaactg gcacatgcca acggcggatc tgtgcgtggg atttacaacc
     3721 atgctcagta tctcgataaa cgcagagaaa tgatgcaatg gtgggcggac tggattgatg
     3781 aaaaggtgga gtgatccacc ttaaccacta tcgaagagca caaagccttg caatccagta
     3841 caaagctatg tgtgtctcag ttttgtccct cttttttgta ctaaaaaaca tagcaattga
     3901 ggataaacct catgctattt tcgcttatat gcctttaaag gcatggcgct taaatagata
     3961 aaaacaccac aaaagcataa aaaaccacac agtaaaaccg caacataaaa caataacaga
     4021 taattaaacc aaaaacagat agcgcattgt gataatcatt caatgctaaa caaaatataa
     4081 acagtggagc aatatgtgat tgactcatta agttagatat aaaaaataca tattcaatca
     4141 ttaaaacgat tgaatggaga acttttatgc gggcgaaact tctgggaata gttctgacaa
     4201 cccctattgc gatcagctct tttgcttcta ccgagactat atcgtttact cctgacaaca
     4261 taaatgcgga cattagtctt ggaactctga gcggaaaaac aaaagagcgc gtttatctag
     4321 ctgaagaagg aggccgaaaa gtcagtcaac tcgactggaa attcaataac gctgcaatta
     4381 ttaaaggtgc aattaattgg gatttgatgc cccagatatc tattggggct gcgggctgga
     4441 ctactctcgg cagccgaggt ggcaatatgg tcgatcagga ctggatggat tccagtaacc
     4501 ccggaacctg gacagatgaa agtaggcacc ctgatacaca actcaattat gccaacgaat
     4561 ttgatctgaa tatcaaaggc tggctcctca acgaacccaa ttaccgcctg ggactcatgg
     4621 ccggatatca ggaaagccgt tatagcttta cagccagagg tggttcctat atctacagtt
     4681 ctgaggaggg attcagagat gatatcggct ccttcccgaa tggagaaaga gcaatcggct
     4741 acaaacaacg ttttaaaatg ccctacattg gcttgactgg aagttatcgt tatgaagatt
     4801 ttgagctagg tggcacattt aaatacagcg gctgggtgga agcatctgat aacgatgagc
     4861 actatgaccc aggaaaaaga atcacttatc gcagtaaagt caaagaccaa aattactatt
     4921 ctgttgcagt caatgcaggt tattacgtaa cacctaacgc aaaggtttat gttgaaggcg
     4981 catggaatcg ggttacgaat aaaaaaggta atacttcact ttatgatcac aatgataaca
     5041 cttcagacta cagcaaaaat ggtgcaggca tagaaaacta taacttcatc actactgctg
     5101 gtcttaagta caccttttaa cgaagttaac cagattttct ccccggtggt attgtataac
     5161 cccggggaat ttgtttacag ttacaagtag gcaatatcca ctaattttgt aagcatttaa
     5221 cctatgtcat aacgtgtaat ctcttaccgc aacacaagac ctcaatatat tcaaatctta
     5281 tctttattcc gatattcaga gacacaatgc gaaaaataat tactcatttc aaagttgttt
     5341 taacgttact tctaccagta accgtatctg cccagcagat acagtggcaa tcctgtatgg
     5401 ccagtcaatt caaccactgg tttggtgagg aaaaaccgtc tcctgactta ctatgtggtt
     5461 atttgtctgt tccattaaaa tatacagaca caggcggaga tgcttcttat gaaaaaaaat
     5521 cacaagtcaa actagcgttg acaaaattgc cggcaaaaag caagcataaa ggaagtatcc
     5581 tgataataag tggtggtccc gggttaccag gcataaatcc ttatattaac tttgactggc
     5641 cagtcacaaa tcttcgtgag tcatgggata ttattggatt tgatcctcga ggcgtcggac
     5701 agtccactcc gacaataaac tgccggcaat cagatacaga gactcaggaa aacataaccg
     5761 aaaagcaaca agtattaaat aaaattaatg cctgtatcca taataccgga gccgaagtca
     5821 ttcgccatat cggctctaac gaggctgtat acgatattga tcgtattagg caagccttgg
     5881 gggataaaca actgacagcc gtggcgtatt cgtatggaac tcaaattgca gccttatatg
     5941 cagaacgttt tccctacaac gtaagatcta tcgttcttga tggagtcgtc gatatcgatg
     6001 acctggagga caacttcaca tggcaactca aacaggcaca gagttatcag gaaacgtttg
     6061 atcgctttgc atcctggtgt gcgcgtacaa aaagttgccc gctttcttca gacagagata
     6121 aggcaataac tcagttccat gagctattat caaaattaca tcacaaacct ttattagaca
     6181 gtaagggaga aaatatatct tcagatgaac tcatatcatt aacaacagac cttctgctat
     6241 ggcgttcatc atggccaacc cttgcaactg ccatacgcca gttctctcag gggattgtca
     6301 gtaatgaaat tgaaactgcg ctcagtgctc cgatagcctc agaagagtca agcgatgctt
     6361 cgggggtaat cctctgtgta gatcaggggg atgagcaatt aacaccagaa gagcgaaaat
     6421 cccgaaaaga cgctcttgcg aatgccttcc cggctattaa ctttgacaat ggacgttccg
     6481 attcacctga tttttgtgaa ttatggccaa tacatagcga cctgaacaaa actcgcctga
     6541 aaaatactgt tctgccctct ggtttactgt ttgtagcaca caaatacgac ccaacaacgc
     6601 cctggattaa tgcccgtaag atggcagaga aattttccag cccgttacta acaataaatg
     6661 gtgatgggca tacattagct ctcaccggag ttaatttatg tgtagataaa gcagttgtac
     6721 atcacctgat cactccacaa aaaccagaaa atatatactg cccaggaaat tctgaagcag
     6781 aaatacaata atttcaaaat attccgctat ttgctcccaa tgcaaaacat atattgcatt
     6841 gggagcatat tcatattttt gttattcttg tatgaaatcg tctcatgtac acggtccttt
     6901 tttcgccgaa aactgatatt actttaatca aaagttgacg tgccagattc gaagtaaatt
     6961 tataaaatat caaatcaata tacattttgc accttcgaga tagtaatggc tgttactgta
     7021 acatgagtta caaacagctt catacccaga gctactgatg tatcaactcg attcaacaat
     7081 acccgatatt tattccatgc ctccagcaac gatctttctt cctccgttgc gatttccaga
     7141 tctacagcat cctgcagtgg cgcaatatac tcactgaatt cctggatgta gaactgtgtg
     7201 gtgacggtct tccagccatt cggctcctgc tgtatcgaag cataccatgc tatttcaata
     7261 tcgctatgct gcggcagcat ttaacccctt gtaattcatc gccataattg atttaattca
     7321 caaataaaac tataacatgg tgaaattaat gaaaaaaaac acagatgatg gggctaaaat
     7381 ttacacacca cttaccctaa agctttatga ctggtgggtt ttgggagtat caaatcggct
     7441 tgcatgggga tgtcctacaa aggaacacct tcttccacac tttctggaac atttaggtaa
     7501 caaccatctg gatattggtg ttggaactgg gttttacctt actcacgtac ctgagagtag
     7561 tctgatatct ttaatggatt tgaacgaagc tagcctgaac gcggcatcta caagggctgg
     7621 ggaatcaaaa attaaacata aaattaacca tgatgttttt gaaccttatc ccgcggcgtt
     7681 acatggtcaa tttgattcca tttccatgtt ttaccttctt cactgcctgc ctggaaatat
     7741 atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgacg atggaactct
     7801 atacggagcc acaattcttg gcgatggagt tgtgcacaat agcttcggtc aaaaactgat
     7861 gcgcatttac aatcagaaag gcatcttttc aaacacaaaa gattccgaag aaggcttaac
     7921 acatatactc tcagagcatt tcgagaatgt taaaaccaag gttcaaggta ctgtagtaat
     7981 gttttccgct tcagggaaaa aatagcatcc aaccgcagca cgttcttgct taagacgtgc
     8041 tgcggcataa tcccaatgat tactccctga cagggttcgt aggccactca atatcaggtg
     8101 cagttgatgt atcaacacgg ttcagcaaca cccgatactt tttccaggct tccagcaaca
     8161 aggtttcttt ctccgttgcg atctccagat ccacagcatc ctgaagcggc gctatatgct
     8221 cactggctac ctgcatcagg ctgttttttg tttcttccgc ctcccggatc cggaacagtt
     8281 tttctgcttc cgtatccttc acccaggctg tgccgttcca cttctgaaac tccccttccg
     8341 gggataacca ggtgacattt tccggtaatg agccgagttc agaaataaat aacgcgtcgc
     8401 cggaagccac gtcataaacc gttttacccc gatgatcttc aacgagatgc cacgatgact
     8461 catcactgtt gaaaacagcc acgaaaccag ccggaatatc tggcggtgca atatcggtac
     8521 tgtttgctgg cagacctgta tgaggcggaa tatatgcatc accttcacca ataaattcat
     8581 tagttccgga cagcagatta taaattttta tggtccgtgg ttgttcactc attctgaatg
     8641 ccattatgca agcctcacaa tatagttaaa tgcgatgttt ttgacggtgt tttccgcgtt
     8701 accagcagcg ttaacggtga tggtgtgtcc atgtgagcca atcgcaaccg agtgcgtatg
     8761 agcaccaata ccgacagtat gtgcgtgagc acctgcagat gcagctgtgc cgctgacact
     8821 atgagtgtgc gctcctgcag cacttgtatt cacactggcc gcagacacta ccttatgtac
     8881 tgacccattt tgtgaccact ggctgactgc tgatgcgcct gtgacactat gagtatggtt
     8941 tccggcactt gcggctgtac cgctcacact gtgcgtatgc gccccgatgt tattcgtgga
     9001 tttagtgccg taatcaaacg acgatgtggt tttcgtcccc aaatccgtac tggat
//
LOCUS       DAZGBC010000181         8835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGBC010000181 DAZGBC010000000
VERSION     DAZGBC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.182"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(305..568)
                     /gene="rpsT"
                     /locus_tag="ACHK1J_004285"
     CDS             complement(305..568)
                     /gene="rpsT"
                     /locus_tag="ACHK1J_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO7913476.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            671..889
                     /locus_tag="ACHK1J_004286"
     CDS             671..889
                     /locus_tag="ACHK1J_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO7913477.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            897..1838
                     /gene="ribF"
                     /locus_tag="ACHK1J_004287"
     CDS             897..1838
                     /gene="ribF"
                     /locus_tag="ACHK1J_004287"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO7913478.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1881..4697
                     /gene="ileS"
                     /locus_tag="ACHK1J_004288"
     CDS             1881..4697
                     /gene="ileS"
                     /locus_tag="ACHK1J_004288"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO7913479.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4697..5191
                     /gene="lspA"
                     /locus_tag="ACHK1J_004289"
     CDS             4697..5191
                     /gene="lspA"
                     /locus_tag="ACHK1J_004289"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO7913480.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5279..5728
                     /gene="fkpB"
                     /locus_tag="ACHK1J_004290"
     CDS             5279..5728
                     /gene="fkpB"
                     /locus_tag="ACHK1J_004290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7913481.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5730..6680
                     /gene="ispH"
                     /locus_tag="ACHK1J_004291"
                     /gene_synonym="lytB"
     CDS             5730..6680
                     /gene="ispH"
                     /locus_tag="ACHK1J_004291"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO7913482.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6746..7660
                     /gene="rihC"
                     /locus_tag="ACHK1J_004292"
     CDS             6746..7660
                     /gene="rihC"
                     /locus_tag="ACHK1J_004292"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO7913483.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAVRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVEAQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALAP"
     gene            7827..8648
                     /gene="dapB"
                     /locus_tag="ACHK1J_004293"
     CDS             7827..8648
                     /gene="dapB"
                     /locus_tag="ACHK1J_004293"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO7913484.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGSIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLHNL"
BASE COUNT         2013 a         2190 c         2493 g         2139 t
ORIGIN      
        1 taggaattta attcaaaaat agagtggtct taagtttaat acctcaatgt gtatcacaat
       61 atccacattc tttgtagggt agtctggaga ttgagtagat attcttgttc agaatgtatc
      121 agccgatggt tctacgattc tttagccacg aagagttcag atagtgcaac ggcatgtctg
      181 caagaacctg caacttttga ctatctggca accggcagtg tgttctctca cgcatcacaa
      241 aagcagcagg cataaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg
      301 gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
      361 ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg
      421 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
      481 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
      541 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
      601 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
      661 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
      721 ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcagt ttcttcccgt
      781 gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
      841 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
      901 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
      961 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
     1021 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
     1081 tgtttgctac cgataaagcc ccggcacggc tgacccggct gcgggaaaaa ctgcgttacc
     1141 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt tttgcggcgt
     1201 taaccgcgca aaatttcatc agcgatcttt tggtgaagca tttgcgcgta aaatttcttg
     1261 ccgtaggtga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
     1321 aagctggcat ggaatacggc ttcgatatca ccagtacgca aactttttgc gaaggtggtg
     1381 tgcgtatcag cagcaccgcc gtgcgtcagg cgctggctga tgacaatctg gctctggcag
     1441 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     1501 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     1561 aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     1621 acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     1681 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     1741 atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     1801 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     1861 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     1921 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     1981 atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     2041 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     2101 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     2161 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     2221 gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg tgaatacgcg gcgacccagg
     2281 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     2341 acctgaccat ggacttcaaa actgaagcca atatcatccg cgcgctgggc aaaatcatcg
     2401 gcaatggtca cctgcacaaa ggcgcgaagc cggtgcactg gtgcgtagac tgccgttctg
     2461 cactggcaga agcggaagtt gagtattacg acaaaacttc tccgtccatt gacgttgctt
     2521 tccaggcggt cgatcaggat gcgctgaaag cgaaatttgg cgtaagcaac gttaacggcc
     2581 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     2641 ctattgcacc tgatttcgac tatgcgttgg tgcagatcga cggtcaggcc gtgattctgg
     2701 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     2761 gcacggtaaa aggtgcggag cttgagctgc tgcgctttgc ccatccgttt atgggcttcg
     2821 acgtcccggc aatcctcggc gatcatgtta ccctggatgc cggtaccggt gccgttcaca
     2881 ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg
     2941 ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
     3001 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc
     3061 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     3121 tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
     3181 cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     3241 cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga
     3301 tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtaccctc gaactgatgg
     3361 aagaagtggc taaacgcgtt gaagttgacg gcatccaggc gtggtgggat ctcgatgcga
     3421 aagagatcct cggcgacgaa gctgaccagt atgtgaaagt gccggatacg ctggatgtat
     3481 ggtttgactc cggatctacc cactcttctg ttgttgatgt gcgtccggaa tttgccggtc
     3541 acgcagcgga catgtatctg gaaggttcag accagcaccg tggttggttc atgtcttctt
     3601 tgatgatctc caccgcgatg aagggcaaag cgccgtatcg tcaggtactg acccacggct
     3661 ttaccgtgga tggtcagggc cgcaagatgt ctaaatctat cggcaacacc gtttcgccgc
     3721 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaactgact
     3781 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgcagccgac agctatcgtc
     3841 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccggcaaaag
     3901 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
     3961 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
     4021 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     4081 gtcagtacac cgccaaagcg gatagcgtgg cgcgtcgtag ctgccagact gcgctgtatc
     4141 acatcgctga agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag
     4201 tgtggggcta tctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
     4261 gcctgtttgg tctggcagac agtgaagcaa tgaacgatgc gttctgggac gagctgttga
     4321 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg
     4381 gctcgctgga agcggcagta accttgtatg cagaaccgga actggcggcg aaactgaccg
     4441 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
     4501 acgacgcacc tgctgatgct cagcagagcg aagtactcaa aggactgaaa gtcgcgttga
     4561 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     4621 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     4681 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     4741 tgtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     4801 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     4861 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     4921 tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa
     4981 gctaaacaat atcgcttacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     5041 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     5101 caccttcaac cttgccgata ctgccatctg tgtcggtgcg gcactgattg tgctggaagg
     5161 ttttttgcct tctaaagcga aaaaacaata ataaaccctg ccggataagg cctacagatt
     5221 gctgcgaaat cgtaggccgc atccggcaaa aatcctcaaa atataagagc aaacctgcat
     5281 gtctgaatct gtacagagca atagcgccgt cctggtgcac ttcacgctaa aactcgacga
     5341 tggcaccacc gccgagtcta cccgcaacaa cggtaaaccg gcgctgttcc gcctgggcga
     5401 tgcttctctt tctgaagggc tggagcaaca cctgctgggg ctgaaagtgg gcgataaaac
     5461 caccttctcg ctggagcccg atgcggcgtt tggcgtgccg tcaccggacc tgattcagta
     5521 cttctcccgc cgtgaattta tggatgcagg cgagccagaa attggcgcaa ttatgctttt
     5581 taccgcaatg gatggcagtg agatgcctgg cgtgatccgc gaaattaacg gcgactccat
     5641 taccgttgat ttcaaccatc cgctggccgg gcagaccgtt cattttgata ttgaagtgct
     5701 ggaaatcgat ccggcactgg aggcgtaaca tgcagatcct gttggccaac ccgcgtggtt
     5761 tttgtgccgg ggtagaccgc gctatcagca ttgttgaaaa cgcgctggcc atttacggcg
     5821 caccgatata tgtccgtcac gaagtggtgc ataaccgcta cgtggtcgat agcttgcgcg
     5881 agcgtggggc tatctttatt gagcagatta gcgaagtgcc ggacggcgcg atcctgattt
     5941 tctccgcaca cggtgtttct caggcggttc gtaacgaagc gaaaagccgc gatttgacgg
     6001 tgtttgatgc cacctgtccg ctggtgacca aagtgcatat ggaagttgcc cgcgccagcc
     6061 gtcgtggcga agaatctatt ctcatcggcc acgccgggca cccggaagtg gaagggacga
     6121 tgggtcagta cagcaacccg gaagggggaa tgtatctggt cgaatcgcca gacgatgtgt
     6181 ggaaactgac ggtcaaaaac gaagagaaac tctcctttat gacccaaacc acgctgtcgg
     6241 tggatgacac gtctgatgtg atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc
     6301 cgcgcaaaga tgacatctgc tacgccacga ctaaccgaca ggaagcggta cgcgccctgg
     6361 cagagcaggc ggaagtggtg ctggtggtcg gttcgaaaaa ctcctccaac tccaaccgtc
     6421 tggcggaact ggcccagcgc atgggcaaac gcgcgttttt gattgacgat gcgaaagata
     6481 tccaggaaga gtgggttaaa gaggtaaaat gcgtcggcgt gactgcgggc gcatcggctc
     6541 cggatattct ggtgcagaat gtggtggcac gtttgcagca gctgggcggt ggtgaagcca
     6601 ttccgctgga aggccgtgaa gaaaacattg ttttcgaagt gccgaaagag ctgcgtgtcg
     6661 atattcgtga agtcgattaa gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt
     6721 taccggcatt ttttatggag aaaacatgcg tttacctatc ttcctcgata ctgaccccgg
     6781 cattgacgat gccgtcgcca ttgccgccgc gatttttgcc cccgaactcg acctgcaact
     6841 gatgaccacc gtcgcgggta atgtctcggt tgagaaaacc acccgcaacg ccctgcaact
     6901 gctgcatttc tggaatgcgg agattccgct cgcccaggga gccgctgtgc cgctggtacg
     6961 cgcaccgcgt gatgcagcat ctgtgcacgg cgaatcggga atggctggct acgactttgt
     7021 tgagcacaac cgaaagccac tcgggatacc ggcgtttctg gcgattcggg atgccctgat
     7081 gcgtgcacca gagcccgtta ccctggtggc catcggcccg ttaaccaata ttgcgctgtt
     7141 actttcacaa tgcccggaat gtaaaccgta tattcgtcgt ctggtgatca tgggcggttc
     7201 tgccgttcgc ggcaactgca caccaaacgc cgagtttaat atcgctgccg atccagaagc
     7261 ggctgcctgt gtcttccgta gcggcatcga aattgtcatg tgcggtctgg atgtcaccaa
     7321 tcaggcaata ttaacccctg actatctcgc tactttaccg gagttgaatc gcaccggaaa
     7381 aatgctccac gccctgttta gccactaccg tagcggcagt atgcaaagcg gtctgcgaat
     7441 gcacgatctc tgcgccatcg cctggctggt gcgcccggac ttattcaccc tcaagccgtg
     7501 ttttgtcgcg gtagaagctc aaggcgaatt tacctcaggc acgacggtgg ttgatatcga
     7561 cggttgcctg ggcaaaccag ccaatgtaca ggtggcattg gatctgaatg taaaaggctt
     7621 ccagcagtgg gtggctgagg tgctggctct ggcgccgtaa cctgtcacat gttattggca
     7681 tgcagtcatt catcgactca tgcctttcac tgatatccct ccctgtttat cattaatttc
     7741 taattatcag cgtttttggc tggcggcgta gcgatgcgct ggttactctg aaaacggtct
     7801 atgcaaatta acaaaagaga atagctatgc atgatgcaaa catccgcgtt gccatcgcgg
     7861 gagccggggg gcgtatgggc cgccagttga ttcaggcggc gctggcatta gaaggcgtgc
     7921 agctgggcgc ggcgctggag cgcgaaggat cttctttact gggcagcgac gccggtgagc
     7981 tggctggtgc agggaaaaca ggcgttaccg tgcaaagcag ccttgatgcg ataaaagatg
     8041 attttgatgt gtttatcgat tttacccgtc cggaaggtac gctgaaccat ctcgcttttt
     8101 gtcgccagca tggcaaaggg atggtgatcg gcactacggg gtttgacgaa gccggtaaac
     8161 aggcgattcg tgacgccgcc gccgatattg cgattgtatt tgccgccaat tttagcgttg
     8221 gcgttaacgt catgcttaag ctgctggaga aagcagccaa agtgatgggt gactacaccg
     8281 atatcgaaat tattgaagca catcatagac ataaagttga tgcgccgtca ggcaccgcac
     8341 tggcaatggg agaggcgatc gcccacgccc ttgataaaga tctgaaagat tgcgcggtct
     8401 acagtcgtga aggccatacc ggtgaacgtg tgcctggtag cattggtttt gccaccgtgc
     8461 gtgcaggtga catcgttggc gaacataccg cgatgtttgc cgatattggc gagcgtctgg
     8521 agatcaccca taaggcgtcc agccgcatga catttgctaa cggtgcggta agatcggctt
     8581 tgtggttgag tggtaaggaa agtggtcttt ttgatatgcg agatgtgctt gatctccata
     8641 atttgtaacc acaaagtgtt tatggtgatg caaaagtaac gcatttaatt tattgaatat
     8701 aaagggcttt aatttttagc ccttttattt ttggtgttat gcttttaaat tttatgcaaa
     8761 cgctataaat cacatgtttt gtcttctatt tttgttgttt taatgtaaat tttgaccatt
     8821 tggtccactt ttttc
//
LOCUS       DAZGBC010000182         8669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGBC010000182 DAZGBC010000000
VERSION     DAZGBC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.183"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(324..590)
                     /locus_tag="ACHK1J_004294"
     CDS             complement(324..590)
                     /locus_tag="ACHK1J_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO7913485.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(587..1354)
                     /gene="yjiL"
                     /locus_tag="ACHK1J_004295"
     CDS             complement(587..1354)
                     /gene="yjiL"
                     /locus_tag="ACHK1J_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO7913486.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            complement(1364..2515)
                     /locus_tag="ACHK1J_004296"
     CDS             complement(1364..2515)
                     /locus_tag="ACHK1J_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO7913487.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQREEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2631..3911)
                     /locus_tag="ACHK1J_004297"
     CDS             complement(2631..3911)
                     /locus_tag="ACHK1J_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO7913488.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3952..5184)
                     /gene="mdtM"
                     /locus_tag="ACHK1J_004298"
     CDS             complement(3952..5184)
                     /gene="mdtM"
                     /locus_tag="ACHK1J_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO7913489.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSMLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5651..6595
                     /locus_tag="ACHK1J_004299"
     CDS             5651..6595
                     /locus_tag="ACHK1J_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7913490.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            complement(6837..8249)
                     /locus_tag="ACHK1J_004300"
     CDS             complement(6837..8249)
                     /locus_tag="ACHK1J_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7913491.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            8426..8590
                     /locus_tag="ACHK1J_004301"
     CDS             8426..8590
                     /locus_tag="ACHK1J_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO7913492.1"
                     /translation="MEFHKNKAKTPFIRLVLLWHAMRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
BASE COUNT         2273 a         2272 c         2169 g         1955 t
ORIGIN      
        1 agggagccca tatttttatc cttgaattgt gaaatgttct tctaaatcta aaataatcag
       61 ggggatgggt aaagataagc ttcgcacaga aacttatctt ttatcattat ctcacccggt
      121 aatcatttta tttctctatg cataattatg ggtttaagta taataaaaag tattttatat
      181 tttagacgta aaatagtaag gtgctatcaa aaatcgctca ttttgtggat gggaaatatt
      241 tcacggcagg cgagatattt actacaactt ccttcatgca ggattatgcg cagccaatta
      301 tccggttggt atatttaaag atcctattgc ctggttgacg cttctgcatc gtaatgcacc
      361 acgcactgcc agtcgctgcc ctgctggcaa tatatcgctt cggtttgccc agggatcacc
      421 cattgtattt cctcgccggg gggacaggtc agccaaatgc ataacccgca gccgccgcgt
      481 aaatcacggg gaatatcgct gacgcgaaaa gtcatgcccg ctgcctgcaa cgccttgcgg
      541 gtttgtatga cgccgaccgt ggagtgaaat aaaaataaaa actctttcat cggcgtgtcc
      601 tcactcgttg accaattacc gccgcgccaa ttgcgccagc aaattgcgca tcaggatggg
      661 tatttaccgg cattcgcagg tgagattcca gcatccgggc aaacgtctgg caatgactaa
      721 cgccaccagt aaacagaatc ggcgcttcac aggagagacg agcaatgaaa ttggcactcc
      781 gccgcgccat cgcgttaatc actccggcga gaatcgcttc tggcgcgacg cccgctgagc
      841 gcaggctgat cacttctgat tcagcaaaca ctgtgcacat actcgtgatg gcgtgcggcg
      901 tgacattttc ggtaatgcag tcgagttgct cgacgctggt gccaagcgtg cgcgagatca
      961 cctccaggaa acgcccggtg cccgccgcgc atttgtcatt catcaggaaa tcgcacaggt
     1021 taccgtcatc atcaagctga atcactttgc tgtcctgacc accgatgtcg attaccgcgc
     1081 gcgttgctgg cgcaagaaac cgtgcgccca gcccgtgaca ggagatttcc gtcacttgtt
     1141 tatcggcaaa atctaccagt tgccgcccgt agccggtgag cgtcagaaac ggcgttgtct
     1201 ctaacccttc gcgcagagtt tcccaggctt cagtaattgc tgttgccggg cgaaaggggg
     1261 ttggaacgag gaaacggcgc gtaatcacgc cgtctgccag taagatccct ttggtggcgg
     1321 tagaaccaga atcaatgcca atcgaatatg tcactgccac tccttacagc atctcaataa
     1381 aggccgcgac acgggtactg agctgcccga catctgaggt ggagtagtct gtttcaatag
     1441 cgatataagg aatgttgtgc tgctgacgca catgacgttt aattgccagc gattccaccg
     1501 cgtaggtatg gcacgcctgc aaaatcacat caactacgcc atcgacctga tattcttcca
     1561 ccatctggct gagcattttc aggcgctgat cgttcggcga aacacaggag cagccaatcg
     1621 ccagatattt atccgccagc gcgtcgtaga catcgcccgt ttctgccacg cattgctcgg
     1681 tcgctttcgc cccggtgcag ttttcataac cgacaaccca gccgccattc tcttcaatcg
     1741 cgcgcaccac tttctctgct gcgccgccaa tcgggcagcc ggtgattaaa atgcgcggac
     1801 gcgggtccag tcgctggcct tcttcccgct gctgacgaat gcgggcggtc atcgcgtcca
     1861 gttcattgat caacgcctct ttatcgaacc ggaaggttgc gccgtaaacc actttcagaa
     1921 tgtcgctgcc gctaagcgcc ggaggattta actgcccaag atgataaaaa ttggccagtg
     1981 cgcgacgttc gcggtttttc agcgcaatag catcgcgcag agcatcttcg ctaatctcgt
     2041 gcccaaaacg ttcttctacc gttttttgca agcgcagcat ctcggctttc cacaatgcac
     2101 gcgaggcatc gtccttaacg ctgttcggca gctgcatcac atgaacaggc ttaaactccg
     2161 ccatgtattc atacattttc tttttgccgt cgcaggtggt ttcaccgacc accagatccg
     2221 aaaagtagaa gtaggggcat ttatcggttt tgccgaagcc gtagctgctt ttaatcagcg
     2281 ggcagaggtt gcgcggcaga tctttctccg cttcttcaat ggtttcatca gaggtggaac
     2341 agagcgaaac cacaaccgca ccggctgcca tcgggatctc ttgcggcata aaggtgcagt
     2401 aagtgccaac cagcggaatg ccgcgctcct tgagatccat gacggtgaga aagcctttct
     2461 ggcgagcttc agagaactga tcgaaaatgg cgggtagatc ggtgacaagt gacatgattt
     2521 tccttccccg taccacggga gataatgaaa agaggccaca ttattaccat tcttttagta
     2581 tggtttattt gatctctctc gcggttagcc acttagtttt tcatggattt ctaaaaattg
     2641 ccgaggggga acaaggccgg gagctgcgac agcaaatata aaattagccc aatacaaccg
     2701 ccaaccagcg taccgttgac acgaataaac tgcagatctt tgccgatatt taactctatt
     2761 tgccgcgaca tatcccgggc atcccagctt tttaccgtat cgctgatgtg gcgcgtcagg
     2821 aatgcggaaa actcaggcgc gacgcggtgc gcggcttgtt ccagatgacc atttaacgac
     2881 gcccgcaagg catcatcggc aattaacgtt tcgccaaacc attgacccgc ccgcgcgata
     2941 cgttctttca cacgagaatc ttcactgttg atatccgctt tcagccattc ccgtaaatcc
     3001 ccccacaatt cactgagata gcggttaaaa gcttcatctt ctttcagata actttttacg
     3061 gcatcggctc gcgctgccat ttccggatcg tttttcagct tgtcgatcag ggcaaaagtg
     3121 gcgcgatcaa acgcatggcg gatctgatgc gcacgatcgc ggctgatatc atcaagcaaa
     3181 gaattcaccg cgtcagaaac caactccgcg ctatgttcgc ccagccattc ggtggggaga
     3241 attttggctt tcagtggatg ctcactctcc agccagcgaa caatttgctg ggcgataaac
     3301 ttgcgcgatt tatcgcgctg gagaagagca atcaactgtg cgatcagcgt atccagcagc
     3361 acctgatgac gatcgttttt ggtcatactc tccagcatca acgcactggt gccggaaaga
     3421 tcgaccttat caatcgcccg atggaccgcg cgcttaagca ggcgctgaat acgcgcatca
     3481 tcagttaatt cgagaaaacc actcatgatc tgcaacagat gctgaccaac gcggcgggcg
     3541 ttttctggct ggctaaacca gttgccaatc aacaacgccg gttcgtggcg tcgaatcaat
     3601 gccaccaggg attgagtatc aagaaatttt tcctgcacga actggccgag attttcgcca
     3661 atccggtctt tattacgcgg gataatcgcc gtatgacgag aaataatcgg tatcggcacg
     3721 cggcgaaaca gcgccaccac cgcaaaccag tccgccagcg cgccgaccat cgccgcttca
     3781 gcaatcgcct tcacgccgct cacccaaaaa ttgggcggca aaaacagcgt aacgacaaag
     3841 gtagcagcgg cgataagcag taaagagagc gccaacattt tggcgcgtct gagttcaatg
     3901 agtttattca taacgttaag aatagagcct gacggcagga tcgcgcaaaa atcactgctc
     3961 ctccgccagc tcggcagcct gatgttggcg cacgcgattg agcaatcctg ccagggtgaa
     4021 aacgacgata acgcccgcca cgacggctaa cagatgaaac ggcaagcgac cgccgttaaa
     4081 ccacagccag cggccgattt cgaccgagac agacatcacc atcaggatca ccatattcag
     4141 cgatgcggag acggtccctt tcggtaagtt attggaaaac agcgtaaagc ggaataaggt
     4201 cgggaaaatc aaaccaatcc cgaaagcata cagactggtg cccagcatcg accacagcca
     4261 gacgtgcggt gacagcagat tgccgataat caacagcgcg aggccaacca gttgaatggg
     4321 tacggcacgc cagataaagc gcggttcggt cggatcttta acgaaacgcg ccacgatggc
     4381 attcgcgaca atcaccgcgc cgaacaccgg aacctgtgtc caggcgaact gcgaggtcgt
     4441 taagccgcct gcgtcaatca ggatcaccgg cgagacagcc acccaactca tcatggggat
     4501 atagcttaaa gagatggttg ctgcgccaaa gaggaacagc cgattgcaaa agacattacg
     4561 aaaatcgcgc aagacgcttt tggcgctaaa tggaaccgcg ccgcgcttca ccgtctctgg
     4621 catcgccagc agtaagccaa caaatgagat aaaacccata accgcaatga tggcaaaaag
     4681 gactttccag tgcacaaagt gcatcagagc tgctccggaa agcgggccaa taatcggcgc
     4741 aatcagtacg atggaggtga taatcgccat caacttgatc ccttttgtct gtccgaacgc
     4801 ctcctgcacc gtgacataac caacggtagc aataaaacag atactggtgc cctgaattgc
     4861 acgcgcgata agaaactgtg tcatagacgt tgtgaacatt gtcgcagcgc aggcaagggt
     4921 aaaaatgagc gccccggtaa tcagcactgg cctgcggcca atcctgtcgg aaagtggccc
     4981 cagcagccac tgtaacgcca taccgccagc gagatagaga ctgacggcag caggggccag
     5041 actgacatcg gcattaaaat cacggaccac attaatgatc cccggctgga tcagatccgt
     5101 cgaaagatag gcggcaaagt catacaaaat caacgccatc gggaaaaaca gcgtagcggc
     5161 atggcgggca aaaaaacgtg gcataacaaa caactccttg tccggagaac cccaatgggg
     5221 aaaacgaaat tcaataccgt cggtaatgac gaaaggcagg cgagaaggac aaatgtactg
     5281 aatttcatgt aatcaatcac actgcctgtg aacacacaga cagagaaata ccttctgact
     5341 tcgggtcaga atgtataacg attacaaata gtcatcagca cattacacct cttgaattga
     5401 tgggttgcat agaaaatata caccttaagt gtaattaaaa tttgcggtta atcaaaagag
     5461 cgcggtggaa aggggaatat ctgccgggta caggacatgt aaaacgcgag cctgtttccg
     5521 gaaaaatgta tcgtttgcag acgaacaaaa atagcctgga aagcacctcg gggaacgaga
     5581 aattgccggg tgagaatggt tttgttagtc gctaaagtcg ggccatctta tctacaggtg
     5641 acggatcgcc atgacaaact tcacgaccag cacgccacat gacgcattat ttaagtcctt
     5701 tctcatgcac cctgacaccg cgcgggattt tatggagatc cacttaccca aagatttacg
     5761 tgaactgtgc gatctcgaca gcttaaaact ggaatccgcc agctttgtcg atgaaaaatt
     5821 gcgggcgcta cactccgata ttctgtggtc ggtaaagacc cgtgaaggtg atggttatat
     5881 ttacgtagtg attgaacatc agagccgcga ggatatccat atggcctttc gcctgatgcg
     5941 atattccatg gcggtgatgc agcgccatat cgagcatgat aaacgccggc agctaccgct
     6001 ggtcatcccg atgctgtttt atcacggtag ccgtagtcct tacccctggt ccctgtgctg
     6061 gctggacgaa tttgccgacc cgactactgc acggaaactt tatactgcag cgttcccgct
     6121 ggtggatgtc actgtcgtgc cagacgacga gattgtgcag caccgcagag tcgccctgtt
     6181 ggagttgatc caaaagcata ttcgccagcg cgatctgatg gggcttatcg atcaactggt
     6241 aatattactg gttacagagt gtgctaatga cagccagata actgcgctgt taaattacat
     6301 tttactgact ggcgatgaag cgcgttttaa tgagtttatc agtgaactta cccgtcgaat
     6361 gccacaacaa agggagcgaa taatgacgat tgcagagcga attcataatg atggatggct
     6421 gttagggatg gaaaagggga aagaagaagg ggaacaacgc ctccttagat tgcttttgca
     6481 gaacggtgcg gatcctgaat ggatacaacg atatactgga ctttcggcag agcaaatgca
     6541 ggcattagag caacccttgc ctgaaagcaa gcgcgatcca tggatcgagt actaatcaga
     6601 gactgatgac aaacgcaaag ctgcctgatg tgctacgctt atcagaccta cattacctct
     6661 gcaatctatt gaatttgcgc gatttgtaag ccggataagg cgttcacgcc gcatccggta
     6721 tgaacaaagc acactttgcc taagccatgc tggaacgtgg cgatgcccct gcaaagataa
     6781 aacgagtcac aggcgattcg tgagaggaaa tcaggggcct cgccactaat tcgaattcat
     6841 tccattgcct gatacacggc ctcgccaatt tgcttcagtg cttcgcgata ggtttcgctg
     6901 agcggcaaag cgcagttgat acgtagacaa ttacggtatt tgccggaagc cgagaaaatc
     6961 gagcctgccg ccacctggat tttcatgcgg tatagctgcc gcgcgacgca gaccatatcg
     7021 acctgttcag gcaattcgat ccacagtaaa aatccgcctt tcgggcgcgt aatacagatt
     7081 tcgcagggaa aatattcccg tatccagcag gtataaagcg ccaaattgcg ctgatagatc
     7141 tgccgcatcc gccggatatg gcgatgatag tgaccttcca gcacaaacgt cgccgccgcc
     7201 atttgcgtag acggtacatt aaagctgctg atggcgtatt tcatatgcag cagtttatcg
     7261 tgataacgcc ccggtgcgac ccagcccacg cgcaggccag gtgcaatact tttactgaac
     7321 gagctgcaca acagcactcg cccgtcgata tcccatgaat gaatggtccg cgggcgcgga
     7381 tactccgttg ccagttcgcc gtagacatca tcttcaaaaa tcacaatatc atgacgctga
     7441 gcgagagaga gaacggcccg tttgcgcgcg tccggcataa taaatcccag cggattatta
     7501 cagtttggca ccagaatgat gcctttaatc ggccactgtt ccagcgccag ttccagcgct
     7561 tcaacgctga tgccagtttc tggatcggtt gggatttcaa tcactttcac gcccatgccg
     7621 cgcagcatct gcatcgaacc gtaataacag ggagattcga ccgcgacaat atcgcccggt
     7681 ttacacaccg ccattaacgc cagcgacatc gagttatggc agccgctggt gatgatgatg
     7741 tcatcggcgg tgaccaccga gccgctgtcg agcatcaggc gggcaatctg ctctcgcaat
     7801 acccgctgac cggctaacaa gtcataaccg agaacggttt gcagattatg ctgcaccacc
     7861 cggcttagct cacgccacaa cggtttcagg ctgggcgttt cgacatccgg cgtgcttttg
     7921 cttaacggaa caatggaact gtcgctatgc gcctccagca tatccagcac ctgatcccac
     7981 tgggtaattt ccaccgggcg ctgcaccgga cgcgtcatcg gcggcactgg cggctgggct
     8041 ttacgttgtg cgacaaaata ccccgaacgc ggctgcggag tgatgagctt catcgtctcc
     8101 agcgtctgat acgcctgctg cacggtactg atgctgacgc cgtgctcctg acttaagctg
     8161 cgcaccgacg gcaatttctc cccgtgacga tacagcccct gctcaatccg ctcggcaagt
     8221 agagtcgcca gatgttgata acgcgtcatg ctgtattcct tatttagacc atacagagag
     8281 aaaaaccggt acagatgagg ctaaaaaacc ggatgcagat gctgttttaa cctgtctgta
     8341 tgttaaataa aactaatatt tgcatctgta ttgtgaactg ccagaaggcg gatgatgaag
     8401 cctctgaaag atgaggaggc agcagatgga atttcacaaa aacaaagcca aaacgccgtt
     8461 tatcagactt gtgctactct ggcatgcgat gaggcgttgg cggcggcaaa tgcagacccg
     8521 acgagtgtta cagcagatga gtgatgagcg gttgagggat attgggttac gcagggagga
     8581 tgtggagtga gggggatata aatttatata ataaaacatt tttatgtttt taaattaagt
     8641 tataaaaatt ttcccgagac aattcatca
//
LOCUS       DAZGBC010000183         8598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGBC010000183 DAZGBC010000000
VERSION     DAZGBC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.184"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHK1J_004302"
     CDS             <1..573
                     /locus_tag="ACHK1J_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO7913493.1"
                     /translation="YIHPDEDNKFDNFMRQNRFSKVNFDTQQMIDFINRFNMNKWLID
                     RNNNFFIQLIDQALRSTNDTIKENAWHLYKEWIRSDDVSPLFIEIEDNLRTFNTNELT
                     RNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEILPVNLTPET
                     LFGSKSQDKHALFPIFTASWRANRIKNKGI"
     gene            796..1293
                     /gene="ubiC"
                     /locus_tag="ACHK1J_004303"
     CDS             796..1293
                     /gene="ubiC"
                     /locus_tag="ACHK1J_004303"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO7913494.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1306..2178
                     /gene="ubiA"
                     /locus_tag="ACHK1J_004304"
     CDS             1306..2178
                     /gene="ubiA"
                     /locus_tag="ACHK1J_004304"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO7913495.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2333..4756)
                     /gene="plsB"
                     /locus_tag="ACHK1J_004305"
     CDS             complement(2333..4756)
                     /gene="plsB"
                     /locus_tag="ACHK1J_004305"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO7913496.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPTHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4927..5295
                     /gene="dgkA"
                     /locus_tag="ACHK1J_004306"
     CDS             4927..5295
                     /gene="dgkA"
                     /locus_tag="ACHK1J_004306"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO7913497.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5405..6013
                     /gene="lexA"
                     /locus_tag="ACHK1J_004307"
     CDS             5405..6013
                     /gene="lexA"
                     /locus_tag="ACHK1J_004307"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO7913498.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6086..7411
                     /gene="dinF"
                     /locus_tag="ACHK1J_004308"
     CDS             6086..7411
                     /gene="dinF"
                     /locus_tag="ACHK1J_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO7913499.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLFGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFSAT"
     gene            7527..7736
                     /gene="yjbJ"
                     /locus_tag="ACHK1J_004309"
     CDS             7527..7736
                     /gene="yjbJ"
                     /locus_tag="ACHK1J_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO7913500.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7778..8293)
                     /gene="zur"
                     /locus_tag="ACHK1J_004310"
     CDS             complement(7778..8293)
                     /gene="zur"
                     /locus_tag="ACHK1J_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO7913501.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2001 a         2069 c         2301 g         2227 t
ORIGIN      
        1 tatatccatc ctgatgaaga taacaagttc gataatttta tgcgtcaaaa tcgattcagt
       61 aaggtgaatt tcgataccca acagatgatc gattttatta acagatttaa tatgaataaa
      121 tggctgattg atcgaaataa caattttttt atccaactta tcgatcaggc tctacgatca
      181 acgaatgaca cgatcaaaga aaatgcctgg catctttata aagagtggat tcgtagtgat
      241 gatgtttcac ctttatttat agaaattgaa gataatttaa gaacctttaa cacgaatgaa
      301 ttaacacgaa acgataatat ctttatcttg ttctcctctg tcgatgatgg gccagttatg
      361 gtggtaagct cccagcgctt acatgatatg ttgaatccta caaaagatac caattggaat
      421 tccacgtata tctacaaatc cagacatgag atattgcctg ttaatcttac tccggaaaca
      481 cttttcggct ccaaatctca ggataaacat gcacttttcc ccatttttac tgcgagttgg
      541 cgagctaatc gtataaagaa taaaggtatt taagtaaagg aaaacatcac cgttcctggc
      601 atcctgggcg gtgatgcccc tatggttgcc ctcgccagca cgggcatcgg caaagcgtaa
      661 ggttcaacag cgttttacca cttcatgcga ttgttgcgtt tttgttgcgc attagatcac
      721 ttaatttgct ttacatctcc cgtaaacact tttctgcgat acaatgcctt tacgttatgt
      781 aacggagagt tcggcatgtc acaccccgcg ttaacgcaac tgcgtgcgct gcgctatttt
      841 aaagagatcc ctgccctgga gccgcaactg ctcgactggc tgttgctgga agattccatg
      901 acaaaacgtt ttgaacagca gggaaaaacg gtgagcgtga cgatgatccg cgaagggttt
      961 gtcgagcaga atgaaattcc cgaagaactg ccgctgctgc cgaaagagtc tcgttactgg
     1021 ttacgtgaaa ttttgttatg tgccgatggt gaaccgtggc ttgccggtcg taccgtcgtt
     1081 cctgtgtcaa cgttaagcgg gccggagctg gcgttacaaa aattgggtaa aacgccgtta
     1141 ggacgctatc tgttcacatc atcgacatta acccgggact ttattgagat aggccgtgat
     1201 gccggactgt gggggcgacg ttcccgcctg cgattaagcg gtaaaccgct gttgctaaca
     1261 gaactgtttt taccggcgtc accgttgtac taagaggaaa aaaatatgga gtggagtctg
     1321 acgcagaata agctgctggc gtttcatcgc ttaatgcgta cggataagcc aattggcgcg
     1381 ttactgctgc tctggccaac attatgggcg ttgtgggtgg cgacaccggg cgttccccag
     1441 ctctggatcc tggcggtgtt tgtcgcgggt gtctggctga tgcgcgctgc cggatgtgtg
     1501 gtgaatgatt atgctgaccg caagtttgat ggtcatgtta agcgcacggc gaaccgacca
     1561 cttcccagcg gcgcggtaac agagaaagag gcgcgcgcgc tgtttgtagt gctggtactg
     1621 atttcgtttt tactggtgct gacgctgaat acgatgacca ttctgttgtc gattgccgcg
     1681 ctagcgctgg cgtgggtgta cccgtttatg aagcggtata cccatctacc gcaagtggtg
     1741 ctgggcgcgg cgtttggctg gtcgattcca atggcttttg ctgctgtgag tgagtcggtg
     1801 ccattgagtt gctggttaat gttcctcgcc aatattctct gggcggtggc ttacgacacg
     1861 cagtatgcga tggttgaccg tgatgatgat gtgaagattg gcattaaatc cacggcaatc
     1921 ctgttcggcc aatacgataa attgattatt ggtattttgc agattggcgt actggcactg
     1981 atggcgatca tcggtgagtt aaatggctta ggctggggat attactggtc aattctggtg
     2041 gctggcgcgc tgtttgttta tcaacaaaaa ctgattgcca accgcgagcg tgaagcctgc
     2101 tttaaagcat ttatgaataa taactatgtt ggtctggtac tgtttttagg gctggcaatg
     2161 agttactggc atttctgatg atgtaaaaaa gccggatgat catccggctt tcttctgggt
     2221 tgcctgatgc gctgcgcttc tcaggcctac acaacagatc gcaatttatt gaatttgcag
     2281 attatggaag gccggataag gcgtttgcgc cgcatccggc aattctctct gattaccctt
     2341 cgccctgcgt tgcactctca atcgtcaaac gcacgtctga tgtaatcaac tccgccagca
     2401 actgataaac cttcatcgtt tctgccggtt cggcatcgcc gctatcgctg atataccctt
     2461 catcacgtag cgtcagcacc agagaactga acaccgcctt gtcgaagaac tccggcgcgt
     2521 tgataccgtg cagcacggag agacgttgcg cgacggtgcg gctctctttc tccagcgtac
     2581 cgcggttgat cgacgggttg gcgctcaaca gccagaaggt gatagcataa cgttgcagcg
     2641 tttcgcgcgc gcctgcggcc agcagctgta gcgtgcgaga atgcgtcggg ttgatatgca
     2701 actcatcatc ttgcagggta atcagcccct gacgttgcat ctcatttgcc agcgcatcaa
     2761 taacgtccgg caactcgtcg cgatcccagc gcaggaacag ctccgctttc agcattgggt
     2821 aaagcacatt gacgtgctcc atcaatacgt cgcgggagat gtggcgatgc tgggtgacaa
     2881 ttgccgccat cagcgaaggc agcaccaaca tatgcgcaat gttgttgcga tagtaggtca
     2941 tcagcaccgc ttgctcgcgc ggcagaatga tgatgtcgcc gatagtgtct ttctcaactt
     3001 caaacttgtt catttgcagc gcgtgatcga taagctcgct ggcgctggct gaaggaacgg
     3061 tagagtccgt ggagtaaggc acgttgcgca tcagatccag gtagcagttg agttgctcgg
     3121 ttaactgctc gcgggtgagt gagcgctgac gtgatgccag tagcgcagta cagcacaggt
     3181 tcatggcgtt tgccgcgcct gcgttgttaa tgcgtaccat cagatcggca gcaatattat
     3241 tgaccgtcgg tgttaaccat gccggacgca ccgcttcgat gggatcgata gattcacgcc
     3301 agtcaggtac gtgctggtta aggtaggtca tcaacggcat tggttcaccg aaattgacgt
     3361 aaccctgacc gagattacgc agcttgctta aaccgcgcag catctgcggc aggctctctt
     3421 tctctttcgt cgcgccgcgc agttctttgg cgtaagtacc cacttccatg acgtgctcat
     3481 aaccgatata gatcggaatc agcgtaatcg gacgcgtgcc gccacgcagc atcgcctgaa
     3541 tggtcatcga cagcgtaccg gttttcggat ccagcaaacg ccccgtacgg gaacgaccgc
     3601 cttctacgaa gtactcgacg gaataaccac ggctgaacag ttcgccgaga tactcacgga
     3661 aaacggtgga atagagctta ttgcctttaa aagtacggcg aataaagaac gcacccagac
     3721 ggcggaaaat cggcccggcc ggccagaagt tcaggttgat cccggcggcg atatgcggcg
     3781 gcaccagccc ctggtgatac agcacgtaag aaagcagcag gtagtccatg tgactgcggt
     3841 ggcaaggcac atacaccagc tcatgaccgt cgtgggccaa ctggcgaacg cgctcggcgt
     3901 tatggacgtt gataccctgg taaagtcggt tccaggtgaa gcccagaata cggtcagtca
     3961 ggcgaatcat ctcgtaagag aaattcgccg caatctcttc catcagtgca atcgcgttct
     4021 gctgcgcttt ttcatgggag atttttttgc tgcgcgcttc atcttctacc gctttcgcaa
     4081 tggcgcggga ggcgagcagc ttattaaaca gatcctgacg agcaggcaaa cgtgggccta
     4141 cggcagccag acgttgacgg gcaaagtgca tacgcgccac gcgcgccagt ttctgcgcga
     4201 tagttttatc cgtgccgtgt tcatccgcca tacggcgcag cgaaacggac ggtgagaaac
     4261 gcacaaaact gtcgcgaccg agccacagta cagcgaaaaa tttctgtacg ccgttaagca
     4321 tacgcagcgg cgggttcact tcgccttttt cacgccccgg cgcgcgacca aacatcaccg
     4381 acactggcac catctgcaca tccagatttg ggttgctacg gtgcaaatcg agatagtcgt
     4441 ggaacagctt aatagactct tctttcggcg tgtaataggt gaatacacgc ggcccgccgt
     4501 gaatgaacac atagcgcggc agtagcgtgc cgtcgatttc cagcggctct aacgggtcag
     4561 gcaagtcatg tgccagacac tgggcgcgca acgtcagcaa gtctgctttc gagttgtacg
     4621 gtaaaacgta cataattgga cgagaggtat ccagccccag ttccggggca ggatctgccg
     4681 gaatagactt gctttttacc aggatgctta atggtaaatt cagtaatttg tagtaaattc
     4741 gtggccagcc ggacataaac gatgtaaagc ctctggttaa taatgcaaat gcgcggcaag
     4801 gatagcagaa agtcatggga aattctgtgg tatccgctca tgtttcgcgc ggcgttacgc
     4861 aaacccgaat catcggattt aacggtacac tgatattgac gctcataatg taaaaaggtt
     4921 ctttcaatgg ccaataatac cactggattc acccgaataa tcaaagctgc tggctattcc
     4981 tggaaaggtt tacgcgctgc atggatcaac gaagcggcat tccgtcagga aggcgtagcg
     5041 gtattgttgg cggtggtcat cgcctgctgg ctggatgtgg acgcgattac ccgcgtgctg
     5101 cttatcagct ccgtgatgct ggtgatgatt gtggaaatcc tcaatagcgc catcgaagca
     5161 gtggttgacc gaattggctc tgaataccat gagctttccg gacgcgctaa agatatggga
     5221 tccgccgcgg tgctgattgc gattatcgtc gccgtgatta cctggtgcat cctgttatgg
     5281 tcgcattttg gataaccctt ccagaattcg ataaatctct ggtttattgt gcagtttatg
     5341 gttccaaaat cgccttttgc tgtatatact cacagcataa ctgtatatac acccaggggg
     5401 cggaatgaaa gcgttaacgg ccaggcaaca agaggtgttt gatctcatcc gtgatcacat
     5461 cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg cagcgtttgg ggttccgttc
     5521 cccaaacgcg gctgaagaac atctgaaggc gctggcacgc aaaggcgtaa ttgaaattgt
     5581 ttccggcgca tcacgcggga ttcgtctgtt gcaggaagag gaagaagggt tgccactggt
     5641 aggtcgtgtg gctgccggtg aaccgcttct ggcgcagcag catattgaag gtcattatca
     5701 ggtcgatcct tccttgttca agccgaatgc tgatttcctg ctgcgcgtca gcgggatgtc
     5761 gatgaaagat atcggcatta tggatggcga cttgctggca gtgcataaaa ctcaggatgt
     5821 acgtaacggt caggtcgttg tcgcacgtat tgatgacgaa gttaccgtta agcgcctgaa
     5881 aaaacagggc aataaagtcg aactgttgcc agaaaatagc gagtttaaac caattgtcgt
     5941 tgaccttcgt cagcagagct tcaccattga agggctggcg gttggcgtta ttcgcaacgg
     6001 cgactggctg taacatatct ctgagaccgc gatgccgcct ggcgtcgcgg tttgtttttc
     6061 atctctcttc atcaggcttg tctgcatggc attcctcact tcatctgata aagcactttg
     6121 gcatctcgcc ttacccatga ttttctccaa tatcaccgtt ccgttgctgg gactggtcga
     6181 tacggcggtt attggtcatc ttgatagccc ggtttatttg ggcggcgtgg cggttggtgc
     6241 aacggcaacc agctttctct ttatgctgtt gctgttttta cgcatgagca ccaccgggct
     6301 gactgcgcag gcttatggtg ccaaaaatcc tcaggcatta gcccgtgcgc tggtgcaacc
     6361 gttgctgttg gcgttggggg ctggggcgtt aattgcgctg ctgcgtacac cgattatcga
     6421 tctggcgctg catattgttg gcggtagtga agcagtcctg gaacaggcgc ggcgctttct
     6481 tgaaatccgc tggttaagcg caccggcgtc gttggcgaat ctggtattac tcggttggtt
     6541 actcggcgtg caatatgccc gtgcgccagt aattttgtta gtggtcggca atatcctcaa
     6601 cattgtgctg gatgtctggc tggtaatggg gctgcatatg aacgtgcagg gtgcggcgct
     6661 ggcgacggtt attgcggaat atgcaacatt gctgtttggt ctgctaatgg tgcgtaaaat
     6721 cctcaaacta cgcggaattt ccggcgaaat gctgaaaact gcctggcgag gaaacttccg
     6781 tcgcttgctg gcgcttaacc gcgatatcat gctgcgttcg ctgttgttgc aactctgttt
     6841 cggcgcgatc accgtacttg gtgcgcgact ggggagtgac attatcgctg ttaacgcggt
     6901 tctgatgacg ctactcacct ttaccgccta tgcgctggat ggttttgcct acgcggttga
     6961 agcacattcc ggtcaggcat acggtgcgcg cgacggtagc cagttgctgg atgtctggcg
     7021 ggcagcgtgc cgccagtcgg ggatcgtagc gttactgttt tcggtggttt atttgctggc
     7081 aggggaacac atcattgcgt tgctgacgtc gttaacccag attcagcagc tggccgaccg
     7141 ctatcttatc tggcaggtga ttttgccgtt ggttggcgtc tggtgttatc tgctggacgg
     7201 catgtttata ggcgcaacgc gtgccgccga aatgcgtaac agtatggcgg tggccgccgc
     7261 aggttttgcg ctgacgctcc ttacgctgcc gtggctgggc aatcatggtt tgtggctggc
     7321 gttaaccgtc tttctggcgt tgcgcgggct ttctctggcg gctatctggc ggcgtcactg
     7381 gcgcaatggt acctggtttt ccgcaacgtg acggttaaaa attctgaata aataatccta
     7441 agccaaattg ctgactacac ttaatctcac gttcagaaga aaagtgaacg tactctcatt
     7501 cacaacctaa cgatgaggtc ttgattatga ataaagatga agccggcggt aactggaaac
     7561 agtttaaagg taaagtgaaa gagcaatggg gcaaactgac cgatgatgat atgacgatca
     7621 ttgaaggtaa acgtgatcaa ctggtcggta aaatccagga acgttatggt tatcagaaag
     7681 atcaggcaga aaaagaggtc gtggattggg aaacccgcaa tgaatatcgc tggtaattaa
     7741 tccctcctgc ccgacgtgta caaggatgta cgccctctta acgcggtttc tttttcacct
     7801 gcacagagtg atcatgctgg cactgttcag gatgacgaca cgcttccact tccacgcatg
     7861 ccgcacagag cccatgtgct tcaatcacat tatgccgcag ggcaaacccc atttttgccg
     7921 ccagcgtatg catgatgtct tccacgcctt ctgcacattc ttctttcacc gcgccgcatc
     7981 gatcgcaaat aaacatagct gacgtatggg tgggctggtc gaacagatga cagagcacat
     8041 aactgttggt ggattccacc ttatgcacaa aaccttgctc aagcagaaaa tccagcgcgc
     8101 gataaaccgt tggcggcttg gcttgcggtt cagcttcgcg cagcaaatca agcagatcgt
     8161 aagcgctgat agcgccatcc tgcagactca tcaggcgcaa cacttccagg cgctgtgggg
     8221 tcaggcgcac attacgctgc gcgcagattt tttcagcctg cgctaataac tcctgcgttg
     8281 tggtcttttc catcgggcac ctcaaagtgg gggttaagaa tgtccttact ttaccatgtt
     8341 ccaggaaaaa caccgagaac ccttattgtt gtcgtaatgt tgattttctg ttttgtacgt
     8401 aaggtgttgt tttgcctggt cgtaccatta tcaacacgat aataattaat aattcatttt
     8461 taaataaatt gcttcaagtt aacttgctat ttatttaacc gtggctaatc gtatacccga
     8521 atttcagaaa attatgagcg atttttgata gtgaccggtt ccaaaaacac ttataaggag
     8581 gacacggtac gacaaaaa
//
LOCUS       DAZGBC010000184         8497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGBC010000184 DAZGBC010000000
VERSION     DAZGBC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.185"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHK1J_004311"
     CDS             <1..129
                     /locus_tag="ACHK1J_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7913502.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHSRYVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHK1J_004312"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHK1J_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7913503.1"
                     /translation="MERESQPVRTRLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDHPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHK1J_004313"
     CDS             1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHK1J_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7913504.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINADGAQTPLATMVRVYQLRDRKAVDAADYQTLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            1744..4266
                     /locus_tag="ACHK1J_004314"
     CDS             1744..4266
                     /locus_tag="ACHK1J_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7913505.1"
                     /translation="MDNRSAFFDGQTRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LEYTRQCAVFQNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFI
                     QRILSEVGIYWRTEMDDVRGLDTYIFADSQLHYQFDVQLPYSEPSGLFDGAAESVWDV
                     RTWHNVATGTVTTRDYNYRTATTPMNATVSVRNDAVTTGEHYRYAAPYREAGDDASPE
                     PETESGAFYARLHHERELNRSACIHLFSNAAHLSPGQVLEPQGDVITDLKEGIVLTLV
                     TYRGARDSRLHVSAWGMPYTERYCFRPAEIPRPVIPGTLPARIESRGKNDIYAHLDGQ
                     GRYRVKMDFDREGTEPGFGYLWLRMAKPCAGETLGWHMPLIDGTEVAIAYSNGDIDLP
                     YIAYALHDAEHPDLVNRDNHTRNILRTPANNKLRMEDRRNEEHIKLATEYGKTQLNSG
                     HLVDSQGQLRGKGTELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLN
                     QQLQQLENAAERAQALKADIDSQIQMFEQRLKPLNEALVFSAPEGMALTSGEDMQMSA
                     ARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGPVEVQAQNGSMRLF
                     AEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKVEYGTTASYLRKVKRTMTAAAQ
                     SMSMAFPRMSTSHIYSAIYRLLDKHGNILVNIPYRLTTPSGQTVTGYSDNEGRSVPVY
                     TRQQEDVELHFFVKKPQPEETMWFVGETDTQQLETELRESLP"
     gene            4266..5555
                     /locus_tag="ACHK1J_004315"
     CDS             4266..5555
                     /locus_tag="ACHK1J_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO7913506.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGGIGHEDRMTILEIHDCRP
                     EEHQWVDQVINVTMKAWQTSGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKAVS
                     DLSEGVVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVK
                     SWTNTQIKAAWTEIQHATDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLG
                     IPEAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVG"
     gene            5568..6782
                     /locus_tag="ACHK1J_004316"
     CDS             5568..6782
                     /locus_tag="ACHK1J_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFO7913507.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNPGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGGAVIGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIKPVRFNESRSLHFYSEYEGLQFDKESTMKWYRRFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKYGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            6808..7187
                     /locus_tag="ACHK1J_004317"
                     /pseudo
     CDS             6808..7187
                     /locus_tag="ACHK1J_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
     gene            7184..7687
                     /locus_tag="ACHK1J_004318"
     CDS             7184..7687
                     /locus_tag="ACHK1J_004318"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO7913508.1"
                     /translation="MNSVSFSLAAQHLICSTKVTLFALVDGLQYERYFGESLSVQQPA
                     AVPLFDTWPDSRIAFAGPWVMEMNSIMDFRERLCELEAALPSVSWMISSSSLTELAAH
                     FRRNMNTELPDGRIALLRFHDPRVQKRLGEMLNDQQHRELTGLMQEWLTIVDGKAWSF
                     KQREFIC"
     gene            7681..8025
                     /locus_tag="ACHK1J_004319"
     CDS             7681..8025
                     /locus_tag="ACHK1J_004319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913509.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVCDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            8025..>8497
                     /locus_tag="ACHK1J_004320"
     CDS             8025..>8497
                     /locus_tag="ACHK1J_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913510.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQ"
BASE COUNT         2059 a         2062 c         2547 g         1829 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctat gctgatgttc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacag ccggtacgta
      121 cccggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcagaccct ggcccgggat gggattcccg gtcagcaccc
      301 tgaaagcggt ggagatcgac gaagatcatc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgccgtga aggacatgag gcactcacct catttctgga tattttcagc catcgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtaccc ggccactttt gaggaggggg
      541 ggcgcgatgc cacatcgcag tgtctgctgg ggctggtcgg gctcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt tcctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgcggaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac cgaacccgat ccggttaagg tccatatcgg taaccgcagt ggactgggtg
      781 ccggaaaccg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgaa cgaagcggaa ggctggttgc
      901 ccggtggtgt cctccatacc gatttgctgg tcctgctgcg tgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgcctt actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttgctggg gctgaaagcg ggcaaactca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggctgcta cgaagggctg aaatgtgctg
     1141 cgctaccgcc tgctgaagac ggacattatc gcttcgaata atcacctgtt ctctctcaat
     1201 aaaggaacgc ctgaatggta acagtaatcc gctggtgggc gccgatgttt attgcctgtg
     1261 gcctgctcgc aggctgcggt ctgacgcaga ccgtcacgga cggcacgatc agcatcacga
     1321 aatccatctt ctataaaaaa atcaaaacgc tgcatctgga ttttaccccg cgtacggcca
     1381 ttaacgctga tggtgcacag acaccactgg cgacgatggt gcgggtttac cagctcaggg
     1441 atcgtaaagc ggttgatgcc gccgattacc agacactgct gcgtaatgct gatacggtac
     1501 ttaaggacga cgtgctggcc tcaaaagaac tgctggtgat gcccggcggc agcgtgacgc
     1561 ttaatatgcc catggatgaa gatgcgcagt ttgtggctgt ggtgggactg tttaaccggc
     1621 cggatcagaa ggacaatcgc tggcgcctgg tactgacccg tgatgacctc aatccggata
     1681 agccccgcac cattgaactg ggtgatggct ggctcagcct tgtgccggta aaggagtgac
     1741 gtgatggata accggtcagc gttttttgac gggcagaccc gttattttct ggaaattaat
     1801 ggaagtgcag taaagcctga tgttttacgt tttcgtggca gggaagcact gagtgagccg
     1861 ttccgatggg acatcgggtt cacctcccgg aatgcgaaca ttcctccgga agaggtgctg
     1921 atgaaatatg cgtcgttgcg gatgcgtaac ggcaaaaatg tccatggcat tgtgacccgt
     1981 ctggaatggc tgtccacatc gaaagaccag tcccactacc ggcttacact cagttcccgt
     2041 ctggcactgc tggagtacac ccggcagtgc gccgtatttc agaaccagtc cgtccctgag
     2101 gtggtggagc aggtgctgcg caaacacggg ctggagggcg ctgattttga attcagactg
     2161 gaacgcgctt acccgtcgcg tgaaattatc acccagtggc aggaaacgga tttgcagttc
     2221 attcagcgca ttctgtctga ggtggggatt tactggcgca cggagatgga tgatgtgcgc
     2281 ggactggata cgtacatttt tgccgacagc cagcttcact atcagtttga tgtgcagttg
     2341 ccttacagcg agccctcggg gttgttcgac ggcgcagcag aatcggtctg ggatgtgcgg
     2401 acctggcaca acgtcgccac cggcactgtc accacgcgtg attacaacta ccggacagcc
     2461 accacgccga tgaatgcgac ggtcagcgtg cgtaatgatg cggtcaccac cggggagcat
     2521 taccgttacg cagcacctta tcgtgaagcg ggagacgatg ccagcccgga gccggaaacc
     2581 gaatctggtg cgttttacgc ccggcttcac cacgagcggg aactcaacag gtcggcctgc
     2641 attcacctgt tcagcaacgc cgcacacctc tctcccgggc aggtgctgga gccgcagggg
     2701 gatgtcatta cggacctgaa ggaagggatt gtcctcacac tggtgactta ccggggagcc
     2761 cgggattccc ggctgcatgt gtcagcatgg ggaatgcctt acaccgaacg ttactgtttc
     2821 cgtccggcag aaatcccgcg tccggtcatc ccgggtacgc ttccggcacg gattgagagt
     2881 cgcgggaaga acgatatcta tgcccatctg gacgggcagg ggcgttaccg ggtgaagatg
     2941 gactttgacc gggaggggac ggaacccgga ttcggctacc tgtggctgcg gatggcaaaa
     3001 ccctgtgccg gtgaaacgct gggctggcat atgccgctca ttgatggtac cgaagtggcg
     3061 attgcataca gcaatggcga tattgacctg ccgtacatcg cgtatgcact gcacgacgct
     3121 gagcatcctg acctcgtgaa ccgggacaat cacacccgga acatcctcag aacgcctgcg
     3181 aacaacaagc tgcggatgga agacagacgc aatgaggagc atatcaaact cgccacggag
     3241 tacggtaaga cacagcttaa cagcggacat ctggtggaca gccaggggca actgcggggt
     3301 aaaggcacag agctgcgtac cgatgagtgg ggaaccctga gagcggggaa ggggcttttt
     3361 gttgctgcgg atgcgcaggc gaaagcacag ggcgaggcac tggacatgga tgccgcgctg
     3421 aaggaaatcg acaggcttaa tcagcaactg cagcaactgg aaaatgccgc agaacgggca
     3481 caggcgctga aggctgatat tgacagtcag atacagatgt ttgagcagcg gctgaaaccg
     3541 ctcaacgaag cgctggtctt ctccgctccg gaaggaatgg cgctgaccag cggtgaagat
     3601 atgcagatga gtgcagcacg aaatgtggct gtcaacgccg gaggtgatat cagtaccgga
     3661 acaatgggca acctgaccgc gctggccgga gagaagctgg ggctgtttgc ccggaccggt
     3721 caacttagcc tgaaatccgg ggaagggccg gtagaggtac aggcgcaaaa tggcagcatg
     3781 cggttgtttg ctgagaagaa gctgacactg agctcagcga gtgatatctc ctttgccggg
     3841 aaaaaacgca tcacgctgat tggcggggga agttatctgc gacttgaggc cgggaaggtt
     3901 gagtatggta cgacggcgtc gtatctgcgc aaagtgaagc ggacgatgac ggcggctgcg
     3961 cagagtatgt cgatggcatt cccccgtatg agtacttcgc atatctactc ggctatctat
     4021 cggttgctgg acaaacacgg caatattctg gtcaatatcc cctatcgcct taccactcca
     4081 tctggtcaga cagtaacggg gtatagcgat aacgaaggcc gctccgtgcc ggtttatacc
     4141 cgccaacagg aggatgtgga actgcatttt tttgttaaaa aaccacaacc cgaagaaacg
     4201 atgtggtttg tgggggaaac cgatacgcag caactggaaa ccgaactcag ggagagcctc
     4261 ccataatgga aatgcccgat ttacccgaga caccgacatc tgatattccc accggggcga
     4321 acaaccagcc gtgggattgc accacgacgg gtgtcgaaat ggtcgaaaag cagtcgcgtc
     4381 tgccaaccac ggaaaaccgt cagtacctgc atataaaggt ggaatacgcc atgcgttttc
     4441 cgcaactgtc ggcacaaatc cgccggaaag gtaaaactta ccagatgagc cttaagcaga
     4501 ccgttatgag tggtcttatt cgcgtggatg aaatcgcccg caattactgc tggtattata
     4561 aggcggaggt ggcgtttgat atgcgcacca cgccaccacg cccttttctg agcagtacgc
     4621 tgctggaaaa agacggtgct ggccgtcgcc acacgaccaa tcctttcccg gtgcccggca
     4681 ggggcattta ccgccgcccg gacgtgatta ttgttaaaaa taaggaggac cgctggcccg
     4741 gactggcggg gccggatacc gaaggcaata tgcacagcga taacctggcc cgcctggtgg
     4801 aggtgaagtt tccgggagat aggctgtatg aagaccaata tgaagagtat atgcagattg
     4861 tcggtggaat tggtcatgag gatcgcatga ccattctgga gatccacgac tgtcgcccgg
     4921 aagagcacca gtgggttgat caggtgatca acgtgacgat gaaagcctgg cagaccagcg
     4981 gcgcgaaata ctggccgctg gctctctacc cgccggtctt tatgccccgt ccgccatcaa
     5041 cccccgttgc tgccagaata gagccctgga cttacgccgg taaagcagta tctgatctca
     5101 gtgagggtgt ggtacgtggt gtggctgaag gctggaatgc cctgtcaaaa gagacgcaac
     5161 tggtcctgaa ttcagcagcc agctggctta acgacagcgg tgagtgggtt taccggcagg
     5221 gaagtgaggt ctgggtctgg gccagccaaa ccggtaagtc ggtgaaatcc tggaccaaca
     5281 cccagattaa ggccgcatgg acagaaatac agcacgccac cgatatgacg cttgatatgc
     5341 tgaaacaggt ggactgggta caggttttaa ccagtgtcgc gaaaacggtc ggcgttattc
     5401 tggttgctgt gggtgtcggc ggactggtcg taacgctggg tatcccggaa gccattattg
     5461 gcgcgttttt actgattgtt cgtctggcaa taagcgcctg gggaacactg gcgacgattc
     5521 tcggtacggg tacggcggta ctggcagttg gttaaacagg agcaggtatg gaattgcatg
     5581 attttgagct gaactacatt gagaaatggc tgccggaagc ctccgtgaaa tacaaagacg
     5641 gcagtccggc agtcaatccc ggtctgatta tcaccgtctt ttttaaggac ggacatacgc
     5701 cagaagtccg ccgcaggatg gtggagtgcg tggatcgctt ctatggggag tttaaaccct
     5761 ggctgaaaaa aacgatcacc aaaaactggg ttggcatcac tgataataac tacgccaaaa
     5821 agcggcagga gatcctcgat tcaacgcctg aagagatttt ttcctggtat ctgagtagcg
     5881 cagagaagga ttttttagcc cccgattaca gtattttaat tatggggaaa agaatttttc
     5941 ataatgacaa taatcgttca gtcattaagt taacactccc gttgtcgctg ctgaaagaaa
     6001 ctgatggaaa gcagcgttat gaagcctggc tgatgtggct gtgtgacacc ttttcggtgg
     6061 aaagtggtta tgcagggttg tcgtttgcac ttccttatgc ccgtgagcga atgttcccct
     6121 gtgaattcgc cctggcgcag cgtttttccg gtgtgatggt ggattcactg ggaacgctgg
     6181 aaggcggtgg ggcagtcatt ggtctgaaag gtgcctgctg gtacaccatt ctcggtacgc
     6241 cgtggctgga aaaactgggg ggggcagaga gagtaaaaca tcgtttagct aaaacgcctg
     6301 aaatcagcct gctaccctat aacaatggtg tcgtacttaa agccggtgaa ctccccccgc
     6361 cactgggaga gatgaaaaca gaaggtttat cgccgctgct ggtgaaggtg aaccaggtca
     6421 ttaagccagt gcggtttaat gaatcaagat ctcttcattt ctactctgaa tatgaaggac
     6481 ttcaattcga caaagaatcc accatgaagt ggtacaggcg ctttgacgaa gccagcgccc
     6541 tgctcgataa agaagaaatg gggaaatctt gcgcccccga acgcattaaa tgctggaccg
     6601 atgaaatagc gccccatgcc ggacaatggg ccgcgatggt taatggcacc accgagtaca
     6661 tccagacccg cgaagggcag aaaatgcccc cgtttgagga taagtacggt aagcagcaca
     6721 gggcttgctg gaaactactg aaacgagatg acaatggtag cgtttataca ctacctgagt
     6781 aaggtcgtgt gctgaatgac cgcgcaggcc ggagagaggc tggggctttt tgcccggacc
     6841 ggacaactga gcgtcaagtc cggggagggg ccggtagagg ttcaggcgca aaacggcagc
     6901 atgcggttgt ttgctgagaa gaagctgaca ctgagctcag cgtgtgatat ctcttttgcc
     6961 gggaaaaaac gtatcacgct gattggcggg ggagttacct gcgacttgaa gccgggaagg
     7021 tcgagtatgg tacgacggca aagtatctgc gcaaagtgaa gcggacaatg gcggcggggg
     7081 ctgcatcaat accagtcagg tccattgtgg ggggaggtat ttgcctgagc tgtctgatga
     7141 aggcggcaat gaatggcgac acctttgttg taaggggtgg gtcatgaaca gcgtcagttt
     7201 tagccttgcg gcacaacacc tgatttgttc aacaaaagtg acattatttg ctctggtgga
     7261 tggtctgcaa tatgagcggt atttcggtga gagtctgtct gttcaacaac ctgccgccgt
     7321 gcccctgttc gatacctggc ctgattcgcg tattgcattt gcggggccat gggttatgga
     7381 gatgaacagc ataatggatt tcagagaaag gctgtgtgag cttgaagctg cgcttccgtc
     7441 agtttcctgg atgatatctt cttcctcgct tactgaactg gctgctcatt tcagaaggaa
     7501 tatgaataca gaactaccgg atggacggat tgcgttgctg cgtttccatg atccgcgcgt
     7561 tcagaagcgc ctgggagaaa tgctgaacga ccaacaacat cgggagctga cggggttaat
     7621 gcaggagtgg ctgacaatag tggacggaaa agcctggtca tttaaacaaa gggagttcat
     7681 atgctgaagt tgtctgaaga gcagtgtgtt ggtatggaca aaagggaaga tgatgatttt
     7741 gtttgcgaca ccgcgctaag actgaagaag aaatttccgg gaattactga atcagatgac
     7801 acgttccatg ttcgcctgag aaaagcgctg gattatgcca atacccttcc gcttaaagag
     7861 aaaaacgtga agcgggactt cttgtttctg gaggccttct ggcctggttt ttatctgaaa
     7921 cccgaagtgg ataaatggtt gagaacgccg aatgcttatt caactgacca gcgggttgaa
     7981 gattataagc atgtgatgat aaacagagaa aggagggagc tctgatggtt atggtcctga
     8041 caggggctgg tatggggagc gcagctaccg gaggtgttgg tatggctggt gcctcaaccg
     8101 gaacagcagt atttcccgga gcaggaagcc gcgatggaga ctgggaagaa gataatatat
     8161 cttatcctgg ctcaggcagt ggttcgcgtc agggtaacgg agggttttat tatcagggga
     8221 aacagtatga tccaattctg aatgagccga tggttgtcag cgcagagacg catggtgatt
     8281 tctgggaaga tgatatggat cattcagaaa cagtaaacta tgaaggtatt gatagcaaag
     8341 ccgaagtaca ggcgaagacg gatgagaaaa cgtgtgagaa ctgcccaccg gaagggaagg
     8401 taatgccgat gataaggcgt tgctcgcgct ggagtatggt aacaatcagc taccagacaa
     8461 gaatatgtgg cacgttttat aatccggaga cgcaaca
//
LOCUS       DAZGBC010000185         8445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGBC010000185 DAZGBC010000000
VERSION     DAZGBC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.186"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..980)
                     /locus_tag="ACHK1J_004321"
     CDS             complement(<1..980)
                     /locus_tag="ACHK1J_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7913511.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIAS"
     gene            complement(1007..1627)
                     /locus_tag="ACHK1J_004322"
     CDS             complement(1007..1627)
                     /locus_tag="ACHK1J_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7913512.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(1894..2559)
                     /locus_tag="ACHK1J_004323"
     CDS             complement(1894..2559)
                     /locus_tag="ACHK1J_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO7913513.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEDIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(2700..3341)
                     /locus_tag="ACHK1J_004324"
     CDS             complement(2700..3341)
                     /locus_tag="ACHK1J_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO7913514.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            4044..4916
                     /gene="repA"
                     /locus_tag="ACHK1J_004325"
     CDS             4044..4916
                     /gene="repA"
                     /locus_tag="ACHK1J_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7913515.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     SSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            5821..6105
                     /locus_tag="ACHK1J_004326"
     CDS             5821..6105
                     /locus_tag="ACHK1J_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO7913516.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            6105..6380
                     /locus_tag="ACHK1J_004327"
     CDS             6105..6380
                     /locus_tag="ACHK1J_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7913517.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            6486..6689
                     /locus_tag="ACHK1J_004328"
                     /pseudo
     CDS             6486..6689
                     /locus_tag="ACHK1J_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6958..7224)
                     /locus_tag="ACHK1J_004329"
     CDS             complement(6958..7224)
                     /locus_tag="ACHK1J_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913518.1"
                     /translation="MITGNGINTVTVNGKVKHITELDYITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTRRLLLTRNISGSVMKK"
     gene            complement(7224..7700)
                     /locus_tag="ACHK1J_004330"
     CDS             complement(7224..7700)
                     /locus_tag="ACHK1J_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913519.1"
                     /translation="MCDSEAEKILFILNKHERAYRCQYPVYIFLAIVLWILPILLIIY
                     LHFCIGIGFCIAWTAYYFLSLFEYFVKYFFPLDREDLLNLMDLAKDSPDAKQELLERL
                     LSGKRMTGRDEMDIKRILKTKEALDNIGNFIDKQQLTNNDTPKKSTVPNEDKKGIR"
     gene            complement(7756..8358)
                     /locus_tag="ACHK1J_004331"
     CDS             complement(7756..8358)
                     /locus_tag="ACHK1J_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7913520.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
BASE COUNT         2139 a         2068 c         2140 g         2098 t
ORIGIN      
        1 tggctggcga tttcggttgc ctgctgttgc tcaatttccc gtatggccac ttcccgttcg
       61 gcttcgacct ggcgggtacg cactatctgc tgtcgttcga tttcggcttc ctctatctta
      121 cgttcagcca gaattctcgc ggattctgct tcacgacgtc gttcagcttc aatagcagca
      181 atactggcac tctgctctgc cgtacgggtt tttacccgtt gttgttgctc cagagtcatg
      241 aaggcttcct gctgttcaat ctccagacgt cgggagagtg catcccggtt tttttccctg
      301 atggccactt caacatcctg ctcaacttcg ttacgttcgc ggcgacgacg ttctgtttcc
      361 tgggtcagaa gggtcaggcc ttcagcatca aaggcattgt caggattaaa atgttcccgt
      421 gcagtctggt tgaaacttgt cagcgacaca ctttcaagct ccagaccgtt tttggataag
      481 tcttccgcaa cggtgttctg cacaccctga acaaagttct cgcgggcatc ctgtaaatcc
      541 tgcatgctca ttctggccgc cgtggcccgc agggcatcaa caaatttatc ctcgaccaga
      601 ctacgcagat cttcaggtgt gagcgtgcgt tgtcccagcg tctgggctgc agtggatatt
      661 ccttcggctg atggttttac ccgaagaaaa aaggcaacgg caacatcaac acgcatgcgg
      721 tcgcgggtaa tgagactttc agccgctgca cgactgactt ccagcttcag ggtgttcatg
      781 ttgacgggaa ttgtttcgtg aaaaatgggc ataacaattg caccgccact cattaccact
      841 ttttggcctc ccagacctgt gcggacaaag gcctgctctg ccgatgcccg acggtacagg
      901 cgggcaaata tcagcccgat aacgaacagg gtgaggacca gcgcaataac ggtaaacaac
      961 catcccggaa gattaaacat agtgagaacc tctgtatatg tcgggattaa agaacatccg
     1021 gccagggatt cagttcgccc agccagcggg aaccggaaag acggcagaca ataatcactt
     1081 tgtctcctcg tctgaattct ttcccggcct ccggctccag caacaggtaa tgaacctgcc
     1141 caaagcggtc tgtgaagcga ccttcacagg gggaacccgc cactgcgtga taaccggtga
     1201 tggtggccat acacccgatg aatgcctcct cggtgagcgc cgttgtttca tcacggggta
     1261 accagggggc aatcagtctg ccggtgcggt gtacgagagg acatgtcagc agcaggcaaa
     1321 gtggtgcaag gagaatgttc ggggcaggag cctgcagcag atgaataata agaccctgca
     1381 ggagaatgcc ggatatacca aaaaaacctg ccagcataca gaggacgatc agcaggggga
     1441 tccgtccgat gttcagccag tccagcgcct ctcctgtcag cccctccggt aaatcaggtg
     1501 tatccatagc tgaagagagg gagtgtccgc agagcagggc gataagttca gcgataccag
     1561 taagcagaac aaaagtcagg gctgccagat agggtgcgtt atacgcggta agtaacgtca
     1621 gtgtcatttt tcctcctgtg gcaggtcagt caaacaacat tatggcgtga agcttccgct
     1681 tctttaattg tcagaagaag catatcgtca gtcatcagaa gacaagggga tgtctgcagg
     1741 aggggatgat aacaacagaa gcctcatgcc ggaatgcgct ttatgacaac cggagaggac
     1801 ggataataaa aaatgggaca tccacatcag caaaccgcga tttgcgaaaa aaagtcagcg
     1861 gagagtggct gccgttgtgg ctgtcccggt atttcagtgt aacaggtgat tgctgtgatc
     1921 tatgccccat tcggctttcg gtaaacctga atgccccaca tgaagaatgc gtgacagcat
     1981 gtttctgccg ctgccgttca ggctgcattc gcgtccgtgc gcgagtataa aaccttcatc
     2041 ccggaatata tcttccactt ctgaagattc gagggatgcc ggatgaatta atgaaaatgc
     2101 caccggtctg agcattctgc ccgtggtgga aaaggtgtct ctggtactgt tcagctgcag
     2161 gatatgacgt ttacctgttt gcgcacggga aagtgtctca ctcagcagct ggtcgttcca
     2221 tgcgcacgtg ccaatctgcc atcccagact tctgcacgcg gaaagagccg agcggaagcg
     2281 gtacaggtcg tataatgctg cgttattgcc actttccagc agtgatttca gggtgtttgc
     2341 taccggtatg tctgaattgg tggccagcag ttttcgcagg gtagtgaact cttcagccat
     2401 tcctctgaac tcgcgcatct gacgggcagt gtcaagccag ttcagcaggt aacgccggcg
     2461 acgccgttcg ttgtttatgt tgtccggggc ggtggagagt gcttcgagca tattctccag
     2521 taacagaatg acagggtttt cctgggatag aatggtcata aaggcgtcct gaaaaaatgc
     2581 ccgacagccg ctctccgtac accctcaggt gtcaccggtg ccgggcattg tggaaacaga
     2641 agggaaaaaa atcccggcca taaaggccgg gaaaagtcgt caaattaaga cagcaatttt
     2701 catgtcaacg ctccgtcatc agataccagg tgcaggagca gggcgatcct cgactccggc
     2761 agaggctgtg catcaagccg gaggaggtac tggtaacgcg cttttgtcag ccagagggtg
     2821 gaggcagcat ccagctcctc cctggctgcc ggattgagcg caggatccgg gtacactttc
     2881 atcaaagact gaacgatatc ctccctgacg gttttgattt caccggccat tttcacgaag
     2941 tccagaaagg cactgtgacg gcgaaggtga gtgaagctgt ggcgttcggg gtctgctttc
     3001 aggaaaaagt aaccgggaaa cacggcgtgt atatgtcgac ggtaaccaca gactttatcc
     3061 gtgcggcgga cctttttcag tatcaggggt gtccagggaa ggacgtgttg ttcggaaagc
     3121 caggaaaaga gatgttcgcg gtttttgccg gcagggatgt actgagccag ataccagttg
     3181 cgttgatgta attcactgat attcaaaatc cggaacgctc cgcgaaaatc gatccccgca
     3241 gaggggcttc gatgttgttg tgaacggcga atacataaag catgtgtcgg aaacagcggc
     3301 tgaccctcgt tcagcccggc ctgcagttct gacaggcaca tgatttacgc actcttgtaa
     3361 gccttgtgga cggtttcgag gcgggcgaaa accgacaact cctgcaaaca ggattaatgg
     3421 ctggcaaata tattacataa ggatgtatct gtggcaagag tgaagataag cagttaaata
     3481 gatcgttata tttgataaag cagcatatta tgctgatttg tgtgcggtgt ctggaggatt
     3541 agatcgttta aaaattttag gcgatctctt ctactaaaaa tgatgaaaaa cacagcataa
     3601 aagatcgttt gtggaattgt ctattttttt gacatagttc aaaaaaacga cgttttttag
     3661 gtggttaatg agaggcctgg taaatcagag aaatcgtgtc ggcttttcaa agcggtggaa
     3721 aaggggtata ttgcggatcg ttattcagtg gcttttggga tcctcgcggt ccggaaagcc
     3781 agaaaacggc agaatgcgcc ataaggcatt caggatgtat ggcagaaacg acggcagttt
     3841 gccggggccg gaaggctgaa aaaagtttca gaaggccata aaggaaaacc cccataatct
     3901 tcctgtcgaa cttggcggaa cgcacagaag attatggggg cctcacagaa tacgggataa
     3961 gtatatatga aaccgtgtca gagattcaac ccttcgcagt gtattaacgc acggcattct
     4021 gtttccgcca taagcgacag cttgtggcgg ccctgaacca gacctcattt actgaaagca
     4081 cataccgtca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaagat gccggaacgc
     4141 tgaagttctg cgaaaaactg atggaaaagg cggtgggctt cacctcccgt tttgattttg
     4201 ccgcgcatgt ggcgtatgcc cgttcgcgcg gcctgcgccg gcgtatgccg ccggtgctgc
     4261 gccgccgggc gattgatgcg ctgctgcagg ggctgtgttt ccactatgat ccgctggcca
     4321 accgcgtcca gtgctccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     4381 aggccgggaa actctccatc acccgtgcca cccgtgccct gaagttcctg tctgagctgg
     4441 ggctgataac ctaccagacg gaatacgacc cgacaattgg ctgtaacatc ccgaccgaca
     4501 tcacgttcac gccggcactg ttttcctcac tcgatatatc agaagaggcg gtggcttccg
     4561 cacgccgcag ccgcgtggaa tgggaaaaca agctgcgcaa aaagcagggg ctggatgcgc
     4621 tgggtatgga tgaactgata gcgagagcct ggcgttttgt gcgcgagcgt ttccgtagtt
     4681 atcaggcaga gcttaagtcc cacggcatga aacgtgcccg tgcgcgccgc gatgcaggca
     4741 ggacccgtca ggacattgtc accctggtga aacggcagct gacacgtgaa atcgcggaag
     4801 ggcgtttccg cggaagcctg gaggccgtga agcgcgaaat cgaccgtcgc gtgaaggaac
     4861 gcatgataat gtcgcggaac aacaactaca cacggctggc cactgcctca ccctgagttt
     4921 gacctcatct gaataatccg gcctgcgccg gaggcttccg cacgtctgaa gcccggcaac
     4981 gcacaaaaaa gcagcacagc acacaaaaaa cagcctcatc atctgccttc cggctcgcct
     5041 tgctccccct gtttttcacg caaaacacgt ctcacagacg gggaattctg tttatccaca
     5101 tttaactgca agggacttct ccgtaaggtt acaaccgtta atgctataaa gcgccagccg
     5161 caggcgttac agggtgcaat gtttctttta aaccccagta ctgtatttcc tttaaactac
     5221 ttaattactt ccatttgaaa agaaaaccta ttcagtgcct gtcctgtgga caagcaaata
     5281 tgcacctccc acgctaacgc gcgggccccg aacctcaccg ttctgaaacc acagcaaaaa
     5341 gacatcagga ataaaaacat cacacaaacg cagcaccgtg ctcacctctc ataactgaaa
     5401 agcgaggccg cccccgcccg aagggcggga acaacatcgc ttttaattat gaatgttgta
     5461 actacacctc atcatggctg tcagtgttct ggctggaagt atcgagtaca cgctcgtaag
     5521 cggccctgac ggcccgctaa cgcggagata cgccccgact tcgggtaaac cctcgtcggg
     5581 accactccga ccgcgcacag aagctctctc atggctgaaa gcgggtatgg tctggcaggg
     5641 ctggggatgg gtaaggtgaa atctatcaat ccgtaccggc ttacgccggg cttcggcggt
     5701 ttcacacctg tgctacatgt aaccaccgta tgccgcctta catgcgctga cgcgcattat
     5761 ttgccttgtt ccgtctgaaa gtaatcacta tgattaaata tgattaacaa ctaatcggat
     5821 atgcaaatga aaaacaatac cgcacaggca acaaaagtca ttaccgcgca tgtgccatta
     5881 cctatggctg ataaagtcga ccagatggcc gccaggctgg aacgctcccg gggctgggtt
     5941 atcaaacagg cgctttctgc atggcttgcc caggaggagg agcgtaatcg cctgacgctg
     6001 gaagccctgg acgatgtgac atccggacag gttatcgacc atcaggctgt acaggcctgg
     6061 gcggacagcc tcagtactga caatccgtta ccggtgccac gctgatggaa ctgaagtgga
     6121 ccagtaaggc gctttctgat ttggcgcggt tatatgattt tctggtgctg gccagtaaac
     6181 ctgcggccgc cagaacggtg caatccctga cacaggcacc ggtcattctg ttaactcatc
     6241 cacgtatggg agaacagttg tttcagtttg aacccaggga agtcagacgg atttttgctg
     6301 gcgagtacga aatccgttac gaacttaatg gccagactat ttatgtattg cgcctgtggc
     6361 acacacgaga aaacaggtag attgtgtgtg gggacaactt aacatagcct gcagatatta
     6421 attctggaac attattatca ggccagatat ttttcattcc attaaataat cttgtaggag
     6481 ataaaatgaa ttacgatgaa ataacgaaaa ttaccgctga acgtattagc gactacatga
     6541 ccgaagcagt taatacagac tctatagccg ttgcggaaat gtttcataac gctgcctggg
     6601 gagtgcgaac attgtggttt gaactcgtca caaaaataga tatagatata cataaaaaga
     6661 acaggtatgc gagttatgat cttagaagga aaatcgaaat gcagcatgaa gagtttcaga
     6721 aaatgacaga gcgcgagcaa gtgcctttat tgaaatcgcc agaataatag aaacagtaca
     6781 agtacattgc tgtattgtgt tttccttgaa tattgaatcg cggtgcccat gtactacgat
     6841 tcctttcgtg ttgtttatca ttgcacactt tccctgactg agcgtaaaca ctgactactt
     6901 tcccctcaaa ctcctggacg gcgaatgcaa aattggtctg ttccagcata gaatatgtta
     6961 ctttttcatg accgaacccg aaatattgcg cgtcaataag agacgtcgtg taaactcagt
     7021 ccttttatct ggcaggttaa cccctatcaa tcttaggatg aaaccacgcc atcctctgtt
     7081 tgcctggtta ttaatttcat aaagacgatt gttttcctct cgtagttttg tccactctaa
     7141 acaaagtgtt atataatcca gttctgtgat gtgtttcact tttccattga cagttacagt
     7201 gtttattcca tttcctgtga tcatcatctt atgccttttt tatcttcatt tgggaccgtg
     7261 cttttcttcg gagtatcatt attcgttaac tgctgtttat caataaaatt cccgatgtta
     7321 tcaagtgctt cctttgtttt tagaatgcgc ttgatatcca tttcatcccg cccggtcatt
     7381 ctttttccgg acaaaagacg ctcaagaagc tcctgtttcg catcaggaga atctttggcg
     7441 agatccatca ggttaagcaa atcttctcga tcgagaggaa agaagtattt tacaaaatac
     7501 tcgaaaagcg aaagaaagta gtatgctgtc catgcaatac agaaaccgat tcctatacag
     7561 aaatgcaggt atataatcag gagaataggt aaaatccata atactatagc taaaaaaata
     7621 tatacggggt actggcatct gtatgcccgt tcatgtttat tcaggatgaa taaaatcttc
     7681 tccgcttctg aatcacacat aacattctct tcccattttt tctgatacgt aatgtccacc
     7741 tgttatcagg agtgtttatt gcccagaaat attctcctgc tgtgcctgca tcctgagcca
     7801 cttttgttct aatgtcgcca gtctgcgacc ggcactctgt gcttcagagt ctgagacctg
     7861 ccctttgggg tgaccatcca ggccataacg tgggacgccg gcgacagctg tccgctgcag
     7921 atatacgcgg cgggatgtga tgcaggacac tgcacagagc cattcgtcca tggtcagttc
     7981 tgtctccggg tgttctgcca gaaatgccga aatatcattg tttatgccaa ttttcagggg
     8041 aataaatcca ccaccttcag cacgaagatg aggaaacagg gcctttattt tttccacgca
     8101 ctcgtgagta tactcacggg tccagtggga gggacgtgga aaacggtgtc ttttcttttt
     8161 cttcttcctg gctggcgctt cggggatatg tgtttcgacg gcagatgaat ttattccggc
     8221 gggggctgat tctgccgtct gttcagtaaa tacaggtgcg ataatatctg ttttttcgga
     8281 taaggacggt agcgaaaaag tacgacgttt tttcaccaca atgacgggag ttttcctttc
     8341 ttcctgattt tttcccataa atacctcggc tggctgattt tactctttaa taatatcaca
     8401 attcataaat tagtaaattt tccatcaggt gatttttcat ggcta
//
LOCUS       DAZGBC010000186         8331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGBC010000186 DAZGBC010000000
VERSION     DAZGBC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.187"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(279..929)
                     /gene="zinT"
                     /locus_tag="ACHK1J_004332"
     CDS             complement(279..929)
                     /gene="zinT"
                     /locus_tag="ACHK1J_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO7913521.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1186..1821)
                     /gene="msrQ"
                     /locus_tag="ACHK1J_004333"
     CDS             complement(1186..1821)
                     /gene="msrQ"
                     /locus_tag="ACHK1J_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO7913522.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALIGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1822..2826)
                     /gene="msrP"
                     /locus_tag="ACHK1J_004334"
     CDS             complement(1822..2826)
                     /gene="msrP"
                     /locus_tag="ACHK1J_004334"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO7913523.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLHSWFKGNDRPPAPAGKPLDFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2934..3347)
                     /gene="hiuH"
                     /locus_tag="ACHK1J_004335"
     CDS             complement(2934..3347)
                     /gene="hiuH"
                     /locus_tag="ACHK1J_004335"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO7913524.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3480..4151
                     /gene="hprR"
                     /locus_tag="ACHK1J_004336"
     CDS             3480..4151
                     /gene="hprR"
                     /locus_tag="ACHK1J_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO7913525.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4151..5509
                     /gene="hprS"
                     /locus_tag="ACHK1J_004337"
     CDS             4151..5509
                     /gene="hprS"
                     /locus_tag="ACHK1J_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO7913526.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEVCF
                     NVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(5617..6468)
                     /gene="hchA"
                     /locus_tag="ACHK1J_004338"
     CDS             complement(5617..6468)
                     /gene="hchA"
                     /locus_tag="ACHK1J_004338"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO7913527.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(7061..8248)
                     /gene="ompC"
                     /locus_tag="ACHK1J_004339"
     CDS             complement(7061..8248)
                     /gene="ompC"
                     /locus_tag="ACHK1J_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7913528.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDMSYARIGFKGETQIADQFTGYGQWEFNIGANGPESDKGNTATRLAFAGF
                     GFGQNGTFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANSVATYRNNGFF
                     GQVDGLNFALQYQGNNEKSGLFDQEGSGNGNGRKLAKENGDGYGMSTSYDFDFGLSLG
                     AAYSNSDRTDNQVHKGTHNTRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAET
                     RNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQGN
                     HHYTNKDLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLV
                     YQF"
BASE COUNT         2286 a         1739 c         1944 g         2362 t
ORIGIN      
        1 atgtggtttt aagaatttgt tatttaaaaa attgatagtg cacattgtaa ccagccaata
       61 taagcaatgg cgtgagcaca ggtatctttc tctgttggcc gtattgtgta tggaatccgt
      121 tattggttaa ctcaatgcgg gttagcttat taatatacag acacgtcata gtgatgagcg
      181 tgtctgtagc tagcttgcgt tcagtggcgt ttgtggatgg accagtggca gttatgatga
      241 cttactaaag cggcatcgag gcgttatcat gagaatactc aatgagacat catttcctcg
      301 accacttctt cgctactcaa ctgatatgga taatacgttg gccagttttc catttcattt
      361 aataaagatt gctgtgaatc attacccata aaaatgtgaa aatgagatga ttttcgtggt
      421 gcaattatat gatcgctaaa ttgtatatat ttaggggctt tgctttcagg atctttacat
      481 tcgaataagt aacgaacgcc tttcttgcct gatttatagg tgagtatttt gtatccatcg
      541 taatcatatt tacaggatgt tgtttcatta tttctatgga attcaacaat gccgtcctca
      601 atgccaatca tctcgatatc tgttgcataa cctttgtgat aataatcttt aatttcagca
      661 aatgttttag ttttatctgc atccgctttc ttctgaaaga cggggtcaag tttgccactt
      721 tgcagtaaag gataaacgga ttgccagact ccatcccagt cactgagcgt tcggttttgt
      781 acattggcat catcaaaaac accattagca gctttttgtt cgacctctgt taagggtttg
      841 ccgtgtgagt gatgaccatg cgaaaaggca ggagcgctaa caataaagac acctaaagca
      901 acagccagtt tgtgaagacg aatcgccaaa acagttttct ccagagtcat tgtatatgat
      961 gtgtaatgtt atattgtaac atatgacaaa atgcaacaac tcttaggaga tgagagcata
     1021 gttttctcag gtgtacttat ctatcttatt gaaataaaat gaattgtgtt gataatttga
     1081 ggttgatgac aaacgaaaca taacctgatg cgatctgcgt aggccggata gggcgctcgc
     1141 gccacatccg gcagggataa cgcgtacttt gtcggcaata tgaagttata cagataattt
     1201 gttgtgaacc tgcttgcgta accggttaaa aagggaacgc aacttcttat accgtaaggc
     1261 taaaagcagt acagccagcc cagcgtagat gagcggctgc ggtgaaataa tcttcaccga
     1321 ccacagataa tgtattgggg ccaggatcgc gacaagatag acgaaattat gcaactgttg
     1381 ccagtgcttg ccgagttttc gctgcatcgc ctgggttgaa gtaaacgcta aagcaagcaa
     1441 aataacccag ctgataattc ctaacgttaa ataaggccgg gtaattaact ccttgcctat
     1501 taacgccaga ttattcacgc ccagctccag caatgcgtaa ctggttaaat gcagtgtcgc
     1561 ccaggcaaag caccataagc ctaacaggcg gcgagtgcgt atcaataacg gctgttttgc
     1621 gtagcgtgcc agaggtgtga ttaacaaggt cgccagcaaa aatttcagtg cagtgcgacc
     1681 ggtaaaatgc tgaatatctt tcactggatc ggcacccagt ccaccgtgat tgatcgccca
     1741 gaccagccag agaaacggca acaatccggc aagatgcagg caaactttca gccatgtcac
     1801 ctgttttgct gtcagacgca tttagaagtt ctcccgcaga tccaggccac gatacagcga
     1861 tgccacctgg tcggcgtaac cattaaacag tagcgttggc tggcgctgta catcgaggat
     1921 gccgcctgaa ccaataaatc gttcggtagc ctgtgaccag cgtgggtgat caacatgcgg
     1981 attaacgttg gcgtaaaaac cgtattcgtc aggcgctgcc agattccagg tggttggcgg
     2041 acgctcgcgg gtcagcttaa tactgacgat cgatttaatc cctttaaagc catatttcca
     2101 cggcacaatc agtcgcaccg gcgcgccatt ttgtggcggt aacgccttgc cataaacacc
     2161 tacggtcatc agtgtgagcg gatgcattgc ttcgtcgaga cgcaatcctt cgacataagg
     2221 atatttcagc ccgccgccga taaagcggtc ctgctggcct ggcatctgtt ccggtgcata
     2281 aattgtttcg aaagcgacat acttcgcatt gctggtgggt tcggcaagcg ccagcaattt
     2341 gtgcagtgga aaaccaatcc acggcaccac catcgaccat gcttctacgc agcgcatacg
     2401 ataaatacgc tcttccagcg ggaagcgacg ggttaagtca tcatgatcga gggtcaatgg
     2461 ttttgccact tcgccgctga ttttcagtgt ccatggatcg gttttcaggc taccagcatt
     2521 agcggcggga tcggctttat ccagcccgaa ttcatagaag ttgttataac cggagacttt
     2581 atctgctggc gtcagtggca ggttattttg ccaggcggca ggcttgctga agtccagcgg
     2641 ttttccggcg ggggcgggcg gacgatcgtt ccctttaaac cagctatgca gatcggcatg
     2701 tgcagcgtgg ggcaaagaaa gtgcagctgc gctgatgccc agtgctttta acacctgccg
     2761 acgcttcata aagaataccg actcggccgt aacatctgat tcttttaaaa attgattctt
     2821 tttcataagc catcacactt tttgtcgtta gccagcaaga gccgtttact gttaatctgt
     2881 cagggagtgt cctccctaac agtagcagag atctgctttt acatatttat gcattaactg
     2941 ccacgatagg ttgaataccc atattggcta agtaataaag gcacatgata atgctcgttc
     3001 actttattaa tatgaaattc aacggggatc tcagggaaga aactttcaag attttgtttc
     3061 ttgaaatagt ccccggtttt aaatacgaca cggtaatcgc ccgtagttgc agtttgctcg
     3121 ggccacagtg ccttaattcg tccatcctta tctgttttgg cagtattaag ttgtaaccag
     3181 ccgttgtccg ctttcttttc aagagtgact gtcacgtcgg cagcgggttt tcctgtttgc
     3241 tggttcaaaa tgtgcacgct aagaatgttt tgttgtgcag catagaccaa agaaggtaat
     3301 gaaaatgcag ccgttactac ggagagtact aaataacgct ttaacatgtt tatatccttg
     3361 tcatgtgaat gagttttgag gatagcgaaa aagcgtccag ggtaacctta cagcagcatt
     3421 acaattttgt aatgaaccag gctgtttcta taacatatga tttatggcat attattttca
     3481 tgaagatttt acttattgaa gataatcaaa ggacccaaga gtgggtaacg caggggcttt
     3541 ccgaagcggg ttatgtcatt gatgccgttt ctgatggcag agatgggctt tatcttgcgc
     3601 tgaaggatga ttatgcattg atcattctgg atattatgct tccgggtatg gatggctggc
     3661 agatcttaca aacgctaaga acagcaaagc aaacccctgt tatttgcctt actgcaaggg
     3721 attctgtcga tgacagagtc agagggctgg acagtggggc aaatgattat ctggtaaaac
     3781 ctttttcatt ttctgagttg ctggcaaggg ttcgggcaca attaagacaa catcacgctt
     3841 tgaattcaac attagaaatc agcggcttaa gaatggactc tgttagtcaa agtgtgagca
     3901 gggacaatat cagtattaca ctgacgcgca aggagtttca gttactttgg ctgctggcct
     3961 ccagagctgg cgagattatc cccagaacgg ttattgcgag tgaaatttgg ggaatcaact
     4021 ttgatagtga taccaatacg gtggatgtcg ccattcgcag gctccgcgca aaagttgatg
     4081 atccttttcc tgaaaagcta attgccacaa tccgtgggat gggatattcg ttcgtagcgg
     4141 taaaaaaata atgaaaagat tatctataac cgtccgttta accttgcttt ttatattgct
     4201 gctgtctgtt gctggcgccg gaattgtctg gactctctat aatggcctgg caagtgagtt
     4261 gaaatggcgc gatgatacaa cactcattaa ccggacagca cagatcaagc agttgttaat
     4321 tgatggggta aatccagata cgttacctgt atactttaac cggatgatgg atgttagtca
     4381 ggatatcttg atcattcatg gtgatggcat caataaaatt gttaaccgga caaatgtcag
     4441 tgatgacatg ttaaataaca tacctgctag tgagacaatc agcgcagctg gcatttacag
     4501 aagcattatt aatgacactg aaattgatgc tttacgaatt aatattgatg aagtttcgcc
     4561 atcattaacg gttactgtgg ctaaattggc ttcagccaga cataacatgc ttgaacagta
     4621 taaaattaat agcattataa tttgcattgt cgccattgta ctttgctcag tattaagtcc
     4681 gctgttaatc agaacgggat tacgagagat caaaaagttg agtggtgtaa cggaagcgct
     4741 gaattataac gatagccggg agcctgttga ggttagcgca ttaccgagag aactaaaacc
     4801 tcttgggcag gcgttgaata aaatgcatca tgctttagtc aaagattttg agcgtctaag
     4861 tcagtttgct gacgatctcg ctcatgaact tagaacgcca attaatgcat tactgggtca
     4921 gaatcaggtt acgctaagtc aaaccagaag tatcgctgaa tatcaaaaaa caattgccgg
     4981 aaacattgaa gagctggaaa atatttcgcg gttaacagag aacatactgt ttcttgccag
     5041 ggcagataaa aacaatgttt tggtgaaact ggactcgctt tctctcaata aggaagtcga
     5101 aaatttgttg gactatcttg aatatctttc agacgagaaa gaggtttgct ttaatgtcga
     5161 gtgcaatcag caaatctttg cggataaaat tttactgcaa cgaatgttat cgaatcttat
     5221 tgttaacgct attcgatatt cgccagaaaa atcgcgtatt catataacca gttttcttga
     5281 taccaacagc tatcttaata ttgatatcgc cagccctgga acgaaaatta atgagcctga
     5341 aaaactcttc cgtagatttt ggcggggaga taattcgcgt cattccgtag gtcagggact
     5401 tggtctttct ttagtcaaag cgattgccga attgcatggg ggaagtgcta cgtatcacta
     5461 tctcaataag cataatgtgt tccggattac gttaccgcaa agaaattaac atagggcttc
     5521 agtacgccat aaattaaccg tgaaaaaacg cagccacgtc gtgagtacta acgcggctgc
     5581 gattgattat gcgcttacat tcaaacgtaa cagggattaa cccgcgtaag ctgccagcat
     5641 ttcctgcgcc gccagtttac ccaacgcatt cgctgcaaaa ggactgtcgc cggtgagaag
     5701 tttacggtcc ttatgtactc gcccggtgat gtcgtcatta atgatattca tgcccatttt
     5761 cttcagttct tcgccgaagt accaggtgag atgacccggc atatagccaa tctctggcgt
     5821 ttgtttgtct gcggcgtctg ggaatgcgca aatggaataa ccattcagtg ggttatcgcc
     5881 gtggcgaagc gccagaaaag ccgccgggcc gtggcaaagg gagataacaa aacggtcatt
     5941 tttgattgcc cactgtaaag cggcagccac gtcctggctt tcaggtaaac caataagtgc
     6001 gccatgacca ccaggaacaa agattgctgc atattcgcta tcagcgttga ggctggcgac
     6061 gacatccgcg agtttcttcg gattgcggaa caacgattta tgctgctcaa agaatggcat
     6121 tactttttca tctttgtgcg gcatagccca gtattcaaac ttggtcatca gaccggaaat
     6181 ggtcgccact tcgaattcga aacctgcagc atggagatga tacaacggca gcaacgtttc
     6241 aatcggatgg ttaccggtcg agaacagttt tccgttatcg gtcggcaaat aacgttcgtc
     6301 cgcggcgatc accagaattt tatgtttacc gcgatacggt tttggatagt ccacgccatc
     6361 aagatcagag acaggactgg tatattggct aagcgaatat tctgaaggga agaatgcatt
     6421 atcttcagca atatcgacct gcggattttt acttgtttga acagtcatag tgtattcctt
     6481 attgttgctt agttagggta gtcactatat ttgcgactga gctggtcgct atttttaacc
     6541 cggctagaaa attgagtcaa tatatcgaaa gcggtagtcg gcgggtgtaa gtatgtaatg
     6601 atgatgctgc actggtgttg aatattattc acgccaaatt ttccttattg agtaacgtcg
     6661 tcgcaagtgg cctcaatgat tttagaccat tatcagaggt acggggtggc ggggagagat
     6721 ttagtgctgt ggaatattgt ggtgctgatg aatatttata tccaggattg gcagagaaaa
     6781 agcttttttc atgcactatt tttgcaaata tttctatatg ggaagtgtgt ttgttgcgga
     6841 ttaattcata aagaaatgta tttggggtgt tggctggaag agtagctggt agaagatatt
     6901 gtgccaggag gggcagggta aggtggtgta tcgatatgct ttggcaattg cgtgtgccgc
     6961 agagggagat gaaatgtcaa taaaaaagcc tgagttcgat gagggacgaa ctcaggctag
     7021 tgttcagttt tacagtaaca cgccgcagcg agttggctga ttagaactgg tagactaagc
     7081 ccacagcaac gatatcatcg gtagcgatgc cgttttcttt gtagaagtcg tcgtcgttgt
     7141 ccagcaggtt gattttgtaa tcaacatagg tggacatgtt tttgttgaag tagtaggtca
     7201 taccaatatc aacatattta accagatctt tgttggtgta gtgatgatta ccctggctgt
     7261 cgaagttatc actatttaca ccgcctaaat ccttaccttt agattgcagg taagcgatgg
     7321 acgggcgcag accaaagtcg aactgatact gagcaacaac ttcaaagttt tgggttttgt
     7381 tagcgatggc atcagcatca ccgtaaccag tcatgttacg ggtttcagca tacatagctg
     7441 ctaagtaaac gttattagcg tcgtatttag caccaacggt ccatgcttct gctgtttcac
     7501 cgccagcagt tgcatcgaag cgatctccat aacgagtgtt atgagtacct ttgtgaacct
     7561 gattgtcggt acggtcagag ttagagtaag cagcgcccag acttaaacca aagtcaaagt
     7621 cataggaagt ggacataccg taaccgtcgc cgttctcttt agcaagttta cgtccattac
     7681 cgttacctga accttcttga tcaaataatc cgcttttctc gttgttaccc tgatactgga
     7741 gtgcaaagtt cagaccatca acttgaccaa agaaaccatt gttacgatag gttgctacgc
     7801 tgttagcacg accgttcatg aagttgtcag cgccagcata ggtatctcca ccaaattctg
     7861 gcagcatatc ggtccaagct tctacgtcat atacgacacc gtagttacga ccatagtcga
     7921 aagtaccatt ctgaccaaag ccgaaacctg caaatgccag acgcgttgcg gtattacctt
     7981 tgtcgctttc aggaccgttt gcgccaatgt taaattccca ctgaccataa ccagtgaatt
     8041 ggtcagcgat ctgagtttca cctttgaaac cgatacgggc atatgacatg tcgccatcgc
     8101 tgccagcatc atcagagaag tagtgcaggc ccgctacttt tccgtacaaa tccagtttat
     8161 taccatcttt attatagact tcagccgcat ttgctgcgcc agcaactaat aacgccggga
     8221 ccagcattgc cagaactttt cttttcatta tgtattccct tgtgattata atcttcatga
     8281 atatatcaat aagtgccgtt atccaaaaaa aagcacattt ggatactatt c
//
LOCUS       DAZGBC010000187         8312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGBC010000187 DAZGBC010000000
VERSION     DAZGBC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.188"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..1378
                     /gene="adeQ"
                     /locus_tag="ACHK1J_004340"
                     /gene_synonym="yicO"
     CDS             44..1378
                     /gene="adeQ"
                     /locus_tag="ACHK1J_004340"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO7913529.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1431..1883
                     /gene="yicN"
                     /locus_tag="ACHK1J_004341"
     CDS             1431..1883
                     /gene="yicN"
                     /locus_tag="ACHK1J_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO7913530.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(1999..2322)
                     /locus_tag="ACHK1J_004342"
     CDS             complement(1999..2322)
                     /locus_tag="ACHK1J_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7913531.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LDVLL"
     gene            complement(2306..2659)
                     /locus_tag="ACHK1J_004343"
     CDS             complement(2306..2659)
                     /locus_tag="ACHK1J_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7913532.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2893..4077
                     /gene="nepI"
                     /locus_tag="ACHK1J_004344"
     CDS             2893..4077
                     /gene="nepI"
                     /locus_tag="ACHK1J_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO7913533.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLCGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            4279..>8312
                     /gene="air"
                     /locus_tag="ACHK1J_004345"
     CDS             4279..>8312
                     /gene="air"
                     /locus_tag="ACHK1J_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HFO7913534.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINHAHSDTTTSLILPNVKTIPYTLGALESPSTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTPSWMSGVNMFIDYDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDHDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEEPTLSLADSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQAKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNDNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGTAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGVIKVTVTL
                     KDSYENLVGGQRDAINQAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SDWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVRTAQIADLVVIKDGSVADGSTANTLRVRVSDAFGNALDGQTVSVLADNGAT
                     VAPTVITEPDGTVEISVTSQTAGVSAVTASINNSSLSRNVTFVADVRTAKIADLVVMQ
                     DGSVADGTTANTLRARVTDAFGNALAGQTVSVTAGNSATVASTVTTKPDGTVEISVTS
                     QTAGVSTVTASINSSSQSRDVTFVA"
BASE COUNT         1941 a         1970 c         2264 g         2137 t
ORIGIN      
        1 tttacaaaag caagggatta aatctcctcc aatggacaaa aaaatgaata atgacaatac
       61 cgattacgtg agtaatgaat cagggacgct ttcgcgatta tttaaactac ctcagcatgg
      121 gaccaccgtc cgcacagaat tgattgcggg gatgaccact tttttaacca tggtgtacat
      181 cgtttttgtg aacccgcaaa tcctcggcgc ggcacaaatg gacccgaaag tggtgtttgt
      241 taccacctgt ttgattgccg gtatcggcag tattgcgatg gggatatttg ctaacttacc
      301 cgtggcgctg gctccggcaa tggggctgaa cgccttcttt gccttcgtgg tcgtgggggc
      361 tatggggatc tcctggcaaa ccgggatggg cgcgatattc tggggtgcaa ttgggctatt
      421 tttgctcacg ctgtttcgta tccggtactg gatgatctcc aacattccat taagtttacg
      481 tattggtatc accagtggaa ttggattatt tatcgcctta atgggattaa aaaatactgg
      541 cgttattgtc gccaataaag acaccctggt gatgattggc gatttaagtt ctcacggtgt
      601 gttgttaggt attttagggt tttttattat aaccgtgttg tcatcacgtc attttcatgc
      661 cgcggtgctg gtttctattg tggtgacgtc ttgctgtgga ttatttttcg gtgatgttca
      721 ttttagcggc gtctattcca ttccgcctga tattagcggc gtcattggtg aagtagattt
      781 gagcggcgcg ttaacacttg aactcgccgg tatcattttc tcctttatgc tgatcaacct
      841 atttgattca tcaggaacat taattggtgt aactgataaa gctggcttaa tagatagtaa
      901 cggtaaattc cccaatatga ataaggcgct gtatgttgat agcgtcagtt cggtggcggg
      961 cgcgtttatc ggtacctcgt ctgttactgc ctatattgaa agtacttctg gtgtggcagt
     1021 cggtggtcgc acggggctaa cggctgtcgt ggtcggcgtt atgttcctgt tagttatgtt
     1081 cttctcaccg ctggtggcga tggttcctcc ttacgcaacc gccggagcgt taatctttgt
     1141 tggcgtgctg atgacttcga gcctggcgcg cgttaactgg gatgatttta ccgaatcagt
     1201 gcccgcattt attaccacgg tgatgatgcc ctttactttc tcgatcaccg aagggattgc
     1261 actcggcttt atgtcgtact gcatcatgaa agtatgcacc gggcgctggc gcgatctgaa
     1321 cctgtgtgtg gtggtggtcg cagctctgtt tgcactgaag attattctgg tggattagta
     1381 actggcgagg gtttccagat atcatgagtt ctgattacgc aggagaactc atgatctgga
     1441 taatgctcgc cacgctggcg gtagtgtttg tggttggttt tcgggtgctg acatccgggg
     1501 ccagaaaagc gattcgccgt ctcagcgatc ggctgaatat cgatgtcgta cccgtggagt
     1561 cgatggtcga tcaaatggga aagtcagctg gtgacgaatt tttacgttat ttgcatcgtc
     1621 cggatgagtc gcacctgcaa aacgccgcgc aggtgttgct catctggcaa attgtcattg
     1681 tcgatggtag cgaacagaac ctgctgcaat ggcatcggat tttacaaaaa gcccgccttg
     1741 ccgcgccgat taccgatgct caggtcaggc tggcgctggg ttttctgcgc gaaactgaac
     1801 ctgaaatgca ggatattaat gcttttcaga tgcgctataa cgcattcttt cagcctgccg
     1861 agggcgttca ctggctgcat taaaaccgct tcgtcttatc agcctacaga ccgtgcctga
     1921 cctgtcgagt ggataaggcg ttcacgccgc gtccggtatc ggaactgaca gtaggtgaac
     1981 gcattatcta cgttctattt acagcagcac atccagcccg tgtttttgca caatgctgag
     2041 cagttttaat gaaatgccat tggggcgttt agcgccactt tcccattttt gtacagttga
     2101 aacgctggta ttgagatagt gagcgaagac gggctgactc acttttaatt tttcccgcag
     2161 tgcttttatc tcaacaggtt gtaagtcttg cacgctggtc aggcaggctt tgtcaaaagt
     2221 acgcattgtt tcggcagaaa tagcatcgac gctggacaat ccagccgctg cgctgtgaat
     2281 agcctcaaat gcaggacttt taaatttagt ttttgcagtc atagcagatc tctgtcagtt
     2341 ctttagtgtt aatcattgct gttagctggg ctttcgtcag gaaggcataa tgctttgcaa
     2401 gttcacgaaa cccagccagt tcctgactat tgatgttcga catatcctgt tttgcatata
     2461 aaaacgtgta aaaccaattg cttccccctt ttgccagaat aattgcacgc tcacgatttt
     2521 ggtgaaggcg ctttttaaaa acgccaccac caagtgcatc agctttacct tgctccgttt
     2581 ctaaaatggc ccggcacaac tcttcatctg ttattgcatg agacctggct tcccttgcaa
     2641 accatttggt tttaaacacg cgcatatcgt tgaggtcctg ttattaaagt atatcaccaa
     2701 gtgcaatact ttatccctta ttcccaaccc aatcgcgaaa atgcatatca gcttgcccta
     2761 tgatttttgt ttcttcgagc agcctcgaac ttttcccaca acaccgtaaa agttgcaaaa
     2821 acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg cttttcccct gaaacatgcc
     2881 acgggtaaca caatgagtga atttattgct gaaaaccgcg gcgcggatgc catcacccga
     2941 ccgaactggt cagccgtttt ctcggtggcg ttttgtgtcg cctgtctgat tatcgttgag
     3001 tttttgcccg tcagtttgtt gacgccaatg gcccaggatt taggcatttc ggaaggggtt
     3061 gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt ttgccagttt gtttattacc
     3121 cagacaattc aggccactga ccgccgctac gttgttattt tatttgccgt tttgctgacg
     3181 ctctcctgtt tgctggtttc ctttgctaac tcattcagtt tgcttttaat cggtcgtgcc
     3241 tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg cgtcgctgac catgcgtctg
     3301 gtaccgccgc gtacggtgcc gaaggcgctg tcggtgatct tcggcgcggt ttctattgcg
     3361 ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc ttatcggttg gcgcaatgtc
     3421 tttaatgcgg cggcggtgat gggcgtgctg tgtattttct ggattatcaa atcattgcct
     3481 tcactgccag gcgaaccctc gcatcagaaa caaaatactt tccgcttatt acaacgtccg
     3541 ggtgtgatgg cagggatgat cgccatcttc atgtctttcg ccggacagtt tgctttcttc
     3601 acatatattc gtccagtgta tatgaacctg gcgggattcg gtgtggatgg cttaacgctg
     3661 gtgctgttaa gttttggtat cgccagcttt atcggtacgt cgctttcgtc gttcattctt
     3721 aaacgttcgg taaaactggc cttagcaggc gcgccgttaa tactggctgt gagtgcgttg
     3781 gtgctgacgt tgtgtggaag cgataaaatc gttgctaccg gcgtggcgat tatctggggg
     3841 ctaacttttg cattggttcc cgtcggctgg tcaacgtgga tcacccgctc actggccgat
     3901 caggcagaaa aagccgggtc tattcaggtg gcggttattc agcttgctaa tacctgtggc
     3961 gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt gatgttgtcc
     4021 ggcacattga tgttgctgac ggcgttatta gtcaccgcaa aggtgaaaat gaagtaacgt
     4081 atttacagga gggttatttc tctggcggga aagctatcct gttcttcccc ctgataacgc
     4141 ttgatcaggt attgttctta tcttgttgtt atttccatta ttccgcctgt attcatcatt
     4201 tgctaatcat gaatcatcat tttccctcat gttaaagaaa ggtcatttaa tctgaacgca
     4261 tagcgacagg gatgacaaat ggcagggaaa gcgcatggga atggcgatcg tcggggcgat
     4321 aacaccatat gtggattggg ggatcgttta cgtcgtctga ccgccggtat ttgcctgata
     4381 acacaaacta tattccctgt tatggccgct gcccctacgc atattaatca tgcgcattcg
     4441 gatactacca cgtccctgat cctgccgaac gttaaaacta ttccatatac cctgggggca
     4501 ttggaatcgc catcaacggt cgcggcacgc tttggtatca ccgttgatga gttacgtcgc
     4561 ctgaaccagt ttcgtacctt tgcgcggggc tttgacaatg tgcgtcaggg cgatgaaata
     4621 gatgtgcccc tcattaacag taatagccct gaagcgcgca acctgaaagc aatgcagatg
     4681 gagcgcgacg gcaaagatcc gcaaatgcag gtcgcggaaa tggcgcagca aagcggaaca
     4741 cttttagctc gcgatatgga cagcgaacag gcggcgagta tggcgcgtgg ctgggtggct
     4801 tcttctgcat ctgcgcaggc aacggactgg ctcagtcgtt ggggaactgc ccgggtatct
     4861 cttggtgtgg atgaagattt cagccttaaa agctcttcat tcgaatttct ccatccctgg
     4921 tacgaaacgc cggataacct ggtcttcagc cagcacacac ttcatcgtac ggatgatcgc
     4981 actcaaacca accacggtat cggctggcgt tatttcaccc cgagctggat gtctggcgtc
     5041 aatatgttta tcgattatga cctgacccgc tatcacaccc gaaccgggat gggcgttgaa
     5101 tactggcgcg attacctgaa actgagcggc aacggttatc tgcgcctgag taactggcgt
     5161 agtgcaccgg aactcgacca tgactatgaa gcacgtccgg cgaacgggtg ggatcttcgc
     5221 gcggaaggct ggttaccggc ctggccgcag ctgggcggca agttggtcta tgaacaatat
     5281 tatggcgatg aagtggcgct gtttggtaaa gacgagcgac aaaatgatcc tcacgcgatt
     5341 accgcaggtt tgagttacac ccctgttcct ctgatctcct ttagtgccga gcaacggcag
     5401 ggcaaacagg gagaaaacga cacccgaatt ggtatggagt tgacgttgca acctggtcat
     5461 tcactgcaaa aacagctcga tccggctgaa gttgcagcac gacgcagcct tgtgggcagt
     5521 cgctatgacc tggtcgatcg taataacaat attgtcctcg aatatcgcaa gaaagagctg
     5581 gtgcggctga cgctgaccga tccgttgaaa ggcaagccgg gtgaggttaa atcgctggtt
     5641 tcttccttac aaaccaaata tgcgctgaaa ggttatgaca ttgaagccgc ctccctgcaa
     5701 tcagcgggcg gaaaagtagc tgtttccggc aaggatattc aggtcactat tcctccctac
     5761 cgattcacgg caatgcctga aaccgacaac acttacccga tagcggttac agcggaagat
     5821 tcgaaaggaa atttctcccg gcgtgaagaa agtatggtgg tcgtggaaga acccacacta
     5881 agcctcgccg attcaacgct ttctgtcgat ctgcaaatcc tgcttgctga tggaaaatca
     5941 acctcaacgc tgacctatac cgcgcgagat tccagcggca agccaatccc cggtatgacg
     6001 ttaaaaacac aggcgaaagg tttgcaggat ttcgccttaa gcgaatggaa agacaacggt
     6061 aacggaacct atacccaaat agttaccgcc gggaagacgt ctggcgcgtt atcgctgatg
     6121 ccgcaattta acggcgacga tatcgctaag acgccagcgc tgatagccat tgtggcgaat
     6181 acggcatctc gtgctgactc cacgattgag acagaccagg ataattacgt cgcgggtaag
     6241 ccgatagtcg ttaaagtgac cctgagagat gataacgaca acggggttac cggacgaaaa
     6301 gaacttctca aacaaaccgt taaagtagac aacacgaagg ctgatgccgt tagtgcctgg
     6361 acggaagaaa gtgagggaat ttacaaagca agctatacgg cccatctcat tggcgacaaa
     6421 ttaacggccc agctgacgat gcccggctgg caaaccaaac attcggatgc gttcagtatt
     6481 gcgggtgata aagataccgc caaaatcgct gcgatgcaga tcaccgcgaa taacgcagtt
     6541 gccagacgcg atcacaatac cgtcgctgtg acggtcaggg atgtgcatca aaatttactg
     6601 cagggacaaa atgttacctt cacggtcgtg aatggtacgg cggtttttgc cgatccgaat
     6661 ggcggtattg tgacgacaga taaagacggg atagccagcg ttaaccttgc cagtgaccag
     6721 gcggtaaaca gtcttatcaa ggccgagatc aatggcagta gtcagtctgt ggaagtgagc
     6781 tttatcactg gcgatatctc gcagctgacc tccaccatca agacggacga tgtgtcatac
     6841 actgcgggtg gcaagataaa agtgtccgtc acccttatgg atgagcagaa aaacctggtg
     6901 aaagggatgg cttcgctgct ggcgggtagc ggtgtggtcg aggtgagcgg tacagataaa
     6961 aatgaaacgg gtaactggag cgaagagtct gacggcgttt acaccaccac ccgaaccgcg
     7021 aagattgctg gcgatcgcca ttacgccacg ctgaaactat cgacatggag tagcgcgcag
     7081 caatccgatg cgtatgccat ccgcgaaagc ggagctgtgc tggcttattc ctccatcgtt
     7141 accgacaaaa cgacctacac cgcgggcggc gtgataaaag ttacggtaac actcaaggat
     7201 agctatgaaa acctggtcgg ggggcaacga gacgctatca accaggctat tcagctgcca
     7261 aatacaaaag cggaaagcat cgcctggaat gaagatcaaa aaggaattta taccgcaacg
     7321 tataccgcct tactccctgg caccggttta aaagcacagc tacagatgtc cgactgggcg
     7381 agcgctctga cttcaaatga ttacagtatt agtggcgatg ccgccagcgc acaaattgtc
     7441 gcgatgcaag tgacaacggg taacccggat gtgctggcga atggcagcga tcgacatacg
     7501 gtgaatgtcc gggtggaaga tcagtttggc aatgtgctgc cggagcagac cgtcaccttt
     7561 acggtgacga agggggcggc tgtatttgcc aatgcagggc agagcgcaga tatcaggacg
     7621 gatgcgcatg gtatggcaga ggttgatctg agcagtaccg tagcggatgc cagcactgtt
     7681 gaggctaagg tcaatcaaag cagcgatagt aaaacggtga actttgttgc tgatgttagg
     7741 acggcgcaga tcgctgatct ggtggtcatt aaggatggct cagtggcaga cggttcgacg
     7801 gcgaatacgc tgcgggtgag ggtgtcggat gcgttcggta atgcgctgga cgggcagacg
     7861 gtcagcgtgt tggcggacaa tggcgcaacg gtcgctccga cggttatcac cgagccggac
     7921 gggacggtgg agatctctgt taccagccag acggcgggag tcagtgcggt caccgccagt
     7981 atcaacaaca gcagcctgag ccggaacgtg acgtttgttg ccgatgtcag gacggcgaag
     8041 atcgctgatc tggtggtgat gcaggacggc tcagtggcag atggcacgac ggcgaacacg
     8101 ctgcgggcga gggtgaccga tgcgttcggc aatgcgcttg ccgggcagac ggtcagcgtg
     8161 actgcaggca acagcgcaac ggtcgcttcg acagtgacca caaagccgga cggcacggtg
     8221 gagatcagtg tcaccagcca gacggcggga gtcagtactg tcaccgccag catcaacagc
     8281 agcagtcaga gccgggatgt gacgtttgtc gc
//
LOCUS       DAZGBC010000188         8143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGBC010000188 DAZGBC010000000
VERSION     DAZGBC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.189"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..284
                     /gene="ecpR"
                     /locus_tag="ACHK1J_004346"
                     /gene_synonym="matA"
     CDS             <1..284
                     /gene="ecpR"
                     /locus_tag="ACHK1J_004346"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO7913535.1"
                     /translation="YWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKITD
                     REMEIIRMTAQGMQPKSIARIENCSVKTVYTHRRNAEAKLYSKIYKLVQ"
     gene            359..946
                     /gene="ecpA"
                     /locus_tag="ACHK1J_004347"
                     /gene_synonym="matB"
     CDS             359..946
                     /gene="ecpA"
                     /locus_tag="ACHK1J_004347"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO7913536.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1004..1672
                     /gene="ecpB"
                     /locus_tag="ACHK1J_004348"
                     /gene_synonym="yagY"
     CDS             1004..1672
                     /gene="ecpB"
                     /locus_tag="ACHK1J_004348"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO7913537.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1698..4223
                     /gene="ecpC"
                     /locus_tag="ACHK1J_004349"
                     /gene_synonym="yagX"
     CDS             1698..4223
                     /gene="ecpC"
                     /locus_tag="ACHK1J_004349"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO7913538.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSMWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4213..5856
                     /gene="ecpD"
                     /locus_tag="ACHK1J_004350"
     CDS             4213..5856
                     /gene="ecpD"
                     /locus_tag="ACHK1J_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO7913539.1"
                     /translation="MRVNLLITMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5825..6535
                     /gene="ecpE"
                     /locus_tag="ACHK1J_004351"
     CDS             5825..6535
                     /gene="ecpE"
                     /locus_tag="ACHK1J_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO7913540.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6848..7177
                     /locus_tag="ACHK1J_004352"
     CDS             6848..7177
                     /locus_tag="ACHK1J_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7913541.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7388..8002)
                     /locus_tag="ACHK1J_004353"
     CDS             complement(7388..8002)
                     /locus_tag="ACHK1J_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO7913542.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         2143 a         2106 c         2079 g         1815 t
ORIGIN      
        1 attattggta ttacaacagc aatattagag gcgtggtata cgctggactg agtcgtgata
       61 ttagaaaaga actggcctat gtgattaatg gcaggttcct gagaaaagat attaagaaag
      121 ataaaatcac tgaccgggaa atggaaatta tccgcatgac ggcccaggga atgcaaccta
      181 aatcgattgc cagaattgaa aattgtagtg tgaagacagt gtatacccat cggcgtaatg
      241 ctgaggccaa gctgtactca aaaatatata agttggttca gtaaactcca ggcaagttag
      301 ttttaaaaaa tgactcactg ggacatcacg tcctcaattc aactcgggaa gaaatgcaat
      361 gaaaaaaaag gttctggcaa tagctctggt aacggtgttt accggcatgg gtgtggcgca
      421 ggctgctgac gtaacagctc aggctgtagc gacctggtcg gcaacagcca aaaaagacac
      481 caccagtaag ctggttgtga cgccactcgg tagcctggcg ttccagtatg ccgaaggcat
      541 taaaggtttt aactcacaga aaggtctatt tgacgtggct atcgagggtg actcaacggc
      601 taccgccttt aaactgacct cacgtcttat caccaacaca ttaacccagt tggatacctc
      661 aggttccaca ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa aaactggcga
      721 taccgtgatg atcgataccg ccaacggcgt actgggcggc aaccttagcc cgctggctaa
      781 cggttacaat gccagcaatc gtaccaccgc acaggatggt ttcaccttca ccatcatcag
      841 cggcaccacc aatggtacca ccgcagtaac cgactacagc actctaccgg aaggcatctg
      901 gagcggcgac gttagcgtac agttcgacgc gacctggacc agttaatctc tctgatgtac
      961 cagcaggggt agcccccctg ctttattcct ggacggaatt gttatgaaaa agcaccttct
     1021 gcctctcgct ctgctgtttt ccggaatatc tccggcccag gcgctggatg tcggcgatat
     1081 atcatcgttt atgaacagtg acagcagcac gctgagcaaa acgatcaaaa acagtaccga
     1141 cagtggtcgc cttatcaata tccgtctcga acggctctct tcaccgcttg acgacgggca
     1201 ggttatctca atggacaagc cggatgagtt gctactcact cccgccagct tgctgctacc
     1261 cgcccaagcc agcgaagtga tccgcttctt ctataaggga cccgcagatg aaaaagagcg
     1321 ctactaccgc attgtctggt ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa
     1381 ccgcagcgct gtggccactg cttccgcccg catcggcacc attctggtcg tcgcccctcg
     1441 ccaggcgaac taccactttc agtacgccaa cggctccctg acaaatacag gaaatgcgac
     1501 gctgcggatc ctcgcctacg gcccttgcct gaaagccgcc aacggtaagg agtgtaaaga
     1561 gaattactac ctgatgccgg gcaagtcgcg tcgttttacc cgcgtggaca ctgcggataa
     1621 caaaggacgg gttgcacttt ggcagggtga taagttcatt cccgtgaaat agatagctgt
     1681 gcagatggat aacgacaatg cctttacgac ggttctcccc aggactgaaa gcccagtttg
     1741 ccttcggcat ggtctttttg ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc
     1801 agcaaatagg tggggtgatt attccgcagg ccttcagtca ggcgcttcag gacggcatga
     1861 gcgtcccgct ctatattcat ctcgccggta gccagggtcg ccaggacgat cagcgaatcg
     1921 gcagcgcttt tatctggctg gatgatggac agctacgcat ccggaaaata cagctggaag
     1981 agagtgaaga taacgccagt gtcagcgaac aaactcgaca gcagctgatg gctctggcga
     2041 acgccccgtt caatgaggcc cttaccatcc ccctgactga caacgcgcag ctggatctca
     2101 gcttgcgcca actgctgctg cagctggtgg tcaagcgcga agcgctgggc accgtactac
     2161 gctcacgtag cgaagacatc gggcagtcca gtgttaacac cctcagcagt aatctgagct
     2221 ataacttggg cgtctataac aaccagttgc gtaacggcgg gagcaacaca tccagctatc
     2281 tgtcgctgaa taacgttact gcgctgcgcg aacatcatgt ggtgctcgac ggctcgctgt
     2341 acgggatcgg tagcggtcaa caggacagtg aattatataa agcgatgtat gaacgcgatt
     2401 ttgccggtca ccgatttgcc ggtggaatgc tcgacacctg gaacttgcag tccttagggc
     2461 cgatgaccgc catttcagca gggaagattt acggcctttc ctggggaaac caggccagct
     2521 ccaccatctt cgacagcagc cagtcagcca cgccagtgat cgccttttta ccggcggcgg
     2581 gtgaagtaca tctcacccgt gatgggcggc tactaagcgt tcagaacttc actatgggca
     2641 atcatgaagt ggatacccgg ggtctaccgt acggtattta cgatgtggaa gttgaggtga
     2701 tcgttaacgg tcgcgtgatc agcaaacgca cccagcgggt caataagctg tttagccggg
     2761 ggcgcggcgt tggtgcacca ctggcgtggc aggtatgggg cggtagcttt catatggatc
     2821 gctggtcgga aaacgggaaa aagacgcgac cagctaagga gagctggcta gcaggtgcct
     2881 cgacctccgg ctcattgagt acgcttagct gggcggcaac gggatatgga tacgataatc
     2941 aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg agcgatcaac gttaacctgc
     3001 aaaatatgct ggccagtgac agctcatgga gcaacatcgc cagcatcagc gccactctac
     3061 cgggaggctt tagttcgatg tgggttaacc aggaaaaaac ccgcattggc aatcaattgc
     3121 gacgtagcga tgccgacaac cgtgcaatcg gcggcacact caacctgaac tcactgtggt
     3181 cgaagctggg cacattcagc atcagctaca atgatgaccg ccgttacaac agccattatt
     3241 acacagcaga ttactatcaa aatgtctaca gcggtccctt tggttcgctt ggcctgcggg
     3301 ccggtattca gcgctataac aacggcgaca gcaacgccaa tacagggaaa tatatcgctc
     3361 tcgatctctc gctaccactg ggcaactggt ttagcgcagg gatgactcat caaaacggct
     3421 acaccatggc aaacctgtca gcacgcaagc agtttgatga agggaccatt cgcactgttg
     3481 gtgccaatct gtctcgagcc atctccggcg ataccggtga tgacaaaact ctcagtggtg
     3541 gggcgtatgc acagttcgac gctcgctacg ccagcggaac gctgaacgtc aatagcgcgg
     3601 cggacggcta cgtcaatacc aatttaaccg ccaatggcag cgtcggctgg cagggtaaaa
     3661 acattgctgc cagcgggcgg actgatggca acgctggggt gatattcaac accgggctgg
     3721 aggacgacgg tcagatcagc gccaaaatca acgggcggat tttcccactt aacggcaagc
     3781 gtaactatct cccactctct ccctatggaa gatatgaggt ggagttacag aacagcaaaa
     3841 actcactcga cagttacgat atcgtcagcg gccgcaaaag ccatctgact ctctatccag
     3901 gcaatgtcgc tgtcattgag ccagaggtga agcagatggt taccgtctcc ggtcgtatcc
     3961 gtgcggaaga cggcacactg ctggctaacg cacggattaa caaccatatc ggccgaaccc
     4021 gaaccgatga aaacggcgag tttgtcatgg acgtggataa gaaatacccc actatcgatt
     4081 ttcgctacag tggcaataaa acctgcgaag tggctctgga actcaaccag gcgcgcggcg
     4141 ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc gtgggcggcg gtgacgcaga
     4201 caggagaaga gaatgagagt taacctacta ataacgatga taatctttgc gctaatctgg
     4261 cctgcaaccg cgctcagagc tgcagtgagc aaaacaacct gggcggatgc accggcacgc
     4321 gagtttgtgt ttgtcgaaaa caactcagac gacaactttt tcgtcactcc tggcggggcg
     4381 ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt taaaatacaa tggttcagga
     4441 accatctatc agcaaagcct cggctacatt gataacggtt acaacaccgg cctctatacc
     4501 aactggaagt ttgatatgtg gctggaaaat tcgccggttt catctccttt aactggctta
     4561 cgctgcatca actggtacgc agggtgtaat atgaccacca gtcttatcct gccgcaaacc
     4621 accgacgcca gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa gtggatgcac
     4681 ggcatgttgt cagacgcgtt ttaccagtat ctgcaacaaa tgcccgtcgg cagcagcttt
     4741 acaatgacca tcaatgcctg ccagacctct gtgaactatg acgccagcag cggcgcacgc
     4801 tgtaaggatc aggcctccgg caactggtat gttcgcaacg tcacccatac gaaagcagca
     4861 aatctacggt tgataaatac ccactcgctg gcggaagtat ttatcaacag cgacggagta
     4921 ccgactctgg gcgaagggaa cgccgactgc cggacgcaaa ccatcggcag ccgttcagga
     4981 ttaagttgta agatggttaa ctataccctg caaacaaacg gactcagcaa cacctcaatc
     5041 catatattcc cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg
     5101 cagttcagtc tgaatggcag ttcatggaaa ccggtgagca ataccgccta ttactacacc
     5161 ttcaacgaga tgaagagcgc agactcgatc tatgttttct tctcgagcaa cttctttaag
     5221 cagatggtga acctcgggat cagcgatatc aacaccaaag atctattcaa ctttcgcttt
     5281 cagaacacca catcaccgga gtctggctgg tatgaatttt ctacctccaa cacgctgatt
     5341 atcaaacccc gtgatttcag catcagtatt atctccgatg aatatactca gacaccgtcg
     5401 cgggagggat atgttggcag cggcgagtcg gcactcgatt tcggctatat cgtaaccacc
     5461 agcggtaaaa cagctgccga cgaagtgctg atcaaggtga ccggacccgc gcaggtgatt
     5521 ggcgggcgct cctattgtgt cttcagctcc gatgacggta aggcgaaagt accgttcccg
     5581 gcgacacttt cctttattac ccgcaacgga gctacaaaaa cctacgatgc cgggtgcgat
     5641 gatagctggc gggatatgac cgatgcgctg tggttgacca caccgtggac tgatatctct
     5701 ggcgaagtgg ggcagatgga taagaccaca gtcaaatttt cgattccaat ggataacgcc
     5761 atttcgctgc gtacggtaga tgataacggc tggtttggcg aagtcagcgc ttcaggagag
     5821 attcatgttc aggcgacgtg gcgtaacatt aactaaggcc ctgctgacag cggtctgtat
     5881 gctggcggca cctttgacac aggcgatttc ggtcggcaat ctgacatttt cgctgccgtc
     5941 cgagactgac tttgtcagca aacgtgtagt gaataacaac aaaagcgcac ggatataccg
     6001 tattgccatc agtgctattg atagcccggg cagcagtgaa ttgcgcaccc gaccggtgga
     6061 tggtgaactg cttttcgccc cccgccagct ggcgttgcag gctggtgaga gcgagtattt
     6121 taaattttac tatcatggtc cacaggataa ccgcgagcgc tactaccggg tctcatttcg
     6181 cgaggtcccc actcgtaacc agacaagacg cagcccaacc ggcggcgagg tcagcacgga
     6241 gccggtggtg gtgatggata ccattctggt agtacgacca cgtcaggttc agtttaaatg
     6301 gtccttcgat caggtgaccg gaacggtaag taacaccggc aacacgtggt ttaagctact
     6361 gattaagcca ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc tacgtccggg
     6421 agacgtggtt catcagcctg agttacgtca gccggggaat cattatctgg tctataacga
     6481 caaattcatt aagattagcg attcctgtcc ggctaagccc ccttcggcgg actgatactt
     6541 atccatcggt cgagggaaga attccacctc agagactcaa aaaacgttta aatgatgtgg
     6601 aagcgatcgt caatgtcacc tgtaacgatg ggctttctca tatgcacacc atagtgttca
     6661 ggagacacag aaaggtatca ggctgcctgc taacgagtgg tttattcacg aaatagtacg
     6721 taaaatcggt aacggctgga aatcattcaa tactcacact atcgaaagta caccagccaa
     6781 ccgcggtacg ttcttacata cgatgtaccg ctgttctctt tacgatttat agctgtactg
     6841 gtgaattatg agcaatctga atccatgcat gacgtgtggt gcctgttgtg catttttccg
     6901 cgtctctttt tactgggccg aagccgacga tgctggcggt accattcccg ccaggctcac
     6961 tgaacaaata tccccttttc accgatgcat gagcggtacc aatcagaaaa acccccgatg
     7021 tattgccctt gcaggaaccc caggcaaaaa tgcctgctgc acgatatata aaaatcgatc
     7081 gtccacgtgc agagaattcg ccatgtctgg tgagaacgga gaagtcaatg aggcttgcaa
     7141 tcgtgcaagg gctaaatacg ggctgacacc gttataaata tacaataatt aattgcactg
     7201 ccccgccagc gataatagcg gggcttcgtt ttcagggaca acaaaacccg aaacttcttg
     7261 tatttgccag caacaatgcc tctcttctgt agcgttcttc ggatctacat catcctaaag
     7321 tagcgcgata tactcactga ctatctgaat cagaatataa aaagcaatgt ttttatcatt
     7381 caatgcatta tctgtttgag cctaagggga tctcagggtc tggctcatga gtaattctgt
     7441 ttcgtaaatc tctgcgaata atttcaatgg accagaacca gactaaatgt ccaaaaattt
     7501 cagaaacatt ctcataccac gggagatcaa aaagaggtgg tgtcagtccc atgagaggga
     7561 atgaaatcat atgaacaaaa agttgggcta aagcacctgc cagtaagccc tgccagagtt
     7621 taatttttgg aaatacttca gcgaccacac aataaccgac agcaaacact atcgaaaaga
     7681 taatgtgcgt aacaccaacc cagttaaaga catgcccggc aaaggtataa acagccgcat
     7741 tgggatctgt cagccccagc cagtctcgaa gaaaaatata cggaggattg agaaaattac
     7801 gcgagcaatc aatttgccca gcagccctga ttaatgattc cgggccacac gctgcattaa
     7861 acatatccac cgggctacgt ggcggcaatg gaacttcagc cccccacttc acaaatgcgg
     7921 aaacaacgcc agcaatcagc ccaatgaatg cagcaagacc ataacgtctg cggttcggtg
     7981 gagtttgttc aaatatattc atatctaccc tgcttgtacc attatgttat acacctcttt
     8041 aggagtattc ataaaacaag gtaaatgtaa agaactgtat tgttttgtat aacaagatgg
     8101 ttgtctaatc gccaaagaat ataagcacca tcatttctcc cta
//
LOCUS       DAZGBC010000189         8127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGBC010000189 DAZGBC010000000
VERSION     DAZGBC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.190"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(9..85)
                     /locus_tag="ACHK1J_004354"
     tRNA            complement(9..85)
                     /locus_tag="ACHK1J_004354"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Asp,seq:gtc)
     gene            complement(218..949)
                     /gene="dnaQ"
                     /locus_tag="ACHK1J_004355"
     CDS             complement(218..949)
                     /gene="dnaQ"
                     /locus_tag="ACHK1J_004355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO7913543.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1014..1481
                     /gene="rnhA"
                     /locus_tag="ACHK1J_004356"
     CDS             1014..1481
                     /gene="rnhA"
                     /locus_tag="ACHK1J_004356"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO7913544.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1478..2200)
                     /locus_tag="ACHK1J_004357"
     CDS             complement(1478..2200)
                     /locus_tag="ACHK1J_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7913545.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2234..2989
                     /gene="gloB"
                     /locus_tag="ACHK1J_004358"
     CDS             2234..2989
                     /gene="gloB"
                     /locus_tag="ACHK1J_004358"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO7913546.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3061..4419
                     /gene="mltD"
                     /locus_tag="ACHK1J_004359"
     CDS             3061..4419
                     /gene="mltD"
                     /locus_tag="ACHK1J_004359"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO7913547.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4467..5090)
                     /locus_tag="ACHK1J_004360"
     CDS             complement(4467..5090)
                     /locus_tag="ACHK1J_004360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7913548.1"
                     /translation="MSGLPQGRPTFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKN
                     ITTRHGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQTVEALRDTSHVRNYASCEWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQNDGSFTSDIIMVEAHKAA"
     gene            complement(5094..5894)
                     /locus_tag="ACHK1J_004361"
     CDS             complement(5094..5894)
                     /locus_tag="ACHK1J_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7913549.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6135..7049
                     /gene="yafC"
                     /locus_tag="ACHK1J_004362"
     CDS             6135..7049
                     /gene="yafC"
                     /locus_tag="ACHK1J_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO7913550.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGALTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7046..7849)
                     /gene="dkgB"
                     /locus_tag="ACHK1J_004363"
     CDS             complement(7046..7849)
                     /gene="dkgB"
                     /locus_tag="ACHK1J_004363"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO7913551.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVSRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8012..8088)
                     /locus_tag="ACHK1J_004364"
     tRNA            complement(8012..8088)
                     /locus_tag="ACHK1J_004364"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8052..8054),aa:Asp,seq:gtc)
BASE COUNT         2166 a         1957 c         1982 g         2022 t
ORIGIN      
        1 gtgaatagtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta
       61 ttctaaccga ctgaactacc gctccgcgtt gtgttccgtt gggaacgagg cgaatattac
      121 ggattgcctc acccttcgtc aacggttttt ctcatctttt gaatcatttg ctgcaaaaat
      181 cgcccaagtc gctattttta gcgcctttta catgtattta tgcccgccag aggcagcttc
      241 cgcccttttt ctgcaccaga tcgagacggg cttcatgtgc tgcaagctct tcatctgtcg
      301 caaaaacaac gcgtaactta cttgcctgac gtacaaggcg ctggattgtt gcttcacctt
      361 gttgctgttg tgtctctcct tccatcgcaa aagccatcga cgtttgacca ccggtcatcg
      421 ccagataaac ttccgcaagg atctgggcat cgagtaatgc cccgtgcagc gttcgtttac
      481 tgttatctat ttcgtagcga gcacataacg catcgaggct gttgcgctta ccgggaaaca
      541 ttttcctcgc caccgcaagg ctatcggtga ccttacagaa ggtattggtc ttcggaatat
      601 cgcgcttaag caacgaaaac tcgtagtcca taaagccgat atcgaacgct gcgttatgga
      661 tcaccaactc cgcgccgcgg atatagtcca tgaactcatc ggctacttcg gcaaacgtgg
      721 gcttatcgag caaaaattca tcggcaatac catgtacacc aaaggcttcc ggatccacca
      781 gccgatcggg tttgagataa acatggaagt tattgcccgt caggcgacgg ttcaccactt
      841 caacggcacc aatctcaatg atcttgtggc cttcatagtg cgcaccaatc tggttcatac
      901 cggtggtttc ggtatcgaga acgatctggc gtgtaattgc agtgctcata gcggtcattt
      961 atgtcagact tgtcgtttta cagttcgatt caattacagg aagtctacca gagatgctta
     1021 aacaggtaga aattttcacc gatggttcgt gtctgggtaa tcctggacct gggggttacg
     1081 gcgctatttt acgctatcgc ggacgcgaga aaacctttag cgcaggctac acccgcacca
     1141 ccaacaaccg tatggagttg atggccgcta ttgtcgcgct ggaggcgtta aaagaacatt
     1201 gcgaagtcat tttgagtacc gacagccagt atgtccgcca gggtatcacc cagtggatcc
     1261 ataactggaa aaaacgtggc tggaaaacag cagacaaaaa accagttaaa aatgtcgatc
     1321 tctggcaacg tcttgatgct gcgctggggc agcatcaaat caaatgggaa tgggttaaag
     1381 gccatgccgg acacccggaa aacgaacgct gtgatgaact agctcgtgcc gcggcgatga
     1441 atcccacact ggaagatacg ggctaccaag tcgaagttta agcctgtggt ttacgacatt
     1501 gccgggtggc tccaaccgcc tggcgaattc gtggcttgtt tttactctgt ttcatcggat
     1561 ttagcgttaa aggaatagtc cgtttccggg caacaataag ttgtaagcaa ccaagcgcag
     1621 gaatatgcgc attcaaaagt tttcctccgt gtttgttcca cgggagaacg tggaaacggc
     1681 tggcgtgtag cacttcaaaa ttcaacaaag agagccagtc cagctgccgc atcagagtaa
     1741 acatccggct gttatagggc gaggttttgc gcaataccgg cacaagtttg cgtaatccca
     1801 ttaaactaat gggattgaag ccactaatca ccagccagcc atcatcaatc aatacccgat
     1861 cggcttcacg caataaacga tgcggatcgg tgcaccacgg caatgtatgt gccagtagac
     1921 aaacatcaac ggatttatcg gcaaaaggaa gatgaagtgg atccgcctgc acctggacgg
     1981 gcattccttg cgcagaaaca ttcacttgat gagaaaccgc gcacgcttcg caattgattt
     2041 ctgcgcttaa attgccaatc ttaagcagat gaaaaccata cattttagtg aaccacgggt
     2101 tgagctggcg ctccagcgcc ttgcgataaa gctctcccca gggcaaatcg ccccagcgat
     2161 caggagccac gacagtttga gggactcttg ccggtttcat cacaaccttc cgtttcacac
     2221 tgagaggtaa tctatgaatc ttaacagtat tcccgccttt gatgacaatt acatctgggt
     2281 tttgaatgat gaagcaggtc gctgcctgat tgtcgatccc ggagacgcag agccagtatt
     2341 aaacgccatt gccgccaata actggcaacc ggaggccata tttctcaccc accatcatca
     2401 cgatcacgtt ggcggcgtaa aagaactggt ggaaaagttt ccgcaaattg tggtgtatgg
     2461 tccacaagag acacaagata agggaacaac acaggtagtc aaagatggcg aaactgcctt
     2521 cgttttgggg catgaattta gtgtaattgc cacaccgggt cacactttag gacatatctg
     2581 ttacttcagt aaaccttatc tattttgcgg cgacacgctg ttttctggtg ggtgtggtcg
     2641 gttgtttgaa gggacagcat cacaaatgta tcaatcactt aaaaagttaa gtgcgttacc
     2701 tgacgataca ttggtatgtt gtgctcatga atatacgtta tcaaatatga agtttgcttt
     2761 gagtattctt ccgcacgatt tgtccataaa tgattattat cgtaaagtta aggagttacg
     2821 ggcaaaaaat caaataacac tacccgtaat tctgaaaaat gagcggcaaa ttaatgtttt
     2881 tttaagaaca gaagatattg atttaattaa tgtaattaat gaagaaacat tattgcaaca
     2941 acctgaagaa cgttttgcat ggttaaggtc aaagaaagat aggttctgat aaaactttct
     3001 tgtcatcggc tccgttcgcc gttatgatcg gtcgtctttt aagcaactat tgacacacac
     3061 atgaaggcaa aagcgatatt actcgcctct gtcctgctcg tgggttgcca gagtaccggc
     3121 aacgttcaac agcacgcaca gagcctttct gcagctggtc aaggggaagc agcaaagttt
     3181 acaagtcagg cacgatggat ggacgatggg acgtctatcg cgccagatgg tgacttgtgg
     3241 gctttcattg gcgacgagct aaagatggga attccggaaa atgaccggat tcgcgaacag
     3301 aaacagaaat atttacgcaa taagagctat ctccacgatg taactttacg ggcagagccg
     3361 tatatgtact ggatagccgg gcaagttaaa aaacgtaaca tgcctatgga actggtacta
     3421 ctacccatag tggagagcgc ttttgatcct cacgcaacgt ctggcgccaa tgccgcaggc
     3481 atctggcaga tcattccgag cacggggcgc aattatggtt tgaaacagac ccgcaattat
     3541 gatgcgcgtc gcgatgttgt tgcttcaaca actgccgcgc tgaatatgat gcagcgtctg
     3601 aacaaaatgt ttgacggcga ctggcttctg accgtagcgg cttataacag cggcgaaggt
     3661 cgggtcatga aggcaattaa aacgaacaaa gcgcgtggga aatccacgga cttctggtcg
     3721 ttaccgttgc cgcaggaaac gaagcagtac gtgcctaaaa tgctggcatt gagtgatatt
     3781 ctcaaaaaca gcaagcgtta tggcgtacgt ctgccaacga ccgatgaaag ccgtgctctg
     3841 gcgcgtgtgc acctgagcag cccggttgaa atggcgaagg ttgcagatat ggcggggatt
     3901 tccgtcagca agctgaagac attcaacgct ggcgtgaaag gctccacgct gggcgcaagt
     3961 ggtccgcaat acgtgatggt gccaaagaag catgcagatc aactgcgtga atctctggct
     4021 tcaggcgaaa ttgctgctgt acagtcgacg ctggttgccg acaatacgcc gcttaacagc
     4081 cgtgtttaca ccgtacgctc tggcgacacg ctttcaagta tcgcttcacg tctcggcgta
     4141 agcaccaaag atttgcagca gtggaacaaa ctgcgcggat ctaagctgaa gccaggccaa
     4201 agtttgacga ttggtgcagg cagtagcgca cagcgactgg caaacaacag cgatagcatt
     4261 acgtatcgtg tgcgcaaagg cgattcgctt tcaagcattg ctaaacgcca cggcgtgaac
     4321 atcaaagatg tgatgcgctg gaacagcgac actgcgaatc tgcaaccagg cgataagctg
     4381 acgttgtttg tgaaaaacaa caacatgcca gattcctgac aaaccagata ataaaaaggc
     4441 accgattccc ccggtgcctt ttttatttat gccgctttat gtgcttctac catgatgata
     4501 tcactggtga aagagccatc attctgcaag gaaaaatacg ttctcacctc tgtcgatgcg
     4561 ctctgttggt aaatgcgaat agcgtctact aacgcttctg gcgtacgcat tctcacgacc
     4621 catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat cagattggct
     4681 tcattgatta acgtcaacca ctcacagctg gcgtagtttc gtacgtgaga ggtatcgcgt
     4741 aatgcttcta ccgtctgtaa ccagatgtcg cgcactgggt ggcccggaga cattacgtcc
     4801 atcacaatca gcctaccgcc aggtttcaat atcctattca cttctcgcag tgctgcgccg
     4861 acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc gttatcggca
     4921 aatggcagac tttcggcata accatgacga gtagtgatat ttttcagttg ccgggcttcg
     4981 gcagcttgtg ccacgacatc cagcatttgg gcagataagt catacgccac caccgcgctc
     5041 acgttttgcg cagcgacaaa cgttggtctg ccctgtggca gacctgacat accttattta
     5101 tcaggcttgc cgggactgaa ttcaacgagt agcggattgt gatcggaagc gcgcgtaacc
     5161 agtacagaag cttcactgac gttcagacca cggtagaaaa caaaatcgag cgggcgacca
     5221 aacgcccgac ggcgctgatc atcggtaaaa cgcacctgac gcagcgacat ttcccgcgca
     5281 aaacgatata acgcgttcat ccttctacgg ctccaggcat tgaaatctcc cgccataatg
     5341 accgggccgc tgtggtgagc tatctgatca ccaataggaa gtaactgctt actataaaca
     5401 tccacgccca gactgaagtt gacggcgtgt atattaacca ccatcaacag gcgggtgtca
     5461 ggtaatggat agaccgtcac cagtgccgac ttcgccagac gcaaaatggg ttctcgttca
     5521 cgtaacgggc agcaatacac tggatgtgcc gccgaaaggg tcattacgcc agaaggatgt
     5581 tgtggcagca cgaaagcggg tacctgatcg gcggcaagat agttagcagt cgcaaactgt
     5641 accaactctg gcgttgtttg cgcttcctgc aataacacca gatgtgcgtc tttgccgtaa
     5701 ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt atatgttcca caccaggatc
     5761 cgaatacgct cttcggtact taacggttct ccgggtggta atgcctggcc gatgctcgca
     5821 aaagaccccg gcggtaagat cctctccgca ggttgtccgg caacatagcg catggcatag
     5881 gtgttttttc gcacttttga ctttgaaacc tctattacgt caacccttcc gcactcggga
     5941 gggttctact gttataggga ttttagctca ctctttcaac gagcaattgt atattgttat
     6001 gtaagcaagt gcttacaccg gtcagaattt tgctcattat tgagaacatt aaatcggatg
     6061 acatatcacg catcctgatg gtttaatgac aaataaattc aaaatatctc atctattttg
     6121 caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc catttttgtt tcggtcgtcg
     6181 aaagcggcag ctttagccgg gcagcggaac aattagggca agcaaactca gcggtaagcc
     6241 gggcggtgaa aaagctggag atgaaacttg gcgttagcct gcttaatcgg accacgcggc
     6301 aacttagcct gacggaagaa ggcgagcgtt attttcgtcg cgtacagtca attttgcagg
     6361 agatggcagc ggcagaatca gaaattatgg agacgcgtaa tacaccgcgt ggactgttgc
     6421 gcatcgatgc cgcaactcca gtggtgctgc actttctgat gccgttgatt aagcctttcc
     6481 gtgaacgcta tccggaagtc actttatcgc tagtctcctc cgaaacgatt attaatttga
     6541 tcgaaagaaa agtggatgtc gcgatacgcg ctggtgcgtt aacggattcc agcttacgtg
     6601 ccaggccgtt atttaacagt tatcgaaaaa ttatcgcctc ccccgattat atttcccgct
     6661 acgggaagcc agaaacgatc gacgatttaa agcaacatgt ttgcctggga ttcactgaac
     6721 ccgcttccct caatacctgg ccgatagcct gtagcgatgg acaattacat gaggtgaagt
     6781 acggtttgtc atccaatagt ggggaaacac tgaaacagct ttgcctgagt ggcaacggga
     6841 ttgcgtgttt gtccgactat atgatcgaca gagaaatcgc tcgcggagaa ttggtggagt
     6901 taatggcaga taaagtgttg ccagtggaaa tgccattcag tgcagtctat tacagcgacc
     6961 gtgcggtaag tacgcgcatc cgggctttta tcgatttcct tagcgagcat gtaaaaacag
     7021 ctcccggagg agctgtcaga gaggcttaat cccattcagg agccagacct tccgggctaa
     7081 ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt atcttcggca tcaagctgta
     7141 aattttgtgc cttaagatta ctttccaggt ttttacgttt agtagaagaa ggaattactg
     7201 agtaaccttc ccccatagcc cacgccagaa tcacttgtgc cggagtcgca ttgtgtttag
     7261 ctgcgatacg agcaataacc tcatctttca gggccttacc atacgccagc gtcatatagg
     7321 aagtaatatg gatgccgtgc tgtttagccc aggcaaccac tttacggttt tgcagataag
     7381 gagagagttc aatctggtta gtagcgatgt tttcagcgcc aacagcagca atcgcctttt
     7441 ccatcaatgg gatcgtgaag ttggaaatac cgatctcacg cgtcaaccct tcttttttgg
     7501 cttccagcag cgcctgcata aactcttcaa cagagacttc atcgtttggt gacggccagt
     7561 ggattagagt cagatcaaca taatcagtac gcaatttttg caggctctct ttcagactcg
     7621 ggatcaattt gtctttgctg agattttcaa tccagatttt agtggtgatg tagagttcat
     7681 gacgtgacac gccactttct gcaatcgcct gacctacggc agcttcgtta tcatagattt
     7741 gtgcggtatc aattgcgcga taaccaagtt caagcgccgt tttcacagat gaaataacaa
     7801 cgtcgtcttt cagacggaaa gtacctaaac caaatgcagg gatagccata atatgcctct
     7861 tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc agactgttgc ggaaaaagag
     7921 cgtaaaacgc agaggatttt tgcgatttta gcaaaaatag aaatgtagac gaaaaaaaac
     7981 ctcgagcgat aactcgaggc ttcttaataa gtggcggaac ggacgggact cgaacccgcg
     8041 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
     8101 tacaaccacc ggttttatga ccggctt
//
LOCUS       DAZGBC010000190         8067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGBC010000190 DAZGBC010000000
VERSION     DAZGBC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.191"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(172..1029)
                     /gene="murI"
                     /locus_tag="ACHK1J_004365"
     CDS             complement(172..1029)
                     /gene="murI"
                     /locus_tag="ACHK1J_004365"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO7913552.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(974..2857)
                     /gene="btuB"
                     /locus_tag="ACHK1J_004366"
     CDS             complement(974..2857)
                     /gene="btuB"
                     /locus_tag="ACHK1J_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO7913553.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     regulatory      complement(2922..3112)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3226..4326
                     /gene="trmA"
                     /locus_tag="ACHK1J_004367"
     CDS             3226..4326
                     /gene="trmA"
                     /locus_tag="ACHK1J_004367"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO7913554.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMITGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4366..4725)
                     /gene="yijD"
                     /locus_tag="ACHK1J_004368"
     CDS             complement(4366..4725)
                     /gene="yijD"
                     /locus_tag="ACHK1J_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO7913555.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4725..5375)
                     /gene="fabR"
                     /locus_tag="ACHK1J_004369"
     CDS             complement(4725..5375)
                     /gene="fabR"
                     /locus_tag="ACHK1J_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO7913556.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5479..6795)
                     /locus_tag="ACHK1J_004370"
     CDS             complement(5479..6795)
                     /locus_tag="ACHK1J_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO7913557.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYATAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6838..8004)
                     /locus_tag="ACHK1J_004371"
     CDS             complement(6838..8004)
                     /locus_tag="ACHK1J_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO7913558.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKLWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2158 a         2168 c         1855 g         1886 t
ORIGIN      
        1 gagtcaagca tttatttttg ctttctctgc cggaattctc aggagaaccc cgctgacccg
       61 gcggcgtgtt tgccgttgtt ccgtgtcagt ggtggcgcat tatagggagt tattccggcc
      121 tgacaagagg aaatttaaaa taattttccg accgcgcaac attcaaccaa atcagcctaa
      181 aactgccagt ttttcgagcg tttcgaagcc gtaacgctgt aaaacgggca ataattgttc
      241 agcttctggc gtcattgcca tacaaaaggc aatattcgca tcggcagatt ttgcatccgg
      301 ggcttcatgt tctaacaacc aagccgttcg gcgagcaatc gctgcgccag aatccaccag
      361 ccgggttccc tctggcagca cttgtaacag ttcttcttgt agtagaggga aatgggtgca
      421 acccaataca acggtatctg gcggctcttt cattcttaac cacgggcgta ggatacgttt
      481 tagtgcatcc agagaaacat cttcgccatg cagcttcgct tcagccaact caaccatctc
      541 tgccgagccc agcatttcta tctggcattc attagcgaaa cgcgcgatca gctcatgagt
      601 ataagaacgt ttaactgttc cgcgggttgc cagtaatccg acaatgccat ttgccgtcag
      661 acgtgcagca ggtttaatcg ccggaacgac accaacaacc gggaagtcga acttttcgcg
      721 taaggcagga agtgaaacgg tactggcggt gttgcaagcg accacagcca gcgcaagggg
      781 ataacgttct tgcaccgcgg tgacaattgc caccactcgc tcaacaataa acgcttcgct
      841 tttttcgcca tacgggaaag cgacgttatc gaaagcataa atgtaatgga gatccggtaa
      901 gagatgccgg atctcgtcat agaccgacaa cccaccgacg ccggagtcaa acaccagcac
      961 ggtgggacgt ggttcagaag gtgtagctgc cagacaaggt gtattcccgt cctgcagttt
     1021 ggtagccata gactgtctca taatctttgt cgaacaggtt ggctatttta ccacgaactg
     1081 tcaggtgaga ggtgaccgga tatgcaaccg caagatccca caagctcaca ccgcctaatt
     1141 taactgtatg tgatgagcca gaggtctcgt cataagcgtt gtcgtatcgt tgacctatat
     1201 attggtaaga aacgtcccat tcgaaaccat aagcttgtcc agtcaactcg tacttaacct
     1261 gctgttttgc ccgacggtac agcactttat ctgtttcatc atcgcgcggg tcaacatatt
     1321 gtaacgtcaa gcgatgttca acaggaccgg ttgtaatatt tcccgtccat tccagccctt
     1381 taatcgtggc tgatttcaca ttaatgtact ggttgacatt atttatgctt ttataatcaa
     1441 tcaggttctg aatttcataa cgatatcctg agagccgcca atccagagtc ccagttaccc
     1501 cctcaattcc aatctcccac tgtttagatt cttctggctt caaatttggg ttggctgcaa
     1561 taccaggacc ataaattgaa gcaaagcgag cagccccgta ttgctgtcca agagaaggcg
     1621 aaagaaaact ggtaccgtaa gaaagtgttg cctggtagcc atcaataaac tcccagccag
     1681 cagctgtctg ccaggtacca tgccagccaa actgttcatc atgatcttct cggccagaag
     1741 cttccaaagt cacattggaa atctgttgct gtccagtcag gtataacccg gtggtatcgc
     1801 gttcgtaatc atctgatttt gtaatgctgc tggacgtcag tttttcctgt ttccaatcga
     1861 tcccgccact aattgcacca tgccctacct caatgctatt accccactga atataacgct
     1921 gttccatgtc atccagggtg gcatctcccg cataacgacc aagattattt gtgtagttat
     1981 aattcttgag tcgttgatag ttagcaatta actgagacga atagatgccg gagttatagc
     2041 gtaggccagt atcccaggac tggttgtaat tctgagcttc ttcgttgcca gtagcagaac
     2101 catacatcga gccctggtcg taatcattat tagctgtgta gctgtaacca cggaagaacc
     2161 ccgagaagtt atcgttaaac ttatgctgca gcccccccca gaaaagtttg ttgcgatatc
     2221 catcgcgatc gctatcacca ctgtatggtg agccaggctg aatgttaaaa cctttagtcg
     2281 tctggtaagc acctgctgct gttaccatcg tatcgccaaa acgtttattt attaccccat
     2341 cataggcttg ataaccattt gtacccattc ctgcgttgat ctgagctttc tcatcattac
     2401 tcattgtaat aatgttcacc acacctccga tggcaccaga accataaacg gcagaacgcg
     2461 gcccacgaat atattcaaca cgctgtacaa gtgataccgg gaactggcta atatcaattg
     2521 cattgtcgat accggcacga gccatcggca caccgtcaat caacactaaa acatggcgcg
     2581 attcagtgcc acgaacataa agagaagaac tcgtccccat tccaccagtt tgagagatat
     2641 ttacaccggg gagatgactc ataacatcgt tgaggtcttt cgactgccag cgttgaatat
     2701 cctcacgggt aacaatatca gtaggtgcaa ggacagtatt tactggctgc tgaaaacggt
     2761 tagcagtaac gacgagagta tccgggctgg tatcctgtgc ccaagcggaa aaggctgtga
     2821 cagaacacgc cgtcagcagc gaagcttttt taatcattgt aaagcatcca caatagaaga
     2881 aggatgccgc aggtttcatc aatattacgc gatgatgaga accagatgcg acgttagccg
     2941 gcaggtcttc gggcttggag gggtatctaa gatactaaga tatgatgact tcccaccgaa
     3001 tggcagtgtc cgcataacgc aatcatcgca cctttcctta ccgctgcgcg tcagctccag
     3061 attcgcactg gattccctat taactcacag gaccggcaag tggatgctac aggttgtaac
     3121 aagttactgt ccagacgtag ctcacaaata ggaattcatc aagatctgga catctgatga
     3181 gcaatcccta caatcgccgc gtactttaat ttttcaggat acatcatgac ccccgaacac
     3241 cttccaacag aacagtatga agcgcagtta gccgaaaaag tggtacgttt gcaaagtatg
     3301 atggcaccgt tttctgacct ggttccggaa gtgtttcgct cgccggtcag tcattaccgg
     3361 atgcgtgcgg agttccgcat ctggcatgat ggcgatgacc tgtatcacat cattttcgat
     3421 caacaaacca aaagccgtat ccgcgtggat agcttccccg ccgccagtga actcatcaac
     3481 cagttgatga cggcgatgat tacgggtgtg cgtaataatc ccgttctgcg ccacaagttg
     3541 ttccagattg attacctcac cacgctgagc aatcaggcgg tcgtttccct gctataccat
     3601 aaaaagctgg atgatgagtg gcgtcaggaa gcggaagccc tgcgcgatgc actgcgagca
     3661 cagaatctga atgtgcatct gattggtcgg gcaacgaaaa ccaaaatcgc gctggatcaa
     3721 gattacatcg acgaacgtct gccggtcgca gggaaagaga tgatctaccg tcaggtagaa
     3781 aacagcttta cccagccgaa cgcggcgatg aatattcaga tgctggaatg ggcgctggac
     3841 gtaaccaaag gctcaaaagg cgatttactg gagctgtact gcggcaacgg taacttctca
     3901 ttagccctgg cgcgcaattt tgatcgggta ttagccaccg aaatcgccaa gccgtcagtc
     3961 gctgccgcgc aatacaatat tgccgctaac catattgata acgtacaaat tattcgtatg
     4021 gcggcagaag aatttactca ggcgatgaat ggcgtgcgcg agtttaaccg cctgcaaggg
     4081 atcgacttaa agagttatca gtgcgaaacc atttttgtcg acccaccgcg cagcggtctg
     4141 gacagtgaaa ccgagaaaat ggtgcaggcg tatccacgta ttctgtatat ctcctgcaat
     4201 ccggaaacgt tatgcaagaa tctggaaaca ttaagccaga cgcacaaggt cgaacgtctg
     4261 gctctgtttg atcagttccc ctacacgcac catatggagt gcggcgtatt actgaccgcg
     4321 aagtaaaacc catgccggat gcgccagcat ccggcatgat accgattact cagcaacttc
     4381 ctgcttacgg ttacgcatct tcgcgccaat ccagaacacc ataatgacgg agagtactgc
     4441 cgggaagaag ttagagccga tatccggata ttccgcacga actaccgtgc tgtacagcag
     4501 tacgccgaga ataaaacagg cagctgccag acccggcaag cctaccggca tggtgcgatt
     4561 aagataacgt tgatgcaggc agtaaaccgt cagcaccagg gaaataatcg ggaatacaga
     4621 aaatggcaca atggagctaa acagcgctgc gaaagtacca ttaatcgata agccagcaac
     4681 caacgccagc agcagcgtac ctctatcttg atttgcttgt ttcattactc gtccttcaca
     4741 tttcccggaa taattgcggt tttctcttgt tcacggcgat accagtaata agcccctttc
     4801 gaaatcattc gcagttgcag taccagtcgc tcttctaatt gccgacgttg ttcgacgccg
     4861 acgtccaacg cctcggcacc cgcactgaag acaattgtca ccattgcttc ggcttgcgct
     4921 tcagtaaacg cacgcggcat atggttttcg agttccagat agtccgcaag ttccgcaatg
     4981 aagtgctgaa tttcacgcgc aacggcggca cgaaacgcag cggaggtgcc ggagcgttcc
     5041 cgcaataata accggaaggc gttaggatta ttaccgatga actccataaa tgtggagacc
     5101 gaggtacgga tcacactccc gcctttggcg atacgctgac gcgcctggcg catgagttgg
     5161 cgtagcatta aaccgctctc atcaaccatg gtcagaccca gttcgtctac gtcgcggaaa
     5221 tgccgataaa aagaggtggg agcaatgccc gcttcacgcg ccacttcacg caaactcagg
     5281 ctggcgaagc tgcgttcagc acttaattgg ctaaatgcgg cttccaccag cgaacggcgg
     5341 gttttttctt tttgttgcgc tcttacgccc atcacgatgt ctgaatcctt gccaaaagta
     5401 ctgctggcac tataccagag aatgaacata ctggattgct ccagtattgt gaatgaacgg
     5461 taacgcaata aaacatattt actgcaattc tttggccggt tcttttacgt acaacaacgc
     5521 caacatcgac agcaacgtgc ctccacccaa ataccacgcc ggagccgtca aactatccag
     5581 caacacgatg gattgcgtag cgaaatattg agcaaaaccg ccaaagattg ccacaccaat
     5641 gctataaacc agcgcaaaac ccaatgcccg aatccgtttc ggcaacagtt cagagatcaa
     5701 caacatcact ggcacaccgc caagcgtggt aaagctgacc atcacgaaaa ccaccgtcag
     5761 caacatgcca gggcttgggt aattcaccaa cagccagaat gacggccagc tacagatcag
     5821 caccatcaca cgggagatta aaatcagctt tttacgcccg accgaatcgc acaacatccc
     5881 caccagcagc gcacccacaa acgtaataac gcccgccagt aacatcgctg catgagaata
     5941 gttttggttc attcctaaat acttcgccgc ccatgtaccg taataaaaga gggagatata
     6001 ggttgcgact gtactaccaa tagcgagcaa gataccgtta gcgatagtcg ccttatgttg
     6061 caggagcaga gaaaacgcgc tttcgctggt agcagctttt ttattgcgca caggctcagg
     6121 tacgtcattt tccagactca aacgcaacca acagccgaca ggcgctaata acacgccaat
     6181 aaaaaacggc acacgccagc cccattcagc catgacggtt tctgaaccgg tcgcaaaggg
     6241 cagccatgca cttaaaccta aagcgacaac acctccaaaa gttgttgcca ggccttgagt
     6301 cgccagtgac caactgctgt aaaaaccgcg acgatttgcc ggagctgact ccaccagtag
     6361 cgacattgaa gccccaactt caccgcctgc tgcaacaccc tgaatcaaac gagcaagtac
     6421 cagagttgcc gttccccaat agcccgccgt cgcatacgtc ggcgcaatgc caatcatcaa
     6481 cgtgccgagg ctcatcaggg caatagtgaa caccatcgcg ggtttgcgtc cgaaacgatc
     6541 ggcccaggcc ccgacgataa ttccaccaag cgggcgcata aaaaaactca cgccaaacgt
     6601 tgcaaatgcc agtaacagcg cgacattgtg atcagcgctg ggtgggaaga aaagtgtact
     6661 gatatagacc acaaaaaagt tgtaaacgcc aaaatcaaaa aattcgagcg cattgccaaa
     6721 cgccgaggca aaaatcaccc gcctgccgct gggctttttc ttcacactca cggtttcatt
     6781 tacgatcgcc gtcattccat gttccttgcc aaaaagtgtt gttgtgtgtt gttttattta
     6841 ttgcagccag gcttctacca gtgcgcccca gtagctgctt gccgggacca gacttgcatc
     6901 gttgaagtcg taacccgggt tatgcaccat gcaggaacct tccccgacat cgccattacc
     6961 gaataacagg aaaccgcctt tgggttgggc ttccagcatg aaagagaaat cttcactgcc
     7021 catcagaggc ttgatgccaa actcggcccg atcttcacca aacgttttac acgccacctg
     7081 ccaggcgaag cgcgccatct cctcgtcatt tactaatact ggtgtaccgt taacatgcgt
     7141 aacctcagcc gtagcgccaa agctggcagc ctgggcttgt gccagagcag gaatacgggt
     7201 cagaagaagt tgtcgggtat cgcggctaag agagcggacg ctgattttca tctcagcact
     7261 gtcaggaatg acgttagccg cctcacccgc aacaatgctg ccgacggtaa ttaccgccgc
     7321 ttccagcgga tcgacattgc gtgacacaat gctttgtaat gcggtggtga tatgtgcggc
     7381 aaccagcacc ggatcgatgg ctttgtgcgg gatcgcaccg tgtccgccac aaccacgaac
     7441 cgtaatatgg aactgatcca ttgacgccat cagtgcccct ggctggaaat aaaacttacc
     7501 caccggcaga ccgggcatgt tatgcatgcc gaaaatgaca tcgcagggga aatgatcaaa
     7561 aagcccctct ttaaccatga tctcaccgcc attaatcatc tcttccgcag gctggaagat
     7621 aagacgtaac gtgccgttaa aacggcgggt ttcggcaaaa taacgcgcgg cccccagcag
     7681 tatggtggta tgcccgtcat gcccgcaggc gtgcattaaa ccaggatgtt tactggccca
     7741 gagcttgcca ctattttcat aaattggcag cgcatccata tccgcccgca ggccaatact
     7801 tttctctccg tcgcccactt ttaacgtggc cactatcccc gtcgtcgcca gtccttgctc
     7861 tacttcatag ccccactgac gcagcttttc cgcaaccaaa gcactggttt taaattcttg
     7921 taatcccagc tccgggtttt cgtggatctg atggcggatc tcacgcagtt cttcctcaaa
     7981 ccctcgaata tagtgttcga tcatcggcta ttcctttatt cacttataaa caggctaatt
     8041 aacggatttt attgatggta taaatac
//
LOCUS       DAZGBC010000191         8008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGBC010000191 DAZGBC010000000
VERSION     DAZGBC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.192"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..709)
                     /gene="tli1"
                     /locus_tag="ACHK1J_004372"
     CDS             complement(32..709)
                     /gene="tli1"
                     /locus_tag="ACHK1J_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO7913559.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNEHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMNMCKGVTKHPDGYVYYGDTPDFIKGK
                     IYPYGNW"
     gene            complement(729..2411)
                     /gene="tle1"
                     /locus_tag="ACHK1J_004373"
     CDS             complement(729..2411)
                     /gene="tle1"
                     /locus_tag="ACHK1J_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFO7913560.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(2408..4927)
                     /locus_tag="ACHK1J_004374"
     CDS             complement(2408..4927)
                     /locus_tag="ACHK1J_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7913561.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLELALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(5006..5233)
                     /locus_tag="ACHK1J_004375"
     CDS             complement(5006..5233)
                     /locus_tag="ACHK1J_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913562.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            complement(5230..5871)
                     /locus_tag="ACHK1J_004376"
     CDS             complement(5230..5871)
                     /locus_tag="ACHK1J_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913563.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(5858..>8008)
                     /gene="tssH"
                     /locus_tag="ACHK1J_004377"
                     /gene_synonym="clpV"
     CDS             complement(5858..>8008)
                     /gene="tssH"
                     /locus_tag="ACHK1J_004377"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO7913564.1"
                     /translation="GADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPVSGRDT
                     EIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLRTLDLG
                     LLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKP
                     ALARGELRTIAATTWSEYKQYFERDAALERRFQMVRVDEPDDETACLMLRSLKSRYAE
                     HHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQLTRIRS
                     QLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENELMTARDDLEQQYARECELTGEL
                     LESRSDISRQSETHHLQQALHDIQQNQPLLSVDVDVRTVAGVVADRTGVPLSSLMKDE
                     QTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVGPSGVGKTE
                     TALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPY
                     SVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQL
                     SEKPETTESELHELIRPLLRDHFQPALLARFRTVIYRPLTQSAMRTIVEMKLAQVCER
                     LHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQH
                     PTYLSLTWNDEDGIVIAFDGINSDYPQESIYGQC"
BASE COUNT         2016 a         2206 c         1702 g         2084 t
ORIGIN      
        1 ttttatatgc tcatcattat tccggttgga gtcaccagtt gccgtagggg taaattttcc
       61 ctttgataaa atctggtgta tcaccgtaat aaacatatcc atctggatgt ttagtaaccc
      121 ccttacacat attcatttta tcacccgata ctgtagtaat tttagcatcc aactgaggta
      181 ccaccggatt accgcgatct cccagccaga ctcttacctt cccttcaggt gccagaccaa
      241 taatcatcgt tgaacgataa taggtttcgt tccagcggtt gtctgtgtaa ggcgtaacca
      301 tctgctccca ggttcctttt ttaaaaaaaa tcagagtttc ataactcttt ttatcaatta
      361 tcgaatccca gcaaaaacgc aacatcagag gtaatgcttt ccccttgtta taatacgact
      421 cagtcgcgcc cagatgctca ttccagcgcc ctacactgac ctgactcggt cttgtggtat
      481 caagatactg ataagtcgtc ttataacctt ccccgtccac catcaatgcc tgagtggcca
      541 gcgcaggcag agcctttgga taaataaaga gaaatttcca ttcatcatag ggtaattttt
      601 tataacccca ttcagtctta tccgaatcag catactgtga gctaacagaa ttacacccag
      661 ttaacaatgc caataatggt gcgataaaaa tgattttatc tctggtcatg tattgtcatc
      721 ctgtagcgtt aaacaccaga catcataact ttagtatttt ttactgcgtt atcattcaat
      781 cgctgctgat catccatgta ccatacagaa cgaatccagc gatcatttgg tttattcgga
      841 aaaacgaacg ccttattacc tttaggacca aagcgatgga taaaaatctc tcccgttgta
      901 ggatccaccc ataaattacc atcagactca atattccagt tggcagaaca atgggtgtat
      961 tttccgataa tatacaactc ttcctcagta aaagcctgag gctcttgccc caaacgtacc
     1021 gcttttccct gagcaatggc tttatcaaca aaaggaatta attcaggaga taaattcata
     1081 tctggatttt tggggtcaat tggagcaaac aatacccctg cttcctgagc agcatccagc
     1141 atcacacgca ggcaaacatt agcccagtca ttaggtactg ccatacgctc aaaaaatact
     1201 gcggctccca cctgcttttg tatcacgcca gcccgacgtt gattgctgtt gttcacacct
     1261 acggaacgaa ttttggtggt tagttttcca tgaggcaaaa tatgttttaa ggctggcaga
     1321 gtcatcaaca tttttctggc ttgctctgca ttacggtata ctcgcgtatc tgtttctctg
     1381 gtatcgtcac tgacaatttc aaatgccggg caactcagaa acagactttc attttcctgc
     1441 agaggtgaac ctaccggatt gtaaccacct ccaatatctg aatgcgcccc tggtaaagcc
     1501 agttcgggcc acataccttt aatactgttc aggctgaaat tatatcggta ttcattcatt
     1561 gcagtaatct ggaaaacttt cttcgcgatg gaaggacgca attccagctt tactcccggg
     1621 ttgctacgtc cattaatatc gaagaaatta ctgattccac caatagctgc gacagtatca
     1681 aaaagcccca gaaaacggac ttcacccgaa ggtttaccat cataaaaatc gcctctgagc
     1741 cctttagcaa tagctctggc tatagcagga tcctgttcca taacccgatt agcaaagtgc
     1801 cttgccgccg ctgctccccg gctaaaacca aaaacatcaa actggatttt tgcgatacaa
     1861 aaatcaggat ttaaattgaa ccccataaat tctgataacg cttgggtgat tcgttccatt
     1921 gcctcgtcgg ttttcgtcac cacaccttca aatatatcca gaatactggt gcctaatccc
     1981 atgccaatga ctgaatctgc ttcaccagcc gcagtcccta tcccggaaat gtaagtctga
     2041 atttgtgctt caacctgact ttccaacaaa gtctgatctg ctacgtaaag tgaatttagc
     2101 cagtggatat tggaataata tccacgataa ctgccattcg agatactgtt cttgttaatt
     2161 tcttcaagaa agtcctcaca gctttgtgca tctttttgat ttaccccctg tctggctgca
     2221 ctacactcat taaatttcat cagacgagat gctgtattct cccgattgtt tccggtacca
     2281 tcgaaaaata taccaatggt taaagtcaca ccccgcgccg gttttttctt tgcaacaaca
     2341 actttccagt cattatgaat actggtttta tgggtggcat ctgttacatc atatccctga
     2401 tattttgtca ttctgtttct ccatgaattt ttaccttccc aaactcaact tttactactg
     2461 acggaatgga ggtgttgact ggcgctgttt taccattttc atcggtgatc ccctgtagaa
     2521 tatcaccttc agccgttgtt aatcgataag gcgtcgaagg ttgcggcact cccgcttcat
     2581 cggtaagaat aaaaccgcca ccaaaccctc gcggcatttc aacagacgca atatcaagac
     2641 ttgttggtcc catcttctgc atattcgtgc tttttaacag gatctgtccc gggcatccca
     2701 gctcaatatt tccaccgctt agtttgatat aagctccacc acatgccagt atcagttcct
     2761 gattagcaga aacagtcact ttgccctcag aactttgaat atcaagattc tgttgggaac
     2821 tcattgacaa atcgtcactt tgagcctgaa tatttacccg cccttttgct gctaacagtt
     2881 gcatcccctg gtttctgctc aacaggctaa cagcaccttc tgctacaact gtaaatgact
     2941 gcccggcact aatatcagtg ttatctcccg acattatgcc cacgctttca ctacccgaag
     3001 caattcgtaa cgcccgtgca ctaaccatgc caatcccatc aggagcatgc aacagcattc
     3061 ccggcgcagt gagatctgtt agcgatgaat tcaaccgttg ctgacaactt atatctccct
     3121 gagtagcatt tgcagcagtt gcggccttcg ccatacttcg ggccagagat aatgccagct
     3181 ccagttggcg gatagcttca ttcatatcca gcatctttcc ttgtgcctga ggctgcatat
     3241 ctgcactgat aaatattcct tttcctcccc ggatacttat ccagtcatca gtcctcagct
     3301 cagcgccttc tcccctgagt ttccgggagg cattaacgtt atgaccaaga ttaagttgtg
     3361 tcttaccgcc aaactcggta ctgatcttga tatgctcctc accgcgtttg tcctccatcc
     3421 gcaacttgtt atttgccggc gtacggataa cattgcgcgt accgttctta tcggtaacgt
     3481 gatcaacatg acgtgaatca tgcagggcat gcgcgatata ggggcgatcc ggatcacctt
     3541 cctcaaaggc aatggccact tccgttccct gaatcagcgg aaaatgaaaa ccataagcat
     3601 caccactgta gggtttggcc agacgtaccg gcatgctttc ctgtcccgga tttttatcat
     3661 cacggtcagc atcaaatttc acccggtaca tacctgatgc atcctgccag gcgtagatat
     3721 ccccttcttt tgcactggtc acacgggccg tcatggtacc ggtaactttc ggacgcggca
     3781 gtaaaggagg acgccagcag ataacttcac tgtaaggaac gcccttcagt acaacctgca
     3841 gggcatcctt gcgacttgca gaaaaaccga cacaggtaag caacaccgga ttgcgcagca
     3901 gcgcaggcaa cgtcggcgga agactgtcgg tgatagtcag aacctgagca ggtgccagcg
     3961 tggcgtctgt actgttgccg gttatccgca cctgctccgc cagataacgt tcatgaccaa
     4021 ggcgggcata aaaattggcc gtctccggca ccgggtcgat tttatccccc cgttccagat
     4081 gacggaaatg gtagtggtac acctcaccat agttaattcc gtcaccatca ccatgcgtca
     4141 tgtctgccgg ggcggactgc aacagatatt gtgcttcacg atggttataa gcctttgtgg
     4201 tgacactggc ctctgccaca ttgtgcgtca cattcagccc ccacactgaa tccgctctgt
     4261 tatcattcat tccggacgga ctgtttatcg ccagcgtctt atcaaacgtc agcgcagcct
     4321 ggctgtcacc aaaatgcact acttccgtct gcgtgtccgg ttgcagggtg aaaaaataaa
     4381 aaatccccac ttcagccagc aaacgctgaa taaattgcag gtcactttcc tgatactggt
     4441 taatttgttc acgcttcgga tacgtttttt tgagacggaa ctcaaactcc cagcctttca
     4501 ggccgtgctc ctggagaacc tctgtcacca cctccggcac cgatttgtta acgaaaaatc
     4561 ggtgagaacg atgctgcctg tccagtagtt taataaatgg ttccagtatc acctggtatt
     4621 ttgcctggtc ggcggagccc gatatacgac gaaaatctgt aatgacacca tgtactactt
     4681 tctggctcac ctgctcctta agccctcccg tacccattgt aaatgtggct gatttacgca
     4741 gcagcagccc ggcatccagg tcgttgtcag tactggtaaa attgatgatg taataatagg
     4801 tctgactcat ctcctccctg ccgctgaaat gctcaacatc cagtggtgac gggcaggagg
     4861 gaataattaa atggtaacga ttcagggaag cagagatagt ttttgaaagt gtatctgtca
     4921 gactcatatt gttaactcca tgtctgtacc ggtattgaac tgcaattata taatgatgac
     4981 cgttaagctt tattttcaga gagaattact gataatcatc ccattcatcg taaatatatg
     5041 catatgcaaa tcgatacatc agataaccga caaaaaataa cgcaacagag agccctcccc
     5101 acagatattt aaattcagca gatgagagaa aaaagaacca gactgtaaat acaatacccg
     5161 acgcacctaa ccccatacct gcgccacgga taaatcggga aagcattata agtaatgttc
     5221 tttttatctt cataaaccac cgaaaaaata ttcgcatatc cggtcaaact cagccgaatt
     5281 tcggtctgcg acaatacagg ctcgttcaaa cgggcgcaca atatcttcaa caagaaagta
     5341 caaaagatcc aaatccccct gagctttcag cttatcatgc aacaccggat ttctgtcttt
     5401 cagaaaacgg gatgtgtaaa tcgcacgcga tgtctctgcg ccaacagata ataacaaccc
     5461 ggcaaaactc cctgcaaaaa aagcagccag ccctctggat accagaacct ctcccagttt
     5521 aaccccggtc aattcagcaa atgccattct acctgccaga tctcctgaaa cgttcttcac
     5581 aatggagcct gttttttcca tattgatccg ggatacaaag tcttcaagac aaatactgat
     5641 aattttccca ataatatcac ggctgaaaat gcctttttct accagtctgg ccattctgaa
     5701 tttgtcattc tggttttccc gcctatgccc ggtatctata aaatcatatc ccatataacc
     5761 gagactgact ggcaaagata tgagtccctt cccgacggcc cccagaaacc catccccatt
     5821 aatatttacg gcctccagca tatcccgggc aatttcttta gcactgtcca tatatgcttt
     5881 cctgcggata atcgctattg attccatcaa acgctatcac aataccatcc tcatcattcc
     5941 acgtcagaga caggtacgtc ggatgctgtc tggcagcaag atagctgagc aactgccggc
     6001 tcagtaccgg cagcagttgc tggttcagca gactctccac attccgggcg cccgtatccg
     6061 gcagcaggca ggcggatgtc agggcatcgt acacacgttc atcaaccgat gtgctcagcc
     6121 cataatggca gtgcaggcgt tcgcagacct gtgcaagctt catctccaca atggtgcgca
     6181 tggcagactg cgtcagcgga cggtaaatca cggtccggaa acgggcaagc agtgctggct
     6241 ggaagtggtc gcgcagcagt ggacggataa gctcatgcag ctccgattcc gttgtctccg
     6301 gcttttcgct aagttgctgc atcagaaggt cactgcccag attggaggtc atcagaatga
     6361 ccgtattacg gaagtcaatt tcacgcccct caccgtcgcg cataaagccc cggtcaaaca
     6421 cctggtagaa cagattcagc acatcccggt gggccttttc cacttcatcc agcagcacca
     6481 cgctgtaagg gcgtttacgc acagcctccg tcagaatacc gccctgaccg tatcccacgt
     6541 acccgggcgg tgacccctta agctgggaaa ccgtatgggg ctcctggtat tccgacagat
     6601 tgatggtaat cagtgatttt tcgccgccgt acatcacgtc tgccagcgcc agtgccgttt
     6661 cggtttttcc cacaccactg ggaccgacca gcaggaacac cccctggggg ccgttaccgg
     6721 atgtcagacc cgttttcgcc gcacgcagac gctgcgcaat ggattccagt gccacgtcct
     6781 gtccgaccac ccgtctgccg atatcctttt ccagatgcag cagttccgtc tgttcgtcct
     6841 tcatcaggga agataacggc accccggtcc ggtccgcgac cacaccggca acggtgcgta
     6901 cgtcaacatc cacactgagc aggggctgat tctgctgaat gtcgtgcagt gcctgctgca
     6961 ggtgatgtgt ctcactctgg cgggaaatat cgctgcggct ttcaagtaac tcaccggtga
     7021 gttcgcactc ccgggcgtac tgctgctcca ggtcatcacg tgctgtcatc agctcgttct
     7081 cttcctgctc aatggcagac agacgttcgc cgtgattctg atgaccaacg gcaatatctt
     7141 ccagcagtgc ctgtttttcc ataccgaggg aagcgagctg tgaacggata cgggtcagct
     7201 gttccggcac cgtgtcgagg ctcatacgca cacgggcagc agcagtatcg agcaggtcaa
     7261 cagccttgtc cggtaactgg cgttctgtca gataacggcg cgacagcgtg acggcagcac
     7321 gtaccgcctc atcggtgata tgcacgttat gatgttccgc ataacgggat ttcagggagc
     7381 gcagcatcag acaggccgtc tcatcatccg gctcgtccac tctgaccatc tgaaaacgcc
     7441 gctccagagc cgcgtcacgt tcaaagtatt gtttgtactc agaccaggtg gtggcggcaa
     7501 tggtacgcag ttcaccccgc gccagcgcag gcttcagcag gttggccgca tccgcgccgc
     7561 cggcctgatt accggccccg ataagggtat gggcttcatc tataaacagc agtacaggag
     7621 ccggtgactg ctgcacggca tcaatcacgt ttttcaggcg ctgttcaaat tcacccttca
     7681 cgccggcacc ggcctgcagc aggccgaggt caagggtgcg cagggtgacg ggtctgagtg
     7741 attccggtac gttaccttcc gcaatacgca gggcgagccc ttccaccaga gccgttttcc
     7801 ccacaccagg gtcccccacc agaatcgggt tgttcttgcg gcggcgggag agaatatcca
     7861 ccatctgacg gatttccgta tcgcgtcccg ataccgggtc aattttcccc tctctcgcgc
     7921 tggccgtgat gtcccgggtg aatttatcca gagcactctg taacacaggc ggcaggtcac
     7981 ctgacggtat acccgccgta tccgcacc
//
LOCUS       DAZGBC010000192         7945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGBC010000192 DAZGBC010000000
VERSION     DAZGBC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.193"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            255..2492
                     /gene="nrfB"
                     /locus_tag="ACHK1J_004378"
     CDS             255..2492
                     /gene="nrfB"
                     /locus_tag="ACHK1J_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO7913565.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2479..5451
                     /gene="nfrA"
                     /locus_tag="ACHK1J_004379"
     CDS             2479..5451
                     /gene="nfrA"
                     /locus_tag="ACHK1J_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO7913566.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5452..6342
                     /locus_tag="ACHK1J_004380"
     CDS             5452..6342
                     /locus_tag="ACHK1J_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO7913567.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQRSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6525..7286
                     /gene="envY"
                     /locus_tag="ACHK1J_004381"
     CDS             6525..7286
                     /gene="envY"
                     /locus_tag="ACHK1J_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO7913568.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7332..7408)
                     /locus_tag="ACHK1J_004382"
     tRNA            complement(7332..7408)
                     /locus_tag="ACHK1J_004382"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7372..7374),aa:Arg,seq:tct)
     gene            7651..>7945
                     /locus_tag="ACHK1J_004383"
     CDS             7651..>7945
                     /locus_tag="ACHK1J_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO7913569.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQA"
BASE COUNT         1960 a         2072 c         2039 g         1874 t
ORIGIN      
        1 ctgctcacct ccaggcactt ccactacatc atgacctgat cggtgccacc actcctcttc
       61 actatgacgt taaccccccc tccttgacac cacgcttttg tctacattct acgcaaacgg
      121 caacttttat aaccagtgta aaaataacgt gccgcaatag tcatacctat taatggtaaa
      181 aagctgtcac aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa
      241 tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa
      301 aagtaatcgc gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta
      361 ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc
      421 cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc
      481 cggcgtggaa tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg
      541 actacgaaaa ctatcatatc tttgttggca cctaccccaa cgaccccgat actcagcgtg
      601 atgttgacga agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg
      661 gccccaccag caaagccgac tgtctgaaca acgtgctgga cgccatcacc cagtttgagc
      721 gtagcgccaa tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac
      781 cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccgg
      841 tgtatccgtt tgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt
      901 cagagctgca tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg
      961 caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc tctgttagct gacggtgacg
     1021 gtattgcttt cgacgtgcag agtctgactg aagattacga cattggcttc cgcctgaaag
     1081 aaaaaggtat gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg
     1141 agcagcgtaa atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt
     1201 tccccgatac cttttcgact gcggttcgac aaaaatcccg ctggatcatc ggcattgttt
     1261 tccagggctt taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc
     1321 gcgaccgcaa aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc
     1381 agcttttgct gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta
     1441 ttttcagcgg cagcgcatgg ttaatgaccc tgctgtggct aaactttggt ttgatggtta
     1501 accgcatcgt gcagcgggtg attttcgtta ctggctacta cggcctgacg caggggctgc
     1561 tttccgtcct gcgtcttttc tggggcaacc tgattaactt catggccaac tggcgcgcgt
     1621 taaaacaggt acttcaacac ggcgatccac gtcgcgtggc gtgggataaa acaacgcatg
     1681 acttccccag cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg
     1741 aaaatcaggt catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc
     1801 tacgcctggg cggttcaatg ctgatgcagg ggctgattag cgccgagcaa ctggcacagg
     1861 cgctggcaga gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccttcct
     1921 cgctgattgc cgaaatgccg gcctccgtgg cgctgcatta tgcggtactg ccgctgcgtc
     1981 tggaaaatga tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg
     2041 ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg
     2101 tcacggggtt acgtcactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt
     2161 acaatgcggt tcagcatcag tggctcacgg aacagcaggc cggtgaaatc tggcggcaat
     2221 atgtgccgca tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt
     2281 cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt
     2341 tggtcaccga aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg
     2401 aattacaagt ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg
     2461 cgcaactgga gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt
     2521 ttactgctga cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca
     2581 gaagagctgg ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg
     2641 ctgaaggcac agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac
     2701 cagcaggtgc cggataatat tccgctgact ttataccttg cggaagccta tcgccatttt
     2761 ggtcatgatg accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat
     2821 gcccgacttg agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgacagtt
     2881 gaagaactgc ttgcccagca aaaagcgtgc gatgctgcgc cgaccctgcg ttgtcgcagt
     2941 gaagtcgggc agaatgccct gcggctggca cagttacctg tcgccagagc gcaactgaac
     3001 gatgcgacgt ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg
     3061 gcaatctacc tgaaacaatg gtcccaggca gatacgctat acaatgaagc acgccagcag
     3121 aacacattaa gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg
     3181 gacgatcgga tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt
     3241 acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt
     3301 gaaaataagc cgctgtttac cacggacgca caagagaaaa gttggctcta tctgttatct
     3361 aaatacagcg ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac
     3421 cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca
     3481 gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc
     3541 agcgtggcga cccgtaacaa ggctgaagct ctgcgtctgg cacgcttgct gtatcagcaa
     3601 gaaccggcaa atcttacccg cctggatcaa ctaacctggc aactgatgca gaacgagcag
     3661 tcacgggaag ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtatc
     3721 agccagactt taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctggcaacg
     3781 ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa
     3841 agtcagttgc cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg
     3901 tcgccttcct acgatgccgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta
     3961 cccggtgtgg cgttgtatgc atggcttcag gccgaacaac gacaaccgaa cgcctggcaa
     4021 catcgtgcgg tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc
     4081 tggcagaaaa tcagtcttca cgacatgagc aatgaggatc tgcttgctgc tgccaatacc
     4141 gcccaggcgg caggaaatgg tgcggctcgc gatcgctggc tacaacaggc agaacaacgt
     4201 ggactgggaa gcaatgccct ctactggtgg ctgcatgcgc aacgttacat tcctggtcag
     4261 ccggaactcg cactgaacga tctcacgcgc tcaatcaata ttgcgccttc tgccaacgct
     4321 tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg tcccggccgc ggtgagtgat
     4381 ttgcgcgccg cgctggaact ggaaccgaat aatagcaaca cccaggcagc gcttggttac
     4441 gccttgtggg atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa
     4501 gggcttccgg acgatccggc actgatccga caactggcct atgtgaacca gcgtctggat
     4561 gacatgcctg cgacgcagca ctacgcccgg ctggtgattg atgacattga taatcaggcg
     4621 ctgataaccc cattgacgcc agagcaaaat caacaacgct tcaatttccg ccgtttgcat
     4681 gaggaggtcg gtcgccgctg gacgttcagt ttcgattctt ctatcggctt gcgttccggc
     4741 gcaatgagta ccgctaacaa taatgtcggc ggcgcagcgc cagggaaaag ctatcgtagc
     4801 tacgggcaac tggaagccga ataccgcatc ggacgcaata tgctgctgga aggcgacctg
     4861 ctctcagttt atagccgcgt ctttgccgat accggagaaa acggggtgat gatgccggtg
     4921 aaaaatccga tgtccggcac cggtctacgc tggaagccgc tgcgcgatca gatctttttc
     4981 ctcgccgtcg aacagcagtt gccgctgaac gggcaaaatg gcgcatccga taccatgctg
     5041 cgcgccagcg cctcattctt taatggcggc aaatacagcg acgaatggca cccgaacggt
     5101 tccggctggt ttgcccaaaa cctgtacctc gatgcggcgc aatatatccg ccaggatatt
     5161 caggcgtgga cggcagatta tcgcgtcagc tggcatcaga aggtagctaa cggacagact
     5221 attgagcctt acgctcacgt tcaggacaac ggctatcgtg ataaaggcac tcagggcgcg
     5281 cagcttggcg gtgtcggggt ccgctggaat atttggaccg gcgagacgca ctacgacgcc
     5341 tggccgcaca aagtcagtct cggcgtcgag tatcaacata cctttaaggc gattaatcaa
     5401 cgtaacggag agcgcaacaa cgcgtttctc accattggag tgcactggta aatgcgtaag
     5461 ttcattttcg tattgctgac actgcttttg gtcagccctt tttcctttgc gatgaaaggt
     5521 attatctggc aaccacaaaa ccgagatagt caggttaccg atacccagtg gcaggggctg
     5581 atgagtcagt tacgtttgca aggtttcgat accctggttt tgcaatggac ccgttacggc
     5641 gatgcattta cccagccaga acagcgcacg ttattgttta agcgtgccgc agctgcgcaa
     5701 caggctggcc tgaagctgat tgtcgggctg aacgccgatc cggaattttt tatgcaccag
     5761 aaacagtcgt ccgcagcgct ggaaagctat cttaatcgcc tgctggctgc cgatctccag
     5821 caagccagat tatggagcgc cgcgcctggc ataacgccgg atggctggta catcagcgcg
     5881 gaaattgacg acctgaactg gcgcagcgaa gccgcccgtc agcctttgct aacatggtta
     5941 aacaacgcgc agcggctgat tagcgatgtt tcagcaaaac cggtttatat cagtagtttt
     6001 ttcgccggaa acatgtcgcc cgatggctat cgccaactgc tggaacacgt taaagcaacc
     6061 ggcgttaatg tctgggtaca ggatggcagc ggcgtggata aactgaccgc tgaacagcgt
     6121 gaacgttatc tacaggccag cgccgattgc caaagatccg ccccagcaag cggcattgtt
     6181 tatgaactgt ttgtcgccgg caaaggcaaa acctttacag cgaaaccgaa accggacgca
     6241 gaaattgcct cgctgttagc gaaacgttcc tcttgcggta aagacactct ctatttctct
     6301 ctgcgctatt tgcccgtcgc gcacggcatt ctcgagtatt aaatctcctc caggtaagtc
     6361 gggtacgacc tggcttacct ctttcgctct cgtcagaatt ccatcaagat taattgctaa
     6421 aaagcgtgct aaagaaagaa gatcgttatc ggcgaacggg ccgatcactc atgcaggaag
     6481 catagatcct ttgagcggag tttctgatat ctgaggagtg cgaaatgcaa ttgagcagca
     6541 gtgaaccttg cgtggtgatc ctgaccgaaa aagaggtaga ggtaagcgtc aataaccatg
     6601 ctacgtttac ccttccgaaa aactacctgg ccgccttcgc gtgcaacaat aacgtcattg
     6661 aactctcaac gttaaatcac gtattaatca cccacatcaa ccgtaacatc atcaacgatt
     6721 atctgttgtt tttaaataag aacttaacct gtgtaaagcc ctggtcgcgg ctggctaccc
     6781 cggttatcgc ttgtcatagc cgtacaccgg aagtgttccg gctagccgcc aaccacagca
     6841 agcagcaacc cagcaaaccc tgcgaagcgg agttgacgcg cgcattgctt tttaccgtat
     6901 tgtctaactt tcttgagcaa tcgcggttta ttgccctact gatgtatatc ttacgcagca
     6961 gcgtccgcga cagcgtctgc cgcattattc aaagcgatat tcagcattac tggaatctgc
     7021 gaattgtcgc cagttcgcta tgtttaagcc ccagcctgct caaaaagaaa ttaaaaaacg
     7081 aaaataccag ctatagccag attgtcacag agtgtcgtat gcgttacgcc gtacagatgt
     7141 tattgatgga taacaaaaat atcactcagg tggcgcaatt atgtggctat agcagcacgt
     7201 cgtactttat ctctgttttt aaggcgtttt acggcctgac accgttgaat tatctcgcca
     7261 aacagcggca aaaagtgatg tggtgaaggg caaaccggaa acggataaga cgggcataaa
     7321 tgaagaagaa atggcgcgcc ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg
     7381 ttgctctatc caactgagct aagggcgcgt tgataccgca atgcggtgta atcgcgtgaa
     7441 ttatacggtc aacccttgct gagtcaatgg cttttgatct ggttgctgaa caagtgaacg
     7501 accgcgtctg attttctgat ttatttcgct atagcggcaa acaaatgcac accgctgcgc
     7561 gtctgaatca agaaaacccg tattttcatg tatcaaagta caatttaccg ccctaacgga
     7621 aaattgtccg ctcctatgag actggtaact atgaaaccaa cgtcggtgat cattatggat
     7681 actcatccta tcatcagaat gtctattgaa gttctgttgc aaaaaaacag tgaattgcag
     7741 attgtcctga aaacggatga ttaccgcata accatcgatt atctccgaac ccgtcctgtt
     7801 gatttaatca ttatggatat agacttgccc ggaacagacg gttttacctt cctgaaaagg
     7861 atcaaacaaa tccagagcac agtgaaagtg ttatttttat catcgaaatc agaatgcttt
     7921 tatgctggca gagcgataca agctg
//
LOCUS       DAZGBC010000193         7916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGBC010000193 DAZGBC010000000
VERSION     DAZGBC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.194"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            362..811
                     /gene="alaE"
                     /locus_tag="ACHK1J_004384"
     CDS             362..811
                     /gene="alaE"
                     /locus_tag="ACHK1J_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO7913570.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKISPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(848..1192)
                     /locus_tag="ACHK1J_004385"
     CDS             complement(848..1192)
                     /locus_tag="ACHK1J_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO7913571.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1344..1673
                     /locus_tag="ACHK1J_004386"
     CDS             1344..1673
                     /locus_tag="ACHK1J_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO7913572.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1823..3157)
                     /locus_tag="ACHK1J_004387"
     CDS             complement(1823..3157)
                     /locus_tag="ACHK1J_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7913573.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGENQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3245..3676
                     /locus_tag="ACHK1J_004388"
     CDS             3245..3676
                     /locus_tag="ACHK1J_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO7913574.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIAKT
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3885..4130
                     /gene="nrdH"
                     /locus_tag="ACHK1J_004389"
     CDS             3885..4130
                     /gene="nrdH"
                     /locus_tag="ACHK1J_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO7913575.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4127..4537
                     /gene="nrdI"
                     /locus_tag="ACHK1J_004390"
     CDS             4127..4537
                     /gene="nrdI"
                     /locus_tag="ACHK1J_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO7913576.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4510..6654
                     /gene="nrdE"
                     /locus_tag="ACHK1J_004391"
     CDS             4510..6654
                     /gene="nrdE"
                     /locus_tag="ACHK1J_004391"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7913577.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFDHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VVDERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYNENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6664..7623
                     /gene="nrdF"
                     /locus_tag="ACHK1J_004392"
     CDS             6664..7623
                     /gene="nrdF"
                     /locus_tag="ACHK1J_004392"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7913578.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKRLEKISEAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         1997 a         2017 c         1934 g         1968 t
ORIGIN      
        1 ctgttaatgt acattttctg gcataaaaaa tgctgtaaat gacccggtca cgacatacac
       61 cgaattctct acgcttatag cgttacctca cccccaaact ttcattagtg aaattaaagg
      121 aaaggtcata tctaattgat ttaattgaaa ataaatcata tcggcatgta aaaatccaca
      181 atgtacaaaa aacgagccgt tacggaatat tttatctaca aaaactgact aaataaaaat
      241 ttttcactaa ttgattagtc atagtcagcg atatacgcta tgcgaaaatg cagatggcaa
      301 tgagatccac tgctttcatc tccattaaca tcccattacg cttttattaa ggagcattag
      361 catgttctca ccgcagtcac gcttgcgtca tgcagttgca gatacgttcg cgatggttgt
      421 ttactgttct gtcgtgaaca tgtgtattga agttttcctc tccggaatga gcttcgaaca
      481 gtctttttat tccagattgg tagcgattcc ggtgaacatc ttaattgcat ggccatacgg
      541 tatgtaccgc gatctgttta tgcgcgcggc acgcaaaatt agcccgtcgg gctggataaa
      601 aaatctggcg gatatcctgg cttatgtgac gttccagtca ccggtgtatg tggcgatctt
      661 gttagtggtg ggcgcagact ggcatcagat tatggcggcg gtcagttcaa acatcgttgt
      721 ttcgatgttg atgggggcgg tttatggcta cttcctcgat tattgccgcc gactgtttaa
      781 agtcagccat taccagcagg taaaagcctg actcttcttt tcgcgactgg catcgccagt
      841 cgcgtcttta actggcttcg ccaaataaac cattcaaata acgttcaagc gcaacacgcg
      901 aactaaagcc atgcgggata ccgtaatgct cgtgtcgctc gcctgctaaa tagagcggaa
      961 attgttgata atgatcgcaa gttttaatat cggaagcggg ttcagccagt gtcgaactac
     1021 gttcttttaa tgttggatga attaccagtg cggtacgtcc cattcgcgct tcgcgattaa
     1081 catagacata attttcccca cggcggtaac catacgcttt ctgggttacc acatcgacag
     1141 taaaaccgac tttttcaagt acgcgcgcca cctcgtctgg tcgtaaatac atatttgatc
     1201 ctcgttatta tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
     1261 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
     1321 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
     1381 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
     1441 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcat
     1501 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcacgcg
     1561 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagt gtgggaactg
     1621 ctgctgcggt aggtattttt atcggtgcgt tgttgagcat gcgtaaatcg tagtgagacg
     1681 atgacaagca gtagtctgac ttctgaaaag tactcagtgc gtcaacgcct tgtccgccta
     1741 caggcctgaa aaacatagcg catcagggga ttttgccctg gtcaccagtc ttataggacc
     1801 ccgaagcctg gcttcggggt tattaattta aagcactgcg caattgctgt gctacctgtc
     1861 gcgccatact ttcgctaacg atattcgtaa acgagagcaa taatcctccg tcctcgcttg
     1921 agcggatacg ccagtcgcta agtgcctgta cggcaagacc agcagcattg gctttatgca
     1981 cgaatgtcac gtcattacct gctagtttga tcaccatctg gataccgcct ttttgcggca
     2041 caacctgaaa tccctgctgc gtaagtgcct gctcaatcca ctgccgtcgc tgggcataat
     2101 gctggcgcat tttcttcaga tgccgccaga aatgcccctc acggatgaaa tctgccagtg
     2161 tgttctgcca cagtacgggt acagcacatg gttccagtga cgcctgctgc cggaattgcg
     2221 aaatctgctc caccggcacc accagccacg cacagcgcaa tgcaggaaat agcgctttgc
     2281 tgaatgttcc ggcataaatc acccgctgcg gcgcgtcgag actttttagt gccggtaacg
     2341 gcttaccgtg atagcgaaac tcactgtcgt aatcatcttc aataatccat gcctgactac
     2401 gctctgccca ttccagtatc tgatacctac gcgctaaaga gagtgccaca cccagcgggc
     2461 tttggtgtgc cggagtaatc agggcaaaac gcgcatcagg ataattttgt attccgcttg
     2521 tgacatccag tccgttgtca tcaaccggca caggtaaaat ttccacaccg tggcgagtga
     2581 caatcgggcg aatcagcgga aagccgggat cttccatcca catcccgtct cccggttgcg
     2641 ccagagcgtg cagaattaaa gccattgagg ccgcataacc gtgagtgata aagacctgct
     2701 ctggctgaca atcaatccca cgtgaaacgc gtaaataatc aacaatagcc tcgcgcaagg
     2761 ccgcctcgcc gcagacatcg cctaatgcca aatcaaaacg cgtctgggta cgcagacgac
     2821 ggcccatcac ccgcgcccac aattcacgcg gaaaaagatc aagtgcaggt agccccatct
     2881 gaaaaggcag cggctgctga ttttcaccag caaaagccac gacatccaca gcagactgtt
     2941 gcggatgtat ctgctcactg acaaaggtac ccgcctgtcc ccgcctgacc agccatccct
     3001 gcgccaccag ctcagcataa gcattttcga cagtggatcg agagacaccc agctcctgcg
     3061 accaggtacg gctcgagggt agccttgcgc caggtttcag ttctccttgc tcgatagccg
     3121 tttttaactg acgggcgata tcctgataac gtggcatatg gtctgctttt ttaggtaatt
     3181 tatggctctc tttagcagtc cattatagtg ctacattgcg ggcgttaata agaggagaaa
     3241 ctgtatgact acattacgtc agccttacta cgaacttagc ccggcagtgt ataacgcact
     3301 ggtgcaggcc aaaacggcac tggaaaatag cacgctggat accacgttga tggagttaat
     3361 ttatttgcgc gtctcgcaaa tcaacggctg cgcattttgt ctggagatgc acagcaaagc
     3421 attgcgcaaa tccggtgtgc cacaacacaa actggacgcc ctggcaggct ggcgcgtaag
     3481 ccatcatttt gatgaacgcg agcgagcggc gctggcatgg gcggaatcag tgaccgatat
     3541 tgccaaaacc catgcggaag acgaggttta tcagcctttg cttgagcatt tcagcgcagc
     3601 agaaatcagc gacttaacgt ttgccatcgg gctgatgaat tgttttaacc gtctggccgt
     3661 ttccatgcgg atgtaatttg ctgttgccgg gtatgcgccc ggcaaaaaaa cacaaaatcc
     3721 cctaccccgt tgagccattc accggtggcg taatcaatct aaaattccca tatcttgtgt
     3781 ggttgaatct tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg
     3841 cgtatcatcg cgccgtatct tcattttaaa cggaaatacg aatcatgcgc attactattt
     3901 acactcgtaa cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccggggct
     3961 ttgattttga aatgattaat gtcgatcgcg ttcctgaagc ggcagaagcg ttgcgtgctc
     4021 agggctttcg tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc
     4081 cggacatgat taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt
     4141 ctacttctcc agcagctccg aaaacacgca gcgttttatc gaacgtttag gtctgcccgc
     4201 ggtgcgcatc ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat
     4261 cgtgccctct tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt
     4321 tttaaacgac gagcacaacc gggcgttgct tcgcggcgtt attgcttcgg gtaatcgcaa
     4381 ctttggtgag gcgtatggcc gcgctggaga tgtgattgcc cggaaatgcg gcgtgccgtg
     4441 gctgtaccgc tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt
     4501 aaccgaattt tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac
     4561 gcgctgaatg cgatgcttaa cctctacgat agcgcaggtc gcattcagtt cgataaagac
     4621 cgccaggccg ttgacgcctt tattgcgacg catgtgcgtc cgaacagtgt gaccttcagt
     4681 agccagcagc agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt
     4741 aatcgctact ctcgcgactt tgtcattacg ctgtttgacc acgcacacac cagcggtttt
     4801 cgtttccaga cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc
     4861 gacggtaaac gttatctgga agattttgcc gatcgagtca cgatggtggc gctgacgctg
     4921 gcacaaggcg atgagacgct ggcgttgcaa ctgacagatg aaatgctgtc aggacgcttt
     4981 cagccagcca cgccaacatt cctcaactgc ggtaagcagc agcgcggcga actggtttcc
     5041 tgttttttgc tgcgtattga agacaatatg gagtcgattg gtcgggcggt aaattccgca
     5101 ctgcaactgt cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct gcgggaagcg
     5161 ggcgcgccaa ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg
     5221 ctggaagacg cattttccta tgccaaccaa ctcggcgcgc gtcagggggc tggggcagtc
     5281 tatttacatg ctcatcatcc cgatattctg cgttttctcg acacgaaacg ggaaaatgcc
     5341 gacgaaaaaa tccgcattaa aacactgtcg cttggcgtgg tgatcccgga tatcactttc
     5401 catctggcaa aagagaatgc gcagatggcg ctgttttcgc cttatgacgt agagcgagtt
     5461 tatggcaagc cgtttgccga tgtggccatc agtcaacact atgacgaact ggttgtcgat
     5521 gaacgcattc gaaaaaaata cctcaacgcc cgtgatttct tccagcgact ggcagaaatc
     5581 cagtttgagt ccggctatcc gtacatcatg tatgaagaca cggtaaaccg tgctaaccct
     5641 atcgccgggc gcataaatat gagtaatctc tgctcagaga ttttgcaggt taacagcgcc
     5701 tcagagtata acgagaatct cgactatgcc cgcacaggcc atgatatctc ctgcaattta
     5761 ggttcgttga atattgccca caccatggat tcccccgact ttgcccgcac ggtagagact
     5821 gccgtgcgcg gtttaacggc agtatcagat atgagtcata tccgcagcgt gccgtccatc
     5881 gaagccggaa atgccgcctc gcacgccatc ggactggggc agatgaattt acacggctat
     5941 ctggcgcgag aaggcatcgc ttatggttcg ccggaagcac tggatttcac caatctctat
     6001 ttctatgcca tcacctggca cgcactgcgt acctcgatgt tgctggcacg cgaacgcggt
     6061 gaaaccttcg ccgggttcaa acagtcacgc tatgccagcg gtgaatattt tagccaatat
     6121 ctgcaaggta actggcagcc gaaaaccgcg aaagttggcg aactgtttgc ccgcagcggt
     6181 attacgttac ctacccgtga gatgtgggcg cagctgcgcg atgacgtgat gcgctacggc
     6241 atttacaacc agaatctcca ggcggtgccg ccaaccggtt ctatctctta tatcaaccat
     6301 gctacgtcga gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca
     6361 ggacgcgttt actaccctgc cccgtttatg actaacgaga atctggcgct gtatcaggac
     6421 gcttacgaaa ttggcgcaga aaagatcatc gacacctacg cggaagcgac tcgccatgtc
     6481 gatcaggggc tgtcgctgac gctgtttttc cccgataccg ccaccactcg cgatatcaac
     6541 aaagcgcaga tttacgcctg gcgcaaaggt atcaaaacgc tctattacat ccgcctgcgt
     6601 cagatggcgc tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata
     6661 tctatgaaac tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat
     6721 ctggaggtgt ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccactgtcg
     6781 aacgatatac ctgcatggca gacattaagt gtcgtagaac aacaactgac aatgcgcgtt
     6841 tttactggcc tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttcgctgatg
     6901 cccgatgcgc tcacgcccca tgaagaggcg gtattatcga atatcagctt tatggaagcg
     6961 gttcatgccc gttcttacag ttcgattttc tcgacgttgt gccagaccaa agatgtcgat
     7021 gccgcctacg cctggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag
     7081 caacattatc gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct
     7141 tttttgttct attccggttt ctggctgccg atgtattttt ccagccgcgg aaagctgacc
     7201 aataccgcgg acctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata
     7261 ggctataaat atcagaaaag actggaaaag atctccgagg cacaacgtga agagttgaag
     7321 agtttcgcct tcgatttgtt gctagaactc tatgacaacg agttgcaata taccgatgag
     7381 ctgtacgccg aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacgccaat
     7441 aaggcgttga tgaatctggg ctacgaacct ttatttcccg cagaaatggc ggaagtgaat
     7501 ccggcaatcc tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt cttttccggt
     7561 tcaggctcct cttatgtgat ggggaaagcg gttgaaacag aagatgaaga ctggaatttc
     7621 tgagggtgtt attttcaaaa atatcactac ccgcgacagg gaaataattc ccgccaaata
     7681 gctttttatc acgcaaatat tttgtggtga tctacactga tactctgttg cattattcgc
     7741 ctgaaaccac aatattcagg cgttttttcg ctatctttga caaaaaatat caacttttct
     7801 cgatttgctc tcagccctta tatcacggga aattccggcg atttgctcac atcaatattc
     7861 atgccacatt tgccctcagg ggttgcctca gattctcagt atgttagggt agaaaa
//
LOCUS       DAZGBC010000194         7887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGBC010000194 DAZGBC010000000
VERSION     DAZGBC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.195"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(351..1544)
                     /gene="tyrB"
                     /locus_tag="ACHK1J_004393"
     CDS             complement(351..1544)
                     /gene="tyrB"
                     /locus_tag="ACHK1J_004393"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO7913579.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNALPHGASLYLPMEGLNSYRHAIAPLLFGADHSVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1797..2876)
                     /gene="alr"
                     /locus_tag="ACHK1J_004394"
     CDS             complement(1797..2876)
                     /gene="alr"
                     /locus_tag="ACHK1J_004394"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO7913580.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2929..4344)
                     /gene="dnaB"
                     /locus_tag="ACHK1J_004395"
     CDS             complement(2929..4344)
                     /gene="dnaB"
                     /locus_tag="ACHK1J_004395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO7913581.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4427..5410
                     /locus_tag="ACHK1J_004396"
     CDS             4427..5410
                     /locus_tag="ACHK1J_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO7913582.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5576..5818)
                     /gene="pspG"
                     /locus_tag="ACHK1J_004397"
     CDS             complement(5576..5818)
                     /gene="pspG"
                     /locus_tag="ACHK1J_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO7913583.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5952..6989)
                     /gene="dusA"
                     /locus_tag="ACHK1J_004398"
     CDS             complement(5952..6989)
                     /gene="dusA"
                     /locus_tag="ACHK1J_004398"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO7913584.1"
                     /translation="MYGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7352..>7887)
                     /locus_tag="ACHK1J_004399"
     CDS             complement(7352..>7887)
                     /locus_tag="ACHK1J_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO7913585.1"
                     /translation="GLPSTVQLFYEESKDNEPLANIHLQAIGSGFLTFVNACQKYDDN
                     SLKLFASLLISLSYSSAYTDLAGAEKVNINDDNENYLTAQFEELSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGKLAPDILSKMRDAAPKLIDFAKSFDPNSKEKIKIY
                     TGTSKLIYDLFGVKSEK"
BASE COUNT         2056 a         2044 c         1871 g         1916 t
ORIGIN      
        1 agagttctct ttaactttag aatttaacga tactataatt gaatctcaga aagtcacagg
       61 caataaggca aagcatattc ttgaggatat agaaaaattc tatcgtaaca aacccgatac
      121 ttattattaa atttaataat ggttagatgt gcagaaatta ttactccaca caaaaaaaca
      181 ggataaggaa ttccagataa ttgggataag cttaacaata aaagaactaa caacgtttat
      241 tatctcttac gtagaacgat ggcatcaaaa aatcatctat aaagaaagag tgaaagcgac
      301 gacagtttaa tttcactgca ggctgggtaa ctccagcctg ctttcctgca ttacatcacc
      361 gcagcaaacg cctttgccac acgttgtaca tttgccgtat ttaacccggc gacacacatg
      421 cgaccgctgg cgatgagata gacaccaaat tcttcacgta gtcggtcaac ctgagcggca
      481 cttaaaccgg tataactgaa catgccgcgc tgattaagca gataatcgaa attgcgttct
      541 ggcatctctg tgctcaatac cttcaccagt tcctgacgca ttgccagaat gcgagtacgc
      601 atctcttcta cttccgccag ccagctggct ttcaatgcct cgtcattcag caccgcagcc
      661 accacctgcg caccaaaatt cggcgggctg gagtagttgc ggcgaactgt tgctttcaat
      721 tgccccagta cgcggcctgc agcttcggca tcttcacaca gaacagaaag tccgccgacg
      781 cgctcgccgt aaagggagaa aattttcgag aacgaattgc tcaccagagc gggtaatcca
      841 gcgctggcaa tggcgcgaat ggcgtaggca tcctcttcca taccggcacc aaatccttga
      901 taggcaatat caaggaatgg gataagctcg cgggctttga gaatttcaat caccgcatcc
      961 cactggtcat tagtgagatc ggcacccgtt gggttgtggc aacatggatg cagcaacaca
     1021 atactgcggg caggtaatgt tttcagcatc gccaacaggt cattaaagcg cacgccgtta
     1081 gtcgcttcgt cataccaggg gtaagtactt acttcgaatc cggccccggc gaatattgct
     1141 acgtggtttt cccaggtagg atcgctgacc cagacgcctg attccggaaa gtagcgtttc
     1201 aggaaatccg cgcccacttt caatgcccct gagccgccaa gggtttgaat ggttgctacg
     1261 cgctgttgtt gcagtaccga atggtcggca ccaaacagca gcggcgcaat ggcatggcga
     1321 tagctgttaa gcccttccat cggtaaataa agcgaagcgc catgaggcag cgcattcagg
     1381 cgcgcttctg catcagccac ggctttcaat tgtggaataa ttccgtcttc gttgtagtac
     1441 agaccgatac ttaaattcac tttgtcgctg cgagggtctt ctttaaaacg ctccataagc
     1501 gtaagaatcg ggtcgccagc gtaggcgtca actttttgaa acacgcgatg gttctccagg
     1561 tttacgggca ggtggttaaa acacaataaa ccggaagaag gcgaagatcg agtggatgtt
     1621 caggggcgac cggcaattag caacagagtg agactcatga caaacgtaca tccgccagag
     1681 gcacgacctt tataagagca tgaaagaata tcaacttatt gaattggtag gattttagtg
     1741 gccggataag gcattcacgc cgcatccggc acagacaatc aaatattaca gaacgattaa
     1801 tccacgtatt tcatcgcgac ccttgaagtc aggcgcgtaa taagttcgta agcgcttact
     1861 ttcgtcattt cagcgatacg ttctacgggc aaaccttcac cccacaaaat gaccgggtcc
     1921 ccggctttat cctgtgcctg tggacctaag tctacgcaga tcatatccat cgcgactcgc
     1981 ccgacaatcg gcacttcgcg accgttcacc agcactggcg taccagacgg cgcggcgcgc
     2041 ggataaccat cgccatagcc catcgcaact acaccaagac gagtatcacg ttcgcttatc
     2101 caggttccac cataaccgac aggctctccg gctttatgct cacgcacggc aatcaggctg
     2161 gaggttagtg acatcactgg ctgacagcca aaatcggcac cggtggagcg atcttccagc
     2221 ggcgagacgc cataaagaat gatgcccggg cgcacccagt caaaatgcga ctgtggccac
     2281 agcagaatgc cacccgacgc ggcaatggaa cgttgacctg gtttgccttc gcaaaaggta
     2341 ttaaagatag cgagttgttt ctcggttgcg ccacattttg gttcatccgc gcgcgcaaaa
     2401 tggctgacga tattcaccgg ctgacgaacg tttttgcact gggtcaggcg atgataaaac
     2461 gcctcagcct gttccggcct tacgcccaga cggtgcatac cggtatcgag tttcatccag
     2521 acggtaaccg gctcgtccag gctagcctct tccagcgcag ccagctgttc ttcgttatgc
     2581 acggcggtat gaaaatgttg cgcagaaatc gtcggtagat ctctggcatc aaaaaagcct
     2641 tcgagtaaca gtacaggttt ggtgattccc cccgcacgca gtcgcagagc ttcttcgaga
     2701 cgggctacgc caaaggcgtc agcatcgggg agcgttcgcg cggtctcaag aagaccgtga
     2761 ccataagcgt tcgctttcac caccgcaacc attttactgg caggagccag ttcacgaaga
     2821 cgttgcaggt tgtgtcgcag agcgcggcgg ttaatcacaa cagttgccgc ttgcatttgt
     2881 gttccttgat aagtgtttgc tttaattacc taattcataa aataattatt attcgtcgtc
     2941 gtactgcggc cccgcatagt tgtcgaagcg cgaccattga ccgttaaagg tcaggcgtac
     3001 cgtcccgatt gggccgttac gttgtttacc gataataatt tccgcgatgc cttttaaatc
     3061 actgttttcg tgatacacct catcacgata gataaacatg atcaagtccg catcctgctc
     3121 gatagagcca gattcacgca ggtcggagtt gaccgggcgt ttgtcggcac gttgttccag
     3181 agaacggttc aactgggaca gcgccaccac cggcacgttc agttctttcg ccagtgcttt
     3241 cagcgagcgg gagatttctg caatttccag cgtacggtta tcggaaagcg ccggtacgcg
     3301 catcagttgc aggtagtcga tcatgataag cccgatgccg ccgtgttcac gggcaatacg
     3361 gcgtgcgcgg gaacgcactt ccgttggcgt caagccggag gagtcatcga tatagatatt
     3421 gcgtttttcg agcaaaatac ccatggtgcc ggaaatgcgt gcccaatctt catcatcgag
     3481 ctgcccggta cggattttag tctggtcaac gcgcgacagc gacgccagag aacgcatcat
     3541 aatttgttct gatggcatct ccagcgagaa gataagtacc ggtttatcct gcaacatcgc
     3601 cgcgttttcg acgaggttca tcgcaaatgt cgttttaccc atcgacggac gcgcggcgac
     3661 gatgatcaaa tccgacggct gcaagccagc ggtttttttg ttgagatcgt cataaccggt
     3721 gtttaccccg gtaacgccat cgtgtggctg ctgaaacaac tgctcaatac gcgccacggt
     3781 ggcgtcgagc acatcggcga tgttcttcgg cccttcgtct ttgttcgcac gactttcggc
     3841 aattttaaag acgcgggatt cagccaggtc cagcagatct tcgctggtac gcccctgcgg
     3901 atcaaaaccg gcttcggcaa tctcattcgc aaccgagatc atctcacgga caacggcacg
     3961 ttcacgcacg atgtccgcat aggcactgat gttcgccgca cttggcgtat tttttgacag
     4021 ctctgccaga taagcaaaac caccgacgct atcgagttgc ccctggcgtt ccagcgattc
     4081 cgcaagggta atcagatcga taggactacc gctttcctgc aaacgcgcca tttcagtaaa
     4141 gatatgacgg tgtgggcggg tgtaaaaatc gtctgctacc acacgctcgg ctacatcatc
     4201 ccagcgttcg ttatccagca ttaaaccgcc caacaccgac tgctccgctt cgatcgagtg
     4261 cggaggcact ttcagcccgg caacttgtgg atcgcgttcg cggggttcag cctgctgttt
     4321 gttgaagggt ttatttcctg ccatagtgaa tggagttacc gagataaaga atgggtcgaa
     4381 actttaccat atgaagcaga ccctgacgat acgttctgga ggacacatgg caacacgaat
     4441 tgaatttcac aagcacggtg gcccggaagt acttcaagcc gtagagttca ctcctgccga
     4501 tccggcggag aatgaaatcc aggtcgaaaa taaagccatc ggcatcaatt ttatcgacac
     4561 atatatccgc agcggccttt acccgccgcc atcgctaccc agcggattag gcaccgaagc
     4621 agcaggcata gtgagtaaag tcggcagtgg tgtaaagcat attaaggcag gcgatcgtgt
     4681 agtctatgcg cagtcggcgt taggcgctta cagctctgtg cataacatta ttgcggataa
     4741 agcggcgatt ctgcctgcgg caatttcttt tgagcaagct gcggcatcct tcctgaaagg
     4801 cttaacggtt tattatctgc tgcgcaaaac ctatgaaatt aaacccgatg aacagttcct
     4861 gttccacgca gcggctggcg gcgttggctt gattgcctgc cagtgggcaa aagccctggg
     4921 cgcgaaactt atcggcaccg taggaaccgc gcaaaaagcg cagagcgcgc taaaagcggg
     4981 cgcgtggcag gttattaact atcgtgaaga gaatctggtc gagcggttaa aagagatcac
     5041 cggtggtaaa aaagtgcgcg tggtgtacga ttccgtgggc agagacacct gggaacggtc
     5101 gctggattgc ctgcaacgcc gcggcttaat ggtgagtttt ggcaactcat caggtgcggt
     5161 taccggtgtg aacttaggca ttctcaatca gaaaggctcg ttgtatgtga cacgcccttc
     5221 cctgcaaggc tatatcacca cgcgggagga attaaccgag gccagtaatg aactgttctc
     5281 tttgattgcc agcggtgtga ttaaggtcga tgtcgccgag cagcagaaat atccgctgaa
     5341 ggatgcgcag cgtgcgcatg agattctgga aagccgggcg acgcaaggtt ccagcctgtt
     5401 gattccataa aagaaatagg gcttccacct gggaagccct ttctttttat agttcggctg
     5461 tatgtagggt acagcacgat gaatctgtta gaggcgcaat agtgacagat ttgattatca
     5521 attcctattt tgttctaagg ataaaacctt aggttgtgat catccgcaca atcccttagt
     5581 aacgccagcg gtcataacgc tgatatttcg gcacttttgg tgctttaatc gccttaataa
     5641 cccacaccac cgcaatcgcc aggagtaacc acggcagcaa cttaatcatc aatgccagca
     5701 taccgccgag gaacataatg gccgtcgcca caaccagcgc ggcgataatg cccagcaacg
     5761 aaacgccggt gaccatcaac atgacaaaaa agccaatcac aaaaagtagt tccagcatga
     5821 tgctctccca aatatgaaat ctcttgctgg cattacaaga atcatgccaa aaataatcta
     5881 ttgatttaac agcaaaacgc cccgcgaatt tgcgcagggc gtggtgaatt tgactacttt
     5941 ttggtgaaaa gttaacgctt atccgccacc agtttgagcg cgtgttccag cacgttaatg
     6001 tcagcacccg ctttatgggc attttcactt aaataacgcc gccactgccg cgcgccagga
     6061 ataccctgga acaagcccag catatgccgg gtaatatggc cgagatacgt cccctggctg
     6121 agttcacgct caatgtacgg atacatggcg cgcactaccg ccaccggatc ggcatcggta
     6181 tccgaggaac caaagatctc tcggtctacc gccgccagaa tacccggatt ctgatacgcc
     6241 tcgcgcccga ccatcacgcc atccatatgt tgcaaatgcg ctttagcctc ttccagcgac
     6301 ttgataccac cgttaatcga catcgtcaga tgcggaaagt cacgcttcag ttgatacaca
     6361 cgcggataat cgagcggcgg gatttcacgg ttttctttcg ggcttaaccc cgaaagccag
     6421 gctttacgtg cgtggatgat gaacatctca cactcgcctt tgccggaaac ggtgttgatg
     6481 aaatcgcaga gaaattcata gctgtcctgg tcatcgatgc caatacgcgt tttcaccgtc
     6541 accggaatcg acaccacatc gcgcatcgct ttcacgcagt cggcaaccag ctgcgcatta
     6601 cccatcagac acgcaccaaa catgccgttc tgcacccggt cagacgggca gccgacattc
     6661 aggttgatct catcatatcc acgcgcttct gccagcttcg cacactgcgc cagcgccgcc
     6721 ggatcgctac cgccgagttg caacgctacc ggatgttctt cttcactgta cgccaggtaa
     6781 tcacctttac cgtgaataat cgcccctgta gtcaccattt cggtatacag caacgtattg
     6841 cgggaaagca gacgcaagaa atagcggcaa tgtctgtccg tccagtcgag cattggtgca
     6901 acgctaaacc gaccactcca gtgaacgtca gttttttcag gcattgcgct ggtttggttg
     6961 attttttgca tttcagaatt accgtacatt ttcaaatgta gaaattattt tatcgatata
     7021 tcatggggtt atgttattca gcatcactgt tcaggaggct caatagcggg atactatacc
     7081 ataacaacag gaagcgcctg tctcattgca aaagaaaatt gagatcctct caaggcatga
     7141 agctctcacg aagtgatgga aataatctta ttagccgtta gctttgttaa ggccaatgat
     7201 aaacaatcca ggttcgacga taaataaaaa tcacacatta aactccggtg atatctctcc
     7261 ctgctaatag cactgataga gaaaaaaaga acccaataag cattgggttc ttttatgtaa
     7321 tgccttccat accatcgaag aacttcacat attatttctc cgatttaacc ccgaataaat
     7381 cataaattaa tttagaagtg cctgtatata ttttaatctt ttcctttgag tttgggtcaa
     7441 acgacttagc aaagtcaatt aatttcggtg cagcatctcg cattttgctt aaaatatcag
     7501 gggcgagctt tccttcattc accagatgtg acatcttatc cagatagcca tcaaaattca
     7561 tttctccccc atctgccaga cgcttcatct ctcccaggta cttcttcata tcacgttgag
     7621 ataactcttc aaactgagct gtcagatagt tctcattatc atcattaata ttcacttttt
     7681 ctgctcctgc taaatctgtg taggcactag agtatgaaag tgaaatcaac agcgaagcga
     7741 ataattttaa gctgttgtca tcatatttct gacatgcatt aacaaacgtt aaaaaaccag
     7801 aacctattgc ttgtaaatgt atatttgcta aaggttcatt atctttagat tcttcataaa
     7861 ataactgaac cgtggaggga aggccat
//
LOCUS       DAZGBC010000195         7779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGBC010000195 DAZGBC010000000
VERSION     DAZGBC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.196"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(154..417)
                     /gene="cedA"
                     /locus_tag="ACHK1J_004400"
     CDS             complement(154..417)
                     /gene="cedA"
                     /locus_tag="ACHK1J_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO7913586.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            600..2861
                     /gene="katE"
                     /locus_tag="ACHK1J_004401"
     CDS             600..2861
                     /gene="katE"
                     /locus_tag="ACHK1J_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO7913587.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAIILNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2908..3666)
                     /gene="chbG"
                     /locus_tag="ACHK1J_004402"
     CDS             complement(2908..3666)
                     /gene="chbG"
                     /locus_tag="ACHK1J_004402"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO7913588.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGVVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3679..5031)
                     /gene="celF"
                     /locus_tag="ACHK1J_004403"
                     /gene_synonym="chbF"
     CDS             complement(3679..5031)
                     /gene="celF"
                     /locus_tag="ACHK1J_004403"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO7913589.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5135..5977)
                     /gene="chbR"
                     /locus_tag="ACHK1J_004404"
     CDS             complement(5135..5977)
                     /gene="chbR"
                     /locus_tag="ACHK1J_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO7913590.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5985..6335)
                     /gene="chbA"
                     /locus_tag="ACHK1J_004405"
     CDS             complement(5985..6335)
                     /gene="chbA"
                     /locus_tag="ACHK1J_004405"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO7913591.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6386..7744)
                     /gene="chbC"
                     /locus_tag="ACHK1J_004406"
     CDS             complement(6386..7744)
                     /gene="chbC"
                     /locus_tag="ACHK1J_004406"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO7913592.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2109 a         2027 c         1731 g         1912 t
ORIGIN      
        1 aataaataaa caattaaaga aaaaagatca cttatttata gcaatagatc gtcaaaggca
       61 gctttttgtt acaggtggtt tgaatgaatg tagcaatgaa atacaaaatt tcaggccatg
      121 taactcccgg caaaaccggg aggtacgtaa tccttactca gtcacttccc cttcctggcg
      181 gatctgattt gcccaacgct gggcggattc aggcacagta aacgccggtg agcgcagaaa
      241 tgactctccc atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa
      301 cgaatccatc tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat
      361 aatctggcgg ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt
      421 aaaaatcaga aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta
      481 tttcataaca ccgtttccag aatagtctcc gaagcggggt ctggctggtg gtctatagtt
      541 agagagtttt ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa
      601 tgtcgcaaca aaacgaaaag aacccacatc agcaccagtc accactacac gattccagcg
      661 aagcgaaacc ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg
      721 aaccaacacc gccaggtgca caacctaccg ccccagggag cctgaaagcc cctgatacgc
      781 gtaacgaaaa acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga
      841 ccactaatca gggtgtgcgc atcgccgacg atcaaaactc actgcgtgcc ggtagccgtg
      901 gtccaacgct gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc
      961 gcatcccgga acgtattgtt catgcacgtg gatcagcagc tcacggttat ttccagccat
     1021 ataaaagctt aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc
     1081 cagtatttgt taggttctct accgttcagg gtggtgctgg ctctgccgat accgtgcgtg
     1141 atatccgtgg ctttgccacc aaattctata ccgaagaggg tatttttgac cttgttggca
     1201 ataacacgcc aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg
     1261 taaaaccaga accacactgg gcaattccac aagggcaaag cgcccacgat actttctggg
     1321 attatgtttc tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg
     1381 gcatcccccg cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta
     1441 atgccgaagg gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct
     1501 cactcgtttg ggatgaagca caaaaactaa ccggacgtga cccggacttc caccgccgcg
     1561 agttgtggga agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga
     1621 ttcctgaaga agatgaattc aagttcgact tcgatcttct cgatccaacc aaacttatcc
     1681 cggaagaact ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aacccggata
     1741 acttctttgc tgaaaacgaa caggcggctt tccatcctgg gcatatcgtg ccgggactgg
     1801 acttcaccaa cgatccgctg ttgcagggac gtttgttctc ctataccgat acacaaatca
     1861 gtcgtcttgg tgggccgaat ttccatgaga ttccgattaa ccgtccgacc tgcccttacc
     1921 acaacttcca gcgtgatggc atgcatcgta tggggatcga cactaacccg gcgaattacg
     1981 aaccgaactc gatcaacgat aactggccgc gcgaaacacc gccgggaccg aaacgcggcg
     2041 gttttgaatc atatcaggaa cgcgtggaag gaaataaagt tcgcgagcgc agtccatctt
     2101 ttggcgaata ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc
     2161 gtcatattgt cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg
     2221 agcgcgttgt tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa
     2281 atctcggtat cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg
     2341 gtctgaaaaa ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc
     2401 gcgtggtagc gattatactt aatgatgaag tgagatcggc agaccttctg gccattctca
     2461 aggccctgaa ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga
     2521 ctgcggatga cggtacggtg ttgcctatag ccgctacctt tgccggtgcg ccttcgctga
     2581 cggtcgatgc ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg
     2641 ccaactacta cctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg
     2701 cgcgcaagtt taaagcaaca atcaaggtcg ctgaccaggg tgaagaaggg attgtggaag
     2761 ctgacagcgc cgacggtagt tttatggatg aactgttaac gctgatggca gcacaccgcg
     2821 tgtggtcacg cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc
     2881 ccctggggag tgaccttacc accttactta cacatcaaga taactaccca accgataacc
     2941 acgctcagca attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg
     3001 tgggaaacag taagcactct ggcgaatcgt attatcgaca aacgccggat ggcacatgac
     3061 ttccagcgag cgttccccgc gatggctgga atcatcaagc acttgcagga acagtgcctc
     3121 gctaatctct tcaccataaa acgcgctact gaatccgtgc gaactgcgca gattagccgg
     3181 taaatcacca gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc
     3241 cgcaaacctg gcaacaatag ggaaaatctg cgggaacata tgcacatgat gatggctatc
     3301 aagatgcgta ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc
     3361 ctgagtaatt tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc
     3421 cagcacacca tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac
     3481 aaagtgcatc cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat
     3541 agcctgccca ttcaccagcg ccgtcgtcga tgtgacaacc ccattgcgac aagcctcgat
     3601 aatgccgtag ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg
     3661 ttccataatc agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt
     3721 tggcagccat ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc
     3781 ggaatgcacc aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag
     3841 tgcggcgttg ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt
     3901 atcatcgaaa tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcgtacaggt
     3961 catttctacc gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac
     4021 gtaatgttct gcttgcttat cgttgtagat agcgttgatc acttcgcacg ctgcatcaga
     4081 gtaataagcc ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt
     4141 atacagctca aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt
     4201 gtagtattcg cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata
     4261 agaacacggc aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat
     4321 atttttaacg ccagatgctt ttaactgccc ggatgccaca ccatcaagta attcggcaaa
     4381 gcgagatttg ccatttacca gcacatcctt aatgaacacc atatggttga ggccgaacag
     4441 atcgatagat aaatcatcgc tgtctttcag catcagaaca tcgcgaataa acatcttcat
     4501 gccgatcgga atattacaca cgccgataaa gcgtttaaat ccggtatgac gataaacggc
     4561 ttcagtgacc attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc
     4621 ttcgacatct tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacagacc
     4681 gcccgcgccg ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag
     4741 ttcgcgcgcc ggtaattggc ctacgcgcag ttgggtagta acgaaatcag catctttcaa
     4801 tgcttcgcgg cgatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat
     4861 ccgttggcag agatcgaaaa taatatccag tttagctttg ccaccttcga catccaccag
     4921 ccataattcg ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg
     4981 ggtatagctg ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc
     5041 cttcagtaca gaatactgat atctgacata tctgcccccc ggacataaat aatccagcaa
     5101 caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct
     5161 tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg
     5221 ggctactata accggcctca aacgcaatat ccgtcactga atagttggtc atttccagtt
     5281 gttttttggc aaaattaata cggatttcat taataatctg cattggcgtt ttgccataat
     5341 atcgttgagt cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct
     5401 ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc
     5461 actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat
     5521 aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc aatgaagaaa
     5581 tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagctacaa
     5641 agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt
     5701 tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa
     5761 aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc
     5821 cggttaatac cagagtaaat tcataatagt cgtgctgatg cagtccgctg atactctcag
     5881 ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag
     5941 tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt
     6001 tcatgaagct caattaattc tgtaatcagt tcacgcgcaa gcatggacgt cattaaatga
     6061 tcctgagcgt ggaccagcac cagactcact ttcatcttac cttcgcccgc atcgccttca
     6121 atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg
     6181 gcttttgctg cggcaaaatc gccctgcttc gcctgtttca gtgccgcata cgccaggctg
     6241 cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc ttcagcttcc
     6301 gtttgcgtat cgggaatatt atcgagatcc atcatatatc gttcctctct attcttaccg
     6361 gcacgattac ccgtaccggc atcgattaaa atttcagggc gttagcgata tcttcttcgc
     6421 tctcttcttt atcaatcgcg ttttgtgctt tgttagccac cacaacaaag ggcagataaa
     6481 ttaacgttgc gataccaagg ttgaagagtg caaccagcaa tgcggcgacg ctaccgttgg
     6541 tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag
     6601 gaataatgcc catgtagtac gctgccaggg tgattgccgc cagaatcggt tgtaccagta
     6661 caaacgggat aaacatcacc gggttcataa taattggcag accaaacaga atcggttcgt
     6721 taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc
     6781 gagaggcgat aaaaatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa
     6841 taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg
     6901 cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa
     6961 tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg
     7021 gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga
     7081 tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa
     7141 tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa cgcgatactg
     7201 aagcaggtac gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca
     7261 tttctgccac caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt
     7321 ttgcgccaac cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa
     7381 cggataacaa cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa
     7441 taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg ttgccgccaa
     7501 tacctttcag accattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat
     7561 aaaaaaacga cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc
     7621 ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct
     7681 gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac
     7741 tcatagaaaa atacccttaa aaaccgcaat ttaaatatt
//
LOCUS       DAZGBC010000196         7744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGBC010000196 DAZGBC010000000
VERSION     DAZGBC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.197"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..400
                     /locus_tag="ACHK1J_004407"
     CDS             <1..400
                     /locus_tag="ACHK1J_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913593.1"
                     /translation="NPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLLSIP
                     ISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNN
                     LGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            444..3666
                     /locus_tag="ACHK1J_004408"
                     /pseudo
     CDS             444..3666
                     /locus_tag="ACHK1J_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            4093..5748
                     /gene="lldP"
                     /locus_tag="ACHK1J_004409"
     CDS             4093..5748
                     /gene="lldP"
                     /locus_tag="ACHK1J_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO7913594.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            5748..6524
                     /gene="lldR"
                     /locus_tag="ACHK1J_004410"
     CDS             5748..6524
                     /gene="lldR"
                     /locus_tag="ACHK1J_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO7913595.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            6521..7711
                     /gene="lldD"
                     /locus_tag="ACHK1J_004411"
     CDS             6521..7711
                     /gene="lldD"
                     /locus_tag="ACHK1J_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO7913596.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1839 a         1911 c         2119 g         1875 t
ORIGIN      
        1 taatccggca aaaacgaagt atttcatcct gaacaatggt tcggtggctt tagcgggccg
       61 agtgttatcc attgacgcaa cagagaatgg cagtgttatt catatttcac tggtcaactt
      121 attaagtata ccaatctcaa acattggttt taatgcgaca tggggtggtg aaaaacctgt
      181 cgatgccaaa gagtttgccc ggtggcaaca attacttttc aacacatcaa tgaaatccac
      241 attgaaatta ttaccaggtc aatggcaaga cattaatttg acactgaagg gtgtatcgcc
      301 gaataacctg ggatatctga agttagccat caatatggag aatattcagt ttgacaacct
      361 tccctctgct gaaaatcggc agaaaagaag caaaaaataa atgcagcttc tgcgcttata
      421 tcaaggaata gagaacatca ttaatgaaca aaatatttaa agttatctgg aaccctgcga
      481 cagggaatta tactgttacc agcgaaacgg caaaaagccg tggcaagaaa tccgggcgca
      541 gtaagctgtt aatttctgcc gtggttgcgg gtgggttgtt gtcgtcgttt gaggcactgg
      601 caacaggtaa tgaacctggt acacctgttg gcacaggatc tggctgggtt gctataggcc
      661 aggaatcaca ggcaaatgct tttaccgatg gtaatggtgc cagtactgca gtgggttata
      721 aagctttcgc tgaaggaaca tggagttccg ctattggcgc catgacccat gcaattggtc
      781 cggcttcaat ggcattcggg gttaatgccg tctcaaccgg tcaacgaagt atcgctatgg
      841 gcgcatcgtc aagttcagtg gtcaatattc gatggcactt gggcgatatg cgaattcggg
      901 tggtgattat tcgatcgcgc aaggggataa atccaatgct gctggtctga atgccattgc
      961 agtgggaaga agcagtagtg cggcaggcga gaaagccact gcgctgggca acgcagctaa
     1021 agctactgag attatgagta ttgctcttgg cgacagcgcc aatgcgtcaa aagcgtattc
     1081 aatggcgctg gtagcaagta gcgtcgcatc tgaagaaaac gcaattgccc tggggcgtag
     1141 cagttagcta gcggtactga cagcctcgca ttaggcagac aaacacttgc cagtacagcg
     1201 aacgctattg cgataggtgc tgagagcaaa gccgctgaaa atgcgacggc cgttggtacc
     1261 aaagcggagg ccaatggact taatagtatc gcgttgggct ctgggggtat tgcagatgcc
     1321 gataacacga ttgctctggg caatcaaagc ctggcagtag ctgctggtgc aattgccatc
     1381 ggtcagggga ataaagctga cggcgcaaat gctatcgcgc tgggtaatgg tagtgttaca
     1441 ggtggcgcaa atgctattgc tcttggacaa ggtagttatg ccggtttaga aaatggcatt
     1501 gcaattggtg gtcaagccag tgctcaaggg acaaattccg ttgctctggg tgcgggttct
     1561 gtagcgactg aagataattc cgtctctgtg ggtaacacaa cagatcagcg aaaaattgtc
     1621 aatatggcag caggtgatat cagcactagc agtactgatg ccataaatgg atcacagctt
     1681 tatgctatca gtaagtcagt agcggaccgt cttggtgggg gggctaccgt caatgcgcaa
     1741 ggtgtcgtta ctgccccgaa ttacagactg aaaaatggta tttatggcaa tgttggcgat
     1801 gctttagcgg atctaaacaa taacacgata caatgggact ccgttaaaaa gggatatagt
     1861 gcggcacatg gtacagatgc caccagcacc atcaccaacg ttaaagccgg cactatttct
     1921 gctaccagta cggatgcggt taacggttct cagttgaatg cgacgaatat gatgattgag
     1981 cagaacagcc aaatcatcaa tcagcttgct ggtaacaccg acgcaaccta tatcgaagaa
     2041 aatggcgctg gtatcaacta cgttcgtact aacgacaacg gtttagcctt taacgatgca
     2101 agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc tggtgccagc
     2161 agcgtagcca ttggtcagaa cagcaatagc accgttgata ccggtattgc gctgggtagc
     2221 agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt aacggaaaat
     2281 ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt gtcaattggt
     2341 aatgacggta aataccgcca aatcatcaac gtagctgatg gttccgaagc ccatgacgcc
     2401 gttacggttc gtcaattgca gaatgcgatt ggtgcggtcg caaccacgcc aaccaagtac
     2461 tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc gctggcaatg
     2521 ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa cactctggtt
     2581 ctggctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa ccatgcaaac
     2641 agtattgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg ctacaccgcc
     2701 tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgttggtag tgaagacggt
     2761 caacgtcaga tcaccaacgt cgcagctggt tcagcggata ccgatgcggt caacgtaggt
     2821 cagttgaaag taacggatgc gcaggtttcc cagaataccc agagcattac taacctgaac
     2881 aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat tggcgatatc
     2941 gtcaccaccg gtagcatcaa gtacttcaag accaataccg atggtgtaga tgccagcgcg
     3001 cagggtaaag atagcgtcgc gattggttct ggttccattg ctgccgcgga taacagcgtc
     3061 gcactgggta cagggtctgt ggcaaccgaa gaaaatacga tctctgtagg ctcatctact
     3121 aatcaacgtc gtatcaccaa cgtagccgca ggtaaaaacg acaccgatgc tgttaacgtg
     3181 gcacagttga agtcttctga ggcgggcggt gtacgttacg acaccaacgc tgatggttct
     3241 gttgactata gcaatatcac cctcggtggg ggcaacggcg gtacgactcg tatcagcaac
     3301 gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa gcaaagcgtg
     3361 caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa actgtctaaa
     3421 actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatggcaat gaccggtctg
     3481 ccgcaggctt acactccagg tgccagcatg gcctctattg gtggcggtac ttacaacggt
     3541 gaatcggctg ttgctttagg tgtatcgatg gtgagcgcca atggtcgttg ggtctacaaa
     3601 ttacaaggta gtaccaatag ccagggtgaa tactccgccg cactcggtgc cggtattcag
     3661 tggtaatgat ccattaagtt agtgtgacta agggcaaacg ccaaattgcc tgatgcgcta
     3721 cgcttatcag gcctacaggg tgattctgca cattcctata ggccgggtaa ggtgttcacg
     3781 ccgcatctgg caagataagg cgctccgggt caacaatcta agggcaattc tctgatgagg
     3841 attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc aattcttcgc
     3901 ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatgtt gccaatacat
     3961 aacatttagt taactattca ttgtcattat ccctacacaa cacaattggc agtgccactt
     4021 ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg cgtggactcc
     4081 aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa tatctggctt
     4141 tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat taagctcaaa
     4201 ctgaaaggat acgtcgccgc ctcatggacg gtggcaatcg cccttgccgt ggctttgctg
     4261 ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt cttctacggg
     4321 ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat ctcggtgaaa
     4381 accgggcagt ttgacatcat tcgctcgtct attctttcga taaccccaga ccagcgcctg
     4441 caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc aggctttggc
     4501 gcaccggtag caattaccgc cgcattgctg gtcggtctgg gctttaaacc actgtacgcc
     4561 gccgggctgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat gggcattcca
     4621 atcctggttg ccggacaggt aacaggtatc gacagctttg agattggtca gatggtgggg
     4681 cggcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat tatggacggc
     4741 tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg cgggcggctc gtttgccatc
     4801 gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat ctcttcgctg
     4861 gtatcactgc tctgcctgac gctgttcctc aaacgctggc agccagtgcg cgtattccgt
     4921 tttggtgatt tgggggcgtc acaggttgat atgacgctgg cccacaccgg ttacactgcg
     4981 ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt aacactgtgg
     5041 agtatcccgc cgtttaaagc cctgttcgca tcgggtggcg cgctgtatga gtgggtgatc
     5101 aatattccgg tgccgtacct cgataaactg gttgcccgta tgccgccagt ggtcagcgag
     5161 gctacagcct atgccgccgt gtttaagttc gactggttct ctgccaccgg taccgccatt
     5221 ctgtttgctg cgctgctctc ggttgtctgg ctgaagatga aaccgtctga cgctatcagc
     5281 accttcggca gcacgctgaa agaactggct ctgcccatct actccatcgg tatggtgctg
     5341 gcattcgcct ttatttcgaa ctattccgga ctgtcatcaa cactggcgct ggcactggcg
     5401 cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg ggtattcctg
     5461 accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa
     5521 caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg cgtcaccggt
     5581 aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa
     5641 gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg tatggtgggc
     5701 gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg attgttttac
     5761 ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc
     5821 tggaagcggg catgaagttg cccgctgaac gtcaactggc gatgcaactc ggcgtatcac
     5881 gtaattcact gcgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac
     5941 gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc
     6001 agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt ctggaagcac
     6061 gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca cctggcgaca
     6121 aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat atcgcctcac
     6181 aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc gtgctgctgc
     6241 aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga
     6301 tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct gtcattgacg
     6361 ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac cttagttttg
     6421 ttcacaccac aatgaaacga ttcgatgaag atcaggctcg ccacgcacgg attacccgcc
     6481 tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt ccgcagccag
     6541 cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact atatggatgg
     6601 gggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag aagtggcgct
     6661 gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc tgtttaatga
     6721 gaaattgtcg atgccggtgg cgctaggtcc ggtaggtttg tgtggcatgt atgcgcgacg
     6781 cggcgaagtt caggcagcca aagcggcgga tgcgcatggc attccgttta ctctctcgac
     6841 ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa tgtggttcca
     6901 gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag caaaagcagc
     6961 gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggagcgc gttatcgtga
     7021 tgcgcattct ggaatgagcg gcccgaacgc ggcaatgcgc cgctatttgc aggcggtgac
     7081 gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccgcatgatt taggaaatat
     7141 ttcggcttac ctcggcaaac cgaccggact ggaagattac atcggctggc tggggaataa
     7201 cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct gggatggccc
     7261 gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac gttttggtgc
     7321 tgatgggatt gtagtttcta accacggtgg ccgccagctg gacggtgtac tctcttccgc
     7381 tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atagccattc tggcggatag
     7441 cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact
     7501 gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg tagctaacct
     7561 gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg cgaaatcgat
     7621 cagcgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc ctgcggcact
     7681 ggctcccatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcccc ctaaccctct
     7741 ccct
//
LOCUS       DAZGBC010000197         7681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGBC010000197 DAZGBC010000000
VERSION     DAZGBC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.198"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..777
                     /gene="gadX"
                     /locus_tag="ACHK1J_004412"
     CDS             <1..777
                     /gene="gadX"
                     /locus_tag="ACHK1J_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO7913597.1"
                     /translation="YILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLS
                     LFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALI
                     FALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLK
                     KKLREEETSYSQLLTECRMKRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGM
                     TPTEYQERSAQRLPNRDSAASIVAQGNFYGADCSAEGIRL"
     gene            1145..1873
                     /gene="gadW"
                     /locus_tag="ACHK1J_004413"
     CDS             1145..1873
                     /gene="gadW"
                     /locus_tag="ACHK1J_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO7913598.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2236..5349)
                     /gene="mdtF"
                     /locus_tag="ACHK1J_004414"
     CDS             complement(2236..5349)
                     /gene="mdtF"
                     /locus_tag="ACHK1J_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO7913599.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGADDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5374..6483)
                     /gene="mdtE"
                     /locus_tag="ACHK1J_004415"
     CDS             complement(5374..6483)
                     /gene="mdtE"
                     /locus_tag="ACHK1J_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO7913600.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6591..6869
                     /locus_tag="ACHK1J_004416"
     CDS             6591..6869
                     /locus_tag="ACHK1J_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913601.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6870..7397)
                     /gene="gadE"
                     /locus_tag="ACHK1J_004417"
     CDS             complement(6870..7397)
                     /gene="gadE"
                     /locus_tag="ACHK1J_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO7913602.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1976 a         1800 c         1837 g         2068 t
ORIGIN      
        1 tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga cctggttttt
       61 gcggatgcaa gccaaattcg agtagataag tgtgttgaaa attttgtatt agtatcaagg
      121 gatacgcttt cattatttct cccgatgctc aaggaggagg cattaaatct tcatgcacat
      181 aaaaaagttt cttcattact cgttcatcac tgtagcagag atattcctgt ttttcaggaa
      241 gttgcgcaac tatcgcagaa taagaatctt cgctatgcag aaatgctacg taaaagagca
      301 ttaatctttg cgttgttatc tgtttttctt gaggatgagc actttatacc gctgcttctg
      361 aacgttttac aaccgaacat gcgaacacga gtttgtacgg ttatcaataa taatatcgcc
      421 catgagtgga cactagcccg aatcgccagc gagctgttga tgagtccaag tctgttaaag
      481 aaaaaattgc gcgaagaaga gacatcatat tcacagttgc ttactgagtg tagaatgaaa
      541 cgtgctttgc aacttattgt tatacatggt ttttcaatta agcgagttgc agtatcctgt
      601 ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa attattatgg gatgacgccc
      661 acagagtatc aggagcgatc ggcgcagaga ttgccgaacc gtgactcggc ggcaagtatt
      721 gttgcgcaag ggaattttta cggcgctgac tgttctgcgg aaggaataag attatagagt
      781 tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag tctgccgtct
      841 ccagactaat aaaccgttat aacactccct gttggcacgg gaaactttgt gctctcagta
      901 agttaaatat aacttttact ggaaataaga tcagccattt ttttataaac ataagctata
      961 cgctgtgcga aaatatattc gtgctgcatt tacttattat caattaactg ttatgcaaaa
     1021 ctactttgtg gataaatttt ggtcctacca aatctggcag tttttgcgct aagaaacagt
     1081 ctggcatcat ttcattagta tactgaaatt gaaataatcg cagtatgaaa tataagggat
     1141 aatcatgact catgtctgct cggtgatcct cattcgtcgt tcattcgata tttatcatga
     1201 acagcaaaaa atatcgctgc ataacgagag tatcctgctg ctggagaaaa atttggcaga
     1261 cgattttgcg ttttgttcac cggatacgcg acggctggat atcgatgagc tgacagtttg
     1321 ccattactta caaaatattc gtcagctacc acgcaattta gggttacata gcaaagaccg
     1381 tttgttaatt aaccagtcac cccccatgcc gctggtgacg gcgatttttg atagtttcaa
     1441 tgactccggg gtaaattcgc cgatactgag caaaatgctc tatctttcct gtttatcgat
     1501 gttttctcat aagaaagaac tgatcccctt acttttcaat agtatcagta ctgtttcagg
     1561 aaaagttgaa cgccttatta gctttgatat cgccaaacgt tggtatctgc gcgatatcgc
     1621 ggaaagaatg tataccagcg agagtctaat caaaaaaaag ttgcaggatg aaaatacctg
     1681 tttcagtaaa atattactcg cttccaggat gtcgatggcc agacgattac tcgagttacg
     1741 tcaaattcct ctgcatacta ttgcggaaaa atgtggctat agcagtacgt cgtactttat
     1801 aaacacattt cgacaatatt atggtgtaac gccacatcag tttgcgcaac attcgccagg
     1861 taccttttcc tgacatattt tgcatttgaa tattggtcag gatctcacac ctgcttcatg
     1921 tgaaactctt ccctgatgat ttctgccggg ctaccgacta gttctctttc gcagtttgcc
     1981 aggcggtata ggtattttca ggaggaaaag actcggcatg tttgggatta ttaagctgac
     2041 aattcatacg attacgggaa tgtgggtcag tattgtgctg ttcaaattga tgacgaatgg
     2101 ctggagtggt ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg
     2161 gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct gaaccttcac gttcaacctt
     2221 actctcattt acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa
     2281 aaagaccgga acaaagtaaa ttgccagcac tgttgcggca aacatcccgc ccatcacgcc
     2341 ggtacctacc gcgttttgcg cgccagaacc ggcaccatga ctgataacca gcggcagcac
     2401 gccaagaata aaggccagag aggtcatcag gattgggcgt aaacgcattc gtgccgcttc
     2461 gatgattgcc tctatcggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac
     2521 aatcaggatg gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta
     2581 gacgtcatta cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg
     2641 aacaaccaac atcaccgaga acggaattga ccagctctca taaagtgcgg cgagggcgag
     2701 gaacaccacg accagtgaaa tggcatatag cgcgggagcc tgatttgagg ataacgcttc
     2761 ctgatacgac agtccggtcc atgagtagcc gacgcctgcc ggaagtttag cgaccaggtc
     2821 tgccataaat ttcattgcgt caccggtact tttcccggcc gccgcttcac ctaaaatctc
     2881 cattgacggg atgccgttgt agcgttccag tcgcggtgaa ccataggtcc attcggtaga
     2941 cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat
     3001 gttatccggc aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc
     3061 ctggttgagg aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat
     3121 atcagacagc gccacaccca ttgcctcagc tttcgcggcg ttgacgttca ctttgaacat
     3181 cggcgtatct tccaggccgt tcgggcgtac cccggtgacc tgattcggtg attgcgctgc
     3241 cagtgataac agctcgtttc gtgcctgggt tagtttttcg tgccccaggt taccgttgtc
     3301 cagcagttcc atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa
     3361 cgggaagacg acggctttat tgatactgct tagcgcaatc attgcccgct gaatgatcgc
     3421 ggtaaccgag ttttcctcac cgacacgttc agaccacggc ttgagactga taaacgccag
     3481 gccgttgttt tgcccctggc cgctgaagcc aaagccgcca acggtaaaca ccgactggac
     3541 attatctttc tctttagtca gataataatc cgtcacctgt tgcagcactt tcgtggtgtt
     3601 aaccatggtg gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc
     3661 tggtaagaaa gaggtcggcg tgcgcaggaa cagcaccgcc atcccggcgc aaatcaggag
     3721 gtagaccacc atgtagcgac cggtacaacg caacagtgag cgggtgctat cggtatagtg
     3781 ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgacc
     3841 gccttccggc gcggctttca gaatggtagc gcacagggca ggggtcaggc tcattgccac
     3901 aaatactgaa agcagcatgg aggagatcag cgtgatggaa aactggcggt agatctcccc
     3961 ggttgcaccg ctcataaagg ccatcggcat aaacactgcg gaaagaacaa cggcaatacc
     4021 gaccagcgca cgttggatct gccccatcga tttatgcgtc gcttccttcg gcggtagctt
     4081 atcttccgca atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa
     4141 ccctatcgcc agcaccatcc cgaacatagt caaagtgttg atggtgaaac cgaccgccga
     4201 caagatcgca aacgtcccga gaataaccac cggtacggca atcgtcggga tgattgtggc
     4261 acggaaattc tgcaaaaaca gatacatgac caggaagacg aggatgatag cctcaaccag
     4321 tgttttgaaa acttcctgaa tagaaatttc gataaacggc gtggtgtcgt aaggataaac
     4381 cgtcttcaga cttgccggga aataggctga taagcggttc agttcctctt tgactgcccg
     4441 cgaggtatcc agggcgtttg ctccggtagc cagtttgatg gcgatcccgg cagcaggttt
     4501 gccgttatag cgcgccacgg tggaataatc gtccgcccca agttcgacgc gggcgacatc
     4561 acgcagcagc acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc
     4621 cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt agttgctggt ctgccgcctg
     4681 tggcatgcca cccagttgac caccagaaat ctggttgttt tgcaccttaa tctgggaaat
     4741 aacatcggaa ggtaccagat tgtatttatt gagtttttgc gggtccagcc agatacgcat
     4801 ggcgtactcg gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc
     4861 tttgatatta gacgctacat agtccgcgat atcgtactgg tttaggctgc cgttatcaga
     4921 aataaacgcc gctaccatca ggatattact gctcgattta tcgacgctaa tcccctgctg
     4981 ctgcactgct tcaggtaatg aaggcatagc gagttgcagt ttattttgca cctgaacctg
     5041 tgcgatatca ggagatgtcc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc
     5101 atcactggtt gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac
     5161 ctgagtgacc gagtcttcta ccgtttgcgc atcggcaccc ggataggtag cgctgacggt
     5221 aatggtcggt ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc
     5281 acctgcaagc atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata
     5341 gttagccatg aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt
     5401 ttcctggctg gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga
     5461 aacgatcacc cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc
     5521 tttgctggct tcaatttcgc gtagctgcac gacatcgtct ttatccagaa tgagcgccgt
     5581 tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc accagtaata cattctggcg
     5641 gctaccttca tccactaatg ccgtgacata catgccaggc agcaagtcac catttgggtt
     5701 ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca tcaaccgtcg ggtcggagaa
     5761 tttcagcgtg ccggtctggc tgtaacgttt accattttcc agattgagct gtactggcgt
     5821 actgccctga acctgtttga tttgcccact ggcgacctct tctttcatgc gtaagaaatc
     5881 ctgcaccgac tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga
     5941 atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc
     6001 cgtaatcggc gaggtgacat tcgcgtattg cagattgatc gtcgcctgtt caacagccgc
     6061 tttggcgacg gtgacattgg cttctgcttc attcaactgg gtgcgcgcgg tgtcgtaatc
     6121 ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc
     6181 attgctggcg gtagagagcg ctttcgccag cgagcctttg gcggagttta gctcggcctg
     6241 taaaggtgca ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat
     6301 aaagttgcgt ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg
     6361 aacggttcta ccgggcaatt cgctcaacac attgaccgac ccaggggaga gtgtgacgac
     6421 accgacctca ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag
     6481 catcgcgccg cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc
     6541 tgaaaattct tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt
     6601 tacagaccag cggcatagtg gggttgtcag ccagtaaggt gggatacagg catagtgatc
     6661 gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca
     6721 ttcgggcaac gaagggttct actggtggat acacatacca ggggaataaa gtcgttttag
     6781 cctctccttt agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca
     6841 gccatcaatt tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg
     6901 atgtcgctca tacgtcttag tttcagcttg tacataatgt tgaatttctg aatctttaat
     6961 gtttgttgcg aaatattaag cgccgacgtg atattgcttt catttttatt tttcagaatc
     7021 agttgcataa ttttgaactg attatgtgaa aaacataatt gtgaattctt atggggcaag
     7081 tgtttaccat aagtaatctc aagaagattt ttgatacttt ctttgcggct aacaaaataa
     7141 ataggcgctt tagcttttag tttactgatg tgataaggtg ccaaaacaat aattctctcg
     7201 gcatctaatt tctccagaaa tttaatcgct tcttcatcaa ggatatgatt gtgataggta
     7261 tcaatgataa ccttgaaggg tttattgcct acttttttga tctctgacag ggaattgatt
     7321 tttatcattt cgtgattatc tttcaactgc caaaagccct gtaaaagaaa agaatctttc
     7381 gtcatgagaa aaatcataac ttgctcctta gccgttatcg tttcgaacat gtcatccttt
     7441 tcattattta catccttgtc cgaatcgttg ttcaatatag taaacgccta taagaacttt
     7501 gcccttcgtg aactaatacg tctattaaca ctaatcaata cttcagattt atccatcacg
     7561 cactccatat attgatccta agtaagattc ctggttgtta tcagcttgta aatagcaaac
     7621 aaaaagttaa cgtttgcgga ttactgccca agaataagtt atcaataacc agtttgtgat
     7681 c
//
LOCUS       DAZGBC010000198         7630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGBC010000198 DAZGBC010000000
VERSION     DAZGBC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.199"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..553)
                     /gene="reiD"
                     /locus_tag="ACHK1J_004418"
     CDS             complement(<1..553)
                     /gene="reiD"
                     /locus_tag="ACHK1J_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFO7913603.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRHKNL
                     LDSIKAYIDHNFYEPIRLDALATK"
     gene            984..2489
                     /gene="iolA"
                     /locus_tag="ACHK1J_004419"
     CDS             984..2489
                     /gene="iolA"
                     /locus_tag="ACHK1J_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFO7913604.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
     gene            2514..3323
                     /gene="iolB"
                     /locus_tag="ACHK1J_004420"
     CDS             2514..3323
                     /gene="iolB"
                     /locus_tag="ACHK1J_004420"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFO7913605.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            3725..4035
                     /locus_tag="ACHK1J_004421"
                     /pseudo
     CDS             3725..4035
                     /locus_tag="ACHK1J_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4279..5703)
                     /locus_tag="ACHK1J_004422"
     CDS             complement(4279..5703)
                     /locus_tag="ACHK1J_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFO7913606.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIISSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
     gene            6142..7575
                     /gene="iolT1"
                     /locus_tag="ACHK1J_004423"
     CDS             6142..7575
                     /gene="iolT1"
                     /locus_tag="ACHK1J_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFO7913607.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT         2209 a         1763 c         1589 g         2069 t
ORIGIN      
        1 acttagtcgc cagagcatcc agacggatgg gttcatagaa attatggtca atataagctt
       61 ttattgaatc cagtaaattt ttatggcgcc gtgttatatc attacctgtg cgcattaata
      121 catttccctc gacgtattta agcagcaccc tggtcagcaa ctgcgccatt gttggtcctt
      181 ctactgtatg ctgatttata acattaaata ttgaactcat caatttctcg atcgtcgcat
      241 aatgatgata tagtggaatt acgggcacag tatcatctgg aatcagagca ttctcccgca
      301 gacccgggca cacaatattg tttatcccac agcaaatagt acccacaggc cgtccgcttt
      361 caagatactc atcatgcact acttcgctgt tataaataac caaatcacct tttctgactg
      421 ggtaaatgcg atcaccgatg aaatactccc cctttccctc ggtgataagg attagctcca
      481 cggtcctggg atgcatatgc atgaaccgta caaacgggcc attgctttcg ctaacctgac
      541 tgacatacgc caactgagcc cgggacttat tatcttgttc tgaaggacag aagcaaaaac
      601 gcatgatttt tcctccatta tccgaatctg tagtgctacg gccaaaacta aaactatttt
      661 tttataatat aaaaaaacag aataaaacgt tacatagatc atattatttt ctggtgaaac
      721 tcatctaaca cggataagat catcgaataa aacaatacag aatcacaatt tattttcttt
      781 gatattcaaa tagttaacaa caagcgacct ataaccatca ccccccatgc agtgaactta
      841 ttttttcata aatttaaatt tgaaatttat aatccgaagc tatacattaa acactgtgtg
      901 attttagact gtagatgatc aggctttgcc tgtgaacact cagtcggaac gtcgttaaca
      961 ttgattcaag aaggtaaaca gtgatggaaa cagttggaaa ttttattcag ggtaaaacga
     1021 gactgagcaa cagtggtgaa acactaccgg tgactaaccc tgcaaccgga gaagtgattc
     1081 gcctggttac ccagagcacc cggcaagata tgctggaggc gattcagagt gctcatgaag
     1141 cttttcctgc atggagtaaa atggcaccgt tgcgccgtgc aagaatcctt tttgaattta
     1201 aagtgttgct ggaaaaacac cgagatgaac tggcggctct gatcgtcagt gaacatggca
     1261 aagtatggtc cgacgcgctt ggtgaactga ctcgcggaat cgaagttgtt gaattcgcct
     1321 gcggcattcc gcatctcatt aaaggagagt tctcttttaa tgttggcaca ggcgttgaca
     1381 gcttttcatt aatgcaacca cttggtgttg tagctggcat cacacccttt aacttccctg
     1441 caatggtgcc aatgtggatg ttccctatcg ctctcgcctg cggcaatacc ttcgtcctga
     1501 agcctccagc gctggtgccc tccgcatcgt tacgtatggc gcaactacta caggaggccg
     1561 gactaccgga tggtgtattc aatgtagttc actgtggcaa tgacgcagcc agcctgttga
     1621 ccagtgatcc acgcattcag gccgtcagtt ttgtcggatc ttcttccgtc gctgagcata
     1681 tctacaccac cgccagtgca cacggtaagc gagtccaagc tttcggtgcc gcaaaaaacc
     1741 acgctattgt catgccggat gctgaccttg atgctaccgt taacgctatt atgggtgggg
     1801 cttttggttc cgcaggcgaa cgttgtatgg cattacctgt ggtggtcgct gtcggtgatg
     1861 aaacagccaa ccgtcttatt gaacgcctaa aaccacttat tgcagcgctg cgtattggcc
     1921 ccggtgagtt acgagaaaaa gacgaaaatg aaatggggcc agttgtttca cgtatgcatc
     1981 aacaaaaagt ccttgcatat atagacaaag gcgttagcga aggagcaaca ctggtgatgg
     2041 atgggcgaaa ttattgcgtg aagggctatc ctgaaggatt ttatgtcggc ggtaccttgt
     2101 ttgataacgt cacgccggaa atgactatct ggcgtgagga aatttttggt cccgtgctcg
     2161 gcatcgtacg cgtaacagat tatgccaccg ccattcgtac ggtaaactct catgaatttg
     2221 gcaatggtag cgtgatcttc acctccaacg gccattatgc ccgcgaattt gctcaatcgg
     2281 ttgaagccgg gatggtcggt atcaatgttc cggtaccggt tccaatggcg tttcactctt
     2341 tcggcggctg gaaacgctcg gtgtttgggg cgctgaatgt ccacggaccg gatggggtgc
     2401 gtttttatac ccggatgaaa accgttaccg cacgctggcc gaacggtcag cagatcgcat
     2461 ccgagttcag tatgcctacg ctaggttaat aaacgttcag gaaggagaag attatgacca
     2521 acttgctgag tacctgcacc accagggctg gaaatattca acatatttct ccgcaaaacg
     2581 caggctggga gtatgtcggt tttgatgtct ggctgttgaa agccggcgaa tctttaacct
     2641 tgccatccga tgatcgcgaa cgttgcctgg ttctggttgc cggacttgcc agcgtaaagg
     2701 ccgctgacca ctttttttat cggattggcc agcgcatgag tccttttgag cgaattccgg
     2761 cttattctgt ttacctcccg caccatacag aagcaaaagt cactgccgaa actgatctgg
     2821 aactggctgt ctgcagtgct cctggattcg gtgaacttcc ggtaagactg atctcaccac
     2881 aagatattgg ggtggaacat aggggaaaag ggcgcaatca gcgcttggtt cataatatcc
     2941 tcccagacac tcaacctgct gacagtctgc tggtagtgga agtatataca agggaaggcg
     3001 atacaagttc ctggcctgcc cataagcatg acacagcagt tgaagggcag gaaacctatc
     3061 tggaagaaac ctactaccac cgtttcgatc caccccaggg attcagctta cagcgagtct
     3121 acaccgccga tcgcagtctg gacgaatgta tggcagtata taaccgcgat gtggtaaaag
     3181 tcccgaaagg ctatcatccg gtggccacta tcgcaggtta cgataactat tatctgaatg
     3241 tgatggccgg gccattacgc aaatggcaat tcacatggga agctgatcac gcatggatta
     3301 attcgtcaga gtatccgcgt taacaatacg ttaaatattc ctttgccagt tgtcctctgc
     3361 agctggcttt tttgtgcaca gtccccaaca gggttctgcc tcttatacac aaatccacag
     3421 gcaacagaat cggtggattt ttttgaattt tttctctgtc tgataatatc ctgtttctgg
     3481 tgttctgtta tggttttttc tgattcttac tcctgagctg acaatatttt tggttctgcc
     3541 gaattaggag agaaatgccg tacacggcgg cttgttactc tggcttccta acgggctcaa
     3601 tacgttgggc agtctatcgt gaaatcatcc gtttctcagt tgaagttgaa gttgaagttg
     3661 aagttgaagt tgaagttgaa gttgaagttg aagttgaagt tgaaggagtc tatcgcagtt
     3721 agctctgaaa acatcaatgt ctcgtaaagg gaactgttac gacaaaaccc ctatggaaag
     3781 cttttgggga actctgaaga atgaaagtct gaaccactac cattttagta gccggaatga
     3841 agtcatctca gtaatactgg gtatacattg agatattcta caaccgtcag cgccgccact
     3901 cccgtctggg gaatatatcc ccggaaagct tcaggaaaat attatcaaat ggcagctaaa
     3961 aagaacaaat ggttgtgtcc accatgccag cacacctcac agaataaaag caccaacatg
     4021 aaaaagataa tatagccgaa tggttgttgc tcccatatca gagtaacctg tttatgaata
     4081 gaaattaatg aaatttaaca caaataccaa ataaaatacg tgtatcaaat gcaatcagaa
     4141 atatatttta tagttggtta taaaaaacct tctggatatt tattatctcc ttttgttacc
     4201 tatattaaca caaggaaaat cccggtaaga tgctcaacat taccgggcat tactgctgat
     4261 tgtgacacaa tcagactatt acatcatagc tttaccctga atttcacgca gtttatttcc
     4321 ggcaagctgc tcaatttgtg ttagcgggat cccctttgtt tcaggaacat agcgagaaat
     4381 aaaaacataa caaaccagat taaagacaac aaaaatccac atagaaaacg ctccgccaaa
     4441 ggtttcctgc agccaggtat tatcatttat taccggaaac aacagtgata ttagaaaatt
     4501 cgcaatccac atcaaactaa ccgccagccc catgccgaat cctttaatct tttcgggaaa
     4561 cacctcagaa atcagtaccc atgctcctac gctccagctg acagcaaaca acagcataaa
     4621 aaataaaatc ccaaaaatgg tgatatagcc agtatcatgg gtatacaaac catatgatgc
     4681 gatcaacaaa ccaataattg acccgatagt gccaatcttc atgattggta ttcgtccata
     4741 gcgatcgaac aaaatcatcc cgataaaact accgatcaca ttacaaacag caataaaaat
     4801 cgtctggaac atcacttcct ctgtactgct gttttgtagc accagaggtg cgtagtagtt
     4861 catcacattg acaccagtaa actgttgtaa aacagcgata caacagccaa ccagcagaat
     4921 atagcgcaac acaggagagc ttttcaggat aacgaattcc ctgcctgcag cattctcccg
     4981 ggcctccgtt tccctcatac tatcaaaaat agcggctaca tcggtagcgc taaccccccg
     5041 gtagattctg gcaagcactt tcagagaacg tttttccccc ttgttattac gggcacacca
     5101 tgccggcgat tcaggcaaaa ataaagttat cactaacatt gtaacagaag gtatcacctg
     5161 agcgaataac atgctcttcc agccttcttc gaccagcaca tcagagggca tccccttagc
     5221 aatcacataa ttaacaataa aaacaatcac ctgaccagaa actactgata tattgtaaat
     5281 acccagagct ttaccacgaa tgtcagctgg tgccagttca gacatataca tggtagatgc
     5341 agtccccgcc atcccaacag caaaaccact gacgatgcgc gaaatcgaga aaaccataaa
     5401 agattcactc atcgcccccc acagtgacga gatgataaaa ataactgcaa taatcagaag
     5461 cgcaggctta cgccccaact tatcggcaat tttccccccg accagcgcac caatgataca
     5521 accaatcaca atgctcgata ctgcccaccc agtttcagtt gaagtgagct ggtaatattg
     5581 ctgtaaaggc agtatcaccc ccgttatcac tgccgtcgaa tagccaaaca tcaatccagc
     5641 cagtgcagca acgcagcaca atgcgtacac ataggctgaa tcatacttgc ttctctgaaa
     5701 cataccccct ccctgcctgt tgaggaaatg acatacgaac atttattcca tacttaaaca
     5761 aatcagctca aatattccac aacaaagatc acaaaatata aaaatttcat aactgtgaaa
     5821 aatagtttgt agcacaataa tcatattgaa aatacagaac aataaaaaac tattattttt
     5881 catattgata aaagttacct gttgatgagt gagtatatat tcaccgagaa acacctagat
     5941 catgtaattt aaaatgtgat tcacataaca aaataaatgt ttcattattg atcttagtga
     6001 aaattaccaa tcagaatatt gaaatgtaat taccgatggt aaagcaagaa gtacaactgc
     6061 agagctacgg aaatgaccaa acacaatttt ttcattatag gtcaaataga tagatgattt
     6121 ctgctcagga gataatcagc tatgcccata tcagtgaatc gttacaatac aggctatata
     6181 ttacgtatct gttctattgc agcattaggc gggatattat ttggctacga tactgccgtt
     6241 atttcaggtg caatcagttc tctcaccagt tattttcacc tatctccggc cgaaaccggc
     6301 tgggcggttt ccagcgtcgt tgtaggttgt gtggtgggat cattcagctc aggttactta
     6361 tccaaacgtt ttggtcgcaa gaaatcactc atgatttccg ccctgctgtt cacaatctca
     6421 gcagtgggaa catctctttc ctacaccttc ctgcacttta ttatttatcg tatcgtaggt
     6481 ggacttgctg tcggcctcgc agcaacagta tctccaatgt atatgtcgga ggtttcacct
     6541 aaggacatgc gtggcagggc attgagtatg caacagtttg ccatcgtttt tggtcagatc
     6601 ctcatatttt acgtcaacta taaaatcgcc tcgattgcga ccaatacatg gctgattgag
     6661 attggctggc gttatatgtt tgctgccggc attgccccat gcattctgtt ctgtatttta
     6721 gtattcttta taccggaatc cccccgctgg atgatgatgg ctggtcgtga agaagaaacg
     6781 ctaaaaattc ttacaaagat atctaacgag gaacatgcac gacatttgct aacagatata
     6841 aaaacctctc tgcaaaatga ccggctgacg gcccaccaaa agctcaatta ccatgacagt
     6901 aacgtacgtt ttatcctttt cctcggttgc atgatcgcaa tgttacagca agtaaccggc
     6961 gttaacgtta tgatgtatta cgcacctatt gtattgagag atgttaccgg tagcgcccag
     7021 gaagcgctgt ttcagaccat ctggattggc gtaattcaac tcgtcggatc aattattggc
     7081 gcaatgatca tggacaaaat ggggcgttta tcgctaatgc gaaaaggcac cattggtagc
     7141 atcattggtt tattattaac ttcatgggct ctgtacagcc aaacaaccgg ctattatgct
     7201 ctctttggta tgctgttctt tatgattttc tatgcacttt cctggggcgt tggagcatgg
     7261 gtgctcatat cagaaatatt ccccaaccgc atgcgttcac agggaatgag tatttctgta
     7321 ggatttatgt ggatagcaaa ctttatggtt tcacagttct ttcctataat taacgaaaat
     7381 ccctggttac tggcccattt ccacggtgca tttccaatgt ggatattcgc agcctgctgt
     7441 gtctttagct atttctttat ttgccgctac ctgccggaaa ccaaaggtgt ctcactagag
     7501 aaaatggagg atatcgtgct gacaaaacgc aggaacaagc cacaacctat tcaggctgaa
     7561 cgctcttcag attaagctta gccaaaatgc cgatgatcat gaaaaacaga ttatcggcat
     7621 cttcctctta
//
LOCUS       DAZGBC010000199         7612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGBC010000199 DAZGBC010000000
VERSION     DAZGBC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.200"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(80..223)
                     /gene="ypaB"
                     /locus_tag="ACHK1J_004424"
     CDS             complement(80..223)
                     /gene="ypaB"
                     /locus_tag="ACHK1J_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO7913608.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            351..2636
                     /gene="nrdA"
                     /locus_tag="ACHK1J_004425"
     CDS             351..2636
                     /gene="nrdA"
                     /locus_tag="ACHK1J_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7913609.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2870..4000
                     /gene="nrdB"
                     /locus_tag="ACHK1J_004426"
     CDS             2870..4000
                     /gene="nrdB"
                     /locus_tag="ACHK1J_004426"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7913610.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4000..4254
                     /gene="yfaE"
                     /locus_tag="ACHK1J_004427"
     CDS             4000..4254
                     /gene="yfaE"
                     /locus_tag="ACHK1J_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO7913611.1"
                     /translation="MARVILRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4308..4958)
                     /gene="inaA"
                     /locus_tag="ACHK1J_004428"
     CDS             complement(4308..4958)
                     /gene="inaA"
                     /locus_tag="ACHK1J_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO7913612.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIGGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5039..6229)
                     /locus_tag="ACHK1J_004429"
     CDS             complement(5039..6229)
                     /locus_tag="ACHK1J_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7913613.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6381..7259
                     /locus_tag="ACHK1J_004430"
     CDS             6381..7259
                     /locus_tag="ACHK1J_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7913614.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT         2026 a         1968 c         1807 g         1811 t
ORIGIN      
        1 tgcttagatc aatttttgca atcattagca aaaagattaa taaaccatct atatcaattt
       61 atctaaccta ttatgccgtt caagaaatcg ccgaacagtt atttttaaca aatttttctc
      121 ttcccattga ctttcccgga caccttgtct gacctaaggt gcgcgaaagc cactttttcc
      181 ttcctgagtt atccacaaag ttatgcactt gcaagagggc cattttcaca ctatcttgca
      241 gtgaatccca aacatacccc ctatatatag tgttctaagc agcttcccgt actacaggta
      301 gtctgcatga aactattgcg gaaagaattc caaaaacagg tacgacatac atgaatcaga
      361 atctgctggt gacaaagcgc gacggtagca cagagcgcat caatctcgac aaaatccatc
      421 gcgttctgga ttgggcggca gaaggactgc ataacgtttc gatttcccag gtcgagctgc
      481 gctcccacat tcagttttat gacggtatca agacctctga catccacgaa accattatca
      541 aggctgccgc agacctgatc tcccgtgatg cgccggatta tcagtatctc gccgcgcgcc
      601 tggcgatctt ccacctgcgt aaaaaagcct acggccagtt tgagccacct gcgctgtatg
      661 accatgtggt gaaaatggtc gagatgggca aatacgataa tcatctgctg gaagactaca
      721 cggaagaaga gttcaagcag atggacacgt ttatcgatca cgaccgtgat atgaccttct
      781 cttatgctgc cgttaagcag ctggaaggca aatatctggt acagaaccgt gtgaccggcg
      841 aaatctacga aagcgcccag ttcctttata ttctggttgc tgcgtgcctg ttctcgaact
      901 acccgcgtga aacgcgcctg caatacgtga agcgttttta cgacgcggtt tccacattta
      961 aaatttcgct gccgacgcca atcatgtccg gcgtgcgtac cccgactcgt cagttcagct
     1021 cctgcgtact gatcgagtgc ggcgacagcc tggattccat caacgccacc tccagcgcga
     1081 ttgtgaaata tgtttcccag cgtgccggga tcggcatcaa cgccgggcgt attcgtgcgc
     1141 tgggtagccc gattcgcggt ggcgaagcgt tccataccgg ctgcattccg ttctacaaac
     1201 atttccagac tgcggtgaaa tcctgctctc agggcggcgt gcgcggaggt gcggcaacgc
     1261 tgttctaccc gatgtggcat ctggaagtgg aaagcctgct ggtgttgaaa aacaaccgtg
     1321 gtgtggaagg caaccgcgtg cgccatatgg actacggggt acagatcaac aaactgatgt
     1381 atacccgcct gctgaaaggt gaagatatca ccctgttcag cccgtccgac gtaccggggc
     1441 tgtacgacgc attcttcgcc gatcaggaag agtttgaacg cctgtatacc aaatatgaga
     1501 aagacgacag catccgcaag cagcgtgtga aagccgttga gctgttctcg ctgatgatgc
     1561 aggaacgtgc atctaccggt cgtatctata ttcagaacgt tgaccactgc aatacccata
     1621 gcccgtttga tccggccatc gcgccagtgc gtcagtctaa cctgtgcctg gagatagccc
     1681 tgccgaccaa accgctgaac gacgtcaacg acgagaacgg tgaaatcgcg ctgtgtacgc
     1741 tgtctgcttt caacctgggc gcaattaata gcctggatga actggaagag ctggcaattc
     1801 tggcggttcg tgcacttgac gcgctgctgg attatcagga ttacccgatc ccggccgcca
     1861 aacgtggagc gatgggtcgt cgtacgctgg gtattggtgt gatcaacttc gcttactacc
     1921 tggcgaagca tggtaaacgc tactccgacg gcagcgccaa caacctgacg cataaaacct
     1981 tcgaagcgat tcagtattac ctgctgaaag cgtctaatga gctggcgaaa gagcaaggcg
     2041 cgtgcccgtg gtttaacgaa accacttacg cgaaagggat cctgccgatc gatacctata
     2101 agaaagatct ggatgccatc gctaatgaac cactgcatta cgactgggaa gcactgcgtg
     2161 aatcgatcaa aacgcacggt ctgcgtaact ccacgctttc tgccctgatg ccgtccgaga
     2221 cttcttcaca gatctctaac gccactaacg gtattgaacc gccgcgcggt tacgtcagca
     2281 tcaaagcgtc gaaagacggt attctgcgcc aggtagtgcc ggactacgag catctgcacg
     2341 acgcctatga gctgctgtgg gaaatgccgg gtaacgatgg ctacctgcaa ctggtgggta
     2401 tcatgcagaa atttatcgat cagtcgatct ctgctaacac caactacgat ccgtcacgct
     2461 tcccgtcagg aaaagtgccg atgcagcaat tgctgaaaga cctgctcacc gcctacaaat
     2521 tcggggtcaa aacgctgtat tatcagaaca cccgtgacgg cgctgaagac gcgcaggacg
     2581 atctggtgcc gtcaatccag gacgatggct gcgaaagcgg cgcatgtaag atctgatatt
     2641 gagatgccgg atgcggcgta aacgccttat ccggcctacg gctcggtttg taggcctgat
     2701 aagacgcgcc agcgtcgcat caggctccgg gtgccggatg cagcgtgaac gccttatccg
     2761 gcctacggct cggatttgta ggcctgataa gacgcgccag cgtcgcatca ggcacaggat
     2821 gcggcgtaaa atgccttatc cggcattaaa ctcccaacag gacacactca tggcatatac
     2881 caccttttca cagacgaaaa atgatcagct caaagaaccg atgttctttg gtcagccggt
     2941 caacgtggct cgctacgatc agcaaaaata tgacatcttc gaaaagctga tcgaaaagca
     3001 gctctctttc ttctggcgtc cggaagaagt tgacgtctcc cgcgaccgta tcgattacca
     3061 ggcgctgcct gaacacgaaa aacacatctt tatcagcaac ctgaaatatc agacgctgct
     3121 ggactccatt cagggtcgta gcccgaacgt ggcgctattg ccgcttattt ctattccgga
     3181 actggaaacc tgggtcgaaa cctgggcgtt ctcagaaacg attcattccc gttcctatac
     3241 tcatatcatt cgtaatatcg ttaacgatcc gtctgttgtg tttgacgata tcgtcaccaa
     3301 cgagcagatc cagaaacgtg cggaagggat ctccagctat tacgatgagc tgatcgaaat
     3361 gaccagctac tggcacctgc tgggcgaagg cacccacacc gttaacggta aaaccgtaac
     3421 cgttagcctg cgcgagttga agaaaaaact gtatctctgc ctgatgagcg ttaacgcgct
     3481 ggaagcgatt cgtttctacg tcagctttgc ttgctctttc gcattcgctg aacgtgagct
     3541 gatggaaggt aacgccaaaa ttattcgcct gattgcccgc gacgaagccc tgcacctgac
     3601 cggcacccag catatgctga atctgctgcg cagcggcgcg gatgatccgg agatggcgga
     3661 aattgccgaa gaatgtaagc aggagtgcta tgacctgttt gtccaggcgg cacaacagga
     3721 gaaagactgg gcggattatc tgttccgtga cggttcgatg attggtctga acaaagacat
     3781 tctctgccag tacgtggagt acatcaccaa tatccgtatg caggcggttg gtttggatct
     3841 gccgttccag acgcgctcca acccgatccc gtggatcaac acctggctgg tgtctgataa
     3901 cgtgcaggtt gctccacagg aagtggaggt gagttcttat ctggtcgggc agattgactc
     3961 ggaagtggac accgacgatt tgagtaactt ccagctctga tggcccgcgt tattctgcgc
     4021 atcactggca cacaactgct gtgccaggat gaacaccctt cccttctggc ggcgctggaa
     4081 tcccacaatg tggcggttga gtaccagtgc cgcgaaggtt actgcggctc ctgtcgcacg
     4141 cgccttgttg caggccaggt ggactggatt gccgaaccgt tagcctttat tcagccgggg
     4201 gaaattttgc cctgttgttg ccgggcaaaa ggcgatattg aaatcgagat gtgaattggt
     4261 tgtagtgcca gatacaacgc ttatgcgtat tatctggcct acaacgatta catggcgtag
     4321 taatacgctt ttacctgctc ccagtccgct ttggggatcg gctccagata tttttccagc
     4381 tggcggaagt catgattaat cgctttatcg cgacgcaagc ggcgacgact tttctccaga
     4441 tcaagaaaac cagcttctgc cttaccttct gttttcacat agatatggcg aacataacaa
     4501 cagccatgct gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac
     4561 atggcttgcc gtacttcgtc agaatagggc gttactgcat gctgggcata ccagtcagca
     4621 atgctgatga accccgccat atcttcagtc accagcaacg cgcgccattc acccccaatt
     4681 ttcaccgctt caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta
     4741 attaccgcaa cctcacggac aatcgttggt cggccgaaag gataacgtac ggaatgaaac
     4801 agatgatgcg tcatgcgctt tacatatagc tttttgccgt tgcgctcgac gcattgcacc
     4861 ccgctcatac cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac
     4921 cagtgattaa attcgtcgtg ctttgctgaa actgccatac cctatcgcct gtcatttttt
     4981 attaacgaca atgactatag gtggttacct gcgaaaaatc ttaatgaaac gtgtcgtatt
     5041 aatgaatttc ttttgccact actagcgcga caccgctttt acccttcatt tgcagcgcat
     5101 agactccacc agccactacc actaatagtg cgcccagcag ataaatcatt cggtagccag
     5161 tatgtgcaac catcatcccc atcacacagg ctcccagcgc caggcctgca tccatcgcat
     5221 tgaagaagag ggagtttgcg acaccgattt tatgcggttc cactgaactg ataatctgcg
     5281 tctgaaaaac tggcgtcacg gaaccatacc cgataccaat caacgcgcca gaaacgacca
     5341 tcattacgct gccctgggtg tagccgagaa ccaccagccc aaccgtaaag aacagcaggc
     5401 aggggtaaac cacatatttc gggccttttt tgtcgcaaac attgccggtg aaggtacggc
     5461 agatcatcag gaagatggcg tagcagagca ggaaattact ggccgccgac atgagattaa
     5521 gttcgcgggc gtaaagcgcc agaaaggcag aaaccccggc atacgaaaag gtcataaaga
     5581 atgtaaccat ggcaaacggc aatgccgcgc gatcgaacat ggcggcgaag cccatctttg
     5641 gctttgaacc atcggcatgg ctgataaccg gcggcacggt cataatcagc gacaggataa
     5701 tccccaccgc cgcaacgcca gtacataacc agaaagccat attaaaggcc tcccagcgcg
     5761 ccatattcag gccaatccac ggcccgacca ccattgccag ccccattgcc agagagaaaa
     5821 aactgatgcc ctccccacgt cgggaggccg ggatcagccg cgcggaaatc gttcccttta
     5881 ccgttgtgat aaccccgaag gtaataccat gaagcaccct aataaaaagc agtgattcta
     5941 ttgagtggca aaaaggatac agcgcagtga ccaccaaaaa ggccagagaa gagagcacca
     6001 gaatagtttt attcgaatat ttacccaccc attgtccggc aaagggtcga ataacaatcg
     6061 ccgcaattaa aaataatgtc accaacaaac ctgctttatc aggagaggca tgcaagcggt
     6121 cagagatata aattggcaac aatgttagta aaacataaaa gacgaagaaa ataataaagc
     6181 tgatgatggt tattgcccaa aaatccttcg tccataactt ctctttcata ccctaccgcc
     6241 tgtcaggatg acgcacgcag acaacactcc cgttactgcg gttgcgtcaa attgtgatgt
     6301 actttgcaga acagaagtat aaatggcggc atgctattag agaaattaat cgttttaata
     6361 ttattgatta gcaaactcta atgactctta cccaaataaa ttctttactc gccgtgctgg
     6421 attacggtgg ctttactgaa gccagcaagc gattgttcat gacgcagtcg gcggtaagtc
     6481 aggcaattgc tgccctcgaa caagaacttg gtgtgaatat actgttacgc gatcgccgta
     6541 aagctataca actgaccaca gcaggtcacc gcattgtgca acatctgcgc gccattaatc
     6601 gggaagttaa cgcagtaaaa gagatagccg agcaggaaaa gcagaaccct caaagaacgc
     6661 tgagacttgg ttgtttcccc agcgtctgcg cctgtatctt accggttgtg attcgctact
     6721 ttgaaattca tcatccgaat ataaaaatta ttccttttga ggaaaatagc acagcgatta
     6781 ttgattcgct tcagaatgaa tccattgatg ccggttttgt tcattttccg gtgagcggaa
     6841 tgtactccgt gcccatttat caggataaat tcaccgtagt gttaccgccc aaccatgctc
     6901 tggcaaaaaa cagcactatt acagtggaag aattaatggg tgaaccatta atcattagta
     6961 aaggacgcta tgaattaagc attatggcac tatttaaaga aaagaatatt accccacaga
     7021 taaaatatga atttaatcac ccggatacag cgatcagttt tattcgacag ggattaggca
     7081 tcgccttatt accagagtta acgctgaaaa ccattgcaga tgaattgtgt tccgttcctc
     7141 tcgaaccaac cttctatcga cagatttcgt tgctggctaa agaaaagccg gtagaaggca
     7201 gtccactgtt tttactacaa atgtgtacgg aacaactagt ggtgagtgga aaaatttgat
     7261 gtaagacatg agattgatga caaatgcaag actgcctgat tcgccacact tagttgtact
     7321 atagccttta ccaggcgtaa taattcgttt tcacctgatt ttggcccact ttgtgaattg
     7381 gcgatatatt tatccagttg ggagaagtga caatgactcc aggtagaaac cggtatgaag
     7441 cgcccgactc actgtcaaat ccgattgttg agagtaatga cactccggcg aacagtacac
     7501 agtttactct ggagccattc tcaatcttca agtgggatac ctaataactt gtcagggaag
     7561 ttatcaatat ctgtttgtta cttattaccg tatttattaa caataatgat ac
//
LOCUS       DAZGBC010000200         7413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGBC010000200 DAZGBC010000000
VERSION     DAZGBC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.201"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..101
                     /locus_tag="ACHK1J_004431"
     CDS             <1..101
                     /locus_tag="ACHK1J_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016808261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO7913615.1"
                     /translation="TIRDGIDHYLAPSRAQPRSSAAVTGDYRRERP"
     gene            101..343
                     /locus_tag="ACHK1J_004432"
     CDS             101..343
                     /locus_tag="ACHK1J_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO7913616.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            420..734
                     /locus_tag="ACHK1J_004433"
     CDS             420..734
                     /locus_tag="ACHK1J_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO7913617.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            747..1109
                     /locus_tag="ACHK1J_004434"
     CDS             747..1109
                     /locus_tag="ACHK1J_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO7913618.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGVIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            1106..1768
                     /locus_tag="ACHK1J_004435"
     CDS             1106..1768
                     /locus_tag="ACHK1J_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO7913619.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            1768..2676
                     /locus_tag="ACHK1J_004436"
     CDS             1768..2676
                     /locus_tag="ACHK1J_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO7913620.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTVASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNAHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            2666..4153
                     /locus_tag="ACHK1J_004437"
     CDS             2666..4153
                     /locus_tag="ACHK1J_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO7913621.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEHQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            4189..4581
                     /locus_tag="ACHK1J_004438"
     CDS             4189..4581
                     /locus_tag="ACHK1J_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO7913622.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            4719..>7413
                     /locus_tag="ACHK1J_004439"
     CDS             4719..>7413
                     /locus_tag="ACHK1J_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO7913623.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLVGRE"
BASE COUNT         1610 a         1775 c         2464 g         1564 t
ORIGIN      
        1 agaccattcg tgacggtatt gaccattatc tggccccttc ccgggcgcaa ccccggtctt
       61 ccgcagccgt gaccggtgac taccggcggg agcgtccctg atggcgatga cggccctgca
      121 gaatacggcc ttccgggctg cttcaggtga agtggatctc agtgtgctgc atctcatcac
      181 gctgggattt ctgctggcag tcgtgttcct ctgggcagcg tggagtctga cggatgcctg
      241 ggcaggctgg cgtagcggtg atgtccggga tggtgtgttc ggctggttta tgttccgact
      301 cgtcctgctg ctgatctgca gtatctggat gtttgcgggt tgatttcagt gtcttgttta
      361 agtttcagga gaaaataatg cataaaaaaa tgcgcatctg tcgccaggcc tggtatgcca
      421 tggcggtgtt tctgtgtacc gggatcacac ctgctctggc tgatttgcct cagatagagc
      481 cgccaacatc cggtggaggt ggtggacttt tcggacagat caaaggctat ctccaggatg
      541 gtgttgtgct gtttggtctt gtgctcagtg cagttgcttt tgtgaaggtg gcggaagcgg
      601 cgctttccac cttctcagag gttcgtgacg ggaaggccgg atgggggcag tttggtgcca
      661 tcgtggtggt tggtgttgtt ctgctggtcg ccattatctg gctgctgggc aagtcggcct
      721 ccatcattct gtaaggagac atccccatgc agaccattcg ttttctgccc gatcgtctga
      781 acgtggagcc tgcggttttt cgtggtttta ccacaccgga actggggctg gcggccctga
      841 caggcgctgt ggcaggggtg atctggccgt taccgctgat tccgctactt ggctgggtga
      901 ttatcccgac agggatgttg ctgactcccc tgctgctggt ctggttcggt ggcgtctgga
      961 tcagccggct gaagcgggga aaacctgaca actggctctg gcagcatctg gcgaagaaaa
     1021 gcagtgcatg gggtggtggc cggaaaacat ttatccggga ggcgcatggc tggtccctgc
     1081 gccgtacccg tagcaggggg aaaaaatgag tcgttttcgt catgctctgc gggatcgcga
     1141 ccagcatatc ctgacactgc gtattttcag cggtgtgctg gccggaattc tgatgctggt
     1201 ctgtatcggg tggatgacgg caccggacag actgacaatt cacaatccgc cggatctgcg
     1261 ttccggcagt acccgtccct ggtgggaagt cccgccgtca acggtttatt cctttgcctt
     1321 ttatatcttt cagcaactga atgcctggcc gaagaacgga gaggtggact acccggcgaa
     1381 aattcatgcg ctctccgcct acctgacgcc atcctgtcgg gagattctgg agagtgatgc
     1441 ccgccgtcgc agtgatgcgg gggagctgct ggagcgtgtg cgcgtggtgt atgaaattcc
     1501 ggaacatggc tacgggggca ggtacaacaa aaatccggtg acggtggaag ggcgggataa
     1561 ctggcaggta cagctggatc tggtggcaga tgagtatttt catgctgagc cggtaaaacg
     1621 ggcacttgtc cgttatccga taaaggtggt gcgcatggag ggggatcctg aacgtaatcc
     1681 gttcgggctg gcgctggact gctacagcag tacaccacag cggctggaag cagcctcccc
     1741 ggcagccggg aagggggata atcagtgatg aacggagctg aacgttgttt ataccgtagt
     1801 attgtgatgg tcctgtcact gctggtttta ccactgagcg cacgggctga tgagctgatg
     1861 aaatgggagc gcattccctt acccgttacc ctgatggcag gacaggagcg ggttattttt
     1921 gcagataaaa atgtgcgggt gggcttcccg ccagcactga acggaaagtt gagggtacag
     1981 agtaccggtg gggcagtcta tctgaaagca gagagtgttt ttccgcagac gcgtctgcaa
     2041 ctgcaggatg tggagagcgg agaaattatt ctgctggatg tgacagcagc agaaaaaggg
     2101 cccacagagc ccgtacggat tgtgtatgag ggaagcgtca gtacagtcag tcaggctggc
     2161 acagtggcgt ctgcacgctc tgcagaacac aaaaaggtgg tacccggcgg ggatgacggc
     2221 tcgcaggccc tccgtcagcc ggtgaagtat gcagagcctt tacccgttct gttgacccgc
     2281 tatgcggcac agtcgctgta cgcaccgtta cggacgctgg aagcggtacc gggtatccgg
     2341 ccggttaatg cgcatctgcc cgggaaaatc accacactct atccgtcaga gccactgaca
     2401 gtcacgccac tggcagcctg ggctgcgggt gggcgtgcag ttgttgcact gaaactgacg
     2461 agtacggtaa gccgcaaaat tatccttgat ccgcgggcac ttcagggaaa attcattgcc
     2521 gcgactttcc agcaccgctg gctgggtgca gcaggtaccc ctgaggatac caccacgctc
     2581 tatgtggtga cggcagaacg gccggaacag gcgtttatcg cggagcctgc ctcccgtcgc
     2641 attccttcac ggggggaggt gaagcatgca gattaaatcc aatacgatcg tgaagtatgt
     2701 ggtgccctgt gtggtggtgg cggggatcct gacgggatta aaaagctgtg gaggagatac
     2761 cggggagccc tcgtctgcgg aaagctcagg aacgctggcc ggactgtcac cggatgaact
     2821 gaaggccctg ggcgtggaag gagacacacc tgaagatacc ctgcgcacac tgattgggcg
     2881 tatgaatcag gtgagggagc ggcaactggt actggacaaa cagaatgaaa agttgctgaa
     2941 agaaaatgaa atgctgcgca agcggggcac ggatgtttcc ggtcaggtca gtgaggcagt
     3001 ggcaggattg cagaaaaatt atgagcagtc gcagcgccag ttccggaatg aacagaacgc
     3061 gttaatgaca caggttcagt cactgacaga gcaactgaag cgtcagcagg agagtgctga
     3121 cgccggcgcc ggaggacagg agagcgatat tccagcaggt ctgggccttg acggactggg
     3181 gagcgtgaca ggatcggcag cctcaccggg aacggatggt ctgatgtggg tgacaccggc
     3241 cgatcaggtg caggatgaag cgtcgtcggt gggggataag agcggaccgc gttttccagt
     3301 ctcttttctg gatgacaatc cactgacccg tcagaaagcg gcttatgaac agcaggtgaa
     3361 gggatacagc aatgaacatc agaaggcgag cacccgggca gtgtataccc tgccggagaa
     3421 ctcgacgctg gcaggcagcc gggcgatgac agcgcttctg ggacgggttc cggtgaacgg
     3481 aaccgtgacg gatccctatc cgttcaagat cctggtgggt aaggataacc tgacggcaaa
     3541 cggaatagag ctgccggatg tggaaggtgc agtggtgtcc ggtactgcga gtggtgactg
     3601 gacgctctcc tgtgtccgtg ggcaggtgaa cagcatcacg tttgtttttg ccgacgggac
     3661 agtgcgtacg ctgccgtcat cagacgtgaa cggcagcagt aacgcacagg caaaaaataa
     3721 cagcagtaca ggaggcattg gctggatttc ggatgatgac gggatcccgt gtatcagcgg
     3781 aacccgtaaa tccaatgcct ccacgtatct gccgacaata tttgccctgt ctgcagccgg
     3841 cgctgcgggt gatgcactga gtgaggggca gtacacctca cagaataacg tcaatggcat
     3901 cacatcaaca ctgaccggca gtgccggaga ggcggcactt ggaaaagccc tgtccggcgg
     3961 gatgaatgaa accgcggaat gggtgaaaca gcgatacggg cagacgtttg atgccattta
     4021 tgttcccccg gggcagacgc tggcggtgca tatcacgcgt caactggcca ttgattatga
     4081 agatcagggg cgccgggtac gttacgactt cactctgccg ggggaagtcc acggtaccgg
     4141 tggtctggac tgatgtaagg agatcccgtg aaacgacagt tatctgtaat gaccgtcatg
     4201 gtggcggtat ttattctgcc cggctgctcg acatcgaaag aggagcttct gcctccgggt
     4261 gaacagagta tgctggagat ctggcagggg aaaggaagcg gaggacattc gcggcacatg
     4321 acagcggcca gggacaccct gcggcgtccg ctgacaatgc cggagcagca ggcagatgcg
     4381 cacggagcca taagctacag ccggacacag gaaagtgaga tcagtcagca gtttccgcgg
     4441 ctgcccaatc ctgacatggt gatgtatgtt tttccgcatc tggcttccgg tacgtcaccg
     4501 gttccgggat acagcacagt atttcctttt tacagccgga cgcagtatgc catgccgggt
     4561 gaacgaacgg aggcgttgtg atgcgatttt ccctgtttac agacacaaaa aaacgggttt
     4621 cttccccggt gtcgcagcat gacggagaag cggtatctcc ggatatgaaa gaaagagtgg
     4681 cccctcaggt acgaaagcca ctgtcaaggc cggggaagat gacccgacag gatgagacga
     4741 aggtttacca tgcctgtccg tcggtgattg attttctgcc atgggtggaa tatctggacg
     4801 aagagcagtg cctgctgctg gatgatgggg tgtcggttgg tgccgtgtat gaggtgagtc
     4861 cggccgctac ggaagggcgg accgcagagc gtctggagca aatccgggat acggtggagg
     4921 atgcgctgca ggacagtttt gatgaatatg actcacatcc ctgggtggtg cagttcttct
     4981 gtcaggatga aaacgacgtg gacacgtatg ttgaccagct cagggggtac gtgaaacctc
     5041 atgcagaagg ctcctcattt acggaggcct ggctgaggga gatggagcgc catctgaagg
     5101 ggattgcccg accggaaggt ctgttcaggg atacgctggt cacaggtcag ccatggcgtg
     5161 gtcagcagcg gcgcacgagg atggtgattt accggtggat cgggaaaaac aaccacgatc
     5221 cgatgccgcc ggtggcaatg ctgaaccagg tctgttcgcg ggtcgtgggg gcgctgggtg
     5281 gcgcaggcgt ccgctgtaca cggatgaacg ggcagcaggt gcatggctgg ctgctgaggc
     5341 tgttcaatcc ccggccggaa tgggtggaca gggatattct gtaccgtatg gcatcccgtg
     5401 cagagccgca ggagacaccg gagggaatga tgccggtgat gaccgatttt gcagaaagcc
     5461 tctggttcac cccaccggta tctgatccgg aaaacggcgt gtggtggctg gacgggcttc
     5521 cgcatgccgc tgttgtggtg gagaaactgc gcacaccacc ggagccggga accatcacag
     5581 gggaacaggc ccggggcgaa aagacagtga atgccctgat ggacaccttt ccggagggaa
     5641 cggtgctgtg tatgacaatt gtggtccagc cacaggacac actggaagag cgcttcacga
     5701 ggttgtcgaa aaatgcggtc ggggagaaca gtgaatccct gcgtgcccgg gaggatgtgg
     5761 cgaccgtgaa gaaatatctg ggaaaccggc acaagctcta ccgtgccggg atcacgtttc
     5821 tgctgcgggc agaggatatg gagagtctga agcgcagacg ggttgaactg acgacggtgc
     5881 tgctgggcgc gggcttacag ccagtgcgcc cggagtttga tgtcggaccg ctgaacagct
     5941 ggctgcgcgc gctgcccatg tgttttgatc ctgatacgga taaaaaacag tggtacacgc
     6001 gtctgatgtg ggtacagcat ctggcagggc tcctgccggt gacgggaagg gaaaccggta
     6061 cggggcatcc cggattcagt ttctttaacc gtggcgggga tgtgctgacc tttgatccgc
     6121 tgaacaagct ggaccggacc cagaatgcac atctgctgct tttcgggcct accggagccg
     6181 gtaaatcggc cacgctgtgt ggttcgttgt cgcagataat ggctgttcat cgtccccgtc
     6241 tgtttatcgc tgaggccggg aactcgtttg gtctgctggc ggattatttt gagagtctcg
     6301 ggctttcggt taataaaatc agtgtcaaac cgggaacggg ggtgtgtctt cctccatttg
     6361 cggatgctca tcaactggtg gagcagggag agacgctgca gagtgtggat gagcattcac
     6421 tgcccgatct tgatgaagat gagggggatg aagaggaaga gaaacgcgac attctggggg
     6481 agatggaaat ctctgcccgc atgatgatca ccggagggga tccgaaagaa gaagcagcgc
     6541 tcaaacgtgc ggacagggcg atgatccgtg aggcactgct gatggccacg cacaccacgt
     6601 accgggaggg gcgtcagatg ctgccggtgg atctgcagag tgcgttgtgg gaaatttcgc
     6661 gggacactca gcgtaatgat gttcgccggg ctaaagctgc tgagatggca gagtcgctgg
     6721 ggatgttcac ccagccgggc agttttgaag cggagctgtt taaccgggaa ggaaaactgt
     6781 ggccggaagc ggatgtgacc ctgattgatc tggggcatct tgcccgtgaa ggttatgaag
     6841 cccagatggc actgaccatg gtgtcaatga ccaatatgat taacaacatt gccgagcgcg
     6901 atcagtatct gggacgggat attctgtttg tggtggatga ggcgcatatc gtgacggtaa
     6961 acccgctttt gtccccgtat atgaccaaag tggtgaaaat gtggcgcaag ctgggggcct
     7021 ggctgtggct ggccacgcag aacctgaagg attatccgga tgtctcggag aagatgctga
     7081 atatggcaga gtggtgggtg tgcctgacca tgccgccgga ggaagtgaac aatattgccc
     7141 gttttaaatc cctgacggag gagcagaagg cggtactgct gtcagccagt aaactgtccg
     7201 gctgctatac ggagggggtg gtactggcga agcgcacaga agcgttgttc cgggttgtgc
     7261 caccgagtct gtatctggct ctggggatga cggagaaaga agagaaggct gaacgacgcg
     7321 ccctgatgaa ggtgcaccag tgcagcgaac tggaagcagc gtttcatgtg gcacgaaagc
     7381 tggacgggct acgtggtctg gttggcaggg agt
//
LOCUS       DAZGBC010000201         7378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGBC010000201 DAZGBC010000000
VERSION     DAZGBC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.202"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..233
                     /locus_tag="ACHK1J_004440"
                     /pseudo
     CDS             1..233
                     /locus_tag="ACHK1J_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            complement(263..853)
                     /locus_tag="ACHK1J_004441"
     CDS             complement(263..853)
                     /locus_tag="ACHK1J_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913624.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     KVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(853..1110)
                     /locus_tag="ACHK1J_004442"
     CDS             complement(853..1110)
                     /locus_tag="ACHK1J_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913625.1"
                     /translation="MDEERMFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            1671..1889
                     /gene="ccdA"
                     /locus_tag="ACHK1J_004443"
     CDS             1671..1889
                     /gene="ccdA"
                     /locus_tag="ACHK1J_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO7913626.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            1891..2196
                     /gene="ccdB"
                     /locus_tag="ACHK1J_004444"
     CDS             1891..2196
                     /gene="ccdB"
                     /locus_tag="ACHK1J_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO7913627.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            2197..3003
                     /locus_tag="ACHK1J_004445"
     CDS             2197..3003
                     /locus_tag="ACHK1J_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7913628.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVVAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            3182..3826
                     /locus_tag="ACHK1J_004446"
     CDS             3182..3826
                     /locus_tag="ACHK1J_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO7913629.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            3913..4221
                     /locus_tag="ACHK1J_004447"
     CDS             3913..4221
                     /locus_tag="ACHK1J_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO7913630.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATVPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            4633..5613
                     /locus_tag="ACHK1J_004448"
     CDS             4633..5613
                     /locus_tag="ACHK1J_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO7913631.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGGDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            5606..6022
                     /locus_tag="ACHK1J_004449"
     CDS             5606..6022
                     /locus_tag="ACHK1J_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7913632.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(6024..7298)
                     /gene="umuC"
                     /locus_tag="ACHK1J_004450"
     CDS             complement(6024..7298)
                     /gene="umuC"
                     /locus_tag="ACHK1J_004450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFO7913633.1"
                     /translation="MFALADINSFYTSCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQIRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLREVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGTGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPHKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(7298..>7378)
                     /locus_tag="ACHK1J_004451"
     CDS             complement(7298..>7378)
                     /locus_tag="ACHK1J_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO7913634.1"
                     /translation="FRTLYPENICIFGVVTHVIHRTRELR"
BASE COUNT         1809 a         1738 c         1827 g         2004 t
ORIGIN      
        1 agaaaaaagc ccgggccatt gccttgatgg acagcaaaaa gatgtggcgg agtaattatg
       61 cccctcctct tctgcgtatt ctctggcggc tgggtatccg gcttctgcca ttacccttta
      121 tgccgttctg gcaggtcacg ctgttgatgg ggagcttata gggcattttc tggggatgtg
      181 caatgtggtt tatctactgg tggcgcagga aagttaaccg gctgccacca taggatgatt
      241 tgtaaaccgt gcaggcatgt ttttacgggg tgatgctttc attaacctgc acacgaacct
      301 ccattcctgc atcgtgacag gcctgcagcc actgcgccac ttccagcgga tcgccctccc
      361 ggcgtaccac cttgccttct ttattccata actgcagaca ggtgctgccg tcgagacgca
      421 ccacaaaatc cccacggcag gcctgatacg ttatgccgtt cagctccacc gccagatcag
      481 tggcagaagt gccgtctgcg ggcagacctg ctgccgggat cagcggcagt acgacgactt
      541 ctgccgcacg ctgttctgcc cggaggcggt cctgttctgc cagcagcaga ggctgtgcca
      601 cggcccggcc ggcgtccgtc agctccactg ccagctgcag gttcggagcg cgcagggtac
      661 gcagccagcc ggcatcctcc agacgacggc aggaggcgcg caggtttggc ccgtagaccg
      721 gggcttcccc gcttcgctcc agcacccgtt ctatgtcccg cgtcagcacg gggcccggcc
      781 gtttttcatc aagcgcagcc agcacaatca gcactctccg ctgcagtggc gacggtcggc
      841 ggctctgggt cattacctgt catcctccgg ccagatgagg tttctgaggc gctgaaagtc
      901 ggcgtcaatg agctcctgtc tctttatggc attcattgag gcatagccat ttatggccag
      961 ttcatgccag aagatcagca cggctccggc tttggctgac tcgcagagat aaattgcgcc
     1021 ctggctgtcc agattgcatt cccggatgcg tttttccgca aaccgggtca gttgttcata
     1081 gctgagtgaa aacatccttt cttcatccat cctgcacact ccatctgata atatcctgtt
     1141 aagaaataaa tcatatcttg aatcatttat ttctgcacct gtaaacatca tatcagatat
     1201 gatttcatgt gtattgcttg attatcatat tagttgctgg ttttccgccg cgcagatccg
     1261 gtttccggtg gtcacggatc ttcctctccc ccacacaacg ccacctcctg taagtacaac
     1321 atgtagtgct tcattcggct gctcatgaca ctatatgttg tgttatttcc ctgacctgtg
     1381 atgcgccacg caggggcgga aaacagcgat atgatgattt cctcagcgtg gtacacttcc
     1441 ggaaacccgt caataacaga aaaatcggat ctgccggaaa tggtccttcg ataattacgc
     1501 tttgttttta taagtctgat tttaattttt attggttaac ataatgcaag gtaaaataaa
     1561 taaggcttac taaaagcaag ataacagtat gtgtattaac gcgctgattt ttgcggcata
     1621 agaatatata ctgatatgca taccagaagt atgttaaaaa gaggtgtgct atgaagcagc
     1681 gtattacagt gacagttgac agcgacagct atcagctgct caaggcgtat gacgtgaata
     1741 tctccggtct ggtcagtacg accatgcaga acgaagcccg gcgactgcgt gctgaacgct
     1801 ggcaggctga aaatcaggaa gggatggcag aggtcgccag gtttattgaa atgaatggct
     1861 cttttgccga tgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga
     1921 gagagccgtt atcgtctgtt tgtggatgtc cagagtgaca ttattgacac gcccgggcga
     1981 cggatggtga tccccctggc cagtgcccgc ctgctgtcag ataaagtctc ccgtgaactt
     2041 tacccggtgg tgcatgtcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     2101 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccggga aaatgacatc
     2161 aaaaatgcca ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc
     2221 cagtctgcag ccatggtacc ggcagtgtat tctgccggac agtctccaca acttcctgtt
     2281 gtcattgact atccggcagc tctggcactt cgccagatgt cgatggttca tgatgaactg
     2341 ccgaaatatc tgctggctcc ggaagtgagt gccctgctcc attacgtccc ggatctgcgc
     2401 cgcaagatgc tgttggccac actgtggaac accggtgcgc gcattaatga agcactggcg
     2461 ctgacgcggg gggatttttc gctcgcgcct ccgtatccgt ttgtgcagct tgcgaccctg
     2521 aaacaacgga ccgaaaaagc tgccaggacg gcggggagaa tgcccgccgg tcagcagact
     2581 caccggctgg ttccgctctc tgactcctgg tacgtcagcc agctgcagac gatggtggca
     2641 acactgaaaa tacccatgga gcggcgtaac cgtcgcacag gaaggacaga gaaagcgcgg
     2701 atctgggaag tgacggacag aacggtcagg acctggattg gggaagcggt tgtcgccgct
     2761 gccgctgacg gtgtgacgtt ctctgtcccg gtcacgccac atacgttccg ccattcctat
     2821 gcgatgcaca tgctgtatgc cggtataccg ctgaaggtcc tgcagagcct gatggggcat
     2881 aagtccatca gctcaacgga ggtctacacg aaggtgtttg cgctggatgt ggctgcacgg
     2941 caccgggtgc agttttcgat gcctgagtcc gatgcggtgt ctatgctcaa aagaattcct
     3001 taagctctga cttttttaaa tccccttagg atatctcatg gatatattat cggtatttca
     3061 tattctgttg acatgcagga ccggtataga gtaaaaaacc tctattgagt acctcttgga
     3121 tatccgaaag gtgtcttttg ggttctttat gggtattaca tgtggattct ggagatacac
     3181 tatgcctgtt attgctaata cacatcccaa aggtggtgtg ggtaagacaa cttcatccgt
     3241 aaacattgtt ggtgaaatga agtctgatac agttgatctg gatactcata ccgggctttc
     3301 tatcattctg gggctgagac ctgaaggaaa agaaatttcc gtgaaagtac ccaaaacagt
     3361 ggatgagtta atcgaaatta tgactcccta caagaacagc gataagacgt tacttattga
     3421 ctgtggggga tttgactcag atcttacccg tactgcgatt gcctttgcag actgcgttat
     3481 tgtcccttca aaagactccc tgactgagcg tattggtttg atgcattttg atggagtact
     3541 ggacgaaatc agctctatca tggggaccga tataactgct cacctgtatc tctgtaaagt
     3601 taatcctaac aagaagaaat tccctaagct tgatgcaatc ctgccatcgt tcaaacatct
     3661 gaagctgatg aagagccgca tttctgctcg tgcagaattt gaggatgtca ttgagactgg
     3721 gatgggaatc actgagtctg ttcatgggcg ctattcggca ggaggtaagg aagttattgc
     3781 ccttatcgaa gaaatcaatc atctgattga aaataacaaa cagtaaagtg tatctattgg
     3841 gtatccatat agtatccttt gggtttttaa tggatatcct ttgaatatct aacaaacatc
     3901 ctgggttatc tcatgagcaa agcgaaattt agtgcacagg ctgaaaaact gaaaaacttg
     3961 gtggattctg ccagattaca aacttcttct tctgccgtaa cagcaacagt cccgtctcag
     4021 ccaaatgaag gtgcagagga agtttcaggt cgtcccgtgg ccgccaggac gaaggctaag
     4081 aatcttaagg ctataccgct gtcgtatttt gaagctcatg cggaacttaa agcgaccagt
     4141 aaaacatctc tggattttag ctcatatatt attgaggcta taagagagaa gcttgaacgt
     4201 gatggtgcaa taggtcaata aactatcaga taccttaagg gtatctgatg tgtgtctttt
     4261 aaatgcgtta tagggattcc ttgtgtacct aaagagtccc tattagacat ctttctgtat
     4321 atggtgacat gattcgtcac tgctttcctt tcctctcagt ttcaccattt cattcccttc
     4381 ccttaacctg aaccatgatt tcaaagatgt ttttatattg catttatgtg ttgcatataa
     4441 cattcataat gtttttgtat ctatgatttc tctggtctta aggttgtatg tgcaatatac
     4501 atgcaattta ttgtgttttt atgttgcttt caccctttca gaaagaccat ctttccatta
     4561 aaattgaata tacattgcat gtatgaagtg tgtttgtttt ttctatttgt ttatgagcaa
     4621 cggagactgg ttatgaaaat ctttattgat gatggttcta ccaacattaa acttgcctgg
     4681 ttggaggatg gtgacgtgaa aacgttaatc agccctaaca gctttaaacc ggaatggtct
     4741 tttagtttac tggatgatgc tgccccggct aattacgaga tcgacggcga gaagttttca
     4801 tttgatccat taagtgctga tgctgtcgta acgactgaaa cacgctatca gtacagtgat
     4861 gtgaatgttg tggccattca gcatgcgcta cagcagaccg gcctgaaagc gcagcctgtt
     4921 gatgtcatcg tcacattgcc gatttctgaa taccttgacg ccaacaatca gaaaaataag
     4981 caaaacattg aaagaaagaa aaaaaatgtg atgcgtgaag tacgggtgca ggggggggat
     5041 gcatttgtta ttcgcagtgt ttccgttctt cctgaaagta ttcccgctgg tttcagtgtt
     5101 cttgcggggc ttgaggatga tgaatccctt ttgatcgtag atctcggtgg tacaactctt
     5161 gatgtgtcgc atgttcgtag caaaatgaca gggattacaa aaacctggtg tgatccgaat
     5221 atcggcgtgt ccctgattac atccggggtt aaggaacaga tggctgtaca tgctaataca
     5281 agagtgagct cattccaggc tgacaatatc attgtgcacc gtaatgaacc tgactacctt
     5341 tcccgtcgca tttataatgc ggaacagcgt gagtctatca ttaatgttat caatgaacgg
     5401 cagaagttgc ttattaaacg agttaatgat gtcatcagcc gtttcaccga ctatacacat
     5461 gtaatgtgtg ttggtggtgg ggctgaaatt gtggccgagg ctgtgaagaa cctgactaag
     5521 gttcctgatg agcgctttta tctgagctca tccccgcagt ttgatttagt catgggaatg
     5581 ataaaaatga aaggtggtgt gactaatgag tgatgaaaat aaatccagac gctgctcatt
     5641 tgaacttttc ccggatgaac ggacaggaga taagatagca gatgagctca ttgcaaatga
     5701 aaaactgaag gagcggggac gtttcatgcg cgcgatgtta gttactggtg cggcgtttgc
     5761 agctatagat aaacgtcttc ctttgctgat atcggagtta ctgacagaaa atacgactct
     5821 ggatgatatt aataaggtga tttccagcgt aataccaggt gctttttcgg ttgaaaagaa
     5881 attgcttgaa ttactggaaa aacaatctgg tctccatacg tctgtggatt gcagtactcc
     5941 tcttacagag cagagtctgt cgagaaatga cggtgaagac cagaccagaa gaaacgctga
     6001 aaatatgttc ggagatgact gaactaaccc agcctggcca ggggaatatc tcgccattta
     6061 gttgtataac actgactcaa catttcccgt ttcatttgcc attcaggggc ggttcctcgc
     6121 cccgcaaacc acactttccc ttttcccgac tggttcagtt catcgagtgt tttcattaac
     6181 ttttcgctgt ttttatgggg ctggatctca tcaaataatc ccggctgcgc tatacccgat
     6241 ggtgtgaaat cagccagcat gactcctgcc ttcatatagc ggtacccttc acgccagaca
     6301 tggtttaagg ctctgcatgc ggcggcaata atgtcccggc tgtcctgtgt gggcaatgga
     6361 agcttttcca cagcggcatt gctgtaacag ggttctttta ctgcaaaggg ggatgtccgt
     6421 acaaatgtcg tcacctgccg gcaatactga cgctccccac gtagtttctc tgcagcccgc
     6481 tctgcatact gaacaacagc ctggtgcatg gcatctttgt ctgtgattcg ttcaccaaaa
     6541 ctgcgactac agacaatctg ctgttttgcc ggtggtgctt cttccaggga tatgcaggac
     6601 tcgccgttga gttcgcgtac cgtacgctca agaatgacgc tgaagttttt ccggatgaat
     6661 gccgtgttag cctgcgccag ctgcagtgct gtgttaatac ccagcgcatt cagcttttcc
     6721 gtcagtctgc gtcctgttcc ccagacttca ccaactggct gcagccccag tagcttcaag
     6781 atccgattac ggttttctgc cgtcagcgcg accacaccgg aaaactgtgg ccattgcttt
     6841 gttgcccact gtgcactttt agccagcgtt tttgtaggtg caatgcccac ccccatggtg
     6901 agtcctgtcc agctctttac ctgttccctg agctgatgac caaaaacctc cggagagatg
     6961 caacggttca cctcccgcaa atcaatgaac atttcatcaa ttgagtaggg ctcaactgcg
     7021 ggagaaagcg actccagaac agccatgacc cgttggctca tgctgtggta cagcgcataa
     7081 ttgctggaaa atacatgtat tttcttctcc aggcgcatct gtctgatctg aaaccagggc
     7141 tgccccattc tgatgccaag ggcttttgcc tccgggctgc gcgcgatcac acagccatcg
     7201 ttattgctga gtacgatgac cggttcgttg cgaaggtccg ggcggaaaac tttttcacat
     7261 gaggtgtaga aactgttgat atcagccagt gcaaacatca gcgtaactcc cgcgtcctgt
     7321 gtatcacgtg agtgacaaca ccaaaaatac agatgttttc cggatacagt gtgcggaa
//
LOCUS       DAZGBC010000202         7271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGBC010000202 DAZGBC010000000
VERSION     DAZGBC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.203"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..235
                     /locus_tag="ACHK1J_004452"
                     /pseudo
     CDS             29..235
                     /locus_tag="ACHK1J_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(324..1460)
                     /gene="yibH"
                     /locus_tag="ACHK1J_004453"
     CDS             complement(324..1460)
                     /gene="yibH"
                     /locus_tag="ACHK1J_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7913635.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1463..1825)
                     /gene="yibI"
                     /locus_tag="ACHK1J_004454"
     CDS             complement(1463..1825)
                     /gene="yibI"
                     /locus_tag="ACHK1J_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7913636.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2362..4275
                     /gene="mtlA"
                     /locus_tag="ACHK1J_004455"
     CDS             2362..4275
                     /gene="mtlA"
                     /locus_tag="ACHK1J_004455"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO7913637.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMFLAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGHK"
     gene            4505..5653
                     /gene="mtlD"
                     /locus_tag="ACHK1J_004456"
     CDS             4505..5653
                     /gene="mtlD"
                     /locus_tag="ACHK1J_004456"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7913638.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDANAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5653..6240
                     /gene="mtlR"
                     /locus_tag="ACHK1J_004457"
     CDS             5653..6240
                     /gene="mtlR"
                     /locus_tag="ACHK1J_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO7913639.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPHFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6252..6461)
                     /gene="yibT"
                     /locus_tag="ACHK1J_004458"
     CDS             complement(6252..6461)
                     /gene="yibT"
                     /locus_tag="ACHK1J_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO7913640.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6746..7108
                     /gene="yibL"
                     /locus_tag="ACHK1J_004459"
     CDS             6746..7108
                     /gene="yibL"
                     /locus_tag="ACHK1J_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO7913641.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVDAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         1843 a         1817 c         1840 g         1771 t
ORIGIN      
        1 atctatagat taaatatttt ttaaatgtga ctgtcatcct tcagataata aagccatcat
       61 cgcaatagat ggaaaagcac tcctgctctt ttttgattag agccgtcgca ggaggaacaa
      121 ctcatatcat cattgcgatc tcgacaatgt atagtctgat ccttcggcag atcaagacgg
      181 ataaaaaatc caatgagttc acactcatcc cgaacttctt aacataatgg atattaacga
      241 aaaatcatca cagccgatga aataatatgc aaaaataatt agtccttaca gggagcaaag
      301 ttccataatc tcccccttcc ccctcaatga tccaaataaa gataatgcat ccagctggtc
      361 attcttagca gcacttttcg catcaccgaa acatggctga aatggtcgga gtaaactgcc
      421 acctgggcgt aaatgccgtc gggtaaggca tcgatatcat cgttagggtc cagttcaatg
      481 gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac cccctgcgcc
      541 tgataagaac cgcctggcac gacaggtaaa atactggtca gtttgccgtg gaacacctgc
      601 ccaggtagcg cgttaaacac cacttccgca tcatcgccag gtttcagacg taacagcgag
      661 ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaaaac catcaccgga
      721 cgcaacggca aggcagctgc gtatgtgcct gggcggatca gtacctgagt gacgtagcca
      781 ttgcttggtg cgcgaatgac agtctgctca agattatatt ttgcttcagt aagttgcgct
      841 cttaagctca caatctgcga ttgctcgccg ttgaccatac tgtcgagctg actctggatc
      901 tgcgcctgct ccgccaccga gcctttcact aacgcatcct gcgcgaggaa attttgccgc
      961 gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc tttcaggtaa
     1021 cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt gttggcctgc
     1081 gcttcagtga gctgggcgcg cagcgttttt atattatgcg tcgccgtcat caggtcagcc
     1141 tgaagtctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag cacctcgccc
     1201 ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt cacctgtggc
     1261 gtgataggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg gttgtagttc
     1321 atcaacaaaa tcaaaccact caccagaaac acgcctccca gcgccgccgt cgccagcgtc
     1381 cactgattca ccggaatacg aaagatttta aagaccgccc acgccagcgc cacgtaagtt
     1441 aaaacaatca atagatccat aattagatct ccgggaacgt ggaatagtca gtctttttct
     1501 cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgcggcg atttccgggt
     1561 cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc tccggttgat
     1621 agagcgtcgc ccagatccac agaaacggcc agataacatg cagagtaaac aggctcaccc
     1681 agcccgccgt atgaatagcg tcggcatggg gatggttgcg ctttttggca atcagataag
     1741 ggatgtcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag atcagcactc
     1801 ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca gtgttgtttt
     1861 ttggctggca gccagaaggg agtcaggctg atattttgac aataatccgg gttcgcgatt
     1921 ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt acataacgat
     1981 tgtatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta agaaaatatc
     2041 ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga cattactcac
     2101 atttaaatgc catttttagc gaaaatcgcc tccttgttgc ttttttacac aagcgttttg
     2161 tgatgaacgt cacgtcaatt acctctctac cccctatatt tatgtgattg atatcacaca
     2221 aaaggccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta tctacaaggt
     2281 ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg cgcgacagca
     2341 aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca aagctttggt
     2401 cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc
     2461 accgcgttat ttattccaac agggtggtta ccgaacgaga cgctggcgaa gctggtcggg
     2521 ccgatgatca cttatctcct gccgctgctg atcggttata ccggtggtaa gctggtaggc
     2581 ggcgaacgtg gcggcgtagt cggtgccatc accaccatgg gcgttatcgt cggcgcagac
     2641 atgccgatgt tcctcggttc tatgattgca ggtccgctgg gcggctggtg cattaagcac
     2701 ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt gaataacttc
     2761 tccgcaggca tcatcgggat gttcctcgcc attctggcat ttctcggcat tggcccgatt
     2821 gttgaagccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt ccatgacatg
     2881 ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa caacgccatt
     2941 aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg taaatccata
     3001 ttcttcctga ttgaagctaa cccaggtccg ggtatgggcg tgctgctggc gtacatgttc
     3061 tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg caattatcca cttcctgggt
     3121 ggtatccacg aaatttactt cccgtatgtg ttgatgaatc cgcgtctgat cctggccgtt
     3181 atcctcggcg gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg tctggtttct
     3241 ccggcatctc cgggttctat ccttgctgta ctggcgatga caccaaaagg tgcttatttc
     3301 gctaacatcg cgggtgtgtg tgcggcgatg gctgtctcct ttgttgtctc tgctattttg
     3361 ctgaaaacca gcaaagtgaa agaagaagat gatattgaag cagcaactcg tcgtatgcag
     3421 gacatgaaag ctgagtctaa aggtgcatct ccgctgtctg cgggcgatgt gactaacgac
     3481 ctgagccacg tacgtaaaat catcgttgcc tgtgacgccg gtatgggttc aagtgcgatg
     3541 ggcgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc tgtcgcagat ttcggtcact
     3601 aacagtgcga tcaacaacct gccgccagat gtggacctcg tcatcactca ccgtgacctg
     3661 accgaacgcg ctatgcgcca ggttccgcag gcacagcata tttcgctgac caacttcctc
     3721 gacagcggcc tgtacaccag cctgaccgaa cgtctggttg ctgcccagcg ccatactgaa
     3781 aacgaagtga aagtgaaaga cagtctgaaa gacagctttg acgattccag tgctaacctg
     3841 ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag cggccaccaa agaagaagcg
     3901 attcgttttg ctggcgagca gctggtgaaa ggcggttacg ttgagccgga atacgttcag
     3961 gcgatgctgg atcgtgaaaa actgaccccg acttatctgg gtgagtctat cgcggtgcca
     4021 cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg gcgtggtgtt ctgccagtac
     4081 ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg cccgtctggt gattggtatt
     4141 gctgcccgta acaacgagca cattcaggtt atcaccagcc tgaccaatgc actggatgat
     4201 gagtccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct ggaactgctg
     4261 gcaggtcata agtaatccaa tcccaccctc tccacatgga gaaggtgggg ctaattgcct
     4321 gatgcgctac gcttatcagg cctacaggat gcatcacaat ttgttgaatt tgcacgttct
     4381 tgtaggccgg ataaggcgtt tacgccgcat ccggcgcggc ccctctccac atggagaggg
     4441 tttgggtgag ggaaaagcct caccccagcc ctctcgggta aaaacattga tgaaggttaa
     4501 tactatgaaa gcattacatt ttggcgcagg taatatcggt cgtggcttta tcggtaaact
     4561 gctggcagac gcgggtatac aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc
     4621 cctgaatgcc cgtcatagct atcaggttca tgtggtaggt gaaaccgagc aggtagatac
     4681 cgtttccggc gtcaatgctg tcagcagcat tggtgatgat gtcgttgatc tcattgctca
     4741 ggttgattta gtcactaccg ccgttggccc ggtagtgctg gaacgtattg ctccggctat
     4801 cgccaaaggg ctggtgaaac gtaaagaaca aggtaatgaa tccccgctga acatcatcgc
     4861 ctgtgaaaac atggtacgcg gtaccacgca gctgaaaggc catgtgatga acgccctgcc
     4921 ggaagacgcc aacgcgtggg tagaagaaca cgttggcttt gtcgattccg ccgttgaccg
     4981 catcgtaccg ccttcggctt cggcaactaa cgatccgctg gaagtgacgg tagaaacctt
     5041 cagcgaatgg attgtcgata aaacgcagtt caaaggtgca ctgccgaaca tcccaggcat
     5101 ggagttaacc gacaacctga tggcatttgt cgaacgtaaa ctcttcaccc tgaacacggg
     5161 tcatgctata accgcgtacc tcggaaaact ggccggtcat cagaccattc gtgacgctat
     5221 tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg gcgcggtact
     5281 gatcaagcgt tacggctttg acgctgacaa gcatgcggcg tacatccaga aaatcctcgg
     5341 ccgttttgag aacccgtatc tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg
     5401 taaactgagc gcaggcgacc gtctgatcaa gccactgctc ggtacgctgg aatatggtct
     5461 gccacataaa aacctgattg aaggtattgc cgctgcaatg cacttccgca gtgaagatga
     5521 tccgcaggct caggaactgg cagcactgat cgctgacaaa ggtccgcagg cggcgctggc
     5581 acagatttcc ggtcttgatg ccaacagcga ggttgtatct gaggcggtaa ccgcttataa
     5641 agcaatgcaa taatggtgga ccaggcgcag gacaccctgc gcccgaataa cagattgtca
     5701 gatatgcagg caacaatgga acaaacccag gcctttgaaa accgtgtgct tgagcgtctg
     5761 aatgctggca aaaccgtgcg aagctttctg atcaccgccg tcgagctttt gaccgaggcg
     5821 gtaaatcttc tggtgcttca ggtattccgc aaagacgatt acgcggtgaa gtatgctgta
     5881 gaaccgttac tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt
     5941 tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg cggaactgct gatggcattg
     6001 cgtgaagagc taaatcacga cggcaacgag tacgcgttta ccgacgacga aatccttgga
     6061 ccctttggtg aactgcactg cgtggcggcg ttaccaccgc cgccacattt tgaaccagca
     6121 gactccagtt tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg atcaacaatg
     6181 gtcctttcac tgacagagct gatttccaaa atcagcttaa aaaaagcctt tcaaaagtaa
     6241 gcaacgtctg cttactgccc ctctacctgc ttcggccgat aaagccgacg ataatactcc
     6301 agacgttgta gatataacgg cacgctttca tcgggtattc cggacggaat cgcgttacgg
     6361 ggaggaagtt ttttcagata ctcccggaac gcctggcttg atgccatgaa atctacggct
     6421 ttatcgataa gaagcggaac gttttcacct aatttgccca tgatgatatc tccgtattac
     6481 cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa gatgacatta
     6541 aggaaaacgt gctgaatcct caataatcat ccgcttctgt ttattatctt ttttctatag
     6601 atttcttaat taatcaataa attgcgtagt ttttaagcat attaatgatt acgttgcgga
     6661 actattgcaa tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta ggggctataa
     6721 atttatcttt atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct
     6781 cagcgatcgt ctggacgcca tccgccacca gcaggccgat ctgtcgctgg ttgatgccgc
     6841 agacaaatat gccgagctgg aaaaagagaa agccacgctg gaagcagaaa ttgcccgcct
     6901 gcgtgaagtt cacagccaga aactgagtaa agaagcacag aagctgatga agatgccgtt
     6961 tcagcgcgca attaccaaaa aagagcaggc tgatatgggc aagctgaaga aaagtgttcg
     7021 cggactggtg gttgttcacc caatgaccgc gctgggccgc gaaatgggcc tggaagagat
     7081 gactggattc tctaagactg cgttttaagc ccctcagacg attggtacga tcgtatcaac
     7141 ccgcatctgg cttgcgttag ctggtcaatg cggggactcc cctgtctgct cgcctaatta
     7201 ctgaacaatc cactccctct acatcaattt accgctggtt gatattctga aaatatattt
     7261 ttaccgaaat a
//
LOCUS       DAZGBC010000203         7268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGBC010000203 DAZGBC010000000
VERSION     DAZGBC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.204"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(147..431)
                     /gene="ppnP"
                     /locus_tag="ACHK1J_004460"
     CDS             complement(147..431)
                     /gene="ppnP"
                     /locus_tag="ACHK1J_004460"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO7913642.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(503..1180)
                     /locus_tag="ACHK1J_004461"
     CDS             complement(503..1180)
                     /locus_tag="ACHK1J_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO7913643.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1438..1629)
                     /gene="yaiA"
                     /locus_tag="ACHK1J_004462"
     CDS             complement(1438..1629)
                     /gene="yaiA"
                     /locus_tag="ACHK1J_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO7913644.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1679..2203)
                     /gene="aroL"
                     /locus_tag="ACHK1J_004463"
     CDS             complement(1679..2203)
                     /gene="aroL"
                     /locus_tag="ACHK1J_004463"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO7913645.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2386..2844)
                     /locus_tag="ACHK1J_004464"
     CDS             complement(2386..2844)
                     /locus_tag="ACHK1J_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO7913646.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2964..3773
                     /gene="proC"
                     /locus_tag="ACHK1J_004465"
     CDS             2964..3773
                     /gene="proC"
                     /locus_tag="ACHK1J_004465"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO7913647.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRSQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(3790..4905)
                     /gene="adrA"
                     /locus_tag="ACHK1J_004466"
                     /gene_synonym="dgcC"
     CDS             complement(3790..4905)
                     /gene="adrA"
                     /locus_tag="ACHK1J_004466"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO7913648.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(5007..5327)
                     /gene="psiF"
                     /locus_tag="ACHK1J_004467"
     CDS             complement(5007..5327)
                     /gene="psiF"
                     /locus_tag="ACHK1J_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO7913649.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5446..6861)
                     /gene="phoA"
                     /locus_tag="ACHK1J_004468"
     CDS             complement(5446..6861)
                     /gene="phoA"
                     /locus_tag="ACHK1J_004468"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO7913650.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFNGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(6962..7222)
                     /gene="iraP"
                     /locus_tag="ACHK1J_004469"
     CDS             complement(6962..7222)
                     /gene="iraP"
                     /locus_tag="ACHK1J_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO7913651.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1777 a         1896 c         1735 g         1860 t
ORIGIN      
        1 cataatcttc aatgatattt atatcaacct tgatgaatta tgaatatatc ggaaattgaa
       61 aaaatcgcag caaaggaaat attattatgg gttaaagaga ggatatttca gaaaaatgcc
      121 ctcccgttca aggggaaggc gaggaattac agatagcggc acagataaga ggtgggttcg
      181 gcaacttgca gatgaaactc actgtgaccg ggaacattaa acaccgaacc ggcttcatac
      241 acttgccagt cggtcgcgtc aggcagtaac acattcagcg cgccactgat taccgtcatc
      301 tcttccggct cagcggtgct gaaggtgtat tcgccttcaa ccataacacc cacgctggcg
      361 cgaccagtgc tgctgctgga aaagccgatc gatttcactt tgccggaaaa gtactcatta
      421 ctttgaagca taaactggcc ctgtctggta gttgaacatc tacttcacta taggggccag
      481 aggcgctggc tgtcacgcaa aattacacca gtaattccgc agccaaccgt gcaatcaata
      541 cgttagagag caagacagga acatcgagct gtttttgcag taaatcgcga tgacgctggt
      601 taaatcccaa acaatccagc atgatgacat ctgcgccttt cgccagtaat tctttcccgg
      661 catcaatgat tttttgttct gaatcatgaa tggggttacc caatgaaaat accggcggtt
      721 tctgcaaaat ttgccatttt tgcgcctgaa cggtcagcaa ctcctcaacc ggtacgataa
      781 cccccacctg atgatcttca acaatagagg aaaccagcgg tggcaatatc cgcgacggct
      841 caagaaagat cgtattacgc gcagtcatac tactaatgtt tgccgtactc attaatataa
      901 tgacgtcgta gccctgatta tcgagcactt caaccacacc ttgcaggtca cgctctactt
      961 tgcgacgcga aacatgggcc agatggttgt catttaataa tgtgagaatt gtgtcttcgc
     1021 ctgcttctgg cgcgtactcc gccatcactt cttcgcggct taacttcccc agcaggctat
     1081 gatgggaaat attatcttcg tcaatgtatt ctgtcaggag cggcaaaact tcctgcatag
     1141 gtacaatgcc gatggttaga atcgccaacg acgcactcat tgttcttcca ccttactttt
     1201 atttcgtcgt taattaccgg gcaagtgtta agcaccactg ctgacactta cttgtgctac
     1261 aaatgacaaa aagcgtagca gcagacgtgc acggcataat gtaaagattc caaatgattc
     1321 cagtaatgga ttgttattgt ttaatattct aattatttga aaaacatgaa ttatgaaaaa
     1381 tgtgacgcag atcccttgta gtggcactca gaatcccttc cgagcagtct gatgcggtca
     1441 ctctttgtca gggtcctcat agcgtttttt cgcatccatc gcttcctgag cggtaggatg
     1501 ttcactgatc aacgagtctg gtttaggatg atcggctctg agttggtacc aggttaccgt
     1561 ctgtcctggt tttccttttt caatcgtcac tatatatgct tcacgaggat aaggcggttt
     1621 cgttggcatc gttcttcctt atttcacggg atgaacgtta agtataggcg ctctaaaatc
     1681 aacaattgat cgtctgtgcc agggcgctac gaatttcaga aatcacctgg ctgggttcgt
     1741 ttgttgcgtc gatgataata tgcgcaactt cgcgatatag cgcatcgcgt tcttccagca
     1801 cttcctgaac ttcttcgctc agaggttttc ccgttaaggt tggccgtaaa tcttcttccg
     1861 gtgcagcttg cagtcggtta accaggactg aaactggcgc acacaaataa accacgatcc
     1921 cgttattttg catgaagtga cgattaaatt ccgtcagaat aatgccgccg cctgtagcga
     1981 taacggtgga tggcgcagtt accgcttcca gcgccgccgt ttctctggcg cgaaatcccg
     2041 cccactcttc cctttcgacg atctccgcga ccgtcatatt gagctgtgat tgcaaccact
     2101 gatcggtatc gacaaaccga cggttaagcg aatcggcaag ggccattccg accgttgttt
     2161 taccacagcc ccgaggcccg atcagaaaaa gaggttgtgt catcgtgggt tttccccaat
     2221 aggtcgcaat gcggcgaaag ccggtgtcat gagaatagcg atcataccat caaactagta
     2281 caatttcgat tgtaaagaaa aaattccact taaagtgaaa atctcaatac atcccttact
     2341 ataccaataa atattcaaga atgaagtgta aataataaat tacatttagc cacgactacg
     2401 ttgcacttcc agccaccact tctccagctc cgcggcaaat gcctggcggt cacgttgtga
     2461 aaggctatct ggtccgccgg tctggatccc actggcacgt aaggtatcca taaaatcgcg
     2521 catcgtcagg cgctcacgaa tggtcgctgg cgtgtaacgt tcaccgcgcg gattaagcgc
     2581 cgcagcgcct ttctcgatgg cttcagcagc caaaggtata tctgcggtga tcaccaaatc
     2641 gcccgcttca cactgccgga caatttcgtt atcggcaacg tcgaaacctg ccgcgacgcg
     2701 cagcgtacga ataaatcgcg atggcggcac gcgtaaactc tggtttgcta ccagtaccag
     2761 cggcatctgc atacgttccg ccgcgcgata caaaatctct ttaattacat tgggacacgc
     2821 gtcggcatcc acccaaattg tcataaagtc atcctttgtt gggtaatcct ctattgtgtc
     2881 gcgcttttgc cttccggcat agttctgttt atgcttctgc cagcgattat caaaacaatg
     2941 aatttcacgg caggagtgag gcaatggaaa agaaaatcgg ttttattggc tgcggcaata
     3001 tgggaaaagc cattctcggc ggtctgattg ccagcggtca ggtgcttcca gggcaaatct
     3061 gggtatatac cccctccccg gataaagtcg ccgccctgca tgaccagttc ggcatcaacg
     3121 ccgcagaatc ggcgcaagaa gtggcgcaaa tcgccgacat catttttgct gccgttaaac
     3181 ctggcatcat gattaaagtg cttagcgaaa tcacctccag cctgaataaa gactctctgg
     3241 tcgtttctat tgctgcaggt gtcacgctcg accagcttgc ccgcgcgctg ggccatgatc
     3301 ggaaaattat ccgcgccatg ccgaacactc ccgcgctggt taatgccggg atgacctcgg
     3361 taacgccaaa cgcgctggta accccagaag ataccgctga tgtgctgaat attttccgct
     3421 gctttggcga agcggaagta attgctgagc cgatgatcca cccggtggtc ggtgtgagcg
     3481 gttcttcgcc agcctacgta tttatgttta tcgaagcgat ggccgacgcc gccgtgctgg
     3541 gcgggatgcc acgctcccag gcgtataaat ttgccgctca ggcggtaatg ggttccgcaa
     3601 aaatggtgct ggaaacggga gaacatccgg gggcactgaa agatatggtc tgctcaccgg
     3661 gaggcaccac cattgaagcg gtacgcgtac tggaagagaa aggcttccgt gctgcagtga
     3721 tcgaagcgat gacgaagtgt atggaaaaat cagaaaaact cagcaaatcc tgatgacttt
     3781 cgccggacgt caggccgcca cttcggtgcg gttacgtccg gctttctttg ctttgtagag
     3841 cgccaaatct gccgatttca accactcacg atagtgactc atttgtgggt tcagcggcgc
     3901 aacccccaca ctaatccgta aagttacctg tggcgtattc ggcaaacgta atgtatttag
     3961 cccttcatgc acccgtaaca tggcggtaat ggcgctctca gctggcgtac cggacatgat
     4021 tactgcaaac tcatcgccgc caaaccgacc aatcacatcg ctaccacgca gggtaatttg
     4081 taactgtcgg gtaagcgcca caatcgcttc atcgcccaca tcatggcccc aggtatcgtt
     4141 gatgctcttg aaatggtcga tatcgataat cagtaacgtt gcatcgcgat tatgccgccg
     4201 acagttatca aattcattgc gtaacatagt ttcccaatga cgtcggttat acacgcccgt
     4261 catgccgtcg cgggtactca tgacctgcaa cctgcgttta tgttccgcca gtttagtcgc
     4321 cgtctggtag ctgacccagc caaacagcag aggataaatg acaataatgg gaagggagag
     4381 ccaccattcc agcggcgcac tattgaacga cacggtgatg cccgtcagct cgagggtgac
     4441 aaggcaggaa accaccatca acaccagacc cgcgacaaac agacgggggc cgcctgcccc
     4501 catcaaattc agacacataa tcatcaacat cgcggtggaa ggcagcacgt ttacgcccat
     4561 tacgcctacc cacattcccg ctaatactgc atcggttttt aagttgtaaa tttcccggct
     4621 aagcggatcg acggccctgc tcgctatctg ccaggctaaa tgcggccaga cgaacgccca
     4681 gccgaccaac accagccacc accagcccgg cggcgggtgt gaaaccagcg ttgaagcaat
     4741 cggtaagaac atgccagcca ggccaaccgc acggggtagt ctgacgcgac gggcgaagcg
     4801 cagcccggac cgctgatgtt cgttttgagg agttaaagga ggttcctccc cgtgcgccgc
     4861 cgcttttttg aaaaagtttt catcattcat tatttttggg aacattcaga aacaattttc
     4921 ccaaattata gagacggggc taaaggcaga agtatgaaat ttcgggtgag ccattttagt
     4981 agctcacccg ttagcactat caggtatcag gcggctttct tgaggcaggc gctcataaac
     5041 ttattacgat catcaccttt cagtgattgt tgcgttgctt gattattaca ttcgcgcatc
     5101 ttttgctgct gtggtgtcaa acttttttca ccaggcgcag acttgctgtt cttcaggcaa
     5161 tcactcatgt aggtcttacg agcatcccct ttcaacgcct gtgccgtcgc ctgctgatta
     5221 caggaggtca tacgctgttg ttgtggggtt aaggttctct cggcagcgcc gacggtggtt
     5281 aaaaaaacca gaccgaaaag caaggtaacc agtaatgtaa ttttcatagc accatccctc
     5341 ttcatgtttt aaccatgagc gtatgcgccc gtgatctgcc attaagtctg gttgctaaca
     5401 gcaaaaaaac cacccggcag cgaaaattca ctgccgggag cggttttatt tcagccccag
     5461 agcggctttc atggtgtaga agagatcggt ctggtcggtc agtccaacga cattggcggc
     5521 atgcgggcca tacgccgcaa tacgcaactg actgccggta tgttcttgtg aatcctcttc
     5581 ggagttcccg taactcatca ccatcactgc gccatctttg gtatttagcg cctgggtgag
     5641 gcccggagct ttggtatccg gcgcaacaat ctggctggcg tgggcgtgat cagcggtgac
     5701 tatgaccagc gtgttaccat cctttttggc gaattccagc gcccgttgta cggcttcatc
     5761 gagatcgacc gtctcgccaa tttgcccaca aggattcgca gcatgatcct gtttatcgat
     5821 tgacgcacct tcaacttgca ggaaaaagcc tttctcattt ttactcaata attcaatggc
     5881 tttgtcggtc atctgtgcca gggtcggtac actgtcatta cgttgcggat taggcgtaca
     5941 ggtgactgcg ggcttatcga tattaccgtg gtacgttgct ttcggtccta gccagcgcac
     6001 tggcatattg ccgtcagcaa acaggccaag caggggtttt tgctgattcg cttccgtcac
     6061 cgaattcagt gaggcagcat cgctcaccaa ctgataacca cgcgcctgtg cctgttcacg
     6121 cagcgttttt ccctgccatt caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc
     6181 gccaagcgta acgtcggcac gagcgttaag cagctgttcg gtaatcgatc cttttccgcc
     6241 tttttccaga gcgttacccg gacatttttc actggtcgcg ctcggaccgt agcatttgcg
     6301 cgaagtcaca tgtgccacca gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac
     6361 gttaccggtc gccagacctg cggcttttgc catttccaga atcgttgggt gatctttttc
     6421 gtgaatatcg acgcccagcg cgccgttata ggttttgaca ccggttgacc aggcggttgc
     6481 tgatgcagcc gagtcggtga cgtagtccgg tttgccggtt tttttattca gcgcatagtg
     6541 agtgtattgc ccggtaagcg gtaaggcatc gataccatta aaaaagccgc ccgcaccttc
     6601 ggcataatta cgtgcggcag taatttccga atcccccatc ccatcgccaa tcagcaaaat
     6661 aatatttttt gcaggtttat cgctaagaga atcacgcaga gcggcggtct gatcagcagt
     6721 taagcggcga gcaccgccgg gtgcagtaat atcgccctga gcagcccggt tttccagaac
     6781 aggcatttct ggtgtccggg cttttgtcac aggggtaaac agtaacggta agagtgccag
     6841 tgcaatagtg ctttgtttca ctttattttc tccatgtaca aatacattaa aaaataaaaa
     6901 caaagcgact ataagtctcg gccgtgacaa ctttatgaca tctgttgaaa agattaactt
     6961 tttactgacg aggatgcttc aataacttct ttacgtaatc gcgcagcagc tccgtatcgt
     7021 cgtcaggaat gctggcatcg ggctttacct cgtacagcgc cccctctacc tgatcaatca
     7081 accgttgttg gtcattttgc gccatattgc gaagcattgc agtgacgata atctccaaag
     7141 cttctacctg cgcacacagt tctttcgact cttcttcttt ttgggcaagc ttaaataaca
     7201 actcagcaat gagatttttc atgtctgtat ttccttatcc aaagtatgga gaagttaaca
     7261 ttatgttt
//
LOCUS       DAZGBC010000204         7215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGBC010000204 DAZGBC010000000
VERSION     DAZGBC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.205"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(61..663)
                     /gene="fimB"
                     /locus_tag="ACHK1J_004470"
     CDS             complement(61..663)
                     /gene="fimB"
                     /locus_tag="ACHK1J_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO7913652.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2117..2833
                     /gene="nanC"
                     /locus_tag="ACHK1J_004471"
     CDS             2117..2833
                     /gene="nanC"
                     /locus_tag="ACHK1J_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO7913653.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2853..3959
                     /gene="nanM"
                     /locus_tag="ACHK1J_004472"
     CDS             2853..3959
                     /gene="nanM"
                     /locus_tag="ACHK1J_004472"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO7913654.1"
                     /translation="MNKTITALAIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALASFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRDNYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4024..5004
                     /locus_tag="ACHK1J_004473"
     CDS             4024..5004
                     /locus_tag="ACHK1J_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7913655.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDALHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALTKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(5012..5662)
                     /locus_tag="ACHK1J_004474"
     CDS             complement(5012..5662)
                     /locus_tag="ACHK1J_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7913656.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIDQGFHIHQDKAAAAPIADVLFPD
                     ELPSQGTYVEGAAIKVTVNSYERDKKAREKAVEYHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYLLDPVNDLLPVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            5799..6671
                     /locus_tag="ACHK1J_004475"
     CDS             5799..6671
                     /locus_tag="ACHK1J_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO7913657.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIVLPQVRKNYPY
                     EGFIEWHRKEVFRG"
     gene            6716..6895
                     /locus_tag="ACHK1J_004476"
                     /pseudo
     CDS             6716..6895
                     /locus_tag="ACHK1J_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         2129 a         1550 c         1567 g         1969 t
ORIGIN      
        1 ttttgatata tcgtaagaat aatgtagttt ttaacaccat ccctggtatc tcaactatct
       61 ctataaaaca gcgtgacgct gtcgtcctct ggctctatcc cagatgccgt aaaaacgccc
      121 tgcattgctg gcggtatacc agacagtatg acgaatattg cgatgcccaa gataatcctg
      181 gataagtcgc gtatctattc ccatattcgc caaagcaaaa ccacacgaat ggcgtaacat
      241 gtgcggatga atctccagtg acaacccggc attaccaccg gaagtcgaga taatatggta
      301 aaactgttgc cgagaaagcg gattcccctt acgcgataaa aatacccact cgctctcagc
      361 atgcgggtac gaagtacgga tactcaacca gttttttaaa gcctgaactt ctttgttcaa
      421 tagcgggtgc gttgttgaaa agcctttttt taatcgatgg atatatatac actttgcctt
      481 aagatcaata tccgaaatcc tcaatcgaca aatttcactc gcccggaaac catgaataaa
      541 acaaagcaaa gtcagacaat aattacgtgc tgcatgaggc ccggtatttg ctgctttaag
      601 gagtgattcg atttcactat gggtcaggaa gttccttttt ttgttatcag ccttattctt
      661 catcgttttt cccttataat tacagacgcg cactagctgt gctgggttag caataatcca
      721 acatttttat cctgattatg tttataaaag cgaacgtttg cttaataact aacgtaagtg
      781 gaccagttct tctgagtgaa cttaaatgga ttagcaacag ttaattataa taacgttgcc
      841 atacaaacgc tatgctcttg catgctatgt acctttatat atttatcaat cggatacaaa
      901 tctaataata accctcatca ttaatgcaac taatcttatc tatatatcat gtgatatgtt
      961 ctgtaacaag taaaatcaac ataaaaaatt cagcaaaatt taatattcgt tcaactagcc
     1021 gtctcacata tgacattatt agtcaactcc atttgccaca aaatggctat ttcatggaac
     1081 gcagtaattt cttagtggct attatcatgc taattcttag gtccctagcg attattcctg
     1141 gctggttcgt cagtttaggc catgagattg ttacattcct ttttatcatg gagatatggg
     1201 ggaaagcagg aaacgatttt ttcatctaca ttcgttggta aatatagctt tggtcactac
     1261 gacttaaacc ttcctcaatc acagccattc gccgggcgat acggcaaaca aactatctga
     1321 aacatcatcc tgaaaacacc aacatcaaca agcctctcca gatcgacttc agaagtgacc
     1381 agttacaagc cacaaaccag gaaacatatt tcatgatgag aattatgctc agcctgatgg
     1441 cgggtaaaca cttattttca ccacgcccac tgagttagct tgcaaagctt cccttgttta
     1501 gactctaaat aaattattta taaaacaaat agttaaatga agatctttag tttttaaaga
     1561 ccaccgcaag tgttcgtctg gcttcacatg gcatcttctt cttagaaaaa gatcgacata
     1621 ttttgtgaca cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt tttgatctgg
     1681 tataacaggt ataaaggtgc accacaatag acaatgagac agggcatctt gcaatctatg
     1741 gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag tctgaaagga
     1801 atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat ggattgagta
     1861 agtgatatag cttacgaaca ttaaaatcaa ttaaacatca gaagagattt tatactcagg
     1921 tatttaatct ggctctctgt ttatttaaat aacgtggaaa gagatttttc acaggagaca
     1981 ttatacaaaa aatataaaat acagctaccg gttgccaaag acactataag cctggcaaaa
     2041 atattacaca acataaatgc taattgttta tgcgggcttt gtattgcttt ctgtatccta
     2101 caaatgagtg aaatttatga aaaaggctaa aatactttct ggcgtattat tactgtgctt
     2161 ttcgtcacca ttaatttctc aggctgcgac acttgacgta cgtggtggat atcgtagtgg
     2221 aagccacgcc tatgagactc gactcaaagt cagtgaggga tggcaaaatg gatggtgggc
     2281 aagcatggaa agtaatacct ggaataccat tcatgataat aaaaaggaaa atgccgcact
     2341 caatgatgtt caggttgaag ttaattacgc gattaaactt gatgatcaat ggacggtgcg
     2401 cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc tacggaccct acgtaaaact
     2461 gtcctgggac gcgacaaaag atcttaattt tggcattcgc tatcgttacg actggaaagc
     2521 ttaccgacaa caagacttat ccggtgatat gtctcgtgat aacgttcatc gttgggatgg
     2581 atatgtcact taccatatta atagtgattt caccttcgca tggcaaacga cgctatacag
     2641 caaacagaac gattatcgct atgcaaacca taagaaatgg gcgacggaaa atgcatttgt
     2701 tctacaatac catatgacgc ccgatattac gccatacata gaatatgact acctggaccg
     2761 tcagggtgtt tacaacggca gagataattt atcggaaaac agttatcgca ttggtgtgtc
     2821 atttaaactg tagtagacag gagacagtca caatgaataa aacaataacg gcgcttgcta
     2881 tcataatggc ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct gtgccattta
     2941 aaagtggtac cggagcaatt gataacgaca ctgtctacat tggtttaggt agcgcaggta
     3001 cggcatggta caagctggag acacaggcca aagataaaaa atggacagcg ttagcttcat
     3061 tccctggcgg accaagagat caagcaacct cggcatttat tgatggcaat ctgtatgtgt
     3121 ttggcggcat tggcaaaaac agcgagggct tgactcaggt atttaatgac gtacacaaat
     3181 acaaccccaa aaccaatagc tgggttaaat tgatgtcgca cgcaccgatg ggcatggcgg
     3241 gtcatgtgac ttttgtacac aacggcaagg cttatgttac tggcggtgtt aaccagaata
     3301 tcttcaatgg ctattttgaa gatctcaacg aggctggaaa agattcaacc gctatagata
     3361 aaatcaacgc tcactatttt gacaaaaaag cagaagatta tttcttcaat aagtttctgt
     3421 tgtcttttga tccctcaacc cagcaatgga gttacgctgg cgaatcgccc tggtacggaa
     3481 cggctggtgc ggcggttgtg aataaaggtg ataaaacctg gcttattaat ggtgaagcca
     3541 aaccaggatt gcgaacggat gccgtatttg aacttgattt caccggtaat aatttaaaat
     3601 ggaataggct tgctcccgtc tcatcaccag atggcgtcgc tggcggtttt gcggggataa
     3661 gcaatgattc tcttatattt gccggagggg ccggattcaa aggttcacga gataattacc
     3721 agaacggtaa gaactatgcg catgaagggc tgaaaaaatc atatagcgct gatattcatc
     3781 tttggcataa cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg gcctacggag
     3841 tatcattgcc ctggaataat agtctattga ttattggcgg tgaaactgca ggcggcaaag
     3901 cggtgacgga ttcagttttg atctctgtga aggataacaa agttactgtg caaaactaac
     3961 gcttcagggc cccagtaaag gggccctgct atcaatttgt catttcaatt acgcgaaaat
     4021 tatatgaacg caataatatc gccggattat tactatgttc ttaccgttgc tggtcagtct
     4081 aatgccatgg cgtatggcga aggactgcca ttaccggacg gggaagatgc gcttcatccc
     4141 agaattaaac aattagcgag atttgcgcat acgcatcccg gaggcccgtc atgtcacttt
     4201 aacgacatta ttccactgac tcactgccca cacgatgttc aggatatgca gggttatcac
     4261 catcctctgg caacgaatca tcaaacacag tacggcaccg ttggccaggc actgcatatt
     4321 gcacggaaat tactgccctt cattcctgat aatgcagggg ttctcatcgt tccatgttgt
     4381 cgtggcggct cggcttttat cgcaggcagc gaagggacat attcagaacg gcacggagcc
     4441 agccatgatg cttgtcgttg gggaacggat actccgctat accaggattt agtcagcaga
     4501 acgcgagccg cactgacaaa aaatccgcag aacaaattcc tcggcgtatg ctggatgcaa
     4561 ggcgaatttg acttaatgac cagtgactac gcgtcacacc ctcaacactt taatcatatg
     4621 gttgaagcct ttcgtaggga tctaaaacaa taccattctc agcttaataa tattactgac
     4681 gcgccgtggt tttgcggcga taccacctgg tactggaaag aaaatttccc tcatgcgtat
     4741 gaagctattt atggcaatta tcaaaataat gttttagcca atattatttt cgtcgacttc
     4801 cagcaacaag gtgaaagagg actgacgaac gcgcctgatg aagatccgga cgatttaagc
     4861 acgggatatt acggttcagc gtaccgctca ccggagaact ggacgacggc actgcgaagc
     4921 agtcatttca gcgcggcagc ccgtcggggg attatttctg acaggtttgt agaagcaatt
     4981 ttgcagtttt ggcgcgaaag gtgagcgttc attaatttga tttattcgca tccatcaacg
     5041 cttttaactc ctccggcgta aacggtggtt tgcgtcggtg taacatcgca tggcaattag
     5101 ggcacacagg aagtaaatca ttaactggat ccagcaggta atcctcttta atggctgaaa
     5161 gcggcaccag gtgatgcata tggataaagc cctcagcgat atcgccatag atcttcacca
     5221 gatcaacgcc gcatacgttg cactggcatc cgtgatattc gactgctttc tcgcgagctt
     5281 ttttatcgcg ttcatagctg tttaccgtca cctttatcgc cgcaccttca acgtaggtcc
     5341 cctgcgacgg caactcatcc gggaacagaa catcggctat gggagccgca gcggctttat
     5401 cctgatgaat atgaaatccc tgatctataa gtagcttgac gcatttagat tttattccgc
     5461 cggtgaaatc cgcaggggta aattgcgtcc ccgtcatcaa tgtcgctgcg atgccagcaa
     5521 tcgccttcga ggggtaacgt cggccatgat attcaatctc atacaaccgt gcaggctgga
     5581 actgatgagt ttctcccgca tcccataaat gaatagcttc gataatatac cgccgcggaa
     5641 gatgatcggg gagtttattc atagagaatg tccttatacg ttctcagaac aagcagcaaa
     5701 aaaacaaatg agatcaaagt aatatatctt cacttgcagg cttgaacagc agaaaatttg
     5761 cgagcatgat cgcaattatt tcactgccag ggatatcaat ggcttccagt aaatcgctac
     5821 agcaggcaat tgccaacata aaaatctggc acaagggtga acagcgcgcg ccgcataaac
     5881 cattgttact gctatacgta ttagcgggat acctgaatgg acatccacgc cttttcgatt
     5941 atggatcgga aatctacgaa cctctgcaca gtttactgga acgttttggg ccacagcgtt
     6001 cacagtaccg acctgacatg ccgttctggc gattacaagg cgacggattc tggcaattac
     6061 acaatgccga gctttgctca acagcaggga gcagccgaca accgccagtg aaggaactga
     6121 acgaatacca cgttgcaggc ggtttcgatg aacagcacta cgccctggta accggcaata
     6181 aaaaactcat caatactctg gcgcaacaga tactcgaagc gcacttcacc gagagtattc
     6241 aggaagaaat cgctgacgag ctgggttttg atctccagca aatccgtaaa cagcgcgacc
     6301 cgttatttcg caaaaatgta ttacgcgctt ataactacca gtgtgcgata tgtggcttta
     6361 atatgcgcca cgacgacacc accgtcgcgc tggaagcggc gcatataaaa tggaaacagc
     6421 acggcggtcc ttgtgaaatt cctaatggcc tggcgctttg cgcaatacac cataaagcct
     6481 ttgataaagg atcgattggc ctggacgaaa atatgcgggt actggtatca gatgcggtaa
     6541 acggcggagg gatcgttgag agattattct gggattttga cgggaaaact attgtgttgc
     6601 cccaggtgcg taaaaattac ccttatgagg ggtttataga gtggcatcgg aaagaggttt
     6661 ttagggggtg aggttgataa ctatcgcagt atgttcaata ttgctgacat aatcagtgca
     6721 ggatcttgcc gctagtttac aattcgcctt cgatagcggc gcaaaacgag ttctgttgcc
     6781 gatatcctcg gctatggata ttccaacagt tccggcagag ttgtttacca agtttcaggt
     6841 aagtttttac tcagaaccgg ttgatgcggt ttataaggcg ctggatgtga attaacacat
     6901 tcctcagctt atttataaaa ggattattcc catttgatat aatatcagtg ccaatttact
     6961 tttttcaatg gaatatatct cggctaaata acaataaaac ttatcgctaa tggattaaat
     7021 aaacaatttt ctaattcatt tcaccacctt tgatatagaa attgatggtg ttgtcgctgc
     7081 cattgcttat ggtgatgata aaatccacgt ttctggatcg ttgcgtagaa aatcatcact
     7141 accttgatat tttgatgcga tatgatcata ccgttccgta gtaccaattt caatttcagg
     7201 ctaaacccca acatt
//
LOCUS       DAZGBC010000205         7206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGBC010000205 DAZGBC010000000
VERSION     DAZGBC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.206"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2050
                     /locus_tag="ACHK1J_004477"
     CDS             <1..2050
                     /locus_tag="ACHK1J_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO7913658.1"
                     /translation="KSRKSVKSVEPDIADRANNWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGTGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDGTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDARPTLDDFKKTVGDYLAWEVSQLLKKQGENNFAGK
                     "
     gene            2119..2598
                     /locus_tag="ACHK1J_004478"
     CDS             2119..2598
                     /locus_tag="ACHK1J_004478"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7913659.1"
                     /translation="MSTGSLISSGRLKPGKIKRVSAKSDQNGIIGEFDTEFMDDARHY
                     ENFISVNFFGDAFYHPYCASVEMKVHVLTLKSSKSDFNAKRGLYVASMINKALKGRFN
                     YGQQLSSSKLRDGEYFISLPVKNNEIDWFFMDNYIEELEAAHIEELEAYLLVSGLAD"
     gene            2661..3182
                     /locus_tag="ACHK1J_004479"
     CDS             2661..3182
                     /locus_tag="ACHK1J_004479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7913660.1"
                     /translation="MWGTYNLKKLFGPAIRGKRLKNADRIPGSLPFVTAGEGNEGISA
                     FISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAVVHTEFFDRNAVIFITASIHKS
                     SCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFMETLILAVHKLVIKDVVQYTDKK
                     LVAYRQITSEKVV"
     gene            complement(3539..3757)
                     /locus_tag="ACHK1J_004480"
     CDS             complement(3539..3757)
                     /locus_tag="ACHK1J_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013575645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913661.1"
                     /translation="MLIYNVFGRHIGVKREGDHWLVFRADLTERKFSRLYDIVIPDDM
                     TEDEIPGWLSDIFHEAATERHPDVKRIR"
     gene            complement(3988..4085)
                     /locus_tag="ACHK1J_004481"
                     /pseudo
     CDS             complement(3988..4085)
                     /locus_tag="ACHK1J_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4617..6527
                     /locus_tag="ACHK1J_004482"
     CDS             4617..6527
                     /locus_tag="ACHK1J_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7913662.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
     gene            6667..6849
                     /locus_tag="ACHK1J_004483"
     CDS             6667..6849
                     /locus_tag="ACHK1J_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7913663.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            6875..7147
                     /locus_tag="ACHK1J_004484"
     CDS             6875..7147
                     /locus_tag="ACHK1J_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7913664.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT         2054 a         1467 c         1831 g         1854 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacaactg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcaca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggcag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat ggtacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcgttcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccactgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctagcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag atgctaggcc aacgctggat gatttcaaaa agacggtagg
     1981 tgattacctt gcatgggagg tctcgcaatt actgaaaaaa caaggtgaaa acaattttgc
     2041 gggaaagtaa attccccgct cagcgataaa ttaagaagcg ttgagtgggg agagttcagg
     2101 attggcgatt tgttctatgt gagcacaggc tcgcttatct cctcaggaag attaaaacca
     2161 ggcaaaatta aacgtgtttc agcaaagagc gatcaaaacg gtatcatcgg agagtttgat
     2221 accgagttta tggatgatgc aaggcattat gaaaatttta taagcgttaa tttttttgga
     2281 gatgcttttt atcatcctta ttgtgccagc gtagaaatga aggtgcatgt tcttacatta
     2341 aaatcaagta agtccgactt taatgcaaag cgcggactgt atgttgccag tatgattaat
     2401 aaagctttaa aggggcgttt taattatggt caacagttat cgagttccaa actcagagat
     2461 ggagaatatt tcatctcatt accagtgaaa aataatgaaa ttgactggtt ttttatggat
     2521 aattatatag aagagctgga agcggcccat atagaagagc tggaagctta cttgctggtt
     2581 agcggactcg cggactgata agctatgagc ttacaccgca ggaatataac gcgattgagc
     2641 tattaaatag caataatctt atgtggggca cttataacct caaaaagtta tttggccccg
     2701 caataagagg taagcgtctg aaaaacgcag atcgtattcc cggatccttg ccttttgtta
     2761 ctgcgggtga aggcaatgaa ggtatttctg catttatcag taatgatgtt gttgtattcc
     2821 ctgaaaatac cattacgata gatatgtttg gctccgcaaa atacagaaat tatgagtatg
     2881 gtgcagatga ccatgtggca gttgttcata cagaattctt tgacagaaat gccgttattt
     2941 tcataacagc atcaattcat aagtcttctt gtaccggaaa gttcaactac gggagaaatt
     3001 tctatgcaaa agatgccgac gagttaatgg tatctttgcc gtcaaaagat aataaccctg
     3061 attattcttt catggagacg ctaattttag cagtacataa actggtcatc aaagatgttg
     3121 tccagtacac tgataaaaag ctggtagcgt acaggcagat tacatctgag aaggtcgttt
     3181 agtgcaaatc cccacctgac agggtgggga atggtcaggt tatcgtgccc tgatgcagaa
     3241 aaacagttta caatcgtaaa aatcatcata tcatgacaaa agtggttaca ttgccacgca
     3301 gccgaacccg ccgatgcgcg ggttttttgt atccggaatc ctgtgagtta tacggaaagt
     3361 acacagaaag gaaggtgcga ccgcaattaa taacaaaatc ttaaaaattg catatggcac
     3421 tattagtttt ctaaatattg tatattttga gtattgcagg atatcccctg taacgaagtt
     3481 tgcgtaacag cattttactc tacgagtttg ccagcctccc ccagtgggct ggcttttttt
     3541 atctgatgcg cttcacatca gggtgacgtt ctgttgccgc ttcatggaaa atgtcgctga
     3601 gccagcccgg tatctcgtct tctgtcatat cgtctggaat gacaatatcg tataaccggg
     3661 agaattttcg ttctgtcagg tctgcccgga agaccagcca gtgatcacct tcacgtttga
     3721 caccaatatg tcgaccaaag acgttgtaga ttagcactat cgacctcttt caggaattgt
     3781 gtaactgaga tgacagattc agatttcgtc aataaatgca aaatgtcaat cctgagatca
     3841 tggtgagcta tctggcggtt ttttatatct gtttaacggg ggatgttaca tacctcacaa
     3901 ttcagtcagt tgaaggttgt ctgcccggat gagaatttgt tagaaaattt ctgcatggtg
     3961 aatccccctg agcggagggg catatcagcg acaggtggtt ggttatatcc cttatctggt
     4021 gcgggttcag gtgctgatac tgaactcacc gggaggcacc cggcaccatg caatgaacgg
     4081 tacatagcgc tactcttcag cccctctccg gaggggcttt ttaatgaggg gaagacactg
     4141 ggcagtgaaa tgttaaatca ctcacaattc aggtagttga cggttgtctg cttggctgag
     4201 aatttgtaaa aaaaatcctg catggtgaat ccccctgtgc ggaggggcga ctggtgaatg
     4261 gtatgatctc tttgatgatc gtaagcgaga atacgcgggt ttggtggcac caggccgaac
     4321 tcaccgggag gcacccggca ccatgcagta tacagagatt aggcatatac caaggcccct
     4381 catagcaggg gcctttttac atgcaaaaaa agccgccccg ggaagagcgg ttggcaagaa
     4441 gaaaagcaat atgaacaatt aattaacgat gggaataata ccttagagta atcaccttgc
     4501 gcaactgtaa gggcatattt catctttgcg ggctgttttt ccgggtgact tctgtgtttc
     4561 cggaggccag cctgtacctt ctctgactca gaacattatc ccggccggga ggattcatgg
     4621 catttaaaca ctatgacgtg gtcagggcgg cgtcgccgtc agaccttgcg gaacgaataa
     4681 ctcaaaaact gaaggaaggg tggcagcctt atggtagtgc gctgatttcg acagctggtt
     4741 atggtgcgga gttcatccag ccagttgtga gtgaggggag catctcatca ccagaggagc
     4801 caggcaaccg tccgacgacc tcagcgcctt ctgttgcgcc agaatattac tatgtgatcg
     4861 cgcttgctgg tcagtccaat ggtatgtcat acggtgaggg actgccatta ccggatacat
     4921 tcgacagccc tgatccacgt attaaacagt tagcgcgtcg cagtacggtg acaccgggag
     4981 gtgctgcatg tatctataac gatattatcc cggcagatca ctgtctccat gatgtggagg
     5041 atatgacagg gcttaatcat cccaaagcgg atttatcgaa aggtcagtac ggctgtgtgg
     5101 gacaggggct gcatatcgcc aaaaagcttc tgccatttat accggcgaat gcgggcattc
     5161 ttcttgttcc gtgctgccgt ggtggttcag ctttcaccac cggaaccgat ggcacataca
     5221 gtgacactac tggcgcctca gagagttcca cccgctgggg ggtgggcagg ccgctgtata
     5281 aggacctcat tggtcgtaca aaagccgcgc tggcgaagaa tccgaaaaat gtgctgcttg
     5341 ccgtggtatg gatgcaaggg gaatttgatt ttaacggaac gccagcgaat catgcagccc
     5401 gttttacaga agtggtggat caataccgtg cggaccttgc agatatggcg gggcagtgtg
     5461 tgggtggctc agctgacagt gttccctgga tttgtggaga cacaacatat ttctggaagc
     5521 agaagaacga aacggcgtac cagacggtgt acggtagtta caaaaataaa acggaaaaga
     5581 atatccattt cgtgccgttc atgaccgatg agaacggggt gaatgtgccg acgaacaaac
     5641 cggaagaaga cccggatatt ccggatatcg ggtattacgg ttcaaaatgg cgaacggaca
     5701 gaagcacctg gacatctcag gacagggcca gtcatttcag ttcatgggct cgccgtggga
     5761 ttatttccga ccgtctggca acggcgattc tgagctgcgc gggtaagtct tctgcgtttg
     5821 ttaatggtac tgccggggtg gttgttccag acagaccggt taccacctca gagtctgtaa
     5881 ttttttacga tgccaaaaaa gcttcagaca atcagctgaa accttatggc tgggacggta
     5941 tggatggcag acgcacactg gttgatgaca gcggcaataa agctctgcga attgagaaaa
     6001 ataacagcgc gaaatcctgg tcaatgtact gtgatattgc tgcagacaag gcaaaacttt
     6061 tactggaaaa aggcggggaa attgctgtcc ggtttaaaat ccccgaaaac gtcaatcttg
     6121 agacaaccag aaacaagtat gcctttggtt tgtactggcg aatagcggaa tggccgggtg
     6181 agggtggtga aggctatctg agttctttct ttgtccagac agataaagcc agtattgatg
     6241 ttgcatacca tcatacagtt aatcaacaaa aagaacttgg cacgtttggc gcattcgacc
     6301 atgactggca tacgcttgca tttaaattta agggcagtaa cagcattaat gttactccgg
     6361 tgcttgatgg tgtggatgga caggcgtttg acctggtgaa atgggccaat actgctaatg
     6421 gactcaacag gtttgtcatt acggatatta caggtagtgc agaaacctac cctgtactta
     6481 ttgatacggt ggaagttaaa gcaaacaaag ctggagcagc cgcataattg ctaaaaaaag
     6541 ccgccagcgg caggaatgga agctggcgga ggtaatccca atggagaatg taaagaaaag
     6601 atgctttcgt acattggttt tttaaatgaa aacagttctc attgtcaacc ataacggtaa
     6661 gaaattatga tatttattca tcaggtgatg ctgtacttct gtacggcggt ctgtgtgctg
     6721 tatcttcttt cgggtgggta cagggcagtg cgcgatttct ggcgcaggca gattgataaa
     6781 agggccgctg agaaaatcag cgccagtcag tcagccggaa gcaaacccga agagcccgtt
     6841 actccttaac aacccctttc agcgagaaaa tcccatgtca gaaattacat ccctggtcac
     6901 tgcagaggca gtgaaggaag tcctgcgctc tgaagaagtc cggagcgcac tgaaacagaa
     6961 acttcgccag aatcttgagg cgcgtcttga tgcagaagtt gatgccattc tggatgagct
     7021 gcttggtgta caggcagagc caccgactga agcgggagat accaccgcag agagcggtga
     7081 agttcagcct gaatcaccgg tcgccgatgc gactgaacct caacccgaat cggtcatgat
     7141 gctgtaacga ggagtcaggg ccatcagtaa acggctgctg gcctttttca tgttgtgagc
     7201 ttccgg
//
LOCUS       DAZGBC010000206         7144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGBC010000206 DAZGBC010000000
VERSION     DAZGBC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.207"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..752
                     /gene="csgD"
                     /locus_tag="ACHK1J_004485"
     CDS             102..752
                     /gene="csgD"
                     /locus_tag="ACHK1J_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO7913665.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQEHVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            757..1146
                     /gene="csgE"
                     /locus_tag="ACHK1J_004486"
     CDS             757..1146
                     /gene="csgE"
                     /locus_tag="ACHK1J_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO7913666.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1171..1587
                     /gene="csgF"
                     /locus_tag="ACHK1J_004487"
     CDS             1171..1587
                     /gene="csgF"
                     /locus_tag="ACHK1J_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO7913667.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1614..2447
                     /gene="csgG"
                     /locus_tag="ACHK1J_004488"
     CDS             1614..2447
                     /gene="csgG"
                     /locus_tag="ACHK1J_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO7913668.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2511..3002)
                     /gene="ycdZ"
                     /locus_tag="ACHK1J_004489"
     CDS             complement(2511..3002)
                     /gene="ycdZ"
                     /locus_tag="ACHK1J_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7913669.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALTPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3104..3658)
                     /gene="ycdY"
                     /locus_tag="ACHK1J_004490"
     CDS             complement(3104..3658)
                     /gene="ycdY"
                     /locus_tag="ACHK1J_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO7913670.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3682..4419)
                     /gene="ycdX"
                     /locus_tag="ACHK1J_004491"
     CDS             complement(3682..4419)
                     /gene="ycdX"
                     /locus_tag="ACHK1J_004491"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO7913671.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRALAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4474..5412)
                     /gene="ghrA"
                     /locus_tag="ACHK1J_004492"
     CDS             complement(4474..5412)
                     /gene="ghrA"
                     /locus_tag="ACHK1J_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO7913672.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5646..5733
                     /locus_tag="ACHK1J_004493"
     tRNA            5646..5733
                     /locus_tag="ACHK1J_004493"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5680..5682,aa:Ser,seq:gga)
     gene            complement(5883..6725)
                     /locus_tag="ACHK1J_004494"
     CDS             complement(5883..6725)
                     /locus_tag="ACHK1J_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7913673.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITCNLDREIWRDLMQCS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDQWGLHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSLQGKECYEDEMFIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6810..7007)
                     /locus_tag="ACHK1J_004495"
     CDS             complement(6810..7007)
                     /locus_tag="ACHK1J_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7913674.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(7019..>7144)
                     /locus_tag="ACHK1J_004496"
     CDS             complement(7019..>7144)
                     /locus_tag="ACHK1J_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7913675.1"
                     /translation="RLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPTFDW"
BASE COUNT         1882 a         1771 c         1655 g         1836 t
ORIGIN      
        1 attactacac acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca
       61 gctgtcaggt gtgcgatcaa taaaaaaagc ggggtttcat catgtttaat gaagtccata
      121 gtattcatgg tcatacatta ttgttgatca ctaaaccttc tttgcaggcg acggctctct
      181 tgcagcacct taaacaatcg ctggcaatta caggaaaatt acataatatt caacgttctc
      241 tggacgatat atcttcaggc tctattattc ttctggatat gatggaagcg gataaaaagc
      301 ttatccatta ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc
      361 taaatacgcc tgaagattac ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg
      421 ttttttatgc catggaggat caagaacatg ttgtcaatgg gttgcaaggc gtcctgcgcg
      481 gcgaatgcta ctttacgcaa aagcttgcca gctacctgat tacgcattcg ggtaactatc
      541 gttataacag cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataagc
      601 tgcgtatcgg cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg
      661 ttaaaacgca tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg
      721 tttcctgggc aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt
      781 gtggcggcag aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg
      841 ggattgctaa ctgaccatac tgtttcatct attggccatg atttttaccg agcctttagt
      901 gataaatggg aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga
      961 tggggaagtt ggatcactat aacggtcaat caggacgtta ttttccagac ttttttattt
     1021 ccgttgaaaa gagacttcga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca
     1081 ctaaatcgtc gccagataaa tcaggcgtta ttaagtacgg acgatttggc gcatgatgaa
     1141 ttctaaataa aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc
     1201 atgcttattt cgccattaag ttgggctgga accatgactt tccagttccg taatccaaac
     1261 tttggtggta acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct
     1321 tataaagatc cgagctataa cgatgactat ggtattgaaa caccctcagc gttagataac
     1381 tttactcagg ccatccagtc acaaattttg ggtgggctac tgtcgaatat taataccggc
     1441 aaaccgggcc gcatggtgac caacgattat attgtcgata ttgccaaccg cgatggtcaa
     1501 ttgcagttga acgtgacaga tcgtaaaacc ggacaaacct cgactatcca ggtttcgggt
     1561 ttacaaaata actcaaccga tttttaagcc ccagcttcat aaggaaaata atcatgcagc
     1621 gcttatttct tttggttgcc gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag
     1681 aagccgccag accgacatta atgcctcgag ctcagagcta caaagatttg acccatctgc
     1741 cagcgccgac gggtaagatc tttgtttcgg tatacaacat tcaggacgaa accgggcaat
     1801 ttaaacccta cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc acggcaatgc
     1861 tggtcacggc attgaaagat tctcgctggt ttataccgct ggagcgccag ggcttacaaa
     1921 acctgcttaa cgagcgcaag attattcgtg cggcacaaga aaacggcacg gttgccatta
     1981 ataaccgaat cccgctgcaa tctttaacgg cggcaaatat catggttgaa ggttcgatta
     2041 tcggttatga aagcaacgtc aaatctggcg gggttggggc aagatatttt gggatcggtg
     2101 ccgacacgca ataccagctc gatcagattg ccgtgaacct gcgcgtcgtc aatgtgagta
     2161 ccggtgagat cctctcctcg gtgaacacta gtaagacgat actttcctat gaagttcagg
     2221 ccggggtttt ccgctttatt gactaccagc gcttgctgga aggggaagtg ggttacacct
     2281 cgaacgaacc cgttatgctg tgcctgatgt cggctatcga aacaggggtc attttcctga
     2341 ttaatgatgg tattgaccgt ggtctgtggg atttgcagaa caaagcagaa cggcagaatg
     2401 acattctggt gaaataccgc catatgtcgg ttccaccgga atcctgacag ataaaaaagc
     2461 cggaattatc atccggcatt ttactgctta agcgaaagcg tgagtgcccc tcacgcttta
     2521 tttttgattt cctgctcacg gtgcgccgtc tttgcactac gcgccgccag ccacaggccg
     2581 ctgtttttca ttgcgtaacc aaatatcagc ccaagcgcca gcgaaggtaa caccagtttc
     2641 cagtcaccct gcccggcaaa cgtcgcgcat gcgcctataa acgtccccgg aacaaatgaa
     2701 agcagcagct gtttggcctg aatacacatc agaaacgcga caataccggt tatgacataa
     2761 ccgagaattt ccaaatgtgg cgtcagtgca ctaccgtaaa taatgaccat cgcccacacc
     2821 acgccactaa gcagggttgc agcggagatc gccagccctt tcagcccacc ttgcgggcag
     2881 gcaaagtagg ccgtacagcc caggaagccc gcccagctta gtaagcccag ggaaacagcc
     2941 acccatcccc agataccgga gagaatgcct gttgtgattg caatagagag aagtatgttc
     3001 atgccgcgca tgatagcaga aaacgcggcc atgaatgaga tcgagcacac attttatgca
     3061 acagcaaatt atctgttgca tttatgagtg acgcagatca catttactct tcagaatctt
     3121 cttccagctc gtcccacatt gcactaatgg catcgcgggt tagcggtgcc atggtgcgcc
     3181 agaaaggcgt ggttgcatgg gcctccactt tgccaaggaa cgcaccacac cagggtaaca
     3241 gatactcact gaacagtgtt tccagtgctt cgctctcatc ttccgttgac tgatcttcca
     3301 gccaggaagc cgcaagcagc aatgtgccga tgtgatcggc aggcgtatcc gctaatggca
     3361 tccctcgttc agaaagaaaa gcgcgcactt ccgcttccgt tgcgtcctca acccatgcgc
     3421 tacgatatgg cggcacggca cattcatcgc cgataaacaa cgcattgtaa tcggcagaga
     3481 cttgcgtcat atcacagctt ttctgcaaac gtgtcagcaa ctcatcctgc tccagtggcc
     3541 agttcgcagc cagtttcccc tcacgaatca gggtaaacag cggcaccagt aaaggatctt
     3601 gcggttggcg gtaatagagc gaacccagca cacgacagag gatagaaaac tcgttcatta
     3661 atatatttcc attactagta attaaaggtc tgcaaattcc gcaatcggtg ccataccgcg
     3721 agattcaagg aagttcagta agcggcgcgg agaaacattc aaaatgcgct ctggcggaaa
     3781 atctaccgcg tcgaggattt taagacactc ttcaaattcc cccatggtaa acgcggtgtg
     3841 agaatccgag cctaatgcca cccaacctcc agcatcacgt accgctgcgg ctagcgcacg
     3901 acagttgtct tcactgccct tacgtgagtg taaaaatgag gaattattga tttccagcgc
     3961 cacctgatgt ttcgcggctg cttcagcaac ggctttaaca tctatctcat atttcggatt
     4021 tccgggatgg cttattatat gcacattgcc gctggcgata gtggcgatca tcgcttgtgt
     4081 attggtcgct ttatcatgtg gcgcaaaaac cggctcatga aaaccggcaa taatcagatc
     4141 cagcgaatca aacattttgc cgctgcagtc aatttcacca tcaacatttt taatgttagc
     4201 ttcgatgccg cgcaggatcc ctaccccatc aaccactcgc ggccagatac gcatgttaat
     4261 gaagtgccag tgatgtggcg catcttccat atccgggcca tgatcggtga tcgcaaaaag
     4321 tttaatgccc ttttgtttgg cctgggcaat ataatcactc aatgtgctat atgcatgcgt
     4381 gctggcaacg gtatgcatat gaaggtcgac gggatacata actctctcct gtattcaatt
     4441 cccgccaagg atagcaggaa tcctgatgct ttattagtag ccgcgtgcgc ggtcgacttg
     4501 cccgcagacc ctctcccctt tttcgagctg ggcaatggtg cgagaaatgt actccacagc
     4561 ttcagcggga cgggtaatcg cggcgacatg tggtgttatc gtcacgcgtg gatgttgcca
     4621 gagcggactt tcaggcggta agggttcacg attaaaaaca tccagcattg cgcctttaac
     4681 tttcccgcta tccagcgccg cgagcaggtc atcttccaca acatgaacac cacgcgccag
     4741 gttgaggaga tacgcgccat ccggtaattt ttcgagtaat tgttgattaa taatgccgac
     4801 ggtttcaggg gtattcggta acaaattaat caatacccga cattggctca gaaatgcaga
     4861 cagttcttcc cgtccggcaa agctttgcac gccaggccac gatttacggg ttcgactcca
     4921 gcaacgcagc ggaaagcgcc aggtttgcag actctgagca actttactgc ccagtacgcc
     4981 tgcgcccaaa atgccgatgg taaaatcttc ccgatgatat tcaggcagcg gttgccaatg
     5041 cgaactattt tgctggatgc gataatcgtc aaaacgtcga aaccaatgca gcacctgact
     5101 gacagcatat tcctgcattt gctcgcccat accggtatct tccaggcgaa aaagtggaac
     5161 agaagggttc agcatttcag ggtgtgcctg tagcttgctc aaaatagaat caacaccggc
     5221 cccgagtgcg aacaccgctt taagatcgcg ccctgccagc atttcaacag gaggatgcca
     5281 gactaaagca taatcagcag agtcattatc tccgcttttc catgctctga ctcttgcctg
     5341 aggaatagct ttgcgcagtg cctcaatcca ccattgggta tcgaacgttg ggtgataaaa
     5401 gatgatatcc atactgactc ccgaaaagcg ttgtgcgaaa tttattcgca cttatcgtta
     5461 tgatctacaa agaccaccag cataacaaat ccgtggtcgg tggcaaaaaa agcagatttc
     5521 gcttattaaa accacatatt gattgaagtt tgaataaacg cgcgattttt taaaaaagtt
     5581 tgttgacctc aggtcatgat ttccctaaat tagcgcccgt tccagcaaga caggaacgac
     5641 aatttggtga ggtgtccgag tggctgaagg agcacgcctg gaaagtgtgt atacggcaac
     5701 gtatcggggg ttcgaatccc cccctcaccg ccagattaca agcgcatgtt attgtttaac
     5761 atgcgctttt ttatttatcc ttccagacag tatgccaccg ggtatgccat ctgaaaccgg
     5821 tttatatgcc ccacgatcat atcttatcgg acaatttccg atctttgagc atactggcaa
     5881 agctatactg ccagcatgtc aggatagtgt tttgcgataa tatcattcag tctgtcaacc
     5941 agttctggct tcctgaacgt gatgtgcgca gagcccttct taaagtattt aataatgaac
     6001 atctcatctt catagcattc ttttccctga agagaccgga tgtgatcgtc cagatgaata
     6061 gttatgtttt ccctgttgtc ggggatcggt ttgccgctga acagatgcag cattctttcc
     6121 aggtcggcaa gtctatctgc ctgttgcccg gtgataagat gcaatcccca ctggtcccac
     6181 ctcaccagat tgttgacgat aattttattg ccaaatttac aggggcaatt ggttttgtaa
     6241 ttccagctca accctctgaa gacgttgatc actcctcgct caaacacttc atccttattc
     6301 tggtgtagct gctcaaacgt actcagaata ttctcttcac tgatttccgg aaaattatcg
     6361 tactccagtg accggtacca tgtatcacgg gcctgtgcat ccattaagga aagcatcccg
     6421 gagcattgca tcaggtcacg ccagatttct cgatcgagat tgcaggtgat ggctttcatt
     6481 gccgtttcag gtttttccat cagccagcaa ccgcagcgaa aatcctgttt cattgcccag
     6541 tccagtgcgg ttttaccgcc aatactttct gtcattgctg atatttcagc cagttgccgg
     6601 ataagtgtgt cgatttgcag cagtgtactc tttcgtacag ccagaatgtg gcggatactg
     6661 gttgagcaga taacgtccgt atgcccggtg accagttccg gacgggagat tgtgatgtct
     6721 gacattatcg gtttcccttt gggtttataa acgaaaacgc cagccctgtt caggctgacg
     6781 tcagggtaat gaagtctggt gagtaagact caccggttct gttgcagaaa ctgcagttgg
     6841 cgcagggtac ggatatcttc cctggcctgt tcaatgcagg gcagcagtgc tgccgaatcc
     6901 gttttatcct catcgttgtc aaagataatt cccgattcgc agtcgatatt gtcctgcagc
     6961 catgcaatca gaatatccag cgctgtttcc gtggttaatg atttcatgtt gtttctggtt
     7021 accagtcgaa agtggggaaa cccggcagac atccggcact ggcatccaga tgaatgagac
     7081 tgacgccata acgtcggatg agtgtgacaa ccagacggcg gaacgttttt gacagcccca
     7141 ggcg
//
LOCUS       DAZGBC010000207         7060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGBC010000207 DAZGBC010000000
VERSION     DAZGBC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.208"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1034
                     /locus_tag="ACHK1J_004497"
     CDS             <1..1034
                     /locus_tag="ACHK1J_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO7913676.1"
                     /translation="KKSNQLDADISKLNSYKNTLQSKIGDVNKQKEAEEKARENAEVA
                     EHETLNEEKQAVVEAEKRLDEAKAELAKAESDVQSKQATVSRVAGELENAQKSVDVKV
                     TGFPGWRDVQKKLQRQLEAKQAEYSAVENELKNAVSFRDGKAAEVKEAEQKLKEAQDA
                     LEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQDLAEKSKGKKIQGVDEALAAFEKYK
                     NVLDKKFSKVDRDAIFNALESVTYDEWAKHLEKISKALKVTGYLSFGNDVWDGTRKGL
                     ETGDWRPLFVTLEKSAVDFGVAKIVALMFSFIAGAPLGFWGIAIITGIVSSYIGDDEL
                     NKLNELLGI"
     gene            complement(1031..1321)
                     /locus_tag="ACHK1J_004498"
     CDS             complement(1031..1321)
                     /locus_tag="ACHK1J_004498"
                     /inference="COORDINATES: protein motif:HMM:NF037571.4"
                     /note="Members of this family include Cki (colicin K
                     immunity protein) for the pore-forming toxin Cka (colicin
                     K activity protein), as well as immunity proteins from
                     other related colicin systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cki family colicin immunity protein"
                     /protein_id="HFO7913677.1"
                     /translation="MYLKYYLHNLPESLLPWLLYLTLNYNNDISLAFIFFASIHVLLY
                     PYSKLTIFSFIQKTTNIKKEPWYSYNLFYLLYLAMAIPVGLPSFIYYTLKRY"
     gene            1346..1477
                     /locus_tag="ACHK1J_004499"
     CDS             1346..1477
                     /locus_tag="ACHK1J_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO7913678.1"
                     /translation="MKIVLFLITILSLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(1688..1832)
                     /locus_tag="ACHK1J_004500"
                     /pseudo
     CDS             complement(1688..1832)
                     /locus_tag="ACHK1J_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            2206..2394
                     /locus_tag="ACHK1J_004501"
     CDS             2206..2394
                     /locus_tag="ACHK1J_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913679.1"
                     /translation="MDNGVTAFEKGESARRSRTTERSESVSEEAEEHRKRHFLLTHLC
                     GISHPTWRIFHTIYTDAT"
     gene            complement(2546..2737)
                     /locus_tag="ACHK1J_004502"
     CDS             complement(2546..2737)
                     /locus_tag="ACHK1J_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO7913680.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            2805..3128
                     /locus_tag="ACHK1J_004503"
     CDS             2805..3128
                     /locus_tag="ACHK1J_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO7913681.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            3337..3771
                     /locus_tag="ACHK1J_004504"
                     /pseudo
     CDS             3337..3771
                     /locus_tag="ACHK1J_004504"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3824..4366
                     /locus_tag="ACHK1J_004505"
     CDS             3824..4366
                     /locus_tag="ACHK1J_004505"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO7913682.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRD
                     AISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVT
                     GGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSG
                     VKGENNWTVEKKNAVRLVRE"
     gene            4373..4606
                     /locus_tag="ACHK1J_004506"
     CDS             4373..4606
                     /locus_tag="ACHK1J_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO7913683.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
     gene            complement(5089..5511)
                     /locus_tag="ACHK1J_004507"
     CDS             complement(5089..5511)
                     /locus_tag="ACHK1J_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO7913684.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQST
                     TTFAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
     gene            5633..5911
                     /locus_tag="ACHK1J_004508"
     CDS             5633..5911
                     /locus_tag="ACHK1J_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913685.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(5903..6193)
                     /locus_tag="ACHK1J_004509"
     CDS             complement(5903..6193)
                     /locus_tag="ACHK1J_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913686.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            6448..>7060
                     /gene="cka"
                     /locus_tag="ACHK1J_004510"
     CDS             6448..>7060
                     /gene="cka"
                     /locus_tag="ACHK1J_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFO7913687.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGH
                     GNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINE
                     KQKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDEL"
BASE COUNT         1879 a         1416 c         1982 g         1783 t
ORIGIN      
        1 aaaaaaaatc aaatcagctt gatgcagata ttagcaaact taattcttat aaaaatacat
       61 tgcaaagtaa aattggtgat gtgaataaac agaaggaagc tgaagaaaag gctcgtgaaa
      121 atgcagaggt cgctgaacat gaaactctta atgaagagaa acaggcggtg gtggaagcag
      181 agaagcgttt agatgaggcg aaagctgaac tggcgaaggc tgaaagtgat gtacagagta
      241 agcaagctac tgtttccaga gttgcaggag agcttgaaaa cgctcagaag agtgttgacg
      301 tgaaggtcac cggatttccg ggatggcgtg acgttcagaa aaaactacag cgtcagcttg
      361 aagctaagca ggctgagtac tccgcagttg aaaatgaact taagaatgct gttagtttca
      421 gggatggcaa ggctgctgag gtaaaagagg cagagcagaa attaaaagaa gctcaggatg
      481 ctcttgaaaa gagccagata aaagatgctg ttgatactat ggttgggttt tatcaatata
      541 taaccgaaca atatggagaa aaatacgcaa aaattgctca ggatttagcg gaaaagtcta
      601 aaggaaagaa aattcaaggt gttgatgaag ctctggcagc atttgaaaag tataaaaatg
      661 tactggataa gaaattcagt aaagttgatc gggatgctat ttttaatgcg ttagagtctg
      721 ttacttatga tgagtgggca aagcatctag aaaagatctc taaggctctt aaggttaccg
      781 gatatttgtc tttcgggaat gatgtatggg atggtacccg gaagggatta gaaacaggag
      841 actggaggcc tttatttgtc actctggaga agagtgcggt agatttcgga gtggcaaaaa
      901 ttgtggcatt aatgttcagt tttattgctg gtgctcctct tggtttctgg gggattgcta
      961 ttatcactgg tattgtttct tcttacatag gggatgatga gctgaacaaa cttaatgagt
     1021 tgttagggat ttaatatctc tttagagtgt aatatatgaa acttggcaat cctactggga
     1081 ttgccattgc caggtaaagt aaataaaaaa ggttgtatga gtaccaaggc tcttttttta
     1141 tgtttgtcgt cttttgtata aaagaaaata tcgttagttt tgaatatggg tatagtaata
     1201 catgtattga tgcaaaaaat ataaaagcta gagatatatc attgttatag ttaagtgtta
     1261 aatataataa ccatggcaga agtgattcag gtaaattatg taggtagtat tttagataca
     1321 taattttttc ttttgttgga ggtgtatgaa aatagtgctt tttctaatta caatattatc
     1381 cttgtcagga tgtcaagcaa actatattcg tgatgtacaa ggaggaaccg ttgcgccatc
     1441 ttcgtcttct gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg atctcttgag
     1501 atcctttttt tctgcgcgta atctgctgcc tgtaaacgaa aaaacccgcc ttgtggcggg
     1561 ttcttccgtc cgatcagaag tcttcgacct cttctaatcg gcggtggtaa ctggctatcg
     1621 cagagcacag gtacctaaat tgtccttcta gtgtagtcaa aatcagactg atacttcaag
     1681 aactctgtta ccaacttata aatctcgctc tgctaacccg gttaccaatg gctgctgcca
     1741 gtggcgttaa gtcgtgtctt ttccgggttg gacccaagag aacagttacc ggagaaggcg
     1801 cagcagtcgg gctgaacggg gggttcctgc atacagcccg gcccggagcg aactgcctaa
     1861 gtcgccccga gataccagaa gacgtgagct atgagaaagc gccacgtgtc ccgcaaggga
     1921 gaaaggcgga aaaggtatcc ggtaatcgat atggcgggaa cgtgggggcg caggagggag
     1981 cgattcaccg gaaacggtga tgatctttaa gccacgtcgg gtttcgccac cgctggcgga
     2041 tttcatggtt gagccgtaag gctcccacag atgcaccgaa aaagtgtctg ttttgtgaag
     2101 tccgtcaggg gggcggagcc tatggaaaaa gcgcctccgg cgcggccttt tccggggttt
     2161 ttgttttttg cgcacatgtt ctttcccggg ttatccccgg attctgtgga taacggggtc
     2221 accgcctttg agaaaggaga gtccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2281 gtgagcgagg aagcggaaga gcaccggaag cggcattttc tccttacgca tctgtgcggc
     2341 atttcacacc cgacatggcg catttttcat actatctaca ctgatgccac atgattaagc
     2401 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgctaa
     2461 aacccgctga cgcgccctga cgggcttgtc tgctcccggc atccggtcac aggcaagctg
     2521 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga
     2581 ggcagctgcg gtaaagctcg tcggcgtggt cgtgaagcgc ttcgcagatg tcagcctggt
     2641 cgtcggcgtc cagttcattc agtttttcca gcagggtcag cgtctggctt ctgatgaagc
     2701 gggccatgtt aagggcggtt ttttcctgtt tagtcacagt tctgtttctc catgtgtggg
     2761 gagtctgtcg gaggttttta atgataccgg tgaaacgcga aagaatgctc acgatacggg
     2821 tcacggatga ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat
     2881 ggatgcgtaa ggtctgcctg ggggcaccgc catcgaaaac gtcaggcctt ccgacactgg
     2941 caccgccgct gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag atagcccgga
     3001 agataaacag cggacagtgg tcggggcatg accgggttca tgtggtggct gcactgatgg
     3061 ccatcgggcg tgaactgagt gagctgaggg atgaggtccg taaacaggga gaacgggatg
     3121 atagttaagt ttcatgccag gggaaaaggc ggtggcagtg gtccggttga ttacctgctg
     3181 ggccgggagc gtaaccggga aggggcgaga gttctgcggg gtgctcccga agaggtgcgg
     3241 gaactcatcg atgccacgcc gtttgcgaaa aaatacacct cgggggttct gtcttttgca
     3301 gagcagaccc tgccaccggg agagcgtgaa agggtgatgg agagctttga gcgggtactg
     3361 atgcccggtc ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca ggacaagggg
     3421 cgtcttgagc tgaattttgt catcccgaat atggagctgg cgagcggaaa acggctccag
     3481 ccctattacg accgggcgga ccgtccgcga atcaatgcct ggcagacact ggtgaatcac
     3541 cattacgggc tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt caccccgaat
     3601 aatctgccga aagcgaaaca ggaggccgca gaagcgatta cgaggggact ggaggctctt
     3661 taccatgccg gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac agcggcaggt
     3721 tttgaggtgg tgaggacaac caggagcagt atcagtatcg ccgacccgga gggcgggcgt
     3781 aatctcaggc tgaaaggggc gctgtatgag caatctttta caaatgggga cagacttcga
     3841 gaagaaactg aaagagcgtg ccgtctctac agagaacatg ctgaacagcg agttcaggaa
     3901 gctcgaagcg tctgtagaca gggaactgaa cttaaacggg cagaaaatca gagacgctat
     3961 cagcgcacac acagcagcgg tgaaagaaca actggagacg ctgaacagtg ccgtgaacgc
     4021 gcagttcagc gccacagaga acgaactggc ccggcggcag gaagagctgc tctggaagct
     4081 gatcaaaggg cggatactgt acccgtcgct gacggcgctt tgcgtcacgg gcgggatttt
     4141 tctggcgtca tgggggctga ttcagtggca ggaaagccgt attgcggcga acatcctggc
     4201 tatcagggac caggaagaga cgctggcgaa actccgggaa aaaacatggg gtgtgacgta
     4261 tcacgagggc aggaacggga aatttctggt gctgccgtcc ggggtgaagg gggaaaacaa
     4321 ctggacggtg gagaagaaga acgcagtaag actggtcagg gagtaagtga gtatgacaga
     4381 gctggaaatg cacttactga acgccttaga gcaattgcag caggactata cgcagcggct
     4441 gaaagactgg gagagcgcct tcacggattt gcagcatatg ttcgagctga cacgacagga
     4501 gaacgggcaa ctgagggaac agtgcgacga cttgagtcag caggtgcaga gattagccgg
     4561 gcaggtggac cgcttgagtc ggttatttat cacgaacagc agataaggga tgaacaggaa
     4621 aggcaggcac gcctggaacg ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa
     4681 gtacgggtat ggcgtggccc gtcaatgggg atgtgacggt gagttgttcc gggaggttgc
     4741 cagatggtac cggaacgcgt taaaaacgca tccgggcgcg attctggcgt gtcgtttttg
     4801 ttgctgtgtt ttgcctcttt gccagatcgc gctcattcct cagcctcatc ccgcgatgtc
     4861 tgcgtatgtc atcaactgaa aagatggaaa aaaacagttt catgaggttt acgcaggcaa
     4921 aaccagtgtc attcacatgg ctgaatagct atgcatacgg ttaaaattta tcaggcgcga
     4981 tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcga
     5041 tgaacgcgtt tcagcggtgc gtacaattaa gggattatgg taaatccctt actgtctgcc
     5101 ctcgtatcca tcgagataaa ccgcagaaat ggtgtctgcc attagtttca tttttttcag
     5161 cgaaggcata tcgctgtttg tgtattttct gccagcaaag gttgttgtgc tctggagtga
     5221 gtgaaaaaat ttttctgagt ggaattcttt tgcacgttgc tgtgccgttt ctgctgatac
     5281 tccggctgct ttgtctttca tccccatatc ccggaactgc tgaaagaacg ggagcatcat
     5341 acgtatggtg tctgctttct gggtatagaa gttaggatca aatccatcat ctgaagcata
     5401 aacataatgt cgggtatgtc gttccaggct ggtctttggg gtgcatcctg taagagctat
     5461 tgttgctgtt atcagaggta tcagaagcag cctggtagcg gcagatctca taaacatctc
     5521 cttgcgtttt taatcttgat actcagggag ttatatctcc gaagagtatc tcttatcata
     5581 cgtcggttta ctcccaatgt gatttgatgg tcaatgagtc aggaataatc ccgtgaaaaa
     5641 atgtgtgcca gtcctgcttt ttagtgttct ttctgcttcg gttgccagtc atgcggacag
     5701 caatgttttt caacaaaagg ccgagaagac aaaaaacggc attgttgcgg atgttaatgc
     5761 cagacagaaa atgttcagta acagactgaa gatacaactt cagatcgatg agttgagagc
     5821 agagctggct ggcaccagag atgcccaaaa aagagaggat ttgcgtaaaa aactggagtc
     5881 tctggagcgg caaaagaaat ccttacgctg atgggggcgt attttccccg gtttcttcca
     5941 taaccttttt accgttcaga tatttacggg cgaggcgaat gcgtcttgcc tggtataccg
     6001 ctgagtaagc ctgggtggga ttcacaccat tactgatttc actgaccaca tgacgctgat
     6061 cagccagtgt cagcgtgtaa acctgtttct ggatgaggtc acgggctttg tcatactcat
     6121 atccggcagc aacaagggtg tcgagaagtc gtttgtctgt tttgttcagt ttatggcggg
     6181 gtgatttgct cacatcgttc tccgtttttt catgatccaa cagttttata tctcatcctg
     6241 atttgctccg gtgttctgtt tcgacgttct caatgcctgc ctccccatag tgtgatcctt
     6301 attgcaaaaa tgaaaatcca tgctcttgac acggacaatg ctgagtagta ggttttcact
     6361 gtacataaaa ccagtggtta tatgtacagt atattgtttt taatttattg tttttaattt
     6421 attgttttaa ttatcaaaga ggaatttatg gctaaagaac taagtggata tggcccaact
     6481 gctggtgagt ctatgggggg aacaggggcg aatctgaatc agcaaggtgg aaataataat
     6541 agtaatagtg gggttcactg gggtggtggc tctgggcatg gtaataatgg tggtcaggga
     6601 aattcaaatt catctggttc tacatcaact gtgatgaaaa ccggtgagtc atatctgact
     6661 ccgtggggtg atgttgtcat taataatgat ggccttccgg tgatgaatgg tattgttatg
     6721 acagaagaga attcaactct ggttgataat ccatttggag gggtctccag agttcttaat
     6781 tctttaatta gtgatatgcc atcacttttt gcagaaagct caggaaataa caacaataac
     6841 actgcttcag ttaatactgc accaacgaat gctcaggtca gtgatatgga taagagttcg
     6901 aaggtggtgg gtaatgtaat taatgaaaaa caaaagcaga agaataaaat tgctacacag
     6961 attagtgaaa aacagaagaa aatcgaggag atgaaaaaag tattcaagca tcattcttat
     7021 catggtatca ctgatcttga gagggatgtt gatgagttgc
//
LOCUS       DAZGBC010000208         7042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGBC010000208 DAZGBC010000000
VERSION     DAZGBC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.209"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(172..657)
                     /gene="sixA"
                     /locus_tag="ACHK1J_004511"
     CDS             complement(172..657)
                     /gene="sixA"
                     /locus_tag="ACHK1J_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO7913688.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVTS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(860..3004)
                     /gene="fadJ"
                     /locus_tag="ACHK1J_004512"
     CDS             complement(860..3004)
                     /gene="fadJ"
                     /locus_tag="ACHK1J_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO7913689.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCESLVEMGARGESFWKTTATDLQ"
     gene            complement(3004..4314)
                     /gene="fadI"
                     /locus_tag="ACHK1J_004513"
     CDS             complement(3004..4314)
                     /gene="fadI"
                     /locus_tag="ACHK1J_004513"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO7913690.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SNLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4495..4779)
                     /locus_tag="ACHK1J_004514"
     CDS             complement(4495..4779)
                     /locus_tag="ACHK1J_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO7913691.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5151..6491
                     /gene="fadL"
                     /locus_tag="ACHK1J_004515"
     CDS             5151..6491
                     /gene="fadL"
                     /locus_tag="ACHK1J_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO7913692.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDISGRSDLTGQRADADNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFNDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERTAGSLGPLLSQQLIAQGVPQSWTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSLAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYHDKNWTFRTGIAFDDSPVPADKRSISIPDQDRLWLSA
                     GATYAFNEDASIDVGASYMHGQNVKFTEGEGPAAYTFKSEGKAWLFGTNFNYAF"
     gene            6856..>7042
                     /locus_tag="ACHK1J_004516"
     CDS             6856..>7042
                     /locus_tag="ACHK1J_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913693.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEVNGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVND"
BASE COUNT         1817 a         1839 c         1639 g         1747 t
ORIGIN      
        1 attacaactt agtctcattt cgtcagaaat taatagggaa gcagaggaaa aagtgcgatg
       61 aattctgtat cgaagcgtat caactccgtg aaaggttttg agtgaatatc tactattgca
      121 aaaagacaaa tgcccgatgt aagctgtaca tcgggcattt tttcaaacga atcagatagc
      181 tttcgccatc ttcaaattac atgggctcat ctgccagttg aactcgcctt ttccgctttc
      241 atcaagcgtt acgctggcaa tggccgaggt ggtaaacata ggcggcgttt cgcccggaca
      301 taactcggcg accagatacc caactaacgg taagtgggag ataaccaaca ccgaagtaac
      361 accttcattg gtcaacgcct gcaaataggc actgaccaga ccaacatcgc cgcagggcgt
      421 taactccggc agaacttctg cactggaagg caggttcaga caatccccta cttcctccag
      481 tgtttgctcg gctcgcagga acgggctcac cagaacgcgt tcgatttcca ctttttgacc
      541 tttcagccag ttcgccatca ggcgagattc gtcacaacca ttagtagtca gaggacgaac
      601 cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc atgataaaaa cttgcatatt
      661 gcaccgcttt tgttaaccag attcgcccgt tttctttcat ctgaaaccag agaaagaaaa
      721 cggtggccgg cattgtgcct tatccattca ccgaatgaaa cgctgttttt tacctcaatg
      781 gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca atccgccatt cagcacggat
      841 tcatatagct ttgaccttct tattgcaggt cagttgcagt tgttttccaa aaactttccc
      901 cacgcgcacc catctcgact aaactctcgc aaggggtaaa acgtgaacca tactgcgtgg
      961 ccagtcgttg cattattgca accacttcgc ccgcgccgag agaatcgata tagcggaacg
     1021 gtccaccgag aaatggcgga aaaccaatgc caaataccgc accaatatcc ccgtcacgca
     1081 cgctacggat aacctgctca tcaacacaac gtactgcttc attcagcatc aacatcacac
     1141 accgttcagc aacctgcggt gcggagagtc gcccctgccc ttgtgcacca atgagcgggt
     1201 aaatggcagg atcgacctgt tttttgcttt tacgcccttt ctgaccataa agatagaaac
     1261 cccgaccatt ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca acatttgcag
     1321 gcgcgctaaa acgttctcca taagcggctt ccagtacagg aataatttta gtcccggtgt
     1381 cgattcctac ctcatccaaa agttggattg gacctaccgg aaaaccaaat ttcactagcg
     1441 cggcatcaat gtgctctacc cgttcacctt cggtcagcat gcggatagct tcattaatgt
     1501 aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc acgcacgaca atgggcgttt
     1561 tgccctgttt tttcgccagt tttaccgtag tggcgatggt ttgcgctgat gtacccgcat
     1621 gaggaataat ctccaccagc ggcatttttt ccaccggact gaagaaatgc aggccgataa
     1681 cttgctcagg tcgcgtggcg tgagcggcga tatcaccaat cggtaaagat gacgtgttcg
     1741 aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc cgccaccatc tgttgtttta
     1801 attcgagatt ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag ccgcgatagt
     1861 ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc gctggctttg agatgacgac
     1921 ggcgaacttt gccctccagc tgatcccaac tgtacttcag cgcatgattt atgccctgcg
     1981 ggttgatatc tttaattctg accggaagcc ccgctttaca agcagtgaca taagcaatac
     2041 cgccgcccat caagccacca cctaaaatcc ccacgctgtt taatggcgca ggcggcgcat
     2101 cactgccggg atctttcttc acttccgtac tggcaaaaaa gatactacgc aaagcttgcg
     2161 attgtggcgt catcgccagt tcgccaaacg cccgtgcttc agcgtcatag ccactgctgg
     2221 tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc ggataattgc
     2281 cttgagtttt gtgttctgtt ttcttgccga ccattttgaa cagcagcgca cgacctaacg
     2341 gccccgccag aatacgctcg cgtacaggta gagggcggga agatgggcga tccttctttg
     2401 ccagctcaac agcggcttcc agcagaatgg agtgcggaac aacgtcatcc accagcccca
     2461 gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag gatcatctct aatgctgtgc
     2521 tgacgcctat cagacgcggt aaacgctggg tgccgcctga accgggtaac aatccaagtt
     2581 gtacttcagg caaaccgagc accgttttag gatcgtcagt acaaacgcga ccgtggcacg
     2641 cgagcgccag ctccagccca ccgcccaggc aagcgccatg aatagccgcg ataaccggaa
     2701 tgggcaaagc atgaatctcc gccatcaact gttggccctg ccgcgccaga gcttccgctt
     2761 cttgcgccgt tttgcagttg ccgatcatgt tgatgtcagc tccggcgata aagttgtctg
     2821 gtttcgcgga aataaatacc acgccgcgca actctttgtt ttcgcggagt tgcttaataa
     2881 tggcgcgcac ctgcgaggca aattccgcct tcagggtatt cattttctcg cccggtacgt
     2941 cgatggtgat gatagcaatg ttgtccagac gaacattaag agtaaacgct gatgccattt
     3001 ccattattcc gcctccacaa ccattgccgc gccaagccca ccggcagcac aggcggtcac
     3061 taaaccaaat ccaccgccgc ggcggcgaag ttcatgcaat gtctgggtta tcattcgcgc
     3121 gccggtcgcc gcaaagggat gcccgtatgc aatcgagcca ccaagcacgt taaatttgct
     3181 atcgtccact tcaccggtgg catgcgcacg ccccagtacg tcgcgagcaa aacgttcgct
     3241 ccccagtagc tgaatattcg ccagtgtctg agctgcaaag gcttcgtgca tatcgatcaa
     3301 tgtcagattg ctcatcgtca aaccggcacg ctccagcgcc agcggtgttg accaggctgg
     3361 accgagcaac atgtcctgcc agacatcgat agcggtaaat gcgtagctgc gcagatatcc
     3421 cagcggtacc agccctaatt ctttcgcccg ggattcagtc atcaggatca ccgccgctgc
     3481 gccatcggtc agcggcgtac tgttcgccgc cgttaccgtt ccgtgtttac gatcaaacgc
     3541 cggacgcagc tttgcgtaat cagcaagcga ggaattaccg cgaatattgt tgtcttccgc
     3601 gagcagttgt ttataaggag ggataaaggc agtcatcacc tcttctttga gttttccgtc
     3661 tgaccatgcc tgagcggcac gctgatgcga acggtgcgct aatgcatctt gctgttctcg
     3721 ggtgatgccg taggttttcg ccatttgctc tgcagtgtcg cccatccgca agccggtaga
     3781 atattctgct accgcaggtg gtacgggcat taagtcgcgc aaacgcaggc gagagaagag
     3841 tttcagacgc tggctcattg tgcgggcttt gttgacatca accagcacgc gcgccagttt
     3901 tttactgacg ccaattggca ataccgaaga ggaatctgcc ccaccggcaa tccccgctcg
     3961 aatagttccc gccatcaggc tttctgcgac gtttgcaacc gcctggaaac tggtagcgca
     4021 agcgcggctg acgctgtaag catcggtatg cacattcatt ccggtaccga gaacaatttc
     4081 acgcgcaatg ttgggggctt caggcatttg tacgacctga ccaaagacca gttgttcaat
     4141 cacttcagcg gggatctcgc tgcgtgccag cagttcgcct accaccatct tccccaaatc
     4201 aacagcagga atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac gtaaaccgct
     4261 aacaatggcg atacgatcgc cctggcgggt aaccagcggt aaaacctgac ccataacact
     4321 cccctgtaaa aaaataaata aagtggtctg acctgatcat agtcttaacc atttttttac
     4381 atttagccaa gcggagaaaa gggaaagtgg gagctatgac acagagagaa aagaagaaga
     4441 gaaaagaaaa cgcccctgcc agcaattgac aggggcgtac gtacccgaga ggaattaacg
     4501 cagacccagc tggaagatca gcatttcagc ttcgcaggca aaagtgaaat cgatatccaa
     4561 gcgcacacca tcagactctt cagtgaaagt cggggagatt ttgcacggtt cggattccac
     4621 gctacgggct ttctcagtca gcgccgccag cgtttgttct gcttctgcgc ggtttgcaaa
     4681 cacgcggctg taagacgcgg tgcagtcgga gttgtccata atggtgccaa catccataca
     4741 gcagcaaacc ggggtttcat cagcactaca tttactcatc gttgatttcc tctgtatgtg
     4801 cacccaaggt gccagataaa cgttgtggat attttacgct tccggaaagt gctgctccag
     4861 ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca cttaaaaatg atctaaaaca
     4921 aaattcaccc gaatccatga gtgcgccacc tccaaatttt gccagctgga tcgcgtttct
     4981 tagatcatat ttgaaaaaag atagaaacat acttgcaaca ttccagctgg tccgacctat
     5041 actctcgcca ctggtctgat ttctaagatg tacctcagac cctacacttc gcgctcctgt
     5101 tacagcacgt aacatagttt gtataaaaat aaatcattga ggttatggtc atgagccaga
     5161 aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc acttatctcc acccaggcct
     5221 ggtcggcagg ctttcagtta aacgaatttt cttcctctgg cctgggccgg gcttattcag
     5281 gggaaggcgc aattgccgat gatgcaggta acgtcagccg taaccccgca ttgattacca
     5341 tgtttgaccg cccgacattt tctgcgggtg cggtttatat tgatccggat gtagatattt
     5401 ctgggcgttc cgatttaacg ggccagcgtg ctgatgcaga taatattgca ccaactgcgt
     5461 gggtgcctaa cctacatttc gtagcaccta ttaacgatca gtttggttgg ggggcttcta
     5521 ttacctcgaa ctatggtctg gccacggaat ttaatgataa ctacgctgct ggaatctatg
     5581 gcggtaaaac cgatctgcag acgatcaacc ttaaccttag cggagcctac cgccttaaca
     5641 atagctggag ttttggtctt ggttttgatg ctgtatacgc taaggcaaaa attgagcgta
     5701 ctgccggttc actcggtcca ttgctttccc aacaactgat agcgcaaggc gtgccacaaa
     5761 gttggacaaa cggcatcaat acaccggatt cgcaaattgc tcatctcaag ggtgatgaat
     5821 ggggattcgg ctggaacgcc ggtattcttt atgaactgga taaagataac cgttggggcc
     5881 taacctatcg ttcagaagtg aaaattgact ttgaaggaga ttacaaaagc tccatcaacc
     5941 caaatctgaa caacattttg ggtagcatgg gtttaccata tggcacaatg ggtgcaaccc
     6001 aaaatgggtc cttaacgctg aatttacctg agatgtggga aatttctggc tataaccgtg
     6061 tcgcaccaca atgggctatc cactatagtc tggcgtatac cagttggagt caattccagg
     6121 aactgaaagc gaaaggagac tctggtcaaa cgctgttcta taaagacgaa ggtttccggg
     6181 atgcttaccg tatcgcgtta ggtacaacct actaccacga taaaaactgg accttccgta
     6241 cgggtattgc attcgatgac agcccggttc cggcagataa acgttctatc tctatcccag
     6301 atcaggatcg tctgtggtta agtgcaggtg cgacttatgc atttaatgaa gatgcatcca
     6361 ttgacgttgg tgcatcttat atgcacggtc agaatgtgaa gtttactgaa ggtgaagggc
     6421 cagcagcata tacctttaaa tctgaaggta aagcctggct gttcggtact aactttaact
     6481 acgcgttctg ataacgcgtt cgcctggata aagtcacctg ctatgcaggt gactttaact
     6541 cccccacttc accgaaagta gtttcctcat tgcttgcctc ctgagttttg caaacagcct
     6601 gatggcagct tgccctttca aatcagtaag tggtatccac agacaaacct gaagggaaac
     6661 gcatattttc aggcgttctg ctcgtccttc tcaaagagtt tacttttctg aatttccagg
     6721 atactcaacc ccctggctat tgtgcgctca tacactcaaa ttaaagatag gttctaaata
     6781 aatgagcgtt ttttgatagt ctatttcatt aggtaatata tatttgtaac aaatcaatca
     6841 aaatggaata aaatcatgct accatctatt tcaatcaaca ataccagtgc agcttaccca
     6901 gaatccatca atgaaaataa caatgatgaa gttaatggat tagtacaaga gttcaaaaac
     6961 ctttttaatg gtaaggaagg aataagcacc tgtattaaac atctgcttga gcttataaaa
     7021 aacgccatac gagtaaacga ca
//
LOCUS       DAZGBC010000209         6984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGBC010000209 DAZGBC010000000
VERSION     DAZGBC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.210"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..1427
                     /gene="ulaA"
                     /locus_tag="ACHK1J_004517"
     CDS             30..1427
                     /gene="ulaA"
                     /locus_tag="ACHK1J_004517"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7913694.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1443..1748
                     /gene="ulaB"
                     /locus_tag="ACHK1J_004518"
     CDS             1443..1748
                     /gene="ulaB"
                     /locus_tag="ACHK1J_004518"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO7913695.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQYLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1758..2222
                     /gene="ulaC"
                     /locus_tag="ACHK1J_004519"
     CDS             1758..2222
                     /gene="ulaC"
                     /locus_tag="ACHK1J_004519"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO7913696.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2236..2886
                     /gene="ulaD"
                     /locus_tag="ACHK1J_004520"
     CDS             2236..2886
                     /gene="ulaD"
                     /locus_tag="ACHK1J_004520"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7913697.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2896..3750
                     /gene="ulaE"
                     /locus_tag="ACHK1J_004521"
     CDS             2896..3750
                     /gene="ulaE"
                     /locus_tag="ACHK1J_004521"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO7913698.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3750..4436
                     /gene="ulaF"
                     /locus_tag="ACHK1J_004522"
     CDS             3750..4436
                     /gene="ulaF"
                     /locus_tag="ACHK1J_004522"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO7913699.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4565..4840)
                     /gene="yjfY"
                     /locus_tag="ACHK1J_004523"
     CDS             complement(4565..4840)
                     /gene="yjfY"
                     /locus_tag="ACHK1J_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO7913700.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5167..5562
                     /gene="rpsF"
                     /locus_tag="ACHK1J_004524"
     CDS             5167..5562
                     /gene="rpsF"
                     /locus_tag="ACHK1J_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO7913701.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5569..5883
                     /gene="priB"
                     /locus_tag="ACHK1J_004525"
     CDS             5569..5883
                     /gene="priB"
                     /locus_tag="ACHK1J_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO7913702.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5888..6115
                     /gene="rpsR"
                     /locus_tag="ACHK1J_004526"
     CDS             5888..6115
                     /gene="rpsR"
                     /locus_tag="ACHK1J_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO7913703.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6157..6606
                     /gene="rplI"
                     /locus_tag="ACHK1J_004527"
     CDS             6157..6606
                     /gene="rplI"
                     /locus_tag="ACHK1J_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO7913704.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6677..>6984)
                     /locus_tag="ACHK1J_004528"
     CDS             complement(6677..>6984)
                     /locus_tag="ACHK1J_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO7913705.1"
                     /translation="NCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHD
                     LKIGLRSLSSSAAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1654 a         1741 c         1938 g         1651 t
ORIGIN      
        1 cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc tttaccgtgt
       61 tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc tgtctgggct
      121 acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa accataattg
      181 gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa ccggtggtgg
      241 cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc agatacttac gcttcgatga
      301 tggcaaccat cgaccgcatg ggcgatgcct atagctgggt gggttacgcc gtattgttag
      361 cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggcatt cgcacaatca
      421 tgttgaccgg ccacatcatg ttccagcagg ccgggttgat tgccgttacg ctgtttatct
      481 tcggctactc catgtggacc accattatct gtaccgcgat tctggtttcg ctctactggg
      541 gcatcacttc caacatgatg tacaagccga ctcaggaagt gacggatggc tgtggtttct
      601 ccatcggtca ccagcagcag tttgcatcat ggattgccta taaagtcgca ccgttcctcg
      661 gcaaaaaaga ggagagcgtt gaagacctca aattgccggg ctggctgaac attttccacg
      721 acaacatcgt ctccacggcg attgtgatga ccatcttctt tggcgccatt ctgctctcct
      781 tcggtatcga caccgtgcag gcgatggcgg gcaaagtgca ctggacggtc tacatcctgc
      841 aaaccggttt ctccttcgcg gtggcgatct tcatcatcac tcagggcgtg cgcatgtttg
      901 tggcggaact ctctgaagca tttaacggta tctcccagcg cctgatccca ggtgcggttc
      961 tggcgattga ctgtgcggct atctatagct tcgcgccgaa cgccgtggtc tggggcttta
     1021 tgtggggcac catcggtcag ctgattgcag ttggcatcct ggtcgcctgc ggctcttcca
     1081 ttctgattat tctaggcttt atcccgatgt tcttctccaa cgccaccatc ggcgtgttcg
     1141 ctaaccactt cggcggctgg cgtgcagcgc tgaagatttg tctggtgatg ggaatgatcg
     1201 aaatctttgg ttgcgtctgg gcggtgaaac tcaccggtat gagtgcctgg atgggcatgg
     1261 cggactggtc gattctggca ccgccgatga tgcaaggctt cttctccatc ggtatcgcct
     1321 ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc cgcgcactgc
     1381 gcgcagaaga agatgcagaa aaacaactgg cagaacagtc tgcttaataa ggagttttga
     1441 ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc atgatcatga
     1501 aaatgaaagt ggaccagtat ttaacccaat caaacattga ccacacggta aacagctgcg
     1561 cggttggcga gtacaaaagc gagttgagcg gcgcggatat catcatcgct tctacgcaca
     1621 tcgcgggcga aatcaccgtt accggcaaca aatatgtggt tggcgtgcgc aacatgctct
     1681 ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agagcatttc ccgcaggacg
     1741 tgaagtaagg atacgccatg aaactacgtg attcgctggc ggagaataaa tctatccgcc
     1801 tgcaggctga agcagagaca tggcaggacg cagtaaaaat cggcgttgac ctgctggtgg
     1861 cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatggcgtg gaacagtttg
     1921 gcccttattt cgttatcgct ccggggctgg cgatgccgca cgggcggccg gaagagggtg
     1981 ttaagaaaac aggtttctct ctggtaacgc tgaaaaagcc gctggaattt aatcacgacg
     2041 acaacgatcc ggtggatatc ctcatcacca tggcggcggt cgatgccaat actcaccagg
     2101 aagtgggcat catgcagatc gtcaacctgt ttgaagatga agagaatttt gaccgtttac
     2161 gcgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac gcggcagctt
     2221 aagaaggaat tgaacatgtc attaccgatg ttgcaagtcg cgctggacaa ccagactatg
     2281 gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat cgaagtgggt
     2341 accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc gctttacccg
     2401 cacaaaatcg tactggcaga cgccaaaatt gccgatgcgg gcaaaatcct ttcgcgtatg
     2461 tgcttcgaag ccaacgctga ctgggtgacg gtaatttgct gtgcggatat caacaccgcc
     2521 aaaggcgcgc tggacgtggc aaaagagttt aacggcgacg tgcagatcga actgaccggt
     2581 tactggacct gggaacaggc gcaacagtgg cgcgatgcag gcattcagca ggtggtttat
     2641 caccgcagcc gtgacgcgca ggccgcaggc gtggcgtggg gcgaagcgga catcaccgcg
     2701 atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggaggcct ggcgctggaa
     2761 gatctgccgc tgttcaaggg tattccgatt cacgtcttta tcgcgggccg tagtatccgt
     2821 gatgccgctt ctccggtgga agccgcacgt cagttcaaac gttccatcgc tgaactgtgg
     2881 ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga aaaagcgctc
     2941 cccgccgggg agtgctggct ggaacgcctg caactggcaa aaacgttggg cttcgatttt
     3001 gtcgagatgt cggtagatga aacggacgag cgcctgtcgc gcctcgactg gagccgcgag
     3061 cagcgtctgg cgctggtcaa tgcgattgtt gaaaccggcg tgcgcgtgcc gtccatgtgc
     3121 ctttctgctc atcgtcgttt cccgctgggc agtgaagatg acgcggtgcg ggcgcagggg
     3181 ctggagatta tgcgtaaagc tatccagttc gcccaggatg tcggtattcg cgtgatccag
     3241 ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg tcgtttccgt
     3301 gacggcctga aagagagcgt tgagatggca agccgcgcgc aggtgacgct ggcgatggag
     3361 atcatggatt atccgttgat gaactccatc agcaaggcgc tgggctacgc gcactatctc
     3421 aacaatccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg ggataacgac
     3481 gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt gaaagacacc
     3541 aaaccgggcg tcttcaaaaa cgtgccgttt ggcgaaggtg tagtggattt cgaacgttgt
     3601 ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat gtggagcgaa
     3661 acggcggaag acccggcggc agaagtggcg aaagcgcgtg attgggtgaa agcgcgcatg
     3721 gcgaaagcgg gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtatttgaag
     3781 ccaacatgga gctgccgcgc tacgggctgg tgacctttac ctggggcaac gtcagcgcta
     3841 tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga
     3901 aagcggccga tatggtggtg gttgatatga gcggcaaggt ggtggaaggg gagtatcgcc
     3961 catcttccga cactgcgacg catctcgaac tctaccgtcg ttacccgtcg cttggcggca
     4021 ttgtccatac ccactccact catgccaccg catgggcgca ggcggggctg gcgatcccgg
     4081 cgttaggcac cacgcacgct gactacttct ttggcgacat tccgtgtacg cgcgggttaa
     4141 gcgaagaaga ggtgcagggc gagtatgaac tgaacaccgg caaagtgatt atcgaaacgc
     4201 tgggcaacgc cgagccgctg catacgccgg gaattgtggt gtatcagcac ggaccgttcg
     4261 cctgggggaa agatgcccac gatgcggtgc ataacgcggt ggtgatggaa gaagtggcga
     4321 aaatggcgtg gattgcgcgc ggcattaacc cacaactgaa tcacatcgac agcttcctga
     4381 tgaataaaca cttcatgcgt aaacacggtc ctaacgctta ttacgggcag aagtagaaca
     4441 cgcgctgcgg aaatttcctt cctcgggaga taactggtct aattccgcag ccgtttttca
     4501 aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct
     4561 ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca
     4621 tcagaattac gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt aatgcctgac
     4681 gtgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat
     4741 cgtccatatt tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta
     4801 gcaaaagcac agccagaagg gctaaaacac gactgaacat agatacctcc tcgacggctg
     4861 actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag
     4921 ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ccatgatgaa gtgtgatgaa
     4981 cttcaaatca gcgtgttagt ccactttggt gaaagatgag atttatttgt cggctggcct
     5041 tgagtttagc ggggtatgtc agtactataa cgcgtcattt ttcagccgac ctttaacacg
     5101 ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc
     5161 aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt
     5221 ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt
     5281 ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca caaagctcac
     5341 tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga aactaccttc
     5401 cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa
     5461 gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac
     5521 gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt
     5581 ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt
     5641 cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ctttcaccgg
     5701 caggcatggt gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac
     5761 agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag
     5821 aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga ttctggagac
     5881 tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt
     5941 tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa
     6001 gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc
     6061 tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt
     6121 cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa
     6181 gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac
     6241 ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa
     6301 gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagcagc taatgctcgc
     6361 gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt
     6421 aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt
     6481 gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac
     6541 gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct
     6601 gaataattcg ttattcaacg agacgtaaaa acgccgacca ttggtcggcg ttttgctttc
     6661 tatttttcgt caggtattag tttcgcaagt agatccattc accattctct tgtgtccata
     6721 agtcaggatg gtttgctggc gttagataca tacgagcgca actgatgtct cgctcctgcg
     6781 gcactcgctc gaaacccaaa cccgagtaga ttgcggctga acttgataag gaacgcagac
     6841 caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc
     6901 cgagggcatt agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg
     6961 gtagttcaga tgaccagcaa ttag
//
LOCUS       DAZGBC010000210         6887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGBC010000210 DAZGBC010000000
VERSION     DAZGBC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.211"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..560)
                     /locus_tag="ACHK1J_004529"
     CDS             complement(<1..560)
                     /locus_tag="ACHK1J_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFO7913706.1"
                     /translation="MHSENIAAYIGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KRYAGKHWTGKHRTWLANQSFSQPSQ"
     gene            621..809
                     /locus_tag="ACHK1J_004530"
                     /pseudo
     CDS             621..809
                     /locus_tag="ACHK1J_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1177..2433)
                     /locus_tag="ACHK1J_004531"
     CDS             complement(1177..2433)
                     /locus_tag="ACHK1J_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7913707.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLSDFANIFQYAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     NIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(2446..2733)
                     /locus_tag="ACHK1J_004532"
     CDS             complement(2446..2733)
                     /locus_tag="ACHK1J_004532"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7913708.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            complement(2749..3192)
                     /locus_tag="ACHK1J_004533"
     CDS             complement(2749..3192)
                     /locus_tag="ACHK1J_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7913709.1"
                     /translation="MLKNLLNTKVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            3463..4494
                     /locus_tag="ACHK1J_004534"
     CDS             3463..4494
                     /locus_tag="ACHK1J_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7913710.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKTSSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHMLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQ
                     NSDLVITMPGAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDSCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            4697..5377
                     /locus_tag="ACHK1J_004535"
                     /pseudo
     CDS             4697..5377
                     /locus_tag="ACHK1J_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5394..5702
                     /locus_tag="ACHK1J_004536"
                     /pseudo
     CDS             5394..5702
                     /locus_tag="ACHK1J_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(5805..6887)
                     /locus_tag="ACHK1J_004537"
                     /pseudo
     CDS             complement(5805..6887)
                     /locus_tag="ACHK1J_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1898 a         1672 c         1582 g         1735 t
ORIGIN      
        1 tgctgagatg gctgactaaa tgattgatta gctaaccagg ttcgatgttt gccggtccag
       61 tgcttccctg cataacgttt gtctgttcgc aatagcatgc tcagaattcg ctgtcgggca
      121 actctgctgt ctcgtttagc tgctgcacgg gctctgatta gatcccgcat agcttcatga
      181 gtcaggtcag ggatccagac aggggtaagt tcaccagccc gcagtaacct ggcaagagaa
      241 attgcgtccc gatgatcatt tttgattctg tctgtcggtg attttggtat ccgggaaggg
      301 gcaataacct ggcattttat attcatcgct gtgagttgat gatatagccc aaaaccacag
      361 gggccagcct catagcagga aagaatgttt ccgtaaagtc cctgcaattt ctgaaataat
      421 cgacgtacag cctgggcttc gttgttaatc gtaccataat aacgaacttc gccaagccgt
      481 tcaggtgcag caatagcaac cgccaacgtt tctttatgta catccagccc gatgtaagct
      541 gcaatatttt ccgaatgcat aatgacctcc tctggaatat cccaccaatg ctatagtgag
      601 tatataaagg tcataccatc tttatgttct ttggcagcgg tcacggcggc gagcgtggag
      661 cactgttgta cgggctgatc ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc
      721 tgcgccatat cctgagcgta ctgccggaat ggccttccaa ccgagttgat gaactcttgc
      781 catggaacgt agtactcacc aataaataag cgtcaatacg gtgctccgtt gacgcttacg
      841 atcaaagggc gtatatcacg gcgtcttccg gtaaggaact cttctggcat tggcgttgaa
      901 ttattacctg tggagatgac gctggatgag cggccttacc ccgcttttta cgcgaggtga
      961 ttatgaagat caatgactag ccgtcgaacc gtgtgcatga actgctgcac tgaaacttct
     1021 cagctgtaaa ataatcctta cgctacgtac ttcccggagc gcttacgatt aatcggatgc
     1081 agtcctgact ggaagaaaga aaaatgggaa aaaataagag gaattaatat cctccacgtt
     1141 cctgaaatcc tggaggatat taatgtcatc aaaatattag cgaacgataa tccctaacaa
     1201 aatacccagt gcgccaaagt cagcatcact gaaggtggtg ttagcaaaac caatgttacc
     1261 cagcacaggc aataagaata ctggcaggaa ggtaataagc aagccctgag cgaaggcacc
     1321 caaaatcgca ccacgacgtc cacccgttgc attaccgaat acccctgcag ctgcacctac
     1381 gaagaagtgc ggcaccacac caggaataat cacgatcatg ttcagcaaat acaacgtgaa
     1441 cataccgatt aaacctgcag caaagctact caggaaacca accagtacag cgttaggtgc
     1501 gtaagggaag acgaccgggc aatcgagagc aggtctggca ttcggaacca gcttgtctga
     1561 aatacctttg aatgccggga caatctccgc aatcaccatg cgcacaccct gcagaatgat
     1621 gtacacgcca gctgcgaagg taatggattg catgatagag aacatgaacc agtttttacc
     1681 accgctaagc tctttcaccg catctgcacc cgcaaacagg cacgtcacca ggaaaattat
     1741 gctcatggta aaagagatag caaccggcgt gtcgcgcaag aacagcagac ttttcggcac
     1801 attcatgtct tcagtcgagt gctctttatt accaaacttg ctgccgatga aaccggccaa
     1861 tacataagat aatgttgaga agtggccgat ggcaacgtca tcagaaccgg tcactttctt
     1921 catgtacgga tgcgcaatag ccgggaaaaa gaccattgcc acgccaacaa ccaaagagcc
     1981 aacggctata agcgtaatgc cggtcatgcc tgctgttgcc agaattaccg ctaccatcat
     2041 cgacataaac aacgtgtgat gaccggtcag gaagataaat ttccacggtg tgaaacgggc
     2101 aatcataatg ttgataacca tcgcaaagaa cataatcatc gccatttctt tcccgaagct
     2161 tttctgcgct acggaaacaa tggcttcatt gttcggcact acaccctgaa taccaaaggc
     2221 atactggaaa atgtttgcga agtcactcag tgacgacaca actaaacccg cccctgcgcc
     2281 taaaatcaca aaccccataa tggtcttgac ggtaccttta atgcattcgg ttaccggttt
     2341 tttctgtgca atcaggccaa tcagggcgat taaaccgacc agcactgcgg gttcggaaag
     2401 tacatcgctc atcaaaaaac gaaagaaatc catatcgccc ccagcttaca gtgcgcctaa
     2461 ttcggttaat gccacggata aacgttctct catggctttc ttatcaatca tattttccag
     2521 tgcgacgatt ttgcctgcga cagactgcgc gactaactgc tcggcaatgt ctttggtgcc
     2581 gacatagata tcactcggcg tccctttcgc ggagcccagg tcgacatgat caacatcagc
     2641 gctgacggac aaatccttca aaatgctttt aatgctcatt tccatcatca ggctggtgcc
     2701 taagccgtta ccgcaaacca ctgtaatttt cataaatgtt cctcaggatt aatagcgcgc
     2761 aataacagac aaaatgtcct gcgtcgtttt ggccgtcaaa atagcctgta tgtctttttc
     2821 gttatcgaat aacttggcga gttgagaaat cgcttcaatg tggctggtac tgtcggttgc
     2881 agccagcaca atcagtaatt tcactggatc gttttcatcg gcatcaaaat tcacacctga
     2941 agggatcagg gtcagtgcta atgacagctt gttagccccc tcttccgggc gcgcatgagg
     3001 catcgcaata ccagggccaa ccacatagta cggaccgata gtgtcgtgag agtgatagat
     3061 cgcatcaaca taacgtggtt cgatagaacc gttttcaatc aacggtcggc atgaaatcgc
     3121 cacggcttcc cgccagtctt tgacctgttc cacgacctga acgacctttg tatttaatag
     3181 attttttaac atgcgactga taactcccga tgataattaa caggagagta gatattttac
     3241 gcattaatta atgtgatgat tgtcgcattt gatagcgcta tctgataacg ctaccgatct
     3301 ttgtgatagc gctatcattt ctctgccgcc atgctttggc tatattcggt tcctgcatat
     3361 acaatgcaac aggatatttg ctaccgtggg caaaaactgg aaagagtaat ggtaaaataa
     3421 aagtaatgaa aacagctggt tgtaattatc catggagtcg aaatggaaaa aacacgtaag
     3481 cgccgtggta ccggacgggt gacgctgcag gaggtcgcta actttgcagg tgtcggaacc
     3541 atgaccgttt cccgcgcgct gagaacaccg gaacaggttt ctgacaagct gagggaaaag
     3601 atagaacagg ccgtagaaga gttgggttat attcccaaca gaactgctgg cgcccttgca
     3661 tctggtcaca gtcatacagt cgccgtactg gttccttccc tgacagataa aaccagctcc
     3721 cgctttatgc agtcgttgca gcaggtgctt aataaaaatg aattccagct actgcttggg
     3781 tgtcatgagt ataatcagct caaagaagct gaaatcctga tgacgcttct tcaaggtaac
     3841 ccggcggcgt tagttatttt tggttctcag ttggctgata aaactcacca gatactcgag
     3901 aaaacaaata tacctaccat taacgttgtc ggttcccctt tcagtccggc tcaaattacc
     3961 atcgaaacag cattttttga agcatcacat aaactgaccg aacatatgct ggaacagggt
     4021 tataagaata tcggatttat cggtgcgcat atggacaacc gcctgcaacg tcagcaactg
     4081 aacggctggc ataaagcaat gctgagctat taccagaact ctgatcttgt tatcaccatg
     4141 ccgggtgccg caagtttgca attggggcgt tacgcattaa acgagatatt actgcgacaa
     4201 ccagagctgg atgctgtgat ttgcagccac gaagagattg cgctgggtat catgtttgag
     4261 tgccagcggc gtttgttaaa aatccctgga aatatcgctg tagcttgtct tgacggttca
     4321 gacagttgcg atcaaacaca cccaaccttg acatccatcc gtattgacta caaaaaaatg
     4381 gggacagaga cgggtaagct gctgattgga ctattgaata ataatcatga tgaatcagaa
     4441 gagtcgcgta tcgtgcaatt caactaccag atagaattac gtcagagtac atgagctcaa
     4501 aaggggatta ttgcaaagtt agctattgtg gtagggatag ccgtttccgg ctattccccg
     4561 cgcagagccg gatgtgtgct gctaacgcat cctgtcaacg acggatgaaa agttatccac
     4621 ttatatctcc accaacggcc caatattgta ccgttcgtga cacatccatt tctacttcag
     4681 gtatttggcg tagtagcggg tgctaaaaat ttgccgcaaa accgcaatat cagcccgcag
     4741 aggcgctctc cagattatcc attcctatat tatctctgca ccggatgaat tactcgaaca
     4801 actcagaaat atgacacata tgcaacttat caggactctg ggatcctgga ggcctgatgc
     4861 cagtgaatac agtaatgtta ccaacgttta tcgcattaca ttgaaatccc ttgttcgacg
     4921 ctatttcgag tttcatgatg aaatcaccaa cttggatgtc atgattgcgg caattgttga
     4981 tgaactggca cctgaactga ttaaacgtaa tgctgttgga tacgaaagcg ctttgcagtt
     5041 gttgatctct gccggagaca atccctaacg gttaagaacc gaatcaggtt ttgcggcgtt
     5101 gtgcggtgtc agccagtttc ccctatcttc tggaaaaacg aaccattacc gacttaatcg
     5161 gggtggggag cgtgttgcaa atagtgcact ttacatcatc actataggat gtttacaaac
     5221 tgacgataaa acaaaggaat atgtctccag gcgagtagcc gaaggacata caaaaatgga
     5281 agcaatacgc tgtctgaagc gctatatctc acgcgaagtt tatacattac tacgaaatca
     5341 aaacaggcat gttaactgca tcccgataac gtcttgactc ttagaagggc gtccaaggca
     5401 gtcattattc gagcaggcag ttccggcagt tattgtgtcg ataccagatc aggcagagta
     5461 tgagccggcg cggaaaatgc tgggataaca gctcaatgga acgcttcttc aggagtctga
     5521 agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct cactccataa
     5581 cggacgatat cgttggatat tacagcgcac taagaccgca cgaatataac agtgggttat
     5641 ccccaaacga atcggaaaat cgatactgga aaaactctaa ctcggtggcc agtttttgtt
     5701 gaccacttca gccatcgcca gacgctaaca tgagttactc ttgttctgag ctacgtcacc
     5761 ttcagggaac tggttatcct cattctttat ttgtgacatt gtggttattg tgcgacaaac
     5821 gtggctatcc cgccccatat cccagataaa accacctgct cacgtgcgac agcgacaatt
     5881 gtaatattga cattctttcc tttggcctga agttttcgag acctcttgca aagcctgagt
     5941 tgagcatccc atgctctgtc aatgacgggg cggggtaaat tttcctgatt ttttgtattg
     6001 ccggactgat gcgtgtcgga tgccggtaac tccacgctgc ctcaaccaat agctttctgg
     6061 catagctgtt tccggctttg gtaatgctgc cctgatgtct gcttccaccc gatgaatatt
     6121 ctgatggcgt gataccaaac catgacatca gttgtcgtgg atgttcaaaa cgggtcagat
     6181 cacccagttc agaaattaat ccaaccgcag taataaactg aatccctcgc atggcttgct
     6241 gcgcctctac taccggatac agcctccatt cggtaacggc ctccttcggg cggattccag
     6301 ccgttcacat tgtgcctgcc ggtcttctat tgtacaacgg tgttcgtcaa aagctaattg
     6361 tcgccaggga ctttcgaatg agtatttgct gagccagtgt cggtgtgcag ggccccaatc
     6421 tgctcgcccg acataatgga ccccatgaac cagaagaaac gatttcagac gttgccttgc
     6481 atgccgtaaa tcatcgcggg cagatgccca tgcccgggcc aaatcccgga atgcctcatc
     6541 ttcaacgccg gatacgtaga cagcagaaag atctcctgca cgcagtgaac gcacaagtct
     6601 gatggcatca cggtgtcggt tttaccccgc tcccccggtt ttttcgggat aagtgagggg
     6661 gcgcagacca tgcagtcaaa accggatttc accagccgac gataaagtcc ataaccacag
     6721 ggtcccgctt tataaacgat actgacctgc gatgactttg acctgatacg ttgataaaga
     6781 ttatgaatgt cagtaggtga tgtgctaatt ttacccatca gttcaacagg ttctgaattg
     6841 atagcatagg agacagtaat cgattctttg tgaacatcta attcgac
//
LOCUS       DAZGBC010000211         6874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGBC010000211 DAZGBC010000000
VERSION     DAZGBC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.212"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(197..469)
                     /gene="rcnR"
                     /locus_tag="ACHK1J_004538"
     CDS             complement(197..469)
                     /gene="rcnR"
                     /locus_tag="ACHK1J_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO7913711.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            590..1414
                     /gene="rcnA"
                     /locus_tag="ACHK1J_004539"
     CDS             590..1414
                     /gene="rcnA"
                     /locus_tag="ACHK1J_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO7913712.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVRAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1633..1971
                     /gene="rcnB"
                     /locus_tag="ACHK1J_004540"
     CDS             1633..1971
                     /gene="rcnB"
                     /locus_tag="ACHK1J_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO7913713.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(2053..3087)
                     /locus_tag="ACHK1J_004541"
     CDS             complement(2053..3087)
                     /locus_tag="ACHK1J_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO7913714.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYGELSAQHNLVVQGDFALTQNQ
                     TATHTYTFNDTSCVSTNTVAQIDPSDIVIGLYNDTIKLKLHFEWEKKGSITLSNNQTS
                     FTSGYNVTVTSAASDAIVNLSGGGSGSLMINSVATLSSASSSTRLAAFLQFAGCFLGG
                     TSWDICVQNYKNALSKNAGVYSFNLALSYNPITTTCKPDDLLITLESIPILQLPTTGN
                     KATINSKQGDIILRCKNLLGQQNQTSRRMQVYLSSSDLLAGSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(3101..5580)
                     /gene="yehB"
                     /locus_tag="ACHK1J_004542"
                     /pseudo
     CDS             complement(3101..5580)
                     /gene="yehB"
                     /locus_tag="ACHK1J_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            complement(5596..6270)
                     /gene="yehC"
                     /locus_tag="ACHK1J_004543"
     CDS             complement(5596..6270)
                     /gene="yehC"
                     /locus_tag="ACHK1J_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7913715.1"
                     /translation="MKGLSSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(6350..>6874)
                     /gene="yehD"
                     /locus_tag="ACHK1J_004544"
     CDS             complement(6350..>6874)
                     /gene="yehD"
                     /locus_tag="ACHK1J_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913716.1"
                     /translation="AAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSV
                     SINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDK
                     AIASSNDTVGYYLYLGDNSEVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDSSVNS
                     GDVLSSLVMRVAQD"
BASE COUNT         1898 a         1636 c         1348 g         1992 t
ORIGIN      
        1 aaaaagttaa aaaagaatac atagcttgat tccttacgtc ttacttcatt atataataac
       61 aatatagtat ccccatccag tgaatcaagt taaactaaat tgcatactaa taggtaaaat
      121 caatatgctt agatatactt tcaacgaata acactatctg tataatgctc tgattggtga
      181 tagttataac tcctatttat ttgatatatg aatccagcac cttcagaacg acatccagat
      241 cttcttcacg ttttagctca tccccctggt gaacgatgtg ttccgtcaga tgacctttaa
      301 tcacttcccg catcagaccg tttaccgcgc cacggatagc agcaatctgt tgtaaaactg
      361 cagcgcattc gtgcggctcg tcgagcattt tcttgagcgc cacgacctgg ccctgaatct
      421 tactggcacg cgctttcagt ttctgtttat cacggattgt atgagacatg gcaacacctg
      481 gttaacaaga atatgaaaaa tcatagcact attaatctac tggggggtag tatcaggtac
      541 tgggggggag tagaatcaga ttgccgaatt aatactaaga attattatca tgaccgaatt
      601 tacaactctt cttcagcaag gaaacgcctg gttcttcatc cccagcgcca tcttacttgg
      661 tgcgcttcat ggcctggaac cagggcactc aaaaacgatg atggcggcgt ttatcatcgc
      721 catcaaaggc accattaaac aggcggtaat gctcggactg gcagcaacca tttcgcatac
      781 cgcagtggtc tggttgattg cctttggcgg gatggtgatc agcaagcgct ttactgctca
      841 atcagcagaa ccgtggctcc agctgatttc cgcagtgatc attattggca ccgcgttctg
      901 gatgttctgg cgtacctggc gcggcgaacg caactggctg gagaatatgc acgagcatga
      961 tcatgaacat catcaccacg atcacgaaga tcaccacgac catggacatc atcaccatca
     1021 cgaacatggc gagtatcagg atgcccatgc acgagcccat gccaatgaca ttaaacgacg
     1081 ctttgatggt agagaggtca ccaactggca aattttgtta tttggcttaa ccggtggcct
     1141 tatcccctgc ccggcagcaa ttaccgtgct gttgatatgc attcagttga aagccctgac
     1201 actgggcgca acactggtcg tcagtttcag cattggcctg gcgttaacgc ttgtcaccgt
     1261 aggcgttcgc gcagcaatca gcgttcaaca ggtcgcaaaa cgctggagcg gatttaacac
     1321 tctcgctaaa cgcgccccct atttttccag tctgttgatt ggcttagttg gtgtgtatat
     1381 gggcgtacat ggcttcatgg gcataatgcg ataactcaat cctttaggct tgcatgttat
     1441 cttcagccca ggatgattct taaatcagct atttccgctg actctctgct cgataaggat
     1501 gcttttaggg catcctttca tttacacttt ttacgaaatc atgggatcac taacaaaata
     1561 tcgcttgtca gttatattgt atggcaggaa agatatgcga ctgatattac agatccccaa
     1621 agtggagagt ttatgaccat taaaaataag atgttgctgg gtgcgctttt gctggttacc
     1681 agtgccgcct gggccgcacc agccaccgcg ggttcgacca atacctcggg aatttctaag
     1741 tatgagttaa gcagtttcat tgctgacttt aagcatttca aaccagggga caccgtacca
     1801 gaaatgtacc gtaccgatga gtacaacatt aagcagtggc agttgcgtaa cctgcccgca
     1861 cctgacgccg gaacccactg gacctatatg ggtggcgcgt acgtgttgat cagcgacacc
     1921 gacggtaaaa tcattaaagc ctacgacggt gagatttttt atcatcgcta aaaaaagccc
     1981 cctcatcatg agggggaaat gcagacacct tgttattttt tattattagc cacttgctcg
     2041 tcttgcttgt tattagtcgt atttcacgtt gattaatgcg gttgcctcca gtgcgccaga
     2101 tttaatttta tttgtatcgt agacgtagta actggcagtt atcggaattg aaaccgttgt
     2161 atctgtaagt tttgactttg ccgcaatttc ttttaaattt gtgtatcctt tactgctgtt
     2221 agtgatattt aaaagtgtga ctggcgaacc attactttca agaataaatc cgactccatt
     2281 atcttctgca cctttcagga tcgtgttact gccggctaac aaatcagaac ttgataaata
     2341 cacctgcatt ctccgtgatg tttgattctg ctgacctaat aaatttttac agcgcagaat
     2401 aatatcccct tgtttactat ttattgttgc tttgttacct gtggttggta attgtaaaat
     2461 tggaatactc tctaaagtaa ttaataaatc atcaggtttg caggtcgttg ttatcgggtt
     2521 atatgacaat gccagattaa aggaatagac acctgcattt tttgataatg catttttata
     2581 gttttgcaca cagatatccc atgaggtgcc gcctagaaaa caacctgcaa attgtaaaaa
     2641 agcagccaaa cgagtagatg atgaagcact gcttaatgtc gcgacactgt taatcatgag
     2701 agatccactt cccccaccag acaaattcac tatggcatcg ctcgctgccg atgtcacagt
     2761 gacattatat ccactcgtaa aactagtttg gttatttgaa agcgtgatgc tacctttttt
     2821 ctcccattca aaatgcaact taagtttgat ggtgtcatta tagagtccaa tcacaatatc
     2881 cgaggggtct atctgcgcta ctgtattagt actgacgcaa gaagtatcat taaaagtata
     2941 tgtatgagtt gctgtttgat tttgagttag cgcaaaatcc ccctgaacaa ccaaattatg
     3001 ctgtgctgac aactcgccat aacatgcagc ataactcaca ggcataacca tcatcattac
     3061 tataaataac attatacgaa tattcataac cacctgacta ttactggcaa atataattcc
     3121 tactttcatc aatctcttta ccgaaggtga ttgtgcatga aagtccttgt tgcttatcaa
     3181 ttgctacatt aaccgatggc ggtacttcat tggtgcgaat aaataactga cttccctggc
     3241 cgacaacgcc aatattatga ccatggatat cattgacttc ataaccaaac gttaatggtt
     3301 gcccatccgc tcttaacgct tttataaacc agggcttgcg ctgatcggta tcaaaattaa
     3361 ccagtacaac cgcgccgcga taagaggcgg caattttccg gttaccgcgc aattctgctt
     3421 cgctatcgct ttgcgacaca tccagcatca ggtgattttc ccgataaggt gtcattccgt
     3481 cgtataccac cactccatta cggtttgttg tgcgatattt ttgcccattg acataagcat
     3541 ctttaattcc tggcgcattc atcacagcaa acgtttcgga aagacggttc gccagattaa
     3601 cgccacccga ccaggcgaca atgccccctg aaacactggc tccagcctgt cgataagtac
     3661 tcgactgact ataactgcca ttcactgtcg caaccggcgc gttccaggtt aaattcgctc
     3721 cagctgtcgt ttcatttccc tgatgttgat gactcaggtt gacaccataa ttgaactgat
     3781 cccgactccc tactgttcct gataatcccg tattatttga ggcaaacccc tgatcatcaa
     3841 acgtcgttga gttagacata tatatttgcc gacgaggcgt cgaaacgtca tcaccccaat
     3901 caaagggaat cgatataaaa atattaaaac gtttctcttc atgatgattc tcgtcataag
     3961 cctggcttgc cgcgagggta tagcttatcc gtcgcaggtt gttggaataa ctcaactgat
     4021 aatccttact actgccgcta cgcccccagt aatctcgcca taacgtactt aatgacacag
     4081 aaccccagcc ttctggcaat gactggctca tattggcaga aaagctattt ttacggccaa
     4141 aatcgttctg gtaataatcg gcaatgtcat agacatcgtt ttcatcacgg cgataattat
     4201 ctttattgtt agcccaaacg tgatcattaa atgtccgata atcacgcgac gaataacgca
     4261 ggccgccaga ccaaaacgcg tcgacgtttg gctcacaaat ttgttgtagg caatttgata
     4321 actttgcccg tcaaacacat cgccgttgtc ttgtttacta tgcgacttcg tggcatcgac
     4381 ggaaatggca ccaatgcgtg tattccagcc agtccccaaa gtgaacgcgt aataattatt
     4441 cgcgaccatc gagccaccat acagcgtcaa taagttatta aaaccatact gataacccgc
     4501 ctggacaaaa tcactttgtt tgctcgcccc ttcaatatgg ctacggcccg ccgcaaaatc
     4561 atatttcgac acgccgggtt gcagcatatt tggcacggct gcataaggca ccagataggt
     4621 ggttaccgaa ccgtccgcct ctttcacgct gacatcaaga tctgctccac caccggccaa
     4681 ctgcaaatct gtaatcgcaa acgggccagg aggaacctct ttctgataaa ccacaaaacc
     4741 attctgttca atagttacca gcgcgttact ctgagcaatc ccctgcaccc gtggcgtaaa
     4801 attttgtttc gagttaggca acatctgcat atcacgaaac aaccgcacac ctctgaagcg
     4861 aacagaatca aaaatatcgc ttgatgtgta catatcaccc acgcgaagcg tgccgagaag
     4921 ttgggcaaat ccacgttcca gatacagggt attgcttttc cacgcccctg gattgttatt
     4981 tgttttactg aaactggcat cagaatgcag ttgccacccc agtaaattta acccgctgtt
     5041 aaaacgtaca tatgtactct tactattacc cgacgcttta tagtcactgt aatactgact
     5101 cacataataa gaggtataaa acgcattaat accccgctcc cagttttccg gtggaacata
     5161 gccactttcc agttcttcca cccaggcctg cgggacactg aaatcgagac gaaaaacccc
     5221 gatatcccag gtatagctcc caccctgaac aagttgctca aatgttaaac attgcttacc
     5281 gctggcgaag ttatcgctat taatgcctaa ccgcttgata atttcccttg ataaacatgt
     5341 ttcttgcggg ttgtctttaa caatgatctc atatttcccg cgccattgct tattgacata
     5401 aatatcgatg tcatactgcc ccggcaaggg ttgattatca tcaagacgaa tatttgcaac
     5461 ctgctggtct ttcattccac ctatcataaa atgggtatca aaggtttctt cagctgcata
     5521 agcttcaatg ccgagcaata acgctacgat tgctgatgca agtggggtca ttctcaacat
     5581 aggcagctcc tgcaattaaa ttttgtcact aatatagttg ccatggtcat cgataatggt
     5641 cagatgccag ttatttgcat tattactttt gacattaaca ctctgacttt ctaagggggc
     5701 aatcataata gtttcataat tgactttgac attattagct ttgacatccg aaatcgtcac
     5761 ccaattagct gagtcatttt ttatcaccaa tccattgcca ctgcgattta ccagtaactt
     5821 tttaaaggtc gctttattta ccggagcaat acccgccggc cggtaaaaca atttgattct
     5881 gttttgcatc gcaaacttca gtgcattctt accttcttgt tctggactat ttggtggaat
     5941 atccagaaca ttcagataaa aaatgctttc tttattagtg ggcagtttat tcggcataat
     6001 tttgattttt acttgctgcc cggaatttgc ccctattttt gccactggtg gcgttaacat
     6061 gaaaggaacc tgaatttttt ctggtggtaa tgacgtatcg ccatcatcaa tccacgcctg
     6121 cagcagcgaa gaacggtttc cctggttcat caactgcacc atcacttctt tattttctgc
     6181 cgggtaaata atgcgcgtcc cgtagataac aattccggca tgtgcaggaa ggaccataga
     6241 aaaaatgagt aaagatgata atcctttcat tttaatgcct cttaaattaa aaggccgcca
     6301 tgggatagcg gccatgttta ttacctggag aaacttacaa ttttatttat taatcctgcg
     6361 ccacacgcat aaccagagaa gataacacat cacctgaatt cactgagcta tctgtcagtt
     6421 tcaggtattt tgctttaaac ggaatagcat attgaccttc cgctgtttta taactctcaa
     6481 tattaaaagg tgcagaaaca tccaggactt cgctgttatc acctaaatag agatagtaac
     6541 ccacagtatc gttgctactg gctatcgcct tatcgttacc agttatttta tcgttggtaa
     6601 tcaacgagag ttttacacca gcaacttctt tcgggcagct aatttttaac atgctgctgg
     6661 ttacaccgac tgcatcatca taggtagaat atttagcttt accttcaaag acagtggttg
     6721 gtacagttgg catattaata cttactgaat caccacccgc ttcgaattta cacggagatt
     6781 ctgcaatatt tcctttaatg gttaatgttc cggtatcaac gtctgcagca aatactccag
     6841 cgctcataaa aaaagaagaa aagacagcgg cagc
//
LOCUS       DAZGBC010000212         6851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGBC010000212 DAZGBC010000000
VERSION     DAZGBC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.213"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..66
                     /locus_tag="ACHK1J_004545"
     CDS             <1..66
                     /locus_tag="ACHK1J_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913717.1"
                     /translation="KALIQLRRFTGFKNVRLYQLH"
     gene            109..669
                     /locus_tag="ACHK1J_004546"
     CDS             109..669
                     /locus_tag="ACHK1J_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913718.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLLPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            755..1456
                     /locus_tag="ACHK1J_004547"
     CDS             755..1456
                     /locus_tag="ACHK1J_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7913719.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVVIRAAELLSNGKKVPLANSVMIAPQSTTEWTLRSGISVARGALIHLV
                     TVNDYGVNVTSEHAL"
     gene            1509..4031
                     /locus_tag="ACHK1J_004548"
     CDS             1509..4031
                     /locus_tag="ACHK1J_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7913720.1"
                     /translation="MNKSVVSISAAMLVLLYQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVATTNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDFTWQHGLTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGVTFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSEKQVAP
                     VAGAAVKVVFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            4047..5381
                     /locus_tag="ACHK1J_004549"
     CDS             4047..5381
                     /locus_tag="ACHK1J_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913721.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWATCSNTRVGQTEDGRSALI
                     EFGKINLTDTYFAPVGSLLATTVVPSTNYTSGGASGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFHNAGGPDGLSDVYATWFAFVGLKQTMAGVTIGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTAGATSYCGNTNTDGDGVGYAKPSGTPYNCTQPNAY
                     IQLSGTSGIPFAHDEVGEDSADHYDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAYPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5378..6067
                     /locus_tag="ACHK1J_004550"
     CDS             5378..6067
                     /locus_tag="ACHK1J_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7913722.1"
                     /translation="MKSVLLLLTVLFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6213..>6851)
                     /locus_tag="ACHK1J_004551"
     CDS             complement(6213..>6851)
                     /locus_tag="ACHK1J_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7913723.1"
                     /translation="IKEPDNKYLKHLTHEGVEVEFMTIDITKPDAACTIINRCLERFG
                     QLDILVNNAVICNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKVINM
                     CSVLSFHGGRWSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYN
                     NPKIKELIKGRIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1817 a         1693 c         1619 g         1722 t
ORIGIN      
        1 aaggcactta tacagttaag gagatttacc ggttttaaaa acgtcagatt gtatcaattg
       61 cattaattat ctcgttctat attaactcta aatttcaagg atattaatat gttttctact
      121 ttcaaaaaag cagctctgct ggcagctatc gcattacctt tttctaccat ggctgcaccg
      181 acagtcactt ttcagggtga agtaaccgat cagacctgtt ccgttaatat caacggtcaa
      241 accaattcag tagtattgct gccgaccgta gccatggctg acttcggtgc aactttagct
      301 gatggccaga gcgcaggcca gacgccgttt acagtttctg tgtctaactg ccaggctccg
      361 actggtgcag atcaggcaat caacaccact ttcctgggct acgacgttga cgctagcacg
      421 ggtgttatgg gaaaccgtga taccagcagc gatgcggcga aaggctttgg cattcagtta
      481 atggattcca gcacttctgg taacccagta actctggctg gcgcgactaa cgtaccgggt
      541 ttgaccctga aagttggcga tactgaagcc agctacgact tcggtgcgcg ttacttcgtt
      601 atcgatagcg cagctgccac tgccggtaaa attaccgctg tcgcagaata caccctgagc
      661 tacctctaat taatctactg gctggcagta aatgccagcc agtatttctt cgcacaatta
      721 atagtgattt ttcatggaca tggaaaagat aagaatgaac atttttgcat atttactggt
      781 acttgtattt tccatgagca tgagcagcag cgcgtttgcc agcgtggtaa tgaccggaac
      841 ccgtattatt ttccctggtg acgcaaagga aaaaaccatc cagttgcgaa ataccagcga
      901 tcagccctat atcattaata tccatgttga ggatgaacgt ggttctgaca agaatgtacc
      961 gtttatgcca accccgcaga catttcgcat ggaagctgcc gcaggtcagg cgttacgcct
     1021 gctctacact ggtaataatt taccgcagga tcgcgagtct gttttctggt ttagtttcag
     1081 tcaactacct tatctgaata agaatgataa aagtcagaac cagctcatcc tggccctgac
     1141 taatcgagtc aaaattttct atcgtcccag ctcgattgtc ggtaaatcca gtgacgcacc
     1201 caaaaacctg acttaccagg taaaacagaa ccgcattgaa gtgacgaatc ccacgggcta
     1261 ttacgtcgtg attcgcgccg ctgaactgct tagtaatggt aaaaaagttc ccctcgcgaa
     1321 ttccgtcatg attgctcctc aaagcacaac tgaatggaca ctacgctctg gcatcagtgt
     1381 cgctcgcggt gcgctgattc atttagtgac cgtcaacgac tatggcgtaa atgttacgtc
     1441 tgagcatgcc ttataacctc tttctccttt tttgagcggt atcctttttc acgcacgagt
     1501 acgtcgagat gaataaatcc gttgtgtcaa tttctgcggc aatgttggtt ttactttacc
     1561 aaccggtcat ggggagcgaa atctcacccg caacaccgtc agatgaagac aactacacct
     1621 ttgacccgca actcttccgc ggcagcagat ttagtcagtc gtcattagca aaactgacaa
     1681 cacgtgagtc tgttgcaccg ggcaattata aaatggatat ctacaccaac aataagttgt
     1741 caggcagttg gaatgtcacg tttaaagaag ccgctgatgg tcgcgttctg ccctgcctga
     1801 cgcctgaagt cgcggacgcg ataggcctca aaacagggga agataagggg gaaaaagatc
     1861 ctgtctgtac gtttgctaag gaactcgctc ccggcatcac cagccagaca cagttgtcac
     1921 aattgcgcct ggacttgtcg gtgcctcaga gtcaattgat tagtcgccct cgcggctatg
     1981 ttccccctag cgagctggat accggagcat cgctggcgtt catgaattat attgccaact
     2041 attacaacgt tgcctattca gggcagaatg ctcatagcca gcgttcgcta tgggcatcat
     2101 ttaatggcgg cattaacctt ggtgcctggc aatatcgtca gttatccaac atgacctggg
     2161 ataacgacaa agggaatcag tggaacaata ttcgtagcta tttgcaacgc ccgctgcccg
     2221 ccataaatag tcaattaatg atggggcaac ttatcaccag cggaagattt ttctctggac
     2281 tcagttatca cggcgttagt ctcgcgaccg atgaacgtat gctgccggac tccatgcgcg
     2341 gctatgcgcc gactattcgc ggcgtggcta caacaaacgc cagagtctcg gtaatgcaaa
     2401 acggtcatga aatttatcag actaccgtgg ctccaggccc tttcgaaata aacgacctat
     2461 accccaccag ctacagcggc gatctggatg tcaccgttac ggaagctaat ggcgcagtca
     2521 gtcgtttcag tgtccccttt tcagccgtac cagaatcgat gcgtccagga acttcccgtt
     2581 ataacgttga agtaggtaaa acgcaggata gcggtgatga ctcgatgttt ggtgacttca
     2641 cctggcagca cgggctgact aatacgctaa catttaacag tggctcgcgt attgctgatg
     2701 gctaccaggc gctgatgctt ggcggagtct atggcagttc gctgggggca tttggggcaa
     2761 acctcacttg gtcccatgcg cgtgttcccg aaagcgaagc acagagtggt tggatgtcgc
     2821 aattaacctg gagtaaaact ttccagccta cttcaaccac cgtctccctg gcaggttatc
     2881 gatactctac cagcggctat cgtgatctgg ctgatgtgct gggagagcgt catgctgcca
     2941 gtaataaaca gtcatgggac tccagccagt ggcgtcaaca gtcgcgcttc gatcttacgt
     3001 taagtcagag ccttgcgaat tacggcaacc tgtttgtgtc aggttcaaca cagaactacc
     3061 gtggcggcaa gagccgtgat acacagcttc agttaggtta cagcaatagc tttagccacg
     3121 gcatcagtat gaacctttcc gtcggacgcc aaagaatggg cggctataaa gacaattctg
     3181 atgatatgca gacggtaacg tccctttcat tctcattccc acttggcggc aatggacctc
     3241 gtgtaccaag tcttagcaac agctggaccc attcaactga cggtagctcg caattacaaa
     3301 gctcgctaac cggaatgctt gatgaagcac agaccaccaa ctacagcctg aacgtcatgc
     3361 gcgatcaaca atataagcag acgacgctta gcggaaacat gcaaaaacgt ttttcacaaa
     3421 ctaccgtcgg attgaacgca tcgaagggcc aggattactg gcaggcgtcc ggaaacgtac
     3481 aaggcgcgat ggccgtccat ggcggcggcg tcactttcgg accttatctg ggtgaaacgt
     3541 tcgccctggt cgaagcaaaa ggcgcagaag gtgcaaaagt ctataactcc agtcagctgg
     3601 aaattaatga cagtggctat gcgcttgttc cggcagtaac gccctatcgc tacaaccgta
     3661 tatctctcga tccacaagga atggatggcg atgccgaact ggtcgacagt gaaaaacagg
     3721 tagcaccggt tgcgggtgcg gcggtgaaag tagtcttccg tacccgtcct ggtaaagcgc
     3781 tgctgattaa atcccgcatg gcagatggtt cggaactgcc aatgggagcc gatgtgctgg
     3841 atgagaataa tactgttgtc ggtatagccg gtcagggggg gcaaatttac ctccgcacag
     3901 aacagacaaa aggccatttg tcagttcgct ggggtgaagg cgctaacgat agctgccagt
     3961 tgccctttga tatcagcggg aaagacagca atagccctat catccgcctg aatgaaacct
     4021 gtcagtcttg atacagagaa aacccgatgt ctaaatcgac ttttttacac attctgatta
     4081 gctccatcat attggtggca ctaatacaat catccgcctg ggccacttgt tctaatacac
     4141 gtgtcggaca aacggaggac ggaagatcgg cactcattga gttcggaaaa attaacctga
     4201 ccgacaccta ttttgcgcca gtaggctcac tactcgcgac aacagtcgta ccttccacta
     4261 actacacatc aggtggcgcg tcaggaagct ccgtactgtg ggaatgtgac gcaacagatt
     4321 tgccaaacat ctattttctg gtcgcaacca atggcgacga ccgcgtgggt ggctttcaca
     4381 atgcaggcgg gcctgatggt ctgagtgatg tctatgccac ctggtttgct tttgtcggtc
     4441 tcaagcagac tatggcgggc gtgacgattg gtcgttactg gaaaaaagtg cccatcacta
     4501 gttatgccac tcagggaact aaaatccaga tccgcttaca ggatatccct cctcttcatg
     4561 ctgagcttta ccgtattagt acgctgcctg ataccgctgg cgcaaccagt tactgcggta
     4621 ataccaatac tgatggcgac ggtgtggggt atgctaaacc ctccggcaca ccttataact
     4681 gcactcagcc taatgcctat attcagcttt ccggtaccag tggtattcca tttgctcatg
     4741 atgaagtggg cgaggacagt gcggatcact atgatttttg gggtgctgat aatggttttg
     4801 gttacggaat gcgttcggcc aatcgactct acaacaatgc cacctgcgtt gcccgcagcg
     4861 ccacgccgtt agtattgctg ccgacaattg cagaagcaca actgaatgcg ggcatggaaa
     4921 gtaccggtaa ttttaatgtc cgcgtcgagt gtagtaactc ggttcaatca gggattagcg
     4981 atactcagac tgcattagga atccaggtgt ctgaaggtgc atatacagcg gcgcaaaaac
     5041 tggggattat caatagcaac ggcggcgtca gcgccctagt ctctgataat tatgacgcag
     5101 cagagatggc aaagggcgtt gggatctaca tctcaaacag tgcttacccc gatacggcga
     5161 tgacgctggt tggtcaaccg ggcatcgcga agttaacccc cggaggaaat gcagcggggt
     5221 ggtatcctgt atttgaaggg gcaacattag aaggtgcgac tcaccccgga tactccagct
     5281 atagttactc ttttatcgcc cggttgaaga aactgccaaa tcagacagtc agtgcgggaa
     5341 aagtgcgggc aacggcgtat atattggtga aaatgcaatg aaatccgttt tgctgctctt
     5401 aaccgtcttg ttcgtacagt cagtggaagc cggcatcatg ccggcttctt cacggattat
     5461 ttatcgcgat ggcgatcggg aaaagacact gatgctggtc aataccaatg actatccggt
     5521 actggtgcaa agttgggtag ataatggtga aggttcccca gaagctgctt ctcccccctt
     5581 tgtggtgctg ccaccggttt tccgcctggc tgcgagacga acccaggggt tgcggattat
     5641 ctataatcat gacccacttc cccaggatcg cgaatctgca ttctggttaa atctttatga
     5701 aatgccgcca gaaactcccc atcaagatcc cacacattta acgctggcaa tgaatacgca
     5761 acttaaaatc ttttacaggc caaaatctgt aactatgacg ccccaagaag ctatgcgtaa
     5821 aataagttgg catctgggta acgacggcag cagtagctat attgagctta ataacccaac
     5881 accactgaat atctctttca cctccatcag cattggggct acaaaggtgc agcaagaaag
     5941 cgatatgatg ataaaaccct tcagtcaacg acggtactgg ttagcaagta ataacgttag
     6001 tggtgtggtt gcggcgacat atctcgatga taatggtgca cagataaaac aacaattagc
     6061 gcaataatgc tctgagtggt atgtaaactc atcgcgatgg ttcaacgcta acgaataacc
     6121 actataccca cctgaagtac cggaagtgga gaaatgacaa aatctctcca ctccattttc
     6181 cagtcaaaac acctcaacgc agccaaaaat cgttaacgaa ttgaatatcc accatcaatg
     6241 actaatagtt gtccattgac ataatctgaa gcggcactgg cgaggaacac cattgcgccc
     6301 ataagatcct gtgcccttcc ccagcgttga gccggtatac gcccttttat taactctttt
     6361 attttagggt tattataaat aatagcggtc atttcactca cataatatcc tggggcgata
     6421 ccattaatct gaatattata ttccgcaaaa tcatcggcat acgctttggt cagccccgct
     6481 aaagcatgtt tagtcgccgc ataccctggc gaccaacgtc ccccatgaaa tgacaatacg
     6541 gaacacatat taataacttt cccttttctt tgcgggacaa atatattcaa tgcggcctgg
     6601 ctcatatcga atgcagcatt caggttgaga ttaatcatcg gatcccagtc gtttcgattg
     6661 aaatcgatag gacgattaat gttacaaatc accgcgttat tgaccagaat atctaactga
     6721 ccgaaacgct ccagacacct attaataatg gtgcatgctg catcaggttt tgttatgtct
     6781 attgtcataa actcaacttc aacgccttca tgagttaaat gctttaaata tttattatca
     6841 ggctctttga t
//
LOCUS       DAZGBC010000213         6704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGBC010000213 DAZGBC010000000
VERSION     DAZGBC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.214"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            85..1851
                     /gene="adeD"
                     /locus_tag="ACHK1J_004552"
     CDS             85..1851
                     /gene="adeD"
                     /locus_tag="ACHK1J_004552"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO7913724.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1899..3290)
                     /gene="uhpT"
                     /locus_tag="ACHK1J_004553"
     CDS             complement(1899..3290)
                     /gene="uhpT"
                     /locus_tag="ACHK1J_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO7913725.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3428..4747)
                     /gene="uhpC"
                     /locus_tag="ACHK1J_004554"
     CDS             complement(3428..4747)
                     /gene="uhpC"
                     /locus_tag="ACHK1J_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO7913726.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4757..6259)
                     /gene="uhpB"
                     /locus_tag="ACHK1J_004555"
     CDS             complement(4757..6259)
                     /gene="uhpB"
                     /locus_tag="ACHK1J_004555"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO7913727.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6259..>6704)
                     /gene="uhpA"
                     /locus_tag="ACHK1J_004556"
     CDS             complement(6259..>6704)
                     /gene="uhpA"
                     /locus_tag="ACHK1J_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO7913728.1"
                     /translation="VCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNA
                     GARGFLSKRCSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQ
                     GMAVKEIAAELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         1696 a         1918 c         1738 g         1352 t
ORIGIN      
        1 cattaatgga tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat
       61 tgataaaatc aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt
      121 agccgggctg aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat
      181 attattgata atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt
      241 gtgattaaag gacgttacat tgccggtgtt ggcgcagaat acactgatgc tccggctttg
      301 cagcggattg atgcccgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat
      361 attgaatcca gcatgatgac gccggtcacc tttgaaaccg ctactctgcc gcgcggcctg
      421 acgaccgtta tttgcgaccc tcatgaaatc gtcaacgtga tgggcgaagc cggattcgcc
      481 tggtttgccc gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc
      541 gtgcccgctc tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc
      601 gcctggcggg accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta
      661 attagcgggc agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac
      721 ggtcactgcc cgggtttggg tggtaaagaa cttaacgcct atattgctgc gggtattgaa
      781 aactgccacg aaagttatca gctggaagaa ggacggcgga aattacaact cggcatgtcg
      841 ttgatgatcc gcgaagggtc cgctgcccgc aatctcaacg cactggcacc gttgatcaac
      901 gaatttaaca gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggaaatcgct
      961 catgaagggc atatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg
     1021 catgtggcat atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc
     1081 ggcttactgg cacccggaaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc
     1141 acggtgcagc aggtactggt gaaaggcgaa ccgattgacg cgcaaacctt acaggcggaa
     1201 gagtcggcga gactggcaca atccgctccg ccgtatggca ataccattgc ccgccagcca
     1261 gtttccgcca gcgactttgc cctgcaattc acgcccggaa aacgctatcg ggtcattgac
     1321 gtcatccata acgaattgat tacgcactcc cgctccagcg tctacagcga aaatggtttt
     1381 gatcgcgatg atgtgtgctt tattgccgta cttgagcgtt acgggcaacg tctggctccg
     1441 gcctgtggtt tgctcggcgg ctttggcctg aatgaaggcg cgctggctgc gacggtcagc
     1501 catgacagcc ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc
     1561 aatcaggtga ttcaggatgg cggcgggttg tgcgtggtac gtaacggcca ggtacaaagt
     1621 catctaccgt tgcccattgc cgggctgatg agcaccgaca cggcgcagtc gctggccgag
     1681 caaattgacg ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt
     1741 attcagatgg cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg
     1801 ctatttgatg gcgagaagtt tgccttcacc acgctggaag tcacggaata ataaaaaaaa
     1861 agcccggcgt catgccgggc aaaagtcacc agttacgttt atgccactgt caactgctga
     1921 atttttttct cgcggcggat tttgcgttct tccatcaccg ccactatcgc catcaggcag
     1981 atacaaccaa tcgcggcgat atcaagtgcg gcgaaggtgc ctgcccagcc ggtaaggccg
     2041 aataccggcg tcccatcggc aatcattccc agacctaact tggcaaagct gtcaccaatc
     2101 aggtaagcaa aggtgccttt aataccatcg gcagcgccaa tcgctttttt aggtacaaag
     2161 ccaacagcag ccacaccaat caacaattgc gggccaaaga ccaggaaacc caacgcaaag
     2221 agagaagcca gatagatata ttcgttgctg gcatgttgat acacaccgag cgtggcgata
     2281 atcagcgcca gcgcgatgca ggccaccagg ccacggcgac cgttcgccag gtcagagagc
     2341 cagccccaca gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa gccctgaatc
     2401 gccaccgctt tagagagttt cagttcctgg aacgcgtata cggttgacca ctggtcgata
     2461 ccaatacgta ccacatagag gaaaatgttg gcgaagcaca gcagccagat cactttgttt
     2521 ttcagcacat actcaacaaa gatctgccac ttggtcatat cggtagattc tgtctctttg
     2581 tcctcttcgc tgatctcctc gccgaacagt tcttcagctt tgccgaggcc ataagattcc
     2641 ggggagtcgc tgccgtaacg caggccgata aaaccgacaa tcagcgcgat aatcgacggg
     2701 aagataaaca tgccgatgac atggccatcg aacaggtaat ttgcgccgaa cagcgccaca
     2761 cctgctgcgc ctgcaccgcc aaggttgtga gatatgttcc agaaaccgag gaaggtcccg
     2821 cgcttacgac gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc gccggtgctc
     2881 tggaaaaagc cgcttaaggc gtagaaggca atcatcagaa acaggctaac cgagccgctg
     2941 cccatactgg cactgaagcc cagcatacaa atggcagaca ggataagcat gaacggcagg
     3001 aattgtttgg tgtttttgcc gtcggcgtag taggaaacca gcgttttacc cacgccataa
     3061 gtgatggaga aacccaggcc gatcatcccc aactgcgtca tgctcaaccc gtaggtcgaa
     3121 atcatatcgt tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt caggtagccg
     3181 ataaagacca ccaggtagga ttgcatgaac ggtttgaacc acattttgcg ccgcacttcg
     3241 agcggaaggt ccagggtcgg cttgcgaacc tggtttaaga aagccagcat gggttactcc
     3301 tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc aggcctgaga
     3361 tagcgtccag gcaaaacctg gaaaatttct tagttttgcc ggatatgaag tgaaaaggtg
     3421 agatgcatca cgcttcgcgc ggtgcctggg cgttcaaaaa gggcagtaac agcagtgcgg
     3481 aaatcccggc ggcgatagag ataaccacaa aaaatccgct ccagtgccag gtatcgagta
     3541 ctttcgccag tggccaacca gcaagcgacg cccccagata agcaaacaag ccgacaaacc
     3601 ccgtcgccgc ccctgccgcc tctttgtggg aacactctgc cgccgccatg ccgattaaca
     3661 tctgcgggcc aaagacaaaa aaaccaatag tgaagaagca ggttgcctgc atcacgtagc
     3721 tggcaaatgg catcagccac agcgagccga ctgaaagcaa aattccggcg gcgaaaatca
     3781 aattcatcgg cccacggttg ccgttaaaca atttgtccga gccccaaccg gctaccagcg
     3841 caccgataaa tccgcccagt tcaaacatcg tcactgccgt attcgccgtg accagatcga
     3901 cgcccagcgt ctcggacata tacaaattgc cccagtcgtt gattgccgcc cggaccacat
     3961 agaccagcac atagcaaaac gaaagcagcc agatatacgg attcagcaac acatacttgg
     4021 tgaggatctc tttacgcgtc agccctgccc cttcttgttg ttgagcaatc tccagcgcgt
     4081 cgtgccgcca ttcacctacc gccggtaaac ctaacgcctg cgggcgatcg cgcagccgcc
     4141 agcagagaaa aatccccacg acgatcgcca tacaaccagc aatcatcatc ccggcacgcc
     4201 agccgtaatg tagtgcagcc gctgccatca caatgggaat gagtgcgccg ccgacgttat
     4261 gcgccgtgtt ccataatgcc caccaaccgc cgcgctcggt acgagaatac caggcggtta
     4321 acagacgcgc acacaccggt gaaccccagc cctggaaaaa ggcgttcagc acccagagta
     4381 cggcaaacgc ccatagcgac gtcgagaagc caaacagaat gttgataatg cccgtggcga
     4441 taagccctat ccccataaaa taacgggcat ttgagcgatc gctgacaatg ccggagacaa
     4501 acttcgacac gccgtaagta atgtaaaaca gggtcgctaa caggccgata tcgctacggc
     4561 tgagcacgcc gttagcaagg atttctggta cggcggcgtt aaaacttttc cgcgtgaagt
     4621 aaaacagcgc gtaacccagc cagatggtca gcagaatatg ccgacgccag tagcgatagc
     4681 gtgcatcaat ttcatgtttg tcagtcatta atggcgcatc ggcaggcgct ttcagaaacg
     4741 gcaacatcat cgaaccttag acataacgtt gaggtagaga aacgctgaca cgcgtgccgt
     4801 gcagacagga aatgtgtaat gtgccgccca gcgccgttac gcgctcgcgc attccggtga
     4861 ggccaaaacc ttgttgcccg gaacccggcg gcaaaccgct gccatcgtct tcaatcacca
     4921 gcatcaaccg ttcatcctgc tgccagcctt gcagcgtaac cgcgctggca tcggcatgtt
     4981 tcacaatgtt gttcagccct tcctggcaga cgcgaaacag cgtcacgcgc tggttttcac
     5041 ttaacgctga ctcatcgata cgccattcga gatggctgac aatgccgcgc ccttccagct
     5101 ccatttcccg catcagtgag cggatggcct gctccagggt gagatcatcc agctggcgcg
     5161 gacgtaaccg ccctaacaaa cggcgcaccg catcgtaaac acccagcgac agttgttcga
     5221 tgagctgccc gctctgcttc acgctggcgt tatctgccgc cagtcgctgg acaatgcccg
     5281 cctgagtacg aatggcagtg atggtctgac cgatatcgtc gtgcagctca cgcgccacat
     5341 cacggcgcac gctctcttcg gtttctaaca aacgttcagc cagatgctga ttgcgcgcca
     5401 gttccttttg tagcgactgg ttaagttcac gcaaccgctg gatgcctgcg cctagtaaca
     5461 accctgtcag actttgcacc agcaacgaga gcaataaatc caccggatga tcgcgccagg
     5521 tttgactggc gatcagcgcg atggcgttca tcaacgtcgc aatcagcgct ccttgccaac
     5581 catagtgcca ggccagcgca ataatcggca gcgccaggca gaatggcgta aagcgcgaca
     5641 gttcgtccgg caatcccaac tggagccaga gactaataac aaacagcagc aagtaccaga
     5701 ccagatgccg cccgcgccag ttgatcggtt gagaaactaa cgacgggccg agcggcagcc
     5761 aggtgttatt ggcgagatag tgccagaaca ccagacatat cggggccagc gtcaggccgc
     5821 cagttaaagt cagtagtagc gcattccacg actctttgcc gtgccaaagc cagggcagcg
     5881 actgcaacaa cgccgccgcc gttaacgccg ccccctgtaa cagcaaggtg cgccagtcac
     5941 gctgatggcg atagcgcgag atcagcgcta cgggcagtaa cgtcagtaaa ctaccgatca
     6001 tcaataagga aaaatgggtt aacccgaccg cctgcgttag ccagtaaatc agcagccatt
     6061 ccgcgcccag caatacgggc cagtatccgc gcgggcattg cagcattagc cccagacgca
     6121 gaccaaacgg aaataacagc accgccatat cagggcgctc aaccaggtgc aggctgatac
     6181 tccacaggca aaaccatgcg gcagagaaga taaaaaagca ggcaataacg gtaattaagc
     6241 gggagaacaa cgtcttcatc accagccatc aaacatgcgg cgcgccagtt caacgtcgtt
     6301 actgacgccc agtttttcca tcagattggc gcgatggacg tgtaccgttt tcggtgacaa
     6361 gcccagttcg gcggcaatct ctttcaccgc cattccttgc gccagttttt ccgccacctg
     6421 gcgttcgcgt ttggtcagcg ggtcctggcg accggatgcc agtttaatgg caatatccgg
     6481 cgtcagataa cagccgcccg tggcaaccgt atgcaccgcc gcaatcagtt cgtcagggct
     6541 acagcgtttg gagagaaagc cgcgcgcccc cgcgttaagc gcctgctcaa ccagcgccgg
     6601 actgtcatga acggagagca taattgtcgc catacctttc ggcagctggc ttagcagttc
     6661 cagaccggag atatcgggca tggagatatc gcaaatacac acct
//
LOCUS       DAZGBC010000214         6669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGBC010000214 DAZGBC010000000
VERSION     DAZGBC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.215"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..143)
                     /locus_tag="ACHK1J_004557"
     CDS             complement(<1..143)
                     /locus_tag="ACHK1J_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913729.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKISKMMNGKLIYSWLKWQCG
                     EFF"
     gene            221..1315
                     /gene="ddlA"
                     /locus_tag="ACHK1J_004558"
     CDS             221..1315
                     /gene="ddlA"
                     /locus_tag="ACHK1J_004558"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7913730.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1339..1551)
                     /gene="yaiZ"
                     /locus_tag="ACHK1J_004559"
     CDS             complement(1339..1551)
                     /gene="yaiZ"
                     /locus_tag="ACHK1J_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO7913731.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1811..2119
                     /gene="yaiY"
                     /locus_tag="ACHK1J_004560"
     CDS             1811..2119
                     /gene="yaiY"
                     /locus_tag="ACHK1J_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO7913732.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2178..3272)
                     /gene="yaiW"
                     /locus_tag="ACHK1J_004561"
     CDS             complement(2178..3272)
                     /gene="yaiW"
                     /locus_tag="ACHK1J_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO7913733.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSRAPQPLKKGEKEIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3285..4505)
                     /gene="sbmA"
                     /locus_tag="ACHK1J_004562"
     CDS             complement(3285..4505)
                     /gene="sbmA"
                     /locus_tag="ACHK1J_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO7913734.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4857..6014
                     /gene="ampH"
                     /locus_tag="ACHK1J_004563"
     CDS             4857..6014
                     /gene="ampH"
                     /locus_tag="ACHK1J_004563"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO7913735.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6015..6638)
                     /gene="iprA"
                     /locus_tag="ACHK1J_004564"
     CDS             complement(6015..6638)
                     /gene="iprA"
                     /locus_tag="ACHK1J_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO7913736.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1690 a         1711 c         1615 g         1653 t
ORIGIN      
        1 aaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc gttcatcatt
       61 tttgagatct tacgcacatc tttgagataa cgcactttct tcaccagtac gttgcacgct
      121 ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt tttgtcgtgg
      181 taatgtatgc actttatgtt agacgggata ggtttttaag atggaaaaac tgcgggtagg
      241 aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg caaaaaacat
      301 tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg ataaacaagg
      361 gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc ctgcccatat
      421 tgcgttgcgc ccttcggcga ccagcctcgc ccaggtgcca ggtaaacatg agcatcagct
      481 tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc cgattgtcca
      541 cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca atttaccgtt
      601 tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca ccaaacgtct
      661 gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg ctaatcgtca
      721 caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg taaaaccggc
      781 taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt acgcaattgc
      841 cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga tcaaaggtcg
      901 tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct gtggcgagat
      961 cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag atggcgcgaa
     1021 agtggtagtt ccggcagcca ttgcgccaga aatcaacgat aagatccggg cgattgccgt
     1081 tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt ttttaacccc
     1141 agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca acatcagtat
     1201 gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca cacgtttgat
     1261 tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa tgtaatattt
     1321 taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac gacgaataat
     1381 caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat aggtctgcca
     1441 accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata taaggccaat
     1501 ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca tggctgctcc
     1561 gttaacgaaa aacaacatcc ttgcatattc tgcaacaacc gtctgtggtg tagacaacat
     1621 tttcgcgata gttaataaat ttaacttaaa gttgctattt tgtgactcgc aacgaagatt
     1681 cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga caatctaaat
     1741 gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca gatgacttcc
     1801 ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa aatatcgcaa
     1861 tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt gcttttgggc
     1921 tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc gtttaatgca
     1981 ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca ccattttcgg
     2041 gctgatcccg tttttagtag gctgcctcat ttttgcagta gtggcgctat ggctgcactg
     2101 gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc cttatctggc
     2161 atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc
     2221 gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg aatgccaggc
     2281 aacatttcgc gggggagtga tttacccgtt ttcgcttcgg caagttgata aactttcttg
     2341 tacagcgccg tctcctcaaa agagaagctg tcacctttct ctagctgacg acgaatttcg
     2401 ctgtcgctca tccccagttt tgcagccagt ttgcgcgttg ccagttcggt tttcccgggt
     2461 tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc gctggcctta
     2521 ctgaccgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt aaaatcagca
     2581 aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta agtaccaaac
     2641 cacaacccgc cgcggcgact gaagacttcc tgacggactg taccgtccat tttccacgga
     2701 taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc accggtgcgc
     2761 accggattaa gcgaaccaaa cagcgtctgc cccattggca ccatgctgat cagatcgtca
     2821 aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct ataacttttg
     2881 ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aaggaggaat gtgcatccgt
     2941 tcagcacgac ggtcaatctc ttgccaggcg attttgctta agcccggaac ggccggatct
     3001 gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc
     3061 ggtgccagcc cctgactttc aaaggtggtg gcgagatctt tcgcccaggc atcgcggtct
     3121 ttcacgctcg caggcatctt ctgccgcacg acgctcgcca catctatctc tttttcgccc
     3181 tttttcaacg gttgcggagc ccgactacta caccccgcta aaaccagaac cgccaacaat
     3241 gacagcgaag atagaggatt gacgcgcgac attacttctc ctttttagct caaggtatgg
     3301 gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag acgtttgtag
     3361 atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa agcaccgcga
     3421 acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt accggcaaca
     3481 attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata gagaatgcga
     3541 gcgatgttga aatacatata gtgaaaatag aggcggaaat agtttttccg tacggcgcta
     3601 aacagctcgc gtaccgttgg cggcgtcgcg cgcgtggcat cgtcttcacc ataaaccagc
     3661 tctttacggt aggcagcctc tactcgctgg tttttaaact ccagccccgg cagtttgatc
     3721 cctaccactg ccagcaatcc ggtccccatc agcgaccaga cgatagcggc aatcaccaga
     3781 ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag cgttaccagc
     3841 accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacacc catattctcc
     3901 agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcgcc ttcgatatga
     3961 cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca gcggaacacg
     4021 tagtgactga caaagaagtt attcagcaca ctgatcacta ccgcgatcag cgcaatcccc
     4081 agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg cgaacttagc
     4141 gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcaac cccgacttcc
     4201 accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca gtattgccaa
     4261 cggtgcgggc tgtagataaa ccagaacaat gcaaaaagtc ctacgcaaac aatgtagtaa
     4321 gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg gatctgcccg
     4381 gaagcgccgg tgatacgcgc cacccagtca cccccaccgg cttgccagaa gataacggca
     4441 atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa aaaagactta
     4501 aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat taaccgcatg
     4561 accgctacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca aggcgcttag
     4621 ttcgccagcc tggccataag aattgtttcg acggatttca caaaccaaag attgtttaca
     4681 taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg aaaaagatcg
     4741 tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct ttgcaatcca
     4801 tcaatgagta gtataaatac gctcagttac cttcattcaa tttatggaca ccaccgttga
     4861 aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg
     4921 cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat gccgatcata
     4981 ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt aaccagcgcg
     5041 tctttcgcag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag ctggattcgg
     5101 tcgtgcgtat tgcttccctc accaagctaa tgaccagtga aatgctggtg aaattgctcg
     5161 accaggggac cgtgaagtta aacgatccgc taagtaaata tgctccgcca ggcgcacgcg
     5221 tgccaaccta caatgggacg cccattacgc tggtcaatct ggcaacccat accagcgccc
     5281 tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg ccaacgcgcg
     5341 agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc agtcaggcgg
     5401 cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg tccggcaagc
     5461 cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa gacaccacct
     5521 acaccccctc accggatcag tgccgccgtt taatggttgc agagcgcggt gccagtccat
     5581 gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct ggcgatatga
     5641 tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac caggcagatc
     5701 gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc atggatgtcc
     5761 ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa gagggtcgtc
     5821 cggggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg gcgatgatcc
     5881 cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg cgctttaaaa
     5941 atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa ccgttggtta
     6001 tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag tttgccttta
     6061 ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac gacagaacgc
     6121 gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg caattcttca
     6181 ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt atgcccaatg
     6241 agctgcacgt cgcgtaattc cagaattcta ttttgccagg ctaaccagta aaaagcgtct
     6301 cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag atgatatccc
     6361 gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa accatcagcc
     6421 agccccataa tataaggagc ctgggtaata ccgataagta cgttttcttc tctacgcaga
     6481 gagataacgc cctctaaaat aacgaaagta tcttcgttat tgacatcact ggaaaatata
     6541 acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca tttatcgagc
     6601 ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt tgcagtaatg
     6661 cccgtcaaa
//
LOCUS       DAZGBC010000215         6668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGBC010000215 DAZGBC010000000
VERSION     DAZGBC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.216"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            357..1271
                     /gene="shuT"
                     /locus_tag="ACHK1J_004565"
     CDS             357..1271
                     /gene="shuT"
                     /locus_tag="ACHK1J_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO7913737.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVAPHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRITDVVESLHQQLWPQ"
     gene            1291..2628
                     /gene="hutW"
                     /locus_tag="ACHK1J_004566"
     CDS             1291..2628
                     /gene="hutW"
                     /locus_tag="ACHK1J_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO7913738.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            2641..3135
                     /gene="hutX"
                     /locus_tag="ACHK1J_004567"
     CDS             2641..3135
                     /gene="hutX"
                     /locus_tag="ACHK1J_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO7913739.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPFSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            3135..3758
                     /gene="chuY"
                     /locus_tag="ACHK1J_004568"
     CDS             3135..3758
                     /gene="chuY"
                     /locus_tag="ACHK1J_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO7913740.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3807..4799
                     /gene="shuU"
                     /locus_tag="ACHK1J_004569"
                     /gene_synonym="chuU"
     CDS             3807..4799
                     /gene="shuU"
                     /locus_tag="ACHK1J_004569"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO7913741.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4796..5566
                     /locus_tag="ACHK1J_004570"
     CDS             4796..5566
                     /locus_tag="ACHK1J_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO7913742.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5618..6265)
                     /gene="yhiD"
                     /locus_tag="ACHK1J_004571"
     CDS             complement(5618..6265)
                     /gene="yhiD"
                     /locus_tag="ACHK1J_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO7913743.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQIRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6329..6655)
                     /gene="hdeB"
                     /locus_tag="ACHK1J_004572"
     CDS             complement(6329..6655)
                     /gene="hdeB"
                     /locus_tag="ACHK1J_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO7913744.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
BASE COUNT         1668 a         1690 c         1627 g         1683 t
ORIGIN      
        1 caagtttttt ttaaccacca ttaacacaat aaacaggggg ttatgaaacc tatcctgcgc
       61 cctgtaaacc accttgtttt tatggttatc cccccatgcc tcatcactaa attttgtcgt
      121 taacagtgat aattttttct tctctttgct ctgcaagcct cgatatctct gggttctcag
      181 ctttttcccg cgatgaaatt tagtcggtaa caagaacgtt agctgacgaa gcatcttgat
      241 aaaaatcaaa aataaatacg cttgtaacgc cttgcgtatt gaatcgacgg ttgtatttca
      301 tatataactc aaatgataat catgatcatt ctcaatttga taagagttct catattatgc
      361 caaggatcat caccaggccg tttctttttt ctccactgac gttatgtatt tcagccgtgg
      421 cctctgccgc gaagaccgca gtaaaacgaa aaaaattgtt tacggcagta ctggctctta
      481 gctgggcttt tagcgtaacg gctgccgaac gtatcgtggt cgcaggagga tcgctgacgg
      541 agctaatcta cgcgatgggc gctggcgaac gcgtggttgg tgtcgatgaa acgacatctt
      601 atccaccaga aaccgccaaa ctgccgcata ttggctactg gaaacaactc agcagcgaag
      661 gcattttgtc acttcgcccg gatagcgtaa ttacctggca ggatgccggg ccacaaattg
      721 tgctcgacca actacgagcg cagaaagtca acgtcgtcac cctgccgcgt gtgccagcca
      781 cccttgagca gatgtacgcc aacattcgcc aactggcaaa aacgctacag gttcctgaac
      841 aaggcgaggc gctggtgaca caaatcagcc aacgcctgga gcgagtacag caaaacgtgg
      901 cggccaaaaa agccccggtt aaagccatgt ttattctctc cgcaggcgga agtgcgccac
      961 aagttgccgg taaaggcagc gtcgcggacg ccattctttc gcttgccgga gcagaaaacg
     1021 tcgctcccca ccagcagtac aaaagctaca gtgcagaatc actgattgcg gctaaccctg
     1081 aagtgattgt cgtaacctca caaatggtcg atggcgatat taatcggcta cgttctattg
     1141 ctggaattac ccacactgcc gcatggaaaa accagcgcat tatcaccgtt gatcaaaacc
     1201 tgattctggg aatgggtccg cgtattactg atgtcgttga gtctttacac cagcagcttt
     1261 ggccgcaata aatggattta aatcagacga atgaacgcaa ataacaccct ggatctcacg
     1321 ccacattttg ctttagacgg tgatcagcca tttaaagaca ggcgcgccat gatgccgttt
     1381 cgtggggcca ttccagttgc caaagagcaa ctggcacaga cctggcaaga gatgatcaat
     1441 caaacggcat cacctcgtaa gcgactggtt tacctacaca ttcctttttg tgcgacacac
     1501 tgcacgtttt gtggttttta tcagaaccgt tttaatgaag atgcgtgtgc tcattatacc
     1561 gacgccctga ttcgtgaaat agaaatggaa gcggacagcg tattgcatca gtctgccccc
     1621 atccacgcgg tctatttcgg tggcggtacg ccttccgcac tttcggcaca cgatttggcc
     1681 agaatcatca ccacattacg agaaaagctg ccactggccc ctgattgtga aatcactatc
     1741 gaaggacggg tattgaattt tgacgctgag cgaatcgatg cttgccttga tgcaggagca
     1801 aaccgcttct caattggtat ccagtcgttt aacagcaaaa tccgcaagaa aatggcccgc
     1861 acctcggatg gcccaaccgc tattgcgttt atggaaagcc tggtcaaacg tgaccgtgct
     1921 gcggtggtct gtgacctgct ttttggcctg ccgggtcagg atgcgcaaac ctggggagaa
     1981 gatctggcta ttgcccgcga tatcggtctc gatggcgtcg atctctatgc gctcaatgtc
     2041 ctgcccaata caccgctggg caaagccgtg gaaaatgggc gtactaccgt gccctctccg
     2101 gcagaacgtc gcgatcttta tctgcaaggg tgtgacttca tggacgaagc cggttggcgc
     2161 tgtattagta acagccactg gggccgtacc acacgcgaac gcaatctcta taacctgctg
     2221 ataaaacaag gtgccgattg tctggccttt ggttccggag ccggcggttc gattaatggt
     2281 tactcctgga tgaacgaacg caatcttcag acctggcatg aatccgtcgc ggcaggcaaa
     2341 aaaccgctga tgatgatcat gcgtaacgcc gaacgtaatg cgcaatggcg tcataccttg
     2401 cagtcaggtg ttgaaacagc gcgtgtaccg ctggatgaac ttacgccaca tgccgaaaaa
     2461 ctcgcgccgt tactggctca atggcaccaa aaaggcttaa gtcgcgatgc ttcaacttgc
     2521 ctgcggctga ctaatgaagg tcgtttctgg gcaagcaata ttttgcagtc tcttaatgaa
     2581 ctaattcagg tacttaatgc gccagcgatt gtgcgtgaaa aaccataaca ggagtgattt
     2641 atgagccatg tctcgttaca agaatttttg aaaacggagc cggacggtac gctggaggtg
     2701 gtagccgaac aatacaacac cacactgctg gaggtagtga gaaatctacc cttctctacc
     2761 gtagtgccag gcgataaatt cgatactgtc tgggataccg tctgcgaatg gggcaacgtc
     2821 accacgttag tacatactgc cgatgtaatc ctcgaattta gcggcgaact gccttcgggt
     2881 ttccatcgcc acggctattt caacctacgc ggaaagcacg gcatgtccgg gcatatcaaa
     2941 gcagaaaact gcacgcatat tgccttaatc gaacgtaaat ttatgggcat ggacaccgcg
     3001 tccattctct ttttcaacaa agaaggcagc gccatgctga aaatcttcct cggcagggat
     3061 gaccatcgtc agctcctgag cgaacaagtc agcgccttcc atactctggc ggcatcgtta
     3121 aaggaacacg cctgatgacg ccgtggctac tctttggcgc aggcggaaaa ggtgtgggtg
     3181 ccagaacgct tgaactggcg ctggcggaac aacgtccggt cgttgctgtc attcgtcatg
     3241 ccgatgccgc cacgaaactg gcgcaacaag gcgtacaggt ttttacaggc gacgcctgtg
     3301 atgccagcgt agtcgcggct gcttgccgcg cagctggccc ggatgcgctt atcatctcaa
     3361 caatgggagg agctcaggat tatctggcgc acagaacagt gattgatgag gccgaaaagg
     3421 ccggcatcag tcgcatgatt ctggtgacct ctttgggatg cggcgatagc tggccgtttt
     3481 tatcggagcg ggcaaaggct gcgtttggtc aggcggtacg ggaaaaaacg ctggcggaaa
     3541 gctggctgca aaccagccag ctagactacg ccattctccg ccctggtggt ctgcttgacg
     3601 gcgcagcaac aggcaaagca caacgggtac aaaatcagga gtgtcatggt tttgtccgcc
     3661 gtgccgatgt cgccgcacat atccatgaac tggcaaatgc gccagcactt aaccagcagg
     3721 tctacagtct gattgaaccg gacctgaaac cggcgtgata ccccggcgcg gcatgtccgc
     3781 gccgtaatgt cgtaacggag ttttgcgtgc tcaaggattc attttcatcc gccagggtct
     3841 ttatggggct atcactatta ttgcttgccc tggtgctgtt cggtgccagt cagggagcgt
     3901 taaagatcag ttttgatgcc ctttttgatg aggaataccg cgatatctgg ctcaatattc
     3961 gtctaccacg ggttttgctg gcggtgctgg taggtgcagc gttagcaacc gcaggggtaa
     4021 ttatgcaggg gctttttcgc aacccaatgg ctgacccagg attacttggc gtcagtagcg
     4081 gttctgcatt aatggttggc gttgctattg tgctgccctt ctccttccca gtggttctgg
     4141 tgctctatga gcaaatggtg tttgccattg ccggaagttt agtagtctgc accatcattt
     4201 ttctcatcac gcagcgccat cgcgatggca gcatgatgca attattactc gccggtatcg
     4261 ccatcaatgc cctgtgcggc gcagcgatcg gcatcctgag ctatatcggc gatgagcagc
     4321 agctacgaca actcacattg tggatgatgg gcaatcttgg acaggcgcaa tggccgacgt
     4381 tattggttgc cagttcattc atccttccgg ccattatcgc aacaacttgt ctcgccggaa
     4441 cgctgaattt actgcagctc ggtgatgaag aagcccatta cctcggcgtg aacgttaagc
     4501 gtaaacgcca gcaattactg ttagtgagct cactgctcgt tggtgctgcc gtatcggtaa
     4561 gcggcattat cggctttatt ggcctggtga tcccgcatct gattcgcatg actaccggag
     4621 caaatcaccg ctggctaatc ccttgttccg ccctcgccgg agcctgttta ttgctgatgg
     4681 cagacacgct tgcccgcacg ctggtacagc cagcagaaat gcccgtggga ttattaacca
     4741 gcctgcttgg tggcccttat tttatgtggt tgattctgcg aaatcggagg atcacatgat
     4801 cagcgcccag aatctggttt atagccttca ggggcggcgt ttgactgata atgtctcgct
     4861 gacttttccc ggtggggaaa ttgttgccat tctcggccct aatggtgcgg gaaaatctac
     4921 gctgcttcgc cagttaaccg gctatcttca acctgatagc ggcgaatgtc ggttgtttaa
     4981 taagccatta aatgaatggt cgattactga actggcgaaa caccgggccg tgatgcgtca
     5041 aaatagccac atggcgtttc cattcagcgt acaagaagtt atccagatgg ggcggcatcc
     5101 gcatcgtact ggtaatcagg ataatgaaac agcacaaata atggcgttat gtgactgtca
     5161 ggcgttagca aaccgcgact atcggcaatt atccggtggt gaacaacagc gcgtgcaact
     5221 agcgcgcctg ctggtgcaac tctgggaacc tactccctca ccgaaatggt tatttcttga
     5281 tgaaccaact tcggcgctgg atattcacca tcagcagcac ttgtttcgtt tattacgcca
     5341 gttagttcac gagcggcagt tcaatgtctg ctgcatattg cacgatctaa atcttgccgc
     5401 gcgttatgca gaccgtgttg tattaatgca aaaaggaaag gttatcgcaa atggtaaacc
     5461 acaggacgta ttgacacagc aggcgctgac aatgctctac ggcgcggata ttaccgtact
     5521 taaagatcct gccaaccatt caccactgat cgtcctcgat cattaaaagc aacaggagta
     5581 ccgcactggt actcctgcga ataaataact atacagatta gctaattgaa acgcctttca
     5641 cccctgccat accttttaat aatcgcaaca gatcttctat cgacgttgtg gcatgaagct
     5701 ggatatcaat ggcgaccact tcatgatctt cattttcctg caacgaaact aaatcagttt
     5761 ttattttttg ctgcttaaac cagtcgagca ttgataccgt attaccgttc actagtgtca
     5821 gctgtaaatg gtaatttttg ttcatcaggc ggaaggttaa ttgatggaag acttccagca
     5881 ccagcaaggt catcactgaa ccataaatac ccagttcgta cataccactg ccaataacca
     5941 taccaatggc ggcggtcacc cagatatccg ctgcagtcgt cagacctaca atattttggt
     6001 tacgaacgag aatgttacct gcaccgataa acccgacgcc cgtcaccacc tgagcagcaa
     6061 tacggctggg gtccagtccg acgtgatcta aagacagcac gtcagcaaaa ccatatttcg
     6121 aaacaatcat aaacagggcg cttcccatgc caattaatac atgtgtgcgt aaccctgctc
     6181 ctttgccgcg tatttgcctt tccatgccaa tagcgccaca ggcaattgcc gcgagtataa
     6241 gacgtataat aaattctgct gtcatataaa aatgcaaaaa ggagcagcaa gatggctcaa
     6301 cttgctactc ctttttactt gtacctcatt aattcggcaa gtcattagat gcttgatttt
     6361 tgaaggtata caaatttttc tgcgggtttt tcttacagta ttcgatcact ttaggaattt
     6421 gagtgagatc ggtttcattt aaagtaacgg tatcgccacc tttatatact gtttcttcat
     6481 gcagcatcca ccatgcaacc ggggtcattg cttttggatt cagatcaata aattcctggc
     6541 aggtcatatc tttagcggat tcattggctg ccagcgcaga ttgtgcgttc accagtgaca
     6601 aagctgctac agcgcccata aaaataaacg ctttacggag agatgaaata ttcattttgt
     6661 aacccatt
//
LOCUS       DAZGBC010000216         6652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGBC010000216 DAZGBC010000000
VERSION     DAZGBC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.217"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..237)
                     /locus_tag="ACHK1J_004573"
     CDS             complement(<1..237)
                     /locus_tag="ACHK1J_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HFO7913745.1"
                     /translation="MRSLFALLITVISCTTASAAMTETCQKYFDETDKFLSQMKTTGV
                     AEGQINLMKQQYEQSKKQFSSLPEASQDAACKQAS"
     gene            complement(991..1239)
                     /locus_tag="ACHK1J_004574"
     CDS             complement(991..1239)
                     /locus_tag="ACHK1J_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7913746.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIH"
     gene            complement(1465..1560)
                     /locus_tag="ACHK1J_004575"
                     /pseudo
     CDS             complement(1465..1560)
                     /locus_tag="ACHK1J_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1557..2123)
                     /gene="yecD"
                     /locus_tag="ACHK1J_004576"
     CDS             complement(1557..2123)
                     /gene="yecD"
                     /locus_tag="ACHK1J_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7913747.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLHRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2433..4205
                     /gene="aspS"
                     /locus_tag="ACHK1J_004577"
     CDS             2433..4205
                     /gene="aspS"
                     /locus_tag="ACHK1J_004577"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO7913748.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4323..4775
                     /gene="nudB"
                     /locus_tag="ACHK1J_004578"
     CDS             4323..4775
                     /gene="nudB"
                     /locus_tag="ACHK1J_004578"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO7913749.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4804..5544
                     /gene="yebC"
                     /locus_tag="ACHK1J_004579"
     CDS             4804..5544
                     /gene="yebC"
                     /locus_tag="ACHK1J_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7913750.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5579..6100
                     /gene="ruvC"
                     /locus_tag="ACHK1J_004580"
     CDS             5579..6100
                     /gene="ruvC"
                     /locus_tag="ACHK1J_004580"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO7913751.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6102..6650)
                     /gene="yebB"
                     /locus_tag="ACHK1J_004581"
     CDS             complement(6102..6650)
                     /gene="yebB"
                     /locus_tag="ACHK1J_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO7913752.1"
                     /translation="MGAALFGQISAASNCWSNHVGIIIGHNGEDFLVAESRVPLSTIT
                     TLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHTGFKYESSRQFCSK
                     FVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWERKTVTPASLWHH
                     PGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1686 a         1563 c         1726 g         1677 t
ORIGIN      
        1 acttgcttgt ttacaggcag catcctggct tgcctcgggc agagatgaga attgtttctt
       61 tgattgttca tattgttgtt tcattaaatt aatctgtcct tctgctactc cggttgtttt
      121 catttgcgat aagaacttat ctgtttcatc gaaatacttc tgacatgttt ctgtcatagc
      181 cgcactggca gttgtgcagg atattacagt gattaataaa gcaaagagac ttctcatata
      241 tcctccattg ttttgtttga aaaattaacg ctattaaata actcatagac aggtaaattt
      301 atgtgattat agtcataaac tataaaaata ttgatgttgt ctttgtagaa aggttgtgag
      361 caattatgaa aatttatttg cttgttcact ggttttaggg agtttgatgt gtccatgaag
      421 cgtttctgac gagaaatcaa cataaatcac cagttggtga actaggattg ccagaaacgc
      481 tcctgtcagt tctgtagtgt gtagtgctca cttagagcct ggtgaactgg ataatgagtc
      541 gcacctcagt atcctgcaat gggctgcttt tcccctgctc ctgattcttt tttgtttcag
      601 gtcttaccaa cataatggtt gttgtaataa attgcgtgtg attctgtatc gggtcaacca
      661 tgtgacgata catgttcagt aaggaagact gaagggttca gagtgcgagt atgattaata
      721 gatggaatca gcgtttcaaa gattttgagg gaatataagg tctgacctga tgattacctt
      781 ttgcgcctgt atcctttgca taaaggaggt tgatgtagat aaagtcatct ccacttgtga
      841 tataaaacag tcagttatga aaaatcggca taaacatcct tttgttaacc ttaaagccag
      901 acttacggtt tttccatagt tacagataag cggctagtaa ggttttacag gtgataagcc
      961 tgtaacggaa atcacctgca aatctattaa ttagtgaata aaccagtcat ccgctgtttc
     1021 ccagacttcc tgaagcgttt cttgtacaaa ctcttgcgcg gattctttat ccacggctct
     1081 taaaatagtg agtccatcat tgctggcaga tttaacgata acatctacat cgtcataacg
     1141 cttaccgaca cgtcgtagca tttcctgttg cagggcagga acagaacctt ttggcatttt
     1201 gctgattttt tctttagcga tacagatctc aacacgcata acaccctcct gtaactgtgg
     1261 ttatgtacag gtgttatttt tatctgtatg gataaccagt gtcaattcct gatgttgttt
     1321 atggggcatc aatggggcat gtatgggaca ctttttatcg gcgaaattcg tcgaagttcg
     1381 tcgacatggt aaacgaatca tctatccaac ccttgaaaaa cggcgctcct ggacgatctt
     1441 cgtcgatttt taaaaatgtt gcgtcacgca gttaaagtgg cgggcatact cttcaaggcg
     1501 ggtgatcccc aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat
     1561 aacgcgtgaa ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta
     1621 atgctgttat tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga
     1681 ttaaagccaa gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc
     1741 ccacataaca ctattgtatc gatacctcgg cggtgtaatt gcaactccag atccgtaccg
     1801 taaaacgcac cccactgacg tttgattatt tcgatatcac tgtcagttgc acctaatgca
     1861 gctggatgtt gccaccagtt ttcgggcaac actttagcgg gggaaggggc atcaaccggc
     1921 tgttttaatg cttcggcgta atcggcagac cagccaacgc gcaccagaaa cacgggctga
     1981 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
     2041 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac cagcgcagtg
     2101 gttttagcat taagttcaag catgatgact cctgaaaaaa caaaatcgtg cctcacacct
     2161 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
     2221 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
     2281 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
     2341 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
     2401 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
     2461 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
     2521 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
     2581 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
     2641 aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
     2701 gcgaaatcga agtgctggcg tcctcgctga ctatcatcaa tcgcgcagat gttctgccgc
     2761 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
     2821 gtcgcccgga aatggctcag cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
     2881 gttttatgga tgaccacggc ttcctcgata tcgaaactcc gatgctgacc aaagccacgc
     2941 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     3001 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggcttt gaccgctact
     3061 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     3121 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     3181 cgctggtgcg ccatctgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     3241 tgacctttgc ggaagctgaa cgccgttacg gttccgataa accagatttg cgtaacccga
     3301 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcaggtc
     3361 cggcgaacga tccgaaaggt cgcgttgccg ctctgcgtgt tccgggtggc gcatcgctga
     3421 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     3481 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     3541 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     3601 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgcgatg ggcgcactgc
     3661 gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     3721 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     3781 cgttcacctc accgaaagac atgacggctg cagagctgaa agctgcaccg gaaaatgcgg
     3841 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     3901 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     3961 agcgcgagaa attcggcttc ctgctcgacg ctctgaaata cggtactccg ccgcacgcag
     4021 gtctggcatt cggtcttgac cgtctgacca tgctgctgac cggcaccgac aatatccgtg
     4081 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     4141 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     4201 actgatatga gtcaaataca cgaaatcatt cgcgttgcat cgaggcggca actgagtgaa
     4261 ctcccatgag catagataac tatgtgaata ggatgagcga aggcagtcaa cgaagaggca
     4321 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tacgcacaag
     4381 atacgaaacg ggtgctgatg ttgcagcggc gtgatgaccc cgatttctgg cagtcggtaa
     4441 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     4501 aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggttg
     4561 agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg
     4621 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     4681 cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc
     4741 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     4801 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     4861 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     4921 ggtggcgatc cggacgctaa cccgcgtctg cgtgcagcgg tggataaagc actgtctaac
     4981 aacatgaccc gtgacactct gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     5041 gcgaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     5101 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcatgc attcagcaaa
     5161 tgtggcggta acctcggtac tgatggttcc gtagcctatc tgttcagcaa aaaaggcgtc
     5221 atctccttcg agaaaggcga tgaagacacc atcatggaag cagcactgga agcaggtgct
     5281 gaagacgttg tgacctatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     5341 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     5401 atgatcccgt ctaccaaagc tgatatggat gcagaaaccg caccgaaact gatgcgtctg
     5461 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     5521 gatgaggtcg cagcgactct ctgatgaggc ctgctaaaca gcaaaacgga gacgcgtgat
     5581 ggctattatt ctcggcattg atccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     5641 ggtaggtagg caactgtcct acctgggtag cgggtgcatc cgcaccaaag tggatgattt
     5701 accgtctcgt ctgaaactca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     5761 tgattatttc gccattgaac aagtctttat ggcaaagaac gctgactcag ccctgaaact
     5821 gggccaggcg cgcggcgtgg cgattgtggc ggcggtgaat caggagttgc cagtatttga
     5881 atacgcggca cgtcaggtaa agcaaacggt ggtaggtatt ggtagtgccg aaaaaagcca
     5941 ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg cggatgccgc
     6001 cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga tgcagatgag
     6061 cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc ctcggtcagt
     6121 tccggctgag gcgtttccac tccctccgca tgaatcaaca ccaaacccgg atgatgccac
     6181 aaactggctg gcgtgacggt tttacgctcc cacggaatag aacccaggaa ccagaatttc
     6241 cagaaagtga gttttgcatt cggattgcta tttaacaatt ctccaaacgt ctctatttca
     6301 cccaccggaa tacatagcgc ttctttataa atatcaaaaa caaattttga acagaactgg
     6361 cgcgaagatt cgtatttaaa accggtgtgg taaagtttgc gtagccggga gggaacctgt
     6421 tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt tatagcatag
     6481 cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga aaggggaacc
     6541 cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc gacgtgatta
     6601 ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat ac
//
LOCUS       DAZGBC010000217         6612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGBC010000217 DAZGBC010000000
VERSION     DAZGBC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.218"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1170
                     /locus_tag="ACHK1J_004582"
     CDS             <1..1170
                     /locus_tag="ACHK1J_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO7913753.1"
                     /translation="SYKPIWNWKISQYNVSDIKFEKIIKPQFADRTYFSNCSPVDPTS
                     TRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQ
                     LDTNVNTIISILNRRDIILESLQPYLVINKGAVTPCTFLGDQTGDRFSNICGDQFIID
                     LLKCIMSINDNVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDSKYK
                     IMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHTIINSKKFKTS
                     RAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGL
                     NARDSKHTGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1360..1473
                     /gene="yhiY"
                     /locus_tag="ACHK1J_004583"
     CDS             1360..1473
                     /gene="yhiY"
                     /locus_tag="ACHK1J_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO7913754.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1536..2603
                     /gene="yhiI"
                     /locus_tag="ACHK1J_004584"
     CDS             1536..2603
                     /gene="yhiI"
                     /locus_tag="ACHK1J_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7913755.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2600..5335
                     /gene="rbbA"
                     /locus_tag="ACHK1J_004585"
     CDS             2600..5335
                     /gene="rbbA"
                     /locus_tag="ACHK1J_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO7913756.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5335..6459
                     /gene="yhhJ"
                     /locus_tag="ACHK1J_004586"
     CDS             5335..6459
                     /gene="yhhJ"
                     /locus_tag="ACHK1J_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7913757.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1564 a         1637 c         1819 g         1592 t
ORIGIN      
        1 tcgtacaaac ccatctggaa ctggaaaata tcgcaataca atgtatctga tattaaattc
       61 gaaaaaatta taaaacccca gttcgctgac agaacctatt tctccaattg ttcacctgtt
      121 gatcccacaa gtaccagacc aacctatttt ggtgataccg atggctcagt tggtgcagtt
      181 ttgtacgcgc tttttgcaac gggacatttg ggaatcatgg ctgaaggaga aaatttcctt
      241 agccagttgc ttaatataga agatgaagtt ctcaatgtat tgctaaggga gaactttaat
      301 gagcaacttg atactaatgt taatacaata atcagtatcc tgaacagaag agacattatt
      361 ttagaaagtc ttcagcctta tttagttatt aataaaggcg cagtgacgcc atgtacgttt
      421 ttgggtgacc aaaccggcga tcgatttagt aatatctgtg gcgatcaatt tatcatcgat
      481 ttgttaaaat gcataatgag catcaatgat aacgttcatg tattagctgg aaatcatgaa
      541 acgaattgca atggtaacta tatgcagaac ttcacgcgta tgaaaccact tgatgaggat
      601 acctacgctg ggataaaaga ttatcctgta tgtttctatg actccaaata caaaataatg
      661 gcaaatcatc atgggataac ttttgatgat cagcgaaaac gttacattat aggaccgata
      721 acggtctcta ttgatgaaat gaccaatgct ctcgatccgg tcgaattagc ggcaattatt
      781 aataaaaagc atcatactat aattaatagt aaaaagttta aaactagccg ggctatctct
      841 tgtcgatcct ttaatcgtta tttttctgtt tcgacagatt acagacctaa acttgaagca
      901 ctattagcat gctcacaaat gttaggtata aatcaagttg ttgcacataa cggtaatggt
      961 ggaagagaac gcataggtga aacggggaca gttttaggtc ttaacgcacg tgacagtaaa
     1021 catacgggaa gaatgttcag catgcataat tgccaaatta accctggtgc ggggcctgag
     1081 ataaccactc cctggaagtc ttaccaacat gaaaaaaaca gaaatggact tatgcctttg
     1141 attagaaggc gcacaatgtt acagctctga accatagaca acatgaatat taatgttact
     1201 atctggttga gactgttgat aaaacgtaat aaggaatgct ctgttcctgc aactgaacag
     1261 gcgtgaaacg ctatccaaca ggatggatac cgttttgtca gccagtttta tagatcacac
     1321 ttatgagtat gcgtgtttca tttacggcca ggaatgctga tgatgacaac attactaccc
     1381 gttttcacta agccttcccc gttggcgctc aatgctctgc gcgctggtcg tatttgtcgt
     1441 ttccttctta tcccggatgg aagaatccgc tgaacgtcgt tcggcggcag ttgtaacacg
     1501 cgtcgtttgc ttacagggat tatggcgggt cgattatgga taagagtaag cgccatctgg
     1561 cgtggtgggg tgtcgggtta ctggcggtgg ctgctatcgt ggcgtggtgg ctgttgcgcc
     1621 cggcaggtgt gccggaaggc tttgccgtca gcaatgggcg cattgaagcg acggaagtgg
     1681 atatcgccag caaaattgcc gggcgtatcg acaccattct ggtgaaagaa ggccagtttg
     1741 ttcgcgaagg cgaagtgctg gcgaagatgg atactcgcgt gttgcaggag cagcgactgg
     1801 aagccatcgc gcaaatcaaa gaggcacaaa gcgccgttgc cgccgcgcag gctttgctgg
     1861 agcaacgaca aagcgaaact cgcgccgcac agtcgctggt taatcaacgc caggccgaac
     1921 tggactccgt tgccaaacgt catacgcgtt cccgctcgct cgcccaacga ggggctattt
     1981 ctgcgcaaca gctggatgac gatcgcgccg ccgctgagag cgcccgagct gcgctggaat
     2041 cagcaaaagc ccaggtttcg gcttctaaag ccgctataga agcggcacgc accaatatca
     2101 ttcaggcgca aacccgcgtc gaagccgcac aagccactga acggcgcatt gccgcagata
     2161 tcgatgacag cgaactgaaa gccccgcgtg acggacgcgt gcagtatcgg gttgccgagc
     2221 caggggaagt gctggcggca ggcggtcggg tgctgaatat ggtcgatctc agcgacgtct
     2281 atatgacctt cttcctgcca accgaacagg cgggcacgct gaaacttggc ggtgaagccc
     2341 ggctgatcct cgatgccgcg ccagatctgc gtattcctgc aaccatcagt tttgtcgcca
     2401 gtgtcgccca gttcacgcca aaaaccgtcg aaaccagcga tgaacggctg aaactgatgt
     2461 tccgcgtcaa agcgcgtatc ccaccggaat tactccagca gcatctggaa tatgtcaaaa
     2521 cgggcttgcc gggcgtagcg tgggtgcggg tgaatgaaga acttccgtgg cctgacgacc
     2581 tcgtggtgag gttgccgcaa tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca
     2641 actggcgggc gtgagccagc attatggaaa aaccgttgcg ctgaacaata tcacactcga
     2701 tattccggcc cgctgtatgg tcgggctgat tggtccggat ggcgtcggga agtcgagttt
     2761 attgtcattg atttccggtg ctcgtgtcat tgagcagggc aatgtgatgg tgctgggcgg
     2821 cgatatgcgc gacccgaagc atcgccgtga cgtttgcccg cgcatcgcct ggatgccgca
     2881 ggggctgggc aaaaatctct accacacctt atcggtgtat gaaaacgtcg attttttcgc
     2941 tcgcctgttc ggtcacgaca aagcggagcg ggaagtccga attaatgagc tgctgaccag
     3001 caccgggtta gcaccgtttc gcgatcgtcc ggcagggaaa ctctccggcg ggatgaagca
     3061 aaaactgggg ctgtgctgtg cgttaatcca cgacccggaa ctgttgattc ttgatgagcc
     3121 aacaacgggg gttgacccgc tctcccgcgc ccagttctgg gatctgatcg acagtattcg
     3181 ccagcggcag agcaatatga gcgtgctggt cgccaccgcc tacatggaag aggccgaacg
     3241 cttcgactgg ctggtagcga tgaatgccgg agaagtgctg gcaaccggca gcgccgaaga
     3301 gctgcggcag caaacgcaaa gtgccacgct ggaagaggcg tttataaatc tgttaccgca
     3361 agcgcaacgc caggcgcatc aggcggtagt gatcccacca tatcaacctg aaaacgcaga
     3421 gattgccatc gaagcgcgcg atctgaccat gcgttttggt tccttcgttg ccgttgatca
     3481 cgttaatttc cgcattccac gcggggagat ttttggtttt cttggttcga acggctgcgg
     3541 taaatccacc accatgaaaa tgctcaccgg actgctgccc gccagcgaag gtgaggcgtg
     3601 gctgtttggg caaccggttg atccaaaaga tatcgatacc cgccgtcggg tgggctatat
     3661 gtcgcaggcg ttttcgctct ataacgaact caccgtgcgg caaaacctgg agttacatgc
     3721 ccgtttgttt cacatcccgg aagcggaaat tcccgcgcga gtggctgaaa tgagcgagcg
     3781 ttttaagctc aacgacgttg aagatgttct gccggagtca ttgccgctcg gcattcgcca
     3841 gcggctttcg ctggcggtgg cggtgattca tcgcccggaa atgttaatcc tcgatgagcc
     3901 tacttctggc gtcgatccgg tggcgaggga tatgttctgg cagttgatgg tcgatctctc
     3961 gcgccaggac aaagtgacca ttttcatctc cacccacttt atgaacgaag cggaacgttg
     4021 cgaccgtatc tcactgatgc acgccggaaa agtgctcgcc agcggtacac cgcaggaact
     4081 ggttgagaaa cgcggagccg ccagtctgga agaggcattt atcgcctatt tgcaggaagc
     4141 ggcaggacag agcaacgaag ccgaagcgcc tcccgtgata cacgatgcta cacacgcgcc
     4201 gcgccaggga tttagcctgc gccgtctgtt tagctacagc cggcgcgaag cactggaatt
     4261 gcgccgcgat ccggtccgtt cgacgctggc gctgatggga acggtgatcc tgatgctgat
     4321 aatgggttac ggcatcagta tggatgtgga aaacctgcgc tttgcggtgc tcgaccgcga
     4381 ccagaccgtc agtagtcagg cgtggacgct caacctctcc ggttcccgtt actttatcga
     4441 acagccgccg cttaccagtt atgacgagct tgatcgtcgg atgcgtgcgg gcgatatcac
     4501 ggtggcaatt gagatccctc caaatttcgg gcgtgatatc gcgcgtggta cgcctgtgga
     4561 actcggcgtc tggatcgacg gagcgatgcc cagtcgtgcc gaaacggtaa aaggttacgt
     4621 gcaggctatg caccagagct ggctacagga tgtggcgagc cgacaatcca caccctccag
     4681 ccaaagcggg ctgatgaaca ttgagacgcg ctatcgctat aacccggacg taaaaagcct
     4741 gccagcgatt gttccggcgg tgatcccgct tctgctgatg atgatcccgt caatgctaag
     4801 cgcccttagt gtggtgcggg aaaaagaact agggtcgatt atcaaccttt acgtgactcc
     4861 caccacccgt agcgaatttt tgcttggcaa acagctaccg tacatcgcgc tggggatgct
     4921 gaactttttc ctgctctgcg gtctgtcggt gtttgtgttt ggcgtaccgc ataaaggcag
     4981 tttcctgacg ctcaccctgg cggcgctgct gtatatcatc atcgccaccg gaatggggct
     5041 gctgatctcc acctttatga aaagccagat tgccgccatt ttcggtacgg cgattatcac
     5101 gttaatcccg gcgacacagt tttccgggat gatcgatccg gtagcttcgc tggaaggtcc
     5161 aggacgttgg atcggcgagg tttacccgac cagtcatttt ctgactatcg cccgcggaac
     5221 gttctcgaaa gcgttggatc tgactgattt atggcaactt tttatcccgt tgctgatagc
     5281 cataccgctg gtgatgggct taagcatcct gctgctgaaa aaacaggagg gatgatgcgc
     5341 catttacgca atatttttaa tctgggtatc aaagagttgc gcagtctact cggtgataaa
     5401 gcgatgctga cattgattgt cttctcgttt acggtgtcgg tgtattcgtc agcgaccgtt
     5461 acgccaggat cgttgaacct ggcaccgatc gccattgccg atatggatca atcgcagtta
     5521 tcgaatcgga tcgttaacag cttctatcgc ccgtggtttt tgccaccgga gatgatcacc
     5581 gccgatgaga tggatgccgg actggacgcc ggacgctata ccttcgcgat aaatattccg
     5641 cctaattttc agcgtgatgt cctcgctgga cgccagccag atattcaggt gaacgtcgat
     5701 gccacgcgca tgagccaggc atttaccggc aacgggtata tccagaatat tatcaacggt
     5761 gaagtgaaca gctttgtcgc gcgctaccgt gataatagcg agccgttggt atcgctggaa
     5821 acccggatgc gctttaaccc gaacctcgat cccgcgtggt ttggcggggt gatggcgatc
     5881 atcaacaaca ttactatgct ggcgattgta ttgaccggat cggcgctgat ccgcgagcgt
     5941 gaacacggca cggtggaaca cttactggtg atgccgataa cgccgtttga gatcatgatg
     6001 gcgaaggtct ggtcgatggg gctagtggtg ctggtggtat cgggattatc gctggtgctg
     6061 atggtgaaag gcgtgctggg cgtaccgatt gaaggctcga tcccgctgtt tatgctgggc
     6121 gtggcgctca gtctgtttgc caccacgtca atcggcattt ttatggggac gatagcgcgt
     6181 tcaatgccgc aactggggct gctggtgatt ctggtgctgc tgccgctgca aatgctttcc
     6241 ggtggatcca cgccgcgcga aagtatgccg cagatggtgc aggacattat gctgaccatg
     6301 ccgacgacac actttgttag cctcgcgcag gccattctct accggggtgc cggattcgaa
     6361 atcgtctggc cgcagttcct gacgctgatg gcaattggcg gcgcgttttt caccattgcg
     6421 ctgctgcgat tcaggaagac gattgggaca atggcgtaaa tacaattgcc cgatgcgtaa
     6481 gcttataggg actacgcatc ggtgcaattt attgaatttt agttgttttt aggccggata
     6541 aggcgtttat gaaatgaccc ccaaaacctg gacagtttca gttaaccggc tttttgatac
     6601 tcagtccggt at
//
LOCUS       DAZGBC010000218         6520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGBC010000218 DAZGBC010000000
VERSION     DAZGBC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.219"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHK1J_004587"
     CDS             <1..129
                     /locus_tag="ACHK1J_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7913758.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHNLHVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHK1J_004588"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHK1J_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7913759.1"
                     /translation="MERESQPARTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPDEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPSAEDGHYRFE"
     gene            1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHK1J_004589"
     CDS             1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHK1J_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7913760.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGMISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDAAGYQMLLRRADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            1744..4296
                     /locus_tag="ACHK1J_004590"
     CDS             1744..4296
                     /locus_tag="ACHK1J_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7913761.1"
                     /translation="MDNRSAFFDGQPRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LEYTRQCAVFQNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFI
                     QRILSEVGIFWRTEMDDVRGLDTYIFADSQLNYQFDVCLPYSEPSGLFDGAAESVWDV
                     RTWHNVAIGTVSTRDYNYRTASMPMDATVSVRNDAVTTGEHYRDAPAYREAGDDTSPE
                     PETESGAFYARLHHERELNRSARIHLFSNAAHLSPGQVLEPQGDVIADLKEGIVLTLV
                     TYRGARDSRLHVSAWGMPYTERYCFRPAEIPRPVIPGTLPARIESRGKNDIYAHLDGQ
                     GRYRVKMDFDREETEPGFGYLWLRMAKPCAGETLGWHTPLIDGTEVAIAYSNGDIDLP
                     YIAYALHDAEHPDLVSRDNHTRNILRTPANNKLRMEDRRNEEHIKLATEYGKTQLNSG
                     HLVDSQGQLRGKGTELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLN
                     QQLQQLENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALTSGEDVQLAA
                     GQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQAQNGSMRLF
                     AEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKVKRTIAAGAA
                     SQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGNLDGAGYLHL
                     EDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSEGVE"
     gene            4293..5837
                     /locus_tag="ACHK1J_004591"
     CDS             4293..5837
                     /locus_tag="ACHK1J_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913762.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            5834..>6520
                     /locus_tag="ACHK1J_004592"
     CDS             5834..>6520
                     /locus_tag="ACHK1J_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFO7913763.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHGDYLMYPPHNNVCNFF
                     DAAYLLELDNYKYNFNKLAYPVFPENLPPVPERSDIIIKTDDPVPCDGIWEPVKIHYD
                     HKLLIIKDGIS"
BASE COUNT         1572 a         1559 c         1981 g         1408 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctac gctgatgtcc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacaa cctgcacgta
      121 ccgggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcaggccct ggcccgggat gggattccct gtcagcaccc
      301 tgaaagcggt ggagatagac gaagaccgtc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgccgtga aggacatgag gcgctcacct catttctgga tattttcagc caccgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtatcc ggccactttt gaggaggggg
      541 gacgcgatgc cacatcgcag tgtctgctgg ggctggtcgg actcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt ttctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgcggaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac tgaacccgat ccggttaagg tccatatcgg taaccgcagt ggactgggtg
      781 ccggaaaccg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgga cgaggcggaa ggctggttgc
      901 ccggaggtgt tctccatacc gatttgctgg tcctgctgcg cgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgcctt actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttactggg gctgaaagcg ggcaaactca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggttgcta cgaagggctg aaatgtgctg
     1141 cgctaccgtc tgctgaagac gggcattatc gcttcgaata atcacctgtt ctctctcaat
     1201 aaaggaacgc ctgaatggta acagtaatcc gctggtgggc gccgatgttt attgcctgtg
     1261 gcctgctcgc aggctgcggt ctgacgcaga ccgtcacgga tggtatgatc agcatcacga
     1321 aatccatctt ctacaaaaaa atcaaaacgc tgcatctgga ttttaccccg cgtacggcca
     1381 ttaacactga tggggcacag acgccactgg caaccatggt gcgggtttat cagctcaggg
     1441 atcgtaaagc ggttgatgcc gccggttacc agatgctgct gcgccgggcc gatacggtac
     1501 ttaaggacga cgtgctggcc tcaaaagaac tgctggtgat gcccggcggc agcgtgacgc
     1561 tcaatatgcc catggatgaa gatgcgcagt ttgtggctgt ggtgggactg tttaaccggc
     1621 cggatcagaa ggacaatcgc tggcgcctgg tactgacccg tgatgacctc aatccggata
     1681 agccccgcac cattgaactg ggtgatggct ggctcagtct tgtgccggta aaggagtgac
     1741 gtgatggata accggtcagc gttttttgac ggacagcccc gttactttct ggaaattaat
     1801 ggcagtgcag taaagcctga tgttttacgt tttcgtggca gggaagcact gagtgagccg
     1861 ttccgatggg acatcgggtt cacctcccgg aatgcgaaca ttcctccgga agaggtgctg
     1921 atgaaatatg cgtcgttgcg gatgcgtaac ggcaaaaatg tccatggcat tgtgacccgt
     1981 ctggaatggc tgtccacatc gaaagaccag tcccactacc ggcttacact cagctcccgt
     2041 ctggcactgc tggagtacac ccggcagtgt gccgtatttc agaaccagtc cgtccctgag
     2101 gtggtggagc aggtgctgcg caaacacggg ctggagggcg ctgattttga attcagactg
     2161 gaacgcgctt acccgtcgcg tgaaattatc acccagtggc aggaaacgga tttgcagttc
     2221 attcagcgca ttctgtctga ggtggggatt ttctggcgca cggagatgga tgatgtgcgc
     2281 ggactggata cgtacatttt tgccgacagc cagcttaact accagtttga tgtgtgtctg
     2341 ccgtacagcg agccgtcggg cctttttgac ggtgctgcag aatcggtctg ggatgtacgg
     2401 acatggcaca acgtcgccat cggcacggtc tccacgcgtg attacaacta ccggactgcc
     2461 tccatgccga tggatgcgac ggtcagcgtg cgtaatgatg cggtcaccac cggggagcat
     2521 taccgcgatg caccggctta tcgtgaagcg ggggatgaca ccagcccgga gccggaaacc
     2581 gaatctggtg cgttttacgc ccggcttcac cacgagcggg aactcaacag gtcggcccgc
     2641 attcacctgt tcagcaacgc cgcacacctc tcccccgggc aggtgctgga gccgcagggg
     2701 gatgtcattg cggacctgaa ggaagggatt gttctcacac tggtgactta ccggggggcc
     2761 cgggattccc ggctgcatgt gtcagcatgg ggaatgcctt acaccgaacg ttactgtttc
     2821 cgtccggcag aaatcccgcg tccggtcatc ccgggtacgc ttccggcacg gattgagagt
     2881 cgcgggaaga acgatatcta tgcccatctg gacgggcagg ggcgttaccg ggtgaagatg
     2941 gactttgacc gggaggagac ggaacccgga ttcggctacc tgtggctgcg gatggcaaaa
     3001 ccctgtgcag gtgaaacgct gggctggcat acgccgctca ttgatggcac cgaagtggcg
     3061 attgcataca gcaatggcga tattgacctg ccctacatcg cgtatgcact gcacgacgct
     3121 gagcatcctg acctcgtgag ccgggacaat cacacccgga acatcctcag aacgcctgcg
     3181 aacaacaagc tgcggatgga agacagacgc aatgaggagc acatcaaact cgctacggaa
     3241 tacggcaaga cacagcttaa cagcggacat ctggtggaca gccaggggca actgcggggt
     3301 aaaggcacag agctgcgtac cgatgagtgg ggaaccctga gagcagggaa gggacttttt
     3361 gttgctgcgg atgcgcaggc gaaagcacag ggcgaggcac tggacatgga tgccgcgctg
     3421 aaggaaatcg acaggcttaa tcagcaactg cagcagctgg aaaatgccgc agaacgggca
     3481 caggcgctga aggctgatat tgacagtcag atacagatgt ttgagcagcg gctgaaaccg
     3541 ctcaatgaag ccgtgctttt ctcggcgccg gaaggcattg ccctgaccag cggtgaagat
     3601 gtgcagctgg ctgctggtca gaacctggct gtcaacgccg gaggtgatat cagtaccgga
     3661 acaatgggca acctgaccgc gctggccgga gagaggctgg ggctttttgc caggagcgga
     3721 caactgagcg tcaaatccgg ggaggggcca gtagaggtac aggcgcaaaa cggcagcatg
     3781 cggttgtttg ctgagaagaa gctgacgctg agttcggcaa gtgatatctc ctttgccggg
     3841 aaaaaacgta tcacgctgat tggcgggggg agttacctgc ggctggaggc ggggaaaata
     3901 gagtacggta cgacggtgtc gtatctgcgc aaagttaaac ggactatagc ggcgggggct
     3961 gcgtcacagc agccggaaat gccttggctt gccgacccgg ttaaaccctc tgccagtgcg
     4021 ctgatgcagt atctgtatca cgacgatgtc ccggtagtgg gcgcgccttt caaagcgact
     4081 cttgctgatg gcactgtacg ggaaggtaac cttgacggtg cgggatatct gcatctggaa
     4141 gacgtcccgc cagggagagt gctggtggag ctcgggccgg ataccagaga ttatttacgt
     4201 aaaaataaga ccgataatcg gttgtttgtt gggacatctc tcagcgaggc tgacattgac
     4261 tcactgatag cgaagtacag cgaaggtgtg gaatgagtgg agtaaacgtt gcctggttta
     4321 aggaaaccag cctggaaacc ggacagtgga tctgggggac gcttcagggg gcatttaatg
     4381 aaaaacagac cattgggcag atcatcgttg atgcggtgat cggcatgatc ccgctggtcg
     4441 gcgatgtaac ggcagtgcga gatctgatag cggtttcgat aggactggca aaagaaccga
     4501 agaaacggga aagtgtgact gaatgggtgt ttctggtgat cctgctgttt gcgctaatcc
     4561 cggtaattgg tggcgtgata aaaggtgtcg gacgtctggc cctgcgtgtg acaacaaaag
     4621 tggcagagaa tgtggaaacc cttggcgctg tgattaagtt tctgaaccgt atcggcgaag
     4681 gtgatgcggt gaagtggctg aataagctga atgtcgttga gtatcaggca cagttgattg
     4741 aaaagatggg ggatttttgc aacacgatga ttaccgccat caataaaatc ctgaaagccc
     4801 gggtcgggcg gatgttgccg gagaaatggc aggcggattt actcttggtt cgggatggtt
     4861 ttgcggcact gcaacggctg gcgtcgagaa tgatcccgca ggcgttgaaa gagctggatg
     4921 cgaaactgcg ggtgttgcag gcgctggttt ataaaggtga aatacataca atcacgacgg
     4981 ggatgggacg tgctgaagtg cgccgtgagg ctgaagcata tcttattgag cgtccgctgt
     5041 gtgaagcgcc gaagcagggg acgcggtatc cgagtgttca tgcggaggta attcacgaag
     5101 acagattgcg gcttaaatat tccagggagg gatatccaga tttgttggga tataaaggaa
     5161 aaagtccggc atttggtgat gagcaagtat ttacaaatat tgcctctttt agcggcgaga
     5221 ttaccgcaaa aagtgcaagt gagatgggcg gtagtacgat ttttcgcgct tttggtaatc
     5281 caacagaatt agcacccaac ggttcttggg cggctggcgg attctggggg tatcaaaaag
     5341 tgccgggcag tgctgaagaa tggcgtcagc tttcagctgt actggatgag tggaatggta
     5401 acggttttct ggtggtgctg catttgccag aagacctggc aactcgtatg ccgggtagca
     5461 atgcttggca cgggaaaatt gctgaacagt tcggacagaa gagacctgac cagtatctgc
     5521 aaggtggtgg cgaacaggta attattaatc tggatgaaac gttgaaaaca gagttgaacc
     5581 gcatcgggga gcaggtaaaa gccagcggtc aggctgtcag tattgaaatt aatggcgtaa
     5641 atgccgagtt ttatccgacg ggctggaaag aggtggaagg cgtgcatggt tacagtaagc
     5701 cggaagggat agaaccgccg tatgtgacgt atacccgtga actgcatgac gatgaactgc
     5761 gcagcaaagt gacagagcga acgatactta atgcggcccg cattgagtac cagaccacga
     5821 caggaagcga acaatgatta ccattgaaaa aacaaaatac ccgatgagcg atgagcaggt
     5881 acgtcgcaaa ttattctggg tgttacagcg gctgagcagc tatagccttt ggcagcgcaa
     5941 acgggatgcc tgggcctatt ttacccagca gtatgaacag gcgctgaaaa actggccgga
     6001 agagataacg aaggggtttg atcccgaggc gataatctgg gcatatgatg ctttgcgtct
     6061 ttacgatgaa gggctatcag aattggcagt gggaaatcgg caggtatggc agcgtttaac
     6121 gggagaattt catcaacttg ctcgcccagt ggatttaatt gaatcttatt tttatctccc
     6181 ctgccatgaa cgaggtgtac agcaagaacc atacccggta gaagtagaaa agcttaataa
     6241 gctgaagatt gcagcaaaaa tacatggtga ctatctaatg tatccaccac ataacaatgt
     6301 atgcaacttt ttcgatgctg catatttatt agaattagat aattataaat ataattttaa
     6361 taagttagcg tatccggttt tcccagaaaa tctcccccct gttccagaac gcagcgatat
     6421 tattattaaa accgatgacc cggttccctg tgacggcatc tgggaaccgg taaagattca
     6481 ttacgaccat aaactattga ttattaaaga tggtatcagc
//
LOCUS       DAZGBC010000219         6485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGBC010000219 DAZGBC010000000
VERSION     DAZGBC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.220"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(176..2209)
                     /gene="betT"
                     /locus_tag="ACHK1J_004593"
     CDS             complement(176..2209)
                     /gene="betT"
                     /locus_tag="ACHK1J_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO7913764.1"
                     /translation="MTDLSHSRENDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2338..2925
                     /gene="betI"
                     /locus_tag="ACHK1J_004594"
     CDS             2338..2925
                     /gene="betI"
                     /locus_tag="ACHK1J_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO7913765.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2939..4411
                     /gene="betB"
                     /locus_tag="ACHK1J_004595"
     CDS             2939..4411
                     /gene="betB"
                     /locus_tag="ACHK1J_004595"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO7913766.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPVE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4425..6113
                     /gene="betA"
                     /locus_tag="ACHK1J_004596"
     CDS             4425..6113
                     /gene="betA"
                     /locus_tag="ACHK1J_004596"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO7913767.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSTE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKDALPRSTAGYYVANGMPVRAKKMSRDLN"
BASE COUNT         1611 a         1777 c         1765 g         1332 t
ORIGIN      
        1 attcagctaa tacaaaataa aatcgttaat taataaaata taaaatcatg aagtgataca
       61 agaggaattt caggatatga catgacaatc atatataata tatgcataat tttttacagc
      121 gagtcaatga ttcccgcgcc atatgcagac atggcgcggt tttatgcaat aacaatcacg
      181 cgtccgggaa catcacgcta tggcccggcg cttcacgatg gagatgaata aagttaaggt
      241 gccgctcgta ctggtcaaga atatcggtga tcacctgctc tttgctgtag tccatcaggt
      301 cgttgccctg gctgccttct aacaggaagg tttccagccg gtagtaggtc gatttcccgc
      361 tacgtgcgcg gtaggtaaag cccggcaccg agtattgctg cggccaaatc tgatagacaa
      421 agttttgctc ttcgcccata tgcaccaaca aatccagatg ccccaactgt tgtccctctt
      481 ccggcggtag gctttttagc tccacatacg cgccgcgcaa ccgcagctct tgcgccactt
      541 cttccattgc cgggtaacag accgtctcca tcatctgttt agtgtaacgc gtgcccggat
      601 aattcatcag gcgcgagaga cgttttttcc agctcaggcg atcctgaagc cccagcggtc
      661 gcggtgcggt atcgcggttg gcactttcac ggcggtaatc ttctaccttc agagatttat
      721 acaaccccgc catcacgaag aagatcacaa agctgaacgg cagccccata atcaccgtgg
      781 tgttttgcag cgcggatatc ccgttggtca tcagcatgcc gagcgtcagc aggccaatcg
      841 ccaccgacca gaagacgcgc agccagccgg gggcgtcgct gttgatatct ttaagctgcg
      901 aggtgaaatt ccccagcacc agcgccccgg agtccgccga ggtcacataa aacagcaggc
      961 cagtaatggt ggcgacggag gcgctaaagg taaacgccgg atactgcgcc agcaggctgt
     1021 agaagccgcg ctccggatgg accatcgctt cctctgcaaa tgccgcgccg ccgtggatga
     1081 tttcatacag cgcgctattg ccgaacaccg agagccataa cagcgtgaag gtaaacggaa
     1141 taatcaacgt gcccagcacg aactggcgaa tggtacgccc acgcgagata cgcgccagga
     1201 acaagccgac aaacggcgac catgccaccc accatgccca gaagaagagc gtccagttat
     1261 tcatccactc aactggacgg tcgaaggcaa aactgttgag cgtcatgccc ataaagcgat
     1321 tcacatagtc gccaacattc agcaccagtg cattaagcag gaacgaagtg tcgcccataa
     1381 acaacacgaa caggatcaat cccagcgcca gcgcgacatt aagctccgat aacacgcgaa
     1441 tgcccttatc gacaccggat gtcaccgaga tagtggcgat tatcaccgac aaggcgatca
     1501 gtgccgcttt tgccgccatc gaatcgggaa tatcaaacag tacgctcaag ccatagttaa
     1561 gctgcaccac accgataccg agcgtagtgg caataccgaa gatagtgccg atcaccgctg
     1621 caatatccac agagtgacct atcggcccgt taatccgttt accgaagatc gggtacagcg
     1681 ccgagcggat ggtgagcggc aaattataac gatagctaaa gtatccgagc gccatgccca
     1741 tcagcgcata catcgaccag ccggttaagc cgtagtgaaa cagcgtccag accatcgcct
     1801 gacgcgcggc ctcaatcgtc tgtcccgcac cttccggcgg ctgcatatac tgcgttaccg
     1861 gttcggctac ggaaaagaac atcagatcga taccgatccc ggcagcaaac agcatcgccg
     1921 cccaactcag caggctgaat tccggtttgg attgttctgg cccgagcttc accgaaccaa
     1981 aacgcgaaca agcgatacag accacaaaga caatatagag cgttgccgcc agcagatagt
     2041 accaaccaaa ggttttagaa acccagtcca gcgtgcggcc aatccacagg gccgagaagt
     2101 cgcgaaacag gatcgttgtc agggaaaaca acaaaatcag tccggcggag gtgtaaaaca
     2161 ccaccggatt gattttgtca ttttccctgc tgtgtgaaag gtctgtcatc cagtatcccc
     2221 actgttattg ttaccattta aaatcaaatt cgtaacaatt aagacacatt ttatattgaa
     2281 cgtccaatca ataaccgctt taatagataa acaacgctga tgaatggagt ggcgaaaatg
     2341 cccaaattgg ggatgcagtc gatccggcgc agacaactga tcgacgccac actggaagca
     2401 ataaatgaag tgggcatgca cgatgcaacg atcgcgcaga tcgcccgccg tgcaggcgtt
     2461 tctacgggga tcatcagcca ctatttcagg gacaaaaatg gtctgctgga agcaaccatg
     2521 cgcgatatca ccagtcagct acgtgacgcg gttttgaatc gattacatgc acttccgcag
     2581 ggcagtgcag agcagcgatt acaggcgatt gttggcggaa acttcgatga aacgcaggtg
     2641 agcagtgcgg cgatgaaagc ctggctggcg ttctgggcca gcagtatgca tcagccgatg
     2701 ctctatcgtt tacagcaggt cagcagccgc cgcttgctgt cgaatctggt gagcgagttt
     2761 cgccgcgaat tgccgcgcca ccaggcacag gaagcgggct acggccttgc cgcgctgatt
     2821 gacggattat ggctgcgcgc ggcactgagc ggcaaaccgc tggataaacc tctcgcacac
     2881 tcgctgaccc gtcactttat tactcagcat ttacccaccg attaaccgag gagacgtgat
     2941 gtcccgaatg gcagaacagc agctttatat acatggtggt tatacctccg ccaccagcgg
     3001 tcgcactttc gagaccatta acccggccaa cggtaacgtg ctggcgaccg tgcaggccgc
     3061 cgggcgtgag gatgtcgatc gcgccgtgaa gagtgcccaa caggggcaaa aaatctgggc
     3121 ggcgatgacc gctatggaac gctcgcgtat tctgcgtcgg gccgttgata ttctgcgtga
     3181 acgcaatgac gaactcgcaa aactggaaac cctcgacacc ggaaaagcat actcggaaac
     3241 ctcaaccgtc gatatcgtta ccggtgcgga cgtgctggag tactacgccg ggctgatccc
     3301 ggcgctggaa ggcagccaga tcccgttgcg tgagacgtca tttgtttata cccgccgcga
     3361 accgctgggc gtggtggcgg ggattggcgc atggaactac ccgatccaga ttgccctgtg
     3421 gaaatccgcc ccggcgctgg cggcaggcaa cgcaatgatt ttcaaaccga gcgaagtcac
     3481 cccgcttacc gcgttaaagc tggctgaaat ttacagcgaa gcgggcctgc cggacggcgt
     3541 atttaacgtg ttgccgggcg tgggcgcgga gaccgggcaa tatctgaccg agcatccggg
     3601 cattgccaaa gtgtcattta ccggcggtgt cgccagcggc aaaaaagtga tggctaactc
     3661 ggcggcctct tccctgaaag aagtgaccat ggaactgggc ggtaaatcac cgctgatcgt
     3721 tttcgatgat gcggatctcg atctcgccgc cgatatcgcc atgatggcaa acttcttcag
     3781 ctccggtcag gtgtgtacca atggcacccg cgtcttcgtt ccggcgaaat gcaaagccgc
     3841 atttgaacaa aagattctgg cgcgcgttga gcgcattcgc gcgggcgacg ttttcgatcc
     3901 gcaaactaac ttcggcccgc tggtcagctt cccgcatcgc gataacgtgc tgcgctatat
     3961 cgccaaaggc aaagaggaag gcgcgcgcgt actgtgcggc ggcgatgtac tgaaaggcga
     4021 tggcctcgat aacggcgcat gggtcgcgcc gactgtgttc accgattgca gcgacgatat
     4081 gaccatcgtg cgtgaagaga tcttcgggcc agtgatgtcc attctgacct acgagtcgga
     4141 agacgaagtc attcgccgcg ctaacgatac cgactacggc ctggcggcgg gaatcgtgac
     4201 ggcggacctg aaccgcgcgc atcgcgtcat tcatcagctg gaagcgggta tttgctggat
     4261 caacacctgg ggcgaatccc cggtagagat gcccgttggc ggctacaaac actccggcat
     4321 tggtcgcgag aacggcgtga tgacgctcca gagttacacc caggtgaagt ccatccaggt
     4381 tgagatggct aaattccagt ccatattcta accaggaggt ttatttgcaa tttgactaca
     4441 tcattattgg tgccggctca gccggcaacg ttctcgctac ccgtctgact gaagatccga
     4501 atacctccgt gctgctgctt gaagcgggcg gcccggacta tcgctttgac ttccgcaccc
     4561 agatgcccgc tgccctggca ttcccgctac agggtaaacg ctacaactgg gcttacgaga
     4621 cggaacctga accgtttatg aataaccgtc gcatggagtg cggacgcggt aaaggcctgg
     4681 gtggatcgtc gctgatcaac ggcatgtgct acatccgtgg caacgcgatg gatctcgaca
     4741 actgggcaaa agaacccggt ctggagaact ggagttatct cgattgcctg ccctactacc
     4801 gcaaggccga gacccgcgac gtgggcgaga acgactacca cggcggcgac ggcccggtga
     4861 gcgtcaccac ctccaaaccc ggcgtcaatc cgctgtttga agcgatgatt gaagcgggcg
     4921 tgcaggcggg ctacccgcgc acggacgatc tcaacggcta tcagcaggaa ggttttggcc
     4981 cgatggatcg caccgtgacg ccgcagggcc gtcgcgccag caccgcgcgc ggctatctcg
     5041 atcaggccaa atcgcgcccg aacctgacca ttcgtactca cgctatgacc gatcacatca
     5101 tttttgacgg caaacgcgcg gtaggcgtcg agtggctgga aggcgatagc accattccaa
     5161 cccgcgcgac agcgaataaa gaggtgctgt tatgtgcagg tgcgatcgcc tctccgcaga
     5221 tcctgcaacg ttccggcgtg ggtagcactg aactgctggc ggagtttgat attccgctgg
     5281 tgcatgattt acccggcgtc ggcgaaaatc ttcaggatca cctggagatg tatctgcaat
     5341 atgagtgcaa agaaccggtt tccctctacc ctgccctgca gtggtggaat cagccgaaaa
     5401 tcggtgcgga gtggctgttt ggcggcaccg gcgttggtgc cagcaaccac tttgaagcag
     5461 gcggatttat tcgcagccga gaggaatttg cgtggccgaa tattcagtac catttcctgc
     5521 cagtagcgat taactataac ggctcgaatg cagtgaaaga gcacggcttc cagtgccacg
     5581 tcggatcgat gcgctcgcca agccgtgggc atgtgcggat taaatcccgc gacccgcacc
     5641 agcatccggc gattctgttt aactacatgt cgcacgagca ggactggcag gagttccgcg
     5701 acgcaattcg catcacccgc gagatcatgc atcaaccggc gctggatcag tatcgtggcc
     5761 gcgaaatcag ccccggcacg gaatgtcaga cggatgagca gctcgatgag ttcgtgcgta
     5821 atcacgccga aaccgccttc cacccgtgcg gtacctgcaa aatgggttat gacgagatgg
     5881 cagtggttga cggcgaaggc cgcgtgcatg ggctggaagg cctgcgcgtg gtggatgcgt
     5941 caattatgcc gcagattatc accggcaatt tgaacgccac gacgattatg attggcgaga
     6001 aaatagcgga tatgattcgc gggaaggatg cattgccgag gagcacggcg ggatattatg
     6061 tggcaaatgg gatgccggtg agagcgaaaa aaatgagtcg tgatttgaac tgactcagga
     6121 accgatcaga ttgagagtta ccgttccagg tatggggaca gaatcgttat ataaaacata
     6181 ggggtaactc tcatgcttca tagtgacaac ctcgccagca aattctaagc tgacttgctc
     6241 aatctggctg atgaaggtgc tatgcataca cagataaact acagatgaaa taccactcag
     6301 cttaagaatt acgccgctaa tgttccttgc tatggttacc tgttcattcc cgctcacggt
     6361 tagcatgtac gaaaagggtt cttgtaacga gtacaaaaat aagtcagacc aaaaccacat
     6421 ggagctttaa acacggttcg tataatttat tagcagcgat ttatttttac tcttctttaa
     6481 ttaat
//
LOCUS       DAZGBC010000220         6464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGBC010000220 DAZGBC010000000
VERSION     DAZGBC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.221"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2757
                     /locus_tag="ACHK1J_004597"
     CDS             <1..2757
                     /locus_tag="ACHK1J_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7913768.1"
                     /translation="MVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVILAQKAD
                     AAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFNRLAAAD
                     EGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDGGADISG
                     SFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESRTFSIGQ
                     LKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGTSAVGGA
                     SGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSSALTVSD
                     GGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSGEYDFMT
                     GAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFHTLTAGS
                     MDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLVTAPADT
                     GADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDAETPNPV
                     EAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVNSSAVRD
                     AGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNYTHVQ
                     VGADKKHELDGLDLFTGVTMTYTYSNAGSDTFSGKTKSVGAGLYASALFDSGAHIDLI
                     GKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELVYGAVSG
                     KQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGE
                     TVLRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDNAVNASF
                     RYSF"
     gene            complement(3324..5996)
                     /gene="rcsD"
                     /locus_tag="ACHK1J_004598"
     CDS             complement(3324..5996)
                     /gene="rcsD"
                     /locus_tag="ACHK1J_004598"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO7913769.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVNIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1578 a         1474 c         1795 g         1617 t
ORIGIN      
        1 atggtgaacg gacttgcggg agacgcactt cataaaattg gtaagggaac actggtggtg
       61 gccggcagtg gtgaaaatcc tggcaccctg aatacggggg acggaacggt cattctggca
      121 cagaaggctg atgctgcagg cagggtccgg gcattcagtg aagtcagtat tgtcagcgga
      181 cgaccagttg tggtgctgca ggacagtcat cagattgaag gggaccgcat tcgctgggga
      241 taccggggag gtacgcttga tatcaacgga aatgacatag ccttcaaccg cctggctgcg
      301 gctgatgagg gggcagtact cacaagccgg gcgcgtcctg ccacagtccg tctggatttc
      361 agccagtcag ggcagaaggc cgtgatgtgg catggtcatt ttaccggtaa cctcagcgtc
      421 ctgaacaaca cgtcatctgc ggtggatttt atcatggatg gcggagcgga tatatccggc
      481 agttttacac agcagggcgg gggactgtat atccaggggc atccggttgt tcatgctgtc
      541 agcagtgagg cggttgcgac tgcattgcga aagcagggtg ataattctgt tctgacgcag
      601 ccggtatcat ttgctcagaa ggactgggag agcagaacgt tcagcatcgg tcaactgaaa
      661 ctgaaggggg cggcattcag cctttcccgc aatgcgacac tgacgggaga tattgatgct
      721 gataatgcca ccatggtgct gggaagtgac tcgctatatc tggatatgaa ggacggcacg
      781 gggagcagca gtgcgccggt taaggggacg tctgccgtgg gcggtgcatc cggtagcagt
      841 acatttcgcg ggaatgtgaa tatgcgtcat tctgccctga cggtccgcga ccattttact
      901 ggcagtataa cggcatcgga cagccggata gctgtgagtt cagagaatgt gcgtctggaa
      961 gggaattccc gcctgacctc ttctgcactt actgtcagtg acggcgggcg tttacatgtg
     1021 aaggggggac tggagacaga tggtggtgtg acactggaca gaggaaccct tttggtggac
     1081 gggggaagtg tcaggaatga tgtatatgaa cgtctgctgg cctggtcaga ggagcgtggc
     1141 gggctgaacg gaagcggtga gtatgatttt atgacggggg cagctggtct cctgcggagt
     1201 tatgtcagag ggagtgcagg taatgtgaat ctgcagaatg cggcctggat gatgacggga
     1261 aactcatccg tgaagcatct tgagtcatcg ggaagtgctc tgtatttcag caggccgggc
     1321 ggggagttcc ataccctgac ggcaggcagt atggatatca gcgacagtgt tctggtgatg
     1381 cggacggacc ttaaccactc agaccagctt cgggtgacag aatcgctgcg gggtaagaat
     1441 aacctgttgt tggttgattt tactgagcgt tctgacgggc agaaggcgct gaatatccct
     1501 ctggtgacgg cgccggccga taccggtgcg gatgtgttca gtgtgaaaac gcgtgatacc
     1561 ggcttcagtc atatcacacc tgttgtcagg gctgaacagg ggacgggcgg cacggcctgg
     1621 cagctgaatg ttgtgcagcc ggaaactgcc gcagagcctg tggcgacgcg gcaggatgca
     1681 gagacgccga atcctgtgga agctgtatca ccgctccctt caccggtttc tgctccgtca
     1741 tcggctccgg aggcgtcagt gacggatgta ctgaccggag agaatgcggg ggaatcaggt
     1801 gagtaccgtg atgagacccg gtggctgctg acaggatacc ggagtacggt taatagctcc
     1861 gcggtcaggg atgcggggat gctgatgtca atggggcacc gtaacttcat taatgaggtc
     1921 aacaacctga acaaacgtat gggtgacctg cgggacatca acggcgaggc cggtgcatgg
     1981 gcccgtatca tgagcggaac cggttctgcc ggcggtggct tcagtgacaa ctacacgcac
     2041 gttcaggtgg gtgcggataa aaaacatgaa ctggacggtc tggacctctt caccggggtg
     2101 accatgacat atacctacag caacgccggc agcgacacct tcagtggtaa aacgaagtca
     2161 gtgggtgcag gtctgtacgc ttcagccctg tttgattccg gggcccatat cgacctgatt
     2221 ggtaagtatg ttcaccatga taatgagtat acggcgtcct ttgccggact cggcacaaaa
     2281 gactacagct ctcattcctg gtatgccggt gctgaagtgg gttaccgcta tcatgtcaca
     2341 gaggatgcct ggattgagcc acaggcggaa ctggtctatg gtgctgtatc cggtaaacag
     2401 ttcagctgga aggaccaggg gatgagcctg tccatgaagg acaaggacta caatccgctg
     2461 attggccgta ccggtgtgga tgtgggtaaa tcgttctccg gtaaggactg gaaagtgacg
     2521 gcacgtgctg gtcttggcta ccagtttgac ctgctggcga acggagaaac ggtactgcgt
     2581 gatgcatccg gagagaagcg tattaagggc gggaaagacg gccgtatgct gatgtctgtg
     2641 ggtctgaacg cagaggtccg tgacaacatc cgttttggtc tggagtttga gaaatcagcc
     2701 tttggtaagt acaacgttga taatgctgtc aacgccagct tccgttattc attctgacgg
     2761 ttgttactga aggggattat ggcctgacgg ctccctgtaa ataaagacag gcacagaaga
     2821 ttgtgagtta cttagtgttc caccaacaag taaggtcgta actctgtgcc tgcttcgtaa
     2881 gcgtacagtg agggccatcg tcataactga agcatccgac gacgtggtgt ctaccagagt
     2941 gtcagatatg caggaaaata tgagctccga cagaaatgca gtaaaaaaag agagtctcgt
     3001 acacccctcc agaaaactcc agggctgtaa gttgtattaa aaagctgatt tttctttagc
     3061 gatacagacc tcaataagta taacaccctt cagttactgt ggttatgcac aggtgttatt
     3121 tttaactgtg tggataaaca gtgtcgatta ttcagattgg ttatggggca tgtatgggac
     3181 atttttaatc ggcgaaatac gtcgaagttc gtcgatatgg taaacgaatc atctatccaa
     3241 cccttgaaaa acggcgctcc tggacgatct tcgtcgattt ttaaaatgca acaagtacgt
     3301 tgttcatgta ataggctacc ttgctacagc aagctcttga cataactgtc aatgtcgctg
     3361 atgtattttt ctattcctgg aacatccttc tcacgaatca gatgttccag cgtttcacat
     3421 aactgcttgc cgggtaccag atttagcatg gcaaatacgc ctttaagacg atgagccgtt
     3481 tgggctaacg cagcaaagtc actggttgct gcttcagtat acagcctctt aacatcatcc
     3541 ggtactgtgt ctacaaagag cgcataatag ccgctggcat ggagttgcgc attttcatct
     3601 ccgcccagag gcgattctgt cacctcttcc tgcgccagtt gcacttcaat taattgtaag
     3661 accgcttcct gcatagcgtt gctcatattg aagttgacgc acaattgacc aggcccaatt
     3721 tcccgtacgc cagactcatc atcgcttaaa agcaagccag aggcagtaag attagacgga
     3781 ttatccgtta aaaagatatc ataatcttga ctaattaatc tttcatcggg tgtgatacag
     3841 gttgcacccc aattttctaa ctggcgagtg acaatattcc gaatttctgc cgaagtaaca
     3901 tccaccatta cgcagacatc atccagtaaa cgctcttctt cctcttcaac ttccgggtca
     3961 gctgcgagca ttttgatatg cacagagtag cgtgtaccaa gcccatcccg cgttttgatg
     4021 tttaaatgac cgcccagttt acgtgccagt tgatcgctca gccagaatgc cagcgggtcc
     4081 gccttgccat agcgatcgtt ttgggtctgg ttgataaacg ggaagtgcaa attatccatt
     4141 tcatgaatgt ttacgccttc tccggtgtcc aggatgcgga acgtcaggcg gtcttcggag
     4201 gactcatcct gatcaacctc aagggtgatt tttcccaatt gcgttgaggt cacggcatat
     4261 tgcatcagta gcagcaaaat acgtcgtaag gcatcgcgat cgccgcggcg catatcgtgt
     4321 gctttcagat gattgttaat cagcagttgc agacctttac gcttgatggc aggcaacact
     4381 gaaggcacaa cttcatcaat taaatcctgc acggagaaca gcaccgtctc acttttccag
     4441 ctatcgtccg caagcatgtt cgctaactgt atttcatcga ccagccgcac cagcacatct
     4501 gcctgattcg ccagttgttt gctttccggg gcgttgagtt tagccgcgct ctccgccagg
     4561 gactgtgcag gttctttcag cgcatcgcca atgtttttca taaaggtcat ccgcccctgc
     4621 tggtttttct catacagacg ctgcgcctgc ttgagtttct tgtttaccag cacttcgcga
     4681 tcctgatcgc gaataatgaa aatttgtgtg cgcggcgcga cctggctgcg gaacatgcgg
     4741 atctcataca gctcgttatt gatcgtcgcc tgaataatcc cctgatgctg ttccgccatg
     4801 gtggtgatgt tttgcagatt caaatgcggc agcaaatgat cggcaatttt gttacttatg
     4861 acagtgcggt tcgattcctg atcgtgaacc agcaggccga gcggcagcag tgagactatc
     4921 tcttcattga ttgcccgtaa aatgcgcaat tcgttattga ccgccgtgtt gggtagactt
     4981 tctgtactgc gactggagaa atggcggaat gtggtatagc caaataacgc cagcgccagc
     5041 aaaccgatgt tcagcagcag tggcagcaga atgttttgca acgtatccag caataaggtg
     5101 ccataaggaa cctgccagac caggcgcata tcggtagagt tgagtgccga ggagatttca
     5161 atcttcgtac tgttaaagtg gatactgacg ctatccgtcc cttctttctc attatcattg
     5221 tctcccgttg ccgtcgcgtc tggctcaagg cggaaactgt ccagcggcat acccggtggg
     5281 atcaaatcat taatcggcag atcaaaagcc acgaccgttg ccagatgtcc cggctggtta
     5341 aaggtggtac gcaaggtaaa gtaatgaccg ttctgccagg ccaggcggcg catgttagaa
     5401 aagctttcgc gttcatcgag agcgttggcc tgttgcaaca tctctgcacg acgtgaatca
     5461 acaatgtcac tgacggtcga ttctttaaat ccggaggtga gatctttgag gggcagggtt
     5521 gagatcagca ccagactgtt atcctgacca ttcaggtaat acattgacca cggtacattt
     5581 tctgcgcccc acaatgtgtc cagataggtg gacatccgtt gagtcatctc aagcgttgag
     5641 ttgtcgtgag agccaaagat taacgcttcc gttttgcggc gcggtttttc cagatagtag
     5701 acatcctgtt tcaggcgcgt ctcttgtaaa ccttcgccgg aggaggggga ggtcgtcgcg
     5761 gcaatgttgt cgtagatctg ccaggtcacg taacgccagg tatcgacgcg cttttggata
     5821 gcgtgggtaa tgtcgacaat ctggtaactt ttatctttca gccaggcgtt aacggcgctt
     5881 tgtaccatta cacccatcgt caccagtaac acaatgatca acagtaaaaa gaagcgggta
     5941 atgctccccg gtaggagtga aaagcgggtc gtggccgttg tctctttctg acgcattcgt
     6001 gtttatgacc tgttaaaact tcgcgaagtg atagggcagt ataaagggta cagtgtgaaa
     6061 ttccagactc ttacctgatt gatgattctc tattctcccg tgatatttat catcctgcat
     6121 tgggggaaat agatgtacaa gcgccatttt gctgtacaaa taacctacaa aaagcccaac
     6181 gaaaataatt gcaaataaac aaagattgct ggaaattatg ctgatgttaa ttatttgtga
     6241 aatggttaac aagcgttata gtttttctct ggtagcacag aataatgaaa agtgtgtaaa
     6301 gaagggtaaa aaaaaccgga tgcgaggcat ccggttgaaa taggggtaaa cagacattca
     6361 gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagttatt ctagttgcga
     6421 gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt aaga
//
LOCUS       DAZGBC010000221         6411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGBC010000221 DAZGBC010000000
VERSION     DAZGBC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.222"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..604
                     /gene="yfcV"
                     /locus_tag="ACHK1J_004599"
     CDS             41..604
                     /gene="yfcV"
                     /locus_tag="ACHK1J_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913770.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            686..3325
                     /locus_tag="ACHK1J_004600"
     CDS             686..3325
                     /locus_tag="ACHK1J_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO7913771.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAEDEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDVTHSHTRLDKETAYGK
                     GSLDGNSFRVSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNIMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQSDKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPMIQEQTQQVMPTEAPVSVSAN"
     gene            3348..4100
                     /locus_tag="ACHK1J_004601"
     CDS             3348..4100
                     /locus_tag="ACHK1J_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7913772.1"
                     /translation="MTNLFCSAKKSTMLLALLLTTASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDEQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTRSGQEFTVNNPTPYYVVISNASTQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LSYVNDYGARMPLYFKCGGDTCQVDVEQSRKG"
     gene            4116..4619
                     /locus_tag="ACHK1J_004602"
     CDS             4116..4619
                     /locus_tag="ACHK1J_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913773.1"
                     /translation="MRQIFVVGVGILLLGVSGIYNIILANSKPVDLILQVHVDPPPPC
                     SVTGSSVDFQTVNINDINGSNYRKDAGYTLSCPDRKADDLRMQLRGNTTTINGEKVVD
                     VGVPGFGIRIENADNNSLFSIGENNWTPFNITSQPKLNAVPVKQSGAQLSGADFSGSL
                     TMVVDYQ"
     gene            4616..5092
                     /locus_tag="ACHK1J_004603"
     CDS             4616..5092
                     /locus_tag="ACHK1J_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913774.1"
                     /translation="MKILLYLLLCMSSGIVNASPDITFKGTLVLPPACTISDGNTIEV
                     EFRDVIIDSIDGNNGREVVPYDIKCDAVTPGSSWDMTLTWIGTQTSYDDAAIETDVTG
                     LGIKLRQNGQPFRVNTPIQINADTKPQLEAVPVKAVDAVLTDGTFSASATLRVEYQ"
     gene            5095..5613
                     /locus_tag="ACHK1J_004604"
     CDS             5095..5613
                     /locus_tag="ACHK1J_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7913775.1"
                     /translation="MKTWFALIVMLLLAGNSTARADNNLHFYGNLLSKSCTLVVQGQV
                     LAEVHFPSISSRDLMSREQSDRVPVVFQLKDCKGPVGYKVRVTLTGTEDSDLPGFLAL
                     DASSGAQGVSIGMETVDGIQVPINNSAGAIFNLSNGANNLNFKAWLQAKSGREVTSGE
                     FTASLTAIFEYI"
     gene            5614..>6411
                     /locus_tag="ACHK1J_004605"
     CDS             5614..>6411
                     /locus_tag="ACHK1J_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO7913776.1"
                     /translation="MKIKVILFGMLLFGLNSSVQADVTFNNVQTYGASYGSGLTTSIV
                     YKIDVLTPEGAYYGTYSQGGIMVQAGVVPNLTWTGPGVPPDITIPPGRAPIVDDSYCP
                     GMTSGWVCHRLPVNVHVTGTTHGCPWVVTTSIYSTARPPQAGPYYYQGPNAHQSNCPT
                     EPVASYDISWDENTVVHNKSLSLQSTGGVIEKSLPTFLMKDGRLCDGSDFSDDRGAYC
                     RLVSQLMTFSVQGCDNNKVNVTANRHPVTDKQLHDMQVRVDTSERQSF"
BASE COUNT         1727 a         1489 c         1653 g         1542 t
ORIGIN      
        1 atgtttatat ttttaattat ttatggttta aggacaaata atgagtaagt ttgtaaaaac
       61 agctattgct gcaacaatgg taatgggtgc gttcgcttct acttcaacaa tcgccgctgg
      121 caacaatggt acagcacgtt tctacggcac cattgaagat tccccgtgct ccatcgttcc
      181 tgatgatcac aaactggaag ttgatatggg tgacattggt tcagggatcc tgaaaaacaa
      241 cgggacttct acaccgaaag ctttccagat ccatctgcaa gactgtgtgt ttgacaccca
      301 gacaacgatg accactacct tcaccggtaa cgcgtcttct accaacagcg gcaattacta
      361 caccatttac aataccgata ctggtgcggc atttaacaat gtcagcctgg ccattggtga
      421 cgctcaggga acctcttata aaagcggcgc gggtatcgaa cagaaaatcg taaacgatac
      481 ggcgaccaac aaaggcaaag cgaagcagac gctggacttt aaagcctggc tggtgggcgc
      541 tgctgatgcg ccagatttag gtaattttga agccaacacg accttccaga ttacttatct
      601 ctaagtgata cccccatagc tttcgggcta tggggttttt aaatggaatt cgcgcaaatt
      661 ataataatta cggatggata cgtgtatgcc tgaccattct cttttccgac tgcgggtact
      721 tccctgctgc gttgcattgg caatgtccgg gagttatgtc aatgcctggg ctgaagatga
      781 aattcagttt gattcccgtt ttctggagtt aaaaggcgac acaaaaatcg atctgaagcg
      841 attttccagc cagggttatg tcgaacctgg gaaatacaat ttacaggttc aactaaataa
      901 acagccgctg acggaagaat acgatattta ctggtacgcc tctgagaacg atgccagtaa
      961 aacctatgcc tgcctgacgc ctgaactggt cgcgcagttt ggcttaaaag aggatgtggc
     1021 aaaaaacctg caatggatcc acgacggcaa atgcctgaaa cccggtcaac tggaaggcat
     1081 tgatattaaa gctgacctga gtcagtcagc gttagtcatt tcattacccc aggcttacct
     1141 tgaatatacc gacatcaact gggatccgcc ttcacgctgg gatgacggta tatctggttt
     1201 aattgctgac tacagtatta ccgcccagac acgacatgaa gaaaatggcg gggatgacag
     1261 taatgaaatt agcggtaacg ggacggttgg ggtgaacctt ggcgcatggc gtcttcgtgc
     1321 cgactggcag actgattatt tgcatagtaa aagcaatgat gacgatgtta tcaacggtga
     1381 tgacacgcaa aaaaactggg agtggagccg ctactacgcc tggcgggcct taccgtcgct
     1441 aaaagccaaa cttggccttg gtgaagacta cctgaattct gatattttcg acggctttaa
     1501 ctacgtgggt ggcagtatca gcaccgacga tcaaatgttg ccgccgaatc tgcgcggcta
     1561 tgcgccggat atctccggcg tggcgcacac caccgcgaaa gtgaccgtca gccagatggg
     1621 ccgcgtgatt tacgaaactc aggttccggc ggggccgttc cgtatccagg atcttggcga
     1681 ttccgtctcc ggtacgctgc atatccgcat tgaagagcag aacggtcagg tgcaggaata
     1741 tgacattaac actgcgtcaa tgccgttcct gacccgcccc ggccaggtgc gctataaact
     1801 gatgatgggc cgcccgcagg agtgggggca ccacgtggaa ggcggtttct tctccggcgg
     1861 cgaagcttcc tgggggattg ccaacggctg gtcgctatac ggcggggcgc tggcagatga
     1921 acactatcag tcggcggcgc ttggcgtcgg tcgcgatctg tctgtgtttg gtgcggtggc
     1981 ctttgatgtc acccactcgc atacccgcct ggataaagaa actgcctacg gtaaaggttc
     2041 actggacggt aactcgtttc gcgtgagcta ttccaaagac ttcgatgaac tgaacagccg
     2101 cgtcactttt gccggatacc gcttctcgga agagaacttc atgaccatga gcgagtatct
     2161 cgatgccagc gacagcgaaa tggtgcgcac cggcaacgac aaagagatgt acaccgccac
     2221 ctacaaccag aacttcaggg atgccggtgt atctgtttat ctcaactaca cccgccatac
     2281 ctactgggat cgcgacgaac agactaacta caacatcatg ctctcgcact acttcaacct
     2341 gggcagtatc cgcaacatga gcatttccat gaccggatac cgctacgagt atgacaacca
     2401 gtccgataaa ggtgtttaca tatcgctcag catgccgtgg ggtgacagca gcaccatcag
     2461 ctataacggc aactacggca gcggttcgga cagcagccag gtggggtatt tcagccgtgt
     2521 cgatgacgca acccattacc agttgaacgt aggcaccagc gacaatcact ccagcgttga
     2581 cggttattac agccacgacg gatcgctggc gcaggtcgat ctcagcgcca actatcatga
     2641 aggacagtac acctcggcag gtatttcctt acagggcggc gcgacgctga ccgcgcaggg
     2701 tggcgcgctc caccgtaccc agaatatggg cggtacgcgc ctgctgattg atgccgatgg
     2761 cgtggccggt gttccggtgg aaggaaacgg tgcggcggtt tacaccaata tgttcggtaa
     2821 ggccgtggtg gccgacgtca acaactacta ccgcaaccag gcgtatatcg accttaacaa
     2881 cctgccggaa aacgccgaag ccacccagtc cgtggtgcag gcgacgctta ccgaaggagc
     2941 cattggctac cgtaagtttt cggtgatcag cgggcaaaaa gcgatggcgg tgctgcgtct
     3001 gcaagatggc agttatccgc cgtttggcgc ggaagtgaaa aacgacagcg cgcagaacgt
     3061 cggtctggtt gacgatgacg gcaacgtcta cctcgcgggc gtcaaacctg gcgagcatat
     3121 gatcgtttca tggggcggtg tggcccactg cgatattcat ctgcctgacc cgctgccagc
     3181 cgatctgttc aatggcctgt tattaccatg ccagcaaaca ggggcgatat ctccttcgat
     3241 gcctcatgaa attaagccga tgatccagga gcagacccag caggtgatgc caacggaagc
     3301 accggtatcg gtatccgcca attaataacg tgattaagga atgatccatg acgaatctat
     3361 tttgctctgc caaaaaatcc acaatgttgc tggcattgct actaaccact gccagcctgt
     3421 cagcacaggc ctctgtcacg ccagaccgta cacgcctgat ttttaatgag agtgataaat
     3481 caatcagcgt cacgctgcgt aataacgacc cgaaaatgcc gtatctggca caaagctgga
     3541 tggaagatga acagggtaac aggattagct cgccactgac ggtcctgccg ccggtgcagc
     3601 gcatcgattc catgatgaac ggccaggtga aggtgcaggg gatgccggat atcaacaagc
     3661 tgcctgccga ccgcgagagc gtgttctact tcaacgtgcg tgaaattccg ccgaaatcga
     3721 ataaacccaa cacgttacag atagcgctgc aaacgcgcat caagctgttc tggcgaccaa
     3781 aggcgctgga aaacgtcagc atgaaaaacc cgtggcagca taaggtgacc ttaacccgca
     3841 gcggtcagga gtttaccgtc aataatccta cgccgtacta cgtggttatc agtaacgcca
     3901 gcacgcagaa aaatggcaat ccggcagcgg gattttcccc catggtgata tcgccaaaaa
     3961 cctctgtgcc gttaaaagtc aaaatggata gcgtcccggt gctgtcttac gtcaacgact
     4021 atggcgcacg gatgccgctg tattttaaat gtggcggtga tacctgccag gtggatgtag
     4081 agcagagccg caaaggctga tgccgggaga gcgacatgag acaaattttt gttgtggggg
     4141 tgggtatatt actgctgggc gttagtggga tatataacat tatccttgca aattcaaaac
     4201 ctgttgatct cattttacag gtacatgtag atcctcctcc gccatgtagc gtaacgggga
     4261 gttcagtaga ttttcagact gtgaatatca acgatatcaa tggtagtaat tatcgtaaag
     4321 atgcaggata tacgttgtca tgccctgacc gaaaagctga tgatttacgt atgcagttga
     4381 gaggaaacac caccacaatt aatggagaaa aagtcgttga tgtaggggtt ccaggatttg
     4441 gtattcgaat cgagaatgca gataacaact cactattctc cattggagaa aacaactgga
     4501 cccctttcaa tatcacatca cagccgaaac ttaatgcagt cccggtaaaa caaagtggtg
     4561 cacaactttc aggggccgat tttagtggga gtttaacaat ggtggtggat tatcaatgaa
     4621 aatactcttg tatttattgc tctgtatgag cagcggtatt gtgaatgcat caccggatat
     4681 caccttcaaa ggaactcttg tattgcctcc ggcatgtact attagtgatg gaaacactat
     4741 cgaggtggag ttcagggatg taattatcga tagcattgat ggaaataatg ggcgagaggt
     4801 cgtgccctac gatattaaat gtgatgctgt tacacccggt agttcctggg atatgacatt
     4861 gacatggata ggcacacaaa caagttatga tgatgctgca atcgaaactg atgtaacagg
     4921 attagggatt aaacttcggc aaaatggtca gccatttaga gtaaatacac cgatacaaat
     4981 taacgccgat acgaaaccac aattagaggc tgtccccgtg aaggcggtag atgctgtatt
     5041 aacagacgga acgttttcag cttcggccac attacgggtg gaatatcaat gaagatgaaa
     5101 acctggttcg cactcatagt aatgctgtta ctggcaggaa atagcacagc gcgtgcagac
     5161 aataatttac acttctacgg aaatttatta agtaaatctt gtaccctggt agtacaagga
     5221 caagtgctgg ctgaagtcca tttcccaagt atcagcagca gagatttaat gtcacgggaa
     5281 caatctgatc gtgtgccggt cgtttttcaa ttgaaagatt gcaaagggcc tgttggttac
     5341 aaagttagag ttacgttaac cggtaccgaa gatagcgatt tgccagggtt tctggctttg
     5401 gatgcatcat cgggtgcgca aggtgtgagc attggcatgg aaacagttga tgggatacag
     5461 gtccccatca acaattcggc aggggcaata tttaacctga gtaacggtgc taataatctt
     5521 aactttaagg cttggttgca ggcgaaatct gggcgtgaag tcacttctgg tgaatttact
     5581 gccagcctga cggcaatttt tgagtatatc taaatgaaaa taaaagtgat tttatttggt
     5641 atgttattgt tcgggcttaa ttcttctgta caagcagacg ttacatttaa taatgttcag
     5701 acttatggcg catcttatgg cagtgggcta acaacatcta tcgtttataa aatagatgtg
     5761 ttaacccctg aaggggcata ttatgggaca tattcacaag gggggattat ggtacaggca
     5821 ggagttgtgc ctaacttaac ctggactgga ccgggtgtcc ccccagacat aacaattccg
     5881 ccagggcggg caccgatagt agatgattct tattgtcccg gtatgacctc tggttgggtt
     5941 tgtcatagac ttcctgtaaa tgtacatgtt acgggtacaa cccatggttg cccttgggtg
     6001 gtgacaacat caatttatag cacagcaagg cctccgcagg cggggccata ttattatcag
     6061 gggccgaatg cgcaccaatc aaattgtcca acagagccag tagcaagcta tgatatttcc
     6121 tgggatgaaa acaccgttgt tcataataaa tcattaagcc tgcaatctac ggggggggtt
     6181 attgagaaat cgttgccgac attcttaatg aaagatgggc gattatgtga tggcagtgac
     6241 ttcagtgatg accgtggagc ctattgtcgc ctggtatcac aattaatgac gtttagtgtc
     6301 cagggttgtg ataacaataa agtgaacgta acggcaaacc gacatcctgt aactgataaa
     6361 caactccatg acatgcaggt gcgtgttgat actagtgaac ggcaaagttt t
//
LOCUS       DAZGBC010000222         6331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGBC010000222 DAZGBC010000000
VERSION     DAZGBC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.223"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1040
                     /locus_tag="ACHK1J_004606"
     CDS             132..1040
                     /locus_tag="ACHK1J_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO7913777.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVLLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1260..3251)
                     /gene="tkt"
                     /locus_tag="ACHK1J_004607"
     CDS             complement(1260..3251)
                     /gene="tkt"
                     /locus_tag="ACHK1J_004607"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7913778.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3529..4287
                     /gene="loiP"
                     /locus_tag="ACHK1J_004608"
                     /gene_synonym="yggG"
     CDS             3529..4287
                     /gene="loiP"
                     /locus_tag="ACHK1J_004608"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO7913779.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4493..5413)
                     /gene="speB"
                     /locus_tag="ACHK1J_004609"
     CDS             complement(4493..5413)
                     /gene="speB"
                     /locus_tag="ACHK1J_004609"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO7913780.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5549..6280)
                     /locus_tag="ACHK1J_004610"
     CDS             complement(5549..6280)
                     /locus_tag="ACHK1J_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913781.1"
                     /translation="MKKWKVRSALIALVVLLVGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRA
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1538 a         1681 c         1651 g         1461 t
ORIGIN      
        1 ttcgaaaaca tacttttcta atgcaatgtt ttatcgctat acccctttat agaatctgaa
       61 ttctcacctg tcggctgttt tctcctcatc tcatgtgaca tattcccaca ctttactcaa
      121 ggaagattgt catgaaagca cctgtttcat taacctcatt tcgcccacaa aaatctcttg
      181 ctatcgccat cggcgtgctt gccgtggttt tgctgccatt tctctcgtat tacaccgtta
      241 atgaaggtga acgcggcatt ttgctgcgct acggaaaaat cgtaaaagtt gccgagccgg
      301 gtctgggttt taagatcccg tttatggaaa gcgtggagaa aatttccacg cgtaatcagg
      361 ctgtggtcta tcagggatta caggcctaca gccgcgacca gcaaccggca caaatgacag
      421 tatcagtcag cttccacatt aaaccttcag aagcaggcgc ggtttatacc acctacaaca
      481 ccattgaagc attaaaagat cgtctgattg tccgtcagtt gccaactcaa ctggagaacg
      541 ttttcggcca atataccgcc atcagcgctg tacaggatcg tacaaaactg gttcaggatc
      601 tgcaaaatgc catgcgcaaa gccgttgttg ggccagtggt aattgacggg gtccagattg
      661 aaaatattga tttttctgac gcttatgaaa agtcgattga agaccgaatg aaagcagaag
      721 tcgccattgc cacgcgtaag caaaatctgg aaacagagaa aatccaggcg caaatagcgg
      781 taactcaggc ccaggcagaa gcagacagta aactggcggc agccaaagca gaagcggaaa
      841 ctattcgcgt aagaggcgcg gcagaggctg aaactattcg tttaaaaagt gcggctgaag
      901 cagaagcaat ccgcctgcgc ggtgaagctc tgcgggataa tcccggactt gtagccctca
      961 ccactgccga acgttgggac ggtaaactgc ccgataccat gatccccggc agcacagttc
     1021 cgtttatctc tactaagtaa aaaaagggcc gctttcgcgg cccttgagag tgatggcaaa
     1081 agcaaaactg cctgatgcgc tacgcttatc aggcctacat acttcctgca atatattgaa
     1141 tttgcacgat cttgtaggtc ggataaggcg ttcacgccgc atccgacatg aacaacgcgc
     1201 actttaccaa taatctaaag ggccgctttc gcggcccttc tcacaaaacg tcttactgat
     1261 tacagcagcg cttttgcttt cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac
     1321 agcagctctg ccggagcaga ttcaccgaag gtggtcatac cgacgatagc accgttcagg
     1381 ccaacatact tgtaccagta gtcagcaata cccgcttcta ccgcaacgcg tgcagtaacc
     1441 gctttcggca gtacggattc acggtaagcc gcatcctgct tgtcgaatgc atcggtagac
     1501 ggcatggaaa ccacgcgcgc cttcacgcct tcggcagtca gtttttcgta ggcggcaaca
     1561 gccagttcaa cttccgaacc ggtcgcgatg aagatcagtt ccggctgacc ggcacagtct
     1621 ttcagcacat aaccaccgcg cgcgatgttt gccagctgct cttcagtacg ctcctgctgc
     1681 gccaggttct gacgggagag gataagcgcg gtcgggccgt cctgacgctc aacaccgtat
     1741 ttccatgcca ctgcagattc aacctggtca catggacgcc atgtagacat gttcggggtc
     1801 acgcgcagag aagcaacctg ctctaccggc tggtgagtcg ggccatcttc gcccagaccg
     1861 atggagtcgt gggtgtaaac catcacctga cgctgtttca tcagcgcagc catacgtacg
     1921 gcattacgtg cgtattctac gaacatcagg aaggtggagg tgtacggcag gaagccaccg
     1981 tgcagggaga taccgttagc aatcgcggtc ataccgaact cgcgaacacc gtagtggatg
     2041 tagttacccg cagcatcttc gttgattgct ttagaaccag accacagggt caggttagac
     2101 ggtgccaggt cagcggagcc gccgaggaat tccggcaaca gcgggccgaa cgcttcgata
     2161 gcattctgcg acgctttacg gctggcgatt ttcgccgggt tagcctgcag tttagcaatg
     2221 aactctttcg ctttggcgtc gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat
     2281 tcagcggctt cctgcggata agctttcgcg taagctgcga atttctcgtt ccatgcggat
     2341 tctttcgcct gacctgcttc tttcgcatcc cactgagcat agatttcaga cgggatttcg
     2401 aacggcgcgt atttccagcc cagctgttcg cgggtcaggg caatttcagc gtcgcccagc
     2461 ggcgcaccgt gggagtcgtg agtaccggct ttgttcgggg aaccgaaacc gatgatggtt
     2521 ttgcacatca gcagggacgg tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg
     2581 atggatgctg cgtcatgacc gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa
     2641 cgcattgcgg tgtcgtcggt gaaccagcct tcaacgtgac catcaataga gataccgttg
     2701 tcatcgtaga acgcaatcag tttacccagc ttcagcgtac ccgccagaga gcaaacttcg
     2761 tgggagatgc cttccatcat gcagccgtcg cccatgaagg cgtaggtgta gtgatcgaca
     2821 atgtcgtggc ccggacggtt aaactgcgcc gccagcgttt tttctgcaat cgccatacct
     2881 actgcgttgg caataccctg ccccagcgga ccggtggtgg tttccacacc agcggtgtaa
     2941 cccacttccg ggtggcccgg agttttagag tgcagctgac ggaagttttt cagttcttcc
     3001 atcggcagat cgtaaccggt gaggtgcagc aggctgtaga tcagcatgga gccgtggccg
     3061 ttggacagca cgaagcggtc acggtcagcc caggacggat tctgcgggtt gtgtttcagg
     3121 aaatcacgcc acaggacttc ggcaatgtca gccataccca taggggcacc cgggtgaccg
     3181 gatttggctt tctgtactgc gtccatgctc agcgcacgaa tagcattggc aagctcttta
     3241 cgtgaggaca ttttgactcc agatcggatg atgaagggca cgcccttaac gacttgacga
     3301 cagcgcgttt tgggctacgc cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat
     3361 gtacatggaa catccttttg ccgcttcaga aatctctgga tcatgctcgc atgttgcgca
     3421 atctactcgc ccgtccgctg cgcttttcct tatactgaga ctgagcgtcg gttcacctgc
     3481 aaacggcgca tttttagaat aatcctgacc ttgtgcggaa gaaaaaacat gaaaattcgc
     3541 gccttattgg tagcaatgag cgtggcaacg gtactgactg gttgccagaa tatggactcc
     3601 aacggactgc tctcatcagg agcggaagct tttcaggctt acagtttgag tgatgctcag
     3661 gtgaaaaccc tgagcgatca ggcatgtcag gagatggaca gcaaggcgac gattgcgcca
     3721 gccaatagcg aatacgctaa acgtctgaca accattgcca acgcgctagg caacaatatc
     3781 aacggtcagc cagtgaatta caaagtgtat atggcgaagg atgtgaacgc ctttgcgatg
     3841 gctaacggct gtatccgcgt ctatagcggg ctgatggata tgatgacgga taacgaagtc
     3901 gaagcggtga tcggtcacga aatggggcac gtggcgttag gccatgtgaa gaaagggatg
     3961 caggtggcac ttggtacaaa tgccgtgcga gtcgcggcgg cctctgcggg cggtatcgtc
     4021 ggcagtttat cgcaatcaca gcttggtgat ctgggcgaga aattagtcaa ttcgcaattc
     4081 tcccagcgcc aggaagcaga ggcggatgat tactcctacg atttactgcg ccaacgcggc
     4141 atcagtccgg caggtcttgc caccagcttt gaaaaactgg caaaactgga agaaggtcgc
     4201 caaagctcaa tgtttgacga ccatccagca tccgccgaac gcgcccagca tattcgcgat
     4261 cgtatgagcg cggacgggat taagtaaggc atggtggtgc caaacgtagt tcagataagg
     4321 catttacgcc gcatctgaca taaatggcac attcactacc tcgtgcgcat tgccggatgc
     4381 gatgctggcg catcttatcc ggcctacggg tgccaaacgt aggtcagata aggcgttcac
     4441 cccgcatctg acatgaatgg cacgttttac ccgtgcgcat cgcatctggt gcttactcgc
     4501 cctttttcgc cgcctgaata tacagcattt ccagcgccag cgttgccgct gccagagcag
     4561 tgatttccga ctgatcgtat gccggagcca cttccactac gtccatccca acgatgttga
     4621 gatctttcag gccgcgtacc agtttaatag cgcgatcgga ggtcaggccg ccaattaccg
     4681 gggtaccggt gcccggcgca aatgcaggat ccaggcagtc gatatcaaag gtcaggtaaa
     4741 ccggcatatc acccacaatt tgtttcacct gggcgataac gtcatccacg ctgcgatcgt
     4801 tcacctggca ggcgtccagc acggtaaagc cgttgtcttt atcaaattcg gtacgaatac
     4861 caatctgcac ggaatgattc gggtcgatca gaccttcttt cggcgcggta tagaacatgg
     4921 tgccgtggtc aaattcacaa ccgttcgcgt aggtatcggt gtgggcgtca aagtgtacca
     4981 gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag cggcagcgta acaaagtggt
     5041 caccaccgaa ggagagcata cgcttaccgg cagccagcag cttctcggcg tgtgcctgca
     5101 gtttttcgct catctcacgg gcatcgccga aggcatatac cagatcgccg cagtccacca
     5161 cgttcagacg ctcgcgcatg tcgaaattcc acgggaagcg gttgtgttcc caggccaggt
     5221 tagtcgaaac ctggcgaatc gccgccgggc cgtgacgacc accggcacgg ccagaggtgg
     5281 ccatgtcgaa cggcacgccg gtgatcaccc agtcggcatc gctgtcatac ggctggaagt
     5341 tcatcggcag gcgtaaaaaa ccaaaggcgt tggaaaccag tgagttatcg tattgatgac
     5401 ctaaggtgct catggatgga ctcctattac aaagtcgata taaaaaaacc cttccgcgtc
     5461 gttaggcccg acgaggaagg gttggatttg cgaaacacaa gtttgtgcta attatcgcct
     5521 taaatgggcg aggcatcaag cgaggttgct atttccgaca cggtggcggc gcatagccat
     5581 tgttatctgc tggaaaagct tgtccagagc acggtggcag cgcgttttgc gccgggcctt
     5641 gtaattcaaa ggcggcaaag ccgattaccc cggtattacg cacatcgccc tgcgccgaat
     5701 tggctgcgta ggaatcaaca acgttggcga actgaaaagc cgcttcggta tgtttatctt
     5761 tgcggaatcc tttaatcgct aacgaatctc cggcattcac aatatagccg ttgttattca
     5821 gcgaaccttg cttgccgttc agcacatcca ggccatcaac cgtagcaacg atctcgtagt
     5881 tggtgttacg actgtaatta cgaacatata attgataatt cattccgact ctggcgtgga
     5941 caatatagcg tggttcatta ttggatgaat cataagcccg cgtaataggt agagaattaa
     6001 aattaccatc gcgaatagca tattccagtt cgttaatacg aatgctataa acgcggtcgt
     6061 agccggaagg aacattagtc gaataactaa taacgatcat ctctgcagga tcgcgccagg
     6121 ctcgttcagc agatacgccc tgaaccgatg agtgcacatc cccaccccag gcggtgccga
     6181 cattgcccga tgcactgcta ttatactgcg cattactgga acaaccgacc agcagcacga
     6241 ctaatgctat caacgcgcta cgtactttcc attttttcat aaccgttccc tctgcattta
     6301 attaatgcag cgattgtagg gagattattt a
//
LOCUS       DAZGBC010000223         6275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGBC010000223 DAZGBC010000000
VERSION     DAZGBC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.224"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            128..821
                     /locus_tag="ACHK1J_004611"
                     /pseudo
     CDS             128..821
                     /locus_tag="ACHK1J_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1044..1340
                     /locus_tag="ACHK1J_004612"
     CDS             1044..1340
                     /locus_tag="ACHK1J_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7913782.1"
                     /translation="MPDQSGYCWTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1648..1764
                     /locus_tag="ACHK1J_004613"
     CDS             1648..1764
                     /locus_tag="ACHK1J_004613"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913783.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1983..3416
                     /locus_tag="ACHK1J_004614"
     CDS             1983..3416
                     /locus_tag="ACHK1J_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO7913784.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     FSSACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDENLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            4394..4957
                     /locus_tag="ACHK1J_004615"
     CDS             4394..4957
                     /locus_tag="ACHK1J_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913785.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHGCKHSMCMEIYRLLSETQTMLAGYYWVMEYTQNKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGDGYFHLCRAKDKYPVCIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            5097..5422
                     /locus_tag="ACHK1J_004616"
                     /pseudo
     CDS             5097..5422
                     /locus_tag="ACHK1J_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            5486..6048
                     /locus_tag="ACHK1J_004617"
                     /pseudo
     CDS             5486..6048
                     /locus_tag="ACHK1J_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
BASE COUNT         1831 a         1220 c         1387 g         1837 t
ORIGIN      
        1 ttcatttttg aatcaatatt ttctgaacag ggtaatagta cacgtattaa cagattatgt
       61 cagaaggccc cggtgatgcc ttctgacgat agattaatgt ggtattctat tggtatctgg
      121 agaccatatg gcaacagtta cagtacattc tccccgctgt aattcctata aagtgtacca
      181 ttatgatcac agcacctcag atcatgagcg tttccagtgc taatcctgca aacgtgtgta
      241 tttcaactgc attacatcta cgaaacccga aaaccaggca ttaaagagca aattgttgaa
      301 atggttcata acggagtcgg ggttcgcgat agctccagaa cgctgaaggt ggatatcaac
      361 accgtcattc tcactttata aaacgctcac cacgcaaagt aaccacatct ccagtcgcca
      421 gtgccgatgt gacgcttatc tgcgaacttg atgaacaatg gagttttgtc gaaaataaag
      481 ctcgtcagca gtggcactgg tatgcgtata acaccaaatc tggaggtgtg ctggcttaca
      541 cttttggtcc tcgcactgat gaaacatgtc gtgagttgcc ggaactcctg aagccattct
      601 ccacaggcat gataaccaga gataaccgga gcagttatac cagagaaatg ccacaggata
      661 agcatcttgt cggcaagata tttacccggc gcattgagcg caacaatctg acatttcgta
      721 ctcacatcaa acgaccagcc cggaaaacga tttgcttttc acgctccctg gaaattcacg
      781 aaaagccatt ggttcatttg tcgaaaacac tgtactactg actggagtca ttaccagggc
      841 ctcttttgct gttttttacc aatgaatgat aatgagaatc gacagcgata aggagaaagt
      901 aaaataacaa taatgctgtt gttttctatc gtattgattt ttaattattt attgtctttt
      961 tgctcagccc atttttttac gcagacaaat ttacccgggg aatacaaact gtaacgacag
     1021 ttcaccacac tatgggggat aaaatgccag atcaatccgg atactgctgg actccttcac
     1081 gcatcttacg gatgcctgaa ctgagccaat tgttaggtat atctcgttcg acaatttacg
     1141 aaaaaatgaa tccgttatcg aaatactacg atgcaacctt ccccaggccg gtacgtcttg
     1201 gtagtggttc tgtgggctgg cgttcatcag ctattgacga atggcttact ttacatactg
     1261 tacctgccag atcagcagtc aaaggtgtta atgacgaagt gcgacatacg ctttcttcag
     1321 atatgaccat gagcagataa gtttttgtca tcacgtgatc acctcaggag gaaaaagaag
     1381 cataagaaca atttagccag aagtttaatt gataaacaga atttccagca ttcattgttg
     1441 aaagagcagc tattgtcgct attgtagcgg taattgctgc tataaccgaa acgtattacg
     1501 aaaaaggaag catttatgtc ttacatcaaa ccggatatgc cgtattcagt atatgcgtga
     1561 ccatcaatga tagggaaggc aataattaaa gctcaataca ataaacaagt acctctgact
     1621 ctggttgcaa tgtcagtatt aacagcgatg tcaattgcct gtcagaatca ggttgacgta
     1681 tgcagccccg gaaatttacg tggggccgtt aatatttaca ctatggttct ggagaaaggg
     1741 aaaagaccgg gggaaaggta ttagtgaaat aatttcagga ggtattaaga atgagaaata
     1801 tgaatgatat ttcgtggtaa ataatacagt ctgcactgaa ttaaccttat cggtttatta
     1861 aacatcagat ctacaaatgc tctgtatttc tcagagcatc aggtctgaat agcagctatt
     1921 ttcgctattg tagcgggatt tgctgctatt ttactaaccc attactgaaa agagaggctt
     1981 ttatgtgctt acttgcacca gaaaatcctt atccgatata tgcactacca ccgctggtga
     2041 gaaatgcaat aattgaaact caaaaaaata cacaggcacc tttggctatg gtggcgacat
     2101 ccgcattaac agcgttctca agtgcctgtc agaatcagat tgacgtgtgc agacctggaa
     2161 atttacgtgg gcctgttaat ctttactctc tgattctggc tgattccggt gaaaggaaaa
     2221 cgaccgtgga taaagtgttt atgaaagcat tttatctcag ggatgaagcc ctggcggaag
     2281 aatacgcgaa actggttgag aattacagta cagaaaagga aatatgggag caaaagcaaa
     2341 aagcactgga gtcaaaattg cataaagaaa ttcgcgccgg taaagattgt aaggcaacag
     2401 agtcagagct taaaagacat ctgaataaat gtcctgttcc gccgcagata cgccggacaa
     2461 ttttcaatga gacaacgata gagggaatgt taaaatatta ttccgacagc aatcgctctt
     2521 ttgctcttgt atccagtgaa ggaggaataa tttttgatgg ccgggcaatg agtaaacttg
     2581 gaatacttaa ctctttgtgg gatggtggtt ctcttttcat cgacaggaaa tcatctcccg
     2641 gaattatttt gaaggatcca agactgacgg tatcggtgat gattcagcct gatgtttacc
     2701 agaaaggttt ttgtacgcga aaaaaagaac ttgtgaaaac gtcaggacat catgcaaggt
     2761 ttttgatgtg tcaaccaaca tcaacgcagg ggacaaggat aataaccgga gataattatt
     2821 catcacagta tcaggatttg tttgaacaga ggatcaatga gcttattgat gaaaatctgg
     2881 cgatgagtgg tgaacgacgt tgccttcact tttctcctca ggctgccaga atctggacgg
     2941 attattacaa cgatgtggaa tcgaagctgg ggggattggg gcctttaaga cattgccggg
     3001 aatatgctgc taaaaatgca gagtatatgg caagactggc aggacttatt caccattcga
     3061 gcggtgaaga gggggacatt tccccttaca ctgcagaaat ggcgagagaa ttagcaatat
     3121 ggtacggtaa tgagtatgtg cggttgtcta atccgttaac ttttgacaac cctgccctga
     3181 ccgtacctgt gcgactcatt ccagaggagc ttgaactttt caactggata aaaagctatt
     3241 gcattgagaa ggggatcccc tgtatgaaaa aaaatgatat tttacagcgt ggtccgaacc
     3301 gtttccggaa aaaggataaa atcaactggt tactggattt actgtatgaa caaaacagag
     3361 ttgtaccggt tattgaggga aaaacgttgt gtgtcgcacc taactttgac ctctgaacga
     3421 cttaatcgtt gagtaacata tagtctcctc tgctacttgc ggaggagact aatgccttta
     3481 ccacgtcagc gttgttgtat tagaaaactc tggtacaggt cagtcagtaa atataactgg
     3541 tgtaaccact atttactatt ggatagagtg ttgctcagga tattgtcggc tttagtctga
     3601 ggtgaggtgg gaccttcatt gttttcgtgt tcaataaata atttaggtta attagtatta
     3661 ttaatgggag ttatgttatc tgttatgtta tcttatctct ctctttcttt cgtaatatca
     3721 ctcctcttta tgctaagtat tctgtatgtg cgtaatacat atatctccat acactacagt
     3781 gtggtggtgt atggagttta gttatgtgta aattatgtta ctacactcat agcgtaatat
     3841 tgagaaagtt aatccactga atgtatgtct ggtttgtatt taacactagt tacgtaccag
     3901 aacactatta gagtatattg tctttagttc gtacgttata gctccgttac agtttatggg
     3961 taaatataat ctttttgtat gaaataatct tcttattgtg agattaaata tctgtaggct
     4021 ctctctgcag agtctctgtt tttacttaat ggtacggcaa tactaatagc gatgttgcct
     4081 gtggctattc gatagctgac ttttgtgaag atcagattac ttcctgtaag tgattgaatg
     4141 gtactttctt aaacttaatt ctatgaggtg tgtttttacc attgtaattc gtactggtcc
     4201 tcagtttaag agagtctcac tgtattctga agtattactc ccattactgt aatgaggtaa
     4261 tacgaaacag ttttatcagg ttgtctgtgc tgctggttac ctcgtttatt aatgccgcgg
     4321 tacggcgttt ctgtactctc agtatttatg taattccact ttcctgtcag ttttataaag
     4381 taaggactat aaaatgtata atgctaatcc caattacgaa atggatttca tgattctgaa
     4441 agacgtgaat gaacacatgg aaggtctgtt tcaacgtttt tctaaactgt tacctttcag
     4501 aattgatttt gcctacagga aagacacgcc cagttttggt catggctgta agcacagtat
     4561 gtgtatggaa atataccgct tacttagtga aacccaaacg atgctggcgg gatattactg
     4621 ggtaatggaa tatacacaaa acaagggact tcatattcat tttatcggtt acctggatgg
     4681 gcaacgtcac aagaactctt atcagatatc caggcaactg ggagatatct ggaggcggat
     4741 cacggaaggg gatggttatt ttcatctgtg ccgggctaaa gacaaatacc ctgtgtgtat
     4801 cgatcacgtg attcactata gtgataaatc cgcggtagac gacttacgtt acgccctcag
     4861 ctatctggct aaacaggatc agaaagaaca cggtattatt ctgggccgta gcaggcttcc
     4921 cgaaaaaagt aaccggggtc gaccacgaca taactgaatt acgggggctt atgtccgaac
     4981 tatcgtaatt tattgcgtgg atttgttgtt aacgtgtggc tttgtcctca gcgcagggga
     5041 ctatgacact tcatcttctg tggtgctacc ccgtggggca ggtacaggta acgcatatag
     5101 ttcataaatc tgacagtgat gaattagctg cactaagggc aaacataact tgaagctcat
     5161 gggattgaat ggcgacgtaa accgcagagc cctgtgcagt gcgttttcgt attatccacg
     5221 gatgagaaag ttgcgttatt tcgtcggttg tttcgtgggc gtgatgatgt atgggccctt
     5281 cgatgggaaa gcaaaaccag cggaaagggt attcgtctgc ctggctaacg aatggctgtt
     5341 gggaatatgc ggtaagcccc ggataaaatg tggggactgc gctcaccgtc agttgattcc
     5401 tgtatctgat ctcatcatct acctgaggta gtgtacaata tgccgatatc gaactgatac
     5461 ttaattaaaa tatctagagt gtgatatatc tctgagaact atcaatacga atattaaaaa
     5521 cacttccaga ataacagcaa tacgggggga tctccattac cctcctgtga ttgacaacgg
     5581 ggatatcatt acctgtttcc ctaacctgat ccccggagag atatctcgtt tccgtaactc
     5641 gctgaatgcg aaaattaatg cagatcaaca ccgtaaaaaa caacaaggca aacgcaccta
     5701 cgcaacgtcg gtttgtatta tctgggcgaa agaatatacg cagtccttta aatgtcatta
     5761 tcacgtctgc ctttttttaa caaagacgcc tgttaccacc tgggaaacta tgagcgctct
     5821 gacacactga gagggttaat taccggagcc tggtacagtg ccttagggtt ggaactggaa
     5881 gatcaccagg gattagttca tttccctgct aatggacgtt acatcctgga tattaacgat
     5941 cctgatttcc cagagcagta ccagcgctta ttggcacgtc tggattatct gacgaagctc
     6001 gacactaaag catcaggcca gcgtaacttt ggctacagcg gtttttgacc tattgtaact
     6061 gtaaggtgat ttattatgta tacagaatca aacattaagc tttcctgtaa cgccctctgg
     6121 caaaagtgtg tccttgcaac tctgtatcgg gctgctgcta cacatcctaa aattaaatta
     6181 ttacagttta aataatacat tgccctggta tgaataaggc aattccttct ctgatggctg
     6241 attttactgg tgaactgcat agactaattt ctgaa
//
LOCUS       DAZGBC010000224         6220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGBC010000224 DAZGBC010000000
VERSION     DAZGBC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.225"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1730
                     /locus_tag="ACHK1J_004618"
     CDS             <1..1730
                     /locus_tag="ACHK1J_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFO7913786.1"
                     /translation="EDDIEVINFMNGGKSRSEVIQSGDKSRPQSNTWNPFAFSTEAFT
                     AETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMA
                     KLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSET
                     FTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSM
                     ILARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSL
                     IMMAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGS
                     LQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFTVFQGDVVPSASVYIPDS
                     EKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSIIGVLTAKPSRKR
                     RKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREER
                     RVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAEKNKSQTINNGHPQKSYG
                     AQPTTDEKY"
     gene            complement(2190..4820)
                     /locus_tag="ACHK1J_004619"
     CDS             complement(2190..4820)
                     /locus_tag="ACHK1J_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO7913787.1"
                     /translation="MAKQAESAGSEKLLYNRLKQFDEPGLFHDNYQTPDYVQANLKDD
                     LRPYQHAALRYLHYTQRNPEEALLHYRHLLFHMATGAGKTMVMAGAILYLFKEYSYQN
                     FIFFVHTDAIIQKTRENLLNPQSPKYLFSQNLEIDGEKITIIPVDIFPQVPERNTIYL
                     RLSTIHKMHDELSSYRENGITSEDLKELPLVLLGDEAHHFNAGTKARGKAKNTSENEE
                     HTWERTIENILDLRPDNRLFEFTATINLANKDIWQKYRDKVVYQYDLKKFMSDGYSKK
                     VMLLEANQNDDDKMLDAVLLSQYRKLTAEDHGITDFKPVLLFKSNKIAVSKAKQAEFS
                     QLIAGLTPENVRRHLSNKKVRLSSDTSIWHKVIQRYDDSDLITVIGQIQEDFNDFNLL
                     NVNRSDLLEENPVLLNTLEDVDNPVRAVFAVAKINEGWDVLNLYDIVRISEQASSSKT
                     GTDSEAQLIGRGARYYPFVYDREKSFTRRFDNSTRDLSILEQLHYHTINEPAYIKMLH
                     ASLEQADIDAQHDGSGTIEHARLKEDFKKSAVYRTGKLFFNEVEDIESSSRTWETYSL
                     ETRFDIPYQTASEASLDNLAGTECVTKAVPLVLDERFYRKAMQRISFYSLDNLQRFFP
                     MLTSVRDFIRCEEYLGRLRITVTIPLSLDFSAIPAKEKLRLLETVLLRIADNVRRNDK
                     KVKGSYRFISQPVREVIKDYSLYIDQSVIMNQKITAAQTIGKKWYVYDNAILNQLEHR
                     LVKTLEKVMPKLRERYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILICKDDNSY
                     WQVFLEPKGDDRLQVDEWKENLLRSLNNDKRLILEEDASVRLIGIKFFADSKMDEFIK
                     DLEEKLNDGLPLEERSLSLF"
     gene            complement(4825..>6220)
                     /locus_tag="ACHK1J_004620"
     CDS             complement(4825..>6220)
                     /locus_tag="ACHK1J_004620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7913788.1"
                     /translation="ITSFESDNLIIKGNNLIALHSLKQRYAGKVKLIYIDPPYNTGTD
                     SFKYNDSFNHSTWLSFMKNRVEIAKELLSEDGSIWINLDDNEAHYLKVLMDQDTGLGF
                     VADVIWQKRTSPDARVPLGAGHDHILVYSLNPELFRTKINLIPLTQDQKNRYSNPDND
                     PNGPWVSSDFTAQGFRPNQMYEIKTPSGKSYYPPEGTCWKNIESEMIRQKEEGKMWFG
                     VNGDAMPRRKTYLKDVKGRMPWTWWPNSEVGHNQEAKKEINALNMDSVFSTPKPERLI
                     QRILHIGSNDGDLVMDFHLGSGTTAAVAHKMGRRYIGIEQMDYIHNITVPRMQKVIDG
                     EQGGISKDVGWQCGGSFLYVELMELNALFVNEIRKAQSSEELEKLFAVMKTEAHLNYQ
                     VALENVLSAEYEVDGIFRKVAFCELELHEQKKLLIEILDKNQLYVNASDMDDSDLNIS
                     ESDKAFTRSFYGME"
BASE COUNT         1632 a         1554 c         1338 g         1696 t
ORIGIN      
        1 gggaggatga tatcgaagtc atcaacttca tgaacggcgg aaaatcccgc agtgaagtca
       61 ttcagagtgg cgataaatcc cgcccacagt caaacacctg gaacccgttc gcattcagca
      121 ccgaagcctt caccgccgaa accatgcagt ccatgcttcc ccagaacgtt cagggcggtg
      181 aatggcagtc acgggcgata gccatgaaca aggcactggt ctttggcaca aaattctggt
      241 gcgtccggga aggtaaaacc atgtccctgc agatgttacg ggaacatatg acgctggaag
      301 ggatggcaaa actctattgc cgtggccttg atgaccagtg gccggaagaa gccatcgcac
      361 cactgcgaaa ctacctgcag gatgtcccgg gattcgatat gtcactggtg agaacgccgt
      421 cagcctggac tgaggagccc cgtaaacagc atgcttatct ctccggtcag ttctcggaaa
      481 cgttcacaac ctttgccgaa accttcgggg acgtctttgc ggcagatgcc ggcgacatcg
      541 atattcgcga cagtattcac agcgaccgca ttctgattgt gatgatccct gcactggaca
      601 catcggccca taccacgtcg gcactcggtc gtatgttcgt cacccagcag agcatgatcc
      661 tcgcccgtga cctgggctac cgcctggaag gcacagatgc acagaccctg gaagtcaaaa
      721 aatacaaagg cagcttcccc tatatctgta tccttgacga agtcggtgcc tactacacag
      781 accgcattgc tgtggaggcc acccaggtac gttcactgga gttttccctg ataatgatgg
      841 cgcaggatca ggagcgaatt gaagggcaga cctccgccac caacaccgcc acactgatgc
      901 agaacgccgg caccaaattc gccggacgca ttgtcagtga cgacaaaacg gcaagaacgg
      961 tgaaaaatgc cgccggtgaa gaggcacggg ccagaatggg cagcctgcag cgccacgatg
     1021 gtgtgatggg tgagtcctgg gtggacggta accagatcac catccagatg gaaagcaaaa
     1081 tcgacgtgca ggatttgatc agactgaatg ccggtgaatt ctttaccgtt ttccaggggg
     1141 atgtggtgcc gtcagcctct gtgtatattc cggacagcga gaaaagttgc gacagtgatc
     1201 cggtggtcat caaccgctat atttccgttg aagccccccg gctggagcgt ctccgccgtc
     1261 tcgtccccag gacagtgcag cgcaggctcc ccacaccgga gcatgtcagc agcatcatcg
     1321 gtgtactgac agcaaaacca tcccgtaaac gtcgtaaaaa ccgtacagag ccttaccgta
     1381 tcattgatac attccagcgc cggttggcaa cagcgcagac atcactggat ctgcttccgc
     1441 aatatgatac agatatcgaa tcccgggcaa acgaactgtg gaaaaaggct gtgcacacta
     1501 tcaataacac aaccagggaa gaacgccggg tatgttatat cacactgaat cgtccggaag
     1561 aggaacactc cggtcctgaa gatatacctt cagtgaaggc gattctgaat accctgctcc
     1621 ccctagagat actgttaccg gtcgctgaaa aaaataaatc gcagacaata aataacgggc
     1681 atccccagaa atcatatgga gcacaaccaa caactgacga aaaatactga aggaaaacat
     1741 atattttctg gtgaccatat ctgtcaatca tactgtatat gtccggggtt ttacagcaca
     1801 cgggataagg gccgcagacg cggcccttaa ttcagggcta accgggcata aacatatgga
     1861 cacgcatgta aatgatgagt taaaaaataa tccgtgcgtt tccggtgatg atattagttg
     1921 ttggtaacga aatctcatta gcgggccgga aacgcacttc tccctttcag ccggagtaac
     1981 cttcaaatat cactggcgga gacctgctag ttgaattcac ctcacaacct tgcattagcc
     2041 gaatcccctg tcagttcgtt gtaacacgcc agtggcatca ttttcacact atttatccgt
     2101 gacaatatcc cgataaacat cccaccacat gcggatacgg tttgtattca gtaaatacca
     2161 gaaacaactg ttttcctcca ctcacctggt cagaacaatg acagtgaacg ctcttccaga
     2221 ggaagaccat cattcagttt ttcctctaaa tctttaataa actcatccat tttgctgtct
     2281 gcaaaaaact ttatgccaat tagcctcaca ctggcatctt cttcaagaat cagtcgttta
     2341 tcattattca gagaccgaag cagattttct ttccattcat caacctgcag acgatcatct
     2401 cctttaggct ccagaaagac ctgccagtac gaattatcat ctttacagat cagtatcatg
     2461 atgaaatcag gcataaagcc acgtacacca ccaaactcag taagtttaaa gcgcgttgac
     2521 tgttcatcgt tacgcagaac ataaatatca tcataacgtt ccctgagctt cggcatgacc
     2581 ttttccagcg ttttcaccag acgatgttca agctgattca gtattgcatt atcgtataca
     2641 taccattttt tcccgatagt ctgagctgcc gtaattttct gattcattat cacggactgg
     2701 tcaatataca gactgtagtc cttgataacc tccctcactg gctgactgat gaagcgatac
     2761 gagcctttca ccttcttgtc attacgtctc acgttatctg ctatacgcag cagaacggtt
     2821 tccagcaggc gaagtttttc ttttgcaggt atggcagaga aatccagcga tagcggaatt
     2881 gtgactgtga ttctcagcct gcccagatac tcttcacaac gaatgaagtc acggacagag
     2941 gtcagcatcg gaaaaaaacg ctgcagatta tcaagtgaat aaaagctaat cctctgcatc
     3001 gccttgcggt aaaagcgctc atccagtacc agcggtactg cttttgtcac acattcagtt
     3061 ccagccaggt tatccagtga tgcctcactg gctgtctgat acggtatatc aaaacgagtt
     3121 tccagcgagt atgtttccca ggtacgactg ctgctctcaa tatcctccac ttcattgaag
     3181 aacagtttcc ccgtacggta cacggctgac tttttaaagt cctctttcag tctggcatgc
     3241 tcaatagtgc cgctgccatc atgctgtgca tcaatgtctg cctgctcaag ggaagcgtgc
     3301 aacattttta tatacgccgg ttcattgatg gtgtgatagt gcagttgttc aagtatgctt
     3361 aaatcccttg tactgttatc gaagcgacga gtgaagctct tctccctgtc ataaacaaac
     3421 ggataatagc gagcaccacg accaataagt tgcgcctcac tgtccgttcc tgtcttacta
     3481 ctgctcgcct gttcgctgat gcgaacaata tcgtagaggt tgagaacatc ccagccttca
     3541 ttgattttcg ccacagcaaa gacagcccgg acagggttgt cgacgtcttc cagcgtattc
     3601 agcaacaccg gattttcttc cagcaggtca gacctgttga cattcagaag attaaagtcg
     3661 ttaaagtcct cctggatctg tccgataacc gtgattaaat cactgtcatc gtaccgctgt
     3721 atcactttat gccagatact ggtgtcagag cttagccgga cttttttatt agacagatgg
     3781 cgtctgacgt tctcaggtgt cagcccggca atcagttgcg aaaactccgc ttgtttcgct
     3841 ttcgatacag caattttatt cgacttgaaa agcaggaccg gcttgaagtc agtgataccg
     3901 tgatcctccg ccgtcagctt gcgatactgg cttaacaaca cggcatcaag cattttgtcg
     3961 tcgtcattct ggttggcctc cagcaacata acttttttcg agtaaccgtc tgacataaac
     4021 ttcttcaggt catactgata cacaactttg tcacggtatt tctgccagat atctttgttc
     4081 gcaagattaa tagtcgcggt gaattcgaac agacggttat ccggtctcag gtcgagtatg
     4141 ttctcaatcg tacgctccca tgtatgttcc tcgttttcag atgtgttttt tgccttgcct
     4201 ctggctttag taccggcatt gaagtgatgc gcctcatcac caagcaacac aagaggaagc
     4261 tctttcaaat cctcactggt gataccattc tcacggtagc tactcagttc atcgtgcatc
     4321 ttatgaatgg tcgagagcct gaggtaaatc gtgttgcgtt caggcacctg cgggaatata
     4381 tctacaggga tgatagtgat tttttccccg tcaatttcca ggttctgact gaacaggtac
     4441 tttggcgatt gtgggttaag cagattctcc cgggttttct gaataatcgc gtcggtatgc
     4501 acgaaaaaga tgaagttctg gtaactgtac tctttaaaca ggtaaagtat cgctccagcc
     4561 attaccattg tttttcccgc tccagtggcc atatggaaca acagatgacg gtaatgcagt
     4621 aatgcctctt ccggattacg ctgcgtgtaa tgaagataac gcaacgccgc gtgttgatac
     4681 ggtcgtaaat catccttcag gttcgcctga acataatccg gtgtctgata gttgtcatgg
     4741 aacaaccctg gttcatcaaa ctgcttcagc cggttataca acagcttttc gctgccagcg
     4801 gactctgctt gcttagccat atagttactc cataccatag aaactgcggg taaaagcttt
     4861 gtcgctctct gaaatattca gatcgctgtc atccatatca gaagcgttca catagagctg
     4921 atttttatcg agaatttcaa tcaggagctt cttctgctca tgcagttcca gttcacagaa
     4981 tgcgacttta cggaagatgc cgtccacttc atattcagcg gaaagcacgt tctcaagtgc
     5041 gacctggtag ttgagatgcg cctctgtttt catcactgca aagagctttt cgagttcttc
     5101 actcgactgc gctttccgaa tttcattcac gaagagggca ttaagctcca tcagttcgac
     5161 atagagaaaa ctgccgccgc actgccagcc aacatctttt gaaatgccgc cttgttctcc
     5221 atcaataacc ttttgcatac gtggaacagt aatgttatgg atgtaatcca tctgctcaat
     5281 accgatatag cgacgcccca ttttgtgggc aacagcggcg gttgttccac tgccaagatg
     5341 gaagtccata accagatctc cgtcattcga tccaatatga aggattcttt gtattaatct
     5401 ctctggcttt ggtgtcgaaa aaacactatc catattaagg gcattaattt cttttttggc
     5461 ttcttgatta tgccctactt cagaatttgg ccaccacgtc cagggcattc gtcctttaac
     5521 atcttttaag tatgttttac gtctgggcat agcgtcacca tttacaccaa accacatttt
     5581 tccttcttct ttttgtctta tcatctcaga ctcaatattt ttccagcacg ttccttctgg
     5641 tgggtagtat gatttacctg aaggtgtttt tatttcatac atctgattag gtctgaaacc
     5701 ttgcgcagta aagtctgatg acacccatgg tccattaggg tcattgtctg ggttgctata
     5761 tctatttttc tgatcttgag ttaaaggtat taaatttatt ttagttctaa acaattctgg
     5821 atttaaagaa tagacaagaa tatgatcatg cccggcccct aatggaaccc ttgcatctgg
     5881 tgatgttctt ttttgccaaa taacatcagc cacaaaaccc aacccagtat cttgatccat
     5941 aagcacttta agatagtgcg cttcattatc atctaaatta atccagatac tgccatcttc
     6001 tgaaagaagc tcttttgcta tttccactct atttttcata aaggacaacc acgttgagtg
     6061 gttaaaactg tcgttatatt taaagctatc agttccagta ttatacggtg gatcaatata
     6121 aatcagcttt acttttcctg catagcgttg tttcagagag tgaagtgcta tcaggttgtt
     6181 tccttttatt attaaattat cactttcgaa agaggtgata
//
LOCUS       DAZGBC010000225         6215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGBC010000225 DAZGBC010000000
VERSION     DAZGBC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.226"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..1497
                     /locus_tag="ACHK1J_004621"
     CDS             37..1497
                     /locus_tag="ACHK1J_004621"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO7913789.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLFLLAHGAKATVAKYSVK"
     gene            complement(1532..1735)
                     /gene="ydfZ"
                     /locus_tag="ACHK1J_004622"
     CDS             complement(1532..1735)
                     /gene="ydfZ"
                     /locus_tag="ACHK1J_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO7913790.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1912..2598)
                     /gene="rspR"
                     /locus_tag="ACHK1J_004623"
                     /gene_synonym="ydfH"
     CDS             complement(1912..2598)
                     /gene="rspR"
                     /locus_tag="ACHK1J_004623"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO7913791.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2687..3433)
                     /gene="ydfG"
                     /locus_tag="ACHK1J_004624"
     CDS             complement(2687..3433)
                     /gene="ydfG"
                     /locus_tag="ACHK1J_004624"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO7913792.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3570..5615
                     /gene="dcp"
                     /locus_tag="ACHK1J_004625"
     CDS             3570..5615
                     /gene="dcp"
                     /locus_tag="ACHK1J_004625"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO7913793.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGSLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWGFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDEVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5660..6178)
                     /locus_tag="ACHK1J_004626"
     CDS             complement(5660..6178)
                     /locus_tag="ACHK1J_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO7913794.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1536 a         1489 c         1599 g         1591 t
ORIGIN      
        1 catgtagctt gccatattta tatttaagga atagttatgg gaaataattt gttatcagca
       61 aaagcgacgc tccctgttta tgatcgtaat aacttggccc caagaattgt tcatttaggc
      121 tttggtgcat ttcaccgtgc gcatcagggc gtgtatgccg atattcttgc tacagaacat
      181 ttcagtgact ggggatatta tgaggtcaac ttaatcggtg gcgaacagca aattgccgat
      241 ttacaacagc aagataatct ttataccgtt gcggaaatgt cggccgatgc gtggacggca
      301 cgcgtcgttg gcgtcgttaa aaaagccttg cacgtacaga tggatggctt agaaaccgtg
      361 ttggctgcga tgtgtgaacc gcaaatcgcg attgtctctc tgacaatcac cgaaaaaggg
      421 tatttccact ctccggcgac cggacagtta atgctcgatc acccgatggt cgctgccgac
      481 gtgcaaaatc cccaccagcc gaaaacagca acaggggtga ttgttgaggc gctggctcgc
      541 cgtaaagcgg caggacttcc cgcatttacc gtcatgtcat gtgacaacat gccagaaaac
      601 ggtcatgtta tgcgtgacgt tgtcacttca tacgcacaag ccgttgatgt aaaactggca
      661 caatggatcg aagataacgt gactttccca tcaacaatgg tggaccgtat tgtgcccgca
      721 gtgacagagg atacgctagc gaaaatcgaa cagcttaccg gtgtgcgcga tcctgctggc
      781 gttgcctgtg aacctttccg ccagtgggta atcgaagata actttgttgc cggacgtccg
      841 gaatgggaaa aagcgggagc cgaactggtt agtgatgtgc tgccttatga agagatgaag
      901 ttgcgcatgc tcaacggcag tcattcattc ctggcgtatt tgggttatct tgccggatat
      961 cagcacatta atgactgtat ggaagatgaa cattatcgtt atgcggcgta tgccttgatg
     1021 ttgcaggaac aagcgccgac gctgaaagtg cagggcgttg atttgcaaga ttacgctaac
     1081 cgattaattg cacgctatag caacccggcg ttacgccatc gaacctggca gattgcgatg
     1141 gatggcagcc agaaattgcc acagcggatg ttggattctg ttcgctggca tctggcgcat
     1201 gacagcaagt tcgatctgct ggcgctgggc gtcgcgggtt ggatgcgtta tgtcggtggt
     1261 gttgatgaac agggaaatcc gattgaaatc agtgacccac tgttacctgt tattcagaag
     1321 gctgtacaaa gtagtgccga agggaaagcg cgcgtccagt cattgctggc gattaaggcg
     1381 atctttggtg atgatttgcc agacaatagc ttgtttactg caaaagtgac ggaagcgtac
     1441 ttgtttttat tagcgcatgg tgcgaaagcg accgtggcga aatattccgt gaagtaaaaa
     1501 agcaatgccg ccatcggcgg cattcacacg cttagttcat gccgagacga atcaggtcca
     1561 gtggtgccag tttctcttca caaccttcaa caactacggt ttttccgcgg cggatttgct
     1621 cagcggtcag accttcagta tcaatcgaca ggatcttgcc agtgtgaccg gtgccgctaa
     1681 ccataacacg gctgccagtg gtgattgcgt tacggttacg atcgtaggtc gtcataggtg
     1741 ttttctcctt tctgatttac agtcgaagcc cacgtaacgg gcgcaatata aatactcctg
     1801 tcgagtgaaa tattttttgt ttttgatcaa gctcacatct tttttgatgc atgcatgagc
     1861 cataaggcgg gggaaaagac tctcaccccg gatgggatga gaggaaatga attactcttc
     1921 gctaaaccag tcgctgtttt cctggcggat ttggcgcacg gattcgctga tttcctgcaa
     1981 atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt ttttgcaggg cagagaaaat
     2041 atcaagatgc tggcgtaaca gcatttctgg tggagaaacg tggtcgaaac tcatatagcg
     2101 cacgcgatca acggtcgctt tcaggttctc aatggtatcc cacgccagtt gacagtcggc
     2161 aatctgcgtc aggagttgat ggaagttgtc atcaagttca aaaaaatcat ccagttgctt
     2221 gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc agttggtagc actggctttc
     2281 ggtaatcatg ctcgccgccc gacgcgccac cgcgcactca atggcctgac ggataaaact
     2341 gccgttgcgc acctgggcca tggaaatttt gttgacgtag ctgccacgct gcggacgaat
     2401 ttgaatcagg ccgttttccg ccagtttaat aaaggcttca cgaaccggct ggcgtgacac
     2461 attgaaacga acagaaactt ctttttccga caacggtgtg cctggggcaa tcaggcaatg
     2521 gacaatgtcg cgacgaagga tacgataaat ctgctgattg acaggctgtg tgggattaag
     2581 ttgcgtttcg acggtcattc gttcttgctt gtgagtgagt taactgcatg agtctaccac
     2641 ttttttgtga caagcaataa cccggcagtt acgccgggta taaaaattac tgacggtgga
     2701 cattcagtcc ggcatagctt tgggtaaccg gcatcatttc cagggtattg atattgacgt
     2761 gagcaggcag cgttgacacc caccagacgg cttcgctgac atcctctggc gtcaatgcaa
     2821 cggtattttg ataggttttt tccgctttac cgtcatcgcc tttaaagcgg acattggaaa
     2881 actcggtgcc acccaccaga cccggttcga tgtcggtgac gcgcaccgcc gtaccatgca
     2941 gatccgtacg cagattcagg ctaaactgac gaacaaacgc tttcgtcgca ccgtaaacgt
     3001 taccaccggc atacggccag ctacctgccg ttgagccaat gttaataata tgaccatgat
     3061 tacgttcaac catacccggt aagacggcgc gcgtcatata taccaggcct ttattgttgg
     3121 tatcaatcat cgtttcccag tcttcaacgc tggctttatg cgcaggctcc atgcccaacg
     3181 ccaggccggc attatttacc aggatatcaa tattgctcca ctcggcaggg agcgatgcca
     3241 gcatctcttc aatagcggca cggttgcgaa cgtccagttg ggcgatatac agattatctc
     3301 ccagttcgtc ttttaactcc tgcaaccgtt cctggcgacg gccagtggcg ataactttat
     3361 gcccttgttg aataaaacga cgagtaatgc attcaccaaa acctgccgtt gctccagtta
     3421 ctaaaacgat catctcactg ttcctcaacg cttttgtgtg tcaataacat agcacgggat
     3481 gcttaagagc gggtaatact ggtttgcaag attgcactcc gttatcagta agcctacgct
     3541 atggaacacc tcgcgttcag gagaagaaaa tgacaacaat gaatcctttc cttgtgcaaa
     3601 gcacactgcc gtatctggct ccccattttg atcaaattgc caatcatcac tatcgcccgg
     3661 cattcgatga gggaatgctg caaaagcggg cagaaattgc tgccatcgcg cttaacccgc
     3721 aaacacctga tttcaacaat actattctgg cactggaaca aagcggagaa ttacttaccc
     3781 gcgttaccag cgtctttttt gccatgactg cggcgcatac caatgatgaa ttacagcgtc
     3841 ttgacgagca gttttccgct gaactggcgg aactggctaa tgatatctat ctgaacggtg
     3901 aattattcgc gcgggtagat gctgtctggc aacgccgtga atctctgggg cttgatagtg
     3961 aatccatccg cctggtggag gtgattcatc aacgttttgt cctagccgga gccaaacttg
     4021 cacaagctga taaagcaaaa ttaaaagtac tgaatacaga agctgcgaca ctgaccagtc
     4081 agtttaacca gcggttactg gcagcaaata aatccggcag tctggttgtg aacgatatcg
     4141 cgcagctggc aggaatgagt gagcaagaga ttgcgctggc ggcagaggcg gctcgcgaga
     4201 aaggtctgga taacaaatgg ctgattccgc tgctgaatac cacccaacaa ccggcgcttg
     4261 ccgaaatgcg cgatcgtgcg acgcgtgaaa aactgtttat tgcgggctgg acgcgagcgg
     4321 aaaaaaatga tgccaatgat acccgcgcta tcattcaacg tctggtggag atccgtgcac
     4381 aacaggctac attactcggt tttcctcatt atgccgcatg gaaaatcgcc gatcagatgg
     4441 caaaaacacc tgaagcagca cttaacttta tgcgggaaat tgttccagcg gcgcgtcaac
     4501 gtgcgagcga tgaattagcc tccatacagg cggttatcga taagcagcag ggcgggttta
     4561 gcgcgcagcc gtgggactgg ggattttatg ccgaacaggt ccggcgggag aaatttgatc
     4621 ttgatgaggc gcagctcaag ccatattttg aattaaacac ggtgttgaat gaaggtgtat
     4681 tctggaccgc gaatcagctc ttcggtatta agtttgtcga acgttttgat attcctgtct
     4741 accatcctga cgttcgggtg tgggaaattt ttgatcataa tggcgtgggg ctggcgttat
     4801 tttacggtga tttcttcgcc cgtgattcaa aaagcggcgg tgcatggatg ggcaattttg
     4861 ttgagcaatc aacgcttaat gaaacgcatc cggtaattta taacgtctgc aattatcaga
     4921 aacccgctgc cggtgagcct gcgttgttac tctgggatga agtcataacc ttattccatg
     4981 aatttggtca tacgctacac ggcctctttg cccgccagcg ttatgccacg ctctccggta
     5041 ccaacacgcc gcgtgatttt gtcgaatttc cgtcgcaaat caacgaacac tgggcaacgc
     5101 atccgcaggt attcgctcgc tacgcccggc attatcagag cggggcagca atgcctgacg
     5161 aactgcaaca gaaaatgcgt aatgccagcc tgttcaacaa agggtatgag atgagcgaac
     5221 tgcttagcgc cgcacttctc gatatgcgct ggcattgcct ggaagaaaac gaagcaatgc
     5281 aggatgtcga tgatttcgaa ttgcgggcgc tggtggcgga aaatatggat cttcctgcta
     5341 taccgccacg ctatcgcagc agttatttcg cccatatttt tggtggcgga tatgccgcag
     5401 gttattacgc ttatctgtgg acgcaaatgt tggccgatga tggttatcag tggtttgttg
     5461 agcagggcgg attaacgcgt gaaaatgggc tgcgttttcg cgaggcgatc ctttccagag
     5521 gtaacagcga agatctggaa cgcctgtatc gacaatggcg cggtaaggct cctcagatta
     5581 tgccgatgct gcaacatcgt ggcttgaaca tataaacacc accaatggct gtcctggaga
     5641 tatcgccagg ccagcctggt tatagcagta attgcagcag ctgggcgagg gcaaatctca
     5701 ccgctaaagc taataccgtt tcgcagtcgc cagcaaaatg cataaccgca gtgtagttct
     5761 ggcctttaat atgccacgcg aaccagacgg tgcccgctgg cgtaccatct tcaccgccct
     5821 ccggtccgcc gtagccggta atggcaatac tgacatcagc atctgcacgc tctatggcac
     5881 cggttgccat ttctgctgcc actttctcac tcaccgcaga atatcgttca agagattgct
     5941 ggcttacgct gaggattttc atctttgcct gatcggtgaa agtaacaaag cctgcaccgt
     6001 aaaatttggg tgtatcttca gctgcacaca gggcgctagc caacttaccg ccggtgcatg
     6061 attcggcggt agttaagcgt aattgatccg cgataagtct ttgactcaac gcggatgtca
     6121 ggttttcaat agtgtcggta tctgcgagtt gtacaatttt atctttatta atgttcactg
     6181 ttttttgtcc acgctattat atgtaaaggt gattt
//
LOCUS       DAZGBC010000226         6180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGBC010000226 DAZGBC010000000
VERSION     DAZGBC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.227"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..337)
                     /gene="nudI"
                     /locus_tag="ACHK1J_004627"
     CDS             complement(<1..337)
                     /gene="nudI"
                     /locus_tag="ACHK1J_004627"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO7913795.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQ"
     gene            complement(339..1046)
                     /locus_tag="ACHK1J_004628"
     CDS             complement(339..1046)
                     /locus_tag="ACHK1J_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFO7913796.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYDAYMVLWSRINELLTEPDIIAFINYPIAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            complement(1043..1909)
                     /locus_tag="ACHK1J_004629"
     CDS             complement(1043..1909)
                     /locus_tag="ACHK1J_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO7913797.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKEKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            2394..2645
                     /locus_tag="ACHK1J_004630"
     CDS             2394..2645
                     /locus_tag="ACHK1J_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7913798.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            2642..2929
                     /locus_tag="ACHK1J_004631"
     CDS             2642..2929
                     /locus_tag="ACHK1J_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7913799.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            3097..3501
                     /locus_tag="ACHK1J_004632"
     CDS             3097..3501
                     /locus_tag="ACHK1J_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFO7913800.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            3810..5294
                     /locus_tag="ACHK1J_004633"
     CDS             3810..5294
                     /locus_tag="ACHK1J_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7913801.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(5515..6129)
                     /locus_tag="ACHK1J_004634"
     CDS             complement(5515..6129)
                     /locus_tag="ACHK1J_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7913802.1"
                     /translation="MGKDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATES
                     VSSGISSPAVETHIPEAPARKKKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRA
                     KGGGFLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRY
                     NLDGQPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
BASE COUNT         1772 a         1288 c         1294 g         1826 t
ORIGIN      
        1 cctgaaactc ctcattaaat gtaactgtac ggtttgtact gatacagtcg aatattaggt
       61 aaatcatgta aatgtcctcg tgggtaccat ccgggtacgt ttttgtacgg atatcatcac
      121 gaaaggccca gggttttacg tcagtgatat gcagttcttt tcccagttct tcctttattt
      181 cacgcctcaa tgctgtttcc atagtctctc cgggttccat tcctccaccg ggcagtgccc
      241 attggcccgg gaatactccg cgatcagagg ccattttaca aagtagaaac tcaccttcat
      301 tttgtatcac agggcacact attactctct ggcgcatttt atgtcctcaa catcatggct
      361 ttttggataa ccttatcaga aaaatcatca atgtcatccg ggtgagcaca taccataatc
      421 cgctcacgaa ggtgtaacgg aagattatca gtaaaatgtt gatattccgc atgccatcct
      481 gtgatccatt ttatcttcat ttcctcatct ggaaattctt caggccgtac accatgaatt
      541 tcatcatcat tgagatgcat gagtgaatgt gcgatgggat aattaatgaa tgctataatg
      601 tctggctctg taagtaattc atttattcta ctccagagca ccatgtatgc atcatattgt
      661 gctttattta tatattcgga cttcagcatg aagttgataa aaatataatc ctcaatatgg
      721 gatctctcca taataaggtt aaacccttta tccagccaag attttatcag taatgctcgt
      781 tgtatcataa aattaagctg gattagataa ccgtagttac tggtatcgtc aaaaaggtaa
      841 ggaaggagag tgtggtggac acttttttca tctatcgcga cagtgtctgg atctacctta
      901 aataactcct ttgacatgcg tctcagaaga gtacttttac ctacaccgga attacccgac
      961 agacatatat acagaggatt tcgagtttta ttcttctctt ttttatctgc tgaccaagga
     1021 tccatataca agtaattata tctcatactg taaactccat tttgtccaac caaaagcggt
     1081 tattcttaca tttttgcatg atttcattat ttccgaaaat tgcactgatt atatgatctt
     1141 gtgaatctgt cagcatattt cccaacagag tatagttttc tccatcaggg ataataatgt
     1201 caaaattggg ttccataaca acattggctt tgtcccgttc ttcgaaagtt gcccaactaa
     1261 tgggcatgtt agggtgttgt ttttttagca ttaccatttt ctctttaaat atatccgtat
     1321 tgatttccag tttatctctt ttttcttttc cgactcctag aggacagaac tggtaaaatc
     1381 gccaagcttg gatcttatag ggcagagaat caacccagca aacaatatca ctgagatgat
     1441 taatgttaga ctgagatacg acggtcgtga gttttatatt aattcgatta ttattttggg
     1501 gtaaccatgt taatatattt ttgataaccc tgtgttggtt actcatttta tcactgcgaa
     1561 tttctgaatt gacaagactg tcaattccat caattggtat tcctacccag tctaacaccg
     1621 gatttaaccg agcatgatcc ataataaatc gatgcccgac cgtggagaga tatgtcataa
     1681 aacccagttg cttagcgtat tcaatcagat caatgaaatc actccgggat gtaggttccc
     1741 ctccggtaaa accgatacga cggaatccca ttttatacat ccgctcaatt acattacgag
     1801 cactagcaag agaagagtcc tgaattccag gtgtacgaaa acacagatca catcctaggt
     1861 tacaatgtga agttattctg aaatctaaaa gaagggtgct aatatccata acaagtcctc
     1921 tgactatgat acatcaataa ccattaaaac ataatggtta ttgcagacca tgacaaagcg
     1981 caaccttcgg cttacctagg atataatatc tgccagtaat cagagcagtg ggttgcgcaa
     2041 ggaactcatg aatgatgagt tccggtaatc gcattactgg aagcccatga tcggagggac
     2101 gctatcagtc acaccagtga aaattgtgca cagaagcatt ttcatcactg acaggccgta
     2161 tgcctcacaa gacgggagat aactggagaa tatttagttc ttctaccggc ttacgccgat
     2221 tttactcctg tgttatatgt aaccactgtg tgccaccttc catgcgctgg tgcggcatca
     2281 gagtaaaaaa tcagcattaa atttatttgc aaatgtatga gtaatacaga cttgttaacg
     2341 ggtcctttca gatgtatctt taagacttat taaacagtct taaaaggagc attatgccta
     2401 acatcattct gtctgacatc agtgcaagtg tcagtgaact gaaaaaaaat ccgatggcga
     2461 cagtcggtgc tggtgatggt tatccggtcg ccattctgaa tcgcaatcag ccggcattct
     2521 actgtgttcc tgctgaactg tatgaaagga tgcttgatgc tctggatgac caggagctgg
     2581 tgaaactggt tactgaacgt agcagtcaac ctctgcatga tgttgacctg gacagttatt
     2641 catgacttat acgataaaat tcagggatga tgcgcttaaa gaatggctga aactggataa
     2701 gaccattcaa caacagtttg tgaaaaaact aaaaaaatgt agcaaaaatc ctcatatact
     2761 gtctgcaaaa ctacgtgggc taaaggactg ctacaaaatt aaacttcgtg catcgggatt
     2821 tcgtctggtt tatcaggtga ttgatgatat gttaattatt gcggttgttg cggtaggtaa
     2881 acgagaacgt agtaacgttt attatctcgc cagtgagaga atgagatagt aacataaata
     2941 gcctttcccg cagataactc tcagaattgt tattctctct gtggcctcgc ctgaggcgga
     3001 aaaagaaaag ttttgttgag agaatgattt tacctgccgg gatgagtctg gggttattta
     3061 tattaccaga caaattgaaa cggagggaca tgtttgatga caatacttgt gacggcacag
     3121 gctcaactca tagttggtga ggctatggcg ataaaaagtt tagctcctga tggaatgttg
     3181 gccgctgtat ttgaagacga cgggaatacc ggatatttct atgcacttga tgagtccgtt
     3241 gaaggtaacc ctgtcaggga tgctgtgcat atatataacg ttgaagacat ttcagatgct
     3301 cacatcccct ctgatgtgaa gattggctgg tctgaggatt gcctgaagtg tgttctgctg
     3361 ataaacggat atccacatgg ggtattcgat tttgagggga agaacggcta ctgtcgaagt
     3421 ggattcccgc caccggttaa tcatgaatgg tctgttcagg ggcattcatg ggatgattac
     3481 gtcgataatt tcttccggtg attgaaccga aaaattacct gacggacaag tctggggccg
     3541 aatcgtgatg attcatcatg gccgtttata gttcagcagt cttgttcttt tgctgtcagt
     3601 gggataccag gaaaataacc gattgttacc agacagaata accatcataa gacgtcacaa
     3661 gacggtgacg cttacatagt tccggggagt taaacaatat accctgactt tgtaatcatt
     3721 tgaattatat ccgtaacgtg taacttcctg ccgtaacacc ggatttaaga tattcaaact
     3781 ttatctttat accgatatta agagacacaa tgcgaaaaat aattgctcat ttcaagatag
     3841 ttttaacctt acttctgccc gcaacagtat ctgcccagtc gatagagtgg caatcctgta
     3901 tgaccactcc attcagtaac tggtttggtg aggaaaaacc gtctcctgac ttactatgtg
     3961 gttatttgtc tgttccatta aaatatacag acacaggcgg agatagttct tctgtaaaaa
     4021 gacagcaggt aaaactggcg ctgacaaaat tgccagcaaa aagcaggcat aaagggagta
     4081 tcattataat aagtggtgga ccaggattac ctggcattaa tccttatatt aactttgact
     4141 ggccggtaac aaatcttcgc gaatcatggg atattattgg ctttgatcca cggggagtcg
     4201 gccagtccat tcctgcaata aactgccagc aatcagatac agggattcag gaaaacataa
     4261 ctgaaaagca acgggtatta aataaaatta acagctgtat ccataatacc ggaactgaag
     4321 ttattcgcca tattggttct aacgaggcgg tatatgatat tgatcgtatc aggcaagccc
     4381 tgggtgacga gcaactgaca gctgtggcat attcgtatgg aacacaaatt gcagccttat
     4441 atgcagaacg tttcccatcc agcatcagat ctatcgttct tgatggggtc gtcgatattg
     4501 atgacctggg ggacaacttc acatggcaac tcaaacaagc acagagttat caggaaacgt
     4561 tcgatcgctt tgcatcctgg tgtgcacgta caaaaagttg cccactttct tcagtcagag
     4621 ataaggcaat aactcagttc catgagctgt tatcaaaatt gcatcacaag cctttattag
     4681 acagcaaggg agaaaatata tcttcagatg aactcatatt attaacaaca gaccttttgt
     4741 tatggcgttc atcatggcca acccttgcaa ctgccatacg tcagttctct caggggattg
     4801 tcagtaatga aattgaaact gcgctcagta ctcctatagc ctcagaagag tcaagtgatg
     4861 ctctgggggt aatcctttgt gttgatcagg ggggggagca attaacccca gaagagcgaa
     4921 aatcccgaaa agacgctctt gcgaatgcct tcccggctat taactttgac aatggacgtt
     4981 ccgattcacc tgatttttgt gaattatggc caatacatag caacctgaat aaaacccggc
     5041 tgaaaaatac agttctgccc tctggtttac tgtttgtagc acacaaatac gatccaacaa
     5101 caccatggat taatgctcgt aagatggcag agaaattttc cagcccgttg ctgacaataa
     5161 atggtgatgg gcatacatta gctcttaccg gagtcaattt atgtgtagat aaggccgttg
     5221 ttcgtcatct tatcacccct atgaaaatgg gaaatatatc ctgtccagga aatgctgaaa
     5281 tagatataaa atagtttaaa aattgcccgc catttgctcc tgataccgga catattttgc
     5341 attgggagca aaatgcgttc agtgcgatca cggcaccaga aacgtcaaac tggatacacg
     5401 taaaaaatgt cgcgatcgcg caaaatcaag ccattactct cgggagtgct taaataatac
     5461 gcacaatata caacgctctc ttctctacag tctgtttcct gtaatatcct gcggttattt
     5521 tgatttctca gaaatatttt cctgccgggc cttcatactc agccacctct gctctttcgc
     5581 cgccagccag cgcctggaat tttgagcatc acattccgat acttccccct ctggttgacc
     5641 atccaggtta taacggggag ttcctgcaac agaggttctc tgcaggtaga tccggcgact
     5701 ggtaatgcag gatactgcac aattccattc gtccagggtc agcgctgttt ccggatgtgc
     5761 tgccagatat gtagtaatgt cacgggttat gcctgttttc agaggcagaa aaccgcctcc
     5821 tttggcacga agttgaggaa acagcgcttt caccttctcc agacaatcgt gagtgaactc
     5881 actggtccag tgggatggtc ttggaaagcg ttgtcttttc tttttcttct tcttcttcct
     5941 ggctggcgct tcggggatat gagtttcgac ggcaggtgaa ctgattccgg aggagactga
     6001 ttctgtcgcc tgttcagtaa gtgcaggcgc gataacatct tttttttcgg ctaaggaagg
     6061 cggtgaaaaa gagcgacgtt ttttcaccac aatgacggga tttttctttt cttccagatc
     6121 ttttcccata aatacctctg cttcagaatt tattttttaa taatatcaca aattataaat
//
LOCUS       DAZGBC010000227         6166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGBC010000227 DAZGBC010000000
VERSION     DAZGBC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.228"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..333
                     /locus_tag="ACHK1J_004635"
     CDS             118..333
                     /locus_tag="ACHK1J_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7913803.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            507..764
                     /locus_tag="ACHK1J_004636"
     CDS             507..764
                     /locus_tag="ACHK1J_004636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913804.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMRTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            746..937
                     /locus_tag="ACHK1J_004637"
     CDS             746..937
                     /locus_tag="ACHK1J_004637"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7913805.1"
                     /translation="MCKRLLAIPGFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1079..2098
                     /locus_tag="ACHK1J_004638"
     CDS             1079..2098
                     /locus_tag="ACHK1J_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO7913806.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWVRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKIRLLGISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2855..3034
                     /locus_tag="ACHK1J_004639"
                     /pseudo
     CDS             2855..3034
                     /locus_tag="ACHK1J_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3209..5179
                     /locus_tag="ACHK1J_004640"
     CDS             3209..5179
                     /locus_tag="ACHK1J_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO7913807.1"
                     /translation="MYMNVIRTVICTLIILPVGLQTATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLEPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNVASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYAPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5198..5989
                     /locus_tag="ACHK1J_004641"
     CDS             5198..5989
                     /locus_tag="ACHK1J_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO7913808.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1831 a         1373 c         1501 g         1461 t
ORIGIN      
        1 aatagatata gtaacaggaa catcagtaag cgatgagatg gtcttccccc tccttcacga
       61 caacctttaa ttacgtcatg atggataaac tttaaccaca aaagtaagag ggataccatg
      121 tctcaacaga aaatcaccgg catcgatctg gctaaaacga acttttatct tttcagtatc
      181 aatgctcatg gcaaacccgc aggaaggatc aaactttctc gcaatcaact gcttaactgg
      241 ctggtgcaac agcccaaaat gactgttgcg atggaggctc gcgggcatcc catcactggg
      301 ccagggaaat caggaaactc gaccatgatg taatatttct tccggcgcaa catgttaaag
      361 cttatcagcg acgccagaaa aatgattatg acgacgctca ggcaatagca gaggcctgcc
      421 aacatggaac tatacgcccc gtaccaataa aaactctgga gtaacaggac gtccagactt
      481 ttttgaaaat gcgccggttg gtttcgatgg aaaggacaca actgataaat cacgttcgtg
      541 gacttcttgc tgaatatggc attgtgttca gtaaaggcgc aacagaatta cgccaaaaat
      601 taccggcatt acttgaggat gcggaaaacg aactcactga taccatgagg acgttattgc
      661 atcggcagta tatacggttg attacactag ataatgaact ggagtggtat gattctgaac
      721 tgaaaaatat gtccgccaag atcctatgtg caaacgcttg ttagccattc ctggattcag
      781 ccctctggtt agtcaggcaa taaaaagctg gatgggggat ggaaaacagt ttagacgagg
      841 aagagatgca tctgcgactc tgggactggt tccccgacag ttcagcacag gtgggaagca
      901 ggttcttctg ggtataacaa aatttggaga aatgtaatga acacatccca ccccattaag
      961 tcaggtgctc tgtgatggat gaagggaaat actggaaggt gtggagcctc gcagatttcc
     1021 ggcatcatag tgcccaatac aggatgcata aagctccctc agactggaga ctccacaaat
     1081 gaaatataca ccggttggcg ttgatatcgc aaaacatctg attcaggttc atttcatcga
     1141 cgagcacaca ggtgaagtga ttgataagca actacgtcgt caggattttc tgatgttctt
     1201 ctgtaatcgg gaaccctgcc tgattggtat ggaggcctgt agaggctctc agcactgggt
     1261 gcgtgaactg acaaagctgg ggcataaagt ccggttgtta caggcccact ttgttaaggc
     1321 attcgtcatg ggaaataaga atgatgtgat ggatgcccgg gctatctgga tggctgttca
     1381 gcagccaggt aaggacatcg cggttaaaac agaagaacaa cagtcagtgc tggttttgca
     1441 ccgttcccgc agacaattgg ttaagttccg caccatgcag attaattctc tgcacggcac
     1501 gctcctagaa tttggtaaaa ccattcacaa aggtcgggca gcgatggagc gagagctccc
     1561 tgaagcactg gagcggatga aagagagact gccgccgtat ctcattacag tgctggaaaa
     1621 ccagtacaac cggctgaatg agctggactc gctgatagag gatattgaaa aaaagcttac
     1681 cagcgtggcg agacagaatg aaacctgtaa gcggttgctg gatattcccg gcgttgggcc
     1741 acttattgca acggcagcgg tggccaccat gggggaagca tcagcgttta aatcagggcg
     1801 agagttcgcg gcttatgttg gtctggtgcc caaacagact ggctcaggag ggaaaatccg
     1861 cctgctgggg ataagcaaac gtggtaatac atatctgcga gccttgttta ttcatagcgc
     1921 aagggcggca gcattactgc ccaaagaacc tggtccgtgg ataaccgaac tgaaaaaacg
     1981 tcgtccaacc agcgtggcaa tcgtcgccat ggcaaacaaa ctggcacgaa cagtatgggc
     2041 gatagcagct catggaagta aatacgacaa gaatcatgtc agtatcagat catattaatc
     2101 tctgataccg ataaatatta acttttaatg aaaggatgaa tactgaaagg taactctggc
     2161 ggccatagtg atgacaacga caggtaagac cgggactcac taaacctgaa tattgtgttg
     2221 agctaaaagc tcgctggaga aatgaggtgt gagtcagcga attacatagg ggctcgcagc
     2281 ttcatggctg caaaaaagcc ggatataaag ctgcaaccta cccgtcactg tcaaggcaat
     2341 ggatgccttg caacgggatg tgttcatata atcaatatgt cagatctatg ctgatacagg
     2401 gtgccagagc ggtattattc agagcctcag agaaaactga ttcacttcgt gtgtgggtta
     2461 attgtattcg ggaaaagcgt ggcttcaacc gggcagttgt tgctcttgca aataaactcc
     2521 ttcggatcgc ctgggcaatt atcgcccgca atgagatgta ttttcccgca gcaaaacaaa
     2581 atcaataatc tacgtaaaat cagtaggagt aaagcttacc ctgaaattgc aaagaatgca
     2641 gaatgatgaa aaacaggtca gaccgacgtt acgaaaacct gcaagatgag ccaggccagt
     2701 aaggccgagt aagcgataag gtcggagcgc gcgaatgtct catcaaggcc agaagcaata
     2761 acttcatgaa caaaccggat atacgaaagt aaacctgtcc ctatcatagt tgctgtactc
     2821 ttgcaatacg ggggaagatc atctcatcac ttacgaacga ttcgtgaaaa cgatgaagaa
     2881 agactacatc gcgtgcatgc cgaaatctaa tgtaagaacg gcattgcata atcttgcagt
     2941 ggagatcggg tattacaatg aaaaccatcc gcatagtgcg ttaggttatc tctcaccaag
     3001 ggaatatcga cgtcagcaag taacgtcacc ttagatgcag tagttgtcag gaaatagtga
     3061 ctcaagggca ggcttaccat atacactctt agtggttcga ctgggtatta gaatagctat
     3121 taacagatta gcactgaaca ctgcgtaata gtggttgcac aaacaaaatg ttatgttata
     3181 acattgctgt attgacatat attttcgaat gtatatgaac gtaatcagaa ctgtcatttg
     3241 tacattaatc atacttccgg tggggctaca gacagcgaca agtcattctt ctatggctaa
     3301 agatacaatc accgttgtcg cgacaggaaa tcagaacacg gtatttgaaa cgccgtcgat
     3361 ggtcagtgtc atcacgaatg acacgccatg gagtcagaat gcggtcacat cagccggtat
     3421 gctgaaaggt gttgcgggtc tcagccagac tggtgcagga cggaccaatg ggcagacctt
     3481 taatctacgc ggctatgaca aaagcggggt acttgttctt gttgacggcg tccgtcaact
     3541 cagtgacatg gcaaaaagca gtggcactta tctcgagccg gcactcatca aacgcatcga
     3601 agttgtccgc ggaccaaact ccagtctgta cggcagtggc gggctggggg gggtggtgga
     3661 cttcagaact gccgatgctg cagattttct tccccccgga gagacaaacg gtgtaagtct
     3721 gtggggaaat gtcgccagtg gtgaccacag cacaggctcg gggctcacct ggttcggtaa
     3781 aaccggaaaa acagatgcgc ttctttctgt cattatgcgt aaaagaggaa atatctatca
     3841 gagtgatggt gagcgtgcac ctaacaagga aaaacctgca gccctgtttg cgaaaggctc
     3901 tgtcagtata acagacagta acaaagcagg tgccagcttg cgtctctacc agaataacac
     3961 cactgaaccg ggcaatccca ctctgacaca tggtgacaac ggcctgcgtg acagaaaaac
     4021 agcacaaaat gacgtgcagt tctggtacca gtacgcccct gcggataaca gcctcatcaa
     4081 tgtaaagtca acgttatatc tcagtgatat cactatcaag acaaacggtc acaacaaaac
     4141 agcagaatgg cgaaacaaca gaacctccgg tgttaatgtt gtcaacagga gccatactct
     4201 gatttttccg ggagcccatc agttaagtta tggcgctgaa tattaccgtc agcagcagaa
     4261 gccggaaggc tctgccacac tatatccgga aggaaacatt gactttacat cgttgtattt
     4321 ccaggatgaa atgacaatga aaagcaaccc ggttaacatt atcgtcggtt cccgttatga
     4381 ccggtacaag agcttcaatc cccgagccgg agaactgaaa gccgaacgcc tgtcccccag
     4441 ggcggcgatt tcagtctcac cgacagactg gctgatgatg tacggctcca tatcctctgc
     4501 attccgtgcc cccacaatgg cagaaatgta cagggatgat gtacattttt accgcaaggg
     4561 taaacccaat tactgggtac ctaaccttaa tctgaaacca gaaaataaca tcacccgtga
     4621 gattggcgca ggtattcaac tggacggcct gcttacagac aatgatcggc tgcagttgaa
     4681 aggcggatat ttcggaacgg atgccagaaa ctatattgcc acgcgcgtgg atatgaaacg
     4741 gatgcgttct tattcttata atgtatcccg ggcccgtatc tggggatggg atatgcaggg
     4801 taattaccag tctgattatt ttgactggat gctttcttat aaccgcacgg aaagtatgga
     4861 tgccagtagc agggaatggc tgggctccgg caatcctgac acactcatca gtgacatcag
     4921 tatacctgtt ggtcatggag gagtttctgc cggatggcgc gctgaacttt cggcagcagc
     4981 cacgcatgtg aaaaaaggcg atccccatca ggctggctat gccgtacatt ccttttcact
     5041 gtcttataag cctgtaagtg ttaaaggctt tgaggcatca gtgactctgg ataatgcctt
     5101 caacaagctt gccatgaatg gcaaaggtgt gccgctttca ggcagaactg tcagtcttta
     5161 tacccgttat cagtggtaac ctcgcaggaa ataaatcatg atgaaaaata caggctatat
     5221 cttagctctt tgtctgacag catcggagca tgccctggcc cacgatgtct ggattaccgg
     5281 taaacaggca gagaacaacg ttaccgcaga gattggttat ggtcataatt tcccctcaaa
     5341 gggcacaatt cctgacagaa ggaatttctt tgaaaatccc cggctttata acgggaaaga
     5401 gataataacg ctgaagccaa cgtccacgga ttatgtctat aaaactgagt ctgcaggcaa
     5461 cggtaatagc tacgttctgt caacgaaaat gaaaccggga tactggtcga gaacctcgtc
     5521 aggatggaag cctgtcagtc gggagggcag aaatgatgtg gcatactgtg agtttgtgac
     5581 taaatatgca aaatctttta ttcctggtga acagcagatg ccagcacaac tctatcagtc
     5641 tccaacaggg gatgagcttg aaatcattcc attatccgat atcagtcgtt tcggtgaaga
     5701 tgtgaagctg aaagttctgt ataaaacatc cccgcttgcc ggagctactc tggagcttga
     5761 ctctgtcagt tatctgaaat catcccgtca tacccatgca gctgagcata aacattcggc
     5821 tcataaagca gaacttacct ttgtaagtaa tgaggacggt atcatcactg taccttccct
     5881 tcatgtcggg caatggctgg ctaaagtcaa aaataagaaa gtatttccgg acaagagtct
     5941 gtgtgatgaa actgtcgatg tggcaacctt aagcttctcc cgaaattaat aatactattc
     6001 ctgccctgat ttatgggcag ggtattatca atacgagaat agcctaccga catccgtgta
     6061 aaaaaccaca taatgatttt tcatatccat ttccagatat acttatgtaa agatcacctc
     6121 caaaaaagcg taatagtaat aaaaatccct gaagcaccct gttcaa
//
LOCUS       DAZGBC010000228         6153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGBC010000228 DAZGBC010000000
VERSION     DAZGBC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.229"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            693..4505
                     /locus_tag="ACHK1J_004642"
     CDS             693..4505
                     /locus_tag="ACHK1J_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO7913809.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNSATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINAFGNIENRDGNSFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRFIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNTL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGLLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4594..>6153
                     /locus_tag="ACHK1J_004643"
     CDS             4594..>6153
                     /locus_tag="ACHK1J_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO7913810.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTAVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGTSEYLSAKATRTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQLKNGYYNTAFFAQYAPRELTLQDQITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGAAVGIDGNIKSL
                     EYNFSVSTPLKY"
BASE COUNT         1761 a         1279 c         1545 g         1568 t
ORIGIN      
        1 ggtaattgct attagatggg aatgaaacct tgtgaaccat tttgctcttc tttctacctt
       61 tcaacggtaa gccaaacata atgtggctgc ttaagctaat tgtcgcaaag ccaaagagct
      121 ctctctatat agctaacctc acggttaatc cgatctcagt attaccatga gcaagaacta
      181 ttgcctttta tataactctt aagggttaca agagtgaatg taaatggtaa tataatgaaa
      241 tggtacctgc ccgtaatgac cataaagaat ctatcccggc gtaaaagata tagagaataa
      301 gatgcagtaa tgctgatgtt ttgttaatca aataataaga tttaaattaa tcgtaactta
      361 tcaaccttgt tgttttattc gaatatctga aatgattctg tacagaaaac tacatatcat
      421 ttcagatatt cgctgaattg gattttttct cacccaggga gaaggggtgg ggacttttaa
      481 attattgtgt attagtaagt taggtacgtc tccgttcgaa gaaaaaacaa taactcttag
      541 ttgttttttg agtccttatt tcagcttatt tttcgtgatt ttttattgcg ccaggtgata
      601 ggatttgccg caatcatttt tgttgatatt ccgcaaatca tcaaaagtga tctgtatgtc
      661 caaaatgaat agcatcaata aggaaaacgt tagtggcgat gataaattta agtaaggaag
      721 caacggtggg taaagcatta acccctattg ctatacttat gatgttgtct tttcctgtag
      781 cttctcaagc ggcgggatta gtcataaaaa atggaacggt atataacgcc aatggtgtgc
      841 cagtcgttga catcaacaaa cctaacggta gcggtttatc tcataatatc tgggataacc
      901 taaacgttga taaaaatggt gtcgttttca ataacagcgc taatgaatcc agtacttcac
      961 ttgccggaaa tattcaggga aacagtaatc tgacctccgg gtcggcgaag gtgatcctga
     1021 atgaggttac ttccaaaaat ccttcaacca ttaatgggat gatggaagtt gcaggggata
     1081 aagcggatct gattattgcc aacccgaatg gtattactgt aaacggtggc ggttcaatca
     1141 atacaggtaa actgacctta accaccggga cgccggatat ccaggatgac aagctggccg
     1201 gttactccgt gaacggcggt accattacgc tcggtaaact ggataacgcc agcccgacag
     1261 aaattctgtc ccgtaacgtg gtggttaacg gcaaagtgtc tgccgatgag ctgaacgttg
     1321 ttgctggcaa taactatgtt aatgccgcag gccaggtgac cggtagcgta tccgccacgg
     1381 gttcccgtaa cggttacagc gtagatgttg ccaaactggg cggaatgtat gcgaacaaaa
     1441 tcagtctggt cagcaccgag aaaggtgttg gggttcgcaa cctcggcgtt attgctgggg
     1501 gggttaatgg tgtcagcatc gattccaaag gtaacctgtt aaacagtaac gcccagattc
     1561 agtctgcaag cacgatcaac ctgacaacaa atggtactct ggataacacc accggtacgg
     1621 tgacatctgt aggcactatc tcgctcaata ccaacaagaa tactatcgtg aatacccgtg
     1681 cgggtaacat ctctacgatg ggtgatatct acgttaacag tggcacgatt gataacacca
     1741 acggcaagct cgccgcagca ggtatgctgg cggtcgatac caatagcgcc acgctgatta
     1801 actcaggtaa agggagttct gtcgggattg aagcggggct cgtggcactg aaaaccggaa
     1861 cgctcaacaa cagcaatggt cagattcgcg gtggctatgt gggtcttgaa tccgctgcgc
     1921 tgaataacaa caacggtgat atccagacca ccggcgatat cgccattatc agtaacggta
     1981 atgtggataa caacaaaggt ctgatccgtt cgtccaccgg gcatatcgtt attggcgcgg
     2041 caggtagcgt aaataatggt tcaaccaaaa ccgccgatac cggcagttct gactctctgg
     2101 gcattattgc agataccggc gtagaaattg gtgcgaacaa catcaataac aacggcggac
     2161 agattgcgtc taatggcaac gtctccctgt caagttacag cacgatcgac gactatgcgg
     2221 gcaaaattct gtccaacagc aaagtgatta tcaagggaag ctcactacgt aacgataccg
     2281 gggggatcag cggtaagcag ggcattgaag tcgccgttgg cggcagcctg accaataaca
     2341 ttggcgtgat cagctctgaa gagggtgata tctccctgtt agccaactca gtggacaacc
     2401 acggcggctt catgatgggg cagaacatca cgatggagtc gatgtctggc gtcaataaca
     2461 acaccgcgct gatcgtggcc agcaaaaaac tgaagataaa cgcgttcggt aatattgaaa
     2521 accgcgatgg caatagcttc ggtaatgcat atggtctgta cttcggcatg cctcagcaaa
     2581 cgggtggaat ggtcggcaag gaaggcatcg agctttccgg gcagaatatc tataacaaca
     2641 acagtcgttt catcgctgag gatggtcctc tgactctgca ggcacagaat acgttcgaca
     2701 acacgcgtgc tctggtcacc agcggggcgg atgcatctat tcaggttggc ggaacgtatt
     2761 acaacaacta cgctaccacc tggagcgcgg gtaacctgga tatcgacgcg accacactgc
     2821 aaaacagcag tagcggtacg atgatcgata acaatgcgac cgggttcata gcatctgata
     2881 aaaacctgtc actggaagtg gtgaataccc ttaccaacta tggctggatc agcggtaaag
     2941 gtgatgttga tgtcacggtg aataacggca acctgtacaa ccgcaatact attgcggctg
     3001 aaaaggggct ggacattgca gcgctgaacg gtattgaaaa ctggaaggat atttctgctg
     3061 gcggcgacct gacgatgaac accaatcgcc atgtgaccaa caactccaac agcaatatgg
     3121 tggggcagaa tatcgttatt aacgcggtta acgatatcaa caaccgtggc aacattgtca
     3181 gtgacgctga cttgaacgtg acgaccaaag gcaacctgta taactatctc tatatggtag
     3241 ggtatgggga tatcgcattg tcggcaaata gcgtggcgaa caataacgcg accatcgaag
     3301 cgacaggcga tctgattatc gattcgaagg gtaacgtggg taacaaccgc ggtaatctgc
     3361 atgcgttgaa cggcgtgttg tctgttaaag gcaacaatct gaacaacgat aacggtgaaa
     3421 ttcgtggtta tggcgatgtc acgctggcac tgacgggcaa ctacgacagc tataagggtt
     3481 tgctgacctc tgaaacgggt gacgtgactc tgacggcgaa catcgtagac aacgcctatg
     3541 gtttgattgc cggtgagaat gtttctgtcg atgctaaatc gacgatttac aacaacactg
     3601 cgctgatcgc ggcgaataaa aagctggtta ttaacgctgg cggcaacctc gaaaaccgcg
     3661 acgggaataa cttcctgcgt aataacggcg cgctgtttgg gatcaccgac aacgttggcg
     3721 gcatcgtagg taaagaaggt gtcacgcttt ctgctcagaa cgtctacaac aataacagca
     3781 gcatcatcgc tgaaaatggt ccgcttaatc tgctgtccag gggaacgctg gataataccc
     3841 gcgcgcttct tagcagtggg gctgatgcca tcatccgtgc ggcagggacg ttctacaaca
     3901 actatgccac cacgtacagc gccggtaatc tcgacgttta tgcggcgtcg ttgaacaacg
     3961 ccagcgatgg tcgcctggaa gacaataccg ccacgggcgt gattgcgtct gacaaaaacc
     4021 tggatctgag cgttgataac agcgtcacta actatggttg gatcagcggt aaaggagatg
     4081 tgcatttcaa tgttctgaaa ggcacgctgt ataaccgtaa tgccatcgcg gcggacaacg
     4141 cgctgaccat taatgccctg aacggtgttg agaactttaa agacattgtg gcgggtactg
     4201 cgctgactat tgatacgcag aagtatgtta ccaacaacag caacagtaat atgttgggac
     4261 aaaccatcgc gatcaatgcc gtgaatgaca ttaataaccg cggaaatatt gtgggtgatt
     4321 attctctggg tgttaaaacc accggtaata tttataacta cctcaatatg ctgagttatg
     4381 gtgtcgctgg cgtatcggca aataaggtta cgaatagcgg taaagacgcc gttctcggtg
     4441 gcttctacgg tttagcgtta gaagcaaacg aaactgataa caccggtact attgtcggca
     4501 tgtaagatgt aaccaaagac agcagtggct tttaatatta atgcgtcatg tcacgcaggg
     4561 gttgctgctg tctttattta gtttgagaag ataatgcaat tacgatttaa ttttggtttg
     4621 ctgctcattc tttgttgttt tcaggttgca gcgaaagaca gtcttgataa tttgatcaag
     4681 cagcaaaaaa atgcagatga aagcacgttg cgcgcgcata aattagaaaa aaaagatgtt
     4741 tactcaaatg cacaacgtaa tgcgtttact ctgggtgatc tgccgcagga aaagaattgc
     4801 tttatcatca atcggattga actggaagat aactttctgg gtggtcagtt gatttctgaa
     4861 gtgcaaaaag cgattgccgg acgttgtgtc ggtagtgctg gtgtgcaaaa aattgctgct
     4921 gccttgcagg attattatat taatgccgga tatatcacaa cgcgaattag tatcccttcg
     4981 caggatattt ctaaaggcaa gctacgtttc caggttaatg ccgggaagat agaaaaaacg
     5041 attgtcgaag gtaatgatat cagtgaatgg atgttacctt ttcagcgtaa cgatattctc
     5101 aatattcgtg atattgaaca ggggctggaa aatcttcagc gagtacctga cgttgatgtg
     5161 aaaattaata ttgaaccggg aacacgggat ggttacagca tcgttcatat tgatacacac
     5221 cgggggaaaa actggagcgt tcgggcaagt tacaacaatt ggggcgatga agaaacgggc
     5281 cgttaccaga caaccgcggt gggttatttg tttaatccag cgaaaatggg ggatctgttt
     5341 tatctcgccg gtacgcgtag tactaccgga cagtatgaaa acgtcagtag ttattattcc
     5401 attccggtgg ggtactggga gtacggcttt ttctatagca aaagtaaatc gcagcaggtg
     5461 attccgcttt cgtacatctc gctggattat gttggcacca gcgaatactt gagcgcaaaa
     5521 gcgacgcgta ccgtttatcg cgataaaaca aaaaaattcg ccggtagtgc agaacttatt
     5581 cgtcgaaagt cgaattacac gcttaatggt gaggagctga cattacaggc gcgggatatg
     5641 ggaaatatca agctggggct aaattataag caacagttac ccggcgcgtt ctgggattca
     5701 accctctcgt ggcaacgctt tttaacctgg ttcggtggtg aaaaaacgcc ggatatgcaa
     5761 tatggcgacg ttagcccggt gagcaatatc tttaattttg agggtaacta tacccgacaa
     5821 ctaaagaatg ggtattacaa tactgctttt tttgcccagt acgcaccgcg tgaactgact
     5881 ttgcaagatc aaattacagt tggcgatcgt tggagtattc gcgggtttga aaatagcgta
     5941 ggtttgtcag ggaatgatgg tttttatata aaaaatacgc tggcatttcc tttgcctgga
     6001 atgaaagcta actattatgc cggtcttgat tttgggcaag tgtatcagga cgcttcttat
     6061 ggcgatgaat cactggtggg cgcggcagtt ggtattgatg gaaatattaa aagtcttgag
     6121 tataactttt ccgtttctac cccattaaag tat
//
LOCUS       DAZGBC010000229         6136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGBC010000229 DAZGBC010000000
VERSION     DAZGBC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.230"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            275..1933
                     /locus_tag="ACHK1J_004644"
     CDS             275..1933
                     /locus_tag="ACHK1J_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO7913811.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CADGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1930..2886)
                     /locus_tag="ACHK1J_004645"
     CDS             complement(1930..2886)
                     /locus_tag="ACHK1J_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO7913812.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3037..4152
                     /gene="macA"
                     /locus_tag="ACHK1J_004646"
     CDS             3037..4152
                     /gene="macA"
                     /locus_tag="ACHK1J_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO7913813.1"
                     /translation="MKKRKTVKKRYVIALVIAIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4149..6095
                     /gene="macB"
                     /locus_tag="ACHK1J_004647"
     CDS             4149..6095
                     /gene="macB"
                     /locus_tag="ACHK1J_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO7913814.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTVPVVNMASGWRQFASGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1426 a         1473 c         1708 g         1529 t
ORIGIN      
        1 caacagcggg ttgccccata tagtcatttg tctgattgac agtgtagtgc aagcaaaaga
       61 tttaatcctt taggcgtaat aaaaaataat ttatcatgct aattatttga ttttgttgtt
      121 tttgcagact tatcagcaag agggagtata acgcgattat tcgctcattt ttcagacatt
      181 tgccatgctt aaatgtgatg tcatcacgta ttagcaaggc ctttcccgtt atactgccag
      241 cgtaaaggat aagtcacata tttctggagg ggatatgatt cttgagcgcg ttgaaattgt
      301 gggttttcgc ggtatcaacc gtttgtcgtt gatgctggaa caaaacaacg tcctgattgg
      361 ggagaacgcg tggggtaaat ccagcttgct ggacgcctta accctgctgc tatcgccaga
      421 atcagatctc taccattttg agcgcgacga tttctggttc ccgccgggag atatcaacgg
      481 gcgagaacat catctgcata ttattttgac cttccgcgaa tcgctgccag gccgacatcg
      541 ggttcgccgt tatcggccgc tggaagcgtg ctggacgcca tgcgccgatg gctatcaccg
      601 tattttttat cgtctggaag gggagagtgc ggaagacggc agcgtgatga cactgcgcag
      661 ttttctcgat aaagacggac atccgattga tgttgaggat attaacgatc aggcacgcca
      721 tctggtgcgt ttaatgccgg tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg
      781 taacggcacg gtgccaaatg tccccaatgt ggaagtcacc gcgcgccagc tcgatttcct
      841 cgcccgtgag ttgtcctcac atccgcaaaa tctctctgat ggacagattc gtcagggact
      901 ttccgcaatg gtgcagctgc ttgagcatta tttctctgag cagggggctg gacaggcgcg
      961 atatcgttta atgcggcggc gggccagcaa tgagcaacga agctggcgct atctggacat
     1021 catcaaccgg atgattgacc gacctggagg gcgctcgtat cgggttattt tgctcggcct
     1081 gtttgctact ttattgcagg caaaaggcac attgcgactg gataaagacg cccgtccatt
     1141 gttgctgatc gaagatccag aaacgcgttt acaccccatt atgctttcag ttgcctggca
     1201 tctgttgaat cttttgcctt tacaacgtat tgctaccacc aactctggcg agctgctttc
     1261 gttaactcca gtggaacatg tttgccgcct ggtgcgtgaa tcttctcgtg ttgccgcctg
     1321 gcgtctgggg ccaagtggct tgagcaccga agacagccgc cgcatctctt ttcacattcg
     1381 ttttaatcgt ccgtcatcgc tgtttgctcg ctgctggttg ctggtggaag gggaaacgga
     1441 aacctgggtt atcaatgaac tggcgcgtca gtgcggacat cattttgatg ccgaagggat
     1501 caaggtcatt gagtttgccc agtccgggct aaagccactg gttaaatttg cccgccgaat
     1561 ggggattgaa tggcatgtgc tggtcgatgg cgatgaagca gggaagaaat atgccgctac
     1621 ggtacgcagc ctgttgaata atgatcggga agccgaacga gaacatttaa cggcgttacc
     1681 ggcgctggat atggaacatt ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt
     1741 ggcgcaaatc ccggaaaatg taccgatgaa tctgcgcaaa attatctcga aagcgatcca
     1801 tcgctcttcc aaacccgatc ttgccattga agtggcaatg gaggccggac gtcgtggtgt
     1861 ggactccgta ccgacgctgc tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg
     1921 tcgcgcggat taaccgcgaa acatcgtggc catttgtggc tgaatagcgt cgagcatctc
     1981 atagcgccga cggtattcag cccgtttttt actggcgatt tcggcaatct cttttcgtgc
     2041 tatctgtgct ggaaggcggt aatggcgttc agcatcacat acgccgccaa ccgattccca
     2101 gaaagcgttg taatcggcat ggatcttgcc ttctttatcg cgataacgca ggctgcggta
     2161 aatatgcgtt tcattgctga cggcaataat ctgctctacc tgcaaacgtt gggcaaacag
     2221 acaggccgct tccatcacga ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt
     2281 ctggatttcc tgatgtggga tttcccattt tgcgccttgc agtccgccaa taaacatcgt
     2341 tcttttcccc tgatattcac acagggtaaa cgtgatctct gccagaggaa taccttcgct
     2401 gttgcggaac aggattgtgc tgtcaccttc tttatccatt gagatcatca tggtcagctc
     2461 aagcgtgaac tgctcgccgt ttttgccttc cagcttcgcc agttgcagcc cgggggtatt
     2521 caaatataag ctgaattctt ccgccgacat acacccgcgg agtaacgcat aatggtaacg
     2581 taacgcctcc agcaattgct tacggctaag attcgccgca aggtaagggc gatgcagacg
     2641 tacaggcagt cgcggctggc gcgttaacaa tacattgaga ttaggccagt gggaaagttc
     2701 gttcatccac tcaacgctta aacgcggcat aatcaacgag cgcagcaaaa atttctggcg
     2761 aaaactacgg cgatgccaga atttacccgg ccgacactgt ccacgtgcca gactaagaaa
     2821 aagtgacagg ctgctgagag attcagatgg cgtaaaggtc cgttcagtta gctgcgacat
     2881 attcatgaaa tcaatggtta tacatgacgt cgatttcacc attgcgtatc ttaaccaaac
     2941 atcaatagtg tgattactaa cgtaaatttt agggttttgt tgatatttcg ttgaagttaa
     3001 tgacccggat tggcatatgg agtattcaga atatttatga aaaagcggaa aaccgtgaag
     3061 aagcgttacg ttattgcgct ggtgatagcc atcgccggac tgattacgtt atggagaatt
     3121 cttaacgcac ccgtgccgac ttatcagaca ctgattgtgc gccccggtga tttacagcaa
     3181 agcgtgctgg cgaccggaaa gctggacgcg ctgcgtaagg ttgatgtggg cgcgcaggtc
     3241 agcggtcagt tgaaaacact gtcggtggcg attggcgata aagtaaaaaa agaccagctt
     3301 ttaggggtca ttgatcctga acaggctgaa aaccagatca aggaggtcga agcaacgctg
     3361 atggagctac gcgcgcagcg gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg
     3421 tattcccgtc agcaacgtct ggcacaaacg caggcggttt cacagcagga tctcgacacc
     3481 gccgcgacgg agatggctgt gaaacaggcg caaattggca ccattgacgc gcaaatcaag
     3541 cgcaatcagg cttctctcga tacggctaaa accaatctcg attacactcg catcgttgcc
     3601 ccgatggccg gggaagtcac gcaaatcacc actctgcaag gccagacggt gattgccgca
     3661 caacaagcac cgaacattct gacgctggca gatatgagca ccatgctggt aaaagcgcag
     3721 gtttctgaag cggatgtaat ccacctgaag ccggggcaaa aagcctggtt tacggtactt
     3781 ggcgatccac tgacgcgcta cgaggggcaa atcaaggatg tactaccgac gccggaaaag
     3841 gttaacgacg ctattttcta ttacgcccgt tttgaagtcc ccaaccccaa tggtttactg
     3901 cggctggata tgactgcgca agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg
     3961 atccctctgt cggcgttagg cgatccggtt ggcgataatc gttataaagt caaattgttg
     4021 cgtaatggtg aaacacgcga gcgtgaagtg acgattggcg cacgtaacga taccgatgtt
     4081 gagattgtca aagggctgga agcgggcgat gaagtggtga ttggtgaggc caaaccagga
     4141 gctgcacaat gacgcctttg ctcgaattaa aggatattcg tcgcagctat cctgccggtg
     4201 atgagcaggt tgaggtgttg aagggcatca ccctcgatat ttatgctggt gagatggtgg
     4261 cgattgttgg cgcttcgggt tccggtaaat cgaccctgat gaatattctc ggctgtctgg
     4321 ataaggccac cagcggcacc tatcgcgtcg ccggtcagga tgttgccacg ctggacgccg
     4381 atgcgctggc gcaactgcgc cgcgagcatt tcggctttat tttccagcgt taccatttgc
     4441 tttcgcattt aaccgccgag cagaacgttg aagtacccgc cgtctatgct ggtcttgagc
     4501 ggaaacagcg gttgcttcgc gcccaggagt tgctgcaacg actggggctg gaagaccgta
     4561 cagagtatta tccggcacag ctttcgggtg gtcagcaaca gcgcgtcagc atcgcgcggg
     4621 cattgatgaa cggcggtcag gtaattcttg ccgatgaacc gaccggcgca ctggacagcc
     4681 attctggcga agaggtgatg gcgatcctgc atcagctgcg cgaccgcggg catacagtga
     4741 ttatcgtcac ccacgatccg caggtcgctg ctcaggccga gcgggtgatc gaaattcgcg
     4801 acggcgaaat tgtgcgcaat cctcccgcca ttgaaaaagt gaatgttgct ggcgggacgg
     4861 tacctgttgt caacatggcg tctggctggc gtcagtttgc cagcggtttt aacgaggcgc
     4921 tgacgatggc atggcgggcg ctggcagcga ataaaatgcg tactttactg accatgctgg
     4981 ggattattat cggtattgcg tcggtggttt ccattgtcgt agtaggtgac gccgccaaac
     5041 aaatggtgct ggcggatatt cgttctattg gtacgaatac tattgacgtc taccccggga
     5101 aagattttgg cgatgacgat ccgcaatatc agcaggcgct gaagtacgac gacttaatcg
     5161 ccatccaaaa acaaccgtgg gtcgcttcag ccacacctgc cgtctcgcaa aacctgcgcc
     5221 tgcgttacaa taatgttgat gttgctgcca gtgccaatgg cgtgagcggc gattatttta
     5281 atgtctatgg catgaccttc agtgaaggaa acacctttaa ccaggaacag ctgaacggtc
     5341 gtgcgcaggt cgtggttctc gacagtaata ctcgccgcca gcttttcccc cataaagcag
     5401 atgtggttgg cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg
     5461 aagaaaaaca gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttacagca
     5521 cgatgtccgg gcgagttatg ggccagtcgt ggctgaactc cattactgtc agggtgaaag
     5581 aaggatttga cagcgccgag gcggaacagc aactcacgcg tttactttca ctgcgccacg
     5641 gtaagaagga tttctttacc tggaacatgg acggcgtctt gaaaaccgtt gaaaagacca
     5701 cacgtacttt acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta
     5761 ttggtgtaat gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc
     5821 gcatggctgt aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac
     5881 tggtttgcct ggtcggtggc gcgttgggaa taacattgtc actgttaatt gctttcacct
     5941 tgcagctttt cttacccggt tgggagattg gtttttcacc gttggcactg ctgctggcgt
     6001 ttctctgctc gacggccacc gggattttat ttggctggtt acccgcacga aatgcggcac
     6061 gactggatcc agtagatgct ctggcacgag agtaattttt gagataaaaa tgccagccga
     6121 tcgggctggc attttg
//
LOCUS       DAZGBC010000230         6124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGBC010000230 DAZGBC010000000
VERSION     DAZGBC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.231"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..244
                     /locus_tag="ACHK1J_004648"
     CDS             <1..244
                     /locus_tag="ACHK1J_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913815.1"
                     /translation="CMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGR
                     ISRRRTWHAACRMAEQLTTLRYRFGLQGDGIVAQPC"
     gene            327..806
                     /locus_tag="ACHK1J_004649"
     CDS             327..806
                     /locus_tag="ACHK1J_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913816.1"
                     /translation="MHSLLILLYVVVWRACIILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            1002..1379
                     /locus_tag="ACHK1J_004650"
     CDS             1002..1379
                     /locus_tag="ACHK1J_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913817.1"
                     /translation="MYSPLIAHYSALQTQSALLADISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            1461..2090
                     /locus_tag="ACHK1J_004651"
     CDS             1461..2090
                     /locus_tag="ACHK1J_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913818.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            2206..2589
                     /locus_tag="ACHK1J_004652"
     CDS             2206..2589
                     /locus_tag="ACHK1J_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913819.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            2682..3659
                     /locus_tag="ACHK1J_004653"
     CDS             2682..3659
                     /locus_tag="ACHK1J_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO7913820.1"
                     /translation="MKTKKHTSRTATKTPEHDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMETVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSSDALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRKSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            3769..4203
                     /locus_tag="ACHK1J_004654"
     CDS             3769..4203
                     /locus_tag="ACHK1J_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO7913821.1"
                     /translation="MNDNALVDVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKEGLQSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            4207..4521
                     /locus_tag="ACHK1J_004655"
     CDS             4207..4521
                     /locus_tag="ACHK1J_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913822.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKAELWYYNLFINPSADELMDVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            4605..5552
                     /locus_tag="ACHK1J_004656"
     CDS             4605..5552
                     /locus_tag="ACHK1J_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7913823.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRQGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            5664..>6124
                     /locus_tag="ACHK1J_004657"
     CDS             5664..>6124
                     /locus_tag="ACHK1J_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO7913824.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQMGNVKALFENKPFITLSEPACGAGSMILAMADTLNR"
BASE COUNT         1367 a         1406 c         1812 g         1539 t
ORIGIN      
        1 ctgtatgttc cgtcgtggcg gtgtctggac accctgcctg tattaccggc agggaatgta
       61 tcgcaacgag tacacgggat ggtatcagcc gttaccgcgt gaggtcattt atctgaaccg
      121 gtttaccgga cggatatccc gtcgcaggac atggcatgct gcctgccgta tggcggagca
      181 actgaccacc ctgcgttacc ggtttggtct gcagggtgat ggaatcgtcg cacagccctg
      241 ctgatttatc atttattacc ctgtcgggaa cacgcttccc gcagggcagg tgtgttcccg
      301 ttattttttg tatctgaggt gaacacatgc atagtctgtt aatactcctt tacgttgttg
      361 tatggcgtgc ctgcatcatt cttcgggtaa tcgcaccagt gtcggctgtc cgtgcgtggt
      421 gtgagtatca actggaaggt atgacgatac ttcgtgatgc gtacagctcg ctggctaagg
      481 cggctggaat gcagaatgat gcatttgaat atactgaaag tattgcacgt catctgggct
      541 acaaaagcgg tggtgaccaa ctgaaatcag gacttttccg gccgtttctg tggcatatac
      601 gtgtgaaagg ccacaggctg ggggctgtca tggagatgga ggcatggaag acatggaaga
      661 atggctgtaa cgatgtctgc aatccatggg aggatgtctg caatccatgg gagcgtggat
      721 ggaacgatgg ctggacggaa tgtctgaatc aacgtatagc cgggctcctg aagcaggcct
      781 gtattgaatt ttccgacgca gactgacggg caggatgcca gtcagtcaca ggaaggatgt
      841 caaatcgtga gcgatgcaac tgccattttg caatgatggc tgttttgctc ccgtaacggt
      901 ttttctttta cttactgcac cgtgtttttg cgccggtgtt ttccctgacg ggagttttgt
      961 tccgtcaggg acaggacttc ctttaagaga cggaggtcat catgtattca cctttaattg
     1021 ctcattattc agcgcttcag acgcagtctg ctctgctggc tgatatcagt cagatggaag
     1081 gcgagctgat gcggttacgt gcctggcaac ggctgggtat taccccggag ccggatgatg
     1141 agacggagcc ttcactggta tacgtgtatc tgtgggatac cggcgaggcg ctgggctggc
     1201 tgttgtatga cctggagcag gaggagattt ccgcaccggg aattcaggcc cgtcaggcac
     1261 tcgacagtct gatggctctg gggcgtgaaa ttaatcatga aatctgggct gacagtatca
     1321 gtaccggaaa gatggtggcc ggagcggatg aatattttgc ccggcacgac atgatgtaat
     1381 tcatgacctg accctgacgg gggagttctc cgtccgggag acattccccg ttgtttcgtt
     1441 ttaaccatga ggaatgttcc atgtctgagt tatcttcacg tccggcccgt gagccggtgg
     1501 tatataccct tgagcaggtt tccacaatac cggaaaagca gtggcatgct tttgttctgg
     1561 ctgtcactga aaccttctgg cagttgccgg aggcgttacg gccgcagaat gcgtatttcg
     1621 gctccctgac ccgggcgtca gagttgtttc cggtaacgga cacgctggcg ttttactgcc
     1681 ggtctgccga cgggctgtgg tcggtgaatg tcactattga gcgggagcac agccggaata
     1741 tcctggctct taatgagctg aatttcggac gtcagccggg agatttcttt gcccgtacag
     1801 tctttgtgct gttgcacaat ctgtgtccgg actgttttcg tattcattcc actgtcggtg
     1861 gtgccagctg gtcgctgccg ctgaaatgga taaaacggta cctgggacat gagaattttt
     1921 ctgctccgga gtctgtatta accaccccgg taaggggaga tgcgtttgac agcttactgc
     1981 tgcagttttt gtccggtcag gggcgacagt tatccccgga tgactgggca gcactggaag
     2041 aggccgaata tcagctttac tggctgcggg ctcttgccgg tgggcggtaa cagaacgccg
     2101 tagtttcttt atcaggaata acgtcatcac gtattccgtc attatatcac ccggcaggga
     2161 catatccctg tggggagagt cccggcttta ttcaggagaa ttgttatggg actcgatatt
     2221 tactttctgg ctcgtgataa ccatcacagt ggtgccgttg tgggaacaca ggaacatcag
     2281 gaggtggggg ctttccgtaa ggtgaaaccg cttctgacat ggtttcgtga acataccggt
     2341 gcttcagatg agcagcatgc tttccctgta acccgcagtt cactggctgc attattgcgc
     2401 gacctcagta tgctgacacc ggggaactgc cacaggctgt ttccttatgt cacaggctcg
     2461 tgtttacggg gggaagatta tgccggtgat tactggtatg acgtggatga cgtaaaagcg
     2521 tgggtcagca atacgctgaa gacgtttgat tttgagcgtc gttcactggt gatgctggcg
     2581 ttgtggtaat tctggccagt ctgattttcg ttattaattt aactgtacgg tgcactctca
     2641 ccgtacaggg agcgtgcatc tttttcaggg ggcatgctct tatgaaaacg aaaaaacata
     2701 cttcacgtac cgcgactaaa actccggaac atgacctgta tcagcttgtg accgacaggg
     2761 ttgttacggc cattgaaaat ggtgtgccac cctggaaacg tccctggcgc tctgcgcgta
     2821 atggcgtggg gcttccggag atgatgccgg caaatgccct gaccgggaag ccttatacag
     2881 gggtgaacgt cctgttgctg tggctggccg cggatgaagc cgggtatgtc agcaatgact
     2941 ggctgacgta ccgtcaggcg caacaactgg gtggccacgt ccgtaaaggg gaggtggcca
     3001 cgctggcggt gatttacaaa gactggaaaa aacaggctgt ggatgaacac ggtaaccggc
     3061 tgacggacag tgagggggag cctctcatgg aaaccgtacc ggtactgaaa tcgaatcctc
     3121 tgtttaatgt ggggcagtgt gacggtttac ctgaacatct gatggtgaag gcggaaggca
     3181 gctcggatgc actgccggca gataccttca gtgagcgggt gctgcaggta ctgaatggca
     3241 gcggcgtcag cgtctcgtca cttccacagg acagggcatt ctaccggcct gatcttgacc
     3301 gtatcgtgat gccactgtcg tcgcagtttg accaggtggc tgattactgg accaccctgc
     3361 tgcatgagct ggtacacagt accggtcatg aaaagcgcct gcggcgggtg gggataagta
     3421 cctttaccgg tcgcaaaagc ccggaatatg cgtttgaaga gctgattgcc gagatgggca
     3481 gtgcttttct gtgtgcacat ctcgggattg ccggtgacgt acagcacgaa agttatattg
     3541 acggatggct taaggttctg aagtctgaca aaaaagcgct gtttcgcgcc tgccggcagg
     3601 cacgtgaagc atcagagtat ttactgacgc ggcgaacttc gcaggagtgc gcagcataat
     3661 ttttactacc ggtggcggtt ataacctgac cgccatttat cccgtgaggg cattgtccct
     3721 cacggggcag tgctctctgt tatttcagga ttacagagga actgagtgat gaacgataac
     3781 gcactggttg atgttgacga tgctgttgtg gcccgtatca gggatgtgct ggcgtataca
     3841 tttccgggac agaaattttc tgtgcggaaa tgcgatccat ctttcccgaa agttttcaac
     3901 aatgtgtggg tgagctggct ggatggtcca acggaggccg aagtacgctc tgtcacagag
     3961 gggtttgaga ataaagaggg gctgcagtct gtgcttgaat cccgttattt cagcgatgaa
     4021 ctggtacagg agtgtattga tcgccttcgt aaagagtacg gacaggggaa cgtcccgttg
     4081 agtgtgaccg tggagcgtta ccggaaaaac gagctggaga agattgaaac agcgcgtttt
     4141 ccgggcaaca tatccgtagc cataaacgag atcgggataa ataccagcaa atacaggagg
     4201 taaggtatgg ctggtaaatg ccttgtctgg ttctgtaacg ggatctctga accgtgtctg
     4261 atagcaggaa atatcagtga attactggca gatgttacga tcacgtcaaa ttaccgtgga
     4321 gatgtcacat ctgaaatcag ggatgttgta caggagtggc tcaggaccgg cagggggaac
     4381 ctggcggatt taaaggcaga gctctggtac tacaaccttt tcatcaatcc gtctgcagac
     4441 gaactgatgg atgtcaacag gcggtatggc cctgagcgaa ctgcctggct gcagggtttt
     4501 atccgcaaca acggggcgtg ataccccgtt ttcccgtgag ggcattgtac ctcacgggac
     4561 aatgccctct ttttacatct gtataaaaaa gaggagcgta aacaatgtct gaatggtgca
     4621 aaaaccgttt tgaaattacc ggtaaatcag tctgtcttga tgtacttaca cagtggattg
     4681 aagggcgtga gaccccgcgc taccgtcatg ccatacagca gagtatacag ctgtttctgg
     4741 ccggttgtgc agggctggta aaacccacac ggacgacgca gtatccgcca tatccggcgc
     4801 tggttcgtca aggaacgggg ttgtcatctc cggctaacca ggcgtttgag cagtggctgg
     4861 ggttgctggt aaaaaatgcc gttctggatg gtgaaacgat aaagaccatt gagcgtcttt
     4921 atcaccagtc atgtatcggc gctgttcgct ggggtaatct gccggataat gccagggaga
     4981 tcatcaccac gctgatgagc cgtcagtatt ctgactggtt tggtctggcc gggctgagtg
     5041 aacacattga tgcagaagcc tgctggagct ggttgtcgga ttacccggaa caggcgcagc
     5101 cctgtgacat gctgatggtc ataccctccc gtctggctac agagctgaat ggttcaggtg
     5161 gactgctgca gggtatttcc acaacgacca gcctttacag ccggatatac ggtgtggagt
     5221 ggccgtcagg aaataatgtt cgccatgcgc gtgatgagat gagcagtctg gtgctgctga
     5281 tcgacacgcc ctggtatccg ccttcaggcg aactggttgg tgagatctcg aaggtgtttg
     5341 actgcgaggt ccgacactgg tacagcgagc cggtacacgg tatacagggt tacaactgtt
     5401 acgatggcgg agagcatacg gacagtgatc tgcaggccga atggccgggc agggaaacac
     5461 ttccacagcc ccgactgtac ctggtggagg agtgcgctga agaacagacg aaagccgcac
     5521 ctttaccggt tcctctggtc tcagcccagt aattaacctc cgggtatcat gtccgtgatg
     5581 cccgttttat caacatttca acccgaaggg gaacacctgt tcccctgtcc gggccatgtg
     5641 ttccctgttt ttacggagaa aacatggcca ccatgactga ccatcagaag gagttcattt
     5701 cgttgtttaa ccggacagcc tgttatcaca aacgtcataa ggtgtttgaa gactttgtca
     5761 gctgtagtgt gattgccctg gaaaaccggc ttcagttcag tgaggcccgg gaacagaagt
     5821 atctgcggat cgtcagtggt tatgaaaagc aggatgttat ccacattgcg catttgcttg
     5881 ctcatgtcat tgagggactg gagcagggat tctgtgattt tctgggcagt gtgttcatgc
     5941 aactggaact gggggataaa taccgtagtc agtttttcac gccatggagt gtggccagca
     6001 tgatggcaca gatgcagatg gggaatgtga aggcgttgtt tgagaacaaa ccctttatca
     6061 cgctcagtga acctgcctgt ggtgccggca gtatgattct ggcaatggct gacaccctga
     6121 accg
//
LOCUS       DAZGBC010000231         6100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGBC010000231 DAZGBC010000000
VERSION     DAZGBC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.232"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2151)
                     /locus_tag="ACHK1J_004658"
     CDS             complement(<1..2151)
                     /locus_tag="ACHK1J_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7913825.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTDCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSTGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVTALTDNTQGA"
     gene            complement(2216..2815)
                     /locus_tag="ACHK1J_004659"
     CDS             complement(2216..2815)
                     /locus_tag="ACHK1J_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7913826.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDILGLVTSFSYAGDKNRQLSRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(2882..>6100)
                     /locus_tag="ACHK1J_004660"
     CDS             complement(2882..>6100)
                     /locus_tag="ACHK1J_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7913827.1"
                     /translation="IAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTI
                     TSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQ
                     YLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVGV
                     QVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKD
                     RHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLI
                     KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDRE
                     ITLPSSGTTLISLVDGEGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQ
                     RLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTA
                     EVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQLA
                     LGNYSLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDK
                     WNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMF
                     VAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLN
                     NVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQVMI
                     PPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAG
                     QGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIN"
BASE COUNT         1163 a         1829 c         1583 g         1525 t
ORIGIN      
        1 tgccccctgc gtattgtctg tcagcgcggt tacccgacct tttgaagcca gattaactga
       61 tgatactgct gttccacctg acggtaacgc cccgatctct gatgccgttg gcttatttct
      121 ggagttatag tcccttcgcc agccaggtga ataatctgtt ccgtgattaa tataggtaaa
      181 ctgggcgtta gttgttccac caccagtgga ggtggtcggt gtggtaatgc ggatcgtcat
      241 cgctgacttt atccccatta cttcaatgac agctccggcg agatgaatat taccgcagtc
      301 agtatcagta atgattttat tattgccata agaccaggaa cccttgcaca tccagtaggg
      361 atggttaaat gctccctgag aatccagcca ctcgataaac tgtgcagtcg tccagtttcc
      421 tgttgttgtg cttactgacc caccgaaggc acggcaggca ccaatatttt tcgtaaaggt
      481 gtctttgcca gggatatccg caccgttctg atctttctgc agacgtttct cagcattgtc
      541 attggctgct tttactgcct ttggcgttgc cgccagcgtt tcagacgtgc tgttggtcgc
      601 gctgcttagc tggattatcc ctttctgtgc tgtcgttgca tcctgtgcgg tgtatttccc
      661 gttagccagg tcatacgcgg ccttaacggc ttttggtgtt gccgccagtg actcggaagt
      721 gctgttagtc gcactactga gctgtactat cccctttttc gtcgtgctcg catcctcaag
      781 cgccacggcg gatgcaatat cctctgcccg ttttgccgct gtctcagcgc gcgttgctgc
      841 agattccgcc gtacttttgc tctgagctgc tgccttcgca ctaccagcag cctctgtcgc
      901 cttcgtggat gccgtcgtgg cgctgccctt cgctgctgac gcctgtctgg tcgcctcatc
      961 ttttgaagca gacgccgatg atgccgatga cgccgccgaa ctggcggacg atgccgctgc
     1021 cgtttttgag gattctgcac gggtttccga cgctttcgcg ttcgtttcgg atgtcttcgc
     1081 tgcggaagct gacctcgctg ctgccgtggc ctgctcagtg gcttcgccag ccttcgttgt
     1141 ggctgttgaa gcggatgatg cggcgctttc tgccgatttt ccggcggcgg tggcactggc
     1201 tgaggcctgc ccggcacttg ttgacgcggc actggcagac gacgcagccg ctgtttttga
     1261 gcctgccgca gctgaggcgc tctgtcccgc tgccgtctca gaagacctgg cgttcgtctc
     1321 ggacgttttt gccgccttcg cggaatttcc tgccgccgtt gccgaggaag ctgcgctact
     1381 ggcgctcaag gatgcgttcg tttctgatga tttcgctgcc tcttttgagg ccgccgcatc
     1441 ccgggctgag gtggcagctt ctgacgcttt cgtggtcgcg gtggatgcag aagtggcggc
     1501 tgattgttct gacgctgcgg cattcgtttc tgacgttttc gccgcaccgg cactggtagc
     1561 tgccacgctt tttgaggact ctgcagcggc agcacttttt gaggcttcag tggcctttgt
     1621 tgatgccgtt cctgcgctgg aagacgctga ctgagccgac gaagcggcct gtccggctga
     1681 cgtgctggct gcacgtgctg aatctgcagc atcagtcgca tgggttgccg cctcacgggc
     1741 agatgtgctg gcatcactgg ctgacttctt cgcggctgcc gtgttctgtg ccaccacgga
     1801 cgcgttacgc gccacctctt ccaccatcag ttcaaaacgg cgcagtgcct caggacgggc
     1861 atcatcctcc gtcatggcac cgagaaaatc attcagcgta ccgggtcggg aatcttcata
     1921 cacggtgatg gtcccggcat gtgacggcgg gaatccttcc accaacagaa taacgctgta
     1981 ctgaccgtac tcaacgtcca tgctgtaacg cccggcttca tccggatttt ctgaggccag
     2041 cgtgttcacc accaccgtgg tgctgttacg ttttgctttc agctggattg tgcagttctg
     2101 taccggtttt cctgtgccgt ctttcagtac acctgaaatc tttactgcca tattcacccc
     2161 acaaaaaagc ccgcctgaac cggcgggctg tcataacact gtgttacctg gctaatcaga
     2221 acttataacc gacacccatg atgaaaccgt cagtgcgcca gtcgccactg ccggagcctt
     2281 cataagcaat atcaatggcc acggattcgg tcgggttaaa ctgcacgcca gctccccacg
     2341 ccagagacgt gttgctgtgg cgaccgtcat cacttccggt cagcacgtcg tgcgttttcc
     2401 ccttgttgtc agttacgcgg agataatccc cggagaaagt cgacacacgg ctgtaagcca
     2461 cacccgccat cgcatacgcg ctgaaccatt cattcacgcg cacagacggc cccaccatca
     2521 cgctgaacca gcggttacga acggaatctt catgccagcg ggtatcgctg taacggctca
     2581 gctggcgatt cttgtctcct gcatagctga atgacgtcac cagccccagt atgtccgtaa
     2641 actcataacg gtatttcacg ttaatcccgt tcagatcatc gctgccggga acgttcgtcg
     2701 aggcatgaag ataccccgcg ctcagcgtgg actgatgttc agacgcccat gcaggcgcac
     2761 cggatacggc cagacaaatg gctgcggaca aaatggcggc ataaagttta cgcataatta
     2821 cctctcgctt ttctgcaata aaaaaggcgt catttctgac gcccgttatg ggttataaaa
     2881 ttcagttgat actgatacct gctgtggatt tcttcatcac cacaaccagc agatcgctga
     2941 tactggttgt tggtgtccag ttattcgctc ctgatgaaga tacggtgaat gtcagtgtca
     3001 gtgtcccctg tccggcaggc atatctataa ctgaggaaaa tacgccctga gcatccgtcg
     3061 tggactgatt aaaaatctcc tgaccattgc gggtcactct taaccggcag gttgaatacc
     3121 agtatgactg ttggttatta ctgttgaaat tctcatgctt accaccgcgg aataacactg
     3181 gcggtatcat gacctgccgg tcaaatttct gatcatcact gattcttacc gtgatggtgc
     3241 cactggcata actgttcgtg cgggggaaag acttgctgac cgttttgaca atatcacctt
     3301 caatctggtt ggctgacagt ttccccttaa tctgacagtt ctcattaatc gtgacgttgt
     3361 tgagcgtccc tgagttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
     3421 ttccgtctga tgtcagggaa aataccggag gactgccacc gctggtaatg gtgggtgccg
     3481 tcaggcgttt caggaacacg tcgttcatga atatctggtt gccctgcgcc acaaacatcg
     3541 gcgtttcatt cccgtttgcc gggtcaataa acgcgatacg gttagcggca accagaaact
     3601 ggctcagctt gccttcctcc gtatcctcca tactgaggcc aatacccgcg acataatgtt
     3661 tgccgtcttt ggtctgctca attttgacag cccacatggc attccattta tcgttggcat
     3721 ccttccactc tttcgaaaac tcatccagtc tgctggcgtt atcctccgtc agatctactt
     3781 tttccagcag ctctttaccg agatgggatt cggttatctt gcctttgaaa aaatccaggt
     3841 aaccttccgc atcatcgctc gcccgaccga cggcctccac aaatgccgat ttgccaacgg
     3901 tgttcacact gcggatataa aagtaataat catggcccgg tttgatattg atactggcgg
     3961 ctatccagta cagccccgtg ccaaggtagc gggctgtggt ttcaacctgc ctgatatcgg
     4021 taatccgcgt ttccgagaac cagaactcaa actgtacagt cggatcataa accgcaagat
     4081 gcggcgtggc ggttatctga aaatagcccg gcgtcagctc aatccgcgat ggcgctgccg
     4141 gtgcggcaat ccggaacgat accgacgccg gatcgccctg ctgcccccag gcatttaccg
     4201 cccggactgt cagactgtaa tttcccagag ccagctgcct gaagcggtat gtggtttccg
     4261 tcgtccgggc cgtgctgacc agccgctcac tgccgtcatc cgctgccacg gtcaggcgaa
     4321 gcataaagct cacgcccttc accaccttcg gcgtatccca gcgcgccagc acctggtatt
     4381 ccccgctgtc tgcggtgact tctgcggtca ggtgttgcac tgctggcgga gtgacaccat
     4441 tcaccgtgcc gctctggtcg ccgtcaaagt gcgccccgtt atccacgatg gcctctttct
     4501 ccggtacatg ctgcacggca gtgatggcat acgtgccgtc gtcgttctca cggatactca
     4561 cgcagcggaa caggcgctgg cgcaacgtcg gcaacttcag cccccacacg ctgtattcag
     4621 caacgccgtc gggaacacgg ctcactttca ccttcacgcc gtcggtgacg gactggacct
     4681 ccacgctgac cggattaccc tcaccgtcaa ccaggcttat cagcgtggtg ccggaggatg
     4741 gcagcgtgat ttcacggtcg agcgtcagcg tccgggtctg gctgtttacc gccagcacgc
     4801 gcccgccggt gctgataccg gcatagtcat catcacagat ttcaatgaca tcgcccggca
     4861 catggcgaag cccttctgcg cccacgctga agtccacggt ctgcgtttcc agcagttctg
     4921 ttttaatcag ccacagcccg gcgcggtgtg cctgcccccg gctggtacag ccaaaagcat
     4981 ccatcttcgt gacgttacga ccgtaacggg caatggcctg cgtatcctcc acaagctctg
     5041 tcgccgtctc ccagccgttg ttcgggtcaa tccagttcac ctcaacggca ttatggcggt
     5101 ctttcagggc gctgaagctg tagcggaacg gcgcgccatc atccggcatc accacattac
     5161 tgcggttata ggtccacacc ttatccgatg gccggtcctg cacaaacgtc agcgtctgcc
     5221 cgttccatac cggcatacag cgcatcgccg agcagaaatc actgagcaca tcccacgcct
     5281 tgcgctgcgt ggtcaggtac gcattacagg taatacgcgg ctccgtgcca ccaaagccat
     5341 ccggcaccga ctggtcgcaa ttctggccga tgacatacag cgcccattta tccacatccg
     5401 ccgcaccaag acgtttcccc atgccgtagc gcggatgggt cagcatatcc cacagacacc
     5461 aggccatgtt gttgctgtat gctggcttaa acgttccgtc ccagataccg ctgtattgcc
     5521 gcgtctgcgg gttatagttc gacggcacct gcagaatgcg tccgcgaaga tgataattac
     5581 ggctcacctg ctggctgccg aactgctccg agtccacctg cacgccgacc agtgccgtgt
     5641 tcgggtagca ctgtttcaca tcgatgattt cggtgtatga cgaccagagc gttttgttct
     5701 gcagctggtc tgtggtactg tccggcgtca tcctgcgcat ccggatatta aacgggcgcg
     5761 gcggcaggtt atccaccacc accgaggcca gatactgcga ggtggttttg cccttaatgg
     5821 tgatgtcttt ttccgtcacc cagccaccat tacgctgtat ctgaaccagc aggcgaactt
     5881 ccgacggatt ccggtccccc tttgaggtgg tttccaccag tgcctgcaca ccgaaggtaa
     5941 agcgcagacg gtcgatgttt gcagacgtga tggtccgggt gatcggcgtg tcgtatttca
     6001 cttccgtacc cagcaccgtc tcggatccgg aggattcaaa tccctccggc ggtgtctgct
     6061 cctgctcacc ggcccggaac accaccgtga caccggcgat
//
LOCUS       DAZGBC010000232         6086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGBC010000232 DAZGBC010000000
VERSION     DAZGBC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.233"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(50..1441)
                     /gene="tcyP"
                     /locus_tag="ACHK1J_004661"
     CDS             complement(50..1441)
                     /gene="tcyP"
                     /locus_tag="ACHK1J_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO7913828.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQILKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1574..2164)
                     /gene="ydjM"
                     /locus_tag="ACHK1J_004662"
     CDS             complement(1574..2164)
                     /gene="ydjM"
                     /locus_tag="ACHK1J_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7913829.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2327..2995)
                     /gene="hxpB"
                     /locus_tag="ACHK1J_004663"
     CDS             complement(2327..2995)
                     /gene="hxpB"
                     /locus_tag="ACHK1J_004663"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO7913830.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3142..3678
                     /gene="yniB"
                     /locus_tag="ACHK1J_004664"
     CDS             3142..3678
                     /gene="yniB"
                     /locus_tag="ACHK1J_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO7913831.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3719..4579)
                     /gene="yniA"
                     /locus_tag="ACHK1J_004665"
     CDS             complement(3719..4579)
                     /gene="yniA"
                     /locus_tag="ACHK1J_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO7913832.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4685..4975)
                     /gene="ghoS"
                     /locus_tag="ACHK1J_004666"
     CDS             complement(4685..4975)
                     /gene="ghoS"
                     /locus_tag="ACHK1J_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7913833.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5076..6005)
                     /gene="pfkB"
                     /locus_tag="ACHK1J_004667"
     CDS             complement(5076..6005)
                     /gene="pfkB"
                     /locus_tag="ACHK1J_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO7913834.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1556 a         1542 c         1509 g         1479 t
ORIGIN      
        1 aagtaaggca acggccccta tacaaaacgg accgttgcca gcataagaat taatggtgtg
       61 ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc agccactggc
      121 tggtcagcgt gccagctgtc atcgagccac taacgtttag cgccgtacgg cccatgtcga
      181 taagcggttc aacggagatt aacagcgcca ccagggttac tggcaggccc atcgcaggca
      241 gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct gcggagctaa
      301 cggtaacaat accgaccagc gtcgcaatcc acatcgggtc cagcgggtta atgccaaccg
      361 taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcgcagccg ttctgaccaa
      421 tggtcgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacgccc agacgacgcg
      481 tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta aaggcaaacg
      541 tcagcacagg ccatacctta cggaagtact tcagcggact cacgccatta atgcccagca
      601 gaatgccatg cactgcaaac ataatcagca gaccgaggta ggacgcgaca acgaaacttc
      661 ccagtttgat gatgtcttgc aggttagaac ctgcaaccac tttggtcatt agtgccagaa
      721 cgccgtaagg ggtcaactgc atgaccaggc gaaccagttt catcacccag ctttgcaggg
      781 tatcgatagc ggttaagacg cgttcacctt tcggcgcatc atccttcagc agtttcagcg
      841 cagctacacc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc ggattggctc
      901 cggtcagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc ggaacgctca
      961 ggtcagagac tttaccaaca tagttacttt cgatggcgtt cagacgtgca gtttctgcac
     1021 caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc acaccgacca
     1081 gcgccgcaat cagcgtggta aacaaaagcg taccgatggt cagaaaactg attttgccta
     1141 actgagatgc gttatgcaga cgggcaaccg cgctcagaat agaggcgaag actaacggca
     1201 taacgatcat ttgcagcagt tgaacatagc cgttaccaac gatgttaaac cactgtacag
     1261 aatctttaag tatctggctg tcagaaccat aaatggtatg cagggcaagg ccaaaaacca
     1321 cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta tggcgggtct
     1381 gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat ggaaagttca
     1441 tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt gttggcgcaa
     1501 gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt ccagaaaggt
     1561 gatttttgcc tttttagtat tcaacctgat gcttaataag ccgatgaaac tggatctgca
     1621 aggtattgat catttgcgat gaccaacgaa cagcgctgtt ctcgggtaat gaatggggca
     1681 tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg ttaccctttt
     1741 ggggaaccag gataggcaag cggaaacgcc agcggcatgg ccagagcagg ggaacaccgg
     1801 cgggtgtcag catatcggca agtatgtggc ttaaataacc cagcaccatt ccttgtagcg
     1861 catcagccgg aatgaaccag ctttcaggaa ccttaaggta aaaggttgcc agcagcgcaa
     1921 ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg atcggttttg
     1981 atatccactt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc ggtaacaaac
     2041 acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc acgggcgtca
     2101 gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga ccttccgccg
     2161 tcatgataaa actcactaaa ctgtcgatac gtacagtata ggatttttat acagtgcacg
     2221 gaagtggtga cggtgtaact ctttctctta aattgacgat taatccgtca gttgaatgca
     2281 aattcagata aagaaccagg ggcagcgtga tcactgcccc tggtcattaa ccgagaaggt
     2341 cttttgcggt gagttctgtc agcgatgaga gtttgacgtt tgctaataca aaacgtggat
     2401 cattttgcgc ttctggcgca ggaacgacga tggaacgcat gcgggctgct ttagaggcga
     2461 tcatgccatt taccgaatcc tctagcgcta cgcaggtcag tgggtcaacg cccagttttg
     2521 ctgcgcagtc gagatatact tgcggatgcg gcttgctgta aggcagtttt tcggccgagg
     2581 cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc agcatatgta
     2641 gtggtgacgc ggaggccagt cccaccaata aaccttgttc tttgcataac gcaacggctt
     2701 cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag tgaaatggca cgggcaataa
     2761 cccgttctac tacttcctga cggcttggcc cattccacgg ttgccgggcg taccaaagat
     2821 cgaccaccat atcgatgcgt aaacctaagg tgtccggcag ctcgttacga cgggagatat
     2881 ccacccccag gcttgccatc acatccagtt cggctcgatc ccataaaggt tctgagtcga
     2941 taagtaatcc atccatatca aaaattgcag caagaatctg acgcggggtt gacattacaa
     3001 cctctccttt agagcattat gtgtaaggta ttaagctgtg cttatgatta agcgcctatc
     3061 atatcgcctt taaagaatgg gcgaaaacag atatctacag gtaaactaag tgaaacgacg
     3121 cgtcttaatc aaggagaact catgacgtat caacaagctg gacgcattgc tgttttgaaa
     3181 aggattatgg gttgggtgat ttttatacct gcattgatct ctacattgat ttctttgctg
     3241 aaatttatga atacccgaca ggaaaaccag gaaggcatta atgcggtcat gctcgacttt
     3301 actcatgtca tgatcgatat gatgcaggcg aatacgcctt ttttgaatct attctggtat
     3361 aactccccga cgcccaattt taatggtggc gtgaacgtta tgttctgggt gattttcatc
     3421 ctgatttttg tcggactggc gttgcaggat tccggtgccc ggatgagtcg ccaggcgcgc
     3481 ttcttgcggg aaggtgttga agatcaactc attctggaaa aagccaaagg ggaagagggg
     3541 ctaacgcgtg aacaaattga gtcccgtatt gttgttccgc accatactat tttcctgcag
     3601 tttttctcac tgtatatcct gccggttatt tgtattgctg caggttatgt gttcttttct
     3661 ctgcttgggt ttatttaatc aaaaggccgc acggagcggc cattatcctc aacccatatc
     3721 atgctgctaa taatctatcc aatgactgct gagcaataac caaatgctga ccgccaaata
     3781 accttgctcg atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca aggaaatcgg
     3841 caggtagcgg ggatactgac tgatagccgt cataaatttg tggcggctgt tcagtatgca
     3901 gcggtaacat cgccaggtcg cactctcggt caccccagta gcatgccggg tcgaaaatgt
     3961 acgggccatc cggacccagc gcacagttgc cggaccataa atcgccgtgc aacagagaag
     4021 gctgcggttg atgtgaggcg agacgctgct gaatatgctc gacgatggcg tcgatattgc
     4081 cgaaagcgat ccctttttct gctgccagtt ccaactgcca gccaatccgt tgttcagcaa
     4141 aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag agcgcgttat
     4201 cgaaatcgag gccaaattgt ggttggtcac tccattgatg taaacgcgca atttgctgac
     4261 caagaataaa tgcgctatgc gcatccagcg gacgaggtgg gagataatcc atcaccagaa
     4321 aactgtagtc acggtcagcg cctactgccc aaaccttagg cacggtgacg gttttactac
     4381 gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt tccctttcat
     4441 cacatttgac gaaaaagtca tgtcctgcat agcgcaaatg ccatgcggca tggacttctc
     4501 cgccaggcag ttcattacgc agttcgattt cgccttcacc taactgctcg ctcaaaagac
     4561 gactgattgc ctgccacatg atgtctctcc catgttgtct gccagatcat aaggttagcg
     4621 caagaatgcg gtgtaaaaac acgagctaac gcacaccaca gactgattat tgcccaaaaa
     4681 ccatttattt tctgttgcta ttccattcct cccaggtcgt gatggtgatt tcaggatctt
     4741 tgccggttgc actttgggta agccccgaaa ccagttctct aatttcttct tcactttggg
     4801 tactgataag tccaaaagtg ttagtcccca gctcatggat atttccctca tcatccgtca
     4861 tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt tcagtcaatg
     4921 tatcctcatg caacatgacg gttatgacat aacggacaag atcgccactg gccatagttc
     4981 acccctttgt tatcacatga cttttgagca tagtcggaga aacgcgttgc cgacaggttg
     5041 gtgatgattc ccccaatgct gggggaatgt ttttgttagc gggaaaggta agcgtaaatt
     5101 ttttgcgtat cgtcatggga gcacagacgt gttccctgat tgagtgtggc tgcactcccc
     5161 gcagctacgc caaaacgaac catctcttca agagaggcat tttctgccag tttcagtgtc
     5221 atcgcgccga ccatgctgtc accagcgcca acggtactct ggcttttcac cggtggtggc
     5281 accacctgaa tgcagttttc actatcaaca cccagcgctc cttgtggacc cagggaaacg
     5341 acaacccgtt tggccttgcc gctattaacg atttcttgcg cggctttgcg gacatcgtct
     5401 ggctgggtga gttcgcgatt caccagcgca ctgagttctt tttggttagg cttaaccaac
     5461 tcgatgttac caatcgccag tgctgcactt aacgcttcgc cagaactgtc gatgatgcag
     5521 cggatccctt ggttttgcgc agcggaaatc agttgggtta atttttccag cttcacacct
     5581 ggcggcaggc ttccgcttat gaccaggatg gccccggact caatttccag aacttgctct
     5641 tcaagctggc gaaactcatc ttcatttaat gccgcgcctg gcataacaaa acgatactgc
     5701 tcaccgcttg cttccacatg tacgtgtaaa ttctgccggg tccagtcttt ggcttctaca
     5761 gtagcgacgg ggacattttc atccgccaac agtgaaacca ggtgttcgcc ggtcgcgcca
     5821 cccgccggga agatcgctgt ggcactgcct ccaagatggg caatggcgcg ggcgacgttg
     5881 atgccgccgc ccccgggttc gaacaccggt gcggtacagc gcagttttcc ttcgggataa
     5941 atttgcgggg taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt atagatacgt
     6001 accatcattt cctcctatag gctgatttca gtctggcacc gaatcagcgg aaagtgaaga
     6061 aattaacaat atgatttatt ggagct
//
LOCUS       DAZGBC010000233         6015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGBC010000233 DAZGBC010000000
VERSION     DAZGBC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.235"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..1283
                     /gene="ompC"
                     /locus_tag="ACHK1J_004668"
     CDS             156..1283
                     /gene="ompC"
                     /locus_tag="ACHK1J_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7913835.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGYATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1395..2450
                     /gene="apbE"
                     /locus_tag="ACHK1J_004669"
     CDS             1395..2450
                     /gene="apbE"
                     /locus_tag="ACHK1J_004669"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO7913836.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2524..3588
                     /gene="ada"
                     /locus_tag="ACHK1J_004670"
     CDS             2524..3588
                     /gene="ada"
                     /locus_tag="ACHK1J_004670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO7913837.1"
                     /translation="MKNATRLTEDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYADASEALAAGFRPCKRCQPDKANAQQHRLDKITRACRLLEQEMPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRYGGENLAVRYALADCEQGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3588..4238
                     /gene="alkB"
                     /locus_tag="ACHK1J_004671"
     CDS             3588..4238
                     /gene="alkB"
                     /locus_tag="ACHK1J_004671"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO7913838.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4314..5957
                     /gene="yojI"
                     /locus_tag="ACHK1J_004672"
     CDS             4314..5957
                     /gene="yojI"
                     /locus_tag="ACHK1J_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO7913839.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1417 a         1507 c         1650 g         1441 t
ORIGIN      
        1 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
       61 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
      121 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
      181 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
      241 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
      301 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
      361 accagctgac cggttacggt cagtgggaat atcagatcca gggcaacgct ccggaaagcg
      421 aaaacaactc ctggacccgt gtggcatttg ctggtctgaa attccaggat gtgggttctt
      481 tcgactacgg tcgtaactac ggcgttgttt atgacgtaac ttcctggacc gacgtactgc
      541 cagaatttgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaatggct
      601 acgcaaccta ccgtaacacc gacttcttcg gtctggttga cggtctgaac tttgctgttc
      661 agtaccaggg caaaaatggt agcgtaagcg gcgaaggcat gaccaacaat ggtcgtggtg
      721 ctctgcgtca gaatggcgac ggtgtcggcg gatctatcac ttatgattac gaaggcttcg
      781 gtatcggtgc tgcagtttcc agctccaaac gtactgatga tcaaaatggt agctacatca
      841 gcaatggtgt agttcgtaac tacatcggta ctggcgaccg tgctgaaacc tacaccggtg
      901 gtctgaaata cgatgctaac aacatctacc tggctgctca gtacacccag acctacaacg
      961 caactcgcgt aggcaacttg ggttgggcga acaaagcaca gaacttcgaa gctgttgctc
     1021 agtaccagtt cgacttcggt ctgcgtccgt ccctggctta cctgcagtct aaaggtaaaa
     1081 acctgggtgt catcaatggt cgtaactacg acgacgaaga tatcctgaaa tatgttgatg
     1141 ttggtgcgac ctactacttc aacaaaaaca tgtccaccta cgttgactac aaaatcaacc
     1201 tgctggacga caaccagttc actcgtgacg ctggcatcaa cactgataac atcgtagctc
     1261 tgggtctggt ttaccagttc taatctcgat tgatatcgaa caaagggcct gcgggccctt
     1321 ttttcattgt tttcagcgta caaactcagt tttttggtgt actcttgcga ccgttcgcat
     1381 gaggataatc acgtatggaa ataagcttta cccgcgtggc actgctggct gccgcgctct
     1441 tctttgttgg ttgcgatcaa aaaccacaac ccgccaaaac ccacgctact gaagttaccg
     1501 ttcttgaagg caaaaccatg ggtaccttct ggcgtgccag catcccgggc attgatgcca
     1561 aacgcagtgc cgaacttaaa gaaaagattc agacccagct ggacgccgac gatcagctgc
     1621 tttcgaccta taaaaaagat tccgcgctga tgcgctttaa cgactcgcaa agtttgtcgc
     1681 cgtggccagt aagtgaagcg atggccgaaa tcgtcactac ctcgctgcgc attggcgcga
     1741 agaccgatgg tgcgatggat attaccgtcg ggccgctggt gaatctgtgg ggctttggcc
     1801 cggaacaaca gccggttcaa attccgagcc aggaacagat cgatgcgatg aaagccaaaa
     1861 ccggcttaca gcacctgacg gtcattaatc agtcgcatca gcaatatctg caaaaagacc
     1921 tgccggattt atatgtcgat ctctctaccg tcggcgaagg ttatgcggcg gatcacctgg
     1981 cacgcttgat ggagcaggaa gggatttccc gctatctggt gtcggtgggc ggcgcgctga
     2041 acagccgtgg tatgaacggt gaaggccagc cgtggcgggt agcgattcaa aagccaaccg
     2101 ataaagaaaa tgcggttcag gccgtggtgg atatcaacgg tcatggtatt agcacctctg
     2161 gcagctaccg taactattac gaactggacg gcaaacgtct ttcccatgtt atcgatccgc
     2221 aaaccgggcg tcccatcgaa cacaatctgg tatccgtgac ggtgattgct ccgacggcgc
     2281 tggaagccga tgcctgggat actggcttga tggtactcgg gccggagaaa gccaaagagg
     2341 ttgttcgccg ggaagggctg gcggtttata tgatcaccaa agagggcgat agctttaaaa
     2401 cctggatgtc gccgcagttt aaaagcttcc tggtgagcga aaaaaattaa agcgcaagat
     2461 tgttggtttt tgcgtgatgg tgaccgggca gactaaaggc tatccttaac cagggagctg
     2521 attatgaaaa acgccacacg cttaactgaa gatcaacgct ggcaatctgt cttagcccgc
     2581 gacccgaatg ccgacggcga attcgttttc gccgtgcgca ccacaggtat cttttgccgt
     2641 ccgtcttgcc gcgccagaca tgccttgcgg gaaaacgttt ctttctatgc tgatgccagc
     2701 gaggcgcttg ccgctggctt tcgtccctgc aaacgctgcc aacctgataa agccaacgcc
     2761 cagcaacatc ggctggataa aatcacccgc gcttgtcgac tgctggaaca ggaaatgcct
     2821 gtaacgctgg aggccttagc tgaccaggtg gcgatgagtc cgttccatct gcatcgttta
     2881 tttaaagcca cgaccggaat gacgccaaaa gcctggcaac aggcctggcg cgctcgccgt
     2941 ttgcgcgaat cgctggcgaa aggggagagc gtgacgacgt ctattcttaa cgccggattc
     3001 cccgacagca gcagttacta ccgcaaagcc gatgaaacgc tgggcatgac ggctaaacaa
     3061 ttccgttacg gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg tgagcagggt
     3121 cgttgcctgg tggcagaaag cgagcggggg atttgcgcga tattgctggg cgatgatgac
     3181 gcgacactaa tcagcgagtt gcagcagatg tttcccgctg ccgacaacgc gcctgccgat
     3241 ctgacgtttc agcaacatgt gcgtgaagtg atcgccagcc tcaatcaacg cgatacgccg
     3301 ctgacgttac cgctggatat tcgcggcact gcttttcagc aacaagtctg gcaggcactg
     3361 cgcacgatac cttgcggtga aaccgtcagt tatcagcaac tggctaacgc catcggcaaa
     3421 ccgaaagcgg tacgggccgt tgccagcgcc tgtgccgcca acaagctggc tatcgtaata
     3481 ccctgtcatc gagtggtccg tggtgatggc acactttccg gttaccgctg gggcgtgtcg
     3541 cgtaaagcgc aactgctgcg ccgcgaagct gaaaatgagg agaggtaatg ttggatctgt
     3601 ttgccgatgc tgaaccgtgg caagagccac tggcggctgg tgcggtaatt ttacggcgtt
     3661 ttgcttttaa cgctgcggag caactaatcc gcgatattaa tgacgttgcc agccagtcgc
     3721 cgtttcgcca gatggtcact ccaggcggat ataccatgtc ggtggcgatg accaactgtg
     3781 ggcatctggg ctggacgagc catcggcaag gttatctcta ttcgcccatt gatccgcaaa
     3841 caaataaacc gtggcccgcc atgccgcaga gttttcataa tttatgtcaa cgtgcggcta
     3901 cggcggcggg ctatccagat ttccagccag atgcttgtct tatcaaccgc tacgctcctg
     3961 gcgcgaaact gtcgctgcat caggataaag acgaaccgga tctgcgcgcg ccaattgttt
     4021 ctgtttctct gggcttaccc gcgatttttc aatttggtgg cctgaaacgt aatgatccgc
     4081 tcaaacgttt gttgttggaa catggcgatg tggtggtatg gggcggtgaa tcgcggctgt
     4141 tttatcacgg tattcaaccg ttgaaagcgg ggtttcatcc actcaccacc gactgccgct
     4201 acaacctgac attccgtcag gcaggtaaaa aagaataaaa ataagaatta ttattgctgt
     4261 gcgcgcgaag atgtttaaac tgcgggctgt aattcattgt ccgggttttc tgcatggaac
     4321 ttcttgtact tgtctggcgg cagtatcgct ggccatttat cagtgtgatg gcgctaagcc
     4381 tcgccagtgc ggcattaggc attggcttaa ttgcttttat caaccagcgc cttatcgaaa
     4441 cggcggatac cagtctgctg gtgttgccgg agtttctggg attattgctg ctgttgatgg
     4501 cagtcactct cggatcgcaa ctggcgctca ccactttggg gcatcacttc gtttaccgac
     4561 tgcgtagtga atttatcaag cggattctgg atacccacgt cgagcgcatt gaacaactcg
     4621 gtagcgcctc gttgctggcg ggattaacca gcgatgtgcg caatatcacc attgcttttg
     4681 tgcgtctgcc ggaactggtg caggggatca ttctcactat cggttcagcg gcgtatctgt
     4741 ggatgctgtc gggcaaaatg ttgctggtaa cggctatctg gatggcagtc accatctggg
     4801 gcggttttgt gctggtggcg cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag
     4861 acaagctgta cacggatttt caaactgtac ttgaagggcg caaagagctg actctgaacc
     4921 gggaacgcgc cgagtatgtg tttaacaacc tctacattcc tgatgcgcaa gagtatcgcc
     4981 atcatattat tcgcgcagac accttccatc ttagtgccgt gaactggtca aacatcatga
     5041 tgctgggcgc aatcggcctg gtgttctgga tggcgaacag cctcggttgg gctgatacca
     5101 acgttgccgc gacctattcg ttgacgcttt tattcctgcg tacgccgctg ctttcggcgg
     5161 ttggcgcatt gccgacgctg ctgacggcgc aggtggcgtt taacaagctg aacaaattcg
     5221 cgctcgcgcc tttcaaagca gagtttccgc gcccgcaggc gtttcccaac tggcaaacgc
     5281 tggagctgcg taacgtgacg tttgcttatc aggataacgc gttttccgtt ggtccgatta
     5341 atctcaccat caaacgtggc gagctgctgt ttctgattgg cggcaacggt agcggaaaat
     5401 cgacgctggc gatgttgttg acgggcttgt atcagccaca aagcggcgaa atcttgctgg
     5461 atggcaaacc tgtcagcggc gaacaaccgg aagattatcg caaactgttt tcggcagtgt
     5521 ttaccgatgt ctggctgttt gatcaactgc tggggccgga gggtaaaccc gctaacccgc
     5581 aactggttga gaagtggctg gcgcagctga aaatggctca taagcttgag ttaagcaacg
     5641 ggcgtattgt taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg
     5701 cgctggcaga agaacgcgat attatcctgc tggatgaatg ggcggcggat caggatccac
     5761 acttccgtcg tgagttttat caggtgttgc tgccgctgat gcaggagatg ggtaaaacta
     5821 ttttcgccat cagtcatgat gatcattact ttatccacgc cgaccgcctg ctggagatgc
     5881 gcaacgggca acttagcgag ctgacgggcg aagagcgcga tgccgcttcg cgtgatgccg
     5941 ttgcccggac ggcataatat tacgacgctg acctggcgga ctgctgccgc caggtcaata
     6001 tgctaaaaat ccctt
//
LOCUS       DAZGBC010000234         5999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGBC010000234 DAZGBC010000000
VERSION     DAZGBC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.236"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(177..1142)
                     /gene="alx"
                     /locus_tag="ACHK1J_004673"
     CDS             complement(177..1142)
                     /gene="alx"
                     /locus_tag="ACHK1J_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO7913840.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(1426..2412)
                     /locus_tag="ACHK1J_004674"
     CDS             complement(1426..2412)
                     /locus_tag="ACHK1J_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7913841.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKQ"
     gene            complement(2491..3183)
                     /locus_tag="ACHK1J_004675"
     CDS             complement(2491..3183)
                     /locus_tag="ACHK1J_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO7913842.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATLFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARISAVIDAKIFHRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(3260..3763)
                     /locus_tag="ACHK1J_004676"
     CDS             complement(3260..3763)
                     /locus_tag="ACHK1J_004676"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO7913843.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            3848..4984
                     /gene="rlmG"
                     /locus_tag="ACHK1J_004677"
     CDS             3848..4984
                     /gene="rlmG"
                     /locus_tag="ACHK1J_004677"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO7913844.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAREMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            5268..5582
                     /gene="higB"
                     /locus_tag="ACHK1J_004678"
     CDS             5268..5582
                     /gene="higB"
                     /locus_tag="ACHK1J_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO7913845.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            5579..5995
                     /gene="higA"
                     /locus_tag="ACHK1J_004679"
     CDS             5579..5995
                     /gene="higA"
                     /locus_tag="ACHK1J_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO7913846.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         1567 a         1566 c         1473 g         1393 t
ORIGIN      
        1 tccctcagct tatcccttgc ggctaccggg tcgggcgcaa gggaacgctt cggcgatttt
       61 gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt acataattaa
      121 tagctcacct ttgtctggaa tgagttatct ctagtaatgg cttaatatac agcgaatcat
      181 tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa cgtcatcacc
      241 aggatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa gtcgacaatc
      301 agcatcttga taccgataaa taccagaatc accgccaggc catatttgag catcgagaaa
      361 cgctccgcga cgcccgccag caggaaatac atcgcacgca ggccgaggat tgcaaagagg
      421 tttgaggtca gcacaataaa cgggtcagtg gtcacggcga agatagccgg aatgctatcc
      481 acggcgaaaa tcacgtcgct caattccacc agaatcaata ccagcatcag cggtgtggcg
      541 tacaacaagc cattcttgcg cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc
      601 aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc atcttcatgg
      661 gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata caggatccag
      721 tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag aacaatcgcc
      781 ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga gaaatagctg
      841 aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat cagataaccc
      901 gtgagaaagg ccagtgcctg tggatcggca acggcgcgac cttcggtttg caccagatac
      961 caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga ccaggccgca
     1021 gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag gtcgatagcc
     1081 agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc gacagtattc
     1141 atagaagttc cttacacata aaaaaaacgg ccaacgtcgg aagacgtcag ccgctgcttt
     1201 ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg aatacgtctg
     1261 cgtattcctt tcgggttgcc cggtgaccgg atgtgatttt tcacacatcg taatgacgat
     1321 cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac cattcaccca
     1381 gtcaatggtc tgttacatgc cttttacact gctttacggt aacaattatt gttttacgct
     1441 atctgccgga aaaatcaccc ctgtctggcg gcggatctct gtcagcaatt tggcggtgat
     1501 gcgactgacc gccagtcccg gatgatccac cagatgctca tccaccaggg tagcgaacag
     1561 ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct gcatctggct
     1621 gccacgcggc acaaagcata ctttctggca ttcagacagt ttttcaatca ccagcgatcc
     1681 cgcttcgccc tgaatttcgc ttgccaggac agaatcactg acttttgagt gctgcaacgt
     1741 aacgctgaaa tcaccgtaat ccattaccac cacgccgtgg gcatctacgc cgcttgccag
     1801 caaactggcg gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg ccgacgccag
     1861 gcagtaaaag ccgatatcca taattgaacc gttagagaat gccggattaa aggtgttggg
     1921 attctcaccg tccaggtaac gttgataccg cgaggaatac tggcaatagt tgaaaaaaac
     1981 tttccgcagt ttgccgactt tcggcagcgc ctggcgcaac aaatgaaagt tcggcaggct
     2041 ggcggtttta aatgcctcaa acagcaccac ctgattttcc cgcgcacagg caatggcggc
     2101 atccacttcc gccagattcg acgccagtgg tttctcgcaa atcacatgaa ttttatggct
     2161 aaggaaaagt tgtgtctggg aaaagtgcag ggaattcggg ctggcaatat acaccgcgtc
     2221 aatggcatcg ctttctgcca tcgcttccag cgacgtaaac agatgctcga cagaaaaatc
     2281 attggcgaag tgctgggcct gttcaaggct gcgggaatat acggcggtta acttgtattt
     2341 accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac caatcacagc
     2401 gaaacgtatc ataacgcgtg gatactccat aaagggattc tgccgtcaat gtagcacgcc
     2461 tttttcatct tcgacgtgcg cggatacgca ttatttctgc gctggacaac catgctcact
     2521 aaacggacca ataatgaccg acggcccgag atattttggc tgacgatgga agattttcgc
     2581 gtcaatcacc gcgctgattc tggcgagttt ctcccgtaac tgagtataaa aaccgtgctt
     2641 catattcagg tctggtgcgt taaacccgat agcatcgatg ccgtattgtt ttgccagcca
     2701 gatggcgcgt tgattatgaa attcctgcga gataatggtg atatggtttt ccccaaacac
     2761 ctttttggca cgcactaccg aatccagcgt tgagaatccg gcatagtcgc agaagatcac
     2821 tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgatgctt catcgtaatt
     2881 tttacgtccg ttatcgccac tgactaacag ccatttcact tttccggcgt ggtacaactc
     2941 tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctggccc ccagcaataa
     3001 gcccacgtta cgcgccggaa cgacgttgac gtcgctccag gtcagctgtt tgctggcgtt
     3061 gaccattacg cgatcggcaa taaaaagggt cgctcctgcc acaagtaaca gtaagcaggc
     3121 aatctttaga gtccggcgag aaaaacaaat acggagaaga aggcgggcaa atgcgcgcag
     3181 catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa gaaagaaaag
     3241 ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc gttagccaca
     3301 gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac aattgataga
     3361 acgctttgtt atgctctttc tccttcaggt gcgccaactc gtgcaccacg atcatgcgca
     3421 aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct tttgctttca
     3481 gcttgccgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt tttagcacat
     3541 ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga aactgatttt
     3601 tcagatcctg agtatattgc cagagggctt tatcggtggc gtagtcgtgt gttcccggat
     3661 agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc acctgggaaa
     3721 gtagctgctc gggataaccc tgaagataag taagattgct cataaccgcc cacgaaaaag
     3781 agaaaaaggg tatactcacg caccctttgc aggggcaacg tcgaaatttt atcattcagg
     3841 agggccgatg agccacttag acaacggttt ccgttcactg acactacaac gtttcccggc
     3901 gacggatgac gttaacccgc tacaggcgtg ggaagcggcg gatgaatatt tgctgcaaca
     3961 gttggacgac acagaaatcc gcggcccggt gttaatcctg aatgatgctt ttggtgcgtt
     4021 aagctgtgtg ctggcggaac ataagccgta cagcattggc gactcataca tcagcgaact
     4081 ggcgacgcgc gagaatttac gccttaacgg gattgatgaa tcgagcgtga agtttctcga
     4141 cagcaccgcc gactacccgc aacagccggg cgtggtgctg atcaaagtac cgaaaacatt
     4201 ggcattgctg gaacaacaac tgcgtgcgct gcgcaaagtg gtcacgccac aaacacgtat
     4261 tattgccggt gccaaagccc gtgacatcca cacttccacg ctggaactat tcgaaaaagt
     4321 gctcggcccg accaccacca cgctggcatg gaagaaagcg cgcctgatta actgcacttt
     4381 caatgaacca ccgctggccg atgcgccgca gaccgttagc tggaagctgg aaggtactga
     4441 ctggactatc cacaaccatg cgaatgtctt ctcccgcacc gggctggata tcggcgcgcg
     4501 cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc tcggttgcgg
     4561 taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag tggtgtttgt
     4621 cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca acatgccaga
     4681 ggcgttggat cgctgcgaat ttatgatcaa caacgcgctc tccggcgtgg agcctttccg
     4741 ctttaatgct gtgctctgca acccgccgtt tcatcagcaa catgcgctga ccgataacgt
     4801 cgccagggag atgttccacc acgcccgccg ctgcctgaaa atcaacggcg aactgtatat
     4861 cgttgccaac cgtcacctgg attacttcca taaactgaag aagattttcg gcaactgcac
     4921 caccatcgcc acgaataata aatttgtggt gctgaaagca gtgaagctgg ggcgtcgtcg
     4981 gtaagattga cagctacctg ccagataagg cgtaaacgcc ttatctggca tacgtttagc
     5041 acattacccc caggtcggat aagatgcggc aagcgtcgca tccggcaatg tctgcccagt
     5101 tctggaatcg cgctccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt
     5161 tagcgttgtt caacgtttat tcttccgtca cacagataaa ttcatccgtt gccaatctgt
     5221 caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg cacctgataa
     5281 ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg gagttggtgg
     5341 ctctagggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta aaagcagtat
     5401 tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat gttgggggca
     5461 atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac atacgtgaag
     5521 ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat
     5581 gattgcgatt gccgacatct tgcatgcagg agaaaagctg actgctgtgg caccttttct
     5641 ggcgggtatt cagaacgagg aacaatacat ccaggcgctg gaactggtag atcatctgct
     5701 gctcaacgat cctgaaaacc ccttactgga tctggtgtgt gccaaaataa ccgcgtggga
     5761 agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat
     5821 agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc tgccggaaat
     5881 tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca
     5941 cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg attaacgcg
//
LOCUS       DAZGBC010000235         5969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGBC010000235 DAZGBC010000000
VERSION     DAZGBC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.237"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            309..1472
                     /locus_tag="ACHK1J_004680"
     CDS             309..1472
                     /locus_tag="ACHK1J_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO7913847.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNIFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ENELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1477..3624
                     /locus_tag="ACHK1J_004681"
     CDS             1477..3624
                     /locus_tag="ACHK1J_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO7913848.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKKLSGKQQSEESRQAAS"
     gene            3684..4931
                     /locus_tag="ACHK1J_004682"
     CDS             3684..4931
                     /locus_tag="ACHK1J_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO7913849.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5216..>5969
                     /locus_tag="ACHK1J_004683"
     CDS             5216..>5969
                     /locus_tag="ACHK1J_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913850.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLAEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEGE
                     QKPVQPEGGQEAGKTDVSSTGSTEAGSGSSSEQVPEVNDQNSNGKPDAQDAKDAEDAA
                     KALVEAAEAKQAEAEGALTEANKDGAINPDEHT"
BASE COUNT         1506 a         1277 c         1724 g         1462 t
ORIGIN      
        1 cataaactgc aaggattgta agggtttttc aggttttcaa taaaattgaa gtatctcaca
       61 tttttattat cttctgtcag cgcggaagat aatggcggta gcttggatca attccggtgt
      121 ctctgcattt caattttact caatatttta cccggttgca tcatgatgcc tgattatatt
      181 tcccgcgtgt cccggaggtt cgggtgaaga gaatattatc tttctgtttt cttttctctg
      241 tccacaacgt gttcgcttct ccactgccgg ccctcctgag ggatgctctc attcatgatc
      301 catcagtgat ggaggcaagg gcagaagcca cctctgcccg ttcccgtgtg gagcaggctc
      361 gttcagcaca ctggccggtc gtcacagcaa caggcagtaa actcctttcc cagagtcacc
      421 gctacgacta tgactacgac tctgaagata ttttgcccgg tatccgcggt gaaatgaata
      481 tattcgcttc gggagctatt gaggcggatg tcagccggag tgagtcggaa gccgagtatt
      541 accgttataa agttgatgag gctggcgagg aaacaattcg ctcctttgtt tcattatatc
      601 ttgatgcgct aagggaaaag caatccattg cggtacttga acagagtctc tcccggcata
      661 acgcaattct taatgatctt aacaccatca gtattcacga taccgggcgg gaatctgaat
      721 ttgttcaggc cgaagcccgc aggctgatgg tccggcagca gataaatacc cgcacccgag
      781 tgctcaaaac cacgctggga aaactttcga cctggaccac aaatccggta accgaagccg
      841 atcttgaaaa tcctttttcc cggatgacag agtccagatt gttaactgat tttacgcaga
      901 cgcctcagaa agggaatccg tcgtggcttg ccagtcaggc tgatgtagag agtaaaaaag
      961 cggcgctgaa agcgcaggag cttgcccggt atccccgggt ggatttaacg ggatcagtga
     1021 cccgggatga caggcagata ggtgtcagtc tgtcctggga cctgttcaac cgtaatgcca
     1081 gttatggtgt cagtgaaaag gccgcgcaga tagctgcagc taccggacga ctggactctg
     1141 ttgcccgaat gattgatgaa accggacgat tatcgctgat aacggtcaga caaagtcgga
     1201 tggaaatgga aacgctcaga cgtcaggagc aggcctctgc cagagttgtg gacttttatc
     1261 gtcttcagtt ccgggtggca agaaaaacac tgattgaatt actgaatgca gaaaacgaac
     1321 tgtacagcgt cgggctttcc cgggttcaga cagaggatca gatgctccac ggaatgctgg
     1381 attatctgta ttcccaggga atgctcctga aatggagcgg agtgaatctt tctggtgcag
     1441 aagaaaaagt aacagggcag aaggacagat aaataagtga agagtatttt tgaaggatta
     1501 tcacaggtaa cggcattact gggtacacct gtatctgctg agacgctggc tgccggtaca
     1561 gtccggacag atgtctccgg gattgatttt cgttctgttg gcgagtttct tcgcagtgaa
     1621 gggtttgata atcatctttc ccggcgggct ccggaagata ttccttcact tgcggttcct
     1681 gtgcttcttt tactgaatgc acaggaagcc attgtggtgg tgcgtattga gggtgccggc
     1741 ctggcccggc agtatcatgt actccagccc gggggactcc gtaagacact ttctcacgat
     1801 gaattaatgg ctgtttattc aggttactgc tggtttgttc agccacaggc aacacaggat
     1861 cgccgttcgg aactgccgga gtatgacatg accggggcat ggttctggcg ggtactgtgg
     1921 cggttcaggc catattacgg acaggtcatt cttgcgagtt ttatcattaa ctttcttgcc
     1981 ctggtcagtt ccctgtatgt catgaacgtt tatgaccggg tcatacccaa tcgctcctgg
     2041 gaaacgttat ggatgctgag tatcggggtg attgcagcca ttctgtttga gtgtgctgca
     2101 aagttaatcc ggagctatct gacggatatt gcgggtaaga aggcagacct gattatcagc
     2161 tcagcactgt taaggcgggt gatggcagtg aaaatgtcgg acaggccggc gtcttccggt
     2221 tcatacgcca gcaatttgcg ggaatatgag tctgtcaggg agtttatgac cagcgccacg
     2281 ctgctggctt ttgtggattt gccgtttatc ctgcttttca ttggtgtcat cgggcttgtg
     2341 gggggctggc ttgctgttgt tcctcttaca ctggttccgc tggtgattct ggcaggaata
     2401 ttactgcagc gaccgctttc gcgtcatatt aatgcctcca tgcgggaatc atcgcagcgc
     2461 cagggattac ttgtggaggc actggacgga atagaaaccc tgaagctgaa taatgcagtt
     2521 aactgggtcc agcgccgctg ggatggatat acagccagtg tcgcccgctc gtccatacgg
     2581 gtaaaaaata ccagtaatgt cataatctgc ttttccacag cagttcagca actgaatact
     2641 gttctgctgg tcattctggg gacgtacctt attcacagtg atgacccaca caaccgaatc
     2701 accatggggg cactgattgc cgcagttatt ctttccggtc gtgcgctggc accgcttggt
     2761 cagatagcat cactggccac ccgttttcag caggcccgcc tggccatgca gggggtgaat
     2821 gacattgtcc gccgtccggt ggagcgggaa agtcagacac attatctgac agtgaaggat
     2881 atccagggag agtttcgttt cagtgatgtc tccttttctt ataagcccgg attacccgac
     2941 gcattgtgcg gtctgtcgct ctgtatccgg cccggagagc gggtgggtat tattggctca
     3001 ataggttgtg gtaaatcgac gctgctgaaa atgatggtgg ggctgtaccg tcagaacagg
     3061 ggacaaatta ctcttgacgg ggttgcgatg cagcaactgg acccctgttt tgtccgtgat
     3121 aaagtgtttc ttctggaaca gaatccccgg ctttttctgg gaactctgcg tgaaaatctg
     3181 cagctttcgc gtctgccggg aagcctgtcg gatgtggata tgattaatgc agccggacgt
     3241 ttcgggtttg accgttttat ccggcatcat ccccgcggac ttgatatgca gacaggggaa
     3301 ggcggtcagg gactgtcagg aggacagagg cagatggttg ccctgacgag aatggtactg
     3361 ggttctcccc gggtggtact gcttgatgag cccacgacag gtctggacct gcagactgag
     3421 tctgttgttc tgaaaggtct gagccactgg agccgtggac gcacaatggt ggtggtgaca
     3481 catcgtctgc aggttctgaa tattgttgac cgtattgtcg tgatgaatga agggcgggta
     3541 atgatggacg gtccccgtga tgaaatactg aaaaaactgt cagggaaaca gcagagtgaa
     3601 gagagcagac aggcagcttc ctgaacgtca accggataag aaaaaagagc agatatgttt
     3661 aacagtttgt ggaccgcagg gatatgacag aaaaaacgga acggaataaa gcgtcgtcag
     3721 aggtggtcag gcagtcagac ctggcgctgt tgacagatgt acaggcagcc cttcagcagg
     3781 aaaagcatca tgcccttttc ctgatggtga tttttctgct ggtactggta gtgacgtttg
     3841 ttatctgggc atggaacagt cctctggatg aagtgacccg tggtcagggg agtattattc
     3901 ccggcagcag ggagcaggtt atacagaccc ttgacccggg tatactgaaa tcacttgagg
     3961 tcagggaagg cgatattgtt gagaaaggtc aggtactgct gacactggat gatacccgga
     4021 gctctgccat gctgagggaa agtgaagccc gggtgaataa cctggaggcc gtcagggcaa
     4081 gactccgtgc agaagcttat tccgaatcac tgacatttcc ggataacgtt cctgaggatt
     4141 tgcgggagcg ggaaagtacg gtctattttc tccggaagac ggagctggcg cagagcattg
     4201 ccggtctgaa gcagagtaag gcgctgctgg acagggaaat agcaatgacg cggccaattg
     4261 tgcgtgaagg ggcaatgtcg gaggtcgaac tgttacgtat gcagcgtcag tcagcggagc
     4321 tgcagttaca gatggatgag aaacagaata aatacctgac ggaagccggt gctgaactgg
     4381 tgaagacaga agcagagctt gctcaggcaa aagagaatat ggctggcaga gccgacccgg
     4441 ttgagcgttc acgtctcagg gcccccctga gaggtatcgt aaaaaatata cgggtaaaca
     4501 cactgggagg ggtggtttct gcaggacagg acataatgga aattattccg ctggaagacc
     4561 agttgctgat tgaagcttat ataaatccac gggatgttgc ctatgtcaga acagggatgc
     4621 cggcacttgt aaaactgacg gcttatgact atgccattta tggtggtctt gacggcgtgg
     4681 tgacactggt aagtccggat actttacgtg accagaaacg gccgggagat ttaaaactgg
     4741 acccgaatga agcttattac cgggtactgg ttaccaccag taataattat ctgacagaca
     4801 ggaacggaaa aagattgcca gttattcctg gcatgatagc cagcgtggat ataaaaaccg
     4861 ggcgtaaaac ggtatttcag tatctgatta aaccgataac cagaatgaag caggcacttc
     4921 aggaaagata ataaaaaggt cttccgggga aagtatttta aatcaatttt aaacgggcag
     4981 aatgatttca ctttattctg cccgggtcat tgcgcatgcc tgtatgtgat ttggggctga
     5041 atataatata aatgttatat ctgtcagggg aaaataaatc tgaaaaagcg gaatgattaa
     5101 ggaggtccgg ccagagaaat aaattatctg taatctgatt cgtttttatt taatgactcc
     5161 ctttagcgga taaaacataa gagcatactg gttttattaa cctgaggata cttatatgaa
     5221 aatgattttc accggcaagg tcagtggaga aaaaacagta ctgacagccg gagcccgtca
     5281 tactgtaaag gcacaggccg gggaacagta tggactggtt gatgaggtga caggtctggt
     5341 cccggatggt gtggaagcgg accgttctgg tgatgattta attctgcgta aaaaggaaga
     5401 tgatacagaa atccggattg agggattctg ggaagaatgc cagcctggtg aaacacagtg
     5461 tacagccgtg tttaatgttg tgggtgaaaa tggccaggta actgaagctg tcctgaccca
     5521 ggatggtcct gtactggaga attttactgc agggcaaagt ggcacactgg ctgaaggtat
     5581 caataacggg attatctggg gggggattgc ttttggtgca gggctggcgg ctattgcact
     5641 ggctgccgga ggtggtggtg gaagcagtcg tcataagcgt gcaactgaag gtgagcagaa
     5701 gccggttcag cctgaaggcg gtcaggaggc tggaaaaaca gatgtatcat ccacaggctc
     5761 aactgaggcg ggaagtggaa gctcttccga gcaggtaccg gaagtgaacg atcagaacag
     5821 caacggcaag ccggatgcac aggatgcgaa ggacgcagaa gatgccgcga aggcgctggt
     5881 tgaggcagcc gaagcgaagc aggctgaagc ggaaggtgcg ctgacggagg ccaataaaga
     5941 cggagccatt aatccggatg agcataccg
//
LOCUS       DAZGBC010000236         5930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGBC010000236 DAZGBC010000000
VERSION     DAZGBC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.238"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..987
                     /gene="espL4"
                     /locus_tag="ACHK1J_004684"
     CDS             <1..987
                     /gene="espL4"
                     /locus_tag="ACHK1J_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO7913851.1"
                     /translation="IFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKN
                     VVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDALP
                     LLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISA
                     SDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLAN
                     NSNEYPGLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMGFWQHKAPQKYSAFEL
                     ACELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(956..2692)
                     /gene="aceK"
                     /locus_tag="ACHK1J_004685"
     CDS             complement(956..2692)
                     /gene="aceK"
                     /locus_tag="ACHK1J_004685"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO7913852.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPEHGWEALLMRVLSDL
                     PLRLPWQNKSRDINYIIRHLTETLGPENFSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDYGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRTLQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2876..4180)
                     /gene="aceA"
                     /locus_tag="ACHK1J_004686"
     CDS             complement(2876..4180)
                     /gene="aceA"
                     /locus_tag="ACHK1J_004686"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO7913853.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4210..5811)
                     /gene="aceB"
                     /locus_tag="ACHK1J_004687"
     CDS             complement(4210..5811)
                     /gene="aceB"
                     /locus_tag="ACHK1J_004687"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO7913854.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1426 a         1536 c         1475 g         1493 t
ORIGIN      
        1 atttttaatt cattgtcaga gccaggatat gaaggattac tctcgaaaaa aaatctcatg
       61 catattctgg aggcaaaaga taaaaatggt ttttctggat tatttttagc gatatcacgt
      121 aaggataaaa atgttgtaac ctcgattctg aacgccttac ctaaactggc cgcaacacat
      181 catttagata acgaacaagt gtataaattc ctgagtgcca aaaatagtac gtccagccat
      241 gttttatacc atgttatggc gaatggtgat gccgacatgc tgaaaattgt tttggacgcg
      301 ttacctttgt taattcgcac atgtcatttg actaaagaac aggtactcga tctcctgaag
      361 gcaaaggatt tttatggttg cccaggacta tacctggcga tgcaaaatgg acatagcgat
      421 atcgtgaaag ttattcttga agcattgccc agcctggccc aggaaattaa catttcagct
      481 tccgatattg tagatcttct gaccgctaaa agtcttgcgc gcgacacggg tttgtttatg
      541 gccatgcagc gcggacatat gaacgttatt aatactattt ttaacgcatt acccactctg
      601 tttaacacgt ttaaattcga taaaaaaaat atgaagcccc tcctcctggc aaataattct
      661 aatgaatatc ccggtttgtt ttcagcgata cagcataaac aacaaaacgt tgtagagacg
      721 gtttatcttg ctttatctga ccatgcacgc ctgtttggat ttaccgctga agatattatg
      781 ggtttttggc aacacaaagc gccacaaaaa tattctgcct ttgagttggc ttgtgaattg
      841 ggtcaccggg ttattgctga attaatcttt aatacattaa ataagatggc tgaaagcttt
      901 ggctttacgg ataaccctcg atacattgcg gagaaaaatt atatggaagc tttactcaaa
      961 aaagcatctc cccataccgt acgctaaatc tttgcctgcg ccgatacgca taaacatctt
     1021 ccacatgtcc ttcacgaatg cggttttgta gtgtgcgcca gtaatcagcg cggaacaggt
     1081 cggcgtgcat ctcttcaaag agtggcccaa tgcgggggtc ggcgcatagc cagtggcgga
     1141 actcttccgg gaaaacatcg cccggcgaga cgctgtacca cggttcgctg gcaagttcgt
     1201 cttccggata gcgcggcggc gggatgtcgc ggaagttcac ttccgtcatg tagcaaattt
     1261 catcgtaatc ataaaaaacc acacgcccgt gacgggtgac accgaagttt ttaaacagca
     1321 tgtcgccagg gaaaatgtta gcggcggcaa gctggcgaat agcgttaccg tattcttcaa
     1381 tggcgtcacg caactgctga ccttccactt gttccagcca gatattgagc ggcaccatcc
     1441 gccgctcaat ataaagatgg cgaatcacaa tttgttcgcc gagatcggtg attttttccg
     1501 ctgcttcctg gagcagtaat tccattaatg ccggggaaat atgccgcttc tccagcacaa
     1561 agttttcaaa ctcctgggtg tccgccattc ggcccacgcg atcatgctct ttcaccagtt
     1621 gatagcaggc acgaacgtga gcggcagaca tctctttctg cggcgcgaac ctgtctttga
     1681 tgactttgaa tacccgatca aagcccggta gcgtaaacac caacatcacc atgccacgaa
     1741 tacccggcgc ttcaatgaac tgctcattac agccctgtag ataaacgaga tattcgcggt
     1801 agctctcggt tttggcatgc ttctggcagc cgatagccat atacaattca gcggtggttt
     1861 tacctggcag aatttcccgt aaccactcga ccagcgctgc gggcagcggc gcataaacca
     1921 taaaataaga acgcgcaaag ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg
     1981 tatcaataaa taactcgccg tagtccgtct ggtggatcgg cagcaaaaat ggcaatgtgc
     2041 cggaaggcgt gatcagtttg cctaccagcc aggcggcttt attgcggtaa aacagttcgt
     2101 tcgccacctg taaatgactt ttggagaagt tttctggccc caacgtttcc gtcagatggc
     2161 gaataatata attgatatca cggcttttat tctgccaggg caggcgcagc ggcaggtcac
     2221 tgaggactcg catcagcagc gcttcccagc catgctcggg gtagaagtct ttcgccagtg
     2281 ggcgcggaat ggtacgaaag cggcgctctg gctgagagct aaagataaaa agccgctcgg
     2341 gagtaagcga gcggtggtca aataaccgac agtacacgga gttaaaaaag ctctccgcaa
     2401 tctcgaagcg cgggtaatcc ggcaacagcc gggtgtaatg ctctttgacg cgcagtaaaa
     2461 attccgcgtc cgtgctttgg ccgttggtaa tgcagcgcag ttgctccacg accagaccaa
     2521 cgtgatgatc gtaaagatgg atacggtttt tcatcgcctg ctggacagca tgccagtcgg
     2581 cctgttcgaa acgctgctgc gccccggaag tcacttcgag gaatcgacca tactgagcgt
     2641 cgaagccttg caaaatggtt tgagcaatca gtaattccag gccacgcggc atttagcgcc
     2701 ctcatcagga gcagagaatt gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg
     2761 caacggttgt aggcatgata agacgcgcaa gcgtcgcatc aggcatcgtg cactgattgc
     2821 cggatgcggc gtaaatgcct tatccggcct acagtcagca acgtttgtag gttgcttaga
     2881 actgcgattc ttcagtggag ccggtcagcg cggtgactga agacgtgccg ccctgaataa
     2941 tggtcgtcac tttatcgaag taacctgtac ccacttcctg ctggtgagat acgaaggtgt
     3001 agccatcttt cgcggcggcg aattccggtt gctgcacttt ctcaacgtag tgcttcatac
     3061 cctcgccctg ggcataggcg tttgccaggt cgaacatgtt gaaccacatg ctgtggatcc
     3121 ccgccagggt gatgaactgg aacttgtagc ccatatctga cagctgctgc tggaagctgg
     3181 caatagtttt gtcgtcgagg tttttctgcc agttgaacga cggcgagcag ttataagcca
     3241 gcagtttgcc cggatatttc gcgtggatgg cttgtgcaaa gcgacgcgcc agttccagat
     3301 ccggcgtgga ggtttcacac cataccaggt cagcatatgg tgcatacgcc aggccacggc
     3361 tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc ttcactggta cgctcgccgg
     3421 taataaattc gctgtcatac gggtcgcaat ctgaggtgat cagatccgcc gcatcagcat
     3481 cggtacgtgc aaccagcagg gttggaacgc ccgtcacgtc agctgccaga cgcgccgcga
     3541 ccagtttctg aatagcttcc tgagttggca ccaaaacttt gccgcccatg tgaccgcatt
     3601 tcttcactga cgccagctga tcttcgaagt gaactgccgc tgcaccggct tcaatcatcg
     3661 ctttcataag ttcaaaggca ttcaggacac cgccaaaacc ggcttccgcg tcggcaacga
     3721 tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc aatgcccgcg gaccattgga
     3781 tctgatccgc acggcggaaa gtgttgttga tccgctccac cacagctggc accgagtttg
     3841 ccggatagag cgactgatcc ggatacatgc tggccgccag gttagcgtcc gccgctacct
     3901 gccatcccga cagatagact gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc
     3961 cagtcagtgc accgaggctg ttgatgtagc cttttttcga ctcgccatgc agcagacgcc
     4021 acattttcgc cgcgcccagt tgcgccagcg tgcattcagg attgactgaa ccgcgtaatt
     4081 tcaccacatc ttccgcgctg tatgggcgag taatgccttc ccaacgcggt tgagtccact
     4141 ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc tccatagtta
     4201 tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc aggaaatcaa ttaactcatc
     4261 ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca aaacgcccct gggagaaacg
     4321 ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct ggcggaacaa
     4381 ggctttggtc accggtttgc cattgctcaa cgttttttga tgatggatcc actgccagat
     4441 cgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa tcggcacgca
     4501 gccgttgccg gagatccatg cttcgatgta ctgcacagcc acgcgaatgt tggcgcgcat
     4561 accttcttcg gtgcgttcac catcacaagg tgccagcagc tgatcggcag taatcggcgc
     4621 gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt cgttaaatac
     4681 cgccattgcc gtatccgcaa ggcctgggtg agcgatccat gtgccatcgt gaccgttatt
     4741 ggcttccagc gatttatccg cttttacttt gttgagcact tggttattgc gctcttcatc
     4801 tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt
     4861 tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca ctgcctgtct
     4921 gtctggcagg acgcgatcgg gatagttttt caacgttttg atataactga agatgtaatc
     4981 ccaacgaccg cagttcagac caacaatatg gtcacgcagt gcgtgaagga tttcgtccat
     5041 ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag
     5101 attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt cctgccagga
     5161 ctgggttttc ggcagataga aatagggacc gctgcccttt gccaacagtg cctgatagtt
     5221 gtggaagaaa tagagcgcaa aatcaaacag gctaccgggg attgcctcac cacgccaggt
     5281 gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg ctggattggg
     5341 cttgagctgg taaattttgc ctgcttcatt cgtgtaactg atggtgccgt taaccgcatc
     5401 gcgcaggtta atttgcccgt cgatcacttt gttccagtct ggtgccagtg aatcttcgaa
     5461 atcggccata aagactttca cattggcgtt cagtgcgttg atcaccatct tgcgctctac
     5521 cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc gaattttcca
     5581 atcagcatcg cgaatggaag ctgtttccga aataaaatca ggcaacgttc cgttatcaat
     5641 atcttgctgc tgctgaatgc gcgctgccag aagtttattg cgttgtggcg taaaatgcgt
     5701 caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc
     5761 atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct
     5821 cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg cgcttttcgt
     5881 tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc
//
LOCUS       DAZGBC010000237         5925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGBC010000237 DAZGBC010000000
VERSION     DAZGBC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.239"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..126
                     /locus_tag="ACHK1J_004688"
     CDS             10..126
                     /locus_tag="ACHK1J_004688"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913855.1"
                     /translation="MSEKTFSTHKQNLIKRLRVHNEVELLYKGMLLVRKGRL"
     gene            201..389
                     /locus_tag="ACHK1J_004689"
     CDS             201..389
                     /locus_tag="ACHK1J_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO7913856.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            533..1378
                     /gene="mscS"
                     /locus_tag="ACHK1J_004690"
     CDS             533..1378
                     /gene="mscS"
                     /locus_tag="ACHK1J_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO7913857.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1504..2724
                     /locus_tag="ACHK1J_004691"
     CDS             1504..2724
                     /locus_tag="ACHK1J_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO7913858.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            2955..4700
                     /locus_tag="ACHK1J_004692"
     CDS             2955..4700
                     /locus_tag="ACHK1J_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7913859.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMAVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(4882..5511)
                     /locus_tag="ACHK1J_004693"
     CDS             complement(4882..5511)
                     /locus_tag="ACHK1J_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO7913860.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYAEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
BASE COUNT         1494 a         1107 c         1491 g         1833 t
ORIGIN      
        1 tatcttggca tgtcagagaa aacattctcc actcataagc agaatctgat aaagaggtta
       61 cgggtacata atgaagttga gttgttgtac aaggggatgc ttctggtcag aaaagggcgg
      121 ttgtgaagca gggcagctta tttccggtaa tgttaaacgc agcctctgat tcgttaattt
      181 cataatcata aggagttttt atgcgtctgt tgattgcttt tcttcttccc tggctgactt
      241 ttttcaccat cggccggcct ctgacgggga ttttttgtct ggtgttacag ataacgctgc
      301 tgggctggat accggcaaca atatgggccg tgtatgcgct ttcgcagtat aagacggaac
      361 agaaaatccg cgaagccacg gggaaatgat gactgcagaa gagttggttg tcataccggt
      421 gtatatgatg cgggataaag gcccggagat tcctggaaat aaaatgttcc ggggaatata
      481 aaacagggaa aaaccggatg tgatggacgc ttctggcgag gcggtttcat gaattgtccg
      541 taatcaggat atgttgcccg gttacgccgc gaatcttgtg gcagcagtgg ttattattat
      601 cgggggtatc gctgcacgga tgatttctgg tgtgctgaat aatctgatgc tgggcaggaa
      661 tattgatgcc acagtggcgc tgttcctttc cgcgctgata cgtcatgcca ttatagcctt
      721 tacgctgatt gctgccctgg gacgtgtggg ggttcagaca gcttccgtaa ttgcagtcct
      781 gggggctgca ggtcttgctg ttggacttgc cctgcagggg tcattgtcaa accttgcagc
      841 tggtattctt ctcgtgatgt tccgtccttt cagggctggt gaatatgctg acctgggtgg
      901 tgttgccggg actgtgcaga atgtacatat attctcaacc acgatgcgta cactggatgg
      961 taaaattgtt gtcattccga acgggaagat tattgccgga gagattgtta atttctccag
     1021 ggagcctgaa cgacgaaatg agtttatcat cagtgtttca tatgatgcag atattgactg
     1081 tgtgaaacaa gtcctgacag atattgtgat gtcagaagag agggttctga agaacaggga
     1141 aatcactgtt cgcctgaatg agcctgggga gtcatccatg aattttgtag tacgggtatg
     1201 gagcaggcgt gatgaccttc agtctgtata ctgggatgta ctggagagaa ttaagcggga
     1261 gtttgattca gaagggataa gttttccata tccacaaatg gatatccatc ttgttcgctc
     1321 cgggaagcag gcagagtcgg taacccataa cagtcgctca gtcagttcca gacagtaaaa
     1381 tgaaaagcgc attttatttg tgaaagatga acagcctgaa tctgtcaggc tgttttcatt
     1441 gtgtttttat ggtcattttt ggtatatgta ctgtatataa tgtcatctgt acaaggatat
     1501 aaaatgccaa agaaaataat gacagggatt gatgtttttg acgctgccat ggagcgtatt
     1561 gaatgggtgt ttgatacgtt ttcatcagtt tgtctttctt tttccggcgg aaaagactcc
     1621 actgtcctgt ttcacctgat ggcgaatatt gcccgccgaa aaaaacgccg tttttctgta
     1681 ctgtttgttg actgggaggc tcagtatcag tgtaccattg agcatgtcac caggttgagg
     1741 gaaatgtacg atgatatcac agatactttt ttctgggtgg cgctgcctct gacgaccgtt
     1801 aatggagtgt ctcaggtaca gcctgagtgg gtatgttggg aggacggggc tgaatgggtt
     1861 cgccagccac cagaagatgc cataaccaga gcgtcttttt ttccttttta tcagaatgcg
     1921 atgacatttg aagagtttat tccggcattt aatcattggt ttgcccggga aaaagggatg
     1981 gttgctgcga ttcttactgg tgttcgcgct gatgagtcac tgaatcgttt tatgggactg
     2041 gtatccggac gtaagctccg ttatgcggag gataaaccct ggaccacggc atcggctgag
     2101 ggattttact acaccatgta tcctttgtac gactggaaaa caagagatgt atggatttac
     2161 catgcgagaa cacgtgctgc ctataactct ctttatgacc tgatgtacag ggctggagtt
     2221 cctcttcgca atatgcgggt gtgtgagcct tttggaccgg agcaacgtaa ggggttgtgg
     2281 ctttatcatg tactggagcc ggatacatgg ggcagaatgt gtgctcgcgt atcaggtgct
     2341 gtagggggag cgctctatgc aaatgaaagt ggtgcttatt ttgctttgag gaaacagata
     2401 acaaaaccag ttcaccatac atggcagagt tatgcaatgt ttctgctgga gtcaatgcct
     2461 gagaaaacgg cggagcatta caagaataag attgctgttt atcttcggtg gtggcagtct
     2521 catggatggc cggatggcat ccctgatgag caggataatg accagggatg taaagatatt
     2581 ccctcctggc gtcgcatctg taagacgtta ctgaaaaatg atttctggtg tcggatgctg
     2641 gcgtttagtc cgaataagcc ccgacattat gagcgctacc tggccagaat gcgtgaaaaa
     2701 aggaaagagt ggggaatatt gtaaattata tttaaacatc agaaatatga cggttaatcc
     2761 aggatagtag ctaaaaaaat aagattcctt gctgatttat ccctgtttga tatgtaaaat
     2821 ctgaggggct tataagcaac ggatatacga gtgctacaat atatgataag ttatatatat
     2881 taaatgttca tcatctgtca gggggctgtt ttatggctaa actgataaat atatcaggtg
     2941 ttaccgagga gcggatgact gccggggaaa agcgggttgc atcccgtctt gaggcattta
     3001 tgagtaatga gtgtctggtc tggtacgata ttccggtggg acggaaaaac cgacatcctg
     3061 attttgtcat tattgacccg gagaatggtc tggtatttct tgaagtcaag gactggaccg
     3121 ttcacacgtt gcatcaggta aatcacgaac aggtcattct tgaaactaac ggtattctta
     3181 aatcagaaat aaatccgctg ctacaggttc gtcgttatgc ctgtgatacc gtaaatgctt
     3241 tacctgcaga ctcacgtttg cgacagaatg atggtcagta taaggggcgt ctgaatctgg
     3301 cctgggcgta tggtgttgtg tttacccgta ttacccgcca gcaactgaag gctctggctg
     3361 ggaatgatga aaatgctgtg gaaaggattt tcccttcagc gcagacaatc tgtcaggatg
     3421 atatgactca gtctgtttta cctgatatat tccgccaaaa aattgccggg atgtttacga
     3481 tgggattcag aacccgggtg acaccacagg ttagagatat tctgcgtgct catctgtttc
     3541 ctgaaatggc cgtcagacag cactcacaaa tcaaaataat ggatattcag caggagattc
     3601 tggccagaaa tattggtgat gggcatcgtg ttattcatgg ggtagccggc tctggaaaaa
     3661 ccatgattct tttgttccgt tgtctttacc tggctgaaac tacccctggt aagattctgg
     3721 tgctatgtta caacattact ctggccagct acctgcggga atgtattgag tccaggggac
     3781 tgaagtcacg ggttacggta ttccattttc atagttggtg cgcctcaatg ataaagcgcc
     3841 atggaattca ggtgactgct ggaggaaagg attacccgga gaaatgcttc agtgcgctgg
     3901 aagatgccgt gaacagcgga acaattactg atactggata cgatgccgta cttgtggatg
     3961 aggggcatga ttttgagagt cgctggctgg ctctgatagc ccgcctgttt gataacgcgt
     4021 cccgttcact gctcctgatg tacgatgacg cacagtccat ttacagacgg gaacgggctc
     4081 tgaacttttc gcttgccagt gtggggattc aggcgcaggg acgtacttcc gtcctgccgg
     4141 taaattaccg taatacgaaa cgcattctgc actttgctta tgcattctcc cgtgagtatt
     4201 ttgagaagca tcagaaccgt gaaataccgt ttgttcagcc acaggcgggc ggtgaagagg
     4261 gcacagagcc ggaaattctg cgttgtgctt ctgagagtga tgaggcagtg caggttgtgg
     4321 gctggctgga gaaacgctat acactgtgcg gtcactggag tgatatggct gttttgtgtc
     4381 ctgcagagtt ttcagtgaaa catctgaaag aggtcatgac acaacgcggg ataccttatg
     4441 ccacctgctt tgacagtgaa ggtaagaaga aatattcgcg acgcaaggat gttgtgcatt
     4501 tactgaccta ccagagcagt aagggactgg agtttccgta tgttgcggtt atcaatgctt
     4561 ctttcgttca ctcaggtgct gcggatgagt ctgaagttat tccggcgctt tatgttgcgt
     4621 tcacccgggc tacacgggaa ttactggtga cctgttatcg tgaaaacagt atcagtcgtc
     4681 atcttgagga ttttgtataa gttgtgtttt attattgctg atatgtaatc attcgttttt
     4741 tgataatcta agatgggtat ttagcgagta ttcagagttg aattaatatt ccccatgttt
     4801 ttgatctgtc aatagctgtc cattacatgg ggtgatcata tatcagtcgg taattactgg
     4861 ataaagtcac cccctaaatg atcactttat tgtccatgcc ggactaaagt tttcctcctc
     4921 aaatagttct gttaacccac ttatttgttt taatctcagt acctcatctg tatccatgcc
     4981 cagttcacta cctattcgga gatcatccca tcccatccgg gaaagatctc ggacgatatc
     5041 ggagagcgcg tctatctggt gctttcctct ggccctgtta tgcctgatgg tcgaggcgat
     5101 agagtaagct ttaaagttat tatcatgctt aatgcatgtg acaggtaaat atccttttag
     5161 ttttttctct agttttgttg tgcttttccc aagcatttgt ctgtgaaatc cgtcaataac
     5221 ggtgtatttc cccttttgag ctaaggtgac aacaggttga gtgaatccat caaccaacag
     5281 ggagtgtgtt aataaccttt tttcggaggg agccatgacg ttaggattat agtcgtttgc
     5341 ttcaattaga ttgtttttta tccatataac acaatcaaca ggttctgcgt aaaaaggact
     5401 actcctatgt aactcaatct ttattttatt aattgcatca atgcgttcat cttctttctt
     5461 aagagaatta aggtatacat ttatttcatt tatgatttgt tcaatgctca tgtgtttcta
     5521 tttcttttaa tttgtatatt tttcttgttt taagatgagt tgttcttctt tctctacttt
     5581 gtgttaatat ataagttgta aggttgtttt gtgtttttat ttttggtgct ggaggagata
     5641 atatgctcct catgataaac gatgtgattt tcaaataaat aatgctgggt ataatatttt
     5701 cttttgaata ttaaagagtt tatgaaaaga aagttttctg gctatgtctc cttattctga
     5761 tataacttca cgtatggaac catttagcat aaaaatattt aattttatat gaccaacagt
     5821 tcttgttaaa gaatattagc tagttttcat tatattataa acaaagagtt ctttgctgtg
     5881 atggatttca cattttattg tcttgttatg tattccttct ggttt
//
LOCUS       DAZGBC010000238         5900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGBC010000238 DAZGBC010000000
VERSION     DAZGBC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.240"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..4858)
                     /locus_tag="ACHK1J_004694"
                     /pseudo
     CDS             complement(1..4858)
                     /locus_tag="ACHK1J_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4878..5339)
                     /locus_tag="ACHK1J_004695"
     CDS             complement(4878..5339)
                     /locus_tag="ACHK1J_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO7913861.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5367..>5900)
                     /locus_tag="ACHK1J_004696"
     CDS             complement(5367..>5900)
                     /locus_tag="ACHK1J_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913862.1"
                     /translation="PGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKD
                     MDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLMVEKGDKITGVRKGSLSENVFQ
                     DRGTIAGSVHVDAVDNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVI
                     RLSKDGITIVGGSVFIN"
BASE COUNT         1073 a         1799 c         1558 g         1470 t
ORIGIN      
        1 tatcaatccc accaaacaca tctgaatata tatccactgc tgatggtttt gttttattaa
       61 gtgttatatc aaatccttca ataactggat gtaatccata ttggtttgta ggagatgtga
      121 tatcaataat accggcatct agacgtttgg aaccacctgt ataatgagtg ccggatgtcc
      181 acttaccatt tactttaaat ggtccattcg ttgtcgcagg aactcgctct ccatttttta
      241 tggctggttg aatctcaact ctaaatttac tacctgttaa ttctagtctt ttatttatca
      301 tgtttatgta tttattgaat gcagcctctg attttatcca atcaccatta gcatatttaa
      361 gtgctctatt ccctaatgag ttcaaaacat cagatatgtt gctttgctca ctcccccatt
      421 tcgtccagcg ggctaactga catatagcca gccccaaagg atcgatatac ccacaaggat
      481 tcggcacata ccggtaagtc tgctcccctc cctgcaaccc cagcggatca ctcaccaggt
      541 acatcccggt ttccggctcg tagtaccgga accggttata gcacagcccc gattctgcat
      601 cctgccactg tcccgcatac agcaggccgg ggtccgcttc cgggtcccgc tccccgcgcg
      661 ggtccgggta gccggtgtct gcgggcaggc tgagtgccag cccccacagg ctggtctgcc
      721 ccggccgcca gacggtttta ccttcactgt taaagagcat cagcgggcgg cccgtcaggt
      781 cgctcaccgc atgattcgtt cgcgtcaccc actgcggggc cacggacggg tgcggctgcc
      841 gtacccggct gcactgctgg cttatcagct caaagccgtt aaacaccagg tgccgtacgc
      901 tgtacagttc atcatcgcgg tattcccgga tttccgcaat actgtcgcca tcccacaggt
      961 acgtgaagcg gattttcttc ctgtcacagc gtttctctgt tcgtctgccg ctggcgtcgt
     1021 ggcggtattc ccactgttca ccctgtgcgc tgcaataccc ggtcagctgg tcccggctgt
     1081 cccaccggaa ccgctctgtt tccgggcggt agccgtcacg gtgttttgtg cggctgacca
     1141 tccggcctgc ggcgtcatag tcatactgcg tgtttccggc ctgtttcagc cggtggcctc
     1201 cggcatactg gtcagtctct tcacttatcc ggtgcctgcc cgcagactgc gccttcaggt
     1261 agccacaact gtcatagcca taggactcat cgccttcgcc atagcccagc ccggtgcctg
     1321 aggctgtcac cgccgtgacc tgaccgttcc ggtccagccg gtactcccgc cgtgtttccc
     1381 gtccgtaatc ttcccggctg gtgacagccg acacattgcc cgcgctgtcg taacggtact
     1441 cccgtaccag tgaggggagc agcccgcccc atgtggtttc ctccgggaaa aatccggcgc
     1501 gctgggtgac aagctggctg gctgcattcc agccctgctc acaggcaaaa ccccggttac
     1561 tgtgccggag cacttcacgt cccgcctcat cacggctgaa cgtcagcgtc tcatcccccg
     1621 gcagaatgac ctccgtcagt tctccggcgg cgttataacg gtacgtggtt ttcagcaggg
     1681 attcatcggg gtgtctgtcg cgttcatctt ccggtggcag gatacagcgg atgacggtgt
     1741 gttcgtcggg gtagtggtat tcaatggccc gtcggccctg agtggtgaca ttcacccgcc
     1801 cgcgttcatc atagccggtg cgctgtgatg tgccgtccgg gctgaagata cccgtcagcc
     1861 tgccggattt gtcatacgca tactgcgttt tgccttccgg cgtgatgatt tctgccaggc
     1921 ggcctgcggc gtcccggctg tagaggatag tttcctccag gccgttaacc ctggcggtac
     1981 acaggccatc ggcgtcatag cggtaacgac agaccacgcc gccccagtca cgttcttccg
     2041 tcacccggcc acagttgtcc agaatataaa gataagactc gccctgcggg ttaatgactt
     2101 ctgtcagttg cagcgtgtct ttatcgtagc ggtactgcca gcggtggcct tcaccgtccg
     2161 ttcgcgccac cggcaaatca aacgggccgt attccattgt ccagacaacg ccgttaccgt
     2221 ccgtgtacgc cagcaggttg ccgtggcggt catagcggaa tccttctgcg ccggttcctg
     2281 cccgctcaag gccgctcagt ttatggtgct gctgccactg caaccgcact gactaaccgc
     2341 cggggccgcg catcttcacc ggcaggtcgt gaccactgta ttcggtgtgg tgttcacggc
     2401 ccatcacgtc ggtgagtgtt tccggccggt tgtggtggtc gtactctatg cccaggtgcc
     2461 gttcatcctg gcggaatatt cccgacagca gcccctgtgc ggtgtatgcc agccgggtct
     2521 cttcgccctg tggcgacacc agactcaccg gcaggccgcg ttcatcacac tgctgcgtcc
     2581 acgtctgccc ggcagggtcg gtgatgttgc tgagcaggcc gcgctcatca tagcagtact
     2641 gccactccgc gccgtctgcc gccgtataac agcttaactg accatgtgcg gcgttgtatt
     2701 caaatttctc cacccgcccg gcaggggagt ggcgggccag caggtgatgg tattcatccc
     2761 attcatacgt ggtggtgctg ccgtccggcg cttcttcccg cagaatattg ccgtcaggat
     2821 catagtaata acggtgagcg cccttgccgt ccgtcacggt ggtgcagccg tccccatagt
     2881 ccagcgtggc gttgtaatag ccctccgccc cggtgacggt cacgcaccgg ccctgttcat
     2941 cgtaggtgaa acggctccac gtctggtcgt tatcggacca gcggatgatc cggtccgcag
     3001 cgtcgtactc ataaaacagg tggtagtcca gccgcgcatc cgcttctgtc agccgccccc
     3061 gcgcatcctg ttcgtaggtc gccagcgtct gtatgccgtt atccgtgcgg tgaatggctt
     3121 tcaggtagcc gctggcgtgg tacgtcagtt taagccggat cccgtcggtg tgactgacac
     3181 ccgtgatccg gtactggctg tcgcgctcaa acgtgatgcg gttgctggcg gtgtcgctca
     3241 tcccgcccag catgtacatc cggtctgcgg ggctgtgtgc caggcggtca tcgctggtat
     3301 cgtcagtcac cgggtggcgc agacgcattc ccggaaaggc gtcataaaag aagcggctgc
     3361 tcagtgcatc cctgtcgaac agctccagat aatcatcacg gcggcgcaga atgtactggg
     3421 cgtgatacgg gttaaccgcc gtatcacttt caccgtcgaa ggcgaagttc agcgtggctc
     3481 ccaggctgat gatatccacc cggtttccct gttcacgcac ccaggcgtat tcgctccagg
     3541 agtcactcca gcccacgccc gtcaggccat gcagtggcag taccgaactg tggaaacggg
     3601 tgaaggagag ggggagggtc tggcccaggg tgaagtcggt acgttcgtcg cagtacgcgc
     3661 cggttaccgg atcgacagga tctgttaccc atctgatgac tttattggca atacttttta
     3721 acgacttacc tgttttcccc agcaacgcgc cgagttttgc gcccagcagc tttccgcttt
     3781 tccccagcag gtttcccagt ttgcccagtt tcgccagatt ccccagtttt cccagcagtc
     3841 ctgctggcgg cagaagatag ctggctatcg ttattcctcc caggatcatt ctctgccatg
     3901 gcgggaactc cggctggata tccagatagg tgacctgctc acccccaata aagacgtccg
     3961 gcgaaccttc cacgatcgcc gcactgcact ccagtttgtc cccctggcgc gcggcaggct
     4021 gattgttgat aaagacgttg ctgctgccct gggctatcct cggtggtttg ttctccttgt
     4081 cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac atcttttttg atttcgccac
     4141 gcccgcccac catcgtggca aattccatta ataccgggcc gctgggaaac agaagcgatg
     4201 cgcctgcact gacgcgatcc ataaaactgg cattgctgtc cgtcagggtg ataacagcgt
     4261 tcccccaggc cttcgccagt ggtactgctt tttccgctgc cgattcaagc aggtcagtgg
     4321 caatatcacc ccatgagggt tccccctgtg ggacttcagg tattgctcca ggtgggggag
     4381 gagggggaag ggtgacggct gcacgggctg ctggctttcc gttaattttt acgttttcag
     4441 aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt tatccatgat tcaagctggt
     4501 tctgtttctc ctccacataa tcactcgcct tatagctggc atacatcagc gcaggcacca
     4561 gaaccagcgc ataagggccg gtggcacagg cggccataat caggcaacct tctgcgatgg
     4621 tcactatcgc ccccagcacg gcccctgcca gcgcccccaa aaaactcttg tgctgaattt
     4681 catcgcccag cctggcggcg ggggagccag acttttttga tgccaacatc tgtgcaatgg
     4741 ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat cgcctgctgt gacagcattc
     4801 gcattgccag cgtaccgcct gcggtcgctc cctgtggccc gcctggtcct tcactcatgt
     4861 gttatccctc gtttatgtca gcgcggctgg tccttttgga tgtcattctc cggtggcaac
     4921 ggggtgaagc tctttatcac ctccagcagc atcgtctttt gactgttctt cagggataag
     4981 gaggtgctga aagtgaaggt cagcagcgta tctccacgcc gctggaatac ggctatctgg
     5041 ctaatccgcc cctgattccc ttcatcttcc ggaccgttcc aggaataatc cagcagccag
     5101 gccggggcct ctgccagagt gatttcccgg caggcatgtt cggtgtaccc atcaagtttg
     5161 ccctggtaca gcgccagcgt ttcctggtac agctctggtg tcagggttcc ctgtggaacg
     5221 gcttcgcggg taatgaccag gttgatgcca ctgttatctg tggacaccag gacctgcatt
     5281 gactgatcct gccattgaga cggcagttga atgtggcctt cattaaaagt gaactgcata
     5341 aaatactccc tgataatctg agtgaatcag ttgataaaaa cagaaccgcc cacgatggtg
     5401 atgccatcct tcgacagcct gattaccgtt gattccccga cctgtagctg aatgccatcc
     5461 ggcgtcagcg caattttgct ttcctccacg gccagcataa tttccttaat tgccgtataa
     5521 gcctgtatac cgttgccttc gccaccattg tctacagcgt caacatgcac cgaaccggca
     5581 atcgtgcctc tgtcctgaaa gacattctca gataaagaac cttttcgtac tcctgtgatc
     5641 ttgtcgcctt tttccaccat aagaaggcga ttaccttcct gtatcgtgac cgtctggtca
     5701 ttttttacgg tttcgcgatg atcgtgtttt acggtggtgg tacgatcgtt gagtacctcg
     5761 gtatccatgt ccttctgcgc gtggatgtag acctgttctt tccctgtcgc atcgtcaaac
     5821 ttcagctcat taaacccgct gcccatatac gttttggaac ggatggtcat ctgcgtcttc
     5881 gtccccggca ggctgccggg
//
LOCUS       DAZGBC010000239         5891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGBC010000239 DAZGBC010000000
VERSION     DAZGBC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.241"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..796
                     /gene="fucA"
                     /locus_tag="ACHK1J_004697"
     CDS             149..796
                     /gene="fucA"
                     /locus_tag="ACHK1J_004697"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO7913863.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDTVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            824..1972
                     /gene="fucO"
                     /locus_tag="ACHK1J_004698"
     CDS             824..1972
                     /gene="fucO"
                     /locus_tag="ACHK1J_004698"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO7913864.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIICNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2027..2782)
                     /gene="xni"
                     /locus_tag="ACHK1J_004699"
     CDS             complement(2027..2782)
                     /gene="xni"
                     /locus_tag="ACHK1J_004699"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO7913865.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMLALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2894..4261)
                     /gene="sdaB"
                     /locus_tag="ACHK1J_004700"
     CDS             complement(2894..4261)
                     /gene="sdaB"
                     /locus_tag="ACHK1J_004700"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO7913866.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4319..5608)
                     /gene="sdaC"
                     /locus_tag="ACHK1J_004701"
     CDS             complement(4319..5608)
                     /gene="sdaC"
                     /locus_tag="ACHK1J_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO7913867.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1472 a         1469 c         1579 g         1371 t
ORIGIN      
        1 gtgattagtt gagccaggtc acaaaatata agcttgtgaa tttctttctt gttaatcagg
       61 aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca acattattgc
      121 cctgttttga atcagagaga ggtagctaat ggaacgaaat aaacttgctc gtcagattat
      181 tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg ggaacgtcag
      241 tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg aaaaactgac
      301 ggagtcgcat attgttttta ttgatggcaa cggtaaacat gaggaaggaa agctcccctc
      361 aagcgagtgg cgtttccata tggcggccta tcaaagcaga ccggatgcca acgcggttgt
      421 tcacaatcat gccgttcatt gcacagcagt ttccattctt aaccgaccga tccccgctat
      481 tcactacatg attgcggcgg ctggcggtaa ttctattcct tgcgcgcctt atgcgacctt
      541 tggaacacgc gaactttctg aacatgttgc gctggctctc aaaaatcgta aggcaacttt
      601 gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt tatggctggc
      661 gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta cggacacggt
      721 gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa cctatgggtt
      781 acggattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta acagaatgat
      841 tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg atgaggtgaa
      901 acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc aatgcggcgt
      961 ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga tttacgacgg
     1021 cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat tccagaatag
     1081 cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt gtaaagcgat
     1141 tggcattatc tgcaacaacc cggagtttgc cgatgtgcgt agcctggaag gactttcccc
     1201 gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggca ctgcggcaga
     1261 agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg tttgcgttga
     1321 tccgcatgat atcccgcagg tggcgtttat tgacgctgac atgatggatg gtatgcctcc
     1381 agcgctgaaa gctgcgacgg gtgtcgatgc gctcactcat gctattgagg ggtatattac
     1441 ccgtggcgcg tgggcgctaa ccgatgcact gcacattaaa gcgattgaaa tcattgctgg
     1501 ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg cgctcgggca
     1561 gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg ttggtgcatg gtatggcgca
     1621 tccactgggc gcgttttaca acactccaca cggtgttgcg aacgccatcc tgttaccgca
     1681 tgtcatgcgc tataacgctg actttaccgg tgagaagtac cgcgatatcg cgcgcgttat
     1741 gggcgtgaaa gtggaaggta tgagtctgga agaggcgcgt aatgccgctg ttgaagcggt
     1801 gtttgctctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg gtgtacgcaa
     1861 ggaagacatt ccggcactgg ctcaggcggc actggatgat gtttgtaccg gtggcaaccc
     1921 gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt aaatgcgctg
     1981 atgtgataat gccggatacg acgtttgcgg cgtatccggc tcgccgttac cgtaccaatc
     2041 gcaattgctg taaattgcca tcgatatgca aatcggtttg taagcgggca atatcgcggc
     2101 acagaaacgc catctcttta tgggtttcta attttttgcg ccacttttcg gcaaccgcat
     2161 ccagattctc atatatccct tccagactct gaaactcgac cagcagctgt gtggcgcttt
     2221 ttggtccgat tcccgcaaca cccggtacct ttgaactgct gatccccgca agtccccagt
     2281 aatcgggcaa ctgctgcggt tgaacgccaa attctttatc gataaatggc gcatccagcc
     2341 aacgtttctg gaagtaatca cgaatacgta atgtcggtga aagtaactga cagtagcctt
     2401 tatctgtcga aacaatcgtt gcctgatgcc cggcctgtgt cactttgacc gccagcgtgg
     2461 cggctaagtc atcggcttcg ttgccgctgg ttgaccagca cgggacgccg cgttgctcaa
     2521 aggcggcgcg taatgcaagc atctcgtcgt gcaactcttc cggcattggc ggtcgacccg
     2581 ctttgtaatc tggtaaacgc tgatgacgcc agccgctact gcggttttca tcatcaaaaa
     2641 cggcgacagc gtgggttggc tggctgtgca taatgagctg atcgagcgcg tgctggcagg
     2701 tttcgacaca gggcgaaccc tgaacggcat gaatgcgacg aataagattc agtgcatcga
     2761 caataagcaa atgaacagcc acgataaccc ccttaaagat caggcgataa gggtaacatt
     2821 cgatactgga acaggctatg gctggatgag aaatcgggaa gaggcctcgc aaaacgaggc
     2881 ctctggagag cgattaatcg caggcaacga tcttcattgc caggccgccg cgagaggttt
     2941 cgcggtactt ggcgttcata tctttacctg tttcgtacat ggtttcgata actttatcga
     3001 ggcagacgcg cggctcgctg gtacggcgca gcgccatacg tgcggcgttc accgctttta
     3061 ctgccgcaat ggcgttacgc tcgatgcatg gcacctgtac ctgtccggcg accgggtcac
     3121 acgtcagacc gaggttgtgc tccatggcga tttccgccgc gatgcacacc tgcgccgggc
     3181 ttgcgcctaa tagttctgcc agaccagccg ccgccattga gcacgccacg ccaacttcac
     3241 cctggcaacc cacttcagca ccagaaatcg acgcgttcat cttataaaga gaaccaatgg
     3301 cgctggctac cagcaggtaa cgagccagtg agttagcgtt cacttcgcgg ataaacttat
     3361 cgtagtacgc cagcacagcc gggataatcc cgcacgcacc gttagtcggc gcagtcacca
     3421 cgcgaccgcc agcggcgttc tcttcgttca ctgccagtgc aaacatgttg atccagtcaa
     3481 caaccgccat cgggtcagtg gtggttttat cctggctgac cagcatccgg cgtagtgccg
     3541 cagcacggcg tggaacgcgc agtttgccag gcaacacgcc ttcggtggaa ataccgcgct
     3601 caataccgcc gcgcatgact tcccagacgt tcgccaggtg ctgttccagc tcttctttgc
     3661 tgtgcagcgc cagctcgttt ttcatcatca ggccagagag tgacagcccg gtttcctgac
     3721 aatgtttttg cagatcggct gctgaactgt acggataagg aacttcaacc ggtgcgctat
     3781 cctgctggcc aaaatgctct tcatcaacga taaagccacc gccaatagag tagtaagtct
     3841 gactgtaaac gactttatca cccgccagcg cggtaatgcg cataccgttt tcatgcagag
     3901 aaaggttgtc ggcatggaag ttcatgcact gatcaaccgg gaactccact tcatgctgac
     3961 cgtttgccag catcaggcga ccatgagtat tcacatcctg aataaaacca gggatggaat
     4021 cgatatccac ggtatccggc aggttacccg ccagacccat aattatggcg atatcagtgt
     4081 ggtggccttt accggtcaga gagagcgagc cgtacacgtc aaccaccacg cgggtcacgt
     4141 ctttaagcag gttacgggca atcagatcgt cggtaaattg tttacccgct ttcattggtc
     4201 caacggtatg agaactggaa gggccaatgc cgattttgaa aatatcgaat acgctaatca
     4261 taggaaatac atcgcgttaa aacggaggaa gcgccgcccg aaagcggcgc gaaaggactt
     4321 agctgaacag agagtagaag attgcggaga ttgcaatcag acccatcacg acaacgaata
     4381 cgttgctgat gtgaccgctg tacttacgca ttgccggtac tttctgaatt gcgtacatcg
     4441 gcatcaggaa caggatcatc gcgatgattg ggccgcccag ggtttcaatc atacccagga
     4501 tgctcgggtt cagggtggca acaatccagg tcgttaccag catgaacagc gcagtgatac
     4561 ggttcagctt gttgatttcg atagacttac ctttaccacg cagagattta atcaccatac
     4621 cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa ggatttggtg atagcgataa
     4681 tcgcgataat cggagccatc cacgcgataa ccggtgcgtt aaagtggtta gccaggtaag
     4741 acagaatcga gatgttctgc tctttagccg cagccaggtc tgccggagtc aggctcagta
     4801 cacagctgaa gacgaagaac attacggtca gcaccatcat gatgtgtgcg aatgccagga
     4861 tcttcgagca tttctgttct gccatatcgc cgtactcttc acgcttcgca acggcgaaag
     4921 aagagatgat cggagagtgg ttgaacgaga acaccattac cggaattgcc agccacaggg
     4981 tcatccacag accgtttccg gttgcagatg cagtgtccag agacagcgtt tccagtgctg
     5041 cgccgttcca ctgcgggatc aggtacagag ccagcagcat caatacgcca acaaacggga
     5101 acaccagaat actcatcgct ttaacgatca tctgctcacc gaagcgaacg atggtcatca
     5161 tacccacgat caggatcagc gacagaatcg cacgcggcgg tggcgtcata cccagctggt
     5221 gagacatgaa gctttcaact gtattggtga ttgccacgct ataaaccagc aggatcgggt
     5281 aaatagcgaa gaagtagagc agggtaatca gtttacctgc gccaatacca aagtgttctt
     5341 ctacaacctc ggtgatgtct tcgcccgggt ttttaccaga cagtacgaag cgagtcaggc
     5401 cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc catgatgatc agcgggatca
     5461 taccgccaac accggcgttg attggcagga acagcacgcc cgcgccgatt gccgtgccgt
     5521 aaaggcccag catccacatg gtgtctgtct tgcgccaggc actacgagag tctttcgacg
     5581 caatcgtgct ggtttgagtc gtttccatct atttctcctg gaggaatgca aataattcag
     5641 cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa aatgaaatct gtctatcggc
     5701 gatggcttaa tcatttaccg ccgtaataat ttgagggcgg aaagatacac acaagttatg
     5761 tatctatcag tgatcttgat cccaaataca ataatctact tggaagatag attatcccaa
     5821 cgctttactc tgtaaatcaa aagtaaaaga gaatttaagg gcaatatttg cccattaaac
     5881 gacatcttac t
//
LOCUS       DAZGBC010000240         5868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGBC010000240 DAZGBC010000000
VERSION     DAZGBC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.242"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..76
                     /locus_tag="ACHK1J_004702"
     CDS             <1..76
                     /locus_tag="ACHK1J_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913868.1"
                     /translation="NTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(150..1352)
                     /gene="ubiI"
                     /locus_tag="ACHK1J_004703"
                     /gene_synonym="visC"
     CDS             complement(150..1352)
                     /gene="ubiI"
                     /locus_tag="ACHK1J_004703"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO7913869.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1375..2553)
                     /gene="ubiH"
                     /locus_tag="ACHK1J_004704"
                     /gene_synonym="visB"
     CDS             complement(1375..2553)
                     /gene="ubiH"
                     /locus_tag="ACHK1J_004704"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO7913870.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLECGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2550..3875)
                     /gene="pepP"
                     /locus_tag="ACHK1J_004705"
     CDS             complement(2550..3875)
                     /gene="pepP"
                     /locus_tag="ACHK1J_004705"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO7913871.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3901..4479)
                     /gene="ygfB"
                     /locus_tag="ACHK1J_004706"
     CDS             complement(3901..4479)
                     /gene="ygfB"
                     /locus_tag="ACHK1J_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO7913872.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4647..4976
                     /gene="zapA"
                     /locus_tag="ACHK1J_004707"
     CDS             4647..4976
                     /gene="zapA"
                     /locus_tag="ACHK1J_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7913873.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            5018..5201
                     /gene="ssrS"
                     /locus_tag="ACHK1J_004708"
     ncRNA           5018..5201
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK1J_004708"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5222..5824
                     /gene="fau"
                     /locus_tag="ACHK1J_004709"
     CDS             5222..5824
                     /gene="fau"
                     /locus_tag="ACHK1J_004709"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO7913874.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
BASE COUNT         1395 a         1643 c         1508 g         1322 t
ORIGIN      
        1 aaacactaaa tcaaaaaatc atggcattag aaaatataat gcgaaaacgg aggtgaaatt
       61 agtttatttc aaatgaggaa aatctcccgg ggaaaaaacc gggagatgaa agtgtgatgg
      121 gtatcaaata aacaacagag gagaaatttt taacgcagcc attcaggcaa atcgtttaat
      181 cccattgcct ggcggataag ttgcggctta acgccaggaa gcgtgtcggc cagtttcaaa
      241 ccaatatcac gcagcagttt tttcgccgga ttagcgccgg aaaagagatc gcgaaaaccc
      301 tgcataccag ccagcatcag cgccgcgctg tgcttgcggc tacgttcata gcggcgcagg
      361 taaatgtact gcccgatgtc tttaccctga cgatgcaacc gtttcagttc agcaatcagc
      421 tctgcagcat ccataaagcc gagatttacc ccctgccccg ccagcgggtg aatggtatgc
      481 gcggcgtcgc ccaccagcgc cagacgatgc gcggcaaact ggcgcgcata acgccccgtc
      541 agtgggaaca cctgacgcgc gctctcaacc ttgcataagc caaggcgatt atcaaaagcg
      601 atatttaacg cgcgattaaa ttcgtcttca cttgcctgct gcatccgctg cgcttcctct
      661 ggcgacagtg accagacaat cgagcaaaga tgcggatcgc taagcggtaa aaaggccaga
      721 atgccttcgc catggaaaac ctgccgcgcc accgcatcat gcggttcttc cgtgcgtatg
      781 gtcgctacca gcgcgtgatg ctgataatcc cagaaagtca gcggaatatc ggctttgttg
      841 cgcaaccagg aattagcgcc gtccgcgcca atcaccagac gcgccgttaa catactgcca
      901 tctttcagcg tcaggaaggt ttcgttttct ccccaggcga cctgctgtaa ttctgcgggg
      961 gccaacagag tgatatctga cgactgctgc gctttgttcc acagcgcgta gtgaatcact
     1021 gagttttcaa cgatatgccc aagatggcta tagcccatgc tttgatcgtc aaacgaaatg
     1081 tgaccaaaac tgtctttgtc ccacacttcc ataccgtgat agcaactggc cctacgagag
     1141 agaatgtctt gccagacgcc aagacgggtg agtaattttt cgctggcggc attgatagcc
     1201 gaaacgcgca gttgtggtgg tgcatccacc gccagaggtt ccggtacgcg ctgctccagt
     1261 acggcaacgc gtaagccgct cccctgtaag ccgcaggcaa ccgccagccc caccatgccg
     1321 ccgccaacaa tggctacatc aacactttgc attgtttatt ccttaaaacc gccttcaacg
     1381 cgccacccaa ccgagggtgc gctgcgccag cacatcgcgt gccggggtga ataattccat
     1441 cgtcatcagc ccgatgttgc gcccgacaac cagtggcgtc caacggttgg caaaaagatg
     1501 tacaaggctg tccgtgacgc caatggttgc ttcgcgatcg ctctgtcgac gctgctgata
     1561 acgacacaat acgccgtaat cacccatgtc ttctccgcgc tcctgcgcct gagtcagggt
     1621 ttccgcaaga ctcatgacat ctcgcatacc gaggttgaac ccttgcccgg caatcgggtg
     1681 cagggtttgc gccgcattgc ccaccagcac ggtacgatgg gtaatagatc tggcggcgtg
     1741 ggttaacgcc agcggataag cactgcgttt accagcgtgg gtaattttcc caagtcgcca
     1801 gccaaaggcc gactggagtt cacggcaaaa cttctcgtcg ctccacgaca gcacctcttc
     1861 gcgccgttcc agtggatgac accagaccag cgaacagcgt ccgtcagaca tcggcaacat
     1921 cgccagcggg ccatgttgcg taaagcgttc aaaagcgcgc ccttcatgcg caacggaagt
     1981 cgcaacgttg gcaatcacag ccagttgttc gtaaggctcc tgctgccagt caacgccgca
     2041 cacagtggct aacgctgaat gggtgccatc agcggctacc agcacgcggc ccgtcagcgt
     2101 ctcgccacac tccagcgtca cttcaacatg actctgagta cgggcaacgt tagccacgcg
     2161 atccgggcaa tgcagcgtta cgccaggtgc tttgcgcagc aatgcaaaca gccgttgccc
     2221 gacattgtgc aattcgacaa cctgccccag cgccgccagt tggtaatctt ctgcggcgag
     2281 ggtgacgaat ccggcatgac cacgatcgct gacatgcacg gtagtgatgg cggttgcgca
     2341 atccgccaga gattgccaga cgccaatgcg cgccagttgc tgacaggtac ccgccgccag
     2401 cgcaatcgct cgcccatcaa agcccggatg agcatgtgac tctggcgcgg tcgcttcaat
     2461 caaatgtacc ggcagcgccc cgtgacttaa ccgggaaata gccagcgcca gcgtcgcgcc
     2521 cgccatgccg ccaccgacga tgattacgct cattgctttc tcgcagctgc catcaacgct
     2581 tcgatttctt ccggcttttt caccacgctg gcggtgaggt tttcgttacc ggtttcggta
     2641 atcacaatgt cgtcttcaat acgaatgccg ataccgcgat attgttctgg cacttctgca
     2701 tccggcgcaa tatacagccc tggctctacg gtcagtacca tgcccggttc cagaatgcgc
     2761 gagcgatcct ggccataaac gccaacgtca tggacatcca gtcctaacca gtggctaagg
     2821 ccatgcataa agaaaggacg atgggcgttc tgagcgatca gttcatcaac atcacctttc
     2881 aggatgccga gttttaccag gccgctaacc atgatgcgca ccacttcacc ggtgacttcc
     2941 tgaatggaag ttcccggacg atacaggcgc aggctggttt cgagagactc aagcacaatg
     3001 tcgtagattt cacgctgagc ctgggtgaat ttgccgttga ccgggaaggt gcgggtaata
     3061 tcgccagcgt aacctttgta ttcacaaccc gcatcaatca acaccagatc tccgtcacgc
     3121 agttcacact cgttttcggt gtaatgcaga atgcagccgt tttcaccgct gccgacaatg
     3181 gtgttatagg aaggatagcg cgcgccgtgg cggttaaatt cgtggtgaat ttcgccttcc
     3241 aggtggtact cgaacattcc cggacggcat ttttccatcg cccgggtatg cgccagggcg
     3301 gtgatttcac ccgcgcggcg aagtacggca atctcttccg gcgatttgaa caggcgcatt
     3361 tcatgaacaa tgggacgcca gtcgatcatc gttgccggcg cggtgagatt ttgccgcgaa
     3421 cctttacgca gtttctcaag cgcgctgttc acgatttcat cagcatatgc atattcgccc
     3481 tgggcgtggt aaaccacatc cagaccatta agtagttgat aaagttgctg attaatttcg
     3541 ctgaatgcca gtgcgcggtc aacgcccagt ttctctggcg cggcatcctg acctaaacga
     3601 cggccaaacc agatctccgc cgtcaggtcg cgaacgcggt taaacagaac gctgtggtta
     3661 tgagtgtcat cgcttttaat cagcaccagc accgcttccg gttcgttaaa gccggtgaag
     3721 taccagaagt cactgttctg acgatagggg tattcgctgt cggcgctacg tgttacttct
     3781 ggtgcagcaa aaatcagcgc ggcgctgccg ggttgcattt gctccaccag ggcctgacgg
     3841 cgacgctgaa actcttgccg ggatatctca ctcataacac tctccttacg ttttttgttt
     3901 ttagtgtaga gtcggttttt gtacttctgg cgcggtcggt tgcggatgag taaaggtgtc
     3961 gtggcataac agcgcggcaa cacgaacgta ttcgatgatc tcttcaagcg acatttcaag
     4021 ctcttcctga tcttcgtctt cgtcgtagcc caactgcgcg atgttacgca gatcgtcgat
     4081 ggcttcaccg gtttcgccgg tcactttgtc cagcttcggt tgcgtaacgc caagaccaag
     4141 caggaagtga ttgacccaac cggccagcgc atcagcccga tcgaaaacgc tgacatcatc
     4201 gccatcaggc agataaagct gaaaaaggaa gccgtcatcc tgcagggcat cgctggtggc
     4261 agagtgcatt ttacgcagtg cctgtgccag ctcatgaccg aaagccatgc cttcgttcgt
     4321 caggtcgtga agtagcggca gccatgagct gtcatcgtta ccgccacata tcatcccact
     4381 gattaaacca tgcatctcag caggggtcag acccgtccct tgttggttca gatactggtt
     4441 catttcgttg taaccaggca tttcgttctg tatagacata agcattcgtc gtcaaaggga
     4501 ggaatattca tgatatgcta ccactttggg ccctggtgga ccagaaaagg gcttgtctct
     4561 tctcatcagg gtagctatag tgtcgcccct tcgcagacca tgggtctaaa gacgaaggca
     4621 gcgcagtcaa tcagcaggaa ggtggcatgt ctgcacaacc cgtcgatatc caaatttttg
     4681 gccgttcact gcgtgtgaac tgcccgcctg accaaaggga tgcgttgaat caggcagcgg
     4741 acgatctgaa ccaacggttg caagatctga aagaacgcac tagagtcaca aatactgaac
     4801 agttggtctt cattgccgca ttgaatatca gctatgagtt agcgcaagaa aaagcaaaga
     4861 ctcgtgacta cgcggcaagt atggaacagc gtattcggat gctgcagcag accatagaac
     4921 aagcgttact tgaacaaggt cgcatcaccg aaaaaactaa ccaaaacttt gaatgacact
     4981 tttcggttta ctgtggtaga gtaaccgtga agacaaaatt tctctgagat gttcgcaagc
     5041 gggccagtcc cctgagccga tatttcatac cacaagaatg tggcgctccg cggttggtga
     5101 gcatgctcgg tccgtccgag aagccttaaa actgcgacga cacattcacc ttgaaccaag
     5161 ggttcaaggg ttacagcctg cggcggcatc tcggagattc ccttcttatc tggcaccagc
     5221 catgacgcaa ctaccagaac tcccactgac attatcccga caagaaatcc gcaaaatgat
     5281 tcggcaacgt cgtcgtacgt taacgccgga acaacagcag gaaatgggtc aacaagccgc
     5341 tacccggatg atgacttatc ccccggtggt gatggcacat acggtcgctg tattcctctc
     5401 ttttgatggc gaactcgaca cccagccgct tatagaacaa ctctggcgcg ccggtaagcg
     5461 cgtatatctt ccagttttgc atccctttag tgccggtaat ttgctgttcc tgaattacca
     5521 tccgcaaagt gaactggtga tgaacaggtt gaagatccat gagccaaaac tggatgtgcg
     5581 tgacgtgcta cccctttccc gattagacgt gttgatcaca ccgctggtcg cctttgatga
     5641 gtacggtcag cgcctgggaa tgggcggtgg tttttatgat cggaccttac aaaactggca
     5701 gcactataaa atgcaaccgg tgggttatgc gcatgattgt cagttggtgg aaaaactccc
     5761 cgttgaagag tgggatatcc ctcttcctgc ggtggttaca ccgtcgaaag tctgggagtg
     5821 gtgagggcga cacacctgca tcgccctgat tgacatcgtt gattcttt
//
LOCUS       DAZGBC010000241         5840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGBC010000241 DAZGBC010000000
VERSION     DAZGBC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.243"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..323
                     /locus_tag="ACHK1J_004710"
     CDS             <1..323
                     /locus_tag="ACHK1J_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7913875.1"
                     /translation="SKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLKAAVRKEFVDD
                     NRVKVNSDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLGVGYSNGAGVESPWNAVAGV
                     NWSF"
     gene            complement(695..961)
                     /gene="minE"
                     /locus_tag="ACHK1J_004711"
     CDS             complement(695..961)
                     /gene="minE"
                     /locus_tag="ACHK1J_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO7913876.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(965..1777)
                     /gene="minD"
                     /locus_tag="ACHK1J_004712"
     CDS             complement(965..1777)
                     /gene="minD"
                     /locus_tag="ACHK1J_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO7913877.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1801..2496)
                     /gene="minC"
                     /locus_tag="ACHK1J_004713"
     CDS             complement(1801..2496)
                     /gene="minC"
                     /locus_tag="ACHK1J_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO7913878.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3016..3384
                     /locus_tag="ACHK1J_004714"
     CDS             3016..3384
                     /locus_tag="ACHK1J_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO7913879.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3504..3905)
                     /gene="pliG"
                     /locus_tag="ACHK1J_004715"
     CDS             complement(3504..3905)
                     /gene="pliG"
                     /locus_tag="ACHK1J_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO7913880.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            3976..4134
                     /locus_tag="ACHK1J_004716"
     CDS             3976..4134
                     /locus_tag="ACHK1J_004716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913881.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4146..4439
                     /locus_tag="ACHK1J_004717"
     CDS             4146..4439
                     /locus_tag="ACHK1J_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO7913882.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4511..5170
                     /locus_tag="ACHK1J_004718"
     CDS             4511..5170
                     /locus_tag="ACHK1J_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO7913883.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5262..5708
                     /locus_tag="ACHK1J_004719"
     CDS             5262..5708
                     /locus_tag="ACHK1J_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO7913884.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         1494 a         1305 c         1438 g         1603 t
ORIGIN      
        1 aatcgaaatc agtcgatacc cgcagtatat atcgtgaact gggcgcaacg ctgagttaca
       61 acatgcgtct gggaaacggt atggaagttg agccgtggct gaaggcggct gtgcgcaaag
      121 aatttgtcga tgataatcgg gtgaaagtga atagtgacgg taattttgtc aatgatttgt
      181 cgggcagacg tggaatatac caggcaggta ttaaagcctc attcagcagt acgttaagcg
      241 ggcatctcgg ggtggggtat agcaacggtg ccggtgtgga atccccgtgg aacgcggtgg
      301 ctggtgtgaa ctggtcgttc tgaccaccaa cgattaaact gcgcttcggc gcagttttcg
      361 tttacaggat gttgaaagag aaaattctgg ggcaaaaaaa gcccgccagt tacggcggga
      421 aacctcatcc tatgggagaa caatgaataa tgaaattgcg gggttatcat ctcccagtat
      481 atccacacta acaataaggt tatttactca accaggcata aacattttgt tctgtgcgtg
      541 ggaacagcct taaggtgtaa agagggaggt ggaaatagca atgaggagta tcagcaagaa
      601 tactcgccgc tttaccacaa cgtcgatgag agggatgaaa aactcaaggc agagatgact
      661 ctgccttgaa gataaatgcg cttttacagc gggcctattt cagctcttct gcttccggca
      721 aggtcacgtt cagctcaaga atagaaatat cgccatcttt ttgctcaagc tgtacggtta
      781 ccatctcagg atcaatttgt acgtatttac aaatgacctc aagaatatct ttacgcaact
      841 gcggcagata atgcggttct gcatcgctgc gacggcgttc agcaacaata atctgcagcc
      901 gttcttttgc aatgttggct gtgtttttct tccgcgagag aaagaaatcg agtaatgcca
      961 taacttatcc tccgaacaag cgtttgagga agcctttctt ctcttcttca atgaagcgga
     1021 aaggacgttc ttctcccaac agacgttcta cggtatctgc gtaggcttta cccgcatcgg
     1081 cgttaatgtc gagaatgacc ggttcaccct ggttagaggc gcgcaatact gattgatcct
     1141 ctgggatcac gccgacgagt ttgatgcgca ggatctccag cacatcttcc atgctcagca
     1201 tgtcacctct gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc tctttaatag
     1261 gctcttcgcc attttctgcg cggcgtgatt ttgacgccag aatgcctaaa atacggtcag
     1321 agtcgcgtac tgaggagact tccgggttgg tggtaataat ggcttcgtct gcaaaataga
     1381 gtgccattaa cgcaccggtt tcaatccctg ccggggagtc acaaacgata aattcaaaat
     1441 ccatcgcttt cagatcatca agaactttag cgaccccttc acgggtgagg gcatctttat
     1501 cgcgtgtttg cgatgccggc agaatataga gattttcagt acgcttatct ttaattaacg
     1561 cctgatttag cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg acccggcgtt
     1621 cgcaacccat aatcaggtcg agattacgca ggccgatatc aaaatctatc acgacagttt
     1681 tctttccctt ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc ttaccaacac
     1741 cccctttgcc cgaagtaaca acaataatgc gtgccataga aattccttgt taaaaaggga
     1801 tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg ccgctttgcc
     1861 ataaaattct gctgggattt gatcactcag ccagtattca cctgcgatgg acaccagttc
     1921 cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc ctgccagcgc
     1981 acgaccgcgc atcatgccat agacatgaat gttaccatcg gcaattaatt cggccccagc
     2041 gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca taaatacgct gaccggaacg
     2101 caccggggta tctattaaac gcgtttttgt gaccggcgtt gtattttgcg ctggagcctg
     2161 cggtgcggga gctggacgtg gcgctttttc ctttccttcc gtcaggatag gcagccccat
     2221 tttttcaatt tcggctttaa gttgcgcatc tttgcagcca cttacgccaa taacccgcaa
     2281 accggttgcc gaaaccgcct tatgcatcgc tgaccagttt accgggtctt ccagtgcact
     2341 gacgttgagt acaacggggg catgttttaa aaatgcgggg gcctgagcga ttttgtcttc
     2401 cagcgcctga tggataacct taggttctgc ctcatgcaga tgaaccacag ataaagtgaa
     2461 gctactgcct ttaagctcga ttggcgtgtt tgacatcctg gccttactca attagctatt
     2521 aatcatcgcc agcgcgcgat gatgttccga agactataag gcatgttata gtctggatta
     2581 tattgaggca agtcaccctc ccatttattc agagtaaaag tctattctgt gataaatggc
     2641 gctgattcat agcttaaaaa atacccttgt caatcaaccc attgccgtcg tacttttgat
     2701 tgttcttatt tacgcttcct ttttccgcac cctaatcgtg acagcatctt tagacctgtt
     2761 taaatatcca gtattgaggc aagtgtattt tttcaccatg ccaggctttc ttaactttcc
     2821 attttttgca gcatcattaa agaacagata cccgttatct taatatccgc cacagaattg
     2881 ctctatcata gaaaccgcag aagacgtctg atacttctgt caggtcatcg taactcgtcc
     2941 aggtcagcgg ttttgattac ggattgatga tgaaatgagc ataagtgttg tacggttgga
     3001 ttatcaggat gtgctatgaa tatgatgcgt attttttata tcggattgtc aggtgtgggg
     3061 atgatgttct catcaatggc gtctggacat gacgctggcg gacttcaatc tccggcgtgc
     3121 ggcgttgttt gcgacccgta tatttgtgtg aactcagatg gcatttcccc agagttaaca
     3181 aggaaatatc tcggcgaaaa agccgctgaa aacttacaat cattacaagg ctacgatccc
     3241 agcgaattta cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt atgtcgtgat
     3301 gatcgttatt ttggtgtgga tggtaagcgg agtggaaaaa tcaatcaaac caccacaaag
     3361 atgttattta tgtgtcgtga gtgatgcctt gtttacatta tgttctattc ggcattgtct
     3421 gctattcgtc attaacctct tgagcgacag agaggagtgg ggcgttaacg tttatgacgg
     3481 ggagagtccc cgtcggttga catttactta atttgaatat cgacgttata ttttttagtt
     3541 ttgtttttac gggcgtcgtt tcgggtttgc agcactcgca gctcatattt gccagaagca
     3601 gggagtgagt attgtccgtg actatcgagt tctggggaat atctggataa atcaacagaa
     3661 tcatcgattc ctggcccgaa gaggtaagta tcagcacctt cattagagat actcacatgc
     3721 actttctgac cttttttggc atagaaggta tatgtatcgt aatcgtatcc ctttatttcg
     3781 ccggagtact gagcgctgct atggcctttt ctgaactcaa cattgacatt cttacccgca
     3841 gcaaagctcg tggaggttag cagtgcaagc agcaatacag ccttactgat gctcttgatt
     3901 ttcattggta gtctcctgtt cagaatgagt acgatgaact gttataatat aacaatccct
     3961 tacgggagaa ttccagtggc tttcctgcgt tcggtaaaat cccgtcgcgg catttttgcc
     4021 ggggggatta cttataaagg ggcatggact caacaaaccg tagaaatcta cagctggctg
     4081 gcttgtggcg cgggtttcat tgatgttagc ttatgccaaa acctggcatc ttaaagagta
     4141 aaagtatgtt ttgtgtgatt tatcgaagca gcaagcgtga ccagacctat ttatatgtcg
     4201 aaaaaaaaga cgatttttcg cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc
     4261 agttagcgat gattctgccg ctggatgggc ggaagaaact ggtcaatgcc gatattgaaa
     4321 aggttaaaca ggcattaacc gagcaaggtt actatttgca gttaccgcca ccacccgaag
     4381 atttgctgaa gcaacatctt tccgtcatgg ggcagaaaac agacgacact aacaaataac
     4441 cgatatccgg cggtggcatt atctttgtcg gcgcgggttt tcatatccac gataaggtga
     4501 ggggaacgtt atgtatcaac atcacaactg gcaaggtgcg ctgctggatt atccggtgag
     4561 taaagtagtc tgtgttggca gtaactatgc caaacatatt aaagagatgg gcagcgcagt
     4621 gcccgaagag ccagtgctgt ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc
     4681 attggcgatc ccatccgatt tcggttcagt tcatcatgaa gtcgaactgg cggtgttgat
     4741 tggcgcgacg ctgcgtcagg ctacggaaga gcatgtccgc aaagccattg ccggttatgg
     4801 cgtggcgctc gatctgacat tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc
     4861 gtgggaaaag gctaaagcgt ttgataactc ttgtccgctt tccgggttta ttcccgcggc
     4921 ggaatttacc ggcgacccgc aaaatacaac gctgggcctg agcgtaaacg gcgaacaacg
     4981 ccagcaaggt acgactgcgg acatgatcca taaaatcgtt ccgctgatcg cttatatgag
     5041 caagtttttt accctcaagg ccggtgacgt tgtgctgaca ggcacgcctg atggcgtcgg
     5101 cccgttgcaa agcggtgatg agctgacagt cactttcgat gggcattctt tgacaactcg
     5161 cgttttgtaa tactttttgc cgcctgaaag cggcggcaaa acttgcatcg ctgtgccaga
     5221 ctggttataa ggtgcgtttt aacgtaatgg cggaacacct gatgagcgat gtacctttct
     5281 ggcaaagtaa aaccctggac gaaatgagcg atgcggaatg ggagtcgttg tgtgatggtt
     5341 gcggtcagtg ttgcctgcat aaactgatgg atgaagacac cgacgaaatc tacttcacta
     5401 acgtcgcctg tcgccagctc aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt
     5461 tcgagtttga acccgactgc attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc
     5521 tgccaatgac ctgcgcttat cgtttgctgg cggaaggtaa agatttacct gcgtggcatc
     5581 cgctacttac tggttcgaaa gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg
     5641 cagtgaaaga atcagaagtg attgactggc aggatcatat cttaaataaa cctgactggg
     5701 cgcagtgata aatttaatag atgttgatgg gggctttctt aatatacgtc tggatgctcg
     5761 ccatttcgta gccctgtttt tgaaagcgct ctgtcataat gagagttacc cgatacctta
     5821 ttatcaacag gtaactctca
//
LOCUS       DAZGBC010000242         5829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGBC010000242 DAZGBC010000000
VERSION     DAZGBC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.244"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..612
                     /locus_tag="ACHK1J_004720"
     CDS             <1..612
                     /locus_tag="ACHK1J_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7913885.1"
                     /translation="PGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGNEQLYMHAQKN
                     MDTEVLNNRTTDVKVNHTETVGGNQAISVTGVQSTSVKSAQFTHVESLRQVAVQQGQL
                     IVTGGDHEILVQETLHLAAGKAIHLQCGESELVMLANGTVALSCTNFNLYAAQRGQIT
                     AAQILDLNMDGAKSGIEQPGPLTPSAISERVTAEFPPQQPENK"
     gene            612..1040
                     /locus_tag="ACHK1J_004721"
     CDS             612..1040
                     /locus_tag="ACHK1J_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO7913886.1"
                     /translation="MPDYHFNEGQITLPASGRDCTTHVLRLPELQAVLTISRDILSGT
                     MTLEEYVADQIAKIKRDARHVQIPEPQFTDMKNGKHVCQFSCQAEISGLVHYQQQHII
                     LSGQSLLAFAWTLGHPFTEQDHQTWRNILASFVPRETVTE"
     gene            1045..5685
                     /locus_tag="ACHK1J_004722"
     CDS             1045..5685
                     /locus_tag="ACHK1J_004722"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7913887.1"
                     /translation="MGTDYLAAKMEDPLEHTSVLGDFMGGLVEGVCYGVLFCASANPV
                     GLAVGLTAGFIFSDVISEIGDTVSSWFPPDVAGTITSGSKNVNVKSKPAARAAGAVPD
                     EILLALLEAEAANPPDSKGMQLVKGIVAKSIMIAKLPLLPAELGRMAGEKLAAYLKGE
                     STPSQEDEQGFWSRVWESISNPVVDGPDPALHVETRLLDTIDCHKHRPQPIQYLAEGS
                     ETVRINSQPACRNGDRSTCEAVIAHTQASAKVRIGGRSLVVKEIHTGKNMLAYYAGLI
                     AGGFLVSSVQALFCRTTAREFAGKLPCLMLGSVLGNTLGIVAGGTLSTSLSSLSSGLQ
                     ASSQTARPVHIASGVKILGGEDELDFACDGLIPLAWQRIYSSLNDNEGILGRGWCLPF
                     EVFLHQEPAVPGAQDSAQIFFHDMSGRELGLGDVPVGGAMFYVDEGFRLYHPLAGMFV
                     LESAQGQYQIFEADPLHDGRFRLIQECDRHLNGIRYYYNDRGLPVRIEDDLQAICLHL
                     DYHPQHSRLTAVYQQYRQEDTLPGVLRIRYEYNEQVQLTAVVDADGVVMRRFAWDEEH
                     GRLAMHQTADGLQAHYRWQPTSGLPGHFHVARHWLQEGERRSGEILFDYDPAARRATL
                     HYGDGRPDSLHEWDEQYNITRWQPAPDICWLYEWGDNRELLRVVDPAQCEYGFKYDER
                     GNLIQVVDPVGQEYRFDWDRDFAFPLKEEYPEGLVLRHVYNVSGDRIKTIDQAGLITR
                     YNHDASGLVESVQDARGNLYRYAWNNRGQLTRQTDCSGYTTHLYYDEYGRLAAVTDAQ
                     GNCTRQTFSAAGRLQKQIRPDGGETTCHYNARGQLAGVTDPARQSSRFDYNLRGQVIR
                     HTDPQQRQVRFEYDAGGQLIALYNQKNEAYRFSRDSLSRLEESCDLTGCREVYRYDSR
                     SHVSEITRYPAPAVSGQETFLPQVTRLAYDAAGRVQYRENGDGAVCYRYGKNTLSLER
                     FAPDDWQKKQRGYENIAAQDTLHLVTDAYGSLLEEHNPAGIFTHRYDELGNRTETQMP
                     DGRRLKRLYYGSGHLQQLNLEDNDGQQTVLAEFERNSLHQEISRTQGSLTLRTEYDAA
                     GRITARLAQMAQQRHGTPLLERRYSWDQRGFLMSRYHRTQDAQALLSPEERSGSTWYQ
                     YDCAGQILSAQQHFTVRQYCDLAGNLMEERSPAIKNNQLMQYRGWHYQYDAFGRMSRR
                     TDEQAQRQDYRYNSDNRMVEVRFADRTHSRWQRVEYDYDLLGRRTGKRLYRWHDEGEA
                     EPAPERVRFIWDGMRLRGEDSPGKATEVLYVYNGDSYEPLARVDRFTRQVIEPQDTRP
                     PKTTVYYYHTALNGLPEAMTSESGQLVYEARYELWGKETEAASAHTVLTVGQNLRFAG
                     QYFDAETGLHYNTFRFYAPECGRFTQPDPIGLRGGLNLYSYVPNPLSWIDPLGLKCWS
                     TEQKNYWKDVGAKELTNPSGLYSPRNILEMIAGRAPKFTARVKVIKTGKIETRDIPYE
                     LHHKNIPQRQGGPGVHNKSNLDEVDPWQHQDTDKYRHSGIELIEVITGVNSW"
BASE COUNT         1391 a         1461 c         1750 g         1227 t
ORIGIN      
        1 cccggcagcc tgccggggac aaagacgcag atgaccatcc gttcgaagac gtatatgggc
       61 agcgggttta atgagctgaa gttcgatgat gcaacgggta atgagcagtt gtacatgcat
      121 gcccagaaga acatggatac cgaggtgctg aataaccgga ccacggatgt gaaggtaaac
      181 cataccgaaa cggtgggagg caaccaggct atctcagtga ctggggtgca gagtaccagc
      241 gttaaatctg ctcagttcac gcatgtggaa agtttacgtc aggttgcggt gcagcagggg
      301 cagttgatag tgaccggcgg ggatcacgaa attctcgtac aggagacgct gcatcttgcc
      361 gcaggaaaag ccattcatct gcaatgcggt gaatctgagc tggtcatgct ggccaacggt
      421 acggttgccc tgtcctgtac caatttcaat ctttatgccg cccagagagg acaaattacc
      481 gcagcccaga tactggatct gaatatggac ggcgcaaagt caggtatcga acagcccggt
      541 ccactgacgc cgtcggctat ttctgagcgt gtcaccgccg agtttccccc gcagcaaccg
      601 gagaataaat aatgccggat tatcacttta atgaaggaca gattacgtta ccggcttcag
      661 ggcgggactg taccacccat gttttgcgac tgccggaatt acaggctgtg ctgaccatct
      721 cgcgggacat tctgtcagga accatgacgc tggaagagta cgtcgccgat cagattgcaa
      781 aaattaagcg cgatgcacgc catgtgcaaa tcccggagcc gcagtttact gatatgaaga
      841 atggaaaaca cgtctgccag ttttcctgcc aggcagagat ttccgggctg gttcattacc
      901 agcaacaaca cattatcctg tccggacaat cgctgctggc cttcgcatgg acgctcggtc
      961 atccgtttac tgagcaggac caccagacct ggcggaatat cctggccagt tttgtcccgc
     1021 gtgagacggt aacggagtaa tgtcatggga acagattatc tggcggccaa aatggaagat
     1081 cctcttgagc acacttctgt cctgggggat ttcatggggg gcctggtcga aggggtatgc
     1141 tatggggtac ttttttgtgc ctctgccaac cctgttggac tggcggtagg gctgactgcc
     1201 ggctttattt tcagcgatgt tatttctgaa atcggcgata cggtcagtag ctggtttccc
     1261 ccggatgttg caggtacgat aacgtcaggc tcaaaaaacg ttaatgtgaa atccaagcca
     1321 gccgcccgtg cggcaggtgc agtgccggat gaaatattac tggcactcct ggaggcggaa
     1381 gcggccaatc caccggacag taaggggatg caactcgtta aagggattgt ggcaaaaagt
     1441 attatgattg ccaaactacc tcttttgccg gcagaactgg gcaggatggc aggcgagaaa
     1501 cttgccgctt acctcaaagg ggaaagtact ccgtctcagg aagatgagca gggattctgg
     1561 agtcgggtct gggaatccat tagcaacccg gtggtggacg gccccgatcc ggctttgcat
     1621 gtcgagacac ggctgctgga cacgattgac tgtcataaac atcgcccgca accgatacag
     1681 tatctggcgg aaggttcaga aaccgtacgt attaacagcc agcccgcctg ccgtaacggt
     1741 gatcgcagta cctgcgaggc ggtgattgcc catacccagg ccagtgcgaa agtccgtatt
     1801 ggcgggcgca gcctggtggt gaaggaaata cacaccggca aaaatatgct cgcttactat
     1861 gcagggctta ttgccggagg atttctggtc agctcagtgc aggctctgtt ttgcaggaca
     1921 acggccaggg agtttgcggg gaagttgccc tgcctgatgc tgggttcggt cctgggcaat
     1981 acgctgggga tagtggccgg aggaacactt tccacttcat tatcgtccct gtcttcaggg
     2041 ctgcaagcgt catcacagac cgccaggccg gtacacatcg cctccggtgt aaaaattctg
     2101 ggcggagagg atgagctgga ttttgcctgt gacgggttaa tcccgctggc atggcagcgg
     2161 atttacagca gccttaatga caatgaaggc attcttggcc ggggctggtg cctgccgttt
     2221 gaagtgttcc tgcatcagga gcctgctgta ccgggagcgc aggacagcgc gcagatattt
     2281 tttcatgaca tgtccggcag ggagctgggg ctgggagatg ttccggtcgg cggagccatg
     2341 ttctatgtgg acgaaggttt ccggctttat catccactgg ctggtatgtt cgtgctggaa
     2401 agcgcacagg ggcagtacca gatttttgag gcagatcccc tgcacgatgg tcggttccgc
     2461 ctgattcagg aatgcgatcg ccatctgaac ggtatccgtt attactacaa tgaccgggga
     2521 ctgccggtgc ggatcgagga tgatcttcag gccatctgcc tgcatctgga ctatcacccg
     2581 cagcattccc gtctgacggc cgtataccag cagtaccggc aggaggatac cctgccgggc
     2641 gtactgcgta ttcgttatga atataatgaa caggtgcagc tcaccgccgt cgtggatgct
     2701 gacggggtcg tgatgcgccg ttttgcctgg gatgaagagc acgggcgcct ggccatgcac
     2761 cagacggcgg atggcctgca ggcgcattac cgctggcagc ccacttccgg tcttcccggc
     2821 cattttcatg ttgcccggca ctggctacag gaaggcgagc ggcgcagcgg ggaaatcctg
     2881 tttgattacg atccggcggc gcgccgggcc acgctgcatt atggtgatgg aaggccggac
     2941 agcctgcatg aatgggatga gcagtacaac attacccgct ggcagcctgc cccggatatc
     3001 tgctggctgt atgaatgggg ggataaccgg gaactcctgc gggtggttga tcccgcacag
     3061 tgtgagtacg gcttcaagta cgatgagcgc ggcaacctga tccaggtggt tgatcctgtc
     3121 gggcaggaat accgatttga ctgggaccgg gattttgcct ttccgctgaa agaggaatat
     3181 cccgaagggc tggtcttacg ccatgtttac aatgtctctg gggatcgcat caaaaccatt
     3241 gaccaggcag gcctcatcac ccgctataac catgatgctt ccgggctggt tgagagcgta
     3301 caggatgcac ggggcaacct gtaccgctac gcctggaata atcgtgggca gttaacccgg
     3361 cagacggact gttccgggta taccacccac ctgtattatg atgagtatgg acggctggct
     3421 gccgtgacgg atgcacaggg taactgcacc cgccagacgt tttcagctgc gggccgcctg
     3481 caaaagcaaa tccgccctga tggcggggaa accacctgcc attacaatgc ccgtggacag
     3541 cttgccggtg tgaccgatcc ggcacgtcag agcagccggt ttgactataa cctgcgcggg
     3601 caggttatcc gccacactga tcctcagcag cgccaggtgc gcttcgaata tgatgccggc
     3661 gggcagctta ttgcgttgta taaccagaaa aatgaagcgt accgtttcag ccgggacagc
     3721 ctgtcacggc tggaagagtc ctgcgatctg acggggtgcc gcgaggtcta ccggtatgac
     3781 agccgcagtc atgtcagcga aataacccgt tatcccgccc ccgccgtatc cggtcaggag
     3841 acgtttttac cgcaggtcac ccggctggcg tatgatgccg ccggacgggt gcagtaccgg
     3901 gagaacggag atggcgctgt ctgttaccgg tatggaaaaa acacgctgtc acttgagcgg
     3961 tttgcgccgg atgactggca gaagaaacag cgcgggtatg aaaatatcgc tgcgcaggac
     4021 acgctgcatc tggtcacgga tgcgtatggc agcctgctgg aagaacataa cccggcgggc
     4081 atctttaccc accgctatga cgaactgggc aaccgtacag aaacgcagat gccggatggt
     4141 cgccgcctga agcggctgta ctacggcagc ggtcacctgc aacagctcaa cctggaagac
     4201 aacgacgggc agcagacggt actggctgag tttgaacgta acagcctgca ccaggaaatc
     4261 agccggaccc agggcagcct gacactgcgc acggaatatg atgccgccgg gcggattact
     4321 gcccgtctgg cgcagatggc gcagcagcga catggaacac cgttgctgga gcggcgttac
     4381 agctgggatc agcggggctt cctgatgagc cgttaccacc ggacacagga tgcgcaggca
     4441 ctgctttccc cggaggaacg cagtggcagc acctggtacc agtatgactg cgccgggcaa
     4501 atactgagcg cacagcagca cttcacggtg cggcagtact gcgatctggc cggaaacctg
     4561 atggaggagc gcagtccggc gatcaagaat aaccagttaa tgcagtaccg gggctggcac
     4621 taccagtatg atgccttcgg gcggatgagc aggcgcacgg atgaacaggc gcaacggcag
     4681 gattaccgct ataacagcga taaccggatg gtggaagtgc ggtttgcgga caggacgcac
     4741 tcccggtggc agcgggtgga atacgactac gatctgctcg ggcgacggac ggggaagcgg
     4801 ctgtaccggt ggcatgatga gggcgaagcg gaaccggctc cggaaagggt gcgttttatc
     4861 tgggacggga tgcggctgcg gggtgaagac agcccgggta aggccacaga agtcctgtat
     4921 gtctataacg gcgacagtta tgagccgctg gccagggtgg atcgttttac ccggcaggtg
     4981 atagaaccgc aggacacgcg cccgccgaaa accacggtat attactacca cacggcgctg
     5041 aacggcctgc cggaagcgat gaccagtgaa tcagggcaac tggtctatga agcgcgttat
     5101 gaactgtggg ggaaagaaac ggaggctgca agcgcacata cggtcctgac agtggggcaa
     5161 aacctgcggt tcgcggggca gtattttgac gcggaaacgg ggcttcacta taatacgttc
     5221 agattttatg cgccggaatg tgggcggttt acccagcctg acccgatagg gctaagaggc
     5281 gggctgaacc tctatagtta tgtaccgaat ccgctgtcat ggatcgatcc acttggtctt
     5341 aagtgttggt caacagaaca aaaaaattat tggaaagatg ttggtgcaaa agaattaacc
     5401 aatccatcag gattatattc accaagaaat attttagaaa tgatcgctgg gcgtgcacct
     5461 aaattcacag ctagagttaa agtaattaaa acgggtaaaa tagagacaag agatatacca
     5521 tatgagttac accataaaaa cattcctcaa aggcaaggtg ggcctggtgt tcataataag
     5581 agtaatttgg atgaagtcga tccttggcaa catcaggata cggataaata cagacactca
     5641 ggaattgaac ttattgaggt tataacagga gttaactcat ggtaattgat aataactata
     5701 ttttattcgg tgcggcattg tccgagatag cgacgaattc atataatgct ggtatcggtc
     5761 acaaagagtc agagttgttt ataaaacgta tttatgatga atatttacta acatctaatc
     5821 ctgcaccag
//
LOCUS       DAZGBC010000243         5778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGBC010000243 DAZGBC010000000
VERSION     DAZGBC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.245"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..663
                     /gene="ireA"
                     /locus_tag="ACHK1J_004723"
     CDS             <1..663
                     /gene="ireA"
                     /locus_tag="ACHK1J_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO7913888.1"
                     /translation="IMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLT
                     SYDIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDD
                     ESLNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPR
                     VRNGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRI
                     SF"
     gene            1329..5645
                     /locus_tag="ACHK1J_004724"
     CDS             1329..5645
                     /locus_tag="ACHK1J_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7913889.1"
                     /translation="MNLRKKHICIAISLVLNPASISQASAQADTNKLTVEVDETISSA
                     GDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEGLYT
                     GESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENNYLA
                     SEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMATAVGFNAL
                     ANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGADSRD
                     KQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAVSEKSTD
                     AINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDGDIA
                     VTVNDKGMTIDGTKLTENIKTDAIKIKDNNGGLLKKDLSDEIEITGKGPIKTQVAGDK
                     LEISAETASLPVNGGNGKITVNKGDDDKLVTAKKIADAINQSGWKIKAGSENGGKVEG
                     GNKEELINPGDTVTLKAGKNLSVKQSGGDFTFSVIDTPSFSSVQLNGKGNNGSVTLSS
                     EGNKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLKTAIDGV
                     SSSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITNGDKGLKISLNNDLSVG
                     SNNTPGSIKVNGADGKEAVSLNGQDGTIKLTSAPAANGKSASATIAVNAGKGGLTDAA
                     GTTKTRLTYAPDGTLPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKGGAEKNAEVT
                     DGNIRVDNDNGGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITITTPAAGGGQGNTV
                     SLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSAALMNTG
                     FGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVGGT
                     RMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATAATTW
                     ALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGIPVEYTDSQGN
                     PLVNIGGRFYTQTEDAATGKLTMTPAEPARVRISSEQPLVLTNVAAGNVSPVSTDAVN
                     GSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADAV
                     NMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAAGS
                     VAVGSHARAGRANTGTYALNGQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSANS
                     TDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSGIP
                     QAYLPGKSLMGLGIGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAGVGW
                     QW"
BASE COUNT         1671 a         1104 c         1593 g         1410 t
ORIGIN      
        1 attatgtatg gaaacaggga cctgaaaccg gagaccagtg taaccgaaga gatcggtatt
       61 atttataata atgacagtgg tttttcagca agcgcgactc tgtttaatac agattttaaa
      121 aataagctga ccagttacga tataggcaca aaagatccag tcactgggct aaacactttt
      181 atttatgata atgtgggtga ggcaaatatc agaggtgtgg agcttgcaac tcagattcct
      241 gtgtatgata aatggcgtgt atctgcaaac tatacattca ctgactcccg tcgagaaagc
      301 gatgacgaaa gtctcaatgg caagtcgctg aaaggggaac ctctggaaag aactcccaga
      361 catgcagcca atgcaaaact ggaatgggat tacactcagg atattacatt ttattcatct
      421 ctgaattata caggaaaaca aatctgggca gcacaaagaa atggtgccaa ggttccccgt
      481 gttcgtaatg gattcacttc tatggatatt ggtatgaatt accagttcct gccaggcacg
      541 ctgattaatt ttgccgtact taacgtcaca gacagaaaga gcgaagatat cgataccatt
      601 gatggtaact ggcaggtcga tgaaggacgt cgttactggg ctaatgtaag aatatccttc
      661 tgagagaata tagataacct gaggccgtgc gcctcaggta ccagtatagt aatcagtaac
      721 gtcagcttcc aggttgtaat gcttttaatt ttgataaaaa taaacttaat aaattacatt
      781 tgttaaatat tgataatttt aatttggttt tattactcca gttatactca cactctatct
      841 ttacacaatt catcaccatt acacctccat agcctgttga tttaaaagat ttaatttcct
      901 gtgaaacaat caggcctgta tgaacctgat tataccacat aatcttttaa cttatttaaa
      961 tttaactctc aggcttttct gtcagtaaaa tcagaaagaa ttgcaggatt tcgttttttt
     1021 gctgcgaagt gaaatgtatc tcatttttac tgccttcgcc agtgcgtcag gcaatggcag
     1081 gtagtgtacc tgcattctgc tgtaacgcgt aatgtcgggt attttactta atatgtttta
     1141 tgtttgcatt attataaatc actttattgc cttttgtctg ctttttaatg gttcccgttt
     1201 ttttacttct ggctttatat aaatggatgc tgaagaaatg aaggcagtcc gccttacata
     1261 atattcatgc atgattttta tgtcattccg actgagtgga agaaaataaa ggagaaaaga
     1321 ggatatatat gaacctcaga aaaaaacata tctgtatagc catatcactt gttcttaatc
     1381 cggcatccat atcgcaggcc agtgctcagg cagatacaaa taagttaact gttgaagttg
     1441 atgaaaccat cagttcggca ggcgatgact ggaagagtat tgaaataggt acaacgaaat
     1501 ttacagcaaa agatgaatca aagggaatta atttcgacca taaatcaccg gtggctgaag
     1561 gacgtaactc tatcgcgatt ggtaccagtg cagaggctgg aggtgacatt agcacgaagg
     1621 aaggtttgta cacaggcgag tctattgcga taggagcgaa aacgaaagca actaaagaac
     1681 agtcaattgc aataggtggc gataccaaag ctaccggatg gggggctatt gttattggtg
     1741 gggatgattt aactcctttg catggtaaga cttatggtcg ggaaaaaata gagaataact
     1801 atctggcatc ggaagcgaag ggggatggca gtattgcgct gggcagcaga acttttgcta
     1861 aaggcgaatt gtcagtatcg cttggttcgg tgagttctgc tgaggggatg gcatcgaatg
     1921 caattggagc cacggcggaa gctaaagggg atatggcgac tgcggttggt tttaatgcgc
     1981 tggctaatgc cggtaatgct ctggccgttg gtagtcagtc ctctgcaaat aaaaacgatg
     2041 ctcttgctgt tggttcagag gcaaatgccg ctcagaatga aacggttgcc ctggggcatg
     2101 gagcaaccgt tactgtcgca aaggggaacg tgtccatcgg cgctgactcc agggataagc
     2161 aggcacaaaa agtaacagag gctaaggtca gaactaaaag aggcgcggaa gtcacatata
     2221 gtggctttgc cggaaataac gcagcatcag tagtatctgt tggtgatgcg ggtaaagagc
     2281 gtcagattgt taacgtgggg gcgggtgctg tatctgagaa aagtacagat gccattaacg
     2341 gtagtcagtt atatgcgata gcgaagacgc tgaaagatga ccttgatgcc attaatccga
     2401 cgtggacgct aaaggatgat aagggaggtt ctgcgactat taatggtggg aaggataata
     2461 agaatggtaa cactttaact gttaaaggtg atggagatat tgcagttact gttaatgata
     2521 aaggaatgac gattgacgga actaaactta ctgaaaacat taaaacagat gctataaaaa
     2581 taaaagataa caatggtggt cttctgaaaa aagacctttc ggatgaaatc gaaatcactg
     2641 gcaaaggccc aataaaaacc caggtggctg gagataaact ggagatttca gcagagacag
     2701 cttcgttgcc tgtaaatggt ggtaacggga agattacagt aaacaaaggt gatgacgata
     2761 agcttgttac tgcgaaaaag attgctgatg ccataaacca gtctggctgg aaaattaaag
     2821 ctggcagtga aaatggtggg aaagttgagg ggggtaataa agaggagcta attaatcccg
     2881 gtgatacagt tacactgaaa gccggtaaaa atctcagcgt gaaacaaagt ggtggtgatt
     2941 ttacattctc tgttattgat actccgtcat tcagcagtgt tcagctgaat ggaaaaggaa
     3001 ataacggaag tgtaacgctc tcatccgaag gtaataagct gacattgtca ggcggtaatg
     3061 gtgccggaca aaatggtgtt gttatcagtg gtgtggcaag tggactggaa ggtaaggaac
     3121 tttcgacgtt aacgaatact gataaagaga tgaaaaatgc cgccagtgtt gcagacctga
     3181 aaacggcaat tgatggtgtg tcctccagta tgcagggtgc aggtttcgca ctgaaagact
     3241 ctgatgataa ggaagtgaaa cagtctcttg ggcaggcgat taaagtatcc ggggacagca
     3301 atatcaccac cacagccatc accaatgggg ataaggggtt aaaaatatcc ctgaataatg
     3361 acctgagcgt tgggagcaat aatactcccg gcagcatcaa agttaacggg gctgatggta
     3421 aggaggctgt gagtctgaat ggtcaggatg gcacgattaa actgacatcc gcgcctgctg
     3481 cgaacggaaa gtctgcgtca gcaaccattg ctgttaatgc aggcaaaggt ggattaacag
     3541 atgctgccgg taccacgaag acacgtctga cttatgctcc agatggaaca ttaccggcag
     3601 aagagctggc gaccatgaat gacggtcttt cttttgaggg tgatgacgga aaggttattc
     3661 ataaaaaact gaatgaaaaa ctgaccatca aaggtggtgc agagaagaat gctgaagtga
     3721 ctgacggcaa tatccgggtt gataatgata atggcgggct tctggtgagg atgaccagaa
     3781 acctccgtga tttggccagc gcggtttttg gtacaggtgg gcagataaca acagttaatg
     3841 ataaaggcat cacaatcaca acacctgctg cgggcggtgg tcaggggaat acggtttccc
     3901 ttaccggctc cggcctgaat aacggtggaa atcagataac cagtgtggcc agtggtctga
     3961 acggcactgc gcttgctgac gccggtggag atgttctgaa taatgcggcc aatatcggtg
     4021 acctgaaaaa tgctctccgt gacagcagtg cggcactgat gaataccggc tttggtctgc
     4081 aggcggatga cggaagcaag gtgaacacgt ctctcgggaa gcatatcacg gttggtggtg
     4141 acgggcagaa tatcagcacg actgttcaga acgggagcct gacggtcaga ctcgctgata
     4201 acattcgcct ggcagatgac ggcagtgttt ctgtcggcgg aacccggatg gataaaaacg
     4261 ggctgaccat caaggatggc ccgtcagtga ctgtgaacgg tattgatgct gctggccggg
     4321 ttatttccgg cgtgaaagac ggggtgaatg accaggacgc cgttaacgtt tcacaactga
     4381 accgtgaaat cagtcaggca acggcagcaa cgacctgggc actgaagact gaatccggtg
     4441 atgaggctgt tgctgtttct tcccagacag ttgaagtccg ccacggacag aatacccggg
     4501 tttcagccat cagtaaggat gataagggaa attacagcta tgagattgat gtgacgggga
     4561 ttccggtgga gtacaccgac agccagggga acccgcttgt caatattggt ggtcgttttt
     4621 acacacagac cgaagatgct gcgaccggga aactgacaat gacgccggct gagccggccc
     4681 gtgtcagaat aagcagtgag cagcctcttg ttctgacaaa tgtggcggcc ggaaatgtgt
     4741 caccggtatc cacggatgcg gttaacggga gccagctggc atctgcagcc agaattgctg
     4801 gcggtgataa tgttgtctgg aaagacggaa aagcagcgct ggctcctgac accttcagtg
     4861 agctgacaac tgcaggtggc ggtaagacaa cggtgaatgg tggtgtacat aaaacaccgg
     4921 ataatgtggc tgatgcagtg aatatgctga accgtgaagg cacgaagtat tttaaagctg
     4981 cctcttccgg tcaggcagca aaagcggaag gacgtgacag tattgctgtc ggacaggggg
     5041 ccgtttcccg gggacaaaac agtattgcca tgggtaatgg tgctgaagtc acgcgtcagg
     5101 ctgctgccgg ctctgttgca gttggcagcc atgcccgtgc tggccgcgcg aataccggga
     5161 cttatgccct gaacggtcag gctgttgcgg gacgtaccgg aagtgatact gcggtggttt
     5221 cttttggtca gccgggacag gagcgtcagc tgcagtcagt ggcaccgggt gtgttgtctg
     5281 cgaattcaac ggatgccgtc aatggcagtc agttgcatgc aactaaccgt caggttgccg
     5341 gaaatacaca ggcgataaac actctcggga ataaattcag tcagctgagc taccgggtgg
     5401 aagagcttaa ccgggatatc cgtggtgttg gcgcatcggc tgcggccatg agcgggatac
     5461 ctcaggccta tctgccgggt aaaagtctga tggggctggg tatcggtgga tatggtggtg
     5521 aaagcgccat tgcaataggt gtgtcccgaa tctccgataa cggtaagatg ataatgaaac
     5581 tgaatgcagg tcagaacacc cggggtaatt tctccgtcgg tgccggtgtg ggctggcagt
     5641 ggtaacaggg aaaagcaagg agaaatgtaa tgttatttag tcgaatgatg atgacgggtg
     5701 tgctgtccct gatgcttgca ggatgtggaa atctgagtca tgtcacggat gaaggaacga
     5761 cggaagaagt ggtatggc
//
LOCUS       DAZGBC010000244         5740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGBC010000244 DAZGBC010000000
VERSION     DAZGBC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.246"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..1249
                     /gene="nhaA"
                     /locus_tag="ACHK1J_004725"
     CDS             83..1249
                     /gene="nhaA"
                     /locus_tag="ACHK1J_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO7913890.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1315..2214
                     /gene="nhaR"
                     /locus_tag="ACHK1J_004726"
     CDS             1315..2214
                     /gene="nhaR"
                     /locus_tag="ACHK1J_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO7913891.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2252..3211)
                     /locus_tag="ACHK1J_004727"
     CDS             complement(2252..3211)
                     /locus_tag="ACHK1J_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO7913892.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLLETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(3224..5674)
                     /locus_tag="ACHK1J_004728"
     CDS             complement(3224..5674)
                     /locus_tag="ACHK1J_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO7913893.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGKWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYTIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     VSNFDFIAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNNWQHISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSLLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
BASE COUNT         1509 a         1201 c         1298 g         1732 t
ORIGIN      
        1 tacactataa tctgatttta acgatgattc gtgcggggta aaatagtaaa aacgatctat
       61 tcacctgaaa gagaaataaa aagtgaaaca tctgcatcga ttctttagca gtgatgcctc
      121 gggaggcatt attcttatca ttgccgctat cctggcgatg atgatggcca acagcggcgc
      181 aaccagtgga tggtatcacg actttctgga gacgccggtt cagctccggg ttggttcact
      241 cgaaatcaac aaaaacatgc tgttatggat aaatgacgcg ctgatggcgg tatttttcct
      301 gttagtcggt ctggaagtta aacgtgaact gatgcaagga tcgctagcca gcttacgcca
      361 ggccgcattt ccagttatcg ccgctattgg tgggatgatt gtgccggcat tactctatct
      421 ggcctttaac tatgccgatc cgattacccg cgaagggtgg gcgatcccgg cggctactga
      481 cattgccttt gcacttggtg tgctggcgct attgggaagt cgtgttccgt tagcgctgaa
      541 gatctttttg atggctctgg ctattatcga cgatcttggg gccatcatta tcatcgcatt
      601 gttctacact aatgacttat cgatggcctc tcttggcgtc gcggctgtag caattgcggt
      661 actcgcggta ttgaatctgt gtggtgtacg ccgcacgggc gtctatattc tggttggcgt
      721 agtgttgtgg actgcagtgt tgaaatcggg ggttcacgca accctggcgg gggtaattgt
      781 cggcttcttt attcctttga aagagaagca tgggcgttct ccagcgaagc gactggagca
      841 tgtgttgcac ccgtgggtgg cgtatctgat tttaccactg tttgcatttg ctaatgctgg
      901 cgtttcactg caaggcgtca cgctggatgg cttgacctcc attctgccat tggggatcat
      961 cgctggcttg ctgattggca aaccgctggg gattagtctg ttctgctggt tggcgctgcg
     1021 tttgaaactg gcgcatctgc ctgagggaac gacttatcag caaattatgg cggtggggat
     1081 cctgtgcggt atcggtttta ctatgtctat ctttattgcc agcctggcct ttggtagcgt
     1141 agatccagaa ctgattaact gggcgaaact cggtatcctg gtcggttcta tctcttcggc
     1201 ggtaattgga tacagctggt tacgcgttcg tttgcgtcca tcagtttgac aggacggttt
     1261 accggggagc cataaacggc tcccttttca ttgttatcag ggagagaaat gagcatgtct
     1321 catatcaatt acaaccactt gtattacttc tggcatgttt ataaagaagg ttctgtggtt
     1381 ggcgcagcgg aggcgcttta tttaacacca caaaccatta ccggacagat tcgggcgctg
     1441 gaagagcgcc tgcaaggcaa attatttaag cgcaagggac gtggtctcga gcccagcgag
     1501 ctgggggagc tggtctatcg ctatgccgat aaaatgttca ccttgagcca ggaaatgctg
     1561 gatatcgtga actatcgcaa agaatccaat ttattgtttg acgttggcgt ggctgatgca
     1621 ctttccaaac gtctggtcag tagcgtgctg aatgctgcag tagtagaagg cgagccgatt
     1681 catcttcgct gcttcgaatc cacccacgaa atgctgctgg agcaattaag ccagcataaa
     1741 ctggatatga tcatttccga ctgtccgata gattctacgc agcaggaagg cctgttctcc
     1801 gtgagaattg gcgaatgtgg cgtgagtttc tggtgtacaa atccaccgcc ggaaaaaccg
     1861 ttcccagctt gtcttgaaga acggcggctt ttgattcctg ggcgacgttc aatgttaggg
     1921 cgcaaattac ttaactggtt taactcccag ggattaaacg tagaaatcct tggcgagttt
     1981 gatgatgccg ctttgatgaa agcttttggt gcgatgcaca atgcaatctt cgttgcccca
     2041 acgctttatg catatgactt ttatgccgat aaaactgtcg tagaaattgg tcgcgtcgag
     2101 aatgtgatgg aagagtacca tgctattttt gctgagcgga tgattcagca cccggcggta
     2161 cagcgaatct gcaatacgga ttattctgcg ctttttagtc cagcggtgcg ttaatcggca
     2221 gctcccccaa agttaaggtg ggggagatag attagttgta cattaccacg attttgactc
     2281 ggctcattat ttgcccgctt gagacattgt ttccatatgt acgcgggcga ataaatagag
     2341 gaatctgatt acttccttca tggggatgct gaaaagagta gtaattgctt ttaccatact
     2401 ggatttcatt cgtaccatca ttggagagtt gaaagcctat ttgacccgca ccgtcctcac
     2461 tgttttctaa ctcatttttt aatatgctgg ttcctgactc ctgtatcgtc actggttcaa
     2521 agtaatattc aacgtcactg ggttcaaaaa ctaattcacc ttgctcgccc tggatgcagt
     2581 caaatcttat atgatgaaac tgataatttt cattattgat acgagacagt acaaatcgtc
     2641 cagtgtcgat ggattgatct tcaaaaccac aggtcattaa atttacctgt aaaatagtat
     2701 ttaaattaaa attaaagcgg acgctattac aattagtatt attccctgta catgaactca
     2761 cttcaatttc gaacaacaaa ggaaaaggag ttcctggaaa aatatctgcg gtgttggaac
     2821 tatctgatac agatgcgata gagtatggaa tggtgaaatt tatcgcagat agcatctctt
     2881 ctaaattata agatgacttc cctggaagct ctattttttt accgtcagat tcaaagggga
     2941 aattaataat ataatgacga cttccgactt taaaccttaa agcgttctgt gttaaaaaat
     3001 gaactttaat ggcgttagtc gtctctagca agtctgcaca ctcaatgata tttccggtgt
     3061 tttcaatttt ttcggcggtg agattacctt tcatactcag tggcttgagt atatatgttc
     3121 cacgttgttc cccgacattg gtcaatccac agggagcact ttgcacaata aaagaatata
     3181 gactcaaggt gattaataaa agccacttca tagtaaactc cttttactgg catatatatg
     3241 ttttgttttc atcgataacg tgttgaaaag taatagtaca gaataaattc tgttcatcat
     3301 tgagagcaac ttttatgcca gtgggaattt catctacgcg aataaaaagg agactaccct
     3361 ggccaacgac tccaatattg ttttcctgga gatccagtac gtcatagcca aaagttaatg
     3421 gcgaaccatc gggacgcagt gcctgtatat accagggctt acgttggtca gttgtaaact
     3481 gcacataaga aactgctcca cggtaaggcg caataatttg acgattgccc tgcaattctg
     3541 tttcactttc actgtttgct gtatcaagta ccagatggtt ttcacgatga ggtatcatca
     3601 ggtcgtaaac aacctggcct ttgctgtttg ttcggttgta tttttgtcca ttaatatgcg
     3661 cgccttctaa cccaggcgca tccagaattg caaatgtatc ggatagctgg ttagtgatat
     3721 taataccacc gggccagaca actaatccac tactaatact tccaccactt tgccaggcgt
     3781 ttttagaatg gctatagctg ccatctattg tggcgattgg agttctccag cttaaattcg
     3841 taccaagcga ggtatcatta ttttgttgtt gctgattaac atatatacca taatttaact
     3901 gatcatgttc accggctatg ccagtaattc cagtattgtt ggaggaatag ccatctttgg
     3961 aaaatgaggt agaattcgat aagttaattt ggtgacgtgt tttggcaata tcatcacccc
     4021 agtagaaagg aatggagata aacagattaa aacgctcctc ttctttatcg ttttcatcat
     4081 aggattggct ggcagagaaa gtataactaa tgtgttgcca gttattggag taactgaatt
     4141 ggtaatcttt agcatttcca cttcgccccc agtaattccg ccaaagtgca cttaatgata
     4201 catttcccag attattggat aaaggttgca ttatattggc agaaaggcta ttttttctgc
     4261 caatatcata aaaatcatca tagtcactct gatgatttat tttatcattt tcgtaaagat
     4321 ggtcgctgaa tgtcctgtaa tcctgcgaag cataacgcca ggcggccacg ctaaagtgag
     4381 tggccgtttg caatagatat ttattatagg cgacctgata gctcgtacct tcgtgtctgg
     4441 tatcattatt taacttacta ctcgatcggg tagcgtcaaa agagatggca cccagcggtg
     4501 tattccaacc atttcccaga gttatagcat tatagttatc ggataaaatc gtaccgccat
     4561 aaagtgtcaa aagattgttg aggccatata tataatttgc ctcgagaaag tcctcctgat
     4621 tcttgacacc atatatctga ctacgtccgg cgataaaatc gaaatttgaa acaccaggtt
     4681 gcagcatatt gggcacggaa gagtagggca ccaaaaagga acgaacacta ccatctgctt
     4741 ccttaatgct gacatcgaga tcagagccgc tgcccgaaag ttgcaaatct gcgatagtaa
     4801 atggtcctgg gggaacctct ttttggtaaa tggtatagcc attttgcgaa accgttatca
     4861 aggcgttact ttgcgcaaca ccttgtacta aaggtgtaaa gctttgcata gaatcaggta
     4921 gcatttgcat atcgcgaaat aagcgtattc cgctaaatcg cagagagtcg aatatgagtg
     4981 aggatgtgta attttctcct atctgaactg tgcttaatat ctgcgaccag ccacgttcca
     5041 ggtaaagagt attactttgc catttgcctt tcatatcatc aggtttgcta taactggcat
     5101 cagagtgtaa ctgccaacta aataaattta atccgctgtt aaaccggcca taactggctg
     5161 tattactgtt attattgctg tcataagagc gatactgact taaattatag gaggtataaa
     5221 aagcatcaat gccgcgatcc caactttccg gaggaacgta acctctttca agttcattga
     5281 tatacgcctg tggtactgtt aaatttagtc ggtgttcact gatatcccac tgatattgtc
     5341 caccatgaac cgctttatcc agtaaaatac aattatcttc tgtattgagg ttaccggtct
     5401 ttacccccag tttggttagt agcaccttag gcagacatgg tttgactggt gactctggga
     5461 tcgtgatatc ttgttttcca cgccactggt tatttacata aaaatcaagt tcgtagtttc
     5521 ctggaagcgg ttgtttatta tcgaaatgat attcggaaac tttttctcct ttcatgccgc
     5581 cgatcataaa gtgcgtatca aaagtttctt cagcataacc cggaaaagaa tacaaaaaaa
     5641 taatagcagc gacccatcgt gtcgtcctta acatgacttt ttcctgttat ctcgcggcga
     5701 ttttactatg gatgttattc ccgtaatcat caactaccgt
//
LOCUS       DAZGBC010000245         5643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGBC010000245 DAZGBC010000000
VERSION     DAZGBC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.247"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..169
                     /locus_tag="ACHK1J_004729"
     CDS             29..169
                     /locus_tag="ACHK1J_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7913894.1"
                     /translation="MRQAVAVMPLAAKVLKQVMVNTGGNKEQVPAKRQNRGSRNSTDD
                     GG"
     gene            193..714
                     /locus_tag="ACHK1J_004730"
     CDS             193..714
                     /locus_tag="ACHK1J_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7913895.1"
                     /translation="MAVRGINKVILVGRLGKDPDVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            772..1005
                     /locus_tag="ACHK1J_004731"
     CDS             772..1005
                     /locus_tag="ACHK1J_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO7913896.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGILK"
     gene            1066..3024
                     /locus_tag="ACHK1J_004732"
     CDS             1066..3024
                     /locus_tag="ACHK1J_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7913897.1"
                     /translation="MSVTKSEPKSTRKASRKLAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVCVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMESVGFCREDAGTYRCLPEPESVLTEAEE
                     ERLNELMARYDALENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDPAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPVGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMGSPRPQTDATERADNLADAA"
     gene            3079..3513
                     /gene="psiB"
                     /locus_tag="ACHK1J_004733"
     CDS             3079..3513
                     /gene="psiB"
                     /locus_tag="ACHK1J_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7913898.1"
                     /translation="MKTELTLDVLQTMSAQEYEDIRAAGSGERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3510..4229
                     /locus_tag="ACHK1J_004734"
     CDS             3510..4229
                     /locus_tag="ACHK1J_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7913899.1"
                     /translation="MSVRSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRWISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKTRAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4226..4540
                     /locus_tag="ACHK1J_004735"
     CDS             4226..4540
                     /locus_tag="ACHK1J_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFO7913900.1"
                     /translation="MKWRYSLRWKLPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            4690..4842
                     /locus_tag="ACHK1J_004736"
                     /pseudo
     CDS             4690..4842
                     /locus_tag="ACHK1J_004736"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            4787..4909
                     /locus_tag="ACHK1J_004737"
     CDS             4787..4909
                     /locus_tag="ACHK1J_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7913901.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(5363..5536)
                     /locus_tag="ACHK1J_004738"
                     /pseudo
     CDS             complement(5363..5536)
                     /locus_tag="ACHK1J_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1223 a         1511 c         1773 g         1136 t
ORIGIN      
        1 aaaaaatttt gcgttctgcc caggacaggt gcgtcaggcc gtggcagtga tgccccttgc
       61 ggcgaaggtt ctcaaacagg tgatggtgaa taccggaggg aacaaagagc aggtccccgc
      121 aaaacgtcaa aaccgcggct cccgaaactc aacagatgat gggggctgaa aacatcaaca
      181 gaaggagaca tcatggcagt tcgtggcatt aacaaggtca ttctcgtggg acgtctggga
      241 aaagatcctg atgtccgtta catccccaac gggggcgcag tggcaaatct gcaggtggcc
      301 acgtcagaaa gctggcgtga caaacagacg ggggagatgc gggagcagac agaatggcac
      361 cgcgtggtgc tgttcggcaa gctcgcggaa gtggcaggtg aatatctgcg taagggcgcg
      421 caggtctaca tcgaggggca actccgtacc cgtagctggg aagataacgg tattacccgt
      481 tacgtcactg aaatccttgt gaaaaccacg ggcaccatgc agatgctggg gagtgcacca
      541 cagcagaacg ctcaggcgca accggagcct cagcagaatg ggcagccaca gagtgctgac
      601 gcgacgaaaa aaggtggcgc gaaaacgaaa ggccgtggac gtaaggccgc gcagccggag
      661 cctcagcagc cgtctgaacc cgcgtatgac ttcgatgacg atattccgtt ctgaacgggc
      721 tgactgtgat aaccgccccg tcctgcgcgg ggcatcaccg gagaaatgag gatgagcgaa
      781 tatttcagaa tacttcaggg actgccggac ggctccttta cccgcgaaca ggcggaagcc
      841 gttgccgcac agtaccggaa cgtctttatc gaggatgatc agggaacgca ttttcgcctg
      901 gttgtccgtc aggatggcac gttgatctgg cgctcctgga attttgagga ctgtgccggg
      961 tactggatga accggtacat cagggatttc gggatcctta agtaagagag gtgccggacg
     1021 gggatcacgt ccggcagaca ttaactacac aataagggga tagtcatgtc agtaacaaag
     1081 tctgaaccaa aatctacccg taaagcaagc cgtaaacttg caaaaacgca ggaaactgtc
     1141 ctgtctgccc tgctggcgca gacggaggaa gtgagcgtgc cgctggcctc actgattaaa
     1201 tcaccgctga atgtgcgcac ggtgccgtat tctgcggagt ccgtcagtga actggctgat
     1261 tccattaagg gagtcggcct gctgcagaat ctggtcgtgc ataccctgcc tggtgaccgt
     1321 tacggtgtcg ccgcaggtgg tcgccgactg gctgcactca acatgctggc agagcgtggc
     1381 atcattccgg ctgactggcc tgtctgtgtg aaggtcattc cgcaggaact ggcgacggcg
     1441 gcatcaatga ctgaaaacgg tcatcgtcgg gatatgcacc ctgctgaaca gattgccgga
     1501 ttccgcgcaa tggcgcagga aggcaaaaca cctgcacaaa tcggtgattt gctgggctat
     1561 tcaccccgcc acgttcagcg aatgctgaaa ctggcagacc ttgcaccagt catactggat
     1621 gcgctggcag aagaccgcat caccaccgaa cactgtcagg cgctggcact ggagaacgac
     1681 accgcgcgtc aggtgcaggt gtttgaagcc gcctgtcagt cgggatgggg cggtaaaccg
     1741 gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag tggcggtggc aggcaacagt
     1801 aaattccgct tcgtgggggc tgatgctttc tcacctgacg aactgcgcac cgatttgttc
     1861 agcgacgacg agggtggcta tgtggactgt gttgcgctcg atgctgccct gctggaaaaa
     1921 ctccaggctg tcgctgaaca ccttcgggaa gctgaaggct gggaatggtg cgccggacgt
     1981 atggagtctg tcggtttctg ccgtgaggat gccggaacat accgttgtct gccggagccg
     2041 gaatcggtgc tgaccgaggc ggaagaagaa cgcctgaacg aactgatggc acgttacgac
     2101 gcactggaaa accagtgtga ggaatctgac ctgctggcag cagaaatgaa gctgattgac
     2161 tgcatggcga aggtcagggc gtggacgccg gagatgcgtg ccggaagtgg cgtggtggtg
     2221 tcctggcgtt atggcaacgt atgtgtccag cgtggtgtgc agttgcgcag cgaggatgac
     2281 ccggctgccg acgctgaccg cacggaacag gtgcaggaga aagcgtcagt ggaggaaatc
     2341 agtctgccac tgctgacgaa aatgtcttca gagcgcacgc tggcagtcca ggcggcgctg
     2401 atgcaacagc cggacaaatc cctggcactg ctggcatgga cgctctgcct gaatgtgttt
     2461 ggcagcgggg cgtacagtaa accagcacaa atcagcctgg aatgtgaaca ttattcgctg
     2521 accagcgatg cgccatcggg gaaggaaggt gccgcattca tggcgctgat ggcagaaaaa
     2581 gcccgtcttg ctgccctgtt acctgtggga tggtcacggg acatgacgac attcctgtca
     2641 ctcagtcagg aggtgctgtt atccctgctc agtttctgca ccgcatgcag ccttaacggt
     2701 gtccagaccc gtgagtgtgg tcacacgtca cgcagtccgc ttgactcgct ggagagcgcc
     2761 atcggctttc acatgcgtga ctggtggcag ccgacgaaag caaacttctt cggacacctg
     2821 aaaaagccgc agattatcgc agccctgaat gatgccggac tgtccggtgc cgcacgggac
     2881 gcggagaaga tgaagaaagg cgatgccgct gaacatgcag agttccacat gaaagacaac
     2941 cgctgggtgc ctggctggat gggttcacca cgtccacaga cggatgccac tgaacgcgcc
     3001 gataacctgg ctgatgccgc ctgatgaaca accacaccgc cccgccggag acggggcggc
     3061 agcaagggag ataccgtgat gaaaactgaa ctgaccctgg atgtattaca gaccatgagc
     3121 gcacaggaat atgaagatat ccgggctgcc ggaagtggtg aacgtcgtga gctgacacac
     3181 gccgtgatgc gggagctgga tgcgccggat aactggacga tgaacggcga gtacggcagc
     3241 gagtttggcg gctttttccc cgtccaggtc cgtttcacgc ctgcccacga acgttttcac
     3301 ctggcgttat gttcaccggg tgacgtctct caggtctggg tgctggttct ggtgaatgcc
     3361 ggcggtgagc cgtttgcggt ggttcaggtg cagcggcggt ttgtaccgga agccgtcagc
     3421 cattcactgg cactggcggc gtcactggat gcgcaggggt acagcgtcag tgacatcatc
     3481 catatcctga tggcggaagg aggtcaggca tgagcgtacg ttcacaggca ctggttcccc
     3541 tcagcgcaga gcaacaggcc gcatggcggg cggtggcgga gacggaaaaa cgccgccatc
     3601 agggaaacac gctggcggaa tatccgtatg cgggggcgtt tttccgctgt ctgaacggaa
     3661 gccgctggat ttcgctgtct gacctgcgat ttttcatgcc gtccctgacc gcagaagaac
     3721 tgcacggcaa ccgtcttcag tggctgtatg ccatcgatgt gctgattgaa acacagggcg
     3781 aggtctgcct cctccccctt cccggcgatg cagcagaacg cttgttcccg tcggtgcggt
     3841 tccgtgtcag ggaacgccgt cgccataaat ctgcgctggt catgcagaag tacagccgcc
     3901 agcaggcgag ggaggcagag cagaaaaccc gtgcgtacca ggcgctggtt gctcaggcag
     3961 aaatcgaact ggcgttccat tcaccggaaa cagtcggaag ctggcatgca cgctggagtg
     4021 accgggtggc agagcatgac ctggaaaccc tgttctggca gtggggcgag cgttttccgt
     4081 cactggccgg aatggaacgc tggcagtggc aggatatgcc gttctggcag gtcatcgcag
     4141 aggcaggtat ggcggcaagg gaggccggtc atgccgtccg tgagatggag cgatggatgg
     4201 tgccgaacaa actccgggag gcggcgtgaa gtggcgttac tcactgcgct ggaaactgcc
     4261 ttatccctgt cctggcgagc atgaactggt ttcagaagtg gtggaggcgg ggcagcctgc
     4321 accggcatcc gttatgtcac gctgggttgc cggagcaggg tatgcggtct gtctggattt
     4381 tatttctgac cgcccggtgc gccgctggag cgaagaacgt aaggctgcgg tacggcggcg
     4441 gaatctggag aaacgtatca acaggcatgc accactgttt gcggatgaac tgattgcccg
     4501 tgaactggct gaacgaccgg attattttca gggaaagtga gtgtggtcag tacgcaggta
     4561 tatgggctat gatgtgcccg gcgcttgagg ctttctgcct catgacgtga aggtggtttg
     4621 ttatcctgtc gtgtggcaga aggacaaaag ccccgtagtt aatttttcat taacccacga
     4681 ggcctctgca tgtctagtcc acgtcagtat ggcctcttac cgtgcaaatt gcaaggagga
     4741 gtaaaccatg aaactaccgg gaaacgccct tatctggtgc gtattaatcg tgtgctgcac
     4801 gttgttgata ttcacactcc tgacccggaa tcgcctgtgc gaagtccggc tgaaggacgg
     4861 atacagggaa gttacggcaa caatggctta cgaatccggc ggtaagtagc aacctggagg
     4921 cgggcgcagg cccgcctttt cagggctgat gctggtctga ctgcactgaa gcgcctttat
     4981 aaaggggctg ctggttcgcc ggtggcccct ttctccttgc tggcattgta gcagcatgaa
     5041 caaccagact gtcaggaagg ccacttccct ccgggaacgg gaatgaacgt gcgcctgcgg
     5101 cgctcgttct tttcccccgc cttctctgtt tatggccagt tgtcagaaat gacattacct
     5161 ttcccgtgat tattctcttt ttcttcctgc tctgattctg actactgcag tgttgtcctg
     5221 tctgccgggc gcaccttcgg ctcccggctg cctggcggca tccggtcgtc aggcccgact
     5281 ccgacaggca gctgctggcg cacctgccag tctccgccga acccgctggc gcggtttcgg
     5341 gctgccgcta cggtccgatg cgtgaaaacc ccgggcttgc agcgaccgtt ccggtcgcgc
     5401 cgcctctgtc gcgccgtggc ctgcgctccc ggcatcccgg caaaaagacg ccgggatgta
     5461 aggcgcaacg ccgtttcgca aaccgcgctt cacgtcgttg cggcgggtcc ctgcccgttg
     5521 ttgtgtccgg tgccattcac ctgtttaagc ccctgtcgcc gggggctgat acagccacaa
     5581 cctgccgcac tgactgtcca gggtaaatga acagcccaca aaatttttta cccaaaaccc
     5641 ggt
//
LOCUS       DAZGBC010000246         5580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGBC010000246 DAZGBC010000000
VERSION     DAZGBC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.248"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..329
                     /locus_tag="ACHK1J_004739"
                     /pseudo
     CDS             1..329
                     /locus_tag="ACHK1J_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            339..530
                     /locus_tag="ACHK1J_004740"
     CDS             339..530
                     /locus_tag="ACHK1J_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913902.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
     gene            1105..2661
                     /locus_tag="ACHK1J_004741"
     CDS             1105..2661
                     /locus_tag="ACHK1J_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO7913903.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2711..3430
                     /locus_tag="ACHK1J_004742"
     CDS             2711..3430
                     /locus_tag="ACHK1J_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO7913904.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            3441..4856
                     /locus_tag="ACHK1J_004743"
     CDS             3441..4856
                     /locus_tag="ACHK1J_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO7913905.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            4861..5556
                     /locus_tag="ACHK1J_004744"
     CDS             4861..5556
                     /locus_tag="ACHK1J_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO7913906.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
BASE COUNT         1482 a         1252 c         1354 g         1492 t
ORIGIN      
        1 tatggaacat ttgctttctg gctaacggca tgcccgacac atggttttac accgatgacg
       61 cctccgtcac aaaagagtgg gggcgggaat attgtgacaa tgtggaagtg ttccctctgt
      121 gctatatgca tgacggtccc gttttccggg ggcgctatat tgatgagctc agcgccgcac
      181 ggaacagaat gctccatatg caccacatac tgccggaatg aacccggtac agagaaaatt
      241 ttcatgacgc agaatgtatc cgtgaaattc acgaagcgcc cctgcaggct cccggcattc
      301 tgcaaaaata caaacgcaca aaatcataag actgttctat gtggatactg attttctggc
      361 tctcatcccc ctttaatgtc tcctccggcg atgcccggca acccacactg gccgttcaca
      421 gtcaggagtt ccgtactgaa aaagcctgtc gggatgcctt cagcgcgctg aaaaaagaaa
      481 acgtgggaga attcgctctc cggggcgtct gcgtccccgg cggagaataa accggagatg
      541 atgattctcc acaggagtat tcaatggcat accattcccc ggatgtcaca ctgcattatc
      601 cgtatctgcg ccggatgtcc tgacacgtct gcctgaatgg ccggaagagc gcttgcacga
      661 cttactaccg ttctcagcct accgattcgc agaattaact gacgttacgg tcataccacc
      721 taatgcaggg aaacgagtca tggaggtcgt cagctctact cccatgaatg acacagcctc
      781 tgaagtaaaa ataccgacgc agtgaaacca gccttaagct ttccaaaaca caattacaac
      841 atagttgaac aaagttcatt caaacaggtt cttttaataa catttagtca aataaaataa
      901 caataaaacg attcagcaat catttttcat aacaaaaaca aattataacc cccttccctg
      961 tttgtggacg agactcgttc tctccctgcg agacaagcgt aaattggtca caccgacggt
     1021 agttgcctga aaattacaaa ttctcgtctg acagggcaat aacaacagta atgatgctga
     1081 atttgttttc gttgaggaaa tgacatgcac caatacctgc tttttttact gggacttacc
     1141 ccacttctgg caatgatcat tttgattctg aaagtcaaaa tgcccataca ttatgccgta
     1201 ctcagcacac tctttattac actgattctt gcaactttgt tctggcacac accagttcag
     1261 gatctgggtt ctgctgttgt atatggcgca atcaaagggc tgtggccgat agtgatagtc
     1321 atacttgctg ccatttacag ctataacctg atgaatgcga caaaaagcat gaacattctg
     1381 cgaaatgttc tggccgctgt cagtgatgat aaacgtatcc aggtgcttct tatctcatgg
     1441 tgttttggtg gatttcttga ggcggcggcc gggtacggta cagctgtagc aatccccatt
     1501 ggtatactca ttgcactggg ctttaaccct cttaaagccg caattgcatc attggttgct
     1561 aatacagtac ctaccgcgtt tggtgctgtc ggcatacctg tatcagtact tgctgaacag
     1621 gtaaatttac ccgtaacaac cctcagcggg acgattattc ttcagctggc agcgttcaac
     1681 attctgcttc ccttcgttat agtcgctatt attggggaag gcctgaaggc tatacgaggc
     1741 gttgggttca tgaccttaat gtgcggtgtg agcacattaa tcccacagta cctggtggct
     1801 gtttatcttg gcgcagaact tcctgctttc gcgggaagtc tggtcagcct gatcgtgata
     1861 gtctttatgg caaaaaaccg taaagaaaaa acagctcctg aatttcttat tgagacttca
     1921 gcagatagcg ccagcgcact ttcatactct cgtggggcgt tgttacgtgc gtgctcaatc
     1981 tacatcctga tttttctgtt cattcttctt tgctctccac tgttcccgac ggtaaaaagt
     2041 acagtgtctc agattgcctc gtcactcagc ttctctctga gtaacggcag caatctgtcg
     2101 atgaagattg aatggatcgc aacccccggt gttctgatta tcattgccag tgttattggc
     2161 ggcttgatac agaaagcatc cctgtcatgc ctgttaaatg tatttgtcag tacggtaaaa
     2221 cagctcaaaa actccattat tgccatttct gcgattgttg ctatggcaac ggtaatggat
     2281 gtttgtgggt tgattggcac tctggcacag agccttgtag atctcactgg tggggcctac
     2341 ctcttcattg ctccggttat cggcgctctt ggtacatttg ttactggcag tgacacaaac
     2401 tccaacattc tgtttggtaa attgcagacc actgcggctg aaaaattgca tgtggaccca
     2461 ctctggctgg ctgccgctaa cacatctggc gcaacaggcg gcaaaatgat ttctcctcag
     2521 agtattgcta tcgctgtttc agccacccgt atggatggtc aggggagcgt aatcatgtca
     2581 gggacactga agtactgctt tgcttatatt gttattcttg gactgaaagt aggccttgtc
     2641 taccacctgt tcatggcctg agaaaaaata acaaacggac gtggaatatt aatcaacagg
     2701 agaaatatct gtgaatgtta actttttcgt cacctgcatt ggtgatgcat ttaaatcccg
     2761 tatggcacaa aacagcgtgc tgttactgga gcaattgggt tgcaatgtcc atttccccga
     2821 aaaacaggga tgttgcggtc agccagcgat aaatggcggt tatgttcgtg atgccattcc
     2881 tggtatgaaa aatctcatat cttcgctgga agagaatgac tggccggtag tatctcctgc
     2941 cgggtcatgc acttacacga taaaaaatta cgctcgctat ctcagtgaag aacctgagtg
     3001 ggcggcacga gctgaaaaag tagctgaacg cctgcatgat ttgacgtcat ttatagtaaa
     3061 tcatcttggc atcacagatg ttggggcaag actcaatggt cgcgcagtgt accatccttc
     3121 atgcagcctt tcccgtaaac tgggtgtaac ccgagaaccg cttttgttgc ttgagaatgt
     3181 acggggactt gagctgctgc cgtttcacgc tcaggaaacg tgctgcgggt ttggcggcac
     3241 gttctctgta aaaatggcgg agatctctgg tgaaatggtg aaagagaaag tcgggcatat
     3301 tatggatgtt aaacctgatt ttcttatcgg ggctgacgcc agttgcctgc tcaacatcgg
     3361 cggtcggctt agccgtgaag ggcatcctgt aaaagtgatg catattgctg aagtcctgat
     3421 gcacagatga ggtaaacaga atgtctttga aaacaagtga tatcaggttt aaagagcgta
     3481 tccgtattca gattcaggac ccgatcatgc gaaaagctgt ggctaacgca caggagcgca
     3541 tagggactaa tcgacagaaa atggttgatg aactggggca ctgggaagaa tggcgttcac
     3601 ttgctgaaca gattcgtcgg catgttctgg aaaatcttga tgcttatttg tatcaattgt
     3661 ccgaaaaagt tactgcgaat ggcggacatg tatattttgc ccgtaccaga gaggaggcaa
     3721 ccagttacat actgaaggtg gcgaaagaaa aaaacgccag gaaggttgtt aaatccaaat
     3781 caatggtgac tgaggaaatt ggtatgaacg gcgttcttca gaaagccggg ataccagtga
     3841 tagaaacaga tcttggtgaa tatattcttc agctggcgca tgagccgcca tcgcatgttg
     3901 ttgtgccggc aatacataaa gatcgccatc aaattcgcag agttttgcat gaacatctgg
     3961 ggtatgacgg ccctgaaact ccagaagcaa tgacattgtt catccgtgag aaaatcagac
     4021 aggattttct gagtgctgaa gttggcgtaa cagggtgtaa ctttgccgtt gcagaaacag
     4081 gctcagtttg ccttgtgaca aacgaaggta acgcacgtat gtgcaccacg ttacctaaaa
     4141 cacatattgc tgtgatgggt atggagcgta ttgcaccaac attccgggag gtggatgttc
     4201 ttatcaccat gcttgcaagg agtgctgtgg gagcaagact gacaggttat aacacctggc
     4261 ttacaggtcc aagagaagaa ggacatgttg acggcccgga agagttccat ctcgtcattg
     4321 ttgacaatgg gcgttcagag gttctcggct cagaattcag cgatgtattg cgctgtattc
     4381 gctgcggtgc ctgtattaat acctgtcccg cttatcgcca gattggtgga catggctatg
     4441 gctcaatcta cccgggccca atcggagcag ttatttcccc gttgcttggt ggatatgaag
     4501 actttaagga attaccttat gcctgctcat tgtgcactgc ctgcaatggg gtatgtccgg
     4561 taaaaatacc tctggcacaa ctaatacaaa aacatcggca ggttatggca gagtccgaaa
     4621 taacaccatc aacagaacgc cgtataacca gaatattcaa ttacgtaaac agtcatccgg
     4681 cattgtggaa agttggcatg attgctggcg cgaaaacagc cagcctgtgc atcaggaacg
     4741 gaaaaacgcc gattagccca ggtgctatta aagaatggtc tgtggccaga gacttacctt
     4801 ctcctgatgg agaaagcttc cgtagttggt tcaaaagaca taaaaaagga gggtaacttt
     4861 atggaaaaca gagaggaatt tctcgttgct atcgccagaa agcttggcag gccagtaaga
     4921 cgtattcccg aagcaatgcc ggagcctgta agtacgctgg ctacgacacg tttaaccgag
     4981 ttgacaatgg accaacgctg tgaggctttt attaaatttg cctcagaagt gatgcttgcg
     5041 aagtgcgttc tggtttcgcc tgaagacgca ccatcaaaag cattagacat ctgttggacg
     5101 ttcggttccg ggccagtcat tatcagcaac gatcaacgac tgtttgatac ggggattgca
     5161 ccattgctac aaaaagagat gggcgctgtc ctgtggaccc cggagaaggg tgaggaaaat
     5221 attcatcttg cagaaaaagc aaaaacaggc gtcgttttcg ctgagtatgg actgacagaa
     5281 tctggtggtg ttgttttatt ctccgcccct gaacgagggc gttcagtaag tcttttacct
     5341 gaagcgtctg tgtttgtttt acgcaaaagt accattcttc ccagagtggc tcagcttgcg
     5401 caaaaacttc atgatatggc tcaggaagga caacgtatgc cttcctgtat caatctgatt
     5461 ggaggcccaa gttctactgc agatatagaa ctgatcaaag ttgttggtgt gcatggacct
     5521 gtacatgcaa cttacttgat tattgatgac tgctaataca ttttagtatg tatatatgat
//
LOCUS       DAZGBC010000247         5376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGBC010000247 DAZGBC010000000
VERSION     DAZGBC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.249"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1110
                     /locus_tag="ACHK1J_004745"
     CDS             <1..1110
                     /locus_tag="ACHK1J_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7913907.1"
                     /translation="FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRV
                     RVKFNWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIM
                     GRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTE
                     VLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVG
                     SNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNN
                     VTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVN
                     GDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1186..>5376
                     /locus_tag="ACHK1J_004746"
     CDS             1186..>5376
                     /locus_tag="ACHK1J_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFO7913908.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSSFSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTYPDGLRSSREYDEKGRLAAETSRSGETTSYSYDDPASEL
                     PTGIEDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRYGQRTAVHQEEGLSIYSLY
                     DSRGRLTGQRDAQGRETRLEYSAAGDLTVIIAPDGSRSETQYDAWGKAISTTQGGLTR
                     SMEYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYHYDLTGKLTQSEDE
                     GLITLWHYDASDRITHRTVNSDPAEQWQYDEHGWLTTLSHTSEGHRVSVHYGYDDKGR
                     LTDERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKL
                     GGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTLTGQLQSQHLNLPQLDRDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHR
                     LVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTIQTGTTRIQTVYEPGSFTPLIRIETENGEQAKARHRSLAEVLQEDTGVTLPAEL
                     SVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLHLYHCDHRGL
                     PQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGLYYNRNRYYD
                     PLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDILFTGGSGPDN
                     KILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRPSRTAEEEVQ
                     AQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWPGKHADEIKN
                     VKASLKNTLIDVKKRCMPT"
BASE COUNT         1278 a         1368 c         1818 g          912 t
ORIGIN      
        1 tttgcggtaa tcccggcaga ccggacatgg cggccacagc cgttgctgaa accgctggtg
       61 gacggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt ctgtgatgaa
      121 catggccgcg tgcgggtgaa atttaactgg gaccgctata acccgtcaaa ccaggaaagt
      181 tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gctttggtaa cctggcgata
      241 ccgcgtgtgg gtcaggaggt cattgtggac ttcctcaacg gcgatccgga ccagccgatc
      301 attatggggc gtacctacca ccaggaaaac cgcacccccg gcagcctgcc ggggacgaag
      361 acgcagatga cgatccgctc gaaaacctat aagggcagcg ggtttaatga actgaagttt
      421 gacgatgcga cagggaaaga acaggtctac atccacgcgc agaagaacat gaacaccgag
      481 gtgctgaata accgcaccac tgatgtgata aacaaccatg ccgaaaaaat aggtaacaac
      541 caggcgatca ccgttaccaa taaccagatc cagaacattg gcgttaatca gatacagacg
      601 gttggtgtca accaggtgga aacggtgggc agtaaccaga ttatcaaagt gggatcaaac
      661 caggttgaaa aggtggggat cattcgtgcg ctgacggtgg gtgtggctta ccagacgacg
      721 gtaggcggca ttatgaatac ctcggtggcg ttgttgcagt cctcacaggt agggctgcat
      781 aaatcactga tggtgggaat gggctacagc gtcaatgtgg ggaataacgt caccttctcg
      841 gtgggcaaga caatgaagga aaacaccgga caaacagcag tttattctgc cggtgagcat
      901 cttgaactct gctgtggtaa ggcaaggctg gtgctgacga aggacggaag catatttctc
      961 aatggtacgc acattcatct ggaaggggag tcggatgtga acggtgatgc gccagtgatt
     1021 aactggaact gtggtgccac acaacctgta ccggatgcgc ctgtgccgaa agatttaccc
     1081 cccggaatgc cggatatgcg gcaattttga gtcaggtgca aaaaattaaa acggaactgg
     1141 tgcagtgacc agataaacca tattaaaagc agaaggaatt gattcatggg cggaaaaccg
     1201 gcagcgcggc agggtgatat gacccgcaag ggactggata ttgtgcaggg ttcagcgggg
     1261 gtgctgatag gtgcgccgac gggagtggcc tgttcggtat gtccgggagg gattacctat
     1321 gctaacccgg tgaacccgct gctgggtgcg aaggtgctgc cgggcgagac ggaccttgcg
     1381 ctgcccggcc cgctgccgtt tattctttcc cgcgcctaca gcagctaccg gaccagaaca
     1441 cccgcgccgg tgggggtgtt tggtcccggc tggaaagcgc ccttcgatat ccgcttacag
     1501 atacgcgatg aaggcctgat actcaacgac aacggcggca ggagcattca ctttgagccg
     1561 ctgtttcccg gcgagataag ctacagccgc agcgagtcac tctggctggc gcggggcggg
     1621 gtggcggagc agcacagttc gcagccgcta agcgcactct ggcaggtact gccggaagat
     1681 gttcgcctga gtccgcatgt gtacctggcg acaaacagcc ttcaggggcc gtggtggata
     1741 ctcagctggc cggagcgggt gccgggggcg gacgaggtgc tgccgccgga gccgcccgca
     1801 taccgggtgc tgacgggcgt ggtggacggc ttcgggcgga cgctggcctt tcaccgggcg
     1861 gcggagggtg atgtggcggg cgcggtgacg ggggtgacgg acggcgcggg ccgccgtttt
     1921 cacctggtgc tgaccacaca ggcacagcgg gcggaagtat tccgtaaaca gcgcgccacg
     1981 tctttatctt cccctgccgg tccccgttct gcttcctctt cttccttttc ttcagctttc
     2041 cccgacacgc tgcccgccgg tacagagtac ggtgccgata acggtatccg gctggaggcg
     2101 gtgtggctga cacacgaccc ggcatacccg gatgaacagc cgactgcgcc gctggcgcgc
     2161 tacacgtaca cggccagcgg agaactgcgg gcggtgtatg accgcagcgg gacgcaggtg
     2221 cgcgggtttg cttatgatgc ggagcacgcc gggcggatgg tggcgcacca ttatgcgggg
     2281 aggccggaga gccgctaccg gtatgatgat accggccggg tgacggagca ggtcaacccg
     2341 gaggggctgg actaccgctt tgagtacggg caggaccgtg tgaccatcac ggacagcctg
     2401 aaccggcggg aggtgctgta cacggagggc gagggcggcc tgaagcgggt ggtgaagaag
     2461 gagcatgcgg acgggagcat tacccgcagc gagtatgatg aggcggggag gctgaaggca
     2521 cagacggatg cggcggggcg gcggacggag tacagcctgc atatggcgtc gggtgcggtg
     2581 acggcggtga cgtacccgga cgggcttcgc agcagccggg agtatgatga gaagggaagg
     2641 ctggcggcgg agacctcacg cagcggtgag acgacgagtt acagctatga tgacccggcg
     2701 agtgagctgc cgacagggat agaggatgcg acgggcagta caaaacagat ggcatggagc
     2761 cgttacggtc agctgctggc cttcacggac tgctcggggt acacaacgcg gtatgaatac
     2821 gaccggtacg gtcagaggac ggcggttcac caggaagaag gcctcagtat ttacagtctc
     2881 tatgacagcc gcggcaggct gacagggcag agggatgcgc aggggcggga gacgcggctg
     2941 gagtacagtg ccgcaggcga cctcaccgtt atcattgccc cggacggcag ccgcagtgag
     3001 acacagtatg atgcgtgggg aaaggccatc agcaccacgc agggcgggct gacgcgcagc
     3061 atggagtatg acgctgccgg gcgcatcacc gtgctgacca acgagaacgg cagccagtcc
     3121 acgttccggt atgacccggt ggacaggctg actgaacagc gcggttttga cggccggacg
     3181 caacgttacc actatgacct gaccggaaaa ctcacgcaga gtgaagacga ggggcttatc
     3241 accctctggc actacgatgc gtcggaccgc atcacgcacc ggacggtgaa cagcgacccg
     3301 gcagagcagt ggcagtatga tgagcacggg tggttaacca ccctcagcca taccagtgaa
     3361 ggccaccgcg tgtcggtcca ctacggctat gacgataaag gccgcctgac ggatgaacgg
     3421 cagacggtgg agaacccgga gacgggggag atgctgtggg agcatgagac ggggcatgcg
     3481 tacagcgaac aggggctggc gacccgtcag gagccggacg gtctgccgcc ggtggagtgg
     3541 ctgacgtatg gcagcggtta tcttgcgggg atgaagctgg gcggaacacc gctggtcgag
     3601 tacacgcggg accggctgca ccgtgagacg gtgcgcagct tcggcagcat ggcaggcagt
     3661 aatgcagcat atgaactgac cagcacatac accctcacag gccagctcca gagccagcac
     3721 ctgaacctcc cgcagcttga ccgtgactac gactggaacg acaacggaca gctaatccgc
     3781 atcagcggcc cgcaggagag ccgggagtac cgttacagtg acacgggaag gctgacgggc
     3841 gtccacacca cggcagcgaa cctggatatc gatatcccgt atgcgacgga cccggcagga
     3901 aaccggctgc cggacccgga actgcacccg gacagcacgc tcacggcgtg gccggataac
     3961 cgcatcgcgg aagatgcgca ctatgtctac cgccacgatg aatacggcag gctggcggag
     4021 aagacggacc gcatcccgga aggggtgata cggatgcacg acgagcggac ccaccactac
     4081 cactacgaca gccagcaccg tctggtgttc tacacgcgga tacagcacgg tgagccgcag
     4141 gtggagagcc gctacctcta tgacccgctg ggccgccgga cgggaaaacg ggtgtggcgg
     4201 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacatggtac
     4261 gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat ccagacggta
     4321 tacgagccgg ggagcttcac accgctcatc agaatcgaaa cagagaatgg tgaacaggcg
     4381 aaggcgcggc accgtagcct ggcggaggtg ttgcaggagg acacgggtgt gacgctaccg
     4441 gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcggcaggg cagcgtgagt
     4501 gaagaaagcc agcagtggct tgcgcagtgc gggctgacgg cggaacagat ggccgcgcag
     4561 ctggaggcgg aatatatccc ggagcggaaa cttcatcttt accactgcga ccaccgggga
     4621 ctgccgcagg cgctcatcag cccggaaggc gaaacggcgt ggcaggggga gtatgacgag
     4681 tggggaaacc tgctgggcga aaccagcgcg cagcacctgc aacagccgta ccgtctgccg
     4741 ggacagcagt atgatgagga gtcggggctg tattacaacc gcaaccggta ctatgacccg
     4801 ctacagggga ggtatatcac ccaggatcca atagatataa aaggaggatg gaatttatat
     4861 tcttatgcgc ttaatccggt aagttggatt gacccattag gattgacgca gtgcgattcc
     4921 gaagggtgta ataatgatat attatttaca ggaggaagtg gtcccgataa taaaatactt
     4981 aatgaattag gtccgagaga tggcattgac ggtttgggtt cacaaaacat gaaaatgtat
     5041 agtgggctat tagggggcga tattttaaaa ccggggattc tgggcggttt gacgcttggt
     5101 agtgtgacac aacgaccatc gcgaacggct gaggaggagg tgcaagctca aatagaatat
     5161 ttagcatata agaagcgttg tgagcaaaaa cctgatagta atttaagtcg ttgtgctgcc
     5221 gcgatctttc aaaagagcag aaaagaagat tgtttaagaa tgagacaaga atgggatgat
     5281 aaatggtggc ctggcaaaca tgcggatgaa ataaaaaatg tgaaagcatc gttgaaaaac
     5341 acacttattg atgttaaaaa aagatgcatg ccaact
//
LOCUS       DAZGBC010000248         5293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGBC010000248 DAZGBC010000000
VERSION     DAZGBC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.250"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            271..1122
                     /locus_tag="ACHK1J_004747"
     CDS             271..1122
                     /locus_tag="ACHK1J_004747"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO7913909.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1149..2138
                     /locus_tag="ACHK1J_004748"
     CDS             1149..2138
                     /locus_tag="ACHK1J_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO7913910.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2169..3062)
                     /locus_tag="ACHK1J_004749"
     CDS             complement(2169..3062)
                     /locus_tag="ACHK1J_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7913911.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINIRLPTAGGLYHWEFEREGKALRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYITSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3263..4189)
                     /locus_tag="ACHK1J_004750"
     CDS             complement(3263..4189)
                     /locus_tag="ACHK1J_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7913912.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            4346..5266
                     /locus_tag="ACHK1J_004751"
     CDS             4346..5266
                     /locus_tag="ACHK1J_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7913913.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT         1444 a         1286 c         1212 g         1351 t
ORIGIN      
        1 gagtgaataa taatgaccta cctttaatta gcaactattg gaaatacgtc gaagtaggta
       61 tatacgccag gataaaccga tatatttttt atggatattc attttccttc atcggactgt
      121 aaattgcgga gatatcgaac ttgcactacc catcgcaaca ctcaaacaga tttatgaatt
      181 tcgttcataa cccctgacaa atcctgccgt ctaatcgagc ttttcccttt acgttatgct
      241 tctcatgaac gagaacggaa ggataacacc atgcaaaaag taaaactgaa caacggtatt
      301 gaaatgcccc tgttgggctt tggtgtattt cagatgaccg atgccgctga atgcgaaaga
      361 gcggtcattg acgctattaa caccggttat cgactgattg acaccgccgc ctcctatcaa
      421 aatgaaaccc aggtcgggaa cgcactgaaa caaagcggta tcgcccgtaa cgaactcttt
      481 gtcaccacga aactctggtt gcaggatacc agttacgaag gggccaaagc ccagttcgaa
      541 cgctctctga atcgactgca acttgattac gttgacctgt atttgattca tcagccttac
      601 ggtgatgtcc acggtgcctg gcgtgccatg gaggagctgc aacaggccgg taaaattcgc
      661 gctattggcg tcagtaactt ccatcctgac cgactggctg accttatcgc ctttaataac
      721 gtcgttccag cggtaaacca ggttgaagtt aaccctttca atcagcagct acacgccgtt
      781 ccgtggatgc aaagccgtgg tattcagcca gaagcctggg caccgtttgc ggaagggaaa
      841 aatggtctgt tccagcatcc tgttctcacg gcaattggcg agaagtatgg caaaagcgta
      901 ggccaggttg ttctgcgctg gatctaccaa cgaggcattg tttcgcttgc aaaatcagta
      961 cgcaaagaac gcatggaaga gaacatcaac attctcgatt ttgaactcag tcctgaagat
     1021 atgctgcaaa ttaccgccct ggataccgca accagcgcgt tcttctctca ccgcgatccc
     1081 gcaatggtgg aatggctgac tggccgcaaa cttgatgttt aagccgcgat aaaagaggta
     1141 ttcattcaat gcaaaaacgt tatctgggta aatccggact cgaagtttcc gcccttgggc
     1201 tcggttgcat gggcttaagc cacggctacg gcccggcgac cgatacccgt caggctatcg
     1261 aacttattcg tgctgcggtt gaacgtggcg tcaccttctt cgataccgcc gaagtgtatg
     1321 gtccgtatct gaatgaagaa gtggtcggcg aagccttaaa accgtttcgt gaccgcgtgg
     1381 tgatcgccac taaattcggt tttaccttcg gcgatgataa taagcagcag attttaaaca
     1441 gccgtccgga acatatccgt gaagccgttg aaggatcatt acgtcgtctg aagacagatg
     1501 ttattgacct gctgtaccaa caccgtgttg atccggatgt tcctattgaa gatgttgcag
     1561 gaacggtgaa agacctcatc gcagaaggta aggtcaaaca tttcggtctg tccgaggctg
     1621 gcgcacaaac cattcgtcgt gcgcatgcgg tacaacctgt caccgccctg caaagcgaat
     1681 attccatgtg gtggcgtgag cctgaacagg aaattctgcc gttgctggag gaactgggga
     1741 ttggttttgt ccccttcagc ccactaggta aaggtttcct gaccgggtcg attaagccgg
     1801 gaaccacttt cggcaaagat gattaccgca gcaccgtacc gcgtttcgca gcacaagcca
     1861 ttgaagctaa tgaaaaactg gtcaccttgt taggtgaact ggctgcggag aaaggggtaa
     1921 cgtctgcaca aatcgcactg gcatggctgt tagcacaaaa gccgtggatt gttcccatcc
     1981 ctggcaccac taaactgcac cggctggaag aaaacctggc cgctgctgac attgttcttt
     2041 cgcagaaaga tacgcagcag ataaccgagg cgctggaaac aattaaaatc gtcggtgaac
     2101 gttactcgcc tgaacaccag gcacgcgtag gccgctaata cccacacggg tcctggacaa
     2161 aggacccgtt attcggtgta gcgaagcgca tcgatcaaca gcgcaaaagc aggcggatgc
     2221 tgcttgcggc tggggtagta gagataatat ccgggaaaag ttggacacca ctcctgcaac
     2281 acctgaataa gcttgcctga cgttatatac tcctgaacca tgtcttcagg gacacaagcg
     2341 atcccaaacc ctgataacgc ggcatcaatt ctttctggca atggactaca cgtcagctgc
     2401 ccatcaacct taacgcgaag cgctttccct tctcgctcga attcccagtg gtaaagtcca
     2461 ccggcagttg gcaaacgtat gttgatgcag cgatggtttt gtagctcgtg aggcgtttca
     2521 ggcgcagggt ttgcagcaaa atacgccggt gcccccacca cagccatccg catatctggt
     2581 ccaattctga ccgcaaccat atctttatca acattttcac ccagtcgtat cccggcatca
     2641 aaacggcctt caacaatatc gacaaaaccg ttatccacca ccagttcgac actgatttcg
     2701 ggatactttc tgagaaaagg ttttagcttc ggccagacca gacttcgcgc ggcatgttcc
     2761 ccggcagata aacgaatatt cccggagggc gtgccgttct gttgaataag tgactccagt
     2821 tcctgttcaa gctcgtcaat acgcggttca aggcaggcaa tgattctctc ccccgcttct
     2881 gtgggagcca cactgcgggt agtccgtgtc aggaggcgga tattgagcct ttcctccaga
     2941 gccttcattg catggctaag tgcagactga gagacgccga gtttgcccgc tgctttggta
     3001 aaacttcgct ctcttgccac cacaagaaag atttgcagtt cgttgaagtt ttctttgagc
     3061 attggcgatt tccttgtaga aacatcctct tcacagacac tcatgaaggg ggggcataga
     3121 tgctattatt ttagccacat tattagtaac aatgataatc tggctgtatc gaaaataatt
     3181 ctaaactttc agaagattta ttatgaataa acgtatcctc acgattgtgc cattcttctt
     3241 accgatattc tgacacatat catcaatttt cgagttttac tcttttctcc accataaaat
     3301 caataaaagc acgaacttta gagggcattc tcacccgtga tgaaacataa atataaaatc
     3361 caggaaatga aacagcccaa tcctcaagaa tcggctgtaa cttgcccgca ttgatatgtt
     3421 cagcgattgc aaaatccaga tgttgaatga tacccgttcc attcagcgcc attcgtatca
     3481 tgctttcgtc atcattagaa atatacttac ctgaaacctc tatttcgaac tcttcattac
     3541 cccccacaac attagaaaac tcccatgcag agagtgcacc gctactttta tgccgcaacc
     3601 gaaggcaatt atggttactc agttcctgag gcgtttcagg aataccgtgt tcttttaaat
     3661 aatctggcga tgccacagta accagtttaa taagcggaga aacaggaacg gcagtcatat
     3721 gctcatccag tccttgctca agacgtatgc ccacatcaca gccgtccgca ataatgttag
     3781 ataaaccgtc atccataatg agttctatct ttgttttggg gtaacgagta agaaactcgc
     3841 caatatgagg ctcaacaagc atccgtgctg caacacggga ggaatttatt ctcaacaggc
     3901 ctgtaggttc aagttgggta tcaccaacat ctttcaaagc atcatcaatt tgccccaaag
     3961 ccgacaccag cacttcatac agacgctgtc cttcctcagt aagcgacata ttgcgggtcg
     4021 tacggtatat cagcttggtg ttaagacgtt cctcaagaga ttttaaattc tgggacaatg
     4081 cggcccgcgt aatacccatt tcagaagcgg ctctggaaaa actgccggca cgaacaatca
     4141 gcgcaaacca ggcaagagaa ggcaaaattg aaggctctat tttcatcatt gtaaacctta
     4201 acttaacact gtgatagata ttagaccatt gatgataacc aatacaagtc atactattac
     4261 gccgttctcg atatgaacac gaatgccagt tgcggataat agaaatgata tttaaatctg
     4321 tttaggcaaa tccccggagc ttgaaatgaa aactgtttcg ataaaacaca cttactggga
     4381 tatagctgct gatatctatt tccctgacga ttttaatagt gaaaaaaagt atcctgccat
     4441 tatcagcgcc catccaattg gaagctgcaa agaacagaca tcgggtagtg tgtatggtgc
     4501 cgccctggca aaagctggct ttatcgtcat tgcttttgat gccagttttc agggaagcag
     4561 cggtggggaa ccgcgttatc tggaagatcc aacaatgcga gtaaaagact tcagcattgt
     4621 cgttgattac cttaccagct taccttacgt tgatgccggg cgtattggtg tactgggtat
     4681 ttgtggtgga ggtggatatg ccattaatgc cgcaatgaca gaacgtcgaa ttaaagctat
     4741 tggtactgtc acgggtgcaa actacgggcg tcttatgcgt gagggattta cagcgtttaa
     4801 tcctatcgga gcactcgaag caatggcgca acaaagaaca gacgaagcta acggcgcaaa
     4861 acttcgcgta gatgatttac tcccctcttc tccggatgca gctcttgaag cgggattaac
     4921 agaaatagac ctttttgaag ccacagaata ttatcgttcg ccacgaggct atgcacctaa
     4981 tggggtaaat cgttcgctat tctcacatca gactgttgcc gtgggttggg atgcatttca
     5041 tcttgcagaa gtcctgttaa ctcaaccaat aatggttgtg gtgggcgata gagtgggcgc
     5101 atttggtgcc taccgtgacg gctgcgaaat tattggccgc gctgcttcaa aacataaaga
     5161 gctggtcgtg gttgaaggtt attcacatta cgacctgtat gataagccag aacctgtgaa
     5221 acaggcactc gataaattaa ttccttttta taaaacacat ttgtaataat cttacaggga
     5281 gattccttat ctc
//
LOCUS       DAZGBC010000249         5255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGBC010000249 DAZGBC010000000
VERSION     DAZGBC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.251"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..204
                     /locus_tag="ACHK1J_004752"
     CDS             <1..204
                     /locus_tag="ACHK1J_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO7913914.1"
                     /translation="RMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVL
                     LTPGHYLGNWDHRLRHLQEQNVA"
     gene            complement(438..990)
                     /locus_tag="ACHK1J_004753"
                     /pseudo
     CDS             complement(438..990)
                     /locus_tag="ACHK1J_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1107..2012
                     /locus_tag="ACHK1J_004754"
     CDS             1107..2012
                     /locus_tag="ACHK1J_004754"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO7913915.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGILNTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKK"
     gene            2057..2932
                     /locus_tag="ACHK1J_004755"
     CDS             2057..2932
                     /locus_tag="ACHK1J_004755"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO7913916.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            2981..4453
                     /locus_tag="ACHK1J_004756"
     CDS             2981..4453
                     /locus_tag="ACHK1J_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7913917.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAVLITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            4457..>5255
                     /locus_tag="ACHK1J_004757"
     CDS             4457..>5255
                     /locus_tag="ACHK1J_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7913918.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEESSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLL"
BASE COUNT         1399 a          892 c         1316 g         1648 t
ORIGIN      
        1 aggatgtggg tgtcggcaac ggatattgac ccgctggcag ccggcatggc gtatatacaa
       61 ctgtcgctgt gtggtgtggc cggagaagtg gtgattggta actcgctctg taatgagcga
      121 cgccgcgtac tgctgactcc cgggcattac ctgggaaact gggaccatcg tctgcgtcat
      181 cttcaggagc agaatgtggc atgatgcaga atgttttatc actgcgccag tcttcggact
      241 ggcgttttgt tcaactgcca gttccctgaa tgttttattt gttcaataaa gcctgtttat
      301 caataaattt tctgacgaca gggaatggta tgtttttatt agtttttatg tgttggacaa
      361 caagacagaa taatgatgtc tgttttatgt atacgatata gccactgatt caacgcatta
      421 tcggctatat tgccagtctt atttagtgcc agattcattg tatcgaaggc cgctgccgtc
      481 agtagatggc tggtcagttc tatcagacag accattttta cctgaggata aagtgtctgg
      541 tttccaccat gtatttggcg agtgaagctt tatcgtgttc ttgcgtgtcc tgtgttcgtc
      601 agaacacgcc atcaatggtc agcagattca gatcgcacca gagcggatat ggcgtggtgt
      661 tatgccagag ctgcgctgtt tgagtgaaca cgcagcgtat ggctccgctt cctggccgct
      721 ggcgggcatg gagcacggcg ctactgcctg gcagtatgat gtccagccgg ttcacaatct
      781 gatgaaggtt ccttaagctc atgggccata ctgataattc accagaccat catttccaga
      841 ggaagacgcc gtttgtgtag cgtgacagtg tcggactctt cagggctgtg ggtaatcagt
      901 tccagggcaa gatagtcgca gagggttacg cagagaatag taactggaca ctggatcgat
      961 caaccgatcc ttaactgagc ggcatgacag ctttctgatg gatttttttg ataatcttca
     1021 cattgtgctt cttttctgtt ggataatttt tgcacttggt ggtgtactag gtatcccatt
     1081 atgcagttaa gtgaaggtga ttaaaaatgc aatgtgaatt taaaggggtc atatcagcat
     1141 tacccactcc ttatgaccaa tcccagcaaa tcgatatgga gagtcttcga aaactgatcc
     1201 gttttaatat cgaacaaaat attaagggat tgtatgttgg aggctctact ggagaagcat
     1261 ttcttcagaa tgttgcagag cgagaaaaaa ttcttgaaac agtagccgat gaatcagatg
     1321 gaagactgac acttatagcg catgtgggcg gaatcagtac ggcagaaagc gaggtacttg
     1381 caaaagctgc aaaaaaatac ggatatcatg ctatatctgc tgttactcca ttttattatc
     1441 ctttttcatt tgaagagcat tgtatccatt acaggaagat tattgattct gcagatggac
     1501 ttccgatggt agtttataat ataccggctc ttagcggagt gcgtttttct ttagatcaga
     1561 taaatgagct tgtcacaata cccggggttt gtgcattaaa acagacatcc ggtgatttat
     1621 tccagatgga acaaatcaaa aggaatcatc cagagcttgt gttatataat ggttacgatg
     1681 aaatatttgc atcagggtta attgcaggtg cagatggggg aatcggtagt acatataata
     1741 taatgggatg gcgttatctt gaaatatttg aagcagtcaa aaacaatgat gtgattaagg
     1801 cgaaggaaat gcaggtggcc tgtaaccagg ttattgacac tcttattcag agtggtgtcc
     1861 tggcaggtat aaaaactctt ctttactata tggggattct aaatactcct gtatgcagaa
     1921 gtcctttttc tccggtaaag gaaaagaatc tggatgtatt atcaaaactt gcagagcgac
     1981 tgctcgaaga acatgacaga aataaaaaat gaaaataatc tgaataattg caagattgtt
     2041 atataggtga aaatgaatga taacactggc tgttgatata ggaggaacga aaatttcagc
     2101 ggcgttgata tctgatgacg gttcattttt gctgaaaaaa cagatatcaa cacctcatga
     2161 aagatgtcct gacgaaatga caggggcttt acgtctactg gtttctgaaa tgaagggaac
     2221 agcggagcga tttgcagtag catcaacagg gattattaat aatggagtat tgacagctct
     2281 gaatccagac aatcttggag ggctgaaaga gtatcctctg aaaaatataa tggaagatat
     2341 aactggttta aatggaagtg tgataaatga tgcccaggca gccgcatggg cagaatatac
     2401 agttttgcca aaagaaattt gtgatatggt ttttataacc gtttctacgg gggttggagg
     2461 gggaattgta gttaacagaa aattattaac aggtgtttct ggattagccg gacatgttgg
     2521 gcatatattg tccggagtaa cggatacaga atgtggatgt ggtcgtcggg gatgcgttga
     2581 agcggtttca tctggtcggg ccatcatggg ggcagcaaaa aatgaacttg caggatatag
     2641 caccaaatat atttttgaat tagcgagaca gggatataag gaagcagaat ttttgacaga
     2701 gcgttctgcc agtactattg ctgaattaat agtaagttta aaattactgc ttgattgtca
     2761 ggttgttgtt gtcggaggaa gtgttggact tgctgatggg tatattcaga aagtgtcgaa
     2821 acatctttcc atatattctg aaatttgtaa tgtaatgctg tttcccgcat atttcagaag
     2881 tgactctggt cttattggtg caactctttg ggaccgggat tgtattactt aaagtaatac
     2941 tccagtgtat tataaatccg ctattattgt gaggtttaat atggactcct gtattcagaa
     3001 aaagcaggtt tggtataagc atctttcacc cagacagtgg aagatattcg gtgcggcatg
     3061 gacaggttat ttacttgatg gttttgattt cgttctgata tctctggttc ttacagaggt
     3121 tcagcatgaa ttcggcttga cgacaataga agcggcaagc cttatttccg ctgcttttat
     3181 ttcccgatgg tttggggggc ttgctattgg cgctttatcg gacaagatgg ggcgcaggat
     3241 ggcaatggtt ttaagtatag tgctattctc tctcggaacc cttgcctgtg gtctggctcc
     3301 tggatatgca gtgatgttta ttgctcgcat tgttattggg cttggtatgg caggtgaata
     3361 tggctcaagt gtaacatatg ttatcgaaag ctggcctgtt catctcagaa acaaggcaag
     3421 tggtttttta atttctggat tttctattgg gggaggttta gctgcacagg tttatagcat
     3481 tgtagtacca ctatggggct ggcgttctct tttctttgtc ggcatgcttc caatattatt
     3541 tgctttttat cttcgcaaga atcttccgga gtctgatgac tggcagaaaa ggcaacaaga
     3601 gaataaaccc gtcagaacaa tggtggatat tctgtacaga gaaaagaata aatacataaa
     3661 tattcttctg tcttgtattg catttgcatg tctatatgtt tgtttttcag gcgtcactgc
     3721 aaacgctgtt ttaatcacag ttatggcatt gtgctgtgcg gctgttttta tcagttttat
     3781 atatcagggt atggggaaaa gatggccaac aggtattatg ttgatgcttg tggttatgtt
     3841 ctgtttcctc tacggatggc cactgcaggc ttttcttcct acatggctta aggttgatat
     3901 gcagtattca ccagaaactg ttgctctcat atttatgctg gcaggttttg gctctgcagc
     3961 tggaagttgt ataggtggtt ttatggggga ctggcttgga acccgaaaag catatgttat
     4021 atcacttctt atcggtcagc ttgttattat ccccgttttt cttgtggacc gggactatgt
     4081 atggttactt ggccttctga tctttactca gcaggttttt ggtcagggga ttggtgctct
     4141 ggtgccgaaa ataatcagtg gttattttaa tgtggagcag cgtgcggcag gactggggtt
     4201 tatttataat gtaggttcac ttggtggagc atgtgcgcct attcttggtg cagttgttgc
     4261 gtctcataca agtcttggta cagcaatgtg ttctcttgct tttatcttaa catttgttgt
     4321 tcttgtcctt attggttttg atatgccatc acgtgtacag agatggatcc atccagaagc
     4381 tgcgcttgaa tatgatactg tcgatggaaa acctttttat ggagcaagaa aaaagaatgt
     4441 tgcaggagaa taacggatgc tatcagggaa taaaaaaatg attcattaca gggagaatat
     4501 atattctctt gataattatg agcgttacat aactgacagt tcgctatttg agaatactga
     4561 cgatggcata atatatacag ggattgtgcc ccttattaca gttgttgagc ctgttgtatc
     4621 taatcaacat agtgtgctga ttatacctgg aggtggatac aggaggattg tttatgaaag
     4681 agagggactt gatgtaggca gatggatagc gggtcaggga tatacggcat ttattctttc
     4741 ttacagaatg ccctccgtta atggttatta ttcctttgcg gatgcgaaga atgccctgaa
     4801 ctttatttct tactgggccg gaatgtcgga gagaacattt aagagtgttg gatgtatggg
     4861 gttttcggca ggtggtcatc ttgctggata tattactcat ctccattatg aggaagagag
     4921 ttctgtgcgt cctgactgga tggctctgat atatccagtt atatctcttg acaggagtat
     4981 tgctcatcag ggatgccgga aacgtcttct ggatgcaggt gaaagtgaat attcagccag
     5041 tcttgaaaat agtgttacag agtatatgcc accagtcttt attgtgcatg caacagatga
     5101 taaggatgtt ccagttattc attctctgag atattttaac tctatccggg ataagggaca
     5161 tcatgcagaa gctcattttt ttgataaggg aggtcatgga tttggtgtat ccggaaaaaa
     5221 tggatatccg atagaaaaat ggcaaagcct gcttc
//
LOCUS       DAZGBC010000250         5228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGBC010000250 DAZGBC010000000
VERSION     DAZGBC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.252"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(229..1926)
                     /locus_tag="ACHK1J_004758"
     CDS             complement(229..1926)
                     /locus_tag="ACHK1J_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO7913919.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            complement(2022..3158)
                     /locus_tag="ACHK1J_004759"
     CDS             complement(2022..3158)
                     /locus_tag="ACHK1J_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7913920.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            3834..4042
                     /locus_tag="ACHK1J_004760"
                     /pseudo
     CDS             3834..4042
                     /locus_tag="ACHK1J_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            4029..4218
                     /gene="tnpB"
                     /locus_tag="ACHK1J_004761"
                     /pseudo
     CDS             4029..4218
                     /gene="tnpB"
                     /locus_tag="ACHK1J_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(4221..4409)
                     /locus_tag="ACHK1J_004762"
     CDS             complement(4221..4409)
                     /locus_tag="ACHK1J_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO7913921.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            4944..5085
                     /locus_tag="ACHK1J_004763"
                     /pseudo
     CDS             4944..5085
                     /locus_tag="ACHK1J_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         1429 a         1166 c         1041 g         1592 t
ORIGIN      
        1 actgaatcgt ctctgataaa tactgttgag ctatatctgt actctgtttt tctaaatcag
       61 aagctttctt tgtttcaaga acagatttga ttaactcctt acgttgtgta caccaaatct
      121 cgtcagatga acagtatttt ttattttcgt gtttgttcat tatttattgc tctaactgat
      181 ttatttccct atatccaggg agtagatata tctactccct gcttattatc atttcacgtt
      241 agttaaagat ttttttatat tctcaaattt ggcttggttg atatcactga tcggtttttg
      301 acttgacgtg atgaagtcaa tcataacagc cttcatattc ttaactacat caggatggga
      361 tgcagagaga tctttagttt gttgcagatc gttgatatcg tacagagcta gtttattctc
      421 tgcataggtg taaaccagag aataatcgtt gcttcttaca gtgtatgcaa actggcttaa
      481 gtcttctgta tgtgggttgt gcggataatc atcagactct tttctcacga atttatgata
      541 tccgttccag aatgcagtgt tctcttcgtc ccaccagtga gaataagagg taatccagga
      601 cagatatttg tgaggttcgc cagctgcaga tttcttatca gctcctttct gtacatatgg
      661 caataatgat acaccatcca gtttcagaga agcagggatc ttgatatctg cggcatctaa
      721 agcggtcgga tagaagtcca ttgatgaaac cagtttgtca taatcacccg ttttcagctt
      781 gccattccac cacatgaaca taggtgtatg cataccaccc ggatacggtt ggcctttgta
      841 accagcctgc gcaccattca gtggcagtgg accatcaatt acagcaccgt tatcagaggt
      901 gaacagaatg atggtgttgt cgtactgacc tttttcctta agtgtattca ggatcttttt
      961 aaccccctgg tcaacggagt aaattgacgc ataatagtta tcagcagtct tgctaccagt
     1021 gttgaatttc tcctgatagt ttttaggcgc aggctgatcg ttaggcaggt gtggtgcact
     1081 gtatgccaga taaatcataa atggctgttc cagtttgctt gcacggtcaa ttacaccaat
     1141 tgcttcatca gttaattgat cactaatgta cccttcagct ttaatgtttt cacggttacg
     1201 gaacaatgac ggggagttat aaagtgaaac gccggatgcg tgatagccca tgaagtagtt
     1261 gaagccacga ttctgaggtt gccattcttc tgcggagaag gtggtgaagt tatcgtggta
     1321 gtcacgggtt tgtttttctt caggcacacc tacgttgctg atttttgata agtgccactt
     1381 accgattgct gcagtgtaat aaccatgatt ctgaagtaat tcagacagga atgtttcttc
     1441 cagagggata ccatcctgtg catcagaatt tgaatataca ccgaagcgag caggagctcg
     1501 cgccgtcagg attgcggcac gtgaagggcc agagacaccg tgtgcaacat agccgttagt
     1561 gaattttaca ccttcgtcca tcagggccgc taatgttgga gtggattttt ttgcagcttc
     1621 aattgcttta tcaataccta ttttgtatgt atcaacaaca tttcggttat ccatcgtttt
     1681 agggtcaaat gaactatcat caaaaggcaa ttgtccatac cctaaatcat ccattgttaa
     1741 aacaatgata tttggcttac ctttagtgct gtattccgtc ggcgtgaagt ccgcaaaggc
     1801 aacattggtt tttgttgctt tgagcttaac atcatctgct gcatttgcag tactcatcat
     1861 cagaccagat gctaatataa gtgatattga tgagttaagc agttttttat ttaactgatg
     1921 ttccatatgg tattccttct gagttgcatc acgaacattt attaataata aaagcctctg
     1981 atataaataa aaactatgta aatcgtctaa atggatacct gttaaaactg atacatcata
     2041 ccaacagcaa gtatattatc ggttgcaact ccagcctttt tggtaaattc gctttcatca
     2101 ataaggttaa ttttgtaatc aataagaagt gacatgtttt tgttcagaaa gtagtttgcg
     2161 ccaacatcaa tatactcaac aagagactga tcgccatagt ttactattcc acctttaaca
     2221 tccatccctt tggactgaac gtaagccagt gaaggacgca ggccgaaatc aaacatatac
     2281 tgagccacaa cttcaatatt ccgtgcttta tctgcgatgc ctgcgctacc aaatggagtc
     2341 atatttcggg tttcagagta gtcagctgcc agatacaggt cattatcatt atacttgacc
     2401 ccaacacctg caaactccgc atgttttccc ccggcaatcc agcgaggcgt aataacttct
     2461 tttccttctg aatctttcga tgtcatgaaa cgtttacggc cttctttttc ctgcagactg
     2521 gttctgtcgg cattagtgta agcggcaacg attccaaaac cttcatagtc atagcttacg
     2581 gagaaaccat atccatcacc attagcttca agtaaattat cacggtcatt tttaccctga
     2641 tattgagcag aaaaagtaag tccatcaaca agcccgaaga aatctgtatt gcgccaggtc
     2701 agaactccgg ttgttctgcc ggtcatatag ttatcagagt aagtccagct gtcaccacca
     2761 aagtccggaa gtacgtcagt cagagccccc acatcatagg caacaccata gtttcgccca
     2821 taatcaagtg aaccgagtat attgtggcga atacctgcaa atgccagacg atttttatcg
     2881 ttgcgtactt cactttctgt cttaaacccc aggaactggt attcccactg accaaagcca
     2941 gtcaggctct cattaatttg tgtttcgcct ttgaaaccaa gacgtgcaaa tgttctgtcg
     3001 ccatccatag aaccatcaga gatgtaatga cgggctttaa cgcgaccata aaaatccagt
     3061 ttattaccgt ctttattata gatttctgct gcctgtgttg cctcacacag cattaatgca
     3121 gaaacgacag agcacacaac catacttaat tttcgcataa aaaaatcccc tgtgcacatt
     3181 tttagtatgg ttttaaaaat taaactatgg cgaaaaaatt atcactatgc aaagatgtaa
     3241 caaaatattg ctcagtgatc tatgttgttg tttttatttg aggttgtata ttttctagtg
     3301 tgtttgactt ttctttctca agtgttattt cttgaccttt atcacagaac catctccctg
     3361 gatatcccca tcacattgca tgaaaagcaa gttttaccaa tcttgatttt cagtgttaaa
     3421 taagattaat aactgtaatt ctgcaaaaaa aagaacacca tatgtagtat gactacgtat
     3481 tccactctgt tcgttcacgg actggcatcg aagtctgagg tgtactggca atagcggata
     3541 cagtcatttg ttcttttttt aagcattcat ccgatgatac ttttcctgaa tgctaccgga
     3601 gtggtattcc cctgccgtga gggagggcgc tgacggttat aaaaatcctg atatataccc
     3661 attcccgtat gacagcgatg gcgtcacatc tctgttctac catcttcaca gaaatatttc
     3721 gttcattagc attccggtat tcgtataacc accttcggtg gagaaagaat aaacaatccc
     3781 tggaatgcga tgagcgcttg accagaaaag catcatattg cttcatggcg catcagcgac
     3841 cacaggatgc cgcaaaagcc gaaaaacgga ccagagcgcc tgcaatcatt cctcgcctca
     3901 gtatctgttg cccatggagt ggccgataac agatcattgt cggatgagcc ttatcccctt
     3961 ccttcccggt ggtatccgga tgtgctgcca gccttctcag ttaaccgatg ttttccgggc
     4021 tttttgccat actgaggact gataacgtat tcctggtcgt aaaacccgtc aatatgcgcc
     4081 ggggcatcga caaaactgac acagtacgtc cggaaagaac ttaacgctgc atggcacgac
     4141 aatgctgcct ttgtctttac caacaaggct cgttcccgca taaaagttct acgccgggat
     4201 aaacatggcg tatggctgcg tcaggccttc actacttcac accggaagtc ttccaccctg
     4261 acacgaatat accgtccggg ctctttcgca ggtgcaaaca gtttttcaca ttcggcgaat
     4321 gtcatttctg aatcctggta acgcaaccac tgaaccggca tattaccggg tttgattatt
     4381 gtcgcagtta ttttatatcg tttccccata tcgcctctca tcctgacaac aaaattaccg
     4441 gctgagccat cactccggta aagcatgcga aaaaaagtga gcgtctcagc aatggctcat
     4501 gcgatgattc tgtacgggca ccgatggcac ctcagaatac aacaaagata tcagagtaac
     4561 atgtctctct cgttattttt cagagttctt gacaccctcc ttctgccaca ttgtcaacgc
     4621 cagtggagac ttctgctgtc agttttttca tccatacaga gtgacattaa ttatcctgaa
     4681 caaaacgaaa tatcctgaat atatttattt tctcctggtg ccggagacat gatgatgtac
     4741 aggctcaggt gcggtgtgaa tgtaaaatgg tatgttaatt attcatttct gaatgcaaac
     4801 tgaatgcaaa cttattccga attgaattct cctgacacca catgataaaa atgaggatat
     4861 aataaaatac ttctcttctg gatgtgccat tacctgccgt tgctgtaata cggcgggtat
     4921 tttttactga catatcggga agccagcaga cgttcgtagc tacatccccg aaagtggcac
     4981 actgcaaact ataatacgag taaaacatcg atcaccgtag ctgatgtgtt caacgatcgt
     5041 gcactagcgt tctgcgattc tcagggactg cctgttctga gaatcggcga gtccgcgatc
     5101 ggatacgatg cgatttcgca gtgagtcagg gattgcatct gaaaaccctc cggctatgtc
     5161 ggtaaatatt tatttctatg aaccgtacga ttcagatagc attcgcccgg ccccgtaaga
     5221 ataatctc
//
LOCUS       DAZGBC010000251         5219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGBC010000251 DAZGBC010000000
VERSION     DAZGBC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.253"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..1428
                     /gene="garP"
                     /locus_tag="ACHK1J_004764"
     CDS             94..1428
                     /gene="garP"
                     /locus_tag="ACHK1J_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO7913922.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSGEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1444..2214
                     /gene="garL"
                     /locus_tag="ACHK1J_004765"
     CDS             1444..2214
                     /gene="garL"
                     /locus_tag="ACHK1J_004765"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO7913923.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2244..3134
                     /gene="garR"
                     /locus_tag="ACHK1J_004766"
     CDS             2244..3134
                     /gene="garR"
                     /locus_tag="ACHK1J_004766"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7913924.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVVLGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3231..4376
                     /gene="garK"
                     /locus_tag="ACHK1J_004767"
     CDS             3231..4376
                     /gene="garK"
                     /locus_tag="ACHK1J_004767"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO7913925.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4409..4785
                     /gene="rnpB"
                     /locus_tag="ACHK1J_004768"
     ncRNA           4409..4785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK1J_004768"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1232 a         1242 c         1484 g         1261 t
ORIGIN      
        1 ccgagttcat tgtcgttacg gcataaatga aactcataaa taacaccttc cccataataa
       61 taaaaatcag cataagtacc cgaggtaaat aaaatgattc tggacaccgt tgacgaaaaa
      121 aagaaaggcg tgcatacccg ctatttaata ttactgatta tttttattgt taccgccgtt
      181 aactacgccg atcgtgcaac gctgtctatt gctggtaccg aagtggcaaa agagttgcag
      241 ttaagtgcgg tttcgatggg ttacatcttc tctgcttttg gctgggccta cttgctgatg
      301 caaatccccg gcggctggct gcttgataag tttggctcga aaaaagttta cacctacagc
      361 ctctttttct ggtcgctatt caccttcctg caaggctttg ttgatatgtt cccgctggcc
      421 tgggcaggga tctccatgtt ctttatgcgc tttatgctcg gcttctcgga agcgccatca
      481 ttcccggcga acgcccgtat tgtcgccgcc tggttcccga cgaaagaacg tggtactgcc
      541 tccgccatct ttaactcggc gcaatatttc tcgctggcgc tcttttcgcc tctgcttggc
      601 tggctgactt tcgcctgggg ctgggagcac gtctttaccg ttatgggggt gattggtttt
      661 gtgctgacgg cgctgtggat caaattgatt cataacccga cagatcaccc acgtatgtct
      721 ggggaagaac tgaagtttat ctctgaaaat ggcgcggtgg tcgatatgga ccacaaaaag
      781 ccgggcagcg cggcagcaag cggtcccaaa ctgcattaca tcaagcaatt gctctctaac
      841 cgcatgatgt tgggcgtatt tttcggacaa tattttatca acaccatcac ctggttcttc
      901 ctcacctggt tcccgattta tctggtgcag gaaaaaggca tgtcgattct gaaagtgggt
      961 ctggtcgcct cgattccagc actgtgtggt tttgcgggcg gcgtgctggg aggtgtcttc
     1021 tcggattatc tgatcaaacg cggtttatcc ctgaccctgg cacgtaagct accgattgtg
     1081 ctgggaatgt tgctggcttc caccatcatc ttatgtaact acaccaacaa caccacgctg
     1141 gtggtcatgc tgatggcgct ggctttcttt ggcaaaggat ttggtgcgct gggctggccg
     1201 gtgatttctg acaccgcgcc gaaagagatt gttggcctct gcggcggcgt ctttaacgtc
     1261 tttggcaatg ttgcctccat tgtcactcca ctggtgattg gctacctggt aagtgaactg
     1321 cactccttca atgcagcact ggttttcgtg ggatgttcag cgctgatggc gatggtctgc
     1381 tacctcttcg tagttggcga cattaaacgt atggaattgc agaaataagc aaaggtataa
     1441 gcgatgaata acgatgtttt cccgaataaa ttcaaagccg cattggctgc gaaacaggta
     1501 caaattggtt gctggtcagc actctctaac ccgattagca ctgaagttct tggtttggct
     1561 gggtttgact ggctggtgct ggatggcgaa catgcgccaa acgatatctc cacgtttatt
     1621 ccgcagttaa tggctctgaa aggcagcgcc agcgcgccag tagtacgagt gccgaccaac
     1681 gagccggtaa ttattaagcg tcttctggat atcggtttct ataacttcct gattcctttt
     1741 gtagaaacaa aagaggaagc agagcaggcg gtggcatcga cgcgttatcc gccagaaggc
     1801 attcgcggcg tctccgtttc tcaccgcgcc aatatgtttg gcaccgtggc ggattatttc
     1861 gctcagtcga acaagaacat cactattctg gtccagatag aaagtcagca gggcgtagat
     1921 aacgtcgatg ctattgccgc taccgaaggc gtagacggca tcttcgtcgg ccccagcgat
     1981 ctggccgcgg cattaggcca tctcggcaat gcatcacacc cggatgtaca aaaagcaatt
     2041 cagcacattt ttaaccgtgc cagcgcgcac ggcaaaccca gcggtatcct cgcgccggtc
     2101 gaagccgatg cgcgtcgtta tctggaatgg ggcgcgacgt ttgtggctgt cggcagcgat
     2161 ctcggcgtct tccgctctgc cactcagaaa ctggctgata cctttaaaaa ataaccacca
     2221 ccgcaacaag agagatgatt gatatgacta tgaaagttgg ttttattggc ctggggatta
     2281 tgggtaaacc aatgagtaaa aaccttctga aagcaggtta ctcgctggtg gttgctgacc
     2341 gtaacccaga agctattgct gacgtgattg ctgcaggtgc agaaacagct tctacggcta
     2401 aagcgatcgc tgaacagtgc gacgtcatca taaccatgct gccaaactcc cctcatgtga
     2461 aagaggtggt gctgggtgag aatggcatta ttgaaggcgc gaagccaggt acggtattga
     2521 tcgatatgag ttctatcgca ccgctggcaa gccgtgaaat cagcgaagcg ctgaaagcga
     2581 aaggcattga tatgctggat gctccggtga gcggcggtga accgaaagcc atcgacggta
     2641 cgctgtcagt gatggtgggc ggcgacaagg ctattttcga caaatactat gatttgatga
     2701 aagcgatggc gggttccgtg gtgcataccg gggaaatcgg tgcaggtaac gtcactaaac
     2761 tggcaaatca ggtcattgtg gcgctgaata ttgccgcgat gtcagaagcg ttaacgctgg
     2821 caactaaagc gggcgttaac ccggacctgg tttatcaggc aattcgtggt gggctggcgg
     2881 gcagtaccgt gctggatgcc aaagcgccga tggtgatgga ccgcaacttc aagccgggct
     2941 tccgtattga tctgcatatt aaggatctgg cgaatgcgct ggatacttct cacggtgtcg
     3001 gcgcgcaact gccgctcaca gctgcggtta tggagatgat gcaggcactg cgtgcagatg
     3061 gtttaggaac ggcggatcat agcgccctgg cgtgctacta cgaaaaactg gcgaaagtcg
     3121 aagttactcg ttaatgacgt cgtgcccgga tggcgtattg caatccgggc ctggaatcca
     3181 ggccgaataa gagaaacgcc ctccggcgtc atgtggtaac aggcataggt atgaaaatcg
     3241 taatcgcccc agactcttat aaagaaagtt tatctgccag cgaggttgcg caggcgatag
     3301 aaaaaggatt tcgggaaatt tttcctgatg cacagtacgt ttctgttcct gttgccgacg
     3361 gtggcgaagg aacggtggaa gcgatgattg cagccaccca gggttccgaa cgtcacgcct
     3421 gggttacagg gccgctgggc gagaaagtga atgccagttg ggggatctcc ggcgatggca
     3481 aaaccgcgtt tattgaaatg gcggcggcca gtgggctgga gctggtacct gcggaaaaac
     3541 gcgatccact cgtgaccact tcacgcggca caggcgagtt aatcctgcag gcgctggaga
     3601 gcggcgcgac aaacattatt atcggcattg gcggcagcgc tacaaatgat ggcggcgcag
     3661 gcatggtaca ggcgctgggg gcgaaattat gcgacgccaa cggcaatgaa attggttttg
     3721 gcggcggtag tcttaatact ctgaatgata ttgatatttc cggcctcgat ccgcgcttaa
     3781 aagattgcgt cattcgcgtc gcttgtgatg tcaccaatcc gctggtgggc gataacggcg
     3841 catcgcgcat ctttggccca caaaagggag ccagtgaagc gatgattgtt gagctggaca
     3901 ataacctctc tcactatgcc gaggtcatta aaaaagcgct gcatgttgat gtgaaagatg
     3961 tccccggcgc aggagctgcg ggtggtatgg gcgcggcgct aatggcgttt cttggtgcgg
     4021 aactgaaaag tggtattgaa atcgtcacta cggcgctgaa tctggaggaa catattcacg
     4081 attgtacgct ggtgatcacc ggtgaagggc gtattgacag ccagagtatt cacggcaagg
     4141 taccgattgg tgtcgcaaac gtggcgaaga agtaccataa accggtgatt ggcattgcgg
     4201 gtagcctgac cgatgatgtt ggcgttgtac atcagcatgg cattgatgcg gtcttcagcg
     4261 tattgaccag cataggtacg ttggacgaag cattccgcgg ggcttatgac aatatctgcc
     4321 gtgcttcacg taatatcgcc gcgacactgg cgattggaat gcgcaacgcg gggtgacaag
     4381 ggcgcgcaaa ccctctatac tgcgcgccga agctgaccag acagtcgccg cttcgtcgtc
     4441 gtcctcttcg ggggagacgg gcggagggga ggaaagtccg ggctccatag ggcagggtgc
     4501 caggtaacgc ctggggggga aacccacgac cagtgcaaca gagagcaaac cgccgatggc
     4561 ccgcgcaagc gggatcaggt aagggtgaaa gggtgcggta agagcgcacc gcgcggctgg
     4621 taacagtccg tggcacggta aactccaccc ggagcaaggc caaatagggg ttcataaggt
     4681 acggcccgta ctgaacccgg gtaggctgct tgagccagtg agcgattgct ggcctagatg
     4741 aatgactgtc cacgacagaa cccggcttat cggtcagttt cacctgattt acgtaaaaac
     4801 ccgccagcgt aaactggcgg gtttttgctt ttggaggggc ggaaagatga atgactgtcc
     4861 acgacgctag acccaaaaga aagcggctta tcggtcagtt tcacctgatg tacgtaataa
     4921 accgttccgg cgggtttcag actgttgagt gcgctttatt catgccggat gcggcgtgaa
     4981 cgccttatcc tgcctacaaa agcacgcaaa ttcaatatat tgcagagatg gcgtaggcct
     5041 gataagcgta gcgcatcagg catttttgcg gttgccatca gtttcaaacc cgcgtcgata
     5101 agtttttgct tttggtgcac agggtaagat gaatatctac ggtattaata atagactttg
     5161 taagaagtta ttaagtctat attgcattta aaaggtcgta atcatacgta tagtcctct
//
LOCUS       DAZGBC010000252         5044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGBC010000252 DAZGBC010000000
VERSION     DAZGBC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.254"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(170..325)
                     /locus_tag="ACHK1J_004769"
     CDS             complement(170..325)
                     /locus_tag="ACHK1J_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7913926.1"
                     /translation="MKQQKAMLIALIVICLTVILTVLVARKDLCDVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(525..950)
                     /locus_tag="ACHK1J_004770"
     CDS             complement(525..950)
                     /locus_tag="ACHK1J_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7913927.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            complement(1029..1892)
                     /locus_tag="ACHK1J_004771"
     CDS             complement(1029..1892)
                     /locus_tag="ACHK1J_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7913928.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGID
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1892..2860)
                     /locus_tag="ACHK1J_004772"
     CDS             complement(1892..2860)
                     /locus_tag="ACHK1J_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO7913929.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRIQRRFSALTSLQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2862..4520)
                     /locus_tag="ACHK1J_004773"
     CDS             complement(2862..4520)
                     /locus_tag="ACHK1J_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO7913930.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            complement(4554..4922)
                     /locus_tag="ACHK1J_004774"
     CDS             complement(4554..4922)
                     /locus_tag="ACHK1J_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913931.1"
                     /translation="MRAELKQVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT         1194 a         1396 c         1103 g         1351 t
ORIGIN      
        1 cgtcctttta tgaaaatcaa cgttttttga gcactcgcac acaaaaaatt accgcacggg
       61 ttgcaacgac aacctgcatt ttgcggagca tatgacaact aaagtacggg tgcgttgagg
      121 atgcctgaca catcagaggg ggcgggagat tactcccccg ccaggtctct tacttctcag
      181 attcgtagtc tacgaagaca gcgacctccg tctggccggt tcggattcgt acatcgcaga
      241 ggtctttcct cgctaccagt accgtcagaa tgacggttaa acagatgacg atcagggcga
      301 ttaacatcgc cttttgctgc ttcatagcct gcttctcctt gcctttcggc acgtaagagg
      361 ctaacctaca tgtgttcagc atgaaattgg cctcagatta atgttaagcg tcttgcagga
      421 cgcgtaatgt taactggggc ttttctccgt ctgccttacg gcagcatgcc cgaggcagac
      481 accctcaagc acccgcagtc attctacagt aaaaccccgc gctttcaatt tcccgtatgt
      541 ccggaaaact gactgacctg tgcccctgat cgttcacgct caacgtatct gtccatttcc
      601 agttttattc cctgcataat caacatcccc tcaataacac cttcagcttt ttgcagtacc
      661 cgcccggccc agcaatcaga gcgctcatgc ttacggccca gtcccataag cgtcatcccg
      721 aacacataat aatcatacag aaaatcatgc aaatcgctgt tgttgacgtt cagttgcgcc
      781 atgcaattgc tgataatcag cgcatcatca tcggtacact gaggccgtga tttaaccttt
      841 gatgggatca ctcccttaaa tccggcagcg acgccggacc atgccacact ctcatgatta
      901 tttaccaccc atgcacccca tcgggacaga acctgttgga tatcacgcat tattctgtac
      961 tccgctgtaa ccatactttt tctgatttga atgctcaggg ggaaaatgtc tactggatat
     1021 ctccggcttc attgctcaca ctccccaacc agattgagaa tgaccgctga accattgtct
     1081 tccatgtact catcttttcc gcttgccaga aaccagcgac acacttcaac cgcttcatca
     1141 cgcgtcacag gctttatttt tcccagcaaa ttttcgagcc agaactgccg atcatatact
     1201 gactcatgat gttcacctgt gaaaccgtat ttgtcccctg tttctctccc cgctgtctgg
     1261 cgcatctcat aaagccagtc ccagtaaata aactcacgaa caacgtctga caatgtatga
     1321 ggctctggca gtacatcacg atagccatca acatatgcac gacgctgatc atcaatttca
     1381 tttgagcgaa caccgtcaat acttccggct tttttctctg ccgcagtcca gccccagtag
     1441 taatcgtcga taaatttcgg ggaggtgcga attatccgct cagcctccac atcatcgagt
     1501 gctgcctcat agctgccaaa tgtggccctg actgatgcgg cttttttgat attctcacgg
     1561 gcattcctga tcgcctgcgc cgggttatct atgcctatgg ttgcgaaagc aatctggaaa
     1621 ggatcaacat taatctcctg caaataacgt ccataccgtt cactcgcttc ttccggcgtt
     1681 attttgagtt tctccagtgc tgcttcggct gcgtccagat gtacggcctc gttcaggtga
     1741 ataacctcca gaatccagag ataagcatct gtctgtttat gtccggtgat cctcctttgt
     1801 tctggcagag gtttgatatt tgccggaacg gcgctgtacg ctgccgtcgg gatagtgaat
     1861 aaagctttat gtacctgatt gtccgtacgc atcacgcaac cgcctttttt ctgtaaaaaa
     1921 ccagttcccg aacctgatcg ccgttcatga gcatattgtt gaaatcatca tgatcaggcc
     1981 accggacact tacccgctga atgtcatttc gggcctggag atttgcatgt gcgcattcac
     2041 aggccgcagc cattccggta gcactgtatt catcgcgatc ggcaaaaata atcagatggg
     2101 tgactcccgc cggtacgcgg aactttttca taaaaccact gttaatggtt gcccaggtat
     2161 ttactttgta aatctgatgt gcggacaacg cggtttcgat accttcagct atacccagtg
     2221 tactggctac cggaaacatc ctgatagcaa ctgaccgggc atggtccaga tagttatcgt
     2281 cctgtaaaga tttgagcctt ctggcgctga taccaatgtc tgctttttta gctccatcaa
     2341 gcaatgtctg atgcaggtaa cacagttctc ccttgtcgtc ggtcgccagg gcataaagag
     2401 actggaacat gcgcccgtta taattttgct ggctgttgaa gcgaactccc tcaaccggaa
     2461 gactgaatat gccacgggaa cgaagatatt cagcaccaga tgttccctgt agcgacgtga
     2521 gcgctgaaaa tcgacgctga atgcgcgatc tcaggctttc cgacgtacag atcacaggcg
     2581 gtttatctct ccggtattca ttccccgtga tgcggtctat ttcagcgcaa atttcatgaa
     2641 acgctctttt ttgtgtcaga gcgatcagtt tcatgccgtc accgctacca caaacacaga
     2701 tccacgttcc ggtgccatca cggtcatcaa tacggaactt acctctggca ccacaaaccg
     2761 gacattcccc tttgaagtgc tttttacccg tgactggcgg cagtccgtaa tgttttaaaa
     2821 tttcaggcca gtggcctttc gctgcttctg ctgttttcat cttactgact cagactgttt
     2881 ctgtttttct gaagttgttg ctttgcctgc atgatctgcc acgactcact gccttccggc
     2941 accccagacc ggacaccatg aagcagggac agagaaagcg cttcggggga tttttccgtc
     3001 atcttctctg cccggctacg ccctctggca aaccggatga atttgtgtct gatgtaatta
     3061 ctgacctccg gggtgatttc catcggaaaa ctgctcagcc cgtcaggcca ttcgccgaac
     3121 ttctcccgga aagtgtgagc gcaccatcca tcgctgaccg gtcgccccag agaggcacgc
     3181 tgacgctgat agaatttgat ctgactccac caggcctgtt tgtcgctttt ggtgtaaatt
     3241 ttttcgtcct tgctgagttt cctgatattg cggcgggtat ctgtgttcac atcctcacca
     3301 gccagcggtt taaatccgca tttcggacag acataaacac ctgccggttt catgaaatga
     3361 caccccggac attctttcgg cagtttttct tcccgaattt cgttaccacg tgatgatgac
     3421 acctgcatcc cgtcgttttt gccgggcagt tcgtcatact caatattttc aggaaatccc
     3481 aggcgatgaa ccgtaccgga atggtcgaaa atcaggcatg actccttgcc tggcgctgta
     3541 cgcaatcctc tcccaatcgc ctgaagccag cgaatctctg attttgtcgg gcgtgcgtaa
     3601 atgatgcacc ggacatcact gtcaaatccc gccaccagaa cgccaacgct gacaatgatt
     3661 ttcgtcgctc ccgtttcaaa acgactgatc attaactgac gctcatcatg tggcgtttca
     3721 gcaaccatca cctctgcgtt aattccggct ttgttgaact gcatggtcac gaagctggca
     3781 tgcgttttat tgacgcagaa cgccactgtc ggtaaatccc ttccgttaca cagccagtta
     3841 tcgacaatat cgccaaccag atccgcgccg cacataatct ctgccagttg tgattcgtcg
     3901 taatccctgc cgtactccat tgagactttc gtttttacac cttccagatc cggtttcgtg
     3961 ggcgcataaa attcgtaatt gctcagttcg ccacgctgga tcagttcccc tatagtggtc
     4021 ggcttgagaa gacgctggta ataacgcccc aggaacgatg aaaagggtgt accggacaac
     4081 ccaatcacct tcacatttcc ctctgcaacc agtcgttcga tttccgtgag gatgcggcgc
     4141 ttacggagat gagcttcatc aataatcagc aggtcgatat tctgaggaaa atcacggcga
     4201 atcagcgtat ccgcactggc aatctgaatc agtcgtgacg ggtcgttatt gggatgatca
     4261 cgccagataa agctgatttc atccccggaa agcccgtact gaacaaagcg ctgtgctgtc
     4321 tggttgatca aaatggtata aggaacgaca aacagaacgc gcatgccacg gcttaccaga
     4381 ccagcagtga tgaacgccgc caggccggtt ttaccactcc ctgtaggcat gtacatcatg
     4441 aaggactgat atgttttcca gtcatgacgc aacatattca gcgcacgttc ctgagcaaaa
     4501 ttcggcttga tattcagcat ggtattctcc cgatgacagg gctgatacag tgattactta
     4561 aaccgggtcg cccggatgac atccacacca tcggcgatat aacgctgtgg atgtttgcca
     4621 tcacggagaa aaacaaatcc cgaaatgcct tcaggaagcg ctcgaatttt catcagtgaa
     4681 cgctgacggg gcgatcggtt atccacctca actgcgcaac gctgaccatc gggagaaata
     4741 accagaagat cgataaatcc cctgcgatca cctttcagcc tgacagtgaa acagcgttgc
     4801 aactcacagc ccagagattt gattttcttt tgcagaacgt agtcgaatgt gaccttgtcc
     4861 attccatgtc cgaattcctc ttccagaagg ttgattagta cctgtttcag ttcagctctc
     4921 atattttaac catgatgtac tagtttgctg atacgtccgt tttttcaggg atgccccttt
     4981 agatcgagat ctacctaacc tctgtacccg tctgttggaa gatcccgttc cagtgcttcg
     5041 cact
//
LOCUS       DAZGBC010000253         5040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGBC010000253 DAZGBC010000000
VERSION     DAZGBC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.255"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(26..250)
                     /gene="cspD"
                     /locus_tag="ACHK1J_004775"
     CDS             complement(26..250)
                     /gene="cspD"
                     /locus_tag="ACHK1J_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO7913932.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            400..474
                     /gene="yljB"
                     /locus_tag="ACHK1J_004776"
     CDS             400..474
                     /gene="yljB"
                     /locus_tag="ACHK1J_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO7913933.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            573..893
                     /gene="clpS"
                     /locus_tag="ACHK1J_004777"
     CDS             573..893
                     /gene="clpS"
                     /locus_tag="ACHK1J_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO7913934.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            924..3200
                     /gene="clpA"
                     /locus_tag="ACHK1J_004778"
     CDS             924..3200
                     /gene="clpA"
                     /locus_tag="ACHK1J_004778"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO7913935.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            3779..4987
                     /locus_tag="ACHK1J_004779"
     CDS             3779..4987
                     /locus_tag="ACHK1J_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7913936.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT         1342 a         1097 c         1386 g         1215 t
ORIGIN      
        1 ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg acgggcacaa
       61 taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg gattgtccag
      121 cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa atatcttcgc
      181 cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac ttaacagtac
      241 ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga ataaaccggg
      301 gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta gagaattagg
      361 gcgagccgtc aagcatttga caggggacaa ggggcaggta tgaatcaaaa atttgaagca
      421 gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag atgatagtta
      481 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
      541 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
      601 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
      661 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
      721 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
      781 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
      841 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
      901 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
      961 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     1021 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     1081 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     1141 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     1201 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     1261 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     1321 atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     1381 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     1441 cgacgaacct gaatcagctt gcgcgtgtgg gcggaatcga cccactgatt ggtcgtgaga
     1501 aggagcttga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     1561 tgggggaatc tggtgttggt aaaactgcga ttgcggaagg tcttgcctgg cgaattgttc
     1621 agggtgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     1681 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     1741 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     1801 cgggtgcagc gtctggtggt caggtcgatg cggctaacct aatcaaaccg ttgctctcca
     1861 gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     1921 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcgatcgaag
     1981 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     2041 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt aacgatcgtc
     2101 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     2161 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     2221 ttgcacgcat tccagagaag agtgtttctc agagtgatcg tgataccctg aaaaacctcg
     2281 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     2341 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     2401 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     2461 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     2521 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     2581 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccagacg
     2641 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     2701 aagcggactt ccgtaacgtg gtgctggtga tgaccactaa cgccggggta cgggaaactg
     2761 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     2821 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg ttcgatcatc
     2881 tgtcaactga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     2941 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     3001 aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag gacaacctga
     3061 aaaaaccgct tgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     3121 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     3181 aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtgaagtccg taatctcgtc
     3241 aggggttacg gactttttat ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     3301 aggtaagttg ttgataatta gtgctgctgg aaggtaagga taaaaaaggg tgctgcagga
     3361 gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg agttcactgc
     3421 cgtacaggca gcttagaaaa acctaccgtc ttggctagcg gttgcagcga acgttcactg
     3481 ccgtacaggc agcttagaaa ttcactggta acatactcca cccgcccacc atgttcactg
     3541 ccgtacaggc agataaaatg cgaaaaaaaa agctcgcact ttcgtacgag ctcttcttta
     3601 aatatggcgg tgagggggac cgcctgaaat aaatctgact taatccggga gtgttatata
     3661 acttcattat aaacaatgca ttacaatttt tgctgtccga tgttatctaa tgtattctta
     3721 gtgaagtgag agactaatgg catactggat ggcataccag agtaacagga attagcgtat
     3781 ggcagtattg acggatacta aagcaaggca tatcaaacct gatgacaaac cattgcccca
     3841 tgggggaatt acaggactga ctcttcatcc ttcttcagta aaggggcggg ggaaatgggt
     3901 ttttcgttat gtaagtccgg tgacacaaaa aaggcgtaat gctggattgg gtacttaccc
     3961 tgaggtcagt attgctgaag ctgcacgtac tgcccggata atgcgagagc aacttgctgc
     4021 aggtgatgat cctctggaga ttaaaaaggc tgaagctgaa aaagttgtta tcccaacatt
     4081 tgccgatgca gcccggcgtg tacatgcaga attgtcaccg ggatgggaaa acccaaagca
     4141 tgtcaggcag tggttgtcga cgcttgaaaa ttacgtgttt cctcaactgg gagcaaaaac
     4201 gctggatacg attacggctg cggacgtggc agagacactg cgtccagtct ggttgacctt
     4261 atcagaaacg gcaagccggg ttaaacagcg cattcatgtt gttatgcagt ggggctgggc
     4321 gcatggtttt tgtgtggcga atcctgttga tgtggttgat catttgcttc cacagcaatc
     4381 aagaggacgt gatgaacacc agccggcaat gccctggagg cagttaccgc tttttgtggc
     4441 gaccagtgtg tatacagatg aaccttataa tgttacccgg gcactgttat taatggtgat
     4501 actgacagca acccgctcgg gcgaagcaag gggaatgcgc tgggctgaaa ttgattttca
     4561 taagcggata tggacgatac ccgcagaaag aatgaaagcc aggatacagc atcgtgttcc
     4621 tttatcccga caggccattc acgttctgga aaatatacgt ggtctgcatg acgaactggt
     4681 gtttccttct cccagaaagc agcagatcct ttcagatatg gtgttgacga gttttctacg
     4741 taaaaagaag gccatcagtg atatacccgg acgagtggct acagcacatg gttttcgttc
     4801 aacattcagg gactggtgta gcgaacaggg atattcgcgg gatttggcgg aaagggcgct
     4861 tgcccacacg ctgaaaaata aggttgaggc ggcatatcac cggactgatc tgctggatca
     4921 gcgtataccg atgatgcagg catgggcgga ttatgtgatg tctcagatta tggaaaacca
     4981 gcgatgaaat cacatctcac agatttgcag atgtcgtctg taatcacgaa aaatatactt
//
LOCUS       DAZGBC010000254         5005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGBC010000254 DAZGBC010000000
VERSION     DAZGBC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.256"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(321..1352)
                     /gene="rsmC"
                     /locus_tag="ACHK1J_004780"
     CDS             complement(321..1352)
                     /gene="rsmC"
                     /locus_tag="ACHK1J_004780"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO7913937.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGIEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1455..1868
                     /gene="holD"
                     /locus_tag="ACHK1J_004781"
     CDS             1455..1868
                     /gene="holD"
                     /locus_tag="ACHK1J_004781"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO7913938.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1837..2283
                     /gene="rimI"
                     /locus_tag="ACHK1J_004782"
     CDS             1837..2283
                     /gene="rimI"
                     /locus_tag="ACHK1J_004782"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7913939.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2298..2975
                     /gene="yjjG"
                     /locus_tag="ACHK1J_004783"
     CDS             2298..2975
                     /gene="yjjG"
                     /locus_tag="ACHK1J_004783"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO7913940.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3361..4584
                     /locus_tag="ACHK1J_004784"
     CDS             3361..4584
                     /locus_tag="ACHK1J_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7913941.1"
                     /translation="MALSDVWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKVTEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGEITGEPL
                     KRPIIEPVEELIKYAISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRCLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYLAWW
                     KRVESIVNCTISDSN"
BASE COUNT         1307 a         1196 c         1252 g         1250 t
ORIGIN      
        1 gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt cgcatgccat
       61 caattcttaa aaagaattgc taccacggag gcgcattcta gtggttttca gcttttcgtc
      121 aatagttaat tatcgccgga ggtgtaattg ctggaaaaat gtccatcagg aaactagcgt
      181 gcaggtttgg taggcatgcg ggggcagatg ccagatgcga cgctggcgcg tcttatctgg
      241 cctacgaagg gctaacgtgc aggttttgta ggtcggataa ggcgttcacg ccgcatccga
      301 cacagtattc ggcgggatac ttaacctttc ttcgcctggc gggtcataat ggcgcgatac
      361 accttgaagc gccctgtttg cgcgataact tcatggaagc caaatgtctc atccagcacg
      421 tccggataag gcaggaaggc gttcgctaca attcgcagct cgccgccgct attaagatga
      481 cgcaccgcgc cgcgaatcag cgtttgcgcc gcatccaggc tggtttgcat cccatcgtgg
      541 aacggcggat tggagatgat catatcaaaa cgacctttta cctcggaaaa gacgttgctg
      601 gcaaagactt caccttcaat accgttggcg gcaagcgtcg cgcggctggc ttcaactgct
      661 ggcgcagaga catcgcacaa ggtgagacga attttcggcg aatggcgcgc aaaggcaact
      721 gaaagcacac ccgcgccaca gccgacatcc agcactttac ctttcgtgtg cggagttaac
      781 gtcgagagca gcaactggct accgacatcc agaccgtcgc ggctaaacac gccaggcagc
      841 gttttgaccg tcaggccatc gacgctgtat tcgccccaga atttatccgc atcaaatact
      901 ggctgttttt ccagacgacc aaaatagagt ccacagcgac gagcgctgtc gactttattc
      961 aacggcgcat aatctgccag catctgctcg gcgctgcgca cgccgctgcg gttctcgcca
     1021 acgacaaaaa tatctgtccc cactggcagc agagaaagta aattcatcaa ctggaactgg
     1081 gcttccggtt tgttcttcgg ccagtagtaa atcagcgtat cgcaatctgc gacgtcatcc
     1141 gccgtggcga ccagactgaa acgggcgtta tcccccatct ggcggcttaa tacctgccag
     1201 tggtggaatt gctgggtatg agcacggctg gccgcggtat ctaaacgcgc gggcaggtca
     1261 tcctgtaagt ctccggcaaa cagaatacgg ctttgttcga aatcatcact gtgacgcagc
     1321 aagacttcac ttgccggggt aaatgcagac atggaatgct cctcaattga tactggcggc
     1381 gattatagcc atatgttggc gcggtatcga cgaatttgct atatttgcgc ccctgacaac
     1441 aggagcgatt cgctatgaca tcccgacgag actggcagtt acagcaactg ggcattaccc
     1501 agtggtcgct gcgtcgccct ggcgcgttgc agggggagat tgccattgcg atcccggcac
     1561 acgtccgtct ggtgatggtg gcaaacgatc ttcccgccct gactgatcct ttagtgagcg
     1621 atgttctgcg cgcattaacc gtcagccccg atcaagtgct gcaactgacg ccagaaaaaa
     1681 tcgcgatgct gccgcaaggc agtcgctgca acagctggcg gttgggtact gacgaaccgc
     1741 tatcactgga aggcgctcag gtggcatcac cggcgctcac cgaattacgg gcaaacccaa
     1801 cggcacgcgc cgcgttatgg caacaaattt gcacatatga acacgatttc ttccctcgaa
     1861 acgactgatt taccagcggc ttaccacatt gaacaacgcg cccacgcctt tccgtggagt
     1921 gaaaaaacct ttgccagcaa ccagggcgag cgttatctca actttcagtt aacgcaaaac
     1981 ggcaaaatgg cggcgtttgc gattacgcaa gtggtgctgg atgaagctac attgttcaat
     2041 attgcggtcg atcctgacta tcagcgtcag ggattgggaa gggcgctgct ggaacatctg
     2101 atcgacgaac tggaaaaacg cggcgtggcg acgctctggc tggaagtccg tgcctcaaac
     2161 gctgccgcca ttgccctgta cgaaagttta ggctttaacg aggcgacgat tcgccgcaat
     2221 tactacccca ccacggacgg tcgcgaagac gccatcatca tggcgttgcc aatcagtatg
     2281 taagacaagg tggaataatg aagtgggact ggattttctt tgatgccgat gaaacgttgt
     2341 ttacctttga ctcattcacc ggcctgcagc ggatgtttct tgattacagc gtcaccttta
     2401 ccgctgaaga ttttcaggac tatcaggccg ttaacaagcc actgtgggtg gattatcaaa
     2461 acggcgcgat cacttcatta cagcttcagc acgggcggtt tgagagctgg gccgaacggc
     2521 tgaacgtcga gccgggtaaa ctcaacgaag cctttattaa tgcgatggcg gaaatctgta
     2581 cgccgctgcc gggcgcggtt tctctgctta acgccattcg tggcaacgcc aaaatcggca
     2641 tcatcaccaa cggctttagc gccttgcagc aagtgcgtct ggaacgtacg ggcctgcgtg
     2701 attacttcga tttgctggtg atttccgaag aagttggcgt tgccaaaccg aataagaaaa
     2761 ttttcgatta tgcgctggaa caggcgggca atcctgaccg ttcacgcgtg ctgatggttg
     2821 gcgacactgc cgagtccgat attctcggtg gcatcaacgc cgggcttgcg acctgctggc
     2881 tgaatgcgca caatcgcgag caaccagaag gcatcgcgcc cacctggacc gtttcttcgt
     2941 tgcacgaact ggagcagctc ctgtgtaaac actgattgcc tcccccccgt tgatgggtaa
     3001 aatagccgca atttttcgtt ttcaacaagc gcggcgcgat gccgcttact caagaagaaa
     3061 gaattatgac gttgtctcct tatttgcaag aggtggcgaa gcgccgcact tttgccatta
     3121 tttctcaccc caataattaa gcccaaatta aagctctttt actctttcaa aatcctttca
     3181 gttaatttag gttgagttat ataactcatt taaatacatt tggttatgta gcttctttaa
     3241 ttgaaaactc tttcatgtaa ctttaaatcg atcagcttgc tttcatcaaa aatctgtaca
     3301 tatgcttgta cataatgtac aaaacagcag aggtgttttg cgatttgtac aaggtgagta
     3361 atggcgctgt ctgatgtgtg gttgcgttca gtcgttggaa aggaacgtga taaggttttg
     3421 gttaaatccg atcgtgatgg tctgtctgtc agagtatcac cgaaaggtcg cgtagtgttc
     3481 caatatcgtt atcaatgggc agggaaaggt gagcgtcttg atatcggaac ttacccggca
     3541 actggattaa aagaggccag agaagaagtt atccgtcttc gtggtgaact cgagtcaaac
     3601 cgtaatccac gattggtcaa gcaggctgaa aaacgaaaag ttactgaagc catgacggta
     3661 gagtctgtga tccgtgcttg gtatgaagca tattgtgtaa aaaataaaaa aggttctgaa
     3721 cagatacttc gctcgtttga gctgcacctg ttctctaaaa tcgggaatat ccctcacgat
     3781 gcagctacat tgcatgattg gttagaagtc ttggagcctc ttagcactaa gactccagca
     3841 atagcagacc gattgctaat taacgcaaag caggcccatg tctgggcgta taagagaaag
     3901 ctcattgaaa ctcgcccgct gtcggatatc acgggtaaag atatggatat ccgtaaaggt
     3961 cagaagaaac ggtttctgac acatgatgaa attaaaatcc tttatgctgc gatcgatggt
     4021 tctcgaatgg ttcctaaata ccgggccttc attaaactat tgctgcattt tggttgccgt
     4081 agttcagagc taattaccgc ccgggtggac gattttgatt tcattaataa agtatggact
     4141 gtaccaccag aacgacataa gactggggag ataacaggcg aaccgctaaa gcggcccatt
     4201 attgaaccgg ttgaagagct tataaagtac gctatctcta tgaacaatgg ttccgatatg
     4261 ctttttacta aggaaggaag cagggagccc gttggtcgga catcattgca gtcgctgcct
     4321 tacaatttaa tgcagtacgc atggcggtgt ttaggatatc aattccctca ttggtctctt
     4381 catgacttga gacgtacagc tcgaacaaat ttttctgatc ttactgcgcc tcatatcgca
     4441 gaaattatgc ttggtcataa gctgccaggg gtatggcaag tttatgataa gagtgattat
     4501 ctagaagaac agcgtaaagc ttacctggca tggtggaaga gggttgaatc gattgttaat
     4561 tgtactatct ctgattcaaa ctgacggttt gtatcacctg aacataacca aatctcctag
     4621 cacactttga gcgagatgtg agcgttaagt ttacgccatt gtgttgacat aagagggggc
     4681 gggtatcgat atcattacta tccctatgcc cctaaatcgc tcctgccatt tgataaatgc
     4741 agatgtttac caccaaatgt ttttgatact ataggtcgat tcatgattta cctagaaact
     4801 tagttgtaac gaaaacttct taacggacac tatctggata aagttagttt tttatttaag
     4861 agggttaaca tgtcaaagtt aaaaaatgaa ccattagtaa gggtatcaga agaagacgga
     4921 atagaaataa ggaagatcca atatccagat aatacgatag aaagaattta taagcagaaa
     4981 ggtgtaatat taccacgaat acctt
//
LOCUS       DAZGBC010000255         4951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGBC010000255 DAZGBC010000000
VERSION     DAZGBC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.257"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..552
                     /locus_tag="ACHK1J_004785"
     CDS             <1..552
                     /locus_tag="ACHK1J_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913942.1"
                     /translation="LIGEIDNKLHSEKLIHKHHVTDAINTIKYLEILFTSSNLKRYKN
                     KEHYIKHIVAKSLLPKAEEIIRIQKDEFSSHYEHLKQTVSEIPNHKDIYNHINNENKK
                     IKIPVLISPVVMMMISLITLTDNIIKQQRILYLCGEISKKDFYSNRRVVLKKFSNVRL
                     KLHHNRKEYSEKLSTLIEKKGMQ"
     gene            complement(1120..1470)
                     /locus_tag="ACHK1J_004786"
     CDS             complement(1120..1470)
                     /locus_tag="ACHK1J_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913943.1"
                     /translation="MKDNQNQSLQVGDATIRIGDIFHTHWGYEQTNIEFYQVVSLHGK
                     KTVGLREIAREVVEHTSWCSAEIRPVPDKFIDEAIHKRRVIAKWGSVLVRFSDVTNAW
                     RTSPEALHHCSWGY"
     gene            complement(1657..2160)
                     /locus_tag="ACHK1J_004787"
     CDS             complement(1657..2160)
                     /locus_tag="ACHK1J_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913944.1"
                     /translation="MHQEQDIADALHESLAYFNRYCRYSLKRLAADYQEEIDNYQDDV
                     WEAPQRAARLSAAVKNYKTSRMIEFIFYMAIKLNLDLTPLVVKRLCSCLFQRTGSQEL
                     IVEIFGKKQRVNRSADNTMERINDVTERYRKSATRHWRKTLSDIEKVKKGYQKEIRAR
                     RKKKSGK"
     gene            2532..2831
                     /locus_tag="ACHK1J_004788"
     CDS             2532..2831
                     /locus_tag="ACHK1J_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7913945.1"
                     /translation="MATYRELLAKESPEMQARVAEQVEKASIQLALSALRDELNISQT
                     ELATMMGVKQPSVARMEQTDNDPRISTLKRYVKALGGELSLDVTLPTGKRVAFHL"
     gene            2850..3140
                     /locus_tag="ACHK1J_004789"
     CDS             2850..3140
                     /locus_tag="ACHK1J_004789"
                     /inference="COORDINATES: protein motif:HMM:NF036629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFO7913946.1"
                     /translation="MRTLEEVIAAQSPESQAIIKAMADKMRLEIGVKTMNTVQIPTGT
                     IKTFGLLGIPYEVGEPAQELPDGDILVNIILVQTGEKELYKLSKLLQDPDAE"
     gene            3144..3422
                     /locus_tag="ACHK1J_004790"
     CDS             3144..3422
                     /locus_tag="ACHK1J_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFO7913947.1"
                     /translation="MYAISFDLTVADTEKHHPKGVSQAYNDISSTLKRYGFERIQGSL
                     YTNSDENMANLFQAMNALKALEWFPNSVRDIRAFRIEQWSDFTSSIKG"
     gene            complement(3568..4059)
                     /gene="yjeJ"
                     /locus_tag="ACHK1J_004791"
     CDS             complement(3568..4059)
                     /gene="yjeJ"
                     /locus_tag="ACHK1J_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7913948.1"
                     /translation="MEYTPIALSPGAMLHKGKFIGYVMKIRFSECSGDHLFYLRYDMV
                     VELARALARKTHQMYLKGTFSTDESSQATLEMEKDYKQHDIYLTSDEISSPSVNNFVA
                     LLDYNVRDASLLLSLIKNDNSKIDISIPEAAVEIFTGYMMNTVIKAGGEALSEKLISD
                     IGV"
     gene            complement(4088..4288)
                     /locus_tag="ACHK1J_004792"
     CDS             complement(4088..4288)
                     /locus_tag="ACHK1J_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="HFO7913949.1"
                     /translation="MKLGNHKATNTALDALKKPAGATRKLQMNIPEELHLAFKLACVE
                     DGVDMTQVNIELIQNWLKSRNL"
     gene            complement(4294..4917)
                     /locus_tag="ACHK1J_004793"
     CDS             complement(4294..4917)
                     /locus_tag="ACHK1J_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7913950.1"
                     /translation="MPKVIAFLNGKGGVSKTTSAVNIATALARKGYKVVVVDTDPQGS
                     VSNWYDDSKCVFDVAEATTEKEIYRLRKALPLHDFIIIDGAAAISAISAAAVMVSDLV
                     IIPVTPSPLDFAACGAILAVIEARQDLRPVIARFLITKKVQQTHMLSLLKESIAETSI
                     PALKTGTSYRQSYIKSMLDGGTIFDTKDSAAKGEISVITEEILGLLK"
BASE COUNT         1589 a         1079 c          873 g         1410 t
ORIGIN      
        1 cttattggag aaattgacaa caaattacat agtgaaaaac ttattcacaa gcatcatgtc
       61 accgatgcga ttaatacaat taaatatctt gaaattcttt ttacatcaag caacctaaaa
      121 cgttataaaa acaaagagca ctatataaaa catatagttg caaaatcatt attgccaaaa
      181 gcagaagaaa ttatccgtat tcaaaaagat gagttttcgt cgcattacga acacttaaaa
      241 caaactgttt cagaaatacc aaatcataaa gatatataca accatataaa taatgaaaac
      301 aaaaaaataa aaatacctgt tcttatatca cctgttgtta tgatgatgat atcccttatc
      361 acccttacag ataacatcat taagcaacaa cggattcttt atctttgtgg agaaatatca
      421 aaaaaggact tctatagcaa tagaagagtt gttcttaaaa agttttctaa cgttaggcta
      481 aaattacatc ataatcgaaa agaatacagc gaaaaacttt caacactgat agaaaaaaag
      541 ggaatgcaat agcattccct tacagttcag aatcagatca ataacggtct agattctgac
      601 gaatctgaca aatgaaatat tcggcttcaa actccgtaat ctcatttgtc acgagcgagt
      661 atacaatcaa atgaacaata cctgctcgta gatcaattac agcatgaata actgccatac
      721 tcctgaaaag atatgttctc aataatcgag aatacctatc attttcgcga agattgcatt
      781 cattcagcat accatcagct tgagcctcaa caatgaaatc agtgaacgta tcaacagaaa
      841 caatcgactg atagcatcgt tctcccatta cagaatcaaa tggagatctg caagggatca
      901 ggtttaacga tgataaattg tttataacac tagtcataac cagactcctg tattcagaag
      961 ctgcgttacg ctgccgaagc aacgtaacgt tgcttcgaca gttgtgtgaa tgttctttga
     1021 tgctgaaacg tcagaacatc aaaattgatg ctgcatcatc tgacagaacc aatctatcac
     1081 gcataaagaa gaaggcgcaa ctgcgccttc tcacaacgtt tagtatcccc aggaacaatg
     1141 gtgtaaagct tctggtgaag ttcgccatgc attggttaca tcactgaacc gtacaaggac
     1201 agacccccat tttgcgataa cccggcgttt atggatggct tcatcaataa acttatcagg
     1261 aacagggcga atctctgcac tacaccaact ggtgtgctct acgacttccc tagcaatctc
     1321 ccgaagccct actgttttct ttccatgtag tgaaacaact tgataaaact cgatattagt
     1381 ttgctcatag ccccaatggg tatgaaaaat gtcaccaata cgaatagttg cgtcacctac
     1441 ctggagtgat tggttttgat tgtctttcat aacgtttacc tcaaacatga ggatcacgtt
     1501 acgctgccag aggcaacgta acgttgcctc gacagagaat tggttaatga taggttacag
     1561 tctgcccttc cctatcccgc agacagaaaa aagatacact aatgattaaa agatatccac
     1621 tctcccccca caacctttgt agtgagtgag ttcacttcat ttaccgcttt tcttttttct
     1681 ccgtgcacgt atttctttct gatagccttt tttaactttt tctatatctg ataatgtctt
     1741 acgccaatgt cttgtggccg atttacgata acgttcagtc acgtcattga ttcgttccat
     1801 tgtattatca gcactacgat ttacacgctg cttttttcca aaaatctcaa caataagctc
     1861 ctggctacca gtacgctgaa aaagacaaga acataaacgc ttaacaacca gcggggttaa
     1921 atcaagattg agctttatag ccatataaaa aataaactca atcatccgtg aggttttata
     1981 gttttttacc gctgcactta accgcgctgc gcgctgcgga gcctcccata catcatcctg
     2041 gtagttatca atctcttcct gataatccgc agcaagcctt tttagcgaat agcggcaata
     2101 tcgattgaaa taggctaggg attcatgaag tgcatctgct atatcctgct cttggtgcat
     2161 actacgccct atgctaacgc tactatatgt acacattatg cattagaact tgtacaaaac
     2221 aatcaatccc cagtctcggc tacctccctt caccggataa cttgcaaata taaggctttc
     2281 acatccaaat tcagccaaaa caagaaacga gattttatta acaaataagc aacataaaaa
     2341 acgacgaaaa acgcccagga aaacatgatc ttttgagcgg atttttcaga tctgacaacg
     2401 aactgtgatc cggtggtgct gataacgtcc attatgttaa atagccctgt ttttctggaa
     2461 attatagtgt attcgttata atgcagtata taatgaatac cgtgaacatc ttgcaaaact
     2521 ggagaaataa catggctacc tacagagaac tactggctaa agagagtcct gaaatgcagg
     2581 cgcgcgtagc cgagcaagtt gaaaaagcca gcattcagct cgcacttagt gctttacgtg
     2641 atgagctgaa catatcccaa accgaactgg ctacaatgat gggagtaaaa caaccatcag
     2701 ttgccaggat ggagcaaact gacaacgatc cgagaatatc gacgcttaaa cgttatgtaa
     2761 aagctttggg cggcgagtta agcctggacg tgacattgcc tacaggcaag cgtgtggctt
     2821 tccacctgta aaaaattggg catcaacaga tgagaacatt agaagaagta attgcggccc
     2881 agtcacctga aagtcaggct attatcaaag ctatggctga taagatgagg ctggaaatcg
     2941 gagtgaaaac aatgaataca gtgcagattc caacaggtac gattaagaca ttcgggctgc
     3001 ttggtattcc ttacgaggta ggagagccag cccaggaact acccgatggt gatattcttg
     3061 tcaacattat actggttcag acaggtgaga aagagttgta caaactttca aaactattac
     3121 aagatcctga tgcagagtaa gttatgtacg caatatcatt tgatttaacc gttgcagata
     3181 cagaaaaaca tcatccgaaa ggcgtgtcac aggcttacaa cgatataagt tcgaccctta
     3241 aaaggtacgg atttgagcga atccagggaa gcctgtacac aaatagcgat gaaaatatgg
     3301 caaacctttt tcaggctatg aacgcattaa aagctcttga atggttccca aattcagtca
     3361 gggatatcag agcattcagg attgaacagt ggtctgactt cacttcgtct ataaagggtt
     3421 gatatcctcc acgccctaat gtcgtggagg acggaaggtt aagcctggat gttgccccct
     3481 accggaaagc gagtggcctt taagatttag ttcaaaaggg gcgaaaagcc ccttttttga
     3541 gcgttcgcgt ttacctggca gcaataatta aactccaata tctgatataa gtttttcaga
     3601 taaagcctct ccccctgcct taataacagt attcatcata taacctgtaa atatttccac
     3661 cgctgcttct ggtatactaa tatcaatttt agaattatca tttttgatta gtgataataa
     3721 taatgatgca tcccttacat tataatctaa caatgcaaca aagttattta cagatggtga
     3781 cgatatttca tcacttgtta aataaatatc atgctgctta taatcctttt ccatttctaa
     3841 cgtagcctga ctactttcat ctgtactaaa agtcccttta agatacatct ggtgtgtttt
     3901 tcttgctaat gctcttgcaa gttcaacaac catatcatat ctcaaataaa aaagatgatc
     3961 tccagaacac tcggaaaatc tgatcttcat gacataacca atgaatttcc ctttatgcaa
     4021 catagctcct ggacttaacg ctattggtgt atactccata aatcctccat atgcatcata
     4081 acaaaaccta taagttacga ctcttcaacc agttttgaat tagttctata tttacctgtg
     4141 tcatatcgac accatcttcc acacaagcta acttaaatgc aaggtgtaac tcttctggaa
     4201 tattcatttg taatttcctg gttgctccag caggtttctt tagtgcatcc agagcggtgt
     4261 ttgttgcctt atgattccca agtttcattt ttctcatttt aatagtccaa gtatttcttc
     4321 tgtaatgact gatatttcac ctttagcagc actatctttt gtatcaaata tagtaccacc
     4381 atctagcata gactttatat atgactgcct gtacgacgtt cctgttttta atgctggtat
     4441 gcttgtttct gcaatagatt ctttcagcaa agatagcata tgcgtttgct gtactttttt
     4501 agtaatcaaa aagcgagcaa tcaccgggcg taaatcctgc cgagcttcta tcactgccaa
     4561 tatcgcgcca catgcagcaa aatcaagcgg cgaaggagta actgggataa tgacaagatc
     4621 agaaaccata acagcagctg ccgatatcgc cgaaatcgcc gcagcaccat caataatgat
     4681 aaaatcgtgc aaaggaagcg ccttccgcaa acggtaaatt tctttttctg tcgtagcttc
     4741 tgctacatca aaaacacact tactgtcatc ataccagtta ctaacacttc cctgaggatc
     4801 ggtatccaca acgacaactt tgtatccctt acgagcaaga gcagtagcaa tattgactgc
     4861 cgaggttgtc ttagacaccc cccccttacc atttaaaaat gctataactt taggcatttg
     4921 cccctcccct ttgttcacat agtacctctg c
//
LOCUS       DAZGBC010000256         4936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGBC010000256 DAZGBC010000000
VERSION     DAZGBC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.258"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..479)
                     /locus_tag="ACHK1J_004794"
     CDS             complement(<1..479)
                     /locus_tag="ACHK1J_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7913951.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSALTAMTASFVLATPTETDG
                     "
     gene            complement(479..808)
                     /locus_tag="ACHK1J_004795"
     CDS             complement(479..808)
                     /locus_tag="ACHK1J_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7913952.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKRY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(805..3366)
                     /locus_tag="ACHK1J_004796"
     CDS             complement(805..3366)
                     /locus_tag="ACHK1J_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7913953.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMTRVRRHFSGLETDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRMLPMQFTDVATQLAGGQNPFLIMLQQGGQVKD
                     SFGGMIPMFRGLAGAISLPMAGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTAERMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDSINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQAELNKALRDGKILQADYNTLMASAKKDYEST
                     QKKPKSSGVRVSAGERQEDRAHAALLALETELRTLEKHSGANEKISQQRRDLWKAESQ
                     YAVLKEAATKRQLSGQEKSLLAHEKETLEYKRQLAELGDKVEYQKRLNELAQQAARFE
                     EQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQALARVTGALKQTWADEDM
                     LRGDWLAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWRGFTR
                     SVLSMMTEILLKQAMVGMVGRIGSAIAGAFGGGASASSGTAIEAAAANFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(3347..3760)
                     /locus_tag="ACHK1J_004797"
     CDS             complement(3347..3760)
                     /locus_tag="ACHK1J_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7913954.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRREEAQTERPDEEDMLMQKAAGLAGGVRFGEEGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            complement(3787..4218)
                     /locus_tag="ACHK1J_004798"
     CDS             complement(3787..4218)
                     /locus_tag="ACHK1J_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7913955.1"
                     /translation="MFLKTEQFEYNGVSVMLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMSEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNVLTDDDFSVGKSSTAS"
     gene            complement(4235..>4936)
                     /locus_tag="ACHK1J_004799"
     CDS             complement(4235..>4936)
                     /locus_tag="ACHK1J_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7913956.1"
                     /translation="LWVYTGKGDAYANPLSDDEWTRLAKIKDLTPGEMTAEPYDDNYL
                     DDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDSSETRAYKIRFPNGTVD
                     VFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVAKG
                     QSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGNGE
                     FAAVAEITVTDGAAG"
BASE COUNT          966 a         1678 c         1177 g         1115 t
ORIGIN      
        1 ccgtccgtct ccgtcggtgt cgccagcaca aacgaggccg tcatggccgt cagcgctgat
       61 aactgctcca ccacccaccg gtccgtcagc tcctgctccg ggtctgcctc cggattcccc
      121 gccacaaagt tcaccgcatc aagaaaacgc gcatacaccc ggcggcggac caccgtggca
      181 cccaccaggc tctgcaaatc ctccgccatc ccggtgacaa ggccgaaaag attggacacc
      241 gtcagcgacg ggcgggcact gctgcccttt ccgttcatct caaagccgct gccctcaatc
      301 gggtacgcct catacttccg cccctgccag gtcaccggct cccctttttc attcagctca
      361 ttgcagaaaa aataccgctc accgccctgc accgtcaggt cgatttccca gagcaccacc
      421 cgcggtgact gctctgactt aaccgactcg ttcaggcttt cttcgtgaat atcctgcatc
      481 agttcaccac ctgcttaaac tccgcgctga actcaacgcg caacatcccg acccgcgcag
      541 accacccggc gcaggtcacc tttatctgcc ggtatgcata gggtggcttc cacaaaaatg
      601 ccttccagcc cccgtgctct gccagaaacg cttccagatg ccgggcctcc tcccgggtca
      661 cggaaagcat caccctgtac ctcttcaggt ctgcattcag ccctgccgcc atacgctgtg
      721 agtacccgtc accaaaacgc acttcacgca ccgacggctg cgagttcacc tccatatccg
      781 gcttcacttt ccagcgaaag gttttcatcg cccgctccct gataacatac cgccatcacg
      841 caactgcagc cggagttcat cctgcgcccc cttacgggcc atgtcataca ccgccttcat
      901 cagctgcggc cccgcctgtc cgtttatacc gtcgttctga atcaccacgt gattgttctg
      961 attaaaatta ataccttccg cccgccgcat ctgcgccgga cttccggcac cacccacata
     1021 acccccttcc gcatagccgc gcatcaggcg gtaaagattc cccacaccta tccggctggt
     1081 tgcctccttc gtgaagacaa actccccgcg gtgaactatc cccgcaggct catatttgcc
     1141 gcccgtcccc gtaaatcctc cggtcgcgaa atggaagttc gccgccgcag cctcaatggc
     1201 cgtccccgag gaagcagatg caccaccacc gaaagcacca gcaatggcgc tgccgatacg
     1261 cccgaccatg cccaccatgg cctgtttaag caggatttct gtcatcatgg acagcaccga
     1321 acgggtgaat ccccgccagt tctgttcact gccggtcagc atcgccgcca tattctgtgc
     1381 aataccgtca aaggtctgcg tggccacact tttaacctgc gaaaaactgt ccgtcgcact
     1441 ttccgcccac tccccccagc cggacttcag cccggccagc cagtcaccgc gcagcatgtc
     1501 ttcatccgcc catgtctgtt tcagtgcccc ggtgacccgg gccagcgcct gcggattatc
     1561 gccatacacg tcacgaagac gctgcgcttc agactcccgc tgcgcctgac ggtcagtgag
     1621 accacgggct tttgcgctga tggcggcctg cttcgcgctc tgctgctctt caaaccgtgc
     1681 cgcctgctgt gccagctcat tcagccgttt ctggtattca accttgtcgc ccagctcagc
     1741 cagctgacgt ttgtactcca gcgtctcttt ctcatgggcc agcagggatt tttcctgccc
     1801 ggataactgt cgtttcgtgg cagcctcttt caggaccgca tactgacttt ccgctttcca
     1861 taaatcacgg cgctgctggc tgattttctc attcgcaccg ctgtgcttct ccagcgtccg
     1921 gagctcagtt tcaagcgcca gcagggctgc atgcgcccgg tcttcctgac gctccccggc
     1981 tgacactctg acacctgacg acttcggttt tttctgcgtt gattcataat cctttttcgc
     2041 cgacgccatc agcgtgttgt aatccgcctg caggattttc ccgtctctca gagccttgtt
     2101 cagttctgcc tgacgggcgg tatatttctc cagcggtgtc tgcaggcgtt cgtaagcctt
     2161 ctgcgcctct tcggtatatt tcagccgtga cgcttcggta tcgctctgct gctgcgcatt
     2221 tttgtcctgt tgagtctgct gctcagcctt ctttcgggcg gcttcaagcg caagacgggc
     2281 cttttcacga tcatcccagt aacgcgcccg cgcttcatcg ttaacaaaat aatcatcctt
     2341 gcggagattc cagatgtcgt ctgctttctt aaacgcagtc tctgccttaa tcagcatctc
     2401 ctgcgcggta tcaggacgac caatatccag aatggcatcc cacatcgatt tgaacgcctt
     2461 ccctgtttta tccgcccagg tttccagcgt ccccatgttc tctttcaggc ggcgggtctg
     2521 gtcatcaaag cctttcgtgg cagcctcgtt cgccgcctgc aatgccccgg cctcgtctcc
     2581 ggaacgctgc agctgcgcaa catacgcaat ctgctctgcc gtcacgttac ggaactggcg
     2641 cgccatcgcc atcagtcccg acgtcgggtc ggtggtcagt tttccgaaag cctctgcaac
     2701 cttgtccact tccacaccgg aggcagacgc aaaacgcgcg acactctggt tgatggaatc
     2761 aaactgttca ccaccacgca caccggcatt caccagggct gccagtgact cactcgcctg
     2821 gttaaacgtc agccctgctg cctgtccggc tctggagagc gtaagcatgc gctctgccgt
     2881 cagtcctgac tgattaccgg aaagaaccag ggttttatta aacgctgaaa gcgtggaatc
     2941 cccctggtac caggcgtacg ccagcgcacc ggtcgccacc gccagcgagg tgacaccggc
     3001 cattggcagg ctgatcgcac cagcaagccc ccggaacatg gggatcatcc cgccgaagga
     3061 gtccttcacc tgaccgccct gctgcagcat gataaggaag ggattctgac caccggcaag
     3121 ctgcgtggcc acgtcggtga actgcatggg tagcatacgc atggcggctt tatactgccc
     3181 gactgatatc ccggcttttt gtgcagccag cgcctggcgg ctcagcccct gctcaacagc
     3241 actggcggtt tttctggcgt cagtctccag tcctgaaaaa tgacgcctta cgcgggtcat
     3301 ctgctcatcg aaacgaaccg catccagact caggtcaata acaagatcac caaccggctg
     3361 ggacatatct cacacctccg gaaatccccg ctgaagccat cattaatgcg acatcatcct
     3421 cgctgacatc caccacatcc gcagaaggtg aaatatcgcc ccctccctcc tcgccgaacc
     3481 ggacgcctcc ggcaagtcct gccgctttct gcatcagcat gtcttcctca tccggcctct
     3541 ccgtctgcgc ttcctcacgc cgggggacga gcagactgaa atccgaggga tgcatatccg
     3601 gatcgcaaaa aaacaggctg agtacggcgt acgtcagccc ggaaaaatgc atatccagct
     3661 gggtatcgtg aaaataatgc gtgcggtaaa aacgtcgcca gtcggcatat tcggtggatg
     3721 tcatcccggc aagcatggcg cgccagtcgg gtctccccat ctcacgcgcc agtctgaggg
     3781 caaaattcag ctcgccgtcg aagactttcc cacagaaaaa tcatcatcag tcagcacgtt
     3841 atttttcgcc acttcggtga tatcagtatc cggacgaaca gcttcgatca tcccggacag
     3901 gcacaacaca acatcttccg cccgggcaat ggcatcggca ggccaggtgg tgagcacttc
     3961 ctgctctatc ttcatcacgg cctcactcat tgacggtgac tgcgttttct gtggatggtt
     4021 atgccacagg gacatcgcca ccagaaacgc gccggttctg acaagatctt ccacactcac
     4081 ctgcaggttg ccgctggctt cagcctcttc tgcccgccgt ttcaggaggg caagatgctc
     4141 aatacgctgc agcgcagaca gctcagaaag catgacggat acaccgttat attcaaattg
     4201 ttctgttttc aggaacatgc ttgatctcct cccctcagcc cgcagcgcca tccgtgacgg
     4261 tgatctccgc caccgccgca aactcaccgt tgccggtgac aacagggatc tgcgctttac
     4321 ctgccgcaac acctttcacg gtgatcgtgt tccctttcac ggtaatggtg gcgaaattct
     4381 gatttgccga cgttgcgcgg aatgttttat ccgttgcgcc ttccggctga acggccacag
     4441 tcagggtggt gctctgacct tttgccacat tccccgttag tggcgtcacg gtaataccgg
     4501 tgaccggtgt gatttctccc cgatcctccg ccagcgaagg acgaccgata ttggtgattt
     4561 ttaccgtacg ggtaatcact tctttggcgg tcaccgcttt accaatggcg ctcacccagc
     4621 cacggaacac atccaccgta ccgttcggga agcggatttt gtaggcccgg gtctcactgc
     4681 tgtcaaacca ggcaatcaaa tcacgctgcc ctttctcgcc gggcttccag gccagcgtaa
     4741 aactggtgtc accggcggat ttctgcccct gcccggtgga tacccagtcg gcatcctcat
     4801 catccagata gttatcgtcg tagggttccg ccgtcatctc gccgggggtc agatccttta
     4861 tttttgccag acgcgtccac tcatcatctg acagcgggtt tgcataagca tcgcccttac
     4921 cggtgtaaac ccagag
//
LOCUS       DAZGBC010000257         4829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGBC010000257 DAZGBC010000000
VERSION     DAZGBC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.259"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..774
                     /gene="slp"
                     /locus_tag="ACHK1J_004800"
     CDS             208..774
                     /gene="slp"
                     /locus_tag="ACHK1J_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO7913957.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            930..1460
                     /locus_tag="ACHK1J_004801"
     CDS             930..1460
                     /locus_tag="ACHK1J_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7913958.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1521..2549)
                     /gene="chuS"
                     /locus_tag="ACHK1J_004802"
     CDS             complement(1521..2549)
                     /gene="chuS"
                     /locus_tag="ACHK1J_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO7913959.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAV"
     gene            complement(2598..4580)
                     /gene="chuA"
                     /locus_tag="ACHK1J_004803"
                     /gene_synonym="shuA"
     CDS             complement(2598..4580)
                     /gene="chuA"
                     /locus_tag="ACHK1J_004803"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO7913960.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     IDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1290 a         1169 c         1095 g         1275 t
ORIGIN      
        1 gattttatgt aattttaaaa caaagggtta tattatgggg cgatggatac tcatgccacg
       61 ccccaaaata acggaattga actaaacaga agggatataa acatcagaca ggtttacgtt
      121 actatcaggc atatcgcctc agaatcagat gaaaactata aagaaatatc tattatggtt
      181 ttaatatttg ttgataagga tagtaacatg aacatgacaa aaggtgcact catactcagc
      241 ctttcatttt tgcttgccgc atgtagttca attccgcaaa atatcaaagg caataaccaa
      301 cctgatattc aaaaaagttt tgttgctgtt cataaccagc cggggttata tgttggtcaa
      361 caagcgcgct ttggtgggaa ggttatcaac gttatcaatg gcaaaacgga tacgttgtta
      421 gaaatcgctg tattaccgtt ggatagctat gcgaagcctg atattgaagc caactatcag
      481 ggccgactgc tcgccagaca aagcggcttc cttgatccag tgaactatcg taatcacttt
      541 gttaccatcc tcggcaccat tcagggtgaa caacctggct ttatcaataa agtcccgtat
      601 aacttcctgg aagtgaatat gcagggcatc caggtgtggc atttgagaga agtggttaat
      661 accacctata acctgtggga ttacggctat ggtgcattct ggccggaacc gggctggggt
      721 gcgccttact acaccaatgc ggtgagtcag gtaacacctg agctggtcaa ataacaccac
      781 cgaaagatgc agatgctttc tcagcatctg catcatgtat tacataaaat taatatacag
      841 taagctaact attattatta taagccctgt cctgttaatt acctttggca aactgattat
      901 aaagttaatg tccgcaccag gagtcggtta tgtttcttat aattaccagg gatacgatgt
      961 tcttcaccgc gatgaaaaac attctgagta aaggtaatgt cgttcatata cagaacgaag
     1021 aagagatcga cgtaatgttg catcagaatg ccttcgtcat tattgataca ttaatgaata
     1081 atgtatttca ttctaatttt ctcactcaaa ttgaacgatt aaaacctgtc catgtcatta
     1141 ttttctcccc ctttaatatt aaacgctgcc tggggaaagt gccggtgacc tttgttccac
     1201 ggactatcac tatcattgat tttgtcgcac tcatcaatgg cagttactgc tctgtgcctg
     1261 aagcgaatgt gtcactttcg cgcaagcaac atcaggttct gagctgcatt gcgaatcaaa
     1321 tgacaacgga agatattctg gagaaactga aaatatcgct aaaaacgttc tactgccata
     1381 aacacaatat catgatgatc ctcaatctta agcggatcaa tgagctggta cgccatcagc
     1441 atattgatta tctggtgtga acgattgatc aatataaaga ggcccagcaa taaatagcca
     1501 gacctcccgt taattatacg ttatacagca acgccttccg gtatcagcga agcaatttgc
     1561 ttacgccatt gcgcttgctc ctgttcgcct tctgtacgtt gaccataaag ttgcgctatc
     1621 tgcgtaccat catgggcaaa caattccaga ctggttacgt agccatcgct ggtcggttta
     1681 cgggtaaccc aggcttcagc aatgctctct tctaatagat gaagagtaaa cgtcgggttg
     1741 aaaatattca gccaaccttt cattggcacc actttttcta ccacaccggt gaaaatctgt
     1801 acgcagccac ggttgccaac aaacaccatg atttcattac catcctgctg tgcagattca
     1861 agaatttgcg ccaacgcact gttggatact ttgcaggcca aatcgtctgc caccagattg
     1921 aacgcctgtt ggcgcgtcag gttgtggcgc ttgagcaacg taaaaaactg atgaacgtcg
     1981 gtcatcgccc gccactcttg ctcgaccaca gtggcatcgg ctcgcgtttg aacaactggc
     2041 gcatcaacgg cttttaactc aagcggcgta ttctcatcgg tgataaaccg tgccagaagc
     2101 tcactccatg ccgccatatc ggtattgtcg gtggcataaa cttttagtaa tgcatcgccc
     2161 tgatgatcaa agaactgaat actctggcgt tcgccacgag ccgtgttttc tttgatgtgg
     2221 aaaacactgg cccattgatt gagaaacaga cgtaaatcca gcgcgcgcgg attgaggatc
     2281 aatccggcat gtccgttcaa atgctggttt gtgaacgtac caacttgctc atggactgca
     2341 tattcattac gacaaataca tttggtttcg ccaacacttt cgagcgccgc cagaatttca
     2401 cggatatcgc cgtgcatccg ccacgcatcg tgcgtgacgc gtgcaaatgc cagttctgct
     2461 tcgctgatat tcattaaacc tgcgatgtca cgcgcgtact ttccgggatt ttgttctttt
     2521 aactcaagcc agcgtgtgta gtggttcatt gtctctcttc cttccagata aatgccccga
     2581 aatatcgggg cagatgatta ccattgataa ctcacgaaaa tttttccgtt acgaccatcc
     2641 tgtgggatgc cttgcggcga ccagtactct ttgtcgaaag cgttacccaa caccaaagta
     2701 gtggtcatac ctttgagcgc ctgttgtcct tgataactga cgtagaaatc attcacgcca
     2761 tagcctggtt gtttgctgta actgctgctg atatgtgttg agcgatcggc aaacgtacca
     2821 acccacccaa cagagaagcc actgtgagcg atcggaatat tcagagtgct ggtaacggta
     2881 tccgggttaa tgctggagat gtattcgccg gtatcggtgt ctttgccgcg ggtacggtta
     2941 taggccacat caaggctaaa cagatcagtg gtatatttcg tcatcacatc ccagccccag
     3001 attttggcgt tcgggacgtt atacgacata gtcgtcgccg ccgcgaaatc gacggtcgtg
     3061 gagatgtaat ccttcgcttt ggtatcaaag tagctggctt taaattccag agcatcattg
     3121 gacaacatca ggtcatcaaa acgcagccca aaaccgtact cctgagtttc gttagtttcc
     3181 ggacgtaagt tcgggtttgg cacccaatag ttggtataga agcgaccaat cgagaagtgc
     3241 ttagaatcgt tatacatttc gcccatcgtc ggggcgcgga atgcctgggc atatgagcca
     3301 aataacatca gccagttagt cggattgata gtcatccccg cacgagatga ccatttgtcg
     3361 gcatcaacat ctttgtaacc gtcactgcta ccgcgataac tgtcatagcg ggttccgcca
     3421 agcagggtaa tcggcagatc gcgtaaggtg atctcatcct gtagccagcc ggagctaaaa
     3481 tcgatttttg cttgcgggaa gcccgtcgtc gcgccgcccg gatgttgttc ctgacgataa
     3541 tactcaccgc catatgtcag taagtgagaa gcgaaactgt cggcaaagag agtggaacgg
     3601 ttctccagcc tggctccttt tgttatctgt tcacgatact cgccggaact ccccgtgttt
     3661 tgcgcattaa tacggacttc cgaccaataa atttttgcat ctgcatttaa ccagtcgttg
     3721 ccctgcgggg cgagtttata agaaagctgc gcatcgcgtt gaattgttga acgatcaacc
     3781 atcgggttgc tgctttcaga agcctcaacg gtctgcggat tttttggttc acgcgcgtcg
     3841 ttgttgtagt aacgcactaa accgctcaga gactgggctg aatcaatttg ccaggtccct
     3901 ttcgccagca tgttattaat ggactcgtca ttcggcgcgg tttcaccatt gctctggcgt
     3961 aaatcacccc gatcgcgact ggaccaggcc acaataccat ccagattttc agttcgccca
     4021 aacgcgctcg cgcctaatcc caggctatgg tcccccgtgc cgccagtacc aaagacacga
     4081 aaaccactgc tttgtccttc ctgcaataaa tcttttgcat cgaccgtatc gtaggatatc
     4141 actccaccca gcgcgccact gccatacagt aatgctgaag gtccgcgaac aatctcaaca
     4201 cgcttgatca gcgccggatc gagaaaagtg ccattcaggt gtccggtatc cgttccctgg
     4261 cgaacaccat cgataagaac cagcacgccg cgatgatcat agccacgcat atttacatcc
     4321 tgaccgttgg ttcgtccggt accatccaga gtaattccag gaacatgacg cagcagatcg
     4381 gtggctgaag tagccgtttg attttcagga gcggaagtgt cgatgacgct gaccatcata
     4441 ggcgcttcga aggaactacg ggcattcccc gttgccgtaa cggtcatggt ttcagtagca
     4501 aaagcaaacg ttggcaaggt ggcagaaaca gccaaggcca ataaactcaa acgcaacgag
     4561 gtaaattgcg gacgtgacat agcgattctc catgaggaaa ataaaaaatg ctggctgcct
     4621 gggttaatac cggggtggct ggcgaaaaat aaatttattt aaaattcaga gcgataatat
     4681 tcgccgggtg agtttacagg cgtttatatc ccatgaaatt gcctgataac tcggcaatta
     4741 cgcaaaagca cgccctcttt gtcaggcaaa acgatatgcc ccatcaggag tgaagagagc
     4801 tgtgcgttat cccttgcaaa tgctatggt
//
LOCUS       DAZGBC010000258         4813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGBC010000258 DAZGBC010000000
VERSION     DAZGBC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.260"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..246)
                     /locus_tag="ACHK1J_004804"
     CDS             complement(<1..246)
                     /locus_tag="ACHK1J_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913961.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQ"
     gene            complement(527..705)
                     /locus_tag="ACHK1J_004805"
                     /pseudo
     CDS             complement(527..705)
                     /locus_tag="ACHK1J_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            complement(773..1046)
                     /locus_tag="ACHK1J_004806"
                     /pseudo
     CDS             complement(773..1046)
                     /locus_tag="ACHK1J_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1093..1659)
                     /locus_tag="ACHK1J_004807"
     CDS             complement(1093..1659)
                     /locus_tag="ACHK1J_004807"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7913962.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            complement(1687..1884)
                     /locus_tag="ACHK1J_004808"
     CDS             complement(1687..1884)
                     /locus_tag="ACHK1J_004808"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7913963.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
     gene            complement(2132..2557)
                     /locus_tag="ACHK1J_004809"
     CDS             complement(2132..2557)
                     /locus_tag="ACHK1J_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7913964.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDCGGAST"
     gene            complement(2554..2937)
                     /locus_tag="ACHK1J_004810"
     CDS             complement(2554..2937)
                     /locus_tag="ACHK1J_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7913965.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(3309..3902)
                     /locus_tag="ACHK1J_004811"
     CDS             complement(3309..3902)
                     /locus_tag="ACHK1J_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7913966.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRLDSTLSEICSLV"
BASE COUNT         1380 a         1182 c         1065 g         1186 t
ORIGIN      
        1 ttgttcccgc aaccagcgta ataccaccgg cgctgatggg ctgaccgtgt gatcacgctc
       61 cgtaatacgt tcgtgcaccc gtcggtgaaa ggaactacag aatgtctgaa tctgttgacc
      121 acagacgcct tctgtcgaat gaagtgtgaa gtggattgcc agcagatgcg gccagtgatc
      181 caccgcctgc tgaacaaaac gccggatttc ccccggctct gaaagtaagg cttcggttat
      241 ttgcactatt ttatctctgt tgaacttggt taagtcggtg cagacgcatc aacacaagta
      301 cggttcgatg caaacagctg tgactggcaa tatgaaagga atgatgaatc agtcaggatg
      361 acaaagtgcc agctgaccgg aggggacgca ggaagattca cggggggacc agcaccaggg
      421 aacagcgcca caataccaac gctgacacgt tgaacattgc cagcgtaccg gtatcacaac
      481 acgtttcata cttctgccac cgtgattctt cgattcgtta ctgtatctac tgtgacactt
      541 cgcttttata cctgcggctg gatcggcccg gcttgatgaa tcttcactga tcagcttata
      601 aacccctctg tcggtcatac cggtgaaact ggtgatatag ttcatgtcaa tcagggaatt
      661 atcggcacgc agaaatacgc tgtcgtggct tgttgtagtc aacatggtca gaatgtcctc
      721 tgtgagattt atgaagattg tgcgaatgcg gggaatctgc tgagctgtgc tttcagaact
      781 ggcctgttac gggataccag ggattaccgg cggggtaacg ggcttccgga tcatacacac
      841 cacgattatc acggacaaaa tcactgaacg cccatatcac ctctttaagt atgtcttcgc
      901 agtccggtac atgacgatcc agcgccacat cccgggtggt actactttga tgcgcccggt
      961 gacacaaagc ccggattgtt ccagacatcc tgaatcaaac gccccagatt aggggcgtcg
     1021 aaatatgcct ctctgaccat tatattccgg cgtacaggta gcaggtcaga agtgacaatg
     1081 cgtcacctga cgttaaaagt cactacaccc aagatgacgt tcaacagcac catgcgattc
     1141 aatgtaagcc cgggctgtct gttccagtac accaggctca gcgttgtatg tgttagctgc
     1201 atcaaatacc aacgacagca cttcaggata cacaaccaga tgtgtaatgg agttatcttc
     1261 acccaatact tttccccacg cctgctcaat cagatttctg agaaccacca cctcacgact
     1321 cttacaccag acatcgttat taagtagcag caccataaga taaggagtgg tatcgttagc
     1381 cacagcctcc ctactccaga gataatataa aggggtgggc tcaacagatt tatctttacg
     1441 tcgcttacac tgcaaatatt cagaaatgag tctatgcagt tcaccagtaa aatcagccat
     1501 caaagaggga atggccttat taataccctg ccctggtatt aatttaaatt gtaataattt
     1561 aatttcagga tgtgtggctg cagcccgata cagagttgca aggacacact tttgccagag
     1621 ggcgttacag gaaagcttaa catttgattc tgtatacata ataaatcacc ttacagttac
     1681 aacaggtcaa aaaccgctgt agccagagtt acgctggcct gatgctttag taccgggctt
     1741 cgtcagataa tccagacgct ccaataagcg ctgatactgc tcagggaaat caggatcatg
     1801 aatatccagg atgtaacgtc cattagcagg gaaatgaata atgcagcccc ctggattaac
     1861 aatgcagaaa tcgtcctgag gcactgatca atacggagag gactctcgcg tgtggtttat
     1921 tgacaccaca gtgcagattc ggcgaatccg cgatcacggt gcaatttcgt tccacagcac
     1981 acaatcataa ccccgggttt tattcaggta agcaggactg cggatatccg gtgtcgcgcc
     2041 tttgtgtcac gaacggggta ggtgcgaaac accggataaa atgcaggctg gcaatacctc
     2101 tgaacgccct gcgcagagcg gatattttgg attaagtact cgcacctccg cagtcctggc
     2161 acaaatctgg ctggtacctg taaacagact tcgtacatgt tgctctgcaa taaatccccg
     2221 taccacaggc ctcacagaat ttcttcccat gaaaaaactg ctcacacatc acacaatgcc
     2281 actccagcac gaccggtaat ggcgatagaa acatcgccat atcctcaatg taagggtggg
     2341 acttttccgg attcagcacc acgcaggtcg ccttctgttg cgcgctcagg gcatgtgaat
     2401 catgctcaaa ccacgccccc tgagcatctg tctgcaaaat caaccgacca cgacaggaaa
     2461 ggcagaaaca ctgccggata tttctgctga gcgcagagtc ggcactgata atgtgttcac
     2521 ccggcgtgat ccccagcccc gtttttatac cgttcattca gccactccct cctcactgaa
     2581 gtgccctgta tggcagtgtg tgcagtaccg ctccccataa taatcgtggt gacattgtct
     2641 gcagcgccag ctggctttac gcaccacggg taaggcatcc gggacgaatt gctgcagacg
     2701 tttaatcaac cgtatttctc tgcgttccgg cctgacataa ggacattgct gcgcgtgctc
     2761 cgtcagccct tcgtctgcgt gttcaaacca gggacgctca gtgtcgtatt gcgggtggta
     2821 tctgagcgca ctgccgcaaa ggtggcaagt gtagcgatcg taaggtgcag tctgtgcggt
     2881 acgggcaccc gtcagacgcc cgttgccatc aaatgcaata aaggattttg cgtacatcgt
     2941 catattcctc agccgccaga cgacacgcag gcaacagctg tcccggtacg ggcagtgtgg
     3001 tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa aacaggctgg
     3061 ttgctgttac cgcactgtgg ttgtcacaca atggcccacc tgcgtaaaaa actgtcagca
     3121 ggcagggatg gacatcgggg gatgtgctgt atcggttgcc ggaaggaatg gtaaagaatg
     3181 tgccatggtt aaatctccgt atggttcata cggaatagcc atgtcgtaaa aaatgcgcag
     3241 agtccctgaa gtggcctccg gaaccgactg taacaattag cgccagcccg tagttgtcgt
     3301 tcaggggctt acaccagaga acatatctcc gacagggtac tgtccagtcg cggtcggccc
     3361 cggttacttt tttcgggaag cctgctacgc cccagaataa taccgtgttc tttctgatcc
     3421 tgtttagcca ggtagctgag ggcgtaacgt aagtcgtcta ccgcagattt atcactatag
     3481 tgaatcacgt gatcgatacg caccgggtat ttgtctttag cccggcacag atgaaaataa
     3541 ccctcccctt ccgtgatccg cctccagata tctcccagtt gcctggatat ctgataagag
     3601 ttcttgtgac gttgtccatc cagatagccg ataaaatgaa tatgaagccc tttgtctggt
     3661 gtatattcca ttacccagta atatcctgct aacatcgtct gggtttcgct aagtaaacgg
     3721 tatatttcca tacacatact gtgcttacag gaatgcccga aactgggcgt gtctttcctg
     3781 taggcaaaat caattctgaa aggtaacagt ttagaaaaac gttgaaacat accgtccatg
     3841 tgttcattca catctttcag aatcgcgaaa ttcatttcgt aattgggatt ggcattatac
     3901 attttatagt ccttacttta taaaactcac aggaagagga aattatgtaa atgctgaggg
     3961 tacagaaacg tcgtactacg gcattaataa aagagataac cagcagcaca gacaacctga
     4021 taaaactgtt ttgtattacc tcattacagc aatgaggtaa tatttcagaa tacagtgaga
     4081 ctctcttaaa ctgaggacca gtacaaatta caatggtaaa aacaccacct catagaattg
     4141 agtttaagaa aacaccattc actcaacctg acattcacaa aagtcagtac caggtagcct
     4201 cagataacat cgctattaat attatcctgc cattaagtaa aaacagaaac tctgtgagga
     4261 agcctcacca atatttgacc agtagcataa agaataacga tatgagagat acgcccacac
     4321 cagagatgat tagcccatac aaaaagataa tatttattaa taccaccgaa cctcaataga
     4381 gtaataacac actaatagcg ttttagtaca cacttaacac taactatgca ccagacacat
     4441 actcaggatt aacattctca acatttatgg tactctcaaa gagtataatt caccacacag
     4501 gtaaactctc acaccagtag tatggagata atgctgtaca catacttaca gagtaaagtg
     4561 tacagctagc atacagtggc gagcagacat tacaacataa agactcaaga tagcataata
     4621 aagcataata agaacacaac aaaactccca ctaataatac taattaacct taattctttt
     4681 ttgaacagga aatcaacata gctccagacc tcttcaggtc atacactttt gtattggatg
     4741 ttcaatgtgt acttctgcac agacaaaaaa ataaaaccca caaaataaaa tatcaatccc
     4801 aataaaaaat ttt
//
LOCUS       DAZGBC010000259         4764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGBC010000259 DAZGBC010000000
VERSION     DAZGBC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.261"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHK1J_004812"
     CDS             complement(<1..275)
                     /locus_tag="ACHK1J_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7913967.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA"
     gene            complement(275..604)
                     /locus_tag="ACHK1J_004813"
     CDS             complement(275..604)
                     /locus_tag="ACHK1J_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7913968.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(601..3246)
                     /locus_tag="ACHK1J_004814"
     CDS             complement(601..3246)
                     /locus_tag="ACHK1J_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7913969.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKDVPGVSALFGELAEENREFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISQQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHEKETLEY
                     KRQLAELGDKVEYQKRLNELAQQAARFEEQQSAKQAAISAKARGLTDRQAQRESEEQR
                     LRDVYGDNPQALAQVTGALKQTWADEDMLRGDWLAGLKSGWGEWTESATDSFSQVKSA
                     ATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAF
                     GGGASASTGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGN
                     LYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYD
                     MARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(3296..3598)
                     /locus_tag="ACHK1J_004815"
                     /pseudo
     CDS             complement(3296..3598)
                     /locus_tag="ACHK1J_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(3625..4047)
                     /locus_tag="ACHK1J_004816"
     CDS             complement(3625..4047)
                     /locus_tag="ACHK1J_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7913970.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(4063..>4764)
                     /locus_tag="ACHK1J_004817"
     CDS             complement(4063..>4764)
                     /locus_tag="ACHK1J_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7913971.1"
                     /translation="LWVYTGKGDAYANPLSDDEWTRLAKIKDLTPGEMTAESYDDNYL
                     DDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDSSETRAYKIRFPNGTVD
                     VFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVAKG
                     QNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGNGE
                     FAAVAEITVTDGAAG"
BASE COUNT          926 a         1594 c         1123 g         1121 t
ORIGIN      
        1 gccatcccgg tgaccagccc gaaaagattg gacactgtca gcgacgggcg ggcactgctg
       61 ccctttccgt tcatctcaaa gccgctgccc tcaatcgggt acgcctgata ttcacgcccc
      121 tgccaggtca ctggctcccc tttttcattc agctcattgc agaaaaaata ccgctcaccg
      181 ccctgcaccg tcaggtcgat ttcccagagc accacccgcg gtgactgctc tgatttaacc
      241 gactcgttca ggctttcttc gtgaatatcc tgcatcagtt caccacctgc ttaaactccg
      301 cgctgaactc aacgcgcaac atcccgaccc gcgcagacca cccggcacag gtcaccttta
      361 tctgccggta tgcatagggt ggcttccaca aaaatgcctt ccagcccccg tgctctgcca
      421 ggaacgcttc cagatgccgg gcctcctccc gggtcacgga aagcgtcacc ctgtatgttt
      481 tcaggtcagc attcagccct gccgccatac gctgtgagta cccgtcacca aaacgcactt
      541 cacgcaccga cggctgcgag ttcacctcca tatccggctt cactttccag cgaaaggttt
      601 tcatcgcccg ctccctgata acataccgcc atcacgcaac tgcagccgga gctcatcctg
      661 tgccccttta cgggccatgt catacaccgc cttcatcagc tgcggccccg cctgtccgtt
      721 gataccgtcg ttctgaatca ccacgtgatt gttctgatta aaattaatac cttccgcccg
      781 ccgcatctgc gccggacttc cggcaccgcc gacataacca ccttccgcat agccgcgcat
      841 caggcggtaa agattcccca cacctatccg gctggttgcc tctttcgtga aaacaaactc
      901 cccgcggtga actatccccg caggctcata tttgccgccc gtccccgtaa atccgccggt
      961 cgcaaaatga aagttcgccg ccgcagcctc aatggccgtt cccgtggaag cggatgcgcc
     1021 accaccgaaa gcaccaccaa tggcgctgcc gatacgcccg acaattccca ccatggcctg
     1081 tttaagcagg atttctgtca tcatggacag caccgaacgg gtgaatcccc gccagtctgc
     1141 ctctgcaccg gtcagcatcg ccgccatatt ctgtgcaata ccgtcaaagg tctgcgtggc
     1201 agcactttta acctgcgaaa aactgtccgt cgcactttcc gtccactccc cccagccgga
     1261 cttcagcccg gccagccagt caccgcgcag catgtcttca tccgcccatg tctgtttcag
     1321 tgccccggtg acctgtgcca gcgcctgcgg attatcgcca tacacgtcac gaagacgctg
     1381 ctcttcagac tcccgctgcg cctgacggtc agtgagacca cgggcttttg cgctgatggc
     1441 ggcctgcttc gcgctctgct gctcttcaaa ccgtgccgcc tgctgtgcca gctcattcag
     1501 gcgtttctgg tattcaacct tgtcgcccag ctcagccagc tggcgtttgt actccagcgt
     1561 ctctttctca tgggccagca gggatttttc ctgcccggat aactgccgtt tcgtggcggc
     1621 ctctttcagg accgcatact gactttccgc tttccataaa tcacggcgct gctggctgat
     1681 tttctcattc gcaccgctgt gcttctccag cgtcctgagc tcagcttcaa gggcaagcag
     1741 agcctctctt gcctgctcct cttccctctc cccggcagag cgcgttttcg gtgatgtatg
     1801 ctttttacct gtcagctctt cagccagacg gctgacggct tcctgctgcc ccggaccttt
     1861 gctgacgcct gttgcacgcg agcggttgat gtaccccatt tccccctggc gtatacgcgc
     1921 atcccgttcc gcaatggatt ttctcagcgc cagttcatcg cgttttgttt tctcaataaa
     1981 ttcccggttc tcttctgcca gctcgccaaa cagcgcactg acgccgggga catcttttgt
     2041 cgtttcccag gctgactgaa taaattcagc cagcgccaga tccccctgca caagcagcag
     2101 cttcacctgt tcaacggttc ctgccaccac gtcggtgatc agactgaggg cccccagagt
     2161 atgatcacct atccatgccc atgcgtcaga agtccaggtt ttaacatcgt cccagatttt
     2221 ttccaccggc gtggccgctt tatcaagttg ctccagacgt gcattcatga catccgcaaa
     2281 cagggacatc gcctccgtta ccgcagcctg tttacctttc gtgcgctcaa gctcatcaat
     2341 atggcgtaac tgggaaacgc tcaggaagtt atactgctga ttcagggagg ccagcgcctt
     2401 caccggatct gctgcaatcc cttcaaaggc tttttccacc ttcccggcat cgtcccccac
     2461 cgtctgcagc catctctgag aggtttcccc catgatccgt agctgcccgg cggtatattt
     2521 cccgctttct gccagacggg ccagattttc tgccgcctgt ctgataccac caccggcttc
     2581 atcgctgatc accccggcca ttttccacaa ttctgccgtt gtggtggcag ccgcccctcc
     2641 ggtcagggtc agtgaacgca acaaggcccg gtcagcctgc tctgcctgcc aggcggcggc
     2701 agcaagcgcg gccagtacgg caaccccgcc acctgccgcc acacgggcca ccgacataaa
     2761 tcgtcccagt tcaccggcat tccgggcatt ttcagccagt gcatttgccg tatctgacag
     2821 cgaatcctct gatgattcag aggcatccct gatcccgaga agttcctcct tcagcagggt
     2881 aagcaggctg agcggtccac cgaatgaatc gctgatctgc cccccctgct gcagcatgat
     2941 aaggaaggga ttctgaccac cggcaagctg agtgacaata tccgtgaact gtgcgggcag
     3001 tgtgcgcatg gcagccttat actgtccgac tgatatcccg gctttttgtg cagccagcgc
     3061 ctgtcggctc agactctgct cagcagcatc agcctgtttt ctgaaagact gactgacttt
     3121 cccggacatc aaatcagcaa gcgcactggt ttcacccagc tcttttttta cccgcgctgc
     3181 ctcttcagaa aaacgggcag aatccagtgt cagcacggct gtcagatcgg caaaattacc
     3241 tgccatcgtg gtcacctcct gaaatgtcct ctgatgccat caataactgt cacaacctcc
     3301 ttccctcctc cccgaaacgg actccaccgg cgaggcccgc cgccttctgc atcagtacat
     3361 cattttcgtc cggcgtctcc gcctgccctt cctctgcctc cggagcgaac aggctgaaat
     3421 ccgccggatg catatccgga tcgcaaaaaa acaggctgag tacggcgtac gtcagcccgg
     3481 aaaaatgcat atccagctgg gtatcctgaa aataatgcgt gcagtaaaaa cgtcgccagt
     3541 cggcatattc ggtggatgtc atcccggcaa gcatggcgcg ccagtcggct ctccccatct
     3601 cacgcgccag tttcaggaca aagttcagct cgccttcgaa tgcttttttg atgttaccgg
     3661 ctcagtcgct tctgctttcc cggttggttc aggatcggca tcgtgccggt tatccagcat
     3721 acctgaaaga taaagcaccc ggttcgttgc ctgattcagt gcatcagcag gccatcccag
     3781 catcacttca cggcggatct gctgcatctc tgtctccgga gaggccagag tgcctttgag
     3841 agaatgggaa tgccatagcg acatcgccac aagcagggat gccgtttcca gatatcgctg
     3901 gttaatgtgc acggcatcat gctccgttgt ctcctgttgt gctgcgtctg aaacaaactt
     3961 catataatca aaccgctgca gcgcagacag ctccgacagc gtgacggaca ccccgttata
     4021 ttcaaattgt tctgttttca gaaacatgct ttatctcccc cctcagcccg cagcgccatc
     4081 cgtgacggtg atctccgcca ccgccgcaaa ctcaccattg ccggtgacaa cagggatctg
     4141 cgctttacct gccgcaacac ctttcacggt gatcgtgttc cccttcacgg taatggtggc
     4201 gaaattctga ttcgctgagg ttgcgcggaa cgttttatcc gttgcgcctt ccggctgaac
     4261 ggccacggtc agggtgatat tctgaccttt tgccacattc cccgttagtg gcgtcacggt
     4321 aataccggtg accggtgtga tttctccccg atcctcagcc agcgacggac gaccaatatt
     4381 ggtgattttc acagtgcggg tgatcacttc tttggcggtc acggatttac caatggcgct
     4441 cacccagccc cggaacacat ccaccgtgcc gttcgggaag cggattttgt aggcccgggt
     4501 ctcgctgctg tcaaaccagg caatcaaatc acgctgccct ttctcgccgg gcttccaggc
     4561 cagcgtaaaa ctggtgtcac cggcggattt ctgcccctgc ccggtggata cccagtcggc
     4621 atcctcatca tccagatagt tatcgtcgta ggattccgcc gtcatctcgc cgggggtcag
     4681 atcctttatt tttgccagac gcgtccactc atcatctgac agcgggtttg cataagcatc
     4741 gcccttaccg gtgtaaaccc agag
//
LOCUS       DAZGBC010000260         4646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGBC010000260 DAZGBC010000000
VERSION     DAZGBC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.262"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..996
                     /gene="traW"
                     /locus_tag="ACHK1J_004818"
     CDS             <1..996
                     /gene="traW"
                     /locus_tag="ACHK1J_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO7913972.1"
                     /translation="LASVIAQDGQATRQQMIFSNETRRLEEARKSFSVPDSICSESAS
                     GVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDSAGIHAGYCTEAE
                     YARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQIDAATAYMKNTAR
                     PSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADSTPNEATRGALTEA
                     LQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQDLQGMEGDNLLRE
                     LLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELSQAMSQGSVR"
     gene            993..1562
                     /gene="traX"
                     /locus_tag="ACHK1J_004819"
     CDS             993..1562
                     /gene="traX"
                     /locus_tag="ACHK1J_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO7913973.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            1635..3794
                     /locus_tag="ACHK1J_004820"
     CDS             1635..3794
                     /locus_tag="ACHK1J_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO7913974.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVRGAYQ
                     AQLQNSPYTPALGTTTSSTEYRTAEAAEPKSIIMKVWSGENFADKIATSLSGTGTSSD
                     QLNPLIKMKNIGDYTMGITESIWAGYTTVRVLSTAGSNSVFGRLVNTVTGLPDAAKAF
                     MEAVAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTSQDRGSRAAYGYIYLIDSMIRPLVMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLLIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMAS
                     SVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            3882..4529
                     /gene="excA"
                     /locus_tag="ACHK1J_004821"
     CDS             3882..4529
                     /gene="excA"
                     /locus_tag="ACHK1J_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO7913975.1"
                     /translation="MNKTKSRYPSWWAYYYLVRNAYFIFGIPCLLFYAFFGTLIVHDS
                     ALSQTMDFVFAYGCWALIILPLLWLYLRTRAKKKKVLQVVQQIKESSLFAPTSDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
BASE COUNT         1122 a         1237 c         1241 g         1046 t
ORIGIN      
        1 ctggcctctg tcattgcgca ggatgggcag gccacccgtc agcagatgat attcagtaat
       61 gaaacccgcc ggcttgagga agcccggaag agcttcagcg tgccggactc catctgcagt
      121 gaatccgcct ccggagttgc agctgaaagt cgccgtgcag cagcgtctgc ggctgcacgt
      181 ctgagtcagg gaggaggcgt cagcagtaaa cccatccgtg aacgtctctc ccgggcagca
      241 gactcaccaa cccgcgaggc atatgacagt gcaggcattc atgccggtta ctgcacggaa
      301 gctgaatatg cccgctttgg tggaaccgat gtctgccctg ccgtgggcga gctgccgggt
      361 ggagacagcc aggtgcgatc gctctatcag ggtgcaggca cagctgatac ccctgccgcc
      421 ctcacctggg atcagaaaca gattgatgct gccacggctt acatgaaaaa caccgccaga
      481 ccgtcagcgg gtcgtgcccc ggggaaaggt gaagtgggaa cacagaccgg gcgcacgtat
      541 gtgggcctgc agaatgaata caacggcatc atcgatgccg cttctcatcc gcaactgtca
      601 ctgatagctg acagtacccc taatgaggcc acccgtggag cgctgactga agccctgcag
      661 tcaccatccg ctgcggcata ttttgaccgg accgcatcat cagaggcccg cacccgtggt
      721 cacatgagcc agcgggagtt tgaggccttt gaagctggca gacgctatgc aaacaccgac
      781 tggcagcagg atctgcaggg catggaaggc gataaccttc tccgtgaact gctgcgcacc
      841 acagccctgc tgaactggca gatgaacgac ctgaaagagc agatacgcca gggtaatgtt
      901 atcgccgggc agcagctggc actggcggca cgacagtatt acggacagcg tctcggtgaa
      961 ctgtcacagg ccatgagcca ggggagcgta agatgaaaga cgacaacagc accgacaaaa
     1021 aaaaaccggc cccccgtctc cgccgcatcg gtgacatcgg gataaacctg ttcgcaccac
     1081 tggcagaaac ccggtggatg ctgcgcagct gccgccatat gctgggccag cataaaacgc
     1141 gcctgaagcg cctcatgccc gaccggcaac cggagagtcc ggtctgtatg acctgggaag
     1201 aagccgtcag ggccagtggc cttacggtca acgaactgga tcggcgtctg cgacgtcgca
     1261 ggatgttctg gcggacagga ttctggctga tgatgatacc ggtacttctg ctgtgcggca
     1321 tggcccttgc tgccacagcg cttccggcca tcaccctgat acgtatactg gcctgcctgt
     1381 tactgatgag tgccattgca gcactgtgtg cctcaaaaac cctgattgtg tcataccgcc
     1441 tctggcaact gaatgaacgg cgggtcagcg aggacgaaca gggcaccttc cccgattacc
     1501 tgcaggcccg gcacggctgg aaacaggcca catggctggc cattacccac cggcagtact
     1561 gaccgtccgg tctgcaattc cttttcctca ttccggaggc tgatgccctg catcccttcg
     1621 gttccggagt tatcgtgaaa atcataccca gagcactgag tgcgggtctg gcgctggctg
     1681 ccgtacctgc acaggcattc gtcacctatc aggacattgt gagcgccgcc accaatccgg
     1741 atgacctgtc gcgccaggca ctggtcacca tttttggcga cgtggtcaca aatcccctga
     1801 cagccagcag caacaccatt atcggtaacc tgttcatggt gtttaacgca attatcgcca
     1861 ttatggggat agtctggttc ctgtttattg gaatccgcca tgttgtccgc accggccacc
     1921 agggcaatgt ctttaacaac ggacgggacg tcgtcggaat gctcagtatt gtgagcggtt
     1981 ttatgatgat tgtgcccacc gcttccggct ggagtctggc acaactggtc atgctctggg
     2041 gcgcatccat catgggcatt ggttccgcca atatcatggt tcagacagcg gcagaaaaca
     2101 tcgctgaagg ctattcaatg accgtacagc cagtacatgt ctccacccgc acggcagcac
     2161 ggggaatact ggagatggaa ctgtgcaaac acgccatgaa tacgggcctc gatgacttta
     2221 accgtaccgc acgttcatca acgccgccca tgagcgaatc ctcacggaca gagaatggca
     2281 gatataccgt caccgtctca aatggcagcg ccacctgcgg gagtgtgagt ctctccgtga
     2341 aaggaaacgg cagtaacaag cagtccgcgc tggcccattt tttcaatccc ttcattgaaa
     2401 cggaatatca gggggttctg acggcacagc gctccgccat ggataccatg ctccgtgacc
     2461 tggacagcgc ggcgcaggaa tttgtgtcag cgtaccggac ccggcgcgac acgggtaacg
     2521 gcaacctgcc ggatattgaa agccgtattc agaaggcagc caaccggtat gaagaaacgg
     2581 tgcagaaggc gttgccaaca gataacgcag aaactgagcg cagggagcag ctgaaatcct
     2641 acctgaccac ctacggctgg gttgccctgg gcgcctggta ccagaccttt gccaccgcca
     2701 accagcgact ggaagacctg gccaaccgcg ctcctactgt gagtggcgtc tcgtcactgg
     2761 gcgaagccgg ggatacggag ttcctgaagg aagtcagagg agcttaccag gcacagttgc
     2821 agaactcacc atatactcct gcgctgggaa caaccacttc atcaactgaa tacagaacag
     2881 cggaagccgc tgaacctaaa agcataataa tgaaagtatg gtcaggtgaa aattttgcag
     2941 acaaaattgc gacatcctta tctggaacag gaacatcctc ggaccaacta aatccactga
     3001 taaaaatgaa aaatatcggt gattacacta tgggtattac tgaaagcatc tgggctggtt
     3061 ataccaccgt cagggtgctt tcgactgccg gcagcaactc cgtttttggt cgtctggtca
     3121 ataccgttac tggcttacct gacgccgcaa aagcttttat ggaagcagtc gctccacctc
     3181 tctacttcct gctgtttttg ctgttctgtg ccggtttttc gttatccatt tacctgccgt
     3241 ttatcccgtt tatcttctgg atgaccggca tcggcaactg gatagtcagc gtgctgattg
     3301 gatgtgcagc cgggcccctt tgggccgcca cccatctggg aacaagtcag gaccggggaa
     3361 gccgggccgc ttacggttac atctacctga tagacagcat gatcagaccg ctggtgatgg
     3421 tattcggatt cttttttgca tcggtcgcca tcgtggccgg cggcaccatt ctgaatgcgt
     3481 tgtttggcgc ggctctggtt aatgtgcagg cggactccct tacggggtta ttcagcatag
     3541 ctggattttt gctgatttat gcccgagtct gtaccacgct ggttgcggcc gtttttgcgc
     3601 ttcaggctta tctgcctgac catgtgatta attttctggg cggacgtgac ggcgttaata
     3661 tgctggggaa tatggcatct tcggtcaaag atattattgc cggcggaaac accaatatac
     3721 gcagaacacc tggcgtccgt gaagaaaaac tgaaaaatat taatggagat gataaggacg
     3781 gaataaaaca atagagtaaa aacatcaaaa ataaataaag cagtattgcc atattcacct
     3841 gaagagatta aaatatattc aggtgaatat gggggtttat tatgaacaaa acaaaaagca
     3901 gatatccttc ctggtgggca tattattacc ttgttcgcaa tgcgtatttc atatttggca
     3961 taccatgctt attattttat gcttttttcg gaacactaat tgttcatgat tcagccttat
     4021 ctcaaacaat ggattttgtt tttgcatatg gatgctgggc attaataatt ctcccattac
     4081 tctggctata tctaagaacc agggcgaaga aaaagaaagt cctgcaggtt gttcagcaga
     4141 taaaagaaag cagtctcttt gcgccgacca gcgattacga gcaactgtca ttctcgaaga
     4201 gttgttattt cgggatcgac attaaaaacg gcaccatgct gtatgtgcgt atttatccaa
     4261 acaatgtgat ggatgtgatt ggtctggata ttcacaactt tacccgcacg gttgctgagg
     4321 acggcaagct ggaaatccac accacctatg tgagtctgcc gatgattcct cttgagatct
     4381 ccggcatctc agcaaggacg ctggccaaca ccatgcacac catggctgca cgtggatatg
     4441 agtacaagga acgtttccca cagatgatcc ggtatcgggt taaagagtgg gaaaaagttg
     4501 ccggtgttcc tgttgcagaa gtattttgag gtagctatca actgttagac gataatccgt
     4561 cagccggttt tctctgaacc ggctttttct tttcaggcag aatgcctgtc tgccgtctat
     4621 ctcaggacgc cgcatggagt ctctat
//
LOCUS       DAZGBC010000261         4625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGBC010000261 DAZGBC010000000
VERSION     DAZGBC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.263"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHK1J_004822"
     CDS             complement(<1..617)
                     /locus_tag="ACHK1J_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7913976.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(732..807)
                     /locus_tag="ACHK1J_004823"
     tRNA            complement(732..807)
                     /locus_tag="ACHK1J_004823"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(772..774),aa:Thr,seq:ggt)
     gene            complement(814..888)
                     /locus_tag="ACHK1J_004824"
     tRNA            complement(814..888)
                     /locus_tag="ACHK1J_004824"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Gly,seq:tcc)
     gene            complement(1005..1089)
                     /locus_tag="ACHK1J_004825"
     tRNA            complement(1005..1089)
                     /locus_tag="ACHK1J_004825"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Tyr,seq:gta)
     gene            complement(1098..1173)
                     /locus_tag="ACHK1J_004826"
     tRNA            complement(1098..1173)
                     /locus_tag="ACHK1J_004826"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Thr,seq:tgt)
     gene            1535..2485
                     /gene="coaA"
                     /locus_tag="ACHK1J_004827"
     CDS             1535..2485
                     /gene="coaA"
                     /locus_tag="ACHK1J_004827"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO7913977.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2514..3479)
                     /gene="birA"
                     /locus_tag="ACHK1J_004828"
     CDS             complement(2514..3479)
                     /gene="birA"
                     /locus_tag="ACHK1J_004828"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO7913978.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3476..4504)
                     /gene="murB"
                     /locus_tag="ACHK1J_004829"
     CDS             complement(3476..4504)
                     /gene="murB"
                     /locus_tag="ACHK1J_004829"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO7913979.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGRVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1168 a         1168 c         1057 g         1232 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaagaatcc aggaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcacatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcgta ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcgcgagcag caccgccgta ggttttagcc agtacggtag taattgcagc ggtcagcgtt
      541 gttttaccat ggtcaacgtg gccgatagta ccgacgttaa cgtgcggttt tgtacgttca
      601 aacttttctt tagacatcga ttgtccctct aagacacgga taaatcggtg atatcaccac
      661 atcaaccaag caacatgccc gacttgttga atgcaataaa cagaaggaaa aaacagggag
      721 gagaaaagaa gtggtgctga taggcagatt cgaactgccg acctcaccct taccaagggt
      781 gcgctctacc aactgagcta tatcagcaca tcttggagcg ggcagcggga atcgaacccg
      841 catcatcagc ttggaaggct gaggtaatag ccattatacg atgcccgcat cctggaactc
      901 ggctacctga ttttcattct gcactgaata tcgagagaag ctctctttat tcgagccggt
      961 aagcgaactt atcgtctcgg gctacgccat cgcgtggccg aaattggtgg tgggggaagg
     1021 attcgaacct tcgaagtctg tgacggcaga tttacagtct gctccctttg gccgctcggg
     1081 aaccccaccg gacttgatgg tgccgactac cggaatcgaa ctggtgacct actgattaca
     1141 agtcagttgc tctacctact gagctaagtc ggcatcaagt agcgcgcatt ctatggagac
     1201 atgcgagttc atgcaactaa aaaattgcat aatttgtttt attggtcaca ttttatgcga
     1261 cacgatgaag aaacagccga atagagggca gattagcaca ctttttcaac atcattgtgc
     1321 tcaacaatgc gctcctgcta aaccataatt ctttttatca gatggaatat ctgtcacatt
     1381 gcttttcaac gatagcttcc tgggagagat tttttcttat tattcctccc catctggtgt
     1441 taccctcctg cccattaacc cattcaacag aactgtgacg cgccatggca aatatcgctt
     1501 tgccgataga gctatgaccg ccagaaacat gcttatgagt ataaaagagc aaacgttaat
     1561 gacgccttac ctacagtttg accgcaacca gtgggcagct ctgcgtgatt ccgtacctat
     1621 gacgttatcg gaagatgaga tcgcccgtct caaaggtatt aatgaagatc tctcgttaga
     1681 agaagttgcc gagatctatt tacctctgtc acgtttgctg aacttctata taagctcgaa
     1741 tctgcgccgt caggcagttc tggaacagtt tcttggtacc aacgggcaac gcattcctta
     1801 cattatcagt attgctggta gtgtcgcggt ggggaaaagt acaaccgccc gtgtattgca
     1861 ggcactatta agccgttggc cggaacatcg tcgtgttgaa ctgatcacta cagatggctt
     1921 ccttcaccct aatcaggttc tgaaagaacg tggtctgatg aagaagaaag gcttcccgga
     1981 atcgtatgat atgcatcgcc tggtgaagtt tgtttccgat ctcaagtccg gcgtgccaaa
     2041 cgttacagcc cctgtttact cgcatcttat ttatgatgtg atcccggatg gagataaaac
     2101 tgttgttcag cctgatattt taattcttga agggttaaat gtcttacaga gcgggatgga
     2161 ttatccacac gatccacatc atgtatttgt ttctgatttt gtcgattttt cgatatatgt
     2221 tgatgctccg gaagacttac ttcagacgtg gtatatcaac cgttttctga aattccgcga
     2281 aggggctttt acagacccgg attcctattt tcataactac gcgaaattaa ctaaagaaga
     2341 agctattaag actgccatga cgttgtggaa agagatcaac tggctgaact taaagcaaaa
     2401 tattctacct actcgcgagc gcgccagttt aatcctgacg aaaagtgcta atcatgcggt
     2461 agaagaggtc agactacgca aataatttgc aggggagcta atgctcccct ttcttatttt
     2521 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc gtcctgctca
     2581 agcaataaag cgccttgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     2641 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgaaagataa
     2701 ggtgccaatc cttcttgttc gaagagttcc agcgccgcgc gtaattcacg tattagcatg
     2761 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     2821 ttaacgacac tctcttcaac acgacgcatt gccatgttga tcccggctcc aatgactatt
     2881 tgcgccgcat cgccggtttt gccagtcagc tccacaagaa tgcctgccag cttgcgatcc
     2941 tgcagataga ggtcattagg ccatttgaca cgaactttat ctgctcccag cttgcgtaat
     3001 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     3061 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccgc
     3121 ccccggcgac cacggccagc ctgctggtat tctgcaacgc aggcatcgcc cgatttaagc
     3181 tctccgatac gatcaagaag gtactgattc gtggagtcaa taactggcag cacggctaca
     3241 ctaccgccat ccagctgacc caatatctgt tcagcattaa gtaactggat gggctcaggc
     3301 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     3361 atgtgtttat taatagccgc ccggctcatt cctaacgttt cacccaactg ctcacctgag
     3421 tgaaattcgc cattcgctaa cagcgcgatt aatttcagtg gcacggtgtt atccttcatg
     3481 aaattgtctc cactgcgctc acttcacctg atgcaccaat aaagcggact tcaggctcaa
     3541 gccagacatt aaatttttca cccacttttt gtcttacatg gtgtgccagt tgcaccacat
     3601 cttcgctttt tgcattgtct tcattaatga gaactaacgc ctgttgacgg tgcaccgcag
     3661 ccccacccat ttgcatccct tttagctggc actgatcgat aagccaacct gctgccagtt
     3721 ttactgaacc atccgcctgg gggtaatttg gtgctgttgg aaattgtgac agtaatgctt
     3781 tagccgtttc agcagataca acagggtttt tgaagaaact accggcattg ccattcactt
     3841 ttggatcagg aagtttggtg gtgcgcatat gacacaccgc atcaaatact tgctgtggcg
     3901 ttactgttgt aggatccaga cgagttaagt caccatacgt tagtacaggt tgccactctt
     3961 ttggcagacg cagacctacg gctacaatgg cgaagcggtc ctggtattca tgtttaaaaa
     4021 tactgtcgcg atagccaaaa cggcactctt tggcagttaa gcgcacttgc ttgcctgtcg
     4081 ccagttcaac acaatcaaca taagcgcaaa ctcgctgtaa ttctacgcca taagcaccaa
     4141 tattctggat aggtgatgag ccgacacgac caggaattaa tgccagattt tccagaccag
     4201 gcataccttc ctgcaaagtg tattttacca ggcgatgcca gttttctccg gctcctacat
     4261 gtaaatacca cgcatcaggt tcatcatgaa tttcgatacc tttgatccgg ttgatgatca
     4321 ccgtgccgcg atagtcttcc agaaaaagta cattacttcc ttcacccaga ataagaacgg
     4381 gttgcccttc tgcggttgca tgctgccagg cattgagtag ttgttgttcg tcttcggcac
     4441 atacaatgtg ctgagcatta tgatcaatgc caaatgtgtt ccagggtttt aaggagtggt
     4501 tcatagctgc tttcctgatg caaaaacgag gctagtttac cgtatctgtg gggggatggc
     4561 ttgtagttat gacgacaggg agagtttgta gaaacgcaaa aaggcccagt cttccgaccg
     4621 ggcct
//
LOCUS       DAZGBC010000262         4599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGBC010000262 DAZGBC010000000
VERSION     DAZGBC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.264"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..810
                     /locus_tag="ACHK1J_004830"
     CDS             <1..810
                     /locus_tag="ACHK1J_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO7913980.1"
                     /translation="AAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHL
                     HEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRN
                     PPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRET
                     IDNAAHVLLLPEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSR
                     FYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1019..1984
                     /gene="lipA"
                     /locus_tag="ACHK1J_004831"
     CDS             1019..1984
                     /gene="lipA"
                     /locus_tag="ACHK1J_004831"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO7913981.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAAALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2085..2288)
                     /gene="tatE"
                     /locus_tag="ACHK1J_004832"
     CDS             complement(2085..2288)
                     /gene="tatE"
                     /locus_tag="ACHK1J_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO7913982.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2417..3205)
                     /locus_tag="ACHK1J_004833"
     CDS             complement(2417..3205)
                     /locus_tag="ACHK1J_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO7913983.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILALPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3298..3681
                     /gene="crcB"
                     /locus_tag="ACHK1J_004834"
     CDS             3298..3681
                     /gene="crcB"
                     /locus_tag="ACHK1J_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO7913984.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3735..3944)
                     /gene="cspE"
                     /locus_tag="ACHK1J_004835"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3735..3944)
                     /gene="cspE"
                     /locus_tag="ACHK1J_004835"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7913985.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4119..>4599)
                     /gene="pagP"
                     /locus_tag="ACHK1J_004836"
     CDS             complement(4119..>4599)
                     /gene="pagP"
                     /locus_tag="ACHK1J_004836"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO7913986.1"
                     /translation="DEWMTTFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRY
                     NERPWGGGFGLSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLANENFHLG
                     LGFTAGVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1184 a         1189 c         1052 g         1174 t
ORIGIN      
        1 gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctataca gaaactgcgc
       61 gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc taccgcattt
      121 gcgatgcatc tacatgagta tatcagtcag ggccttgaat ccattcttgg cgcgctggat
      181 atcgaaggaa gctatgataa gcaacgaacg ataactatcg cgaccacccc ctcggtcgga
      241 gccctggtcc ttcctgtcat ttatcgggcg attaaaactc actatccgca gcttttactg
      301 cgcaacccgc ccatcagcga cgcggaaaac caactcagtc agtttcaaac cgatctcatc
      361 atcgataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt caccgacaat
      421 atggtgttaa tttgccgcga gggaaatcca ctactctctt tagaagatga cagagagact
      481 atcgacaacg ctgcacatgt actcctgtta ccggaaggac aaaatttcag cggtctgcgg
      541 cagagaattc aagagatgtt tccggaccgg cagatcagct tcaccagcta caacattttg
      601 acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag ccgtttttat
      661 aacctgttta gccgttgctg gccgctggaa aaactgcctt ttccgtcctt aaatgaggag
      721 caaatagatt tctctatcca ctacaacaaa ttcagcctgc gtgatcccat cctgcacgga
      781 gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcgc
      841 agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc
      901 tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac
      961 aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat
     1021 gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct
     1081 tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat
     1141 gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa
     1201 aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa
     1261 ccacggcaca gcaacgttta tgatcctggg cgctatttgt acccgccgtt gtccgttctg
     1321 tgacgttgcc cacggtcgcc cggtagctcc tgatgccaat gagccagtga aactggcgca
     1381 gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgacct
     1441 gcgcgatggc ggtgcccaac actttgcgga ttgtattact gccattcggg aaaaaagccc
     1501 gcaaatcaaa attgaaactc tggtgccgga tttccgcggt cgtatggatc gtgctctgga
     1561 tattctgact gcaacgcccc cagatgtgtt caaccataac ctggaaaacg taccgcgtat
     1621 ttaccggcag gtacggcctg gcgcagatta caactggtcg ctgaagctgc tggaacgctt
     1681 taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggtc tgggtgaaac
     1741 caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgttaac
     1801 gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc
     1861 ggatgagttc gacgaaatga aagccgcagc gctggcgatg ggctttaccc atgctgcatg
     1921 cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa
     1981 gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt
     2041 ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc tttatgagag
     2101 agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc gtcatcattc
     2161 atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt acgtaacttc
     2221 ttagtcccaa acagcagaac gaccagcgcc gcaactacca gcagtttggt aatactaatc
     2281 tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta aaaaccagag
     2341 aatattaacg gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg tgaatcaagc
     2401 gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa gcgacggttg tttaagacgg
     2461 gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac ttccgccata atgagtgcag
     2521 gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg ctttgcccga
     2581 tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc agcgcacgag
     2641 cggcaagcaa cgttgaccag tgatgctctt tgagcggccc gcgaacccag gcggcaggaa
     2701 gtgccaggat ttcagctccc tgtaatgcct gtgccagcgc cagctctgga aagcgtaagt
     2761 cataacaggt catcagaccg accttcatcc cttccacctc cagtaacgga gcgatttcat
     2821 taccagcatc aacacggcgt gattcctgaa tggcaaatgc atcatagaga tgcagtttgg
     2881 cataacgggc gacaatgttt cctgcctgta gcgccaccag catattccat gcgcgccccg
     2941 gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat gttacgttta ctttctcgcc
     3001 gtaaacgtcc gaggaattcg ccttccagca gctgtgctga tttaaccgat agatctgcat
     3061 catgatcatc gcgtgccagc aatgcttccg gcaggacaaa cagcgatacg tcgttttccg
     3121 ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca gatgtaacag
     3181 caaactgtcc tgcagcaacc agcattgcca cctcctgtca gagtcgtaaa attcgccaac
     3241 gacgattatt cggcgttaca cttatcactc atacaaatca aatagcagga ttttgcagtg
     3301 ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtggc gagatggctg
     3361 ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct ggctgcaaat
     3421 ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat gacaaacatt
     3481 gatcccgtgt ggaaagtatt aatcaccacc ggattttgtg gcggtcttac aaccttctca
     3541 acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg ggcgttactg
     3601 aacgttttcg tcaaccttct ggggtctttt gccatgaccg cactggcatt ctggctgttt
     3661 tcggcctcaa ccgcacacta aagaccaaaa aaaacccgct gattaagcgg gttttgaatt
     3721 cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt ggcaccgtta
     3781 gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt agtctggatt
     3841 gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa accgaatcct
     3901 ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat tacctttaca
     3961 tgaaaaatag acacaaatgc tgtgtcggtt accagtacac caattgtggt acgctttgtc
     4021 cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgtta attattttag
     4081 ttattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa gcgcatccag
     4141 gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg aaaagtcact
     4201 gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta attccagtta
     4261 tcacgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc attcgccagc
     4321 ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt ccacgagtcc
     4381 ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg cgacaggcca
     4441 aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc gtaagcgaaa
     4501 cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg ttgccaggtt
     4561 tgtgcaatat tttctctaaa cgttgtcatc cactcatct
//
LOCUS       DAZGBC010000263         4470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGBC010000263 DAZGBC010000000
VERSION     DAZGBC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.265"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(91..327)
                     /gene="cvi"
                     /locus_tag="ACHK1J_004837"
     CDS             complement(91..327)
                     /gene="cvi"
                     /locus_tag="ACHK1J_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HFO7913987.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFREKDFLSMHTFC
                     FSALMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            919..1065
                     /locus_tag="ACHK1J_004838"
                     /pseudo
     CDS             919..1065
                     /locus_tag="ACHK1J_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(1115..1468)
                     /gene="cmi"
                     /locus_tag="ACHK1J_004839"
     CDS             complement(1115..1468)
                     /gene="cmi"
                     /locus_tag="ACHK1J_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO7913988.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQFSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
     gene            complement(1646..2428)
                     /locus_tag="ACHK1J_004840"
     CDS             complement(1646..2428)
                     /locus_tag="ACHK1J_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7913989.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVTMLKNRQA"
     gene            complement(2430..2843)
                     /locus_tag="ACHK1J_004841"
     CDS             complement(2430..2843)
                     /locus_tag="ACHK1J_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913990.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            3412..3669
                     /locus_tag="ACHK1J_004842"
     CDS             3412..3669
                     /locus_tag="ACHK1J_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913991.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            3669..4259
                     /locus_tag="ACHK1J_004843"
     CDS             3669..4259
                     /locus_tag="ACHK1J_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913992.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDSGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNREG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(4277..>4470)
                     /locus_tag="ACHK1J_004844"
     CDS             complement(4277..>4470)
                     /locus_tag="ACHK1J_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO7913993.1"
                     /translation="SMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLT
                     ASFHWWRRKVNRLPPWDDV"
BASE COUNT         1162 a         1162 c         1053 g         1093 t
ORIGIN      
        1 taatataata aaaaatactt gccacgtttt catctatatt cctgcgctaa agttatagta
       61 acgccaacac ctccagaaac agaatctaat tcatttagag tcagagttct catatgatct
      121 cctttttatc ttatcggata ttgaataata attatcacca acaaagtaac atattgcaga
      181 cattaatgca gagaagcaaa atgtatgcat ggataaaaaa tccttttctc taaaaacaca
      241 atcatatata gctaatgcaa tatatattgc ggtggcattt attataaatg caaataacaa
      301 ctctaatttt gttctttttc tatccattac tttctatccc attaccatac aaacactaac
      361 gataatgatt atcgtttaca tagtcaagag tgaagggtag gggagccctc acccccctat
      421 aaggggtccg cctggaaaac ggagtttccc acgtcaagag aattgagttg aacaagtggt
      481 tacatccatc caagccagct aactcactaa acgtaaagga tgtacgaaat gttcctaccg
      541 tccgataacc atttggctta cttccgtaca ttcccttgcg tagatgttca gaattgtacg
      601 agttcggaca ccaaaaccgc tgttgtcccg ccattctggc gattcctgaa agtcatttga
      661 ctaaaaaata ggggctattt cgaattttct ggacactcct gacgcggagt ctgtgacttc
      721 aacctgacac cttacgctct tagcgtacga ttttttccgt ttcgtgagtt gaccccttta
      781 ttttccaaat tccaaactgc cccctacatt tatacaatgt ccggcagggc atagaagggg
      841 agaaagaaaa tacctgggcg aagccgctga acatcattct ggcgatttca ataaaggcat
      901 ttgctcgcgc tctgtcatta tgatgataaa tacgatttta acgccagcac tcaccgtggc
      961 gtcatcggag agtcgctcac aaggctcggg gcgatgtgtt ctggtaaaga gtaccagata
     1021 ctgcttcctg gtgaaattca cattaaagaa agtggtaagc gataatataa gggccgggat
     1081 tccggccttt ttatgcagcc tttataacag tgtattagtc attcgcatat tcaacatatc
     1141 cctcctcaca atggtacatg ccataataca ttctcttacc ctccggacct tcagctttaa
     1201 atgggacaag aagtgttttt tcatcttcaa catctgttaa acttctttca atcttttccc
     1261 atgtaatttt cgggatttct gtaccaagaa gttgtacatc gttgtcccag cgtttcattc
     1321 tttgcaggta atcatttttt acaaatgttc tggcctgttc atcgctgaac tgatagcatg
     1381 ccggaccttt atcttcagac cagaaaaaaa cagcagcaag agcaataatc gttaaaataa
     1441 aaataaattt catgctaatt actttcataa aatctccaca acttctgtcg ttcattcgat
     1501 ataaaaaaca ttacgaatat atccgtacaa tgtgagcata ctgtgaagtg gctttaacaa
     1561 ttatgaaata atacaagaga tcacatattg catgtggtct ctttttttac agaataaatc
     1621 acaattcata attatgactt atgatttatg cctgcctgtt tttcagcatt gtgacagcat
     1681 cggactcagg catcaaaaac tgcacccggt gccgtgcagc cacatccagt gcaaacacct
     1741 tcgtgtagac ctccgttgag ctgatggact tatgccccat caggctctgc aggaccttca
     1801 gcggtatacc ggcatacagc atgtgcatcg cataggaatg gcggaacgta tgtggcgtga
     1861 ccgggacaga gaacgtcaca ccatcagcgg cagcggcggc aaccgcctcc ccaatccagg
     1921 tcctgaccgt tctgtccgtc acttcccaga tccgcgcttt ctctgtcctg cctgttcgtt
     1981 tattacgccg ttccatgggg attttcagtg tggccaccat cgtctgcagc tggctgacgt
     2041 accaggtgtc ggaaagcgga accagccggt gagtctgctg accggcgggc attcttcctg
     2101 ccgtcctggc ggctttttct gtccgctgct tcagagtggc cagctgcaca aacggatacg
     2161 gaggcgtgag cgaaaaatcc ccccgcgtca gcgccagtgc ttcattaatg cgcgcaccgg
     2221 tgttccacag tgtggccagc aacatcttgc ggcgcagatc cgggacgtaa tggagcaggg
     2281 cgctcacttc aggggccagc aggtattttg gcagttcatc atgaaccatc gacatctggc
     2341 ggagtgccag cgctgccgga taatcgatgg caacaggaag ctgagcggca tcccgggcat
     2401 ggtggatcgg tgccggcagg tgctgcatat cagtgttcac tccacgggga aacgtcgggc
     2461 aggttttcca caacacggta ctttcccgga taacggagct ggttaccgcc acgcgtccag
     2521 acggtgacaa caaaaccccc tccctggttg ttttcgggga gcaggcgatc gcactgctca
     2581 ctcagcatgt ggatcacaaa cggcgcatcg ctgccgtctt caaataccag ttccagcgca
     2641 tcgcggccat gcagcgttcc cttactgatg atgacgtact cagccccccg catctccctg
     2701 agtaaatgtt ttgccgcatc cgggacaagc agcctggctg cgccggcatt ccagctgagg
     2761 tagacatatc cggcctgcgc ctgtacagac tgccagtaat ccgttccggc gacagcctga
     2821 ccttcattct caataaaaaa catgggtgac tccttttttc ttttccgggc agggacggtc
     2881 atgcgataac gtcatccatc atcactgccc ttaccgtcac gccgtctgat aacatcatgc
     2941 cgttatcatc atcaccatca taacaagcat ccatataacc acgccgttaa attaccggag
     3001 agccgtttcc gtcagatccg actttccgga atatcacaac tttccggaag tgtaccacgc
     3061 tgaggaaata atcatatcgc tgttttccgc ccctgcgcgg cgcatcacag gtcagggaga
     3121 tgacacaaca tatagtgtca tcagcagctg aattcagcac tacatgttgt gctgacagga
     3181 ggtggcgttg tgtgggggga gaggaagatc ctgaacagcc ggaaaccgga tctgcgcggc
     3241 gggaaacggg caggcgatat gataattaag caacacacat aaattcatat cagatatgac
     3301 ttcaatcata agtaatgaaa cgatatctga tattatcata aatcgataac acaaaaatca
     3361 tatagaattt gatttttacc tgtcagaaca ttatcagatg gagcgcgcag gatggatgaa
     3421 gaaagggtgt tttcactcag ttatgaacaa ctgacccggt ttgcggaaaa acgcatccgg
     3481 gaatgcaatc tggacagcca gggcgcgatt tatctctgcg agtcagccaa agctggagcc
     3541 gtgctgatct tctggcatga actggccata aatggctatg cctcaatgaa tgccataaag
     3601 agacaggagc tcattgacgc cgactttcag cgcctcagaa acctcatctg gccggaggat
     3661 gacaggtaat gacccagagc cgccgaccgt cgccactgca gcggagagtg ctgattgtgc
     3721 tggctgccct tgatgaaaaa cggccaggcc ccgtgctgac gcgggacata gaacgggtgc
     3781 tggagcgaag cggggaagcc ccggtctacg ggccaaacct gcgcgcctcc tgccgtcgtc
     3841 tggaggattc cggctggctg cgtaccctgc gcgctccgaa cctgcaactg gcagtggagc
     3901 tgacggacgc cggccgggcc gtggcacagc ctctgctgct ggcagaacag gaccgcctcc
     3961 gggcagaaca gcgtgcggca gaagtcgtcg tactgccgct gatcccggca gcaggtctgc
     4021 ccgcagacgg cacttctgcc actgatctgg cggtgcagct gaacggcata acgtatcagg
     4081 cctgccgtgg ggattttgtg gtacgtctcg acggcagcac ctgtctgcag ttatggaaca
     4141 gagaaggcag ggtggtacgc cgggagggcg atccgctgga agtggcgcag tggctgcagg
     4201 cctgtcacga tgcaggaatg gaggttcgtg tgcaggttaa tgaaagcatc accccgtaaa
     4261 aacatgcctg cacggtttac acatcatccc atggtggcag ccggttaact ttcctgcgcc
     4321 accagtggaa agaggccgtg agcagtccga acaaaaaacc acccgtgata cttataataa
     4381 tggcctcacc tgccaccatt cctgacggcc cccagtagat aaaccacatt gcacatcccc
     4441 aggaaatgcc ccataagctc cccatcgaca
//
LOCUS       DAZGBC010000264         4400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGBC010000264 DAZGBC010000000
VERSION     DAZGBC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.266"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..300
                     /locus_tag="ACHK1J_004845"
     CDS             151..300
                     /locus_tag="ACHK1J_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913994.1"
                     /translation="MNSQILPDMLINFALINITDRKNEGINTIDGNWQADEGRRYRDN
                     VRIYF"
     gene            complement(398..829)
                     /locus_tag="ACHK1J_004846"
     CDS             complement(398..829)
                     /locus_tag="ACHK1J_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO7913995.1"
                     /translation="MVLRSIWIEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(897..3608)
                     /locus_tag="ACHK1J_004847"
     CDS             complement(897..3608)
                     /locus_tag="ACHK1J_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO7913996.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGWKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRTEPQAGPFE
                     MAAADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAVCRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVIICEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(3620..3952)
                     /gene="mobA"
                     /locus_tag="ACHK1J_004848"
     CDS             complement(3620..3952)
                     /gene="mobA"
                     /locus_tag="ACHK1J_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO7913997.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            4185..>4400
                     /locus_tag="ACHK1J_004849"
     CDS             4185..>4400
                     /locus_tag="ACHK1J_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO7913998.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKA"
BASE COUNT         1102 a         1205 c          918 g         1175 t
ORIGIN      
        1 aaaatcataa tctactgatt ttaatcagtt aagctaaatc aggatgaagg ctttgattat
       61 gaattataca taaaaacaaa tatgggcagc acaaaaaatg gtgcaagggt tccccacgtt
      121 cgtaatagat tcacttctat ggatattggt atgaattccc agatcctgcc agacatgctg
      181 attaattttg cccttattaa catcacagac agaaagaacg agggtatcaa taccattgat
      241 ggtaactggc aggccgatga aggacgccgt taccgggata atgtaagaat ctatttttga
      301 gtatgtcaac ataacctgag gcaaagtacc tcaggttcca gtgtagtaat cagccaacct
      361 gacagtcttc tgttatcccc acagatatgt cgtggagcta accgcgcatg tcaaagccgg
      421 atttttgaaa atctctataa acttccggat tgttgaaggc cgggaattta gctttatggg
      481 ctgctgactt tatcgcttcg caataggctt tatcgccatt actggttgat atttttaacg
      541 ccgtgccatc ccgggagaat tccatatgca acctgcattt tttccttttc catttttgcg
      601 gatcatcaag tttagcatta attgcatccc tgattccccg tgcctgctcc ccccattcat
      661 cctgatcatc ccaacgtcct gaactgcaac tacccagggc tgtagtttta tggcaatttg
      721 aaggatgtaa tggcgcacat cccgccacaa agctgactaa aatagccagt ataacgattt
      781 tctttacccc catatattgc tcctcaatcc atatagatct cagtaccata gctctgtcag
      841 agcaaagaaa atcttaacta atacaagtta agatttttaa tatccactcc tcatccctac
      901 attccaggtg atggtggcgt ccactcatta tcctgaactg gagcataaga tgcgccatgc
      961 cccccggata tgcttttctg ttgcctgata tattcgtggt agtactgttc actgcgctga
     1021 cgatgtaaat ccacatccgg gcggctgatt acaaagtgtc catttccggt tattccagct
     1081 gcattatgcc aggccaccat ttccgcaaat acatcattaa actgttgata tgagccttcc
     1141 ggttcaaaac ccattcctgg ctcacgatca aacaatacag gagcaatcag attcagctta
     1201 tccaccagtt catttctgtc ctgatgatgg tagaacacca cacggtcccc ataatccaca
     1261 caaactaact cgccattcct ccgatcacga aaacgcacac tgccatcttt ctgcagacgg
     1321 gcttcgagaa cgccagtgtc ttcataaagt ggagttcctc ccggttcaca gataatcacg
     1381 cagcggtcgg cattcgttgt tcgtcgcttg tctttgcgac gatcacgata atcccagcca
     1441 cgtaactgtg ataatgcagc tctgtctccc tgtaccgcct gttgctccac ccactgccgg
     1501 taggacaaag gataccactt accctccgca atcagtgaca accgctcttc cttcacactc
     1561 tccttcagcc tgatcagcgc ctgcatacgc tgcacttccg caatatggta atgcagctta
     1621 cgcaattgtg gatcccggaa ctgcacacgg atatacgcct tgcgacgacg gcagacagca
     1681 tgaatttccc gcaaccgttc gccgtaacgt aaatcgggtt tacgccagtg ttccttccat
     1741 gcaagataac gcgcacgcaa atcttctctc gcagctgcac gggcttcacg gcgttcacga
     1801 cgtagcacag gatcgcgccg gaatcctggc tccacttcat cgctggcagc aagttccgga
     1861 ttgtagcgac attcaggctt cacccgttca aatatatctg cagcagccat ttcaaatggc
     1921 cctgcctgag gctcggtccg ggataacgtc aaatccgggt ggatactgct ggccttcact
     1981 ggtgtgagct catgattaaa cgcatccaca atcacaagac catgatgctg tttctgcaac
     2041 ataagccctt tacgcgcaaa catctcatgg cacgcctgcc agctgaaatt cccgcccgcg
     2101 atcatatctt caatcaacga ctcacactga acagtcgtgt acccccacag actttcctta
     2161 tctgaaaaca gttcgcgctt tattgctccc tgtggacctg tccccatccg catacgatac
     2221 cattcgtaat cactgacagc ctgagtacca agattacgtc cccggaatcg ttctccgggt
     2281 ataacctgag taaaaatatc cttcggtaca ggctgccatc caccatccag atcctgcata
     2341 taatccagcg tcattaacgg ccacacactg tctgcacgta ctggtgtctg ttgtttatca
     2401 aatccatcat gcagtaccag atgcccgtgc tggatcccca tccataatcc agcctttgcc
     2461 agagttctgt gcacatccag gcaactttcc agctcaccat ttctcgccat ttccggtaag
     2521 cgttcggcaa aatgacggca ggcgtactgt ttcagactct ccgtttctgc cactttttcc
     2581 ggccggatat caacgtttat ggcttccgga tcataacgac cgggtgtccc cacctgactg
     2641 aaaatatctg tcggtacagg ctgatattcc cccagtttcc accccagttt ttcagctgtc
     2701 catgacggtc cgaacgaact gagtgcaaca ccttcccgcg ctctgtccca gccatccttc
     2761 accaccagtt ccccttcctg catcgtgata tacagaccat cactggccag ccgtttatgc
     2821 aaagataacc agtcctgtgc tggctcttca cgcagcccgg caatcgacat ctgagcaata
     2881 tattccctga acgtctgctt tttcccgcgt cgccaggcat tacggcgctc acgtaccagc
     2941 gctgttttgc gcacaatacg gttgccgggt gcatgcacaa agcagccatt atccggcgca
     3001 aatccgtgct taagttcaag ttcacggcat gcacgactga gtttttcctg gctccaggaa
     3061 agccagttaa tataaccagt ttcaggatgt acacgattca cggcaacatg cacatgcaga
     3121 ttatccgtgt ccgtatgaac ggcagccacg tactgatgtt ttgagagttc aagagatttc
     3181 agtgtgtgac gcacacagtc aaaaagctgc tcaggacgcg ggctttcatg cgcaggccag
     3241 gaaagaatgt agtggaaaac ggggtctgta tcattacggg aaaaaacagc tttgtccgcc
     3301 ttgtactgca tctcttcagc agctgtttcc agtgaattgc agttatggaa acaggttacg
     3361 ccatagaaat ttacccactg actgccgtct ttcatcacat caatcaggga gacaaatttt
     3421 tcgttacgca ggcgtgttgc ataatcaaca agccgtctga aacggttgcg gtgagggaca
     3481 tcactcttga tttcgctgtc ttctgtgagc tcattttcct gcacatcatc ccgcacagac
     3541 gtgtacgcaa tcaggtcttc aaaggaagac ctgccgtcac gtcttttgtg cggaataatg
     3601 gcattcatca gcagttatct cagcgtccca ttaaatctct tcgcagagca ttgacatatt
     3661 cagtaatggc aacaagaacc tctgcatact ccttatcacc agtgcgtttt ccctccacaa
     3721 acagatgctt ctgaaggcgg ccaagacgca taatctcatc aatcatacgg tcgtcagtac
     3781 ggcagttaac caccttatcc agtgaagagc ttcgtataaa tgttgaaacc gtctgtccac
     3841 ttgactccgc tttttctctt acgatttcag cctcatcctc cgtgaatctc gccgtgaatt
     3901 ttacaattct cttcctgttc tcactgcccg ttcgggtttt cttctcactc atggtctgct
     3961 ccttaccggg gtgtcggggc aaagccctga ccaggtgggg aatgtctgag tgcgcgtgcg
     4021 cggtccgaca ttcccacatc ctgtcccgtt tttcggtcca ttatagcctg ttatttttgt
     4081 gcatcatagt cttatgcaga cggcagaggt agggcacagc cctgccggga gtcagttttt
     4141 gatgataaaa tcaggcttca acatgaaacg gagagagagt gccaatggct tctaaaaaat
     4201 tttactcaga tgatgacatc cagctggcaa aagctgcttt atcggaattg ccggacctca
     4261 cagctcaacg gaaaacattg cgtgattttc ttgatgccat tcgtgatgac attattgtcc
     4321 tggtaaggac aaagggttat acactggcag atgttcgtga cacgctcaaa aatgcagggt
     4381 atgaagtagg agagaaggca
//
LOCUS       DAZGBC010000265         4394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGBC010000265 DAZGBC010000000
VERSION     DAZGBC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.267"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..197
                     /locus_tag="ACHK1J_004850"
     CDS             6..197
                     /locus_tag="ACHK1J_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7913999.1"
                     /translation="MNISKETREILHNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            160..513
                     /locus_tag="ACHK1J_004851"
     CDS             160..513
                     /locus_tag="ACHK1J_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914000.1"
                     /translation="MVTTSFRAAGTGDTGQAVEAACQPARKPLAGWRLPEGMRATLAR
                     RPLPFLLVTGVVSGSTCYRPVVKEPSYTCAPSAPGCLAASGRQARLRQAAACAPASLR
                     RTRWRGFGLPLRSDA"
     gene            complement(513..581)
                     /locus_tag="ACHK1J_004852"
                     /pseudo
     CDS             complement(513..581)
                     /locus_tag="ACHK1J_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            950..1477
                     /locus_tag="ACHK1J_004853"
     CDS             950..1477
                     /locus_tag="ACHK1J_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7914001.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGDDYGFSDDIPF"
     gene            1535..1768
                     /locus_tag="ACHK1J_004854"
     CDS             1535..1768
                     /locus_tag="ACHK1J_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO7914002.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            1827..3785
                     /locus_tag="ACHK1J_004855"
     CDS             1827..3785
                     /locus_tag="ACHK1J_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7914003.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTKFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            3840..4274
                     /gene="psiB"
                     /locus_tag="ACHK1J_004856"
     CDS             3840..4274
                     /gene="psiB"
                     /locus_tag="ACHK1J_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7914004.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            4271..>4394
                     /locus_tag="ACHK1J_004857"
     CDS             4271..>4394
                     /locus_tag="ACHK1J_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7914005.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYA"
BASE COUNT          968 a         1212 c         1371 g          843 t
ORIGIN      
        1 gggtaatgaa tatcagcaag gaaacccgcg aaattctgca taattacagg gccgtgatta
       61 atgcgcggcg tcgtgaaatg gggcagaaac cgctcaccac cgcgcagatt gttgatgaaa
      121 tctgcgattt tgtggcgaat cagcaggcgg ttttcctcgg tggtcactac atccttcagg
      181 gcagcaggca caggtgatac agggcaggcg gtggaggccg cctgtcagcc cgcgcgaaaa
      241 cccctggcag gctggcgcct gccggaaggg atgcgggcca cgctggcgcg tcgcccgtta
      301 cccttcctgt tggtgacagg cgttgtttca ggaagtacct gttacagacc tgtcgttaaa
      361 gaaccttctt acacatgcgc cccttcggct cccggctgcc tggcggcatc cggtcgtcag
      421 gcccgactcc gacaggcagc tgcttgcgca cccgccagtc tccgccgaac ccgctggcgc
      481 ggtttcgggc tgccgctacg gtccgatgcg tgaaaacccg ggcttgcagc gaccgttccg
      541 gtcgcgccgc ccctgtcgcg ccgtgacctg cgctccctgc acacctgacg gtgtgtgagg
      601 aaactgcgcc tgatggcgca gcagtcttcc ctcctgcagc cacccctgcg gcctgtttcc
      661 cgctgtggac tttccggggg gcagacgtaa ccccctcccg ttccatctgt ccagggtgag
      721 atgcaccggc cacaaaattt ttagccatga acccggctaa aaattttgcg tccgcccccg
      781 gacagctgtc ccgtgaggcg gtaaacgggc ccccatcgga aaggtccact gcggggagaa
      841 acccctgtga agtggacggc aggaggagct ttcgctcctc tgaaggggca catgaacgtc
      901 ccgtcgccgg tatccggttt atctcacatc tttcagtcag gaggttctta tgagtgcacg
      961 tggtatcaac aaggtcatcc tcgtcgggcg tctgggcaat gatccggaag tccgttacat
     1021 ccccaacggg ggcgcagtgg caaacctgca ggtggccaca tcagaaagct ggcgtgacaa
     1081 acagacgggg gagatgcggg agcagacgga atggcaccgc gtggtgctgt tcggcaagct
     1141 cgcggaagtg gcaggtgaat atctgcgcaa gggtgcgcag gtctacatcg aaggtcagct
     1201 tcgcacccgt agctgggaag ataacggtat cacccgttac gtcactgaaa ttcttgttaa
     1261 gaccacgggc accatgcaga tgctggggag tgcaccacag cagaacgctc aggcgcaacc
     1321 gaagcctcag cagaatgggc agccacagag tgctgacgcg acgaaaaaag gtggcgcgaa
     1381 aacgaaaggc cgtggacgta aggccgcgca gcccgagcct cagccgcaac cgccggaggg
     1441 tgacgattac gggttttcag acgatatccc gttctgactg gcctgactgt gataaccgcc
     1501 ccgccctgcg cggggcatca ccggagatat gaggatgagc gaatatttca gaatacttca
     1561 ggggctgcct gacggctcct ttacccgcga acaggcggaa gccgttgccg cacagtaccg
     1621 gaacgtcttt atcgaggatg atcagggaac gcattttcgc ctggttgtcc gtcaggatgg
     1681 cacgttgatc tggcgctcct ggaattttga ggacggtgcc gggtactgga tgaaccagta
     1741 catcagggat ttcgggattc ttaagtaaga gaggtgccgg acgcgcgaac gtccggcagg
     1801 acataagcaa ttataagggg atgattatgc ctgtaacgaa gtgtgaacca gaaaccaccc
     1861 gcaaagcaag ccgtaaatct gtaaaaacgc aggaaactgt cctgtctgcc ctgctggcgc
     1921 agacggagga agtgagcgtg ccgctggcct cgctgattaa atcaccgctg aatgtgcgca
     1981 cggtgccgta ttctgcggag tccgtcagcg aactggcgga gtccattaag ggcgtcggac
     2041 tgctgcagaa tctggtcgtg cataccctgc ctggtgaccg ttacggtgtc gccgcaggtg
     2101 gtcgccgact ggctgcactc aacatgctgg cagagcgtgg catcattccg gctgactggc
     2161 ctgtacgcgt gaaggttatt ccgcaggagc tggcgacggc tgcatcaatg actgaaaacg
     2221 gtcatcgtcg ggatatgcac cctgccgaac agattgccgg attccgtgcg atggcgcagg
     2281 aaggcaaaac gcctgcacaa atcggtgatt tgctgggcta ttcgccccgc cacgttcagc
     2341 gaatgctgaa actggcagac cttgcgcctg tcatcctcga tgcgctggca gaagaccgca
     2401 tcaccacaga gcactgtcag gcgctggcgc tggagaacga caccgcgcgt caggtgcagg
     2461 tgtttgaagc cgcctgccag tcgggatggg gcggtaaacc ggaagtacag accattcgtc
     2521 gtctggtgac cgaaagtgaa gtggcggtgg caggtaacag taaattccgc ttcgtggggg
     2581 ctgatgcttt ctcacctgac gaactgcgta ctgatttgtt cagcgatgac ggggacggtt
     2641 atgtcgaccg cgtggcgctc gatgctgccc tgctggaaaa actccaggct gtcgctgaac
     2701 accttcggga agccgaaggc tgggaatggt gcgccggacg catggagcct gtcggtgagt
     2761 gccgtgagga tgccggaaca taccgctgtc tgccggagcc ggaagcggtg ctgacggagg
     2821 cagaagaaga acgcctgaac gaactgatga cgcgttacga cgcgctggaa aaccagtgtg
     2881 aggaatccga cctgctggaa gcagaaatga agctgattga ctgcatggcg aaggtcaggg
     2941 cgtggacgcc ggagatgcgt gccggaagtg gcgtggtggt gtcctggcgt tatggaaacg
     3001 tatgtgtcca gcgtggtgtg cagttgcgca gtgaagatga cgcgactgat gacgctgacc
     3061 gcacggaaca ggtgcaggag aaagcgtcag tggaggaaat cagtctgccg ttgctgacga
     3121 aaatgtcttc cgaacgcacg ctggcagtcc aggcagcact gatgcagcaa ccggacaaat
     3181 ctctggcact gctggcatgg acgctctgcc tgaatgtgtt tgacagcgga gcgtacagta
     3241 aaccagcaca aatcagcctg gaatgtaaac attattcgct gaccagcgat gcgccgtcag
     3301 ggaaggaagg tgccgcattc ctggcgctga tggcagaaaa agcccgtctt gcggccctgt
     3361 taccggaggg atggtcacgg gacatgacga agttcctgtc actcagtcag gaggtgctgt
     3421 tatccctgct cagtttctgc accgcatgca gccttaacgg tgtccagacc cgcgagtgtg
     3481 gtcgcacgtc acgcagtccg cttgactcac tggagagcgc catcggtttt cacatgcgcg
     3541 actggtggca gccgacaaaa gcaaacttct tcggacacct gaaaaagccg cagattatcg
     3601 cagccctgaa tgatgccgga ctgtccggtg ccgcacggga cgcagagaag atgaagaaag
     3661 gcgatgccgc tgaacatgca gagttccata tgaaagacaa ccgctgggtg cctggctgga
     3721 tgtgttcacc acgtccacag acggatgcca ctgaacgcgc cgataacctg gctgatgccg
     3781 cctgatgaac aaccacaccg ccccgccgga gacggggcgg cagagggaga aatacgatga
     3841 tgaataccga actgacactg aatgccctgc agtccatgaa tgcccaggaa tatgaagaaa
     3901 tccgtgctgc gggaagcgat atgcgccgta atctcactca cgaggtgatg cgtaaagtgg
     3961 acgcaccggc taactggatg atgaacggag agtatggcag tgagttcggg ggctttttcc
     4021 ccgtccaggt tcgtttcacg ccagcccacg aacgtttcca cctggcatta tgttcgccgg
     4081 gagacgtctc tcagctctgg atgctggttc tggtgaatgg tggtggacag cctttcgccg
     4141 tcgttcaggt gcaacatatc ttcacgcctg ccgcgatcag tcacacgctg gcgcttgccg
     4201 cgacactgga tgcgcaggga tacagtgtta acgacatcat ccatatcctg atggcagaag
     4261 gaggtcaggc atgagcgcac gttcacgggc actgatcccc ctcagcgcag aacaacaggc
     4321 cgccatgcag gcggtggctg tcacagaaca acgtcgtcgt cagggacgca cactttcagc
     4381 atggccttat gcca
//
LOCUS       DAZGBC010000266         4367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGBC010000266 DAZGBC010000000
VERSION     DAZGBC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.268"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(39..254)
                     /locus_tag="ACHK1J_004858"
     CDS             complement(39..254)
                     /locus_tag="ACHK1J_004858"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7914006.1"
                     /translation="MPVMEQKTLSAEPRRSFSNEFKLQMVKLALQPRVFVARIAREHD
                     INDNLLFKWLRLWLIKGKRKPTAVCSH"
     gene            complement(376..3057)
                     /locus_tag="ACHK1J_004859"
     CDS             complement(376..3057)
                     /locus_tag="ACHK1J_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO7914007.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDGEVMLTCHFRSGKKYTRP
                     LSVCQITPTVILRNVFLERKGNAVTSRAERVIIYGDKYAAVYYRESERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVADERVFYARGNNKNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLMAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQRLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANMIDDVLQQVKKHLNAINQVASLKAEINELSVEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKSIREKQISLNEYEKVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMSFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQQSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIRYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSDDSRFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHRELLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLSG"
     gene            complement(3731..>4367)
                     /locus_tag="ACHK1J_004860"
     CDS             complement(3731..>4367)
                     /locus_tag="ACHK1J_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO7914008.1"
                     /translation="TVSVVIPGWHFLTQPSPAEQQLRALLAPLPDVLSADQMAQLSRT
                     PSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPGNPHVQEMVDNWH
                     KSVRSRALPEEAMTGWNEGMARLQQLAERLNRLDEQRGKYMTVSELKTEVFGIMQAFN
                     RHIPAEEQLRRYGEARNQNGSEQQQKQAELALNQLINRYQMEHTGNQERQP"
BASE COUNT         1184 a         1051 c          858 g         1274 t
ORIGIN      
        1 agtttgaaaa ggtgggcacc tacgttacca atactggctt aatggctaca tacggcagtc
       61 ggtttacgct tacctttgat cagccagagc ctgagccatt tgaacagaag attatcattg
      121 atatcatgct cccgggcaat acgggcaaca aaaacccttg gttgtaaagc cagtttaacc
      181 atttgaagtt taaactcatt tgaaaatgac ctgcggggtt ctgcggataa tgttttttgt
      241 tccataacag gcatccacta gtttgaaaag gtgggcacct acgttaccaa tactggctta
      301 atggctacat acgacggtcg gtttacgctt accagaaaac taatagtgct atatgcaatt
      361 tttaatattc agactttagc ctgacaggcc tgacaaaaac gcgcccaact tcccttctat
      421 atcactgtct atagtacgta atcttagcaa tggcagacca catttcttca gtatactgtt
      481 tttaagttca tcacgttcag cctggacact ggtcagatga tagcctccat ccacttcaat
      541 aacccctaat ggagtcttcc cgaccttata gtaaacaaca aagtcacaac tggcccgatt
      601 acgcatgaat aacagttcac ggtgagtaaa atctccccgc tccagcaaca ccaactgatt
      661 cagcgctatt tgtgaatgaa acatcatact ccgataacta tcctgcgaca ggatatcccg
      721 caacaggcag gccacaatct gttctgactt aaagcgtgaa tcatctgaat tcaatctact
      781 attcagtcgt tccagtgatt tgtcatactc actgtacagc aaatcaaatg ctgatatcac
      841 cgggctctcg aaaatctctc cgtcatcagc gtagtatctg atgtaacgaa tcagtgccgc
      901 aatatgtcca gcgtggcgtt caaatacatc attaccagtc accaacgtaa atttgtttcg
      961 ggcccttgaa acagcgacat tcaccagttc aggattatca acaaacgcga tattccgact
     1021 ttgctggctg ctgcgtttct tatccagcac agtggaaaac acaatttcat cgcactcccg
     1081 tccctgaaat ttatgcaccg tcgatttcac aaaatcagcg ggcagcactg tttcagccag
     1141 attaacctgt gcattatatg gggcgatata tcccctgcta ctttcctcat cccagtaatg
     1201 tccttccaga gattcaagct ccctgagatt tgaaaaatta cgggtatggt ttccttttgc
     1261 cgtgatgatc agagataatg aggcttcacc tgaatcaagc gttagcggta tcagagcatt
     1321 atcataaaac tgcttattac aaaattgaat gattttagga tgacagcgat aatgctcttt
     1381 aagaagagtt actggcacca tattcctgaa cgacatacaa accgaatcaa gtagactata
     1441 tttctcacaa ttatagtatt ctgccggtgg agatgggctg ttcgggagta atacaggaac
     1501 atgtggaagt tgctttcgat cccctacaac gattacatta cgggcacatc ctagtcccag
     1561 aatgcccgga acaatatcct gctgtgaggc ttcatcaata atcacataat caagaagagc
     1621 ccccttaccg atagagttaa taatagaatg cgtgctgcta ccaataaccg gaaaacgctt
     1681 tataaaacgg ttaaattcat ccctgtatgt ctctgccgag aatgaatttc ttgttgaaat
     1741 atttcttcgc agatgttgtt ttaaataaag cacagaccat gatgtcaggc gctccagtaa
     1801 aattttaaaa tcagattcct ttaatacttt ctcatattca ttcaaagaaa tttgtttttc
     1861 ccggatggat ttttcataat aagaaagctg aagagaagta aatagtgcca gccttctctc
     1921 cggaatcacc aatggtttga ctttcagtat cctgaagttt aacaacaaat caattctgtt
     1981 tctgaatcca atccgcctgt cagaaaggta atgaatataa gccatcaggt cagttgtttt
     2041 tcgtgaggaa agtcggtatt tgtgagaaaa aagctcctcc ggtcgaagat tctgactctg
     2101 ccattgctgc aggtatttat actcaacact cagttcatta atttctgctt ttaacgaagc
     2161 aacctggttg atagcattca ggtgtttttt cacttgttga agtacatcat caatcatatt
     2221 tgcagacggt gagtcaggta agacttcctc agaacgcgaa atcgacgttg aaaaaaactg
     2281 ttgtctgttc tccgtacttc ccagtcgtgc caccacatat cccagtcgtt gtttgtccat
     2341 tttttcataa acattactga cagctgagtt attattggac aaaattgcca ctgttttatt
     2401 ctgaattaat atattggcaa caatattgag aatagtctgt gttttacctg tcccaggtgg
     2461 tccttcaata actgaaatct gtgaggaaaa tgcccgttca accgccatta actgactctc
     2521 attcaggcca aacggaaaaa tcaaccccca tggcgaatca cgttttttac tctgtcctga
     2581 acagtatgca tgcaaggcgg tatccggatg aggaactatt ttctttatct gtctgaggat
     2641 attctcatcg atatttttat tattacctcg agcataaaaa acacgttcgt cagccaccct
     2701 gcacaagtaa ttaaacacgg catgctctgt aaatgctgaa cactgttgaa agtcaagtcc
     2761 ggtcgttttc atgatgtagg gtcgttcgct ctcacggtaa taaacagcag catatttatc
     2821 accatagata ataacccgtt cagcccggga tgttacagcg ttccctttcc tttcgaggaa
     2881 aacatttcgc aaaattacag ttggcgttat ctgacataca gaaagcggac gagtatattt
     2941 tttcccgctg cgaaaatgac aggtcagcat aacctctccg tcatcatttc cagcccacag
     3001 agtccagtcg gtaattttat gagtcttatt ttcaccgtca acacaaatcg tgaccatatt
     3061 ctcactcagt aattatcatt taacttacgg aaaattgtta tgaaactccc gatatttacc
     3121 cggtaaaata cttgtcattg tttgaagccc tccctaaaaa atgaccaggc atttgataac
     3181 ccggtgtatt tctcccgata tgagctccgg aggattttcc acaactccgg gtgtgttaac
     3241 atattcccgc cggccatata tacattgagt tcagtcataa cagtcaatac caagattaca
     3301 ggaacgcgat gctgatacgc tggttattga atactggtag cccctaacta ctaattcatc
     3361 acgatactct gtacttactg ttttttacaa ccattccccc aggtaaaaga gtccatttat
     3421 catgcccctc tttaacgcct gccagcataa tttaagaatg gataccgtgg gtggaattaa
     3481 caatacctgc tcaggccaat gccatgtaac cgcctcactg aaaaagccag atcatctaac
     3541 caggtacaga ccaacatcgc aaaaattacc atgtagcacg aactgctgtc atatttctgt
     3601 tcaggtatgc cgctatttcg cgggttttct tcacgccata atgcgtttca aggtagcgaa
     3661 catcttcgat tgtccatggt gtgcgcgctc ttccgggggt attttccatt tccatgtttt
     3721 atctcagcca ttatggctgc ctctcctgat tgcctgtgtg ttccatctga taacggttaa
     3781 tcaactgatt cagcgccagt tctgcctgtt tttgctgctg ttcactgcca ttctggttgc
     3841 gggcttcacc ataacgacgt aactgctctt ctgccgggat atgccggtta aaagcctgca
     3901 tgatgccaaa cacctctgtt ttcagttcac tgaccgtcat gtattttccc cgctgttcat
     3961 ccagacggtt caggcgctca gccagttgct gtaaacgggc catgccttcg ttccagcccg
     4021 tcatcgcctc ttccgggagc gctcggctcc ttacgctctt atgccagtta tccaccattt
     4081 cctgaacatg tggattgccg gggaaaagtc ccctcagttg ctgcagcagc tgcgcactgc
     4141 gttgcaggtt gtatgccggc ggtagttctg tcagtcgcgt tatctgctga ccggaaaggg
     4201 ttatccactc tgacgtatga tttaacagtg agggtgttct gctgagttgc gccatctggt
     4261 ctgctgaaag cacatccggt agtggtgcca gcagcgccct gagttgctgc tccgccggtg
     4321 atggctgtgt cagaaagtgc catcccggaa tgaccacgct gacagtc
//
LOCUS       DAZGBC010000267         4265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGBC010000267 DAZGBC010000000
VERSION     DAZGBC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.269"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(252..405)
                     /locus_tag="ACHK1J_004861"
                     /pseudo
     CDS             complement(252..405)
                     /locus_tag="ACHK1J_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312755.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(514..795)
                     /locus_tag="ACHK1J_004862"
     CDS             complement(514..795)
                     /locus_tag="ACHK1J_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7914009.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRVPASRL
                     HGHADRYKIKLRTSGYRLVYQVIDEKVVLLVISVGKREGNEVYQIADMR"
     gene            complement(785..1036)
                     /locus_tag="ACHK1J_004863"
     CDS             complement(785..1036)
                     /locus_tag="ACHK1J_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7914010.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVVILNRNEPAFYC
                     VPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
     gene            2230..2430
                     /locus_tag="ACHK1J_004864"
                     /pseudo
     CDS             2230..2430
                     /locus_tag="ACHK1J_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(2477..3583)
                     /locus_tag="ACHK1J_004865"
     CDS             complement(2477..3583)
                     /locus_tag="ACHK1J_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO7914011.1"
                     /translation="MNKTITALTVMMSSFAANASVLPETPVPFKSGTGAIDNNTVYIG
                     LGSAGSAWYKLDTQAKDKKWTTLAEFPGGSRDQATSVFIDGNLYVFGGVGKNNEGLTQ
                     VFNDVHKYNPKNNTWVKLMSHAPMGMAGHVTFVHNGKAYITGGVNQNIFNGYFEDLNQ
                     AGKDSKTTDKINANYFNKKSEDYFFNKFLLSFDPATQQWRYAGESPWYGTAGAAAVSS
                     GDKTWLINGEVKPGLRTDSVFELDFTNTGVNWSKLPSVSSPDGVAGSFAGISHDALIF
                     AGGAGFEGSRKNYQNGKNYAHEGLKKSYSADIYLWYNGKWGKSGELPQGRAYGVSLPW
                     NDSLLIIGGETTGGKAVADSVLISVMGNKVTVQN"
     gene            complement(3603..>4265)
                     /locus_tag="ACHK1J_004866"
     CDS             complement(3603..>4265)
                     /locus_tag="ACHK1J_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO7914012.1"
                     /translation="VISNAATLDIRGGYRSGSHAYETRLKVSEGWQNGWWASLESNTW
                     NTIHDNKKENLALNDVQAEINYAIKLDDKWSVRPGMLTHFSSNGTRYGPYIKLSWDVT
                     KDLNLGIRYRYDWKTYRQEDLSGKMDRDNAHRWDGYLTYKINKDFTFAWQTTVYSKQN
                     NYRYGNHKKDATENAFVLQYHMTPDITPYIEYDYLDRQGVYNGKDNLSENSYRIGVSI
                     KL"
BASE COUNT         1079 a          997 c          911 g         1278 t
ORIGIN      
        1 ctcctgagca atgtaaaaat catatatctt tttactatat aatttctggc gtatgtgctc
       61 aattcagacc ttacaccctt ccgcttcctc gcccactcgc tcactaccgt tcggtcgctg
      121 gggctgcggc gagcgtattg taagtttttc aatacagacg acgttctccc tggctgatta
      181 atattgtgta ataatacata tcgttttcag gaataatgcg gtcaattact ctgatgggca
      241 aggccagatt acctggcagc ttcggaaatc ccccctagtg cctcgattac ctggtgtaac
      301 gcaacaaaaa aaaccgccta aaccaccagg ctcatttatt tcttccaggg cattagcaag
      361 ataagcgttt gcataatcag gatctgcgcg caattcttca atcatggcga cgttgtgatc
      421 aacggttgtc gaatagcatg aaagattcaa gtgagcctcc agagaactaa ttcagggctt
      481 tcaaaaaaga atccttgctt gattatcagg atttcagcgc atatctgcga tctgataaac
      541 ttcgttacct tccctttttc caacggaaat aacgagtaaa acaacttttt catcaatgac
      601 ttgatataca agtctgtaac cagatgtacg aagtttgatt ttgtaacggt cagcatgacc
      661 atgtaacctg gatgcaggca cgcgtggatt ttccaggcgt tcagacagtt tctttttgaa
      721 ttgttcgcgg atggtgtggc ccagcttctg ccattccttc agtgccctgc ggtcaaaagc
      781 cagttcatag gtcatccagg ttaaccttca caatttccgg atttttcatg cgctcatcag
      841 caacagcgtt taattcggca tcttcagcga gttcccgata ataggcgtaa agttttgggg
      901 ggacgcagta gaacgctggt tcgtttcggt tcaggatcac aactgcgtct ccctcgcctt
      961 cagctacggt tcccatagga ttttttttca ggtcagtaat actggctgct gtagttgtca
     1021 gaatttgata gctcataagc cctccgtttg tctcgttgag ataatagcat ctttaaaaac
     1081 actcttaaaa gacctttatt ttgaaggggg gatgaaactg atggaatagg gggtccaggc
     1141 cgcaaccgcc gaaatttccg ggatgcacaa attttatgtt cagcaacctg tacgtcgaat
     1201 tatctcctta acgaacgttt tcgtacggtt ggccctcaaa cctcaatttc aagcagagcc
     1261 ctttttgatg ccgcgccagc ggcatggaag gcggcactct gtggttacct gtggcacagg
     1321 tgtaaaaccg ccgaagcccg gcgtaagccg gtacgtattg aaaggttttg cctatcccat
     1381 tcccatcctg ctaagccata cccgctttca gtcatgatga agtaagagtg cgcggtcgga
     1441 gtggtcccga cgagggttta cccgaagtcg gggcgtatct ccgcgttagc gggccgtcag
     1501 ggccgcttac gagcgcgtat tcggaacatc cagccagaag actgacagcg atgactatgc
     1561 ttagttataa cattcataat taaaagcgat gttgttcccg cccttcgggc gggggcggcc
     1621 tcgcttttca gttatgaggg gtgggcacgg tgctgcgttt gtgtggtgtt tttattcctg
     1681 atgttttttg ttgtggtttc agaccggtga ggttcggggc ccgcgcgtta gcgtgggagg
     1741 tgcttttaga ttctgagcgg cgcggccttg gccgctgcaa gcgaaggcct ctttgtattt
     1801 gttcacagtg gtttcagaga taaagacttt ccctgcggcg aagcaacgca ggggattaaa
     1861 taatgaggtt aagactatat tgcagctttg ctgtttcaag tttgcagctt cttaaagttc
     1921 tttgtttgcg gatttatatg gataactgaa acgacgtggc cacgttctga tgactgatgc
     1981 gtggttttat gggcatatag gggtcagttt ggatatagag aattattgta cggcaagcct
     2041 gttttttgaa cagacttgcc tgtcgcaata gagtgtactc ccgtgctata cggcgtttgc
     2101 agggaatttg accttaatcg gataacctaa agtgcgacga cttaaaaagc gatagccgct
     2161 gacgcaaaat caatccgtta cgcttctcga ttgattaaat aatgctctta acgtacaaaa
     2221 ttttccgata tccaaaccgc caccgagcct ggtggtttag gtagttttct tgttgcgtta
     2281 caccaggtaa ttgaggcgcg aagaagggat ttctgaagct gccaggaaat ctggccttgc
     2341 ccgccagagt atttacccgc attatccccg aatggaaccc cgaccatcac aacacttgca
     2401 caactaacat ctggaacctt atttaaatga caccaaaaca ttgataacag ggctccctaa
     2461 ccggagccct gatgagtcag ttttgtaccg taactttatt acccatcact gagatcaaaa
     2521 cggaatccgc aacagcttta cctccagtcg tttcacctcc aataatcaat aagctgtcat
     2581 tccagggtaa tgacactccg taggctcgcc cctgtggtag ttcacctgat ttaccccatt
     2641 ttccgttata ccagagataa atatcagcac tgtaggattt tttaagacct tcatgcgcat
     2701 aattcttacc attctggtaa tttttccgcg aaccttcgaa gccagctcct ccggcaaata
     2761 taagcgcatc atgacttatt cctgcaaagc tacctgcaac gccatcaggt gatgagacgg
     2821 aaggtagttt actccagttt acccctgtgt tcgtaaagtc cagttcaaac acgctatcgg
     2881 ttcgtagccc gggtttgact tcgccattaa taagccaggt tttatcaccg gaactcactg
     2941 ctgctgcacc agccgtacca taccaagggg attcaccagc gtaacgccat tgctgtgtcg
     3001 cagggtcaaa agacaacaga aacttattaa agaaataatc ttctgacttt ttattgaaat
     3061 aattagcatt aatcttatct gttgtttttg aatcttttcc ggcttggtta agatcctcaa
     3121 agtatccatt aaatatattc tggttaacgc cacctgtaat ataagcttta ccgttgtgga
     3181 caaaggtcac atgccccgcc atccccattg gcgcatgcga catcaattta acccaggtat
     3241 tgttttttgg gttatattta tggacgtcat tgaatacctg tgttaatccc tcattgtttt
     3301 taccaacgcc gccaaataca tacaggttac catcaataaa aacagaggtt gcctgatctc
     3361 gcgaaccccc cgggaactca gccaaggttg tccatttttt atctttagcc tgagtatcca
     3421 gtttgtacca tgcggaacca gcgctaccta aaccaatgta aactgtgtta ttatcaattg
     3481 caccagtacc acttttaaac ggtactggtg tttccggtag tacggacgcg tttgccgcaa
     3541 atgaactcat catgacagta agcgctgtta ttgttttatt cattgtaaca gtctcccgtt
     3601 cgttacagtt ttattgacac accaatacga taactatttt cagataaatt atctttgccg
     3661 ttgtaaacac cctgacggtc aaggtaatca tattctatat atggcgtaat atcgggtgtc
     3721 atatggtatt gcagaacaaa tgcattttca gtcgcatctt tcttatggtt accatagcga
     3781 taattgtttt gtttgctata aacagttgtt tgccatgcaa aggtaaagtc tttatttatt
     3841 ttatacgtta aatatccatc ccaacgatgc gcattgtctc gatccatttt gccagaaagg
     3901 tcttcctgtc tgtaggtttt ccagtcatac cggtaacgga tcccaaggtt caaatctttt
     3961 gtaacatccc acgaaagttt aatatatggt ccatagcgtg tgccattact gctgaagtgt
     4021 gtcagcatcc ctggacgaac agaccattta tcatcaagtt taatggcata attaatttct
     4081 gcttggacat cattcagtgc aagattttct tttttattat catggattgt attccacgta
     4141 ttactttcaa gacttgccca ccatccattc tgccaccctt cgctcacttt taatcgggtt
     4201 tcataggcat gactgccact tctatatcct ccccggatat ccagtgttgc agcattagat
     4261 ataac
//
LOCUS       DAZGBC010000268         4258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGBC010000268 DAZGBC010000000
VERSION     DAZGBC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.270"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..1162
                     /gene="rfaD"
                     /locus_tag="ACHK1J_004867"
     CDS             230..1162
                     /gene="rfaD"
                     /locus_tag="ACHK1J_004867"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO7914013.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1172..2218
                     /gene="rfaF"
                     /locus_tag="ACHK1J_004868"
     CDS             1172..2218
                     /gene="rfaF"
                     /locus_tag="ACHK1J_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO7914014.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2222..3214
                     /gene="rfaC"
                     /locus_tag="ACHK1J_004869"
     CDS             2222..3214
                     /gene="rfaC"
                     /locus_tag="ACHK1J_004869"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO7914015.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIIINKLTSEIR"
     gene            3211..>4258
                     /gene="rfaL"
                     /locus_tag="ACHK1J_004870"
     CDS             3211..>4258
                     /gene="rfaL"
                     /locus_tag="ACHK1J_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO7914016.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKRENIEKIVFFSFISALSLRCFSELIAYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSFIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVVDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECI"
BASE COUNT         1118 a          959 c         1045 g         1136 t
ORIGIN      
        1 ttcaaatgct tccgttattt accatacatc ggaatattga tactaaagca ctattcacgt
       61 gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg ttgcaattag
      121 catccttgca tctctatgta aagggctgaa gggattcgga tgtgatggta tgattacaga
      181 cattcatgtc tgagattgtc tctgactcca taattcgaag gttacagtta tgatcatcgt
      241 taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg ataaaggcat
      301 caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga acctggtgga
      361 tctggatatc gcggactata tggataagga agacttcctg atccagatta tggctggcga
      421 agagttcggc gatgtcgaag cgattttcca cgaaggcgca tgctcttcca ccaccgagtg
      481 ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc tgcactactg
      541 tctggagcgc gaaatcccgt tcctgtatgc atcttccgcg gccacctacg gcggacgcac
      601 ctccgacttt attgaatccc gcgagtacga aaaaccgttg aacgtctacg gttactcaaa
      661 attcctgttt gatgaatatg ttcgtcaaat cctgcctgaa gcgaactcgc agattgttgg
      721 cttccgttat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca tggcgagcgt
      781 cgctttccat ctcaacaccc agcttaacaa cggtgaatca ccgaagctgt ttgaaggtag
      841 cgagaacttc aaacgcgact tcgtctatgt gggcgacgtg gctgatgtga atctgtggtt
      901 cctggaaaat ggcgtttccg gcatcttcaa tctcggtact ggtcgtgcgg aatccttcca
      961 ggctgtagct gatgctacgc tggcttatca caagaaaggc cagatcgaat acattccgtt
     1021 cccggataaa ctgaaaggcc gctatcaggc gttcactcag gcagatctga caaatctgcg
     1081 cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg aatacatggc
     1141 ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg gcccgtcttg
     1201 ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc gctatcccca
     1261 ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc ggatgccgga
     1321 agttaacgaa gctattccta tgcctctcgg tcacggagcg ctggaaatcg gcgaacgccg
     1381 caaactgggt catagtctgc gtgaaaagcg ctacgaccgc gcctacgtct tacccaactc
     1441 cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg gctggcgcgg
     1501 cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag cctggccgct
     1561 aatggtggaa cgctatatag cgctggccta tgacaaaggc attatgcgca cagcacaaga
     1621 tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa aatcatatac
     1681 ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggtttttgcc cgggtgcgga
     1741 gtttggtccg gcaaaacgct ggccacacta ccactatgcg gagctggcaa agcagctgat
     1801 tgatgaaggt tatcaggtgg ttctgtttgg ctcggcgaaa gatcatgaag cgggcaatga
     1861 gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg cgggggaaac
     1921 acagcttgat caagcggtta tcctgattgc agcctgtaaa gccattgtca ctaacgattc
     1981 tggcctgatg catgttgcgg cggcgctcaa tcgtccgctg gttgccctgt atggtccgag
     2041 tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtt tgattaccgg
     2101 ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct taatcgacat
     2161 tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg aagcctgacg
     2221 gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata cgttgcccgc
     2281 actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg tggaagaagg
     2341 gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg tggcaatacg
     2401 tcgctggcgt aaagcctggt tctcggcccc cataaaagcg gaacgcaaag cgtttcgtga
     2461 agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg taaaaagcgc
     2521 ggcgctggtg acgcgtctgg cgcatggcgt aaagcatggc atggactggc aaaccgctcg
     2581 cgaaccttta gccagcctgt tttacaatcg taagcatcat attgcaaaac agcagcacgc
     2641 cgtagaacgc acccgcgaac tatttgccaa aagtttgggc tatagcaaac cgcaaaccca
     2701 gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg ctggcgaata
     2761 tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag aacactggcg
     2821 agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt ggggcgcgcc
     2881 gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg aagtattgcc
     2941 gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg tagtgtcggt
     3001 ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca cggtttatgg
     3061 accaaccgat ccgggattaa ttggcggata tgggaagaat caagtagagt gtcgttctac
     3121 aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa atcttgaaat
     3181 aataatcaat aagttgacat cggagataag atgacctcaa cattattttt ctctctcgag
     3241 aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttatttat taccacctat
     3301 tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac aataacgaca
     3361 atcgtctatc tctgcaaacg gccaaaacac tatctatcac tatttaaaac atttcttttt
     3421 ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc tccagatgca
     3481 ggtgctagca tgaaggaaat tttcaaagct atcattgaga atactttact atgcacaata
     3541 gcaataccgg tcatattgag agacgaaaaa agagaaaata tcgaaaaaat cgttttcttc
     3601 tcatttatta gtgcgttgag cttacgctgt ttttctgaat tgattgccta ttataaggac
     3661 tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc tgactcgatg
     3721 gtctttttat tccctgcatt gttaaatctc tggcttatta aatcagcaaa ataccgcatt
     3781 tcttttgtgg ttctaagctt tatttttatt tttctgatat taggaacttt atccagaggg
     3841 gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa acaatggaag
     3901 ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt cacacataag
     3961 gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta tcgctatgca
     4021 aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat tggttatggt
     4081 tacggtaacg ttgcatataa agatgtctat aataaacgtg tcgttgatta tccagaatgg
     4141 acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt tggcactggt
     4201 ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga gtgtataa
//
LOCUS       DAZGBC010000269         4170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGBC010000269 DAZGBC010000000
VERSION     DAZGBC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.271"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..1138
                     /locus_tag="ACHK1J_004871"
     CDS             41..1138
                     /locus_tag="ACHK1J_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7914017.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1328..1711)
                     /gene="quuD"
                     /locus_tag="ACHK1J_004872"
     CDS             complement(1328..1711)
                     /gene="quuD"
                     /locus_tag="ACHK1J_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO7914018.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDVVVNNSN"
     gene            complement(1797..1937)
                     /gene="ylcG"
                     /locus_tag="ACHK1J_004873"
     CDS             complement(1797..1937)
                     /gene="ylcG"
                     /locus_tag="ACHK1J_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO7914019.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGYVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1934..2296)
                     /gene="rusA"
                     /locus_tag="ACHK1J_004874"
     CDS             complement(1934..2296)
                     /gene="rusA"
                     /locus_tag="ACHK1J_004874"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO7914020.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2293..2583)
                     /gene="ybcO"
                     /locus_tag="ACHK1J_004875"
     CDS             complement(2293..2583)
                     /gene="ybcO"
                     /locus_tag="ACHK1J_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO7914021.1"
                     /translation="MVDLRKAARGRECQVRIPGICNGNPETSVLAHIRLAGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2576..2746)
                     /gene="ninE"
                     /locus_tag="ACHK1J_004876"
     CDS             complement(2576..2746)
                     /gene="ninE"
                     /locus_tag="ACHK1J_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7914022.1"
                     /translation="MTTPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2746..3201)
                     /gene="ybcN"
                     /locus_tag="ACHK1J_004877"
     CDS             complement(2746..3201)
                     /gene="ybcN"
                     /locus_tag="ACHK1J_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO7914023.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3198..3296)
                     /locus_tag="ACHK1J_004878"
     CDS             complement(3198..3296)
                     /locus_tag="ACHK1J_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914024.1"
                     /translation="MRPQVELLALPQMQKCTCVFLHPTFDLCGGEA"
     gene            complement(3372..3623)
                     /locus_tag="ACHK1J_004879"
     CDS             complement(3372..3623)
                     /locus_tag="ACHK1J_004879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914025.1"
                     /translation="MMSFTKCKLMHSHKTSVNAALLDDTKLVVERDLHFLRELYISSP
                     EEFVGHWVYANEPIFHEHPLSVGLSGIVYHKVIDEIEKS"
     gene            complement(3683..4012)
                     /locus_tag="ACHK1J_004880"
     CDS             complement(3683..4012)
                     /locus_tag="ACHK1J_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO7914026.1"
                     /translation="MDAGVLVLAVQQSPITKQFTDNELCTLAWLWRAGNVMLIAYQNV
                     THLLQDAEHGEAGHFTSIEQEYPRILNRARAILVRETAHVKLQPWQDDKWSRVLPHLR
                     QNVIKQR"
BASE COUNT         1114 a          990 c          907 g         1159 t
ORIGIN      
        1 acatcatttt ttaataatag ttcaaaaaaa agggctcacg atgaaaaaat taacagtggc
       61 aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag ctgaaattta
      121 taataaagac agtaacaagc tggatctgta cgggaaagtt aatgccaagc actacttttc
      181 ctctaacgat gcagatgatg gtgatactac ttatgttcgt ctgggcttca aaggcgaaac
      241 ccaaatcaac gatcagctga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg
      301 cgctgaatct caaggttcct ccaaagacaa aacccgtctt gcatttgcag gcctgaaatt
      361 cggtgactac ggctcaatcg attacggccg taactacggt gtagcatacg acatcggtgc
      421 gtggactgac gttctgccag aattcggtgg cgatacctgg acccaaacag atgtgttcat
      481 gactggtcgc accactggtg ttgcaaccta tcgtaacaac gacttctttg gtctggttga
      541 tggtctgaac tttgctgctc agtaccaagg caaaaacgat cgtagcgatt tcgataacta
      601 caccgaaggt aacggtgatg gcttcggttt ctctgctacc tatgaatacg aagggttcgg
      661 tatcggtgca acttatgcga aatctgatcg taccgacact caagttaatg cagggaaagt
      721 tcttcctgaa gtatttgctt ccggtaaaaa tgcagaagtt tgggccgcag gtctgaaata
      781 tgacgctaac aacatttacc tggccactac ctattctgaa acccagaata tgactgtatt
      841 tgctgatcac ttcgttgcta ataaagctca aaacttcgaa gctgttgcac aatatcagtt
      901 cgatttcggt ctgcgtccgt ccgttgctta cctgcaatct aaaggtaagg atcttggagt
      961 atggggcgat caggacttag tcaaatatgt tgatgtaggt gcaacctatt acttcaacaa
     1021 aaatatgtct actttcgttg attacaaaat caacctgctt gacaaaaatg acttcactaa
     1081 agcactcggt gtaagcactg atgacatcgt tgctgtaggt ctggtttacc agttctaatc
     1141 tgattacgaa aaagatatgt tgcgggaggc tttgcctccc caacatataa gtggctccct
     1201 caagccactt cctttaggag cacaaccttg cttctaacta tataaacctt ctgttatata
     1261 ttacccttta tttttggggg cgttgcaacg ccccattttt aataattttt agtaaacaat
     1321 tggcatatta attagagtta ttaacaacga catccatctc taaccggata tctaatgcca
     1381 ttaacatccc ttcaattatg ccctcagcct tctgtaacct tttcccgata taaccatcag
     1441 agcagcaatg cttacctgcc agtgacatga atgtcatacc gactacataa taatctacta
     1501 ataaatcgtg caaatcgctg ttgttctttt tcagacgggc catgcacccg caaatgatca
     1561 tcgcgtcatc gtcacaacat tgcgggcgag attttacttt tgaaggaatt aatcccttaa
     1621 aaccggcggc aatggacgac caggtcacat cttcatgatt attagccgcc cacgctcccc
     1681 aacgctcaag aaccatctga atatcacgca tcaacttact ccacaaaact cagaccagaa
     1741 cgccaattac aagcaaaaat caacaaaaca gtattagttg attgttatct ctgacttcat
     1801 actcctgctc ctgtcagtgt tttggcgtaa ttcttcagta ttcggtaatc ggtcaaaaca
     1861 taaccgggga aacgatataa gcgcagacgc ccccagcggt ggcgaagaag ttctgccata
     1921 ttaaactcaa acatcattca ttccccattt cggtgatggt cagttccagc ctcccacctt
     1981 tggtaacagg catcttcaca acgcggtaat caacgacctg agcatcatcc agccagaaac
     2041 ctgctttagt gagtgcgtca aaagcggctt tttgcagatt atccaggtca cggcgacggc
     2101 gatccggcat gtggcactca atgcggattt tcacaggcat agccaggccg atatccagca
     2161 ttgcgttttt aatgattcgg gcgacgttat cgcggtatgc ctgcccctct gcgctgatgt
     2221 gcgtgcgccc gcgattatgg cggtaatagc gattattgct cggaggccag ggtaatgtga
     2281 tgctgtaggt attcacgcct taataacccc ctctttcagc cagataacct gtgttctcgc
     2341 cataccttcc agcgcgcatt cttttgcata tccagcgtca acaaaatgcg tgcgacggtc
     2401 gatctcatcg tgacaggcag aacatgcaat ggtggcaatc aggtctggcg gtttggtacc
     2461 ggtgccacac aatccagcca gccggatatg tgccagtaca gacgtttcag ggttgccatt
     2521 acatatgcca gggattctta cctggcattc ccgaccacgc gctgcttttc tcaaatcaac
     2581 catgattcct ccttgctgcc agtcgcaacc attttttatc aaccaggctg gcggtatacc
     2641 ctagcagtgt tggtatttcg gatggcttca gctcaggctt acgcttacga cgatttggta
     2701 ctctgtagat gtgtccgttc atgacacgaa taagcggtgt agtcattacg cctcctgctt
     2761 gtcgcgcagc agctggaact cgcagctctg tggaatagtc aggtggcaac caatattcat
     2821 cgcccaggct tcaaccttac acaggaagac atacatctct ccggtatcaa gatcggaggt
     2881 atggcgtaac gactggatcg tagtgatttc gccggttacg acatcaacca ggtccttggt
     2941 ttcataaccg aggtatgtgt gtttgagagc atcttttacc catgctgcag tagcgaacga
     3001 tttccccctg ctgatgaggt attcactgat ttcgctgtac cacatgtggc tgagtgcatt
     3061 ctgggaaaga ctgcgtcttt cgcgccacgg tttaagcacc atgcgaaagc atttgccgtc
     3121 ctccagataa ggctggatct gctgaccgat agcggtgaag ttaccgcgat gcaatttgat
     3181 accgtcttgt gggaggttca cgcttcacct ccgcagaggt caaacgttgg atgcaaaaaa
     3241 acgcaggtgc atttctgcat ctgtggcaag gcgaggagtt caacttgtgg tcgcatataa
     3301 tttcccaatc atatgcgcag aagtcaccgc agggtgttca ggccaacggt gacggtatta
     3361 ttgcttattg attatgattt ttcaatttca tcaataacct tgtgatagac aataccagaa
     3421 aggccaacag acagtggatg ttcatgaaat attggctcat ttgcataaac ccaatgcccg
     3481 acaaattctt ctgggcttga tatgtataac tcacgaagaa aatggagatc cctctctaca
     3541 actaatttcg tatcgtccaa gagcgctgca tttacgctag ttttatggct gtgcattaat
     3601 ttacatttgg tgaaggacat catggcaaaa gctccttttt cttatgaatc catatgttag
     3661 ctaaattgga gagacacagt agctaacgtt gtttaataac attctgacgc aaatgtggca
     3721 atactcgact ccacttatca tcctgccacg gctgaagttt tacatgtgcc gtttctcgga
     3781 cgaggattgc tcgcgctctg ttgagtatcc ggggatattc ttgctcgatg gaagtgaagt
     3841 gaccagcttc accatgctcc gcatcctgaa gaagatgagt aacgttctgg taggcaatta
     3901 acatcacatt ccctgctcgc cataaccagg cgagtgtgca aagttcgtta tcagtgaatt
     3961 gttttgtgat tggggattgt tgaactgcta gaacgagaac gccagcatcc attggcagtc
     4021 cctatagtaa aaccatagct caggacgctt cgttcaggat agataatttt attgtactta
     4081 cctaactttc ttactatagc acggttgaaa aagtgattat tactcaaaaa taaacctcac
     4141 catcaaccat atatttgaga gtacttatcg
//
LOCUS       DAZGBC010000270         4168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGBC010000270 DAZGBC010000000
VERSION     DAZGBC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.272"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(146..1378)
                     /gene="serA"
                     /locus_tag="ACHK1J_004881"
     CDS             complement(146..1378)
                     /gene="serA"
                     /locus_tag="ACHK1J_004881"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO7914027.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(1634..2293)
                     /gene="rpiA"
                     /locus_tag="ACHK1J_004882"
     CDS             complement(1634..2293)
                     /gene="rpiA"
                     /locus_tag="ACHK1J_004882"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO7914028.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(2349..3245)
                     /locus_tag="ACHK1J_004883"
     CDS             complement(2349..3245)
                     /locus_tag="ACHK1J_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7914029.1"
                     /translation="MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERL
                     GVRLIQRTTRRLALTAAGEQFAVECRALMAQEERARAVVMAAQESPQGMLRVSAPVFM
                     AETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
                     HTQADILVASSQWLARHPQVKAIEDLNHVDGILRLANNGGSQWQLINGEQEITLQPEA
                     RLQSNNLRVMLQAVLGGNGVALLPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGR
                     KGMPAIARYFMDELTTRLANGV"
     gene            3346..3975
                     /locus_tag="ACHK1J_004884"
     CDS             3346..3975
                     /locus_tag="ACHK1J_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFO7914030.1"
                     /translation="MKILHIDSSPMLKHSSSRKLSQTLVEALCQHFPKATIIHRDVGL
                     TPPPHLREETFMALCDKAECGSAQIRQEVNGIHAAIEELASCDVLIIGAPMINHSVSS
                     GLKTWIDQVCQAGLTFRFTSAGAVGLLTDKPTFIVSTRGGIYCDEAHLALDHQESYLQ
                     ATLKLMGITNTHILRAEGVDKTQPGRELAEQNALAQIPELITQVFNHYL"
BASE COUNT         1061 a         1115 c          975 g         1017 t
ORIGIN      
        1 ccccattaaa taaacacaac gcattgatct gactttgatt tattttctgg agcatactcg
       61 caaagtagaa tgcgcaacgg tgtggagaag ggataaaaaa acgggcaagt cagtgacctg
      121 cccgttgatt ttcagagaag gggtattagt acagcagacg ggcgcgaatg gtacccggaa
      181 tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcggcttca atatcaataa
      241 ccacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg acgccctgct
      301 cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg atgtgcatca
      361 gacgacgccc accgtgcagt ggcagcgaga cttccgggaa gttcaccgca gagagcgttg
      421 agccattgtc agaatacttg atcaatttac ccgcaacttc caggccaata ttctcctgcg
      481 cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat tcacacagcg
      541 gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg attgccgccc
      601 ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc acagtaccgc
      661 gcgaagcatt aatcagcagc gagccgggct tcattagtga aatttctttc gcgcccatca
      721 tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc atattcagca
      781 ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttattt tcaatatcat
      841 aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta ccaatatgac
      901 cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc gccagtttgt
      961 tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat agcagcagca
     1021 gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg ttaaataccg
     1081 ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttcctata cagaaacagc
     1141 caatagcgac cagcttttct gcggcgttga tcacgtcttc agttagatgg gtacgggatc
     1201 gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca tccagcgcgc
     1261 ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc gccttttggt
     1321 gcactccttc taccagcaga aacttaatct tgtctttctc cagcgatacc tttgccattt
     1381 acccaatcct gtcttttgaa atgttgtgtg cggatttgcg tccgcctttc aacatatcaa
     1441 aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat atcacgcaaa
     1501 ggtaattctt ggatgaatat gctggtttag aggatttatt tagagcaatc gacaatagcc
     1561 aggtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta acgggggagg
     1621 ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca atcagcgcaa
     1681 cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc gcatttatgg
     1741 cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg atcacgttgc
     1801 cattatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc accagctggc
     1861 gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat ttacccagaa
     1921 tatcaacctg cttggaagcg tctgcaatac agataaattt ttctgcaacc gaagcaatga
     1981 ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg ccgttgattt
     2041 catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca aaaacgtgaa
     2101 tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc ccttcaatct
     2161 ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct gtacctacac
     2221 caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct tttttcaatt
     2281 catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt atatacaaaa
     2341 aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa atatcgcgca
     2401 atggcaggca tcccctttcg ccccgcaaat aaagcataca acggtctggg tatgccgctc
     2461 cacggtgcaa acaggcgcac cagttcaccg ttcgcaagcc cctgtttaca ggcaaattga
     2521 ggcaataacg ccacgccatt cccaccaagt actgcttgca gcatgacgcg aaggttattt
     2581 gactgcaaac gcgcctcagg ctgcagcgta atctcctgtt cgccattaat taattgccac
     2641 tggctcccgc cattgttcgc cagccgaagg atcccgtcga cgtggttaag atcttcaata
     2701 gctttcacct gcggatggcg ggccagccac tgagaactgg caactaaaat atcagcctgg
     2761 gtatgtaatt ttttataatg cagcgtcgag tcagcaagcg cgccggaagc cgtaaccagc
     2821 gcaatatcaa tgccttcagc gaccatatcc accgggcgat taattgccag cagttgcacc
     2881 ttcgccaggg gccaacgggc gataaactca ctgacaaact caccaaacca tgtttcagcc
     2941 ataaacaccg gcgcgctgac gcgcagcatt ccctgtgggc tttcctgggc ggccatcacc
     3001 accgctctgg cccgttcctc ctgcgccatc agcgcccgac attcgacagc aaactgttcg
     3061 cctgccgccg tcagggccag acgccgcgta gttcgctgaa taagccgtac gccaaggcgc
     3121 tcctccagtg cagccattcg tcggcttaat accgatttcg acacgcccgc acgcagcgcc
     3181 gcctggctgt agctgccttc atcggcaatc agggcaaagt agtaataatc attccagtcg
     3241 atcatgattg ttctattttc acaacaatga agttcaatta tgtctattta atcaccatat
     3301 aaacaacagt taaacttctc tcatcgcaga aatgagagca agcccatgaa aattcttcat
     3361 atcgacagta gcccaatgct taagcattca agttcgcgga aattaagcca gacgctggta
     3421 gaagcgcttt gccaacactt tccgaaagcc acaattattc atcgcgacgt tggcctgacg
     3481 ccgccgcccc atttacgtga agaaaccttc atggcgcttt gcgataaagc agagtgcggg
     3541 agcgcacaaa ttcggcagga ggtgaatggg attcacgccg ctattgaaga actggcatcg
     3601 tgcgatgtgc tcattattgg cgcgccgatg attaatcaca gcgtttcatc tggcctcaaa
     3661 acctggatag atcaggtctg tcaggctggt ttaacgtttc gttttacttc cgctggtgcc
     3721 gttgggttac tgacggataa accgacgttt attgtctcga cgcgcggtgg tatctattgc
     3781 gatgaagcac atctggcgct ggatcatcag gaaagttacc tgcaagccac gctgaaatta
     3841 atgggcatta ccaatacaca tattttacgt gcggaaggcg tcgacaaaac ccaaccggga
     3901 cgcgaactcg ccgagcaaaa tgcattagca caaatccctg aacttattac acaagtattt
     3961 aatcattatc tttaatataa aaggagttat tatgtctatt aagcaaaatt tatttgccgc
     4021 gttatttctt tgcactctca caggacacgc atttgccgca accgagatac ataaaccgga
     4081 caataacctt gaacagttat caaaagtgag catgtctaca cacaatagca acccagcagg
     4141 cgttgaaagc caaatcaaag agaaagcg
//
LOCUS       DAZGBC010000271         4135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGBC010000271 DAZGBC010000000
VERSION     DAZGBC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.273"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(177..845)
                     /gene="artM"
                     /locus_tag="ACHK1J_004885"
     CDS             complement(177..845)
                     /gene="artM"
                     /locus_tag="ACHK1J_004885"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO7914031.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(845..1561)
                     /gene="artQ"
                     /locus_tag="ACHK1J_004886"
     CDS             complement(845..1561)
                     /gene="artQ"
                     /locus_tag="ACHK1J_004886"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO7914032.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1568..2299)
                     /gene="artI"
                     /locus_tag="ACHK1J_004887"
     CDS             complement(1568..2299)
                     /gene="artI"
                     /locus_tag="ACHK1J_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO7914033.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2317..3045)
                     /gene="artP"
                     /locus_tag="ACHK1J_004888"
     CDS             complement(2317..3045)
                     /gene="artP"
                     /locus_tag="ACHK1J_004888"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO7914034.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3263..3778)
                     /locus_tag="ACHK1J_004889"
     CDS             complement(3263..3778)
                     /locus_tag="ACHK1J_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7914035.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1055 a         1061 c         1030 g          989 t
ORIGIN      
        1 taaaaaaaga cggacaactc actaagttgt ccgtctttat tataagaatt tatgcactat
       61 gtaggccgga taagacatcc acgccgcatc cggcacaggc accgtgctga tgtctgatgc
      121 gacgctggcg cgtcttatca gacctacaaa ccccccggcg aatgtacgca gctacattaa
      181 ttccgccgtt cgaatgccag tgctttgcgc tcgatcagac gcatcatcag cgtcagcagg
      241 ccgttaacga ccaggtaaat aatccctgcc gcaccgaaca ccattacatc gtaggtgcgt
      301 ccgtacaaca actggctgta tcccatcact tccatcagcg taatggtgta agccagagag
      361 gtacttttga acaccagcac cacttcgttg gaataagaag agagcgagcg tttaaaggca
      421 tacggcagca ggatcgccag cgtatctttt ttgctcattc ccagggcgct acaggactgc
      481 cactgacctt ctgggatcgc acgaattgca ccgtaaaaca gctgcgtggt atacgccgca
      541 ctgttcagcg acaacgcaat cagcgcacat aaccacggtt ctgacaacaa atgccacagt
      601 gccggatact cctgcaacgt cgggaactga cccggcccgt aataaatcag gaagatctgc
      661 accagcagcg gtgtaccggt aaacagcgtg atataacccc gcaccagcca caccagcacc
      721 ggcgttttca gcgtcaggat gatggtaaaa atcaacgcca gaatcagtgc cacaatcagc
      781 gaggcaacgg ttagcgtcag gctggtgtgc agccctttca tcagttcggg taaatactca
      841 aacattagct gggcctccgc tcaaaacgtg tcgcgcgcag gtcaatgcgt ttgagaatgt
      901 actgactgag cagggtgatc accaggtaaa tcgccgccgc cacaatgtac caggtaaatg
      961 gttcctgggt acgagtagcg atgcttttgg tttgtagcat taaatcattc acactaatca
     1021 aactgaccag cgcggtatct ttcagcagca ccagccactg gttaccgagg ccaggcagcg
     1081 catgacgcca catctgcggc atcaccagac ggaaaaagat agccgatttc gacagcccca
     1141 gcgcctgacc agattcccac tggcccaccg gtaccgcttt caacgcgccg cgcagcgttt
     1201 gcgaggcata ggcggcatac agcagtgaca gagcgatgac accacaaagg aacggactca
     1261 cgtcgaagtt ctcaatgtcc atctgcaccg ggatctgcac gaacccaaga ttgatggtga
     1321 agccatccga aagcgtcagc agcagctgtg aggagccaaa atagataaac agcaccacca
     1381 gaatttctgg caggccacgt agaatggtta ccagcgctga acctgcccac gcgacaggac
     1441 gccattttgc cgactcccac accgcaaaga acatcgccag cgccagtccg acaatcaatg
     1501 cacaaacggc aaggccgacg gtcatcccgg cggcgcttgc taaaggaaaa aattcattca
     1561 tcaggaatta cttctggaac catttgttgt agatggtttc gtaagtgcca tctttcttca
     1621 ctttttccag cgcagtgttg agtttctgct gcagttcagt gttgccctga cgtaccgcga
     1681 tgccgaggcc agtaccgaag taatctttat cggtcacttt atcgcccact gccgccagtt
     1741 tcgggttatc tttcagccat tcggttacca ctgcggtgtc accaaatacg ctgtcgatac
     1801 gaccattttg cagatccagt tttgcgttct ggtagctgtc atacggaacg gtggtgattt
     1861 ccgggtgctt atccataatg aacttctggt gtgtcgtccc gttctgaacg ccgacttttt
     1921 tgcctttcag ctgatcaaca ctggtgtatt tgccttgctg acccacaaac agggcagagt
     1981 tgtcatagta cggggtggta aacagcacct gcttttcacg ctccggagtg atatccatgc
     2041 ccgccatcac ggcttctacg cgacggaatt tcaggcttgg gatcaggctg tcaaacgcct
     2101 ggttagagaa agtacaggtt gcatcaatct ctttacacag tgcctgtgcc aggtcgacgt
     2161 caaaaccaac gatctggttg tttgcatcaa tcgattcaaa cggaggatag gaggcttcgg
     2221 tagcaaaacg aatggtttcg gcagctgtgg cggaaagact aaaacctgca attaacgcgg
     2281 caatcagaac ttttttcatt gttgttatcc cgaatcttag tgagagagat agtttttaaa
     2341 tgcttcggtt tgcggctcgg taaagcagct cgcgtcgcct tgttctacga tatgaccatt
     2401 ttccatatac accactcggc tggcggtttt acgcgccact tcaacttcgt gggtgacgat
     2461 cacctgggta atattcgttt ctgccagctc acgaatgatg ctgacgattt gtgcggtaat
     2521 ttccgggtcc agtgcggcgg tcggttcatc aaacagcagt acctgcggtt ccatcattaa
     2581 cgcacgggca atagcaacac gctgctgctg accaccagaa agatgcagcg ggtaacgatc
     2641 gctataaggt ttgagacgca gacgttccag cagtttttct gcacgggcca gcgcctgatc
     2701 tttactcaac cccagtacac ggcagggcgc ttcaatcagg ttttgctgca cggtcagatg
     2761 cggccacagg ttgtattgct gaaacaccat gccaacgtta cgacgcaaat cgcgaatcgc
     2821 tttgtctgag ggtgttttgg tgaaatcgaa atggttgcct gcaatgttga gcgtaccgga
     2881 gcgcggcatc tcaagcagat tgagtacacg cagcagcgag cttttacccg cgccgctggg
     2941 gccaagtaac accagcgttt cgccctgtgg gcaatccagc gtgatatcga acagcgcctg
     3001 atgcgcgccg tagaagcaat taatgccgtt taattgaata ctcattgaca ctcgtatact
     3061 ggcagtctga tagctattga ggtcgaagat agtacctttg acagaataat tatgcaatat
     3121 ttctgcttta aaagttaaaa gcaaagcgca ttattcaata aacatagcac aaaataacgg
     3181 gggcggtggt cggcgagcat aaatgtcggc attcctcacg aaatgccgga caatttacgg
     3241 ggtttattgg ttgatcaagg cgttagcgat tctcgatgga ctgacggagc gtacccgccg
     3301 tggcatgaac gctaccgcct aagtagcgca catcgtcgat cacccaacac tggccttcct
     3361 gaatcattaa cacttcatcc tgccagccct ggtcaccctg cttgagatcc acgcgcaatg
     3421 gaatgttacg ggcatcacga ttagggatag tcgatgcact ggcaacgtgg gcgctgtctg
     3481 gcaaggtggt tcgactggag aacggatcgc tgcttaacaa ttcacgatga ctattatcgc
     3541 ggctggcatc gctaagtagg gttgccagtt tgtcactcaa ataggggcgc agggcggtaa
     3601 tgtcattgct gcgatgttga atgcggtagt catagaattg ttgggccacg ctgtcaggcc
     3661 ccccctggac acaggaaccg gtgcggggac cgttatcttt ataagctggc gtgacggtgg
     3721 tacaggcact cagcagcagt gcacatggaa taagtagaga tagtgaacgg tagcgcataa
     3781 tcatttcctt attagcttgc tgtctgcgtc acactacgac caaacgctat cagattattt
     3841 aatacattgt ttttaataca attctgaaac aatctgaaca cctgattggg tttgagtcca
     3901 ttgctggcta cccactcaag gttgctcgta gctgacttac ttaaattacc cacacaatag
     3961 acagtgtaaa ccatttgtga ggttgagcgt gttatatggc tgaaatgatc atttaaggta
     4021 aatatgatag ttctgaaatg gtggtataaa tcctcctgta gaggttaaga gcatctgcaa
     4081 gaagccagtt tcacatttat aacgcgtgat aaatgaacgc ggttattgtc taatc
//
LOCUS       DAZGBC010000272         4132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGBC010000272 DAZGBC010000000
VERSION     DAZGBC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.274"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..2008)
                     /gene="speA"
                     /locus_tag="ACHK1J_004890"
     CDS             complement(32..2008)
                     /gene="speA"
                     /locus_tag="ACHK1J_004890"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO7914036.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2017..2148)
                     /gene="yqgB"
                     /locus_tag="ACHK1J_004891"
     CDS             complement(2017..2148)
                     /gene="yqgB"
                     /locus_tag="ACHK1J_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO7914037.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2242..2385)
                     /gene="yqgG"
                     /locus_tag="ACHK1J_004892"
     CDS             complement(2242..2385)
                     /gene="yqgG"
                     /locus_tag="ACHK1J_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO7914038.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2496..2747)
                     /gene="yqgD"
                     /locus_tag="ACHK1J_004893"
     CDS             complement(2496..2747)
                     /gene="yqgD"
                     /locus_tag="ACHK1J_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO7914039.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2803..3957
                     /gene="metK"
                     /locus_tag="ACHK1J_004894"
     CDS             2803..3957
                     /gene="metK"
                     /locus_tag="ACHK1J_004894"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO7914040.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1047 a         1127 c         1010 g          948 t
ORIGIN      
        1 ataaatcgac ccgccagcgg aataatctta attattcatc ttcaagatag gtataaccgt
       61 acaaacctgc ctcgaactct tcaaggaact gttgttgcag ttcagcatca agatcggttt
      121 tcttcacttg atcacggaac tgagttagca gcgttttcgg atcgagctgt acatattgca
      181 gcatgtccgc cacggtatcg ccttcgtcag acagttccac ttctacacta ccgtcaggga
      241 agacgaacac gtcaaccgct tcggtatcac cgaacaggtt gtgcatgttg ccgaggatct
      301 cctgatatgc gccgaccata aagaaaccga gcatcggcgg attctctgga tcgtactccg
      361 gcataggcat tgtcgtggca ataccatcac catcgatata gtggtcgata gcaccgtcag
      421 agtcacaggt aatatccagc agcacagcgc ggcgttccgg cacttgatcc agcccttcca
      481 gcggcagaac cgggaacaac tggtcgatcc cccacgcatc cggcatcgac tggaacagcg
      541 agaagttgac gtacattttg tccgccatac gttcctgcag ctcgtcgata atcggacggt
      601 gagcacggtt ttgcggatcc agctgctttt gcacttcatg gcacatgctc aaataaagct
      661 gctcagccca tgcacgttct tgcaggctaa aggtgccgga agagtagccg atatgaatgt
      721 cgtgcagatc catctgactg tcgtgtaacc attcacgcag agaacggcga gttcccggtt
      781 cgtgcatctc ctgccaggtt tcccacatgc tttgcagcgc gcgcggcgca tcttctgcag
      841 gcgcggtcgg caccgtgtat tcgttacgtt ccacgccgat gatattagac accagcacgg
      901 tgtgatgcgc agtcactgca cgacccgatt cagtgattac cgtcggatgc ggcagaccgt
      961 tttcttcaca cgcatcacca atcgcccaga tgatgttgtt ggcgtattca ttgaggccat
     1021 agtttacgga gcagtcagac tgcgaacggg tgccttcata gtccacgccc agaccgccgc
     1081 cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag ttccacatag aaacgcgcgg
     1141 attcacgaac gcccgtggcg atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt
     1201 gcagtagttg caggctgtcg agacgcccgg cttcacgcag ggtttcaacc agttgcagta
     1261 cctgagtcgc agccaggccg aacttcgatt tttccccgcc ggaagactgc catttacccg
     1321 agccctgcga agccagacgt gcacgcacgc ccagacgagg aacgacattc agacgttctg
     1381 cttcatccag cacaatggcg atttctgaca tcttctcaat gaccagatag accttgtgcc
     1441 ccatcttctc gccaattaat gccaggcgga tatattcgcg gtctttataa ccgttgcaga
     1501 cgatgacgct acgggtcatg ccagcatgtg ccagcactgc catcaactcg gctttggaac
     1561 cggcttccag acccagcggt tcgcccgaat gaatcaggga ctcaatcacg cgacggtgct
     1621 ggttaacttt gatcggataa acaaggaagt aatcgccgtt atagccgtag gattcccgcg
     1681 cacgtttgaa cgcggcgtta atggaacgca aacggtgctg caggatctgt gggaaacaga
     1741 acagtgcagg cagacgctgg ccctgtgctt cacgagtttt cactaactgc gcgagatcga
     1801 cgcgagcttc cgggacgtcc gggtccgggc acacgctgat gtggcccagc tcgttaacgt
     1861 catagtagtt attgccccac caggcaatat tgtaagtacg cagcatcttg ctggcttcct
     1921 gggagctcat tgcaacctcc tgcatggagc gtagtacacc gtgttcgccc gctgacgaag
     1981 gcaaacccat agacatgtcg tcagacatag cgaacctcaa attattttat taagtgtaaa
     2041 acagttaacg actatcgcag cctggaactg cgataacaac ccataagcac atggattttc
     2101 cagcagtgaa tgctgacgct ccaactgcgc aaccggtttc tttttcataa cattattaag
     2161 cacataaccg aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc ttgtttttac
     2221 aagagcgccc ttgttcagtc ctcagtaact gtaaccagct cttgaatcct gagaagcgcc
     2281 gagatgggta taacatcggc aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc
     2341 ttcaccagaa cggtgagaca ggttaagcag cagacaacgg ttcattattt cgtatcacct
     2401 ccacggccgc ctgttaagac gaacccacaa gccaaaactc tgatttcaac ccggctggaa
     2461 gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac
     2521 cccacaatgg cagaaaattg caaaagataa atacgcagaa tgccggcatt gtcaggaaaa
     2581 atttccagcc acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg
     2641 aagaattgac ctaaaatagc catccagatg ttaatccatc cataccgatt aacactcaga
     2701 ctgccagtgt ttttaacctg cagagtcgtg gtaggatccg ctaccacaga aaatccacac
     2761 aacagtttga gctaaccaaa ttctctttag gtgatattaa atatggcaaa acaccttttt
     2821 acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat ctctgatgcc
     2881 gttttagacg cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta
     2941 aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa
     3001 gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtac attccgacat gggctttgac
     3061 gctaactcct gtgcggttct gagcgctatc ggcaaacagt ctcctgacat caaccagggc
     3121 gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc agggtctgat gtttggctac
     3181 gcaactaatg aaaccgacgt gctgatgcca gcacctatca cctatgcaca ccgtctggta
     3241 cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa
     3301 agccaggtga ctttccagta tgacgacggc aaaatcgttg gtatcgatgc tgtcgtgctt
     3361 tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag
     3421 atcatcaagc cgattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac
     3481 ccgaccggtc gttttgttat cggtggcccg atgggtgact gcggtctgac tggtcgtaaa
     3541 attatcgttg atacctacgg cggcatggca cgtcacggtg gcggagcatt ctctggtaaa
     3601 gatccatcaa aagtggaccg ttccgctgcg tacgcagcac gttatgttgc aaaaaacatc
     3661 gttgctgctg gcctagccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct
     3721 gaaccgactt ccatcatggt agaaactttc ggtaccgaga aagtgccttc tgaacaactg
     3781 actctgctgg tacgtgagtt cttcgacctg cgcccatacg gtctgattca gatgctggat
     3841 ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc
     3901 ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg ctgccggtct gaagtaatct
     3961 ttcttcacct gctttcaaag gccagcctcg cgctggcctt tttcttttac ctcaccgcgg
     4021 tggataggcg ttcacgccgc atccggcaaa aaaaccgcct gcacaataac atcattcttc
     4081 ctgatcacgt ttcaccgcaa attatcatca caactgaaac cgattacacc aa
//
LOCUS       DAZGBC010000273         4114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGBC010000273 DAZGBC010000000
VERSION     DAZGBC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.275"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..117
                     /locus_tag="ACHK1J_004895"
     CDS             <1..117
                     /locus_tag="ACHK1J_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914041.1"
                     /translation="IEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            133..1470
                     /gene="tssK"
                     /locus_tag="ACHK1J_004896"
     CDS             133..1470
                     /gene="tssK"
                     /locus_tag="ACHK1J_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7914042.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSDRSLVDIVLA
                     LPLLNANGGNLDDGRESGRPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQKGVMWEGAL
                     HDARLREGADFWLSVRSSMPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            1467..2120
                     /gene="tssL"
                     /locus_tag="ACHK1J_004897"
     CDS             1467..2120
                     /gene="tssL"
                     /locus_tag="ACHK1J_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO7914043.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVK
                     QAREELAEAGFSQENSDTMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIMTLAGLWCVLSSVLADQVARLTGQG"
     gene            2123..3823
                     /locus_tag="ACHK1J_004898"
     CDS             2123..3823
                     /locus_tag="ACHK1J_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7914044.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSRLLNDWQ
                     QHGVPQRYGLGLYQGMWLIPPVERATGEWVAPLPPRPVIQKVTQTPKTVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQVDACRLPDITRAP
                     "
     gene            3858..>4114
                     /gene="hcp"
                     /locus_tag="ACHK1J_004899"
     CDS             3858..>4114
                     /gene="hcp"
                     /locus_tag="ACHK1J_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO7914045.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSA"
BASE COUNT          813 a         1051 c         1272 g          978 t
ORIGIN      
        1 atcgaagaca acccgggatt tttccgcgtg aaactctatg ctgtaccaca tttccaggtg
       61 gaaggtatgg acgtcagtct gtcactggtt tcgcagatgc caaaggcaaa agcgtaaggc
      121 aggcgtcggg aaatgaagat ttatcgtcca ctgtgggaag acggggcttt tctgatgccc
      181 cagcagtttc agcagcaggc tgcctgggat gttcatctgg cagacagcgt tgcccgaatg
      241 gggctggcgc atccctgggg cgtggtggct gcagagtttg atgactctct gttaccgctt
      301 tcacggttga atgccacccg tctgattgtt cgtttcccgg acggaacgct tattgatacg
      361 gaacgtgcgg ataatctgcc tccggtctgt gatttatcga cggtttctga tcgttccctg
      421 gtggatattg tgctggcgct accgttactg aatgctaacg gtggcaacct tgatgatggc
      481 cgtgagagcg ggcgtccgcg tcgctggaaa tcagaaaggg tgaatgtgca ggagctggcc
      541 gggcatgagc aaagcgaagt ggcggtattg cggcataacc tgactctgcg catggctcat
      601 caggaaaatg ctgcctggct gacctgtccc gttacccgtc ttgttcgtga tgcacaggga
      661 cagtggtgca gggacccgcg ttttattccc cctctgctga cgctgtctgc cagtccttcg
      721 ctgatgactg agcttgcgga gttgcttcat cacctgcagg cgcgtcgcca gcgactgatg
      781 tccatgcgtc gggagaacaa tgcccgtctt gcggattttg cagtggcgga cgtgtcgttg
      841 ttctggttgc tgaatgccct gaacagtgcg gagcctgttc tgaaagagtt gcttgacatg
      901 ccttaccgcc acccggaact gttgtacagg gagcttgccc ggctggcagg ctccctgctc
      961 acattctcgc ttgagcataa tgtggatgcg gttccggcat accgtcatga aacacctgaa
     1021 aatgttttcc cacctttgct ttctttgctg aaccagctgc tggaggcaag cctgccttcc
     1081 cgtgtggtct tcattgaact gaaacaaaaa ggtgtgatgt gggagggggc attacacgat
     1141 gcgcgcctgc gcgaaggagc ggatttctgg ctttcagtgc gctcttccat gcccgggcat
     1201 gaactgcaga cgaagtttcc gcagctatgc aaagccggga gccccgatga tgtgtctgag
     1261 gtggtgaatg tggcgctgag cggggtcatt atccgtccgg ttacgcatgt accggcggcc
     1321 attccgctcc ggctggaaaa ccagtatttc gcgctggatt taagtacgga tgcggcccgg
     1381 gcgatgctgg acgcggggcg ttgtactttc tacaccccgg catcgctggg agatgtgaaa
     1441 ctggaacttt ttgcggtgct gcggacatga ataaaccagt tatttcccgg gctgaacaga
     1501 ttttttatcc cggctggctg atggtctgcc agctgcgcag cggacaaccg gttgaggatg
     1561 gtaaggccct gtatcgccgt gcctgtcagc tggtgaaaca ggcgcgtgaa gagctggccg
     1621 aagcaggatt cagtcaggaa aacagtgaca ccatgttgta tgccttctgt gccctgctgg
     1681 acgagagtgt gctgaaccgc gaaaaaacag acgatggctg gcacacctgg cagcaggacc
     1741 cgctgcaggc tcattttttt ggtacgctca atgccggtga agagctctgg gagcgtattc
     1801 gtgagcaact gaaattacca gcgccggatg tggcggtact gacatgcctc tgccgtacgc
     1861 ttcagctcgg ttttaccggt cagtaccggt cgcaggatga tgagcgtcgc gaagatgtaa
     1921 tacgcgcact gacagcgcgt gttcctgcat ttactttcgc acaggatgcg ccggtggtgg
     1981 tgcgtgcacc ggggtaccgt gctggcagaa cgatgtactg gttgtcatgg gtggcgggta
     2041 ttatgacgct ggctggtctc tggtgtgtgc tttcttctgt gctcgcagac caggtggcac
     2101 ggcttaccgg gcagggataa ccatgcacgt acgttcccgt ctctggctga caatactgag
     2161 tctgttcctg acactgtggc tgatttcggg attcctgcca ctgtctgcag taaaccagat
     2221 aatactgacc ctggtttcag ttgtggcggc ggggcttgtg ctgtggtatc agtggcgttg
     2281 tctgtcttcc cgtcgtgata tcccggagaa tgccggcgat gacggtctgc cgcctgagta
     2341 ttttcagggg catgtgttgc ttgttgccgg aaactcagat gcgtggttcg aacccggtca
     2401 ggcataccgg gaaacagcat cgggatggta tcttcgtgtg gatacgccgg agcagatgag
     2461 aatactggca gagcggttgt cgtacagccg tccggccctg tgtggccagg tcgtggtcat
     2521 gctgggactt ttaccggagc agcatacctc agatgaagct ttcctgtcgt ggttgcggaa
     2581 ctggcagcgt gcagtcatgc agtgccggca gtggctgaat accatgccgc cggtgctggt
     2641 gacattgatg gtttcagggc cgtcaccaca aaacacatcc ttaaataagc actctgcaca
     2701 ctggtttaca ctgacaccgg acctgtcggg aatgcacata cgcctgacgg aagccggagc
     2761 cattccggca gaggaatggc tccgggagtg tgatgcagcg tccaggctcc ggtgcgtcag
     2821 tgaactgtta tggctgaatg ccatgttgtc ctggtacaac cgggttactg atgccctgac
     2881 agatatttgc cggcaggagc acttttattt ccggccggtt gccgttggat tctgtctgac
     2941 gtcggttgcg gcccggcccg gtaatctctg gcaacaacaa ctgacgtcac tcactgtact
     3001 gccaccgcct gccggcgtgt catcacaggc actgccgttg ccggatattc tgctgtccgg
     3061 tattccccgt catcgtggac tcagtcactg gcacagactt tgtctccaga cgggggcgat
     3121 tgtcggcgtt tttcttctgc tggctatgct ggcgtctttt atgaataacc agcgactggt
     3181 tcgcagcgtg ggggaacacc tgacggcctg gcaccggatg cgcggatatt catctgaatc
     3241 gaaaaaaact gcactgcaac gtcttcaggc tgacagccgg ttactgaacg actggcagca
     3301 acatggtgtg ccgcagcgtt acggactggg attgtatcag ggaatgtggc tgataccgcc
     3361 ggttgagcga gcgactggtg agtgggttgc accactgcct ccccgtccgg tgatacagaa
     3421 agttactcag acgccgaaaa cggttcgcct taacagcctg gcgctgtttg atgccggtaa
     3481 atggacgctg aaaccgggcg ccacaaaatg gctggtgaat gccctggtgg atattaaggc
     3541 aaaagcgggc tggctgattg tggtcagtgg tcacacggac aatacgggtg acccgcagcg
     3601 caaccaggcc ctgtcactga agcgcgcaga ggccgttcgc gactggatgc gggatacggg
     3661 cgatatccct caaagctgtt ttgccgtaca gggatatggt gagagtcgtc ccgtggctcc
     3721 gaatgagacg gcggaagggc gagcgcgtaa ccgccgtgtt gaaatcagtc tggtaccgca
     3781 ggtcgatgcc tgtcgtctgc cggacatcac ccgggcgccg taacagggcg cgtaagacaa
     3841 cgagagggag taactttatg gcaattcctg tttatctgtg gctgcaggat gatggcggag
     3901 cggatattaa aggctccgtg gatgtgaagg accgtgaggg cagtattgaa gtggtggctc
     3961 aggagcactg cctttacatt ccgacggata acaataccgg taagctgacc ggtacccgta
     4021 ttcacacgcc gttcctgttt accaaggaaa ttgattcttc cagcccctac ctttacaagg
     4081 cggtgaccac ggggcagacc ctgaagtctg ctga
//
LOCUS       DAZGBC010000274         4069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGBC010000274 DAZGBC010000000
VERSION     DAZGBC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.276"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..93
                     /locus_tag="ACHK1J_004900"
     CDS             <1..93
                     /locus_tag="ACHK1J_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914046.1"
                     /translation="LEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            90..400
                     /locus_tag="ACHK1J_004901"
                     /pseudo
     CDS             90..400
                     /locus_tag="ACHK1J_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            477..548
                     /locus_tag="ACHK1J_004902"
                     /pseudo
     CDS             477..548
                     /locus_tag="ACHK1J_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
     gene            complement(630..2330)
                     /gene="ltrA"
                     /locus_tag="ACHK1J_004903"
     CDS             complement(630..2330)
                     /gene="ltrA"
                     /locus_tag="ACHK1J_004903"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO7914047.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWARYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            2957..3568
                     /locus_tag="ACHK1J_004904"
                     /pseudo
     CDS             2957..3568
                     /locus_tag="ACHK1J_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            3569..3790
                     /locus_tag="ACHK1J_004905"
     CDS             3569..3790
                     /locus_tag="ACHK1J_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914048.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            3720..>4069
                     /locus_tag="ACHK1J_004906"
     CDS             3720..>4069
                     /locus_tag="ACHK1J_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7914049.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKY
                     PGDTPLMLVIWSPEEIQALSDGMDIALTDHEIRTVLARLEDIPEDQRTESGISSGVAM
                     EIINNVSENRQVTVP"
BASE COUNT          838 a         1111 c         1074 g         1046 t
ORIGIN      
        1 ctggaatggt cttccccgac agatgacgat gagctgccgg aagtcaccgg acctgcgtgg
       61 atagtcgaca atgtggcgca ttatggcgga tgaagtatga cggagacggg cgggcaaccg
      121 cccgtttctt ttccgacaaa ggatgtcgcc gtgctcctct ttttactgcg ccggctgacg
      181 cggcgcggca ggaacgctgc ctgtggtcag tgtcccgcgt ccggcgggca cgggacgggc
      241 gcttttaccg ctcccggctg gcgtcggtga cagtgtacgc cagcccgtcg cccttttctg
      301 atgaacgccc ttcaagccgc tttcgcggca taaccttgcc gtcagaaaga cgacggcaag
      361 gtattccacg gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggg
      421 aacgggcgcc acaggggcgc cctcccggtg ctctctgtaa gagaaggagt gttgttatgt
      481 cccgttttat cctgggtgac tgcgtgcgtg ttatggccac gtttccgggc aatgctgtcg
      541 atttcatgtc gggtagcagc tgcacattac tgtgcagccg ccccctaaga accgtacgtg
      601 ccagtttccc ggcatacggc tcaagccttt caataactcc acattagagc cggcttcata
      661 acagatattc cctgatgttt tgcagtgtga tgacatgccg gatgtaacat ggccagatta
      721 cctatgttat ccagtccccc ttcggatttc ggcacaatgt gtcgaagttg ccagcctgtt
      781 gtggcgttca ctggcaggtg gcagacgggg cactgacctc cctgtcggag ccacagattt
      841 cttaggcgag agtaaccctg tatggattcc agcatcgtag cttttagccg tgcttcaaag
      901 tacgattcgt agctgggatc aaaagggtta gcagtgcttt ttatttgtct gtgtcttttg
      961 actggcatat ccgagatttc atatagcgcc agtaactcac gtttgcctga tggcaggagc
     1021 ttccctgttt ctgtgccgaa tacccagtgt cggaagttaa ctgaacgaaa gtatttcttt
     1081 cttatccagc ctgtaccttt ctttgggtgt cgtcgacatg cccagcgcca tagtgctttc
     1141 catatttcat gactgacttt ctgaaagatc ttttttgaaa caacatgacg atagtatctg
     1201 gcccagcctt tgaggactgg gttgagatgc catatcagtg tgctctggct gactgtcgga
     1261 ttgtctttta tcgtctggcg gacccttttc aggaattcct ttaccttttg gggtgaaggt
     1321 ttaatcaaca gtttcccgtt atatttgcgg atgttcatac ccagaaagtc gaatccatca
     1381 ctgatgtgtg tgattcgtgt cttttctggt gagagggtaa gccctctttc tgccagaaag
     1441 ttttctaaca agggaagaac cttttctgac agcgtgtcgt ggcttatccc cgtgatcaca
     1501 aaatcgtccg cataacggat tatgttgatc ttttctggat tcagtccgcc tcgatagaag
     1561 gttttcttca acagaggctg cagaccatcc agaaccatgt ttgccagcgt tggagatatt
     1621 attccgccct gtggagttcc tgcactggtt gggtataatt tccccaaatc aatatatccg
     1681 gctttcagcc atcgtttcag gatcgccgta tccgttggga tattggcgat tagccattca
     1741 tgtgaaatct catcaaaaca actgcgaatg tcggcttcca gaatccattg tgcccgatca
     1801 gccctgtgca acgttgtgaa acattgctca atggcatcgg ctgttgagcg acagggcctg
     1861 aacccaaacg aatttgggtc agccgttgtt tctgcaacag gttccagcgc catcagatag
     1921 agtgcctgca tggctctgtc tttcattgtc gggatcccta atggtcgctt tttgccgtta
     1981 gattttggga tatatatacg ccgtagcgga agaggtttgt aaccccttct tttcagcgtg
     2041 tatatagcct tcatcttgct gccgggtttt gaccatgttt tgccatcaac acccggcgtt
     2101 ctcttccctc tgttatctgt cactcgttta actgccattg ctctggcgct gaacgagtgg
     2161 gtgagtagcc attgcaaggc ttttaccttg cccgctttct cctcctgtat tgccttcacg
     2221 atacgcgcct gcagccttct gacctcgcga cggcacttgg cccagttgat attgtgccag
     2281 tgagtcacga tgtcggtggg tgcacccgcg tttatgtttt tcgcagtcat ctgcttaccc
     2341 tccttcaaag gtattcaggt tatctcgtgt tgaaagacca tgcggaagtc tgcccgcttt
     2401 cgcgtggaat gatgttgctg aaacagctca actcctgtcc atcccattac aggatggcat
     2461 tcgctttctc cgcactcctg tacccgcatc actgacagct ttcctcgcgg ttagcctgcc
     2521 ctgagggcag tgatacgggc ttaccgtgtt ccgcacagtt tgcagggatg ggatgggtta
     2581 ggttctgcct atccaccggt agctttgatg tccgtgcctc cccagtcaaa agaggaaggt
     2641 ccagctacgt tcccattttg ggtcgggcct gtcagtgcct ttggcccgtt cagggttgac
     2701 gatgtttatc agcagttcac atgtgttaac cctgccatcc agccttgcgc cccatccgcc
     2761 ctgacactgg cagacatgct tcctcctcgc ggattccaca tggtgtgaaa accgggctac
     2821 gttgtcccga aggcttcata ccgaacagtt acccgctctg catgcttcgg taggctacag
     2881 gcgacggaac gcctggtttt actacttcct ttacatgaag ttaaaacaac aaactgtgcg
     2941 actttcacgt cgcacactga ctgacccgcc gtacctcgtc ggttttcgtg accgtttcgg
     3001 gcgcaccatc gctggcgata aaaccgatga atggctgcaa ccggcctgta atgaaatgta
     3061 ccgcgtactc aaaaaagacg cgctgatgat cagcttctac ggctggcacc gtattgaccg
     3121 ctttatggcg gcctggaaac gcgccggatt cagcgtggca gggcacctcg tgttcaccaa
     3181 aaactacaca tccaaatctg cgtatgtcgc gtaccgccac gaatgcgcgt acatcctggc
     3241 aaagggcagt ccggcccggc cccgtaaccc gctgccggat gtgctgggct ggaaatattc
     3301 gggcaaccgc catcacccga cggaaaagcc cgttaccagc ctgcaaccac tgattgagag
     3361 cttcacacat ccgaacgcga ttgtgctgga cccgtttgca ggcagcggct caacctgcgt
     3421 cgctgccctc cagtccggac gccggtatat cggtatcgag ctgcttgaac agtatcaccg
     3481 tgccggacag caacgactgg ccgccgtgca acgggccatg cagcaggggg ccgcgaatga
     3541 taactggttt gaaccggagg ctgcgtaaat gaactatgcc ggacacgaaa aacttcgcgc
     3601 cgacgtggcg gaggtggcca acaccatgtg tgaccttcgg gccaggctga acgatatgga
     3661 gcatcgctgt cgctttgatt ccgatgtgct tgttgaacgc ctgacacgcc agacgctgta
     3721 tcgcgccaat cgcctgttca tggaggcgta taccgaaatt ctggaactgg attcctgctt
     3781 caaagattga gaaggagacg agaatgtacg gaacatgcga aacgctatgc cgggagctgg
     3841 cagcaaagta tccgggagac acgccactga tgctggttat ctggtccccg gaagaaattc
     3901 aggccctctc tgacggaatg gatattgccc tgaccgatca tgaaatcagg actgtcctgg
     3961 cgcgcctgga ggacatcccg gaagaccagc ggactgaatc cggtatttct tccggcgtgg
     4021 cgatggagat catcaataac gtgagcgaaa accgccaggt gaccgtccc
//
LOCUS       DAZGBC010000275         3936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGBC010000275 DAZGBC010000000
VERSION     DAZGBC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.277"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..3219
                     /locus_tag="ACHK1J_004907"
     CDS             <1..3219
                     /locus_tag="ACHK1J_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7914050.1"
                     /translation="ISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTI
                     TSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQ
                     YLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALFGV
                     QVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKD
                     RHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLI
                     KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLDRE
                     ITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTLRQ
                     RLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTA
                     EVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLA
                     LGNYRLTVRAANAWGQQGDPASASFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVDLTEDNASKLEQFSKEWKDANDK
                     WNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMF
                     VAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGTLN
                     NVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMI
                     PPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAG
                     QGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            3286..3885
                     /locus_tag="ACHK1J_004908"
     CDS             3286..3885
                     /locus_tag="ACHK1J_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7914051.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          929 a         1002 c         1200 g          805 t
ORIGIN      
        1 atatccggcg tcacggtggt gttccgggcc ggtgagcagg agcagacacc gccggaggga
       61 tttgaatcct ccggctccga gacggtgctc ggtacagaag tgaaatatga cacgccgatc
      121 acccggacca tcacgtcggc aaacattgac cgtctgcgtt ttactttcgg cgtgcaggca
      181 ctggtggaaa ccacctcaaa gggggacagg aatccatcgg aagtccgcct gctggttcag
      241 atacaacgta acggtggctg ggtgacggaa aaagacatca ccattaaggg taaaaccact
      301 tcacagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt cagtatccgg
      361 atgcgcagga tgacgccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg
      421 tcatacaccg aaattatcga tgtgaaacag tgctacccga acacggcact gttcggcgtg
      481 caggtggact cggagcagtt cggcagccag caggtgagcc gtaattatca tctgcgcggg
      541 cgtattctgc aggtgccgtc gaattataac ccgcagacgc ggcaatacag cggtatctgg
      601 gacggaacgt ttaaaccggc atacagcaac aacatggcct ggtgtctgtg ggatatgctg
      661 acccatccgc gctacggcat ggggaaacgt cttggtgcag cggatgtgga taaatgggcg
      721 ctgtatgtca tcggccagta ctgcgaccag tcggtgccgg acggctttgg cggcacggag
      781 ccgcgcatca cctgtaatgc gtacctgacc acacagcgca aggcgtggga tgtgctcagt
      841 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg
      901 caggaccgac catcagataa ggtgtggacc tataaccgca gtaatgtggt gatgccggat
      961 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgccgttgag
     1021 gtgaactgga ttgacccgaa caacggctgg gagacggcga cagagcttgt tgaagatacg
     1081 caggccattg cccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc
     1141 cgggggcagg cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag
     1201 accgtggact tcagcgtggg tgcggaaggg cttcgccatg taccggggga tgtcattgaa
     1261 atctgcgatg atgactatgc gggtatcagc atcggcgggc gcgtgctggc ggtgaacagc
     1321 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg taccacgctg
     1381 ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac
     1441 ggcgtgaagg tgaaagtgag ccgtgttcct gacggcgttg ccggatacag cgtgtggggg
     1501 ctgaagctgc cgacgttgcg ccagcgcctg ttccgctgtg tgagtatccg tgagaacgat
     1561 gacggcacgt atgccatcac tgccgtgcag catgtaccgg agaaagaagc catcgtggat
     1621 aacggggcgc actttgacgg cgaccagagc ggaacggtaa acggtgtcac gccgccagca
     1681 gtgcagcacc tgaccgcaga agtcaccgca gacagcggtg aataccaggt gctggcgcgc
     1741 tgggacacgc cgaaggtggt gaagggtgtg agctttatgc ttcgcctgac cgtggcagcg
     1801 gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac ataccgcttc
     1861 acgcagctgg cgctgggaaa ctacaggctg acagtccggg cggcaaatgc ctggggacag
     1921 cagggcgatc ctgcatcggc atcgttccgg atcgccgcac cggcagcgcc gtcgcggatt
     1981 gagctgacgc caggctattt tcagataacc gccacgccgc atcttgccgt ttatgatccg
     2041 acggtacagt ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa
     2101 accacagccc gctatcttgg tacggcgctg tactggatag ccgccagtat caatatcaaa
     2161 ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa atcggcattt
     2221 gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaaggc
     2281 cagataaccg aatcccatct cggcaaggag ctgctggaaa aagtcgacct gacggaggat
     2341 aacgccagca aactggagca gttttcgaaa gagtggaagg atgctaacga taagtggaat
     2401 gccatgtggg gcgtcaaaat tgagcagacc aaagacggca aacattatgt cgcgggtatt
     2461 ggcctcagca tggaggacac ggaggaaggc aaactgagcc agtttctggt tgccgccaat
     2521 cgtatcgcgt ttattgaccc ggcaaacggg aatgaaacgc cgatgtttgt ggcgcagggc
     2581 aaccagatat tcatgaacga tgtgttcctg aagcgcctga cggcccccac cattaccagc
     2641 ggtggaaatc caccggcatt ttccctgaca ccggacggaa agctgaccgc taaaaatgcg
     2701 gatatcagtg gcagtgtgaa tgcgaactcc gggacgctca acaacgtcac gattaatgag
     2761 aactgtcaga ttaaggggaa actgtcagcc aaccagattg aaggcgatat tgtcaaaacg
     2821 gtcagcaagt ctttcccccg cacgagcact tatgccagcg gcaccatcac ggtaagaatc
     2881 agtgatgatc agaaatttga ccggcaggtc atgataccgc cagtgttatt ccgcggtggt
     2941 aagcatgaga atttcaacag taataaccaa cagtcatact ggtattcaac ctgccggtta
     3001 agagtgaccc gcaatggtca ggagattttt aatcagtcca cgacggatgc tcagggcgta
     3061 ttttcctcag ttatagatat gcctgccgga caggggacgc tgacactgac attcaccgta
     3121 tcttcatcag gagcgaataa ctggacacca acaaccagta tcagcgatct gctggttgtg
     3181 gtgatgaaaa aatccacagc aggtatcagt atcagctgaa ttttataacc cagaacgggc
     3241 gtcagaaatg acgccttttt tattgcagaa aagcgagagg taattatgcg taaactttat
     3301 gccgccattt tgtccgcagc catttgtctg gccgtatccg gtgcgcctgc atgggcatct
     3361 gaacatcagt ccacgctgag cgcggggtat cttcatgccc ggacgaacgt tcccggcagt
     3421 gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac gctgggaatg
     3481 gtgacgtcat tcagctatgc aggagacaag aatcgccagc ttacccgtta cagcgatacc
     3541 cgctggcatg aagattccgt gcgtaaccgc tggttcagcg tgatggcggg gccgtctgta
     3601 cgcgtgaatg aatggttcag cgcgtatgcg atggcgggtg tggcttacag ccgtgtgtcg
     3661 actttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca cgatgtgctg
     3721 accggaagtg atgacggtcg ccacagcaac acgtctctgg cgtggggggc tggcgtgcag
     3781 tttaacccga ccgaatccgt ggccattgat gtcgcttatg aaggttccgg cagtggtgac
     3841 tggcgcactg acggtttcat cgtgggtgtc ggttataaat tctgattagc caggtaacac
     3901 agcgttatga cagcccgccg gttcaggcgg gctttt
//
LOCUS       DAZGBC010000276         3921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGBC010000276 DAZGBC010000000
VERSION     DAZGBC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.278"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..279
                     /locus_tag="ACHK1J_004909"
     CDS             <1..279
                     /locus_tag="ACHK1J_004909"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7914052.1"
                     /translation="KVACEVITSKPVIMRAYSMTSSTLASRIKERRKALGITQTSLAE
                     SVGMRQQSVQYLESGRATRTGFILELAKVLKCDPDWLLNGENSEQQKV"
     gene            283..2502
                     /locus_tag="ACHK1J_004910"
     CDS             283..2502
                     /locus_tag="ACHK1J_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFO7914053.1"
                     /translation="MPDNKPWGATPDEWFHFDLVLGMTDRLLPVVSNPCAELSPNSKL
                     SHNFGKTPSRYNGQRKVVGIPDWANYVVTENDFARWSNEPDYGICVRTGDGVVALDCD
                     INDAGMQEIVRNIILSCLGELPPRRWRADSHKCLYLIAVDGDYRKRGHRLEGENKQIE
                     LLAKGQQFVACGTHPAGERIQWDCGLPGEPLKITSEQLESLWQRLADNLPVKDSYTAG
                     AGRQRDLSCVDPSATDDVADWLDANGWTLSVSSDGSRNLKPFRDESEYSNGCSETSIK
                     YFPKGTGGFELGHFKSMHNTDAGLTDADWLDAYGYRVADFEDLTVAEDGDKPEYLPWP
                     KFSRDKNDRIEGTVTNVLLALRRPDLCGVQIRLDEFRDEIILITPRGKNLRLRDEFYT
                     MLHSRLECLGFKKFEEAAIRRAVRLVAYENRFDSLKDWIANLPAWDGVPRIDTFFCRH
                     WGIEANAYTHAVGRYWWTLLAGRALVPGIKGDMAVILVSQQGKNKSEGIRAMAPTPEH
                     YMELDFGKKGEERIREMRGHNVIELGEMRGMDKRGIGDVRVTISTRADRNRGLFREHY
                     VTLPRRCGFIGTSNSDTPLTDTEGNRRWLPMTIPDETDGKFIAQGIADEKIQLWAEAV
                     AVFKRDGIAWENAETLAKSILGDYEVKDEVWMSCVAEWLELPQDLYDMAADGTKKNCE
                     RVPLTSKDVLVEAIGFKAPQVKRSDEMKIAAIMKKLGYKNKQLQSLPGKPRSWEKCK"
     gene            2809..3090
                     /locus_tag="ACHK1J_004911"
     CDS             2809..3090
                     /locus_tag="ACHK1J_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914054.1"
                     /translation="MNTTCARLPSADTLAEQAAKTASGWMSDAVIAIDELFGKGYAKQ
                     HPELVAAFMQTAGLDEAAMHLRGLVLANQRIDDTLEVLSEHIMLLNEPQ"
     gene            3159..3581
                     /locus_tag="ACHK1J_004912"
     CDS             3159..3581
                     /locus_tag="ACHK1J_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7914055.1"
                     /translation="MRRDMQLVLERWGRWAASEEYCSMVDWPSMSVTSRDVTDSGKPG
                     CSDEDGAVIDTCIAHMSMVCPYDDLLILGLRFIGGLSTRAIAEAIDRSHLSVRTSLKA
                     SEAFLEGALTLQGVRLDMDPEVVLPERVVCAQKNMLWL"
     gene            3661..3762
                     /locus_tag="ACHK1J_004913"
                     /pseudo
     CDS             3661..3762
                     /locus_tag="ACHK1J_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            3751..3827
                     /locus_tag="ACHK1J_004914"
     tRNA            3751..3827
                     /locus_tag="ACHK1J_004914"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3785..3787,aa:Arg,seq:tct)
BASE COUNT         1027 a          861 c         1058 g          975 t
ORIGIN      
        1 aaggttgcct gtgaggtaat tacaagtaaa cctgtaataa tgagggctta ctcaatgacc
       61 agttccaccc ttgcttcacg tataaaggag cgccgcaaag ctttaggtat aacccaaact
      121 tcattggctg aaagtgttgg catgcgccag cagtctgttc aataccttga gtccggacgc
      181 gccacacgta ccggtttcat ccttgaattg gcaaaagttc tgaaatgcga tcctgactgg
      241 ctattaaacg gcgaaaattc agagcagcaa aaggtgtaac ccatgcctga taacaaaccc
      301 tggggagcta cgcccgatga gtggttccac ttcgatctgg tgttggggat gacagacagg
      361 cttttacctg tggtgtccaa tccctgtgca gaactgtcgc ctaatagcaa gctgtctcat
      421 aatttcggca aaactcccag tcgctacaat ggccaacgta aggttgttgg tatccccgac
      481 tgggctaact atgttgttac ggaaaacgat tttgcccggt ggtcgaacga acccgattac
      541 ggtatttgcg ttcgtactgg cgacggggtg gtggctctgg actgcgatat caatgacgcc
      601 gggatgcagg agatcgtacg caatattatc ctgtcatgcc tgggggaact tccgccacgt
      661 cgctggcggg ctgacagtca taaatgcctg tacctgattg ctgttgatgg cgactaccgt
      721 aaacgtggtc accgcctgga aggcgaaaac aaacaaatag agctgctcgc taagggccag
      781 cagtttgtgg cttgtggtac ccatccggca ggcgaacgta ttcagtggga ctgcggtttg
      841 cccggcgaac ctctgaaaat tacatctgaa cagctcgaaa gcctgtggca gcgtctggcg
      901 gataacttac ccgtgaaaga cagctatacc gcaggtgcag gacgtcagcg tgacctttcc
      961 tgtgttgatc cttccgcgac agatgatgtc gctgactggc ttgacgcgaa cgggtggact
     1021 ctgtcagtca gctctgatgg ttcccgcaat ctgaaaccgt ttcgggatga gtcagaatac
     1081 agcaacggtt gcagcgaaac atccattaag tattttccaa aagggacggg gggatttgaa
     1141 ctgggacatt ttaaaagcat gcacaacacc gatgcggggc ttactgatgc cgactggctg
     1201 gatgcatacg gctaccgcgt ggcggacttc gaagatttga cagtggctga agatggcgat
     1261 aagcctgagt atcttccctg gcctaagttc agtcgtgata agaatgaccg gatagaaggt
     1321 accgttacca atgtgctgct ggcgctgcgg cgaccggatc tctgcggtgt acaaatccgc
     1381 cttgatgagt tccgggacga gataatactg atcactccac gggggaaaaa cctgcggttg
     1441 cgtgatgagt tttataccat gctgcactct aggctcgaat gtctgggatt caagaaattt
     1501 gaagaggcag cgatcaggcg tgcggtgcgc ctggtcgcgt acgaaaaccg ttttgattcg
     1561 cttaaagact ggattgccaa tcttcctgca tgggatggtg ttccccgtat agatactttc
     1621 ttctgtcggc actggggtat cgaggccaac gcctacaccc acgcagtcgg gcgctactgg
     1681 tggactttgc tcgcgggtcg agctctggtt cctggcatta aaggggacat ggctgtgatc
     1741 cttgtcagcc aacagggtaa gaacaagtcc gaaggtatcc gtgcaatggc tccaacccct
     1801 gagcattaca tggaactgga cttcggtaag aagggagagg agcgcattcg tgagatgcga
     1861 gggcataacg ttattgagct tggtgagatg cgcggtatgg ataaacgggg tattggtgat
     1921 gttcgtgtca ccatatccac ccgtgcagac cgcaaccgcg ggttgtttcg ggagcactat
     1981 gtcactttac cgaggcgttg cggttttatt ggtacttcta actccgatac gccattgaca
     2041 gataccgaag gtaaccgccg ttggttgccg atgacgatac cggatgagac tgacggcaag
     2101 tttatcgctc agggtatcgc tgacgaaaaa atacagcttt gggctgaagc agtcgccgtt
     2161 tttaaacgtg atggcatcgc atgggagaac gctgaaacac tggctaagtc tattcttggg
     2221 gattatgagg tcaaagatga ggtctggatg tcatgcgtcg ctgagtggtt agagttgcca
     2281 caggatctgt acgatatggc tgccgacggc acgaaaaaga actgcgaacg cgtccccctt
     2341 acgtcaaaag acgtattggt cgaggcgatt ggcttcaaag ccccccaggt aaaacgcagt
     2401 gacgaaatga aaattgcagc aatcatgaag aagcttggtt acaaaaataa acagttgcaa
     2461 agtttaccgg gtaagcctcg cagctgggaa aaatgcaaat aactacaagt ttgtagattt
     2521 ttgcaggttg ttactcaacc tactcaacct cacaagggta ggttgagtaa gaaaaatggt
     2581 attaaaatca tgatattact caaccactca acctactcaa ctattttata taaatatacg
     2641 cgtgcgcgcg cacgcatgta tgtaagaatt ttatttttcc aaaaggttga gtaggttgag
     2701 taagagtatg atttttatgt tttttaggtt actcaacctt ttgcccgtaa aaaaggttga
     2761 gtaaagaaaa cttgcagaaa atcacaaata accaaacgaa gtaaaaacat gaatacaacc
     2821 tgtgcaagat taccaagtgc ggatacgtta gcggaacagg cagcaaaaac ggcaagtggg
     2881 tggatgtcag acgccgtaat agcgatagat gagctttttg gaaagggcta tgcgaagcaa
     2941 caccctgaat tagttgctgc ctttatgcaa acagcgggct tagatgaagc tgcaatgcac
     3001 ttacggggac ttgtgttagc taaccaacgg attgatgaca cactggaagt gctttcggag
     3061 catattatgc tgcttaacga gccccaataa acccgatgcg gataaacaga cgtagttctc
     3121 agaaaatttt ttcgtagcaa aacgtagagg ccagcgttat gcgtagagat atgcaacttg
     3181 ttctggagcg gtgggggcga tgggcggcga gcgaggaata ctgttcaatg gttgactggc
     3241 cctcaatgtc ggttacttcc cgggacgtaa ccgatagtgg caaacccggt tgctctgacg
     3301 aggacggagc ggtgattgat acctgcatcg cgcatatgag tatggtttgc ccgtacgatg
     3361 atttgctgat cctggggtta cgttttatag gcggcctctc aacgcgtgca attgctgagg
     3421 cgatagaccg tagtcatctt agcgtacgga catccctgaa ggcgtctgaa gcatttctgg
     3481 aaggagctct gactctgcag ggagtaaggt tggatatgga tcctgaagtt gtactacctg
     3541 aaagggttgt gtgcgcacaa aaaaatatgt tatggttata gcgtctataa ttgtatgctt
     3601 actgctgaaa ggttctgata acaccggaac cttttttctt gtcttctgct gggaaaaatt
     3661 aataaaacag gaaagaaaaa catgacatcg cactggttca gtgtcagcca gtggcagtta
     3721 ccaaacgagg atgattacag aaaacttcat gcgttgttag ctcagccgga cagagcaatt
     3781 gccttctaag caatcggtca ctggttcgaa tccagtacaa cgcgccatat ttatttacca
     3841 ggctcgcttt tgcgggcctt ttttatatct gcgccgggtc tggtgccgta ttacttcagc
     3901 caaaaggaat acctgtatat g
//
LOCUS       DAZGBC010000277         3862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGBC010000277 DAZGBC010000000
VERSION     DAZGBC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.279"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1022
                     /locus_tag="ACHK1J_004915"
     CDS             <1..1022
                     /locus_tag="ACHK1J_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HFO7914056.1"
                     /translation="VIKHKAGLLNLAEELSNVSKACKIMGVSRDTFYRYRELVDEGGV
                     DALINRSRRAPNLKNRTDEATEQAVVDYAVAFPAHGQHRTSNELRKQGVFISGSGVRS
                     VWLRHNLENFKKRLKALEEKVARDGIELTDSQIAALERKASDDEACGEIETAHPGYLG
                     SQDTFYVGNLKGVGRIYQQTFVDTYSKVAHCKLYVTKTPITAADLLNDRVLPFYASQG
                     LPMLRILTDRGTEYCGKVEQHDYQLYLAINDIEHTKTKAMSPQTNGICERFHKTILNE
                     FYQVTFRKKLYGDLDTLQSDLDEWLAHYNNERTHQGKMCNGRTPMETLLDGKRIWAEK
                     NLSQM"
     gene            complement(1082..1573)
                     /locus_tag="ACHK1J_004916"
                     /pseudo
     CDS             complement(1082..1573)
                     /locus_tag="ACHK1J_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            1846..2139
                     /locus_tag="ACHK1J_004917"
                     /pseudo
     CDS             1846..2139
                     /locus_tag="ACHK1J_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2197..2624)
                     /gene="tnpA"
                     /locus_tag="ACHK1J_004918"
                     /pseudo
     CDS             complement(2197..2624)
                     /gene="tnpA"
                     /locus_tag="ACHK1J_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
     gene            2632..3840
                     /gene="insQ"
                     /locus_tag="ACHK1J_004919"
     CDS             2632..3840
                     /gene="insQ"
                     /locus_tag="ACHK1J_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO7914057.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
BASE COUNT         1083 a          849 c          942 g          988 t
ORIGIN      
        1 ccgttatcaa acacaaagcc ggtttgctca atctggctga agaactcagc aacgtatcaa
       61 aagcctgtaa aatcatgggc gtttcgcgtg atacgtttta tcgttaccgc gaactggtcg
      121 atgaaggcgg tgtggatgcg ctgattaatc gtagtcgccg cgctcctaac cttaagaacc
      181 gtaccgatga ggcaactgaa caggctgttg ttgattatgc cgtcgctttc ccggcacacg
      241 gtcagcaccg gaccagcaac gagctgcgta aacagggcgt ttttatctcc ggcagtggtg
      301 tccgttctgt ctggttacgc cataaccttg agaatttcaa aaaacgtctg aaagcactgg
      361 aagaaaaagt agcccgtgat ggcattgagc taactgacag ccagatcgca gcactggagc
      421 gtaaagccag tgatgacgag gcctgtggcg aaatcgaaac ggctcatccg ggatatctgg
      481 gttcgcagga cacgttctat gtaggcaacc tgaaaggtgt cgggcgtatt tatcagcaga
      541 cgttcgttga tacgtactcg aaagtggctc actgtaagct ttacgtcacc aaaacaccga
      601 ttaccgcggc tgacttactg aatgatcgtg tactgccgtt ttatgcgtct cagggcctgc
      661 cgatgctgag aatactgacc gacagaggca cagagtactg tggcaaagta gagcagcatg
      721 attatcagct ttatctggca ataaacgata tagaacacac gaaaacaaag gcgatgtccc
      781 cgcagaccaa cggcatctgc gagcggttcc ataaaacaat cctgaacgaa ttttatcagg
      841 taacgttccg taaaaaactg tacggcgatc ttgatacatt acagtcggat cttgatgaat
      901 ggctggctca ctataataat gagcgaaccc atcagggaaa aatgtgcaat ggacggacgc
      961 caatggaaac gttacttgat ggaaagcgca tctgggccga gaaaaattta agccagatgt
     1021 aatctgacag atacctgtat aaataaccgg taactgtcag atcaggtctg agctaataca
     1081 aattaggcat ttgggcagga agggttacaa cgtatcctat cgtttggccg gtatagtctg
     1141 gagtaccata accaggtccc aaaaccactt cgtgtattgt gtatttactg ttgggttcga
     1201 aggattcgac atcttgcaat aaatatttca gttctgcggt taacctcgca cccgcgtaag
     1261 ctttattgta tttctgggcg tgaatattat ctaatgtagt ttcatggttc catccaaaag
     1321 aattatcacc attcattgtt actccatcgt aagcattaca cttctttgct aacttattga
     1381 atgcttgcca gtcagagaga tctagtttgt ggatctcaac agtacgtctg ccatcgcaat
     1441 tatgatctct tccacgattt gacagtaatg tagcgcgtgc gtattgatga aattttagtt
     1501 ttgctggttt gtcggtggac gaaaaacgag gtaaatatgc ggggaatgaa taaatagttg
     1561 ttggtaatgt cattttattg ttgcctgttt aatattttta caaatatcgc agttgaatgt
     1621 tcaaagaaaa agaattgata ttaataaaaa taagtttgtt ttctgaagtg gtgaaaatag
     1681 cggatggtga aagatattaa tatcaaaaat cgctcattat ggatataacg atgcacccgg
     1741 ggagaaaatg acttccacgc cttacaccgt acattcttgt tgtgcggtat gtaattattc
     1801 tacgctgtcg gtatcggtgt attcgttaat cagcaggtta gccagccaca aaaagccatt
     1861 gagaaaatta ttgattttac atgggattat tatattgcta atccctggtt tttaaaaatt
     1921 gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa aatcacagcg tctcttagaa
     1981 attacccatg cgcacttgca gttaatggaa tccttattgg atgaagggaa gaaacacaat
     2041 atttttaagc cagatatcga cccatttcag gtgaatatta atattgctgc gcttggggga
     2101 tattatttga tcaaccagca tacgcttggc ctggtttatc cggtgcgccg taaaaccccg
     2161 tccttcaggg cggggatata cggcgcggtt ttccacctaa ccaggtgttt gctgttgttc
     2221 aatgtattga cggatggcgg atattggcgc acctccgcag ctactggcaa aatagccagg
     2281 actccacaaa acgcctttgt agtaatacct gactgcaata tctggtcgat ctcgtcgcag
     2341 taacctaccc gatacgcctt tgaggctgtt taccagactg gatatcgcca gtttgggagg
     2401 atagttaatt aacaaacgtg atctggttcg ccatccattt caaccagttc agcttcaaaa
     2461 tcagcacata cctttgaaaa gtaagtgcgt agtttttctg tcgcgtcatg gtcaaaaatc
     2521 tggcgtcggt atctggtgac aaagaccagg tgaacatgca tcaggaaaac acaatgcctt
     2581 ccacgccgga tatcggtttc ttttttcaca gaccaaagta tcatttggcc tgtgaaacga
     2641 ctacaggcat ttaaattcca gttaagaccc ggtggtcaac aggagcgtga aatgaggcgc
     2701 ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg cgcttcagaa tgagaatcat
     2761 gaggccggta ataaatacat cccttacggg aaaatggctt cctggctggt tgagtggaaa
     2821 aatgccactg aaacgcaatg gcttaaagat gccccgtcac agccattgca acagtcactg
     2881 aaagaccttg agcgggctta caaaaacttc ttccggaagc gggctgcttt tccccgattc
     2941 aaaaagcggg gacagaatga tgcattccgc tacccgcagg gcgttaagct cgatcaggaa
     3001 aacagtcgta tttttctgcc gaaacttggc tggatgcgct accggaatag ccgtcaggtc
     3061 acgggtgttg tgaaaaatgt cactgtcagc cagtcctgcg gtaagtggta catcagtatt
     3121 cagactgaaa atgaagtatc aactccggtt cacccttcag cattaatggt cggactggat
     3181 gctggcgtgg ctaaactcgc cacgctgtca gatggcacag tctttgggcc tgtaaacagt
     3241 tttcagaaaa accagaagac gctggcgaga cttcagcgac agttaagccg caaggtcaaa
     3301 ttcagcaaca actggcagaa gcagaaacgc aaaatacagc gactgcattc ctgtatcgca
     3361 aatatccgca gggactacct tcacaaagtc acaacgaccg tcagcaaaaa ccacgcaatg
     3421 atagtcattg aggatttgaa ggtcagcaac atgtcaaagt cagcagcggg tacggtcagc
     3481 cagccggggc gcaatgtccg ggcaaaatca ggtttaaacc gttcgatact ggatcagggc
     3541 tggtatgaaa tgcgccgcca gcttgagtac aagcagctct ggcgtggcgg tcaggtgctt
     3601 gctgttccgc cagcgtacac aagccagcgt tgcgcgtgct gtggtcatac agcgaaagaa
     3661 aatcgcctgt cacaaagtaa attcagatgc caggcatgtg gatatacagc gaacgctgat
     3721 gtaaatggcg ctcgtaacat tttagcggcg gggcacgccg ttcttgcctg tggagagatg
     3781 gtgcagtcag gccgcccgtt gaagcaggaa cccaccgaaa tgattcaggc gacagcctga
     3841 acgtagcagg gatccacgtc ct
//
LOCUS       DAZGBC010000278         3858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGBC010000278 DAZGBC010000000
VERSION     DAZGBC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.280"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..241
                     /locus_tag="ACHK1J_004920"
     CDS             <1..241
                     /locus_tag="ACHK1J_004920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914058.1"
                     /translation="ILWRISRRFSSVVTTSFRAAGTGDTGQAVEAACQPARKPLAGWR
                     LPEGMRATLARRPLPFPFVMCLTQECFFLMLWNCS"
     gene            436..687
                     /locus_tag="ACHK1J_004921"
     CDS             436..687
                     /locus_tag="ACHK1J_004921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914059.1"
                     /translation="MRENPWLAATGTVAPPLSRRGLRSWHPGKKTPGCKEQRRFANRA
                     SRRCGGSLLIACRVPHTRMSPCRRGLIQPQPAALTVQGK"
     gene            1111..1341
                     /locus_tag="ACHK1J_004922"
     CDS             1111..1341
                     /locus_tag="ACHK1J_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914060.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            1393..2754
                     /locus_tag="ACHK1J_004923"
     CDS             1393..2754
                     /locus_tag="ACHK1J_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO7914061.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPEQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDRYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2801..3364
                     /locus_tag="ACHK1J_004924"
     CDS             2801..3364
                     /locus_tag="ACHK1J_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO7914062.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            3364..3606
                     /locus_tag="ACHK1J_004925"
     CDS             3364..3606
                     /locus_tag="ACHK1J_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914063.1"
                     /translation="MTQGWREDKCPSVRAGKKPRAGGAERGRAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLLLLLLLLLFFFLPL"
BASE COUNT          809 a         1109 c         1147 g          793 t
ORIGIN      
        1 gattttgtgg cgaatcagca ggcggttttc ctcggtggtc actacatcct tcagggcagc
       61 aggcacaggt gatacagggc aggcggtgga ggccgcctgt cagcccgcgc gaaaacccct
      121 ggcaggctgg cgcctgccgg aagggatgcg ggccacgctg gcgcgtcgcc cgttaccctt
      181 cccgtttgtg atgtgtctga ctcaggagtg tttctttctt atgttatgga actgttcctg
      241 atgttgttgt tgttcttttc tctgctgctc tgattctgac tactgcagtg ttgtcctgtc
      301 tgccgggcgc accttcggct cccggctgcc tggcggcatc cggtcgtcag gcccgactcc
      361 gacaggcagc tgctggcgca cccgccagtc tccgccgaac ccgctggcgc ggtttcgggc
      421 tgtcgctacg gtccgatgcg tgaaaacccc tggcttgcag cgaccggaac ggtcgcgccg
      481 cccttgtcgc gccgtggcct gcgctcctgg catcccggca aaaagacgcc gggatgtaag
      541 gagcaacgcc gtttcgcaaa ccgcgcttca cgtcgttgcg gcgggtccct gctcattgcc
      601 tgccgggtgc cacacacccg tatgagtccc tgtcgccggg ggctgataca gccacaacct
      661 gccgcactga ctgtccaggg taaatgaaca gcccacaaaa ttttttaccc agaacccggt
      721 aaaaaatttt gcgttctgcc caggacaggt gcgtcaggcc gtggcagtga tgccccttgc
      781 ggcgaaggtt ctcaaacggg tgaagttcat accacaggga atacagcagc aggatcccgc
      841 gaaacgtcaa aaccgcggct cccgaaactc aacgggtgat gttctttaaa aatcaggatt
      901 tatttcaggc tccggcaata cgcatttatg tgttctgtca gcatcctgct ggcaggtaat
      961 gagaacgcag gaacagaagg aggaatacac cggaaaacgt cagatgcaga tatgaaaaaa
     1021 gcaggtcagg gacctgctta atccacgccg aagagactgt gcaaccagtc tttaaggcac
     1081 tgttaccaac ctgtcataag gtatccattt atgactgtaa gcattgtttc accttccgca
     1141 gcggctgtaa agccccgccg tcatccccgt ttcagacgcg aagacatccc cgcgccggaa
     1201 attgaccccg tgctgaaagc cttcgggcga cacatagccc ggagttttca ccgggggcgt
     1261 ggtgtacaca tccccgccat gaaaaacacg gctttcgggc aggtgctccg cacactcgaa
     1321 ctgaaacgcg cttttaactg atgaggtgag ccgccccgcg aggggcggca acagacggaa
     1381 ggagaacatt ttatgacatt agcagcaatc accatgaccg ccccggaagc cgccagccct
     1441 gtgcagatgt accgcgcgac ctactcaccg gatgacaaca aactgcgcct gtatgccgcg
     1501 tcacgtcttg acccggagac gtataaaaaa gtacatgatg ccggtttccg ctgggcacca
     1561 gaacaggcgc tgtttgtcgc gccagcctgg acaccgggcc gggaagacgt gcttctctca
     1621 cttgccggag agattgagga tgaagacagt acgctcgctg aacgtcagga agcacgggcg
     1681 gagcggttta ccggatacag cggaaagcgg gccagtgaat ccgcacaggc acttgatgaa
     1741 gtggaaagac tggccgcgat gatcccgccc ggtcagccca ttcttgtggg gcatcacagc
     1801 gaacgccgcg cccgtcgtga tgcacagcgt attgaaaacg gtatgaaacg tgctgtgatg
     1861 ctctttgaac gtgcggaata ctgggaagag cgggcgcggt cggcactgct tcacgcgaag
     1921 tataaagaac gtccggacgt tcgctggcgt cgtatcaaaa aaatcgaagc tgatttgcgc
     1981 aaggcggaaa agaccatcgc acagtcgcag aaatatctga cgatgtggcg ggctgaatcg
     2041 ctggatctga atatggcaaa actcatcagc agtcatgacc atatcagcgc ctgtttcccg
     2101 ctggacaggt atccgcgccc ggcagaaaaa agccagtatg aaggaagtcg atcgttatgg
     2161 tcggccctgg atgatgacat catcaccacg gagcaggccc gcgaaattgc gatccgctgt
     2221 catgaacggc agattcagcg tcagcaacgc tgggttaacc actatcagaa ccgcctgatc
     2281 tatgagcgtg ccatgctgga cgaaagcggc ggcgtggtta cccggacaca ggattttgag
     2341 ccgggcgggc aggttttcag ccggggcgag tggctgacca tcatccgcgt gaacaaaagc
     2401 aacggggcgg tgagttcagt cacaacgccg aattacagtt ttctcgggta cagcggcacg
     2461 atgaaagtga cgcccgatcg catcacggac tacaaagcac catcggcaga agaggctgcc
     2521 gtcgccagtc aggccgcgaa gcgtccgccg gtagtcaact atccggggga aggtttccgg
     2581 gaaatgacaa aggcgcagtg ggccgccctg ccccgggact gtaaggccgt gcgcagtgtg
     2641 gcagagacag aagaccacgg ggcataccgc taccgccgca caatggacaa taatttccgc
     2701 ctggtgaatg tgtatatcac cgacatgaaa atcacggaaa tcccacagaa ataaggtata
     2761 tccccccggg aaatccccgg gggatgacgg agataatgac atgcacagtc agttaagaga
     2821 acgtatccgg ctgatgcgcg caaggctgga taacgccgcg ccggttgctg aaatccgggc
     2881 tgaatctcag ctttttgtga ctcctgcccc ggtctgtgat cgcctggtga cgctggcgga
     2941 aatcagcaac cgtgatcaca ttctggaacc ctctgccggc accggggcca tactgcgggc
     3001 gattcgggat accgcgccgg aggccatgtg tgatgcggtg gagattaaca gcgggctggt
     3061 ccggtatctg cgggaaaatt ttaacggtgt cagggtacag tgtggcgact tcatggaatg
     3121 gctgccggtg cagtattaca gccggatcat tatgaatccg ccgttcagtc acgggcagga
     3181 tatccggcat atcctgcggg ctttttccct gttgcgtccg gggggcgttc tggttgccgt
     3241 ctgtctcaac gggccacgcc agcaggagaa gctgttaccg ttttctgacg tccgcgagga
     3301 gctgccgcgc ggcacgtttg cttataccga tgtcccgacg atgattattc gtttgcgtgc
     3361 ctgatgacac agggctggcg ggaggataaa tgcccgtctg tccgcgccgg gaaaaaaccg
     3421 cgcgccggtg gcgctgaaag gggccgcgct cacgcgccgc ccctttcatt acgcggacgt
     3481 tctgatgggg gcttctgtct tttcagacct gtcatttgtc tgttactgat gatgttgttg
     3541 ttcctgctac tgctactgct actgctactg ctactgctac tgctcttctt tttcctgccg
     3601 ctctgattct gactactgca gtgttgtcct gtctgccggg cgccccttcg gctcccggct
     3661 gcctggcggc atccggtcgt caggcccgac tccgacaggc agctgctggc gcacctgcca
     3721 gtctccgccg aacccgctgg cgcggtttcg ggctgtcgct acggtccgat gcgtgaaaac
     3781 cccgggtttg cagcgaccgt tccggtcgcg ccgcctctgt cgcgccgtgg cctgcgctcc
     3841 tggcatcccg gcaaaagg
//
LOCUS       DAZGBC010000279         3837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGBC010000279 DAZGBC010000000
VERSION     DAZGBC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.281"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(141..434)
                     /gene="yicS"
                     /locus_tag="ACHK1J_004926"
     CDS             complement(141..434)
                     /gene="yicS"
                     /locus_tag="ACHK1J_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO7914064.1"
                     /translation="MKLTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAIERNNQNNYWEALGKVECPDM"
     gene            643..1461
                     /gene="nlpA"
                     /locus_tag="ACHK1J_004927"
     CDS             643..1461
                     /gene="nlpA"
                     /locus_tag="ACHK1J_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO7914065.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            complement(1465..2388)
                     /locus_tag="ACHK1J_004928"
     CDS             complement(1465..2388)
                     /locus_tag="ACHK1J_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO7914066.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPSALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(2499..3683)
                     /locus_tag="ACHK1J_004929"
     CDS             complement(2499..3683)
                     /locus_tag="ACHK1J_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO7914067.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARNSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNVPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1146 a          855 c          896 g          940 t
ORIGIN      
        1 tgaaaataat gtagagggaa atttatccac aatcctgatg aactggttca attttgattg
       61 gaatttgaaa aagcaggtga tttactgatg tgcgagctta agtcagcggc tgctggcctg
      121 agcgcggagg tgtgcgaaat tcacatatcc gggcattcta ccttacccag cgcttcccag
      181 tagttattct gattattacg ctctattgcc acaatagctt cgcggatatg ttgtttgtta
      241 ctctcatcag acaacattaa tttttcccac gcttcgtctg ataatgacgc ctgtggcgtg
      301 caaattttac gtaaatcttg caataccgtg gttttctctt ttgccgactg tagcgaactg
      361 aatggcgact cggcctgaga aatggctgga aaagtcagga atacggtaat catgagtagc
      421 atcgttagct tcatagcggc aaccttctgc aaatgtgcat cgaatgaagc ataagtgtag
      481 tttgcttttg ccgctctcat catgctgtac gaaaggtgtt tgcgtggtca gtaagaagtg
      541 ccagtttttt ataccttttt cttctattcg atagcttttc gttttttaaa tcgtagtgat
      601 cttgccatta tagtcagtta atgattaaaa aaaggataaa aaatgaaact gacaacacat
      661 catctacggg cgggggccgc attattgctg gcaggaattc tgctggcagg ttgcgaccag
      721 agcagcagcg atgcaaaaca cattaaagtt ggcgttataa atggcgcaga acaagatgtc
      781 gcggaagtcg ctaaaaaggt ggcaaaagag aaatatggcc tcgatgttga actggtaggt
      841 tttagcggtt cgttgctacc gaacgatgcg acaaatcatg gcgaacttga tgcgaacgtc
      901 ttccagcatc gaccctttct tgaacaagat aatcaggcgc atggttataa actggtagcg
      961 gtgggaaata cgtttgtctt ccctatggcg ggttattcca aaaaaatcaa aaccgtggcg
     1021 caaataaaag aaggcgcgac agtggcgatc cccaacgacc cgaccaacct tggtcgcgca
     1081 cttttactgt tgcaaaaaga gaagctaatt actctgaaag agggaaaagg gttattacct
     1141 accgcgctgg atataaccga taatcctcgt catttgcaga ttatggaact cgagggggcg
     1201 caactcccgc gcgtactgga tgatcccaaa gttgatgtag cgattatcag caccacttac
     1261 attcagcaga ccgggctttc tccggtgcac gacagcgtat ttattgaaga taaaaattcg
     1321 ccgtatgtga atattttggt ggcacgggaa gataataaga atgcagaaaa cgtgaaggaa
     1381 tttctgcaat cttatcaatc atccgaagtc gctaaagcgg cagaaaccat ctttaacggt
     1441 ggcgcggtgc ctggctggta attctcactc atcccgcgcc ggacgattta tttttctggc
     1501 gcggcgacgg gaatccattg aaatcaaaat taccgatgac agaatcagca gcgttcccag
     1561 ccagtccggt aatgtgaacg tgatccccag caacagcaaa gagagtagcg cgctacttaa
     1621 tggttctgca cagctcaaaa tgctggcttt tggaccgcca attaattgtg ctcctttcag
     1681 gtacaggcta aatgtcagag atgtaccaat gaccaccaaa taaaaaaacg ccagaatcaa
     1741 actgccgtta acgacaaagt ttgtcccttg tctggcataa aaaggcaaca gaatcagacc
     1801 gccaatcagc atactccagc cgacgactgg taacgtgcca tagcgggcaa tcagcgttga
     1861 gggataggtg gtatagaatg cagcagcaaa tgccgaggca atgccccaga acaacgcgga
     1921 aggagagatc gataatgacg tcggattacc gtgtgtcacc aataaaaaag ttccgaccag
     1981 cgatgtcaaa atagcgcaga aaaccagaat gcccgggcgg gatttacgca ccagtgagaa
     2041 ccaggcgacg ataatcgtcg gtgagaggaa ttgcagcacc gttgccgtgg cagcattcga
     2101 tttttcgatg gttagcaaaa aagtgagctg tacggttagc gcgccaacca cggaaaaaat
     2161 cagcagacta atggcatctt tatgattgtt aataatagaa aagattttat cgccatgaac
     2221 aaatgacagc gtcagcagaa tcaaaccggc gaatatcaaa cgcgtcatag tcaaaaactg
     2281 cgatgacatc tggctttgct ccatgatgta ttgcgcacag acccctgaac ttccccacaa
     2341 cacggcggca atcagaacgt tcagcatccc ctttctggtg gaacccattt ttccctctgc
     2401 atactgtttt gtcgtttatg tatgcaggca tgatagcaaa aatggcgagg atggctctgt
     2461 ttgcgccacg ccgatttttc ttagcccaga ctggtgagct aaatatcttt aataaacagc
     2521 aagcaaatca tcgcaatacc caacatccct atcgccagcc agaacaacgc atggtagctc
     2581 cagatttcaa ccataattcc gtcaacagag ccggcgataa tccagccgac gcgtgaagta
     2641 tttgcatata acgttgtcgc agagcctatt ttttcaggca tcaggtcctg aaaataaagc
     2701 atgccgatac cacagagaat accaaggaag atggcattta gaatttgcaa ttccagctca
     2761 actgccggag tcgtcgccat gagtacgctg gcgtaaaaac acattccact cacgatagca
     2821 atgagcatta atagtcgctt gccaatacgt ttcatgtaat agcctgcgat taacatcatc
     2881 ggaatttcca gaccggcagc gataccaatc atttctccag ccagtttatc ggttagatgc
     2941 agttcatcaa taataaatag cggcatattt atcatgtaga gattattcgc cgcccacatc
     3001 attgaacaga ccacaaaaag tagccgcgta tctcgctttc tatggatgga gggtaaaatt
     3061 tctacgggtt gggtaacgac aggaacattt ctttgtattg atggcaaaaa caaccagact
     3121 atcagcccgc aaacaacaaa tgcgatggca gcggtgagat acatcacttt aaaactaaat
     3181 cccattgcca gttcataagc gagcggtggc ccgataaccc aggcaagcga gatctgagca
     3241 cgtaaaaatg tactgaacat gaccgtctca cggcctgttc tatcggcgtg ttcacgggcg
     3301 agggcgaaca tttgcgggtt tgcggtggaa gcaaaactac tcagaagtac gcccgttgag
     3361 agcagaatga agtagttgcg attccacgca aaaagcgtgc aggccagcac tccaaataag
     3421 caacatagca ggatcagtaa tttacggtcg ccttgtttat cggagttcct tgccagaaat
     3481 tgactgacca gaattcccat aatagcgcta ccggtgaaga aaaaacctac cattatagga
     3541 cgggctttca gttcatctgc gaggaatata cttagggtag gagtctgaag agcgcccgca
     3601 atacccgtca gaaaggcgac aagtaaaaat gccgcggcag tgagatcaag tatttttgat
     3661 ggagtggtag ccgttttttg cattcttctg aatcaccggg cgacaaaata taactgagtt
     3721 acaaataagt accagtcgaa tgagtattgt attaagaatt tttgagtatc gctgatacaa
     3781 ccagaaaaca atgctagcgc aatattatag ggccagcaat tgagattatt agcaaaa
//
LOCUS       DAZGBC010000280         3807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGBC010000280 DAZGBC010000000
VERSION     DAZGBC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.282"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..2360
                     /gene="ydeP"
                     /locus_tag="ACHK1J_004930"
     CDS             81..2360
                     /gene="ydeP"
                     /locus_tag="ACHK1J_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO7914068.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICSGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2608..2805
                     /gene="safA"
                     /locus_tag="ACHK1J_004931"
     CDS             2608..2805
                     /gene="safA"
                     /locus_tag="ACHK1J_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO7914069.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2879..3640
                     /gene="ydeO"
                     /locus_tag="ACHK1J_004932"
     CDS             2879..3640
                     /gene="ydeO"
                     /locus_tag="ACHK1J_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO7914070.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT         1069 a          839 c          875 g         1024 t
ORIGIN      
        1 ttggcgataa tccctttgta tgtactgtgc atcatcgcta ttacaaatcc taataattca
       61 tttccacaca ggataagtag atgaagaaaa aaattgaatc ctaccaggga gctgccggtg
      121 gttggggtgc tgttaaatcc gtagcgaatg cagtacgtaa gcagatggat atacgccagg
      181 atgttattgc catgtttgac atgaataagc cagagggctt tgactgtccg ggttgtgcat
      241 ggccagatcc taagcacagt gcgtcattcg acatttgtga aaacggcgca aaagcaatcg
      301 cctgggaagt cacggataag caggtaaacg cctctttctt tgctgagaat acggttcaat
      361 cattacttac ctggggagac cacgagcttg aggctgcggg gcgactcact cagcctttga
      421 aatatgatgc cgtcagcgac tgttacaagc cattaagctg gcaacaagct ttcgatgaaa
      481 ttggcgcacg ccttcaaagc tatagtgatc ccaatcaggt tgaattctat acttcgggcc
      541 gcacttccaa tgaggctgcc tttctttatc agctttttgc ccgtgaatac gggagcaata
      601 actttcccga ctgctccaac atgtgccatg aaccgacaag cgtgggtttg gcagcgagta
      661 tcggtgtagg taaagggacc gtgttgctgg aagactttga gaagtgcgat ttagtcattt
      721 gcattgggca taaccctggt acaaaccacc ctcgcatgct gacttcgttg cgcgctttag
      781 tgaaacgggg agcgaaaatg atcgccatca atcctctaca ggaacgtggc ctggagcgat
      841 ttaccgcacc gcaaaacccg tttgaaatgc tgacgaactc tgagactcag ttggccagtg
      901 cctactacaa cgtgcgcatt ggtggcgata tggcgttgct caaggggatg atgcgcctgt
      961 taattgagcg cgatgatgct gcaagtgccg caggtcggcc ctcattgctg gatgacgaat
     1021 ttattcaaac gcataccgtc ggctttgacg agctacgccg tgacgttctc aattccgagt
     1081 ggaaagatat cgaacgtatt tctggactaa gtcagacaca aatcgccgaa ctggctgatg
     1141 catatgccgc tgccgaacgc accattatct gttacggaat ggggatcact cagcacgaac
     1201 atggtaccca gaatgtacag caactggtca atctgctgtt gatgaaaggt aacattggca
     1261 agcctggtgc gggtatctgc ccactacgtg gccactctaa tgtacagggc gaccgaaccg
     1321 tcggtatcac cgagaaaccg tctgcagagt ttctggctcg cctgggtgag cgctatggct
     1381 tcacaccacc tcaagcacct ggacatgctg caattgccag catgcaagca atatgttcag
     1441 ggcaggctcg agcattgatc tgcatggggg gcaactttgc actggcaatg ccagatcggg
     1501 aagcgagcgc tgtaccgtta acgcaattag atctggcggt acacgtagcc actaagctta
     1561 accgctctca tctgctgacc gcacggcata gctatattct gccggttctg ggacgtagcg
     1621 agattgacat gcaaaaaagc ggtgcgcagg cggtaaccgt tgaggattca atgtcgatga
     1681 ttcatgcctc gcgtggcgtg ttaaaacccg ccggtgtaat gctgaaatca gagtgtgcag
     1741 tggtcgcggg aatcgcgcag gcagcactac cccagagcgt ggtagcctgg gagtatctgg
     1801 tggaagatta tgatcgcatt cgcaatgaca ttgaagctgt gctgccagag ttcgccgatt
     1861 ataaccagcg catccgacat cctggtggtt ttcacctgat aaacgctgct gctgaaaggc
     1921 gctggatgac tcagtcaggt aaggctaatt tcattaccag caaagggctg ttagaagatc
     1981 cctcttcagc gtttaacagt aagctggtca tggcgacagt acgcagccac gatcagtaca
     2041 acacgacgat ttatggtatg gatgatcgct atcgaggggt attcggtcaa cgagatgtgg
     2101 tctttatgag tgctaaacaa gctaaaattt gccgtgtaaa aaacggcgaa agagttaatc
     2161 ttattgcgct tacgccagac ggtaagcgca gctcacgccg catggataga ttaaaagtgg
     2221 tcatttaccc tatggctgac cgctcactgg tgacctattt tccagaatcg aatcacatgc
     2281 taacacttga taaccacgat ccattaagtg gcattcctgg ctataaaagt attccggttg
     2341 aattagaacc atcaaattaa tgcctcttct catttcttct gctgtcatcc gcacagcaga
     2401 agaatacctc attgactatt atcgcgcaat ttgctcacat ggattaaatt aaactacata
     2461 ctataagata taaacttctg cctacagctg taagaaactc cgctcagtac tgaagtacca
     2521 gtcctatttc ctcttttctc aagcctgtta tattaagcat actgattaac gattttcaac
     2581 gttatccgct aaataaacat atttgaaatg catgcgacca cagtgaaaaa caaaatcacg
     2641 caaagagaca actataaaga aatcatgtct gtaattgtgg ttgtcttatt actgaccctt
     2701 acgttgatag ccattttttc ggcaattgat cagctgggta tttcagaaat gggtcgcatt
     2761 gcaagagatc ttacacattt cattatcaat agtttgcaag actgaaaata aacagcgaat
     2821 tataaatatg aaatgttaaa aaagtatcga taaaacttta ttgttttaag gagataaaat
     2881 gtcgctcgtt tgttctgtta tatttattca tcatgctttc aacgctaaca ttttagataa
     2941 agattacgcc ttctctgacg gcgagatcct gatggtagat aacgctgttc gtacacattt
     3001 tgaaccttat gagcggcatt ttaaagaaat cggatttaat gaaaatacca ttaaaaaata
     3061 tctacaatgc actaacatcc agacagtgac ggtgcctgtt cctgcgaagt ttttacgtgc
     3121 ttcaaatgta ccgactggat tgcttaatga aatgattgct tatctcaact cggaagaacg
     3181 caatcatcat aatttttcag aacttttgct tttttcttgc ctgtctattt ttgccgcatg
     3241 caaaggtttc attacactat taactaacgg tgtgctatcc gtttccggga aagtgagaaa
     3301 tattgtcaac atgaagctgg cgcacccatg gaagctgaaa gatatttgtg actgcctgta
     3361 catcagtgaa agcctgttga agaaaaaact aaagcaagag caaacgacat tctcacagat
     3421 tctattagat gccagaatgc agcacgcaaa aaatttgata cgcgtagaag gttcagtcaa
     3481 taaaattgcc gaacaatgtg gttatgccag tacatcttat tttatttatg cgttccgcaa
     3541 acatttcggc aacagtccga agagagtttc taaggagtac cgttgtcaaa ggcacaccgg
     3601 tatgaacacg gacaacacga tgagtgcttt agctatttga ttatttgcta acgggtagtc
     3661 aaccacacac gctacgtaag aattaaatgg ggcagccatt ccttgccccg cgttgttttt
     3721 aggcgatata tttattgaaa taaataagtg acattcatca catatttatg catttgcata
     3781 acctgttgca tgattattta tgatctc
//
LOCUS       DAZGBC010000281         3806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGBC010000281 DAZGBC010000000
VERSION     DAZGBC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.283"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            133..1479
                     /gene="yagA"
                     /locus_tag="ACHK1J_004933"
     CDS             133..1479
                     /gene="yagA"
                     /locus_tag="ACHK1J_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFO7914071.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTRQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            1758..3638
                     /locus_tag="ACHK1J_004934"
     CDS             1758..3638
                     /locus_tag="ACHK1J_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFO7914072.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     SIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWTFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(3656..>3806)
                     /locus_tag="ACHK1J_004935"
     CDS             complement(3656..>3806)
                     /locus_tag="ACHK1J_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HFO7914073.1"
                     /translation="GKELWQKDFFTNPVGGSLTAIFELFCFVISVPVVAIYLIFIFCK
                     ALSGK"
BASE COUNT         1116 a          797 c          924 g          969 t
ORIGIN      
        1 aaaaacaatt catttgaaag gatatcagaa gaagatatcg gggcgcacag ttcatctctg
       61 cctttgtttc atggggtgtt ctttgtatat tttacttact gtttaagcat gtacataagg
      121 agtgctttca ttatgccaat gatacttgta aagaagaatc cccggggcaa agtgattcgg
      181 gagcttagca gtgaggaaga gactgcagta aagacggtct gtggactgaa aaagccagca
      241 accatggcac tacataacct ggccaatgat attctccggg aaatgcgtga gtatgatgca
      301 tggttacagt gtgactgtat tccgggagat tcgccggcca tgaattttgc tgctcttaaa
      361 aacaatacgg gtactcttta tctgtccagt tttaatcatg agcatgcacc tgaatgcccc
      421 atgtatcgtc agttatccgg aaatgaagag gaaacatctt ccggagcaag ccggcatccc
      481 gttagcaccc gtataaatta cagaaatttt ttaccacctg atgacagtaa ttctgttatc
      541 agactgcagg cccgctctgc ttaccataat ggtgagagct cctccgttcg taagaaacgt
      601 ccgcgccttg cccgtttact cctgtctctt attgacgatg ctgggctgaa taaactggat
      661 tccctggcta atccccgtat ccggactaat tatcgtgagt gtcttgatgc catacggcag
      721 gttacacggc agcaggagta tattcgggga agagccttat ctgaaattat ccatttccgg
      781 cctggaatgt ctgaaaggtc tcaggagcgg ttaatggaaa cgctggagaa ttcagaacga
      841 cactggcctg ccagaagaaa acatatgttc tttcagattt ttatggcgca gcacatctgt
      901 cgtgatgctg tggaaattca ctgggcgaat ggtaatattc aggtcatcag acctgtgcgg
      961 gggatcagta ttaatggtga agcgcagggc ggaatacgtc ccccttactg ggttattctt
     1021 gctttttgcc ggagtgccga tggcagaatc atctgcagtg aagggtatgc tcatgctctt
     1081 tatcaactga catgtccggt gcctgtggac agcaaactgg aacgaaacac gctcactgct
     1141 cttctgaatg tggccagctg gcttaaaaga aagccaggta caccggaatt aagtctggaa
     1201 aggcccctgt ttgatacaga agtttatgtt aatggtgaaa agaaatatgt cctgccggat
     1261 ttcattgtca cagcaagggc tcctgacgga aagacggcca gagtggtcat cgaaacgatg
     1321 ggatatgaag acagtgatta ctgcgcgaga aaatccaggc agcataccgg catgaagcag
     1381 attggtgttc tgcataccga tccaccgaaa tggctggata acgatcatcc cccttttgag
     1441 aaacatatgt acggtgtttt tatgcatctc aggtactgag atattttgtg gctcagttct
     1501 gtaacttttc ccgtaacatt gtctgttgtt acgggaaagt ccggtttttg tattgcacca
     1561 gagaatactc agattgtgat gctgccacag cgtcagcagg ctttctgaac ggtgtaacat
     1621 cacttcatgt taatgataat cactatcatt aaatcttgac atgccatttt ctccttaata
     1681 aattaatgct gtatatgtat ccatatacgt aagcagttaa ttcatttgtt ttcctcagag
     1741 gatgaaggag ataccgaatg tctgaccctg tacgtattac aaatcccggt gcagaatcgc
     1801 tggggtatga ttcagatggc catgaaatta tggccgttga tatttatgta aaccctccac
     1861 gtgtcgatgt ctttcatggt accccgcctg catggagttc cttcgggaac aaaaccatct
     1921 ggggcggaaa cgagtgggtc gatgattccc caacccgaag tgatatcgaa aaaagggaca
     1981 aggaaatcac agcgtacaaa aacacgctca gcgcgcagca gaaagaaaat gagaataagc
     2041 gcactgaagc cggaaaacgc ctctctgcgg cgattgctgc aagggaaaaa gatgaaaaca
     2101 cactgaaaac actccgtgcc ggaaacgcag atgccgctga tattacacga caggagttca
     2161 gactcctgca ggcagagctg agagaatacg gattccgtac tgaaatcgcc ggatatgacg
     2221 ccctccggct gcatacagag agccggatgc tgtttgctga tgctgattct cttcgtatat
     2281 ctccccggga ggccaggtcg ttaatcgaac aggctgaaaa acggcagaag gatgcgcaga
     2341 acgcagacaa gaaggccgct gatatgcttg ctgaatacga gcgcagaaaa ggtattctgg
     2401 acacgcggtt gtcagagctg gaaaaaaatg gcggggcagc ccttgccgtt cttgatgcac
     2461 aacaggcccg tctgctcggg cagcagacac ggaatgacag ggccatttca gaggcccgga
     2521 ataagctcag ttcggtgacg gaatcgctta acacggcccg taatgcatta accagagctg
     2581 aacaacagct gacgcaacag aaaaacacac ctgacggcaa aacgatagtt tcccctgaaa
     2641 aattcccggg gcgttcatca acaaatcatt ctattgttgt gagcggtgat ccgagatttg
     2701 ccggtacgat aaaaatcaca accagcgcag tcatcgataa ccgtgcaaac ctgaattatc
     2761 ttctgaccca ttccggtctg gactataaac gcaatattct gaatgaccgg aatccggtgg
     2821 tgacagagga tgtggaaggt gacaagaaaa tttataatgc tgaagttgct gaatgggata
     2881 agttacggca acgattgctt gatgccagaa ataaaatcac ctctgctgaa tctgcggtaa
     2941 attcggcgag aaataacgtc agtgccagaa caaatgaaca aaagcatgca aatgacgctc
     3001 ttaatgccct gttgaaggaa aaagagaata tccgtagcca gcttgctgac atcaatcaga
     3061 aaatagcgga agagaaaaga aaaagggatg aaataaatat ggtaaaggat gccataaaac
     3121 tcacctctga tttctacaga acgatatatg atgagttcgg taaacaagca tccgaacttg
     3181 ctaaggagct ggcttctgta tctcaaggga aacagattaa gagtgtggat gatgcactga
     3241 acgcttttga taaattccgt aataatctga acaagaaata tagcatacaa gatcgcatgg
     3301 ccatttctaa agccctggaa gctattaatc aggtccatat ggcggagaat tttaagctgt
     3361 tcagtaaggc atttggtttt accggaaaag ttattgatcg ttatgatgtt gctgtggagt
     3421 tacaaaaggc tgtaaaaacg gacaactggc gtccattttt tgtaaaactt gaatcactgg
     3481 cagcaggaag agctgcttca gcagttacag catggacgtt ttccgtcatg ctgggaaccc
     3541 ctgtaggtat tctgggtttt gcaattatta tggcggctgt gagtgcgctt gttaatgata
     3601 agtttattga gcaggtcaat aaacttattg gtatctgaca cataatgatg ccctttcatt
     3661 ttcctgaaag ggctttgcag aatataaaaa ttaagtaaat ggcaaccaca ggaactgata
     3721 taacgaaaca gaataattca aatattgcag ttaagcttcc gccaactgga tttgtaaaga
     3781 agtctttttg ccagagttct ttccct
//
LOCUS       DAZGBC010000282         3798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGBC010000282 DAZGBC010000000
VERSION     DAZGBC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.284"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..93)
                     /locus_tag="ACHK1J_004936"
     CDS             complement(<1..93)
                     /locus_tag="ACHK1J_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914074.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTP"
     gene            508..1467
                     /locus_tag="ACHK1J_004937"
     CDS             508..1467
                     /locus_tag="ACHK1J_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO7914075.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDSEVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            1467..1862
                     /locus_tag="ACHK1J_004938"
     CDS             1467..1862
                     /locus_tag="ACHK1J_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7914076.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDSLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDINVLKNIIE
                     ASSENKSESIASKDDFEEQTRRNLSMLKK"
     gene            complement(1977..2309)
                     /gene="pemK-mt"
                     /locus_tag="ACHK1J_004939"
     CDS             complement(1977..2309)
                     /gene="pemK-mt"
                     /locus_tag="ACHK1J_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFO7914077.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(2311..2568)
                     /gene="pemI"
                     /locus_tag="ACHK1J_004940"
     CDS             complement(2311..2568)
                     /gene="pemI"
                     /locus_tag="ACHK1J_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFO7914078.1"
                     /translation="MHTTRLKKVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPHRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2661..3314)
                     /locus_tag="ACHK1J_004941"
     CDS             complement(2661..3314)
                     /locus_tag="ACHK1J_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFO7914079.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGPGKNYLF
                     FSHGAVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYIGVFLLGKVLAL
                     PAEPFMTKLFADKSIPDVSLTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYKNLLTLAELFLVGGITSAARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT         1193 a          935 c          789 g          881 t
ORIGIN      
        1 cggtgtgact gcaccgctgg ccagtcgttt aatctcagcg atctgtgccg gttcaccaga
       61 aaaatacata cgattcgaac accagtttgg catgggaatt ctcctctcac atgcggcctc
      121 catcaggccg tgccgcacct gtaaccgggc gaccgcatac gaatgcaggt gcggccccga
      181 agccgcagcg cgtggaggag gaacgacgaa acgtgaccgg gtgaggaggc cggcgacagc
      241 gacacacttg catcggatgc agcccggtta acgtgccggc acggcctggt taagtagttt
      301 tcgtctcagt ttgtgacgca aaatgagaaa ccaataacaa ctcaaattga gtcacagcaa
      361 gaacacaagg agactctaaa agagccacgc tacttgacat taacccaaaa tgagacacaa
      421 taagaaataa caaaaactca aaaagatgca tcgacataaa agatgctcaa tttgagacaa
      481 acacaaactc aaaacgaggc aaaaagaatg ctaaaagtat catgcgatga tggttcaact
      541 aacgtaaaac tagcctggct tgaagatggc gaggtaagaa ccagtctgtc aggaaacagt
      601 tttaaagaag gctggaaccc gggactgttt aatgccggca aagtttacaa ttacgtcgtg
      661 gatggaaaaa aatacaccta tgatttgggc agcactgctg tcataggtac aacacatgtc
      721 agttatcaat acagcaccac caatctgctt gccatacatc atgcactgct gacatccggc
      781 cttcagccac aggatgtaga actgacagtg acattacctg taaccgaatt ctttgataat
      841 gacaatcaac caaatgaaga aagaatagaa agaaaaaagg caaatgttct ccgtgagata
      901 tctctgaata aaggggaaac atttaaaatt aaaaaagtta atgtaatgcc tgaatctttg
      961 ccagctgcat ttgaatcact aaaaaaagat aaagtaaaca aacttgaacg cagcctgatt
     1021 atcgatcttg gaggaaccac tctggattgt ggtttaattc tcggtgcatt tgaaggcatt
     1081 tctgaaatcc gtggctattc agaaatcggg acatcccgca taactcatac cgtcatgaat
     1141 gcactaacaa aagcctccac accctgcaat tattttattg ctgatgagtt aataaaaaac
     1201 aggcatgata atgaatatct ccagacatta ataaatgatg tagctgaaat aaaaaatatt
     1261 actcatgtaa tagacagtga ggtgaaatct ctggcagaaa gcatcaggca ggaaatttca
     1321 acattctcag gaatgaaccg aatctacctc acaggcgggg gagctgagct tatttaccct
     1381 cacataaagc aatatttccc gaatctgaaa gtgaacaagg tagatgaacc tcagtttgca
     1441 ttggtgaaag caatggtgca cgcgtaatgg aaagcagtga tccgaaaaaa agaaagaaag
     1501 ttgtggcata cctgcacccg acactttacc cgcaggacag cctgacacaa caaacaatag
     1561 actctctccc gatacaaatg cgaggagact tctacagaca atctcttatt tgcggagcag
     1621 ctctttattc tgttgacccc cgactgttaa cgctaatcag tggttttttc agtgagaaaa
     1681 tcactgcaga aaatctggta aaacttatag agcaaacaac aggatacaca tccacatcaa
     1741 ttgatataaa tgtgttaaaa aacattatag aggcatcttc agaaaacaaa tcagagagca
     1801 tcgcttcaaa agatgatttc gaagaacaaa ccagacgcaa tctgtctatg ttaaaaaaat
     1861 aataaaagaa gagcaggttc agttattacc tgctcttctt ttataatgag ggaccagcag
     1921 cggttttagt gccctaaatc gaacgttttc gtccggttgc ccctcaaacc ccatgttcaa
     1981 gttagaatag tggacagacg gccaagaact tcgttcatga tagtctctgg aacccgttcg
     2041 agtcgttttc cgccccgggc tttcatatca attgtccggg gttgatcgca acgcacaaca
     2101 cctgtggtac gtatgccaac accatccagc gacaccgcaa agccggcagt gcgggcaaaa
     2161 ttgcctccgc tggttacggg cacaacaaca ggcagtcggg tcacgcgatt aaaggccgcc
     2221 ggtgtgacaa tcagcaccgg ccgcgttccc tgctgctcat gacctgcggt aggatcaagc
     2281 gagacaagcc agatttcccc tctttccatg tcagatttcc tcctgaccag tcgccggtgc
     2341 atccagccat tctcgttctt cagctgatat ttcagcattc ggatcacact gtgccagtag
     2401 ctcagccagt gaatattgcg ggcgtctgtg cggctcaaca atcagccggc cattatcaat
     2461 gaccatgcca acttcattat ctgtacccag agacagcgca ttcagcagtg ccggtggaac
     2521 ggtcagcata actgagccgc caaccttctt cagtcgggtg gtatgcattc ttcacctcca
     2581 taaaagttat atttaaatat aacatccact aaaaaaacac accaggcttt aacgcgcaat
     2641 gtttaataaa aatataactt tcaaccaaac agtaaaccca gcgtggttgc ttccatatgc
     2701 agcaataccg gcagcagcag gccaccactt ctgatcctgg ccgctgatgt aatccccccc
     2761 accaggaaca gctctgccag tgtcagcagg ttcttatact ggctatgcgc ggcgacgaac
     2821 aacaacgacg ttatcagcgc ccccagccac atcgtccagc agtaccgtga acggaagacg
     2881 ttcagcataa tcccccggaa cagcgtttcc tcattcaacg gggcaaggat aaagatggtc
     2941 agcaacgtca ggctcacgtc aggtatagac ttatcggcaa aaagtttcgt cataaatggc
     3001 tcagcaggca gagccagcac cttaccgagc agaaatacac cgatatacac cacggccatc
     3061 gcaccgacca gccacggtac gccaacgttg cgcagctgac caacgaccgg tagcggagct
     3121 atccaacggc ggtataccag gaaaacacac agcaggtaca tcagaacagc accatgactg
     3181 aagaacaaat agttttttcc tggcccataa agcagaacgg cctgctccat gacaaatctg
     3241 gctccccagc taatgcccca tgcagccagc ataaccagca taaactgcag atattgatta
     3301 cgtgtctgaa tcattgtatc tcctgtaaat ttttaacttg tcctattttt gtcattacta
     3361 catatataca catgtataac aattcagata tcgtcaccag gatatgccgc gccagcggca
     3421 tggaaggcgg cactccgctg tttcatatga taccgccgaa gcccgatgta agccgctaca
     3481 gtcgtccgaa agtcaccagc ctcctccccc tgccgtcatc cgtgcatcag ctatgcactg
     3541 agtatgcctg cccttcccta gagaatcctg ccaggcttgc cacactgata tatcttgact
     3601 ttatgtaaac gatatgacac cttaacatga taatgattac cattctcttt taatatacag
     3661 agaaactagg aaatagatga atgagttatg ttactttaat attctctgac aataacctaa
     3721 atcagttaga ttattgtcat ttaataaata atgacattct ttcatcataa ataaaaagac
     3781 tattgtttat catattgt
//
LOCUS       DAZGBC010000283         3793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGBC010000283 DAZGBC010000000
VERSION     DAZGBC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.285"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..996
                     /gene="traW"
                     /locus_tag="ACHK1J_004942"
     CDS             <1..996
                     /gene="traW"
                     /locus_tag="ACHK1J_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO7914080.1"
                     /translation="LASVIAQDGQATRQQMIFSNETRRLEEARKSFSVPDSICSESAS
                     GVAAESRRAAASASARLSQGGGVSSKPIRERLSRAADSPTREAYDSAGIHAGYCTEAE
                     YARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQIDAATAYMKNTAR
                     PSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADSTPNEATRGALTEA
                     LQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQDLQGMEGDNLLRE
                     LLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLSELSQAMSQGSVR"
     gene            993..1562
                     /gene="traX"
                     /locus_tag="ACHK1J_004943"
     CDS             993..1562
                     /gene="traX"
                     /locus_tag="ACHK1J_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO7914081.1"
                     /translation="MRDGNSTDEKKTATRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            1635..>3793
                     /locus_tag="ACHK1J_004944"
     CDS             1635..>3793
                     /locus_tag="ACHK1J_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO7914082.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASNNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTATSASEYQASNTQNPQSALLKLLGNDLTLFTNQIATIHSGTGTT
                     SDQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGVGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIMAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINGDDKDGI"
BASE COUNT          893 a         1067 c         1035 g          798 t
ORIGIN      
        1 ctggcctctg tcatcgcaca ggatgggcag gccacccgtc agcagatgat attcagtaat
       61 gaaacccgcc ggcttgagga agcccggaag agcttcagcg tgccggactc catctgcagt
      121 gaatccgcct ccggagttgc agctgaaagt cgccgtgcag cagcgtctgc gtctgcacgc
      181 ctgagtcagg gaggaggcgt cagcagtaaa cccatccgtg aacgtctctc ccgggcagca
      241 gactcaccaa cccgcgaggc atatgacagt gcaggcattc atgccggtta ctgcacggaa
      301 gctgaatatg cccgctttgg cggaaccgat gtctgccctg ccgtgggcga gctgccgggc
      361 ggagacagcc aggtgcgatc gctctatcag ggtgcaggca cagctgatac ccctgccgcc
      421 ctcacctggg atcagaaaca gattgatgct gccacggctt acatgaaaaa caccgccaga
      481 ccgtcagcgg gtcgtgcccc ggggaaaggt gaagtgggaa cacagaccgg gcgcacgtat
      541 gtgggcctgc agaatgaata caacggcatc atcgatgccg cctctcatcc gcaactgtca
      601 ctgatagctg acagcacccc taatgaggcc acccgtggag cgctgactga agccctgcag
      661 tcaccatccg ctgcggcata ttttgaccgg accgcatcat cagaggcccg cacccgtggt
      721 cacatgagcc agcgggagtt tgaggccttt gaagctggca gacgctatgc aaacaccgac
      781 tggcagcagg atctgcaggg catggaaggc gataaccttc tccgtgaact gctgcgcact
      841 accgccctgc tgaactggca gatgaacgac ctgaaagagc agatacgcca gggtaatgtt
      901 atcgccgggc agcagctggc actggcggca cgacagtatt acggacagcg tctaagtgaa
      961 ctgtcacagg ccatgagcca ggggagcgta agatgagaga cggcaacagc accgacgaaa
     1021 aaaaaacggc cacccgtctc cgccgcatcg gtgacatcgg gataaacctg ttcgcaccac
     1081 tggcagaaac ccggtggatg ctgcgcagct gccgccatat gctgggccag cataaaacgc
     1141 gcctgaagcg cctcatgccc gaccggcaac cggagagtcc ggtctgtatg acctgggaag
     1201 aagccgtcag ggccagcggc cttacggtca acgaactgga tcggcgtctg cgacgtcgca
     1261 ggatgttctg gcggacagga ttctggctga tgatgatacc ggtacttctg ctgtgcggca
     1321 tggcccttgc tgccacagcg cttccggcca tcaccctgat acgtatactg gcctgcctgt
     1381 tactgatgag tgctattgca gcactgtgtg cctcaaaaac cctgattgtg tcataccgcc
     1441 tctggcaact gaatgaacgg cgggtcagcg aggacgaaca gggcaccttc cccgattacc
     1501 tgcaggcccg gcacggctgg aaacaggcca catggctggc cattacccac cggcagtact
     1561 gaccgtccgg tctgcaattc cctttcctca ttccggaggc tgatgtcctg catcccttcg
     1621 gttccggagt tatcgtgaaa atcataccca gagcactgag tgcaggtctg gcgctggctg
     1681 ccgtacctgc acaggcattc gtcacctatc aggacattgt gagcgccgcc accaatccgg
     1741 atgacctgtc gcgccaggca ctggtcacca tttttggcga cgtggtcaca aatcccctga
     1801 cagccagcaa caacaccatt atcggtaacc tgttcatggt gtttaacgca attatcgcca
     1861 ttatggggat agtctggttc ctgtttattg gaatccgcca tgttgtccgc accggccacc
     1921 agggcaatgt ctttaacaac ggacgggacg tcgtcggaat gctcagtatt gtgagcggtt
     1981 ttatgatgat tgtgcccacc gcttccggct ggagcctggc acaactggtc atgctctggg
     2041 gcgcatccat catgggcatt ggttccgcca atatcatggt tcagacggct gcagaaaaca
     2101 tcgctgaagg ctattcaatg accgtacagc cagttcatgt ctccacccgc acggcagcac
     2161 ggggaatact ggagatggcg ctgtgcaaac acgccatgaa tgcaggcctc gatgacttta
     2221 accgtactgc ccgttcatca acgcccccca tgagcgaatc ctcacggaca gagaacggca
     2281 ggtataccgt caccgtttca aatggcagtg ccacctgcgg gagtgtgagt ctcaccgtga
     2341 aagggaatgg cagcaacaac cagtccgcac tggactattt tttcaatccc ttcattgaaa
     2401 cggagtatca gggtgtcctg acggcacaac ggtctgccat ggataccatg ctccgtgacc
     2461 tggacagcgc agcacaggaa tttgtgtcag cgtaccggac ccgacgcgac acgggtaacg
     2521 gcaacctgcc ggatattgaa agccgtattc agaaggctgc caacaggtat gaagaaacgg
     2581 tgcagaaggc gttgccaaca gataacgcag aaactgagcg cagggagcag ctgaaatcct
     2641 acctgaccac ctacggctgg gttgccctgg gggcctggta ccagaccttt gccactgcca
     2701 accagcgact ggcagaactg gccgaccgtg cccccactgt gagtggagtt tcatcgcagg
     2761 gcgaagcagg ggatactgaa ttcctgaagg aagtaaaagg agcttaccag gcacagttgc
     2821 aaaactcacc atatacacct gcactgggaa cagccacctc agccagtgaa taccaggcat
     2881 ctaatacaca gaatcctcaa tcagccctgt taaaactcct agggaatgac ctgacgttat
     2941 ttaccaatca aattgcaacc atacattcag gaactggaac cacctcggac caactaaatc
     3001 cgctaataaa aatgaaaaac attggtgatt acaccatggt cacaaccgaa accatctggg
     3061 cgggttatac caccgtcagg gtactttcca ctgctggcag caactccatt tttggtcgac
     3121 tggtcaatgc tgttagcggc ttatctgacg ccgcaaaggc ttttctggaa gcatccgcac
     3181 caccgctcta cttcctgctg tttttgctgt tctgtgccgg tttttcgtta tccatttacc
     3241 tgccgtttat tccgtttatc ttctggatga ccggcgtcgg taactggata gtcagtgtac
     3301 tgattggatg cgccgccggc cccctgtggg ccgcaaccca tctgggaaca agccaggaca
     3361 ggggaagccg tgctgcttat ggttacatct acctgataga cagtatgatc agaccactgc
     3421 tgatggtgtt cgggttcttt tttgcatcgg tggccatcat ggccggcggc acaatactga
     3481 atgcgctgtt tgccgccgcg ctgatgaatg tccagataaa ttctgtgacg ggcgtgttca
     3541 gcatggcagg atttttgatg atttatgccc gcgtctgtac cacgctggtt gccgccgttt
     3601 ttgcacttca ggcttatctg cctgaccatg taattaattt tctgggcgga cgtgacggcg
     3661 ttaatatgct ggggaatatg gtttcttcag tcaaagatat tattgccgga ggtaatacca
     3721 atatgcgcag aacacctggc gttcgtgaag aaaaactgaa aaatattaat ggagatgata
     3781 aggacggaat aaa
//
LOCUS       DAZGBC010000284         3775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGBC010000284 DAZGBC010000000
VERSION     DAZGBC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.286"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..91
                     /locus_tag="ACHK1J_004945"
     CDS             <1..91
                     /locus_tag="ACHK1J_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7914083.1"
                     /translation="WRRQIDKRAAEKISASQSAGSKPEEPITP"
     gene            117..362
                     /locus_tag="ACHK1J_004946"
     CDS             117..362
                     /locus_tag="ACHK1J_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7914084.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGDPTPDSDMMM"
     gene            439..654
                     /locus_tag="ACHK1J_004947"
     CDS             439..654
                     /locus_tag="ACHK1J_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7914085.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            658..885
                     /locus_tag="ACHK1J_004948"
                     /pseudo
     CDS             658..885
                     /locus_tag="ACHK1J_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            1538..1825
                     /locus_tag="ACHK1J_004949"
     CDS             1538..1825
                     /locus_tag="ACHK1J_004949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914086.1"
                     /translation="MQPNTGAHSSTFGVPENMQSVLSNADELLLIHLTKEKRSESLVC
                     LNIQQNPQGVSRSVPGVYESHNIDRDDIYEWLFYPMVVLCVPVNGGLPLDR"
     gene            1960..2493
                     /locus_tag="ACHK1J_004950"
     CDS             1960..2493
                     /locus_tag="ACHK1J_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7914087.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            2767..3462
                     /locus_tag="ACHK1J_004951"
     CDS             2767..3462
                     /locus_tag="ACHK1J_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO7914088.1"
                     /translation="MKEMISVDREISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPQTLPEALRFAAKLAEQKAELENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            3557..3688
                     /locus_tag="ACHK1J_004952"
     CDS             3557..3688
                     /locus_tag="ACHK1J_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914089.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            3700..>3775
                     /locus_tag="ACHK1J_004953"
     CDS             3700..>3775
                     /locus_tag="ACHK1J_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7914090.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRD"
BASE COUNT         1015 a          798 c         1030 g          932 t
ORIGIN      
        1 ctggcgcagg cagattgata aaagggccgc agagaaaatc agcgccagtc agtcagccgg
       61 aagcaaaccc gaagagccca ttactcctta ataacccctt tcaacgagaa aatcccatgt
      121 cagaaattac atccctggtc actgcagagg cagtgaagga agtcctgcgc tctgaagaag
      181 tcctgagcgc actgaaacag aaactccgcc agaaccttga ggcgcgtctt gatgcagaag
      241 tggatgccat tctggatgaa ctgctgggcg caccggcagt tccggagccg gaaggtatcg
      301 cgggtgaggg gagtgcttca gatggcggtg accccacacc tgacagcgac atgatgatgt
      361 aagcatgcgc aagggaccat cggtgtgtgc cggtggtctt tatattgttg tgagcttccg
      421 gattgcggga gacggggtat gtaccagatg gaaaaaatca caacaggtgt gtcatacacc
      481 acgtcagcgg tgggaacggg ctactggttc ctgcagttgc tggacagggt ttccccgtct
      541 cagtgggcgg caataggtgt gctggggagt ctgctgtttg gtctgctgac atatctgacg
      601 aacctgtatt tcaaaatcag agaggaccgt cgtaaggcgg cgcggggaga gtagacgatg
      661 aataaaaaat atgaactggt tgtaaaaggg ataaatcatt actcggataa gataactgtt
      721 actgtgacac tggaaattgc cgggcagccg tcgttgttgt cgccatatgt ggcgattagt
      781 cttgaccgta ctgaaggtgc cacgctggaa ttttacgaag ctgaggcgaa aaaacaggcg
      841 aagcagtttt tcatggatat tgctgccgct ttatgtgaag gggatgaaca gtcacaggaa
      901 aagcgcctct gttcagaaga gcggtatacc atacaaatta acaatgcata taacacgata
      961 cagtcagaaa aagatgatat cgaatcacgc attgaaaaac tggagaacag cgtcgttgaa
     1021 ctgaataaaa aactgtcggt gcagatccct tccgaaggtg aaaaaaaacg ccgtgatgag
     1081 cagttcgcgg cgttttacga ttattgccgg aaagtgatga gcagaaatct cgcagagtgt
     1141 ttccgtattc aaaaaaacag agagcgctct gcaaatagtg agattctgga taatttttta
     1201 aaggggacgc atctcgccag tgattgtttc attgctcatt cttttggtgg ctttcatgag
     1261 gccatcaaac atgatgttga gagaagtcca ggtatcctct gttccattcc cgtaagcatc
     1321 gaaatagaca caaatacgat aaccggtgaa ctcgttaccg ccggagtcgg tatattccgt
     1381 catgagggga acgatgccaa aggttccttt acaaaaatcg ggccgttctt tctgaaggta
     1441 gagcgatagt ttgttaagta cccatccgac agctctctca ttttggttta ttttcgtatc
     1501 gcgaaacaaa aattcgaagc ggccagatga cgcatgggtg caaccaaata ccggggcgca
     1561 ttcatcaacc tttggtgttc ctgaaaacat gcagtccgtg ctctcgaatg cagatgaatt
     1621 gctgttgatc cacttaacaa aagaaaaaag gtctgaatcg cttgtctgtc tgaatattca
     1681 acaaaatcct caaggtgtgt ctcgatcagt tccgggtgtt tacgaaagtc ataatattga
     1741 ccgggacgat atttatgagt ggttgtttta tcccatggtt gtgctctgcg tccctgtaaa
     1801 cggaggtctg ccgctggata gataagcata agtaatctcc tggtggaatg taatccacga
     1861 tcatccgggg aaattaagaa cccgccagtg cccaccactg gtggggtgaa ggcttaacat
     1921 atccgggtat tcggaaccga taaatcctga taaatatcca tgaacgcaaa aatcagatac
     1981 ggcctgtcgg ctgccgttct ggcgctgatt gccgcagggg cgcctgcgcc tgatattctc
     2041 gaccagtttc tggatgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggt
     2101 atctggacca tttgtcgtgg tgccaccagg gtggatggca aacctgtcgt cccgggcatg
     2161 aagttgtcga agaaaaaatg cgaccaggtt aacgccattg agcgtgataa ggcgctggca
     2221 tgggtggaga aaaacatccg ggtgccactg accgaacccc agaaagcggg gatcgcgtca
     2281 ttctgcccgt ataacattgg tcccggtaag tgtttcccgt cgacgtttta taaacgaatt
     2341 aatgcaggcg atcgcagggg agcgtgtgag gcgattcgct ggtggattaa ggacggtggc
     2401 agagactgcc gtattcgctc aaataattgc tacggtcaga tatcccgtcg tgaccaggag
     2461 agtgcgctgg cgtgctgggg aatcgacaga taagcagaat attttgctga aaaatgacgt
     2521 tggtcaacgc gggtggataa cacgaaatcc tgcgaactgg caaaacctaa gtgaataaag
     2581 tcaggaacat tgtttcacgc agaggcaccg taatggtgcc tttgtcattt ctgcgcttcg
     2641 cacaagcgta aataaaccaa agaacctttc aggatgagcc ctggtggata accggcagtg
     2701 gtctggttaa ccctctttgg gctggttatt cctgtgcgca gggttcatca ctaaaaggaa
     2761 tcaaccatga aagagatgat ttctgtcgat cgtgaaatat ccatgagtag tctggatttt
     2821 ctgaataaca ttattaatcc agcccgggca gaagccggag aagtccctca tgaaccgcgt
     2881 aagtttcttg caaaaattga ggatgagcta gagcttgatg gaaccggaaa aaaattccgg
     2941 ttaaacaata accagacaag aacggcatac tatgatctgg attttgacca gatgatgctc
     3001 gttggcatga gagagtcaaa ggccgttcgt cgttctgtgc tggcaaggct caaagcgatg
     3061 catggtattc agatccccca gactttacct gaggcgttgc gatttgcggc aaaactggca
     3121 gaacagaaag cagagctgga aaatcaactg gcaatagcag caccgaaggc tgaatttgtt
     3181 gataactatg ttgaagcatc tggtctgatg ggattccggg aagttgctaa gttactcggt
     3241 atcaaagaaa ccgatttccg gctgtttttg ttggagaacg gaataatgta tcgcctcgct
     3301 ggaaaaatga cgccttactc gcatcacctg gatgcggggc ggtttagcgt gaaaacgggc
     3361 gaggcgggca atggtcatgc ttttacgcag gttaaattca ccccaaaagg tgttcagtgg
     3421 attgctggtc tgcttgctgc atggagagct accgcagcat gaagatgata aaaatggact
     3481 ggaaattttt gctggtctgg ctgattccgt ttttatgggt ggttgcccgg ttaattactg
     3541 ctattaagag gtaaagatgt cagacaaact cataatgctg gcgaagggcc tttgtgtaat
     3601 cgtcggtatt tcattttcac taatgctggt tgctcttttt ctttccataa cctggatggc
     3661 gttgacttcg gcagggctgg tggggtgagc ataaaccgaa tgctttccgc gtttaccgtt
     3721 attctgctgg tggtctgtgg tgcgcttagt ctggggctga atcattaccg cgata
//
LOCUS       DAZGBC010000285         3751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGBC010000285 DAZGBC010000000
VERSION     DAZGBC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.287"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            381..623
                     /locus_tag="ACHK1J_004954"
     CDS             381..623
                     /locus_tag="ACHK1J_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914091.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(716..2251)
                     /locus_tag="ACHK1J_004955"
     CDS             complement(716..2251)
                     /locus_tag="ACHK1J_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7914092.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSACYAPVGMIYGKINNSFIASVMQ
                     TNLNEAGEFITGIPVLYFIVSLIIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKYFLKEGSSSSRRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHPNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(2451..2906)
                     /locus_tag="ACHK1J_004956"
     CDS             complement(2451..2906)
                     /locus_tag="ACHK1J_004956"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFO7914093.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVIYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLAALCLFFIYSFFKRKRVVGKLLLCIYILYISHYA
                     ITFVYCLYVVGAECMTTGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(2994..3617)
                     /locus_tag="ACHK1J_004957"
     CDS             complement(2994..3617)
                     /locus_tag="ACHK1J_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7914094.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
BASE COUNT         1173 a          891 c          701 g          986 t
ORIGIN      
        1 cgtcacacag acgacaatta acattgttaa aaacgttcgt tgtggcatcg cagcccctcc
       61 attgcctttc ggccgtcatc gggctactat aatgttgtct aggcattaca gtgggccctg
      121 ggttgattga taaaaaaatc gcctggggct ttctgctgca tgccgtccac aaaacgcttt
      181 cagcatacag cctcaagcgc ccgccgctat tctaatcgat tttcagtctg ccacaatcgt
      241 tttccaccta atgacatgtt atgcacaaca gtatttttac gggaaactac acggcaagga
      301 ttcccggaac acgttcatta tttgatggtt acagacatca tgttaaatcc ggaagtaata
      361 atgacctgaa aggaaatact atgagtaacg aatatcaaag caccagtata aaccctgatg
      421 tttttgaaca atttcttacc ctcctgaata cacactgtaa atcatcagaa gctgcacttg
      481 ccatgctgag tacactctgc tacgaactga tgaggaggag tgggatccat tcactggaca
      541 gcctgatgct caacggagaa ggtatattga tacaattaca gggggctgat attcgcgaaa
      601 aaacaggtca gcatattcac tgatccaggt atgggctgaa aacagtgtca gcctgaaata
      661 aacgctcaat gcctcataca catgaggcat tgagctcggt cataaataat ctttattatt
      721 tttctgattc agcatttccg gtcagtatcg gctcttctgg caaagacatt accggaacgt
      781 cacgttcacc attgaataca gaaacacttt cagcgaaatc attctgtaac aaagaaaaac
      841 ttaacggcag atttttggtt tgtatccctg tgatccattg aaaaatctcg ggaaaacgac
      901 gtgcactgat atgcacatga ttgaggtggt gctcagtaat atcactggcc atcatcatga
      961 gaggtacatc gtatcccgaa cgcaaacctg gcgcatgatg cacaggggtg ctgtctgaac
     1021 tgacggattg cccatgatca gaaaaataca gcatggcaaa atcatcgcca tgctctgtcg
     1081 tcatgttata aattgcctga agaaaccgat ccagcttaag caatgttgat aaataacagg
     1141 atactttatt tccgaataat tccctgaacg gctgtgcatc attaaaaagt ctgtcacagg
     1201 gattaggatg ggaccccagc atatgaatga aaatcacttt tggagaagac gaattaacag
     1261 cttccctgac atactgaagc atatcttcat catcaatgcg acgggaagaa gagctccctt
     1321 ccttaagaaa atatttctta tccgcattgg cggcgatgac agaaatcagc gtatcatgtt
     1381 gccctaaaga gccctgattt gatatccacc acgtctcata cccggctttt ctggcaagcg
     1441 taacaatatt attacccgga ttacctgttt cagtgtaatc aagcgtcaga gttctgctga
     1501 gtgaacctat cgtattcggc gctgcggaga aatacccatt aataaatata cccggagcag
     1561 aattcagcca tggtgttgtc ggcaaaggat acccataaac agaaagataa tcccgtctga
     1621 cactttcacc aattacgata attatggttt tatatttctt ctccacactc acaatatccc
     1681 atgagtcttt ctgattgttt ttcagcgatt catatcgcat catttcttct ctggtttcag
     1741 ctatccctgt cattacattc gtaaccatac gttttggcca tgcatttact gacaatacga
     1801 taaagaacaa cagaaagata aatccccggt ggcttttatc acgcttacgc cagaaccaga
     1861 atgtaaaagc agcaatgatc agtgaaacaa taaaataaag aacaggtatg cctgtgataa
     1921 attcacctgc ctcatttaga tttgtctgca ttacagacgc aataaagctg ttgtttattt
     1981 ttccgtaaat catcccaacc ggagcatagc atgcacttaa caaaagaaag atacagacac
     2041 acgttgctga aataacattc tgccgcccca ataacaacat caggaacaat ataagaacat
     2101 tccccagggt gtttttcact gtatatccgc aacaccatgt aacaagcaca gaaaacaaca
     2161 gataaatcag tgactcttta caccacaaca taaaataaaa ctgttttgct ctctcaaaag
     2221 catattttga tttttcttta atacaagaca cgccagtcat tttcctgtac aaacacagac
     2281 gatcatttca acgcatggat atttatatga aacagttcac aaaaataccc taccctgtcc
     2341 agcaagattt acatattatc aggttctgtg tttacagcaa ctgctccccg aaccagatta
     2401 ttcgcataac ccgatgaagg cctcataacc ataaaacact cagaatacat ttagttaaca
     2461 aaaatatgtc ttgccctccc ggaaaggcaa acatacgaaa gagaaagcat ggctataata
     2521 atatcaatca aaatatgctc aatctttccg gttgtcatac actcagcacc gacaacataa
     2581 agacaataaa caaaagtgat agcatagtgt gatatataaa gaatataaat gcacagaagt
     2641 aatttcccga caactctttt ccgtttaaag aaagaataaa tgaagaacaa acataaagcc
     2701 gccagaaaaa gaaatgtagc tatagatatt ataaaatact ccgtcacctg tgaattctca
     2761 tccagagatg aatacagcga ataatatgtg gttgtgagat taagccttgt tatcaatata
     2821 taaataactg ataatagtat aatataaaga atcagaccaa atctgttttt ctgcgctccg
     2881 gttaatactt ctgatttatc tcgcataaaa tatcctgtct gtaatctgtc ccggtaaaca
     2941 atggtaaatt tcagaatcag tgcgaatacg gtaacaaaga ttcctcctgt gctttaaagt
     3001 gtgtaggcag aaaccagtct ggtggttccg gtcagggagt ctgatacctc aacttcccgg
     3061 gtgactataa ccatacctga ctgacgcagt ctctgcacat cagctgcaat tcgctgagtt
     3121 cccagccaga acaggccatc cagtgccgtt accagcaacc cgctttccag cgccagtttc
     3181 agccgtgccg tcggggcttt aacctgcccg gcgatatcag taaaacaacg cgtcgtcatg
     3241 ccatagcagt ccatccgccg tattcgcgtc gcaagactgt agctgaaaca atccggcacg
     3301 ccggataagt cattctgcag actgtataca catccccgct gccgcccgtt gaacagggct
     3361 tttttctgac tgagcagcca ttcccgacgc agagtgtcaa tcatctgcgg tgtccgggtt
     3421 aacagcagac gaaacggcag ctcaaaactg gtcacataat caacattcag taatgctgtc
     3481 cgcagtcgct ccctggcacc tgccccggtc tcccggcatt cacgcatgac atcagcccac
     3541 tgacgcatca gtcgtggaga ctcattcctg gccctgaact gatatttctc aatatgataa
     3601 tcaatacgtt cctgcatatc gccaccggat atctgttgtt tacccagaca cagtgattag
     3661 cctacaggaa taaatatcct ccgtgaagcc aggtgcctca gaagataaaa acatgtactt
     3721 ttttcaacgt gtaatattaa caccaccacc a
//
LOCUS       DAZGBC010000286         3739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGBC010000286 DAZGBC010000000
VERSION     DAZGBC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.288"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1301)
                     /locus_tag="ACHK1J_004958"
     CDS             complement(<1..1301)
                     /locus_tag="ACHK1J_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFO7914095.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPP"
     gene            complement(1399..2409)
                     /gene="iolG1"
                     /locus_tag="ACHK1J_004959"
     CDS             complement(1399..2409)
                     /gene="iolG1"
                     /locus_tag="ACHK1J_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFO7914096.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(2427..3332)
                     /gene="iolE"
                     /locus_tag="ACHK1J_004960"
     CDS             complement(2427..3332)
                     /gene="iolE"
                     /locus_tag="ACHK1J_004960"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFO7914097.1"
                     /translation="MYSVKESIKWGIAPIGWRNDDLPEIGKDNTYKQILSDAALTGFS
                     GTEVGGCYPLDPTELNIELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVH
                     AYIAVVSEQTYSIQGIIDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHH
                     HMGTGVQTLQEVDRLMENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKD
                     VRDEKLKACRAGKKSFLNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQD
                     PNIYNPLEYAQKGKKHIDGLLGNYI"
BASE COUNT         1119 a          884 c          669 g         1067 t
ORIGIN      
        1 ggcggcagcg ttaatgcgag ttctctgtcg ccatcgacca cccggaaatg cctttctcgc
       61 gtatcgcgat tatataccac taaaactcgc tcaccatcag gattaacaaa acccacatct
      121 tccagttgcg tatcataact ggaagtaagc ataactttcg caccgggatg cacaaagcgg
      181 caaaagtgtc caattccgta ccaagagtgg ttacaccaga gaatgtcatt caatgcatcg
      241 tactgcacag gagcttcgca caaattactc tggtgatttg ggcctccatc actgtctaac
      301 agcaggttcc agtcgataaa tccgctacag ccggatttaa aattaccaat catgtcatga
      361 atataggtat gccagtgacg gatctggcca ccttcatcat tctccattgg tacacatcct
      421 tcggaaaaca gcagcttttt attcggcaac ttccgggcca gatactgaat ttgcgaaaaa
      481 tggtcaccgg tataccagtg gaaagccatc ccgttgatgc tatcgccatc cttttcgtca
      541 gcaaaggcac gttcagccca gtcaatcaga ccatctttat catgatccca gatatagatc
      601 tctatttcat tcattccata gcgggcgagt cgtgggcgca gatagtgaac agcaaaagta
      661 gtctcttctt ctacactata cagacaggaa tcccagcttt ttatcgcaac tggttcattc
      721 tgcactgata gtgctttaac actgatacca tggcgtctgt attcgagcag atagtcgata
      781 ataatatctg cccagtcagc atagtattca taccgcaatt tcccacctcc gttcatatca
      841 taattagtct tcatgaatgc cggtggactc cacggggaag ccattaattt tatttgtgga
      901 ttgatccgca atgccgcgga aattagcggc attaaatgag attcatcacg ggaaaaggaa
      961 atccttccct gacgcaggtc ttcactggaa tcaacatagg cataattgct cagagagaaa
     1021 tcacagcttt ggatcggcat ccgccccaaa ctatagttat gttcctgagg cgaaaaatag
     1081 agattcaaaa attgcgcctt tgtcttatca ctcatgctgt tgaaaacaac accagcactt
     1141 tcagtgaagc tgccgccaaa tccatcaatc tgttgtaacg agtggtttgg aaagacgctg
     1201 attaattcac tacacgctgg ttgacgaggc gaaaaaaaaa cctccagctc aaccataaat
     1261 tgctgattcc agggatcgga agtgattagt ctgcctttca tagagggatt ccagataata
     1321 taggatgata aattgtgata taccggacag gagcatacac ctagctatcc ggtatgaaat
     1381 tatcacatcg cagaaagact atttgtagaa gtcaggtttt gctggtaatt ccacaaactc
     1441 ggtgtttcct gtttcctggg acttcacaca ggcatcagcc gttaccgcag ccagataacc
     1501 atcccatgat gttgggcctg ccagaggtaa tcccgcattc agacggtcaa agaagtcctg
     1561 gaactcaata tcataggcat caataaagcg ttgtttccag tccaccaaaa tgtcagtact
     1621 gtattttgcc tctttacgca ccgcagcagt agccacggta ggtaattcca ccatcccttt
     1681 ctcacaggtc acatcacaat gaatatcata gccatactgg caattcacaa acacctcaac
     1741 cactatatta ataccggagg tcgtttccat caccaccacc tgcggatctc gtagagtagt
     1801 caccaagctg gactgacgtg ggaagtagac cttaactgtt ctataatctt cgttgagtaa
     1861 ccagtgcata acatcgattt catgaatcag cgtttcataa attgcttgtg gtgttttgta
     1921 atccgatacc gtgcttacat tataatgacg accatgaacc attaatggtt gaccaatttc
     1981 tccgctatcg atgatgtttt tcagctgtac gtagccttta tcgtagcgac gcataaaccc
     2041 aacttgcacc attcgttttc catttttacg ttcagctccg atcacacgct gacaatcatc
     2101 tgctgtaacc gcaagcggct tttcacagaa aacatattta ttggcattta acgctgcaac
     2161 ggctacatcg gcatgtactt cattcgaggc agtaattacc actacttcga catcttcatc
     2221 attaatcaga tcgtgatagt ctttatagtc ttttgctttg atctcatact tatccaaagc
     2281 tgcttgcgct ctgccttcga caatatcgca cacagcaaca acttctactc cagaaatagt
     2341 gttagccaga cgtcttaagt gatcggcacc aattttcccg atccccacaa tacctgcttt
     2401 taaagtcatc ttattttcct tttctatcag atataattac ccaataaacc atcaatatgc
     2461 tttttacctt tttgtgcata ttccaaagga ttatatatat tcgggtcctg ctcagcttca
     2521 acgacaatcc agccggaata atgattctca accaaataag ccaatacaga tttaaaatca
     2581 atattcccat caccaggaac agtaaacatt ccatcaagaa aagaattgag aaatgatttt
     2641 tttccagccc gacatgcttt tagtttttca tcccgaacat ctttaaagtg aacatgttta
     2701 atacgggaaa aatgcttgca caacagtgac atcacatctc catcagaaac ataaatatga
     2761 ccagtatcaa acagcaaatt cactaattgt ggatctgtgt tttccataag tcgatccact
     2821 tcctgtaaag tttgtacgcc agtgcccata tgatggtgga aagcaagttt aagcccatat
     2881 gtattggcga tcatgcctaa ctcgttcaat cctgagcata attgttgcca ttcgatatca
     2941 ttgaagtaag gtttttccgt atagacacat ttatcaataa ttccttgaat actatacgtt
     3001 tgctcagaca caacagcgat ataagcatgg acagcctgca aataagcgca atgttgttca
     3061 aaagctttct tcactgattc aataccatca cgaataataa aagatgagaa ccactttccg
     3121 ggaatttcca gatggcgaag tgctaactct atatttaatt cagtcgggtc cagaggataa
     3181 catcccccca cttctgttcc agaaaaacca gtaagtgctg catcacttaa tatctgctta
     3241 taggtattgt ctttaccaat ttcaggcaaa tcatcatttc gccatccgat tggcgcaatc
     3301 ccccatttaa tactctcttt tacactatac atattccaga ctcctgctta ataaaacttt
     3361 ctcacatcct gttgcataag taataatctt tggctgacaa tatgctactt tccacgtggc
     3421 taaaaaactt tatctatcat gagtacagaa gtgggaaaaa tgtgtcagga atcacaaaaa
     3481 aaacacaacc ctttaatgat tttgtctatg ttttagtcat gctgaaaaat cacacaatga
     3541 ccgtaaaata aaaccgtcac tttcccacta actctggcgt ggacagaatc atcccgtatg
     3601 attcccaagt ttggtaaata ttattaaatc aggatgtgca agctatgatt cagtcatact
     3661 aggaaaaaag tttttttgca gattcgattg gtatcaatga agacgatata taataatatg
     3721 caggatagga aaagttcag
//
LOCUS       DAZGBC010000287         3631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGBC010000287 DAZGBC010000000
VERSION     DAZGBC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.289"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..1344)
                     /locus_tag="ACHK1J_004961"
     CDS             complement(34..1344)
                     /locus_tag="ACHK1J_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFO7914098.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
     gene            complement(1357..2628)
                     /gene="dcuC"
                     /locus_tag="ACHK1J_004962"
     CDS             complement(1357..2628)
                     /gene="dcuC"
                     /locus_tag="ACHK1J_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7914099.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
     gene            2764..>3631
                     /locus_tag="ACHK1J_004963"
     CDS             2764..>3631
                     /locus_tag="ACHK1J_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7914100.1"
                     /translation="MKYMPKLQQIKFFNEIVLCGSLRAAARKMNISQPALSRTIKELE
                     NVLGCNLLIRTRDGIVLTEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGIS
                     SLFGVTILNNVLDNFRMKYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGD
                     FIVVELFNAPFTIVARKHHPLRNCTSIDELAAARWVFPETQMGYYHYISRLIPFGHPD
                     EPYAPIFTDSVVCIMNLVLYDDYITVLSRARLQEARFKEILCSIPVKYQLPVSQYGLI
                     YPRKRLMTAPAQI"
BASE COUNT         1148 a          772 c          691 g         1020 t
ORIGIN      
        1 atcaggagtg attgacagga tacctgtcag caatcaatac aattcagatt ccagttttcc
       61 gttccctaca attttattta gtccgggaga gttcaatctc tccagacata acgaaaacag
      121 tttaacgcct gtatccagca cattttcatc aaaatcaaaa tcaacttcgt gatgaccagc
      181 ttttaaatca ctgccaagta ttatatatgc tgcttttcct cctatcttct gtacacggcg
      241 aataaggagc gttgcatctt cactgccccc aaatgatttc tctgtcaaag acatgttcat
      301 atcttcttgt gctatatagt gtatcagatt gacaagtaaa gggtcattat gtaaatccgt
      361 tgcttcacca accactttgt actcaaaacg taaaccaaat atggaagaaa taccttgtgc
      421 tattttcaga gcatgttcac gcatgtattg atttattgtt tccgtttctc cacgaacttc
      481 aagttgcatc cttgcaaaag acgggattat gttccttcct tcacctgcgt ttagcgtgcc
      541 aatatttatc cgggacatac cttcaccatg gcgtggaatg gcaagtaatt gcgttgcaca
      601 gtgacaagca gctgccagag cattatttcc caggtgagga tttacaccag catgtgccgg
      661 agtaccatag aaaaagaaat ccatttttgt cgtacacaac aaatctgtag gtgaagcgac
      721 aacatttcct gtaggaactc ccataccaat atgtgaagaa aggaagtagt cgcagtcatc
      781 aagaatacca ccttctgcaa taggttttgc accgcgaacg ccttcttctg cagcctgaaa
      841 aattagctta attttcccgc ataacttatg gttattttca gatacccatt gtgccaatcc
      901 taaaccaatc gatatatgac catcatgtcc acatgcatgc attaagccag agtgtatgga
      961 tgcaaatccc attttttcag gaatatgatc tttcgatttt gattcactta cctgtacaca
     1021 atcaatgtca aaacgaaacg cagtgacagc tccatttttt cctgtatccc ataaagcaac
     1081 acaccctgta aagccatcca tttcatccag caattcatgg ggaacgcctg aatcaactgc
     1141 acgttttata ctatctgcaa cttcagttgc attacggccc ataatatact ctgttgacag
     1201 gtatttattc cccgtaataa cctgccatcc cataccacgc agaatagtaa ttatctttga
     1261 cgttgtgata aattcgcacc acccactttc cggaatatgg tgaaattctc tccgccattt
     1321 ttgtaactga ttcagttcag acatatacgc ctcctgttaa aggaataata atgcaataaa
     1381 caataccgtc acaatcattc cgggggcagt gcgttttgca atttcaactg gattaactcc
     1441 agccagaccg gcacagataa tagttgcacc agccaacgga gacatgtttc tccctagcgc
     1501 agaagccagc ataactgcag cacccagagt atttgtatcg taaccaaaat tgttcgcatg
     1561 tggtgtgaca acttcgttga atgcgaatgc agtcgcatcc cctgtgccgg ttataagtcc
     1621 catcaggaat ggccccaatg taccgcccca gcgggcaaat tctggtgagt cagtaagaaa
     1681 tctgataaac acatcaatga taccaacaga tcttaaacct tcaatgaaaa cagctgcgga
     1741 aattatgatc ccaagaatat ttgcatatga tgtccccata ccctcaaaga agctggaaac
     1801 taattctttg ggattggatt tactgacaat cagggcataa agacaaccga gaatcattgc
     1861 ggctggaacg gttattttag tctctccaag aaaaccaaat tcagccagca taagtaatgc
     1921 tacgggaaga aatggtgcta cagatttcag ataaaaacag atatcacggt cagtgaactc
     1981 aacctgtgtt tgttctgata tgtttcctgc agaatcacat tttgtttctt ttcttacaat
     2041 agcaacaact gtaagaataa cagcacatat tacaccacat gttattgtag tcggagtatg
     2101 gaatacgacg aattccatta atggtgtttg tgatatttta gatataaatg aattatgtgc
     2161 aacaccggga ttgaggtaag caccgatagt tcctgaaagg atagctgctc cggctattgc
     2221 aggatgaata cgtgctgaaa taagtaatgg tatcagcgtt gcaccaacag cagctgatgc
     2281 tccagcagca gatggaatcg cgatgcaaac aataaaactg acggtaaccg cagcaggaat
     2341 aagaaagata ccaagacgag ataatccttt cgttagaaaa tgaaccaggc atgtatcaca
     2401 accagtgaat ttcataacac aggcaaagcc catactggta caaatggcct gaatgagacc
     2461 ggactcagtc attctgcttg caaatgccga aaatgcgttt aaaggtgaca tggcaactat
     2521 acatagcaga atacctgcac caattagtgt cgttcgtgtg tcgtatcttt ttatcaaaag
     2581 cacaactgtg attatcagaa tcacacatcc agttatcacg ttaaacatga gatcctcctt
     2641 cttcaaagaa ggataatctt tactttatgt gatttgctca aatattgatc atgaacaaaa
     2701 aaatcaatta accggggata ctattatgat ggaggtgata acagaaaatt atcaggcgaa
     2761 aaaatgaaat atatgcctaa acttcaacaa attaaatttt tcaatgaaat agtcctgtgc
     2821 ggaagtttaa gagctgctgc ccgtaaaatg aatatatctc agccagcatt aagcagaacg
     2881 ataaaagaac tggagaatgt attaggatgt aaccttctga ttcgaacaag ggatggtatc
     2941 gttttaacag aagctggaaa gatattctcg gtccattcac gtatgattct tgaagagatt
     3001 gaaactgcag ctcttgaagt tgcgaaagta aatatagata acacaggaaa agtagctttt
     3061 ggaatttctt cgctgtttgg cgtaacaata ctgaataatg tacttgataa cttccggatg
     3121 aaatatccga aaaagcgtgt gatgataaga gagggacagt tgtcctccct tatccctttc
     3181 ctgagagaag gtgttctgga ttttgcaatg ggtacattaa caaaagacat gccagttgga
     3241 gattttattg tggttgaatt gtttaacgca ccgttcacta tagtggcaag aaaacaccat
     3301 cctttaagga actgtacatc aatcgatgaa cttgcagctg caagatgggt attcccggaa
     3361 actcaaatgg gatactatca ttacatatcc cgtcttattc cctttggaca tccggatgaa
     3421 ccatatgctc caatatttac cgattcggta gtttgtatta tgaatcttgt tctctatgat
     3481 gactatataa cggttctctc cagagcaagg ttacaggaag caagattcaa agaaatattg
     3541 tgttcaattc ctgttaagta tcaactaccc gttagccagt acgggcttat ttatccacga
     3601 aaaaggctaa tgacggcacc agcacagata a
//
LOCUS       DAZGBC010000288         3627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGBC010000288 DAZGBC010000000
VERSION     DAZGBC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.290"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            235..1128
                     /gene="nanA"
                     /locus_tag="ACHK1J_004964"
     CDS             235..1128
                     /gene="nanA"
                     /locus_tag="ACHK1J_004964"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015962808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO7914101.1"
                     /translation="MTADLKGIMSALLTPVDAEQKLDQDSLRRLTRFNIEQGIDGLYV
                     GGSTGEAFVQSVQERESVLEIVADEAKGKLKLIAHVGCVSTNESQSLARAASRLGYDA
                     VSAVTPFYYPFSFEEHCEHYRAIIDAADGLPMVVYNIPALSGVKLSLEQISTLVTIPG
                     VRALKQTSGDLYQMEQIRRSHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYK
                     RIAGALQNGDIKTAKYLQSECNKVIDLLIRFGVFRGLKTVLHYMGIIDAPLCRKPFSG
                     VNAEYMPELKSLAETLMKEKT"
     gene            1209..2696
                     /locus_tag="ACHK1J_004965"
     CDS             1209..2696
                     /locus_tag="ACHK1J_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7914102.1"
                     /translation="MNTLQNKTPWYKHLNRSQWRAFSAAWLGYLLDGFDFVLITLVLT
                     EIQGDFGLTTVEAASLISAAFISRWFGGLVLGAMGDRYGRRLAMVVSIILFSLGTLAC
                     GLAQGYITLFIARLVIGMGMAGEYGSSVTYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRVLFFIGILPIIFALWLRKNIPEAEDWKEKHEGKAPVRTMVDILY
                     RGEYRIANIAMTLVAAVALWFCFAGNLQNALIIAGLGVLCAVIFISFMVQSSGKRWPT
                     GVMLMIVVLFAFLYSWPIQALLPTYLKTELSYDPSTVAQVLFFSGFGAAAGCCVGGFL
                     GDWLGTRKAYVFSLLASQILIIPVFTIGASSVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGAVAPVLGAVVAQRLDLGTALCSLSFGLTFVVILLI
                     GLDMPSRMQRWLRPEALRTHDAIDGKPFSGAGVIKNESSLEMKSR"
     gene            2720..3105
                     /locus_tag="ACHK1J_004966"
                     /pseudo
     CDS             2720..3105
                     /locus_tag="ACHK1J_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3105..3209
                     /locus_tag="ACHK1J_004967"
                     /pseudo
     CDS             3105..3209
                     /locus_tag="ACHK1J_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(3441..>3627)
                     /locus_tag="ACHK1J_004968"
     CDS             complement(3441..>3627)
                     /locus_tag="ACHK1J_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO7914103.1"
                     /translation="MFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWP
                     SNRVDELLPWNVVLTNK"
BASE COUNT          941 a          695 c          891 g         1100 t
ORIGIN      
        1 tataaaataa caaatcatta gatatgctaa tgtttagaat tggcctgagg gtcgatttaa
       61 attgttttat ccagatgaga tttcaatatt tgcattgata tatttgtacg tttctggcaa
      121 ctggcagaca aattttgtaa ccattatcac aaaaccattt tttccatatg gatttgtaca
      181 gaaccactgg tgtaataggt ataaaggtat actgaattgt tgaagaggta aacaatgact
      241 gccgatttaa aaggcattat gtctgctctg ctgacacctg ttgatgcaga acaaaagctg
      301 gatcaagaca gtctgcgcag gttgacaaga tttaatatag agcaggggat tgatggattg
      361 tatgttggtg gctctacggg agaagccttt gtacagagtg tacaggaacg tgagtcagtg
      421 ctggagatcg ttgctgatga agcaaaagga aaactgaagt tgattgctca cgttggttgt
      481 gtgagtacaa acgaaagtca gagtctcgcc cgggcagcaa gcagactggg ctatgatgct
      541 gtttcagcag tgacaccttt ttattatccc ttttcttttg aagagcattg tgaacattat
      601 cgtgccatta ttgatgccgc agatggatta ccgatggtgg tatataacat ccctgcactg
      661 agtggagtaa agttatctct cgagcaaatt agcacactgg tgactatacc cggtgttaga
      721 gcacttaaac aaacctccgg agatttatat cagatggagc agattcgtcg gtctcatcca
      781 gacttggttc tttacaatgg ttatgatgag atatttgcat ccggattgct ggcaggtgca
      841 gatggcggga taggtagtac atataacatt atgggatgga gatataaaag aatagctggt
      901 gcactgcaga atggtgatat aaaaacggca aaatatcttc agagtgaatg taataaagtt
      961 attgatcttt taatcaggtt tggtgtgttc cggggattaa aaacagtact tcactatatg
     1021 ggaatcattg atgccccatt atgcagaaaa ccattctctg gtgttaatgc agagtatatg
     1081 cctgaactta aatctctggc agaaacttta atgaaagaaa aaacataaag tacggttaca
     1141 tattctttgt tatataaagt ttaagataac tgaataaaca ggaacatctg tatgtgaggt
     1201 tgttatttat gaatacccta caaaataaaa cgccatggta taagcattta aatcgtagcc
     1261 agtggcgtgc attttctgcc gcctggctag gttatttact cgatggtttc gattttgtgt
     1321 tgataactct tgttcttacc gagattcagg gagactttgg cctgacaacg gtcgaggcag
     1381 ccagtcttat atccgctgca tttatttccc gttggtttgg tggacttgtt ctgggagcaa
     1441 tgggagacag atatggacgc cgtctggcga tggttgtcag tattattctt ttttcccttg
     1501 gaacactggc gtgtggcctt gctcagggat atataaccct ctttattgct cgtctggtta
     1561 ttggtatggg gatggcaggt gaatatggca gtagtgtaac atacgtcatc gaaagctggc
     1621 caaagcatct ccgcaataaa gcaagtggct ttctgatttc tggcttttca gtaggtgcag
     1681 ttgttgcagc tcaggtttat agtctggttg taccagtatg gggatggcga gtgctgttct
     1741 ttatagggat tcttcctatt atttttgctc tctggctgcg taaaaatatt ccggaagcgg
     1801 aggactggaa agaaaaacat gaagggaaag cacctgtccg tacaatggtg gatattttgt
     1861 accgtggtga atatcggatt gcaaatattg ccatgactct ggttgctgct gttgctcttt
     1921 ggttctgttt tgccgggaac ctacaaaatg ctctgataat tgctggtctg ggcgttttgt
     1981 gtgcagttat ctttatttca tttatggttc agagtagtgg taaacgctgg ccaacaggcg
     2041 tcatgctcat gatcgttgtc ctgtttgcgt ttctttattc ctggccaatt caggctctgt
     2101 tacccacata tctgaaaaca gagttgtcat atgacccttc cacagtcgct caggttcttt
     2161 ttttcagtgg atttggtgct gcagctggat gttgcgtcgg aggttttctg ggagactggc
     2221 ttggaacccg taaagcctat gtatttagtc tgctggcttc ccagattctg atcattcctg
     2281 tctttaccat tggtgcatcc agtgtatggg tactgggttt attactcttt tttcagcaaa
     2341 tgttggggca aggtattgcc ggtattcttc caaaacttat tggcggttat tttgatactg
     2401 atcagcgtgc tgccggcctt ggattcactt ataacgtagg cgctctgggg ggagcagttg
     2461 ctcctgtact gggggccgtt gttgctcaac gccttgatct cggaacagca ctgtgttcgc
     2521 tctcgttcgg tctgaccttt gtagtcatcc tgctgatagg tctggatatg ccttcccgga
     2581 tgcagcgctg gttaagaccc gaagccctgc gtacacatga tgctattgac gggaagccat
     2641 tcagtggtgc cggtgtgatt aaaaatgagt cttctctgga aatgaaatcc cgctgacccc
     2701 tgtaacaaac ggagtagtta tgacattacc acagcttatg gacgaaaaag taaaaaaaca
     2761 gggatgggtg atcgtttcct gccactttgt tcaggctgct ccaatggatg atccagagat
     2821 gatcacggca atggctgact gttcttcaga acaggatggt tcgaaatgtt acagacaggg
     2881 cacagaaatt attgtaacca gcctgtctgg ttacacaggt gatcatactc cctatctgga
     2941 tttagtcagg agattaagca agaaaggttg ccgcgttatt actgataagc ccccggcttt
     3001 ggctgcagca atggaacctg ggctgtaaca gtgggggctg ccattactca tcaggaacac
     3061 atctgtcact ggtattgtaa agccattcag gaggctctca gataatgcgt actctggcaa
     3121 tagatatcgg tggtacgaaa ctggcagctg cactggttga tgataccctt aacatttcat
     3181 cccggcgtga aattcccact ccgggaagtt cctgccactt accgtaacaa tgcaggagta
     3241 attggtgctg cattactggc agaaaaataa taaaaaggac attttttata aagaattatg
     3301 ttgattttat agctccacat aactaatcat gtgttcgaca aaaggtatat atatgaaaag
     3361 atatcaagta gtttctggta tattattgtg cgcctcattt tctgttatat gtaagcgtca
     3421 acggagcacc gtattgacgc ttatttattg gtgagtacta cgttccatgg caagagttca
     3481 tcaactcggt tggaaggcca ttccggcagt acgctcagga tatggcgcag atacgcttcc
     3541 ggatcgatac cgttcagacg gcaggtgccg atcagcccgt acaacagtgc tccacgctcg
     3601 ccgccgtgat cgctgccaaa gaacata
//
LOCUS       DAZGBC010000289         3624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGBC010000289 DAZGBC010000000
VERSION     DAZGBC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.291"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..216)
                     /locus_tag="ACHK1J_004969"
     CDS             complement(<1..216)
                     /locus_tag="ACHK1J_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO7914104.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKA"
     gene            449..781
                     /gene="mobA"
                     /locus_tag="ACHK1J_004970"
     CDS             449..781
                     /gene="mobA"
                     /locus_tag="ACHK1J_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO7914105.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            793..3513
                     /locus_tag="ACHK1J_004971"
     CDS             793..3513
                     /locus_tag="ACHK1J_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO7914106.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEVKGEV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVVPGERFRG
                     HSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPELRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAHIRVQFRDPQVRKLHYHIAEVQRMQALIRLK
                     ENVREERLALMVEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNADRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     NDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ISRPDVDLHRQRSEEYYHEYIRQQTRLSESHDENYSSRQENTWVPPSPGM"
BASE COUNT          962 a          786 c         1008 g          868 t
ORIGIN      
        1 tgccttctct cctacttcat accctgcatt tttgagcgtg tcacgaacat ctgccagtgt
       61 ataacccttt gtccttacca ggacaataat gtcatcacga atggcatcaa gaaaatcacg
      121 caatgttttc cgttgagcgg tgaggtccgg caattccgat aaagcagctt ttgccagctg
      181 gatgtcatca tctgagtaaa attttttaga agccattggc actttctctc cgtttcatgt
      241 tgaagcctga ttttatcacc aaaaactgac tcctggcagg gctgcgccct gcctctgccg
      301 tctgcataag actatgatgc acaaaaataa caggctataa tggaccgaaa aacgggacag
      361 gatgtgtaat cgtataaccg cgcacgcacg gctatacgat taccacctgg tcagggctta
      421 gccccgacac cccggtaagg agcagaccat gagtgagaag aaaacccgaa gtggcagtga
      481 gaaacggcaa aagaatgtcc tcattgctgt tcgtttttca ccagaagagg ctgagattgt
      541 taaagaaaag gcagaaaaaa acggactgac agtttcaact ctgataagaa aaactgtgct
      601 gggaaaacag ataaatgccc gcattgatga agattttttg aaagagctga tgcgtcttgg
      661 tcgcctgcag aagcatctgt ttgtggaagg aaaacgcacg ggagataaag agtatgcaga
      721 ggttctcgtt gctattacag aacttgctaa tactctgcga agagatttaa tgggacgctg
      781 agataatcgc tgatgaatgc catcattccg aaaaaaagac gcgacggaaa gtcttctttt
      841 gaagacctga ttgcgtacac ctcagtgcgg gatgatgtgc cggaagatga actcagacca
      901 gacacggaag tgaaaggtga ggttccgcac cggaatcgct tcagacgtct tgttgattat
      961 gcaacacgtc tgcgtaatga aaaatttgtt tccctgattg atgtgatgaa agacggcagt
     1021 cagtgggtga atttctatgg tgtgacctgc tttcataact gtttgtctct tgaatctgcg
     1081 gctgaagaaa tgcagaaagc agcagacctt gctcattttt ccaaagatga tacagatcct
     1141 gtttttcact atattctctc ctggcctgcg cacgaaagtc cgcgttctga gcagcttttt
     1201 gactgtgtgc gtcacaccct gaaatccctt gagctgtcaa aacatcagta tgtggcagcc
     1261 gttcatacag acacggataa cctgcatgta catgttgcag tgaatcgtgt ccaccctgaa
     1321 accggctata tcaattgcct tccgtggagc caggaaaaac tcagccgtgc atgtcgtgaa
     1381 cttgaactta agcacggatt cgcgccggat aatggctgct ttgtacatgc accgggcaat
     1441 cgtattgtgc gcaaaacagc gctggtacgt gagcgccgta atgcctggcg acgcgggaaa
     1501 aagcagacgt tcagggaata tattgctcag atgtcgattg ccgggctgcg tgaagagcca
     1561 gcacaggact ggttatcttt gcataaacgg ctggccagtg atggtctgta tatcacgatg
     1621 caggaagggg aactggtggt gaaggatggc tgggacagag cgcgggaagg tattgcactc
     1681 agttcgttcg gaccgtcatg gacagctgaa aaactggggc ggaaactggg ggaatatcag
     1741 cctgtaccga cagatatttt cagtcaggtg gggacacccg gtcgttatga tccggaagcc
     1801 ataaacgttg atatccggcc ggaaaaagtg gcagaaacgg agagtctgaa acagtacgcc
     1861 tgccgtcatt ttgccgaacg cttaccggca atggcgcgca atggtgaact gaaaagctgc
     1921 ctggctgtac accgtactct ggcagaggcg ggattatgga tggggattca acacgggcat
     1981 ctggtactgc atgatggatt tgataaacaa cagacaccag tacgtgcaga cagtgtgtgg
     2041 ccgttaatga cgctggatta catgcaggat ctggatggtg gatggcagcc tgtaccgaag
     2101 gatattttta ctcaggtcgt tcccggagaa cgattccggg gacacagtat tggctctcag
     2161 cctgtcagtg attacgaatg gtatcgcatg aggatgggca caggtccgca gggggctata
     2221 aagcgtgaac tgttttctga taaggaaagt ctgtggggat acggccttat tcagtgcagg
     2281 agcctgattg aagagatgat agctgacggg gatttcagct ggcaggcatg tcatgagatg
     2341 tttgcgcgta aagggcttat gctgcagaaa cagcatcatg gtctcgtgat tgtggatgcg
     2401 tttaatcatg aactgacacc ggtgaaggcc agcagtatcc acccggattt gacgttatcc
     2461 cgggccgagc ctcaggccgg gccatttgaa attgcggctg cagatatttt cgaacgggtg
     2521 aagcctgaat gtcgttacaa tccggaactt gctgccagtg atgaagtgga gcctggcttc
     2581 cgacgcgatc ctgagctgcg tcgtgagcgc cgtgaagccc gtgcagctgc gcgggaagat
     2641 ttgcgtgcgc gttatcttgc atggaaggaa cactggcgta aacccgattt acgttacggc
     2701 gaacggttgc gggaaattca tgctgcctgc cgtcgtcgca aggcacatat ccgcgtgcag
     2761 ttccgggacc cgcaagtgcg taaactgcat taccatattg cagaagtgca acgtatgcag
     2821 gcgctgatca gactgaagga aaatgtcagg gaagagcgac tggcgctgat ggtggagggt
     2881 aagtggtatc cgttgtccta ccggcagtgg gtggaacaac aggcggcgca aggcgacaga
     2941 gctgcagtgt cacagctccg aggctgggat tatcgcgatc gtcgctcgcg taataaggat
     3001 aaaagacgca caactaatgc tgatcgttgt gtcgttctct gcgaacccgg aggaacgcca
     3061 ctttttaata atgttgcaaa acttgaagct cgtcttcaga aaaatggaag tgttcatttc
     3121 agggatacgc gaacaggaaa aaatgtctgt acggattacg gggatcgggt tgtgttttac
     3181 catcacaatg accgaaatga actggcagag aagcttgatt tgattgcgcc ggttctgttc
     3241 agccgcaacg ggaagcttgg ttttgaaccg gaaggttcat atcagcagtt taatgatgtg
     3301 tttgcagaaa tggtggcctg gcataatgca gcaggaataa ccggaaatgg acacttcacc
     3361 ataagtcgtc cggacgtgga tttacaccgc cagcgcagtg aagagtatta ccacgaatat
     3421 atcaggcagc agacacgttt atcggagagc catgatgaga attacagctc acggcaggag
     3481 aatacgtggg tgccgccgtc acctggaatg taagtccgga tacatgatgg tgcaaaatcc
     3541 agtgaattga attcatcaga gctatatgtc agattagccc gttgaaaaag cttgattttt
     3601 attgagggaa atttttatgc tgcc
//
LOCUS       DAZGBC010000290         3568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGBC010000290 DAZGBC010000000
VERSION     DAZGBC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.292"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..646
                     /gene="ssb"
                     /locus_tag="ACHK1J_004972"
     CDS             113..646
                     /gene="ssb"
                     /locus_tag="ACHK1J_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7914107.1"
                     /translation="MSARGINKVIVVGRLGKDPDVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADATKKSGAKTKGRGRKAAQPEPQPQ
                     PQPPEGEDYGYSDDIPF"
     gene            704..937
                     /locus_tag="ACHK1J_004973"
     CDS             704..937
                     /locus_tag="ACHK1J_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO7914108.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDEQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1001..2959
                     /locus_tag="ACHK1J_004974"
     CDS             1001..2959
                     /locus_tag="ACHK1J_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7914109.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTVEVSVPLASLI
                     KSPLNVRTLPYSVESVSELAESIKGVGLLQNLLVHALPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPQPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQDVLLSLLSFCTACSIHGVQTREYGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            3014..3448
                     /gene="psiB"
                     /locus_tag="ACHK1J_004975"
     CDS             3014..3448
                     /gene="psiB"
                     /locus_tag="ACHK1J_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7914110.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3445..>3568
                     /locus_tag="ACHK1J_004976"
     CDS             3445..>3568
                     /locus_tag="ACHK1J_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7914111.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYA"
BASE COUNT          823 a          948 c         1111 g          686 t
ORIGIN      
        1 aaacccctgt gaagtggacg gcaggaggag ctttcgctcc tctgaagggg cacatgaacg
       61 tcccgtcgcc ggtatccggt ttatctcaca tcttttcagt caggaggttt ttatgagtgc
      121 acgtggtatc aacaaggtca tcgtggttgg tcgtctgggt aaagatccgg atgtccgtta
      181 catccccaac gggggcgcag tggcaaacct gcaggtggcc acgtcagaaa cctggcgcga
      241 caaacagacg ggggaaatga aggagcagac ggaatggcac cgcgtggtgc tgttcggcaa
      301 gctcgcggaa gtggcaggtg aatatctgcg taagggggcg caggtctaca ttgaaggcca
      361 gcttcgcacc cgtagctggg aggataacgg tatcacccgt tacgtcactg aaattcttgt
      421 taagaccacg ggcaccatgc agatgctggg gagtgcacca cagcagaacg ctcaggcaca
      481 accgcagcct cagcagaatg ggcagccaca gagtgctgac gcgacgaaaa aaagtggcgc
      541 gaaaacgaaa ggccgtggac gtaaggccgc gcagcccgag cctcagcctc agccgcaacc
      601 gccggagggg gaggattacg ggtattcaga cgatatcccg ttctgactgg cctgaatgtg
      661 ataaccgccc cgtcctgcgc ggggcatcac cggagagatg aggatgagcg aatatttcag
      721 aatacttcag gggctgccgg acggctcctt tacccgcgaa caggcggaag ccgttgccgc
      781 acagtaccgg aacgtcttta tcgaggatga gcagggaacg cattttcgcc tggttgtccg
      841 caataacagt ggcctggtct ggcgtacctg gaattttgag gacggtgccg ggtactggat
      901 gaaccatgtc atcagggatt tcggcattct taagtaagag aggtgccgga cgggtaggcg
      961 tccggcagac atacaaccac cccggtcaaa ggagaaatat atgtcagtta cagagtctaa
     1021 ggcaaaaaca gagcgcaaat ccagccgtaa acctgcaaaa acgcaggaaa ctgtcctgtc
     1081 tgccctgctg gcgcagacgg tggaagtgag cgtgccgctg gcctcgctga ttaaatcacc
     1141 gctgaatgtg cgcacgttgc cgtattctgt ggagtccgtc agcgaactgg cagagtccat
     1201 taaaggtgtc ggtctgttac agaatctgct tgttcatgcc ctgccaggtg accgttacgg
     1261 tgtcgccgca ggtggtcgcc gactggcagc actcaatatg ctggcagagc gtggcatcat
     1321 tccggctgac tggcctgtcc gcgtgaaaat tattccgcag gaactggcga ctgccgcatc
     1381 gatgaccgag aacggtcatc gtcgggatat gcaccctgcc gaacagattg ccggattccg
     1441 cgcaatggcg caggaaggca aaacacctgc acaaatcggt gatttgctgg gctattcacc
     1501 ccgccacgtt cagcgaatgc tgaaactggc agaccttgca cctgtcatcc ttgatgcgct
     1561 ggcagaagac cgcatcacca cagaacactg tcaggcgctg gcgctggaga acgacaccgc
     1621 gcgtcaggtg caggtgtttg aagccgcctg ccagtcagga tggggcggta aaccggaagt
     1681 gcagaccatt cgtcgtctgg tgaccgaaag tgaagtggcg gtggcgggga acagtaaatt
     1741 ccgcttcgtg ggggctgatg ccttctcgcc agatgaactg cgcactgacc tgttcagcga
     1801 cgacgagggt ggctatgtgg actgcgtggc gctcgatgcc gccctgctgg aaaaactcca
     1861 ggctgtcgct gaacaccttc gggaagccga aggctgggaa tggtgcgccg gacgcatgga
     1921 gcctgtcggt gagtgccgtg aggatgccgg aacataccgc tgtctgccgc aaccggaagc
     1981 ggtgctgacg gaggcagaag aagaacgcct gaacgaactg atgacgcgtt acgacgcgct
     2041 ggaaaaccag tgtgaggaat ccgacctgct ggaagcagaa atgaagctga tgcgttgcat
     2101 ggcgaaggtc agggcgtgga cgccggagat gcgtgccgga agcggtgtgg tggtgtcctg
     2161 gcgttacggt aatgtgtgtg tccagcgtgg tgtgcagttg cgcagtgagg atgacgtggc
     2221 tgccgacgct gaccgcacgg aacaggtgca ggagaaagcg tcagtggagg aaatcagtct
     2281 gccactgctg acgaaaatgt cttccgaacg cacgctggca gtccaggcgg cactcatgca
     2341 gcaaccggac aaatctctgg cactgctggc atggacgctc tgcctgaatg tgtttggcag
     2401 tggagcgtac agtaaaccag cacaaatcag cctggaatgt aaacattatt cgctgaccag
     2461 cgatgcgccg tcagggaagg aaggtgccgc gttcatggcg atgatggcag aaaaagcccg
     2521 tcttgccgcc ctgttaccgg agggatggtc acgggacatg acgacgttcc tgtcactcag
     2581 ccaggacgtg ctgttatccc tgctcagttt ctgcaccgcg tgcagcatcc acggcgttca
     2641 gacccgcgag tatggtcaca cgtcacgcag tccgcttgac tcgctggaga gcgccatcgg
     2701 ctttcacatg cgtgactggt ggcagccgac aaaagcaaac ttcttcggac acctgaaaaa
     2761 gccgcagatt atcgatgccc tgaatgatgc cggactgtcc ggtgctgcgc gggacgcgga
     2821 gaaaatgaag aaaggcgatg cggctgaaca tgcagagttc cacatgaaag acaaccgctg
     2881 ggtgcctggc tggatgtgta caccacgtcc acagatggat gccactgaac acaccactaa
     2941 cctggctgat gccgcctgat gaacaaccac accgccccgc cggagacggg gcggcagcaa
     3001 gggagatacc gtgatgaaaa ctgaactgac cctgaatgcc ctgcagtcca tgaacgcaca
     3061 ggaatatgaa gatatccgtg ctgcgggaag cgatatgcgc cgtaatctca ctcacgaggt
     3121 gatgcgtgaa gtggacgcac cggctaactg gatgatgaat ggcgagtatg gcagtgagtt
     3181 cgggggcttt ttccccgtcc aggttcgttt cacgcctgcc cacgaacgtt tccacctggc
     3241 attatgttcg ccgggagacg tctctcagct ctggatgctg gttctggtga atggtggtgg
     3301 acagcctttc gccgtcgttc aggtgcaaca tatcttcacg cctgtcgcga tcagtcacac
     3361 gctggcgctc gccgcgacac ttgatgcgca gggatacagt gttaacgaca tcattcatat
     3421 tttgatggca gaaggaggtc aggcatgagc gcacgttcac gggcactgat ccccctcagc
     3481 gcagaacaac aggccgccat gcaggctgtg gctgtcaccg aacaacgtcg tcgtcaggga
     3541 cgcacacttt cagcatggcc ttatgcca
//
LOCUS       DAZGBC010000291         3478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGBC010000291 DAZGBC010000000
VERSION     DAZGBC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.293"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(250..816)
                     /locus_tag="ACHK1J_004977"
                     /pseudo
     CDS             complement(250..816)
                     /locus_tag="ACHK1J_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
     gene            881..1549
                     /locus_tag="ACHK1J_004978"
                     /pseudo
     CDS             881..1549
                     /locus_tag="ACHK1J_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
     gene            1964..2845
                     /locus_tag="ACHK1J_004979"
     CDS             1964..2845
                     /locus_tag="ACHK1J_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbaSI family restriction endonuclease"
                     /protein_id="HFO7914112.1"
                     /translation="MDKTDYILRSLTKTSKKRWEHYAVTRIYHLLNDPDIEFVCQQCV
                     RKENGKIYLADLFFPQLELYLEIDEGHHNSEQSQIDDAKRRMDITEVTGFLEKRIPAS
                     EISLEIFDRYINEFIELIRNRKNRMIKDKKFKEWDYDNKYTARPHLKAGRISVGPDAV
                     FRYHRDALNCFGYNKGHHQSGGWSIPSHISEAMGLSGKCMVWFPKLYGNSDWGNELTD
                     DGNKIIERNIKDDEGYTERWDKRIVMAHSRDELNRTLYRFLGVFEAIPGHHTGNILEF
                     HRVSTSVNVYPPNDAAP"
     gene            complement(3156..>3478)
                     /locus_tag="ACHK1J_004980"
     CDS             complement(3156..>3478)
                     /locus_tag="ACHK1J_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7914113.1"
                     /translation="SKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLKAAVRKEFVDD
                     NRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLGVGYSNGADVESPWNAVAGV
                     NWSF"
BASE COUNT          984 a          842 c          804 g          848 t
ORIGIN      
        1 tactgcatac cttaagttta tgtgacatac aaaaaataaa acacccactc cgcgcgcaat
       61 gctctccccg ccacgcctgc ccgcttaaga ggtcgctttt aatgcaggtg catgaaccgg
      121 ctcaggccgc gccggtactg gcgcggtgag gaaaataaaa tacggggatt tgcatgaaaa
      181 accatgcacc ttatggatgc atggcttttt tcggaaaaaa tagcggaatt ttgcggattt
      241 ttttgcgtgc taccgtgcgg ccagttctgc acgtcggcgg gtgtaaatca ggttctgtgc
      301 cggggtgaat ttctggcgat tatcatcccg cgaggccgca tcaggcctga atccgatggc
      361 cgttaaaata tcgctgtcct gcgcggaata attaattttt tcacctgcag ccagccatac
      421 cgacagggct tcacgcagat aatcaaccga gtgctgcatg gcgctctgtt ttactgcggg
      481 aagctgttcg ttatacccca tcagctcagg tgccagcgtg gcggccagct ccgcgccgtg
      541 ctactgcata aagtcatgga gccggttgcg gatgctgatg tgctgcactt cctcatgtga
      601 gcggatataa cgaccggcgg cctgattaat ctcccatttt ttcacgtcga tattgtcgcg
      661 caaatcctgc attctgcgcg ggatttgttc gtcaccggca aggagctgtt cacggtattc
      721 ccgttcaagg tctgccagtt cggctttacg tttcagccag gtatttttgt tcgtctgaca
      781 ggcctcaaag gcctgctgta tggtcacagt ggtcacgagt ctctctcctg attaatgccg
      841 gaacggcgag ctgtagcagc cctgtacttt acgcggcggc ggtgacggcg cagggcttaa
      901 ggccatcacc ggcggcgatg cggtctcggt tgacccgaaa taccagaacc cgtactcaac
      961 acacattccg gcggtgattc tggcagtgaa caataacccc atgcgcttca ccgaccgcag
     1021 cggtggtgtg tcacgtcgcc gggtgattat tcacttcccg gagcaaattg cgccggagga
     1081 acgcgacccg cagctcaggg ataaaattgc gcgcgagctg gccgtgattg tgcgccagct
     1141 catgcagaag ttcagcgacc cgatggcagc ctgcgcactg ctccagtcac agcagaattc
     1201 cgacgaggcg ctcagcatca agcgcgatgc tgaccctacg tttgattttt gcggctatct
     1261 ggaaatgctc ccgcagacca acgggatgtt tatggggaat gccagcatca tcccgcgtaa
     1321 ttatcgtaaa tatctctatc acgcgtatct ggcctatatg gaggctaacg ggtataggaa
     1381 cgtactcagc ctgaaaatgt tcgggctggg gctgcccatg atgctgaaag agtacggcct
     1441 gaattatgag aagcggcaca ctaagcaggg gatacaaacc aatctgtcgc tgaaagagga
     1501 aagctacggc gactggctac cgaaatgcga cgaacccgta gcaacataac ctcactcaga
     1561 ccggcaacag ccggtctttt cctttctggc cattgccaca aggtgaacaa tccactgttc
     1621 acccttcacc gtatcttcac cctgtatcac catgaaatta ttaataaaaa accagaggtg
     1681 aacagtgtga acagtaaaac ctaaaaaaat tttttacccc ccccaccaca tcgcctgacc
     1741 gggcagatcc agaacgagca aaaatcacaa aggggaagat tcgacttttc actcctcacc
     1801 aactcaccac cgcttaacca catgatataa aatagaaaat catcgaagtt aacagtataa
     1861 acagttagat taaaaaaata ctaacgcatc ttatggcgat aggtaacact atcgccataa
     1921 gaaaaagcac attacatgaa gaacaaagaa ggaaggcata taaatggata aaactgatta
     1981 catcctacgt tcacttacga aaacctcaaa aaaacgatgg gaacattatg cagtaactcg
     2041 catttatcat cttttaaatg atcccgacat tgaatttgtc tgccagcaat gtgtcagaaa
     2101 agaaaatgga aaaatctatt tggcagattt atttttccca caactggaac tttatcttga
     2161 aattgatgaa gggcatcaca acagtgagca atcacaaata gatgatgcga aacgtcgtat
     2221 ggatattact gaagttacag gatttttaga gaaaagaatt cccgccagcg aaataagtct
     2281 tgaaattttc gatcgttata tcaatgaatt tattgagttg atacgcaatc gtaaaaatag
     2341 aatgattaaa gataaaaaat tcaaagaatg ggattatgat aataaatata cagctcgtcc
     2401 tcatcttaag gcaggaagga tatctgtagg tccagatgcc gtcttcagat accaccggga
     2461 tgctttgaac tgtttcggtt acaacaaagg acatcatcag tctggcggtt ggagcattcc
     2521 ttctcacata agtgaagcaa tgggtctatc aggaaaatgc atggtttggt ttccaaaact
     2581 atatggaaat tcagattggg gaaatgaatt gactgatgat ggtaacaaaa tcatagagcg
     2641 gaatataaag gatgatgagg gatacacaga acgctgggat aaacgaattg ttatggcaca
     2701 ttctcgtgat gagttgaaca ggacactgta ccgatttctt ggggtgtttg aagctatccc
     2761 tggtcaccat actggcaaca tactagagtt tcatcgtgta agtacatcgg tcaatgtata
     2821 ccctcctaat gatgctgccc catgattaac agcatattta gttccctgtt tatacgagat
     2881 acaacactgc tacagtgaga aaattggtac acgtttaggt acacaactga aagttgaata
     2941 cgaaaaaatc tcttactttt aataggttgg gaaatttatt cagactccgc cagccctcat
     3001 gattggacgg tgtaaggaca acaccaacaa aaacaggaag ttagaagtct cagcaggaca
     3061 ccgaccagac ggtgaggaga cataaaagga tacgcaaagg agccgcggct cctggtgaca
     3121 tgaaagccca cagatgtggg ctttttcgtt gatggtcaga acgaccagtt cacaccagcc
     3181 accgcgttcc acggggattc cacatcggca ccgttgctat accccacccc gagatgcccg
     3241 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
     3301 aaatcattga cgaaattacc gtcattattc accttcaccc ggttatcatc gacaaattct
     3361 ttgcgcacag ccgccttcag ccacggctca acttccatac cgttccccag acgcatgttg
     3421 taactcagcg ttgcccccag ttcacgatat atactgcggg tatcgactga tttcgatt
//
LOCUS       DAZGBC010000292         3464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGBC010000292 DAZGBC010000000
VERSION     DAZGBC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.294"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..462
                     /locus_tag="ACHK1J_004981"
     CDS             136..462
                     /locus_tag="ACHK1J_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO7914114.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(594..794)
                     /locus_tag="ACHK1J_004982"
     CDS             complement(594..794)
                     /locus_tag="ACHK1J_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7914115.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            836..1201
                     /locus_tag="ACHK1J_004983"
     CDS             836..1201
                     /locus_tag="ACHK1J_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7914116.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            1484..2041
                     /locus_tag="ACHK1J_004984"
     CDS             1484..2041
                     /locus_tag="ACHK1J_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7914117.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            2038..>3464
                     /locus_tag="ACHK1J_004985"
     CDS             2038..>3464
                     /locus_tag="ACHK1J_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7914118.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQSWKLGGA"
BASE COUNT          788 a          803 c         1040 g          833 t
ORIGIN      
        1 gctaaatgcc gcatggtatc cgctgcatta cagccagtgt gttgagcgac cttattatcc
       61 atgcgcggta ttgtgatggc cgacagggtg gcattgttag aataagactt attcttatct
      121 gtgccgggaa tgaaaatgaa aagaaatctt ccgttaatta ttttgttgtc ttctctggtt
      181 atgggctgta cgcaacataa aacagatatg ccccgacagt tggttaaggc attaccacaa
      241 tatccggcct atgcagcggc aaattatata aagggacggg ttgatgtgaa gtttgatatt
      301 ggtgctgatg gtactgtcac ccgaattgag tttatccgtt cagagccgca ccatctgttt
      361 gatgagcagg ttgtaaaggc gatggcaaaa tggcgatttg agaaggacag gccgcgtaaa
      421 ggcgtgaaga aaacgtttat ctttagtcct tctgcaccct gattatttca tcagaaatta
      481 attatcactc tgttgttatt ctgtacatcc cggctgggta agtcttgttc cgccgggtat
      541 gaagatgaaa tattgttgga ggacagtggg tacctgctcc tgtaaccgaa cgttcatttc
      601 tcgttatttg tcatgctggc cgggcgcagg tgcgttgcat ctgttgccag ccttctccgg
      661 caggcttcaa taacccacgc tgaaaagtta ccggaccctt tatgctcaag ggcgatgttg
      721 atctgttcaa tcatgtgatt ggggaaacgg atattgcggg ttgtggttct gcgggtccgg
      781 tttttcgatg acatatttat ttcctttact gattgcctta tgacggggat tttacatggc
      841 tgagcttcgt acactccaga gcagaatcaa aacactgaat acccgacggg tgaatattct
      901 gaagggtgaa cagcgtcgtg tcagtggcag tgcacgtgtt tccctcaagc gtcatatctg
      961 gctcagggat gccgggcagt gctgtctctg tggtcgtgtg gttgacctct gtgacagtga
     1021 actcgatcac cgaattgcac ttcagttcgg tggtggtaat gaggagacga atctctggac
     1081 gctctgtacc gaatgccatc gacaaaagtc tgctcgtgaa gcggcgggtg gtatgccgga
     1141 cccgacactg ccggaggtgt ccggaggtag tggcagagcg gacgacatca tcggactgta
     1201 acccgacccg gggggtatca tccggcgtaa aaaacgatcg ctccggacac cgcgccccct
     1261 ctcacgcaga gaaaaaattc cccttcagaa gttataaaca tgtttataac ctactgatgg
     1321 atttttaccg tttttttctt tttattcata acgttgtggg aggtaaatgt ttataccttt
     1381 ttttcgcaaa atgtttacag ggcagcggta aacattttcc ctgccgcctc cattcttacc
     1441 gggaggattg ctgacgtttt tctgtttttc cccggaggaa agaatgctga cgacgcaaaa
     1501 aagaaaattt gcgctggcgc tcatgtcggg caaaaacaga acggctgctg ctattgaggc
     1561 tggttactcg gaaaagtccg ccagatgtaa aggctcacaa ctggcaaaag atccggaagt
     1621 gctcgcgttt atagcccgta agcagggcga gacggtggag gtggatgagg ttcctgttta
     1681 ccggcagaaa aagacgcagg cgtgccaccg ggaagttcct ccggtggaaa agaaaaacca
     1741 ggtttcagtg ccaccggatg acgggcaaag tttctctcca ctatccggta ttgattacat
     1801 ggaggacggc cttcctgatc ctgtgaaagc catgggtcgg atcctggtgg aaaacatcag
     1861 cattgatccg aaactggctc tggatgcagc ctggcgactg gcgcagttca cgcaccacaa
     1921 aaaaggggat gccggtaaaa aatcggcaaa aggtgacgcg gcgaaaaaag cggctaaccg
     1981 ttttgcggtg ccaccaccac cccgcctggt ggtgaataat gataatgagg gcaacggatg
     2041 atacctgtgt ggagcacggc ctgcccggac tgggcagagc gtctgaaaaa ggggctgtcg
     2101 attattccgg ctccgattta cccggaccag gccgcacatg ccctggcgat ttttaaacaa
     2161 ctgcggattg tggatgcacc gggcagcccg acgttcgggg agtcctgtgc accgtgggtg
     2221 tttgatctgg tggctgccct gtttggctcc tacgatgcgc agaccggtgt acgccatata
     2281 aaggaagtgt tcattctgat acccaaaaaa aacagcaagt ccacgctggc tgccgggatc
     2341 atgatgacgg cgctgttact gaactggcgg caggcggcgg gctacaccat tctggccccg
     2401 accgtggagg tggcggctaa cgccttcaac cctgccaggg atatggtacg acgggacgat
     2461 gatctggatg acctctgtca ggtgcagaca catatccgga ccatcaccca tcgggtgacg
     2521 gacaccaccc ttaaggtggt ggctgccgat ccgaatacgg tgtccggtat caagtccgtg
     2581 gggacgctga ttgatgaact gtggttattt ggcaagcagt acaaagcgga ggacatgtta
     2641 cgtgaagcca tcggcggcct tgcctcccgc ccggaagggt ttgtggtgta cacaaccacc
     2701 cagtcgaatg agccgcccgc cggggtgttc agacagaaac tgcagtacgc ccgggatgtg
     2761 cgcgacggca aaattaatga tccgcacttt ctgccggtga tatttgagca tccgccggag
     2821 atggtggagc gcggtgagca cctgctgatg gaaaacctcg ccatggttaa cccgaatctc
     2881 ggttattcgg tggatgaggc ttttctgtac cgggagtacc gtaaagcccg ggaggctggt
     2941 gaggaggcat ttcgtggctt catgtcaaaa cacgccaatg tggaaattgg tcttgccctg
     3001 cgttctgacc gctgggcggg tgcggatttc tgggagcagc agggcaggcg cgtcagcctg
     3061 gacgatatcc tgcagcgcgc tgatgtggtg acggtgggga ttgacggcgg gggcctggat
     3121 gatctgctgg gaatgtacgt gattggtcgt gacagggaaa cccgcgaatg gctgggctgg
     3181 ggccatgcct gggcgcatga aaccgcggtg gtcagacgga agagtgaggc atcccggttt
     3241 caggattttg tggccagtgg agacatgacg attgtccgtc gggtcgggga tgacacggcg
     3301 gaagtggcgg agtatgtgcg tcgtattcat gaggctgagt tactggacca tatcggtatt
     3361 gacccgtcag gtgtggggca gattctggat tcactggcag aagccgggat ccccgacgga
     3421 attgttgtgg ggataagcca gagctggaag ctgggcgggg ccat
//
LOCUS       DAZGBC010000293         3417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGBC010000293 DAZGBC010000000
VERSION     DAZGBC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.295"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..269
                     /locus_tag="ACHK1J_004986"
     CDS             <1..269
                     /locus_tag="ACHK1J_004986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914119.1"
                     /translation="SEKFTEYKKDLFLTDCYFLKIFSAPFAFRVRRMAGYGMVGLQPQ
                     GDDCNTSDHHSQSSVNVKYLTVQSVDYCRSYGIFVKKILHGESP"
     gene            539..766
                     /locus_tag="ACHK1J_004987"
     CDS             539..766
                     /locus_tag="ACHK1J_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFO7914120.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            1144..1719
                     /gene="dolP"
                     /locus_tag="ACHK1J_004988"
     CDS             1144..1719
                     /gene="dolP"
                     /locus_tag="ACHK1J_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7914121.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRLSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSCIR"
     gene            complement(2149..>3417)
                     /locus_tag="ACHK1J_004989"
     CDS             complement(2149..>3417)
                     /locus_tag="ACHK1J_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7914122.1"
                     /translation="ISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAKANF
                     AVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRGAGI
                     EAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKIISAYNSQGTLSAEETKQLEEVKKE
                     RADMFATYMATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQTVT
                     EKADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKL
                     LISENSKQISTNTLGIRENTQRIRDEGQARIRGDRETLRAATGYTDMRVNGLERNTNK
                     RFAQLKEDIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHF
                     NTSENSAVKVNAAWDTENGAGVSAGMALGW"
BASE COUNT          890 a          775 c          772 g          980 t
ORIGIN      
        1 cttctgaaaa atttacggaa tacaaaaaag atctgttttt aactgattgt tactttctga
       61 agatattctc tgctccattt gctttccggg taagacgtat ggctggttat ggtatggtcg
      121 gactgcagcc acagggggat gactgtaata cctctgatca ccacagtcag tcatctgtaa
      181 atgttaaata cctcacagtt cagtcagtgg attattgtcg gtcttacggg atatttgtta
      241 aaaaaatact gcatggtgaa tccccctgag cggcggggca tatcagtatc gtaggtgttt
      301 ggtcacatcc cttatctgtg cgggttcagg tgctgatact gaactcaccg ggaggcaccc
      361 ggcaccatgc tacataacac tccattacgc tacactcgcc cctctccgga ggggttttct
      421 ctgcaggatg tgaatatgag atgctgcacc attcactgcg aatattgatg gtgagaatct
      481 ttaataaatt taaagtttat atgtactgtt acagtgttga tgaaaggaga taaaaacaat
      541 gttcccggag tacagagaac tgatttcagc attgaaagga aatcataaac gctttgattc
      601 tttatttgaa aagcataata aacttgatca cgaaataaaa agaattgaag atgaatttgg
      661 ttcagcgtat tcgacgaaag tggtcaggct gaagcttgaa aaacttgaga tcaaaagaga
      721 aatccagaaa atccttgaag atgagtcaga aaagggcgaa cggtaatcat aaacggtata
      781 ttcaaagacg tgttttctct gttttatgaa aaaggcaccg ggggctccac ggtgcctttt
      841 agcctgtgta taacgaaaag gagatcctgt tcagcgaacc agatagttaa aaaaagaatc
      901 tttacgacgg tatcaaagtt ttcgtgcaaa aaacatgatc aatattaaat tccagtacat
      961 ccatatccga tataaatctt tccgtctgac agatacagat atgccacgta acgcatccgc
     1021 tgtttccggc tgtgccggag gataataaca gtctgtagct tgttttgttt cactgaaggt
     1081 aaactttttg tacgataaat tccgggaata tgaagtaaaa atcaaaacaa ttccggggga
     1141 gatatgcgtt tattatattc tgctgttgtc ctgatttcag ccttattgtt acagggatgc
     1201 gttgctgcaa ctgtggtggg tacagccgct gtcgggacaa aggcagcaac cgacccacgc
     1261 agtgtcggaa cgcaggtgga tgatgaagtg ctggagctga ggctcagcag tgcactttca
     1321 aaagatgaac aaatcagaaa agaagcacgc atcagtgaga ttgcatatca gggacgcgtg
     1381 ctgctgatcg gtcagtctcc ggaccgtgaa ttatctgccc gtgcccgtca gatagcactc
     1441 ggtgtcgatg gtgttcagga ggtctataat gaaatccggc aggggacacc cgtcagtccg
     1501 gtcataatgg caggtgatgc ctggcttacg acaaaaattc gttctcagtt actcacaagt
     1561 gcccgtgtca aatcatccag tgtcaaggtg gtgacagaaa acagcgaagt ctttttactg
     1621 ggcctggtta cagagcagga agggcgagcg gcagcagatg ttgccagtag ggtgagcgga
     1681 gtgaagcggg tcacgacggc cttctcctgt atacggtgaa ggcagtatca gagttgcctt
     1741 tagtgtaaat ttacgttaaa aatcagaaga gcacgtttcc gggccgttca tgccaccacg
     1801 tgaacattcg tcgcggaata ccggaggcgt gctcattata ataaccaata tacataattc
     1861 ctctttgaca cccccctgaa ttcgggggat ttttttgccc gggtatgttc actcacagat
     1921 aaaatcccgg tgcgctccgg atgtcgctgc tggtaaataa tctgagcttt tatccataca
     1981 ggtcaaagag cgtggcgttg acacgtgtac gtctggctcc ggtgcaggag ccagattgtc
     2041 cgtattaatt acatcctgag ccgatgcgat aaactgaatc gcttcctgaa tggtggtgca
     2101 gtgtcccctg gataacaata tctgcgaacg gcttctggcc cggaagcatt accatcccag
     2161 agccatcccg gctgaaacgc cagctccatt ttcagtgtcc caggctgcat tcactttaac
     2221 agcactgttt tcacttgtat taaaatggaa gccggcagca acagcacctt cactccggta
     2281 tccggccatg gccataccaa atgagacctt tttcccgggt acggacggta tccccgtcat
     2341 tgccatcgca cctgcaatac ctgcatctgc acgttttctg tttttgtcta tatcctcttt
     2401 aagctgcgca aaacgtttat tcgtattacg ctccagcccg tttacccgca tgtcggtata
     2461 accggtcgct gcacgcagtg tttccctgtc tccccggatt cgagcctgtc cttcatcccg
     2521 gatccgttgt gtattctctc tgataccaag agtgttggta ctgatttgtt tgctgttctc
     2581 cgaaataagc agcttatgtt cttctgctgt ctgtgtattg gctgtcactc tttcatccag
     2641 aatttttgtc gttttattaa tgttactgat ttcctcttta ttactgttaa ccacctgcag
     2701 atttttagtt atcgcactgg tgttgtttgt tatgcgttta tcaagcgctg catctgcctt
     2761 ttctgtcaca gtctgcaaat ccctgagctg tgcaacgtta acagcatccg tatcgacaga
     2821 acctgctgca acgctagtta tctgtcttgt aacagcatgt cgtcctgttg tcgttgcacc
     2881 aacacctaca gatacagcat cgtgcgttgc catatatgtc gcaaacatat cagcccgctc
     2941 ttttttcact tcctccagtt gtttagtctc ttcagcagac agcgtccctt gtgaattata
     3001 agcggatatt atcttatcga tttccataag cctgttaagc tgcttgtcag acatcctgga
     3061 ttttactaca tcccctggat tccagcgctt tgtctctggc atatatgctt caataccagc
     3121 gccacgatca gcaacacttc ctgcgcccag agcgacagag aatggtttca atgttgttgc
     3181 gtttgctcct aacgcggtag agttattttc aatagctctt gctgatgcac ctaaagaaat
     3241 acttttttct gcatttgcct gtgaatatcg accaacagca acagcgaaat tagcttttgc
     3301 cgcactgtta tcaccaagtt ccacgatcat tttatcagga ataccatttg cagataagac
     3361 aggtaaagaa gctgagcagt agagaaataa tgatttgcat atataactga cactgat
//
LOCUS       DAZGBC010000294         3392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGBC010000294 DAZGBC010000000
VERSION     DAZGBC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.296"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1399)
                     /locus_tag="ACHK1J_004990"
     CDS             complement(<1..1399)
                     /locus_tag="ACHK1J_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914123.1"
                     /translation="MRMFTNLLYDICTVFELFKEGESPRDKRKSTDFGAHQRFWDQRY
                     NELSHIIDAEGVYSLEQRRIIFSRYEYFYYMMNSYPVYSTLKSEYIRNYFLKSFGVVF
                     IVLDIYNTYRPENETGFYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGF
                     NREDFRENGKMYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELD
                     RILNNIDKFYYAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLY
                     DFNIFDTTPYARALLERFHEFESGPKGALTRYIVSLDDKSQEYIESLKDMVFNLSDKK
                     SYDDVYLENIINYFEQLQNARGYVTRCYMLLAVFIYLIRRNKLHKALRFYDESQKYEL
                     PFGYLPGAFSVLRIALEIKVNREKIKHGSLFELLDYVKAYQDAFMDLRVVTDPAYNED
                     EIQYDANNFTLMRVIK"
     gene            1500..1748
                     /locus_tag="ACHK1J_004991"
     CDS             1500..1748
                     /locus_tag="ACHK1J_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7914124.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            1764..3329
                     /locus_tag="ACHK1J_004992"
     CDS             1764..3329
                     /locus_tag="ACHK1J_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO7914125.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDEAL
                     DILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFMVCWDHKLNKYRN
                     EEQTKIWNITTKN"
BASE COUNT         1088 a          754 c          625 g          925 t
ORIGIN      
        1 ttttaatgac acgcatcaga gtaaaattat tcgcatcata ttgtatttca tcttcattat
       61 acgcaggatc cgtcacgacc cttaagtcca taaatgcatc ctgataagct tttacataat
      121 caagtaattc gaacagtgaa ccatgcttta ttttctccct gtttactttg atctccagcg
      181 ctatcctgag aactgaaaaa gcgccgggaa gatacccaaa aggtaactcg tatttttggg
      241 attcatcata aaaccgaaga gctttgtgaa gtttgttccg ccgaatcaga taaataaaaa
      301 cggccagtaa catgtagcac ctggtcacat aaccacgggc attctgcaac tgttcaaaat
      361 aattaataat gttttccaga tatacatcat cataactttt cttatcagaa agattaaaca
      421 ccatatcttt taaagattcg atatattctt gtgatttatc atcaagtgac acaatataac
      481 gagttaatgc tcctttgggg ccactctcaa actcatgaaa ccgttcaagt aatgctcttg
      541 cataaggcgt tgtgtcaaaa atattaaaat cataaagata gcgtgcagcg taaccataca
      601 acccatgtac ccaaagccct tctcttatca gcactctgag ataatatgca atactgtcaa
      661 gtatctttac cttccgttgc atatcaagca aaatacttaa tgaataaaat gcataataga
      721 acttgtcaat gttgttgagt attctgtcca gctcactctt atcaagcttc ttctctctgt
      781 aatccttctt gtattcattc ttacaggcct ttatatattg tttcataagg ctcttccttt
      841 taccgtaccc ttttcgaata gttccctggt attttcccag ctcatacatt tttccgtttt
      901 cccgaaaatc ttctctgtta aatcccagtt cagcaagata ctcccggaga taacgtttaa
      961 cggcagcttc atcgctttct gtaccagagt aatccaggca gggacaatat gatttttgca
     1021 aaaaattata gatatgataa taaaaccctg tttcattctc cggacgatat gtgttatata
     1081 tatccagcac aataaaaaca actccaaagc ttttcagaaa atagttacgg atatattcgg
     1141 actttaatgt tgaatacaca gggtaagaat tcatcatgta ataaaaatat tcataccggg
     1201 aaaatataat tcgccgttgt tccagactat acaccccttc agcatcaata atgtgactga
     1261 gctcattata acgttgatcc cagaatcttt gatgcgctcc aaaatcagta ctcttccgct
     1321 tatctctcgg actctctcct tccttaaaca actcaaaaac tgtacaaata tcatacaaaa
     1381 gatttgtaaa catgcgcatc tgctctgatc tccggttggt ttccgtttta tcacagggga
     1441 cattctggcc ttaacctagc aaattaatga catagccttc aactcacatg gagaacaata
     1501 tgtcaaccca gaccggacat ttttataccc cctcacgcat cctgcgtatg gctgaactta
     1561 cccggctcct tggtatatca agatcgacca tctacgaaaa aatgaatccg gcatcaaaat
     1621 actacgatgc cacctttccc cggcctgtcc ggcttgggag ttcggctgta ggctggcggg
     1681 catctgccat tgatgagtgg ttaactttac atacaacacc aaccagatcc gtaactaagg
     1741 ataaataagg aaaactgtac gccatgacaa aattatcccc ccctgaactt gacataacta
     1801 aactgcctga caggtgccag gcattacttg atgaaatgca tgaagaaacg ggaataagcc
     1861 gtgaaatact gctgtctatt atgctgacgg tcaaggctgc ctcggtccag gatacgcatg
     1921 aagttgaact ttctggaggg cagcgtacca gtcttcagat atatatgtgt ctttcatcag
     1981 catcaggcag tggaaaaacc tctgcctgca caaaattaat cgcccctgtt catgaaacag
     2041 aagaagaact acatcaggca tatatcgacg ataaaaaaaa ctatgatcgc atgatggaaa
     2101 tgtggacaac agataaaaaa atcctggaac ggagatataa aaaagaaatg gaaagatccc
     2161 cggaaaatgc tgctgctgca cgagcagcgc ttgaaaagtg tatagcaaat aaacccgtac
     2221 ctcccgtaca acaggttctc atcgtgaatg acgcgacacc agaagggata gctttgaaac
     2281 tcagccagtc accttctctt ctgttgctgt ctgatgaagg aggaactatt ctggataagc
     2341 gtttcgaacg caaatcggcg ctgtacaaca cattatggag tgggcaacca gtcaccgtag
     2401 aacgggcatc cagacccggg ttccgggtaa aggattcccg tctcactatg cttatactca
     2461 ctcaaccggt aatatttgat aagtttttta ctctcactgg cgaccagatc cggggcaatg
     2521 gctttctggc ccgggtgcta ttttgcgaac ccggagcaaa taaaataatg acgacagaac
     2581 cgcatactgc cactcctgtt gtaacgcagc aatgtgcaac ctgttttcat gagaaaagtt
     2641 ttggcagtca gatcagagac tctctgagag cgtcccggga aaggcgtgca aaaggtgagc
     2701 aacgcatctg catgacatta tcaaacgcag catcccacga tctggaaatc tttcacaaag
     2761 aaaatatgag tgcagtcagg cagaacccac gtatgaccac tttcgaagac attatcgtca
     2821 ggaaaagaga acaggctgtt cgaatagccg cacttctgga gctggagaac aatccacatg
     2881 gtacagtaat aacacgagaa agtatcaata gtgctattta tctcgttgat ttttattttc
     2941 agcatcttat atccaaactg gaatcacttc gggaaatatc tcctgcagaa aaactggata
     3001 aatggctcag agaaaatatc atccgggtaa aaggatacga atatcaaaaa agccatatac
     3061 tacaatatgg tccatatgcg ctcaggaata aatgtgttct tgatgaagca cttgatatac
     3121 ttgcagaaca gaaaaaaatc gtaattgatt attcaatcgg ccaaaaaatc atttatatcg
     3181 gggacgcgat tacaccttgt gaattagcaa atgaggcaaa catcccgata atggagcgtg
     3241 gaatgtttat ggtctgttgg gaccataagc tgaataagta cagaaatgaa gaacagacta
     3301 aaatatggaa cataactaca aaaaattaaa gttcccgtct tttatcattc agttaccaac
     3361 ctgaatatct gaaacaatgc cagacatcac tt
//
LOCUS       DAZGBC010000295         3356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGBC010000295 DAZGBC010000000
VERSION     DAZGBC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.297"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..230
                     /locus_tag="ACHK1J_004993"
     CDS             <1..230
                     /locus_tag="ACHK1J_004993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914126.1"
                     /translation="KNNTLKPLSRQYSSRFLTDFLNKKTSFAGGWSHCVTPTTITSVF
                     SRAVVNHKIGTHAGGAGPHDFLFDESENGER"
     gene            521..715
                     /locus_tag="ACHK1J_004994"
     CDS             521..715
                     /locus_tag="ACHK1J_004994"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7914127.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIEAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            1145..1372
                     /locus_tag="ACHK1J_004995"
     CDS             1145..1372
                     /locus_tag="ACHK1J_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914128.1"
                     /translation="MSIDVIKQIQSIQDKGIIIYSKYRANEFNRDDVYRESYSLIIEF
                     NHLIAEHAVHDEKLINHTVCILNELRSIAIN"
     gene            complement(2039..3037)
                     /locus_tag="ACHK1J_004996"
     CDS             complement(2039..3037)
                     /locus_tag="ACHK1J_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO7914129.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFQRLKDRKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVAETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
     gene            complement(3124..>3356)
                     /locus_tag="ACHK1J_004997"
     CDS             complement(3124..>3356)
                     /locus_tag="ACHK1J_004997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914130.1"
                     /translation="ARKRSKNAHFGWVIVRAILVFILKGYLWDCNLALALVASGLSAD
                     LLGNGFESILSEVVALKATPATTGIVRGCDHL"
BASE COUNT          935 a          842 c          754 g          825 t
ORIGIN      
        1 aaaaaaacaa cacactgaaa ccactctccc gacaatactc cagcaggttt ctcaccgact
       61 ttctgaacaa aaaaaccagc tttgcaggtg gttggtccca ctgcgtgaca ccaaccacca
      121 tcaccagtgt tttttctcgt gccgtcgtca atcacaaaat cggaacccac gccggaggcg
      181 cgggacccca cgattttctg tttgacgagt cagaaaacgg cgaacgctga cagatttcaa
      241 tctggctgcg tgagacgttg gcggggtgtc ggggcgcagc cctgaccagg cggcatttgt
      301 ctgattgcgt gtgcgcggtc cgacaaatac acatcctgtc ccgtttttcg cacggttttt
      361 attgattacc ggacagatga atccggattg ccatgttgcg aaaatcacgt taaaacgatt
      421 acgagcgtgc agttgggctg atatatcaac agtcatgcca atatgtatta acccgacagg
      481 ccgctctttt ttaattcaaa taaaaatata aattacgagt atgaatcagg acaaagcaat
      541 ttatttagaa aataaatatt tttcacccgc aaacgaagct gtcagactgg agcgtgaagg
      601 agattacatt gaagcatgga aaaaatggaa tgatgcgctt cattatgcca aaggagaaaa
      661 tataggttgg tgtatagcca gagctgaata ttgtaaaaag acaggccggt tttaattctg
      721 gcggggtttc aaaggggcgt cccctttggc acgtcgacgt atcaaaatgc gccagcattt
      781 tgtatacagt cggtcacgtg cctccaggat tgccccataa tatttcctat ctacataatg
      841 tctaactaag atccatctaa tatccatcta agatccattt aaggtccatc taaatcagta
      901 aatatctaac tagtgtccat ctaaggtcta tctaacgtcc atctaaaagc taactaacgg
      961 ttcaccaacg gatcgcatgt agtagacgcc acataaaaaa tcctgaaatg gaggaatcgt
     1021 cataaagaga ttcctgtttt tagcgttctg atgcaggata gggcaaacgc cgcaaaatga
     1081 cgtctttgat atattttgtg atgtttgttt cgggcatctg ttatctgttt tattgggtgt
     1141 ttttatgagt attgatgtga ttaaacaaat tcagagcata caggacaaag gaattattat
     1201 ttattcaaaa tacagagcaa atgaatttaa ccgggatgat gtatacaggg agagttacag
     1261 cctgattatt gagtttaatc atcttattgc tgaacatgcc gttcatgatg aaaaactgat
     1321 taatcatact gtctgtattc tgaatgaatt acgaagcata gcgataaatt aaaaaatccc
     1381 cctggattac agggggaagc catcagagtt tgagtaatga gaaatattat tttggagaga
     1441 gcaccacaaa tgcgctcttt tccaatctgc cgtaaccatc agcaaaagac aatcaggaca
     1501 ggcgaaaaaa tccccctata tggcaagggg gtgtcaacga ggacatggca attgtgagtt
     1561 ttgtgtgttt tatgtgttgc agtttgcaac gtcagaaatt tgccgcagtt atgcgcgaaa
     1621 aacaatcctg atttcgggac aggatgtgta atcgtataac cgcgcacgca cggctatacg
     1681 attaccacct ggtcagggct ttgcccgaca ccctcgacac cgttcccgca atacgataga
     1741 acatcagggc gcgtttccgg gtaaacgcat gttaatgacc gggaattcat tgattaggga
     1801 ccggagacgc gctacaaaac tcctgcgcgt ttctggcatc aactgaccat ggggatggtt
     1861 tggcaatagc agcaagcgcc agaaacgcgc tcaaaacgtc tctgacggcc tgaatcaacc
     1921 aaaacatata aaaccattgc ctgatgcaaa aaatcgcgtt agaaagcgtc tgagtgcgtt
     1981 tctggtgagc aaaaagctgt cagccgtgga ttgccattgc agagacccag aaacgcgctc
     2041 aatcatcgct gcttagccga cgtttatctt tcgcccgatc gcgctggtat tttttgacaa
     2101 ccggcaccca cgaaaacgcc agacgggaac cgtcatctgt accttctgcc atgccatccg
     2161 ccatgattaa atcctcgttc gtttcccgct ccagttgcag aacgttctta agcgctcccc
     2221 ccgtggcaac aaaacgacgc ttgtgtacct gccgtgttaa ttcgaggaac cattcaggat
     2281 cagccagcat atcagcaggc tttgtggaat atttcagagt ttcagccacc gcgccgcgca
     2341 gctgttccgc aacaacacaa ggtaaagatt catcgtcttt tggtttccgt gacttcacca
     2401 ctctgatatc cacattcggg tcatacgcca cccgcagaca atcacgccac agttccaccc
     2461 aacgggcatg tttcacatac tcacggccgg acaacatcga ggggggaacc atcagcagag
     2521 tgtgaaaatg gggatgtgcg ctaccatcct tgccgcgcgt tacttccgtg gtacgtaccc
     2581 atcccagcac atgacgaaat tccttgcgat ctttcagccg ctgaaaacca gcattcatcg
     2641 cggtcagcgt ctcaccaagc gcgtcaatgg aacagttgcg cactgtcagc gtgagaaaca
     2701 accagcgcgc gccgggatgt tccgtcacaa tctttggcag ggactgatag aaacgggctt
     2761 gccacatcat ggagcgcctc cactggcaga tcggacagtg ccggacacga cagaaccgcg
     2821 catcacgcaa ccgcaggcgc gtttctccgg tctctgtgtt tgttgaccac ccaaaccgca
     2881 aaataccact acaagccccg acacgttcgc cgtagtgctc aaatccggca gcacgcagat
     2941 aaataccgcc cacatcgtcg gaaaagctcc ggcgctcatc ccacggttta tcctccgggg
     3001 aataatccgt cagattttta agttcaggat ttttcatgtc tgccgttccc tagtgagcga
     3061 cagacgaaat tacgtgttat cattcctgta tcacctgtgt tgtcgtgtca ccaattcgaa
     3121 ttcctacagg tggtcacacc cccgaacgat gccagtcgtt gcgggggtcg cttttaatgc
     3181 gaccacttcc gacaaaatac tctcaaaacc atttccgagc aaatctgcgc tcagcccaga
     3241 tgcgacaagg gctagagcta aattacaatc ccagagatat cccttaagta taaatacaag
     3301 gatcgcgcgc acgatcaccc acccaaaatg cgcgtttttt gagcgtttcc gagcac
//
LOCUS       DAZGBC010000296         3353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGBC010000296 DAZGBC010000000
VERSION     DAZGBC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.298"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..186
                     /locus_tag="ACHK1J_004998"
     CDS             <1..186
                     /locus_tag="ACHK1J_004998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914131.1"
                     /translation="NTHPVPFFARFLLITGQMNPDCHVAKITLKRLRACSWADISTVM
                     PICINPTGRSFLIQIKI"
     gene            196..390
                     /locus_tag="ACHK1J_004999"
     CDS             196..390
                     /locus_tag="ACHK1J_004999"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7914132.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(1946..2962)
                     /locus_tag="ACHK1J_005000"
     CDS             complement(1946..2962)
                     /locus_tag="ACHK1J_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO7914133.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLAERPAARWLFMTLTVKNPPVEDTRETLIRMNAAWNRLVRRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLIGADIPAELDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
     gene            complement(3153..>3353)
                     /locus_tag="ACHK1J_005001"
     CDS             complement(3153..>3353)
                     /locus_tag="ACHK1J_005001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914134.1"
                     /translation="VLLFRKSVRNLLIRWFGCVVFLFSGTLKKRAFWVGDRARDPCIY
                     TIGNNSRMCYFGLAQWWRGFER"
BASE COUNT          968 a          843 c          698 g          844 t
ORIGIN      
        1 aatacacatc ctgtcccgtt tttcgcacgg tttttattga ttaccggaca gatgaacccg
       61 gattgccatg ttgcgaaaat cacgttaaaa cgattacgag cgtgcagttg ggctgatata
      121 tcaacagtca tgccaatatg tattaacccg acaggccgct cttttttaat tcaaataaaa
      181 atataaatta cgagtatgaa tcaggacaaa gcaatttatt tagaaaataa atatttttca
      241 cccgcaaacg aagctgtcag actggagcgt gaaggcgatt acattggagc atggaaaaaa
      301 tggaatgatg cgcttcatta tgccaaagga gaaaatatag actggtgtat agccagagct
      361 gaatattgta aaaagacagg ccggttttaa ttctgacggg gtttcaaagg ggcgtcccct
      421 ttggcacgtc gacgtatcaa aatgcgccag cattttgtat acagtcggta acgtgcctcc
      481 aggatttacc atcaaaaacg cccctgtagt taatcctgaa tcaagatata actaagatat
      541 aattagcgta taactaaggc ataactaaag aataactaac ttagtgtgca tataattaag
      601 atataactaa tatataacta atgtataact aacggttcac caacggatcg catgtagtag
      661 acgctacata aaaaatcctg aaatggagga ttcgtcataa agagattcct gtttttagcg
      721 ttctgatgca ggatagggca aacaccgcaa aatgacgtct ttggtatgtt ttgtgatgtt
      781 tgcttctggc atctgttatc tgttttattg ggtgttttta tgagtgttga tgtaattaaa
      841 caaattcaga gtatacagga caaaggaatt attatttatt caaaatacag agctaacgaa
      901 tttaacaggg atgatgtata cagagagagt tacagcctga ttattgagtt taatcatctt
      961 attgctgaac atgccgttca tgatgaaaaa ctgattaacc atactgtctg tattctgaat
     1021 gaattacgaa gcatagcgat aaattaaaaa atccccctgg attacagggg gaagccatca
     1081 gagtttgagt aatgagaaat attattttgg agagagcacc acaaatgcgc tcttttccaa
     1141 tctgccgcaa ccatcagcaa aagacaatca gaacaggcga aaaaatcccc ctatatggca
     1201 agggggtgtc aatgaggaca tggcaattgt gagttttgtg tgttttatgt gttgcagttt
     1261 gcaacgtcag aaatttgccg cagttatgcg cgaaaaacaa tcctgattcc gggacaggat
     1321 gtgtaatcgt ataaccgcgc acgcacggct atacgattac cacctggtca gggctttgcc
     1381 cgacaccctc gacaccgttc ccgcaatacg atagaacatc agggcgcgtt tccgggtaaa
     1441 cgcatgttaa tgaccgggaa ttcattgatt agggaccgga gacgcgctac aaaactcctg
     1501 cgcgtttctg gcatcaactg accatgggga tggtttggca gtagcagcaa gcgccagaaa
     1561 cgcgctcaaa atcatcagat gtacagcgaa caaaaaattg tcatctgcaa aaaatcacgt
     1621 taaaacaatc gcgagcacct tcctgtctcc ttcaattacg gtcaaacatt ttgcatctgt
     1681 cgggaagttg ctcacctcca taattcaatt tctcattgac aataattccg gtgatatccc
     1741 ctcttttgag gggctttttt tacccacaca ccacaaacag cagccatacc atcgtcaaaa
     1801 acgcgctcaa aacgtctctg acgccctgaa ccaaccaaaa catacaaaac cattgcccaa
     1861 tgcaaaaaag tgcgttagaa agcgtctgag cgcgtttctg acgtcttttt tgttgctgtg
     1921 tcgctactcg ttgtcagatt cgcgctcaat ccttcatcct gtcagcttca ggattccgct
     1981 tgtaacgttt ttccgtacgt ccccagtcaa acgccagcca gaaggcttgc tcctcgaact
     2041 cgtccagttc tgccggaata tctgccccga tcaaatcctc gtccgtctcc ggtttatcga
     2101 ctttcagaac ctctttgaga cgaccgccgg ttgccaccat gcgaagccca taggtctgcc
     2161 gcgccagctc cagaaaccac gccggatcac gcacaacttc cgaagatttc accgtgtatt
     2221 tcatcgtctc aatgacgcct gactccagcg ccgcacgatg catatcaggc agggttacac
     2281 catccggccg tttccagccc tgtttcggtt ttaccacccg aatatccaca cccgcctcat
     2341 aatcgacacg aaggcattca ctccagatct ccacccagcg agcatgtttc acataaccat
     2401 taccggacaa catcgaaggg gggaccatca tcagaacgtg aaaatgcgga tggcaacatc
     2461 ctggcacctt cccgtaagta atttccgtcg tacgtaacca gccaagaact gacctgaatt
     2521 ctttacgccg aaccaacctg ttccacgccg cattcatccg aatgagtgtt tcgcgggtat
     2581 cttccacagg gggatttttg acagtcagtg tcatgaacaa ccagcgggcc gcaggacgtt
     2641 cggccagcag ctttggcaag gcctcccaga ggcgacgcat ccagcggaga gagcgacgac
     2701 gactacacac cggacaaaac ggaacatgac aaaactcccc cttccgaagg gccagcttca
     2761 tttcgcccgt caggcgggac accttcggcg caaacaacaa ccgacgggaa caatcaaaca
     2821 tccgttctcc acgagaggaa aacatcgaat cgctttcgta aatctgcgcc accctgtcag
     2881 ccatcactcg atcactgtcc cattttgtat cccgcgtgct gtaatcgcgt aacgagagta
     2941 ccggactgtc tggatttttc atttgcaccc cccaagaact gggcagatgc ggaaattttg
     3001 tgttagcatt tttctgtcac ctgtgactct gttgccgaac aacgaattcc aaactggtga
     3061 tcgtcccctg gagtgttacc gcactcgcag gggaaccccc tcaaaatcgt tcatttttta
     3121 accagaagtt tttatctctt ttccacccat ttttaccgct caaagccgcg ccaccactgg
     3181 gctaagccaa aataacacat ccttgagttg ttgcctatag tataaataca aggatcgcgc
     3241 gcgcgatcgc ccacccaaaa tgcgcgtttt ttgagcgttc ccgagaacaa aaaaacaaca
     3301 catccaaacc acctgatcag caggtttctc accgactttc tgaacaacaa aac
//
LOCUS       DAZGBC010000297         3343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGBC010000297 DAZGBC010000000
VERSION     DAZGBC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.299"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1166..1360)
                     /locus_tag="ACHK1J_005002"
     CDS             complement(1166..1360)
                     /locus_tag="ACHK1J_005002"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7914135.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYTGAWKKWHDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(1934..2950)
                     /locus_tag="ACHK1J_005003"
     CDS             complement(1934..2950)
                     /locus_tag="ACHK1J_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO7914136.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLVERPAARWLFMTLTVKNPPVENTRETLIRMNAAWKRLSDRKEFRPVLGWLRTTE
                     ITYGRVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKQPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLVGADIPAEPDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT          871 a          891 c          703 g          878 t
ORIGIN      
        1 acagatttca atctggctgc gtgagacgtt ggcggggtgt cggggcgcag ccctgaccaa
       61 gtggtaatcg tatagccgtg cgtgcgcggt tatacgatta cacatcctgt cccggaatca
      121 ggattgtttt tcgcgcacaa ctgcggcaaa ttcaccccgt tgcggttgca acgatgggaa
      181 tatcaaaact acgaaaatta ttcaggcatt tgctacccct ctggccctgg cctccacctg
      241 ttccgggggg gatttttttg ttgcctctca tgacgaactg cggcaaattt ctgacgttgc
      301 aaactgcaac acataaaaca cacagaaaaa ctcacaattg ccatgtcctc attgacaccc
      361 ccttgccata tagggggatt ttttcgcctg tcctgattgt cttttgctga tagttacggc
      421 agattggaaa agagcgcatt tgtggtgctc tctccaaaat aatatttctc attactcaaa
      481 ctctgatggc ttccccctgt aatccagggg gattttttta atttagcgct atgcttcgta
      541 attcattcag aatacagaca gtattgttaa tcagtttttc atcatgaacg gtgtgttcag
      601 caataagagt attaaactca ataatcaggc tgtaactctc tctgtataca tcatctctgt
      661 taaattcgtt cgctctgtat tttgaataaa taataatgcc tttgtcctgt atactctgaa
      721 tttgtttaat tacatcaata ctcataaaaa catccaacaa gaaccgactg taggtcaccg
      781 ggcaaacgag cgaaatgcta gcattttgcg gcgtttgccc tatcctgcat cagaacgcta
      841 aatacaggaa tctctttatg acgattcctc catttcaaga ttttttatgt ggcgtctact
      901 acatgcgatc cgttggtgaa ccgttagtta gcctttagat ggaccttaga tggaccttag
      961 atggacacta gttagatatt tactgattta gatgaacctt aaatggatct tagatggata
     1021 ttagatggat cttagttaga cattatgtag ataggaaata ttatggggca atcctagagg
     1081 cacgtgaccg actgtataca aaatgctggc gcattttgat acgtcgacgt gccaaagggg
     1141 acgccccttt gaaaccccgc cagaattaaa accggcctgt ctttttacaa tattcagctc
     1201 tggctataca ccagtctata ttttcgcctt tggcataatg aagcgcatca tgccattttt
     1261 tccatgctcc agtgtaatcg ccttcacgct ccagtctgac agcttcgttt gcgggtgaaa
     1321 aatatttatt ttctaaataa attgctttgt cctgattcat actcgtaatt tatattttta
     1381 tttgaattaa aaaagagcgg cctgtcgggt taatacatac tggcatgact gttgatatat
     1441 cagcccaact gcacgctcgt aatcgtttta acgtgatttt cgcaacatgg caatccgggt
     1501 tcatctgtcc ggtaatcaat aaaaaccgtg cgaaaaacgg gacaggatgt gggaatgtcg
     1561 gaccgcgcac gcgcactcag acattcccca cctggtcagg gctgcgcccc gacaccctcg
     1621 acaccgttcc cgcaatacga tagaacatca gggcgcgttt ccgggtaaac gcatgttaat
     1681 gaccgggaat tcattgatta gggaccggag acgcgctaca aaactcctgc gcgtttctgg
     1741 catcaactga ccatggggat ggtttggcag tagcagcaag cgccagaaac gcgctcaaaa
     1801 cgtctctgac ggcctgaatc caacaaaaca tacaaaacca ttgcccgatg caaaaaagtg
     1861 cgttagaaag cgtctgagcg cgtttctggc gtcttttttg ttgctgtgtc gctactcgtt
     1921 gtcagattcg cgctcaatcc ttcatcctgt cagcttcagg attccgcttg taacgttttt
     1981 ccgtacgtcc ccagtcaaac gccagccaga aggcttgctc ctcgaactcg tccggttctg
     2041 ccggaatatc tgccccaacc aaatcctcgt ctgtctccgg tttatcgact ttcagaacct
     2101 ctttgagacg accgccggtt gccaccatcc gaagcccgta ggtctgccgc gccagctcca
     2161 gaaaccacgc cggatcacgc acaacttccg aagatttcac cgtgtatttc atcgtctcaa
     2221 tgacgcctga ctccagcgcc gcacgatgca tatcaggcag ggttacacca tccggctgtt
     2281 tccagccctg tttcggtttt accacccgaa tatccactcc cgcctcataa tcgacacgaa
     2341 ggcattcact ccagatctcc acccagcgag catgtttcac ataaccatta ccggacaaca
     2401 tcgaaggggg gaccatcatc agaacgtgaa aatgcggatg gcaacatcct ggcactcgcc
     2461 cgtaagtaat ttccgtcgta cgtaaccagc caagaaccgg cctgaattcc ttacgatcac
     2521 tcaagcgttt ccatgctgca ttcatccgaa tgagtgtttc gcgggtattt tccacagggg
     2581 gatttttgac agtcagcgtc atgaacaacc agcgggccgc aggacgttca accaacagct
     2641 ttggcaaggc ctcccagaga cgacgcatcc aacggagaga gcgacgacga ctacacaccg
     2701 gacaaaacgg aacatgacaa aactccccct tccgaagagc cagcttcatt tcgccagtca
     2761 ggcgggacac cttcggcgca aacaacaacc gacgggaaca atcaaacatc cgctctccac
     2821 gagaggaaaa catcgaatcg ctttcgtaaa tctgcgccac cctgtcagcc atcacgcgat
     2881 cgctgtccca ttttgtatcc cgtgtgctgt aatcgcgcaa cgagagtacc ggactatctg
     2941 gatttttcat ttgcaccccc caagaactgg gcagatgcgg aaattttgtg ttagcatttt
     3001 tctgtcacct gtgactctgt tgccgaacaa cgaattccaa actggtgatc gtcccctgga
     3061 gtgtgaccgc actcgcaggg gaaccccctc aaaactgttc attttttaaa caaacatttt
     3121 tatctctttt ccacccattt ttaccgctca aagccgcgcc accactggcc taagccaaaa
     3181 taacacagac cgcagttgtt gcctatagta tatatacaag gatcgcgcgc acgatcgccc
     3241 accaaaaatg cgcgtttttt gagcgcttcc gagcacaaaa aaaacaacac atccaaacca
     3301 cctgatcatc aggtttctca ccgactttct gaacaacaaa atc
//
LOCUS       DAZGBC010000298         3318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGBC010000298 DAZGBC010000000
VERSION     DAZGBC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.300"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..768)
                     /gene="sctW"
                     /locus_tag="ACHK1J_005004"
     CDS             complement(<1..768)
                     /gene="sctW"
                     /locus_tag="ACHK1J_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0050709 - negative regulation of protein
                     secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HFO7914137.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQS"
     gene            complement(773..2476)
                     /gene="sctC"
                     /locus_tag="ACHK1J_005005"
     CDS             complement(773..2476)
                     /gene="sctC"
                     /locus_tag="ACHK1J_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO7914138.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGGNVLSEISSRQQLKNVSRMPPFPRAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(2473..3222)
                     /locus_tag="ACHK1J_005006"
     CDS             complement(2473..3222)
                     /locus_tag="ACHK1J_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7914139.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          946 a          725 c          545 g         1102 t
ORIGIN      
        1 cgactgaaga gacaatagtt gtgacaactt attgagaaac ataccaaatt ccaatgatga
       61 acaactcgca tcaagtgcat ttatatctga gaaaagcgtc tctttgataa attttgtcac
      121 caacattcta ttttgatagc cataactacc aatccattcg acataagtat ctacagcatc
      181 gtcttctgcc attaaaaatt gccgataact cgcccttagt aattttggat ttaatgacag
      241 cgcctggcca aataactttg cttttaatgc cgagttaatg ccgctattaa tgaatttttt
      301 agtagtctct tgattaactt ttctaagtaa tgcttcagcg tttagccgga cattttctga
      361 tatttgtttt ttacgtaaaa ttgcacgtaa cacaactacc agatcgcttt catctgggaa
      421 taatttttta gcaaagctat acacttcata gtcatgggta gttgcagcct taagcgcctc
      481 cataactttg tcggtttttt ctattggctg gtcttccagg acatattctt ctccgtccag
      541 aaaattggcc agttgttctt cgtagatttt tttcttcata aactgcgtta acgctgctga
      601 catctcgtca gtagcttgta agaaccgagc ttctttacct gcgggatcat cccccatttt
      661 aggcatttca gcttcaatat cttcattatc ttctgtgata atgtcctcta agcgagaaac
      721 ttccctggct ctttctaata caccgacatg ctctacatga atcgccattt cactatcctc
      781 cgacgacttc acgattcagg taagttttta tccatttctg cagtaattca tcgttaattt
      841 ctttattttt tgccatttgc tgtgtaatcg ctctggcatc aaccgcagcc tcattcgcat
      901 tattcatcat gcgctcatcg atctctcttg gctcaataag aaagacacga acgatgttat
      961 tcgcatttgt tccttcataa ccaaataatt taccaataaa tggaatgctg cctaatattg
     1021 ggatcttgcg actttcatag gtattcgtat cgcgtgtata tcccccaatc aaaagacttt
     1081 tcccttgggg cacgcgtgca atagtactta taagtgttct accaacctga ggcagatcat
     1141 caacattggt cttgtcggaa ttgatttcat ttccatcttc aatattcaag agtaattcaa
     1201 tttgatttcg cgcagcaaat cgtggtagca cgctaatcat cgtaccatag gttacttcat
     1261 ccaattccgc agtacgctcg ccgactaatt tagtatagaa agtgcggtta ttatcaaata
     1321 tagcaggaat attctcctgt gttaaaacga cgggacgagc aacaacggcg gctcttcttt
     1381 tttgtgcaag tgcctgtatt gttgcaataa actgtgtccc atcaagggta ctaattgaac
     1441 ctgaattatt aaatgatgca cttaagctgg atccaatttt tatcgtgccc gaccagtcag
     1501 cacctagctg ttctagatca gtcttatcaa tatctattat ccataaagat aattcaatgt
     1561 gtcgctttgg aatatcaagc gtagctacta acttctcgat aaaatcaacc tgagatactg
     1621 ttcctttaac aagtaaactg ttggtatcag gatatgcaat aatttgtata tcgtcagtct
     1681 ctggcgctcc tgctgtcgat attattttct ctacttgtag gtcatcatct tcttcagccc
     1741 taggaaaagg aggcatacgt gatacgtttt ttagttgttg acgacttgaa atttcagaaa
     1801 gtacgttccc ccctgtgctt tgcttaatgt tattatttaa caacgtcgat agtacttttg
     1861 ccatgccagg aataactatc ttttcgccac gtaactcata agtccggtca ttaacgaatg
     1921 tgttgaccag atgaattatt cctactttat ttcgaccgat ctcaatgcca tcgctgtttt
     1981 gctccattaa tttcgcagca ttaacgacca ggtcaacata aaccggtggc cccgatacat
     2041 agaaagtacc attgccgccc cctttaattt cataacgaga gttatacaga cccgattttt
     2101 ttaagaaatt attaaactca ttcgtcgata cctttctcag attaatcaat gcattacgca
     2161 tctctgatga gtcatagata taaatagctt tgccatcatc ataccaaata agccccatct
     2221 gtgccgacag gcgttcaatc aaggcatagg gttctgttaa atcaaaattt ccgctgatct
     2281 gtttcttcat ggcaagctta ctaactattg taggcttacc cgcatatgat gaaaccgttt
     2341 caaaaaaact acgtagattt tctttatttg ctacgtatcc attcgctgct gcggcatatg
     2401 caccaggagt caataatcca ttaaaaatgc ataagactga aattaatata atagtaatgc
     2461 gtaattttat tttcataact ttttacatag ttctctcggt gtttttccta accggctttt
     2521 tacttcggct gaaaagtgtg atgaggacga gtaaccatat ttatgcgcaa tagttgtcat
     2581 atcactgttt ccttcaatga tttccaacac ggctctggca actctccagc cacataattc
     2641 cgttttaact ttaccaccca atgcataact acataaacgg cgaaaatgtg aatacgatac
     2701 gccgtaaagc tcaccaagat catagagtga agtctgtatg agcgattgcg ataataaata
     2761 acgaaccagt ttatagcatt ctgtttttct tagatgctga aacaggattt cattaatatt
     2821 atcgctgctc agataagtgg ctaaaaacca ggcttcactt gcaactttac tgaatagcac
     2881 aacttctgat gcaaagggca aggcatcagc aaacaattca tgatgttccc tttccacact
     2941 ccccgttgat tcatctataa aagctaataa tttactcagg atatcaatgc gaagtggatg
     3001 ataaattact ttccctgcag ttacctcaat ttggcttcgg tcaatgagca gtaatatatc
     3061 ttccactacc attaattttt gatggttatt accaatcgta ataacaaggg ttacatcctt
     3121 gttttcattt ccccgtaaga accacatttc agggtccagt agggttaact tctgtccttg
     3181 aagcaaaaca ttcattggaa gtaacagccc ttcttcaatc atgtttcatc tccttattcc
     3241 atattctcat tctccataaa cttgctataa aaacatgatt aaaaagtgat gattactatt
     3301 tataaaataa tgattttg
//
LOCUS       DAZGBC010000299         3257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGBC010000299 DAZGBC010000000
VERSION     DAZGBC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.301"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..649)
                     /locus_tag="ACHK1J_005007"
     CDS             complement(<1..649)
                     /locus_tag="ACHK1J_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFO7914140.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKRFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHR"
     gene            842..1345
                     /locus_tag="ACHK1J_005008"
     CDS             842..1345
                     /locus_tag="ACHK1J_005008"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7914141.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPVIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1391..1807)
                     /gene="rraB"
                     /locus_tag="ACHK1J_005009"
     CDS             complement(1391..1807)
                     /gene="rraB"
                     /locus_tag="ACHK1J_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO7914142.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1969..2973
                     /gene="argF"
                     /locus_tag="ACHK1J_005010"
     CDS             1969..2973
                     /gene="argF"
                     /locus_tag="ACHK1J_005010"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO7914143.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSN
                     "
     gene            complement(3030..>3257)
                     /locus_tag="ACHK1J_005011"
     CDS             complement(3030..>3257)
                     /locus_tag="ACHK1J_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7914144.1"
                     /translation="TNEIVKKYPGYVVKIFEEKMGNELKIRTRMMETLRDGKINIYDY
                     INEKQLEKLNPPQDLRSAIEKIGWKNRPITA"
BASE COUNT          944 a          744 c          701 g          868 t
ORIGIN      
        1 aacgatgtat accgaaactg gtattgctcc agagaaaact cattaccagg ctatataaag
       61 taccattaaa aataccaatg gaaacaatac caactattgc catcagaacc acagatataa
      121 taacactact tgaactattg gcatgtagca tctttgtcga gataaagaaa acagtcccca
      181 tcccaatggc cattaaaatc ggcaagaaaa aggttaccca ccagaacctt ttcatagagc
      241 aattaaaact aaatcttacg ccgttgagac tggtcatcaa ggcctgataa cgcagtcctt
      301 ttgctgccat aaatataagc aaaaccaaca gtgacaaagt caaaacacag ccaacaagcg
      361 gcatatctgc tgacactgtc attaagattg cgaaatagaa aaaaacaaaa acaagacaac
      421 taaaaaaaac attcccacca gtaattccat aagaaaatcg ttgtccgtta acttccatat
      481 tagcatagag ataacgctta catttcatta atgcccatgg taaatagata cctagcgtta
      541 taatcgttaa caacacattc accacacaaa taagaaaata tctctcacct gtaccatgaa
      601 aaacaaacga atgggacgga acatccattt cattaataac ttgagccata aaaattccct
      661 gaattaaaaa atccattgat ataatttata gatacagaca gtaaaatgtt ctgccacgtt
      721 acaatactct tatcagtaat aggctggcaa aaactttatt gctttataag atgcttgcgc
      781 acgtaagaag agagtggtat taaacaaatg tataattact ttatttttat tgggttaaac
      841 tatgaataac attgcgccgc aatcacctgt aatgcgtcgc ctgacgctgc aggataatcc
      901 tgttattgcc cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg ctgataaagg
      961 ctacaccgtc gccgatccga atcttgacga actgtatcaa gtgtatagtc agccgggtca
     1021 tgcatattgg gtggttgaat acgagggtga agtggtcggc ggcggtggga ttgcgccatt
     1081 agcaggtagt gaatcggaca tttgcgaact gcaaaagatg tattttcttc cggctattcg
     1141 cggtaaaggg ctggcgaaaa aactggcctt aatggcgatg gagcaggcac gagaaatggg
     1201 cttcaaacgc tgctatctgg aaacgaccgc ttttttaaag gaagccattg cgctttatga
     1261 gcatttgggc tttgagcata tcgactatgc gcttggctgc acgggccatg tcgattgtga
     1321 agtgcggatg ctacgcgaac tctaaaaaaa tgcccgctag tgtgtgctat cgggcatttt
     1381 aatcaattaa ttagtggcga accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc
     1441 catcttcgcc gttaggatct tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt
     1501 tctccgccag cgtcatcagt tgttcaacct gggcatcgat cagatcggca ttcaacgcgc
     1561 actcgctgag gatgtcgcag caaatcacga tatcaccatc ttcaacttcc agctcttctg
     1621 gatcggtcac ttcgtaaccg agtttaaacg cttcaactgc tgctttttcc agggtttcca
     1681 gatcgtctgc ggaaagatgg tgttcgatgg tgtacagcgc gtccggatcg ctgccatctt
     1741 ccagtaattc ttcaataatc aaacgtgttt cttcacgctg ttcttccagt tgttccgggt
     1801 ttgccatggc tcattcctct taatgtcctg ccgatactca tattgtcaca caccactggc
     1861 attgcctcca cctttgtgac aaagatttat gctttagact tgcaaatgaa taatcatcca
     1921 tataaattga attttaattc attgaggcgt tagccacagg agggatctat gtccgggttt
     1981 tatcataagc atttcctgaa attactcgat ttcacgccag ctgaactcaa cagcctgctg
     2041 cagttagccg cgaagctgaa agccgataag aaaagcggca aagaagaagc caaactcact
     2101 ggtaaaaaca tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg ctctttcgaa
     2161 gttgccgcat atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg cagtcagatt
     2221 ggtcataaag agtcgattaa agacactgcc cgcgtgctgg gtcgcatgta tgacggtatt
     2281 cagtatcgcg gctatggtca ggagattgtc gaaacactgg cggaatacgc tggcgtgccg
     2341 gtatggaacg gcctgaccaa tgagtttcac cccacgcagt tgctggcgga tctcctcacc
     2401 atgcaggagc atttacccgg taaagcattc aacgaaatga cgctggtcta tgcaggtgac
     2461 gcacgtaaca acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg
     2521 cgtttggtcg cgccacaggc atgctggccg gaagctgcgc tggttacgga atgccgcgct
     2581 ctggcacagc aaaatggcgg gaatattacg ctgacggaag atgtagctaa aggcgttgaa
     2641 ggtgctgact ttatctatac cgatgtatgg gtgtcgatgg gtgaagcaaa agagaaatgg
     2701 gcagaacgga ttgcattact gcgtgattat caggtgaaca gcaagatgat gcagttgacc
     2761 ggtaacccgg aggtcaaatt cctccactgc ctgcccgcgt ttcatgacga ccagacgacg
     2821 cttggtaaga aaatggcgga agagtttggc ctacatggcg gaatggaagt gactgatgag
     2881 gtcttcgaat ctgccgccag cattgttttt gatcaggcgg aaaaccgtat gcataccatc
     2941 aaagcagtga tggtcgcgac gctcagtaac taattgatat cgctataaag atgccgggaa
     3001 atacaaaact atcccggcat cacattattt tatgcagtga taggtctatt cttccaccca
     3061 attttttcta ttgcagaacg taaatcttgc ggtggattta atttttctaa ctgtttttca
     3121 ttaatataat catatatatt gattttccca tcccgcaggg tttccatcat tcttgttcta
     3181 atttttaatt cattacccat cttttcttca aaaatcttaa cgacataacc cggatatttt
     3241 tttacaattt catttgt
//
LOCUS       DAZGBC010000300         3177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGBC010000300 DAZGBC010000000
VERSION     DAZGBC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.302"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..614)
                     /gene="mobB"
                     /locus_tag="ACHK1J_005012"
     CDS             complement(87..614)
                     /gene="mobB"
                     /locus_tag="ACHK1J_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO7914145.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
     gene            complement(596..1180)
                     /gene="mobA"
                     /locus_tag="ACHK1J_005013"
     CDS             complement(596..1180)
                     /gene="mobA"
                     /locus_tag="ACHK1J_005013"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO7914146.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1250..1519
                     /locus_tag="ACHK1J_005014"
     CDS             1250..1519
                     /locus_tag="ACHK1J_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO7914147.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1596..2582
                     /gene="srkA"
                     /locus_tag="ACHK1J_005015"
     CDS             1596..2582
                     /gene="srkA"
                     /locus_tag="ACHK1J_005015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO7914148.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFNHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2599..>3177
                     /gene="dsbA"
                     /locus_tag="ACHK1J_005016"
     CDS             2599..>3177
                     /gene="dsbA"
                     /locus_tag="ACHK1J_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO7914149.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVF"
BASE COUNT          798 a          775 c          780 g          824 t
ORIGIN      
        1 aggccatcct gacggatggc ctttttgcgt tggtgcaaaa aaatgccgga tgcgacgctg
       61 gcgcgtctgc tccagcctac tcttgcttat ccgtcctgat tttgcatcca ctccaccaca
      121 aaatcagcca gcccctcaac atcattaata tccagtaatg cgacatcaag attaagcggc
      181 acatcactgg ctacagcaat aacatgcctg tctatcacta attcttcagg tcgatgtccg
      241 gctccatcgc gaaacagcac aatctttgcg atctcttcat gcttaaaccc ttcgaccaga
      301 atcaaatcca gctttgaggt atccattcga cttgcgagaa actgtagatc cagctcttct
      361 tcgtctggtg tttccgtcat caaggcccat cgttgctggc tggcaacgat ggtttgtgcc
      421 gcgccagcct tgcgcagctc atagctatct ttgcctggct tatcaacatc catatcatgg
      481 tgcgtatgct taatcagccc tggacggatc cctctggcgc ataatgccgg gatcagtttt
      541 ttcaaaagcg tagtttttcc ggtaccactc cacgcggcaa aggcgagtaa cggtatcatc
      601 gtttttcctg ccatcgggca agctcctctg gcgtattcac gttaacaaat gcatctttat
      661 gatcgctgaa atcaaccgca tgaccgccag ccagacgcat aaatgccatt acccggcgtt
      721 ctcctgcttg cagatattct agtaataaag gctcaatagc gcggtttacc agagcaatag
      781 tcgggtgatc gcgttcaccg tcatggaccc acacgacagg cgcatctttg cgctgatgat
      841 taagccgggc ggctaaatca tggggaatgt aaggcgtatc gcacggacaa aacaaaaacc
      901 actcaccagc ttcctgctgc attactgaaa gcatccctgc cagagggcct gggtaatccg
      961 ccagtgaatc ttcaatcact ttcagaccgc ctgcctgata gatttcctga tgacgattag
     1021 cattaaccac gacgtgagag agctgcgtca taagcgcgtc agcgacatgt tgccataatg
     1081 gtttgccgtt taattcaagc aatcctttat ctacgccgcc cattcgtctg gctttaccgc
     1141 ctgccagcac aacgcctgtt atcgtcgtca tcagattcac cgatatcgcc tcttttattg
     1201 tgggattgac cctgctaacg tgtctgtctc aagagtaagg agcatcacta tgaaatgtaa
     1261 acgtctgaat gaagttattg aactcctcca gccagcctgg caaaaagagc cagaccttaa
     1321 cctgctgcaa tttttgcaga aactggcgaa agagtcaggt tttgacggcg aactggcgga
     1381 tttgacggat gacattctga tctatcacct gaaaatgcgt gattccgcga aagatgcggt
     1441 gatcccgggt ttgcagaaag attatgaaga agacttcaaa acggcgctgt tacgcgctcg
     1501 cggcgtaatt aaagagtaaa agcttgtaag cggcgccacc aaaatcatcg tgaaatgata
     1561 tccttcatca ttcgtaatgt tttccggatg atgggatgaa caacagcgct tttactttcc
     1621 agacactaca cccggatacc atcatggatg ctctgtttga gcaagggatc cgggtggatt
     1681 ccggtcttac cccgcttaac agctatgaaa accgtgtcta tcaatttcag gacgaagatc
     1741 gtcgacgttt tgtcgtcaaa ttttatcgcc ctgaacgttg gacagccgat caaatcctcg
     1801 aagaacatca atttgcgttg cagctggtaa atgatgaagt cccggtcgca gcacctgtgg
     1861 cctttaacgg tcagacttta ttgaatcatc agggatttta tttcgctgtt tttccaagcg
     1921 tcggtggtcg ccagttcgaa gctgataata tcgatcagat ggaagcggtt gggcgttatt
     1981 tagggcgtat gcaccagacg gggcgcaaac agctttttaa ccatcgcccg accatcggtc
     2041 tgaatgaata tctcattgag ccacgcaagc tgtttgagga cgctacactg ataccttccg
     2101 ggttgaaagc ggcattcctg aaagcgacag atgagctgat cgccgccgtt acagcacact
     2161 ggcgggaaga tttcaccgtt ctgcggctac atggagactg ccacgccggg aatattctct
     2221 ggcgcgatgg cccaatgttt gttgatctgg atgatgcacg taatggtcca gctattcagg
     2281 atttgtggat gttgctcaat ggcgataaag ccgagcagcg gatgcaactg gaaactatta
     2341 ttgaagctta tgaagaattt agcgagttcg acaccgctga aatcggactg attgaacctt
     2401 tacgcgccat gcgtttggtt tattatcttg cctggctaat gcggcgttgg gctgatcccg
     2461 cgttcccgaa aaatttcccg tggttaaccg gggaagatta ctggctacga cagacggcga
     2521 cttttataga acaggcaaaa gttctacaag aacccccttt gcaattaaca cctatgtatt
     2581 aatcggagag agtagatcat gaaaaagatt tggctggcgc tggctggttt agttttagcg
     2641 tttagcgcat cggcggcgca gtatgaagat ggtaaacagt acactaccct ggaaaaaccg
     2701 gtagctggcg cgccgcaagt gctggagttt ttctctttct tctgcccgca ctgctatcag
     2761 tttgaagaag tactgcatat ttctgataat gtgaagaaaa aactgccgga aggcgtgaag
     2821 atgactaaat accacgtcaa cttcatgggt ggtgacctgg gcaaagatct gactcaggca
     2881 tgggctgtgg cgatggcgct gggcgtggaa gacaaagtga ctgttccgct gtttgaaggc
     2941 gtacagaaaa cccagaccat tcgttctgct tctgatatcc gcgatgtatt tatcaacgca
     3001 ggtattaaag gtgaagagta cgacgcggcg tggaacagct tcgtggtgaa atctctggtc
     3061 gctcagcagg aaaaagctgc agctgacgtg caattgcgtg gtgttccggc gatgtttgtt
     3121 aacggtaaat atcagctgaa tccgcagggt atggatacca gcaatatgga tgttttt
//
LOCUS       DAZGBC010000301         3169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGBC010000301 DAZGBC010000000
VERSION     DAZGBC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.303"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>3169)
                     /locus_tag="ACHK1J_005017"
     CDS             complement(<1..>3169)
                     /locus_tag="ACHK1J_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HFO7914150.1"
                     /translation="MADNGAAVASTMTTKPDGTVEISVTSQTAGTSTVTASINNSSQS
                     RDVTFIADVRTAQIADLVVIKDSSEADGATANTLRARVTDAFGNALAGQTVSVLADNG
                     ATVSPTVTTEPDGTVEISVTSQTAGTSVVTVSINNSSLSRNVTFIADVRTAKIADLAV
                     TQDGSVADGATANTLRVRVTDAFGNTLAGQTVSVLADNSATVTPTVITGQDGTVEISV
                     TSQTAGTSVVTASINNSSLSRDVTFIADIRTAQIADLVVIKDGSEADGATANTLRARV
                     TDAFGNALAGQTVSVLADNGATVSPAVTTEPDGTVEISVTSQTAGISTVTATINSSSL
                     SRNVTFIADVRTAQIADLVVIKDGSVADGSTANTLRVRVTDAFGNALGGQTVSVLADN
                     GATVASTVTTGQDGTVEISVTSQTAGVSAVTATINNSSLSQSVMFIADVRTAQIAELV
                     VIKDGSEADGATANTLQVTVTDAFGNTLAGQTVSVLAGNGATVAPTVITEPDGTVEIS
                     VTSQTAGISTVTASINSSSLSRNVTFIADVRTAKIADLVVIKDDSVADGAMANMLQVK
                     VTDAFGNALNGQTVSVSADNSAMVTPTVTTQPDGTVEISVTSQTAGISTVTATINNSI
                     LSRNVMFVADVRTAQIADLVVIKDDSVADGAMANILRARVTDAFGNTLAGQTVSVMAD
                     NGATVALTMTTKPDGTVEISVTSQTAGISTVTATINNSTLSQNVTFIADIRTAQIADL
                     VVIKDDSVADGAMANMLRARVTDAFGNALAGQTVSVFAGNGATTAPTVTTQPDGTVEI
                     SVTSQTAGTSAVTASINSSSQNRDVTFIADVRTAKIASLEVTQDNAVADGAMADMLRA
                     RVTDAFGNALAGQTVSVMADNGAAVASTMTTKPDGTVEISVTSQTAGVSAVTASINNS
                     SLSRDVTFIADVRTAQIADLVVIKDGVVADGAMANMLRVRVTDANGNTLGGQTVSVTA
                     DNSAMVASTVITGPDGTVELSVTSQTAGTSTVTASINNSSLSRNVTFIADIRTAQIAD
                     LVVIKDDSVADGAMANMLRARVTDA"
BASE COUNT          515 a         1095 c          834 g          725 t
ORIGIN      
        1 aacgcatcgg tgaccctcgc ccgcagcata ttcgccattg caccgtctgc cactgagtcg
       61 tccttaatga ccaccaggtc cgcgatctgt gccgtcctga tatcggcgat aaatgtcacg
      121 ttccggctca ggctgctgtt gttgatgctg gcggtgactg tactggttcc ggcggtctgg
      181 ctggtgactg agagctccac cgtgccgtcc ggccctgtga tgacagtcga agcgaccatt
      241 gcactgttgt ctgccgtcac gctgaccgtc tgtccgccaa gcgtattacc gttagcatcg
      301 gtgaccctca cccgcagcat gttcgccatt gcgccgtctg ccactacgcc gtccttaatg
      361 accaccagat cagcgatctg cgccgtcctg acatcggcga taaatgtcac gtcccggctc
      421 aggctgctgt tgttgatgct ggcggtgacc gcactgactc ccgccgtctg gctggtgact
      481 gagatctcca ccgtgccgtc cggctttgtg gtcatcgtcg aagcgaccgc tgcaccgttg
      541 tccgccatca cgctgaccgt ctgcccggca agcgcattac cgaacgcatc ggtgaccctc
      601 gcccgcagca tatccgccat tgcgccgtca gccacggcgt tatcctgcgt cacctccaga
      661 cttgcgatct tcgccgttct gacatcggcg ataaacgtca catcccggtt ctggctgctg
      721 ctgttaatgc tggcagtgac cgcactggtg cctgccgtct ggctggtgac agagatctcc
      781 accgtgccgt ccggctgcgt ggtgaccgtc ggagcagtcg tggcaccgtt gcctgcgaac
      841 acgctgaccg tctgcccggc aagtgcatta ccgaacgcat cggtgaccct cgcccgcagc
      901 atattcgcca ttgcgccgtc agccactgag tcgtccttaa tgaccaccag gtccgcgatc
      961 tgcgccgtcc tgatatcggc gataaatgtc acgttctgac tcagggtgct gttgttgatg
     1021 gttgcagtga ctgtactgat ccccgccgtc tggctggtga cactgatctc caccgtgccg
     1081 tccggctttg tggtcatcgt caaagcgacc gttgcaccgt tgtccgccat cacgctgacc
     1141 gtctgcccgg caagcgtatt accgaacgca tcggtcaccc tcgcccgcag aatattcgcc
     1201 attgcaccgt ctgccactga gtcgtcctta atgacaacca gatcagcgat ctgcgccgtc
     1261 ctgacatccg cgacaaacat cacgttccgg ctcaggatgc tgttgttgat ggttgcagtg
     1321 actgtactga tccccgccgt ctgactggtg acactgatct ccaccgtgcc atccggctgc
     1381 gtggtcaccg tcggagtgac cattgcgctg ttgtctgccg atacgctgac cgtctgccca
     1441 ttcagcgcat tcccgaatgc atcggtgacc ttcacttgca gcatattcgc cattgcaccg
     1501 tctgccactg agtcgtcctt aatgactacc agatcagcga tcttcgccgt cctgacatcc
     1561 gcaataaacg tcacgttccg gctcaggctg ctgctgttga tgctggcagt gactgtactg
     1621 atccccgccg tctggctggt gacactgatt tccaccgtcc cgtccggctc tgtaatcacc
     1681 gtcggggcaa ccgttgcgcc gttgcctgcc aacacgctga ccgtctgccc ggcaagcgta
     1741 ttaccgaacg catcggtgac cgtcacctgc agcgtattcg ccgtcgcacc gtctgcctct
     1801 gaaccatcct taataaccac cagctccgcg atctgcgccg tcctgacatc ggcaataaac
     1861 atcacgctct ggctcaggct gctgttgttg atggttgccg tgactgcact gactcccgcc
     1921 gtctggctgg tgactgagat ctccaccgtg ccgtcctgcc ctgtggtgac cgtcgaagcg
     1981 accgttgcgc cgttgtccgc caacacgctc accgtctgcc cgcccagcgc attaccgaac
     2041 gcatccgtca ccctcacccg cagcgtgttc gccgtcgaac cgtctgccac tgagccatcc
     2101 ttaataacca ccagatcagc gatctgcgcc gtcctgacat cggcgataaa cgtcacgttc
     2161 cggctcaggc tgctgctgtt gatggttgca gtgacagtac tgatccccgc cgtctggctg
     2221 gtgacactga tttccaccgt gccgtctggt tctgtggtga ccgccggaga caccgttgcg
     2281 ccgttgtccg ccaacacgct gaccgtctgc ccggcaagcg cattgccgaa cgcatcggtc
     2341 accctcgccc gcagcgtgtt cgccgtcgca ccgtccgcct ctgaaccatc cttaatgacc
     2401 accagatccg cgatctgcgc cgtcctgata tcggcgataa acgtcacatc ccggctcagg
     2461 ctgctgttgt tgatgctggc agtgaccaca ctggtgcccg ccgtctggct ggtgacactg
     2521 atttccaccg tgccgtcctg ccctgtgatg acagtcggag tgaccgttgc gctgttatcc
     2581 gccaacacgc tgaccgtctg cccggcaagc gtgttcccga acgcatccgt caccctcact
     2641 cgcagcgtgt tcgccgtcgc gccatctgcc actgagccgt cctgcgtcac cgccagatca
     2701 gcgatcttcg ccgtcctgac atcggcaata aatgtcacgt tccggctcag gctgctgttg
     2761 ttgatgctga cagtgaccac actggtgccc gccgtctggc tggtgacact gatctccacc
     2821 gtcccgtccg gctctgtggt aaccgtcgga gacaccgttg cgccgttgtc cgccaacacg
     2881 ctgaccgtct gcccggcaag cgcattgccg aacgcatcgg tcaccctcgc ccgcagcgta
     2941 ttcgccgtcg caccgtccgc ctctgaacta tccttaatga ccaccagatc cgcgatctgc
     3001 gccgtcctga catccgcgat aaatgtcaca tcccggctct gactgctgtt gttgatgctg
     3061 gcagtgactg tactggttcc ggcggtctgg ctggtgactg agatctccac cgtgccgtcc
     3121 ggctttgtgg tcatcgtcga agcgaccgct gcaccgttgt ccgccatca
//
LOCUS       DAZGBC010000302         3150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGBC010000302 DAZGBC010000000
VERSION     DAZGBC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.304"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..325)
                     /locus_tag="ACHK1J_005018"
     CDS             complement(<1..325)
                     /locus_tag="ACHK1J_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914151.1"
                     /translation="MFRLAKPEDYEFIPDINLWELSFDKRPVRGVRCEDPVLGAQQYN
                     DTQQSILLNRQRQKLRKQNFFSLENISWDGIFDWCLSKGTQEECYLIVKLYYAKNKDE
                     HYSTLS"
     gene            406..621
                     /locus_tag="ACHK1J_005019"
     CDS             406..621
                     /locus_tag="ACHK1J_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914152.1"
                     /translation="MSDKIQEITQKIEKLQIKLSKIEEEKNKIQSKLVKLEEEKKSIQ
                     RKLILQEKREELEMLLDKESFDILFNK"
     gene            1942..2634
                     /locus_tag="ACHK1J_005020"
     CDS             1942..2634
                     /locus_tag="ACHK1J_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914153.1"
                     /translation="MIYRFKTDFMRQIAMLFSLGYTRWFGGEIPLNPHYKFVSLKARF
                     DELYAINATAQQRFRRREKGVANCKFLVWFDEKNATLLWVLLATEGDVYNEEKFYDGM
                     NKKTRISVSGYELKTVPHRGRKPSVSWAMKKETYEKWHADIKKSIRTRNDENIEKLWY
                     SLRRVAGFSELRRQVFKLQRYAMTEWKRSRRGEYPYEKIFVGWLGRFKQSETITDEEL
                     LLISKSKKADIF"
     gene            2661..>3150
                     /locus_tag="ACHK1J_005021"
     CDS             2661..>3150
                     /locus_tag="ACHK1J_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914154.1"
                     /translation="MQANDEFLRLVFENIWPWWTYAWVLVFSLLCVFTFSGHAEKSGD
                     KSTLRFIIEAARKKLLVAWILCTLLFFLFVYIIAWKHFGEDSHKASLYMELLYERIGD
                     KSFSFGDYCGIAFVLLFPYACQIINRRLIRPKLSSWLRRFRVSQTSDTMSDIRVEKGK
                     YSP"
BASE COUNT          915 a          611 c          662 g          962 t
ORIGIN      
        1 tacttaaagt gctgtaatgt tcatccttat tttttgcata ataaagtttg acaataaggt
       61 aacactcttc ctgtgttccc tttgataaac accaatcgaa tattccatcc catgagatat
      121 tttctaagct aaaaaagttc tgttttctta atttctggcg ttgtctgttt aaaagaatgc
      181 tttgttgagt atcgttgtat tgctgggcac caagaacagg atcttcacag cgaacgccac
      241 gaacagggcg tttgtcaaat gacagttccc ataggttaat atctggtatg aactcgtaat
      301 cttccggttt cgccagacgg aacataacgc tatcctttgt ctgttgtgtg ttcggtagtt
      361 ctggtttacc gtaaaacaaa aattttttca acaaaggtgt ttaatatgag cgataagatc
      421 caagaaatca cacaaaaaat tgagaaattg cagataaaat tgagcaaaat tgaagaggaa
      481 aaaaacaaga tacaatcaaa gctggttaag ttagaagaag agaaaaaatc aatacagcgc
      541 aagctgattc tacaggagaa aagagaagag cttgaaatgc ttttagacaa agaaagtttt
      601 gatattttat tcaataaatg agcatgattt ttgggagaat tgccctctga ttatcagagg
      661 gcaatttgtc attggtagtg cctattaccg ttagttatga tcgcgaaaaa cgcgattatt
      721 gttcattaca atgccctatc gttagacagg acatgtgagt gttttatcaa caacgtaagc
      781 agaatttttt gcagaaatga tcctgcatca atactgaaaa tcgttgttgt cttacatgtg
      841 ttaaacatga ttttttagtc aatttcataa caaaatatac ctacgttttt ttgggaagtc
      901 ctcttactga accaacagaa agaggacgaa aaagaggaaa catgaatgtt tcctctgcca
      961 tgttactacc gtgaattttc agctacttat ctgtactggt tgcctccagt attttcagat
     1021 tcatctacag acttcggcta tatcgtctgt agatttttgc gcctactcgt ccactctccc
     1081 acactcagga atgtaatttc ggtgccaccc gaaattttcc agagtgcttc ccacgttagc
     1141 tgatcttgtc gggataaccc gccactggct cagacaaact actaggctac gccttaccat
     1201 ttctggcttt cagggggccg agattccaaa gcacctccct tactcataat ttgccgtcaa
     1261 caccatagcc agacacttaa gtcatggctg gttatcactt acccgaatgc gacctcttcg
     1321 gacgcaatta caggcttgag ctttacagcg atctgcaaag gttgcccctc acagtaccga
     1381 cgtttccagg tttcccctca acgccctgac acctcacgat gccctcagca gcccacctgt
     1441 cgggaatatg ttgtaccttc tcaagcaccc catattcggg attttcaccc gttcaagatc
     1501 aacagggatc acgtagtcac ctacgttccc gtttccgtgt cgcccagtct cacgctttgg
     1561 cggggattgg gtcggacagg ggagtttcac ccccgaacgc ccttcgtagc cgtaccagcc
     1621 caattacagg catacggctt cggcagtaac tgttatcagg gttggcagtc ctgataactc
     1681 accttcgttt ggcagacgaa ggcttttggc ttttttcgtc tttcgacttc ccaaaaatgc
     1741 gtcgagagtt ggcagctcgc aacgcacgtt ttgttacgta agcgatgctt gatagtccgg
     1801 cgcccgaaag gctcgcatcg agccggacat cagtataata tttaacctgt aagcgcagcg
     1861 caaggaatct ctttataagg tattcccata tgttgacttc tcttatgtag cggttactac
     1921 atagctttta tgaggatttc gatgatttac cgttttaaaa ccgattttat gcgtcagata
     1981 gccatgctgt tttcgctggg atatacaagg tggtttggtg gcgaaattcc tctaaaccct
     2041 cactataaat tcgtgtcact aaaagcacgt tttgatgaac tctatgctat caatgcaaca
     2101 gcacaacaac gtttcaggag aagagaaaaa ggagtagcaa actgcaaatt tcttgtctgg
     2161 tttgatgaaa aaaatgcaac tcttttatgg gtgttacttg caacagaagg ggatgtatac
     2221 aatgaagaaa aattttatga tggtatgaat aaaaaaactc gtatttctgt ttcaggttat
     2281 gaactaaaaa cagttccaca tcgaggtaga aaacctagcg tttcatgggc tatgaagaaa
     2341 gaaacttatg aaaagtggca tgcggatata aagaagtcga taagaacaag aaatgatgag
     2401 aatatagaaa aattatggta ttcactcagg agggtggcag ggtttagtga gttacgtaga
     2461 caggtattta agttacagag atatgcaatg acagagtgga aacgttccag aagaggtgaa
     2521 tatccttatg agaagatttt tgttggatgg ttaggacgat ttaaacaaag tgaaaccatc
     2581 acagatgaag agcttttgtt aatatcgaag tcaaaaaaag ctgatatctt ttagttttat
     2641 caacatagcg tgaggtgctt atgcaagcaa atgatgagtt tcttcgcttg gtttttgaaa
     2701 acatatggcc ctggtggaca tatgcttggg ttttagtttt ttctttgtta tgtgtgttta
     2761 cattttcggg tcatgcagaa aaaagtggtg ataagagtac tctgcgtttc attattgaag
     2821 cagccaggaa gaaattactt gttgcatgga ttttatgtac tcttttgttc tttctatttg
     2881 tgtacataat agcgtggaaa cactttgggg aagattcaca taaagcatca ctttatatgg
     2941 agttattgta tgagcggatt ggtgacaaat ctttctcatt tggtgattat tgtggcatag
     3001 catttgtact acttttcccc tatgcttgtc agataattaa tcgccgtctt ataagaccaa
     3061 aactatcatc atggcttcgt cgttttcgtg tgtctcagac ctcggatact atgagtgata
     3121 ttcgtgttga gaaaggtaaa tattcgccta
//
LOCUS       DAZGBC010000303         3147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGBC010000303 DAZGBC010000000
VERSION     DAZGBC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.305"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..242
                     /locus_tag="ACHK1J_005022"
     CDS             <1..242
                     /locus_tag="ACHK1J_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CesD/SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HFO7914155.1"
                     /translation="KDYQKAFDMYHICLMLSPDNDFSLVYYMGQCQMGLKNIKMATEL
                     FNTVVTYSQNEKVKEMATAYLELLTANTEEEVQTE"
     gene            252..434
                     /locus_tag="ACHK1J_005023"
     CDS             252..434
                     /locus_tag="ACHK1J_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914156.1"
                     /translation="MKINGKNASFTPEELARKYHEEAPVDLKNNHGDKINEKNNNKHI
                     TYFPVDAILDGNKHLP"
     gene            490..2271
                     /locus_tag="ACHK1J_005024"
     CDS             490..2271
                     /locus_tag="ACHK1J_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HFO7914157.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAIQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     ATRLHNNLESTLLRHEMAHNKFRELSDAYSSSLDDAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARKYTTTTKVLKIVNECVTVATIAMSAILIVVG
                     LLAAVPSGGSSIAGALALIGGIAGAVVLGVDITCQIALGTTATGWILGKVVEGLSAAI
                     KTVDPTLLAITALLDVIGVDQDTIELVKSIYASAAASIVMATVMIGAAVICSVAIGAV
                     VSALSKTAAEEVTKEITSTIKSTIESIINSVSKNIIKVLDSVCSMIQTSVVVLKLIAK
                     ISNGLEKIGLLICETATSIMNYCVAENSADMAILQQDMSNLSKTREQMLSVLQRVDKT
                     VEQEVSQMVRVLQHRTEALKFASHSIV"
     gene            2284..>3147
                     /locus_tag="ACHK1J_005025"
     CDS             2284..>3147
                     /locus_tag="ACHK1J_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914158.1"
                     /translation="MATYEIKNATSTLNINNLDKTIFDREHSKENNIPNDNKIVQYYN
                     LKDIKHDISSQFIILKNTDREVCPELRVKLLDNLNYSRNTLDNSKSDVDKATITSSQS
                     SCSTEQTTIKISKFNILDMLDNLTMRQQVVMEEFLRVFTSMYKASAMSEAIMSNVFEQ
                     LVQYQRKETERATAASSVGVIIGSGVGAAGAAASVVANYRSHPEKMVTLTPDQEHTIN
                     RTDDDVQRASKREVREQQASLKQIDEKIKNTTKDTSLNEEQKEEALKSYDKEKETTLE
                     KIRDTNEKISTK"
BASE COUNT         1148 a          586 c          625 g          788 t
ORIGIN      
        1 agaaagatta tcaaaaggca tttgacatgt atcatatctg tttaatgcta tctcccgata
       61 acgatttctc tttggtttat tatatgggtc aatgccagat gggcttaaaa aacattaaaa
      121 tggccacgga gttatttaat acagtcgtaa cctacagtca aaatgaaaaa gttaaagaga
      181 tggctactgc ttatcttgaa cttttgacag ccaacaccga agaggaagtt caaacagagt
      241 gagtcagcaa aatgaagata aacggaaaaa acgcttcttt tacgcctgaa gagctagcca
      301 ggaaatatca tgaggaagcc ccggttgatt taaaaaataa ccatggcgat aaaataaatg
      361 agaagaataa caataaacac attacttact ttcccgttga tgccatttta gatgggaata
      421 aacatttgcc atgagttaaa taaaaaagaa ttaattacac ccggtgatag cttaaaaagg
      481 aggtgaatta tgtcggcacc gattacggga caaacgatca cgtttgaaca aatcagcgaa
      541 acattgcgta cacagtatag cgatgcggaa aaaagactgc aggacagcag caaaacgcaa
      601 gttgatccca tgcggcttaa taaaaacccg aaatcccttg ataacgatat tcgcgctcgt
      661 cttgaaaaca aaccgatgct tgctccgcct gcaatacagg tttcagattc agacaacgcc
      721 actacagcaa agacgaatga tgcccgcctg acaatgattt tgggtaattt aacaggcatt
      781 gccgatcagg atattgcaac acgattgcat aataaccttg aaagcaccct gcttcgacac
      841 gaaatggcac ataataagtt tcgtgaatta agtgacgctt actcctcatc attagatgat
      901 gcgcaaaaag ccgacgatat catgcatcag gccaataata attacaatgc ggtcgataaa
      961 aaggtgcaat cgctggagaa aaaagtcaac acattgaatc aagagttgtc acaactgcaa
     1021 cctggcgatc cgcaatataa taaagtactg acgcaaaaaa atgcggctga aaaaacactc
     1081 acgctgtcat tacaaaaaaa atcgttagct gagaaatcgt taaatacagc cattatggat
     1141 gctgatgctg cgatcggcca aagtatggaa atttttgatg aaattcaaca gcaagaacag
     1201 attaataact tcaccaccaa tatttgcctg acccaagaaa accagaaaaa tagaaacgcg
     1261 acagccacat ttatcctttt gattacctca gtaatggaag ttattggtga taccaattgc
     1321 gactctatta aaaatcaatc tgaggtaatg aaagagatta accatgtcag ggaaaataaa
     1381 ctcaatgaaa cagcgcgtaa atatactacc acgactaaag ttttaaaaat cgttaatgag
     1441 tgtgtaactg tagctaccat tgccatgagt gcaatcttaa ttgttgttgg tcttcttgct
     1501 gcagttccta gtggtggttc aagtattgcg ggtgcactgg cgcttattgg tggaatagcg
     1561 ggcgcagtgg tattaggtgt tgatatcact tgtcaaatcg ctctaggcac cacggctacc
     1621 ggctggattt tagggaaagt tgtagaaggt ctttcggctg ctattaaaac agttgatccc
     1681 actctgctcg caatcaccgc gcttctggac gttattggtg tcgatcaaga tacgattgaa
     1741 ctggttaaaa gtatttacgc aagtgcagcc gcttctattg tcatggcgac agtaatgatt
     1801 ggtgcagctg taatatgttc tgtagcaata ggggccgttg tttctgcatt atcaaaaaca
     1861 gccgcagaag aagtcaccaa agaaataaca agcactataa aatcaaccat tgaatctatt
     1921 attaattccg tttcaaaaaa tattataaag gtattagaca gcgtatgcag tatgatacaa
     1981 acatcagtcg tagttttgaa gttgattgcc aagataagca atggcctgga gaaaataggc
     2041 ttacttatct gtgaaacagc aacatcgata atgaattact gtgttgctga aaactctgcc
     2101 gacatggcaa ttttacaaca ggacatgagt aatctatcaa aaacgcgtga acaaatgctt
     2161 tcagtattgc aaagggtgga taaaaccgtc gaacaagagg taagccaaat ggtaagagta
     2221 ttacaacacc gaactgaagc cttaaaattt gcttcccatt ctatcgtata agagggttct
     2281 aacatggcca cctatgaaat taaaaatgcg accagcacac ttaatattaa caatctcgat
     2341 aaaacaatct ttgatagaga gcatagcaaa gaaaacaaca taccaaatga taataaaatt
     2401 gtgcaatact ataaccttaa agatattaag catgacataa gttctcagtt tataatatta
     2461 aaaaatacag acagagaagt ctgtccggaa ctaagagtaa aactattgga taatcttaat
     2521 tactcaagaa atacactaga taattcgaag agcgacgttg acaaagctac aattacatcc
     2581 agtcaatcaa gctgttctac agaacagacc accattaaaa tatctaaatt taatatcctt
     2641 gatatgctag ataatttgac catgcgacaa caggttgtaa tggaggaatt tttaagggta
     2701 ttcacatcaa tgtataaagc ctctgctatg tctgaggcta ttatgagtaa tgtttttgaa
     2761 caacttgtcc agtatcagag aaaagaaacc gaacgcgcca cagcagcaag ttcggtaggt
     2821 gttattatcg ggtcaggagt tggcgcagcc ggggccgcgg catctgtcgt agcaaattac
     2881 agaagccatc ccgaaaagat ggtaaccctt acacctgacc aagagcatac tatcaataga
     2941 actgatgatg atgtacagcg cgcgtcaaaa agggaagttc gagagcaaca agcttctctc
     3001 aaacaaatag atgaaaaaat aaaaaataca acgaaagata cgagtcttaa tgaagaacaa
     3061 aaagaagagg cgctgaaatc ttatgataaa gaaaaagaaa ctactctaga aaagattaga
     3121 gatacaaacg agaaaattag tacaaaa
//
LOCUS       DAZGBC010000304         3146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGBC010000304 DAZGBC010000000
VERSION     DAZGBC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.306"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2883)
                     /locus_tag="ACHK1J_005026"
     CDS             complement(<1..2883)
                     /locus_tag="ACHK1J_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7914159.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYVDTNKPIYN
                     IDTDYLTYSGYVYNTVAKDSGYYDTVFSGDTVNGVISTYYLNYDDYAAGTANTVSINN
                     STVHGMITSKYVPNFGDAAKAHDWIDGDTFTLNISNSTIDDDYEGFYFTDTYLDADGK
                     TVKSDNYEYSVAGMGTAVTLDVESNINISNNSHVAGITLEQNDTTNTAYKTGATQWDN
                     TITVNDSTVTSGSWTEDEQQDGHFGNSAEPSDYSGDGRVGYHSNDNALEFIDDSSSDY
                     RMVNNVTFNNSQLMGDVYFQSTWNNNFDYIGHPTDTDSNVYTHGGWIDDDQNVDHLTL
                     TLNHTKWVGGAYIDYAGDDIVTPDHFYDVAANSLDPNATNYDGWNRMDSAASFQSGVF
                     DVVLNDGSEWDVAKDSRIDTLAVNNSQVDVANGVELTSDTIGLNDGSVLNIGEGGQVN
                     TDHLTVNTFSTVTLANDISSDWALDALYANTITVTNGGVLDVQTSNTDAANVFTTDTL
                     ELTSGNVKDNHGNVYAGVFDINSNDYILNADLVNDRTNDTSKANYGYGVIAMNSDGHL
                     TVNGNGDINNVDTAATNQGGEADNSTADNVVAATGNYKVRIDNSTGAGAIADYAGKQL
                     IYIDDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNI
                     WNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKI
                     DGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFN
                     NDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQL
                     SNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSI
                     DNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGV"
BASE COUNT          855 a          724 c          711 g          856 t
ORIGIN      
        1 aacacccacg ttcgcggacc aatcttgatc tacgtcacca ccaccgaggt agttagcatc
       61 ggtataggcg ctgaagttct tggtgaagct gaactgagta cccagaccaa cacgtaccgc
      121 agacccttca gtaccgttat cgatggaatc accgttcaca tcgttatcgt tgttagagtc
      181 gtcgtagacg taagccagtt tgaagtacgg agtcagagcc tggtcttcgc tgtaagtgaa
      241 ggtataacct gcatctacac ccagttcata acgcatgctg tcgtaagact gaccgtcaac
      301 tttcatgtcg ttgctcagct ggtagtcatc accagactgg aacagaccag aaatgctgcc
      361 gtaaggagtc acgtaaccag catcacccag tttgaagtcg taaccggctt tcaaaccgaa
      421 gccccaagcg tcggagttgg tgctaccgtc aacgtaagta ccgttgctca tggttgcaga
      481 caggtcgttg ttgaagtgag agtagctcaa gctaccatca acaaagacgt tgttcgcgaa
      541 gtgagcagaa gagtagatgt aggcagtctg gctgtcttga tccacctgac cagaacggtc
      601 attcatgtca cctttagcga agcctgcagc cgcaccgacg atccacttag cgttgttacc
      661 gtcaattttg gtatcaacac cgaccatgat gccgttaaca tcctgatcat agttgatggt
      721 gccgttgtca ccgttgaagt taccaccgaa gtagcttacc catgcgccgc cgttatcagc
      781 caggccatga cgagagttgg tcaaacgagt accaacggtg tcttgttcca ggttccagat
      841 attggtgttc gcagacggga tgctcagcgc catgttagcg tagtcagtca gctccatctg
      901 ttgcagaaca acggtgttac cgcgctgttc agcctgatag gtgtatgcac ccaggtcagc
      961 tttgttagca gcagagaagg tcgcgttggt tttggtatca tcgatataga tgagctgctt
     1021 acctgcgtaa tcagcaatcg caccagcgcc agtggagttg tcgatacgaa ctttgtagtt
     1081 accggttgca gctacaacgt tgtctgcagt gctgttatcg gcttcaccac cctggttggt
     1141 tgcagcggta tcaacgttgt tgatatcacc attaccatta acagtcaggt gaccgtcaga
     1201 gttcattgcg attacaccgt aaccgtagtt agctttgctg gtgtcgttgg tacgatcgtt
     1261 gaccagatcc gcattcaaga tgtagtcgtt gctgttgata tcgaacacac cagcataaac
     1321 attaccatgg ttgtctttca cgttaccgct ggtcagttcc agagtatcgg tggtgaagac
     1381 gtttgcagcg tcagtattac tggtctgcac atccaataca ccgccgttag ttacggtgat
     1441 ggtgttggcg tacagtgcgt cgagtgccca atcgctgcta atatcattag ctaaggtgac
     1501 ggtactgaag gtgttaacag tcaagtgatc tgtattaacc tgaccacctt caccgatgtt
     1561 caaaacagaa ccgtcattca ggccgatagt gtcagacgtc agctcaacac cgttagcaac
     1621 atcaacctga gagttgttta cagccagggt atcaatgcga gagtctttcg ccacatccca
     1681 ttctgaacca tcgttcagaa caacatcaaa cacgccgctc tggaaagaag ctgcgctgtc
     1741 catacggttc caaccatcat agttagtagc gtttggatcc aagctgttag ccgcaacgtc
     1801 atagaaatga tccggagtta cgatgtcatc accagcataa tcaatatacg caccaccgac
     1861 ccatttagta tggttcaggg tcagggtcag atgatcgacg ttctgatcat catcaatcca
     1921 tccaccgtgg gtatagacgt tggaatctgt atcagttgga tgaccgatgt aatcaaagtt
     1981 attattccag gtactctgga aataaacatc acccatcaac tgagagttgt tgaaggtaac
     2041 attgttgacc atgcggtaat cagaagaact gtcatcaatg aactctaaag cgttatcatt
     2101 gctgtggtaa ccgacacggc catcaccaga gtaatcactc ggctcagcag aattaccaaa
     2161 gtgaccatcc tgctgctcgt cttcggtcca agaaccagaa gttacagttg agtcattaac
     2221 agtgatggtg ttgtcccact gagttgcgcc agttttgtac gcagtgttag tggtatcgtt
     2281 ctgttccagc gtaatacctg caacatgaga gttgttgctg atattaatgt tgctttcaac
     2341 atccagcgtt actgcggtac ccatgccagc aactgaatat tcatagttgt ctgacttaac
     2401 agttttacca tcagcatcca aataggtgtc ggtgaagtaa aaaccttcat aatcatcatc
     2461 aatcgttgaa ttgctgatgt tcagagtgaa ggtgtcaccg tcaatccagt cgtgagcttt
     2521 tgcagcgtcg ccgaagttag gaacatattt tgacgtgatc ataccgtgaa cagttgagtt
     2581 attaatactc acagtatttg cagtacctgc tgcatagtca tcatagttca ggtaataggt
     2641 agaaattaca ccatttacag tgtcacctga gaaaacggtg tcatagtaac cgctatcttt
     2701 agctactgtg ttataaacat aaccactgta ggtcaggtaa tcggtatcaa tgttgtaaat
     2761 tggtttgttg gtatcaacat aaccaaaagt attataagta gcagcattag cctgagaggt
     2821 gatagccaga gtgcaagcca atgctaattg tgatactaca agtttctttt tccaggagtg
     2881 catttgtcat ccctcctcag ggacgtaata agttgatcca tcaattatca atgcatagaa
     2941 aacatcttat gtttcgcgtt tacgattatg cggcgttata gaggaatggt tcaattattc
     3001 tttcccctaa gtccgattgc ggacatatat aaaaatatct atacgcacaa agaataagtt
     3061 gacaaaaaca ctgtatttca acacgttaat gaaatcatta attttgaatt atagatcttg
     3121 aaaataccaa aacaacaaat cagaca
//
LOCUS       DAZGBC010000305         3145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGBC010000305 DAZGBC010000000
VERSION     DAZGBC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.307"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..148
                     /locus_tag="ACHK1J_005027"
                     /pseudo
     CDS             1..148
                     /locus_tag="ACHK1J_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            607..750
                     /locus_tag="ACHK1J_005028"
                     /pseudo
     CDS             607..750
                     /locus_tag="ACHK1J_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            822..1094
                     /locus_tag="ACHK1J_005029"
     CDS             822..1094
                     /locus_tag="ACHK1J_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914160.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFADFIAGHSSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            1075..2281
                     /locus_tag="ACHK1J_005030"
                     /pseudo
     CDS             1075..2281
                     /locus_tag="ACHK1J_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            2744..2968
                     /locus_tag="ACHK1J_005031"
     CDS             2744..2968
                     /locus_tag="ACHK1J_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7914161.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            2965..>3145
                     /locus_tag="ACHK1J_005032"
     CDS             2965..>3145
                     /locus_tag="ACHK1J_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO7914162.1"
                     /translation="MKFVSSQEVIEFHDRLISRDGGVAGMTEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLV"
BASE COUNT          807 a          770 c          866 g          702 t
ORIGIN      
        1 tgctcgattt cagtgaccat ccgcttatgc ctccacactc tgcacaactg tttgctgaac
       61 accttcccgt cgagtggata cagcactgcc tgacgcgtcc tgctcatgag accgttcggt
      121 tcgccgtcgt cgtttaccgg gggacatggg atccgcttgg aattcggaaa tttccccacg
      181 ctaagagaat tgagttgaac aagtgcttac atcccggcaa gctaactcac tgaatgtcaa
      241 ggctatacga aatgttctga ccgtctgata acgaccgggc cccgtgctga cacgggacat
      301 tgaacgggtg ctagagcgaa gcggggaagc cccggtctac ggaccaaaat gaaaaacagg
      361 gcttctggag aaaatggttc cattaaatag ctgcgccgaa tagtagatca ctttgaggga
      421 actcagcccg gattgcgcga tctgatcaat tgccaaacca atcaaaatca ccaaccggac
      481 tgagcgatgc cgatcatagc accaattccc cgtgacgaac tgtcacgaac ggtgcaatag
      541 tgatccacac ccaacgcctg aaatcagacc tagggggtaa tctgctctcc tgattcagga
      601 gagtttatgg tcacttttga aacagttatg gaaattaaaa tcctgcacaa gcagggaatg
      661 agtagccggg cgattgccag agaactgggg atctcccgca atacggttaa acgttatttg
      721 caggcaaaat ctgagccgcc agaaccacgg cagcctggcg gctgcaaatc ggggtgtggc
      781 agaatatgag ctttcagagt aaccatccag aatgagttcc catgtcacac aacctggagc
      841 accagaaagt ccacaccaga atggtaaaag aggttctgaa agccgtcgcc agagcaaata
      901 atcaccctta tcagtccgta tttgcagact ttatcgccgg tcattcctcc tgcacggtct
      961 gtttctggga aaccttccac aaaatgtacc cggattcacc gtacgaatac gtcacgttct
     1021 gccatacctg ccgtcgtttt gatttatacg aaacagaagc ggagatgaag gcggatgacc
     1081 cgaagtggtg gtgattttca gccccgaccg ctgaaacgcc tgttcacagc caaccagtgc
     1141 tggacgtcct tcctggatgc gggcggtctg cgcgatatcg aagtggaagc cgtcaccaaa
     1201 atgctggcct gcgacacgcg gataccgggc gtgaaggaat acaactgcga taaacctgac
     1261 tgcccccacg tcagatacgt cactaactca tgcggctgcc gtgcctgccc gtcctgcgga
     1321 aagaaggcca cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg
     1381 gtacacctgg tcttcaccct gccggacacg ctgtgccggt gttcgaaagc aaccgttggc
     1441 tgctgaatga cgtgtgccgt ctggcggtgg agaatctgct gtatgccgcc cgaaaacggg
     1501 ggctggaaac cggtatcttc tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc
     1561 cgcatgtaca tatgtctgta acctgtggag gtctgaataa gcatggtcag tggaaaaagc
     1621 tgagcttcct gaaagacgcg atgcgttcgc ggtgaatgcg gaatatgcgg cagctgcttc
     1681 tgaaagcgtg gtctgagggg ctggcgatgc cggagtcgtt gtcacatatc acgacggaat
     1741 cacagtggag aagcctagtg ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga
     1801 agaaaacggc cggcgggagg aatacggcgc gctacctggg tcgttatctg aagaagccgc
     1861 cgatagcagc ctcccggctg gcacattaca acggaggggc gagcctgagc ttccgttacc
     1921 tggaccacaa aacgggagaa acggcgacgg aaacgctgac acaacatgag ctggtcgcga
     1981 ggctgaaaca gcatatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg
     2041 ccaaccgggt gtgtggagag aagctgccgc aggtgtaccg tgcactgggg atggataaac
     2101 cggaagcagt ggcgaaagtg tgctatgaac aaatggtgaa acagttcctg agtcgtgacc
     2161 cgttcgaatg cgtgctgtgt gcggccggat ggtataccgc caggcccttg cggggctgaa
     2221 tgtatcaggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
     2281 acgcaggcta agtgtgcccg ggaattggtg caggaataaa aaacagcctg tcgcggtata
     2341 aaaaccgccc atacacagag agaaaacacc ggagtggagg gaaaagcaga agagctcacg
     2401 tggcataagg cgtggacagc ggtatcgtct ggtttttcaa ttttgttttt caatttccta
     2461 tacgtgaact tcacttttcc ttagcgtgta ttatcgcaga aatgcccacg gaacccttac
     2521 aatattgtac gtttcccaaa cgtagcccat caggcgcgga actcatcgag gcgcgggaat
     2581 aaaatcccat tcagacgcag gatagattca agcaagctca cttatcgtca aaatcggtgt
     2641 tgcaaaaacg ggagtgacca tagattcgtt tcccaaaccg ccccctttta tattgaccag
     2701 aagtgttgta cagtttaatt gtacaactca aagaggaagc actatgttta catgtaacta
     2761 tagccatgct cgcgataacc tggcctcgtt gctcgagctg gcggttgatg aaaatcagcc
     2821 tgttgagatt cagcgccgta aaggtggcgc ggctgtacta gttgataaag acgcttttga
     2881 acgataccgt aaagcggctc ttgatgctga gtttgctgag atcatgcaaa gtcatgggac
     2941 ggcctatacg gagctttcta agcgatgaaa ttcgttagtt cacaggaagt gattgagttt
     3001 catgaccgct tgatttcgcg tgatggtggc gtggcgggta tgacagaacc cggtcgcgct
     3061 gacgcgatca ttcatcgtgt tttgaacatg tatcattatg aaggtgtgac agatattatg
     3121 gatctggctg ccgtatattt agttg
//
LOCUS       DAZGBC010000306         3137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGBC010000306 DAZGBC010000000
VERSION     DAZGBC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.308"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..239
                     /locus_tag="ACHK1J_005033"
     CDS             54..239
                     /locus_tag="ACHK1J_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7914163.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPATVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            325..549
                     /locus_tag="ACHK1J_005034"
     CDS             325..549
                     /locus_tag="ACHK1J_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914164.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1004..1513
                     /locus_tag="ACHK1J_005035"
     CDS             1004..1513
                     /locus_tag="ACHK1J_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7914165.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            1485..>3137
                     /locus_tag="ACHK1J_005036"
     CDS             1485..>3137
                     /locus_tag="ACHK1J_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7914166.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVAGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVESTIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPE"
BASE COUNT          784 a          720 c          877 g          756 t
ORIGIN      
        1 gcgccagctc gatgttgctg cgctcgatgc aaaatactcg agggaattag ccgatgcgag
       61 agctgaaaat gaaactctgc gtgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa
      121 cgccacctgc cacggtaccg tgcgtgaagc caccagcacc tccggcgtgg ataatgcaac
      181 cggcccccga ctggcagaca ccgctgaacg ggattatttc atcctcagag aacggttgat
      241 gacaatgcag aagcagctgg aaggggcgaa ggaatatatc cgcactcagt gtattaacta
      301 gtatttttgt tatccggaga atgcatgaag aaattactgg taaccgtaga accttttcag
      361 ggaacaattc cgttccgtat tttgcagcgt ggtcgtgttc ttgttgaagg ttcgttcagt
      421 ggtaaatgta cgcaattaca ctcccggacc tttcaggtga atgccacgaa tgaagagcta
      481 accgttgagt gtacgatgaa tgcagctaaa tgccgcatgg tatcggctgc attacagcca
      541 gtgtgttgag cgaccttatt atccatgcgc ggtattgtcg ccgtattcct gcattaacag
      601 agaccgcagc ccgacaggga gactcctctg cgcgagtgtg cggggataat caaaaacgat
      661 acacaccggg gtttaccgcg ttaacggagc gcggagttgt cccctcatgg tcgctggtcc
      721 ggtgcgatgg tggaagaaac tggattttgt tgcaactgat aaccattatc attttagcgg
      781 gtcctttccg gcgatccggg ccgttacggg gcggcgacct cgcgcgtttt cactatttat
      841 gaaaattttc cgggatccat gtccggtttc tctgcaagtt aactatatga aaaatataaa
      901 aacaggtctt ctgtgaaccg gacatgcgca aaaaatgggc atgtgaaccg gacatgacct
      961 gttttgttgt gattgtgagg tgagagtttt tgcgaggtga ggagtggcta cgcagactga
     1021 agttgccagg catttaagtc tgaccgatcg ccagcttcgc agattgcaga aattgccggg
     1081 tgccccgata tcgaataagc gagggcaact ggatctggat gcctggcgtg atttttacat
     1141 atcgtatctg aggagaagta aaaacgatgt gcctgatggc gatagcgaag acgactatga
     1201 ggagaaattg cttattgcca gatgggaact gacagcggaa caggctgtta cacagcagtt
     1261 aaaaaatgag gtgtcaaaag gaaaacttat tgacaccggg ttctgtattt ttgccctcag
     1321 taagctggca atggcgttat ccagtacgct tgattccatc cctttatcca tgcagcgaca
     1381 gtttcctgat ttaacaccgc gccatcttga ccatctgaaa acccttattg cgaagggggc
     1441 aaatcagtgt gcgcgggcgg gggataaatt accggattta ctcgatgaat atatcagagc
     1501 aacaactgaa taatatgatg agcgctgtca caacagcatt acagcccctg ataagggcat
     1561 tgccggtgac gccagttgaa tgggctgatc aaaattatta tctgcctaaa gaatcttcat
     1621 atggtgaggg agaatggaaa acgctgccgt tccagatcgc catcatgaac agtatgggga
     1681 atgatcagat ccgcactgtt aatctgatta aatctgcccg tgttggctat acaaagatgt
     1741 tgctgggagt cgccgggtat tttattgagc ataaatcccg aaacagtctg ctttttcagc
     1801 ctacggattc tgccgctgaa gattttatga aatctcacgt ggaatccacg attcgcgatg
     1861 ttccctgcct gaaaaaactt tctccctggc tgggacgtaa acatcgtgat aataccctca
     1921 cgctgaaacg cttttcatcg ggtgtgggtt tctggtgcct gggtggtgcg gcagcaaaaa
     1981 actaccgtga aaaatctgtg gatgtggtct gctatgacga actttcctcg ttcgagccgg
     2041 atgttgaaaa agagggctcg ccaaccctgc tgggggataa acgtattgag ggctcggtat
     2101 ggccaaaatc cattcgcggc tcgacgccta aaataaaagg ttcctgccag atcgaaaaag
     2161 cggccaacga gtcggcgcat ttcatgcgtt tttatgtgcc ctgcccgcac tgtggggagg
     2221 agcagtatct gaaatttggc gatgagtcca cgccttttgg gcttaaatgg gagaaggaca
     2281 gccccgaaag cgttttctac ctctgtgaac atcatggctg cgtgatccat cagtctgagc
     2341 ttgaccagag caacgggcgg tggatctgtg aaaacacggg gatgtggacc cgtgatggtc
     2401 tgacgttttt cagcgctgcg ggtaatgaaa ttccgccgcc gcgctccatc acgttccata
     2461 tctggacggc gtacagtccg ttcaccacct gggtacagat agtctatgac tggctggatg
     2521 cactgaaaga tcccaacggc gtgaaaacct ttgtgaacac cacgctgggc gagacctggg
     2581 aagaggccgt gggcgaaaaa ctcgatcacc aggtactgat ggataaggtt gtgcgttaca
     2641 cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg cagcgaaacc
     2701 gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg gtggataaaa
     2761 tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat gcggcgatca
     2821 acaaaaaata ccgccatgca gacggaaccg aaatgaccat ttcccgggtc tgctgggaca
     2881 ccggggggat cgatggcgaa atcgtttatc agcggtcaaa aaaacacggt gttttccggg
     2941 tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg ccaaaaaccc
     3001 gcaatcagcg gggcgtgtat ctgtgtgaag tggggacgga cactgcaaaa gaaattctct
     3061 atgcccgtat gaaagccgat cccacgcctg cggatgaggc cacgtcgtat gccatccgtt
     3121 ttcctgatga tccggag
//
LOCUS       DAZGBC010000307         3130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGBC010000307 DAZGBC010000000
VERSION     DAZGBC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.309"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..367)
                     /locus_tag="ACHK1J_005037"
     CDS             complement(44..367)
                     /locus_tag="ACHK1J_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914167.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(400..1302)
                     /locus_tag="ACHK1J_005038"
     CDS             complement(400..1302)
                     /locus_tag="ACHK1J_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO7914168.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRNNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGRDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            2617..>3130
                     /locus_tag="ACHK1J_005039"
     CDS             2617..>3130
                     /locus_tag="ACHK1J_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914169.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEEIKNN
                     NPELFPHGFDI"
BASE COUNT          938 a          728 c          562 g          902 t
ORIGIN      
        1 tataagcctg tatattttta aatcacaaaa gatgaatgtc aagttattca acatcaatcc
       61 aaagaggaat attatgtatt cccttaacgg gttctcccag aggaaaaaca caggtccctt
      121 taatcagtat ttttcttccc gaccatctga catcacagtt aaaatctccc accacatcaa
      181 aataatccgc aatggattca tagatgtccc attcatcata gatatcccat ttatctgctg
      241 ttccttcaca ttcaaactcg gcctgatatc cggaatcaac atatcgcgcc agatagtgtg
      301 accacaaagg cgaatttttt gtaatctctt ttacagagat tattttcagc aaaatagtac
      361 aatccataat atttacccgt acgaaatcgc catgagttat tactcatagc ctaacaactg
      421 acaataaacc tgttcaatat atcgtcctct gccatcacca tgaccaagat caattgatgt
      481 cagtgcaagt gcctccttat aactgtaacc gttcataata tgatatttca tggcacttgc
      541 agcgtaactg taccgaaaac tgtgtgcagt ctcatctctg cctttcagtt ctccataacg
      601 acgtaatgta ttaagataat aatccattgc cgacttaaga ttatctctgt ctataagacg
      661 tccattatgt tcactggcat atttgagtgc ataattaaca gcatcaagaa cgttatcccg
      721 gtcaacaacc gttgtcattc tttcccgtcc ccctttcgta ccaaaaatca ctcttatttt
      781 ttcatctccc cgttgtaacg cttttttcca ggttcgtaat gatttggccg actggatagc
      841 ttcttcattt ctcagaccga ggtaacgtga cagtttaaca acagcagcca cccctttatc
      901 ttttgcttcc acacgctgaa gaatctcccg aaaacgctca tccgtgatta atttttttgt
      961 tcccttacga ctggagccgg aaatgcctaa cgctttattg ctgagacgag gatgttcggg
     1021 atcagccatc ttatatttac ctgcctgacg taacataacc cgtattgcag acatttcatt
     1081 ttcccttgtt cgcccagaaa cattattccg gcttctcata tatagctcaa tatgccggac
     1141 ttttacctgc gcggcagaac tgatctgtac attcattttt gccagacttt cagcaaagcg
     1201 gtgcatgatc ctggtgtaat cactcagctt cttgaagctg cctttgtctt ttcgaatgtg
     1261 agcaaccaga tcctgtttca gtaatttttc acgtctggac atcaacttct cctgcacata
     1321 ataattttta ccttaaacag ctgtacgttc ggcagatgag tttttctgtt gtgccgtgaa
     1381 cacaacagaa aaactcatct gcatttctcc ctgacgacac ggttaaacgt tgcgatcctt
     1441 cgggtagtga ctgcctgtgc gccagggtag cgcagattgt ttaggtattg aggggtaaca
     1501 aatcggtgtt gcacaccgac tgcagcattt gctgtacctg tctccaggtc gcccccgaca
     1561 ccacctctaa taagtggtat cgccgattcg gcatctcact acataaaact ccttaaaaaa
     1621 acaattgatt ctgcaaaaaa ccatttatgg acgttattca caaaatcgtc cgtaaatttg
     1681 cagaagaggt tgaaaaaaca ctctcacgcc gggtcacttc ttcaaatgtt aaaaaacatc
     1741 acgtgaaacg gcagttcgtg cgggttcgtt aagcagacgc gcgtgcgtca ctttgtcatc
     1801 gtgacacgct gacgaaagtg acgccgcgca gcttcagacg ccagatacac gcgtaagcga
     1861 ccaaatttca aatggtctta tacagcgcgc acagtcgcag gaagtgctgg caaacaaatg
     1921 aaaagaataa aagaagtcaa taccgccatt acgcagtaag aacattccgg catcaccgga
     1981 tggataagga tcacacaacc actgctgtgt attgcccgta ataaagggcg cggttaaaag
     2041 aaaccttcat cgtcatgact gacgatatct gctctcaaag gtgggcagat ttaccaccac
     2101 cagaaacatc ctggctgcac atcccctttc aggttacggg ggtgttgatc acccgaaggc
     2161 gttcctctca gatcaacgga acattggtgc ccaccgaagc gggatgtggt cacccgaagg
     2221 cgctcctctc agaccactta tggagcattg gtggtcgccg aagcgacaga taatgcattc
     2281 tgagcatatc agagcgcata aaaggcgtca attatccatg ctccctcacg aaacagaagc
     2341 tgaactctta acacctgcta tcggaactca ctgaataaac aactaaaaag gacattgtga
     2401 aaattaacat caacagtatg taaagtgatt tgttttgtct taaatgggtg aatgtaaaaa
     2461 aatattctat acaatccaca catccttgta tcatatatca agtaaacatc cacagagata
     2521 acagcttatt tcaaataagc agattctgtt tgatacttca gaaaaatagt atatgctcct
     2581 gatttactga tgttataagt tatacaggat agttaaatgt gtgattttat aaaatcatgt
     2641 tatgcagcac caagaattaa caaaatggct gattttttct catcaacagc atctggagta
     2701 aaacataaac ctttgaagca tgtaaaaatt gataacaggc ttttcccatc tcctgagtat
     2761 ataatttttt ctgactatct tacgaaaaac agtggtccat ttgttaacca ctacttttgc
     2821 agtattccat acagcaaaga agaagaatgc aggctgggtg tgactattat gaaatatgca
     2881 agccatcgaa ataagccatt gagcttatgg tgtcttggaa tggcagaagg agccatggcc
     2941 agaacaattt ctcaactatc aaaaggacag gttttatcat taacaaccag cccaacaaaa
     3001 gaaaatgaac ctgtattctt tagtcatggt tctcatattc actcttactt tatttgctcc
     3061 ccattctttc ttgttcatga agaaataaaa aataacaacc ctgaactatt tcctcatggt
     3121 tttgatatta
//
LOCUS       DAZGBC010000308         3107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGBC010000308 DAZGBC010000000
VERSION     DAZGBC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.310"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2852
                     /locus_tag="ACHK1J_005040"
     CDS             <1..2852
                     /locus_tag="ACHK1J_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7914170.1"
                     /translation="FNGGGTINLEQDLHTGSGGLIFDDSSVYEINGNNYSYKGAGINT
                     GNSSVVNWNVKGIKGNDLHKIGKGTLNIKSTQDGNLKIGDGKVVLQAEKSFDNIYITS
                     GKATVVIDKNNALNNENEFSGFYFSKNGGVLDLNGHDQSFKKIAATDNGATITNTSEK
                     TATLSLSSTSDYLYHGNISNNIQIRHEHTGKKSDSRLILDGDININNDIHIKNAQVVM
                     QGHATSHTVVKNQACGLPSFLCPVTTTAHLSNLEKNDALKNGVAYKANNQSASFEQPD
                     WEDRQFSFRTMTLDNSVFTTSRNSFLKGDIISSDSDIYFGSKDDVYIDTLAGENITGN
                     GFSFSQNIKKGVSVGDSGFSGGIDSTDGSIRIGDKANVTLTKSSSMDNTTLTIEKGGL
                     INAQGGLFTSKESLIAGQMNLTGKPVLNTNAWSPSMYLGFGGYKLTEDGAQLSARNQA
                     SVIADIYSDKTANISLGKENNTESKNTPAYSSFAISLLNGFDTSLEGKINAAKSTLTM
                     NDALWKVTGDSALKNMSTTGSMVLFSNENRNSFNKLTVDELTTNNSAFVMRTNTQQTD
                     QLIVKNKLEGANNLLLVDFIEKKGNDKNGLNIDLVKAPENTSKDVFKTETQTIGFSDV
                     TPEIKQQVKDNNSVWTLTGYKTVANASAAAKATSLMSGNYKAFLNEVNNLNKRMGDLR
                     DINGEAGAWARIMSGAGSAGSGYSDNYTHVQIGADKKHELDGLDLFTGLTMTYTDSHA
                     GNHDFSGETKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSW
                     YAGAEAGYRYHVTEDAWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDKDYNPLIGRTG
                     VDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLN
                     AEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT          964 a          668 c          688 g          787 t
ORIGIN      
        1 cttttaacgg tggtggaact ataaatcttg aacaggattt gcatacaggt tctggagggc
       61 ttatttttga tgattccagt gtatatgaaa taaacggaaa taattactca tacaaaggtg
      121 ctggtattaa caccggaaat agctccgttg ttaactggaa tgtaaaagga attaaaggta
      181 atgatctcca caaaataggt aagggcacac ttaatataaa atcaacgcag gatgggaatt
      241 taaagatcgg tgacggcaaa gttgttttgc aggctgaaaa atcatttgac aacatttata
      301 taaccagcgg aaaagcaaca gtcgttatcg ataaaaacaa tgcattaaat aatgaaaatg
      361 agttcagcgg gttctacttt tctaaaaatg gaggagtgct ggatctgaat ggacacgatc
      421 aatcctttaa aaaaatagct gcaaccgaca atggtgctac aataacaaac acatcagaaa
      481 aaacagccac cttatcttta tccagtacat ccgactatct ttaccatggg aatatcagca
      541 ataatattca gatacgccat gaacatacag gtaagaagag tgattcacga ctcattcttg
      601 acggagatat aaatataaat aatgacatac atattaaaaa tgcgcaggtt gtgatgcagg
      661 gacatgccac atcccatact gttgtaaaaa atcaggcatg tggtttacca tcttttttat
      721 gtccggttac aacaaccgcc cacttatcca atcttgaaaa aaatgatgca ttaaaaaatg
      781 gggttgcgta taaagcaaat aaccagtcag catcatttga acaaccagac tgggaagatc
      841 gccagttctc attcagaacc atgactctgg ataattcggt ttttacaacc tctcgtaact
      901 cttttctgaa aggagatatt atctcatcag actctgatat ctattttgga agcaaggatg
      961 atgtatatat tgacactctc gctggtgaga acatcaccgg gaatggattc agttttagcc
     1021 agaatattaa aaaaggcgta tctgtgggtg attccggttt tagcgggggg attgactcta
     1081 ctgatggtag tatcaggatt ggtgataaag caaatgtcac cctgactaaa tccagttcaa
     1141 tggacaatac aactctgact atcgagaaag gtggtttaat aaatgctcag ggtggcctgt
     1201 tcaccagtaa agagtcactg attgcgggac agatgaacct gacaggtaaa cctgttctga
     1261 atacaaacgc ctggtctcca tccatgtatt taggctttgg tggctacaaa ctgactgaag
     1321 atggggctca actctcggcc agaaaccagg catctgtaat tgctgatatt tactctgata
     1381 aaacagcaaa tatctcactt ggtaaggaaa acaatactga aagtaagaac actcctgctt
     1441 actcatcatt tgccatatca ttattgaatg gatttgacac ctcactggaa ggaaaaatta
     1501 acgcagccaa atccacactc acaatgaacg atgcgttgtg gaaagtgacc ggtgattctg
     1561 ctctgaaaaa catgtcaaca accggcagta tggttctctt cagtaatgaa aaccgtaact
     1621 cattcaataa gctgactgtt gatgaactga ccaccaacaa cagcgccttt gtgatgcgaa
     1681 ctaatacaca acagaccgac cagttaattg ttaaaaacaa actggaaggt gcaaacaacc
     1741 tgctgttagt cgatttcatt gagaaaaaag gaaacgacaa aaacggtctg aacatcgacc
     1801 tagttaaggc tccggagaat accagtaaag atgtattcaa aactgaaaca cagaccattg
     1861 gtttcagtga tgtgacgcct gaaatcaaac aacaggtaaa agacaataac tccgtatgga
     1921 cgctgactgg ctataagact gtcgccaatg cgagtgctgc agcaaaagca acatctctga
     1981 tgtccggcaa ctataaggcc ttcctgaatg aggtcaacaa cctcaacaaa cgtatgggtg
     2041 acctccgtga catcaacggt gaagccggtg catgggcacg tatcatgagc ggcgccggct
     2101 ctgccggtag cggttactct gataactaca cccatgtaca gattggtgcg gataaaaaac
     2161 atgaacttga tggccttgac ctcttcaccg gtctgaccat gacctatacc gacagccacg
     2221 ccggaaacca tgatttctcc ggtgaaacca aatctgtcgg ggcaggtctc tacgcttctg
     2281 ccatgtttga ttccggtgcc tatatcgacc tgattggtaa gtatgttcac catgataatg
     2341 agtacaccgc aacctttgcc ggactcggaa cccgtgatta cagcacacat tcatggtatg
     2401 ccggtgcaga agcgggctac cgctatcatg tcactgagga cgcctggatt gagccacagg
     2461 cagaactggt ttacggtgcc gtatccggta aacagtttgc atggaaggac caggggatgc
     2521 atctgtccat gaaggacaaa gactacaatc cgctgattgg tcgtaccggt gtggatgtgg
     2581 gtaaatcctt ctcaggtaag gactggaaag tgacagcccg tgccggtctg ggctaccagt
     2641 tcgacctgct ggctaacggc gaaaccgtat tgcgggacgc atcaggtgaa aaacgtatca
     2701 aaggtgaaaa agacagccgt atgctgatgt ccgttggcct gaatgcagaa atcagggaca
     2761 acgtccgctt tggactggag tttgagaaat ccgcctttgg taagtacaac gttgataatg
     2821 ctgtcaacgc aaacttccgt tactcgttct gatactccgt attcaaaagg gcctgcgggc
     2881 cctttatgct caggacctgc cgtcctggta tacatttccg gggtatcagt cgatttgttt
     2941 acttaactgc gatgactaaa gctggcagta atgccggctt ttttacgacc acacacgtct
     3001 tcctctcatc cggaactgtg atacaaatcc tgcatacagg attgatcgcc ggtgcccaca
     3061 aatggcacat tttcagtaag attctgaatg ttacattccc ccttcat
//
LOCUS       DAZGBC010000309         3095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGBC010000309 DAZGBC010000000
VERSION     DAZGBC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.311"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..1319
                     /locus_tag="ACHK1J_005041"
     CDS             join(91..406,406..1319)
                     /locus_tag="ACHK1J_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7914171.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
     gene            1326..2189
                     /locus_tag="ACHK1J_005042"
     CDS             1326..2189
                     /locus_tag="ACHK1J_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7914172.1"
                     /translation="MHPSYRMQHINDRRAGYIASTCLAGKKEERVNVSDIFIDGMRFR
                     IFVRPDISDITPCLIYYHGGCFVSGGFETHDNQLRQLAYYSGCRVIAVQYRLAPEFLF
                     PVAHDDAERGACIIWQNAESLGIDRNRISLAGDSAGGHLALVTALRLKSVAQWQPSQL
                     ILIYPMLDATARLESYAMFGDDYVITRDALVSGYDMYLHDTDREHPEASPLWRSDLQG
                     LPATHIITAEFDPLRQEGEMLYQRMTEQGVMCTCQRYLGVIHGFFQLGGISQAGRDAV
                     QAVASRLSMFS"
     gene            2422..2574
                     /locus_tag="ACHK1J_005043"
     CDS             2422..2574
                     /locus_tag="ACHK1J_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7914173.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            complement(2712..>3095)
                     /locus_tag="ACHK1J_005044"
     CDS             complement(2712..>3095)
                     /locus_tag="ACHK1J_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914174.1"
                     /translation="CLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSL
                     RQLEEHAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDN
                     FYRITPKISNNTSNQRQPNESDCHN"
BASE COUNT          771 a          690 c          826 g          808 t
ORIGIN      
        1 agactggccc cctgaatctc cagacaacca gtatcactta tttaagtgat agtcttaata
       61 ctagttttta gactagtcat tggagagcag atgattgatg tcttaggacc ggagaaacgc
      121 agacggcgta ccacacagga aaagatcgca attgttcagc agagctttga accggggatg
      181 acggtctccc tcgttgcccg gcaacatggt gtagcagcca gccagttatt tctctggcgt
      241 aagcaatacc aggaaggaag tcttactgct gtcgccgccg gagaacaggt tgttcctgcc
      301 tctgaacttg ctgccgccat gaagcagatt aaagaactcc agcgcctgct cggcaagaaa
      361 acgatggaaa atgaactcct caaagaagcc gttgaatatg gacgggcaaa aaagtggata
      421 gcgcacgcgc ccttattgcc cggggatggg gagtaagctt agtcagccgt tgtctccggg
      481 tgtcgcgtgc gcagttgcac gtcattctca gacgaaccga tgactggatg gatggccgcc
      541 gcagtcgtca cactgatgat acggatgtgc ttctccgtat acaccatgtt atcggagagc
      601 tgcccacgta tggttatcgt cgggtatggg cgctgcttcg cagacaggca gaacttgatg
      661 gtatgcctgc gatcaatgcc aaacgtgttt accggatcat gcgccagaat gcgctgttgc
      721 ttgagcgaaa acctgctgta ccgccatcga aacgggcaca tacaggcaga gtggctgtga
      781 aagaaagtaa tcagcgatgg tgctctgacg ggttcgagtt ccgctgtgat aacggagaaa
      841 aactgcgagt cacgttcgcg ctggactgct gtgaccgtga ggcactgcac tgggcggtca
      901 ctacgggcgg cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc
      961 gcttcggcaa cgagcttccg gcgtctccag tagagtggct gacggataat ggttcatgct
     1021 accgggctaa tgaaacacgg cagtttgccc ggatgttggg gcttgaaccg aagagcacgg
     1081 cggtgcggag tccggagagt aacggcatag cagagagctt cgtgaaaacg ataaagcgtg
     1141 actacatcag tgtcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg
     1201 cgttcgagca ttataacgaa tggcatccgc atagtgcact gggttatcgc tcgccacggg
     1261 aatatctacg gcagcaagcc agtaatgggt taagtgataa caggtgtctg gaaatatagg
     1321 ggcaaatcca tccttcttac cggatgcaac atattaatga cagaagagct ggttatattg
     1381 ccagcacttg tcttgcggga aaaaaagaag aacgggttaa tgtcagcgat atttttatag
     1441 acggaatgcg attccggatt tttgttcgac cagatatatc agatatcact ccatgtctta
     1501 tttattatca tggcggttgc ttcgtcagtg gtgggtttga gacccatgat aaccagctaa
     1561 gacaactggc ttattatagt ggttgtcgtg ttatcgcagt tcagtaccgt cttgctcctg
     1621 agtttttgtt tcccgtggcg cacgatgatg ctgagagagg agcctgcatc atatggcaga
     1681 atgcggaatc tctgggaatt gaccgcaaca ggatttctct ggcaggtgac agtgctggag
     1741 gtcatcttgc tcttgtaacg gcattacgcc ttaaatcagt agctcagtgg caaccttcac
     1801 aacttattct tatttatccc atgcttgatg caacagcacg cctggaaagt tatgccatgt
     1861 ttggtgatga ctatgttatt acgcgagatg cacttgtatc aggttatgat atgtacttac
     1921 atgatacgga tcgtgaacat ccagaagcct cgcctctctg gcgttcggat ttacaggggc
     1981 tgcctgctac tcatattatc acagctgaat ttgatccttt acggcaggaa ggggagatgt
     2041 tgtatcagcg aatgactgag cagggcgtaa tgtgtacgtg ccagcgatat ctgggggtta
     2101 ttcatggttt ctttcagctt ggcggtataa gtcaggctgg aagagatgca gtgcaggcag
     2161 tggcatcccg attgtcgatg ttttcctgaa tcatgtgctg gtaatctact gtaagtcaga
     2221 aagttgaaaa cgattgtggc tcactgaaaa acaattagaa tagcggtggg cgcttgaggc
     2281 tgtatgccga aagcgttttg tggacggcat gcagcagaaa gccccgggcg atctttttat
     2341 caatcaaccc aaggcccact gtaatgcctc aacaacatta tagtagcccg atgacggccg
     2401 aaaggcaatg gaggggctgc gatgccacaa cgaacgtttt taacaatgtt aattgtcgtc
     2461 tgtgtgacga tactgtgttt tgtctggatg gtgagggatt cgctttgcgg attccgtgtc
     2521 gagcagggaa acacagtgct tgtggcaacg ttagcctacg aagttaaacg ttaacgggca
     2581 acacggcggc aggaaatctg ccgccggatt gttgcgaaag caaacctcaa tgcgccttgt
     2641 ttgtcttttt taccggacga ctgctggttg ctgctgtccg gcagctatta ttttttccgt
     2701 cctgtgcggc atcaattatg acagtcgctc tcatttggtt gtctctggtt agatgtatta
     2761 ttagatattt tcggggtaat tcgataaaag ttatccactt cagcaccaga ctgaatgata
     2821 tatgcaataa gatctaaacg atgaatctcg tatcgtgtac gtaactccat catttcacgc
     2881 catgctaaaa tccttgatgt tttcagttca gcctctataa gtttccatgt gtttgttgag
     2941 atggcatgtt cttccagctg acgtaacgag aacctatcac taatgaccaa ccatccccat
     3001 cctcgagtat gtgcccattt gcgacctgca ttacttttta ttctcgtgag actcaaggtc
     3061 attcggtctg ttggcttcac ttccataagt aggca
//
LOCUS       DAZGBC010000310         3074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGBC010000310 DAZGBC010000000
VERSION     DAZGBC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.312"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..626
                     /locus_tag="ACHK1J_005045"
     CDS             <1..626
                     /locus_tag="ACHK1J_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914175.1"
                     /translation="AVMFSLIIYIENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEF
                     IEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
                     QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPD
                     FEFVQQKRKEGLSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA"
     gene            623..1009
                     /gene="trbI"
                     /locus_tag="ACHK1J_005046"
     CDS             623..1009
                     /gene="trbI"
                     /locus_tag="ACHK1J_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO7914176.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            1006..1638
                     /gene="traW"
                     /locus_tag="ACHK1J_005047"
     CDS             1006..1638
                     /gene="traW"
                     /locus_tag="ACHK1J_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO7914177.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEADMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            1635..2627
                     /gene="traU"
                     /locus_tag="ACHK1J_005048"
     CDS             1635..2627
                     /gene="traU"
                     /locus_tag="ACHK1J_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO7914178.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAVDAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            2839..>3074
                     /locus_tag="ACHK1J_005049"
     CDS             2839..>3074
                     /locus_tag="ACHK1J_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914179.1"
                     /translation="MKAVEVYGRHSDVVMSLERQTDLLEAAGNVLKQRWFYRPWGSSK
                     ASVMLTGILVIPVFLVLSGLLSAGYPGLLCITVSG"
BASE COUNT          728 a          725 c          919 g          702 t
ORIGIN      
        1 ttgcggtgat gttcagcctt atcatttaca tcgagaaccg gatgtaccgc acgccgcgta
       61 acctcaagaa actgaacgtc attgatgagg ggtggcgtct gctggacttc aaaaatcaca
      121 aggtcggtga gtttattgag aaaggttacc gtaccgcccg ccgtcatact ggtgcctata
      181 tcaccatcac acagaacatc gtcgactttg actctgacaa ggcctccagt gcggcccgtg
      241 cggcatgggg taactcctcc tacaaaatta tcctcaaaca gagtgcgaag gagttcgcga
      301 aatataacca gctgtatccg gaccagttcc tgccactgca gcgcgacatg attggtaagt
      361 ttggtgcggc caaagaccag tggttcagtt ccttcctgct gcaggtggaa aaccactcct
      421 cctggcaccg tctgtttgtg gacccgctaa gccgtgccat gtacagctct gacggcccgg
      481 attttgagtt tgtgcagcag aaacgtaaag aggggctgag tattcatgag gcggtgtggc
      541 agctggcgtg gaagaagtca gggccggaga tggcttcgct ggaagcatgg ctggaagaac
      601 atgagaaata caggagtgtt gcatgaccac aacgcaaaaa acgactgatg tgacagcccg
      661 tcgacgcagc cactggtggt ggacggtgcc gggatgcctg gcaatggtgt tactgaacgc
      721 agccgtcagt tacggcattg tcagactgaa cacgccggta accgtcgcct tcaacatgaa
      781 acagacagtg gatgcgtttt ttgacagcgc cagccagaaa aaactgtcag aagcgcagtc
      841 gaaggcgctg gctgaccgtt ttaacgcggc actggaggcc agtctgcaga cgtggcagca
      901 gaagcaccac gctgtcattc tggtgtcgcc tgccgtggta cagggggcac cggatatcac
      961 ccgtgaaatc cagcaggata ttgcccggcg aatgagggca gaaccatgag atgccggggg
     1021 ctgattgccc tgctggtatg ggggcagagt gtggctgcag ctgatcttgg cacctggggg
     1081 gatttgtggc cggtaaagga ggcggacatg ctgacggtga ttatgcagcg tctgacagct
     1141 ctggagcagt ccggagagat gggccggaaa atggatgcgt ttaaggagcg ggtgatccgc
     1201 aacagcctgc ggccccctgc cgtgcccggt atcggtcgga cggagaaata cagcagccgc
     1261 ctgtttgacc cgtctgtcag actggctgcg gatatccggg ataacgaagg tcgtatcttc
     1321 gcccgccagg gggaggtgat gaacccgctg cagtatgtgc cttttaacca gacactgtat
     1381 ttcatcaacg gtgatgatcc tgagcagatt gaatggatga agcgccagac gcctcccaca
     1441 ctggagagca aaattatcct ggtgcagggc agtatcccgg agatgcagaa ggctctggac
     1501 agtcgtgttt actttgatca gaacggcgtg atctgccagc gtctcggcat tgatcaggtg
     1561 ccggcacggg tgagtgctgt cagtggcgat cgtttcctga aggtggagtt tattccggtc
     1621 ggggagggag ggaaatgaaa cggtgtatgt ggttgtgtgt ggttttgctg ctgactggcc
     1681 atgttcccgc agcctcagcg gacgctgcct gtgaagggcg gtttgttaac ccgatcactg
     1741 atatctgctg gaactgcatt tttccactgt cgctgggcag cacaaaagtg agtcagggga
     1801 aggtgccgga tacaataaac ccgtcaatgc cgatccagtt ctgtcctgcg ccaccgcctg
     1861 tatttaaacg tatcgggctg gctattggtt actgggagcc gatggcactg acggatgtga
     1921 cccgcagtcc gggatgtatg gtaaatcttg gaataacaat cccggagctg gggaaaaccg
     1981 ggcagggaac ggccaaaaaa gacgacaagc aggtgaacgg ggcattttac catgttcact
     2041 ggtacaaata cccgctgaca tactggctga gtattatcac ctcagcagga tgtctgatgg
     2101 gcggcgatat ggatatcggg tacatctcag aaattgaccc tacctggaca gacagcagtc
     2161 tgaccaccat tctcaacccg gaagcggtca tctttgcaaa tccgatagca cagggagcct
     2221 gtgcggtgga tgccattgcc agtgccttta acaagccgct ggatatcctg ttctggtgtg
     2281 ccggttcgca gggcagtatg tatccgttta atggctgggt gagcaatgaa tccagtccgt
     2341 tgcagtcatc cctgctggtc agtgaacgga tggcgtttaa gctccaccgt cagggaatga
     2401 taatgaacag catcggcgtg gatgtggctg tctgttatga atatccgtcg cccatacttc
     2461 ccaaagagcg ctggcgttat cagatggtga atatgtaccc ggacagcgga cagtgccatc
     2521 cgttcggacg cagtgtgatg cgctgggaga cgggaaaaaa tccgcccaac acaaagaaaa
     2581 acttcggtta cctgatgtgg cgtaaacgta actgtgtgtt cctgtaatcg ggaaaatgtg
     2641 atgcgcaaca gtaaagtgca acttgtatca atcctcagac aggtcagtct ttcactgaac
     2701 acggagccat tacgacagtt tatctctctg agggagattg cagaagagac ggatcacgtc
     2761 gctgctcgtt tgtccggagg aaaacgggta acacctgcac aaatttatga attgtgtgca
     2821 cagttatgga tggccaggat gaaggcagtt gaagtttatg ggcgacattc tgatgtggtg
     2881 atgtctctgg aaagacagac tgatttactt gaggctgcag gcaacgtatt aaaacaacgc
     2941 tggttttacc gtccgtgggg atcatcaaaa gcatctgtga tgctgacagg tatactggtg
     3001 atccccgtat ttctggtttt atccggattg ttgagtgcag ggtatcccgg gttattatgt
     3061 attaccgtat ccgg
//
LOCUS       DAZGBC010000311         3014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGBC010000311 DAZGBC010000000
VERSION     DAZGBC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.313"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..726)
                     /locus_tag="ACHK1J_005050"
     CDS             complement(<1..726)
                     /locus_tag="ACHK1J_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7914180.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISA"
     gene            complement(794..2275)
                     /locus_tag="ACHK1J_005051"
     CDS             complement(794..2275)
                     /locus_tag="ACHK1J_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO7914181.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESGIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            complement(2268..>3014)
                     /locus_tag="ACHK1J_005052"
     CDS             complement(2268..>3014)
                     /locus_tag="ACHK1J_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7914182.1"
                     /translation="FRFGPAVARMANLLLQREGETREVKGCGGDDEVLLKCHACEHLQ
                     GHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELEDLYWFQVDMPERMHSD
                     RLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLELLRQYTVTNEQDADIT
                     VSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLYVAVTRALRTLVLNDLM
                     HQLAEEQQKTEGLRGTGYDTSGRVQENGHA"
BASE COUNT          710 a          961 c          608 g          735 t
ORIGIN      
        1 ggcagagatc ggctcactga aacgacagac gtcaaaaaga tagtcatcat cacctgcccc
       61 gcactgacgg gcccgctcca gcagtttttc catcgatacc ttcaggggag ccaccagagg
      121 aacgccccat tccctgtgag ttttacttcc ctccagctta agatcaatta tgctttcatc
      181 cggacagaca tccctcacac ggatatgctt cagttgatta aagcgcattc cggtataacg
      241 cagaacatcc agaactgtca gccagtacca ggcagggtat atagcattgc gtcgacgact
      301 attcaccatg ttctgctctt ccgcttctgc aaacctctgc acaaccaggt aaaccctgag
      361 catctgattg tcactcagtg tctttttctt tttttcagta ctcgcaactg atgcttccac
      421 caacgggttt tctgtctgag gcagcagttt catcttgatc ccgaatccca taattgcccg
      481 caagtgggcc accttgttat tccaggtcac ctccctgcgc ttctgctctt tcaggacata
      541 acgccggtat cgctgaacat cacggacagt cacctccccc ggcaaaatat caccgataaa
      601 ttccctgaat accctgacaa ctttgcagta gctccactct gtatccggcc gaagattctt
      661 tgaaaaaaag tactcttcca gcagttgttc ccaggtgatt tttgtgacgg aatcgtcatt
      721 ctgcattata tattcccctt gtttactgtg ctcccgcact ctccggtcaa attctgcaca
      781 tcatcgcctt tactcacccg aaaacaataa gcgggctgtc tccgggaacc gtaatattcc
      841 cgtacagaaa cctgccctta accagatatc ccgaaaccat tttgtaacgt ccggaatgtt
      901 ctgcgtcact ataaagacgg gcctgaaaaa aacgctctcc tgaacgtacc ttatggatac
      961 ctgcccgttc aaagagcgac tgaacataac gacgatcctg cccggaacca ctctccttca
     1021 gaaaacgata aaaaatcccc ggggcacaca gaaaaacata tccggaaaca acatgaacat
     1081 ttgaatcaac gctgttaacc gaaagtgaac cctccctcag cccctctttt aaccatctga
     1141 taaattcact ctctaccctg ctatcctctg tcagggaaaa ctctgccgtt ttttccactg
     1201 tttccgggat acggtttccc tgattttctg ttacacatcc gctctcttcc ggtaactcaa
     1261 taccgctttc tttatccggc ggcacgcctg acgctatctg ccctccctca tccgacaaat
     1321 cagcagcaga aaacagcgta aggagcattc cggtatcagc atcagtttcg ttcccctcat
     1381 ccacctcctg ttccgctgtc cggccctcat tttcggcatc ctccactggc gactgtacaa
     1441 ccggcaacgg ctgaatgacg ctttctgctg aaattgctgc tgactgcagc agtaccggaa
     1501 caacctcagc caccggaact tcggccacat ctcctgcagc gaggggactc acgacttcct
     1561 ccatagcttc ctgtaatact gtacgtatca gtgccatttc aggatgctga tgccagactg
     1621 ctccggcaag attctgtaat gcccccggga cagtggcaag ccaggctccc gcctctccgg
     1681 gcagcagttg tcccgccatc agggctgcca gttgccgtcc ctccgttccc tggcagggca
     1741 tcttcctgaa ccggaaccgg tatacgccct caggtacaga tatccccggc tgccagcatc
     1801 tgccgtcggc ctgttcgcct tccagttccg ccagcaacgg caggtgataa aacagagccg
     1861 accagaacac caccatgttc cacacactcc cctgcgcggc ctgctcttct gctgtcgctc
     1921 ccggtggaaa cattctgcct ctggccagcc ggacagcata cgtcgtgaac tgcagggtca
     1981 ggtcaccaaa accaccggtg taaccccagc gaccttctgc tgtggccggc agattctgac
     2041 tgacagacaa caactgatgc acaggatcaa gacaaaggcg ctgatacata tccaccggca
     2101 tggaactgtt ttcccataac tgtttcagac accttttcct gaacggtgtg gataccagta
     2161 catcagccgg caatggtgta aaatacccct ccggcaccgt ttccccggga atatccgtcc
     2221 ggacatcaga acattctgtc ttcacaggaa ataacctttt caggcgttca agcatgccca
     2281 ttctcctgaa cccgacctga tgtgtcgtac ccggtccccc gcagaccttc agttttctgc
     2341 tgttcttctg ccagttggtg catcaggtca ttcagtacca gcgtcctgag ggcccgggtg
     2401 acagccacat ataacagaca ggtttcatca tgacgctcgg cctccgtcat cagcggatcc
     2461 gtgatatcct ggaaatcctc atccagcatt acaaccggcc actccagccc cttgctccgg
     2521 tgtgccgttg acaccgtgat atctgcatcc tgctcattcg ttaccgtata ctgccggaga
     2581 agttccagtt ttttcggtaa cggaaaacac aactccagaa gccgaaggga ctgattcatc
     2641 tccacatcct tcgtggattt tgcaatatat ttatattctt caaaattcct gtagtcccgg
     2701 cgcagcctgt ctgaatgcat ccgttccggc atatcaacct ggaaccagta gagatcctca
     2761 agttccccgg tcctgtaacc ttcgattccc cccacccagt aaaccttttg ccctttcatt
     2821 gccgccatca gcgcagtacc aatcacaccc gcgacagtac gactgagtac cgtgtaatgc
     2881 ccctgcagat gttcacacgc atggcatttc agcaatacct catcatcacc accacaacct
     2941 ttcacctctc gtgtttcacc ttcacgctgc agaagcagat ttgccatacg agccaccgcc
     3001 ggaccaaaac ggaa
//
LOCUS       DAZGBC010000312         2969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGBC010000312 DAZGBC010000000
VERSION     DAZGBC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.314"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..215
                     /gene="subB"
                     /locus_tag="ACHK1J_005053"
     CDS             <1..215
                     /gene="subB"
                     /locus_tag="ACHK1J_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO7914183.1"
                     /translation="KNSSVWGPSFSTLYNQALYFYTTGQLVRIYYEPGVWTYPPFVKA
                     LASNALVGLSTCATSTECFGPDRKKN"
     gene            complement(531..1517)
                     /locus_tag="ACHK1J_005054"
     CDS             complement(531..1517)
                     /locus_tag="ACHK1J_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7914184.1"
                     /translation="MYRHDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1617..2351
                     /gene="nanR"
                     /locus_tag="ACHK1J_005055"
     CDS             1617..2351
                     /gene="nanR"
                     /locus_tag="ACHK1J_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO7914185.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(2387..>2969)
                     /locus_tag="ACHK1J_005056"
     CDS             complement(2387..>2969)
                     /locus_tag="ACHK1J_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7914186.1"
                     /translation="SPAEEENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFSAG
                     GHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLGDN
                     STSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYE
                     KGGHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          914 a          670 c          547 g          838 t
ORIGIN      
        1 tgaagaattc atctgtctgg gggccttcgt tttccacatt atataatcag gcactatatt
       61 tttacacaac aggtcagttg gtcaggattt attatgaacc cggggtatgg acgtatccgc
      121 cctttgtgaa ggcattagcg tccaatgccc ttgttgggtt atcgacctgc gccacatcga
      181 ctgaatgttt tggccccgac agaaaaaaga actaataggt gatgtttccg tccagagata
      241 ctctggatgg agtgtcggcg tcagtgatat aagactcgag tgaacactga gccgacctcg
      301 aaatgacgtt gtaggtgcag acatgccata atgggactgt cgatgatatt acagggagtc
      361 acgtcccctg gtgcgtgcct tcgtgacaca ctaccgtcca catagtatca gcaccagtga
      421 tatcaaacgg taacgaccag ctatcctggt gttgataccg acaactccat accactccgt
      481 catgtctctc agagaatcat gtcagagctg tagatatatc ctgaacttgt ctacaaataa
      541 tttcttattt tttgcgcata acattcagca ataactcccc ttctggccat tgaattaaag
      601 tgtgtacttc tctgtgacgt tgtccagctt aattctgtcc tccatgatga ccccagatac
      661 ccgacagata aatcatcggc atcttcttct ggctcattag ttaattcatg tcggttacta
      721 tcctgaaagc tgagaaaatg tatttcatca tcagtccgtt gtctgtaatg tccatatata
      781 aaatcatatt ctttttgata agattccttc cagtaccagg ttgtatcacc acacaaccat
      841 ggaactcttt ctgacgaatt accaacacac tgagatgagt attcagataa ctcagacctg
      901 aaagaagtta ccatctgata aaacaagtca ggatgctttt cataatctgg tgacataaga
      961 tcaaactccc cctgcatcca gcagacagat agcagtttat tctttctgtt atattccaga
     1021 gctaccttaa cccgggctac taagtcctca tagagtgctg ttccggtgcc ccaccgcatt
     1081 gcatcagctg ttgcccctgt atcaggtaca tacatacctt cagctccaac agtaaaggcg
     1141 gcacctcctc tgcaacaagg aaccagtaat atccctgctt gctcaggtat gtatggcagt
     1201 aactttttag ctatatgcaa tccctgtccg acacaaccat attccccttt atgcaaatca
     1261 gcagccggat ggtgatatcc actcatatcc tgaacatcat gcaggcagtg atctgccgga
     1321 ataatctcat tgaatttaca ctcttttcct cctggtgtga ttgtttttcg acgagccagt
     1381 tgttttacac gggactcggg tttgtcgagt gtatctggca ggggaatacc ttcaccgtag
     1441 gccataccat tagactgtcc tgcgagagct atcacatacc agaaatctat tttttcatta
     1501 aaatcatgac gatacatcac tcccccttta cataatagat ttaattaaat attttcatta
     1561 ttaaaagaaa gtgttaaatt aaaaactcat ttgctctaat ccatataaat caatatatgt
     1621 ccgacaacaa aaaaaccgtg ttacccgggc taacaacgac cagaaagccc attgttacaa
     1681 aaaaactttc agaaatcgtt gccgaagagc ttgaaagaat gatccgccag gaggagataa
     1741 gggaaggtga atacctgcct tctgaacgtg aacttatggc attctttggc gttggaagac
     1801 catctgtcag agaggcttta tcatctctca gacataaagg gctaatccgg tataacaagg
     1861 gagaaaaagc cagtgtctgc cgtccatcac cagaaaccat cattaatggg ttatcaggca
     1921 ttgttaaaga ttttttatcc acagctgatg gcatcaagga ttttgaaaga ctacgcatct
     1981 tttttgaaac caaccttgta agatatgcag caaaaaatgc aacgcctgag gatattcaca
     2041 ggcttgagaa ggtgctcagt atccatcata actcactgag aacccccgaa gtgtatgctc
     2101 aatatgatat tgaatttcat agtatacttg ctgatatacc tgacaataaa gtattaaaaa
     2161 caatacactc agcattttct gaatggctga taatggccaa acctctggaa aagaataaaa
     2221 tgacatatga aaataacaaa aaaacccatc agcagcatat tgaaatactt acggccataa
     2281 aagagcatga ttgtgataaa gctgaacagc taatgtataa tcacctttat aaacgaattg
     2341 aagcatcata gttactcgac gcttcaccag gtagtcacca taaatattat ttaaatccat
     2401 gagaatacat ccagtccata aacagtttta tccatcctcc aacaggaaga tctgtggccc
     2461 actgaacact aaagccatgc cctcctttct catagagatg catttcagca gaaacgcctt
     2521 tctctattag gccacggtac atgagcagac tgttttcaat tgacacaaga tcatcatcac
     2581 aacaatggag taaaaacaca ggaggaaaac atgatgtgac attaagatcc gcactatatt
     2641 gtattatatt ttctgaggta ctattgtccc ccagtaattg cttcctggag cctggatgtg
     2701 tcacatcatc tttcattgat attacaggat aaattaaccc gacaaaatcg ggaatagcga
     2761 aaatctcttg ttctttactg tcagaagaac gtttgaaatc acaaatctga ttcacaaacg
     2821 ttgaggcgat atgcccccca gcagaaaatc ctaatacacc aatacgatga cgctgtagat
     2881 gccatttatc agcattctta cgaatataaa gcattgcaga tattgcatca ataaaagttt
     2941 tgtttatatt ttcttcctca gctggagat
//
LOCUS       DAZGBC010000313         2945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGBC010000313 DAZGBC010000000
VERSION     DAZGBC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.315"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            209..1975
                     /locus_tag="ACHK1J_005057"
                     /pseudo
     CDS             209..1975
                     /locus_tag="ACHK1J_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1975..2352
                     /locus_tag="ACHK1J_005058"
     CDS             1975..2352
                     /locus_tag="ACHK1J_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914187.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DVSKFLGDKSKSHALVWKNFGQE"
     gene            2821..>2945
                     /locus_tag="ACHK1J_005059"
     CDS             2821..>2945
                     /locus_tag="ACHK1J_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO7914188.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGA"
BASE COUNT          832 a          571 c          848 g          694 t
ORIGIN      
        1 taagcctggg tgtaatgcaa tcaaaagatt gtaacgaatg aaataatctg taagtgaggt
       61 ggcgttaatt aggacgataa tcgcatgaaa aatcaccccg ttaaaatttt ttatgaatca
      121 ttaactagta ttgttgctcc ttgtatgaca aaaccacagc atgtgcagga aatatgcaca
      181 tgttgtggac gccctgcaag ggaatttccc gtatgcaaca gacccggcgg gaaaccggct
      241 gccggacccg gaactgcatc cggacagcac gctcacggcg tggccggata accgcatcgc
      301 ggaagatgcg cactatgtct accgctacga tgaatacggc aggctggcgg agaagacgga
      361 ccgtatcccg gaaggggtta tccggatgca cgacgagcgc acccaccact atcactacga
      421 cagccagcac cgcctggtgt tctacacgcg gatacagcac ggcgaaccac aggtggagag
      481 ccggtacctc tatgacccgc tgggccgccg gacgggaaaa cgggtgtggc ggcgggagcg
      541 tgacctgacg gggtggatgt cgctgtcgcg taaaccggag gagacgtggt acgggtggga
      601 cggcgacagg ctgacgacgg tacagaccga caccacgcgt atccagacgg tataccagcc
      661 ggggagcttc acaccactca tcagaatcga aacagagaat tgcgaacagg cgaaggcgcg
      721 gcaccgtagc ctggcggagg tgttgcagga ggacacgggg gtgacgctac cggcggagct
      781 ggcggtgatg ctgggaaggc tggagcggga actgcgcgcg ggagcggtga gtgcggagag
      841 tgaggcatgg cttgcgcagt gcgggctgac ggcggagcag atggccgcac agctggaggc
      901 ggaatacatc ccggagagga aacttcatct ttaccactgc gaccaccggg gactgccgct
      961 ggcgctcatc agcccggaag gggaaacggc gtggcagggg gagtatgacg agtggggaaa
     1021 cctgctgggc gaaaccagcg cgcagcacct tcaacagtca ctccgtctgc cggggcagca
     1081 gtatgatgag gagtcggggc tgtactataa ccggaaccgg tactacgatc cgttgcaggg
     1141 acgctatatc acccaggatc ccattgggct gaagggggga tggaacctgt atgcgtatcc
     1201 gctgaacccg gtgcagcggg gggatcctct tgggttgtct ggtgagacac tggttcttgg
     1261 ttctgccaca acagtaacag gaacagaatt tgggggggcg ttactttcgg gtggggcggc
     1321 gacttttgat gcagtcacgg cggctatggc gtctgctgcc cctgctcttg gagcaatggc
     1381 tgtggtcgct gggccaatgg gatatgacct tctcgctaat gatgggcgtg ccgggaaggc
     1441 tctgagcgat gggcagttaa taaattctca ggctgaaatg tgttactgga ccagtagttg
     1501 tcctttagat agcccacatg agaatgataa ttcgtcaggg agtcagccga atgttgggaa
     1561 ggatttgacg gatgaagaga agaactgcct ggccggcggc agtacgggaa gcccgggagg
     1621 atggggacca gaagatgaga atagcgcaag ggatcgtatg cagcatgaac ggtataaaga
     1681 tgaattgcga cagaaaatgg gaaaacccga cgttacagat tcaaatttga aaaatattat
     1741 agacgatctt tatcggccaa atgcaaaagt aggaagtgga agcacagcag atgctgttcg
     1801 ttatgaattg gccacaggtg aaaaagttgg tggtagagga catgtattga aagcacagga
     1861 ttatacaagg gctttacagg attggttgga taagaatcca acagcatctc caggggatcg
     1921 agcagcagca gaaaacgttc ttcaggattt acagaatgct ttgggaggca agtaatgaac
     1981 caatatattg ctagttatat agaaaaaatg gcatcatttt ccactgtttt gtctgagaca
     2041 gtctcattag ctaatgaata ctggcatcca gaaacgccac cggtggttat gttgttctct
     2101 caggttggaa aaacgattgt aaaaaatttt tatgaaatgg gggatattga aagagaaaaa
     2161 atatttaaac atgtcgaaga tggtatggtc tcgcttgatg atgaattggc cacagttgta
     2221 gctacagggc ttatagaggc aatagtcact gcgaccgata gcaattcttt actttggcaa
     2281 gatgtaagta aatttctggg tgacaaatca aagtctcatg cattagtgtg gaaaaatttt
     2341 ggtcaggagt gatattattc aagaagtatt aagctcgcta ttgagatctt aatgcttctt
     2401 gagattaaat taaagttagg acgaagtaat tgcaggtgga aataaatcca tggtgacgtg
     2461 tggcaatggc tattagatgt tatggtgtga tgggttttgg tgatttaaga gctaagttag
     2521 attgccttat gaaaaagtat ggttatatat ttaatgatgt gtatcatata tttaaataaa
     2581 aaacggttct ctcttgtcaa gatagtaata ggatagatta ttgtgttgat aaatataatt
     2641 aagagtctac attgttgatt ttagaagact ttggagcagg gaaaggtcat cagaagtaca
     2701 ctttttgtac taaataattc gcgctaaatg ttcaataatt gagatattcc ttattaccta
     2761 aaactatttt tcatagctta tatatgatca aatactcctt gcataaataa ggagaacaaa
     2821 atggaactta aaaaactgat ggaacatatt tctattatcc ccgattacag acaagcctgg
     2881 aaagtagaac ataaattgtc agatattcta ctgttgacta tttgtgccgt catttctggt
     2941 gcaga
//
LOCUS       DAZGBC010000314         2923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGBC010000314 DAZGBC010000000
VERSION     DAZGBC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.316"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2923
                     /gene="ypjA"
                     /locus_tag="ACHK1J_005060"
     CDS             <1..>2923
                     /gene="ypjA"
                     /locus_tag="ACHK1J_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO7914189.1"
                     /translation="DATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNINSGT
                     QNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETGSAL
                     VANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGKLIV
                     QKEAKTDGTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDAFYI
                     RNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGSVLNSAGTQTIYASA
                     TSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAINTDI
                     VSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQSSQ
                     GALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNTGGR
                     EIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTLTVN
                     DNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLVLAG
                     TEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGTAIV
                     YAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLTA
                     ASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTTNEL
                     SGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDAS
                     FSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDPKPD
                     PKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNN
                     NVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGF
                     DR"
BASE COUNT          838 a          688 c          797 g          600 t
ORIGIN      
        1 ctgatgcgac ccgaatagat aatggcgggg ttatggatgt tgcaggaaac gcgacaaata
       61 ccataattaa tggtggcaca cagaatatta ataattatgg catagccaca ggcaccaata
      121 tcaacagcgg aacgcaaaat atcaaaagcg gcgggaaagc tgacacaaca attatatcct
      181 ccgggagccg gcaggttgtt gagaaagatg gtacggcaat tggcagcaat attagcgccg
      241 gaggctcgct gattgtctat accggcggta ttgcacatgg ggttaaccag gagacgggca
      301 gtgctttagt tgccaacacg ggtgcaggga ctgatatcga aggatacaac aagctctctc
      361 acttcactat taccggaggg gaggctaatt atgttgtgct ggaaaatacc ggcgaactga
      421 cggtagtggc taaaacctcg gcgaaaaata ctaccattga tgctggcggt aagctgattg
      481 tccagaagga ggctaaaaca gatggcacca gacttaataa tggcggcgtt ctggaggttc
      541 aggacggtgg tgaggctaag catgttgagc aacaatccgg cggcgcatta attgcttcca
      601 cgacctccgg aacacttatc gaaggaacca acagttatgg tgatgctttc tacatcagga
      661 attcagaagc taaaaatgta gtgctggaaa acgctggctc attaacagtc gtcactggtt
      721 cccgggcagt tgacacgatt attaatgcca acggcaaaat ggatgtttat ggaaaagatg
      781 ttggctctgt actcaatagt gctggcaccc aaacaatata tgccagtgcc acttctgata
      841 aagcaaatat caaaggtggc aagcaaacgg tatatggttt agccactgaa gcaaatatcg
      901 aaagtggtga acaaattgtt gatggtgggt caacagagaa aacacacatc aatggtggca
      961 cgcaaaccgt tcagaattat ggtaaggcga tcaataccga tatcgtctct ggcctacaac
     1021 aaattatggc aaacgggaca gcggaaggtt ccattattaa tggcggttca caggtagtta
     1081 atgagggcgg tctggctgaa aactcggtgc ttaatgacgg cggcacactc gatgtgcggg
     1141 agaaaggcag cgcaacgggg atacagcaga gtagccaggg cgcgttggtt gcaaccacca
     1201 gggcgacgcg ggtcacagga acacgcgcgg atggcgtcgc gttcagcatc gagcagggtg
     1261 cggcgaacaa tatcctgctg gcaaatggcg gcgtgttaac cgtggagtca gacacctctt
     1321 ctgacaaaac acaggtcaat acgggcggac gggagatcgt caaaacaaaa gccactgcga
     1381 caggcacgac gctcaccggc ggtgaacaaa ttgtcgaggg tgtggcgaat gagacaacaa
     1441 ttaacgacgg cggaatacaa acagtttcag ctaacggaga ggcaataaaa acaaagatca
     1501 atgaaggcgg tacgctgaca gtcaacgata atggcaaagc gacagatatc gtccagaaca
     1561 gcggtgccgc tctccagacg agcacggcta acggtattga aatcagcggt actcaccagt
     1621 acggcacttt ttccatttcc ggcaatttag cgaccaatat gttgctggaa aatggcggta
     1681 atttattggt attagcaggt accgaagctc gcgactccac ggttggcaag ggtggggcaa
     1741 tgcaaaacct gggtcaggac tccgccacaa aggttaactc tggcgggcaa tatacccttg
     1801 ggcggtcaaa agatgagttt caggctctgg cccgggcaga agatctccag gtcgctggcg
     1861 ggacggcaat cgtctacgca ggtacgctgg cggatgcatc ggtcagtggc gcgacaggaa
     1921 gcctgtcgtt aatgacgcca cgggataatg ttacgccagt taaactcgaa ggggcggtcc
     1981 ggattaccga tagcgcgaca ttaactctgg gaaatggcgt cgatacaacg cttgccgacc
     2041 tgacggctgc cagccggggc agtgtctggc ttaacagcaa taattcctgt gcaggtacca
     2101 gcaactgcga atatagagta aacagtttgc tactcaacga cggtgatgtt tatttatcag
     2161 cacaaacagc agcgcctgcc acaactaacg gtatctacaa tacgctgaca accaatgaac
     2221 tttccggtag cggtaatttc tacctgcata ccaacgttgc aggctcccgg ggcgatcaac
     2281 tggtcgtcaa caacaacgcc actggtaatt ttaaaatctt tgttcaggat accggcgtca
     2341 gcccacagtc tgacgacgcg atgacgctgg tgaaaacagg gggaggggat gcttcgtttt
     2401 cgctgggcaa tactggcggt ttcgttgatc ttgggaccta tgagtatgtc ctgaaaagcg
     2461 atggcaacag caactggaac ctgaccaatg atgtcaaacc caacccggat cccaacccaa
     2521 atcccaaccc aaatccgaag ccggatccaa aaccagaccc aaaaccggat ccgaaaccag
     2581 acccgactcc cgatccaacg ccgacacccg ttccggagaa acgcatcacg ccttctacgg
     2641 cagccgtact caatatggct gcaacattac cgttggtatt tgatgctgag ctaaacagta
     2701 ttcgcgagcg gttgaacata atgaaagcga gtccacacaa caataatgtc tggggggcga
     2761 cgtataacac ccgtaataat gtcaccaccg atgcgggtgc cgggtttgag cagacgctga
     2821 ccggaatgac agtggggatc gacagccgta atgatattcc tgaaggaatt gccacgctgg
     2881 gcgcttttat gggttattcc cattcacata tcggttttga tcg
//
LOCUS       DAZGBC010000315         2900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGBC010000315 DAZGBC010000000
VERSION     DAZGBC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.317"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(51..1406)
                     /locus_tag="ACHK1J_005061"
     CDS             complement(51..1406)
                     /locus_tag="ACHK1J_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO7914190.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMVSAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            complement(1744..>2900)
                     /locus_tag="ACHK1J_005062"
     CDS             complement(1744..>2900)
                     /locus_tag="ACHK1J_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7914191.1"
                     /translation="WVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVF
                     SLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSL
                     SNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAY
                     AGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTA
                     VAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLW
                     RQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPI
                     LIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT          749 a          756 c          692 g          703 t
ORIGIN      
        1 taagacaaag gtaatcactt aacaacggcc cactagtaat aatggcagga ttatttcaag
       61 tcgggtaagt cgttgtataa gccctgttta attcccgcta atggaataag gttattcacc
      121 gcagcggcat aatttacggc ggcgacccag ccatcataat tggcatttat ttcagccgat
      181 tgcgcctgaa agacatcttg ctcgacggtt aatagatcat tcagactgcg tttacccaat
      241 ttatattcat tttggtacac atctcgcgtt ttgtgcgcac tttcggattg cgctaaaccg
      301 gcttcttcac gaccccgtgc gccggtccag ttcgcatatg ccacagacgc tcgttgcagc
      361 acatccagtt ttgcctgttc gacctgcgag gcagagattt tttgttgccc ttcggcctgt
      421 tgcacctggg cagaaaccgc gccgccctga tacagcggcg cgttaacgtt cagttgtagc
      481 tgatcatccc aatacgagcg gtcgctggtc tggtagcgcg ttttaccccc ctgaatactg
      541 agcgttggcc agtattgcgc tttcgttttt tccacgccgt actgtgctga ctggcgtaag
      601 ttttctgccg ccagcaccag cgggatggac tggtaatcaa tattttttag cgataccggc
      661 tgctcggcta attcagccgg tggcgcggct atcgcctccg gctgtacgcc agtgagcacc
      721 gccagttgcg ctttggcgct caccatctgc gcctgatatt gctccagcgt tgagcgcatt
      781 ccggcaatac gcgtctgggc ctgtaattca tccgacgatg agttcaggcc agcgttagcg
      841 cgcaatgccg ccatgttgta gacgttttcc agcgagtgaa tattgcgttg agccgcatcg
      901 cacaaagcct gataacgact gacttccata taggcagtcg ccgttttctc tgcaacatcg
      961 gttaaggttg ccatcaattt aaagcggtag ctgtcacggg cggcagtttg cagattgata
     1021 tcgttgttag ttttaccaaa gtcatacacc agctgtgtca gggttatgcc atacgaaacg
     1081 ttgttatcaa gcctgccgct ggagtccgtt tgtcgggacg gccccgcgtt gccggttaat
     1141 ccaacgtgcg gataccaggc acttttggct tcatcaattt gcgcttcgcc aatgcctatc
     1201 tgcgccgcct gttgagtgac gtcgggattt cgcgcaaagg cgcgtaatat actttcctgt
     1261 aaggataatt gggcaactgg cgttaccgct ggggcgttcg cccattgtgg cagcggtgca
     1321 gcaattgtag cttctcccat caccataaaa cttattaaaa aaataaaccc attattttta
     1381 cggcttttaa actggaatcg cgccatagat aagtcacttc cttccaggtc attattttaa
     1441 ctggttttta taaccagtct cattttatta agaagggtaa taacttgtgc atctcttttg
     1501 tcgatcattg atattggcaa agaaatgcag ctaaaaagct tatacgttat ttatttagaa
     1561 tacactacga cttttagatt accatcatac acattaagaa taatccacgg taataagtga
     1621 ttagcatcac agatttatcg cgtatatatc atttttttgt gacttattta ttaaaggaat
     1681 aagaaaggta atttgaatta aagacaaagg cgcagtacag gcagtactgc gcgcagagag
     1741 gtgttatacg gttttattgc gcttcatgac cattgccaca ataaggctga gtaagattgc
     1801 accaataatc catatcaacg cacggctgcc ctgggaccac aggctgtaga taaagccaac
     1861 gatcaccaac aacatcacca acgtacctac gataagaatc ggcaacgagg cgtgaatatc
     1921 atgacgcagg cggattgcca ccacaaacac cgccagatag caaatcaaaa aggtggcgct
     1981 ggcaacgctg gcgagtgagc ctaaatttag tgccgccgtc atcagcataa tcaacaccac
     2041 gacaatgatg ttgccccagg tactctgccg ccacagggat ttattcatta gcttcggcag
     2101 ttcgcgttcg ctgcccatgt tgtccatgat gttaaacacg gcgaacaggt tcgcgttgat
     2161 ggctgaagcc gtcgccagta aagcgccgat gacgacgatc acataaccca catgcccgag
     2221 cagcggagaa gctgcctgcg ctacggcggt atcggcatat ttttctaact ctaatgccga
     2281 tacatcgcta agcaaaacca gcgccagcga gatataaagc aacgtggtaa cgccaattgc
     2341 caccagaaac gcccgtggca taatgacctg cggatctttc actttatccg ccgcgttcgc
     2401 catcatgcca aagcccgcat aggcaaggaa ggttatcccg atacaggaga agaacgcacc
     2461 ggagctgggg ggcgcagaga cggaaatatg cgccggttgc agcgaccaga caccggcaat
     2521 aatcagcaat aacaggatca tcattttaat gccgacgagg atcacctcca gccgccctac
     2581 cgcatggttg cttaaggagt tgaaaagcgt catcaccgca atgatcccca acgcgtagag
     2641 caaaataagg tgctcctcct ggctgccttc atgcaaaaac tgcacggcat aagcgccaaa
     2701 agcacgggcg accatggcga tgctcaccgc cagcgtcaac aggtacagta acgagagcgc
     2761 cagcgaaaag acgccgttgc ctaatccgcg acgaaagaag tcgataatgc cgccattgct
     2821 gggatagctc gcccccagac gcgcataggc ataaccggaa aacatcgcca caataccgcc
     2881 aaaagcaaag gcgacccagg
//
LOCUS       DAZGBC010000316         2881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGBC010000316 DAZGBC010000000
VERSION     DAZGBC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.318"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..183
                     /locus_tag="ACHK1J_005063"
     CDS             <1..183
                     /locus_tag="ACHK1J_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HFO7914192.1"
                     /translation="WYKVIAMPEKQIIRYDEKYLPLENGMKAESTLFLEKRRIYQWML
                     SPFYDMKHSATGPLND"
     gene            176..2272
                     /locus_tag="ACHK1J_005064"
     CDS             176..2272
                     /locus_tag="ACHK1J_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 export transporter
                     peptidase/ATP-binding subunit MchF"
                     /protein_id="HFO7914193.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFQAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKLGDIQSR
                     FDSLDTLRATFTTSVIGLIMDSIMVVGVFVMMLLYGGYLTWIVLCFTTIYIFIRLVTY
                     GNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAYWLNMKIDAINSGIKL
                     TRMDLLFGGINTFVTACDQVVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSERVTSLT
                     SFLLQLKIMSLHNERIADIALHEKEEKKHEIEIVAHMGPISLETNDLSYRYDSQSAPI
                     FSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGRVLINGIDIRQMGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEEMDEEWMVECARASHIHDVIMNMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSALDSESEHFVNVAIKNMNITRVII
                     AHRETTLKTVDRVISI"
     gene            complement(2459..2776)
                     /locus_tag="ACHK1J_005065"
     CDS             complement(2459..2776)
                     /locus_tag="ACHK1J_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="HFO7914194.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVIGGAFGAGLC
                     GPVCAGAFAVGSSAAVAALYDAAGNSNSAKQKPEGLPPEAWNYAEGRMCNWSPNNLSD
                     VCL"
BASE COUNT          824 a          565 c          678 g          814 t
ORIGIN      
        1 tggtataaag tcattgcgat gcctgaaaag cagattatca gatatgacga aaaatacctc
       61 cctctggaaa atggaatgaa agccgaaagt acactatttc tggaaaaaag gcgtatttac
      121 cagtggatgc tttctccttt ctatgacatg aaacacagtg caacaggacc gctcaatgac
      181 taacgggaat ttcagacaaa ttataaatca gcttgatatg cgctggcgac gtcgtgttcc
      241 ggttattcat cagacggaga ccgctgaatg tggactggcc tgcctggcaa tgatatgcgg
      301 tcattttggt aagaatattg acctgatatc tcttcgccgg aagtttaatc tctcggcccg
      361 tggagcaaac cttgcaggaa tcaatggaat agcagagcag ctggggatgg tcacccgggc
      421 tctttcactg gagctggatg aacttggtgc cctcaaaatg ccgtgtattc tccactggga
      481 tttcagtcac ttcgtcgtgc tggtcagcgt aaagcgtaac cgttatgtac tgcatgatcc
      541 ggccagaggc agaagatatc tcggtcggga ggaaatgagc cggtatttta cgggtattgc
      601 acttgaggtc tggcccggaa gtgaattcca ggcggaaacc cagcagaccc gcataagtct
      661 ccgttcactg attaacagta tttacggtat taaaagaaca ctggcgaaaa ttttctgtct
      721 gtcagttgta attgaagcaa tcaatctggt aatgccggtg ggaactcagc tggttatgga
      781 tcatgcgatt ccggcggggg acagagggct gctaacgctt atttctgctg gcctgatgtt
      841 ctttatattg ctcagggccg cggtgagtat gctgcgtgca tggtcctcac tggttatgag
      901 cacgctcatc aatgtacagt ggcagtcggg gctgttcgat catcttctca gactaccgct
      961 ggcgtttttt gaacgccgaa aattaggtga tatccagtca cgttttgact cccttgacac
     1021 attgagggcc acatttacca ccagtgtgat cgggttaata atggacagca ttatggttgt
     1081 cggtgttttt gtgatgatgc tgttatacgg aggatatctc acctggatag ttctctgctt
     1141 taccacaatt tacattttta ttcgtctggt gacatacggc aattaccgac agatatcaga
     1201 agaatgtctt gtcagggagg cccgtgccgc ctcctatttt atggaaacat tatatggtat
     1261 tgccacggta aaaatccagg ggatggtcgg aattcgggga gcatactggc ttaatatgaa
     1321 aatagatgcg ataaattcgg gtattaagct aaccaggatg gatttgctct tcggaggaat
     1381 aaataccttt gttaccgcct gtgatcaggt tgtaatttta tggctgggag ctggccttgt
     1441 gatcgataat cagatgacaa taggaatgtt tgtagcgttt agttcttttc gtgggcagtt
     1501 ttcggaaaga gttacctctc tgaccagttt tcttcttcag ctaaaaataa tgagtttaca
     1561 caatgagcgc attgcagata ttgcattaca tgaaaaggag gaaaagaaac atgaaattga
     1621 aatcgttgct catatggggc caatatccct ggaaaccaat gatttaagct accggtatga
     1681 cagtcagtca gctccgatat tcagtgctct gagtttatca gtagctccgg gggaaagtgt
     1741 ggctataact ggtgcttccg gtgcgggaaa aaccacatta atgaaagtac tatgtggact
     1801 atttgaacct gatagcggga gggtactgat aaatggtata gatatacgcc aaatgggaat
     1861 aaataattat caccggatga tagcctgtgt tatgcaggat gaccggctat tttcaggctc
     1921 aattcgtgaa aatatctgtg gttttgcaga ggaaatggat gaagagtgga tggtagaatg
     1981 tgccagagca agtcatattc atgatgttat aatgaatatg ccaatgggat atgaaacatt
     2041 aataggtgaa cttggggaag gtctttctgg cggtcaaaaa cagcgtatat ttattgcacg
     2101 agccttatac cggaaaccag gaatattatt tatggatgag gcaaccagtg ctcttgattc
     2161 agagagtgaa catttcgtga atgttgccat aaaaaacatg aatataacca gggtaattat
     2221 tgcacacaga gaaacaacgt tgaaaactgt tgacagagtt atttccattt aaaacctact
     2281 gacataacca tagaggaatt gcaactgtat gaggaatata tcttcctgtg ataattcctc
     2341 gttatgttca gttgtcagtt ggaggaggaa tggaagatag acaacccacc aggaagcata
     2401 tgtatcattc tgtgtggatt gtccaataac agttctttcc tatattaaaa aatactattt
     2461 ataaacaaac atcactaaga ttatttggac tccaattaca cattctacct tcagcgtagt
     2521 tccatgcttc tggaggtagt ccttctggtt tttgtttcgc tgagttggaa tttcctgctg
     2581 catcatacaa agcagcaaca gcggcagaag atccaacagc gaaggcacca gcacaaacag
     2641 ggccgcacag accagctccg aatgcaccgc caatcactcc tgcaccgttt gttgctacag
     2701 ttttcccgac atcaacccaa ttgacatcac cagcaccaga gacattattc atttcattta
     2761 acgttatttc tctcatatat catttacccc tattaaaaaa aatagatatc attaatgcaa
     2821 tggcgatacc gctaacaaca gattgaacaa ggttgctaga atttagttgt ttagcaataa
     2881 g
//
LOCUS       DAZGBC010000317         2866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGBC010000317 DAZGBC010000000
VERSION     DAZGBC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.319"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1869
                     /locus_tag="ACHK1J_005066"
     CDS             <1..1869
                     /locus_tag="ACHK1J_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7914195.1"
                     /translation="PESLRPVTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAP
                     VLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYKQYLERDAALE
                     RRFQMVRVDEPDDETACLMLRSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPD
                     KAVDLLDTAAARVRMSLDTVPEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSA
                     IEQEENVLMTARDDLEQQYARECELTGELLESRSDISRQSETLHLQQALHDIQQNQPL
                     LSVDVDVRTVAGVVADWTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRL
                     RAAKTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHT
                     VSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMR
                     DGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLA
                     RFQTVIYRPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERLYDALTSACLLPDTG
                     ARNVESLLNQQLLPVLSRQLLSHMAAKQKPQALTLTWSDEDGIVMVFDAQAEGEPS"
     gene            1866..>2866
                     /locus_tag="ACHK1J_005067"
     CDS             1866..>2866
                     /locus_tag="ACHK1J_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7914196.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYEFGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTTGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLETDG
                     "
BASE COUNT          625 a          764 c          864 g          613 t
ORIGIN      
        1 ccggaatcac tcagacccgt caccctgcgc acccttgacc tcggcctgct gcaggccggt
       61 gccggcgtga agggtgaatt tgaacagcgc ctgaaaaacg tgattgatgc cgtgcagcag
      121 tcaccggctc ctgtactgtt gtttatagat gaagcccata cccttatcgg ggccggtaat
      181 caggccggcg gcgcggatgc ggccaacctg ctgaagcctg cgctggcgcg gggtgaactg
      241 cgtaccattg ccgccaccac ctggtctgag tacaaacaat acctggaacg tgacgcggct
      301 ctggagcggc gttttcagat ggtcagagtg gacgagccgg atgatgagac ggcctgtctg
      361 atgctgcgct ccctgaaatc ccgttatgcg gaacatcata acgtgcatat caccgatgag
      421 gcggtacgtg ctgccgtcac actgtcgcgc cgttatctga cagaacgtca gttaccggac
      481 aaggccgttg acctgctcga tactgctgct gcccgtgtgc gtatgagcct cgacacggtg
      541 ccggaacagc tgacccgtat ccgttcacag ctcgcttccc tcggtatgga aaaacaggca
      601 ctgctggaag atattgccgt tggtcatcag aatcacggcg aacgtctgtc tgccattgag
      661 caggaagaga atgtgctgat gacagcacgt gatgacctgg agcagcagta cgcccgggag
      721 tgcgaactca ccggtgagtt acttgaaagt cgcagcgata tttcccgtca gagtgagaca
      781 ctgcatctgc agcaggcact gcacgacatt cagcagaatc agcccctgct cagtgtggat
      841 gttgacgtac gcaccgttgc cggtgtggtc gcggactgga ccggggtgcc gttatcttcc
      901 ctgatgaagg acgaacagac ggaactgctg catctggaaa aggatatcgg cagacgggtg
      961 gtcggacagg acgtggcact ggaatccatt gcgcagcgtc tgcgtgcggc gaaaacgggt
     1021 ctgacatccg gtaacggccc ccagggggtg ttcctgctgg ttgggcccag tggtgtggga
     1081 aaaaccgaaa cggcactggc gctggcagac gtgatgtacg gcggcgaaaa atcactgatt
     1141 accatcaatc tgtcggaata ccaggagccc catacggttt cccagcttaa gggttcaccg
     1201 cccgggtacg tgggatacgg tcagggcggt attctgacgg aagctgtgcg taaacgccct
     1261 tacagcgtgg tgctgctgga tgaagtggaa aaggcccacc gggatgtgct gaatctgttc
     1321 taccaggtgt ttgaccgggg ctttatgcgc gacggcgagg gacgtgaaat tgacttccgt
     1381 aatacggtca ttctgatgac ctccaacctg ggtagtgacc ttctgatgca gcaactgagc
     1441 gaaaagccgg agacaacgga atcggagctg catgagctta tccgtccact gctgcgcgac
     1501 cacttccagc cagcactgct tgcccgtttc cagaccgtga tttaccgtcc gctgacgcag
     1561 tctgccatgc gcaccattgt ggagatgaag cttgcacagg tctgcgaacg cctgcactgc
     1621 cattatgggc tgagcacatc ggttgatgaa cgtctgtacg atgccctgac atcagcctgc
     1681 ctgctgccgg atacgggcgc ccggaatgtg gagagtctgc tgaaccagca actgctgccg
     1741 gtactgagcc ggcagttgct gagccatatg gccgcgaagc agaaaccaca ggcgctgact
     1801 ctgacatgga gtgacgagga cggtattgtg atggtatttg atgcacaggc agagggagag
     1861 ccgtcatgaa ccgttccctg accagcaagg ctggcagcgt tgtacagcag ctttcagggc
     1921 aatcacggta ccgggttcat gtgcatgaat gcagccattt tctggatgtg ttgcgttaca
     1981 gcgcggttga gtccctcagc cagccctggc gctatgacgt ggttgtcacc tgctcttctg
     2041 cggatattgc ctgtgacgcg ttactgctga agccggcgtc atttactttc caggtcccga
     2101 tgtctgacgg aacaccggcc ctgccgatca ggacggtatt cggcgtggtt gagtcgtttc
     2161 gtcgtatctt tgcctcgaat gatgagaccc gttacgcact gacgattgtt ccccgtatcg
     2221 cccttctgaa ctatacgaaa gggagcgaaa tctacctcaa tcagtcagtc actgaagtgg
     2281 ttgagcaggt attacgtaaa catggtctgg aagggcagga ttttgaattc cggctctccc
     2341 gggagtatcc acctagggaa ctcatcaccc ggtggcggga aactgacctt gagtttatcc
     2401 ggcgcctgct ggcggaggtg gggatttact ggcgctatga aatggacagc cgtcttgaac
     2461 aggacatagt gattttccag gacagccagc agcagtatga atttggcatc acgctgccat
     2521 tgcgcaatca ggccgggatg agtgacagcg ggcaggagag catctgggat attcagacca
     2581 cctgtaacgt ggtgagcgga agtgtggtca cccgggacta caactaccgt gaagcgctga
     2641 aacctcagga cagtgccgaa agtattttca gtaaggaagg gatcaccacc ggagaaacgt
     2701 accattatgc tgaacccttc ctgacgacag gtgacgctga aagcccggag accggggcat
     2761 actttgcccg tctgcgtcac gaacgcattc tgaacgcgca gtattgtgtc agtgggcatt
     2821 cctccagccc ttatctgacg ccgggtcagg tacttgaaac ggacgg
//
LOCUS       DAZGBC010000318         2866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGBC010000318 DAZGBC010000000
VERSION     DAZGBC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.320"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1881
                     /locus_tag="ACHK1J_005068"
     CDS             <1..1881
                     /locus_tag="ACHK1J_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7914197.1"
                     /translation="PESLRPVTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAP
                     VLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYRQYLERDAALE
                     RRFQMVRVDEPDDETACLMLRSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPD
                     KAVDLLDTAAARVRMSLDTVPEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSA
                     IEQEENVLMTARDDLEQQYARERELTGELLESRSDISRQSETHHLQQALHDIQQNQPL
                     LSVDVDVRTVAGVVADRTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRL
                     RAAKTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHT
                     VSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMR
                     DGEGREIDFRNTVILMTSNLGSDLLMQLLDEQPEATEASLHELLRPILRDHFQPALLA
                     RFRTVIYRPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTG
                     ARNVESLLNQQLLPVLSRQLLSHMAAKQKPQALTLAWSDEDGIVLVFDAQAEGESSCA
                     VS"
     gene            1866..>2866
                     /locus_tag="ACHK1J_005069"
     CDS             1866..>2866
                     /locus_tag="ACHK1J_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7914198.1"
                     /translation="MRSFLTSKADSVVQQLSGLSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPSRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYRVSGHSSSPYLTPGQVLETDG
                     "
BASE COUNT          608 a          773 c          871 g          614 t
ORIGIN      
        1 ccggaatctc tcagacccgt caccctgcgc acccttgacc tcggcctgct gcaggccggt
       61 gccggcgtga aaggtgaatt tgaacagcgc ctgaaaaacg tgattgatgc cgtgcagcag
      121 tcaccggctc ctgtactgtt gtttatagat gaagcccata cccttatcgg ggccggtaat
      181 caggccggcg gcgcggatgc ggccaacctg ctgaagcctg cgctggcgcg gggtgaactg
      241 cgtaccattg ccgccaccac ctggtctgag tacagacaat acctggaacg tgacgcagcc
      301 ctggagcggc gttttcagat ggtcagagtg gacgagccgg atgatgagac ggcctgtctg
      361 atgctgcgct ccctgaaatc ccgttatgcg gaacatcata acgtgcatat caccgatgag
      421 gcggtacgtg ctgccgtcac actgtcgcgc cgttatctga cagaacgtca gttaccggac
      481 aaggccgttg acctgctcga tactgctgcc gcccgtgtgc gtatgagcct cgacacggtg
      541 ccggaacagc tgacccgtat ccgttcacag ctcgcttccc tcggtatgga aaaacaggca
      601 ctgctggaag atattgccgt tggtcatcag aatcacggcg aacgtctgtc tgccattgag
      661 caggaagaga acgtgctgat gacagcacgt gatgacctgg agcagcagta cgcccgggag
      721 cgcgaactca ccggtgagtt acttgaaagt cgcagcgata tttcccgtca gagtgagaca
      781 catcacctgc agcaggcact gcacgacatt cagcagaatc agcccctgct cagtgtggat
      841 gttgacgtac gcactgttgc cggtgtggtc gcggaccgga ccggggtgcc gttatcttcc
      901 ctgatgaagg acgaacagac ggaactgttg catctggaaa aggatatcgg cagacgggtg
      961 gtcggacagg acgtggcact ggagtccatt gcgcagcgtc tgcgtgcggc gaaaacgggt
     1021 ctgacatccg gtaacggccc ccagggggtg ttcctgctgg ttggtcccag tggtgtggga
     1081 aaaaccgaaa cggcactggc gctggcagac gtgatgtacg gcggcgaaaa atcactgatt
     1141 accatcaatc tgtcggaata ccaggagccc catacggttt cccagcttaa gggttcaccg
     1201 cccgggtacg tgggatacgg tcagggcggt attctgacgg aggctgtgcg taaacgccct
     1261 tacagcgtgg tgctgctgga tgaagtggaa aaggcccacc gggatgtgct gaatctgttc
     1321 taccaggtgt ttgaccgggg ctttatgcgc gacggcgagg ggcgtgaaat tgacttccgt
     1381 aatacggtca ttctgatgac ctccaacctg ggcagtgacc ttctgatgca gttgctggac
     1441 gaacaaccgg aagccactga ggccagcctg cacgaactgc tgcgcccgat actgcgcgac
     1501 cacttccagc ctgcactgct cgcccgtttc cggaccgtga tttaccgtcc gctgacgcag
     1561 tctgccatgc gcaccattgt ggagatgaag cttgcacagg tctgtgaacg cctgcactgc
     1621 cattatgggc tgagcacatc ggttgatgaa cgtgtgtacg atgccctgac atccgcctgc
     1681 ctgctgccgg atacgggtgc ccggaatgtg gagagtctgc tgaaccagca gctgctgccg
     1741 gtactgagcc ggcagttgct gagccatatg gccgcgaagc agaaaccgca ggcgctgact
     1801 ctggcatgga gtgacgagga tggcattgtt ctggtatttg atgcacaggc agagggagag
     1861 tcgtcatgcg cagtttcctg accagcaagg ctgacagcgt tgtacagcag ctttcagggc
     1921 tatcacggta ccgggttcat gtgcatgaat gcagccattt tctggatgtg ttgcgttaca
     1981 gcgcggttga gtccctcagc cagccctggc gctatgacgt ggttgtcacc tgctcttctg
     2041 cggatattgc ctgtgacgcg ttactgctga agccggcgtc atttactttc cagaccccgg
     2101 tgtctgacgg aacaccggcc ctgccgctca gaacggtatt cggcgtggtt gagtcgtttc
     2161 gtcgcatttc tgcctcgaat gatgagaccc gttacgcact gacgatagtt ccccgtattg
     2221 cccttctgaa ctatacgaaa gggagcgaaa tctacctcaa tcagtcagtc actgaagtgg
     2281 ttgagcaggt attgcgtaaa catggtctgg aagggcagga ttttgagttc cggctctccc
     2341 gggagtatcc atcccgggag ctcatcaccc ggtggcggga aactgacctt gagtttatcc
     2401 ggcgcctgct ggcggaggtg gggatttact ggcgttatga aatggacagc cgtcttgagc
     2461 aggacatagt gattttccag gacagccagc agcagtatga atctggcatc acgctgccat
     2521 tgcgcaatca ggccgggatg agtgacagcg gacaggagag catctgggat attcagacca
     2581 cctgtaacgt ggtgagcgga agtgtggtca cccgggacta caactaccgt gaagcgctga
     2641 aacctcagga cagtgccgaa agtattttca gtaaggaagg gatcaccacc ggagaaacgt
     2701 accattatgc tgaacccttc ctgacggcag gtgacgctga aagcccggag accggggcat
     2761 actttgcccg tctgcgtcac gaacgtattc tgaatgcgca gtatcgtgtc agcgggcatt
     2821 cttccagccc ttatctgacg ccgggtcagg tacttgaaac ggacgg
//
LOCUS       DAZGBC010000319         2848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGBC010000319 DAZGBC010000000
VERSION     DAZGBC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.321"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..367
                     /locus_tag="ACHK1J_005070"
     CDS             101..367
                     /locus_tag="ACHK1J_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914199.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            558..947
                     /locus_tag="ACHK1J_005071"
     CDS             558..947
                     /locus_tag="ACHK1J_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO7914200.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRYGLVY
                     ELYQRRLSAAIDNFVSTLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            944..1870
                     /locus_tag="ACHK1J_005072"
     CDS             944..1870
                     /locus_tag="ACHK1J_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7914201.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIRQATGLSDDELKKITH"
     gene            1870..2103
                     /locus_tag="ACHK1J_005073"
     CDS             1870..2103
                     /locus_tag="ACHK1J_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914202.1"
                     /translation="MRVSQPGKMPGFLSSRSRANSMTLAERLIQKGFEKGALEVAREA
                     ACRLRDMGWPPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(2134..2385)
                     /locus_tag="ACHK1J_005074"
     CDS             complement(2134..2385)
                     /locus_tag="ACHK1J_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7914203.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIWRLENELGETAEDV"
     gene            complement(2419..2616)
                     /locus_tag="ACHK1J_005075"
     CDS             complement(2419..2616)
                     /locus_tag="ACHK1J_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914204.1"
                     /translation="MQGAPHPARLKKNDGTTMACRGHWCTTPAQVKTEKTTRHIQSKK
                     VKFLHFFTLFYRADLVTMMIK"
BASE COUNT          693 a          725 c          764 g          666 t
ORIGIN      
        1 ccggaatgaa aaaaagcagg gcgataaacc ctgcctctct ccgccgctgc ttcccggcca
       61 ggaagagcgg cagtcatcaa caacataagg agaaatattt gtgaggccat caattatctt
      121 tgcaacggct gaatatgtaa agcgtctgcg tgaagagtgc ctgcgggaga ataaacccct
      181 gcaccgccat acccgcgtca gacgccagga actggcaccg gatgagatta acccggacgt
      241 cctggcgatg ggcggtcata tcgccagacg ctgcagtgag cggaagcggg tgcgtatccc
      301 cgccatgaaa gtcagcgagt gggggcacct gctccgtgcg cttgaaattg aacgggtctg
      361 ccactgatta ccccctgccg tctgtctgtg caggtggcag gatatgttca ggtactttac
      421 caggaggaac cgtgagcaag atgaaaaaca tcatgacgtc tatgtcgcat ggtaccggat
      481 tcctcctgtt ccataaaaaa acagagaagg gaaacaggtt ctctttcatt cagggcccgg
      541 acgggcagga gataaaaatg aagtcttgtt ttgacactga caaagatttt cagatgtcat
      601 ctgatgaagt cactttcaat gctgacagcg ttcgctcaaa actggaacta ttatccgaag
      661 aactaatgca ccgttatggt cttgtttatg agctatatca gcgacgtttg agtgctgcta
      721 ttgataattt tgtgtctaca ttgccgacag aacaacatct gctggtcctg gaactggccc
      781 gtgaggagtt tgattacctt tccgctgaag agattgcaga agaaatacgc cgtgacgcag
      841 aacgtggcta ttgttgtcac ggctttgatc gcaattgctg tcctcttggt tgtggggatt
      901 tggacgatga ttgctgcgat gctgctgatt tgatggagga accgtgagta agaagaaaaa
      961 caccacaacg cccacgccgc atgatgccgc gttccggtca ttcctggcga accccgacgt
     1021 cgccagagat tttctggaac tgcatcttcc ggcggagtac cggcagttgt gcgacctgtc
     1081 cacgctgaag ctggaacccg cctcctttgt tgagccggac ctgcatcagt acgccagcga
     1141 tatcctctgg agtgtgaaaa ccaccggggg taaagacggc tatgtttata cgctcatcga
     1201 gcaccagagc accgaaaatc tgtacatgcc tttccgcatg ttacgttaca gtgtggcggc
     1261 gatgcagaga catctggagc agcacaaaac attgccactg gtgattccgg tgctgttcta
     1321 tcatggtgag cgcagcccgt acccgtacag catgaactgg ctggactgtt ttgaggaacc
     1381 ggcgcttgcg gctaaaatat acacaaagcc gtttccgctg gttgatatca ctgtcgttga
     1441 tgacaatgaa atcatgaacc atcgccggat ggccgcactg acgctgctga tgaagcatat
     1501 ccgccatcgt gacatgatgg agctgctgga caaactcccg caggtcatgg tggaaatttc
     1561 agacgagcag gtgcgtgttc tgattcatta catcgttaac gcaggggact ctgtatcacc
     1621 ggaatttatg cgggcgctgg ctgagcgtct gccgcagcat gaggataaac tgatgactat
     1681 cgcagaacgt cttgagcaaa aaggtcgcca ggaaggcagg atggaaggac gcatggaagg
     1741 acgcatggaa ggagcacttg aaaaagccct ggctattgcg tgccagcttc agaaaatggg
     1801 gatgacgccg gagcagatta ggcaggctac cgggctttcc gatgacgaac tgaagaaaat
     1861 cactcactga tgagagtatc acagcccggc aaaatgccgg gctttttatc cagcaggagc
     1921 agagcaaaca gcatgacgct tgcagaacgt cttatacaga aaggttttga aaaaggtgct
     1981 cttgaagtgg cgcgggaagc agcctgccga cttcgggata tgggctggcc gccggaacgg
     2041 attcaggagg caaccggact ttccggtgaa gaactgaaaa aactgtttcc tgatgagcag
     2101 tagcctggca ttcagccagg ctgtgaatca agctcacaca tcctctgccg tttcccccag
     2161 ttcattttcc agacgccaga tgatatctgc cttgcgcatc ccctgcatgt gaggcagcag
     2221 atcacggact gaaagtgcct gagtggtcct ccagacggtg acatccacct gttccgcatt
     2281 ttcccaggcg tgccatgttc tctgtgtcac gccaaattct ctggccgctt tttcgcgtga
     2341 ccagagcatg ctttttcgcc agattcgcag ttcccagccg gtcataaaac acctttgaaa
     2401 aagtgaaaaa acttcacttt attttatcat catagtcacc aagtcagcgc gataaaaaag
     2461 agtgaaaaag tgaagaaatt tcactttttt tgattgtata tgtcgtgttg ttttctctgt
     2521 tttcacctgt gcgggagtgg tgcaccagtg accacgacat gccattgttg tgccgtcgtt
     2581 tttcttcagt cgcgccggat gcggcgctcc ctgcacacct tacggtgtgt aaggccactg
     2641 cgcctgatgg cgcagctgtc tctgcctgtc tgccgccgca ctgcggcctg ttctccgcag
     2701 acggactttc cgggggtaca tacacgacca catcccgttt cagtcatcca ggggagagat
     2761 gtaccagcca caaaactttt tgccgcaggc gtcaaaaact tttgcgtctg ccccggacaa
     2821 ctgccccgtg acgtggtgag cgtccccc
//
LOCUS       DAZGBC010000320         2844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGBC010000320 DAZGBC010000000
VERSION     DAZGBC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.322"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..1438
                     /gene="glpT"
                     /locus_tag="ACHK1J_005076"
     CDS             80..1438
                     /gene="glpT"
                     /locus_tag="ACHK1J_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO7914205.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            1443..2519
                     /gene="glpQ"
                     /locus_tag="ACHK1J_005077"
     CDS             1443..2519
                     /gene="glpQ"
                     /locus_tag="ACHK1J_005077"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7914206.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(2743..>2844)
                     /locus_tag="ACHK1J_005078"
     CDS             complement(2743..>2844)
                     /locus_tag="ACHK1J_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914207.1"
                     /translation="KYCPEGVQTAFTQEQYDKLIRYINIFFTFPKLE"
BASE COUNT          706 a          600 c          763 g          775 t
ORIGIN      
        1 gcaactcaag aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca
       61 cgggccacgg aggctatcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt
      121 acctgccgcg gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat
      181 attctttggc tatgctgctt actatttggt tcgtaagaac tttgcgcttg ctatgcctta
      241 tctggttgag cagggattct cacgcggtga tttaggtttt gccctttcgg ggatctcgat
      301 tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt
      361 tttcctgccc gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc
      421 atgggcgacg tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca
      481 ggggatgggg tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc agaaagaacg
      541 tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct
      601 gctgttcctg ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc
      661 tttttgcgcc attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc
      721 ctgtggcttg ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa
      781 agcggaacag gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact
      841 gctgtggtac atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga
      901 ctggtcaccg acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc
      961 ctacttcttc tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga
     1021 taaagtcttc cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat
     1081 cgcgactatc gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat
     1141 gattgttatc ggcttcctga tctacggccc tgtgatgctg atcggtctgc atgcgctgga
     1201 actggcaccg aaaaaagcgg caggaacggc agcgggcttt accggtctgt ttggttacct
     1261 gggcggttcg gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga
     1321 tggcggcttt atggtaatga ttggcggcag cattctggcg gttatcttgt tgattgttgt
     1381 gatgattggc gaaaaacgtc gccatcagga attactgcaa aaacgcaacg gaggctaatg
     1441 gcatgaaatt gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg
     1501 gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca
     1561 gtggatattt gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg
     1621 attatctgga acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc
     1681 attacctcga tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg
     1741 gtcgttacta cgcgatagat ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag
     1801 gtttcgatat tgaaaacggt aaaaaagtgc agacttatcc agggcgtttc ccaatgggta
     1861 agtccgactt ccgggtacac acctttgaag aagagattga atttgttcag gggttaaatc
     1921 actctaccgg gaaaaatatc ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc
     1981 aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg
     2041 gtaaagacga taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga
     2101 atgagctgga acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg
     2161 actggaatga aacgcagcag aaacagccgg acggaagctg ggttaattac aactacgact
     2221 ggatgtttaa gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg
     2281 attaccatat gttgattgag gagacatcgc agccgggtaa tatcaaactc actggcatgg
     2341 tgcaagatgc tcagcagaac aagctggtag tgcatcctta taccgtgcgg tcagataaac
     2401 tgcctgaata cacaactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg
     2461 taaatgggtt gtttactgat ttccctgata aagcggttaa attccttaat aaagagtaat
     2521 gctgctactg caggatggcg ttcatgcgcc atcctctgct aaagcgaaat aatgctacta
     2581 ccggttattc ttttcattaa ccggtgcatc ctgtaaaaaa gatgccaaca taattttcgt
     2641 aaaactcatt cgttaaaaaa gctaacactg ggcattgtta ttctttaaac agtatgaata
     2701 ggtctcaata ataaattgta atgcagttta tactgtaaag tattattcta atttcggaaa
     2761 ggtaaagaaa atattaatgt atcttattaa tttatcatat tgttcttggg tgaatgctgt
     2821 ttgcacacct tcagggcaat attt
//
LOCUS       DAZGBC010000321         2826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGBC010000321 DAZGBC010000000
VERSION     DAZGBC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.323"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACHK1J_005079"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACHK1J_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO7914208.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHK1J_005080"
     CDS             complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHK1J_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7914209.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHK1J_005081"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHK1J_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO7914210.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          675 a          779 c          696 g          676 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgcgcc gtactcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtcagc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggctgg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gctgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactgatgca gattgcccga acgactttgc gtttatggcc
      661 ggaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggctaaat
      721 tgcagagtac aaccttgtta tactttgtta ttcaaaatca gttgcccgtt gctatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgtgg gaatggctgg aaatacttaa
     1381 ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attctgtata gcgagatgta
     1621 cacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccgacg gtggagaagt tcgacagcga taccagcgaa ccgtgatctt tatactgata
     1741 atttttcagc ggcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aagttgccat
     1801 ctggtgtgca gcctgagcgc gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcaatcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttgg tttcaagacc accaatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttcacc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcagttcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaattt
//
LOCUS       DAZGBC010000322         2812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGBC010000322 DAZGBC010000000
VERSION     DAZGBC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.324"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..2762
                     /locus_tag="ACHK1J_005082"
     CDS             114..2762
                     /locus_tag="ACHK1J_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
                     /protein_id="HFO7914211.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYADQFIHHDEYKHAVEIKDGEVK
                     DIDNALVITTGNGSYGVSVAGEGSNLAINNGVIVTTGGLYPSDNSSLSTALTASAIVS
                     EFGGAVILQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFSDGDNTFI
                     VTAGNHAVGVLACASPGSARACVSSLDEESTADTGNNENSAKAILDMAKGEITTHGTE
                     SYAAYANGTVVKSGDKLDYTNASVTLTNVDITTHGDNAHAIAARQGTVSFNQGEIYTT
                     GPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDVLSGSSLRSANEIL
                     YHKNETSNVTITDSEVSSAADVFINNIKGHLTVDTTNSKITGSANLSTDDNTHTCLSL
                     SDNSTWDIKADSTVSKLTVDNSTVYISRADGKDFEPTRLTITENYVGNNGVLHLRTEL
                     GDDNSATDKVVINGNTSGTTRVNVTNAGGSGAYTLNGIEIISVEGESNGEFIKDSRIF
                     AGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRPEAGSYVA
                     NLAAANTLFVMRLNDRAGETRYIDPVTEQVRSSRLWLRQIGGHNAWRDSNGQLRTTSH
                     RYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYSAGLYA
                     TWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSF
                     GLEAAKYTWIFQQQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEK
                     NSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNPAASV
                     WGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT          797 a          581 c          707 g          727 t
ORIGIN      
        1 tatttaaatt tcttgtgctt ttgtttttgt ctttaccctc cgttttggta atctgaactc
       61 ataggttatt tgaccaggaa tgtttgttaa tccaatgaat ggatggttaa tctatgaaac
      121 tgaaaaaact ccccgggttt agtttaggac ttattgctct ggcggtgggt aatgcatatg
      181 ctgatcagtt tattcatcac gatgagtata aacatgccgt ggaaataaaa gatggagaag
      241 taaaagatat tgataatgcg ctagttataa caacgggtaa tggaagttac ggtgtatctg
      301 ttgcaggaga gggtagcaat ttagccatta acaatggcgt cattgtaaca acgggcggtt
      361 tatatccttc tgacaatagt tctctaagca cagcattaac agctagcgca atcgtaagtg
      421 aatttggcgg tgctgttatt ttgcagggta aaaacacgat tgtcacaacg ggggattact
      481 caattggttt gctaagtcaa acgagtggaa acctgaatac tgataccata ataagagtca
      541 actctgacgg ttcggttacc ccctcttttt ctgacggaga taatactttt attgttactg
      601 cgggtaatca cgcagtcggc gttcttgcgt gtgcatctcc cggaagcgct cgtgcgtgtg
      661 tatcttctct tgatgaagaa agtactgccg atacaggaaa taatgaaaat agcgccaaag
      721 caatactgga tatggcaaaa ggggagataa caacccacgg aacagaaagt tatgccgctt
      781 acgctaatgg cactgtcgtc aagtctggtg acaagcttga ttataccaat gccagcgtta
      841 cattaaccaa tgttgatatc accacgcatg gggacaatgc tcatgccatc gctgctcgcc
      901 aggggacagt ttcatttaac cagggagaaa tttatacaac gggtcctgac gccgcgattg
      961 ctaaaattta taatggcggc acggtgacgc tgaaaaatac atctgcagtc gcgcatcagg
     1021 ggtctggaat tgtgctggag tcttccataa atggtcagga agcaactgta gatgttttat
     1081 ctggtagctc actacggtca gcaaatgaaa tcctctacca taaaaatgag acgagtaacg
     1141 tgaccattac ggatagtgag gtatcatcgg ctgcagatgt ttttatcaat aatattaaag
     1201 gtcatttgac cgtcgataca actaattcaa aaataacggg ttcagctaat ctttcaacag
     1261 atgataacac tcatacctgt ctgtcgcttt cagataatag tacctgggat attaaagctg
     1321 actcaacggt gagcaagctc acggttgaca atagtacggt ctatatttcc cgggcagatg
     1381 gaaaggattt cgaaccaaca agattaacaa taactgaaaa ttacgttggt aataatggcg
     1441 tattgcatct cagaactgaa ttgggtgatg ataattcagc tacggataaa gtcgttatta
     1501 atggaaatac ctctggaaca acccgagtta acgttaccaa tgctggcggc agcggggctt
     1561 atacgttaaa tgggatagag attatcagcg ttgaggggga atcaaatggg gaatttatta
     1621 aggattcgag gattttcgcc ggtgcctacg aatattcatt aacccgaggt aataccgaag
     1681 cgaccaataa aaactggtat ctgactaact tccaggcaac gagcggcggt gaaacaaact
     1741 ccggaggaag ttcagcgcct actgttgcgc ccacccccgt cctgcgcccc gaagctggaa
     1801 gttacgtcgc caacctggca gccgctaaca ctctgtttgt tatgcgtctg aacgaccgtg
     1861 cgggtgaaac gcgctacatc gatcctgtaa ctgaacaggt gcgttcaagc cgactttggc
     1921 tacgtcaaat tggcgggcat aatgcctggc gtgacagcaa cggacagttg agaacgacct
     1981 cgcatcgcta cgtctcgcag ttaggggccg atctgttaac cggtggtttt accgacagtg
     2041 acagttggcg tttgggagtg atggctggtt atgcccgcga ctacaactca actcattcca
     2101 gcgtgtcgga ttatcgttcg aaagggagtg tcagaggcta tagcgcaggg ctgtatgcca
     2161 cctggtttgc cgatgacatt agtaaaaaag gcgcatacat tgacgcctgg gcgcaatata
     2221 gctggtttaa aaactcggtg aaaggggatg aattagccta tgaatcctat agcgcgaaag
     2281 gcgcaaccgt ctcgctggaa gcgggttacg gctttgccct gaataaatcc tttggtctgg
     2341 aagcggcgaa atatacgtgg atcttccagc aacaggcaca ggctatctgg atgggcgtcg
     2401 atcataatgc gcatacggaa gccaatggct cacgtattga gaatgacgca aataacaaca
     2461 tccagacccg acttggtttc cgcaccttta ttcgtactca ggagaaaaac agcggtccgc
     2521 acggtgacga ctttgaacct tttgttgaaa tgaactggat ccataacagt aaagattttg
     2581 ctgtctcaat gaacggtgtg aaagtcgaac aagacggggc gcgtaatctg ggggaaatta
     2641 aacttggcgt aaatggcaat ctgaatccgg cggccagcgt ctggggtaat gtgggcgtgc
     2701 agctgggtga taatggctac aatgacaccg cagtgatggt gggcctgaaa tataagttct
     2761 gatccccgtt agctaaaata ccgcgtcgaa ttcatcgacg cggtaacata tt
//
LOCUS       DAZGBC010000323         2778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGBC010000323 DAZGBC010000000
VERSION     DAZGBC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.325"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            179..904
                     /locus_tag="ACHK1J_005083"
     CDS             179..904
                     /locus_tag="ACHK1J_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7914212.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            862..1539
                     /gene="bglJ"
                     /locus_tag="ACHK1J_005084"
     CDS             862..1539
                     /gene="bglJ"
                     /locus_tag="ACHK1J_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO7914213.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1577..2365)
                     /gene="fhuF"
                     /locus_tag="ACHK1J_005085"
     CDS             complement(1577..2365)
                     /gene="fhuF"
                     /locus_tag="ACHK1J_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO7914214.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDPTLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNVLSSLLAVYSDHIYRNQPMMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQHRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2506..2742
                     /locus_tag="ACHK1J_005086"
     CDS             2506..2742
                     /locus_tag="ACHK1J_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO7914215.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLTYADLSPDKWRIVILMGLLLTP
                     LMLYHKQLRHYVLLPSCLALSAGIMLMLMNLNQG"
BASE COUNT          742 a          597 c          699 g          740 t
ORIGIN      
        1 tttgatgcta accttctgac acatgcagtg gagttgttgt gcagcaggag tatgctgata
       61 tgaaagtgaa tgctaaggat aatttattcg cttaatctat taatttgctg gaaacattta
      121 aggaatgaat gtcctgaata aaccatcata atttttagac agtggatgtg gaggaaatat
      181 gttgccagga tgctgcaaaa atggaattgt tatcagtaaa atacctgtta tgcaagcagg
      241 gttaaaagag gtcatgagga ctcacttccc tgaatatgaa ataatatcca gcgcctctgc
      301 ggaggacctt accttattac aattacgtcg ttccggatta gtcattgctg atttagccgg
      361 tgaaagtgaa gatccacgtt ctgtttgtga acattattat tctttaatct cacaatatcg
      421 ggaaattcac tgggttttca tggtgtcacg ctcctggtat tcccaggcag tagaactgct
      481 catgtgccct acggcgacgt tattgtctga tgttgaaccc attgagaatc tggtcaagac
      541 cgtacgttcc ggcaatacgc acgcagagcg tatcagcgcc atgctgacct ccccggcaat
      601 gactgaaact catgatttta gctatcgctc cgtcattctc actctttcag agcgcaaggt
      661 actgcggcta ttaggtaaag gatggggcat caaccagata gcttcattgc ttaagaaaag
      721 taataaaact atcagcgccc aaaaaaacag tgcgatgcgt cgactggcaa ttcacagcaa
      781 cgctgaaatg tatgcatgga taaatagcgc gcagggtgca agagaactta acttgccttc
      841 tgtttatgga gatgccgcag aatggaacac agccgaatta agaagagaaa tgtcgcactc
      901 atagaaaaat gcgtcatgag tagtatcggt attgagagtt tattcagaaa gtttgcgggc
      961 aacccttata agctccatac ctataccagt caggagtcat ttcaggatgc catgtcgcgg
     1021 atctcgtttg cggcggtcat tttttctttt tctgccatga gaagtgagcg cagagaggga
     1081 ttatcttgcc tgactgaact ggcgattaag tttccgcgta cccggcgttt agttattgcg
     1141 gatgatgata ttgaagctcg gctgattggt tcattgtcgc catcaccgct ggacggtgta
     1201 ttaagtaaag cgtcaacgct ggagattttt catcaggaac tctttttgtc attaaatggt
     1261 gtacgtcagg cgaccgaccg actgaacaat cagtggtaca ttaaccaaag ccggacgtta
     1321 agcccgacgg agagagaaat attgcgcttt atgtcgcgtg gctactcaat gacacaaatt
     1381 gccgagcagc ttaaacgcaa tattaaaacg atccgtgcac ataaatttaa tgtgatgtcg
     1441 aaactgggcg tcagttctga cgcagggttg ttggaggccg cagatattct gttatgtatg
     1501 cggcattgcg aaacaagtaa tgtgttgcat ccatattaat ccgcatgatg ccgggtttac
     1561 ttccccggca gtgctttcat ttcagcgtac aatcgccaca ttgctgcaca tccggtaagc
     1621 gataacgctg gcagcaagtg cggcgcacca gcaggccgtc gcgtagcacc acggtacgcc
     1681 agagtggatt atcttcaccg gtagtaagcg ttttctcaaa aaagagggca tggcgcagcg
     1741 attcaaccgt cgcctcgccg agcagttgct tcatctcagt aagataccag ttgatcaaat
     1801 aaccggtatt actccagata agtttgccgt tgatctctcc ggtcgcttct agtgcctgca
     1861 caaccggaac cagcgcctgg ctgattaacg tttccattcg atgctgcggc gaatgtggtg
     1921 ttgcgttttt atcttcacat acatcgaccc agaaacaggc ggcgcgtccg gtttcgtgaa
     1981 actcaacgtg gaaatgttcc ggcgacacat ctaatgcttt ttcctgcgtc agcagcgcca
     2041 gcattaatgg cggcaccatc aggccgatat accactgcgc ccacagtgag atcagcggtt
     2101 tgttctcgcg gatcatcatc ggttggttgc gatagatatg atcggaatag accgccagca
     2161 gagaacttag cacattcggt gatgaccatt gcgccagcgt catggcgtta agtggggcag
     2221 gttcatccag gcggataaac tcgagcaaat gttcacgatg ttttgcgatc gtcgcccgca
     2281 cggcttgcgc aagcgtggga tcctgcggct ggagatgcgt tcgccagata acatcttcat
     2341 agagcggtgc ggaacgatag gccataatcg ggatagtaat ctaaatgata atgattgcta
     2401 atcctagcga taggtttacc cgatagcaag ggatttctct ggcttgcaaa tgataaaaat
     2461 tatcatatga tattggttat cattatcaat gaaagagata aaaccatgtt gcaacgtatg
     2521 ctgggcagtg gctggggagt gttgctgccg ggattgctga ttgcaggact gacgtatgcg
     2581 gatttatcgc cggataagtg gcggattgtg attctgatgg gattactgct gacaccgctg
     2641 atgttgtatc acaaacagtt acggcattac gttttgctac catcgtgcct ggcgcttagt
     2701 gctggcatca tgctgatgct aatgaatttg aatcagggat gaaaaatcaa ggaagaaaca
     2761 agaaaggaag taaagata
//
LOCUS       DAZGBC010000324         2763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGBC010000324 DAZGBC010000000
VERSION     DAZGBC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.326"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(65..430)
                     /locus_tag="ACHK1J_005087"
     CDS             complement(65..430)
                     /locus_tag="ACHK1J_005087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914216.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDTN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCSANDISHLYRAFMSVEKLTLSGRQVNV
                     LMMLLYGSNGTEGARYEVA"
     gene            complement(526..1002)
                     /locus_tag="ACHK1J_005088"
     CDS             complement(526..1002)
                     /locus_tag="ACHK1J_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO7914217.1"
                     /translation="MQIIIMTRDRYLEYGLMCVLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRCGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            complement(1065..1271)
                     /locus_tag="ACHK1J_005089"
     CDS             complement(1065..1271)
                     /locus_tag="ACHK1J_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914218.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(1399..2379)
                     /locus_tag="ACHK1J_005090"
     CDS             complement(1399..2379)
                     /locus_tag="ACHK1J_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7914219.1"
                     /translation="MSAYVNPEASNGVAVGSWASADALNSVALGTGAQAMHRYSVALG
                     QQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTRDTDAATVGQLNRKADE
                     VYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNTRGLLSQRGVLETHEER
                     LNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQEMATENRAAVSRNRQV
                     GERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQSAALAGLFQPYSVGKF
                     NATAAVGGYSDKQAVAVGMGYRFNEQAAAKAGTAFSDGDVSWNVGVNWEF"
BASE COUNT          712 a          808 c          578 g          665 t
ORIGIN      
        1 tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcatat cgtgccccct ctgttccgtt actgccataa agcagcatca
      121 tcagtacatt tacctgccgg cctgacaggg tcagcttctc caccgacatg aatgcgcggt
      181 acagatgact gatatcgttt gctgaacatc gctcagtaag atgtctgata tcataaaccg
      241 gactgtcaac cactccccag agaaaacgga gcgcttccgg agaagaaaaa taaagggtgt
      301 tcgtatcact tatattattc ttatacagaa aaccgacaat ttcagatatc cgggaagaag
      361 atattatcac cacattatct gtctgcatgc cgctgataat gaatttaccg ggattaatat
      421 actccttcat tccctccaga aaataactgt tatcacaaat aaaatgcata cccgttccac
      481 atgaaattta tcacgcctgg ccacataaca ttaattatcc cggatttacc ggtctgcggt
      541 gctgcagaga tatttaatac tgacgccgcg cagaccattt ttctccatcg cattgtaaag
      601 atgcgcacat accgtcttca tgcttatccc cagctggtct gccagaaagg taaaaccgta
      661 ctcatggaaa acaactccga agaccaccac catctcggta cgggtcagtg gtcttgccgt
      721 atgtccgaac agccacgccc cacaacggat ggcctgcctg atatctgtca ggcatctcac
      781 agaggaaaca ggaatgacaa gaaaacgacg cccacagaac atacagtatg taagcctttc
      841 cagattacgg tcacagagaa tcacataact gtcttcggga agtggcagac ggcgctttcc
      901 ggcatcaaac agttcacggc ctgtcgtgag cctgtatccg ttcagcacac acatcaggcc
      961 atattccaga tatctgtcac gggtcataat aataatctgc atactctcag acccttatct
     1021 cccggatacc ggctccgttt accattaccg gtataactga ctcatcattc ataacggaaa
     1081 ctgtctgccg catacatcag tgcccgctct tccagctctg acagactcag ctctttcagt
     1141 tccccttctc cttcagcaag aagacaacat tcaaaaacag ctgcatcgtc gcattccaga
     1201 cgaacctgca ccctgtcagc ccgaccgttt acagtaaata cctgcactga acttatgcga
     1261 tagttcagca tatacactct ccctctcagc tcctcaggaa taccccggaa ccgtcagtca
     1321 tgccaccggg gagaaaagca ggcagcgaat catgaccgca tacagaacgc tgcccgcccc
     1381 ggtgactcag acccgggttc agaactccca gttgacaccc acattccagg aaacatcacc
     1441 gtcactgaac gcagtacctg cctttgccgc agcctgttcg ttaaagcgat atcccatgcc
     1501 caccgcaaca gcctgcttgt cactgtaccc gcccactgcc gctgtggcgt taaatttacc
     1561 cacactgtat ggctggaaaa gaccggccag cgcagctgac tgggctgctg cccgcttcat
     1621 ttcctcatga ttctcattaa tctgacgctg ctgactgttc atacgacgct catgcgcctg
     1681 cagggtacgt gcctgagaag caatggcgcg actgttgcgt tcacccacct gacggttacg
     1741 gctgaccgca gcacggtttt ctgtggccat ttcctgtgta cgtaccagcc cttcctgtac
     1801 agtaattcgt tcaccggttc cctcaccgcg cgtcacataa ccatgactgt ccacagcaac
     1861 ggtacttccg gtatttatct gctgttgctg gctgttcagg cgctcttcat gcgtctccag
     1921 cacgcctctc tgggaaagca gtcccctggt gttacggttt atggccaccg tgttccggat
     1981 aatattctcc ctgttctctg cagcaaccgt atcggtatgg tcacgcgcct taagtgcttc
     2041 cgcggcaatc cgcccactga cgtcactgta cacctcatcc gctttcctgt tcagttgccc
     2101 gacagtggct gcatccgtat cccgtgtacc atcactgaca ccaccgagaa cccgggtttt
     2161 atccggtaca cgcggactgg ccgggtttgt gacacctgag tcatatccga tgtagacttc
     2221 attttcacgg gttgtaccgg actgctgacc aagagccact gaatacctgt gcattgcctg
     2281 tgcacccgta cccagtgcta ctgaatttaa agcatctgca gatgcccagc tacctactgc
     2341 cacaccatta gacgcctccg gatttacata agcagacatc cccacggatg tggaaaacgg
     2401 cgcatgcgca tacgatgcag agccaagagc tgtccctgcc ttcccggcat tcgcccatgc
     2461 ccccactgcc gtggagtcag tctctgatgc agaggctgtg tccggagacg cccaggactg
     2521 ccatcctgcg caaacgccgg agcaccggca atcagaaagg aaataagcga taaagtaata
     2581 ccgtgtcgat ttattttcat aaacaacctc attgatatac acaatatact ggcacagagt
     2641 taaattaata tataatcagg agagaagtga aagagtaccc ggtgcgaaaa tgaattaatc
     2701 tgagcagatt taacggtaaa cgaagcgcaa taaattaaaa ttaaaaaaca aaatcacttg
     2761 cag
//
LOCUS       DAZGBC010000325         2742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGBC010000325 DAZGBC010000000
VERSION     DAZGBC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.327"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..392)
                     /locus_tag="ACHK1J_005091"
     CDS             complement(<1..392)
                     /locus_tag="ACHK1J_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HFO7914220.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLE"
     gene            complement(545..997)
                     /locus_tag="ACHK1J_005092"
     CDS             complement(545..997)
                     /locus_tag="ACHK1J_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO7914221.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            complement(1023..2573)
                     /locus_tag="ACHK1J_005093"
     CDS             complement(1023..2573)
                     /locus_tag="ACHK1J_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFO7914222.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVHFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
BASE COUNT          800 a          650 c          490 g          802 t
ORIGIN      
        1 tcttccagac gggaaataat gttgtccaca cgaaccagct ggttttctat atcccgacga
       61 tgattatcag tgacaatacc actacgtgta cttttactga tgtcaatcag ataaacagga
      121 tccccttttt ttatcaactg tccttcatga acaaactgcc tgacaacaaa tccctgtaca
      181 cctgaatata tattgacagc tcttggccag gttgtgacct caccactgac attaacacgg
      241 cggctatagg taccaacaat aatgaacatc agaaatgccg taataaacac tatgcttccc
      301 agcatgatta accatagtgg tattccggga agtaatattg cccgtccctg ccacttcatt
      361 tttctgtttt ctaaagcatc ctgacgaaac aaaatagctc ctcttactca cacttttcct
      421 gtaatacctt cactggttaa gcgaaatgct ataatcaaag ccagaaaata cagagtcctg
      481 tcaaaatagt atcagcgatg catttacccc gtaatttaca attatctctg tatcttcaga
      541 ggtactacgt tttatgcagc cgctcaggga tgtatgtcac aattttcttt cgcctgctta
      601 accatcgtac ctctccttct ccccatggct gaagctgtat gagatagtca ataacttttc
      661 gaccaaagcc tggtttacaa cagaaatcca gaatccaaat ccttccatct tcattccatt
      721 cagacggatg caacctgaat tctggatccc tgagcaggcg cgcttctgta tcagcctcaa
      781 gatatgccca ggtaatatac gccagtggaa aacctcgtga atcaaacagg aatttaatct
      841 gattatgctg tatcgcatat tcagtccagt attgaatggt taacaccgga tacagactgt
      901 aattacgatt gtagagcata caactgaccg caaaaccgat ctgttcataa acagtccatt
      961 catcttttcc tctgatggtt tcccttatat aagacatgat tactatttcc cttatatatt
     1021 ttttacactg tgtttcttat cggttcaaga tatccaaatg catctctgga aaacttaagc
     1081 ataaaatttt tcatatcaga aattgcttct tgtaaaaaaa ggggatcatc gccatgatta
     1141 tccagcagga tggcaatttt ttcgcacata ttttctttcc tcagctcttc cctgggttgc
     1201 agccttttca tggtatgatg ttcaatttct ttcaggagta atgaggcatt tatctgacgt
     1261 ctggtactgc cttgcgtcag ccagtggatg gcctgggaaa aacactgttg tgcaacggtg
     1321 aaatgaacat cattcttgat gctactattc agtgcctgct tttgattatg cctatgatgg
     1381 attagcgtca caaccaactt ttgcaatggt gaaccatcct gattgacaat atcttgggca
     1441 tgtgatttct cgcagtattg attgagtaat gcaacaaaac tgttaaccag ttctttcaat
     1501 atatcaggta tctctttttg tgttataaat tctccaggaa tacccaacag ttctagataa
     1561 cagtaataac atgcttcatg aaaagttttc tgaaatatct cctcaacctt caaacgggct
     1621 gcaagagtga agtcagggtc aaacatatat tttccgatat acaagcagag atcagacagc
     1681 ccttgcataa gtaccatcgg ttcttttgcc agttcagcta atcttagttt atacccaaaa
     1741 tcagctgggg aatctcccgg aaacagacaa ctgagtccgt taaaatccat taatctgcca
     1801 tccattgcaa tattagaagg agaagttgtc aatctcagga aacgtgttcg acaaaatgcc
     1861 atttgccagg cctcacgacg tgccagttca cataacccct caatgcaata aatccgggga
     1921 tcagttcgtg cttcagcatc aatttcttcc ggtggtacag gtagatatcc tggcagcttg
     1981 tggatcacag atctaacccg acaggcatcg tgaattaaca gactggaata ctccggtttt
     2041 acttggaagt aaggtgcccg ttcaaaatgc gccggacgaa caacaggctc acgtaccaac
     2101 agggctcttc gtgatttcct accggagcgc tcaaatgcct tttcagtata gagatctgta
     2161 agtaaaaccg cccgaacccg cacagcacta taaggtaata tttgagccag tacttctccc
     2221 cacaaagcct catatattgc atgaacagcg ccgagtgcac cattagaatg acgttcgtca
     2281 gtaccttcgc caaccaacgg attacttcct attcctttaa cctggtagtt accatcaaac
     2341 ccacaacgtg caccacctcc attacgctga ataccactgc caccatagcg ctctgcatgg
     2401 aacactttgg cagaagctgt actgccagga tcgataatat aactgcaggc attttgcagc
     2461 tcgtctggat ttctgacatc ctctgcacac cagattaatg aggccttgat ctgcctggca
     2521 gtgaaaggca ccaggttttc attcagttca gaaagtgaac actgatgact catctggcag
     2581 tattctccgt ttcaattttc ataaaagagt gttcataaaa gagcaagtaa gtgtcctgac
     2641 agcttcttcc ttattacagt aaaactctat taattacaca taaattaaaa aattatttat
     2701 caacgactgt aaatcatatc ttcatcagta aagtgttgaa ca
//
LOCUS       DAZGBC010000326         2706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGBC010000326 DAZGBC010000000
VERSION     DAZGBC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.328"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..1601)
                     /locus_tag="ACHK1J_005094"
     CDS             complement(30..1601)
                     /locus_tag="ACHK1J_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO7914223.1"
                     /translation="METSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPALPS
                     ALRQSSARKPLPTTLPRETRVIRPEEECCPACGGELSPLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     DVELSRATLGRWTGAVAELLEPLYDILRQYVLMPGKVHADDIPVPVQEPGSGKTRTAW
                     LWVYVRDDRNAGSEMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRVLYESG
                     RITEAACMAHARRKIHDVHARVPTDITTEALQRIGELYAIEAEVRGCTAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWDGLNVYCGNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLTSQ"
     gene            complement(1621..1968)
                     /gene="tnpB"
                     /locus_tag="ACHK1J_005095"
     CDS             complement(1621..1968)
                     /gene="tnpB"
                     /locus_tag="ACHK1J_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7914224.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKIHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(1968..2615)
                     /gene="tnpA"
                     /locus_tag="ACHK1J_005096"
     CDS             complement(1968..2615)
                     /gene="tnpA"
                     /locus_tag="ACHK1J_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO7914225.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQVFTDWPLSGEYSCGQLARALFPGRYPSTPTVTQPVKAEKPRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPE
                     PDNKIPDPVQVEQINTPTDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
BASE COUNT          571 a          758 c          740 g          637 t
ORIGIN      
        1 taagcggctc gccagaaccg tattgatatt tactgagagg tcagatcaac tttccagggc
       61 aacagatcgc gtacccggtt tgccggccag tcctggatat gctcaatgac gtaacgcagc
      121 catttttctg gttccacatt gttcagacgg catgtgccga tcagcgagta caacaccgcc
      181 gcatgctcgc caccgctgtc ggaacccgcg aagagccagt ttttccggcc tacggccact
      241 ccccgtaagg cgttctctgc gatgttgttg tcgatttcca cccagccatt accgcagtac
      301 acgttcaggc catcccactg tttcagcagg tatgcgaacg cttttgccgt atccgagtga
      361 cgcgacagtg ttttcatctg agtctgtatc cagtcataca gtgactgcat cagtggcgca
      421 gctctggctt ttcttgccgc cagacgctgt tctgctgtac atccccggac ttctgcctct
      481 atggcataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt gggcactctt
      541 gcatgcacat cgtggatttt tctccgggca tgagccatac acgcggcttc cgttattctg
      601 ccggattcgt ataacacccg gtaaccaccg taagcatcgg cctgaagcac accgctgtaa
      661 ccggccagat gattttgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
      721 gccgggggca tttccgaacc ggcgttacgg tcatcacgga cgtagaccca cagccaggcg
      781 gtccgggttt taccactgcc cggctcctgg accggaacgg ggatatcatc ggcatggact
      841 ttaccgggca tcagcacata ctggcgcagg atgtcataca gcggctccag cagttcagca
      901 acggcaccgg tccagcgacc cagcgtggca cggctcagct ccacgtcctg gcgacggtat
      961 atttctgact ggcggtataa cggcagatgg tctgcatact tcccggtgac aacatgggcc
     1021 agaagccccg ctccggcata actgcgtgca atgggttttg aaggcaccgg tgcctgcaca
     1081 atatggtcgc accgacaaca ggccagtttc ggacgttgtg tttcgataac cttaaaggcg
     1141 ctgctgataa gctccagctg ctctgacaca tcacatccca gtggactgag ttcaccacca
     1201 caggccggac agcattcctc ttccggccgg atgacccggg tttcacgggg aagtgtggtc
     1261 ggtaacggtt tacgggctga agactggcgc agggcggatg gcagtgccgg gtcatattgt
     1321 tcacccagcg tttccgccat ttcttcctga agtgcgctga ttcgctcctg tgcttcctgt
     1381 atctgccgtt cggtttttgc acgaagtttt tctgagcttt taccgaactg catacgctgc
     1441 agtttcgcaa ccagcgcctt cagccggttg atttcagaag cataagccgc caccctctgt
     1501 gagagcaggc ggttgtattc ggccatctgg cggatggtgt cctgttgcgt ctgcaacagt
     1561 gcccgcaggc gggcgttctc atgagcaagt gaggtctcca tatcctcact ttacaacggg
     1621 ttatatgcgg attccagcgc gttccgttcg ttttggatgt ttccagtcga tgccttcaag
     1681 aagcatggac aactgagccg gagtaaggtg aatctttcca tcgcgggtga ccggccagac
     1741 gaagcggccc cgctccaggc gtttggtgaa gaggcacagt ccgtcgctgt cagcccacaa
     1801 cacttttatc tggtcacccc ggcgtccgcg gaagatgaac agatgcccgg agaacgggtc
     1861 atccttcagg acgttctgaa cttttgatgc caggccgtta aaaccatttc gcatgtcggt
     1921 gataccggca accagccaga tacgcgaacc ggcagggaga gatatcatca gtggctgctc
     1981 cctttcattt cgcggataag tgtctgtaat aacgccggtg tcagttcacc tttcagcctg
     2041 agagttccgg ccggcagaac cagctcgcag cacagactgt cggtcggtgt atttatctgc
     2101 tcaacctgca cggggtccgg gattttatta tccggctccg gagttaacgt cacgggaagt
     2161 agcgatgttt catgttcgcc gggcggctgc aggccgccgt tacgccagag atgccgccag
     2221 ttaaagagca ggttatcgtt gattccgttc tcacgagcta tttgtgcgac acaggcaccg
     2281 ggtaaaagag tctgctgaac aagtctccat ttaaactccg gtgaaaatcg gttccggcgg
     2341 ggtttctctg ctttcacagg ctgagtcacg gtcggggttg aaggatatcg ccccgggaaa
     2401 agagcccggg ccagttgccc gcaggaatat tcaccggaaa gaggccagtc agtgaatacc
     2461 tggtgaaatc gcctcaccag agaaaaaatg gcggttttgc tgacaccagt ctgcgcagaa
     2521 atgcgcgggt acgacagtga ctcgtcaaat ctcagctgta gcacacgcag gatcaattcc
     2581 ggattatctg ccagtaccgc ctttcgtgca ttcatcttaa atgtcctttt actgcgaaaa
     2641 tggacattag tatcggaaac aggaaaagga ggcgaaagac ggtttaaatg atgcgcttac
     2701 caacat
//
LOCUS       DAZGBC010000327         2613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGBC010000327 DAZGBC010000000
VERSION     DAZGBC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.329"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            182..1366
                     /locus_tag="ACHK1J_005097"
     CDS             182..1366
                     /locus_tag="ACHK1J_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7914226.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            1695..2276
                     /locus_tag="ACHK1J_005098"
                     /pseudo
     CDS             1695..2276
                     /locus_tag="ACHK1J_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            2363..>2613
                     /locus_tag="ACHK1J_005099"
     CDS             2363..>2613
                     /locus_tag="ACHK1J_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7914227.1"
                     /translation="MTGILLGQELRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYLEGSLTAVAAGEDVVPASELAAAIKQIKE"
BASE COUNT          745 a          633 c          592 g          643 t
ORIGIN      
        1 caccactttc acttccagat tcatctcccg aactccacga aactcaagta taatcacatc
       61 gttacaagca atctgaacat cctgaactcc accgaactct actgaactct ttctcaatca
      121 agctttcatt gggggcaccg gtgggggctc attatgttca gtcagtttca ggagccccca
      181 tatggcactt acagccagac aggtcgaaac agccagacct aaagaaaaag actataaact
      241 ctctgacgaa cgtggtttat atctgctggt aaaaaccacg ggagcccgct actggcggct
      301 taaataccgg atagcaggaa aagagaaaaa actggccctc ggcgtctatc ccgacgtctc
      361 ccttgctgag gccagaatca aacgtgacga tgcccgaaaa atcatctccg aaggtggtga
      421 cccgggcgaa aagaagcgaa aggaaaaact cactcagaaa atctctgcca ccaatacgtt
      481 ccatgccctc gctacggaat ggcaccagca taaatctttg tcatggtctg aaagttacgc
      541 cagaagcgta ctggaagcgc tggataaaga tattttcccg tatctgggca aacgaagcgt
      601 tacggatatc ctcccgctgg aaatgctgga aattctgcgc cgcatagaaa aacgtggctc
      661 gctggaaaaa ctgcgtaagg tacgtcaata ctgtaatcag atttttcgtt atgccatcgc
      721 caccggacga gccactgtca atccggcatc tgaactgacc agtacgctgg cggcgccaaa
      781 agctgcacac ttcccccacc tgagagcaga tgagctccct gtttttctca ggaagctcgc
      841 tgaatatcat ggcagtcctg ttacccgcat ggcgacaaat ctgctgcttc tgacaggcct
      901 cagaacgatt gagctacggt ccgctgaatg gtcagaaatt gattttgata atgccctgtg
      961 gacaatccct gaaagccgca tgaaaatgcg acgtaaacat gtcgtaccac tgtcacgaca
     1021 ggtcactgac attctgcaac aactcaaaac tttctccgga caataccggc tggttttccc
     1081 gggacgttgt gatatcaaca agccaatgag cgaagccagc atcaatatgg tgctcaaacg
     1141 tatcggttac gatggcagag caaccggtca tggttttcgt cacaccatga gtaccattct
     1201 gcacgaacag gggtttaatt ctgcctggat tgaaatgcag ttagctcatg tggataaaaa
     1261 ttccatcagg ggtacctata atcatgccct gtatattgat ggtcgccgtg aaatgatgca
     1321 gtggtatgct gactacattg attcgctttc tgagcaggta agttaagctg agaacacgtt
     1381 attctgattt tcactcactt cacgattatt cctccggcct gcaggttcgg aggattctta
     1441 tttttatgtt ttattttcag ataaagtttt tgacaaaacg tttattctgc atcagcaaaa
     1501 ttatcacaaa acaacgcaat cacagacaaa ttaacatttt tcagacgggt atattgagtg
     1561 cccacaatgt aagacaggca ggcatccggt gacatggtaa aatattaatc cagggcaaaa
     1621 acaaggcttg atacacaaca tcatgacttt tatgctctca gactatgcca ccacagattt
     1681 tacaggctca gaaaatgaat gacagattac gctttgaagt gaacgataac cagggctgtt
     1741 tcgtgttccc ggacgcctgg tttggctcgt tgctggatga gtttgacgaa ctaattgatg
     1801 catacgacag agatgaaatc agcgaaacaa gttatatcaa caaactcaga cgccttgccc
     1861 gacaggagcc tgactttatt gatgtacacg cttacctggc atgcgcattt ctggagcaga
     1921 atgccccgcg taaggcactg aacgctgctc ttaaaggact ggcagcaggt aatcgtctca
     1981 tcccggaaag tttcagtggt gaaatcatct ggatgcatcc cgaaaaccgg ccttatctgc
     2041 gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca gcgtcatcag gatgcagctc
     2101 tgctcactga taaaatactg gcatacaatc ctgaagacaa tcagggagca cgctggctac
     2161 tgggaccgga actgttacgt actggcgacc atgaacaggc acgccatgta ctgaaagcgc
     2221 atgcagatga attttcacct tactggtatg aactcggcct tctgcatttt ctgaaccggc
     2281 cccttgaaac gccagacagt ggatgtatct taaaataaga gataggcttg gatatatgtc
     2341 taacactagt tctaattttg agatgactgg aattttgctg gggcaggagc ttcgtaaacg
     2401 taaaactcct caggaaaaaa tcgccattat tcagcagaca atggaacccg gtatgacggt
     2461 gtcccatgtg gcgcgtttgc atggtattca gcccagcctg ttgtttaagt ggaaaaaaca
     2521 atatctggaa ggaagtctca ctgctgttgc tgccggagag gatgtcgttc ctgcctcaga
     2581 actggctgcc gctataaaac aaataaaaga gtt
//
LOCUS       DAZGBC010000328         2597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGBC010000328 DAZGBC010000000
VERSION     DAZGBC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.330"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHK1J_005100"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHK1J_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO7914228.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="ACHK1J_005101"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="ACHK1J_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7914229.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..>2597
                     /locus_tag="ACHK1J_005102"
     CDS             1077..>2597
                     /locus_tag="ACHK1J_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO7914230.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVDLSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPA"
BASE COUNT          622 a          715 c          724 g          536 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggatc
     1741 tgagccgtgc cacactgggg cgctggacag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccggacagc ccggctgtgg gtctacgtcc
     1921 gtgatgaccg taacgccggt tcacagatgc ccccggcggt ctggttcgcg tacagtccgg
     1981 accggaaagg tatccatcca caaaatcacc tggccggtta cagcggtgtg cttcaggccg
     2041 atgcttacgg tggttaccgg gcgttatacg aatccggcag aataacggaa gccgcgtgta
     2101 tggctcatgc ccggagaaaa atccacgatg tgcatgcaag agcgcccacc tacatcacca
     2161 cggaagccct gcagcgtatc ggtgaactgt atgccatcga ggcagaggtc cggggctgtt
     2221 cagcagaaca gcgtctggcg gcaagaaaag ccagagccgc gccactgatg cagtcactgt
     2281 atgactggat acagcaacag atgaaaacac tgtcgcgtca ctcagatacg gcaaaagcgt
     2341 tcgcatacct gctgaaacag tgggatgcac tgaacgtgta ctgcagtaat ggctgggtgg
     2401 aaatcgacaa caacatcgca gagaacgcct tacggggagt ggccgtaggc cggaaaaact
     2461 ggatgttcgc gggttccgac agcggtggtg aacatgcggc ggtgttgtac tcgctgatcg
     2521 gcacatgccg tctgaacaat gtggagccag aaaagtggct gcgttacgtc attgaacata
     2581 tccaggactg gccggca
//
LOCUS       DAZGBC010000329         2595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGBC010000329 DAZGBC010000000
VERSION     DAZGBC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.331"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(103..990)
                     /locus_tag="ACHK1J_005103"
     CDS             complement(103..990)
                     /locus_tag="ACHK1J_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914231.1"
                     /translation="MSTSSFIFAILPVILMICIITSLFWVVIYARGKWTFSSGITVFR
                     YLQQYRWFYFGNISLLSLSVILTTRVYDTEYPLQVYICIYIPPVMILTSLLTILTIKE
                     ATRTYKTYKSNRLTPVSVIAYAGLGYMFCFTTGIYELSSTRLNPVISDMLMSFLSRLS
                     IMLPVTLAEIAVIYLLLLALHFPDGVSARGFSVTRYLRHNDWFYYINTGNAILALILS
                     TWIYHSSISLPAALFTGALSIFFNTTIPASLSVIEHTPSDTDSKIWDTRKTSPATVLY
                     CSGTGYVIGFIVNPSAAIS"
     gene            1327..1572
                     /locus_tag="ACHK1J_005104"
     CDS             1327..1572
                     /locus_tag="ACHK1J_005104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914232.1"
                     /translation="MNEHEAPDYREIFFMGNASQFVGKNRKRKESKKSDKGNVMKSVC
                     KILIEVSPCRMQNVTGRDDYLSWAIKKRLSEMLNTRS"
     gene            complement(1606..1941)
                     /locus_tag="ACHK1J_005105"
     CDS             complement(1606..1941)
                     /locus_tag="ACHK1J_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914233.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSVTGKK"
     gene            complement(2057..2299)
                     /locus_tag="ACHK1J_005106"
     CDS             complement(2057..2299)
                     /locus_tag="ACHK1J_005106"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO7914234.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(2380..>2595)
                     /locus_tag="ACHK1J_005107"
     CDS             complement(2380..>2595)
                     /locus_tag="ACHK1J_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HFO7914235.1"
                     /translation="ISGGNLAEQAVTAALAYRTQAPLIDSLLKEVGLSGEGLTALTRP
                     LVMPAPEKVPDVSEPVTAPDNSEPQQV"
BASE COUNT          771 a          476 c          596 g          752 t
ORIGIN      
        1 gtaaaagtaa agtctatagc tacttctggt tttttatctg gttttttgtt tttaactgtg
       61 ctgtttattc ttctctgaag gcaataaata taaaagatat aatcagctaa tggcagcaga
      121 gggattcacg ataaaaccaa tgacgtaacc tgttcccgag caataaagga ctgttgccgg
      181 ggatgttttt cgtgtatccc atatctttga atctgtatca gatggcgtgt gttcaatgac
      241 cgataagctg gccggaatgg tcgtgttaaa aaaaatactt aacgcgcctg taaataaagc
      301 agcgggaaga gaaatgctgg aatgatatat ccatgtggag agtatcagtg ccagaatagc
      361 atttcctgta tttatataat aaaaccagtc gttatggcgc agataccggg taacggagaa
      421 ccccctggcg gacacgccat ccgggaaatg cagcgccaga agcaggagat aaataactgc
      481 tatttcagcc agtgtaacag gcagcataat ggaaagacgt gacaggaatg acatcagcat
      541 gtcacttatg acaggattaa gtcgtgttga agataattcg taaatgccgg ttgtaaaaca
      601 gaacatataa cccagtccgg cataagcaat aacggaaacc ggcgtcagtc tgtttgattt
      661 gtatgtttta tacgttctcg ttgcttcttt aatggttaaa attgtcagca gggatgtgag
      721 aatcataacg ggtggtatat atatgcagat ataaacctgc aaaggatact cagtatcgta
      781 tacccgggtt gttaatataa cggacagact cagtaaggat atatttccga aataaaacca
      841 gcgatactgt tgaagatacc ggaaaacagt gatgcctgaa gaaaatgtcc atttgccgcg
      901 agcataaata acaacccaga ataaagaagt aatgatacat atcatcagta tgacgggtaa
      961 tattgcaaaa ataaatgatg atgtgctcat tattaaatct ccatcagcat ggtgatgctt
     1021 aaatttttac ggttgtgagg tgcgtaatca atatcatgtg tagttgtgaa taatagcgtc
     1081 agcaacatat ataatattgc aggtgccaaa aaagaataaa aaaacagaga gcaggatgac
     1141 agaggttgtt gtcacgcctc cttcatgtgg tgatttatcc tgagaaatta gctccccggc
     1201 cactgtcgca gttgcaattg atgtgagaga aatatcagtg ataaatccga aaagaatcat
     1261 ggttagtgtt atattattaa agggaatata cgtaacaaga ctgatactga gaaccagaat
     1321 ggttatgtga atgagcatga agcgcctgat taccgggaaa tattttttat ggggaatgcc
     1381 agtcagtttg tagggaaaaa tcgtaagcgc aaagaaagta aaaaaagtga taagggtaat
     1441 gtaatgaaaa gtgtatgcaa aatactcata gaggtatctc cgtgcaggat gcagaatgtg
     1501 acaggcaggg atgattattt gtcctgggcc ataaagaaaa gactaagcga gatgctgaat
     1561 acccgttcat aagagtgaat tgcatttcag aactggagca ttattttatt tcttgcctgt
     1621 tactgaaaca tttccggtta tgatttccga atggcaggta tcaataaaat cacttttccc
     1681 cgattgtgtg tcaaagtcaa cgtgggtgaa cctgtgtaca tcctgccata tggcgaaaca
     1741 gacaggacag gtaacaggta gtttgccttc agctattttt tcgcagcttt caaccagttg
     1801 ctgcgccttg ttatatctga cgttatagcc atttgtgcaa agcgtttcat acccatgagc
     1861 tcctgtaact atatgtgtgg cgattctttc gtggactctc ccattaatta ataattgttc
     1921 acgcgttgtg attgttctca tgcctgtttc tctgttggtc ataacataaa ataatctgca
     1981 ttcctgaaaa atatcgcggt tgtgaaatat ttagttttta tccccctttt atgggggtaa
     2041 aaagcaggct gtcaggctat aactgaacgc ggtttctgga ttttggtgct aatgtcacat
     2101 ctttccattc acagctgctt ccgggtatac gctctttgcg tcgtattata cgggagttct
     2161 gacacacaat aattaagcca ttgtctgccg tctgaaatgg cattatagcg atgtttatat
     2221 cttctgttgc aggaatataa ctgactttct tttcacatgt attggtgtat atgacaactc
     2281 tgaatttttc tgcggacatt ttcaatcctc ttttaaacca gaatatcccg tgagggcatt
     2341 ctttgtgaaa aagatgccgg aaaaattccg gcacggggat taaacctgtt gtggttctga
     2401 gttatccgga gcggttaccg gttcgctgac atccgggacc ttttccgggg ctggcatgac
     2461 cagtggacgt gtcagggctg taagtccttc accggataac cccacctcct tcagcaggga
     2521 gtcgatgagc ggagcctgtg tacggtaagc cagagcggcg gttaccgcct gttcagcgag
     2581 atttccgcca cttat
//
LOCUS       DAZGBC010000330         2538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGBC010000330 DAZGBC010000000
VERSION     DAZGBC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.332"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..682
                     /locus_tag="ACHK1J_005108"
     CDS             <1..682
                     /locus_tag="ACHK1J_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO7914236.1"
                     /translation="TKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETK
                     YILDQLEMKYPDLLKEILRYITVQRISEVIQRLIQERISVRNMQLVMEALALWSPREK
                     DIITLVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDAS
                     EAEMILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENV
                     PVNILKTI"
     gene            714..2006
                     /gene="sctN"
                     /locus_tag="ACHK1J_005109"
     CDS             714..2006
                     /gene="sctN"
                     /locus_tag="ACHK1J_005109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFO7914237.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1996..2454
                     /locus_tag="ACHK1J_005110"
     CDS             1996..2454
                     /locus_tag="ACHK1J_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO7914238.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYERLKTRIKKQLLDQMLYQDELEQEEKYNGRSQEN"
     gene            2432..>2538
                     /locus_tag="ACHK1J_005111"
     CDS             2432..>2538
                     /locus_tag="ACHK1J_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO7914239.1"
                     /translation="MDEVKKIEHGKILNTDTPDNSASDLDSFLKKNKKN"
BASE COUNT          834 a          446 c          581 g          677 t
ORIGIN      
        1 tacgaaaagt gatgctcaaa aattaacaaa aattggctgt tatactcgca cggccatgga
       61 tgaaatgtat aatcatttat ctgtatgtct ggctcataat attaatgagt actttggtat
      121 acaagaaaca aaatatattc ttgatcaatt agagatgaag tatcctgatc tattaaaaga
      181 aatattaaga tatataaccg tgcagcgcat ttcagaggta attcagcgtc tgatccagga
      241 gcgaatttca gtgcgcaata tgcagttagt tatggaggcc ttagcattat ggagcccacg
      301 agagaaagat ataatcacac tcgtcgagca tgtacgaggt gcattaggtc gctacatttg
      361 ccataagttt tcatattctg gagaaattaa agcaattgtg atttcgcctg aaatagagga
      421 cagaatcaga gatggtgtca ggcctaccgc tggcggtact tttcttaatc tcgatgcatc
      481 cgaagctgag atgattctgg ataattttaa actggcactt tctggaatca atattcctat
      541 taaagacatt attttactgg gatctgtgga tattcgtcgt tttatcaaaa aacttattga
      601 atctagttat cgagacctcg aagtactttc gtacggggaa ctaacggaaa atgttcccgt
      661 taatattctg aaaactattt aggataatgt cttgcgatat ggagcatgct ggtatgaaaa
      721 aattaaaatt attaaataaa tacagctact tacatagcat aaacggctct ctgattgaag
      781 cagaacttga cgacgtttct gtgggtgaag tatgcgaaat ttatgcgagc tggcaagcaa
      841 atgaacgtat agccagggct caggtcgttg gctttcgcaa tggaaagaca ttattaaacc
      901 ttataggcag ctcagtgggg cttacacgta ctgcagtttt gaaaccaacg ggagagcagt
      961 taaccataca gatcagtgat gcctttcttg gttcggtttt gaatgcctct ggtcaaatta
     1021 tggaaaggtt tgtcccggac actccaggcg atagaggaaa tttacgcctg attgatgaac
     1081 tgccaccgtc gtatcaggaa cgtcgagtta ttaatacacc actagagaca cgcataagag
     1141 tcattgatgg cgtactgaca tgtggcattg gtcaacgtgt tgggattttc gcatcggctg
     1201 gctgtggcaa aacggtttta atgcatatgc tcgtaaacaa tactgaagct gatgtgtttg
     1261 ttattggctt gattggcgaa cgtggtaggg aagttacgga atgcgcggaa tcgctgaaac
     1321 aatcagtaaa tgcggcaaaa tgcgtattgg tttatgccac ctctgatttt tcgtcagtag
     1381 atcggtgtaa tgccgccctg atggcgacaa ccgtggcaga gtattttaga gatcgaggaa
     1441 agagagttgt actttttatt gactcaatga cgcggtatgc acgagcacta cgtgatatga
     1501 aactggcagc gggtgaacca cctgccagac gtggttatcc tgcttctgtt tttgacagtt
     1561 tgcctcgctt actggagagg ccgggaccaa cactaaaagg aagtatcacc gcgttttata
     1621 ccgtgttact ggaaggggaa gatgaatcag acccactggg tgatgaaatt cgctcaatcc
     1681 ttgatggcca tatctatcta agccgcaaac tcgcgggtca aggtcactat ccggcaatag
     1741 atgttctaaa aagtgtgagt cgagtatttg gtcaggtcac tgatgaaaaa cacagagata
     1801 atgctgcacg ggtaaggaag atcctcacaa ctttggaaga tctacaggtt tttattgact
     1861 taggggaata tcgcgctggg caaaatgctg aaaatgattt tgctatgaat gcacggccta
     1921 aactcacaaa ttggttgaaa cagtcagtta atgaaaaaat gccaatgagt gaaactctaa
     1981 aagagcttga acgaattgtt aagtaaagtt aatcgactta taagaagaac agcgcagagc
     2041 ctggctgcat gtgaagcatc attacaaaag ttaaatgcag aaaaagaaaa gttagccgaa
     2101 aaggaacgcc tgtatgatat gcaacttaaa aacctgcagt cactgttaga tatgaaagag
     2161 ttactcggcg aagttgtatt tcgccaggat atattttact cgttacgtaa agttgcagtc
     2221 attcagcagc agatcgctga gataaatctt gaaaagcaaa aaatagcaga gcggagaaaa
     2281 atacttaaca aggaaatagt tcaacagcaa gcacaaagaa aacactggtg gctaaaagga
     2341 gaaaaatacg aacgactaaa aacgcgcatc aaaaaacaac ttttagatca gatgctttat
     2401 caagacgagc ttgaacagga ggaaaaatat aatggacgaa gtcaagaaaa ttgaacacgg
     2461 taaaatttta aacacagaca cacccgataa ttctgcttcg gatttagaca gtttcttaaa
     2521 gaaaaacaaa aaaaataa
//
LOCUS       DAZGBC010000331         2514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGBC010000331 DAZGBC010000000
VERSION     DAZGBC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.333"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..217
                     /locus_tag="ACHK1J_005112"
     CDS             <1..217
                     /locus_tag="ACHK1J_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO7914240.1"
                     /translation="TAVSSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIE
                     HYNETHPHSALGYLSPREYRRQRVTST"
     gene            257..736
                     /locus_tag="ACHK1J_005113"
                     /pseudo
     CDS             257..736
                     /locus_tag="ACHK1J_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            879..1108
                     /locus_tag="ACHK1J_005114"
                     /pseudo
     CDS             879..1108
                     /locus_tag="ACHK1J_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1095..>2514)
                     /locus_tag="ACHK1J_005115"
     CDS             complement(1095..>2514)
                     /locus_tag="ACHK1J_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7914241.1"
                     /translation="TIACGYTEKNATGNVLLLFLLLLLAHRNTLTSITALLFLFCCAL
                     YAPAGMTYGKINNSFIVALLQTTADEAAEFTGMIPVYHFLVSAAILVFMVIFWRTHHR
                     GHRNWLALLLFVLCSVNSWPLRMVKGTVVGTTDTLREMQRYEQLSQHRADSWKILPGA
                     PLYDTIVIVTGESVRRDYMSVYGYPVPTTPWLNTAPGLFIDGYTSAAASTVPSLSRTL
                     IYDYEQNPDSGNNVVALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKKGS
                     FASRKTDDMLLLQETERALADKSSPKVIFLHMIGSHPNPCDRLNSWPNHYLEQYPRKI
                     ACYLASISKLDNFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSANPVHHDGHVQGGYS
                     VPLIITASDITSHQSVSRKISARHFAGIFQWLTGIRTENIPPFNPLTDEDNEPVMVFN
                     GERNVPADSLKPQPLILPVRGK"
BASE COUNT          704 a          585 c          593 g          632 t
ORIGIN      
        1 gacggcagtc agtagccctc agagcaatgg aatggcagaa aggttcgtga aaacgatgaa
       61 agaagactac atcgcgttca tgccgaaacc gaatgtaaga acggcattgc ataaccttgc
      121 agtggcgatc gagcattaca atgaaaccca tccgcatagt gcgttaggtt atctctcacc
      181 aagggaatat cgacgtcagc gggtaacgtc aacttaagat acaacgactg tctgaatatc
      241 gtgggtcaag gacagccttc tgcattttct taatggggaa ctggtaaaag ctgcaactgc
      301 atttcgtcgc ggctttgcgg caaatactta catcgcagaa atactgtgcg gaaatctgca
      361 tccatttcca cttgctgtat ggcataactt ttcaggcggt ccggatactg ctgaagatta
      421 ttatgccaca taccacccgt tatgggggca atatccggaa gcattgctgt ttgtaaactg
      481 gctctataat cattcctctg tgctgcatga acgggcagaa atcattaaat gcgccgaaat
      541 gctgatgcag gaagatgatt tcgaaatatg cgaaagtatt ttaagacagc aggagaagtt
      601 gcgtgaaaga attggtgaga cgctttctga gaaaattgta cagaaatgca gaaatatgaa
      661 tggtgaatat gtctggccct ggatattgcc gttttcagcg gcaggcatga aacatactgg
      721 catacagtat cagtagatat tgcattagtg tatcctgcac acaagtaata atttatccac
      781 caataataac actgttaatg tccccttccc ctggttgtca gccaggggtt atcttctgaa
      841 tatttctttt gaaaaggata acacagtaaa ttatttttat gaattatccc atggactcat
      901 taacaccctt tcataatgct ttattgtcaa acacgttatg gctgacatca aaaaaaccgg
      961 atttcctctg ccagcgggta atcacctccc cggtgttttc ggttggtctg gttactccag
     1021 tctggtaatt agcaagataa tcgatataaa cagtggaaaa ctcatcgtac ataatctggt
     1081 gatgaacatt acgcttattt tcccctgacc ggaagaatca gaggctgcgg tttcagactg
     1141 tctgccggta cattcctctc cccgttaaaa accataacgg gttcattatc ttcgtctgtc
     1201 agcggattga atggcggtat attttcagta cgaatgccgg tcagccactg aaaaatacct
     1261 gcgaaatgac gggcactgat tttcctgctg acggactgat gagacgtgat gtcactggcg
     1321 gtaataatca ggggaacgct gtagcctccc tgcacatgac catcatgatg aacaggattg
     1381 gcactatcgc tgaccgacag cccatggtca gaaaagtaaa gcatggcgaa atgacgggaa
     1441 tgccggcgaa ggataccatc aagctgaccg agaaagttat ccagtttact gatgctggcg
     1501 aggtaacagg caatctttcg gggatactgc tccaggtaat gattcggcca ggagttaagt
     1561 cggtcacacg gattcggatg agagccaatc atgtgcagga aaatcacctt cggagaggat
     1621 ttatccgcca gcgcacgttc tgtttcctgt aacaacaaca tgtcatccgt tttacgggaa
     1681 gcaaagctgc ctttcttgag gaaaacggta tgctccgcat cagaagcaat aacagagatg
     1741 cgtgtatcat gctcccccag ttttccctga ttggatatcc accatgtgct gtatcctgct
     1801 tttgctgcca gcgctaccac gttgttgccg gagtcagggt tctgctcata gtcataaatc
     1861 agtgtccggc tcagggaagg tacggtactg gctgctgccg atgtatagcc gtcaataaat
     1921 aaaccgggag ccgtattcag ccacggtgtg gttggtacgg gataaccata taccgacata
     1981 taatccctgc gcacactctc accagtgaca atcacaatcg tgtcatacaa cggtgctccc
     2041 ggcaggattt tccagctgtc agccctgtgc tgactcagtt gttcataacg ctgcatttca
     2101 cgcaatgtgt cagttgtccc cacaacggtt cctttaacca tccgcaacgg ccagctgttt
     2161 acagagcata atacgaacag cagcagtgcc agccagttac ggtgaccgcg gtggtgtgtc
     2221 cgccagaaaa taaccatgaa caccagaatc gcggcactga ccagaaaatg ataaacagga
     2281 atcatcccgg taaactccgc agcctcatcc gccgtggtct gcaacaacgc gacaataaaa
     2341 ctgttgttga ttttaccgta cgtcataccc gcaggcgcat acagtgcaca acagaacaga
     2401 aataacagag ctgtaatgga tgtaagggta tttctgtgtg caaggagcag aagaaggaac
     2461 agcagcaaca cattcccggt ggcattcttc tcagtgtatc cgcatgcaat tgtg
//
LOCUS       DAZGBC010000332         2511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGBC010000332 DAZGBC010000000
VERSION     DAZGBC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.334"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2214)
                     /gene="ypjA"
                     /locus_tag="ACHK1J_005116"
     CDS             complement(<1..2214)
                     /gene="ypjA"
                     /locus_tag="ACHK1J_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO7914242.1"
                     /translation="MNRISRYYFHRSVLSLLIAAMIYAPPGMTAFTSNVIGVVNDETV
                     DGSQRVDERGTTNNAHIINHGNQEVYGGISNGSVIDTGGHQEVSGHGSYQGQANNTVI
                     NGGSQTISEGGISTGTIINDKGTMSVLTNAKADATRIDNGGAMDVAGNATNTIINGGT
                     QNIYNHGIATGTNINSGTQNIKSGGKADTTNISSGSKQVVEKGGTATGSNIRAGGTLI
                     VDTGGIAHGVYLDTGSALVANTGAGTDIDGYQRSSHFTITGGRAEHVVLENTGELTVV
                     AQTSAVDTIVDAGGKLIVHEEAVAYTTRLNNGGTLDVREKGSATGILQSTQGALVATT
                     RATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTTSAKTQVNTGGREIVKTKA
                     TATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTVNDNGKATD
                     IVQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNMLLENGGNLLVLAGTEAHDST
                     VGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGTAIVYAGTLAD
                     ASVSGATGSLSLITPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLTAASRGSVW
                     LNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTTNELSGSGNFY
                     LHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDASFTLGN"
     gene            complement(2279..2398)
                     /locus_tag="ACHK1J_005117"
     CDS             complement(2279..2398)
                     /locus_tag="ACHK1J_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO7914243.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2359..>2511)
                     /gene="ymgJ"
                     /locus_tag="ACHK1J_005118"
     CDS             complement(2359..>2511)
                     /gene="ymgJ"
                     /locus_tag="ACHK1J_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO7914244.1"
                     /translation="LVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTAFKKCLFLMR
                     DIIISS"
BASE COUNT          557 a          689 c          580 g          685 t
ORIGIN      
        1 attgcccagc gtaaacgaag catcccctcc ccctgttttc accagcgtca tcgcatcgtc
       61 agactgtggg ctgacgccgg tatcctgaac aaagatttta aaattaccag tggcgttgtt
      121 gttgacgacc agttgatcgc cccgggagcc agcaacgttg gtatgcaggt agaaattacc
      181 gctaccggaa agttcattgg ttgtcagcgt attgtagata ccgttagttg tggcaggcgc
      241 tgctgtttgt gctgataaat aaacattacc gtcgttaagt agcaaactgt ttactctata
      301 ctcgcagttg ctggtgcctg cacaggaatt attgctgtta agccagacac tgccccggct
      361 ggcagccgtc aggtcggcaa gcgtggtatc gacgccattt cccagagtca atgtcgcgct
      421 atcggtaatc cggaccgccc cttcaagttt tactggcgta acattatccc gtggcgtgat
      481 taacgacagg cttcctgtcg cgccactgac cgatgcatcc gccagcgtac ctgcgtagac
      541 gattgccgtc ccgccagcga cctggagatc ttctgcccgg gccagaggct gaaactcatc
      601 ttttgaccgc ccaagggtat attgcccgcc agagttaacc tttgtggcgg agtcctgacc
      661 caggttttgc attgcaccac ccttgccaac cgttgagtca tgggcttcgg tacctgctaa
      721 taccaataaa ttaccaccat tttccagcaa catattggtc gctaaatttc cggcaatgga
      781 aaaggtgccg tactggtgag taccgctgat ttcaataccg ttagccgtgc tcgtctggag
      841 agcggcaccg ctgttctgga cgatatctgt cgctttgcca ttatcgttga ctgtcagcgt
      901 accgccttca ttgatcgttg tttttattgc ctcaccgtta gccgaaactg tttgtattcc
      961 gccgtcgtta atcgttgtct cattcgccac accctcgacg atttgttcgc cgccggtgag
     1021 cgtcgtgcct gtcgcagtgg cttttgtttt gacgatctcc cgtccgcccg tattgacctg
     1081 tgttttggcg gaagtggtgt ctgactccac ggttaacacg ccgccatttg ccagcaggat
     1141 attgttcgcc gcaccctgct cgatgctgaa cgcgacgcca tcagcgcgtg tccctgtgac
     1201 acgcgtcgcc ctggtggttg caaccaacgc gccctgagta ctctgcagta tccccgttgc
     1261 gctgcctttc tcccgcacat cgagtgtgcc gccattgtta agtctggtgg tatatgctac
     1321 ggcctcctcg tggacaatca gcttaccgcc agcatcaacg atggtatcta ccgccgaggt
     1381 ttgagctacg accgtcagtt cgccggtatt ttccagcaca acatgctcag ccctgcctcc
     1441 ggtaatagtg aagtgagatg acctctggta tccgtcaata tcagtccccg cgcccgtgtt
     1501 ggcaactaaa gcactccccg tgtccaggta aaccccatgt gcgataccgc cggtatcgac
     1561 aatcagcgtg cctccggcgc gaatattgct gccagttgcc gtacctcctt tctcaacaac
     1621 ctgcttgctc ccggaagaga tatttgttgt gtcagctttc ccgccgctct tgatattttg
     1681 cgtgccactg ttgatgttgg ttcccgtagc aataccatga ttataaatat tctgtgtgcc
     1741 gccattaatt atggtattag tcgcgttccc ggcaacatcc atggccccgc cgttatctat
     1801 tcgggtcgca tcagctttag cattcgttaa aactgacatt gttcctttat cgttaattat
     1861 cgtccctgta gaaattcctc cctcactaat tgtctggctg ccaccgttaa tgactgtatt
     1921 attagcctgc ccctgataag agccatgacc agatacctct tgatgtccac cagtatcaat
     1981 aacacttcca ttagaaatcc cgccatatac ctcctgattg ccgtgattga tgatatgagc
     2041 attatttgtt gtacctcgtt catctactct ttggctgccg tctacagtct catcatttac
     2101 aacgccaata acattagaag tgaaggccgt catcccaggc ggggcatata tcatggcagc
     2161 tatcaataag gaaagtactg agcggtgaaa ataatagcga ctgattctgt tcataaattt
     2221 catcctctga aaaatgaata ctgagtagcg tctaagtaac ctaagctcgg ctgcaacatc
     2281 agcccgcagg caccagacca ggggattcat cctgaagaga cagcgcaggt gtattgtgtt
     2341 cgccgctcac caaagacatc atgatgaaat gatgatatcc cgcataagaa agaggcattt
     2401 tttaaacgca gtgcgctgaa gtgtagttgg ataaaaaatc actcttttca gataatactg
     2461 tcgtacgctt atctttcgac aatgaggccg tcggcaaaat aaaatgacta a
//
LOCUS       DAZGBC010000333         2491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGBC010000333 DAZGBC010000000
VERSION     DAZGBC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.335"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(3..305)
                     /locus_tag="ACHK1J_005119"
     CDS             complement(3..305)
                     /locus_tag="ACHK1J_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914245.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(302..928)
                     /locus_tag="ACHK1J_005120"
     CDS             complement(302..928)
                     /locus_tag="ACHK1J_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFO7914246.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            complement(1140..2411)
                     /locus_tag="ACHK1J_005121"
     CDS             complement(1140..2411)
                     /locus_tag="ACHK1J_005121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO7914247.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVESYSIDE
                     MFIDLREVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPEKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(2411..>2491)
                     /locus_tag="ACHK1J_005122"
     CDS             complement(2411..>2491)
                     /locus_tag="ACHK1J_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO7914248.1"
                     /translation="FRTLYPENICIFGVVTHVIHRTRELR"
BASE COUNT          621 a          622 c          522 g          726 t
ORIGIN      
        1 tgttatcttt gttttacttc tgctatcaat cgtgatacct ctgtttttga tagtgtttta
       61 agtgcttcta ccgccgccct gacaacatca gaacgattca ctctgccttt acctgaaacc
      121 atctcagccg ttataagggc atcaatgtct tttaggtttt cttcaaaaaa agaaatactc
      181 actggttttg ctttgcttac aaccgatgat ttatctgatg actttttggt attatcaagg
      241 ccagccgtgc caccgttaat aaatgctgcc tcgtcacgag gtgaagtgtt tgctctattt
      301 ctcacagaat aacctctttc actaacgctt cgatttcttg ctgggcttta tcattttgcg
      361 tttcaaaaat tgtcattgat tcggcccaag catctcgatg tgctttacga tcacataccc
      421 gggagtttgc cagatgcatt tcagggtaat ccttaagtac ttcagctgcc tcattggctt
      481 cattaacaaa catatttgtg ggcgtcatgt ttaaaacgag atatccccga acatcctcat
      541 taaaatcttt tgctgctgta aaaacggagc atgtatgagg cacaacatcc aggtccatct
      601 gagatgggcg caaaggagaa atgaatacat ctgcggccat caatccacta cgcatttctg
      661 cgctatcacg gccagcacag tctgcaataa caaaatcata ggatttttcg tgttccttta
      721 acatggcttt gatgttacct gtcgcgcctg taaccggaat atgagggaga tcttcagggc
      781 gattgttgta ccaggttaat actgatttct ggtcatctgc gtcgacaata atcactctat
      841 tgcctagtga catcagatac gcagctatag ataccgccag tgtactttta cctaccccac
      901 ccttttgact acctaatact attatcatta tagccccata ttaagttgtt aagttaacaa
      961 cttaatattg ccatctgaac gcgaatcatg caattatttt tgttatatta acaacttaat
     1021 aagttgttaa gtatttgatt tatagattaa ttaagatagt gagataacaa gataacaaga
     1081 taacaagata acaagataac aagatagcaa gataacaata ttatgggtgg attacatatt
     1141 cataactggg ctactggcaa atctgtccat ctggtggtat acgcaggaga aagcatttcc
     1201 cgtttcattt gccactcagg ggcggttcct cgccccgcaa accacacttt cccttttcca
     1261 gactggttca gttcatcgag tgttttcatt aacttttcgc tgtttttacg gggctggatc
     1321 tcatcaaata atcccggctg cgctataccc gatggtgtga aatcagccag catgactcct
     1381 gccttcatat agcggtaccc ttcacgccag acatggttta acgctctgca tgctgcggca
     1441 ataatgtccc ggctgtcctg tgtgggcaat ggaagctttt ccacagcggc attgctgtaa
     1501 cagggttctt ttactgcaaa gggggatgtc cgtacaaatg tcgtcacctg ccggcaatac
     1561 tgacgctccc cacgtagttt ctctgcagcc cgctctgcat actgaacaac agcctggtgc
     1621 atggcatctt tgtctgtgat tcgttcacca aaactgcgac tacagacaat ctgctgtttt
     1681 tccggtggtg cttcttccag ggatatgcag gactcgccgt tgagttcgcg taccgtacgc
     1741 tcaagaatga cgctgaagtt tttccggatg aatgccgtgt tagcctgcgc cagctgcagt
     1801 gctgtgttaa tacccagcgc attcagcttt tccgtcagtc tgcgtcctac tccccagacc
     1861 tcaccaactg gctgcagccc cagtagcttc aagatccgat tacggttttc tgccgtcagc
     1921 gcgaccacac cggaaaactg tggccattgc tttgttgccc actgtgcact tttagccagc
     1981 gtttttgtag gtgcaatgcc cacccccatg gtgagtcctg tccagctctt tacctgttcc
     2041 ctgagctgat gaccaaaaac ctccggagag atgcaacggt tcacctcccg caaatcaatg
     2101 aacatttcat caattgagta ggactcaact gcgggagaaa gcgactccag aacagccata
     2161 acccgttggc tcatgctgtg gtacagcgca taattgctgg aaaatacatg tattttcttc
     2221 tccaggcgca tctgtctcac ctgaaaccag ggctgcccca ttctgatgcc aagggctttt
     2281 gcctccgggc tgcgcgcgat cacacagcca tcgttattgc tgagtacgat gaccggttcg
     2341 ttgcgaaggt ccgggcggaa aactttttca catgaggcgt agaaactgtt gatatcagcc
     2401 agtgcaaaca tcagcgtaac tcccgcgtcc tgtgtatcac gtgagtgaca acaccaaaaa
     2461 tacagatgtt ttccggatac agtgtgcgga a
//
LOCUS       DAZGBC010000334         2460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGBC010000334 DAZGBC010000000
VERSION     DAZGBC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.336"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1330
                     /locus_tag="ACHK1J_005123"
     CDS             <1..1330
                     /locus_tag="ACHK1J_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914249.1"
                     /translation="GLDENDKAIYVTDDYLSKVNIKILGATQTGKGVIQGVIADQAIL
                     KNWGLWFWDQKPDKFIYSIMKQRCESEGREIYEVDLNGEGIGDYAPFKNGTKREILDR
                     FFNAFSLETGGTDADYYKNNAQEVMYYIYTFWDRSLNDLQKLLSGSDDRIPEEKQEWI
                     AKNGANIRTQLNKWLELPCLSPADGQGLNVTELVKSGAVIYVKGSTKDKVVRAVLKSM
                     LREWSAAVIREQPKNHVLGIIDEARFVISSEVADSLATILSSNASLSIAYQERDDLLN
                     IPDEKPQIAQSIKKGTETNTNLTLVYRCNDETAEWVAKNSGTTAKSLTKLEEVETDGY
                     GAETWKGRRMIGQEEDYYIPVNTVLAVEERVGIMQTMFELSRVLFTCWVPVQRFYTLP
                     ARPQTEQDNVQDCIKNDGSISPSDEDYFNPDMISDEELAMINKTVSSDSK"
     gene            1387..2451
                     /locus_tag="ACHK1J_005124"
     CDS             1387..2451
                     /locus_tag="ACHK1J_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO7914250.1"
                     /translation="MDKKTKTYPFGEIEFFDQLEEHAWDQIHDGMLPIMDLYLDPTVT
                     DIFINRYDNIYTSVNGKYVKTDSSFSSERALKAWIDQIATVLRQPFSTSGNYKYGDVI
                     EPVLDARFPDGSRLMCTDPVISPQGSTVSLRKVPKQILEEKDFINSGMLTRDILDYLI
                     SVIQKRKNFIVAGNTGSGKTSLLRLLARYIDTMERVITAEDTQELHIHRYFPLGIALE
                     AAHRKDVNADLPSLVHLTMRGNPDRVWVGEVRTAEAIAAYYMVLASGTIGNATTLHSV
                     TPMGAIRKMTWLMSSYLSIDFQIAQDLIMSEVDVIIQCRRTSKHGRRITDIVEIREGK
                     IVPIFKFDEEQEVHVRFDKK"
BASE COUNT          775 a          388 c          578 g          719 t
ORIGIN      
        1 aggattagat gaaaatgata aagcaatcta cgttacagat gactatttgt ctaaagtaaa
       61 tattaaaatt ttaggcgcaa cgcaaacggg taaaggtgtt attcaggggg ttattgcaga
      121 ccaggcaata ttgaaaaatt gggggttgtg gttctgggat cagaaaccag ataaatttat
      181 ttactcgatt atgaaacaac gttgtgagtc agaggggcga gaaatatatg aagttgattt
      241 gaatggtgaa ggtataggtg attatgcgcc atttaagaat ggtacaaaac gtgaaatttt
      301 ggataggttt tttaatgcgt tttctcttga gactggcggg actgatgcag actattacaa
      361 aaataatgca caggaggtta tgtactatat atatactttt tgggaccgtt cgttaaacga
      421 tttacaaaaa ttgttgtctg gtagtgatga tagaataccg gaggaaaaac aggaatggat
      481 agctaaaaat ggagctaata ttcgaacgca gcttaataaa tggttagagc ttccttgtct
      541 atctcccgct gatggtcagg gattaaatgt aactgaactg gttaagtcag gtgcggttat
      601 ttatgtaaaa ggtagcacaa aagataaggt tgtcagggct gtcttaaaat caatgttgcg
      661 tgagtggtct gcggctgtta ttcgtgaaca accaaaaaat catgtgctgg ggataattga
      721 tgaggcccgt tttgttatct cgtcagaggt tgctgatagt ctggcaacta tattatcctc
      781 aaatgcaagt ttgtctattg catatcaaga gagagatgac cttttaaata taccggatga
      841 aaaaccgcag attgctcagt caataaaaaa agggacggaa acaaatacca atttgacact
      901 tgtttatcgt tgtaatgacg agacggctga atgggttgcc aaaaattctg gtacaactgc
      961 gaagagtctg acaaaacttg aagaagtcga aactgacggt tatggcgcgg aaacatggaa
     1021 gggacgacga atgattggac aggaagagga ttactatata ccagtaaata cggttcttgc
     1081 agttgaagaa cgagttggta ttatgcagac tatgtttgaa ctcagtcggg ttttatttac
     1141 atgctgggtt cctgttcaac gtttttatac acttccggcc cgccctcaaa cggagcagga
     1201 taatgttcag gattgtataa agaatgatgg ttcaatttct ccaagtgatg aggattactt
     1261 taatcctgac atgattagtg atgaagagct agcgatgata aataaaacag tatcgtcaga
     1321 cagtaaataa aataaatctg gcatctagct gatgccagat tttttagtta ataatatggt
     1381 gtacatatgg ataaaaagac aaaaacatat ccttttggtg aaattgagtt ttttgaccag
     1441 ttagaggaac atgcatggga ccagattcat gatggtatgc taccaattat ggatttgtat
     1501 cttgatccga cagtaaccga tatatttata aacaggtatg acaatatata tacatctgtc
     1561 aatggcaagt atgtaaagac cgacagttcc ttttccagtg aacgtgcact taaagcctgg
     1621 attgatcaaa tcgcaacggt gttgaggcag ccattttcaa cgtcaggaaa ctataaatat
     1681 ggtgatgtca ttgagcctgt acttgatgcc cgatttcccg atggttctcg gttgatgtgt
     1741 acagacccgg tgatttcacc gcagggttct acggtaagtc ttcgtaaagt acctaagcaa
     1801 atactggaag aaaaagattt tataaattcc ggtatgttga cgagagatat tcttgattat
     1861 ctcattagtg ttatacaaaa acggaaaaat tttattgttg ctggtaatac tggttctggc
     1921 aaaacatcac ttttacgctt gctcgccagg tatattgata cgatggagcg tgttatcacc
     1981 gccgaagaca ctcaagaact tcatatacac aggtattttc cgttagggat tgctctggaa
     2041 gctgcacatc gtaaagatgt gaatgcggat cttccttctc tggttcacct gactatgcgt
     2101 ggtaatcccg atcgtgtatg ggttggggag gtaagaacgg cagaggctat agctgcttat
     2161 tacatggttc tggcaagtgg aactataggt aatgctacaa ctctgcactc cgttacccct
     2221 atgggggcaa tacgcaaaat gacctggctt atgtcaagct atctcagtat cgattttcag
     2281 atagctcagg atttaattat gtcagaggtt gacgtgatta ttcagtgccg gagaacatca
     2341 aagcatggaa gaagaattac agatatcgtc gaaatacgtg aagggaaaat tgttcctata
     2401 ttcaagtttg atgaagagca agaagtacat gtcaggtttg acaaaaaatg aacatttagc
//
LOCUS       DAZGBC010000335         2434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGBC010000335 DAZGBC010000000
VERSION     DAZGBC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.337"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..457
                     /locus_tag="ACHK1J_005125"
     CDS             <1..457
                     /locus_tag="ACHK1J_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914251.1"
                     /translation="EVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEGGLYNLRGL
                     DFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTPCIFNTPANHESYEKSLKPVSENGLNG
                     VLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPEDAPIENSPIVNSPLV"
     gene            826..984
                     /locus_tag="ACHK1J_005126"
                     /pseudo
     CDS             826..984
                     /locus_tag="ACHK1J_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1208..1327
                     /locus_tag="ACHK1J_005127"
     CDS             1208..1327
                     /locus_tag="ACHK1J_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO7914252.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            1392..>2434
                     /locus_tag="ACHK1J_005128"
     CDS             1392..>2434
                     /locus_tag="ACHK1J_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO7914253.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPGMAAFTSDVIGVVNDETV
                     DGTQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTVKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSTSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLT"
BASE COUNT          740 a          450 c          577 g          667 t
ORIGIN      
        1 agaagtcaag ctgtctggag aacatcttga aacggtattc aaatacaaaa atggcgatga
       61 tgtccgggag atagccaaaa ttaacattac tctccaaaaa gaagagggtg gcttatataa
      121 tctacgtgga ttggatttta aggggtgctt cttttctgga cagaacttca gtaactatga
      181 tattcaatat gtgaactggg gaacgtcatt gtttgatgtt gatactccgt gtatttttaa
      241 tacgcctgct aaccatgaga gttatgaaaa atcattaaaa cctgtaagcg aaaacggttt
      301 aaatggagtc ttatctgatc gtaataaaaa aataaaaatg atcacgggtg tggcaccatt
      361 cgatgatatt ttatttatgg atgatgactt tgatgataac tcccctgagg atgctcccat
      421 tgagaatagt cctattgtga atagtcccct tgtatgaagt tctaaaagta gttttcaatg
      481 ataaataaat ggctattttt acgaagagat aataacgtag attgaaacaa tatgtattat
      541 tgattgagta aatgggatta ttcttcacac tacatccatt ttagtgtcac taccaaagaa
      601 aaaggggcta cgttttcatg taaccccttg atttatttgg tgggctagca gtgactgaat
      661 tggtgctgta attggttgaa atatttcatc taatgtctgg ttcaacttcc ttagctgcct
      721 gaaaattttt tgagcttacc cagcaataac agcgttacgg tgcaccacgc ctgactgaag
      781 aactgcgtgc tcagggttac cacttcaacg taaaaccgtg gcaggaggtc cggactgaaa
      841 aaactgctca ggacatcatc ggcagggaac gcactgctag tctgtaaact gaaccgactg
      901 gggcgcagta tgtggcatct tgtcgtgctg ttggaggagc tgtgcgaacg tggtattaac
      961 tttcgtgctc tggcccaatc tatatttgcc caacaatggg gggacgaatg ctgtaaaagt
     1021 aaaacaatct gcgatctcaa agttattgtg tgatttttat gtgagcagaa gatattcatc
     1081 agaaacgatt atgtaaatca ttttattttg ccaacggcct cattgtcgaa agaaaagaat
     1141 acgcccgtgt ttcctgaata gattgacttt tttatccaac cacacttcag cgcactgcgt
     1201 ttaaaaaatg cctcattctt atgcggaata tcatcatttc atcatgatgt ctttgatgag
     1261 cggtgaacac aatacacttg cgctgtctct tcaggatgaa tcccctggtc tggtgcctgt
     1321 gggctgatgt tgcagcaaag ctaaggtcgc ttaaacgcta ctcagtattc acttttcaga
     1381 ggatgaaatt tatgaacagg accagtcccc attattgtcg ccgctcagta ctttccttat
     1441 tgatatctgc cttgatatat gccccgcccg ggatggcggc cttcacttct gatgttattg
     1501 gtgtggtaaa cgatgagact gtagatggca cccaaagagt agatgaacga ggtacaacaa
     1561 ataacactca tattatcaac catggccagc agaatgttca tggcggggta tctaatggaa
     1621 gtcttattga atctggtgga tatcaagatg taggaagtca taacaatttt gtggggcagg
     1681 ctaataatac aaccattaac gggggcagac agtcaattca tgacgggggt atttccacag
     1741 gtacgataat cgagagtggc aatcaggacg tttataaagg gggtatcagc aatggaacga
     1801 cagttaaggg cggtgcttca cgcgtagagg gagggagtgc gaatggaaca ctcattgatg
     1861 gtggtagcca gatagtaaaa gttcaagggc atgctgatgg tacaacgata aataagtctg
     1921 gctctcagga cgtagtacaa ggaagtctgg caacgaacac aaccataaat ggtggtcgac
     1981 agtatgttga acagagcaca gtagaaacaa ccaccatcaa aaatggcggt gagcaaagag
     2041 tatatgagag ccgtgcgctg gacacgacga ttgaaggcgg aactcagtct ctgaatagta
     2101 agtcaacggc aaaaaatacg cagatctatt ctggtggcac gcaaattgtt gataacacca
     2161 gcacctcgga tgttattgaa gtttattccg gtggcgtgct tgatgttagt ggtggtacgg
     2221 caacaaatgt tacccagcac gatggtgcaa ttttaaaaac taacactaac ggtacgacgg
     2281 tgagcggtac gaatagtgaa ggtgcattct ccatccacaa tcacgtggca gacaatgtgt
     2341 tgctggaaaa cggtggtcat ttagacataa acgcatatgg ttcggcaaac aagacgatta
     2401 ttaaagataa aggaacaatg tcagttttaa ccaa
//
LOCUS       DAZGBC010000336         2380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGBC010000336 DAZGBC010000000
VERSION     DAZGBC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.338"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..1604
                     /locus_tag="ACHK1J_005129"
     CDS             246..1604
                     /locus_tag="ACHK1J_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7914254.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQMIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVLG
                     GPIVAHILSSHYESYDLHIAELTNENGQVVWKTSYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMLMDIDHFKKVNDTWGHPIG
                     DQVIKTVVNIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1702..>2380
                     /locus_tag="ACHK1J_005130"
     CDS             1702..>2380
                     /locus_tag="ACHK1J_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFO7914255.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNND"
BASE COUNT          698 a          384 c          504 g          794 t
ORIGIN      
        1 caattgttat tcctaatata tctatgaatg aatgttttat aaggatttcc ttataaccat
       61 ttataaatat ttgcaagcct gtcttgtcgc aaagaatgta taaagcacta aggtaataat
      121 aattgcggtc tgcaactatc atcatcggta ttgtttgtga gtgggttttc atattgacaa
      181 ctgtacagtt cttatatcta ttaataatag aaagggatct acaacctaca ggttggtgta
      241 gctttatgga aaaagactat ttgagaatta gtagtactgt cttagtgagc ttattgtttg
      301 ggcttgcttt ggttttggtg aatagttggt ttaatcagcc aggcgttgag gaagttgttc
      361 cacgatcaac ctatctgatg gtaatgattg ctttattttt tatcgatact gttgcattta
      421 tttttatgca gttgtatttc atttatgacc gtaggcaatt ttcaaactgt atacttagtt
      481 tggcttttct gagttgtttg atttactttg ttaaaaccgt cattatcatt cagcaaatga
      541 ttgaggggcg tttgacaagc agtgttgttc aaaatgacat tgcaatttat tatttgtttc
      601 gtcagatgag tttgtgcata ttaatatttc tggcattggt gaataaagtt agtgaaaaca
      661 caaaacagcg caatttattt tccaaaaaaa tgactttgtg cataagtttg ttttttgttc
      721 ttggggggcc gatagttgct cacatacttt ctagccatta tgagagctac gatttacaca
      781 ttgccgagtt aaccaatgaa aatggtcaag tggtttggaa aacttcatat gttaccataa
      841 tgattttcat gtggttaacc ttgctttcgg ttaatctata ctttaatggt ttacgctatg
      901 atatctggaa tggagtgacg gtgattgctt tttgtgccgt actgtacaat gtctctcttt
      961 tatttatgag caggtatagc gtgtccattt ggtatattag tcgcactatt gaagtagtca
     1021 gtaagttaac cgttatggta atatttatgt gccatatatt cagtgcgcta cgagtaacaa
     1081 aggacattgc acatcgcgat cccttaacca atatatttaa cagaaattat ttttttaatg
     1141 aactgacggt tcaatcagca tcggccaaaa aaacgcctta ttgcgtcatg cttatggata
     1201 tcgaccactt caaaaaagtc aacgacacct gggggcatcc gattggcgat caggtgataa
     1261 aaacagtggt gaatatcatt gggaaaagca tacggccaga tgaccttttt gcgcgcgtcg
     1321 gcggcgaaga gtttggcgtc ttgctgacgg acatcgatac tgaacgtgcg aaagctttag
     1381 cggaaaggat tcgggaaaat gttgagcgtt taactggtga taatcctgaa tacgccatac
     1441 cacaaaaagt gacgattagt attggcgctg ttgtgactca gggaaatgcg ttaaacccga
     1501 atgagatcta tcgactggct gataacgcac tatacgaggc gaaagagacc gggcgtaata
     1561 aggtggttgt gagggatgtg gtgaattttt gtgagtcacc ataaagcggc attttagcgg
     1621 gtgattttgg taagcgctaa atagctattc gcagacatgc gtaacaattt tcagaaatat
     1681 catagtacta tatgatctta aatgcttatc attgatcagt ctgctatcac ccgcttttac
     1741 tggcaattgt actataattt cttaggttgc atcaccctga tgcgtgggga aacatgtatg
     1801 gtattcaaca aaaaaatgtt tgtactcatt ataataccag gcattctggg ggtgttactg
     1861 tcgtttgcta tgtcagtatt tcaaatgaat cgagatacga caattaccgc cggtattctg
     1921 ttaaaacagc ttgataatgt tacgcaaatt gtcaaacata cgacaaagct tacttctatt
     1981 ctggtgatga aaccctgcaa agatatcctg gaacaattga ttgctaacgg agcattgacc
     2041 ccctatgttc gaacaacggg tcttatagaa aataatttcc aaatatgttc ttcagttagc
     2101 ggtttcaaaa aaatgaatgt taacgatgtt tacggtacga gctttcacaa taaaaataaa
     2161 gaatcacgca tagtatcaat tagcggtaca agttttgtac caggtaaaac agccatagtg
     2221 ttccttatgc caatagggaa tgatatgaca gcttttagta ttgtagagtc acgatatatc
     2281 tatgatctga tggatgttct tgatgatgag aatgatgatt cattttctct tcgctttact
     2341 gaagggcctg caattattag tggtgtaaat aataatgaca
//
LOCUS       DAZGBC010000337         2371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGBC010000337 DAZGBC010000000
VERSION     DAZGBC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.339"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(25..273)
                     /locus_tag="ACHK1J_005131"
     CDS             complement(25..273)
                     /locus_tag="ACHK1J_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7914256.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERIMSLLEEIWQDDSWLPAA"
     gene            691..1593
                     /locus_tag="ACHK1J_005132"
     CDS             691..1593
                     /locus_tag="ACHK1J_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7914257.1"
                     /translation="MYFSAEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKNMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVVAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            1590..1901
                     /locus_tag="ACHK1J_005133"
     CDS             1590..1901
                     /locus_tag="ACHK1J_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914258.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRSLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRT
                     R"
     gene            1977..>2371
                     /locus_tag="ACHK1J_005134"
     CDS             1977..>2371
                     /locus_tag="ACHK1J_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO7914259.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVVGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLP"
BASE COUNT          476 a          642 c          690 g          563 t
ORIGIN      
        1 cagccggacg gtgataaacg gtactcatgc tgcaggcagc cagctgtcgt cctgccagat
       61 ttcttccagc agagacatga tacgttcttt atccttgtcg ttgcgggctc ctgtgacaga
      121 tacagacggg gcgctgccct ggtctatacg cagccagatt tccgggtagt gtggtttcag
      181 acgttgttcg atttcattct gtagcgcctg aagtgtgcct gatttgaggt tttttgtgct
      241 ctggcggtcg aaaagaattt caatgcggat catgttctca tctccctttt ctgttgctgt
      301 atgcatatac agtattctaa taactgaatc cacacacagt caaatgtcat tattgtgact
      361 aaaaaacgct caattctctg agggggtggc tgtatgcctg ctgtggactg ctgtgtccag
      421 cttatccaca gcattttgcg cacggttctg tgaataaaat acctggttac ccaggccgtg
      481 ccggcacgtt aaccgggctg catccgatgc aagtgtgtcg ctgtcgccgg cctcctcacc
      541 cggtcacgtt tcgtcgttcc tcctccacgc gctgcggctt cggggccgca cctgcattcg
      601 tatgcggtcg cccggttaca ggtgcggcac ggcctgatgg aggccgcatg tgagaggaga
      661 attcccatgc caaactggtg cagtaaccgt atgtattttt ctgccgaacc ggcacagatc
      721 gctgagatta aacgactggc cagcggcgca gtcacaccgt tttatcgccg cgccacaaat
      781 gaaggtattc agctgtttct ggccggaagt gccggacttc tgcagaccac tgaagatgtg
      841 cagtttgaac cgtgccccgg actgacggct gccggacgtg gtgttgtatc gccggagaat
      901 atcgcgttca cacgctggct gacacacctg cagaacggtg tgctactgga tgaacaaaac
      961 tgcctgatgc tgcatgaact ctggctgcag agcggtactg gacagcgtcg ctgggaagga
     1021 ttaccggacg atgtcaggga taccatcacc gcacttttca ccgcaaaaag aggtgactgg
     1081 tgtggcttct ggagtaacga ggatgtatcg gtgtggtgga accgtctgtg tgacaacgta
     1141 ctgccggaaa aaaacatgcc gtttgacctg ctgacggttc tgccgacccg cctggatgtt
     1201 gaagtgaatg gctttaacgg cggtgttctg aacggtgttc cttctgcata tcactggtat
     1261 acagaacggt atggcgtgaa gtggccttgt gggtatgacc tgaatatcag cagtcaggga
     1321 gacaacttca ttcaggtgga tttcgacacg ccgtggtgtc agccggaaag cgacgttgtt
     1381 gcagaattaa gccgccgttt cagctgcacg ctggagcact ggtatgccga acagggctgt
     1441 gatttctgtg gctggcagtt gtatgagcgc ggagagctcg ttgatgtgct gtggggggaa
     1501 ctggaatggt cttccccgac agatgacgat gagctgccgg aagtcaccgg acctgcgtgg
     1561 atagtcgaca atgtggcgca ttatggcgga tgaagtatga cggagacggg cgggcaaccg
     1621 cccgtttctt ttccggtaaa ggatgtcgcc gtgctcctct ttttactgcg ccggctgacg
     1681 cggcgcggca taaacgcagc ctgtggtcag tgtcccgcgt ccggcgggca cgggacgggc
     1741 gcttttaccg ctcccggctg gcgtcggtga cagtgtacgc cagcctgtcg cccttttctg
     1801 atgaacgccc ttcaagccgc tttcgcggca taaccttgcc gtcaaacaga cgacggctgc
     1861 ggtattccac ggtcggcctg acccgttacc ggacgcggtg aacagcccac aggcagcggg
     1921 gaacgggcac cacaggggtg ccctcccggt gcccactgta agagaaggag ttttgtatgt
     1981 cccgttttgt cctcggtaac tgcatcgatg ttatggcccg tatccctgat aacgccattg
     2041 atttcatcct caccgacccg ccgtacctcg tcggtttccg tgaccgttcc gggcgcacca
     2101 tcgctggcga taaaaccgat gaatggctgc aaccggcctg taatgaaatg taccgcgtac
     2161 tcaaaaaaga cgcgctgatg gtcagcttct acggctggca ccgtattgac cgctttatgg
     2221 cggcctggaa acgcgccgga ttcagcgtgg ttggccacct ggtcttcacc aaaaactaca
     2281 catccaaatc tgcgtatgtc gcgtaccgcc acgaatgcgc ttacatcctg gcaaagggca
     2341 gtccggcccg gccccgtaac ccgctgccgg a
//
LOCUS       DAZGBC010000338         2330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGBC010000338 DAZGBC010000000
VERSION     DAZGBC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.340"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            344..907
                     /locus_tag="ACHK1J_005135"
     CDS             344..907
                     /locus_tag="ACHK1J_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7914260.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            904..>2330
                     /locus_tag="ACHK1J_005136"
     CDS             904..>2330
                     /locus_tag="ACHK1J_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7914261.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLLYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGA"
BASE COUNT          523 a          561 c          754 g          492 t
ORIGIN      
        1 ctgccggagg tgtccggagg tagtggcaga gcggacgaca tcatcggact gtaacccgac
       61 cggggggggt atcatccggc gtaaaaaacg atcgctccgg acaccgcgcc ccctctcacg
      121 cagagaaaaa attcccgttt cagggcagtt aacatgttaa ctggctgtcc gggcattttt
      181 gcggttttta tctttattat tcagtttgtt gcgtggaaaa aatgttaaca ggctttttca
      241 gcaaatgtta accaggcagc agttaacatt tgcggcatga gacgccggga aaaatgggct
      301 gaaccatacc cggctgagtg cgttctggac ccgggaggag gctgtgctga caacgcaaaa
      361 acgaaaattt gcgctggcgc tcatgtccgg gaaaaacaaa acagcgtcag ccattgccgc
      421 cggttattcg gcgaagacag ccagggttaa aggctcgcag ctggcaaaag atccggaggt
      481 gcttgcgttt atagcccgta aacagtgcga gacggtggag gtggatgagg ttcctgttta
      541 ccggcagaaa aaatcagagc aggaggataa accccgtcgc cgtgaggcgg ctgcaatacc
      601 acagccggac gaaacaaatc cggagatgcc accgcccgtg gtgatatctc ctggtattga
      661 gtatatggag gacggtcttc ccgatccggt gaaagcgatg gggcgtcttc tggtggagaa
      721 cattaatacc gaccccaggc tggcgctgga tgcggcttat aagctggcgc agttcacgca
      781 ccacaaaaaa ggggatgccg gtaaaaaatc ggcaaaaggt gacgcggcga aaaaagcggc
      841 taaccgtttt gcggtgccac caccaccccg actggtggtg aataatgata atgagggcaa
      901 cggatgatac ctgtgtggag cacggcctgc ccggactggg cagagcgcct gaaaaagggg
      961 ctgtcgatta ttccggcccc gatttatccg gatcaggctg cacatgcact ggcgattttt
     1021 aaacaactgc ggattgtgga tgcaccgggc agcccgacgt tcggggagtc ctgtgcaccg
     1081 tgggtgtttg acctggtggc ggccctgttt ggctcctacg atgcgcagac cggtgtacgc
     1141 catatcaagg aagtttttat ccttatcccc aagaaaaaca gcaagtccac gctggctgcc
     1201 gggatcatga tgacggcgct gttactgaac tggcggcagg cggcgggcta caccattctg
     1261 gccccgaccg tggaggtggc ggctaacgcc tttaatcctg cgcgggatat ggtacgacgg
     1321 gacgatgatc tggatgacct ctgtcaggtg cagacacata tccggaccat cacccatcgg
     1381 gtgacggaca ccacccttaa ggtggtggct gccgatccga atacggtatc cggtatcaag
     1441 tccgtgggga cgctgattga tgaactgtgg ttatttggca agcagtacaa agcggaggac
     1501 atgttacgtg aagccatagg tggccttgcc tcccgcccgg aagggtttgt ggtgtatacg
     1561 accacccagt cgaatgaacc gcccgccggg gtgttcagac agaaactgct gtacgcccgg
     1621 gatgtccgtg acggcaaaat tcatgatccg cactttctgc cggtgatatt tgaacaccct
     1681 cctgaaatgg tggaaagcgg ggctcacctg ctgatggaaa acctcgccat ggtcaatccg
     1741 aatctcggtt attcggtgga tgaggctttt ctgtaccggg agtaccgtaa agcccgggag
     1801 gctggtgagg aagcatttcg tggcttcatg tcaaaacatg ccaatgtgga aattggtctt
     1861 gccctgcgtt ctgaccgctg ggcgggtgcg gatttctggg agcagcaggg caggcgcgtc
     1921 agcctggacg atatcctgca gcgcgctgat gtggtgacgg tggggattga cggcgggggc
     1981 ctggatgatc tgctgggaat gtacgtgatt ggccgtgaca gggaaacccg cgaatggctg
     2041 ggctggggcc atgcctgggc gcatgaaacc gcggtggtca gacggaagag tgaggcatcc
     2101 cggtttcagg attttgtggc ctgtggagat atgacgattg tccgtcgggt cggggatgac
     2161 acggcggaag tggcggagta tgtgcgtcgt attcatgagg ctgagttact ggagcatatc
     2221 ggtattgacc cgtcaggtgt ggggcagatt ctggattcac tggtggaagc cgggatcccc
     2281 gacggaattg ttgtggggat aagccagggc tggaagctgg gcggggccat
//
LOCUS       DAZGBC010000339         2324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGBC010000339 DAZGBC010000000
VERSION     DAZGBC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.341"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1077
                     /locus_tag="ACHK1J_005137"
     CDS             <1..1077
                     /locus_tag="ACHK1J_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7914262.1"
                     /translation="FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRV
                     RVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIM
                     GRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTE
                     VLNNRTTDVINNHAETIGNNQIIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIG
                     LVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKT
                     KKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINW
                     NCAASKSPPKTPDEKQDTPDMREY"
     gene            1144..>2324
                     /gene="rhsD"
                     /locus_tag="ACHK1J_005138"
     CDS             1144..>2324
                     /gene="rhsD"
                     /locus_tag="ACHK1J_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO7914263.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKATQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGGITGVTDDAGREFRLVLTTQAQRAEEARTSSLSSSDRSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VT"
BASE COUNT          514 a          636 c          747 g          427 t
ORIGIN      
        1 tttgcggtaa taccggcaga cagaacatgg cgaccacagc cgttgctgaa accgctggtg
       61 gacggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt ctgtgatgag
      121 catggccgcg tgcgggtgaa atttaactgg gaccgttata acccgtcaaa ccaggacagt
      181 tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gattcggtaa ccttgcgata
      241 ccgcgcgtgg gtcaggaggt gattgtggac ttcctcaacg gcgatccgga ccagccgatc
      301 attatggggc gcacctacca ccaggaaaac cgcacacccg gcagcctgcc gggaacaaag
      361 acgcagatga ccattcgttc gaaaacctat aagggcagcg ggtttaatga actgaagttt
      421 gacgatgcga cagggaaaga acaggtctac atccacgcgc agaagaacat gaacaccgag
      481 gtgctgaata accgcaccac ggatgtgata aataaccatg ctgagaccat cggcaacaat
      541 cagataattg cggttaccaa caatcagata cagacggtgg gcgttaacca gatagagacg
      601 gtgggcagta accagatcat taacgtgggt tctgttcagg tggaaacgat tggacttgtt
      661 cgtgcgctga ccgtgggcgt ggcgtatcag acgacggtag gtggcattat gaacacctcg
      721 gtggctctga tgcagtcctc gcagatcggt ttgcataaat cgttgagggt cgggctgggt
      781 tatgacgtca aagtcggaaa taacgttacc ttcaccgttg gtaaaacgaa aaaggatgat
      841 accgggcaga ccgcgattta ctccgccggt gagcatctgg agctctgctg tggtaaggca
      901 aggctggtgc tgacgaagga cggacaaatt tttctcaacg gcacaaaaat tcatttgcag
      961 ggtaaggagc aggttaatgg tgactcactg ttgattaact ggaactgtgc agcctcgaaa
     1021 tccccaccga agacccctga tgaaaagcag gatacgccgg atatgagaga gtactgatcc
     1081 cgtacctgtt cggtattcag taacaaacaa gcattaaaaa taaacagaag aaggaattga
     1141 atcatgagcg gaaaaccagc ggcgcgtcag ggagatatga cccagtatgg cggtcccatt
     1201 gtccagggtt cggcaggtgt aagaattggc gcgccaactg gcgtggcgtg ctcggtgtgt
     1261 ccgggcggga tgacttcggg caacccggta aatccgctgc tgggggcgaa ggtgctgccc
     1321 ggcgagacgg accttgcgct gcccggcccg ctgccgttca ttctctcccg cacctacagc
     1381 agctaccgga cccggacgcc tgcaccggtg ggcgttttcg gccccggctg gaaagcgcct
     1441 tctgatatcc gcttacagct acgtgatgac ggactgatac tcaacgacaa cggcgggcgg
     1501 agcattcact ttgagccgct gctgccgggg gaggcggtgt acagccgcag cgagtcaatg
     1561 tggctggtgc gcggtggtaa ggcaacgcag ccggacggcc atacgctggc acggctgtgg
     1621 gcgtcgctgc cgccggatat ccggttaagc ccgcatcttt acctggcgac caacagcgca
     1681 caggggccgt ggtggatact ggggtggtct gagcgggtgc cgggtgctga ggacgtactg
     1741 ccagcgccgc tgccgccgta ccgggtgctt accgggatgg cggaccgctt cgggcggacg
     1801 ctgacgtacc ggcgtgaggc cgccggtgac ctggccgggg gaatcaccgg cgtgacggac
     1861 gatgccgggc gggagttccg tctggtgctg accacgcagg cgcagcgtgc ggaagaggcc
     1921 cgcacctctt cgctatcttc ttctgaccgt tcccgccctc tctcagcctc agcgttcccc
     1981 gacacactgc ccggtaccga atacggcccc gacaggggca tccgcctttc ggcggtgtgg
     2041 ctgatgcacg acccggcata cccggagagc ctgcccgctg cgccactggt gcggtacacg
     2101 tatacggaag ccggtgaact gctggcggta tatgaccgca gcaatacgca ggtgcgcgct
     2161 ttcacatatg acgcgcagca ccccgggcgg atggtggcac accgttacgc gggaaggccg
     2221 gagatgcgct accgctacga cgatgcgggg cgggtggtgg agcagcttaa cccggcaggc
     2281 ctgagttacc gctaccagta tgagcaggac cgcatcaccg tcac
//
LOCUS       DAZGBC010000340         2300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGBC010000340 DAZGBC010000000
VERSION     DAZGBC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.342"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            700..1869
                     /locus_tag="ACHK1J_005139"
     CDS             700..1869
                     /locus_tag="ACHK1J_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7914264.1"
                     /translation="MFQKNKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISNGRDRRLTSGEERRISRYFRDKNPQLYVIFHLALETAMRQGEI
                     LSLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLR
                     AYHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISH
                     ASVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            1911..2201
                     /locus_tag="ACHK1J_005140"
     CDS             1911..2201
                     /locus_tag="ACHK1J_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914265.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT          640 a          617 c          473 g          570 t
ORIGIN      
        1 cggtatgcct tactgggccc agggaagcac aacggaaatg cgccccaaaa acattgcctt
       61 taactacatc gtcaaagcaa gctgatgcat ttaaggcgta ttaactttta acttacagca
      121 acgtattttg acccagacca tgcttcccga ataccccggt ttacctctgt acgctctgaa
      181 tcctgccgca ccatagcctg ctttgccgtc acagacggca aagccgtttg agccactacc
      241 ccagttcgca taaaccggtt ttctggagtc ttcttctgcg tcataacagg ccgaataatc
      301 aaaaaccagt gcatcgctga tattattctt ccagacaccg gattggcacg ctggctactg
      361 cctgacgctt aattaaatac cggccccagg tatattgaga cacgtaacag atatcgttcc
      421 acctgactca taattacccc acgcataatt attgacatac cagacattat ttttatcgga
      481 ataaacctgc tgaaaaccac cgaaaggaga accattaaga tacgtccatg cacaatatgc
      541 atggacacca agtctgaaat tacgaatatt ttttccgatg ttataggtct gcgttgtgaa
      601 tttaatttca gttgaccgcc agacaccgga ttggcacgaa agtattgcat ttctgtatcc
      661 agcctcctac cttccccgtt tttatcgtgg aaggcagata tgtttcaaaa aaacaaaact
      721 cgcaaaatgt ccctcagccg ggcactggac aaatacctga agactgtttc cgtccataaa
      781 aaaggtcatc tgcaggagtt ttatcgcgtc aatgtgatta aacgtcaccc tatggccgat
      841 cgctatatgg atgaaatcac taccattgat atcgccggtt accgggatca gcgtttagct
      901 caaattaacc cacgaacagg gcgtcaaatc accggaaata ccgtccgtct tgaacttgcc
      961 cttttatcat cattattcaa tatcgcccgt gtggaatggg gaacatgccg tatgaatcct
     1021 gttgaactgg tccgaaaacc gaaaatcagt aatggccgcg accgccgact gacttctggt
     1081 gaagaacgcc gcatttcccg ctatttcagg gacaaaaatc cgcagctcta cgttatcttc
     1141 catctggctc tggaaacagc aatgcgccag ggagaaattc tctcattacg ctgggaacac
     1201 ctcgatttgc agcacggcgt ggcgcatctt cctgaaacga aaaatggctc accacgcgac
     1261 gtaccactct cccggaaagc acgtaactac ctgcaaattc tgccacaaca gataaacggc
     1321 aatgtgttca gttacacctc gtccggtttc aaaagcgcct ggcgtaccgc tcttctggat
     1381 ctgaaaatag aaaatctgca ttttcacgat ctccgccatg aggccatcag ccgtctgttc
     1441 gagctcggca cactgaacgt tatggagatt gcagccattt ccggtcaccg ctccctgaac
     1501 atgttgaaac ggtacacaca tctccgggca tatcacctgg tcagtaaact cgatgcaagg
     1561 cgtaaacaga ccagcaaaat ttccccttat tttgttccct accctgcaac tgtcagaaac
     1621 agaaacggag tgttcattgt gacactgcac gatttcgacc ttgaaagccg tgctgaaacc
     1681 cgggaactgg caatatctca cgccagcgta ttgttgctgc gtgcactggc ccaggctgct
     1741 cagcggggtg aacgcgtccc cacaccaggc gagctacccg caaacattga tgaacgggtc
     1801 atgatttgtc cgttaacaaa cagccagttc atgacaaaag cgaacagtat tttcatatcc
     1861 tcctcctgaa caaataattt ttgttcaaaa ttaacgctat gagacccttt atgaaacact
     1921 tatcctcccg tgccgccact gcggataata atcgtatcgt tacaattgaa gacgtttaca
     1981 caatcgcatt ctcgcaacca attcacaccg aatcagcgtt ggggcaaagg tggatgttga
     2041 tccatactgg tgaatatgaa agtgccagcg atgcaaaacg cttccttgat acgctcgtca
     2101 ccgggcactg tgcttgcatg gtaacaccat cacctgttct ggatcatgac acctggctgc
     2161 gtatccgcct gctgatgggc gagataaggg atgccaaata aacattcatg gccccaacgc
     2221 ataaaaataa cgccggggac tctggcagag gtaccattct ttttacatcg agccaatata
     2281 taaacaactt ttctatgata
//
LOCUS       DAZGBC010000341         2299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGBC010000341 DAZGBC010000000
VERSION     DAZGBC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.343"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..153
                     /locus_tag="ACHK1J_005141"
     CDS             <1..153
                     /locus_tag="ACHK1J_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO7914266.1"
                     /translation="RLTPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTF
                     LGSTNF"
     gene            167..1588
                     /locus_tag="ACHK1J_005142"
     CDS             167..1588
                     /locus_tag="ACHK1J_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO7914267.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            1593..1934
                     /locus_tag="ACHK1J_005143"
     CDS             1593..1934
                     /locus_tag="ACHK1J_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914268.1"
                     /translation="MSRIYGRVKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            1941..>2299
                     /locus_tag="ACHK1J_005144"
     CDS             1941..>2299
                     /locus_tag="ACHK1J_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO7914269.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVIS"
BASE COUNT          531 a          497 c          769 g          502 t
ORIGIN      
        1 agactgacgc cttcagtctc cacgtcctgc gcagtcttcc ctgacggaga gcatgaagcg
       61 gctgaaacag gtaatgctgc ctttgccctg tggcagccct acagttgctg caagaaacgg
      121 ggacagacat tcctgggcag tacgaatttt taagtaaggg gaagtgatga aacagaaaat
      181 gacgatacct gttctggctg tggtgctttg tgcgggtacg gtacaggctg acggaaactt
      241 tttgtccctg ccgaaagtga atgacagtgc gtttggctac ggtaaatccg tggacggcgc
      301 agtatcagac aaactgtttt ataccctggg agggggctcg gtgatatcgc agccggcaac
      361 gcgcagcaat atgcagaaac ttggggtgga tctcgggtgg agcagtgatc tgatgtgtgg
      421 caattttgac ctgaaaacaa cagtgggtaa ccagctgaac gggatcactg atggtttcaa
      481 aaacctgatg ggggatgtga ttcagggggc gagcggagcc gtggcgagtc tgccggcaat
      541 ggtgatacag cgggctaatc cggggctgta tgacatgctg accaacggtg tactgcaggc
      601 gaatgtggca ttcgacaaag cgcagctcaa ttgtcagaac atggcgaaac gcatgatgga
      661 gttcacggac agcaataagt ggacgcaggc ggccatgctc gaggagtaca aaaaaattgt
      721 gaatacgggg gacggtgatg cgatcaaagg ggatgaagca ggtcgtcagg ttaccgggga
      781 ggaggggcaa cgctggatcg gagggcagca gcgtggtgga gcgggacagt ctgctatccg
      841 gccgacacat gatctgacag ctgccggttt caatatgatg aacagtctgc cttcgaccag
      901 tacatccagt gtcagtcccg ggaactgtaa cgggagtgcc tgcatgaaat accgtaatgc
      961 agaggaagct gcccgggcgg tggtgaaggt tctgggggat aaatccatgc ggacctgtgg
     1021 tgatgcaaag gcatgtacca gcggggatgc tgatcagcag ccggggacca gtattgccgg
     1081 cacggggttt gcgccgatgc ttgaagaagc gacgcgggtt aacacggaac tgctggcagg
     1141 tatggtcaat ggtcgtgaca agccgacagc cgggaatctg gcaaaactga aagccgggag
     1201 tctgtctgtg acagcgggag tgatccgtgc cctgcagcgt gatccggaca atggtgccct
     1261 gacgtcgcgt ctggcgggag aactggccat ggcggatacc gttgaaacgg cgctgctgat
     1321 gcgcaggatg ctgctcacgg ggatggccga gccgtatgct gcagctcagc ctgctgcact
     1381 ggaagagggg gaacgccgta ttgaggcgct ggatcgggaa attatggcgc tgaaaaacga
     1441 gatggagctg aagcgggatc tggcgcgtaa cagtgttctg accatcattg aacgggataa
     1501 cgagcgtgtg gggaataacc cgatgatcca gcaggctgac aatgctgaca gccgtatcca
     1561 ttctctcgat gccccggata aggagtgaga ggatgagcag aatttatggc cgggtgaaaa
     1621 agaggatgaa ggtattcgcc ctgtatacgc tgttgctgac agggattacc gtgttttccc
     1681 tctggctggc cgataccggt atacgtcacg cagaaggatt tgcggccctg cgggagtgga
     1741 tgtatgagac acgttatggc tggctgggat ggcgtctgtg tctttacagt gcgatcggat
     1801 ggctggggtg gaggatctgg catgcgccgg ggttcagggc agaataccgg gcttcttttc
     1861 tgcgtatggc cacagtcagt gttctgttta tcctgctgtg tgaatacgcc atttttgctg
     1921 agccggggcg ttgagcaatc atgaccacaa ataactatct ggaatatttt ctgactctgc
     1981 tgggctgggt ggtgaataac ggtctgtgga atgtgttgat cagcacagga ctgtttgccc
     2041 tgccactggc attcaggatc atcgggatct ggctgaaagt cagggaagaa ggtgcggatg
     2101 agggaaacaa agggatgctt tccctgccgc gtattgagca tgttctctat ggtgcttttt
     2161 ttgtgatgat atcctgctgt gttcctctgg tacaggtgac gctggatacg ctgtcattcg
     2221 ataaatccag ggcacagacc tgtggggtat ggacacctga agagcccgga gagacggggt
     2281 acagtggggt gatcagcag
//
LOCUS       DAZGBC010000342         2267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGBC010000342 DAZGBC010000000
VERSION     DAZGBC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.344"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..372
                     /gene="essQ"
                     /locus_tag="ACHK1J_005145"
     CDS             157..372
                     /gene="essQ"
                     /locus_tag="ACHK1J_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7914270.1"
                     /translation="MKSMDKLTTGIAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKVARRE"
     gene            377..712
                     /locus_tag="ACHK1J_005146"
                     /pseudo
     CDS             377..712
                     /locus_tag="ACHK1J_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(876..1136)
                     /locus_tag="ACHK1J_005147"
     CDS             complement(876..1136)
                     /locus_tag="ACHK1J_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914271.1"
                     /translation="MDYTNLPKQTFADLIALRQAVVALINLLPEKEKELVKALLNRTA
                     ADFSSYPLTDDLADLPELIAASAIKLTEEIYPPQKSSQNSCE"
     gene            1250..1783
                     /locus_tag="ACHK1J_005148"
     CDS             1250..1783
                     /locus_tag="ACHK1J_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7914272.1"
                     /translation="MNVKIRYSLSAAVLALIAASAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEH"
     gene            1780..>2267
                     /gene="rzpQ"
                     /locus_tag="ACHK1J_005149"
     CDS             1780..>2267
                     /gene="rzpQ"
                     /locus_tag="ACHK1J_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO7914273.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYESLRD
                     KK"
BASE COUNT          634 a          457 c          587 g          589 t
ORIGIN      
        1 ttatttaata tatgattttc ttttaaaata attacccaca tttttaatgt gtctgttttt
       61 tagcgccgtt gagaacaacg tttgctataa aaactaaccc atagactccg atcttttcaa
      121 acatattgca ccatctgtgt acatcggggt gaggatatga aatcaatgga taagttaaca
      181 acaggtatcg cctatggcac atcggctggt aatgctggtt tctgggcatt gcagttactc
      241 gataaagtaa ctccgtcaca gtgggctgca atcggtgtgc tgggtagtct ggtttttggc
      301 ctgctgacgt atctgacaaa cctttatttc aagattaaag aagacaggcg taaggttgcg
      361 agaagagagt aatccaatga cacaagacta tgaactggtt gtgaaaggag tccgcaattt
      421 tgagaataaa gtggcggtaa ctgtagcctt acgggacaaa aaacgctttg acggtgaaat
      481 ttttgacctg gacatctcgc tggaccgtgt tgaaggtgcc gcgctggagt tttatgaggc
      541 agcagccaga atgagaatca gacaggtatt cctggatgtt gctgccgggt tatgtgtagg
      601 ggatgagcag tcgccggaaa agcgccccat aattttagag gcgcagaatg tgtggataac
      661 ctacaaagga aagctaccgg gaagaattac tggttctctg aagacgccac cgacggcatt
      721 gcggtcagaa aaagatgata ttgaatcgcc cattgaaaaa ctggagggca gcgtcgctga
      781 tctgaataaa aaattgtcgg tgctgatccc ttctgaagat gaaaaaaaac gccgcgatga
      841 gcagtttgcg gcgttttacg attattgcat tgaagttact cgcaggaatt ttgtgaagat
      901 ttttgaggag ggtaaatctc ttcagtaagc ttaatggcgg acgctgcaat taattcagga
      961 aggtccgcaa ggtcatctgt cagtggatat gatgaaaaat cggcggcagt tctgttaaga
     1021 agcgctttaa ctaattcctt ttccttctcc ggcaacaagt tgattagagc tacgactgct
     1081 tgcctgagtg cgattaaatc agcaaaagtt tgttttggta gatttgtgta atccatagtc
     1141 acctctgtgt ttatcagatt gacatcctcc tcccgccagt gcccatcact ggtgaggtaa
     1201 gatttaacat atccggggat ttgaagccga taaatcctga taaatatcca tgaacgtaaa
     1261 aatcagatac agcctgtcgg ctgctgttct ggcactgatt gccgccagtg ctcctgcccc
     1321 tgacattctc gatcagtttc tggatgaaaa agaggggaat cacaccactg cataccgcga
     1381 tggctccggc atatggacca tctgtcgggg agcaacgatg gtggacggta aacctgttat
     1441 tccgggaatg aaactgtcga aggaaaaatg cgcccaggtt aacgccattg agcgggataa
     1501 ggcgctggca tgggtggagc gcaatataaa agttccactg accgagccac agaaagccgg
     1561 tatcgcgtca ttctgtccct ataacattgg ccctggtaag tgttttccgt cgacgtttta
     1621 taagcggctt aatgccggtg atcgtaaagg cgcatgcgag gcgattcgct ggtggataaa
     1681 ggacggtgga cgtgattgcc gcattcgttc aaataattgt tacggtcagg ttattcgtcg
     1741 tgaccaggag agtgcattaa cctgctgggg gatagaacat tgaatcagat attcacggtg
     1801 attttgctcg tgttggtagg atttgtcgta ggtaatgtct ggagcgacag aggatggcaa
     1861 aaaaaatggg cggagcgtga tgctgccgaa ttatctcaag aggtaaatgt ccaatttgct
     1921 gctcgaataa ttgaacaggg gcgaactata tcccgtgatg aggctgttaa agatgcacaa
     1981 cagaaagccg ctgaaatttc tgccagggct gctgatctgt ctgatagtgt taaccagctg
     2041 cgtgccgaag caacaaaata tgccatacgc cttgacgcag cgcagcatac cgcaaatctt
     2101 gccgctgccg tcagaggcaa aacaaccaaa gccgccgaag gaatgctcac caacatgctc
     2161 ggagatattg cagcagaagc tcagctttat gctgaaattg ctgacaaacg ctacatcgca
     2221 ggagtgactt gtcaacggat ttatgaatct ttaagagata aaaaata
//
LOCUS       DAZGBC010000343         2227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGBC010000343 DAZGBC010000000
VERSION     DAZGBC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.345"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(191..409)
                     /gene="ymgF"
                     /locus_tag="ACHK1J_005150"
     CDS             complement(191..409)
                     /gene="ymgF"
                     /locus_tag="ACHK1J_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO7914274.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(541..2064)
                     /locus_tag="ACHK1J_005151"
     CDS             complement(541..2064)
                     /locus_tag="ACHK1J_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7914275.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSTTPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          673 a          492 c          468 g          594 t
ORIGIN      
        1 ttaacaaata actcttcgac atcacaatca ttgatagttg ccatagaaat tatttcaatg
       61 atatataaaa acaactgtga taacaccagg gttgacaact atttgcgcaa taattccttt
      121 ccttcaatga tccaggcgtt cactgtcagc accccattca gccgcatact gacagtgaga
      181 aactgaaaga ttattgctga tccctgtggc tgacgagtct ttcccttaat cgattaacca
      241 gattgatcgc cagccaaaca agggcattaa gcaaatttat cgtcacaagc acttctccca
      301 gcccggtact gggttttgca ataaggatga ccaggagagt gatcagcata aatgcaatgg
      361 aaaagatgat atagagagtg aaataatcca gattattact attgttcatg ctactaatgg
      421 caatcctgtc aaaactttca aacaattata gcaaacaaaa taatgaacaa tagtaacaag
      481 aggcaacgag ctaattagct gtctataatt acaccgcact atggccttac tgatcgattt
      541 tcactcaacc acaaccttca ccttcggttt agaaaggata attttgacca ggtcgatgta
      601 tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg atattatttt ggttcagata
      661 gtcaagttgt tctttcgttt cgacaccttc agcgacgata ctgatcgaaa gtttacgcgc
      721 cagatccaat acacaatcca gaattcgggt tgattctggg tctgcgttga cgcggccaac
      781 gaaactgtga tcaattttga tataatcaat atgcaggtca tgcagataag aaagccctga
      841 gtagccagta ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca gaatgttcaa
      901 ccgctgaacc agactttcat caacattcaa tggctcacgc tcggtgactt ccagaacaag
      961 gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga caaacgtcgg
     1021 cgaaataata tgcgaagcgc taaaattaat tccaatatga aaaccttccg gcagtttact
     1081 cgcgatagcg ttcatctgtc tggcaacctg attcatcagg ctttgtgtaa gcggaacgat
     1141 taatccggat ttttcagcaa gtggaataaa tgccgcgggt gaaatatatc caccgtgagg
     1201 ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac catttaccac
     1261 aggttggtaa aaagggacga tctccccatt atttattgcc cgtcgcagga tctcttcagg
     1321 cgttgtgctt ttattgaaat atctatcaag caggaaggca gcggcgcagg cgattaacag
     1381 aataaatatt aaaatcccaa agcctttatc gataagtctg tagaagctaa agagtggcgg
     1441 cgggttaaag ataatagtga aatgatattt tgtggagtga accttctcgg tatagggata
     1501 atgtccatta agcgtcatca catcacccgt tggtccaata atgtcatccg ccacgcgtaa
     1561 tacatacctt acccctttca agggtacatt aagcgccccg cgaatatgtt gatcacttat
     1621 cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag agcaggatag gtaatctatt
     1681 aacggtgtcg attgccggag ccaacagtaa attactgtcc gggacaacag gaatacgaga
     1741 cagcaggacc cgattcccag gcagggatgt acaccagact attccctgtt tgagaatgat
     1801 gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca agctgatatt gctcctcaag
     1861 gtcgcattcc ttaccggcaa tgttaaccgc tgtttgagtc gcctgtgacg cttcttcaag
     1921 tatatgattg atattgttgg cggcatatct tgctcccgcc aggtactctg cttttgcgga
     1981 ataccagagc tggatgttca aaatagcgac accggtaata aacaagcata tcgcaacgag
     2041 gatgggtatg agtgtattgc gcatttgctt gtcccggcca aagggtttaa tacatgaaaa
     2101 tgccattcat tcgctcgcgc tggcatctac agagagcatg gtagagagca taagaaatat
     2161 ccacgccgaa gataaaattt tctccatctt gatctaaccc attaattatt gagcataatg
     2221 taggcag
//
LOCUS       DAZGBC010000344         2220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGBC010000344 DAZGBC010000000
VERSION     DAZGBC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.346"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            218..1831
                     /gene="tssA"
                     /locus_tag="ACHK1J_005152"
     CDS             218..1831
                     /gene="tssA"
                     /locus_tag="ACHK1J_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7914276.1"
                     /translation="MSSPEALLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRD
                     GERGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRETGRE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDVMY
                     GDEAAIPAVVNGQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLKLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            1865..>2220
                     /locus_tag="ACHK1J_005153"
     CDS             1865..>2220
                     /locus_tag="ACHK1J_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7914277.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPV"
BASE COUNT          457 a          594 c          679 g          490 t
ORIGIN      
        1 caccctttca gagaacggct ccttcatgca tgcccgtcgg cgttttcgcc cacatgtcag
       61 aaaaaacaac cgataagctg acggaagggc aaacccactc agcaggagaa cgtatgctgc
      121 tgaaatagag gccaccacaa atcttcaaca gtaaggacga cgcttctccg gaagcggtcg
      181 tgaggtttat gaaagagact gataaacagg aaaaagaatg agttcaccgg aagccctgtt
      241 aacggcctgc gccgccagcc aggaagaaag gctgagcctc atttcccgcg caaatgaggc
      301 cctgtcgctc tgggataact ggctgcatcc atttacctcc ggatctgaaa ccggggacga
      361 tcctgcatac gatgataatt ttcagctgat gcgtgaagag atcaataaga tttcaggtac
      421 tgattcagcg ctgttgtgcg aactggctca gaagtgcctt tgcgaatgcg ccagagatat
      481 tcgtgtcgtg acctggtacg tgctggcccg tctcacccgg gatggtgaaa gagggttgtc
      541 cgaaggctta ttacttcttg ttgcgatgct gacccgctac ggacaggcat gtcatccccg
      601 gcgtcctgcc gcccggaaag ctgcgctgga gtggctgaac agtgcaaaag tgactgatgc
      661 actgctgtta tggcccgggg tggacagcaa tgaggccggt ctgacagcgg gtgccataag
      721 cctgctggga tccgccgtgg cagactggcc ggaagcggag aagccgtctt ttgcggggct
      781 ttgttccgcg cttgagaacc gcctggcacg ttcagggggc atggaggcac tggtccccca
      841 gaacagcagt ccccgggaga ccgggcgtga atcagctccc ccggactcac cacaactggc
      901 agcggtgacc tcggggcgcg acttactcga ccaggccagg ttactttccc gctggctgag
      961 cgggcagccg cagggctggc tggcttctca ccggctgatg aagacggtgc ggtgggatac
     1021 ggtcgatcag ctcccttcgc tggacagtag cggacgcacc cgcctggtcc ccccgaaaac
     1081 cgggtatcgc gctcagctta agcgactctg gcttcagaaa aactgggttg agctgattga
     1141 actggccagt cagatgtatt gcgaaggtgt gaatcatttc tggctcgatc ttcagtggta
     1201 tctctggcag ggactcagtc atgctggtca gccctgggat tcctggtctg acgctgtcct
     1261 gtcagatctg cggctgttgc ttaaacgtct cccgggcctt gaaggactgg catggaatga
     1321 cggtactccc tttgctgacg aggtcaccct cgcctggatc gctgaaaaag tgaatgaaga
     1381 agatgtgatg tacggcgatg aagctgccat tcctgccgtt gttaatggcc agactgacga
     1441 tgtgctgatc cttgagtctg aagccatgga aaagggagac acggaagggc ctgaagcggc
     1501 gcttgcctgg ctccagtccc ggccggggat ggatacacca cgtcatcgct ggctgatacg
     1561 cctgctcatg gcccgggtgg cagagcagta tggccggaac gatatggcgc ttcatctgct
     1621 tggtgaactt attacccgtg cgccacagct gacgctggaa gactgggagc ccgctttgct
     1681 gtttgaagtc cgggcccgac gcctgaagct gctacggatg aaagccggtc gcagcgaatc
     1741 tgataaagcc cgtctgatgc ctgagatgga tacgctgctg gcggggctga tcgccattga
     1801 cccggcccgt gccatggtgc tctgtggctg atgttctctt taatcatttt caggaatgcc
     1861 gtgcatggat gatttaaccc tgcgctatta cgaagcagag atgcgctatc tgcgggaagc
     1921 cggtaaagag tttgcccgtg ctcatccgga tcgggcagca atgctgaatc tggataagcc
     1981 tggcgcctgc gatcccggtg tggaacgtct gtttgaaggc tttgccttcc tgatgggacg
     2041 tttgcgtgaa aagctggatg atgacctgcc ggagctcacc gaagggctgg tgagtctgct
     2101 gtggccgcac tatatgcgga ctatcccgtc gctggccatc gttgagttct cgcctgactg
     2161 gcgtagtctg cgtcaggccg aaatactggc tgaaggcttc tccgttttgt cccgtcctgt
//
LOCUS       DAZGBC010000345         2215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGBC010000345 DAZGBC010000000
VERSION     DAZGBC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.347"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /locus_tag="ACHK1J_005154"
     CDS             <1..216
                     /locus_tag="ACHK1J_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914278.1"
                     /translation="PLSENGSFTPVGVFAPMSEKTTGKRPEGQPHSAGESMLLKWRPP
                     QIVNSKDDASPEAVVRFMKETDKQEKE"
     gene            213..1826
                     /gene="tssA"
                     /locus_tag="ACHK1J_005155"
     CDS             213..1826
                     /gene="tssA"
                     /locus_tag="ACHK1J_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7914279.1"
                     /translation="MSSPESLLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLIRD
                     GEKGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAERPSFAGLCSALENRLARSGGMEALVPQNSSTRESGGE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLNGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDMMY
                     GDEAAIPAVVNDQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLKLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            1860..>2215
                     /locus_tag="ACHK1J_005156"
     CDS             1860..>2215
                     /locus_tag="ACHK1J_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7914280.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     SVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPV"
BASE COUNT          458 a          587 c          673 g          497 t
ORIGIN      
        1 cccctttcag agaacggttc cttcacgccc gtcggcgttt tcgcccccat gtcagaaaaa
       61 acaaccggta agcgaccgga agggcaaccc cactcagcag gagaaagtat gttgctgaaa
      121 tggaggccac cacaaatcgt caacagtaag gacgacgctt ctccggaagc ggtcgtgagg
      181 tttatgaaag agactgacaa acaggaaaaa gaatgagttc acctgaatcc ctgttaacgg
      241 cctgcgccgc cagccaggaa gaaaggctga gcctcatttc ccgcgcaaat gaggccctgt
      301 cgctctggga taactggctg catccgttca cctccggatc tgaaaccggg gacgatcctg
      361 catacgatga taattttcag ctgatgcgtg aagagatcaa taagatttca ggtaccgatt
      421 cagcgctgtt gtgcgaactg gctcagaagt gcctttgcga atgcgccaga gatattcgag
      481 tcgtgacctg gtacgtgctg gcccgtctta tccgcgacgg tgaaaaaggg ttgtccgaag
      541 gcttattact tcttgttgcg atgctgaccc gttacggaca ggcatgtcat ccccggcgtc
      601 ctgccgcccg gaaagctgcg ctggagtggc tgaacagtgc aaaagtgact gatgcactgc
      661 tgttatggcc cggggtggac agcaatgagg ccggtctgac ggcaggtgcc ataagcctgc
      721 tgggatccgc cgtggcagac tggccggaag ctgagaggcc gtcttttgcg gggctttgtt
      781 ctgcgcttga aaatcgtctg gcacgttcag gaggcatgga ggctctggtc ccccagaaca
      841 gcagcacccg ggagtccggg ggggaatcag ctcccccgga ctcaccacaa ctggcagcag
      901 tgacatcggg ccgcgactta ctcgaccagg ccaggttact ttcccgctgg ctgaacggac
      961 agccgcaggg ctggctggct tctcaccggc tgatgaagac ggtgcggtgg gatacggtcg
     1021 atcagctccc ttcgctggac agtagtgggc gtacccgcct ggtacccccg aaaaccggat
     1081 atcgcgctca gcttaagcga ctctggcttc agaaaaactg ggttgagctg attgaactgg
     1141 ccagtcagat gtattgcgaa ggggtgaatc atttctggct tgatcttcag tggtatctct
     1201 ggcagggact cagtcatgct ggtcagccct gggattcctg gtctgacgct gtcctgtcag
     1261 atctgcggct gttgcttaaa cgtctcccgg gccttgaagg gctggcatgg aatgacggta
     1321 ctccctttgc tgacgaggtc accctcgcct ggatcgctga aaaagtgaat gaagaagaca
     1381 tgatgtacgg cgatgaagct gccattcctg ccgttgttaa tgaccagact gacgatgtgc
     1441 tgatccttga gtctgaagcc atggaaaagg gagacacgga agggcctgaa gcggcgcttg
     1501 cctggctcca gtcccggccg gggatggata caccacgtca tcgctggctg atacgtctgc
     1561 tcatggcccg ggtggcagag cagtatggcc ggaacgatat ggcgcttcat ctgcttggtg
     1621 aactcattac ccgtgcgcca cagctgacgc tggaagactg ggagcctgct ttgttgtttg
     1681 aagtccgggc ccggcgtctg aagctgctac ggatgaaagc cggtcgcagc gaatctgata
     1741 aagcccgtct gatgcctgag atggatacgt tgctggcggg gctgatcgcc attgacccgg
     1801 cccgtgccat ggtgctctgt ggctgatgtt ctctttaatc attttcggga atgccgtgca
     1861 tggatgattt aaccctgcgc tattacgaag cagagatgcg ctatctgcgg gaagccggta
     1921 aagagtttgc ccgtgcccat cctgatcggg cagcaatgct gaatctggat aagcctggcg
     1981 cctgcgatcc cagtgtggaa cgtctgtttg aaggctttgc cttcctgatg ggacgtttgc
     2041 gtgaaaagct ggatgatgac ctgccggagc tcaccgaagg tctggtgagt ctgctgtggc
     2101 cgcactatat gcggactatc ccgtcgctgg ccatcgttga gttctcgcct gactggcgta
     2161 gtctgcgtca ggccgaaata ctggctgaag gcttctccgt tctgtcccgt cctgt
//
LOCUS       DAZGBC010000346         2192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGBC010000346 DAZGBC010000000
VERSION     DAZGBC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.348"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..187
                     /locus_tag="ACHK1J_005157"
     CDS             <1..187
                     /locus_tag="ACHK1J_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914281.1"
                     /translation="RWRQEGGLLRHQAQFPHTPSGVQGAQVTARLKKNDGTTMACRGH
                     WRTTPAQVKTEKTARHL"
     gene            295..546
                     /locus_tag="ACHK1J_005158"
     CDS             295..546
                     /locus_tag="ACHK1J_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7914282.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(577..822)
                     /locus_tag="ACHK1J_005159"
     CDS             complement(577..822)
                     /locus_tag="ACHK1J_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914283.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFEEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(822..1760)
                     /locus_tag="ACHK1J_005160"
     CDS             complement(822..1760)
                     /locus_tag="ACHK1J_005160"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7914284.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQKGRM
                     EEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            complement(1825..2091)
                     /locus_tag="ACHK1J_005161"
     CDS             complement(1825..2091)
                     /locus_tag="ACHK1J_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914285.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
BASE COUNT          487 a          585 c          586 g          534 t
ORIGIN      
        1 gaggtggcgg caggagggag gactgctgcg ccatcaggcg cagtttcctc acacaccgtc
       61 aggtgtgcag ggagcgcagg tcacggcgcg actgaagaaa aacgacggca caacaatggc
      121 atgtcgtggt cactggcgca ccactcccgc acaggtgaaa acagagaaaa ccgcacgaca
      181 tctataacta aaaaaagtga aatttcttca ctttttacac ttatttatcg cgctgatttg
      241 gcaattgtga tgataaaata aagtgaagtt ttttcacttt ttcaaaggtg ttttatgacc
      301 ggatgggaac tgcgaatctg gcgaaaaagc atgctctggt cacgcgaaaa agcggccaga
      361 gaatttggcg tgacgcagag aacatggcac gcctgggaaa atgcggaaca ggtggacgtc
      421 accgtctgga ggaccaccca ggcactttca gtccgtgacc tgctgcctca catgcagggg
      481 atgcgcaagg cagatatcat caggcgtctg gaaaatgaac tgggggaaac ggcagggaat
      541 gtgtgagcgt gattcacagc ctggcagaat gccaggctac tgctcatcag gaaacagctt
      601 tttcagttct tcaccggaaa gtccggttgc ctcctgaatc cgttccggcg tccagcccat
      661 atcccgaagc cggcaggctg cttcccgcgc cacttcaagc gcacctttct taaaaccttc
      721 ttcgaaacct ttctgtataa gacgttctgc aatcgtcatg ctgtttgctc tgctcctgct
      781 gacaaaaaaa cccggcattt tgccgggctt tgtggttctc atcagtgagt gatgttcttc
      841 agttcagctt cggaaagtcc ggtgatctga cagatttgtg cagatggcat tcccgttttc
      901 tgaagctgac gcgcaatatc ctgcttctcc tccatgcggc ctttctgtat tccctgctgt
      961 attccctgct gtattccctg ctgtattcct ttctgtattc cttgctgctc aagacgttcg
     1021 gcaatagtca tcagtttatc ctcatgctgc ggcagacgct cagccagcgc ccgcataaat
     1081 tccggtgata cagagtcccc tgcgttaacg atgtaatgaa tcagaacacg cacctgctcg
     1141 tctgaaattt ccaccatgac ctgcgggagt ttgtccaaca gctccatcat gtcacgatgg
     1201 cggatatgct tcatcagcag cgtcagtgcg gccatccggc gatggttcat gatttcattg
     1261 tcatcaacga cagtgatatc aaccagcgga aacggctttg tgtatatttt agccgcaagt
     1321 gccggatcct caaaacagtc cagccagttc atgctgtacg ggtacgggct gcgctcaccg
     1381 tgatagaaca gcaccggaat gaccagtggc aacgttttgt gctgctccag atgtctctgc
     1441 atcgccgcca cactgtaacg taacatgcgg aaaggcatgt acagattttc ggtgctctgg
     1501 tgctcgatga gcgtataaac atagccgtct tcccccccgg tggttttcac actccagagg
     1561 atatcgctgg cgtactgatg caggtccggc tcaacaaagg tggcgggttc cagcttcagc
     1621 gtggacaggt cgcacaactg ccggtactcc gccggaagat gcagttccag aaaatctctg
     1681 gcgacgtcgg gattcgccag gaacgaccgg aacgcggcat catgcggcgt gggcgttgtg
     1741 gtgtttttct tcttactcac ggttcctcct ggtgagatgt ctgaacgtat cctgccgcct
     1801 gcacaggcag acggcaaggg ttaatcagtg acagacccgc tcaatttcaa gcgcgcggag
     1861 caggtggccc cattcgctga ctttcatggc ggggatacgt acccgcttct gctcactgca
     1921 gcgtctggcg atatgaccgc ccatcgccag gacgtccggg ttaatctcat ccggtgccag
     1981 ttcctggcgt ctgaagcggg tatggcggtg caggggtttg ttctcccgca ggcactcttc
     2041 acgcagacgc tttacatact cggcggttgc gaagataatt gatggtctca tggcataatc
     2101 tccatagtta gtgaatgaga tgccggactt ccgtacgggg aagcgccggc ggagaaaggc
     2161 agggtttatc gccctgcttt ttttcattcc gg
//
LOCUS       DAZGBC010000347         2180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGBC010000347 DAZGBC010000000
VERSION     DAZGBC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.349"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..715
                     /locus_tag="ACHK1J_005162"
     CDS             <1..715
                     /locus_tag="ACHK1J_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn7 transposase TnsA N-terminal
                     domain-containing protein"
                     /protein_id="HFO7914286.1"
                     /translation="RIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTDI
                     SGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPDL
                     LIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEEH
                     AISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIIP
                     KISNNTSNQRQPNESDCHN"
     gene            complement(854..1006)
                     /locus_tag="ACHK1J_005163"
     CDS             complement(854..1006)
                     /locus_tag="ACHK1J_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7914287.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            1439..1642
                     /locus_tag="ACHK1J_005164"
     CDS             1439..1642
                     /locus_tag="ACHK1J_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO7914288.1"
                     /translation="MRTTVTIDDALYAQALDMAEPGMDKSDIFREAVKTFIRVQAAKR
                     LASLGGTAPDMEITSRRREDAVE"
     gene            1639..2013
                     /locus_tag="ACHK1J_005165"
     CDS             1639..2013
                     /locus_tag="ACHK1J_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO7914289.1"
                     /translation="MILVDTSVWVDHFKNKNDTLIQLLLSDCVLMHPMILAEIACGTP
                     PAPRQQTLSNLDLLPKSHQATMTEVLAFIENEKLFGSGCGLVDITLLASTRITPGARL
                     WTLDKRLSRLADILNIGFQPVH"
     gene            complement(2102..>2180)
                     /locus_tag="ACHK1J_005166"
     CDS             complement(2102..>2180)
                     /locus_tag="ACHK1J_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7914290.1"
                     /translation="DSLCGFRVEQGNTVLVATLAYEVKR"
BASE COUNT          652 a          492 c          471 g          565 t
ORIGIN      
        1 ccgaatcaag aaagaaagag ttgagttcat attcagtaaa tggctagaca atacacattg
       61 gccaaaatat atcgcacctc ctccccccgt agaacagcta tgttcacaga gagcggttaa
      121 tgatactgac atatctggat tcttttactc tcaaaaatta ggtcgtacag tccagtacga
      181 atcagggctt gagtttaaaa taatgaatct attagaatgt tgcgatcagg ttcttttcta
      241 tcaagaacaa ccattttcta ttccatattg ttttaaaaac aaatcaaaaa aatactatcc
      301 agatttactc attgcgacag ttgatggtcg ctgcctactt atggaagtga agccaacaga
      361 ccgaatgacc ttgagcctca cgagaataaa aagtaatgca ggccgcaaat gggcacatac
      421 tcgaggatgg ggatggttgg tcattagtga taggttctcg ttacgtcagt tggaagaaca
      481 tgccatctca acaaacacat ggaaacttat agaggctgaa ctgaaaacat caaggatttt
      541 agcatggcgt gaaatgatgg agttgcgtac acgatacgag attcatcgtt tagatcttat
      601 tgcatatatc attcagtctg gtgctgaagt ggataacttt tatcgaatta tcccgaaaat
      661 atccaataat acatctaacc agagacaacc aaatgaaagc gactgtcaca attgatgacg
      721 tactggacgg aaacacatta tagctgccgg acagcagcaa ccagcaatca tccggtaaaa
      781 aagacaaaca gggcgcagtg aggtctgcct tcgcaacaat ccggcgccag atttcctgcc
      841 gccgtgttgc ccgttaacgt ttaacttcgt aggctaacgt tgccacaagc actgtgtttc
      901 cctgctcgac acggaatccg caaagcgaat ccctcaccat ccagacaaaa cacagtatcg
      961 tcacacagac gacaattaac attgttaaaa acgttcgttg tggcatcgca gcccctccat
     1021 tgcctttcgg ccgtcatcgg gctactataa tgttgtcgat gcattacagt agagccctgg
     1081 gttgattgat aaaaaaatcg cctggggctt tctgctgcat gccgtcccca aaacgctttc
     1141 ggcatacagc ctcaagcgcc cgccgctatt ctaatcgttt tcctgtccgc cacaatcctt
     1201 ttcatgccgt gatgtttttg gtatccggga caggaaaaat gtcgtgaatt atccaggagt
     1261 actggcgatg aacatgttca gttgcaggct ggttttggtc attctctata ccatcctgtt
     1321 gttcagggca tcaaaaagtt tcttttccat aaatcagact ccaacaacat ttgacacgat
     1381 gcattatgtg atgcacattt gaatcatata atgcatcaaa tgagaagaga gaaacaccat
     1441 gagaacaact gtaacaatcg acgatgctct gtacgcacag gctctggaca tggctgaacc
     1501 tggtatggat aaatcagata ttttccgtga agcagtgaaa acatttatcc gtgtacaggc
     1561 agcaaaacgt ctggcatcac tcggcggaac tgcgccagat atggaaataa catctcgcag
     1621 acgtgaggac gcagtggaat gatattggtt gatacgtctg tatgggttga tcattttaaa
     1681 aataaaaatg atacattgat tcaactattg ctgtcggatt gtgttcttat gcatccaatg
     1741 attctggctg aaatcgcatg tggcacaccg ccagcaccca gacaacagac attaagtaat
     1801 ctggacttgt tgccgaaaag ccatcaggca acaatgaccg aagttttggc atttatcgaa
     1861 aatgaaaaat tatttggttc aggttgtggg ctggtcgata taacactact ggcgtcaacg
     1921 cgaattactc ccggagccag actatggaca ttagataaac gcctgtcacg gctggcagat
     1981 attcttaata ttggatttca gccagtacat taaccccgca gggataataa aggaaaaagg
     2041 gcgcagtgag gtctgccttc gcaacaatcc ggcggcagat ttcctgccgc cgtgttgccc
     2101 gttaacgttt aacttcgtag gctaacgttg ccacaagcac tgtgtttccc tgctcgacac
     2161 ggaatccgca aagcgaatcc
//
LOCUS       DAZGBC010000348         2169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGBC010000348 DAZGBC010000000
VERSION     DAZGBC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.350"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(76..873)
                     /gene="mtfA"
                     /locus_tag="ACHK1J_005167"
     CDS             complement(76..873)
                     /gene="mtfA"
                     /locus_tag="ACHK1J_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO7914291.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            967..1056
                     /locus_tag="ACHK1J_005168"
     tRNA            967..1056
                     /locus_tag="ACHK1J_005168"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1001..1003,aa:Ser,seq:cga)
     gene            complement(1626..2156)
                     /gene="yodB"
                     /locus_tag="ACHK1J_005169"
     CDS             complement(1626..2156)
                     /gene="yodB"
                     /locus_tag="ACHK1J_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO7914292.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          630 a          470 c          474 g          595 t
ORIGIN      
        1 ttagcggtac cttgtctgtc aacactaaat ttcataagtc atttcaattg gttaattaat
       61 ctgcaaagtt gttaattaat gaacattcgt cgccgaaaac gagtctgtat cattagcgtg
      121 atgcagtctc tgcaaaggat cttgttgata aaattggcag aaacgttgcc acaatgaagg
      181 aaaacgagga gcaaaaagtt ctggggcgct aaagaaatat tcagaaagta cggcaaaaca
      241 ttcagcagga tcactggcag cataagcatc aatgctcgcc gcattctcgc caaccaattc
      301 gatttcttcc tgaatgttgt tcattgcagc atgaagatcg tgttcccagc cagcaacctc
      361 acgcaacgga ataaagggaa ctccgctggc gcgatcgccg ttacgggtgt ccagcttatg
      421 agcgacttca tgaataatca ggttaaaacc agaagcatca aaagaatctt gtatatccaa
      481 ccagttcaaa acgataggcc cttgctgcca gctctgacct gactgaacaa tacgttggtt
      541 atgcaccaga ccgatatcgt cttcccattc atcatcgacc acaaatggcg caggataaat
      601 taagacttca tgaaaaccat ccagccattc cagtcctaac tccagaacgg gtaggcaaaa
      661 tagaagtgct atccggcagc ttcttaatga atccagttca aagccctgta aaggaacaag
      721 ccgcttttgc tgtaaaaaac gttcggcaag agtgactaat ttgctttgtt cctgttccgt
      781 cagacacgtt aaaaggggga tcgatagtgc ttcctgccag ggaagggcag tttgatgtgc
      841 tgattcttgt actttccagg gccacttaat catcgttttg ctcgcaaact cgtcacttga
      901 acaaaattgc acggacaggg actgttaaaa tgccaaattt cctggcatca tggcaaccat
      961 ctgaacggag agatgccgga gcggctgaac ggaccggtct cgaaaaccgg agtaggggca
     1021 actctaccgg gggttcaaat ccccctctct ccgccacttt atcaatgact tatctcccga
     1081 cttcccgcct tgcttttcct aaacagaaca atcgtagaat attcttgaag ggttagatcg
     1141 tcactgtttt ctgttcgata ctgtgacatt cagcacttga ttcgctatgg atctgacagg
     1201 aagctttcga gcgaaaatgt gcagttattc agtcgttttc ttatcgctca ccattattct
     1261 tttagacatt catcctatga aactgccgca aagttggtgg tgggaactga agttgcgtag
     1321 agaaggggtc aataattgag tcaaaaaagg aacgcggccg tcagattaaa gccgcgggac
     1381 aaagtccata atttccaaga gttgttgaat ttcccagctt ttttgaaagc ctttgtatca
     1441 tactaacaag taaaaaaggt ttaaatacca aaattaacct gtatttttga taagtcttac
     1501 cattgtacga tttacacagt ttctgctcct ccttgttgat agtggaaagg gtgcattgtg
     1561 cacttgcgta aacatttatg acgcgttgat gtccatggtt cacatttcct tgtcagggga
     1621 tcgacttatt tgcggcgagg catcattcgt aacagagtat tatctttctg aatgtagtga
     1681 tgaaagagtg ctgcgccagc gtgagctgcg attaaaaagt aacctatatt tgcccaggtt
     1741 tcgtgaatat tttttatcag tgctttaatt tcgctgtttg gggtaacaaa gggagacaca
     1801 ttgaaaccaa ggaaactcca cgattttcca ctgtaagcca ttaaagcaat ccccagcaga
     1861 ggaagcgcaa ggaaggttat ataaagcatg atgtgcatta gcgaagccgc tttcatttgc
     1921 caggcaggtg gcggtggcac aatagaagga tcactataac ggtgttttat aatcaggcgt
     1981 gaaaacatta acacccaaac gaatataccc gcattgtaat gtgtttctcg catcagcaga
     2041 taagtactac tacctttagg aaaccagcca cggagttcca tcgcggcata ggttattgca
     2101 acgaaaagca gcgttatcca atgtaaataa atttgagttt ttgagaatcg gttcattttt
     2161 gtagctcct
//
LOCUS       DAZGBC010000349         2163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGBC010000349 DAZGBC010000000
VERSION     DAZGBC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.351"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            152..661
                     /locus_tag="ACHK1J_005170"
     CDS             152..661
                     /locus_tag="ACHK1J_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914293.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
     gene            750..1373
                     /locus_tag="ACHK1J_005171"
     CDS             750..1373
                     /locus_tag="ACHK1J_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7914294.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYYL"
     gene            1462..1695
                     /locus_tag="ACHK1J_005172"
     CDS             1462..1695
                     /locus_tag="ACHK1J_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914295.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1715..1924)
                     /locus_tag="ACHK1J_005173"
     CDS             complement(1715..1924)
                     /locus_tag="ACHK1J_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7914296.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
BASE COUNT          510 a          486 c          571 g          596 t
ORIGIN      
        1 aatttagtta ttattcctca ctgattaatt gtccttccgt tcttattgtt tgtaacttgt
       61 gtttgtatcg tacctttcag gtgttgatgc gcgctttact gtgtctgcac cggcttgaca
      121 aaattcaaca ggggttgaaa aggaacattt cgtgcaaata accgaagcct taatttcaga
      181 gccgggggaa atccggcgtt ttgttcagca ggcggtggac cactggccaa atctgttggc
      241 atttcacttc acgctctatt cggcagaagg aatctatggt caacagattc agacattttg
      301 tagttccttt caccgacggg tacacgaacg tattacggag cataatcaca cggtcagccc
      361 atcagcgccg gtggtattac gctggttgcg ggaacaacat gaaggagcac agatacgatg
      421 tctgttgctg ctcagccaga cgagtatttg ccatccgcga gtcggcgtca tggctgatga
      481 agaatgtgcg caactggtgg atttactgca acagacctgg tcggtgataa gtgctggcgg
      541 acaatgccgg gtggaaagat gttttcgggt tgcccggcct ggttcatcag ggcaatacgt
      601 ggcgttaaaa acggctgttc agtctctgat gccacggggt attgcaacaa tcatccggtg
      661 atggacggga gaaatatcga ctcttccgtt agtttttaca gactgataaa gttttgccag
      721 ctgaatcttt atacggaatt cttttcagta tgcgtacacg aattgactat cttgcggata
      781 aatacagttt tactgaactg aatgaacttc cacgccttca ccagcagtgg caggatgttc
      841 tggaggagtg ccggcagaca gaggccgggc cggaagaacg gctgcgtatt gcactgctga
      901 atgtggatta cgtcaccagt tttgaactgc cttttcgctt gttgcttacc cggacaccac
      961 aactgattgc cgcgctgcgt gaagaatggg atatcagcca gaaaaatgtg gtgttcaacg
     1021 ataaacgctt tggctgcgtg tacagcctga aggccagcct ttccggtgtg ccggatacat
     1081 accggtatca tctgtctcat cgtattcgcc gggtgattgg gaatgaaaac acatcatcgc
     1141 cataccagca gattgcccgg gaagtgaaag cgccccgtga acggctgaag catgcgctgg
     1201 aggcggggtt gctggtgact gcactggacg gcctgttctg gttcggcagt cagcgtattg
     1261 cggctgatgt cctgaggttg agaaagagcg gaatgccggt ggtgacaacg accgcggagg
     1321 tgcatgataa tctgacggga acaacccgca aagtaccggc ctactatctc tgattcgacc
     1381 atcaggggga atattgctct ggcagcagca gtacgacatt ctttatcccc gagcctgaaa
     1441 acatgtgcta aggaaacggg aatggcaagg tccgattacg atattatcaa tctgtctccg
     1501 gaacatgaac tgaatgagtg gctggcagag agaggttatg ccggacaggc ggataaccgt
     1561 aaccgggtgg cagaaattgt tacccgcaaa ttgcaggaca gcttttatat caacgtttcc
     1621 tgggatgcgc tgaacacggc atacagtgaa cacccggagt ggttttctgg gcttgtctcc
     1681 ggggatgaga attaataagt ggattatgct gccatcaggc agtgtgattg ccggcagggg
     1741 gttctttccg gggttcgggt ttgaattttc ccaggaagtt gtacatcggc cgcatgcagg
     1801 gatgcggata gccgtctcgc ataaacacca cccgggtttc ctcaacactg gtcacggtga
     1861 caagcgcacc atgatcatct ctgtatcgct gattgatttt cggtaatgga atatcttctg
     1921 acatagattt cagaacggta accgttcact aaattttcat gtggtatcac tgaatcagga
     1981 gacgacgttt cctgatgtgc gttgttaatt ttctgttttt aaaaaatatt ttctctctct
     2041 tctttatttt ccgtgtcttt tattgctctc ctctttcatg tccttctctt tttctgatat
     2101 gcccttaaat gctgccgaat catcactcct cgtgaatgtc attgctggta atcattcggt
     2161 ata
//
LOCUS       DAZGBC010000350         2161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGBC010000350 DAZGBC010000000
VERSION     DAZGBC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.352"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..>2161
                     /locus_tag="ACHK1J_005174"
     CDS             11..>2161
                     /locus_tag="ACHK1J_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7914297.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSSASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAADSKTAAASSASAASTSAGQASASATAAGKSSESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSETLAATPKAVKTANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGA"
BASE COUNT          605 a          590 c          621 g          345 t
ORIGIN      
        1 tggggtgaat atggcagtaa agatttcagg tgtactgaaa gacggcacag gaaaaccggt
       61 acagaactgc acaatccagc tgaaagcaaa acgtaacagc accacggtgg tggtgaacac
      121 gctggcctca gaaaatccgg atgaagccgg gcgttacagc atggacgttg agtacggtca
      181 gtacagcgtt attctgttgg tggaagggtt cccgccgtca catgccggga ccatcaccgt
      241 gtatgaagat tctcaacccg gtacgctgaa tgattttctt ggtgccatga ctgaggatga
      301 tgtccgtccg gaggcactgc gccgttttga gctgatggtg gaagaggtgg cgcgtaacgc
      361 gtccgcggtg gcacagaaca cggcagccgc gaagaagtca gccagcgatg ccagcacatc
      421 agcccgtgag gcggcaaccc atgcgactga tgctgcggac tcagcacgcg cagccagcac
      481 gtcagccgga caggccgcgt cgtcggctca gtcagcgtct tccagcgcag gaacggcatc
      541 aacaaaggct actgaagcat caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc
      601 ggctaccagt gccggtgcgg cgaaaacgtc agaaacgaat gcggcagcgt cacaacaatc
      661 agccgccact tctgcatcca ccgcgaccac gaaagcgtca gaagctgcct cctcagccag
      721 ggatgcgtcg gcttcaaaag aggcggcaaa atcatcagaa acgagtgcag cctcgagcgc
      781 cagtagttcc gcttcctcgg caacggcggc aggcaattcc gcgaaagcgg caaaaacgtc
      841 cgagacgaac gctaagtctt ctgaaacggc agcggaacag agtgcctccg cagcagcaga
      901 ctcaaaaaca gcggctgcat catctgccag cgccgcgtca acaagtgccg ggcaggcctc
      961 agccagtgcc accgctgccg gaaagtcgtc agaaagtgcc gcatcgtctg cttcaacagc
     1021 cacaacgaag gctggcgaag ccactgaaca ggccagcgca gcagcgagtt ctgcttccgc
     1081 agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc agcgcagaat cctcaaaaac
     1141 ggctgccgca tcgtcagcca gttcggcggc gtcatcggca tcatctgcgt ctgcttcaaa
     1201 agatgaggcg accagacaag cgtcagcagc gaagagcagc gccacgacgg catccacgaa
     1261 ggcgacagag gcagctggta gtgcgacggc agcagctcag agcaaaagta cggcggaatc
     1321 tgcagcaacg cgcgctgaga cagcggcaaa acgggcagag gatattgcat ccgccgtggc
     1381 gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagcagtg cgaccaacag
     1441 cacttccgag tcactggcgg caacgccaaa agcagttaag gccgcgtatg acctggctaa
     1501 cgggaaatac acagcacagg atgcaacgac agcacagaaa gggatagtcc aactaagcag
     1561 cgcgaccaac agcacgtctg aaacgctggc ggcaacgcca aaggcagtaa aaacagccaa
     1621 tgacaatgct gagaaacgtc tgcagaaaga tcagaacggt gcggatatcc ctggcaaaga
     1681 cacctttacg aaaaatattg gtgcctgccg tgccttcggt gggtcagtaa gcacaacaac
     1741 aggaaattgg acgactgcac agtttatcga gtggctggat tctcagggag catttaacca
     1801 tccatactgg atgtgcaagg gttcctggtc ttatggcaat aataaaatca ttactgatac
     1861 tggctgcggt aatattcatc tcgccggagc tgtcattgaa gtaatgggga taaagtcagc
     1921 gatgacgatc cgcattacca ctccgaccac atccagcggt ggcggaacaa ccaacgccca
     1981 gtttacctat attaatcacg gaacagatta ttcacctggc tggcgaaggg actataactc
     2041 cagaaataag ccaacggcat cagagatcgg ggcgctaccg tcaggtggaa cagcagtatc
     2101 atcagttaat ctggcttcaa aaggtcgggt agccgcgctg acagacaata cgcagggggc
     2161 a
//
LOCUS       DAZGBC010000351         2151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGBC010000351 DAZGBC010000000
VERSION     DAZGBC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.353"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..792)
                     /locus_tag="ACHK1J_005175"
     CDS             complement(31..792)
                     /locus_tag="ACHK1J_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO7914298.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1582..>2151
                     /gene="subA"
                     /locus_tag="ACHK1J_005176"
     CDS             1582..>2151
                     /gene="subA"
                     /locus_tag="ACHK1J_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO7914299.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRK"
BASE COUNT          599 a          499 c          468 g          585 t
ORIGIN      
        1 ggtaattatt atctttcagg agtagtttcg tcagaagtta taagtcacac caagcatgat
       61 gtcatgactt ttaacatcaa cttctgattt atatttatcg ccccactcat ccttgtaact
      121 cacactggca tcacctgcat caagatagcg atagctgagg tccagagcga tatccggggt
      181 tacgtcatag cggacacctg ccccaaaact ccatgcaaaa ttgttatcat atcccgggta
      241 atgcaacgca gaaaaattgc tcacctcacc agatttattc caggaggtat cagtatatgt
      301 tgccttatga tgtatccgtg cataacccag tccaacagac acccatggag tgaatgcact
      361 gctggtgaat gcactgctgt ttctgaagtc ataatacgta ttcaccatca gagtattcac
      421 actgagcctg ttctgtgtgt cttcccgacc accaccgacc atcggagagt accatgtatc
      481 cagtgtataa cgggagtccg cagctccgcg gccatagaat tccacttccg tgcgcacagg
      541 aacattgtaa tgctgataaa aatcatagcc caccgcaagg ccggcaccga ataccgtatc
      601 gttcttatcg ccgcccttat acttataaac atcgtcccca aagtcctgac gaaagcgctg
      661 gtcagtctga gtcacaaccg aagcaccagc tttaccggtc atataaaagc ctgttttgcc
      721 tctatcagcc agggccaggc ctgaaaacat gcccaccaca gcaagcgctg aaaccgcaat
      781 aatctttttc atttcagtta tctccgctat cactttcccc gaacgataaa cgtggttgct
      841 tattctaaag ctatgcaaaa gacagggtaa aaagagaaaa atcgatcgaa tcgatcgaca
      901 aaatcgatca tgcatgatga agatttctta tcgaatacat aaaaatagtg atggcaatcg
      961 tggcaggagc ttacgtcagg ccctgaagta ctgacacagg cattgatggc aggtgatata
     1021 aggagtgctc cagggaatga ctcccgactg acccaggcac tgtcccgttg tcaacccgta
     1081 tggttactgc tctgactcat ttcctgtcgt cagacgttca actctggacc actggttatg
     1141 acgacaggtg tcgctcattt gtcagcacta taacggtcaa agatcgtggc attgacacat
     1201 tctccagagg taaatttctc aagaggtaac aactctgcca ggaggatatc tgcagagagt
     1261 tacattcgtt tgggttcata tctgtgccgc gcgttttcct tccatcagtg aacaaacatt
     1321 cagtcccatg acgaccgggc aaatagcgtg ttcacagata ttgctgtcca ggtacgaagt
     1381 accataatgg atattggtaa gttattgggt gaaccccctc catgaaaaaa ccaaatataa
     1441 aaatgaatgt ttcgggagag acttcactcg ctttattgat agtgattgtt tatcttttac
     1501 tgaagacagg atatttcaga tgtgatatcc ctgtaacata ttgaccagca atacttttta
     1561 tttgtaccac tggaggatat tgtgctgaaa aatttacggc tgcatattct gttcctgctt
     1621 tttatctcag tgtcagtcag ggcggaaaaa ccatggtatt ttgatgcaat tggtctgaca
     1681 gaaacaacca tgtcgctcac agacaaaaat accccggttg tcgtatctgt tgttgattcc
     1741 ggagtggcat ttgtaggagg cctcagcgac agcgaatttg ccaaattcag ttttactcag
     1801 gatggttcgc cattcccggt aaaagagcct gaagctttat atattcatgg tactgccatg
     1861 gcttcactca ttgcctcacg tcatgaggtt tatggtgttt atcctcatgc actgatatcc
     1921 agcagaaggg ttattcctga cggtgtacag gactcatgga ttagagcaac tgaaagcatt
     1981 atgtcaaatg tttttcttgc accaggagaa gagaaaatca ttaatatatc gggaggccag
     2041 aaggggatat cttccgcatc ggtctggtcc gaactgcttt cccgtatggg ccggaataat
     2101 gagcgattaa ttgttgcggc agtgggtaat gatggcgcag atatacgaaa a
//
LOCUS       DAZGBC010000352         2140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGBC010000352 DAZGBC010000000
VERSION     DAZGBC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.354"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..106)
                     /locus_tag="ACHK1J_005177"
                     /pseudo
     CDS             complement(1..106)
                     /locus_tag="ACHK1J_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            600..842
                     /locus_tag="ACHK1J_005178"
     CDS             600..842
                     /locus_tag="ACHK1J_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914300.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            1133..1501
                     /locus_tag="ACHK1J_005179"
     CDS             1133..1501
                     /locus_tag="ACHK1J_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914301.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            1505..1717
                     /locus_tag="ACHK1J_005180"
     CDS             1505..1717
                     /locus_tag="ACHK1J_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914302.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            1714..1986
                     /locus_tag="ACHK1J_005181"
     CDS             1714..1986
                     /locus_tag="ACHK1J_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFO7914303.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
BASE COUNT          560 a          581 c          504 g          495 t
ORIGIN      
        1 gcggttggca ggccaggtgt tgattacact gatcacgtgg cgtagccacg tttccggctc
       61 gattccgtta agttttgcag ctaccgatca ggctgtacat cactgccgca ctatcgctcg
      121 tcatctcaaa gtccggtctc gtcagcagga aggtatcatt ctctcccgcc atttttccgg
      181 gggcccggtc agataagtcc ctttgtctat cgctgactcc tggctcataa cccggttagc
      241 agaatgcagg gtcaccacct cgccacgacc gaatccggat aagtcaattg caccttctca
      301 atcgccattt tgtcacaatt aagtttgata aagcaggtca cctctgcact gaatctgctc
      361 cagtgcctca ttggtctccg tccccccctt taaactgccg gataagactg ctgcacagcg
      421 ccttccccgg aatcaaatca ctttccctgt ccgccacaat ccgcttcatg ctacctcgta
      481 ttttgtttca gagaccggaa tctgccagaa agtgactacg cgtgcacata tcggttttct
      541 gggttacatg catcgtgtta ttttctgaaa tgcaaaataa taaaaaacaa ggacacataa
      601 tgaaggatga aatacacagt accagcatca acccggatgt tgttgagcag tttctggccc
      661 ttatgaacac acattgtaaa tcctcagaag aggcgctcgc catgttgagt acgctttgtt
      721 acaacctgat ggtgacatgt ggcatccatt ccatggacag ccagatgctc aatgggcatg
      781 ggatatccat gcaacttcag gggattgata ttgccgaaag gccaggtaag aatattcact
      841 aacctgaaca gggcctgaaa acgctgtctt ttcaggcctc tgcaggcaaa aactgatgac
      901 aacaaaattt ctgcgtttgt accccaccga tgaaaaagac aaaaactcac accgggcttc
      961 tagccagtaa agataaaacc cgacgtgtca gcctctacga aacgccaacg gcatggtgta
     1021 tcagggggca ggagtgctac agcaaaagta caggacgccg ctgtggcagc cacgacagtc
     1081 tgagcagact gcgcctagac agcattaagc ctgttgaatg agtggtggca ccatgaaaga
     1141 taacaacaca accgccaccc cagaagcctg gaaaatgcgt tttctggcca acgggacatc
     1201 cggcacatgg ttttacactg acgacattgc agtcactgac gcgtggcttc gcgcgcaccc
     1261 ggatgatgtg gaggttatcc ctctctgccg gataccaccc cgtcagcatt cccatgaaca
     1321 acatattgaa gaactccgcg cagccctggc ccggatgctg catatgcatc atatgatgct
     1381 ggaaaccacg cattacaggg aaaacatcac cgatgcccgt tgtatccgtg aaatgcatga
     1441 agctccatta caggcgctca atattctggc cagatacccg cataaaaagg acgaggtgtg
     1501 acgcatgtgg ctactgattt tctggctttc atctcccttt aatgtctctt ccggtgatgc
     1561 ccggcagccc acactggcgg tgcacagcca gacatttcat accgaacggg cctgtcgcga
     1621 tgcattgcgc gccattaaaa aggagaatgt tggcgaattt tctctgcggg gcgtttgtgt
     1681 ttctgacgga aacacgaagg aggcagagag ctcatgacgg atattactga cagacataca
     1741 cttcgccgca tgtcctggtc ggaacttacc acggcagcgc aggaagcaga attaaaccgt
     1801 gattacgagc gtgccagaat agtgtggtca ttcgccttac atgttgcaac aaccacgatt
     1861 aacaaaaatc tggccatagc tcatattcga cgctgcgata ctttgttgca taaatcaaaa
     1921 acagtacccg gaaacaaccc gagaggccgg tcagtgtgcc tgagacctca acatccccgg
     1981 agatgactga cattccggac tgacgcagtt gctgcatatc tgtggcaata cgttgtatcc
     2041 agccagacag gctatcccgc gtagtgacaa ttaacccgct ttcctgcacc agcttcagcc
     2101 gggccgtcgg ggattttacc agccggaaat atcagtaaaa
//
LOCUS       DAZGBC010000353         2137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGBC010000353 DAZGBC010000000
VERSION     DAZGBC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.355"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1069)
                     /locus_tag="ACHK1J_005182"
     CDS             complement(<1..1069)
                     /locus_tag="ACHK1J_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914304.1"
                     /translation="MGLFNYKDYHQAQITDLISASYNIAVYANTGKVLGLGSFLVSRL
                     FAKHPDRIDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPALGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAVSNG
                     LSGEYVIVTGYSLGGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIGF
                     ENDPVFRVLGDGAGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGAQFYDYMSPDSTVIVDEQSSWTSWYSWVHDKG
                     DESRERPVFIIGNDGNNLLAGG"
     gene            complement(1128..>2137)
                     /locus_tag="ACHK1J_005183"
     CDS             complement(1128..>2137)
                     /locus_tag="ACHK1J_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914305.1"
                     /translation="EFYRQALTSNVSGDLYPQPSIFGDKVSSVSKNWSTLLDSNPRSY
                     VTSQRLDSGVNQYNYNGHTGSDVISITDSLGGLDRTQASRFPVGLFTGEGNDLIVTGR
                     DYGRNTSAGYTDHSHRTDMGNGDDTLVVGVGNNDVTLYVNEEGQLRATTDSYNGSTSI
                     DYTGLNSSSSGGTISGTDIVMGAGNDTVLALGYEGNSADAIINTNIDLGAGNDFIYAN
                     GEISTNNGTQVNIIGGEGFDTISLDNTTVTSAMFSGFEHVDLHSTSHLILNSDDFKSQ
                     DIEGGILKISGSSGASVDVQNFDWENLGSANDGDVKYFTYQSSDISGLTLWIQEGIEV
                     K"
BASE COUNT          651 a          518 c          361 g          607 t
ORIGIN      
        1 tcccgcctgc cagcagatta ttgccatcat taccgatgat gaatacaggt ctttcacgac
       61 tctcatcccc cttgtcatga acccatgaat accaggatgt ccagctactt tgctcatcca
      121 caatgactgt actgtccggt gacatgtagt cgtaaaactg agcccctaaa attctttcaa
      181 aggcgtcagt ttttgaaccc gcaaaatgcg cactccagct tcccagcggg agtgcaagca
      241 cagaaaaggg gataatattc cagaggaatg aaccataaag gtctgtgtat aaaacaatgt
      301 tatcaggaga agagtcatat tcacgatccg gggttgtcaa gcccggcccc attgctttaa
      361 ctgcctccca gaacccggct ccatctccca gcacacgaaa taccggatcg ttttcaaaac
      421 cgatatttaa taccttccca tccccgtcaa ataccagcgg ggaagcccag gctatatagt
      481 cagaattagc ataaaaacca tcagccagag actcactgag tctggccata atgttagtca
      541 acccgccccc caggctatag cctgtgacaa taacatattc accacttaaa ccattactga
      601 ctgcaaagtc tctgatactt tgaagcaggt tttctgcttc agacagcgct tttccattat
      661 tgagctgtgt ataatcaaca atatcgcaga tatcatttgt tccacagaat gatattgcaa
      721 ttttctgtag attctgttga gcatcaaaca acccaacaac tttcaactga ggaccaaatc
      781 ctagcgcagg gtttcctgta accggactgg gaaagcgata aaaacctctt gaatcgacat
      841 attccgaaga taacctgagt tcttccactg taatatcacg ccagccttca ttaagaacat
      901 cggtagaata atcaatccta tctgggtgct ttgcaaatag cctggatacc agaaaacttc
      961 ctaatccaag aacctttcct gtattggcat atactgcaat attataagac gcagagatca
     1021 ggtctgttat ttgcgcttga tgataatctt tataattaaa gagtcccatg agtatttcct
     1081 tatttaaaga acagccccat gctggggctg tttgtttaga ttttctttta tttaacctca
     1141 ataccctcct gtatccaaag agttaaacca gaaatatcac tggactgata ggtgaaatat
     1201 ttaacatcac catcattggc actacctaaa ttctcccagt cgaagttttg tacatccacg
     1261 gatgcacctg aactacctga gatttttaat atccccccct ctatatcctg agatttaaaa
     1321 tcatctgaat tcagaatgag atgagatgta gagtgcaaat caacatgctc aaaaccagag
     1381 aacatagcac ttgttactgt agtattgtcc aaagatatcg tgtcaaagcc ctctccacct
     1441 ataatattta cctgagtacc attgttagtt gaaatctctc cattagcgta aatgaagtca
     1501 ttacctgccc ccaagtctat attcgtatta ataatagcat ccgcactatt accctcatac
     1561 cctaaagcca gaacagtatc atttcctgcc cccataacaa tatcagttcc tgatattgtt
     1621 ccacctgatg atgaagaatt gagcccagta tagtcaatgg atgtactacc attataacta
     1681 tcagtagttg ctctcagttg tccttcttca ttcacatata gtgttacatc gttatttccg
     1741 acaccaacaa ccaaggtatc atctccattc cccatatcag tacgatgaga gtgatccgtg
     1801 tatcctgcac tggtattacg tccataatct cttcctgtaa cgattaaatc attcccttct
     1861 cctgtgaata aaccaaccgg gaagcgagat gcttgagttc tatctaatcc tcctaaagaa
     1921 tcggtaattg agataacatc agaacctgta tgaccattgt aattatattg attaactcct
     1981 gaatctaatc tctgggatgt aacataactt ctaggattac tatcaagtaa agtagaccag
     2041 ttcttagaaa cagaagatac cttatcacca aatatggacg gttgaggata taagtctcct
     2101 gatacattag aggtcaatgc ctgccggtaa aactcca
//
LOCUS       DAZGBC010000354         2133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGBC010000354 DAZGBC010000000
VERSION     DAZGBC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.356"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1733
                     /locus_tag="ACHK1J_005184"
     CDS             <1..1733
                     /locus_tag="ACHK1J_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFO7914306.1"
                     /translation="EDDIEVINFMNGGKSRSEVIQSGDKSRPQSNTWNPFAFSTEAFT
                     AETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSLQMLREHMTLEGMA
                     KLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSET
                     FTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFITQQSM
                     ILARDLGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYTDRIAVEATQVRSLEFSL
                     IMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGS
                     LQRHDGVMGESWVDGNQITIQMESKINVQDLIRLNAGEFFTVFQGDVVPSASVYIPDS
                     EKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSIIGVLTEKTSRKR
                     RKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREER
                     RVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSG
                     GKQESRKKRF"
BASE COUNT          610 a          560 c          525 g          438 t
ORIGIN      
        1 gggaggatga tatcgaagtc atcaacttca tgaacggcgg aaaatcccgc agtgaagtca
       61 ttcagagtgg cgataaatcc cgcccacagt caaacacctg gaacccgttc gcattcagca
      121 cagaagcctt caccgccgaa accatgcagt ccatgcttcc ccagaacgtt caggggggag
      181 aatggcagtc acgggcgata gccatgaaca aagccctggt ctttggcaca aaattctggt
      241 gcgtccggga aagaaaaacc atgtccctgc agatgttacg ggagcatatg acgctggaag
      301 ggatggcaaa actctattgc cgtgggcttg atgatcagtg gccggaagaa gccatcgcac
      361 cactgagaaa ttacctgcag gacgttccgg gattcgatat gtcactggtg agaacgccgt
      421 cagcctggac tgaggagccc cgtaaacagc atgcttatct ctccggtcag ttctcggaaa
      481 cattcacaac ctttgccgaa accttcgggg acgtctttgc ggcagatgcc ggcgacatcg
      541 atattcgcga cagtatccac agcgaccgca ttctgattgt gatgatccct gcactggaca
      601 catcggccca taccacgtcg gcactcggtc gtatgttcat cacccagcag agcatgatcc
      661 tcgcccgtga cctgggctac cgcctggaag gcacagacgc acagaccctg gaagtcaaaa
      721 aatacaaagg ccgcttcccc tatatctgta tccttgacga agtcggtgcc tactacacgg
      781 accgcattgc agtggaggcc acccaggttc gttcactgga gttctccctg ataatgacgg
      841 cgcaggatca ggagcgaatc gaagggcaga cttccgccac caacaccgcc acgctgatgc
      901 agaacgccgg caccaaattc gccggacgca ttgtcagtga cgacaaaaca gcaaggacgg
      961 tgaaaaatgc cgccggtgaa gaggcccgcg ccagaatggg cagcctgcag cgccacgatg
     1021 gtgtgatggg tgagtcctgg gtggacggta accagatcac aatccagatg gaaagcaaaa
     1081 tcaacgtgca ggatttgatc agactgaatg ccggtgagtt ctttaccgtt ttccaggggg
     1141 atgtggtgcc gtcagcctct gtgtatattc cggacagcga gaaaagttgc gacagtgatc
     1201 cagtggtcat caaccgttat atctccgttg aagccccccg actggagcgt ctccgccgtc
     1261 ttgtccccag gacagtgcag cgcaggctcc ccacaccgga gcatgtcagc agcattatcg
     1321 gtgtgctgac agaaaaaacc tcccggaaac gtcgtaaaaa acacacagag ccttaccgaa
     1381 tcatcgatac cttccaggac cagcttaaaa gaacacaaac atcgctggat ctgcttccgc
     1441 aatatgatac agatatcgaa tcccgggcaa acgaactgtg gaaaaaggct gtgcacacta
     1501 tcaataacac aaccagggaa gaacgccggg tatgttatat cacactgaat cgtccggaag
     1561 aggaacactc tggtcctgaa gatatacctt cagtgaaggc gattctgaat actctgctcc
     1621 ccctggagat gctgttacct gtaccggatc taactgcatc cccgccccat aaaaaaaatg
     1681 tcgctcagac accatcaggt ggaaaacaag agagtcgtaa aaaaagattc tgataacacc
     1741 tgtatggctg attttaataa caacctttac gcgattatta atctcctccg ctcaccatag
     1801 taagcggagg attcaggatt caatccatca ccagaggtac aataaatgtg tattagtagc
     1861 ccggaaacaa actcatggtc tgttatttat cgtaaaaatt ccggagaaga tatcaatatc
     1921 acttccctga cgtttaaaaa tagtcttctg gcagcccgga cactgatggt gcccgaaaac
     1981 tatatgatat gcatactcag aaacggggag agggtcagat gatgggacag agaaatactt
     2041 gcaggctcaa acagatggta caagtgttct cccgataatt tcgaaatact gggtaagctc
     2101 cccataataa ataaagtcac aacactgata aaa
//
LOCUS       DAZGBC010000355         2130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGBC010000355 DAZGBC010000000
VERSION     DAZGBC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.357"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            788..>2130
                     /gene="ireA"
                     /locus_tag="ACHK1J_005185"
     CDS             788..>2130
                     /gene="ireA"
                     /locus_tag="ACHK1J_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO7914307.1"
                     /translation="MKNKYIIAPGIAVICSTVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIR"
BASE COUNT          627 a          439 c          489 g          575 t
ORIGIN      
        1 caacatgtta ttgttgttca tgctggcctc cgtggtggaa attccagggg ggtagcaaca
       61 gctcacccat gcggctctga caacgatgtt aacccgcatt aaatatccca cgttggacag
      121 catctgtctc acggaaagtc atactgcctt acgtgcatat ttcaccttac gccttcactg
      181 tctgtcatcg gaagtactga tttatattgg ggctttgcac ttactgtcat tcacgggaaa
      241 agatggtggt gaacagtacg atactgtctt ggctgttgcc gagggaagat tcaggagtgg
      301 taagggggtt agtggtgtaa gatgagtctg aagctctgac tggtcagtga cgcgaaactc
      361 aatctaccag ggtaaactcc cccttgcttt tgcgcagaag cagtgggcct tggttcgctt
      421 atgtcctgca tctacaagcg aatgactgac cggagactcc catatctggt cagtcgccca
      481 tttttatttt ccaccacatc ggacaagcat accgtaataa acagcaccag tctcctgttc
      541 actcttgctt gatttactgg aatgctgttt atggagatat ccggttgaaa gattttacct
      601 cttcgacagg actataaatt atacagtaag gagtccctcg tagaaagggg ctgattctca
      661 tcaagtcgga caggttcaaa gtgttacctg aatgagctaa atgttattta ccttcctttc
      721 tgttcattgt aaattttttc ttgctaacgc aaatcattat cattacgatt ttagaaaagg
      781 gaaatttatg aagaacaaat atatcattgc tccgggcatt gccgtgatat gttctacagt
      841 catatcatca ggttatgcca gttctgataa aaaagaagat acgcttgttg ttactgcctc
      901 cggcttcact cagcaactca gaaatgcacc ggccagtgtt tcagtcatta cttcagagca
      961 actgcaaaaa aaaccggttt cagacctggt cgatgcggta aaagatgttg aagggattag
     1021 tatcaccggt gggaatgaaa aaccggatat cagtatacgt gggttaagtg gcgattatac
     1081 attgattctg gtcgatggac gacgtcagag tggtcgggaa tccagaccaa acggcagcgg
     1141 cggttttgaa gccggattta tccctcctgt ggaagcaatt gagcgtattg aagttatacg
     1201 tggccctatg tcttccctgt atggttccga tgctatcgga ggagttatta atatcataac
     1261 caaacctgtt aataaccaga catgggatgg cgtacttggg cttgggggaa ttattcagga
     1321 acatgggaaa tttggtaact caaccacgaa tgacttctac ctgtcaggcc cattggttaa
     1381 ggataaactt ggtcttcagt tatatggagg aatgaactat cgcaaggaag atagtatctc
     1441 tcagggaaca ccagcaaaag ataataagaa tataacggca acactccagt ttactccgac
     1501 tgaaagccag aagtttgttt ttgaatacgg aaaaaataac caggaacata cattaacacc
     1561 tggtgattct cttgatgcct ggactatgcg gggaaatctt aaacaaccaa acagtaaaag
     1621 agaaacacat aactcacgta gtcactgggt agcagcatgg aatgcccagg gcgaaatact
     1681 gcatcctgaa attgctgttt atcaggagaa agttattcgt gaggttaaat ctggtaaaaa
     1741 agataaatat aatcattggg atcttaatta cgagtcaaga aaaccggaaa taaccaacac
     1801 gatcatagat gcaaaagtga cggcatttct gccggaaaat gtgctgacca tcggaggtca
     1861 atttcagcat gcagaactcc gtgatgactc agccacaggt aaaaaaacga cagaaacaca
     1921 gtctgtttca gttaaacaga aagctgtttt tatagaaaat gaatatgctg caacggattc
     1981 tctcgccctg actggagggt tgcgcctcga taaccatgaa atctatggca gttactggaa
     2041 tccaagattg tatgctgttt ataacctgac cgataatctc actctcaaag gagggatcgc
     2101 aaaagcattt cgggcccctt cgattcgtga
//
LOCUS       DAZGBC010000356         2111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGBC010000356 DAZGBC010000000
VERSION     DAZGBC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.358"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHK1J_005186"
     CDS             <1..207
                     /locus_tag="ACHK1J_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914308.1"
                     /translation="HLENWMNGGLTIESVKETLIKKGWSNKMLAARWGCSEVWVSKII
                     NDENRKVQWNDAINGLPVISDNMV"
     gene            356..>2111
                     /locus_tag="ACHK1J_005187"
     CDS             356..>2111
                     /locus_tag="ACHK1J_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914309.1"
                     /translation="MEKVTTKKINRTEHFPSLTLPSDTDWVVWWYGSLKKHKRRGEQP
                     KITVVFREIQTDGTLCDRYYEQECLLTDLSFFKIGSVWARGRCIAEILMEEKVFNVDF
                     SYANGGWKINSLYHAYQEGVSLPYDFSDYKLKSPYDKNWLLEFSTDGTAKIIIHPLEF
                     FYRCYGHSDELKRVILTYSGNELRNRIFSEAYVEGDKIFYIKLRKRMNKYDAPFASFF
                     ALDGFTQKVIERIQRQLDVCEDRDIYLKVAPWFKGKARIKVKGFWLGNNITFLGLSVT
                     GVSLPAGYTINYDRDNSNLVDEKAEQGSRQAWSNVPVRHIKNPDEPFSVDNAFSPDSG
                     ASTLEVEASVFEFLDDGCVLSPLRRKKAKYTSATKNESDLHSLYSVNEVVDEHNSIST
                     GYMSLADKNEPERLYMESKGIVRDMWNAMVYLREAYSERVKSLDWYTPENSFLNTQEP
                     ELIAIPEFNESEAENDKISRSIINWPYLDAVNKKDIRGFLISRMLIDDNVIYFMEIQR
                     RVCNEETMNSVSEVEKFKGLVFTLDNECEIDKWVSLLAHESRFVKGILQKIVGKCPGT
                     AMTYKHSPAKNEPVACYSA"
BASE COUNT          693 a          286 c          473 g          659 t
ORIGIN      
        1 catcttgaaa attggatgaa tggaggtctt accattgaaa gtgtaaaaga aacgcttata
       61 aaaaaaggtt ggagtaataa aatgttagct gcgcgttggg gatgttcaga agtatgggtg
      121 agtaaaatta ttaatgatga aaatcgaaaa gttcagtgga atgatgctat taatgggctt
      181 cctgttataa gtgataatat ggtttgattt gcagttgtgt tttgcctgca taaggcgaga
      241 aaattactaa taattcattc tggatgcagt tcgtatcaga acgttttttg aagtgaataa
      301 ttgttttaat gtctgcttgt aatataagaa ctatattgta ttgaggagta gatgtatgga
      361 aaaagtaaca acgaaaaaaa taaacaggac agaacatttc ccttcattaa cgcttccgtc
      421 tgatacagac tgggttgttt ggtggtatgg ttctttaaaa aaacataaaa gacgaggtga
      481 gcaacctaaa ataacagttg tctttcggga gattcaaacc gatggaactc tttgtgacag
      541 atattatgag caggaatgtc tgttgactga tttgtctttt tttaaaatag gcagtgtctg
      601 ggcaagagga cggtgtattg ctgaaattct gatggaggaa aaagtattta atgtggattt
      661 ctcttatgca aatggtgggt ggaagattaa ttctctttac catgcatatc aagaaggtgt
      721 gtcattacct tatgattttt cagattataa actgaagtct ccatatgata aaaactggct
      781 ccttgaattt tcaacagatg gtacggctaa gataatcatc catccgcttg agttttttta
      841 tcgttgctat ggacattctg atgaactgaa gagggttatt cttacttatt caggaaatga
      901 actacgtaac aggatttttt ctgaggcata cgtggaggga gacaagattt tttatataaa
      961 attaagaaag agaatgaata aatatgatgc tccctttgca tctttctttg cccttgatgg
     1021 gtttacccag aaagtgattg aaagaataca acgtcaatta gatgtatgtg aagatcgtga
     1081 catatacctc aaagtagctc cctggtttaa aggaaaggcc agaattaaag tcaaaggttt
     1141 ctggctggga aataatatta ccttcctggg gctttctgta acaggtgttt ctttgccagc
     1201 aggatatact ataaattatg acagagataa cagtaatctg gttgatgaaa aagctgaaca
     1261 agggagcagg caagcctgga gtaatgttcc cgttcggcat ataaaaaatc cggatgaacc
     1321 tttcagtgtt gataacgctt tttcgcctga ttctggagcg tcgactcttg aagttgaagc
     1381 gtcagttttt gagtttcttg acgatggctg tgtattgagt cctttaagaa gaaaaaaagc
     1441 aaaatataca tctgctacaa aaaatgaaag tgatttacat tcattatatt cagtaaatga
     1501 agttgttgat gaacacaata gcataagcac aggatatatg tcattagctg ataaaaatga
     1561 acccgaaaga ttatatatgg agtcaaaagg aattgttcgt gacatgtgga atgctatggt
     1621 ttatttgagg gaagcttatt ctgagagggt taagtctctg gactggtata caccagaaaa
     1681 ctcattctta aatacgcaag aacctgaact tattgcaatt ccagagttca atgaaagcga
     1741 ggcggaaaat gataaaattt ccaggtcgat tattaactgg ccttatcttg atgctgtgaa
     1801 taagaaagat ataagaggtt ttttgatatc aaggatgttg attgatgata atgtgattta
     1861 ttttatggaa atacagagga gagtgtgcaa tgaagaaact atgaattcgg tctctgaagt
     1921 ggaaaaattt aaaggtctgg tttttacact tgataatgaa tgtgaaatcg ataaatgggt
     1981 atctttgttg gctcacgaat caagatttgt taaaggtatt ttacaaaaaa tagtggggaa
     2041 atgcccgggc acagcgatga cttacaagca ttctcccgca aaaaatgaac ccgttgcttg
     2101 ttatagcgct t
//
LOCUS       DAZGBC010000357         2092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGBC010000357 DAZGBC010000000
VERSION     DAZGBC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.359"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..301
                     /locus_tag="ACHK1J_005188"
     CDS             <1..301
                     /locus_tag="ACHK1J_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914310.1"
                     /translation="KITISENGKVTPPSHQHSEELIEFAIDYLKNNKKQGLMQRIGRC
                     MGYLQVAAEIEALASGADKDSVVREALLRDFDNPPFKKVPAYWLHPGLTYLKVRI"
     gene            338..871
                     /locus_tag="ACHK1J_005189"
     CDS             338..871
                     /locus_tag="ACHK1J_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7914311.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            1028..1210
                     /locus_tag="ACHK1J_005190"
     CDS             1028..1210
                     /locus_tag="ACHK1J_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914312.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1225..1356
                     /locus_tag="ACHK1J_005191"
     CDS             1225..1356
                     /locus_tag="ACHK1J_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914313.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            1579..1764
                     /locus_tag="ACHK1J_005192"
     CDS             1579..1764
                     /locus_tag="ACHK1J_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7914314.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPPVQAPCVKPPPPPAWIMQPA
                     PDWQTQLNGIISPSESG"
BASE COUNT          525 a          482 c          596 g          489 t
ORIGIN      
        1 gaaaataacc atctcagaaa atgggaaagt aacacctcca tcgcaccagc acagtgagga
       61 gctcattgaa tttgccattg attacctgaa gaacaataaa aaacaggggc tgatgcagcg
      121 cattggtcgt tgcatggggt atctgcaggt agccgctgag attgaagcgc tggccagtgg
      181 tgcggacaag gattcagttg tgcgggaggc acttcttcgt gattttgata atccgccctt
      241 taaaaaagtg ccggcttact ggcttcatcc cggactgact tatctgaaag tgcgtattta
      301 gtgggcctgg gacagcggct gaatatttaa tatatccatg aacacaaaaa tcaaatacgg
      361 cctgtcggct gccgttctgg cgttgattgc cgcaggggct tctgcgcctg aaatcctcga
      421 ccagtttctg gatgaaaaag aaggtaacca caccacggca taccgtgatg gcgcgggtat
      481 ctggaccatc tgccgtggag ccacccgggt ggatggtaag cctgttattc ctggcatgaa
      541 gctgtcgaag gggaaatgcg accaggttaa cgccattgag cgtgataagg cgctggcatg
      601 ggtggagaaa aacatcagag tgccactgac tgaaccccag aaagcgggta ttgcgtcatt
      661 ctgtccttac aacattggcc cgggtaagtg tttcccgtcg acgttttata aacgaattaa
      721 tgcaggcgat cgcaggggag cgtgtgaagc gattcgctgg tggattaagg acggtggcag
      781 agactgccgt atccgctcaa acaactgtta tggtcagata tcccgtcgtg accaggagag
      841 cgcgctggcg tgctggggaa tcgacagata agcagaatat tttgctgaaa aatgacgttg
      901 gccaacgcgg acggataaca cgaaatcctg cgaactggca aaacctaagt gaataaaagt
      961 aaaaaccccg tttgttggca gcaagcgggg ttttgtgttt ctgaccttgg ataaggcaag
     1021 ggagaacatg gaaaagtata aacgaattct gttgaggttg actatgaaaa acggccttga
     1081 actgaaagcg ccagtaactg atgacatcag cagagcactg gcttttgcta ttaagtgggt
     1141 ggcggtcggt atcgctgtgt ctccgatgct gtatgggctg gcaaaactgg tcattgcgtt
     1201 gaaatcgtga agggaggatt aagcatgtca gacaaactca taatgctggc gaagatcctc
     1261 tgtgtaatcg tcggtatttc attttcacta atgctggttg ctatttttct tttcctgccc
     1321 tggatgatgt tgtcttcgtc ggggatgctg gggtgagcat aaaccgaatg ctttccgcgt
     1381 ttaccgttat tctgctggtg gcctgtggtg cgcttagtct ggggctgaat cattaccgcg
     1441 ataacgccat cacctacaaa gcgcagcgcg ataaaaaagc cagagagctg gaacaggcga
     1501 atgccaccat tactgacatg caggtgcgcc agcgtgatgt tgctgcgctc gatgcaaaat
     1561 actcgaggga attagccgat gcgagagctg aaaatgaaac tctgcgcgct gatgttgccg
     1621 ctggtcgtaa gcgcctgcgg atcaacgcca cctgtccagg ctccgtgcgt gaagccccca
     1681 ccacctccgg cgtggataat gcaaccggcc cccgactggc agacacagtt gaacgggatt
     1741 atttcaccct cagagagcgg ttgatgatga tgcagaagca gctggaaggg gcgcaggaat
     1801 atatccgcac tcagtgcctg aaataagttt tgctgatgcg ccgtatcgtc gccgtattcc
     1861 cgtattaaca gagaccgcag cccgacaggg agactcctct gcgagagtgt gcggggataa
     1921 tcaaaaacga tacacaccgg ggtttaccgc gttaacagag cgcggcgttg tcccctcata
     1981 gtcgcctgtc cggtgcgatg gtggaagaaa ctggatttgt tgcaactgat aaccattatc
     2041 attttctcgg gtcctttccg gcatatggac ccgttacggg gcggcgacct cg
//
LOCUS       DAZGBC010000358         2078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGBC010000358 DAZGBC010000000
VERSION     DAZGBC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.360"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..83)
                     /gene="ylcJ"
                     /locus_tag="ACHK1J_005193"
                     /pseudo
     CDS             complement(1..83)
                     /gene="ylcJ"
                     /locus_tag="ACHK1J_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518754.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
     gene            complement(132..464)
                     /gene="emrE"
                     /locus_tag="ACHK1J_005194"
     CDS             complement(132..464)
                     /gene="emrE"
                     /locus_tag="ACHK1J_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO7914315.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(532..834)
                     /locus_tag="ACHK1J_005195"
     CDS             complement(532..834)
                     /locus_tag="ACHK1J_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO7914316.1"
                     /translation="MTGKEAIIHYLGTHKKFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(831..1532)
                     /locus_tag="ACHK1J_005196"
     CDS             complement(831..1532)
                     /locus_tag="ACHK1J_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7914317.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGIATMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGARV"
     gene            complement(1529..>2078)
                     /locus_tag="ACHK1J_005197"
     CDS             complement(1529..>2078)
                     /locus_tag="ACHK1J_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO7914318.1"
                     /translation="YLKEVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSE
                     NSDESSDQPENDLPVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAG
                     WANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQA
                     GVTASKPKLDLTNTDWIYGVDL"
BASE COUNT          499 a          548 c          452 g          579 t
ORIGIN      
        1 tttcttacgc cataatcttg aaagcacaaa agaatacata aaaaaaataa agatattaac
       61 aaaaagcata aaacgaggct catataaata tacgagcctc catattttag tcgtttagaa
      121 acaaattact tttaatgtgg tgtgcttcgt gacaataaat taataaccaa cacaccggca
      181 caaatcaaca tcatgcctat aatggctggc aggtccagcc gttggccgaa aaacccccat
      241 gacagtaagc taatcaggac aataccgact cctgaccaga tagcataagc aatccctgta
      301 ggaatataag ccagcgtctg agctaataac cagaatgatg cacaataaca aataattgta
      361 ccaacagatg gccataaccg tgtaaaacct tctgaaaact tcattaaggt tgtaccaatg
      421 acctctgcaa gtattgcacc accaagataa atataagggt tcatagcata ttctttcctg
      481 ttcaaactgg agagaattgt actacagttt gaactcaact cacctgtttc atcattgtgt
      541 acccattgat gttcttttat ataccctcaa tacccgtttc atcgcggcac tctggcgaca
      601 ctccttaaaa atcagattcg tgctcacctt tccttcccgt tcttctctgg tagcgaaccg
      661 gtaatacacc gttcgccaga cctttccatc aacgaccagg attcctgccc gcgccatttt
      721 agccgcagcc tgatttatgc tggttaccgt tgcgcctgtt accgcggcaa cgtcctgcgc
      781 acagaatttc ttatgagtcc ccaggtaatg aataattgcc tctttgcccg tcataccctt
      841 gctcctttca gcccaaactt agctttgatt tctgcgatct tcgccagagc ctgtgcacga
      901 tttagaggtc taccgcccat gacaggaagt tgttttactg gttcaggtat agcctcacca
      961 cggttaattc gtgcggtcat acaggacagt tcatcggcag ccttgcgccg taattccgcg
     1021 tcagtcaacg cattggcccg catgttctgg tacaagttgg taaccaacca gtaatgcgcg
     1081 ttcgatttcc acggataaga ctctgcatcc ggatacaggc cacgcttccg gcaatactcg
     1141 taaaccatat caaccagctc gctgacgttt ggcagcccgg cgttaacgga tgcttcttcc
     1201 cggcaccagg cgacaaactg cccgggtgat ggcaggaatg gtcgattctg ccgacgggct
     1261 acgcgcattc ctgcgttaac ctgttccatt gtggcgatcc cgttttcccg gaaagccagc
     1321 acccactggc ggcggatttc gttcagttcg ttctggtccc ggttagccag gctcgccggg
     1381 aaagttgcca gtaactggct gaacacacca ttgataatct gcgctacctg ctgtacctgc
     1441 ggcttttcgt cgtactgttc cggcatgttg ttggcgatcc gacgcatctg ctcacggtca
     1501 aagttaatca tctgtgcggc gatgtttttc atagatcaac cccgtaaatc cagtctgtgt
     1561 ttgtcaggtc gagttttggt ttgctggctg tcacgcctgc ctgttgcttg ttacggttga
     1621 tttcgagctg ggtccacttg tcgcggagtt tggccggact tagcacgtta ccggaccaga
     1681 agttgtcctg gcatgcccag cggaacagca cgcacatgtc gcggtggtta cgtccatcac
     1741 gttcacgcat caggcggata tcgttagccc accctgcaaa attcggtttt ctggctgatg
     1801 gcgcgatggt cttcaccatg tcaaacatcc actctgcggc ggtcaggtct tctgctgtcc
     1861 cccacttgct gccgctctga attgcagcat ccggtttcac cacaggaaga tcgttttctg
     1921 gctggtcaga ggattcgtca gaattctcgg acgaaaaagg ttttatattg tcttttgtta
     1981 gtttgtcttt tgtgtttacc tgattcgggt aaacgccttt acctgatttg ggtaaacttt
     2041 tcttacctga ttcaggtaaa tttacctctt tcaggtaa
//
LOCUS       DAZGBC010000359         2073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGBC010000359 DAZGBC010000000
VERSION     DAZGBC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.361"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            271..>2073
                     /locus_tag="ACHK1J_005198"
     CDS             271..>2073
                     /locus_tag="ACHK1J_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFO7914319.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVASGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVVMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGY"
BASE COUNT          585 a          471 c          509 g          508 t
ORIGIN      
        1 aatgtttttg tcatattcat agtaacgaat tggaataagt aacgacaact tagggaataa
       61 ctcgatctaa actcaccttt gtaaggggga tgttaaatat atattaattc ctgtgtttct
      121 gattttgccc ggtaatagag aattattctc atagcagctc atcatgattg caccatacaa
      181 atatcatgat gataagagga tattattcat tgacttaaac aaaatgacaa aatccataga
      241 cccatagcct gggtactata taggagtggt atgggcatca aacaacacaa tgggaatacc
      301 aaagccgatc gtctcgctga attaaaaatc cgttcgccct caattcaact gataaaattt
      361 ggcgctattg gtttgaatgc aattatcttt tcccccctgc tgatagctgc tgatacagga
      421 agtcaatatg gcaccaatat tactattaat gatggtgaca gaattacagg agataccgcc
      481 gatccatcag gaaacctcta tggtgtaatg accccagcag gaaacacgcc tggcaatatc
      541 aacctgggta atgatgtcac cgtcaatgtc aacgacgcct ctggatatgc aaaaggaatc
      601 attattcagg gcaaaaacag ctccctgaca gctaaccgac tcacagtaga tgttgttggt
      661 caaacctctg ccatcggcat taatttaatt ggtgactata cccatgctga cttaggcaca
      721 ggcagcacca ttaagagtaa cgatgacggc atcattattg ggcatagctc aacactaaca
      781 gccactcaat tcaccattga aaactcgaac ggtataggcc taaccatcaa tgactatggc
      841 accagtgtcg atcttggaag cggaagtaaa atcaagaccg atggaagtac aggtgtttat
      901 atcggtggtc tcaacggcaa taacgccaat ggtgctgcgc gttttacggc gacagacctg
      961 acaatcgatg ttcagggcta cagcgccatg gggataaacg tacagaaaaa ctctgttgtc
     1021 gatctcggaa caaacagtac cattaaaacc aatggcgata atgctcacgg cctctggagc
     1081 tttggccagg ttagcgcgaa tgcactcact gttgatgtaa ctggagccgc ggccaatggc
     1141 gtcgaagttc gtggtggtac aaccactatc ggtgcagata gccatatttc ttccgcgcag
     1201 ggcggtggtc tcgtagccag tggttcagac gcgacaatca atttttctgg cacggcagcg
     1261 caacgaaaca gcatcttttc cggcggctct tatggtgcct cggcccagac ggcaacggct
     1321 gttatcaaca tgcaaaatac ggatattacg gttgatcgta atggcagtct ggcgctgggt
     1381 ttgtgggcgc tcagcggcgg tagaataacc ggagacagtt tggctatcac cggcgcggca
     1441 ggagccaggg ggatttatgc catgaccaac agccagatcg acctcacgag cgatctggtc
     1501 attgatatga gtacacccga ccagatggcc atcgcaacgc aacatgacga tggttatgcc
     1561 gccagccgca tcaacgcctc gggtcgtatg cttatcaacg gtagcgttct ttccaaaggt
     1621 gggctaatca atctggatat gcaccctggg tcggtttgga caggttcctc cctcagcgat
     1681 aatgtcaatg gcgggaaact ggacgttgta atgaataaca gcgtctggaa cgtaacaagt
     1741 aattctaatc tcgacacgct ggcgctgagc cattcaactg tcgattttgc cagccacggg
     1801 tcaactgccg gcacatttac cacattaaac gtagagaacc tgagcggtaa cagtaccttt
     1861 attatgcgtg ctgatgttgt tggcgagggt aatggcgtta ataataaagg ggatttattg
     1921 aatatcagcg ggagtagtgc tggtaatcat gtattggcta tccgcaacca gggcagcgag
     1981 gccacaacgg gaaatgaagt tctgacagtg gtaaaaacca ctgacggtgc ggcctcgttc
     2041 agcgcgtctt ctcaggttga gttgggggga tat
//
LOCUS       DAZGBC010000360         2032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGBC010000360 DAZGBC010000000
VERSION     DAZGBC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.362"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..96
                     /locus_tag="ACHK1J_005199"
     CDS             <1..96
                     /locus_tag="ACHK1J_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO7914320.1"
                     /translation="VLGSLLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            101..634
                     /locus_tag="ACHK1J_005200"
     CDS             101..634
                     /locus_tag="ACHK1J_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7914321.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCAQINAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIDR"
     gene            855..968
                     /locus_tag="ACHK1J_005201"
     CDS             855..968
                     /locus_tag="ACHK1J_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914322.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            970..1464
                     /locus_tag="ACHK1J_005202"
     CDS             970..1464
                     /locus_tag="ACHK1J_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7914323.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCIKLSFYY
                     PEDI"
     gene            1461..1685
                     /locus_tag="ACHK1J_005203"
     CDS             1461..1685
                     /locus_tag="ACHK1J_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914324.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQPGRVLVEGTFSGKCTQSYSRVFR
                     VDASNEELTVECAVNAAKCRMVSAALQPVC"
BASE COUNT          501 a          446 c          589 g          496 t
ORIGIN      
        1 gtgctgggga gtctgctgtt tgggctgctg acatatctga cgaacctgta tttcaagatt
       61 aaagaagacc ggcgtaaggc ggcgcgggga gagtaagctg atgagcagga aattccgcta
      121 tggtttatca gcggtcgttc tggcgctgat tgctgcaggt gcttctgcgc ctgaaatcct
      181 cgaccagttt cttgacgaaa aagaaggtaa ccacacaaca gcataccgtg atggtgcggg
      241 tatctggacc atctgccgtg gtgccacccg ggtggatggt aagcctgtga ttcctggcat
      301 gaagctgtcg aaggaaaaat gcgcacagat taatgccatt gaacgtgata aggcgctggc
      361 atgggtggcg aaaaacatca gagtgccact gaccgaacct cagaaagcgg gtattgcgtc
      421 attctgtcct tacaacattg gccccggtaa gtgtttcccg tcgacgtttt ataaacgaat
      481 taatgcaggc gatcgcaggg gagcatgtga ggcgattcgc tggtggatta aggacggtgg
      541 cagggactgc cgtattcgct caaataactg ctacgggcag gttatacgtc gtgaccagga
      601 aagcgcgctg acgtgctggg gcatcgacag ataagcagaa tattttgctg aaaaatgacg
      661 ttgaccaacg cgaacggata acacgaaatc ctgcgaactg gcaaaatcta agtgaataaa
      721 agtaaaaacc ccgtttgttg gcagcaagcg gggttttgtg tttctgacct tggataaggc
      781 aagggagaaa ttatggtgag ggaggtgctt tccctgtgag gaagtataaa agattctttc
      841 tgaggttgtc cattatgaaa ggcattgaag tggagacgcc agccagtctg gatttaacaa
      901 gagcggcagc ttttgccatt cgtattatag ccattgctgt tctgatctgg gcaatccgtt
      961 ggtggtgaaa tgaaccgcgt tctgtgtgtg gtcatcattg ctctgctggt ggcctgtggt
     1021 gcgcttagtc tggggctgaa tcattaccgc gataacgcca tcacttacaa agcgcagcgc
     1081 gataaaaaag ccagagagct ggagctggca aacgcaacca ttactgatat gcaggtgcgc
     1141 cagcgtgatg ttgctgcgct cgatgcaaaa tactcgaggg aattagccga tgcgagagct
     1201 gaaaatgaaa ctcttcgcgc tgacgttgcc gctggtcgta agcgcctgcg tatcaacgcc
     1261 acctgtccag gtcccgtgcg tgaagccacc ggcaccgccc gcgtggataa tgcaaccggc
     1321 ccccaactgg cagacaccgt tacacgggat tatttcaccc tcagagagcg gctgatgacg
     1381 atgcagaagc aactggaagg ggcgcaggaa tatatccgta ctcagtgcat taagttgtct
     1441 ttttattatc cggaagatat atgaagaaat tactggtaac cgtaaagcct tttcagggaa
     1501 caattccgtt ccgtgttttg cagcctggtc gtgttcttgt tgaaggtacg ttcagtggta
     1561 aatgtacgca atcatactcc cgggtctttc gggtggatgc ctcgaatgaa gagctaaccg
     1621 tggagtgtgc tgtgaatgcc gctaaatgcc gcatggtatc ggctgcatta cagccagtgt
     1681 gttgagcgac cttattatcc atggcgcggt attgtcgccg tattcccgta ttaacagaga
     1741 ccgcagcccg acagggagac tcctctgcgc gagtgtgcgg ggataatcaa aaacgataca
     1801 caccggggtt taccgcgtta acggagcgcg gcgttgtccc ctcatggtcg ctggtccggt
     1861 gcgatggtgg aagaaaccgg acgatgtgtt acctcgcatg acctgttatg tcatgtgtct
     1921 gatttgtgat ttaagtcgga taattgtcgt tgccattaag cagaggattg atggccgaca
     1981 gggtggcatt gttagaataa gacttattct tatctgtgcc gggaatgaaa aa
//
LOCUS       DAZGBC010000361         2025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGBC010000361 DAZGBC010000000
VERSION     DAZGBC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.363"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            110..370
                     /locus_tag="ACHK1J_005204"
     CDS             110..370
                     /locus_tag="ACHK1J_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO7914325.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            594..668
                     /gene="tap"
                     /locus_tag="ACHK1J_005205"
     CDS             594..668
                     /gene="tap"
                     /locus_tag="ACHK1J_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO7914326.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            661..1518
                     /gene="repA"
                     /locus_tag="ACHK1J_005206"
     CDS             661..1518
                     /gene="repA"
                     /locus_tag="ACHK1J_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7914327.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
BASE COUNT          531 a          529 c          512 g          453 t
ORIGIN      
        1 ttgatttgtg cgccaacaca gatcttcgtc acaattctca agtcgctgat ttcaaaaaac
       61 tgtagtatcc tctgcgaaac gatccctgtt tgagaattga ggaggcgaga tgtcgcagac
      121 agaaaatgca gtgacttcct cattgagtca aaagcggttt gtgcgcagag gtaagcctat
      181 gactgactct gagaaacaaa tggccgctgt tgcaagaaaa cgtcttacac acaaagagat
      241 aaaagttttt gtcaaaaatc ctctgaaaga tctcatggtt gagtactgcg agagagaggg
      301 gataacacag gctcagttcg ttgagaaaat catcaaagat gaactgcaga gactggatat
      361 actaaagtaa agactttact ttgtggcgta gcatgctaga ttactgatcg tttaaggaat
      421 tttgtggctg gccacgccgt aaggtggcag ggaactggtt ctgatgtgga tttacaggag
      481 ccagaaaagt gaaaaccccg ataatcttcg acaagtttgg cgactgagaa gattaccggg
      541 gccatctaaa aaccgtatag ccaacaattc agctatgcgg ggagtatagt tatatgcccg
      601 gaaaagttca agacttcttt ctgtgctcgc tccttctgcg cattgtaagt gcaggatggt
      661 gtgactgatc ttcaacaaac gtattaccgc caggtaaaga acccgaatcc ggtgtttaca
      721 ccccgtaaag gtgccggaac gctgaagttc tgcgaaaaac tgatggaaaa ggcggtgggc
      781 ttcacctccc gttttgattt cgccattcat gtggcgcatg cccgttcgcg tggtctgcgt
      841 cgacgcatgc caccggtgct gcgtcgacgg gctattgatg cgctgctgca ggggctgtgt
      901 ttccattatg acccgctggc caaccgcgtc cagtgttcca ttaccacact ggccattgag
      961 tgcggactgg cgacagagtc cggtgcagga aaactctcca tcacccgggc cacccgggcc
     1021 ctgacgttcc tgtcagagct gggactgatt acctaccaga cggaatatga cccgcttatc
     1081 gggtgctaca ttccgaccga tatcacgttc acaccggcgc tgtttgccgc ccttgatgtg
     1141 tctgaggatg cagtggcagc tgcgcgccgc agccgtgtgg tatgggaaaa cagacagcgc
     1201 aaaaagcagg ggctggatac cctgggtatg gatgagctga tagcgaaagc ctggcgtttt
     1261 gtccgtgagc gtttccgcag ttaccagaca gagcttaagt cccacggcat gaaacgtgcc
     1321 cgtgcgcgcc gcgatgcagg cagaacccgc caggacattg tcaccctggt gaaacggcag
     1381 ctgacgcgtg aaatcgcgga agggcgtttc cggggaagcc tggaggccgt gaagcgcgaa
     1441 atcgaccgtc gtgtgaagga acgcatgata atgtcgcgga acaacaacta cacacggctg
     1501 gccactgcct ccccctgagt tttacctcat ctgaataatc cggcctgcgc cggaggcatc
     1561 tgcacgcctg aagcctgtca gcgaacaaaa aaacagcacc acatacaaaa aacaatctca
     1621 tcatcccgct tctggtgcat ccggcccccc tgttttcgat acaaaacatg cctcacagac
     1681 gtggaatttt gcttatccac atttaactgc aagggacttc cccataaggt tacaaccgtt
     1741 catgtcataa agcgccagcc gtcagcgtta cagggtgcaa tgtatctttt aaacacctgt
     1801 ttatatctcc tttaaactac ttaattacat tcatttaaaa agaaaaccta ttcattgcct
     1861 gtcctgtgga cagacagata tgcacctccc accgcaagcg gcgggccccg accggagcca
     1921 ttttagttac aacactcaaa tacaagcacc gggaaaaccc cggtccagcg cagaaccgaa
     1981 accgcaaagc ccctccctca taacttaaaa gcggccccgc cccgg
//
LOCUS       DAZGBC010000362         2008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGBC010000362 DAZGBC010000000
VERSION     DAZGBC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.364"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..859
                     /gene="nanR"
                     /locus_tag="ACHK1J_005207"
                     /pseudo
     CDS             180..859
                     /gene="nanR"
                     /locus_tag="ACHK1J_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505672.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
     gene            complement(1318..1632)
                     /locus_tag="ACHK1J_005208"
     CDS             complement(1318..1632)
                     /locus_tag="ACHK1J_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914328.1"
                     /translation="MITVIISEVDGWREWKHRARTKDAQTAIIRAMNKHFPRSYNFIP
                     DDIDNAPVLFAAVTRTPNVKITGHIWKPMWNRGICWNVKGPPVIITLIQGAAWNSENK
                     PR"
     gene            complement(1700..>2008)
                     /locus_tag="ACHK1J_005209"
     CDS             complement(1700..>2008)
                     /locus_tag="ACHK1J_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7914329.1"
                     /translation="MAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGL
                     DGKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSTNCHTGQQFEGRLHS
                     "
BASE COUNT          595 a          392 c          411 g          610 t
ORIGIN      
        1 ggaatgatat taataatgat gactgttatt tgtagtttga atttttagcc acgatgaacg
       61 agaatattaa tgcataaact atgctcaatc agatgaatga ttcagtctct gatgtatctg
      121 taaaattata ttttgtttca ttcatgtaac ttaagtgttg atttttaaac tggtaaatta
      181 tgtttgataa acgaccgtta aaaagaaaaa aactctctga gttagttgag gaagaactgg
      241 agtttatgat cagaaaagga atttttcagg agggtgaaca tataccgtca gagcggacac
      301 tgatggaaat gtttaaagtt ggcagaccat ctgttcgtga agccttggct actttacgac
      361 gtaaaggact tattgaagtt aataatggtg aaagaacgaa ggtttgtcga cccactcctt
      421 ttactcttat aaatgagtta tctggccagc gacgagggaa tgcaacattt tgaacaatta
      481 cgactttttt tcgagttaat gttagtcaga tatgcttcgg tttatgcaaa caaggagcaa
      541 aaaaaataaa cttaatcagg ctgcaaacat ttgttggaaa tatgttgatg ataatattaa
      601 ctttattcgt gcagatatta acttccatcg ggttttatct gaaatccctg aaaaccctgt
      661 atttgttgct attcatacag cactaacaga ctggttgata aatacacatt ctgaagagca
      721 cagtaatata acacgtgaac aaaatattac aagcgttaaa cagcatgagg ctatttatga
      781 agctatatgt gaaaaagatg cagataaagc agaaacaata cttaaagaac atctgttaca
      841 tcgtttaaca gccacatagt tttgaagttt aaaattattt gaaaattatc tgcggggttc
      901 tgcagataat gtttttgctc cataacaggc tgaaaaggtg ggcacctacg ttaccaatac
      961 aggcttaacg gctacatacg gcagtcggtt tacgcttact ttttaaccga tgtgaataag
     1021 caactgattg atgaaatggg catcttcacg gtctccatgg cggtacgagg ggtccgcttg
     1081 gaaaacggaa attttcccac gctaagagaa ttgagttgaa cgagtggtta catccaagcc
     1141 agctaactca ctgaacgtaa aggctgtacg aaatgttccg accgtccgat aaccatttgg
     1201 cttacttccg tacatttcct tgcgtagatg tacagaattg tacgatttcg gacaccgaaa
     1261 cagctgttgt cccgccatcc tggcgattcc tgaaagtcat ttgtctaaaa aatagtctta
     1321 acgtggttta ttttccgaat tccaagcggc cccctggatg agcgtgatga ttactggcgg
     1381 acctttgacg ttccagcaaa ttcccctgtt ccacataggt ttccagatat gaccggttat
     1441 tttcacgttc ggtgtacgtg ttacagctgc aaataataca ggtgcattat caatatcatc
     1501 agggataaaa ttgtaagatc gtggaaaatg tttattcata gcccggatga tggctgtctg
     1561 tgcatccttt gttcgggcgc ggtgtttcca ttcacgccag ccatctacct cagaaataat
     1621 aacagtaatc atcaaaacct cgtgtcgtta ctcaattctg attccatagc gggacagcat
     1681 ttcacataat tatgccccgt tatgaatgca ggcgtccttc aaattgctgt ccggtgtgac
     1741 agtttgtcga tggttggttg caacgagcat cttctgtctc tttcatcctg gcgttataac
     1801 gctgcgtgtg tttttcaagt tttgcctgcg catacagcgc ttcttcctcc gttacagttc
     1861 catgaacttt gccatccaga ccataacgcg gagtacctgc gactgatata cgctgcagat
     1921 atttgtaacc gccagtaatc atccttacag cacaattcca ttcttttaca gttagtccac
     1981 actcaggact ggccgaaata aaagccat
//
LOCUS       DAZGBC010000363         1997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGBC010000363 DAZGBC010000000
VERSION     DAZGBC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.365"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..1493)
                     /gene="pykF"
                     /locus_tag="ACHK1J_005210"
     CDS             complement(81..1493)
                     /gene="pykF"
                     /locus_tag="ACHK1J_005210"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO7914330.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1501..1695)
                     /locus_tag="ACHK1J_005211"
     CDS             complement(1501..1695)
                     /locus_tag="ACHK1J_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914331.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          504 a          456 c          481 g          556 t
ORIGIN      
        1 tattgcttct ggttatcgat taaataaaaa aagcgcccat cagggcgctt cgatatacaa
       61 attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt agtgccgctc
      121 ggtaccagtg caccagaaac cataactaca acgtcacctt tgtgtgccag accgctctgc
      181 agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc tttaaccagc
      241 tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt ggtggtcagt
      301 gccaggatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt accgccctgg
      361 gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc accacggcat
      421 accgcttcgg taatgcgcag tttacggttg tcattgttga actcaagacg gctgttcatc
      481 acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag cgggtattta
      541 ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat ggcgtttgca
      601 acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc cagcatctgg
      661 gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat cttctgggcg
      721 aagataactt cttctaccgg gatttctaca cccaggtcgc cacgcgcaac catgatgccg
      781 tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc gattttggag
      841 atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc gataacgtca
      901 gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca accaaagatc
      961 aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc aggcaggttc
     1021 acacctttgt tttcacccag gtcgccgttg ttcagcactt tacagataac tttgttacct
     1081 tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt gttgccaaca
     1141 gacaggtcag ttgtgaaacc ttcatacgtt accgcaacca tttcgctgtt gccgataaca
     1201 gatttatcag tagtgaaagt aaaggtctga ccagctttca gagaaacgtc gttaccgcct
     1261 tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc ggcggtttta
     1321 ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc tgcatagtca
     1381 ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt agctaacatc
     1441 tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt catgacagtc
     1501 ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact gccggaaagc
     1561 gaatccggta ttacgaaagt ggtggtgcct cgctctaagg ataggtgaca tcgaaaaagc
     1621 gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt tgagtcgttg
     1681 agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca ttatacgctg
     1741 aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact tgttagacaa
     1801 taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac ttcgctgttt
     1861 gcgcaacgtc tggtgccggg gttgtcactg tgaagataag tgaaagttca tccggtgtat
     1921 aaatacatca tcgatcaatt gctacagtta aaataattta tcgcgttatt tgtctgattt
     1981 tgtttgattt ttgttta
//
LOCUS       DAZGBC010000364         1953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGBC010000364 DAZGBC010000000
VERSION     DAZGBC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.366"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            202..456
                     /gene="bssS"
                     /locus_tag="ACHK1J_005212"
     CDS             202..456
                     /gene="bssS"
                     /locus_tag="ACHK1J_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO7914332.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            571..1689
                     /gene="solA"
                     /locus_tag="ACHK1J_005213"
     CDS             571..1689
                     /gene="solA"
                     /locus_tag="ACHK1J_005213"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO7914333.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1737..1850
                     /locus_tag="ACHK1J_005214"
     CDS             1737..1850
                     /locus_tag="ACHK1J_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO7914334.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          518 a          464 c          452 g          519 t
ORIGIN      
        1 aataattgca caatatgcta aaaaaatatg aatgacatgc tgttttattg ttcatataaa
       61 tcttaaaagc agaaatttat ctgtttcccc ctttaaaaat tcggtgaata cccttactta
      121 ttggtatact gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt
      181 aacacgaatt agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc
      241 gtagggtggg acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac
      301 cagaccccaa ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc
      361 actgatgttg ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc
      421 ggtgatttcc cggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa
      481 ggcacctcac cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc
      541 tgcttaaacc atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc
      601 ggttccgtag gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgcttatg
      661 accgacgccc atatgccacc acatcaacac ggcagccacc acggcgatac gcgattaatt
      721 cgccatgctt atggtgaagg cgaaaagtat gtcccactgg tcctccgcgc gcaaacactg
      781 tgggatgagc tctcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt
      841 aacctcggcc cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg
      901 caactcaacg ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc
      961 gtcccggaca attacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg
     1021 gcgattaaaa cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc
     1081 ccggtcaccg caattcgtca tgacgatgat ggcgtaacta ttgaaacggc tgacggtgag
     1141 tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag
     1201 ctgcctgtcc agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc
     1261 gtgaagaata aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt
     1321 tttccggcag aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat
     1381 tcagcggatg aacgtgttcc gtttgcggaa gtggccagtg atgggtcaga agccttcccg
     1441 ttcttgcgca atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat
     1501 gataattcgc ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg
     1561 ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct
     1621 gccgattttg cgcaagacaa aaaaagcgat tttgatttga cgccattcag gctttcccgc
     1681 ttccaataat catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc
     1741 gtcggctgtt gcactatctc atcaataata ttcgcgagca tctgatgctt tatctttttc
     1801 tctggggatt gttggcaatt atggatctga tctatgtgtt ttatttttag ttttccgcac
     1861 tgacataaac tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat
     1921 tattcctgct tctacagcac tcaccagaca tct
//
LOCUS       DAZGBC010000365         1937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGBC010000365 DAZGBC010000000
VERSION     DAZGBC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.367"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(711..>1937)
                     /locus_tag="ACHK1J_005215"
     CDS             complement(711..>1937)
                     /locus_tag="ACHK1J_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFO7914335.1"
                     /translation="RVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGVRVDIL
                     NVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQELDDYLS
                     REHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEFTIPEL
                     KMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGSYPLKL
                     EHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHDLLLEV
                     ILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDPECRGI
                     LILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLIAAVKQ
                     KYRTLIDYWREYRPAL"
BASE COUNT          500 a          489 c          506 g          442 t
ORIGIN      
        1 gtctgaagtg gcctactgaa atgaattgcg caggaaagtg agacggaaga tcggtcagac
       61 cgacaccagc aaaatatgat atatccgacg accatcaaag gtcataaagc cgataaggaa
      121 taggtgcgca gatatccgtc atggctctgc aaaaaactgt aaaatgaagc cgaatgtacg
      181 ggggcagtct gaaccccaac tgaagcatct tttttgcaca aaatcgggag tccatgtacg
      241 gatataaagc tgcaacctac cgtcaatgtc agaacaatgg aagctgtgaa aaacgggatg
      301 cgttaatata aactccatga cagtagtgga acgggttaat caagcaggat taccagcaag
      361 atgagggagc tgattcagca caggcgacct gaccctggcc acggataacc gtctgtcgcc
      421 cgtctgaggt gtcgtttttt cgttctgcca cccctcggtt gagtaaaatg gaaattaacc
      481 gtcaagctgc ctgtagcgta ccgatcagaa gattgctcag atggtacagc tgttgtacgt
      541 tctgcggcaa cgtgacgagg gaactaccaa tagccagaca gttcagaaat atcattaatg
      601 cagatacgaa acagcactgt cgggatgatg aggaaagtag gacggtcatg aaacatcttt
      661 gaatacgccc cgagcaagaa tacagacgcg ggacgtaatg cagacggata ctaaagcgcc
      721 ggacgatatt cgcgccagta gtcgatcaat gtgcggtatt tttgtttcac tgctgcgatg
      781 agctgttcat cgttaatctg gccgctgaac cactgccgtg atggctcgcc aaagatggtg
      841 cgacctacgg cgaagccttt aacccatggt atatccgcag cggcggcaaa gccagatttc
      901 agctcgcttt ccggtgcatc cagtccaagt attaaaatac cgcggcattc gggatcgtag
      961 cgctcaatat gctggctgat ttcttgccaa cgagccggac ttaacggtgg aagcttccac
     1021 cagtcaggct ggatccccag caggtaaaaa cgttccacaa tctgtgtata gtaatgctcg
     1081 ctttgatctg ctgcgtcgcg tggcaagatg acctccagca gcagatcgtg tccagactgg
     1141 cagcaggccc ggtatatctc atcaatgagt gcttcttgct taaggcggag ggattctgca
     1201 tccagtgggt gatagaagac aaggcatttc acgacgtgct ctttcggcca actgacaagt
     1261 tgtgaaccga tattaccgtg ctccagcttc agaggatatg accccggcat ttcgatagga
     1321 cgtccaatcc accatccctg accagtcacg tcgttaagtg ctttctgacc aaaagtagta
     1381 tccgccagga tgccgcagtt atgcagtaat ccagcctcgt gcgccgcctg acgtgcgcct
     1441 tccagtagta gcatcttcag ctcagggata gtgaactcgc tggcacctgt cgctctggcg
     1501 atatccacca gttgattgcg atggtcaaag gcgaatacac acagttccgg ccagtgctgt
     1561 tttcgggttg tcgttcggtg aagatggtta aggtgagggt ctttgtcggg tctggtgata
     1621 gcgtgctcac gggagagata gtcatccagt tcctgtttgg ttggcatcgc gggggcgcag
     1681 ccgtggcgcg atacaactaa tgcgccacat gcgttagcat aacgacaagc ttgctcccaa
     1741 ccttcatcgt tcagatagcc tcgaagcagt cccgacataa aggcgtctcc tgcacccagc
     1801 acatttagga tgtccacacg tacaccgctg tgcatctgca cctgagacca gtcgtcggcg
     1861 atgtttcctt caaatactga acaccctaat gcgccgcatt tgcataccaa taccgcagag
     1921 gttagctgac gcacacg
//
LOCUS       DAZGBC010000366         1927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGBC010000366 DAZGBC010000000
VERSION     DAZGBC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.368"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHK1J_005216"
     CDS             complement(<1..296)
                     /locus_tag="ACHK1J_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO7914336.1"
                     /translation="MSVVAPAVYVGTWHKYNGGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTG"
     gene            complement(761..1357)
                     /locus_tag="ACHK1J_005217"
     CDS             complement(761..1357)
                     /locus_tag="ACHK1J_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914337.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVSITDVLR"
     gene            complement(1354..>1927)
                     /locus_tag="ACHK1J_005218"
     CDS             complement(1354..>1927)
                     /locus_tag="ACHK1J_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7914338.1"
                     /translation="GSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVC
                     VLPLPSDAGHRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQ
                     IDLAFHTPATVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLI
                     FEAGEAGRGAPVQVRALEQWMIPNKLENAI"
BASE COUNT          454 a          598 c          495 g          380 t
ORIGIN      
        1 ccggtatcat ccacccagag acgataagcc tcttcgcagc cttcatcccg tgcctggcgg
       61 aagccttcaa cccaggccca gttgatatgg cattcagagg ccatattccc cgggaatccc
      121 tcataatcct gaaacatcag ttcaggatcg gcttcatcct ggtgaagagc acggcaggcg
      181 gcgaaaaaat cgcgttcatc atcaaacgtg gtcagatcaa accagcgtcc ggcgatgctt
      241 ccaccgttgt atttgtgcca ggttccgacg tatacagcag gtgcaacaac agacattccc
      301 gaatcctcca tagtcacatg ttctgcgaca ccgggcggtg ccgtgcctcc atctttgccg
      361 ggcctgcagg gatgcaaggg cgcaagggaa gccgcagccc gacgcgcacg gctgacggga
      421 gttggagggt gagaccgcga aatctgagcg ggtgaacgac cggaaaatca cggcagacgg
      481 gaaggaggga ccgggtgacc gcagcggttc atcttacctt gcatcccgcc cgcagaagcc
      541 cggctacgat ggaggcacgg cacagcccgc acacgacgct gaggccacag agctgaaaac
      601 ccacaaggcc atgccccgca tggccgggta aaagcggagc cgccggcgac ttccttattc
      661 cggtgagagc agaggacacg ccgcatgacc tccagaccac ccctgatatg aaacgatcat
      721 gaccggcatg tccctgcccc tcaagcatac ctccactggt tcaccgaaga acatctgtga
      781 tgctcacaag ttttctgtcg cgcagccagt cctgcgcttc acgcaccaca caactgtccg
      841 gtttcaccgc tttcagagaa gatgccagtc gcttgatcaa catcaccagt tcccgtgtct
      901 gtatgtccgg cattgtctcc ggtggagcag agggactctc atttccgacg cccatcccat
      961 gcgcttccag tgtggcacgg acaaaccagg cggcatcgac cagttgctgg cgaatccagg
     1021 ccagatcggc atttctgtca cgggctgcca ttcgctcacc cgacagcgcc ttgctgatat
     1081 ggctgcgcac cagattatcc aggagttcct gcgcttcttc cggtgaaaag ccacctggta
     1141 tctctgtgcg caacagtcct ttcctgtaca gtagctgcag cagatcaatc tgcgaccgca
     1201 ctgcaactat cagtacataa cggacaatat tcgcactggc ggtggtttct gtatcattca
     1261 gcagttcctc cggatcatta ccaccggtat tgcagagcaa cggcaacaga ggtcgcatcc
     1321 acacgggaac ctgatattgt tcgtctgatt tcatcatatt gcgttctcca gcttgttcgg
     1381 gatcatccac tgctcaagtg cacgtacctg taccggtgca ccacgaccgg cctcaccggc
     1441 ttcaaaaatc agccgccaga gtggttcctc ctgccagaaa aaccggtcaa atgatgacag
     1501 tgatggaaaa cgcccgcacc acccccagaa aatcgtttcc agatcatgct cctcaacaac
     1561 accagaccag cgggacaacc agcttccgac cgtggcggga gtatgaaacg caaggtcaat
     1621 ctgcgcctga gcaaaacatg tctggtattc cagttcccgt cgttctgcct cacgttcccg
     1681 ctggcggctg tatttctgct ctgtcagcgt ggttttctga cgacgacgct caccttcacg
     1741 aaaaggaacg gacgggaaca gacgatgccc cgcatcggat ggcaggggaa gaacacagac
     1801 ttcaccaaaa ctttcaatca gtttatccac ggcagccagc cacaggagac ggttgccatg
     1861 aaattcctcc ttcgtcagcg caggggcaaa aaagcggaga tcggtcagcg aaatccggcg
     1921 actgcca
//
LOCUS       DAZGBC010000367         1926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGBC010000367 DAZGBC010000000
VERSION     DAZGBC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.369"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..707
                     /gene="fabG"
                     /locus_tag="ACHK1J_005219"
     CDS             <1..707
                     /gene="fabG"
                     /locus_tag="ACHK1J_005219"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO7914339.1"
                     /translation="CIVTGAAQGIGRKIVEDFSKSEVKRVYACDIKHTDKSYPENVTP
                     VILDVCDRDSINTVISKIYSDEGRIDVLVNNAGVTRDNLINNMTEQDWDIVIDINLKG
                     VFNITQAVAPIMMEQNHGAIITMSSVVGTDGNIGQSNYAATKGGVIAMSKGWAKEFSR
                     KGAKVRVNCVAPGFVITPMTENLPEKVINHMIAKTPLGRMATPEDISNGVLFLASEKS
                     SFITGQTLKIDGGLVI"
     gene            717..1925
                     /locus_tag="ACHK1J_005220"
     CDS             717..1925
                     /locus_tag="ACHK1J_005220"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO7914340.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFALESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILTSEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVLKSISEN
                     HSVSIDWLNQRTNVNGGAIALGHPLGASGSRIVVSLLYQMIKNNKKRGLASLCIGGGM
                     GTAVIIELCS"
BASE COUNT          636 a          280 c          450 g          560 t
ORIGIN      
        1 tatgtatagt tactggagca gcacaaggta taggaagaaa aattgtggag gatttttcta
       61 aatcagaggt aaaaagagta tatgcatgtg atataaaaca tactgataaa tcctatccag
      121 agaatgtcac tccggtaatt cttgatgttt gcgatcggga ttcgattaat acagtaatta
      181 gtaagatata ttctgatgaa ggtagaatag atgttttagt taataacgca ggagttacaa
      241 gagataatct gatcaataat atgacagagc aagactggga tattgtaatt gatataaacc
      301 ttaaaggtgt ttttaatata actcaggcag ttgcacctat tatgatggaa caaaatcatg
      361 gggcaatcat aacaatgtct tctgttgtag gaactgatgg taatataggt cagagtaatt
      421 atgccgcaac taaaggtggt gttattgcaa tgtctaaagg ttgggctaaa gaattttccc
      481 gaaaaggtgc taaagtacgc gtgaattgtg tagctccagg attcgttatt accccgatga
      541 cagaaaatct gccagagaaa gtcattaacc atatgatagc taaaacccct ttagggcgaa
      601 tggcaacacc tgaggatatt tcgaatggtg ttcttttttt agctagtgag aaatcatctt
      661 ttattactgg tcaaacatta aaaattgatg gtggattagt tatttaagga ggaggaatgg
      721 aaaaagtata tattacaggg gcaaaaagaa cgcctatagg atgttttggc ggttcgttat
      781 caacagtgtc agcttcagat cttggagcaa ttgcgatatc tgcagcaatt aaacagagca
      841 gaataaacgt tgaatgtatt gatgaagtta ttgtcgggaa tgttttgtct gctggacaag
      901 gaatggggcc aggtagacaa gctgctgtta aagccggtat accatggaaa actccggctc
      961 tgactttgaa tatgatttgt ggtagtggta tgaaaactat tatcgatgct gcaatgaaaa
     1021 ttcgtagtgg agatatggat gttgttgtta cggcaggcat agagaatatg agtcaggcac
     1081 cttacttgat tgatcatcat gcaagaagtg gtgtacgaat gggtaatcag gtaatgagtg
     1141 actctcttat tacagatggt cttactgatg cttttaatca ctatcatatg gggattacag
     1201 cagaaaatat agcccgacag ttcaatatta caagatatga acaggataca tttgcgcttg
     1261 aaagtcagaa gagagcctta aaggccattg acaatggaga atttgaggat gaaattactc
     1321 cggtacaagt acatacccga aaaggaaata ttgaagttac tacagatgag tatcctcgta
     1381 gagactcctc attagagaaa ctttctgtgt tacgtccagc atttgaaaaa aatggtaccg
     1441 tgactgcagg aaattcatct ggaataaacg atggtgcagc agctcttatt cttacttcag
     1501 agaaagcaat tcagacattg gaattacaac ctttggctga aattcgctca tttggacaag
     1561 gaggcgttga tgcatcagtg atggggctgg ggcctgttcc agcaatagat aatgcattaa
     1621 aaaaagcaaa actggatatt aaagatattg atctttttga aattaacgag gcgtttgcgg
     1681 ctcaggcaat aggggtctta aaaagtattt ctgaaaatca ctcagttagt attgattggc
     1741 taaatcaaag aacaaatgtt aatggagggg cgatagctct aggccatcct ttgggagcat
     1801 ctggctcaag aattgttgtt tctttattat atcaaatgat aaaaaataat aaaaaaagag
     1861 gtctcgcctc cctttgtatt ggtggaggaa tggggacagc tgttattatc gaattgtgca
     1921 gttaac
//
LOCUS       DAZGBC010000368         1925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGBC010000368 DAZGBC010000000
VERSION     DAZGBC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.370"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..536
                     /locus_tag="ACHK1J_005221"
     CDS             <1..536
                     /locus_tag="ACHK1J_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO7914341.1"
                     /translation="GPSIIVANHNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKR
                     MAWFALKILNIIPISRKGGYTDPLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGV
                     WHLSQRMPEVPIIPVGLRGTEHVMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLE
                     QILTALLQPSQGNHPHD"
     gene            529..>1925
                     /locus_tag="ACHK1J_005222"
     CDS             529..>1925
                     /locus_tag="ACHK1J_005222"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO7914342.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGITDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPLITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPGADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSAVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFE"
BASE COUNT          408 a          478 c          564 g          475 t
ORIGIN      
        1 acgggccttc gataatcgtg gcaaatcaca acagccatat ggatgttttc gccttgcttt
       61 cgctttttcc gctgcgttac caggcttctg ttcatccggt tgctgccgcg gattattttc
      121 tgcgggataa acgaatggct tggtttgcgc tgaagatcct taacatcatt cctatttcaa
      181 gaaagggcgg ttataccgat ccgctggcgc aatgtgaaca ggcgctgcgt gaaggtaaaa
      241 cactgatcct gtttccggaa gggacgcgcg gtgagccagg cgtacttgcg ccattgaaat
      301 ccggagtgtg gcatttgagt cagcgcatgc cggaggtgcc cattatcccc gttgggttgc
      361 gcggtactga gcatgtgatg gcgaaaggta atcgtatccc tttgccgctg tttattgatg
      421 taaatattgg cgatgcgcta agtacgcatc cggagaagaa aacgtttatg gacgacctgg
      481 agcaaatact gacggcgctt ttgcaaccct cgcaaggaaa tcatccccat gactaattcc
      541 cgcatacccg atgaacggca gtttctgacc agtgataata cagagctgtt ttatcgccac
      601 tggccagcac aagaagccag ttcaacgcca aaagtggtgg tcctgtttca tcgtgggcat
      661 gagcactccg ggcgtttgca acacattgtt gatgagctgg cgatgccgga tacagcgttt
      721 tatgccatgg atgcccgagg tcatggtcgc tcaccgggtc tgcgtggtta cagtccgtcg
      781 ctcgtgcgtt cggtgcagga tgttgacgag tttgtgcgtt tcgccgccgc agatagccaa
      841 gctgggataa cggatgtggt cgtcgtggcg caaagcgtcg gggcggttct ggcggcaacc
      901 tgggcgcatg attatgcccc ggacattcgt ggcctggtgt tggcatcacc ggcgtttaag
      961 gtcaaattgt atgtgccgtt tgctcgccag gggctggaat tgatgtatcg cctgcgcggt
     1021 cttttcttcg tcaactcgta tgtgaaaggc aagtatctga cgcacgaccc cgagcgggta
     1081 gcgagcttca ataaggatcc gctgattacc cgaccaattg ctgtgaatat tctgcttgat
     1141 ctctacaaaa cggctgagcg agtcgtcagc gatgccagcg ccattacgct acctgtgcag
     1201 ttgctggtct ccggggaaga ttacgttgtg caccgccagc cgcagcttga tttttatcag
     1261 aacctgcgaa atccgcagaa agaacttcat gtactgccgg gattttatca tgacacctta
     1321 ggcgaaaaag agcggcatct ggcatttgac aggatgcgca gttttattga taccttgtat
     1381 gcccgtcccg tgcagcgttt tgattacagc caggaagatc gctggagccc cggcgccgat
     1441 acctggcgca tactgagcgg tggccctgcg ctgtattcgc tggacgatat ctcttaccgc
     1501 tgtttgcgct acgggatgaa agtgctgggc acgcagtcca cgggggtgcg tctgggattt
     1561 gagactggtt tcgattccgg cagtacccta gactacatct atcgcaacca gcctcagggc
     1621 agcacctcgt tggggcgcct cattgataaa aactacctga atagcgtggg ctggaagggg
     1681 atccgccagc gtaaaattca tctgcagatg ttaattcagc aggctgttgc tcggttaaca
     1741 gagcagggta gcgcggtccg gatcgtggat attgccgccg ggcatggacg ctatgtgctt
     1801 gatgccctgc aaggcgaaac ggcggtaagc gagatccttc tgcgcgatta cagcgaatta
     1861 aacgtggcga aaggtcagga gatgatagcc tctcgcggta tggcgggccg tgcgcgattt
     1921 gagca
//
LOCUS       DAZGBC010000369         1908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGBC010000369 DAZGBC010000000
VERSION     DAZGBC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.371"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1908
                     /locus_tag="ACHK1J_005223"
     CDS             <1..>1908
                     /locus_tag="ACHK1J_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HFO7914343.1"
                     /translation="VTSQTAGISVVTASINSSSQSRDVTFIADVRTAQIADLVVIKDG
                     SVADGAMANMLRARVTDAFGNALGGQTVSVTAGNSATVTPTVTTQSDGTVEFSVTSQT
                     AGVSAVTATINNHSLSQNVTFVPGDASQLTSTVETNKSNYTVGETITITVTLRDAFDN
                     LVTGAASQLAANSVLTVDGTDPSETGSWVESGGVYTTTRMATIAGTDQHANLQLQTWS
                     DGVTSDRYDIQSGSPAQATSTIATDKNAYTAGETITVAVTLKDAHGNLVEGGESLLSG
                     DNVTVEGAVRSGEWSETAGVYTATWSAQMAGDSHHATLTLPEWGNSKQSESYSIHSGA
                     PVQANSAIRTDKSAYIAGEPLTVTITLRDEFGNPALGLTSEVIESYIDSFAVGGATPD
                     SMQWVEQNNGEYTIVWTAWVAEENLVASLKLKTWATEIKSSLYGIQPGAAAQTQSTIV
                     TDKTKYIAGDSITVTVVLKDAQGNFITDGVAQLNEENVQVRNADSIQGNNWVYNGNGQ
                     YQRQYMAHFAEANLNAQLKMAGWSDANYSKNYTINRGEVSMLRSQLRIREVLVVAGAD
                     IPVSVLLSDEFGNPVNDGLDLLTDDAVYLQNVEKKHWSSWTFVGDGRYERTYMAYKEG
                     ENLNSYLHING"
BASE COUNT          521 a          426 c          534 g          427 t
ORIGIN      
        1 tgtcaccagc cagacggcag gaatcagtgt ggtcaccgcc agcattaaca gcagcagcca
       61 gagccgggat gtgacgttta tcgccgatgt cagaacggcg cagatcgcgg acctggtggt
      121 cattaaggac ggctcagtgg ctgacggcgc aatggcgaat atgctgcggg cgagggtcac
      181 cgatgcgttc ggcaatgcgc ttggcggaca gacggtcagc gtgacggcag gcaacagcgc
      241 aacggtcact ccgacggtca ccacgcagtc ggacggtacg gtggagttca gtgtcaccag
      301 tcagacggcg ggagttagcg cagtcacagc aaccatcaac aatcatagcc tgagccagaa
      361 cgtcaccttt gtaccgggtg acgcatcgca gctcacttca accgttgaaa caaataagtc
      421 taactacacg gttggagaga ccatcaccat cacggtaacg ctccgggatg cgttcgataa
      481 tctggtaacc ggtgcagctt cacagttagc cgctaatagt gtgctgacgg tggatggcac
      541 cgacccgtca gaaacgggaa gctgggttga atcaggtggc gtgtatacca caacccgaat
      601 ggcgacgatt gccggtaccg atcagcacgc taacctgcaa ctgcagacgt ggagtgatgg
      661 tgtaacgtca gatcgctatg acatacagtc cggttctccg gcgcaagcca catcaactat
      721 cgccacggat aaaaacgcat acaccgctgg cgagactata accgtcgccg tgacgctgaa
      781 agatgcgcac ggtaacctgg ttgaaggggg cgagtcctta ctgtctggtg ataacgtaac
      841 agtggaagga gccgtacgtt caggagagtg gtctgaaact gccggtgttt atactgctac
      901 atggtcggcg caaatggcgg gagactctca ccacgcgacg ctgacgttgc ccgagtgggg
      961 caacagtaaa caatcagaga gttattccat ccacagtggt gctccggtgc aggcaaattc
     1021 tgctatcagg acagataaat cggcatatat cgccggggag ccattaactg tcacaataac
     1081 gttgcgggat gagtttggta accctgcttt agggctgaca tcagaagtca tcgagtctta
     1141 tatcgatagt ttcgcggtag gtggcgcaac tcccgattct atgcagtggg tcgagcagaa
     1201 taatggtgaa tataccattg tctggacagc atgggttgcc gaggaaaatc tggttgccag
     1261 tctgaaatta aaaacatggg caacggagat taagtcctca ttatacggaa tacaaccagg
     1321 tgcggcggca caaactcaat ccacgattgt cacggacaaa acgaaatata tcgctgggga
     1381 tagcataacg gtgaccgttg ttcttaaaga tgcccagggt aactttatta ctgatggtgt
     1441 cgcgcagctt aatgaggaaa acgtacaagt caggaacgct gattctattc aaggcaataa
     1501 ttgggtttac aacggcaatg ggcaatatca aagacagtat atggcgcatt ttgcggaagc
     1561 gaacttgaat gcgcaactga agatggctgg ttggagcgat gccaattatt caaaaaacta
     1621 cactattaac agaggtgaag taagtatgct tcgttcacag cttcgtatac gcgaagtttt
     1681 agtggtagct ggggcggata tacctgttag tgttttatta tctgatgaat ttggtaatcc
     1741 tgtgaatgac ggtctggatt tgctgacaga tgatgcagtg tacttacaaa atgtggaaaa
     1801 gaaacattgg tccagttgga catttgtagg tgatggcaga tatgagcgta cctatatggc
     1861 ttacaaagaa ggagaaaact taaactctta tctacatatt aatggttg
//
LOCUS       DAZGBC010000370         1897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGBC010000370 DAZGBC010000000
VERSION     DAZGBC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.372"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..834
                     /locus_tag="ACHK1J_005224"
     CDS             1..834
                     /locus_tag="ACHK1J_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7914344.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(1050..1220)
                     /locus_tag="ACHK1J_005225"
                     /pseudo
     CDS             complement(1050..1220)
                     /locus_tag="ACHK1J_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          480 a          395 c          488 g          534 t
ORIGIN      
        1 atgatgaaac tgggattcaa tgaagcgacc tgtctgcgta attccagcct ggagaaggat
       61 ctggtcctag cggaacgttt tggatatgac tatattgaga tccgtctgga tatgttgcag
      121 acgtggctgc aggaacacac tctggttgaa ctggccgata ttttttctgt aagccatctt
      181 aagccatggg gctacaactc gctggaagac atcacatttt gtgatgccgg gcgctggtcg
      241 gaaatacggc aacaactggt tttagcctgt tatgcagggg aagtcgtagg tggtgattgc
      301 ctggttgtag tgccgaccat tcgtgaaggt gctcattttt cagatcgtga cacggtaaga
      361 gattcagtga aacgtttgcg tgagatgtca gcattagcgg cagagagcca gatgcgactg
      421 gcctttgagc ctatcggctc tgcgggatgt tgcgtacgca gtctggcgat ggcgatggag
      481 atcgtggacg cagttgatcg gagtaacgtt ggtctggcaa tagatgcctt taacctttat
      541 ctgtacgatc aatggcagga tctgagtacg ctgcgacagg ttccggtgga aaagatcttt
      601 gtctatcata ttgatgatgc tgaccgttta cctgtagctg tgttggatca ttgtcatcgt
      661 ctgtttccag gcaacggtgc gataccactg tacatgatca cccatgaact ggtgaaaaaa
      721 ggttatacag ggatctgttc actggagttg tttaacccag gttactggca aatggcagcc
      781 agcgaggtct ttgctatcgg agcggaaaaa acgcgacctt tcttagcgga ttaacgctga
      841 cgtaactgca aatgaactgt cgctgtgccc ggtaataagc aggtgatcag aataaatctc
      901 ctccggcata gccggaggtt tttcagatgt gtcttaaaga ttcgttttct gcctcgccct
      961 ggctgacagg cgcatatgat ctgacatttt cattcaactt ccttacttac gccccgtaaa
     1021 ttcacaagca tatggatatg atccgctcag cattcagctt ccagaatccg tacacttttc
     1081 cactcacaca gttttctcaa aatattgcct ggtgctcttg cacttcgctt ttctctgtag
     1141 aacatctgtc ttcggtattt tggcgcaaaa actatgtgat atttacagtt ccatcgggtg
     1201 ttcgttaagc cctttttcat tcacccattg gaatcccctt ttgatttctt gtttgacgct
     1261 tacagttggt agactgcaag gtgttttaac aaatcaaaaa ggggttttga caacagcctc
     1321 aaagttggaa gttttactga accactaggc tggctggtgg ttttctataa ataacaagaa
     1381 agatctgttg gtagtcaatg aatggcgtca gcaggcccta tgtcaatccg gtcagtcctc
     1441 aacttagagc ctgaaatgct gaataggggc caggggcggt tccgcggatt ttgggagagg
     1501 ccaagtgtac acctcatccc agtaacggaa aatttaactg attaataatt aattaacgaa
     1561 ttcagttctg tatatatcag gcctgatact cattttggtt acttaaatgg gtgaatgcag
     1621 aaaggttgct ctggtggtca acgtgatgac aaagacaggt aagacaggga ctcactaaac
     1681 ctaaatgggg ttttgggctt gaatcccgcg cagtaaatga ggtgtgagtc agcgaattac
     1741 ataggggctc gcagctttat ggttgcaata aagccggatc cccgggggaa tagatttaag
     1801 cccagcaggc acgtcgacca acgaatatcg tcatcataga gctggcaaat aagattgcca
     1861 ggatcgcatg ggcagtgttt atgcgtggtg aaaaact
//
LOCUS       DAZGBC010000371         1892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGBC010000371 DAZGBC010000000
VERSION     DAZGBC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.373"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(15..>1892)
                     /gene="mchF"
                     /locus_tag="ACHK1J_005226"
     CDS             complement(15..>1892)
                     /gene="mchF"
                     /locus_tag="ACHK1J_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO7914345.1"
                     /translation="LGMVTRALSLELDELGALKMPCILHWDFSHFVVLVSVKRNRYVL
                     HDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRISLRSLINSIYGIKRTLA
                     KIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISAGLMFFILLRAAVSMLRA
                     WSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSRFGSLDTLRATFTTCVVG
                     AIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTYGYYRQISEETLVRGARA
                     SSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKLTRMDLLFGGINTFVAAC
                     DQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLTSFLLQLRIMSLHNERIA
                     DIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQVFSGLNLSVAPGESVAIT
                     GASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIACVMQDDRLFSGSI
                     RENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIGELGEGLSGGQKQRIFIA
                     RALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVIIAHRETTLRTVDRIFRIT
                     N"
BASE COUNT          543 a          502 c          357 g          490 t
ORIGIN      
        1 agtgatatat tatcctaatt tgtaattcta aaaatccgat caactgttct taaagttgtc
       61 tctctatgtg caataatcac ccgggtgata ttcatctttt ttatagcagc attcacgaaa
      121 cgttcacttt ctgtatcaag agaacttgta gcctcatcca taaataatat tccaggtttc
      181 cggtataaag ctcgggcaat gaatatacgc tgtttttgac cgccggaaag accttccccc
      241 agttcaccta ttaacgtttc ataccccatt ggcattttca ttatcacatc atgaatatga
      301 cttgctttgg cacattctgt catccattcg tcgtctgttt cttctgcaaa cccacagata
      361 ttttcacgaa ttgatcctga aaatagccgg tcgtcctgca taacacaggc tatcatacgg
      421 tgataattat ttattccaag ttgacgtata tccgtgccat taaccagtac ttttccacta
      481 tctggttcaa acagtccaca taatactttc attaatgtgg tttttccggc accggaggca
      541 ccagttatag ccacactttc tcccggagcc acagacaaat tcagaccact gaatacctgt
      601 gctgactggc tgtcataccg gtagcttaaa tcagtggttt ccagtgaaac cgggctcatg
      661 tcagcaacga tttcaatttc cggtttcttt tcttcctttt catgtagtgc aatatctgca
      721 atgcgctcat tatgcagact cattattctc agttgaagaa gaaaactggt cagcgaagca
      781 acccgatccg aaaactgccc acgaaaagaa ccaaatgcca caaacatccc tattgtcatc
      841 tgattatcga tcacaaggct tgcacccagc cataaaatcg ccacctgatc acaggcggca
      901 acaaaagtat ttatcccccc gaagagcaaa tccatcctgg ttaacttaat acctgaattg
      961 atcgcatcta ttttcaggtt aagccagtgt gttccccgaa tcccagccat accctggatt
     1021 tttaccgtgg caataccata caggctttcc ataaaatagg agctggcccg ggcccccctg
     1081 acaagagttt cttccgatat ttgccggtaa tagccgtatg tcaccagacg aataagaacg
     1141 taaaccatgg taaaaccgag cactatccag gtaagatatc ctccatataa cagcatcatc
     1201 acaaaaacac ccacaaccat aatactgtcc atgattgccc caacaacaca ggtagtaaag
     1261 gtggccctca aagtgtcaag ggagccaaaa cgcgactgga tatcacctaa tttacggcgt
     1321 tcaaaaaacg ccagcggcag tctgagaaga tggttaaaca gacccgactg ccactgtata
     1381 ttgatgagcg tgctcataac cagtgaggac catgcacgca gcatactcac cgcggccctg
     1441 agcaatataa agaacatcag gccagcagaa ataagcgtca gcagccctct gtcccccgcc
     1501 ggaatcgcat gatccataac cagctgagtt cccaccggca ttaccagatt gattgcttca
     1561 attacaactg acagacagaa aattttcgcc agtgttcttt taataccgta aatactgtta
     1621 atcagtgaac ggagacttat gcgggtctgc tgggtttccg ccaggaattc acttccgggc
     1681 cagacctcaa gtgcaatgcc cgtaaaatac cggctcattt cctcccgacc gagatatctt
     1741 ctgcctctgg ccggatcatg cagtacataa cggttacgct ttacgctgac cagcacgacg
     1801 aagtgactga aatcccagtg gagaatacac ggcattttga gggcaccaag ttcatccagc
     1861 tccagtgaaa gagcccgggt gaccatcccc ag
//
LOCUS       DAZGBC010000372         1892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGBC010000372 DAZGBC010000000
VERSION     DAZGBC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.374"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            265..1209
                     /locus_tag="ACHK1J_005227"
     CDS             265..1209
                     /locus_tag="ACHK1J_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914346.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDEESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETNLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDILLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            1290..1712
                     /locus_tag="ACHK1J_005228"
     CDS             1290..1712
                     /locus_tag="ACHK1J_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914347.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
BASE COUNT          601 a          384 c          314 g          593 t
ORIGIN      
        1 ctattttaca taacatattt ttatcaaagt taattgattg ttgcctttat gagcaccgac
       61 cgtaagcccg ttatgttatt gtttcattag taataatcac ttaaatgtat cgatgaaaca
      121 ggtatgactt accgggagtg attgtttctg agtgaacaga ggccactttc gcctgataaa
      181 tgaaaaatga gcgatttttg caagttagtt tttctatagc cgagtacgct cagcgcctcg
      241 cttgattaca atgaggttgc tgctatgagt cctgataata tcggttcggc tgtcaatcgc
      301 ttctcaggtt ggccatctaa tgatcctcta aaaacagatg aagaatcaat gacatcaccc
      361 gtgactgtgc aagtaataaa gatgattgcc gccatccttg atccacatcg gatagtcgtt
      421 ggaattgata cactttatac aacgcagcgc gaaacattta cctcagagag tcaacagctc
      481 attgaatcag cattgcaaac acttcatgtg atgaatttaa ataacctgga aacaaatcgc
      541 gatgaagaag gtcactatct ggcttttgaa tataacgttg acacctctga cagaataacc
      601 gtttctgctt ataatgaaaa aaaacctggc tgctatggcc aatgtcttgt aatacaggat
      661 gacgaaacaa acttatttaa cctgataaaa attaaaattt tcgaagccag gcataattat
      721 gtcgctcaat tgatgagcga tccggaattt atgttcaaat ttagttatag cgctcaacaa
      781 aatagattag aacctcatct tctggttccc acttatttcc ctctaaaaac aaacgaaccg
      841 gttacgcaag aggatatact gttactttat cgtttcttta aaatggatgg tgattttaat
      901 atattaacat cagatgaata catgagtatc atgacacctc tgatgaagtg tgagcgcagc
      961 atccacgacc acaacaggta tgtgacgggc aaagataccc ttcttctgga ctatcctcct
     1021 tctggtaacc agatatattt ccatattttt cctgacgaat cagcaacact agtcctttat
     1081 ttttcaaaca atgacattga atgtttcgtc tttgaaagag atattccgag ccagtatcgc
     1141 tttttcaaaa tgtttaccaa tctggcactg gttattgatc agttaaccga tgccaatcaa
     1201 atactttaat ttttatatat cccctgtggc aaatattgct aaacaatgcc cggggattaa
     1261 tttttattac tcacggaaag atttaaataa tgataccatc tcagatctct ttcaatactc
     1321 ttcctgtcaa tgcaacatac gcttcagagc caacagttga cacacaaaaa ttctctgaca
     1381 tcctctactc agcggggtgt tccctaaagg atctggctta tctgagcaga tgtctggcat
     1441 caatacatcc gactatcgcc aaaaacattt atgaaacaga aaatctcagc gatcaaaaat
     1501 tactacatat tgattgccgt agtacaaatg aaatcaagat aaatatcttt tttggtcaac
     1561 aacgagaagg attaattgaa attaattctg acacagtcat tttttcgata ttgtcttcat
     1621 taattgactc cgccatttct aaatttcaac accaaatccc cgttaacggt tcagttaatc
     1681 gtgaactatt gtatgaggat tatactgatt aaatccaccc tgtcgcgaaa aatggcctgc
     1741 gttattgcca gacgtaataa aacctttgat taacgttact catactgata gcaccttcat
     1801 gtacctctct tttttacgaa aaatcaaaag agctaactaa ggtcataatt cgcatgaatc
     1861 attaaccatt tgttattttc aacattattt tt
//
LOCUS       DAZGBC010000373         1887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGBC010000373 DAZGBC010000000
VERSION     DAZGBC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.375"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..881)
                     /locus_tag="ACHK1J_005229"
     CDS             complement(<1..881)
                     /locus_tag="ACHK1J_005229"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7914348.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEFMTGDKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYVGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPSEVRQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAAL"
     gene            complement(871..>1887)
                     /locus_tag="ACHK1J_005230"
     CDS             complement(871..>1887)
                     /locus_tag="ACHK1J_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7914349.1"
                     /translation="DRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPAMLHIFEP
                     VEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEYIAG
                     APQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGDDLKLQTAQDSDNGFSALE
                     QALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLATQMFS
                     CWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIDSGL
                     STYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPEEETT
                     PRET"
BASE COUNT          428 a          607 c          468 g          384 t
ORIGIN      
        1 ttcagcgccg cagccatcac gctgatggca tccgacgcat tgattaattc atccgccagc
       61 ccggcctcaa taccggactg accttcaaaa acggcagcct ctgtccccgt gacagcttcc
      121 acagacagcc ccgtatacat cgccactttt tcggcaaaca tccggtgcgc cgcatcaatc
      181 cgctgctgca tgtcctggcg aacctctgac ggcaacgctt caaactgatt gccatccacc
      241 ttatgcgccc cggcataaat cagcgtgata tccactccgg cctgcgccag atgaccgaca
      301 tagctgacat ggcccatcat cacgccaatg gagccgatac gggatgtctg ggtaaccagc
      361 cgtcgggagc aggccgacgc cagcagcatg gccgcagaac aggccgtgtc attacacagt
      421 gcccagaccg gcttctgctg ccggaggcgg taaatcatgt cagcgcagtc aaacgcaccg
      481 gcggcctgcc cgcccggact gtcaatatcc agcagtacgc cccggacaga ggtgtccgcc
      541 attgcctgct gaagacaggc aacaatgccg tcatagcctg tcattccgga aaatggccgc
      601 atgccaccca gccggtgcac cagcgtgccg gtcaccggca gtacagcaat accgttcacc
      661 acccggtaaa cacgggccgg tcgtttatct ccggtcatga actcatccgt ttcagccagc
      721 attccgggag catcaagctg tacctgctgt tgtggtaccg aaagacttgc tgcccccatc
      781 tcgcgcccca gcgcgcaaaa gaaaacccgc gcataggcgg gctccagaag cagcggttca
      841 ttgaatgctg cggcaataat gtgtgaaaga ttacgtctca cgtggtgttg tctcctcttc
      901 cggcctgcgg ctctccgcta tctgctgctg atacgcctgc gctatccaca ccggacgtga
      961 gagtccggct ttttcccgct ctgcggattc cctgacctgc tggcggaaaa tgtcctgata
     1021 atcctcaccc atcagcgcca gctctttctc atacgtgctc agtccggaat caatacgcat
     1081 caccgattcc tggacctcct tgagcccgtc aatggccatt cttccggcac caatccactc
     1141 ggcccgtgac caggctgatc gcgcctgata aaaatcaaaa cgtgcccgtg gcggacggat
     1201 aatcccccga agaagtgcct cttccagcca gcaggaaaac atctgcgtgg ccagccgggc
     1261 cgcaataaat tttcgtcgtc ccataaaata gcgccacgac tcattggcag aggcgcgggc
     1321 acttgaataa ctgaccttcg agtaatcacg ggacaactgt tcgtaggaaa cgccaagacc
     1381 ggcggcgata taccgcagca gcgcctgttc aagcgccgaa aatccattgt ctgaatcctg
     1441 cgcggtctgc agtttcagat catcaccggg gaaaaggtgc ggaattttga caccgcccag
     1501 cgtcacatga ttcgtgtcat accagctgga gaacttctcc agaatattaa taagcggatt
     1561 atcctgctga ccctgcggtg cccccgcgat atattcaaag gccttttcgg tatcaagttc
     1621 actttcaatc gtcgctgcat acatggcttt aacaatggcc gactgaagct gtgttgcctg
     1681 cagggaatcg agcatcttca gccgttccat gacgctgtaa aactgattgg ccccacgggt
     1741 ctgcccgtcc tccaccggct cgaaaatatg cagcatggcc ggacgcccgg tgggaagttc
     1801 acgcgggatc cgttcccatc gtccactacc agagaacgga aaatcatcct cacagatatg
     1861 gtacgcaacg gcacggccat atcgatc
//
LOCUS       DAZGBC010000374         1887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGBC010000374 DAZGBC010000000
VERSION     DAZGBC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.376"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..881)
                     /locus_tag="ACHK1J_005231"
     CDS             complement(<1..881)
                     /locus_tag="ACHK1J_005231"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7914350.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSLPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPSEVRQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAAL"
     gene            complement(871..>1887)
                     /locus_tag="ACHK1J_005232"
     CDS             complement(871..>1887)
                     /locus_tag="ACHK1J_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7914351.1"
                     /translation="DRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPSMLHIFEP
                     VEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEYIAG
                     APQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGDGLKLQTAQDSDNGFSALE
                     QALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLATQMFS
                     CWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIDSGL
                     STYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPEEETT
                     PRET"
BASE COUNT          429 a          606 c          468 g          384 t
ORIGIN      
        1 ttcagcgccg cagccatcac gctgatggca tccgacgcat tgattaattc atccgccagc
       61 ccggcctcaa taccggactg accttcaaaa acggcagcct ctgtccccgt gacagcttcc
      121 acagacagcc ccgtatacat cgccactttt tcggcaaaca tccggtgcgc tgcatcaatc
      181 cgctgctgca tgtcctggcg aacctctgac ggcaacgctt caaactgatt gccatccacc
      241 ttatgcgccc cggcataaat cagcgtgata tccactccgg cctgcgccag atgaccggca
      301 tagctgacat ggctcatcat cacgccaatg gagccgatac gggatgtctg ggtaaccagc
      361 cgtcgggagc aggccgacgc cagcagcatg gccgcagaac aggccgtgtc attacacagt
      421 gcccagaccg gcttctgctg ccggaggcgg taaatcatgt cagcgcagtc aaacgcaccg
      481 gcggcctgcc cgcccggact gtcaatgtcc agcagtacgc cccggacaga ggtgtctgcc
      541 attgcctgct gaagacaggc aacaatgccg tcatagcctg tcattccgga aaatggccgc
      601 ataccaccca gccggtgcac cagcgtgccg gtcaccggca gtaccgcaat accgttcacc
      661 acccggtaaa cacgggccgg tcgtttacct ccggccatgt actcgtccgt ttcagccagc
      721 atcccgggag catcaagctg tacctgctgt tgtggtaacg aaagacttgc tgcccccatc
      781 tcgcgcccga gcgcgcaaaa gaaaacccgc gcataggcag gctccagaag cagcggttca
      841 ttgaatgctg cggcaataat gtgtgaaaga ttacgtctca cgtggtgttg tctcctcttc
      901 cggcctgcgg ctctccgcta tctgctgctg atacgcctgc gctatccaca ccggacgtga
      961 gagtccggct ttttcccgct ctgcggattc cctgacctgc tggcggaaaa tgtcctgata
     1021 atcctcgccc atcagcgcca gctctttctc atacgtgctc agtccggaat caatacgcat
     1081 caccgattcc tggacctcct tgagcccgtc aatggccatt cttcctgcgc caatccactc
     1141 tgcccgtgac caggctgaac gcgcctgata aaaatcaaaa cgcgcccgtg gaggacgaat
     1201 aatcccccgc agaagtgcct cttccagcca gcaggaaaac atctgcgtgg ccagccgggc
     1261 cgcaataaat tttcgtcgtc ccataaaata gcgccacgac tcattggcgg aggcgcgggc
     1321 acttgaataa ctgaccttcg agtaatcacg ggacaactgt tcgtaggaaa cgccaagacc
     1381 ggcggcgata taccgcagca gcgcctgttc aagcgccgaa aatccattgt ctgaatcctg
     1441 cgcggtctga agtttcagac catcaccggg gaaaaggtgc ggaattttga caccgcccag
     1501 cgtcacatga ttcgtgtcat accagctgga gaacttctcc agaatattaa taagcggatt
     1561 atcctgctga ccctgcggtg cccccgcgat atattcaaag gccttttcgg tatcaagttc
     1621 actttcaatc gtcgctgcat acatggcttt aacaatggcc gactgaagct gtgttgcctg
     1681 cagggaatca agcatcttca gccgttccat gacactgtaa aactgattgg ccccacgggt
     1741 ctgcccgtcc tccaccggct cgaaaatatg cagcatggac ggacgcccgg tgggaagttc
     1801 acgcgggatc cgttcccatc gtccactacc agagaacgga aaatcatcct cacagatatg
     1861 gtacgcaacg gcacggccat atcgatc
//
LOCUS       DAZGBC010000375         1880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGBC010000375 DAZGBC010000000
VERSION     DAZGBC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.377"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            420..629
                     /locus_tag="ACHK1J_005233"
     CDS             420..629
                     /locus_tag="ACHK1J_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7914352.1"
                     /translation="MSEDVPLPKANQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(649..882)
                     /locus_tag="ACHK1J_005234"
     CDS             complement(649..882)
                     /locus_tag="ACHK1J_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914353.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(977..1600)
                     /locus_tag="ACHK1J_005235"
     CDS             complement(977..1600)
                     /locus_tag="ACHK1J_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7914354.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKHFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNLTGTTRKIPAYHL"
     gene            complement(1689..>1880)
                     /locus_tag="ACHK1J_005236"
     CDS             complement(1689..>1880)
                     /locus_tag="ACHK1J_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914355.1"
                     /translation="TVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYV
                     ALKTAVQSLMPRGIATIIR"
BASE COUNT          533 a          490 c          407 g          450 t
ORIGIN      
        1 ccattatagg acgaagatct gcaaattctc aatatgacag acatatcgat cgttttaatg
       61 ccaataacca gatctttatt ctgatgcata tcgcaaaatc agcatttatc aaaacttata
      121 tccaggagta atacaaaaat gcagaaaatt attccggtta gtacgtttga tacagatggt
      181 tataccgaat gattaccagc actgacattc acgaggagtg atgattcggc agcatttaag
      241 ggcatatcag aaaaagagaa ggacatgaaa gaggagagca ataaaagaca cggaaaataa
      301 agaagagaga gaaaatattt cttaaaaaca gaagattaac aaaacacatc aggaggcaga
      361 gactcctgat tcagcaatat cacacgaaat tttagttaac tgctaccgtt ctgaaatcta
      421 tgtctgaaga tgttccatta ccgaaagcca atcagcgata cagagacgat catggtgcgc
      481 ttgtcaccgt gaccagtgtt gaggaaaccc gggtggtatt tatgcgggac ggctatccgc
      541 atccctgcat gcggccgatg tacaacttcc tgggaaaatt caaacccgaa ccccggaaag
      601 aaccccctgc cggcaatcac actgcctgat ggcagcataa tccacttatt aattctcatc
      661 cccggagaca agccctgaaa accactcggg atgttcactg tatgctgcgt tcagagcatc
      721 ccaggaaacg ttcatacaga agctgtcctg caatttgcag gcaaccacct ctgccaggcg
      781 gttccggtta tccgccagtc cggcataacc tctctctgcc agccactcat tcagttcatg
      841 ttccagagac agattgataa aatcgtaatc ggaccttgcc attcccgttt ccttaacaca
      901 tgtcttcaga ctcagggata aagtataaaa atgtcgccct gctgctgcca gagcaatatt
      961 ccccctgatg gccgtatcag agatggtatg ccggtatttt acgggtggtt cccgtcaggt
     1021 tatcgtgtac ctccacgctc gtagtcacca ccggcattcc ggcctttctc agcctcaaaa
     1081 catcagctgc aatacgctga ctaccagacc agaacaggcc gtccagtgca gtcaccagta
     1141 accccgcctc cagcgcatac ttcagccgtt cacggggcgc tttcacttcc cgggcaacct
     1201 gctgatatgg caatgatgtg ttttcactcc cgaccatccg gcgaatacga tgagacagat
     1261 gataccggta tgtatccggc acacctgaaa ggctggcctt caggctgtac acgcagccaa
     1321 agtgtttatc gttgaacacc acatttttct ggctgatatc ccattcttca cgcagcgcgg
     1381 caatcagttg tggtgtacgg gtaagcaaca agcgaaaagg cagttcaaaa ctggtgacgt
     1441 aatccacatt cagcagggca atgcgaagtc gttcttctgg tccggcttct gtctgccggc
     1501 actcctccag gacatcctgc cactgcaggc gaagacggga agactcattc agttctgtaa
     1561 agcagtattt atccgccaga tagtcaattc gtgtatgcat actgaagagt attccgtata
     1621 aagattcagc tggcaaaact ttatcagtct gtaaaaacta acggaagagt cgatatttct
     1681 cccgtccatc accggatgat tgttgcaata ccccgtggca tcagagactg aacagccgtt
     1741 tttaacgcaa cgtattgctc tgatgtatca ggccggacaa cccgaaaaca cctttccacc
     1801 cggcattgtc cgccagcgct tatcaccggc caggtctgtt gcagtaaatc cgccacttgc
     1861 gaacatgctt catcaactgt
//
LOCUS       DAZGBC010000376         1877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGBC010000376 DAZGBC010000000
VERSION     DAZGBC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.378"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..536
                     /locus_tag="ACHK1J_005237"
     CDS             <1..536
                     /locus_tag="ACHK1J_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO7914356.1"
                     /translation="GPSIIVANHNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKR
                     MAWFALKILNIIPISRKGGYTDPLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGV
                     WHLSQRMPEVPIIPVGLRGTERVMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLE
                     QRLTALLQPSQGNHPHD"
     gene            529..1877
                     /locus_tag="ACHK1J_005238"
                     /pseudo
     CDS             529..1877
                     /locus_tag="ACHK1J_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126693.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT          401 a          465 c          548 g          463 t
ORIGIN      
        1 acgggccttc gataatcgtg gcaaatcaca acagccatat ggatgttttc gccttgcttt
       61 cgctttttcc gctgcgttac caggcttctg ttcatccggt tgctgccgcg gattattttc
      121 tgcgggataa acgcatggct tggtttgcgc tgaagatcct taacatcatt cctatttcaa
      181 gaaagggcgg ttataccgat ccgctggcgc aatgtgaaca ggcgctgcgt gaaggtaaaa
      241 cactgatcct gtttccggaa gggacgcgcg gtgagccagg cgtacttgcg ccattgaaat
      301 ccggagtgtg gcatttgagt cagcgcatgc cggaggtgcc cattatcccc gttgggttgc
      361 gcggtactga gcgtgtgatg gcgaaaggta atcgtatccc tttgccgctg tttattgatg
      421 taaatattgg cgatgcgcta agtacgcatc cggagaagaa aacgtttatg gacgacctgg
      481 agcaaagact aacggcgctt ttgcaaccct cgcaaggaaa tcatccccat gactaattcc
      541 cgcatacccg atgaacggca gtttctgacc agtgataata cagagctgtt ttatcgccac
      601 tggccagcac aagaagccag ttcaacgcca aaagtggtgg tcctgtttca tcgtgggcat
      661 gagcactccg ggcgtttgca acacattgtt gatgagctgg cgatgccgga tacagcgttg
      721 ttacagtccg tcgctcgtgc gttcggtgca ggatgttgac gagtttgtgc gtttcgccgc
      781 cgcagatagc caagctggga tgacggatgt ggtcgtcgtg gcgcaaagcg tcggggcggt
      841 tctggcggca acctgggcgc atgattatgc cccggacatt cgtggcctgg tgttggcatc
      901 accggcgttt aaggtcaaat tgtatgtgcc gtttgctcgc caggggctgg aattgatgta
      961 tcgcctgcgc ggtcttttct tcgtcaactc gtatgtgaaa ggcaagtatc tgacgcacga
     1021 tcccgagcgg gtggcaagct tcaataagga tccgctgatt acccgaccaa ttgctgtgaa
     1081 tattctgctt gatctctaca aaacggctga gcgagtcgtc agcgatgcca gcgccattac
     1141 gctacctgtg cagttgctgg tctccgggga agattacgtt gtgcaccgcc agccgcagct
     1201 tgatttttat cagaacctgc gaaatccgca gaaagaactg catgtactgc cgggatttta
     1261 tcatgacacc ttaggcgaaa aagagcggca tctggcattt gacaggatgc gcagttttat
     1321 tgataccttg tatgcccgtc ccgtgcagcg ttttgattac agccaggaag atcgctggag
     1381 ccccagcgcc gatacctggc gcatactgag cggtggccct gcgctgtatt cgctggacga
     1441 tatctcttac cgctgtttgc gctacgggat gaaagtgctg ggcacgcagt ccacgggggt
     1501 gcgtctggga tttgagactg gtttcgattc cggcagtacc ctagactaca tctatcgcaa
     1561 ccagcctcag ggcagcacct cgttgggacg cctcattgat aaaaactacc tgaatagcgt
     1621 gggctggaag gggatccgcc agcgtaaaat tcatctgcag atgttaattc agcaggctgt
     1681 tgctcggtta acagagcagg gtagctcggt ccggatcgtg gatattgccg ccgggcatgg
     1741 acgctatgtg cttgatgccc tgcaaggcga aacggcggta agcgagatcc ttctgcgcga
     1801 ttacagcgaa ttaaacgtgg cgaaaggtca ggagatgata gcctctcgcg gtatggcggg
     1861 ccgtgcgcga tttgagc
//
LOCUS       DAZGBC010000377         1872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGBC010000377 DAZGBC010000000
VERSION     DAZGBC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.379"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..288
                     /locus_tag="ACHK1J_005239"
     CDS             <1..288
                     /locus_tag="ACHK1J_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HFO7914357.1"
                     /translation="QDFTSSTQDRVGTVKVPLLEPADIVTLPKGQAFALLEGGQLWKI
                     RMPLPATDADDALMPESIAKIAEAMRRNYHTGEGWWNETPSVNMPEGEQNG"
     gene            281..1042
                     /locus_tag="ACHK1J_005240"
     CDS             281..1042
                     /locus_tag="ACHK1J_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO7914358.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSAPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLYEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            1675..>1872
                     /locus_tag="ACHK1J_005241"
     CDS             1675..>1872
                     /locus_tag="ACHK1J_005241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914359.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQG"
BASE COUNT          423 a          447 c          543 g          459 t
ORIGIN      
        1 caggacttca catccagtac ccaggatcgt gttggtactg tgaaggtgcc tttgctggag
       61 ccggcagata tcgtgaccct gccgaaagga caggcctttg ctctgctgga agggggacag
      121 ttgtggaaaa tccgtatgcc cctgcctgca actgatgcag atgacgcgct gatgccggag
      181 agtattgcaa aaattgcaga ggccatgcgg cgtaattacc acaccggaga gggatggtgg
      241 aatgagactc cctccgtgaa catgccggaa ggagagcaga atggctgaac cccgtcgtga
      301 ggtgaacaga gcagcacaac cctctccgcc acgtcagcac ggactgctct ggaatctgtt
      361 gtggggctgg ccgtggatgc tggtcgggat cctgttttcc tccctgctgc tgagcctgct
      421 ggttgagtat atcgggattg cttttttctg gccggagcag ggggcagcac acagtgaggc
      481 agtgatgaag acagaactgg ggtggctgtc ttcggaattc acccgcagtc tgctgctgtc
      541 ggcaccatcg gtaacggtga cagaatggat agcaacggcg tatcagtggc tgtttgtgga
      601 aacaggtttt ctgtcgtggc tgaagcagca aaatgccggt gccgtggtgg gtaacagtgt
      661 gtcacagtac agtgaggatg tggggcgctg gctgagccat tatctgtatg agtacctgat
      721 ggccacagta tatgtcacgg tggtcacact gatacgggtg acgatcctgg tgttgtctgt
      781 tccgctgttt gtgatggtga tactgatcgc ggttgtggag ggactggggc gtcgtgatct
      841 gcgccggtat ggtgctgcgt atgagtccag ctttgtttat caccatgcaa aaaagtctat
      901 taaaccggcg atatatattc cctgcattct ttacctgtcc tggccgacgt cggtgtatcc
      961 gaacctgctg atatggccgg cagccatgct gctgggggtg gccatcaccg tgaccatggc
     1021 atccttcaag aaatatttat aatccacaga ggccggacac cctgatgcaa aacttgtttt
     1081 gtggcagggt gtcggcagac aagccacgcc cttacggtca gtgcgcgtca gtgtcagagt
     1141 aacgacagcg ccggaatgac aacaatgttg acgaaaataa tgaacagagt gatggccctg
     1201 atgacgtcat agatccacgg ggggcaacga ggttcttcat aaatgaaaaa atacacaaca
     1261 caaacccagg tcagaaggaa cagggcccag aacggaaggg cagtacggag aaattccatg
     1321 ctgatgtgtt tcctctttaa attgttaatg cggtgccatt aacgggaaac agtagtgatg
     1381 ccgtgtttta ataaacagga ataaatcatt caggctggaa taaatttatt tgaacagaat
     1441 ctggaacagc gagatgcaga aactggctat gctgacagcc cccagaacat agtttattgt
     1501 tgcccgtatt gcccgtattg cccgtattgc ccgtattgcc cgggttgttt gccttcatcc
     1561 acaccagtgt attctcccgt aaaaattttc tgttttgcaa atagagttta tcgctgatcc
     1621 agtcccctgc cttacgcatc ctgtacgcac tgtttaactc tgacggaggt gcgtatgttt
     1681 tcttttccgg catcatcctg ccgtgccggc cgtctggccg gtgccgcggg gatgctgttt
     1741 cttttgtcct ttgcattacc tgcgcaggct gccgaaaagg atgaactggc cactgtaatg
     1801 cgccagcttg accacgtgca ggcagcgctg gagcgggcca gaacggcctc tctgcaggct
     1861 tcttcacagg gg
//
LOCUS       DAZGBC010000378         1866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGBC010000378 DAZGBC010000000
VERSION     DAZGBC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.380"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1110
                     /locus_tag="ACHK1J_005242"
     CDS             <1..1110
                     /locus_tag="ACHK1J_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7914360.1"
                     /translation="GDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELARTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVKQMQNQ
                     MDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWYAEYDEWGNQLNEENPHQLQQLIR
                     LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLA
                     SKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSP
                     YDYTLYCRKWVKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYL
                     FPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1107..1676
                     /gene="ybfC"
                     /locus_tag="ACHK1J_005243"
     CDS             1107..1676
                     /gene="ybfC"
                     /locus_tag="ACHK1J_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO7914361.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWKWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKIQHELLRSEEK"
BASE COUNT          580 a          403 c          449 g          434 t
ORIGIN      
        1 ggcgaccgcc tgaccacgat acagaacgac agaacccgca tccagacgat ttatcagccg
       61 gggagcttca caccactcat cagagttgaa actgccaccg gtgaactggc cagaacgcag
      121 cgccgcagcc tggcggatgc ccttcagcag tccggcggtg aagacggtgg cagtgtggtg
      181 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      241 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgaa gcagatgcaa
      301 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      361 cgcggcctgc cgctggcgct tatcagcacg gaagggacaa cagcgtggta cgcagaatac
      421 gatgaatggg gtaaccagct gaatgaagag aacccgcacc agctgcagca gctcatccgc
      481 ctgccgggtc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      541 gacccgctgc aggggaggta tatcactcag gatccgattg gactgaaggg gggatggaat
      601 ttttatcagt atccgttgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      661 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      721 ccaagtaaat tgccagacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      781 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      841 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgatccccag
      901 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
      961 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1021 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taaaatgaaa
     1081 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1141 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1201 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1261 ggaaatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1321 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1381 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1441 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1501 agagcagtga tccagctcag ttgttggcag tccttgataa ttcaatcatg gttaaatatt
     1561 ttaaagatga gaagccaact tatgctcgaa tgacagcaca tctcccgaac aaagatgagt
     1621 cttacgaatg tttaacaaaa atacagcatg aactgcttcg tagtgaagaa aaataaaaca
     1681 tcttcctgag agaataaatt aaccattcgg aaaaacatgt tcttaatgga tatccaggta
     1741 caccagaatg tcgatttgga aaaatggatt ccgatggtaa gaatatcata tattctgaat
     1801 gtaatgtttt acccacagct tttgagattc gataggtgta gaacatgaga aactcactaa
     1861 tattac
//
LOCUS       DAZGBC010000379         1861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGBC010000379 DAZGBC010000000
VERSION     DAZGBC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.381"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..1308
                     /locus_tag="ACHK1J_005244"
                     /pseudo
     CDS             1..1308
                     /locus_tag="ACHK1J_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1341..1523)
                     /locus_tag="ACHK1J_005245"
                     /pseudo
     CDS             complement(1341..1523)
                     /locus_tag="ACHK1J_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1520..>1861)
                     /locus_tag="ACHK1J_005246"
     CDS             complement(1520..>1861)
                     /locus_tag="ACHK1J_005246"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFO7914362.1"
                     /translation="ELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLAAAIEPGGIL
                     IYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKCTQLIDEMGI
                     FTVSMAVRRQP"
BASE COUNT          450 a          491 c          531 g          389 t
ORIGIN      
        1 agaacatctg cgggctcaga tagaaaaact gcgccagttg cgacagtcgt gccatcgtcg
       61 tccgttaccg gcacaccttc cccgtgaaat acaccgtctg gagtctgaag aaagctgttg
      121 cccggagtgt ggcggtgagc tggattatct gggggaggtc agtgcagaac aactggaact
      181 ggtgagcagc gccctgaaag tgatccgcac agaacgggta aaaaaagcct gtacaaaatg
      241 tgactgcatc gttgaagcac cggcaccgtc ccgcccgata gagcgtggta ttgcgggccc
      301 cggattactt gcccgcgtgt taacgggaaa atactgcgaa cacctgctac tgtatcgtca
      361 gagtgaaatc tttgcctgac agggtgtcga actgagccgt gcattactct ccaactgggt
      421 tgacgcgtgc tgccagttaa tgacgccgct gaatgatgcc ctgtaccgtt atgtgatgaa
      481 tacccgcaag cttcacactg acgacacacc ggtaaaggta ctggcaccgg gccagaaaaa
      541 gacgaaaaca gggcgcatct ggacgtatgt ccgggatgat cgcaatgcgg gttcgtcatc
      601 tcctccggcg gtctggttcg cgtactcacc gaaccggcag gggaaacacc cggagcagca
      661 cctccgcccc ttccggggta tcctgcaggc ggatgcgttc acaggttacg acaggctgtt
      721 cagtgcagaa cgtgaaggtg gtgcgctgac agaagttgcg tgctgggccc atgcccggag
      781 aaaaattcac gatgtataca tcagcagcaa aagtgcgacg gcagaagaag ccctgaagcg
      841 aatcagtgaa ctgtacgcca tcgaggatga aatacgggga ttaccagagt cagagcgtct
      901 tgctggcagc agcgaagcaa atcgttactg acgtcgctgc atgaatggat ggtggagaag
      961 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1021 tgggacgccc tctgttatta cagtgatgac ggtctggcag aggcggataa taatgctgcg
     1081 gaaagagcgc ttcgtgcagt ctgtctcgga aagaaaaact ttatgttctt tggcagcgat
     1141 cacggcggcg agcgtggagc actgttgtac gggctgatcg gcacctgccg tctgaacggt
     1201 atcgatccgg aagcgtatct gcgccatatc ctgagcgtac tgccggaatg gccttccaac
     1261 cgagttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     1321 tgctccgttg acgcttacct ccagtacgga ataatcgtca gcggtattaa cgggatccgg
     1381 tgctcccagt caaacaccag gctgctgatg ttttctctcc cggcggtaaa ctgattcgtc
     1441 atgccataag taagaaagaa gaacggggcc agcaccatta gccagccgat tccctgaata
     1501 atcaccgcac gccgcgttgt catggttgac gtcgtaccgc catagagacc gtgaagatgc
     1561 ccatttcatc aattagttgc gtgcatttct caaaaccggc gtcacggacc agcgcgtcca
     1621 tctcgccctg agtacgtacg cgcattaccc aggctttgcc atttttgtga ctggtgagtg
     1681 accaggcgat ggtttttaac tgagggtgcc agggctgaca ggtgtagatc agaatgcctc
     1741 ccggttcaat cgctgcagcc agcccggcaa gcgaatctcg taccggcccg ttttccggga
     1801 acagttcata cagaccggac acgatcccca gcgtcggacg cggttccagt gccgccagtt
     1861 c
//
LOCUS       DAZGBC010000380         1853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGBC010000380 DAZGBC010000000
VERSION     DAZGBC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.382"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            394..1512
                     /locus_tag="ACHK1J_005247"
     CDS             394..1512
                     /locus_tag="ACHK1J_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7914363.1"
                     /translation="MTKLYIYLVLAASLSALSTDMIVPALPELQKAFHADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANDLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKETNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFHYSVVFSLSVLFFIIGNQLSKIEKISNPWVLFPLNAISVTPFLIL
                     NDAFIICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISFL
                     SVKISDNGVFLGTTLSISCLILALLSMFVTIIIGNNKK"
     gene            1509..>1853
                     /locus_tag="ACHK1J_005248"
     CDS             1509..>1853
                     /locus_tag="ACHK1J_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFO7914364.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKHAIANAIEKFGVQLSVARTRVKVDLFEQLEKRLNKIMNGA
                     HTLTFNAVTPCHL"
BASE COUNT          624 a          334 c          316 g          579 t
ORIGIN      
        1 atttaccata atcccttaat tgtacgtacc gctgaaacgc tctcatacgc gttctgacgc
       61 aaaaacgcaa aaagacaagg aaaccccagg ctaaaccgaa aaaatgcgtc agaatcgttt
      121 tcaggtactt gcataactat tcacaaaagc gatgcgcgat cgatttcgac tattccgacg
      181 gacgaacagg ttaaaatgat aagtttcctc cctccggatg gaaggttcag cacggggatt
      241 tttaccccct tccatcaacc tgaatatgca tctgggagta cccattagtt tctactcaaa
      301 cactatcgta gtgctatctc aaaatataaa acataccgat ggcgtaagca ttttaaatat
      361 gataaataag aatattttta tcagggatta attatgacaa aactatatat ttaccttgtt
      421 cttgctgcat cattatcggc attatcaact gacatgattg tgcctgcgtt accagaacta
      481 cagaaagcat ttcatgctga ctactcatta attcaactaa gtattagtgg atttcttatt
      541 gtatttgcat ttagtcaact gatatcagga accataggtg attatttcgg aaaagtacgc
      601 gtaatgacta tatgcttaat tatatttctt attggcagta ttgtatgttt tatggccaat
      661 gatttaaata tgcttctttt gggtagaatt ctacaggcaa taggtgcagg tgccggacct
      721 gtaatatcaa aagccattgc taaagagtct tttccaccac taaagctaaa aagagcactt
      781 tctgacatat catcaacaag tgcgataata ccattaatag cacctctggc cggagcatta
      841 atacttgatt attataactg gaatgtaata tttattgtaa tgggtgcatt tagcattcta
      901 accatactcc tgtcacctaa agagactaat aaacaggaga aaaaaacaca tcaagataat
      961 gtaaaatttg tgactaaaga tttcatctgc gggacaatac tcgtttcact aatgctaagc
     1021 tcactatttt gctacatatc aatatcacct gcactattta tgagtgatta tggattaaat
     1081 acatttcact actctgttgt tttttccctg tcagttttgt ttttcatcat tggaaaccaa
     1141 ttgtcaaaaa tcgaaaaaat atcaaaccca tgggtcttat ttcccctaaa tgcaatatca
     1201 gttacacctt ttcttatatt aaatgatgct tttataatat gtgttattgg agcaatgatt
     1261 ttcaatttaa ctcttggcgc atactatcca atagctaatt tcatctcatt gcagataaac
     1321 ggttcacgaa ctggtttagc agcatcaatt acaggcttta ctcaaacagt aagcgcaggg
     1381 acaatcagtt tcctttcagt aaaaataagc gataatggag tttttttggg aaccacttta
     1441 agtattagtt gtcttattct tgcattattg tcaatgttcg taactattat tatagggaat
     1501 aataaaaaat gaattggcaa aagaaaaaga tagaacgttc aatcaaatac caaacaagag
     1561 tatttaacga acatataaat gaactagtga tcaccagaag aaatggtaag gtaatcactg
     1621 ttaatgatgg gagggagatg attgagtttg tgtcatgctc ttatcttgga ctggaaacac
     1681 atgaaaatct aaaacatgca atcgctaatg caattgaaaa atttggagtc caactttcgg
     1741 tcgccagaac gcgcgtaaaa gtagatttat tcgagcaatt ggaaaagaga ttaaataaaa
     1801 taatgaatgg ggcgcacaca ttaacattta atgcggttac tccatgccat tta
//
LOCUS       DAZGBC010000381         1829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGBC010000381 DAZGBC010000000
VERSION     DAZGBC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.383"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(227..1052)
                     /locus_tag="ACHK1J_005249"
                     /pseudo
     CDS             complement(227..1052)
                     /locus_tag="ACHK1J_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1203..>1829)
                     /locus_tag="ACHK1J_005250"
     CDS             complement(1203..>1829)
                     /locus_tag="ACHK1J_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7914365.1"
                     /translation="QKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDY
                     LFNFTGPEGDYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDS
                     WTLTERGRDALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIA
                     RGFRAERIHATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
BASE COUNT          447 a          492 c          386 g          504 t
ORIGIN      
        1 ggcaaaaaaa caatatattg taagcttcat agaatttgga actttatata ataaataatt
       61 ctgagttttt tatcaccatt acattaacaa aattaatgta ttccagagat ttcacattag
      121 tctccaattt ttcagaagaa tctatttacc tggcaggaaa agtcatctgg tgtttcgccg
      181 gagtgagggg tacaggcaat agcggacata accatttgtt ctttttctaa gcagccatct
      241 gataatattt tatcctgaag gctgtcgggg agatattacc cagacgagag tgacgacgct
      301 gacgattgta gaaaatctca atgtattccc gtattactga gatggcctca tcccggcttt
      361 taaaacgata gtggctcaga ctttcatttt tcagcgttcc ccagaagctt tccatcggag
      421 cattgtcgta acagttaact ttacgcgaca ttgatatttt cagaccaaac tgctcctgta
      481 tggccctgta atcgtatgcg cagtactgtg aaccacgatc ggagtggtgg attagcccgg
      541 taggtgagcg ctggttcctg agcgccataa acagggcttt acccgtcaac tatttcgtca
      601 tgcgctctcc catggcgtaa ccgacaattt tgctcgtata aacatctttg acgccagcga
      661 tatacagcca tccttccggg gggcaacata cgttatatct gcaaccaaga cctgattagg
      721 tgctgtaggc gtaaagttct gattcaggag attcggcgca accggcagat tgtgaccaga
      781 gttcgtgttc gccctgaact tgcgtttcag cttacagtgc agcctcagct ccttacgaag
      841 acgtgccagt ctgtcacgac cgacgataat tccgttatct gccagctccg tctggagcag
      901 ctaggttcca tatgtttcgc gtgtgcggat atgtgccacc ttaatctcca gttttagccg
      961 ctcatcactt tgttttctgt ctgagggtca tgctgtactc agtcgtaata accgatcctg
     1021 tatacaccaa gtacccgaca catcgcttca atacataatc gcatctgaat cgttcaccga
     1081 ttcgcggcat ttttcaaggt agttgtcgcc attgttcgac taactgatcg acattacagc
     1141 ctagagccta gctgggggct gtgcacaaaa aagccccgga cattccgggg ctacactgca
     1201 tattaacgaa cgctctgtcc ggttatttca accctgcgat tggggcgcag acattccttc
     1261 agtgcttcgc cctccacatc cgggcagaac accactggtg catcatatcc acggcccgtt
     1321 gcatgtatcc tttcagccct gaatccccgg gcaataaacc atcccctgac tgattccgcc
     1381 cgtttctctg acaggagccg gttatacgcc tgcccgccaa gatggtccgt aaatccttct
     1441 atcttcaccc caacaacatg acccgcctga aacttctctg ccacacggga aagtgcatca
     1501 cgccccctct ctgtcagagt ccatgaatca aaatcaaata atatatcacc ggggactgac
     1561 tccctgtatt ctgctgtctc tgcctccggc agacagtttt ccggcttcca gtagaaagag
     1621 cgggcatcca tgttccggtc aaacagtatc ttgtactggc aggtataatc cccctccggt
     1681 ccggtaaagt tgaacaggta atcccattcc cttacaccag acatcccttc agcaaagtgt
     1741 ggtctccccg ccagctgata aatctggtcc ttattcatac ccgcctttat ctgacgtaac
     1801 ttttctggca gtggccacga acctttctg
//
LOCUS       DAZGBC010000382         1822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGBC010000382 DAZGBC010000000
VERSION     DAZGBC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.384"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1822
                     /locus_tag="ACHK1J_005251"
     CDS             <1..>1822
                     /locus_tag="ACHK1J_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914366.1"
                     /translation="KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAF
                     GCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGR
                     ILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDITAHPAPDRIQVSVLPDG
                     VATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPMSG
                     SLNSVIPPAVQHLTVEVSASDGQYLAQAKWDTPRVVKGVRFSLRLTSGSGENSRLVTS
                     AITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYYQ
                     ITAVPVLAVYDPTVQYEFWFSEKRITDMAQVETFAHYLGTGSQWSVSGPHIKPGKDFW
                     FYVRSVNLVGKSVFVEASGRASNDAEGYLGLFREKIGKLHLAQGMWELIDNSQLDDEM
                     AEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGI
                     PYVAGIGAGIEDADGRPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKR
                     LFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTL"
BASE COUNT          421 a          506 c          571 g          324 t
ORIGIN      
        1 tgaaggaccg gcacacggcg gtggaggtga attacaccga cccgcagaac ggctggcaga
       61 cctccacgga actggtggaa gaccctgaag ccatactgcg ctacggacgc aacctgctga
      121 agatggatgc gttcggctgt accagccgcg gtcaggccca ccgtgccgga ctgtgggtga
      181 taaagaccga actgctggaa acgcagacgg tggatttcac gctcgggtct caggggctgc
      241 ggcacacacc cggtgacatc attgaaatct gtgataacga ctatgccggg accctgaccg
      301 gcggacgcat cctgtccatc gatgccgcca gccgcacact gacgctggac cgtgaggtga
      361 ccctgccgga gacaggtaca tcgacggtga acctgattaa cggcagcggt aagccggtgc
      421 gcgtggacat cactgcacac cccgccccgg accggataca ggtcagcgtc ctgcctgatg
      481 gcgtggcgac atacggtgtg tggggactct ccctgccgtc actgcgtcgt cgcctgttcc
      541 gctgtgtttc catccgggaa aacacggacg gcacctttgc catcacggcg gtgcagcatg
      601 tgccggaaaa agaagctatt gtggataacg gggcccgctt tgagccgatg tccggctcac
      661 tgaacagcgt catcccgccg gcagtgcagc acctcacggt ggaggtgagt gcctcagacg
      721 gccagtatct ggcacaggcg aaatgggaca cgccgcgggt ggtgaagggc gtgcgcttca
      781 gtctgcgcct gaccagtggc agtggtgaaa acagccgcct ggtgaccagc gccatcactg
      841 ccgacacgga gcaccgtttc agtggcctgc cgcccgggga atacaccctg acggtcaggg
      901 cgataaacag ctacggccag cagggcgaac ctgccaccac tacattccgg attaatgcgc
      961 ctgcagcacc ggccagcatt gagctgacgc cggggtatta tcagataacg gcagtaccgg
     1021 tgctggcggt gtatgacccg acggtacagt atgaattctg gttctcagaa aaacgcatca
     1081 cggacatggc acaggtggaa acctttgccc attatctggg gacaggcagc cagtggagtg
     1141 tctccggccc gcacattaag ccggggaagg atttctggtt ttatgtgcgc agcgtcaacc
     1201 tggtggggaa atctgtgttt gtggaagcca gtggccgggc cagcaatgat gcagaagggt
     1261 atctggggct gtttcgggaa aagataggaa aactgcatct ggcacagggg atgtgggagc
     1321 tgattgataa cagccagctt gatgatgaga tggcagagat gaagaccacc atcacagaaa
     1381 cccgcaatga aatcacacag acggtcagta aaaccctgga agaccagagt gccaccatac
     1441 agcagataca gcgggtgcag acagacacaa ataacgacct ggctgcgctg tacatgctga
     1501 aggtgcagaa aacgaaaaac ggcattccgt atgttgccgg tataggtgcg gggattgagg
     1561 atgctgatgg ccggcccctg agcaatatac tgctgcaggc ggaccgtatc gcgatgatta
     1621 acccggagaa cggcaacacc acgccgctgt ttgtggcgca ggggaatcag ctgttcatga
     1681 acgatgtgtt cctgaagcga ctgtttgcag tgagcatcac ctcgtccggc aatcccccga
     1741 cgttctccct gacgccggag ggcaggctga cggcccgcaa tgctgacatc agcggacata
     1801 tcagtgcgaa ctcgggcacg ct
//
LOCUS       DAZGBC010000383         1797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGBC010000383 DAZGBC010000000
VERSION     DAZGBC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.385"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1797
                     /locus_tag="ACHK1J_005252"
     CDS             <1..>1797
                     /locus_tag="ACHK1J_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFO7914367.1"
                     /translation="ITAPDRIGEGKTWIYQRDTMNEREDNKGVHQYGPAEHFTHIISD
                     KPSPKDKYVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLT
                     AVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGP
                     ETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIG
                     GSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPFIIDPVTKERKQIHPEDIHLT
                     AKDFEASKDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRG
                     LRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKAVMNYMEYLVTSQLDE
                     QQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILINI
                     NHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNDDGS
                     TRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNIN
                     HSPHDGLVIINKGNEEVEGTWPNKLQPGKYKN"
BASE COUNT          632 a          355 c          339 g          471 t
ORIGIN      
        1 tattacagcg ccagacagga taggagaagg aaaaacatgg atttatcaaa gagatacaat
       61 gaatgaaaga gaagataaca agggggttca ccagtatggt cctgctgaac atttcaccca
      121 cattatatct gacaaaccct ccccaaaaga taaatatgtt gcatatgcta ttaacattcc
      181 tgactatgag ctggcagccg atgtatataa tattaacgtg acgtcacctt ccggacagca
      241 agaaacattt aaaatactaa tcaatccaga acatctacgg caaacacttg agcgtaaatc
      301 tcttactgct gttcagaaat cacaatgtga aatcatcacc cccaaaaaac ctggcgaagc
      361 gattcttcat gcttttaatg ccacctacca gcaaatcaga gaaaatatgt ctgaatttgc
      421 acgttgccat tatgggtata tacaaatccc tccagtgaca actttccgtg ccgacggacc
      481 agaaactccc gaagaagaaa aaggttactg gtttcacgct tatcaacccg aagatctttg
      541 taccatccac aatccaatgg gagatttgca ggattttatc gcattggtta aagatgctaa
      601 aaaatttggt atcgatatca ttcctgatta tacctttaac tttatgggaa tcggggggag
      661 tggtaaaaat gacctggatt atccctctgc tgatatacga gcgaagatca gtaaagatat
      721 agaaggtggt atccctggct attggcaagg tcaggttttg attccattta ttatcgatcc
      781 agtaacaaaa gaacgtaaac aaatccatcc agaagatata catctcactg caaaagactt
      841 cgaagctagt aaagataata tttctaagga tgaatgggaa aacctccatg cattaaaaga
      901 aaagcgttta aatggaatgc ctaaaacaac ccccaaaagt gaccaggtta ttatgctgca
      961 aaatcaatac gttcgtgaaa tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc
     1021 aaaacactca aaacatgaac aaatagaaag atcaataacc ccaccgctta aaaattataa
     1081 tgagcgatta cacaatacta acttatttaa cccaaaatat catgaaaaag ccgttatgaa
     1141 ttacatggaa tatctggtaa ctagtcagtt ggatgaacaa caaatgtcat cgctgcttta
     1201 tgaaagagat gatttaagcg ccattgattt ttcattgctc atgaagacga taaaagcttt
     1261 ttcatttggt ggcgatctcc aaacccttgc atcaaaaccg ggttcaacaa tctcaagcat
     1321 cccatcaaaa agacggatat tgattaacat taaccacgat tttcctaaca atggcaatct
     1381 tttcaatgac tttctattta accatcaaca agatgaacaa ttagcaatgg catatatggc
     1441 cgctctcccg ttcagcaggc ctttagttta ctgggatggc caagtattaa aatcaacgac
     1501 tgaaattaaa aatgatgatg ggtcgacgcg tgtcggcggt gaggcgtggc ttaataaagg
     1561 ttgctctacc tatcagcagc tctacaatga attccacgca ttatatatag ataaagcagg
     1621 aatatggagc gcatttgagg gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg
     1681 ggattctgtg aacattaatc actctcctca tgatggacta gttataataa ataaaggaaa
     1741 cgaagaagtt gaaggtacct ggcctaacaa attgcaacct ggaaaatata aaaacat
//
LOCUS       DAZGBC010000384         1762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGBC010000384 DAZGBC010000000
VERSION     DAZGBC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.386"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHK1J_005253"
     CDS             <1..315
                     /locus_tag="ACHK1J_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO7914368.1"
                     /translation="RTQSRRLKLSTRRARAAQFRDGWVQGIRQVVMPFSVTEEESRLM
                     RLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNASLHHGVGGTARQMVPGLMIRE
                     AS"
     gene            312..926
                     /locus_tag="ACHK1J_005254"
     CDS             312..926
                     /locus_tag="ACHK1J_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO7914369.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKVDED"
     gene            930..1328
                     /locus_tag="ACHK1J_005255"
     CDS             930..1328
                     /locus_tag="ACHK1J_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914370.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VASCIIEDGCMCVDGVGEYVSHSLPGGTYQLYTASPVPVVPEGLVKAVRFYEQVKREN
                     PPVETGAWKDAVDWVLKEACQAVNIRIKGE"
     gene            1331..>1762
                     /locus_tag="ACHK1J_005256"
     CDS             1331..>1762
                     /locus_tag="ACHK1J_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO7914371.1"
                     /translation="MQISPVTLRVAKAFISRHHRHNKPPVGHKFSIGLRNDAGELTGV
                     ATAGRPVARHFDDGLTLEVNRTCTTGERNANSALYGAVWRAAKDMGYRRCITYTRADE
                     SGASLRAAGFVRVKELPSGKSWAESSVALKSTRDPVGSGGVP"
BASE COUNT          403 a          391 c          578 g          390 t
ORIGIN      
        1 cgtacacaga gccggagact gaaactgagc acccgtcgtg cccgggcggc tcagtttcgt
       61 gatggctggg ttcaggggat tcgtcaggtg gtgatgccat tcagtgtgac agaagaggaa
      121 agccggctga tgcgtctctg gatggaacag caggatatgg tgacggtgga ttcccgggag
      181 atgaaagcct gtcgcggcgg tgacctggcc cgctggcagg ggtatgaggc cgggaaaaat
      241 gcctcactgc atcatggtgt ggggggaact gcccgccaga tggtgccggg tctgatgata
      301 cgggaggcgt catgaataac agcttgtctc aggcaaccaa cggtctgctg atgcaactgg
      361 tgatggacct caagagcggc tacctgcgtc gctgcgaatc tctggggctg gcccgggagg
      421 aaatgcagat gctgcaggga ctgaccattg aggaaattca ttacctgtca aactctgaag
      481 tgtcggttat ccgtctggac attaatcatg aaaacctgct gcgtatgctg cagcaggccc
      541 ggacggagca gaaacgtctg cagcgtattg accgggcact ggcgctggga ggctccattg
      601 agctgatggc gttttacttt ggactttcca gtgtggatgt ggcggcgcgc cggcgcattg
      661 ccggtattga tgttcgcccg ggacgtggca tcacactgag tgatgaggaa agtgcagagc
      721 tgtggcgttt atggcagaag agcggcatca cagatgtgga aagtgcggac ggactggatg
      781 tcatgatgct ggcagctgag caaatgaata tcccgctgac ggcggtatgg catgcagtga
      841 ggggatggca tcagggaact gcggggttta atgatgtatc ggccagtcgc cggaaactga
      901 accgggaaaa agtggatgag gactaaccta tgaccactat taccaaagag cgactgcaat
      961 ggctggctaa catttctggc cgcgatgaca ttgacgatat agacggcggt gagattcgtg
     1021 agctggcgct tatcgcgctg gcatcgctgg aagcagagcc tgtggcgtca tgcattattg
     1081 aagatgggtg catgtgtgtt gacggggtcg gtgagtatgt aagtcactcg ctgcctggtg
     1141 gaacgtacca gctatacacc gcttcacctg tgccggtagt gcctgaagga ctggttaaag
     1201 cggtgcgctt ttatgaacag gtaaagcgtg agaatccgcc agttgaaact ggggcatgga
     1261 aagatgcggt tgactgggtg ctcaaagagg cttgccaggc tgtaaatatt cgcatcaaag
     1321 gagagtgagc gtgcagattt caccagtcac tcttcgtgtt gcgaaggcgt ttatatccag
     1381 acatcaccga cacaataaac ctccggtggg gcataaattc agtattggtc tgagaaatga
     1441 tgcgggtgaa ttgacaggtg tggcgacagc cggcagacct gttgcacgcc actttgacga
     1501 tgggctgaca ctggaggtca atcgtacctg tactactggt gaacgtaacg ctaacagcgc
     1561 actttatggc gctgtctggc gggcggcaaa agatatgggc tatcgccgtt gcattacata
     1621 caccagggct gatgaatccg gagcctctct tcgggcagct ggttttgttc gtgtgaaaga
     1681 acttccttca ggaaaaagct gggcggaatc aagtgtcgcc ctgaagagta cacgcgatcc
     1741 ggttggaagc ggtggtgttc ct
//
LOCUS       DAZGBC010000385         1756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGBC010000385 DAZGBC010000000
VERSION     DAZGBC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.387"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(24..758)
                     /locus_tag="ACHK1J_005257"
     CDS             complement(24..758)
                     /locus_tag="ACHK1J_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO7914372.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYEKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(755..1087)
                     /gene="sctI"
                     /locus_tag="ACHK1J_005258"
     CDS             complement(755..1087)
                     /gene="sctI"
                     /locus_tag="ACHK1J_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO7914373.1"
                     /translation="MSVSNMPPIDRVEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            complement(1107..1346)
                     /locus_tag="ACHK1J_005259"
     CDS             complement(1107..1346)
                     /locus_tag="ACHK1J_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO7914374.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            complement(1360..>1756)
                     /locus_tag="ACHK1J_005260"
     CDS             complement(1360..>1756)
                     /locus_tag="ACHK1J_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO7914375.1"
                     /translation="VSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQA
                     IETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNL
                     KNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
BASE COUNT          521 a          358 c          281 g          596 t
ORIGIN      
        1 agattcataa cgctctcata tgtctactta attttcaacc tgactaaatt agttagaatg
       61 gccctatact tccataacag ccagcaagtc gctacggata ttaatgcaag taagatagaa
      121 accggcatag ccttttcata agcaaaaaca ggttcgctaa tttcatatgt tggtgcttgc
      181 tcaataatgt ctcttcgttt tgacaataca acagaaatat tttcatattg tacgcttgca
      241 aaactattaa caataaatct ctttatgtca tttattttta tttctggatt gatatctttt
      301 tcatatactg caagtacaga aatatgaatt ggtaaagcag ttttaccact atcgccagta
      361 tcaacatcgt aactaacatg tactctcgaa gaaacaatgc catccataat tttgagagat
      421 tgctctaacc gctgctcaat agcagaatat agccttgctt tttccgctcg tggagatgaa
      481 acgagtgcat ctgcagggaa catctgcgat atttgaatat caggcttacc cgggagattg
      541 tagattttta gccaatccac cgcagaagca aaatccgttg gttcgacaaa tattgaaaat
      601 cctgttttgc cttgatcctt cttttcagca ttaatattat gtctttgtaa aacagcaagg
      661 acatcattag cttgctgttg atcaagatgg ttcaataatt cctgctgctt gcagccgcac
      721 aacagcagga taaacaataa taatgatata tatttcatga gcgggtcaac gtctcaactg
      781 cagcgacaat ctttcttgct aatgttccca ccaatgaaac ctcgacatta taattaagca
      841 catgatcctg caagactaat aatttttcag gagttaaccc ctttttatca gaccttaatg
      901 cttgtaacgc attttgctgc caattttcag tttgtacagc atagttggca aaagcggaga
      961 tcattttatc atccgggttg tcaagattga gaacccggtc atttaaatca atcactttcg
     1021 cttgctgtat ttcatgagca gttgattgct caactctatc aataggtggc atattactta
     1081 ctgacataac aaaatcctta tccagtttag cggaagtttt gtatgattgc agaatcgaga
     1141 tctttatacg cttttacaac gttagattgc gcatttcgat ataatgtata ctcagctaat
     1201 gcactttgat attcggcaag gaacttcggg tcggaaggat tggttgctaa atcctccaac
     1261 gctttttcaa cttgctggtt cagatcatca acgccctgat caaattgttt gctgatatcc
     1321 ataatataac cattccaatc tgccataagt cctcctcaat tattcctgaa atgtccactt
     1381 gctaccttga gacttctcga atcggttatc tccgtagcta aatgaactat tatttgtgtc
     1441 ctgatttttc aaattcacat taaatcttac atattgttta ccccattgtt tataatattc
     1501 gtcaacatag ttattaattt tatttaattc actgtcactt aacgtaccaa tgatattgaa
     1561 atacacattg ttagctgttt tatatttttc ataagaaaga cctatcgccg acaacgtttc
     1621 aatagcctga ctttccaata cattatcatc taataccgca atacttatat tgctggcata
     1681 tggcatagtt tgcagtaatc ctttaataag attatcgatt agttgctgtg tgatagcgtt
     1741 ccgttgctta ctcact
//
LOCUS       DAZGBC010000386         1756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGBC010000386 DAZGBC010000000
VERSION     DAZGBC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.388"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            235..684
                     /locus_tag="ACHK1J_005261"
     CDS             235..684
                     /locus_tag="ACHK1J_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914376.1"
                     /translation="MLKKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQNQLS
                     DVKDAAEDVKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKMAEVSEAAQSGLS
                     EVKDAVEEVKTQAAGKVAEVSEATQSGLSDVKDTAPEKGAEISVTEE"
     gene            876..1508
                     /locus_tag="ACHK1J_005262"
     CDS             876..1508
                     /locus_tag="ACHK1J_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7914377.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTLLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
BASE COUNT          421 a          379 c          490 g          466 t
ORIGIN      
        1 tagtgtgttt ctgtgacggg aataaaaaaa tacagcacag ccgatgaccc ggtgctgaaa
       61 acaggttaaa tgtagtgggc acatttccgg taatatcagg aacagcatgt tatgcttata
      121 aaagcatata ccgggagtgt gccctgacac tgtgatgttc tggttgtctt ttgaccattt
      181 tccggggacg aaatataagc tgttctgcgt taacacttaa taatgagaga ttgtatgctg
      241 aaaaaaacaa ttctggcgtt atgtatttcc gctccatttc tggttgcggg ctgtgatgat
      301 gcgacaaagg agaatgtaag taagaaggcg gcagatataa aggaagctgc gcagaatcaa
      361 ctcagtgatg tgaaggatgc cgcggaggac gtgaaaatgc aggctgccgg aaaagtggca
      421 gaggtttctg aagcggctca gtccgggctc agcgaggtga aggatgccgt ggaggaagtg
      481 aaaacacagg cagccggaaa gatggcagag gtctctgaag cggctcagtc cgggctcagc
      541 gaggtgaagg atgccgtgga ggaagtaaaa acacaggcag ccggaaaggt ggcagaggtc
      601 tctgaagcga ctcagtccgg tctcagtgat gtgaaggata ccgctcctga aaagggggct
      661 gaaatatccg taacggagga gtaaaagagc gccgccggtg cagttttttt cagcacccgg
      721 tgacacggaa ttttatatac ggggcatgtc tgtctcttta ataaatcagc atatttaccg
      781 gatatccgat gccctgtacc tgcgctgtca ttatggctct caaacagaca atgctttgtc
      841 tggagcacag gaactctgtc atacctgccg gcacaatgct tctgattgcc gggcatctgc
      901 gccctgcttt tcaggctcat accggacgtt tccttatcgc tgacctcagt gaggaggtgg
      961 tgaggcggta tctgcggttc tgcacgctat tatccggtat ggcagtcagc ccgtcgtctt
     1021 tgcttttaca gatccggcca cagatgtccg tgctggcgga atgtctgatc agacaactta
     1081 ttacagatga accatcgccg gtgtacttca gggatgcggt ggctttttcc agtctggcgc
     1141 tgtttgagga acatccggat ttccatgccc tgctcacggg gtgtatccgg agctgcactg
     1201 tccgggtgaa gaatattatc agtgctgatt tgtcccgtcc atggatgctg ggtgaagtgg
     1261 ccagtctgtt atatataagt gagagtttac tgaagaaaaa gcttcaggaa gagctgacga
     1321 ccttcagtga gcttcttctg aaagagagaa tgcagtatgc cgctcagctt ttgtctgcgg
     1381 gagaaaaccg tatttcccgg gtggcagagt tgtgtggcta tgccagtgct tcttatttta
     1441 tttctgtgtt caggcgttat tatggtatta ccccgaatca gtggcttcct gtgatgcaac
     1501 cggattaatt tgattcagat gcagagtgtg cgttattcag aggaagaggc ttaattttta
     1561 tcatccggtt ttgaatttat tgctgattgt ccctttgcct ttgcgcacgc tgtcagatac
     1621 cagttgtctg acggagtcgc atcatgtacc gactgttact gtattccttc ctgatactac
     1681 cggtggctgc atctgccggt acaacaattt ataccgattc gcaccagcgc cccatgaacc
     1741 cgccagcagg tgttcg
//
LOCUS       DAZGBC010000387         1740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGBC010000387 DAZGBC010000000
VERSION     DAZGBC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.389"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(118..561)
                     /locus_tag="ACHK1J_005263"
     CDS             complement(118..561)
                     /locus_tag="ACHK1J_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7914378.1"
                     /translation="MVISIRRSQHEESEKLIAIWCRSVDATHDFLSAEYRTELEDLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TSNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            718..1647
                     /gene="metA"
                     /locus_tag="ACHK1J_005264"
     CDS             718..1647
                     /gene="metA"
                     /locus_tag="ACHK1J_005264"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO7914379.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          426 a          476 c          391 g          447 t
ORIGIN      
        1 aagcgaaagg ccatcctgac ggatggcctt tttgccgttg gtgcaaaaaa atgccggatg
       61 cgacgctggc gcgtcttatc cagcttacgc aggcacgata gcacgatagg gggcagctta
      121 ttcccccaca tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga
      181 gcgtcccgta accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc
      241 attaacgttg cttgtcagtt ccggggccat tgagagcgca tgcttcacca gcatccggcc
      301 cacgccgcag ccgcgcacat caggatcgat aaacagcgca tccatatgct gcccacttaa
      361 caacataaac ccaaccggct gatcccgttc attaaccgcg acccacaacg gtgcttccgg
      421 cagaaaggaa cgaactaggt cctccagctc ggtccgatac tctgctgata aaaaatcgtg
      481 agtggcatcg acagaacgac accaaatcgc aatcagtttt tcactttctt catgctgcga
      541 gcggcgaata ctaataacca ttttctctcc ttttagtcat tcttatattc taacgcagtc
      601 ttttccttga aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt
      661 atcttcagct atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg
      721 ccgattcgtg tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt
      781 gtgatgacaa cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt
      841 aacctgatgc cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca
      901 cctttgcagg tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc
      961 gcagagcatc tgaacaactt ctactgtaac tttgaagata ttcaggaaca gaactttgac
     1021 ggtctgattg taaccggtgc gccgctgggc ctggtggagt ttaatgatgt cgcttactgg
     1081 ccgcagatca aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc
     1141 tgctgggcgg tacaggccgc gctcaatatc ctctacggca tacctaagca aactcgcacc
     1201 gacaaactct ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt
     1261 ggctttgatg attcattcct ggcaccacat tcgcgctatg ctgactttcc ggcagcgttg
     1321 attcgtgatt ataccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg
     1381 tttgccagca aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa
     1441 acgctggcgc aggaattttt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat
     1501 aactatttcc cgcacaatga tccgcaaaat acaccgcgag cgagctggcg tagtcacggt
     1561 aatttgctgt ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta
     1621 cggcacatga atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag
     1681 caccttacct caggcacctt cgggtgcctt ttttatttcc gaaacacacc tcagtaggtg
//
LOCUS       DAZGBC010000388         1713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGBC010000388 DAZGBC010000000
VERSION     DAZGBC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.390"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..402
                     /locus_tag="ACHK1J_005265"
     CDS             <1..402
                     /locus_tag="ACHK1J_005265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914380.1"
                     /translation="GGVIKVLPVFTLIQTTIFSIPGVALFPAIIGTAILSGIVGSTSG
                     GVGLVMVTFGQDLLELSQAQNISPGLMHRIIVFSASTLDTLPHSGFIITLLGVCGLSH
                     KQSYKELFIVTCVFPAFAVLSMLVYAFLFLI"
     gene            415..>1713
                     /locus_tag="ACHK1J_005266"
     CDS             415..>1713
                     /locus_tag="ACHK1J_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7914381.1"
                     /translation="MSIYSRKQCTLEDAVNMIHDNDCVILGGFIGAVVPECIEKGIED
                     RFLKTGKPRNLKIIFAAGQGDAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGK
                     ITGYNLPQGVICHLFRDCAAGKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEI
                     NGVENLFYKKIDANIAILRGTTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQV
                     KQIVRDGTLDPLQVRIPGIYVDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETR
                     PLDIKKIIARRAAMELKKNAVVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGTP
                     AGGLSFGASSFPQAIITQDQMFDFYDGGGLDQAFLGLAETDEKGDLNVSKFGTKIAGC
                     GGFINITQNAKKVYFCGSFTAGKNKIYVSNGKLHIKEDGSV"
BASE COUNT          525 a          256 c          396 g          536 t
ORIGIN      
        1 ggtggggtaa ttaaagtcct tcctgtgttt acccttattc aaacaacaat tttttctatt
       61 cctggagtag cattatttcc tgcaattata ggtacagcaa ttctttcggg aatagtgggc
      121 tcaacatcag gaggggtggg attagttatg gtaacgtttg gtcaagactt attagaacta
      181 tctcaagctc agaatatttc gccaggttta atgcatcgta ttattgtttt tagtgcatca
      241 acgctggata cgcttcctca ttctggcttt attatcacat tgctaggagt ttgtggctta
      301 tctcataagc aaagttataa agagcttttt attgttactt gtgtgtttcc cgcatttgcg
      361 gttctctcca tgttggtata tgcattctta tttttaattt aaaagaggtt taatatgtct
      421 atttattcaa gaaaacaatg tacactagaa gatgccgtaa atatgattca tgataatgat
      481 tgtgttattc ttggcggatt tattggtgca gttgttcctg agtgtataga aaaaggcata
      541 gaagatcgtt ttttgaaaac gggaaaacca agaaatctca agattatttt tgctgctggt
      601 caaggggatg caggaacaaa agctgtcaat catttatctc atgaggggct tgtcactagt
      661 gccattggcg ggcattgggg actaatacct aaattgcaga agttagctaa tgagggaaaa
      721 attacgggtt ataatcttcc tcaaggggtt atttgccatt tattccgaga ttgtgctgct
      781 gggaagttag ggacattaac tcatgttggt ttaggtacat ttgttgatcc ccgcgtagag
      841 ggcgggaaaa ttaatgataa aacacaagag gatattgtgt ccgtcacaga aattaatggt
      901 gtggaaaacc tattttataa gaagattgat gctaacatag caatcctcag aggtactaca
      961 tctgatcaga gtgggaatat aacaatggag gatgaatgtt tgattcttga aaacctagct
     1021 gcagcccagt tagtccataa taatggaggg aaagttattg ttcaggtaaa acaaatagta
     1081 cgtgacggta cattagatcc tttacaagtc agaattccag gtatttatgt tgatgctgtt
     1141 gttgtttctg aagatgaata tcatatgcaa acttttgcgg agtatatgaa cttcagctat
     1201 gtcgggaaag gtgtaaaaga ggtaaaagac aaagaaacac gccctcttga tataaaaaaa
     1261 attatagctc gacgagccgc aatggaactg aaaaaaaatg cagttgtaaa ttatggcata
     1321 ggggcacctg aagttatagc tgaagtaaca gatgaagagg ggattacctc cgaacttatc
     1381 gcaactgttg aaccaggtgc aattggagga acgcctgctg gaggtctgtc atttggagct
     1441 tcttcgttcc ctcaggcgat aattactcag gatcagatgt ttgatttcta tgacggtggt
     1501 gggcttgatc aagcgtttct tggattagct gaaacagatg aaaaaggaga tttaaatgtt
     1561 tctaaatttg gaacaaaaat tgcagggtgc ggaggattta ttaacataac tcaaaatgct
     1621 aaaaaagtat atttctgcgg ttctttcact gctggaaaaa ataagattta tgtcagcaat
     1681 gggaaattgc atataaaaga ggatgggagt gta
//
LOCUS       DAZGBC010000389         1705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGBC010000389 DAZGBC010000000
VERSION     DAZGBC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.391"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..165)
                     /locus_tag="ACHK1J_005267"
     CDS             complement(55..165)
                     /locus_tag="ACHK1J_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914382.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            complement(218..622)
                     /locus_tag="ACHK1J_005268"
     CDS             complement(218..622)
                     /locus_tag="ACHK1J_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO7914383.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(843..1574)
                     /gene="bluR"
                     /locus_tag="ACHK1J_005269"
     CDS             complement(843..1574)
                     /gene="bluR"
                     /locus_tag="ACHK1J_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO7914384.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          476 a          357 c          350 g          522 t
ORIGIN      
        1 ttgtttttat aaatatatta accgttataa aataacgtat agaaagtcaa gtgatcacat
       61 ttcaaatatc aatttgatag tattggcctg gtgattattt atgggtagca ataaaaggac
      121 agtatttatc atccataggg atagtctctg tacttttatt cccattatgc taatgcctta
      181 ctgaattatg aagcatttct taagcatcca actttagcta gattaatggt ttattatttt
      241 ctacatcttc aatatataaa agcgtattat caatggcgta gtaactgcgt ttgttatgat
      301 taacatcagt aacccaacgg aaaacgcccg cgcctgccag tgttgaacag tattcccgaa
      361 atgtagattt tccgcaaata tgaagcaatg cggcctcttt tattttagca gggttcttgg
      421 tcgtactaac ttttaacagg ttcctggttc ctcttaataa caaaaccgtg tcatcgtgag
      481 taataattct gatgttatcc gtagccagat aataaatgta atgtgcaata cggtgatgtt
      541 ttaattctga ataaaaccag gagaagtttt gctcttttct cacttgctca aacatctttt
      601 gaaaaacaac gacctgatcc atcaggataa taacctcttg ttagttgtga gactgcgtag
      661 tgtgcacgat cggttttacc acttcaatct ggtctgtcct ttggctgtga tatgtacaaa
      721 gtgtgaaagg gggattacct gaattctccc ggtgagcatt ttgtaatgga ccagctccgg
      781 tacaaacgct gttgtgggtt cagattataa cattctgtct aaggggcggg ataaaggtga
      841 aattaggggg catgaaagat gactttataa ccttgctcac cccagtgttg taaaagttcg
      901 ttttgccttc tcgttggtgc catgcctgtc cagacaatca atgtttgcgt cgggaacagt
      961 tccgggcgcg gtgattcaat gggatcagca agaacagaaa tatgccatcc tgacaatgat
     1021 aaacgccatg cttccagcca caatcgagct ctctcttcaa cattccacgc catcagaatg
     1081 gcttctttac cgggcttacg gcgcatttcg aaaagcgagg ctgctgcata ttcaattaat
     1141 gcgccgtcaa acatactgct cataatgcgg gaggtgttgt gatcaagcac gagacgctgg
     1201 cgaacaggaa ggtaaacatg attaatcaat tgatctactg ggtactctcg acccagtgaa
     1261 ataattctcg cgcgtagttt ggcaggatta gccatacgaa gaattgacat catctcttct
     1321 tgcaggcggc tccagtcatc ttccgtatcc tggctggtgg tttccagtaa tgctttaact
     1381 ttgcctacag ggacgccatt acttatccaa cgcttgatct cttcgatacg ttgtatgtct
     1441 tcttcatcaa agagtcggtg tccgccttca ctgcgctgtg gttttaacaa accgtagcgg
     1501 cgttgccagg cccggagagt gacaggatta atcccgcaac gttcagcaac atcaccaatg
     1561 ctgtaataag ccacaattcc tccttgcggt cacaaatctc cgtcgcctgt aaacgaccca
     1621 ataatacttt gtacaatata cgctaaaatt gtacaaagta taaataagat ttcagctaat
     1681 ttggatgaaa cattattttt aatgt
//
LOCUS       DAZGBC010000390         1689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGBC010000390 DAZGBC010000000
VERSION     DAZGBC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.392"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1629
                     /locus_tag="ACHK1J_005270"
     CDS             <1..1629
                     /locus_tag="ACHK1J_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO7914385.1"
                     /translation="TSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYS
                     VDGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLI
                     VEYDIKVRMKLPAQRAALEKLRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPV
                     SPKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPD
                     TPEEEPPGKLRPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELV
                     KNHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHILEF
                     PMKTFSQVMSQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNA
                     PNELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINA
                     LTDNGLQFAGYGEAVNHDENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRS
                     LGFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGL"
BASE COUNT          466 a          510 c          423 g          290 t
ORIGIN      
        1 acatcgtcag cggaaccgga cacgattatc tttgcacccc gccgtcccga cagtccgcag
       61 cggcacaacc tggacgagat ccttaaaaat cttacacaca aagagtttcc cgacaggact
      121 gcgctctaca gcgttgacgg tgaacccgcc ttcctggaca gactctactg tctcgaaatg
      181 gtggatggcg ccagcgcgga tgacaaaaag gtcctggctg cccttgctgt ggccaccaac
      241 ttctatggcg gcgttatcga gctgaccggc agtgatgcct ttaaacaaaa agccatgcag
      301 ctgattgtcg aatatgacat caaggttcgc atgaaactcc cggcccagcg ggcagcactg
      361 gaaaaactcc gcaaggaaaa agagatggcg catgatgcca ttgtcaccca cagacccacg
      421 ccggaactca accgccagtc accggaagag acaacaatgg cccaaccggc tccccctgtg
      481 tcaccgaagg cagaggctga acaaacagca ggcagtacgg ctgaaacacc gcatccggaa
      541 gcgccggtca cggcaaaagc tgaagccccc cgggccgaca gaccggaaca acccgccgcc
      601 ccgtcgtctg cgccagaaaa agcccctgtc agtgcacagc cgcagcaacc cgacacaccg
      661 gaggaagagc cgcccggcaa gctcagaccc ggtgaaagtg tgactgccgt cctgacaaac
      721 tacggagaaa aagagtatgc gcccggaaaa gggcgctgct tttttgtgga gctcaccaac
      781 cgcagtggca aacgggaata ctggggaaac ggcctggaag aactggtcaa aaaccatcag
      841 aagggtgacc ccgtcacact gacactgcag gcgagagaaa cattccggct gtccggtgaa
      901 acgcgggatc gtatccgtaa tatctgggcc ataaccccgg tcagtaacgg cattaccgtt
      961 aataatgacc aacctcatgc gggtcagcat atcctggaat ttccgatgaa aacattcagt
     1021 caggtaatgt cacaaatgca acaacactgg ccacagataa tgcaggaagt tcgttccccc
     1081 aaaaatcctc ccaataatct gtacattggt gaagacaggc aacctgcatc agcacctcag
     1141 gatcaatcaa tgcgactccc cctcgctggt aatgccccca atgaactgat accagccatc
     1201 gcctctgccg ataaggacaa tcgtcagttg aatcttctgc tggtccacag cgcagatgat
     1261 catctgcagg gcgtggtccg cctgaacggc accctttacc cggcactggc caccccgtca
     1321 gctgacaacc gtcagctggt catcaatgcc ctgacggata acggtctgca gtttgccggt
     1381 tacggtgaag ctgttaacca tgatgagaat actcatcaac gtccgtcccc acaaataatg
     1441 cagtttcacc tgaagcaaca ggactccccg ttgtttgccg ccatccacaa accggaagag
     1501 caaccggaca agcttttccg ctctctgggt tttgagcaga cctggaaaga gtggtctgac
     1561 agccaaaagg cggaggacag gcaggaaaaa actctgcaac aggcacaatc ccacagcccc
     1621 ggcctgtgag ccggtaagga cgaccatgaa catgaaaaaa acgctgctgg tcatactgac
     1681 cagcatctc
//
LOCUS       DAZGBC010000391         1671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGBC010000391 DAZGBC010000000
VERSION     DAZGBC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.393"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1671)
                     /gene="espI"
                     /locus_tag="ACHK1J_005271"
     CDS             complement(<1..>1671)
                     /gene="espI"
                     /locus_tag="ACHK1J_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFO7914386.1"
                     /translation="KKVTGKTGRRLMTVSLVLSVTLSVLPGKASTVSAEIPYQTFRDF
                     AENKGVFTPGVTGIEINDNNGNKVGVLDVPMLDFSSLSRDGHTTLIHPGYVVSAKHGG
                     LQSVSSATFGYDQIYKIVDNNLAGIDFSAPRLNKLVTEVIPADIQGKDKFNNNRYTAF
                     YRAGVGSQYIRYANGTDKLLQAYTPEKAYLTGGTVGKPYYTHYNGMKMISANPGNTFD
                     KNQRPLASYGQSGDSGSPLYAWDNIDKKWVLAGVTLHNYGVKGARNDWLLIPHDFISQ
                     KLQDDLKPIIVASPEENILRWEFDRSRGTGTLSQGEKIFSMTGSVNGNANTGNNLVFS
                     GNEGKIELVSSVEQGAGYLQFDKDYTVLTNNNSTWTGAGIIVGDEANVKWGVNGIAGD
                     NLHKVGSGTLTVNGHGENKGGLKVGDGVVVLEQQPDANQKQQAFSHINIASGRATVKL
                     NGANQVDADNISWGYRGGKLDLNGYDFTFSRLQAADYGAEISNDNQTDKSIVTLSLSP
                     LKAEEINVVVNNINIMGGTGKPGDLYYTTFDGNYYLLKSNRYGSALFGA"
BASE COUNT          462 a          400 c          294 g          515 t
ORIGIN      
        1 gcgccaaaca aagcgctgcc atatcggtta cttttcaaca gataataatt tccgtcaaag
       61 gtcgtataat acagatcacc tggtttgcct gtccccccca ttatatttat attattaaca
      121 accacattta tttcttctgc tttcagagga gataacgaaa gtgtgactat ggatttatct
      181 gtctgattat cgttgctgat ttcagcacca taatctgcag cctgaagacg ggaaaaggta
      241 aaatcatacc catttaaatc cagtttcccc ccccgatatc cccagctgat attatctgca
      301 tctacctggt ttgccccgtt aagtttaact gttgcccgac cactggctat attgatatgg
      361 ctaaacgcct gttgtttctg gtttgcatct ggctgttgct caagaacaac aacgccgtcc
      421 ccaactttaa ggccaccttt attctcacca tgaccattaa cagttaatgt cccggaacca
      481 actttatgca gattatcacc ggcaatgcca ttaactcccc atttgacatt tgcctcgtca
      541 ccgacaataa ttccggcacc agtccatgta ctgttattat ttgtcagtac agtgtagtct
      601 ttatcaaact gaagatatcc ggctccctgc tccacactgg ataccagttc gattttccct
      661 tcattacctg agaacacaag attgttcccg gtatttgcat ttccgtttac actaccagtc
      721 atggaaaaaa tcttctctcc ctgactgaga gtacctgtac ctctcgaacg atcaaattcc
      781 cagcgtaaga tattctcctc cggagaagca acaataattg gtttaaggtc atcctgtaat
      841 ttttgactga taaagtcatg aggtatcagg agccagtcat ttcgtgcacc ttttactcca
      901 taattatgca gagtaactcc agctaatacc cattttttgt caatgttatc ccaggcatat
      961 aacggcgaac cactgtctcc actctgtcca taactggcaa gaggtctctg gtttttatca
     1021 aaggtatttc ccgggtttgc tgaaatcatc ttcataccgt tatagtgagt ataataaggt
     1081 ttccccactg ttccgccggt cagataagcc ttttctggag tgtaagcctg cagtagttta
     1141 tctgtgccat ttgcataacg gatatattga gagccaacgc ccgcacggta aaaagccgta
     1201 taccggttat tattaaattt atcctttccc tgtatatctg cgggaattac ttctgtaaca
     1261 agcttattta atcgtggggc agaaaaatct ataccagcaa ggttattatc aactatttta
     1321 tatatctggt cataaccaaa agttgctgat gaaacacttt gtaaaccacc atgtttagcc
     1381 gatacaacat aaccaggatg aatcaatgtg gtatgaccat cacgagaaag actggaaaaa
     1441 tcaagcatgg gaacatcaag aaccccaact ttatttccat tgttgtcgtt tatttcaatt
     1501 cctgtcactc cgggggtaaa cacaccttta ttttcagcaa agtcacgaaa agtctgatat
     1561 ggtatttctg cgctgaccgt tgatgcttta cccggtaaaa cagaaagagt cactgataat
     1621 accagggaaa ccgtcattaa tcttctgccg gtttttcctg tgactttctt a
//
LOCUS       DAZGBC010000392         1666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGBC010000392 DAZGBC010000000
VERSION     DAZGBC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.394"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..824
                     /locus_tag="ACHK1J_005272"
     CDS             <1..824
                     /locus_tag="ACHK1J_005272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HFO7914387.1"
                     /translation="SNTGVSMELFAITDNTVGTRIVRIVTDRPTQNVVTQLFSEQKTF
                     FEERYTEGVEFSGGYITSGDEFFVIPDFDDVIAVLDAINNPTSIPPWEPEEISAFNII
                     ALFSGYPEEDGKPVTALIQSFDKRQVIDNRRTILQKMFQASNTFCQSTEHGIVIDNKL
                     SAILAGTELKFKSFHMLRRIFDVDAYFREATNEELTSFSSHEKFSVVPGFDLTTIADS
                     VIRKKVSLINKSRILEDYSVTELRISASEIGVALEAEKIGEIEKIKMPQIRKDVN"
     gene            1353..>1666
                     /gene="subA"
                     /locus_tag="ACHK1J_005273"
     CDS             1353..>1666
                     /gene="subA"
                     /locus_tag="ACHK1J_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO7914388.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHR
                     VYG"
BASE COUNT          514 a          300 c          337 g          515 t
ORIGIN      
        1 atagtaatac aggagtaagt atggaattat ttgcaataac agataataca gttggcacac
       61 gtattgtcag aattgttact gacagaccga ctcaaaatgt agtcacacag ttattcagtg
      121 aacaaaaaac attttttgaa gaacgctaca cagaaggtgt ggaattttca ggtggataca
      181 tcactagtgg agatgagttt tttgtcattc ctgactttga tgatgttatc gctgtattag
      241 atgccattaa taatcccaca tctatccctc catgggagcc ggaagaaata tcagccttca
      301 atataatcgc tcttttttca ggctatcctg aagaggatgg aaaacctgta actgcattaa
      361 ttcaaagctt cgataaaaga caagttattg ataacagaag gacaatttta caaaaaatgt
      421 ttcaggcaag caatactttt tgccagtcta ctgagcatgg tatagtcatt gacaataagc
      481 taagcgctat tctcgcaggt acagaattaa aatttaaaag tttccatatg ctccgcagaa
      541 tatttgatgt agatgcttat ttcagagagg cgactaatga agaattaaca agtttttctt
      601 ctcatgaaaa attttcagta gttccagggt ttgatttaac aacaattgct gactctgtta
      661 ttagaaagaa agtttcctta attaataaat caagaatact tgaagactac tctgtgactg
      721 agttaagaat ttcagcgtct gaaattggtg ttgctctaga agctgaaaaa ataggtgaaa
      781 ttgaaaaaat aaaaatgcca caaataagaa aagatgtaaa ttaaggaaac aacatgttgg
      841 gtggagcgga caatccaaat ggcgaattac cgtcttatat cactggcgct gacacctgat
      901 atccggggct tactgcacac ataataatga gccagggtaa acgacacaac catgcgctga
      961 tcgctccggt aagacggcac tgtaatttcc tgttcgccat gatgcatgac ggtactcttt
     1021 attgtcataa acatgccagg caacatagtt gccccctctt attttttctg gctaattttt
     1081 acccatcata gctggattta cttctgctct gaaggagtag gaagagcatc tcaccgatta
     1141 cctattgcag aaatcctggt gcatattacc gtgaaggagt tcagtgagct attgaatatg
     1201 caaataaata aattaatatt tttgagaatg acttcactcg ctttattgat tgtgattgtt
     1261 tatcttttgt cgaaaacagg atatttcaga tgtgatgttc ctgtaagata ttgacctgca
     1321 atactgttta tttgtactga ctggaggata ttgtgcttaa gattttatgg ccgcatattc
     1381 tgttcctgct ttttatatct gtgtctgtca gggcagaaaa accctggtat tttgatgcaa
     1441 ttggcctgac agaaacaacc atgtcgctca cagacaagaa taccccggtt gtcgtatctg
     1501 ttgttgattc cggagtggca tttgtaggag gcctcagcga cagcgaattt gccaaattca
     1561 gttttactca ggatggttcg ccattcccgg taaaagagtc tgaagcttta tatattcatg
     1621 gtactgccat ggcttcactc attgcctcac gtcatagggt ttatgg
//
LOCUS       DAZGBC010000393         1646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGBC010000393 DAZGBC010000000
VERSION     DAZGBC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.395"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..511
                     /locus_tag="ACHK1J_005274"
     CDS             <1..511
                     /locus_tag="ACHK1J_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7914389.1"
                     /translation="ETASIFAEKLCEAKAMVSHGHFFGKIRAQREACGLTTTELAKLL
                     DLDEEIILQWESGEFEPTISMLIPLANVIGCDPLSLLDEKDSASVIRVNAPEVYVESI
                     GARIKSARKKLGLTEADLARMINTYSDPINDWECGICEVPADQIVPLASALNCDLMWL
                     LTGKSEAKE"
     gene            517..717
                     /locus_tag="ACHK1J_005275"
     CDS             517..717
                     /locus_tag="ACHK1J_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914390.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHDLLCAVVVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            916..1098
                     /locus_tag="ACHK1J_005276"
     CDS             916..1098
                     /locus_tag="ACHK1J_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914391.1"
                     /translation="MHQKTAEHEQTRVLLTIKNGKVILIRHVHDDEFVGNLSTFLFIA
                     EKAGYDVIAPADEDEE"
     gene            1104..>1646
                     /locus_tag="ACHK1J_005277"
     CDS             1104..>1646
                     /locus_tag="ACHK1J_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914392.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPSPIRFALQQYIEWNLIQTYGEAEGMGRALFLYQKMLTTSPDKGF
                     ILSDIGREGLELLLDEMINDL"
BASE COUNT          520 a          339 c          375 g          412 t
ORIGIN      
        1 agaaactgca tcaatttttg cagaaaaact atgtgaggca aaagctatgg tttcgcatgg
       61 tcactttttt ggaaaaatcc gtgcccaacg tgaagcctgc ggcttgacga ccaccgaact
      121 tgccaaactg cttgatctcg atgaagagat catcctgcaa tgggagagcg gagagttcga
      181 accgaccatc agcatgctta tccccctggc aaacgtcatt gggtgcgatc cgctttcttt
      241 gctggatgaa aaagatagcg cgtctgttat tcgcgtaaat gcgcctgaag tctacgtgga
      301 aagtattggc gcacgcatca aaagcgcacg taaaaaactg ggcttaacgg aagctgatct
      361 tgcccgcatg attaatacct atagcgaccc cataaacgac tgggaatgcg gcatctgtga
      421 agttcctgct gatcagatag tgccactggc cagtgcgctt aattgtgacc tgatgtggtt
      481 gttaacggga aaatcagaag caaaggagta gcaacaatga ctggtaatat ccatgataag
      541 tatgaaggct tatgcctagc accagattcc ttcgcaaaca atatccatga tttattatgc
      601 gcagttgtcg tattacaaat gtcagacaac gacgcaataa aaagaacagg tgatgaagtt
      661 cttgaatttg cacgttgcta tgctgaagca gcagctgaaa aagaactatc cagttaaata
      721 gaacaagtca tctccagata ctatattacg gcttaatcgc cggggattat cacacactta
      781 atccacagga ggttttatta tgacttttat aaaacataag gcatcacaca aaacagcctg
      841 ccttattgca caacacggga aaaattacat gcatattgcc tgcttgtttc tgcgtaaagc
      901 atacgggaga taataatgca tcagaaaaca gcagaacacg aacaaaccag agtattgctg
      961 accatcaaaa acgggaaagt aatattaatt cgtcatgttc atgacgatga atttgtagga
     1021 aatctttcaa cattcctgtt tattgcagaa aaggcaggat atgacgttat tgcaccagca
     1081 gatgaagatg aggaataaat ttcatgcaat acgctgaatt ccaggctgaa gcaacagcca
     1141 atggtataca aacaggcagt atgactattg attatcacga cgccatacgc cgtctggatg
     1201 ctggagaatt cgatactcct aatgtgcgag gtttacgtat ccttcagtgt ctcgcgcaag
     1261 ctgacgaagc aggattactg ggaaaacttc cggttgagat gaaggttgcc cagtggcgat
     1321 ggttgtatgt gacgacattc atcaacgaag aagaagacaa gaacggcaca attgatgtcc
     1381 cgaatgaaca cggaacaaca gatcgcgccg taatatataa cgggaagcat ggagttatga
     1441 ctatataccc tagtcccatt cggtttgcct tacagcagta tattgaatgg aatttaattc
     1501 aaacatatgg cgaagctgag ggaatgggaa gagcgctgtt tctttatcag aaaatgctca
     1561 ccacttcccc tgataaaggt ttcatccttt cagatatagg tcgagaaggg cttgaactcc
     1621 ttctggatga aatgattaac gacctg
//
LOCUS       DAZGBC010000394         1643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGBC010000394 DAZGBC010000000
VERSION     DAZGBC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.396"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..580)
                     /locus_tag="ACHK1J_005278"
     CDS             complement(<1..580)
                     /locus_tag="ACHK1J_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO7914393.1"
                     /translation="MNKVYSLKYSHITGGLIAVSELTRRITTGSRKKLFAIISLSLIG
                     VITPSVASQMDLTNFYSQDFFDFAQNKGAFKTGAKNITILKKDGSTLELPDVPFPDFS
                     SVSNKGSTTAIGGSYTVTATHNNISHHSISTQGFGQTNYSYIDRMSKGDFAVTRVDKF
                     IVEGTGDIESADISLSKNEALERYGIMYKGKKQ"
     gene            complement(1431..>1643)
                     /locus_tag="ACHK1J_005279"
     CDS             complement(1431..>1643)
                     /locus_tag="ACHK1J_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO7914394.1"
                     /translation="GRVYTEDGMEVNRWLVQHGAAWVYPDFNTDYTLPAYQREARTMK
                     QGLWADKKPVPPWLWRKENKIAQDCK"
BASE COUNT          434 a          307 c          351 g          551 t
ORIGIN      
        1 gttgtttttt ccctttatac ataatcccat atcgttcaag agcttcgttt ttagataaac
       61 tgatatcagc actctctata tctccagtgc cttcaacaat aaatttgtcc actcgtgtaa
      121 cggcaaagtc acctttactc attctgtcta tatatgaata gtttgtttgc ccaaacccct
      181 gggttgatat agagtgatga gatatattat tgtgggttgc agtaacagta tatgaaccac
      241 caattgcagt agttgacccc ttattcgata cagatgaaaa atcagggaat ggtacatctg
      301 gtaattcaag ggtagagcca tcttttttca gaatagtgat gttttttgct cctgttttga
      361 atgctccttt attttgtgca aaatcgaaga aatcctgcga ataaaaattt gtaaggtcca
      421 tttgagatgc aactgaggga gttattacac caatcagaga taatgaaata atagcaaata
      481 gtttttttct ggatcctgtc gttattctgc gcgttaattc agatactgcg attaatcctc
      541 cggtaatatg actgtattta agagaatata ctttattcat ataagctcca gtatgttagg
      601 aaaagtatta ttgttgttaa tctgttagag ggggactctg acagttttat gattgtaatc
      661 atttttcaga caaatgttta gcatttggtg agtttgttat gatgcagata acaaatacat
      721 catgtccatt atggattctt cagcgggaac tcagcaaaaa aaaggagggg cggtagtttt
      781 ttatcatgtt ttttattttt taaagtatgc ctggtaccgg gaaggataaa gcatacagat
      841 gcgacattga ttaatatttc ttattaattc agggggggag tattctgatg cctttgtgtg
      901 ggatagttct catgttgtat gccaaaagtg ttttgtagat ggcatgcagt agaagttccc
      961 tggcgatgtt ttatcgttca atccggaact ctgctgtaat gtcaggacaa catcatagta
     1021 gccaggtata acagttgaaa ggcaatggat ggattgtgat gccacaacga acgctttgcg
     1081 gattctgttt cctccaggga aatatggtgc ctatggcaac gttagctcac aaagtttgtc
     1141 aatgtcacga tctttgacag ttgccatttt tactaagatc aacgttaaca ggaaacacgg
     1201 tagaaggaaa tatgctgtcg gattgttgcg gaagctgatc taaccttaca tctccctgtt
     1261 tcctgtatta tttaaagcag ggcaagtgta tttaacgttt ccggattctt ccgcactatc
     1321 cggatcagta aaacagcctg agcgttaggt ttggccctgc tttgctcccg cttctggtac
     1381 gtggtttcac ctgtatggcg atagtccgca aaaactgcct gtgagctgtt ttatttacaa
     1441 tcctgtgcaa ttttattttc ttttcgccac agccatggcg gaacaggttt tttgtctgcc
     1501 cacagaccct gtttcattgt tctggcttct ctctggtatg ccggcagggt gtaatctgtg
     1561 ttgaaatcag gatataccca ggctgcacca tgctgtacca gccagcggtt aacttccatg
     1621 ccgtcttcgg tatatacccg tcc
//
LOCUS       DAZGBC010000395         1638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGBC010000395 DAZGBC010000000
VERSION     DAZGBC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.397"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..1444
                     /gene="gndA"
                     /locus_tag="ACHK1J_005280"
     CDS             38..1444
                     /gene="gndA"
                     /locus_tag="ACHK1J_005280"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO7914395.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     DNPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGQPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAGNPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLN"
BASE COUNT          426 a          379 c          401 g          432 t
ORIGIN      
        1 atatcaatta atgactaacc cctgacagga gtaaacaatg tcaaagcaac agattggcgt
       61 cgtcggtatg gcagtgatgg ggcgcaacct tgcgctcaat atcgaaagcc gtggttatac
      121 cgtctctatt ttcaaccgtt cccgtgaaaa gacggaagaa gtgattgccg acaatccagg
      181 caagaaactg gttccttact atacggtgaa agagtttgtt gaatctctgg aaacgcctcg
      241 tcgcatcctg ttaatggtga aagcaggtgc aggcacggat gctgctattg attctctcaa
      301 gccatacctc gataaaggcg acatcatcat tgatggtggt aacaccttct tccaggacac
      361 catccgtcgt aaccgtgagc tttctgcaga aggctttaac ttcatcggta ccggtgtttc
      421 cggaggcgaa gaaggtgcgc tgaaaggtcc ttccattatg cctggcgggc agaaagaagc
      481 ctatgaactg gttgctccga tcctgaccaa aatcgccgcc gttgctgaag atggccaacc
      541 gtgtgttacc tatattggtg ccgatggcgc gggtcactat gtgaagatgg ttcacaacgg
      601 tattgaatac ggtgatatgc aactgattgc tgaagcctat tctctcctga aaggcggcct
      661 gaatctctct aacgaagaac tggcacagac ctttaccgag tggaataacg gtgaactgag
      721 tagctacctg attgacatca ccaaagatat cttcactaaa aaagatgaag atggtaacta
      781 tctggttgat gtgattctgg atgaagcagc taacaaaggc accggtaaat ggaccagcca
      841 gagtgcgctg gatctcggcg agccgctgtc gttgattacc gagtctgtgt ttgctcgtta
      901 tatctcttct ctgaaagagc agcgtgttgc cgcgtctaaa gttctgtctg gtccgcaagc
      961 gcagccagca ggcgacaagg ctgagttcat cgaaaaagtt cgtcgtgcgc tgtatctggg
     1021 caaaatcgtt tcttacgctc agggcttctc gcagctgcgt gctgcgtctg aagagtacaa
     1081 ctgggatctg aactacggcg aaatcgcgaa gattttccgt gctggttgca tcatccgtgc
     1141 gcagttcctg cagaaaatca ccgatgcata tgccggaaat ccgcagatcg ctaacctgct
     1201 gctggctccg tacttcaaac aaattgccga tgactaccag caggcgctgc gtgacgtcgt
     1261 tgcttatgca gtacagaacg gtatcccggt tccgaccttc gccgctgcgg ttgcctatta
     1321 cgatagctac cgtgccgctg ttcttcctgc gaacttgatc caggcacagc gtgactattt
     1381 cggtgcacat acttataagc gcattgataa agaaggtgtg ttccataccg aatggctgaa
     1441 ctaatcttaa tttagatatc tcgcccggat tacaccgggc ttttcaatgc ctatagaaaa
     1501 ccatcagata ggcttgtagt ttagttaagc ttacagcttt cggggaatta taaaaatttt
     1561 tattttatgt catcaataaa atagaagtgt aaatcatgaa atatagtgtc attaattaaa
     1621 tgcgtacttc tcatttat
//
LOCUS       DAZGBC010000396         1626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGBC010000396 DAZGBC010000000
VERSION     DAZGBC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.398"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..351
                     /locus_tag="ACHK1J_005281"
     CDS             <1..351
                     /locus_tag="ACHK1J_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914396.1"
                     /translation="VYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQWGS
                     IEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPHAIKMYTLSNPSLVSVTLITV
                     RGCYGKFSWEAIGY"
     gene            361..585
                     /locus_tag="ACHK1J_005282"
     CDS             361..585
                     /locus_tag="ACHK1J_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914397.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            585..1256
                     /locus_tag="ACHK1J_005283"
     CDS             585..1256
                     /locus_tag="ACHK1J_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO7914398.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
BASE COUNT          426 a          327 c          512 g          361 t
ORIGIN      
        1 gtttacggag acacacaacg ttttcactgg cgggtggatt acagcacaaa aaacaagccc
       61 acaacagcaa gcctgacagt aaatggctgg gagcgtgatg aagtaaccgg tcgtttgcgg
      121 caatggggca gtattgaggt aagtgaggat gatggtaagc ttatgacaca gcactctatc
      181 cgttttcctg tcgcttttcc ggttgctgct ctgaatgctc aggtgtctgc agtgggagac
      241 ccccacgcca ttaagatgta taccctgtcg aatccgtcat tagtgtctgt gactctgata
      301 acagtcaggg gatgctacgg caagttttca tgggaagcga tcggatactg agggcattgt
      361 atggaacagt atgttttttc accgtcagag aatatgttct atcccctgtc cctgcggccg
      421 gtttatgagg cagcagggcg ctggccggaa gacggtattg tggtggatta tgtggtgtat
      481 aaagtctttg cagcggatgc tgcaccggca ggtatgaagc ggggcgtcgg tgcggaaaaa
      541 atgccggtct gggtgccggt atcggaggag ggcacggaaa catgatgcat atcagaaatt
      601 tttcgtatta caccccggcg gagccggatg ttgcaggcgc gatgtacctg aagtctgagg
      661 atggtcagga ctggtatgag tgtcaggcgt tgttttcgcc ggagacgctg aaggtggtgt
      721 atgacagccg gggtgtgatt acgggatacg gtaaggacac tgcgttactg tggccggtga
      781 accagagtgt ggcagaagtg ccggataccc cggagagccg gaaaattgac ctgagcgggc
      841 gctggggttt tgacggggag aaaatcacgg acctgctgac cgcggagaaa gcgcgaggga
      901 tgaagggcga tgaaattaac gcctggcgta atgcgatgga agcggctaac tacacgtttg
      961 agcacaatgg gcgtaaatgg gactacggga agtcaacgca gacgcgtctt gagccgtcgg
     1021 tggcggcagc gaaagcgggg aaactgccgg aggcgttttt ctggacggat gcggaaaaca
     1081 atgatgtgga agtgacagca gaagagctta tagcgctgag tgaagcggca gagcaggcga
     1141 tgttcaccaa agggatggaa atccacgtcc ggcagcgcac catgaagaag gagctggaaa
     1201 agctgaccag tgcggatgag atactggcat acagggttgg ctgggcacag gaataacaga
     1261 caaaaagatg ggggaccatc acctccccca tgaactgagt cgattatcta attgttgtga
     1321 agagacttta cctacacgga tgcagagtgc cacggaaaag ttaatcctga aagtgatacc
     1381 ggtctcattt ccagagcatc ctgttgatat ggataatttt tcagatttcc ggaagtgaga
     1441 gttttggtgt ttccatacca gccacagttg atgtgtttta tggcgcctgg cagtgcgatt
     1501 aagggaacag ggataatgaa aaaacggggg tatcaccacc cccggtacaa ggaagtatta
     1561 actataatgg aaacattaat gaagatcttt agtgctacgt tatatggcga ataaagactg
     1621 ccacag
//
LOCUS       DAZGBC010000397         1623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGBC010000397 DAZGBC010000000
VERSION     DAZGBC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.399"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1002
                     /gene="eilA"
                     /locus_tag="ACHK1J_005284"
     CDS             <1..1002
                     /gene="eilA"
                     /locus_tag="ACHK1J_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFO7914399.1"
                     /translation="NILIEKIPNINFKSINTQQMPSLDSAVMYMNGRMEMYRYTPESL
                     KRALVIFRDCVSTQPQNTLPYCCLAECHISLALLGISEQKQAITTAVTSIEKALEINP
                     SNSQALGLLGLISGLKDEHSVSNVLFKQAHLLKPNSPDVYYYQSLLYFLNGDLARAFN
                     LIEKSIALEPNKMGISILKLIILYYTSPLDNAISFALNLNSQNTCNNPIITSILAMFM
                     ALKGHNDKAKSLLLKLEPEHGLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKIN
                     GVILPLIYSVYGKKEYEKRWQQLIKESDLWSNVLLHDPRLLSVKNEFNTIGVMRSSA"
     gene            complement(1052..>1623)
                     /locus_tag="ACHK1J_005285"
     CDS             complement(1052..>1623)
                     /locus_tag="ACHK1J_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914400.1"
                     /translation="PFVEVESLSVNGAKFRAADGFPKTGFDGAKFTLILTHNMKNTDY
                     NWTSGIQGIQVDSNGMVTLEFIINKEVTITGTPKSNKGNKVTYKFSLQKWFIPQGIIQ
                     ESWSEMNSYCIGNGYILPSSTDLVGSSTSGAVPRKVGSLWGEYGNLTSYDGIFRAEHY
                     WLDSGMIFYPGDGHLSIAPRSSPLCMKTF"
BASE COUNT          534 a          325 c          273 g          491 t
ORIGIN      
        1 aatattctta ttgaaaaaat acccaacatt aattttaaat ccattaatac acaacaaatg
       61 ccatctctgg attctgctgt tatgtatatg aatggcagaa tggaaatgta ccgttataca
      121 ccagaaagtt tgaaacgagc attagtaata tttagagatt gtgtgagtac gcaaccgcaa
      181 aataccttgc cctactgttg cctggcggaa tgccatatat cattagcact ccttggaatc
      241 agtgaacaaa aacaagcaat aactacggca gtaacctcca ttgaaaaggc tctggaaatt
      301 aacccttcaa attctcaggc attgggttta ctgggtttaa tcagtgggct aaaagatgaa
      361 cattcagtgt ctaatgtgct atttaaacaa gcacatttac tcaaacctaa ttcgccagac
      421 gtttattatt atcagtcgct gctatatttt ttgaacggcg accttgcaag agcatttaat
      481 ttaatagaaa aaagcattgc tcttgaaccc aataaaatgg gcataagcat tcttaaatta
      541 attatacttt attatacatc accactcgat aacgcgatat cttttgcatt aaatcttaac
      601 agtcaaaaca cctgtaacaa tccaattatt acatctattc tggctatgtt catggcactg
      661 aaaggacata atgacaaagc caaaagttta ttgcttaaac ttgaacctga acatggtctt
      721 gattacacct gcgttaactc gctttatacg aagtttctga tctatggcgc atctataaaa
      781 aatgacatta tgaagttact ggcaaatatt aacaccaaca aaatcaatgg tgttattttg
      841 ccgttaattt acagtgttta tggtaaaaaa gaatatgaaa agcgctggca acaattaatt
      901 aaagagagtg atttatggtc aaatgtgttg cttcatgacc cccgtttgct cagtgtgaaa
      961 aatgaattta atactattgg tgtgatgcgg agttctgcgt aagagcgaag ataaattgcc
     1021 ctgctgatgc agggcaataa cataaataat atcagaacgt tttcatacac agtggggatg
     1081 aacgtggcgc aatcgagaga tgaccatctc ctggataaaa tatcataccg ctgtcgagcc
     1141 aataatgttc agcgcgaaat attccgtcat acgaagtcag attaccatat tcaccccata
     1201 acgaacctac ttttcgcggg acagcaccgc tagttgaact cccaactaaa tccgtactag
     1261 acggtagtat ataaccatta ccaatacaat atgaattcat ctcactccat gattcctgaa
     1321 ttatcccttg aggtataaac catttctgca gtgagaattt atatgtaact ttattccctt
     1381 tattgctttt cggcgttcct gtaatagtaa cctctttatt gataatgaat tctaaagtca
     1441 ccataccatt actatcgacc tgaataccct gtatgccact ggtccaatta taatctgtgt
     1501 ttttcatatt gtgggttaat atcagtgtaa atttagcacc atcaaaccct gttttgggaa
     1561 aaccatctgc cgccctgaat tttgcaccat tgacactaag cgactcgact tccacaaaag
     1621 gaa
//
LOCUS       DAZGBC010000398         1600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGBC010000398 DAZGBC010000000
VERSION     DAZGBC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.400"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..455
                     /locus_tag="ACHK1J_005286"
     CDS             129..455
                     /locus_tag="ACHK1J_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914401.1"
                     /translation="MQTITTHEQLLVDGKITERTATHIVTGAHGYETLCTSGYNIRYN
                     EKQELVESCEKIAEGELPVTCPTCFAVWQDVHKFTPGDFDTESGKGDFADTNLTEIMI
                     GKMSDT"
     gene            complement(760..1077)
                     /locus_tag="ACHK1J_005287"
     CDS             complement(760..1077)
                     /locus_tag="ACHK1J_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO7914402.1"
                     /translation="MKQFSVYKNKSRNKQAYPYFIDIQAEMLAHLNTRLVMPLTPKDN
                     SNSQVKTLTPVININQVDYVVLTTMITTTDAKNLNSDNVVMDASYLRDQLVSAIDMMI
                     LGI"
     gene            complement(1077..1322)
                     /locus_tag="ACHK1J_005288"
     CDS             complement(1077..1322)
                     /locus_tag="ACHK1J_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO7914403.1"
                     /translation="MRTNVAVKKRSINVYLNAGLLEEAKDLDLNVSAISNKALEKAVR
                     ERKRERWIEENRAGIEALNRFVEDTGIFSDDENFGVI"
BASE COUNT          489 a          367 c          321 g          423 t
ORIGIN      
        1 tttcattttt gtgatgtctg tcatgtgttt ttttgagcaa tgtgaaataa gatgctctcc
       61 cgtcatctga cgtatatttt tacggtcgcc ccaacaatca cataaaccaa caattatgag
      121 agacagacat gcaaacgatc acaacgcatg aacagttatt agttgatgga aaaatcacag
      181 aaagaaccgc cacacatata gtgacgggcg ctcacgggta tgaaacgctt tgcacaagcg
      241 gctataacat caggtacaac gagaaacaag aactggtaga aagttgtgaa aagatagcgg
      301 agggcgaact acctgtaaca tgccctacct gcttcgcagt ctggcaagac gtacataagt
      361 tcacgccagg cgactttgac actgaatcgg ggaaaggaga ttttgctgat acaaatctca
      421 cagaaatcat gattggaaaa atgtcagaca cataaaaaaa acaatcatca cataaaaaat
      481 aatgatacga ctacgaaaca attcaaacaa cattgcagaa ctgctccaaa gtttggaata
      541 tgccgcttta gcggcatgga aggcgcgaaa aatggtttca tagagtcaca gatgccaggt
      601 cgcgccgata cccctaacca gggagtttca tgtcgccagg aaccgcgtca gccccctgct
      661 aagcctgacc gcacggaacc tacagctatt caggtggtag gtggtcgtag accgtcgatg
      721 ttacagcgat attttttact atttactacc ctgcaaaaat taaataccca gaatcatcat
      781 gtcaattgca gaaaccagtt gatctcgtaa atatgaagca tccataacga cattatcaga
      841 gttcagattt tttgcatccg ttgtcgttat catagtagtc agaaccacat aatctacctg
      901 gttgatgttt attactggcg ttaacgtctt tacctgcgag tttgaattat cttttggcgt
      961 taaaggcatc accaaccggg tgttaaggtg tgcaagcatc tctgcctgta tatcaataaa
     1021 ataggggtac gcctgtttat ttcgcgattt gttcttgtag acagaaaatt gtttcactaa
     1081 attaccccaa aattttcatc atctgaaaaa atcccggtat cttccacaaa acggtttaat
     1141 gcctcaatgc cagcacggtt ttcttctatc cagcgttcac gcttacgctc ccttaccgct
     1201 ttttcgagag ctttgttaga gatagcagaa acattcagat caagatcttt tgcttcttca
     1261 agtaaccctg catttaagta tacgtttata ctacgttttt ttactgccac atttgttctc
     1321 ataacaagcc ccccacataa attatgcata taaacatagc atatacccaa aaccccggat
     1381 ctagaagtgt ggaatatgcc gctttagcgg catggaaggc gcgaaaaatg gcttcataga
     1441 gtcacagatg ccaggtcgcg ccgatacccc taaccaggga gtttcatgtc gccaggaacc
     1501 gcgtcagcct cctgctaagc ctgaccgcac ggaacctaca gctattcaga tggtaggtgg
     1561 tcgtagaccg tcgatgttac agcaatattt cacacaaaca
//
LOCUS       DAZGBC010000399         1596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGBC010000399 DAZGBC010000000
VERSION     DAZGBC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.401"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..1124
                     /gene="ompC"
                     /locus_tag="ACHK1J_005289"
     CDS             24..1124
                     /gene="ompC"
                     /locus_tag="ACHK1J_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7914404.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGKDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1288..>1596
                     /locus_tag="ACHK1J_005290"
     CDS             1288..>1596
                     /locus_tag="ACHK1J_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO7914405.1"
                     /translation="MIMTNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKL
                     LNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT
                     D"
BASE COUNT          470 a          326 c          340 g          460 t
ORIGIN      
        1 gataattaaa aggattctgt taaatgaaat taaaaatagt tgcggtggtt gtaactggtt
       61 tgttagctgc gaacgtagca cacgctgccg aagtctataa caaggatggt aataaactcg
      121 acctttatgg caaggttacc gctctacgtt attttactga tgataagcgt gacgatggtg
      181 ataaaactta tgcccgtctc ggctttaaag gagaaacgca aatcaatgat caaatgattg
      241 gttttggtca ctgggaatat gattttaaag gctataacga tgaagccaac ggctcgcgcg
      301 gcaacaagac ccgtctggcc tatgctggtt taaaaattag tgaatttggc tctctggact
      361 atggccgtaa ctacggtgtc ggctatgaca ttggttcatg gactgatatg ttgccagaat
      421 ttggtggcga tacctggagt cagaaagatg tcttcatgac ataccgtacc accggtgtag
      481 caacctatcg caactacgat ttctttggct taattgaagg gctgaacttt gccgcgcaat
      541 atcaaggcaa aaatgaacgt actgacaaca gtcatcttta tggtgctgac tacacgcgtg
      601 ccaacggtga cggtttcggt atctcctcaa cttatgttta tgatggcttt ggtatcggtg
      661 cggtatatac caaatccgat cggacaaatg cgcaggaaag agccgctgct aatcctctca
      721 atgcctccgg taagaatgca gaactgtggg ccacaggtat aaagtatgat gccaacaaca
      781 tctactttgc agctaattac gctgaaacat taaacatgac cacctatggg gatggttata
      841 tctctaacaa agcacaaagt tttgaagtgg tggcgcaata tcaattcgac ttcggcttgc
      901 gcccatcact cgcttacctg aaatcgaaag gcaaagatct gggccgctac ggcgaccagg
      961 acatgattga gtatatcgac gttggtgcga cgtatttctt caacaaaaat atgtcgacct
     1021 atgttgatta taaaatcaac ctgattgatg aaagcgactt tacccgtgcc gtagatattc
     1081 gcaccgataa catcgtcgct acgggcatta cctatcagtt ctaacatcct ctcctgacca
     1141 gcccttctct gggctggttt ttgatttaag tgctgctgta tgactcctta agtaaaaatg
     1201 agcaattttt gatatttctg gtcattcacc tcaattaata ttattccata ccccccgggg
     1261 acatcttact ataatattta ttgatgagtg attatgacca atattaatac agcttgtgta
     1321 aaaaataatg ccagttatca atttaataac gcattaccta acaaagaaac catctccagc
     1381 aatttttgtg aacgactaga acaatggggt aataagttgc ttaataatgg tgaagaaaga
     1441 gcaattgccg tggagcgaat taaagaagct tacaattcga acatggcatc tcttgatttg
     1501 tcatatcttg atttgagtga gttaccgcct atcccctcga cagttaatac gttaaatctg
     1561 gaaaacaatt gtctaacttg tcttgacttt actgac
//
LOCUS       DAZGBC010000400         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGBC010000400 DAZGBC010000000
VERSION     DAZGBC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.402"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            163..834
                     /gene="queE"
                     /locus_tag="ACHK1J_005291"
     CDS             163..834
                     /gene="queE"
                     /locus_tag="ACHK1J_005291"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO7914406.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1173..1567
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1173..1201
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          403 a          350 c          406 g          428 t
ORIGIN      
        1 cttttaatta attccttaaa acataatact ctgcttcatt ttattcttta tgatctaatc
       61 cactttttgc cataaacctg cctgtaacag aatgttaata agacatgatt gttgtggcag
      121 gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta cccgattaac
      181 gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc catttttatt
      241 cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac ctgggaaaag
      301 cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga gagtgataag
      361 tggggggcgg cgagcagtga agacttgctg gctgtcattg gtcgccaggg atataccgcg
      421 cggcatgtgg tgattacggg tggtgagcct tgcattcatg atttgctgcc actgactgac
      481 ctgctcgaaa agaacggttt tagctgccag attgaaacca gtggtactca tgaagtacgc
      541 tgtacaccga atacctgggt taccgtatcg ccaaagctga acatgcgcgg cggctatgaa
      601 gtgttgtcac aggcactgga gcgagccaac gaaatcaagc atccagtggg acgcgtacgt
      661 gatattgaag cactggatga acttttggcg acgctgaccg atgataaacc gcgagtcatt
      721 gcactgcagc cgattagcca aaaggatgat gccacacgtt tgtgcattga aacctgcatt
      781 gcgcgtaatt ggcgtttgtc gatgcaaacc cataaatatt taaatattgc ctgattaaac
      841 atttataagc gttataaatg ggtggagctt gtaaagaatt ccactcattt gtattctttg
      901 tcgtctctga aaaacctcca ttttgcccat cctgaactaa tcattatcat tctctacaaa
      961 ttctgtggcg ttaatttttc gctggagtga aaattattgc ggtaaagttt ggtagatttt
     1021 agtttgtata gagttatttt aaatatttac ctttttaatc aattggttaa gtgctcttta
     1081 acataatgga tgtgttgttt gtgtgatact gtaaagttgg tagattgtga ctggcttaaa
     1141 aaatcattaa ttaataatag gttatgttta gagagttccc cgcgccagcg gggataaacc
     1201 gggcgcttcg gtgtcgatct gggcgttgat ggcgagttcc ccgcgccagc ggggataaac
     1261 cgatgtcggc tgcaatctgc cgcgccgggc gtgtgagttc cccgcgccag cggggataaa
     1321 ccggctggat acagtctggc agggttctgt gatgagagtt ccccgcgcca gcggggataa
     1381 accgatgatg cagccgtctc aggacgacct tcgcacgagt tccccgcgcc agcggggata
     1441 aaccgaacgg ctacggcact gaggtgacaa accagatgag ttccccgcgc cagcggggat
     1501 aaaccggttt ctggcatcgg aaaacattgc atatcgtcga gttccccgcg ccagcgggga
     1561 taaaccggtt tctggcatcg gaaaaca
//
LOCUS       DAZGBC010000401         1575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGBC010000401 DAZGBC010000000
VERSION     DAZGBC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.403"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..149
                     /locus_tag="ACHK1J_005292"
     CDS             <1..149
                     /locus_tag="ACHK1J_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914407.1"
                     /translation="DLINNYSGFILTGRLFWQGEGFGHELFPVTNKYNAWRWKNKKII
                     DISW"
     gene            168..1575
                     /locus_tag="ACHK1J_005293"
                     /pseudo
     CDS             168..1575
                     /locus_tag="ACHK1J_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
BASE COUNT          437 a          347 c          483 g          308 t
ORIGIN      
        1 atgatttaat taataattac agtggattta ttttaacggg gagattgttt tggcaagggg
       61 aaggttttgg ccatgaatta ttccccgtga caaacaaata taatgcttgg agatggaaaa
      121 ataaaaaaat aatagatatt tcttggtaag gaaaatattc cccgaaagag gccacatggg
      181 gaatactgct cccctcactg gtacgggagt accgttacga cagcgcgggt aacgtatcag
      241 gcgtcaccag ccgggaagat tacggacggg aaacacagcg ggagtaccgg cttgaccgga
      301 acggccaggt cacggcggtg acagcctcag gcaccgggct gggctatggc gaaggcgatg
      361 agtcctatgg ctatgacagt tgtggctacc tgaaggcgca gtctgcgggc gggcaccgga
      421 taagtgaaga gactgaccag tatgccggag gccaccggct gaaacaggcc ggaaacacgc
      481 agtatgacta tgacgccgca ggccggatgg tcagccgcac cagacaccgt gacggctatc
      541 gcccggaaac agagcggttc cggtgggaca gccgggacca gctgaccggg tattgcagcg
      601 cacagggtga gcagtgggaa taccgccacg acgccagcgg cagacggacg gaaaaacgct
      661 gtgaccggaa gaaaatccgt tttacgtacc tgtgggacgg cgacagtatt gcggaaatcc
      721 gggaataccg cgatgatgaa ctgtacagcg tacggcacct ggtgtttaac ggctttgagc
      781 tgataagcca gcagtgcagc cgggtacggc agccgcaccc gtccgtggcc ccgcagtggg
      841 tgacacgaac gaatcatgcg gtaagcgacc tgacgggccg cccgctgatg ctctttaaca
      901 gtgaaggcaa gacggtctgg cgaccgggac agaccagcct gtgggggctg gcactcagcc
      961 tgcccgcaga cacagactac ccggacccgc gcggggagct ggacgcggaa gccgaccccg
     1021 gcctgctgta tgcaggacag tggcaggatg cagaatcggg gttgtgctat aaccggttcc
     1081 gatattacga gccggaaacc gggatgtacc tggtgagtga tccactgggg ttgctggggg
     1141 gtaagcagac ttaccggtat gtgccgaatc cactggggta tattgatccg ctggggctgg
     1201 taaaaacttc tgttcccgca gaaaaaataa gcctttcgga taaagcaaga gatcttttta
     1261 gacaaggaaa agtaagagaa gcattagatg ttcattatga ggatcttgta cgaagaaagc
     1321 ttggtggtat atctcaagaa attgccgggc gtgaatatga tgttgttact gacaagatca
     1381 tagcgcaggt caagaggact tatagctcta tagataatcc aaaaaacttc ttaagtaagt
     1441 ctactcggac acaaattaag aagacaattg agctggctga agaacagggg aaagaggctc
     1501 agttttggtt taaatatgga gtaagcccta aagtaagaga atatatagaa agcaaaggtg
     1561 ggaaagttat attgg
//
LOCUS       DAZGBC010000402         1573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGBC010000402 DAZGBC010000000
VERSION     DAZGBC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.404"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..268)
                     /locus_tag="ACHK1J_005294"
                     /pseudo
     CDS             complement(1..268)
                     /locus_tag="ACHK1J_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
     gene            325..1538
                     /locus_tag="ACHK1J_005295"
     CDS             join(325..616,616..1538)
                     /locus_tag="ACHK1J_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7914408.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSPS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    606..722
                     /locus_tag="ACHK1J_005295"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          426 a          372 c          435 g          340 t
ORIGIN      
        1 ccatacgttt tggccatgca tttactgaca atacgataaa gaacaacaga aagataaatc
       61 cccggtggct tttatcacgc ttacgccaga accagaatgt aaaagcagca atgaacagtg
      121 aaacaataaa ataaagaaca ggtatgcctg tgataaattc acctgcctca tttaggtttg
      181 tctgcattac agacgcaata aagctgttgt ttatttttcc gtaaatcatc ccaaccggag
      241 catagaatgc actaaacaaa agaaagatac tgaaccgccc cgggaatcct ggagactaaa
      301 cttcctgaga aagaggtaaa caggatgact aaaaatactc gtttttcccc tgaagtccgt
      361 caacgggcag ttcgtatggt tctggaaagt cagggcgaat atgactcaca atgggcggca
      421 atttgttcca ttgctccaaa gattggctgt acgccggaga ctctgcgtgt ctgggttcgc
      481 cagcatgagc gggataccgg gggcggtgat ggagggctca ccaccgctga acgtcagcgt
      541 ctgaaagagc tggagcgtga aaatcgtgaa ctgcgccgca gtaacgatat ccttcgccag
      601 gcttccgctt attttgcgaa ggcggagttc gaccgcctct ggaaaaaatg atgccactgc
      661 tggataagct gcgtgagcag tacggggtcg gaccgctatg cagcgaactg catattgccc
      721 cgtcaacgta ttaccactgt cagcaacagc gacatcatcc ggataaacgc agtgcccgtg
      781 cgcagcgcga tgactggctg aagaaagaga tacagcgcgt atacgatgaa aatcacaagg
      841 tatacggtgt gcgtaaagtc tggcgtcagt tgttacggga aggtatcaga gtggccagat
      901 gcactgtggc acgtctcatg gcggttatgg gacttgccgg tgttctccgg ggtaaaaagg
      961 tccgtacgac catcagccgg aaagccgttg ccgcaggcga ccgcgtaaac cgtcagttcg
     1021 tggcagaacg acctgaccag ctgtgggtgg ctgattttac ttacgtcagc acatggcagg
     1081 gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg atacattgtg gggtggcggg
     1141 tctcaccgtc catggagacg acattcgtgc tggatgcact ggagcaggcg ttatgggccc
     1201 gtcgaccgtc cggcacggtc catcacagtg ataaaggttc tcagtatgta tcgctggcct
     1261 acacacagcg gcttaaggaa gccggattac tggcatcaac aggaagtaca ggcgactcgt
     1321 atgacaacgc gatggcggag agcatcaatg gtctttacaa agcggaggta atacaccgta
     1381 agagctggaa aaaccgtgca gaagtggaac tggccacact cacgtgggtg gactggtata
     1441 acaatcgacg attgctggaa aggctgggcc atactcctcc ggcagaagca gaaaaagctt
     1501 attatgcttc catcggaaac gatgatctgg cagcctgagt tcacagataa aacactctcc
     1561 aggaaacccg ggg
//
LOCUS       DAZGBC010000403         1566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGBC010000403 DAZGBC010000000
VERSION     DAZGBC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.405"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..674
                     /locus_tag="ACHK1J_005296"
     CDS             156..674
                     /locus_tag="ACHK1J_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7914409.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            884..>1566
                     /gene="tssI"
                     /locus_tag="ACHK1J_005297"
     CDS             884..>1566
                     /gene="tssI"
                     /locus_tag="ACHK1J_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO7914410.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVT"
BASE COUNT          367 a          417 c          412 g          370 t
ORIGIN      
        1 atagttttta cgacaaccgt aaattctagc tcggtttgta tgtaaaaaat actgaaaaat
       61 gatgcggttc cgtgaagtga gctgcatctg cctgtctggg attaacggca ggtcattgta
      121 agtagtaatt aaccacgtta acaaggagtt tacaaatgcc aacaccgtgt tatatctcca
      181 tcactggtca gacccaggga aatattactg caggtgcatt taccgctgat tctgtcggca
      241 acatctatgt gcaggggcac gaagacgaga tgctggtgca ggaattcctg cataacgtta
      301 ctgtcccgac cgatcctcag tccggtcagc ctgcgggtca gcgtgctcac aagcctttca
      361 tttttaccgt ggcgctgaac aaagcggttc cgctgatgta caacgcactg gcctccggcg
      421 aaatgctgcc aaccactgaa cttcactggt ggcgtacctc tgtggaaggc aaacaggagc
      481 attatttcac tacccgcctg actgattcga cgattgtcga tatgaaactg catatgccgc
      541 attgccagga cccggcgaaa cgcgagttca cccagttgct cgaagtttca ctggcctacc
      601 gcaaaattga atgggaacac gtcaaatctg gcacctccgg tgccgacgac tggcgcgcac
      661 cgctggaagc ataagttaag ccaacagcat ccggctatca ggccggatgc tgtttttgta
      721 ttacgccgta gaaagaaagg ttgtcagtaa ggggttacgt tcacgggaga tggcggctgc
      781 cggaaacttc ggcggtagcg tgcctgaacg caagcgtaac actgtactgt cacagccgct
      841 ttaacagcga actgtctgtt tttccttaac cccggaggtt gctatgtcaa ccggattacg
      901 tttcacgctg gaagtggacg gcctgccacc ggatgctttt gcggtggtct cctttcatct
      961 gaaccagtca ctctcttcgc ttttttccct cgctctctct ctggtcagcc agcagtttct
     1021 ctcccttgaa ttccagcaga tcctcgacaa aatggcctac ctgacgatat ggcagggcga
     1081 tgacgtacag cgccgggtga aaggtgtggt gacctggttt gaactggggg agaacgacaa
     1141 aaaccagaag ctgtacagca tgaaggtatg cccgccgctg tggcgcacag ggctgcgcca
     1201 gaacttccgt atcttccaga atgaggacat cgaaagcatc ctcggcacga tattgcagga
     1261 aaacggggtg accgagtgga gcccgctgtt cagcgagcca catccttccc gtgagttttg
     1321 tgtccagtac ggtgagactg attacgattt cctgtgccgg atggcggcgg aggaaggcat
     1381 cttcttttat gaggagcacg cgcaaaaaag taccgaccag agcctggtcc tgtgcgatac
     1441 cgtgcgttat ctgccggagt cctttgagat cccctggaat ccgaacaccc gtaccgaggt
     1501 gagcaccctc tgcatcagcc agtttcgcta cagcgcacaa atccgccctt cttccgtggt
     1561 gaccaa
//
LOCUS       DAZGBC010000404         1554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGBC010000404 DAZGBC010000000
VERSION     DAZGBC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.406"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1374
                     /locus_tag="ACHK1J_005298"
     CDS             <1..1374
                     /locus_tag="ACHK1J_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO7914411.1"
                     /translation="SGSGNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGL
                     VKTPVYGVPEAGGNSDVQGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLND
                     NGRAIGILRTGVRPYVGSEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQA
                     EDAWDRLPPNVRKFDVDVEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKV
                     DHKTPEEIYEALGFNNEEPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDM
                     GNKIGDYLGESYKSLAREIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADHD
                     ALVNAWRSRDAQDIANKLGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVE
                     SWVLSGMAASVAMTLLSAIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINN
                     KLVHFTH"
     gene            complement(1397..>1554)
                     /gene="cui"
                     /locus_tag="ACHK1J_005299"
     CDS             complement(1397..>1554)
                     /gene="cui"
                     /locus_tag="ACHK1J_005299"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="HFO7914412.1"
                     /translation="ELTSSAKMLKLMSVNIILLSFFYISLHSIIFIFSYLYFWFCIGS
                     YRSFKEK"
BASE COUNT          514 a          228 c          383 g          429 t
ORIGIN      
        1 tcaggctctg gtaatggagg tggtgaacat ggaagtcaga cggggtgggg atggagtaaa
       61 accaataatc cggatgtgcc gccttatgtt gatgataatg ggcaggtgcg cattaccatt
      121 acaaatggtc tggtaaaaac gcctgtatat ggagttcctg aggctggcgg aaatagtgat
      181 gttcaggggg gctatattcc tgaaaatcct aatgatgaag ttgctcgtaa gtgggataaa
      241 aacaacttac cgcgagagat tgatgtgagt atcgatggtt ttaaatatcg tgtaacgctt
      301 aatgataatg gtagagctat cggtattctt cgaacaggtg tccgtcctta cgtagggagt
      361 gaaaaagcta aggcgggtat tatggaaaaa atcaatcata aaacacctga ggagatatat
      421 gaggcgttag gttttaataa agatgaatct cagcggcagg aaaaagcaaa acaacaggct
      481 gaagatgcgt gggatcgttt gcctcctaat gtgagaaaat ttgatgttga tgttgaacag
      541 ttccactatt tagtggtgct tgatgattat ggtaatgttt tatctgtaac tagaacagct
      601 gttcgtcctt atgtagggag tgaaaaagct aaggctggta ttatggataa agtcgaccat
      661 aagaccccgg aagagatata cgaggcatta ggatttaata atgaagagcc tcaacgacag
      721 aatcaagcta aaaaagctgc ttatgatgta ttttattctt tcagtatgaa ccgtgaccgg
      781 attcagagtg atgtgctaaa taaagcagct gaacttatat ctgatatggg taacaagatc
      841 ggtgattatc ttggtgaatc ttataagtcg ttagcaagag aaattgctga tgatatcaaa
      901 aatttccagg ggaaaaccat ccgtagctac gatgatgcaa tggagtcatt aaataaaatt
      961 ttgtctaatc ctagttttaa agttaacaga gcagaccatg atgcactggt gaacgcctgg
     1021 cgaagtcgtg atgctcagga tatagcaaat aaactgggga atatcagtaa ggctttcaaa
     1081 gctgccgatg taattatgaa gattgagaag gtcaaggaga aaagcatcga gggatatgaa
     1141 actggaaact gggggccgct gatgttggag gttgagtctt gggttctcag tggtatggcc
     1201 gcaagcgttg ccatgacatt gcttagtgct ataattggat cattcacact accggcgtta
     1261 gcattaactg caataaatat tgcaggtatt atgggaatta gttaccttgc atcatatatt
     1321 gatgcaagtg tggctgaaaa aataaataat aaattggttc attttactca ttgatagaaa
     1381 aagagaggtg atcatatcac ttctctttaa acgacctata ggaaccaata caaaaccaaa
     1441 aatacaaata tgagaatata aaaataatgg aatgtaagct aatgtaaaaa aacgatagga
     1501 ggattatatt tacagacatc agttttagca tttttgcaga actggttagc tccg
//
LOCUS       DAZGBC010000405         1549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGBC010000405 DAZGBC010000000
VERSION     DAZGBC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.407"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1549
                     /locus_tag="ACHK1J_005300"
     CDS             <1..>1549
                     /locus_tag="ACHK1J_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO7914413.1"
                     /translation="NGIIDLKDNVNQGAGYLQFADDYTVTTSNASSWFGGGIIVNSGT
                     TVKWGINGVSGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNNDSTAFSSI
                     NISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDINGKNTEFSRLQAVDYGAAIINSSTE
                     KSLLTLNLSPLKKDEIAVSVKALDMNAIFHGGYGTTGDLYKTTFYGPTQYYLLKKPKF
                     GSVLMGSLKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNMDIVIPEL
                     TGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNL
                     NNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSS
                     YTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGAL
                     TAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQ
                     ASVVGDII"
BASE COUNT          460 a          293 c          377 g          419 t
ORIGIN      
        1 aaatggcatt attgatttaa aagataatgt taatcaggga gccggatatc ttcagtttgc
       61 ggatgattat acagtaacaa cgagcaatgc cagttcatgg ttcggaggag gcattattgt
      121 caattcgggt acaactgtaa aatggggtat aaacggggtt tccggagatg accttcataa
      181 agttggggac ggaacgctta ttattaatgg cacagggaaa aatgaagggg gactgaagat
      241 tggggctggt actgtaatcc ttgaacaaaa agcgaaaaac aacgattcaa cggctttttc
      301 ttccattaac atcagtgggg gaaactcgcg cgttaaactc tcaggagata atcagattat
      361 acctgataat gtttcctggg gattccgggg tggatattta gatataaacg gaaaaaacac
      421 agagttctcc cgcttacagg ctgttgatta tggggctgca attattaaca gcagcacaga
      481 gaagtcatta ttgacgttaa atctgtcacc attgaaaaaa gatgaaatag ctgtgagcgt
      541 gaaggcgtta gatatgaacg ccatctttca tggaggatac gggacaaccg gcgatttgta
      601 taaaacaact ttttacggtc cgacacagta ttatctgctg aaaaagccca aatttggcag
      661 tgtgctgatg gggtcactga aaaacaccag cgaatggcaa tttgcaggaa cagacctgaa
      721 tcaggccgtt gatatggcaa agaataataa actgacatca agtgcacagg cttcatatct
      781 ttatcacggt aagctcctgg ggaatatgga tattgtcatt ccggagctta caggcaatga
      841 tattttaacg cttgatggct ctgtcagtat atcaggagat atgtcaaaac aggacggtgc
      901 tcttatcttc cagggacacc cggttattca tgcagggcaa actgtttctg catcgcagag
      961 tgactgggag aacagggagt tctcactcaa caatctgaat cttaataatg cggacttcag
     1021 tctgtcccgt aatgcattta tgaacgggaa tatcagggcc gttaaccaga gcactgttat
     1081 tatcggcgga gatacagtct ttactgataa aaatgacgga acaggtaatg atgtcatcag
     1141 tgttgaaggg aaatctgctg ccgcaggaac atcctcctat acagggcata tcactctgga
     1201 gcaaaaatca gcactggata tccgcgataa ttttcgtggc ggggttacgt ctgaagacag
     1261 tcatatcaat gtttcttcat cttcagtcct gttctcagat gcatcgtcat ttataaacag
     1321 ctccctgaat attcataaag gaggtgcgct gaccgctcag ggagggctgt ttacaagtgg
     1381 aagcattgat attggtgacg cttcccttct gcttaccggt acaccagtga attcagatga
     1441 tgctgctttt ttaccgacca tcaatatggc tgatggcgga tttaaactga tgtctgattc
     1501 atcagtactg aaagccagag accaggcatc tgttgttggt gatattatt
//
LOCUS       DAZGBC010000406         1539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGBC010000406 DAZGBC010000000
VERSION     DAZGBC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.408"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..283
                     /locus_tag="ACHK1J_005301"
     CDS             <1..283
                     /locus_tag="ACHK1J_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914414.1"
                     /translation="IKFPETAEELLNNALVTLPADANYKDKTLLLKAIYSGCTNLPFS
                     LFINNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            276..>1539
                     /locus_tag="ACHK1J_005302"
     CDS             276..>1539
                     /locus_tag="ACHK1J_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO7914415.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRAS"
BASE COUNT          441 a          249 c          329 g          520 t
ORIGIN      
        1 aataaaattt cctgagactg cagaggaact cctgaacaat gcactggtga cccttcccgc
       61 agatgctaat tataaagaca aaacgttact cctgaaggcg atttacagtg gatgtacaaa
      121 tcttccattt tcacttttta ttaataatga acaattatta gagattaggg agtgttgtaa
      181 acaggcaata aaagtaacat ttgcagcaga gctctttgat acacaaaact gtaacaaaaa
      241 acaaaataaa aaaccatgga agaaggtgat gttcaatgtt taacaaagtt ttggtagggt
      301 taaggagtca tccagaatta ataattcttg gattaatggt gatgattatc gccatgctca
      361 taattccatt gcccacctat ttaatcgact ttttgattgg gcttaactta acattggcaa
      421 tacttgtatt tttaggatca ttctatgttg ataggatctt aagcttttct tcatttccat
      481 caatcctcct tatcacaacg ttatttcgtt tggcgctggc gatcagtacc agtcgactta
      541 tacttcttga ggcggatgct ggagaaatta taacgagttt tggtgagttc gttattggag
      601 atagcctggt agttggtttt gttattttct ccattgttac tattgttcag tttatcgtga
      661 ttactaaagg ttcggaacgt gtggctgaag ttgctgcccg cttttcgctt gacggtatgc
      721 caggtaaaca aatgagcatt gatgccgatc tacgcgctgg gatcattgat gcagatttag
      781 caaaagaacg tcgtagcgta ttagaacgag agagtcagct gtatggttcc tttgatggtg
      841 cgatgaaatt tatcaaaggt gatgctattg caaacattat tattatcttt gttaatatta
      901 tcggtgggct atcagtagga gtaggccaaa atggaatgga tttctcaaca gcgttgactg
      961 tctacacaat tcttactgtc ggtgatggtc ttgtttctca aattccagct ttattaattg
     1021 ctattagcgc aggatttatc gttacgcgcg taaatggcga tagtgataat atggggcaaa
     1081 atataatgtc ccagttatta agtaattctt ttgtcattat tgtgacatgt gtcctcgcgc
     1141 taagtattgg gttacttcca ggttttcccc ttcttgtgtt tttatgtttg gcagtgatct
     1201 taggaattta cttttacttt aaattcaaaa agaaagggac tgaagaaacg gttgttgagg
     1261 gagatattac cgttgggttg gaaccctata atcaagatga tgacatctca ttaggtatca
     1321 ttaataaact tgatcaggtt attaccgaaa ctgtaccctt ggtcttgatt atgaatagtg
     1381 tacaggctaa aaaatacact gaaatcaatc ttgctgatag gattcgtagt cagtttttta
     1441 ttgagtatgg tatccgcatt cccggtattg tcattcgtga gggggaaggt cttaatgatg
     1501 aagatgtcat attaatgcta aatgaagtca gagcttcgc
//
LOCUS       DAZGBC010000407         1536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGBC010000407 DAZGBC010000000
VERSION     DAZGBC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.409"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..114
                     /locus_tag="ACHK1J_005303"
     CDS             <1..114
                     /locus_tag="ACHK1J_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFO7914416.1"
                     /translation="SAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            453..932
                     /locus_tag="ACHK1J_005304"
     CDS             453..932
                     /locus_tag="ACHK1J_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7914417.1"
                     /translation="MKIVSQNTTTLSSATAPGNNQPQLVATPVPDEQRISFWQQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGKTWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRIID"
     gene            948..1424
                     /locus_tag="ACHK1J_005305"
     CDS             948..1424
                     /locus_tag="ACHK1J_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7914418.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT          354 a          445 c          389 g          348 t
ORIGIN      
        1 agtgcaaaag gaaaacgtat ccacacccgt gccattcaca gcatcgacac cgatattaag
       61 ctcaaccgcg cattgtgggt aatggcagaa acgctgctgg agagcatgcg ctgataaaac
      121 atatccctga aggcgtgatt caggtaccag aatcacccga cgccccggag gcgctgccgg
      181 ggtgtcgttc catttctgat gatgacctgt gcacctggtg tacccgtctg ctttaccgtc
      241 ccggagaact cagtctgtgc agactgagta caggcaatgg catctggcca tccgtctgtg
      301 accggaacgg ttacgcgtac ggctgtccgg aatttcagcc gaatatcatt catccctgat
      361 attaccgggt ctcacggtca gtgatgaacg tgatgttgcc agcccccggc ctttatttcc
      421 tctcatttat acacattgta aggatttcac tcatgaaaat cgtttctcag aacacaacta
      481 ctctttcttc agccacagca ccagggaata accagcccca actggttgct actcccgtcc
      541 ctgatgaaca acgtatcagc ttctggcagc agcattttgg cctcattcca cagtgggtga
      601 ccctggagcc ccgtgtcttc ggctggatgg accgtctgtg cgaagactac tgcggtggta
      661 tctggaatct gtacaccctg aacaacggtg gcgcatttat ggcacccgaa ccggatgaag
      721 atgatggaaa aacctgggta ctgttcaatg ccatgaacgg taaccgcgct gaaatgagtc
      781 cggaagctgc cggcattgct gcctgtctga tgacgtacag tcatcacgcc tgtcgtacgg
      841 agtgttatgc catgacggtc cactattacc ggctgcggga ttacgccctg caacatccgg
      901 aatgcaacgc cattatgcgc atcattgact gaatggaggc agggacaatg cagcagcttt
      961 cctttctgcc cggagagatg acgcccggcg agcgcagtct cattctgcgg gccctgaaaa
     1021 ccctggaccg ccatcttcat gaacccggcg tggccttcac ctccacccgt gcggcacggg
     1081 aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg ctgtatctga
     1141 ataaccagaa tcagctgatt gccggtgaaa ccctcttcac cggcaccatc aaccgcacgg
     1201 aagtccatcc ccgggaggtg attaaacgcg ccctgtacca caatgccgca gccgtggtgc
     1261 tggcacacaa tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgc cttatcacgg
     1321 aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat ctgatagtcg
     1381 gtggcagcca ggttttctcc tttgcggaac acggtctgct ttaacccgtc accgtcacaa
     1441 ccacatcaca cctgttttca cttttatctt ctgtcttcag aggtatcaca ctatgaaaat
     1501 catcacccgt ggtgaagcca tgcgtattca ccaaca
//
LOCUS       DAZGBC010000408         1518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGBC010000408 DAZGBC010000000
VERSION     DAZGBC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.410"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..562
                     /locus_tag="ACHK1J_005306"
     CDS             227..562
                     /locus_tag="ACHK1J_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO7914419.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            1020..1463
                     /gene="yjdN"
                     /locus_tag="ACHK1J_005307"
     CDS             1020..1463
                     /gene="yjdN"
                     /locus_tag="ACHK1J_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO7914420.1"
                     /translation="MPLSPYISFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
BASE COUNT          399 a          411 c          357 g          351 t
ORIGIN      
        1 catgtgaata aaaaaaagaa caagtagagc attaacctta tcttaaataa taaatggagg
       61 caaaaagatt attttctttt tgcgtttcct ttcaaatgaa aacgatcgtc gtctaaaatc
      121 agcagtaccc ccgacaaact cagggatttt gtgtataatt gcggcctttt tcggcaatct
      181 gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa atccacatgt cattaccaca
      241 ctgcccaaaa tgcaactccg aatacaccta cgaagataac ggcatgtaca tctgcccgga
      301 atgtgcctac gaatggaacg acgcagaacc tgcacaggaa agcgacgagc tgatcgttaa
      361 agatgctaac ggcaatctgc tggctgacgg cgacagcgtt accatcatta aagatctgaa
      421 ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa gtaaaaaaca tccgcctggt
      481 tgaaggcgat cataacatcg attgcaaaat cgacggtttt ggtccgatga aactgaaatc
      541 tgagtttgtg aaaaagaact gattgtattg tgatcggtaa gccggataag gcgctcgcgc
      601 cgcatccggc aacggtgcca gatgcctgat gcgacgcttg cgcgtcttat caggcctaca
      661 aattcccgca ccatccgtag gccggataag gcgtttacgc cgcatccggc aacagtgcca
      721 gatgcctgat gcgacgcttg cgcgtcttat caggcctaca aattcccgca ccatccgtag
      781 gccggataag gcgtttacgc cgcatccggc aacggtgccg actgcctgat gcgacgcttg
      841 cgcgtcttat caggcctaca aattcccgca ccctccgtag gccggataag gcgtttacgc
      901 cgcatccggc aacagtgccg actgcctgat gcgacgctcg cgcgtcttat caggccgcct
      961 ctcatctgta taaatttcga actacactta actggcttct cttcactgag gtcaccatca
     1021 tgccgttaag tccctacatc tcttttgccg gtaactgttc cgacgcgatt gcctattatc
     1081 aacgcacgtt gggcgcggaa ctgctctata aaatcagctt cggcgaaatg ccaaaatcag
     1141 cgcaggacag cgccgagaac tgcccttccg gaatgcaatt tcccgatacc gccatcgctc
     1201 atgccaacgt gcgcattgcc ggaagcgaca tcatgatgag cgatgccatg ccgtcaggaa
     1261 aagccagcta ctccggcttt acgctggtgc tcgattcgca acaggtcgaa gaaggaaaac
     1321 gctggtttga taaccttgcc gctaacggaa aaatcgaaat ggcctggcag gaaactttct
     1381 gggcgcatgg cttcggcaaa gtcaccgata aatttggcgt accgtggatg attaatgtcg
     1441 tcaaacaaca accaacgcaa taatccgccg ggaggcccgc cctcccgcac tgtcatcgaa
     1501 ttcccgttaa ctcttcat
//
LOCUS       DAZGBC010000409         1509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGBC010000409 DAZGBC010000000
VERSION     DAZGBC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.411"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1509
                     /locus_tag="ACHK1J_005308"
     CDS             <1..>1509
                     /locus_tag="ACHK1J_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7914421.1"
                     /translation="GEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGY
                     FIEHKSRNSLLFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRF
                     SSGVGFWCLGGAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPK
                     SIRGSTPKIKGSCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDS
                     PESVFYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITF
                     HIWTAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKV
                     VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLR
                     VDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKP
                     VITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPE"
BASE COUNT          359 a          363 c          436 g          351 t
ORIGIN      
        1 ggagaatgga aaacgctgcc gttccagatc gccatcatga acagtatggg gaatgatcag
       61 atccgcactg ttaatctgat taaatctgcc cgtgttggct atacaaagat gttgctgggg
      121 gtggtcgggt attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat
      181 tctgccgctg aagattttat gaagtctcac gtggaggcga cgattcgcga tgttccctgc
      241 ctgaaaaaac tttctccctg gctgggacgt aaacatcgtg ataataccct cacgctgaaa
      301 cgcttttcat cgggtgtggg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt
      361 gaaaaatccg tggatgtggt ctgctatgac gaactttcct cgttcgagcc ggatgttgaa
      421 aaagagggct cgccaaccct gctgggggat aagcgtattg agggctctgt atggccaaaa
      481 tccattcgcg gctcgacgcc taaaataaaa ggttcctgcc agatcgaaaa agcggccaac
      541 gagtcggcgc atttcatgcg tttttatgtg ccctgcccgc actgtgggga ggcgcagtat
      601 ctgaaatttg gcgatgagtc cacgcctttt ggccttaaat gggagaagga cagccccgaa
      661 agcgttttct acctctgtga acatcatggc tgcgtgatcc atcagtctga gcttgaccag
      721 agcaacgggc ggtggatctg tgaaaacacg gggatgtgga cccgtgacgg tctgacgttt
      781 ttcagcgctg cgggtaatga aattccaccg ccgcgctcca tcacattcca tatctggacg
      841 gcgtacagtc cgttcaccac ctgggtacag atagtctatg actggctgga tgcactgaaa
      901 gatcccaacg gcctgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
      961 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcgtta cacggcggcg
     1021 gtgcctgccc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1081 atgtatgtct ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt
     1141 atgggacgtc cagatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa
     1201 tatcgccatg cggatggcac cgaaatgacc atttcccgtg tctgctggga caccgggggg
     1261 atcgatggcg aaatcgttta tcagagatca aaaaaacacg gtgttttccg tgtgctgccg
     1321 gtaaaaggcg cgtctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag
     1381 cggggcgtgt atctgtgtga agtggggacg gacaccgcaa aagaaattct ctatgcccgt
     1441 atgaaagccg atcccacgcc tgcggatgaa gccacgtcgt atgccatccg ttttcctgat
     1501 gatccggag
//
LOCUS       DAZGBC010000410         1506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGBC010000410 DAZGBC010000000
VERSION     DAZGBC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.412"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(20..247)
                     /gene="mchB"
                     /locus_tag="ACHK1J_005309"
     CDS             complement(20..247)
                     /gene="mchB"
                     /locus_tag="ACHK1J_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO7914422.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(275..484)
                     /gene="mchI"
                     /locus_tag="ACHK1J_005310"
     CDS             complement(275..484)
                     /gene="mchI"
                     /locus_tag="ACHK1J_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO7914423.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            593..829
                     /locus_tag="ACHK1J_005311"
     CDS             593..829
                     /locus_tag="ACHK1J_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914424.1"
                     /translation="MIKDNSKVDDSADRLSFTETLFVAHSILPDLTQTKTKENDCYLL
                     FKKKCHIVHIKSRGILRYVTDAIVSQDLMVVAHV"
     gene            1298..>1506
                     /locus_tag="ACHK1J_005312"
     CDS             1298..>1506
                     /locus_tag="ACHK1J_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914425.1"
                     /translation="MYLTKKIIISMMFILPSAAFSSDPPPLQQSLEKTTYFSIGMNGF
                     IGYQSEGEKLYTHILTLDNPEEIFK"
BASE COUNT          527 a          304 c          265 g          410 t
ORIGIN      
        1 aaaataaata aaaatataat tagctaccgc caccagcaga agaactggca ctaccacttc
       61 ccacggtcga gccaattgct gttgtcaaac cggcagatac ggctccaact acaaccccaa
      121 gtggaccacc aggtattccg gcgagagctc caacaatagc tgcagcacct gcagcattag
      181 ctgaagtagc tggcgcacct cccgccccgg aaatatatct taactgtgat tctgttattt
      241 ctcgcatata aactgcatat aaactccatc atatttaact tccgtttttc cagcctgacc
      301 agaccagaat ccttcgaatc ccatgaatca gctcaataaa accaagaaat attataaagc
      361 ttagaaaaac gtcaacataa gaattccctt cgccaacaat ccgaagggat gaaagtgaaa
      421 ccaataagat agtaataggt aaactgaata tagccgtcaa ttgggacagt tttttataac
      481 tcattaatcc ctacctgttg ctttattttc attatgaaaa gttaccaaat cttagcacct
      541 cagtcaggag agtcaggtca tcgataaaga atattccagt agagtggcgc tcatgatcaa
      601 agataacagc aaagttgatg acagcgctga ccgactgtca tttacagaaa ccctgtttgt
      661 agcacattcc atcttacctg acctcaccca gacaaagaca aaagagaatg attgttattt
      721 acttttcaaa aaaaagtgtc atattgtcca tataaagtca agaggtattt tacgttatgt
      781 cactgatgca attgttagtc aggatctcat ggttgtcgct catgtataac ctgagacttg
      841 aacgcacact aatagcactg acggtcatac atcgggaaat gtgaggaggc ataagaatga
      901 attgtgataa taatcacaga aatgaagaat tcattgttac ctttgataaa ggcaacaagc
      961 aagacaattc aagacgaaaa cacgataatt ttcctataga ggtagaatcc tccgtagagc
     1021 aggagacaca ctgtatcaca aataataagt cggcttccgg tatagtaaca catgactatg
     1081 atgccgatta tatttgtggt tgtggtgaaa ttatgtgtcc tggttgcggt catgacctat
     1141 aacaaacatt tttgcaaact gatacctgat caggtacaag cctgttgatt attatgctga
     1201 atatttcatg ttataaaacc atcgatataa acattaacaa gaaaaaacat atattgttta
     1261 tctgaacatt tatcgatgga aaaccaaagg ggaaagtatg tatcttacga aaaagattat
     1321 aataagtatg atgtttatat taccatctgc tgcattttca tcagatccac ctccccttca
     1381 acaatcgtta gaaaaaacaa cctatttttc tataggtatg aatgggttta taggctatca
     1441 gagcgaaggg gaaaaattat acacacacat tcttacatta gataatcccg aagagatatt
     1501 taaaaa
//
LOCUS       DAZGBC010000411         1503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGBC010000411 DAZGBC010000000
VERSION     DAZGBC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.413"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..927)
                     /gene="pilV"
                     /locus_tag="ACHK1J_005313"
     CDS             complement(<1..927)
                     /gene="pilV"
                     /locus_tag="ACHK1J_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO7914426.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQENDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKC"
     gene            complement(932..>1503)
                     /locus_tag="ACHK1J_005314"
     CDS             complement(932..>1503)
                     /locus_tag="ACHK1J_005314"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO7914427.1"
                     /translation="AGHYLMQVHTLRLPRHLSLLCVVLALTATITHIIRGTDITGLCF
                     LLLMAGCMTLMTLTDATSCRLPRPFTLTFLMLGVAFRLWQGDAAIALACALFWFLLLS
                     LLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTPWIIGIAATGTMVCMSLFPRSNT
                     GAAIPFGPFICASLYIFTFFPGAIFHTGG"
BASE COUNT          366 a          397 c          382 g          358 t
ORIGIN      
        1 acactttgtg cctgccgtag ctgttttatc aagctgtaaa tactccccgg ttgaaagacg
       61 accgtcagac cggactgtac caccactgac tttcccccct ttgatttccc cggtggttgt
      121 gatgcctttg ccattaaccg cgcgtatcca ctggctgtcc gtcatggtga atccaccacc
      181 atgtgtgata ttcatccatc ccttattgtt tctggtgatt agccagccgt cttcactcgt
      241 aatatcacct ttcagtgtgg cactctgacc attgatattg ccggtgttgt ttatgttatt
      301 cccgcccata tttattgcag tatgcatctg attcagttca ggacgacctg ctacctgaaa
      361 acggtagaga cggtcatttt cctgcaggtc atcaccgctc atgacgacgg cgatgtgacc
      421 tgtcgaacag gtaagtccgt agtctcccgg tttatctgtc caggctccga atgcgcctgt
      481 tgccgttccg tttttgctga taaatccacc cagccccggc aactgacccg ccacactacg
      541 cagcccttct tctttgattg tctgccctcc agttgagcag gtcagggctt ccagacgccc
      601 ggtggtctgg ttacggcgga taccggtgat ataactctga ccaaacggag aatggttaaa
      661 cccctgtttc agatatccct gacgaatcag atcatcagcc gtgaaatttg ccggaaggct
      721 gccgctatgc agacgggcat tgttgtcggc aatatagttt ctggctgcgg tattgtaggc
      781 atctgcatga tatgcagcac tctggtaaag ctgctcattc agccagtttt caatttctga
      841 ataaatcatt ggtgtggcga taccgatcac aatcagtccg gccagaattt caaacagaac
      901 aaaaccttta tcgtcgtctt tttgcatggt gttaccctcc ggtgtgaaaa atggcaccag
      961 gaaaaaaagt gaaaatgtac agtgatgcac agataaacgg accgaacggg atagcggccc
     1021 ctgtattact gcgcgggaac agcgacatac agaccatggt gccggtggcg gcaataccga
     1081 tgatccacgg agtgctgttg gcagatgacc acatagccac acctgcaatc agaaacacgt
     1141 cccccagtcc cagcgtctcc actcccttgc ggtatgaggt gtagcgcctc aggagagata
     1201 acaggagaaa ccagaacaga gcacaggcaa gcgcaatagc agcatcgccc tgccataaac
     1261 gaaacgccac acccagcatc aggaatgtga gggtgaaggg acggggaaga cggcaggagg
     1321 ttgcgtcagt tagtgtcatc agtgtcatgc agccagccat cagcaacaga aagcaaagtc
     1381 cggtaatgtc tgtcccccgg atgatgtgcg taatggtggc ggtgagtgcc agaacaacac
     1441 agagcaggga gaggtgacgt ggaagccgga gggtatgcac ctgcatgaga taatgtccgg
     1501 cca
//
LOCUS       DAZGBC010000412         1503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGBC010000412 DAZGBC010000000
VERSION     DAZGBC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.414"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..927)
                     /gene="pilV"
                     /locus_tag="ACHK1J_005315"
     CDS             complement(<1..927)
                     /gene="pilV"
                     /locus_tag="ACHK1J_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO7914428.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNVRLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKC"
     gene            complement(932..>1503)
                     /locus_tag="ACHK1J_005316"
     CDS             complement(932..>1503)
                     /locus_tag="ACHK1J_005316"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO7914429.1"
                     /translation="AGHYLMQVHTLRRPRHLSLLCAVLALTATITHIIRGTDIAGLCF
                     LLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAFRLWQGDAAIALACALFWFLLLS
                     LLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTPWIIGIAAASTMICMSLFPRSNT
                     GAAIPFGPFICASLYIFTFFPGAIFHTGG"
BASE COUNT          365 a          396 c          385 g          357 t
ORIGIN      
        1 acactttgtg cctgccgtag ctgttttatc aagctgtaaa tactccccgg ttgaaagacg
       61 accgtcagac cggactgtac caccactgac ttttccgcct ttgatttccc cggtggttgt
      121 gatgcctttg ccattaaccg cgcgtatcca ctggctgtct gtcatggtga atccaccacc
      181 atgtgtgata ttcatccatc ccttattgtt tctggtgatt agccagccgt cttcactcgt
      241 aatatcacct ttcagtgtgg cactctggcc attgatattg ccggtgttgt ttatgttatt
      301 cccgcccata ttgattgcgg tatgcatctg attcagttca ggacgacctg ctacctgaaa
      361 acggtagaga cggtcacttt cctgcaggtc atcaccgctc atgacgacgg cgatgtgacc
      421 tgtcgaacag gtaagtccgt agtctcccgg tttatctgtc caggctccga atgcgcctgt
      481 tgccgttcca tttttgctga taaatccacc cagccccggc aactgacccg ccacactacg
      541 cagcccttct tctttgattg tctgccctcc agttgagcag gtcagggctt ccagacgccc
      601 ggtggtctgg ttacggcgga taccggtgat ataactctga ccaaagggag aatggttaaa
      661 cccctgtttc agatatccct gacgaatcag atcatcagcc gtgaaatttg ccggaaggct
      721 gccgctatgc agacggacat tgttgtcggc aatatagttt ctggctgcgg tattgtaggc
      781 atctgcatga tatgcagcac tctggtaaag ctgctcattc agccagtttt caatttctga
      841 ataaatcatt ggtgtggcga taccgatcac aatcagtccg gccagaattt caaacagaac
      901 aaaaccttta tcgtcgtctt tttgcatggt gttaccctcc ggtgtgaaaa atggcaccag
      961 gaaaaaaagt gaaaatgtag agtgatgcac agataaacgg accgaacgga atagcggccc
     1021 cagtattact gcgcgggaac agcgacatac agatcattgt gctggcggcg gcaataccga
     1081 tgatccacgg agtgctgttg gcagatgacc acatggccac acctgcaatc agaaacacgt
     1141 cccccagtcc cagcgtctcc actcccttgc ggtatgaggt gtagcgcctc aggagagata
     1201 acaggagaaa ccagaacaga gcacaggcaa gcgcaatagc agcatcgccc tgccataaac
     1261 gaaacgccac acccagcatc aggaatgtga gggtgaaggg acggggaaga cggcaggagg
     1321 ctgcatcagt cagtgtcatc agtgtcatgc agccggccat cagcaacaga aagcaaagtc
     1381 cggcaatgtc tgtcccccgt atgatgtgcg taatggtggc ggtgagtgcc agaacagcac
     1441 agagcaggga gaggtgacgt ggacgccgga gggtatgcac ctgcatgaga taatgtccgg
     1501 cca
//
LOCUS       DAZGBC010000413         1495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGBC010000413 DAZGBC010000000
VERSION     DAZGBC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.415"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHK1J_005317"
     CDS             <1..573
                     /locus_tag="ACHK1J_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914430.1"
                     /translation="ASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSFPYAGEWLT
                     EDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRLVVKESDHP
                     CWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILASGLVCDVLRIPDEPSRR
                     WFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            989..1414
                     /locus_tag="ACHK1J_005318"
     CDS             989..1414
                     /locus_tag="ACHK1J_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7914431.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
BASE COUNT          283 a          414 c          462 g          336 t
ORIGIN      
        1 gcctctgcca ttcagaaact gaccggaacc ggggcggaac ttgttctgtc cggcagtcag
       61 tggcagtcgc tgccggtact tccggcagac ggaacgcagg tgtctttccc gtatgccgga
      121 gaatggctga ccgaagacga aatcagggca gttcttgatg cggtgcacga tgcggtacgc
      181 agtgtcagtt gccgggtggc agaagatgcg cggcgtatcc gggcggcgct taccacaacc
      241 ggtcagacgt tgctgacccg ccagacgcgc cgctttcgcc tggttgtgaa ggaaagcgat
      301 cacccctgct ggctcgatga agacgacgaa aacctgcccg tggtgctcga tgccattctg
      361 aaccggggag cacgtttttc ctctgtggaa atgtacctgg tcagtgagtg tgtggagcat
      421 atcctggcca gtggtctggt ctgtgatgtg ctgcgtatac cggatgaacc gtcccgtcgc
      481 tggtttgaca gggatattct gcgggaggtt gttctggagg cacgggatga aatccgcagc
      541 atggcggatg ccctggcaaa aatccggaaa tgatgacgct ggcggggaaa aatctccgcc
      601 ttttcctgcc ggtccccgga cagagcacaa aggatgtcgc cgccggctcc gctttacccg
      661 gccatgcggg gcatggcctt gtgggttttc agttatgtgg cctcagcgtc gtgtgcgggc
      721 tgtgccgtgc ctccatcgta gccgggcttc tgcgggcggg ttgcaaggta aggtgaaccg
      781 ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt cacccgctca
      841 gttttcgcgg tctcaccctc caactcccgt cagccgtgtg cgtcaggctg cggcttccct
      901 tgcgcccttg caaccctgca ggcccggcaa agatggaggc acggcaccgc ccggtgtcgc
      961 ggaatatgtg aacaacggag gaaatcccat gcagtatgcg aaacctgtca ccctgaacgt
     1021 tgaagagtgc gaccgccttt cttttctgcc ttacctgttt ggcaacgatt ttctgtatgc
     1081 cgaggcgtat gtgtacgcgc tggcgcaaaa aatgatgccg gaatatcagg gaggcttctg
     1141 gcatttcatc cgcctgccgg acggtggcgg ttacatgatg ccggatggcg accgttttca
     1201 tctggtcaac ggtgaaaact ggtttgaccg cactgtgagt gctgatgccg caggtatcat
     1261 cctgacttcc cttgtgatta accgccagtt gtggctgtac cacgacagcg gggatgcggg
     1321 actgacacac ctttaccgga tgcgcgatgc gcagttgtgg agccacatcg aatttcaccc
     1381 cgaatgcaat gcgatttacg cggcactgga ctgattaacg gacggggcgg cgatgccgcc
     1441 ccagtaaagg agacgagaac atgtactgta ctgttaaaga aattatccgc gatgt
//
LOCUS       DAZGBC010000414         1491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGBC010000414 DAZGBC010000000
VERSION     DAZGBC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.416"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..619)
                     /gene="sctT"
                     /locus_tag="ACHK1J_005319"
     CDS             complement(<1..619)
                     /gene="sctT"
                     /locus_tag="ACHK1J_005319"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO7914432.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLGIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYA"
     gene            complement(621..881)
                     /locus_tag="ACHK1J_005320"
     CDS             complement(621..881)
                     /locus_tag="ACHK1J_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7914433.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(890..>1491)
                     /locus_tag="ACHK1J_005321"
     CDS             complement(890..>1491)
                     /locus_tag="ACHK1J_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7914434.1"
                     /translation="SGTCFIKFSIVFVIVRNALGLQQVPSNMTLNGVALLLSMFVMMP
                     VGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSYLIKYSEPELVSFFEKIQKVNGS
                     EDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVISSVLLTLGMMM
                     MSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          527 a          320 c          244 g          400 t
ORIGIN      
        1 aagcataacg tgccagcaat cccaggacag cctcaccgcc cagcataacg gcaatcacgg
       61 ggcttgcgta aacgatagta tgagtcatta tgttattaat atatgagaat aaaggctcta
      121 atttaggaaa attaaaatta cctgaaggcc ataaattata actttgccaa agagtttcca
      181 gaataaagtt cattccaccg ttagtaagat aaaccgcagc agagaaaagg ttaaataatc
      241 ttgctaattc cgaggtatcg acacctgtag ccggatccaa agtactactt aatgttgcac
      301 cgcgttggtt atcgataaag ctacctatac caaggaaaat ccaaaatggg gatgctaata
      361 agcatgcaat aaacagccct atgataattt ctctggcagc gaacaaagca atatcgattt
      421 catttaagtt caataattca tagtggtaat gctgaactaa tgcacatgat accactatta
      481 ttattggatg acggactact gttggtatat tcccactggc gaaaaatggt aacaaataga
      541 aagtgggtgc caatcggcaa aaaccgagcg ctgtagcagc cagcagacta tgcaactgat
      601 agagtatcgc ctcacccatg ctatcctctc gcgaatgcta aattcagcat ttcaatacca
      661 aaagaatata atttttcgcc ataccatccg ctcattaaaa aaaagcaaat acttacgcat
      721 aataatttca cgccaaatgg caacgtctgt tcttgcaact gtgttaccgt ttgaaaaagc
      781 cctactaata aaccaacaaa tgtcgcaact gcgataggtc ctgcagacat aactaaaatc
      841 agatacagcg ccctattccc tgcaaaaaca atatcgtcca tattttttct cagggattaa
      901 tggataggtc aaaatattgt aaaatgagtc cttttgataa cattgtccaa ccgtccatag
      961 cgacaaacag aattaatttt atcggtgtag atatggttac cgggctcatc atcatcatac
     1021 ctaacgttaa caaaacgctg gatataacca gatccactac aacaaagggt aaatagatat
     1081 aaaaacctat aataaatgca ctttttatct cactcaatgc atatgcgggg agtaacgaga
     1141 aaattgaaat attatcatca tcgattatct cttcattgtc ttcagaacca ttcaccttct
     1201 gaatcttttc aaaaaagcta accaactccg gttcagaata ttttatcaaa tatgatttat
     1261 aaccactcat gcctgtctca acaaaattaa caaccgacgc tacattattg aatgaaagat
     1321 tctcattctg actgttatta tatatttcct tcccaaccgg catcattacg aacattgaca
     1381 aaagcaaagc aaccccatta agtgtcatat ttgatggcac ttgctgaagt cccagcgcgt
     1441 ttcggacaat aacaaaaaca atcgagaatt taataaaaca tgtcccggat g
//
LOCUS       DAZGBC010000415         1485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGBC010000415 DAZGBC010000000
VERSION     DAZGBC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.417"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1440
                     /locus_tag="ACHK1J_005322"
     CDS             <1..1440
                     /locus_tag="ACHK1J_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7914435.1"
                     /translation="FSDKLARITIGINSDANEFVKKQPHSNRLQWSLASKKWKTSIDN
                     PIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLACIFGNGANYGV
                     HRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYWTINEDSPFGSV
                     DGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPNEYEGHFAFDLL
                     YNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNELFDVILESDKL
                     IIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRSNKTLAALREYD
                     RVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFRGGNDYQIDQWN
                     DCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRHILLGGNYSFED
                     QVAITNLDSLLEGVDPLSDIGDIESEYDG"
BASE COUNT          532 a          237 c          288 g          428 t
ORIGIN      
        1 tttagcgata aactggctcg tataaccatt ggaataaact cagatgctaa tgagtttgtg
       61 aaaaaacaac cacactccaa cagattacag tggagtctgg ccagtaaaaa atggaagacc
      121 agcatcgata atccgattta caatcagata aaaaatatcg gaatagttga cgtaatgaag
      181 tatgtaaaca atgagacgaa ttttttgtct gtgtttaatg ccatatcatc caggaaaaat
      241 aatcttgaag ctgactataa tgaccttctt gcctgtatat ttggtaatgg cgcaaattat
      301 ggtgttcaca gaatggcatc tgcatcagac cgtagtattg gaatactacg tgatgtgaat
      361 gacaaattca ttcgtccaga aacaacagga atggccaaca cagtaatatc tgatgcaatt
      421 gcaaatcttt ctgtatataa atactggacg ataaatgaag attctccatt tggaagtgtt
      481 gatgggcaga agcacacttg cagaataaat acatttaaag cccgttattc tgccaagtat
      541 ttcagaaagg ggaaaggggt ttctgcgatg acactggtat ctaatcatgt gccattatca
      601 actgtggtaa tttctccaaa tgaatatgaa ggtcattttg catttgactt gttatataac
      661 aacaccagtg aaatacaacc aaagtcatta gcgactgaca atcatggtat aaacaacgtt
      721 aattttgcga ttcttgacat ttttggctat cagttttcac cacgttatgc aaaattcaag
      781 aaaatattta atgaattatt tgatgttata ctggagagtg ataaactcat tataaaactt
      841 aaaaaacaat ttaactttaa gctcattgaa catgaatggg aaaacatcca acatattatt
      901 tgttctttaa gcagaaaaac aaccattcaa agtacggttg ttaagaagtt atctaataca
      961 aagcgtagca acaagacatt agcagcactt cgggagtatg accgagttat aaaatgcata
     1021 tatgtcctgg attatgctga caataaaacc ttaaggcaat ttgtgcaaca agcactgaac
     1081 agaggtgaag cttatcatca actaagaaga gcaatagcat cagtaaatgg aaatcaattt
     1141 cgcggtggta atgactacca aatagatcaa tggaacgact gtgcgaggtt aatagctaat
     1201 tgtgtaatct attataattc agcaatatta tccaggctgg tcgagaaatt tgaaaaagaa
     1261 aataataaaa aagcgataga tgttctggct aacttatccc cagtagcatg gagacacatt
     1321 ctccttggag ggaattattc ttttgaagat caagttgcga tcacaaattt ggacagtctt
     1381 ttggagggag tagacccact aagtgatatc ggagatattg agtccgaata tgacggttaa
     1441 caacatgata aataatggtt tttatgctaa gagtgagact tttgg
//
LOCUS       DAZGBC010000416         1433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGBC010000416 DAZGBC010000000
VERSION     DAZGBC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.418"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..641)
                     /locus_tag="ACHK1J_005323"
     CDS             complement(<1..641)
                     /locus_tag="ACHK1J_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO7914436.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAPH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKT"
     gene            complement(989..>1433)
                     /locus_tag="ACHK1J_005324"
     CDS             complement(989..>1433)
                     /locus_tag="ACHK1J_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914437.1"
                     /translation="IATYDESIATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFP
                     RDGADIFPDGWLENMTRRAERVFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQ
                     ASCHHSGCHPDVSVYTERCAQETSLSPVHPTRQCPVQSTDSAETE"
BASE COUNT          356 a          396 c          373 g          308 t
ORIGIN      
        1 gttttcccca ttgacggacg ggcagccagc aggaccaggt cacctggctg cagaccacag
       61 gtcatttcat ccagacagtc aaatcccgtg ggggtaccgg tcacaccacc ggagccgagc
      121 acactttcca gttgctgcag aacggtatcg agcgcggatg aaagagaggc ttccgtgcgc
      181 tcccggagcg tcctgttctg cgcaagcgca aacagacgcc gctctgcagc atccagcaca
      241 tcccgtgacg tggcatttgg tgccgccaca tcggcaatca gggtgtttcc ggtggcctgt
      301 aactgacgca gaacgctttt ctccaccaca atgcctgcat aggccatgat gttggccgca
      361 gaaggtgtgt tcttgctgag ctcggccaga taagcaaaac cacccagtac gtccagcctg
      421 ccctgatgtt ccagatgctc tgacagtgta atcaggtcca gaggctggcc attcaccgcc
      481 agttcagaca tggcacggaa gatggcccgg tgcggagcca cataaaaatc gtcaggactc
      541 agcatcagca ccacttcatc ccagcgttca ttctccagca tcagcccacc cagaacggcc
      601 tgctctgctt cgtatgcaca gagaacagac atttcagcca tggcgtaccc tccctctctg
      661 actgccggaa acctggctac gtgaaaaaag gaaaaagggt gccggacgta cagttgtcgg
      721 gaataaaatc ccggtaactg tctgtacgtt ttcagaccgg tgagtttgcc gaccccggtc
      781 agaaggtacg gacagcattc gttcattcat aacaagttgt tttaaaggaa aaagtttatg
      841 aatacagaaa aacaactgcc gtccggcatt gaatctgagg tgaaatacac cctgcagttc
      901 tggctgctga gtaacggaaa ggtcatgacg gaaaaagacg ggctctgtaa cccctttccc
      961 ctgaactgta ttgaatcggc tcatcctttt actccgtttc agcagaatca gtggattgaa
     1021 ctggacactg tcgggtcgga tggaccggtg aaagcgacgt ttcttgtgca cagcgttctg
     1081 tataaactga aacgtcaggg tggcaccctg aatgatggca actggcctga atacggccgg
     1141 tgcattgctc actggcacac tgacgtgttt gttgagaaaa aagcgctgcc tgactggctg
     1201 agagcattcc acactgacgt ttcgcactcg taaaaacacg ttcagcccga cgggtcatat
     1261 tttccagcca cccgtcgggg aaaatgtcag caccatcacg gggaaacaaa atcgctttcc
     1321 attcagggta tgagcgcgga atacctccgg aagcattccg gctcagacag cgactcacag
     1381 tgcaggcagc acggaatgca tatgttgcga tgctctcatc atatgttgcg atg
//
LOCUS       DAZGBC010000417         1430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGBC010000417 DAZGBC010000000
VERSION     DAZGBC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.419"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..449
                     /locus_tag="ACHK1J_005325"
     CDS             <1..449
                     /locus_tag="ACHK1J_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7914438.1"
                     /translation="LVKKVLFSYPLILCCSPDYINKYGIPSNPFSLKHHNCLYASSLV
                     GGNCWHFYIRDEITTLEIAKTVNIENSEFLKAVALMGGGIAYLPYPLVQSEILSGKLV
                     SIMNDYIKTRFEVSLWFRKPLSLRCVTFRNFLVERASQLKDTVQFR"
     gene            623..1430
                     /locus_tag="ACHK1J_005326"
                     /pseudo
     CDS             623..1430
                     /locus_tag="ACHK1J_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          431 a          305 c          332 g          362 t
ORIGIN      
        1 acttagtaaa aaaagtgttg ttcagctatc cattgatact atgctgttct cctgactata
       61 taaataaata tggaataccc tccaatccat tcagtttaaa gcatcataac tgtttatacg
      121 catcttctct tgttggaggg aattgttggc atttctatat tcgtgatgaa ataacaacat
      181 tggaaatagc taaaacagta aacattgaaa atagtgagtt tttaaaagca gttgcactaa
      241 tgggtggggg gatagcatac ttaccatatc ctcttgttca atcagaaata ctgtcaggaa
      301 aactagtatc aataatgaat gattatatta aaactcgttt tgaagtaagt ctgtggttta
      361 ggaagcccct atccttgcgt tgtgttacat ttaggaattt cctagtagaa agagcctcac
      421 aactcaagga tactgtacaa ttcagataaa tcacttaact tgatgcatca atcacccagc
      481 gaccagttat gctcatcata atcattattc aggtgtgagc ataatgagca taacccagcg
      541 atccagacag gttccctgaa tctccagaca accaatatca attaaataag tgatagtcta
      601 aaaactagtc attagagaac agatgattga tgtcttaggg ccggagaaac gcggacggcg
      661 taccacacag gaaaagatcg ctattgttca gcagaacttt gaaccgggaa taactgtctc
      721 cctcgttgca cggcaacacg gtgtgacagc cagttatttc tctggcgtaa gcaataccag
      781 gaaggaaatc tcactgctgt ggctgctgga gaacaggttg ttctggcctc tgagcttgct
      841 gccgccatga agcacattaa agaactccag cgcctgcccg gcaagaaaac gatggaaaat
      901 taactcatta gagaagccgt tgaatattgg cgtgcaaaaa agtggatagc gcacgcccaa
      961 tattgcccgg gaatggggag taagtttcgt cagccgttat ctccgtgtgt tgcgtgcgca
     1021 gttgcacgtc attctcagac gaaccgatga ctggaaggat ggccgccgca tgatacggat
     1081 gtttatctcc gtaataacat ggtgtacacc atgttatcgg agagctgccc acgtatggtt
     1141 atcgtcggct gtgaaacagt acaggacgtc atgctgggag cggtagaaca ccgcttcggc
     1201 agcgaccttc ctgcgtctcc agtggagtgc ctgacggata atagttcatg ctatcaggct
     1261 aatgaaacac gccagttcgc ccggatgttg gaacttgaac cgaagaacac ggcggtgcgg
     1321 tatccggaga gtaacggcat agcagagagc ttcgtgaaaa cgataaagcg tgactacatc
     1381 agtatcatgc ccaaaccaga cgggttaacg gcagcaaaga accttgcaga
//
LOCUS       DAZGBC010000418         1429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGBC010000418 DAZGBC010000000
VERSION     DAZGBC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.420"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..327
                     /locus_tag="ACHK1J_005327"
     CDS             <1..327
                     /locus_tag="ACHK1J_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914439.1"
                     /translation="TKYLHVFFFGFPDEYDNFLFKASYLKLIYIVFLDDFDVTYNDKN
                     YSIEKEQLIRDEINKEHLTAHQNRINVAEVADSDKSEFSRIKTSSDFDGALWTGIRKL
                     ISFFKK"
BASE COUNT          409 a          254 c          304 g          462 t
ORIGIN      
        1 accaaatatc ttcatgtttt cttttttggt tttcctgatg agtacgataa ttttttattt
       61 aaggctagtt atttaaaatt gatttatatc gttttcttag atgattttga tgtgacttat
      121 aatgacaaaa actacagcat agaaaaagaa cagctaatta gagatgaaat taacaaagaa
      181 catttgacag ctcatcaaaa tagaataaat gttgctgagg ttgctgattc tgacaagagc
      241 gaatttagta gaattaaaac ctcttcggat tttgatgggg cattatggac tgggataaga
      301 aagcttattt cattctttaa aaaatagatc tatagcgtat cgttccggcg aactcatcct
      361 gatgcgtttt cgggggtcca tcggtgaaac atacgaatga tcgccctctg acgatggatg
      421 atttattttg ttgcacagaa gcgatcgctg ggcaggtacc ggaggttggc tctgaagctc
      481 taattgcgtc aggttgcgaa gtgttgacac gggtattggt gagaactgcc ccgggaacga
      541 ctctgggctg attctggttc tgattcgctg tcagcccgtg tggttactgg cctggctgat
      601 ttcctgtcgt caggcgttta actcccgact gcaggtatca cttaccaaga gaaggtaaat
      661 ccttttagac gtacagtgaa agttgtctgg tcataatctg aagcatctga caaagttgtg
      721 tccaccaaat aataagtagt gggaaccaaa gtgtcatata tacagaaaaa tataactcac
      781 cgatttcctt cacgttaata cgcaggggga ggtatataac agtccgttca ggtattggat
      841 aaatatttag agtggaatga ttacagttat actgtggatg ggagaagata tttactgtta
      901 attgattatc atttttaatt tgtattataa taacaaggtc ccgtaaattc cggactacag
      961 agtccagcca gtttgctcat ccgtcaattc aggtagatgc gtcaggcttt gtggctggcg
     1021 gaaaatggct cgttcgctga tattgttgcg tcatacaaaa acagtacgtg atgagtatga
     1081 ggctttttta tctgtatata atctcttatt ctgtaaatta ggatagggta atctcgcaat
     1141 catcctcttc ccatatcacc agtatatgct gagccgtgtt tgtctatttg gttaaaattc
     1201 gctacaatcc tattctctct tttgggaata taaaagtctc cattttctct ttattcgtta
     1261 atggagactg tatttaaagg cagaaatcag gagtgattga caggatacct gtcagcaatc
     1321 aatacaattc agattccagt tttccgttcc ctacaatttt atttagtccg ggagagttca
     1381 atctctccag acataacgaa aacagtttaa cgcctgtatc cagcacatt
//
LOCUS       DAZGBC010000419         1423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGBC010000419 DAZGBC010000000
VERSION     DAZGBC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.421"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..>1423
                     /gene="yjiT"
                     /locus_tag="ACHK1J_005328"
     CDS             26..>1423
                     /gene="yjiT"
                     /locus_tag="ACHK1J_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048814575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjiT"
                     /protein_id="HFO7914440.1"
                     /translation="MGQSEYISWVKCTSWLSNFVNLRGLRKPDGRPLYEYHATNDEYN
                     QLTQLLRAVGQSQSNICNKDFAACFVLFCSEWYRRDYERQCGWTWDPIYKKIGISFTA
                     TELGTIVPKGMDDYWLRPIRFYESERRNFLGTLFSEGGLPFRLLKESDSRFLAVFSRI
                     LGQYEQAKQSGFSALSLARAVIEKSALPTVFSEDTSVELISHMADNLNSLVLTHNLIN
                     HKEPVQQLDKVHPTWRSEFPIPLDDETGTHFLNGLLCAASVEAKPRLQKNKSTRCQFY
                     WSEKHPDELRAIVSLPDEVSFPVTSEPSTTRFELAICEDGEEVSGLGPAYASLENRQA
                     TVRLRKSEVRFVRQNPSAGLSLVARAGGMIVGSIKLDDSEIAIGEVPLTFIVDADQWL
                     LQGQASCSVRSSDVLIVLPRDNSTVAGFDGQSRAVNVLGLKALPVKGCQDVTVTANET
                     YRIRTGREQISIGRFA"
BASE COUNT          395 a          297 c          337 g          394 t
ORIGIN      
        1 atcttttatc tctaaggagc ttgctttggg tcaatcagaa tacatttcat gggtaaaatg
       61 tacttcctgg ctaagtaact ttgtgaatct tagagggttg agaaaaccgg atggccgtcc
      121 tctttatgaa tatcatgcaa ccaatgatga atataaccaa ttaacgcagc tactccgtgc
      181 agtcggtcaa tcacaatcta atatatgtaa taaggacttt gctgcctgtt ttgttctttt
      241 ttgctctgag tggtaccgca gggattatga acggcaatgc ggatggacgt gggatcctat
      301 atataaaaaa atagggattt catttaccgc cacagagctt ggtactatcg taccgaaagg
      361 gatggacgat tactggctgc gcccaattcg attttatgaa tctgaacgac gcaattttct
      421 cggaacatta ttcagtgaag gtggtttacc tttccggtta ctcaaagaat cagacagtcg
      481 ctttctggct gttttctcca ggatccttgg acaatatgag caagcaaagc agtcaggttt
      541 ttctgccctt tcgctggcaa gagccgtcat agaaaaatca gcgctgccaa ctgtttttag
      601 cgaagatacc tctgtagaac ttatcagtca tatggctgat aacctcaatt ccttagtact
      661 tactcacaac ctaataaatc acaaagaacc agtacaacag cttgataagg ttcaccctac
      721 ctggcgaagc gagtttccaa taccgttaga tgatgaaaca ggaacgcatt ttttgaatgg
      781 actgctatgc gcagcctctg tagaggctaa gcctcgacta caaaaaaata agagtacgcg
      841 ttgccaattc tattggtcag aaaagcatcc agatgagctt agggcgatag tatctctccc
      901 tgacgaagtt tcctttcctg taacaagcga gccgtcaact acgcgctttg aacttgccat
      961 ttgtgaagat ggtgaggaag tctctggcct tgggccagcc tatgcttccc tggaaaacag
     1021 acaggcaaca gttcgattac gtaaaagcga agtgagattt gtcaggcaaa atccatcggc
     1081 aggtttgtcg ttagtggctc gtgctggagg gatgattgtt gggagcatta aacttgatga
     1141 cagtgaaatt gccattggtg aggtgccgtt aaccttcatc gttgatgcag atcaatggct
     1201 gttacaggga caggcctcct gcagtgtgcg aagcagcgat gttctgattg tgctccctcg
     1261 ggacaatagc actgttgctg gttttgatgg ccaatcgaga gcagtaaacg tattaggact
     1321 taaagcgcta cctgttaagg gatgtcagga cgttacagtt acagcaaatg aaacttatcg
     1381 gattcgcacc ggaagagaac aaatcagcat cggaaggttt gct
//
LOCUS       DAZGBC010000420         1397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGBC010000420 DAZGBC010000000
VERSION     DAZGBC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.422"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..569)
                     /locus_tag="ACHK1J_005329"
     CDS             complement(<1..569)
                     /locus_tag="ACHK1J_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HFO7914441.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHT"
     gene            complement(566..1354)
                     /locus_tag="ACHK1J_005330"
     CDS             complement(566..1354)
                     /locus_tag="ACHK1J_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914442.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQVFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEAQLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEVLSALPEKALPRSWWRRLLQRGTRS"
BASE COUNT          303 a          450 c          358 g          286 t
ORIGIN      
        1 atggtgtgcc ccacacgttc acccagtgcc agcgtcacat cctgctcatc cggctcaata
       61 ccatgcaggg caggatttcc tcccaccggc ggcaggggac ggacgggatt cagcggcgag
      121 tcacgacgaa gaagctgccc aatccaggga agactgtgct ccgttttcat ttcaagccag
      181 cgcgctgtcc ggtacagact gcccggctgc agatagtgtg caacctccgg tcgcagcgtg
      241 tcctgaaggc gctgggtgtg cttctgtgtc cagcggaagc cgcgtcccag aaacagccgc
      301 tgacggctga ccggaatttg ccgggaactc atcacataac ggggcagccg acgcagattg
      361 cgacggtaac ggatgatctt catcccctgt cggaaacgga ccactgccag aacgccaaag
      421 ccggcggctg tgacatagct gaccgaaggc gcgagcgcca ccgcccaggg cgctgtcaca
      481 cagacaccgg ccgccgttgc ggccaccacg gcggtattca gttccacagc cggacgcagc
      541 agagcctcaa tcacataacg gttactcatg accgggtacc tctctgtaaa agacgccgcc
      601 accatgagcg cggcaacgct ttctccggta atgctgacag tacctccagt ctgcgctgta
      661 acaccatcac ctcccgccat gcgtctgccg cattcagcag taatgcctta tacaccagtg
      721 gcccgtcatg tccgccggca tgcacatcat ccactgtcaa ctgcgcctct gttctgaaac
      781 gatccacttt ccagcaggcc aggtcttcgc gggaaacaca ctgccgccgg atctgcagac
      841 gaagatacac atcatgccgg gacaacggtt ctgccaggtt gtgaatttcc cgacaggcag
      901 accggaacac ctggtctgtg tgataaatac tctgggccac attgtgaagc tgtgtgcgcc
      961 cctcaccctg caggtggaac agaaagcggg ccagacagct gcggtgatta ccgtcagcac
     1021 tgacatacca gtgaatgcca tccgtgctga caaaacccat cccggcatgc tgttgctctg
     1081 cctgcgtgta atatcccgga ttcttttcaa gtaacgggat attgatgtcc atgcgtttac
     1141 cacgctccag cagctccagc cagctcattc cggcatactg tggatggtca gtccccacaa
     1201 tgcggtacac attgatactg ccctgtcccg tccagtagtg ttccgtaata aaatccgaca
     1261 ttgccgcaaa ctctgcggta tcccatgcaa ccagttgttc atgtgcccag gccggagcat
     1321 tcgcctgtat ccgctctgta aatgccccct tcattcagct cttctctcct gcacttttga
     1381 gataccgata ccgtcag
//
LOCUS       DAZGBC010000421         1385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGBC010000421 DAZGBC010000000
VERSION     DAZGBC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.423"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..857
                     /locus_tag="ACHK1J_005331"
     CDS             9..857
                     /locus_tag="ACHK1J_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO7914443.1"
                     /translation="MNNTLLPSINIPCTLFETISLFNDYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKIFS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYA
                     IGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIE
                     RIENYL"
     gene            854..1345
                     /locus_tag="ACHK1J_005332"
     CDS             854..1345
                     /locus_tag="ACHK1J_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO7914444.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGSSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNLHDFKK
                     DNF"
BASE COUNT          491 a          238 c          207 g          449 t
ORIGIN      
        1 aaatgtaaat gaataacaca ttacttccat caattaatat tccctgtaca cttttcgaaa
       61 ctattagttt atttaatgat tattctgctg atgatatgca atatggtgat atggtagaac
      121 aagattttct ctcactcggc ctaagcgata tctcagcaaa agttgatcct tacagactta
      181 ttaaatacca tttccctggc ccaggttcta ttaatgttgc ttttagtgcc tcaagttccg
      241 gaacaaaaat atcacaacgt gaatgcactg atattttatt tgcagaaatg aaagaactag
      301 caaaaatttt ttcctttttt ggacaatata aaacacttat cgaagatctg attgaacatt
      361 ttagatatgg aaatggcagc aattttcata gccaacaatt aaatttatct tttcatgaaa
      421 aaataaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      481 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacaa
      541 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      601 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      661 ctataggatg gagtgcgaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      721 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      781 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      841 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      901 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      961 tggcagttca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
     1021 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
     1081 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
     1141 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
     1201 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1261 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatctgca
     1321 tgactttaaa aaagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1381 ctcat
//
LOCUS       DAZGBC010000422         1378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGBC010000422 DAZGBC010000000
VERSION     DAZGBC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.424"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..177
                     /locus_tag="ACHK1J_005333"
     CDS             <1..177
                     /locus_tag="ACHK1J_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914445.1"
                     /translation="NDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTS
                     ADEILAYRVGWAQA"
     gene            253..435
                     /locus_tag="ACHK1J_005334"
     CDS             253..435
                     /locus_tag="ACHK1J_005334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914446.1"
                     /translation="MTMLVYGVKTATEMLILYVMLILFPEHPVDVNNFQIFGSESLDI
                     SIYATVDVFCGAWQCD"
BASE COUNT          405 a          253 c          333 g          387 t
ORIGIN      
        1 aatgatgtgg aggtgacagc agaagagctt atagcgctga gtgaagcggc agagcaggcg
       61 atgttcacca aagggatgga aatccacgtt cgccagcgca ccatgaagaa ggagctggaa
      121 aagctgacca gtgcggatga gatactggca tacagagttg gctgggcaca ggcgtaataa
      181 aaaagggggg ctgataaaca gcccctgacc aaagaaaagt atcagcggaa acatttagaa
      241 ggatctaaat aaatgaccat gcttgtatac ggcgtaaaga ctgccacgga aatgttaatt
      301 ctgtacgtga tgctgatctt gtttccggag catcctgttg atgtgaataa ttttcagatt
      361 ttcggaagtg agagtttgga tatttccata tatgccacag ttgatgtgtt ttgtggcgcc
      421 tggcagtgtg attaagggaa cagggataac gaaaaaagcg ggggtatcac caccccccgg
      481 tacaaggaag tattaactat aatggaaaca ttaatgaaga cattcagtgc tatgttatat
      541 ggcaaataaa gactgccaca gggaagtttg cagaaacagt gatgccgatc gtgtttctgt
      601 gctcttctct gataagagta atttttcaga tttccggaag tccgtatttt gcacgggccc
      661 ggttgtatgc ttcagactgc ggtggatatc aaggtcacgg agttggtcgg ttgcaaggaa
      721 tagattgaga attgagcgtg tttctggcac atgcctgact gtaatcatcc ctacaaacta
      781 cattgatgcc agaaacgcac tggtaacagg gcatcgtgta tttcagtatg tccagttttt
      841 gttgcggtga ttgcataaaa aatccccctt gagggttgaa atatcaccgg aattttcggc
      901 aagtattttt atcgggttga gtgtatttct ggggctctgc tgaggcaatt cactgaatgt
      961 ggcttttgtg ctcgctggaa atgcactcat acacttttta acgcgatttt tttcaggcaa
     1021 tagtttttaa tttgttagcg ttctcgctgt caggcacgtt ttgagcacgt ttccgactct
     1081 cagctacagc aatccacgac tagggacagc acttacgttt tacttagcgc accgtaaaac
     1141 tccgctcttc agggtaaggg cttacatgaa gaaaaaaaat aaacttgtga atatgaaagt
     1201 aaatactata aaaagatggt tcctcttttt tctcacgtgc tgaataatat aaacaatagt
     1261 gcatgataca aaagacacta ataaccacaa agttatgata cggagcacga tttatgtctt
     1321 cctgattcag tgtgtcaaga aacagttatc aatatacaat aactgtttct tttattat
//
LOCUS       DAZGBC010000423         1375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGBC010000423 DAZGBC010000000
VERSION     DAZGBC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.425"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1375
                     /gene="iha"
                     /locus_tag="ACHK1J_005335"
     CDS             <1..>1375
                     /gene="iha"
                     /locus_tag="ACHK1J_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO7914447.1"
                     /translation="SSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQ
                     RQGSSVTSLSDTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDG
                     QLGSLTGGYDHTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDK
                     WGLAGKPRELKESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQK
                     SWSVFAEDEWHLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGY
                     KAPTMGQLHKGISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFIT
                     DFSNKIVSYSIDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDG
                     DNKGAPLSYTPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYD
                     EKGEYLKA"
BASE COUNT          365 a          316 c          377 g          317 t
ORIGIN      
        1 ctcttccgtc aacgccgggc tgaatctgca ggagagtaac aaatggggca acagcagcca
       61 gtttaatttc tggagcagtg gccctcttgt ggatgattct gtcagtctgc agatccgcgg
      121 cagcacacag cagcgtcagg gctcatcggt cacatcactg agcgacacat cagccacgcg
      181 tattccttat cccacggagt cacagaatta taatcttggt gcgcgtattg actggaaggc
      241 tacggagcag gatgtgctct ggtttgatat ggataccacc cgacagcgtt acgataacca
      301 ggatggtcaa ctgggtagcc tgacaggggg atatgaccat actctgcgct atgagcgaaa
      361 caaaatttcg gctggctatg atcatacttt caccttcggt acctggaaat cgtatctgaa
      421 ctggaacgag acggaaaata aaggtcgtga gcttgtacgt agtgttctga agagcgataa
      481 atgggggctt gccggtaaac cgcgggagct taaggaatct aaccttatcc tgaattcatt
      541 gctgcttacc cctctgggag aatctcatct gcttactgtg gggggagagt atcagagttc
      601 gtccatgaaa gatggggttg tccttgccag cacaggtgaa actttccgac agaaaagctg
      661 gtcagtattt gctgaggatg agtggcatct cacggatgta cttgcgctga ctgcaggcag
      721 tcgctatgaa catcatgatc aattcggggg acacttcagt ccgcgtgcat atctggtctg
      781 ggacgtgacc gatgcctgga cgctgaaagg cggtgtgacc acgggatata aagcccccac
      841 catgggacag ttacataagg ggatcagcgg tgtatccggg cagggacgta ctaatctact
      901 cggtaacccc gaactgaagc cggaagagag tgtaagttat gaggcggggg tatattacga
      961 taacctctcc ggtctgaatg ccaatataac aggctttata acagacttct ccaacaagat
     1021 tgtctcttat tccattgatg ataacaccaa tagctataca aacagcggaa aggcccgctt
     1081 gcatggtgtg gaattcgccg gcacattgcc gctgtggtca gaggatgtca ctctgtcgtt
     1141 gaattacacc tggacccaaa gtgaacaacg tgatggtgat aacaaaggtg caccgctgag
     1201 ttatacccct gaacacatgg tgaatgcgaa actgaactgg cagatcaccg aagaggtggc
     1261 atcatggctg ggtgcccgtt atcgcgggaa aacaccacgt ttcacccgga attattcgtc
     1321 actgagcgct gtacagaaga aagtgtatga tgagaaagga gaatacctga aagcc
//
LOCUS       DAZGBC010000424         1373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGBC010000424 DAZGBC010000000
VERSION     DAZGBC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.426"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1373
                     /locus_tag="ACHK1J_005336"
     CDS             <1..>1373
                     /locus_tag="ACHK1J_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7914448.1"
                     /translation="TLTLDREVTLPETGTSTVNLINGSGKPVRVDITAHPAPDRIQVS
                     ALPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARF
                     EPLSGSLNSVIPPAVQHLTVEVSASDGQYMALAKWDTPRVVKGVRFSLRLTSGSGENS
                     RLVTSAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTAFRINAPAAPASIELT
                     PGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKP
                     GKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGMWELIDNSQ
                     LDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQK
                     TKNGIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMND
                     VFLKRLFA"
BASE COUNT          319 a          367 c          431 g          256 t
ORIGIN      
        1 acactgacgc tggaccgtga ggtgacactg ccggagacag gtacatcgac ggtgaacctg
       61 attaacggca gcggtaagcc ggtgcgcgtg gacatcactg cacaccccgc cccggaccgg
      121 atacaggtca gcgccctgcc ggatggcgtg gagacatacg gtgtgtgggg actctccctg
      181 ccgtcactgc gtcgtcgcct gttccgctgt gtttccatcc gggaaaacac ggacggcacc
      241 tttgccatca cggcagtgca gcacgtaccg gaaaaagaag ccattgtgga taacggggcc
      301 cgctttgagc cgctctccgg ttcactgaac agcgtcatcc cgccggcagt gcagcacctc
      361 acggtggagg tgagtgcctc agacggccag tatatggcgc tggcaaaatg ggacacgccg
      421 cgggtggtga agggcgtgcg cttcagtctg cgcctgacca gtggcagtgg tgaaaacagc
      481 cgcctggtga ccagcgccat cactgccgac acggagcacc gtttcagtgg cctgcctctg
      541 ggggaataca ccctgacggt cagggcgata aacagctacg gccagcaggg cgaacctgcc
      601 accaccgcgt tccggattaa tgcgcctgca gcaccggcca gcattgagct gacgccgggg
      661 tattatcaga taacggcggt cccgcatctt acggtgtatg acccgacggt acaatttgag
      721 ttctggtttt cggaggcaaa aatcgcagac acatctcagg tggaaacctc tgcccgttat
      781 ctggggacag gcagccagtg gagtgtctcc ggcccgcaca ttaagccggg gaaggatttc
      841 tggttttatg tgcgcagcgt caacctggtg gggaaatctg cgtttgtgga agccagtggc
      901 cgggccagca atgatgcgga agggtatctg gggctgtttc gggaaaagat aggaaaactg
      961 catctggcac aggggatgtg ggagctgatt gataacagcc agcttgatga tgagatggcg
     1021 gagatgaaga ccaccatcac agaaacccgc aatgaaatca cacagacggt cagtaaaacc
     1081 ctggaagacc agagtgccac catacagcag atacagcggg tgcagacaga cacaaataac
     1141 gacctggctg cgctgtacat gctgaaggtg cagaaaacaa aaaacggcat tccgtatgtt
     1201 gccggtatag gtgcggggat tgaggatgct gatggccagc ccctgagcaa tattctgctg
     1261 caggcggacc gtatcgcgat gattaacccg gagaacggca acaccacgcc gctgtttgtg
     1321 gcgcagggga atcagctgtt catgaacgat gtgttcctga agcggttgtt tgc
//
LOCUS       DAZGBC010000425         1368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGBC010000425 DAZGBC010000000
VERSION     DAZGBC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.427"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            143..1354
                     /gene="bluF"
                     /locus_tag="ACHK1J_005337"
     CDS             143..1354
                     /gene="bluF"
                     /locus_tag="ACHK1J_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO7914449.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          384 a          294 c          296 g          394 t
ORIGIN      
        1 gatttttcgc caactcgtgt tcaacttttt ccattttcta tcaatgtaaa gaaacagtta
       61 tgttttggta ttaaaagaaa gacaccggaa gatatgtctg ttaccgtctt actctcgcct
      121 ctcccattac cctggacagt taatgcttac cacccttatt tatcgtagcc atatacgtga
      181 cgacgaacct gtcaaaaaaa tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca
      241 gtctgacgta acagggatct tactgtttaa tggttctcat tttttccagc ttctggaagg
      301 tccggaagaa caggttaaaa tgatatatcg ggctatatgc caggatccac ggcactataa
      361 tattgttgag ctgctgtgcg attacgcgcc tgctcgccgt tttggcaaag cgggaatgga
      421 attatttgat ttgcgcctgc acgagcgaga tgacgtttta caggccgtat tcgacaaagg
      481 cacatcaaaa tttcagctaa cttatgatga cagagcgcta caattttttc gtacttttgt
      541 ccttgcaacc gaacaatcaa cctatttcga gatccctgcc gaagactcct ggctttttat
      601 cgctgacgga tctgataaag aacttgattc ctgtaccctt tcaccaacta taaacgacca
      661 ctttgccttt catcctattg tcgatccctt atcgcggcgg ataatcgctt ttgaagccat
      721 tgtgcaaaaa aatgaagaca gcccgtcagc tatagcggtt ggtcagcgta aagacgggga
      781 aatctacaca gcggatctta aaagtaaggc gcttgcattc gcgatggcac acgcacttga
      841 gctcggtgat aaaatgatat caatcaatct attacctatg accctggtta acgagcctga
      901 cgcagtctct tttttacttg atgaaataaa ggccaatggt ctggtgcctg aacaaatcat
      961 cgttgaattt actgaaagtg aagtcatatc tcggtttgat gagtttgccg aagcggttaa
     1021 atcgctaaag gctgccggta tcagtgtagc aattgatcat tttggcgcag gttttgctgg
     1081 tttgttactc ctgtcacgct tccagcctga cagaattaaa atcagtcagg aattgattac
     1141 caatgttcat aaaagcggac cacggcaggc aattattcag gcgatcatga aatgctgtac
     1201 atcacttgaa attcaagtct ctgctatggg cgtggcaaca ccagaagagt ggatgtggct
     1261 tgaatccgca ggaattgaga tgtttcaggg ggatctgttt gctaaagcta aattgaatgg
     1321 tatcccttcc atagcgtggc cagaaaaaaa ataattttca gcacataa
//
LOCUS       DAZGBC010000426         1348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGBC010000426 DAZGBC010000000
VERSION     DAZGBC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.428"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(274..1278)
                     /locus_tag="ACHK1J_005338"
     CDS             complement(274..1278)
                     /locus_tag="ACHK1J_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO7914450.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTINQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
BASE COUNT          334 a          346 c          303 g          365 t
ORIGIN      
        1 tctatggtcc ccaccatttt tgcaacactg attttgattg atgttggctt gcctaaatct
       61 atccggcgtc attatgggca tggatgcccg cgcctcgatg agttccgcac ctgatgagcc
      121 caaaaaacca tccggcttca gtgagccatt ttcttgctga ggcttttcat caggccgata
      181 tgccgttcat gtcatcaata atcagtcttc gcaaaaccag attgggaact actttaattc
      241 atgattaacc tgccagccag actggcgcca tcgttacctt tcgaacacag tctgctgtgt
      301 tgtgattgcc cacgctatcc gggctaattt gttggccagt gcgcaacata ctacgctgga
      361 gtgttttctt gccaacaacg ccctgaccca atccgccaac cttcctgact gattctcgat
      421 tctctgaata aaggatcttg cgcactgaac gagtaaccgt cgcagatttt tatctccacg
      481 tttacttatc cctaaaagtg tatttttacc acctgtgctg tactgccggg gtaccagacc
      541 ggttgaagca gcaaagtccc gactgcagct atattgttta ccgtctccca gctgcgatga
      601 cagtacgctg gcggttatcg gaccaatacc gggtattgtc tgaagacgtt gtcctacctc
      661 atcacgactc agttcctgct ccagtgcgat ttcaagctcg gtgatctgtc cgacaagata
      721 aatataatgg gcatgtaagc gcatcagtaa ctgcgccaga tagggaggaa gttcgttctc
      781 tgcaagtact gttgaaagtc gttttaccac tgcgactcct gtcggcatac tgatgccaaa
      841 ttccagcaaa aaagcatgca tctgattgat cgttttgact ttatcactga ccaatgattc
      901 cctgacccgg tgaagcgccc gcatggcctg ctgagcttct gttcgtggtt gcacaaatcg
      961 catggaggga cgagatgccg cttcgcatat ggcttctgca tcgacaaaat cgttcttgtt
     1021 gctcttaaca aaagggcgaa cgaactgcgg agaaatcaat tttgcttcat gacctaaatc
     1081 agcaatccgg cgagccataa aatgagctcc ggcgcaggct tccatcacaa cggtagacga
     1141 ggcacagcct gccagaaact caataagttt ggtacgggta aactttttac gcagcaatac
     1201 cttgcctgac ttatcttggc agtgaatgtg aaaggaatgc ttgccaagat ctataccaat
     1261 aagcgtaatg ttttgcatga tggtcttctc catgaaagaa aaacactcta ctagggtaca
     1321 gctcacaggg tggtggggac catctcat
//
LOCUS       DAZGBC010000427         1346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGBC010000427 DAZGBC010000000
VERSION     DAZGBC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.429"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..286
                     /gene="ycgZ"
                     /locus_tag="ACHK1J_005339"
     CDS             50..286
                     /gene="ycgZ"
                     /locus_tag="ACHK1J_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO7914451.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            329..601
                     /locus_tag="ACHK1J_005340"
     CDS             329..601
                     /locus_tag="ACHK1J_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO7914452.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSVQTLIKQLLVMKSAE
                     ENAFRQDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFNGKKINRIH"
     gene            630..896
                     /gene="ariR"
                     /locus_tag="ACHK1J_005341"
                     /gene_synonym="ymgB"
     CDS             630..896
                     /gene="ariR"
                     /locus_tag="ACHK1J_005341"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO7914453.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            1009..1257
                     /gene="ymgC"
                     /locus_tag="ACHK1J_005342"
     CDS             1009..1257
                     /gene="ymgC"
                     /locus_tag="ACHK1J_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO7914454.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          448 a          215 c          272 g          411 t
ORIGIN      
        1 tattgttacc tcacttaaca ttgattaaca tttttaacag aggcgtagca tgcatcaaaa
       61 ttcagtgact ttagattctg cgggagcaat cactcgttac tttgcaaaag ctaacttgcc
      121 tactcagcag gaaactctcg gggaaattgt gactgagatt ttgaaagatg gacgtaatct
      181 gagtcgaaag tcgctttgtg ccaaacttct ttgccgactg gaacaagcga caggggaaga
      241 ggaacagaaa cattataatg cactaattgg gttgcttttt gaataatgca acagcctgat
      301 tatttaacag gttagctatg aagtcgttat gaagacatct gataatgaaa gtataaaata
      361 tgaaattact ggccaggcgg tgctccagat actgcgcatg aagataaatt tttcggtgca
      421 gactcttatt aagcaattac tcgtaatgaa atcggctgag gaaaatgctt tccgacaaga
      481 tttaattgac agcataattc gtgattttag caacagtgat acaggagggc caaaccgaag
      541 aacagcgata gctgacaata aaagtatgtt caatggtaag aaaataaaca gaatacacta
      601 aaatttcata agtaagatga gaggttacca tgcttgaaga tactacaatt cataatgcaa
      661 taactgataa agcgttatca agttactttc gcagttcggg taatttgtta gaagaagagt
      721 cagcagtgtt agggcaggct gtgactaatt taatgctctc aggcgataat gttaataata
      781 aaaatattat cttaagtctg atacactccc tggaaacaac aagtgatatt ctcaaagctg
      841 atgtgatcag aaaaacactg gaaatcgtgt tgcgatacac agcggatgat atgtaacttt
      901 taattattaa gtataagttt atagaaaact cattcatctt atttttgtct gtcgctttag
      961 actatacaca gcataatttt attgggttaa tatttctacg aggctgacat gaataattca
     1021 atcccagaga gatttatttt tcaatgtgct ttgtttaaaa atctcgagag ggaggtgttc
     1081 atgacgcatg ggtatgttga tagtcatatt attgatcagg ctttacgctt gcgtttaaaa
     1141 gatgaaacca gtgtaattct ttctgatctc tatctgcaaa tattgcagta cattgaaatg
     1201 cataagacta cactaacgga tatcattatt aatgacaggg aatccgtgct ctcttagaac
     1261 ttgagtaagg ttttagaata aacatcagct gtatcaccat gctgatgcaa agtgagcatt
     1321 tcaggcgtta tgctttctta atatgt
//
LOCUS       DAZGBC010000428         1338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGBC010000428 DAZGBC010000000
VERSION     DAZGBC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.430"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..245
                     /locus_tag="ACHK1J_005343"
     CDS             <1..245
                     /locus_tag="ACHK1J_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7914455.1"
                     /translation="GSEESISLTFRNMNDFTPEQIARQIPRLKAMLAMRSLLRDLKAN
                     LLDNATFRKELEKILRDPELSQALRDELSALVPEQV"
     gene            266..>1338
                     /gene="tssC"
                     /locus_tag="ACHK1J_005344"
     CDS             266..>1338
                     /gene="tssC"
                     /locus_tag="ACHK1J_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7914456.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPET"
BASE COUNT          316 a          302 c          388 g          332 t
ORIGIN      
        1 atggctctga agaaagcatc agcctgactt tccgcaatat gaacgacttc acgccagagc
       61 agatcgcccg acaaattccc cgtcttaaag ccatgctggc catgcgcagc cttctgcggg
      121 atctgaaggc gaatctgctg gataacgcca ctttccgtaa agaactggaa aaaattctgc
      181 gggatcccga actgagtcag gccttacgtg acgaactgag tgcactggtg ccggaacagg
      241 tgtgattaac ggattaagag agagcatgct gatgtctgta caaaaagaaa ataatgtggc
      301 gggaaatgcg gtttctgaaa cgcattccgg cgacagcgta tatgcttccc tgtttgaaaa
      361 aattaatctg aatccggtat cagccctgag tgcgctggat atctggcagg atccgcaggc
      421 catgtcagag gcatcagcag atgaacgtct gaccgcaggg atgcaggtgt ttatggaatg
      481 cctggcgaaa gcgggagcac cggtggagaa actcgataag gcgctgattg accatcatat
      541 tgcagagctt gattatcaga tcagccgtca gctggatgcc gtgcttcatc atccggaatt
      601 tcagaaagtg gaatccctgt ggcggggcgt gaagtctctg gtggataaaa cggacttccg
      661 ccgtaatgtg aaaattgagc tgctggatct gtccaaagac gatctgcgcc aggattttga
      721 ggatgcgccg gaaattattc agagtggtct ttatctgcag acttatgtgg cggagtacga
      781 tacgccgggt ggtgagccca ttgcagccct gatttctgcc tgggagtttg atgcgtctgc
      841 gcaggatgtg gcgctgctga aaaatatttc ccgggtggct gcgtccgcgc atatgccatt
      901 tattggttca gttggcccgg ctttcttccg gaaggatacg atggaagagg tggccgccat
      961 taaggatatt ggcaatcact ttgagcgtgc cgagtacatc aagtggaacg ctttccgtga
     1021 aacggatgat gcccgctata ttggtctggt gatgccacgt gttctgggac gcctgccgta
     1081 tggtcctgat accgtacctg tccgcagctt taactatgtt gaagaggtga aaggtccgga
     1141 tcatcataag tatctctgga caaatgcgtc ttttgctttt gcggccaata tggtgcgcag
     1201 ttttgtcacc aacggctggt gtgttcagat ccgcggtcct caggccggtg gtgcggtgca
     1261 ggatttgccg atccatctgt atgatttggg aaccggtaat caggtcaaaa ttccgtcaga
     1321 ggtgatgatt ccggaaac
//
LOCUS       DAZGBC010000429         1329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGBC010000429 DAZGBC010000000
VERSION     DAZGBC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.431"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..247
                     /locus_tag="ACHK1J_005345"
                     /pseudo
     CDS             54..247
                     /locus_tag="ACHK1J_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            289..618
                     /locus_tag="ACHK1J_005346"
     CDS             289..618
                     /locus_tag="ACHK1J_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7914457.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            599..880
                     /locus_tag="ACHK1J_005347"
     CDS             599..880
                     /locus_tag="ACHK1J_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7914458.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            1063..>1329
                     /locus_tag="ACHK1J_005348"
     CDS             1063..>1329
                     /locus_tag="ACHK1J_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO7914459.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNP"
BASE COUNT          381 a          311 c          332 g          305 t
ORIGIN      
        1 acttcgggta aaccctcgtc gggaccactc cgaccgcgca cataagctct atcatagctg
       61 acagcgggta tggctttgca gggtggggat gggtaaggtg aaatctatca atcagtaccg
      121 gcttacgccg ggcttcggcg gttttactcc ggtatcacat gtaaccacag cgtgccgcct
      181 tacatgcgct gacgcgcata aacaacaagt tgctacatta gcttacaggt taattcacct
      241 tgcataagat tagcatatag gttaatgttt atcgaaatgg gaggtcatat gtttagcgtt
      301 gtttatcacc cggaagccag ggaagaagcc acagcattac ccataaaaat cagggtgaag
      361 tttgaccgtc tcatcagcaa actggaatat gacgcccgat tactgcggga accggataca
      421 aagcccctgg gggatggact ttttgagatc cgcacgatgg ggacggacat agccagaggc
      481 atctgggtat accacaaagg caataccatc atcatgctcc gggttttcat caagaagtct
      541 cagaaaacgc cagcaaaaga aatcgatctc gccaaaaagc ggttagcgga ggtacttaat
      601 gaaactggta agccataaag aactgcataa cgagtggatg caggacgacg aatatcgcgc
      661 tgcatggcag gaagaagaac gtaaggagaa actcagggag ttgcttgcag aatggcgtaa
      721 gcatgacaat ttgacaaaag cgcaggttgc tgaacgcatg ggggttactc ctcctgttat
      781 ttcccgcctg gaaaacaaca tcactaaagc cagcatcgac accctgactc gctatgccca
      841 tgcgtgcggt atcaaaaaac ctgttattac gctgtactga actctgctct atggctgcca
      901 gttctggaga aatcagtaac taaattttaa ttagctacgt tatggtaaat ctgcctgatg
      961 tgtcgatttc tctgaaagta attcggcttc tgtgggctgc gtttgccttg tgccacctga
     1021 atgtaatcac tatgattaaa tatgattaac aactaatcgg atatacaaat gaaaaacaat
     1081 accgcacagg caacaaaagt aattaccgcg catgtgccat tacctatggc tgataaagtc
     1141 gaccagatgg ccgccagact ggaacgctcc cggggctggg ttatcaaaca ggcactttct
     1201 gcatggcttg cccaggagga ggagcgtaat cgcctgacgc tggaagccct ggacgatgtg
     1261 atatccggac agattatcga ccatcaggct gtacaggcct gggcagacag cctcagtact
     1321 gacaatccg
//
LOCUS       DAZGBC010000430         1324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGBC010000430 DAZGBC010000000
VERSION     DAZGBC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.432"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..475
                     /gene="tssE"
                     /locus_tag="ACHK1J_005349"
     CDS             20..475
                     /gene="tssE"
                     /locus_tag="ACHK1J_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7914460.1"
                     /translation="MNRKSPSLYEILYGNFTGGLELNQVSEEEQVILSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTSILQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            528..833
                     /locus_tag="ACHK1J_005350"
     CDS             528..833
                     /locus_tag="ACHK1J_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914461.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            888..>1324
                     /locus_tag="ACHK1J_005351"
     CDS             888..>1324
                     /locus_tag="ACHK1J_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFO7914462.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILA"
BASE COUNT          332 a          308 c          341 g          343 t
ORIGIN      
        1 tacaaattgg attattatta tgaaccgaaa atccccatca ttatatgaaa ttctctatgg
       61 taatttcact ggcgggctgg agttaaatca ggtcagtgag gaagagcagg ttattctttc
      121 ggtgctcgat aacatgcagc gtattctgaa tacccgggct ggaagcctta aacatcttcc
      181 ggattatggt ctgccggata tgacaagcat tctgcaggga atgccgggaa cggcgcatca
      241 actgatgcgt gtgctttcag atgttctgct gaagtatgag ccacgtatta agcgtgtcaa
      301 cgtgacgatg caggagcaga ctcagcctgg tgagttgcat tatgtcattg atgcagaact
      361 taaagatgca ggactggttc gttacggaac aacttttatg ccggagggca gggtgttact
      421 gcgatatctg aagcaacagc agtttgtgga taatgtgatg actttcaggg ggtgatttat
      481 ggttcccctt atccgtgtgc ttaacagaca taaacgaggg ctgaatgatg aacccgttat
      541 ttaatgacat ccagatgcgt cttttttatc tgaaccactc tccgtactcc tggcactgga
      601 atgtcagatt cagaccccag gaagctgtct atattggcag cgatacctgt catatcacca
      661 ttacctgtaa tcagtcaggt tttcatttaa cccgggacgg acaacggctg ttcactgagc
      721 gttatatcag aaaccttaat gagctgttac cggttctgaa acggcggtgg gatgtgactc
      781 cggcaattat ccgcgcggtg gaataccttt cccgggtacc ggtatcacac tgatgtcctt
      841 tccgtaactg ttaatcatga tttaatacag caacaccgaa tctgccgatg acttctgaag
      901 tgaaactgaa aaccggtggt gacccccggt cattaccgga ttatgctgcc ctgcgtgatg
      961 aaatcagcaa actgacccac ccggcacgtc cggatgtgga ctggcgttac gtggaaacgc
     1021 tctgcctgcg tctgtatgaa cacaatggcg tggaattaca gacggtatcc tggtacacca
     1081 ttgcccggat gcatacagca gggttaagtg gtctgaatga agggctggct ctgattgtgg
     1141 cgctgacccg gcatcactgg tcagtgatgt ggccgctgaa tacccacgcc cggctggaaa
     1201 ttattaccgg tctgttcagt cgtctgcaaa aaacgctccg ggccatgcca ccggatgagc
     1261 gtgacaacct gcccctgctg tatcagacag aaacatgcct gaaaacgctg agtgacatac
     1321 tggc
//
LOCUS       DAZGBC010000431         1305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGBC010000431 DAZGBC010000000
VERSION     DAZGBC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.433"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..249
                     /locus_tag="ACHK1J_005352"
     CDS             <1..249
                     /locus_tag="ACHK1J_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO7914463.1"
                     /translation="EMLQDKNEEVRIDAIIGLSYRKDKRVLSVLCDELKKNTVYDDII
                     EAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            277..1198
                     /locus_tag="ACHK1J_005353"
                     /pseudo
     CDS             277..1198
                     /locus_tag="ACHK1J_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1198..>1305
                     /locus_tag="ACHK1J_005354"
     CDS             1198..>1305
                     /locus_tag="ACHK1J_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914464.1"
                     /translation="MKYIIFSFRAIWLALSLLMLFFSMHRLSLLDSTRDV"
BASE COUNT          374 a          251 c          341 g          339 t
ORIGIN      
        1 gagatgcttc aggataaaaa tgaagaagtc cgtattgacg caataatcgg actttcctac
       61 agaaaagata aaagggtttt atctgtttta tgcgatgagt taaaaaaaaa tactgtttat
      121 gatgatatca ttgaagctgc gggtgaatta ggtgataaaa cgctacttcc tgttttagat
      181 actatgttgt acaagtttga tgacaatgaa attataactt ccgctattga taagctgaag
      241 cgttcatagc gtgagttgcc tatgcacagt gggggattcc cgccggcacg gtgcagatgc
      301 tcgaccggct ggaaagtgaa atcctggctg agcgggtgag tgaggaaagc cgccgctggc
      361 tggcatcgtg cggcctgacg gtggagcaga tgcaaaacca gatggacccg gtgtacacgc
      421 cggcgcgaaa aatccacctg taccactgcg accatcgcgg cctgccgctg gcgcttatca
      481 gtgcggaagg ggctacagaa tggtgcgcag aatacgatga atggggcaac ctgctgaatg
      541 aagagaaccc gcaccagctg cagcagctta tccgcctgcc ggggcagcag tatgatgagg
      601 agtccggcct gtattacaac cgccaccgct attatgaccc gctgcagggg cggtatatca
      661 ctcaggatcc gattgggctg aaggggggat ggaattttta tcagtatccg ttgaatccga
      721 tctcaaatat agatccatta ggattagaaa cactaaaatg cattaaacca ctgcattcaa
      781 tgggcggaac tggtgaaaga agcggtccag atatatgggg gaatccgttc tatcatcaat
      841 atctttgtgt cccagatggt aaaggggact atacttgtgg tggccaagac caacggggag
      901 aatcaaaagg agatggtcta tgggggccag gtaaagcaag taatgataca aaagaagctg
      961 ctggccgttg tgacctcgtt gaaaccgata atagttgtgt ggagaactgt ttaaaaggga
     1021 agtttaaaga ggtaaggcgc gttattctgt attgcctgat atattcacac ctataaattt
     1081 agggctattt aaaaactgcc aagactggtc taatgattct ttagaaacat gtaagatgaa
     1141 gtgctccgga aataacattg gacgctttat tagatttgta ttcaccggag tgatgtaatg
     1201 aaatatatta ttttttcatt cagagctata tggctggcgt tatcactttt aatgctattc
     1261 ttttccatgc atagactttc actattagac tccactcgtg atgtg
//
LOCUS       DAZGBC010000432         1294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGBC010000432 DAZGBC010000000
VERSION     DAZGBC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.434"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHK1J_005355"
     CDS             <1..315
                     /locus_tag="ACHK1J_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO7914465.1"
                     /translation="RTQSRRLKLSTRRARAAQFRDGWVQGIRQVVMPFSVTEEESRLM
                     RLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNASLHHGVGESARQVVPGLMIRE
                     AS"
     gene            312..920
                     /locus_tag="ACHK1J_005356"
     CDS             312..920
                     /locus_tag="ACHK1J_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO7914466.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            935..>1294
                     /locus_tag="ACHK1J_005357"
     CDS             935..>1294
                     /locus_tag="ACHK1J_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO7914467.1"
                     /translation="MAEINFQVLRENDAGELTGVATAGRPVARHFDDGLTLEVNRTCT
                     TGERNANSALYGAVWRAAKDMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAE
                     SSVALKSTRDLVGSGGVP"
BASE COUNT          293 a          289 c          438 g          274 t
ORIGIN      
        1 cgtacacaga gccggagact gaaactgagc acccgtcgtg cccgggcggc tcagtttcgt
       61 gatggctggg ttcaggggat tcgtcaggtg gtgatgccat tcagtgtgac agaagaggaa
      121 agccggctga tgcgtctctg gatggagcag caggatatgg tgacggtgga ctcccgtgag
      181 atgaaagcct gtcgcggcgg tgacctggcc cgctggcagg ggtatgaggc cgggaaaaat
      241 gcctcactgc atcatggtgt gggggagagt gcccgacagg tggtgccggg tctgatgata
      301 cgggaggcgt catgaataac agcttgtctc aggcaaccaa cggtctgctg atgcaactgg
      361 tgatggacct caagagcggc tacctgcgtc gctgcgaatc tctggggctg gcccgggagg
      421 aaatgcagat gctgcaggga ctgaccattg aggaaattca ttacctgtca aactctgaag
      481 tgtcggttat ccggctggac attaatcatg aaaacctgct gcgtatgctg cagcaggccc
      541 ggacggagca gaaacgtctg cagcgcattg accgggcact ggcgctggga ggctccattg
      601 aactgatggc gttttacttt ggactttcca gtgtggatgt ggcggcgcgc cgtcgcattg
      661 ccggtattga tgttcgcccg ggacgtggca tcacactgag tgatgaggaa agtgcagagc
      721 tgtggcgttt atggcagaag agcggcatca cggatgtgga gagtgcggac ggactggatg
      781 tcatgatgct ggcagctgag caaatgaata tcccgctgac ggcggtatgg catgccgtga
      841 ggggatggca tcagggaact gcggggttta atgatgtatc ggccagtcgc cggaaactga
      901 accgggaaaa agcagaatga caaggacaaa tgacatggcc gaaattaact ttcaggtact
      961 gcgggaaaat gatgcgggtg aattgacagg agtggcgaca gctggcagac ctgttgcccg
     1021 ccactttgac gatgggctga cactggaggt caatcgtacc tgtactactg gtgaacgtaa
     1081 cgctaacagc gcactttatg gcgctgtctg gagggcggca aaagatatgg gctatcgccg
     1141 ttgcattaca tacaccaggg ctgatgaatc cggagcctct cttcgggcag ctggttttgt
     1201 tcgtgtgaaa gaacttcctt caggaaaaag ctgggcggaa tcaagtgtcg ccctgaagag
     1261 tacacgcgat ctggtcggaa gtggtggtgt tcct
//
LOCUS       DAZGBC010000433         1294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGBC010000433 DAZGBC010000000
VERSION     DAZGBC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.435"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..589)
                     /locus_tag="ACHK1J_005358"
     CDS             complement(113..589)
                     /locus_tag="ACHK1J_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7914468.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(605..1090)
                     /locus_tag="ACHK1J_005359"
     CDS             complement(605..1090)
                     /locus_tag="ACHK1J_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7914469.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(1181..>1294)
                     /locus_tag="ACHK1J_005360"
     CDS             complement(1181..>1294)
                     /locus_tag="ACHK1J_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFO7914470.1"
                     /translation="SAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT          297 a          322 c          379 g          296 t
ORIGIN      
        1 tgttggtgaa tacgcatggc ttcaccacgg gtgatgattt tcatgatgtg atacctctga
       61 agacagaaga taaaagtgaa aacaggtatg atgtggttgt gacggtgacg ggttaaagca
      121 gaccgtgttc ggcaaaggag aaaacctggc tgccaccgac tatcagatgg tccggcaccc
      181 ggatatccac caggcccagt gcctgtacca gacgttccgt aataagccgg tctgccttac
      241 tgggtgtgac ttcaccggac gggtgattgt gtgccagtac cacggcagcg gcattgtggt
      301 acagggcgcg tttaatcact tcccggggat ggacttccgt gcggttgatg gtgccggtga
      361 agagggtttc accggcaatc aactgattct ggttgttcag atacagcacc cggaattctt
      421 cacgctccag tcccgccatg ttcagaatca gccattcccg tgccgctcgg gtggaggtga
      481 aggccacgcc gggttcatga agatggcggt ccagggtttt cagggcccgc agaatgagac
      541 tgcgctcgcc gggcgtcatc tctccgggca gaaaggaaag ttgttgcatt gtgcttctct
      601 ccattcagtc gatgatgcgc ataatggcgc tgcattccgg atgctgcagg gcgtaatccc
      661 gcaaccggta ataatggacc gtcatggcat aacactccgt acgacaggca tgatgactgt
      721 acgtcatcag acaggcagca atgccggcgg cttccgggct catttcagcg cggttaccgt
      781 tcatggcatt gaacagtacc cagttttcgc catcatcgtc atccggttcg ggggccataa
      841 atgcgccacc gttgttcagt gtgtacagat tccagatacc accgcagtag tcttcgcaaa
      901 gacggtccat ccagccgaag atacggggct ccagggtcac ccactgtgga atgaggccaa
      961 aatgctgcgg ccagaagctg atgcgctgtt catcagggac tatggtggca accagctgag
     1021 gctggtcact ccctgatgca gttgttacgg aaacagaagg tgtggtggag ctatgcgaaa
     1081 aggttgtcat gaaatgattc cttataaaaa gcaaatgaat gaagaagact cgggggcagg
     1141 gacagacgtg agtcaggacg gcgctttcag gtaaacggta tcagcgcatg ctctccagca
     1201 gcgtttccgc catcacccac aatgcgcgat tgagcttgat gtcggtgtcg atactgtgaa
     1261 tggcacgggt atggatacgt tttccttttg cact
//
LOCUS       DAZGBC010000434         1292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGBC010000434 DAZGBC010000000
VERSION     DAZGBC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.436"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..683)
                     /gene="tssI"
                     /locus_tag="ACHK1J_005361"
     CDS             complement(<1..683)
                     /gene="tssI"
                     /locus_tag="ACHK1J_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO7914471.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTCTEVSTLCISQFRYSA
                     QIRPSSVVT"
BASE COUNT          371 a          309 c          303 g          309 t
ORIGIN      
        1 ttggtcacca cggaagaagg gcggatttgt gcgctgtagc ggaactggct gatgcagagg
       61 gtgcttacct cggtacaggt gttcggattc caggggatct caaaggactc cggcagataa
      121 cgcacggtgt cgcacaggac caggctctgg tcggtacttt tttgcgcgtg ctcctcataa
      181 aagaagatgc cttcctccgc cgccatccgg cacaggaaat cgtaatcagt ctcaccgtac
      241 tggacacaaa actcacggga aggatgcggt tcactgaaca gcggactcca ctcggtcacc
      301 ccgttttcct gcaatatcgt gccgaggatg ctttcgatgt cctcattctg gaagatacgg
      361 aagttctggc gcagccctgt gcgccacagc ggtgggcaca ccttcatgct gtacagcatc
      421 tggtttttgt cgttctcccc cagttcaaac caggtcacca cacctttcac ccggcgctgt
      481 acgtcatcgc cctgccatac cgtcaggtag gccattttgt ccagcacctg cgcaaattca
      541 agggaaagaa actgctgact gaccagagaa aggtcgaggg aaaaaagtga agagagtgac
      601 tgggtgagat gaaaggaaac caccgcaaaa gcatccggtg gcaggccgtc cacttccagc
      661 gtgaaacgta atccggttga catagcaacc tccgtgaaaa tgaataataa tccgcacata
      721 ttgcatgtac aacatgtcgg gcggatgaga taagcgagtc gacatctggc gttaccgggg
      781 aatatcagaa aaatgtcatg gttatgtgat gatttagtct gggattttac ttcagagaaa
      841 cgggtgtctg tttagtcaac aaaataatca caaacgcaac ggatgggttc tcgacgttat
      901 tcacgacagg ctcgtacatt acagcgagta ataaaaaacc tgatatgcaa agtcaaacca
      961 atattcataa tgtcttttta ttcacaacaa taaataattt aataacaatc attgaaatga
     1021 atgatgattt gtataaggaa tatacgctac cgccccaccc tgataacatg atctgcacat
     1081 tgccatctta acgtcgttaa acactggctt aatagtggat atttcaaatt aggaatttat
     1141 taaattcacc tgatgaatat cataagcaaa tagcatagaa aataatgaca actcccccga
     1201 ggggaccatt tccaaagcac tgcaccacca cctggtgccc ggatgatctt gcttcagtgg
     1261 taagagtacc tgatgcattg ttcccaaaat gg
//
LOCUS       DAZGBC010000435         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGBC010000435 DAZGBC010000000
VERSION     DAZGBC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.437"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1005
                     /locus_tag="ACHK1J_005362"
     CDS             <1..1005
                     /locus_tag="ACHK1J_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO7914472.1"
                     /translation="DSQVAYNATVVSGTMSSNGSSGGSASDAISGDASNTQTTTVDMK
                     SALYQDVKNAVTSMLTPGIGRMFMSTGFITVTDNPQVLETVRAFIEKRNEEMKRQVVL
                     NVEILSIRKTRNEQAGIDWNAVFSDRTLGLSLGSTFTSAASDAITGGVSIVDGKLTGS
                     KAFLKALSSQGDVSVVTQNSAVTKNLTPVPMQIANQQSYIESVTTDTTANVGSSTSLN
                     AATITTGFNMTLLPFILPDSQTLQLLYSMSLSDKPVIENYESGGSKAQLPNVDLKTIN
                     QTVDLKSGQTVIISGFQQSGRRSGKQGVGTPGFFGLGGGINSENDDTILVVLITPNII
                     "
     gene            1026..>1291
                     /locus_tag="ACHK1J_005363"
     CDS             1026..>1291
                     /locus_tag="ACHK1J_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO7914473.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLIL"
BASE COUNT          320 a          359 c          338 g          274 t
ORIGIN      
        1 gacagtcagg tggcctataa cgccactgtg gtcagcggca ccatgtcctc aaacggctca
       61 tcaggaggca gtgcatcaga tgccatttcc ggtgatgcca gcaataccca gaccaccacc
      121 gtggatatga agtctgcgct gtatcaggat gtgaaaaatg cggtcacatc aatgctgacc
      181 ccgggtatcg gacgcatgtt catgtccacg ggctttatca ccgtcacaga taatccacag
      241 gttctggaaa cggtccgggc attcattgaa aaacgcaatg aagagatgaa acgtcaggtg
      301 gtgctgaatg tggaaattct gagtatccgg aaaacccgca atgagcaggc cggtatcgac
      361 tggaacgcag tcttcagcga cagaacgctg ggactgagtc tcggcagcac attcaccagt
      421 gcagccagtg atgccatcac aggtggtgtt tccattgtgg acggtaaact caccggttca
      481 aaagccttcc tgaaagcatt gtcttcccag ggggatgtca gtgtggtgac ccagaactct
      541 gccgtcacca aaaacctgac gccggtgcca atgcagatcg ccaatcagca aagttacatc
      601 gagagtgtga cgacggatac cacagccaat gtcggctcat ccacctccct gaatgcggcc
      661 accatcacca ccggttttaa catgaccctt ctgccgttca ttctcccgga ctcacagaca
      721 ctgcagttgc tctattccat gagtctctcc gacaaaccgg ttattgagaa ttatgagagc
      781 ggtggaagta aagcccagct gccgaatgtg gatctgaaaa ccatcaatca gaccgtggat
      841 ctgaaatccg ggcagacggt cattatcagt ggcttccagc agtcaggacg tcgcagcgga
      901 aaacagggtg taggtacgcc gggattcttc ggtcttggcg gtgggatcaa cagtgaaaac
      961 gacgacacca ttctcgtggt gctgatcacg cccaatatca tctgatttgc acaaggagga
     1021 cgatcgtgac ggatatttca ccgacagggc ggcaggtgtt tccgctgacg gcagggcgca
     1081 atgtgtttct ggccggtctg gaatggaaaa cactgccctc acagtaccgg catgcccgcg
     1141 actttgcccg ggcacagaaa gccgaccttt tcctggcctg tcagtatctc agtaatgaag
     1201 atgccgatac ccacaccatg gtagccactg tcagtcgccg catactgccc ggaaaacccc
     1261 gtcagtgctt ctccctcgcc ctgctgatcc t
//
LOCUS       DAZGBC010000436         1289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGBC010000436 DAZGBC010000000
VERSION     DAZGBC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.438"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..70
                     /locus_tag="ACHK1J_005364"
     CDS             <1..70
                     /locus_tag="ACHK1J_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914474.1"
                     /translation="VEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(106..1248)
                     /locus_tag="ACHK1J_005365"
     CDS             complement(106..1248)
                     /locus_tag="ACHK1J_005365"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO7914475.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIYRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSTFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          406 a          254 c          213 g          416 t
ORIGIN      
        1 cgtagaacaa attgaaccaa atttattagg cgtttacgcc ggcttattat tagcgatgaa
       61 aaataagtaa aaaataaaaa aggctgcata atgcggcctt tttatttatt caaaccaatt
      121 atgaataacc tcttcgaatc tttgagttac gccatcccaa ctgtagtgat caaacacaaa
      181 atcctgacct tgctttgcaa ctgctgttaa ttcaggattc gctaacgttt ttaaaatatc
      241 gctgcttatg gagtctgcag tcataggttc ttttaaatgg aatcccgtag tattttcttt
      301 gacaaattcc gtcatccccc cacgcgtact cactaaaaca ggcttaccag cacccatagc
      361 ttcaattgcc accatgcaaa atggttcctg gaactgggaa ggaatgacga ctaaatctgc
      421 cagtgggtaa taacaatgca tcttttcagg cggtatgctt cctaacataa tgcaacgatc
      481 cttcaaacgt tttgcaattt ccctgacatt gcgttgatat gcacctttgt cacttttact
      541 catttctgta taatcaccaa tcacaactaa ttttaaattt ttgtgagcag cggccaattt
      601 ttcgaatgat tgcatcaaca gcaatatgcc tttgtcaggg acgatacgcc cagcataaaa
      661 aataattttc tcttcagggg agatattaag ttcgcttcta gtgatcggct gaaaagtaga
      721 ttgataggtt tctaaatcaa ttccatttgg tacaatctca atatctgcat ttgctaaata
      781 agattggtaa tactttttca agtataaact aggcactatc attttaacat tttgctcaag
      841 caattttggt tcaaatgcat tatgcatatg tataaccact cttgcctgag gagctcgctt
      901 acgaatttga gtgtatagct tcatactatt atgaactaca ataacacttt ctttcgttat
      961 aggaaaatca tgtgcaatat tcaatattct ctgtgaatat ggaagaggat caagtcttgt
     1021 ccatttttga aataaccgct tatatatgcg actgaatcca accctataaa tactacaatt
     1081 atcattaaca aaggtgtaat ttgagtatcc tggattcttg atacaaacaa tacgatttgg
     1141 gaaattggta cgctgagcaa cctgatacat ccacgtttca acagcggccg ctccgcgagg
     1201 aggaattgaa aaaataggag taactgtaaa tattatttta tcaaccatct cgaatgttaa
     1261 cctttcataa aattatattt aattgctgt
//
LOCUS       DAZGBC010000437         1284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGBC010000437 DAZGBC010000000
VERSION     DAZGBC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.439"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1284
                     /locus_tag="ACHK1J_005366"
     CDS             <1..>1284
                     /locus_tag="ACHK1J_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7914476.1"
                     /translation="KAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTITYE
                     DLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLKLI
                     LNHGIDVITLCDNTVYNIDSLNDPYSLIKAILIAQRANEESEIKSSRVKLSWKKKRQD
                     ALESGTIMTASCPRWLSLDDKRTAFIPDPDRVKTIELIFKLRMERRSLNAIAKYLNDH
                     AVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDLFY
                     AVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMRRL
                     HRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLIAA
                     LSVAPEVTAIAEKIRVLDKELRRASVSLKTLKSKTV"
BASE COUNT          440 a          187 c          281 g          376 t
ORIGIN      
        1 aaaagccata gcatatatgc gattttcatc accaggtcag atgtctggcg actcattaaa
       61 ccgacagaga agacttattg ctgaatggtt aaaggtaaat agtgattatt atcttgatac
      121 cataacatat gaagatttag gattaagtgc attcaaagga aagcatgcac aatcaggagc
      181 tttttcggaa tttttagatg ctatagagca tggttatata ttgccaggaa ctacattgtt
      241 agttgaaagt ctggacagac tttcaagaga aaaagtcggt gaagcgattg agcgtctgaa
      301 attgattttg aatcacggta ttgatgttat aactctttgc gataatacag tctataatat
      361 tgactctttg aatgatccat attcattaat aaaagccata cttatagcac aaagggcaaa
      421 tgaagaaagc gagataaagt caagtcgggt taaattatca tggaagaaaa aacggcagga
      481 tgcactggag tcaggcacga ttatgacggc gtcttgtccg agatggctct cattggatga
      541 caaaagaaca gcttttattc cagaccccga cagggtgaaa acgattgagc taatttttaa
      601 actcaggatg gaaaggcgat cattgaatgc aatagccaag tatttaaatg atcatgctgt
      661 aaaaaatttc tcaggaaaag aaagtgcatg ggggccttct gtaattgaaa aattattagc
      721 gaataaagct ctgataggta tatgcgtacc ttcatatcgt gcaagaggta aaggaataag
      781 tgaaatcgct ggctattatc ccagagtcat atcagatgat ttgttttacg ctgtgcagga
      841 aattcggttg gcaccttttg gtattagcaa tagtagcaaa aatcctatgt tgataaatct
      901 acttcgaaca gttatgaagt gcgaggcttg tggtaatacc atgattgttc atgcggtatc
      961 tggaagtttg catggctatt atgtttgtcc gatgagaaga ctgcatcgat gtgacaggcc
     1021 atcaataaag agagatttgg ttgattataa tatcattaat gagttgcttt ttaattgtag
     1081 taaaatccaa ccagttgaaa acaagaaaga tgctaatgaa actttagagt tgaaaattat
     1141 tgagctccag atgaaaatta ataatttaat tgctgcatta tctgttgcgc ctgaagttac
     1201 cgctatagca gaaaaaatca gagtattaga taaggaatta cgaagggctt ctgtatcatt
     1261 aaaaactttg aagagtaaaa cggt
//
LOCUS       DAZGBC010000438         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGBC010000438 DAZGBC010000000
VERSION     DAZGBC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.442"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..1248
                     /locus_tag="ACHK1J_005367"
     CDS             106..1248
                     /locus_tag="ACHK1J_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO7914477.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          393 a          271 c          278 g          335 t
ORIGIN      
        1 ttaaagggat tataaataaa gccagaactc ttggttccaa agctggcaac aactaaccag
       61 ttaaaacata cgcatcaaaa tcagataaac atttgaggta ttgttatgac aaataaacac
      121 ccatcattgt tttctccttt tatgctgaca gaaaaaataa agctacgtaa tcgtatcgtt
      181 atggctccta tgacgacctg gtcagcgaat cctgatggaa ccatctctga gcaagaactc
      241 gaattttata aacgtcgctc acaaaatgtt ggtttagtaa taacaggatg tacctatgtc
      301 acgccaagcg gcattgggtt tactcatgaa tttgccgcgt atgacgaccg tttcattaac
      361 agccttgaaa agttagcagc agcagccaaa agtgggggtg ctccggctat tttgcagatt
      421 ttccacgccg gtaataaagc catcccggag cttgtaccaa ataacgatgt tattagtgcc
      481 agcgcatcaa gcgtaaaatc tggtgacttt atgaagagag tggtacaaag cagggaaatg
      541 actgaaaacg aaattcagga aaccattaga gcattcggag atgtcacaaa aagggcgatt
      601 aaagcaggtt ttgatggcat tgaacttcat ggtgcgcacg gattccttct acagaatttc
      661 ttctcccctc tatttaacca gcgtaatgac cgatggggag gagatctcga aggacgtatg
      721 cgcttcccgc tggccgtatt acaggaagta aaaaatgtag tctatgaata tgcgacaaag
      781 ccctttgcca tcggttacag aatatctcct gaagaatctg caacaggggg gctgcggata
      841 gaggatactt ataaactcct tgatcggctc atttcatccg gtatttcgta tatacatacc
      901 tcgctggtca gtattaacga tagctaccct gttgagtcgc caaatggccc ccgaactatt
      961 gaattgattt taaatcatat tgcaggcaga gtgccggtaa tcgctgcagg taaaatacgc
     1021 acaccgtctc aggctcaaga agcaatttcc actggcctgc cacttgttgc gatcggtaaa
     1081 gggctggtga taaatccaga atgggtgact ttagctgaat caggtagagg ccatgaaatt
     1141 caaacaaccc ttaatccaca acttgttcct gaattaacca ttccggataa actatgggat
     1201 caaattcagg catcgaaagg aactggctgg tttccgttaa tggattaaat atctattact
     1261 ctcccgagag taaaact
//
LOCUS       DAZGBC010000439         1275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGBC010000439 DAZGBC010000000
VERSION     DAZGBC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.443"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..777
                     /gene="ais"
                     /locus_tag="ACHK1J_005368"
     CDS             175..777
                     /gene="ais"
                     /locus_tag="ACHK1J_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO7914478.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(816..1241)
                     /gene="nudI"
                     /locus_tag="ACHK1J_005369"
     CDS             complement(816..1241)
                     /gene="nudI"
                     /locus_tag="ACHK1J_005369"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO7914479.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEIKPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          347 a          283 c          266 g          379 t
ORIGIN      
        1 aatattgaaa tttttatatt ttttcttatt taggctttgc atttggcaaa attttgaggc
       61 attttgccga catcgtagga tttttaatat tacaccaact gctaattatc gccagaaatg
      121 tcgctcaatt tcacggtaat tgtctggttg cgcttgtcta taggtggagt ttacgtgtta
      181 gctttttgcc gctcatcatt gaagtcaaaa aaatatttca tcattttact ggcgctcgct
      241 gcaattgccg gactgggtac tcatgccgcc tggagtagca atggtttgcc acgtatcgac
      301 aataaaacac tggccagact ggcacagcag cacccggttg tcgttttgtt tcgccatgct
      361 gaacgttgcg atcgttcaac caatcaatgc ttgtcagata aaacaggtat tacggtcaaa
      421 ggtacccagg atgcccgaga actgggcaac gcttttagtg ctgatatccc tgatttcgat
      481 ctctattcca gtaataccgt ccggaccatt cagtcggcga cctggttttc agcgggtaaa
      541 aaattgacgg tagataaacg acttcttcag tgcggtaatg agatttatag tgcaattaag
      601 gacttacaaa gcaaagcgcc tgataaaaat atcgttattt tcacccataa tcattgcctg
      661 acatatattg ctaaagataa gcgtgatgcg acatttaaac ctgattatct ggatggttta
      721 gtcatgcatg tggaaaaagg caaagtttat ctggatgggg aattcgttaa ccactaaatt
      781 ttcaatctga cagccagtga tggctgtcat cattgttaaa gaagaccttt caaacgtaac
      841 gtttttcggg tggcaacatt caaatcataa tgcaccagat cttcaggttt tacccacgcg
      901 tagtcctgaa actcttcgtt tattttcact tctcggttgg cagaaacgca gtcaaaaatc
      961 aggtaaatca tataaatctc ttccttgcga ccatctgcat acgtcttggt gcgaatatca
     1021 tcgctgaagg tccacggctt gatttctgtc aaaagcagct gttctcccag ttcttcgcga
     1081 atttcgcggc gtagtgcctc ttcaattcgt tcgccgggct ccacgccgcc acccgaaagc
     1141 gcccattgac cggggaaaac gccgcgatcg tcggccattt tacacagcaa ataagcacca
     1201 tcattttgaa tcaaagggca tacaatagtc cgttgtcgca caggggtatt ccttataaat
     1261 aaatagaaac catga
//
LOCUS       DAZGBC010000440         1271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGBC010000440 DAZGBC010000000
VERSION     DAZGBC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.444"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(43..231)
                     /locus_tag="ACHK1J_005370"
                     /pseudo
     CDS             complement(43..231)
                     /locus_tag="ACHK1J_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            289..1120
                     /locus_tag="ACHK1J_005371"
                     /pseudo
     CDS             289..1120
                     /locus_tag="ACHK1J_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          353 a          290 c          288 g          340 t
ORIGIN      
        1 ccatttagct gtaagtgcat taatcgaatg ccgtattgac gcttatttat cggtgagaac
       61 tacgttccat ggcagcagtt cgtcaacacg gttggaaggc cattccggca gtacgctcag
      121 aatatggcgc agatacgctt ccggatcgat actgttcagt cggcaggtgc caatcagccc
      181 gtacagtagc gctccacgct ctccactgtg atcgttgccg aagaacacgt agatgatatg
      241 acctttatat actcactata gcattggtgg gatactccag aggtcatcat ggattcggaa
      301 aatattgcag cttacgtcgg gctggatgtt cataaagaaa ccttggcgat tgctacacct
      361 gaatggcttg gcgaagttcg ttattatggt acgattaaca acgaagcctt ggctgtacat
      421 cgattatgtc agaaattgca gggaccttac ggaaacattc tttcctgcta tgaaggtggt
      481 ccctgtgggt ttgggctata tcatcaactc acagtgatga atataaaatg cctggttatt
      541 gcccccttcc cgtataccca aatcaccggc agacaggatc aaaaatgacc atcgggacgt
      601 aatttctctc gccaggttac tgagggatgg tgaggttccc ccagtctggc tccctgagct
      661 gactcatgaa gctatgcggg atttaatcag agcccgtgcc gctgcaaaac gtgacacgaa
      721 cagacaaacg ttatgccagg aagcactgaa ccggcaaaca tcgaacctgg ttagttaatc
      781 aatcatttgg tctgctatct caacagattg ctttcaacat tattgtcaga atctggagca
      841 gattgaagat cgcatacttc aactcgatca ggaaatatct cgcctgttac ctgagtgatc
      901 tctgtgcaat ctggtctgcc agttgcaggc tcttaaaggt gttggtcagc taatcgcaat
      961 aaccctggtt gctgaactgg gtgatttttc gcgattttcc aatcctgaac agctcatggc
     1021 ttttctggga ctggtgccag gtgaatattc cagcggaaac agcattcgcc ccagaggaat
     1081 aacaaaagtt ggaaacagtg aactgagaca tctgctttac ggatatcaaa taaattcaga
     1141 ttatttaaca gtaaaacccc acttatgtga tgaatctcat aaaaaagcaa agtgaagtaa
     1201 taatatccaa tttatccctc attccttaag taagcataca ctggccgcgt acaccatggc
     1261 ataatgatat g
//
LOCUS       DAZGBC010000441         1266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGBC010000441 DAZGBC010000000
VERSION     DAZGBC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.445"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..>1266
                     /locus_tag="ACHK1J_005372"
     CDS             33..>1266
                     /locus_tag="ACHK1J_005372"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HFO7914480.1"
                     /translation="MLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLG
                     QRRIALPGYSTVQNLVSDALNFERKRTINILSKRIYPATAQKITSIIHDDGILNSIRG
                     YNYSTRDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFR
                     LKRHPKWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLI
                     NKKLKFASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEY
                     QWQYIDSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRV
                     IHPSEKNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYW
                     HKNKSILIEKTGLERLITP"
BASE COUNT          467 a          207 c          233 g          359 t
ORIGIN      
        1 ctaaaagtac aaagtaccgc cttgttaaac gaatgctttc tactctagga tttgaacaac
       61 tcaccccatc tataaaaaat gagttaagtg aacgtctgaa tgatgttgct actatatgta
      121 tggaaccaaa atatattttt gatgaattac ttgctttttt aggacagcgg cgtatagctc
      181 ttcctggata cagtactgtc cagaacctcg tttcagatgc tttaaacttt gaaaggaaaa
      241 ggacaattaa tattttgtca aagagaattt atcctgccac tgcacaaaag attacatcca
      301 tcatccatga tgatggaata ttaaattcca tcaggggata taattatagc acgagagact
      361 tctcagcatc agaaatagaa aatgaattaa gaactcacaa tgtgataagt agtatttact
      421 atgaaataaa agatataatt aattcattag gtatatccca gggaaacatg gagtactatg
      481 caactatcgt aaaacacagt acaacattca gacttaagcg acaccctaaa tggcaaggaa
      541 tactttattt gtgctgctac ttgtacttca gatacagaga gataaatgat aaaataatca
      601 cagccttcag atatttagta aaaaaacata aagacgcttc agttttgttt gcaaaagctc
      661 gggcaacaga ggaaatagat ctaataaata aaaagcttaa gtttgcgagt aaaatattta
      721 acttcttcgt tgatagtaac attgaagaca gcatcccttt tggatatgtt cgtgaaaagg
      781 ttttttcact tatatcgaaa gatgatatgc atcttgtgag tgaatttttt gacaaaaata
      841 aaatcgatgt aattgaatat caatggcagt atattgacag ccatcacctt aagatcgcca
      901 attcaatccg aaagcttttt ctggctatag atatagtata tgacaacgag aagtctgtta
      961 ttggccagca aattactgct gccaaggatg atctcagagg aaaaggaaag ctggatgcta
     1021 aggatctgag ggtaattcat ccatccgaga aaaattacat tgtaaaagaa ggagaaatta
     1081 acactcaacg attcgaattt catatgtaca gcagggtatc taaccatctg gaaaacggtg
     1141 tcttttatgc tgcggagagt gaacaaaaca agagactgga agatgatctc atagaacgaa
     1201 gttactggca taagaataaa tcgattttga tagagaaaac aggccttgaa cgacttatca
     1261 caccaa
//
LOCUS       DAZGBC010000442         1256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGBC010000442 DAZGBC010000000
VERSION     DAZGBC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.446"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1256)
                     /locus_tag="ACHK1J_005373"
     CDS             complement(<1..>1256)
                     /locus_tag="ACHK1J_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFO7914481.1"
                     /translation="RARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLG
                     YIRHSASLGASDLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMT
                     DVIKGTQFDPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPT
                     FSMLGYHPDQECTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGI
                     LLPRRRLFTIESPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDS
                     AITAIKAAMTGHLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLI
                     CPHCRHPWNDVASSRTKDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVIS
                     PDARFFQLYREKGRIEAKTYWHRELGG"
BASE COUNT          264 a          329 c          366 g          297 t
ORIGIN      
        1 ccacccagtt ccctgtgcca gtaggttttg gcttcgatac ggcctttttc ccggtataac
       61 tgaaaaaatc tggcgtcagg agagatgact tctgcaatca ctgtacgccc tgtgacacca
      121 cgccagcagt gtggacagcc ttcatggttt ctcagatata ccgcatcagc attacagaag
      181 gtttccacca gatgtcgctc atcctttgta cgactggatg ctacgtcatt ccatggatga
      241 cgacagtggg gacatatcag ctgaaccaga cgctgactga gcagtccgat gaacaactgc
      301 gggtctgcaa tcatcctggc ctgaacaccc tccatctcaa ggcgttcgag gatgttaatc
      361 gggtcgttcg catggatggt ggtcagcatc agatgcccgg tcatggcggc ttttattgcc
      421 gtaatggcgg aatcgtgatc gcggatttca ccgttcagga tgctgtcagg atcaagacgc
      481 agtgctgact tgatggagtc cacccagccc cgggtggtat ccataacggc tgtctgaatt
      541 gctccgggga tacggccttc cggcggggat tcaatcgtga acaacctgcg ccgtggcaga
      601 agaataccgc cggtgttgtt gaagccgtat tgttcaaggt atgccgcaga ggcggttctc
      661 agcgtggtgg attttcccga gccggtgggg ccagagaggg tgataatgcc ttcagggcgt
      721 tgcaggatac ggcgcagggt gcactcttga tccgggtgat aacccagcat actgaaggtc
      781 gggatttgct ggctgtcatc cttgatcaga cggagaacag catagaggcc tcccacgcag
      841 gggtagtggg agtagcgtgc gccaaacagg ccagccagtt tcaggtactg ttcagagaga
      901 cgtgcgtcct gtggaacccc gggatcaaat tgtgtgccct tgatgacatc cgtcatgccg
      961 gaataggtag cccccagtaa ttccagacct tcttctttcc ggaggatatc gagcacttcc
     1021 agttcgccat gaacccgggc ttccacatag gtgaagtctg tgttgtcccg ccctggcgtc
     1081 atatgaaggt cggaggcacc aagggaggca gaatggcgga tatagccgag cattttctgc
     1141 tgtatcgggt taatcgcgtt ttcatcgcct gtcgttgtgg cgtcctgtct gaggcgggct
     1201 atcacatcca gcgtggtgaa ctcaaagtct cccacacgcc cctgatagcg tgcgcg
//
LOCUS       DAZGBC010000443         1256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGBC010000443 DAZGBC010000000
VERSION     DAZGBC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.447"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1256)
                     /locus_tag="ACHK1J_005374"
     CDS             complement(<1..>1256)
                     /locus_tag="ACHK1J_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFO7914482.1"
                     /translation="RARYQGRVGDFEFTTLDVIARIREATTIAGQENGMNPVQNKMLG
                     YIRHAATLNASDLHITPGRDNTDFTYVEARVHGELEILDIIRKDEGVELLGATYSGMT
                     DVIKGTQFDPGVPQDARLAEKFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSSHIPT
                     FGMLGYHPKQEQTIRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQHGFNNTGGI
                     LLPRRRLFTIESPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGEIRDHDS
                     AITAIKAAMTGHLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLI
                     CPHCRHPWNDVASRRTEDERRLVETFCNADAVYLRNHDGCPHCWRGVTGRTVIAEVIS
                     PDARFFQLYREKGRIEAKTYWHRELGG"
BASE COUNT          267 a          339 c          352 g          298 t
ORIGIN      
        1 ccgcccagct ctctgtgcca gtaggttttg gcttcgatgc ggcctttttc ccggtacaac
       61 tgaaaaaatc tggcatcagg agagatgact tctgcaatca ctgtgcgccc tgtgacacca
      121 cgccagcagt gtgggcagcc gtcatggttt ctcagatata ccgcatcagc attacagaag
      181 gtttccacca gacgtcgttc atcctcagta cgacgggatg ccacgtcatt ccatggatga
      241 cggcagtggg gacatatcag ctgaaccaga cgctgactga gcagtccgat gaacaactgc
      301 gggtctgcaa tcatcctggc ctgaacacct tccatctcaa ggcgttcgag gatgttaatc
      361 gggtcgttcg catggatggt ggtcagcatc agatggcctg tcatggcggc ttttattgcc
      421 gtaatggcgg aatcgtgatc gcggatttca ccgttcagaa tgccgtcagg atcaagccgc
      481 agggctgatt ttatggaatc cacccagccc tgagcactgt ccatgacggc ggtctggatt
      541 gcgccgggaa tacgtccttc cggcggtgat tcaatggtga agagacgacg acggggcaaa
      601 agaataccac cggtgttgtt gaatccgtgc tgttcaagat aggccgcaga cgccgtcctc
      661 agcgtggtgg attttccgga gccggtcggc cctgacaggg tgatgatacc ttccggtcgt
      721 tgcagaatac ggcggatggt ctgctcctgc tttggatgat agcccagcat accgaaggtc
      781 ggaatatggc tgctgtcgtc cttgatcagc ctcagaacag cataaaggcc ccccacgcag
      841 ggataatggg agtaacgtgc gccaaacaac cccgcaagtt tcagaaactt ttccgccagt
      901 cgggcgtcct ggggaacacc aggatcaaat tgtgttccct tgatgacgtc agtcatcccg
      961 gagtaggtgg cccccagaag ctccactccc tcatctttcc ggataatgtc gaggatttcc
     1021 agttccccat gtacacgggc ctccacatag gtgaagtctg tgttgtcccg gcccggagtg
     1081 atatgaagat cggaggcatt aagggtggcg gcatgtcgga tatacccgag cattttattc
     1141 tgcacaggat tcatgccgtt ttcctgtccg gcgattgttg ttgcttcccg gatccgggct
     1201 atcacatcca gtgtggtgaa ttcaaagtct ccgactcgtc cctgatagcg tgcgcg
//
LOCUS       DAZGBC010000444         1254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGBC010000444 DAZGBC010000000
VERSION     DAZGBC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.448"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..372
                     /locus_tag="ACHK1J_005375"
     CDS             <1..372
                     /locus_tag="ACHK1J_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7914483.1"
                     /translation="LMLVIWSPEEIQALADGMDISLSDHEIRTVLAHLEDIPEDQRTE
                     SGISSGAAMEIIKYVSENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVT
                     RRQAVVNQARALLKNNTREND"
     gene            417..1187
                     /locus_tag="ACHK1J_005376"
     CDS             417..1187
                     /locus_tag="ACHK1J_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914484.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVQ
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVFDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
BASE COUNT          263 a          351 c          393 g          247 t
ORIGIN      
        1 ctgatgctgg ttatctggtc cccggaagaa attcaggccc tcgctgacgg aatggatatt
       61 tccctgtccg atcatgaaat caggactgtc ctggcccacc tggaggacat cccggaagac
      121 cagcggactg aatccggtat ttcttccggt gcggcgatgg agattatcaa atacgtgagc
      181 gaaaaccgcc aggtgaccgt ccctgctgaa ctgctggcgt ccctgattca gaccgctgaa
      241 caggcactgt ggaaacgtga gtgggccgcc cgtgataacg gcctcgccgt tccggaatgc
      301 gtcacccgcc gacaggcggt ggttaatcag gcccgcgccc tgctgaaaaa caacacacgc
      361 gaaaacgact gatgttatcg ccgcctgcgg gcggcgattc agggagacca tgaatcatga
      421 acgaaacgct gaatgcactg atttgtcgcc acgcccgtaa cctgctgctg gcgcagggct
      481 ggccggaaga gacggatgtt gaccagcgaa atccgaacta tccgggctgg atcagcattt
      541 acgtgcagct ggatgcgccc cggctggcga cgttactcgt taaccgccac gatggcgtgc
      601 tgccgccgca cctggcctcc gccattcaga aactgaccgg aaccggagcg gaacttgtac
      661 tgtccggcag tcagtggcag gcgctgccgg tacttccggc agacggaacg caggtgtctt
      721 tcccgtatgc cggtgaatgg ctgacggagg aagaaatcag ggcagttttt gatgcggtgc
      781 gcaatgcagt acgaagcatc tgttaccagg tggcagacga tgcgcggcgt atccgggcgg
      841 cgctgactac caccggtcag acgttgctga cccgccagac gcgccgcttt cgtctggttg
      901 tgaaggaaag cgatcacccc tgctggcttg atgaagatga cgaaaatctg cccgtggtgc
      961 tcgatgccat cctgaaccgg ggagcacgtt tttcggcggt ggaaatgtat ctggtcagcg
     1021 attgtattga gcatatcctg tccagtgggc tggcctgcga tgtgctgcgt ataccggatg
     1081 aaccgccccg ccggtggttt gaccgtggtg ttctgcggga ggtggtccgg gaagcccggg
     1141 ccgaaattcg cagcatggcg gatgccctgg caaaaatccg gaaatgatga cgctggcggg
     1201 gaaaaatctc cgccttttcc agccggtccc cggaaagagc aaaaggatgt cgcc
//
LOCUS       DAZGBC010000445         1247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGBC010000445 DAZGBC010000000
VERSION     DAZGBC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.449"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            177..638
                     /locus_tag="ACHK1J_005377"
                     /pseudo
     CDS             177..638
                     /locus_tag="ACHK1J_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            741..1124
                     /locus_tag="ACHK1J_005378"
     CDS             741..1124
                     /locus_tag="ACHK1J_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914485.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
BASE COUNT          367 a          215 c          296 g          369 t
ORIGIN      
        1 attaattcat gagaccttag taacacatgg ctatatgtgt attcagccac tgaatcttat
       61 tttactgaat agaccacaat ttaacggttt atttttggcg tgatgagtga ggcattattc
      121 atcacggaat ggaattcctg caatcacaaa aagtaatcag tgacggagag agtaaaatga
      181 agaaatatat accagtggta ttgtttgttt tttcatatca ggttttttct gctgatattc
      241 atggacgggt cgtgcgtgtt ctggatggcg ataccattga cgtcatgtta tctcagcatc
      301 cggtgcgtgt ccgtcttgtt aatattgatg cacctgaaaa gaaacaggac tatggacggt
      361 ggactgaaaa aataatgtaa tctctggttg cgggaaaaac ggttactgtc acatattttc
      421 agcgagatcg ttatggtcgt attctcgggc aggtttatgc accagacgga atgaatgtta
      481 atcagtttat ggtccgtgca ggcgctgcat gggtttatga gcagtacaac accgatcctg
      541 ttcttcccgt cctgcaggaa gaagcacgac agcagaaacg tggtctgtgg gcagacagcg
      601 cgcctgttcc gccctgggta tggcgctcac ggaaataatg atatttctgt ggctattgag
      661 ttctcattga aatgcgatat ttaaaagtat gaagcatatt acattattaa cttatttctc
      721 tgttaatgaa aggtgaacaa atgaactatc aggcagtatc agaattaatc acatcgtcta
      781 atcataatgt tcttattgtg ggggactcag cttctgaagt tgatagcttt ttaaataaac
      841 tgaatattac tgattataag tattatgatt tcagccagat ttacagttgt tctgacagaa
      901 cattaaatga ttatgcggtt atttttatca gggatgcact gaacgccagt gagcatatta
      961 ttatttttaa ttgtacagga tggcctgatt tgaataatga atctgccgtc atgcagtttg
     1021 ccagagtggc acgaaaatca ggaaaacaac tgatagtcgc agtaagagaa caggatatga
     1081 aaaaaatgga agcagagttt ggaaggatca taaaaatcca ctgacgcctt cgcgttgctc
     1141 agttgtccaa ccccggggac gggaaaaagc aagttttccc cgcccccggc gtttctgtag
     1201 tgaaaagcgg gaaatggagg gaatggtcag taacagggaa tgtggta
//
LOCUS       DAZGBC010000446         1246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGBC010000446 DAZGBC010000000
VERSION     DAZGBC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.450"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..313
                     /locus_tag="ACHK1J_005379"
     CDS             <1..313
                     /locus_tag="ACHK1J_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO7914486.1"
                     /translation="KPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIY
                     ATHRRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQFSSEDKFIEGEDTEIENKDN
                     N"
     gene            complement(384..677)
                     /locus_tag="ACHK1J_005380"
     CDS             complement(384..677)
                     /locus_tag="ACHK1J_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO7914487.1"
                     /translation="MSLVGYNSKIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLLISQDRLTH"
     gene            complement(679..1179)
                     /locus_tag="ACHK1J_005381"
     CDS             complement(679..1179)
                     /locus_tag="ACHK1J_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7914488.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRKLKPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
BASE COUNT          410 a          204 c          223 g          409 t
ORIGIN      
        1 taagccacat ctttcaccta ttccattgat ttctgtaaga gaaactaatg aggtagcctt
       61 agccgtaagg aaatatgcaa aggaaatcgg aataccaatt atcacagata aaaaattagc
      121 acgaaaaatt tatgctaccc atcgtcgcta cgattatgtt agcttcgaaa atatagatga
      181 aatattacgt cttctgctgt ggcttgaaga tgtggagaat gccggacaac ctgttccaga
      241 tgaacagttc tcttcagaag ataaattcat tgagggtgaa gacacagaaa tcgagaataa
      301 agataataat tgaataaatt aatttctatc gtagtcagat atttaaaaat aatctacacg
      361 caaaaagcgt gtagattcat tatttaatga gttaaacgat cttgcgaaat gagaagcaat
      421 tgatacatac tcatctcttt ataagctgga actgtagtaa cctgtgcaaa ttcgcgatat
      481 agcttttgct ccagttctgc tgaagatata tattccgggt gttcgctaaa gaactgtctt
      541 gcatctacta tcgcttggga ttcgccactt cttttatttt gattctcctt taatgaattt
      601 tctctggtgc atgcagagag caacgatatc agcgcgatgg ttattatgat tttactatta
      661 tatcctacta atgacatatc aactttctct tacgcaagat tggagtttaa aataatgttt
      721 atcccattca cagagtgcta taaacaactc aacagtacct tttatgttaa gtttatttag
      781 ggctcttatt ttgtgatatg atactgtttt ataatcaatt cctttaataa tggctatttg
      841 ttttaacgag taagaaacac ataaccgcag gatatctatc tcatcatggg taagtggttc
      901 ttgtgtggaa tacacatttt gtgtatgaag attcgaaatc aggaaatgcg ttaaatcaga
      961 taacgtaata ttcttattga tcctgatgaa attgttgctt aaaaggatgt ttacaggttc
     1021 tgtatttggt ttgagctttc gatgttgatg ctcatcaata acaacgaacg aatgcccaag
     1081 atcaaccaac gcaactaaat cgctgtaata ttctttttgg gatgctaata atgcattagt
     1141 gcctacagtg aagtagacat cactggaaaa gacttgcata tgccaacctg tactctaaaa
     1201 tcatttaggg taaatattac gtgtaaaagt taaaataaga gtaatt
//
LOCUS       DAZGBC010000447         1238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGBC010000447 DAZGBC010000000
VERSION     DAZGBC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.451"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            165..689
                     /gene="lpfA1"
                     /locus_tag="ACHK1J_005382"
     CDS             165..689
                     /gene="lpfA1"
                     /locus_tag="ACHK1J_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO7914489.1"
                     /translation="MKKVVFALTALALTSGSAFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADQSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            754..>1238
                     /locus_tag="ACHK1J_005383"
     CDS             754..>1238
                     /locus_tag="ACHK1J_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7914490.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSATRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFRLDPGKNNILRIVNTTPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLQGDVKTAPNELRFTRSGNQI
                     RV"
BASE COUNT          330 a          262 c          289 g          357 t
ORIGIN      
        1 acttagtgat ttaaatcaaa attcacactt tcttaattta ttgatatttt atcctcttaa
       61 ttttcttttc cttgtttatt tgtataatcc cgccttcaaa gttgatgcaa tgttaaagca
      121 tcactatttg ctaattaatg tgatttaata aaatggagtt tttcatgaaa aaggtcgttt
      181 ttgccttaac tgcactggcg ttaacctccg gctctgcatt tgcagcagaa tctggtgacg
      241 gcacggttaa atttacaggc gagatcgtgg attcaccttg cgtactgtcc gttgactctc
      301 agaaccagga agttgtgttg ggtcaggtac aaaaatctgt atttgctgcg gttggtgaca
      361 aatccccggc aaaacctttc gaaatcaaac tggaagattg cgataccacc acgatgaaaa
      421 aagccaatgt gagctttagc ggtgttggtg atgcagatca atctgatctg atttctgtta
      481 gtactgaagc cggtgcggcg aaaggtgttg gtatcggtat ttatgataac agcaacaccc
      541 tagtcgctct taacggtggt aaagcaagtg ttgatctgag taaaggccag acggttctct
      601 actttaccgc caactacgtt tcaactctgg caacggtaac gaccggttac ggcaatgcgc
      661 aggtcgattt caacctgtct tacgagtaat cgtctgtttt acccatggcg gcgtaattgc
      721 cgccattagt cgtcaaaatt catggatcga atgatgaaaa gtaaatttgt tgcgttggct
      781 ctgtctctgt tcctctccca gtcagttctg gcggggggcg ttgggcttag tgctacccgt
      841 gtgatttatg atggcagtaa gaaagaagcc tcgctgaccg tacagaataa aagtaagacg
      901 gaagagtttc tgatccagtc ctgggtggat gatgccgcgg gcagcaaaaa aacaccgttt
      961 ataattaccc cgccgttgtt caggctcgat cctggaaaaa acaatatatt gcgtattgta
     1021 aatactaccc ctggcctgcc gcaggatcgc gaatcggtct actgggttaa tgtgaaagcc
     1081 attccgtcta aaagtgacga cagcgaaaac aaaaatgtat tacagattgc tgttcgcacc
     1141 cgcatcaaac tgttttatcg cccggcaggg ttacagggtg atgtaaaaac cgcgccaaac
     1201 gaactgcgat ttacgcgtag cggtaatcag atccgtgt
//
LOCUS       DAZGBC010000448         1238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGBC010000448 DAZGBC010000000
VERSION     DAZGBC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.452"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(50..283)
                     /locus_tag="ACHK1J_005384"
     CDS             complement(50..283)
                     /locus_tag="ACHK1J_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914491.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(379..1002)
                     /locus_tag="ACHK1J_005385"
     CDS             complement(379..1002)
                     /locus_tag="ACHK1J_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7914492.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYWYHLSHRIRRMVGSENTSSPYQQIAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            complement(1091..1238)
                     /locus_tag="ACHK1J_005386"
                     /pseudo
     CDS             complement(1091..1238)
                     /locus_tag="ACHK1J_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247776.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          303 a          349 c          277 g          309 t
ORIGIN      
        1 gaaccccctg ccggcaatca cactgcctga tggcagcata atccacttat taattctcat
       61 ccccggagac aagcccagaa aaccactcgg gatgttcact gtatgccgtg ttcagcgcat
      121 cccaggaaac gttgatataa aagctgtcct gcaatttgcg ggtaacaatt tctgccaccc
      181 ggttacggtt atccgcctgt ccggcataac ctctctctgc cagccactca ttcagttcat
      241 gttccggaga cagattgata atatcgtaat cggaccttgc cattcccgtt tccttagcac
      301 atgttttcag gcacggggat aaagtataaa gaatgtcgta ctgctgctgc cagagcaata
      361 ttccccctga tggtcgtatc agagatggta tgccggtatt ttgcgggttg ttcccgtcag
      421 gttatcgctc acttccacgg acgttgtcac caccggcatt ccggcctttc tcagcctcaa
      481 aacatcagct gcaatacgct gactaccaga ccagaacagg ccgtccagtg cagtcaccag
      541 taaccccgcc tccagcgcat acttcagccg ttcacggggc gctttcactt cccgggcaat
      601 ctgctggtat ggcgatgatg tattttcact cccaaccatc cggcgaatac gatgagacag
      661 atgataccag tatgtatccg gcacaccgga aaggctggcc ttcaggctgt acacgcagcc
      721 aaagcgttta tcgttgaaca ccacattttt ctggctgata tcccattctt cacgcagcgc
      781 ggcaatcagt tgtggtgtac gggtaagcaa caagcgaaaa ggcagttcaa aactggtgac
      841 gtaatccaca ttcagcaggg caatgcgcag tcgttcttct ggtccggctt ctgtctgccg
      901 gcactcctcc aggacatcct gccactgcag gcgaagacgg gaagactcat tcagttctgt
      961 aaagcagtat ttatccgcca gataatcaat tcgtgtatgc atactgaaga gtattccgta
     1021 taaagattca gctggcaaaa ctttatcagt ctgtaaaaac taacggaaga gtcgatattt
     1081 ctcccgtcca tcaccggatg attgttgcaa taccccgtgg catcagagac tgaacagccg
     1141 tttttaacgc aacgtattgc tctgtcagcg ccagcgctta tcaccggcca ggtctgttgc
     1201 agtaaatccg ccacttgcga acatgcttca tcaactgt
//
LOCUS       DAZGBC010000449         1230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGBC010000449 DAZGBC010000000
VERSION     DAZGBC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.453"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..226
                     /locus_tag="ACHK1J_005387"
     CDS             <1..226
                     /locus_tag="ACHK1J_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7914493.1"
                     /translation="TNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYVM
                     KIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            257..796
                     /locus_tag="ACHK1J_005388"
     CDS             257..796
                     /locus_tag="ACHK1J_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO7914494.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            793..>1230
                     /locus_tag="ACHK1J_005389"
     CDS             793..>1230
                     /locus_tag="ACHK1J_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7914495.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNIGINNVISEWS"
BASE COUNT          335 a          285 c          326 g          284 t
ORIGIN      
        1 gacaaatcaa accattcaac tcgcaatcag tattacaggt agtcaaaaac gactggcaga
       61 tctatgcggt gtagcccagc ccactgtttg gcgttggcta cacggtggcg gaattgatgc
      121 ccgttatgta atgaaaattg tctcagccac tggtggaaag attaaaccag cagatattcg
      181 tcccgacctc gcaccattgt ttaacgcgag taattctgcc gcctaatctg cggcgttaac
      241 tgataaggca atgactatgc aaccacttac ataccaacag actagcggat ttagcccgac
      301 tgcggtgata aatcgttctc aaacaaaaca agctccaggc cacgaaaaaa tccgtgatgc
      361 cgtccgcgcc tggtcggctg cagataatca ggatgttgtt gccgcactca ttgtgaatga
      421 gtatcgggag cagggcggcg gcaccatcga tttccctgat gatgtcagcc gtgcacgcca
      481 gaagctgttc cgcttcctcg ataacaaatt cgattctgaa aaataccgaa ataacgtgcg
      541 tgaactgacc ccggcaattc tggcggtact accgctggaa tatcgcggcc acctggttga
      601 gcaggatagc ttcatggctc ggctggctga aatggaaaag gaactcagtg aggcaaaaca
      661 ggctgtcatt ctcaacgcac cacgccacca gaaactgaag gagatgagtg aaggcattgt
      721 gtcgatgttt cgtgtggacc cggatctggc tggtccactg atggcgatgg tcaccaccat
      781 gctgggggca atatgacagg ttcggaaatg gcgaaagccg gtctgcgcga acagaaccga
      841 ctttcaggtg caaatcgtaa cacactcatt gcgggaggaa ttatggcaaa cactgctgag
      901 atattcaatt ttccagtgcc ggatgtggca caaaaggagc ggcgcgtggc agatctcgat
      961 gatggttata cgcgcattgc aaatgagttg ctggaagctg tgatgctggc cggattaaca
     1021 cagcaccagc ttctggtctt tctggctgtc atgcgcaaaa catatggctt taataaaaaa
     1081 ctggattggg ttagcaacga gcaactttcc gaattaaccg ggatattgcc gcacaagtgt
     1141 tctgctgcaa aaagtgttct ggtaaagcgt gggattttga ttcagagcgg gcggaatata
     1201 ggcattaata atgtgatcag tgaatggtca
//
LOCUS       DAZGBC010000450         1227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGBC010000450 DAZGBC010000000
VERSION     DAZGBC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.454"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            440..>1227
                     /gene="iolR"
                     /locus_tag="ACHK1J_005390"
     CDS             440..>1227
                     /gene="iolR"
                     /locus_tag="ACHK1J_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFO7914496.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASM"
BASE COUNT          339 a          259 c          277 g          352 t
ORIGIN      
        1 ataagcccac acacatgtaa cgtactgcag atactcgctt ggcagagttg ttacttcttg
       61 ggaggcagta ccagccatca gatattcccg ttccatgcca aaacctgagt tacccggata
      121 ccattacatt gcctgtattt cattcatctg ccacacacgt gtcagctctt tgtgacctga
      181 cgcaatcatc tgactcagca gcgattcacg gtactggtct ttttggtgac cgttctgaca
      241 gagcaataca gtgggacaaa cgaccatcgt catcactgga gaggcgtgca tggaaactcg
      301 tcaacaactc aagttcgccg gaacggaacg ttaacaatag aactttactg tataaatttg
      361 aatactgcta aatgtatgaa tggttcattc atgttaatat tgtgatttgc aaaaatttca
      421 ttttattaga gtaaaataca tgtctaaaca ccaaacacag ctttccttac tgcaggacga
      481 catccgcagt cgctatgaca gtctcagcaa acgtcttaaa caagttgctc agtatattct
      541 ggataacagt aatagcgttg cttttgatac ggttgcttct attgcgcagc acgcggaggt
      601 acctccttcg acattaattc gttttgccaa tgcgtttggt tttagcggat tcaatgaaat
      661 gaaacaagta tttcgccagc atttgatgga ggaaaccgtt agctatacag aacgggctcg
      721 tctgttcaga caaaaaaaca tcaatgatgg ggaacccaca ccggaaaaac cgggcgaaat
      781 tctgaagcta ttttccatgg taaatgcaca ggtactacaa caattaccga tgcagattaa
      841 aaatgagcaa ctggatgctg cagtaagttt attgtcaaaa gccgataata tttatgtgat
      901 tggattacgt cgttcattta gcgtggcctg ttatttaacc tatgcattgc gccatcttga
      961 gaggcgtgca tttttaattg atggtattgg aggcatgttt tctgagcaac tgagtatgat
     1021 cagctctaac gatgtagtca tcgccatcag ttattcgcca tacgctcagg aggttgttga
     1081 actggttgag ttgggggcaa aacgtgatgc acatcatatc gtcattaccg acagtcaggt
     1141 cagtccgttg gctgcgttca gtgaggtctg ttttgtaata cgtgaagcgc aggtggatgg
     1201 cttccgttcg caggtagcgt cgatgtg
//
LOCUS       DAZGBC010000451         1226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGBC010000451 DAZGBC010000000
VERSION     DAZGBC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.455"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..780
                     /gene="yeeO"
                     /locus_tag="ACHK1J_005391"
     CDS             <1..780
                     /gene="yeeO"
                     /locus_tag="ACHK1J_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO7914497.1"
                     /translation="GAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPAS
                     VESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLG
                     VGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNA
                     LFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMG
                     MFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            881..956
                     /locus_tag="ACHK1J_005392"
     tRNA            881..956
                     /locus_tag="ACHK1J_005392"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:914..916,aa:Asn,seq:gtt)
BASE COUNT          281 a          248 c          327 g          370 t
ORIGIN      
        1 ggcgcagttg caattttgtg ggtgctggcg attggtttta atcctgcgct aaggatttcg
       61 ttaaagagct attttaaacc gctgaatttt agcattatct gggaagtcat ggggattggt
      121 attccagcga gtgtcgaatc agtgttattt accagtggtc ggttattaac ccaaatgttc
      181 gttgccggga tggggaccag tgttattgcc ggaaatttta tcgcgttttc aattgcggct
      241 cttatcaact tacccggaag tgcgctcggc tctgcttcta cgatcattac aggccgaagg
      301 ttgggggtag ggcagatagc gcaagcagag attcagttgc ggcatgtgtt ctggctgtcc
      361 actcttggat taacggccat cgcctggcta acggctccct ttgccggggt tatggcatcg
      421 ttttacaccc aggatccaca ggttaaacat gtcgttgtga ttctgatttg gctaaatgct
      481 ttatttatgc ctatttggtc cgcctcatgg gtgctacccg ctggatttaa aggtgctcgt
      541 gatgcccgtt acgccatgtg ggtttcgatg ttgagcatgt ggggttgtcg ggttgtcgtc
      601 ggttatgtgc tgggaatcat gcttggctgg ggtgtggttg gtgtctggat gggaatgttt
      661 gccgactggg ctgtgcgggc cgtgctgttt tactggcgaa tggttactgg acgttggcta
      721 tggaaatacc ctcgatccga gccgcaaaag tgtgaaaaaa agccagctgt gtcggaataa
      781 acgacaaaat gcagattatt tcagcaaacg atttcaaatt taaaaaacag gctttgacat
      841 tgtgggtggg catcgctaat attcgcctcg ttctcacgat tcctctgtag ttcagtcggt
      901 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
      961 ttagctttga gacatctcta tgagtctcaa aacagttaaa aaataaaaat tatttttacc
     1021 cggtcacttt gtaaaaaaaa gtgaccgggt tttttgattt ctgtgtttcg tgcgcttaat
     1081 cgagtctcac gcttcagtga gcaaattgcc cctccccaac agacatcacg attcacaagg
     1141 tcaaaaattc tgttccatga agtaccgtac aaagataaag agaaaaatgc cttatgcggg
     1201 atgtcgctgt cgcgtcttta tcggga
//
LOCUS       DAZGBC010000452         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGBC010000452 DAZGBC010000000
VERSION     DAZGBC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.456"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..1200
                     /locus_tag="ACHK1J_005393"
     CDS             join(44..293,293..1200)
                     /locus_tag="ACHK1J_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO7914498.1"
                     /translation="MSRKIQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDIFKKSNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          358 a          287 c          311 g          266 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaatcca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac tggaatctga
      241 aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt gagcgagata tattaaaaaa
      301 agcaacagcg tattttgcac aggagtcgct gaaaaatacg cgttaatcga acaatggcga
      361 caacaatttc ccattgaagc gatgtgtcag gtatttggtg tatccaggag cggttattac
      421 aactgggtac agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag
      481 attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag
      541 ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta
      601 aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaactcgaa ccacaatctg
      661 ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg
      721 gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa
      781 gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg
      841 acaggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg gctaatccac
      901 cactccgatc gtggttcaca gtactgcgca tacgattacc gggtcataca ggagcagttt
      961 ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc
     1021 ttctggggaa cgctgaaaaa tgagagcctg agccactatc gttttaataa ccgggatgaa
     1081 gccatctcag taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct
     1141 cgtctgggga atatctcccc ggcagccttc agggaaaaat atcatcagat ggctgcttaa
     1201 aaaaagaaca aatggtagtg tc
//
LOCUS       DAZGBC010000453         1215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGBC010000453 DAZGBC010000000
VERSION     DAZGBC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.457"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1215
                     /locus_tag="ACHK1J_005394"
     CDS             <1..>1215
                     /locus_tag="ACHK1J_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HFO7914499.1"
                     /translation="PLKEASSQSEEPHQLVNSKSEGNLHNSASKDNNIGAMLKKDVEK
                     LSTLVSERSPRNEEWKCQHDIQLNFVKNMLRNFPTENLSEKGSALLHESYLLIKNVLQ
                     LTENVNTIDGKLKDPAKILTLHNAVRKTLANVAHNTTGPGAMATTKGTLTHVIASLDS
                     FAPEMRSSATSSTLTASSSSEFKSDKYICSDIADLMSVLGSDYLEIYANSVEIMSAYW
                     QDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHFYYIDKYPNGDFHYHYNPDYV
                     LYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTPSPIVEVEPQGSGLYVIIPNP
                     QIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAEYQAWLAGYNGQAENMKTDVQ
                     VITTKYSTANST"
BASE COUNT          410 a          254 c          253 g          298 t
ORIGIN      
        1 tcctctcaaa gaggcatcct cgcaaagtga ggagcctcat caactggtaa acagcaaaag
       61 cgagggaaat ttgcataaca gcgccagtaa ggataataat attggcgcta tgctcaagaa
      121 agatgttgag aaactatcaa ctttggtcag tgaacgtagc ccgcgaaatg aagaatggaa
      181 atgccagcac gacatacaac ttaacttcgt taaaaacatg ctcagaaatt tcccaactga
      241 gaacctgtca gagaaaggat ctgcgctctt acacgaaagt tatttgctga taaaaaacgt
      301 tctgcagctt acagaaaatg ttaatactat tgacggaaaa ttaaaggatc ccgctaaaat
      361 attgaccctg cataacgctg tgagaaaaac cctggctaac gttgctcaca acacaaccgg
      421 gccaggtgca atggcaacga ccaaaggaac cttaactcac gttatcgctt ctttagatag
      481 ttttgcgcct gaaatgagaa gttcagcaac cagcagtact ttaacggcca gtagttcaag
      541 cgaatttaaa agtgataaat atatatgtag tgatattgct gatttgatga gtgtgctcgg
      601 tagtgactac ctcgaaatct atgcaaatag cgttgagata atgagcgcat actggcagga
      661 cttcagtgaa catatccaaa gcaatatggg aaaatggact cactctaata aaaaaggcga
      721 tgcgatagtt tttgatgtca atgcattcca aaaagccttg atgcattttt attatatcga
      781 taaatatcct aatggtgatt ttcattatca ttacaatcca gactatgttt tataccctcc
      841 ggcacctgct gacaaaattg gtgttccact ggaagaagca gaaaaatggt gcgccgcttt
      901 aggacttccg gttattccac ccgacccaaa acaccgcaca ccttctccta tagtcgaagt
      961 tgaaccgcaa ggtagcggtc tgtatgttat tattcccaac cctcagatta ttgacagtat
     1021 gagccagtct tcagactcaa tggttcacag ggatgataaa gggaaggaaa aaaatatatc
     1081 taaagaattc accggctacg aaataagcac ggctgaatac caggcatggt tggcgggtta
     1141 taatggccag gcagaaaaca tgaaaaccga cgtgcaggtt ataacgacta aatacagtac
     1201 agccaactcc accta
//
LOCUS       DAZGBC010000454         1213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGBC010000454 DAZGBC010000000
VERSION     DAZGBC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.458"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1213
                     /gene="espI"
                     /locus_tag="ACHK1J_005395"
     CDS             <1..>1213
                     /gene="espI"
                     /locus_tag="ACHK1J_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFO7914500.1"
                     /translation="AVGLLSGFNTAYRGAIHAPSASATVNNTWWQLTGDSSLRSLKNT
                     GSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLVVNKKLSGKDNILLVD
                     FLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITAIDAGDKTTWNLTGY
                     RMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAG
                     GGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADAFKGKTKSVGAGLYASA
                     MFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRCHVTEDTWIEP
                     QAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPLIGRTGVDVGKSFSGKDWKVTARAGL
                     GYQFDLLANGET"
BASE COUNT          326 a          272 c          331 g          284 t
ORIGIN      
        1 ttgcggtcgg attactgagt ggttttaata cggcataccg cggtgcaatt catgccccgt
       61 ctgcatctgc cactgtgaac aatacctggt ggcaactgac aggagactcc tcacttcgct
      121 cgttaaaaaa taccggaagc atgacatatt ttacaggaag tgcagcgaat aaagcattcc
      181 atacactgac ggttgatgag ctgacgacga atggcactgc gtatgccatg cgtacggacc
      241 tgaaaaatgc ggataagctg gtagtaaata aaaagctgtc aggtaaggac aatattctgc
      301 tggttgattt tctgaacaaa cccaccggag aaaaactgga tattgaactg gtgagtgcac
      361 cggggaacag cagtaaggat gtttttaaag gaagtgaaca ggcaataggt ttcagcaatg
      421 tcacacctgt tattacagct atagacgccg gagataaaac aacatggaac ctgaccgggt
      481 acaggatggc agaaaatcct gccgcaaccc aaagtgcctc aggccttgca tctgtggggt
      541 acaaatcatt tttgagtgag gtcaacaacc tgaataaacg tatgggtgac ctgcgtgaca
      601 tcaatggtga agctggcgca tgggcacgta tcatgagcgg aaccggctct gccggtggtg
      661 gtttcagtga caaccacaca catgttcagg tcggtgtcga caaaaaacat gagctggacg
      721 gactggattt gtttaccggc ttcactgtca cacacactga cagcagtgcc tccgctgatg
      781 ctttcaaagg taaaacgaag tctgtggggg ccggactcta tgcttctgcc atgtttgatt
      841 ccggtgccta tatcgacctg attggtaagt atgttcatca tgataatgag tacacggcaa
      901 cctttgccgg actcggaacc cgtgattaca gtacgcattc atggtatgcc ggtgctgaag
      961 caggctaccg ctgtcatgtc actgaggata cctggattga gccacaggca gaactggttt
     1021 acggtgctgt atccggtaaa cagtttgcat ggaaggacca ggggatgcat ctgtccatga
     1081 aggacaggga ctacaatccg ctgattggtc gtaccggtgt ggatgtgggt aaatccttct
     1141 caggtaagga ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg
     1201 ctaacggcga aac
//
LOCUS       DAZGBC010000455         1190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGBC010000455 DAZGBC010000000
VERSION     DAZGBC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.459"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..520
                     /gene="tssB"
                     /locus_tag="ACHK1J_005396"
     CDS             23..520
                     /gene="tssB"
                     /locus_tag="ACHK1J_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7914501.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSYG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            541..>1190
                     /locus_tag="ACHK1J_005397"
     CDS             541..>1190
                     /locus_tag="ACHK1J_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7914502.1"
                     /translation="MLMSVQKENNVAGNVVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVG"
BASE COUNT          303 a          251 c          347 g          289 t
ORIGIN      
        1 gcaggtttca caggagaacg tcatgagcag ttcgtttcag aatgagatcc cgaaggcaag
       61 ggtgaatctg aaattagacc tacatacagg tggtgggcag aagaaagtgg agcttccgct
      121 caaactgctg gttgccggtg atttcagtta cggacaggaa gagcgcccgc tgtcagagcg
      181 cataaaaatg gatgttaata aaaataactt taatgctgtt ctggctgata tctcgccgtc
      241 agtacgcctg aatgtggaga acacacttgc cggtgatggt actgaagaga gcatcagcct
      301 gactttccgt aatatgaacg atttcactcc ggagcaggtt gcccgacaaa ttccccgtct
      361 taaagccatg ctggccatgc gaagtttgct gcgggatctg aaggcgaatc tgctggataa
      421 cgccactttc cgtaaagaac tggaaaaaat tctgcgggat cccgaactga gtcaggcctt
      481 acgtgacgaa ctgagtgcac tggtgccgga acaggtgtga ttaacggatt aagagagagc
      541 atgctgatgt ctgtacagaa agaaaataat gtggcgggaa atgtggtttc tgaaacgcat
      601 tccggcgaca gcgtatatgc ttccctgttt gaaaaaatta atctgaatcc ggtatcagcc
      661 ctgagtgcgc tggatatctg gcaggatccg caggccatgt cagaggcatc agcagatgaa
      721 cgtctgaccg cagggatgca ggtgtttatg gaatgcctgg cgaaagcggg agcaccggtg
      781 gagaaactgg ataaggcgct gattgaccat catattgcag agcttgatta tcagatcagc
      841 cgtcagctgg atgccgtgct tcatcatccg gaatttcaga aagtggagtc cctgtggcgg
      901 ggcgtgaagt ctctggtgga taaaacggac ttccgccgta atgtgaaaat tgaactgctg
      961 gatctgtcca aagacgatct gcgtcaggat tttgaggatg cgccggaaat tattcagagt
     1021 ggtctttatc tgcagactta tgtggcggag tacgatacgc cgggtggtga acccattgca
     1081 gccctgattt ctgcctggga gtttgatgcg tctgcgcagg atgtggcgct gctgaaaaat
     1141 atttcccggg tggctgcgtc cgcgcatatg ccatttattg gttcagttgg
//
LOCUS       DAZGBC010000456         1189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGBC010000456 DAZGBC010000000
VERSION     DAZGBC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.460"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1189
                     /gene="rhsD"
                     /locus_tag="ACHK1J_005398"
     CDS             <1..>1189
                     /gene="rhsD"
                     /locus_tag="ACHK1J_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO7914503.1"
                     /translation="EPAEQWQYDGHGWLTDISHLSEGHQVAVHYGYDDKGRLAGERQT
                     VHNPETGELLWQHETEHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVE
                     YTRDRLHRETVRSFGSMAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWSDNGDL
                     VRISAPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTA
                     WPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRI
                     QHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYDWDGDRLTTVQT
                     DTTRIQTVYEPGSFTPLIRVETENGEREKVQRRSLAEKLQQEGSEDGHGVVFPAELVR
                     LLD"
BASE COUNT          265 a          347 c          423 g          154 t
ORIGIN      
        1 gtgaaccggc agagcagtgg cagtatgacg gccacggctg gctgacagac atcagccacc
       61 tgagcgaagg ccatcaggtg gcggtgcatt acggttatga tgataagggc cgcctggccg
      121 gggagcgcca gacggtgcat aacccggaga cgggggaact gctgtggcag catgagacag
      181 agcacgcata caacgaacag gggctggcaa accgcgtcac gccggacagc ctgccgccgg
      241 tggagtggct gacgtatggt agcggttacc tggcgggcat gaagctgggc gggacgccgc
      301 tggtcgagta tacgcgggac aggctgcacc gtgagacggt gcgcagcttc ggcagcatgg
      361 caggcagtaa tgccgcatac gaactgacca gcacatacac ccccgcaggc cagttacaga
      421 gccagcacct gaacagcctg gtatatgacc gtgactacgg gtggagtgac aacggcgacc
      481 tggtgcgcat cagcgccccc cggcagacgc gggaatacgg ctacagcgcc acgggcaggc
      541 tggagagcgt gcgcaccctc gcaccggacc tggacatccg catcccgtat gcgaccgacc
      601 cggcgggaaa ccggctgccg gacccggagc tacacccgga cagcacgctc acggcgtggc
      661 cggataaccg catcgcggag gatgcgcact atgtctaccg acacgatgaa tacggcaggc
      721 tgacggagaa gacggaccgc atcccggcgg gtgtgatacg gacggacgac gagcggaccc
      781 accactacca ctacgacagc cagcaccgcc tggtgttcta cacgcggata cagcatggcg
      841 agccactggt cgagagccgc tacctctacg acccgctggg acggcgaatg gcaaaacggg
      901 tctggcggcg ggagcgtgac ctgacggggt ggatgtcgct gtcgcgtaaa ccggaggtga
      961 cgtggtatga ctgggacgga gacaggctga cgacggtgca gactgacacc acacgtatcc
     1021 agacggtata cgagccggga agcttcacgc cgctcatccg ggtcgagaca gagaacggtg
     1081 agcgggagaa agtgcagcgc cgcagcctgg cggagaagct gcagcaggaa gggagcgagg
     1141 acgggcacgg cgtggtattt ccggctgaac tggtgcggct gctggacag
//
LOCUS       DAZGBC010000457         1183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGBC010000457 DAZGBC010000000
VERSION     DAZGBC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.461"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..909
                     /locus_tag="ACHK1J_005399"
     CDS             <1..909
                     /locus_tag="ACHK1J_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO7914504.1"
                     /translation="KGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAALIRNGEE
                     RGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIPIVSAFA
                     PEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLAIIIRFSLSRYTGPGRRLLDRLPPW
                     SLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKNVLSGQN
                     LATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKSGLIITG
                     ISGGFVFFFILNMISAIYSISDMLKQ"
     gene            983..>1183
                     /locus_tag="ACHK1J_005400"
     CDS             983..>1183
                     /locus_tag="ACHK1J_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO7914505.1"
                     /translation="MNFRARLSSYFSLKKDKEEDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIAT"
BASE COUNT          345 a          280 c          258 g          300 t
ORIGIN      
        1 aaaggaacat ccggcatgta ctttctggcc agagaatgta ttgcctccct gaaagccagc
       61 ggtatgctgt cgggggccct gcccacatgg gttcccccgg aagaagcggc actgatcagg
      121 aacggtgaag aacgcggaaa catcgccgaa gccctgttcc aggtcgcaaa aaccgtgaaa
      181 gcacggcggg aaatggtgag cagtctcatc acactctgtc tttatcccct tctgctgctt
      241 tcactctgtg tggtgaatat gtacaacgca catacccgca tcattcccat tgtcagcgct
      301 tttgcaccgg aggagaaatg gtcattccag atgaaagtcc ttgcagccat gtcagagctg
      361 gtcatcggct acggtctgtg gctcagtggt gccatcatca cgcttgccat catcatccgc
      421 ttctcactca gtcgctatac cggcccggga cgcagacttc ttgacagact gccaccatgg
      481 tcgctctatc agacttttca tggtgtgaat tatctgttca gcatatcgtc aatgctgcag
      541 ataaatattc cgctttcaca ggcgctgctc agaattgaaa aaagtgcgga gcataataaa
      601 tggttaaagg agagaattca ggcaataaga aaaaatgtat tatccggtca gaatctggcc
      661 accgccatgc gcaattctga ctatgatttt ccttcaaaaa tatgcatcaa taaacttctc
      721 ttaaattctg aaagggaaga cagcgtatct gcaatgaata cctatgccgg acagtggctg
      781 gaagatgcaa aaaagagcgt cagaaaaagt ggattaatta tcaccggtat ttcaggaggt
      841 tttgtattct tttttattct taacatgatt agcgcgatat acagtatcag cgatatgctc
      901 aagcaataat acctgccgta acactaaaac gcccttcaat atacttccat taacgtaaca
      961 accattaata caggagtcat atatgaactt cagagcacgt ctgtcttctt atttttcttt
     1021 aaaaaaagat aaagaagaag ataaaggttt aacattatta gaaattgccg gcgcaatgat
     1081 tatcatcgca atagttgtcg cgctcgcgat gagccagatg tccagcacaa tggcctccag
     1141 tgataaccag cgcgaactga aaaacctgca gacaattgcg acc
//
LOCUS       DAZGBC010000458         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGBC010000458 DAZGBC010000000
VERSION     DAZGBC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.462"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            201..383
                     /locus_tag="ACHK1J_005401"
     CDS             201..383
                     /locus_tag="ACHK1J_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7914506.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            409..681
                     /locus_tag="ACHK1J_005402"
     CDS             409..681
                     /locus_tag="ACHK1J_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7914507.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEACLDAEVDA
                     ILDELLGVPAEPPTEAGDTTAESGELQPESPVSDATEPQPESVMML"
     gene            757..972
                     /locus_tag="ACHK1J_005403"
     CDS             757..972
                     /locus_tag="ACHK1J_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7914508.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            976..>1180
                     /locus_tag="ACHK1J_005404"
     CDS             976..>1180
                     /locus_tag="ACHK1J_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914509.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSTLMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNFS"
BASE COUNT          320 a          257 c          335 g          268 t
ORIGIN      
        1 catatacgct gctgattgac agtgtgaagg tggaagtgaa caacgcggac gtcgcggcat
       61 gataaaaaaa gccgccagcg gcaggaatgg aagctggcgg aggtaatccc aatggagaat
      121 gtaaagaaaa gatgctttcg tacatcggtt gtttttttaa tgaaaacagt tctcattgtc
      181 aaccataacg gtaagaaatt atgacattta ttcatcaggt gatgttgtac ttctgtacgg
      241 cggtctgtgt gctgtatctc ctttcgggtg ggtacagggc agtgcgcgat ttctggcgca
      301 ggcagattga taaaagggcc gcagagaaaa tcagcgccag tcagtcagcc ggaagcaaac
      361 ccgaagagcc cgttactcct taacaacccc tttcagcgag aaaatcccat gtcagaaatc
      421 acatccctgg tcactgcaga ggcagtgaag gaagtcctgc gctctgaaga agtcctgagc
      481 gcactgaaac agaaactccg ccagaacctt gaggcgtgtc ttgatgcaga agttgatgcc
      541 attctggatg agctgcttgg cgtaccggca gagccaccga ctgaagcggg agataccacc
      601 gcagagagcg gtgaacttca gcctgaatca ccggtctccg atgcgactga accgcaaccc
      661 gaatcggtca tgatgctgta acggggtgtc ggggccatca gtaaactgct gctggccttt
      721 ttcatgttgt gagcttccgt gtgcgggaga cggggtatgt accagatgga aaaaatcaca
      781 acaggtgtgt catacaccac gtcagcggtg ggaacgggct actggttcct gcagttgctg
      841 gacagggttt ccccgtctca gtgggcggca ataggtgtgc tggggagtct gctgtttggg
      901 ctgctgacgt acctgacgaa cctgtatttc aaaattaaag aagaccggcg taaggccgcc
      961 cggggagagt gaataatgaa ccatgaagaa atgaatcagc gcttcagtcg cctggaaaat
     1021 gaaattgctg aactgaataa aaaactgtcg acgctgatgc cttctgaaga tgaaaaaaaa
     1081 cgccgcgatg agcagtttgc tgcgtttgac gattattgtc ggaaagtgat gagcagaaat
     1141 ctcgcagagt gtttcagtat tcataatgat aatttcagtg
//
LOCUS       DAZGBC010000459         1170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGBC010000459 DAZGBC010000000
VERSION     DAZGBC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.463"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(134..397)
                     /locus_tag="ACHK1J_005405"
     CDS             complement(134..397)
                     /locus_tag="ACHK1J_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO7914510.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            587..829
                     /locus_tag="ACHK1J_005406"
     CDS             587..829
                     /locus_tag="ACHK1J_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914511.1"
                     /translation="MNNESEKAMWRCKPVDNPWTLDSLLKKTISAVLPASDKVSHQNK
                     NKLRNNEFIKIEHPCSKSESRIDGDYIISGGTEYRL"
     gene            complement(877..>1170)
                     /locus_tag="ACHK1J_005407"
     CDS             complement(877..>1170)
                     /locus_tag="ACHK1J_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO7914512.1"
                     /translation="FWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVM
                     GTMFLVLPAVWLGALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
BASE COUNT          345 a          283 c          228 g          314 t
ORIGIN      
        1 atagtgcgct gggttatcgc tctccacggg aatatctgcg gcggcgggca gtaatgggtt
       61 aagtgatacc agatgtctgg aaatatagga gcaaatccac cacgcatgga gcctgttaag
      121 actctcactc tcatcattct cccttctgag tcccaacgaa acgaccaaaa cgctgagtaa
      181 agcgggcaac attgccttct gatgccaccg ttctgagttt accggtatag aacggatgcg
      241 atttggagga aacgtcaatc gtcacatacg gatacgtcac accatccagt tcaatctcgc
      301 gatctgtttt gatggtagaa cctattttaa agtactcatc cacactcgtg tcatggaaca
      361 ctactgtgcg gtattcagga tggatatttg gtttcatctc tcttcactca tgttataaca
      421 taacaataag atacattctc agctagatca tttcaacaga atccccgagc ccaagggtaa
      481 tgtggcatcg cagcctctca tcaaatggaa gacatgtgat tgcacattta tcatacaaga
      541 cgtatgataa cactgtgtta tttatcctgc tgccgagggt atagatatga ataacgaatc
      601 tgaaaaagca atgtggcgtt gtaagcctgt ggataatcca tggacgttgg attcattact
      661 aaaaaagact atttctgctg ttttacctgc atcagacaaa gtttcacacc aaaataaaaa
      721 caaattacgt aataatgaat tcattaaaat tgagcatcca tgctcaaagt ctgaatccag
      781 aatcgatggt gactacatca ttagtggagg aacagaatac cgtttgtaat taagcaggag
      841 aattttctgt aaattctcct gtaacttttt tacttcctat tttatacctc gattagcaat
      901 acttcctgca ctcgaagctg cacctttcgc ttccgaaata ccattcggta atcctcgact
      961 gattccatcg ccaatatgaa caccagccca ggacaacgct cccagccaca ctgccggcaa
     1021 gacgagaaac atcgtcccca tcaccagatt cattatcaga tcatccgatg tgttctggaa
     1081 tcccatcata ttgaaccggg tatgtgtatc cgaactgtac agcgccgtca gcatccagct
     1141 gtcaagccac cgtgcaagct cccaccagaa
//
LOCUS       DAZGBC010000460         1160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGBC010000460 DAZGBC010000000
VERSION     DAZGBC010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.464"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1159
                     /gene="ygeH"
                     /locus_tag="ACHK1J_005408"
     CDS             <1..1159
                     /gene="ygeH"
                     /locus_tag="ACHK1J_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO7914513.1"
                     /translation="CIYSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINEDNTSD
                     YSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNSFLS
                     RFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDELHNTSQFIIDNILQT
                     VHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYEFTPESIYRAMTIFDGLQNKSDIQ
                     TLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSNITNVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNMKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          424 a          212 c          194 g          330 t
ORIGIN      
        1 atgtatctat tctttgcgct gcatttttga aaaaattggc tatgatcgct gcatagaaac
       61 aatctaccgg aaaggttatc gtttcagcgg gcaggttttc aaaactaaaa ttaatgaaga
      121 taatacttca gactattcca tagctatatt ccctttcacc acttcattga atacactgga
      181 tccattaata cttaatcagg aattagtaca aatcatttca aataaaaaaa tcgatggtct
      241 ctatacctat ccgatggctg cgacaaattt ttgtaatgac cacatatctc aaaattcatt
      301 cttgagcaga ttcaagccag attatttcgt tacaggaaga ataaaccaga ataatgcagt
      361 gaacacttta tacattgagt tgatcgacgc taaaaacctt ttcctcatcg ccagtaatca
      421 tctccctgtt gatgaactac ataatacatc acaatttatt atagataata tccttcaaac
      481 ggtacataaa ccagaacgat ctgtaagatt agctaagcag gaccaaggat ataagaatca
      541 ttatttatca gatgaaatgt tagccggaaa gaaagaactt tacgagttca cccctgaaag
      601 catttacagg gccatgacta tatttgatgg attacaaaat aaaagtgata tacagacgct
      661 aaaaacagaa tgttattgcc ttctagcgga atgccatatg tctttggcac ttcatggaaa
      721 aagtgaactt gaacttgctg ctcaaaaagc attagagctt ttagattatg tatcaaacat
      781 aaccaatgtc gatggaaaaa ttttagctat tatgggactg ataactggcc tgtctggaca
      841 agcaaaagta tctcatatct tatttgaaca ggctaagata cactcaactg atatagcctc
      901 tctctactac tatagggcac ttgtcaactt tcataatgaa aaaattgaag aggcaaggat
      961 ttgtatagac aaatcactac aactcgaacc cagaagacga aaagcagttg tgataaaaga
     1021 atgtgtagat atgtatgtgc ctaacccgct caaaaacaac atgaaactct actataaaga
     1081 aactgagagt gaaagccatc gagttataat tgacaacatt ttgaaactaa agcagctgac
     1141 gagaatttgt atgcgataaa
//
LOCUS       DAZGBC010000461         1158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGBC010000461 DAZGBC010000000
VERSION     DAZGBC010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.465"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="ACHK1J_005409"
     CDS             complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="ACHK1J_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO7914514.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          396 a          260 c          222 g          280 t
ORIGIN      
        1 taaaatgtaa tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga
       61 ttgccagctt tcttttaacg cattgaaatt acctgagtaa ataacacatt catgtcttca
      121 ggtttttata tagcgtctgg agacacggat atttatgcaa tgtaatcaac gccagttgca
      181 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
      241 tgttcacttt cccacagcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat
      301 gataatgttt gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag
      361 gttgaaataa tcactagcag gtaattattt caatgatagt gcgcaattga tctacaactc
      421 cgcgtagcgg agagagtatt aatcggatca tagtcacatc aagtgactat gatccgggtg
      481 acaaccggga taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg
      541 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg
      601 ttgcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
      661 agctgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac
      721 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga
      781 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
      841 aattatgact gcggttgctg aataacccga caataagcgc aatacccgag cagattaata
      901 atgcacccac tactgtgctt aaaatatcgc cagagacgaa cgggaaactg atacacagca
      961 acccgacgat aaacagcagc acggcaataa actggattgc tctgcgatgt tttttaagca
     1021 tctccagatc aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata
     1081 aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt
     1141 cgatttaata aaaatttc
//
LOCUS       DAZGBC010000462         1154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGBC010000462 DAZGBC010000000
VERSION     DAZGBC010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.466"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..729
                     /locus_tag="ACHK1J_005410"
     CDS             <1..729
                     /locus_tag="ACHK1J_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7914515.1"
                     /translation="LNDPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEG
                     EQFDTVEVDFGRSEGNNIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDG
                     TVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAE
                     DGTEKRYLPEGTLVLGNTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPARE
                     FTMTQSAPLMVLPDPDEFVVVQVK"
     gene            781..>1154
                     /gene="gpFI"
                     /locus_tag="ACHK1J_005411"
     CDS             781..>1154
                     /gene="gpFI"
                     /locus_tag="ACHK1J_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7914516.1"
                     /translation="MATKEENLNRLRQLAGLLGREVDMSGSAADIALRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEQLNNTDSPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTA"
BASE COUNT          262 a          258 c          375 g          259 t
ORIGIN      
        1 ctgaacgacc cggcctaccg tcgtctgcgt atcatcactg ataacctcaa acaggaagag
       61 catgccattg tccaggtgga ggaaatgcag gcggtgaatg ccgtgctgta tggcaaatac
      121 accatggaag gggagcagtt tgatactgtc gaggtggatt tcgggcgctc tgaaggaaat
      181 aacattgagc aggctgacgg taaaaaatgg tctgagcagg accgtgatac gtttgatccg
      241 acgcatgata ttgacctcta ctgcgatcag gccagcggtc ttgtgaatat cgccattatg
      301 gacggtacgg tctggcgtct gctgaatggc tttaagttgt tccgcgaaaa actggatacc
      361 cgtcgcggct caaattcaca actcgaaacg gcagtgaaag atctgggcgc agtggtgtcc
      421 ttcaaggggt attacggcga tctggccatt gtggtggcga aaacgtctta tgtggcagag
      481 gacggtaccg aaaaacgtta tctgccggag ggcacgctgg tcctgggaaa tacgtcagcg
      541 gagggcatcc gttgctatgg tgccattcag gatgcgcagg cgttgtccga aggtgtggtg
      601 gcctcttccc gttatccgaa acactggatg accgtgggcg atccggcccg tgaattcacc
      661 atgacgcagt ccgcgccgct gatggtgctg ccggatccgg atgagtttgt ggtggtacag
      721 gtgaaataat ccgggagcgg gggcgaaatg cccccgtgtc ttttttcaca gggggctgag
      781 atggcaacaa aagaagaaaa tctgaatcgt cttcgtcaac tggctggtct gctggggcgc
      841 gaggtggata tgtcggggag tgctgcggat attgctctgc gtgtgtctga gtgggaagag
      901 gagcttgcag cttcccggga gggcattatg cactctgatg agagcgggtc tgatcaaaat
      961 gacacagacg atggtgagca gttgaacaac acggattctc cggatgatgt taaagccgtc
     1021 cgggtacgga agtgcctgca agtaatgggg tattgcccgg agacaggtcg tcccgttgag
     1081 ctggcgttac ggggtatgcg tgttctggtg ccatcatcac tggcaacggc catgatacag
     1141 cacggaacgg ctga
//
LOCUS       DAZGBC010000463         1153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGBC010000463 DAZGBC010000000
VERSION     DAZGBC010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.467"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1148
                     /locus_tag="ACHK1J_005412"
     rRNA            <1..1148
                     /locus_tag="ACHK1J_005412"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          279 a          263 c          357 g          254 t
ORIGIN      
        1 ataccagctg gctgcaactg tttattaaaa acacagcact gtgcaaacac gaaagtggac
       61 gtatacggtg tgacgcctgc ccggtgccgg aaggttaatt gatggggtca gcgcaagcga
      121 agctcttgat cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga
      181 aattccttgt cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc
      241 cacccgagac tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg
      301 gaaagacccc gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg
      361 ataggtggga ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca
      421 ccctttaatg tttgatgttc taacgttgac ccgtgatccg ggttgcggac agtgtctggt
      481 gggtagtttg actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct
      541 aatcctggtc ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt
      601 gacggcgcga gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc
      661 catcgctcaa cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca
      721 tatcgacggc ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg
      781 tcccaagggt atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt
      841 gagacagttc ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt
      901 acgagaggac cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc
      961 cggtagctaa atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga
     1021 gatgagttct ccctgactcc ttgagagtcc tgaaggaacg ttgaagacga cgacgttgat
     1081 aggccgggtg tgtaagcgca gcgatgcgtt gagctaaccg gtactaatga accgtgaggc
     1141 ttaaccttac aac
//
LOCUS       DAZGBC010000464         1153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGBC010000464 DAZGBC010000000
VERSION     DAZGBC010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.468"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..1028
                     /locus_tag="ACHK1J_005413"
     CDS             48..1028
                     /locus_tag="ACHK1J_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HFO7914517.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
BASE COUNT          321 a          304 c          307 g          221 t
ORIGIN      
        1 gatatgagat catgtttgtc atctggagcc atagaacagg gttcatcatg agtcatcaac
       61 ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct
      121 tgtcccgcat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt
      181 accccaaggc tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact
      241 gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg
      301 cctccatgcg tctgtttgcc cggttatccc tggatagcgc cttgccggac cgcaccacca
      361 tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca
      421 tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca
      481 ccatcattga ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc
      541 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
      601 ccaagagtgg cctgacccac agcctggtca ccaccgcggc caacgagcat gacctcaatc
      661 agctgggtaa tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
      721 gggcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc
      781 ccggcaaggt aagaaccttg aaacagcatc cacgcaagaa caaaacggcc atcaacatcg
      841 aatacatgaa agccagcatc cgggccaggg tggagcaccc atttcgcatc atcaagcgac
      901 agttcggctt cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga
      961 tgttattcac gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat
     1021 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
     1081 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
     1141 ccgagaagaa ttt
//
LOCUS       DAZGBC010000465         1149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGBC010000465 DAZGBC010000000
VERSION     DAZGBC010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.469"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            315..>1149
                     /locus_tag="ACHK1J_005414"
     CDS             315..>1149
                     /locus_tag="ACHK1J_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7914518.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TS"
BASE COUNT          295 a          331 c          252 g          271 t
ORIGIN      
        1 agttcttatt ttcccatctt ttattatttg caggcctctg ctcattgttg tcgcatgaaa
       61 aagatttaat attttatttt cagccttcac tctatttcct catttcccaa gaaacatatt
      121 aaaaacggaa aacattacac ataacctgga ggacaaatca tgactttaaa tttccctgcc
      181 ggggaaacta cactattctc agcaaaaaac atcctgacag caatactcac tctgcacaac
      241 ttacacgcat agcgttccca tttgaataca gttgcagccc gcgatttatt ttgtcttccg
      301 gagccacctc tgccatgaat atcaaccgca ttatttttgc cattgaagac tgtatcagta
      361 ccctgtcacg gtattccgtt tccgcattct tccccgatta ctgtgatctg cacaccgtca
      421 tcggtctgga ccagcaggac cgcgaacgcc gcccctggct gaaagccccc tacattgcca
      481 ccacaaaaca ggggtattac ctttccgtct ttgaaatcag cggtgccctg aaagaaatgg
      541 atgaaaaccc ggacctgaca gcccccggca cactggaatc actgattaca gcgctggcag
      601 agcgaatgaa cactgcctat aaaaacatcg gtcacaaaat cagttttgtt ttcgaacgcg
      661 atccggagcg tggaaggaat gaaatcgaag ccatgctctt accccagcga aaatccatcg
      721 cacgcacggg gatcctgctt caggacgttc tggatgaaaa agtcaccacg ctcaccccct
      781 ggcttgtccg ggaacgctgc tggctgaccg tctggagctc tgaagaactg ctgagccgga
      841 ccgatcgcaa ggaccaccag acccgtatca ggaaactcgc tgaacacgct ccgccagccc
      901 gatttgcgca ggatccatgg cgctggacac tttccgcact caaaatacgg catgacgcct
      961 ttctggacac gctggagcag gccctgacac atgattctga tggtctgctg gttcgcctga
     1021 tggatattca tgaagtggga cgggaaatcc gccgtcaact ggagcgcaac agcacacctg
     1081 cagtatggca gcctcatctg cctgaagatg cccgtcctgc cggctggcga cagggcgagg
     1141 atacctctg
//
LOCUS       DAZGBC010000466         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGBC010000466 DAZGBC010000000
VERSION     DAZGBC010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.470"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..359)
                     /gene="gpFI"
                     /locus_tag="ACHK1J_005415"
     CDS             complement(<1..359)
                     /gene="gpFI"
                     /locus_tag="ACHK1J_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7914519.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVAEWEEEVCA
                     SENEIADVDDTVYEQAYRNTGEDALGILERIRLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVRSGMAV"
     gene            complement(412..>1140)
                     /locus_tag="ACHK1J_005416"
     CDS             complement(412..>1140)
                     /locus_tag="ACHK1J_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7914520.1"
                     /translation="LNDPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEG
                     DQFEKIEVDFGRSTKNNIIQGSGKEWSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDG
                     TVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGSVVSFKGYYGDLAIVVAKTSYVAE
                     DGTEKRYLPEGTLVLGNTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPARE
                     FTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          255 a          360 c          249 g          276 t
ORIGIN      
        1 acggccattc cactcctgac catgtctttt gccactgagg ggggcatcag aattactctg
       61 ccagcatcaa catgctcaac tggctcacct gtttcatcgt caacgccgca caggtaaaaa
      121 cacttcagaa gcctgatacg ttccagaata cctaaggcat cctcaccggt gttcctgtat
      181 gcctgctcat aaacggtatc atcaacatct gcgatttcat tttccgatgc gcaaacctcc
      241 tcttcccact ctgccacacg ctgtgcgata tccgctgcac tccccgacat atccgcctcg
      301 cgccccagca ggccagccag ttcacgaaga cgtttctgat tttcttcttt tgttgccata
      361 tccagccccc tgtgaaaaaa gacacggggg catttcgccc ccgctcacgg attatttcac
      421 ctgtaccacc acaaactcat ccggatccgg cagcaccatc agcggagcgg actgcgtcat
      481 ggtgaattca cgggccggat cgcccacggt catccagtgt ttcggataac gggaagaggc
      541 caccacacct tcggacaacg cctgcgcatc ctgaatggca ccatagcaac ggatgccctc
      601 agcagccgta tttcccagga ccagcgtgcc ctccggcaga taacgttttt cggtaccgtc
      661 ctctgccaca taagacgttt tcgccaccac aatggccaga tcgccgtaat accccttgaa
      721 agacaccacc gaccccaggt ctttcactgc cgtttcgagt tgtgaatttg agccgcgacg
      781 ggtatccagt ttttcgcgga acagcttaaa gccattcagc agacgccaga cggtaccgtc
      841 catgatggca atattcacaa gaccgctggc ctgatcacag aaaaggtcga tatcatatgt
      901 cgggtcgaac gtgtcacggt cctgttttga ccactcctta ccgctaccct gtatgatgtt
      961 attcttcgtc gatctgccaa aatcaacctc aattttctcg aactggtctc cttccatggt
     1021 gtatttgcca tacagcacgg cattcaccgc ctgcatttcc tccacctgga caatggcgtg
     1081 ctcttcctgt ttgaggttat cggtgatgat acgcagacga cggtaggtcg ggtcgttcag
//
LOCUS       DAZGBC010000467         1139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGBC010000467 DAZGBC010000000
VERSION     DAZGBC010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.471"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(17..433)
                     /locus_tag="ACHK1J_005417"
     CDS             complement(17..433)
                     /locus_tag="ACHK1J_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO7914521.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCARLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            complement(433..687)
                     /locus_tag="ACHK1J_005418"
     CDS             complement(433..687)
                     /locus_tag="ACHK1J_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFO7914522.1"
                     /translation="MKITTLSSRELNQDVTRAKRATSYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
BASE COUNT          338 a          247 c          237 g          317 t
ORIGIN      
        1 aagaagggaa gcgatatcaa gcccagggat taataattga tactccggtt aattcaaaat
       61 caccaacatt tctggttaca accgtcatcc catgtaccag agccgttgct gcgatcaatg
      121 cgtctcgttc actgcgcctg tcaggaacat gtaaacgagc acaacgtaat gcaaccgcag
      181 tatctacagg aagtatctta tcagaaaacg ctggtaacac atgctgatcc atccaggaac
      241 gtagcataat cccttgtcgc atatctcgac gttcaacttg taatattccc tgttcaagtt
      301 ccattatgga aataacagaa ataaagaaat ctgccgcatc tgtatgcaat gcccattttg
      361 caacattatt atctatacgt cctgtccgag ccttccgtag ctcagagata acattagtat
      421 caagaatata cactataaaa actccgcagg ttttaaacta accgtaatac gaggcggttc
      481 aaattcaact gcatcagcat cgggcatcgc cagaatgtca atgagagttc gtcgttgttg
      541 cgtcaatttt cggtattctt cgatactaag aagtacatgt gcggttttac cccgatcagt
      601 aataaatacg ggaccataac ttgtggcgcg tttggcccgg gttacatcct gattcagttc
      661 ccgactggat agagttgtaa tcttcatgct gttgcctttt gattcatata ggtatgttac
      721 tacattgtgg cccatgaaat agtacaatgc aagaaatgta cttgattagg ggtagtcttc
      781 gccgcttgtt gctgtcagat attaatgcta atgtatgcat acattagcat taatggccac
      841 gtcctggatg aggttctacg ctggaacttc atcggataat ctaaactgaa agtctgttgg
      901 ctaaaaaaca gtcttaacgt ggtttatttt ccgaattcca agcggacccc atttaagact
      961 taatacagta ataatgtgta ttattacaca ttattatcca tcaggaagca cgtcgtcagt
     1021 attggagaaa acttacaaac gctcgccgca gccccagcga ccgaacgtta gtgagcgagt
     1081 gggcgaggaa gcggaagagt gtcaggtctg aattgagcac atacgccaga aattatata
//
LOCUS       DAZGBC010000468         1138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGBC010000468 DAZGBC010000000
VERSION     DAZGBC010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.472"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1138
                     /locus_tag="ACHK1J_005419"
     CDS             <1..>1138
                     /locus_tag="ACHK1J_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFO7914523.1"
                     /translation="VAWDPEKKLWYAEAGTDLSLLKRWLPRPQEMSMDAGDPVTEFRQ
                     VLENAGLVLEKDPEMDGEIHRVPTRDDKKGAKSGAYKAYLDGRPSGWYRDYRSADNSP
                     TTWTFSRAENIDPLARLHLKASAQQNRDEKERKLQQQYNKQAGYARSYLNGLPQATAH
                     EYLTRKGIRAAPGVRLNNKNELVIPFSNGRGEIRSYQRIPVTGGKDARILKGSEKSGN
                     WFAFGTPENGRPLLFAEGYATAASLHEATGLPVLMTIDADNMINVAKNARKIWTDSPF
                     VFCADNDHQRKINKGVSSAKKAAASTHGEVIIPAFTEAEKAQGLTDFNDLDASRGRDA
                     FQRAMNAQLEYIGIPTPNNDTTDRREAIVIGNLIFTPVKDEKPQV"
BASE COUNT          318 a          313 c          295 g          212 t
ORIGIN      
        1 gtagcatggg atccagagaa aaaactctgg tatgccgagg cgggtaccga cctttcactg
       61 ctgaagcgct ggttaccccg tccacaggaa atgtcgatgg atgccggcga tccggtcaca
      121 gaatttcgtc aggttctgga aaacgccggg ctggttcttg agaaagatcc agaaatggac
      181 ggagaaatac acagagtgcc cacacgggat gacaaaaaag gggccaaaag tggcgcatac
      241 aaggcctatc ttgatggccg cccctccggc tggtaccgcg attaccggag cgctgataac
      301 agtcccacaa cctggacatt ctcccgcgca gaaaatatcg atcctctcgc cagactgcat
      361 ctgaaggcat ccgctcagca aaatcgcgat gagaaagaac gtaaactaca gcaacagtac
      421 aacaaacagg ccggctatgc ccgctcctat cttaacgggt tgccccaggc aacagcacat
      481 gaatacctga cacgcaaggg catccgggcc gcgcctggcg tgcggctgaa caataaaaat
      541 gagctggtta ttcccttcag caacggccgg ggggaaatac gttcgtacca gcgtattccg
      601 gtcaccggcg ggaaggatgc ccgcattctg aaaggctctg agaaaagcgg caactggttc
      661 gccttcggaa ccccggaaaa tggccgtccc ctgttatttg cggaaggtta cgctactgcc
      721 gcctccctgc acgaagcaac cggccttcct gttctgatga caattgatgc cgacaatatg
      781 attaatgtgg caaaaaatgc ccgcaaaatc tggactgaca gcccctttgt cttctgtgcc
      841 gacaacgacc atcaacggaa aattaataaa ggtgtttcaa gtgcaaaaaa agctgccgca
      901 agcacgcatg gcgaagtcat tattccggcc tttacggagg cggaaaaagc acagggactg
      961 accgatttca atgacctgga cgccagccgg ggacgagacg cctttcagcg agccatgaac
     1021 gcacagttag aatatatcgg tatccccacg cccaacaatg acactactga ccgccgtgaa
     1081 gctatcgtga tcggcaacct gatattcaca ccggtaaaag atgagaagcc acaagtgt
//
LOCUS       DAZGBC010000469         1138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGBC010000469 DAZGBC010000000
VERSION     DAZGBC010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.473"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1138
                     /locus_tag="ACHK1J_005420"
     CDS             <1..>1138
                     /locus_tag="ACHK1J_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFO7914524.1"
                     /translation="VAWDPEKKLWYARAGTELSLLERWLPRPQELSMDAGDPVTEFAQ
                     VLENAGLVIQGLPQMDGAIHRVATRDDKKGAKSGAYKAYLDGRPAGWYRDYRSADDSP
                     TNWVFSGGEQHDPLARLHLRAFAQQQRDDNARKLQQQYNKQARYARSYINQLPQATEH
                     EYLTRKGILAAPGVRLNNKNELVIPFSNSRGEIRSYQRIPVTGGKDARILKDSEKTGN
                     WFTFGTPENGRPLLFAEGYATAASLHEATGLPVLMTVDAGNMIDVAENARQIWTDSPF
                     VFCADNDHQRKINKGVSSAQKAAASTHGEVIIPAFTEAEKAQGLTDFNDLDASRGRDA
                     FQRAMNAQLEYIGIPTPNNDTTDRREAIVIGNLIFTPVKDEKPQV"
BASE COUNT          308 a          324 c          297 g          209 t
ORIGIN      
        1 gtcgcatggg atccagaaaa aaaactctgg tatgcccggg cgggtaccga actttcactg
       61 ctggagcgct ggttaccccg gccgcaggaa ctgtcgatgg atgccggcga tccggtcacc
      121 gaatttgctc aggtcctgga aaacgccgga ctggttattc agggattgcc acagatggat
      181 ggcgccatac acagagtcgc cacccgggat gacaaaaaag gggccaaaag tggcgcatac
      241 aaggcctatc ttgatgggcg ccccgccggc tggtaccgcg actaccggag cgccgatgac
      301 agtcccacaa actgggtatt ttccggcgga gaacaacacg atcctctggc cagactgcat
      361 ctgcgggcat tcgctcagca acagcgcgat gataacgcac gtaaactgca gcaacagtac
      421 aacaaacagg cccggtatgc ccgctcctat attaaccagt taccacaggc aacagagcat
      481 gaatacctga cccgaaaagg gatccttgcc gcaccgggcg tccgtctgaa taacaagaat
      541 gaactcgtca tccctttcag taacagccgg ggagaaatac gttcatacca gcgtattcct
      601 gtcaccggcg ggaaggatgc ccgcattctg aaagactcag agaaaaccgg taactggttc
      661 accttcggaa ccccggaaaa tggccgtccc ctgttatttg cggaaggtta cgcaactgca
      721 gcctccctgc acgaagccac cggccttccg gttctgatga cagttgatgc cggcaatatg
      781 attgacgtgg cagaaaatgc ccgccaaatc tggactgaca gcccctttgt cttctgtgcc
      841 gacaacgacc atcaacggaa aattaacaaa ggtgtttcaa gtgcccaaaa agctgccgca
      901 agcacgcatg gcgaagtcat tattccggcc tttacggagg cggaaaaagc acagggactg
      961 accgatttca atgacctgga cgccagcagg ggacgagacg cctttcagcg agccatgaac
     1021 gcacagttag aatatatcgg tatccccacg cccaacaatg acactactga ccgccgtgaa
     1081 gctatcgtga tcggcaacct gatattcaca ccggtaaaag atgagaagcc acaagtgt
//
LOCUS       DAZGBC010000470         1134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGBC010000470 DAZGBC010000000
VERSION     DAZGBC010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.474"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..859
                     /locus_tag="ACHK1J_005421"
     CDS             38..859
                     /locus_tag="ACHK1J_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO7914525.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVHHPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            949..>1134
                     /locus_tag="ACHK1J_005422"
     CDS             949..>1134
                     /locus_tag="ACHK1J_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO7914526.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGP"
BASE COUNT          309 a          294 c          245 g          286 t
ORIGIN      
        1 ctctttagac ttaacatatg aaccgcacgg aggtgaaatg aaacccaaca gcacaggcat
       61 tgctgcccgc atgatgctct ccattgacag ggcccggatt agcgaagctc tgctcaataa
      121 ccgccagttg cagtccactc ccctgcagat ccgttatccg cagggcgtca gagatgcact
      181 cggaataatg agcgagcaac tctccctttc tgtatccgac ctgacgcgta tcctggtcga
      241 agatgcatta actgaaatgt ttctcccggc tgaaaacatc gttcaccgtc ttatctgccg
      301 gacagaacac atcatgcagg cacatgatgt ctgcgtaaca acgatgacaa cgttactggc
      361 tccctggaat atccgtcctg ccgttttccg tgacccggac aaactcgcag attatctgac
      421 aggagagatt ctgactgctc tggctgactg gttttatctc aacccggaat ggctgaacgg
      481 cagggtacat catcctttaa gccgtgccgg tgagtggcca gcaacacagg atgcgttctg
      541 ccagcttatt tcagccagcg ataatgtgga tattattatc tggcacagtt ttccttttgc
      601 cggtgttcat tcacaggaat attgcggtgt actgttccgc aagaaaaaag aaattaataa
      661 agccatcatt tatcctgtac tttctttatc acctgtaaga atgaataagg aaaaagaaaa
      721 ctggtttcac actgcccgca atttatatgc aaagacatcc gtccggaccg taacgcttcg
      781 tccggcacag gcagaatatc tgatgacagg aaaaatatta cctgctgagt tattcagtat
      841 accgttatta ccctggtaat aacgcagaga aaaaaccagt aaatttttaa tggcacagtc
      901 gtgtcatacc gttgctgtac ccaaaacacc tcaaaccagg aaactgaaat gatgatgaat
      961 atgcacatta cccgaactct catcgcatct gctgttttat tttcctttaa ttcaacagcc
     1021 gcaacttcat attttgaagc ccgcaatgat gcaatggggg gaaccggggt agcctcctct
     1081 cactacggtg tggcacctct ggcaaacccg gccctgctga caaaagccgg tccg
//
LOCUS       DAZGBC010000471         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGBC010000471 DAZGBC010000000
VERSION     DAZGBC010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.475"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..131)
                     /locus_tag="ACHK1J_005423"
     CDS             complement(<1..131)
                     /locus_tag="ACHK1J_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFO7914527.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQK"
     gene            177..962
                     /locus_tag="ACHK1J_005424"
     CDS             177..962
                     /locus_tag="ACHK1J_005424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914528.1"
                     /translation="MRITDNKKTNQAEYTFNAAYFLIAVATGNTAHFLTSVATGNTAH
                     FLISVATGNTAHFLISVATGNTAHFLTSVATGNTAHFLITVATGSTVHFLTSAATGST
                     VRFLTSAATGSTAHFLISAATGSTVRFLISSATGSTVRFLTSAATGSTVHFLTSAATG
                     STAHFLIPAATDSTVHFLTSAATGSTVRFLTSAATGSTAHFLISAATGSTVHFLISVA
                     TGNTAHFLTSVATGNTAHFLITVATGNMVNDDYYLNVQRQKQH"
BASE COUNT          256 a          323 c          203 g          350 t
ORIGIN      
        1 tccttctgat tattcctgcg tccctcccag agacggatcg catatcgggt atgaagggca
       61 atagtcagcg atgactgcag agcaccaact tgttctgatt ttctgacttc tccggttttc
      121 tcctggggca ttgtgtttac tccgaatgat ttcatgacca gaagatagtc cgcaggttgc
      181 gtattaccga caacaaaaaa acaaatcagg cagaatatac atttaacgcc gcttattttt
      241 taatcgctgt tgccactggc aacaccgctc actttttaac ctctgttgcc actggcaaca
      301 ccgctcactt tttaatctct gttgccactg gcaacaccgc tcatttttta atctctgttg
      361 ccactggcaa caccgctcac tttttaacct ctgttgccac tggcaacacc gctcattttt
      421 taatcactgt tgccactggc agcactgttc actttttaac ctctgctgcc actggcagca
      481 ctgttcgctt tttaacctct gctgccactg gcagcactgc tcatttttta atctctgctg
      541 ccactggcag cactgttcgt tttttaatct cttctgccac tggcagcact gttcgctttt
      601 taacctctgc tgccactggc agcactgttc actttttgac ctctgctgcc actggcagca
      661 ctgctcattt tttaatccct gctgccactg acagcactgt tcacttttta acctctgctg
      721 ccactggcag cactgttcgc tttttaacct ctgctgccac tggcagcact gctcattttt
      781 taatctctgc tgccactggc agcactgttc attttttaat ctctgttgcc actggcaaca
      841 ccgctcactt tttaacctct gttgccactg gcaacaccgc tcatttttta atcactgttg
      901 ccactggcaa catggttaat gatgactact accttaacgt tcagagacaa aaacagcatt
      961 aatgtcgtta tccccatcac gagcaactga atgtcggtta tatccagatg agacaatgac
     1021 cagaaaatca caaaaaatga aaagcattct gccagcagac tcaggaggag cataaattgc
     1081 aggccccctc cgactgtact gcctgtacgc gctttataaa gactgtatgt ga
//
LOCUS       DAZGBC010000472         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGBC010000472 DAZGBC010000000
VERSION     DAZGBC010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.476"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1129
                     /locus_tag="ACHK1J_005425"
     rRNA            <1..>1129
                     /locus_tag="ACHK1J_005425"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          310 a          239 c          360 g          220 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 atgtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1081 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaagg
//
LOCUS       DAZGBC010000473         1122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGBC010000473 DAZGBC010000000
VERSION     DAZGBC010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.477"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..641)
                     /locus_tag="ACHK1J_005426"
     CDS             complement(<1..641)
                     /locus_tag="ACHK1J_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO7914529.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKT"
     gene            838..>1122
                     /locus_tag="ACHK1J_005427"
     CDS             838..>1122
                     /locus_tag="ACHK1J_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914530.1"
                     /translation="MNTEKQLPSGIESEVKYTLQFWLLSNGKVMTEKDGLCNPFPLNC
                     IESAHPFTPFQQNQWVELDTVGPNGPVKATFLVHSVLYKLKRQGGSLNDAG"
BASE COUNT          275 a          316 c          292 g          239 t
ORIGIN      
        1 gttttcccca ttgacggacg ggcagccagc agaaccaggt caccaggctg caggccacag
       61 gtcatttcat ccagacagtc aaatcccgtg ggggtaccgg taacaccacc ggagccgagc
      121 acactttcca gttgctgcag aacggtatca agcgcggatg aaagagaggc ttccgtgcgt
      181 tcccggagcg tcctgttctg cgcaagtgca aacagacgcc gctctgcagc atccagcaca
      241 tcccgtgatg tggcgttcgg tgccgccaca tcagcaatca gggtgtttcc ggtggcctgt
      301 aactgacgca gaacgctttt ctccaccaca atgcctgcat aggccatgat gttggccgca
      361 gaaggtgtgt tcttgctgag ctcggccaga taagcaaagc cgcccagtac gtccagcctg
      421 ccctgatgtt ccaggtgctc tgacagtgta atcaggtcca gtggctggcc attcaccgcc
      481 agttcagaca tggcacggaa aatagcccgg tgctgagcca cataaaaatc gtcaggactc
      541 agcatcagca ccacttcatc ccagcgttca ttctccagca tcagaccgcc cagaacggcc
      601 tgctctgctt cgtatgcgca gagaacagac atttcagcca tggcgccccc tccccctctg
      661 acagccggaa acctggctac gtgaaaaaag gaaaaagggt gccggacgta cagctgtcgg
      721 gaataaaatc ccggtaactg tctgtacgtt ttcagaccgg tgagtttgcc gcccccggtc
      781 tgaaggtacg gacagcattc gttcattcat aacaagttgt tttaaaggaa aaagtttatg
      841 aatacagaaa aacaactgcc gtccggcatt gaatctgaag tgaaatacac cctgcagttc
      901 tggctgctga gtaacggaaa ggtcatgacg gaaaaagacg ggctctgtaa cccctttccc
      961 ctgaactgta ttgaatcggc tcatcctttt actccgtttc agcagaatca gtgggttgaa
     1021 ctggacactg tcgggccgaa tggaccggtg aaagcgacat tccttgtgca cagcgttctg
     1081 tataaactga aacgtcaggg tggctccctg aatgatgccg gc
//
LOCUS       DAZGBC010000474         1121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGBC010000474 DAZGBC010000000
VERSION     DAZGBC010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.478"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..755
                     /locus_tag="ACHK1J_005428"
     CDS             63..755
                     /locus_tag="ACHK1J_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7914531.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQLGKPTANHPKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            836..>1121
                     /locus_tag="ACHK1J_005429"
     CDS             836..>1121
                     /locus_tag="ACHK1J_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7914532.1"
                     /translation="MDTLKYEKFSDFDHNDPFFDSLKKDYKEFPLWLEKKAREGESAY
                     VLYDDKHKIEGFMYLKENDDANDINPALPPGRHLKIGTFKFESKGTLRGQR"
BASE COUNT          366 a          219 c          248 g          288 t
ORIGIN      
        1 taatcataac aatagcaaaa cgtgttttga ccatcaatac gcaagcgtat aaattaaaac
       61 ttatgaaaaa agaaactctt gctgatcgct taaacctagc gatggaacaa tctggaatgt
      121 ctcaaggcgc tcttgcaaag gcgtctggcg tagctcaacc cacaatctgg agactgacaa
      181 gcggcaacgc gcgcggctca acaaaaattg ttgaaatagc taatgcattg ggtgttcgaa
      241 cagagtggct ctcatcaggc ataggcccga tgagaaatga cggtcaacaa ttagggaagc
      301 ctactgccaa ccatcccaaa tacttcaaga ttgacgttct tgatatagaa gtgagtgccg
      361 ggccgggagt catcaaccgt gagtttgtag aagttctacg ctcggttgag tactcgtttg
      421 acgatgctcg tcacatgttc gatggtagga aggcagaaaa tatccgcatc attaacgtac
      481 gcggtgacag catgtcagga acgattgaac ctggtgatct tctattcgtt gatatcacgg
      541 ttaaatcttt cgacggtgat ggtatctatg cgtttctgta cgacgacaca gcccatgtaa
      601 agcgcctgca aatgatgaag gataagctgc tggttatctc tgataacaaa agctactcac
      661 cgtgggaccc gatcgagaaa gatgagatga accgggtatt tatcttcggg aaagttattg
      721 ggagcatgcc gcagacgtat aggaagcatg gatagtacca attaaaaatt atcaaccggg
      781 cattgtgctc attcagtaaa acaacttaat tattcatttt agaatggaga acttaatgga
      841 tactttaaaa tatgagaaat tctctgactt tgatcacaat gacccatttt ttgactcttt
      901 aaaaaaagat tataaagagt ttcctctttg gttagaaaaa aaagccagag aaggagaatc
      961 agcttatgtg ctctatgatg acaagcataa aatcgaaggt tttatgtatc taaaagaaaa
     1021 tgatgatgca aatgacatta atccagcgct cccaccagga cgtcatctaa agataggaac
     1081 attcaaattt gaatctaaag gcacccttcg cggacaacga t
//
LOCUS       DAZGBC010000475         1107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGBC010000475 DAZGBC010000000
VERSION     DAZGBC010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.479"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(103..1047)
                     /gene="leuO"
                     /locus_tag="ACHK1J_005430"
     CDS             complement(103..1047)
                     /gene="leuO"
                     /locus_tag="ACHK1J_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO7914533.1"
                     /translation="MPEVQTDHPEMAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          322 a          266 c          223 g          296 t
ORIGIN      
        1 taatcctaaa atagagagcc cacatattca gaataaaatt ctacattcaa taaaaaatgc
       61 agaataaacc agacattcat gtctgaccta ttctgcattc agttagcgtt tgcaaattga
      121 gactaattgc tcttccatcc actgatggcc tttatcgcgc ccggcagctt catgccagga
      181 gagataacag gttctgctat ttagttttaa cggcagcggt aatacctgta attctaagga
      241 ttcagcgaac tcttcagcca gccaacgcgg cgcaatagcg accaaatgcg tttgcgacac
      301 cacgctaagt acgctcatca ttgccatgcc ctgatacgcg atactggctt gcttatctac
      361 cgtgtcatac cacggttgac taaatgacgc gaaacgatcg agcgaaaccg ccgcatgttg
      421 ttcgttataa acatcatgtt tcagtaacgg gcccttaatt gttgggtgat ttttgctggc
      481 taccagcacc atttcatctt taaataatgg cacgctggta aattcaggac gatggaagtc
      541 ttcataacta atcacaaact ccgtttcctg ataacgcagc tgatgttcag tgttctgatt
      601 taatgaagac ttgaacataa catgtatatt tggcgcaatc tgctcaatgt gattataaat
      661 ctgcgaggtc agaatactat ctaacgggct gcaaacacaa agatgaaata cacgttcact
      721 gctcgcgggt tcaaaacctg aaccaggcaa ttcattttgt actagttgca atgcctgacg
      781 aactgaacca aaaagttgaa atgcgcgagc agtcggttga ataccacggc cataacgaac
      841 aaaaagctcg tcattaaaca tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg
      901 cgacattccc agaacatgag cagcacgggt aatgttttgc tcctgcatca cggcatcgaa
      961 aacggttaat aagttgagat cgaccatgcg tagctgtggc ttgctgaact ccgccatctc
     1021 tggatgatct gtttgtacct ctggcatact taactccact gtcacactta actccctttc
     1081 ccttattgga atgcagagaa ataatcc
//
LOCUS       DAZGBC010000476         1105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGBC010000476 DAZGBC010000000
VERSION     DAZGBC010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.480"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..75
                     /locus_tag="ACHK1J_005431"
                     /pseudo
     CDS             1..75
                     /locus_tag="ACHK1J_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            373..720
                     /locus_tag="ACHK1J_005432"
     CDS             373..720
                     /locus_tag="ACHK1J_005432"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO7914534.1"
                     /translation="MFTQHIEKQRNVHTLNQNDINNSVNSVNVPENELTYQFQRWGQN
                     HTVRILESSEGIRLKPSDALVSDRLHEAQNNDVTAQRWVLTEQDERQGQRHQPHEEQE
                     NEGKFENDQKDES"
     gene            680..1105
                     /locus_tag="ACHK1J_005433"
                     /pseudo
     CDS             680..1105
                     /locus_tag="ACHK1J_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          405 a          194 c          235 g          271 t
ORIGIN      
        1 actcaggata gccaattttc tacgttgggg aaaaaaaatg ggcaaatgaa gtctattgac
       61 aacaaatcat ctctccaggt tgacaggaat ccctctcttt ctttgcaaac aaatggaaca
      121 gagcaatttt ctacaaagaa aattctcaat atgacttctc gaggtcaggg tattagttct
      181 gaactatctg ataacacgat aaaaattaag gaaaaaatcc ataatggtag ccatacagag
      241 tacatcacag atcaaaaaaa cagtaataat aaagatcgtg aaaaaaaata ccgcgatgga
      301 gacaaaataa atggaccgca ggctcattca ttagacagta ctaacgaaag aaggtttgct
      361 gataacagaa ccatgtttac tcaacatatc gaaaagcaaa ggaatgttca tacgctcaat
      421 cagaatgata taaataattc cgttaatagt gtcaatgtgc cagaaaatga attgacatat
      481 cagtttcagc gttggggaca gaatcataca gtccggattc tggaaagtag tgaagggata
      541 cgactaaaac catcagacgc gcttgtctcc gacagactcc atgaagccca aaacaatgat
      601 gttacagcac aacgttgggt attgactgag caagatgaac gacaaggaca acgacatcag
      661 cctcatgagg agcaagaaaa tgaaggcaaa ttcgaaaacg atcagaagga tgaaagttaa
      721 tgttcggtct gaggaaagta aatcgaaaca ctcatacgtt tgaaaccact tttcaaaatt
      781 ggaaagaaaa tggcgaagat gttgcattat taatgcccga atttagtgct aaatggctac
      841 caatagcaga agagagtggc agctggtctg gatgggtatt gcttcgagaa atatttccct
      901 taatttcttc tgagctcgct gggatggctt taatgccaga aacggaaagg ttaatagggg
      961 aatggctcag tttatccagt tccccgttaa atctaaagca tcctgaacta aaatataatc
     1021 gtttgtgcgt aggtaaagta tttgatggag tattgagccc agctcaacca ctaataagaa
     1081 tatggacagg ggaattgaat ctttg
//
LOCUS       DAZGBC010000477         1104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGBC010000477 DAZGBC010000000
VERSION     DAZGBC010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.481"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..135
                     /locus_tag="ACHK1J_005434"
     CDS             <1..135
                     /locus_tag="ACHK1J_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin subunit A"
                     /protein_id="HFO7914535.1"
                     /translation="EEIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE
                     "
     gene            148..411
                     /gene="stxB2b"
                     /locus_tag="ACHK1J_005435"
     CDS             148..411
                     /gene="stxB2b"
                     /locus_tag="ACHK1J_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO7914536.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            934..>1104
                     /locus_tag="ACHK1J_005436"
     CDS             934..>1104
                     /locus_tag="ACHK1J_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7914537.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPV"
BASE COUNT          309 a          239 c          276 g          280 t
ORIGIN      
        1 gaagagatac aaccagaatg tcagataact ggcgacaggc cagttataag gataaacaat
       61 actttatggg aaagtaatac cgcagctgct tttctgaatc gcagggctca ctctttaaat
      121 acatccggag aataacagga gttaaatatg aagaagatat ttgtagcggc tttatttgct
      181 tttgtttctg ttaatgcaat ggcagctgat tgtgcaaaag gtaaaattga gttctctaag
      241 tataatgaga atgatacatt cacagtaaaa gtggccggga aagagtactg gactaaccgc
      301 tggaatctgc aaccgctact gcaaagcgca cagttaacag gaatgacggt aacaatcaaa
      361 tcaaatacct gtgcgtcagg ttcaggattt gctgaagtgc agtttaatta atatcagaag
      421 tattgctggt ttcgtggtgt gcagcaatgt agttacagtg caatcaatgt cacaattcag
      481 tcagttgaca gttgcctgtc tggctgagca tttgttaaaa aaatcctgca tgatgaatcc
      541 ccctgagcgg aggggcataa tgacagatgt ttggttgcgt attgtatagg caagttgcgg
      601 attctgtctg gtcattgcag aattcaccgg gaggcacccg gcatcatgct gtatacagag
      661 attaggcata tatccaggct cctcatcgca ggagcctttt tacatgcaaa aaaagcccgc
      721 gccgggagac gcgggcagca aggaataaac aacaaaacgt gaagtaatca atttttcagc
      781 tggcgaataa tacccgacag taatcactct gcgcaactgc gcggcctttt tcgtattgcg
      841 ggctgtagtc ttccttctgt cattgtcctg taacttccgg acttcagccc gccccttatc
      901 tgactcacaa cattatcccg gccgggagga ttcatggcat ttaaacacta tgacgtggtc
      961 agggcggcgt cgccgtcaga ccttgcggag cgactgactc aaaaactgaa ggaggggtgg
     1021 cagccatttg gcagcccggt ggccatcacg ccttataccc tgatgcaggc cattgcggcg
     1081 gaaggtgatg tcaccacacc tgtg
//
LOCUS       DAZGBC010000478         1098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGBC010000478 DAZGBC010000000
VERSION     DAZGBC010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.482"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1098
                     /locus_tag="ACHK1J_005437"
     CDS             <1..>1098
                     /locus_tag="ACHK1J_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914538.1"
                     /translation="MFLSSLSSLVVVNDPKKMKRIFPLFIVSENINIHAEPDIEYFRV
                     IDRAFDEVANYTGRIFSYLRTHEPLKLTSCVNKQTLLSMGGYLNEWNVFDSLSRVRDF
                     FRLSSAVFTKLDNNIYSLEVDSFCLYRDYEIARNRLMMRASHLYSEVHEFSNKHFHLN
                     SWVKDHMPSYLNSDGVFSSFHLSELENISPDDLHEEYGNISLFNWVHAYQCLVELSKE
                     EMSKRFSSTKPIPLQLDRWLIIKSRESWLSFFQRKGIAADAAKKLIDYFTFNSKSHDL
                     NDCPFIPCMDGLCLMPALIANSSVTRSLMSLFGSKKISQASKGRFHEQQFIKQVRDAG
                     IKASPIDAHANYQCDCVILLDDCLIFTELKS"
BASE COUNT          350 a          174 c          204 g          370 t
ORIGIN      
        1 gatgttttta tcatcattat catccttggt tgtggtcaat gatcctaaaa aaatgaagag
       61 gatttttcca ttatttatcg tttctgaaaa tattaatatt catgctgaac ctgatatcga
      121 atattttcga gttatcgata gggcatttga tgaggtagcc aattacaccg gacgaatatt
      181 ttcttattta cggactcatg agccattgaa actaacttct tgtgtgaata aacaaacctt
      241 gctttcaatg ggagggtacc ttaatgagtg gaatgttttt gactcattaa gcagagtaag
      301 agactttttc agactaagta gtgcagtatt tacaaaattg gataataaca tttactctct
      361 tgaggttgat agtttttgtt tataccgaga ttatgagatt gcaagaaata gactaatgat
      421 gagagcgtca catctatata gcgaagttca tgaatttagt aacaaacatt ttcatctaaa
      481 tagttgggta aaggatcata tgccatctta cttaaattct gatggcgttt ttagctcatt
      541 tcatctaagc gagttggaaa atatatctcc tgatgacctt catgaagaat atgggaatat
      601 atcattattt aattgggtgc acgcttatca atgtttagtc gagttatcta aagaagaaat
      661 gagcaaaaga ttttcatcga caaaacctat acccctacag ttggatcgct ggctgatcat
      721 aaaaagccgt gagagttggt tatcattctt tcaacgtaag gggattgcag cagatgcagc
      781 aaaaaaactg atagattatt ttaccttcaa ttcaaaatca catgatctta atgattgccc
      841 atttattccc tgtatggacg gtttatgctt aatgcctgca ttaatagcaa atagctcagt
      901 gactcgctct ctaatgtcat tatttggttc caaaaaaatt tctcaagcaa gtaaagggcg
      961 atttcatgag caacaattta taaaacaagt tcgtgatgcg gggattaaag cttctccaat
     1021 tgatgctcat gctaattatc agtgcgattg cgtgattctc ctggatgatt gtttgatttt
     1081 tacagaacta aaatcaaa
//
LOCUS       DAZGBC010000479         1090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGBC010000479 DAZGBC010000000
VERSION     DAZGBC010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.483"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            264..509
                     /locus_tag="ACHK1J_005438"
     CDS             264..509
                     /locus_tag="ACHK1J_005438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914539.1"
                     /translation="MEEMQDKMAWMQNVITEKLGPSLWHMHPIAFPGALIKKNTGWAH
                     SKFANLLGSVELENDYSAYNVTKTGKSVPHYNINLTK"
     gene            680..>1090
                     /locus_tag="ACHK1J_005439"
     CDS             680..>1090
                     /locus_tag="ACHK1J_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914540.1"
                     /translation="MGCNKKLNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIHFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGSPVGL"
BASE COUNT          371 a          165 c          231 g          323 t
ORIGIN      
        1 aaaattagca ttcaagatgg taatgactct aaaaattctg agtgaacaac aaattagcga
       61 agttaaaata gttgcgtttc ttatctggtc ggtttgatat ttttgttagt attgccatac
      121 ttgtaaagtt aatcaaatat catcaattga tttagtgtaa tttcaaaata ttaatatatc
      181 aaaatacgat gagttaaacg actttataac gaattcaaaa ctaatgaaaa atgcttaaat
      241 gcactgaaaa aagacgtacc ggaatggaag aaatgcagga taagatggcc tggatgcaga
      301 atgtgatcac ggagaaactg ggcccttcgt tgtggcatat gcatccgatt gcctttcctg
      361 gagcattaat aaagaaaaat actgggtggg cgcatagcaa gtttgcaaac cttttgggaa
      421 gtgtcgagtt agaaaatgat tattcggcat ataatgttac taagacaggt aaatctgtcc
      481 cgcactataa tattaatctc acaaaatgac attatctgcg gtaattgatg ccccaagaga
      541 acgaaaaatg gtaaaaagaa atacaggtta gtacctaggg tggcagaagt tgagggggag
      601 tcgtactatt ctggtgatgg attgaataaa gctcacctat taccagatac gatgattaaa
      661 gctcttgagg attctaaaaa tgggatgcaa taaaaaatta aacaaatttt tgctttttgt
      721 gtcatgcatg ctcttgtcgt ttgtaacgta ctcacaagac agttctaatc ctcagttttc
      781 tgactacctg gttcctgtaa gcaatggacc atttgaaaag aatattcact ttaataaaga
      841 gcaggaaaat tactcgcaac actggaagaa tgcagtccaa gaggaactaa aaaagcctgt
      901 taattttgca gggcacttta gaatctatac agcctttggc ggtcatggta aagaatgcct
      961 tcgtgataat tgggtatgtg gttgggttat tgataaacta tcaggggaag ttgtagcaac
     1021 acttccatca gataataatg gaagttataa ctatgctgat gtgtctgata atggtagccc
     1081 tgttggtctt
//
LOCUS       DAZGBC010000480         1089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGBC010000480 DAZGBC010000000
VERSION     DAZGBC010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.484"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..189
                     /locus_tag="ACHK1J_005440"
     CDS             <1..189
                     /locus_tag="ACHK1J_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO7914541.1"
                     /translation="PQPDRDRRRQIRTRWSLTDDVIPRRHDNDEGSDTGETTETTETT
                     ETTETTETRHPADKEKAQ"
     gene            186..>1089
                     /locus_tag="ACHK1J_005441"
     CDS             186..>1089
                     /locus_tag="ACHK1J_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO7914542.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTEPPGHHIGRMLSAIDSCLSAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSATPPITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNRTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAP"
BASE COUNT          295 a          332 c          259 g          203 t
ORIGIN      
        1 cctcagccag accgcgatcg cagacgtcag ataaggaccc gctggtcact cacggatgac
       61 gtgataccac gccgccatga taacgatgaa ggctcagata ctggtgaaac caccgaaacc
      121 actgaaacca ctgaaaccac tgaaaccact gaaacccggc atcccgccga caaggaaaaa
      181 gcacaatgac catccgctac actgcaacac tgaacaataa acacctgtcc ctgaccgccc
      241 tgtaccctga cggtggtcag gaggttattg ccatcctgcc ggttacagaa ctgggcgcac
      301 tgattaaccg taataacagg ctgatgcacc tgtccttcag cacccctttt cgtgaaataa
      361 ccgaaacact gcttgttgat accgttgata ctgaacctcc cggacatcac atcggcagaa
      421 tgctctccgc gattgacagc tgtctgagcg cggcatatgc agaacttcct gcccggaaaa
      481 ccagcttcat gagagggctg atatacggtg caggattagt gctggcactt ctgacagcgg
      541 taacagccgg atatatttta ctgaaaccgg acagcgattc ccctctcagg gtacctgtca
      601 cagaatccgt aacagaaaca gcaccattac acatcgacaa ggaaattgtc cggtcagtag
      661 ccccgtcaga gacgaaaaat gaaatcgttc acccggcacc acaggaaatg caggagacat
      721 ccgctacccc gccgataaca gcagacaaaa atactctggt cgctgaagcc cgccgtaatc
      781 tggccactgt acttaaacgc aacgcggacc ggggtatgtt caccattaat ctctccactg
      841 gtcatgaacg cacactctac gccttccttg accccgcatg catcaactgc cgtcttctgg
      901 agcccgcgct taagcaactg gcccgggaat tcagcgtggt gatttatccg gtctccgtaa
      961 tcggcggaga ggccagcaca aaccggactg cgccactgct gtgtgaaccg gacgcacaaa
     1021 aacgggcgac cggatggcat cagctgtatt ccgcagacgg tggtatggca cccaccggcg
     1081 atgcaccgg
//
LOCUS       DAZGBC010000481         1084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGBC010000481 DAZGBC010000000
VERSION     DAZGBC010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.485"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            150..392
                     /locus_tag="ACHK1J_005442"
     CDS             150..392
                     /locus_tag="ACHK1J_005442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914543.1"
                     /translation="MMMARDITAYHLNSVHISARRFPEIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            830..1084
                     /locus_tag="ACHK1J_005443"
                     /pseudo
     CDS             830..1084
                     /locus_tag="ACHK1J_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
BASE COUNT          283 a          209 c          254 g          338 t
ORIGIN      
        1 atttattttt tgaaataaat aatgtggtat gataataaat ccaggtcagc agtaactggc
       61 tatcgtgtat aattatccag aagtcctgct gagccttccg tctgttcatc atgtgccagg
      121 tttgcgttcg ggatatgatg tgccatctta tgatgatggc aagagatatt actgcgtacc
      181 acctcaattc tgtacacatc agtgcccgtc gtttccccga gatttttcag tgggccaccg
      241 gtatgcaaac aaaaaatctg ccgttaacta attctttgtt acagaatgat tacgcagaaa
      301 gtatttttgt atttaatggc gaacgtgacg tttcggaaat gcctttaccg gaagaaatgc
      361 tactgactgg aaacgctgag tcagaaagat aattcggtgt attcatgact gttatcaatg
      421 tgttatgtgt atgatgtcaa cgacggatga aaagttatcc acttatatga ggcattatag
      481 gattatttct ggctgacgtt gtttcagttc agaccagggg cagtgaatat gctgacctgc
      541 tttttcgctg atatcagtcc ccctgtaatt acatagataa tagatatacc tctccgttga
      601 tcaggctgtc caggtaattg ataccgcact tcctcatcag ttcgtagcag agtgtactta
      661 gcatggctag tgcagtttct gatgatttac agtgtgtatt caggagagta agaaattgct
      721 caaaacatcc tggtttatac tggtgctttg atattcattg tgtccctttt tcgttcgtta
      781 ttatttccat atttaatatg atgcctgtac atgtaaaatt ggtgttcagg aggtctataa
      841 tgaaatccgg caggggactc ccgtcagtcc ggtcataatg gctggtgatg cctggctcac
      901 gacaaaaatt cgttctcagt tacttacaag tgcccgtgtc aaatcatcca gtgtcaaggt
      961 ggtgacagaa aacagcgaag tctttttact gggcctggtt acagagcagg aagggcgagc
     1021 ggcagcagat gttgccagta gggtgagcgg agtgaagcgg gtcacgacgg ccttctccta
     1081 tatt
//
LOCUS       DAZGBC010000482         1083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGBC010000482 DAZGBC010000000
VERSION     DAZGBC010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.486"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..524
                     /locus_tag="ACHK1J_005444"
     CDS             <1..524
                     /locus_tag="ACHK1J_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7914544.1"
                     /translation="QMNEGWYQIRIAGEDTAPEAVYARLHEQLGEGAVIHIVPRLAGA
                     GGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGLTATTMLFSLGASMILGGVAQ
                     MLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRD
                     EGGDGKVVVIGRG"
BASE COUNT          300 a          213 c          330 g          240 t
ORIGIN      
        1 gtcagatgaa tgaaggctgg tatcagatac gtattgccgg tgaggatacc gcaccggagg
       61 cggtgtacgc ccgtcttcac gaacaactgg gtgagggagc ggtcatccac attgtgccgc
      121 gactggccgg agccggggga aatggtgttt ttcaggtggt gctgggggtt gcagcgattg
      181 tcgggtcatt tttcactggt ggtgcaacac tggcggcatg gggcgcagcc ctgagtgccg
      241 gagggctgac tgccaccacg atgctgttct cactgggtgc cagcatgata ctgggtggtg
      301 tggctcagat gctggccccg aaggcaaaga cgccggagta taaaagtacg gataacggta
      361 aacagaacac gtatttttcg tcactggaca acatgattgc ccagggtaac ccgatgccgg
      421 tgccttacgg tgaaatgctg gttggttcac gacggatatc ccaggatatc agcacccgtg
      481 atgagggcgg agacgggaaa gtggtggtta tcgggcgggg ataaaaataa aaaaaatccc
      541 gcagagtcag cggagctgcg ggaaataacg atgaagatta acgttatgga gttatttttt
      601 tgggcagaaa aacattaacg cagcgaaatt ataagcgcca catggatttt gcgaaaatgt
      661 gaagaaattc agaaattttt tgctgcagag gtgcagggag cttgccgggt atctgtggca
      721 gaagacagaa aacatcatgc cggggttaag ggataaaaaa atcccgcaga gtccggagac
      781 tgcgggacag aaccgatgaa gattaacgtt caaggagttt tgtttttatt gacccgaaaa
      841 aactgtaacg ccccggaatg atatctgcca cgggaacgta cagaaaatgt gaagaaattc
      901 agactttttc tgtcgcaggt ttacagaacg gatgaccgcc agggtaccag tggcgtaaat
      961 gagcaggatg ttgtggtgtg gctgaagcaa aaaaatctcc cgtaatatcg ccggatactg
     1021 acgggagacg aatagtcacc ttaaggaggt ctgataatta ttgcttttat tgagcaaaaa
     1081 aac
//
LOCUS       DAZGBC010000483         1077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGBC010000483 DAZGBC010000000
VERSION     DAZGBC010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.487"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1077)
                     /gene="espX6"
                     /locus_tag="ACHK1J_005445"
     CDS             complement(<1..>1077)
                     /gene="espX6"
                     /locus_tag="ACHK1J_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFO7914545.1"
                     /translation="QQFIKIMEQKAQLSIYIDKNNLKESIFSVLHNDPQHVDKDQHLP
                     CEKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIRDNNNKTETFSFTN
                     LQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEIIECASPEIGSSVLNDLY
                     SIANFLPDNNLTPNESWKRFCDTCERFYVAQKSITGDKSERLTRKLSISDAGITMTFK
                     IGDVVINTIITAIPEDATGQRCIEGLNLAEMDLTGIDLSKMVLRNVNFNGSILRHAKF
                     SGTICEGVDFTDCDLRNAKFKNASLENNDFRKVRHLTYVNFKNANLRNSNFSGKVLTG
                     VNFTGSNLSNAYLEYIDFTTVILY"
BASE COUNT          305 a          188 c          203 g          381 t
ORIGIN      
        1 catatagaat cactgttgtg aaatctatat attcaagata agcgttgcta aggttacttc
       61 cagtaaaatt aacgccagtg agaacttttc cgctgaagtt actgtttcgt agatttgcgt
      121 ttttgaaatt tacataagtc aagtggcgaa ctttacgaaa atcattattt tctaatgagg
      181 catttttaaa ttttgcatta cgcagatcac aatctgtaaa atccacgcct tcacagatcg
      241 taccggagaa cttggcatgt ctaagaatgc tgccattaaa attgacattc cttagaacca
      301 ttttagacaa gtctatgccg gttaaatcca tttctgctaa attcaaccct tcgatacacc
      361 gttgacccgt tgcatcttca ggaatagcag tgataatagt attgatgaca acatcaccta
      421 tcttgaaggt cattgtaatt cctgcatcag agatagagag ttttcgcgta agacgttcac
      481 tcttatctcc agtgatactc ttctgagcaa cgtaaaagcg ctcgcatgtg tcacaaaatc
      541 ttttccagct ctcatttggg gtcagattat tgtccgggag gaaattagca atgctataaa
      601 gatcgttcag gacgctactt cctatttcag gagaggcgca ttcaataatt tcagaggtgg
      661 atattgtatt tttaatttta tctatattat ccgtgagatt tcgtgatcct gctatatttg
      721 gctgctggtg gttttctttg tagtattgca gcaaattttc ccattgtaag tttgtaaacg
      781 agaaagtttc agtcttgtta ttgttatccc gtatggttaa ttgattttct actgggtcaa
      841 aagaaatcat gaagttattc aggtgttgat attgatgagt ggcatctaat ttcacttcaa
      901 atgaatttga acttttgcag gcatgtttta aaaacttttc acagggaagg tgttgatctt
      961 tatctacgtg ttgtgggtca ttgtgcaaaa cagagaaaat actctctttc aaattgttct
     1021 tgtcaatata gattgatagt tgcgcttttt gttccattat tttaataaac tgctgca
//
LOCUS       DAZGBC010000484         1073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGBC010000484 DAZGBC010000000
VERSION     DAZGBC010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.488"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1073
                     /locus_tag="ACHK1J_005446"
     CDS             <1..>1073
                     /locus_tag="ACHK1J_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS1414 family transposase"
                     /protein_id="HFO7914546.1"
                     /translation="RLLKKLSVEAALNAEMTHHLGYEKNQSRPGANSRNGYSTKTVIT
                     GDGPLELRTPRDRDGTFEPQLVKKNQTRITGMDNQILSLYAKGMTTREIATAFKELYD
                     ADVSPALISKVTDAVMEQVVEWQNRPLDAVYPIVYLDCIVLKVRQDSRVINKSVFLAL
                     GINIEGQKELLGMWLAENEGAKFWLNVLTELKNRGLNDILIACVDGLKGFPDAINTVY
                     PKARIQLCIVHMVRNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQQALEAFAAAWDSR
                     YPQISRSWQANWPNLATFFAYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSV
                     KKVVWLAIQSASQKWTMSLKDWR"
BASE COUNT          289 a          274 c          285 g          225 t
ORIGIN      
        1 atcggctgct gaaaaagctc agcgttgaag ccgctctcaa tgcagagatg acacaccatc
       61 ttgggtatga gaaaaatcag tccaggccag gagctaactc ccgcaacggt tattccacaa
      121 agaccgttat cacaggcgac ggtccactgg aactgcgtac tccgcgcgat cgtgacggta
      181 ccttcgaacc acaactggta aagaaaaatc agacccgtat taccgggatg gataaccaga
      241 tcctctcgtt gtatgccaaa gggatgacca cccgtgagat agctactgcg ttcaaagaac
      301 tgtatgacgc agatgtttca ccggcactga tatcaaaagt caccgatgcc gtgatggagc
      361 aggttgtaga atggcaaaac agaccactgg atgctgttta ccccattgtt tatcttgact
      421 gtatcgtcct gaaagttcgg caggacagtc gcgtcatcaa caaatcggtg ttcctggcac
      481 tgggcatcaa tatcgaaggt cagaaagaac tgctgggtat gtggctggcc gaaaatgaag
      541 gggcgaagtt ctggctcaat gtgctgactg aactgaaaaa ccgcggtctg aacgatatcc
      601 tcatcgcctg tgtggatggc ctgaaaggct tcccggatgc catcaacaca gtatatccga
      661 aggcccgcat ccagttatgc atcgtgcata tggtgcgcaa cagcctgcgc ttcgtgtcat
      721 ggaaggacta caaagccgtc actcgcgacc tgaaagcgat ttatcaggct cccacggaag
      781 aggcaggtca gcaggcactg gaagcgttcg ctgcggcctg ggacagtcgc tatcctcaga
      841 taagccgaag ctggcaggct aactggccga atcttgccac gttcttcgct tatccaacgg
      901 acatccgcaa agtgatctat acgacgaatg ccatcgagtc gctaaacagc gtgatccgcc
      961 atgcgatcaa aaagcgtaaa gtgttcccga cagacgactc ggtgaaaaaa gtggtgtggc
     1021 tggcaatcca gtctgcgtcc cagaaatgga cgatgtcgtt gaaggactgg cga
//
LOCUS       DAZGBC010000485         1070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGBC010000485 DAZGBC010000000
VERSION     DAZGBC010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.489"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1070)
                     /locus_tag="ACHK1J_005447"
     CDS             complement(<1..>1070)
                     /locus_tag="ACHK1J_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7914547.1"
                     /translation="AEQWQYDGHGWLTDISHLSEGHQVAVHYGYDDKGRLAGERQTVH
                     NPETGELLWQHETEHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLLEFT
                     RDRLHRETVRSFGNNAYELTSTYTPTGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPR
                     QTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIT
                     EDAHYLYRYDEYGRLTEKTDRIRAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLV
                     ESRYLCDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTRIQT
                     VYEPGSFTPLIRVETENGERKK"
BASE COUNT          145 a          364 c          318 g          243 t
ORIGIN      
        1 cttttttccg ttcgccgttc tctgtctcga cccggatgag cggcgtgaag cttcccggct
       61 cgtataccgt ctggatacgt gtggtgtcag tctgcaccgt cgtcagcctg tcgccgtccc
      121 atccgtacca tgtctcctcc ggtttacgcg acagcgacat ccaccccgtc aggtcacgct
      181 cccgccgcca gacccgtttt gccattcgcc gtcccagcgg gtcacagagg tagcggctct
      241 cgaccagtgg ctcaccatgc tgtatccgag tataaaacac caggcggtgc tgactgtcgt
      301 agtggtagtg gtgggtccgc tcgtcgtccg tccggataac ccctgcccgg atgcggtccg
      361 tcttctccgt cagcctgccg tactcatcgt agcggtacag gtagtgcgcg tcttcggtga
      421 tgcggttatc cggccacacc gtgagcgtgc tgtcaggatg cagctccggg tccggcaggc
      481 ggttgcccgc cgggtcggtg gcatacggga tgcggatgtc caggtccggt gcgagggtgc
      541 gcacgctctc cagcctgccc gtggcactgt agccgtattc ccgcgtctgt cgcgggccgc
      601 tgatgcgcac caggtcgccg ttgtcattcc acccgtagtc acggtcatac accaggctgt
      661 tcaggtgctg gctctgtaac tggcctgtgg gggtgtaagt gctggtcagt tcatatgcat
      721 tattgccgaa gctgcgcacc gtctcccggt gcaggcggtc gcgcgtgaac tccagcagcg
      781 gcgtcccgcc cagcttcatg cccgccaggt aaccgctgcc atacgtcagc cactccaccg
      841 gcggcaggct gtccggcgtg acgcggtttg ccagcccctg ttcgttgtat gcgtgctctg
      901 tctcatgctg ccacagcagt tcccccgtct ccgggttatg caccgtctgg cgctccccgg
      961 ccaggcggcc cttatcatca taaccgtaat gcaccgccac ctgatggcct tcgctcaggt
     1021 ggctgatgtc tgtcagccag ccgtggccgt catactgcca ctgctctgcc
//
LOCUS       DAZGBC010000486         1065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGBC010000486 DAZGBC010000000
VERSION     DAZGBC010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.490"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..499)
                     /locus_tag="ACHK1J_005448"
     CDS             complement(<1..499)
                     /locus_tag="ACHK1J_005448"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7914548.1"
                     /translation="MTLLEKSLVVIFSLLLIATVINGLLVWLRPGKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLP"
     gene            complement(496..>1065)
                     /locus_tag="ACHK1J_005449"
     CDS             complement(496..>1065)
                     /locus_tag="ACHK1J_005449"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7914549.1"
                     /translation="LLRPLMFWLNRRGVTANHITLCAMVLSLITGVVLIIHPQPGLFV
                     LLPVVLFVRMALNALDGMLARECNQRSRLRAILNETGDVISDISLYLPFMLLPQSSIP
                     LVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGLSLSIWPQIVAWN
                     NVIWSVATLLLLWTIFNRCRSALREEGVK"
BASE COUNT          325 a          298 c          256 g          186 t
ORIGIN      
        1 ccggtaacac cagtaaaaac gcgacatggc tgaaggcaaa tacggtggtc atcagactcc
       61 agtgcaactg ggaaacggta cgtaaaaagc cctgtgtatc cccagcaatc accattcgcg
      121 ccggtaaaaa aaggaacacg tagaccggga taaacaccac aaacatgccg taccagccga
      181 tacctatcag ccagtagtta aggggaattg caataaacat ccataacagc ggcatgcggt
      241 cggactggcg ggaaggcacc agcgtcaaaa actcttttag cgccataaag ctgaccagcg
      301 cgaagaacgt cagtgccagc cagctaggac ttaatagtgc gaaggaaaac aggacgataa
      361 tcacccacca ggtgcgaata cgtaatgtca gctcgcgcca gtctttacct ggacgcagcc
      421 acaccagcaa cccgttgatt acggtggcaa tcaacagcag ggaaaaaata acgaccagtg
      481 atttttcaag tagcgtcatt tcacaccctc ttcacgcaat gcgctgcgac agcgattaaa
      541 aatcgtccat aacaacaaca gtgttgcgac actccatatg acattattcc aggcgacaat
      601 ctgcggccag atcgacagtg acagccccca tgcaccaaac accagcgctc tgtcgctttt
      661 gcccagcgga ccggcatagc tgcgcacgcc gttaatcgtt tgcgccagaa cgccgcaaaa
      721 ctcggtcatc agcgcacaaa acaccacgat cagcaccagc ggtatactgc tctgcgggag
      781 tagcataaag ggcaaataaa gcgagatgtc ggaaattaca tcacccgtct cattgaggat
      841 ggccctcaga cgcgatcttt gattacattc acgcgccagc ataccatcga gcgcattgag
      901 cgccattcgt acaaacaaca ccacgggcag aaggacgaat aatccgggct gaggatgaat
      961 aatgagcacc acgccggtga taagggaaag caccatcgcg cacagtgtaa tgtgattagc
     1021 ggtgacgccg cgcctgttca gccagaacat cagcggtctt aacag
//
LOCUS       DAZGBC010000487         1065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGBC010000487 DAZGBC010000000
VERSION     DAZGBC010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.491"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..499)
                     /locus_tag="ACHK1J_005450"
     CDS             complement(<1..499)
                     /locus_tag="ACHK1J_005450"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7914550.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPGKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLP"
     gene            complement(496..>1065)
                     /locus_tag="ACHK1J_005451"
     CDS             complement(496..>1065)
                     /locus_tag="ACHK1J_005451"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7914551.1"
                     /translation="LLRPLMFWLNRRGVTANHITLCAMVLSLITGVVLIIHPQPGLFV
                     LLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLYLPFMLLPQSSIP
                     LVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGLSLSIWPQIIAWN
                     NVIWGVATLLLLWTIFNRCRSALREEGVK"
BASE COUNT          323 a          298 c          257 g          187 t
ORIGIN      
        1 ccggtaacac cagtaaaaac gcgacatggc tgaaggcaaa tacggtggtc atcagactcc
       61 agtgcaactg ggaaacggta cgtaaaaagc cctgtgtatc cccagcaatc accattcgcg
      121 ccggtaaaaa aaggaacacg tagaccggga taaacaccac aaacatgccg taccagccga
      181 tacctatcag ccagtagtta aggggaattg caataaacat ccataacagc ggcatgcggt
      241 cggattggcg ggaaggcacc agcgtcaaaa actcttttag cgccataaag ctgaccagcg
      301 cgaagaacgt cagtgccagc cagctaggac ttaatagtgc gaaggaaaac aggacgataa
      361 tcacccacca ggtgcgaata cgtaatgtca gctcgcgcca gtctttacct ggacgcagcc
      421 acaccagcaa cccgttgatt acggtggcaa tcaacagcag ggaaaaaata acgatcagtg
      481 atttttcaag tagcgtcatt tcacaccctc ttcacgcaat gcgctgcgac agcgattaaa
      541 aatcgtccat aacaacaaca gtgttgcgac accccatatg acattattcc aggcgataat
      601 ctgcggccag atcgacagtg acagccccca tgcaccaaac accagcgctc tgtcgctttt
      661 gcccagcgga ccggcatagc tgcgcacgcc gttaatcgtt tgcgccagca cgccgcaaaa
      721 ctcggtcatc agcgcacaaa acaccacgat cagcaccagc ggtatactgc tctgcgggag
      781 tagcataaag ggcagataaa gcgagatgtc ggaaattaca tcacccgtct cattgaggat
      841 ggcccccaga cgcgatcttt gattacattc acgcgccagc ataccatcga gcgcattgag
      901 cgccattcgt acaaacaaca ccacgggcag aaggacgaat aatccgggct gaggatgaat
      961 aatgagcacc acgccggtga taagggaaag caccatcgcg cacagtgtaa tgtgattagc
     1021 ggtgacgccg cgcctgttca gccagaacat cagcggtctt aacag
//
LOCUS       DAZGBC010000488         1064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGBC010000488 DAZGBC010000000
VERSION     DAZGBC010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.492"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1064
                     /locus_tag="ACHK1J_005452"
     CDS             <1..>1064
                     /locus_tag="ACHK1J_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HFO7914552.1"
                     /translation="TLTLDREVILPEAGTSTVNLINGSGKPVRVDITAHPAPDRIQVS
                     VLPDGMETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARF
                     EPMSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGSGENS
                     RLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELT
                     PGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGRQWSVSGPHIKP
                     GKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQ
                     LDDEMAEMKTTITETRNEIT"
BASE COUNT          235 a          293 c          341 g          195 t
ORIGIN      
        1 accctgacgc tggaccgtga ggtgatactg ccggaagcag ggacatcgac ggtgaacctg
       61 attaacggca gcggtaagcc ggtgcgcgtg gacatcactg cacaccccgc ccctgaccgg
      121 atacaggtca gcgtcctgcc ggatggcatg gagacatacg gtgtgtgggg actctccctg
      181 ccgtcactgc gtcgtcgcct gttccgctgt gtttccatcc gggaaaacac ggacggtacc
      241 tttgccatca cggcggtgca gcatgtgccg gaaaaagaag ccattgtgga taacggggcc
      301 cgctttgagc cgatgtccgg ctcactgaac agcgtcatcc cgccggcggt gcagcacctt
      361 acggtggagg tgagtgcctc agacggccag tatctggcac tggcgaaatg ggacacgccg
      421 cgggtggtga agggcgtgcg cttcagtctg cgcctgacca gtggcagtgg tgaaaacagc
      481 cgcctggtga ccagcgccat cacggcggac acggagcacc gtttcagtgg cctgccgccc
      541 ggggaataca ccctgacggt cagggcgata aacagctacg gccagcaggg cgaacctgcc
      601 accaccacgt tccggattaa cgcaccggca gcaccggcca gcattgaact gacgccgggg
      661 tattatcaga taacggcggt cccgcatctt acggtgtatg acccgacggt acaatttgag
      721 ttctggtttt cggaggcaaa aatcgcagac acatctcagg tggaaacctc tgcccgttat
      781 ctggggacag gccgccagtg gagtgtctcc ggcccgcaca ttaagccggg gaaggatttc
      841 tggttttatg tgcgcagcgt caacctggtg gggaaatctg cgtttgtgga agccagtggc
      901 cgggcgagca atgatgcgga agggtatctg gactttttca gaggagaaat cgggaagaca
      961 catctggcac aggggatgtg ggagctgatt gataacagcc agcttgatga tgagatggcg
     1021 gagatgaaga ccaccatcac cgaaacccgc aatgaaatca cgca
//
LOCUS       DAZGBC010000489         1060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGBC010000489 DAZGBC010000000
VERSION     DAZGBC010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.493"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..131)
                     /locus_tag="ACHK1J_005453"
     CDS             complement(<1..131)
                     /locus_tag="ACHK1J_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFO7914553.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQK"
     gene            177..1034
                     /locus_tag="ACHK1J_005454"
     CDS             177..1034
                     /locus_tag="ACHK1J_005454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914554.1"
                     /translation="MRITDNKKTNQAEYTFNADYFLIAVDTGNTAHFLTSVATGSTVR
                     FLTSAATGSTVHFLTSAATGSTVRFLTSAATGSTVRFLTSAATGSTAHFLISAATGST
                     VHFLTSSATGSTVRFLTSAATGSTVHFLTSTATGSTAHFLIPAATGSAAHFLISAATG
                     STAHFLISAATGSTAHFLISAATGNTAHFLITVTTGSTVRFLTSSATGSAAHFLISAA
                     TGSTAHFLISAATGNTAHFLITVTTGSTAHFLTSVATGNTAHFLITVATGNMVNDDYY
                     LNVQRQKQH"
BASE COUNT          219 a          312 c          185 g          344 t
ORIGIN      
        1 tccttctgat tattcctgcg tccctcccag agacggatcg catatcgggt atgaagggca
       61 atagtcagcg atgactgcag agcaccaact tgttctgatt ttctgacttc tccggttttc
      121 tcctggggca ttgtgtttac tccaaatgat ttcatgacca gaagatagtc cgcaggttgc
      181 gtattaccga caacaaaaaa acaaatcagg cagaatatac atttaacgcc gattattttt
      241 taatcgctgt tgacactggc aacaccgctc actttttaac ctctgttgcc actggcagca
      301 ctgttcgctt tttaacctct gctgccactg gcagcactgt tcacttttta acctctgctg
      361 ccactggcag cactgttcgc tttttaacct ctgctgccac tggcagcact gttcgctttt
      421 taacctctgc tgccactggc agcactgctc attttttaat ctctgctgcc actggcagca
      481 ctgttcactt tttaacctct tctgccactg gcagcactgt tcgcttttta acctctgctg
      541 ccactggcag cactgttcac tttttgacct ctactgccac tggcagcact gctcattttt
      601 taatccctgc tgccactggc agcgctgctc attttttaat ctctgctgcc actggcagca
      661 ctgctcattt tttaatctct gctgccactg gcagtactgc tcatttttta atctctgctg
      721 ccactggcaa caccgctcat tttttaatca ccgttaccac tggcagcact gttcgctttt
      781 taacctcttc tgccactggc agcgctgctc attttttaat ctctgctgcc actggcagca
      841 ctgctcattt tttaatctct gctgccactg gcaacaccgc tcatttttta atcaccgtta
      901 ccactggcag cactgctcat tttttaacct ctgttgccac tggcaacacc gctcattttt
      961 taatcactgt tgccactggc aacatggtta atgatgacta ctaccttaac gttcagagac
     1021 aaaaacagca ttaatgtcgt tatccccatc acgagcaact
//
LOCUS       DAZGBC010000490         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGBC010000490 DAZGBC010000000
VERSION     DAZGBC010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.494"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..614
                     /locus_tag="ACHK1J_005455"
     CDS             81..614
                     /locus_tag="ACHK1J_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7914555.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            664..>1059
                     /locus_tag="ACHK1J_005456"
     CDS             664..>1059
                     /locus_tag="ACHK1J_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7914556.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRI"
BASE COUNT          360 a          206 c          197 g          296 t
ORIGIN      
        1 aatctaaata ataacaacct gatcacataa tcgggtttga aaacgagtga ccttccatac
       61 cagttaatta aggatgattt atgaaaaagt atattattcc cttcgttgcg acaacgttga
      121 tgtttagcgc aacaaccttc gccaaggatg tcgatcaagg aacactaacc attagaggag
      181 aagttgttga tacaacgtgt tactttgtta atggtgattc gacaacagat atcgttgtcg
      241 aagatgttgt aaccagtgca atgaatcaac ttatcaataa tgaaatctat actttaaaaa
      301 ccggttcaac ctctcatgac ttagaaattc agtgtgacgg aaatacagcc ccaagtatcg
      361 aagtactagc atcagagttt gatgctaata actttacgct gaataaagga actgctgaga
      421 acattggctt tgccattttc ctcgatgatg ggactttgac cagaatgaga ccagggttaa
      481 aaacaccgct gaaagataat ggctctggtc aatattttct taatttgaat gcacaatatg
      541 cccgcacaag tggcaatcca gttacaaaag gctccgtaga gtcaaccgta acgattaaaa
      601 tcagtgcaga ttaacgtttt ttgccggtag ctctgctacc ggtattattt acggagtgat
      661 tacgtgagaa taataacagc attactgatg tcatttttta ttttacctgt tcatgccggt
      721 gtggtcatct atggtacgcg cattatttat cctgaaaaaa acagagaagt tctcgtacag
      781 ttaatgaatc aaagtaaaaa tgcctcttta attcaggcat ggatcgatga tgggaacacc
      841 actatcgcgc cagaaaaaat tcaggtccca tttattctca caccgccagt aagtcgtgta
      901 gcaggtggtt ctggccaaca acttaaaata agaaaaatgc caaacaactt gcctcacaat
      961 aaagaaagtt tattttatct aaatgttctg gatatccctc caaacaaccc acaaaatgca
     1021 ggaaaaaaca aaattaaact tgcattgcaa aaccgtata
//
LOCUS       DAZGBC010000491         1053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGBC010000491 DAZGBC010000000
VERSION     DAZGBC010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.495"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..279)
                     /locus_tag="ACHK1J_005457"
     CDS             complement(<1..279)
                     /locus_tag="ACHK1J_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HFO7914557.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNES"
     gene            436..1017
                     /locus_tag="ACHK1J_005458"
     CDS             436..1017
                     /locus_tag="ACHK1J_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7914558.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
BASE COUNT          344 a          188 c          218 g          303 t
ORIGIN      
        1 actttcgttt ttataatatg tctggataat atatttcaca tcccttttta cgtcttttaa
       61 tgtaaattca ggaatcacag gtttatatct gaaataccct gttattaata tcagatacac
      121 cctgctttct atatttatta ttttatcaat ttctctttta aggctgatat ccagcgaaaa
      181 aaactcatcc ctttcaacag gattaaaaga ggggacttga tataattcat ttatttcact
      241 ttttgttaat atactaattc ttttcttgta cttctgcata catttaccaa aatgctaaat
      301 atttgttaaa tatggctagt atcaaatatt aagatatctg atacctgtct aaaacagcat
      361 aaaagcaggt ttcaaaaatc gtattttgta cattatgata ccatgcagtg tcaaaaacga
      421 taagtgagac taaaaatggc gttattgggg tatgcacggg tatcaaccag tcatcagaag
      481 ctaacacatc aaatctcgga gctaaaaaat gccggggtac gggaagatcg tatttttacg
      541 gatatgatga ctggcgcaac tgatgaacgg gaaggactac agcggttgtt ggcccgtgca
      601 gagaaagacg acgtaatcat ctgtacaaaa atggaccgac tggggcgtaa cactgctgac
      661 atgattcgtg tggttgatag ctgttacaaa aaggggatcg ccatccggtt cctggagaac
      721 ggtctcagta cagaagggac aatgggcaaa atggttatcc aaatccttgc tgctgtcgct
      781 gaagcggaac gtgagcgtat tctggagcgg acaaacgacg gaagaatagc ggcacttgcc
      841 gcaggcgtaa aatttggcag gaagaagcat ccacgaacgc ctacagcgtt ggagcttata
      901 tcacagggag aatctctggg cagtgtgaca gaaaaaacag gtatctctcg atcgacgtac
      961 ttcaggttaa aaagaacaat aaaaaatgat gctaaaattg caacgtttta taaataaatt
     1021 caattaatta cctaagcaag gtgagacttt tag
//
LOCUS       DAZGBC010000492         1053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGBC010000492 DAZGBC010000000
VERSION     DAZGBC010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.496"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..162
                     /locus_tag="ACHK1J_005459"
     CDS             <1..162
                     /locus_tag="ACHK1J_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO7914559.1"
                     /translation="PQPDRDRRRQIRTRWSLTDDVIPRRHDNDEGSETGETTETTETR
                     HPADKEKAQ"
     gene            159..>1053
                     /locus_tag="ACHK1J_005460"
     CDS             159..>1053
                     /locus_tag="ACHK1J_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO7914560.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELSALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLNAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSAAPAITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNQTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTG"
BASE COUNT          282 a          318 c          254 g          199 t
ORIGIN      
        1 cctcagccag accgcgatcg cagacgtcag ataaggaccc gctggtcact cacggatgac
       61 gtgataccac gccgccatga taacgatgaa ggctcagaga ctggtgaaac caccgaaacc
      121 actgaaaccc ggcatcccgc cgacaaggaa aaagcacaat gaccatccgc tacactgcaa
      181 cactgaacaa taaacacctg tccctgaccg ccctgtaccc tgacggtggt caggaggtta
      241 ttgccatcct gccggttaca gaactgagcg cactgattaa ccgtaataac aggctgatgc
      301 acctgtcctt cagcacccct tttcgtgaaa taaccgaaac actgcttgtt gataccgttg
      361 atactgaacg tcccggacat cacatcggca gaatgctctc cgcgattgac agctgtctga
      421 acgcggcata tgcagaactt cctgcccgga aaaccagctt catgagaggg ctgatatacg
      481 gtgcaggatt agtgctggca cttctgacag cggtaacagc cggatatatt ttactgaaac
      541 cggacagcga ttcccctctc agggtacctg tcacagaatc cgtaacagaa actgcaccat
      601 tacacatcga caaggaaatt gtccggtcag tagccccgtc agagacgaaa aatgaaatcg
      661 ttcacccggc accacaggaa atgcaggaga catccgctgc cccggcgata acagcagaca
      721 aaaatactct ggtcgctgaa gcccgccgta atctggccac tgtacttaaa cgcaacgcgg
      781 accggggtat gttcaccatt aatctctcca ctggtcatga acgcacactc tacgccttcc
      841 ttgaccccgc atgcatcaac tgccgtcttc tggagcccgc gcttaagcaa ctggcccggg
      901 aattcagcgt ggtgatttat ccggtctccg taatcggcgg agaggccagc acaaaccaga
      961 ctgcgccact gctgtgtgaa ccggacgcac aaaaacgggc gaccggatgg catcagctgt
     1021 attccgcaga cggtggtatg gcacccaccg gcg
//
LOCUS       DAZGBC010000493         1049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGBC010000493 DAZGBC010000000
VERSION     DAZGBC010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.497"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..404
                     /locus_tag="ACHK1J_005461"
     CDS             <1..404
                     /locus_tag="ACHK1J_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO7914561.1"
                     /translation="VALADICARLNGRAPKSVCWMARRFGLKRLGYWSAEEDEILRQY
                     YPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRLWTAEEWNVLSGNMASGLSA
                     LMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
     gene            596..1049
                     /locus_tag="ACHK1J_005462"
                     /pseudo
     CDS             596..1049
                     /locus_tag="ACHK1J_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          333 a          183 c          261 g          272 t
ORIGIN      
        1 gcgttgcgct tgctgatatt tgtgcccgtc tgaacggacg agcgccgaaa tccgtgtgct
       61 ggatggcccg caggtttggt ctgaaacgcc tgggttactg gagtgcagaa gaagatgaaa
      121 tcctgcggca atattatcct tcagaaggtg tgaaagtgag ccgacgcctt tccggacgta
      181 cagaaaatgc agtgaaactg agagcgcggg agctgggtgt aaagttcacg ggaaacgggc
      241 gattacgtct ctggacggca gaggagtgga atgtgttgtc cggaaatatg gcatcggggc
      301 tatcggcact gatgaagtta ctgccggggc gttcccgtag ttcagttcag ggggctatga
      361 ggcgtttaca gaaaatgagt gcgcagaaat ctgtcaggca gtagccaaaa tattcctgca
      421 tcaggagaac ggcagtggca ataataagca gcaaactgat acataatgca gcttctgtaa
      481 acggtaatga tcaagtaggg atatttttat gttgactggt tgtgaaaaaa tgatcaaaat
      541 ttaaattaac ctttgcatga gattagaatc tattaatttt atcaaaggag attgtgtgac
      601 aatagtaaga gattctgtta gtggtgaaat tgttcatagt catgatcttc gggaaatgga
      661 tgttctttat atacaaaaaa ccacttttga atgtccgtat gacaaatgta ggatacctgc
      721 tactccatgt agcttcaaag aaataaattt aaatcaatca tactttcgat ataaagagcc
      781 tcgtaggccg ggatgtaaga ttcatgatcc taagagtaaa ataagtataa ctcaaagaat
      841 aataaaaagc ataattcacc accggcactg gctatatatc ttctgaaact tgatgtacct
      901 gaaggaaatt acgactcttc tggcagaaga attaataaac aggcgcaaga tgatgaaaaa
      961 gaattggggg gcaaagtaca ccctgtgtca tcttcaagca tcaaaccggt agtagactac
     1021 tacattaatg gagataatct ctatgaacc
//
LOCUS       DAZGBC010000494         1048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGBC010000494 DAZGBC010000000
VERSION     DAZGBC010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.498"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(226..576)
                     /locus_tag="ACHK1J_005463"
     CDS             complement(226..576)
                     /locus_tag="ACHK1J_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914562.1"
                     /translation="MKIKLGSGSVSVNGVVYEGKGKIISILNNEVIIDGVVHGKAVAL
                     PEITVIVNGHVDSLHTELGNIIINGNAGMVKTASGDVSCENVSGNVESMSGNVNCKNV
                     YGGVKTLSGNIYKE"
     gene            complement(609..884)
                     /locus_tag="ACHK1J_005464"
     CDS             complement(609..884)
                     /locus_tag="ACHK1J_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914563.1"
                     /translation="MSQYRLTGKSASRSYELLVGWDTPLSRFFLVIEPELDEPVYSNI
                     YEKDPSSLTLEFFQSVLERYGIENVSLLPGHESGLYEKLHDDRRNNN"
BASE COUNT          357 a          231 c          151 g          309 t
ORIGIN      
        1 tgattatcga atcaagtgat tgtataccaa aaaatcaggg gtacgcagat acggcagata
       61 acgaaaaaca gtccctggat tgatgatttt gtgaactatc tcattaagaa aaaataaata
      121 cctgataaaa tgaaaatggt ttctgcatat aacctggaaa tgtagcaacc caatacctct
      181 tctgtactac ccccgtacaa tatattgtac ggggttttaa tgaactcatt ctttatatat
      241 gtttccagat aatgttttaa ctccaccata aacattttta caattaacat taccactcat
      301 agactcaaca tttcctgaaa cattttcaca agaaacatcc cccgatgcag ttttaaccat
      361 accagcatta ccattaatga ttatatttcc caactcggta tgtaatgaat caacatgacc
      421 attaacaata acagtaattt caggaagagc tacggctttc ccatgaacga caccatcaat
      481 aatgacttca ttatttaata tggaaataat tttccccttt ccttcataca caacaccatt
      541 tacagatacc gaacctgacc ctagttttat tttcatcgca atattcctgc atattccatt
      601 aaaaaccgct agttattatt tcttctatca tcatgcaatt tctcatacaa acctgactca
      661 tgaccaggca gcagagatac attctctatc ccgtaacgtt caagaactga ctgaaaaaac
      721 tcaagcgtca aagatgatgg atctttctca taaatattcg aatataccgg ctcgtcaagt
      781 tctggctcaa taacaagaaa aaacctggaa agcggggtat cccagccaac cagaagttca
      841 taactacgcg atgctgattt tcctgttaaa cgatactggc tcataactcc ctcctgaata
      901 attcacgctc aaaaacaatc atataatcat atgattacaa aatcaaggga ttatatcctt
      961 ttgttattct tataggggtt ccgtgggaat ttctgcgata acacccgcta agcggagata
     1021 cgtctggata acgcaaatga ttgcggta
//
LOCUS       DAZGBC010000495         1047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGBC010000495 DAZGBC010000000
VERSION     DAZGBC010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.499"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..>1047
                     /locus_tag="ACHK1J_005465"
     CDS             76..>1047
                     /locus_tag="ACHK1J_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7914564.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDVSKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFP"
BASE COUNT          332 a          192 c          241 g          282 t
ORIGIN      
        1 aatgcaaatt ttcgatttat tttccatcat taatccagat aaacaacaaa ctaatagtat
       61 gcaaggagac attatttgtt tcgccaggat gctttagaaa acagaaaaat gaagtggcag
      121 ggacgggcaa tattacttcc cggaatacca ctgtggttaa tcatgctggg aagcattgtg
      181 tttattacgg catttctgat gttcatcatt gttggtacct atagccgccg tgttaatgtc
      241 agtggtgagg tcacaacctg gccaagagct gtcaatatat attcaggtgt acagggattt
      301 gttgtcaggc agtttgttca tgaaggacag ttgataaaaa aaggggatcc tgtttatctg
      361 attgacgtca gtaaaagtac acgtagtggt attgtcactg ataatcatcg tcgggatata
      421 gaaaaccagc tggttcgtgt ggacaacatt atttcccgtc tggaagaaag taaaaaaata
      481 acgctggata ccctggaaaa acaacgtctg caatacacag atgcgtttcg tcgctcatca
      541 gatattatac agcgtgcaga ggaagggata aaaataatga aaaacaatat ggagaattac
      601 agaaactatc aggcaaaagg gctgattaat aaagatcagt taactaacca ggtggcatta
      661 tattatcagc aacaaaacaa tcttctcagc ctgagcggac agaacgaaca gaatgccctg
      721 cagataacca ctctggagag tcagattcag actcaggctg cagattttga taaccgtatc
      781 taccagatgg aactgcaacg gtacgagtta cagaaagaac tggttaacac tgatgtggag
      841 ggcgaaatca ttatccgggc gttgactgac gggaaagttg actccctgag tgtcactgtc
      901 gggcaaatgg tcaataccgg agacagcctt ctgcaggtta ttcctgagaa cattgaaaat
      961 tattatctta ttctctgggt cccgaatgat gctgttcctt atatttcggc tggtgacaaa
     1021 gtgaatattc gttatgaagc ctttccg
//
LOCUS       DAZGBC010000496         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGBC010000496 DAZGBC010000000
VERSION     DAZGBC010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.500"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..93)
                     /locus_tag="ACHK1J_005466"
     CDS             complement(<1..93)
                     /locus_tag="ACHK1J_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914565.1"
                     /translation="MPNWCSNRMHFSGEPAQIAEIKRLASGAVTP"
     gene            complement(179..490)
                     /locus_tag="ACHK1J_005467"
     CDS             complement(179..490)
                     /locus_tag="ACHK1J_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914566.1"
                     /translation="MNYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            774..1022
                     /locus_tag="ACHK1J_005468"
     CDS             774..1022
                     /locus_tag="ACHK1J_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7914567.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
BASE COUNT          263 a          295 c          288 g          200 t
ORIGIN      
        1 cggtgtgacg gcaccactgg ccagtcgttt aatctcagca atctgtgccg gttcaccaga
       61 aaaatgcata cggttactgc accagtttgg catgggaatt ctcctctcac ttctgcttca
      121 gcagagagca ccgggagggc gcccctgtag cgcccgttcc ccgctgcctg tgggctgttc
      181 accgcgtccg gtaacgagtc aggccgaccg tggaataccg cagccgtcgt ctttttgacg
      241 gcaaggctat gccgcgaaag cggcttgaag ggcgttcatc agaaaagggc gacgggctgg
      301 cgtacactgt caccgacgcc agccgggagc ggtaaaagcg cccgtcccgt gcccgccgga
      361 cgcgggacac tgaccacagg cagcgtttat gccgcgccgc gtcagccggc gcagtaaaaa
      421 gaggagcacg gcgacatcct ttaccggaaa agaaacgggc ggttgcccgc ccgtctccgt
      481 cataattcat ccaccgtaag tgaccgggtg aggaggccgg cgacagcgac acacttgcat
      541 cggatgcagc ccggttaacg tgccggcacg gcctgggtaa ccaggtattt tgtccacaga
      601 accgtgcaca aaatgctgtg gataagctgg acacagcagc ccacagcagg catatagcca
      661 cactctcaga gaattgagcg ttttttagtc acaataatga catttgactg tgtgtggatt
      721 cagttattag aatgctgtat atacatacag caaaagaaaa gggagatgag aacatgatcc
      781 gcattgaaat tcttttcgac cgccagagca caaaaaacct caaatcaggc acacttcagg
      841 cgctacagaa tgaaatcgaa caacgtctga aaccacacta cccggaaatc tggctgcgta
      901 tagaccaggg cagcgccccg tctgtatctg tcacaggagc ccgcaacgac aaggataaag
      961 aacgtatcct gtctctgctg gaagaaatct ggcaggacga cagctggctg cctgcagcat
     1021 gagtaccgtt tatcaccgtc cggctg
//
LOCUS       DAZGBC010000497         1043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGBC010000497 DAZGBC010000000
VERSION     DAZGBC010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.501"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1043)
                     /locus_tag="ACHK1J_005469"
     CDS             complement(<1..>1043)
                     /locus_tag="ACHK1J_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914568.1"
                     /translation="NSPEVRDWLSHERIEQLEESGIDIKKLANPVLLAYLRSLENENF
                     NNLCERSSKIVEHYFQAMLERERERQDLLMTPEKQSSILTMVALDMCENDYTSDNRDK
                     IISLIKEKCLFILEETRQLYTGKDRPTLDALASKLATHAFFDRSNQGENKIEFVNEFV
                     FGNYIANSIIANDSDDWLANDERFVEPAVQAYLPRDSHDRELLWTRLSGMREFLSSSD
                     HMKYEYLLTQEIDASIYSETTITGLSFDNALFFQHSEIKNSVFNECVFSNVVFNFNNF
                     DDVTFISCQFYDCDYNKENHPYNISFLNCSDNNEIINEIEFYEITEDSENLELQLERE
                     VLSKFMPLGSCSIT"
BASE COUNT          333 a          188 c          148 g          374 t
ORIGIN      
        1 gttatagaac atgaacctaa aggcatgaat ttagaaagga cttctctttc taactgtaat
       61 tctaaatttt cactgtcttc tgttatctca taaaattcaa tctcattaat aatttcatta
      121 ttatcagagc agttaagaaa acttatatta taaggatggt tttccttatt ataatcacaa
      181 tcatagaatt gacaggatat gaatgtaaca tcatcaaaat tgttaaagtt gaataccaca
      241 tttgaaaaaa cacactcatt gaatacagag ttttttattt cagaatgctg gaaaaataaa
      301 gcattatcga aagacaaccc agtaattgtt gtctcactgt aaattgaggc atcaatttct
      361 tgcgttaata aatactcata tttcatatgg tcactcgaac tcagaaattc acgcattcca
      421 cttaatcgtg tccatagtaa ttcacgatca tgactatctc tcggtaaata tgcttgaaca
      481 gctggttcaa caaagcgctc atcattagct agccaatcat cagagtcatt ggcaattata
      541 ctattagcga tataattgcc aaatacgaat tcattgacaa attctatttt attctcacct
      601 tgattgcttc tgtcaaaaaa agcatgtgtt gctaatttac ttgctagagc atctaaagtt
      661 ggcctgtctt tacctgtata taactgtctg gtttcttcta atataaaaag gcatttttcc
      721 tttatgagag atattatttt atctctatta tcacttgtat agtcattttc acacatatct
      781 aaagcaacca tagtaagaat ggatgattgc ttttcaggag tcataagcaa gtcctgtctc
      841 tctctctctc tttccagcat tgcttgaaaa tagtgttcaa caatttttga acttctttca
      901 catagattat taaagttttc attctctaat gaacgcagat atgctaaaag aactgggttt
      961 gctaatttct ttatatctat gcctgattct tcgagttgtt caatacgttc atgacttagc
     1021 caatccctga cttcgggtga gtt
//
LOCUS       DAZGBC010000498         1028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGBC010000498 DAZGBC010000000
VERSION     DAZGBC010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.502"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..722
                     /locus_tag="ACHK1J_005470"
     CDS             <1..722
                     /locus_tag="ACHK1J_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7914569.1"
                     /translation="PDISDITPCLIYYHGGCFVSGGFETHDNQLRQLAYYSGCRVIAV
                     QYRLAPEFLFPVAHDDAERGACIIWQNAESLGIDRNRISLAGDSAGGHLALVTALRLK
                     SVAQWQPSQLILIYPMLDATACLESYAMFGDDYVITRDALVSGYDMYLHDTDREHPEA
                     SPLWRSDLQGLPATHIITAEFDPLRQEGEMLYQRMTEQGVMCTCQRYLGVIHGFFQLG
                     GISQAGRDAVQAVASRLSMFS"
     gene            955..>1028
                     /locus_tag="ACHK1J_005471"
     CDS             955..>1028
                     /locus_tag="ACHK1J_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein pndA"
                     /protein_id="HFO7914570.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMV"
BASE COUNT          250 a          210 c          272 g          296 t
ORIGIN      
        1 gaccagatat atcagatatc actccatgtc ttatttatta tcatggcggt tgcttcgtca
       61 gtggtgggtt tgagacccat gataaccagc taagacaact ggcttattat agtggttgtc
      121 gtgttatcgc agttcagtac cgtcttgctc ctgagttttt gtttcccgtg gcgcacgacg
      181 atgctgagag aggagcctgc atcatatggc agaatgcgga atctctggga attgaccgga
      241 acaggatttc tctggcaggt gacagtgctg gaggtcatct tgctcttgta acggcattac
      301 gccttaaatc agtagctcag tggcaacctt cacaacttat tcttatttat cccatgcttg
      361 atgcaacagc atgcctggaa agttatgcca tgtttggtga tgactatgtt attacgcgag
      421 atgcacttgt atcaggttat gatatgtact tacatgatac ggatcgtgaa catcctgaag
      481 cctcgcctct ctggcgttcg gatttacagg gactgcctgc tactcatatt atcacagctg
      541 aatttgatcc tttacggcag gaaggggaga tgttgtatca gcgaatgact gagcagggcg
      601 taatgtgtac gtgccagcga tatctggggg ttattcatgg tttctttcag cttggcggta
      661 taagtcaggc tggaagagat gcagtgcagg cagtggcatc ccgattgtcg atgttttcct
      721 gaatcatgtg caggtaatct actgtaagtc agaaagttga aaacgattgt ggctcactga
      781 aaaacaatta gaatagcggc gggcgcttga ggctgtatgc cgaaagcgtt ttgtggacgg
      841 catgcagcag aaagccccag gtgatctttt tatcaatcaa cccagggccc actgtaatgc
      901 ctcaacaaca ttatagtagc ccgatgacgg ccgaaaggca atggaggggc tgcgatgcca
      961 caacgaacgt ttttaacaat gttaattgtc gtctgtgtga cgatactgtg ttttgtctgg
     1021 atggtgag
//
LOCUS       DAZGBC010000499         1021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGBC010000499 DAZGBC010000000
VERSION     DAZGBC010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.503"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..436)
                     /locus_tag="ACHK1J_005472"
     CDS             complement(<1..436)
                     /locus_tag="ACHK1J_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914571.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVE"
     gene            complement(672..881)
                     /locus_tag="ACHK1J_005473"
     CDS             complement(672..881)
                     /locus_tag="ACHK1J_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914572.1"
                     /translation="MCVGCGYSGVRDGAGKNALVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
BASE COUNT          267 a          316 c          249 g          189 t
ORIGIN      
        1 gttccacccg ctcttcagca tcaaatgcca gtgcagcaag gcttgcccag cacatgcagt
       61 tcactgaatt ccccggaacg ctctgcagct cccggcgcac catgttgacc tcagcactga
      121 aaaaagcgtt ctcatcgcct tcaagggcag ccagatgcag cggaagccag acctgactga
      181 accattcgca catccgttcc cggttactga cggtcatcac tgtctccggg cgggaaccgt
      241 cctgaaaaac atgtccggtg cgtggagaac tgtatgaaac ataaccaccg tgcaccgcaa
      301 tgaccggacg acctgacgcc ggaagcccca cacgacaccc actggcaatc cgggtcatca
      361 gcaggttcag ttcagccagt aattcagggc gggttaagac cgccggaacc ggagaatatt
      421 tcactgagga agacatactt tcacctccat atctctcagc ccgccaggtg cgtcacttcc
      481 ccgcagagaa ccggaaacct ggctatatga aaaaagggaa aagggtaccg gacgaacagt
      541 tgtcgggaat aaaatcccgg taactgtctg tacgttttca gaccggtaag tttggcgacc
      601 ctggtcagaa ggtacggaca gcatctttgc gctcataact gtaccgaccg ggatatgcat
      661 tacaaagact gtcataccgc cccctccgtc tttcgctgtt tgcaggaaag accacatgaa
      721 aaaagctggt catgccgctg acgacgctgg cgttcctgca aaagaaagga gtcacaaccg
      781 gaacacagcc ccagaccact ctcaggattc accttcaccc agaccagagc attcttaccg
      841 gcaccatccc gtaccccgga ataaccacag cccacgcaca ccgtccactt cttcagtacg
      901 atgagtgcag tttcgagtga tgcgcggaga cgccgggagg caataaggag caacggctca
      961 tggtcataaa aaaccccgcc gttaccccct ccggattcaa gcagcgggag tcctgcaggc
     1021 t
//
LOCUS       DAZGBC010000500         1019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGBC010000500 DAZGBC010000000
VERSION     DAZGBC010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.504"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..171)
                     /locus_tag="ACHK1J_005474"
     CDS             complement(<1..171)
                     /locus_tag="ACHK1J_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO7914573.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAV"
     gene            complement(168..392)
                     /locus_tag="ACHK1J_005475"
     CDS             complement(168..392)
                     /locus_tag="ACHK1J_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7914574.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            458..813
                     /locus_tag="ACHK1J_005476"
                     /pseudo
     CDS             458..813
                     /locus_tag="ACHK1J_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          285 a          266 c          228 g          240 t
ORIGIN      
        1 cacggcagcc agatccataa tatctgtcac accttcataa tgatacatgt tcaaaacacg
       61 atgaatgatc gcgtcagcgc gaccgggttc tggcataccc gccacgccac catcacgcga
      121 aatcaagcgg tcatgaaact caatcatttc ctgtgaacta acgaatttca tcgcttagaa
      181 agctccgtat aggccgtccc atgactttgc atgatctcag caaactcagc atcaagagcc
      241 gctttacggt atcgttcaaa agcgtcttta tcaaccagta cagccgcgcc acctttacgg
      301 cgctgaatct caacaggctg attttcatca accgccagct cgagcaacga ggccaggtta
      361 tcgcgagcat ggctatagtt acatgtaaac atagtgcttc ctctttgagt tgtacaatta
      421 aattgtacaa cagttatctt caatatgaaa tcaccgttac cgctggcgca tcctgaccca
      481 acttaaccgg ggagaaagtc gccatgccgt tgccagagct atctgtcacg gtcagaaagg
      541 tgagataaga aagcgatata ccgatggtca agaagatcaa ctgggcgcac tggggctggt
      601 gactaacgcc gtcgtattat ggaacactat ttatatgcag gctgccctgg accatttcag
      661 ggcacagggg gaaatcgtga atgatgaaga tatcgcacgc ctctcaccac tttgccacgg
      721 acacatcaat atgctcggcc atattccttc acgctggcag aactggtgac gaaagggcaa
      781 aaagaggcat cggaggaaga aaacattgct taacgtgagt tttcgttcca ctgagcgcca
      841 gccccctaat tctctatatc caaactgacc ccaataggtg acgggttatg tgacgaaatc
      901 tgatgtagaa atcgttgttt cagtgacagt cactcaatcg gtcatttatg tgacacccca
      961 cgccgttact ggtcgcggaa aaatccaggt ttccggcttg gaaccccatc tcaagagat
//
LOCUS       DAZGBC010000501         1009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGBC010000501 DAZGBC010000000
VERSION     DAZGBC010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.505"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..344)
                     /locus_tag="ACHK1J_005477"
     CDS             complement(<1..344)
                     /locus_tag="ACHK1J_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7914575.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLCDGWYQVRIAGRDVSTSGLTVQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVL"
     gene            complement(341..>1009)
                     /locus_tag="ACHK1J_005478"
     CDS             complement(341..>1009)
                     /locus_tag="ACHK1J_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7914576.1"
                     /translation="STPEGERYFPCVNISGEPEDYFRMAPEDWLQAKMQGEIVALVHS
                     HPGGLPWLSEADRRLQVQSDLPWWLVCRGVIHKFRCVPHLTGRRFEHGATDCYTLFRD
                     AYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEATGFYRVALTEAQPGDVLLCCFGSSVP
                     NHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFTGIYNDLAAA
                     STFV"
BASE COUNT          233 a          333 c          264 g          179 t
ORIGIN      
        1 agcaccatac tggcaccgag agaaaacagg atgccggtca taccaccggc cccaatggct
       61 gccccccatg ctgcaagggt ggctccggca gtaaagaatg atccggcaat ggcggcagct
      121 cccaggacaa tctggaatac gccacctgac ttggccccgg cgactctggg aacaatatga
      181 atcacagcgc catcaggcag agtctcatgt aactgcaccg ttaacccgga cgtgctgacg
      241 tcccgcccgg caatccgtac ctgataccag ccgtcgcaca gtttctgacg aaacgccggg
      301 agctgtgtgg ccagtgcccg gatggcttca gcccccgttt tcacacgaag gtcgatgcgg
      361 cggccaaatc gttgtaaatc cccgtaaagg cagatgcgcg ccatgcccgg tgacgccaga
      421 gggagtgtgt gcgtcgctgc catttgtcgg tatacctctc tcgtttgctc agttgttcag
      481 gaatatggtg cagcagctcg ccgtcaccgc agtaaatagc ggcatgattc ggcaccgatg
      541 aaccaaagca gcacagcagc acatcgcccg gctgcgcctc tgtcagtgcg acacggtaaa
      601 aaccagtcgc ctccatattg tcaagataga gattctgacc gtgacgccac cagtcatccc
      661 cgcgatgaaa atccggcatc tcaatcccag ccagatgata agcatcccgg aacagcgtgt
      721 aacagtccgt cgccccgtgc tcaaagcgcc gcccggtgag atgcggcaca cagcggaact
      781 tatgaatcac tccccggcag accagccacc acggcaaatc actctgcacc tgcagccgcc
      841 ggtcggcctc actcagccag ggcagaccac cggggtgact gtggaccagc gccacaatct
      901 caccctgcat tttggcctgc agccagtcct ccggagccat ccggaaataa tcctccggct
      961 caccggaaat attcacgcag ggaaaatatc tttccccctc cggcgtgct
//
LOCUS       DAZGBC010000502         1008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGBC010000502 DAZGBC010000000
VERSION     DAZGBC010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.506"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1008
                     /locus_tag="ACHK1J_005479"
     CDS             <1..>1008
                     /locus_tag="ACHK1J_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7914577.1"
                     /translation="TNGTAYAMRTDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKL
                     DIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITAKDAGDKTTWNLTGYRMAENPAATQ
                     SASGLASVGYKSFLSEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHV
                     QVGVDKKHELDGLDLFTGFTVTHTDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDL
                     IGKYVHHDNEYTATFAGLGTRDYSTHSWYVGAEAGYRCHVTEDTWIEPQAELVYGAVS
                     GKQFVWKDQGMHLSMKDRDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANG
                     ET"
BASE COUNT          269 a          223 c          282 g          234 t
ORIGIN      
        1 gacgaatggc actgcgtatg ccatgcgtac ggacctgaaa aatgcggata agctggtagt
       61 aaaccaaaag ctgtcaggta aggataatat tctgctggtt gattttctga acaaacccac
      121 cggagaaaaa ctggatattg aactggtgag tgcaccgggg aacagcagta aggatgtttt
      181 taaaggaagt gaacaggcaa taggtttcag caatgtcaca cctgttatta cagctaaaga
      241 cgccggagat aaaacaacat ggaacctgac cgggtacagg atggcagaaa atcccgccgc
      301 aactcaaagt gcctcaggcc ttgcatctgt ggggtacaaa tcatttttga gtgaggtcaa
      361 caacctgaat aaacgtatgg gtgacctgcg tgacatcaat ggtgaagctg gcgcatgggc
      421 acgtatcatg agcggaaccg gctctgccgg tggtggtttc agtgacaacc acacacatgt
      481 tcaggtcggt gtcgacaaaa aacatgagct ggacggactg gatttgttta ccggcttcac
      541 tgtcacacac actgacagca gtgcctctgc tgatgctttc aaaggtaaaa cgaagtctgt
      601 gggggccgga ctctatgctt ccgccatgtt tgattccggt gcctatatcg acctgattgg
      661 taagtatgtt catcatgata atgagtacac cgcaaccttt gccggactcg gaacccgtga
      721 ttacagtacg cattcatggt atgtcggtgc tgaagcaggc taccgctgtc atgtcactga
      781 ggatacctgg attgagccac aggcagaact ggtttacggt gctgtatccg gtaaacagtt
      841 tgtatggaag gaccagggga tgcatctgtc catgaaggac agggactaca atccgctgat
      901 tggtcgtacc ggtgtggatg tgggtaaatc cttctcaggt aaggactgga aagtgacagc
      961 ccgtgccggt ctgggctacc agttcgacct gctggctaac ggcgagac
//
LOCUS       DAZGBC010000503         1004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGBC010000503 DAZGBC010000000
VERSION     DAZGBC010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.507"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(94..208)
                     /locus_tag="ACHK1J_005480"
                     /pseudo
     CDS             complement(94..208)
                     /locus_tag="ACHK1J_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            589..>1004
                     /locus_tag="ACHK1J_005481"
     CDS             589..>1004
                     /locus_tag="ACHK1J_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO7914578.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLE"
BASE COUNT          247 a          231 c          247 g          279 t
ORIGIN      
        1 ccagaagtaa tattccttac catcaaaatt catcatcttt gccaaagaaa aatgttcaga
       61 aaataatcca tggaaaattg tccggagcgc ttactatttt aatggattgt tagtctttgc
      121 atgagcaagc ggactgatac atttctcttt gttctcattc agaaaatctc atcagtcggc
      181 gttcagaccg gtgttgtgct ttatggatgc gttacgctcc tgatgacgtc atttgacgtt
      241 caacaacatc acggggccgc acgacatttc acgtcagtta gtgctatcgc tcaggaacaa
      301 attttcccga attgggatat gcccgcaaat tgctggtgat gaggggagaa tctggttgag
      361 ttcgggagaa ttgatttgga ggcagaacgc ttaaatcgtg gcgtcctgaa acgaaaaacg
      421 gacctccgtg gaggtccgtt tatatgaatt tggtgccgga ctcggaatcg aaccaaggac
      481 acggggattt tcaatccccg tgttgcgagt attcaggtag tgaagtgaat attctggctg
      541 gtttccgtag tgtaagtgat tggttgcata ctttaaagag tgtgctatat gaaaatttta
      601 ccggttatat ccactaaagg tggcgaagga aaatcaaccc agtctgctaa tcttgccggt
      661 tttctggcag atgccggcct ccgtactctg ctgatagacg gagaccatgc acagcccacg
      721 gcaagcaata tctttccgct gacgtatgaa gcgccggccg gtctttttga attactgatg
      781 cgcacggctg acctcagtca tcctgacaac atcatttccc gtacggctgt cagggggctt
      841 gacctcatcg tctccaacga cccccatgag caactgaaaa cggccatgct tcatgcgccg
      901 gatggtcgcc ttcgtctgcg taatgtgctt cagcatccgc tttttcagac ctatgacgtc
      961 attgtggtgg attcacaggg cgcacgttct gtcatgctgg aaat
//
LOCUS       DAZGBC010000504          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGBC010000504 DAZGBC010000000
VERSION     DAZGBC010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.508"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            512..>987
                     /locus_tag="ACHK1J_005482"
     CDS             512..>987
                     /locus_tag="ACHK1J_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7914579.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG"
BASE COUNT          229 a          256 c          276 g          226 t
ORIGIN      
        1 attgctggtt tcgtggtgtg cagcaatgta gttacagtgt aatcaatgtc acaattcagt
       61 cagttgaagg ttgtctgccc gactgagaat ttgttaaaaa aaatcctgca tggtgaatcc
      121 ccctgagcgg cggggcatat cagcgtcaca ggtgtttctg ttttacctct atcctttctg
      181 tgcgggttca ggtgctgata ctgaactcac cgggaggcac ccggcaccat gcatgaacgg
      241 tacatagcgc atacatcagc ccctctccgg aggggctttc ttgtgggcaa aaaaaagccc
      301 gcgccgggag acgcgggcgg caaggaataa acaacaaaac gtgaagtaat atttcagctg
      361 gcgaataata cccgacagta atcactctgt gcaactgcgc ggcctttttc gtattgcggg
      421 ctgtagtctc ctttctgcca ttgtcctgta acttccggac ttcagcccgc tccttatctg
      481 actcacaaca ttatcccgac cgggaggatt catgacattt aaacactacg atgtggtcag
      541 ggcggcgtcg ccgtcagacc ttgcggagcg actgactcaa aaactgaagg aggggtggca
      601 gccatttggc agtcctgtcg ccatcacgcc ttataccctg atgcaggcca ttgcggcgga
      661 aggtgatgtc accacacctg tgctggtgaa gccgtcggat ggagaaggca cagttatcag
      721 caccaccagc gaaccggagt attactttgt tgttgttctg gcggggcagt caaacagcat
      781 ggcatatggt gaaggccttc cgctgccgga gacatatgac cgtccggacc cgcgtattaa
      841 gcagctggcg cgccgcagta cggtgacacc gggcggtgcc gcctgtaagt ataacgacat
      901 cattccggcg gaccattgtc tgcatgatgt gcaggacatg agccggctga atcatccgaa
      961 agcggacctg tcaaaggggc agtacgg
//
LOCUS       DAZGBC010000505          983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGBC010000505 DAZGBC010000000
VERSION     DAZGBC010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.509"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..732
                     /locus_tag="ACHK1J_005483"
     CDS             <1..732
                     /locus_tag="ACHK1J_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914580.1"
                     /translation="DEFSSLLADTERQVLEGSSFVLCCGEKINGTISELLRKKITDST
                     HSIKSLTLSESPSYEVYEEIFKKAVFTPDECHLVRDSLVQDGIKPEKIAKIISCLSSR
                     TGIGNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSLVALNGRSYHQHVDFSE
                     YGFTQTLCMLGAVVNNANDTFDVANYIYTTKGENIIDNDAEDDSPYVHSDESYRHKDD
                     YWARDNDVRNLRRNFSGNITIATYK"
     gene            894..>983
                     /locus_tag="ACHK1J_005484"
     CDS             894..>983
                     /locus_tag="ACHK1J_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="response regulator in two-component regulatory
                     system wtih UhpB; phosphorylated UhpA is a positive
                     activator uhpT, a hexose phosphates transporter; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO7914581.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVV"
BASE COUNT          292 a          203 c          192 g          296 t
ORIGIN      
        1 gatgaattta gctcactcct tgcagataca gaaagacaag ttctggaggg aagttcattt
       61 gttttatgtt gtggtgaaaa aataaacggc actataagtg aattacttcg caaaaaaatt
      121 acagattcaa cacattccat caaatctctc actctctctg aatctccttc ttatgaagtt
      181 tatgaggaaa tattcaagaa agctgttttc acacctgatg aatgtcatct ggtgagagat
      241 tctttagtgc aggatggtat aaagccagaa aaaatagcaa aaattatttc ttgtttatcc
      301 agtcgtaccg ggattggaaa tttgttttat gcctgttcac aagcatttca tgttgctaat
      361 gataacgctg cacttgatgt gcgtcgcgcc gctacatctg ttggcgatac aatagatgtt
      421 tgtccgagtt tggttgcttt gaatggtaga agttaccatc agcatgtcga tttctcagaa
      481 tacggattca cacaaaccct ttgcatgtta ggagcagtcg taaataacgc gaatgacacc
      541 tttgatgttg ctaattatat ctataccact aagggcgaaa atattatcga taacgacgct
      601 gaagatgatt ctccctatgt acattccgat gaaagttata gacacaaaga tgattattgg
      661 gcaagggata atgatgtccg caatctaagg agaaatttct caggcaatat tacgattgca
      721 acctacaaat aattcgcttt ttctcaataa cacgcaccaa acgcttacct ttcaggtaag
      781 cgttttgacg ataagcccaa catcattcag ccaatacata ccccatatcc aacttgaaca
      841 acatcgcgct tatcgttaag gtatgcgcgt tttttatccg ccaggaccca atcatgatca
      901 ccgttgccct tatagacgat caccttatcg tccgctccgg ctttgcgcag ttgctggggc
      961 tggagcctga tttgcaagta gtt
//
LOCUS       DAZGBC010000506          976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGBC010000506 DAZGBC010000000
VERSION     DAZGBC010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.510"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..276
                     /gene="traQ"
                     /locus_tag="ACHK1J_005485"
     CDS             <1..276
                     /gene="traQ"
                     /locus_tag="ACHK1J_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO7914582.1"
                     /translation="DSFGPIAYVQPTTFGAAAGAANAMLSLVKLAGVGFAMNGISIWR
                     KSLLDGHTALSSSESISKGNVKFVAGVLLVFIDRVIDASLASLGLAF"
     gene            325..723
                     /locus_tag="ACHK1J_005486"
     CDS             325..723
                     /locus_tag="ACHK1J_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO7914583.1"
                     /translation="MSRFRAMTARLCAAFIYVQLYLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            780..>976
                     /locus_tag="ACHK1J_005487"
     CDS             780..>976
                     /locus_tag="ACHK1J_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO7914584.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPG"
BASE COUNT          208 a          279 c          244 g          245 t
ORIGIN      
        1 gattccttcg gccctatcgc ttatgtacaa ccaaccacct ttggtgccgc tgccggtgcc
       61 gccaatgcca tgctctcact ggtaaaactg gccggagtgg ggtttgccat gaacggcatt
      121 tccatctggc gtaaatccct gctggacggg cataccgccc tgagcagcag tgagtccatc
      181 agcaaaggaa acgtcaagtt tgttgccggc gttctgctgg tttttatcga tcgcgtcatc
      241 gatgcctctc tggcgagtct tggtctcgcc ttctgatcca cattcatttt cagtaaatca
      301 gtctgttagt taaggagaca ctatatgtca cgattccgtg ccatgacagc acgtctgtgc
      361 gctgcattta tttatgttca gttatacctg gtggcctgcc gtgaccggct gatacgcctg
      421 ttacttctgc ttcccgtgat actgctgccc cacacagcga tggcaaacac cctcttcgat
      481 atgatcaaaa ccgttggtga tggtgcaaaa ggctccagca gttcatttgt ggctatcggt
      541 caggttattg gcctggtgct ctttatcggc ggcctgcttg gcctcaaaca aatcggtaaa
      601 aaacccggag cagggctggc ctgcctgctg tcaattctgg tcggcctctg ccttgccgtc
      661 gtaccggaac tgatgggcag cggacagaaa acgctcggtc tttctccggt ttcaatcagt
      721 taatccgcaa aggcaccggg aaaccggtgc cgtcttcaca caatgaagag gaccagacca
      781 tgtcctgtga gctgccccct ttcaccctgc tcagaacaga gcacggcaac gtgattgccc
      841 gcgacaaccg ggataatcag gagtttgtgt ttgcagagtg ttattccacc gccatcgcag
      901 cactgattct ggctgccgtc accctgtata cagagagaaa aacgtatgaa caaaacaccg
      961 ccactcatcg ccccgg
//
LOCUS       DAZGBC010000507          975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGBC010000507 DAZGBC010000000
VERSION     DAZGBC010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.511"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..791)
                     /gene="fucP"
                     /locus_tag="ACHK1J_005488"
     CDS             complement(<1..791)
                     /gene="fucP"
                     /locus_tag="ACHK1J_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO7914585.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAV"
BASE COUNT          295 a          210 c          219 g          251 t
ORIGIN      
        1 actgcccagc gccagtggcg aatacgcgcc aggcgagaaa gcgatgcgga gaacgatcct
       61 tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc
      121 agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc
      181 aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat
      241 tgatgtggca cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca
      301 aacgagttaa atgtttgcgc aagatttaag cggaagtgac cactactttc cggccctaat
      361 accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc aataataaat
      421 aggccaacta aaaataaggt gtagttcatt atttctgccg cgggccagaa taatgcagca
      481 cccaaggcat ataaaaataa cccggtaata atccctgctt tataactgag ttttttcatc
      541 aatatcccag caggaattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg
      601 cctgcctgga aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta
      661 aggttattgg ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg
      721 taacttctgc tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat
      781 gtgtttccca tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc
      841 aaaagtgcaa aaccagcgag ttcgggcttc atatcagtga atatttgcct gtctgaaatg
      901 aactcatgga ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat
      961 tagctggaag gtgac
//
LOCUS       DAZGBC010000508          974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGBC010000508 DAZGBC010000000
VERSION     DAZGBC010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.512"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..276
                     /gene="traQ"
                     /locus_tag="ACHK1J_005489"
     CDS             <1..276
                     /gene="traQ"
                     /locus_tag="ACHK1J_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO7914586.1"
                     /translation="DSFGPIAYVQPTTFGAAAGAANAMLSLVKLAGVGFAMNGISIWR
                     KSLLDGHTALSSSESISKGNVKFVAGVLLVFIDRVIDASLASLGLAF"
     gene            323..721
                     /locus_tag="ACHK1J_005490"
     CDS             323..721
                     /locus_tag="ACHK1J_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO7914587.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIAQVIGLVLFIAGLLGLKQIGKKPGAGLACIL
                     SIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            778..>974
                     /locus_tag="ACHK1J_005491"
     CDS             778..>974
                     /locus_tag="ACHK1J_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO7914588.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPG"
BASE COUNT          203 a          279 c          243 g          249 t
ORIGIN      
        1 gattccttcg gccctatcgc ttatgtacaa ccaaccacct ttggtgccgc tgccggtgcc
       61 gccaatgcca tgctctcact ggtaaaactg gccggagtgg gatttgccat gaacggcatt
      121 tccatctggc gtaaatccct gctggacggg cataccgccc tgagcagcag tgagtccatc
      181 agcaaaggaa acgtcaagtt tgttgccggc gttctgctgg tttttatcga tcgcgtcatc
      241 gatgcctctc tggcgagtct tggtctcgcc ttctgatcca cattcatttt cagtaattca
      301 gtctgttagt taaggagact atatgtcacg attccgtgcc atggcggcgc gtctgtgcgc
      361 tgcatttatt tatgttcagt tatgcctggt ggcctgccgt gaccggctga tacgcctgtt
      421 acttctgctt cccgtgatac tgctgcccca cacagcgatg gcaaacaccc tattcgatat
      481 gatcaaaacc gttggcgatg gtgcaaaagg ctccagcagt tcatttgtgg ccatcgctca
      541 ggttattggc ctggtgctct ttattgccgg cctgcttggc ctcaaacaaa tcggtaaaaa
      601 acccggagca gggctggcct gcatactgtc cattgtggtc ggtgcctgtc ttgccgtcgt
      661 accggaaatg atgagcagtg gacagaaaac gctcggtctt tctccggtct ccatcagtta
      721 atccacaacg gcaccggttt cccggtgccg tcttcacaca atgaagagga ccagaccatg
      781 tcctgtgagc tgcccccttt caccctgctc agaacagagc acggcaacgt gattgcccgc
      841 gacaaccggg ataatcagga gtttgtgttt gcagagtgtt attccaccgc catcgcagca
      901 ctgattctgg ctgccgtcac cctgtataca gagagaaaaa cgtatgaaca aaacaccgcc
      961 actcatcgcc ccgg
//
LOCUS       DAZGBC010000509          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGBC010000509 DAZGBC010000000
VERSION     DAZGBC010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.513"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..290)
                     /locus_tag="ACHK1J_005492"
     CDS             complement(<1..290)
                     /locus_tag="ACHK1J_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7914589.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDF"
     gene            complement(482..760)
                     /locus_tag="ACHK1J_005493"
     CDS             complement(482..760)
                     /locus_tag="ACHK1J_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914590.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
BASE COUNT          252 a          265 c          210 g          241 t
ORIGIN      
        1 tggaaatccg gatgttcctc aaacagcgcc agactggaaa aagccaccgc atccctgaag
       61 tacaccgacg atggttcatc tgtaataagt tgtctgatca gacattccgc cagcacggac
      121 atctgtggcc ggatctgtaa aagcaaagac gacgggctga ctgccatacc ggataatggc
      181 gtgcagaacc gcagataccg cctcaccacc tcctcactga ggtcagcgat aaggaaacgt
      241 ccggtatgag cctgaaaagc agggcgcaga tgcccggcaa tcagaagcat tgtgccggca
      301 ggtatgacag agttcctgtg ctccagacaa agcattgtct gtttgagagc cataatgaca
      361 acgcaggtac agggcatcgg atatccggta aatatgttga tttattaaag agacagacat
      421 gccccgtata taaaattccg tgtcaccggg tgctgaaaaa aactgcaccg acggcgctct
      481 tttactcctc cgttacggat atttcagccc ccttttcagg agcggtatcc ttcacctcgc
      541 tgagcccgga ctgagccgct tcagaaacct ctgccacctt tccggcagcc tgcattttca
      601 cttcctccgc ggcatccttc acatcactga gttgagtctg cgcagcttcc tttatatctg
      661 ccgccttctt acttacattc tcctttgtcg catcatcaca gcccgcaacc agaaatggtg
      721 cggaaataca taacgccaga attgtttttt cagcatacaa tctctcatta ttaagtgtta
      781 acgcagaaca gcttatattt cgtccccggg aaatggtcaa aagacaacca gaacatcaca
      841 gtgtcagggc acactcccgg tatatgcttt tataaccata acatgctgtt cctgatatta
      901 ccggaaatgt gcccactaca tttaacctgt tttcagcacc gggtcatcgg ctgtgctgta
      961 ttttttat
//
LOCUS       DAZGBC010000510          965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGBC010000510 DAZGBC010000000
VERSION     DAZGBC010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.514"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..268
                     /locus_tag="ACHK1J_005494"
     CDS             <1..268
                     /locus_tag="ACHK1J_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO7914591.1"
                     /translation="APEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHY
                     RALAHAWSLGLKKYPAVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            265..>965
                     /locus_tag="ACHK1J_005495"
     CDS             265..>965
                     /locus_tag="ACHK1J_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO7914592.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQNCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVA"
BASE COUNT          219 a          243 c          290 g          213 t
ORIGIN      
        1 tgctcctgag cagacgcagg atacattttt cggggtactt cccgcagagc ccgcagaagc
       61 atctgcccgt gtcatggaac ggatgcagtc gcctgaatgg cagtcgtttc aggcagggct
      121 tgctgaacac taccgtgcat tagcacatgc ctggtccctt ggactgaaaa aatatccggc
      181 tgtagttttt gatgacagtg aagtggtcta cggaaccaca gacgtggtgc tggcagaaca
      241 actgcgtaca gggggagggc agccatgatt tcctgcacat cacgtcatcg cgccggtgtt
      301 ctgctgttaa gtatggcggt attgccggtg gcagaggcat cgctgaacac cgcgcagata
      361 gtggccagtg cagcctcaca gaactgtatc agctggcggg tcagcgggat ttgctactgg
      421 ctgttctgca ccaaattcag ttgcaaggtc agaacatcag tgaaagtttc ccattatatc
      481 ccggaagtgg ttgtttccac ctataccgca cctggcggaa atccctggcg tgaaatgtcg
      541 gcggtcagca gtacagccgg tggtccggag aatgccataa gcaaaggtat tgccggtctg
      601 tcagcaggag gagggaaccc cgcagacatg aaggtacagg gggaacgtcg ttcgtcaatc
      661 cgttttaaat atgccgatgc aacagggcac ccggccacca cgcttatcgg agggatggtg
      721 ccgggatatt cctgtgagtc accggtgaca ccttttgttc cttattttct cagtacgctt
      781 gacagtattg cctggcgtac aggactaccg gagtcactgt acccggaggc cctgatcccc
      841 ggtaaacgcg aaataggcag ccaggaagaa gagaatatgt ggggcaatgt gtatccccgc
      901 tccgggttcg tgacccagac agatgatgat aaagctgctg cggtggtggc ccagcgggtg
      961 gctga
//
LOCUS       DAZGBC010000511          964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGBC010000511 DAZGBC010000000
VERSION     DAZGBC010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.515"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..295
                     /locus_tag="ACHK1J_005496"
     CDS             <1..295
                     /locus_tag="ACHK1J_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO7914593.1"
                     /translation="HLIRSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPR
                     KNIRQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPR"
     gene            556..>964
                     /locus_tag="ACHK1J_005497"
     CDS             556..>964
                     /locus_tag="ACHK1J_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914594.1"
                     /translation="MKIGTVAGTNDSITTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     NNSYQFIVTINSKNYGCPLDDTDINWCSIVYLLN"
BASE COUNT          321 a          153 c          191 g          299 t
ORIGIN      
        1 ccatctaatt agaagcaata ctatggcacc cacacctgta tatgctgatt atttaaataa
       61 cactactgat ttcagaccag atcctgaaga tatcttcaaa tgtggtcaga cgctcgaatg
      121 tggcgacccc cgcaaaaaca ttagacaaaa atatggccat catgggttag gggtaaatca
      181 gaatcaacaa tttgataatg gtgttatggg gctaaattcc aggatggcag tgtttgatga
      241 aaataaaaaa ataataggtt cttcctcagg gcttagttgt tttcaacctc gttaaaactc
      301 catgagtgct gtggtatgtt ttttaatcct gccagtattc cataggtttt cttaatcgat
      361 tgtagaggta tgtactgatg acgcctctac aatctttagg gactttctct actatgagat
      421 aagaaatttc atattatgct ttaaggctga atgatttgtg caaaatataa ttatttttcc
      481 atcgaacatg aggtgtatgt cataagatta aatgataact atgtattcct cgcggtgaat
      541 tatatgaggt aaaagatgaa aattggaact gtggcaggta ctaacgacag tataacgaca
      601 atcgcaacaa atgatatggt gcaagagcat gttaccaact tcactaaaga actgtttggg
      661 tatattgcaa atggtatagg tgatgatata tcaagtattg ccaggacaat gctcggtgag
      721 gttgtggaaa aaatagatga ttggcaaata gaacggttcc aacaatctat tcaagatgac
      781 aaaatatcat ttaccataca aacggatcat tcagaaaaat atagtatgct atcgggcatg
      841 cgtgcccata ttctacgtag gaataatagt tatcagttta ttgtgacaat taatagtaaa
      901 aattacggtt gccctcttga tgataccgat attaattggt gcagcattgt ttatttactt
      961 aata
//
LOCUS       DAZGBC010000512          964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGBC010000512 DAZGBC010000000
VERSION     DAZGBC010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.516"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..440
                     /locus_tag="ACHK1J_005498"
     CDS             <1..440
                     /locus_tag="ACHK1J_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO7914595.1"
                     /translation="PSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMPDGDALAGV
                     IAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISLPSSGTAPSVP
                     PPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            437..>964
                     /gene="traP"
                     /locus_tag="ACHK1J_005499"
     CDS             437..>964
                     /gene="traP"
                     /locus_tag="ACHK1J_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO7914596.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     AAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVAPQSDMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQS"
BASE COUNT          233 a          287 c          282 g          162 t
ORIGIN      
        1 tgccgtccgt gctcggtggg gtgggcagtg tcgggagtct ttacaaagac gccaacacac
       61 aggtcattca gggaaactac ggcagcatta ccggcagggt gggaatgcct gatggcgatg
      121 cgctggctgg cgtcattgca ggcggcatgg ccgaacgggg cagtcagttg ctgacccgcc
      181 aggcggagag cgaaccttac cggcaggtga cagtgtcacg cggagaggtt gtctccattc
      241 tgtttgttga gccggtcatg agcaacgata tcagtctccc ctcctccggc actgcccctt
      301 ctgtgccacc gccctccgga cgcccgctca cccaggaaga agcagagcgt caggcgtctg
      361 acaggatcca ggcagcagtc atgcgtaaac agaacgaaat gcggcaacgt tacggaatac
      421 aacaggaaag tcatccatga aaaacgaaca tgaagatccc ggcagttttg gcgaaacaac
      481 gacaccggca tccggcaaat ccttcctgaa acgccagtat atgggataca gcctgccagt
      541 ctgggccggc tgtgtcctca tcatcctggc cgcagtctgg tacaagtttc tgcggacacc
      601 ctcagagccc cctgcagatg caggcttcgc ggtcacaccg cccgctgctg aaagctgggc
      661 agagcccatg cccatgacgg agaccgggag cggaaatacc atgtccgcgc ctcctgtcgc
      721 cccacagtca gacatgcagc agcttgcccg tgacctgaaa gcagagctgg acagacggga
      781 tgaggaaatc cgacgtaccc tgaccatgct gaaagagagc atcacaaccc ttggcgacac
      841 catacggaaa gatgaggcct gggcacagga aacccgtcag ttgcttgatg aaacccgtaa
      901 gcaggtgcat gccctgcaga tgcagggacc actgcaaatc ccgaccggga ataacggcca
      961 gtca
//
LOCUS       DAZGBC010000513          955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGBC010000513 DAZGBC010000000
VERSION     DAZGBC010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.517"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..189
                     /locus_tag="ACHK1J_005500"
     CDS             <1..189
                     /locus_tag="ACHK1J_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO7914597.1"
                     /translation="ARLRGQQFSYSGVRLPLPVSLHVRDMTFADVLRLTEAQTAWRAT
                     LSQSAGRLHLSFMTPEKK"
     gene            186..>955
                     /locus_tag="ACHK1J_005501"
     CDS             186..>955
                     /locus_tag="ACHK1J_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO7914598.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFT"
BASE COUNT          216 a          295 c          253 g          191 t
ORIGIN      
        1 gcccgtctgc gcggccagca gttcagctac agcggcgtcc gtctcccctt acccgtctca
       61 ctgcacgtcc gcgacatgac ctttgctgat gtccttcgtc tcactgaagc ccagactgcc
      121 tggcgggcaa cactctcgca gtcagccggg cgtttacatc tgtcttttat gacgccggag
      181 aaaaaatgaa aacacacctg tgtctcctgc tgtctgctct cctgcttaca ggctgtgccg
      241 gacagcatca ccgttcttct gccgtcatga cagaaaacgc accgcccccg gatatccgtg
      301 catggctgac gccctccggc aaccaggatg atggtatttc agatacccgt cgtcagatgc
      361 tcacggatgc cggcagaacg ctgggattcc gtggtggaaa agcacagcgc tcatgggaac
      421 tgattcaggc actgaatgcc cgggaaagta ccctgaatgc cctgtatgat ttccggcctc
      481 tcatcagcaa ggaaggctgg ctgcctcctg ttatcgatga agctcaggat gttgcacaca
      541 tcagaccgga ccagataaga acagcctccc gtgtctggac tatcctccgc ccggaacgct
      601 ttgtcagtaa tccacctgga tggcgtgact ggctgctgaa aggactctca accaccgcca
      661 caccaggcac tgaaggccgg gttgtgccgg aagaccgggc acaacgcaga ctatgggaaa
      721 acgccttacg tcagggatgg caggaagggc gcctcaacgc agatctcaca ctggaagcta
      781 accagaagcg cctgacccgt gattaccggg gcatgatgct ttacgccctt ctgtggcgtc
      841 agggcatgat cacacgtcca gatgtcacag agcagatgca gacggttacc ggcaacgggc
      901 aaaaactcat cactggcgat cgcgtccgcc gcctgaaaac acatgctgaa tttac
//
LOCUS       DAZGBC010000514          944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGBC010000514 DAZGBC010000000
VERSION     DAZGBC010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.518"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>944
                     /gene="traD"
                     /locus_tag="ACHK1J_005502"
     CDS             <1..>944
                     /gene="traD"
                     /locus_tag="ACHK1J_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HFO7914599.1"
                     /translation="ASRVAGQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLI
                     MRYVNNIADLYIRYAGKVISEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAI
                     EVALSSEAGKKLYDPILDGLRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDY
                     LDMSDTRPVFDWEQVIRKKAVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGM
                     NAGLPGGNGTKNPVNLHCDEFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTG
                     SAAKTAQVQGNFNNMIMLRVRENRTAELLTTQLSQVEI"
BASE COUNT          222 a          207 c          288 g          227 t
ORIGIN      
        1 gcttcccgtg tggccggtca gttatccggt gaaggtaaca gtgcggcctt ccgtgagttt
       61 gcatggcggt ttgtcaacat tgttgcccgt gcactggtgg cactggggga gcgcccggat
      121 tacacgctga ttatgcgtta tgtgaataat attgccgacc tttatatccg ttatgctggt
      181 aaggtgatca gcgaacagat gccggaactg gaaaacgcca ttctgaataa catgaatgta
      241 ctgggggaag cggatgttcc ccggaccatg cagaaccagc cggatgctgt gcgtatctgg
      301 gccattgagg tggctctcag cagtgaagcc ggaaagaaac tctatgatcc gatactggat
      361 ggtctgcgaa gcgcggtacg ttatgatcgt acctattttg acaaaattgt ggcatccctg
      421 ttgccgttgc tggaaaaact gaccacagga aagacggcag aactgctggc acctgactac
      481 ctggatatga gcgatacccg tcccgtgttt gactgggagc aggtgatacg taaaaaggcg
      541 gtggtgtaca ttggtcttga tgcgctgagt gacagtgagg tggccagtgc cgtgggtaac
      601 tccatgtttg cggatctggt gagtgtcgcc gggcatatct acaaacacgg aatgaatgcc
      661 ggtcttccgg gagggaatgg taccaaaaat ccggttaacc tgcattgtga tgagtttaat
      721 gaactgatgg gcgatgagtt cattccgtta atcaacaagg gaggcggtgc cggtatgcag
      781 gtgacggctt acacgcagac ctcttctgat attgaggccc gtaccggtag tgctgccaaa
      841 acggcccagg tgcagggtaa ctttaataac atgattatgc tgcgggtcag ggagaaccgg
      901 acggccgagt tactgacgac ccagttgtcc caggtggaaa ttta
//
LOCUS       DAZGBC010000515          938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGBC010000515 DAZGBC010000000
VERSION     DAZGBC010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.519"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..610
                     /locus_tag="ACHK1J_005503"
     CDS             <1..610
                     /locus_tag="ACHK1J_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7914600.1"
                     /translation="RIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYHL"
     gene            610..>938
                     /locus_tag="ACHK1J_005504"
     CDS             610..>938
                     /locus_tag="ACHK1J_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO7914601.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMS"
BASE COUNT          201 a          299 c          255 g          183 t
ORIGIN      
        1 ccgcattgaa cagcttgatc aggaggtcaa gaccaaagtc tatgacgaac tgcacaatgc
       61 aaaagggatc aatttcatct gggaagccct ggacacccag gagctggagc agcgtaagtt
      121 cgggatacgc acagtgctga gcacccagct actgcaggac tatccaccgg cagttctcaa
      181 atccgccaac accctctggt tgctgcgtta ccgccctgac gaaattccgt tcctgcggga
      241 taattttggt gtccctgaag tcaccctgcg gcgcttcctg aaaatgccgg aaggggccgc
      301 accggacggc agcggtgtcc cggtactggc tgtgttccgt gtaaaaaacg gcacgctggc
      361 ccggatcctg aaattcaccc tcggtccact ggagctgtgg gcactgaact cctctcctaa
      421 agacagcgcc ctgcgacgtg cgctgacgca ggaggtgggg tcactgcggg cacgccagat
      481 cctggccgaa cactttcccc ggggttcagc cacctcactg atagaacacc gcgcccgcac
      541 acatgacagt gaaaacgtca tccatgagct ggccgcagaa ctcatccgta agcagggata
      601 tcacctctga tgtacagcat tcatatcacc ccccgctgcc ggcgtcctgc ccggggacag
      661 gtcttcctga aaacgctgcg cgtggacggt gacagtttca gcttcaccct gccgtccgcc
      721 tgcttcacac tgcgaccgtc ccccaatccc cgggtcattt ttgccagtga cagacgggct
      781 gacggggaga tgagtggtat tcgcctggcc ctgcccggag agacaggcac gctgataaca
      841 acggccacct ggcaggttaa cgtgccggag cgaccgcctt ttgatataca gcatgtcatt
      901 cactggcttc tgcccgcacc actcactgcg ctgatgtc
//
LOCUS       DAZGBC010000516          938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGBC010000516 DAZGBC010000000
VERSION     DAZGBC010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.520"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..610
                     /locus_tag="ACHK1J_005505"
     CDS             <1..610
                     /locus_tag="ACHK1J_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7914602.1"
                     /translation="RIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            610..>938
                     /locus_tag="ACHK1J_005506"
     CDS             610..>938
                     /locus_tag="ACHK1J_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO7914603.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMS"
BASE COUNT          201 a          294 c          252 g          191 t
ORIGIN      
        1 ccgcattgaa cagcttgatc aggaggtcaa gaccaaagtc tatgacgaac tgcacaatgc
       61 aaaagggatc aatttcatct gggaagccct ggacacccag gagctggagc agcgtaagtt
      121 cgggatacgc acagtgctga gcacccagct actgcaggac tatccaccgg cagttctcaa
      181 atccgccaac accctctggt tgctgcgtta ccgccctgac gaaattccgt ttctgcggga
      241 taattttggt gtccctgaag tcaccctgcg gcgcttcctg aaaatgccgg aaggggctgc
      301 accggatggc agcggtgtcc cggtactggc tgtgttccgt gtaaaaaacg gtacgctggc
      361 ccggatcctg aaattcactc tcggtccact ggagctgtgg gcactgaatt cctcacccaa
      421 agacagcgcc ctgagacgtg cgctgacgca ggagctcggg tcactgcggg cacgccagat
      481 cctggcagaa aactttcccc gtggttcagc cacctcactg atagaacacc gcgcccgtac
      541 acatgacagt gaaaacgtca tccatgatct ggctgcagaa ctcatccgta agcagggata
      601 tcacctctga tgtacagcat acacatcact ccccgctgcc ggcgtcctgc acggggacag
      661 gtcttcctga aaacgctgcg cgtggacggt gacagtttca gcttcaccct gccgtccgcc
      721 tgcttcacgc tgcgtccctc ccccaatccc cgggtcattt tcgccagtga cagacgggct
      781 gacggggaaa tgagtggcgt tcgcctggct ctgcccggag agacaggcac gctgattaca
      841 acggccacct ggcaggtcaa cgtgccggag cggcctcctt ttgatataca gcatgtcatt
      901 cactggctcc tgccctcgcc tctcacggcg ctgatgtc
//
LOCUS       DAZGBC010000517          928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGBC010000517 DAZGBC010000000
VERSION     DAZGBC010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.521"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..>928
                     /gene="hlyE"
                     /locus_tag="ACHK1J_005507"
     CDS             23..>928
                     /gene="hlyE"
                     /locus_tag="ACHK1J_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO7914604.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPE"
BASE COUNT          314 a          176 c          206 g          232 t
ORIGIN      
        1 atatttaaag aggcgaatga ttatgactga aatcgttgca gataaaacgg tagaggtagt
       61 taaaaacgca atcgaaaccg cagatggagc attggatctt tataataaat atctcgatca
      121 ggtcatcccc tggcagacct ttgatgaaac cataaaagag ttaagtcgct ttaaacagga
      181 gtattcacag gcagcctccg ttttagttgg cgatattaaa accttactta tggatagcca
      241 ggataagtat tttgaagcaa cccaaacagt gtatgaatgg tgtggtgttg cgacgcaatt
      301 gctcgcagcg tatattttgc tatttgatga gtacaatgag aagaaagcat ccgcccagaa
      361 agacattctc attaaggtac tggatgacgg catcacgaag ctgaatgaag cgcaaaaatc
      421 cctgctggta agctcacaaa gtttcaacaa cgcttccggg aaactgctgg cgttagatag
      481 ccagttaacc aatgattttt cagaaaaaag cagctatttc cagtcacagg tagataaaat
      541 caggaaggaa gcgtatgccg gtgccgcagc cggtgtcgtc gccggtccat ttggattaat
      601 catttcctat tctattgccg cgggcgtagt tgaagggaaa ctgattccag aattgaagaa
      661 caagttaaaa tctgtgcaga gtttctttac caccctgtct aacacggtta aacaagcgaa
      721 taaagatatc gatgccgcca aattgaaatt aaccaccgaa atagccgcca tcggggagat
      781 aaaaacggaa actgaaacaa ccagattcta tgttgattat gatgatttaa tgctttcttt
      841 gctaaaagaa gcggcaaaaa aaatgattaa cacctgtaat gagtatcaga aaagacacgg
      901 taaaaagaca ctctttgagg tacctgaa
//
LOCUS       DAZGBC010000518          928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGBC010000518 DAZGBC010000000
VERSION     DAZGBC010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.522"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..851
                     /locus_tag="ACHK1J_005508"
     CDS             51..851
                     /locus_tag="ACHK1J_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914605.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKAYVSMVKDLVSRFQ"
BASE COUNT          296 a          177 c          210 g          245 t
ORIGIN      
        1 atcaatgcta aatattctaa tcattatgac aggcgaggga gtgtccaatt atgaattcaa
       61 aaaagctttg ttgcatatgt gtgttattct cgctgcttgc aggatgtgcc tctgaatctt
      121 ctattgatga aaagaagaaa aaagctcaag tcacacaaag taatattaat aaaaacactc
      181 cccagcaact gacagacaaa gatttattcg gtaatgaaac cactctggcc gtatccgaag
      241 aggatattca agcagcgctt gatggagatg agttccgcgt tccccttaat tctcctgtaa
      301 tacttgttca atccggcaac cgcgcaccgg aaaccattat gcaggaggag atgcgaaaat
      361 attatactgt ttccacattc tctggtatcc cggacaggca aaagcctctg acttgtaaca
      421 aaaacaaaga taaaaatgag aacgaggatg ttgcccgtgc tgagaatatg aactggatgc
      481 aggcactgcg ttttgtggct gcaaaaggac atcagaaagc gattattgtt taccaggata
      541 tgttgcagac aggaaaatat gactctgcgc tgaaatcaac agtatggtcc gactataaaa
      601 atgaaaaact cactgacgct atctctctgc gctacctggt acgtttcacg ctggtggatg
      661 tggcaacagg tgagtgggct acctggtcgc cggtgaatta tgaatcgaga gtaatttttc
      721 cgccggctgg aattacaaaa acgagcaata aagaactatc aaatgaccat gttacggaag
      781 cgcaactgtt taatttgaaa caaaaagcat atgtatcgat ggtgaaagac ttggtgagtc
      841 gattccagta atcgttattg ttatgctgcc gcctgtagtt tagggtggca gcaaacagga
      901 gttgagtatt tctataatgt aaatatga
//
LOCUS       DAZGBC010000519          914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGBC010000519 DAZGBC010000000
VERSION     DAZGBC010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.523"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..242
                     /locus_tag="ACHK1J_005509"
     CDS             <1..242
                     /locus_tag="ACHK1J_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO7914606.1"
                     /translation="RGIIQDEQDLLDNPCLNIKIGTEILYNHFSRCGVTWQCLGTYNA
                     GFAMDNKKKRLQYAKKIYIVYTRLNELDNRKALAK"
     gene            complement(260..892)
                     /locus_tag="ACHK1J_005510"
     CDS             complement(260..892)
                     /locus_tag="ACHK1J_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7914607.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGSELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          290 a          179 c          147 g          298 t
ORIGIN      
        1 aacgtggaat aatacaagat gaacaagatt tgttagataa cccctgtctg aacattaaaa
       61 taggaaccga gattttatat aaccattttt cacgttgtgg agtaacgtgg caatgcctgg
      121 ggacttataa tgcgggtttt gcaatggata acaaaaaaaa gagactgcaa tatgccaaaa
      181 aaatatacat tgtatatacc aggcttaatg aactagacaa tcgtaaggct ttagcaaaat
      241 gagcctaaag cctctttttt tatatagtgc aaacacccat acgtaaagct gttttgatca
      301 actcgatacc actgtgaaca tctaatttct tcatgatatt gagtctatga gtttcgactg
      361 ttttcctgct taactgcaag aaattagcga tttctttgtt tgttttacca tcagctatga
      421 gttgtaatat ttctgactca cgatttgtaa gttgattatc agaggaatac ctggaagata
      481 ttttttcaca atccatatgt acactatcaa aataagtgta gccattacta atcgaatcaa
      541 tagcgcgatt tagtttcgcc gggtgactgg ttttccatac gcatcctttc gcacctgctt
      601 caagcaattt tattgaatca atatacggca tctttgctac aaatgtaata atatgagcat
      661 ctattttatg cgatttaacc cattttacga gttcggaccc catcattcca cctgcaaatt
      721 ctcctaaaat aaatatttgg gctgcagatt tgttacactc cgctatagct ttcttcagat
      781 ctttatagcc cccaacaacc tcatataaat caggataatg tccgaggaat ccaattatcc
      841 catcaatcat aaacggctgc tgatctgaaa ctacaatttt aatttttccc atatattcat
      901 cctgagtgta aatg
//
LOCUS       DAZGBC010000520          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGBC010000520 DAZGBC010000000
VERSION     DAZGBC010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.524"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..252
                     /locus_tag="ACHK1J_005511"
     CDS             <1..252
                     /locus_tag="ACHK1J_005511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914608.1"
                     /translation="EGIDMNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQ
                     DKPQNMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            complement(303..>905)
                     /locus_tag="ACHK1J_005512"
     CDS             complement(303..>905)
                     /locus_tag="ACHK1J_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO7914609.1"
                     /translation="EVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAVARIAGTAG
                     SAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLITLTFVVFAV
                     HFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGTMFLVLPAV
                     WLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
BASE COUNT          253 a          262 c          172 g          218 t
ORIGIN      
        1 gagggtatag atatgaataa cgaatctgaa aaagcaatgt ggcgttgtaa gcctgtggat
       61 aatccatgga ccttcacttc cctgctgaaa aaagcagtct gtgccgtttt accagatttc
      121 acacctgctc aagataaacc ccaaaatatg ttttcactaa gtaatgaaaa agatactgat
      181 tattcagtac caatgagacc ctttgcatta tatgacccgt ggaaacatca ccatgacaat
      241 gctgatttct aagaaaagca agggtttccc caattatccc agctaaggaa accctaagaa
      301 ttttacttat tagctaaatc tccacttttt gctgctgcct tagatacatt acttgttcct
      361 ttgttaattg catcccccat aaccgttccg acacttaccc cagcccagga caacgctccc
      421 agccacactg ccggcaagac gagaaacatc gtccccatca ccagattcat tatcagatca
      481 tccgatgtgt tctggaatcc catcatattg aaccgggtat gtgtatccga actgtacagc
      541 gccgtcagca tccagctgtc aagccaccgt gcaagctccc accagaatgt cagaaagtgt
      601 accgcaaaca ccacaaacgt cagtgtgatt aacgtcgaca gttcatatgc cgcaaacgcc
      661 agaatcagcg gtatcatgat gtaaatcgcc atcagtatta tcgactgtac catcggcagt
      721 gcctgccgca ttgcatccag tgccggaaac gcacccagtg ctgctaccgc actgcccgcc
      781 gttcccgcaa tccgggccac cgcattcatg gtcgtcatat ctgcattgcc accatagcca
      841 cgatacacct ctcccccctg agagagcgtc aggtttgccg gactcaccag acgcttgatc
      901 acctc
//
LOCUS       DAZGBC010000521          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGBC010000521 DAZGBC010000000
VERSION     DAZGBC010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.525"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          257 a          226 c          177 g          240 t
ORIGIN      
        1 gtcagcatgt cttcatcctc atcagcagct cttcagtgga taacatccac agttacacct
       61 gcatttacgc gcaacaaaaa caccagatga agattttatt cattcagctc cgctaaaaat
      121 taatcttgcc gtggaatatc gggaaaacag gaagttacgt taaaagccga aggaaaaact
      181 cacaatccct tcttcaatca gcctctgaaa atcggctttt cagcatgtct tcccccattg
      241 ttcgtatacc ctgttcgatg gattattatt gatccttatg atcgcttcac gtaaacacga
      301 ttctggctcc acaccattct ggacgcgatt gttaccaaaa aagacaacca tcacaagacg
      361 tcacagacgg tgcgtgcgct tactcctgta tatctggcgc aacagaatct accagtgtaa
      421 actctctatg cttttgcgca gaagcagtgg gcctgggttc gcttatgtcc tgcatctaca
      481 agcgaatgac tgaccagaga ctcccatatc tggtcagtcg cccattttta ttttccctat
      541 acactcagac tacaatagcc gtaacaaaca gcataccccc agtcatagtg gcccctttat
      601 cttcttatgg cttgtctgtg gtactggaga tagagcaact gtgggagtac tgttatggcc
      661 cccggtaaga aaaactcttg cttaatgtac agcagaactg ttcccagaaa gaagcctgtg
      721 catattttca tagaaggaga tcacccataa cggcgttttt cacggtaata cacgccaggt
      781 aaagaatgct ggattatcaa tcaaagctcc gcacaggaag cgcttatgcc gtagagcgcc
      841 ctctggagaa aagtgctact tcttgtcgat tcagactagt tcaggaaatt atacgaaaat
//
LOCUS       DAZGBC010000522          898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGBC010000522 DAZGBC010000000
VERSION     DAZGBC010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.526"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>898
                     /locus_tag="ACHK1J_005513"
     CDS             <1..>898
                     /locus_tag="ACHK1J_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7914610.1"
                     /translation="LGKSVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDS
                     LPEQRKDEAVGIILSNDPEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQP
                     CNPGDTRQIISNLVDLAFREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAA
                     TWFEIRDMLHMAGNIPAAQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLL
                     DYIRRALDQGHSDYPMMSGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLA
                     GQIAGGDFVLPQYQEEIRKNLDP"
BASE COUNT          217 a          261 c          246 g          174 t
ORIGIN      
        1 ctgggcaaat cggtactcat caacgcactg tcggagatcc agatagcctc agcccagaag
       61 aacctgcctt tcattgccta tatcgacaag ggattcagtg cacagggact ggtacagctt
      121 atccgtgaca gcctgccgga gcagagaaag gacgaagccg tggggatcat tctgagcaat
      181 gatccggaac acacccgtaa cctgtttgat gtgatgtacg gtgctaaaaa acccgccacc
      241 ccggaaaaga acttcatggt atctgtactc tgcgcactgt gcgtggatac cggcaccggt
      301 cagccctgta atcccggtga cacccggcaa atcataagca acctggtgga tctggccttc
      361 agggagtatg gcgaaaacaa ccccagactg taccgtgcag gaacagagcc ccttgtggat
      421 ctcgccctgg aagagagtgg catcgcagaa caacatgacg ctggatggtg gaatgctgcc
      481 acctggtttg aaatcaggga catgctgcat atggctggca atatccccgc agcccagcgg
      541 gcacactacc aggccatgcc cctgctggct gaaatgtccg ccctgctggg acagccatcc
      601 atccgggatg tgttcggcac agttcagcgc gataactcag cagaattgtt actggactac
      661 atccgccgtg ccctcgatca gggacacagt gactacccga tgatgtccgg ctgtacccgg
      721 tttatgctca gccccgacac ccgcgtcgtg gccgttgatc tgaacaatgt ggccggtgac
      781 aagacacctg ccggacgtct gcgcaccggc atcatgtacc tgctggcagg acaaatcgcc
      841 ggcggggatt ttgtcctgcc gcagtatcag gaggaaatac ggaaaaatct cgacccac
//
LOCUS       DAZGBC010000523          894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGBC010000523 DAZGBC010000000
VERSION     DAZGBC010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.527"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..140)
                     /gene="lfpB"
                     /locus_tag="ACHK1J_005514"
                     /gene_synonym="stgB"
     CDS             complement(<1..140)
                     /gene="lfpB"
                     /locus_tag="ACHK1J_005514"
                     /gene_synonym="stgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone protein"
                     /protein_id="HFO7914611.1"
                     /translation="MPMYLLRLFFAVTLITQMSMAMAGIVVGGTRVIFYGDKPDTTIS
                     IF"
     gene            complement(243..845)
                     /gene="lpfA2"
                     /locus_tag="ACHK1J_005515"
     CDS             complement(243..845)
                     /gene="lpfA2"
                     /locus_tag="ACHK1J_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO7914612.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
BASE COUNT          282 a          193 c          169 g          250 t
ORIGIN      
        1 ttaaaaatag agattgtagt atcaggtttg tcaccataaa aaataacgcg agttccccca
       61 acaacaatac cagccatagc catcgacatc tgcgtaatca aagtaacagc aaaaaacaat
      121 ctaagtaaat acataggcat aaatgtatct cataaaaata taataatgac gcccttcttt
      181 taagtaaaag agaaccataa tggcgagcgc cattttattt aaggtattat gttaagaatg
      241 atttatttta tatattcaac tgtaaactgc gagtcggcgt tagctgtacc agggtcaata
      301 gtcgtttccg tagcaatata acgagccatg aacttcatgg atgcattacc attagaaatc
      361 ggagttgatg cagaattttc atataacttg acctgagtgt tatctgcacg gttatataaa
      421 cgaatagcta cgcctttcgc tgcactaacg gcacccgcac cagtataatc cccactgtta
      481 tcactcgggc taataccagc ggcagcatcg ttagagttat caagcggggt accgattgcc
      541 aggttgccat taccatgagc atcttcatta ccatcgaaac gaattgcggc ggacttaaag
      601 gtttccgggc attgagtcag atcgatagaa aaagccgtcg gggcagccac atcatcgacg
      661 ccactaaagg ctgtacggtt tactttgcct aagtcaacga cctgatcttt agtttcagga
      721 gtgacttcgc aagtactgtc aataatttca ccacggaaat ggactgttcc atcagccgcc
      781 tgtaggtgac ctgccataaa cacagaagtt aacgctaatg ctcctacaat catatttggt
      841 ttcataaata taactccatt aatttgatat tattcctgtg tgttatccat ttat
//
LOCUS       DAZGBC010000524          886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZGBC010000524 DAZGBC010000000
VERSION     DAZGBC010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.528"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..770
                     /locus_tag="ACHK1J_005516"
     CDS             147..770
                     /locus_tag="ACHK1J_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7914613.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRDTGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDTLTGTTRLVSAYAL"
BASE COUNT          216 a          182 c          252 g          236 t
ORIGIN      
        1 tatattttcc tatggtggtg gtgttaatat tacacgttga aaaaagtaca tgtttttatc
       61 ttctgaggca cttggcttca cggaggatat ttattcctgt aggctaatca ctgtgtctgg
      121 gtaaacaaca gatatctggt ggcgatatgc aggaacgtat tgattatcat attgagaaat
      181 atcagttcag ggccaggaat gagtctccac gactgatgcg tcagtgggct gatgtcatgc
      241 gtgaatgccg ggataccggg gcaggtgcca gggagcgact gcggacagca ttactgaatg
      301 ttgattatgt gaccagtttt gagctgccgt ttcgtctgct gttaacccgg acaccgcaga
      361 tgattgacac tctgcgtcgg gaatggctgc tcagtcagaa aaaagctctg ttcaacgggc
      421 ggcagcgggg atgtgtatac agtctgcaga atgacttatc cggcgtgccg gattgtttca
      481 gctacagtct tgcgacgcga atacggcgga tggactgcta tggcatgacg acacgttgtt
      541 tcacagatat cgccgggcag gttaaagccc cgacggcacg actaaaactg gcgctggaaa
      601 gcgggttgct ggtaacggca ctggatggcc tgttctggct gggaactcag cgaattgcag
      661 ctgatgttca gagactgcgt cagtcaggta tggtgatagt cacccgggaa gttgaggtgt
      721 cagataccct gaccggaacc accagactgg tttctgccta tgcactttaa agcactgggg
      781 gaatctttgt taccgtattc gtactgattc tgaagtttac cattgttgac cgggactgat
      841 tacagacagg atattttatg cgagataaat cagaagtatt aaccag
//
LOCUS       DAZGBC010000525          875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGBC010000525 DAZGBC010000000
VERSION     DAZGBC010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.529"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..254
                     /gene="ymgI"
                     /locus_tag="ACHK1J_005517"
     CDS             81..254
                     /gene="ymgI"
                     /locus_tag="ACHK1J_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO7914614.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            256..600
                     /locus_tag="ACHK1J_005518"
     CDS             256..600
                     /locus_tag="ACHK1J_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO7914615.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            604..>875
                     /locus_tag="ACHK1J_005519"
     CDS             604..>875
                     /locus_tag="ACHK1J_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO7914616.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCA"
BASE COUNT          257 a          167 c          215 g          236 t
ORIGIN      
        1 ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg
       61 aataaaaata ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa
      121 aaaacataat tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc
      181 gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa
      241 taccggacat ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc
      301 tgctccacgc cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca
      361 ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc
      421 gctggtgttg gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca
      481 aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg
      541 gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa
      601 ggaatgatga tgaaaaaatt tgctttactg gcgggtctgt ttgttttcgc accaatgact
      661 tgggcgcagg actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca
      721 gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg
      781 ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc
      841 aaagtggtcg aaaaactgaa tcaggtttgc gctaa
//
LOCUS       DAZGBC010000526          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGBC010000526 DAZGBC010000000
VERSION     DAZGBC010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.530"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..93
                     /locus_tag="ACHK1J_005520"
     CDS             <1..93
                     /locus_tag="ACHK1J_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914617.1"
                     /translation="LEWSSPTDDDELPEVTGPAWIVDKVAHYGG"
     gene            97..402
                     /locus_tag="ACHK1J_005521"
     CDS             97..402
                     /locus_tag="ACHK1J_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914618.1"
                     /translation="MTETGGQPPVSFPVKDVAVLLFLLHRLTRRGINAACGQCPASGR
                     ARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRTR"
     gene            478..>872
                     /locus_tag="ACHK1J_005522"
     CDS             478..>872
                     /locus_tag="ACHK1J_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO7914619.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWNRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLP"
BASE COUNT          168 a          259 c          253 g          192 t
ORIGIN      
        1 ctggaatggt cttccccgac agatgacgat gagctgccgg aagtcaccgg acctgcgtgg
       61 atagtcgaca aggtggcgca ttatggcgga tgaagtatga cggagacggg cgggcaaccg
      121 cccgtttctt ttccggtaaa ggatgtcgcc gtgctcctct ttttactgca ccggctgacg
      181 cggcgcggca taaacgctgc ctgtggtcag tgtcccgcgt ccggccgggc acgggacggg
      241 cgcttttacc gctcccggct ggcgtcggtg acagtgtacg ccagcctgtc gcccttttct
      301 gatgaacgcc cttcaagccg ctttcgcggc ataaccttgc cgtcaaacag acgacggctg
      361 cggtattcca cggtcggcct gacccgttac cggacgcggt gaacagccca caggcagcgg
      421 ggaacgggca ccacaggggt gccctcccgg tgccctctgt aagagaagga gttttgtatg
      481 tcccgttttg tccttggtaa ctgcattgat gttatggccc gtattcctga taacgccatt
      541 gatttcatcc ttaccgatcc tccatatctc gtcggttttc gtgaccgtca ggggcgcacc
      601 attgccggtg acgttaatga tgactggctg caacctgcca gcaatgaaat gtaccgcgta
      661 ctcaaaaaag acgcgctgat ggtgagtttc tatggctgga accgtattga ccgctttatg
      721 gcagcctgga aacgcgccgg attcagcgtg gcagggcacc tcgtgttcac caaaaactac
      781 acatccaaat ctgcgtatgt cgcgtaccgc cacgaatgcg cttacatcct ggcaaagggc
      841 agtccggccc ggccccgtaa cccgctgccg ga
//
LOCUS       DAZGBC010000527          867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGBC010000527 DAZGBC010000000
VERSION     DAZGBC010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.531"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..843
                     /locus_tag="ACHK1J_005523"
     CDS             127..843
                     /locus_tag="ACHK1J_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7914620.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          291 a          144 c          177 g          255 t
ORIGIN      
        1 attatgactt tagaatattt ttatttctaa tagacgagat ttttcctgtt atgatataat
       61 atgccgaatt aacacatgtt aacgatttac cagtaatgta aataaatttt cgaggagatc
      121 attccagtgg gacgtaaatg ggccaatatt gttgctaaaa aaacggctaa agacggtgca
      181 acgtctaaaa tttatgcaaa attcggtgta gaaatctatg ctgctgctaa acaaggtgaa
      241 cccgatccag aattaaacac atctttaaaa ttcgttattg aacgtgcaaa acaggcacaa
      301 gttccaaagc acgttattga taaagcaatt gataaagcca aaggcggcgg agatgaaacg
      361 ttcgtgcagg gacgttatga aggctttggt cctaatggct caatgattat cgccgagaca
      421 ttgacttcaa atgttaaccg tacgattgct aacgttcgca caattttcaa taaaaaaggc
      481 ggcaatatcg gagcggcagg ttctgtcagc tatatgtttg acaatacggg tgtgattgta
      541 tttaaaggaa cagaccctga ccatattttt gaaattttac ttgaagctga agttgatgtt
      601 cgtgatgtga ctgaagaaga aggtaacatt gttatttata ctgaacctac tgaccttcat
      661 aaaggaatcg cggctctaaa agcagctgga atcactgagt tctcaacaac agaattagaa
      721 atgattgctc aatcagaagt tgagctttcc ccagaagatt tagaaatctt tgaagggctt
      781 gttgatgccc ttgaagatga cgacgatgta caaaaagttt atcataacgt cgcaaatctc
      841 taattatatt ttaaagaaat ctgtctt
//
LOCUS       DAZGBC010000528          860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGBC010000528 DAZGBC010000000
VERSION     DAZGBC010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.532"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..149
                     /locus_tag="ACHK1J_005524"
     CDS             <1..149
                     /locus_tag="ACHK1J_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease"
                     /protein_id="HFO7914621.1"
                     /translation="YPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNSH
                     NNYK"
     gene            complement(450..>860)
                     /locus_tag="ACHK1J_005525"
     CDS             complement(450..>860)
                     /locus_tag="ACHK1J_005525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914622.1"
                     /translation="VPFARNRIFHASGDGGVCLPSFIRVISFYGLNAAQNQPAETVRA
                     AAATTGRFAVAFPVRVAPGGRSFMKTNAPRLFFVDKESGVSFDVFHVLSVSFPVTVVP
                     HMTGECNIQNLTENVPFVGTGNQSCIQGLCHNSG"
BASE COUNT          270 a          224 c          177 g          189 t
ORIGIN      
        1 tatatcctga tttcaacaca gattacaccc tgccggcata ccagagagaa gccagaacaa
       61 tgaaacgggg tctgtgggca gacaaaaaac ctgttccacc atggctgtgg cgaaaagaaa
      121 ataaaaatag ccacaataat tataaataag acatctcaca gaaatatatt tcctgccgtt
      181 ttatttcccc tgccagtccc caacgggaaa taacccacag aaattacaca gatgtgaata
      241 ataaacctgt tcgatgtatc cgtcacaaaa gagttgatgt ttgactcaga agaaaatacg
      301 cctgaagtta aggaaggctt cagcttcatg attgttctgc catgaacata tatccctctg
      361 tagcgccctg cctttcataa catttctgtt acagcaacaa cgaaagacaa cagcaatgtc
      421 ggccttttac gtccacatga atattccact tatccggaat tgtgacacag cccctgaata
      481 caggattgat tgccagtgcc cacaaatggc acattttcag taagattctg aatgttacat
      541 tcccccgtca tgtgtggcac aactgtcacc gggaaagaaa cggaaagcac atgaaaaaca
      601 tcaaaactca caccggactc cttatccaca aagaacaagc gcggcgcgtt cgtcttcatg
      661 aaactccggc cgcctggtgc cacacgcacc gggaatgcta cagcaaaacg accggtcgtc
      721 gctgcggcag ccctgacagt ctcagcaggc tgattctgag cagcattcag cccatagaat
      781 gagatgaccc gtatgaatga tggcagacag acgcctccgt cccctgaagc atggaaaatc
      841 cggtttctgg cgaacggtac
//
LOCUS       DAZGBC010000529          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGBC010000529 DAZGBC010000000
VERSION     DAZGBC010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.533"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..689
                     /locus_tag="ACHK1J_005526"
     CDS             <1..689
                     /locus_tag="ACHK1J_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HFO7914623.1"
                     /translation="MINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTF
                     SLTPEGRLTARNADISGAVTANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAF
                     PRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKC
                     NGRVLFERTALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVM
                     KKSTAGISIS"
BASE COUNT          202 a          203 c          261 g          183 t
ORIGIN      
        1 cgatgattaa cccggagaac ggcaacacca cgccgctgtt tgtggcgcag gggaatcagc
       61 tgttcatgaa cgatgtgttc ctgaagcgac tgtttgcagt gagcatcacc tcgtccggca
      121 atcccccgac gttctccctg acgccggagg gcaggctgac ggcccgcaat gcggacatca
      181 gtggagctgt cacagcgaat agcggcacgc tcaataatgt gaccattaac cagaactgcc
      241 ggatacttgg aaaactgtct gccaaccaga ttgaaggcga tattgttaaa accgtgggga
      301 aggctttccc gcgggactcc cgcgcaccgg agcggtggcc atcggggacc attaccgtca
      361 ggatttatga cgatcagcct tttgaccggc aaatagtgat cccggcagtg gctttcagcg
      421 gcgctaaaca tgagagagag cataacgata tttattcatc atgccggctg atggtgaaat
      481 gtaacggcag ggtgcttttt gaaaggacgg cgctggctga gtctcaggtg tattcagggg
      541 tgattgatat gcctgcagga agggggcata tgacgctgga gttttctgtg tctgcctggc
      601 tggtgaatgg atggtacccg acagcgagta tcagcgatct gctggttgtc gtgatgaaga
      661 agtccacggc gggtatcagt atttcgtgat catgaggagc cggattgccg gaaggagaca
      721 aaaaccgtac agtatgcgcg ggtgcctttg gctgatggcc ggagggaaca cctgaaggcc
      781 ggatgtgaaa aggccccgga caaacattca tgtttaaccc gaggcctgac cattcatcct
      841 aagcaagtg
//
LOCUS       DAZGBC010000530          839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGBC010000530 DAZGBC010000000
VERSION     DAZGBC010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.534"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(63..626)
                     /gene="ipbA"
                     /locus_tag="ACHK1J_005527"
                     /gene_synonym="fimX"
     CDS             complement(63..626)
                     /gene="ipbA"
                     /locus_tag="ACHK1J_005527"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO7914624.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          259 a          165 c          164 g          251 t
ORIGIN      
        1 ttttttatgg aagaattgag gaaaacgtag gaatctgaca ttttgaatca gaaggtactg
       61 ttttaacgag gctttttttt ccacacccct ttaaatcgag cagcattact ggctgtgtat
      121 ctaacagtat gctggatatt tcgatgaccc agataatcct gaagtagtcg ggtatcgaca
      181 ccattgtcag ccagagcaaa accacaggca tgacgtaaca tatgaggatg tgcttttaca
      241 gctaatcctg cacgttttcc agcctcacga atgatagaaa aaaaatgttg tctgctgata
      301 gggcggcgtt tccgggagag aaaaagccag tctccttcaa ctccgttttc aattaacttc
      361 ctctgcttaa gccacaactt gatcaaatta tattcatcag gaagaagagg atgtgtcgtc
      421 gagaaaccat ttttaatacg ccgaatatta agttgttttc ctgatgcatc aatatcggat
      481 aaacgtaaat caagaagttc actggcccta aaaccgtgaa tgaaagccat cataattaga
      541 cagcgatttc tttcaggaaa ggacatgctc tgggctgcat ccatcagcct gtagacttca
      601 tcctgtgtga gatattttct cgtcatacgc caccgtgtac agttgtaaaa aagagaggca
      661 cacccagata agtatgcctg ccatcagaac gttatcgatg taatctcatt atctcattaa
      721 taagattttt tgaatagtag ttctacgcaa tagactaaat atgaattatt tattgtgcag
      781 cttttctaca agccattttt aatgcgtgtt tttatctaca gcgatgtgga aaaaataaa
//
LOCUS       DAZGBC010000531          837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGBC010000531 DAZGBC010000000
VERSION     DAZGBC010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.535"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..147
                     /gene="tnpB"
                     /locus_tag="ACHK1J_005528"
     CDS             <1..147
                     /gene="tnpB"
                     /locus_tag="ACHK1J_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7914625.1"
                     /translation="KRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDWRQPKTSRRNS
                     LTML"
     gene            178..>837
                     /locus_tag="ACHK1J_005529"
     CDS             178..>837
                     /locus_tag="ACHK1J_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO7914626.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPAHLPREIHRLESEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     AR"
BASE COUNT          226 a          219 c          233 g          159 t
ORIGIN      
        1 aaacgcctgg aggaaggcca gtttatctgg cctgcggtac gtgacggcaa ggtatccatt
       61 acccgctcgc aactggcaat gctcctcgat aagctggact ggcgtcagcc aaaaacatcc
      121 cgccgtaact cactgacaat gttgtaaaaa aatcctgacc gcattataaa aacggtcatg
      181 agtcagaaat acctcattcg cattgcagaa ctggaaagcc agctccgtca gaaagaccag
      241 caactgagtc tggttgaaga gacggaggcc ttcctgcgct ctgcactggc acgtgccgaa
      301 gaaaagatcg aagaagatga acgggaaata gaacatctgc gggctcagat agaaaaactg
      361 cgccggatgc tgttcggtac ccgttctgaa aaactgcgtc gtgaagttga acaggctgag
      421 gccctgctga aacaacgcga acaggacagc gatcgttaca gtgggcggga agacgatcca
      481 caggttcccc gccagttgcg acagtcgcgc catcgtcgtc cgttaccggc acaccttccc
      541 cgtgaaatac accgtctgga gtctgaagaa agctgttgcc cggagtgtgg cggtgagctg
      601 gattatctgg gggaagtcag tgcagaacaa ctggaactgg tgagcagcgc cctgaaagtg
      661 atccgcacag aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg
      721 gcgccgtccc gcccgataga gcgtggtatc gcgggccccg gattacttgc ccgcgtgtta
      781 acgggaaaat actgcgaaca tctgccactg tatcgtcaga gtgaaatctt tgcccgc
//
LOCUS       DAZGBC010000532          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZGBC010000532 DAZGBC010000000
VERSION     DAZGBC010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.536"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..436)
                     /locus_tag="ACHK1J_005530"
     CDS             complement(<1..436)
                     /locus_tag="ACHK1J_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914627.1"
                     /translation="MSSSVKYSPVPAVLTRPESLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFCQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQRVPGDSVNCMCWASLAALAFDAEERVE"
     gene            complement(421..>836)
                     /locus_tag="ACHK1J_005531"
     CDS             complement(421..>836)
                     /locus_tag="ACHK1J_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914628.1"
                     /translation="PEGLPLLESGGGNGGVFYDNEPLLLIASRRLRASLETALIVLQK
                     WTVCVGCGYSGVRDGAGKNAPVWVKVNPESGLGLCSGCDSFLLQERQRRQRHDQLFSC
                     GLSCKQRKTEGAVYLRGSDAPGGLRDMEVKVCLPQ"
BASE COUNT          211 a          279 c          199 g          147 t
ORIGIN      
        1 gttccacccg ctcttcagca tcaaatgcca gtgcagcaag gcttgcccag cacatgcagt
       61 tcactgaatc ccccggaacg cgctgcagct cccgacgcac catgttgacc tccgcactga
      121 aaaaagcgtt ctcatcgcct tcaagggcag ccagatgcag cggaagccag acctgacaga
      181 accattcaca catccgttcc cggttactga cggtcatcac tgtctccggg cgggaaccgt
      241 cctgaaaaac atgtccggtg cgtggagaac tgtatgaaac ataaccaccg tgcaccgcaa
      301 tgaccggacg acctgacgcc ggaagcccca cacgacaccc actggcaatc cgggtcatca
      361 gcaggttcag ttcagccagc gattcagggc gggttaagac cgccggaacc ggagaatatt
      421 tcactgagga agacatactt tcacctccat atctctcagc ccgccaggtg cgtcacttcc
      481 ccgcagatat accgccccct ccgtctttcg ctgtttgcag gaaagaccac atgaaaaaag
      541 ctggtcatgc cgctgacgac gctggcgttc ctgcaaaaga aaggagtcac aaccggaaca
      601 cagccccaga ccactctcag gattcacctt cacccagacc ggagcattct taccggcacc
      661 atcccgtacc ccggaataac cacagcccac gcacaccgtc cacttctgca gtacgatgag
      721 tgcagtttcg agtgatgcgc ggagacgccg ggaggcaata aggagcaacg gctcattgtc
      781 ataaaaaacc ccgccgttac cccctccgga ttcaagcagc gggagtcctt caggct
//
LOCUS       DAZGBC010000533          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGBC010000533 DAZGBC010000000
VERSION     DAZGBC010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.537"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>836
                     /locus_tag="ACHK1J_005532"
     CDS             <1..>836
                     /locus_tag="ACHK1J_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914629.1"
                     /translation="SEAENQWVVQDTLKAYSEMDWLTAKLTSDETSPQIAGYMPEGAR
                     VEWNPADSNLNATGSNKRKYGLVTLKGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAG
                     PGWWRHLLPPAKEAMVFDKTVSLSSPFAIKVGDPIGHMGYYQAPKDGGYEARYQVHIE
                     CTSMDDNLEKFLTNPEQVGKNNPLWLKYAPGLALYKKDAFTGTFTKDARVTTRTGILP
                     LKQVQTEVDKSTKQEYWQLRPENAYVPKGQVEPQLLSQYDLAKLGFRTEIAEPTSFDY
                     LDG"
BASE COUNT          250 a          188 c          230 g          168 t
ORIGIN      
        1 tctgaagcag aaaatcagtg ggtcgttcag gacactctaa aagcatattc tgaaatggac
       61 tggctgacgg cgaaactgac ttcagatgaa acttctccac aaattgcagg gtacatgcct
      121 gaaggggcca gagtggagtg gaacccggca gacagcaatc ttaacgctac aggaagtaac
      181 aagagaaaat acggtcttgt gacgctaaag ggtttaccag aaaaaaacag cagtactctc
      241 actcccggtc agcgctactg ggttgttgtg gataacaaca atgttaagcc agcgccgggg
      301 gccgggcccg gctggtggcg acacttgctg cctcccgcaa aggaagccat ggtattcgat
      361 aaaacagtaa gcctcagttc tccatttgcc attaaggtcg gtgacccgat tgggcacatg
      421 ggctactatc aggccccaaa ggatgggggg tatgaggcac gatatcaggt acatatcgaa
      481 tgcacgagta tggatgataa tctggagaaa ttcctgacca atcctgagca ggtgggaaaa
      541 aataatccac tctggctgaa gtacgccccg gggcttgcgt tgtacaagaa agatgctttt
      601 acaggaacat tcacgaagga cgcgagagtg acgaccagga cgggaattct gccactgaaa
      661 caggtgcaaa cagaagtcga taagtcgacc aaacaggaat actggcaatt gcggccagaa
      721 aacgcgtacg tgccaaaagg tcaggtggaa cctcaacttt tgtcacagta tgatctggca
      781 aagctggggt tcagaactga aatcgcagag cctaccagct ttgattacct ggatgg
//
LOCUS       DAZGBC010000534          835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGBC010000534 DAZGBC010000000
VERSION     DAZGBC010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.538"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(33..524)
                     /gene="hcp"
                     /locus_tag="ACHK1J_005533"
     CDS             complement(33..524)
                     /gene="hcp"
                     /locus_tag="ACHK1J_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO7914630.1"
                     /translation="MAIPVYLWLKDDGGADIKGSVDVQDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NMKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            complement(530..>835)
                     /locus_tag="ACHK1J_005534"
     CDS             complement(530..>835)
                     /locus_tag="ACHK1J_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7914631.1"
                     /translation="GWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDWMRDTGDIPQSC
                     FAVQGYGESRPVAPNDSAEGRARNRRVEISLVPQADACRLPDASPAPEEGASENITE"
BASE COUNT          176 a          247 c          213 g          199 t
ORIGIN      
        1 gacggacagg ataccctgcc cgtccgggag aattacgcag tggtacgttc attccacgcg
       61 tcagaatgaa tgatgttacc gtccttataa gtccaggtga ttttctcgta gcgcagctcc
      121 acctgctcaa ggtggttatg cttctcgtag gcgggattct tgatgtcgtg catgaccggg
      181 ttaaccttca ccaccttcac gttctcaagc ttcatattga aatactccac ttcctgaccg
      241 gcatcattga ttctgtacca cttaaattca gcagacttca gggtctgccc cgtggtcacc
      301 gccttgtaaa ggtaggggct ggaggaatca atttccttgg taaacaggaa cggcgtgtga
      361 atacgggtac cggtcagctt accggtattg ttatccgtcg gaatgtaaag gcagtgctcc
      421 tgagccacca cctcaatgct gccctcacgg tcctgaacat ccacggaccc tttgatatct
      481 gcaccgccat catctttcag ccacagataa actggaattg ccataaaaat cactccgtta
      541 tgttttctga tgcgccctct tccggcgcag gggaagcgtc cggcagacga caggcatcgg
      601 cctgcggtac cagactgatt tcaacacggc ggttacgcgc gcggccttcc gccgagtcat
      661 tcggggccac agggcggctt tcaccgtatc cctgcacggc aaaacagctt tgagggatat
      721 cgcccgtatc ccgcatccag tcgcgaaccg cctctgcacg cttcagggac agggcctggt
      781 tgcgctgcgg gtcaccggta ttgtccgtgt ggccactgac cacaatcagc cagcc
//
LOCUS       DAZGBC010000535          833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZGBC010000535 DAZGBC010000000
VERSION     DAZGBC010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.539"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..>833
                     /locus_tag="ACHK1J_005535"
     CDS             135..>833
                     /locus_tag="ACHK1J_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFO7914632.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHR"
BASE COUNT          285 a          185 c          132 g          231 t
ORIGIN      
        1 actgtcttgc ccactgtaga caaaatcaat acaattctgc atatcagtta tctataaaag
       61 ataacaatat cattataaat aattatcata tccacacact caaggcagta cctagaaaac
      121 ataaagataa aatcatgtct gaatcccgca gcaattttcc agcacgacat ggtactccat
      181 ggaatactga agagttacag cgtcttctcg tatccatagc ggagggcgtc tcgctaaaaa
      241 aacttactca aacacatcaa cgatcgtccg ggggaatacg tggagcaatc aaacgcctgc
      301 ttccttctga gtttcttgag ttcaacgata taaacactgt gaatgatctc agcaattact
      361 tcaacaagat tcctcgccaa gaacacaata gtatcatcaa taatttaata tcattgagca
      421 ctgcaaaaac aattgctcat gataaagttg aaatacaaaa gaatagtttt aaacaaaata
      481 ccccactccc cctctgccaa cccaatacag attttaaaga ttatatagcc gatgaacatg
      541 atgtcattat gctaattcat tctgctatcg aaagtttatc taataaacga catcaatata
      601 ttcttcttat gcgactcggc acagatgatt cacctcattc tctggcagat attgccgtca
      661 aattaggaat ttcccgtgaa agggtaagac aacttcagga acgagccctt ggcttactag
      721 ctgctaaatc tcgctatgaa gggacacctg gagcttgcct taaagccctt attaactcaa
      781 taggtaattc accatatgat atagcagtat ggatttatcg tattgttcat cgg
//
LOCUS       DAZGBC010000536          830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZGBC010000536 DAZGBC010000000
VERSION     DAZGBC010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.540"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..536)
                     /locus_tag="ACHK1J_005536"
     CDS             complement(<1..536)
                     /locus_tag="ACHK1J_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFO7914633.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVASATGFGPY
                     DGLRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPGEE
                     DDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEGDGY
                     VLIYGGDAQSLPDEIFLS"
     gene            complement(529..>830)
                     /locus_tag="ACHK1J_005537"
     CDS             complement(529..>830)
                     /locus_tag="ACHK1J_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914634.1"
                     /translation="NELKGSWISGPTLTSGSSWNNACEENGVTFSLSEDNFSIDPVLG
                     YTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKEEVRKESPGNG"
BASE COUNT          198 a          236 c          217 g          179 t
ORIGIN      
        1 tttgaaagga agatctcgtc cgggagtgac tgcgcgtctc cgccataaat cagcacgtag
       61 ccgtcgcctt cctgccagag gtcatggcaa aggcccgggg ctgctttccg gaccacatcc
      121 ggatccccca tcaccagcag gagtcgcacc ttacgctgcc agcgacgccc atagcgaaag
      181 cgcaaatgtt ctgaaacagc atcttcgcta aaaaccggct catcgtcgtc ttcttcaccg
      241 ggaatggtgt tcgtcaccgg agtgatactc tcagcctttt tctgccccca ctggaaatac
      301 agcccggatt taccggcgaa gtcgaaagcc ttctccgtca gccagccgag taaaaacagc
      361 gtggcaacca gtatggcgca gaatgtgagt atgcgcagcc cgtcatacgg accgaagccg
      421 gtggccgaag cgacgttttc cggaaaggca taaaaaagaa atgccacgat gaccgtcaga
      481 aaaaaggcga cagtcacaac agagccgatg agtgttttaa cttttacctt agccattccc
      541 gggtgattcc ttgcgaactt cttctttagt gatgacggca acccagacgt cgtctttgtc
      601 agctgtgggc tgggcggcga taacctgcgc acctcgttgt tcaaaggcag catcagcaag
      661 cgtgatggca agccatggag cagcatggcc ggtatagccc agcacgggat cgatactgaa
      721 attgtcttct gaaaggctga atgtcactcc gttctcttcg caggcattat tccagctgct
      781 gcccgacgtc aatgtgggac cggagatcca gcttccttta agctcattgg
//
LOCUS       DAZGBC010000537          827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZGBC010000537 DAZGBC010000000
VERSION     DAZGBC010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.541"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            211..>827
                     /locus_tag="ACHK1J_005538"
     CDS             211..>827
                     /locus_tag="ACHK1J_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914635.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTTHADINTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSG"
BASE COUNT          255 a          197 c          184 g          191 t
ORIGIN      
        1 catttccgta atctgatgca tcaggggtat gcccaccaca aataactctg tacagcaaca
       61 gaaaaatttc cctggtcata tgatgaatta atcatcgcca tcacaccgga tgcttctcat
      121 cctgtcctca gaaaggcagg tggtctcctg tctgccgcgc ttatccagga taccgggacc
      181 tgaaaggtca taatctggca ggaaaaaata atgtccgaag taaaaaaaca cgtttcttat
      241 tcattaacag ttgcagctat gctgtcagcc gccatcgtct gtcagacaac acatgctgat
      301 atcaacacac cgaacatcac cggagaaaaa aacaatacaa caacacgatt ctccggaaat
      361 atttacggca gtgaaaatac tgtcacatca gagtcaaaca gtctggttgt gggtaacagt
      421 aattccgtgg agggagggta taacaacatt gttattggta attcctccac ggtcaaagct
      481 tccggtttga tcagaaaagt gggcgaagat attgtgctgg atggttacag cgtcagcctc
      541 ggtaatgcca ccagaattaa cggtgattca tctgttgcca taggtctgac atcgtccgta
      601 accgggaacg actccgtggc actgggcagt cattccagtg ctaacggaaa caatatcgta
      661 tccgtcggct ctgacacact gaaacgccgc atcacaaatc tggcacaggg gaaagatgac
      721 catgatgcgg taaatctgat gcagatgacc agtgctgtta accgggaagc tggagaaaga
      781 ataaaatcaa caaacaacct gcagaatcag cttaataccc tctctgg
//
LOCUS       DAZGBC010000538          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZGBC010000538 DAZGBC010000000
VERSION     DAZGBC010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.542"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..710
                     /locus_tag="ACHK1J_005539"
     CDS             114..710
                     /locus_tag="ACHK1J_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO7914636.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            713..>822
                     /locus_tag="ACHK1J_005540"
     CDS             713..>822
                     /locus_tag="ACHK1J_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914637.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEF"
BASE COUNT          322 a          143 c          139 g          218 t
ORIGIN      
        1 ataaagaatg gaataaatat gacttattaa ttttaaaatc tgttaatgaa atcaacattc
       61 atttgtccag cactccatac tttcagccgt tggattggta tataataaaa gccatgctct
      121 ggactgaaaa cgatgcggaa aatacttctc agtggaatgg ttatccatta caaattggtc
      181 gttttcggaa agataaagcg atgccagctt taatctctgg agaaaaatct acagcccttg
      241 ttaccccacc acaatggaga aacaaagcat tcaatgggtt aaaagatcca gaaagaaatt
      301 actgggcaaa agaacaaata actggctctc cagaagagaa tatcaaagca gcaattactt
      361 acttaatgat gaaactttct aatacaaagg aagaatcaac tatcgatcaa tatgactcaa
      421 ctctatatag cgccattgtc caaaaaggcg atttggctga caacatcaga aaagagagaa
      481 aaacgaccat acctaactta acgaaaaaca atcctggaaa aaatctcgat aaaatacacc
      541 cgggagatat tttatattat caaaaagcat ccatgaaagt aataattact ggatggaaac
      601 ctatcacaat aaaaaatgta gcgatgaatt ataatggtgg tggcgatccc aagtatgcaa
      661 taaaacttca gttcgtttat acattactca caaaaaacag agtattatga gaatgcgaaa
      721 aataacaatt attctactgc tgacattctc atgtacaaaa ggtctggcat ttgacatttc
      781 catgtctaaa gaatgtagcg gtgttgatga acttgaattt at
//
LOCUS       DAZGBC010000539          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZGBC010000539 DAZGBC010000000
VERSION     DAZGBC010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.543"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..572
                     /locus_tag="ACHK1J_005541"
     CDS             <1..572
                     /locus_tag="ACHK1J_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFO7914638.1"
                     /translation="AFQHYCQSLEQIEDRILQLDQEISRLLPEWSLCNLVCQLQALKG
                     VGQLIAITLVAELGDFSRFSNPKQLMAFLGLVPGEYSSGNSIRPRGITKVGNSELRRL
                     LYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVA
                     ITAVARELTGFMWDIALAAQSSFSQQKQN"
BASE COUNT          239 a          178 c          190 g          215 t
ORIGIN      
        1 ttgcttttca acactattgt caaagtctgg agcagattga agatcgcata cttcaactcg
       61 accaggaaat atctcgcctg ttacctgagt ggtctctgtg caatctggtc tgccagttgc
      121 aggctctaaa aggtgttggt cagctaattg caataaccct ggttgctgaa ctgggtgatt
      181 tttcgcgatt ttccaatccc aaacagctca tggcttttct gggactggtg ccaggtgaat
      241 attccagtgg aaacagtatt cgtcccagag gaataacaaa agttggaaac agcgaactga
      301 gacgtctgct ttacgaagcc gcctggtctt atcgtacacc tgcaaaagtt ggtgcatggc
      361 ttatatatta ccgaccggac tctgtaacac aatattccaa agatattgca tggaaagctc
      421 aacaacgatt gtgttctcgt taccgaactc tgacagcaaa agggaaaaaa tcacaagtag
      481 ccattacggc ggtggctcgt gagttgactg gatttatgtg ggatattgca cttgctgccc
      541 aatcatcatt cagtcagcag aagcaaaatt aaaccctgaa gcagtcagac acggaatgag
      601 tgatcctcga aaaagaattc agggaagcca gaatgcgcta atacctgcac gtagaaagag
      661 gccagctcac tgcgtaaatc gtcatggggc taaaatcccc gaataagagt ttgtaaaatc
      721 caagatacag tctgactgtt tcaggttctt taatccgcga tataacgaag ctcccggagc
      781 tgcgtaggtc tttttgggct taatatttga caaaggtcat aa
//
LOCUS       DAZGBC010000540          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZGBC010000540 DAZGBC010000000
VERSION     DAZGBC010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.544"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..>821
                     /locus_tag="ACHK1J_005542"
     CDS             52..>821
                     /locus_tag="ACHK1J_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7914639.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAG"
BASE COUNT          268 a          160 c          162 g          231 t
ORIGIN      
        1 cttagcaaac tatgtgattt ccatttcgat tgatttagtg tttattgacg tatgtactgg
       61 attattaacg ataatatcga gttctggcct gagcaccgaa aattaatatc ggtacataac
      121 gccgatctta acgtcgttct gacaacgcca gccagtcgtt gtttatccct tctacttgaa
      181 gcttttcctg atgtggttgc acaacaagat tttttcacca gagtctggga agaagaaggt
      241 atgcgtgtgc ctactaacac gttatatcag aacatatcca ttatcagacg cggatttcgc
      301 gctgttggtg atactaccca ctcgcttatt gcaaccgtgc cgagaagagg attcaagatc
      361 cataatgaca ttaacataca aaatcatgta ataaactcgt caacagacgc acatacacac
      421 aatgccccac cggccataaa agttaatgcg ggatacaaag agagcattgg tggcgcaaag
      481 aatttcaata acaaaatcct caaacatata aaaagttatc taattatgtt gagcgcattt
      541 gtcataggcg catattctgc atattggcta tggaataata atcaaccgaa gccatttttc
      601 aaggattaca agactgttgc ggaaattaat ggctgccatt ttaatgtaac agaagataca
      661 attgatgggt tgaaagagtt cgataaatat aaaacacgga tactggattc ggggattaac
      721 tgtaaaaaat atccgtggtt atacttccct cttgcaaaat cctcaccggg gatgattgtt
      781 atggcatgca ataaaaacta taaccaacat gaagtggcag g
//
LOCUS       DAZGBC010000541          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZGBC010000541 DAZGBC010000000
VERSION     DAZGBC010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.545"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>821
                     /locus_tag="ACHK1J_005543"
     CDS             <1..>821
                     /locus_tag="ACHK1J_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7914640.1"
                     /translation="FTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLV
                     QMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLP
                     LALISAEGATEWCAEYDEWGNLLNEENPQQLQQLIRLPGQQYDEESGLYYNRHRYYDP
                     LQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNEES
                     INENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFY"
BASE COUNT          224 a          185 c          240 g          172 t
ORIGIN      
        1 cttcacgcca ctcatcaggg ttgaaaccgc caccggtgag ctggcgaaaa cgcagcgccg
       61 cagcctggcg gatgcgcttc agcagtccgg cggcgaagac ggtggcagtg tggtgttccc
      121 gccggtgctg gtgcagatgc tcgaccggct ggaaagtgaa atcctggctg accgggtgag
      181 tgaggaaagt cgccgctggc tggcatcgtg cggcctgacg gtggagcaga tgcaaaacca
      241 gatggacccg gtgtacacgc cggcgcgaaa aatccacctg taccactgcg accatcgcgg
      301 cctgccgctg gcgcttatca gtgcggaagg ggctacagaa tggtgcgcag aatacgatga
      361 atggggcaac ctgctgaatg aagagaaccc gcaacagctg cagcagctca tccgcctgcc
      421 gggtcagcag tatgatgagg agtccggcct gtattacaac cgccaccgct attatgaccc
      481 gctgcagggg aggtatatca ctcaggatcc gattgggctg aaggggggat ggaacctgta
      541 tacatatccg ctgagcccgg tgaatagcat ggatccatta ggattatatg aatttaaatc
      601 aaaaaatata gatgatattg gaatatttgc attggcaatg tgtaatgaag aatcaattaa
      661 cgagaataaa gaatatggtg gactaatatg taagaagcaa ggtgaatatt tccccatgaa
      721 tccgataagt tcaaatgata atgatagtgt agacttgcga aatataaaat gccctgaagg
      781 ttcagagaga gtaggtgatt atcacactca cggtttttac t
//
LOCUS       DAZGBC010000542          820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZGBC010000542 DAZGBC010000000
VERSION     DAZGBC010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.546"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..767)
                     /locus_tag="ACHK1J_005544"
     CDS             complement(<1..767)
                     /locus_tag="ACHK1J_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7914641.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMQEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQSPRQVGQLSVMYRCLPDCHHDEAVLRFTLKTLRQQ
                     CKQIKSLTGFTLPVVLSAEFSGPETPWIIVRGDKPVVCPVNHAPQAFTDWLQVEDNIL
                     ALPVISEAFSFIRNTLADELEKADRLMPPVRAFSVVMR"
BASE COUNT          215 a          228 c          211 g          166 t
ORIGIN      
        1 cgcattacaa cggaaaatgc ccgtacaggt ggcatcagcc ggtcagcttt ttccagctca
       61 tcagccagcg tattacggat aaaggaaaat gcctcgctga tgaccggcag tgccagaata
      121 ttgtcttcca cctgcagcca gtcagtgaat gcctgaggtg catgattaac cggacataca
      181 actggcttat ccccccggac gattatccac ggtgtttcag gcccggaaaa ttcggcactc
      241 aacaccaccg gcagtgtgaa acccgtcagg gattttatct gcttacactg ctggcggagt
      301 gttttcagtg taaaacgaag aaccgcttca tcatgatgac agtctggcag gcagcggtac
      361 atcacagaga gctgaccgac ctggcgcggg gactgcgtct gtatgctgcg gacaacgtca
      421 gttaagcgac tgacgtcacc gacccgcagc caccatcccc gggctgtttt acgcagcgga
      481 ccgtcctgaa acaacgcttc ctgcatgtct ccacatacca gtacaacagg gccccgggtg
      541 tcggataatg ccggttcagc atccgtcagg gtaacgtcag atgtgagctt ttgcttttca
      601 ctccggctgt ttttaaaaac actccagcct gccagaagaa ggatcagcag agtagccaga
      661 ccgttaaaaa cgttgcctgc agggatgaaa taccacagca accacagcaa cgtggaaacg
      721 gccaccaggg catgcagggc taccggaatg atgacagatc cacgcatcag ccatgcaact
      781 ccggtatttg tgatgacaaa agagactgaa gccagaggta
//
LOCUS       DAZGBC010000543          820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZGBC010000543 DAZGBC010000000
VERSION     DAZGBC010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.547"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..726
                     /locus_tag="ACHK1J_005545"
     CDS             <1..726
                     /locus_tag="ACHK1J_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7914642.1"
                     /translation="ARTEGDATALAAARAHSDENDKRTLVSANTYADQKETAINNRTD
                     GLLTTERQARTAGDTATLAAARTHSDENNKRTLISANTYADQKETDINNRTDGLLMTE
                     RLARIAGDAETLATAEAYSDAGDSLTLKQATAYSDISTRRALSEANRYTDNKFRNLSN
                     KIERAEKRLNAGIAGVTAISSIPYVAENSFSYGIGVGNYQYANAVAAGIQYKTSRNTN
                     IRLNISLDSSHNTAIGAGVAGGW"
BASE COUNT          258 a          223 c          193 g          146 t
ORIGIN      
        1 gcccgaacag aaggtgatgc aacagcgctg gctgcagcca gagcccacag cgatgaaaac
       61 gacaaaagaa cactggtcag tgcgaatacc tatgctgacc agaaagaaac cgccatcaat
      121 aaccgtactg acggattact gacgaccgaa cgccaggcac gcaccgcggg cgatactgca
      181 actctggctg ctgccagaac gcacagcgat gaaaacaaca aaagaacact gatcagtgcg
      241 aatacctatg ctgaccagaa agaaaccgac atcaataacc gtactgacgg attactgatg
      301 accgaacgcc tggcacgcat cgcaggtgat gcagaaactc tggcaacagc agaggcatac
      361 agtgacgccg gggattcact gacactgaaa caggcaacag cctacagcga tatcagtacc
      421 cgccgggcac tgtcagaggc caatcgttat accgacaaca aattcagaaa cctgagcaat
      481 aaaattgaac gggcagaaaa aagacttaat gcaggcatcg ccggagtgac ggcaatatcc
      541 tccatcccct atgtggctga aaactctttt tcatacggga tcggcgtggg taactatcag
      601 tacgctaatg ctgtggccgc aggcattcag tataaaacat cacgtaatac caatatacgc
      661 ctcaacatct ccttggactc atctcataac accgcgattg gtgcaggggt tgccggcggc
      721 tggtaaataa tcttgcgtac atacggcatt atggtattcc gtaatcacat taattcagta
      781 cgcaacaccg ccccatccgg aacagaccac tggactgagc
//
LOCUS       DAZGBC010000544          816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZGBC010000544 DAZGBC010000000
VERSION     DAZGBC010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.548"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>816
                     /locus_tag="ACHK1J_005546"
     CDS             <1..>816
                     /locus_tag="ACHK1J_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO7914643.1"
                     /translation="GKLERQKFLFPLDYENKLQHQLDKHFTIESHPLLYRHTMGSKIA
                     NVIFEKLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINENIAKDAVTNNIALMSTFY
                     DSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQD
                     TVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDATN
                     LINLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENGSL"
BASE COUNT          281 a          119 c          150 g          266 t
ORIGIN      
        1 ggaaaacttg aaagacaaaa gtttcttttc ccactggact atgagaataa attacaacat
       61 caattagaca agcattttac gattgaatcg cacccattat tatacaggca tactatgggt
      121 agtaagattg cgaatgtgat ttttgaaaaa ttatacagtc gtattgactt taacaaagaa
      181 caatatatat cctttataaa ggacgcgttt cttcattttt atgattatag caggcgatat
      241 gcaattaatg agaatattgc aaaagacgct gtaacaaaca atatcgcatt gatgagcact
      301 ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac agaagaagag
      361 tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc tgatgataat
      421 tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc ctttcaagat
      481 accgtgttta atctgtttcc tgaaatgcac tcttcgcatt acaacgatat ttgtagcgta
      541 agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa tgaagaagtt
      601 ttcatgtcat tgcctgttcc tgttgccacg atggtacgtg aagatgcaac taatttaatc
      661 aatcttaaaa cactactcaa tgatggttgc tttattaaat atagtcattt taatgacgtg
      721 gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt gaatgaaagc
      781 atattgaaaa aatgttgttt tgaaaacggc agttta
//
LOCUS       DAZGBC010000545          810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZGBC010000545 DAZGBC010000000
VERSION     DAZGBC010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.549"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..757)
                     /locus_tag="ACHK1J_005547"
     CDS             complement(<1..757)
                     /locus_tag="ACHK1J_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7914644.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVLRFTLKTLRQQ
                     CKQIKSLTGFTLPVVLSAEFSGPETPWIIVRGDKPVVCPVNHAPQAFTDWLQVEDNIL
                     ALPVISEAFSFIRNTLADELEKADRLMPPVRAFS"
BASE COUNT          213 a          226 c          208 g          163 t
ORIGIN      
        1 cggaaaatgc ccgtacaggt ggcatcagcc ggtcagcttt ttccagctca tcagccagcg
       61 tattacggat aaaggaaaat gcctcgctga tgaccggcag tgccagaata ttgtcttcca
      121 cctgcagcca gtcagtgaat gcctgaggtg catgattaac cggacataca actggcttat
      181 ccccccggac aattatccac ggtgtttcag gcccggaaaa ttcggcactc aacaccaccg
      241 gcagtgtgaa acccgtcagg gattttatct gcttacactg ctggcgaagt gttttcagtg
      301 taaaacgaag aaccgcttca tcatgatgac agtctggcag gcagcggtac atcacagaga
      361 gctgaccgac ctggcgcggg aactgcgtct gtatgctgcg gacaacgtca gttaagcgac
      421 tgacgtcacc gacccgcagc caccatcccc gggctgtttt acgcagcgga ccgtcctgaa
      481 acaacgcttc cggcatgtct ccacatacca gtacaacagg gccccgggtg tcggataatg
      541 ccggttcagc atccgtcagg gtaacgtcag atgtgagctt ttgcttttca ctccggctgt
      601 ttttaaaaac actccagcct gccagaagaa ggatcagcag agtagccaga ccgttaaaaa
      661 cgttgcctgc cgggatgaaa taccacagca accacagcaa cgtggaaacg gccaccaggg
      721 catgcagggc taccggaatg atgacagatc cacgcatcag ccatgcaact ccggtatttg
      781 tgatgacaaa agagactgaa gccagaggta
//
LOCUS       DAZGBC010000546          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZGBC010000546 DAZGBC010000000
VERSION     DAZGBC010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.550"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>806
                     /locus_tag="ACHK1J_005548"
     CDS             <1..>806
                     /locus_tag="ACHK1J_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO7914645.1"
                     /translation="ARKIHLYHCDHRGLPLALISTEGATEWCAEYDEWGNLLNEENPH
                     LLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISD
                     IDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKE
                     FAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEI
                     FSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNGKGLPNVCRV"
BASE COUNT          254 a          162 c          200 g          190 t
ORIGIN      
        1 ggcgcgaaaa atccacctgt accactgcga ccatcgcggc ctgccgctgg cgcttatcag
       61 cacggaaggg gctacagaat ggtgcgcaga atacgatgag tggggcaacc tgctgaatga
      121 agagaacccg catctgctgc agcagcttat ccgcctgccg gggcagcagt atgatgagga
      181 gtccggcctg tattacaacc gccaccgcta ttatgacccg ctgcagggga ggtatatcac
      241 tcaggatccg attgggctga aggggggatg gaacctgtat ggatatcaat tgaatccgat
      301 atcagacatc gaccccctgg gtttatctat gtgggaggat gcaaaatcgg gggcatgtac
      361 taatggtctt tgcggcacac tatccgctat gataggtcca gataaatttg attctataga
      421 tagcaccgca tatgacgcct taaataaaat aaatagccaa tctatttgcg aagataaaga
      481 gttcgctggt ttaatatgta aggataatag tggcagatat ttctcaacag cacctaaccg
      541 aggagaaaga aaaggatcat atccattcaa tagcccttgc cctaatggta ctgagaaagt
      601 atcagcttat catactcatg gtgcagatag tcatggagaa tattgggacg aaatattttc
      661 aggtaaagat gagaaaatag ttaaaagtaa agataacaat atcaagtcat tttatttagg
      721 tacgcccagt ggtaatttta aagcaataga taaccacggg aaggaaataa caaacggaaa
      781 aggattacct aatgtctgca gagttc
//
LOCUS       DAZGBC010000547          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZGBC010000547 DAZGBC010000000
VERSION     DAZGBC010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.551"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(13..>803)
                     /locus_tag="ACHK1J_005549"
     CDS             complement(13..>803)
                     /locus_tag="ACHK1J_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7914646.1"
                     /translation="TEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRH
                     RYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDPLGLDLLSLPSPRQKPVSQSCT
                     IDPINQQPVGRFIGDSKGNIMVEPVGGRTIPYPPSNPGSADTHTLYQNNSNAYRLNPI
                     GHANNSTPHAHAHLPGTGSGMKGQGPSLDVNGRVVPWNSSAAHIGIRSLGTLSFFSSV
                     LHMFTLRSCREGTFGQCYCAMYVEMSTGSNQDEADIMCGMPEYF"
BASE COUNT          218 a          191 c          162 g          232 t
ORIGIN      
        1 taccccccta ttctaaaaat actcaggcat tccgcacatt atatcagcct catcttgatt
       61 gcttcccgtt gacatttcaa catacatagc acaataacat tgtccaaacg ttccctctct
      121 acatgatcta agtgtaaaca tatgtaaaac agaactaaaa aagcttagtg taccgagtga
      181 tctaataccg atatgtgcgg cactactatt ccatggtaca acgcgaccat taacatcaag
      241 ggatggacct tggcctttca taccgctacc tgttcctggt aaatgtgcat gtgcatgtgg
      301 tgttgaattg tttgcatgcc ctattggatt aagcctataa gcatttgaat tattctgata
      361 aagagtgtga gtatctgcac ttcctggatt actgggggga tacgggattg ttctgccacc
      421 taccggttca accataatat tgcctttaga atcacctata aacctaccaa caggttgttg
      481 atttatagga tcaatagtac aggattgact tacaggtttt tgtctgggag aaggtaatga
      541 taatagatct aatcccaaag ggtctatata ctgaaccgga ttcaacggat actgataaaa
      601 attccatccc cccttcagtc caatcggatc ctgagtgata tacctcccct gcagcgggtc
      661 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
      721 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
      781 gtattctgcg caccattctg tag
//
LOCUS       DAZGBC010000548          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZGBC010000548 DAZGBC010000000
VERSION     DAZGBC010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.552"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..404
                     /locus_tag="ACHK1J_005550"
     CDS             <1..404
                     /locus_tag="ACHK1J_005550"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7914647.1"
                     /translation="VGTKVSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISK
                     LALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSI
                     SCEVTFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            401..748
                     /gene="tnpB"
                     /locus_tag="ACHK1J_005551"
     CDS             401..748
                     /gene="tnpB"
                     /locus_tag="ACHK1J_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7914648.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          191 a          221 c          215 g          176 t
ORIGIN      
        1 tagtgggaac caaagtgtca gatatgcaga aaaatgtgac tcccggcagg cgaaaaggct
       61 gccctaatta tcctcccgaa tttaaacagc agctcgttgc tgcctcctgt gaacccggga
      121 tatccatctc aaaacttgct cttgaaaatg gcattaacgc caatctgttg ttcaaatggc
      181 gacaacaatg gcgcgaggga aagctgctat taccttcttc agagagcccc cagctacttc
      241 ctgtgactct cgatgcagct gccgaacagc cagaatcgct cgcagaggac ccggaaaccc
      301 tcagtatcag ctgtgaggta acgttccggc acgggacgct ccgcttcaat ggcaatgtca
      361 gcgaaaagct cctgactctg ctgatacagg aactgaagcg atgatcccgt taccttccgg
      421 gaccaaaatt tggctggttg ccggtatcac cgatatgaga aatggcttca acggcctggc
      481 tgcgaaagta cagacggcgc tgaaagacga tcccatgtcc ggccatgttt tcattttccg
      541 gggccgcagc ggcagtcagg ttaaactgct gtggtccacc ggtgacgggc tgtgcctcct
      601 gaccaaacgg ctggagcgtg ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt
      661 ccttacgcag gcgcagctgg cgatgctgct ggaaggtatc gactggcgac agcctaagcg
      721 gctgctgacc tccctgacca tgctgtaaat ctctttatcc tggttgtcac agaataagcc
      781 cggtaaaata cgggcttatg aac
//
LOCUS       DAZGBC010000549          801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZGBC010000549 DAZGBC010000000
VERSION     DAZGBC010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.553"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          287 a          122 c          154 g          238 t
ORIGIN      
        1 atatccccaa aatgataata atggattgaa actgtaggag cctgaaaaac tgatgtaaaa
       61 aaacaaactt acagcaaaaa aagaagcaat cctaatatcc ataaaggcat ctggaccact
      121 gattaaagga aataaatagg ttaagaagta attggttatt tccttgtccg ctggagaagc
      181 ggtagttaat ttcttactgg ttacggaatt atatttaatt gagtgtttaa caatcaagaa
      241 acacagtaaa agtataattg cgcacaatga taagtaagcg aacataaatt tatagtgctt
      301 tgatatacat gaaagaaaag ctaaggtgaa aaatactgga gcaacttagc tagcagtaag
      361 aagatatttg gcaagcttat ttaacatatt atcctcctta tctgcgctga tacctgtata
      421 taaaaacagt ataatatcaa tttaagaatt aaagaagata ataaaagtca attaaatcag
      481 catgatgtct gtgttttgaa tgtgtggggt ttttggggcg agtaatgtac gcgcgtattc
      541 tctagaggtt taatttaaaa ggctacgtat gtagctttat gtatttaaga gtactgaact
      601 cagtctccgg caataaacca gtttggcgtc cagctaacaa actcttgcgg atgaaaggtc
      661 cgggcacagc gatgacttac aagcattctc ccgcaaaaaa tgaactatag cgccttgctt
      721 aatgcattgt ctgaatccgg agttactttt taataattct gttaatggtt attagctcac
      781 ctaatatttc gaagttatca g
//
LOCUS       DAZGBC010000550          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZGBC010000550 DAZGBC010000000
VERSION     DAZGBC010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.554"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..414)
                     /locus_tag="ACHK1J_005552"
     CDS             complement(<1..414)
                     /locus_tag="ACHK1J_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914649.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLNGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MLAVVLFLGALFMEGVSSVILFRGCGSAAVSGEGRG"
     gene            complement(411..>800)
                     /locus_tag="ACHK1J_005553"
     CDS             complement(411..>800)
                     /locus_tag="ACHK1J_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7914650.1"
                     /translation="DARRLKYLRKISPYVFEELLLLAFEQQGMPVIRNPSYSGDGGLD
                     GQVVINGERWLIQAKRYRRTIRPAHIDAFGELLRRKGCRGFFIHTGRTGNISRARLTL
                     WPDIELISGQRLLQLLNGTLLSNKGES"
BASE COUNT          214 a          253 c          166 g          167 t
ORIGIN      
        1 ccccctgcct tccccggata cggctgcaga accacaacca cgaaacagaa tgactgacga
       61 gacaccttcc ataaacaaag cgcccagaaa cagaaccacc gccagcattt tcagttcacg
      121 aatatcttca gcaacagcct ccccgaaaga aatcgctgtt cgcggacatt cctgctcatc
      181 gacatacatc cctgagacgg atatgacgga ggaacatttt tgtaccatca catgcccttc
      241 aggcgcatca cgccagcggg atgactccgt cgcataacgc tgtgtcatgc ctgcctcacc
      301 accattcaga ttgttccaga catacagcat taccagacat cctgccagca ttgacggcca
      361 gacaatcaga agccggagca gcagcataac aaaccggtac agtgtcataa tcatgattct
      421 cctttgttac tcagtaaggt gccattcagc agttgcaaca gacgttgtcc actgatcagc
      481 tcaatatccg gccacagtgt cagccgtgcc cggctgatgt tccccgtacg ccccgtatgg
      541 ataaaaaagc ccctgcatcc cttccggcgc agcagttcgc caaaagcatc aatatgtgcc
      601 ggacgaatgg ttcttctgta acgtttagcc tgtatcagcc agcgttcacc gttaatcacc
      661 acctgaccat caagaccacc atcgccgctg tatgaggggt tacggataac cggcatcccc
      721 tgttgctcaa aagccagcag caacagttcc tcaaacacat acggactgat tttgcgcaga
      781 tatttcagcc gccgggcatc
//
LOCUS       DAZGBC010000551          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZGBC010000551 DAZGBC010000000
VERSION     DAZGBC010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.555"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..414)
                     /locus_tag="ACHK1J_005554"
     CDS             complement(<1..414)
                     /locus_tag="ACHK1J_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914651.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCPSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRG"
     gene            complement(411..>800)
                     /locus_tag="ACHK1J_005555"
     CDS             complement(411..>800)
                     /locus_tag="ACHK1J_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7914652.1"
                     /translation="DARRLKYLRKISPYVFEELLLLAFEQQGMPVIRNPSYSGDGGLD
                     GQVVIGGECWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRTGNISRARLTL
                     WPDIELISGQRLLQLLNGTLLSNKGES"
BASE COUNT          211 a          254 c          167 g          168 t
ORIGIN      
        1 ccccctgcct tccccggata cggctgcaga accacaacca cgaaacagaa tgactgacga
       61 gacaccttcc ataaacaaag cgcccagcaa cagaaccact gcctgcattt tcagttcacg
      121 aatatcttca gcaacagcct ccccgaaaga aatcgctgtt cgcggacatt cctgctcatc
      181 gacatacata cctgagacgg atatgactga gggacatttt tgtaccatca catgcccttc
      241 aggcgcatca cgccagcggg atgactccgt cgcataacgc tgtgtcatgc ctgcctcacc
      301 accactcaga ttgttccaga catacagcat taccagacat cctgccaaca ttgacggcca
      361 gacaatcaga agccggagca gcagcataac aaaccggtac agtgtcataa tcatgattct
      421 cctttgttac tcagtaaggt gccattcagc agttgcaaca gacgttgtcc actgatcagc
      481 tcaatatccg gccacagtgt cagccgtgcc cggctgatgt tccccgtacg acccgtatgg
      541 ataaaaaatc ccctgcatcc cttccggcgc agcagttcgc caaacgcgtc aatgtgtgcg
      601 ggacgaatgg ttcttccgta acgtttggcc tgtatcagcc agcactcacc gccaatcacc
      661 acctgaccat caagaccacc atcgccgctg tatgaggggt tacggatcac aggcattccc
      721 tgttgttcaa aagccagcag caacagttcc tcaaacacat acggactgat tttgcgcaga
      781 tatttcagcc gccgggcatc
//
LOCUS       DAZGBC010000552          797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZGBC010000552 DAZGBC010000000
VERSION     DAZGBC010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.556"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            268..453
                     /locus_tag="ACHK1J_005556"
                     /pseudo
     CDS             268..453
                     /locus_tag="ACHK1J_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(473..>797)
                     /locus_tag="ACHK1J_005557"
     CDS             complement(473..>797)
                     /locus_tag="ACHK1J_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO7914653.1"
                     /translation="RAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPFMPFWQVTL
                     LMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFWFGLLMASFHWWRRKVNRLP
                     PWDDV"
BASE COUNT          206 a          215 c          203 g          173 t
ORIGIN      
        1 gaaattttcc cacgctaaga gaattgagtt gaacgagtgg ttacatccag gccagctaac
       61 tcactgaacg taaaggctgt acgaaatgtt ccggccgtcc gataaccgtt tggcttactt
      121 ccgtacattc ccttgcgtag atgtacagaa ttgtacgatt tcggacacca aaaccgctgt
      181 tgtcccgaca ttctggcgat tcctgaaagt cctttgacta aaaaatagtc ttaacgtggt
      241 ttattttccg aattccaagc ggacccctat caggcctgcc gtggggattt tgtggtgcgt
      301 ctcgacggca gcacctgtct gcagttatgg aataaagaag gcagggtggt acgccgggag
      361 ggcgatccgc tggaagtggc gcagtggctg ctggcctgtc atgacgcagg aatagaagtt
      421 cgcgtacaga taaatgaaag tgccgctccg taagaacacg cctgtgcacg gtttacacat
      481 catcccatgg tggcagccgg ttaactttcc tgcgccacca gtggaaagag gccatgagca
      541 gtccgaacca aaaacccccc gtgatactta taataatggc ctcacctgcc accattcctg
      601 acggccccca gtagataaac cacattgcac atccccagga aatgccccat aagctcccca
      661 tcaacagcgt gacctgccag aacggcataa agggtaatgg cggaagccgg atacccagcc
      721 gccagagaat acgcagaaga ggaggggcat aattgctccg ccacatcttt ttgctgtcca
      781 tcagggcaat ggcccgg
//
LOCUS       DAZGBC010000553          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZGBC010000553 DAZGBC010000000
VERSION     DAZGBC010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.557"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>795
                     /gene="pilO2"
                     /locus_tag="ACHK1J_005558"
     CDS             <1..>795
                     /gene="pilO2"
                     /locus_tag="ACHK1J_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO7914654.1"
                     /translation="YAITELTLPGETPRYSFVSVVDGVLVSDLVGSGEEVREARDTFL
                     SINTEPTQGWIHYEPAAFSAGDQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSL
                     IIALLGTAWYGWQYYERWQTEKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIW
                     TSLPLSAAGWRYSLAECSTDGGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMP
                     EGKTGGIGLPVTFRLSENAVTVDALPETAVLQERLTSLAQSMQLKLD"
BASE COUNT          172 a          238 c          222 g          163 t
ORIGIN      
        1 tatgccatca cggaactgac gttacccgga gaaacaccgc gctatagctt tgtttccgtt
       61 gtcgacggtg ttctcgtcag tgacctggtg ggaagcgggg aagaggttcg ggaagcccgg
      121 gatacctttc tttccatcaa caccgaaccg actcagggct ggatacacta tgaaccggcg
      181 gcattttcag caggcgatca gaatcagcac ctgccgctgg cctccctgac tggctctggt
      241 aaacatccgg ctgccgcacg cctgaatccg gtatcccggg gaccgcagtt catcactgtc
      301 tctctgatca tcgcccttct cggcactgca tggtacggct ggcagtatta tgaacgctgg
      361 cagacagaaa aagccgcgca ggcagcggtt gcccgacagg ccacagagga acgaatcact
      421 cccccctggc ccggactccc tgaaacagac gcgttcatcc ggggatgtgc tgatatatgg
      481 acttcactgc cactgtctgc tgccggatgg cgttattcgc tggcagaatg ctcgactgac
      541 ggtggcagcg gcaatcttcg cgcatcgtac accaatactg cgggcgcgac agtgactgac
      601 ttcatccggc gcatcagtga tatgacagac agccgaccgt ttattgtgat gccggaagga
      661 aagaccggcg gcattggcct gccggtcacc ttccggctgt ctgaaaacgc cgtcacagtt
      721 gatgcactac cggaaaccgc tgtcctgcag gaacgactga cctcgctggc tcagtcaatg
      781 caactgaaac tggac
//
LOCUS       DAZGBC010000554          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZGBC010000554 DAZGBC010000000
VERSION     DAZGBC010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.558"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..633)
                     /gene="fimE"
                     /locus_tag="ACHK1J_005559"
     CDS             complement(37..633)
                     /gene="fimE"
                     /locus_tag="ACHK1J_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO7914655.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          246 a          187 c          146 g          214 t
ORIGIN      
        1 ttatttattt tttaacttta ttatcaatga gttaaatcaa acctcttctc tttttaattt
       61 ttcgtttatg agattatttc tttcccataa tccggcaaaa cgagcagcat tactggcggt
      121 ataacgtaca gtatggcgaa tatttcgatg cccgagataa tcctgaatta aacgagtatc
      181 tgcaccacgc tccgccagtt cataaccgca agcatgcctt aacatatgag gatgagtctg
      241 cgttacggtt ccagcttcaa taccggcatc acgaataatg cgataggcct gctggcgaga
      301 aagccgactc ccgcggcgag aaataaatat ggcgtcggtc cggtcagcgc ctttccagtt
      361 agcacgttcc agggtccagc gttccacagc ttcacgctca tcaaaacgta acgggtgaac
      421 ggtagaaaat ccgttcttca gtcggcgaat atttattcga ccttcattaa ggtcaagatc
      481 ctgataatgc agatcaagca gttcactaat acgcatccca tgccgatatg ccaacagaat
      541 aagacaataa tctctggctc ccgttgcccc gtaacaaacc gcctgcatca tggcctgaac
      601 ttctttaccg gtaagataac gacgtttact cacaatagta gtactcctaa ctgagatata
      661 tttgaatgaa tacctatagg aaacctcaat cggtcaaaat tagcccaata gataaaaaga
      721 taaccacaca cgaagtgatg tggctatcag tcaattacat taaatatcat acaaaaataa
      781 aatatcactg atg
//
LOCUS       DAZGBC010000555          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZGBC010000555 DAZGBC010000000
VERSION     DAZGBC010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.559"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..424
                     /locus_tag="ACHK1J_005560"
     CDS             <1..424
                     /locus_tag="ACHK1J_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFO7914656.1"
                     /translation="YFIRGMNAPRQVYYDVLIESDTEMVVPDDPIRYRDVHWRLVWED
                     TRYSDGIIPDESEYFLDDAPGKRITCPSGELCPHSGHWATIAGGHQQFIDVQAGQLMP
                     EATKYQSNMYADEIHLPAIWNLLRRDDEGSVYVKTGES"
     gene            484..>788
                     /locus_tag="ACHK1J_005561"
     CDS             484..>788
                     /locus_tag="ACHK1J_005561"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO7914657.1"
                     /translation="MTLFALVDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVM
                     EMNSITDFRERLCELEAALPSVSWMISSSSLTELAAHFRRNMNTELPDGRIALLRFH"
BASE COUNT          198 a          170 c          206 g          214 t
ORIGIN      
        1 ttattttatt cgtggaatga atgctcctcg ccaggtgtat tacgacgtat taattgaaag
       61 tgacacagaa atggtcgtac ccgatgaccc aatccgttac cgggacgttc actggcgtct
      121 ggtctgggaa gacacgcgct acagtgacgg aattattccc gatgagtcag aatactttct
      181 ggatgatgca ccaggcaaac gcattacctg cccgagtggg gagctttgcc cgcattcagg
      241 tcattgggcg accatagccg gaggacatca gcagtttatt gatgttcagg ccgggcagtt
      301 gatgccagaa gcaactaaat atcagagcaa tatgtatgca gatgaaatcc atcttcctgc
      361 gatatggaac cttttacgcc gggatgatga ggggagtgtt tatgtaaaaa caggggaaag
      421 ttaaataact gatcaacgct ttttgacgga cagatctgtt accacctgat ttgctcaaca
      481 aaagtgacat tatttgctct ggtggatggt cagcggtatt tcggtgagag cctgtctgtt
      541 caacaacctg ccgccgtgcc cctgttcgat acctggcctg attcgcgtat tgcatttgcg
      601 gggccatggg ttatggagat gaacagtata acggatttca gagaaagact gtgtgagctt
      661 gaagctgcgc ttccgtcagt ttcctggatg atatcttctt cctcgcttac tgaactagct
      721 gctcatttca gaaggaatat gaatacagaa ctaccggatg gacggattgc gttgctgcgt
      781 ttccatga
//
LOCUS       DAZGBC010000556          782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZGBC010000556 DAZGBC010000000
VERSION     DAZGBC010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.560"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            224..299
                     /locus_tag="ACHK1J_005562"
     tRNA            224..299
                     /locus_tag="ACHK1J_005562"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Ile,seq:cat)
BASE COUNT          229 a          143 c          179 g          231 t
ORIGIN      
        1 attacaaagt atcattgaat cgatgataac tatagatagt gttaattatt aacatttgct
       61 tgttgactgc gagtatcgac tttgcgaatg gaggcgatgt cgggttttat gtcgcatgta
      121 ttgacgaccc aggatggatg agaacgcata aaaatctgtt atttacatca taagatcatc
      181 atgtttattg cgtggtttat aagtattgtt attgcatact taaggccctt tagctcagtg
      241 gttagagcag gcgactcata atcgcttggt cgctggttca aatccagcag gggccaccaa
      301 atcatcattc atgtatcagg aagagttaag ggtacaaggg gtactcgatg gcggtctata
      361 tttcatgact aacgccattt aaaggaagaa gtgcggtggg tggtttcgaa tgagggctgg
      421 tgttttggta cgtggcacgc cactgcaatg tatttatcca tcagtactca ttcaaaagta
      481 cctggaaaaa atgcccgcta tgccaagcgg gcaaattgct tacatgtgga attaagtaat
      541 taacgataaa gccagagtat tatcatttat tatacccatt catttaatca ggttaattac
      601 tcaaatcact gaactattct ctgttcgctc actgatgatg agtgagtacc acaaccaatg
      661 tatgtagaac aatgccgtag agtaaggatg aaatgtctct gccacgcatt aaacatctac
      721 tactgaccag aacacccgcc gcaggtagat gagttttgtg agcgttacag gagcgtggct
      781 at
//
LOCUS       DAZGBC010000557          776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZGBC010000557 DAZGBC010000000
VERSION     DAZGBC010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.561"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..455)
                     /gene="yhaB"
                     /locus_tag="ACHK1J_005563"
     CDS             complement(<1..455)
                     /gene="yhaB"
                     /locus_tag="ACHK1J_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO7914658.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPA"
     gene            complement(711..>776)
                     /locus_tag="ACHK1J_005564"
     CDS             complement(711..>776)
                     /locus_tag="ACHK1J_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO7914659.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          223 a          149 c          152 g          252 t
ORIGIN      
        1 gcagggggtg ttaagatggc ttcttttcgg gtgaattttg cacaatactt agaaatcgtc
       61 tccttaatac attccagctc ttttttattg acggctgagc aagtacattt ttcgccaaaa
      121 ggtgcaaaag tgcctacatc taatgtttgt atgatataat cgtcatgttc tcctgtagaa
      181 atatctatga tgaatggact catattttcc ggttggattc ggatcatgac agaaggaaca
      241 tcgccttgat agaaattcaa tgcagtaatt gcagataata ctctccgcag ttcattactt
      301 atactgtata ttttattaac gtcattggtg gagacaattt ctgcaaactt tctttttacc
      361 gtccaataat caatatttct attgtgattg ttaaccactg cgtgcattgg tgggattgaa
      421 gggtgaaaaa tatgtgcaat tgggaagcct ttcattgatg cctgccttgt catcatgaca
      481 aacaataata gatgcttatt ttcctcgcag ggttctttcg cactatcaaa aaacgctcat
      541 ttacccggcg ttacagtaaa cgaagcaaca ttctgtggcc tacgccgatc aaaagctctg
      601 tgaaaatttc ccttgttgtc tttgtctgac cttatcgcgg cgatcagcaa ttattcgttt
      661 tgtgatcctc aacacaaaat gctattcgtc gcctatgaag ggagaagaat ttatggaaaa
      721 cgattgcatt cacaaaatga cgtcctgtca gaagagaaaa agttgacgtt aatcaa
//
LOCUS       DAZGBC010000558          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZGBC010000558 DAZGBC010000000
VERSION     DAZGBC010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.562"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..542
                     /locus_tag="ACHK1J_005565"
     CDS             <1..542
                     /locus_tag="ACHK1J_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914660.1"
                     /translation="SCVALSACSAQEIAELNRQISDGAFAVTKVLNVGVNQADDDLKY
                     LSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNNGHANGKWAAAA
                     ISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYITYASPAPGHLKD
                     KFIEPLVKRVKDVAEGRVR"
BASE COUNT          244 a          131 c          220 g          180 t
ORIGIN      
        1 cttcgtgtgt tgcgctgagt gcctgttctg cacaggaaat tgcagagctg aacaggcaga
       61 taagtgacgg tgcatttgct gtgacaaaag tactgaacgt aggtgtaaac caggcagatg
      121 atgatctcaa atatctgtct caggcacccc gggcaaaaag agagaaacgt gatcatgtca
      181 tagaagtccc tgttgatgtg gatacagctg cgggaagact gaaacggtac tataagtttt
      241 attcaaatga agaagtcgat gcgattcgta ataacggaca tgcgaacggg aagtgggccg
      301 cagcagcaat aagtcagaac gggcatatgt gggatgccat gcccggtagt tattataaaa
      361 tgggcagtga ctgggatggt ggcgaatttg atgatcatct gacgcttgaa cttgaaaaaa
      421 atggcaaagg aagtcggata tatataacgt atgcatctcc ggctccgggg catctgaaag
      481 ataaatttat tgagccatta gttaaacgtg tgaaggatgt ggctgaaggc cgggtcagat
      541 aaaattaacc cgctggtatt gtcgttgtga tgggctcctc tggaggggcg attatgctca
      601 gaggggctga aagtaaggga cgcagagggc gacgaccagc gacgtctgga agagggcgtg
      661 atatgtaagg aagcacaccc tgacggcata tcataaaaaa accagatatg cgccagttaa
      721 aagtataaat aaagagctga taaaattaac atattttgaa aatgatgaat ttata
//
LOCUS       DAZGBC010000559          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZGBC010000559 DAZGBC010000000
VERSION     DAZGBC010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.563"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..>775)
                     /locus_tag="ACHK1J_005566"
     CDS             complement(113..>775)
                     /locus_tag="ACHK1J_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7914661.1"
                     /translation="NEENPHHVYQPYRLPGQQYDEESGLYYNRNRYYDPLQGRYITQD
                     PIGPVGGWNPYSYVLNNPAQFIDPTGLTQEDNWTYGEVGNSCVTSQRDFTPPPGLADM
                     KASIGAACSRNNASRNCHPVDGSNASAPSDKAAWENIQNATNGKDLSGGGNMFCVGNE
                     DCAVVHSCREIVNARHPAGRVRERVTPLTTSGTATLNGTTVYFYNDPRNGNCPAHEYI
                     PQ"
BASE COUNT          206 a          194 c          159 g          216 t
ORIGIN      
        1 gctttacatt taacaaataa aaataatgtc ataacgcaac atgttataaa aaaccttttc
       61 atgaaaagga tctccaaaaa aggtggctag agcttagtct ggccaccttt ctttattgag
      121 gaatatattc gtgagcagga caattaccat tccggggatc attataaaag taaactgttg
      181 ttccattgag cgttgctgtt cctgacgttg ttaacggagt tacacgttca cgcactcgtc
      241 ctgccgggtg tcgagcatta actatttcac gacagctatg aacaaccgca cagtcctcat
      301 ttcctacaca aaacatattt ccaccaccag ataaatcttt accatttgtc gcattttgaa
      361 tattttccca tgcggcttta tccgatggtg cagaagcatt actaccatct acggggtggc
      421 agtttctgga agcattgttt cttgagcagg cagcgccaat gctggctttc atatccgcaa
      481 gtccgggcgg gggagtaaaa tcgcgttgac tcgtaacaca gctattacct acctcaccat
      541 aggtccaatt gtcctcttga gttaaacctg ttgggtcaat aaactgggct ggattattaa
      601 gcacataact atagggattc caccccccca ccggcccaat cggatcctgg gtaatatacc
      661 tcccctgcaa cggatcatag taccggttgc ggttatagta cagccccgac tcctcatcat
      721 actgctgccc cggcaggcgg tatggctgat acacatgatg cgggttctcc tcatt
//
LOCUS       DAZGBC010000560          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZGBC010000560 DAZGBC010000000
VERSION     DAZGBC010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.564"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..557)
                     /locus_tag="ACHK1J_005567"
     CDS             complement(<1..557)
                     /locus_tag="ACHK1J_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO7914662.1"
                     /translation="MKKRFILSGLTVCLLMAGCQSTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGR"
BASE COUNT          193 a          210 c          215 g          150 t
ORIGIN      
        1 cgacccacaa aaggaacggt gaccggaaca ggagccagct ggctgaccgg taactggtaa
       61 ccgtcacgca gactgtggca gaccaccctc tgcacatcat ccacttccag ttgccatgcc
      121 ggccccgcca ttaactgcag ggcagtacgc agtctcagtg gtcccggctg atactgcacc
      181 gccggcagag gctgacggta cagcacgccg ttggccggtc cggtggcgca gagcgtgtaa
      241 ccggactgtt tcagggcata acgcatggca tcggccaccg tgggtttcag ggaggaggga
      301 atgcgaatgt cgatcacctg agaaagtgga tcacgctgta cagcctgcgg ctctgtgctg
      361 accagcaggt aacggtcata gcgcaccacc tccggtgcct gagaatacac atccgggctg
      421 gcaggctgta cattacggtt tactgtgacc gacggtaccg gcatggccgc atcccgttgc
      481 tgtagtttct gagtgctctg acatcccgcc atcaggaggc agactgtcag tccggacagg
      541 ataaagcgtt ttttcatcgg gtatttacct ctgcagtgat aaatcagagg tcaatgtgca
      601 gaagaagacg gggctggctc aatgaacaac tcattacccg ctggcgaaaa aaaacgcctg
      661 cagacatgca ggcgctttgt tgacaggaaa gaaagcaaca taacaaatat gtcacaatcc
      721 tgcggttaac ataaattacc gattaccgga gagcattacg caaatcat
//
LOCUS       DAZGBC010000561          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZGBC010000561 DAZGBC010000000
VERSION     DAZGBC010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.565"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHK1J_005568"
     CDS             complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHK1J_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7914663.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKE"
     gene            complement(174..377)
                     /locus_tag="ACHK1J_005569"
     CDS             complement(174..377)
                     /locus_tag="ACHK1J_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914664.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(468..>767)
                     /locus_tag="ACHK1J_005570"
     CDS             complement(468..>767)
                     /locus_tag="ACHK1J_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFO7914665.1"
                     /translation="RFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTL
                     NRGEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT          181 a          205 c          172 g          209 t
ORIGIN      
        1 tgttccttca tttcaccgct ctgtttatca cgccaggtat cagtggtggc cagcgtcaga
       61 tttgccactg cacctccgcc gttggggaaa taacggattt ccggatcctg tcccagatga
      121 ccaatcagta tgactttatt cactccacgc gatgccataa caactcctta tcctcacaca
      181 cattcacgct gagtccagat gccgttttcc agagcccgga ctgcctgctc ataatttctg
      241 aaatactctt cggattcccg ggacacaggc ccttcgtcgc actgagtacc aatataataa
      301 ccacgagcac tgctgaggat ctgcagaggt aaggtatagc ccgctttggc ggccagaatt
      361 ccgcttgtcg gtttcataac atgtttcctt aaagttaaac gataagaaaa cagacctccc
      421 tgatggttgg tctgttttca tcagggaggt tgagttgaaa aatacgatca gaaagaattt
      481 tctgcgtatt cccgcggggt attgccattc tgtggcggta tggattccga tttttcagtc
      541 ttataaacca tgtcctggcc tattttgatc cactccacct tgatcaggcg ggctttcaga
      601 ctgacgcgtt gctcaccggc atgttcaccc cggttaaggg taaaaatgtc agtagagggg
      661 ttgctcatgg taaaaccaat cagcaccttg cgatcttcat ccaccgcttt ctggcagcgg
      721 gcaatcaggg aagttgcctc tttaccggca accgtcacat caaaacg
//
LOCUS       DAZGBC010000562          747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZGBC010000562 DAZGBC010000000
VERSION     DAZGBC010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.566"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..226
                     /locus_tag="ACHK1J_005571"
     CDS             <1..226
                     /locus_tag="ACHK1J_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914666.1"
                     /translation="GYNVRYNKAQQLVESCEKIAEGKLPVTCPVCFAIWQDVHRFTHV
                     DFDTQSGKSDFIDTCHSEIITGNVSITGKK"
     gene            383..>747
                     /locus_tag="ACHK1J_005572"
     CDS             383..>747
                     /locus_tag="ACHK1J_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO7914667.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREARQRSV"
BASE COUNT          201 a          227 c          149 g          170 t
ORIGIN      
        1 tggctataac gtcagataca acaaggcgca gcaactggtt gaaagctgcg aaaaaatagc
       61 tgaaggcaaa ctacctgtta cctgtcctgt ctgtttcgcc atatggcagg atgtacacag
      121 gttcacccac gttgactttg acacacaatc ggggaaaagt gattttattg atacctgcca
      181 ttcggagatc ataaccggaa atgtttcaat aacaggcaag aaataaaata atgctccagt
      241 tctgaaatgc aattcactct tataatcccc ccacgaacat gaacgttcat tatcaataac
      301 ccgatgaaat gatattcatc tgaaggccag ttacccgcca cctgtcttat accgtaaccc
      361 gtaatatacc ggattttatt ctatgcaaaa aaaatcactt acactccttc tgccgtcatt
      421 actcagcggc tgtacggcat ttaatgccac gcctgttcct gacaccacac ctccggctct
      481 ggtgttcgtc gacggacaaa ttcgcgaaag cgctgccatt atcgcccata cgcaaaaacg
      541 tctgcttccg ccaccggtga cccgccctgt gacgccgccc cgcccgacaa caacagcgac
      601 gcccacaacc gcagtcagca gccagagtac cgcactgcct ccccgcccgc tccttcaggc
      661 cacacagctg cccgcactgg ttatgaccgg taaacccggt cagtcatccg tcgtttattt
      721 acgtgaggca cgtcagcgct ccgtcag
//
LOCUS       DAZGBC010000563          744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZGBC010000563 DAZGBC010000000
VERSION     DAZGBC010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.567"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..651)
                     /locus_tag="ACHK1J_005573"
     CDS             complement(<1..651)
                     /locus_tag="ACHK1J_005573"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914668.1"
                     /translation="MGSDIPVFYSWTGPFMDSVVSFISAYFPIFLVGAVFGTLMNASG
                     AALTIAHFISDKMGEKRAIIAVIIASFLLTYGGISVFVVVFAVLPIARTIFIKSNYPQ
                     RLLPSAICAGCLPAYVAPGSAQFINAIPIPVFNTTIYSGLVIGIVGTAVWLLFAVWYM
                     SAKIKNAKIKGEGYGTEVSNDCNLETGKVTLAFIPILIVIIGNAFLTYFFQIDEVVD"
BASE COUNT          286 a          145 c          109 g          204 t
ORIGIN      
        1 atcaaccact tcgtcaatct ggaaaaaata tgttaaaaat gcatttccta taataactat
       61 cagtataggt ataaatgcca aagtaacttt tccagtctca agattacagt cattacttac
      121 ttctgtacca tatccttctc cttttatctt agcattttta atttttgcag acatatacca
      181 tacagcgaac aataaccata cagcagtgcc aacgatacca attactaaac ctgaatatat
      241 ggttgtattg aacacaggaa ttggtattgc atttataaac tgggcagaac caggagctac
      301 atatgctggt aagcaaccag cacagatagc actgggtaat aatctttgag ggtaattact
      361 ttttataaat atagttctcg caataggtaa aactgcaaaa accacaacaa aaactgatat
      421 tcctccataa gttaataaaa atgatgcaat aataactgca ataatagccc tcttctcacc
      481 cattttatct gatataaaat gggcaatagt taatgctgca ccacttgcat tcattaatgt
      541 tccaaaaaca gcaccaacaa gaaatatagg gaagtatgcg gatataaaac tcacaaccga
      601 gtccataaat ggaccggtcc atgaataaaa aacaggaata tcacttccca taaagacagc
      661 cagcattgcc atcaaagggg atactatcag aactggaaaa ccacgatata tgagatatat
      721 tagtaaaata agtgaaatta tcaa
//
LOCUS       DAZGBC010000564          743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZGBC010000564 DAZGBC010000000
VERSION     DAZGBC010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.568"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..278)
                     /locus_tag="ACHK1J_005574"
     CDS             complement(<1..278)
                     /locus_tag="ACHK1J_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7914669.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERL"
     gene            complement(336..683)
                     /locus_tag="ACHK1J_005575"
     CDS             complement(336..683)
                     /locus_tag="ACHK1J_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7914670.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
BASE COUNT          162 a          211 c          182 g          188 t
ORIGIN      
        1 aggcgctcca ccgcctgctg gtaattaaat tcgtgtttcg gcttgacgta gcccggacgc
       61 aacacgcggg tttcaccacc acgatgacgc agcacttttc cttcaacaac cggggagaca
      121 taggctgcca ccggcgtttt tccggtaatt ttgtccagca tcacctcttc ggtgtggaaa
      181 ttcaccgtac ggcggaaaaa cagttccaga aacagcgcac gaaatttcac tttttgttcg
      241 gtataaccga gtaactggcg ggtcgtaaac aatcccataa atcagttcct ttcattcaga
      301 aatcagtcag gccaacgcgg tggcctgata acgtgttacg gcagcgccgc gtgactcagg
      361 gctgtgccga caaaggcgtt ggcctttttg tgttcatcca cactttcagg ccagcggatt
      421 gcctccgtcg caaaggtccc cgacttgtaa tacgtcagca ccgtctctgt gccttcaagc
      481 ggcagtacca gtatgccagc cgcactaccg gctttctgtc cgtcccagac caccagtttc
      541 ccgctggctt catccagcat caggggcgtc agtgccggtg ttgcctgaga aatcccgctg
      601 ctgcctgtgg cggtatgagc cggatcatta ccggcaaaaa tacgtacttc tgcacgttgt
      661 tcagtgatgg ttttcgttac catattgtaa aaacctcata ttgatggtca gcactgactt
      721 catggcatgg ccatgagcat ttt
//
LOCUS       DAZGBC010000565          737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZGBC010000565 DAZGBC010000000
VERSION     DAZGBC010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.569"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(549..>737)
                     /locus_tag="ACHK1J_005576"
     CDS             complement(549..>737)
                     /locus_tag="ACHK1J_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO7914671.1"
                     /translation="NTISDRDIKLKGVKDILLSLVDQKANIYSVRQEFLDFLNSDLYK
                     NDGEILSYKESIMMFCAE"
BASE COUNT          246 a          128 c          135 g          228 t
ORIGIN      
        1 atgtagttca aaatatcagg cgttggctaa aagaaaatga aaatatggtg aaagctatta
       61 agaaaattga tactaataac atggcgaata aataaacgga tcaagtcgtt cctgtgacaa
      121 tgaggttata cgaaaaagac gcttacgcat tagaaatctg gtgtacttta attaaaccag
      181 tgtcgctgat gtgtaagcgt cgacgttggg agacgattaa attgttatat caccgctacg
      241 ccctttccag acggcgtata agagtgcaat atgttgagtt ttccgagtga atggtgcatt
      301 ttattaaccg agccatttga ataagccaac gcaataaaac agactggaat aataagtctg
      361 tatttattgt tcatcacaat cgcactctac ggttttatcc tgctgaaatg agacagcctc
      421 cagcgtttgt ggatgatcct tgaacgagag tcatggtctg gtgtcaggat tcacaaatga
      481 cctgtttcct gacatggata taaacccaac atagagtgcg gtagttaatc ttaaaaatct
      541 atagtcggct actcagcaca aaacatcatt attgattctt tataagaaag aatctcgcca
      601 tcatttttat ataaatcact attaaggaaa tccagaaact cttgccttac actataaata
      661 ttagcttttt ggtcaactaa tgataataat atatccttga caccttttaa ttttatatct
      721 ctatcagata ttgtatt
//
LOCUS       DAZGBC010000566          736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZGBC010000566 DAZGBC010000000
VERSION     DAZGBC010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.570"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(522..>736)
                     /gene="subB"
                     /locus_tag="ACHK1J_005577"
     CDS             complement(522..>736)
                     /gene="subB"
                     /locus_tag="ACHK1J_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO7914672.1"
                     /translation="KNSSVWGPSFSTLYNQALYFYTTGQLVRIYYEPGVWTYPPFVKA
                     LTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          214 a          167 c          171 g          184 t
ORIGIN      
        1 acttctgtat taaacgtatc cggatgcatg aacaaatgag caggataatc cggaagactg
       61 gcacagacga aatactcttc cgtctgagta aacaccatca tttatatgag aaaaagaaaa
      121 caaaagaggg gtgagaaacc cattgtatgg ggaaaacttg ccatgctcac tgcgggggca
      181 tttttgtact ggctgttcta accgggtaac ccgttgatac acatagtaca ataatgtttt
      241 atgcaggctt acgcgccaga ctgccatcag tagtgcattg cgtgccagct cgcggacagg
      301 ggcagtgacc agccaccaca tgagtaagca tcatttcagc gccatcaggc agctcctgaa
      361 actcctgcga gttgaaaaag catcctcctg cattagcaat actggcttgt cagtgccagt
      421 gtgatataag acgtaatgtc aaagttcgtg gtgttgacat atttctgtcg caaccaaagt
      481 atgctcccgc ccagtatata tctgggcagg agcatcattt attagttctt tttcctgtcg
      541 gggccaaaac attctgtcga tgtggcgcag gttgataacc caacaagggc attggacgtt
      601 aatgccttca caaagggcgg atacgtccat accccgggtt cataataaat cctgaccaac
      661 tgacctgttg tgtaaaaata tagtgcctga ttatataatg tggaaaacga aggcccccag
      721 acagatgaat tcttca
//
LOCUS       DAZGBC010000567          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZGBC010000567 DAZGBC010000000
VERSION     DAZGBC010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.571"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>722)
                     /gene="tssK"
                     /locus_tag="ACHK1J_005578"
     CDS             complement(<1..>722)
                     /gene="tssK"
                     /locus_tag="ACHK1J_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7914673.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEK"
BASE COUNT          186 a          209 c          193 g          134 t
ORIGIN      
        1 aatttctcag caacctgcca ggccggagta tccgagcgca cagacagcca gaggtccgcc
       61 tcttcgcgca ggcggatatc gtgaaggctg gccttccagg tctgtccatc cgggcgggac
      121 atttccagag caatgacccg ggatggcaga caggcctcca gcagacccgt tatcaggtcg
      181 aacagcggaa gaaacgcacc ggccggatcc ttgtggtcat aacccggtat ggcgtcagga
      241 tcatgatcca gggaaaaagt cagcatactg cctgccagac gagcgagctc cgcccacacc
      301 tgttcaggtg gacgggaagg aaaacgttcg tattccgtca gtacactggc gtgggagttc
      361 agcgcattca gcaaccagaa aagggaaaca tcagcgacag caaaatcggc gagtctttcg
      421 ttactttcac gacgcatcgc catcagacgc tggcgtcggg agcgtaactg acgattgagc
      481 aacaccagac gttcgcgcag aaccggactt gccccaaata acgccaccgg aggaataaaa
      541 gacggatcct gtcgccacgc attctgacca tctctgatca atcttgctac cggacagact
      601 ttccatgaat cattgttttc atgggcaaac cggatggtga aattaaaccg ggcaacggct
      661 atgggctctt cttcagagcc aaaagcatcc tgcagcgtga cccactcttc ccgatagcga
      721 ac
//
LOCUS       DAZGBC010000568          718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZGBC010000568 DAZGBC010000000
VERSION     DAZGBC010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.572"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..540
                     /locus_tag="ACHK1J_005579"
     CDS             22..540
                     /locus_tag="ACHK1J_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO7914674.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            537..>718
                     /locus_tag="ACHK1J_005580"
     CDS             537..>718
                     /locus_tag="ACHK1J_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7914675.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVM"
BASE COUNT          144 a          190 c          227 g          157 t
ORIGIN      
        1 tgaaccacag gaggagcaga aatgaaacga gaaagacagt tttatgtact gagtctgctg
       61 ctgttctgta caacagctgg cgcagaactg aaagtgattg cagacctggg cggcagggat
      121 gccagcccct tttttgaggc gataaaccgg cagccaccgg ttcccgcttc agttgtgcca
      181 tcaccctctt ttccgcaggg agaggcggca atgttaccgg tcagtacgcc tgaaatgtca
      241 cccggagagg ttgtggcgcg ccggcttcag ttaccgggca tcggtgcact ttttcttgtg
      301 ggcgatgatg cccattcccg cacatggctg cagatgaatg cagcagtact cagggagcgg
      361 aaggcggcgg gaatggtagt gaatgtggcg gatgagagca gtctgcgcgt actgcgtgag
      421 ctggcaccgg gggtggtgat ggttccggca tccggttctg tactggcccg caggttacag
      481 ataagtcatt acccggtgct gataacggac aacggcctgt cacaggtacc tgcaccatga
      541 tgccgctgtc tgccacttct gctgcgctct ctgcttctcc ggtactgaca gcctgcctgg
      601 ttctcagcct gctggctgtt gtcctggggc cggcactgtg gcgcagccgg cgaaaaaaag
      661 cgtgtgtgag aacacgccgg catcagcgtt atcgtgccac ggcctcacgg gtaatgaa
//
LOCUS       DAZGBC010000569          717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZGBC010000569 DAZGBC010000000
VERSION     DAZGBC010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.573"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>717)
                     /locus_tag="ACHK1J_005581"
     CDS             complement(<1..>717)
                     /locus_tag="ACHK1J_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914676.1"
                     /translation="DKIEPGKGANRVDGGSGYDTLLLSDVMDDWNIYRVSDGTLFFHS
                     ERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDHRYLLKWRNKNVDYGKHTEGT
                     NGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGNDYLHGASGNDELYGAEGNDWL
                     YGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKGEKDFLFLSKGIVTSTDEFIS
                     CLKQTGNDVEAGIAGGQIYF"
BASE COUNT          209 a          205 c           93 g          210 t
ORIGIN      
        1 cgaaatatat ctgtcctcct gcaatacctg cctcaacatc atttccggtt tgtttcagac
       61 atgaaataaa ctcatctgtc gatgtaacaa taccctttga taaaaacaga aaatcttttt
      121 cacccttatg tttattaaaa tccatgataa atgaattccc ttcacaagca gcatcaaaga
      181 cgaagacatc atgaccagta ccaccataca gataatcatt acctccccca ccatataacc
      241 agtcgttacc ttctgctccg tacaactcat cattaccaga agccccatga agataatcat
      301 tcccgcgacc tccgtgtaaa agacttactc cttctccccc ggcactatgt aatatatcat
      361 ttccgtcaag accaaaaata atatttccga acagactatc atctccgttt gtaccttctg
      421 tatgttttcc gtaatcaaca tttttattac gccacttcag aagatacctg tggtctgtaa
      481 tctgctcttc ttctatctga taagggctta gtttactcct ccagtcacca gaaaaagaga
      541 taccttcaat acctgttgcc tcagtcagac cggctccccg ttctgaatgg aaaaacagcg
      601 tcccatcact gaccctgtat atattccagt catccattac atcgctaaga agcagcgtgt
      661 catacccact gccaccatca accctgtttg ctcccttacc tggttcaatc ttatcat
//
LOCUS       DAZGBC010000570          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZGBC010000570 DAZGBC010000000
VERSION     DAZGBC010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.574"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..605
                     /gene="excA"
                     /locus_tag="ACHK1J_005582"
     CDS             <1..605
                     /gene="excA"
                     /locus_tag="ACHK1J_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO7914677.1"
                     /translation="LARNAYFLLGIPCLALYATAYTSYLLDDKLTHSTSLFILAYGCY
                     FLLFIPPLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQLSFSKSCYFGIDIKNG
                     TMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLPMIPLEISGISSRTLA
                     NTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
BASE COUNT          189 a          170 c          156 g          197 t
ORIGIN      
        1 aactagcccg caacgcttat tttttattag gcattccgtg cttagcttta tacgcaacag
       61 cttatacatc atatttatta gatgataaac taacgcattc aacaagcctt tttattctgg
      121 catatggatg ttattttctt cttttcattc ctccgctctg gctatatctg agaaccagag
      181 cgaaaaaaaa gaaagtccag caggttgttc agcagataaa agaaagcagt ccctttgcgc
      241 cgaccagcga ttacgagcaa ctgtcattct cgaagagttg ttatttcggg atcgacatta
      301 aaaacggcac catgctgtat gtgcgcattt acccaaacaa tgtcatggat gtgattggcc
      361 tggatattca caactttacc cgcacggttg ctgaagacgg caagctggaa atccacacca
      421 cctatgtgag tctgccgatg atccctcttg aaatctccgg catctcatca aggacactgg
      481 ccaacaccat gcacaccatg gctgcacgtg gatatgagta caaggaacgt ttcccgcaga
      541 tgatccggta tcgggttaaa gagtgggaaa aagttgccgg tgtgcctgtt gcagaagtat
      601 tttaaggtag cgaccaactg ttagacggta atccgtcagc cggttttctc tgaaccggct
      661 ttttcttttc aggcagaatg cctgtctgcc gtctatctca ggacgccgca tg
//
LOCUS       DAZGBC010000571          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZGBC010000571 DAZGBC010000000
VERSION     DAZGBC010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.575"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..173
                     /locus_tag="ACHK1J_005583"
     CDS             <1..173
                     /locus_tag="ACHK1J_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO7914678.1"
                     /translation="WGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLD
                     SFTFIDGHVFRR"
     gene            complement(389..609)
                     /locus_tag="ACHK1J_005584"
                     /pseudo
     CDS             complement(389..609)
                     /locus_tag="ACHK1J_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           97 a          228 c          228 g          156 t
ORIGIN      
        1 ggtggggcga ggctgacagt gaggaggctt ttgcggttga gttcgtcagt gataccggcc
       61 tgctggctga cgtgccggag acggtggcgc tctattttga ctatgaggcg tatgcgcggg
      121 atttattcct ggactccttc acctttattg acggtcatgt gttccgtcgg tgatttactc
      181 cccgctccgg cggggggatg gcctgccagg cccccggaaa accctggcag gcttacgcct
      241 gcctgaaata caggagggcc gcgcctgcgg cgctgccctt cctgcccgtc atggtgtgag
      301 gcatttttct gttttgtcgt ttctgttgtt ctgactcccg ggggcgggga cacccttcgg
      361 ctcccggctg cctggcggca tccggtcgtc aggcccgact ccgacaggca gctgctggcg
      421 cacccgccag tctccgccga acccgctggc gcggtttcgg gctgccgcta cggtccgatg
      481 tctgaaaaac caggcctgca gcgaccgttc cggtcgcgcc gcctctgtcg cgccgtgacc
      541 tgcgctccct gcacacctga cggtgtgtaa ggaaactgcg cctgatggcg cagttgtctg
      601 cccttccacc gccacccctg cggcctgttt cccgctgtgg actttccggg ggctgaagcg
      661 gccacaacac gtgtcagccg tccagggggg agatgtacca gccacaaaa
//
LOCUS       DAZGBC010000572          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZGBC010000572 DAZGBC010000000
VERSION     DAZGBC010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.576"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>704
                     /locus_tag="ACHK1J_005585"
     CDS             <1..>704
                     /locus_tag="ACHK1J_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO7914679.1"
                     /translation="QYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFDNLQKN
                     PPPGTCVVACDKFGEALSVFFHRMEKEKLTHMAAIVQSQTHAMAVRLRIKKTPAGETE
                     YVVSFYDPNATNTAVRYKANNCDSFGSLRSFMNIKQAQKKWVITEICSDCVGITPYLP
                     REQAHLLSGINNELQPPLSPSALHLLIQMGMHENIVLFFDKFKNSQEMTASKVLAAKS
                     ITGTYGLYVLWYQNSI"
BASE COUNT          248 a          132 c          123 g          201 t
ORIGIN      
        1 acaatattgt atagattcaa tgaatgaaca tacagggaaa gttccattaa aggcttacta
       61 ttcatctccg gaagatatac aaaaacatat tccattcgag cttgagcagc aatttgacaa
      121 tctacaaaaa aatccaccac ccggtacatg cgttgttgcc tgtgataagt ttggggaggc
      181 attatcagtc ttttttcaca gaatggaaaa agagaagtta acgcatatgg cggcaatcgt
      241 tcaatctcaa acacacgcta tggccgtccg cttgagaatc aaaaaaacgc ctgctggtga
      301 aacagaatat gttgtatcct tttatgatcc taatgcaacc aatactgcag tacgctataa
      361 agcaaacaac tgtgattctt ttgggtcatt gcgatccttt atgaatatta aacaggcaca
      421 aaaaaaatgg gtaatcacag aaatttgctc cgactgtgta ggaataactc cttatctccc
      481 tcgagaacaa gcccatttat taagtggtat aaataatgag ttgcaaccac cattatcacc
      541 atcagcatta catctattaa tacaaatggg gatgcatgaa aacattgttc ttttttttga
      601 taaatttaaa aactcccaag aaatgacagc atcaaaggtt cttgctgcga aatcaattac
      661 aggaacatat ggattatatg tattatggta tcaaaatagt attg
//
LOCUS       DAZGBC010000573          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZGBC010000573 DAZGBC010000000
VERSION     DAZGBC010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.577"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            148..573
                     /gene="yqeK"
                     /locus_tag="ACHK1J_005586"
     CDS             148..573
                     /gene="yqeK"
                     /locus_tag="ACHK1J_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO7914680.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRYVNVNSVSKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLILSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            609..689
                     /locus_tag="ACHK1J_005587"
     CDS             609..689
                     /locus_tag="ACHK1J_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914681.1"
                     /translation="MKDVDQNFDALYWHILQSYLILLFYD"
BASE COUNT          234 a           95 c          132 g          243 t
ORIGIN      
        1 tcatttgatc aaggttatca ccttatggag acgttacgta gaatgactat atttgttatc
       61 ggcacatttg gggtattaat tttagtattg atggaaggtg attattgaac agtaacatcg
      121 agtcacggta aaaatgtgag gtatataatg gacattgagt tttcgcagat tcatgagatg
      181 gtctatatgc atgatatagt taattcggac tcgaaaaaga aaccaaggat tccgttaaaa
      241 aaattcctta atgctgagaa ggttctcacg caaacaacat catgggcatt aaattcaaga
      301 tatgtgaatg ttaattctgt cagtaaagtc aacgtaaaga gtaaagttaa aaacagttat
      361 atctcacgat ctgtcaatga tgagtttagt ttaacagatg atgaaattaa ttcttttaaa
      421 gaaacgttaa tcctttcctc tattgattca ctgagtaaac tggttcttaa taatcctctt
      481 tctgtattgt ttacatcgac tgtacgaagg aataataatc gagctaagat gaatgttgag
      541 ttcgactcat ggatatgcac cagatgttgt tgatacatga tattttacat tgcaaggtga
      601 tgtctaaaat gaaagatgtt gatcaaaact ttgatgcttt atactggcat atactgcaat
      661 catatttaat tttattattt tatgattaac gaggttccat cttt
//
LOCUS       DAZGBC010000574          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZGBC010000574 DAZGBC010000000
VERSION     DAZGBC010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.578"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>698
                     /locus_tag="ACHK1J_005588"
     CDS             <1..>698
                     /locus_tag="ACHK1J_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO7914682.1"
                     /translation="KGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAALIRNGEE
                     RGNIAEALFQVTKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIPIVSAFA
                     PEEKWSFQMKVLAAMSELVIGYGLWISGAIITLVIIIRFSLSRYTGPGRRLLDKLPPW
                     SLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKNVLSGQN
                     LATAMRNSDYDFPS"
BASE COUNT          180 a          181 c          165 g          172 t
ORIGIN      
        1 aaaggaacat ccggcatgta ctttctggcc agagaatgta ttgcctccct gaaagccagc
       61 ggtatgctgt cgggggccct gcccacatgg gttcccccgg aagaagcggc actgatcagg
      121 aacggtgaag aacgcgggaa catcgccgaa gccctgttcc aggtcacaaa aaccgtgaaa
      181 gcacggcggg aaatggtgag cagtctcatc acactctgtc tttatcccct tctgctgctt
      241 tcactctgtg tggtgaatat gtacaacgca catacccgca tcatccccat tgtcagcgct
      301 tttgcaccgg aggagaaatg gtcattccag atgaaagtcc ttgcagccat gtcagagctt
      361 gtcatcggct atggtctgtg gatcagtggt gccatcatca cgcttgtcat catcatccgc
      421 ttctcactca gtcgctatac cggcccggga cgcaggcttc ttgacaaact gccaccatgg
      481 tcgctctatc agacttttca tggtgtgaat tatctgttca gcatatcgtc aatgctgcag
      541 ataaatattc cgctttcaca ggcgctgctc agaattgaaa aaagtgcaga gcataataaa
      601 tggttaaagg agagaattca ggcaataaga aaaaatgtat tatccggtca gaatctggcc
      661 accgccatgc gcaattctga ctatgatttt ccttcaaa
//
LOCUS       DAZGBC010000575          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZGBC010000575 DAZGBC010000000
VERSION     DAZGBC010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.579"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>698
                     /locus_tag="ACHK1J_005589"
     CDS             <1..>698
                     /locus_tag="ACHK1J_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HFO7914683.1"
                     /translation="PEGCRHAIMEQIKYCQSHLQQINNGPKRVLILGASSGFGLAARI
                     SLTFGGAKCDSIGVSFEKGPSEKSIGTAGWYNNIFFKEFAEAEGRIAHNVIGDAFSDD
                     VKNKVLNLINKDFDGEVDLIIYSLATGQRSDPATGNIWRSCLKTTGSAFTGKTVNIED
                     NKLESTIIDVASEAEIADTIKVMGGDDWESWILWLKENNVLTKKTKTIAFSYIGPEMT
                     YPIYHTGTLGKAKQ"
BASE COUNT          224 a          111 c          156 g          207 t
ORIGIN      
        1 atccagaggg atgtcgtcat gcgataatgg aacagattaa gtattgccag tcccatcttc
       61 agcaaattaa taatggcccg aaacgagttc tgattctcgg tgcatcttca ggatttggtc
      121 ttgctgcaag aatttctctc acttttggcg gcgcaaagtg cgattctatc ggagtttcgt
      181 ttgaaaaagg tccctctgag aaatctatag gaactgcagg atggtataat aatatattct
      241 ttaaggaatt tgctgaagct gaaggcagaa ttgctcataa tgttattggc gatgctttct
      301 ctgatgatgt aaaaaataaa gttcttaact taataaataa ggattttgat ggcgaggtcg
      361 atcttattat atatagtctg gccacagggc aaaggtcaga tccagcaaca ggtaacattt
      421 ggcgttcctg tttaaaaacg actggcagtg cttttacagg gaaaacggtt aatatcgaag
      481 ataataaact tgaatcaaca attattgatg ttgcttcaga ggctgaaatt gcagatacta
      541 taaaagttat ggggggggat gactgggagt catggatact ttggttaaag gaaaataatg
      601 ttcttactaa gaagaccaaa acaattgctt tttcatatat cggtccagaa atgacttatc
      661 cgatttatca tacagggaca ttaggaaaag ccaaacaa
//
LOCUS       DAZGBC010000576          694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZGBC010000576 DAZGBC010000000
VERSION     DAZGBC010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.580"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>694)
                     /locus_tag="ACHK1J_005590"
     CDS             complement(<1..>694)
                     /locus_tag="ACHK1J_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO7914684.1"
                     /translation="DFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPTKFHECFIN
                     WMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSN
                     EITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEE
                     KFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSF
                     RSIIAEQKKEPEM"
BASE COUNT          187 a          163 c          121 g          223 t
ORIGIN      
        1 catttctggc tctttctttt gctctgctat tatggaccga aaggagactg ccacgcataa
       61 tttcttcagc cctttccatt caaacgtgaa atcaataagt tcatcaggga catcgcaaac
      121 aatatgaaga cggatttctt ctctgccgtg actcttttca ctaattgcgt aactgtcatg
      181 ctctggatta tttaattctt tcagcggaaa tttttcctca aaggctttat taagccgccc
      241 ctggttacct tttaccgcga ataaataatc accgccctgt ttttgtatct tctctgcgat
      301 atctttctgg caacccatcg catcagttgt gataattttc cctttaatat ccagcatgtt
      361 aagaagttca gggatagctg taatctcatt agatttctca tccgtcttga tctgtccgat
      421 gaccagactg tgcattgttg agaacgcact aatgacatga atcgctcccc tgcggcgact
      481 cttgtcataa gaatgccgga gcgtttttcc atcaattgca atgacgtctt tatcatctga
      541 agaatgacaa tcacgcatcc agttaataaa gcactcatga aattttgtag ggctgataca
      601 ggatacaact ctggcaatgg tatcgtgaac aggaatacca ttttcaaaat caccatattg
      661 cttcaaaaaa tcgagatgtg tttccccaaa atcc
//
LOCUS       DAZGBC010000577          694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZGBC010000577 DAZGBC010000000
VERSION     DAZGBC010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.581"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..170)
                     /gene="ynfQ"
                     /locus_tag="ACHK1J_005591"
     CDS             complement(<1..170)
                     /gene="ynfQ"
                     /locus_tag="ACHK1J_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO7914685.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSRLS"
     gene            complement(181..393)
                     /gene="cspI"
                     /locus_tag="ACHK1J_005592"
     CDS             complement(181..393)
                     /gene="cspI"
                     /locus_tag="ACHK1J_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO7914686.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSVIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          248 a          133 c          111 g          202 t
ORIGIN      
        1 gacagacggc taacatttaa gtattgtgaa atattatcaa atgtaatcat cattgattta
       61 caaaagatac attttgcccc gaaaggattc ctgtcagaaa catcaaaaga tgatgttcta
      121 tactgagaac catggcaaca cgggcatcta aagtgaatat ggtttgtaat attttctacc
      181 tcaaagtgcc actacatgaa cagccgcggg gcctttaggt ccgttctcaa taccaaattc
      241 gacttcctga ttctcagtta acgttttgaa atcgttgctc tgaataactg agaaatggac
      301 aaacacatct ttgctaccat ctttcggcgt gatgaaacca aaaccttttt cagggttaaa
      361 ccatttcact aaaccagtca ttttgttaga catcattatt accttttgaa gaagttagcc
      421 cttgggcaga atggtccgaa aaaaaatatc agagagaaaa accaacaagg agatctcaag
      481 aggtacaaat aataaaatta taacaatgac tgcttcagat aattttgtaa caaaccagaa
      541 caccattaac gcatgattaa ccaaccatag caaggattac ttttgtaaag aaaaacacac
      601 gaatgaaaca atagctttat ttattaataa aacgtgtcat tctgtctaat gaccttttat
      661 cttaccctta agatttcaag gattttgact catg
//
LOCUS       DAZGBC010000578          693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZGBC010000578 DAZGBC010000000
VERSION     DAZGBC010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.582"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            291..691
                     /locus_tag="ACHK1J_005593"
                     /pseudo
     CDS             291..691
                     /locus_tag="ACHK1J_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          203 a          144 c          161 g          185 t
ORIGIN      
        1 tggaaacatt tacactcctg tttaataaat tatattgccc agggcgcatt ctaaaacaca
       61 ataaaattac cactatcaaa aatcgctcat atccatcgcc cggaatatga ataaaaacat
      121 caacaaagat taaattattc aaacgttact gttgcgtcag tggtcgtagc aacatccgac
      181 gtggtgatgt gtgttaaagg agacgctctg atgttcaggg gggttatcgc gtatacggat
      241 cgtggatggc agaaaacgtt caggatcagg tatccatttt aaaccagata atgagtaagt
      301 ttgccccatc catgccccac gcggtgagat cggatgtcat taaaaaagtc aggtcagcag
      361 gtcagtatgg gaaggcgaac aactcgcagg aatgggtgcg ctgaagttgc tggatgataa
      421 acatggcgag ctgaaatcaa tgcgtaccgc gccaaattat ttacgtcggg gtgtcgccag
      481 tctgatttta cgccacattt tgcaggtcgc ccatgacaga tgccttcatc gcctgagctt
      541 agaaacgggt acacaggctg gatttacggc ctgccatcaa ctttatttga aacatggatt
      601 cgttgattgc gaaccgtttg ctgattatcg atttgatcca cacagtcgat ttttatcatt
      661 gacgttatgc gaaaataatg aattgccatg agc
//
LOCUS       DAZGBC010000579          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZGBC010000579 DAZGBC010000000
VERSION     DAZGBC010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.583"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>692
                     /locus_tag="ACHK1J_005594"
     rRNA            <1..>692
                     /locus_tag="ACHK1J_005594"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          156 c          229 g          132 t
ORIGIN      
        1 gctggtctga gaggatgacc agccacactg gaactgagac acggtccaga ctcctacggg
       61 aggcagcagt ggggaatatt gcacaatggg cgcaagcctg atgcagccat gccgcgtgta
      121 tgaagaaggc cttcgggttg taaagtactt tcagcgggga ggaagggagt aaagttaata
      181 cctttgctca ttgacgttac ccgcagaaga agcaccggct aactccgtgc cagcagccgc
      241 ggtaatacgg agggtgcaag cgttaatcgg aattactggg cgtaaagcgc acgcaggcgg
      301 tttgttaagt cagatgtgaa atccccgggc tcaacctggg aactgcatct gatactggca
      361 agcttgagtc tcgtagaggg gggtagaatt ccaggtgtag cggtgaaatg cgtagagatc
      421 tggaggaata ccggtggcga aggcggcccc ctggacgaag actgacgctc aggtgcgaaa
      481 gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg atgtcgactt
      541 ggaggttgtg cccttgaggc gtggcttccg gagctaacgc gttaagtcga ccgcctgggg
      601 agtacggccg caaggttaaa actcaaatga attgacgggg gcccgcacaa gcggtggagc
      661 atgtggttta attcgatgca acgcgaagaa cc
//
LOCUS       DAZGBC010000580          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZGBC010000580 DAZGBC010000000
VERSION     DAZGBC010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.584"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>691
                     /locus_tag="ACHK1J_005595"
     CDS             <1..>691
                     /locus_tag="ACHK1J_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO7914687.1"
                     /translation="LPQPDTVFSGESLLPPPVLSTPGRCPCCRRTVTHRFILEDSWPL
                     QQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHTRTLQDAERSLAQARLSAR
                     RLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCHAWHCLNGYAAAQGVMVWL
                     PDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQNRLAVEEKFRTWRPADFAD
                     ALRRWPPAQRQM"
BASE COUNT          155 a          216 c          193 g          127 t
ORIGIN      
        1 gctcccgcaa ccggacacgg tattcagcgg tgaatccctg ctgccaccac ccgtgctctc
       61 aacacccgga cgctgtccct gctgtcgcag gaccgtcact caccgcttca ttctggagga
      121 ttcatggcca ttacagcaga tggctgacac ctgccgggat acggtcgtcc tgctggaaaa
      181 aaacctcacc cgggtgatgc gcctgaaaaa acatccggta ccggaaaacg ctgacgaaaa
      241 aaagaaacac acccgaaccc tgcaggatgc ggagcggtcc ctggcacagg cccgcctgtc
      301 ggccaggcga ctggccctgc gtcatgtgga aaagtcacaa atcgtcacaa cagatgcact
      361 cagcgaaaat gaaagtgaac tgctgcagcc tgaaggcccg ccctttcatc tctgtgcctt
      421 ctgccacgcc tggcactgcc tgaacggcta cgctgccgca cagggggtga tggtctggct
      481 ccctgatttg catccggcct cggtagttgc tctcaatgca cgggcactga aggaaatttt
      541 cagcgatgag cgtaagcgaa tcagacaggg acgggccgta ctgaatgccc tggtacagaa
      601 ccgcctggcc gttgaggaga aatttcgcac ctggcgtccg gcggattttg ctgatgcact
      661 gcgccgctgg ccgccggcac agcgtcaaat g
//
LOCUS       DAZGBC010000581          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZGBC010000581 DAZGBC010000000
VERSION     DAZGBC010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.585"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..429
                     /locus_tag="ACHK1J_005596"
     CDS             4..429
                     /locus_tag="ACHK1J_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7914688.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            476..>689
                     /locus_tag="ACHK1J_005597"
     CDS             476..>689
                     /locus_tag="ACHK1J_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7914689.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIV"
BASE COUNT          152 a          169 c          202 g          166 t
ORIGIN      
        1 cccatgcagt atgcgaaacc tgtcactctg aacgttgaag agtgcgaccg tctgtctttt
       61 ctgccttacc tgtttggcct ggattttctg tatgccgaag cgtctgtata cgcgctggcg
      121 aaaaaaatga tgccggaata tgaaggcgga ttctggcact tcatccgtct gccggacggt
      181 ggcggttaca tgatgccgga tggcgaccgt ttccacatgg tgaacggtgc aaactggttt
      241 gaccgtaccg tgagtgctga tgccgcaggc atcatcctta cctcccttgt gattaaccgc
      301 cagttgtggc tgtaccacga cagcggggat gcagggttga cacacctgta caggatgcgc
      361 gatgcgcagt tgtggagcca catcgaattt caccccgaat gcaacgcgat ttacgcggca
      421 cttgactgat taacggacgg ggcggcaatg ccgcccctgt aaaggagacg agaacatgta
      481 ctgtactgtt aaagaaatca tccgcgatgt actggataca gacgtgccgg acagtgaatg
      541 cgtttttgcc gtggtgctga cccgtgggga tgtgcgccac atagcccagg actggagtct
      601 gacagacgat gagctggaaa ccgtcatgca gcggctggac gatgcttttg tatatggcgc
      661 atgtgacaga gttgtttccg atattgtca
//
LOCUS       DAZGBC010000582          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZGBC010000582 DAZGBC010000000
VERSION     DAZGBC010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.586"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>684
                     /locus_tag="ACHK1J_005598"
     CDS             <1..>684
                     /locus_tag="ACHK1J_005598"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFO7914690.1"
                     /translation="RERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQVIVWL
                     EKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKREKTPPP
                     LCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHEESSEV
                     LEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGDELKVY
                     QLIRQHYLA"
BASE COUNT          147 a          158 c          209 g          170 t
ORIGIN      
        1 acgggaacgt ggaatttctg gggttttgtc agtgggccgg gtgcagacac ccacgctggc
       61 aatggtggtc agacgggatc aggagatcgc cacatttgtg ccggtttcct ggtggcaggt
      121 gattgtctgg ctggaaaagg aggggctccg tttccgcagt aactgggtgg cagctgagca
      181 gtactgcgat gatgagggac gttgtgtgaa tgtgcaggcg gccggtgctg ttctcggtct
      241 gtgccgtcag cagtcctctg ctgttgtaca ggatgttgta cagaagaggg agaaaacacc
      301 gccacctttg tgtttttcgc tgggaacgtt gcaggaagcc tgttcccgga agtttggcat
      361 gggggctcag accgttctgg atatcgcaca gtccctttat gagactcaca aagcaacaac
      421 ttatcccagg acagactgtg gttatctgcc tgcctcgatg catgaggaga gttctgaagt
      481 gcttgaggct gtcgggcgtt cagatccgtc cgtgcttcca caactggctg aactggaatc
      541 aggacgggta tcccgtatct ggaatgataa aaaaattacg gctcaccatg ccattattcc
      601 gacccggcag gtttttgacc tcagctcact gaacggtgat gaactgaagg tatatcaact
      661 gattcgtcag cactatctgg caca
//
LOCUS       DAZGBC010000583          681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZGBC010000583 DAZGBC010000000
VERSION     DAZGBC010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.587"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..300)
                     /locus_tag="ACHK1J_005599"
     CDS             complement(<1..300)
                     /locus_tag="ACHK1J_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914691.1"
                     /translation="MKVVFNVKSDFDKIFLPLEFSGFHGRNGYCYLRVQIKRGFIVFS
                     CAQLLNYYRTSVTNAIEQVREAAVNVLFREGVLSYTQQKEFWDLLKTSQRVNKEID"
     gene            complement(478..>681)
                     /locus_tag="ACHK1J_005600"
     CDS             complement(478..>681)
                     /locus_tag="ACHK1J_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO7914692.1"
                     /translation="RMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVL
                     LTPGHYLGNWDHRLRHLQEQNVA"
BASE COUNT          214 a          171 c          120 g          176 t
ORIGIN      
        1 atcaatttct ttgtttaccc gttgtgatgt ctttagtaaa tcccagaatt ctttttgctg
       61 tgtgtatgac aacacacctt ccctgaagag gacattaacc gcagcctctc tgacctgctc
      121 aattgcattt gttactgacg tacgatagta gttcaataat tgagcgcatg agaaaacaat
      181 aaaaccacgc ttaatctgaa ctcttaaata acagtaccca ttccgcccat gaaaaccaga
      241 aaactccagt ggtaaaaaaa tcttatcgaa atcacttttt acattaaaga caaccttcac
      301 ttaactaaac tcctgtttga tatcttgaga atataattaa tcctgagttt ataaaatgaa
      361 tagcgatgac caagctgtca acatggcata cataaactta aaaaaaacaa caaaaactga
      421 cgttgcagat gtgataacgc cagtccgaag actggcgctg tgataaaaca ttctgcatca
      481 tgccacattc tgctcctgaa gatgacgcag acgatggtcc cagtttccca ggtaatgccc
      541 gggagtcagc agtacgcggc gtcgctcatt acagagcgag ttaccaatca ccacttctcc
      601 ggccacacca cacagcgaca gttgtatata cgccatgccg gctgccagcg ggtcaatatc
      661 cgttgccgac acccacatcc t
//
LOCUS       DAZGBC010000584          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZGBC010000584 DAZGBC010000000
VERSION     DAZGBC010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.588"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>675)
                     /locus_tag="ACHK1J_005601"
     CDS             complement(<1..>675)
                     /locus_tag="ACHK1J_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7914693.1"
                     /translation="VINCDKLKSLPSTKNWTYATYFRFIIADYFYNKHEKILYLDADI
                     ACKGSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYFNAGFLLINID
                     EWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISGKYNTRYSINYELK
                     DKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKASSPWCKEDLLKPVNSNQY
                     RYCAKH"
BASE COUNT          215 a          127 c          105 g          228 t
ORIGIN      
        1 ttatgttttg cacaataccg atactgattg ctatttacag gtttaagtaa atcttcttta
       61 caccacggag aagatgcttt ggcgatcaaa aaactacgtg acaccggata gtcagcccat
      121 tcatgccagg gttttgtagg tcccacatag tgtataaaca cggtatcatc attgactgga
      181 ttatcaactt tgtcttttaa ttcatagtta atactatatc gggtattata tttcccagaa
      241 ataaacttca ctttaccatt caataataca ttcagtacat cttgatcaag gtgggtgatt
      301 ttacttaccc aatctggatc acgcaacatt tcaatagctt ttgacgaaat gttattaaga
      361 ttccactcat caatatttat cagtaaaaaa ccagcattga aatatccaga agctaattgt
      421 ggtgtagtta ataccgaggc tcgattctgc caccattcaa catctctttc agctacaact
      481 gcggcaattt cattagtaga gaattgataa tctaagagtt ctttaatact acccttgcaa
      541 gcaatatctg catcaagata tagtattttt tcatgtttat tatagaaata gtctgcaatt
      601 ataaatcgaa aatatgtagc gtaagtccag tttttcgtgc ttggtaatga cttcagctta
      661 tcacaattga taaca
//
LOCUS       DAZGBC010000585          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZGBC010000585 DAZGBC010000000
VERSION     DAZGBC010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.589"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            291..>670
                     /locus_tag="ACHK1J_005602"
     CDS             291..>670
                     /locus_tag="ACHK1J_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO7914694.1"
                     /translation="MVDNVTVGGVCAQSPSFMPDLDGGKNKAQLIIDDIVAYLKNPSV
                     YSLEKEGPLNHFVNNCSEIEPWVLIGGGLIIIVSTSEQDPEGLIVTVGDIEGKCATQI
                     PVPPALTTSLKDIFIRPHTNAKYE"
BASE COUNT          208 a          110 c          137 g          215 t
ORIGIN      
        1 ttgtgatagc tggcccatac aaaatattag caaaaatcca tgaatggata aattttttaa
       61 tttttatcat tattgttgaa gatgatggca ttgattatag gggttccatt acaaggagct
      121 gtttcccaca ttgacgtccc ggtttaccca cttcattacc aggttgatga cctgatgggc
      181 taatgacgta aaaagacaaa taactctata aatgagcgaa atttgatagt tgatttttaa
      241 atagtaacct tattattact acaactttca tatggatgag gggaaagaaa atggttgata
      301 acgtaactgt tggcggtgta tgtgcacaat ctccttcttt catgcctgat ttggatggag
      361 gaaaaaataa agctcaattg atcattgatg atatagtggc gtatcttaaa aatccttcag
      421 tttattcgct tgaaaaagaa ggtccgttaa atcattttgt taataattgt agcgagattg
      481 aaccatgggt tttaatcggt ggtggactta ttattattgt ctccacatct gagcaagacc
      541 ctgaaggtct gattgtaact gtgggggata ttgaaggaaa gtgtgctaca cagattcccg
      601 taccacctgc gcttacaaca tccctcaaag atatttttat tcgccctcat acgaacgcaa
      661 agtatgagag
//
LOCUS       DAZGBC010000586          669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZGBC010000586 DAZGBC010000000
VERSION     DAZGBC010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.590"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>669)
                     /locus_tag="ACHK1J_005603"
     CDS             complement(<1..>669)
                     /locus_tag="ACHK1J_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO7914695.1"
                     /translation="LEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDVKE
                     NVDRALLRMFKCVELHNNELDLSYLFWGSVPPLPEWIEMLYLSDNQLDSVQVPESCKE
                     LGLDSNNLTEFPQVPDGITLISVNNNLISHIDSFPPKIKEIYIRHNKLSQIPEIPDTT
                     TVFDCGCNKIQEIRYFPKNLEKALIEYNNIEVVPAIHSKLKLLFIECNPIKEAFLMPW
                     TLTD"
BASE COUNT          193 a          129 c          124 g          223 t
ORIGIN      
        1 tgtctgtcaa agtccatggc attaaaaatg cctctttaat aggattgcat tctataaaaa
       61 gtaatttcaa cttgctatgt atcgcaggaa caacctcaat gttattatac tcgataagtg
      121 ctttctccag atttttgggg aagtatcgaa tttcttgtat cttattacag ccacaatcaa
      181 aaaccgttgt agtatctggt atttctggta tttgcgatag cttattgtgc cgaatataaa
      241 tttccttaat cttcggcgga aatgagtcaa tatgcgatat caggttatta tttacggaga
      301 tcagggtaat tccatcaggt acttgtggaa attctgtaag gttattggaa tcgagtccta
      361 attctttgca tgattcgggc acctgtactg agtcaagttg attatcacta aggtaaagca
      421 tttcaatcca ctctggaaga ggcggtacag aaccccaaaa taggtatgat aagtcaagtt
      481 cattattgtg gagctcaaca catttaaaca ttctcagcaa tgccctatcc acattttctt
      541 tgacgtcatt cgatgcgcct tctttgactg aactagccca gtcactgaaa cctttgcaaa
      601 gcctggcatc tgtaattttg gttaacttgt ccagcaactc tgcaatattg gtcgatataa
      661 actctaatt
//
LOCUS       DAZGBC010000587          669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZGBC010000587 DAZGBC010000000
VERSION     DAZGBC010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.591"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>669
                     /gene="espY3"
                     /locus_tag="ACHK1J_005604"
     CDS             <1..>669
                     /gene="espY3"
                     /locus_tag="ACHK1J_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFO7914696.1"
                     /translation="FYKDGENLMIRGTISIVNEVVKTYSLPIEKDDNGYYDFSGLYLA
                     HSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNANCTNANFNNCRFKK
                     CDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKSDGNETKGMILTNCS
                     CVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYRTDLFNPILKTEAES
                     TEEKD"
BASE COUNT          251 a          113 c          125 g          180 t
ORIGIN      
        1 ttttataaag acggagaaaa tctgatgata cgtggaacta tctctattgt taacgaggta
       61 gtaaagactt attcactccc aatagaaaag gatgacaatg gatattacga tttcagcggt
      121 ctatatctag cacacagcaa tatttccggt aaagatccca ataaagatcc cgatatagat
      181 ttcggtatag atatgggaaa ctgtaactgc tcaaatgtca acttcgaaca tacttatttt
      241 tatggcgtca agtttacaaa tgcgaattgt acaaatgcga attttaacaa ttgtagattt
      301 aagaaatgcg acctgacaaa tatgaattgc actggcgcaa ttcttgataa tgcgatgata
      361 tatggtaaag aaaaagaacc tgagatgcaa tatccagaag cagatcaaat aatacaaagg
      421 ataacttatc agaaatcaga tgggaatgaa acgaaaggga tgattctaac taactgctcc
      481 tgtgttaaaa ccactttcaa ttgggcagat ttatcagaaa gtgattgcca aaatgtggac
      541 tttagcgagg ctaatctttc aaacactatt cttcctgaca tcgtcaggat gaaaggcacg
      601 aaactttacc gtaccgacct ttttaatccg atattaaaaa cagaagcaga atcaacagaa
      661 gaaaaagac
//
LOCUS       DAZGBC010000588          666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZGBC010000588 DAZGBC010000000
VERSION     DAZGBC010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.592"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..449
                     /locus_tag="ACHK1J_005605"
     CDS             <1..449
                     /locus_tag="ACHK1J_005605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914697.1"
                     /translation="RLQQIASSTNDNNLKDTAMELTVDLLKSLNNIRQNATIYVHAYH
                     YTDDEKCAMVPRISLPSLKLTPLQSEIIIRLLLNGVDYIRHDGYVQGYREKTVATFTI
                     QGEGRHRFKADLSQNGESDIASNVLNHWLHRIHKTDIIVLSVVLTE"
BASE COUNT          232 a          133 c          126 g          175 t
ORIGIN      
        1 aacgccttca acagatcgca tcatcaacga atgataacaa cctgaaggat accgcgatgg
       61 agttgactgt agacctgctg aaaagcctta acaacatcag gcaaaatgcc accatatatg
      121 ttcacgcata ccactatact gatgatgaaa aatgcgctat ggtgccacgg atatctttac
      181 catcactgaa attaactccg ttgcaatcag aaataattat tcgactgctt ctcaatggag
      241 tcgattatat aaggcacgac ggatatgttc aggggtatag agaaaaaact gtggcaactt
      301 tcacaataca gggggaggga cgccatcgtt tcaaagcaga cttatcacaa aatggggaaa
      361 gtgatatagc cagcaatgta ctaaatcact ggctacatcg tatacataaa acagatatca
      421 ttgttctgtc tgttgttctt acagaataaa tcctgttacc tcctgaatgt tacaaggttt
      481 tatatttatc ctatgagcat acgcacgtga aaactaataa aaaaggcaca aaatggcaca
      541 ttttttaccg tgaaaattca ggcgcggaag ttttgcttga aataccatca tttcgggaat
      601 gtctttcagt atccaaagaa ttaatgaccc catcaaatta catgatatgc atagaaaaaa
      661 atggcg
//
LOCUS       DAZGBC010000589          664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZGBC010000589 DAZGBC010000000
VERSION     DAZGBC010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.593"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(147..335)
                     /locus_tag="ACHK1J_005606"
     CDS             complement(147..335)
                     /locus_tag="ACHK1J_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO7914698.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQAECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
BASE COUNT          172 a          202 c          121 g          169 t
ORIGIN      
        1 gttgatttca tgacgaataa aatgaataaa ttctcccgtg gtctttacca ccattattat
       61 accaataaca ccatttacac agatacactc cgggactatc tgtttttgcc ccctccgact
      121 ccgccggagg ttttttattt tcatactcag gccttcacca cttcacaccg gaagtcctcc
      181 accctgacgc caatataccg tcccggctct tttgcgggcg caaacagttt ttcacattca
      241 gcctgagtca ttttttcatc ctggtaacgc agccactgaa ccggcatatt gcctggtttg
      301 attattgtcg cagttatttt atatcgcttt cccatactgg ctctcatctc aacaacaaaa
      361 tcaccggctg agccatcact ccggtaatgc atgcgaaaaa aagtgagcgt ctcagcaatg
      421 gctcatgcgg tgattctgta cggtacccgc acaaaatacg ggcaccaaca gcgtcgcaga
      481 atacaacgaa ggcatcaggg cagcacctct ctcccgctat ttttcagagt tctttaccct
      541 cctcctgaca cgggagtaca accctgacag catccgggcc agtacattcg caagcaccca
      601 tgaaaacaca gccacaccca cagctcccgc cagaaacagc acaggttcac tggcaatttg
      661 atct
//
LOCUS       DAZGBC010000590          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZGBC010000590 DAZGBC010000000
VERSION     DAZGBC010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.594"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..>663
                     /locus_tag="ACHK1J_005607"
     CDS             137..>663
                     /locus_tag="ACHK1J_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFO7914699.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVL"
BASE COUNT          185 a          154 c          150 g          174 t
ORIGIN      
        1 atgacagagt tgtaacatag aatctaaaag catgattatc gtcaaaaaat aatggaatta
       61 ttttttcctt tttgttgaaa tcgaaattat ataaccttag tgtaaacaaa aatcacacgg
      121 tcttctgtag gaggatatct ctatgaacaa cacagtcagc agtgagcaaa aaacgctgga
      181 cattatctgt ctggggcgtg tcgccgttga tttatatggt caacaaatcg ggtcacgtct
      241 ggaagacatg acaacctttg caaaatattt aggaggctca tcaggcaacg tcgcttacgg
      301 cacagcaatt cagggactac gttccgccat gctggcacgt gtgggcgatg aacatatggg
      361 acgtttcatc cgcgaagaat taaaccgtgt tggcgtcaat acacaatgcc tgttgactga
      421 tcctgaacgt ctgacagccc tggtgatcct tggtattaaa gatcaggaca ccttcccgct
      481 gatcttttat cgagataact gtgcggatat ggcgctggcg cctgaggata tccacgaaac
      541 attcattgcc tcttcacgtg ccctggccgt caccggcact catctttctc acatcaatac
      601 ccgcgccgca gtgcttaaag cgctgaccta tgctcaaaaa cacggtctgc gtagagtgct
      661 gga
//
LOCUS       DAZGBC010000591          662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZGBC010000591 DAZGBC010000000
VERSION     DAZGBC010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.595"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>662
                     /locus_tag="ACHK1J_005608"
     CDS             <1..>662
                     /locus_tag="ACHK1J_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7914700.1"
                     /translation="RMEDKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTD
                     EHGAVRAAKGLFLTADAQARAQGPVLEMAAAISQINQANSQMQALNSAAEAAGALLSD
                     INTHIRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINAGQHLDIGAMK
                     NLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVTSSEDEIIIST
                     PET"
BASE COUNT          179 a          164 c          199 g          120 t
ORIGIN      
        1 cggatggagg ataagcgcca ggaagagcat atcaagctcg caacggaata tggcaaaaca
       61 cagctgaaca tgggacacct ggtcaacagc cggcgtgaaa agcgaggtgc cggttttgaa
      121 ctgcgaacgg atgagcatgg ggctgtacgt gcagcaaaag ggctgttcct gacggcagat
      181 gcccaggcca gagcccaggg gccggtgctg gaaatggcgg ccgcgataag tcagattaac
      241 caggctaaca gccagatgca ggccctgaac agcgctgccg aagccgccgg cgccctgctc
      301 agtgatatca atactcatat taggtttgtg accgacaaaa tcagggaact tcagtctgcg
      361 gtcctgctgg gtagtgcgcc gcagggggtg gccctgacct caggtgaaca ccttcagctc
      421 tgcagtaccc gtaacaccat gatcaatgca ggccagcatc ttgatatcgg tgcgatgaaa
      481 aatctgtcgg taacggtgga aaaagccctt ggtctgtttg tgcataaaga cggggcgaag
      541 ctggtagcca accagggaga tattgagatt caggctcagc acaacacgat ggcgctgttc
      601 tctgaaaaac agctgacggt gaccagtagt gaagacgaaa ttattatcag caccccggaa
      661 ac
//
LOCUS       DAZGBC010000592          662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZGBC010000592 DAZGBC010000000
VERSION     DAZGBC010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.596"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>662
                     /locus_tag="ACHK1J_005609"
     CDS             <1..>662
                     /locus_tag="ACHK1J_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO7914701.1"
                     /translation="RMEDKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTD
                     EHGAVRAAKGLFLTADAQARAQGAVLEMAAAISQINQANSQMQALNSSAEAAGALLSD
                     INTQIRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINAGQHLDIGAMK
                     NLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVTSSEDEIIIST
                     PET"
BASE COUNT          181 a          161 c          200 g          120 t
ORIGIN      
        1 cggatggagg ataagcgcca ggaagagcat atcaagctcg caacggaata tggcaaaaca
       61 cagctgaaca tgggacacct ggtcaacagc cggcgtgaaa agcgaggtgc cggttttgaa
      121 ctgcgaacgg atgagcatgg ggctgtacgt gcagcaaaag ggctgttcct gacggcagat
      181 gcccaggcca gagcccaggg ggcggtgctg gaaatggcgg ccgcgataag tcagattaac
      241 caggctaaca gccagatgca ggccctgaac agctctgccg aagccgccgg cgccctgctc
      301 agtgatatca atacccagat taggtttgta accgacaaaa tcagggaact tcagtcagcg
      361 gttctgctgg ggagtgcgcc gcagggggtg gccctgacct caggtgaaca ccttcagctc
      421 tgcagtaccc gtaataccat gatcaatgca ggtcagcatc ttgatatcgg tgcgatgaaa
      481 aatctgtcgg taacggtgga aaaagccctt ggtctgtttg tgcataaaga cggggcgaag
      541 ctggtagcca accagggaga tattgagatt caggctcagc acaacacgat ggcgctgttc
      601 tctgaaaaac agctgacggt gaccagtagt gaagacgaaa ttattatcag caccccggaa
      661 ac
//
LOCUS       DAZGBC010000593          662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZGBC010000593 DAZGBC010000000
VERSION     DAZGBC010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.597"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..198)
                     /locus_tag="ACHK1J_005610"
     CDS             complement(<1..198)
                     /locus_tag="ACHK1J_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914702.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGIGRVASVLWPVG"
     gene            complement(240..>662)
                     /locus_tag="ACHK1J_005611"
     CDS             complement(240..>662)
                     /locus_tag="ACHK1J_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914703.1"
                     /translation="DPGAQGPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPM
                     GPPGASGDDGKSRVMGFRLGNPVTYELQEGDHGWSVNLADGSVITGLSGNTQGGVSRV
                     TTIYYRPLQMTFDGSHWRTVGVGEYVSSGAPGREYFSL"
BASE COUNT          134 a          232 c          143 g          153 t
ORIGIN      
        1 gcccactggc cacagaactg acgccaccct gccgatgccg gtaatgactc cggcactgtt
       61 ataaaccacc ttcagcgtct ctgctgaaaa taacgactga cactcatacc agtcctgacc
      121 atcctctgat ttcaggtaca tcgcacccgg cacatccgga ttttccggcg tgtattgtct
      181 gaaattcttt atgtgcatct gtaagtcctc ccggttacat gtctgaaaga tgatgcgctt
      241 tataatgaaa aatactcacg ccccggcgct cctgacgaca catactcacc aacaccaacg
      301 gtgcgccagt gagagccatc aaacgtcatc tgcagcggcc tgtagtaaat cgttgtaaca
      361 cggctgacgc cgccctgagt gttccccgaa agccctgtaa tgactgaacc atcagccaga
      421 ttaactgacc agccatggtc cccctcctgc aactcatacg ttaccggatt ccccagcctg
      481 aagcccatga cacgactctt cccgtcatct cctgatgccc ccggcggccc catcggcccg
      541 gtttctccac gctcacctct gggaccgggg tctcctttct ctcctttcgg tccctgcgct
      601 ccccgcggac cagcggggcc tgttgctccc gtttcacctt tcggaccctg tgctccgggg
      661 tc
//
LOCUS       DAZGBC010000594          662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZGBC010000594 DAZGBC010000000
VERSION     DAZGBC010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.598"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..>662
                     /locus_tag="ACHK1J_005612"
     CDS             55..>662
                     /locus_tag="ACHK1J_005612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914704.1"
                     /translation="MDWLTAKLTSDETSPQIAGYMPEGARVEWDPADSNLNATGSNKR
                     KYGLVTLKGLPEKTSSTLTPGQRYWVVVDNNNVKPAAGAGPGWWRHLLPPAKEVMVFD
                     KTVSLSSPFAIKAGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQV
                     GKNNPLWLKYAPGLALYKKDVFTETFTKDTRVTARTGILPLK"
BASE COUNT          194 a          148 c          187 g          133 t
ORIGIN      
        1 gctgaagcag aaaatcagtg ggtcgttcag gatactctaa gagcatattc tgaaatggac
       61 tggctgacgg cgaaactgac ttcagatgaa acttctccac aaattgcagg gtacatgcct
      121 gaaggggcca gagtggagtg ggacccggca gacagcaatc ttaacgctac aggaagtaac
      181 aagagaaaat acggtcttgt gacgctaaag ggtttaccag aaaaaaccag cagtactctc
      241 actcccggtc agcgctactg ggttgttgtg gataacaaca atgttaagcc agcggcgggg
      301 gccgggcccg gctggtggcg acacttgctg cctcccgcaa aggaagtcat ggtattcgat
      361 aaaacagtaa gcctcagttc tccgtttgcc attaaggccg gtgacccgat tgggcacatg
      421 ggctactatc aggcaccaaa ggatgggggg tatgaggcac gatatcaggt acatatcgaa
      481 tgcacgagta tggatgacaa tctggagaaa ttcctgacca atcctgagca ggtgggaaaa
      541 aataatccac tctggctgaa gtacgccccg gggcttgcgt tgtacaagaa agatgttttt
      601 acagaaacat tcacgaagga cacgagagtg acggccagga cgggaattct gccactgaaa
      661 ca
//
LOCUS       DAZGBC010000595          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZGBC010000595 DAZGBC010000000
VERSION     DAZGBC010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.599"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>657)
                     /locus_tag="ACHK1J_005613"
     CDS             complement(<1..>657)
                     /locus_tag="ACHK1J_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO7914705.1"
                     /translation="VTAGNGATVAPTVITEPDGTVEISVTSQTAGISTVTASINSSSL
                     SRDVMFIADIRTAQIADLVVIKDGSEADGATANTLRARVTDAFGNTLAGQTVSVMADN
                     GATVASTVTTGQDGTVEISVTSQTAGTSVVTTSINSSNASRNVTFIADVRTAQIADLV
                     VIKDGSEADGATANTLRARVTDAFGNALAGQTVSVSADNSAMVTPTVTTQPDGTVEIS
                     V"
BASE COUNT           99 a          238 c          174 g          146 t
ORIGIN      
        1 gacactgatc tccaccgtgc catccggctg cgtggtcacc gtcggagtga ccattgcgct
       61 gttgtctgcc gatacgctga ccgtctgccc ggcaagcgca ttgccgaacg catcggtcac
      121 cctcgcccgc agcgtattcg ccgtcgcacc gtccgcctct gaaccatcct taatgaccac
      181 cagatccgcg atctgcgccg tcctgacatc cgcaataaac gtcacgttcc tgctcgcgtt
      241 gctgctgttg atgctggtgg tgaccacact ggtgcccgcc gtctggctgg tgactgagat
      301 ctccaccgtg ccgtcctgcc ctgtggtgac cgtcgaagcg accgttgcgc cgttgtccgc
      361 catcacgctg accgtctgcc cggcaagcgt attaccgaac gcatcggtca ccctcgcccg
      421 cagcgtgttc gccgtcgcac cgtccgcctc tgaaccatcc ttaatgacca ccagatccgc
      481 gatctgcgcc gtcctgatat cggcgataaa catcacgtcc cggctcaggc tgctgctgtt
      541 gatgctggca gtgactgtac tgatccccgc cgtctggctg gtgacactga tttccaccgt
      601 gccgtccggc tctgtaatca ccgtcggggc aaccgttgcg ccgttgcctg ctgtcac
//
LOCUS       DAZGBC010000596          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZGBC010000596 DAZGBC010000000
VERSION     DAZGBC010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.600"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>656
                     /locus_tag="ACHK1J_005614"
     CDS             <1..>656
                     /locus_tag="ACHK1J_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO7914706.1"
                     /translation="NRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPDT
                     DTEAMPESTVSIFPGTEREQHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGAS
                     SAENALRPEIQPDMYGGKPVFSGDVMEDDAAGAVLASVMTGDTAPGTTDALPASVTHF
                     AVRRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFLA
                     G"
BASE COUNT          144 a          160 c          212 g          140 t
ORIGIN      
        1 aaccgtcgtc atcattttgg tgagccggaa ccggtggtgg tgcctccttc agtccgtggc
       61 agcagtacgg tacaacccgg ggcaccggta tccgggatcc ggtctgaagt accggagtgt
      121 caaccggata cggatacaga ggcgatgccg gaaagcacag tctccatatt ccccggtact
      181 gagcgcgagc aacatggtat tacccggcca ctgaaagccg tttctgatac tgacacagag
      241 cgcactgaaa aatgcagtcg accttctcag gaagagagcg caggcagggg ggacagtggg
      301 gcgtcttctg ctgaaaatgc cctgcgtcct gagatacagc cggatatgta cggtggtaag
      361 cctgtcttca gtggtgatgt tatggaagat gatgctgccg gggcagtact ggcatcagtt
      421 atgacaggag atactgcccc gggaaccact gatgcacttc ccgcgtcggt gacgcacttt
      481 gctgtgaggc gggatgatat cgaacatctg cagaatgaag cgttccgtct ggcctgggaa
      541 ctggctgaat cagccggttg tgctgaagag attgccatgg acagggaaag cgacctgtct
      601 gccggtttcg gggcagcagt ggagaaatgt tctcctgtag tggcgtttct ggcggg
//
LOCUS       DAZGBC010000597          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZGBC010000597 DAZGBC010000000
VERSION     DAZGBC010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.601"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>656
                     /locus_tag="ACHK1J_005615"
     CDS             <1..>656
                     /locus_tag="ACHK1J_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO7914707.1"
                     /translation="NRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPDT
                     DTEAMPESTVFIFPGTEREQHGITRPLKAVSDTDTERTEKCSRPSQEESAGMGDCGAS
                     SAENALRPEIQPDMYGGKPVFSGDVMEDGAAGAVLASVMAGDTAPGTTDALPASVTHF
                     AVGRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFLA
                     G"
BASE COUNT          140 a          159 c          214 g          143 t
ORIGIN      
        1 aaccgtcgtc atcattttgg tgagccggaa ccggtggtgg tgcctccttc agtccgtggc
       61 agcagtacgg tacaacccgg ggcaccggta tccgggatcc ggtctgaagt accggagtgt
      121 caaccggata cggatacaga ggcgatgccg gaaagcacag tcttcatatt ccccggtact
      181 gagcgcgagc aacatggtat tacccggcca ctgaaagccg tttctgatac tgacacagag
      241 cgcactgaaa aatgcagtcg accttctcag gaagagagcg caggcatggg ggactgtggg
      301 gcgtcttctg ctgaaaatgc cctgcgtcct gagatacagc cggatatgta cggtggtaag
      361 cctgtcttca gtggtgatgt tatggaagat ggtgctgccg gggcagtact ggcatcagtt
      421 atggcaggag atactgcccc gggaaccact gatgcacttc ccgcgtcggt gacgcacttt
      481 gctgtggggc gggatgatat cgaacatctg cagaatgaag cgttccgtct ggcctgggaa
      541 ctggctgaat cagccggttg tgctgaagag attgccatgg acagggaaag cgacctgtct
      601 gccggtttcg gggcagcagt ggagaaatgt tctcctgtag tggcgtttct ggcggg
//
LOCUS       DAZGBC010000598          655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZGBC010000598 DAZGBC010000000
VERSION     DAZGBC010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.602"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..552
                     /locus_tag="ACHK1J_005616"
     CDS             <1..552
                     /locus_tag="ACHK1J_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO7914708.1"
                     /translation="DIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETI
                     EKHLAWLDGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVF
                     QVFHYGLISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRM
                     GEPDPDVMSGKKRSVYSPPVKAG"
BASE COUNT          171 a          133 c          180 g          171 t
ORIGIN      
        1 gacatcatca gtatgcccag ggctattgcg tttgcgggtc aggcaaccag agattcagcc
       61 agggataatc cttatgcaga tatgatgtta gtcaggctgg aaaatgccct gacgaaggca
      121 actgaaacaa ttgaaaagca tcttgcgtgg cttgacggga tccttaaaaa cctgccggga
      181 cagatctccc tttctgatat taaatcctct tcgccggtaa atattggcgt gtacagtagt
      241 tctccggtcg gttatcgatg tgtatggctt ctgatcggat atgatcgact ggtcatgaag
      301 gtatttcagg tatttcatta cggacttatt tcccgcacaa aacgggatga gttgttgaat
      361 cagggagggt atgcggtacg caggatttac gggattattc aaaattataa atcagtcagt
      421 gttacacgtg ctgatatttt gtccggcaca gcccgtggaa aggaggcggt taaccgcatg
      481 ggggagccgg atcctgacgt gatgagcggt aagaaacgtt ctgtttattc accaccggtt
      541 aaagcggggt gagtatgcaa ctctttctgt gtgaaaaacc ttctcaggcg aaagatatcg
      601 cccgtgaact ggcgctcagt aagcggggcg acggatacat cggagatgac aatat
//
LOCUS       DAZGBC010000599          652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZGBC010000599 DAZGBC010000000
VERSION     DAZGBC010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.603"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>652
                     /gene="ydjO"
                     /locus_tag="ACHK1J_005617"
     CDS             <1..>652
                     /gene="ydjO"
                     /locus_tag="ACHK1J_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO7914709.1"
                     /translation="ALTFFISTGQCDCDEIAESNFNYMIAYMSNINYDASKPGAPALS
                     FDTYLQDNVKYRVIINNLYGSEIRIRGINKDFIGMDVTSVFRPEKMTNLISIKNRLVI
                     HYLRTIYYEQNYIHPVGSIFAAIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGIL
                     EDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAME"
BASE COUNT          213 a          104 c          122 g          213 t
ORIGIN      
        1 ggcactcact ttctttatat caacgggtca atgtgactgc gatgaaatag cagaatcaaa
       61 ctttaattat atgattgcct atatgtctaa cattaactat gatgcctcaa agccaggagc
      121 gccagcactt agttttgata catatctgca ggacaatgtt aaatatagag taataattaa
      181 taatctttat ggtagtgaaa ttcggataag aggtataaat aaagatttta ttggaatgga
      241 tgtcacttct gtatttcgcc cagaaaaaat gaccaatttg atcagtataa aaaacaggtt
      301 ggttattcac tatttaagga ctatatacta tgaacaaaat tatatacatc ctgtcgggag
      361 catttttgct gcgattcaaa aaaatgagag tctcctaaag tttccttcga taatatcaat
      421 gcttaatatc aatcttcttt ttaatccact aaatcttccg ggaatgggaa gtggtatact
      481 tgaagatatt atgagtattc cagatagttc acttagaaaa cgtctcgggt atgaggtttt
      541 aagcttctcg ctacaggcac attcattgag ccaggaatgt attgataagt tggacatttt
      601 tttcgctgac gatctgttta aatatgagtc agtatgcatc gccgcgatgg aa
//
LOCUS       DAZGBC010000600          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZGBC010000600 DAZGBC010000000
VERSION     DAZGBC010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.604"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(79..537)
                     /gene="tnpA"
                     /locus_tag="ACHK1J_005618"
     CDS             complement(79..537)
                     /gene="tnpA"
                     /locus_tag="ACHK1J_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO7914710.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          152 a          179 c          121 g          198 t
ORIGIN      
        1 ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca cacgatctga
       61 catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat agggaatcga
      121 taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct gtatcttcgc
      181 cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc tgttcctgta
      241 tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc ctttcagata
      301 tcccataaag ccggatacgc tcattttggg cgggatctcc acaagcatat ggatatgatc
      361 tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcgcacagct ttctcaaaat
      421 actgcctatt gctctacgtt tctctctgta gaacacctgt cttcggtatt ttggcgcaaa
      481 aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt tccccattgg
      541 gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg tgttttaaca
      601 aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
//
LOCUS       DAZGBC010000601          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZGBC010000601 DAZGBC010000000
VERSION     DAZGBC010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.605"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            425..618
                     /locus_tag="ACHK1J_005619"
                     /pseudo
     CDS             425..618
                     /locus_tag="ACHK1J_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          192 a          145 c          164 g          146 t
ORIGIN      
        1 aataatgact gcctcggcat gccagttaac agccatccgc aatggcgtgt tcggttttgc
       61 gaataaaaaa tggttactgc agaagcggat tttcaccacc tgtcaaccat gaatggtcag
      121 tggagtgaga cgactggtat gatttggtcg atgacttctt ccggtgaata cggacagaaa
      181 cattgaaata ttgtaagaga gaacgttcag aattctgtgt cacgtgaaaa tactacagcg
      241 ttatcacctt attcagtcga ccttcaaaat aaacgtctaa ccgtgacaat caccatttgg
      301 attgcccact ggggaggcga acgattgcct cgacggcatt tataatgcca gcccacattg
      361 ttggcgctga caatactaag gacaaacgat gagggagaac cgtcttatac cactagcgct
      421 gacaccggca caccttcccc gtgaaataca ccggctggag cctgaagaaa gctgttgccc
      481 ggagtgtggc ggtgagctgg attatctggg gaagtcagcg ctgaacaact ggaactggtg
      541 agcagctccc tgaaagtgat cagcacagaa cgggtaaaaa aagcctgtaa aaaatgtgac
      601 ggtaccgttg aagcgccggt ctcgtaagaa taatcctgga caaataa
//
LOCUS       DAZGBC010000602          646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZGBC010000602 DAZGBC010000000
VERSION     DAZGBC010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.606"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>646)
                     /locus_tag="ACHK1J_005620"
     CDS             complement(<1..>646)
                     /locus_tag="ACHK1J_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914711.1"
                     /translation="AESRNVEREALIDSEILRGQRCAWILGTYIQSPAGNKADDLLEA
                     MEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKALKGAVNKLTTRVEGIVHPLPPAL
                     PCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMTGKGLGAFDAWLDKRWDTPGILV
                     VITLSLPASPREEDADALSMVVLSNRNTHAWPDALCLHRPERGTETMLTKTLTRA"
BASE COUNT          141 a          185 c          167 g          153 t
ORIGIN      
        1 cgcccgggta agtgtctttg tgagcattgt ttctgttccc ctttcgggtc gatgaagaca
       61 aagcgcatcc ggccaggcat gggtgttgcg attgcttaat accaccattg agagagcatc
      121 agcatcttct tcccggggag aggcaggtaa cgacagcgtg atgacaacca gaatgcccgg
      181 ggtgtcccat cgcttgtcca gccaggcatc aaacgccccc agcccttttc cggtcatcag
      241 gcgaaaaatc ctgcccgtct tgagtgagag gtcggcttta agctgctctt caatcagggg
      301 atacagatcg ttatcacaat ccagcaccag ccagcatggt agcgcgggcg gaagcggatg
      361 aactatcccc tctacacgcg tggtcagttt attcaccgcc cctttcagag ccttcgttaa
      421 atctgtctgg tattctggta atgccgcgta cctgaccggt ttatcacatc ctcttgggtg
      481 cgagaaatca atagcaggag ccgcgacttc cattgcctca agcaggtcat cggctttgtt
      541 tccggccggg ctctgaatat aagtaccgag gatccaggca cagcgctgac cacgcagaat
      601 ttcactgtca ataagcgctt cccgctcgac atttcgggac tcagcc
//
LOCUS       DAZGBC010000603          644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZGBC010000603 DAZGBC010000000
VERSION     DAZGBC010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.607"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..176)
                     /gene="subB"
                     /locus_tag="ACHK1J_005621"
     CDS             complement(<1..176)
                     /gene="subB"
                     /locus_tag="ACHK1J_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO7914712.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGK"
     gene            complement(193..>644)
                     /gene="subA"
                     /locus_tag="ACHK1J_005622"
     CDS             complement(193..>644)
                     /gene="subA"
                     /locus_tag="ACHK1J_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO7914713.1"
                     /translation="PAAYHPVSSVNKKQDPVIRVAALAQYRKGETPVLHGGGVTGSRF
                     GNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATAIVSGVLAAMVSCNPRATATELKRT
                     LLESADKYPSLADKVTEGRVLNAEKAISMFCKKNYIPVRQGRMSEEL"
BASE COUNT          157 a          183 c          123 g          181 t
ORIGIN      
        1 tgtttccctt caatacaaaa ataagggtta ttatctattt gccctgtatg aaactgatca
       61 ataacaacgc ctgaaaacat gccatcccgg gcatcgccag tccactcagc catggcagga
      121 ttaagggata aacatcccat aacacctgca catacaaaaa acctcttaat cgtcatattc
      181 acctccggaa ttttacagtt cttcactcat ccttccctga cggacaggaa tataattttt
      241 cttgcagaac atgctaattg ctttttctgc attaagaact ctcccttccg tcaccttatc
      301 tgcaagtgag gggtatttgt ctgcagactc cagcagtgta cgcttcagtt ctgtcgctgt
      361 tgcacgggga ttacatgaga ccatcgctgc cagaacgccg gaaactatag ctgttgcttc
      421 cgaggttcca ctaccgatcc ccgtttttcc atctggtctg aggaatgtaa tattctgtcc
      481 cggtgcggca atatcaaccc agccgtttcc gaaccgactt ccggtgaccc ctccaccatg
      541 caatacaggt gtttctcctt tccggtactg tgccagagct gccactctta tcacaggatc
      601 ctgcttttta ttcacactgc tgaccggatg gtaagccgca ggcc
//
LOCUS       DAZGBC010000604          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZGBC010000604 DAZGBC010000000
VERSION     DAZGBC010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.608"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(68..>640)
                     /locus_tag="ACHK1J_005623"
     CDS             complement(68..>640)
                     /locus_tag="ACHK1J_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914714.1"
                     /translation="ASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSFPYAGEWLA
                     EDEIRAVLGAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRLVVKESDHP
                     CWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDMLRIPDEPPRR
                     WFDRGVLREVVREARNEIRSMADALAKIRK"
BASE COUNT          128 a          212 c          175 g          125 t
ORIGIN      
        1 ggcgacatcc ttttgctctt tccggggacc ggctggaaaa ggcggagatt tttccccgcc
       61 agcgtcatca tttccggatt tttgccaggg catccgccat gctgcggatt tcgttccggg
      121 cttcccggac cacctcccgc agaacaccac ggtcaaacca gcggcggggc ggttcatccg
      181 gtatacgcag catatcgcag gccagcccac tggacaggat atgctccaca cactcactga
      241 ccagatacat ttccaccgac gaaaaacgag cgccccggtt caggatggca tcgagcacca
      301 cgggcaggtt ttcgtcgtct tcatcgagcc agcaggggtg atcgctttcc ttcaccacca
      361 ggcgaaagcg gcgcgtctgg cgggtcagca acgtctgacc ggttgtggtc agcgccgcac
      421 ggatacgccg cgcatcttct gccacctggt aactgacact gcatactgca tcgcgtaccg
      481 caccaagaac ggccctgatt tcgtcctctg ccagccactc accggcatac gggaaagaca
      541 cctgcgttcc gtctgccgga agtaccggca gcgactgcca ctgactgccg gacagaacaa
      601 gttccgctcc ggttccggtc agtttctgaa tggcagaggc
//
LOCUS       DAZGBC010000605          639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZGBC010000605 DAZGBC010000000
VERSION     DAZGBC010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.609"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..571
                     /locus_tag="ACHK1J_005624"
     CDS             <1..571
                     /locus_tag="ACHK1J_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO7914715.1"
                     /translation="LWHMHPIMFLASLIQPNEDIRILRLRAFLRMIRIGEGTIQEDGY
                     RTMFTGVKFTDFSKHPNIRHEANGVVSTAAGAYQFLYGTWRNLQRRYSFPDFSPSNQD
                     LGCIALIAGRKALDVVMQGKISEAIHLCRIEWASLPGSPHGQPTANKKMIMEKYELYL
                     AEEKEGKTSLHATTEKIVKFIEGNYPEYL"
BASE COUNT          212 a          113 c          124 g          190 t
ORIGIN      
        1 gttgtggcat atgcatccaa taatgttttt agcctcccta attcaaccta atgaagatat
       61 tagaattctt cgtttacgtg catttctacg aatgatccgc atcggtgaag ggacaattca
      121 ggaagacgga tacagaacga tgtttactgg tgtaaaattt acagatttta gtaaacaccc
      181 caatatcagg catgaagcta atggtgttgt ttcgactgct gctggagctt atcaattttt
      241 atacggaaca tggagaaatt tgcaacgtag atattcattt ccagatttca gtccgagcaa
      301 tcaagatctc ggttgtatag cgctcattgc aggtcgaaaa gcattagatg tagtaatgca
      361 gggtaagatt agtgaagcca tacatttatg tcgtattgag tgggccagtt tacccggttc
      421 accgcatggt caaccaactg caaataaaaa aatgattatg gaaaaatatg aactctactt
      481 agctgaagaa aaagaaggta aaacaagttt acatgccacc acagaaaaaa tagtaaaatt
      541 cattgaaggt aattacccag agtacctatg aaaaaattca ttttcctgtt actaattcca
      601 gtgttttgta atgcaaacac tcctactgtt ttcaagtct
//
LOCUS       DAZGBC010000606          636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZGBC010000606 DAZGBC010000000
VERSION     DAZGBC010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.610"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>636
                     /locus_tag="ACHK1J_005625"
     CDS             <1..>636
                     /locus_tag="ACHK1J_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7914716.1"
                     /translation="LQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDV
                     PRAIPWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIM
                     TIVATTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPV
                     PLYPPLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHT"
BASE COUNT          135 a          202 c          172 g          127 t
ORIGIN      
        1 tctgcagtta ttcaccacac agcctgaaac acacggcagc ctgataaagg ccggtgacct
       61 ctggcacggt atggtcgcca ttaccctgcc cccccagaat ctgcggacat tcaaccagct
      121 ggtcagggat gtgccccgcg caatcccctg gcgtatccgg atggatctga tgcccggcgg
      181 catgaaggcg cttggggtca agaaaaccct gctgacctac agcagcttta tttcccctct
      241 ccggcccatg tatgactcgg tgatgatgct gaatgacatc aacaaacatg atccggtctg
      301 tatcatgacc attgttgcca ccacatgggg aaacagccgg gaagtctgta cccgcaacca
      361 ggcgctcctg aaatctgcgc tggaaggctg gggagtatgc ggcaccacca ccaccttcgg
      421 tgatccgaga cgtgcctggg tgaacacggt tctggcggcc tcccacggtt ctggtccagt
      481 ccccctttac ccgccccttt cacacgccct ttccctgttt cccctgaaca gagccggctc
      541 tgtctggcgg ggacagggaa atctgatgat gcacaccgag gacgggtctg catgggaggt
      601 cgcgctggca tcgtcacaac agaacaagca cacgga
//
LOCUS       DAZGBC010000607          635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZGBC010000607 DAZGBC010000000
VERSION     DAZGBC010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.611"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..477
                     /gene="espX1"
                     /locus_tag="ACHK1J_005626"
     CDS             <1..477
                     /gene="espX1"
                     /locus_tag="ACHK1J_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO7914717.1"
                     /translation="EKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSN
                     IFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGFRYTGVFKTLASIDDKPSRFEILI
                     PLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          244 a          115 c           97 g          179 t
ORIGIN      
        1 gaaaagagca acctttcata tgggtattat tttggttgtg tattatccaa tatatcatgt
       61 tttgaaagcg acttatcaaa caccatcttt tcaaatggag aaataaacaa cctcttcatt
      121 aaaaaaagca acatatttgg aacatcgttc actaatacca tgataaaaaa tttgcgctgt
      181 gaagatatta tgcctggccg atggaccact caacttgtca ataagcactt aggtttcaga
      241 tacacaggag tattcaaaac cctggcaagt attgatgaca aaccgtcccg tttcgaaatt
      301 ttgataccat tggtacaaac tttagttcgc gacaatgtaa agctaaataa tgatgtgtat
      361 aaagaactga aaaaatttat gcacgattac gacaaaacat ccccagaaat gagaaaatac
      421 cttcagagca tcaacgagtc tatgttcctt atgaaaaaaa tatctcatca agattaaaac
      481 accccaaaaa agaagtaatt tcagaatgaa attacctgtg ataattatta attaactgca
      541 gagaaataaa aaataatctg aagcatgctc tgaaacatcc tactctgtaa ctctaatgat
      601 ggcaccttgc tcttcttata aagaagggaa caatt
//
LOCUS       DAZGBC010000608          635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZGBC010000608 DAZGBC010000000
VERSION     DAZGBC010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.612"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            144..400
                     /locus_tag="ACHK1J_005627"
                     /pseudo
     CDS             144..400
                     /locus_tag="ACHK1J_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          197 a          127 c          149 g          162 t
ORIGIN      
        1 gaagtaaatt aatttgaagt aaataaagca aaatatcaga agaatatatc ggggcgcaca
       61 gtctatctct gcctttgctt catgggatgt tctttgtata ttttacttgc tgtttaagta
      121 tgtatataag gagtgcattc attatgccaa tgaagcttgt aaagaagaat ccccggagca
      181 aagtgattcg ggagcttagc tgtgagcaag agatggcagt aaaggcggtc tgcggactga
      241 aaaaacagca actacggcac tacatgccct ggccaatgat cttctccggg aaatgcgtga
      301 gtatgatgca tggttacagt gtgactgtat tccgggagat tcgccgacca tgaattttgc
      361 tgctcttaaa aacaacacgg gaacttttta tctgtccagt ttggatatag ataattattg
      421 tactaataag ggaagccaac accagccgac agaccagaga cgccgctgct cattcagtga
      481 aacgcagata aacagtaaag caaatccaga ctccagtacg gaataatcgt cagcggtatt
      541 aacgggatcc ggtgctccca gtcaaacacc aggctgctga tgttttctct cccggcggta
      601 aactgattcg tcatgccata agtaagaaag aagaa
//
LOCUS       DAZGBC010000609          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZGBC010000609 DAZGBC010000000
VERSION     DAZGBC010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.613"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>628
                     /gene="eilA"
                     /locus_tag="ACHK1J_005628"
     CDS             <1..>628
                     /gene="eilA"
                     /locus_tag="ACHK1J_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFO7914718.1"
                     /translation="KNNTQQYIFGDFVLKNNGILLFKNKEYHIPPKELGVIILLLNAD
                     GEIVSKEEIIDKVWSASVASDESLTRCIYALRKLLHENKQCKYIETVYGRGYRFTVPI
                     VIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYELVQGLSKYAFCGLDILPASVTNEA
                     SDFSTIHQFINKTGPEYYIMGQVVHYGKNWRLFIELIHARTHKLIDHQ"
BASE COUNT          200 a          111 c          117 g          200 t
ORIGIN      
        1 gaaaaaataa tacacaacaa tatatatttg gtgactttgt attaaaaaac aatggaatat
       61 tattattcaa aaacaaagaa tatcatattc ccccgaaaga acttggcgtt atcattctat
      121 tacttaatgc tgacggtgaa attgttagta aagaagagat tatcgataag gtctggagcg
      181 cgagtgttgc cagtgatgaa tctttaacac ggtgtattta tgcattgcgt aaacttttgc
      241 atgagaataa acagtgtaag tatatcgaaa cggtctatgg tcgtggatac cgttttactg
      301 tccccattgt gattgtaacc gataatgaac cggtaaagtc gaccaccact cttgccgttt
      361 ttccctttcg tactgaaggt tcgatcaatg ttctaaaact ccattacgaa ctggtccagg
      421 gcctttcaaa atatgctttt tgtggtttag atattttacc cgcttcggta acgaatgaag
      481 ccagtgattt ctccactatc catcagttta tcaataaaac aggcccagaa tattatatta
      541 tgggtcaggt cgttcactat ggtaaaaact ggcgtttatt tattgaatta atccatgcca
      601 gaacgcataa attgattgat catcaaag
//
LOCUS       DAZGBC010000610          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZGBC010000610 DAZGBC010000000
VERSION     DAZGBC010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.614"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..414
                     /gene="traF"
                     /locus_tag="ACHK1J_005629"
     CDS             <1..414
                     /gene="traF"
                     /locus_tag="ACHK1J_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO7914719.1"
                     /translation="LDDNWTLGLVAQNLLPRSIETKEVNGVTETFRIRPQVTAGVSWH
                     NDMFTTALDVDLTPASGFTSDSKRQFAAIGAEFNAWKWAQLRAGYRQNLASNDGSAFT
                     AGLGISPFDVVHIDVAGIAGTDNNYGAVAQLQFTF"
     gene            complement(404..>625)
                     /locus_tag="ACHK1J_005630"
     CDS             complement(404..>625)
                     /locus_tag="ACHK1J_005630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914720.1"
                     /translation="SCRIPERFHHKGFVFDMRFIFVKHAFPHAKMICQITARSEAALQ
                     VWLCRLMVGKREDAGLDTPVSTGAEMIRM"
BASE COUNT          155 a          169 c          155 g          146 t
ORIGIN      
        1 ctggatgaca actggacact gggtctggtc gcacagaacc tgctcccccg cagtattgag
       61 acaaaagaag tgaacggtgt cactgaaact ttcaggatcc gcccgcaggt gactgccggt
      121 gtctcctggc acaacgacat gttcactaca gcactggatg ttgacctgac cccggccagt
      181 ggcttcacat cagacagtaa acgtcagttt gctgccattg gtgctgaatt taatgcctgg
      241 aaatgggcac agttacgtgc aggctaccgc cagaatctgg ccagcaatga tggcagtgcg
      301 ttcaccgccg ggctgggtat ctcaccattt gatgtcgtcc atattgatgt ggcgggtata
      361 gcaggcacag acaacaacta cggtgccgtt gcacaactgc agttcacatt ctgatcatct
      421 ctgccccggt actgaccggg gtatccagac ctgcatcctc gcgttttccg accatcaaac
      481 gacacagcca gacctgtagt gctgcctctg atctggctgt tatctgacat atcattttcg
      541 catgaggaaa tgcatgttta acaaaaataa aacgcatatc gaaaacaaag cctttatggt
      601 ggaaccgttc cgggatccga cagct
//
LOCUS       DAZGBC010000611          622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZGBC010000611 DAZGBC010000000
VERSION     DAZGBC010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.615"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>622
                     /locus_tag="ACHK1J_005631"
     CDS             <1..>622
                     /locus_tag="ACHK1J_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO7914721.1"
                     /translation="QLMTPVNDALYRYVMNTRKVHTDDTPVKVLAPGQKKAKTGRIWT
                     YVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAFTGYDRLFSAEREG
                     GALTEVACWAHARRKIHDVYISSKSAKAEEALKRISELYAIEDEIRGLPESERLSVRQ
                     QRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSD"
BASE COUNT          165 a          148 c          186 g          123 t
ORIGIN      
        1 gccagttaat gacaccggtg aatgatgccc tgtaccgtta tgtaatgaac acccgcaagg
       61 ttcacactga tgacacaccg gtaaaggtac tggcaccggg tcagaaaaag gcgaaaacag
      121 ggcgtatctg gacgtatgtc cgggatgatc gcaatgtggg ttcgtcatct cctccagcgg
      181 tctggttcgc gtactcgccg aaccggcagg ggaaacaccc ggagcaacac ctccgcccct
      241 tccggggtat cctgcaggcg gatgcgttca caggttacga caggttgttc agtgcagaac
      301 gtgaaggtgg tgcactgaca gaagttgcgt gctgggccca tgcccggcga aaaatccacg
      361 atgtatacat cagcagcaaa agtgcgaagg cagaagaagc cctgaagcga atcagtgaac
      421 tgtacgccat cgaggatgaa atacggggat taccggagtc agagcgtctt tccgtcaggc
      481 agcagcgaag caaagtgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggta
      541 cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat cagtgggatg
      601 ccctctgtta ttacagtgat ga
//
LOCUS       DAZGBC010000612          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZGBC010000612 DAZGBC010000000
VERSION     DAZGBC010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.616"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..342
                     /locus_tag="ACHK1J_005632"
     CDS             <1..342
                     /locus_tag="ACHK1J_005632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914722.1"
                     /translation="QHDHIWYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRP
                     LINESIWNGQPDGLACSISHFMKFIDNPKKLNLIIDIDQRVANTSDMIDSLLLLAECG
                     MDVIYAPHTLT"
     gene            320..523
                     /locus_tag="ACHK1J_005633"
     CDS             320..523
                     /locus_tag="ACHK1J_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFO7914723.1"
                     /translation="MPHILLPELIPEAAKIVPVFEGEKQKGTIVVSTEDVFDGNNKEH
                     IGKANDIEIRLLDLGLLPLLTEL"
BASE COUNT          208 a          116 c          119 g          178 t
ORIGIN      
        1 cagcacgatc acatatggta cttaactggc tactcaagaa aggacgcttc aaagcatgtc
       61 gtgtttagtg ataatgaacc gaaccaaaat acaatagata cctttgagcg gcattataaa
      121 aagaatcgtc ctttaattaa tgaatctata tggaatggac aacccgatgg gctggcttgc
      181 tcaatatcac attttatgaa atttattgat aaccctaaaa aactaaatct tatcatagat
      241 attgatcaga gagttgccaa tacttcggat atgattgatt ccttgcttct gttggctgaa
      301 tgtgggatgg atgttatata tgccccacat actcttacct gaattaatcc ctgaagccgc
      361 aaaaatagta ccggttttcg agggcgagaa gcaaaaggga actattgttg tttcaaccga
      421 agacgtattt gacggaaata ataaggaaca catcggaaaa gctaatgata ttgaaataag
      481 acttctggat cttggactgc tacctttgct aacagagctt taatccaacc ttatcactca
      541 tgggtagatg acgggacagt tacacccgtt aatggcctta tttttaacac atcagataaa
      601 attgaatgct caccattaag a
//
LOCUS       DAZGBC010000613          620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZGBC010000613 DAZGBC010000000
VERSION     DAZGBC010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.617"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..180
                     /locus_tag="ACHK1J_005634"
     CDS             <1..180
                     /locus_tag="ACHK1J_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7914724.1"
                     /translation="SYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGVDLPNEAPGFSLT
                     DITQESKISSKSFND"
     gene            196..>620
                     /locus_tag="ACHK1J_005635"
     CDS             196..>620
                     /locus_tag="ACHK1J_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914725.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHL"
BASE COUNT          155 a          140 c          167 g          158 t
ORIGIN      
        1 tcttacctga gactgagtaa gaacgggata gagcacggct catcgggtga gtttattgtg
       61 aagacgtctg actatctggt gcctggttct ggcgtagatc tccccaacga agcacctggt
      121 ttcagcctga cggatatcac acaggaaagc aaaatcagca gcaagtcttt taacgactga
      181 cagggagtat gaatcgtgtc tgtattaccc aaaatcgtct ggccgatccc ctccagccgt
      241 cggggaagtg agttcaaaaa tcaggaagag atcctctctc atttgggggg agaatctacc
      301 gggctgtaca tgataggccg tagtggcatg tggcatggtg ggatccatat caccgaagcc
      361 acgacaccat ggtgtgcgct tagcggtaag tctccacttg aagcgataga ttttccggtt
      421 ccttttaaag gtgagcaggc aatccgatgc atggctgacg gagaagtgat tgcttaccgc
      481 atctgcaggg attatctgac gattgcatgg gaatccggtc cgctgaattt ttccggctca
      541 tttgttctgg tgaaacacta tatccagccg ggtgagaaag aaaccagtgg actgcatttc
      601 tatactctgt atatgcatct
//
LOCUS       DAZGBC010000614          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZGBC010000614 DAZGBC010000000
VERSION     DAZGBC010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.618"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>619
                     /locus_tag="ACHK1J_005636"
     CDS             2..>619
                     /locus_tag="ACHK1J_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914726.1"
                     /translation="MYNRTTDTDNVKTPDTPMWPCDNREEQQPINSTFSGEGYNPEQY
                     DLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESISSTPLTRQAQEILNQIN
                     NIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSVQTMFSDTNFIAEQRAD
                     LPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNMTGQN"
BASE COUNT          237 a          128 c          106 g          148 t
ORIGIN      
        1 tatttataac cgcacaacag ataccgacaa tgttaaaaca ccagacacac cgatgtggcc
       61 ttgtgataat cgggaagaac aacaacccat taattccaca ttctctggag aaggatacaa
      121 ccctgaacaa tatgatttag cgcggcaaca cctcccacaa atttatgcgt gccgcacgaa
      181 caccaaatat acagatgcag actactcaaa agttgtggct caacttgtaa gtcttattac
      241 taacattgaa tcgataagtt cgacaccact tacgcggcag gcacaggaaa tacttaatca
      301 aattaataac attagatacg aaaagaacaa aagtgctgaa tgtcgaatca ttgttgtcgc
      361 taatcctaaa cccgacaagg caatcataac taaaattagc gtagaagagg gaataccagt
      421 aagattcagt gtccaaacca tgttttcaga tactaatttt attgctgaac aacgagctga
      481 cttaccgact aatataaaag atattcaaag cctgtatcaa aaaatgacga agttatatat
      541 tgagcacagt gaaaataaaa acaggatgaa agtctttgcc ggaaccaact ttattgattt
      601 caatatgaca ggacagaac
//
LOCUS       DAZGBC010000615          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZGBC010000615 DAZGBC010000000
VERSION     DAZGBC010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.619"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..616
                     /locus_tag="ACHK1J_005637"
                     /pseudo
     CDS             1..616
                     /locus_tag="ACHK1J_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695252.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          202 a          109 c          115 g          190 t
ORIGIN      
        1 aattagagtt tatatcgact aatattgcag agtttctaga caactttacg aaaattacag
       61 attccagact ttgcaaaggt ttcagtgact gggctaattc agtcaaagaa ggcgcatcga
      121 atgacttcaa aaaaaatgtg gatatagcat tggtgagatt gtttaaatgt gttgaactcc
      181 acaatagtaa acttgactta acagatcttc acttggggtc tttgccacct cttccatcct
      241 ggattgaggt gctttatctt cgtcataatg gccttgccac aatacaggct cccaaattct
      301 gcaaagaatt agagctcgat ttcaataatt atatggtatt tccaaaagta tccgatggaa
      361 ttacccaggt ctccgtagat aataatctga tatcgcgtgt tgactcatct ccgtcaaagg
      421 ctatgacaat ttctatttat cgcaataaaa tatggtaaat accagcaatg cctgagacta
      481 ctaagctgtt taattgtggc gataataaga taaaagaaat tcaatacttc ccaaatcatc
      541 tggagaaagc acgtatggag tgtgatattg aggttgtctc tgcgatatat agcaataaag
      601 aattactttt taaaga
//
LOCUS       DAZGBC010000616          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZGBC010000616 DAZGBC010000000
VERSION     DAZGBC010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.620"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>616
                     /locus_tag="ACHK1J_005638"
     CDS             <1..>616
                     /locus_tag="ACHK1J_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFO7914727.1"
                     /translation="WDKNRQLTTDEISCYLKKIAANAKNTQVNYPTDFYLPNSNSTYL
                     EVALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRSL
                     GHEKENSLKRIINSFNSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGA
                     FVCMAAQEVIEQMESNSDSAPYTLLKNYADRFKKYSAEEQYEIDF"
BASE COUNT          233 a          126 c           98 g          159 t
ORIGIN      
        1 tgggataaaa atcgtcaatt aaccacagat gaaatatctt gctatcttaa aaagatcgcc
       61 gccaatgcaa aaaatactca agtcaattat cctactgatt tctacctccc caattccaac
      121 agcacttacc tggaagtcgc tctcaatgat aatattaaga gcgatccatc atggccgaaa
      181 gaagtccagt tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa
      241 atagttaatg aaaaaaacaa cacacaacaa ataaaatgca tcatattcaa ttcattacgt
      301 tcactaggcc atgaaaaaga aaattcactt aagcgtatca ttaacagttt caatagtttc
      361 aattgtgacc ccacgagaga aacgccgaat aataagaata taacagatca tttaactgaa
      421 ccagagataa tatttttaca tgccgatctt cagcaatact taagccaaag ttgcggtgca
      481 tttgtgtgca tggcagccca ggaagtgatt gaacaaatgg aaagcaattc tgacagcgcc
      541 ccctatacgt tattaaaaaa ctatgctgac agatttaaaa aatattcagc agaagagcag
      601 tacgaaattg attttc
//
LOCUS       DAZGBC010000617          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZGBC010000617 DAZGBC010000000
VERSION     DAZGBC010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.621"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..>615
                     /locus_tag="ACHK1J_005639"
     CDS             31..>615
                     /locus_tag="ACHK1J_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO7914728.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDVNKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQ"
BASE COUNT          210 a          100 c          126 g          179 t
ORIGIN      
        1 gtgataaata tatttaatga tgaggcattt atgaaaaaat attatccact attaactgta
       61 attcttcttg gtttaccttt gtgctctcag gcattaactc ttaatctacg tggaggatac
      121 agaagcgcca gtcacgctta tgaaacgttg attcaggtta gtcaggggtg gaataatggt
      181 tggtgggcaa gttttgagtc aaacacctgg aatacaattc acaacaatga aaatgaattg
      241 tttgctctca attataataa aatagatgta aataaaacat ataaaataga tgacaaatgg
      301 gctctaatcc cagggattca gacacaatgg atgtcagcag gaagtcagta taacccttat
      361 ctgaaaattt cgtatcaggc aacgccagat cttaatctgg ccttacgcta tcgctatgac
      421 tggaaagctt tccgacagac ggatttagat ggcaacaggg cgcgtaatga tcagcatcag
      481 tttgatggtt atgtgacata caaaattaat gattcatggt tgtttgcatg gcagaccacg
      541 gtatatacga aggttaataa tttcaaatat gggaatcata aaaaaacagc aactgaaaat
      601 gcatttgttc tgcaa
//
LOCUS       DAZGBC010000618          607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZGBC010000618 DAZGBC010000000
VERSION     DAZGBC010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.622"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..351)
                     /locus_tag="ACHK1J_005640"
     CDS             complement(<1..351)
                     /locus_tag="ACHK1J_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HFO7914729.1"
                     /translation="MVYTIDKLHLGQKASFEKTITEADVLIFSGISGDINSVHINEEA
                     AKKSIFKKRVAHGVLVSSLTSAVLGMKLPGEGTIYLRQENNFRKPVFIGDTVKATCEV
                     VDINKEKNIVKLSTI"
     gene            complement(354..>607)
                     /locus_tag="ACHK1J_005641"
     CDS             complement(354..>607)
                     /locus_tag="ACHK1J_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HFO7914730.1"
                     /translation="KVQQITFSSDTARKNNKEVFYITERAVFRLSQKSIELIEIAPGV
                     DLQKNIIECMEFVPEISPSLKIMDERIFLEQPMGLELKD"
BASE COUNT          192 a          118 c           88 g          209 t
ORIGIN      
        1 gattgttgaa agctttacaa tatttttttc tttattaata tcaactacct cgcaagttgc
       61 tttaacagtg tcaccaataa aaacaggttt gcgaaaattg ttttcctgac gaagatatat
      121 tgtgccttca cctgggagtt tcatacctaa tactgctgat gttagagatg atacaagaac
      181 accatgagct actcgtttct tgaaaatact cttctttgca gcttcctcat taatatgaac
      241 agaattaata tcgccagaaa taccagaaaa aattaaaaca tctgcctctg ttattgtttt
      301 ttcaaagctc gccttctgac ctaagtgtag tttatcaata gtgtatacca tgattaatcc
      361 tttagttcta atcccatcgg ttgttccaaa aaaatacgct catccataat tttcaaagac
      421 ggagaaattt ctggaacaaa ctccatgcat tcaataatat ttttttgcaa atcgactccg
      481 ggcgctattt caatcaactc aatggatttt tgagaaagtc taaaaacagc acgctctgta
      541 atataaaaca cttctttatt atttttcctt gcagtatctg acgagaaagt gatttgttga
      601 accttct
//
LOCUS       DAZGBC010000619          604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZGBC010000619 DAZGBC010000000
VERSION     DAZGBC010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.623"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>604
                     /locus_tag="ACHK1J_005642"
     CDS             <1..>604
                     /locus_tag="ACHK1J_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO7914731.1"
                     /translation="NQDSDTLAALWPLYAKNNAQLMRDATADSLRQQLNAFVQLPPAS
                     DARTQGTQHAYDILKSYLMLARPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLL
                     GFYAQNLPAHPEWKIKPDDELISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLT
                     LADMTGDTDASTVFSTDEVVPGMFTRQAWEEQVQDAINEVV"
BASE COUNT          158 a          155 c          171 g          120 t
ORIGIN      
        1 aatcaggaca gcgacacgct ggccgcgctc tggccgctgt atgcgaaaaa taacgcacag
       61 ctgatgaggg atgccacagc agacagcctc aggcagcagc tgaatgcctt tgtgcaactc
      121 cctcctgcca gtgatgcccg tacgcagggc acacagcatg cttacgacat actcaaaagt
      181 tatctgatgc tggcgcgccc ggacaaagcg gacgccggct ggctggcaaa aaatgcgctg
      241 aaagccttac cgaaacgccg gaatgtgccg gatggcatat ggcagaacgt cgcaccgaaa
      301 ctgctggggt tttatgcgca aaaccttccg gctcatccgg aatggaaaat taagcctgac
      361 gatgaattaa tcagtacggt ccgtcagatt ctgcttaagc aaattggcca gcggaatgct
      421 gagtccgggt tatatcagga gatgcttaag cgcgtggcca gtaactggcc tgatttaaca
      481 cttgcggata tgaccggtga caccgatgcc tcaacggtat tcagcactga cgaagtcgtt
      541 ccgggaatgt ttacgcgtca ggcctgggaa gagcaggtac aggatgcgat taacgaagtt
      601 gtca
//
LOCUS       DAZGBC010000620          599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZGBC010000620 DAZGBC010000000
VERSION     DAZGBC010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.624"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHK1J_005643"
     CDS             complement(<1..186)
                     /locus_tag="ACHK1J_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914732.1"
                     /translation="MGRKLIWRAVASAPGHEEVHVFVDASDRAEAKKHLYQRLAEEWR
                     CSVNRITFHNMWNENELR"
     gene            complement(171..>599)
                     /locus_tag="ACHK1J_005644"
     CDS             complement(171..>599)
                     /locus_tag="ACHK1J_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO7914733.1"
                     /translation="PVLPDVREFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTA
                     LARQTLAELTGIINAGRYSKVLPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSA
                     RCSDSALVTMHSLACELLPEWREFFDALLENGGKSLWAEN"
BASE COUNT          137 a          174 c          146 g          142 t
ORIGIN      
        1 ccgtaattcg ttttcattcc acatattgtg gaaagttatc cggttcactg aacaacgcca
       61 ttcctccgcc agacgctgat acaaatgctt tttcgcttct gcgcggtcag atgcatcaac
      121 aaacacatgc acttcctcgt gtccgggtgc tgaagccacg gcacgccata tcagttttct
      181 gcccacaggc ttttccctcc gttttccagc agagcatcaa aaaactcacg ccactccggc
      241 aacagttcac aggccagact gtgcatggtg accagcgcag aatcgctgca acgggcagac
      301 actttttcca gacgatgcac cggctgaccg gctgcgtgcc cctgtttata aatattcagg
      361 tcgtaaatct gtgttgaaag aagggtgacc gcaccttcag gcagcacttt gctgtagcga
      421 ccggcgttga taatgcctgt cagctctgcc agtgtctgtc tggccagcgc cgtataatcc
      481 atgcagttaa ccagggtgcg gactttcggc aggggaatac ccagttcacg gtagggcata
      541 aggttttcca tgacattgac cgtgccgcgg atgaactcac gcacatccgg cagaaccgg
//
LOCUS       DAZGBC010000621          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZGBC010000621 DAZGBC010000000
VERSION     DAZGBC010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.625"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>598
                     /locus_tag="ACHK1J_005645"
     CDS             <1..>598
                     /locus_tag="ACHK1J_005645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914734.1"
                     /translation="YSGMINTLSFDFDSIQYGYFESEKLPCRMSVIVGRNGCGKSTTL
                     ARLSRVAYSSTQDRKKEQIAKIGEILPEGLGFPKIINLSYSAFDSFQIPGCTFKEKKQ
                     LRQDILDGKGRYIYCGVRDVGAELDYVLANVDENNMDIEFITLDRQERTILKPLEVLS
                     EEFYGVLIKIHKDSDKWNLFNKVMMKLREEVSLSEIID"
BASE COUNT          210 a           67 c          135 g          186 t
ORIGIN      
        1 tgtattcagg aatgattaat actctttcgt ttgatttcga ttcaatacaa tatggatatt
       61 ttgaaagtga aaaactacca tgtagaatgt ccgtaattgt aggccggaac ggttgtggga
      121 agagtacaac attggcaaga ctttctaggg ttgcttattc ttcaactcag gacagaaaaa
      181 aagaacagat agccaaaatt ggtgaaattc ttccagaggg attaggtttt ccgaaaataa
      241 tcaacctttc atacagtgct tttgatagtt ttcaaatacc cgggtgtact tttaaagaaa
      301 agaaacagct aaggcaagac atacttgatg ggaaaggtag gtatatatat tgcggagtca
      361 gggatgttgg tgctgaattg gattatgtat tggctaatgt agatgaaaac aatatggata
      421 tagagtttat tacattagat aggcaagaga gaaccatatt aaaacctctt gaagtactta
      481 gtgaagagtt ttatggtgtc ttgataaaaa tacataaaga tagtgataag tggaatttgt
      541 ttaataaagt gatgatgaag ttgagagaag aggtttcatt atctgaaatt attgatga
//
LOCUS       DAZGBC010000622          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZGBC010000622 DAZGBC010000000
VERSION     DAZGBC010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.626"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..503
                     /locus_tag="ACHK1J_005646"
     CDS             <1..503
                     /locus_tag="ACHK1J_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO7914735.1"
                     /translation="TLISANTYADQKETAINNRTDGLLTTERQARIAGDAATLATAAA
                     YSDAGDSLTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVT
                     AISSIPYVAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGA
                     GVAGGW"
BASE COUNT          185 a          161 c          134 g          117 t
ORIGIN      
        1 gaacactaat cagtgcgaat acctatgctg accagaaaga aaccgccatc aataaccgta
       61 ctgacggatt actgacgacc gaacgccagg cacgcatcgc aggtgatgcc gcaactctgg
      121 caacagcagc ggcatacagt gacgccgggg attcactgac actgaaacag gcaacagcct
      181 acagcgatat cagtacccgc cgggcactgt cagaggccaa tcgttatacc gacaacaaat
      241 tcagaaacct gagcaataaa attgaacggg cagaaaaaag acttaatgca ggcatcgccg
      301 gagtaacagc aatatcctcc atcccctatg tggctgaaaa ttctttttca tacgggatcg
      361 gcgtgggtaa ctatcagaac gctaatgctg tggccgcagg cattcagtat aaaacatcac
      421 gtaataccaa tatacgcctc aacatctcct gggactcatc tcataacacc gcgattggtg
      481 caggggttgc cggcggctgg taaataatct tgcgtacata cggcattatg gtattccata
      541 atcacattaa ttcagtacgc aacacagccc catccggaac agaccactgg actgagc
//
LOCUS       DAZGBC010000623          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZGBC010000623 DAZGBC010000000
VERSION     DAZGBC010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.627"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>597)
                     /locus_tag="ACHK1J_005647"
     CDS             complement(<1..>597)
                     /locus_tag="ACHK1J_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7914736.1"
                     /translation="GDDDLLTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTEPDP
                     ERVPGAVMRLSCATWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKR
                     QRTRDFLKAWDIENEEGERFSLEDIYWAGLSNPRNRRNEMMTCARGMEQVAEARGDVA
                     FFVTVTAPSRFHSVNDDGSLNPKYCGATVRDASDYLVYR"
BASE COUNT          113 a          177 c          175 g          132 t
ORIGIN      
        1 gcggtaaacc agataatcac tggcatcgcg taccgttgct ccgcagtatt tcgggttcag
       61 gctgccgtca tcgttcacgc tgtgaaaacg ggaaggggct gtgacggtga cgaaaaacgc
      121 cacgtcgccg cgtgcttcgg cgacctgctc cattccccgg gcgcaggtca tcatctcgtt
      181 acggcggtta cgcggattag agagacctgc ccagtagata tcctcaaggc tgaaccgttc
      241 tccctcttcg ttctcgatgt cccaggcctt cagaaaatcg cgggtgcgct ggcgtttttc
      301 gcgccagtcc agtaacgctt catggctgat atataccgat gtcttacgtg acaccagacc
      361 tgcggcgcgt aacagttctt cacgccagat gtcccggagg tgtcttaact gactgttcca
      421 ccatgtcgca caggaaaggc gcatgaccgc acccggcacc cgttccgggt cgggctcggt
      481 gcggcggtcg ggcctgattt ccagcgacgg ccagcagggt ggcgtgatac ccagttttaa
      541 cgccatgtgt gccagtggct ggtaggtcag taaccaggtg agcaggtcat cgtcacc
//
LOCUS       DAZGBC010000624          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZGBC010000624 DAZGBC010000000
VERSION     DAZGBC010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.628"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..526
                     /gene="smrB"
                     /locus_tag="ACHK1J_005648"
     CDS             <1..526
                     /gene="smrB"
                     /locus_tag="ACHK1J_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO7914737.1"
                     /translation="EDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPVKRLIQEQAD
                     ASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHGLTQLQAKQE
                     LGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPKEYGGDAALL
                     VLIEVEEWLPPELP"
BASE COUNT          143 a          135 c          183 g          136 t
ORIGIN      
        1 ggaggaccag gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac
       61 cattgtccat cggccacagc gtaaaaaagt cagcgaagtg ccggtgaaac gcttgatcca
      121 ggagcaggct gatgccagcc attatttctc agatgagttt cagccgttac tgaataccga
      181 aggcccagtg aaatatgtcc gcccggatgt tagtcatttt gaagcgaaga agttgcgacg
      241 cggtgattat tcgccggaat tgtttttgga tttacacggt ctgacgcagc tacaggccaa
      301 gcaggaactg ggggcgttga ttgccgcctg ccgccgtgaa catgtgtttt gcgcttgcgt
      361 aatgcacggg cacgggaagc atattttaaa acagcaaacg ccgctgtggc tggcacaaca
      421 tccgcatgta atggcatttc accaggcgcc gaaagagtac ggcggagatg cagcgttgtt
      481 agtgttgatt gaagtggaag agtggctacc gccggagttg ccatgaatat tagggggcgg
      541 atgcgttttg ccatttcgcc ctgaatgctg ggaccaaatg ccggatgcgg ggtaaac
//
LOCUS       DAZGBC010000625          596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZGBC010000625 DAZGBC010000000
VERSION     DAZGBC010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.629"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..417
                     /locus_tag="ACHK1J_005649"
     CDS             <1..417
                     /locus_tag="ACHK1J_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HFO7914738.1"
                     /translation="DPGAQGPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPM
                     GPPGASDGKSRVVGIRLGNKQTYAPQTDSTTWAVDLDIGAMITGIGGYNDGNKTLIDR
                     VSYRPLQVTFDGSQWRTVSVGEYVSSGASGFEYFPL"
     gene            459..>596
                     /locus_tag="ACHK1J_005650"
     CDS             459..>596
                     /locus_tag="ACHK1J_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914739.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VY"
BASE COUNT          153 a          123 c          199 g          121 t
ORIGIN      
        1 gaccccggag cacagggtcc gaaaggtgaa acgggagcaa caggtcccgc tggtccgcgg
       61 ggagcgcagg gaccgaaagg ggagaaaggc gaccccggtc ccagaggtga gcgtggagaa
      121 accgggccga tggggccgcc cggggcatca gacgggaaga gccgggttgt gggtatcagg
      181 ctgggtaata aacagactta tgcgccgcaa acagacagca ctacatgggc agtggatttg
      241 gatattggtg cgatgattac ggggattggc ggttacaacg acggaaacaa aacccttatt
      301 gacagggtat cttacaggcc gcttcaggtg acatttgatg gttctcagtg gcgtacagtc
      361 agcgtgggtg agtatgtgtc gtcaggagct tcggggtttg agtattttcc attataaagc
      421 gcatcatctt tcagacatgt aaccgggagg actgacagat gcacataaag aatttcagac
      481 aatacacgcc ggaaaatccg gatgtgccgg gtgcgatgta cctgaaatca gaggatggtc
      541 aggactggta tgagtgtcag tcgttatttt cagcagagac gctgaaggtg gtttat
//
LOCUS       DAZGBC010000626          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZGBC010000626 DAZGBC010000000
VERSION     DAZGBC010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.630"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>593
                     /gene="tssI"
                     /locus_tag="ACHK1J_005651"
     CDS             <1..>593
                     /gene="tssI"
                     /locus_tag="ACHK1J_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO7914740.1"
                     /translation="PGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQME
                     GWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRRG
                     AGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKFNW
                     DRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEV"
BASE COUNT          125 a          153 c          226 g           89 t
ORIGIN      
        1 cccggctggg caggacgttt tgatcaggaa ggccagcacc aggattacca gcgcacgcag
       61 tatgaggtgt atgactaccc cggacgtttc aagggcgccc acgggcagaa ctttgcccgc
      121 tggcagatgg agggctggcg aaacaatgca gaaaccgcgc ggggaatgag tcgctcgccg
      181 gagatatggc cgggacggcg aattgtgctg acggggcatc cgcaggcgaa cctgaaccgg
      241 gaatggcagg tggtggcaag tgaactgcac ggcgagcagc cgcaggcggt gccgggaagg
      301 cggggagcag ggacggcgct ggagaaccat tttgcggtga tcccggcaga ccggacatgg
      361 cgaccccagc cgcggctaaa accgctggtg gacggtccgc agagcgccgt cgtgacgggg
      421 ccggagggtg aggaaatctt ctgtgatgaa catggccgag tgcgggtgaa attcaactgg
      481 gaccgttata acccggcaga ccaggacagc tcgtgctgga tccgtgtggc acaggcgtgg
      541 gcaggcaccg gttttggcaa cctggcgata ccgcgtgtgg gtcaggaggt gat
//
LOCUS       DAZGBC010000627          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZGBC010000627 DAZGBC010000000
VERSION     DAZGBC010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.631"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>593)
                     /locus_tag="ACHK1J_005652"
     CDS             complement(<1..>593)
                     /locus_tag="ACHK1J_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7914741.1"
                     /translation="DASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRL
                     MTDTGMYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVW
                     RKLSWDYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNV
                     PDFRSSIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKG"
BASE COUNT          137 a          177 c          129 g          150 t
ORIGIN      
        1 cctttctggt agccacgcac cgtaaaatac tccccttcta acagcttacc gacattgtct
       61 ccgttcccga gaaattcacc atactgcgca ccgatgctgc tccggaagtc aggaacgttg
      121 cgcccctcca gcagataaaa cggacgtacc aggtcatcca gtgcctcccg tccgctgcag
      181 tccagcgtca cacgcccgtt actccagcgg tacagcaggt tttcaatgat gattttcttg
      241 cccagacggc agggattatt ggttctgtaa tcccatgaca atttccgcca gacatcaatc
      301 agtccctgtt caaatgtctg catcttactg gcattcaaat ggcgaaaagt tgccagtaca
      361 ttgtccaggg ttatttcagg acaggtgtcg ctcatcagct ggctgttcca ctcatcacgc
      421 tgacatgaac tcatgaaggt gtacatcccc gtatccgtca tcaggcggtc ccagattttc
      481 cggtcggcaa gacgggtgat attccttttc gcttcttccg gtttatccat gcgacgaacg
      541 gagtcagtca cgcagtcttc aaacccgtag agaaaaatgt cacctgacgc atc
//
LOCUS       DAZGBC010000628          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZGBC010000628 DAZGBC010000000
VERSION     DAZGBC010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.632"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>593
                     /locus_tag="ACHK1J_005653"
     CDS             <1..>593
                     /locus_tag="ACHK1J_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7914742.1"
                     /translation="DASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRL
                     MTDTGMYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVW
                     RKLSWDYRTNNPCRLGKRIIIENLLYRWSNGCVTLDCSGREALDDLVRPFYLLDGRNV
                     PDFRSSIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKG"
BASE COUNT          150 a          131 c          172 g          140 t
ORIGIN      
        1 gatgcttccg gtgacatttt tctctacggg tttgaagact gcgtgactga ctccgttcga
       61 tgcatggata aaccggaaga agcaaaaaag aatatcaccc gtcttgccga ccggaaaatc
      121 tgggaccgcc tgatgacaga tacgggcatg tacaccttca tgagttcatg ccagcgtgat
      181 gagtggaaca gccagctgat gagcgacacc tgtcctgaaa tcaccctgga caatgtactg
      241 gcaacttttc gccatctgaa tgccagtaag atgcagacat ttgaacatgg actgattgat
      301 gtctggcgga aattgtcatg ggattacaga accaataatc cctgccgtct gggcaagaga
      361 atcattattg aaaacctgct gtaccgctgg agtaacgggt gtgtgacgct ggactgcagc
      421 gggcgggagg cactggatga cctggtacgt ccgttttatc tgctggatgg gcgcaacgtt
      481 cctgacttcc ggagcagcat cggggcgcag tatggtgaat ttctcgggaa cggcgacaat
      541 gtcggtaagt tgttagaagg ggaatatttt acggtgcgtg gctaccagaa agg
//
LOCUS       DAZGBC010000629          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZGBC010000629 DAZGBC010000000
VERSION     DAZGBC010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.633"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..248)
                     /locus_tag="ACHK1J_005654"
     CDS             complement(<1..248)
                     /locus_tag="ACHK1J_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914743.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPTTPLTEFSLRKRG"
     gene            complement(248..>593)
                     /locus_tag="ACHK1J_005655"
     CDS             complement(248..>593)
                     /locus_tag="ACHK1J_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914744.1"
                     /translation="AGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHPAFNGG
                     WLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIRRQQFI
                     YPGVPPTKSGEE"
BASE COUNT          142 a          139 c          163 g          149 t
ORIGIN      
        1 tttccccgtt tccttaatga aaattcagtc aggggtgtgg tcggataatt atgccgggcg
       61 cttaattccc tgttgaactg aagtattccg gcaatattca gcagtatgcc ggccagcctg
      121 ccgtcatcat cccgaaggaa ccgacggagt agccggtgct cttctttggc agtactgtca
      181 gcttttccgg ccaggtgtgc gaggatgagt gttataagcg tctccgggga tatgcggtga
      241 attttcatca ttcttctcct gattttgtcg ggggtacacc cggataaata aactgctggc
      301 ggcgaatgaa aaaatcacag aagggcatat ccggtaattc cgggagaccg gacacggcaa
      361 tatccatcca tgagagatag ttatcgacgc tcactgtgac ggtgatgctg ttatgggtac
      421 gggatctttc atgaatttct gcagccagaa agagcagcca gccgccgtta aatgccgggt
      481 ggcggagaat gaaccgggca atctcttttt catctccgtc aggcggaata tcgtcagtca
      541 gcgtccggat aataaagcgc cggaatgcat ctgcggaaaa ctgatatccg gca
//
LOCUS       DAZGBC010000630          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZGBC010000630 DAZGBC010000000
VERSION     DAZGBC010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.634"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..386)
                     /locus_tag="ACHK1J_005656"
     CDS             complement(<1..386)
                     /locus_tag="ACHK1J_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HFO7914745.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSP"
     gene            complement(383..>592)
                     /gene="tssK"
                     /locus_tag="ACHK1J_005657"
     CDS             complement(383..>592)
                     /gene="tssK"
                     /locus_tag="ACHK1J_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7914746.1"
                     /translation="IYGAALRGIPLIPVSRVPAALPVRMENQYFALDMESPAAHEMLE
                     QGVCMFYVPELLGALELELFAVLRS"
BASE COUNT          137 a          186 c          138 g          131 t
ORIGIN      
        1 tccggtgaag gctgacgcag aacctctgca atgcgatccc acagtgcttc accagcccga
       61 agagaaccaa aaaagcgggc ctgaagcggc actttacgcc aggccgcctg gccttcgtca
      121 acgtccggct ccccgccttc cccgcttttt tcaggcttac ggctcatcac cgtttcgtcc
      181 aggagggcac attgtgcata ggtaatatgc tccacactgg catcgtcata accggcacgc
      241 tccagcgctt cccgcacact ttcgacctgt ttaacgcatt tgttgtaaag cgcagtgcca
      301 tcgggcgtga gtgcaccttt tttcagcatg gtgacggtaa gccatgtctc catcatcagc
      361 tggtcgatat caatatcctg tctcatgagc gcaataccgc aaacagttca agttccaggg
      421 caccaagtaa ctcaggcaca tagaacatac acactccctg ctccagcatt tcatgtgcag
      481 cgggactttc catatcaagt gcgaagtact ggttttccat acgtacaggc aacgcagccg
      541 gaaccctgct caccgggatc aaagggatcc ccctgagtgc cgcgccatag at
//
LOCUS       DAZGBC010000631          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZGBC010000631 DAZGBC010000000
VERSION     DAZGBC010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.635"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>592)
                     /locus_tag="ACHK1J_005658"
     CDS             complement(<1..>592)
                     /locus_tag="ACHK1J_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO7914747.1"
                     /translation="SGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINP
                     QNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYENKKLK
                     KLFTLLDKRDMILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMR
                     NMEGNKDSRINKNVVVLAGNHEINFNGNYSARLANHK"
BASE COUNT          188 a          125 c           85 g          194 t
ORIGIN      
        1 tttatgattt gctagtcgtg cactatagtt accattaaaa ttgatttcat gatttccagc
       61 cagaacgacg acatttttat ttatacgact atctttatta ccctccatat ttcgcataga
      121 atttagcaaa gtaaggatat atttatcacc aaaaatagta ctgaatctat cgcctgtatg
      181 atccccaagg aagatacaag gggtaatcgc atcgcctttt aaaaaaacat gttgatgcaa
      241 ctcattcaag atcatgtctc tcttatctaa taaagtgaag agttttttca attttttatt
      301 ttcatatgcg aatgaagcca tgtcttcgtg tttcaataaa tcacataata aactccaccc
      361 cttttccctg atgcctatat gccctgaaac gagcaatgca taaagtacag cgcctactct
      421 tccatctgta tcgccaaaat aagccgtgtt ggcattttgt gggttaatca caccacactg
      481 tttataaaag tctatttttg aaaaaacaga ttcaacatag ctatcaaact tttccttagt
      541 aaacgtctct ttagagacag cccaacgcca gtcaggtttg aaattaccac ta
//
LOCUS       DAZGBC010000632          590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZGBC010000632 DAZGBC010000000
VERSION     DAZGBC010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.636"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /locus_tag="ACHK1J_005659"
     CDS             <1..354
                     /locus_tag="ACHK1J_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914748.1"
                     /translation="DNVERALDKINILIDKERVYDGETLAELITENKILSSRESKENL
                     IGLFTGRHKYTLLQCIEKLGVLVDYVISPADDIKNVMMLYGNNAENKNRRRLIYNALT
                     IICGDDTKNNQSDPR"
BASE COUNT          196 a           89 c          126 g          179 t
ORIGIN      
        1 gataatgtag agagggcgtt agataagata aatattctga tagataaaga acgtgtttac
       61 gatggtgaaa cgttggctga attaattaca gagaacaaaa tattatccag tcgtgaaagt
      121 aaagaaaacc tgataggatt atttacgggc agacacaaat atactttact gcaatgtatt
      181 gagaagcttg gcgtattggt tgattatgta atatcaccgg ctgatgatat taaaaacgtc
      241 atgatgttgt atggaaataa tgctgaaaac aagaacagac gcagactgat ctacaacgca
      301 ttaaccatca tttgtggtga tgatactaaa aacaaccagt cagaccctag atgatattat
      361 cggaacgtgt ttttggcatc cgatgtttgt atatccctgc tattgttgct agtcggctag
      421 caacaatagc agagggggca tgcatgtctg aaaaaataaa aaatcgcaac acattgattt
      481 ttatcttgtt tgcattttgc aaatactatc tctcgttttt gtgattagtg tcatgtttta
      541 tcgtctcgcg gataaccgtg aggagtgaac agtaaccctg attatatgat
//
LOCUS       DAZGBC010000633          590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZGBC010000633 DAZGBC010000000
VERSION     DAZGBC010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.637"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..509
                     /locus_tag="ACHK1J_005660"
     CDS             <1..509
                     /locus_tag="ACHK1J_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7914749.1"
                     /translation="SAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICR
                     GATRVDGNPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQKAGIASFCPY
                     NIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRTRSNNCYGQVIRRDQESA
                     LACWGIDQ"
     gene            506..>590
                     /locus_tag="ACHK1J_005661"
     CDS             506..>590
                     /locus_tag="ACHK1J_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7914750.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRD"
BASE COUNT          139 a          132 c          178 g          141 t
ORIGIN      
        1 tgtcggctgc cgttctggcg ttgattgccg caggggcttc tgcgcctgaa atcctcgacc
       61 agtttctgga tgaaaaggaa ggcaaccaca ccacggcata ccgtgatggt gcgggtatct
      121 ggaccatctg ccgtggagcc acccgggtgg atggtaatcc tgtgattcct ggcatgaagc
      181 tgtcgaagga aaaatgcgac caggttaacg ccattgaacg ggataaggcg ctggcatggg
      241 tggagaaaaa catcagagtg ccactgactg aaccccagaa agcggggatc gcgtcattct
      301 gtccttacaa cattggcccg ggtaagtgtt tcccgtcgac gttttataaa cgaattaatg
      361 caggcgatcg caggggagcg tgtgaagcga ttcgctggtg gattaaggac ggtgggcgtg
      421 attgccgcac acgttcaaat aactgctacg gacaggttat tcgtcgtgac caggaaagcg
      481 cattagcttg ttgggggata gatcagtgag aagagtaacc gcgattattt acgctctggt
      541 tatctgcatc atcgtctgtc tgttatgggc tgttaatcat taccgcgata
//
LOCUS       DAZGBC010000634          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZGBC010000634 DAZGBC010000000
VERSION     DAZGBC010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.638"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..449
                     /locus_tag="ACHK1J_005662"
     CDS             <1..449
                     /locus_tag="ACHK1J_005662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914751.1"
                     /translation="RLQQIASSTNDNNLKDTSMELTVDLLKSLNNIRQNATIYVHAYH
                     YTDDEKCVMVPRISLPSLKLTPLQSEIIIRQLLNGVNYIRHDGDVQGYEEKNVATFTI
                     QEEGHHRFKADLSQNGESDIASNVLNHWLHRIHKTDIIVLSVVLTE"
BASE COUNT          208 a          116 c          109 g          153 t
ORIGIN      
        1 aacgccttca acagatcgca tcatcaacga atgataacaa cctgaaggat acctcgatgg
       61 agttgactgt agacctgctg aaaagcctta acaacatcag gcaaaatgcc accatatatg
      121 ttcacgcata ccactatact gatgatgaaa aatgcgttat ggtgccacgg atatctttac
      181 catcactgaa attaactccg ttgcaatcag aaataattat tcgacaactt cttaatggag
      241 tcaattatat aaggcacgac ggggatgttc agggatatga agaaaaaaat gtggcaactt
      301 tcacaataca ggaggaggga caccatcgtt tcaaagcaga cttatcacaa aatggggaaa
      361 gtgatatagc cagcaatgta ctaaatcact ggctacatcg tatacataaa acagatatca
      421 ttgttctgtc tgttgttctt acagaataaa tccagttacc tcctgaatgt tacaaggttt
      481 tatatttatc ctatgagaat acgcacgtga aaactaataa aaaaggcaca acatggcaca
      541 ttttttaccg tgaaaattca ggcgcggaag ttttgcttga aatacc
//
LOCUS       DAZGBC010000635          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZGBC010000635 DAZGBC010000000
VERSION     DAZGBC010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.639"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHK1J_005663"
     CDS             complement(<1..242)
                     /locus_tag="ACHK1J_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7914752.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFEL"
BASE COUNT          205 a          122 c          124 g          135 t
ORIGIN      
        1 agttcaaacc aggtcaccac acctttcacc cggcgctgta catcatcacc ctgccatatt
       61 gtcaggtagg ccattttgtc cagcacctgc gcaaattcaa gggagagaaa ctgctggctg
      121 accagggaga gatcgaggga aaagagcgaa gagagtgact gggtgagatg aaaggaaacc
      181 accgcaaaag cgtccggtgg caggccgtcc acttccagtg tgaaacgtaa tccggttgac
      241 atagcaacct ccggtaaaat gaataataat ccgcaccgta caactgcgga taatgagata
      301 agcgggacga cttaagacgt tattagggaa ggaacaaaaa atgctatgaa tatgtgagaa
      361 ttttgtttaa atattacttt acagaaacgg gtgtctgttc agtcaaaaaa ataatcacaa
      421 gctcaactta ttgtttcctg acgttattca ggacaggtgc gcacatcaca gcgagtaaca
      481 aaaacgcaaa gtaaaaccaa tattcataat atgtttttat tcacaactaa ataacccaat
      541 taacatcatt aaaatgaatg attgcttaaa aaatatatgc taccgc
//
LOCUS       DAZGBC010000636          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZGBC010000636 DAZGBC010000000
VERSION     DAZGBC010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.640"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..264)
                     /locus_tag="ACHK1J_005664"
     CDS             complement(<1..264)
                     /locus_tag="ACHK1J_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO7914753.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMS"
BASE COUNT          166 a          118 c          166 g          134 t
ORIGIN      
        1 ggacataaca tccagccact ggctgaaagt gatggttgcc gcgttgttag cagtgatggc
       61 cagcaggttg taacgggggg cggcaaagtt gtgaaagggc ccccagtcaa tcatgcccca
      121 cagtgtgtgc aggatcaggc aggaggcgaa gacgatttca ggtaaaaagc gccagatgat
      181 aatcgcaaag agcaggcata tggcaccgac gatataccat atagccattg gatcttcggc
      241 ggcagacgtc tgttggtaag acacggggat tcctccatag atgaatggat aaatttcggg
      301 attaagtcgg gtggcatgaa tgcgaatcag cgccggtaat aaccggacag gaatgagttc
      361 aacaggacat tttcacagag aagatcgaac ggcagaaggt ggtgggagac aggcacaaaa
      421 aaaccgactg gtgaggccgg tttttttgct attccggagc ggagttggtg ctctgaccgg
      481 aggaagatac tctacatcca acatccataa tattatggca ccagacattt gttgtctagt
      541 ttgacttcag attgcctgtc aatattcatt tctttaaatg aatt
//
LOCUS       DAZGBC010000637          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZGBC010000637 DAZGBC010000000
VERSION     DAZGBC010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.641"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>584)
                     /locus_tag="ACHK1J_005665"
     CDS             complement(<1..>584)
                     /locus_tag="ACHK1J_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO7914754.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHYSTISRMEDALKYL"
BASE COUNT          128 a          163 c          144 g          149 t
ORIGIN      
        1 tacagatact tcagggcatc ttccattctg ctgattgtgg agtaatgtat cggcagccct
       61 tcatgggtta acaaatcagc cagttcacga agagacactg ttctgcccag ttgttcctca
      121 tggatttgtc ttgccttgtg gaccccctgc gccttctcaa taaaagtcag ttcaccgcga
      181 acttcatttt cagccagatg accaatcaca cactggagtc gacccggcca gggtttaaac
      241 agcacatgaa tacggtaaaa acgcggatcc cctgtttctt tccagagttc cgtcagtatc
      301 tggtaacggg tgtttccccc gtcactgaag atatacacat ccggctcact gtccggcata
      361 cgggtgactt tgggcaccgt atccagcccg cgggcatgaa tggaattttt gatatcgtca
      421 taacgcggat tgcgcgtggt gcgcgggtta tccggattcg gacgtaactg gtccagcgtc
      481 agaaccatgg gcgtttcccc cattgccacc acattgccgg caccagtggt ctgacgaccg
      541 ggctgcataa tagccgcccc gagatccagt ggtttatgtg ccat
//
LOCUS       DAZGBC010000638          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZGBC010000638 DAZGBC010000000
VERSION     DAZGBC010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.642"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..113)
                     /locus_tag="ACHK1J_005666"
     CDS             complement(<1..113)
                     /locus_tag="ACHK1J_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO7914755.1"
                     /translation="MNIEELKTKTEADISEYITKKIIELKKKTGKEVTSIQ"
     gene            complement(286..516)
                     /gene="ynfN"
                     /locus_tag="ACHK1J_005667"
     CDS             complement(286..516)
                     /gene="ynfN"
                     /locus_tag="ACHK1J_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO7914756.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAMRLLN"
BASE COUNT          173 a          112 c           96 g          200 t
ORIGIN      
        1 aactggatac tggtaacttc tttcccggtc tttttcttaa gctcaatgat tttttttgtt
       61 atatattcag aaatatctgc ttctgttttt gtttttaact cttcaatgtt catcatttcc
      121 tcttttagtc tgttatgact ttcctggtac acagtagtgt caattatatg gagcaaacgt
      181 ataaaagata agatgaaaca tcgcaataat caacatacga cggtctaaat ttcacacaaa
      241 cagataaaga tgattatcat ttattatcaa tgcattagaa tcaaatcaat tcaagagtct
      301 cattgctgct tccagaattt cttctgaagt tacatgtcga tccgctgcta cataaatgac
      361 tttatgatct ccggtcagag atggaaaccc tgcagccatt acagtgaggt gtgttgtttc
      421 gccatttgga tattcacgca tgatggtgtt aactccggtc atcactggca ctaccattgc
      481 tggttcagag ttaaaaaaaa ctattatttt tttcataatg ttaccgtaat atgtgagtat
      541 ccatcgacta gacactaagc aaaaaagctc ccgaaggagc c
//
LOCUS       DAZGBC010000639          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZGBC010000639 DAZGBC010000000
VERSION     DAZGBC010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.643"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..534
                     /locus_tag="ACHK1J_005668"
     CDS             <1..534
                     /locus_tag="ACHK1J_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFO7914757.1"
                     /translation="HWVAEPSASQGMVVSPDFLRAINQLAELSDIVFSQGDAGVHFEL
                     MARPSRDVARVQLTLDEQNLDYFNQMESWQSFTWPGNTYYPGVSLSWRSVSSGMQLYA
                     SHQGNWGFIRLLDEALITPLDASRTQLVWITADGNPLKFVMRSELGDGPLALLKLQGF
                     TLPTAIFNVAAGTEGVE"
BASE COUNT          121 a          151 c          171 g          137 t
ORIGIN      
        1 cactgggtcg ctgagccttc cgcaagccag gggatggttg tcagtccgga tttcctccgg
       61 gcgattaatc agctggcaga gctgtcagat atcgtgttct ctcagggcga tgcgggggtt
      121 cacttcgaac tgatggcgcg tccttcacgg gatgttgcca gagtgcagct tacccttgat
      181 gaacagaatc tggattactt caatcagatg gaaagctggc agagcttcac ctggccgggg
      241 aatacttact atccaggtgt gtcgctcagc tggcggagtg taagcagtgg tatgcaactc
      301 tacgccagtc accaggggaa ctgggggttt attcgcctgc tggacgaagc cctgatcacg
      361 ccacttgatg ccagccgcac acaactggtg tggatcactg ccgatggtaa tcctctgaaa
      421 ttcgtcatgc gcagcgagct gggcgacggt ccgcttgcct tactgaagct acaggggttt
      481 acgctgccca ccgccatttt taacgttgcc gccggcacgg aaggtgttga gtaaaaatga
      541 acaggggcat ttatccgacg ccggccttta ctgaagatct
//
LOCUS       DAZGBC010000640          579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZGBC010000640 DAZGBC010000000
VERSION     DAZGBC010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.644"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(241..447)
                     /locus_tag="ACHK1J_005669"
     CDS             complement(241..447)
                     /locus_tag="ACHK1J_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO7914758.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          163 a          128 c          111 g          177 t
ORIGIN      
        1 gctcatcctt gttttatcga gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccgtcagat agaaactatt aggaaaatat ttatatatat
      121 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      181 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      241 tcaataaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      301 tttcgaccag acgcgatgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      361 gaaaggcagc gccagaacgc ccgctgcaac cgcataagta cggcgtaaaa agataagcca
      421 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      481 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      541 tactactcat ccgaccactt atttttgctt attgatggt
//
LOCUS       DAZGBC010000641          573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZGBC010000641 DAZGBC010000000
VERSION     DAZGBC010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.645"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..>573
                     /locus_tag="ACHK1J_005670"
     CDS             105..>573
                     /locus_tag="ACHK1J_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO7914759.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIIT"
BASE COUNT          133 a          116 c          165 g          159 t
ORIGIN      
        1 tgtcagtaat attcattttt tgtaaaaggt aaatataaat tattctcatt attgtttgta
       61 tttgtgtatt gtcttgccga ttaacatgat cggagattag taatatgcga ataaccactc
      121 tggcttccgt tgtcattccc tgtctcgggt tttcagccag cagcatggca gcagaggatg
      181 tgatgattgt ctcggcatcc ggctatgaga aaaaactgac caacgcagcc gccagtgttt
      241 ccgtaatcag tcaggaggaa ttgcagtcca gccagtacca cgatctggcg gaggcactga
      301 gagctgtgga gggtgtggat gttgaaagtg gtacgggtaa aaccggtggg ctggaaatca
      361 gcatccgtgg aatgcccgcc agttacacgc tgatactgat tgatggtgtc cgtcagggcg
      421 ggagcgggga cgtgacgcct aacggttttt ctgccatgaa tacagggttc atgcctcctc
      481 tggccgcgat tgagcgtatt gaggttatca gggggccgat gtccacactg tatggctctg
      541 atgcgatggg tggtgtggtg aatatcatta cca
//
LOCUS       DAZGBC010000642          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZGBC010000642 DAZGBC010000000
VERSION     DAZGBC010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.646"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..374
                     /locus_tag="ACHK1J_005671"
     CDS             117..374
                     /locus_tag="ACHK1J_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7914760.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            377..>572
                     /locus_tag="ACHK1J_005672"
     CDS             377..>572
                     /locus_tag="ACHK1J_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914761.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGL"
BASE COUNT          150 a          128 c          145 g          149 t
ORIGIN      
        1 cccggaaaca ttcataataa aaatccagac aatcactatc gatggcagag cccagcataa
       61 acatgggaat taaagagtga tgaattagtt aagagataag aaagaggaat ttaataatgc
      121 ttcatattat tcgtaaaggc gataaaacta ctcacggagg ttccgttctg accgcttctg
      181 aaacgatgaa attcggtggg ataggcgtcg cccgtaaagg cgataaagtg tcatgtccgg
      241 gtcatggtcc taccatcatc atagaaggca atcctgatta tcttgaccat ggtatcccgg
      301 tggcatttca cggtcataaa tgcgcctgtg gttgcacatt gatcagttca tttgccgcag
      361 caaaggttgc ctgattatgc ctgtctggct ggatgccatc cctgaaaagg ccccaaagat
      421 agcgcgcccc aatactcgcc gctggctgtt gttcctggca tttatcatgc tgggggggat
      481 ggcgctcaca ttatggtgct ggacgtcaga acgaacagga tttgtcttct ggttcaccgc
      541 tctggggctt ccattttgca cgtgggggct ta
//
LOCUS       DAZGBC010000643          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZGBC010000643 DAZGBC010000000
VERSION     DAZGBC010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.647"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>571
                     /locus_tag="ACHK1J_005673"
     CDS             <1..>571
                     /locus_tag="ACHK1J_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7914762.1"
                     /translation="RGLLQQFGETIRVDFQHKNDIEEAFKYASINNMVPISISDSVGS
                     MGGVAPVEFIVRMANRFDGYAYIDDAHGMSIYGKNGSGYVMSCLDNKLDDRIIIAGSL
                     SKAFGSHGGFIACNYPDTINFIKTYGTTYAFGGPPSLPGIAACIAAADLHLDGTVSLR
                     QNDLQQVIKYFDDKFNETEVINKGTTMPIR"
BASE COUNT          185 a           96 c          117 g          173 t
ORIGIN      
        1 agaggactat tacaacagtt cggtgaaaca atcagggttg actttcaaca taaaaatgat
       61 atagaagaag cgtttaaata tgcttcaata aataatatgg taccaatatc tatatctgat
      121 agtgtcggtt cgatgggtgg ggtggctcct gtcgaattta ttgttagaat ggctaacaga
      181 tttgatgggt atgcttacat tgatgatgct catggcatgt caatatatgg gaaaaatggc
      241 tccggatatg taatgtcttg tctggataac aaacttgatg acagaattat tatcgctggt
      301 tcattatcta aagcgtttgg ctctcatggt ggatttattg catgcaatta tcctgacacc
      361 ataaacttta taaaaacgta tggaactacc tatgcttttg gtggtccacc atcattacca
      421 ggaattgctg catgtatcgc agcagcagat ttacacctcg atggcacagt atccttgcgt
      481 caaaatgact tacaacaagt tataaaatac tttgatgaca aatttaatga aaccgaagtc
      541 ataaacaaag ggactacaat gccaatacgt g
//
LOCUS       DAZGBC010000644          570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZGBC010000644 DAZGBC010000000
VERSION     DAZGBC010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.648"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..509
                     /locus_tag="ACHK1J_005674"
     CDS             42..509
                     /locus_tag="ACHK1J_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914763.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
BASE COUNT          173 a          112 c          155 g          130 t
ORIGIN      
        1 gaatgagcac tactgtcatt cacatcaaag gggaatgaga tatggctatt gcagagagaa
       61 cgggtatctc ctcgcgcggc gatacttacg aaatctggtt gggagcaaac tgttatcact
      121 accgctctga tggttatgca gtgcttctga aagaaggcag gaatcgtgct tatccatgtc
      181 gggtgacgag tgaaacgatt aaatctgcgc tggcagcaat tgttgaattg caacagaaat
      241 taactgcggc agagaagaaa aaggagtgtg agaaccacgg ggttcttttg ccagctgaga
      301 tgttaattcg ctgtctggaa gatcaaacag ggggacggtg gatccgtgca catgaaccag
      361 taatgctgat gaagcgatta atcagtaacc tgaaaaaagc cagaccagaa tgtaccgttc
      421 ctgatgaagc gctggcatat ctccggcgta actgtctggt gaatatgggc gatatacccg
      481 ttaccagcag acggatgtct gacgtataac aaaaaggaaa agtgtaatga cattacccgt
      541 agacagcctg attgcgtaca cgctggaaaa
//
LOCUS       DAZGBC010000645          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZGBC010000645 DAZGBC010000000
VERSION     DAZGBC010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.649"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..434
                     /locus_tag="ACHK1J_005675"
     CDS             <1..434
                     /locus_tag="ACHK1J_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914764.1"
                     /translation="SLETTPGWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQE
                     ENCYPDTDDLQYVSWLSPEEAENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATT
                     LYTDNSGHPQYDIVNTLKKHIGKDEYNQYESLRQRIEWVCL"
BASE COUNT          183 a           90 c          114 g          181 t
ORIGIN      
        1 ggtcgttaga gacgacacca ggatggattt atttatcgga tcaacttgat aatgcggtgc
       61 attgggggaa taaaatagca tgcctttcaa aagatgaatt atttttctat attttcagag
      121 ttcaggttca ggaagaaaat tgttatcctg atacagatga tttgcaatat gtgtcgtggt
      181 tatctcctga ggaggcagaa aacacagacc tgtatacatg tctttctctt tgccattcct
      241 gccgaactga caaagaactg tatacaggtt gtgaaatatc agattatgca gtccttcctg
      301 caacaacact gtatactgac aattcaggac atcctcaata tgatattgtt aatacgctta
      361 aaaaacatat aggcaaagat gagtataatc agtatgaatc acttcgtcag cgtattgaat
      421 gggtttgttt ataagtgatc ttattaagat gtacaacaat agattttctt cttttgtttt
      481 gtataaatac cggaggaata taaaagaaac ggagactgca gagacagaga tgaagttgtt
      541 ttccccgttt ccttaatgaa aattcagt
//
LOCUS       DAZGBC010000646          564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZGBC010000646 DAZGBC010000000
VERSION     DAZGBC010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.650"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..183
                     /locus_tag="ACHK1J_005676"
     CDS             <1..183
                     /locus_tag="ACHK1J_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO7914765.1"
                     /translation="TVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRAL
                     QRSVLPDDDEGDENDE"
     gene            176..>564
                     /locus_tag="ACHK1J_005677"
     CDS             176..>564
                     /locus_tag="ACHK1J_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO7914766.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQL"
BASE COUNT          110 a          140 c          190 g          124 t
ORIGIN      
        1 acagtcctcc ccgtgagtct gaaagacctg cttacagggg gcagtgcacc ggaagcgtgg
       61 ccactgctgg atgacgacca tgccgtgaag ctgctgcgtc ttctgagtgt gctgcgccgc
      121 ctgcgagccc tgcagcgcag tgtattaccc gacgacgatg agggggatga gaatgatgag
      181 tgatatgtcg catcaggaac gtctgatcag gcgggtcagg aaactgctgg agctgagccg
      241 taataacagc aatgcccatg aggccgggct ggcgcttgca agggcgcaac agctgatggc
      301 gaaacacggt atcacggagc aggatgccgg actttcttcg gtgcagacct cttcatcaca
      361 gggggcacct tctgatgctg aaaaagttcc ggcgtggatg tttcgtctgg tcatggtggt
      421 gacacagaca tttggctgcc gggcgtatta cgactggcgg gcggtgcccg gaggccggta
      481 tcgccggtgt gtgacgtttt acggtttcag tgaacgtcct caggtggcgg cttatgcgtt
      541 tgatgttctg tgccgtcagc taag
//
LOCUS       DAZGBC010000647          564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZGBC010000647 DAZGBC010000000
VERSION     DAZGBC010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.651"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>564
                     /gene="espL4"
                     /locus_tag="ACHK1J_005678"
     CDS             <1..>564
                     /gene="espL4"
                     /locus_tag="ACHK1J_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO7914767.1"
                     /translation="FLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKME
                     SNGISVMAAILLVDNHALTARLRIKNTTEGYTHYVVSVYDPNVTNDKIRIMSESKEDI
                     KHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEITPPLSPATM
                     NLLMAIGQNHQLTQLMIQLQKMPELHRT"
BASE COUNT          188 a          108 c          112 g          156 t
ORIGIN      
        1 tttctcacgc acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt
       61 cctgaggggc gttgtgttgc tgcctgcggt gatttcgggc tagttatgaa ggcatatttt
      121 gacaagatgg aatcaaatgg catcagtgtt atggctgcca tattattggt agataaccat
      181 gctttgacgg cccgactaag aataaagaac acaactgaag gatataccca ttacgtggtt
      241 tcggtttatg atccaaatgt aactaacgat aaaataagaa tcatgagcga aagtaaagag
      301 gatattaaac actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg
      361 tcaaatgatc atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag
      421 ctattgatgt taaaaggatc tgtggatgaa ataacccctc cattatcacc tgcaacgatg
      481 aatttgctaa tggcaattgg tcagaatcac caacttacgc aactgatgat tcaactccag
      541 aaaatgccag aactacatag aaca
//
LOCUS       DAZGBC010000648          556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZGBC010000648 DAZGBC010000000
VERSION     DAZGBC010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.652"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>556
                     /locus_tag="ACHK1J_005679"
     CDS             <1..>556
                     /locus_tag="ACHK1J_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7914768.1"
                     /translation="NEENPHHLHQPYRLPGQQYDEESGLYYNRNRYYNPLLGRYITQD
                     PIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKR
                     LNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSH
                     SEMIEDNKKDLAVNEHGLTCPLTQD"
BASE COUNT          171 a           90 c          149 g          146 t
ORIGIN      
        1 aatgaggaga acccgcatca cctgcaccag ccgtaccggc tgccggggca gcagtatgat
       61 gaggagtcgg ggctgtatta taaccggaac cggtactaca atccgttgct gggacgatat
      121 attactcagg acccgattgg gctggcgggg ggatggagtc tgtatgcgta tccgctgaat
      181 ccggtgaatg gtattgatcc attagggtta agtcctgcag atgtagcgtt aatgcgaaaa
      241 aaagaacaac taaaccatca aagagcatgg gatatattat ctgatactta tgatgatatg
      301 aagagattaa atttaggtgg gactgatcaa tttttccatt gtatggcatt ttgtcgagtg
      361 tctaaattaa atgacgctgg tgttagccga tcagcgaaag ggctgggtta tgaaaaagag
      421 attagagatt acgggttaaa tatgttcggt atgtacggca gaaaagtaaa gttatcccat
      481 tctgagatga ttgaagataa taaaaaagac ttggctgtaa atgaacatgg gttgacatgt
      541 ccattaacac aagatt
//
LOCUS       DAZGBC010000649          552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZGBC010000649 DAZGBC010000000
VERSION     DAZGBC010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.653"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          191 a          105 c          100 g          156 t
ORIGIN      
        1 gtatcataaa tacctgtcca cacttaagat tttcctgcgg aaaacaaata ttaacatatt
       61 ttatgtattc cgcatggtga tgatttatac acctgcactg aatttttctg tatctccaga
      121 gctgaatcga taaacagaag tggtaataat gaaagttaat cacataaaaa agaggttacc
      181 gccttataat gataagagtt cattatccat tccatccaga caaaacctgc ctgtgtataa
      241 aaaataagaa ccatgtcaca aaaacattgc attccggcaa gttatgacag aattactgaa
      301 aaccgttatc tgtagttatt gtagtttctg atatatgctc atccacccgg aaatacgaca
      361 tggtcctgac tggcgttcag ttcaaagtgt aatttattat tcacacactg agactgaaaa
      421 acggatggcg tggatgagta cacagcctga agagtataaa aggaacagct ccggattgtt
      481 acccgggatt actatagttc accacacaac cgtaaacctg gttgtgtggt gaaacagtaa
      541 catattaaga at
//
LOCUS       DAZGBC010000650          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZGBC010000650 DAZGBC010000000
VERSION     DAZGBC010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.654"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>536
                     /locus_tag="ACHK1J_005680"
     CDS             <1..>536
                     /locus_tag="ACHK1J_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7914769.1"
                     /translation="FEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLH
                     ELWLQSGTGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPE
                     KTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGD
                     NFIQVDFDTPWCQPESDV"
BASE COUNT          115 a          129 c          165 g          127 t
ORIGIN      
        1 ggtttgaacc gtgccccgga ctgacggctg ccggacgtgg cgttgtatcg ccggagaata
       61 tcgcgttcac ccgctggctg acacacctgc agaacggtgt actgctggat gagcaaaact
      121 gcctgatgct gcatgaactc tggctgcaga gcggtactgg ccagcgtcgc tgggaaggat
      181 taccggacga tgtcagggat accatcaccg cgcttttcac cgcaaaaaga ggtgactggt
      241 gtggcttctg gagtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt gacaacgtac
      301 tgccggaaaa aaccatgccg tttgacctgc tgacggttct gcccacccgc cttgatgttg
      361 aagtgaatgg ctttaacggt ggtgttctga acggtgttcc ttctgcatat cactggtata
      421 ccgaacagta tggcgtgaag tggccttgtg ggtatgacct gaatatcagc agtcagggag
      481 acaacttcat tcaggttgat ttcgacacgc cgtggtgtca gccggaaagc gacgtt
//
LOCUS       DAZGBC010000651          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZGBC010000651 DAZGBC010000000
VERSION     DAZGBC010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.655"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..121
                     /locus_tag="ACHK1J_005681"
     CDS             <1..121
                     /locus_tag="ACHK1J_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7914770.1"
                     /translation="PRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK"
     gene            211..>536
                     /locus_tag="ACHK1J_005682"
     CDS             211..>536
                     /locus_tag="ACHK1J_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7914771.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDN"
BASE COUNT          127 a          154 c          125 g          130 t
ORIGIN      
        1 tccccgccat cagcataccg tcacgctgta tgccagaggg ctgacctgcg aagccgacac
       61 cctcggcagt tgtggctacg tttatatggc tgtttatccg acgcccgaaa cgaaaaagta
      121 actctccaga ataaccctct gctccggcct ggtgttttca ccacaccact ttcccatttt
      181 tcatctttgc atatcaggaa aatcttcagt atgaaaacat tacctgtatt acccgggcag
      241 tcggccagtt cacgcccatc tcctgttgaa atctggcaga cactgctcac ccgactgctg
      301 gaccagcact atggccttac gctgaatgac acaccgttcg ctgatgaacg tgtgattgag
      361 cagcatattg aggcaggcat ttcactgtgt gatgcggtga attttctcgt ggaaaaatac
      421 gcgctggtgc gtaccgacca gccgggattc agcgcaggaa cttcgtcgca gttaatcaac
      481 agcattgata ttctccgggc acgccgggca acaggtctga tgacccgcga caatta
//
LOCUS       DAZGBC010000652          531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZGBC010000652 DAZGBC010000000
VERSION     DAZGBC010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.656"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>531)
                     /locus_tag="ACHK1J_005683"
     CDS             complement(<1..>531)
                     /locus_tag="ACHK1J_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HFO7914772.1"
                     /translation="CILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRTEI
                     STPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAGFV
                     NTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSSYL
                     ALMEFSGNAMTRDASRA"
BASE COUNT          165 a          116 c          115 g          135 t
ORIGIN      
        1 gctctggatg catctctggt catggcattt ccactaaact ccattaacgc cagatatgat
       61 gaaaccagtg agtgacgact gatttgcatt ccggaacgtt ccagcgctgc gacacgttgc
      121 agagtggtat aactgctgtc cgttgtcatg gaaacagttg tcacaccggg cactgatata
      181 tgtgcaaaat cagaaaatct gtagaaagta tttgttgccg tattaacgaa tccggctaca
      241 tataaattat tttgctcaac aatcagacga agatggtcaa aacgcgcctg atagacatca
      301 agccctcgta tatccacagc aaaataactg cccggtgggg tgtggttaat aacagacacc
      361 gatgtggtcc cctgagatat atgctcaaga ggagtcgata tttctgtccg tatactattt
      421 aacgaagaga cataactttg ttgagtcgaa aagtctatcg taaattcccg ggaataggat
      481 accgaagaaa agcccagtaa caggcacagt acccatttta acaatataca c
//
LOCUS       DAZGBC010000653          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZGBC010000653 DAZGBC010000000
VERSION     DAZGBC010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.657"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..470
                     /locus_tag="ACHK1J_005684"
     CDS             <1..470
                     /locus_tag="ACHK1J_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO7914773.1"
                     /translation="WFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLKVI
                     ARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNVCL
                     NIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
BASE COUNT          155 a          127 c          113 g          135 t
ORIGIN      
        1 aatggtttta tctctcatct cttttcttat tctgtctccc ggcgactgcc gccgtaaaca
       61 gtaacacact ggatagctgc tttattcagg caggaaaacg ttactccatc gatcccggac
      121 tactgaaagt aattgcccgt aaagaaagca gcctgaatcc ccgggccatc aatcataaca
      181 gaaatgccag cggaaaaatt atcagcacag attatggcct gatgcagatt aattccacac
      241 atattcccgg actgaaaaga atgggaatta tccgaagtga aaatgaactg ctcagtaatg
      301 tttgcctgaa tatacagaca ggtgcatgga tccttgcccg gcattttcag cagtgtggtg
      361 tgaactggga gtgtctcggt agctacaacg ccggatttaa taaaaataac actcaccgca
      421 gaatgaaata tgcccggctg atttatgccg catatatgca gggacgttaa taacggagaa
      481 tacgagccat gttcctcatc atcttactcg tgatctccgc tacgctgacc
//
LOCUS       DAZGBC010000654          529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZGBC010000654 DAZGBC010000000
VERSION     DAZGBC010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.658"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>529
                     /locus_tag="ACHK1J_005685"
     CDS             <1..>529
                     /locus_tag="ACHK1J_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO7914774.1"
                     /translation="HQTGSDISPEALKSRLTERYFTDFGNAWLNMLNSIQWHEPTSLS
                     GAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSVVDSAKKLLDKNKNGRQF
                     IEQTQGPEGPLDGVFGPLTGLMEGKEGAGGNGNLSFRSWLARVTQMRLKLQQVTSAPD
                     PQAMSQMLARAVFQGK"
BASE COUNT          125 a          134 c          166 g          104 t
ORIGIN      
        1 acatcagaca gggagcgata tctctccgga ggccctgaag agccgcctga ctgaacgcta
       61 ttttaccgat tttggtaatg cctggctgaa catgctcaac agcatacagt ggcatgaacc
      121 cacgtcactt tccggagcca ttgcccagtt gagtctgctc ggtgacgtcc gccagtctcc
      181 gttagttgca ctgatgaaca cgctgaacta ccaggggaaa acggggcaga agggggaggc
      241 actggctgat tcagtcgtgg attcagcgaa aaagctgctc gataagaata aaaatggcag
      301 gcagtttatt gaacagacac agggccctga aggtccgctc gatggggtat ttggaccgct
      361 gacagggctg atggagggga aagagggggc gggtggcaac ggtaatctga gcttccggtc
      421 ctggctcgcc cgtgtaaccc agatgcggct gaaacttcag caggttacca gcgccccgga
      481 tccgcaggcg atgtcgcaga tgctggcccg ggccgtgttt cagggcaaa
//
LOCUS       DAZGBC010000655          524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZGBC010000655 DAZGBC010000000
VERSION     DAZGBC010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.659"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..429)
                     /locus_tag="ACHK1J_005686"
     CDS             complement(34..429)
                     /locus_tag="ACHK1J_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7914775.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYHRDDDMALWSSADLTYSITYEM"
     gene            complement(426..>524)
                     /locus_tag="ACHK1J_005687"
     CDS             complement(426..>524)
                     /locus_tag="ACHK1J_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914776.1"
                     /translation="AFKQNIERIRRERLPEELAYALKQQLRIAIKR"
BASE COUNT          120 a          154 c          119 g          131 t
ORIGIN      
        1 ggcgccaggg gatttggtgt tgccatatca tcctcacatc tcgtatgtaa tggaataagt
       61 cagatctgca gagctccaca atgccatatc gtcatcacga tgatactcat agccctgcgt
      121 aaccatcgtg gtaatcatgc ctgccagtgc agggatcgcc gccatcgccg ggtaaatccg
      181 gctttccatc cactgatcaa gctctgaatc cggtacctgt gccggtaaaa acacctcaat
      241 atgcagtgtg gcccgccagg tatcagcatc cagctcttca ccggtatact ctgcatccgt
      301 cagataaacc gcgaccgccg gaaaatccgc ctcctcaaaa acagcggggc gaccatcaaa
      361 cagcgtcgcc ccggtgtcat gcttctccag tgcatccagt acggctgcac ggatatcagt
      421 atgtttcatc gttttatcgc aatcctcagt tgttgtttca gcgcgtatgc cagttcttct
      481 ggcaggcgtt cacgccggat acgttcaata ttctgtttaa atgc
//
LOCUS       DAZGBC010000656          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZGBC010000656 DAZGBC010000000
VERSION     DAZGBC010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.660"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>523
                     /locus_tag="ACHK1J_005688"
     CDS             <1..>523
                     /locus_tag="ACHK1J_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7914777.1"
                     /translation="VRVARAHGIPAELLYAVSLTETSMSPEAMSGVLKQRRRDFRQPD
                     VARPWPWTINVAGKGYRYATRLEAWQALQVFLKHYPAKRIDVGIAQVNLGWNGHRFPS
                     TWAAFDPYTSLNTAAVILQECRRRHPVSWLQVAGCYHHPAGGAPAARYTAVVRRHLAR
                     LHRDNFLNPSDVHV"
BASE COUNT          100 a          144 c          160 g          119 t
ORIGIN      
        1 gtgcgggtgg cccgtgccca tggtattccc gcagagcttt tgtatgccgt ttccctgacg
       61 gaaacgtcca tgtctccgga agcgatgtcc ggagtactga aacaacggcg tcgtgatttt
      121 cgtcagccgg acgtggcccg accctggccc tggacaataa atgtggcagg aaaagggtac
      181 cgttatgcca cacgtctgga ggcctggcag gcattgcagg tgtttctgaa acattatccg
      241 gcaaaacgta ttgatgtcgg gatagcacag gtcaatctgg gctggaatgg acatcgtttc
      301 ccgtccacat gggcggcatt tgatccttac accagtctga atacggcagc ggtcattctg
      361 caggaatgcc ggcgtcgcca tccggtcagc tggctgcagg tggccgggtg ttatcaccac
      421 ccggcaggtg gtgcgccggc cgcacgttat accgccgtgg tcagacggca tcttgcccgg
      481 ctccaccggg ataatttcct gaatccgtct gatgtgcatg tgg
//
LOCUS       DAZGBC010000657          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZGBC010000657 DAZGBC010000000
VERSION     DAZGBC010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.661"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(156..437)
                     /locus_tag="ACHK1J_005689"
     CDS             complement(156..437)
                     /locus_tag="ACHK1J_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO7914778.1"
                     /translation="MPSVTQTARLEARISTELHAMLKRAAEIQGRTMTDFIISAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
BASE COUNT          127 a          112 c          130 g          152 t
ORIGIN      
        1 atgatcgtta tctacagcga caaaacaggc cgcgatgcgt cgtcgaatat cctgtgtgac
       61 atgctctttt atgtaacggt ccagagcatc agagccacat aaaaatgagg ttctgtcata
      121 tgtgctgtta aatggtgtca gttgaaacat tatatttatt ttgtgtacag catattatta
      181 cggcgtttaa aggcccgttt aagcgcatca ttcggctctg ttggtgacag taatgcctga
      241 gcaaaaattt cctgatctgt ccgggacagt cggattatat cggtttgttc gatagcgcgt
      301 tgggccgcat cctgaacggc agaaatgata aaatcggtca tagtgcgccc ctggatttct
      361 gcagcgcgct tcagcatggc atgtagttct gtgctgatcc ttgcttccag tcgggcagtt
      421 tgtgttacag atggcatgag ttgtacctcc tgaatacact gtacggcaaa ttgccgtatt
      481 gtgcaaatta cactctgaca ccgggacggc agacaggcat t
//
LOCUS       DAZGBC010000658          516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZGBC010000658 DAZGBC010000000
VERSION     DAZGBC010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.662"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..480
                     /locus_tag="ACHK1J_005690"
                     /pseudo
     CDS             80..480
                     /locus_tag="ACHK1J_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          138 a          113 c          129 g          136 t
ORIGIN      
        1 tgatcagagg gtttatcgtg tatacggatc gtggatggca gaaaacgttc aggatcaggt
       61 atccatttta aaccagataa tgagtaagtt taccccatcc atgccccacg cagtgagatc
      121 ggatgtcatt aaaaaagtca ggtcagcagg tcagtatggg aaggcgaaca actcgcagga
      181 atgggtgcgc tgaagttgct ggatgataaa catggcgaac tgaaatcaat gcgtaccgcg
      241 ccaaattatt tacgtcatgg tgtcgccggt cggattttac gccacatttt gcaggtcgcc
      301 catgacagat gccttcatcg cctgagttta gaaacgggta cacaggctgg atttacggcc
      361 tgccatcaac tttatttgaa gcatggtttc gctgattgcg aaccgtttgc tgattatcga
      421 cttgatccac acagtcgatt tttatcattg acgctatgcg aaaataatga attgccatga
      481 gccagacgca gcacattctt gcattcgacg tgctgc
//
LOCUS       DAZGBC010000659          512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZGBC010000659 DAZGBC010000000
VERSION     DAZGBC010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.663"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            110..442
                     /gene="hdeA"
                     /locus_tag="ACHK1J_005691"
     CDS             110..442
                     /gene="hdeA"
                     /locus_tag="ACHK1J_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO7914779.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT          159 a           99 c          112 g          142 t
ORIGIN      
        1 tgattttgat attttccatc aacatgacat atacagaaaa ccaggttata acctcagtgt
       61 cgaaattgat tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt
      121 attaggcgtt attcttggtg gtctgcttct tctgccagtt gtgagcaatg cagcggatgc
      181 gcaaaaagca gctgataaca aaaaaccggt caactcctgg acctgtgaag atttcctggc
      241 tgtggacgaa tccttccagc caactgcagt tggttttgct gaagcgctga acaacaaaga
      301 taaaccagaa gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt
      361 tcaggcttgt actcaggata aacaagccaa ctttaaagat aaagttaaag gcgaatggga
      421 caaaattaag aaagatatgt aattccggga atgcgttaca tcgtacttcc ttgcatattg
      481 aacaggccgg aatatcttct ttaaaagcag ct
//
LOCUS       DAZGBC010000660          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZGBC010000660 DAZGBC010000000
VERSION     DAZGBC010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.664"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>511
                     /locus_tag="ACHK1J_005692"
     CDS             <1..>511
                     /locus_tag="ACHK1J_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914780.1"
                     /translation="INSLYEAANDDTRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWR
                     ALHNPDYRDVLQKTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAW
                     MQDVTTEKLGPLLWHMHPIMFLGCMKKSSGIWPLGKTSERYESGGRGPGVISSGKGDH
                     GGVSYGMYQ"
BASE COUNT          157 a           98 c          128 g          128 t
ORIGIN      
        1 ttatcaactc actctatgag gctgcgaacg atgatacgcg aaccagtcat gcgctggtga
       61 aacataatta tcagcgcctg ctggataaaa ttgatagtgg aagcgaccgt tactctccaa
      121 tggaatactg gagagcactt cataaccccg actatcgtga tgttcttcag aaaacaatcg
      181 ttaaacatcc cagcgactgg tacttcaaaa aagacgatgc aatctggcaa ccatttctga
      241 atgcgctgaa aaaagatgct ccggaatgga agaaatacag cgaagatttt cttgataaga
      301 tggcctggat gcaggatgtg accacggaga aactgggccc tttgttgtgg catatgcatc
      361 cgattatgtt cttaggctgt atgaaaaaat cgtcaggaat atggccactt gggaaaactt
      421 cagagcgtta tgagtctgga ggaagagggc caggtgtaat ttcatctgga aaaggagatc
      481 atggcggtgt gtcatatgga atgtatcaat t
//
LOCUS       DAZGBC010000661          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZGBC010000661 DAZGBC010000000
VERSION     DAZGBC010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.665"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>508
                     /locus_tag="ACHK1J_005693"
     CDS             <1..>508
                     /locus_tag="ACHK1J_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO7914781.1"
                     /translation="EHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPVHLPREIHRLEPEESCCPECGGELDYPGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFAR"
BASE COUNT          123 a          136 c          151 g           98 t
ORIGIN      
        1 agaacatctg cgggctcaga tagaaaaact gcgccggatg ctgttcggta cccgttctga
       61 aaaactgcgt cgtgaagttg aactggctga ggccctgctg aaacaacgtg aacaggacag
      121 cgatcgttac agtgggcggg aagacgatcc tcaggttccc cgccagttgc gacagtcgcg
      181 ccatcgtcgt ccgttaccgg tacaccttcc ccgtgaaata caccgcctgg agccagaaga
      241 aagctgttgc ccggagtgtg gcggtgagct ggattatcca ggggaagtca gcgctgagca
      301 gctggaactg gtgagcagtg ccctgaaagt gatccgcaca gaacgggtaa aaaaagcctg
      361 tacaaaatgt gactgcatcg ttgaagcacc ggcgccgtcc cgcccgatag agcgtggtat
      421 cgcgggcccc ggattacttg cccgcgtgtt aacgggaaaa tactgcgaac atctgccact
      481 gtatcgtcag agtgaaatct ttgcccgc
//
LOCUS       DAZGBC010000662          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZGBC010000662 DAZGBC010000000
VERSION     DAZGBC010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.666"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>508
                     /locus_tag="ACHK1J_005694"
     CDS             <1..>508
                     /locus_tag="ACHK1J_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO7914782.1"
                     /translation="DINGSPWPQSDPPYNAAPGHFDVQFNGNMVSVTPLRAWAAGNVT
                     VYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGPASPVVAVPSEKIALHDA
                     TLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLLVRSRAMLRDEFEQTLASADG
                     TRLWKLPVT"
BASE COUNT          101 a          157 c          139 g          111 t
ORIGIN      
        1 cggacatcaa cggctccccg tggcctcagt cggatccgcc ttataacgcg gcccccggtc
       61 actttgatgt gcagttcaac ggcaatatgg tctccgtcac tcccctgcgc gcctgggctg
      121 cagggaatgt aacggtctac ctcaaaggac tgtctgtacc ggtcattctg aatatcacca
      181 gtggtgaaac cgataccaat accctgagtc aggagatcga cagccgtctg gatctgcgca
      241 ttccccgtca gggacctgcc agtcctgtgg tagccgtacc atcagagaaa attgccctgc
      301 atgatgccac cctgcaggcc tttctggacg gtgttcctcc cgatgatccc accgtgcgtc
      361 ggctgaaatt ctccggaaat gttccgggca ccaccatctg gcagcacggc gatgatctgc
      421 tcgtacgatc gcgggccatg ctgcgggatg aatttgaaca gacgctggca tctgctgacg
      481 gtacgcgtct gtggaaactg ccggtgac
//
LOCUS       DAZGBC010000663          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZGBC010000663 DAZGBC010000000
VERSION     DAZGBC010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.667"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>508
                     /locus_tag="ACHK1J_005695"
     CDS             <1..>508
                     /locus_tag="ACHK1J_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFO7914783.1"
                     /translation="TMKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIY
                     IGLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGV
                     ITLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDV
                     ELSKGNSAL"
BASE COUNT          167 a           77 c          116 g          148 t
ORIGIN      
        1 tacaatgaaa gtgaaaaact ttatatatct tcccttctgc ttgtttattg gaacgtcggt
       61 agccggagct ttaccggaaa ttcctgaacc atttaaatat ggtgtaggag ggatcgagaa
      121 tggtaaaata tatataggac tgggaagtct gggaaataac tggtatatga tagatacaaa
      181 ccagagtgaa aaaaaatgga ctaagatagc acaatggccg acagttccca gagagcaggc
      241 aacagctact attattgatg gtaagatcta tgtatttgga ggaattggaa aagataaaag
      301 tggggtcata accttacaaa aagatgtcta ttcctatgat attgctaaag ataaatggga
      361 aaaattgatg acccgtcccc ctgtatctct tgccggtcat gtttctttta tccataatgg
      421 tcatgcggta tctactggtg gtgtaaatga gaatattttt aatggatact tttcagatgt
      481 cgagttatca aaagggaatt ccgcgctg
//
LOCUS       DAZGBC010000664          501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZGBC010000664 DAZGBC010000000
VERSION     DAZGBC010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.668"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>501
                     /locus_tag="ACHK1J_005696"
     CDS             <1..>501
                     /locus_tag="ACHK1J_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFO7914784.1"
                     /translation="KSNELNTSAFFASRNYNGNNSSNPMELLAHSIIKLICKEAASAT
                     YCGALETLQKMMSECIYQEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNNEVLV
                     TDGEPDVVCLSKQVWENLLIKLKPEIKENAASEVHKSANKGEIEQLVECSKRNEQTLF
                     DNIRKS"
BASE COUNT          185 a           75 c          101 g          140 t
ORIGIN      
        1 taaaagcaac gaacttaaca ctagcgcatt ttttgctagc aggaattata atggaaacaa
       61 ttcttccaac cccatggagc tactagctca tagcattata aaattaattt gtaaggaagc
      121 tgcctcagcg acgtattgcg gtgctcttga aactttacaa aaaatgatgt ctgaatgtat
      181 atatcaagaa ggcaacgcct ttgtcattat gggagctgga gaacaattaa aacgtattaa
      241 atatgatgtt gatgaaaata acttaaaggt attcaacgta cactttgata ataatgaagt
      301 gttagttact gatggtgagc ctgacgtagt atgtttaagc aagcaggtct gggaaaatct
      361 tctcattaaa ttaaaaccgg agatcaagga aaatgcggct tctgaagttc ataaatcagc
      421 gaataaaggt gagattgagc aattagttga atgctctaaa agaaatgaac agaccctttt
      481 cgataatata agaaaaagtg a
//
LOCUS       DAZGBC010000665          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZGBC010000665 DAZGBC010000000
VERSION     DAZGBC010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.669"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(266..466)
                     /locus_tag="ACHK1J_005697"
     CDS             complement(266..466)
                     /locus_tag="ACHK1J_005697"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO7914785.1"
                     /translation="MESGPSCPLCNGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSR
                     GRKRKINFSHKQKSGDVGDKLN"
BASE COUNT          124 a          139 c          112 g          124 t
ORIGIN      
        1 ttatgtgccc ggacagactg aaatcactga tggaaaaagt caacagctaa ccaaaaacaa
       61 aaaagcaccg gcaagccggt gcttcagggg gataccaaat cccggtgttg attctcatct
      121 tctccggaga gagcgactcc cggcacggtt ttcacggagt gcatgtgcct tgtaaccccg
      181 ccgtgtgcat aacgggttac cggacctctc agggctgtca gccccactgt ggtcaacagg
      241 gtgataccag aactcgcccg caattttaat tcagtttgtc tccgacatca ccagattttt
      301 gtttatgaga aaaattaatt tttcttttcc tgcccctgct gtttccctgc tcaccagcaa
      361 ttcccctgca gtcgggataa cgggagcatc cccagaaaaa accgcttttt cccttccgtt
      421 tcaccgtcgg accgttacag agtggacatg aaggacctga ttccactgac agattaaccg
      481 tctggttacg tccggataa
//
LOCUS       DAZGBC010000666          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZGBC010000666 DAZGBC010000000
VERSION     DAZGBC010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.670"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>497
                     /locus_tag="ACHK1J_005698"
     CDS             <1..>497
                     /locus_tag="ACHK1J_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7914786.1"
                     /translation="TPSVWSDRLCSRTCLQFSRKPEQTVPAGMFPDAVLSLLAPFAST
                     VQGGQCTRRLILLIWLCVLTALGISALNNRDLIRQVSTDLQRWNAVPKGHYRPEAESL
                     AALKQDALLLEQWQRQGVPLRYSLGYYPGQRLWLALQQAIDTRVPPPSPEPEPVPEIV
                     RLDSLA"
BASE COUNT           90 a          144 c          148 g          115 t
ORIGIN      
        1 actccgtcag tctggtccga caggctctgt tcccgcacct gtttgcagtt ttcccgaaaa
       61 ccggagcaga cagtgccggc agggatgttc ccggatgcgg ttctgtcgct gctggcacct
      121 tttgcgtcga cggtacaggg aggccagtgt acccgtcgtc tgattttgct gatatggctg
      181 tgtgtcctga ctgcgctggg gatttctgcg cttaacaatc gtgatctgat ccgccaggta
      241 agcaccgatt tacagcgctg gaatgctgtc ccgaaggggc actaccgtcc tgaggcggaa
      301 tcgctggccg cactgaaaca ggatgcgctt ctgcttgagc agtggcagcg tcagggagtg
      361 cctttacgtt acagcctggg ctattacccg ggacaacgtc tgtggctggc cctgcagcag
      421 gctatcgata cccgggttcc accaccatcc cctgaaccag aaccggtgcc ggaaattgta
      481 cgactcgata gcctggc
//
LOCUS       DAZGBC010000667          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZGBC010000667 DAZGBC010000000
VERSION     DAZGBC010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.671"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..236
                     /locus_tag="ACHK1J_005699"
     CDS             <1..236
                     /locus_tag="ACHK1J_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914787.1"
                     /translation="TERPKWVREPSWRFIEDKPMIFISQVEFENNKTMAEHLSSGDVI
                     YMFSGKKEVDGGWELVIKMVIQNKNSVGTSYMN"
     gene            342..497
                     /locus_tag="ACHK1J_005700"
                     /pseudo
     CDS             342..497
                     /locus_tag="ACHK1J_005700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          152 a           77 c          116 g          152 t
ORIGIN      
        1 tgactgaaag gcctaagtgg gttagagaac ctagttggcg atttatcgag gataagccta
       61 tgatttttat ttctcaggtg gagtttgaaa ataataaaac tatggctgaa catctttctt
      121 ctggtgatgt tatttatatg ttttcaggga aaaaagaagt cgatggcggc tgggagttag
      181 taataaaaat ggttatacaa aataaaaact ccgttggaac atcgtatatg aattaaaaac
      241 ttcgagttgt tttagttgag taggtactat gcatcatatc cgtgtagtac ctccataacg
      301 ggtagtatga tagacctatt tagcaagttt gtagtcctga cccgataggg ctaaatggag
      361 gtatcaacct ctatgcttat gcgccgaatc cgctgggttg gatcgatccg cttggattaa
      421 atacggaacc tacgttaccc ccaggaacta ttattaaaga tggtggagta acaatagagc
      481 attattatca tggcagt
//
LOCUS       DAZGBC010000668          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZGBC010000668 DAZGBC010000000
VERSION     DAZGBC010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.672"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          138 a          111 c          110 g          137 t
ORIGIN      
        1 cgacgttcat agaatggcgc aatatgttcg atattaaaat gacctggctt attagtccga
       61 attcagacaa atataaacaa atcccgctca atattaaaaa tactaaccgg taaaagatat
      121 tacttaaaca tgtaaattca ctttctttaa gcaacaaaaa accgccaaaa tcaggcggtt
      181 atttgttgcc ggtttggttc gcggcctctc cagcagattg acttgtgttg catgagcaac
      241 gcaggtgctt cacagcagag caatattcgc cagcaactct ctttttgtca agcaaaagag
      301 agtaattatt gtttatttag cgtattatcg acaccggccc tttccgccgt ctcgcaaacg
      361 ggcgctggct ttaggaaagg atgttccgtg gccgtaaatg caggtgtttc acagcgcttg
      421 ctatcgcggc aatatcgcca gtggtgctgt cgtgatgcgg tcttcgcatg gaccgcacaa
      481 tgaagatacg gtgctt
//
LOCUS       DAZGBC010000669          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZGBC010000669 DAZGBC010000000
VERSION     DAZGBC010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.673"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>494
                     /locus_tag="ACHK1J_005701"
     CDS             <1..>494
                     /locus_tag="ACHK1J_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7914788.1"
                     /translation="LLPFIPANAGILLVPCCRGASAFTTGADGTYSESAGASENSLRW
                     GVGKPLYQDLVSRTKAALAKNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFR
                     TELAGQASQSTGGSASAVPWICGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLM
                     TDEN"
BASE COUNT          114 a          107 c          170 g          103 t
ORIGIN      
        1 aactgctgcc gtttataccg gcgaatgcgg gcattctgct ggttccgtgc tgccgtggtg
       61 cttcggcatt cacaacgggg gcagacggca catacagcga atcagccggt gcatcggaaa
      121 attcactgcg ctggggtgtg ggtaagccgc tgtatcagga tttggtgagc cggactaaag
      181 ccgcactggc gaagaacccg aaaaaccgtc tgcttgcggt ggtgtggatg cagggagaag
      241 gtgatgcggc agtggggacg catgcgcagc atccggggct gtttagtgcg atggtgaatc
      301 agttcagaac ggagcttgcc ggtcaggcat cacagagcac aggaggcagc gcatcagcgg
      361 taccgtggat ttgcggggac acaacgtatt tctggaagca gcgtcatgcg gaagggtatg
      421 catcagtata cggagggtat aagggtaagg agtcgcagaa cattttcttt gtaccgttaa
      481 tgacggatga gaat
//
LOCUS       DAZGBC010000670          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZGBC010000670 DAZGBC010000000
VERSION     DAZGBC010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.674"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          147 a          105 c          104 g          136 t
ORIGIN      
        1 cgattcaaat attttaccaa ggtgtgtttc gtaacaattt tgtgtacaat atgactctac
       61 atatcgacga aaaaaccact tgatttgaca ggaacacatt caaaaaaatt tcttttttca
      121 tttcaggagc gattttcatc tgttacttga agtatctgtg agattaagtg actcccccta
      181 acgtcgtact aaatagtaat tactggccat accgggagca gtattcggcg ctccccacat
      241 gcagcagaat aaccgcatca gcgaagatgt attgtatttt tactatcaca atgtgtacgg
      301 acggtttatg gaaaagaatg aacattagat acgcgatgcg gcggcaggtt acacggttat
      361 cactacgata gccaatactg gttggtgcat taccgccgtg aacaatagga cgccgtactg
      421 ctggaaattt cttaccttta tgactccccg cgccgccgta tcgggaaacg ggaagcaatc
      481 acagggaaag at
//
LOCUS       DAZGBC010000671          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZGBC010000671 DAZGBC010000000
VERSION     DAZGBC010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.675"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>491
                     /locus_tag="ACHK1J_005702"
     CDS             <1..>491
                     /locus_tag="ACHK1J_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO7914789.1"
                     /translation="PWPQSDPPYNAAPGHFDVQFNGNMVSVTPLRAWAAGNVTVYLKG
                     LSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGPASPVVAVPSEKIALHDATLQAF
                     LDGVPPDDPTVRRLKFSGNVPDTTIWQHGDDLLVRSRAMLRDEFEQTLASADGTRLWK
                     LPVT"
BASE COUNT           98 a          149 c          135 g          109 t
ORIGIN      
        1 ccgtggcctc agtcggatcc gccttataac gcggcacccg gtcactttga tgtgcagttc
       61 aacggcaata tggtctccgt cactcccctg cgcgcctggg ctgcagggaa tgtaacggtc
      121 tacctcaaag gactgtctgt accggtcatt ctgaatatca ccagtggtga aaccgacacc
      181 aataccctga gtcaggaaat cgacagccgt ctggatctgc gcattccccg tcagggacct
      241 gccagtcctg tggtagccgt accatcagag aagattgccc tgcatgatgc cactctgcag
      301 gcctttctgg acggtgttcc tcccgatgat cccaccgtgc gtcggctgaa attctccgga
      361 aatgttccgg acaccaccat ctggcagcac ggcgatgatc tgctcgtgcg atcgcgggcc
      421 atgctgcggg atgaatttga acagacgctg gcatctgctg acggtacgcg tctgtggaaa
      481 ctgccggtga c
//
LOCUS       DAZGBC010000672          486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZGBC010000672 DAZGBC010000000
VERSION     DAZGBC010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.676"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..360)
                     /locus_tag="ACHK1J_005703"
     CDS             complement(<1..360)
                     /locus_tag="ACHK1J_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO7914790.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVN"
     gene            complement(369..>486)
                     /locus_tag="ACHK1J_005704"
     CDS             complement(369..>486)
                     /locus_tag="ACHK1J_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914791.1"
                     /translation="VKSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          163 a           87 c           81 g          155 t
ORIGIN      
        1 atttactgga ttcaatgaat caaacttaaa tttaacagct tcagttgcta attgcatttt
       61 actttgaaac caggagacaa gtattgttgt gaacatgcaa accagcaaaa aaggaatgat
      121 cgttttcaac caaccaatcc ccatagcttt aaaatagtcc caaatatctg gtttagcgtt
      181 atgatcaagg atgtattcaa gaatcgacat aatgtgatgc acatcaaaaa catatccgag
      241 atataaagta cccacaagca ttattacaga gacggttaag tctctacttt ttagaatttg
      301 ccctttttta gaagcatcct ggagtttctt ttgtgttggc ttttcagttt tattggccat
      361 gaaacactct atttctcaat gtatagttgc aattgttctg ggaaaaagga taacggcatt
      421 actcgttcag ccagtattgg gccaaaataa ataattaata ttaaaaatgc cagcgcactt
      481 ttgaca
//
LOCUS       DAZGBC010000673          486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZGBC010000673 DAZGBC010000000
VERSION     DAZGBC010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.677"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>486
                     /locus_tag="ACHK1J_005705"
     CDS             <1..>486
                     /locus_tag="ACHK1J_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO7914792.1"
                     /translation="AEIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAVGTLDVPMID
                     FSSVSRRGSLTLLSQGYGVSAKHGGLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHR
                     FSKLITEAAPADINISGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTV
                     GT"
BASE COUNT          149 a           96 c          112 g          129 t
ORIGIN      
        1 cgcagaaata ccatatcaga cttttcgtga ctttgctgaa aataaaggtg tgtttacccc
       61 cggagtgaca ggaattgaaa taaaagataa taatggaaat gcagttggta cgcttgatgt
      121 gccaatgatt gacttcagca gtgtgtctcg gcggggctca ctgaccctgt tgtcgcaggg
      181 atatggtgta tcagcaaaac acggaggcct tggtgatgta aacaatgcca gttttggcta
      241 tgataaaaat aattatactg tagtaaagaa caacaaacat tcgggccttg atttttctct
      301 gcatcggttc agcaaactca tcacagaggc tgcacctgct gatataaata tatccggaca
      361 acttagtgat tcgtcacagt atactgcttt ttacagagca ggcgctggca cacagtatat
      421 taaagaacgc tcaggtaaac agactcatat cccgggaaca tttttaactg gtggaacagt
      481 aggcac
//
LOCUS       DAZGBC010000674          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZGBC010000674 DAZGBC010000000
VERSION     DAZGBC010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.678"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..84)
                     /locus_tag="ACHK1J_005706"
     CDS             complement(<1..84)
                     /locus_tag="ACHK1J_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFO7914793.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVR"
     gene            complement(88..>484)
                     /locus_tag="ACHK1J_005707"
     CDS             complement(88..>484)
                     /locus_tag="ACHK1J_005707"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7914794.1"
                     /translation="LLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
BASE COUNT          131 a          144 c          125 g           84 t
ORIGIN      
        1 gcggacacgc agaccaagcc acagccagat gaccggccag acgatacaca gcgtaaacag
       61 ggtacggagt agtcgtgtca tcataggtta gtagcagaaa tagcgaacaa aatggaagaa
      121 gaccggtgcg gtaaaaatca gcgaatccag ccgatcaagg atgcctccat ggccgggtag
      181 cagcgtacca ctgtctttga cgccgatatc acgcttgatt gccgacatca ccacgtcgcc
      241 gcaaaagcca gtaacgccga taataacccc ggctaacagt gccatcggcc agctcagggg
      301 agtcaacagt ggccccagga ctgttgccgc cagcgccgtc gttgccaccc cacccagcaa
      361 tccctccagg gttttgtttg ggctgacctt cggtatgact tttatacgac caagcgactt
      421 gccccacagg tattgcgcga tgtcgttaaa ttcggtcagg ccgaccaaaa acagcaccaa
      481 cagc
//
LOCUS       DAZGBC010000675          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZGBC010000675 DAZGBC010000000
VERSION     DAZGBC010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.679"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..>482
                     /locus_tag="ACHK1J_005708"
     CDS             34..>482
                     /locus_tag="ACHK1J_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914795.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQVRIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVV"
BASE COUNT          126 a          108 c          151 g           97 t
ORIGIN      
        1 gttaaccggc gacgataaac gcagggtgaa attatggcga taaaagggct tgatcaggcg
       61 attgataatc tgagccgggt tcgtaaaaac gccattcctg ctgcttcagc aatggccatt
      121 aaccgcgtgg ccacaacggc aataaatcag tctgcgtcac aggttgcccg tgagacaaag
      181 gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa gggccacggt aaaaaatccg
      241 caggtcagaa tcaaagttaa ccggggggat ttgcccgtaa tcaggctggg taatgcgcgg
      301 gttgttctgt cccgacgcag gcgacgtaaa aaggggcagc gttcatccct gaaaggtggc
      361 ggcagtgtgc ttgtggtggg taaccgtcgc attcccggcg cgtttattca gcaactgaaa
      421 aatgggcggt ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
      481 gt
//
LOCUS       DAZGBC010000676          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZGBC010000676 DAZGBC010000000
VERSION     DAZGBC010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.680"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..>482
                     /locus_tag="ACHK1J_005709"
     CDS             34..>482
                     /locus_tag="ACHK1J_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914796.1"
                     /translation="MVIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQVRIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVV"
BASE COUNT          128 a          106 c          150 g           98 t
ORIGIN      
        1 gttaaccggc gacgataaac gcagggtgaa attatggtga taaaaggact tgatcaggcg
       61 attgacaatc tgagccgggt tcgtaaaaac gccattcctg ctgcttcagc aatggccatt
      121 aaccgtgtgg ccacaacggc aataaatcag tcggcgtcac aggttgcccg tgagacaaag
      181 gtacgccgga aactggtaaa ggaaagagcc aggctgaaaa gggccacggt taaaaatccg
      241 caggtcagaa tcaaagttaa ccggggggat ttgcccgtaa tcaggctggg taatgcgcgg
      301 gttgttctgt cccgacgaag gcgacgtaaa aaggggcagc gttcatccct gaaaggtggc
      361 ggcagtgtgc ttgtggtggg taaccgtcgc attcccggcg cgtttattca gcaactgaaa
      421 aatgggcggt ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
      481 gt
//
LOCUS       DAZGBC010000677          480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZGBC010000677 DAZGBC010000000
VERSION     DAZGBC010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.681"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>480
                     /locus_tag="ACHK1J_005710"
     CDS             <1..>480
                     /locus_tag="ACHK1J_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO7914797.1"
                     /translation="FTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLTSVK
                     DAQGRETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRV
                     ISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYD"
BASE COUNT          114 a          143 c          150 g           73 t
ORIGIN      
        1 gttcaccgac tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac
       61 ggcggtccac cgcgaggaag gcatcagcct ttaccgccgc tatgacaacc gtggccggtt
      121 aacctcggtg aaagacgcac agggccgtga aacgcggtat gaatacaacg ccgcaggcga
      181 cctgactgcc gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg
      241 aaaggcggtc agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg
      301 acgtgtcatc agcctgacca acgagaacgg cagtcacagc gacttcagct acgatgcgct
      361 ggaccgcctg gtacagcagg gcggctttga cgggcggacg cagcgttatc attatgacct
      421 gaccggaaaa ctcacacaga gtgaggatga gggacttgtc accctctggc actacgacga
//
LOCUS       DAZGBC010000678          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZGBC010000678 DAZGBC010000000
VERSION     DAZGBC010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.682"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..178)
                     /locus_tag="ACHK1J_005711"
     CDS             complement(<1..178)
                     /locus_tag="ACHK1J_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO7914798.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAE"
     gene            complement(195..>476)
                     /locus_tag="ACHK1J_005712"
     CDS             complement(195..>476)
                     /locus_tag="ACHK1J_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO7914799.1"
                     /translation="QNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGF
                     LTHSCLNAFGPSLGPRFCFEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          138 a          102 c           80 g          156 t
ORIGIN      
        1 gttcagcctc tgcagcctta caggcaaaat agctaagtga acacgctttt cgtccctctt
       61 tagtactggc tgatattagt gcccataata ttgctttgtc tgccaaatct aactctctct
      121 gaaattgtct tgcagtaact gcactcgcag aaatagagaa aaaaatacaa ttgttaataa
      181 ccttgccata ttttttagtc cgtaactgtt aatgtgctat ctggctctga aaatattaac
      241 tcatttaatt tttgcatgtc attagatgaa ccagttatac aaccctcgaa acagaatcga
      301 ggtcctaaag acggaccgaa agcgtttaaa caactatgtg ttaaaaatcc atcacgatta
      361 tatgtttctg tatttgctga cggtactaaa cgtataccat taggttttct attggttgct
      421 ccaggaccat taacattcca gctccatacc ccttgaggta ttgggcctct attttg
//
LOCUS       DAZGBC010000679          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZGBC010000679 DAZGBC010000000
VERSION     DAZGBC010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.683"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>476
                     /locus_tag="ACHK1J_005713"
     rRNA            <1..>476
                     /locus_tag="ACHK1J_005713"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZGBC010000680          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZGBC010000680 DAZGBC010000000
VERSION     DAZGBC010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.684"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>473
                     /locus_tag="ACHK1J_005714"
     CDS             <1..>473
                     /locus_tag="ACHK1J_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO7914800.1"
                     /translation="IPVHDTIARVVYCISPAKFHECFINWMRDCHSSDDKDVIAIDGK
                     TLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPGLLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYA"
BASE COUNT          156 a           92 c          104 g          121 t
ORIGIN      
        1 attcctgttc acgataccat tgccagagtt gtatactgta tcagtcccgc aaaatttcac
       61 gagtgcttta ttaactggat gcgtgactgc cattcttcag atgataaaga cgtcatcgca
      121 attgatggaa aaacactccg gcattcttat gacaagagtc gccgcaaggg agcgattcat
      181 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcag gacggatgag
      241 aaatccaatg agattacagc tatccctgga cttcttaaca tgctggatat taaaggaaaa
      301 attatcacaa ctgatgctat gggttgccag aaagatatcg cagagaagat acaaaaacag
      361 ggcggtgatt atttattcgc tgtaaaagga aatcaggggc ggcttaataa agcctttgag
      421 gaaaaatttc cactgaaaga attaaataat ccagagcatg acagttacgc aat
//
LOCUS       DAZGBC010000681          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZGBC010000681 DAZGBC010000000
VERSION     DAZGBC010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.685"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHK1J_005715"
     CDS             complement(<1..186)
                     /locus_tag="ACHK1J_005715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HFO7914801.1"
                     /translation="MYMLGGMSDTSSNRITFERDSQYRITGVSHTDGIRLKLTYHASG
                     YLKAIHRTGNGIQTQGKI"
     gene            complement(230..>473)
                     /locus_tag="ACHK1J_005716"
     CDS             complement(230..>473)
                     /locus_tag="ACHK1J_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO7914802.1"
                     /translation="FIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGHITLLYGE
                     NNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
BASE COUNT          117 a          119 c           98 g          139 t
ORIGIN      
        1 gatctttccc tgcgtctgta tgccgttacc cgtgcggtga atggctttca ggtagccgct
       61 ggcgtggtac gtcagtttaa gccggatccc gtcggtgtga ctgacacctg tgatccggta
      121 ctggctgtcg cgctcaaacg tgatgcggtt gctggaggtg tcactcatcc cgcccagcat
      181 atacatccgg tcgtcggggt tgtgtgccag gtgtcatatc aagttggcct tacggaattt
      241 caactgcctc cttgccatta atcaatatat caattgatga agataaatta tccccatact
      301 tccaaagata atcatccaag ccgccatata tcacattatt ttccccatat aataatgtga
      361 tatgaccatt actgcactcg ccaacaggaa tcatattgta accagctctc tcaatataat
      421 cattgactct ttctataaaa atgcgattag aggccctttc tggatctata aaa
//
LOCUS       DAZGBC010000682          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZGBC010000682 DAZGBC010000000
VERSION     DAZGBC010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.686"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>471
                     /locus_tag="ACHK1J_005717"
     CDS             <1..>471
                     /locus_tag="ACHK1J_005717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO7914803.1"
                     /translation="AQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDI
                     HRFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLA
                     ENGFYRVSPSRAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYS"
BASE COUNT           88 a          126 c          155 g          102 t
ORIGIN      
        1 ggcacagatg cagggggaga ttgtggcgct ggtccacagt catcccggtg gactgccctg
       61 gctgagcgag gcggaccggc ggctgcagat aaagagtgcc ctgccctggt ggctggtctg
      121 ccggggtgac attcaccggt tccgctgtgt gccgcacctg accggacggc gctttgaaca
      181 cggtgtgacg gactgttaca ccctgttccg ggatgcttac catctggcgg ggataacgct
      241 gccggatttt gtgcgtgagg atgactggtg gcgcaacggt cagaacctgt acctggacaa
      301 cctggcggaa aacggctttt accgggtgtc tccgtcccgt gcacaggcag gcgatattct
      361 gctgtgctgc tttggcgcat cggtgccgaa tcatgccgcc atttactgtg gcaacggtga
      421 actgcttcac catatacctg aacaactgag taaacgggag aggtattcag a
//
LOCUS       DAZGBC010000683          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZGBC010000683 DAZGBC010000000
VERSION     DAZGBC010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.687"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(96..>469)
                     /locus_tag="ACHK1J_005718"
     CDS             complement(96..>469)
                     /locus_tag="ACHK1J_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7914804.1"
                     /translation="CLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLRGH
                     LAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIHPR
                     WYISVTDAADLFYLLLWLGEG"
BASE COUNT          120 a          165 c           98 g           86 t
ORIGIN      
        1 ccacagctcc cgccagaaac agcacaggtt cactggcaat ttgatctgac agcgatggat
       61 acagtggtcc cggaaaatcg atatcgctcc tcatatcacc cctctcccag ccacaacagc
      121 agatagaaca aatccgccgc atcagtcact gatatatacc agcggggatg aatatactcc
      181 tcctccgacg gccagctctc atgcccactc acagtaagca gaatatgcac atactgcccc
      241 tgctgacctt ccagcatcac cgccacactg ctgttcagac ccaccctctg tggctggcaa
      301 cggatacact ccacccgccc cagttgcgcc gccagatgcc cgcgaagacg ttcaagatta
      361 tcccgtgtgg tgcaacttat ccccggcgca ccctgtggcg gcagaacaaa cacacttccg
      421 gcagggtgaa cagcagacac cacttccggt tccggatact tcagacaca
//
LOCUS       DAZGBC010000684          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZGBC010000684 DAZGBC010000000
VERSION     DAZGBC010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.688"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>459)
                     /locus_tag="ACHK1J_005719"
     CDS             complement(<1..>459)
                     /locus_tag="ACHK1J_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7914805.1"
                     /translation="QYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSK
                     AGIDKVAIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDND
                     CLWKKADTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEIDIV"
BASE COUNT          109 a          126 c          128 g           96 t
ORIGIN      
        1 cacaatgtcg atttcaaacc aggtactggc cgcagtgagc ccgatggtgt cgaggccgcg
       61 cagttcaaca aacataaact tcggacggaa gctgaaatac tccagcagca actggtaacc
      121 actgaaggct gtatcggctt tcttccacag gcagtcattg tcgtcaaagc ccatgggtct
      181 gcaccagccg tcaaactgat gacgtccggt atatgtcccg gcatgacgcg catacatggc
      241 atgtacacgc cgggtcattg ccagatgcag agcagcgctt accggcgcct ctgcattaag
      301 aaaaatggcg actttgtcga tcccggcttt actccagtcc actttttcag ggcattcgaa
      361 gcgcaggcgg atggcagagc ggccatcgga ttcagtatgg agacgggcat ctgcaaggtg
      421 cagcggttgt agcaacacat cgcgggtcgt ccggtactg
//
LOCUS       DAZGBC010000685          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZGBC010000685 DAZGBC010000000
VERSION     DAZGBC010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.689"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..360
                     /gene="ssb"
                     /locus_tag="ACHK1J_005720"
     CDS             <1..360
                     /gene="ssb"
                     /locus_tag="ACHK1J_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7914806.1"
                     /translation="IAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYSTEVVVSVNGT
                     LQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGMPPMDFDDDI
                     PFMGRGYGVERRIIHVI"
BASE COUNT          114 a          104 c          130 g          107 t
ORIGIN      
        1 attgccggtg aataccttcg taaaggttca caggtgtata ttgaaggtca gttaagaacc
       61 cggaagtggt tcgatcagca gaatggtgtt gatcgttatt ccacggaagt tgttgtgagt
      121 gtcaatggta ccctgcagat gctggggtct ccgcgtcagc agcagggtgg tggaaatgtt
      181 tcatctgctc ctgctcaggg aggatgggga caaccaccac aaaatgcaca acaatcaacg
      241 ggaaaaaacg gtcaggcttc ctcaggaagc gggggaatgc cgccgatgga ctttgacgat
      301 gatatcccct ttatggggcg tgggtatggc gttgaacgac ggattatcca tgtcatctga
      361 tgttaaccac ggggcttacc gccccacttt taattcaagg aggcggttgt gaatgaaaac
      421 acacccacta cccccacgcc acatgatgcg gcgtt
//
LOCUS       DAZGBC010000686          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZGBC010000686 DAZGBC010000000
VERSION     DAZGBC010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.690"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>455
                     /locus_tag="ACHK1J_005721"
     CDS             <1..>455
                     /locus_tag="ACHK1J_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO7914807.1"
                     /translation="RCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAML
                     RKTGLGFICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQ
                     AEIGRDVESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFC"
BASE COUNT          118 a          121 c          118 g           98 t
ORIGIN      
        1 cgctgccacc tgatacaggg aacatcggca acagaagaaa aagccatttc cgtcaccatg
       61 cgggtgatcc cgtcagatat tccggacatc ctcactatga atattgaacc ggatctgctg
      121 gatgccatgc tcagaaaaac cggcctgggc ttcatctgcg gagaaaccgg ctccgggaaa
      181 tccacactgg cggcggcact gtatcgccac attctgaaaa atttccccga tcgcaaaata
      241 gtgacctatg aagatccggt ggaatacatt ctgggcggag agaatgacct tctgcctccc
      301 catcaggccg aaatcgggcg cgatgtggaa agttttgctg caggacttcg ctctgccgtt
      361 cgccgtaatc cggacatcat tggtatcggt gaaatcaggg attatgaaac agcagatgcc
      421 gcagtccagg cgggtaatac cggacacttt tgtat
//
LOCUS       DAZGBC010000687          449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZGBC010000687 DAZGBC010000000
VERSION     DAZGBC010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.691"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>449
                     /locus_tag="ACHK1J_005722"
     CDS             <1..>449
                     /locus_tag="ACHK1J_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO7914808.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPLIDGTEVAIVFDGGDPDRPYIAYALHDSEHPDHVTSDNHTRNVWR"
BASE COUNT           93 a          122 c          131 g          103 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgcttat cgacggaacg gaagtggcca ttgtatttga cggcggagat
      361 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
      421 agtgacaatc atacgcggaa cgtgtggcg
//
LOCUS       DAZGBC010000688          449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZGBC010000688 DAZGBC010000000
VERSION     DAZGBC010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.692"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..237
                     /locus_tag="ACHK1J_005723"
     CDS             <1..237
                     /locus_tag="ACHK1J_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO7914809.1"
                     /translation="VTSLQPLIESFTHPNAIVLDPFAGSGSTCVAALQSGRRYIGIEL
                     LEQYHRAGQQRLAAVQRAMQQGAANDDWFMPEAA"
     gene            238..>449
                     /locus_tag="ACHK1J_005724"
     CDS             238..>449
                     /locus_tag="ACHK1J_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914810.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDAC"
BASE COUNT           97 a          131 c          136 g           85 t
ORIGIN      
        1 gttaccagcc tgcaaccgct gattgagagc ttcacacacc cgaacgcaat tgtgctggac
       61 ccgtttgcag gcagcggctc aacctgcgtc gccgccctcc agtccggacg ccggtatatc
      121 ggtatcgagc tgcttgagca gtatcaccgt gccgggcagc aacgccttgc cgccgtgcaa
      181 cgggccatgc agcagggggc cgcgaatgat gactggttta tgccggaggc tgcgtaaatg
      241 aactatgccg gacacgaaaa actgcgcgcc gaagtggcgg aggtggccaa tgccatgtgc
      301 gacctgcgta caaccatgaa tgagatggag cagcggtaca gctttaatgc cgacaccctg
      361 ccggaacgtc tggtgcgtca gacgctgttt cgcgcaaacc gcctcctgat ggaggcatat
      421 accgaaattc tggaactgga tgcgtgctt
//
LOCUS       DAZGBC010000689          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZGBC010000689 DAZGBC010000000
VERSION     DAZGBC010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.693"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(105..>443)
                     /locus_tag="ACHK1J_005725"
     CDS             complement(105..>443)
                     /locus_tag="ACHK1J_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFO7914811.1"
                     /translation="GYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAP
                     IAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGL
                     IYRTLLEKRN"
BASE COUNT          129 a          116 c          110 g           88 t
ORIGIN      
        1 tgcgccagtg actatatgtg gcttaacaca tcaaggagcg caacaggaag atgaatcaat
       61 atatttaaaa aaggcctgac attgcgccag gccttctgcg ttaattaatt acgtttttcc
      121 agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
      181 aaccacagtt gttctaatgc ccagccgccc tggaagatgg caaccgcggt gctgcgcgcc
      241 gggttaacag aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga
      301 ccaatagcga tcggcgcaaa acctgccgga gcgaatttgt cggttgcgcc gtggatcacc
      361 aacaggaaac ctgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg
      421 cctggtgaat gctcgccata acc
//
LOCUS       DAZGBC010000690          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZGBC010000690 DAZGBC010000000
VERSION     DAZGBC010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.694"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..86)
                     /locus_tag="ACHK1J_005726"
     CDS             complement(<1..86)
                     /locus_tag="ACHK1J_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914812.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASV"
     gene            104..295
                     /gene="yfgG"
                     /locus_tag="ACHK1J_005727"
     CDS             104..295
                     /gene="yfgG"
                     /locus_tag="ACHK1J_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO7914813.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          103 a          112 c           82 g          133 t
ORIGIN      
        1 tccactgacg cttttttaca taacgaagaa ttgaccattt tgtcctgttg tgccttaatg
       61 taagtaccgt ccacagcgtg ggacatactt caaggaacct tttgtgagtc aggcaaccag
      121 tatgcgaaaa cgacaccgat ttaacagtcg catgacccgt atcgtactgc tcatcagctt
      181 tatcttcttc tttggccgtt ttatctactc gtccgtcggt gcctggcagc accatcagag
      241 caaaaaagaa gctcagcaat ccacactctc cgtcgaatca ccggtacaac gttagcggtt
      301 accttctcca ctttcacaga acataacggc gcttcgctgt cggatgcttt tgctctttgg
      361 aattatcaaa gcggcagata ttctttcatc ttaaatttta cgtctttgtc ctgactgatg
      421 tttatcctgt
//
LOCUS       DAZGBC010000691          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZGBC010000691 DAZGBC010000000
VERSION     DAZGBC010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.695"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(7..>428)
                     /locus_tag="ACHK1J_005728"
     CDS             complement(7..>428)
                     /locus_tag="ACHK1J_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914814.1"
                     /translation="SALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFISMMILSRVYD
                     FLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETETISERLIR
                     EADTREYELWRPVRRFFSIIFLIILLVCIHSLITTIK"
BASE COUNT          147 a           76 c           81 g          124 t
ORIGIN      
        1 taacttttat tttatagttg ttatcagtga atgaatgcaa actaatagaa taatgagaaa
       61 aataatggag aaaaagcgac ggaccggacg ccatagttca tattctcgtg tatcagcctc
      121 acgtatcaac ctttctgaaa tggtttcggt ttcttttcct cgcaccagaa tatgtctgta
      181 atatttcctc atgacatata ccgaaaactg aaaccagatt aatatggatg gacacacaac
      241 acataaactt ctgatgatat cacccgggaa agtatcttgg gcgggtaaaa gaaagtcata
      301 tactctggat aaaatcatca tcgagataaa tgagacgcca gcacctatcc agtattcgat
      361 atgagaagat gctgcattat atgcgtcagt aaaatttttc tttcttaatg aaggtggaag
      421 tgctgaaa
//
LOCUS       DAZGBC010000692          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZGBC010000692 DAZGBC010000000
VERSION     DAZGBC010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.696"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHK1J_005729"
     CDS             <1..207
                     /locus_tag="ACHK1J_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7914815.1"
                     /translation="APKARTPTAASTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVG
                     SRVVSQEISTADEGDGGQVVVIGR"
BASE COUNT          100 a           93 c          142 g           90 t
ORIGIN      
        1 gcaccgaaag ccaggacgcc cacggcagcc agtacagata acggcaaaca gaacacgtat
       61 ttctcgtcac tggataacat ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa
      121 atgcgtgtgg ggtctcgtgt ggtttctcag gagatcagca cggcagatga aggggacggt
      181 ggtcaggttg tggtgattgg ccgctgatgc aaaatgtttt atgtgaaacc gcctgcgggc
      241 ggttttgtcg tttatggagc gtgacgaatg ggtaaaggca gcagtaaggg gcataccccg
      301 cgcgaagcga aggacaacct gaagtccacg cagctgctga gtgtgatcga tgccatcagc
      361 gaagggccgg ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg
      421 gtgct
//
LOCUS       DAZGBC010000693          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZGBC010000693 DAZGBC010000000
VERSION     DAZGBC010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.697"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>423
                     /locus_tag="ACHK1J_005730"
     CDS             <1..>423
                     /locus_tag="ACHK1J_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914816.1"
                     /translation="HIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKD
                     IFDGVYEFIRNIRKNSTPGKIKLIATINTCKETCKHLLYLSNEDKEKIISDLDKIQVA
                     CYYLTILLAFERRTSLTSTLATLYKMLISEREVSEYECQ"
BASE COUNT          158 a           75 c           67 g          123 t
ORIGIN      
        1 catattcatt cttttctcac tcagataccg gataatgagg atgatatata tcatgctgca
       61 aggacatatc tgcgaaatta cgttaagtta tgtttatccg gatacacacc agcgaatgcg
      121 catttcaaag atatctttga tggcgtatat gaattcattc gtaatattcg caaaaacagt
      181 acaccaggaa aaataaaact tatcgcaact atcaacacat gcaaagaaac ctgtaaacat
      241 ctgctttatt taagtaatga agacaaggaa aaaataattt ctgacttaga taaaattcaa
      301 gttgcatgtt attatctcac tatattactg gctttcgaaa gacgaacttc attaacaagc
      361 accctggcaa ctttatataa aatgctgata agcgaaagag aagtttcaga atatgaatgc
      421 cag
//
LOCUS       DAZGBC010000694          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZGBC010000694 DAZGBC010000000
VERSION     DAZGBC010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.698"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>422
                     /locus_tag="ACHK1J_005731"
     CDS             <1..>422
                     /locus_tag="ACHK1J_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7914817.1"
                     /translation="LEQLSDKPESLSMRITATNGQLPRRALESTLLDRVVKTGKVPVR
                     VLNLTAPTMPLYPPANDRFHWRVMSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRR
                     RLEAIVAVKHTLIRRFEKGFMLRGVDIEVTLNMDNFAG"
BASE COUNT           92 a          120 c          125 g           85 t
ORIGIN      
        1 ctggagcagt tgtctgataa accggagtcc ctctcgatgc gaatcaccgc gaccaacggg
       61 cagcttccgc gcagggcgct ggaaagtacg ctcctggaca gggtggtcaa aaccggcaaa
      121 gtccccgtca gagtcctgaa cctgaccgca ccgacgatgc cactgtatcc cccggcaaat
      181 gaccggtttc actggcgggt gatgagtcac ctcggttcaa actttctcag catgatggac
      241 aatccggagg tgctgcgggg gacgctggcg ctctatgact ggaccgacga tgaaatgaac
      301 cgccgccggc ttgaagccat cgttgccgtg aagcacaccc tgatccgccg ttttgaaaag
      361 ggctttatgc tccgtggcgt ggacattgag gtcacgctga atatggataa ttttgccggc
      421 ga
//
LOCUS       DAZGBC010000695          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZGBC010000695 DAZGBC010000000
VERSION     DAZGBC010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.699"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>420)
                     /locus_tag="ACHK1J_005732"
     CDS             complement(<1..>420)
                     /locus_tag="ACHK1J_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914818.1"
                     /translation="YNNGYPTTYGNIIHLKGMTAVGEGELLIGWSGTSGAHAPAFIRS
                     RRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPK
                     LAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIK"
BASE COUNT           99 a          113 c          110 g           98 t
ORIGIN      
        1 atttaatgcc gtcctgttcc tgagacaata cggcccgacc actggcaggt ttgcccttaa
       61 tcgtccagcc acgcatatca gggatcacgc ctgacggata agcggctgca agtttcgggt
      121 atgcagattt gtcaaaagtc tgcccctgca tcagggcata accagacgga acggtatctg
      181 atggccacgg gattggtgca ccgactggat aaaactctgc aggaggatga gccgaggtgt
      241 aaagctgcgc ccacggcgac cagtttgcgt cggtcgtatc ccgtcgtgaa cgaataaatg
      301 ccggagcatg agcaccgctt gtaccactcc agccgatgag taactcacct tcgccaacgg
      361 ctgtcatccc tttcaggtga atgatatttc catacgttgt tggatatccg ttgttataca
//
LOCUS       DAZGBC010000696          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZGBC010000696 DAZGBC010000000
VERSION     DAZGBC010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.700"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..268
                     /locus_tag="ACHK1J_005733"
     CDS             <1..268
                     /locus_tag="ACHK1J_005733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914819.1"
                     /translation="GQKARKVIREVLQARKVLKVNEEKPDHRDRWEHEVSVGRLAPEV
                     NLVLQVREANEERPDLRDLVESQVRQAALQMWLMRRRHRRELCS"
     gene            259..>420
                     /locus_tag="ACHK1J_005734"
     CDS             259..>420
                     /locus_tag="ACHK1J_005734"
                     /inference="COORDINATES: protein motif:HMM:NF015371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914820.1"
                     /translation="MQLSSATDSDDETKAATPKAVKAAMDKADGCLEKAKNGDDIPDK
                     VKFLNTVGAA"
BASE COUNT          118 a           98 c          157 g           47 t
ORIGIN      
        1 gggccaaaag gcgagaaagg tgatccggga ggtcctgcag gcccgcaagg tcctaaaggt
       61 gaacgaggag aagccggacc acagggaccg atgggagcac gaggtgagcg tggggagact
      121 ggcccccgag gtgaacctgg tcctgcaggt ccgagaggcg aacgaggaga gaccggacct
      181 cagggacctc gtggagagcc aggtccggca ggcagcgctg caaatgtggc tgatgcgacg
      241 acggcacaga agggaattgt gcagttaagc agcgcaacgg acagtgatga tgaaacgaag
      301 gcagccaccc cgaaagcggt gaaagcggca atggacaaag cggacggatg cctggagaaa
      361 gcgaaaaacg gtgacgatat cccggataag gtgaagtttc tgaacaccgt gggagcagcc
//
LOCUS       DAZGBC010000697          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZGBC010000697 DAZGBC010000000
VERSION     DAZGBC010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.701"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(42..>420)
                     /locus_tag="ACHK1J_005735"
     CDS             complement(42..>420)
                     /locus_tag="ACHK1J_005735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO7914821.1"
                     /translation="YVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTDTSLQLPI
                     ESVFLVFLSGILQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISF
                     IQGLGVFITILCVYILNKKYAIE"
BASE COUNT          177 a           76 c           78 g           89 t
ORIGIN      
        1 gtcagggtgt accctaccgc gataacgcaa ccctaataaa gtcattcgat ggcatatttc
       61 ttattgagaa tatatacgca taaaatagtt ataaaaacgc ctagtccctg aataaatgat
      121 atatcttcct gcaggaagaa ccaggacaac agcaatgcta ccagaggaat aaaatagagc
      181 attgctcctg ctatgtgaac gggaatccag cgtagggaat aaagatagaa acaaaaagcc
      241 aaaaaatatt gtaaaattcc tgacaaaaac acaagaaata cagattctat tggcagttgc
      301 agggatgtgt cagttagaac aataaaaaaa caagcaagaa taaaagagaa agtcaactgc
      361 ccagttaaca acgtaacagg ctcaaaattc acaataagtc gtgatgagat aacaacataa
//
LOCUS       DAZGBC010000698          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZGBC010000698 DAZGBC010000000
VERSION     DAZGBC010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.702"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(241..>419)
                     /locus_tag="ACHK1J_005736"
     CDS             complement(241..>419)
                     /locus_tag="ACHK1J_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914822.1"
                     /translation="LTLNAAVNNLLNKDYSDVSLYSAGKSSLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          108 a          100 c           87 g          124 t
ORIGIN      
        1 acgtcactct ctctgtaaac taatttttac cccacgtcct tcacggtgcg cttacactct
       61 ggacaagtat gtaaatgata agttaaaaat aatccgtgca tttcctgtga cgatattagt
      121 tgttggtaac gaaatctcat tagcggatcg gaaatgcact taacctaata cgttgccact
      181 gttccgccag gagttgcagt ggcaacgtat tctaccgtca gtgatagcgt tttgttatta
      241 tcagaactga tagttcagcg acatccagta atttcgctca ggtatcacat atcctgttgt
      301 tgatgatccc gtctggaagt aatcaccggc atacagcgaa ctcttaccgg cactgtacag
      361 gctcacgtca ctgtaatcct tgttgagcag gttattcacc gcagcattca gcgtcaggg
//
LOCUS       DAZGBC010000699          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZGBC010000699 DAZGBC010000000
VERSION     DAZGBC010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.703"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            201..>418
                     /locus_tag="ACHK1J_005737"
     CDS             201..>418
                     /locus_tag="ACHK1J_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914823.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVCLYIG"
BASE COUNT          103 a           58 c          110 g          147 t
ORIGIN      
        1 gttatttcga tcgttatgat cgagtgtgtt tcaagagtgc ttttaactct gttttaagcg
       61 tgaatgaaaa ggattcggac aggacgtgat ttagcatgcc gctggtctgg cattatgctg
      121 cagagcagac ggagtcagga gattgaaagt atttcgttat ataatctttt ccttttcggg
      181 atgaaatcaa cgaggttcgt atgaaaatta aacagcataa agaaaaactg gagattataa
      241 gggatattat tcgagagacg ctgttgggta atgctgctct tattgttctg gcagccatgg
      301 ttccggttgt actggtgaaa ccatgggatt cgatgacatt tatttttatg ggggccgtgt
      361 gtgtttcttt tgcagtcatt ctgttctggc tggtttatat agtttgtttg tatatagg
//
LOCUS       DAZGBC010000700          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZGBC010000700 DAZGBC010000000
VERSION     DAZGBC010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.704"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..282
                     /locus_tag="ACHK1J_005738"
     CDS             <1..282
                     /locus_tag="ACHK1J_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914824.1"
                     /translation="SAFIISNYPPFKNKVLLILSATSPVQLVILSFDQCLNVEVLFDS
                     MSPVFAKYREDISATLPLWKVILTKNDIELYEYHRTNPYSLNKLLSMSK"
BASE COUNT          137 a           69 c           73 g          138 t
ORIGIN      
        1 tcagcattta tcatatctaa ttacccacca tttaaaaaca aagttctgtt aattttatcc
       61 gctactagtc ccgttcagtt ggttatattg tcttttgatc aatgtcttaa tgtggaggtg
      121 ttatttgatt caatgtctcc agttttcgct aagtatagag aagatattag tgcaacatta
      181 ccattatgga aggttatttt gacaaaaaat gatatagaat tatatgagta ccatagaaca
      241 aatccatact cacttaacaa attattgagc atgtcaaaat aacggtcaag tattcaacgt
      301 tgtaatatta ttccttttgc tgagtgtctc gaacggctaa agttgacgta aaatgccacg
      361 ttttgtgggc aaaccgaatc ggttaaaaag catggtccgg gaaaagctaa acatcag
//
LOCUS       DAZGBC010000701          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZGBC010000701 DAZGBC010000000
VERSION     DAZGBC010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.705"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..267
                     /locus_tag="ACHK1J_005739"
     CDS             <1..267
                     /locus_tag="ACHK1J_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO7914825.1"
                     /translation="DSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWIT
                     RRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            228..>415
                     /locus_tag="ACHK1J_005740"
     CDS             228..>415
                     /locus_tag="ACHK1J_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7914826.1"
                     /translation="MQRQPATTLLALLIGCTVIPSTHAYPVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTA"
BASE COUNT          102 a          140 c          106 g           67 t
ORIGIN      
        1 gatagccccg gcacaccggc actgacggcg ggagactggc cggtaatgga aaccgatacc
       61 ccgacgctga aacgcgtgaa gcgtcgtacc ttcacgctca tcgataaccg caaccgtcag
      121 ggatggatca cccgtcgctt ccagtgcttc ccgctggctc cgctgccgga atcacacagt
      181 caccgcgtac cagacatcct gtatcccctg aaaacggaga aaaaagaatg caaagacaac
      241 cagcaaccac actgcttgca ctcctgatag gctgtacggt tatcccgtcc acccacgcct
      301 atcctgtgac cgtagtggcc agccagcctg tcaccacaca aatcatgccg gagctggccg
      361 ccattcaggg cgtgatggca gagatggtcc gtatgcagac agccaccggc acagc
//
LOCUS       DAZGBC010000702          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZGBC010000702 DAZGBC010000000
VERSION     DAZGBC010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.706"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>415
                     /locus_tag="ACHK1J_005741"
     CDS             <1..>415
                     /locus_tag="ACHK1J_005741"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO7914827.1"
                     /translation="LIFMKNGKTTNNQNASYAPDIRNGSFVLIDGWEGERITTNNLFD
                     EFKNRTTGGDSGSSLFVYDNEKNKWVILGTLFGEYHYSNGQIRSAFNKYDQSLVNKLK
                     DYFTQQINLSGKSAVIKNDNITINSSSDSIKVVNQL"
BASE COUNT          144 a           69 c           77 g          125 t
ORIGIN      
        1 gtcttatttt tatgaagaat ggaaaaacaa caaataacca gaatgcaagt tatgctcctg
       61 atatcagaaa tggttctttt gtactgattg atggctggga aggcgaacgc atcaccacca
      121 ataatctttt tgatgaattt aaaaacagaa ccactggtgg tgacagtggg tcttctcttt
      181 ttgtatacga taatgaaaag aataaatggg ttattcttgg tacattattt ggtgaatatc
      241 attacagcaa tgggcagatc cgttctgcat tcaacaaata tgaccagtct ctggtgaaca
      301 agttgaaaga ttattttact caacagataa acctgtcagg taaaagcgca gtcataaaaa
      361 atgacaatat aactatcaac tcctcatctg acagtattaa agttgttaac cagct
//
LOCUS       DAZGBC010000703          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZGBC010000703 DAZGBC010000000
VERSION     DAZGBC010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.707"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>412
                     /locus_tag="ACHK1J_005742"
     CDS             <1..>412
                     /locus_tag="ACHK1J_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914828.1"
                     /translation="PASLAAQNTNVVEVDESSQGYDVHGDNWKSIEIGTTQEVKASPD
                     KNINFDHNSPVAEGRNSIAIGTSAEAGKEMTDKMAGEAIAIGAKAKATKEQSIAIGGD
                     TKATGWGAIVIGGDDLTPLHGKTYGREKIENGYLA"
BASE COUNT          135 a           69 c          112 g           96 t
ORIGIN      
        1 tccctgcatc actggcagca caaaacacta atgtagttga agttgatgaa agcagtcagg
       61 ggtatgatgt gcatggagat aactggaaga gcattgaaat agggacaacg caggaagtta
      121 aagcatcccc cgataaaaat attaatttcg atcataattc tcctgttgca gaaggacgta
      181 actctatcgc gattggtacc agtgcagagg ctggtaaaga aatgacggat aaaatggcag
      241 gggaagccat agctattggt gccaaggcga aagcaactaa agaacagtca attgcaatag
      301 gtggcgatac caaagctacc ggatgggggg ctattgttat tggtggggat gatttaactc
      361 ctttgcatgg taagacttat ggtcgggaaa aaatagagaa tggctatctt gc
//
LOCUS       DAZGBC010000704          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZGBC010000704 DAZGBC010000000
VERSION     DAZGBC010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.708"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..317
                     /locus_tag="ACHK1J_005743"
     CDS             <1..317
                     /locus_tag="ACHK1J_005743"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7914829.1"
                     /translation="SAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
BASE COUNT          121 a           89 c           96 g          104 t
ORIGIN      
        1 tatcagcggc cagtatatta ttaactgact tacctgagca tttgagaaaa aagcttccgc
       61 gatatcccac tattccggct attcgtatgg gacgactggc agtaagtctg tcatcaaaag
      121 gaacggggct tgggggagca cttttagctg atgcgctata tcgtgcatac acatctgaaa
      181 ttgcagcata tgcgcttatt gttgatgcaa aagatgacac ggcagtcgca ttttacaaac
      241 attatggctt tatggcgttc ccggagtccg gaagaacttt attcatgcca ttggcatcat
      301 tatccacaat aaaataaaca gaataaatca gggaaatacg gtgcttgtgg caacgtcagc
      361 ccacgaagtt aaatgttaac gggcaacacg gcggcaggaa atctgccgcc
//
LOCUS       DAZGBC010000705          408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZGBC010000705 DAZGBC010000000
VERSION     DAZGBC010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.709"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHK1J_005744"
     CDS             complement(<1..242)
                     /locus_tag="ACHK1J_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914830.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFEL"
BASE COUNT          125 a           91 c           96 g           96 t
ORIGIN      
        1 agttcaaacc aggtcaccac acctttcacc cggcgttgaa catcatcacc ctgccatacc
       61 gtcaggtagg ccattttgtc cagtacctgc gcaaattcaa gggagagaaa ctgctggctg
      121 accagggaga gatcgaggga aaagagtgaa gagagtgact gggtgagatg aaaggaaacc
      181 accgcaaaag cgtccggtgg caggccgtcc acttccagtg tgaaacgtaa tccggttgac
      241 atagcaacct cctgtataag aaaaactaac ctgcactctg ttgtggaaca gcgtgtcagg
      301 gaataatatt aatgagctga tgcccgatat taccggtaat atatcatgta cgtttctgcg
      361 attttattaa taaaaattca attcacatgg ctcatagtat ttctaaga
//
LOCUS       DAZGBC010000706          408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZGBC010000706 DAZGBC010000000
VERSION     DAZGBC010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.710"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..246
                     /locus_tag="ACHK1J_005745"
     CDS             <1..246
                     /locus_tag="ACHK1J_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914831.1"
                     /translation="IVTIEDVYTIAFSQPIHTESALGQRWMLIHTGEYESASDAKRFL
                     DTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT          127 a           97 c           84 g          100 t
ORIGIN      
        1 atcgttacaa ttgaagacgt ttacacaatc gcattctcgc aaccaattca caccgaatca
       61 gcgttggggc aaaggtggat gttgatccat actggtgaat atgaaagtgc cagcgatgca
      121 aaacgcttcc ttgatacgct cgtcaccggg cactgtgctt gcatggtaac accatcacct
      181 gttctggatc atgacacctg gctgcgtatc cgcctgctga tgggcgagat aagggatgcc
      241 aaataaacat tcatggcccc aacacataaa aataacgccg gggactctgg cagaggtacc
      301 attcttttta catagagcca atatataaac aacttttcta tgataatatc tttatcatgt
      361 tataaataat tgccccctca gcagaaaggg cccaaataga aaaagcaa
//
LOCUS       DAZGBC010000707          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZGBC010000707 DAZGBC010000000
VERSION     DAZGBC010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.711"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..349)
                     /locus_tag="ACHK1J_005746"
     CDS             complement(<1..349)
                     /locus_tag="ACHK1J_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914832.1"
                     /translation="MFPVSSIGNDISSDLIRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCVITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSD
                     NTCELTINEPLKNQ"
BASE COUNT          127 a           64 c           70 g          142 t
ORIGIN      
        1 cctggttttt taacggttca ttaattgtaa gttcgcaggt attgtctgat cgttgaataa
       61 ttcgggcgtt ataaccaaaa aaatccatac gaagagacga atcattataa agaattacat
      121 tttgtaagta cgctaattct ttttgaaaat caccagtgat aacacatttc ccacctggtt
      181 gtaacgcgtt aagtaaagtg accatttcaa tagaggtctg ttttagtttt tctatacctg
      241 gtgctagtgc ttcgaggtta tttcctgttg ggctttcagg aaggtcattc atttttcgac
      301 ggattaaatc actactaata tcattaccaa ttgatgaaac agggaacatt attttatcct
      361 ttataaataa tcaataaaaa tcattattaa aatgatcggg tga
//
LOCUS       DAZGBC010000708          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZGBC010000708 DAZGBC010000000
VERSION     DAZGBC010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.712"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>402
                     /locus_tag="ACHK1J_005747"
     CDS             <1..>402
                     /locus_tag="ACHK1J_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7914833.1"
                     /translation="ELIRELNSVLRGFPLFWNGQELQRSVVIDAINRKQPEIIKALVA
                     NEKIKSVYGTEIDGVLIFEFERLCSLLKYKEYWANSYTRYRNKIGLTSEGKYLDYNSD
                     VVLDFPFKDCVLEGGMTKEEQGKDEIYYNEI"
BASE COUNT          134 a           60 c           89 g          119 t
ORIGIN      
        1 tgaattaatt cgggaactga attctgtttt acgtggattt cctttgttct ggaacgggca
       61 ggaattacag cgctctgttg tgatagatgc cattaaccgc aagcaaccgg aaattatcaa
      121 ggctctggtt gcaaatgaaa aaatcaaatc agtatatggt actgaaattg atggtgtttt
      181 aatttttgaa tttgaaaggc tgtgcagcct cctgaaatat aaagaatact gggcaaacag
      241 ttacaccaga tatcgtaata agattggttt aacaagcgaa ggtaaatacc ttgattataa
      301 ttcagacgtt gtgctggact ttccctttaa ggattgtgtc cttgaaggcg gcatgacaaa
      361 agaagaacag ggtaaagatg aaatctatta caatgaaata at
//
LOCUS       DAZGBC010000709          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZGBC010000709 DAZGBC010000000
VERSION     DAZGBC010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.713"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..>399
                     /locus_tag="ACHK1J_005748"
     CDS             94..>399
                     /locus_tag="ACHK1J_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7914834.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFFWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSLESV
                     "
BASE COUNT          119 a           67 c           80 g          133 t
ORIGIN      
        1 gtattcctgt ggatgaagaa gcaaagtttt ttaatccatt tccaccaaga gcgtttgaaa
       61 ataaacctct ttatactgag gagataacta aatatgatta agggaattat gtttgcattt
      121 cttgctgctt tttcatgggg agcagcaata gtgatgagca aaaaaggtct cgaaaacatg
      181 gatgccggag agttgttttt ctggcaagtt ggtagtgctg caatgttatc atggtttgtt
      241 ctcgcaatta gcagaaaaaa actcccggta acaaaaaaat caatccttgc ctattcaaca
      301 ggaatttttg agccatttct ggcgtataca ttcactctct atggcctaaa atttatttct
      361 gctggtataa cagctgttat tttttcactt gagtcagta
//
LOCUS       DAZGBC010000710          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZGBC010000710 DAZGBC010000000
VERSION     DAZGBC010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.714"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..224)
                     /locus_tag="ACHK1J_005749"
     CDS             complement(<1..224)
                     /locus_tag="ACHK1J_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914835.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMP"
     gene            complement(205..>397)
                     /locus_tag="ACHK1J_005750"
     CDS             complement(205..>397)
                     /locus_tag="ACHK1J_005750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO7914836.1"
                     /translation="TAARAAIDVNNQAFRQFGFGGTPWVLSDTGWHLPSGILKDATTV
                     SLFLKATDTEDNHNEPASR"
BASE COUNT           92 a           96 c          131 g           78 t
ORIGIN      
        1 atgggcatac gcagggggca tcgccagcgt tgcagcgtga atatcacgga ggtgaccagg
       61 gtgaacagca gacagacagg aagggccagt ggccatatca gggccgtaat gatactgccg
      121 gccagacaca gaccaatgat gttcaggttg tgcagtgcat tccacagcgg gtttccccag
      181 gccgtgcggt gtatgaggtc aggattaacg tgatgctggc tcattgtgat tatcctcggt
      241 atctgtggct ttcaggaaca ggctgacggt agtggcgtct ttcaggatgc cggacggcag
      301 gtgccagccg gtatcggata acacccatgg ggtaccgcca aaaccgaact ggcggaaagc
      361 ctggttattg acatcgatgg cagcacgagc agcggtc
//
LOCUS       DAZGBC010000711          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZGBC010000711 DAZGBC010000000
VERSION     DAZGBC010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.715"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHK1J_005751"
     CDS             complement(<1..260)
                     /locus_tag="ACHK1J_005751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO7914837.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGKSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTI"
BASE COUNT          141 a           78 c           58 g          120 t
ORIGIN      
        1 ctgattgtta caaccagatc agggtcggta gtaattatta tctcaaaact acctgtaagc
       61 tcgccataag gaacgactat tgtattttct ggtaactcag aaaaagcatc actttttcct
      121 aacgaaacat cattaccaat gataacaagt agacgtccat taagtatttc atactcgcat
      181 ccattcaatg gtccgctcaa caaacgaatg gcataagatt ccaataagtc ttgtgataag
      241 aatttatcat tattttccat gaataccacc cgctaattcc ataagctcta gtttagagta
      301 gtgtaacaag acggaaattt catgaccaaa ttatgatgac acagaataaa aaaattataa
      361 tttcgaggac tattttaaat gcctctatgc ataactt
//
LOCUS       DAZGBC010000712          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZGBC010000712 DAZGBC010000000
VERSION     DAZGBC010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.716"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>397
                     /locus_tag="ACHK1J_005752"
     CDS             <1..>397
                     /locus_tag="ACHK1J_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7914838.1"
                     /translation="GGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAP
                     APSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCR
                     LLSPLEEALHGYVMTDGKLHADDTPVQVLL"
BASE COUNT           72 a          116 c          130 g           79 t
ORIGIN      
        1 ggcggttcac tgagctatct gggcgaggat accgccgaac agctggagtt gatgcgtagc
       61 gccttccggg ttatccggac ggtacgggaa aaacatgcct gtactcagtg cgatgccatc
      121 gtgcaggcac ctgcaccttc gcggcccatc gagcggggta tcgccggacc ggggctgctg
      181 gcccgcgtgc tgacctcgaa gtatgcagag cacaccccgc tgtatcgcca gtcagaaata
      241 tacggccggc aaggtgtgga gctgagccgt tcactgctgt cgggctgggt ggatgcatgc
      301 tgccggctgc tgtctccgct ggaagaggcg cttcatggct atgtcatgac tgacggcaaa
      361 ctccatgccg atgatacccc ggtccaggta ctgctgc
//
LOCUS       DAZGBC010000713          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZGBC010000713 DAZGBC010000000
VERSION     DAZGBC010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.717"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..124
                     /locus_tag="ACHK1J_005753"
     tRNA            48..124
                     /locus_tag="ACHK1J_005753"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
     gene            167..242
                     /locus_tag="ACHK1J_005754"
     tRNA            167..242
                     /locus_tag="ACHK1J_005754"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Ala,seq:tgc)
BASE COUNT          112 a           83 c           96 g          104 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg cacggcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc atagctccac
      241 catatctgta gtgattaaat aaaaaatact tcagagtgta cctgcaaagg ttcactgcga
      301 agttttgctc tttaaaaatc tggatcaagc tgaaaattga aacactgaac aacgagagtt
      361 gttcgtgagt ctctcaaatt ttcgcaactc tgaag
//
LOCUS       DAZGBC010000714          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZGBC010000714 DAZGBC010000000
VERSION     DAZGBC010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.718"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>394)
                     /locus_tag="ACHK1J_005755"
     CDS             complement(<1..>394)
                     /locus_tag="ACHK1J_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7914839.1"
                     /translation="KGALPPEQLNRCDMNTLQLEPTTFVDPDLRQFACDVLWSMKTTD
                     GRDGYIYTLTEHQSKADKYMALRMMRYVLAVIHRHLKAHACAPVVIPVLFYHGVPSPY
                     PYSMNWPDCLDDPAFGRELYGEGRPPRVI"
BASE COUNT           94 a          114 c           96 g           90 t
ORIGIN      
        1 caatcaccct gggtggcctg ccttctccat aaagctcccg accaaacgcc ggatcatcca
       61 gacagtcagg ccagttcatg ctgtatggat aaggactggg tacaccatgg taaaaaagca
      121 ccggtatcac aacgggagca cacgcatgtg ctttcaggtg gcgatgtatc accgccagaa
      181 catagcgcat cattcgcagg gccatatact tatccgcctt gctctggtgc tctgtcagcg
      241 tataaatgta tccatccctg ccatctgtgg ttttcatact ccacagaaca tcacaggcaa
      301 actggcgaag atccggatcc acaaacgtgg tcggctccag ttgcagggtg ttcatatcgc
      361 accggttcag ttgttccggg ggcaacgcgc cttt
//
LOCUS       DAZGBC010000715          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZGBC010000715 DAZGBC010000000
VERSION     DAZGBC010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.719"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>394)
                     /locus_tag="ACHK1J_005756"
     CDS             complement(<1..>394)
                     /locus_tag="ACHK1J_005756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO7914840.1"
                     /translation="MAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFNYM
                     VKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRLEGIEKGRLEGIEKGR
                     QEEREKALQENYASARRMQQMGISSEKIK"
BASE COUNT           89 a          117 c           74 g          114 t
ORIGIN      
        1 tttgattttc tctgacgata tacccatctg ctgcatgcga cgggccgatg cgtaattctc
       61 ctgaagagcc ttttctctct cctcctggcg gcccttctcg ataccttcca ggcggccctt
      121 ctcaatacct tccaggcggc ccttctcgat accagccagt tccagtcgtt cagcgatagt
      181 cattagatca tcctcatgtc ttggcagacg ttctgccatc gcccgtataa attcaggtga
      241 agctgaatca cattctttca ccatatagtt aaacaatgtc accacctgag tgtgtgtttc
      301 ctgtatctga atcaactgtg acaaaaagtc aagatgaacc atcagatcca ctttcttctg
      361 ccggagcttc atcataagca tcagcgcagc catc
//
LOCUS       DAZGBC010000716          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZGBC010000716 DAZGBC010000000
VERSION     DAZGBC010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.720"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..164
                     /locus_tag="ACHK1J_005757"
     CDS             <1..164
                     /locus_tag="ACHK1J_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7914841.1"
                     /translation="QGMKIMSVEKGMAWIRWQGSAWAVREGDRLGNITITRIEPSSRS
                     VITSAGTLR"
     gene            161..>393
                     /locus_tag="ACHK1J_005758"
     CDS             161..>393
                     /locus_tag="ACHK1J_005758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO7914842.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGG"
BASE COUNT           85 a           99 c          124 g           85 t
ORIGIN      
        1 tgcagggcat gaaaatcatg tcggtggaaa aaggaatggc ctggatccgc tggcagggta
       61 gcgcctgggc agtacgggaa ggtgacaggc tggggaatat caccatcacc cggattgaac
      121 cgtccagccg ctcagtcatc acctctgccg gtacattacg atgatgagca ttgatgccct
      181 gacagccata gagaactttg ccagcggtat attcagtgca ggcatggagt ttctcttcac
      241 ctggggagag ctgctgggga ttatcggcct gattggtcat ctgatgcgcg cccgggcaga
      301 agggcgtcac agtatgggac cgggaaaatt tatcgccggt atcgtcatct gcggcatgct
      361 ggtcgccctg ccatccttca tcaatgccgg ggg
//
LOCUS       DAZGBC010000717          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZGBC010000717 DAZGBC010000000
VERSION     DAZGBC010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.721"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..254
                     /locus_tag="ACHK1J_005759"
     CDS             <1..254
                     /locus_tag="ACHK1J_005759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7914843.1"
                     /translation="QLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSV
                     QRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
     gene            238..>389
                     /locus_tag="ACHK1J_005760"
     CDS             238..>389
                     /locus_tag="ACHK1J_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO7914844.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDA"
BASE COUNT           99 a           83 c          130 g           77 t
ORIGIN      
        1 agcaactggt ggcggaagag ctggtggaga agtgggaaaa aggaaagatg cgtctgctgt
       61 gggataacaa aaagcggcgt aacgaagcgc tggactgcct ggtgtatgcc tacgcggcat
      121 tacgtgtgtc cgtgcaacgc tggcagcttg atctggctgt actggcaaaa tcccgggaag
      181 aagagacgac ccggccaacc ctgaaagaac tggcagcgaa gctgtccgga ggagtgaatg
      241 gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc ttgatttatt aaccggtaaa
      301 cgggtggtgt ctgtccagaa agatggtcgc agaattgaat atacggcggc ttctctggat
      361 gagcttaacc gtgcgatcaa tgatgcgga
//
LOCUS       DAZGBC010000718          388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZGBC010000718 DAZGBC010000000
VERSION     DAZGBC010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.722"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>388
                     /gene="traF"
                     /locus_tag="ACHK1J_005761"
     CDS             <1..>388
                     /gene="traF"
                     /locus_tag="ACHK1J_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO7914845.1"
                     /translation="QSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAAL
                     MLKSYGTVSVDGKVSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALAT
                     ELETAGQKWSLGFTPKFQRVDLFNYNV"
BASE COUNT           92 a          104 c          106 g           86 t
ORIGIN      
        1 gtcagagcaa cgtgtcgcag actgctgcac gtctgaagac gcgacttcag gacttccgtc
       61 acacgcatgc tgacgcacag gtgggaacat ctgctgtggc gactcttccc ggtgacaccc
      121 tcgctgccgc tctgatgctg aaaagctacg gtacggtcag tgtcgacggt aaagtcagtg
      181 atgccgacct gaactatctg gaaagcattg caaacagtaa cggtcaggat gtggataaag
      241 acagactgac ctctcaggct tttgcccgtg ccgcactggt caccgatgtg ggtattgctc
      301 tggctactga actggaaacg gccggacaaa agtggtctct gggcttcaca ccaaaattcc
      361 agcgtgttga cctgtttaac tacaacgt
//
LOCUS       DAZGBC010000719          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZGBC010000719 DAZGBC010000000
VERSION     DAZGBC010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.723"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>383
                     /locus_tag="ACHK1J_005762"
     CDS             <1..>383
                     /locus_tag="ACHK1J_005762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914846.1"
                     /translation="DDWNDFSFITKYHLYITTKLTGLDEIEYAGVVKIIKKDQSEADY
                     YMVEEGEFSYLSDEFCSVGQSLDYYGRLSELGSEICEGILTSLRDINMFDYVSEGFKN
                     ERAWERSIMRDLNNDDDIFILPKFI"
BASE COUNT          120 a           40 c           86 g          137 t
ORIGIN      
        1 agatgattgg aatgatttta gttttatcac taagtatcat ttatacatta caacgaaact
       61 tactggtctt gatgaaatag agtatgctgg ggttgttaaa attataaaaa aagatcagtc
      121 tgaagctgat tactacatgg ttgaagaagg cgagttttca tatttgagtg acgagttttg
      181 ttctgtggga caatctcttg attattatgg gaggctctct gaattgggga gtgaaatttg
      241 tgaggggata ttgacatcat tacgcgacat taatatgttt gattatgttt ctgagggttt
      301 caaaaatgaa agagcttggg aacgttctat catgagagac ttaaataatg atgatgatat
      361 ttttattctt ccaaagttta taa
//
LOCUS       DAZGBC010000720          379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZGBC010000720 DAZGBC010000000
VERSION     DAZGBC010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.724"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHK1J_005763"
     CDS             <1..285
                     /locus_tag="ACHK1J_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7914847.1"
                     /translation="AATCRAGAAAEAGSKAAWERARTASQAWSQRENDVASSLQSCLS
                     RIKDVSITLPQFPSLDKLLRDLENQVCDAVVDRVNEHLPGNIDPWKDYNL"
BASE COUNT           92 a          126 c          101 g           60 t
ORIGIN      
        1 gcagcaacct gtcgggcagg tgctgccgca gaagccggca gcaaagccgc ctgggagcgg
       61 gcgcgcacag ccagccaggc atggtcacag cgagaaaacg acgtcgcctc ctccctgcag
      121 tcctgcctgt cccgcattaa ggacgtcagc atcactcttc cgcagttccc cagcctggac
      181 aaactcctcc gcgacctgga aaaccaggtc tgtgatgccg tcgtggatcg ggtgaatgaa
      241 catctgccgg ggaatatcga cccctggaaa gactataacc tctgaacagg agccccatga
      301 gcgaagcaca acccacctcc cctgtctcac cggaagcgga taacagcgga gatatttata
      361 accccgtgct cagaaccat
//
LOCUS       DAZGBC010000721          379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZGBC010000721 DAZGBC010000000
VERSION     DAZGBC010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.725"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHK1J_005764"
     CDS             <1..285
                     /locus_tag="ACHK1J_005764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7914848.1"
                     /translation="AATCRAGAAAEAGSKAAWERARTASQAWSQRENDVASSLQSCLS
                     RIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNEHLPGNIDPWKDYNL"
BASE COUNT           99 a          121 c           96 g           63 t
ORIGIN      
        1 gcagcaacct gtcgggcagg tgctgccgca gaagccggca gcaaagccgc ctgggagcgg
       61 gcgcgtacag ccagccaggc atggtcacag cgagaaaacg acgtcgcctc ctccctgcag
      121 tcctgcctgt cccgcattaa ggacgtcagc atcactcttc cacaattccc cagcctggac
      181 aaaatccttc gcgatctgga aaaccaggtc tgtgatgccg tcgtggatcg ggtgaatgaa
      241 cacctgccgg ggaatatcga cccctggaaa gactataacc tctgaacagg agccccatga
      301 gcgaagcaca ccccacatcc cctgtctctc cggaaacaga taacagcgga gatatttata
      361 aacaaacgct cagtaccat
//
LOCUS       DAZGBC010000722          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZGBC010000722 DAZGBC010000000
VERSION     DAZGBC010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.726"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>378
                     /locus_tag="ACHK1J_005765"
     CDS             <1..>378
                     /locus_tag="ACHK1J_005765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7914849.1"
                     /translation="EGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYG
                     LNVVSGDITDITTPDGRETKFYYNDGNLVTAVVYPDGLESRRAYDERDRLVTETSRSG
                     ETVRYRYDDAHSELPATTTDATG"
BASE COUNT           94 a           93 c          146 g           45 t
ORIGIN      
        1 cagaaggcgg ggccgggctg aagcgggtgg tgaaaaaaga actggcggac ggcagcgtca
       61 cgcacagcgg gtatgacgcg gcaggaaggc tcacggcgca gacggacgcg gcgggacggc
      121 ggacagagta cggtctgaat gtggtgtcag gcgacatcac ggacatcacc acaccggacg
      181 ggcgggagac gaaattttac tataacgacg ggaacctggt gacggcggtg gtgtacccgg
      241 acgggctgga gagccgccgg gcgtatgatg agcgggacag gctggtaacg gagacatcgc
      301 gcagcgggga gacagtacgc taccgctatg atgatgcgca cagtgaatta ccggccacaa
      361 caacagatgc gacgggca
//
LOCUS       DAZGBC010000723          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZGBC010000723 DAZGBC010000000
VERSION     DAZGBC010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.727"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..347)
                     /gene="pilM"
                     /locus_tag="ACHK1J_005766"
     CDS             complement(<1..347)
                     /gene="pilM"
                     /locus_tag="ACHK1J_005766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO7914850.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRV"
BASE COUNT           76 a          100 c          110 g           92 t
ORIGIN      
        1 tccaccctgc cgagcagagc agagtcatta ctctgttccc tgagcgcggc catcaggccc
       61 ggggtttctg tagcccagac aaaaacacgt tgctggctga tgatatgact gacggtatcc
      121 gtcccgggaa tcccgagctg agcggatgtc agccgccctc cggctgtccg tcgttccgga
      181 tgctgatgca ggtaatcatt gatggcgttc atataacgaa tgaacgttgc tgcctgttgt
      241 gtgttttctg cggcctgatg acggtgttcg ctgcgggaga gctgactggt gaaccagaaa
      301 ttgcctgtgg tcgtgatgac catgatgaac acgatgaacc agcccataag gttattcctg
      361 tggacgatct gtcacccg
//
LOCUS       DAZGBC010000724          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZGBC010000724 DAZGBC010000000
VERSION     DAZGBC010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.728"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>375
                     /locus_tag="ACHK1J_005767"
     CDS             <1..>375
                     /locus_tag="ACHK1J_005767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO7914851.1"
                     /translation="YGKPSLIMVDYLQLIRAPELENRTQEIAEISRSLKALGKELGCP
                     VLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMFIYRDEVYNPDSLAKGEAEI
                     IVSKQRQGPTGTVRVRFDGRYT"
BASE COUNT           84 a           99 c          115 g           77 t
ORIGIN      
        1 tgtacggtaa accctccctg attatggtgg attatctgca actgattcgt gcgccggagc
       61 tggaaaaccg cacacaggaa attgctgaaa tttcccgttc cctgaaagcg ctggggaagg
      121 agctgggctg cccggtgctg gccctctccc agcttaaccg gcaggtggag cagcgtgctg
      181 ataaacgccc caataacggt gacctgcgtg attccggggc gctggagcag gatgctgacc
      241 tgattatgtt tatttaccgc gacgaggtct ataaccccga ctcactggca aagggggagg
      301 cagaaatcat tgtcagcaag cagcgtcagg ggcccacggg tacggtacgg gtcaggttcg
      361 acggtcgtta cacac
//
LOCUS       DAZGBC010000725          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZGBC010000725 DAZGBC010000000
VERSION     DAZGBC010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.729"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..>364)
                     /locus_tag="ACHK1J_005768"
     CDS             complement(81..>364)
                     /locus_tag="ACHK1J_005768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFO7914852.1"
                     /translation="DGWKDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRH
                     QAVATVNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQPRLNYLTRQ"
BASE COUNT          101 a           84 c           55 g          124 t
ORIGIN      
        1 tttaacaact acggtacttc tgtatagttc attcaaacaa tcgtctatcg ttatgtctct
       61 ttctttcaaa gaatgtatat ttattgacgt gttaaataat tcaatctcgg ttgccacctc
      121 gtaataatat ccttgtaatc tttaaacatt ttcctgaaat actcacaact tacaggttgc
      181 ataaatatcc agacaacttt caatggctga ttgactgttg caaccgcctg atgtcgggat
      241 gcttgattaa ttgcgcttag attacctgtc caccagggtt tctttgaacc atcagccctg
      301 aaaaattggt cataccgcgc tttagcttcc cagaactgac acagcttatc tttccagcca
      361 tcaa
//
LOCUS       DAZGBC010000726          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZGBC010000726 DAZGBC010000000
VERSION     DAZGBC010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.730"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>362
                     /locus_tag="ACHK1J_005769"
     CDS             <1..>362
                     /locus_tag="ACHK1J_005769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL/TraM family protein"
                     /protein_id="HFO7914853.1"
                     /translation="ALVPGLVRALLCSSAALVISLTGNIIQYWHNSNVTREYFATDNG
                     RLVRLAPTSQPAWSQSDVMTFGSMTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVN
                     ALQASNILDTIKKEKFNLT"
BASE COUNT           86 a          108 c           88 g           80 t
ORIGIN      
        1 gcactggttc ccggactggt cagggccttg ctgtgtagca gcgcagctct cgtcatcagt
       61 ctgaccggca atatcataca gtactggcat aacagcaacg taacgcgtga atactttgcc
      121 accgacaatg gccggctggt ccgcctggcc cccaccagtc agccagcctg gagtcagagc
      181 gacgtcatga cgtttggcag catgacgctg agcgaagctt ttaatctgga ttttgtccac
      241 tacaggaaac aaatcacgac actggccccc cgcttttccg aagccggctt tgccggctat
      301 gtgaatgctc ttcaggcctc caacattctg gacaccatta aaaaggagaa atttaacctc
      361 ac
//
LOCUS       DAZGBC010000727          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZGBC010000727 DAZGBC010000000
VERSION     DAZGBC010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.731"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHK1J_005770"
     CDS             complement(<1..275)
                     /locus_tag="ACHK1J_005770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7914854.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA"
BASE COUNT           97 a          116 c           73 g           76 t
ORIGIN      
        1 gccatcccgg tgaccagccc gaaaagattg gacactgtca gcgacgggcg ggcactgctg
       61 ccctttccgt tcatctcaaa gccgctgccc tcaatcgggt acgcctgata ttcacgcccc
      121 tgccaggtca ccggctcccc tttttcattc agctcattgc agaaaaaata ccgctcaccg
      181 ccctgcaccg tcaggtcaat ttcccagagc accacccgtg gtgactgctc tgacttaacc
      241 gactcgttca ggctttcttc gtgaatatcc tgcatatatc cgcccataaa aaaggggcgc
      301 agacgcgccc cgaacaataa aagtccaatt taaaataaaa ccaataaagg taataacaga
      361 aa
//
LOCUS       DAZGBC010000728          361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZGBC010000728 DAZGBC010000000
VERSION     DAZGBC010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.732"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHK1J_005771"
     CDS             <1..315
                     /locus_tag="ACHK1J_005771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO7914855.1"
                     /translation="TGALTGDTGGRLALFVKAKECGTCDARLASVLATGRQVDIYLVD
                     SQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRFGEGKMPVVLQQGADGWRVA
                     AF"
BASE COUNT           68 a           84 c          129 g           80 t
ORIGIN      
        1 acaggagcac ttaccggaga tacgggggga cggctggcgc tgtttgtgaa ggcgaaggaa
       61 tgtggcacct gtgatgcccg tcttgcctcc gtactggcca cgggacggca ggttgatatc
      121 taccttgtcg acagtcaggg aaaggatgag attctgcgtc agtgggcgca tgcgcacagc
      181 atacctgtgg agaaagttcg cagccgtcat atcaccctga atcatgatgc cggacgctgg
      241 ctgcgttttg gtgaaggaaa aatgccggtg gtactgcagc agggggctga cggatggcgc
      301 gtggcggcat tctgacggtt ctgctgatgg tggcctgcag tggtgtctgt cgtgctgccg
      361 t
//
LOCUS       DAZGBC010000729          361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZGBC010000729 DAZGBC010000000
VERSION     DAZGBC010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.733"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHK1J_005772"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHK1J_005772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7914856.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
BASE COUNT           93 a          105 c           89 g           74 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 c
//
LOCUS       DAZGBC010000730          361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZGBC010000730 DAZGBC010000000
VERSION     DAZGBC010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.734"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHK1J_005773"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHK1J_005773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7914857.1"
                     /translation="MKIFRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     EQVEFNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVANVEQ"
BASE COUNT           92 a          108 c           93 g           68 t
ORIGIN      
        1 tcctgctcaa cgttggcaac gcctggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgcggt cagccagctg accggcatct aactcacgtg gcgccggagg cagatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 aacccggacg acagaagact gtcattaaac tcaacctgtt ccactcccca gggaaacggg
      241 gatgccagcg cagagatacc ggcactgcga aaagactccc attcagcctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggaaaatctt catttcccga
      361 c
//
LOCUS       DAZGBC010000731          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZGBC010000731 DAZGBC010000000
VERSION     DAZGBC010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.735"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..>356
                     /locus_tag="ACHK1J_005774"
     CDS             111..>356
                     /locus_tag="ACHK1J_005774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HFO7914858.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRS"
BASE COUNT           77 a           97 c          113 g           69 t
ORIGIN      
        1 tggcgggtgc tgaagaaaga attctcagtc ctgaacacag acctgatgcg cccgggttac
       61 ggcgaagggc ggtagcgtgc ctgacggtct cctgacagtg agtgaataac atggaaaatc
      121 cggcagccct gttacgtcgt cttaatcatt actgtgcccg tgcactggaa ggcgcagcct
      181 ccctttgcca gacccgggcc catgcagaaa tcaccccgga gcactggtta ctgaaactgc
      241 tggagcaggg ggaaggagac cttaccgtgc tgggccggcg ttatgactgg gatatggacg
      301 cgatatggca gtcgctgctc ggctggctgg ataaccagcc ccgtagcgta cgcagc
//
LOCUS       DAZGBC010000732          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZGBC010000732 DAZGBC010000000
VERSION     DAZGBC010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.736"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>352
                     /locus_tag="ACHK1J_005775"
     CDS             <1..>352
                     /locus_tag="ACHK1J_005775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFO7914859.1"
                     /translation="QPVDGIIQVMSISALPTDGERDALLRYRQKADHQLGWQAPVWLW
                     LTDNAIGARQDAKTAATGVIFGPEGTVKGADEAFSTLALHLQKAGMAQILNEPADFGL
                     LQLSLRLRQELKASL"
BASE COUNT           81 a           99 c          108 g           64 t
ORIGIN      
        1 caaccggttg atggcatcat tcaggtgatg agcatttcag ccctcccgac ggatggcgaa
       61 cgggatgccc tcctgcgtta ccgccagaaa gccgaccatc agctgggctg gcaggcaccg
      121 gtctggctgt ggctgactga caacgctatc ggtgcccggc aggacgcgaa aacggccgca
      181 acaggggtca tcttcggacc ggaaggaacc gtgaaaggtg cggacgaagc gttttccaca
      241 ctggcgttac acctgcaaaa agcaggaatg gcgcagatac tgaatgaacc tgctgatttc
      301 ggtctccttc agttgtcact cagactgcgg caggagctga aagccagcct gg
//
LOCUS       DAZGBC010000733          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZGBC010000733 DAZGBC010000000
VERSION     DAZGBC010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.737"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>352
                     /locus_tag="ACHK1J_005776"
     CDS             <1..>352
                     /locus_tag="ACHK1J_005776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFO7914860.1"
                     /translation="QPVDGIIQVMSISALLTDGERDALLRYRQKADHQLGWQAPVWLW
                     LTDNATGARQDAKAAATGVIFGPEGTVNGADEAFSTLALHLQKAGMAQILNEPADFGL
                     LQLSLRLRQELKASL"
BASE COUNT           79 a           97 c          108 g           68 t
ORIGIN      
        1 caaccggttg atggcatcat tcaggtgatg agtatttcag ccctcctgac ggatggcgaa
       61 cgggatgccc tcctgcgtta ccgtcagaaa gccgaccatc agttgggctg gcaggcacca
      121 gtctggctgt ggctgactga caacgctacc ggtgcccggc aggacgcgaa agcggccgca
      181 acaggggtca tcttcggacc ggaaggtacc gtgaacggtg cggacgaagc gttttccaca
      241 ctggcgttac acctgcaaaa agcaggaatg gcgcagatac tgaatgaacc tgctgatttc
      301 ggtctccttc agttgtcact cagactgcgg caggagctga aagccagcct gg
//
LOCUS       DAZGBC010000734          350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZGBC010000734 DAZGBC010000000
VERSION     DAZGBC010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.738"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..>350
                     /locus_tag="ACHK1J_005777"
     CDS             81..>350
                     /locus_tag="ACHK1J_005777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7914861.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGTAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYH"
BASE COUNT          140 a           48 c           59 g          103 t
ORIGIN      
        1 aaattaaaaa agcaatcagg gatgtaaaag tcaaactagg cttgaagagc aaataatgat
       61 tatgatgaag ggtaattaaa ttggatttta aacatcttac tcaatttaaa gatataattg
      121 aactggacaa gcgccccgtt aaacttgatg aacgggaaac gtttaatgtc tcatggggta
      181 ttgatgagaa ctaccaggtt gggactgcga tttcaattgc ttcaattctt gaaaataata
      241 aacaaaacaa atttaccttt cacataatcg ctgattactt agacaaagag tatattgaat
      301 tattatcaca attagcaacg aagtatcaaa cagtaattaa attatatcat
//
LOCUS       DAZGBC010000735          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZGBC010000735 DAZGBC010000000
VERSION     DAZGBC010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.739"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>349
                     /locus_tag="ACHK1J_005778"
     CDS             <1..>349
                     /locus_tag="ACHK1J_005778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO7914862.1"
                     /translation="PEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLK
                     RASEVVIATDADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQT
                     ALLSEAGKGRARAD"
BASE COUNT           82 a           73 c          115 g           79 t
ORIGIN      
        1 ccggaagctt acggtgaaca gttcggttcc ccatggcgtt ctgatgtgtt gccgctgata
       61 ccggaacaat ggcagatggt ggtgaagccg aaggtgaaaa aacaacttat tgtgatccgg
      121 gggctgttaa aacgcgcttc tgaagtggtt attgccacag atgccgatcg tgaaggagaa
      181 gtgatagcca gagagttgct ggagtactgt cagtacagag ggcgtgtcag gcgtctgtgg
      241 ttatctgcac tggatgaggc cagtatcagg aaggcgctga ataatattct cccgggcgaa
      301 cagactgcgc ttctcagtga agccggtaag gggcgtgccc gggctgact
//
LOCUS       DAZGBC010000736          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZGBC010000736 DAZGBC010000000
VERSION     DAZGBC010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.740"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>348
                     /locus_tag="ACHK1J_005779"
     CDS             <1..>348
                     /locus_tag="ACHK1J_005779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7914863.1"
                     /translation="GYHRVRKPNHYVRNITHSVIKNTYVLPEALTGQIREEEAIMLTS
                     QLQALPEELAQGVLESLSGMLGSHSLQNPVGWLLAVLKRAREGNFYPPKQKQRVVVPA
                     SVLAGERGRATAQS"
BASE COUNT           89 a           79 c          111 g           69 t
ORIGIN      
        1 aggttatcac agagttcgga aaccgaacca ttacgtacgt aatatcacac acagtgtgat
       61 taaaaacacg tacgtattgc ctgaagcgct gacagggcaa atcagggagg aggaggccat
      121 tatgctgacc agtcagttgc aggcgctgcc ggaagaactg gcgcaggggg ttctggaaag
      181 tctgtcaggg atgctgggca gccactcgct gcagaatcct gtgggctggt tgcttgcagt
      241 gctgaagcga gcccgggagg gaaattttta tccgccgaag cagaaacaac gtgttgttgt
      301 gccagcctcc gtactggcag gagaaagggg gcgagctact gcgcaatc
//
LOCUS       DAZGBC010000737          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZGBC010000737 DAZGBC010000000
VERSION     DAZGBC010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.741"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..224
                     /locus_tag="ACHK1J_005780"
     CDS             <1..224
                     /locus_tag="ACHK1J_005780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914864.1"
                     /translation="ASRRNVAGGKYPRTLVMNGCFYISCFLMNCVSVVNIHGGKFSGQ
                     KNPPVSSGSTASCFWWKWYGYEVLLLCMP"
BASE COUNT           91 a           64 c           83 g          110 t
ORIGIN      
        1 ccgccagtcg caggaatgtc gctggcggta aatacccacg aacccttgta atgaatggat
       61 gtttttacat cagttgtttt ttaatgaatt gcgtttctgt tgtcaacatt catggtggga
      121 agttttcagg acaaaaaaat ccaccagtaa gctcagggag tactgcgtca tgcttctggt
      181 ggaaatggta cggctatgag gttttattgt tatgcatgcc ataatttttc tacacaaaag
      241 caattctgaa tgtcaatacg ttttataagg aattgttgtg cacacagagt aaatacacgg
      301 ggctgtcagt gtaacactgg cggccttttt catgttgtga gcttccgg
//
LOCUS       DAZGBC010000738          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZGBC010000738 DAZGBC010000000
VERSION     DAZGBC010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.742"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>347
                     /locus_tag="ACHK1J_005781"
     CDS             <1..>347
                     /locus_tag="ACHK1J_005781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914865.1"
                     /translation="TPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSKKRKYEKKL
                     KEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVPRVRELITW
                     YEKYAPFTELPED"
BASE COUNT          117 a           78 c           61 g           91 t
ORIGIN      
        1 actcctggca tgaacgacat catcagtata atcattctcc tgacagtcta tctcatcggc
       61 ctgatattca ttatcctgac tatcaatgat atagacagat catcaaaaaa gagaaagtac
      121 gagaaaaaac tgaaagaagc gataataaat ggcagtttac aaaataatga tatctgttta
      181 ctggccgaac gctggtctgc cagtcatgaa catatcaaaa gcgcactgct gtctgttttc
      241 aatgattttc tgactgataa caacttttca catcacgtcc cccgggtcag agaactgatc
      301 acatggtacg agaaatatgc cccattcaca gaactccctg aggatat
//
LOCUS       DAZGBC010000739          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZGBC010000739 DAZGBC010000000
VERSION     DAZGBC010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.743"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          108 a           59 c           81 g           99 t
ORIGIN      
        1 cgtgtgaatg atgatgaaga taatttattc ttagaatgga ttgtaaaaga tggttattta
       61 gaggaaataa ttttgtctgg ttcataaatt catcttcttt gttgagaaga ttaactgcct
      121 aggtgaaatt atcctctatg accgggacgc tgcaggccac ctggcagaag tcatcacgca
      181 ggaagacaag tcgctgtatc cctttgaaaa atccggcagg ctaatgggca tcttcagccc
      241 aggcggcaca tcacagcgaa tttgaaggca aatgcgaaaa ttagtgatct tgaagttcat
      301 acagtattgg taaaaacagg cgaagctttt ccaatgtgta agaattt
//
LOCUS       DAZGBC010000740          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZGBC010000740 DAZGBC010000000
VERSION     DAZGBC010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.744"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>347
                     /locus_tag="ACHK1J_005782"
     CDS             <1..>347
                     /locus_tag="ACHK1J_005782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914866.1"
                     /translation="EKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYGVL
                     MLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVLTA
                     SWKEQLENLPEML"
BASE COUNT           88 a           79 c          115 g           65 t
ORIGIN      
        1 gtgagaaaca gccgcagata ccggagggtg cgtctggtat gtcgccggag cgaagaaaaa
       61 gtcgtttccc cggcgtgttg atactgtctg tcactgtggg gctggtcatg ggggcagtgg
      121 tatatggcgt actgatgctt atgaccggtc ttgacagccg gatcacgacc ctggaaggcc
      181 agagaagaga aatgctttct gcgtcagaac tcgcgcccct gagaatgaaa ctgacagaga
      241 cggagaagca actgaaggat atggaaaagc gcctggcagt cctggagcag cagcccgtgc
      301 tgacagcatc ctggaaggaa caactggaga atctgccgga gatgctg
//
LOCUS       DAZGBC010000741          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZGBC010000741 DAZGBC010000000
VERSION     DAZGBC010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.745"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..223)
                     /locus_tag="ACHK1J_005783"
     CDS             complement(<1..223)
                     /locus_tag="ACHK1J_005783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914867.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQE"
BASE COUNT           63 a          116 c           76 g           90 t
ORIGIN      
        1 gctcctgctc cccggcacgc cagacggcgg tcacaccgtg tatcacggga ttaccgtccg
       61 tgtccgtcag cggggttttg ttcaccagaa tactctgcag tcccttcacc ggaccttcca
      121 ccggtccctc accaatggca tcaatcacgc tcatcatctg cgtggatttg agattgtcct
      181 tcgcctcacg cggtgtgtgc cccttgccgc cccctttacc cactctgtcc ccctctcctg
      241 tctgatgtct gaatctgttt atgccagaaa acaacaggca ccccggaggg tgcctgtgtc
      301 atgacggaat aaaatttctg aaattcttca catttccggc aattg
//
LOCUS       DAZGBC010000742          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZGBC010000742 DAZGBC010000000
VERSION     DAZGBC010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.746"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>345
                     /locus_tag="ACHK1J_005784"
     CDS             <1..>345
                     /locus_tag="ACHK1J_005784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO7914868.1"
                     /translation="SDATGTQHTATTQPEEPQQVSMGSADDWSAFAEDLNADAPGNAT
                     VHSTDNAETKTLSEPAGMQPVQQATPEPTEENKPQEEGVMSATDTGEESAAAAIDDDL
                     NTVAEDLNADVT"
BASE COUNT           98 a          100 c           91 g           56 t
ORIGIN      
        1 tctccgacgc aaccgggaca cagcacaccg ccacaacgca acctgaagaa ccacagcagg
       61 tctccatggg cagcgctgac gactggagcg catttgcgga agacctcaat gctgatgctc
      121 cggggaatgc cactgttcac agcaccgaca acgcggagac aaagacactt tctgaacctg
      181 ccgggatgca gcctgttcaa caggcaaccc ctgaaccaac ggaagaaaat aaacctcagg
      241 aagagggcgt tatgtctgct actgatacag gtgaagagag tgccgctgcc gctatcgatg
      301 acgacctgaa cacagttgcg gaagacctca atgctgatgt tactg
//
LOCUS       DAZGBC010000743          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZGBC010000743 DAZGBC010000000
VERSION     DAZGBC010000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.747"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..319)
                     /locus_tag="ACHK1J_005785"
     CDS             complement(<1..319)
                     /locus_tag="ACHK1J_005785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914869.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSG"
BASE COUNT           94 a           75 c           49 g          122 t
ORIGIN      
        1 ttccactact agaagaggat gttgatgagt taaatcctga gtttaataaa cctccattgt
       61 agtttggatt aaaacttcca agtcctatac ccggattata gttaataccg tttgatggta
      121 ttgttattgt cctatttgaa ccatcagcaa taactcctcc aatatatcct ccaattgcac
      181 ttcctgctat atccgcacca gggatttttg ttgctttacc taaaaaacca ccagccgcag
      241 tacctattgc aacggatgca tctttatcta atcctgcacc atgtattaaa gcaagttcat
      301 cggttgttat atttctcatg ttatttcctc tttttattaa
//
LOCUS       DAZGBC010000744          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZGBC010000744 DAZGBC010000000
VERSION     DAZGBC010000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.748"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>340
                     /locus_tag="ACHK1J_005786"
     CDS             <1..>340
                     /locus_tag="ACHK1J_005786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFO7914870.1"
                     /translation="LIKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLV
                     AHGANNFYESLSPLFFNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGG
                     YSGMKIVPKYN"
BASE COUNT          106 a           47 c           79 g          108 t
ORIGIN      
        1 ttgataaaat atgtgaactt aagtttcgat attgttgcat ctgtaggaca ggtcgtaggc
       61 ggggttggcg cgttgaaatt tggtaaggta gtgcattcaa accgaataaa aggtataggc
      121 gtcacattag ttgctcatgg ggcgaataat ttttatgaaa gcttatcacc tttatttttt
      181 aacgagtatg attcaggtcc aatacgtgaa ctctacagag taatagcaaa aaaaatggga
      241 ggtgatatta attctgctga ttatgcatac agcatcgtgg acttttcgat tactgcatat
      301 ggtggatatt caggcatgaa aatcgtacct aagtataata
//
LOCUS       DAZGBC010000745          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZGBC010000745 DAZGBC010000000
VERSION     DAZGBC010000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.749"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>338)
                     /locus_tag="ACHK1J_005787"
     CDS             complement(<1..>338)
                     /locus_tag="ACHK1J_005787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO7914871.1"
                     /translation="SLIYYINNWNLFPLICLLSGCHFYRERFAERGFFYKVPAVLRDY
                     LSAIPVEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHI
                     QLPLPLASTL"
BASE COUNT          117 a           56 c           67 g           98 t
ORIGIN      
        1 aaagagttga cgcaagtggt aacggcaatt gtatgtggtc aacaaaatca ggaaataata
       61 aattaaatcg ttgctgcatt gccaatggtt gttgccttat atagggtaac aacgttgaaa
      121 aaccacatgt gatgatattg tgatagttag cgatgccggg tttgtatcgt gccttttcat
      181 ttatttccac cggtatagct gataaataat cccttagcac agcaggaacc ttataaaaaa
      241 atccgcgttc tgcaaacctt tctcgataaa agtgacaacc actgagtaaa cagatcaatg
      301 gaaataaatt ccaattgttt atgtagtaaa taagtgaa
//
LOCUS       DAZGBC010000746          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZGBC010000746 DAZGBC010000000
VERSION     DAZGBC010000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.750"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..>338
                     /locus_tag="ACHK1J_005788"
     CDS             43..>338
                     /locus_tag="ACHK1J_005788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO7914872.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDT"
BASE COUNT           85 a           98 c           94 g           61 t
ORIGIN      
        1 aatggtcagt ccattcacgt cacccctgaa ctggagtaaa tcatgagcgc agaacaggac
       61 acagccgcaa agtccggcaa aaaactgtca gccctgctgg ttctcggggg cgtgattatc
      121 atcgggggcg gatacctgct gctcacctgg ctttcccccg aaaacagcgc atcgctctcg
      181 tcagtcaata tcaacagcac gacaacaggc agcgggcgga ccgtggtgga gtcacagcac
      241 taccgggatt tactgcgtgc tgacaatgag cgcggggccg cagaagcatt acgtaataac
      301 cagacgttca tcgccagtct gcccagggga ctggatac
//
LOCUS       DAZGBC010000747          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZGBC010000747 DAZGBC010000000
VERSION     DAZGBC010000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.751"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..135
                     /gene="pilP"
                     /locus_tag="ACHK1J_005789"
     CDS             <1..135
                     /gene="pilP"
                     /locus_tag="ACHK1J_005789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO7914873.1"
                     /translation="ELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP
                     "
BASE COUNT           92 a           88 c           98 g           60 t
ORIGIN      
        1 gaacttccgg ggggaagcat ctccgatgtg aaggcgggcc agaacatccc cggaaccact
       61 tatcacgtgg aaaaaatcac gccggacagt gtggtgctgg aacagtctgg caccagaaaa
      121 accctgcgac catgagaccg gccgccgtaa ctggcggctg tcaaaaataa aaggatgaaa
      181 gacatatttt ccgggcaccg ctgcccgtaa ggaggcttta tgctggatcc agcagacctg
      241 ccgccggatg acattctgga ttatgtggcc atcgacaccg ataacgccgg acaactgcgg
      301 atcagggttg ttgagggaaa aaaacatctg cgtgccgt
//
LOCUS       DAZGBC010000748          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZGBC010000748 DAZGBC010000000
VERSION     DAZGBC010000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.752"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>337
                     /locus_tag="ACHK1J_005790"
     CDS             <1..>337
                     /locus_tag="ACHK1J_005790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO7914874.1"
                     /translation="ASRETVSRVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPV
                     TCRDAEILDPRYLDMVADACMSRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARI
                     GSAQTPGQMA"
BASE COUNT           83 a           77 c          113 g           64 t
ORIGIN      
        1 gcctccaggg agactgtcag ccgggtgctg ctgatgctgc ggatcacggg ctggctgagt
       61 ctgtgcaaac gggtgcgtga tgagaaaggg cgggtacggg gcaatattta tgcccagcac
      121 gatgaaccgg tgacgtgccg ggatgctgaa atactggatc cacgttatct ggatatggtg
      181 gcagatgcct gtatgagccg gaaccggacc ataagtcaga cggcccggga tattctgtgc
      241 gaaataaaaa atgatccggg aatgcgtcat tatcacagcc atatcagtca actggaagcc
      301 cggataggga gtgcccagac accgggacag atggcgg
//
LOCUS       DAZGBC010000749          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZGBC010000749 DAZGBC010000000
VERSION     DAZGBC010000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.753"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..175)
                     /locus_tag="ACHK1J_005791"
     CDS             complement(<1..175)
                     /locus_tag="ACHK1J_005791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7914875.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWT"
     gene            complement(172..>333)
                     /locus_tag="ACHK1J_005792"
     CDS             complement(172..>333)
                     /locus_tag="ACHK1J_005792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO7914876.1"
                     /translation="LSGQSHATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAAL
                     FIPAGEFPR"
BASE COUNT           84 a           82 c           87 g           80 t
ORIGIN      
        1 ctgtccagcc ggaatatccc agaagcataa aaaacagcag aatgccgctg cacacccaga
       61 atgtcgtttt tgacggatac ggaaacaaca gcaggtaaat catggtaaac ggcaggggga
      121 caccataaat gttgagcggt ggtgttacat cccgccagaa gccttcgcgt ttcatcgggg
      181 gaactctccg gccgggatga agagcgctgc ttctgatttc tcaatgcggt cgttctgata
      241 catggtcaga acctgatccc ttatccgccg tttttcctga cgaatgattt cgtcaatgta
      301 atgtccccat gttgcatggg attgtccgga gag
//
LOCUS       DAZGBC010000750          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZGBC010000750 DAZGBC010000000
VERSION     DAZGBC010000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.754"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>329)
                     /locus_tag="ACHK1J_005793"
     CDS             complement(<1..>329)
                     /locus_tag="ACHK1J_005793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7914877.1"
                     /translation="GNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQFFGIP
                     PRQVWRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLIPRNT
                     YLVRTAY"
BASE COUNT           57 a           87 c          115 g           70 t
ORIGIN      
        1 tcgtatgcgg tgcggaccag gtacgtgttg cggggaatga gcacaccacc ctggcgggtg
       61 atataggtgg caaaacatcc cacgtcggcg gcggccagta tggcgtcaat gtcgtcatgg
      121 tccagacgct gcggggcagc cgggttacgg gccatttgcc cggcaagacg gcggagttcg
      181 cgccatacct ggcggggagg gataccgaag aactggaact ggtggatgcg gtgaaggcct
      241 gcccagccga tggcgttctc cacggtttcg gccatggttt tgcctgattc cttatcgacg
      301 taaggtttgc cggttttcgg gtcattccc
//
LOCUS       DAZGBC010000751          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZGBC010000751 DAZGBC010000000
VERSION     DAZGBC010000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.755"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>327)
                     /locus_tag="ACHK1J_005794"
     CDS             complement(<1..>327)
                     /locus_tag="ACHK1J_005794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO7914878.1"
                     /translation="TSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTEVDSDNAV
                     SRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALNEHTGMSS
                     VASDVNH"
BASE COUNT           61 a           92 c          103 g           71 t
ORIGIN      
        1 gtggttcacg tcgctggcca cggatgacat gccggtatgt tcattcaggg catatgcatt
       61 gactttcaga aacatgcccc gccacatcat ccggtcaaag tggatatcca ttcccttgcc
      121 ggcgagtctg gcgtcctggc tgtagaggac ggccccggcc catgggccgg caggtacgga
      181 ggccaccacg cgggagacag cattgtcaga gtccacttcc gtatcaatga aggcaggtac
      241 gcgggtcagt gccggtatca gctgtatccc tgtacgagct gccggggttg ccagactggc
      301 ctcctgcaga cgtggttgtg gtgatgt
//
LOCUS       DAZGBC010000752          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.756, whole genome shotgun sequence.
ACCESSION   DAZGBC010000752 DAZGBC010000000
VERSION     DAZGBC010000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.756"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..326
                     /locus_tag="ACHK1J_005795"
                     /pseudo
     CDS             1..326
                     /locus_tag="ACHK1J_005795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           87 a           72 c           95 g           72 t
ORIGIN      
        1 caaagaagcg gtggagtatg gtcagtcgcg aaaatggata gcgcatgcgc ccttgttgcc
       61 aacggacaag gaataaccct ggtaagccgt attctgggcg tgtcgcgtgc gcaactggca
      121 caacggatga aaacatctgg taataagccg aataaacgtc gtcgcagatg tgatgaggct
      181 gcggatgcgg aagtgttagc acgtatcctt gatatcatca gcgatatgcc gacatatggc
      241 tatcgtcgtg tctgggccat tctcagacga cagtcccgta acaaaggagt gccgttcgtg
      301 aatgcaaagc gcgtttatcg gataat
//
LOCUS       DAZGBC010000753          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.757, whole genome shotgun sequence.
ACCESSION   DAZGBC010000753 DAZGBC010000000
VERSION     DAZGBC010000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.757"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>326
                     /gene="trbA"
                     /locus_tag="ACHK1J_005796"
     CDS             <1..>326
                     /gene="trbA"
                     /locus_tag="ACHK1J_005796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO7914879.1"
                     /translation="SLARNAFIRVIKTEGAQKWLRQHRYVRSGLVWLYAHDLRLTPPN
                     WLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARAENEACRLGLPCPEPCVEEAIEG
                     LRQDML"
BASE COUNT           69 a           95 c          100 g           62 t
ORIGIN      
        1 tcactggcca gaaacgcctt tatccgggtc atcaaaacag aaggcgcaca aaagtggctc
       61 aggcagcacc gttacgttcg cagcggtctg gtctggcttt acgcacatga tttgcgcctg
      121 acgcctccga actggctgtg gctcaagggc gttgaccgga cgctcttcta tgcccttcac
      181 cgggcaaaca ccacaaaagg cttcatcgaa ggggccggcg tggtggccgt tgcccgggca
      241 gaaaatgaag cgtgtcgtct cggcctgccc tgcccggaac cctgtgtgga agaagccatt
      301 gaagggctgc ggcaggacat gctgag
//
LOCUS       DAZGBC010000754          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.758, whole genome shotgun sequence.
ACCESSION   DAZGBC010000754 DAZGBC010000000
VERSION     DAZGBC010000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.758"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          104 a           67 c           64 g           87 t
ORIGIN      
        1 tatgcagtac gcacaaaaaa aacaggctgg aattgcttgc tgatttataa tctttatcta
       61 aattactatt ggcgctttgg ataataacat aagcatatat tacctcctag caataataga
      121 gataacctca tatcaaggtg tcactggtaa ataatcggat aaaagacagt atcggtcggc
      181 gcatagagct tcttctattt caccagaagt gcacagcagg aactaccaat cacacggtta
      241 gtgtcatggg tggaggatgc ggcccgttct ttccctataa cagggttacg cagtttcaat
      301 cagtacatgc agaaaaaccc ct
//
LOCUS       DAZGBC010000755          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.759, whole genome shotgun sequence.
ACCESSION   DAZGBC010000755 DAZGBC010000000
VERSION     DAZGBC010000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.759"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>322
                     /locus_tag="ACHK1J_005797"
     CDS             <1..>322
                     /locus_tag="ACHK1J_005797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914880.1"
                     /translation="QKDPEFPDDCFDSMRADAHRCVKDQEQKKSGTKINSGTKITCCG
                     VELSKDILKILADKTKKYETAVEHTLGLVVADVCFKEDECSQVRELLKKPNGNVTDDK
                     IDEIV"
BASE COUNT          122 a           46 c           68 g           86 t
ORIGIN      
        1 tcagaaagat cctgaatttc ctgatgattg cttcgattct atgcgggcag atgctcatag
       61 atgtgtaaaa gatcaagaac aaaaaaaatc tggcacaaaa ataaatagcg gcaccaaaat
      121 tacttgttgc ggtgttgagt taagtaaaga tattcttaaa atattagcag ataaaactaa
      181 aaaatatgag accgctgttg aacataccct tggactcgtg gttgcagacg tatgctttaa
      241 agaagatgag tgttctcagg taagagagtt attaaaaaaa ccaaacggta acgttacgga
      301 tgataagatt gatgaaatag tc
//
LOCUS       DAZGBC010000756          321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.760, whole genome shotgun sequence.
ACCESSION   DAZGBC010000756 DAZGBC010000000
VERSION     DAZGBC010000756.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.760"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..>321
                     /locus_tag="ACHK1J_005798"
     CDS             29..>321
                     /locus_tag="ACHK1J_005798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7914881.1"
                     /translation="MNKETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKGFASVCQSKKKYQQAYDLY"
BASE COUNT          130 a           50 c           54 g           87 t
ORIGIN      
        1 atcacgagac acatcacaga ggaatattat gaacaaagaa acaattgaaa tattcaataa
       61 tagtgatgaa tgggcaaatc aactaaaaca cgcattatcg aaaggagaaa acctggcatt
      121 actgcatggt ttaactcctg atatccttga tagaatatat gcatatgcat tcgactacca
      181 tgaaaagggt aatataacgg acgcagaaat ttattataaa tttttgtgca tttatgcgtt
      241 cgaaaatcat gagtatctaa aaggttttgc atcagtatgt cagtcaaaaa agaaatatca
      301 acaagcatat gacctttaca a
//
LOCUS       DAZGBC010000757          321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.761, whole genome shotgun sequence.
ACCESSION   DAZGBC010000757 DAZGBC010000000
VERSION     DAZGBC010000757.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.761"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..139
                     /locus_tag="ACHK1J_005799"
     CDS             <1..139
                     /locus_tag="ACHK1J_005799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO7914882.1"
                     /translation="SGIRHIAINILTNDTVFKAGLRRKMRKAAMDRNHLASVLAGCGL
                     S"
BASE COUNT           97 a           52 c           68 g          104 t
ORIGIN      
        1 ttcagggata cggcacattg ctattaatat tttgacgaat gatacggtat tcaaggcagg
       61 gttaagacgt aagatgcgaa aagcagccat ggacagaaac cacctcgcgt cagtccttgc
      121 ggggtgcggg ctttcgtaat cttgccctgg tcttccaact atttcaggtc gaaattatgg
      181 agattaaaaa tgtcagctga atttatcctt tcatttcctg atgactcttg gtatttgaga
      241 aataggaata acattgttaa taaaatatta tttttgtcta catacagaaa aaatgatgga
      301 gatatgtatt atttatcttg t
//
LOCUS       DAZGBC010000758          313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.762, whole genome shotgun sequence.
ACCESSION   DAZGBC010000758 DAZGBC010000000
VERSION     DAZGBC010000758.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.762"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..>313
                     /locus_tag="ACHK1J_005800"
     CDS             118..>313
                     /locus_tag="ACHK1J_005800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7914883.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFL"
BASE COUNT          111 a           53 c           72 g           77 t
ORIGIN      
        1 tttaatgcag aaaacgaagc ccaacggatg ggcataaaaa tgagcgaatt ttgatatcac
       61 caccccctaa aaaatggtga agtctgagca aagaatgttc gaaaggagag agtagagatg
      121 gaaagcgcaa atctggattt agaagaaaat aaagaaattg ccagtaagtt cgaacgtgca
      181 ctaggtatgg gagcaacact tgcagagctg catggcatta ctccggatac ccttgaaggc
      241 gtttatgctt acgcttataa cttttatgaa aaaggaagac ttgacgaagc tgaactcttt
      301 tttaaatttc tct
//
LOCUS       DAZGBC010000759          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.763, whole genome shotgun sequence.
ACCESSION   DAZGBC010000759 DAZGBC010000000
VERSION     DAZGBC010000759.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.763"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>311
                     /locus_tag="ACHK1J_005801"
     CDS             <1..>311
                     /locus_tag="ACHK1J_005801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7914884.1"
                     /translation="LGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARL
                     PYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDPGDELQASHPL
                     R"
BASE COUNT           67 a           90 c           79 g           75 t
ORIGIN      
        1 ctgggcttta ttccgctctc ttactacaag aaccgggatt atgcctgctt cttctccgct
       61 aactccaccc agaaaccggc actgtacgat acgccggatg caacggcgaa cagccgtatt
      121 aatgcccgtc ttccctatat cttcctgctg tcccgcattg cgcattacct gaagatgatt
      181 cagcgggaaa atatcggtac caccaaagat cgccgtctgc ttgagctgga gctgaataac
      241 tggatccggg gtctggtgac ggagatgacc gatccgggtg atgagttgca ggcctcgcat
      301 ccgctgcgtg a
//
LOCUS       DAZGBC010000760          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.764, whole genome shotgun sequence.
ACCESSION   DAZGBC010000760 DAZGBC010000000
VERSION     DAZGBC010000760.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.764"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>311
                     /locus_tag="ACHK1J_005802"
     CDS             <1..>311
                     /locus_tag="ACHK1J_005802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914885.1"
                     /translation="KIDSALSWDAIDAIRDYSISDIYLYPEQKTINCVVEIENIVFGG
                     YIYTLNNGVTLQNIENTLKDSSCHYVLNGYTEFVNCLRQLTSGKTESVHHTINKLNYE
                     K"
BASE COUNT          113 a           51 c           51 g           96 t
ORIGIN      
        1 gaaaattgac agtgcattat cgtgggatgc cattgacgca atacgagact atagtatttc
       61 tgatatttat ctttatcctg aacaaaaaac aataaattgt gtcgttgaga ttgaaaacat
      121 tgtctttggc ggttacattt atacattgaa caacggcgtc acattacaaa acatagaaaa
      181 cactttaaaa gattcttcat gccattatgt cttaaatggc tatacagaat ttgttaactg
      241 tttgagacaa cttacttcag gaaagactga aagtgttcat cacaccatca ataaactgaa
      301 ctatgagaaa t
//
LOCUS       DAZGBC010000761          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.765, whole genome shotgun sequence.
ACCESSION   DAZGBC010000761 DAZGBC010000000
VERSION     DAZGBC010000761.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.765"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>311)
                     /locus_tag="ACHK1J_005803"
     CDS             complement(<1..>311)
                     /locus_tag="ACHK1J_005803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO7914886.1"
                     /translation="HLPDKDEMRGVPPADALRAVQLLWNEMSNPGGTFPVTHDTYLKL
                     FQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDCYQQIYRFRGANNAL
                     S"
BASE COUNT           75 a           98 c           78 g           60 t
ORIGIN      
        1 tgggaaagcg cattattcgc accccgaaag cggtaaatct gttgataaca atctccaaca
       61 aggaccaccc gacacttctg ccccagaacc agagcagtgg tcaccggatt cgcatcctgc
      121 gcctcatcaa acaggatggt gtcccatcgg tgagacaggt ctgcaccgga caactggaag
      181 agtttcagat aagtatcgtg cgtcaccggg aacgtccccc cggggttgct catttcgttc
      241 cagagcaact gcaccgcccg aagcgcatct gctggcggaa caccccgcat ctcatcctta
      301 tccggcaggt g
//
LOCUS       DAZGBC010000762          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.766, whole genome shotgun sequence.
ACCESSION   DAZGBC010000762 DAZGBC010000000
VERSION     DAZGBC010000762.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.766"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>308
                     /locus_tag="ACHK1J_005804"
     CDS             <1..>308
                     /locus_tag="ACHK1J_005804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7914887.1"
                     /translation="FLISYRPNWRWLGMRETAAKSFVDEVEAAWSEYAEGMSGEIDVE
                     GKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQLFRTRFKAVSPKRVDTPGHGMGNR
                     "
BASE COUNT           68 a           65 c          108 g           67 t
ORIGIN      
        1 tttctgatca gctaccgtcc gaactggcgc tggctgggga tgcgggagac tgcggcaaaa
       61 agttttgtcg atgaggtgga ggcggcctgg tcggaatacg ccgaagggat gtctggcgag
      121 atcgacgtgg aaggaaaacg cacgttcacg gaatttatcc gtgaaggtgt gggcgttcat
      181 gcgtttaacg gcgaaatctt tgtgcagccg gtctgggata cggagagcac gcagctgttt
      241 cgtacacgtt ttaaagccgt gagtccgaaa cgggtggaca cgccaggaca cggtatgggg
      301 aaccgttt
//
LOCUS       DAZGBC010000763          305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.767, whole genome shotgun sequence.
ACCESSION   DAZGBC010000763 DAZGBC010000000
VERSION     DAZGBC010000763.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.767"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>305)
                     /locus_tag="ACHK1J_005805"
     CDS             complement(<1..>305)
                     /locus_tag="ACHK1J_005805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7914888.1"
                     /translation="LGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLNAMYHKKQ
                     NAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKLQL"
BASE COUNT           62 a           94 c           74 g           75 t
ORIGIN      
        1 cagttgcagt ttgtccgccg cccctgatgc cggagatttc accagtgtga acggtgtacc
       61 ccgtttctca tccagtaccg gcgtcacctg aatgctgttg tttccggcaa actcaaaagc
      121 cagtgtgtgc catccgttat caaagacccc gaacgtatcc agcttcgcat tctgcttctt
      181 gtggtacatc gcgttcaggt tcgtcgcatc cgtctgcagg aagaaggaca tcagcatgtc
      241 gttgccttcc tctgacagcg tcactccctc cggcagggac gacaactgcc agtaaatacc
      301 caggg
//
LOCUS       DAZGBC010000764          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.768, whole genome shotgun sequence.
ACCESSION   DAZGBC010000764 DAZGBC010000000
VERSION     DAZGBC010000764.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.768"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..151)
                     /locus_tag="ACHK1J_005806"
     CDS             complement(2..151)
                     /locus_tag="ACHK1J_005806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7914889.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
BASE COUNT           79 a           84 c           66 g           74 t
ORIGIN      
        1 cttacttccg tgcttcgtag gccagagtta cctgcaccac tgtatttccc ctgtgaatat
       61 tcagctcaca taaccgttcc ctgaatatca gtgaaaaaca caacaccgtg gcgcacacgg
      121 cgagcaaccc gataagggca tatttcgtca taaactttga cctctctgca aggaagaggc
      181 tacaatcagt agtgttcaag tacttcatgc aggcctcacg agttaatgga ttaacaagtg
      241 gggtcttcgc atttctggta cctgcgtctt gcctgaacca gaaaggctca gacacccgcc
      301 gcg
//
LOCUS       DAZGBC010000765          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.769, whole genome shotgun sequence.
ACCESSION   DAZGBC010000765 DAZGBC010000000
VERSION     DAZGBC010000765.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.769"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>303)
                     /locus_tag="ACHK1J_005807"
     CDS             complement(<1..>303)
                     /locus_tag="ACHK1J_005807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO7914890.1"
                     /translation="QLNWMLELNKWKEERILTGEIHRPECRNEAAKRINCAFLSKQND
                     IDLSGLNLTTQPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQ"
BASE COUNT           82 a           48 c           63 g          110 t
ORIGIN      
        1 cttgaggaaa atttattgac tcaagagcgt tactattcag actaagtttt acgagtctat
       61 cgtagttggt tgcatcaaaa tgtgtgagtt ggttattatc aagattgata gaggtgaagt
      121 tttgcagccc tggtggttga gtagttaaat taagtcctga taaatcaatg tcattctgtt
      181 tcgacaaaaa agcacagttt atccttttag cggcttcgtt tcgacattcc ggacgatgga
      241 tttcacctgt aagtatacgt tcttctttcc atttatttaa ttcgagcatc cagttaagtt
      301 gat
//
LOCUS       DAZGBC010000766          299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.770, whole genome shotgun sequence.
ACCESSION   DAZGBC010000766 DAZGBC010000000
VERSION     DAZGBC010000766.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.770"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>299)
                     /locus_tag="ACHK1J_005808"
     CDS             complement(<1..>299)
                     /locus_tag="ACHK1J_005808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7914891.1"
                     /translation="GAILSCQSGMWAGFESYPVGSPIPWPSATPPQGYLVMNGQSFSC
                     SRYPQLARAYPGCKLPDLRGVFIRGWDNGRGLDGDRNRHLLSYQADQSGVYHERG"
BASE COUNT           76 a           88 c           80 g           55 t
ORIGIN      
        1 ccccacgctc atgatagaca ccggattgat cggcctgata actcaggaga tgtctgttcc
       61 gatctccatc cagcccacgt ccgttgtccc agccacggat aaaaacaccg cgcaaatccg
      121 gcagcttaca cccgggataa gcacgggcaa gctgaggata ccgggaacat gaaaatgact
      181 gcccgttcat caccaggtag ccttgtggag gggtggctga tggccacggt atagggcttc
      241 cgacagggta ggattcaaaa cctgcccaca taccggattg gcacgaaagt atggcccct
//
LOCUS       DAZGBC010000767          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.771, whole genome shotgun sequence.
ACCESSION   DAZGBC010000767 DAZGBC010000000
VERSION     DAZGBC010000767.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.771"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..180
                     /locus_tag="ACHK1J_005809"
     CDS             <1..180
                     /locus_tag="ACHK1J_005809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914892.1"
                     /translation="KYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIHSTNSDYY
                     SGFYHTDVLIPGELA"
BASE COUNT           92 a           54 c           48 g          104 t
ORIGIN      
        1 aagtattttc tgaatattat gatagcagat ttcatcattc ccgcaccaga aaaaatatca
       61 accacatggc actcagatac taaaatgaat cgctttgctt gccctataca ttctactaat
      121 agtgattact attcaggttt ttatcataca gatgtattaa ttcccggaga actggcttag
      181 gacgccatca taatccatga tgttgatagt aatgatccta tgatttttaa ctggaatgta
      241 ttgttttatg tctcactgga aaagtttcct gttggcaatc cttattatgg agaaattc
//
LOCUS       DAZGBC010000768          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.772, whole genome shotgun sequence.
ACCESSION   DAZGBC010000768 DAZGBC010000000
VERSION     DAZGBC010000768.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.772"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..>297
                     /locus_tag="ACHK1J_005810"
     CDS             14..>297
                     /locus_tag="ACHK1J_005810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7914893.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINVVCDNGY
                     SLRVVDKSNQQSTSTPPPFTALAGIRCSTAHITAKDNAWLYSLSHQTSDTG"
BASE COUNT           77 a           82 c           75 g           63 t
ORIGIN      
        1 atctggaggc gaaatgatga aactggccct gacactggaa gccgacagcg ttaacgtaca
       61 ggcactgaac atggggcgca ttgtcgttga cattgatggt gttaatctcg ctgaactgat
      121 taacgtggtc tgcgataacg gttattcact ccgcgtggtc gataaatcca accaacagtc
      181 aaccagtaca ccaccaccgt ttacggccct tgctggcata cgctgcagta ccgcgcatat
      241 cacggcaaag gacaacgcct ggctgtactc gctgtcacat cagaccagtg acaccgg
//
LOCUS       DAZGBC010000769          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.773, whole genome shotgun sequence.
ACCESSION   DAZGBC010000769 DAZGBC010000000
VERSION     DAZGBC010000769.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.773"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           78 a           52 c           53 g          114 t
ORIGIN      
        1 tgtgcttgtt gtttttcaat tagctcaatt ttattgttat gttcttcttg ttgtttcagt
       61 aaactacttt ctggcttgcc ttcttcaatg tagtcacatc ccgttaatgt gcagagaact
      121 gaaacagtga cagcactgaa aaaaatcttc ttcaagttca tgtaaaaatc cttattgcct
      181 ccgaccagcg atcaatgccg gtagagttgt ccattgtatt attggtgatc tatgttgctt
      241 tacattaatg acagtgatgg gtgtcgatta aatattgttt taagcattca tcataaa
//
LOCUS       DAZGBC010000770          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.774, whole genome shotgun sequence.
ACCESSION   DAZGBC010000770 DAZGBC010000000
VERSION     DAZGBC010000770.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.774"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>295)
                     /locus_tag="ACHK1J_005811"
     CDS             complement(<1..>295)
                     /locus_tag="ACHK1J_005811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO7914894.1"
                     /translation="PDFSVLINIYGERYFGGEIIDTDARTELGEGVLHIAVRKRRQLD
                     VRCFIQHGADINAKANNGFDFTPLHYSIGIGDFDMMKLLLKLGADIYLESEMGY"
BASE COUNT          100 a           75 c           34 g           86 t
ORIGIN      
        1 agtatcccat ctcactctcc aaataaatat cagcacctaa tttcaacaaa agcttcatca
       61 tatcaaaatc ccctatccct atcgaataat gcaaaggagt gaaatcaaag ccattatttg
      121 ccttagcatt tatatcggca ccatgttgaa taaaacatct gacatctaat tgtcttcttt
      181 tcctaacagc tatatgaaga accccctcgc caagttctgt ccttgcatct gtatctataa
      241 tctctccacc aaaatatcgc tcaccgtaga tattaataag tacagaaaaa tcggg
//
LOCUS       DAZGBC010000771          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.775, whole genome shotgun sequence.
ACCESSION   DAZGBC010000771 DAZGBC010000000
VERSION     DAZGBC010000771.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.775"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>294)
                     /locus_tag="ACHK1J_005812"
     CDS             complement(<1..>294)
                     /locus_tag="ACHK1J_005812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7914895.1"
                     /translation="FDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFDDPESLGF
                     AGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCYLWL"
BASE COUNT           75 a          110 c           66 g           43 t
ORIGIN      
        1 agccagagat aacagctgcc cccgtcatcc ggagaaacac gatccaccca gaacatctcg
       61 ccgttaatgg tcagcgtgtc accacgccgc acagcacgca ccgtatccgt ccgcacaaat
      121 aatgacgggc tgcttccttc aatacggacc ccgccaccgg caaaacccag cgactccgga
      181 tcgtcaaaaa ccccctgaac ttcgccgcca cgctgtgcac cggaggtgaa ctgcgcacgg
      241 atccccatca cttcaacgat cgtactgtcc accccggcga gggcagcatc aaag
//
LOCUS       DAZGBC010000772          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.776, whole genome shotgun sequence.
ACCESSION   DAZGBC010000772 DAZGBC010000000
VERSION     DAZGBC010000772.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.776"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..>294
                     /locus_tag="ACHK1J_005813"
     CDS             14..>294
                     /locus_tag="ACHK1J_005813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7914896.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVG"
BASE COUNT           80 a           76 c           74 g           64 t
ORIGIN      
        1 atccggagaa acgatgaaac tttccctgat gctggaagcc gacagaatta atgtgcaggc
       61 actgaacatg gggcaaattg tcgttgacgt cgatggtgtt aatctcactg aactgattaa
      121 caaggtcgct gaaaacggtt attcactccg cgtggtggag gaatccgacc aacagtcaac
      181 ctgcacacta ccaccgtttg caacccttgc cggcatacgc tgcagtaccg cacatatcac
      241 ggaaaaggat aacgcctggc tgtactcgct gtcgcatcag accagtgacg ttgg
//
LOCUS       DAZGBC010000773          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.778, whole genome shotgun sequence.
ACCESSION   DAZGBC010000773 DAZGBC010000000
VERSION     DAZGBC010000773.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.778"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           84 a           50 c           83 g           73 t
ORIGIN      
        1 actgggaggg gagagaaaac cggggcttcg ttctgttaga tcctggacgg gggagctttc
       61 gcatgatcga attaaatggt ctgaacttcc accagtagct tcacccgagg gggtgtcagg
      121 agcatatgcc tctgttattg atggaaatat ctttctggcc ggtggtgcat atttcccggg
      181 ggcggcagaa aagtatagca atggtgaata ctggtctcat aaaggactgg ataaagccta
      241 ttcaaaagag atatatcagc taataaaaaa tgactggaaa aaagttggtt
//
LOCUS       DAZGBC010000774          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.779, whole genome shotgun sequence.
ACCESSION   DAZGBC010000774 DAZGBC010000000
VERSION     DAZGBC010000774.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.779"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..>290
                     /locus_tag="ACHK1J_005814"
     CDS             5..>290
                     /locus_tag="ACHK1J_005814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914897.1"
                     /translation="MFGVIMKTKTFRFRKYKKKNVLLQMIVSRKQVILLRNIMTDSLL
                     FCSVGFVFMNMCDSKYTLISGEGGLRADCADYLFFAVYAIALLMIILWMTA"
BASE COUNT           82 a           37 c           63 g          108 t
ORIGIN      
        1 attcatgttc ggggtgatta tgaaaactaa aacattccgg ttccggaaat ataaaaagaa
       61 aaacgtcctg ctgcaaatga tagtttccag aaaacaagtg atattgttac gcaatattat
      121 gacggactct ttattatttt gctcggtagg ttttgtgttt atgaatatgt gtgattcaaa
      181 atatacgttg atttctggtg agggtggttt acgtgctgat tgtgccgatt atttattctt
      241 tgccgtatat gcaattgcgt tgttaatgat aattttatgg atgacagctt
//
LOCUS       DAZGBC010000775          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.780, whole genome shotgun sequence.
ACCESSION   DAZGBC010000775 DAZGBC010000000
VERSION     DAZGBC010000775.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.780"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>290
                     /locus_tag="ACHK1J_005815"
     CDS             <1..>290
                     /locus_tag="ACHK1J_005815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914898.1"
                     /translation="LGDTPLVDFTRDRLHRETLRSFGHYELTTAYTPAGQLQSQHLNS
                     LQYDRDYTWNDNGELIRISSPRHTRSYSYSNSGRLTGVHTTTSTLDIRIPYAT"
BASE COUNT           67 a          110 c           67 g           46 t
ORIGIN      
        1 ctcggcgaca caccgctggt ggatttcacc cgcgaccgcc tgcaccggga aacgctgcgc
       61 agcttcggcc attatgaact caccaccgct tatacccctg ccgggcagtt acagagccag
      121 cacctgaaca gcctgcagta tgaccgcgat tacacctgga acgacaacgg cgaactcatc
      181 cgcatcagca gcccgcgcca tacccggagt tacagctaca gcaactccgg caggctgacc
      241 ggcgttcaca ccaccacctc aactctggat atccgcatcc cgtatgccac
//
LOCUS       DAZGBC010000776          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.781, whole genome shotgun sequence.
ACCESSION   DAZGBC010000776 DAZGBC010000000
VERSION     DAZGBC010000776.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.781"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>288
                     /locus_tag="ACHK1J_005816"
     CDS             <1..>288
                     /locus_tag="ACHK1J_005816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914899.1"
                     /translation="KVAAAESAEKAAEDELVKVNQDGVVGKAEAERLTELNDAVTAAK
                     ADAQQAVDGLQDGPAKTGFSERLDKLDGITVPAVTDANDDGVADDKAAEVEA"
BASE COUNT           73 a           59 c          113 g           43 t
ORIGIN      
        1 aaaagtggct gcagcagagt ctgcagagaa agccgcggaa gatgaactgg tgaaagtgaa
       61 ccaggacggc gtggtgggta aagctgaagc cgagcgactg acggagctga atgatgcggt
      121 gacagcggcg aaagctgatg cccagcaggc ggtggatggt ctgcaggatg gtccggccaa
      181 gacgggcttc agtgagcgtc tggataaact ggacgggatc acggtaccgg cggtgacgga
      241 tgcgaacgac gatggtgtgg ctgatgacaa ggctgccgag gtcgaagc
//
LOCUS       DAZGBC010000777          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.782, whole genome shotgun sequence.
ACCESSION   DAZGBC010000777 DAZGBC010000000
VERSION     DAZGBC010000777.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.782"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHK1J_005817"
     CDS             complement(<1..275)
                     /locus_tag="ACHK1J_005817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7914900.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYK"
BASE COUNT           73 a           59 c           66 g           89 t
ORIGIN      
        1 tgcttataat ctgcaataaa attattaagc tgctcgtttc tgtccagcat gtcgttgatg
       61 tacggtaata gcgcggtgcc aaaaggtgtg agcatcagtt gtcgggtggt tcggtcaaaa
      121 acttttaaac ccacttttga ttcaaaatca gcaaggtatt tgctgacgtt ggcctgtgcg
      181 atgccaagta cagtcgcggc atggctgata ttttcactgg cagcgattac cgagataatt
      241 tttaactccc ggtacttaag ttgtaatttt gtcatcgcaa cagccca
//
LOCUS       DAZGBC010000778          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.783, whole genome shotgun sequence.
ACCESSION   DAZGBC010000778 DAZGBC010000000
VERSION     DAZGBC010000778.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.783"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>285
                     /gene="umuD"
                     /locus_tag="ACHK1J_005818"
     CDS             2..>285
                     /gene="umuD"
                     /locus_tag="ACHK1J_005818"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7914901.1"
                     /translation="MRPLFADRCQAGFPSPATDYAEQELDLNSYCISRPAATFFLRAS
                     GESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKRLLLRPRPAL"
BASE COUNT           59 a           81 c           83 g           62 t
ORIGIN      
        1 cgtgcgaccg ttgtttgccg atcgttgcca ggccggtttt ccttcgcctg ccactgatta
       61 tgctgagcag gaactggatc tgaacagcta ttgcatcagc agacctgcag ccaccttctt
      121 tctgcgcgcc agcggtgaat cgatgaacca ggctggcgtg cagaatggtg atctgctggt
      181 agtggacagg gccgaaaaac cacaacacgg ggacatcgtt atcgctgaga tcgacggtga
      241 gttcaccgtc aaacgactgc tgttgcgccc acgcccggca ctgga
//
LOCUS       DAZGBC010000779          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.784, whole genome shotgun sequence.
ACCESSION   DAZGBC010000779 DAZGBC010000000
VERSION     DAZGBC010000779.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.784"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>284
                     /locus_tag="ACHK1J_005819"
     CDS             <1..>284
                     /locus_tag="ACHK1J_005819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7914902.1"
                     /translation="MPQLTATEAAAQENQRVMGILTCQEAKGREQLATMLAGQQGMSV
                     EQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPDMTV"
BASE COUNT           71 a           72 c          102 g           39 t
ORIGIN      
        1 atgccgcaat taactgcaac ggaagccgcc gcgcaggaga accagcgagt gatggggatc
       61 ctgacgtgtc aggaagcgaa aggacgtgaa cagcttgcca cgatgctggc aggacaacag
      121 ggcatgagcg ttgaacaggc ccgggcgatt ctggccgcgg cagcaccgca gcagccggtg
      181 gcatccgcgc agagtgaagc cgatcgcatt atggcgtgtg aagaagctaa aggtcgtgaa
      241 caactggcgg caacgctggc ggcgatgccg gatatgacgg tgga
//
LOCUS       DAZGBC010000780          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.785, whole genome shotgun sequence.
ACCESSION   DAZGBC010000780 DAZGBC010000000
VERSION     DAZGBC010000780.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.785"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>283
                     /locus_tag="ACHK1J_005820"
     CDS             <1..>283
                     /locus_tag="ACHK1J_005820"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFO7914903.1"
                     /translation="ASSGVTDPALKKILRENAGLGTEATRAGILETLFARNYIERAGK
                     HIRSTQLGRELVAALPDSLTNPGMTALWEQALDDVARGKISLEAFMEKQ"
BASE COUNT           74 a           60 c           94 g           55 t
ORIGIN      
        1 atgcttcgtc tggtgtaacg gatccggcgc tgaaaaagat cctgagggag aatgcgggac
       61 tgggaacgga agccacacgc gcggggatcc tggaaacgtt atttgcgcgc aactatattg
      121 agagagcggg aaaacatatc cgttccacgc aactgggaag agaactggtg gcggcattgc
      181 cggactctct gacaaatccg ggtatgacgg ctttatggga acaggcgctg gatgatgttg
      241 cccgggggaa aatctcactg gaggcgttta tggagaaaca gca
//
LOCUS       DAZGBC010000781          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.786, whole genome shotgun sequence.
ACCESSION   DAZGBC010000781 DAZGBC010000000
VERSION     DAZGBC010000781.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.786"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>282
                     /locus_tag="ACHK1J_005821"
     CDS             2..>282
                     /locus_tag="ACHK1J_005821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014714112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914904.1"
                     /translation="MTGYACATEWISSEDLITSDFHLMTADERNVVKAATDDSMEAAY
                     MLKDNIRWYYHNGNLSLPANFSNQNKLVVNGNLTISGDYDDYLSGNGHL"
BASE COUNT           83 a           55 c           64 g           80 t
ORIGIN      
        1 tatgactggc tatgcatgtg ccacagagtg gatcagtagc gaggacctta tcacctctga
       61 ctttcatctt atgacggcag atgagcgcaa cgtggttaaa gcggcaaccg acgatagtat
      121 ggaagctgct tatatgctaa aagataatat ccgctggtat tatcataatg gtaacctatc
      181 attacccgct aacttcagca atcaaaataa actggtggtg aacggtaatt taacgatttc
      241 cggcgattat gatgattatc tgtcaggtaa tggtcatctg at
//
LOCUS       DAZGBC010000782          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.787, whole genome shotgun sequence.
ACCESSION   DAZGBC010000782 DAZGBC010000000
VERSION     DAZGBC010000782.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.787"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>281
                     /locus_tag="ACHK1J_005822"
     CDS             <1..>281
                     /locus_tag="ACHK1J_005822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914905.1"
                     /translation="LGDTPLVDFTRDRLHRETLRRFGRYELTTAYTPVGQLQRQHLNS
                     LQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIP"
BASE COUNT           64 a          104 c           70 g           43 t
ORIGIN      
        1 ctcggcgaca caccgctggt ggatttcacc cgcgaccgcc tgcaccggga aacgctgcgc
       61 agattcggcc gttatgaact caccaccgct tatacccctg tcgggcagtt acagcgtcag
      121 cacctgaaca gcctgcagta tgaccgcgat tacacctgga acgacaacgg cgaactcatc
      181 cgcatcagca gcccgcgcca gacccggagt tacagctaca gcaccaccgg caggctgacc
      241 ggcgttcaca ccaccgcagc gaatctggat atccgcatcc c
//
LOCUS       DAZGBC010000783          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.788, whole genome shotgun sequence.
ACCESSION   DAZGBC010000783 DAZGBC010000000
VERSION     DAZGBC010000783.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.788"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHK1J_005823"
     CDS             <1..207
                     /locus_tag="ACHK1J_005823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL/TraM family protein"
                     /protein_id="HFO7914906.1"
                     /translation="LVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRR
                     VDPRIKPGGMEISQMVSRNAASGT"
BASE COUNT           64 a           81 c           73 g           60 t
ORIGIN      
        1 ctggtgaatc agggccagat ggaaaacggc atctggttct ggaaattcca gtatccggtc
       61 aggatgcgtc tggtcggtca gaccagcacc cgccccgagc aggccttcac ctttgaaatc
      121 actgtccggc gcgttgaccc acgaatcaaa cccggcggca tggaaatcag ccagatggtt
      181 tcccgtaacg ccgcatcagg aacctgagac tcatatcatg caaatacatc atcttcttgg
      241 cgcactgctt attgtgtgcg gcactgtcac ggctgcag
//
LOCUS       DAZGBC010000784          277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.789, whole genome shotgun sequence.
ACCESSION   DAZGBC010000784 DAZGBC010000000
VERSION     DAZGBC010000784.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.789"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..182
                     /gene="yhhH"
                     /locus_tag="ACHK1J_005824"
     CDS             <1..182
                     /gene="yhhH"
                     /locus_tag="ACHK1J_005824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO7914907.1"
                     /translation="VKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSDQIAGGVFIIE
                     IGKNDGRILNFGHGK"
BASE COUNT          100 a           39 c           72 g           66 t
ORIGIN      
        1 atgttaagaa tcgttatgga caggatgccg cagaagaaga aaaaccatac gaaatcactg
       61 agttaacaac aagttgggtt gttgaaggta ccattcactc agaccaaatt gctggtgggg
      121 tttttattat agaaataggc aaaaatgatg ggagaattct gaattttggc cacggaaaat
      181 aagtatgtaa ggataatcaa acatcgtgtg cgctgatggc agagtaggtg gaggactcca
      241 gacagtcaaa cgatagaaaa agatagcctt tatggag
//
LOCUS       DAZGBC010000785          277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.790, whole genome shotgun sequence.
ACCESSION   DAZGBC010000785 DAZGBC010000000
VERSION     DAZGBC010000785.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.790"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>277
                     /locus_tag="ACHK1J_005825"
     CDS             <1..>277
                     /locus_tag="ACHK1J_005825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7914908.1"
                     /translation="EQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSR
                     RIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPR"
BASE COUNT           72 a           84 c           77 g           44 t
ORIGIN      
        1 ccgaacagga agcgctgatc cacgccctgc aggaaaagct gagcaaccgg gagcgcgaaa
       61 tagaccatct gcaggcgcag ctggataaac tccgccggat gaacttcggc agtcgttccg
      121 aaaaagtctc ccgccgtatc gcacaaatgg aagccgatct gaaccggctt cagaaagaga
      181 gcgatacgct gactggtagg gtgtatgacc cggcagtaca gcgtccgttg cgtcagaccc
      241 gcacccgtaa gccgttccct gaatcactac cccgtga
//
LOCUS       DAZGBC010000786          277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.791, whole genome shotgun sequence.
ACCESSION   DAZGBC010000786 DAZGBC010000000
VERSION     DAZGBC010000786.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.791"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(49..>277)
                     /gene="reiD"
                     /locus_tag="ACHK1J_005826"
     CDS             complement(49..>277)
                     /gene="reiD"
                     /locus_tag="ACHK1J_005826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFO7914909.1"
                     /translation="RRYGYSPMDYLIKRRLGEAQSLLTTDEGGCEKITSIAYRVGFSN
                     LSHFQNYFKNKVGKTPGQYRKDYRKANCLFF"
BASE COUNT           89 a           53 c           42 g           93 t
ORIGIN      
        1 ctacgcaggt gaattattta ctatgcgtta atttatgttg cattaatatt aaaaaaataa
       61 gcaattagct ttacgataat ctttacgata ttgtccaggc gtttttccta ctttattttt
      121 aaaatagttc tgaaaatgac tcaaattact gaaaccaaca cgataggcaa ttgaggtaat
      181 tttttcacac ccaccttcat cagtggtaag taaagattgt gcctccccta accgacgttt
      241 aatcaaatag tccattggcg aatatccata gcgccgt
//
LOCUS       DAZGBC010000787          275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.792, whole genome shotgun sequence.
ACCESSION   DAZGBC010000787 DAZGBC010000000
VERSION     DAZGBC010000787.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.792"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>275
                     /locus_tag="ACHK1J_005827"
     CDS             <1..>275
                     /locus_tag="ACHK1J_005827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7914910.1"
                     /translation="VLLSGLMQGTAAWRLRGVMFSPELTVAGAVPNTCPDTPAWKAII
                     DDCDAVSGRKLGFNWLKMLRLILLSLIVLWGAGTLLSLLVNRTQIYQ"
BASE COUNT           50 a           69 c           80 g           76 t
ORIGIN      
        1 tgttttgctc agtggactga tgcaggggac tgctgcctgg cgtttacgcg gtgtcatgtt
       61 cagtcctgaa ctgaccgttg ccggagcggt accaaacaca tgccccgaca cacctgcctg
      121 gaaagctatt attgatgact gtgatgccgt cagcggcagg aagctgggct ttaactggct
      181 gaagatgctg cgattgattc tgctttccct gattgtcctg tggggggccg gtacactgct
      241 gtctctgctc gttaaccgta ctcagattta tcagg
//
LOCUS       DAZGBC010000788          272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.denovo.793, whole genome shotgun sequence.
ACCESSION   DAZGBC010000788 DAZGBC010000000
VERSION     DAZGBC010000788.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.denovo.793"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>272)
                     /locus_tag="ACHK1J_005828"
     CDS             complement(<1..>272)
                     /locus_tag="ACHK1J_005828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO7914911.1"
                     /translation="SFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTR
                     HVMFFATTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIW"
BASE COUNT           66 a           71 c           75 g           60 t
ORIGIN      
        1 ccagattgtc ctcgtggtat cgttctgtgc cttgccgtca acaaaggtaa agcctcttcc
       61 ccagcagagc gggttgagca gccaggcaaa ggtggtttct gttttaccgc caccggtggt
      121 ggcaaaaaac atgacatgcc gggtgaggtc atccatgctc agccagagct cacgaccgat
      181 atcattgata cggcggtaac cgacataaaa aatcccccgt gcgggggatg tttcatctgc
      241 ttcatactga aacagggtgg gccagaagct ga
//
LOCUS       DAZGBC010000789        17614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.guided.099, whole genome shotgun sequence.
ACCESSION   DAZGBC010000789 DAZGBC010000000
VERSION     DAZGBC010000789.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.guided.099"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHK1J_005829"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHK1J_005829"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO7914912.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHK1J_005830"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHK1J_005830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO7914913.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHK1J_005831"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHK1J_005831"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO7914914.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHK1J_005832"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHK1J_005832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO7914915.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAGLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHK1J_005833"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHK1J_005833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO7914916.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHK1J_005834"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHK1J_005834"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO7914917.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="ACHK1J_005835"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="ACHK1J_005835"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO7914918.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="ACHK1J_005836"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="ACHK1J_005836"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO7914919.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="ACHK1J_005837"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="ACHK1J_005837"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO7914920.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="ACHK1J_005838"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="ACHK1J_005838"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO7914921.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12901..14034
                     /locus_tag="ACHK1J_005839"
     CDS             12901..14034
                     /locus_tag="ACHK1J_005839"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7914922.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="ACHK1J_005840"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="ACHK1J_005840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7914923.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHK1J_005841"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHK1J_005841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7914924.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15146..15292)
                     /gene="ecnB"
                     /locus_tag="ACHK1J_005842"
     CDS             complement(15146..15292)
                     /gene="ecnB"
                     /locus_tag="ACHK1J_005842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7914925.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15403..15528)
                     /gene="ecnA"
                     /locus_tag="ACHK1J_005843"
     CDS             complement(15403..15528)
                     /gene="ecnA"
                     /locus_tag="ACHK1J_005843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7914926.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15580..16146)
                     /gene="efp"
                     /locus_tag="ACHK1J_005844"
     CDS             complement(15580..16146)
                     /gene="efp"
                     /locus_tag="ACHK1J_005844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7914927.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16188..17216
                     /gene="epmB"
                     /locus_tag="ACHK1J_005845"
     CDS             16188..17216
                     /gene="epmB"
                     /locus_tag="ACHK1J_005845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7914928.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4381 a         4614 c         4544 g         4075 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 ggcgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccgacgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctggccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc actcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgaacagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga ctgtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcgc
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aacttggctc taccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacca gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccaga agtcatagag gcactccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagttattga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaacga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 ctgaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttgcgtaat caattgaaca
     3721 gccaacgcca gcttgaggca gagcgggcgc tggaaagtac cgaattacta gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggt gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacactac tgctggaact gaccaagctg aaagtctcta
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctattttacc cgttttctcg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatacc gctgtatctc aacaaagagg gcagcggcga caatattacc aaccatatgt
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcatgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcacgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat tagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtgga aagtctggag ccgattaccc
     5521 tcggtgcggg gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaatc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtaccggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttactggt agcgtgacaa
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggctggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc actgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatggcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgcca aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccccc caccgcagta atgcgggcaa ttttattaat cccctttgaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtgctcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagataccg
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac
     8041 gcgcccactg aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcacccagcg
     8281 ccaacatcac cagacgatca acgcctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttacc aatatttggt tctacgccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagc gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaaac tgcggcacag ctggaatacc ggcccgcaac
     8941 cggcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaataa aacaacggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgtac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggttgc cgtggcgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagtct ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggcgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 gctgcacggt gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaagatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gctatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggctg agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctacc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttattg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtagttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggttggta ttctgctgcc
    12601 gctggggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aatccacgta cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg
    12841 catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atcccgggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaacgggatc
    13261 acgctattac atctcgcaac ctacaccgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggcgcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga cagcatcatt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaagcgat tacgcctcca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctgca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctcc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtgaccg tagtgaataa
    14221 tttcgacgcc aaacgttatc tcggtacctg gtatgagatt gcccgttttg atcaccgctt
    14281 tgagcgtgga ctggaaaaag tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag aggaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggccc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaaattta tttgggtaca
    14641 gcagcccggc agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt
    14821 catcgcccag gcaagtagcg ccatactgac aatcatcgcc gtcacggtaa taacactcgg
    14881 cgtcaaacga ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga cgcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattacctt tgtgactatc gcagagaaaa caaacggcac
    15121 gacacagctg tcgtaccgtt attgcttatt gctgcgcttt cgttgctgca ccagaaatcg
    15181 cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata
    15241 ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt
    15301 ccttttaggt aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca
    15361 aaaatgatgg aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcggga
    15421 gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg cgagcggtgt tacagcccgt
    15481 gagcagcgtg ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa
    15541 taagtgatgg tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta
    15601 ttcaccagag cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt
    15661 aaccacagcg ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt
    15721 caggcccgga tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat
    15781 cggctgacca ttccacagag ttacgataca ctctgcctga tccagcagcc atttcgcgtt
    15841 gtcgccaatt gcttttgcgt cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg
    15901 ccagaactca ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc
    15961 agcggaatca gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag
    16021 tttaacgcga gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc
    16081 gtaaggttcg ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt
    16141 tgccataagg ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg
    16201 taaccctaaa taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg
    16261 atcctgatga acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac
    16321 gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga
    16381 aaggcaatcc ggacgatccg cttttgcgtc aggtaattac ctcgcaagat gagtttgcca
    16441 tcgcgcccgg attctccacc gacccgctgg aagaacagca cagcgtagtg cctggtttgt
    16501 tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc
    16561 gttattgctt ccgtcgccac ttcccttatg cagaaaatca gggcaacaag cgtaactggc
    16621 aaactgcact tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg
    16681 gcgatccgct gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca
    16741 tcccgcatat caaacgtctg cggattcaca gtcgtctgcc gattgtgatc ccggcacgca
    16801 tcacagatgc gctggttgaa cgcttttcac attctacgct gcaaatcttg ctggtgaatc
    16861 acatcaacca tgccaatgag atagatgaaa cattccgtca ggcgatggct aagttgcgcc
    16921 gggtaggcgt tactttgctg aaccagagcg ttctgttacg tgatgtgaac gataacgcac
    16981 aaacgctggc aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg tattacctgc
    17041 atgtgctcga taaagtacag ggtgcggcgc attttatggt gagtgatgac gaagcacggc
    17101 agattatgcg tgagttgctg acactggtgt cgggatatct ggtgccgaaa ctggcgcgag
    17161 aaattggcgg cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga
    17221 ttaaacaggg aagtcaaaca ttaaaattgc ctgatgcgct acgcttatca ggcctacatc
    17281 gtgcattgaa attgaatgga tttgcaatga actgtaggcc ggataaggcg tttacgccgc
    17341 atccggcaag agcaacaaac gcaagtacag caacctgtca ttaaacaggg aagtttaatc
    17401 tttgcccgtt gcataacctt taattattca tatgcattgc tgttgttact ctttatataa
    17461 ctaacaccaa taattaacct ttcttttctg tttatataga gtgagtacgg ctatttatgt
    17521 tattttaaca actattacta ccaagcccat ttatctggct tataaattaa aattaattat
    17581 ttaattgaga aatggttagg gagaacctac atgg
//
LOCUS       DAZGBC010000790         6058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.guided.234, whole genome shotgun sequence.
ACCESSION   DAZGBC010000790 DAZGBC010000000
VERSION     DAZGBC010000790.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.guided.234"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..245
                     /gene="yafN"
                     /locus_tag="ACHK1J_005846"
     CDS             <1..245
                     /gene="yafN"
                     /locus_tag="ACHK1J_005846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO7914929.1"
                     /translation="NPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKP
                     IKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            248..646
                     /gene="yafO"
                     /locus_tag="ACHK1J_005847"
     CDS             248..646
                     /gene="yafO"
                     /locus_tag="ACHK1J_005847"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO7914930.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            656..1108
                     /locus_tag="ACHK1J_005848"
     CDS             656..1108
                     /locus_tag="ACHK1J_005848"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7914931.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            1299..1507
                     /locus_tag="ACHK1J_005849"
                     /pseudo
     CDS             1299..1507
                     /locus_tag="ACHK1J_005849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            1527..1835
                     /locus_tag="ACHK1J_005850"
                     /pseudo
     CDS             1527..1835
                     /locus_tag="ACHK1J_005850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(1892..3349)
                     /gene="pepD"
                     /locus_tag="ACHK1J_005851"
     CDS             complement(1892..3349)
                     /gene="pepD"
                     /locus_tag="ACHK1J_005851"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7914932.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3610..4068
                     /gene="gpt"
                     /locus_tag="ACHK1J_005852"
     CDS             3610..4068
                     /gene="gpt"
                     /locus_tag="ACHK1J_005852"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7914933.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4160..5404
                     /gene="frsA"
                     /locus_tag="ACHK1J_005853"
     CDS             4160..5404
                     /gene="frsA"
                     /locus_tag="ACHK1J_005853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7914934.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            5462..5863
                     /gene="crl"
                     /locus_tag="ACHK1J_005854"
     CDS             5462..5863
                     /gene="crl"
                     /locus_tag="ACHK1J_005854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7914935.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5902..>6058)
                     /locus_tag="ACHK1J_005855"
     CDS             complement(5902..>6058)
                     /locus_tag="ACHK1J_005855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7914936.1"
                     /translation="DLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVA
                     VGMTYQF"
BASE COUNT         1533 a         1464 c         1513 g         1548 t
ORIGIN      
        1 aaaacccagc taaatacttt attgatcaac cggttgcggt tctttctaat aatcgccccg
       61 caggatatct cttaagtgcc agcgcattcg aagcgttaat ggacatgctt gctgaacaag
      121 aggagaaaaa gcccataaag gcgcgcttcc gtccaagtgc tgcaagatta gaggaaatta
      181 cacgccgcgc tgaacaatat cttaatgata tgacggatga tgatttcaat gactttaagg
      241 aataaggatg cgggtattca aaacaaaact tattcgcctg caacttacag cagaggaact
      301 tgatgcgtta acggcggatt ttatttccta taagcgtgac ggtgttttgc cagatatatt
      361 tggtcgcgat gcactctacg acgactcgtt tacctggcca ttaatcaaat ttgagcgagt
      421 tgctcatatt catctggcaa atgtgaataa tccatttccg ccacagttgc gccaattcag
      481 cagaacgaat gacgaagcgc atttggtata ttgtcagggt gcgtttgatg agcaagcatg
      541 gttgctcatt gccattctga aacctgaacc tcataaactg gctcgagata acaaccaaat
      601 gcataaaatt gggaaaatgg cagaagcgtt tcgcatgcgt ttttgaattt atattatgaa
      661 taacatacaa ataagaaact atcagcctgg cgattttcag caactatgcg ctattttcat
      721 tagagcggtt acgatgacag ccagtcagca ttattcacca caacaaattg ctgcctgggc
      781 gcagattgac gaatctcgct ggaaggagaa actcgcgaaa tcacaagtgc gggttgcagt
      841 cattaatgca caaccggttg gttttatttc ccgcattgaa cattatatcg atatgttatt
      901 tgttgaccct gaatacaccc gccgtggggt tgccagtgct ttgttaaaac cttggattaa
      961 gtctgaatcc gaacttacgg tggacgccag cataaccgca aaaccctttt ttgaacgtta
     1021 tggttttcag acagttaagc agcagcgcgt tgaatgccgg ggagcgtggt ttactaattt
     1081 ttatatgcga tataaaccgc aacattaaat ccagcttgca atgaaaataa cgcccgcctg
     1141 gtatgtgcgc gttttctgat tccacaaact gcaaggaggt aaatcatgac aaatccttta
     1201 tcaatgactc tttgcagacc tttccaggat taattctttt tttcttgccc tggattcgtc
     1261 tgccatttcc tgatttttat tcataaatgg aatggtacat gggcaaatat attcgtccct
     1321 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
     1381 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
     1441 tacacacccc ggacgcggct acccgattgg cgcagcgttc ttctctgttg gtcgttttta
     1501 cccggcaaga cgtcgcggta acggtgctgg aaaccgaagc cgggcgttat cctgacacgc
     1561 tgcgttcgtc ggggccgggc ggtcaaaatg tcaataaaac cgacttggcg gtacgcgcca
     1621 cgcatctggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
     1681 aacggctggc gcgattgctg attgcctgga ggctggagca gcagcaacag gaaaatagcg
     1741 cggcgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
     1801 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
     1861 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
     1921 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
     1981 gtggaccggt gattgttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
     2041 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacagac
     2101 gctgataggt ttcacgtacc agatgcatca ccggagagtt tgcgtccggc tgccagccag
     2161 gatatgcacc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
     2221 tcaccacgta gtctttaccg ctgtcgatca gagaacggat cagacagtga atttctacat
     2281 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
     2341 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
     2401 gagatttcgc aatcagggca gctttatcgt tcgcaacaga gtccagcaac aaggccagat
     2461 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
     2521 tcagggcgtc gactttatca gccgcgacag caatggtcgc aaaggcttca cgcgggatgg
     2581 cgttacgcag tgtgccgccg ttgaaatcaa taagacgcag atccagttct tctgcatgac
     2641 ccgccaggaa gcgcaccagc agtttgttgg cattaccgag gccaacgtgg atttcaccgc
     2701 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
     2761 cctcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgcg caacccatgt
     2821 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
     2881 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
     2941 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag cgcagaggcc ataccaatgc
     3001 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
     3061 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
     3121 ggtgggcctg caagacgacc ggtttacgat tttccatacc tgctgtagca ggtttacgaa
     3181 tcaagatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
     3241 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
     3301 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
     3361 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
     3421 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     3481 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     3541 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     3601 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     3661 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     3721 ggcggtctgg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     3781 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     3841 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     3901 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatctttgc aaaaccggct
     3961 ggtcgtccgc tggttgatga ttatgttgtt gatatcccgc aagatacctg gatcgaacag
     4021 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4081 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     4141 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     4201 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     4261 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     4321 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     4381 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     4441 cggcaactgg atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtgc
     4501 ggaagaagat ccgcaactca gtggtcgtca ctggctgcat gcggctacgt tgtacaacat
     4561 tgccgcctat cctcatctga aaggagatga cctggccgag caagcgcagg ctttgtcaaa
     4621 ccgcgcctat gaagaggccg ctcagcgtct gccgggcacg atgcggcaga tggagtttac
     4681 cgtacccggc ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt
     4741 cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta
     4801 tgaacgttat tttgcgccgc gcggcattgc gatgctgact attgatatgc cgtcggtggg
     4861 cttttcatca aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgtcttaaa
     4921 ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt
     4981 cggcgctaac gttgccgtgc gtctggcata tcttgaatcg ccgcgtctga aagcggttgc
     5041 ctgtcttggt ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc
     5101 ggaaatgtat cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgatgaagc
     5161 gttgcgcgtg gagctgaatc gctattcatt aaaagtgcaa ggattgctgg gacgtcgctg
     5221 cccaacgcca atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc
     5281 acgcttaatc acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt
     5341 gtatcggaat tttgacaaag gtcttcagga aatcaccgac tggatcgaaa aacgcttgtg
     5401 ttaaaaattt gctaaatttt gccaatttgg taaaacagtt gcatcacaac aggagatagc
     5461 aatgacgtta ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg
     5521 cccgtatatt cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt
     5581 atgcgtcaac gtgaaaccgg caccggaagt gcgtgagttc tggggctggt ggatggagct
     5641 tgaagcgcag gaatcccgtt ttacctacag ttaccagttt ggtctgttcg ataaagcagg
     5701 cgactggaag agcgttccgg taaaagacac tgaagtggtt gaacgactgg agcacaccct
     5761 gcgtgagttt cacgagaaac tgcgtgaact gctgacgacg ctgaatctga agctggaacc
     5821 ggcggatgat tttcgtgacg agccggtgaa gttaacggcg tgagtgaaat gtgccggatg
     5881 catcacatcc ggcaataatc attaaaactg ataggtcatg ccaaccgcga caatatcatc
     5941 attattaata ttcaatttgt tatcgctatc cagttggttg attttataat caacaaacgc
     6001 tgacatattt ttgttgaaat aatatgtagc cccgacatcg atataattga ccagatct
//
LOCUS       DAZGBC010000791         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.guided.440, whole genome shotgun sequence.
ACCESSION   DAZGBC010000791 DAZGBC010000000
VERSION     DAZGBC010000791.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.guided.440"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK1J_005856"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK1J_005856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO7914937.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK1J_005857"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK1J_005857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO7914938.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          364 a          252 c          303 g          358 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattat tgagcaaaat aatttatatg tggccggatt tgttaatacg gcaacaaata
      361 ctttctacag attttcagat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttctgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggaga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcgcgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZGBC010000792         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2305F11843
            SAMN43283720-rid23083603.guided.441, whole genome shotgun sequence.
ACCESSION   DAZGBC010000792 DAZGBC010000000
VERSION     DAZGBC010000792.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283720
            Sequence Read Archive: SRR30325111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351373.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351373.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:33:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,859
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,591
            CDSs (with protein)               :: 5,591
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 36 of 179
            Pseudo Genes (multiple problems)  :: 43 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283720-rid23083603.guided.441"
                     /serotype="O 27:H30"
                     /isolate="2305F11843"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-05"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK1J_005858"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHK1J_005858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO7914939.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK1J_005859"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHK1J_005859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO7914940.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          363 a          253 c          302 g          359 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattgt tgagcaaaat aatttatatg tagccggatt cgttaatacg gcaacaaata
      361 ctttctacag attttctgat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttttgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggcga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcacgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
